LOCUS       DACADF010000001       111274 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.001, whole genome shotgun sequence.
ACCESSION   DACADF010000001 DACADF010000000
VERSION     DACADF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 111274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 111274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..111274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.001"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(31..312)
                     /locus_tag="ISB30_000001"
     CDS             complement(31..312)
                     /locus_tag="ISB30_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAP1987691.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(575..1684)
                     /gene="apbC"
                     /locus_tag="ISB30_000002"
     CDS             complement(575..1684)
                     /gene="apbC"
                     /locus_tag="ISB30_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAP1987692.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1816..3849
                     /gene="metG"
                     /locus_tag="ISB30_000003"
     CDS             1816..3849
                     /gene="metG"
                     /locus_tag="ISB30_000003"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP1987693.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3990..7790
                     /locus_tag="ISB30_000004"
     CDS             3990..7790
                     /locus_tag="ISB30_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HAP1987694.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHVSWGKVGTNGQNQVKSFAD
                     AAAAEKAKLKLIAEKTKKGYVENASANVHITPITKATPEVETSPESKNQRPWLADDAV
                     IRLTDDINRFAFPHRSRPREINYLHKDSQIWQRIANNTRAYDPANNYRSYPENWQQAF
                     AELQRRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLIIRCGMESAVEIALLALQL
                     RYKPVKGAVTTIIPPNHEAESLPSWHQRLCHHLSLASEDEWQRCVDKVLAAITTLSPA
                     RQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDEAALTAAEKYDFPPLYRD
                     FRAYLAMLLANNGVSGVSRILTEFVEEHSDNPTYLFERISETEDLVKWLWKTNHPDAI
                     QILILGVIGKKKHLEYLSKACQKHPAAAIAAYATLLAIHEDKEWHKALVKLITITPEL
                     VCDVIPWVSAKAAGILSECRPQSVAEECEYATADMLPELFVSPPWMTKEKKKNSPVFD
                     LPVLPVPSVSDVTPEIAKKLTRTYLVTRFQQIAQQQATKQILFTDLPLMEKASWERHL
                     VPLTPEQQILWCLGFDKWRESGEKIYEKIPAPQSAVDALLRFDFPALNAEFAQYHNNA
                     YKSWNLIALCYLPGQQAISFLNQIVKEDNYSGEGYILAIFGSAAIPAFMTCLQREPQR
                     LWFFTFFLGVNELALPMAQRLQKKMSYEDARNWLTDYPRHAAAGLLPVALGKQGKDRD
                     CARQALRLLVNLNQRETIEEIAQGYNQPDVLAALATLFDSEPLEEYPAKIAPLPGFYQ
                     FTLWRRPRLKSNNQPLPDDAMRHLGIMLSFPRDITPYAGLAIIKETFTRESLAEFGWD
                     LYTAWTEAGAPAKENWAFTSLGILGNDDTARKLTPLIRTWPGESQHKRAVYGLDVLAS
                     IGSDIALMLLNGIAKKIKFVALQEHACDKINMVAENRGLTMAELEDRLAPDLGLDSSG
                     SLTLDFGPRQFTVGFDETLKPVVRDANGKVLKDLPKPNQSDDKTLATDAVNLFKQLKK
                     DVRAIASQQIDRLEQAMCQRRRWTAEQFRLFLVEHPLVRHLTRRLLWGVYTEENTLIA
                     CFRVAEDSTYSDAQDELFTLPAGNIGIPHVLEISPESAAAFGQIYTDYEQLPPFRQLD
                     RGYYHLADNERDSHELIRWQGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYGDL
                     ELETEPFSLIYGETGYGDQLPVESVKITSPDNRYGKQSSLTFSALDDITASELINDIE
                     SLFD"
     gene            7803..11564
                     /locus_tag="ISB30_000005"
     CDS             7803..11564
                     /locus_tag="ISB30_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HAP1987695.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQENELHISWGKVGTNGQSQIKSFAD
                     AAAAEKAELKLIAEKTKKGYAEEVSVTTPTSAPVQVIACSEKAPLPQDKPPFAEDHLP
                     WLADDAQIILPTEVAPTTLSHRRWPGDSVPQENELTLLRSVAAHTHRRFKKVITFDYS
                     TCSLDWQQAITQAVGLIDSPISTALPPMVLAVLVALEQGFNRNDHEELMDQIVQEGGL
                     EYATEVVIALQAIRFDWNYDAHLITFTPDNKQPGYLSRFASVEMRLRKHLSLANDDVW
                     QRCADKLIAALGNIPAWHQPLVALLLPEKQDVSHEIARRLCGQKGLYALEWLKLTAAD
                     EQVLAELGKYYPGQPGQVFDDYYGGKIWCATILKEQGVGALARFAPYAAGDTCGEVLM
                     HINHPQALTLLIHASEQGKRCHDRMTKTFVRFPHAALAALAELLAQKDQKRWRMMLMT
                     MLISQPTLAERVIPWLSTPAVAVLKSCQQQLTQPSNHASADMLPAVLVSPPWLSKKKK
                     SVMPVLDLTPLPLESCCTLTETAEKEIHARHRWHAHQIDIGQKEDIQNYLTRLGFNRW
                     NNGQYMKASDAVVELWQRGDYSALISEFKTFWHSYQREWQLYMLAALPIEKTAQAWNV
                     LSKEPHVGVEFVMTHLQLAGLQGFIHSFSRYPQEALPVAQYFAAIELAPLIARAFNKL
                     KTLRQDARIWLLKYPEHAITGLLPAALGKTGEAQDNARAALRMLTENGHQPLLQEIAR
                     RYNQPEVTDAVNALLALDPLDNHPTKIPTLPTFYQPSLWTRPLLKANAQSLPDSALLH
                     LGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKESWAFTALGV
                     LGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQ
                     ERAKEKIANIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDETLKPFV
                     RDASGSRLKDLPKPNKSDDESQANNAVNRYKLLKKDARTVAAQQVARLESAMCLRRRW
                     SPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTADDDLFTLPEG
                     DISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASELTRWAG
                     RKCPSGRVMGLANKGWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSA
                     EQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE"
     gene            11574..15206
                     /locus_tag="ISB30_000006"
     CDS             11574..15206
                     /locus_tag="ISB30_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAP1987696.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDKKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPCLCTQAQAVVNGYLSASPKLAFESTHSNDLPEILVSPPWRGKKKTALLRLDLVPL
                     ELAPKARWQPGERERLAATESARYFSTGSFTERMERKSGRVVLQELGFGDDVWLFRNY
                     ILPGKLDAARKSLVGQWHYSPRRVEEINNGWHSTEAKSAEQALRSGDVEALINTWEND
                     SYSHYRQEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDTLPGLL
                     LAFSHHPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEQLLKLGPIEIYPA
                     RIPKAPDFWHPGMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYGGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDETTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRSLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRITRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            15267..15583
                     /locus_tag="ISB30_000007"
                     /pseudo
     CDS             15267..15583
                     /locus_tag="ISB30_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16285..17373
                     /locus_tag="ISB30_000008"
     CDS             16285..17373
                     /locus_tag="ISB30_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP1987697.1"
                     /translation="MSPQNNHLQRPPAAVLYAVELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNIRRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYLGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            17384..19663
                     /locus_tag="ISB30_000009"
     CDS             17384..19663
                     /locus_tag="ISB30_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987698.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSTLAHYFA
                     QLRGDSPGDALNRQREAFMARWITWAMQQNNGDVLVVCGGWHAPALAKMWRKCPQEMN
                     KPELSSLADGVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQCGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTVIDTLAGDGFGKLSPSTPQPPLPKDVTCELERTGISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGVVRQQGSTLTLAGNGEECWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGCITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLTQLPVSALQMLLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            19656..20792
                     /locus_tag="ISB30_000010"
     CDS             19656..20792
                     /locus_tag="ISB30_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP1987699.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERYGGLGRSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFISLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRYSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSSATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            20789..22792
                     /locus_tag="ISB30_000011"
     CDS             20789..22792
                     /locus_tag="ISB30_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987700.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDPAIWLKELANLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCVQEMARRDSIGEVPPMPWRTVVGAGIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVAL
                     REYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGEAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            22917..23378
                     /locus_tag="ISB30_000012"
     CDS             22917..23378
                     /locus_tag="ISB30_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAP1987701.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23419..23889)
                     /locus_tag="ISB30_000013"
     CDS             complement(23419..23889)
                     /locus_tag="ISB30_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP1987702.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(23936..24655)
                     /gene="yehT"
                     /locus_tag="ISB30_000014"
     CDS             complement(23936..24655)
                     /gene="yehT"
                     /locus_tag="ISB30_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAP1987703.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(24652..26337)
                     /gene="btsS"
                     /locus_tag="ISB30_000015"
     CDS             complement(24652..26337)
                     /gene="btsS"
                     /locus_tag="ISB30_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAP1987704.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            26559..27290
                     /gene="mlrA"
                     /locus_tag="ISB30_000016"
     CDS             26559..27290
                     /gene="mlrA"
                     /locus_tag="ISB30_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HAP1987705.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQTLLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            27350..27457
                     /locus_tag="ISB30_000017"
     CDS             27350..27457
                     /locus_tag="ISB30_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAP1987706.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(27438..28169)
                     /gene="yehW"
                     /locus_tag="ISB30_000018"
     CDS             complement(27438..28169)
                     /gene="yehW"
                     /locus_tag="ISB30_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAP1987707.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLKPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(28174..29100)
                     /gene="yehX"
                     /locus_tag="ISB30_000019"
     CDS             complement(28174..29100)
                     /gene="yehX"
                     /locus_tag="ISB30_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAP1987708.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLAMLTHPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALEERMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(29093..30250)
                     /gene="yehY"
                     /locus_tag="ISB30_000020"
     CDS             complement(29093..30250)
                     /gene="yehY"
                     /locus_tag="ISB30_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAP1987709.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGVQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(30257..31174)
                     /gene="osmF"
                     /locus_tag="ISB30_000021"
     CDS             complement(30257..31174)
                     /gene="osmF"
                     /locus_tag="ISB30_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAP1987710.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNVQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(31364..33661)
                     /gene="bglX"
                     /locus_tag="ISB30_000022"
     CDS             complement(31364..33661)
                     /gene="bglX"
                     /locus_tag="ISB30_000022"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAP1987711.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKAEFELL"
     gene            33857..35572
                     /gene="dld"
                     /locus_tag="ISB30_000023"
     CDS             33857..35572
                     /gene="dld"
                     /locus_tag="ISB30_000023"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAP1987712.1"
                     /translation="MSSMTTTDNKAFLNELSRLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(35611..36543)
                     /gene="pbpG"
                     /locus_tag="ISB30_000024"
     CDS             complement(35611..36543)
                     /gene="pbpG"
                     /locus_tag="ISB30_000024"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAP1987713.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(36717..37304)
                     /locus_tag="ISB30_000025"
     CDS             complement(36717..37304)
                     /locus_tag="ISB30_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAP1987714.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            37474..38052
                     /locus_tag="ISB30_000026"
     CDS             37474..38052
                     /locus_tag="ISB30_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP1987715.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(38182..38943)
                     /locus_tag="ISB30_000027"
     CDS             complement(38182..38943)
                     /locus_tag="ISB30_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1987716.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGALALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDGDVKPGAEPSIPLRRFGATHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(38996..40522)
                     /gene="mdtQ"
                     /locus_tag="ISB30_000028"
     CDS             complement(38996..40522)
                     /gene="mdtQ"
                     /locus_tag="ISB30_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAP1987717.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHLFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVEGVETDINASKTRQQ
                     LNDVAGKMKIIEARLSALTNHQTKALKLNPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGGGYKR
                     "
     gene            40656..40739
                     /gene="yohP"
                     /locus_tag="ISB30_000029"
     CDS             40656..40739
                     /gene="yohP"
                     /locus_tag="ISB30_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAP1987718.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(41127..42077)
                     /gene="dusC"
                     /locus_tag="ISB30_000030"
     CDS             complement(41127..42077)
                     /gene="dusC"
                     /locus_tag="ISB30_000030"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAP1987719.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            42316..42714
                     /locus_tag="ISB30_000031"
     CDS             42316..42714
                     /locus_tag="ISB30_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP1987720.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            42711..43406
                     /locus_tag="ISB30_000032"
     CDS             42711..43406
                     /locus_tag="ISB30_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAP1987721.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            43536..44420
                     /gene="cdd"
                     /locus_tag="ISB30_000033"
     CDS             43536..44420
                     /gene="cdd"
                     /locus_tag="ISB30_000033"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP1987722.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            44570..45289
                     /gene="sanA"
                     /locus_tag="ISB30_000034"
     CDS             44570..45289
                     /gene="sanA"
                     /locus_tag="ISB30_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAP1987723.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            45292..45531
                     /locus_tag="ISB30_000035"
     CDS             45292..45531
                     /locus_tag="ISB30_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAP1987724.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            45725..46963
                     /locus_tag="ISB30_000036"
     CDS             45725..46963
                     /locus_tag="ISB30_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP1987725.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIQQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     IAADKIILAVGQHAELAAFSALEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            46957..48192
                     /gene="preA"
                     /locus_tag="ISB30_000037"
     CDS             46957..48192
                     /gene="preA"
                     /locus_tag="ISB30_000037"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAP1987726.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGS
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(48435..49445)
                     /gene="mglC"
                     /locus_tag="ISB30_000038"
     CDS             complement(48435..49445)
                     /gene="mglC"
                     /locus_tag="ISB30_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAP1987727.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFIALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(49461..50981)
                     /gene="mglA"
                     /locus_tag="ISB30_000039"
     CDS             complement(49461..50981)
                     /gene="mglA"
                     /locus_tag="ISB30_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAP1987728.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            complement(51042..52040)
                     /gene="mglB"
                     /locus_tag="ISB30_000040"
     CDS             complement(51042..52040)
                     /gene="mglB"
                     /locus_tag="ISB30_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAP1987729.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(52320..53360)
                     /gene="galS"
                     /locus_tag="ISB30_000041"
     CDS             complement(52320..53360)
                     /gene="galS"
                     /locus_tag="ISB30_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAP1987730.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(53502..54659)
                     /locus_tag="ISB30_000042"
     CDS             complement(53502..54659)
                     /locus_tag="ISB30_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAP1987731.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAINNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLSLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            complement(54676..55344)
                     /gene="folE"
                     /locus_tag="ISB30_000043"
     CDS             complement(54676..55344)
                     /gene="folE"
                     /locus_tag="ISB30_000043"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAP1987732.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            55602..56438
                     /gene="yeiG"
                     /locus_tag="ISB30_000044"
     CDS             55602..56438
                     /gene="yeiG"
                     /locus_tag="ISB30_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HAP1987733.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYQMYDYLRDELPALIQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(56470..58461)
                     /gene="cirA"
                     /locus_tag="ISB30_000045"
     CDS             complement(56470..58461)
                     /gene="cirA"
                     /locus_tag="ISB30_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAP1987734.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTVQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(58753..60222)
                     /gene="lysP"
                     /locus_tag="ISB30_000046"
     CDS             complement(58753..60222)
                     /gene="lysP"
                     /locus_tag="ISB30_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAP1987735.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(60427..61308)
                     /locus_tag="ISB30_000047"
     CDS             complement(60427..61308)
                     /locus_tag="ISB30_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1987736.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            61407..62456
                     /locus_tag="ISB30_000048"
     CDS             61407..62456
                     /locus_tag="ISB30_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAP1987737.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGTNSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            62530..63387
                     /gene="nfo"
                     /locus_tag="ISB30_000049"
     CDS             62530..63387
                     /gene="nfo"
                     /locus_tag="ISB30_000049"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP1987738.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            63391..64479
                     /locus_tag="ISB30_000050"
     CDS             63391..64479
                     /locus_tag="ISB30_000050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1987739.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAIA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(64747..65688)
                     /gene="rihB"
                     /locus_tag="ISB30_000051"
     CDS             complement(64747..65688)
                     /gene="rihB"
                     /locus_tag="ISB30_000051"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAP1987740.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            65818..66516
                     /gene="yeiL"
                     /locus_tag="ISB30_000052"
     CDS             65818..66516
                     /gene="yeiL"
                     /locus_tag="ISB30_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAP1987741.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(66583..67833)
                     /locus_tag="ISB30_000053"
     CDS             complement(66583..67833)
                     /locus_tag="ISB30_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP1987742.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(67927..68865)
                     /locus_tag="ISB30_000054"
     CDS             complement(67927..68865)
                     /locus_tag="ISB30_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAP1987743.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(68853..69794)
                     /locus_tag="ISB30_000055"
     CDS             complement(68853..69794)
                     /locus_tag="ISB30_000055"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAP1987744.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            complement(70250..71941)
                     /gene="fruA"
                     /locus_tag="ISB30_000056"
     CDS             complement(70250..71941)
                     /gene="fruA"
                     /locus_tag="ISB30_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAP1987745.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDFIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(71958..72896)
                     /gene="fruK"
                     /locus_tag="ISB30_000057"
     CDS             complement(71958..72896)
                     /gene="fruK"
                     /locus_tag="ISB30_000057"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP1987746.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(72896..74026)
                     /locus_tag="ISB30_000058"
     CDS             complement(72896..74026)
                     /locus_tag="ISB30_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAP1987747.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            74394..75575
                     /gene="setB"
                     /locus_tag="ISB30_000059"
     CDS             74394..75575
                     /gene="setB"
                     /locus_tag="ISB30_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAP1987748.1"
                     /translation="MHNSPTVTSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(75572..75826)
                     /locus_tag="ISB30_000060"
     CDS             complement(75572..75826)
                     /locus_tag="ISB30_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAP1987749.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMIWLIDLEMLTAP"
     gene            75981..76553
                     /gene="yeiP"
                     /locus_tag="ISB30_000061"
     CDS             75981..76553
                     /gene="yeiP"
                     /locus_tag="ISB30_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAP1987750.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            76776..78242
                     /locus_tag="ISB30_000062"
     CDS             76776..78242
                     /locus_tag="ISB30_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP1987751.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGHTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATNESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     IFGDDLPDNPHFVQVIEQAWQQIAQFGAHQALLNTLKI"
     gene            78360..79346
                     /locus_tag="ISB30_000063"
     CDS             78360..79346
                     /locus_tag="ISB30_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP1987752.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            79385..80098
                     /gene="lpxT"
                     /locus_tag="ISB30_000064"
     CDS             79385..80098
                     /gene="lpxT"
                     /locus_tag="ISB30_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAP1987753.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPINHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            80510..81076
                     /gene="mepS"
                     /locus_tag="ISB30_000065"
     CDS             80510..81076
                     /gene="mepS"
                     /locus_tag="ISB30_000065"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAP1987754.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            81257..82813
                     /locus_tag="ISB30_000066"
     CDS             81257..82813
                     /locus_tag="ISB30_000066"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAP1987755.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            82895..84709
                     /locus_tag="ISB30_000067"
     CDS             82895..84709
                     /locus_tag="ISB30_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP1987756.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKISDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNTWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            84710..85804
                     /locus_tag="ISB30_000068"
     CDS             84710..85804
                     /locus_tag="ISB30_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAP1987757.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            85804..86829
                     /locus_tag="ISB30_000069"
     CDS             85804..86829
                     /locus_tag="ISB30_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1987758.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLLLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            86831..88420
                     /gene="yejF"
                     /locus_tag="ISB30_000070"
     CDS             86831..88420
                     /gene="yejF"
                     /locus_tag="ISB30_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAP1987759.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLHIEAGETLALVGES
                     GSGKSVTALSILRLLPCPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEKNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSHGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(88424..88768)
                     /locus_tag="ISB30_000071"
     CDS             complement(88424..88768)
                     /locus_tag="ISB30_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987760.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(89102..90292)
                     /gene="bcr"
                     /locus_tag="ISB30_000072"
     CDS             complement(89102..90292)
                     /gene="bcr"
                     /locus_tag="ISB30_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAP1987761.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(90320..91015)
                     /gene="rsuA"
                     /locus_tag="ISB30_000073"
     CDS             complement(90320..91015)
                     /gene="rsuA"
                     /locus_tag="ISB30_000073"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAP1987762.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            91165..92925
                     /locus_tag="ISB30_000074"
     CDS             91165..92925
                     /locus_tag="ISB30_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP1987763.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGSERDVLIDDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTILVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADVLAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            93050..93334
                     /gene="rplY"
                     /locus_tag="ISB30_000075"
     CDS             93050..93334
                     /gene="rplY"
                     /locus_tag="ISB30_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAP1987764.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(93473..94480)
                     /gene="yejK"
                     /locus_tag="ISB30_000076"
     CDS             complement(93473..94480)
                     /gene="yejK"
                     /locus_tag="ISB30_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAP1987765.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            94662..94889
                     /locus_tag="ISB30_000077"
     CDS             94662..94889
                     /locus_tag="ISB30_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAP1987766.1"
                     /translation="MPQISRYSDEQVEQLLAELLNILEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            94909..96669
                     /gene="pbgA"
                     /locus_tag="ISB30_000078"
     CDS             94909..96669
                     /gene="pbgA"
                     /locus_tag="ISB30_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAP1987767.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            96744..96820
                     /locus_tag="ISB30_000079"
     tRNA            96744..96820
                     /locus_tag="ISB30_000079"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96778..96780,aa:Pro,seq:ggg)
     gene            complement(96931..97874)
                     /locus_tag="ISB30_000080"
                     /pseudo
     CDS             complement(96931..97874)
                     /locus_tag="ISB30_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247409.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            98194..98841
                     /gene="narP"
                     /locus_tag="ISB30_000081"
     CDS             98194..98841
                     /gene="narP"
                     /locus_tag="ISB30_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAP1987768.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(98876..99928)
                     /locus_tag="ISB30_000082"
     CDS             complement(98876..99928)
                     /locus_tag="ISB30_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAP1987769.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(99925..100482)
                     /gene="dsbE"
                     /locus_tag="ISB30_000083"
     CDS             complement(99925..100482)
                     /gene="dsbE"
                     /locus_tag="ISB30_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAP1987770.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(100479..102422)
                     /locus_tag="ISB30_000084"
     CDS             complement(100479..102422)
                     /locus_tag="ISB30_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAP1987771.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAA"
     gene            complement(102419..102898)
                     /gene="ccmE"
                     /locus_tag="ISB30_000085"
     CDS             complement(102419..102898)
                     /gene="ccmE"
                     /locus_tag="ISB30_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAP1987772.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(102895..103104)
                     /gene="ccmD"
                     /locus_tag="ISB30_000086"
     CDS             complement(102895..103104)
                     /gene="ccmD"
                     /locus_tag="ISB30_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAP1987773.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(103101..103838)
                     /gene="ccmC"
                     /locus_tag="ISB30_000087"
     CDS             complement(103101..103838)
                     /gene="ccmC"
                     /locus_tag="ISB30_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAP1987774.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSILGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(103880..104542)
                     /gene="ccmB"
                     /locus_tag="ISB30_000088"
     CDS             complement(103880..104542)
                     /gene="ccmB"
                     /locus_tag="ISB30_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAP1987775.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(104539..105156)
                     /gene="ccmA"
                     /locus_tag="ISB30_000089"
     CDS             complement(104539..105156)
                     /gene="ccmA"
                     /locus_tag="ISB30_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAP1987776.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(105175..105777)
                     /gene="napC"
                     /locus_tag="ISB30_000090"
     CDS             complement(105175..105777)
                     /gene="napC"
                     /locus_tag="ISB30_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAP1987777.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(105787..106236)
                     /gene="napB"
                     /locus_tag="ISB30_000091"
     CDS             complement(105787..106236)
                     /gene="napB"
                     /locus_tag="ISB30_000091"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAP1987778.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(106233..107096)
                     /gene="napH"
                     /locus_tag="ISB30_000092"
     CDS             complement(106233..107096)
                     /gene="napH"
                     /locus_tag="ISB30_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAP1987779.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVIILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(107083..107778)
                     /gene="napG"
                     /locus_tag="ISB30_000093"
     CDS             complement(107083..107778)
                     /gene="napG"
                     /locus_tag="ISB30_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAP1987780.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(107785..110271)
                     /gene="napA"
                     /locus_tag="ISB30_000094"
     CDS             complement(107785..110271)
                     /gene="napA"
                     /locus_tag="ISB30_000094"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAP1987781.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(110268..110531)
                     /gene="napD"
                     /locus_tag="ISB30_000095"
     CDS             complement(110268..110531)
                     /gene="napD"
                     /locus_tag="ISB30_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAP1987782.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(110521..111015)
                     /gene="napF"
                     /locus_tag="ISB30_000096"
     CDS             complement(110521..111015)
                     /gene="napF"
                     /locus_tag="ISB30_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAP1987783.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGSYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
BASE COUNT        27440 a        29575 c        27619 g        26640 t
ORIGIN      
        1 tctaaatccg attaaaacca gggtggcaaa ttaatcgtag cgatttaaca cgattcgacg
       61 tttgctatcg ctatctgcag caatcacttc cgtcgttaca cctttaactg gcgaagaaac
      121 ctgatgacgg gtatcaacat tatacgttaa ctgaccacaa tgctgttgag gttgaccatt
      181 aaccatagcc atactgcatg tcgggggact gatcctacca ttaatcgtta atgtggcagt
      241 ttctgaagag aacgcgggag aagccaatcc ataggcaaga aaaataattc ctgacagcca
      301 gtacttattc attgaacgtt atccctgtag taaaggttat gcctggaaag aatgagtgca
      361 taacaaacta tagctgtaca tccactacac agccacgaag gatgataatg aagcatcgcc
      421 ggtacgatca atcgactttg tagagtttcg gacgaaggtc cgcagaatat tcgcaacatt
      481 aaataagtgt tcattaatgt tgcaaaaaca aatacaccgc atcaatgtaa catctctgcg
      541 gtgtatgaag aataaccaga atggttaata cgcgttagac cgcgcggaag gaaatctcgc
      601 caggaatgac ttcaccctgc cagtagagct gagctgcaac gcggtcagcc agctggcggt
      661 agatagcggt aaattcgctc tcaggacggc tgataaccgt tggcgtgcct ttatcgagat
      721 cctcacgtaa ggagatgtgt aacggcatct gccccagcag ctgcgtgtgg tatttctcgg
      781 ccagtttctc tgcaccaccg gtaccgaaaa tcggctcatg gtgaccacag ttactgcaaa
      841 tatgcacact catgttctcg acaataccga gcaccggaac ctcgactttc tcgaacatca
      901 caatgccttt tttcgcatcg atcaacgcga tgtcctgcgg ggtggtgacc actaccgcgc
      961 cagttactgg aatgttttgt gccagtgtca attgaatgtc accggtacct ggcggcatat
     1021 caagcaccag atagtcgaga tccggccaca aggtttcctg caacatttgc attaacgcct
     1081 tgctcgccat tgggccacgc cacaccatcg cattgtcgtc agtgaccaga taaccaattg
     1141 aattggttgc caggccatga gacataatcg gtgccatatg ggtaccgtcc ggcgaagttg
     1201 gacgctgatt ttctgcgccc agcatggtag ggattgatgg gccgtagata tcggcgtcca
     1261 gaatgccgac tttcgcccct tctgccgcca gtgccaacgc caggtttacc gccgtggagg
     1321 atttacccac gccaccttta ccggaactga ccgcgataat gttcttcacg ccgttaattc
     1381 ccggctgatt cttcacgcgt ttcagagtgg ctatgttatg cgacagtttc cagtcgatag
     1441 ctttcgctcc ggtgatgcgc agcagttcgg cgctacattg ctcttttaac tcttcgaacg
     1501 cgctatgcca gacgaatggc atgaccagtt ccacatgcag cgtgtcgtcc atccaggcga
     1561 catggtgcaa cgctttcagc gttgtgaggt tatgcttcag ggttgggtgc tgaaaattgg
     1621 ccagtgtccc ggcgaccatt gcgcgcaagg cttctggtga tttggcctgg gattgttcgt
     1681 tcatcccgac tccttttttg tatagataaa ccatcagctg atagtttacc tgaagaatat
     1741 agagaagtac ttacttaaca ttttcccatt tggtactatc taagcccttt tcactattaa
     1801 gaagtaatgc ctactatgac tcaagtcgcg aagaaaattc tggtgacgtg cgcactgccg
     1861 tacgctaacg gctcaatcca cctcggccat atgctggagc acatccaggc tgatgtctgg
     1921 gtccgttacc agcgaatgcg cggccacgag gtcaacttca tctgcgccga cgatgcccac
     1981 ggcacgccga tcatgcttaa agctcagcag cttggtatca ccccggagca gatgattggc
     2041 gaaatgagtc aggaacatca gactgatttc gcaggcttta acatcagcta tgacaactat
     2101 cactcgacgc acagcgaaga gaatcgccag ttgtcagaac ttatctactc tcgtctgaaa
     2161 gaaaacggtt ttattaaaaa ccgcaccatc tctcagctgt acgatccgga aaaaggcatg
     2221 ttcctgccgg accgttttgt aaaaggcacc tgcccgaaat gtaaatcccc ggatcaatac
     2281 ggcgataact gcgaagtctg cggcgcgacc tacagcccga ctgaactgat cgagccgaaa
     2341 tcggtggttt ctggtgctac cccggtaatg cgtgattctg aacacttctt ctttgatctg
     2401 ccctctttca gcgaaatgct gcaggcatgg acccgcagcg gtgcgttgca ggagcaggtg
     2461 gcaaataaaa tgcaggagtg gtttgaatct ggcctgcaac agtgggatat ctcccgcgac
     2521 gcgccttatt tcggttttga aattccgaac gcgccgggca aatatttcta tgtctggctg
     2581 gacgcaccga ttggctatat gggctctttc aagaatctgt gtgacaagcg cggcgacagc
     2641 gtaagcttcg atgaatactg gaagaaagat tccactgccg agctgtatca cttcatcggt
     2701 aaagatatcg tttacttcca cagcctgttc tggcctgcca tgctggaagg cagcaacttc
     2761 cgcaagccga ccaacctgtt tgttcacggc tatgtgacgg tgaacggcgc gaaaatgtcc
     2821 aagtctcgcg gcacctttat taaagccagc acctggctga atcattttga cgccgacagc
     2881 ctgcgctact actacaccgc caaactgtca tcgcgcattg atgatatcga tctcaacctg
     2941 gaagatttcg ttcagcgcgt gaatgccgat atcgtgaaca aagtggttaa cctggcgtcc
     3001 cgtaacgctg gctttatcaa taagcgtttt gacggtgtgc tggcaagcga gctggctgac
     3061 ccgcagttgt acaaaacctt cactgatgcc gctgaagtga ttggcgaagc gtgggaaagc
     3121 cgtgaatttg gtaaagctat ccgtgaaatc atggcgctgg ctgacctggc taaccgctat
     3181 gtcgatgaac aggctccgtg ggtggtggcg aagcaggaag gtcgcgatgc cgatctgcag
     3241 gcgatttgct caatgggcat caacctgttc cgcgtgctga tgacttacct gaaaccggta
     3301 ctgccgaaac tgaccgagcg cgcagaagca ttcctcaata cagaactgac ctgggatggt
     3361 atccagcaac cgctgttggg ccataaagtg aatccattca aggcgctgta taaccgcatc
     3421 gatatgaaac aggttgaagc actggtggaa gcatccaaag aggaagtaaa agccgctgcc
     3481 gcgccagtaa ctggcccact ggcagatgat ccgattcagg aaaccatcac ctttgacgac
     3541 tttgctaaag ttgacctgcg cgtggcgctg attgaaaacg cagagtttgt tgaaggttct
     3601 gacaaactgc tgcgcctgac gctggatctc ggcggtgaaa aacgcaatgt cttctccggc
     3661 attcgttctg cttacccgga tccgcaagca ctaattggtc gtcacaccat tatggtggct
     3721 aacctggcac cacgtaaaat gcgcttcggt atctccgaag gcatggtaat ggctgctggt
     3781 cctggtggga aagatatctt cctgctaagc ccggatgccg gtgctaaacc gggtcatcag
     3841 gtgaaataat cccccttcaa ggcgctgcat cgacagcgcc ttttctttat aaattcctaa
     3901 agttgttctc ttgcgatttt gtctctctct aacccgcata aatactggta gcatctgcat
     3961 tcaactggat aaaattacag ggatgcagaa tgagacactt tatctatcag gacgaaaaat
     4021 cacataaatt ctgggcggtg gagcagcaag gcaacgagtt acatgtcagt tggggcaaag
     4081 tcggcactaa cgggcaaaac caggtaaaaa gctttgctga tgctgcggcg gcggaaaaag
     4141 caaagcttaa gctgatcgca gagaaaacga aaaaaggcta tgtggaaaat gcttctgcaa
     4201 acgtgcatat cacccccatc accaaagcca ctcctgaagt tgaaacttct cctgagagta
     4261 aaaaccaacg cccctggctg gctgatgatg cggtcattcg gctaactgac gatattaatc
     4321 gatttgcttt tcctcatcgc tctcgcccca gagagatcaa ttatctgcac aaagatagtc
     4381 aaatatggca gcgcatcgcg aataacacca gggcatatga tcccgccaac aattaccgtt
     4441 cttatccaga aaactggcag caggcttttg ctgagttaca aaggcgaatt ctggataatc
     4501 aacagacagg gagtgcgcaa tctgatgcgg cattgctctg gagtttctgg aattcttact
     4561 ctgcagatga actagtcgat gacttaatca ttcgctgtgg tatggaaagt gccgttgaaa
     4621 ttgcccttct cgctcttcaa ctgagatata aaccagtaaa aggcgcagta accaccatca
     4681 ttccacctaa tcacgaagca gaatcgctac cgagttggca tcaacgccta tgtcatcatc
     4741 tttcactcgc ttcagaagat gagtggcaac gctgtgtaga taaagtactc gcggctatta
     4801 cgacgctatc cccagcacgg caaccttttg cggcgttact gctccctgaa cgcccggata
     4861 ttgccaatgc tatggctcta cgctatgcag atcaaaacgt tccggcgatg acctggttaa
     4921 gactggtagt aaccgatgaa gccgctctga ctgcagccga aaaatacgac tttccaccgt
     4981 tatatcgtga ttttcgcgct tatctggcaa tgttgctggc aaataatggg gtgagcggtg
     5041 taagccggat acttactgag tttgtggaag agcattctga caatcccact tatctcttcg
     5101 agcgcatcag cgagactgaa gatcttgtca aatggttatg gaaaacgaat cacccggatg
     5161 cgattcaaat tctgatcctc ggtgtaattg gcaagaaaaa gcacctggaa tacttaagca
     5221 aagcatgtca aaaacatccc gctgcggcta ttgccgctta tgcaactttg ctggcaatac
     5281 atgaagataa agagtggcat aaagcgcttg tcaaattgat taccatcacg ccagagttag
     5341 tatgcgacgt cattccctgg gttagtgcta aagcggcagg catcttatct gaatgtcgtc
     5401 ctcagtcagt agctgaagaa tgtgagtatg ccactgcgga tatgttgcca gagctgtttg
     5461 tttcgccgcc ctggatgact aaagagaaaa agaaaaattc gccggtgttt gatcttcctg
     5521 tactgccagt tccttctgtt tctgacgtca ctccagagat tgccaaaaaa ctaacccgta
     5581 cataccttgt cacccgcttc cagcaaatag cccaacagca ggcaacaaaa caaatattgt
     5641 ttaccgacct gccgctgatg gaaaaagcaa gctgggagag acacttagtc cccctcacac
     5701 cagaacagca aattctgtgg tgtctgggtt ttgacaaatg gcgggagagc ggagaaaaaa
     5761 tatacgaaaa aattccggcg ccacaaagtg ccgttgatgc cctgctacgc ttcgattttc
     5821 cagcactcaa tgctgaattt gcgcaatacc ataacaatgc ttacaaaagc tggaatctca
     5881 ttgccctctg ttatctacct ggtcaacagg ccatttcttt tcttaaccag atagtcaaag
     5941 aagataacta ctctggtgag ggatatattc tggccatttt tggtagcgcc gccattcctg
     6001 catttatgac ctgcctgcaa cgtgagccac aacggttgtg gtttttcacc ttcttccttg
     6061 gtgtcaatga actggcattg ccaatggcgc aacgacttca gaaaaaaatg tcgtatgaag
     6121 atgcacgaaa ctggctaacg gattacccac gccatgccgc cgccgggcta cttcctgtgg
     6181 cactgggcaa acaaggaaaa gatcgtgatt gtgcccgcca ggcgctccgt ctgctggtga
     6241 atttaaacca gcgagaaacg attgaggaaa tcgcccaagg atacaaccaa cctgatgttt
     6301 tagctgcgct ggcaacatta tttgatagcg agccgctgga agaatatccc gctaaaatcg
     6361 ccccactgcc cggcttttat cagttcacct tatggcgcag accacggctt aaaagtaaca
     6421 accagcctct gccagatgac gctatgcgcc acctcggcat aatgctgagc tttccacgcg
     6481 acattacccc ctacgcaggg cttgcgatca tcaaagaaac cttcacccgc gaatcactgg
     6541 ctgaatttgg ctgggatctg tataccgcct ggacagaagc tggcgcaccc gcaaaagaaa
     6601 actgggcatt tacctcgctg gggattttag gcaatgacga caccgcgcgt aaattaaccc
     6661 cgctcatccg cacctggccg ggtgaatccc agcataaacg cgcggtttac gggctggatg
     6721 tgttagccag tatcggtagc gacattgcgc taatgttgct taatggcatt gcgaaaaaga
     6781 ttaaattcgt tgcattacag gaacatgcct gcgacaaaat caacatggtc gctgaaaatc
     6841 gcgggctgac tatggctgaa ctggaagacc gcctggcacc ggatttagga cttgatagca
     6901 gtggctcgtt gacactggat ttcggcccac gtcaatttac cgttggtttt gacgaaactt
     6961 taaaacccgt ggttcgcgac gcaaacggca aagttctgaa agatttaccc aaaccaaatc
     7021 agagcgatga taaaacgctg gcaactgacg cggttaatct cttcaaacag ttgaaaaaag
     7081 atgtacgcgc catagccagc cagcagattg atcgtctgga gcaggctatg tgccagcgca
     7141 gacgctggac ggcagagcag ttccgcctgt tcctggtgga gcatccgctg gtacgtcact
     7201 taacccgacg gttgttatgg ggggtatata ctgaggaaaa cacgctgatt gcctgcttcc
     7261 gggtcgcaga agacagcact tacagcgatg cgcaggatga gctatttacg ttgccagcag
     7321 gaaacatcgg tattccgcat gtgctggaaa tatctcctga atccgccgct gcatttgggc
     7381 aaatttatac tgattacgaa cagcttcctc cgttccgcca actcgatcgt ggttattacc
     7441 accttgctga taatgaacgt gattcccatg aattgatacg ctggcaagga cgtctttgcc
     7501 aggccgggcg cattgtcggg ctggaacgcc gaggctggca acgtctggaa gaaagcggca
     7561 gcgtttacgc gatgcgcaaa tctactcctt atggcgatct cgaacttgaa acagaacctt
     7621 tttcattaat ttatggtgaa acggggtatg gcgaccagct accggttgaa agcgtaaaaa
     7681 tcacctcgcc tgataatcgc tacggtaaac aatcctcact cactttttcc gcgctcgacg
     7741 acatcaccgc cagcgaactc attaacgaca ttgaatcact gtttgattaa aaggatttag
     7801 caatgaaaac ctatatttat caggacgaaa aatcacataa attctgggca gtagagcagc
     7861 aggaaaatga actgcatatc agttggggca aagtcggcac taacgggcaa agccagataa
     7921 aaagctttgc tgatgcggcg gcggcggaaa aagcggagct gaagttgatt gcggagaaaa
     7981 cgaaaaaggg ctacgcagaa gaagtatccg tgacgacacc gacatctgcc cccgtccagg
     8041 ttatcgcatg ctctgaaaaa gcccctttgc cgcaagacaa accaccattt gctgaagacc
     8101 accttccctg gctggcagac gacgcccaaa tcattttacc gacagaggtc gcaccaacca
     8161 cactaagtca tcgacgctgg cctggcgatt ctgtgccaca ggagaatgaa ttaactctgc
     8221 tacgctccgt cgccgcgcat actcaccggc gttttaaaaa ggtgatcacc ttcgattaca
     8281 gcacctgttc actggactgg cagcaggcga ttacacaagc agtgggcctt atcgactcac
     8341 cgattagcac cgcactacca ccgatggttc tggcggtact ggttgcgctg gaacaaggct
     8401 tcaatcgcaa cgatcacgaa gagttaatgg atcaaattgt ccaggagggt ggtctggaat
     8461 atgccactga ggttgtcatt gcattgcaag ccatccgctt tgactggaat tatgatgccc
     8521 acctcattac gtttaccccg gataacaagc agcctggcta tctgtcacgc tttgcctcgg
     8581 ttgaaatgcg tctgcgtaaa catctctcac tagcaaatga cgatgtatgg cagcgctgcg
     8641 ccgacaaact gattgcagct ctcggaaaca tacccgcctg gcatcagccg ctggttgcgt
     8701 tattgttgcc ggaaaaacaa gatgtgagcc atgagattgc cagacgtctt tgcggacaaa
     8761 aagggttata tgcgctggaa tggctaaaac taacggccgc cgacgagcag gtattggcag
     8821 aactggggaa atactatcca ggacagccag ggcaggtatt tgatgattat tacggcggta
     8881 aaatctggtg cgcaacgata ctcaaggagc aaggtgtcgg tgcgctggcc cgttttgctc
     8941 cttacgctgc tggtgacaca tgtggtgaag tgttgatgca tatcaaccac ccgcaggcac
     9001 ttacactact tatccatgca agcgagcagg gaaagcgttg ccacgatcgc atgacaaaga
     9061 ctttcgttcg ctttccacat gcagcgctgg cggcattagc agaacttctg gcacaaaaag
     9121 atcaaaagcg ttggcgcatg atgctaatga caatgcttat ctcacaacca acattagcag
     9181 agcgagtaat cccctggctc tcaacacccg cagttgccgt actgaaatca tgccagcaac
     9241 aactgacaca gccctcaaac catgccagcg ctgatatgct gcctgccgta ttagtctcac
     9301 cgccctggct ttcgaaaaag aaaaaatctg taatgccggt attggattta acgccattac
     9361 cgctggagtc atgctgcaca ttgaccgaga cggcagaaaa agagattcat gcaaggcatc
     9421 gctggcacgc acatcaaata gacatcggcc aaaaagagga tattcagaac tacctgacac
     9481 gtctgggttt caatcgctgg aataatggcc agtatatgaa agcgtctgat gctgttgtag
     9541 aactctggca gaggggagat tattctgcgc taatcagcga atttaaaacc ttctggcact
     9601 cctatcaacg ggaatggcag ctttacatgt tagccgccct gcctatcgaa aaaacagcgc
     9661 aggcgtggaa tgtcctcagt aaagagccac acgtcggtgt cgagtttgtc atgacgcacc
     9721 tgcaactcgc tgggttacag ggttttattc actccttttc acgctatcca caagaagctc
     9781 tgcctgtagc acaatatttt gcggctatcg aattagcgcc actgatcgcc cgcgccttca
     9841 acaaactcaa aacattacga caagacgccc gtatctggct gctgaaatac ccggaacacg
     9901 ccattaccgg cctgctgcct gcagcgctcg gcaaaaccgg tgaagcacag gacaacgccc
     9961 gtgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attgctgcaa gaaatcgccc
    10021 ggcgttataa ccagccggaa gtaaccgatg cggtgaacgc tctacttgcg ctcgatccct
    10081 tagataatca cccgacaaaa atccccactc ttccgacctt ttatcagcca tcgctctgga
    10141 cgcgcccgtt attaaaagca aacgcccaat cactgcctga tagcgcactc ctccacctcg
    10201 gtgaaatgct ccgcttccct caggaagagg cactgtatcc aggattattg caggtgaaag
    10261 atgcctgtac caccgactca ctggctgaat ttgcctggga tctgtttacc gcctggcaga
    10321 ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt ctcggtaacg
    10381 atgacaccgc ccgcaaactg acgccattaa tccgtgcctg gcctggcgaa tcccagcata
    10441 aacgtgccac cgttgggttg gatatcctcg ccgctatcgg tagtgatatc gccctgatgc
    10501 agcttaacgg catcgcccag aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa
    10561 aaatagccaa cattgccgag agccgcgaac tcacggtggc ggagcttgaa gatcggttag
    10621 caccggatct cggtctggat gataacggtt cgttgctgct ggatttcggc ccacgccagt
    10681 tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgacgcttcc ggcagtcgtc
    10741 tgaaagacct gcccaaacca aacaaaagcg atgatgaatc acaggcgaac aatgctgtta
    10801 accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc cgcccagcag gtagcaaggc
    10861 tggaatccgc catgtgcctg cgtcgccgct ggtcaccgga aaactttcag ctcttcctgg
    10921 ttgagcatcc gctggtccgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg
    10981 aaaaccagct actggcttgc tttcgcgtag cggaagataa cagctacagc accgctgacg
    11041 atgatctttt caccctgccg gaaggcgata tctctattgg tattcctcac gttctggaaa
    11101 tatcaccgac ggatgctgcc gccttcgggc agctttttgc cgactacgaa ctgttaccac
    11161 cgttccgcca gcttgatcgt aacagttacg cgctgacaga agccgagcgc aatgccagcg
    11221 aactgacacg ctgggcaggc agaaaatgcc cgagtggtcg ggtaatgggg ctggcaaata
    11281 aaggctggat gcgtggtgaa ccgcaggacg gtggctggat cggctggatg atcaaacctc
    11341 tgggccgctg gtcgttaatc atggaaatcg atgaaggttt tgcggtcggc atgtcgccag
    11401 ccgaactcag tgccgagcaa ctcttaagca agctgtggct atgggaaggc aaagcggaaa
    11461 gctatggctg ggggagtaat tcaacacagg aagcgcagtt ctccgtactc gatgccatca
    11521 ccgccagcga gctaattaac gatattgaag ccctgtttga ataaggaaag agcatggaca
    11581 aggaattacc gtggctggcg gataacgccc aactggaact gaaatataaa aaaggcaaaa
    11641 caccactcag ccatcgcaat tggccaggcg aaccggtacc tgttatcact gaaaacatca
    11701 ttcagacact gggtgatgaa ttgctacaaa aagcagagaa gaaaaaaaac attgtctggc
    11761 gttatgaaaa cttttcactg gagtggcagt ccgccatcac gcaggccatc aacttgatcg
    11821 gcgaacacaa accctcaatc acggcccgga caatggcggc gctagcctgt atcgcgcaaa
    11881 atgatagcca acagttgctc gacgaaatcg tccaacaaga ggggctggaa tatgcaactg
    11941 aggtggtgat tgcacgccag tttattgtac ggtgttatga gagtgatcct ctggtagtaa
    12001 cattgcaata tcagaacgag gactatggct atggttatcg ctcagaaacc tataacgaat
    12061 tcgatctccg attgcgtaag catctctctc tggcagagga aagctgctgg cagcgttgcg
    12121 ccgacaaact cattgccgca ctgccaggaa taaccaaagt tcgccgccct tttattgcgc
    12181 tgaacctccc ggaaaaaccc gaaatcgcca acgagctggt aagccttgaa tgttcgcaaa
    12241 ctcattttcg ctcaaaagag tggttaaaag ttgttgctga tgaccctaag gcggtgaaag
    12301 aactcgcacg ctactggagc caggatatat ttagcgatcg agaagccagc tacatgtcgc
    12361 atgaaaacca cttcggctac gcggcctgcg ctgccctttt gcgcgaacaa ggactggcag
    12421 ccgttccgcg cctcgcgatg tatgcacata aagaagattg cggcagtctg ctggtacaaa
    12481 ttaaccatcc gcaagtcatc cgcaccttgc tactggtggc tgataaaaac aaacccagcc
    12541 tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc gacgctcgcc gcactggcag
    12601 agctgctggc gttaaaagcc ccccctgcgc gccctggtta tcccatcatc gaagacaaaa
    12661 agctgcctgc acagcaaaaa gcacgtgatg aatactggca tacgctgtta caaacgctga
    12721 tggcatcgca gccacaactg gcagaagagg tgatgccgtg cttatgtaca caagcccagg
    12781 cagtggtgaa tggttattta tcggcatcgc caaaacttgc tttcgagagt actcatagca
    12841 acgacctgcc agaaatactt gtttctccac cgtggcgagg taagaaaaaa acggcattgc
    12901 tgcgccttga tttagtgcct cttgaattag cgcccaaagc ccgctggcaa ccaggcgaac
    12961 gagagagact tgccgccaca gagtctgccc gttatttcag cacgggatct tttacggaac
    13021 gcatggaacg aaaaagtggt cgagttgtac tacaggagct aggttttggg gatgatgtat
    13081 ggctgttccg gaattatata ctgcccggca aactggatgc ggcgcgcaaa tcattagtgg
    13141 gtcagtggca ttactccccg cgcagagtag aggaaatcaa caatggctgg cactccacgg
    13201 aagcaaaatc agccgaacag gcgcttcgtt ccggcgacgt tgaagcgtta attaacacct
    13261 gggaaaatga tagctactcg cattatcgtc aggaaaagag tgtctggaac ctgtatttat
    13321 tggcgcagtt gccacgtgag atggctttga ctttctggct gcgtatcaat gagaaaaagc
    13381 atctattcgc gggtgaggac tattttctca gtatcctcgg gttggataca ttacctggcc
    13441 tgttattggc tttttcacat catccaaaag aaacatttcc gttaatttta aatttcggcg
    13501 caacagaact ggccctgccc gttgcccgcg tctggcgacg tttcgcggcg cagcgtgatc
    13561 tggctcgcca gtggatttta caatggccgg aacatacggc tactgcactt atccctcttg
    13621 tctttacaaa acccagcgat aatagcgaag ctgcattact tgccctgcgt ttactgtatg
    13681 aacaagggca tggcgaatta ctgcaaaccg tggcaaaccg ctggcagcgt acagatgtat
    13741 ggcctgccct ggagcaatta cttaaactgg gtccaataga aatttacccg gcacgtattc
    13801 caaaagctcc tgatttctgg catccgggaa tgtggtccag gccgcgcctt atcactaata
    13861 atcagcctgt taccgatgac gctctggaaa ttatcggcga aatgctgcgc tttacccagg
    13921 gcggacgttt ttatggcgga ctggaacagc tgaaaacgtt ttgccagccg caaacgctgg
    13981 cagcttttgc ctgggatctt ttcaccgcgt ggcaacaagc tggtgccccc gcaaaagaca
    14041 actgggcatt tctggcgtta agtctctttg gtgacgaaac cacggcacga gatctgacga
    14101 cacagatcct cgcctggcca caagaaggca aatctgcccg tgctgtcagt ggcctgaaca
    14161 tccttaccct gatgaataat gatatggcgc tgatacagct gcaccatata tcccaacggg
    14221 cgaaatcccg ctccttacgt gataacgcgg cggaatttct tcaggtggtc gcagaaaatc
    14281 gcgggctaag ccaggaagag ttagcggaca gattagtccc aacattgggc cttgatgatc
    14341 cgcaggcgtt gagttttgat tttggtcccc ggcagtttac cgttcgcttc gatgaaaacc
    14401 tcaacccggt tatctttgat cagcaaaacg ttcgccagaa aagtgttccc cggttgcgcg
    14461 ccgatgacga tcaactgaaa gcgcccgagg cactggcccg actaaaaggg ctaaaaaaag
    14521 atgccactca ggtgagcaaa aacctgctcc cacgtcttga agcagcccta cgtatcaccc
    14581 gccgctggtc gctggcagat tttcattctc tgtttgttaa tcatcccttt acccgcctgg
    14641 ttacccagcg attaatatgg ggggtttatc cggcaaatga accgcgtcgt ttactcaacg
    14701 cctttcgtgt ggccgcagag ggggagttct gcaatgcaca agatgagccc attgacctgc
    14761 ctgcggatgc tctgattggc attgcccacc cgttagaaat ggcagcagaa atgcgcagtg
    14821 aatttgcaca gctttttgcc gattacgaaa ttatgccgcc ttttcgccag ttggcgcggc
    14881 gcacggtgct gctcacacct gacgagtcag ccagtaacag cctgactcgc tgggaaggta
    14941 aatccgctac cgttgggcaa cttatgggaa tgcgatacaa aggctgggag tcaggctatg
    15001 aggacgcatt tgtctatgac ttaggtgcgt accggctggt ccttaagttt tcacccggtt
    15061 ttaaccacta caatgttgat agcaaagcgc taatgagctt ccgttctctt cgggtttacc
    15121 gtgacaataa atccgtcact tttgccgaac ttgatgtgtt tgatttgagt gaggcattaa
    15181 gcgcacctga cgtcattttc cattaaaaca cagcgggcag tgatgcaact gcccgttatc
    15241 aacacgacct accagcgggg gaagcgatga tcgtgcaaaa agagctggtt gctatttacg
    15301 attatgagat ccctgtacct gaagagccgt tttccttcag acttgaaatc cataaatgct
    15361 ctgaattatt tacaggttcc gtctatcgac tggagcgatt ccggctacat ccaacatttc
    15421 atcaacgtga tcgagaagat gccgaccact aataaatgat gcgttgattt atataagaga
    15481 tgagtttatt gatgagcgga aattacgagg tgaatcacat gaaacagtaa tagcaatttt
    15541 taattgtgaa ctacagaata tattcaacca agaaatagaa taatatactc tagataattc
    15601 aagtcgcagg aaggcgataa gcgagtgagt ccccaggagc ttactaaagt aagtgacagg
    15661 ggtgaacgaa cgcagtcgca gcacatgcaa cttgaagtat ggcgagtata gctttattaa
    15721 agggggaaac ggtattcccc cttagatgcg tttcaatttg cccgttactt caacaaatca
    15781 atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt taacttttgt
    15841 gtcagcgtgt ccatccgctc tccttttaag gagcgcaaca ccagcacctt acccttacac
    15901 tgcctgtcga tactggttta cgtatgttgg accacgatgt tgcactccgg atcaggcacc
    15961 acctgcagca ccacccgtcc ggtagccagg acttgaaatt ggttcgatcc ggcgcaacct
    16021 cccaacagtc aggattataa atctcattat cttaattgca acggggtcca gcggtggtta
    16081 taattcgttc gttggaacac gatgttgcac aggctgcggt gttaggcctg aaagtaataa
    16141 ataaaagaac ctgcctcacc agcaggtttt tttatttttt gcgatctgct ttccagatat
    16201 tttccgctca aacaactaat gcgccaaaca tttattgcgc gtaaaatatc gtttatttca
    16261 ttaatacatt tcagggatga atatatgtca ccgcagaata atcacctgca gcgtccgcct
    16321 gctgctgtgt tatacgccgt tgaactggca aaattaaaac aaaatgataa cgcaccttgc
    16381 ccgcccggtt ggcagttaag tttgcctgcg gcccgtgctt ttattcttgg cgacgaagcg
    16441 caaaatattc gccgtaaagt ggtgattagt ccctctgctg tcgaacgcat gttagtcact
    16501 cttgccaccg ggcgcggttt gatgttggtg ggggaaccgg gtaccgcaaa atctctcctg
    16561 tctgaattac tggcaaccgc tattagcggc gatgccgggt taaccatcca gggcggcgca
    16621 tctaccaccg aagatcaaat caagtacggc tggaactacg ccctgcttat caatcatggt
    16681 ccgtcaacgg aagcgctggt ccctgccccg ctttacctgg gaatgcgcga cggcaaaatc
    16741 gtccgttttg aagagataac gcgtactcca ctggaagtac aagactgttt attaggtatg
    16801 ctttctgaca gggttatgac ggtgccggaa ctcactggtg aagccagtca actctatgct
    16861 cgggaaggct ttaatattat tgccactgcc aatacgcgcg atcgcggcgt caatgaaatg
    16921 agtgccgcgc taaagcgtcg cttcgatttt gaaactgtct ttccgattat ggatttcgcc
    16981 caggagctgg agcttgtcgc cagtgcttcg gcgcgtttgc tggcccatag cggcattccg
    17041 cataaagtgc ctgacgcggt actggagtta ctggtccgca cctttcgcga tctgcgtgcc
    17101 aacggcgaaa agaaaacgtc aatggataca ctgacagcga ttatgtccac cgctgaagcc
    17161 gtgaacgtgg cccacgcggt gggcgtccgc gcctggtttt tagcgaatcg agcaggtgag
    17221 ccagccgatc tggtggactg tattgccgga accatcgtca aagataacga agaagaccgc
    17281 gcgcgcctgc gccgttactt cgaacaacgt gttgccacac ataaagaagc tcactggcag
    17341 gcttattatc aagctcgcca ccgcctgccg tgaggaaaga tgcatgagcg agccgttaat
    17401 tgtcggcatc cggcatcata gcccggcctg cgcccggctg gtgaaatcgt taatcgaaag
    17461 ccagcggcca cgatacgtat tgattgaagg cccggctgat tttaatgacc gggtagacga
    17521 actgttttta gcccaccagc ttccggtagc tatttacagt tattgccagt atcaggacgg
    17581 tgcggccccc gggcgtggtg cctggacgcc atttgctgaa ttttcgccgg agtggcaggc
    17641 gctacaagcc gcacgtcgta ttcaggcaca aacttacttc atcgatttgc cttgctgggc
    17701 gcagagtgaa gaagaggacg attcgcctga tacacaagat gaaagccaga ccttactgct
    17761 gcgtgccacc cgcatggata acagcgatac cctgtgggat cacttattcg aagatgaaag
    17821 ccagcaaact gcattaccct ctacgctggc gcactatttt gcccaactgc ggggcgattc
    17881 ccccggagat gctctcaatc gtcagcgcga agcctttatg gctcgctgga ttacatgggc
    17941 gatgcagcaa aacaatggcg acgtgttagt cgtctgcggc ggctggcacg ctcctgcact
    18001 ggcaaaaatg tggcgcaaat gcccgcagga aatgaacaag ccagaattgt cctcgctggc
    18061 agatggcgtt acaggttgtt atctcacacc ctacagtgaa aagcgccttg atgtgctggc
    18121 aggatacctt tccggaatgc ctgccccggt ctggcaaaac tggtgctggc agtgtggctt
    18181 acagcaggca ggtgaacaac tgctgaaaac ggttcttacc cgtttgcgcc agcacaaatt
    18241 gcccgcttct accgctgata tggctgctgc tcatctgcat gcgatggcac tggcacagtt
    18301 gcgcggccat acactacctc tacgcactga ctggctggat gccatagctg gctcgctgat
    18361 taaagaagcc ctgaatgcgc cgttgccgtg gagctatcgc ggagttattc atcccgatac
    18421 cgatccgatt ttgctaacgg tgatagacac attagcgggt gacggattcg gtaaactttc
    18481 cccttctacg ccacaaccgc ctctgccaaa agatgtcacc tgcgaactgg aacgtaccgg
    18541 aatctctctt ccggcggagc ttaccttaaa tcgctttacc cccgatggac tggcgcaaag
    18601 tcaggtgtta catcggttgg caatactgga gatcccaggg gttgtgcgcc agcagggaag
    18661 tacgctgaca cttgcaggca acggtgaaga atgctggaaa ttaacccgcc cgcttagcca
    18721 gcatgcggca ttgattgagg ccgcctgctt tggtgccaca ctccaggaag ccgcacgcca
    18781 taaattagaa gccgatatgc tggacgcggg tggaatcggc tgtatcacca catgtcttag
    18841 ccaggcagcg ttagcgggtc tggcgtcttt cagtcagcaa ttactggaac aactcacatt
    18901 attaattgcc caggaaaatc aattcgccga aatgggccag gcgctggaag tgctatatgc
    18961 cttatggcgg ctggatgaaa ttagcggtat gcaaggcgcg cagatattac aaacgacatt
    19021 atgcgcggcc atcgatcgca cgctgtggct atgtgaatcc aacggcagac cggatgaaaa
    19081 ggagtttcac gctcacctgc atagctggca ggcgctttgc catatcctgc gcgatctaca
    19141 tagcggcgtt aatttacccg gcgtttcgct ttctgcggcg gtagccttac tggagcgccg
    19201 cagtcaggcg attcatgccc cggcgctgga tcgcggcgcg actcttggcg cactgatgcg
    19261 tctggaacat cccaacgcca gtgccgaagc ggcgctgacg atgctggcgc agttatcccc
    19321 ggcacaatcc ggcgaggcgc tgcacggttt gctggcatta gcccgtcatc aactggcctg
    19381 tcagccggta tttatcgccg gtttcagcag tcatttaaat caactgagtg atgccgattt
    19441 tatcaatgcc ctgcccgatt tacgcgcggc gatggcctgg ctaccaccac gagaacgcgg
    19501 gacgctggcg catcaggtgc ttgagcatta ccagctcaca cagcttcccg tttcagcgct
    19561 gcaaatgctg ttgcattgtc caccacaagc cattgcacat catcaacaac tcgaacagca
    19621 ggcactggca tcgctgcaac actggggagt tttccatgtc tgaactgaac gatcttctga
    19681 ccacccgtga gctacaacgc tggcgattaa ttcttggcga agcggcagaa acgacccttt
    19741 gtgggctgga tgacaacgcc cggcagatag accacgcgct ggagtggctg tatgggcgcg
    19801 atcctgaacg gctccagcgt ggtgaacgtt acggtggatt aggtcgttca aatctcacca
    19861 cccctgagtg gattaacagc attcacacgc tgtttccgca acaggtgatt gagcggctgg
    19921 aaagcgatgc cgtgctgcgc tacggcattg aagatgtggt gacgaatctc gacgtgctgg
    19981 aacgtatgca gccttctgaa agcctgctac gcgccgtttt gcacaccaaa catctgatga
    20041 atcccgaagt actggctgcc gcccgccgga tagtgcacca ggttgtggaa gaaattatgg
    20101 ctcgactggc aaaggaagtt cgtcaggctt tttctggagt acgcgatcgc cgtcgccgct
    20161 catttatttc actggcgcga aactttgatt tcaaaagtac tctgcgcgcc aacctgcaac
    20221 actggcaccc gcaacacggc aagttgtata tcgaatcccc ccgctttaac agccgcataa
    20281 aacgctatag cgaacaatgg caactggtcc tactggttga tcaaagcgga tcgatggttg
    20341 attcggtgat ccactccgca gtgatggcgg cctgtttatg gcagttaccc ggcattcgta
    20401 cccatctggt cgcttttgac accagcgtgg ttgatctcac ggcagacgtt gccgatccgg
    20461 tagagctatt aatgaaagtg caattaggcg gcgggaccaa tatcgccagt gctgtggaat
    20521 atggtcggca acttattgaa caaccagcga aaagcgtcat tatcctcgtg agcgattttt
    20581 acgaaggggg gtcatcatca ttgctgacgc atcaggtgaa aaagtgtgtc cagagcggca
    20641 tcaaagtgct gggactggcg gcgctcgata gcagcgcaac accttgctat gaccacgata
    20701 cggcccaggc gctggttaat gtcggcgcac aaatagccgc aatgacaccg ggcgagctgg
    20761 cttcctggct tgcggagaat cttcagtcat gaattcacta cgtccggaat tattagaact
    20821 gacgccgcag gctctgacgg cgttaagcaa tgccggtttt gttaagcgca gccttaagga
    20881 actggaaaat ggcaacgtcc cggaaatcag tcatgagaac ggcgctttaa tcgccacctt
    20941 cagtgacggt gtccgtaccc agctggcgaa cggccaggca ctgaaagagg ctcagtgcac
    21001 ctgcggggcc agcggcatgt gccgtcatcg cgtgatgctg gtgttaagtt atcaacgact
    21061 ttgtgccacc gcccagccta cggaaaaaaa agaagaagag tgggatccag caatctggct
    21121 gaaagaactg gctaacctgc ccgatgccac ccgcaaacgc gcgcaggcgc tggtcgctaa
    21181 aggcatcacc attgagttgt tctgtgcgcc tggcgaaatt ccctctgccc gattaccgat
    21241 gagcgatgtg cgtttttatt cccgcagcag cattcgcttc gcacgctgtg attgcattga
    21301 aggcacactt tgcgaacatg tcgtactggc tgtgcaggcc ttcgtcgagg ccaaaactca
    21361 gcaagcggaa tttactcatt taatctggca gatgcgcagt gaacacgtga catcatctga
    21421 tgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcagc aattaagcca
    21481 ggcattatgg ctgggcggga taagtcaacc gccaatccat tacgaggccg ctttcagtcg
    21541 cgcgcagcaa gcggcggaac gctgcaactg gcgatgggtg agtgaatcgc tacggcaatt
    21601 gcgcgccagt gttgatgcct tccacgcccg tgccagccac taccatgccg gagaatgctt
    21661 acgtcagctt gcggcattaa acagtcgatt aaattgcgta caagagatgg cgcgacgcga
    21721 cagtatcggt gaagttcctc ccatgccgtg gcgcacggtc gttggcgctg gcattgccgg
    21781 agaagcaaaa cttgatcatc tgcggctggt gtctttaggt atgcgttgct ggcaggatat
    21841 tgagcagtat ggtttacgca tctggtttac cgatcccgac acaggaagta ttttgcatct
    21901 ttcgcgtagt tggcagagaa gcgaacagga aaactcaccg gcagcgacgc gtcggctgtt
    21961 tagttttcag gctggcgcac tggcaggtgg gcaaattgtt tcacaagctg caaaacgcag
    22021 tgccgatggc gagctgctgt tagcgacccg taaccgcttg agtagtgttg ttccactgtc
    22081 gccagacgcc tggcaaatgt tgagcgcgcc gttgcgccag ccgggcattg tggctttgcg
    22141 ggaatattta cgccagcgtc cccccgcctg catacgacca ctcaaccagg tcgataactt
    22201 atttattctg ccggtagcgg agtgtatttc gctcggttgg gatagcagcc gccagacact
    22261 ggatgcgcag gtaatcagcg gcgaaggaga agataatgtg ctgacgttat cccttccggc
    22321 atctgcctgt tctccttttg ccgttgaacg catggcggcg cttttgcaac aaacagacga
    22381 ccccgtgagt ctggtttctg gctttgtcag ttttgtagag gggcaattga cactggaacc
    22441 acgggtgatg atgacaaaaa cccgcgcctg ggcgctggac gcagaaactg cgcctgtggc
    22501 accgctacct tctgccagcg ttctgcctgt gccgtctacc gctcatcagt tgctgatgcg
    22561 ctgccaggcg ttacttattc aactgctcca taacggctgg cgctatcagg aacagagtgc
    22621 tattggtgag gcagagttgc tggcgaatga cctcaccgcg gtgggttttt atcggctggc
    22681 acatgtgttg ggacaatttc gtaatacaga aagcgaggca cgggtagaag caatgaataa
    22741 cggtgttttg ctttgcgaac aattattccc catgcttcag caacaaggat gaaatagtgc
    22801 tttttattaa gagttctact cctgttccgg actgctcacg ccacagtatt aggcatatcc
    22861 tatatagccc ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga
    22921 aggctttcaa taagctgttt tcccttgttg ttgcatctgt tctggttttc tctcttgctg
    22981 gctgcggtga caaagaagaa tcaaagaaat tcagcgccaa tctgaacggc actgaaattg
    23041 ccattaccta tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc
    23101 aatttgcctc cattggtgca accaccaaag aagatgccgc caggacactt gagccgttaa
    23161 gcgccaaata caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg
    23221 cgcaggaaaa cgtgactatt gatatggaaa aagtggattt taaagccctg cagggtattt
    23281 caggaatcaa tgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac
    23341 tggtgatgaa agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc
    23401 atggccgcca tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag
    23461 aaaataacgt aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcacgcat
    23521 catcgccgta atttccggca tcgaaacgcg gaactgctgt tcggtgagga ttgccagaat
    23581 gtcatcggtt ttaagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat
    23641 gcgacgttcc ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag
    23701 gccattgagg aatgaagaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc
    23761 tttacgtagc cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat
    23821 acgcaccagg tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt
    23881 acttagcatg aggccttcag gtgttgatga ggcaaaaagc cattttagca gtcttttaca
    23941 ggccaatcgc ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat
    24001 tacgcaaaat caactcggcc tggccgttat cttccagacg gatctcctgt aaatgcgcga
    24061 ggttaaccag atactgacga tggcagcgca gtaatggtgt acgactttcc agggtacgta
    24121 atgtcagttc ggtaaagccc tctttccctt cgtgactggt aacgtagaca ccgctcatcc
    24181 gactgctgac aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac
    24241 aagggataaa tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc
    24301 gctcctgacg caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca
    24361 gataatcaaa ggcatgttct tcaaaggctt taatggcgta ttcgtcaaac gcggtgagaa
    24421 aaacaatata cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc
    24481 gcggcatctg gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc
    24541 cttccacggc gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata
    24601 cgcgcaggtt ctcccgtgca agcggttcat catcgacaat taagacttta atcatgcctc
    24661 gtccctccat ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct
    24721 tatcccatag tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag
    24781 cccactggca ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa
    24841 atgttgcccc tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt
    24901 cccatgttta atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg
    24961 ggataattct tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc
    25021 aatttgcaga taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg
    25081 ccgctttaag tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc
    25141 gctgtcgcgg cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatggggatt
    25201 cacctgggcg tgaagcagtt tgatctctga ctgggtgagc atcgcttttt gccgctcata
    25261 ttgcccggcg aggatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt
    25321 gattgaactg aataaacggt ttttggcttc atacaatttg atggtaccca tcactcgctg
    25381 attttcacca cgcaacggaa tgaccagcgt cgaccccagt ttgcattgcg gatgcaaaga
    25441 gcaacggtaa ggcacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt
    25501 taaggtataa gtcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt
    25561 aaaggccagc aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata
    25621 cagcacctgt gccactttca tgctgttcac ttcgttaaac ccctggcgca aaatgccttc
    25681 cgtcgaggcc gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat
    25741 cgcgcgttta tcgagcaata tacgcataaa caacgccgcg ccgacggtat tggtgaccat
    25801 cattggcgca gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc
    25861 caggatgatc aacatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg
    25921 gttaaagact ttatcagtgc gcccgcgacg gatcaggatg ctgtgaacca ggccgccgag
    25981 caatccttca acgatggtcg agatcatgca acttagcgcg gtcatgcccc ccatcgaata
    26041 tcgatgtaac ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc
    26101 gcccattacc gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa
    26161 ccaggtgccc atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag
    26221 acgaaccgtg acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat
    26281 gactaaaaaa acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc
    26341 cgcaaaccac acttcccttt aaaatgcgta acatacattg cttgcgttta actttctttg
    26401 aactcttgca gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat
    26461 aaaatcaccc tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc
    26521 taaagttaaa ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg
    26581 aagtggcgtt gctttgtgat attaaccctg tcacgttacg cgcgtggcag aggcgttacg
    26641 gattgctgaa accgcaacgg acagacggcg gccatcggct gttcaacgat gccgatattg
    26701 accggatccg cgagatcaaa cgctggatcg acaacggcgt gcaggtcagc aaagtaaaaa
    26761 tgctgctcag taatgaaaat gttgatgtgc agaacggctg gcgcgatcag caagaaacat
    26821 tactgactta cctgcaaagc ggcaatctgc atagcctgcg aacgtggatc aaagagcgcg
    26881 gtcaggatta ccccgcccag acactcacca cacatctgtt tattcctctg cgccgacggc
    26941 ttcagtgcca acaaccgact ctccagacgc tgctggcgat cctcgacggc gtactgatca
    27001 actacatctc tatttgcctg gcttcggcgc gcaaaaaaca gggtaaagat gcactggtgg
    27061 ttggctggaa tattcaggat accactcgtc tgtggctgga gggctggatt gccagtcaac
    27121 aaggatggcg cattgatgtc ctcgcccact cgctcaatca actacgccct gaactgttcg
    27181 aaggccgtac attgctggtg tggtgcggtg ataatcgaac ctccgcccaa cagcagcaac
    27241 tcaccagttg gcaagaacaa ggccatgata ttttcccact cggcatttaa tgattcgtta
    27301 acaaatgcgc tttactgtac aatcctttcg ttaacataag gagtgcatta tgcgcatagc
    27361 taaaattggg gtcatcgccc tgttcctgtt tatggcgtta ggcggaattg gtggcgtcat
    27421 gctcgcaggt tataccttta ttttgcgtgc tggctgaacg cctgcaccag cctttcaaac
    27481 aggcggtctg cgatgatcgc cgccagtgcc accagtaacg ctccctggat cacatacgcg
    27541 gtattaaatc cgctaagtcc gatgatgatg ggcgtaccca gcgtgctggc ccctaccgtt
    27601 gaggcgatcg tcgccgtacc aatgttgata atcaccgaag ttcgcacgcc agccagaatc
    27661 accggagccg ccagcggtag ctcgacctta cgcagtcgct gaccacgact cattcccata
    27721 cctttcgcaa cttctgtcac gctggcatca atcgctccca gcccggcaag tgtcgcctgc
    27781 aggacgggca gcacgccgta aaggatcaag gcgataatcg ctggtttcag accaaagccg
    27841 atcaccggaa cggcaatcgc cagcaccgcg acgggcggaa aagtctgccc aacggcggca
    27901 atagtttcca ccagtgggcg aaattccgca ccccacgggc gagtgacagc aattccggca
    27961 ccagtaccaa tgatcaccgc aaacaaactc gaaattccca ccagccagaa atgagccagt
    28021 gccagagctg caaaactttc ttgctgataa acgggtcgtg gcagttgtgg gaacaaggca
    28081 gcaaacagcg gctggctata aggtagccag aaaatcagtg ccacaaacaa agcaatgagc
    28141 cagaacagcg gatcgcgcaa catcttcata cgcttacgcc tcctccagca gatcctgaaa
    28201 atgcagcgtg ccgcaaggct ggccctgcgt gttcaccacc ggcagcacct cgcatccccg
    28261 cgcgacaaac agagaaagcg catcgcgtag cgtcatcctt tcttccagtg cctcaccttc
    28321 agcccgttct tcacggcgca cgtaatctgc cacgctacgt aacgaaagca ggcgtacacc
    28381 cagctcacta cgaccaaaaa actggcggac aaaatcattc gccggatgag tcagcatcgc
    28441 cagaggattg ccctgctgca cgacttcacc gtgatccatc aataccagat gttctgccag
    28501 ccgtagcgcc tcatcaatat catgagtgac cagcacaatg gtacgcccct gcaaacggtg
    28561 aatgcgcgtc atctcttgtt gcaacgcgcc gcgcgttacc gggtccagcg cgccaaaagg
    28621 ttcatccatc agtaagactt gcggatcggc agccagtgcg cgcgccactc ccacacgttg
    28681 ctgctgacca ccggaaagct gatgcggata acgctcacgc aaatttggct ccagccccag
    28741 tagcgccatt aattcgtcga tacgatcgtc gatccgcgcc cgtgaccatt tttgcaattg
    28801 cggcacggtg gcgatgtttt gtgccacgct ccagtgggga aacaggccaa tagattgaat
    28861 ggcatagccc atccggcggc gcaactccag tactggcagc gagcgaattt cttctccggc
    28921 aaagcggatc accccgctgt catgctccac caggcggtta atcattttca gagtggtgga
    28981 tttaccggag cctgatgtac caatcagcac cgaaaaactc ccttcctgaa aattgagatt
    29041 gagatcgtta acggcttttt gtgcgccgaa cagtttgctg acatggctaa attcaatcat
    29101 tacgtttcac cttcagcagt gcgataagca aatcgaacag cgcgtcggta agcaccgcca
    29161 gaacaattac cgggatcacc cccagcaaca ctaaatcaat ggcactgctt agcagcccct
    29221 ggaaaaccag cgcaccaaaa ccgcctgcgc cgattaacgc cgcaatcacc gccatgccaa
    29281 cggtttgcac catcaccacc cgcaggctgc gcagaaatac cggtaacgcc agcggtaact
    29341 gaacatgcag gaaacgctgc accccgctca tccccatcgc tctggcgctc tccagcacat
    29401 cgcgcgggat ctggttcaag ccgactacta cgccgcgcac cagcggcagc aaggcataga
    29461 gcaccagcgc aataagtgcg ggtgtcattc cggttcctgc tatgccgagc gttcccagcc
    29521 acggaaaggc cgtaaccagc gcggcaagcg gcgcaatcag caggccaaag agcgctaccg
    29581 aaggcacggt ctgaatgaca ttcagcagag aaaaaatcgc cccctgccgc gcggtggaaa
    29641 agtagcacca gatgcccaac ggcacaccaa tcactaacgc aggcagcacc gcaccaaaca
    29701 gcaacgtcag atgttgtgcc agagcgtcgt caaacacatc ctgacggttg gcgtattctt
    29761 tcattaacga gagatcgtta agcgtgccgg agtacagcaa ccacagcgga ataatggcaa
    29821 tctgcatatg caacaaccag cgccacagcg gatgcgtgga gattcggcgg atggcatcgc
    29881 tacaggccag cagtgccagc gccgcagcca gccagaaacc actgccgagg ctggtacgtg
    29941 ccagcgcact gccattttgc gccagttgtg tcgccgcctt tccagctccc cacaccagca
    30001 atacgaagac gaattgcgcc agaatgagtg caaaaatgct gcctttttta gcgggaataa
    30061 aacaggctgt cagccaggcg caaccaacgc ccaccagcat ccagagagtt tgcggccaca
    30121 gctgccagag atgacgcccc tcacccgaaa ctaaacgatt aggcgcgtaa ctgataaacg
    30181 gcagcgccgc tgcgattgcc gtcagcaaca gcagcagcgc cagaacagga ttaatacgta
    30241 gataagtcac gggaaattac ttcgtccacc ctttttgttt caggtagtcg gcagccactt
    30301 ttttggcatc cagtccttcc acagcaatgc tggcattcag ttgctgcaat gttttttcat
    30361 cgaggctggc gaagactggc tgtagccact gtgccatttg tggatactct ttcagcaccg
    30421 actcacgcac cactggtgca ggcgcgtaga taggttgcac gccttgcgga tcgcttaagg
    30481 tttgcagccc cagcgccgcg accgggccgt cagtgccgta agccattgcg gcattaacgc
    30541 cagaggtttg ctgggcagcg gctttgatcg ttaccgccgt gtcgccacca gccagtgaca
    30601 gcaactgatc ctgaccgagc ttaaagccat aggctttttc aaacgcgggt aaggcatcgg
    30661 cgcgttcaat aaactctgcc gaggctgcca gtttgaaggt gccgccctct tgcagataac
    30721 gactcaggtc ggcaagcgaa gtgagcttgt ttttctctgc cacatcctga cgcacggcga
    30781 tggtccaggt gttatttgca ggtgcgggcg tcagccagat taacttgttt tgctctgcat
    30841 cgagtttttt gactttctcg taaccttgct gcacgttttt ccacgctgca tcgttttcat
    30901 ctttaaagaa gaacgcccca ttgccggtat attccggata gatatccagc tcgccagaag
    30961 tgatcgcgcc gcgcaccaca ggagtcgttc caagttgcac tttatttacc gtaggaacgc
    31021 cgtgactttc cagcacctgc aaaatgatat tgccgagcag cgcgccttcc gtatcgattt
    31081 ttgaaccaac cttaacgggg gaagctgcct gtagcggcag gctcacggct gccaacaaaa
    31141 ccagtgaacc tgcccagacc tttgagagtg gcatgatgct ttcctcattc ttttactgtt
    31201 gttttcagcg aattaagaga aaagcatagt tgataatgac agggttagcc tgtctgggtg
    31261 gattcagttg cagaatcaga taaatactta aaaggcatat tttgcccggc gtttcttgcc
    31321 ggatgcggcg taaacgcctt atccggccta caaacatcga aaattacaac aactcaaact
    31381 cggctttctt aacgcgtgcg gaatcagtgc cgataaagac attgaacttg ccaggctcgg
    31441 cgtcatattt catctgttga ttccagaact tcagcgcctc aatatcaatc gggaagctga
    31501 cggtctgagt ttcgcccggt ttcagagtga ttttctcaaa gcctttcagc tgcttcaccg
    31561 ggcgactcat ggaagccgtc acatcctgca agtacatctg tactaccgtg gccccttcgc
    31621 gtttaccggt gttcgtcacc tgcacgctgg ccgtcacttt gccgtcacgc ttcatggtcg
    31681 gcgcagaaag tttcacatca gagacggtga aagtggtgta gctcagccca tagccgaacg
    31741 gataaagcgc cccgttagct tcatcaaaat aacgcgaagt gtatttgttc ggcttgtcgg
    31801 cattatacgg acgcccggta ttcagatggc tgtagtacac cgggatctgc ccgacagaac
    31861 gcgggaagga catcggcagc ttgccggacg ggttataatc gccaaacaat acatcggcaa
    31921 ttgcattacc gccttcagtc cccgcaaacc aggtttccag aatcgcatca gcctgctgat
    31981 cttctttcac cagcgccagc ggacgcccat tcatcagcac cagcaccagc ggtttaccgg
    32041 tggctttcag cgccgcaatc aagtcacgtt ggctttgcgg aatagtgata tcggtacgac
    32101 tggaagcttc gtgcgccatc ccctgagctt caccgaccac agccaccacc acatcagatt
    32161 gtttcgcggt ttgcaccgct tcatcaatca tctcttgcgg cgaacgcgga tcgactttga
    32221 ccgcttcttc atactgattc aggaaatcga taatgccttt gtcactggta acgttcgccc
    32281 ctttggcata cagcacttta ccgttttcac cgacggcgtt tttaatcccg gttagcacag
    32341 taacagattg atcggcgaca cctgctgccg accagcttcc catcacgtca cgcttgctgt
    32401 ctgccagcgg gccaaccacc gcaatggttg cagatttttt cagcggcagc gtttcgagac
    32461 ggtttttcag caacaccagg ctttcgcgcg ccacttcacg cgcttctttg cggtgcaggc
    32521 ggctttcggc attggtatcc accgggtcag actctttcgg ccccaaatgg ctgtatggat
    32581 cgttaaacaa tcccatatca tatttaacgt tcagtacatg gcgggcagca tcgtccagct
    32641 cttccatcgt cactttgccg gatttgatca acccaggcag atacttcgag taatactcgt
    32701 cgctcatgct catgttgatt ccggacttca gcgccacgcg taccgcatcc tctgggtctg
    32761 ccgccgtgcc atgtttaatc agttctttga ttgcgccgtg atcggaaacg gtgatgcctt
    32821 taaagcccca ctggtcgcgc agaacatctt tcagcagcca ggagtcggag gtggctggcg
    32881 tgccgttcag cgagttcagc gccaccatca ccgcaccgct gcctgcatcc agccccgctt
    32941 tgtacggcgg catatagtcg ttaaacaggc gctgcggact catatcgacg gtgttgtact
    33001 ctttaccgcc ttctaccgcg ccgtatgcgg caaagtgttt gacgctggtc atcaccgagt
    33061 agcgatctgc cgggctttta ccctgcatcg cttccaccat ggttttaccc attattgagg
    33121 tgagatacgt atcttcgcca aaaccttcgg aagcacgtcc ccagcgcgga tcgcgcgaga
    33181 catcgaccat cggtgcccag gtcatattca ggccatcatc tgccgcttca taagcagaga
    33241 cacgcccgac tgttttcacc gcatcgaggt taaaagacga ggccagaccg agactaatcg
    33301 ggaacaccgt gcgctgaccg tgcagcacgt cgtaagcaaa gaaaagagga attttcaggc
    33361 ggcttaattc catcacctga tcctgcatgg cgcggatatc ctgacgggtt acggtgttga
    33421 aaatcgcccc aacctgaccg tctttgatca tctcgcggat cgcctctttc gggttatccg
    33481 ggccgacgct gattaagcgc agctgaccaa ttttctcatc aactgtcatt ttcttaagca
    33541 gttcggtgac gaacgcatcc cgcgcttcgg gcgttaatgg atggttgccg aataaatcat
    33601 ccgccagtgc tggctgcagg gccagactca ccgcgattcc tactgaacat agccatttca
    33661 tatggatttt tcttcctttg ttgcccgacg tggcagcgaa aatggtgcaa aaaccgtagt
    33721 ttgccataag catgatggag agagaaaaag aatgctcagt ttattgtctg aattttcaaa
    33781 atattcactc gctgaattgt catacaaggc gctattctag tttgtgatat tttttcgcca
    33841 ccacaaggag tggaaaatgt cttccatgac aacaactgat aataaagcct ttttgaatga
    33901 actttcccgt ctggtcggtc attcacacct gctcaccgat cccgcaaaaa cggcccgcta
    33961 tcgcaagggc ttccgttctg gtcagggcga cgcgctggct gtcgttttcc ctggctcact
    34021 actggaattg tggcgggtgc tgaaagcctg cgtcaccgct gacaaaatta ttctgatgca
    34081 ggcggccaat acaggcctga ccgaaggatc gacgccaaac ggtaacgatt atgatcgcga
    34141 tatcgtgatc atcagcaccc tgcgtctcga caagctgcac gtactcggta agggcgaaca
    34201 ggtgctggcc tatccgggca ccacgctcta ttcactggaa aaagccctca aaccgctggg
    34261 acgcgaaccg cactcagtga ttggatcatc gtgtataggc gcatcggtca tcggcggtat
    34321 ttgtaacaac tccggcggct cgctggtgca acgtggcccg gcgtataccg aaatgtcgtt
    34381 attcgcgcgt ataaatgaag atggcaaact gacgctggtg aaccatctgg ggattgatct
    34441 gggcgaaacg ccggagcaga tccttagcaa gctggatgac gatcgcatca aagatgacga
    34501 tgtgcgccac gatggtcgtc acgcccacga ttatgactat gtccaccgcg ttcgtgatat
    34561 tgaagccgac acgcccgcac gttataacgc tgatccggat cggttatttg aatcttctgg
    34621 ttgcgcaggg aagctggccg tctttgcagt acgtcttgat accttcgaag cggaaaaaaa
    34681 tcagcaggtg ttttatatcg gcaccaacca gccggaagtg ctgaccgaaa tccgccgtca
    34741 tattctggct aatttcgaaa atctgccggt tgccggggaa tatatgcacc gggatatcta
    34801 cgatattgcg gaaaaatacg gcaaagacac cttcctgatg attgataagt taggcaccga
    34861 caagatgccg ttcttcttta atctcaaggg acgcaccgat gcgatgctgg agaaagtgaa
    34921 attcttccgt ccgcatttta ccgaccgtgc aatgcaaaaa ttcggtcacc tgttccccag
    34981 ccatttaccg ccgcgcatga aaaactggcg cgataaatac gagcatcatc tgctgttaaa
    35041 aatggcgggc gatggcgtcg gtgaagccaa atcgtggctg gtggattatt tcaaacaggc
    35101 cgagggcgat ttctttgtct gtacgccgga ggaaggcagc aaagcgtttt tacaccgttt
    35161 cgccgctgcg ggcgcagcaa ttcgttatca ggctgtgcat tccgatgaag tcgaagacat
    35221 tctggcgctg gatatcgctc tgcggcgtaa cgacaccgaa tggtatgagc atttaccgcc
    35281 ggagatcgac agccagctgg tgcacaagct ctattacggc cattttatgt gctatgtctt
    35341 ccatcaggat tacatcgtga aaaaaggcgt ggatgtgcat gcgttgaaag aacagatgct
    35401 ggaactgctt cagcagcgtg gcgcgcaata ccctgccgaa cataacgtcg gtcatttgta
    35461 taaagcaccg gagacgttgc agaagtttta tcgcgagaac gatccgacca acagtatgaa
    35521 tccggggatc ggtaaaacca gtaagcggaa aaactggcag gaagtggagt aataattacg
    35581 gatggcagag tatcgccatc cgtcattcat ttaatcgttc tgtgccgtct gccccgccgc
    35641 cgccatttgg gcggcttttt gttttttata gctcaacgct gctgccggaa caggcatcac
    35701 tttaccggtt tcaatccagg tacgcaggcg gctggcatcg gcaaaatggg tatatttgcc
    35761 aaacgcgtcc atcactacca gcgccaccgg tttattattg ataaccgtgc gcatcaccag
    35821 acaatggccc gccgcattgg taaaaccggt tttggttaac tgaatattcc agttatcacg
    35881 atacaccaga tggttagtat tgcggaacgg cagcgtatac gtcgggttag agaaggttgc
    35941 catatcttcc cgggtagtac ttaactgccc gatcaacgga tattgtttgc tggcaatgag
    36001 tagtttggtt aagtcacggg cggttgaaac gttatgcacc gacaatccgg ttggttcaac
    36061 aaagcgcgtg ttgttcattc cgagcgattt cgctttcgca ttcatcgcct taataaaggc
    36121 tttgtaacca ccgggataat ggtgcgcaag gcttgccgcc gcgcggtttt ctgaagacat
    36181 cagcgccagc aacagcatat ctttacggct gatttcgctg ttcagtcgta cgcgcgaata
    36241 gacccctttc atctccggcg tctggctgat atccactttt agtttttcat ccagcggcag
    36301 tcgtgcatcc agcacaacca tcgcggtcat taatttgctg atagacgcaa tcggacgcac
    36361 cagatccggg tggttcgaat agatcacttt gttggtattc agatcaacaa tcatcgcgct
    36421 accggaggca atttccggtt gtgacgcggt ggtagcggct gccgttttcg caacggcctg
    36481 cggtgcaaaa ggcacagcca gcatcagggc caggctaaat aaagaaactc gaaatttcgg
    36541 catgatgagc attcagatag tggttcacgc gcacgggttg cgcaccgccg gagtaaggat
    36601 ttactgaggc tagcgacgcc atcataacga gcaaaaagtg cgatcgtcaa aggagaatcg
    36661 tgaggaaatg ctgcattgct gacatttacg ccagcaatgc agcgcaaaag aactttctag
    36721 aacaaacgat aaccgtagcc ccatagtata acggtaaggg cgagcagcac ttccagcacc
    36781 agcacgccta tcgccagtgt cgaactggag aagctaagtc cttcctcttt gttgatattc
    36841 aggaagctcg gaataccgag atacagcagg tagccggtgt aaaacagcgc caccgtgccg
    36901 accagcgcac acaaccagac cagcgggtaa agcgccacca gaccgcttaa aaacagcgga
    36961 gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagtg acggacgctg cggataatta
    37021 cgcgccatcc accagatgac ccgccccatc accgcaaccc cagccagcat aacgccgtaa
    37081 aacaagacag ccagcgccag tccggtaaac caggataact tcaggatagt gccatcgcca
    37141 aaattccagc caatctgtgt agtgccaatg aaggcgcaaa tcaccggaat cgccgccatc
    37201 agcaaaacgt ggtgggtgta gtgatgagaa atcgtttcgt tttcgcgatt aatcacctgc
    37261 atttcacgat cgggatggga aaacagtccc cagacatggc tcataccgcc cccttgttgt
    37321 gagttcatga acctgacagt tcaagtataa gtcagcttgt gattattttt tgttcactca
    37381 tgtaatttgc accgtctgat gtacgtcctt tttcagcgtg aatgattcac agggtgtatg
    37441 cttacaggca agcaaaggga gacagactgt cctatggatc tcaatacact catctcacaa
    37501 tatggttatg ccgcgctggt gatcggtagc ctggcggaag gtgaaaccgt gactttgctg
    37561 ggaggcgttg cggcacatca ggggctatta aagttcccgc tggtggtact ttctgtggcg
    37621 cttggcggca tgattggcga ccaggtgctt tatctgtgcg ggcggcggtt tggcggcaag
    37681 ctattacgcc gtttctcgaa acatcaggat aaaattgagc gggcgcagaa actgatccaa
    37741 cgccatccgt atctgtttgt cattggtacg cgttttatgt atggctttcg ggtcattggc
    37801 ccgacgctga ttggtgccag ccagctgccg ccgaaaatct ttctgccact gaatatcctc
    37861 ggcgcatttg cctgggcgtt gatttttacc actatcggtt acgctggtgg tcaggtgatt
    37921 gcgccgtggt tgcataatct cgaccagcat ttgaagcact gggtctggtt gattctggtc
    37981 gtggttctgg tggtaggcgt gcgctggtgg ctgaaacgac gcgggaagaa aaagccggat
    38041 aatcaggcgt aaaaccattg cctaggataa ggcgttcgcg ccgcatccga catccggtgc
    38101 accatgcctg atgcgacgct tgtgcgtctt atcatgccta cccaaatatt acccattgaa
    38161 ataaccacgc acctcactgc gctattctgg gttgaactgc ggattcgcca gcataaagcc
    38221 gccatccact atcaacgact gcccagtggt gtaatttgcg ccctccgaac aaagccacac
    38281 caccaggctg gcaatctcat gcgttgcgcc aaaacgccgc aagggaatcg aaggctccgc
    38341 gccgggcttc acgtcgccat catccatgcc gttcattggc gtggcgatcg ccccaggtgc
    38401 aaccgcattc accaaaatct tatgcctgac cagctccagc gccatcgctt tggttaaccc
    38461 gccgagcgca tgtttagcgg ctgtgtaggc gctggcatcc ggcaacggcg tatgttcatg
    38521 caccgacgta atgttgatga tgcgaccacc ctgcccttgt ttcaccatct ggcgggcagc
    38581 aatttgcgag cataaaaatg caccatcgac atcaacggta aaaatcttgc gccactcatc
    38641 gaaagccata tcaagaaacg gcgctttggt cattgcgccc gcattattca ccagcacatc
    38701 aatacgcccc agccgttgaa tgagtttctc cagcgccagt gccccttctg gtagattgcc
    38761 gagatccagc tgcacgatct ccgcacgtac gccgtggcta actacctcac gcgcggtatc
    38821 ttttgcccct tcttcatctg agtgccaggt aatgccaata tcaaacccct gctgcgccag
    38881 taataacgcg cactctttgc cgatccccga atcggaggcg gtaataatcg cgacctgtgc
    38941 catcgagttc tccacttaac gctgaataaa cgttaagtat agaaggcgca tatcatcagc
    39001 gtttgtaacc cccgcccaac gcaccagtga gttgaatgga ggcatccagc cactgccctt
    39061 gcaataacag gccattggcc cgctcacgca gcgcggggat tctggcttcg ctgacgcggg
    39121 aacccgcaat gatgcccgcg ttaaagcgcg cctgcgcaag acctactaca cgcaaggcat
    39181 cgcgctcaat ttgcgcctga tgctggtttt tctccgccag tgtctgaacc tgactggctg
    39241 cccgcgccac gtcattcacc gcttcaacca ccgctttgtt gtagctggcg atagacaagt
    39301 tgctttcggc ttttgcgata tcgaggttgg cgttaagacg accactatcg aaaatgggta
    39361 gcgtcaggcc tgccgtaacg cccatttgct gcgcggaatg acggaacaga tcgcttaagt
    39421 gcaacgcatc ctgttgcagg aaggccatca ggttgatgtc aggataaaat gccgcttttg
    39481 ccgcatcaat agtgcttaac gatgactcaa cgtaccagtg cgccgcctgc aaatctgccc
    39541 gccgggccag taaggagtac cccagttcat caggaagctg acttgccact ttcggcaacg
    39601 cgaccggatt aagcttcaat gcctttgtct ggtgatttgt aagtgcgctt aaccgtgcct
    39661 caataatttt cattttcccc gcgacatcgt tgagctgctg ccgggttttg ctggcattaa
    39721 tatcggtttc cacaccttca actgaagaag taatcccgtt ctgatatagc tggcgatcgg
    39781 tggcgataat ggtgttctgc tctttttcga tttgctgtaa gacagtgttt aacgccgcct
    39841 gggtttgcca ctcccagtac aggcgggcta cgctgccagc cagcaattgg cgggtttgct
    39901 cgcgttccgc cgctcgtgct ttaaccgtac ccaggcgagc agttacctct gcccgattct
    39961 ttccccagat atcgagatgc cagcccgccg ttaagccaaa agtaccgttg gtgtaccacg
    40021 gaccggtcgt acctgcggca ggatcgttca gagcaaacgg ccccattaac ccttctgcag
    40081 acattttttg ccgctccata tccgcagaaa agtcgatctg cggaccatcc tgagtagcaa
    40141 ctgccttcgc ctgggcttca gccagttgaa tgcgctgttc agccacctgc atatccggtg
    40201 cgttctgtag tgcattgtta attaaggaag tgagttgatt atcgtgatac tccagccacc
    40261 attggctgtc tggccaacca tttttcagcg ccgtgggtaa tgcggtgtca acttgtgcag
    40321 cgggcgtttg ctggcttaac gcctggcggg tttcatgcat aggtgcacac ccggccagca
    40381 tcagtaacag cggaaaacag gcgatggcgg gataaaagga atcacgattc atgggggaat
    40441 aatcaggtaa gaaaaggtgc gcggagatta ccgtgtgttg cgatatattt tttagtttcg
    40501 cgtggcaata catcagtagc aataaaacga catatccaga aaaatataca ctaagtgaat
    40561 gatatcttcc gatttatctt aatcgtttat ggatagcggc aaagggcttc gttttttcct
    40621 atacttattc agcactcaca aataaaggaa cgccaatgaa aattatactc tgggctgtat
    40681 tgattatttt cctgattggg ctactggtgg tgactggcgt atttaagatg atattttaaa
    40741 aatcagatat ggcatataag ttgtcaggtc aggaactaga tattttgtgt acttgatttg
    40801 caaacagctc cggtgttggt ggcgtcaaaa tcgctgaagc gttccccgaa ggccggggca
    40861 gcccacatgg atgtgggctg agggcgcgtc ttacaaggac gttacctcgc gcccgacccg
    40921 gtagccgaag gggataagct gaaggtaccg cgcagcggcg attttgttcg ccagagcccg
    40981 ggggtgcagg gggaggaggc gattgtcctc cccctgcgcg ctccttgcgc cagtggcaat
    41041 atgtggctta gctcatgaaa ggagcgcaac aagatgatga agctgaataa gcgattcatt
    41101 gtctcagata agctgcgatt ccactattat cgcagcttat cgatatcaat ggcctgaata
    41161 gcccttgcaa tatcagggga attattcaac acccgaacat gctgaaataa ttccgttgct
    41221 tcatcgtatt ctttacgcaa ataactcaac cactgtttaa tccgcgcaac gtgatataac
    41281 ccggtatcgc cctgcttttc cagacgggta tatttttgca gcaaagcaac cacctccggc
    41341 cacggcattc gcggttcgtt atatttcacc acacggctca ggttgggaat attgagcgcc
    41401 ccgcgaccaa tcatcactgc gtcgcagccg ctgatcgcca tgcattgttg cgcactctgc
    41461 cagtcccaga tttcaccgtt ggcaatcacc ggaatattaa gccgctggcg aatctcgcca
    41521 atcgcctgcc agtcaatatg ttccgcgcgg taaccctgct cttttgtccg cccatgtact
    41581 accagctccg tagcaccagc ctgttgaacc gcatcggcga tttcaaattt cttctcaccg
    41641 ctgtcccagc ccagacgcac ttttacgctg acaggcaaat gcgccggtac agcttcgcgc
    41701 atcgcttttg caccctggta gatgagttca ggatctttca gtaacgttgc cccaccaccg
    41761 ctaccgttaa ccgttttcga cgggcagccg cagttaagat ccacgcccca ggaacctaac
    41821 tccaccgcac gggcggcgtt ctctgccagc cactgtggga actgccctaa caactgcacg
    41881 cgaaccagcg taccggatgg tgtccggctg gcattttgta gctcagggca aatgcgatga
    41941 aagactttta ctggcagcag ttgatccacc acgcggacaa actcggtgat gcacagatcg
    42001 tagtcgttaa cttcggtcag taattcccgc accagagagt caagtacacc ctccatcgga
    42061 gccagtaaca cacgcatatc atcacccgca aaaaaatgag gcgctatgtt agcgcctccg
    42121 gtcaacggat taaaggtctg caaagaggga ttgctgcaaa ctgcaatccc tctctatgct
    42181 tgatgtatgc ctgagttcgc tgcgcattgc gtaataccgg ggagcgaaaa aataagcgct
    42241 gatttcttaa tgtgatcggt agcacgtttt acgaattaat tgtatgatga atccatctca
    42301 tctggggtgt tgattatgag taagacactg aacattatct ggcaatattt acgtgctttt
    42361 gtcctgattt atgcctgcct gtatgcaggc attttcattg cttccctgct accggtaacc
    42421 attccgggca gcatcatcgg gatgctgatc ctgtttgtcc tgctggcctt gcaaattctt
    42481 ccggcgaaat gggtcaatcc ggggtgctac gtactgattc gctatatggc gctattgttt
    42541 gtgccgattg gtgtaggcgt catgcaatat tttgatttgc tccgcgcaca gtttggtccg
    42601 gtagtggttt cctgtgcagt cagtacgctg gtggtttttc tggtggtgag ctggagttca
    42661 caactggtgc atggtgaacg taaagtcgta ggtcagaaag gatcagaaga atgatggcga
    42721 atatctggtg gtcattaccg ctgacgttga ttgtcttttt tgccgcccgc aaactggcgg
    42781 cacggtataa gtttcccctg cttaacccgt tgctggtagc aatggtggtg atcattcctt
    42841 ttttaatgct gaccggcatc tcttacgaca gctactttaa aggtagcgaa gtgctaaacg
    42901 atctgctgca accggcggtc gtcgcactgg cctatccttt atatgagcag ctacaccaga
    42961 tccgcgcgcg ctggaaatcg atcatcacca tctgttttat cggcagcgtg gttgcgatgg
    43021 tgacggggac ttctgtggca ttgttgatgg gcgcttcacc ggaaatcgcc gcgtcaatcc
    43081 tgccgaaatc agtcaccacg cctattgcaa tggcagttgg cggcagtatt ggcggtattc
    43141 cggcaatcag cgcagtttgc gtgattttcg tcggcatcct cggcgctgtg tttggtcata
    43201 cattgcttaa tgcgatgcgt attcgtacca aagctgcgcg cggtctggca atggggacag
    43261 cctcgcacgc cctcggtacg gcgcgctgcg ccgagctgga ttatcaggaa ggtgcattta
    43321 gttcgctggc gctggtgtta tgcgggatta ttacttcgct gatcgcaccg ttccttttcc
    43381 cgattattct ggcagtaatg ggctaaaatt tgcgatgcgt cgcgcatttt tgatgtaagt
    43441 ttcacgcgtt gcataattaa tgagattcag atcacatata aagccatagc gggttcgtaa
    43501 actgttatcc cattacatga ttatgaggca acgccatgca tccacgtttt caaaccgctt
    43561 ttgcccaact tgcggataac ttgcaatctg cactggaacc tattctggca gacaagtact
    43621 tccccgcttt gttgaccggg gagcaagtct catcgctgaa gagcgcaacg gggctggacg
    43681 aagacgcgct ggcattcgca ctacttccgc tggcggcggc ctgtgcgcgt acgccattgt
    43741 cgaattttaa tgttggcgca attgcgcgcg gtgtgagcgg aacctggtat ttcggtgcca
    43801 atatggaatt tattggtgcg acaatgcagc aaaccgttca tgccgaacaa agcgcgatca
    43861 gccacgcctg gctgagtggt gaaaaagcgc ttgcagccat caccgttaac tacacgcctt
    43921 gtggtcactg ccgtcagttt atgaatgaac tgaacagcgg tctggatctg cgtattcatc
    43981 ttccgggacg cgaggcacac gcgctgcgtg actatctacc ggatgccttt gggccgaaag
    44041 atctggagat taaaacgctg ctgatggacg agcaggatca cggctatgcg ctgactggtg
    44101 atgcgctttc tcaggcggcg attgcggcgg caaaccgttc gcacatgcct tacagtaagt
    44161 cgccaagcgg tgtcgcgctg gaatgtaaag acggtcgtat tttcagcggc agctacgctg
    44221 aaaacgccgc attcaacccg actctgccgc cgttgcaggg ggcgttaatt ctgttgaatc
    44281 tcaagggtta tgattacccg gatatccagc gcgcggttct ggcagaaaaa gccgatgcgc
    44341 cgttgattca gtgggatgcc acctccgcaa cgctgaaagc tctcggctgt cacaatatcg
    44401 accgcgtgct tctcgcttaa gcctggtgcc ggatgcggcg tgaacgcctt gtccggcttg
    44461 ccagcccttt cctggtgtcg aaaatcccgg caaacagttt gccgtttctt gcgcaaaacc
    44521 agcgggtaaa gtagcctgat ggaaattttc cttagatcga gtctcctgca tgttaaagcg
    44581 cgtgttcctc agcctgttag tcctgatcgg cttgctgctg ttgactgtgc tcggcctcga
    44641 tcgctggatg agctggaaaa ccgcgcctta tatctacgac gaattgcagg atctccccta
    44701 ccgccaggtc ggtgtggtgc tcggcacagc aaaatattat cggactggcg taattaatca
    44761 gtattatcgc taccgcattc aaggagcgat taatgcctat aacagcggta aggtaaatta
    44821 tctattactg agcggcgata acgcattgca aagttataat gagccgatga ccatgcgcaa
    44881 agatttaatc gcagccggtg tcgacccatc agatattgtt ctcgattacg caggctttcg
    44941 tacgctggat tccatcgtac gtacacgcaa agttttcgat actaatgatt tcattattat
    45001 cacccaacgt ttccactgtg agcgggcatt atttattgcg ctgcatatgg ggattcaggc
    45061 tcagtgttat gccgtaccgt caccgaaaga tatgctgtca gtacgtattc gcgaatttgc
    45121 cgcccgtttc ggtgcgctgg ctgaccttta tatttttaaa cgtgaaccgc gttttttagg
    45181 gccgctggtc cctattccgg caatgcatca agtgccggaa gatgcgcagg ggtatcccgc
    45241 tgtcacaccc gaacagttac ttgaattaca aaagaaacaa ggaaagtagt tatggatgta
    45301 cagcagtttt ttgtcgttgc cgtttttttc cttatcccga tattttgttt ccgcgaagca
    45361 tggaaaggct ggcgcgcagg cgcgattgat aaacgggtta aaaatgcacc ggaaccggtg
    45421 tatgtctggc gagcgaaaaa tcccggactc ttcttcgctt atatggtggc atatatcggc
    45481 ttcggaattt tatctatcgg catgattgtt tatcttattt tctatcgtta atattttctc
    45541 ttttctgtta attattgaga ataattatta cttcactcaa taagctgcgg atataacatt
    45601 ctgaaccgca gctatttgtg aatcctttca cagtttaaat ttccccacgc gcatagcctt
    45661 aatatcagca cgcaatattt atttagcgtt cgccttaatt actcatagca ttaaggaaga
    45721 tcacatgccg caacaaaatt atctggatga actcactccg gcttttacgc ctttactggc
    45781 gattaaagaa gcctctcgct gtttattatg tcatgacgct ccctgtagtc aggcttgccc
    45841 ggcacagacc gatccgggga aatttattcg ctcaatctac tttcgtaatt ttaaaggcgc
    45901 tgccgagaca attcgcgaaa ataatgccct cggtgccgtt tgtgccagag tgtgcccgac
    45961 ggagaaatta tgccaaagcg gttgtacacg tgccggtgtt gatgctccca ttgatatcgg
    46021 tcgcttacag cgttttgtta ctgattttga acaacaaact gggatggaaa tttatcagcc
    46081 cggtactaaa acgctcggca aagtcgccat tattggcgca ggtcctgccg gattgcaggc
    46141 cagtgtgacg ctgacaaacc agggttatga cgtcacgatc tatgagaaag aagcacaacc
    46201 cggtggctgg ttgcgtaacg gcattccgca attccggtta ccgcaatcgg tgctggatgc
    46261 agaaatcgcc cgtatagaaa aaatgggcgt gaccattaag tgcaataacg aaatcggtaa
    46321 aacactcacc ctggagcagc tgaaagcgga aaaccgcgcg gtactggtca ccgtagggtt
    46381 atcgagcggt tccgggttat cgctgtttga gcatagcgac gttgagattg ccgttgactt
    46441 cctgcaacgt gcacgacagg cgcaaggcga tatcagcatt caacaaagcg cattaattat
    46501 cggcggcggt gatgtcgcga tggacgtagc cagcacgctg aaagttctcg gctgtcaggc
    46561 ggtaacctgc gtagcgcgtg aagagttaga tgagtttccg gcaagcgaaa aagagtttac
    46621 cagcgccaga gagctgggcg tttcgatcat cgatggattt acaccagtag ccgtggaagg
    46681 caataaagtc acctttaagc atgttcgttt accgggtgaa ctaacgatag cggcggacaa
    46741 aattattctc gctgtaggtc aacatgccga acttgctgct ttttccgcac tggaaccgca
    46801 acgtaacacc atcgaaacgc aacattacca gacccgcgac ccgcaagtct ttgctgctgg
    46861 cgatattgtt gagggtgaca aaaccgtggt ctatgcagtg aaaaccggga aagaagccgc
    46921 cggggcgatt catcactatt tagagggagc ttgctcatgt taacgaaaga tctttcgatt
    46981 actttttgcg gcgtgaagtt tcccaacccg ttctgccttt cttcttcgcc ggtaggcaac
    47041 tgctatgaga tgtgcgccaa agcctacgac acaggttggg gcggcgtggt gtttaaaacg
    47101 atcggttttt ttatcgccaa cgaagtctcg ccgcgttttg atcatctggt gaaagaagat
    47161 accggtttta tcggcttcaa aaatatggag cagattgctg aacatccgtt ggaagagaat
    47221 ctggccgccc tgcgtcggct gaaagaagat tacccggaca aagtattgat cgcttcaatc
    47281 atgggcgaaa atgagcagca atgggaagag ctggcgcgtc tggtgcaaga agctggcgcg
    47341 gatatgatcg agtgtaactt ctcctgtccg caaatgactt ctcatgcgat gggtagcgat
    47401 gtcgggcaaa gcccggagct ggtagaaaaa tattgtcggg cagtaaaacg tggttccacg
    47461 ctgccaatgc tggcgaagat gacgccgaat attggtgata tgtgcgaagt ggcgctggcg
    47521 gcgaagcgcg gcggcgcaga tggcattgcg gcgattaaca ccgttaaatc catcaccaat
    47581 atcgatctta atcagaaaat tggtatgccg atcgttaacg gtaaatcgag tatttccgga
    47641 tattccggta aagcggtaaa accgatcgcc ctacgcttta ttcagcaaat gcgcacccat
    47701 ccagaactgc gcgatttccc gatcagcggt atcggcggca ttgaaacctg ggaagatgcg
    47761 gcggagtttt tattgctcgg ttcagcgacg ttgcaggtga ccaccggcat tatgcagtac
    47821 gggtatcgga tagtggaaga tatggcaagc gggttgtcgc attatctcgc cgatcaaggg
    47881 tttgattcgt tgcaggagat ggtaggtctg gcgaatcaca atattgtccc ggcggaagat
    47941 ttagaccgca gttatattgt ctatccccat atcaatcttg ataaatgtgt tggctgtgga
    48001 cgctgttata tttcctgtta cgacggcggt caccaggcga tggaatggag cgagaaaacc
    48061 cgcacaccgc attgtaatac cgagaaatgc gtgggttgtc tgctttgtgg tcacgtctgc
    48121 ccggtaggtt gtattgatct tggggaagtg aagtttaaga aaggcgagaa agaacacccg
    48181 gtaacgttgt aaaaccagcg acgcatccgg catttagttc ctgtcgccag atgcggcgtg
    48241 aatgccttac ccggcataca aaacaatata aatcagtatg ttgtgctcat tgtaggccgg
    48301 ataaaacgcg ccagcgttgc atccggcatt tggctcctgt cgccggatgc ggcgtaaacg
    48361 ccttatccgg cctacaaaac cacacagatc atgatgttat gtgcgtttat gaacgaatgc
    48421 ggaccacatt cacatcattt cttacgcgcg tatttcagtg aatccagcgc cacggcaaag
    48481 ataataattg cccctttgat gatgtactgc cagtacgggt ttacgccgat gtacgtcagg
    48541 ccatagttga tgacggtaaa aataatcacc ccggtcacca cgccaatcac cgtccctaca
    48601 ccgccgctga acgatacgcc gcctaccacg cacgctgcga tagcatccag ctcatacatg
    48661 aagccgaggt tgttggtggc agagccgata cgtccggctt ctaacatccc gccgaaggca
    48721 tagaacacac cagacaacgc gtagatcatc agcaggttca ggccgacgtt gacaccagat
    48781 actttcgccg cttccgggtt accgccaatg gcaaaaatgt tcttgccgaa gcgggttttg
    48841 ttccataaca cccagacaaa caccaccgca atcaacgcat agaaggtgat gtaagagaga
    48901 cggaaactcc ccagcgcaat aaagccttgg gcaaaagtgg agaaaccact gtcaaaaccg
    48961 gaaattggcg acgccccgac aaagtcgtaa tagagcgagt taatgccgta gacgataatc
    49021 atcgtaccga gcgtggtaat gaacggcgtc acgttgagat aggcgataat caagccgttg
    49081 atcaaaccga tcaccgcacc aatggcacag acaatcagaa taaccagcgc aatcggcatc
    49141 gtcgccattt ccgggaacac tttgttggcg ttatccatgg actgcaataa cgtcgctgct
    49201 actactgccg ccagccccac ctggcgacct gcggaaaggt cagtcccctg ggtgacaatt
    49261 aaccctgcca caccaagcgc gataataata cgcaccgatg actgggtgag aatattactt
    49321 aagttcaaca gacttaaaaa tgttgggtcc tggaaaataa taatcgccag caaaactaaa
    49381 agaacgacgt aaataccgcc ctctttcagg taagtaagaa aacttttctt atttaacgca
    49441 ctcatgggaa gcccctaatc ttaaaggtgc aaagacgcaa gacgcagaat ttcgctttgc
    49501 gttgttgttt ttgtatcgac aattccggaa acgagaccat tgctcatgac cagaatacgg
    49561 tctgttatcc ctaacaactc tggcatttcg gaggagataa taataatccc cttgcctttc
    49621 ttcgccagtt cggcaattaa ctgataaatc tcaaacttag ccccaacatc aataccgcgc
    49681 gtcggttcat cgagcattaa tatttctggt tgcgttaaca gccagcgacc aataatcact
    49741 ttttgctgat taccaccgga gagcgaacca atttgcgtcc gatgccccgg tgtttttacc
    49801 cgcatcgaat caatcaccca ctgggtatcg cttttcatcc gcgagttatc cagtaaacca
    49861 actttatttt tatagttgcg gatattggaa attaacgagt taaagccaat atccagatag
    49921 gcataaattc ccgttgagcg gcgctcttca gttaccagtg caaatccgtg gtttatggct
    49981 tcgttggcat tatggttatt gatctttttg ccgtgcaacg taatggtgcc agccgatttc
    50041 tcgcgaatac caaataacgt ctcaacaata tcggtacgtt tcgctcccac cagaccggca
    50101 ataccgagga tctccccttt atgcagatca aacgagacat cgcgaatcga cggctggcgc
    50161 agcgacgtca ggttacgcac ttccagaatc acttcgcccg gcttgttttc tttgtcaggg
    50221 aaacgctggt taagagaacg cccaaccatc atggcgatga ttttgtccat cgtcagtcct
    50281 gccagcggtt cggtagcgat ccactgaccg tcgcgcaata cggtaacttc atcacataac
    50341 tggaagattt cttccatttt atgcgagata taaacaatac cgcagccgcg ctcttttaat
    50401 ttacgaataa tagtgaacag atgattgacc tctttttcgg ttaacgaaga agtcggttca
    50461 tccataatca caattttcgc gttataggaa aacgctttgg cgatttcgat catctgcatt
    50521 tgcgaaacag ataatgtacc gacgcgcgca cgcggatcga tatcaatatc cagttcatca
    50581 aaaatcgctt tggtttcgcg gtacattttg tcctgatcga caaacatgcc tttggtggga
    50641 tatcgcccca gccacatgtt gtccatcacc gaacgttgta ataccaggtt taactcctga
    50701 tgtaccatcg aaataccatt ttccagtgct tctttggcgg aatggaaatc gatctcttta
    50761 ccctggaata aaatggtgcc ggagtctttt tgataaatac caaacaggca ttttaataat
    50821 gtcgattttc ctgcaccgtt ttcccccatt aatgcatgga tagaatgtgg ccggactttt
    50881 aagttaacgt tatcaagtgc cttaacacca ggaaaagact tgttgatacc gctcatttcc
    50941 aacaagtatt cacctgatga cggagtcgtt gagctgacca tataatttta ccttgttggt
    51001 catacaataa gggcgcagta aacgactgcg cccaatcagt cttatttctt gctgaattca
    51061 gccaggttgt ctttatctac gccaacataa ggtacgcgga ccactttgtt gtcgattttc
    51121 cagttggtgc catcagccgc acctttgcca tcggccaggt ttttcgccag atcaaaggtc
    51181 gctttcgcct ggttgttagc atcgttcagt acggtgcccg ccagtgcacc ggatttcacc
    51241 agcgccagcg cttctggcag cgcatcgacg ccaaacaccg gaatgctgga cttgttgtgt
    51301 gctttcagtg cttctaccgc gcccattgcc atcgcgtcgt tgttggcgat aaccacctcg
    51361 attttgttgg cgttcgggcc agacagccag gcgtccatct tatctttcgc ctgagcggta
    51421 tcccacatag cggtatctaa ctgtaactgt tcagttttaa tgcctttgtc gttcaattct
    51481 ttaatcacgt aagtggtacg tgcttctgca tccggatgcc ccggctcacc tttcagcagt
    51541 acgaattgaa tctgaccgtc tttgttcaga tcccaaccct gattcgccgc ccagtgttta
    51601 gcaatcaaat cgccctgaat aatgccggac tctttggagt cagtgccaac gtagtaggct
    51661 ttgtcgtagc tatccagcgc cttacgagac ggttctttgt tgaagaaaac caccggcacg
    51721 ttttgcccac gcgctttctc aatcaccgta cccgcagctg ccgggtcaac caggttgatt
    51781 gccagtgcct tcaccccttt cgccagcaat acgtcgatct gatcgttctg cttggactgg
    51841 tcattctgag aatcattcat cagcagctga acatctggcg cggctttcgc atcttgctca
    51901 atagccttgc gcactacaga cataaagtta tcgtcgtact tatagattgt tacaccaatg
    51961 cgagtatcag cagcgtgtgc agcggcaccg aataacatgc tggccatcac agcagacagg
    52021 gttaacacct tcttattcat ggtatctccg gtttttctta tgcagggtag tgcttgagat
    52081 aaatgctcgg cggggcagta gagttaatga agtgttactg aacgccgaag ctcacttttt
    52141 aaaattcgtt cttccatgcc cggtaacgct ccagaaaacg gctttaattg ttgttatgac
    52201 gctattacct cggtgaaagt gattattcac tgttacatat gggttacaac gttaaaacgg
    52261 tgcaatcata gccatcacat tgttaagata ctgtgaaatc actcacagat tgaaagcggt
    52321 tacatcgcct gatttgttga gttagtgatc gccgccgcat tctggcgtgt tgcgacagaa
    52381 tggcgacgca ctaacgtcgg cataaaacag tggctggcac gaggatcaat attgtctgct
    52441 gccccctgca aggccagttc ggtagctaat ttagccattg aagcaatggg ataacgcacg
    52501 gtcgttaatt gcgggtcggt gtaacgggcg atgggaatat catcgaaacc gatgattgag
    52561 agatgtagcg gaatcgcaat gccattatct tttaatgctg tcagtgcgcc agcagccata
    52621 ttgtcgttat aagcaaatac agcggttagt tgtagattgc gccccagcag ttcaaccatc
    52681 gccgcctcac cgcccggcat gtccggcgta ccagtgccaa tccagctttc cggcggaata
    52741 atatcctgct ctttcaacgc actcatccag cctgctttac gcatggcgtc atcttcaatg
    52801 ccgtggctgg aagaaagata accaatacgt tgatgaccgt tattcagcaa catgcgcgtc
    52861 gccattcggg caccgctgag attatccagg caaacgcaac gatgggcgta ccccggcaca
    52921 acgcggttga ttaacaccat accaggaatg ttatccataa attgtgccag ttcatcgtca
    52981 ctcaatgctt ttgagtgaac aatcaacgca ttacaacgct ggcgaattaa cacctcaatg
    53041 gcgtgacgct ctttttccgc ttcatgatag ctattgccga ttagcacgta tttctgatgc
    53101 tgctgggcga ccagatccac cgcttttacc agcgcgccga aaaaagcatc agaaacgtcc
    53161 atcaccacca cgccaatggt gtcgctaacc tgagttgcca gcgcctgggc attggcgttt
    53221 ggccgataat ccagctcact cacagctttc attactgctt cacgcgtgtc ggcactgacc
    53281 agcgtgctgt tattgagcac ccgggaaacc gttgccacag agacgccagc ctgacgcgct
    53341 acatcacgaa tggtgatcat attcactacc tgttatgaga ttgcagtaaa tgactgcttg
    53401 ctggcggcta ttttgtcagc actgaataca ggacttcgtg aatcgagtca cagcaatgga
    53461 aacggttaca gccgttttgt taacgaatgt gatccagatc gttatcttga tgttttagat
    53521 attgtcggtc cggcagcacg caaagttaac tgacgccaga gccattccac cggcccctgg
    53581 cggaagtaac gcagccagat aacagagaag aagatattcg ccagccatac cggaataaca
    53641 aacgccagca gctccagacg gtcaaactgc ataaacaaac tgaggtggta aaaaagcgta
    53701 gtacaaatca gcgtttgcaa tagatagttg gtcagtgcca tccgtccgac acaggcgatt
    53761 gccagcacca gcttaaagcg gcttaattgc ggccagaagc cataaaacag cgacgcatag
    53821 ccaatcgcct gaaacggcgc actcagttcc cgcggcattt gaagtaagaa ggcacaccag
    53881 cgatacgccc agtccagctg ccattgcagg gcgatggcag gaaggttaat gatcacccca
    53941 atcgccacca gcacaaaacc agtacgacga tagtgacgta agctgaactg ccctttcagc
    54001 cagccgctgc gcatcagcgc cgcaccaatg agcatcatcc ctgccagttg ccagccatac
    54061 tgtgcgccca gtgccagtaa actgttgcca acaccatcgg cacgattatt gatcgcttca
    54121 acaccaccgt gaagcttcca gtatttttca tacaaaatag ccgatgcatc cggcgtccag
    54181 gcgcggctgg tctggctgtc ggaaatcagc cccaataaca gcaaaacgcc aagccccacc
    54241 agataaagca tgacgcctgt attaaacagg ctttttaccg atggcgcatc gcgcaccagc
    54301 cgccagcaga ttaagcccac cagtccgtaa gccagcagaa tatcgccgtc ccagaacaat
    54361 aaaccgtgaa taaagcccag caagactaac agcgttaacc gcgactggat ccagcgtctg
    54421 ccacggggca gcaacatttg caggcccgca ccaaacagca gcgcaaaaag cgtgaggaat
    54481 ttcacctggc cgatgagatc gagaaatgcc caggtccatg catcctgcgg cgtaatagcg
    54541 ccgtaccagg cgggattgag ataagccgcc tttggtagcc caaaggcgct gatgtttaat
    54601 agcaggatcc ccagaatggc gacgccgcga acaaaatcga gcgtgacgtt gcgctccatg
    54661 gctcctgcct tttaatcagt tgtgatgacg cacagcgcgc agaaactcgt ggcgcgtatt
    54721 ctgactggat ttgaacaatc caccaagaga ggtcgttgtt gtggcactgg ttgcgtcgcg
    54781 gatgccacgc gctttcacgc agtaatgcac cgcgtcgata gagacagcca cgttattggt
    54841 gcccagcagc gtttgtagcg caatgagaat ttgctgcgtc agacgttcct gcacctgcgg
    54901 acgctgggca aagaactgca cgatgcggtt aatttttgac agaccgatca ccgaatcttt
    54961 cgggatataa gccaccgtcg ctttgccatc tatggtaaca aaatggtgtt cacaggtgct
    55021 ggtcagggtg atatcgcgca cggtgaccat ttcatcgacc ttcattttgt tttcaatgag
    55081 ggtgattttc gggaaattgg cgtaatccag accggagaaa atttcatcga catacatttt
    55141 agcgatgcga tgcggcgttt ccatcaaact gtcatcagcc aggtcgagat tcagcagctg
    55201 catgatttcg gtcatatgac cagcaataag gcttttgcgc gtttcgttat ccatttcatg
    55261 cacgggcggg cgcagcggtg tttccagtcc tcgcgcaact aacgcttcat gaaccagggc
    55321 cgcttcttta ctgagtgatg gcatttatga tttctcctgc aggtgtgacg cctccgccct
    55381 gcgtgggggc aaagttatta agctgattta cagcctgatt attgtgcgtg aggcggcgca
    55441 cataatccag tattcacagc gataattatt gtaattgccg ctgcctttca tcagcagatg
    55501 ttaaaacatc gttatgcaaa tacggaagtg aaagttactc acagcacatt gaataaacgg
    55561 tatgatgaag aaattgcaaa caacacaaca aggagccacg catggaaatg ctcgaagagc
    55621 accgctgttt tgaaggctgg cagcaacgct ggcgacacga ctccagtacc ttaaactgcc
    55681 cgatgacgtt cagtatcttt ctcccaccac ctcgtgatca caccccgcca ccagtgctgt
    55741 actggctttc cggattaacc tgcaatgacg agaacttcac caccaaggcg ggtgcccagc
    55801 gcgtggcggc ggaactgggg attgtactgg taatgccaga caccagcccg cgcggcgaac
    55861 aggttgccaa cgacgatggc tacgatttag gccagggcgc ggggttttat cttaacgcca
    55921 cgcaaccacc gtgggcgacg cattaccaga tgtatgatta tctgcgcgat gaattaccgg
    55981 cactgattca gtcgcaattt aatgtcagcg accgctgcgc tattagcggt cattccatgg
    56041 gtggtcacgg cgcgctgatt atggcgctga aaaatccggg taaatacacc agcgtttccg
    56101 cctttgcgcc aattgtgaat ccgtgcagcg tcccgtgggg aatcaaagcg tttagcactt
    56161 atttaggtga ggacaaaaat gcatggctgg aatgggacag ttgcgcgttg atgtatgcca
    56221 gtaacgcgca ggatgcaatc ccgacgctta ttgatcaggg tgataatgat cagtttcttg
    56281 ctgaccaact gcaacctgca gtactggcag aagccgcgcg ccagaaagcg tggccgatga
    56341 cgctgcgtat tcagccggga tatgatcaca gttactactt catcgcctct tttatagagg
    56401 atcacctgcg cttccatgcg cagtatttac tgaagtgaaa gtccgcccgg ttcgccgggc
    56461 atcttctcat cagaagcgat aatccactgc cataaagtaa cgacgtccgt cttcgttata
    56521 gctgtagtcg tcacgactga gatccttgtc gccaaggttc agcacgcctg cacgcagttt
    56581 gacgtcttta gtcacctgcc aggccgcgcc ggtattccag atggtataac cgcccggtgt
    56641 tttagccgtc gcgctgtcgg cgcgtttctg cccggtatag tgaccagaaa catagaatga
    56701 ccagtcttcc agcgccagcg gtttccagtc cagcgtaccg ttagcagtat ggaacggcag
    56761 atcggatagc ggtttgtttt cgccgttgct gacatcacga ccatcgttgt aggtgtagtt
    56821 gatcgacagt ttccattcat cgttgaacgg aattttcagt tcggtttcca cgccctgaat
    56881 acgtgctttg ttaacgttgt agtagctaaa taccggtatg cgccgtccgt tagcgcccgt
    56941 ctcaaaaccg acaaagtttt ggtagcccgg tgcagcgttg acgtcagacg tacggctaat
    57001 gctgatacga tctttcacat cgttacggaa aacggtaacg ctggattcaa ccccttccag
    57061 ccagccttct tcgcccatgt agtaaagccc cagttcccaa ctttcgctgg tttctggttt
    57121 cagatccggg ctacccacaa tcttacatgc gccacggcag gaattgctcg tccagtcagg
    57181 gctaagttgc aacagagaag gtgctttaaa tgccgtcgcc cagccccctt tcaccgttac
    57241 ggtgtcggtg gcgttataaa ccaggtaggc acgcggactc cagtgttcac cgtaggtttc
    57301 gtgatcgtcc atacgcacgc cggtcgtcag cgccagcggc tcgaagatcc gccattcatc
    57361 ttccacaaac agcgcgtact ggctggcaga cgttttggag ctggttcccc cggtcaggtt
    57421 caccgcatcg ctaagtttgt cgtgacgcca ttcaccgcca accgtgagaa actgattaat
    57481 cgccgtcagc ggcaacgtgt atttgccgtc gaccgtattg ctttcggaag ttatcgggct
    57541 gctgttgcca gggtttttgt tctcgacttt ctcaccgtag tatttcagtt cgctggtgcc
    57601 gtaatcccaa cgcccattat ggctgacgga gtagttctgg cgttccaggc ggtttttgtc
    57661 cagcgagtcg gaatcacgat cctgacggtc gaaaccgtat ccggcagtaa aatcgtgatt
    57721 ttgattcggt gtccaggcaa attcgacatt gccgtcgcgg ctggagaatc cttcaatacg
    57781 cggcgtttct ccggtatcgg tggtcgttga gttttgcggg tcatcctttt cacgttttgc
    57841 caggctgccg taagctttca ttcccagcac gccgtcaatt aatggtccgc tggtaaagaa
    57901 ctgaccgtta taggtatcac cgcgatcgcg atgttcctga acggtggtat cgacggtaac
    57961 ggtgcccgac catttctgac cgattttttt ggtgatgata ttcactacac cgccgagcgc
    58021 atcggaaccg tacagcgacg acatcgggcc acggaccact tcaatacgtt cgatggaatc
    58081 gaccgggatc cagttcagat cgaaatcatt gtggcggaag acggcattgc gggagttaac
    58141 gcgtttaccg tcgacaagaa tcagggtgta gctgctgtcc agaccacgaa tacttacgcc
    58201 cttacggtta tccccttcgt tcgtcagttg tacgccaggc acttctttga ggacatcctt
    58261 cagattctgt accggttttc gctgcaggtc ttcctgggta atgacgctga tactggcggg
    58321 agcatcttta aggttttgtt caacggaaga tgcagtgaca accatcgttt cgccatcatc
    58381 atcgaccgct aacacaggcc atgcacaaga aatagcggac aaacacagcc cgacccgtac
    58441 gaaagggttc aacctaaaca ttccatatct ccatgaggta actacgaaaa taaaatgggt
    58501 atcgctcaca tcttctttac gtccccttgc gtacggcagc atcgcggtgg acttatttgt
    58561 atgcaggtga tcatcccaga aagccggaac agcctctgat ttgtcagcct tttttgattg
    58621 cggcgtaacg ataatgcaaa cgataacaat tatcaatcca aatgttaaat tttatatccg
    58681 catgatttgt cagggaaatg gaattgaaaa agccctctcg gttgagaggg cttagcaagg
    58741 aagggaggaa acttatttct tatcgttctg cgggaacttc atttcgctgt agcgtacgaa
    58801 gtgagttcct ttaatcagct tatagccgaa ccaaataatc aggaacagcg ggataccaat
    58861 atacgttgcc gctacgccgc cccagtcaat ggtatctttc aggaacgctt cgtagttctg
    58921 gcccaaagtg ataatcagac acagaatgaa tgcgaagatc ggccccagtg ggaagaaacc
    58981 tgaacggtac ggcagatcgt taatgtcgtg tccctgcaat acgtacccgc gacggaaacg
    59041 atagtggcta atggcaatcc ccagccaggc gataaaaccc gtcatcccgg aggtgttcag
    59101 cagccacagg tataccgtct ggttgccaaa catggaggtc aggaagcaca aaccggcaat
    59161 caccgtcgtc gcatacagcg cattacgcgg cacgccacca cgcgacagtt tagcgaaaat
    59221 gcgcggcgct ttaccgtcac acgccagggt gtacagcata cgagtagacg catacatacc
    59281 ggagttaccc gctgacagca ccgccgtcag aataactgcg ttcatcaccg ccgccgcaga
    59341 gagcagaccc gcgtgctgga acaccagggt gaacggactg acgctgatgt ctttaacatc
    59401 gttacgcagc aggctcggat cggtgtacgg aataatcagg ctgataatca ggatcgcgaa
    59461 cacatagaac aacaggattc gccagaatac ctgacgtacc gcgcgtggaa tgtttttcgc
    59521 cggatcttcg gactcgcctg cagcaatacc gatcagctcg gttccctgga aagagaagcc
    59581 gacaatcata gctacgccga tcatcgccgc aaaaccacca gcaaacggcg cttcgccgat
    59641 tgtccagttg ctccagcccg caggctgcgc gcctttgaag ataccgataa tcatcagcac
    59701 gccaacgatg ataaagacaa taactgtcgt gactttgatc agtgagaacc agtattccgc
    59761 ttcaccaaag ccgcgaacag agatgtagtt caacaggaag ataacgccga ggaacaacgc
    59821 actccagatc cagcccggtg tatccgggaa ccaccagctc atgaccagct gtgctgcaac
    59881 caggtcaacg gcaatagtca ccgcccagtt gtaccagtag ttccagccca gcgcgaagcc
    59941 aaagccttct tcaacatagt tctgaccgta cgtggcaaac gaaccggaaa ccggcatata
    60001 tgcagccagt tcaccgagac tggtcatcag gaagtaaacc atcaggccaa tcagcatata
    60061 cgagagcaat gccccgcccg gacctgcctg agaaatcgtt gcgccagagg caacaaaaag
    60121 acctgtaccg atggaaccgc caatggcaat catcgtcagg tgacgcgcct tcaattcacg
    60181 gcgtaagccc ggcgcttctg tagttttagt ttcggaaccc atacaaaaat gctatccatc
    60241 ttaaaaatga ggcgcgattg tagcagacga ttggcaatcc ttccggcaga aatacacggt
    60301 tataagacac cttcatgatc gcccagggat tataagtaaa gcagccaatc tcttttctgg
    60361 cgaatgcatg ctgagggcgc tgcgtcacga aatcgacaca gcaccagcat gttcttgtac
    60421 agcaccttaa cgcggtacat tcgcgggatc gcaatagtcc agaaagcgcc gtagcgcgtt
    60481 ggaaaggtgt ttttgccgat gatgtatacg ccacaacgta cgcatcaggc gcggcagagg
    60541 gaccgcaact tcacttaatg tgcctgcctg caattgatct tcaatcaccc gtcgcgacag
    60601 gcaactaatt cccaacccat gacgcaccgc atgtttgatt gcctcggagt tacctaattc
    60661 catcgccatc tcaaacttcg gtaaatgtga cagcaacaga taatcgacaa tctcccgcgt
    60721 gccggaaccg cgttcacgca ggatccacgg cgcagcggcc agctgttcta aggtgaccgg
    60781 accacgagcc aacggcgaag tcggcgcggc gaaaaccacc agctcgtctt ccagccacgg
    60841 ttcagaaatg atttcagtgc tgtggcacgg tccttcaata aagccaatat caacgcggaa
    60901 atccagcacc gcttgcatca cgtcctggct attcccaacg ctaagttcaa tcggcaactg
    60961 cggataatca tggcgataac gggcgataac tgcaggtaga atgtagttac cgatggtact
    61021 actggcatag atacgaatcg cgccgttgtc ttcgcgaaac agttgttcga tttcaaccgc
    61081 ctgttcaagc agtgccaacg ctcgcggata gagcagccgc ccgtgttcat taacaaccag
    61141 tcttttcccc acgcgatcaa acagttgaac gccaagctgc ccttccaggt cggtcaaggc
    61201 tgcgctcact gctgattgcg acaacgccag catcaccgac gcctgggtgg ttgatccact
    61261 tttcaatact tctgcaaaaa cttccaactg ccggagggtg atgtgcatag tcgcttacca
    61321 cttataaaga ttaattataa atatataatc aattttattt ttaaaccagt tagtcgtaac
    61381 cttatgcccg gttaaaggag agggttatga cgaatatcac cttacagaaa caacatcgta
    61441 cactgtggca ttttattccg gggttagccc tgagtgcagt tatcaccggg gtcgccctgt
    61501 ggggtggttc cattcccgcg gttgcgggtg ccgggtttag tgccctcacc ctcgcaatct
    61561 tgttggggat ggttttaggc aacaccattt acccgcacat ctggaaaagc tgtgacggtg
    61621 gtgtgctgtt tgccaagcaa tatttattac gtctgggtat cattctttat ggcttccgtc
    61681 tgacgttctc gcaaattgcc gatgtcggta tcagtgggat catcattgac gtgttgacgc
    61741 tgtccagtac cttcctgctg gcttgcttcc tggggcagaa agtgtttggt ctggataagc
    61801 acaccagctg gttgatcggt gccggtagca gtatctgtgg tgctgccgcg gtactggcga
    61861 ctgagccggt agtgaaagca gaagccagta aagtaaccgt ggctgttgca accgttgtta
    61921 tcttcgggac cgtcgcgatt ttcctctacc cggcgatata tccgctgatg tctcaatggt
    61981 ttagtccgga aactttcggt atctacattg gttctactgt gcacgaagtg gcgcaggtgg
    62041 tggcggcagg tcatgccatc agcccggatg cagaaaacgc agcagttatt tccaaaatgc
    62101 tgcgcgtgat gatgctggct ccgttcctca tcctgctggc ggcgcgtgtt aaacagctgt
    62161 ctgggacgaa cagcggcgag aaaagcaaaa tcactattcc gtggtttgcc atcttgttca
    62221 tcgtcgttgc catctttaac tcgttccacc tgttaccgca gagcgtggtg aacatgctgg
    62281 taacgctgga taccttcctg ctggcaatgg cgatggcggc gctgggtctg accactcacg
    62341 tcagcgcgct gaaaaaagcc ggggcgaaac ctctgctgat ggcactggta ctgtttgcct
    62401 ggctgattgt tggtggtggt gcgattaact atgtgattca aagcgtcatc gcataaacca
    62461 ctacatcttg ctcctgttaa cccgctatca ttaccgtttt cctccagcgg gtttaacagg
    62521 agtcctcgca tgaaatacat tggagcgcac gttagtgctg ctggcggtct ggcaaatgcc
    62581 gcaattcgcg ccgccgaaat cgacgcaacc gcgtttgcct tgttcaccaa aaaccaacgt
    62641 cagtggcgtg ccgcaccgct cacgacgcaa accatcgatg aattcaaagc cgcctgtgaa
    62701 aaatatcact acacatcggc gcaaattctt ccccacgaca gttatctgat taacctcgga
    62761 catccggttg ctgaagctct ggaaaaatcg cgcgatgcct ttatagatga aatgcagcgt
    62821 tgcgaacagc tggggctttc tttgctcaac ttccaccctg gcagccatct gatgcagatt
    62881 tcagaagagg attgccttgc gcgtattgcc gaatccatca acattgcgct ggataaaact
    62941 caaggtgtca ccgcggtgat tgaaaacacc gccggtcagg gcagtaactt agggtttaaa
    63001 ttcgaacatc tcgcggcgat tatcgacggc gtggaagata aatcccgcgt cggcgtctgc
    63061 attgatacct gccatgcttt cgctgccggg tatgatttgc gtactcctgc cgaatgcgag
    63121 aaaacgttcg ctgattttgc ccgtattgta ggctttaagt atctgcgcgg gatgcacctc
    63181 aacgatgcga aaagcacctt tggcagccgc gttgaccgtc atcatagcct cggcgaaggc
    63241 aatatcggtc atgatgcgtt ccgctggatc atgcaggacg accgtttcga cggcattccg
    63301 ctgatccttg aaaccatcaa cccggatatc tgggcagaag agatcgcctg gctgaaagcg
    63361 caacaaactg aaaaagcggt ggcctgatcg atgaacgaca gggaaaaaca gattcttaaa
    63421 attttacggc gtaacccgct gattcaacag aatgaaatcg ccgatattct gcaaatcagc
    63481 cgctcacgtg ttgccgctca tattatggat ttgatgcgca agggtttgat taaaggcaaa
    63541 ggatatatcc ttaccgagca ggattactgt gtggttgtgg gtgcaatcaa tatggatatt
    63601 cgcggaatgg cggatatccg ttacccgcaa gcagcctctc atccaggttc tgttcactgt
    63661 tccgcaggcg gcgtcggacg taatatcgcg cataacctgg ctctgcttgg tcgggatgtg
    63721 catttaattt cagctattgg caacgatttt tacggcgaga cattactgga agaaacgcgc
    63781 cgcgcgggcg tgaacgtttc aaactgcatt cgtctgcatg gacacagcac tgcaacttat
    63841 ctcgctatcg ctaataagca ggaagaaact atcctggcaa tcaatgacac acacattctg
    63901 caacagctaa cgcctcaact gctcaatact tcacgggact tgattcgcca tgctggtgtg
    63961 gtgctggctg attgtaatct cacacctgaa gccctggagt gggtctttac cattgctgat
    64021 gaaattccga tgtttgtcga taccgtttcc gaatttaaag cgaacaaagt aaaaaactgg
    64081 tacagccgga tccacactct gaaaccaaca caaaatgaac tggaaatttt gtgggggcaa
    64141 ccgattaaag acgacaacga tcgcatcaga gctgtaaatt cgctgcatca acaaggtgtt
    64201 aaacgcattt ttgtttatct gaaagatgaa tctgtatttt gcagcgataa ggatggagaa
    64261 caatttttgc tgaccgcacc agcgcatacg acagtagaca gttttggtgc tgacgatggt
    64321 tttatggcgg gcctggtata tagctttctt gaaggataca gtttccgcga cagcgcccgt
    64381 tttgcgatag cctgcgcggc aatttcgcgt gccagcggca gcttaaacaa ccctaccctg
    64441 tctgccgata acgcgctttc attagtgcca atggtgtaac aatgttgccg gattcgacgc
    64501 ttgaccgcat cttatccggc tcataaattg cgcactacgc taaaccaata aagaatcctg
    64561 caatagtagc actcatcagg ttactgacag caaaaacgca atagccagca ataccaccat
    64621 tcccagaaca cttctcatga catccatagt tattatctct tcatgtcatt ttgggcgacg
    64681 gctctctgcc catcataaat agccgggcct gcatcttgcg atactgaccc ggcttgttat
    64741 caacacttaa tgggttttga tgtagccgcg tacgcactct tcgactaatc cccagaacca
    64801 gtctgtatca atagtgatgc cgactttggt attggcgggc ttgccaagaa cgcccaactc
    64861 gtcgcagacg gtacgcccat agcaagggcc actgttaacg tccacttcga cgtacatctc
    64921 ctgggtttta atgccatcag ggttaatcaa ataaccgatg caggtggcgt cgtgcaccgg
    64981 gccgccagcc aggccgtagt tttcgaactg cgttttgaga gtgaagttca tgatgtcgct
    65041 gaacagctct cccgcagggc cgccagccct ttccatccga gcaatcacgt ccggggtgca
    65101 aacggtctgg ttggtaagat cgaggcccat catcactaat ggaacgccgg aggtgaacac
    65161 cacgcgtgcg gcttccgggt cagcaaagat attgaactca gcagatgggg tgaagttgcc
    65221 agtaccataa gcgccgccca tcagaacaat ttcacggatt ttcggcagga tcgcgggttg
    65281 catacgcatt gccaccgcga tgtttgacaa cggaccaacc ggcaccagag tgatatcgcc
    65341 atcgcttgcc ataagggtat cgatgatata tttcaccgca tgagtgtttt ctgcctggcg
    65401 ggtcagcggc tcgaataccg ggccatccag tccggtttca ccgtgaatat tatcggcaac
    65461 aatttgttga cgcataatgg gctgcggcat ccccgcataa accggaacat taatctccag
    65521 tttctggcaa acattcaggc cattaattaa tgttttatcg agggtttgat tacccgctac
    65581 aatggtgata cctaataaat caatcgcagg atgtttcgcc gccatcatta tagcaatagc
    65641 atcatcatga cccggatcac aatccagaat aatttttctc ttttccattg tttatttcct
    65701 ctgtttccag ttgcgttatt gtttctacag caaagaaaat tcgacctggc cgatgattga
    65761 atcttaacaa cagcgtacgt atgctaaata tgagaaatct catagcggat aaacatcgtg
    65821 aaagaaatcc acaataatga tcttaagcag caattgatga gtgaatctgc gtttaaggat
    65881 tgcttttcaa cggatgtttc agccgatacg cggctgtttc attttttagc gcgtgactac
    65941 attgtgcagg aagggcaaca gccgtcctgg ctgttttacc tgacgcgagg ccgcgccagg
    66001 ctttacgtca cgcttgctaa tggtcgcgtg tcgctgatcg atttctttgc cgcgccctgt
    66061 tttattggcg agattgagtt aatcgacaaa gaccatgaac cgcgtgcagt gcaggctatt
    66121 gaagagtgtt ggtgccttgc gctccctatg aagcattacc gcccgctgtt attaaacgac
    66181 acgctatttt tacgaaaact ctgcgtcacc ttaagtcata aaaattatcg taatattgtt
    66241 tctttaactc agaatcaatc atttccgtta gtgaatcgcc tggcagcttt tatattactc
    66301 tcgcaggaag gtgatcttta tcacgaaaag catacgcaag cggcagagta tttaggcgtt
    66361 tcttatcgac atcttttata tgttctcgcg caattcatta atgacggttt attaattaaa
    66421 agcaagaaag ggtatctcat taaaaacaga aagcagttgt caggactggc gctggagatg
    66481 gacccggaga ataaattctc cgggatgatg cagtaaaaat tattggcaat agtgcgattg
    66541 ccggatgcaa cgcttaataa ggcgtacatc cggcaatgaa actcaagcta aaccaataaa
    66601 gaacccggca atggtcgcgc tcatcaggtt ggaaagcgtc gccgccgcca gcgcccgtaa
    66661 accaagctgg gcgatttccg gcgcgcgttt tggcgaaata gccgaaaacg ctccaacgac
    66721 aacaccgata gaaccaaagt tagcaaaccc acagagggcg aacgagataa tggcaatggt
    66781 cttgacatcc agcgtgccgc tcgtttgcag atagggcgag aaattgagat aagcaacaaa
    66841 ctcattaatc gccaatttct gcccaatcaa acttccggca agatttgcat cactccagtc
    66901 cacccccata atccatgcca acggggccag cacatagccg aaaatgcttt ccagagagac
    66961 attggcaaaa ccaaaccagc cgccaactcc accaataatg ccgttgatca gcgcaatgat
    67021 cgccacaaat gccattacca ccgtcgccac accggcggcg attttcagcc cggtcattgc
    67081 accgttcgcc gcagcttcaa taatgctttt tggcggtgtt tcgctaaacg acagattttc
    67141 aaacgtaact tgcgaaggtt ccgtcgccgg actaagaata cgtgcaaaca ggataccgcc
    67201 aggaatagcc atcagcgaag cggctaacaa atagtcaatc ggcacgccca ttccggcata
    67261 gccaatcatc atcgaacccg caatagacgc catgccgcta caaatagccg taaatagctc
    67321 gttgcgattc atgcgatcaa taaaaggttt aacgatcgcc gggatctcat tttgcccgag
    67381 gaagatagtg gtgactgcga caaaagattc gattttgctg atgttgaggg ctttctgaaa
    67441 aatactgcca agaatacgaa tcagcagccc cattacgcca atgtagtaca gcaagctgat
    67501 aagcgcggta acgaaaataa tcgccgggag tacgcgaaag gcgaagataa agcccgcacc
    67561 gtcaaacaga acatccattt tgggaccgac cagcgaacca aaaataaagg cacttcctgc
    67621 gtcactgtag gacatcactt tatggacgcc taatgccgcc tgctctactg cccattttcc
    67681 cgggggaaaa tagagcatga tgccaccaat ggcaatttgc agcagcagcg cagccccaac
    67741 cgtgcgcaag ctgatgctct ttttatttac cgacaacaca aaggcaattg ccagcaatac
    67801 caccatcccc acaacacttc tcattatatc cataatgatt ttcccttcat gccagtaaac
    67861 ccggcgtcag cgccaggttt tggtatgttt gatgagtacg ggcgacggct ttctgcccac
    67921 cagaaattaa cccacgagac gctgatattc tttggcaatt tcgctcgcca gaatggcgtt
    67981 attaaagacc agttgaatat tggatttcag gctatcaccg ccggttagct ccgcaacacg
    68041 cgccagcagg aacggtgtac tctctttccc aataacaccc tgttcttcgg cttcagcaac
    68101 ggcttgatca attgctgcat tgatggtttc ttctggcatc gcaaactgtt ccgggatcgg
    68161 gttcgctacc accaggccgc cgttcaaacc ggtttgccac ttcactgcca tcgcgcgagc
    68221 aatttcgcgg gcactgtcga gacgaatgct gacatcaaat gggctggttc ggcagaaaaa
    68281 cgcaggcagc gcttttgtct gataaccaat taacggtaca ccgaaggttt ctaaatattc
    68341 tgttgttaat cctaaatcga gaatagattt tgcccccgca caaacaacag tgacattggt
    68401 atttgccagt tcctgtaaat cggcagaaat atcgaaggta tgttccgctc cgcgatgcac
    68461 accaccaata cctccggttg caaaaacttt aatcccagcc agtgctgcaa taatcatggt
    68521 tgatgcaacg gtagtcgcgc catttttccc tgcagcaacg acaaaaggta aatcacgacg
    68581 actaactttc gtcacgttat gtccttcacg gcccagcaat tcgatttctt ctttacttaa
    68641 gcccactttc atcacaccgc caataatggc gatagtggct ggtactgcgc cctgtttacg
    68701 aattgtttct tctacttcaa ttgcggtttg ggcattttgt gggaatggca ttccgtgaga
    68761 aattatggtc gattccagcg caacaaccgg ttttttattt tttaaagcat cctgtacttc
    68821 agcggatatt tgtaataatt caggggacag agttaattca gacattctgt gttctccact
    68881 aacgatgtaa cattggcaat tgataattca gggttgttgg tatattcaca ggcaagtgcc
    68941 attgacgaac atccctgcgc aaatcgaaca gaatcgataa acggcatacc gtctacccaa
    69001 caggaggcca gccccgccat catggcgtcg cctgccccag taacattgat gacattcgtt
    69061 ttaatagggg ccgaccaccc actttcaccg ttgatatcac tgtagtaaac gccatcccct
    69121 cccatgctca gcaccaggcg gttcaggccg tgttgatgaa accaggcggc gacctttgcg
    69181 acatcttcac gtccggaaag tgcaattccg ctcaacgttt cggcttcaag acggttaggt
    69241 tttaacgtat ggattttgct cagatgatcg cggattttga cgcatttcca tgccgatacc
    69301 ggatcaataa atacaggcgt ttccccgcta ttttccagaa cccagaccag cgcctcttca
    69361 ctaaggttgc aatccgcgac aataaccttt gcaccgcgaa taaactcctg atgctgggca
    69421 agaaattctg cagaaataca gtcactaata ctcatatcat ttatcgcgac aagcatttct
    69481 cctgtattat cgagcagtga taaatagctg gatgtattct cacccggaac aattaagcat
    69541 ttatcgacat aaacaccgga ttgattagtt tgcgccagta atgattgccc ataaaaatca
    69601 ccacctactg cacttaaaag ccaggcattt ttccccagta acgccagatt atgagcgata
    69661 ttccttccca cgccacctgg ggtaaattta atttttccgg gattcgaatc cgcataattc
    69721 aatggatgat gtgaataccc agccacatca atattcgccg agccaataat tactatgtag
    69781 tccttttcgc gcatagcacg ccccctggca ataaattgcc aacgaagaaa ttgggtaaaa
    69841 tagtcattac agtcaataag actattagag catgtgttca gatttaaaca catgttcata
    69901 ataagcatgt ttaacgtaat gtaaatcgat cagaaaaaac gaaatcggcc agatatgaag
    69961 gcggtcacaa aatttgtcta tttatgcagc aacaaaagag aaagttgtga tgtgtaacac
    70021 acaatatggc aggcaagaaa aagggcagag atgtttctgc cctcagattg ttgacaatgc
    70081 gcgctttgtt catgctggat gtggcgtaaa cgccttatcc gacctacaaa agcgcaaaaa
    70141 ttcaatatat tgcagggttc atgtaggtct gataagcata gcgcatcagg caattttgcg
    70201 tttgtcatca tcggggcaga gatgtctcat ccccgatgat gaagagaaat tatgcgtttt
    70261 tttctactat ttgtgtttcc gggcgtttga ggaaggcata ggccaaaccc gccaccagcg
    70321 taccggcaat aattgctacc aggtaaccca ataccggcgt aatagcgcca gggatcagca
    70381 gaacaaacag accaccgtgc ggagccatca gtttcgcacc aatcgccatt gagattgcgc
    70441 cagtcagcgc cccaccaacg atacagcacg gtagcacacg catcggatca cgagcagcaa
    70501 acggaattgc accttccgaa ataaagcaca gaccaagtac cagagcggct ttgccacctt
    70561 cctgctgcgc tttatcgaat ttacgacgcg ccaccattgt tgccagaccc attgccagcg
    70621 gtggcaccat acctgccgcc ataatcgccg ccatcgggcc ataggtttga gtactcagca
    70681 gacccacacc gaatgcgtac gctgctttgt ttaccggacc gcccatgtca gtacacatca
    70741 tgccaccgag gatcgccccc agcagaaccg cattcgcagt ccccatggtc tgcagccagt
    70801 gagtcaaccc atcgagaatg ccagcaaccg gtttaccgat taggtagatc atcgccagac
    70861 cgaccaccag actggaaatt agcggaatga tcaggatcgg tttcagtgcc tccatactct
    70921 gtggcagttt cagttgcgta ctgattaact tcgcaatgta acccgccagg aagcccgcaa
    70981 taataccgcc aatgaagcca gaaccggtgc taaccgccag cataccgcca atcagacccg
    71041 gcgtgagacc cggacgatcg gcaatggaga aggcaatata acctgccagt accggcacca
    71101 tcagcgcaaa ggctgaacca ccaccaatct gcatcagcgc cgctgccaac gtacccggct
    71161 ctttaaacgc ttcgatacca aaagcaaaag aaagcgcgat acacagacca cctgcaacaa
    71221 ccatcggcag catgtaagag acgcccgtca gcaagtgacg gtaagcgcct gcactctctt
    71281 tcttaccttc agtggtcgct gtttgagctt tgcccgctgg ttcatacggc gttgcttcag
    71341 caaccgcttt atccagttcc tgcgcagttt tcttcagcgc cagaccggta gaggtgcgat
    71401 acatcggttt accagcaaat ttcgccagat ccacttcgat atctgccgcc acaatcacca
    71461 gatccgctgc tgcgacttct tcaggtgtga ttgcattacc cgcgccaaca gaaccacggg
    71521 tttcaacttt cacccaccag ccacgttttt tcgcttcggt ttcaatggct tcagccgcca
    71581 taaaggtgtg tgctacacca gtcgggcaag cagtcaccgc aactacgcgt ttcggaccgc
    71641 tagcagcaac cggtgctgtc gcagcgaccg gcgcagtgta aggtttcgca tggcctttgg
    71701 cttcactcag gaacagctca gggtgcgcaa ctgcccggga aatatcgccc agccagacat
    71761 ttttaccgtt cagcgcgctg tcattcggga tgaaatcacc gagaacaatc gccatttctg
    71821 cgtcgttcgg attgtcgatg atttccagtt ttgcttttcg cgccgccgcg cccagcaggg
    71881 tcttcgccat ataggcgcgt gcctgaccga gattagcgtc aataatcagc agcgttttca
    71941 ttatgcctct cctgctgtca gttaaaaggt tgtaagtcga cgcgcgccat cattgcggcc
    72001 aactgcggac gatcggtaat acccacattg ctttgactta ccgccagggc tgcaacagct
    72061 gtcgccagac gcagtgtgtg ttcactggat tcacgcatca gcaagccata aatcaggcca
    72121 ccaaccatag aatcccctgc gccaacggtg cttacgacat cgactgacgg tggtttggcg
    72181 atccattcgc cggaggcatt aacccaaagc gcgccttcgg cacccagtga aataacaaca
    72241 tgcgcaatac cttgttcacg tagcgcatgt gcagcttcaa tcacatcttt catttcaggc
    72301 agtttacggc ctgcccagat ttccagctcg cggcggttag gtttcaccag ccacggtgcc
    72361 gctttcaaac ctgctactaa cgcttcacgg ctactatcaa agataatgca aggacactga
    72421 ctacgcaggc gagtcatcca gtcggtgaac gcttccgggc tgacgcctga cggtaagctt
    72481 ccgctgacac agaccatatc gaactgaccg agccagctca gagaatcagt cacaaagcgt
    72541 tcccagtcgg cgggggtaac ttcaaaaccc gagaagttga agtcggtcac ttcgccgtct
    72601 ttttccgtca gcttaacgtt aattcgggtg cgcccctgta caacctggaa acggttggca
    72661 atgcccagct cgctgaacag ttgctgaaaa ccatcctgat tgtctttacc caggaagccg
    72721 ccaacggtga catcaattcc caggtctttt aatactttgg ccacgttgat gcctttaccc
    72781 gccgcatgca gaccggtggt tttcaccagg ttcacttcgc cgcgttcaat ttccgggcag
    72841 aaaccaacaa ggtcataagc cggattaagg gtgatcgtag caacacgtct gctcattatg
    72901 cgccctcccc aagaccagca gcgatagcgt cgccaatggc tttcagcgcc tgttcagcat
    72961 ctgcaccctg ggcggtaaag cgtaggcgat gacctttctt aacgccaagt gccacaactt
    73021 tcatcagact acgtccgttt gccggtttgc cggtgccatc aaggtttgtc acggtaatat
    73081 cactgttaaa ttgtttaatg gtattgacca gcatggtacc tggacgagca tgcaggccgt
    73141 gttcattgcg caccacaaac tccgcgctta acacgtcgtc ggtcggcgca tcatcgctgg
    73201 tcagcagcgc cagcaacgtt gccgcatccg ctttcagcaa gcggtcagct ttattatcga
    73261 gcaacaaatc agcgagacgc ttaagaaccg cgatgggctg atcgtcattc attgccacac
    73321 tcaccagcat ggctgccgtt tcgccgtcca catcaaaagc atttgccgca cggcttaccg
    73381 caatcgcgct acgcagattg ccttcggcgc tatcgctcag ccagataccc tgtccgaggt
    73441 tcagcggttg ttcattgatg gctttggtga cgaaagtggc gtcaactgcc cccgcttctt
    73501 tcagacgcgc agcgttcagc gcctgaagag tcagcagatc gctggcgacg atatccagcg
    73561 tcagcatttc gttgtcgagc ttcagctgct cactctgctt ttcgcccatc agcaatgcgc
    73621 gaagttcttc tgctgttgtt gctgacttca gttgttcagc aacggaatca tcgctcagta
    73681 cgtgggtcag ctggcgtagc aggcccagat gttcatccga gctggcagca ataccgatcg
    73741 ccacgtacgc tacctggccg tccccccagg tgacgccttc cgggaactga aatacctgaa
    73801 cgccggtttt cagcacctga tcgcgggtgt cggtagtgcc gtgtggaata gcaataccat
    73861 tgccgaggaa cgttgaggtt tgctgttcgc gcgccagcat gccattgacg tagccttcag
    73921 ctacattacc ggcctgcacc agcgccgcag cgacctggcg aatcgcctct tctttgtctc
    73981 cggccttttc gcccggatgg atgtcctgta cagataactg gaacatagtt ctcctctctt
    74041 gctgaattga aacgattcag cttctatgag aaaaaaagcg ccaacctggc ttagggttaa
    74101 agacaagatc gcgctgaaac gtttcaagga agcataatac ttctgtttca gcgcgcaagg
    74161 aaagccgtaa agttatgagc aaaagtttga tgtgctgcac aattttttgt catttttcct
    74221 aattgctgat gggaaaatcc gttatcagca atttcatttc agcagtgtca gcagccaatc
    74281 agcaacagtg aaatggcatt ttgattattc gtggcgcttt tgatttcgct gtctgtttat
    74341 tttctgacaa gcagcgtaaa ctccgcgtct tcctcttcca gtgatcgacc agcatgcata
    74401 actcccccac agtcaccagc gcgaaatcgt ttgacctgac ctcgacggcg tttttaatcg
    74461 ttgcctttct caccggtatt gcgggcgctc tgcaaacccc gacactcagt atttttctta
    74521 ctgatgaagt acatgcccgt ccggcgatgg tgggattctt ctttaccggc agcgctgtca
    74581 ttgggattct ggtgagtcag tttctcgccg ggcgctctga taagcgcggc gatcgcaaat
    74641 cgctgattgt cttttgctgc ctgttaggcg tgctggcctg cacccttttt gcctggaatc
    74701 gcaactactt tgttttgcta ttcgttggcg tctttcttag cagctttggc tcgaccgcta
    74761 acccgcaaat gtttgccctt gcccgtgaac atgctgacaa aaccggacgt gaggcggtga
    74821 tgttcagctc ttttttacgc gctcaggttt cactggcatg ggtcattggc ccaccgctgg
    74881 cttatgcctt agcgatgggt ttcagcttta cggtaatgta tctgagcgca gcggtagcgt
    74941 ttattgtttg cggcgtgatg gtgtggctgt ttttgccgtc gatgcaaaaa gagcttccgc
    75001 tggcgaccgg cacggttgaa gcgccgcgcc gtaaccgtcg cgatacgttg ctgctgtttg
    75061 tcatttgtac attgatgtgg ggctcgaaca gcctgtacat catcaacatg ccgctattta
    75121 ttatcaacga actgcatctt cccgagaaac tggccggtgt gatgatgggg accgccgccg
    75181 ggctggaaat cccgaccatg ttgatcgccg gatattttgc caaacgtctg ggtaagcgct
    75241 tcttaatgcg cgttgctgcc gtgggtggcg tctgttttta cgcaggaatg ctgatggcgc
    75301 attcacctgt cattctgttg ggcttgcagc tgctaaatgc tatttttatt ggcattcttg
    75361 gcggcatcgg gatgctctat tttcaggatt tgatgcccgg tcaggcaggt tcagccacca
    75421 cgctctatac caacacttcg cgcgtgggct ggatcatcgc aggatcagtg gcgggcatcg
    75481 tcgccgagat ctggaattat cacgctgtgt tctggtttgc gatggtgatg attatcgcca
    75541 ctctgttttg cttactgcga attaaagatg tttaaggcgc ggtcaacatt tccagatcga
    75601 taagccagat catggcctgc tgacgcgagt taccgcacat ttcagcgctg gcttgtaagc
    75661 ctgcacagac tttcggtcgc agtggcgagg tgaaaatttt acagcgttgc tgttcatcaa
    75721 gctgaatgca gggagtattg gcgggcttgc catctggcat accgggaata gggctggaaa
    75781 ttgaaggggc agtgcaacac gccccgcaac ccggacggca ttccatacgt tgttctctta
    75841 aggttaacca ccggattgat gagcgcgcac agtaccacct tttttgcacc agcaaaagtg
    75901 cgaataccac ttgcccgaaa ggcccgtcgc gagtactttg tcgcgatatt tttgacattt
    75961 ttgactacag gaatttttcg atgccaagag cgaacgaaat taaaaaaggt atggtactga
    76021 attacaacgg caaactgctg ctggttaagg acattgatat tcagtcgccc actgcccgcg
    76081 gtgccgctac gctgtacaaa atgcgttttt ctgatgtccg taccgggctg aaagtagaag
    76141 agcgtttcaa aggtgatgat atcgttgaca ccgtgacgct gacccgccgt tacgttgact
    76201 tctcctatgt cgatggcaac gaatatgtct ttatggataa agaagactat accccgtata
    76261 ccttcaccaa agatcagatt gaagaagagt tgctgtttat gcctgagggc ggcatgccgg
    76321 acatgcaggt gctgacctgg gacggtcagt tgctggcgct tgagctgccg cagaccgttg
    76381 atctggaaat cgttgaaact gcaccaggta tcaaaggggc atctgccagc gcccgtaaca
    76441 aaccggcgac attgagcact ggtctggtga ttcaggtacc agaatactta agcccgggcg
    76501 aaaaaattcg tatccatatc gaagaacgcc gttatatggg tcgtgctgac taacttcagc
    76561 cgcatgcaga aaagggatag ctcaggctgt ccctttttta atttattata ccaatcttct
    76621 attttgcgct tcctgaacac cttacgcccg caattttcac tcgttgattg atcgccctca
    76681 cacttcatcg cattaacaat ccagaccaat ttcaattgct gtcatataac tttacactgt
    76741 cattgttaat taatcgttac taagacgtga ctcctatgaa tacaatcgcc tccgttacgc
    76801 tcccgcatca tgtacacgct ccacgctacg atcgacagca gttgcaatca cgtatcgttc
    76861 attttggctt tggagccttt caccgcgcac atcaggcatt actgaccgat cgtgtgctga
    76921 atgcccaggg cggcgactgg gggatctgtg aaatcagctt gttcagcggc gatcaactga
    76981 tgagccagct ccgcgcacag aaccatttat ataccgtgct ggagaaaggt gcggacggca
    77041 atcaggcgat aattgtcggt gccgttcacg aatgtctgaa tgcaaaactg gattccttag
    77101 cggcaattat tgagaaattt tgcgagccac aggtggcaat tgtttccctg acaattaccg
    77161 aaaaaggcta ttgtattgac ccggccaccg gtgcactcga caccagcaat ccgcgtatta
    77221 ttcacgatct acaaaaccct gaagaacctc actccgcgcc aggtattctc gtcgaagcac
    77281 tgaaacgccg ccgtgagcgc ggccatacac cgtttaccgt gctctcctgc gacaatattc
    77341 ccgacaatgg ccatgtggtg aaaaacgcgg tgctgggaat ggcagaaaaa cgttcgccag
    77401 aactcgccgg gtggataaaa gaacacgtca gttttccggg aaccatggtc gaccgcattg
    77461 ttccggctgc aacaaacgaa tcactggcgg aaataagcca gcatctgggg gtgaatgatc
    77521 cctgcgcgat tagctgcgaa ccgtttatcc agtgggtggt tgaagataac ttcgtcgctg
    77581 ggcgtcctgc ctgggaagtc gcaggtgtac aaatggtgaa tgatgtcctg ccatgggaag
    77641 agatgaaact gcggatgctt aatggcagcc actcttttct cgcttatctg ggttacctct
    77701 caggattcgc ccatatcagt gattgcatgc aggatcgcgc atttcgccat gccgccagaa
    77761 cattaatgct ggatgagcaa gcgccgacac tgcggattaa agatgtcgat ttaacacaat
    77821 atgcggataa gttaattgca cgttttgcta atccggcact gaaacataag acctggcaaa
    77881 tcgcgatgga tggcagccag aaattaccgc aacgcatgtt ggcaggtatt cgcatacatc
    77941 tggggcgcga aacggactgg tcgttgctgg cattaggcgt tgcaggctgg atgcgttacg
    78001 tcagcggcgt tgatgatgcc ggaaatgcca ttgatgttcg cgatccgctt agcgataaaa
    78061 ttcgtgaact tgttgcaggc agcagcagtg aacaacgcgt aaccgccctg ctttccctgc
    78121 gtgaaatttt cggtgatgat ctgccagata acccgcattt tgtgcaggtc atcgaacaag
    78181 cctggcaaca aatcgcacaa ttcggcgcgc atcaggcgct attaaacacc ctcaaaattt
    78241 aacgatttct gcggttaaag cggatgaagc tcgccttcgt ccgctctccc cttctctttt
    78301 ctgccttttt tagccaggat taacgctcag ttaacttacc agagtattgc ggagccgttg
    78361 tgaccagaac caacctcatc accggttttc tcggcagcgg gaaaactacg tcgattcttc
    78421 atctgttagc ccataaagat cccaacgaaa aatgggcggt actggttaat gaatttgggg
    78481 aagtcggaat tgatggtgct ttgctcgccg atagcggcgc actgctgaaa gagatccccg
    78541 gcggttgcat gtgctgcgtt aatggtttac ccatgcaggt agggttgaat accttactgc
    78601 gtcagggaaa accagaccgc ttgttgatag agccgaccgg gctgggccat ccgaaacaga
    78661 tcctcgatct cttaaccgcg ccagtctatg aaccgtggat agatctgcgc gccacgttgt
    78721 gcattctcga tccacgcctg ctgctggacg aaaaaagcgc cagcaatgaa aacttccgtg
    78781 accagctggc tgccgcagac attattgtcg ccaataaatc cgaccgtgcg acgcccgaaa
    78841 gtgagcaagc gctagagcgc tggtggcagc aaaatggtgg cgatcggcaa ttaattcaca
    78901 gcgagcatgg aaaaattgac ggtcatcttc tggatttgcc gcgtcgcaat ttagccgagt
    78961 tgcccgccag cgccgcgcat tctcatcagc atagcgtgaa aaaagggtta gcagcgttaa
    79021 gcctgccaga gcatcaacgc tggcgtcgca gtctgaacag cggacaaggg catcaggcct
    79081 gtggctggat attcgacgct gatacggtat tcgacaccat tggtattctg gaatgggcgc
    79141 gacttgcacc ggtggaacgc gtcaaaggcg tgctgcgtat tcccgaaggg ctggtgcgaa
    79201 tcaaccgtca gggcgatgac ctgcacattg aaacgcaaaa cgttgcgcca ccggacagtc
    79261 gtattgagct gatttccagc agcgaagctg actggaatgc cttgcagagc gcgctgttga
    79321 agcttcgttt agcgactacc gcgtaaggtt gcctgcgttt ttcagtaaga taattagaga
    79381 aaatatgatt aaaaatttgc cgcaaatagt gttgttgaat attgtcggcc tcgcgctgtt
    79441 tctttcctgg tatattccca ttaatcatgg attctggttg ccgattgatg cggatatttt
    79501 ttatttcttt aatcagaaac tggtcgaaag taaggccttt ttgtggctgg ttgcattgac
    79561 caacaatcgc gctttcgacg gttgttcact gctggcgatg ggaatgttga tgctgagttt
    79621 ctggctgaaa gaaaacgccc caggcagacg acgtatcgtg attattggtc tggtcatgct
    79681 attaactgca gtggtattaa accagctggg tcaggcatta attcctgtaa aacgggccag
    79741 tccaacgttg acttttacca atattaaccg cgtcagcgaa ctgctctctg ttcccacgaa
    79801 agatgcctca cgagatagct ttcccggcga tcacggtatg atgctgctta ttttttcggc
    79861 attcatgtgg cgttatttcg gcaaagttgc tggccttatc gcccttatta tttttgtggt
    79921 ttttgcgttt cccagagtaa tgattggcgc acactggttt actgacatca tcgtcggttc
    79981 catgaccgtg atattgatcg gtttgccctg ggtgttgctg acgccattaa gtgatcgatt
    80041 aatcaccttt tttgacaaat cactacctgg aaaaaacaaa catttccaaa acaaataact
    80101 cacagtaatt aacatcatca ggtttatttt tatagtgagg ataatcctga tgatgcgcct
    80161 cctgctttca tccatcgagc gcaaaaatca ttctctaagt aaatgaatgg attgcatgcg
    80221 tttcactcaa tcgtacttta attgaccaat cccgctgatt aactttctgt atcacttttt
    80281 cttataaaaa atcatgtaaa accgctcgcc aagaccgcac caatcgggta atctcgaact
    80341 cgttttgcct cggcggtaga ttatcctcac agcatataat tttgtgcgtt agtccacaga
    80401 tttggcctta aggaattgtt tcaacatgcc caggtaatta gtctcgtgtc gcttggcatt
    80461 tttttataac gatatttgtc gttaaggact tcaagggaaa acaaacaaca tggtcaaatc
    80521 tcaaccgatt ttgagatata tcttgcgcgg gatccccgcg attgcagtag cggttctgct
    80581 ttctgcatgt agtgcaaata acaccgcaaa gaatatgcat cctgagacac gtgcagtggg
    80641 tagtgaaaca tcatcactgc aagcttctca ggatgaattt gaaaacctgg ttcgtaatgt
    80701 cgacgtaaaa tcgcgaatta tggatcagta tgctgactgg aaaggcgtac gctatcgtct
    80761 gggcggcagc actaaaaaag gtatcgattg ttctggtttc gtacagcgta cattccgtga
    80821 gcaatttggc ttagaacttc cgcgttcgac ttatgaacag caggaaatgg gtaaatctgt
    80881 ttcccgcagt aatttgcgta cgggtgattt agttctgttc cgtgccggtt caacgggacg
    80941 ccatgtcggt atttatatcg gcaacaacca gtttgtccat gcttccacca gcagtggtgt
    81001 tattatttcc agcatgaatg aaccatactg gaagaagcgt tacaacgaag cacgccgggt
    81061 tctcagccgc agctaataaa ccgtttggat gcaatccctt ggctatcctg acgagttaac
    81121 tgaaagcact gcttaggcag tgcttttttg ttttcattca tcagagaaaa tgatgtttcc
    81181 gcgtcttgat ccaggctata gtccggtcat tgttatcttt taaatgttgt cgtaatttca
    81241 ggaaattaac ggaatcatgt ttatacgcgc tcccaattct ggacgtaagc tcctgcttac
    81301 ctgcattgtt gcaggcgtga tgattgcgat actggtgagc tgccttcagt ttttagtggc
    81361 ctggcataag cacgaagtca aatacgacac actgattacc gacgtacaaa agtatctcga
    81421 tacctatttt gccgacctta aatccactac tgaccggctc cagccgctga ccttagatac
    81481 ctgccagcag gctaaccccg aactgaccgc ccgcgcggcg tttagcatga atgttcgtac
    81541 gtttgtgctg gtaaaagata aaaaaacatt ctgttcatct gcaactggtg agatggacat
    81601 tccactcaat gaattgattc cggcgctcga cattaataaa aacgtcgata tggcgatctt
    81661 acccggcacg ccgatggtgc cgaacaaacc cgcaatcgtc atctggtatc gcaacccttt
    81721 gctgaaaaat agcggcgtgt ttgccgctct gaatctcaac ctgacgcctt cactctttta
    81781 tagttcacgg caggaagatt acgatggcct cgccctcatt attggtaata ctgcgctatc
    81841 taccttttct tcacgtttga tgaacgttaa tgaattaacc gacatgccgg tccgtgaaac
    81901 taaaattgcg ggcattcctc tgaccgttcg gctttatgca gatgactgga catggaacga
    81961 tgtgtggtac gcatttttac tgggtggcat gagtggaact ttcgttggac ttctctgcta
    82021 ttacctgatg agtgtgcgta tgcgcccagg cagagaaatc atgaccgcca tcaagcgcga
    82081 acaattttac gtggtatatc aaccggtggt tgatacacaa gctttgcggg taacgggcct
    82141 ggaagtactg ctacgctggc ggcatcctgt agcaggagaa atacccccgg atgccttcat
    82201 taactttgcc gaagcgcaaa agatgattgt gccactgact cagcacctgt ttgagttgat
    82261 tgcccgcgat gccgcagaat tagaaaaagt actgccggta ggcgtcaaat ttggcattaa
    82321 cattgcgccg gcccacttgc acagcgaaag ctttaaagcg gatatccaga aactgctcac
    82381 ttccctgccc gcacaccatt tccagattgt gctggaaatt accgagcgcg atatgctgaa
    82441 agagcgagaa gccacacaac tcttcgcctg gctgcattcg gtcggcgtag aaatcgctat
    82501 tgatgacttc ggcaccgggc acagcgcgct tatctatctt gagcgtttta cgctcgatta
    82561 tctgaaaatt gatcgtggat ttatcaacgc catcggtacg gaaacgatca cttcacccgt
    82621 acttgacgcg gtgctgacgc tggcgaaacg tctcaatatg ctgacagttg ctgaaggggt
    82681 cgaaacgcca gaacaggcac gatggctaag cgaacgcggc gttaatttca tgcaaggcta
    82741 ctggattagt cgcccgttac cgctggacga ttttgttcgc tggctgaaga aaccgtatac
    82801 gccgcagtgg taaggtgtgc ttacgtccct tattattcat agtgaaagca tgccggattg
    82861 cggctaatga tgagtaaaag gaaatccgtt gcagatgatt gtgcgcatac tgctgctgtt
    82921 tatcgctctg ttcacctttg gtgcgcaggc gcaggctatc aaggaaagct atgcctttgc
    82981 agtactgggc gaaccccggt acgcatttaa tttcaaccat tttgattatg tgaaccccgc
    83041 cgcgccaaaa ggtgggcaaa taacgttgtc tgccctcggc accttcgata atttcaaccg
    83101 ctatgcactg cgcggcaatc cgggcgcacg caccgagcag ctgtacgaca cgctatttac
    83161 gacttccgat gacgaaccag gcagttatta cccgctgatt gctgaaagcg cacgctatgc
    83221 tgacgattat tcctgggtgg aggtcgctat taatccacgc gcccgttttc atgatggttc
    83281 gcccattact gcccgcgatg tagagtttac ttttcaaaaa tttatgaccg aaggcgtgcc
    83341 gcaatttcgt ctggtctaca aaggcaccac cgtcaaagcc attgcgccgt taaccgtgcg
    83401 cattgagtta gctaaacccg gcaaagaaga tatgctgagt ctgttttcgc tgccggtatt
    83461 tccagaaaag tactggaaag atcacaaaat tagcgacccg ctcgccacgc ctccgcttgc
    83521 cagtggtccg taccgcatta cgtcctggaa aatggggcaa aatattgtct attcccgcgt
    83581 gaaagattac tgggcagcaa acttaccggt aaaccgtgga cgctggaatt tcgacaccat
    83641 tcgctacgat tattacctcg atgataatgt cgcctttgaa gcgtttaaag caggagcctt
    83701 tgatttgcgt atggaaaacg acgccaaaaa ctgggccacg cgctataccg gtaaaaattt
    83761 cgataaaaaa tacatcatca aagatgagca aaagaacgaa tcagcccagg atacgcgctg
    83821 gctggcgttt aatatccaac gtccggtatt cagcgatcgc cgggtgcggg aagcgatcac
    83881 tctcgccttt gactttgaat ggatgaacaa ggcgttgttt tacaatacct ggagtcgcac
    83941 gaacagttat tttcagaata ccgaatatgc ggccagaaat taccccgacg ccgcggaact
    84001 ggtgcttctg gcaccaatga aaaaagatct accgccagaa gtcttcacgc aaatctacca
    84061 gccgccggta tccaaaggcg atggctacga tcgtgacaac ctgttaaaag ccgacaaact
    84121 tctcaacgaa gcaggctggg tgctgaaggg tcagcaacgc gttaatgcca caacaggtca
    84181 gccactcagc tttgaattat tgcttcccgc aagcagcaat agtcagtggg tattgccgtt
    84241 ccagcacagc ctgcaacggc tgggtatcaa catggacatt cgcaaggtgg ataactcgca
    84301 aatcaccaac cgcatgcgca gtcgcgacta tgacatgatg ccgcgcgtat ggcgggcgat
    84361 gccgtggccc agttccgatt tacagatttc ctggtcatcg gaatatatca attccactta
    84421 taatgccccc ggcgtgcaaa gcccggttat cgactcgctg atcaaccaaa ttattgccgc
    84481 gcagggaaat aaagaaaaat tactgccgtt gggacgagca ctggatcgcg tattaacgtg
    84541 gaattattac atgctgccaa tgtggtacat ggcggaagac cgtctcgcct ggtgggataa
    84601 attctcccaa cccgctgtac gcccagttta cagcctgggt attgatacct ggtggtatga
    84661 cgttaataaa gcgacgaaac tgccgtcagc cagacaacag ggagagtaga tgggcgctta
    84721 tctgatccgc cgtctgttgc tggtgatccc aacgctatgg gcgattatca ctatcaactt
    84781 tttcatcgtg caaattgcgc ctggcggtcc ggtcgaccag gccatcgccg ccattgagtt
    84841 tggcaatgcc ggagtattac ccggcgcagg cggtgaaggt gttcgcgcca gccatgcgca
    84901 aacgggcgtc ggcaatatca gcgacagtaa ttaccgtggc ggacgcggat tagatccaga
    84961 agtgatcgcc gagatcactc atcgctacgg tttcgataag ccgatccacg aacgttactt
    85021 caaaatgctc tgggactaca tccgttttga ttttggcgac agcctttttc gcagcgcctc
    85081 ggtgctgacg ctgattaaag acagtctgcc ggtctccatc accctcggat tgtggagcac
    85141 gctgattatc tatctggtgt cgattccgtt aggcattcgc aaagctgttt ataatgggag
    85201 ccgttttgac gtctggagta gtgcatttat catcatcggc tacgccattc cggccttttt
    85261 gtttgccatc ctgctgattg tcttcttcgc gggcggcagc tatttcgatc tgttccctct
    85321 gcgcggcctg gtttccgcta actttgattc gctgccgtgg tatcagaaaa tcaccgatta
    85381 tctgtggcat atcacgctgc cggtgctggc gacagtgatt ggcggctttg cggctctgac
    85441 catgctgaca aaaaactcat tccttgatga agtgcgtaag caatacgtgg tgacggcacg
    85501 tgcgaaaggg gtaagtgaaa aaaatattct ctggaaacat gtgttccgca acgccatgct
    85561 gctggtgatt gccggttttc cggcgacgtt tatcagcatg ttttttaccg gctcgctgct
    85621 gattgaggtg atgttttcac tcaatggtct tggcttactg ggctacgaag cgaccgtctc
    85681 gcgcgattat cctgtaatgt ttggtacctt gtatattttc accctgattg gcctgctgct
    85741 gaatattgtc agtgatatca gctatacgct ggtcgatccg cgtatagact ttgagggacg
    85801 ttaatgtcgc gactcagccc cgtcaatcag gcccgttggg cgcgttttcg ccataaccgt
    85861 cgcggctact ggtcgttatg gattttcctc ctcttgtttg gtttgagttt gtgttctgaa
    85921 cttatcgcca acgataaacc gttgctggtg cgttatgacg gcagttggta tttcccgttg
    85981 ttgaaaaact acagcgaaag cgattttggc ggcccgctgg caagtcaggc tgattatcag
    86041 gacccgtggc tgaaacaacg actggaaaat aacggctggg tactgtgggc accgattcgc
    86101 tttggtgcta ccagtatcaa ctttgctacc gataagccct tcccttctcc accctcccgg
    86161 caaaactggc tgggaacgga tgccaatggc ggcgatgtgc tggcgcgcat tctctatggc
    86221 acgcggatct cagttctgtt tggcctgatg ctgaccctct gctccagcgt gatgggtgtg
    86281 ctggcggggg cgctacaagg ctattacggt ggtaaagtcg atctctgggg gcaacgtttt
    86341 attgaagtat ggtcggggat gccgacgctg tttttgatta ttttactttc cagcgtcgta
    86401 cagcctaact tctggtggct gctggcaatt actgtcttgt ttggctggat gagtctggtc
    86461 ggcgtggtgc gggcggagtt tttacgtacc cgtaatttcg actacatccg cgcggcgcag
    86521 gcgcttggcg tcagcgatcg cagtatcatc ctgcgtcata tgttgccaaa tgccatggtc
    86581 gctaccctca cttttttacc gtttatttta tgtagttcga taaccaccct gacctcactc
    86641 gatttcctcg gcttcggtct gccgctcggt tcaccgtcac tcggcgaact cctgttacaa
    86701 gggaaaaata accttcaggc cccgtggctt gggatcaccg ccttcttatc ggtggcgata
    86761 ttgttgtctt tgctgatctt tattggtgaa gccgtccgcg acgcatttga tcctaataag
    86821 gcggtgtagc atgacgcaaa ctctgttagc gattgaaaat ttgtcggtgg gttttcgcca
    86881 tcagcaaact gttcgtaccg tagtgaatga tgtgtcacta cacattgagg ctggcgaaac
    86941 gctggcgctg gtaggtgagt ctggttcagg taagagcgtt accgcgctgt caattttacg
    87001 cctgctccct tgcccgccgg ttgaatatct ctccggcgat attcgttttc atggcgaatc
    87061 gctgcttcat gccagcgatc aaacgttgcg cggtgtacgc ggtaataaga tcgccatgat
    87121 ttttcaggag ccgatggtgt cgttaaatcc attacatacc ctggaaaaac agctttatga
    87181 agtgctttca ctccaccgcg ggatgcgtcg ggaagcggct cgtggcgaaa ttcttaactg
    87241 ccttgatcgc gtcggtatcc gccaggcggc aaaacggctg acagattatc cgcatcagct
    87301 ctccggcggc gaacgccagc gggtgatgat tgcgatggcg ctgttaacgc gaccggaatt
    87361 attaattgcc gatgaaccaa ccaccgcgct ggacgtctct gtccaggcgc agattttaca
    87421 gctgttgcgc gaactgcaag gcgagctgaa tatgggtatg ctgtttatta ctcataacct
    87481 cagcattgtt agaaaactgg cccaccgcgt ggcggtaatg caaaacggtc gctgtgtcga
    87541 gaaaaataac gccgctacgc tatttgcctc acccactcat ccttacacac aaaagctact
    87601 caacagtgaa ccatctggcg acccggtgcc attgccagaa cctgcctcaa cgttgctgga
    87661 tgttgaacaa cttcaggttg ccttccccat tcgcaaaggg attttgaagc gcattgtgga
    87721 tcataatgtg gtggtgaaaa acatcagttt tacgctacgg gcgggtgaaa cactgggttt
    87781 agtgggcgag tccggttccg ggaaaagcac gacgggactg gcgctgctgc gactgattaa
    87841 ctctcatggc agcatcgtct ttgacggtca gccactgcaa aatttaaatc gccgccagct
    87901 gttacctatt cgtcatcgca ttcaggtggt atttcaggat ccaaactcct cactcaaccc
    87961 acgactcaac gttttgcaaa ttattgagga aggcttacgg gttcaccagc cgacgctttc
    88021 tgccgcacaa cgcgaacaac aagtgatagc cgtgatgcat gaagtgggat tagatcctga
    88081 aacacgccac cgttatccgg cggagttctc tggtggtcag cggcaacgta ttgcgattgc
    88141 cagggcgtta attcttaagc cctcgctgat cattcttgat gaaccaacat catcactcga
    88201 caaaacggtg caggcgcaaa tattgacgct attgaaatca ttgcaacaaa agcatcaact
    88261 ggcctatttg tttatcagcc acgatttgca cgttgtccgc gcgttatgtc atcaggttat
    88321 cgtactgcga caaggggaag tagtggaaca aggaccgtgc gcgcgcgtgt ttgccgcacc
    88381 gcagcaggag tatacgcgtc agctactggc gttgagctga cgcttaaaaa ggattgtagt
    88441 ttgaaaaggg ttcggcaatt gccacaccaa aatttttcaa tcggcatgtt gcagcaaatt
    88501 cgtcctggcg atttacaaac aggcacggct caccttcaca ctccagcacc gaacatggaa
    88561 cttcgatgtc gcttaacgcc tggcttagtt tgtacatcac cgaccatgca ttatcaccat
    88621 ccgggctaag caatttaagc gccaccaggc tgttgtcacc gcacggtcca ttttgcggaa
    88681 tcggttcaac cttcgaacct gcgaaaccag cagaccagcg atagttctgc ggcagtcgat
    88741 ggacgattga gagttgtaat gaagtcactt attttccccg gaagtacatt ttacttcaca
    88801 atttatttac atcaatttta acacatcatc aacaatccgt ctttatacag atcgtaaaga
    88861 ttgatgcctc gcctgcgtgg cctatatggc tctgatttaa ataatatctg cgggcgtggt
    88921 cacgtttgcg gggctatatg tacccgtttc tgcgatctaa ctcaaccttt ttaactacaa
    88981 tgatgtgact ttttacataa attgatttta cataaaataa acatatatcg ggggaatgat
    89041 agatttgtgg ttgacatgca tcaacaaagg aagcctttta gcttcctcgt tgtgcaatag
    89101 atcaccgttt tttcggccga ctggcgtaca gacagaagag aatggagctg gttgcacaga
    89161 atgcaattga ccaaatcatc ggccatgcag agttaaaggt cgcaagagaa agtaatgcgc
    89221 caacaattgc ccctatgccg aaacggaagg ttcctgccag cgaagatgcc gttcccgcca
    89281 tatggggaaa ctcatcaaga atgaccgcca tcgcattgga tgacaccatc gacacgcagc
    89341 ccacaaacgc cgcaacgcca accaccagcg accagaatcc cagccccagc agtgcactga
    89401 tgaccatcca cgctgccata ataaactgta tccacaaccc cgagcggaac atatttaacg
    89461 cgccaatacg gcggacgaag cggctgttaa agatggtcat cacgaacaga aaaagaatgt
    89521 ttagcgcaaa gtaataacca aagttttccg gcgcgacgtg gttgatttca atgtagacaa
    89581 acggtccggc gcttaagaat gagaacatcc cggcaaagct gaaaccgctg gccagcatat
    89641 aactcaggac acgcttatga cggaacagcg ccgcaaagtt accaatagtg gtacgaatgt
    89701 gaaaaggctg gcgacgctcc ggtggtaagg tttctttgat cagaaagaag atcattgccg
    89761 aagccagaat cgccgccacg gcgaggatcc agaagatgta atgccagctc agccacacca
    89821 gcacccagcc gccaactatc ggtgccatca gcggtgcaat ggtcgtcacc agcatgacaa
    89881 acgacatcat ccgtgagaac tcttctttcg ggtagatatc acgcatcaaa gcgttgataa
    89941 ccacgctggc cgcagccgca gccagcccgt ggaagaaacg catcacaatc agctgatcga
    90001 tggtttgtgc caacgcacac gccaccgcgg cggcggcaaa caccagcgta ccgccaagaa
    90061 ccaccggctt acgcccgaag ctgtctgcca tcggcccgta gattaactgc cccaacgcaa
    90121 agcccagaat ataagtactg agggtcatct gcgtactgcc cgccggtaca ccaaactgcg
    90181 ctgaaattac cggtagcgca ggcagataca tatcaatcga cagcggcatc aacatggcca
    90241 gcaggccaag gataaaaaca atagcaaacg acgaatgctg tcgggtggtc acaacgggct
    90301 cctgaaagtc attgaaaagt tagacgacgc tggcaatttc ttcttcagtt aacggacgat
    90361 attcaccggg ggctaaatca gcatccagcg taataccgcc aatacgttca cgatgcagct
    90421 caaccacatg gttacccacg gcggcgaaca tacgtttcac ctgatgataa cgcccttcgc
    90481 tgatggtcaa acgaacctgc gttggggtaa tcacttccag caccgccggc ttagtgagat
    90541 ctttttcgtt atgcagctgc acgcctttag caaattgctc tgccgtatcg tcagctacgg
    90601 gtgattccag cgtcaccaga taggtcttct cgcaatgatg gcgcggggaa gtaatgcggt
    90661 gcgaccactg accatcatct gtcattagta ccagaccggt ggtatcgata tccagacgcc
    90721 ctgcagcgtg cagtttccac gctaccggtt catcaagaaa atagagcacc gttgggtgat
    90781 ccgggtcgtc cgtggagcaa acatagccct gaggcttatt gagcatgaag taacgtggac
    90841 cgtgttgctg cgccagcggg ttgccatcgt aagcgacatc atgttcaggg agcagtttga
    90901 acgctgcatt acggacgatt tcgccatcga cggtgacacg attgccgcgg atttcacgcc
    90961 cggcaatagc acggctaacg ccgagttgct gtgcgataaa tttatcaagt cgcatgtgtg
    91021 tgattttgcc tgtaaaaaac ggaggtcggg taatgacccg aaaatctgaa ctgttgtctg
    91081 cccagtatag cggtctaata acttccctca agggaaaaag atccgtggca tactattagc
    91141 agaataatct acctacgcga gaccatgatt tttacactcc gcccatatca gcaagaagcc
    91201 gtggatgcca cgctcaacca ttttcgtcgt cataaaaccc ctgccgtaat cgtgctgccc
    91261 accggtgcag gtaaaagcct ggtgatagcg gaactggcgc ggctggcgcg tggtcgcgtg
    91321 ctggtgctgg cacacgttaa agaactggtg gcacaaaacc atgcaaaata tcaggcgctg
    91381 gggctggaag ccgatatttt tgccgccggg ctaaagcgca aagagagcca cggtaaagtg
    91441 gtatttggca gcgtgcagtc ggtcgcccgt aatcttgatg cctttcaggg tgaattttcg
    91501 ctgttgattg tcgatgaatg ccaccgtatt ggtgacgatg aagagagcca atatcagcaa
    91561 atcctcactc acctgaccaa agtgaatccc cacttacgcc tgctggggct gactgccacg
    91621 ccttttcgac tgggcaaagg ctggatctac cagttccatt atcacggcat ggtacgcggc
    91681 gatgagaaag cccttttccg tgactgcatt tatgagctgc cgctgcgtta tatgattaaa
    91741 cacggctatc tgacgccgcc agaacggctg gatatgccag tagtgcaata cgatttcagc
    91801 cgcttgcagg cacagagtaa cggactgttc agcgaagccg atctcaaccg cgagctgaaa
    91861 aaacagcaac gcattacgcc acacatcatc agccaaatca tggagtttgc tgcaacgcgc
    91921 aaaggggtga tgattttcgc cgcgacggtc gaacacgcaa aagagatagt gggattactg
    91981 cctgccgaag atgcagcact gattactggc gacacacccg gctctgagcg cgatgtgtta
    92041 attgatgatt ttaaagccca gcgttttcgc tatctggtca acgtcgcggt actgaccacc
    92101 ggatttgacg ccccgcacgt cgatcttatc gccattctgc gccctaccga atcggtgagt
    92161 ctttaccaac aaattgtcgg gcgcggtctg cgcctggccc ccggcaagac tgattgctta
    92221 attcttgatt atgcgggtaa ccctcacgat ctctacgcgc cggaagttgg tacaccgaaa
    92281 ggcaaaagtg acaacgttcc ggtacaggtt ttctgccctg cctgcggttt tgccaacacc
    92341 ttttggggga aaacgaccgc cgacgggaca ttgattgaac actttggtcg tcgctgtcag
    92401 ggatggtttg aagatgacga cggacatcgc gaacagtgtg acttccgttt ccgttttaaa
    92461 aattgcccgc aatgtaacgc tgaaaatgat attgccgccc gccgttgccg cgagtgtgac
    92521 accattctgg ttgacccgga tgatatgtta aaagcggcgc tacgactgaa agacgcgctg
    92581 gtattacgct gtagcggcat gtctttgcag catgggcacg acgaaaaagg cgaatggttg
    92641 aaaatcacct attacgatga agacggcgcg gatgtgagtg agcgtttccg tctgcaaacg
    92701 cccgcccagc gaactgcctt cgagcagctt tttatccgcc cgcatacgcg cacaccgggc
    92761 atcccgctgc gctggatcac cgccgccgat gtcctcgccc agcaagcctt attgcgacac
    92821 ccggattttg tcgtcgcccg catgaaaggc cagtactggc aagtgcgtga aaaagtgttc
    92881 gattacgaag gtcgttttcg tcgggcgcac gaattacgcg gttaatggta gttttgattg
    92941 atgtataagg cgattgagta tagaatctcg cccgcttttg catacgcaaa gcagatcact
    93001 tacctgttgc tgggtcgcct gtagcaggat taatttaaga gagaaagaaa tgtttactat
    93061 caacgcagaa gtacgtaaag agcagggtaa gggtgcgagc cgccgcctgc gtgccgctaa
    93121 caagttcccg gcaatcatct acggtggcaa agaagcgccg ctggctgtcg aactggatca
    93181 cgacaaagtc atgaacatgc aagttaaagc tgaattctac agcgaagttc tgaccatcgt
    93241 tgttgacggt aaagaaatca aagttaaagc tcaggacgta cagcgtcacc cgtacaaacc
    93301 gaagctgctg cacatcgact tcgttcgcgc ttaattgctg aataagttgt aaaaaacccc
    93361 gctccggcgg ggttttttgt ctctgcaaat tatgcctgat tacggtattg ctattttttg
    93421 caggccagat aaggcgttca cgccgcatct ggcattcaga acacaaaacc gattaattgc
    93481 cgccagacgt ccggcgctgc aattgatcgc gcaagttcgg cggcgtgcct ttaatggtca
    93541 gggtatcggt tgctggatcc cagaagatgc gttcacctaa cagcatcgca tcaaagttaa
    93601 tagtcaagcc accgccgcta ccggcaaact tcgtcaactg acgcagtgtg ctacggtctg
    93661 ccgggaagct ctcttccagt tcgtagcctt tttcagccgc aaactccgtg aagctcactt
    93721 cgctgacgcc tgccagctct ttcgacagcg attccagttc aatctcttca cctgcctgta
    93781 gctgctcatt acagtagcta taaacctgct ggcgcacgtt ctgtcgctct gctttatcca
    93841 gttgtgcttc ggcagtgaaa tcatcgaccg cttgcaacag cccacggttt tgcgctttcg
    93901 cgtttaaccc ttcgctggca ccgaggaaat ccataaagaa atccgccact ttgcgcccta
    93961 ctcgcccttt caggaaagtg agatagcggg tcgactctgg attggtttcc cactcggtta
    94021 aatcgatacg cgcaacaata tccgcatgat tgatatcaag ataatgggtt gggttgatgt
    94081 cgagattttc gttaacacgc atactgctca ggttgctcag aactgccacc agcagatact
    94141 ccaccgccag atagcgatag tggcaaaaca gcacgaatcc accgtcagca aaaggatatt
    94201 tcgctaattc gtcacgcagg cgaccggttg ccgcgcggct aaatgccaga aagtcctctt
    94261 cgccctgacg ctgtaagcgc agcgtctgcg ccagttcact ctcttcgcta aacaagccgt
    94321 aagctttatt tttggcgcta tagacccgat gcagttctgc caccatctca acgacggttt
    94381 ccgtcggttc cagcaatgaa tcgcgcaaca ccagctcaag gttctgctca tcacgcttga
    94441 taagctggtg cagagcaatc tggttgatat ccagactcat gataaactct cctttaagac
    94501 cgggcggtat tcaaccaccg ccggttaagc gacgcaataa aagatggcgg tcattttaca
    94561 gaaggcggcg taacgagaat gatttaacgc ataaaaaagc agaaaaaaaa ccgttgctac
    94621 ggtaatatgt tgccctttca tcaactaact gatttcgatt tatgccacaa atttcccgct
    94681 acagtgatga acaggttgaa caattgctcg cagagctgct caacatactg gaaaaacata
    94741 aggctccgac cgacctttcc ctgatggtgt taggcaacat ggtcactaac cttatcaaca
    94801 ccagcattgc cccggcccaa cgccaggcaa ttgccaactc ttttgcccgc gccttacagt
    94861 cctctatcaa cgaagacaaa gcgcactaag ggaaacagat aacaggttat ggtaactcat
    94921 cgtcagcgct accgtgaaaa agtctcccag atggtcagtt gggggcactg gtttgcactg
    94981 ttcaatattc tgctttcgct cgtcattggc agccgttacc tgtttatcgc cgactggccg
    95041 acaacgcttg ctggtcgcat ttattcctac gtaagcatta tcggtcattt cagcttcctg
    95101 gtgttcgcca cctacttgct gatcctcttc ccgctaacct ttatcgtcgg ctcccagagg
    95161 ctgatgaggt ttttgtccgt cattctggca acggcgggaa tgacgctatt actgatcgac
    95221 agcgaagtct ttactcgttt ccatctccat cttaatccca tcgtctggca gctggttatc
    95281 aacccagacg aaaatgagat ggcgcgcgac tggcagctga tgttcatcag cgtgccggtt
    95341 attttattgc ttgaactggt gtttgcgacg tggagctggc aaaagctgcg cagcctgacg
    95401 cgtcgtcggc gcttcgcgcg cccgctggcc gcattcttat ttatcgcctt tatcgcctcg
    95461 catgtggttt atatctgggc cgatgccaac ttctatcgcc cgatcaccat gcagcgcgct
    95521 aacctgccgc tttcgtaccc gatgacggca cgacgttttc ttgagaagca tggcctgctt
    95581 gatgcgcaag agtatcaacg ccgtcttatt gagcaaggca atccagacgc cgtttccgtt
    95641 cagtatccgt taagcgaact gcgctatcgc gatatgggca ccgggcagaa tgtgctgttg
    95701 attactgtcg atggcctgaa ctactcacgc ttcgagaagc agatgcctgc gctggcaggt
    95761 tttgctgagc aaaatatttc gttcacgcgt catatgagct ccggcaacac tacagacaac
    95821 ggcatctttg gcctgtttta tggcatctcg ccaagctata tggacggcat tttgtcgacc
    95881 cgtacgcctg cggcgttaat tactgcgctt aatcagcaag gctatcagct ggggttattc
    95941 tcgtcagatg gctttaccag cccgctgtat cgccaggcat tgttgtcaga tttctcgatg
    96001 ccgagtgtac gcacccaatc cgacgaacag accgccacgc agtggattaa ctggctgggc
    96061 cgctacgcac aagaagataa ccgctggttc tcgtgggtct ctttcaatgg cacaaacatt
    96121 gacgacagca atcagcaggc atttgcacgg aaatatagcc gggcagcagg caatgtcgat
    96181 gaccagatca accgcgtgct caatgcactg cgtgattctg gcaaactgga caatacggtg
    96241 gtgattatca ctgccgggcg gggtattccg ctgagcgaag aggaagaaac ctttgactgg
    96301 tcccacggtc atctgcaggt gccattagtg attcactggc caggcacgcc ggcgcagcgt
    96361 attaatgcgc tgactgatca taccgatctg atgacgacgc tgatgcaacg cctgctacat
    96421 gtcagcacac ctgccagcga atattcgcaa ggtcaggatt tgttcaaccc tcaacgccgc
    96481 cattactggg ttaccgccgc ggataacgat acgctggcaa ttaccacccc gaaaaagacg
    96541 ctggtgctga acaataacgg taaataccgc acttacaact tacgtggtga aagagtgaaa
    96601 gatgaaaaac cacagttaag tttgttattg caagtactga cagacgagaa gcgttttatc
    96661 gctaactgat taattataaa tcagttagca aaatatctta cttgcaatcg gtgtggaaaa
    96721 cggtagtatt agcagccacg agtcggcacg tagcgcagcc tggtagcgca ccgtcatggg
    96781 gtgtcggggg tcggaggttc aaatcctctc gtgccgacca aaaatcccaa gaaaaccaac
    96841 ctattgcggt tggttttttt atatctgcat ttaattcgat aaacagaccg cgacacatca
    96901 cggcctgttt attttctgtt atcagaacgt ccagaccaca cccgcctgag ttgtgctgct
    96961 ttcttcacgg gagaaacggc tgtcgatacc aagcgttagc cccgtcaatg tttgctcaaa
    97021 gccagcgcct gcatcagtgg tcacgttgtt gcggctgtta attgccgaac tccacatcgc
    97081 cgtatcgtaa ttaacgcctt ttacgctacc aagacgccca cgcacggtgt ccagttctgc
    97141 atcaaatacc agcggttgtg ctgccgccat attcagcaca tccgtggttg aagggggcat
    97201 ttgtgcgcga ttctctgcga gactccagct atggttgcca ttatccagca aggtatattc
    97261 atacgtactg atatcaacaa cgcctccggc attgcccaac gtaaatgccg catcaccgcg
    97321 cccgttgtta ccagtgtaag gttatctcct gctgccgggc tggcaccggt gtccgtcacg
    97381 aatattttga aatcacctgt tgctttaacc tccagtagcc ctcccttttc tataacggca
    97441 aagacggcaa tcccatcttt ttcaacaatt aagcgccctc ctttaatcgt gggatcagtc
    97501 gccgcgccat aaacctgtag cactccatta ttgatcgtag tctggtttac cgcgccaccc
    97561 atttgaacaa tttgataacc atcatttaat actgagtatt ctgcggtgcc atttatttca
    97621 atgtactgat atcccccgtt aatttcagta ttactactta caccaaattc ttatatatgc
    97681 tgttctccgc caggattgat tttcgtatta ttggcggccc tacaaaccag ctggatatct
    97741 ttttccagga caacaccatc aactgtctca ccatactcaa cagttgatgc caatgctatg
    97801 ggcgaatata acgctgtagc caccagaaca gatattgcgg aacgacaaag agaaacagaa
    97861 ccagattggt gcattaagct ttcatcctat gaaattaatt gctgttaaaa gcattgggga
    97921 cagaaaatac ccatagctcc atacccggag tcagttttta aaaactgttt aaagaaatgc
    97981 acaagtattg tgattgattt tttagttgtt tttcctgatg agaagctgat gcaaaattcc
    98041 gtctttataa taaagatgat gccaaagcga acgacaaagt tgtagttttc actacatgtc
    98101 catacataaa atggggtaac attcacgcgc ctggtagcgt taccaacgct acgctcaaac
    98161 ataatgattc taataaaacc tcaggagact actatgcctg aagcaacgcc ttttcaggtg
    98221 atgattgtgg atgatcatcc acttatgcga cgcggtgttc gtcagttact ggagcttgat
    98281 tctggctttg aagtggtcgc cgaagcgggc gacggcgcaa gcgcgatcga tctggcgaat
    98341 agactggata tcgacgtgat cttgctggat ctcaatatga aaggcatgag tggcctggat
    98401 accctcaatg ccttgcgcag ggatggcgtt accgcgcaaa ttattatcct gaccgtatca
    98461 gatgcctcca gcgatgtctt tgcactgata gacgcaggcg cagacggtta tctgttgaaa
    98521 gacagcgacc cggaagtgtt actggaagcg attcgtgccg gagcgaaagg cagcaaagtc
    98581 tttagcgaac gcgtcaatca gtacttacgt gaacgtgaaa tgtttggcgc ggaagaagac
    98641 cccttcagcg tgctgacaga acgcgagctg gatgttctgc acgagctggc acaggggctg
    98701 tcaaataaac agattgcctc ggtgttgaat atttccgagc agacagtaaa agtccatatt
    98761 cgcaatctgc tgcgtaaact caacgtccgc tcacgcgtgg cggccaccat tctgttcctg
    98821 caacaacgcg gggcacaata aaaatagccc gatggcatca ccatcgggcc actttttatt
    98881 tactctcctg cggcgacaaa tgttgcatcg cctgcgcgat actacgttcg atcaccgcac
    98941 ggcgagtatc gttggcaggt aagagtttca acatcatttc ccacgcggca acggcttcgc
    99001 caaatcgttg ctgctcaaag gcattaaatg catacatgct taacacacgg atattgctat
    99061 ggtccgttct caccaactgg cgcagcagtt cgccgccgag gcggttgtcg ttgggatcag
    99121 atgaacgtgt caacgcttca gcgtatccca gtgcagcatc gctgtttttc ggatcgaggc
    99181 gatacgcagt ggcgtatgca tcggtggcga tactggcgtt acccagcgcc atgccaacgc
    99241 ggcccaacat aatccagcct tctatatctc ccggattttt ttgcagttga gtacgcatcc
    99301 ccagcgcaag gcgcgacatc tcttcttcgt tgagcggatc ggctttcgga tccagcgccc
    99361 tgtccagtaa cgccggagcc tgtgccgtgg cctgctgcca gattttcacc tgctgataat
    99421 tgccagtctg gtagtagctg acgccagcca caattaacgc caccacaata cccggcagat
    99481 aaacaacata tccggcacgc ttaccttccg gcacgctttg ttctggaaac gcttccggca
    99541 ccacgcgtac ccgacgccgc gaacgggcgt atatgaccca accgccaatg ccaatagcca
    99601 ctactggcag cacccacagc agcacggtca gcggcgttaa cggcggatcg taagtgacga
    99661 agttgccgta acgcgccacc atataatcga caatctcttt cttacttttg ccttcctgca
    99721 tcagttcata cactttctgg cgcaggtcgg tggcaatcat cgagttggaa tcggcaatgc
    99781 tgttgttctg gcatttcggg cagcgcagtt cttcagtgag ctgacggaac tgttgttcct
    99841 gtgcttcatc tttaaactgc aacacgtcga tggtcgccag cgctgagccg gagatcatca
    99901 gcatcagcac gcccaataaa aacctcattg tgcggcctcc ttactgtatt tctcccacag
    99961 tggcttgatc tcttcttccc agacgcgagg attgagatcg cccgcatggc gatagcgaat
   100021 gatgccgttg ccgtcaataa ggaacgtttc tggcgcgcca tagacaccga gatccagccc
   100081 taacatgccg tcgccatcaa acaggcttag cgcgtaagga ttgcccagct ctttcagcca
   100141 gctgattgcc ttctggcgat cgtctttata gttcatgccg accacgcgga tgccctgcgc
   100201 agaaagctga ttcagatatt gatgttccgc acggcaggtc ggacaccagg tcgcccagac
   100261 gttaagcagc actggtttgc cctgagtcag cacatccgcc tgataaaact gccccggatt
   100321 gtccagtgat tcgaggcgaa acttcggcac tggcttgcca atgagcgccg attccagatt
   100381 ggtcggatca tccccttcgg cattacgcgc cagctgccac agcagcgccg cggcaatcgc
   100441 caggaagata atcaacggaa ttaacaatac cttgcgcttc atgcagcctc cggcgcagtt
   100501 ttctgcgggt tcacgcgctt acgatagcga ggatcaaaca gacacagcag tccgcccaac
   100561 gccatcatca gcccgcccgc ccaaatccag cgaacaaatg gtttgtagta aagacgcacg
   100621 gcccacgcgc cgttttccag ctcttcaccg agcgccgcgt acaggtcacg cgtgatgcca
   100681 ccgtcaatcg ccgcttcggt catcatcgac ccggcagtgt tgtaataacg tttttctgca
   100741 tacagcactg tttccggctt gccatctcgc gttacgccga tagtcgccac accgccacgc
   100801 cagtttggac cagtcacttc tttgacatca cggaaggtga agcgatattc atgaatatcg
   100861 acgctatcgc cggacttcat gcgcacatca cgctcaacgc tatagttctg actaaaagca
   100921 atgccaacaa ttgtcactgc cagccccagg tgagctgcca ccatccccca ataactgaag
   100981 gtggttttcg tgccgcgtga aatacgcagc gcagcttccg caattgccag caccgcaatc
   101041 cagcaggcca ttgccaggcc gagcaccgtc atcgccacaa ctttgctttc gaacagccac
   101101 ggcaacagca gcgacagcac cagcgtagag atgaaggcga taatcaacaa attgcggatt
   101161 ttacgtgggc gatcgcgtcc ccagcgcacc agagggccga caccaagcag tagcgcaaac
   101221 ggcaccatca gccaggtaaa catggtgttg aagaacggtt cgccaatcga aatactgccc
   101281 agtcccagtt gcttgtgcac cagcggcagc aacgtcccca gcaacaccac cagcatcgcg
   101341 gctaccagca aaacgttgtt cgctaacagc aaagattccc gcgaccacag cgcattgttt
   101401 acgcgtgagc gaactttgtg tccacgcgcg gcaaacagca gcagcgaacc gccaatcacc
   101461 agcaccataa aggcgaggat aaacataccg cgcgccggat cagacgcaaa cgcgtgtacc
   101521 gataccagca cgccggaacg cacgaggaaa gtccccagca gacacaacga gaaggcactg
   101581 attgccagca gtaatgtcca cgctttgaag ctggcgcgtt gttcagtgac cgccagtgag
   101641 tgcatcagcg cagtccccac cagccacggc ataaacgagg cgttttctac cggatcccag
   101701 aaccaccagc cgccccagcc gagttcgtaa taggcccatg cggaaccgag cacaatgccg
   101761 agcgtcagga agatccacgc agccagcgtc cacggacgag taaaacgcgc ataagtgctg
   101821 tccagacgcc cactcaacaa agatgcaatg gcaaaagcaa acgccaccga gaaaccgacg
   101881 taccccatat agagcagagg cgggtggaaa atcagccccg gatcctgcaa caacgggtta
   101941 agatcgcgcc cttcaatcgg gaaattcggc agcgtgcgtg agaacgggtt agaggtaaag
   102001 agaatgaaca gcaaaaagcc gacactgacc atccccatta tcgccagtac gcgggcaaca
   102061 atatccagcg gaatacgctg actaaaaatc gctaccgcaa aggtccagcc gctcatcagt
   102121 agcacccaca gcaggagcga gccttcatgc gcgccccagg tagccgccac gcgataccac
   102181 accggaagct gggtattgga gttgctggca acataggtga cggtaaagtc gttgaccacg
   102241 aaagcattga ccagcaccag aaatgcgcca gccacagaca taaacagcag ccaggcaaac
   102301 aagcgggaag acgccatcat gcgcgcgtcc ccgcgcgcca cgccccatag cggatacaca
   102361 gacagcagca gcgcaattcc tagcgccaga cacagcagcc cgttaccaat ttctggcatc
   102421 atgatgctgg gtccttataa acactcgccg ggcgacggtg gttagcttcc atcgctttct
   102481 caacttctgg cggcgtgtag ttttcatcgt gtttcgccag cacttctttc gcgaggatat
   102541 gattgccttt ttccagttcg ccctgcacca caacgccctg cccttcacgg aacagatccg
   102601 gcaaaatgcc ttcgtaagag acatctactg aaccttcagc gtcgtaaatg gtgaaagtta
   102661 cttttagcga attgggatcg cgctgcacac tccccggcat caccatcccg ccaacgcgca
   102721 gacgctgacc gacttccggc atctgctgag tttcacgctt gccgtagaga atctcccccg
   102781 gcgtataaaa gagatcgata ttcgagcgca gcgcatatag caccagaccg atagtcagcg
   102841 ccagccccgc cagcacggca caggcaatcc acaagcggtt tttacggcga atattcatgc
   102901 agcctcctgc tgttgcgcag cacgtaaacg cgcctcacgc gcccgctgtt gcgccacgcc
   102961 acgcagaatt gcgcgatgtt gcatcaccga gtgcacgact aaaaccacca gcggaataac
   103021 ggtcatcacc accgccagcc agacaaaaaa ggcgtaaccg cccattgcga aaaattcatt
   103081 ccaggaagca aatgcagggg tcatttacgg cctcttttca gtatcagttc actcacccac
   103141 ggacggcgtt tttccatcag caaaatcaaa ttacgcatcc gcatcagcgt cagcgtggca
   103201 gacaggagca ggaagcccaa aatcgaccag cgcagcggcg aacgcatcgc cggatcgata
   103261 ctttgctgca ttcgcgttga tccctgatgc agggtgttcc accactctac ggagtaatga
   103321 ataatcggca gattcaccac gccaatcagc accaggatac ctgccgcacg cccagccaga
   103381 cgacggtcgt cgaaggcgtg ccacagggca atgacaccaa catacagaaa cagcagcacc
   103441 agttcagaag tcagacgtgc atcccatacc caccaggtgc cccacatcgg ttttccccat
   103501 gcagagccgg taaccagggc aataaaggta aacacggcac caatgggggc catcgccgcc
   103561 accgccaggt tggccatttt catctgccag acaaggccaa taaacgccgc caccgccatt
   103621 gatgcataaa tgcccatcga ccagatcgcc gcaggcacat gcaggtagat aatgcggtag
   103681 ctatttccct gctgataatc agccggagca aagccgaacc cccagatcca gccgacggta
   103741 agcacgacca cactggcaat tgccagccac ggtataaacc agccacagat ttgatacagc
   103801 cgtggtggga tcgccagttg atgcagtgtt ttccacatag tttcgatacc agactcgaac
   103861 aaaaatcagt aatccagcgt tattgaatgc tgattcgtaa cgctgccgcc gtcgcaaaag
   103921 gacttaatgt cgcggtgcct gccagcaacg cgcctaaaat tgccagatac ccatcaacgg
   103981 gcaaatgcat agaagccgcg tccatcgcgg cggtggcaaa gatgagtaat gggatagtca
   104041 gcggtaacac cagtatgctg agcagcacac caccgcgctt aagtcccact gtcagcgcca
   104101 cgcccggcgc accaagaaag ccaagcgtag gggttcccag cagtagcgtt aacgccatca
   104161 cttgccagcc ataaacatcc attcccagta gcattgctac caggggcgaa aggatgagta
   104221 acggcagacc ggttaccatc cagtgcgcca tcaccttcgc cagcacaacg gcgggcaagg
   104281 gtaacggcaa caacatcaat tgttcaagac tgccgtcctg caaatcgtca cggaacagtc
   104341 gctcgagtgc cagcaaggat gaaagcagcg cggcaaccca gataataccc ggcgcaatac
   104401 gcgccagcag ttgcggctcc ggaccgatac tgagcggaaa aagggtaatg acaatcagga
   104461 agaaccacag cgggttggcg atttcggcgc tgtggcgaaa cgctacgcgc agctcaagac
   104521 ggaaaatgcg ccagaacatc atgcggccct cgtttgcgtc agtgaaatgc ggcgaatttt
   104581 actttcagca acgttgagcg gctggtgggt agtcagaatc acaatccccc cctgctccgt
   104641 atgctgcgcc atacgctggg tcagacgatc gacgccatta acgtcaatcg cggtaaaagg
   104701 ctcgtcgagg atccataacg tggcacgggt cagccacaga cgcgctaaag cgacgcggcg
   104761 ttgttgcccg gcagaaagtt gatttaccgg aatatcttcg aatccggcaa gcccggcctg
   104821 cgccagggct tccagacatt gtgcggtatc gccatcgcga tgataaaagt gcagattttc
   104881 taacgccgtc agccgggttt tgatccccgg ctgatggcct atccataaca ggttttgatg
   104941 gtagctgtcg cgtacctgat gcaagggctg cccttgccag agaacctcgc ctgcatcagg
   105001 gcgagacaac cccgtcagca aacggagaag cgttgtcttc cccgcgccgt tgctaccggt
   105061 gatttgtacc cactctcctg cgttcagcgt aaatgataaa ccactaaata aggttcgttc
   105121 atcccgctcg caaagtaact ctctggcttc aagcatactc acgcaataac cctgttaaaa
   105181 acctggctcg acttcacgca tgtcgggcag cttgtgcgct atccctttat ggcaatcaat
   105241 acaggtttgc ccatctttca ccgcctggtc atgcatcttc gcggcaaccg atttctgggc
   105301 ggttgtatcc atatactcga agttgtgaca gttacggcac tcctgcgagt tgttgtcctt
   105361 catgcgccgc cactcattct gtgccatcgt caggcgatga gcttcaaatt tctgcggcgt
   105421 gtcaataacg ccaaaaattt taccatacag ctctttactt gctttgagct tgcgtatcat
   105481 ctttggcaca aactcgtgcg gaacgtgaca atccggacag gtcgcacgga cgccgctacg
   105541 gttgttgtag tgcacggaat ccatgtattc ctgatacacc gtgttacgca tttcgtggca
   105601 gctaatgcag aactcttcgg tattggcttt ttccatcccg gtgttaaagc cgccccagaa
   105661 gacgatgcca ccgacaaaac cgatcaacag cagcgtcccc agcgccagac ggctgggggt
   105721 acgccaccat ttccacaggc gcttaatcag accaggctta cggtcagaat ttcccataat
   105781 aacctcttat ttcccgtaac cttttgacgg ggtaaaggta ttccccacga ttggcgcggt
   105841 atcggcctgc ggtacgtgac attgcagaca gaaataacga cgcggagcca cttccgcgcc
   105901 cactttgccg tcgctgtcca taaagtgagt aggactaatg cgcggcgcgc cagtggtgcg
   105961 ataactttcg acaccgtggc actgcaagca gcggttggta ttggtcgtta cctgataacc
   106021 ttcaacgcta tgcgggatca ttggcggctg attcacatag ttcagcggca tccgatcctg
   106081 ctcttttggc atccgaatgg ccccttcctg cgtcccggat acttccggcg actggctaaa
   106141 atcgacgcca ttagccgccc aaaccgcgcc gcttaccacc agggccagca tcgccgtcca
   106201 ttgacacagc gctttcttca ggtcatggct tttcatgatt tcgctcccga actccatcgt
   106261 gtagttattg taaatacatc ctcagaacag acatccacgc agcgaccgca cgtcatgcaa
   106321 tcgcggctgg tgacctgcac cgggctttgt tcatccagca ccggggcacg tagcacatgc
   106381 ggttccgggc aaacatgaaa acaatccata cagcggttac atttctgccg atcggtggcc
   106441 gcaacggtaa tcacaccttt gctacccagc acgccataca gtgcaccaac cggacaaatg
   106501 tgcccgcacc agccgtgttc aacgaccagt agatcaaata aaaacagcgc gagaataagc
   106561 agcgcgccgc tgccgaagcc cattaccagg ctacgcccca tcagggaaac ggggttgatc
   106621 cattcccaga tgagcgtgcc agttaaggcc gatcccacca gaatgatcac caacagcacg
   106681 tagcgaatgt ggcgagggat cgtcgcagac tgattgaggt caaatcgtct gcgtaaccaa
   106741 tttgctaagt cggtaatcgg gttcagcggg cagacccagc tgcaaaataa tcgctttcct
   106801 gccagggcgt agagcacggt gataatcacc gcaccagtca gcgctaccgt ggcaggcaga
   106861 tgaccgctgg caagactttg cagtgtcatc agcggatcgg tcagtggaac ggtgtcgaat
   106921 aacaggctac tgctgtagtt gccgtgcaag atccacacac caaaccacgg accgctcaaa
   106981 aacatcccca gcacgaagaa ctgacaaagg cgacgtaaca ctaaccaacg gtgactgcgc
   107041 caccagcctt ttttctccag cgcctcgcgc ccggcgtcac gtttacgatt tgccattgtt
   107101 cccctccagc cagccgaagc ggtaatggtg acctaactcc cctttcgcca gtgacagcgg
   107161 taacaccttg attgccggtt gttccagcac gcacactttt tcgcacttac cgcagccggt
   107221 acaggcgtcg ctgtgaaccg tcggcagaaa gcgggcgtgc ttaccggttc gcgtgttgcg
   107281 ctccagctcc agggtgatgg cctcatcaat tttcgggcat tcgcgataac aaacatcgca
   107341 gcgcagcccc tgaaagttga gacagttttc ctggtccacc agtaccgcca gccccatccg
   107401 cgcgtcgtcg atcgattcga tctcacgatc cagcgcaccg ctcgggcaca ctttggcgca
   107461 cggaatgtcc tcacacattt cgcaaggaat atcccgtgcg acaaaatatg gcgtgcccgc
   107521 cgacagacca gaggccagcg tcgccagttt taaggtgtcg taagggcaag cctgaacaca
   107581 ctgaccgcaa cgcacacagg cactggcaaa ggcgttctcg tttatggctc cgggcgggcg
   107641 caaccgcaca ccagatgcgc gtgcggtttg ctgttgtaac cccagcgcca cacccacggc
   107701 agccagcccg cctgctgtgc gaacgacatc gcgcagaaag cggcggcgac cattttgagg
   107761 tttcgctgac cgggacataa tggcttacac cttctccagt ttgaccgcgc acttcttgaa
   107821 gtccgtctct ttcgagagcg gatcggtcgc atccagcgtc agtttgttaa ccagctgtgc
   107881 ggcgtcgaag aacggcatgt acaccaggcc ctgcggcgga cggttacgac cgcgcgtttc
   107941 gacaatcgag atcacttcgc cacggcgaga aaccactttc actttgtcgc cacggcgcag
   108001 atcgcgcgct ttcgcatcca gcgggtgaat aaacagaacc gcttccggga aggcgcggtg
   108061 cagttccggt acacggcgag tcatactgcc ggtatgccag tgctccagga cgcgtccggt
   108121 agagagccac aggtcgtact cttcatccgg cgcttccgcc gcaggttcga acggcagcgc
   108181 gaagatcacc gctttgccat ccggtttacc gtagaacttg tagccttcgc ccgctttcac
   108241 gtacgggtcg ttaccttcgc tgtaacgcca ctgcgtttct ttaccgttca ccaccggcca
   108301 gcgcagaccg cgtgctttgt ggtaatcatc gaacggcgcg agatcgtgac cgtgaccgcg
   108361 accaaaccat gcgtactctt cgaacaaccc tttttgcaga tagaagccca gctcgcgaga
   108421 ttcgtcgttc agctgatctt ccgccagttc ggataccggg aatttgctca cttccggagt
   108481 ggcatacaga acctcataca gcgttttgcc acgcagttcc ggtttcttag ccagcagctc
   108541 ttccggccac acttcttcag ttttgaagcg acgggagaac tgaactaact gccagagatc
   108601 cgattttgct tcacccggtg cctgtacctg ctgacgccag aactgagtac ggcgttcggc
   108661 attaccgtaa gccccctctt tctctaccca cattgcggtc ggcaggatca agtccgctgc
   108721 cagcgcactg accgtcggat atggatcgga aacgatgata aagttgcgcg gatcgcgcca
   108781 gcccggcata cgctcttcat taatgttcgg tccggcctgc atgttattgg tacacatggt
   108841 ccagtaaaca ttcagtttgc cgtctttcag cgcacggtct tgtgccaccg cgtgcagacc
   108901 gattttcgcc ggaatggtgc cactcgggat attccacttc ttctcgcaaa tatcacgatg
   108961 tttctcgtta gtcaccacca tgtccgcagg cagacggtga gcaaaggtgc ccacttcacg
   109021 cgcagtacca cacgcggaag gctgcccggt cagggagaac ggaccgcaac ccggctggga
   109081 aattttgccg gtcagcaggt gcaggttgta gaccaggttg ttggcccaca caccacgggt
   109141 atgctggttg aagcccatcg tccagtagga gatgactttc ttgttcggat cggcatacag
   109201 ctgcgccagt tgttctaact ggtctttcgg cacgccggtc atttcggcgg ttttttccag
   109261 cgtatattca gcaacgaagg ctttgtaatc ttcaaagctc atcggttcgg aggcgtcaga
   109321 acccggattc ttcgctgctt tttccagcgg atgggtcgga cgtaaaccgt agccgatgtc
   109381 cgtcgcccct ttgcgcaggt taacgtgttt gctgaagaag tcctgattta tcgcattgtt
   109441 ttgaatgata tagttggcga tatagttcag gatcaccagg tcagattgcg gcgtaaagat
   109501 gatgccgtta tccgccagct cgaagctacg atgctggtag gtagaaagca ccgccacggt
   109561 aacgttctgg ttagagagac gacggttagt gatgcgcgac cagaggatcg ggtgcatctc
   109621 cgccatgttt gcgccccaca gcacaaacgc gtcagcctgc tcgatgtcgt catagcagcc
   109681 catcggctca tccataccaa aggtacgcat aaagccaact actgccgacg ccatacagtg
   109741 acgcgcgttc gggtcgatgt tgttcgaacg gaagcccgct ttaaacagct tggacgcggc
   109801 ataaccttcc cagatagtcc actgaccaga accgaacata ccgatcgatt ccggcccttt
   109861 ttctttcagg gcggttttga atttctcttc catcacatcg aaggcctgat cccaggtgat
   109921 tggggtaaat tcgccttctt tatcatattt accgtttttc atacgcagca gcggctgcgt
   109981 caaacggtct ttaccgtaca tgattttggg caggaaatag cccttaatgc agttcaggcc
   110041 acggttaacc ggtgcgtccg ggtcgccctg acaagccacc acacgcccct gctgcgttcc
   110101 gaccagaacg ccgcaacccg taccgcagaa acggcacggc gctttatccc atttaatggc
   110161 ttcctgctga ccaacaacgg cgcgggcaac gcccggcacg ctgagaccgg cagccgccgc
   110221 agcggccgca acggcgttag ctttcataaa gctacgacga ctgagtttca tggtgtttcc
   110281 tcaccttgct cttcctgctg gtgataaacc agcgacaccg ccagcacgcc ctctacgttg
   110341 cgtactgact caatggtttg gatcagcgtt tcgctgtctt ctgcttccac caccacaatc
   110401 aactgaccgc tcggcgcgtc gctgacagca acttcacagc cggtaaaggc gttcagttgg
   110461 gtgctgatgt ctgaaattcg ttcgcttttg gcctgcacga ccaggctgca aacttgccag
   110521 ttagtgtgca tggagatact ccgcagttat ggctgatacc ggacagctgg ctgcacatgc
   110581 tccacatccg ttgcaaagtt ggctattaag ttgcggctgg tagatcccgg agagcgtcgg
   110641 gcgaaagata attgccattg gctcacagct atcctgacag cggcggcatt caactgactg
   110701 atacgccagg cacgcgtccc caatggtgaa ctgcaaatcc caggccctgg tgtggcgcgg
   110761 agaaaatagc gattcggggc aagcctgcgc gcaggcgtaa cagaagctgc actcgttatt
   110821 tttgaaatta acgctcggat agctacccgc gccgcgttgc agaatgttgt tttcacaagc
   110881 attaatacag gcgtcgcagc gggtgcaatg ggtcagaaaa tgagattcat caccggacca
   110941 gggcggacgg ataccgttac tggctttgcg ccagcgacca gtgagtatgc cccgacggga
   111001 tgcatcaatc ttcacattga ccttccatca ttaacgcgct ctgaaaattg agagcgacca
   111061 aataaaccgc ataattaata agccattttt atagccgcta agatattaaa ggatgtgtca
   111121 aagatgcata ccccgatcgg ggtaaaacct gtagcaggat caaaagagtg gcgggaagcg
   111181 tggcaaaaac gggcttttgc tcacatttca aatggttata aacatattta tatagcgatt
   111241 aattcgccag agatatttct gctggtttgt tttc
//
LOCUS       DACADF010000002        87144 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.002, whole genome shotgun sequence.
ACCESSION   DACADF010000002 DACADF010000000
VERSION     DACADF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.002"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(183..1583)
                     /gene="asnS"
                     /locus_tag="ISB30_000097"
     CDS             complement(183..1583)
                     /gene="asnS"
                     /locus_tag="ISB30_000097"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP1987784.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPNTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1752..2954)
                     /gene="pncB"
                     /locus_tag="ISB30_000098"
     CDS             complement(1752..2954)
                     /gene="pncB"
                     /locus_tag="ISB30_000098"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP1987785.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPLEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3220..5832
                     /gene="pepN"
                     /locus_tag="ISB30_000099"
     CDS             3220..5832
                     /gene="pepN"
                     /locus_tag="ISB30_000099"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAP1987786.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(5875..6642)
                     /gene="ssuB"
                     /locus_tag="ISB30_000100"
     CDS             complement(5875..6642)
                     /gene="ssuB"
                     /locus_tag="ISB30_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAP1987787.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGEFETRLRKQG"
     gene            complement(6639..7430)
                     /gene="ssuC"
                     /locus_tag="ISB30_000101"
     CDS             complement(6639..7430)
                     /gene="ssuC"
                     /locus_tag="ISB30_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HAP1987788.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRELVEMARS
                     YGLSGMPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7442..8587)
                     /gene="ssuD"
                     /locus_tag="ISB30_000102"
     CDS             complement(7442..8587)
                     /gene="ssuD"
                     /locus_tag="ISB30_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAP1987789.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPKLVKEKIEHVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQASFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(8584..9543)
                     /locus_tag="ISB30_000103"
     CDS             complement(8584..9543)
                     /locus_tag="ISB30_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1987790.1"
                     /translation="MRKIIKLVLAGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9536..10111)
                     /gene="ssuE"
                     /locus_tag="ISB30_000104"
     CDS             complement(9536..10111)
                     /gene="ssuE"
                     /locus_tag="ISB30_000104"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAP1987791.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            10348..11358
                     /gene="pyrD"
                     /locus_tag="ISB30_000105"
     CDS             10348..11358
                     /gene="pyrD"
                     /locus_tag="ISB30_000105"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAP1987792.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            11532..12074
                     /gene="zapC"
                     /locus_tag="ISB30_000106"
     CDS             11532..12074
                     /gene="zapC"
                     /locus_tag="ISB30_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAP1987793.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(12071..13180)
                     /gene="ycbX"
                     /locus_tag="ISB30_000107"
     CDS             complement(12071..13180)
                     /gene="ycbX"
                     /locus_tag="ISB30_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAP1987794.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRALQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPADEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADGTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSATGDDGTILCCSCVPKTALKLAR"
     gene            13278..15386
                     /gene="rlmKL"
                     /locus_tag="ISB30_000108"
     CDS             13278..15386
                     /gene="rlmKL"
                     /locus_tag="ISB30_000108"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAP1987795.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETANIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            15398..17305
                     /locus_tag="ISB30_000109"
     CDS             15398..17305
                     /locus_tag="ISB30_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1987796.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELELLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            17435..18688
                     /gene="pqiA"
                     /locus_tag="ISB30_000110"
     CDS             17435..18688
                     /gene="pqiA"
                     /locus_tag="ISB30_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAP1987797.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            18693..20333
                     /gene="pqiB"
                     /locus_tag="ISB30_000111"
     CDS             18693..20333
                     /gene="pqiB"
                     /locus_tag="ISB30_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAP1987798.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            20330..20893
                     /gene="pqiC"
                     /locus_tag="ISB30_000112"
     CDS             20330..20893
                     /gene="pqiC"
                     /locus_tag="ISB30_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAP1987799.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLARVWSQEAASIAQEIKRLP"
     gene            21149..21316
                     /gene="rmf"
                     /locus_tag="ISB30_000113"
     CDS             21149..21316
                     /gene="rmf"
                     /locus_tag="ISB30_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAP1987800.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(21386..21904)
                     /gene="fabA"
                     /locus_tag="ISB30_000114"
     CDS             complement(21386..21904)
                     /gene="fabA"
                     /locus_tag="ISB30_000114"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAP1987801.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(21973..23733)
                     /locus_tag="ISB30_000115"
     CDS             complement(21973..23733)
                     /locus_tag="ISB30_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAP1987802.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            23919..24371
                     /gene="matP"
                     /locus_tag="ISB30_000116"
     CDS             23919..24371
                     /gene="matP"
                     /locus_tag="ISB30_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAP1987803.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(24447..25499)
                     /gene="ompA"
                     /locus_tag="ISB30_000117"
     CDS             complement(24447..25499)
                     /gene="ompA"
                     /locus_tag="ISB30_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAP1987804.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(25855..26364)
                     /gene="sulA"
                     /locus_tag="ISB30_000118"
     CDS             complement(25855..26364)
                     /gene="sulA"
                     /locus_tag="ISB30_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAP1987805.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTAEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            26583..27212
                     /gene="sxy"
                     /locus_tag="ISB30_000119"
     CDS             26583..27212
                     /gene="sxy"
                     /locus_tag="ISB30_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAP1987806.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     CLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(27175..29337)
                     /gene="yccS"
                     /locus_tag="ISB30_000120"
     CDS             complement(27175..29337)
                     /gene="yccS"
                     /locus_tag="ISB30_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAP1987807.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(29347..29793)
                     /locus_tag="ISB30_000121"
     CDS             complement(29347..29793)
                     /locus_tag="ISB30_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP1987808.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            29916..31970
                     /gene="helD"
                     /locus_tag="ISB30_000122"
     CDS             29916..31970
                     /gene="helD"
                     /locus_tag="ISB30_000122"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAP1987809.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGMQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(32002..32460)
                     /gene="mgsA"
                     /locus_tag="ISB30_000123"
     CDS             complement(32002..32460)
                     /gene="mgsA"
                     /locus_tag="ISB30_000123"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP1987810.1"
                     /translation="MELTTRTLPSRKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(32556..33218)
                     /locus_tag="ISB30_000124"
     CDS             complement(32556..33218)
                     /locus_tag="ISB30_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAP1987811.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            33391..33804
                     /locus_tag="ISB30_000125"
     CDS             33391..33804
                     /locus_tag="ISB30_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP1987812.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(33849..34166)
                     /gene="hspQ"
                     /locus_tag="ISB30_000126"
     CDS             complement(33849..34166)
                     /gene="hspQ"
                     /locus_tag="ISB30_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAP1987813.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(34224..35414)
                     /gene="rlmI"
                     /locus_tag="ISB30_000127"
     CDS             complement(34224..35414)
                     /gene="rlmI"
                     /locus_tag="ISB30_000127"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAP1987814.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGV
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            35509..35787
                     /gene="yccX"
                     /locus_tag="ISB30_000128"
     CDS             35509..35787
                     /gene="yccX"
                     /locus_tag="ISB30_000128"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP1987815.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(35784..36113)
                     /gene="tusE"
                     /locus_tag="ISB30_000129"
     CDS             complement(35784..36113)
                     /gene="tusE"
                     /locus_tag="ISB30_000129"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAP1987816.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(36205..36864)
                     /gene="yccA"
                     /locus_tag="ISB30_000130"
     CDS             complement(36205..36864)
                     /gene="yccA"
                     /locus_tag="ISB30_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAP1987817.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(37073..37160)
                     /locus_tag="ISB30_000131"
     tRNA            complement(37073..37160)
                     /locus_tag="ISB30_000131"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37124..37126),aa:Ser,seq:tga)
     gene            37588..38706
                     /gene="hyaA"
                     /locus_tag="ISB30_000132"
     CDS             37588..38706
                     /gene="hyaA"
                     /locus_tag="ISB30_000132"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAP1987818.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            38703..40496
                     /gene="hyaB"
                     /locus_tag="ISB30_000133"
     CDS             38703..40496
                     /gene="hyaB"
                     /locus_tag="ISB30_000133"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAP1987819.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQLIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            40515..41222
                     /gene="hyaC"
                     /locus_tag="ISB30_000134"
     CDS             40515..41222
                     /gene="hyaC"
                     /locus_tag="ISB30_000134"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAP1987820.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            41219..41806
                     /locus_tag="ISB30_000135"
     CDS             41219..41806
                     /locus_tag="ISB30_000135"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAP1987821.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEDVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGIRLRQYRMTLEEQG"
     gene            41803..42201
                     /gene="hyaE"
                     /locus_tag="ISB30_000136"
     CDS             41803..42201
                     /gene="hyaE"
                     /locus_tag="ISB30_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAP1987822.1"
                     /translation="MSNDTPFDALWQRMLARGWTPISESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTCGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            42198..43055
                     /locus_tag="ISB30_000137"
     CDS             42198..43055
                     /locus_tag="ISB30_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAP1987823.1"
                     /translation="MSETFFHLLGPGTQLNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DGSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            43189..44733
                     /gene="appC"
                     /locus_tag="ISB30_000138"
     CDS             43189..44733
                     /gene="appC"
                     /locus_tag="ISB30_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAP1987824.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            44745..45881
                     /gene="appB"
                     /locus_tag="ISB30_000139"
     CDS             44745..45881
                     /gene="appB"
                     /locus_tag="ISB30_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAP1987825.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            45894..45986
                     /gene="cbdX"
                     /locus_tag="ISB30_000140"
     CDS             45894..45986
                     /gene="cbdX"
                     /locus_tag="ISB30_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAP1987826.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            46066..47376
                     /locus_tag="ISB30_000141"
     CDS             46066..47376
                     /locus_tag="ISB30_000141"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAP1987827.1"
                     /translation="MKAILIPFLSLLIPLTPKSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKEGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALAPHPPQKQVYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEVRIPACALHQDK"
     gene            complement(47364..47576)
                     /gene="cspH"
                     /locus_tag="ISB30_000142"
     CDS             complement(47364..47576)
                     /gene="cspH"
                     /locus_tag="ISB30_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HAP1987828.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            47862..48074
                     /gene="cspG"
                     /locus_tag="ISB30_000143"
     CDS             47862..48074
                     /gene="cspG"
                     /locus_tag="ISB30_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HAP1987829.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            48085..48273
                     /locus_tag="ISB30_000144"
     CDS             48085..48273
                     /locus_tag="ISB30_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP1987830.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            48248..48478
                     /gene="ymcE"
                     /locus_tag="ISB30_000145"
     CDS             48248..48478
                     /gene="ymcE"
                     /locus_tag="ISB30_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HAP1987831.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            48468..48641
                     /locus_tag="ISB30_000146"
     CDS             48468..48641
                     /locus_tag="ISB30_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAP1987832.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(48690..49763)
                     /locus_tag="ISB30_000147"
     CDS             complement(48690..49763)
                     /locus_tag="ISB30_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAP1987833.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFVTRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLAFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAMYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(49846..52578)
                     /gene="torS"
                     /locus_tag="ISB30_000148"
     CDS             complement(49846..52578)
                     /gene="torS"
                     /locus_tag="ISB30_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HAP1987834.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGLVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATEIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRIDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRVPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNSSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAHLMGAEKIHEWLILFKQHALPLLDEIDIARASQDNEKIK
                     RAAHQLKSSCSSLGMRSASQQCAQLEQQPLSAPLPHEEITRSVAALEAWLIRKT"
     gene            52661..53689
                     /gene="torT"
                     /locus_tag="ISB30_000149"
     CDS             52661..53689
                     /gene="torT"
                     /locus_tag="ISB30_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAP1987835.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPHVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(53662..54354)
                     /gene="torR"
                     /locus_tag="ISB30_000150"
     CDS             complement(53662..54354)
                     /gene="torR"
                     /locus_tag="ISB30_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAP1987836.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            54484..55656
                     /gene="torC"
                     /locus_tag="ISB30_000151"
     CDS             54484..55656
                     /gene="torC"
                     /locus_tag="ISB30_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAP1987837.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            55656..58202
                     /gene="torA"
                     /locus_tag="ISB30_000152"
     CDS             55656..58202
                     /gene="torA"
                     /locus_tag="ISB30_000152"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HAP1987838.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVSTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            58199..58798
                     /gene="torD"
                     /locus_tag="ISB30_000153"
     CDS             58199..58798
                     /gene="torD"
                     /locus_tag="ISB30_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAP1987839.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNGLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLDLLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LREWLPEFAARCRQYDSFGFYAALSQLLLVLVECDYQKR"
     gene            complement(59053..59358)
                     /gene="cbpM"
                     /locus_tag="ISB30_000154"
     CDS             complement(59053..59358)
                     /gene="cbpM"
                     /locus_tag="ISB30_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAP1987840.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(59358..60278)
                     /gene="cbpA"
                     /locus_tag="ISB30_000155"
     CDS             complement(59358..60278)
                     /gene="cbpA"
                     /locus_tag="ISB30_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAP1987841.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            60813..62054
                     /gene="agp"
                     /locus_tag="ISB30_000156"
     CDS             60813..62054
                     /gene="agp"
                     /locus_tag="ISB30_000156"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAP1987842.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTERTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            complement(62092..62319)
                     /locus_tag="ISB30_000157"
     CDS             complement(62092..62319)
                     /locus_tag="ISB30_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987843.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(62340..62936)
                     /gene="wrbA"
                     /locus_tag="ISB30_000158"
     CDS             complement(62340..62936)
                     /gene="wrbA"
                     /locus_tag="ISB30_000158"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAP1987844.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            63309..63482
                     /locus_tag="ISB30_000159"
     CDS             63309..63482
                     /locus_tag="ISB30_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAP1987845.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(63565..64893)
                     /gene="rutG"
                     /locus_tag="ISB30_000160"
     CDS             complement(63565..64893)
                     /gene="rutG"
                     /locus_tag="ISB30_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAP1987846.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGITTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQKP"
     gene            complement(64914..65408)
                     /gene="rutF"
                     /locus_tag="ISB30_000161"
     CDS             complement(64914..65408)
                     /gene="rutF"
                     /locus_tag="ISB30_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAP1987847.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEKALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(65419..66009)
                     /locus_tag="ISB30_000162"
     CDS             complement(65419..66009)
                     /locus_tag="ISB30_000162"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAP1987848.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWREPPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSDLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(66019..66819)
                     /gene="rutD"
                     /locus_tag="ISB30_000163"
     CDS             complement(66019..66819)
                     /gene="rutD"
                     /locus_tag="ISB30_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HAP1987849.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLDQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINTHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(66827..67213)
                     /gene="rutC"
                     /locus_tag="ISB30_000164"
     CDS             complement(66827..67213)
                     /gene="rutC"
                     /locus_tag="ISB30_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAP1987850.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(67225..67917)
                     /gene="rutB"
                     /locus_tag="ISB30_000165"
     CDS             complement(67225..67917)
                     /gene="rutB"
                     /locus_tag="ISB30_000165"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAP1987851.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(67917..69008)
                     /gene="rutA"
                     /locus_tag="ISB30_000166"
     CDS             complement(67917..69008)
                     /gene="rutA"
                     /locus_tag="ISB30_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAP1987852.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVANVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            69296..69934
                     /gene="rutR"
                     /locus_tag="ISB30_000167"
     CDS             69296..69934
                     /gene="rutR"
                     /locus_tag="ISB30_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAP1987853.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(69974..73936)
                     /gene="putA"
                     /locus_tag="ISB30_000168"
     CDS             complement(69974..73936)
                     /gene="putA"
                     /locus_tag="ISB30_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP1987854.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQSLPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDNALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            73991..74200
                     /locus_tag="ISB30_000169"
     CDS             73991..74200
                     /locus_tag="ISB30_000169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987855.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            74359..75867
                     /gene="putP"
                     /locus_tag="ISB30_000170"
     CDS             74359..75867
                     /gene="putP"
                     /locus_tag="ISB30_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HAP1987856.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNGHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            76087..76917
                     /locus_tag="ISB30_000171"
     CDS             76087..76917
                     /locus_tag="ISB30_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HAP1987857.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGVWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            76975..78102
                     /locus_tag="ISB30_000172"
     CDS             76975..78102
                     /locus_tag="ISB30_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAP1987858.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKEGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            78108..79379
                     /gene="efeB"
                     /locus_tag="ISB30_000173"
     CDS             78108..79379
                     /gene="efeB"
                     /locus_tag="ISB30_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAP1987859.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            79730..80794
                     /gene="phoH"
                     /locus_tag="ISB30_000174"
     CDS             79730..80794
                     /gene="phoH"
                     /locus_tag="ISB30_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAP1987860.1"
                     /translation="MVTSCTGHVLENQRATTRGDFSSGSHLVTLYFQPHPFFSCVTDA
                     VNGARSRFSAFYPKANYGLQGSQPSDVRAHNRAANGACDEYKQLKVLSMGRQKAVIKA
                     RREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPILARNEAQLHYL
                     KAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQADEDLGFLPGD
                     IAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVEIAPFAYMRGRTFENAVVILDE
                     AQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVRSGLSDALERFEEDEMVGIVR
                     FGKEDCVRSALCQRTLHAYS"
     gene            complement(80844..81257)
                     /gene="pgaD"
                     /locus_tag="ISB30_000175"
     CDS             complement(80844..81257)
                     /gene="pgaD"
                     /locus_tag="ISB30_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HAP1987861.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAIDLLTGY
                     FWQSEARSRLQFYFLLAVVNAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKTLVRV"
     gene            complement(81259..82584)
                     /gene="pgaC"
                     /locus_tag="ISB30_000176"
     CDS             complement(81259..82584)
                     /gene="pgaC"
                     /locus_tag="ISB30_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HAP1987862.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIIGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPRLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWNQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAAQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLVIENRYEHNLA
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            complement(82577..84595)
                     /gene="pgaB"
                     /locus_tag="ISB30_000177"
     CDS             complement(82577..84595)
                     /gene="pgaB"
                     /locus_tag="ISB30_000177"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HAP1987863.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVVISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDEMVS
                     REYFATWQQVREVARSRLVEVASHTWDSHYGIQANATGSLLPAYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIAELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKPLQRVMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     DDLAGHAAFDGILFHDDALLSDYEDASAAAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALADFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVTEKSADQWLIQLTNQMKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            complement(84604..87027)
                     /gene="pgaA"
                     /locus_tag="ISB30_000178"
     CDS             complement(84604..87027)
                     /gene="pgaA"
                     /locus_tag="ISB30_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HAP1987864.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFAQKSTLSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALFKLKQLKS
                     GAPDKANLLAEAYIYKLTGRYQDELRAMTESLPENASKQQYPTEYVLALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSENERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYRDVISHYQRLKKTGQIIPPWALYWVASAYLKDQQPKKAQS
                     IMTELFYNKETIATDLSDEELADLFYSHLESENYPGALTVTKHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEITTRELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSIE
                     GQERIWSSPYLIVDFLPNLYYEQNTEHDTPYYNPIKMFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
BASE COUNT        21354 a        21991 c        22970 g        20829 t
ORIGIN      
        1 aaaaactaat ccgcaataag aatgcagatt attttttgtg cgtaaatgtt gcagtgtaat
       61 attccgtaac aggatgatcg ttatggcagg gttgcaaata gattgcttga cttaacaaac
      121 agataacttg acagaaagga taaaaaaaga gccagcgggc gctggctctt gaagacacta
      181 aattagaagc tggcgttacg cggagtacgt gggaacggaa taacatcacg tacgttttgc
      241 acgccagtta cgtaagcaat caggcgttca aaaccaagac cgaaacctga atgcggaaca
      301 gtaccgtagc gacgcagatc gcgataccac cagtaatctt ctttgttcag gcccatttca
      361 agcatacgct cgtccagcac gtccagacgt tcttcacgct gggagccacc aatgatctca
      421 ccgatgcccg gagccagaac gtccatagcc gcaacggttt taccgtcttc gttaaggcgc
      481 atatagaacg ctttaatatc tttcggatag tttttaacca ctactggtgc tttaaagtgt
      541 tcttccgcca gataacgctc atgctcagaa gagagatcga cgccccagta aaccgggttt
      601 tcaaacttcc tgccgcagtt ttcgagaatg gtcactgcgt cggtgtaatc cacctgcgcg
      661 aaatcggcct caatgaagcg ttccagacgt gaaacggcat ctttatctac gcgttcagcg
      721 aagaatttca tgtcgtcagc gcgttcttcg agaaccgctt tgaagacata tttcagcatg
      781 gcttcagcca gacctgcgat atcgttcagg ttagcaaacg ccacttccgg ctccagcatc
      841 cagaattccg ccaggtgacg gctggtgttg gagttttcag cacggaaagt cgggccgaag
      901 gtataaattt tggacaatgc gcaagcgtag gtttcgccgt tcaactggcc agatacggtc
      961 aggaaagact ctttaccaaa gaagtctttg tcgaaatcca ctttgccctg atcgttacgc
     1021 ggcaggtttt ccagatccag cgtagaaaca cggaacattt cgcctgcacc ttcggtatca
     1081 gatgcggtaa tcagtggcgt tgaaacccag aagaatccct gctcgttaaa gaagcgatgc
     1141 agcgcctgcg ccagcgtatg gcgaacgcgc gcgacggcac caatcaggtt tgtgcgcgga
     1201 cgcaggtgag cgacttcacg cagatactca atgctgtggc gttttgccgc catcgggtaa
     1261 gtgtttggat cttcaaccca accagcaact tcaaccttgc tggcctgaat ttcaaattgt
     1321 tgcccctggc ccggcgacgc cacgacttta cccgttacaa tgaccgagca gccggtggtc
     1381 agacgcagga cgtcttcatt gtaattgggc agagaattat tgatgacagc ctgtacagga
     1441 tcaaagcagg aaccgtcata aacggcgagg aaggagatgc cagcttttga atctcggcgg
     1501 gtacgtaccc atccgcgcac ggtgacttcg ctgtcaacgg ctacgcggcc ctggagtacg
     1561 tcggctacag gcacaacgct cataatattc tctctgttaa tagtcggaaa aaataaacac
     1621 ttgtccaccc gtaaatgggg ggatacctat gttacctggc atctgcaatc agacaagcag
     1681 aattcgcaaa tgcagcgaaa gatttcggaa ttaaaggtaa aaaaagggag ccgattagct
     1741 ccctgatgat attaactggc ttttttaata tgcggaaggt cgaacgcttt gcgcagcgcc
     1801 cgaacaaacg ctttatcgtg gcagatcgtt ttgccagggc tgtcagaaag tttcgccacc
     1861 ggtttaccgt tacactctac caacttaatg acaatattca gaggttttac ctgggggata
     1921 tcgcaggtca ggcgagtccc aataccaaaa cttaattgca cgcgggaaga gaagtggcgg
     1981 tatagctcaa ccgctttgcg taaatccaga ttgtcagaga aaaccagcgt tttactctgt
     2041 ggatcaattc ccagcttttc ataatgtgca atggcttttt caccccattc aagcgggtcg
     2101 ccagagtcat ggcgcaggcc ctggtaacga ctggcaaatt ccacgccgaa atctcgcagg
     2161 aaagcatcca tggtgatgca gtcggttaac gcaatgccaa gttggtcggg atactcttcc
     2221 agccaggcag caagtgcagc tcgctggctg ttggctagat ccgggctgat ttgctgatgc
     2281 gcctggaacc attcgtgtgc ctgtgttccc attggcgtga gggaaagccg acgcgccaga
     2341 tcgtagttgc tggtgcccac gaaccaggat tcctgttgca gacgtttcac gatggtttct
     2401 tgtacttcgc gagaaaaacg gcggcgggtg ccaaaatcca tcagatggaa gcgcgacata
     2461 tcaagaccgg cggttaatgc cgagaagtcg actaatttgt tttccagcgt gtcgagggct
     2521 tgcgcaacgt cggcctgcgg tgagcgatag cgatgtacca tttcactgat aaccgccagc
     2581 aaaggaactt cccagaggat gacttcacgc cacgggccgc ttaaacgaat atccagcttg
     2641 ccattatcgt tggacacggt gacttgttcc gggttaaagc ggaactcgcg tagccagtta
     2701 agatagtcgg ccttaaagaa aggcagggca gagagccact gatattcatc atcctgcagg
     2761 cgcaggtgct gcatcgcctg aacctgttca cgaatagcat cggcataaat acccagcaga
     2821 tcgtcacctc ggcaacgaaa ctccgccgcg acatgcacat cgtaatagtg atgaaacacg
     2881 gcttgctgca tatgcaactt ataagcatct gtatccagca acgagtgcag aacaggagaa
     2941 gcgaattgtg tcataggtgc gcagtagcgt cctcgtacag gagcgtttag tacaataaac
     3001 atcttcagga actgctggag tataccttgt ttcgcaattt attgaacccc gatcacacca
     3061 tatgccacct ttctggtcga tggcataacg cggtgtatgt tataaaaatg tagcaataaa
     3121 ggcgtttgta cctgaaaaga tgaagattct gcatagcgcg atttacgcaa caggaataga
     3181 ctgaacacca gactctataa aagatgctaa aggttattta tgactcaaca gccacaagcc
     3241 aaataccgtc acgattatcg tgcgccggat taccagatta ctgatattga cttgaccttt
     3301 gacctggacg cgcaaaagac ggtcgttacc gcggtcagcc aggctgtccg tcatggtgca
     3361 tcagatgttc cgcttcgtct caacggcgaa gacctcaaac tggtttctgt tcatattaat
     3421 gatgagccgt ggaccgcctg gaaagaagaa gagggcgcac tggtcatcag taatttgccg
     3481 gagcgtttta cgcttaagat cgttaatgaa attagcccgg cggcgaatac tgcgctggaa
     3541 gggctttatc agtcaggcga tgcgctttgc acccagtgtg aagccgaagg tttccgccat
     3601 attacgtatt atctcgaccg cccggacgtg ctggcgcgtt ttaccaccaa aattattgcc
     3661 gataaaacca aatatccctt cctgctttcc aacggtaacc gcgttgcgca aggtgaactg
     3721 gaaaatggac gtcattgggt acagtggcag gatccattcc cgaaaccgtg ttacctgttt
     3781 gcgctggtgg ctggtgattt tgatgtactg cgcgatacct tcactacccg ctctggtcgt
     3841 gaagtggcgc tggagttgta tgtcgatcgt ggcaaccttg atcgtgcgcc gtgggcaatg
     3901 acctcgctga aaaactccat gaaatgggat gaagaacgct tcggcctgga gtatgacctc
     3961 gacatctata tgatcgtcgc ggtggatttc ttcaatatgg gcgcaatgga gaataagggg
     4021 ctgaatatct ttaactccaa atatgtgctg gcccgcaccg acaccgccac cgacaaagat
     4081 tacctcgata ttgaacgcgt tatcggccat gaatatttcc ataactggac cggcaaccgt
     4141 gtcacctgcc gcgactggtt ccagctcagt ctgaaagaag gtttaaccgt cttccgcgat
     4201 caggaattca gctctgacct tggttcccgc gcggtaaacc gtatcaataa tgtgcgcacc
     4261 atgcgcggat tgcagtttgc agaagacgcc agcccgatgg cgcacccaat tcgcccggat
     4321 atggtcattg aaatgaacaa cttctacacc ctgaccgttt acgagaaggg cgcggaagtg
     4381 attcgtatga tccacactct gcttggcgaa gaaaatttcc agaaagggat gcagctctat
     4441 ttcgagcgtc atgatggtag cgcagcgacc tgtgacgatt ttgtgcaggc gatggaagat
     4501 gcgtcgaatg tcgatctctc ccatttccgc cgttggtaca gccagtccgg cacgccggtt
     4561 gtgaccgtca aagacgacta caatccggaa actgagcagt acaccctgac catcagccag
     4621 cgcacgccag ctacgccgga tcaggcagaa aaacaaccgc tgcatattcc gtttgccatc
     4681 gaactgtatg acaacgaagg caaagtgatc ccgttgcaga aaggcggtca tccggtgaat
     4741 tccgtgctga acgtcaccca ggcggaacag acctttgttt ttgataatgt ctacttccag
     4801 ccggtgcctg cgctgctgtg cgaattctct gcgccagtga aactggaata taagtggagc
     4861 gatcagcaac tgaccttcct gatgcgtcat gcgcgtaatg atttctctcg ctgggacgcg
     4921 gcgcaaagtt tgctggcaac ctacatcaag ctgaacgtcg cccgtcatca gcaagggcag
     4981 ccgttgtctc tgccggtgca tgtggctgac gctttccgcg cggtgctgct cgatgagaag
     5041 attgatccgg cgctggcggc agaaatcctg acgctgcctt ccgtcaatga aatggcagaa
     5101 ctgttcgata tcatcgatcc gattgctatt gccgaagtac gcgaagcact cactcgtact
     5161 ctggcgactg aactggcaga tgagctgctg gctatttaca acgcgaatta ccagagcgag
     5221 taccgtgttg agcatgaaga tattgcaaaa cgcactctgc gtaatgcctg cctgcgcttc
     5281 ctcgcttttg gtgaaacgca tctggctgat gtgctggtga gcaaacagtt ccacgaagcg
     5341 aacaatatga ccgatgcgct ggcggcgctt tctgcggcgg ttgctgcaca actgccatgc
     5401 cgtgacgcgc tgatgcagga gtacgacgac aagtggcatc aggatggtct ggtgatggat
     5461 aaatggttta tcctgcaagc caccagcccg gcggcgaatg tgctggagac ggtacgtggt
     5521 ctgttgcagc atcgctcatt taccatgagc aacccgaacc gcattcgttc attgattggc
     5581 gcatttgcgg gcagcaaccc ggcagcgttc catgccgaag atggcagcgg ttatcagttc
     5641 ctggtggaaa tgcttaccga cctgaacagc cgaaatccac aagtggcttc gcgcctgatt
     5701 gaaccgttga ttcgcctgaa acgttacgat gccaaacgtc aggagaaaat gcgcgcggcg
     5761 ctggaacagt tgaaagggct ggaaaatctc tctggcgatc tgtacgagaa gatcaccaaa
     5821 gcactggctt gataaataac cgaatggcgg caatagcgcc gccattcgga gaatttaccc
     5881 ctgttttctc aggcgagttt caaattcacc acgctgcatc acccgctgca atacttccgc
     5941 ttccagctct gctaatcgta ccgaccctaa ccggcgcgga cgggggatat ccaccgtcaa
     6001 atccagacca atttttccct cttcaattaa caacacccgg tcagccatcg ccaccgcttc
     6061 gctcacatca tgcgtcacca gcaagacggt aaagccgtgc tcctgccaga gtgatacaat
     6121 caaatcctgc atctcgagtc gcgttaaagc gtccagcgca ccgagtggtt catcaagcaa
     6181 caataaacct ggtcgatgaa tcagcgccct tgccagcgcc acacgttgtt tctgcccgcc
     6241 agaaagtgcg gcaggccatt cccccgcgcg attctccagt cctaccgcag ccagagcccg
     6301 acgcgcggca tcgcgccact ggcctttaag gcctaaccca acgttatcaa tcaccgattt
     6361 ccacggcagc agacgcgcgt cctgaaacat cattcgcgta tcatcctgaa tctcagccag
     6421 cggtgtggtg cccgctaaca cgtcgcctgc ggttggcgtt tccagacctg ccagcaggcg
     6481 cagcagggta cttttgccac caccgctgcg gcccaccacc gccacaaact gacctgctgg
     6541 aatatgtaaa tccagttggt tcaggacgat attttccgcg taatgtttgc ttactgcatt
     6601 gagcaacaat ggtgtgccct ggttcagacg agcagtattc ataccgtggc ctccttcaaa
     6661 tgataagccg ggttccagcg caaccagaga cgctctaaca gctgcgcgct gacgtcagca
     6721 agtttgccga gcagggcgta aagaataatg gcgactacca ccacgtccgt ttgcagaaac
     6781 tcccgcgcat tcatcgccag ataaccaatg ccggaattgg cagaaatagt ttcggcaacg
     6841 atcagcgtca gccacatcag gcccaaagca aaacgcacac cgaccataat tgagggcagg
     6901 gcaccaggca ggatcacatg gataaacagc ggcatgccgg ataatccata gctacgtgcc
     6961 atctccacca gctcacggtc gatattacgg atgccatgcc aggtgttgat ataaatgggg
     7021 aatagcgtac cgagcgccac cagaaagatt tttgcggatt catcaatgcc aaaccacaaa
     7081 atcaccagcg ggatcagcgc cagatgcggc acgttgcgca acatctgaat tgaggtatcc
     7141 agcagtcgct caccccagcg tgacagcccg ctaatcagcc ccagaattaa tcccagcgat
     7201 ccgccaattg aaaaaccaat cagtgcacgc caggagctga tcgccagatg ctgccacagt
     7261 tcgccgctgg cagagagcgt ccagaacgcc atcaccactc cttccggtga gggcaaaata
     7321 cgcgtcgata gccagccaac cgaggaggcc agttgccaca ccgccacgat gcccaccggt
     7381 aaaaaccagg gggcaacgcg caataaccac ttcttcactg gcgttgccat catcgtgttc
     7441 cttagctttg cgcgacgtta cgggggataa aatcattcgc caccgcttcg ccttgtggat
     7501 tcagcggttg tggctgggga atttccggga tggcgacatc cagatgtggg aacagtaact
     7561 cgccgacccg atacgcttct tccagatgcg ggtagcccga aagcacaaaa ctgtcgatgc
     7621 caagtgcagc gtattcgttg attcgcgcgg cgaccgtagg accatcgccc accagcgctg
     7681 tcccggcacc gccgcgcact aagccaacac ccgcccataa attggggctg atctccagat
     7741 tgtcgcgctt gccgttatgt agcgccgcca ttcgctgttg tcctacggaa tccgttcggg
     7801 cgaatgaggc ctgtgctttg gcgatagttt catcatcaag atgcgagatt aaccgctcgg
     7861 cggcctgcca cgcttcgtcg ttggtttcac gaacaatcac atgcagacga ataccgaaac
     7921 gaattttgcg tccatgcgcg gcagctttcg cccgcacgtg ttcgattttc tcttttacca
     7981 gtttcggtgg ttcgccccag gtaaggtaga gatcaacttg ttctgccgcc agctcctggg
     8041 cgacatctga cgatccgcca aagtaaagcg gaggataagg ctgttgaatc ggcgggaaga
     8101 gcagtttggc cccgcgtaca tgaatatgtt tgccgttgaa atccacagtc tcgccaagca
     8161 acaaccgccg ccagacctgg gtaaattctg ccgaggcttc gtaacgctcg ctatgatcaa
     8221 ggaacacgcc gtcgcctgcc agttcttggg gatcgctgcc tgtgaccagg ttaaacaacg
     8281 cacgtccgtt agagagacgg tcaagcgtgg cggcctggcg ggcggcaacg gtaggtgagg
     8341 ttacgctggg gcgcagggcg acaagaaact tcagccgttg tgtcaccggg atcatcgacg
     8401 ccgccaccag ccacgcatct tcacacgatc gccccgtcgg gatcagcaca ccggtatagc
     8461 ccagacgatc cgccgcttgc gcaatttgtt gcagataacc gtgatcaacc ggacgtgaac
     8521 cttcttccgt tcccagataa tgcccgtcac cgtgggtcgg taaaaaccag aacatattca
     8581 gactcataat tgttttcctt ccagttgagt gggctgccag atgcgctggc gaatatcgac
     8641 ttttttcggc accagacggt tttcataaaa cagatctgcc gtttgctgct gtaaggcggc
     8701 aacttcagcg ctcaacggtt tgatggtggt gggaggacga tgatccagat acgaggcaat
     8761 aaccggtgcc ggtaagccca tcgtttttgc cagtaaagcg atgctttgtt cgcgctggct
     8821 gcgggttaac gcatcggcct cactaaaggt tgtcagcaca ccctgaataa aagcgccgtt
     8881 tttttctgca tacggacgag ctgccagata aaacgatcca gtttgattga gatcggtgcc
     8941 gtctttcagc acccgcacgc cgccctgtaa taatgcagcg gagtagtagg gatcccagat
     9001 agcccaggcg tcaacgttac cttgctggaa cgcggcgcgg gcatcggcag gcgtcaggta
     9061 agtgggctga atatccgtga atttaagccc ggcctggcga agggctcgca gtaaaagatt
     9121 gtgcgaactg gaacctttct gaaaggcaac tttgtgacct ttaagatcgg ctacggtttt
     9181 gatcgggctg ttttctgcca ccagaatcac ttcggctttg ggcttcggtg gctcgacgcc
     9241 cacgtatacc aaatcagccc cggcagcctg ggcaaagatt ggcggaatat ccccggtact
     9301 gccgagatcg atactgccaa cgtttaacgc ctccagcatc tgtgggccag cggggaactc
     9361 gacccaggag atttttgttt gcggatagcg tttttccagt aactggtggc tttttgccag
     9421 caccatacca atactgcctt tttgatagcc tatacgtaac gcttcaggcg aggattctgc
     9481 agcaaccgca atcgtagaga cgctaagtaa acccgccagc accagtttaa tgatcttacg
     9541 catgggcatt acctcgcaaa gacagtaggt caggaacctg aacatcgcgg cggtgcaacg
     9601 cctgccagaa agtttctagc gcggtatcaa gacgagtttg cagatttggc gtgaactggg
     9661 gtttgtgttg gtaatcaatt acttgcgagt catcggcaaa cacgccgtgc aggatctcct
     9721 gggctttcag cgtacttaaa actggcttaa gggcataatc gaccgccagc agatgggcca
     9781 cggtgccgcc tgtcgccagc ggcagcacca ctttgccttg caacgcacgt tctggcagca
     9841 ggtcgagcag ggttttcagt gcaccggaat aggcggcttt atacacaggc gtggcgacaa
     9901 tcagcccatc ggcctgttgc agctgttcgg tgaaggtctt gagtgccgga ctatcgaaac
     9961 gagcataaag cagatcttcc gggacgaagt tttgcaaatt ccagtgataa acctctacat
    10021 ccaggccatt tagtttttcc cgcgcatatt ccagcaagga gctggagcga gaaggaaatc
    10081 gaggactacc cgccagggtg atgacacgca tactctctcc ttataaccaa ttgttcttgt
    10141 tttgttgaca ttgataacaa ttcggtcagt ctgacagagg gcgtccgcga gtcgaaatga
    10201 tttatctgag gaagaaaagc caaaaaaagc ataaaaaaag cgcaataaga aaacgtttgc
    10261 tatttattgc cgcaggtcaa ttcccttttg gtccgaactc gcacataata cgcccccggt
    10321 ttgcacaccg ggaatccagg agagttcatg tactacccct tcgttcgtaa agcccttttc
    10381 cagctcgatc cagagcgcgc tcatgagttt acttttcagc aattacgccg tattacagga
    10441 acgccgtttg aagcactggt gcggcagaaa gtgcctgcga aacctgttaa ctgcatgggc
    10501 ctgacgttta aaaatccgct tggtctggca gccggtcttg ataaagacgg ggagtgcatt
    10561 gacgcgttag gcgcgatggg atttggatcc atcgagatcg gtaccgtcac gccacgtccc
    10621 cagccaggta atgacaagcc gcgtctcttt cgtctggtag atgccgaagg tttgatcaac
    10681 cgtatgggct ttaataatct tggcgttgat aacctcgtag agaacgtaaa aaaagcccat
    10741 tatgacggcg ttctgggtat taacatcggc aaaaataaag atacgccggt ggagcagggt
    10801 aaagatgact atctgatttg tatggaaaaa atctatgcct atgcgggata tatcgccatc
    10861 aatatttcat cgccaaatac cccaggatta cgcacactgc aatatggtga agcgctggat
    10921 gatctcttaa ctgcgattaa aaataaacaa aatgatttgc aagtgatgca ccataaatat
    10981 gtgccgatcg cagtgaagat cgcgccggat ctttctgaag aagaattgat ccaggttgcc
    11041 gatagtttag ttcgccataa tattgatggc gttattgcaa ccaataccac actcgatcgt
    11101 tctctggttc agggaatgaa aaattgtgat caaaccggtg gcttaagtgg tcgtccgctt
    11161 cagttaaaaa gcaccgaaat tattcgccgc ttgtcacagg aattaaacgg acgcttgccg
    11221 attattggtg ttggcggcat tgactcggtt atcgctgcgc gtgaaaagat tgctgcgggt
    11281 gcctcactgg tgcaaattta ttctggtttt atttttaaag gtccgccgct gattaaagaa
    11341 atcgttaccc atatctaatt atttcttcaa attcctcttt ataagataat cagggcttta
    11401 ttttcagccc tggttgtttt atattcatcg ctgttgctta tttagacatt ttgtactttt
    11461 attgttgagg ttattaagcg aagcgacaat ggattgtgtc gctgcggttt tataaggttg
    11521 gggagtggca gatgcgaatt aaaccagacg ataactggcg ttggtattac gatgaagagc
    11581 acgatcgaat gatgctcgat ttagccaatg gtatgctatt tcgctcacgt tttgcgcgca
    11641 aaatgttgac tccagatgcc ttttctcccg caggtttttg cgttgatgac gccgcgctct
    11701 atttctcttt tgaagaaaag tgccgcgatt ttaatttatc taaagaacaa aaagccgaac
    11761 tggtgctgaa tgcactggta gcaattcgtt atctcaaacc gcaaatgccg aaaagctggc
    11821 attttgtatc tcatggtgaa atgtggatgc ccatgccggg cgatgcggcc tacgtttggt
    11881 tgagcgacac tcacgagcaa gtgaatttac tggtcgttga atctggcgag aatgccgcgc
    11941 tatgtctact ggcacaaccc tgcgttgtta ttgcgggtcg cgcgatgcag ttgggcgacg
    12001 ccattaaaat catgaacgac aggctgaaac cgcaggttaa tgttgacagc ttcagcctcg
    12061 aacaggcagt ctaacgcgcc agcttaagcg cagtcttcgg tacacaacta cagcaaagaa
    12121 tagtgccatc atcgcctgtt gctgattttt ttagcggcgt gacttcgcct tctaaaagct
    12181 gaacacggca acttccacaa atgcctgcgc ggcaagaata agggatacga attccctgat
    12241 tttctaattg ctccagcagc acctgttggt tatttccacg aaatgcctgt ccctgccagt
    12301 caatatctac gtttgcgtcc ggttgttgcg tgatgttggc agtaccatca gcggcacctg
    12361 cgccgtaaat tttagctgga gccgttgcca gaatttccac ctcatcgcca acgcgaatca
    12421 cgccgctatt acgggcaatt aaattctgac caaaatcgac atcgccgtta tcctgggcag
    12481 tacggaaaga ttgcagcgtt tttaatggtt cgtctgccgg atgtttttgc cctttttctg
    12541 ggctgacggt ggtgaaaata cagcggctac aaggcttaac cacatcaaac acgacatcac
    12601 caatgcgaat cactttccag ctatcttctt cccaggccga tgcaccggaa accaccagat
    12661 tggggcggaa ttgctccatt tttacactgg ccggacaacg ttgttggaga gcacgtaacg
    12721 aggcctcgtt agcaagaagg taaggatagc catcagcaaa tgacaggggt acagtgttgt
    12781 ggcgtttcac gcgccgggtc atttgtggcc ccacccagcg taattgcact tcgcgggaga
    12841 aaaatccact tagccatttg ttgatcgcat ctggcgcaat tcgcgcggta aaatgtgtgc
    12901 cccaaacttc ggttggtgcg tcttgtgtgg cgaaatcagc aaaacgaaca tatgcgctac
    12961 tgccatctgg tgcggtgaga tgcaagccat catgcacggg tgaaggagta aaccgtacca
    13021 ttagcggaaa ctggcgagcg gtaataaacg taccgtcagg ttccgtgatc ataaagatgc
    13081 gatcgaaggc cagaccactg acatctgcca gggtatgtgt aagaccaatg ccgcgcatcg
    13141 atttaacagg atgaataaaa agccgggtta atgtcgccac ggtgcggtcc tcaaatgaaa
    13201 ataagccctc aactttatga catgacgcgc ttattagcta taatgcgcaa caattttctt
    13261 agagtaaaag tgacgatatg aattctctgt ttgccagtac ggcccgtggg ctggaagagc
    13321 tgttaaaaac tgaactggaa aacctggggg ccgttgaatg ccaggtggtt cagggtgggg
    13381 tccatttcaa gggcgacaca cggcttgttt accagagcct gatgtggagc cgcctggcct
    13441 cgcgtattat gttgccgctg ggcgagtgta aggtttacag tgatttagac ctctatctcg
    13501 gcgttcaggc gatcaactgg acagagatgt ttaatcctgg cgcgaccttc gctgtccatt
    13561 tcagtggttt aaatgacacc attcgcaaca gccagtacgg cgcgatgaaa gtgaaagacg
    13621 cgatcgtcga tgctttcacg cggaaaaatc tgccacgtcc caatgttgat cgcgatgcgc
    13681 cggatattcg cgttaacgtc tggctgcata aagaaaccgc caatatcgcc cttgatctca
    13741 gtggtgatgg cttgcatctg cgtggctatc gtgatcgtgc tggtattgca ccgatcaaag
    13801 aaaccctggc agccgcgatt gtgatgcgat ccggctggca gccaggaaca ccactgctcg
    13861 atccaatgtg tggttctggt acgttgctga ttgaagcagc gatgctggcg accgaccgcg
    13921 caccgggctt gcaccgtggc cgttggggct ttagcggctg ggcgcagcat gatgaagcta
    13981 tctggcagga agtgaaagcg gaagcgcaaa ctcgcgcccg taaaggcctg gcagagtata
    14041 gctctcattt ttacggttcg gacagcgacg cccgggtgat tcaacgtgcg cgcactaacg
    14101 cccgtcttgc ggggattggt gaactgatca cctttgaggt gaaagatgtc gcgcaactga
    14161 ccaatccgct gccgaaaggg ccgtacggta cagtgttgag caacccgcca tacggtgaac
    14221 gtctggacag cgaaccggcg ctgattgcgc tgcatagtct gctgggccga atcatgaaaa
    14281 accagttcgg tggctggaat ctctctttgt ttagtgcctc gccggatctg ctaagctgcc
    14341 tgcagctgcg tgcagacaaa cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga
    14401 aaaactacca cgttgccgaa agcacaccag acagcaaacc ggcaatggta gcggaagact
    14461 acgccaaccg cctgcgtaag aacctcaaaa aattcgagaa gtgggcacgt caggaaggga
    14521 ttgaatgtta tcgcctgtat gatgccgatc tgccggaata taacgttgcc gttgaccgtt
    14581 atgccgactg ggtggtggtg caggagtatg cgccgccaaa aactattgat gcgcacaaag
    14641 cgcgtcagcg tctgttcgat attatcgctg caaccatttc ggtactgggg attgcgccaa
    14701 acaaactggt gctgaaaacc cgtgaacgcc agaagggcaa aaatcagtac cagaaactgg
    14761 gcgagaaggg cgagtttctt gaagttaccg aatataacgc tcacttgtgg gtgaacttga
    14821 cggattacct cgataccggt ctgttcctcg atcaccgtat cgcccgtcgt atgctcggtc
    14881 agatgagcaa aggcaaagat ttcctcaacc tgttctccta taccggtagc gccaccgtgc
    14941 atgcgggatt aggcggcgcg cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg
    15001 agtgggcaga acgcaacctg cgtctgaatg gcctaaccgg gcgtgcgcat cgcctgattc
    15061 aggccgattg cctggcgtgg ctgcgtgagg caaatgaaca gttcgatctg atctttatcg
    15121 atccgccaac cttctctaac tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc
    15181 atctggcgct gatgaaagat ttgaaacgtc tgctgcgtgc aggtgggacg atcatgttct
    15241 cgaacaacaa acgcggcttc cgtatggatc tcgacggcct ggcgaaactg ggactgaaag
    15301 cacaagaaat tacgcaaaaa acgctctccc aggatttcgc ccgtaaccgc cagatccaca
    15361 actgctggct gattaccgca gcctgaaagg aatagtaatg tcattaatca gtatgcatgg
    15421 cgcatggctg tcgttcagcg acgcgccgct tctcgataac gcagaactgc atatcgaaga
    15481 taacgaacgt gtttgtctgg tgggtcgtaa cggcgcaggc aaatcgacgt taatgaagat
    15541 cctcaaccgt gaacaggggc tggatgacgg tcgcattatt tacgagcaag atttgattgt
    15601 agctcgtctg caacaggatc cgccgcgtaa cgttgagggt agcgtttatg atttcgttgc
    15661 cgaaggcatt gaagaacaag cggaatacct gaaacgctat cacgatattt cgcgcctggt
    15721 gatgaacgac ccgagcgaga aaaatctcaa cgaactggcg aaggttcagg aacagctgga
    15781 tcaccacaac ctgtggcagc tggaaaaccg catcaacgaa gtgctggcgc aactgggatt
    15841 agatcctaat gttgcgctgt cgtcgctttc cggtggctgg ttgcgtaaag cggcattagg
    15901 acgcgcgctg gtgagcaatc cgcgcgtatt gctgctcgac gaaccgacta accacctgga
    15961 tattgaaacc atcgactggc tggaagggtt cctcaaaacc ttcaatggga caattatttt
    16021 catttcccac gaccgttcgt ttatccgcaa tatggcgacg cgcattgttg atctcgatcg
    16081 cggcaagctg gtgacctatc cagggaatta cgaccagtac ctgctggaaa aagaagaagc
    16141 cctgcgcgtg gaagaattac aaaatgccga gttcgatcgc aaactggcgc aggaagaggt
    16201 gtggatccgc caggggatca aagcacgtcg tacccgtaat gaaggccgcg tacgcgccct
    16261 gaaagcaatg cgtcgtgagc gtggtgaacg tcgcgaagtg atgggtaccg caaagatgca
    16321 ggtggaagaa gccagccgct ccggtaagat cgttttcgaa atggaggacg tttgctacca
    16381 ggttgacggt aagcaactgg tgaaagattt ttctgcccag gttctacgcg gcgacaaaat
    16441 tgccctgatt ggtccgaatg ggtgcggcaa aaccacgctg ctgaaactaa tgctcggtca
    16501 gcttcaagca gacagcgggc gtattcacgt tggcaccaaa ctggaagtgg cttatttcga
    16561 tcagcaccgc gcggaactgg atcccgataa aacggtgatg gataaccttg ccgaaggtaa
    16621 gcaagaggtg atggttaacg gcaagccacg ccacgtattg ggctatttgc aggacttcct
    16681 gttccatcca aaacgggcga tgacgccggt acgtgcgctt tctggcggtg agcgtaaccg
    16741 cttgttgttg gcgcgtttgt tcctcaaacc aagcaactta ttgattcttg acgaaccgac
    16801 caacgatctt gatgtcgaaa cgctggaact gctggaagaa ctgatcgaca gctatcaggg
    16861 cacggtattg ctggttagcc acgatcgtca gtttgtcgat aacaccgtta cagaatgctg
    16921 gatcttcgaa ggcggcggta aaattggtcg ttatgtcggc ggttatcatg atgcccgtgg
    16981 tcagcaagaa cagtatgtgg cgctcaaaca gcctgcggtg aaaaaaagcg aagaagccgc
    17041 cgcgccaaaa gcagaaactg taaaacgcag cagtagcaaa ctaagctata aattgcagcg
    17101 tgaactggag ctgctaccgc aattgcttga agatctggag gcgaagctgg aagctttaca
    17161 gacgcaagtg gcggatgctt ccttcttcag tcagccgcat gagcagacgc aaaaagtact
    17221 cgctgatatg gctgctgcag agcaggagct ggagcaagcc tttgaacgct gggagtatct
    17281 tgaagcgtta aaaaatggtg gctgatcgca aaatagtcga tttaaacaga gactgctgac
    17341 aacggtaaaa ttgcctgatg tactgggttt atcaggcatg aaaaccaaca ctttgttagt
    17401 catctgcaaa atagcgcatc attaaggagt accaatgtgc gaacatcatc atgccgcgaa
    17461 gcacatcctg tgctcgcagt gtgacatgct ggtggcgtta ccgcgccttg agcatggtca
    17521 gaaagcggca tgtccccggt gtggcacaac gttaaccgtg gcgtgggatg cccctcggca
    17581 gcgtcccacc gcctatgcgt tggctgcact gttcatgctg ttgctgtcca acttgtttcc
    17641 ttttgtgaat atgaacgttg caggagttac cagtgaaatt acattactgg aaattcccgg
    17701 cgtgcttttt tctgaggact acgccagcct cggcaccttt ttcctgttgt ttgtgcaact
    17761 ggttcccgcg ttttgtctga taaccattct gttactggtg aatcgcgcgg aattaccggt
    17821 ccgtttaaaa gagcaactgg cacgggtgct ttttcaactc aaaacctggg gaatggcgga
    17881 gattttcctc gcgggtgtgc tggtcagttt cgttaaactg atggcttacg gcagcattgg
    17941 ggtaggcagc agctttctcc cctggtgttt attttgtgtc ctgcaactgc gcgcttttca
    18001 gtgcgttgat cgtcgctggt tatgggacga catcgccccg atgccagaac tgcgccagcc
    18061 gctaaaacca ggcgtcacgg ggatacgcca ggggctgcgt tcgtgttcct gttgtacggc
    18121 aatccttcct gctgatgaac ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg
    18181 tagaaacagc ctgcaatgga cactcgcgct gcttgtaacg tctatcatgc tgtatcttcc
    18241 ggctaatatt ttgcccatca tggtgacgga tttattaggc tcgaagatgc cgtcgacgat
    18301 tctcgctggg gtcattctgt tatggagcga aggctcttat cccgtcgctg cggtgatttt
    18361 tctggccagt attatggtgc caacgttaaa gatgatcgcc attgcgtggc tgtgttggga
    18421 tgccaaagga cacggcaagc gcgacagtga aagaatgcat ttgatttatg aagttgttga
    18481 gtttgtaggc cgctggtcga tgattgacgt tttcgttatc gcggtgctct cggcgctggt
    18541 gcgtatggga ggtttaatga gtatttatcc ggcaatgggt gcattaatgt ttgctttagt
    18601 cgtcataatg acaatgtttt ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca
    18661 acctgaatca gagcatgagg agtcctgaca gtatggaatc taataatggg gaagccaaaa
    18721 tccagaaagt gaagaactgg tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg
    18781 gggcctgggt tcttttttat cattacagcc atcagggacc ggaagtgacc ctgatcaccg
    18841 cgaatgcgga aggaattgaa ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg
    18901 gcgtggttga aagcgccaca ctggctgatg atttgacgca cgttgaaatc aaagcgcggc
    18961 tgaattccgg tatggaaaaa ttgctgcata aagataccgt cttttgggtg gtgaaaccgc
    19021 agattggtcg agaagggatt agcggcctgg gaacgctgct gtctggggtt tatatcgaac
    19081 tgcagccagg cgcgaaaggc agcaaaatgg ataaatacga tttgctggac tcgccaccgt
    19141 tggccccgcc tgatgcgaaa ggtatccgtg tgcttctcga tagcaaaaaa gccgggcagc
    19201 tctcgccagg ggatccggtg ctgttccgtg gctatcgggt aggttcggtt gaaaccagca
    19261 ctttcgatac gcaaaaacgc aatatcagct atcaactgtt catcaatgca ccttatgacc
    19321 gactggtgac cagcaatgtt cgtttctgga aagatagtgg cattgcggtt gatctgacgt
    19381 cagcgggaat gcgtgtggag atgggctcat tgacaacgct gcttagtggc ggtgtcagct
    19441 ttgatgtgcc ggaaggtctg gatttagggc agccagtggc accgaaaaca gctttcgttt
    19501 tgtatgatga tcagaagagc attcaggatt cgttgtacac cgatcacatt gattatctga
    19561 tgttctttaa agattcggta cgcggtctgc aaccgggagc tccggtagaa ttccggggta
    19621 ttcgcctggg taccgtaagc aaagtgccat tctttgcgcc gaatatgcgt cagacattta
    19681 acgatgatta ccgtattccg gtactgattc gtatcgagcc agagcggctg aaaatgcagc
    19741 ttggcgaaaa tgcggatgtt gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg
    19801 gatcgctgaa aaccggaaac ctggtcactg gcgcactgta tgttgatctc gatttctatc
    19861 caaatacgcc tgcaataacc ggtattcgtg aatttaatgg ttatcagatt atcccgaccg
    19921 tcagcggcgg cctggcgcaa atccagcaac gactgatgga agcgttggat aagatcaaca
    19981 aactgccact gaatccgatg attgaacagg caaccagtac gctttctgaa agtcagcgca
    20041 caatgaaaaa cctgcaaacg acgctggata gcatgaacaa gatcctcgcc agccagtcga
    20101 tgcagcagtt accgacggat atgcagtcaa cgttgcgtga attgaatcgc agtatgcagg
    20161 gcttccagcc tggttccgca gcctacaaca agatggtggc ggatatgcag cgccttgatc
    20221 aggtgttgcg agaactgcaa ccggtgctga aaacgctcaa cgagaagagt aacgcgctgg
    20281 tatttgaagc gaaggacaaa aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg
    20341 gctagtgacg attgcggcac tgtggctggc cggatgcagc tccggtgaaa ttaataaaaa
    20401 ctattaccag ttacctgtgg tgcagagcgg tacacaaagc accgccagcc agggtaatcg
    20461 cctgttatgg gttgagcagg tcgctgttcc tgactatctg gcggggaatg gtgtggttta
    20521 tcaaaccagt gatgtgaagt atgtgattgc caacaacaac ttgtgggcca gcccgttgga
    20581 tcaacagttg cgcaacaccc tggttgccaa cctgagtacg caactgcccg gctgggtggt
    20641 tgcctcccag cctctgggaa gcgcccagga cacgctcaat gttaccgtca cggagtttaa
    20701 cggtcgctat gatggcaagg tcattgtcag tggtgagtgg ctgttgaacc accagggaca
    20761 actgatcaaa cgtccgttcc gtctggaagg agtgcaaact caggatggtt acgatgagat
    20821 ggttaaagtg ctggcccgtg tctggagtca ggaagccgct tctattgcgc aagagataaa
    20881 gcgtctacct taatcataaa gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc
    20941 ggtttttttg tttctaacgt gcggaaaaag ttgttcctct tcacattttt tgtacaaccg
    21001 acatgcccgt gtagctcaca aatatgacag tggcgtgaat tttgcgcatt gacggcagtt
    21061 atgattcgcg gtattgctta actgtggttg catatttagt aatcactgtt ttcttttcca
    21121 ccagaaacca gtatgaggga aacgaggcat gaagagacaa aaacgagatc gcctggaacg
    21181 ggcacatcaa cgtggttatc aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta
    21241 tcagacgctg aatcaaaggt cacaatggct gggaggctgg cgagaagcca tggcggacag
    21301 ggtagtaatg gcctgattct gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc
    21361 ctttttatac tctgtctgat tattatcaga aggcagacgt atcctggaac agaccgactt
    21421 tcaggtcgtt ggcggtatag atcaaacgac catcaaccag cacttcgcca tcagccaggc
    21481 ccataatcag acgacggtta actatgcgtt taaagtgaat acgataggtc acttttttcg
    21541 ccgtcggcag cacctgacct gtgaatttca cttcacctac gcccaacgcg cgacctttac
    21601 cttcgccgcc cagccagccg aggtagaacc ctaccagctg ccacattgcg tccaggccca
    21661 ggcatcccgg cataaccgga tcaccaatga agtggcatcc gaagaaccac agatccggat
    21721 tgatatccag ctctgcttca acataccctt tgtcgaagtt accacccgtt tcggtcattt
    21781 tgaccacacg gtccatcatc agcatgtttg gtgctggcaa ttgcgggcct ttagcgccaa
    21841 acagttcacc gcgaccagag gcaagaaggt cttcttttgt ataggattcg cgtttatcta
    21901 ccatgttctc tgtaagcctt attttattga agcacgcagg atagctaaca cgtgtacgct
    21961 gaacaagtcc gatcagttcg gaataaacca gttcagccaa cgtaatggcc atggaaaacg
    22021 gtgacgtcct tcctgttgcg atgcttgtgc gatacgttcc tggatggttt gcatcagcgt
    22081 cgtctggcct tcgccatccc acaccagatt taataataac ggcagtgcgt cagtcacatc
    22141 gtctactgcc cagatggtga atttgccttc ttctaccgct ttcaccagtt cactgtgaag
    22201 acttaaatgg cgaacgttag cagtggggat aatgacacct tgtttccccg ttaactcacg
    22261 ttgctggcaa atagcaaaga agccttcgat tttctcattt aaaccaccga ctggctgggc
    22321 gcgaccgaac tgatcgactg aacctgtgat agcgatactc tgattcaccg gcacatcggc
    22381 gagggcgctg atcagagcgc agagttcagc catcgaggca ctatcgccat ccacttcact
    22441 gtatgactgc tcaaatgtca gcgatgctga gaaggggatc tgttgctcaa gctgtagttc
    22501 cgacatcagg aacgcttgca tgatcatcat ccctttcgca tggatattgc cgccaagctc
    22561 cgctttgcgt tcgatgtcgg tgaattcacc atcgccaata tgcacaacgc agctaatgcg
    22621 agaaggttcg ccaaaagcgc gtggatgacc cggaaattca atgaccgaaa gggcgttaat
    22681 ttgcccgatg cgttcgcctt cggtttcaat caggatttgc tcctgaagga tctcatcctg
    22741 catgcgttca gcgaggaaac cttcgcgcca ttcacgctgc tgtagcatta agtttagctg
    22801 ctcgccggag aaggtgtcgc catcacacag ggaggcgacc tcttgacact ggcggaggat
    22861 ccactgcggg ctaagcggaa gtgtttcttg ttcaccagtg tagcgtgctg cttcgcggat
    22921 aagtaccggc caggcatccg ctcccggtgc tggtaagtga ttatgtctgg cggtaaatgt
    22981 cacccagcga caccactggc ttactgactc cgcatcgaca atctgcagag tatcttcaaa
    23041 ttcgctataa atagcctgct ctgaaagctc tggctccatc tcctggaaat cagccaatga
    23101 ttcgcgttcg cctaccagaa tgactttcag cttcaatggc atcgaaggca cagagacggg
    23161 gagagggcgc gactcatcaa acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt
    23221 cagccgcatc cacagcagag gttgcgccag cagtgtacgc aaagagatga tgagaatacc
    23281 gccatttgcc tgatgcacca gaccaggctg cagggtaata tcgccattaa actgacgcag
    23341 gcagccaaag agttgctctg cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc
    23401 aaaattatca tctgcactca ctgcgtgacg taagcggatg gtgtggccgg aaacctcata
    23461 gtgaccgccc accagttggc ctgcatcgct ttgtagcgta cgcgcggcat cagcaataag
    23521 attcagatac tcagactctt ccggggcctt cgccagcata aaagaggagg atgctcgcgt
    23581 atgcagcaac tgctccagcg caaattgcag tcgcggttga gtatcactga ataaaggatc
    23641 gttttcgtca atcaaatgtg gctgagcaaa tatttcctga tagctatcgg tatcaggaac
    23701 caggtcacgc catgcaagtt tcgtaatggt caaagttgat gttttttagt ctgttgtcaa
    23761 agccgcgatt ataccgtaac cggcactgca gcacacgtag aaagcaacga caatacgcct
    23821 ggcatgggcg ttaaagctca caggatggag attcttttct tcactggcct aaaaagctga
    23881 tattctgtaa agagttacac ggtaacattg agatcgctat gaaatatcaa caacttgaaa
    23941 atcttgaaag cggttggaaa tggaagtatc tggtgaagaa gcaccgcgaa ggggagttaa
    24001 tcacccgtta catagaagcc agtgccgccc aggaagccgt cgatgagttg ctctcgctgg
    24061 aaaatgaacc cgtgctggta aatggttgga ttgacaagca tatgaatccg gaactggtca
    24121 atcgcatgaa gcagaccatt cgggccagac ggaagcgcca ttttaatgcg gaacaccagc
    24181 atacgcgcaa aaaatcgatc gatctggaat ttatcgtctg gcaacgtctg gctggccttg
    24241 cgcagcgtcg cggtaaaacg ctttctgaaa cgattgttca gctgattgaa gatgcggaaa
    24301 acaaagagaa atacgcgaat aaaatgtctt ctttgaagca ggatctgcag gcattgctgg
    24361 gtaaggaata actgtcaaaa gtcagttcaa tttacgcaag gcgaaaaaaa accccgcagc
    24421 agcggggttt tttaccagac gagaacttaa gcctgcggct gagttacaac gtctttgatg
    24481 cctttaactt cgatctctac gcgacgatcc ggagccaggc aatcgatcag tgcagcacgc
    24541 tgtttcacgt tgtcacaggt gttgccagta accgggttgg attcgcccat accacgtgcg
    24601 gagattttgt ctgccgggat acctttagag atcaggtaat caacaacgga ctgagcacga
    24661 cgctcggaca gagcctggtt ataagcgtca gaaccgatgc ggtcagtgta acccagaaca
    24721 actacggaac cgtctttcgg atccaggttg ctcagctggc tgtacagctg atccagagca
    24781 gcctgacctt ccggtttcag ggttgctttg ttgaaggtga acagaacgtc agacttcaga
    24841 gtgaagtgct tggtctgtac ttccggtgcc ggagctggag ccggagcaac tactggagct
    24901 gcttcgccct gaccgaaacg gtaggaaaca cccaggctca gcatgccgtt gtccggacga
    24961 gtgccgatgg tgtgtgcatc accgatgttg ttggtccact ggtattccag acgggtagcg
    25021 atttcgggag tgatcgcata ctcaacaccg ccagcgaaga ccggagaaac gccggtgtcg
    25081 tggtctttaa aggatgcgcc accaggtacg ttagccttgg tgtctgcacg ccataccata
    25141 ccacccagac gagtgtagat gtccagatcg tcagtgattg ggtaacccag tttagcggtc
    25201 agctgaacgc cctgagcttt gtatgcgccg ttgatgttgt cgcctttgta cggcatacga
    25261 cctaaccagt cgtaacccat ttcaaagcca acatacgggt taacctggta accaccaaaa
    25321 gcacctgcac ccagttggtt ttcgtgggtc ggaccattgt taggaataaa accagtgtca
    25381 tggtactggg accagcccag tttagcacca gtgtaccagg tgttatcttt cggagcggcc
    25441 tgcgctacgg tagcgaaacc agccagtgcc actgcaatcg cgatagctgt ctttttcatt
    25501 ttttgcgcct cgttatcatc caaaatacgc catgaatatc tccaacgaga taacacggtt
    25561 aaatccttca ccggggatct gctcaatatt aactctaccg atatcttcgg cttatgccga
    25621 gcacccctgg cgatgtaaag tctacaacgt agttgaaaac ttacaagtgt gaactccgtc
    25681 aggcatatga aaaaaaagtc ttgtataagg tacgtttaat cttttttgtc agcgacaatt
    25741 tacagaagaa aatcgcggaa accgcttcag acaagcctcc gcaaggaaaa ttagtcacga
    25801 ctgaaagcat tggctgggcg ataaaaaaag ttccaggatt aatcctaaat ttacttaatg
    25861 atacaaatta gagtgaattt ttagcccgga aagttgtctc gttgcgtgag aggatgcgct
    25921 taccggacgc ataataaacc ccatagcgtt accttcattt gccgcatcaa caagttcagc
    25981 atgctcttct gcagtcaaat catctgccaa ccaaccgatc accacgctgt aattgcccgt
    26041 gcgtaaagcg cgaaccattg actccacggt gtggcaaggg gagagctggc taatctgcat
    26101 tactttcgtt aagggtagcc cagatgcctg aacccattcg cgactcagtt tttgttgcgg
    26161 tgttaaccag agttgccagc gtgattgctg accgagttgc tgtaacaatg gcaacagtag
    26221 aagttgcgtc atcatgggct gatcttcgcg atagacaact tcactgataa gcccggctgt
    26281 agtgttttcc gtagagacac gcgcaatttt acttgctgcg gatgagaacg acgaagaacg
    26341 atgtgcatag cctgaagtgt acataatcaa tccagcccct gtgagttact gtatgaatgt
    26401 acagtacatc cagtgacgac aaagatcaac cctattttcg gaaagagcct cgcaaatttt
    26461 gtcgttggtg gcgggaaaac ataaattaat cttgcccctt aagaataagt tgcctatttt
    26521 cgtagttaac ggatccgtta atgtgaatca ttcttttatg ttatgatttt aaaaggaatt
    26581 ttatgaaaag cctctcctat aagcggatct ataaatcaca agaatacctg gcaacgttgg
    26641 gcacaattga ataccgatca ttgtttggta gttacagcct gaccgttgac gacacggtgt
    26701 ttgcgatggt ttctgatggt gagttgtatc ttcgggcttg tgagcaaagt gcacagtact
    26761 gtgtaaaaca tccgcctgtc tggctgacat ataaaaagtg tggccgatcc gttaccctca
    26821 attactatcg agttgatgaa agtctatggc gaaatcaact gaagctggtg cgtctgtcga
    26881 aatattctct cgatgcagcg ctgaaagaga aaagcacgcg caatacccgg gaaagactga
    26941 aagatttgcc caatatgtct tttcatctgg aagcgattct cggggaggtg gggattaagg
    27001 atgtacgggc gttacgtata cttggggcaa aaatgtgttg gttgcgactg cggcagcaaa
    27061 actgtctggt gacagaaaag attctgttta tgcttgaagg ggccattatc ggcattcatg
    27121 aagctgcgct cccagtggca cgccgccagg agcttgcaga atgggctgac tctcttacgc
    27181 cgaaacagga gtttcctgcg gaacttgagt aatctggcgt tgcagacgac caatctctgg
    27241 cagtaatgca atcaataacc caacttgttg tacgaccaga ggttctttgc tgtctgcccg
    27301 tggttcaagt tgctgcatcc gctgtttcag gccagctaat gcctgattga cgcgttcttc
    27361 atcagcaggt tgatgatgta gcgcgtcatc aacatagcaa acggcgtcat caagaaacgc
    27421 cagaatttca ggattagtta actgctcccg gtgagcaccg agggctgaga tatagctggt
    27481 aaacgtatgg ttaaggcaca gcaaccgaaa cgcggcttcg cgaatttgcg gggtaacgtt
    27541 cggttcgctg gacatatttg ataccaccga cgccagttca gcatcacggt tgtgtgcatc
    27601 gcggcgggca atacgatacg ccagacggtt atcacgcccc tgatggtatt gttccagtat
    27661 ggcatcgaga taccggcagt tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg
    27721 aaacttccag tcaggccaga tgtagctcac tgccgcccac gcaatggcac aaccaatcag
    27781 cgtatcgatt acgcgaggta acgccacttc aaaaccttca cccagcaagt tgaaacacag
    27841 tagcaccaaa agtgtgatga acatcgttgc atgggcgtat tgcacgttac ggaaggcaaa
    27901 aaagagcacg ccggtaataa ccagcagcac cagctgccct tccaatgatg gcacaaacca
    27961 cagcacagga ataccaattg cgatacctac cagcgtacca ataatcctta acttcaggcg
    28021 gtggcgcgtg gcgttatagt ttggctggca gacaaacaaa cttgtcagca agatccaata
    28081 cccgtgatgc attccggtta tctgaatgat ggcgtagccg aagcacaaca ccagcgacat
    28141 tcttaccgca tgacggaaga gggcggattc cggcgtgaag tgacggctaa gacgcagcca
    28201 gatatcactc aatccgtgcg ggctgtcatc agcgagctca ttttcgtcat tattatgggg
    28261 cagtgcctgg gcctgttctg attcaattgt tgccagttgg gcatcaatgg cacgtaaatt
    28321 gttcagtaaa aatcccagtg ttttgagtaa atcagcaggc gcgccgttat cgcgcatccg
    28381 ctccagcgca gcatcaatat gcgtaaaagc gcgctcaaaa tgcggatcat gttgataagg
    28441 ctgacgcagc aaaatacagc gtgacagttg ctggcacgcc tggccctgca tcgacatcag
    28501 ccgctgaaaa cggaacagca cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatattg
    28561 aatatgagaa gagctggcac gctcgtgaat atcctgcgcg acaaagtaat aatgcagtgt
    28621 gcgacgcgtt ccccgttgac cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt
    28681 ctgattcaat gtcgccatca gctgaccgtt ggcgagagcc aaatcgtaca gcggtgcctg
    28741 gctttcatct tcaatatcag gatcaaacat gcgcgacttg agctcaagat aacgcgccag
    28801 ttgttcatag caacgcgcca ggttgtcctg cagcgggcgg accgggaaca gcagatgacc
    28861 aataagtgtc aggacgttgt accagacggc accggccaga agatacatcg gctgctgata
    28921 ccagtgctca tacagtgatg ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa
    28981 ggcaattgtt gcatagcgtt gacccagacc gccgagcaaa atgaagccgc tggtagagag
    29041 cgttaagcca atcgcaaata gccagggcca gggaaacagc aattctactg aggccgaggc
    29101 gataaaaaag cagaacagcg taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc
    29161 gagatcggtc agcgccgctg ccaccatccc aagcgtcagc ggaatcgtca gttttacatc
    29221 acccagccac cacggaaacg ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata
    29281 cagccaggcg ctgttccagg tatagcgttt gagcaaagga cttagcataa aggccatatt
    29341 agtggattat tcaaaacgac gacgtgcatt ggcttcgcgc gcagcttgcg ctgtttctac
    29401 cgataccacg cgacgaccga ccggccatag tgcaatagcg gcaattttaa agttcgcaat
    29461 accgacagga atgccaatga tggaaataca ttgtgcgatg cccgttgcaa tgtgcatcag
    29521 gcataaccac cagccaaaga aaatcagcca gaaaatattc aataccgtac cgccagtatt
    29581 cagcagcaca tttttgccag ccgggttcag ttcatcgaca tgaatagctt cattgccata
    29641 aggcaccaga gacagtttag tgatctccca gcaggatcgt gtcagtggta aggtaaaaat
    29701 cagcacaata ctgaccagag tcgccaacag ccagcccaga gtggtggcaa atccgccaag
    29761 cacaaagttc agaatgttca aaacggtacg cataaaacct cgctttactg tggttttcag
    29821 taatgggcgg caattgtaac gtttttttgg gctggagcac gttttctctg acggttacac
    29881 tgataagaaa taatttcgtg tggatctaca gagtcatgga actgaaagcg acaacgcttg
    29941 gaaaacgtct ggcacagcac ccttacgatc gggcggtgat cctcaatgcc gggattaaag
    30001 tctccggcga tcgccacgaa taccttattc ctttcaatca attactggcg attcactgta
    30061 agcgcggtct ggtatggggc gaactggagt ttgtactgcc ggacgaaaaa gtggtgcgtc
    30121 tgcacggcac cgaatggggc gagacgcagc gtttttacca tcatcttgat gctcactggc
    30181 ggcggtggag tggcgagatg agcgaaattg cgtctggcgt tttacgtcag caactggatt
    30241 tgattgccac gcgcaccgga gaaaataaat ggctgacgcg tgagcaaacc tctggtatgc
    30301 agcaacaaat ccgccaggct ttgtcggcgt taccattgcc ggttaaccga ctggaagaat
    30361 tcgataactg ccgtgaggcg tggcgtaaat gtcaggcctg gttgaaagac attgaaggcg
    30421 ctcggttgca gcataatcag gcgtataccg aagccatgct taccgagtat gcggattttt
    30481 tccgccaggt cgagtcttca ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg
    30541 agcattcttt gttagtgctg gctggtgcag gaagcggaaa aacgtcggtg ctggtggccc
    30601 gtgcaggctg gttgctggcg cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt
    30661 ttggtcgcaa agccgctgaa gagatggacg agcgtattcg agaacgacta cataccgaag
    30721 acattaccgc acgcacattt catgcgctgg cgctgcatat tattcagcag ggcagcaaaa
    30781 aagttccgat agtcagcaaa ctggaaaatg ataccgctgc ccgtcatgaa ctttttattg
    30841 ctgagtggcg caagcaatgc agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc
    30901 tgacggaaga aatgcagtgg tcagtgccag aaggtaattt ctgggatgat gaaaaattac
    30961 agcgtcgtct tgcctctcgc ctcgatcgct gggtaagtct gatgcggatg cacggtggtg
    31021 cacaggcaga aatgattgcc agtgcacccg aagagattcg cgatctgttc agtaaacgta
    31081 tcaagttgat ggccccgtta ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg
    31141 tcgatttttc gggccttatt caccaggcga ttgtgattct ggagaaaggt cgctttatca
    31201 gcccgtggaa gcatattctg gttgatgaat ttcaggatat ctcgccgcag cgtgcagcgt
    31261 tgttagcggc attacgcaag caaaatagtc aaacgacgtt gttcgctgtt ggtgatgact
    31321 ggcaggcgat ttaccgattc agcggggcgc aaatgtcgct caccacggct ttccatgaaa
    31381 actttggtga aggtgatcgc tgtgatttag acacgaccta ccgttttaac agtcgtatcg
    31441 gtgaggtggc aaacaggttt attcagcaga acccaggcca gcttaaaaag ccgctaaaca
    31501 gcttaaccaa tggagacaaa aaagccgtca cattattgga tgaggggcaa cttgacgctt
    31561 tgctggataa gctctctggt tatgccaaac cggaagagcg cattctgatc ctggcgcgtt
    31621 accatcacat gaggcctgcc agcctggaaa aagcggcaac acgctggccg aagttgcaaa
    31681 tcgactttat gaccattcat gccagcaaag ggcaacaggc ggattacgtc atcatcgttg
    31741 gcttgcagga ggggagtgat ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg
    31801 cgctactgcc accggttgag gatttcccgg acgctgaaga acggcggtta atgtacgtgg
    31861 cgctgacccg ggcacgccat cgggtatggg cactgtttaa caaagagaat ccctctcctt
    31921 ttgtcgaaat actgaaaaat ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg
    31981 gcgtttgcca cctgtgcaat attacttcag acggtccgcg agataacgct gataatcggg
    32041 gatcagaata tcgaccgcgt cgttaaaatg cggtgactgg ataatgaagt ctgccgttgc
    32101 cacgttggtg gcgaccggaa tgttccatac cgttgccaga cgcagcaagg ctttcacgtc
    32161 aggatcgtgc ggcacggcat ttagtgggtc ccagaagaaa atcaatacat caattttccc
    32221 ttctgagatc aatgcgccaa cctgctggtc accccccatt gggccactca acatcgcgtt
    32281 gacgttcatg ccggtcgcgc gggaaattaa gttaccggta gtgcctgttg catacagtac
    32341 gtgttgttcc agtaacggtt gatgccgttc cacccagctc atcagcattt gtttgcagtg
    32401 atcgtgtgcc accagcgcaa tatgtttccg cgaaggtaaa gtgcgagtcg tcagttccat
    32461 aatgtacatc cgtagttaac tttcctacag attactgtaa gtacttatcg ctgcaagata
    32521 aagactgaaa aagcctgcgc acaggcacaa aaatctcagg aggatggttg tttttccgcc
    32581 cattgcagga aagtatttcg cgtttgtggg tcagccagtt taaaccaata cttcagccgt
    32641 tgttctgtga gcacctgaga ctgcggtgga atagcatcca gctcggaaac acccgaaagc
    32701 agcgtactgt catctgccat catcgtggca aattgcggta gtgaagcgcg tttcgcggat
    32761 ttgttatagc gttcaacctc gacctcataa tcaatcgttt ttgccgttga ggtaatggcg
    32821 agaatatcca gctttaccgg aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc
    32881 gcggcatcaa aatggttagc ttcccgctca ttctccaggc gaggcaggcg aaaattgacc
    32941 tggttgatga gctgggtatt aaaactaacc accagtggag gggagatata gagccgttct
    33001 tcactattgg aaagatgaat cgtcttctca acgcgaaaca ctaactgatg cggtccattg
    33061 tccagttcaa tgctgtccgc accgcgcagc agagagctgg aaaccttctt accgtcgagt
    33121 accagaagat cgacatcagt agaaagccgc agcgtggtgg caaaaacgga taccggcaaa
    33181 cataatgcaa tcaaggtggt cacgatgccg gttttcatag atagctcctg ttaaaagacc
    33241 gcccgaataa taatgtgtca aaatttttct ttaatcatac ttactaacat atagacatat
    33301 ttcccgcttt tgctctcatt cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg
    33361 tacactttgt taaacagtct ggaggaaata atgaaagaaa ccgatattgc aggcatttta
    33421 acgtccactc acaccattgc gctggtgggg gcaagcgaca aacccgatcg tcccagctat
    33481 cgcgtgatga aatatctact cgaccagggg tatcacgtca ttcctgtttc gccaaaagtt
    33541 gccggcaaaa cactgctggg ccagcagggg tatggcacgc tggcagacgt tccggaaaaa
    33601 gtcgatatgg tcgatgtttt ccgtaattca gaagcggcgt ggggcgtggc gcaggaagct
    33661 atcgccattg gcgcgaaaac cctctggatg caactggggg tgattaatga gcaagcggca
    33721 gtactggcac gggatgccgg gttaaatgtg gtgatggatc gctgcccggc tattgagatc
    33781 cctcgcctgg gcctggcaaa ataaaaaata cccggaaggc gaaaaccttc cgggtatgtt
    33841 tatgagaatt agttacgcag acgcggcgcc tggagttgtt tgcggatggt ctgcgccagc
    33901 tcatccatgg atggctgttc aggatgttca tcctgcaatt cgctacttag ctgggcttca
    33961 gccaggtagg tatgaaccgg taggccgtta tcatcctcca tcaccacgtg ataccacgga
    34021 gcggcgcgaa gctcgtcatt caccgccagc tcatcaggcg acggttcaga gagcgagtaa
    34081 accgggtcga tatccacgac cactccgaga taacctaaca gggaatggcg gacctgctgg
    34141 ccgataccga atttgctggc aatcatagtc acctcccggg aaatctttac ctttgatata
    34201 agggtaaaat tccacatttc aagttacatg acgcgacagg caaacccttt cagatatagc
    34261 ccttccgggt aggtagcgat caccggatga tcggcggcct gacggaactg ctctataaat
    34321 tgtacatcac gaccggcatc aattgcggca tccgcgatga ttttctgaaa taaatcgctg
    34381 gtcatcagac cggaacagga gaaagtcagg agaacaccgc cttcattcag caactgaatc
    34441 gccagcatgt tgatgtcttt atagccacgg cacgcgccca tcaactggct tttattctca
    34501 acaaacttcg gcgggtccat cacgataaca tcaaattttt caccgcgatc gcgataagta
    34561 cgcagcaatt taaagacatc atcacggaca aactctgcct tgctcagatc cagtttgttc
    34621 agctcaacgt tctgccgtgc aatatccagc gcttcctggg aggtatcaac gctgacaacc
    34681 tggctgcaac cgcccatcaa tgccgatacg gcgaaaccac cggtatagga gaaacagtta
    34741 agtacgcgtt tattttcgac gtagcggcgg gtagccaggc ggctgtcacg ctggtccagg
    34801 tagtagcccg ttttgtgccc gtgctgaata tccaccagca gtttcatgcc gtgttcttca
    34861 atcggcagca gggcaggcgg caactcgccg gtgatggggc cctgagtcag ctccatccct
    34921 tcttttttac gtaccgcgac gtcgctgcga tcgtaaatgg cacattccgg gtacaacgtt
    34981 tgcagggcgc taattaatgc cgcgcgctga tactctgcgc cagcactcag caattgcagc
    35041 accagaaaat taccgaaacg atcgatagta atacccggca ggccatcaga ttcaccggcg
    35101 attaaacgat agctgtcgag gccatctttt tgcgccagcc agtcacgcca tttttgtgct
    35161 tgctgcaaac ggcgggtaaa aaaagcaatg tcgatagact cagacgggtc aaacgtccag
    35221 acgcgcgccc ggatttgcga agctggcgaa taagcgccgc gtgctaacca ttttccctga
    35281 tgatcaacaa tatcgatggt ttcaccgagg ctggctttac cttccatgcg ggcaacggcc
    35341 ccggaaaaga cccacggatg gcgacgaagt aatgattttt cgcgcccttt ggctaacact
    35401 aaacgtacac tcataattta cttttctgtc gatgcaaaga aatgggcgtc attgtccgga
    35461 gttttccatg gatttgcaac gcgtccatta aggataagga gagcagcaat gtcgaaagtc
    35521 tgcataattg cctgggttta cgggcgagtt cagggcgtag gattccgcta caccacgcag
    35581 tacgaagcga aaaaactggg actaaccggg tacgccaaaa atcttgacga tggcagcgtg
    35641 gaagtggttg cctgcggtga agaagggcag gtggaaaaat taatgcagtg gttaaaaagt
    35701 ggcggcccgc gttctgcgcg tgtggaacgg gtgctcagcg agccgcatca tccctccggg
    35761 gagttaacgg attttcgtat ccgttaaatg cattttaccg gtttaggcag gccagcaatt
    35821 ttggtggctt gctttgccgg acctttcggg aacagtcggt acagatagcg gctattgcct
    35881 ttctcttcgc caaatttatt cgccatcgct tttaccagca tacgaatcgc cggagaagta
    35941 ttgaattcca gatagaaatc acgcacaaaa cgcaccactt cccagtgttc tggcgacaga
    36001 gtaatccctt cgttttctgc aatcaccacc gccagcggct cactccactg gctgctttct
    36061 ttgagatagc cttcggtatc cgtttctatc tctttacctt cgaagatcag cataattctt
    36121 tactacctta atcagactgg cggcagtgta acaaaaaaca aagccccgca taaagcgagg
    36181 ctcggtaagt aaccctgggc gagattaatc gcggctggcg aagcccagaa tgctcagcag
    36241 gctgacgaag atgttgtaca gcgaaacata caggctaacc gtggcacgaa tatagttcgt
    36301 ttcaccgcca tgaatgatgt tgctggtttc aaacaagata gcgccagagg agatcagaat
    36361 gaagaccgcg ctgatcgcca gatgcagagc aggcagttgc aggaagatat tagcaaccat
    36421 accaatcagc accaccacaa tacccgccat cagcataccg ccgaggaaag acatatcttt
    36481 gcgggtggtc agcacatacg cagagcagca gaagaacact aacgccgttc cgcccagtgc
    36541 catagcgatt acgtcaccca ttccggcaga cagataggtg ttcagaattg gtccgaggat
    36601 ataacccaga aaaccggtaa aggcgaatgc ggagataatc ccggtcggct tattcgccgt
    36661 tttgtaggtc aggaacatca aaccatacat acccaccagc gtcagaatca gacccggaga
    36721 tggcagcatc agcaccgtac tggcagttgc ggtaatcgcc gaaaaggcca gcgtcaggct
    36781 cagcagaaaa taggtattac gcagcacctt atgggtgcta agcagcgatg tacggtcatg
    36841 tgaagaacta actatacgat ccattgagtc actctctatg acagatgtaa ttaattaagc
    36901 agcataatga taatgcgtaa gggcacccag aagttttacc catctttacg catttgatct
    36961 ggaacaggtt taacagcgga ttatcaggtc attaggcagt gatataacgc cctgagaatt
    37021 tcgacaggca aaagaaaaag gggttagcat ttagctaacc ccttatctta tttggcggaa
    37081 gcgcagagat tcgaactctg gaaccctttc gggtcgccgg ttttcaagac cggtgccttc
    37141 aaccgctcgg ccacacttcc ggaatgacgc gcactataaa catcccgatg cggcgtgtaa
    37201 acccctaatt tgtttgtttg cctgaaaaat agccaaaagt gcattgatag cgtgaaacaa
    37261 cagcagattg atcatttcat caccatgaat tccttctcta tttactcgtt tagcaaccgg
    37321 ctaaacatcc ccaccacccg gcaaaaagaa aaataggtcc atttttatcg ctaaaagata
    37381 aatccacaca gtttgtattg ttttgtgcaa aagtttcact acgctttatt aacaatactt
    37441 tctggcgacg tgcgccagtg cagaaggatg agctttcgtt ttcagcatct cacgtgaagc
    37501 gatggtttgc cttgctacag ggacgtcgct tgccgaccat gtgcgcccgg tgttctgccg
    37561 gtgtcgcaag gagaagagac gtgcgatatg aataacgagg aaacatttta ccaggccatg
    37621 cggcgtcagg gcgttacccg gcgcagcttt ctcaaatatt gtagtctggc tgccacgtcg
    37681 ctgggattag gcgcgggaat ggcaccaaag attgcctggg cgctggagaa taaaccgcgc
    37741 attccggtgg tatggatcca cggcctggaa tgcacctgct gtaccgaatc ttttatccgc
    37801 tccgctcacc cactggcgaa ggacgtcatc ctttccctga tttccctcga ttacgacgat
    37861 actttgatgg ctgccgccgg aacccaggcg gaagaagtct ttgaagacat catcgcgcaa
    37921 tacaatggca aatatatcct cgcagtagaa ggtaatccgc cgctgggcga gcaggggatg
    37981 ttctgtatca gcagcggacg accgtttatt gagaaactca agcgtgccgc cgctggagcc
    38041 agtgcgatta tcgcctgggg aacctgcgcg tcctggggct gcgttcaggc cgcgcgacct
    38101 aatccgacgc aggcaacgcc tatcgacaaa gtcatcaccg acaaacccat tatcaaagta
    38161 cctggctgcc cgccgatccc ggatgtgatg agcgccatca ttacttacat ggtgacgttt
    38221 gatcgtctgc cggatgtcga cagaatgggc cgtccactga tgttttatgg tcagcgtatc
    38281 cacgataaat gctatcgccg cgctcacttc gacgccggag agttcgtcca gagttgggat
    38341 gatgacgctg cccgcaaagg ttactgcctg tacaaaatgg gctgcaaagg gcctaccacc
    38401 tataacgcct gttcctccac acgctggaac gatggcgttt ccttcccgat ccagtcaggt
    38461 cacggctgcc tgggttgtgc ggaaaatggt ttctgggatc gcggttcgtt ctacagccgc
    38521 gtggtcgata ttccacaaat gggtacccat tccaccgccg acaccgtcgg tttaaccgcg
    38581 cttggcgtgg tggcagcggc tgttggtgtg cacgcagtcg ccagcgccgt tgaccagcgc
    38641 agacgtcata accagcaacc tacagaaacc gaacatcagc caggcaatga ggataaacag
    38701 gcatgagcac tcagtacgaa actcagggat acaccatcaa caatgccgga cgccgcctgg
    38761 tggtcgaccc gattacgcgc attgaaggcc acatgcgctg cgaagtgaat attaacgatc
    38821 agaatgtgat caccaatgcc gtctcctgcg gcaccatgtt tcgcgggctg gagatcattc
    38881 tgcaagggcg tgacccgcgc gatgcgtggg cgttcgttga acgtatctgc ggcgtctgta
    38941 ctggcgtaca cgccctggct tcggtttacg ccatcgaaga tgccatcggt attaaagtgc
    39001 cggacaacgc caatatcatc cgcaacatta tgctggcaac gctctggtgc cacgatcatc
    39061 tggtgcactt ctatcagctt gccgggatgg attggatcga tgtgttagat gcgctgaaag
    39121 ccgacccgcg gaaaacctcc gaactggcgc aaagcctctc ctcatggccg aaatcatccc
    39181 ctggctattt cttcgacgta caaaaccgcc tgaagaaatt cgttgaaggc ggccagttgg
    39241 ggatcttccg caatggctac tgggggcacc cgcagtacaa actgccgcca gaagccaacc
    39301 tgatgggctt tgcccactat ctcgaagctc tcgatttcca gcgtgaaatt gtcaaaatcc
    39361 acgcggtctt tggcggtaaa aacccgcatc caaactggat tgtcggtggg atgccttgcg
    39421 ccattaacat tgacgaaagc ggcgcggtcg gggcagtcaa tatggaacgc ctgaacctgg
    39481 tgcagttgat tatcacccgc acggcggatt tcattaacaa cgtgatgatc cccgacgcct
    39541 tagccatcgg tcagttcaac aaaccgtgga gcgaaatcgg cacggggctt tctgataaat
    39601 gcgtcctcag ttacggcgcg ttccctgata tcgccaacga ctttggcgag aaaagtctgc
    39661 tgatgcctgg cggcgcggtg attaacggcg acttcaacaa tgtactgcca gtggatttag
    39721 ttgatccgca gcaggtgcag gagtttgttg accatgcctg gtatcgttat cccaacgatc
    39781 aggttgggcg tcatccgttc gatggcatta ccgacccgtg gtacaacccc ggcgatgtca
    39841 aaggcagcga taccaacatt cagcagctga atgaacagga acgctactcg tggatcaaag
    39901 cgccacgctg gcgcggtaac gcgatggaag tggggccgct ggcacgcacg ttaatcgctt
    39961 atcacaaagg cgatgctgcg accgttgagt cggtcgatcg tatgatgtcg gcgttgaaac
    40021 tgccgctttc cggcatccag tcaacgttgg gccgcatttt gtgtcgcgcg cacgaagcgc
    40081 agtgggccgc aggtaagttg cagtatttct tcgataaact gatgaccaac ctgaaaaacg
    40141 gcaatcttgc caccgcttcc acggaaaaat gggaaccagc aacctggccg acagagtgcc
    40201 gtggtgtcgg ttttaccgaa gcgccgcgcg gggcgttagg ccactgggct gccattcgcg
    40261 atggcaagat tgatctctat cagtgcgtgg tgccgaccac ctggaacgcc agcccgcgcg
    40321 atcccaaagg gcagattggc gcttatgaag cggcgctgat gaacaccaaa atggcgatcc
    40381 ccgagcaacc gctggagatc ctgcgtactc tgcacagctt tgacccgtgc ctcgcctgtt
    40441 caacacacgt gctgggcgac gacggtagcg agcttatctc cgtgcaggtg cgttaacagc
    40501 gaaggagaat catcatgcaa cagaaaagcg acaacgttgt cagtcactat gtctttgaag
    40561 cacccgtgcg gatatggcac tggctgacag tgttatgcat ggcggtgttg atggtcaccg
    40621 gttactttat cggcaagccg ctaccttccg tcagcggcga ggcgacatat ctgttctata
    40681 tgggctacat caggttaatt cacttcagcg ccgggatgat ttttaccgtg gttttgctga
    40741 tgcggatcta ctgggctttt gttggcaatc gttactcccg cgagctgttt atcgtgccgg
    40801 tatggcgtaa aagctggtgg cagggcgtgt ggtatgaaat ccgctggtat ctgtttctgg
    40861 caaaacgtcc gagtgccgat ataggccata accccatcgc ccaggcggcg atgttcggct
    40921 atttcctgat gtcagttttt atgatcatca ccggctttgc gctgtacagc gaacacagcc
    40981 agtacgctat ttttgcgccg ttccgttatg tggtggaatt tttctactgg acgggcggca
    41041 actcaatgga cattcacagc tggcatcggc tggggatgtg gctgattggc gcatttgtga
    41101 tcggccatgt ctacatggcg ctgcgtgaag acatcatgtc cgacgacacg gtgatctcca
    41161 ctatggtcaa cggctaccgt agccacaaat ttggcaaaat aagtaacaag gagcgttcat
    41221 gagcgagcaa cgcgtggtgg taatggggct gggcaacctg ctgtgggccg atgaaggctt
    41281 cggcgtgcgg gtggcggaac ggctgtatgc ccattaccac tggcccgagg atgtggagat
    41341 tgtcgatggc ggcacccaag gactgaactt gctggggtat gtcgaaagcg ccagtcatct
    41401 gttgattctc gatgccattg attacgggct ggaacccgga acgctacgaa cgtatgccgg
    41461 agaacgcatt ccggcttatc tcagcgcgaa gaaaatgagc ctgcaccaga acagtttctc
    41521 cgaagtgctg gcgctggcag atatccgcgg acatctgcca gcacatattg ccctcgtcgg
    41581 tctgcaaccc gcaatgctcg acgactacgg cggcagcctg agcgaactgg cgcgggagca
    41641 actgcccgcc gcggaacagg cggcgctggc gcagctggct gcgtggggaa ttgtgccgca
    41701 accggctaat gaatcgcgct gtctcaatta tgactgtctg tcgatggaaa attacgaagg
    41761 cattcgtttg cgccagtacc ggatgacact ggaggagcag ggatgagcaa cgacacgcca
    41821 tttgatgcgt tgtggcaacg aatgctggcg cgcggctgga cgccgatcag tgaatcccgt
    41881 cttgacgact ggcttacgca agcgccagac ggcgtggtgt tattaagcag cgatccgaaa
    41941 cgcacgccag aggtcagcga taatccggta atgattggcg aattattgcg cgagtttccc
    42001 gattatacat ggcaggtagc gattgctgac cttgagcaga gcgaagccat cggcgatcgc
    42061 tttggcgtct ttcgctttcc cgccacatta gtatttacct gcggaaacta tcgcggcgtg
    42121 ctgaatggta ttcacccgtg ggcggaactg ataaacctga tgcgcggact tgtcgaaccg
    42181 cagcaggagc gtgcctcatg agcgaaactt ttttccatct gctggggcca ggaacgcaac
    42241 tgaacgatga cagtttcagc atgaatccac tgccgatcac ctgtcaggtg aatgatgaac
    42301 cgagtatggc ggccctggag caatgtgctc acagcccgca ggtgattgcg ctgttaaacg
    42361 agttacaaca tcaactaagc gaacgccagc cgccgttggg cgaggtactg gcagtcgatc
    42421 tgttaaatct caacgccgac gatcgtcact ttatcaatac gcttctcggg gaaggggaag
    42481 tgtcagtgcg cattcagcag gctgatggta gtgaaagtga aattcaggag gcgatcttct
    42541 gcggattatg gcgggtacgc agacgtcacg gcgaacagtt gctggaagac aaactggagg
    42601 ctggctgcgc accgctggcg ttgtggcagg cggcaacgca aaacgtcttg ccgacagatt
    42661 cgctgttacc gccgcccatt gatggcctga tgaatggcct accgttggcc catgaattac
    42721 tggcacatgt acgtaacccc gacgcacagc cgcacagcat taatctgacg caattaccca
    42781 tcagcgaagc tgaccggctt tttctctcac gtctctgtgg gccgggaaat attcagattc
    42841 gtaccattgg ctatggcgag agctatatca acgccacggg gttacgccat gtctggcatt
    42901 tacgctgtac ggacacctta aaaggcccgt tactggaaag ttatgaaatc tgcccaatac
    42961 cggaagtggt gctggcagcg ccagaagatt tggtcgactc tgcgcagcgg cttagcgagg
    43021 tatgccagtg gctggcggaa ggtgcaccga cgtaaaaaga cggtaagtat cgctttcagg
    43081 cttatgaata tcgcaatcgg cgaatacctc tggtcgtaga gtttcaggat aaagagggag
    43141 atctaccatt aacgggttat ttttctctct tcgcctacag gagtgcgcat gtgggatgtc
    43201 attgatttat cgcgctggca gtttgctctg accgcgctgt atcacttttt atttgtaccc
    43261 cttaccctgg ggctgatttt tttgctggct atcatggaaa ccatttacgt ggtcaccggc
    43321 aaaacaatct accgcgatat gacgcgcttc tggggtaagc tcttcggtat caactttgct
    43381 cttggcgtgg ctaccggcct gacgatggag tttcagtttg gtactaactg gtcattctat
    43441 tccaactatg tgggcgatat ttttggcgca ccactggcga tggaagcatt aatggccttc
    43501 ttcctcgaat ccacctttgt cgggctgttc ttcttcggct ggcaacggct gaataaatac
    43561 cagcacctgc tggtgacgtg gctggtggcg ttcggttcaa atctctctgc gttgtggata
    43621 ttgaatgcca acggttggat gcaatacccg actggtgcgc attttgatat cgacaccctg
    43681 cgtatggaga tgaccagttt cagcgagctg gtctttaacc cggtcagcca ggtgaaattt
    43741 gtgcacaccg taatggcggg atacgtgacc ggggctatgt ttattatggc gatcagcgcc
    43801 tggtatttac tgcgcggacg ggagcgcgat gtcgcattac gctcgtttgc catcggttcc
    43861 gtcttcggta ctctggcgat tatcggtacg ctgcaactcg gagacagttc tgcgtatgaa
    43921 gtcgcgcaag tacaaccggt aaaactggcg gcgatggaag gggagtggca aacagaacct
    43981 gcacctgcac cgttccatgt ggttgcctgg ccggaacagg atcaagagcg taacgccttt
    44041 gccatcaaaa ttcccgcgct gctagggatc ctcgccactc actcattaga taaacccgtg
    44101 ccgggtctga agaatttgat ggctgaaacc taccctcgct tgcaacgtgg acgtatggcc
    44161 tggctgttaa tgcaggaaat atcgcaaggc aatcgtgagc cgcatgtgtt gcaggcattc
    44221 cgggagctgg aaggcgacct gggttacggc atgttgctct cccgctatgc gccggatatg
    44281 aatcatgtca cagccgcaca gtaccaggcg gcgatgcgtg gcgcgatacc tcaggttgcg
    44341 ccggtattct ggagtttccg catcatggtg ggctgtggtt ccctgctgct actggtgatg
    44401 ctgattgcgc ttgtccagac gctgcgtggc aaaatcgacc agcatcgctg ggtgctgaaa
    44461 atggcgctct ggagtttgcc gctgccgtgg attgcgattg aagccggatg gtttatgacc
    44521 gagtttggtc gtcagccgtg ggcgatacag gacatcttac cgacatactc cgcgcactcc
    44581 gccttaacca caggacaact ggctttctca ctgatcatga tcgtagggct ttacaccctg
    44641 ttcttaatcg ccgaagtcta cctgatgcag aaatatgccc gtctggggcc gagcgcgatg
    44701 cagagtgaac aaccgacgca gcaacagggg taaaggagaa aatcatgttt gattatgaaa
    44761 cattgcgttt catctggtgg ctgctgattg gcgtgatcct ggtggtcttt atgatctccg
    44821 acggatttga catggggatc ggctgtctgc tgccgctggt ggcgcgtaat gatgatgaac
    44881 gtcggatagt gataaacagc gttggcgcgc actgggaagg caatcaggtc tggttgatcc
    44941 tcgcaggtgg ggcattattt gccgcctggc ccagagtgta tgcagcggcg ttttccggct
    45001 tttatgtggc gatgattctg gtgctgtgct cgctgttctt ccgcccgctg gcctttgatt
    45061 atcgcggaaa aatcgccgat gcacgctggc gtaaaatgtg ggatgccgga ctggtcatcg
    45121 gcagtctggt gccgccggta gtcttcggta ttgcattcgg caacttgctg ctcggcgtgc
    45181 cgtttgcttt cacaccgcaa ttacgcgtgg aatatctcgg cagcttctgg caactgctga
    45241 cgccattccc tttattgtgt ggattgctca gtcttgggat ggtgattttg caaggtggcg
    45301 tctggttaca actgaaaact gttggtgtga ttcatctgcg ttcacagctg gcgaccaaac
    45361 gcgccgcact actggtgatg ctgtgctttt tgctggcggg ttactggctg tgggttggta
    45421 ttgatggctt tgtgctgctc gcccaggatg ctaacggtcc ttccaatcca ttaatgaaac
    45481 tggtggcagt gctacctggt gcctggatga ataatttcgt cgaatcgccc gttttgtgga
    45541 tcttcccgct gctggggttc ttctgcccat tgctgacggt gatggcgatt tatcgtggtc
    45601 gcccgggttg gggatttttg atggcgtcat tgatgcaatt tggcgtgatt ttcacggcag
    45661 gcatcacgct gttccccttt gtcatgccgt caagcgtgag tccgatctcc agcctgacgt
    45721 tgtgggacag cacttccagt cagctgacgc tgagcattat gttggtaatc gtgctgatat
    45781 ttttgcccat tgtgttgctc tacactctct ggagctacta caaaatgtgg gggcgcatga
    45841 cgacagaaac tctccgccgt aacgaaaacg agctgtacta aggagcagga acaatgtggt
    45901 atttactttg gttcgtcggc attttgttga tgtgttcgct ctccaccctt gtgttggtat
    45961 ggctggaccc gcgtctgaaa agttaacgaa ggtaagcctg atgaggcgca ttagcgtcgc
    46021 atcaggcaat caataatgtc ggatatgaaa agcggaaaca tatcgatgaa agcgatcttg
    46081 atcccattct tatctcttct gattccgtta acaccgaaat ctgcattcgc tcagagtgag
    46141 ccggagctga agctggaaag tgtggtgatt gtcagtcgtc acggtgtgcg tgcaccaacc
    46201 aaggccacgc aactgatgca ggatgtcacc cctgacgcat ggccaacctg gccggtaaaa
    46261 ctgggttggc tgacaccgcg cggtggtgag ctaatcgctt atctcggaca ttaccaacgc
    46321 cagcgtctgg tggccgacgg attgctggca aaagagggct gcccgcagcc aggtcaggtc
    46381 gcgattattg ctgatgtcga cgaacgtacc cgtaaaacag gcgaagcctt cgccgccggg
    46441 ctggcacctg actgtgcaat aaccgtacat acccaggcag atacgtccag tcccgatccg
    46501 ttatttaatc ctctaaaaac tggcgtttgc caactggata acgcgaacgt gactgacgcg
    46561 atcctcagta gggcaggagg gtcaattgct gactttaccg ggcatcggca aacggcgttt
    46621 cgcgaactgg aacgggtgct taatttcccg caatcaaact tgtgccttaa ccgtgagaaa
    46681 caggacgaaa gctgttcatt aacgcaggca ttaccatcgg aactcaaggt gagcgccgac
    46741 aatgtctcat taaccggtgc ggtaagcctc gcatcaatgc tgacggagat atttctcctg
    46801 caacaagcac agggaatgcc ggagccgggg tggggaagga tcaccgattc acaccagtgg
    46861 aacaccttgc taagtctaca taacgcgcag ttttatttgc tgcaacgcac accagaggtt
    46921 gcccgcagtc gcgccacccc gttattggat ttgatcatga cagcgttggc gccccatcca
    46981 ccgcaaaaac aggtgtatgg tgtgacatta cccacttcag tgctgtttat cgccggacac
    47041 gatactaatc tggcaaatct cggcggcgca ctggagctca actggacgct tccaggtcag
    47101 ccggataaca cgccgccagg gggtgaactg gtgtttgaac gctggcgtcg gctcagcgat
    47161 aacagccagt ggattcaggt ttcgctggtc ttccagactt tacagcagat gcgtgataaa
    47221 acgccgctgt cattaaatac gccgcccgga gaggtgaaac tgaccctggc aggatgtgaa
    47281 gagcgaaatg cgcagggcat gtgttcgttg gccggtttta cgcaaatcgt gaatgaagta
    47341 cgcataccag cgtgcgctct ccatcaagac aaataaacat ttgccgctgt tggtccacgc
    47401 aggccattta cgcggcaaaa ttccacgcgt aatcctggta taagcacttc tgcgtcgcgg
    47461 ggagtgaatg cggaaatatg gacctggact tctttacgac cgtcggaggg gataatgaat
    47521 cctttgccgc ttttgcgatc aaaggttttg acaattcctg tcattttacg ggacaaacaa
    47581 attccttact gaaaatactg cgctgcacta tacggggtta ataaaataaa gccagcgata
    47641 tttaagaccg ccggacggct aaaataaaat ttgcttaatc tcaattatca tgcgttaata
    47701 gctgcgtcgg tttgaaagac agacagcata caaagtagtt tactaaagca gttctcatta
    47761 tcaggcatta tccccttctt ttgagtctct ctcctgaaca ctaagtagtt tctgtattaa
    47821 agccctgttt gccgaaaggc ccaaaatgaa ggaagtaaaa tatgtctaat aaaatgactg
    47881 gtttagtaaa atggtttaac gcagataaag gttttggctt tatcactcct gatgatggca
    47941 gcaaagacgt tttcgtccat ttcactgcca tccagagcaa tgaattccgc acgctgaacg
    48001 aaaatcagaa agttgaattt tctattgagc aggggcaacg tggtcccgcg gcagcgaacg
    48061 ttgttacgct ctaaggttgc cgttattact caacatctcc atttccgctg tccatgttgc
    48121 catggttcac agtaccgcac atcggcattc gatgtgacgg agcgaaaccc tttgggtgca
    48181 aagtgcattt tttgtaaatc gacgatgatc acctttgata acgttgcgct gcaaatacgc
    48241 actgaccatg cgccgctgga tttcacaaaa taatatcagg ctccctcgtg gagccttttt
    48301 tatatctgcc ttatttttct tcaacgctgt atgtatagta agcgataacc tgttgattat
    48361 tgaatctttc ggggagatgg cttataacat ttcttacctg accagggtac cgggaaccaa
    48421 caccttactg gcgtgttgct gtcttttaag accagaagag gttaacagtg aatattgaag
    48481 agttaaaaaa acaagccgaa acggaaatcg ccgactttat cgcgcaaaaa atcgccgagc
    48541 tgaacaagaa tacagggaaa gaagtctctg aaattcgctt caccgcacga gaaaaaatga
    48601 ccgggcttga aagttatgat gtcaaaatca aaataatgtg attttgtgaa catcaccccg
    48661 tgcgaggtga tgttccgctt gttgctaatt tagtgaccaa tcattggcga ttgcggaatt
    48721 aagcgttggt acaattcctc cggcaccggg ctttgccata ctcccgcata cattgcgtaa
    48781 ccaatcgccg caaacataat ccccataacc agtagcgtca ttaaccagcc agacaacgca
    48841 aaggcttttt tatttgccgc aggtttttgc agtgaaaagg ccaatgttga ggctaccgga
    48901 catgactcta cgcaagtcat acagccggta cattccactg ttcgtacctg aattaattta
    48961 tcgaccggga tccgtgatgg gcaacttttt gcgcatttgc cacaatcgat acaactttcg
    49021 gcattgcgac gaatcttaaa cggcgataat agcgaaacca cgcccatcag cgcgccatat
    49081 gggcaaagat aacgacacca ggcatggcga ataaacaggc tggcaatcag caaaacgatc
    49141 atgctgatta atgtcgcggt ccccatatga cgaaagaaat cgagcatttt aacgtccatc
    49201 accacgctgt agggcgacaa cataaaatag tgaatcgcct gagcgggcat caataacgcg
    49261 atatagagaa aaaaactcaa cagcaaatac ttcacgccgc gcagaggaat atccagccag
    49321 cggggaagga caaattgccg accaaacagt ttgttaccga gatcgccgat taattccgaa
    49381 agcgtaccaa ccgggcataa ccatgaacaa aaggcctttt tgagtaatag actgatgacg
    49441 ataaaagcga ccaataacag catcgcggcg gcgtggacgg acggtaactg acctgttgca
    49501 aggctatatt tcagattcat cagaccggca atcggtagcc agccttcgat accacccggt
    49561 ctggtgacaa atgtcgtact acctgccgtt tcgtaatagc gcacccaata ccagaacgtg
    49621 atggcaatat aaatattaat tgccaacagt aataattgcg tcgctttacg ccaggtcgtg
    49681 gcattgcgcc agtcattcca cggtaatttg ccgcccgtcg tgcctggccg ccgttgccag
    49741 cgggttcttt ttttctctgc catgattttg ccagtccgtt aagttgcata ccaaatgcca
    49801 ctattctagt tgttcttaac tggctgatat tgattcaaat cgcgttcagg tctttcttat
    49861 taaccatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa ggggggcaga
    49921 taatggctgc tgctccagtt gtgcgcattg ctggctggcg ctacgcattc ccagactcga
    49981 gcaactgctt tttagctgat gtgcggcacg ctttattttc tcgttatcct gactggcgcg
    50041 ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataata tcagccattc
    50101 gtggatcttc tccgccccca ttaaatgagc atcttcattg agttgcgata catccagtgg
    50161 ttgatcgtta ttgacttgca gttggagata gtgcgccagt aattgaccga gcacttcacg
    50221 cggcaccggt ttagggataa tcccgcgaaa tagcgaactg gtacgctgtc gcagtgtttc
    50281 gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct gtgccagttg
    50341 tcgggcaagc gtaatgccgt cgatatccgg tagatcaaaa tccaccagtg cggcagcaaa
    50401 cggttcacta ttttgcagtg tctctaaagc ctgcgcggca tttcctacgg caacgacctg
    50461 cgccccactg ctgttcaaca tctcaacggt aatccgctgg gttagcgggt tatcttcaat
    50521 taacagcaaa cgtaaaccgt ccagacgcac cgcctgattg actgtttttg gcacgggtgc
    50581 agtggcaaca cgtaacggca cgcgtaaaca aaaacagctt ccaacctccg gcgtgctggt
    50641 ggcgctcaat tcgccgccca tcgcctgggc cagacggcta ctgatagtca gccccagccc
    50701 ggtgccgccg cgtttgccgc taacctgaac aaatggctgg aagatttcag ccagtttcgc
    50761 gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgttcgc catcaatgcg
    50821 actacgcaga ataatgtgcc cttcgtcggt gaaacgcagg gcgttgctca acaggttggt
    50881 aatgacctga cgaatacgcc gcggatcgcc cattaacgca gtcggtacat cgtcggcaat
    50941 ttctgttgcc aggcgaatcg ggcgaccttt cacccttccg ctcattaatt gcagggtact
    51001 ttccagcagc gggcgcggtt caaagggttc atcgctgacc gaaacattct tgccgcctgc
    51061 ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt caccagagtc
    51121 ggtaatcgcc cgcaaatcat cgcgctgggc gttaagtgcg gggttatctg ccagcagctg
    51181 tgcggtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg ccgccagaaa
    51241 cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt gttctatcac
    51301 cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt cgcggtggcg
    51361 attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg tatccagttc
    51421 ccgtacgcca gcggtttccg ggaaagggga gtcgatatca ccatccagca agcgttgcag
    51481 cgcctgcgtt tgttctgcaa gagggcgcgt gactgagcga taaaccacgc gccagaggat
    51541 cagaatcagt gcgcaaagag aaactaatcc cagcaataac aggctgtatt gcccgcgtgc
    51601 actggctttt tccagatgag ccagcccgtg ctgattacgc agctcaatgg tgtcgaccag
    51661 ctgactgact tcgctactaa actgcgcgaa ttgggcgatg ttattttggg cgagagtttg
    51721 caggtgattg ctgatttcac tgtcctgctg atacagcgcc agcaaatcgc tatattggct
    51781 aacggtagtt aacgttgttg ctacctgagt acgaacaccc ggatcttcaa tgcgtatttg
    51841 ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg gcgcattttt
    51901 ctggatctgt tccagcccca gattcatcac catttgctgc acccgcaggg cgctaaggcg
    51961 cagttcattc atctggttaa catactcaag atcgatatca atcagccgat cgagtgcact
    52021 ttctgcggct tgacgctgat gttgttcgat caaatcgtaa atcccggcct gggtcgctcc
    52081 ggcggaggtt gccgcattat tcgcctgacc ttgcgccagg cgtgcgattt catcggcggc
    52141 ggcgactatc tgctgactga gttgctgttg ttgctggcga agttgcaaac gctgccccac
    52201 cagttcccct tgctgacgta acgaacggga gatctcctgc tcctgttgtt caatagcggt
    52261 ggtgtcaaaa ccttgttccc gtaacgcttg cagcaacgca ttaatcttca gactttgtgc
    52321 ggtgagcatt cgcccctgcg cctgccacat cttttcgtta tcggcactgg tcaggttctg
    52381 cgcggcgaaa agttcccagg cgctggcttc gctcaactgg cgcgccatat tcatggtagg
    52441 aatcaatgcc tgagtgttgt ctttttccac ctggctgata aagcgcaggt tgtaccatcc
    52501 caccagggta ctggtcaggg ttaacagcgc catcagggca aagcccatcc agagtcttcg
    52561 ggtcagggtt aaattcacgg tcggtgcact taaggtgaaa aaggttgagt cgcaaagcgg
    52621 aatgcatcta gcataaagcc ttattattga tgaggctatc atgcgcgtac tgctattttt
    52681 acttctttcc cttttcatgt tatcggcatt ttcggctgat aacctgttgc gctggcatga
    52741 tgcgcagcat ttcacggtgc aagcctctac gccgcttaaa gccaaacgcg catggaaact
    52801 gtgcgcgctt tatcccagcc tgaaagattc atactggtta tcgttgaact atggtatgca
    52861 ggaggctgct cgccgctacg gtgtggattt aaaagtgctg gaggcaggcg gctacagcca
    52921 gttggctacc cagcaagcac aaatcgacca gtgtaaacag tggggcgcag aggccatttt
    52981 gctcggtagt agcacgacgt catttcccga tctgcaaaag caggtagcaa atctgccggt
    53041 gatcgaactg gtaaatgcta ttgatgctcc ccacgtgaaa agccgcgttg gtgtgccctg
    53101 gtttcagatg ggctatcaac cagggcgata tctggtgcaa tgggcgcacg gtaaaccact
    53161 gaacgtgctg ttgatgcccg gccccgataa cgccgggggc agtaaggaga tggtagaggg
    53221 ttttcgcgca gccattgccg gaagcccggt gcgtattgtt gatattgcgc tcggtgataa
    53281 cgatattgaa atccagcgta acctgttgca ggagatgctg gaacgccatc cagaaatcga
    53341 cgtcgttgcc ggaacggcca ttgcggcaga ggcggcaatg ggggaagggc gtaacctgaa
    53401 aacgccgctt accgtggtgt cgttttatct ttcacatcag gtgtatcgcg ggttgaagcg
    53461 gggaagagtg attatggctg ccagcgatca aatggtctgg caaggggaac tggcggttga
    53521 gcaggccatc aggcaattac aggggcaatc ggtgtctgat aatgtcagcc caccgatttt
    53581 agtcatgacg ccgaaaaatg ccgaccgcga acatatccgc cgctcgctgt caccaggggg
    53641 atttcgtccg gtttattttt atcagcacac atcagcggct aagaaataac cttcgccatg
    53701 ttgtgtcacc agtaaatccg cactgagttt atggcgtaaa cgacgaatta acacatcgac
    53761 ggtgcgcagg tcagggtttt ccacccgacg cgcagagagc atacgcagca gacgttcacg
    53821 gctgagaatt tcgcccggat tagtcacaaa tgccaccagc atttcatact ctgcgcgggt
    53881 cagtttaatc ggctcgccat cccgctccag cgtatggcgc gacacattca ggcaataacc
    53941 ggcaaagcga tagcagttgt cctgagtgtg cggttgcgcc tgtcgcgcga ggtcgattcg
    54001 ccagagcaaa tttttcactc gtaccaccag ttcgcgcagc tccagcggtt tggtgacgta
    54061 atcgtctgcg cccatttcca gcccaacaat acggtcaatc cgatcgctgc gtccggtaac
    54121 cagaataatc cctaccgttg agcgttctcg cagggcgcgg gttaacatca ggccattttc
    54181 atcgggcaaa ttgatatcca gcagaatcaa atccaccggc tgattctgca taatttcccg
    54241 cagcccggca ccgctcgccg taacggaaac ggtatacccc tcctgagtga agtaagattg
    54301 taatcgcgcc tgggtaaccg gctcatcttc aacaataaca atgtgatgtg gcatcagagg
    54361 gttttactca ttctgttcat atctgttcat attctgccgt aagccgttca tcctgaccag
    54421 tgccgctgtt catatttgct cattaagatc gcttcactaa accataattt tacaggggct
    54481 attatgcgga aactctggaa cgcgctacgc cgacccagtg ctcgttggtc ggtactggcg
    54541 ctggtcgcta ctgggattgt gattggcatt gcgctgattg tattgccaca cgttgggatc
    54601 aaagtcacca gcacaaccga attttgtgtc agttgccaca gtatgcaacc ggtgtatgaa
    54661 gaatataaac agtcggtgca tttccagaac gcctccggcg tgcgagctga atgccatgac
    54721 tgtcatatcc cgccggatat tccaggcatg gtgaagcgca aactggaagc gagcaacgac
    54781 atttaccaga cctttattgc ccactccatt gatacacctg agaaatttga agccaaacgc
    54841 gcggaacttg ccgagcgtga atgggcgcga atgaaagaaa acaactcggc aacctgccgc
    54901 tcctgccata actacgatgc gatggatcat gcgaagcagc atcctgaagc ggcgcgtcag
    54961 atgaaggtgg cagcgaaaga taatcaatcc tgcatcgact gtcataaagg tattgcccac
    55021 cagttaccgg atatgagtag tggcttccgt aagcagttcg atgagctgcg cgccagtgct
    55081 aatgatagtg gtgacacgct gtactccatt gatattaagc cgatttatgc ggcgaaaggc
    55141 gataaagaag cgtctggttc tctgctgcct gcttcggaag tgaaagtcct taaacgtgac
    55201 ggcgactggc tgcaaattga aatcactggc tggacggaaa gtgcaggacg tcagcgtgta
    55261 ctcacccaat tcccaggtaa acgcatcttt gttgcctcga ttcgtggtga tgtgcagcag
    55321 caggtgaaaa cactggagaa aaccaccgtt gccgacacca ataccgagtg gagcaagttg
    55381 caggccactg cgtggatgaa gaaaggcgac atggtgaacg atatcaaacc gatctgggct
    55441 tatgcggatt cgctgtacaa cggcacctgt aaccagtgcc acggcgcacc ggaaatatcc
    55501 cactttgacg ctaacggttg gatcggcacg ctcaacggca tgattggctt taccagcctc
    55561 gataaacgtg aagaacgcac tttgttgaaa tatctgcaaa tgaatgcgtc tgacaccgca
    55621 ggtaaagctc acggcgataa gaaggaagaa aaataatgaa caataacgat ctctttcagg
    55681 catcacgtcg gcgttttctg gcacaactcg gcggcttaac cgtcgccggg atgctagggc
    55741 cgtcattgtt aacgccgcgc cgtgcgactg cggcgcaagc ggcgactgag gctgtcatct
    55801 cgaaagaggg cattcttacc gggtcgcact ggggggctat ccgcgcgacg gtgaaggatg
    55861 gtcgctttgt ggcagcaaaa ccgttcgaac tggataaata tccgtcgaaa atgatcgccg
    55921 gattgccgga tcatgtacac aacgcggcgc gtattcgtta cccgatggta cgcgtggact
    55981 ggctgcgtaa gcgccatctg agcgacacct cccagcgcgg tgataaccgt tttgtgcgcg
    56041 tgagctggga tgaagccctc gacatgttct atgaagaact ggaacgcgta cagaaaactc
    56101 acgggccgag tgccttgctg accgccagtg gttggcaatc gacggggatg ttccataacg
    56161 cttcggggat gctggcgaaa gctattgcct tgcatggtaa tagcgttagt acgggcggag
    56221 attactctac cggtgctgcg caggtgatcc tgccgcgcgt ggttggctca atggaagtgt
    56281 atgaacagca aacctcctgg ccgctggtat tgcagaacag caaaaccatt gtgctgtggg
    56341 gctccgattt gctgaaaaac cagcaagcga actggtggtg cccggatcac gatgtttatg
    56401 aatattacga gcagctaaaa gcgaaagtcg ccgccggtga aattgaggtc atcagcatcg
    56461 atccggttgt cacatccacc catgagtatc tggggcgcga gcatgtgaag cacattgcgg
    56521 ttaacccgca aactgacgtg ccgctgcaac tggcgctggc gcatacgctg tacagtgaaa
    56581 acctgtacga caaaaacttc ctcgctaact actgtgtagg ttttgagcag ttcctgccgt
    56641 atctgctggg tgagaaagac ggtcagccga aagatgccgc atgggctgaa aaactgaccg
    56701 gcattgatgc cgaaaccatt cgtgggctgg cacggcagat ggcggcgaac agaacgcaga
    56761 ttattgctgg ctggtgcgta cagcgtatgc agcacggtga acagtgggcg tggatgattg
    56821 tcgttctggc ggcgatgctg gggcaaattg gcctgccagg tggtggtttt ggttttggct
    56881 ggcactataa cggcgcaggc acgccggggc gtaaaggcgt tattttgagt ggtttctccg
    56941 gctctacgtc gattccgcct gttcacgaca acagtgacta caaaggctac agcagcacca
    57001 ttccgattgc ccgttttatc gatgcgatcc tcgaaccggg gaaagtgatc aactggaacg
    57061 gtaaatcggt aaaactgccg ccgctgaaaa tgtgtatttt tgccggaact aacccgttcc
    57121 atcgccatca gcagatcaac cgcattattg aaggctggcg caagctggaa acggttatcg
    57181 ccatagataa ccagtggacc tcaacctgcc gctttgccga tatcgtgctg cctgcgacca
    57241 cgcagtttga gcgtaacgat ctcgaccagt acggcaacca ctccaatcgt ggcatcatcg
    57301 ccatgaaaca ggtcgtgccg ccgcagttcg aggcgcgcaa cgacttcgat attttccgcg
    57361 agctgtgccg tcgctttaat cgcgaagaag cctttaccga agggctggac gaaatgggct
    57421 ggctgaaacg catctggcag gaaggtgtac aacagggcaa aggacgcggc gttcatctgc
    57481 cagcgtttga tgacttctgg aataacaaag agtatgtcga gtttgaccat ccgcagatgt
    57541 ttgttcgcca ccaggcattc cgcgaagatc cggatctcga accgctgggc acgccgagtg
    57601 gcctgattga gatctactcg aaaaccatcg ctgatatgaa ctacgacgat tgtcaggggc
    57661 atccgatgtg gtttgagaaa atcgaacgct cccacggtgg gcctggctcg cagaagtatc
    57721 cgttgcatct gcaatctgtg catccggatt tccgacttca ctcgcagtta tgtgagtcgg
    57781 aaactctgcg tcagcaatat accgtagcgg gtaaagagcc agtatttatt agcccgcagg
    57841 atgccagcgc gcgcggtatt cgtcacggtg atgtggtacg cgtctttaac gttcgtggtc
    57901 aggtgttggc tggagcagtg gtttctgacc gttatgcacc cggcgtggcg cgaattcacg
    57961 aaggggcatg gtacgatccg gacaaaggcg gcgagccagg ggcgctgtgc aaatacggta
    58021 accccaacgt cttgaccatc gacatcggta cttcgcagct ggcgcaggcg accagtgcgc
    58081 acactacgct ggtggaaatt gagaagtaca acggaacagt ggagcaggtg acggcgttta
    58141 acggccccgt ggagatggtg gcgcagtgcg agtatgttcc cgcgtcgcag gtgaaatcat
    58201 gaccacgctg acagcacaac agattgcctg tgtttacgcc tggctggcgc agttgttctc
    58261 ccgtgaactg gacgatgaac aactgacgca aatcgccagt gcgcagatgg ctgaatggtt
    58321 ttcgttgctg aaaagcgaac cgccgctcac tgcggcggtg aatgggctgg aaaacagtat
    58381 tgccactctg acagtacgtg acgatgcccg tctggaactg gccgctgact tttgcggcct
    58441 gtttctgatg accgacaaac aagcggcgct gccgtatgca tcggcctaca aacaggacga
    58501 gcaagagatt aaacgcttgt tagttgaggc ggggatggaa accagcggca atttcaacga
    58561 accggcagat catctggcga tctatctcga tttgttgagt catctgcatt tttcgctggg
    58621 agaggggacc gttcctgcgc gaagaatcga cggtttgcgg caaaaaacac tgacggctct
    58681 gcgggaatgg ttaccagagt ttgctgcgcg ttgtcgacag tatgacagct ttggttttta
    58741 cgcggcacta agccagttat tgctggtgtt agtggagtgt gactaccaaa aaagataatg
    58801 ccgtttgtag gcctgataag acgcggcagg cgtcgcatca ggcattagtg cgcagttgcc
    58861 ggatgcggcg agaacgcctt atccggccta cgggaacagt gctttttgtg ggcctgataa
    58921 gatgcgttaa gcgtcgcatc aggcattatg gcgcagttgc cggatgcggc atgaacgcct
    58981 tatccggcct acgggaacag tgctctttgt aggcctgata agacgcgtta agcgtcgcat
    59041 caggcaacaa actcacgggt gagccacaaa ccgggaaagc cgctggcgca gcaggcggtt
    59101 ttcctgcttc aggtgcgcaa tatcatccat taacgtcagc gccaccgcga tccccggcca
    59161 gtccagagcc agttcatgac gcaggcgtac cgcgcgttgc accacaatgg cggcatggtc
    59221 gtcaaatacc caggttgtct cctggatctc actcggttca accaccccca aaccgacaat
    59281 ttcattcaac tcctcttcag agatgccggt atgcaggcaa aattcggtaa tagtaaaagt
    59341 caccgtaaca ttagccatta tgctttcccc caatctttac gtggatcaaa agacgactgg
    59401 gcgtctgcca gttgctgcca cagcgcggca gtgttttcat ccggtttcgg cggcatcacg
    59461 attttcagta ccgcatacag atcgccggtc tgttttttgc tcaccagacc tttgccttta
    59521 acgcgcaatc gttgcccggc ctggctgcca ggcgggatag tcagcaaaat gctttctttc
    59581 agtgttggaa cggtgacttt agtacccagc gccgcttccc acgggctaac cggcaccact
    59641 atttccagat cctggccgac aatatcaaac agcggatgtg gcgcaatgtg aatcaccagc
    59701 cacaggtcgc catttggacc gccgttttcg cccggcgtcc cctggccttt caggcggatg
    59761 cgttgaccat tgccgacgcc cgccgggatc ttcacattca gcgttttcgg gatttcctgt
    59821 tcgatcatgc caaaggcgtt ataaaccggc aggttatagc tgatggtacg cttatgctca
    59881 gtaagcgttt cttcgaggaa aaccgccact tcgatttcaa tatcgtggcc gcgtgtggcg
    59941 gggcgttgac ggctctggcg ggcatgctga ccgaaaattg acgagaagat atcgtcaaaa
    60001 tcttcggcgt taaaactctg accgtcgcca tgatggaact gacggttaaa ttgcggatcg
    60061 ttgcgatgtt gccacatctg atcatactca gcgcgacgtt gttcatcact taacacttcc
    60121 caggcttcgg cgacctcttt gaagcgggct tcggcatccg gttctttgct gacatcagga
    60181 tggtatttgc gggccagtcg acgataggcg gtcttgattg tcttgagatc gtccgtcggt
    60241 ttcacgccca tgatggcgta ataatccttt aattccatag cgttatctcg cgtaaatcaa
    60301 cacaaattga aggaacccct gtaaggtaac tcctataagt gtagggtaat cctcaaaatt
    60361 tcatatgcca acacagaata tgttattgaa atcatcgcgg aggggaggtt gcctcaaggt
    60421 gggaggaaca tattttattt acctcactcc tgaacttttg gctcatgctt acaagttaaa
    60481 caataaggaa aggaatgcac aactcgacta tttaaatcgt tatgcaattt aatgataaag
    60541 acatgtgcta aaaaccattg ctattagtct cacattcttt ttttattggt aatggtaacg
    60601 ccgctgattt tctttatagc tactgatgta atcattagcg gcgtaaacga ggccggggct
    60661 aatcaggcat agtttgcgtc aaaacttgcc tgtttttaaa ggggaatata gaaaaacagg
    60721 cgttcaacga gccattttgc gaacctgttc ccggaaaaat gacatatttc tgtcacactc
    60781 tttaattatt gataacaaaa gaggtgtgag gaatgaacaa aacgctaatc gccgcaactg
    60841 tggcagggat agttttactc gcttcaaatg ctcaggcaca aaccgtaccg gaaggctatc
    60901 agctacagca agtccttatg atgagccgtc ataacttacg tgcgccgctg gcgaacaatg
    60961 gcagtgtgct ggagcagtcg acgccgaata aatggcctga atgggacgtc cccggtgggc
    61021 agctcaccac taaagggggc gtgctcgaag tgtatatggg ccattatatg cgtgaatggc
    61081 tggcagaaca ggggatggtg aaatcagggg aatgcccgcc accggacacc gtttatgcct
    61141 atgccaatag tctgcaacgc accgttgcga ccgcacagtt cttcattacc ggcgcattcc
    61201 cggggtgtga tattcctgtt catcaccagg aaaaaatggg cactatggac cccaccttta
    61261 acccagtgat caccgatgat tccgccgcat tcagtgaaca ggcggtggcg gcaatggaga
    61321 aagagctcag caaactccag cttaccgaca gctacaagct actggaacaa atcgttaact
    61381 ataaagattc tcctgcgtgt aaagagaagc aacaatgttc gcttgtagat ggcaaaaata
    61441 cctttagcgc gaagtatcaa caagaaccgg gcgtttccgg gccgctgaaa gtcggcaact
    61501 cgctggtaga tgcgtttact ttgcaatact acgaaggttt tccgatggat caggtggcct
    61561 ggggagaaat caaatctgac cagcagtgga aggtgttgtc gaagctgaaa aacggttacc
    61621 aggacagcct gtttacctca ccggaagtgg cgcgcaatgt tgcgaaaccg ctggtcagtt
    61681 atatcgacaa agccctggtc actgaacgca ccagtgcacc gaaaatcacg gtattagttg
    61741 ggcacgactc caacattgcc tctctgttaa cggcgctgga tttcaaaccg tatcagttgc
    61801 atgaccagaa cgaacgcacg ccgattggcg gcaaaatcgt tttccagcgt tggcatgaca
    61861 gcaaagccaa tcgcgatttg atgaaaattg aatatgtgta ccagagtgcg gaacagttac
    61921 gtaatgccga tgcgttaacc ctgcaggcac ctgcgcagcg cgtgacgctg gaattaagcg
    61981 gttgcccgat agatgccaac ggtttttgcc caatggataa gtttgatcgc gtattgaatg
    62041 aagcggtgaa ataacagaaa actcccccgc gtgaagcggg ggagtcgctg gttaaacgtt
    62101 tttacgttcg atggtctgtt cgccccaaaa aagcgaatct ttatcggtct tagcaaaggc
    62161 tttgactaac acttcatcgc taccttcttc ccaaatcttt tccgccattt tttcgtcgta
    62221 cccggcgact tcgaaaatgg cctcggctat ttccggcgac gtattgcgca gagatgccca
    62281 ttcaccgacg tgatgagctt tcgcttcttg agttggcatg cgtatcctcc tgttgaagat
    62341 tagccgttaa gtttaactgc cagacctgcg acatattccc cttgataacg agcaatagac
    62401 agttcttcct ggctgggctg gcgtgaaccg tcaccgcctg cgatggtggt tgcgccgtac
    62461 ggcgtaccgc cgcgaacctg tgaaacgtca aataattcct gcgctgcgta gccaatgggg
    62521 acaatcacca tgccgtgatg cgcaagggtc gtccaggtgg atgtgatggt ttgttcctga
    62581 ccgccgccag taccggtgga actaaagacg ctcgccagtt ttccgtatag tgcgccggaa
    62641 gcccacaggc cgcccgtctg gtcgaggaag gtacgcattt gaccggacat gttaccaaag
    62701 cgggtaggtg taccaaaaat aatggcgtcg taatcggcca gttcttgcgg ggttgcaacc
    62761 ggtgcagttt gcgttttacc gcctgctttt tcaaataatt gcggcggcat ggtttccggt
    62821 acacgcttaa cgacaacttc tgcgccatcc actttgcttg caccctcagc aactgcgcga
    62881 gccatcgttt caatatgtcc gtacatggaa taataaagca ccagaacttt agccatttct
    62941 aaccactcct cgtgttatct ctattccgta gcgattcgct accacttatt taaagataag
    63001 acgtcctttt cagagtgcaa atttcacaac cacttatttg atttataaca actttcacaa
    63061 gcacgtaatt ttgtcgcaaa atgacacatt tttatctcat cgcgtttttt taatcataag
    63121 agcggcttat ggataattat tggagatgat atctattctc gctaagaagc tgttgcagga
    63181 tattaccaaa cgcggatctg cccgcgtcag ttcactaagc ttagttccac gtagcgaaaa
    63241 tatggcagcc gccatacgcc gcgttaattc tatgcaatat gatgtctata cccagacgga
    63301 ggtcagtaat ggcaaaccat cgaggcggtt ccggcaattt tgcagaagac cgcgaaagag
    63361 catcagaagc aggtaaaaaa ggtggacagc acagcggagg taatttcaaa aatgacccgc
    63421 agcgcgcatc tgaagcaggt aaaaaaggtg gcaagagcag tcacggtaaa agcgacaact
    63481 agccgggcta atcaatgacg catgcatttt tgtctgtagc tcgtcaaaac gccatcaccg
    63541 ccggtagccc cggcggtttt ctgtttatgg cttctgatga acaacttctg gcggtggaac
    63601 gtcaaccaac cttctgctta acaacgcatt gagtaaaatc gcgccaaagg tggctgtacc
    63661 gatccctccc aacgtaaaac cgcccagtgt gagagcaaaa tcacccgcac ccagcactaa
    63721 ggttactgcg accataatca aattaccgtt ctggcttaaa tccacactgt tttgtaccca
    63781 gatccgtgcg cctgcgacgg caatcagccc gaacacaaca attgatgcac caccaatcac
    63841 cgcagccgga atagtatgga tcagcgcgcc aaatttcggc gaaaagccca acagcatggc
    63901 gatgacggca gcggcaacaa acaccagcgt cgagtagact tttgtcacgg ccatcacacc
    63961 gatattctcg gcgtaggtgg ttatgccgct accgccgaca gagccagaaa gcatcgtcgc
    64021 cagcccatcg cctacgaatg cccgccccat atacgggtcc atattgcgtc cggtcatccc
    64081 ggcgaccgcc ttgagatgac ctaagttttc cgccaccaga atcaccgcca cgggcgcaat
    64141 cagcatcatt gcctgaccat taaaagcagg agtggaaaaa tgtggcagac cgaaccaggc
    64201 agcatggctg acgagagtaa aatcgacggc ttttcccagc cctaaaacgt tggtcatcac
    64261 gccatacagc agacaggcga caattaatcc tacgagaatc aataatcgct ggatcatgcc
    64321 acgggtaaat acggccacca gcccaataca cagcaccgtc attaccgcca tccagctatc
    64381 aaaggccgaa gccgatacat ttttcactgc gataggcgct aagttcaggc caatcgccat
    64441 cactaccgca cccgtcacaa ccggcggcat tagtcgttca atccagcgcg taccgatttt
    64501 catcaccacc aggccaatga cggtataaac cagcccacag gcgataatcc cgcccagcgc
    64561 gatgctgatg ttcggattaa ttccctgacc attaaagccc gtcgcggcaa tcaccacgcc
    64621 gacaaaagcc gcgctggaac cgagataact gggcacgcgc ccgccggtaa taaagaaaaa
    64681 cagtaacgtg ccgatccccg acattaaaat ggaaagattg ggatccagcc ccatcagaat
    64741 cggcattaac accgtcgcgc caaacatcgc caccgcgtgt tgaacgccca ttactgccgt
    64801 ttgagcaaac ggcaatcgtt cgtccggcgc gaccacgccg ctctctgtag aggtcgattt
    64861 taactgccag tgaggaaaac cgaacattgc catcagctgt ctccttaagg aggttaacaa
    64921 gcagggcgca tcagcgcgtg ataactgcga tcgaaccaca ccagcccgta gggtgtggcg
    64981 tgacgatgaa tcgcttcgat ggcgcaaaac aaaatgtcgt gggtgccgac gctcaccacc
    65041 tggctgatac ggcagtcaaa cgaaaccagt gccttttcca gttgcgggca tccggtcaca
    65101 ccagtctgcc agcgggcggc ggcaaagcgg tgttccatgg gcgttttgcc gccaaaaagg
    65161 tttgaaagcg gctcctgccc ggcgctaagt gtatttacac acagcgttcg attttcattg
    65221 aataccggcc agacggacgc cccacgattc aggcacacca gtaatgtggg cggcgtgtcg
    65281 gtcacgctgc agacggcgct ggcggtgaac ccggcgcgcc cggctggacc gtccgtagtg
    65341 atgatattga ccgccgcgcc catacaggac atcgcatcgc gaaaggtttg ttgatcgaca
    65401 atgttcattg tttgctcctt acaacagccc gcaggcttct tcaaaggaca gacgtggcag
    65461 gcgcgcaaaa agtttgctgg aatcgccata gccgatatta atcagcagat cgctcttcag
    65521 cgtgctgccc gcaaaaaagg cgtcgtcaac gtattgacgg tcaaagcccg acatcgggcc
    65581 ggtatccagc cccagcgcac ggcaggcgac gatcagatag gccgcctgca tggaactgtt
    65641 gcgaaacgct gtttcttcgg caagttgtgg actggaggta aaccaactgc gggcatcgcc
    65701 gtgaggaaac agctgtggta accgttcata aaattcactg tcccaggcga cgatagcggt
    65761 gacgggcgcg gtcagggttt tttgcagatt gccgctggaa agcgccgggc gcagacgttc
    65821 ttttccttct gcagtgcgga taaacacgat ccgggccgga gaacagttag ctgatgtcgg
    65881 cccccatttc atcagggcat aaatctcccg taacgtctca tcgctgacgg ggggctcccg
    65941 ccagccgttg tgagtgcggg catcggtgaa cagggtgcta agcgcacctg ggctaacggc
    66001 ttcgttcata gcaattcctt acagagcggc ttcacggtga tgtaacaggc tggcaagccc
    66061 gttgagtaac agagcattaa acgtttcggg atcggtcacg ttgcaggcgt gtccgccata
    66121 gcgcatcacc attttctggc tatcgggcag ggcggcatga agttcactgg aacatgctgt
    66181 tggcaccagc agatcgtcac tggcgcagat gatttgcacc gggcagcgga tacgatccgc
    66241 atggtgacta aagtcagcgc gtttgagggc gttaagtcga cgcagtaaat tatttttgcc
    66301 ctgaaaatgc gccagtgcca gcgcatcttc tgcctcaagg cgaggtgcgc gggccgccat
    66361 ccagtcggcg ggatagagga acaacggctg cgcttccacc catgcctgcg cgccgccgct
    66421 atgtagtaac tgttcgcgaa cctgaaaaca gcggcgcgta tgggtgttta ttcgtagcca
    66481 gccgttaacg ctgaccagca cagttaccga cgcgggataa tccagcgcca gctgcattcc
    66541 caccagcgca ccgagcgcat ggccgaccac cgcgtaacgc tcaatccctg cggctaccag
    66601 cgcctgatgc agttccgctg ccatctgggc gatactgtaa tcttctgcca gcgtgtcggg
    66661 attattgccg gtgccgcgct ggtcgtaaca gactacctga tactcctgat ccagcaccgc
    66721 cagttgcggt aaccagtaac tgccgctacc gccaagaccc gaaatcaaca ccactacggg
    66781 cgcatcagca taagggggag gtgagagtga aagtttcatc gcggcctcac ttggcgatat
    66841 gcgcaattgt ggcgatttcc accagcgcgt caggttttac cagtccgcac tgaatgcaga
    66901 atcgcgccgg tttgtcaccc gggaaaaact cggcgtagat ttcgttaatc gcggcgtaat
    66961 ttttccagtc ggtaataaag atgctgttga aagtcacatc cgccatcgtg ccacccgccg
    67021 tctcgatgac cttgcggatg gtttccagaa cgtggcgggt ttgcgccttt gggtcatcgg
    67081 caaacagcac gttgttatgt tgatcaaaag ccagcgtacc ggagacatac accacgccat
    67141 cagccagcgt gccgggaacg aagggggcca gcggtgcgct gctgccagca ggaataatta
    67201 cggattttgg catcgttaaa ctccttaagc gatacgagca aaggacgtgg aagaaagcgc
    67261 gtcgcagaac gtttcgacgt cgctgaccca gccaaaaaag gtttcgatat tgaacaacgc
    67321 agctttctgt gcaaattccg gccccgcctg gtgtgttgcg tcttccagca ccacgccgaa
    67381 atactccaga aaaaagccgt cgcgtagcgt cgattcgacg cagacgttgg tagcgatacc
    67441 ggtgaaaacc agatggcgta ttccacggct gcgcaaaatg ctgtccagcg gcgtattgaa
    67501 gaaaccgctg tagcgcggct tcggcagcac aatatcgcca ggctgcggca ccagttcatc
    67561 caccagttga taatcccagg agcctttcgc cagcaatttc ccctgcagct gcggctgctt
    67621 acgcatggtt ttcagggcgt tcgatttatg aaaattcggt gagccggggc cgccagcctc
    67681 gacatactgt tcatcccagc cattttgaaa ccagatgatc agcatccctg ccgctcgcgc
    67741 tgcggtcacg gcggtttgaa tgttggcaat gaccgggcga gtggttgaga catcaaaccc
    67801 ggcgagatct aagtagccgc ctggcgtggc ataagcgttt tgcatatcca ccacgatcag
    67861 cgcactttgc tgcggatcga aggtaatggc ttccggtcga gcggttaagg tcgtcatcat
    67921 gccacctcct gagtcagcgc agggagatgg gcgcggcact gcatcagtgg ttgaatgcgc
    67981 tcgccgaagg tttcgattcc cgacagaaaa tcatcgaagg tcaacagcac gccttcggca
    68041 ccaggcacgt ttgcgacttc atctaacatg cgcgcgacac tggcgtaaga accgactaac
    68101 gtccccatat tgatgtttac cgccgaagtg ggatcggcca tctgacggac gttggtgtca
    68161 gtacctgagc gggtatcttt ctgactttgt tcggttagcc agcttaacgc ctcttcatcc
    68221 gcgcccgctt tgtagtgttc ccattttgca cgagcggcat cgtcggtttc gtcggcaatc
    68281 accataaaca acacataaga gccaacgtcg cgtccggttt gctctgcggc ctgtttcatc
    68341 cgcgcagcgg tcggggcgaa agccgtgggt gtatttacgc ctttgccgaa acagaagtta
    68401 aaatcggcat accgggcgga gaacgccatg ccagcgtcgc tttgcccggc gcagatcact
    68461 ttcatgggga cactcggttg cggactgacg cgacaatcat ccatagtgaa aaaatcgcct
    68521 ttaaaatcgc ttttccccga gccccacagg tcgcgcagca cctgaacata ttcggtgaga
    68581 tagtcgtaac gacgggagaa atagtcatcg ccaggccaga tccccatctg ctcatactcg
    68641 ggcttctgcc agccagtcac gaggttgacg ccaaaacgcc cgccagagat ggagtcgatg
    68701 gttgcggcca tacgggcgac gattgccgga ggtaacgtta aggtggcagc tgtcgcgtat
    68761 atctgaatgc gcgaggtcac ggctgccagc cccgccatca aggtgaacga ctcaaggttg
    68821 tgatcccaga actcagtttt gccgccaaag ccacgcagtt tgatcatcga cagggcgaaa
    68881 tcgaaatggt agtgctccgc tttttgcaca atggctttat tcagttcaaa tgtcggcatg
    68941 tactgcggcg cgtgggtcga aatgagccag ccgttgttgc caataggtac gaatacgcca
    69001 attttcatca tcaacctctc ttcgtctcgt aaagtgaaag tcagacgggg cgctgcatcc
    69061 tgcatatcct tttcagccgc gtaatggctt gtttgcaaag cggatgccag tttttaaaaa
    69121 gtgaatgtta ttaatgtgtt aatgttaagt tgtctaaaat gcctgaaaaa aagtggacta
    69181 aacggtcaaa acagttgcac ataaaacatg cattcgtgcg cgatgagagt gcagaaggtc
    69241 gaggccgggc gggggttttg ctatcctgtt gccaatctac aagaggggag agcgcatgac
    69301 gcaaggcgca gtgaaaacaa cgggtaaacg ttcgcgcgca gtgagcgcga agaaaaaagc
    69361 gattcttagc gcagcactgg acactttttc acaattcggt tttcacggca caaggctgga
    69421 gcagatcgcg gagttggcgg gtgtttcaaa aaccaatctg ctgtattact ttccgtcaaa
    69481 agaggcgctg tatatagccg tactgcggca gattctcgat atctggctgg caccgttaaa
    69541 agcgtttcga gaagatttcg ccccgctggc ggcgatcaaa gagtacatcc gtctgaagct
    69601 ggaagtttcc cgcgattatc cgcaggcttc gcgcctgttc tgtatggaga tgctggcagg
    69661 tgcaccgctg ttaatggatg aactgacggg cgatttgaag gcattaattg atgagaaatc
    69721 ggcgctgatt gccggttggg ttaaaagcgg caaactcgcg ccgattgatc cgcagcattt
    69781 gatttttatg atttgggctt ccactcaaca ttacgccgat tttgcccctc aggtggaggc
    69841 ggtgacaggc gcgacgttgc gcgatgaggt atttttcaat caaacggttg aaaacgtgca
    69901 gcggattatt attgagggga ttcgaccacg ctaatggtgc cggaggaagg tgtaacatcc
    69961 tccggctacc tgtttaaccg atcgtcatta aactggcgtt accgccagcg gcagcggtat
    70021 tcacactcag cgaacgctcg atatacagcc gttccagaag gatattgctt tcgccacggg
    70081 caaaaccctg caccgaaaca attgcaccat cccgcgcggc aacggcttca cacaatgcgc
    70141 gaagctgatc cgaatcaccg tggaagatca ccgcatcaaa tggttgagcg gttatatttt
    70201 ccgctttcgc cagttgaata cgttcgctga ctgccgatgg caatgccttc actaactgac
    70261 gatgcagcgc attatccggc cacagcacct ggctacccac cgccagcacg gcggcgagct
    70321 gagtcagcgc atcctgttca tcatccgcaa tacacaacac gcgctcacgc ggcagcagcg
    70381 tccaggtgtt gcgttcaccc gtcggccctg gcaacaatcg ttgtgttcct gcctgcgcca
    70441 actcgccata ttgcgtacat aacgcctgca attctggacg gtttgccgcc cattcccgca
    70501 gtgcatttag cggctgagtc aatgcggctt tcaactgcgc atcgaccgga tactccgcat
    70561 cctgacgcgc gagcgtcacc gccagcgcac tttccgggcg attcgccagc agacggtaga
    70621 gatagagcgg accgcctgct ttcggcccgg tgccggacaa tccttcgccg ccgaacggtt
    70681 gcaccccaac cactgcgccc accatattac ggttaacgta cagattaccg acatgcgccg
    70741 agccagtgac ctgggcgatg gtttcatcaa tgcgcgtatg gacgccaagc gtcagaccat
    70801 aaccggaagc gttaatctgc tcgatcagct ctgggagctg gttacggttg taacgcacca
    70861 catgcaacac cggaccaaag acctcttttt gtaattcggc aaagtcatcc agttcgatca
    70921 gcgtcggagc gacaaaggtg ccgctttgcc attcacgggc atcttcgctg ttttcccgca
    70981 ccgcctggaa cacctgacgg cctttactac gcatggtctg aatatgacgc tcgatattgg
    71041 ctttcgcttc gctatcaatc accggaccga tatcggtggt caggcgaccc ggattaccca
    71101 tccggcattc ggccattgcg ccgcgcagca ttttcagcgt gtggtcggca atctcatctt
    71161 gcaggcacag cacgcgcagt gccgaacaac gctgacctgc actgtcgaac gccgaggcca
    71221 gcacatctat tacgacttgt tcggtcagtg ctgaagaatc gacaatcatc gcgttcatgc
    71281 cgccggtttc agcgatgagc ggaatagggc gaccctgagc gtccaggcgg ctggcgatat
    71341 tgcgctgcag taacgtagcg acttcggttg aaccggtaaa catcaccccg cgcacgcgat
    71401 catcacccgt cagttgcgcg cccacggttt caccccgacc tggcagcaat tgcaccacgc
    71461 ctggcggtac acccgcttcc agcaaaatgg cgatcccttg cgcggcaatc agcggcgttt
    71521 gttctgccgg ttttgccagc acgctgttac ctgccgccag tgcggcagcg atctgtccgg
    71581 tgaaaatagc cagcgggaag ttccacggac tgatacatac cacaggccct aatggacggt
    71641 gggtttcgtt agcgaaatca tcccgcacct gtccggcata gtagtggaga aaatcgaccg
    71701 cttcgcgcac ttcggcaatg gcgttactga aggtttttcc ggcttcacgc accagaatac
    71761 caatcagttg ctgcatctgg ctttccatta gcacggcagc gcgatgcaaa attgccgcgc
    71821 gttcagccgg aggagtggca aaccagattg gcgcgttatt aaccgcactt tccagcgcct
    71881 gttctacttc acgcggcgtg gcttcacgca catagcccac aatatctttc ggttccgcag
    71941 ggttaataac gggcgacatc tcacctgccg ctaccggttg ttccagcatt ggcaaggact
    72001 gccatttttg cagtgcacta ttgagcaggg ctgaagagag cgaggccaga cggtgttcgt
    72061 tagcgagatc cagccctgcc gagttgtcgc gcccgtgacc gtaaagatcg cgcggcaggg
    72121 gaattttcgg atgcggtaat ccagtttgcc cttcctgttg cgccagtttt tctacagcag
    72181 tgactggatc ggcgaccagt tcatccagtg gcaaagaggt gtcggcaata cggttaacaa
    72241 acgaggtgtt agcaccgttt tccagcaggc ggcgcaccag atatgccagc agcgtttcat
    72301 gtgtaccgac cggagcataa atacgacacg gacggttaag tttgccgtcg gcaactttcc
    72361 cggtgacctg ctcatacagt ggctcgccca taccatgcag gcactggaac tcgtactgac
    72421 ccgggtagta gttttgaccc gccagttgat aaattgccgc cagcgtatgg gcgttgtgcg
    72481 tcgcgaactg cgggtagatt agatttggca ccgccagcag ttttttcgca caggcaagat
    72541 aagaaacgtc ggtatacacc ttgcgggtat aaaccggata accttcaagg ccgtccatct
    72601 gtgcacgctt gatttcacta tcccagtacg cgcctttcac caggcgaatc atcagacggc
    72661 gacggctgcg ggtggcgaga tcaatcaggt aatcgatcac caacgggcag cgtttttgat
    72721 aagcctgaat aacaaaaccg atgccgttcc agcctgccag ttcaggctcg aaacagagtt
    72781 tttccagcag atcgagggag atctccaggc gatcggcctc ttcggcatcg atgttaatac
    72841 caatatcgta ctgacgcgcc agcagggtga gtgatttcag acgcgggtaa agctcttcca
    72901 ttacccggtc atactgggcg cggctataac gcggatgtag cgccgacagc ttgattgaaa
    72961 tgcccggacc ttcatagatg ccacgaccgt tagacgcttt accaatggcg tgaatcgcct
    73021 gctgatagga aaccatatac gcctgtgcgt ctgcggcagt cagtgcggct tcgcccagca
    73081 tatcgtaaga gtaacggaaa cctttctctt ccagtttgcg ggcattggct aacgcttccg
    73141 cgatggtttc gcccgtgacg aactgctcgc ccatcaggcg catcgccata tccacacctt
    73201 tgcggatcag cggttcaccg ctcttaccga taatgcggtt cagcgagcgg gagaggctgg
    73261 cttcgttatg ggtggaaacc agtttgccgg taaacagcag cccccaggta gcggcattaa
    73321 caaacaggga cgggctacga ccaatgtgtg actgccagtt accgttactg attttgtcgc
    73381 gaattaacgc gtcacgggtg gctttgtcgg gaatacgcaa caacgcttcc gccagacaca
    73441 tcagcgccac gccttcctgc gatgacaacg aaaactcctg caataacccc tgaaccatac
    73501 ctgcgcgacc actggcgttt ttttgattac gcagtttatc ggccagttga tacgccagtt
    73561 tgtgcgcctg ttcagcaact ggctgcggca ggcgagcttg ttccagcagc atagaaaccg
    73621 cttcggtttc cgggcggcga taggccgcgg tgatcgcggc gcgggaaacc gactggggca
    73681 atatttgttc ggcaaagtcg aggaaaggct ggtgtggttc ctctgccgga gtcggtgctt
    73741 catcgctctc attggccgcg ccagaaagca gcgcaggtag ctccgggaga gtatcgctgt
    73801 tttccagttg ttcaagataa gaaaaaatcg cctgtttaat taaccagtgt ggtgtgcgat
    73861 cgatacgtgt cgcagcagac ttaatacgct cacgcgtcgc gtcgtccagc ttaaccccca
    73921 tggtggtggt tcccatgcca ttactcctgt tgttctgaaa ggtgcaactt aacgttatcg
    73981 tgaaatatcc atgatgttgc aactttgtgc aaccatgtta aatgtgacct gcgtagcaag
    74041 cttaaaaatg aatgaaatgt taataaacga aatcgatatg acagggatta aaaaaataac
    74101 tcagactttt tctctgcggc agttaacatt tttgaaaggt gcaaccgcaa aaaatgtgag
    74161 agagtgcaac ctgatgaaaa atagtgtcgc tgagcactaa aatttaatgt aaatggtgtg
    74221 ttaaatcgat tgtgaataac cagcgcttcc ggcaggatac ggtcgccctg gtaaaacata
    74281 aactctgtta ccccgttccg gtggcagata taacggcaag tttcgacatt gccgataata
    74341 attttttgga gactttagat ggctattagc acaccgatgt tggtgacatt ttgtgtctat
    74401 atctttggca tgatattgat tgggtttatc gcctggcgat caacgaaaaa cttcgatgac
    74461 tatattctgg gcggtcgtag tcttgggcca ttcgtgacgg cattatcggc gggtgcgtcg
    74521 gatatgagcg gctggctgtt aatggggttg cctggcgctg tttttctttc cgggatttcc
    74581 gaaagctgga tcgccattgg cctgacatta ggcgcgtgga tcaactggaa gctggtggcc
    74641 gggcggttgc gtgtgcatac cgaatacaac aataacgcct taacattgcc ggattatttc
    74701 accgggcgct ttgaagataa aagccgcatt ttgcgcatta tctccgcgct ggttattttg
    74761 ctgttcttca ccatttattg cgcttcgggc attgtggcag gcgcgcgtct gtttgaaagt
    74821 acctttggca tgagctacga aacggctctg tgggcggggg ctgcggcgac gatcctttac
    74881 acctttattg gcggtttcct cgcggtgagc tggactgaca ctgtacaggc cagtctgatg
    74941 atttttgccc tgatcctgac gccggttatc gtgatcatta gcgtcggtgg ctttggcgac
    75001 tcgctggaag tgattaaaca aaagagcata gaaaacgtgg atatgctcaa agggttgaat
    75061 tttgtcgcca ttatctcgct gatgggttgg gggctgggtt acttcgggca gccgcacatc
    75121 ctggcgcgtt ttatggcggc agattctcac cacagcattg tccatgcgcg tcgtattagt
    75181 atgacctgga tgatcctctg cctggcaggg gcggtggctg ttggtttctt tggtatcgct
    75241 tactttaacg ggcacccggc ggtagcaggt gcggtaaacc agaacgctga acgcgtgttt
    75301 attgaactgg cgcaaattct gtttaacccg tggattgccg ggattttgct gtcggcgatt
    75361 ctggcggcgg taatgtcaac gttaagttgc cagctgctgg tatgttccag tgcaattacc
    75421 gaagatctat acaaagcatt tctgcgtaaa caggccagcc agaaagagct ggtgtgggta
    75481 gggcgtgtga tggtgctggt ggtggcgctg gtggcgattg cgctggcggc aaacccggaa
    75541 aaccgcgtgc tgggcttagt gagctacgcg tgggcaggct ttggcgcggc gtttggtccg
    75601 gtggtgctgt tctcggtgat gtggtcacgc atgacgcgca acggtgcgct ggcggggatg
    75661 atcatcggtg cgctgacggt tatcgtctgg aaacagttcg gctggctggg tctgtacgaa
    75721 attattccgg gctttatctt cggcagtatc gggattgtag tgtttagttt gctgggtaaa
    75781 gcgccgtcag cggcgatgca aaaacgcttt gccgaggccg atgcgcacta tcattcggct
    75841 ccgccgtcac ggttgcagga agggtaaggg aaggcgtttt accgaaaatt tcacgtcata
    75901 acaggaagca ggcagagggc aatcagtgat tgccctctgc gttttttctc aaaatatttt
    75961 tacatcttct catcattatc actacatttc acttgtattt ttcttcatcg taatgataat
    76021 cgctatcact gcgatttact tttctttgca tagattgact aagaaaaacg tttaagggtg
    76081 ggtggcatgt ttgttccgtt tctcattatg ttgcgcgaag gacttgaagc cgcgctgatt
    76141 gtcagtttga ttgccagcta tcttaagcgt acccagcgag gccgatggat tggtgtaatg
    76201 tggattggcg tgttgcttgc cgctgcgttg tgcctgggct tgggtatctt cattaacgaa
    76261 accaccggcg aatttccgca aaaagaacag gaactgtttg aaggcattgt ggcggtgatc
    76321 gccgtggtga tccttacctg gatggttttc tggatgcgca aagtgtcgcg caacgtcaaa
    76381 gtacaactgg agcaggcggt cgatagcgca ttgcagcgtg gaaatcatca tggctgggcg
    76441 ctggtgatga tggtcttttt tgccgttgca agggaagggc tggagtcggt ctttttcctg
    76501 ctggcggcat ttcaacaaga tgtcggggtc tggccgccgc tgggtgcaat gctcggtctt
    76561 gctacagccg tggtgcttgg cttcctgctc tactggggcg gtattcgcct caatcttggt
    76621 gcatttttta aatggaccag tctgtttatt ctcttcgtcg ccgcagggct ggcagctggt
    76681 gccattcgcg catttcatga agccggattg tggaaccact ttcaggaaat cgccttcgat
    76741 atgagcgcgg tgctctcaac tcactcgctg tttggcacac tgatggaagg gatttttggc
    76801 tatcaggaag caccgagcgt cagcgaagtc gccgtctggt ttatttatct catcccggcg
    76861 ctggtggcat ttgttctgcc accacgcgca ggggcgactg cgtcccgctc cgtgtagcaa
    76921 atacgacgca aactcttgct tagttacaac atactttaaa gggatagtct cgtcatgacc
    76981 attaacttcc gtcgtaacgc attgcagttg agcgtggctg cgctgttctc ttctgctttt
    77041 atggctaacg ccgccgatat tccgcaagtc aaagtcactg tgacagataa gcagtgcgag
    77101 ccgatgacca ttacggttaa cgccgggaaa acacagttca ttattcagaa ccacagccag
    77161 aaggcgctgg agtgggagat cctcaaaggc gtgatggtgg tggaagagcg ggaaaatatc
    77221 gcccctggtt ttagccagaa aatgacggcg aatttacagc ctggcgaata cgatatgacc
    77281 tgcggtctgc tgactaaccc gaaagggaag ttgatcgtca aaggtgaagc aacggcggat
    77341 gcggcgcaaa gtgatgcgct gttaagtctt ggtggtgcaa ttactgcata taaagcgtat
    77401 gtcatggcgg aaaccacgca actggtgacc gacaccaaag cctttaccga cgctattaaa
    77461 gcaggcgata tcgaaaaagc gaaagcactg tatgcgccga cgcggcagca ctatgagcgc
    77521 atcgaaccga ttgctgaact gttctccgat ctggatggca gcattgacgc ccgtgaagat
    77581 gattacgagc aaaaagccgc cgatccaaaa ttcaccggtt tccaccgtct ggaaaaagca
    77641 ttgtttggcg ataacaccac caaagggatg gataagtatg ctgaccagct ttataccgat
    77701 gtggtcgatt tgcaaaaacg catcagtgaa ctggctttcc ctccttcaaa agtggtcggc
    77761 ggtgcagccg gactgattga ggaagtggcg gccagcaaaa tcagcggtga agaagatcgc
    77821 tacagccaca ccgatctgtg ggatttccag gctaacgttg aaggctcgca gaaaattgtc
    77881 gatctgctgc gtccacaact acaaaaagcg aatccggaac tgttggcaaa agtcgatgcc
    77941 aacttcaaaa aggtcgatac cattctggcg aaataccgca ctaaagaggg ttttgaaaat
    78001 tacgacaaac tgaccgatgc cgaccggaac gcactgaaag gaccgattac tgcgctggcg
    78061 gaagatctgg cgcaacttcg tggtgtgctg gggctggact aagcgttatg cagtatgaag
    78121 atgaaaacgg cgtgaatgaa ccgtcacgcc gacgtttact gaaagggatt ggtgcgctgg
    78181 cgctggcggg aagttgtccg gttgctcatg cacaaaaaac gcaaagtgcg ccgggtacgc
    78241 tttcaccgga tgcacgcaat gagaaacagc cgttttatgg tgagcatcag gcagggatcc
    78301 tgacaccaca acaggctgca atgatgctgg tggcgtttga tgtgcttgcc agcgataaag
    78361 ccgatcttga acggttgttt cgcttgttga ctcagcgttt tgcttttctg actcagggcg
    78421 gagcggcacc agaaacgcca aatccgcgcc tgccaccact cgattccggc attcttggcg
    78481 gctacattgc gcccgataat ctcaccatca cgttatcggt gggtcactca ttgtttgatg
    78541 agcgctttgg ccttgcgcca cagatgccaa aaaagttgca gaagatgacg cgtttcccca
    78601 acgactcgct ggatgcggcg ttatgtcatg gtgatgtgtt gctacaaatt tgcgccaaca
    78661 cccaggacac ggtgatccat gcgctgcgcg atatcatcaa acacacgccg gatttgctca
    78721 gcgtgcgctg gaagcgggaa gggtttattt ccgaccacgc agcgcgtagt aaaggcaaag
    78781 agacgccgat aaatttgctg ggctttaaag acggcaccgc caatcccgat agccagaatg
    78841 cgaagttgat gcaaaaagtg gtgtgggtga cggcagatca gcaggagcct gcgtggacaa
    78901 tcggtggcag ctatcaggca gtacgcttga ttcagtttcg agtggaattt tgggacagaa
    78961 cgccgctgaa agagcagcag acgatatttg gtcgtgataa gcaaaccggt gcgccgctgg
    79021 gcatgcagca tgagcatgat gttcccgatt acgctagcga tccggaaggg aaggtgattg
    79081 cgctggacag ccatatccgg ctggcgaatc cccgcacggc ggagagcgag tccagcctga
    79141 tgctgcgtcg tggctacagt tattcgttgg gcgtcaccaa ttccgggcaa ctcgatatgg
    79201 ggctactgtt tgtctgctac caacacgatc tggaaaaggg cttcctgaca gtacaaaaaa
    79261 ggttgaatgg cgaggcgctg gaggaatatg tcaaacctat cggtggcggc tatttctttg
    79321 cgctgccggg tgtgaaggac gcgaacgatt atctcggaag cgcattattg cgggtttaat
    79381 gtttttaggc gtataaggcg tttacgccgc accaggcgtt cgtgcgctga tgcgacgctt
    79441 gcgcgtctta tcatgcctac aatcggtgcg ggtcggtagg ccggataagg cgttcacgcc
    79501 gcatccggca agcttttacc agtaccgcga atcctgctga aagcacacag cttttttcat
    79561 cactgtcatc actctgtcat ctttccagta gaaactaatg tcactgaaat ggtgttttat
    79621 ggttaaatat aagtgaatat attgttgcaa tgaatgcgaa atctgttgta cttattaagt
    79681 agcagcggaa gttcccggca gtgatagtca gtcactatgg agatcgcgga tggtaacgtc
    79741 ctgtactgga catgttttag agaatcagcg cgccaccact cgcggagatt tctcctccgg
    79801 tagtcatctc gtcactttgt attttcaacc tcatcctttc ttttcatgtg ttaccgacgc
    79861 cgtaaacggt gcgcgtagcc gtttttcggc attttatcca aaagcaaact atggcttaca
    79921 aggaagccaa ccctctgatg ttcgtgcgca taatcgcgct gccaacggcg cgtgtgatga
    79981 atacaaacaa ctcaaggtgc tctccatggg aagacaaaaa gcagtgatca aagctcgtcg
    80041 cgaggcaaaa cgtgtgctga gacgggattc gcgcagccat aagcagcgtg aagaagaatc
    80101 ggtcacctcg cttgtgcaaa tgagcggcgt agaagccatt ggtatggccc gcgacagtcg
    80161 cgatacctcg cccatcctcg cgcgaaatga agcgcaattg cactatctga aggctattga
    80221 gagtaagcag ctgatatttg ccacgggtga agccgggtgc ggaaaaacct ggatcagcgc
    80281 agcaaaagcg gcagaggccc tgatacataa ggatgttgac aggattatcg tcacccgtcc
    80341 agttctgcaa gccgatgaag atcttggttt cttacctggt gatatcgcag aaaagtttgc
    80401 tccctatttt cgcccggtct acgacgtgct ggtccgtcgc ttaggggctt cctttatgca
    80461 gtactgcctg cgaccggaaa tagggaaggt ggaaattgcg ccgttcgcct atatgcgtgg
    80521 acgtaccttt gaaaatgcag tcgtcattct tgacgaggcg cagaatgtga ctgccgcgca
    80581 aatgaaaatg tttttaaccc gcctcgggga gaacgtgacg gttatcgtca acggtgatat
    80641 cacgcaatgc gatttgcctc gcggcgtgcg ctccggatta agtgacgcgc tggaacgttt
    80701 cgaagaggat gaaatggtcg ggatcgtccg attcggtaaa gaggattgcg tacgttcggc
    80761 actttgccaa cgtacgctac atgcatacag ttaagtgtat tatcggtgca gagcccgggc
    80821 gaaccgggct ttgttttggg tgtttatacc cggactagcg ttttttctga aaccaccatt
    80881 tttatttgtc cctggctggt gaaatgtacg ctcatcctgt ggcttttttg tagttgctga
    80941 taaagctcat caggtattgc taagctctct gcatattctt gcggcgtata ttggtatgct
    81001 gcatgatgct gctgtttttg aaaacgcagc ttattgtaca acgcccagac gattaacacg
    81061 acggcattca ccactgccag caaaaaatag aactgaagtc ggcttctggc ctcgctttgc
    81121 cagaaataac ccgtcagcag atcaatggcg aataagaata tgaataaagc aaataatgtc
    81181 cacaagatag ttgtggcaac ataatcaacc agtaaacgta ctggcgattg tcgggtcgta
    81241 ataattagat tgttcatatt aacctctcag aatcccacga tcgggactta cccaacgagc
    81301 gcgttgtttt ttaggcatca acatgactcg tgtaaatgat accaacgtcg ttgccaggct
    81361 tagcatccag aaaataaccg ggaaccaaat aatccagaaa agcgatgaag ccagattgtg
    81421 ctcatagcga ttctcaatca ccagactgac aataaattgc agtaaacata acgtacacaa
    81481 taatattccg gcagtatgtg tcgctgcgat atgtgtcaat tcaatattta acggtacacc
    81541 tgcaagttgg gctgcgtaaa taatgaaacc gaccaggcag gtaaaggccc atattgtcgt
    81601 cagacagtat tcaaaaaaca gtggccacat tcgaaagttt tctttgcgcc acaaccttgt
    81661 catatttttg aggaatactt ctgcaccgcc ctgagcccag cgcaggcgct gattccacag
    81721 cccttttaac gtttcaggca ttaatatcca gcacagcgcc cgtggctcgt aaaaaatcgt
    81781 ccactgattc aactgcagct tccagctaat atcaatatct tcggtgatca tatcgtcact
    81841 ccagtaaccc acttctgcca gggcgctgcg acgaaatgcg gcaataacac cggaaacggt
    81901 aaatacgttt ccatagatac gctgggtccg cttgatcaaa ccaataattg aggaatactc
    81961 gccaacctga attttaccca ccagggtaga acgtgtgcga atacgaggat taccggttac
    82021 ggcacccaca cgcgggttgt acaacatcgg ttccacaata tatgccgctg catcgcggtc
    82081 taataacgcg tcgccatcaa tgcacaccag atattcactt ttcgccgcgg cagctccggt
    82141 tttaagcgca atggctttcc cctggttttg cgccagatga atgacccgca gacggggaat
    82201 ttgtgcagcc atgcgatcca ggatggcacg ggttttgtct gttgaaccat catttacggc
    82261 aataacttca atgttctcat aacgctgtgc taaagcggca tgtatggttt cttcaacgtt
    82321 tttctcctca ttaaaacagg gaatgataat ggagatagat ggattatctt tcaactgggg
    82381 ggctggtgcg ttttctcccc acggccagtg gcgttcacga tagacccaga aatagacgcc
    82441 gccaataatc cacataatgg acataaaaaa cggccagaag aaaacgaacc tcatcatcag
    82501 ttcaccagag tgaaagtacg ctacgcatag gggtatgcat aacaccagac ataatataaa
    82561 aaacgatacg atgcgattaa tcatttttcg gataccaggc tgttgaaaac tcaggacgaa
    82621 taacgtctat ttcaggttgg ttatgcagaa aattgtcggg ataataacca taatttttca
    82681 ctccattcag ttgtaatagg ctcatccagt gagcgagttg ttgtgaagaa atagcctgat
    82741 gctgaccatt tttctgccag ttttgtgcct gtaattctaa aattgatttg tctttagcct
    82801 gcgggatgct tttcatctga ttggtcaatt gtattaacca ttgatcagcc gatttttctg
    82861 tgacgccttc catataaggc atagccataa tagcggtcca gtcatagctt tttaggaaat
    82921 cagcataatt ctgtgcaaac caggcttcac tttcaggttg tattaccgga agtgcaaaaa
    82981 tatttcgtgc agttttaaca tgtggaccac gaatggcttt tacgcgcgca ctaagttcta
    83041 aagtgaagtc agctaacgca cgacttttaa agcgggtcca ctgtttaaat tgctccgggt
    83101 tttgtcgaat ttcgctcaga ctcccgctaa agccagcttg ctgataagcc gcgatagccg
    83161 ctgcactggc atcttcataa tctgaaagca aagcatcatc gtggaacaat atgccatcaa
    83221 aagcagcatg tccggcaaga tcatcatata acataccaac ttgtgctctg accctgtcat
    83281 cgaaaggaga gagacggcgg tattgttcag gatgaatttg tgcttttttc tccccagttg
    83341 gtaagtattt tactcgcgtt aatgtgggat ctaaatccca gcttaatacc ggcatccacg
    83401 catagatgtt tacacctgag cgggtacgca attgccaggc aacccgacta aaaatatctg
    83461 ctttcattgg tagcaaacga tttggaaacc agacctcttt aaccagccca tcaccatcgg
    83521 gatcagcaaa tgcttgcaaa tacacggtag atatttgcat atcttttacc cgctgaatta
    83581 gcacatcaat attgcgatcc atttgctggc ggttttcgtc ataaacgtaa tcaagatcga
    83641 tatgcattac ccgttgtagt ggtttttcct gtacggtaat aatttgctgg gcaaactctt
    83701 ttaatgaggg attattggcg attaatactc gcggaatgga atctaattgc gacgcatttg
    83761 ccaaacctga ttcaagggtg aagaacatgt cataaccgag tttttttaat tccgctattg
    83821 ctatgccatt cgcttcgcca taaggccaaa caaaaacgtg tggatttacc tcagcctttg
    83881 tacgcaggta ttccgtcatt tttacagcat ccaggcgaat tctttcccgg tattctgctg
    83941 cggtttcata ccgtgcgtgg tcagtaaaat atgcacgatt tacatatgca ggcaataagc
    84001 tgccggtggc attagcctga ataccgtagt gagaatccca tgtatgagaa gcgacctcaa
    84061 cgagccggga acgcgcaact tctcgcactt gttgccacgt ggcaaaatat tctcgactga
    84121 ccatctcatc gccaaatttc acttgtttat ccgctggagt atcgacccaa ctgccgacgg
    84181 gagcccatac agccggccac tggaaggcct gaagaattgg gaagacacgg gtataaaaac
    84241 tctggtagcc gtcatcaaaa gtcagcacta ctgctttttc cggtagcggt tttcctcctc
    84301 gatgtgcttc acgaatttga gcaatactga ccggttgata accgttctcg cgcagccagg
    84361 caaattgttc acgcagtgct gatgttcgca ctgacataaa acgctggtcg gcagcttcgt
    84421 cttcaacgtt atgccatgaa atcaccacaa aaccattatg tggccacggt tgctcggcga
    84481 gtaaagattc gcgatcctgt ggcggtataa atgatgttct tgactggcta atgcacgcgg
    84541 tgagcataat tatactcacc agcatcagga gatatttatt tccattacgt aacatattta
    84601 tccttaaaat ctgaatgtca tatcgaattc aacgtataag ttgtgttctc tgtcaccgtc
    84661 ataaggtcgt ttttcccagc gtagcgttgc gccagcatca atcacgtcat tccagctaat
    84721 acgttgcccg tagccgagtt gggtgacgac atccgtgcca taatgttttt gccaggaggc
    84781 accaacacct gcgctgaata tttgctccca gctattttca tagcttcgcc ataacaaatg
    84841 acttgcctca aatgccggaa caatatcgaa catttttata gggttgtagt atggggtgtc
    84901 gtgttctgta ttttgttcgt aatacagatt gggtaggaaa tcgacaatca aatatggtga
    84961 agaccagatg cgttcttgac cctcaattga gacttcatga cgctggttac tgtcggaaaa
    85021 atcagtgaaa gcccaggaga caccgtactt acgccgctca ttttgatacc agcgaacata
    85081 agcctgtgcg ctgttgcctg taacaccatt tttcattgcc cgtaatggaa cgcggtgaga
    85141 gaggcgttcc agttgcgaac caatacgcca gttatcatta aaatcatacc agccagacag
    85201 gcgcgcgccg ggtttatgct catgattgaa aaagcgttca gcgtactccg cctcgagcca
    85261 gatattacgt gaccgccact caacacctgc cagccagtcg cgaacaatcc cttttccttc
    85321 gctaaattgt ccatcggcat aaccgaatcc agcaaaaccg cgccagttat cattcagcgg
    85381 tggtgaataa acgatggtgg ttaagtcgac atcatgtttg ccactatctg gaccttcggc
    85441 atcaattcct gttgagccag cgatacgaag ctctgcaaga ttatgtacat caaccgcacg
    85501 ttttaatcgt acaacgccgg gatcttgcgg ttcacgttcg acaacatcgt gggttaagac
    85561 agctgcctgc tgccattctt gtaacgttaa tgctgtccag gcttgttcaa cctccagatt
    85621 aatattacgt ggctcgatca cttctgcttt ttttaattca ttttctgccg cacgaggcca
    85681 accgcgggct tgtaacacac tcgcgtaatc gatgcgcagt ccctgatttc ctggtgcgtt
    85741 ataagcaagt tctctggttg ttatttcagc ttgaggaaga tcattactat attttgctac
    85801 ggttgagaga aacgaatgcc cctgtaacca ggtatcattc gggatgctcg taggcgtgcc
    85861 cattagccga aggaaaggcg gcgaagtatt aatggtatgt ttggtgacag ttagcgcgcc
    85921 gggataattt tcactctcca ggtggctgta aaagagatcc gcaagttctt catcggataa
    85981 atccgtggca atggtttcct tgttataaaa gagctcggtc attattgact gtgctttttt
    86041 cggctgctga tctttgagat aagccgacgc aacccaatat agcgcccagg gcggaataat
    86101 ttgccccgtc ttttttaaac gctgatagtg agaaataacg tctctataac gatcgcgagt
    86161 taataacgcg ccaagatgat caacctgaat acgctggtac tgggcagtgc ggtctgggtt
    86221 atcgtgccac agaatttcta atgcagcgta ttgggcgagg gcgcgatcgg caatggcata
    86281 acgttcattt tcactgcgcg taggcataaa cgacagtctg accagttcgg catgaatatc
    86341 agcgcgaata tctggcgtta aattggcatc gtcaatcgcg gcagctagtt gattattacg
    86401 taatgccagc acgtattctg tgggatattg ttgcttagat gcattttcag gtaatgactc
    86461 tgtcatcgcc cgtaattcat cctgataacg ccccgtcagt ttatagatat aggcttctgc
    86521 gagtaaattg gctttgtccg gtgctccaga tttaagctgc ttaagtttaa acagcgcagt
    86581 atcatagtga cccgcatctg ccagggttaa aatttgtccc cgttgataat ccttattttg
    86641 cggctccaga gagagcgcct tttgccacag tgtaagcgag ttttgccatt gttgcaggtt
    86701 acgataagcg acggcgacag ctgcataacc acgcgctggt aattgctgat gacgatagcg
    86761 gttgtaaacg gtaataacct gtttatcttg cccggcccat aaggcaatct gtaaccagtc
    86821 agcaatttgg ttattactga gtgttgattt ttgtgcaaac catgacaaag ctggctgagt
    86881 atttcctttg cgagcttcaa taatcaatgc atcataggcg ttattaacag cactcttcgc
    86941 cgcgggactc gctgctaaaa atgcagcagt aagaattttc aaagcccatt tggttttcgg
    87001 gcaccttttt ctgctacttg aatacatcct gtattactcc atgtattgcc aaaatctctc
    87061 tctgtatcta attacaggta actgaaaaga aagatatttt tgcatctcat aatccgttat
    87121 taaacgcgga agagagacgt gaat
//
LOCUS       DACADF010000003        75218 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.003, whole genome shotgun sequence.
ACCESSION   DACADF010000003 DACADF010000000
VERSION     DACADF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.003"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..1554
                     /locus_tag="ISB30_000179"
     CDS             <1..1554
                     /locus_tag="ISB30_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP1987865.1"
                     /translation="ALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVF
                     RRQFSEMTDLVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERANKL
                     LSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQ
                     RIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIATVNDFDSSFGYRNLRIHKSAIC
                     EPLQGENAWSYAEKLKLAISSIRNHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIG
                     ELFLVYQPIVDINTRTILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIK
                     TAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEIT
                     ETVIERINEHFYLNIEQLRKQGVRVSIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDI
                     FGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAW
                     DKSGKLVKE"
     gene            1562..2890
                     /locus_tag="ISB30_000180"
     CDS             1562..2890
                     /locus_tag="ISB30_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP1987866.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(2937..4262)
                     /gene="rimO"
                     /locus_tag="ISB30_000181"
     CDS             complement(2937..4262)
                     /gene="rimO"
                     /locus_tag="ISB30_000181"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAP1987867.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            4475..4858
                     /gene="bssR"
                     /locus_tag="ISB30_000182"
     CDS             4475..4858
                     /gene="bssR"
                     /locus_tag="ISB30_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAP1987868.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            4969..6084
                     /locus_tag="ISB30_000183"
     CDS             4969..6084
                     /locus_tag="ISB30_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAP1987869.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(6081..6707)
                     /locus_tag="ISB30_000184"
     CDS             complement(6081..6707)
                     /locus_tag="ISB30_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAP1987870.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDTDF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            6954..8156
                     /gene="dacC"
                     /locus_tag="ISB30_000185"
     CDS             6954..8156
                     /gene="dacC"
                     /locus_tag="ISB30_000185"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAP1987871.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(8203..8961)
                     /gene="deoR"
                     /locus_tag="ISB30_000186"
     CDS             complement(8203..8961)
                     /gene="deoR"
                     /locus_tag="ISB30_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAP1987872.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHISKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(9019..9615)
                     /gene="ybjG"
                     /locus_tag="ISB30_000187"
     CDS             complement(9019..9615)
                     /gene="ybjG"
                     /locus_tag="ISB30_000187"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP1987873.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            9900..11132
                     /gene="mdfA"
                     /locus_tag="ISB30_000188"
     CDS             9900..11132
                     /gene="mdfA"
                     /locus_tag="ISB30_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAP1987874.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(11173..11457)
                     /locus_tag="ISB30_000189"
     CDS             complement(11173..11457)
                     /locus_tag="ISB30_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987875.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(11543..12358)
                     /gene="ybjI"
                     /locus_tag="ISB30_000190"
     CDS             complement(11543..12358)
                     /gene="ybjI"
                     /locus_tag="ISB30_000190"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAP1987876.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ"
     gene            complement(12358..13566)
                     /locus_tag="ISB30_000191"
     CDS             complement(12358..13566)
                     /locus_tag="ISB30_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1987877.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMVLSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATMHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMMVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            13650..14186
                     /locus_tag="ISB30_000192"
     CDS             13650..14186
                     /locus_tag="ISB30_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1987878.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            complement(14362..16047)
                     /locus_tag="ISB30_000193"
     CDS             complement(14362..16047)
                     /locus_tag="ISB30_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAP1987879.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            16316..16693
                     /locus_tag="ISB30_000194"
     CDS             16316..16693
                     /locus_tag="ISB30_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987880.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(16723..16980)
                     /gene="grxA"
                     /locus_tag="ISB30_000195"
     CDS             complement(16723..16980)
                     /gene="grxA"
                     /locus_tag="ISB30_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAP1987881.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            17140..17427
                     /locus_tag="ISB30_000196"
     CDS             17140..17427
                     /locus_tag="ISB30_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAP1987882.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            17411..18133
                     /gene="nfsA"
                     /locus_tag="ISB30_000197"
     CDS             17411..18133
                     /gene="nfsA"
                     /locus_tag="ISB30_000197"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAP1987883.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            18194..19096
                     /gene="rimK"
                     /locus_tag="ISB30_000198"
     CDS             18194..19096
                     /gene="rimK"
                     /locus_tag="ISB30_000198"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAP1987884.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            19184..19660
                     /locus_tag="ISB30_000199"
     CDS             19184..19660
                     /locus_tag="ISB30_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAP1987885.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            20010..21122
                     /gene="potF"
                     /locus_tag="ISB30_000200"
     CDS             20010..21122
                     /gene="potF"
                     /locus_tag="ISB30_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAP1987886.1"
                     /translation="MIALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            21217..22350
                     /gene="potG"
                     /locus_tag="ISB30_000201"
     CDS             21217..22350
                     /gene="potG"
                     /locus_tag="ISB30_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAP1987887.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            22360..23304
                     /gene="potH"
                     /locus_tag="ISB30_000202"
     CDS             22360..23304
                     /gene="potH"
                     /locus_tag="ISB30_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAP1987888.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGIIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIVMLLLLIMPIMWFHKHQQKSEGEHG"
     gene            23301..24146
                     /gene="potI"
                     /locus_tag="ISB30_000203"
     CDS             23301..24146
                     /gene="potI"
                     /locus_tag="ISB30_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAP1987889.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVALSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            24206..24694
                     /locus_tag="ISB30_000204"
     CDS             24206..24694
                     /locus_tag="ISB30_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAP1987890.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            24735..25862
                     /gene="rlmC"
                     /locus_tag="ISB30_000205"
     CDS             24735..25862
                     /gene="rlmC"
                     /locus_tag="ISB30_000205"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAP1987891.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(25891..26622)
                     /gene="artJ"
                     /locus_tag="ISB30_000206"
     CDS             complement(25891..26622)
                     /gene="artJ"
                     /locus_tag="ISB30_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1987892.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(26848..27516)
                     /gene="artM"
                     /locus_tag="ISB30_000207"
     CDS             complement(26848..27516)
                     /gene="artM"
                     /locus_tag="ISB30_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAP1987893.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(27516..28232)
                     /gene="artQ"
                     /locus_tag="ISB30_000208"
     CDS             complement(27516..28232)
                     /gene="artQ"
                     /locus_tag="ISB30_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAP1987894.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(28239..28970)
                     /gene="artJ"
                     /locus_tag="ISB30_000209"
     CDS             complement(28239..28970)
                     /gene="artJ"
                     /locus_tag="ISB30_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1987895.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KFNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(28988..29716)
                     /gene="artP"
                     /locus_tag="ISB30_000210"
     CDS             complement(28988..29716)
                     /gene="artP"
                     /locus_tag="ISB30_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAP1987896.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(29934..30449)
                     /locus_tag="ISB30_000211"
     CDS             complement(29934..30449)
                     /locus_tag="ISB30_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP1987897.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            30575..30898
                     /locus_tag="ISB30_000212"
     CDS             30575..30898
                     /locus_tag="ISB30_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP1987898.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            30895..31725
                     /locus_tag="ISB30_000213"
     CDS             30895..31725
                     /locus_tag="ISB30_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP1987899.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(31722..32735)
                     /locus_tag="ISB30_000214"
     CDS             complement(31722..32735)
                     /locus_tag="ISB30_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP1987900.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(32834..34264)
                     /locus_tag="ISB30_000215"
     CDS             complement(32834..34264)
                     /locus_tag="ISB30_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1987901.1"
                     /translation="MPQRILVLGASGYIGQHLVHTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLNWPDNLPVLLQNIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQVIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPACEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLLPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQPLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLQALWRVVNQIGGKERYFFGNILWQTRALMDLAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(34275..35276)
                     /gene="ltaE"
                     /locus_tag="ISB30_000216"
     CDS             complement(34275..35276)
                     /gene="ltaE"
                     /locus_tag="ISB30_000216"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAP1987902.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(35313..37031)
                     /gene="poxB"
                     /locus_tag="ISB30_000217"
     CDS             complement(35313..37031)
                     /gene="poxB"
                     /locus_tag="ISB30_000217"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAP1987903.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGNGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRVFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTERKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(37164..38132)
                     /gene="hcr"
                     /locus_tag="ISB30_000218"
     CDS             complement(37164..38132)
                     /gene="hcr"
                     /locus_tag="ISB30_000218"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAP1987904.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKTLG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVFA"
     gene            complement(38144..39796)
                     /gene="hcp"
                     /locus_tag="ISB30_000219"
                     /gene_synonym="priS"
     CDS             complement(38144..39796)
                     /gene="hcp"
                     /locus_tag="ISB30_000219"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAP1987905.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIATLQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIIGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(39940..40839)
                     /gene="lysO"
                     /locus_tag="ISB30_000220"
     CDS             complement(39940..40839)
                     /gene="lysO"
                     /locus_tag="ISB30_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAP1987906.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSTTVILLCNIAALMWLERGLPWSNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(41254..41949)
                     /gene="aqpZ"
                     /locus_tag="ISB30_000221"
     CDS             complement(41254..41949)
                     /gene="aqpZ"
                     /locus_tag="ISB30_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAP1987907.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            42374..44032
                     /locus_tag="ISB30_000222"
     CDS             42374..44032
                     /locus_tag="ISB30_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAP1987908.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENIPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(44029..44985)
                     /locus_tag="ISB30_000223"
     CDS             complement(44029..44985)
                     /locus_tag="ISB30_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAP1987909.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            45136..46251
                     /gene="macA"
                     /locus_tag="ISB30_000224"
     CDS             45136..46251
                     /gene="macA"
                     /locus_tag="ISB30_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAP1987910.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            46248..48194
                     /gene="macB"
                     /locus_tag="ISB30_000225"
     CDS             46248..48194
                     /gene="macB"
                     /locus_tag="ISB30_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAP1987911.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLEWKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(48267..48491)
                     /gene="cspD"
                     /locus_tag="ISB30_000226"
     CDS             complement(48267..48491)
                     /gene="cspD"
                     /locus_tag="ISB30_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAP1987912.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            48814..49134
                     /gene="clpS"
                     /locus_tag="ISB30_000227"
     CDS             48814..49134
                     /gene="clpS"
                     /locus_tag="ISB30_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAP1987913.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            49165..51441
                     /gene="clpA"
                     /locus_tag="ISB30_000228"
     CDS             49165..51441
                     /gene="clpA"
                     /locus_tag="ISB30_000228"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAP1987914.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(51785..51872)
                     /locus_tag="ISB30_000229"
     tRNA            complement(51785..51872)
                     /locus_tag="ISB30_000229"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51836..51838),aa:Ser,seq:gga)
     gene            complement(52126..52344)
                     /gene="infA"
                     /locus_tag="ISB30_000230"
     CDS             complement(52126..52344)
                     /gene="infA"
                     /locus_tag="ISB30_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP1987915.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(52629..53333)
                     /gene="aat"
                     /locus_tag="ISB30_000231"
     CDS             complement(52629..53333)
                     /gene="aat"
                     /locus_tag="ISB30_000231"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAP1987916.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LSNNFWVPRCLFSPQE"
     gene            complement(53375..55096)
                     /gene="cydC"
                     /locus_tag="ISB30_000232"
     CDS             complement(53375..55096)
                     /gene="cydC"
                     /locus_tag="ISB30_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAP1987917.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPKVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(55097..56863)
                     /gene="cydD"
                     /locus_tag="ISB30_000233"
     CDS             complement(55097..56863)
                     /gene="cydD"
                     /locus_tag="ISB30_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAP1987918.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(56986..57951)
                     /gene="trxB"
                     /locus_tag="ISB30_000234"
     CDS             complement(56986..57951)
                     /gene="trxB"
                     /locus_tag="ISB30_000234"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP1987919.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNTDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            58495..58989
                     /gene="lrp"
                     /locus_tag="ISB30_000235"
     CDS             58495..58989
                     /gene="lrp"
                     /locus_tag="ISB30_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAP1987920.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            59124..63230
                     /gene="ftsK"
                     /locus_tag="ISB30_000236"
     CDS             59124..63230
                     /gene="ftsK"
                     /locus_tag="ISB30_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP1987921.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPAQSTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAAEQPAQQPYYAPA
                     PEQAVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPTRPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAQQPQYQQPQQPVTQQPQYQQPQQPVVPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            63389..64000
                     /gene="lolA"
                     /locus_tag="ISB30_000237"
     CDS             63389..64000
                     /gene="lolA"
                     /locus_tag="ISB30_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAP1987922.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            64011..65354
                     /locus_tag="ISB30_000238"
     CDS             64011..65354
                     /locus_tag="ISB30_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP1987923.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAVGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            65445..66737
                     /gene="serS"
                     /locus_tag="ISB30_000239"
     CDS             65445..66737
                     /gene="serS"
                     /locus_tag="ISB30_000239"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP1987924.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(67043..67183)
                     /locus_tag="ISB30_000240"
     CDS             complement(67043..67183)
                     /locus_tag="ISB30_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HAP1987925.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(67375..67635)
                     /locus_tag="ISB30_000241"
     CDS             complement(67375..67635)
                     /locus_tag="ISB30_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HAP1987926.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHDLPASHRGNNQMSLPLSQN"
     gene            complement(67676..68785)
                     /locus_tag="ISB30_000242"
     CDS             complement(67676..68785)
                     /locus_tag="ISB30_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HAP1987927.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            68943..70127
                     /locus_tag="ISB30_000243"
     CDS             68943..70127
                     /locus_tag="ISB30_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HAP1987928.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGRTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNGTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEFFGPAFAVLG
                     GA"
     gene            70127..70639
                     /locus_tag="ISB30_000244"
     CDS             70127..70639
                     /locus_tag="ISB30_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HAP1987929.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTPPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRALFLKYGGTIDGTLLRFAGEYYTDAESDLYEIE
                     MRGRVTEIDMGEAKQGEATSHTYAVKNTYYKLSVNDRPLWEIDLLNHIYRKDGKDIVP
                     DRIRSALGLG"
     gene            70695..71069
                     /locus_tag="ISB30_000245"
     CDS             70695..71069
                     /locus_tag="ISB30_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1987930.1"
                     /translation="MKDIDTETRNNTVADDVTSGEDMAVERGVKLSRPIERGAEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            71078..71233
                     /locus_tag="ISB30_000246"
     CDS             71078..71233
                     /locus_tag="ISB30_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HAP1987931.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            71220..74027
                     /locus_tag="ISB30_000247"
     CDS             71220..74027
                     /locus_tag="ISB30_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP1987932.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     QARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTASDAASGQSFLAMAGFTPQAIQAALPGVLNMALAG
                     GVELGETADIGSNILTQFNLTADQMDRVGDTLTAAFTRTNTDLRALGETMKYTGPVAA
                     KLGISLEEAAAMAGMLANNGLRGSDAGTAMRASLSRLASPPKAAADALKELGVSVADA
                     RGKMRPMEDVLLDLYKATQKYGQVDQVSFFKDIAGEEAFVGLQTLVAAAGSGELQKLT
                     RELQGARGEADRVAKVMADNLDGDLKNLDSAWEGLRIRISDLVDGPLRSVTQWLTRVL
                     EKITSLAQAHPVLTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRLLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            74040..74528
                     /locus_tag="ISB30_000248"
     CDS             74040..74528
                     /locus_tag="ISB30_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1987933.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(74557..75156)
                     /locus_tag="ISB30_000249"
     CDS             complement(74557..75156)
                     /locus_tag="ISB30_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP1987934.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGTSTIYRYHPVGDIQTEETTGQTQENENR"
BASE COUNT        18435 a        18676 c        19984 g        18123 t
ORIGIN      
        1 gcattattcg aaatctattt ttattatcct ggtggtaatt tattgaacgc atcagataaa
       61 cttttttatc tgccgtttgc tttcattatt atcgtattgc tggtggttta tttaatgacc
      121 actcgagttt tccgtcgtca attttctgaa atgacagatt tggtcaatac gctggcgttt
      181 ttgcctgact ctacggatca aatcgaggcc ctgaagattc gtgaaggcga tgcgaaagag
      241 attatcagca tcaaaaattc gatcgcggaa atgaaagatg ctgaaattga acgggcaaat
      301 aaattgctct cgctgatctc ttacgatcag gaaagcggtt ttattaaaaa tatggcgatt
      361 attgagtcca acaataatca gtatctggct gtggggatca tcaaactgtg tggtctggaa
      421 gctgtggaag cggtgtttgg tgttgatgaa cgcaataaaa tcgtcaggaa attgtgtcag
      481 cgaattgccg agaaatatgc gcaatgctgc gatatcgtga catttaatgc cgatctctat
      541 ttactcctgt gccgggaaaa tgtacagacg tttacccgta agatagcgac ggtaaacgat
      601 tttgacagca gttttggcta ccgcaatctg cgcatccata agtctgctat ttgtgaacct
      661 ttgcaggggg aaaacgcctg gagttacgca gaaaagctga aactggcgat ttccagtatc
      721 cgcaaccata tgttctcaga gtttattttc tgtgatgatg cgaaactcaa cgaaatagaa
      781 gagaatatct ggattgcgcg taatattcgc catgcaatgg aaattggcga actattcctg
      841 gtctatcaac cgattgttga tattaacacc cgcaccattt tgggcgcgga ggcgttgtgc
      901 cgttgggtgt ctgcggagcg ggggattatt tcaccgctga agttcattac cattgctgaa
      961 gatatcgggt ttatcaatga gctgggctat caaattatta aaaccgcgat gggtgaattc
     1021 agacatttta gtcagcgtgc gtcgctgaag gatgatttct tactgcatat taatgtttcg
     1081 ccctggcagt taaacgaacc acactttcat gagcgtttta ctaccatcat gaaagaaaat
     1141 ggcctgaagg cgaacagcct ttgtgttgag atcaccgaaa ccgtgatcga gcgaattaat
     1201 gaacatttct atctcaatat tgaacaactg cgtaaacaag gggtacgggt atcgattgat
     1261 gactttggca ccggtttgtc aaacctgaaa cgtttttatg aaattaatcc agacagtata
     1321 aaggtggatt cgcaatttac cggcgatatt ttcggtactg cgggaaaaat tgtgcgcatt
     1381 attttcgatc tggcacgcta taaccggatc ccggtgattg cggaaggcgt agagagcgaa
     1441 gacgttgcgc gtgaattaat caaattagga tgtgttcagg ctcaggggta tctgtaccag
     1501 aaacccatgc cattctccgc ctgggataaa agtggaaaat tagtaaaaga gtagtttacg
     1561 tatgtccaga atcaataaat tcgtacttac agtcagtctg ctgattttta tcatgatttc
     1621 agcagttgcc tgcgggatct acactcaaat ggtaaaggaa cgggtgtatg gcctgaaaca
     1681 gtccgttatt gatactgctt ttgcggtggc aaatattgct gaatatcggc ggagtgtggc
     1741 aattgatctg atcaacacgc taaatcctac ggaggaacag ctgttggttg gcttacgcac
     1801 agcttacgcc gactcggttt caccctctta tttgtacgat gtcggtcctt atctgatttc
     1861 cagtgacgaa tgtattcagg taaaggagtt tgagaaaaat tattgtgcag atattatgca
     1921 ggttgttaag tatcgacatg tcaaaaatac agggtttatc tcttttgatg gtaaaacctt
     1981 cgtctattac ctctatccgg taactcacaa tcgtagtctg atatttttgc ttggtctgga
     2041 acgtttttct ttactgtcaa aatcgctggc gatggatagc gagaacctga tgttctccct
     2101 gttcaagaac ggtaaatcgg tgaccggtga tgaatataac gctaaaaacg ccatcttcac
     2161 cgtttcggaa gcaatggagc acttctccta tttgccgacc ggattgtacg tatttgctta
     2221 taaaaaagac gtttattttc aggtatgtac attgattatt ttctttgccg cattggtggc
     2281 agtgatatcg ggtgccagtt gcctctatct ggtacgcaga gtgattaatc gtggtattgt
     2341 ggagaaagaa gccattatta ataaccattt tgaacgcgta ctggatggcg ggcttttctt
     2401 ttcggctgcc gatgtcaaaa aactctacag tatgtataac tcggcattcc tggacgacct
     2461 gaccaaagcg atgggcagaa aatcctttga cgaagattta aaagcgctgc cggaaaaagg
     2521 cggttatttg tgcctgtttg acgtcgataa attcaaaaac attaacgaca ccttcggtca
     2581 tttgctgggc gatgaagtgt tgatgaaagt ggtgaaaatc cttaaatcac agatcccggt
     2641 agataaaggt aaagtctacc gcttcggagg tgacgaattt gcggtgattt atactggcgg
     2701 cacgctggaa gagttgctat cgattctaaa agaaatcgtt catttccagg tgggaagcat
     2761 taatttaagc accagtatcg gtgtagcgca ttcaaatgaa tgtactaccg tcgaacgatt
     2821 gaaaatgctg gcggatgagc ggctgtataa gagtaagaaa aacggcaggg cgcagattag
     2881 ctggcagtaa tcattattcg caggccggac aaatgatttt gcccggcctg aattaattaa
     2941 acccggctac cccacaaatc gtactcatcg gcgtgctcga ctttcactcg caggatatca
     3001 cccggcttaa cgttggtttc accattgaga taaaccgcgc cgtcgatttc cggtgcatct
     3061 gccatgctgc gaccaatcgc gccttcttcg tccacttcgt cgataatcac cagaatttca
     3121 cgacccactt tctcttgcag gcgctcggcg gaaatctgct gctgcagctg catgaaacgg
     3181 ttccagcgtt cttctttcac ttcttccgga acctggtcag gcagggcatt ggcgtctgca
     3241 ccttcaaccg ggctgtattt aaagcagcca acgcgatcca gacgtgcttc tttcaggaag
     3301 tcgagtagca tctggaaatc ttcttccgtc tcgccaggga agccaacaat aaaggttgag
     3361 cgtagggtca gttccgggca gatttcgcgc cactgtttga tgcgcgccag ttggcgatct
     3421 acagaacccg gacgcttcat cagtttgaga atgcgcgggc tggcgtgctg caacggaatg
     3481 tccagatacg gcaggatttt gccctctgcc atcagtggga tgacgtcgtc cacatgcgga
     3541 taagggtaaa cgtagtgcag acgtgtccag atccccagtt tcgataactg ttcgcacagg
     3601 ctgaccatgc tggtttttac cggctcgccg ttgtggaagc cagtacgatg tttaacatcg
     3661 acgccatacg cggaagtatc ctgcgagatc accagaattt ctttaacgcc cgcatcaacc
     3721 agacgtttcg cttcacttaa cacttcgcca atcggacggc tcaccagatc gccgcgcata
     3781 gacggaataa tgcagaaggt gcagcggtga ttacagcctt cagaaatttt cagataagca
     3841 taatgacgcg gcgtcagttt cacaccttgt tctggcacca ggctcaggaa cgggttgtgt
     3901 ttcggttttg gcacgtagtg atgaacgtgc tccagaacct gctcatagct atgaggcccg
     3961 gtgatttcca gcactttcgg gtggacttcg cggatctgat cttcttttgc ccccagacaa
     4021 ccggtcacaa ttaccttgcc gttttcattc aacgcttcac caatggcttc cagtgattct
     4081 tgtaccgcgc tgtcaataaa gccgcaggtg ttgacgatca ccatgtccgc atcgtcatag
     4141 ctcggtacca cgtcataacc ttcagtgcgt agttcggtga gaatacgctc tgaatcgaca
     4201 aggtttttcg ggcagccaag ggaaacaaag ccgattttcg gctggggagt tactttgctc
     4261 atagcttaaa aaatattcag ttacaggaaa ggtcagggca gggattctac agagttctgg
     4321 ataaaatttg tatcgcaatc tcattcgctg gcggagttga aggaaatgta aattttgtta
     4381 attcggcgtg aagaattgat cctggacagc attttgctca aaaaacagcc atactattta
     4441 attgcaacaa ggctggaaag aggaggatcg aagtatgttc gttgacagac agcgaatcga
     4501 cctgctgaac cggttgatcg acgcacgcgt tgacctcgcc gcatacgtgc aactgaggaa
     4561 ggcaaaagga tacatgtccg tcagcgaaag caatcatcta cgagataact tttttaaact
     4621 gaatcgcgaa ctgcacgata aatcgctgcg gttgaatctt catctggatc aggaagagtg
     4681 gagtgctctt catcatgctg aagaagcatt agcgacagcc gcagtatgtt tgatgagtgg
     4741 gcaccatgat tgcccgactg ttattaccgt caacgccgat aagcttgaaa attgtctgat
     4801 gagcttaacg ctgagtatcc agagcctgca gaagcacgcc atgcttgaga aggcctgaaa
     4861 actaaggggg agaaagcgtc tcccccttca tgtttaagtt tttgtaaaaa tgaatttgtt
     4921 ctctcctcca ctgactacgc tttaagccag agtcaatccg gaggcgttat gcatcgacaa
     4981 tcctttttcc ttgtgcccct tatttgtctt tcttccgctc tctgggcggc tcctgcaacg
     5041 gtaaatgtcg aagtactgca agacaaactc gaccatccct gggcgctggc ctttttaccc
     5101 gataatcacg gtatgttaat cactctgcgc ggcggcgagt tgcgtcactg gcaagcagga
     5161 aaaggattat ctgcgccgct ttccggagtg ccggacgttt gggcgcacgg gcagggcggc
     5221 ctgctggacg tggttttagc gcccgatttt gctcagtcgc gccgcatctg gttaagttat
     5281 tccgaagttg gcgatgatgg caaagccgga actgctgtgg gttatggtcg cttaagtgat
     5341 gatctctcaa aagtgaccga cttccgcacc gtctttcgtc agatgccaaa actgtctacc
     5401 ggcaaccatt ttggcgggcg gatggtattt gacggtaaag gttatctttt tattgctctg
     5461 ggcgaaaaca atcagcgccc gacggcgcag gatctggata aattacaggg caaactggtg
     5521 cgtctgaccg acctgggaga aatcccggat gataatcctt ttataaagga atccggtgtg
     5581 cgcgccgaga tctggtctta tggcattcgt aatccgcaag gaatggcgat gaatccgtgg
     5641 agtaatgcac tatggctgaa tgaacatggc ccgcgcggtg gtgatgaaat taatattccg
     5701 caaaaaggca aaaactacgg ctggccgctg gcaacctggg gaatcaacta ttcgggcttt
     5761 aagataccgg aagcgaaagg ggagatcgtc gccgggaccg agcaacctgt tttttactgg
     5821 aaagattcgc ccgccgtgag cggcatggct ttctataaca gcgacaaatt cccccagtgg
     5881 cagcaaaaat tatttattgg cgcgctgaaa gataaagatg tcattgtgat gagcgtcaac
     5941 ggcgacaaag tgacagaaga tggccgtatt ttaacggaca gagggcaacg aattcgtgat
     6001 gttcgcactg gacccgacgg ttatttatac gttctcaccg acgagtccag tggggaatta
     6061 cttaaagtta gcccacgcaa ttagctaacg ggaatcatca ccactttgcg cactgcgggg
     6121 cgttcagtga gttgctgata ccagcgttgc agatttggac gcggtgtcca ggtcaggcca
     6181 acgttaaaca aattgtagat gaacggtgcg atagcgatat cgcccacacc aaactcgtcg
     6241 cctgagaacc attttacttt tgccaattcc gcatcgagca gggcaaacag ggcgtcgcac
     6301 tctttgcaac tggcatcaat ggcggcctga tcgcgctctt ccggtggtgt tctgactaat
     6361 cccatcagga tcccgcgatg agcattactg agcgtctggt ttgcccagtc catccatttt
     6421 tctgcttccg cacgacgtgc cggtgagtcg atccacaggc gtttttgccc gtactgtgcc
     6481 gccagatagc ggacaatggc gtttgattcc caaagaatga gatcactttc gtcgtcacgc
     6541 aacaacggca ccagcccgtt agggttcatc gccagaaaat cagtatcgtg atttatccca
     6601 aactcacggc ccgcgagaat ttgctcataa ggtagttcca gttcttcgag cgtcagcaat
     6661 acttttttta cgttggttga attattccga ccccacagcg taatcatact cacccctttt
     6721 ccgacaggca gccacaggct aaacaggact ccgatggtga agtaaagtta acttttaagc
     6781 aacagctggc aaaaaaatga caccagaagc agcgaagcgg caggatattg cataaacttt
     6841 aaaaacttta ccaacttacg gtttctttaa gtttgtgtgt gcgttattaa tcaccgaact
     6901 tatcatacgg cgatataacg tatttttttt gaatggatac tcgggtggca tttatgacgc
     6961 aatactcctc tctccttcgt ggtcttgcag cgggttctgc atttttattc ctttttgccc
     7021 caacggcatt cgcggcggaa caaaccgttg aagcgccgag cgtggatgcc cgtgcatgga
     7081 ttttaatgga ttacgccagc ggtaaagtgc tggcagaagg caatgcggat gagaaactgg
     7141 atcccgcgag cctgactaaa attatgacca gctatgtggt tgggcaggcg cttaaggccg
     7201 ataaaattaa actcaccgat atggtgacgg tcggtaaaga tgcctgggcg acgggaaatc
     7261 cggcactgcg tggttcatcg gtaatgttcc tcaaaccggg cgatcaggtt tcggtggcag
     7321 acttgaacaa aggtgtgatt atccagtccg gtaatgacgc ctgtattgcg ctggctgatt
     7381 acgttgccgg gagccaggag tcatttattg gtttgatgaa tggttatgcc aaaaaactgg
     7441 gtctgaccaa cactaccttc cagacggtgc acggcctgga tgcgccgggg cagttcagta
     7501 ccgcgcgcga tatggcattg ctgggtaaag cattgatcca cgatgtgccg gaagagtacg
     7561 ccattcataa agagaaagag ttcaccttca acaaaattcg tcagcctaac cgtaatcgtc
     7621 tgctgtggag cagcaatctg aatgttgatg gcatgaagac aggaaccacg gcaggcgcgg
     7681 gatataatct ggttgcttcg gctacccagg gcgatatgcg tttaatctcc gtagtgctgg
     7741 gggcgaaaac tgaccgtatc cgttttaatg agtctgagaa attattgacc tggggtttcc
     7801 gcttctttga aaccgtgacg ccaattaaac ctgatgccac atttgtgact cagcgcgtct
     7861 ggtttggtga taagagcgaa gtgaatctcg gggcaggcga agcgggctca gtgaccatac
     7921 cgcgtgggca gctgaaaaac ctgaaggcga gttatacgtt aacggaaccg cagcttaccg
     7981 caccgctgaa aaaaggtcag gttgtcggca ccattgattt ccagcttaac ggtaaatcca
     8041 ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc tttggtcgga
     8101 tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc tcttaatctt
     8161 ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca tcaacttaat
     8221 acgctgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcgc tcacaacaat
     8281 atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt tactgtggtc
     8341 gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg gcaactcttc
     8401 aagattaaaa caggtagcgc ctttactgat atgcacgccc gcagcggaat aaaaagcgat
     8461 atccgggcaa aaattattca gtgtttgctg aaaatcgatg ggtttgaaaa tggcgttgct
     8521 ggcgtgaaat tcaccaccgc aaagaaacgc gcggcaatgg ggtttctctt tcagcgccag
     8581 aaaagtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa ttgcttcaat
     8641 aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt ctaccagcgt
     8701 cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt gatcgcttaa
     8761 caggtaatgg ctggcgctgc gtggttccag aacaatatag ccgccgagca aaacgacggg
     8821 cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa gcagggcggc
     8881 ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct gcccgatacg
     8941 ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc cgctcaatac
     9001 tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta atgcaaaaca
     9061 gaagcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt gccagataat
     9121 ctgggcgcta aggcagccaa tcatacctgc cagcaatcca ccgagcatat ccagcggcca
     9181 gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca ttaaaagtga
     9241 gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa tcaccgtacc
     9301 gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc cgatattttc
     9361 gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa acaggctgac
     9421 cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc cccacaacca
     9481 aagtactgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa aaatcgccaa
     9541 cgagatcatc cacggcgcag agtctggcgt tgcgttaata agagagaata gagagagatt
     9601 caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa tacaggcaag
     9661 tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat ttccctcaag
     9721 cggcctgttt acggtaggtg attgtaacgg gcaaaggtta aataaaactt aaagaaagcg
     9781 tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta caaaattcgc
     9841 tttctcgtta gctgcgcttt tattaaactt tgcgcgatta ttattggcga agaaattgca
     9901 tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt ttccctctct
     9961 gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgattcaa cccggtatgt
    10021 tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg atgactgcgt
    10081 atctggcggg cgggatgttt ttacaatggc tcctggggcc gttgtcggat cgtattggcc
    10141 gccgtccggt gatgctggcg ggagtggtgt ggtttatcgt cacctgtctg gcaatattac
    10201 tggcacaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggtata agcctctgtt
    10261 tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg gtttgtatca
    10321 agatcaccgc gctaatggcg aacgtggcgc tgattgctcc actacttggt ccgctggtgg
    10381 gcgcggcgtg gatccatgtg ctgccctggg aagggatgtt tgtcttgttt gccgcattgg
    10441 cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccacg cgtataggcg
    10501 agaaactgtc gctgaaagaa ctcggtcgtg actataagct ggtgctgaag aacggtcgct
    10561 ttgtggcggg ggcgctggcg ctgggattcg ttagcctgcc gttgctggcg tggatcgccc
    10621 agtcgccgat tatcattatt accggtgagc agttgagcag ctatgaatat ggcctgctgc
    10681 aagtgcctat ttttggggcg ttaattgcgg gtaacttgct gttagcgcgt ctgacctcgc
    10741 gccgcaccgt acgttcgctg attattatgg gcggctggcc gattatgatt ggtctgttgg
    10801 tcgctgctgc ggcaacggtt atctcatcgc acgcgtattt atggatgacc gccgggttaa
    10861 gtatttatgc tttcggtatt ggtctggcga atgcgggact ggtgcgatta accctgtttg
    10921 ccagcgatat gagtaaaggt acggtttctg cggcgatggg gatgctgcaa atgctgatct
    10981 ttaccgtcgg tattgaaatc agcaaacatg cctggctgaa cgggggcaac ggactgttta
    11041 atctcttcaa ccttgtcaac ggcattttgt ggctgtcgct gatggttatc tttttaaaag
    11101 ataaacagat gggaaattcg cacgaagggt aaaaaaatgc ctgattgctt tgtgcgatca
    11161 ggcattgtcg aattaatggt gatggtcgtc aatctggtgt tcgataacca tcccttcacc
    11221 tacgctggca agatggcgaa cataaggatg cgggcggtaa gccggagctg gcgcaggagc
    11281 cacatagacg gtttgcggca cagtcgcgac gctgaccgct tgtggaacgc tgactgaatc
    11341 aggaaccaca accactttgt atccactcgg cacatcgacg gtgacacttt gcgccatcgc
    11401 cacaccagta aagcccatta aaagcgcgcc caacagtaga caatttttca taatcatttc
    11461 gcctgaaata atgcagcggg ttaatttccg cgaattatgc agccttcatt caggtattgt
    11521 gttgccgggc gtgctgcctt ttttattgct taaatggcgc ttcgtgttta agcactttat
    11581 cgatcacatc cagtacgcct tcacggttat tggagcctgc ccggtatttt gccgctgcga
    11641 cgaccgcgct gccggcattt tccattgcaa agctaaaccc tgcctgacgc agcatctcaa
    11701 tatcgttacc gccatcgcca aagaccacca cttcgctgtc gtctattccc cataatttct
    11761 gcagttggcg aaggccattg gctttatgta caccggggat aatcagatcg atgctgccgt
    11821 tgccggtatg gaccggtacc atgatatcgc cgatggcctc atgtaatgct ttttgcactt
    11881 gtgggatcag ttcgtcagag agattaaggc caaatttaaa gaagatatct tctaagttgt
    11941 caaagttatc gacgtattcc agacggtgat aatacatttc cgccaccgtt ttcatggcat
    12001 cgtcatattt tttgagggta taggcgctat tttttccgca ggcaataatt tccacttccg
    12061 ggcgcgtcag caaatgttcc acgacagtag taaacgcatc cttcgacagc tcgccattaa
    12121 aaacatcttt gccttcgctc actacccagc cgccgttttc agccacaaag gcaatttcat
    12181 tagcaatttc aggaaagaaa gagatcaact gataatattg attcccgctg gcgaccacaa
    12241 agcgaatgcc ttgtgctttc atttgctgat actgagccat aaaccgctca cggttatagg
    12301 ttttttgatc gcttaagaaa gtaccatcca tgtctaccgc aattaattta atgctcatca
    12361 actattctcc atcgccgtct gcgttttggt atcgggtttg gcgacggctt tcgcgacaat
    12421 agccgcgaga ataaccagcg ccagtacaac catcattgca ctacgtaatc cataatgttc
    12481 gccgagatag cccagcagcg gcggcccgac gaggaaagcc agataaccgg tcgtagctac
    12541 cacgctgacg cgggttggtg catcggggcc ggtatcgctg gcggcagaaa tggtcagcgg
    12601 gaagcccagc gaggcaccca gtccccaaag tacaacagac accccagcga cccaggcgct
    12661 atcgacaaaa ataatcagcc caatacccaa cgcccccatt agtgcactgg cccgaaccac
    12721 ggcaacgcga ctgtaacggt caatgaacca accgccggta aagcgtccaa cggtcatccc
    12781 cagagtaaaa ccggcataaa tcagcgagcc ggaagtaggg ctaaaaccgt gaccatcaac
    12841 cattaataag ggtaaccagt cgttggcaga accttcggca aaggccatcg ccagcactac
    12901 aacaccaatc aacagcaact ggatatcgcg ataaaaagga acgccttttt cgccatgctg
    12961 ggtgccatcg gcagcatttt tgcccgtacc atcagggatt gcctgaatgg cgatataaat
    13021 aggcgcgatg cctaccagcg ccgccaataa aatgtgcatc gttgccggaa cgccaaaggc
    13081 cgtcagtgcc atcccgacac cagcgcctgc cagcgtgccc aggctataaa aaccgtgcat
    13141 catcggtaaa accgtcttat tcatttctct ctcaacggcg gcaccttcaa cgtttatcgc
    13201 cacttccgca gaaccaaaac tagccccaaa gacgccgaga ccaacggcaa agagcaaggg
    13261 cgatgtcagc cagagtgcca gacttaatac catcatcccg atcaatgcgc aggacatcgt
    13321 gacaaggatg acattacgcg tcccaaagcg tttcactaac cacgccgagc agagaatacc
    13381 gctcatcgaa ccgatcgaca gaccaaagag aacaccgccc atttcagcga tcgagacaga
    13441 gagaatatcg cggatagcag gcgtacgggt tgcccaggac gccattaaca ggcctggcaa
    13501 aaagaagaac ataaacagcg cccaggttcg gcgtttcaat gcattacgtg aagaatttac
    13561 ggtcatagac cacgtcaaaa taagaagagg gaagacaaca ttagcaagac tgtgtacatt
    13621 tgtacacaat tgcagagaga ggaaatgaca tgcgtcgcgc taacgatccg caacgacgag
    13681 aaaaaattat ccaggccacg ctggaggcgg tgaaacttta cggaatacat gctgttacac
    13741 accgcaaaat tgctaccctt gccggggtgc cgttgggatc gatgacctac tatttttcag
    13801 gaattgatga gttgttgctg gaggcgttca gccgttttac tgagatcatg tcccggcaat
    13861 atcaggcatt ttttagcgat gttagtgatg ctccaggcgc atgccaggct atcaccgata
    13921 tgatctacag ctcacaggtc gcaacgccgg ataatatgga gctgatgtac cagctctacg
    13981 cgctggctag ccgaaaaccg ctcttaaaaa cggtaatgca aaactggatg cagcgcagtc
    14041 aacaaacgct cgaacaatgg tttgaacccg gaaccgcccg cgcgcttgat gcgtttattg
    14101 agggaatgac gctgcatttt gtcaccgacc gtaagccgct atcacgcgag gagattttga
    14161 ggatggttga gaggattgct gggtagtaga taaatttcag gcaacaaaaa acccatcaac
    14221 cttgaaccga aatggcgggg ttgatgggct ccacaaaatg gggacatcaa agaaaagcag
    14281 tggcactaat taagactgat gccctgcgga aaagttctgc ggttgtgcaa aaaaaatttc
    14341 attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga tgattgtccc
    14401 tgccagcgtc agcaggacgt tggcgattgc ataggtgccc gcatagccca gcgccgggat
    14461 gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc gtgcgcccat
    14521 cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga acaagaaaca
    14581 gataaccact ggcacaagac tgacaatcaa tccggcaatc aacatctgac cgccaatcgc
    14641 gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca taaacaccat
    14701 taagccgaac tctttcacca tgcttaatgc accctgcgga atgtaaccga aggtcgggtg
    14761 gttagcgcgc ataaatccca gcataattcc ggcgaataac aacccggcag cgtttcccat
    14821 gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc caataacaaa
    14881 gaaggcgcag aatgccagca agtcagtgac ctggctatga atcgagataa agccgatgcg
    14941 atcggcgatg gtttttacgc gacgggcatc gccgctgact tgtaaaacgt cacctttgtt
    15001 aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc ggttaaggaa
    15061 gcaaccgtga tcggtcaact tcagttgtgc gagacgtttg ccaacggcgt tatggttttt
    15121 aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga aaacttcttt
    15181 gccgttacgg aagctgggat cgagtcgggc gtgggcgtcg ggatagccta ccaacgctat
    15241 ttcatcgccc atttgtagca ccgcatcacc gtctggattt gccagaatcc cgttacgtcg
    15301 aatacgttca atgtagcagc cagtttgtcg ataaataccc agttcacgca gatttttgcc
    15361 gtcggtccag gccaccagct ccgggccgac gcggtaggcg cggatcaccg gtaaataaac
    15421 cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg cgctggtctg
    15481 taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac tcaccagacc
    15541 gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg agagctgcct
    15601 gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg gtgtcgacgt
    15661 catagagcca gctaacatac cggccgtcag gccaatatcc cagccaaaca gcttacctaa
    15721 ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta ggtaattttt
    15781 cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga cgcagaaaat
    15841 aaacagcata aagccaagat taagcgcgtc ggtgttaatg ctgaaatgtt gttggcctaa
    15901 taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac caagtcgtaa
    15961 tttcccgaga catagcccaa gcgcgaggac cacaaataat aacagaatgt aattcccatt
    16021 taacaattcg gcgacgttta tattcacgga ggctaacttc ttgtttacta gtaagctgtt
    16081 gaaagaaatg gtaatttacg ataatgtttt taccagaatt cagggcgcag attcattcag
    16141 cgcacctaaa cgatagtaaa gtaacaatat attttactag tgtaatcaca ttaggtatca
    16201 acggctatat gaattgcgtt ggcctatatt agcatggaat gcgaagcggc tttatcttac
    16261 tgaacgccac actggcgaaa aatgtgttcg atagacgcag tgtcaggagg aacgagtgaa
    16321 acataaacaa cgttgggcgg gggcaatctg ctgttttgtc ctcttcattg tggtgtgtct
    16381 ttttctggcg acgcacatga aaggcgcttt tcgggctgcc gggcatcctg aaatcggttt
    16441 gctatttttc attcttcctg gtgcagttgc cagcttcttt tcacatcgta gagaagtcct
    16501 gaaacctctg tttggcgcaa tgctggcggc accctgctcg atgctcatta tgcggctgtt
    16561 tttttcaccg acgcgctcat tctggcaaga gctggcatgg ttattaagtg cggtgttctg
    16621 gtgtgcgctg ggggcattgt gtttcttatt tatcagtagt ttgtttaaac cacagcacag
    16681 aaaaaatcaa taaagccctc aatgcgaggg cttgtcagac gatcaggcgt ccagattctc
    16741 tttcacccat gcagcaaaat cggtatagcc gccgatatgt tgctgatcga caaaaatctg
    16801 cggcacggtt tctacgggtt tacctgcctt ttgttgtaga tcttctttag tgatcccttc
    16861 cgcacgaata tctacatact gatactggaa atcatcgcgt tcattgctca atttctcagc
    16921 cagatctttt gcacgcacac agtaagggca acccggacga ccaaaaataa cggtttgcat
    16981 tatttctctc ctcatagatt tatgcctgta atgatcacgc taaaatgtat tcgctgaaag
    17041 taggtttaac ctgttgcatt aattgctaaa agctataact gttaaacaca atacagtgaa
    17101 aagttttaga ctgaaggctc actttgcaga gggaagcgta tgcgcgcgat cggtaaattg
    17161 cctaaaagcg tgttgatact ggaatttatc ggaatgatgt tgctggcggt ggcgctgctg
    17221 tcggtaagcg actccctgtc gctacctgag ccattttctc ggccagaagt gcagattctg
    17281 atgatttttc tcggtgtttt gctcatgctt cccgctgcgg tggtggttat tcttcaggtg
    17341 gcaaaacgtc ttgccccaca gctgatgaac catccaccgc aatattcacg ttcagaaaga
    17401 gaaaaagata atgacgccaa ccattgaact tatttgtggc catcgctcca ttcgccattt
    17461 cactgatgaa cccatttccg aagcgcagcg tgaggcgatt attaacagcg cccgtgcgac
    17521 gtccagttcc agttttttgc agtgcagtag cattatccgc attaccgaca aagcgttacg
    17581 tgaagaactg gtgacgctga ccggcgggca aaaacacgta gcgcaagcgg cagagttttg
    17641 ggtgttctgt gccgacttta accgccattt acagatctgt ccggatgctc agctcggcct
    17701 ggcggaacaa ctgttgctcg gtgtcgttga tacggcaatg atggcgcaga atgcattaac
    17761 cgcagcggaa tcgctgggat tgggcggggt atatatcggc ggcctgcgca ataatattga
    17821 agcggtgacg gaactgctga aattaccgca gcatgttctg ccgctgtttg ggctgtgcct
    17881 tggctggcct gcggataatc cggatcttaa gccgcgttta ccggcctcca ttttggtgca
    17941 tgaaaacagc tatcaaccgc tggataaaga cgcactggcg cagtatgacg agcaactggc
    18001 ggaatattac ctcacccgtg gcagcaataa tcgccgggat acctggagcg atcatatccg
    18061 ccgaacaatc attaaagaaa gccgcccatt tattctggat tatttgcaca aacagggttg
    18121 ggcgacgcgc taaaactgcc acgtcgatgt atgatacgcg ggcttttgac caggtctgac
    18181 agagaggtgc agggtgaaaa ttgccatatt gtcccgggat ggaacgctct attcgtgtaa
    18241 gcggctgcgt gaagccgcta tacagcgcgg tcacctggtt gaaattcttg atccgctttc
    18301 ttgctatatg aacataaatc ctgcggcgtc ttctattcac tacaaaggcc gcaagttacc
    18361 ccattttgac gcagtgatcc cgcgtattgg caccgccatt accttttatg ggacggcggc
    18421 actgcgccag ttcgagatgc tggggagcta tccgctcaat gagtcggtcg ccattgcccg
    18481 ggcgcgtgac aaattgcgtt ccatgcaact gctggcgcgt cagggcatcg acctgcctgt
    18541 cacgggcatt gcgcattcgc cggatgatac cagcgattta atcgacatgg tcggtggtgc
    18601 gccgctggtg gtcaagctgg ttgaaggcac gcagggaatt ggcgtcgtgc tggcggagac
    18661 gcgtcaggcg gcggaaagcg tgattgacgc tttccgcggt ctgaacgcgc atattctggt
    18721 gcaggaatat atcaaagagg cgcaagggtg cgatatccgc tgtctggttg ttggcgatga
    18781 agtagtcgct gcgattgaac ggcgggcgaa agagggcgat tttcgttcca atttgcatcg
    18841 tggcggcgcg gcaagcgtcg ccagtatcac accacaggag cgtgaaatcg cgataaaagc
    18901 cgcgcgaacg atggcgctgg acgttgctgg tgtggatatt ctgcgtgcta atcgcgggcc
    18961 gttggtgatg gaagtgaatg cgtcgccagg gctggaagga atagaaaaaa ccaccggtat
    19021 cgacatcgcg ggtaaaatga tccgctggat cgaacgccac gctacgacag aatattgcct
    19081 gaaaacgggt ggttagtcgc aatcacatta ctgatcatgg ttttgcctgc gctttttgcg
    19141 taagctgtgc cggtcttttt atcggaagag gttgtacaaa attatgacat cgctggtcgt
    19201 tcctggtctg gatacgttgc gtcaatggct cgatgacctg gggatgagtt tttttgaatg
    19261 tgataactgt caggctctgc atctgcccca tatgcagaat ttcgacggtg tctttgatgc
    19321 taaaatcgat ctgatcgata acacgatcct gttttctgcc atggcggaag tccgaccttc
    19381 agccgtattg ccgctggcgg cggatttatc agccatcaat gccagttcgc tgaccgtgaa
    19441 agcatttctt gatatgcagg atgataatct gccaaagctg gtggtttgcc agtctttatc
    19501 cgttatgcag ggcgtaacct atgagcagtt tgcatggttc gtgcgtcaga gcgaagagca
    19561 gatttcgatg gtcattcttg aagctaatgc ccatcaactg ctgttaccga ctgacgatga
    19621 agggcaaaat aacgttaccg aaaactattt cctccactga taacttcttt cgagcacgca
    19681 gtcgctgccg cagtgactgc gtgccgcaaa attatccgct gattttaacc ttttattaaa
    19741 gattaattca cttctcttgt gccgatttgg ctttatcaca tagagcaaat acgcataaaa
    19801 atttgttaaa taccgttttt taatccgagc tatagtctca aaccctggct aaagttattc
    19861 ttgcgatgct tttatatagt gagcagtgca ggccgggaga gagttctctt ttcttacacc
    19921 gcgccgataa aaatatgcac gtttattgca tatctttcag tgtgacaact tttgttcgtt
    19981 tgttaacgaa ttttcagaag gaaagagata tgatcgcctt aaataaaaaa tggctatcgg
    20041 gtctggttgc gggtgctctg atggccgttt ctgtcagcac gctcgcggct gaacaaaaaa
    20101 cactccacat ttataactgg tctgattata tcgccccgga cacggtggcc aactttgaaa
    20161 aagaaaccgg tattaaagtc gtctatgatg ttttcgattc taacgaagta ctggaaggca
    20221 aattaatggc ggggagcacc ggctttgatc tggtggtgcc gtcagcaagc tttcttgagc
    20281 gccagctgac agcgggagtt ttccagccgc tggataaaag caaattgccg gagtggaaga
    20341 atctcgatcc ggagctgctg aagttggtcg ccaaacacga tcccgacaat aaatttgcta
    20401 tgccctatat gtgggcgacg accgggattg gctataacgt cgataaagtt aaagcggtgc
    20461 tgggcgaaaa cgcgcccgtc gatagctggg acttgatcct caaacctgaa aatctggaaa
    20521 aactgaaaag ctgcggtgtc tctttcctgg atgcgccaga agaagtgttt gctaccgtgc
    20581 tgaattatct cggcaaagat cctaacagca ctaaagcgga tgattacacc ggaccggcta
    20641 ccgatctgct gttaaagctg cgcccgaaca ttcgttattt ccattcatcc caatacatca
    20701 atgatctggc gaatggtgac atctgtgtcg ctattggctg ggcgggcgat gtctggcagg
    20761 cgtcaaaccg cgcgaaggaa gcgaagaatg gcgtgaatgt ctcgttctcg attccaaaag
    20821 aaggggcgat ggcgttcttt gatgtattcg ccatgcctgc ggatgccaaa aacaaagacg
    20881 aagcctatca gttcctgaat tacctgctgc gtcctgatgt ggtggcacat atctctgacc
    20941 atgtgttcta tgctaacgcc aataaagcag ccacgccgct ggtgagtgcg gaagtccgtg
    21001 ataacccggg tatttatccg cctgcggatg ttcgtgcgaa gctgttcact ctgaaagtgc
    21061 aggacccgaa aatcgaccgt gtgcgcaccc gcgcctggac caaagtgaag agcggaaaat
    21121 aatccgcagc cgtagatgcc ggaggggcgc accacacccg ccggcaattc gcaccatcat
    21181 ggtgcgcttg cacacattca atgccggaga gcagccgtga atgacgctat ccctcgcccg
    21241 caggcgaaaa cccgtaaggc gctgacgccg ctattagaaa tccgtaacct gaccaaatcc
    21301 tacgatggtc aacatgcggt ggatgatgtc agcctgacca tctacaaagg tgaaatcttc
    21361 gcgctgctgg gcgcatccgg ctgtggcaag tccacgctgc tgcgtatgct ggcaggtttt
    21421 gaacaacctt cggccgggca gattatgctc gatggcgtcg atttgtcaca ggtgccgcct
    21481 tacctgcgcc ccatcaatat gatgtttcag tcttacgcgc tgtttccgca tatgaccgtg
    21541 gaacagaaca tcgcttttgg cctgaaacag gacaaactac cgaaagcgga aattgccagc
    21601 cgggtcaatg agatgcttgg gctggtgcat atgcaggagt tcgccaaacg caaaccgcat
    21661 cagctttctg gtggtcagcg tcagcgtgtg gccctggcca gaagccttgc caagcgcccg
    21721 aaactattac tgctcgatga gccgatgggc gcgctggata aaaagctgcg tgaccggatg
    21781 cagcttgaag tggtggatat tctggagcgc gtcggtgtga cctgcgtaat ggttactcac
    21841 gatcaggaag aggcgatgac tatggcgggg cgcatcgcca ttatgaatcg tgggaaattt
    21901 gtccagattg gcgagccgga agagatctac gagcatccga ccactcgcta cagtgctgaa
    21961 tttatcggtt cggttaatgt ctttgaaggt gtactcaaag agcgtcagga agatggtctg
    22021 gtgctttatt cgccggggct ggtgcatcca ctgaaagtcg atgcggatgc ttcagtggtc
    22081 gataacgtgc cggtgcatgt ggcgcttcgc ccggaaaaaa tcatgctttg cgaggaaccg
    22141 cccgccaatg gttgtaactt cgcggtgggg gaggtgatac acattgccta tctcggcgat
    22201 ctttcggtgt atcacgttcg tctgaaaagt gggcagatga ttagcgccca gctacaaaac
    22261 gcccatcgtc atcgtaaagg gttaccgacc tggggcgacg aagtgcgttt gtgctgggaa
    22321 gtggacagct gtgtggtgct gacggtttaa ggagcaaaga tgagtacact tgaacctgct
    22381 gcccagccaa aaccctttaa gctgtggttg tcgcagctgc aaatgaaaca tgggcgcaaa
    22441 ctggttattg cgttgccgta tatctggttg atcttgctgt ttctgctgcc atttctgatc
    22501 gtctttaaaa taagcctggc ggagatggcg cgcgctattc caccttatac cgaattgatg
    22561 gagtgggctg acgggcaact ttctatcact cttaatctcg gtaattttct gcaactgacc
    22621 gacgatccgc tctattttga tgcttatctc cagtcgttac aggtggcggc gatttcgact
    22681 atttgctgtt tactgatcgg ctatccgctg gcgtgggcgg tggcgcacag taagccttcg
    22741 acccgtaata tcttattact actggtgatc ctgccgtcgt ggacctcgtt tctgattcgt
    22801 gtctacgcct ggatgggaat attaaaaaac aacggtgtgc tgaataattt tctgctgtgg
    22861 ctggggatta tcgatcaacc gctgaccatt ctgcatacca actttgcggt ttatattggc
    22921 attgtttacg cctacgtgcc gtttatggtg ctgccgattt acaccgcgtt gattcgtatt
    22981 gattattcgc tggtagaagc ggcactggat ctcggtgcac gaccgctgaa aacgttcttt
    23041 actgtgatcg tgccgctgac taaaggtggg attattgccg gatcgatgct ggtgtttatc
    23101 ccggctgtgg gcgagtttgt gatcccggaa ctgctcggtg gcccggacag catcatgatc
    23161 gggcgcgtgc tgtggcagga gttctttaac aaccgcgact ggccggtggc ctcagcggta
    23221 gcgatcgtca tgttgctgct gctaattatg ccgataatgt ggttccacaa acatcagcaa
    23281 aaaagcgagg gagaacacgg atgaataatt taccggtggt tcgttcgccc tggcggattg
    23341 tgattttgct gttgggcttc acctttctct acgcgccaat gctgatgctt gttatctatt
    23401 cgtttaacag ctcgaagcta gtgacggtgt gggccggctg gtcaacgcgc tggtatggcg
    23461 aattattgcg cgatgacgcg atgatgagtg cggttgcttt aagtctgaca attgcggcct
    23521 gtgcggcgac ggcggcggcg atcctcggga ctattgcggc ggtcgtgctg gtgcgctttg
    23581 gcaggtttcg cggatcaaat ggttttgcct tcatgatcac cgcaccgctg gtaatgccag
    23641 atgtcatcac gggtttgtcg ctgttgttgt tattcgtcgc gcttgctcat gctattggct
    23701 ggcctgcgga ccgcggtatg ctcaccatct ggctggcgca tgtcactttc tgtacggctt
    23761 atgtggcagt cgttatttcg tcgcgtctgc gggaactgga tcgctcgata gaagaagccg
    23821 cgatggatct cggtgcgacg ccgctgaaag tgttttttgt cattacgcta ccgatgatca
    23881 tgcccgcgat catttctggc tggttactgg cttttacttt gtcgcttgat gatctggtga
    23941 tcgccagctt tgtttccggg ccgggagcca ccacattacc gatgctggtc ttttccagcg
    24001 tgcggatggg ggtgaatccg gaaatcaacg ccctggcaac attgatcctc ggcgcggtcg
    24061 gaattgtcgg atttatcgcc tggtatctga tggcgcgcgc agaaaaacag cggatacgcg
    24121 acatccagcg tgcaagacgt ggctgaagac actaaaattt gccaacctgg ctacataatg
    24181 ccgcgcatgt cgcggcattg ttttcatgga agacgaaacg ttgggatttt ttaagaaaac
    24241 atcttcatct catgctcgcc tgaatgtgcc tgcgctggtg caggtggcgg cgctcgccat
    24301 tattatgatc cgtggcctcg acgtgctgat gattttcaat acgctgggcg tgcgcggtat
    24361 tggcgagttc attcatcgca gcgtacaaac ctggagttta acgctggtct ttttgagcag
    24421 tctggtgctg gttttcattg agatctggtg tgcgttttca ctggtgaaag ggcgtcgctg
    24481 ggcgcgctgg ctgtatctgt tgacacaaat cacagccgca agttacttgt gggcggcttc
    24541 gctggggtat ggttatccgg agctgttcag cattcccggt gaatcaaaac gtgaaatctt
    24601 ccatagcctg atgctgcaga agctgccgga tatgctcatc ctgatgctgc tgttcgttcc
    24661 ctcgaccagt cggcggttct tccagttgca ataatgtgta taatcgtcgc ccctgatgat
    24721 gtgaaggtca atgtatgcag tgcgcacttt acgacgcggg tcgctgtcgt tcctgtcagt
    24781 ggataacgca gccgattcca gagcaactct ccgctaaaac tgccgatctt aaaaatctgc
    24841 tcgccgattt tccggttgag gaatggtgtg cgccggtgtc aggcccggaa caagggtttc
    24901 gtaataaagc caaaatggtg gtgagtggta gcgttgaaaa accactgctc ggtatgctgc
    24961 atcgagatgg tacaccggaa gacctttgtg actgcccgct ttatccagcc tcatttgcgc
    25021 ccgtttttgc ggcgctaaaa ccgtttatcg cccgtgcggg gttaacgccc tacaacgtgg
    25081 cgcgtaaacg tggtgaactg aaatacattc tgctgactga aagccagagc gatggtggca
    25141 tgatgctgcg ttttgtactg cgttctgata ccaaactggc gcaattgcgt aaggcgctgc
    25201 cgtggttaca ggaacaatta ccgcagctga aagttattac cgtcaatatt cagccggtac
    25261 atatggcgat tatggaaggg gagacggaga tctacctgac cgaacaacag gcactggcag
    25321 agcgttttaa tgacgtaccg ctgtggatcc gtccgcaaag tttcttccag actaatccgg
    25381 cggtcgccag ccagctgtac gccaccgcgc gcgactgggt acgacagctg ccggttaaac
    25441 atatgtggga tctgttctgc ggtgtgggtg gctttggttt acactgcgcg acgcctgaca
    25501 tgcagttaac cgggatcgaa attgcaccag aggccattgc ctgtgcgaag cagtcagccg
    25561 ctgaactggg cttaacgcgt ttgcaatttc aggcgctgga ctctacgcag tttgccaccg
    25621 ctcaggggga agtgccggag ctggtgctgg ttaacccgcc gcgccgcggc atcggtaaac
    25681 cgctgtgtga ttatctctca acgatggcac cgcgttttat tatctactcc agctgtaacg
    25741 cccaaactat ggcgaaagat atccgcgaac tgcctggtta tcgcatcgaa cgggtacagc
    25801 tttttgatat gttcccgcac accgcgcact atgaagtgct gacgctactg gtgaagcaat
    25861 aaaaaagccg caggtgcggc tttctgaatc ttactgtggg aaccactggt cattgatttt
    25921 ctgatatgta ccgtcagctt taattgctgc cagcgcgtta ttcagttttt ccagcagagc
    25981 tttgttatcc ggacgtacag cgatacccag gccggtgccg aagtattgcg gatcggtcac
    26041 tttttcagta gcaacgccca gttgcggatt ggttttcagc cattcgttta ccactgcggt
    26101 gtcaccaaat acgccatcaa tacggccatt tttcagatcg ataaaggcat tctgataact
    26161 gtcataggag acagttttca cttccgggtg ctgatcctga atatatttct ggtgcgtggt
    26221 gccgttttcc atcccaatac gtttgccttt cagatcggca aacgttttgt aggtatcttt
    26281 tttggcaatc acgacggctg agttttcgta gtagggcgtg gtaaacgata cctgtttgct
    26341 acgctccggc gtgatatcca ttccggagat tacggcgtca tattttctga atttcagtga
    26401 cgggatcagg ctgtcgaaag cgtgattagt aaaagtacat tctgcctgca tttgtttgca
    26461 caaggctttt gccaggtcga tatcaaagcc gacaatctca ttattagcac ctatagattc
    26521 aaagggtgga taggtggctg aaacgccaaa attgattttc tctgcggcag aagcaccgaa
    26581 agtaaaagaa gcaagtaaag cggcaagaac taactttttc atgatggaac tcccgtctgt
    26641 caatcttatg atttttggcc gtgtctgcgg catgggataa caatgccatt aagtgaattt
    26701 atatgcaata aatatgagta aataatttat atgaaataaa aaaagacgga cagcttagtg
    26761 ggttgtccgt cttcataata agaatttatg cactatgtag gccggataag gcgtccacgc
    26821 cgcatccggc atcagcatgc agctacatta attccgccgt tcgaatgcca gtgctttacg
    26881 ctcgatcaga cgcatcatca gcgtcagcag gccgttgacg accaggtaaa taatccccgc
    26941 cgcgccaaat accattacat cgtaggtgcg tccgtacaac aactggctgt atcccatcac
    27001 ttccatgagc gtaatggtgt aagccagaga ggtacttttg aacaccagca ccacttcgtt
    27061 ggaataagaa gagagcgagc gtttaaacgc gtacggcagc aggatcgcca gcgtatcttt
    27121 tttgctcatt cccagggcgc tacaggactg ccattgacct tccgggatcg cgcgaatcgc
    27181 accgtaaaac agctgcgtgg tgtacgccgc actgttcagc gacaacgcaa tcagcgcaca
    27241 taaccacggt tctgacaaca aatgccacag tgccggatac tcctgcaacg tcgggaactg
    27301 gcccggcccg taataaatca ggaagatctg caccagcagc ggcgtaccgg taaacagcgt
    27361 gatatagccc cgtaccagcc acaccagcac cggcgttttc agcgtcagga tgatggtaaa
    27421 aatcaatgcc agaatcagtg ccacaatcag cgaggcaacg gttagcgtca ggctggtgtg
    27481 cagccctttc atcagttcgg gtaaatactc aaacattagc tgggcctccg ctcaaaacgt
    27541 gtcgcgcgca ggtcaatgcg tttgagaatg tactgactga gcagggtgat caccaggtaa
    27601 atcgccgccg ccacaatgta ccaggtaaac ggttcctggg tacgggtagc gatgcttttg
    27661 gtttgtagca ttaaatcatt cacactaatc aaactgacca gcgcggtatc tttcagcagc
    27721 accagccact ggttaccgag acccggcagc gcatgacgcc acatctgcgg catcaccaga
    27781 cggaaaaaga tagccgattt cgacagcccc agcgcctgac cggattccca ctgccccacc
    27841 ggcaccgctt ttaatgcacc acgcagcgtt tgcgaggcat aggcggcata cagcagtgac
    27901 agagcgatga caccacaaag gaacgggcta acgtcgaagt tctcaatgtc catctgcacc
    27961 gggatctgca cgaacccaag attgatagtg aagccatccg aaagcgtcag cagcagctgc
    28021 gaggagccaa aatagataaa cagcaccacc agaatttctg gcaggccacg cagaatggtt
    28081 accagcgctg aacctgccca cgcgacagga cgccatttcg cagactccca taccgcaaag
    28141 aacatcgcca gcgccagccc gacaatcaat gcacaaacgg caaggccgac ggtcatcccg
    28201 gcggcgcttg ctaaaggaaa aaattcattc atcaggaatt acttctggaa ccatttgttg
    28261 tagatggttt cgtaagtgcc atctttcttc actttttcca gcgcagtgtt gaatttctgc
    28321 tgcagttcag tgttgccctg gcgtactgcg atgccgaggc cagtaccgaa gtaatcttta
    28381 tcggtcactt tatcgcccac tgccgccagt ttcgggttat ctttcagcca ttcagttacc
    28441 actgcggtgt caccaaatac gctgtcgata cgaccgtttt gcagatccag ttttgcgttc
    28501 tggtaactgt catatggcac ggtagtgatt tccggatgct tatccataat gaacttctgg
    28561 tgtgtcgtcc cgttctgaac gccaactttt ttgcctttca gctgatcaac actggtgtat
    28621 ttgccttgct gacccacaaa cagggcagag ttgtcatagt acggggtggt aaacagcacc
    28681 tgcttttcac gctccggagt gatatccatg cccgccatca ctgcttctac gcgacggaat
    28741 ttcaggcttg ggatcaggct gtcaaacgcc tggttagaga aagtgcaggt tgcatcaatc
    28801 tctttacaca atgcctgcgc caggtcgaca tcaaaaccaa cgatctggtt gtttgcatca
    28861 atcgattcaa acggaggata ggaggcttcg gtggcaaaac gaatggtttc ggctgctgtg
    28921 gcggaaagac taaaacctgc aattaacgcg gcaatcagaa cttttttcat tgttgttatc
    28981 ccgaatctta gtgagagaga tagtttttaa atgcttcggt ttgcggctcg gtaaagcagc
    29041 tcgcgtcgcc ttgttctacg atatgaccgt tttccatata caccactcgg ctggcggttt
    29101 tacgcgccac ttcaacttcg tgggtgacga tcacctgggt aatattcgtt tctgccagct
    29161 cacgaatgat gctgacgatt tgtgccgtaa tttcggggtc cagtgcggcg gtcggttcat
    29221 cgaacagcag tacctgcggt tccatcatca acgcacgggc aatagcaaca cgctgctgct
    29281 gaccaccaga aagatgcagc gggtaacgat cgctataagg tttaagacgc agacgttcca
    29341 gcagtttttc tgcacgggcc agcgcctgat ctttactcag ccccagtaca cggcagggcg
    29401 cttcaatcag gttttgctgc acggtcagat gcggccacag gttgtattgc tgaaacacca
    29461 tgccaacgtt acgacgcaaa tcgcgaatcg ctttgtcaga gggggttttg gtgaaatcga
    29521 aatggttgcc tgcaatgttg agcgtaccgg agcgcggcat ctcaagcaga ttgagtacac
    29581 gcagcagcga gcttttaccc gcgccgctgg ggccaagtaa caccagcgtt tcgccctgcg
    29641 ggcaatccag cgtgatatcg aacagcgcct gatgcgcgcc gtagaagcaa ttaatgccgt
    29701 ttaattgaat actcattgac actcgtatac tggcagtctg atagctattg aggtcgaaga
    29761 tagtaccttt gacagaataa ttatgcaata tttctgcttt aaaagttaaa agcaaagcgc
    29821 attattcaat aaacatagca caaaataacg ggggcggtgg tcggcgagca taaatgtcgg
    29881 cattcctcac gaaatgccgg acaatttacg gggtttattg gttgatcaag acgttagcga
    29941 ttctcgatgg attgacggag cgtacccgcc gtggcatgaa cgctaccgcc taagtagcgc
    30001 acatcgtcga tgacccaaca ctggccttcc tgaatcatta acacttcatc ctgccagccc
    30061 tggtcaccct gcttgagatc cacgcgcaat ggaatgttac gggcatcacg attagggata
    30121 gtcgatgcac tggctacgtg ggcgctgtct ggcaaggtgg ttcgactgga gaacggatcg
    30181 ctgcttaaca attcacgatg actattatcg cggctggcat cgctaagtag ggttgccagt
    30241 ttgtcactca aataggggcg cagagcggta atgtcattgc tgcgatgttg aatgcggtag
    30301 tcatagaact gttgagccac actatcaggc cccccctgga cacaggaacc ggtgcggggg
    30361 ccgttatctt tataagctgg cgtgacggtg gtacaggcac tgagtagcag tgcgcaaggg
    30421 ataagcatgg tcaatttgct atagcgcatg atgatttcct tataagcgaa caccttgaaa
    30481 gcgttctacg ataataatga tatcctttca ataatagcgt atcagtccga taatgctttg
    30541 aagatcgaag gcttagcaaa caaggagatc gatcatgcaa ttttctacaa ccccaactct
    30601 ggaaggccag accatcgttg aatattgcgg tgtggtgact ggcgaagcga ttttaggcgc
    30661 gaatattttc cgtgatttct ttgccggtat ccgcgatatc gttggcggac gctccggtgc
    30721 ctacgaaaaa gaactgcgta aagcgcggga gattgccttt gaggagctcg gctcccaggc
    30781 gcgagcgctg ggggctgatg ccgtcgtcgg tattgatatc gactacgaaa cggtcgggca
    30841 aaacggcagt atgctgatgg ttagcgtcag cggtacggcg gtgaaaacgc gtcgatgaga
    30901 agagtcttct ggctggtcgc tgccgctctg ttactggcag ggtgcacagg cgaaaaaggc
    30961 attgtcgaga aagagggata tcagcttgat acccgacgcc aggcgcaggc ggcgtatccg
    31021 cgcattaaag tgctggtgat ccactacacc gcagatgatt ttgatagctc gctggcgaca
    31081 ctgaccgata agcaggtcag ctcgcattat ctggtccctg cggtgccacc gcgatacaac
    31141 ggtaaaccgc gcatctggca actggtgccg gaacaagaac tggcctggca tgcggggatt
    31201 agcgcctggc gcggggcaac gcgtcttaac gacacctcta ttggcattga gctggaaaac
    31261 cgtggctggc aaaaatcggc gggagtgaaa tattttgccc cgtttgaacc ggcacagatt
    31321 caggcgctta ttccgctggc gaaagatatt attgcccgtt atcacatcaa gccggaaaac
    31381 gtagtagcac atgcggatat tgcgccacag cgcaaagacg atccggggcc attatttccc
    31441 tggcagcaac tggcacagca ggggattggt gcctggccgg atgcgcagcg ggtgaacttt
    31501 taccttgccg ggcgcgcgcc gcacacaccg gtagagaccg cgtcattgct ggagcttttg
    31561 gcgcgttatg gttatgacgt aaagcctgat atgactccgc gcgagcagcg gcgtgtgatt
    31621 atggcgttcc agatgcattt ccgcccgacg ttatataacg gcgaagcgga tgcagaaact
    31681 caggcgattg ccgaagcgtt gctggagaaa tacgggcagg attagcgcgg cagttttccg
    31741 tggtcgcgca gccaggcggc agttttctcg ataccttcat ccagggtgag gactggctga
    31801 taacctaact cttcctgcgc acgcgtaata tccagcgtaa agtcaaaatt caacttggag
    31861 acgccgtagt gggtcagcgg cggctctttt gctgacttgc ggcctaaacg ctccatgctg
    31921 cgggcgatca tatccagcat cgggtaagga accgaacgaa tacgacagtc aatattcaac
    31981 tcgtcgatca gcttctgcac gatgctgcgc agtgtgcgat gctcgccgtt ggtgatgttg
    32041 tacacacgcc cggaaggtag cttatcgcac gcttcctggc ttgccagcca catggcgtgc
    32101 acggcatttt catagtaggt catatcgacc agcgcactgc cgccatgcgg taacagaata
    32161 ctgccgtagt gatgcatcat atgcgccaga cgagggataa agactttatc gtgcggtccg
    32221 aacagacttt gtgggcgcag aatagtaaag cgcgtttgtg gattcgcctg cgaaagcata
    32281 ttgatcactt cttcgctggc tgctttgctg cgggcaaact cgttggcgaa gcggtgaggg
    32341 cgaaaatctt ctttaatatc gcgatggtgg tgataatcga agtacaggga gggagaagag
    32401 atatgaataa agttacgcac accccaggcg acagcccatt cacccaggcg gcgagtggcg
    32461 cgaacgttag ccagatcgaa agcctgttgt gtcccccagg gcgaggtaaa gctggagcag
    32521 tgccacagcg tatcaatgcc cgcgagcatc actttagctt gtgatgagac caactcggtc
    32581 agatccgccg gaacaaactc tgcgcccatt ttttccagca atttacccat tgcctcgttg
    32641 cgaccggtcg ctcgcacgct gatgcctttc tggcataaaa actctaccgc gtttcgacct
    32701 aagccgctgg tggcgccggt aaccagtacc ttcatatcaa tccactgttg ttgagaaaat
    32761 gacgtgcgca ttcttccgtg atttccccct tgatgcaatg ggaaacatga aagaataacg
    32821 caggttttgt cgattaatct gtgctttgtt ctgccagtct ggcgattcgt tttgccattc
    32881 cgcgaaaaat aaacagatgc gcggggatca tcaataacca gtaaaacagc cccggcatac
    32941 cgtgcggatg ccagaaagca cggacatcga ttgtacgata gtcgccttta tcttccaggg
    33001 taaaacagag tcgtcccagc cccggcgctt tcatgccaaa taacaacgta agttgttttt
    33061 ccggttcaac gacaatgact ttccagctat ccacagcatc gccggtctgt aaatattcgc
    33121 gctccgggcg gccttttgcc agcttatgac cgatcgcaag gtccatcaac gcccgtgtct
    33181 gccacaaaat attgccaaag aaataacgct ctttaccgcc gatttgattg accacccgcc
    33241 atagcgcctg aaggctggca gacgttttaa cggtaaaccc cgcctgtttg gcaaaataac
    33301 cgtattctgg tcgccagcga gcaaaagcct gcgcgtcata tccccagtcg ctggagttga
    33361 ccagtttttc ttcctctttc aacgtgctac gtaccgcgtc atcgaaagcg atcagcggtt
    33421 gtggaatgag tgcacgtaac gcggtgtcat ccgccagcag atcgtgtttc agcccctgaa
    33481 tcaacgctct ggcggtggtg ggcggtactg aagtaatcac attaagaaac cacaccgaaa
    33541 tccagcgggt ggggaggggg atggggagca accagcggcg cttaccgctc accgccataa
    33601 aatgttcaaa ctgttgctga taactgagca cttctggccc agcggcttcg aagatgcgat
    33661 gttcgcaagc cggatgatct aacagcgcca caagatagtg cagcaggttt tccagcgcga
    33721 tgggcgtagt gcgtgaacgt acccagcgtg gtggcgttaa tactggcagg ttgtaaacca
    33781 tatcgcgcat gacttcgaat gccgctgaac ccgcgccaac gataattccg gcacgtagtt
    33841 cggtcaccgg gacacccgct tcacgtaaaa tatccgccgt ggcctgacga gcacgcagat
    33901 gatcagattg ttcatgaggc ggtgcctgca atgaactgag aaagatgact tgctttaccg
    33961 gtacttcgcg cagcgcatcg cggacgttga gagccacctg gcgctcctga gcgataaaat
    34021 caccaccttc gcccatgctg tgcaccagaa aatagacagt atcaatattc tgcaacagga
    34081 ccggaaggtt atccggccag ttgagatcga ctttatggca actgacatta gccagttgca
    34141 gttttgcaag tctgtcgacg tgacgtgccg ccgccaggat ctgatgccct tgctggctga
    34201 gtgtgtgcac cagatgttga ccaatgtagc cactggcacc gagaactaaa atgcgttgcg
    34261 gcacgtctct ctccttaacg cgccaggaac gcacgccagt gggcggcaac ttccgccagt
    34321 tgttcacgcg agacgtcaag atgggtcacc aggcggacaa tcggcgaggc gttaatcagc
    34381 acgtttctcg ctttcatgta ttcgcctaac gcggcagcat tttcttcccc gacgcgaaca
    34441 aacagcatat tggtgtcctg acgcatcaca tccgcgcctg cttcacgcaa ctgctccgcc
    34501 atccaggcgg cgttatcgtg gtcttcctgc aaacgtgcga cgttattttt cagcgcatac
    34561 atcccggcgg cagccagaat gccggactga cgcatcccgc cacctgtcat tttccgccag
    34621 cgaatggcac gtttaatgta atcacgatta ccgacgagta atgaaccgac tggcgtccca
    34681 agacctttcg acaggcaaat ggtgaacgag tcacaatatt gcgtgatctc tttcagttcg
    34741 cagccgtaag ccaccacggc attaaaaatg cgcgcaccgt caacgtgcag cgccagattg
    34801 cgctcgcggg taaattccca tgcttctttc aggtattcac gcggcagcac tttgccgttg
    34861 tgggtgtttt ccagactgag taatttggtg cgggcgaaat ggatatcgtc gggtttgatt
    34921 ttcatcgcca ctttatccag cggtagcgta ccgtcggcag ctgcgtcgat gggttgcggc
    34981 tgaatactgc ccagtactgc tgcgccaccg gcttcaaaca gatagttatg cgcggcctga
    35041 ccgacgatat actcttcgcc gcgttcgcag tgactgagca gagcgaccag gttggcctga
    35101 gtgccggtag gcagaaaaat ggcggcttct ttaccggaaa gctctgctgc atagtcctgc
    35161 agagcattaa cggtagggtc gtctccataa acgtcgtccc caaccggggc ggccatcatc
    35221 gcttcgagca tggcgcggct cggtcgggta acggtatcac tgcgtaaatc aatcatggca
    35281 tgtccttatt atgacgggaa atgccaccct ttttacctta gccagttcgt tttcgccagt
    35341 tcgatcactt catcaccgcg cccgctgatg attgcgcgca gcatatacag gctgaaacct
    35401 ttggcctgtt cgagtttgat ctgcggtgga atggctaatt cttctttggc gaccaccaca
    35461 tccaccaaca ccggaccgtc gatggagaag gcgcgttgca gggcttcatc gatttcagac
    35521 gctttttcta cgcggatacc cgtaatgccg caggcttcgg caattcgggc aaagttggtg
    35581 tcgtgtagtt cggtgccgtc agtcaaatag ccaccagctt tcatctccat cgccacaaag
    35641 cccagcacgc tgttattaaa gacgataatt ttcactggca gcttcatttg cactaccgag
    35701 aggaaatcgc ccatcaacat gctaaaaccg ccatcgccgc acatggcgac cacctggcgt
    35761 tctggctcgg ttgcctgcgc gcccagcgcc tgcggcatgg cgttagccat cgaaccatgg
    35821 ttaaacgaac ctaacaggcg acgcttgccg ttcattttta gataacgtgc cgcccacacc
    35881 gttggcgtac caacgtcaca ggtgaaaata gcgtcatcgg cggcaaaatg actaatttgc
    35941 tgcgccagat attgcgggtg aatggctttc tcgctcggtt tagctaaatc gtccagccct
    36001 ttgcgggcgt cgcggtaatc ttccagcgcc ttatccagaa acttgcgttc ggttttttct
    36061 tccaccagtg gcagcaatgc gcgcagggtt gatttgatat cgccgaccag cgccatatct
    36121 accttgctat gcgcgccgat gctggctggg ttgatatcaa tctgaataat tttggcatcg
    36181 gtcgggtaga acacacggta gggaaactga gtaccgagca gcactaacgt atcggcgttc
    36241 atcatggtat ggaaacctga cgaaaagccg attaatcccg tcattccaac atcatatgga
    36301 ttatcgtatt cgacatgttc tttaccgcgc agggcatgga ctataggcgc tttaattttc
    36361 ccggcaaact caactaactc tttatgcgcc cccgcacagc cgttgccaca catcagggcg
    36421 atattgctgg aataacgcag cagttgcgcc agtttgcgta actcttcttc ttccggtgtt
    36481 actaccggct gtggcgcatg ataccagtgg gtagttgccc cttctggcgc aggttttaac
    36541 gccacatcgc ccggtaacac aacaacggaa acgccacggt taagcaccgc tttgcgcata
    36601 gcaattgcca gcacttgtgg gatctgctct gggctggaaa ccagctcgca atagtgacta
    36661 cattcgcgga atagctcttg tgggtgggtt tcctggaaat agccgctgcc aatttcgctg
    36721 gagggaatat gagcggcaat cgccagtacc ggaacgtgat tgcggtggca atcgaacagg
    36781 ccgttgatta agtgcaggtt gccgggaccg cacgatccgg cacagaccgc cagttctccg
    36841 ctaagttgtg cttcagcgcc agcggcaaat gccgccactt cttcatgccg ggtggacatc
    36901 cactcgatgg tgcccatgcg attaagactg tcactaagac cgttcagaga gtcgcccgtg
    36961 actccccaga tgcgtttcac gcctgccgat tcgagtgttt tggcgatata agctgcaacc
    37021 gtttgtttca tggttctcca tctcctgaat gtgataacgg taacaagttt agttcatctg
    37081 acggaggggg aagggatggg agaaaaagga ggcactaacg gttaaatagc ccgatgaaag
    37141 gaatatcatc gggcatcagg cgattatgcg aaaaccaaat ccccctgcgg atggcatgag
    37201 caggccagta cgtaaccttc agcgatttcg gcgtcggtca gcgtcattgt gctgctcacc
    37261 gtatattcac cggaaatcac tttcgtctta cagcagccgc aaacacccgc acggcaggcg
    37321 gcgacaaccg gaacgttatt gctttccagc gcctccagta gcgtggtgcc aaccggagcg
    37381 taaaattctc gtgccggttg cagtttggtg aatttcagac cgctggtcgc cgcttccgct
    37441 actggggtga agaatttctc tttaaagaaa cgcgtcacgc cgagcgtttt cacttcctgc
    37501 tctacccaat ccatatatgg agcagggcca caggtcatca cggtacgtga ggctaaatca
    37561 gggacacctg ccagcagttc gcgagtgaga cgaccagcga taaagccttc ggtaacgtta
    37621 ttttccgcca ccagcgttac cggatagtta cgccactcat cggcgaaaat cacatcctgc
    37681 ggcgtacgca cgttgtagat cacctgcaca tcggcctgtg gacggttctt cgccagccag
    37741 cgacgcatcg acataatcgg cgtgacgccg cagcctgccg ccagcaacag gaatttatct
    37801 tctgctttat cgtcgcaggt aaattccccc atcgcgtccg aaagccagag ataatcaccg
    37861 cgttttacat cgcgcgtcag ccaccgggag ccgacaccgt catcaatccg ccgcacagtc
    37921 agggtgatgt attcactcac gcctggcgtg gaggaaatgg tgtaagcacg cagcgtttct
    37981 gctgagtttc gcacgctgac cagcgcatat tgcccggcgc gatacgggta gtaatcgtgg
    38041 caaatcaggg aaatcgtcca cacatccggc gtttcttgcg taatgtgatg aacctgcatc
    38101 cgccacgggc attgattcgt tggcatcgtc atcgacaaac tccttacgcg ctcaacagtt
    38161 gcttcatgtc ttcttcaaca gtggtgatag aacgcaggcc gaatttctcg ttcagcaccg
    38221 ccagcaggtc tggtgtcagg aaaccaggtg cagtcgggcc ggtgacgata tttttcacac
    38281 ccagagaaag cagcgtcagc agaatgacaa tcgctttctg ttcaaaccag gagagcacca
    38341 gcgacagcgg cagatcgttg actccgcagc ccagtttctc tgccagcgtt accgccagaa
    38401 taattgctga gtaagcatcg ttacactgac cagcatctac cagacgcggc agaccttcga
    38461 tatcgccaaa ctcaagttta ttaaagcgat atttaccgca agcgagggtc aggatcaggc
    38521 agtcatccgg cacgctggtg gcgaaatcgg tgaagtagtg acgctcgccg cgtgcgccgt
    38581 cacagccgcc aagcaggaag atatgacgca gtttttcacg gctcaccaga tcaatcagcg
    38641 tatcggctgc gccaagcagt gtctggcgac caaagcccac ggtgatcagg tgcggaattt
    38701 cgctgtacgg gaagcccgcc atctgttgcg cctgggcaat gaccgcagag aaatcttcgc
    38761 catccagatg gcgcacaccc ggccagccaa cgatgctgcg ggtccagata cgatcgtcat
    38821 aagcgcctac ggttgggtcg atgatgcagt tcgaggtcat cacgatgggg cctgggaaac
    38881 gagcgaactc cacttgctga ttctgccagc cgctgccgta gttaccgacc agatgcttga
    38941 atttacgcag ctccgggtaa ccatgcgcag gcagcatttc gccgtgggtg tagacattaa
    39001 cgcccgtgcc ttcggtctgt tccagcaggt tgtagagatc tttgagatcg tgaccggaaa
    39061 tcaggatgca tttacctgcc gtcgctttga cattgacctg ggttggggtc gggtgaccgt
    39121 atttaccggt ttcgcctgcg tccagaatgc tcatcacttt gaagttcatc tggccgattt
    39181 ccattgaaca ctcaagaagc gcgttcatat cggcaggcca ggtccccagc cacgccatga
    39241 ttttatggta ctgggcataa atatcgttgt cgtattgacc gagaacatgc gcgtgttcca
    39301 tataggccgc cgcacctttc aggccataca ggcacagcag acgcaggccg agaatgtttt
    39361 cgccaatcgc cgctttatct ttgttagggg taaattctgc tgcctgacgt tgcagctcgc
    39421 cgagatcgtc gctcaccagt tgcaggtcag ccatcgggtt atcgacgcgg gcgttggcat
    39481 ctacagccag gcattgtgct ttcagcgcct cgcgcagggc aatcgcttca cgagcgtagc
    39541 cgataatacg cggagaatcg aagttaacgt tggtcagggt tgagaaaaag gcacgtggcg
    39601 caaagctgtc tacatcgtgg ttgatgatgc cgtattcacg tgctttcact gcccaggccg
    39661 aaagcccttg cagcgtcgcg atgagtaaat cctgaaggtc agaggtttcc gccgttttac
    39721 cacacatccc ctgcgcgtat gagcagccgt ttcctgccgg agtacggata gtttgttcac
    39781 attgcacaca aaacatgatc acacctttta aagttatatt taatatacat gtttaaggtt
    39841 aagacgctta acgcggggat aaaagagatt tttaatgcaa ctttaaggga gattgattta
    39901 gcgcaatttt ggcggcagag atctaccgcc agagaggtat tatgcagaga aaaaggcgat
    39961 gagaatcggc actaacagac taagaataaa accgtgaaca atcgccgccg ggaccatatc
    40021 cagcccgcct gtacgttgaa gaacgggcag ggtgaaatcc atcgacgtcg caccgcataa
    40081 gcccagtgca gtagagcggc tacggcgaat cagcccaggg attaacataa tagcaatcag
    40141 ctcacgggcc agatcattaa aaaacgccgc gctcccgatt accggaccaa aagattcggt
    40201 caataaaata ccggaaagag aataccagcc gaaaccggag gccattgcca gcgcggtatt
    40261 gatgggaaga tcaagaataa aggcgttaat taaaccacca attaatgaac tggcaaccac
    40321 caccacggcg acaatcattc cccggcgatt aaggacaatc tgctttaagg tcatgccatt
    40381 attgcgcaac tgaataccga cgaggaaaag cagcagaatt aaggtgtatt cactggcttc
    40441 ggtcgcgtgt tgtaagaaag ccagtccact cagaccaatg gcaaaaccaa tcactactac
    40501 gccgcacagt tttagcgact ccagcgccat cgcaatacgc gacgggagtt tttcttgctg
    40561 atggtggttg ctccatggca ggccgcgctc cagccacatc agggcggcaa tattacacag
    40621 taaaataacg gtagtactga cggcagaata atgcagaatt gccaacaagt tactggcgag
    40681 gttatcgaga aacgccagac tgatacccat aaaaaagaga ataaggtaaa ccatccagct
    40741 taagagctga ttaataactc ttaatgcagc tttttggcga agcggaatga ggtaacccac
    40801 aatcagggga accagaatga ttaacagccc agaaaacatg aaaacccagt ccttgcaaag
    40861 aagattcgaa atgcgcgatg acacactact gaaagcggaa ggacgagtaa agttgcaatt
    40921 aaaaggaaat gttatgcata aggatcggta gggtattcat tttcatttaa agatattctt
    40981 gcgctttaat tacaaaccgc accggtgttg gtggcgtcaa aatcgccgac ccggtagccg
    41041 taagggataa gtcgagggca ccgcgcagcg gcgattttgt tcgccagagc ccgggggtgc
    41101 agggggcggc ggcgattggc cgccccctgc acgctccttg cgccagtggc aatatgtggc
    41161 ttagctcatg aaaggagcgc aacaagatga tgaatcaaca tataacaaca tcttaaaaaa
    41221 aggcctgaca ttgcgccagg ccttctgcgt taattaatta cgcttttcca gtagagtccg
    41281 gtaaatcaga ccaccgataa taccgccgac aattggcacc acccagaaga accacagttg
    41341 ttctaatgcc cagccgccct ggaagatagc aaccgcagta ctgcgcgccg ggttaacgga
    41401 agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac caatggcaat
    41461 tggcgcaaaa ccagcaggag cgaatttgtc agttgcgccg tggatcacca gcaggaaacc
    41521 tgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc ctggtgaatg
    41581 ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg ttttaccact
    41641 ggcaattaaa tacagcagcg ccgccgcaac aataccgccg acaacctggg caattacgta
    41701 gccaacgact tcttttgccg ggaaacgtcc gccagcccat aaaccaatag tgaccgccgg
    41761 gttaaaatga ccaccagaaa tatgaccaac agcaaaggcc attgtcagaa cggtcagacc
    41821 gaacgccaac gccacgccgg caaaaccaat gcctaattcc gggaagcctg cggccagtac
    41881 ggcactacca cagccgccaa aaacaagcca gaaagtacca aaacattcag ctgctaattt
    41941 tctgaacata tccacctcaa ttaaaaattg accctgtgaa aaatatggtc gttttatagg
    42001 gtcgtcgtaa aaaagtgacg acggaaataa tgcgcggcta ttttaaaaac gaaggcaaat
    42061 cattcgccag ataaataaat ccagtaaatt tgatttaggg caacagcggg ttgccccata
    42121 tagtcatttg tctgattgac agtgtagtgc aagcgaaaga tttaatcctt taggcgtaat
    42181 aaaaaataat ttatcatgct aattatttga ttttgttgtt tttgcaggct tatcagcaag
    42241 agggagtata acgcgattat tcgctcattt ttcagacatt tgccatgctt aaatgtgatg
    42301 ccatcacgta ttagcaggcc tttcccgtta tactgccagc gtaaagaata agtcacatat
    42361 ttctggaggg gatatgattc ttgagcgcgt tgaaattgtg ggttttcgcg gtatcaaccg
    42421 tttgtcgttg atgctggaac aaaacaacgt cctgattggg gagaacgcgt ggggtaaatc
    42481 cagcttgctg gacgccttaa ccctgctgct atcgccagaa tcagatctct accattttga
    42541 gcgcgatgat ttctggttcc cgccgggaga tatcaacggg cgagaacatc atctgcatat
    42601 tattttgacc ttccgcgaat cgctgccagg ccgacatcgg gttcgccgct atcggccgct
    42661 ggaagcgtgc tggacgccat gcaccgatgg ttatcaccgt attttttatc gtctggaagg
    42721 ggagagtgcg gaagacggca gcgtgatgac actgcgcagt tttctcgata aagacggaca
    42781 tccgattgat gttgaggata ttaacgatca ggctcgtcat ctggtgcgtt taatgccggt
    42841 gctgcgcttg cgtgatgccc gttttatgcg ccgtattcgt aacggcacgg tgccaaatgt
    42901 ccccaatgtg gaagtcaccg cgcgccagct cgatttcctc gcccgtgagt tgtcctcaca
    42961 tccgcaaaat ctctctgatg gacagattcg tcagggactt tccgcaatgg tgcaactgct
    43021 tgagcattat ttttctgagc agggagctgg acaggcgcga tatcgtttaa tgcggcggcg
    43081 agccagcaat gagcaacgaa gctggcgcta tctggatatc atcaaccgga tgattgaccg
    43141 acctggaggg cgctcgtatc gggttatttt gctcggcctg tttgctactt tgttgcaggc
    43201 aaaaggcaca ttgcgactgg ataaagacgc ccgtccattg ttgctgatcg aagatccaga
    43261 aacccgtttg caccccatta tgctttcagt tgcctggcat ctgttgaacc ttctgccatt
    43321 gcagcgcatc gctactacca actcgggaga gttgctttcg ttaacgccgg tagagcatgt
    43381 ttgccgactg gtacgtgagt cctctcgcgt tgccgcctgg cgtctgggac cgagtggctt
    43441 gagtaccgaa gatagccgac gcatctcctt tcacattcgt tttaaccgtc cgtcatcgct
    43501 gtttgcccgt tgttggctgc tggtggaagg ggaaacggaa acctgggtta tcaatgaact
    43561 ggcgcgtcag tgcggacatc attttgatgc cgaagggatc aaggtcattg agtttgccca
    43621 gtccgggcta aagccactgg ttaaatttgc ccggcgaatg gggattgaat ggcatgtact
    43681 ggtcgatggc gatgaagcag ggaagaaata tgccgctacg gtacgcagcc tgttgaataa
    43741 tgatcgggaa gccgaacgag aacatttaac ggcgttaccg gcgctggata tggaacattt
    43801 tatgtatcgc cagggatttt ccgatgtgtt ccaccgcgtg gcgcaaatcc cggaaaatat
    43861 accgatgaat ctgcgcaaaa ttatctcgaa agcgatccat cgctcttcca aacccgatct
    43921 tgccattgaa gtggcaatgg aggccggacg tcgtggtgtg gactccgtac cgacgctgct
    43981 gaaaaaaatg ttctcacgcg tgctgtggct ggcgcgcggt cgggccgact aactacaaaa
    44041 catcgtggcc atttgtggct gaatagcgtc gagcatctca tagcgccgac ggtattcagc
    44101 ccgtttttta ctggcgattt cggcaatctc ttttcgtgct atctgtgctg gaaggcggta
    44161 atggcgttca gcatcacata cgccgccaac cgattcccag aaagcgttgt aatcggcatg
    44221 gatcttgcct tctttatcgc gataacgcag gctgcggtaa atatgcgttt cattgctgac
    44281 ggcaataatc tgctctacct gcaaacgttg ggcaaacaga caggccgctt ccatcacgag
    44341 gcgtttggga aatagcccgt ggcaggcttt cgtcgcattc tggatttcct ggtgtggaat
    44401 ttcccatttt gcgccttgca gtccgccaat aaacatcgtc cttttcccct gatattcaca
    44461 cagggtaaaa gtgagctctg ccagaggaat accttcgctg ttgcggaaca ggatggtgct
    44521 gtcaccttct ttatccattg agatcatcat ggtcagctca agcgtgaact gctcgccgtt
    44581 tttgccttcc agcttcgcca gttgcagccc gggggtattc aaatataagc tgaattcttc
    44641 cgccgacata cacccgcgga gtaacgcata atggtaacgt aacgcctcca gcaattgctt
    44701 acggctaaga ttcgccgcaa ggtaagggcg atgtagacgt acaggcagtc gcggctggcg
    44761 cgttaacaac acattgagat taggccagtg ggaaagttcg ttcatccact caacgcttaa
    44821 acgcggcata atcaacgagc gcagcaaaaa tttctggcga aaactacggc gatgccagaa
    44881 tttacccggc cgacactgtc ctcgtgccag actaagaaaa agtgacaggc tgctgagaga
    44941 ttcagatggc gtaaaggtcc gttcagttag ctgcgacata ttcatgaaat caatggttat
    45001 acatgacgtc gatttaacca ttgcgtatct taaccaaaca tcaatagtgt gattattaac
    45061 gtaaatttta gggttttgtt gatatttcgt tgaagttaat gacccggatt ggcatatgga
    45121 gtattcagaa tatttatgaa aaagcggaaa accgtgaaga agcgttacgt tattgcgatg
    45181 gtgatagtca tcgccgggct gattacgtta tggagaattc ttaacgcacc cgtgccgact
    45241 tatcagacgc tgattgtgcg ccccggtgat ttacagcaaa gcgtgctggc gaccggaaag
    45301 ctggacgcgc tgcgtaaggt tgacgtgggc gcgcaggtca gcggtcagtt gaaaacgctg
    45361 tcggtggcga ttggcgataa agtaaaaaaa gaccagcttt taggggttat tgatcctgaa
    45421 caggctgaaa accagatcaa ggaggtggaa gcaacgctga tggagctacg cgcgcagcgg
    45481 cagcaggcgg aagcggagct gaaactggcg cgggtgacgt attcccgtca gcaacgtctt
    45541 gcacaaacgc aagcggtttc acagcaggat ctcgataacg ccgcgacgga gatggctgtg
    45601 aaacaggcgc aaattggcac cattgacgcg caaatcaagc gcaatcaggc ctctctcgat
    45661 acggctaaaa ccaatctcga ttacacccgc atcgttgccc cgatggccgg ggaagtcacg
    45721 caaatcacca ctctgcaagg ccagacggtg attgccgcac aacaagcacc gaacattctg
    45781 acgctggcag atatgagcac catgctggta aaagcgcagg tttctgaagc ggatgtaatc
    45841 cacctgaagc cggggcaaaa agcctggttt acggtgcttg gcgatccact gacgcgctac
    45901 gaggggcaaa tcaaggatgt gctaccgacg ccggaaaaga ttaacgacgc tattttctat
    45961 tacgcccgtt ttgaagtacc caaccccaat ggtttactgc ggctggatat gaccgcgcaa
    46021 gtgcatattc agctcaccga tgtgaaaaat gtgctgacga tccctctgtc ggcgttaggc
    46081 gatccggttg gcgataatcg ttataaagtc aaattgttgc gtaatggtga aacacgcgag
    46141 cgtgaagtga cgattggcgc acgtaacgat accgatgttg agattgtcaa agggctggaa
    46201 gcgggcgatg aagtggtgat tggtgaggcc aaaccaggag ctgcgcaatg acgcctttgc
    46261 tcgaattaaa ggatattcgt cgcagctatc ctgccggtga tgagcaggtt gaggtgctga
    46321 agggcatcag cctcgatatt tatgccggtg agatggtagc gattgtcggt gcttcgggtt
    46381 ctggtaaatc gaccctgatg aatattctcg gctgcctgga taaggccacc agcggcacct
    46441 atcgcgtcgc cggtcaggat gttgccacgc tggacgccga tgcgctggcg caactgcgcc
    46501 gcgagcattt cggctttatt ttccagcgtt accatttgct ttcgcattta accgccgagc
    46561 agaacgttga agtacccgcc gtctatgccg gtcttgagtg gaaacagcgg ctgcttcgcg
    46621 cccaggagtt actgcaacga ctggggctgg aagaccgtac agagtattac ccggcacagc
    46681 tttcgggtgg ccagcaacag cgcgtcagta tcgcgcgggc attgatgaac ggcggtcagg
    46741 taattcttgc cgatgaaccg accggcgcac tggacagcca ttctggcgaa gaggtgatgg
    46801 cgatcctgca tcagctgcgc gatcgcgggc atacggtgat tatcgtcaca cacgatccgc
    46861 aggttgctgc tcaggccgag cgggtgatcg aaattcgcga cggcgaaatt gtgcgcaatc
    46921 ctcccgccat tgaaaaagtg aatgctactg gcggaacgga acctgttgtc aacacggcgt
    46981 ctggctggcg gcagtttgtc agcggtttta acgaggcgct gacgatggca tggcgggcgc
    47041 tggcagcgaa taaaatgcgt actttactga ccatgctggg gattattatc ggtattgcgt
    47101 cggtggtttc cattgtcgtg gtgggcgacg ccgccaaaca aatggtgctg gcggatattc
    47161 gttctattgg tacgaatact attgatgtct atcccgggaa agattttggc gatgacgatc
    47221 cgcaatatca gcaggcgctg aagtacgacg acttaatcgc catccaaaaa caaccgtggg
    47281 tcgcctcagc cacacctgcc gtctcgcaaa acctgcgcct gcgttataac aatgttgatg
    47341 tggctgccag tgccaatggc gtgagcggcg attattttaa tgtctatggc atgactttca
    47401 gtgaagggaa cacctttaat caggagcagc tgaacggtcg tgcgcaggtc gtggttctcg
    47461 acagtaatac tcgccgccag cttttccccc ataaagcaga tgtggttggc gaggtgattc
    47521 tggttggcaa tatgcccgcc agagtcattg gtgtggcgga agaaaaacag tcgatgtttg
    47581 gtagcagtaa agtgctgcgt gtctggctgc catacagcac gatgtccggg cgagttatgg
    47641 gtcagtcgtg gctgaactcc ataactgtca gggtgaaaga aggctttgac agcgctgagg
    47701 cggaacagca actcacgcgt ttactttcac tgcgccacgg taagaaggat ttctttacct
    47761 ggaacatgga tggcgtcttg aaaaccgttg aaaagaccac acgtacttta caactgtttc
    47821 tgacgctggt ggcggtgatt tcgctggtgg tgggcggtat tggtgtaatg aatattatgc
    47881 tggtgtcagt gaccgagcgg acgcgggaaa ttggcattcg catggctgta ggtgcgcgag
    47941 caagcgatgt tttgcaacag tttctgatcg aagccgtact ggtttgcctg gttggtggcg
    48001 ctttgggaat aacactgtca ctgttaattg ctttcacctt gcagcttttc ttacccggct
    48061 gggagattgg tttttcaccg ttagcgctgc tgctggcgtt tctctgctcg acggtcaccg
    48121 ggattttatt tggctggtta cccgcacgaa atgcggcacg actggatcca gtagatgctc
    48181 tggcacggga gtaatttttg agataaaaat gccagccgat cgggctggca ttttgccttt
    48241 aggatgtaca caatgagaca gaagagctat gcgactgccg cttctacttc gacgggcaca
    48301 ataacactgg cgtgattgcc ttttggcccc tggtggacat caaactgaac ggattgtcca
    48361 gcttttagcg ttctgtaacc atccatctga atggtggaat aatgagcgaa aatatcttcg
    48421 ccgccgcctt cagggcagat gaaaccaaac cctttggcat tgttgaacca cttaacagta
    48481 cccttttcca tgcttcgaca tccttcgcaa atcttataca agtaagatgg aataaaccgg
    48541 ggtcagagag ggggctgttc aaaacctcgc caactctaga aatacaattt agagaattag
    48601 ggcgagccgt caagcatttg acaggggcca gggggcaggt atgaatcaaa aatttgaagc
    48661 agttaacgct attgacagga atgtgacaga tgtcgctgat gccaacgata gatgatagtt
    48721 atctatcatg tggagtagat tggtcaggca aataagctct tgtcagcggc agggcgttct
    48781 gccgataacc gtaaccgaag atgataactg acaatgggta aaacgaacga ctggctggac
    48841 tttgatcaac tggcggaaga aaaagttcgc gacgcgctaa aaccgccatc tatgtataaa
    48901 gtgatattag tcaatgatga ttatactccg atggagtttg ttattgacgt gttacaaaaa
    48961 ttcttttctt atgatgtaga acgtgcaacg caattgatgc tcgctgttca ctaccagggg
    49021 aaggccattt gcggagtctt taccgctgag gttgcagaaa ccaaagtggc gatggtgaac
    49081 aagtacgcga gggagaatga gcatccattg ctgtgtacgc tagaaaaagc ctgaatgcag
    49141 gcataaaaat tgggggaggt gcctatgctc aatcaagaac tggaactcag tttaaatatg
    49201 gctttcgcca gagcgcgcga gcaccgtcat gagtttatga ccgtcgagca cttgttactg
    49261 gcgctgctca gtaacccatc tgcccgggag gcgctggaag cgtgttctgt agatttggtt
    49321 gcgctccgtc aggaactgga agcctttatt gaacaaacca cacccgttct gcctgccagt
    49381 gaagaggagc gcgacacaca gccgacgttg agttttcagc gtgtactgca acgtgcggtc
    49441 ttccatgtcc agtcctccgg tcgcaatgag gttaccggtg caaacgttct ggtcgctatc
    49501 tttagcgaac aggagtcgca ggcggcatat ctgttgcgca aacatgaagt cagccgtctc
    49561 gatgtggtga actttatctc tcatggcacg cgtaaagacg agccgacaca gtcttctgat
    49621 cctggcagcc agccaaacag cgaagaacaa gctggtgggg aggaacgtat ggagaatttc
    49681 acgacgaacc tgaatcagct tgcgcgagtg ggcggaatcg acccactaat tggtcgtgag
    49741 aaggagctgg agcgtgctat tcaggttctc tgccgtcgcc gtaaaaacaa cccgctgctg
    49801 gtgggggagt ctggtgtcgg taaaaccgcg attgcggagg ggcttgcatg gcgaattgtt
    49861 cagggcgatg tgccggaagt gatggctgac tgtacgattt actctctcga tatcggttcc
    49921 ctgttagcgg gcacaaaata tcgcggcgac tttgaaaaac gttttaaagc gttgctcaag
    49981 cagctggagc aggacactaa cagcatcctg tttattgatg agatccacac cattatcggt
    50041 gcgggtgcag cgtctggtgg ccaggtcgat gcggctaacc tgattaaacc gttgctctcc
    50101 agcggtaaaa tccgcgtaat tggttcgaca acctatcagg agttcagcaa cattttcgag
    50161 aaagaccgtg ctctggcgcg tcgcttccag aaaattgata ttactgaacc gtcaatcgaa
    50221 gaaactgttc aaatcatcaa tggcctgaaa ccgaagtatg aagcgcacca cgacgtgcgt
    50281 tataccgcaa aagcggtgcg tgcggcggta gagctggcgg tgaaatacat taacgatcgt
    50341 catctgccgg ataaagcgat tgatgttatc gacgaagcgg gcgctcgcgc acgtctgatg
    50401 ccggtaagca aacgcaagaa aaccgttaat gtggcggata ttgaatccgt ggtggcccgt
    50461 attgcacgca ttccagagaa gagtgtttct cagagtgacc gcgataccct gaaaaacctc
    50521 ggcgatcgcc tgaaaatgct ggtcttcggt caggataaag ccattgaggc gctgactgaa
    50581 gccattaaga tggcgcgtgc aggtttaggt cacgaacata aaccggtcgg ttcgttcctg
    50641 tttgccggcc ctactggggt cgggaaaaca gaggtgacgg tacagctttc gaaagcgttg
    50701 ggcattgagc ttctgcgctt tgatatgtcc gagtatatgg aacgccatac cgtcagccgt
    50761 ctgattggtg cgcctccggg atacgttggt tttgatcagg gcggtttgct gactgatgcg
    50821 gtcatcaagc atccacatgc ggtgctgctg ctggacgaaa tcgagaaagc gcacccggac
    50881 gtgttcaata ttctgttgca ggtgatggat aacggtacgc tgaccgataa caacggacgc
    50941 aaagcggact tccgtaacgt ggtgctggtg atgaccacta atgccggggt acgggaaact
    51001 gagcgcaaat ccattggtct tatccaccag gataacagca ccgatgcgat ggaagagatc
    51061 aagaagatct ttacaccgga attccgtaac cgtctcgaca acattatctg gttcgatcat
    51121 ctgtcaaccg acgtgatcca tcaggtggtg gataaattca tcgtcgagtt gcaggttcag
    51181 ttggatcaga aaggtgtttc tctggaagtg agccaggaag cgcgtaactg gctggccgag
    51241 aaaggttacg accgggcaat gggcgcacgt ccgatggcgc gtgtcatcca ggacaacctg
    51301 aaaaaaccgc tcgccaacga actgctgttt ggttcgctgg tggacggcgg tcaggtcacc
    51361 gtcgcgctgg ataaagagaa aaatgagctg acttacggat tccagagtgc acaaaagcac
    51421 aaggcggaag cagcgcatta atctgattgt ctggtaggtt ggtgaagtcc gtaatctcgt
    51481 caggggttac ggacttttta tttatggggg gaggaggttc agaccctttt tttgatgatg
    51541 atggtaagtt attgataatt agtgctgcgg gtaggtaagg ataaaaaagg gtggcagcag
    51601 gagattgaga tggttttgct ttattaacaa cgggctaaac gtgtagtatt tgagttcact
    51661 gccgtacagg cagcttagaa attgccgcgg atcctgtctg ccaataatga caagttcact
    51721 gccgtacagg cagataaaat gcgaaaaaaa agcccgtact ttcgtacgag ctcttcttta
    51781 aatatggcgg tgaggggggg attcgaaccc ccgatacgtt gccgtataca cactttccag
    51841 gcgtgctcct tcagccactc ggacacctca ccaaattgtt tttttgcctg acctcatggg
    51901 gggcaacggg gcgctactat agggagttgg aataaaacgg tcaagaagaa tttttatgat
    51961 aattattgtt tgctcatact gtaaacaagt tgtgcagtat atctacatcg agacaagtta
    52021 cggacttata cttctaaagt actccataca tatcacaaaa taaaaaggcc ggctaaaccg
    52081 accttttact cattctttct cttcgcccat caggcggtaa aacaatcagc gactacggaa
    52141 gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca acagtcactt tgtcgcccgt
    52201 caggatgcgg atgtagtttt tgcgcatttt accggagatg tgtgcagtaa ccacgtgacc
    52261 gttttctaac tctacgcgga acatggtatt aggcaacgtt tcaagaacgg taccttgcat
    52321 ttcaatattg tcttctttgg ccatctaatc ctctggggta tcactaccgt aatttgaacc
    52381 ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt gcgcgctaaa tcgcaacaaa
    52441 caggttcggc acattactcc gaaaacacac ggctaagccg caccaaaagc gcaacgtata
    52501 agggagcggt gagataaacg atgggcgtta cctgacgcga aaaattcctt atcggcagcg
    52561 gggtaatgag cgtaaccaac tctgcgaccg caattataac actctgggga gaaatgtgcc
    52621 gaaaacattc attcttgtgg tgaaaacaag catcgaggta cccagaaatt attcgacaat
    52681 cgtccgaggc gcatttgatt gagataatta aggtaatccc ggcggggaat ttcgcaggca
    52741 ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga taagtttacc gccatgacgg
    52801 ataaattcat cacagaagac cagaagcgcc gttttggacg cattttccat ccggctgaac
    52861 atggactcgc cacaaaatag tgttccctgg gccacgccgt acataccgcc gacaagctca
    52921 tcttcacgcc agacttcaat ggagtgggca tgaccgagtt cgtgaaggcg atggtaggct
    52981 tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat cgctggcaca gccttcgatg
    53041 acctgaccaa aagcgtaatt catcgtgaca cgatagggtg agcgtttatg aaatcgcttc
    53101 atactacggc tgatatgcag tgattctggc cataacaccg cgcggggatc gggcgaccac
    53161 cagaggatgg ggtcgccagg agaaaaccac gggaaaatac cacgctgata agccattaat
    53221 aggcgcgcag ggctaagatc gccccccagt gccagcaggc cgttaggctc acgtaatgcg
    53281 ccttccgggg aagggaaggc tattgaatgg cgagaaagct gaaccaggcg catgaccgca
    53341 aaactccacg caagtcggat cgttcaataa tagcttacaa accctgctta aactggtaat
    53401 aacgcccctg tctggcaagc agttctgcgt gagtaccttg ctcaataatt tgcccgttgt
    53461 ccatcactat tatttgttgg aaacgagaga gcccgcgaag tcgatgggtg accattaaca
    53521 ccgttttctc gcacatcatt tctgcaagca attcaaggat ctgactttcg gttgtggcat
    53581 ctaagccttc tgtaggttca tccagcaata ccagcggcgc atcatgtaac agcgcacggg
    53641 cgatagccag acggcgcagt tcaccaccgg agagctggcg tccgccttca cctaaccaac
    53701 tgttgagacc tgcatcctcg agcagcttct ccagaccaac gcgacgcaag atctccgcca
    53761 gagcctcatc actactacca ggcgaggcga gtaacagatt atcacgcagc gtggcgctaa
    53821 acagatgcac tcgctgggga acaacgctga tggtctgtcg tagagccgct tcattcaggc
    53881 tggctatggg actatcgtta agcaaaatct cgccctgttg cgggtcccat gcgcgggtca
    53941 gcagttgtaa cagtgttgac ttgccgcatc cggttcgccc gagaatcgca atatgttccc
    54001 cggcgtttac ctgaagataa atccctttaa gtgcctgttg ggattgctcc ggataagtga
    54061 actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac acgagtttgg gtatcaggga
    54121 aggtgacttt cggtttttga tccgttaaat cagtgatccg tacggcagag gcaatgactt
    54181 gccccagatg ctgaaatgca cccgttactg gtgccagcgc ttcaaacgcg gctaacgcgc
    54241 agaagacaaa cagggcaatt aacgcgccgg gttgagcatt gccgccaacg ccgccagacg
    54301 ccatccacag catcaggatc accgctaacg cgccaatgag cagcattatc gcttgcgaca
    54361 atgcggttag ttcagattga cggcgttgcg cttccagcca ttgaatttct gtattctcta
    54421 attgcgtgcg ataacgatcg ctggcaccaa aaatggtcag ctcagcttgc ccttgcagcc
    54481 aggccgtcag ttgttggcga tactgcccgc gaagatgagt cagattttgc ccggtgcttt
    54541 ttcccgcacg ataaaacagt ggtggcatca ggaaaagcgt cagtaacata atgccgccca
    54601 gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat tgtcaccacc ataatcacca
    54661 caaaagcgcc taccagcggc gagataacgc gcaggtaaag atgatcgagc gtatcaacat
    54721 ccgccaccac gcggttgagt aattcgccct gacgatagcg cgccagtcct gcaggggaga
    54781 ggggcagtaa tttgctgaag gtgtaaatgc gcagatgctg caacacgcgg aaagtcgcat
    54841 cgtgacttac caggcgttca aaatagcgcc cggcagtacg ggtgattgct gcgccacgca
    54901 cgcccgcagc gggtagcata tagttgaagc tgtacagtcc ggcaaccccc gcaaccgctg
    54961 aggcggagag gaaccagccc gaaagtgtca acagaccgat actggcgagc agcgtgacaa
    55021 ttgccagcac gataccaaga cttaacatcc atttatgacg tttatacagt gccagatagg
    55081 gtagcaaagc gcgcatttaa atctcctcct gacgatgggc cagtaatgtg gcgaatgggc
    55141 caccagccac acttaattcc gcgtaacgtc cttgctcaat aatctgacca tcctgcatta
    55201 cccaaatgac atcccagtca gcaagatctt ctaactggtg ggtgaccatt aacgttgtct
    55261 ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg ttcactgtga gcatcaaggc
    55321 tggcagcggg ttcatccaac agtaatagcg aacagggatt tagtaacgca cgggccaccg
    55381 ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg gtcgccaaca ggcgtatcaa
    55441 cgccttgtgg caggagcggt agaaactcac tgacccaggc gttatccagc gctgtttgta
    55501 actcttgttc gctggcatca ggtcgcgcca gtagtacgtt atcccgtaat gttgctgccg
    55561 gtaattgtgg gttttgccca acccaggaga gatgtttacg ccatgattcc ggtgataaat
    55621 cgcgtaattc tatcccgttg attcgtagcg atccctgata tgagagaaaa ccagaaagcg
    55681 cgttcaacag tgaactttta cctgaaccgc tgcgaccaac caacactgct cgttggcctg
    55741 ctggcaaagt aaaattcagc ggtccggcca gcgttttacc ttccggcgac gtgataaaca
    55801 gatcctcggc ttcaatggtc accggatcgg tcgatgctaa ttccgcttca ccgcgctgcg
    55861 gatgggctag cggggtttcc ataaacgttt tcaaactgtc agctgcacca acagcctggg
    55921 ctttagcatg ataaaacgta ccgagatcgc gtaatggctg gaaaaactct ggcgcaagga
    55981 tcagggccag aaaacccgca gccagcgtca cgccagtatc gtagtgacca aaatccagct
    56041 cgccgaggta ggaaaaacca aagtagaccg ccaccagagc aatcgacagc gaggtaaaaa
    56101 attcgagaat gccggaggat aaaaacgcca gccgtagcac ttccattgtc cgttggcgga
    56161 aatcttccga agcagaacga atactttcaa tttcagcttc accacgacca aaaatacgca
    56221 atgtttccat gccgcgcagg cgatcgagga aatgcccact taagcgagca agagcgagaa
    56281 agttacgtcg gttagcatcg gcagccccca ttccaaccag cgccataaat aacggaatta
    56341 gcggtgcagt gcccagcaga atgagcgccg cggcccagtt agaggggaag atagccacca
    56401 caatcagcag cggcaccgac actgccagcg ccatttgcgg caggtagcgt gcatagtaat
    56461 catgcatatc gtcaatttgc tcgagtacca gcgtcgccca gctccccgca ggtttaccct
    56521 gaatccacgc tggccctgct tgttgcagac ggtcgagaac ctgacggcgg atggcaaagc
    56581 ggatatgctg cccggcgtga taacccaccc gttcgcgtaa ccagaccacc catgcgcgca
    56641 gtacaaaggt cagaaacagt aacgtaaagg gaagcagcag ggcttcacgg ggaatattct
    56701 ccataatcat atgttgcaga attcgcgcca tgaaccaggc ctgggcaatg atcaatatgc
    56761 cgctcacaaa gcccagcaga cgagaaatat tcagccaacg ttgggagatg acgctttgct
    56821 gttttaacca gcgggttaat tctttttgac gggatttatt cattgcacgc ttagcaggtg
    56881 agttatcaga attatttgca gagcaatgtt acaacgggga aaaaataaag gcgacccata
    56941 gtcgcatggt gtcgccttct ttacttttgt tactgatttg taaaattatt ttgcgtcagc
    57001 taaaccgtcg aggtagcgtt ccgcatcaag agctgccatg cagcctgtac cagcagatgt
    57061 aatggcctgg cgataaatgt gatccatcac gtcgcctgcg gcaaagacgc cagggatgct
    57121 ggtctgggtg gcattaccat gaatacccga ctgtactttg atgtagccgt tttccagttc
    57181 cagctgccct tcgaaaatgg cagtattcgg gctgtgaccg atagcaacaa acagaccggc
    57241 aacgtcgagt gactcgatat tatcggtgtt ttgcgtatcg cgcagacgaa cgccagtgac
    57301 gcccatctga tcgccggtca cttcttccag cgtacggttg gtgtgcagaa taatgttgcc
    57361 gttctccact ttatccatca gacgcttaat gaggattttt tccgcacgga aaccatcacg
    57421 gcggtgaatc agatgaactt ccgaagcgat gttagacaga tacagcgcct cttcaaccgc
    57481 ggtattgccg ccgccgatga ccgcaacttt ctggttgcga tagaagaaac cgtcgcaggt
    57541 tgcacaagca gaaactccac ggcctttaaa ggcttcttca gagggcaggc cgagatagcg
    57601 tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg tattcgccgc tatcgccagt
    57661 cagacggaac ggacgattct gcaaatcaac tttgttgatg tgatcaaaga tgatttcagt
    57721 ttcaaatttg gtggcatgtt cgtgcatgcg ctccattaat aacggaccgg tcagatcgtt
    57781 tggatcgcca ggccagtttt ccacttccgt ggtggtggtc agttggccgc ctttttccat
    57841 gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg tagacagcag cggtgtatcc
    57901 cgccgggcct gaacccagga taagcagttt actgtgtttg gtcgtgccca tgagatcccc
    57961 atagttgttg gcagacaatg gcaggattgt agggaattta cagacgtaaa aaaagagtat
    58021 gacgattttg ttaacaattt gtgcaatcgg cagcatcgat aagcaggtca aattctcacg
    58081 tcattatcac ctctgctact taaatttccc gctttataag ccgattaaat gatgaataaa
    58141 cgcccctgtt aatgaatatc tggcatgttg tactaaaaat cgatgttttg ctttgacaat
    58201 cccctggtgt tttgcgaaaa cattcgagga agaaaaaaaa cagtattctt atatgcgcat
    58261 aaccatgcat gtaaatacca tgtttaccgt gctagtgaaa tctacgtatg gcgtggacag
    58321 acgccattcg tgatgtcgat agctgccaca aggcaacggt cttctcaccg tagacccagg
    58381 cattgcgcgc cgtgaatctt catgatttcg gtctatcgtg acgggtagcg actctgaaca
    58441 gtgatgtttc agggtcagac aggagtaggg aaggaataca gagagacaat aataatggta
    58501 gatagcaaga agcgccctgg caaagatctc gaccgtatcg atcgtaacat tcttaatgag
    58561 ttgcaaaagg atgggcgtat ttctaacgtc gagctttcta aacgtgtggg actttcccca
    58621 acgccgtgcc ttgagcgtgt gcgtcggctg gaaagacaag ggtttattca gggctatacg
    58681 gcgctgctga acccccatta tctggatgca tcacttctgg tattcgttga gattactctg
    58741 aatcgtggcg caccggatgt gtttgaacaa ttcaataccg ctgtacaaaa acttgaagaa
    58801 attcaggagt gtcatttagt atccggtgat ttcgactacc tgttgaaaac acgcgtgccg
    58861 gatatgtctg cttaccgtaa gttactgggg gaaaccctgc tgcgtctgcc tggcgtcaat
    58921 gacacacgga catacgttgt tatggaagaa gtcaagcaga gtaatcgtct ggttattaag
    58981 acgcgctaac acggaacagg tgcaaaatcg gcgtattttg attacactcc tgttaatcca
    59041 tacagcaaca gtactggggt aacctggtac tgttgtccgt tttagcatcg ggcaggaaaa
    59101 gcctgtaacc tggagagcct ttcttgagcc aggaatacac tgaagacaaa gaagtcacat
    59161 tgacaaagtt aagtagcggc cgccgccttc tggaggcgtt gctgatcctt attgtcctgt
    59221 ttgccgtctg gttgatggct gccttactaa gctttaaccc ttcggacccc agctggtcgc
    59281 aaacggcctg gcatgaacct atccataatt taggtgggat gcccggtgcg tggctggcag
    59341 atacgctgtt ctttattttt ggcgtgatgg cttacaccat tcccgtcatt attgtcggcg
    59401 gttgttggtt tgcctggcgt catcagtcca gcgacgaata cattgattat tttgccgttt
    59461 cgctacgcat cattggcgtt ttggcgctca tccttacctc ctgtggtctg gcggcaatca
    59521 acgctgacga tatctggtat tttgcctccg gtggcgtcat tggcagctta ctaagcacta
    59581 cgctacaacc actgctacac agtagcgggg gaactattgc gctgctctgc gtttgggcag
    59641 cgggcctgac gctgttcacc ggttggtcat gggtgaccat tgctgaaaaa ctcggcggct
    59701 ggattttaaa tattcttacc ttcgccagta atcgtacccg tcgcgatgat acctgggtcg
    59761 atgaagatga gtatgaagac gacgaagagt atgaagatga aaatcacggc aaacagcatg
    59821 aatcacgccg tgcccgtatt cttcgcggcg cgctagcgcg tcgtaaacgg ttggcggaaa
    59881 aattcattaa tccgatgggg cggcaaacag acgctgcgtt gttctccggt aagcggatgg
    59941 atgatgacga agagattacc tacactgcac gcggtgtggc tgccgaccct gacgacgtcc
    60001 tcttttcggg caatcgtgca acgcagccag aatatgacga gtacgatcca ttattaaacg
    60061 gtgcgccaat taccgaacct gttgctgtag cagctgctgc taccacggcg acacaaagct
    60121 gggctgcacc ggttgaacct gtgactcaga cgccgccagt tgcctctgtt gatgttccac
    60181 ctgcgcaatc tacagtagcc tggcagcctg taccggggcc gcaaacggga gaaccggtta
    60241 ttgctcctgc accggaaggt tacccacagc agccacaata tgcgcagcct gcagtgcaat
    60301 ataatgagcc gctgcaacaa ccagtacagc cacagcagcc gtattatgca cctgcagctg
    60361 aacaacctgc gcaacaacct tactacgctc ctgcggcgga acaacctgtt cagcaaccgt
    60421 attacgcaac tgcggcggaa cagcctgcgc aacagccgta ttacgcccct gcgccagaac
    60481 aagcggtggc aggtaacgcc tggcaagccg aagagcagca atccactttt gctccacagt
    60541 ctacatacca gactgagcaa acttatcagc agccagccgc tcaggaaccg ttgtaccaac
    60601 agccgcaacc tgttgaacag cagcctgttg tggagcctga acccgttgta gaagagacaa
    60661 aacccacgcg tccgccgctt tactactttg aagaagtgga agagaagcga gcccgtgaac
    60721 gcgaacaact tgcggcctgg tatcaaccga ttccagaacc tgttaaagaa ccagaaccga
    60781 tcaaatcttc gctaaaagca ccttctgttg cagcagtacc tccagtggaa gccgctgccg
    60841 ccgtttctcc gctggcatct ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa
    60901 ccgttgccgc gccagtcttc agtctggcaa atagcggtgg accgcgtccg caggtcaaag
    60961 aggggattgg cccgcagttg ccacgaccga aacgcatccg cgtgccaact cgtcgtgaac
    61021 tggcgtctta cggtattaag ctgccctcac agcgtgcggc agaagaaaaa gcccgtgaag
    61081 cccagcgtaa tcagtacgat tctggcgatc agtacaacga tgatgaaatc gatgcgatgc
    61141 agcaggatga actggcacgt cagttcgccc agacgcagca gcaacgctat ggcgaacagt
    61201 atcaacatga tgtgcccgtg aacacagaag atgcagatgc tgcggcagag gcagaactgg
    61261 ctcgtcagtt tgctcaaact caacaacaac gttattccgg cgaacaaccg gctggggcga
    61321 atccgttctc gctggatgat tttgaatttt cgccaatgaa agcgttgctg gatgatggtc
    61381 cacacgaacc gctgtttacg ccaattgttg aacctgtaca gcagccgcaa caaccggtag
    61441 caccgcagca gcaatatcag cagccgcaac aaccggttgc accgcagccg cagtatcagc
    61501 agccgcaaca accggttgca ccgcagcagc aatatcagca gccacaacaa ccggttgcac
    61561 agcagccgca gtatcagcag ccacaacagc cggttacaca gcagccgcag tatcagcagc
    61621 cgcagcagcc ggttgtacca cagccgcaat atcagcagcc gcaacagcca gttgcgccac
    61681 agccgcagga taccctgctt catccgctgt tgatgcgtaa tggcgacagc cgtccgttgc
    61741 ataaaccgac gacgccactg ccttctctgg atttgctgac accgccgccg agcgaagtgg
    61801 agccggtaga tacctttgcg cttgaacaaa tggcgcgcct ggtagaagcg cgtctggctg
    61861 atttccgtat taaagccgat gttgtcaatt actctccggg gccagttatc actcgctttg
    61921 aattgaacct ggcaccgggc gtaaaagcgg cgcgcatttc taacttgtca cgtgaccttg
    61981 cccgttcact ttcgacggtg gcggtgcgtg tcgttgaagt tattcctggc aaaccctatg
    62041 taggtctgga gttaccgaat aaaaaacgac aaaccgttta tctgcgcgag gttttggata
    62101 acgccaaatt ccgcgataat ccgtcgccat taaccgtggt gctgggtaaa gatatcgccg
    62161 gtgagccggt ggttgccgat ctggcgaaaa tgccgcactt gttggttgcg gggactaccg
    62221 gttccggtaa gtctgtcggg gtgaacgcga tgatcctgag catgctttat aaagcacagc
    62281 cagaagatgt gcgtttcatc atgatcgacc cgaaaatgct ggagctttcg gtttatgaag
    62341 gcattccgca cctgttaacg gaagttgtta ctgatatgaa agatgccgcc aacgcgctgc
    62401 gctggtgtgt taacgagatg gagcgtcgtt ataaactgat gtctgcgctg ggtgtgcgta
    62461 atctggcggg ttataacgaa aaaattgctg aagccgatcg catgatgcgt ccgattccag
    62521 acccgtactg gaagccgggt gatagtatgg atgcgcagca tccggtgctg aaaaaagaac
    62581 cgtacatagt ggtgttggtg gacgaatttg ccgacctgat gatgacggta ggtaaaaaag
    62641 tggaagagct gatagcacgt ctggcgcaaa aagctcgtgc cgcgggtatc cacctcgtac
    62701 tggcaacgca gcgtccatcg gttgatgtta ttactggtct gattaaagcg aatattccga
    62761 cccgtatcgc ctttaccgta tccagtaaga ttgactcacg taccattctt gatcaggctg
    62821 gtgcggaatc actgctgggt atgggggata tgctctactc tgggccgaac tccactttgc
    62881 cggtacgtgt ccatggtgct tttgttcgcg atcaagaagt tcatgccgtg gtgcaggact
    62941 ggaaagcgcg tggtcgccca cagtatgttg atggcatcac ctccgacagc gaaagcgaag
    63001 gtggcgcggg cggtttcgat ggcgctgaag agctggatcc gttgttcgat caggcggtgc
    63061 agtttgtcac tgaaaaacgc aaagcatcaa tttctggcgt acagcgtcag ttccgcattg
    63121 gttataaccg tgcagcgcgt attatcgaac agatggaagc gcaggggatt gtcagcgaac
    63181 aggggcacaa cggtaatcgt gaagtgctgg ccccaccgcc gtttgactaa ttaatgcatc
    63241 gtaggccgga taaggcgcgg tagcgtcgca tccggcaatc tggtaactga aaattcagta
    63301 ttttcttctt tcctcaagct gattattagc ctggaataga gagtagaggg agctcccggt
    63361 cgggagtgac gtaatttgag gaataatgat gaaaaaaatt gccatcacct gtgcattact
    63421 ctcaagctta gtagcaagca gcgtttgggc tgatgccgca agcgatctga aaagccgcct
    63481 ggataaagtc agcagcttcc acgccagctt cacacaaaaa gtgactgacg gtagcggcgc
    63541 ggcggtgcag gaaggtcagg gcgatctgtg ggtgaaacgt ccaaacttat tcaactggca
    63601 tatgactcaa cctgatgaaa gcattctggt ttctgacggt aaaacactgt ggttctataa
    63661 cccgttcgtt gagcaagcta cggcaacctg gctgaaagat gccaccggta atacgccgtt
    63721 tatgctgatt gcccgcaacc agtccagcga ctggcagcag tacaatatca aacagaatgg
    63781 cgatgacttt gttctgacgc cgaaagccag caatggcaat ctgaagcagt tcaccattaa
    63841 cgtgggacgt gatggcacaa tccatcagtt tagcgcggtg gagcaggacg atcagcgcag
    63901 cagttatcag ctgaaatccc agcaaaatgg ggctgtggat gcagcgaaat ttaccttcac
    63961 cccgccgcaa ggcgtcacgg tagatgatca acgtaagtag aggcacctga gtgagcaatc
    64021 tgtcgctcga tttttcggat aatacttttc aacccctggc cgcgcgtatg cggccagaaa
    64081 atttagcaca gtatatcggc cagcaacatt tgctggctgt ggggaagccg ttgccgcgcg
    64141 ctatcgaagc cgggcatctg cattctatga tcctctgggg gccgccgggt accggcaaaa
    64201 caaccctcgc tgaagtgatt gcccgctatg cgaacgctga tgtggaacgt atttctgccg
    64261 tcacctctgg cgtgaaagag attcgcgagg cgatcgaacg cgctcggcaa aaccgcaatg
    64321 caggtcgccg cactattctt tttgttgacg aagttcaccg tttcaacaaa agccagcagg
    64381 atgcatttct gccacatatt gaagacggca ccatcacttt tattggcgca accactgaaa
    64441 acccgtcgtt tgagcttaat tcggcactgc tttcccgtgc ccgtgtctat ctgttgaaat
    64501 ccctgagtac agaggatatt gagcaagtac taactcaggc gatggaagac aaaacccgtg
    64561 gctatggtgg tcaggatatt gttctgccag atgaaacacg acgcgccatt gctgaactgg
    64621 tgaatggcga cgcgcgccgg gcgttaaata cgctggaaat gatggcggat atggccgaag
    64681 tcgatgatag cggtaagcgg gtcctgaagc ctgaattact gaccgaaatc gccggtgaac
    64741 gtagcgcccg ctttgataac aaaggcgatc gcttttacga tctgatttcc gcattgcata
    64801 agtcggtacg tggtagcgca cccgatgcgg cgctctactg gtatgcgcga attattaccg
    64861 ccggtggcga tccgttatat gttgcgcgtc gttgcctggc aattgcttcc gaagatgtcg
    64921 gcaatgccga cccacgggcg atgcaggtgg caattgcggc ctgggattgc tttactcgcg
    64981 ttggcccggc ggaaggtgaa cgcgccattg ctcaggcgat tgtttacctg gcctgcgcgc
    65041 caaaaagcaa cgctgtctac actgcgttta aagccgcgct ggccgatgct cgcgaacgtc
    65101 cggattatga cgtgccggtt catttgcgta atgcgccgac gaaattaatg aaggaaatgg
    65161 gctacgggca ggaatatcgt tacgctcatg atgaagcaaa cgcttatgct gccggtgagg
    65221 tttacttccc gccggaaata gcacaaacac gctattattt cccgacaaac aggggccttg
    65281 aaggcaagat tggcgaaaag ctcgcctggc tggctgaaca ggatcaaaat agccccataa
    65341 aacgctaccg ttaatgttat cgttgcggta atgttgttaa tgtatccctg tggtcgcagg
    65401 ctgtggccac atctcccatt taattcgata agcacaggat aagcatgctc gatcccaatc
    65461 tgctgcgtaa tgagccagac gcagtcgctg aaaaactggc acgccggggc tttaagctgg
    65521 atgtagataa gctgggcgct cttgaggagc gtcgtaaagt attgcaggtc aaaacggaaa
    65581 acctgcaagc ggagcgtaac tcccgatcga aatccattgg ccaggcgaaa gcgcgcgggg
    65641 aagatatcga gcctttacgt ctggaagtga acaaactggg cgaagagctg gatgcagcaa
    65701 aagccgagct ggatgcttta caggctgaaa ttcgcgatat cgcgctaacc atccctaacc
    65761 tgcctgcaga tgaagtgccg gtaggtaaag acgaaaatga caacgttgaa gtcagtcgct
    65821 ggggcacccc gcgtgagttt gactttgaag ttcgtgacca tgtgacgctg ggtgaaatgc
    65881 actctggcct cgactttgca gccgcagtta agttgactgg ttcccgcttt gtggtaatga
    65941 aagggcagat tgctcgcatg caccgcgcac tgtcgcagtt tatgctggat ctgcataccg
    66001 aacagcatgg ctacagtgag aactatgttc cgtacctggt taaccaggac acgctgtacg
    66061 gtacgggtca gctgccgaaa tttgctggcg atctgttcca tactcgtccg ctggaagaag
    66121 aagcagacac cagtaactat gcgctgatcc caacggcaga agttccgctg accaacctgg
    66181 tacgcggtga aatcatcgat gaagatgatc tgccaattaa gatgaccgcc cacaccccat
    66241 gtttccgttc tgaagctggt tcatatggtc gtgacactcg tggtctgatc cgtatgcacc
    66301 agttcgacaa agttgaaatg gtgcagatcg tgcgcccgga agactcaatg gcggcgctgg
    66361 aagagatgac cggtcatgcg gaaaaagtcc tgcagctgct gggcctgccg taccgtaaaa
    66421 tcatcctttg taccggcgac atgggctttg gcgcttgcaa aacttatgac ctggaagtat
    66481 ggatcccggc acagaacacc taccgcgaga tctcttcctg ctccaacgtt tgggatttcc
    66541 aggcacgtcg tatgcaggca cgttgccgca gcaagtcgga caagaaaacc cgtctggttc
    66601 ataccctgaa cggttctggt cttgctgttg gtcgtacgct tgttgcagta atggaaaact
    66661 atcagcaggc tgatggtcgt attgaagtac cagaagttct gcgtccgtat atgaatggac
    66721 tggaatatat tggctaatac ccaatttttc tgaatctaaa aagcgcctgc gggcgcttta
    66781 agtagaggtt taatcatttg aaaataaatg gattttattt ttaagtgtcc atataacgtc
    66841 catctttgaa tgcaaaaagc ccacagcttc tatgctgtgg gctttgtttt ttggtgtcct
    66901 catgagtgag ggaaccaaaa agaaaacccg cagtttttac gctgcgggct tgttgttcac
    66961 gtcaactgga aatagggtgc cttttcgacc tttaccctgg caaccgattg acggggaagt
    67021 tgccttcccc gtcgcggttt ccttactgct tacactgtaa gaacgccgca aactccgctc
    67081 cccagaaact catccgtatt tcacacagcg aaccgtgcag catccagatg atgaggattg
    67141 ccgtcacgca gaatgtgatg gccgtaagcg atttttgcga catagcgctt gctccttttt
    67201 cggggaggcg ctaacctatc acttgctgag ggtagatggt tagggcctcg ggttaacgtt
    67261 aaagttgact cggggccttt ccacatctgg ccttcgggta ttccctccga ccatcagccg
    67321 aaaggcaccc gcacgtaatc tatcgctttt ttgttactcc ggcaattctg cctgttaatt
    67381 ctgagataaa ggcaaactca tctgattgtt tcccctgtgt gaagctggca gatcatgcca
    67441 cgggatacct tctgaagagt gaacgccgga ggcgtgtttt gatgtgaatt tatggaaagc
    67501 ttccagtgtt gagaagcata cgccgcattc cagattgtta cactggtaat acttttgccg
    67561 cacggtgttt gaatcatttt ccggacgact ggtgcggata cgggcagatg cgccacaaag
    67621 cggacaacgg aacatagcga cctcccttaa cgtggtgctg cctattctaa gttgctcact
    67681 ctgtttccgc tatccattcc gggatttttg cctcaagctc aagctgcgtg gtaaaaccgc
    67741 tgttatcaat ggtgtgctcg gcttttgcaa tgatccagtc ctgattatca atctcgcttt
    67801 taaatcctgt taccgtgcca tgcatttcgg ggtagagttc tgcacgtcca cgcgccagcg
    67861 tgatggagaa tgatgcggct ccgcgttgta gttgctgcca ctttgccgcc gctgcgcgtc
    67921 ttgctgcctg ctcgttctga taagtcttgc gtaacacaaa cacattgcct tccgcgcctt
    67981 ccatataatc accttcgcgg ctgctgcttt tctccttttt gggttttggc ggtttacggc
    68041 gtttcacgct gacttttttc tttttcccgt aattaagatc aagccagtaa gcgcgtaccc
    68101 ccgtatacgc ttcgcggtca gcaatgcgga actgatggcg atcgccgctg ctgcgtgtga
    68161 tggcaaacga tggcaacggc tggccctgtg cgttcacgcc accgccgggc atgatgaata
    68221 acagattacc gctttttacc gtggtgattg cgcccagcat ttccgccatg cgcgtaagga
    68281 aggacatgtc gctttcttcg gtctggtcgg cgtggtcgat ttcgatatcc atcagcattt
    68341 cgctgatttg cggtttcaga ccataccgat gagcgatggc ggataccaca cgctcaacgg
    68401 tcacatcatg ccaggacacc tcacgtttaa cgttaaattc atcccgaaaa tctgcgcttc
    68461 tggctgaaac agtcagcctg tccggcggtc cttcgtgagc gatttcatca acaatgtaag
    68521 tgcctttttc tgtcagcggt tctcctttcc agccaatgag aaccgtcagg cgcgcgcccc
    68581 gtggcggtag ctgcaactga ccatccgcat catccagcgt gatggtgagc tggtccgcct
    68641 caaatccccg gttgtcggtc agtgacaggc tcatcaggcg ctctgccacg cctgacagcg
    68701 ttttaccctc cgcgagaata tcaaaatccg gcattttcac ggggtctgtg ccctgactga
    68761 gcaattgcat ggtggtgtcg gtcatctgct ccctccctgt gcggcatggt cgcatgtgcg
    68821 tgcggagggg gttactgctt tttgttgtcg ccgtggcggg agaacggcgc aggggtgaga
    68881 ttacgcgcgt ggtgggtgat gattgttgcc gaatcattta acggatacaa ggggctgaag
    68941 ctatgagtga aactcgtttt catggtgccc gtgtcacgga aagtaccgac ctggtaacag
    69001 cgattaatga tgttgattcc agcgttatcg gtatcgtggc aacggcggat gatgcggatg
    69061 cggagctgtt cccgctgaac aagcccacac tgctgacccg cgtcaatgac gtgctgggaa
    69121 aatgcgggac tacggggacg ctttatcgtg cgcttaaggc tatcgcagac caggtgagca
    69181 caaaagtgat cgtcgttcgc gtggctgaac acaaagaaga aggcggtagg acgcaggatc
    69241 aactggttat cggtggttct gaatctgacg gcagctatac ggggatgtat gcgctgcttg
    69301 ttgcagagca ggatgaaagc atcggatacc gtccgcgtat tctggccgcg ccggagctgg
    69361 acacggaggc ggtgacaaaa tccctgtgcg tgattgcggg taaactgcgc gcgtttgtgt
    69421 atgccacatg tcatggttgt aacacgatgg ctgaggcgat tacctaccgc cagaaattca
    69481 acgaacgtga ggtgatgctc ttatggcctg acttcatcgc ctacaacccg aaaagtggca
    69541 aaaacgaaac gttccccgcg cctgcttatg cgtgcggcct tcgtgcgtac attgaccatg
    69601 agcagggctg gcacaaatcg ctgtccaacg ttccggttaa aaatgtgctg gggatgtcga
    69661 ggcatgtgtt ctggtcgtta caggccgaag acagtgatgc caacagcctc aacaacaaag
    69721 aaatcacgac cattattcgt cgtaacgggt tccgcttctg gggcaaccgc acaccggaaa
    69781 cgaacgccta catctttgag gtgtataccc gaaccgcaca ggtgctggct gattcaattg
    69841 cggaagcgca gtttgaaacc atcgacagtc cactgacgcc tgcgaacgtg aaggatgtta
    69901 tcagtgccat cagggcaaaa ttggattcgc tggttactgc cgggaaactg attggcgcgg
    69961 agtgctggta tgacgtggtg gataacggca ccacggattt acgtcagggg cgtgtgcgta
    70021 ttcgctacaa atatacgccc gttcccccgc tggaagacat ggagctttac cagacgttta
    70081 ctgatgaatt ctttggtccc gcatttgcgg tgctgggagg tgcctgatgg ctgtaccaaa
    70141 acatcttcgc ttttttacgt tgtttgtgga tggtgaaaac gaagtgggta aagtgacatc
    70201 cgtcacgccg cccaaactga cgcgcaaaac cgacagctat cgtggtggtg gcatgatggg
    70261 agcagtaagt attgatctcg gtctggacga ctccgcgctt gatgcgagct ttgtcatggg
    70321 gggagctgtt cgtgcactgt tccttaagta tggcggcacg attgacggca cgctgctgcg
    70381 ttttgcgggt gaatattaca ccgatgcaga aagcgatctg tatgaaatcg agatgcgcgg
    70441 gcgtgtgacg gaaattgata tgggggaggc caaacagggc gaagccacat cacacactta
    70501 cgctgtcaaa aacacctact acaagctgag cgttaacgat cgcccgctgt gggaaatcga
    70561 cctgttgaac cacatctacc ggaaggacgg caaggacatt gtgcctgacc gcattcgttc
    70621 cgcgcttggg cttggctgat aagtaatatg caggcggcgc agtgcgtcgc ctctgactga
    70681 aaaggagaca actgatgaaa gatatcgata ctgaaacccg gaataacacc gtagcggatg
    70741 atgtgacgtc aggtgaggat atggctgtcg aacgtggtgt aaaactttcc cggccaattg
    70801 agcgtggtgc cgaaaaaatc acatatgtgg agatcaccgg ggctattgag caggcgggat
    70861 ccctgcgtgg tctgtcgctg tctgatgtgc tgaatctgaa agcggatacc atgttcacgc
    70921 tgttgcctcg cgtgacctcg ccacgactgg atgaagtgat gattaaaaaa atgtcgtcac
    70981 gcgattttat tcagttgtgc gctgtggctg taaattttat gagcgagcca gactctggcg
    71041 cgaagagcgt gcaggagacg gcagcgtaat caccctggtg tgctttgagc acatcgaaga
    71101 tctggtggcg gatattgccg ccattttcaa ctggtcgccc gccgaaatct tcatgatgac
    71161 gcccggcgaa gtggttagct ggcgtgagcg ggcggcactt cgcagcggga atgcagataa
    71221 tgaagactct tgacatccgg gtcgctttca gcgccgttga caggctgacc cggcctgccg
    71281 aaaacgcccg ccgcctgatg gggcagtttg gtgactccat tcagcgaacg cagggggcga
    71341 tcaaaaatct cgagcgtcag gcgcgttcat ttgagcgcgc ccgcgacgct gtcagtaaag
    71401 cggatgcggg tatcgtgaaa gcacgacgcc agcttaacgc ccttaatcag ttacaacgca
    71461 cgggtacagt gctcagcgaa aaacaacaaa agctgatgca gcagttaagc acccggcttg
    71521 aacgcctgaa tgaatcgcgc acacgggaaa ttcagaaaat gcgggagctt ggcggagaac
    71581 tgaaacgcca cggcatttcc ctgacaggca gcgataacac catccagcag gccatcagac
    71641 gcaccgaaca atacaacaac cagcttgaac gcgaacggca ggcgcttgcg cgtgtaacgc
    71701 aggctcgtga gcggtattcg cgcgcgcagg aaaccgcggg aaaactgaaa acaggtggtg
    71761 cgctggcaat tggtgcggca gcggcgggcg gctatgctgc cgggcgtttt ttgcagcccg
    71821 cgatcgggtt cggcaaagag atgtcccgcg ttcaggcgct gacgcgaatc gacaaaaaca
    71881 gcccgcagtt taaggcgctg cgtgagcagg cgttaaaact tggctctgaa acacagttta
    71941 ctgcgagtga tgccgccagt gggcagagct ttctggcaat ggctggtttt actccgcagg
    72001 ccattcaggc cgcattgccc ggtgttctta atatggcgct ggcaggtggc gtcgaactcg
    72061 gcgagacggc tgatataggc tccaatatcc tcacacagtt caacctgaca gccgatcaaa
    72121 tggaccgggt tggcgatacg ctgacagcgg cattcacccg gaccaatact gatttacgcg
    72181 cgctgggcga aaccatgaag tataccggtc cggttgccgc aaaacttggt atcagtcttg
    72241 aagaagcggc agccatggcc gggatgcttg ccaataatgg tcttcgtgga agcgatgctg
    72301 gtacggccat gcgcgcaagt ctgtcccgcc ttgcatcacc gccaaaagct gcggctgatg
    72361 cgctgaaaga gctgggggtg tcagttgctg acgccagagg caaaatgcgc ccgatggagg
    72421 atgtgctgct tgatctctat aaggcgacac aaaaatacgg acaggtggac caggtttcct
    72481 tcttcaagga catcgccgga gaagaggcgt tcgttggttt gcagacgctt gttgcggcgg
    72541 ctggttcagg agagctgcaa aaactgacca gagaattgca gggggcaagg ggagaggccg
    72601 atcgcgttgc aaaagtaatg gccgataatc ttgatgggga cctgaaaaat ctcgacagcg
    72661 catgggaagg tcttcgtatt cgcatcagtg atctggttga cggtccgctg cgttctgtca
    72721 cgcagtggct cacgcgggtg cttgaaaaaa tcacctcgct ggcgcaggcc catccggtac
    72781 tgacgcgcca gctactgata gcaggcggtg cgttgctggc aatgactgca acggttggct
    72841 cgttgtcgct ggctattggt gtgcttgctg gcccgctggc aaaactgcgt cttggctttt
    72901 ccctcctgac cggttcaatg aatgctgtca gactcctgcc agcactatgg ggaatggtga
    72961 cgggttccgt ttcgttactg gggggcgcta tcggggcgtt gttcagtccg gtcggcctta
    73021 tcgtggctgc gcttgccgga gctgccgttc tcatctggaa atactgggat cccatcaggg
    73081 cattttttgc cggggtgttc agcgggatta tggaaaggct gaccccgttg cgcgaaacct
    73141 ttgaacggtt tggtcctgtt tttgacgcaa tcgggagtgg gatcagccag gtgtttaact
    73201 ggtttaaatc gctgctgtct ccgatggagt ccagcaagga aactctggat aaatgtacca
    73261 gtgctggcga gatattcggt aacgttcttg gcggtgcgtt acaacttgtt ctgacacctg
    73321 caaaaatgtt gctggatacg ctggcgtgga tacttgaaaa gcttggtgtg cttccggatg
    73381 aagcggaaag ggcgcgcaag aaaatcgaag acgcacagcg tgcggccatt cttcaggaca
    73441 aggttgcctt gcttcagggg gacctggcga aaatcaatcc gccgaagcct gtggaaaatg
    73501 gcaatggcac cggaggtgat aaccccaaag acaataaacc gctcacagac agcaataccg
    73561 gtacgctgcg cagactcagc aaaattgctg ataacacagg taagctggtt gaggagacga
    73621 aaaaacgcat tggccccggc gatattgtct ttaaaaacct gccccgagca cttgctgttc
    73681 gtggggagtg gcaggagcgg aagattgcgc aggtcagtaa gcctgccccc gcaattaata
    73741 tcacacccgt ggtcccggct ccgctgcctc cggcgctggt ccctgttgtt gcggccagct
    73801 cccgcccggt ggcggaggct atacgatctc cagtggcatc agttcctgta acttcccgta
    73861 accgggagcc tgttgcctcc ggatttggtg gtgaaattca tgttcatctg cataacgttg
    73921 ttacgcagaa tccccgcgaa ctggcgaaac tggtcggtga aatggtcagg gcagaaatgg
    73981 aacggcgcgc ccgtgccggg cgtggcagtt tttacgataa agattgagga gtcatggcca
    74041 tgatgatgat ctacggcatg tttgtttttg agctgcgcac gctgccgcat cagcagttac
    74101 agcaaaacaa aagctggcgg catgtgaaaa atgaacgcgt taaccgttca gcaagctggc
    74161 agtatatcgg tgcaggtgat gatcgcatcg ttctttctgg tgtgctttat cctgaaatta
    74221 caggtggcga agtgtcgctg tcgctgctga ccacgcaggc gtatacagga cgaccctggc
    74281 ctttgattga tggcgtcggg cagatttacg gcatgtatgt cctgactgaa acgaatacga
    74341 cccgctccga gtttgatcgc tacggtaagg cgaaaaagat agaattttca ctgactcttg
    74401 aacgctgtga tgaggatttg cgggagcgcc tgcaatcctc atcgttcagt gatatgctgt
    74461 ccggcttcaa agataaggtg acatcatctc ttaacagcgc ggccagttca gttaaagggc
    74521 tgttctgatt taacacaaaa accgctaatg gtcagattag cggttttcat tttcctgagt
    74581 ctgcccggtt gtttcttcag tctgtatatc gcctacaggg tgataacgat aaatcgtcga
    74641 tgtgccaatg ccgtaaatta ttgccagttg ttttctgtcg tgcccgtttt tgattagcct
    74701 tgctatttgc tcatgctgtt cttttgtcag cttcggtcga cgtccgcctg tgcgccccct
    74761 tgcgcgcgct gccgccagcc cggccagtgt acgttcaaca attaattcac gttccatttc
    74821 agccagggca cccatcacgt ggaagaaaaa acgccccatt ggagaagatg tgtctatgct
    74881 gtcggtcaga ctgcgaaaat taatccctcg ctcccgtagt tccccgacga gagaaatcag
    74941 atgtttcatg cttcgcccga ggcgatccag tttccagaca accagcgtgt cacctttttg
    75001 aaggcgcttt aaagcgcgtt ttaatcccgg tcggtctgtc tttgtcccgc ttaatttatc
    75061 ttcaaatatt tgttcacatc ctgcacaaac aagagcgttt cgttgcaggt ctgtattctg
    75121 gtcatttgtt gataccctta catagccaat cagcacactg aatctcccgt ccaaaagcgc
    75181 aaatcatgcc atgcaggcca gaaacggcca ttatttaa
//
LOCUS       DACADF010000004        69234 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.004, whole genome shotgun sequence.
ACCESSION   DACADF010000004 DACADF010000000
VERSION     DACADF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.004"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            179..1267
                     /gene="ompF"
                     /locus_tag="ISB30_000250"
     CDS             179..1267
                     /gene="ompF"
                     /locus_tag="ISB30_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAP1987935.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGEDSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESPLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVDGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            1452..2642
                     /locus_tag="ISB30_000251"
     CDS             1452..2642
                     /locus_tag="ISB30_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAP1987936.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(2692..3339)
                     /gene="gloC"
                     /locus_tag="ISB30_000252"
     CDS             complement(2692..3339)
                     /gene="gloC"
                     /locus_tag="ISB30_000252"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAP1987937.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFNEQARLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3366..3914)
                     /locus_tag="ISB30_000253"
     CDS             complement(3366..3914)
                     /locus_tag="ISB30_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAP1987938.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(4095..5942)
                     /gene="ldtD"
                     /locus_tag="ISB30_000254"
     CDS             complement(4095..5942)
                     /gene="ldtD"
                     /locus_tag="ISB30_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAP1987939.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKLMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(6203..10663)
                     /gene="mukB"
                     /locus_tag="ISB30_000255"
     CDS             complement(6203..10663)
                     /gene="mukB"
                     /locus_tag="ISB30_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAP1987940.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVWKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(10663..11367)
                     /gene="mukE"
                     /locus_tag="ISB30_000256"
     CDS             complement(10663..11367)
                     /gene="mukE"
                     /locus_tag="ISB30_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAP1987941.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(11348..12670)
                     /gene="mukF"
                     /locus_tag="ISB30_000257"
     CDS             complement(11348..12670)
                     /gene="mukF"
                     /locus_tag="ISB30_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAP1987942.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(12667..13452)
                     /gene="cmoM"
                     /locus_tag="ISB30_000258"
     CDS             complement(12667..13452)
                     /gene="cmoM"
                     /locus_tag="ISB30_000258"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAP1987943.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEIGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQILGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            13588..14367
                     /gene="elyC"
                     /locus_tag="ISB30_000259"
     CDS             13588..14367
                     /gene="elyC"
                     /locus_tag="ISB30_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAP1987944.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            complement(14344..15237)
                     /locus_tag="ISB30_000260"
     CDS             complement(14344..15237)
                     /locus_tag="ISB30_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAP1987945.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLSMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFNRRNFDLASKSLLPWLA"
     gene            complement(15391..16137)
                     /gene="kdsB"
                     /locus_tag="ISB30_000261"
     CDS             complement(15391..16137)
                     /gene="kdsB"
                     /locus_tag="ISB30_000261"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAP1987946.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(16134..16316)
                     /locus_tag="ISB30_000262"
     CDS             complement(16134..16316)
                     /locus_tag="ISB30_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAP1987947.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADEGKS"
     gene            complement(16368..17600)
                     /locus_tag="ISB30_000263"
     CDS             complement(16368..17600)
                     /locus_tag="ISB30_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAP1987948.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPHRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIALLIQHIHDRGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDSKMHRQKGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     SQGLFTDCRAGWEIDPVA"
     gene            complement(17637..18623)
                     /gene="lpxK"
                     /locus_tag="ISB30_000264"
     CDS             complement(17637..18623)
                     /gene="lpxK"
                     /locus_tag="ISB30_000264"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAP1987949.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKLVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAIAGIGHPPRFFATLKMCGVQPEKCVPLADHQFLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            complement(18620..20368)
                     /gene="msbA"
                     /locus_tag="ISB30_000265"
     CDS             complement(18620..20368)
                     /gene="msbA"
                     /locus_tag="ISB30_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAP1987950.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(20405..22669)
                     /locus_tag="ISB30_000266"
     CDS             complement(20405..22669)
                     /locus_tag="ISB30_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HAP1987951.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWLHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKYRYHQQGYKWLDTPHQGQITVNFSAQGWWISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(22875..23159)
                     /gene="ihfB"
                     /locus_tag="ISB30_000267"
     CDS             complement(22875..23159)
                     /gene="ihfB"
                     /locus_tag="ISB30_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAP1987952.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(23319..24992)
                     /gene="rpsA"
                     /locus_tag="ISB30_000268"
     CDS             complement(23319..24992)
                     /gene="rpsA"
                     /locus_tag="ISB30_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP1987953.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(25103..25786)
                     /gene="cmk"
                     /locus_tag="ISB30_000269"
     CDS             complement(25103..25786)
                     /gene="cmk"
                     /locus_tag="ISB30_000269"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP1987954.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(25959..26741)
                     /locus_tag="ISB30_000270"
     CDS             complement(25959..26741)
                     /locus_tag="ISB30_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAP1987955.1"
                     /translation="MYLKSNQKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNA
                     YKAATLSDADAKAIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYK
                     VYMTSDVNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAI
                     VAARDAISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQ
                     GLVGSFEKLASLDGGHTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(26885..27274)
                     /locus_tag="ISB30_000271"
     CDS             complement(26885..27274)
                     /locus_tag="ISB30_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP1987956.1"
                     /translation="MSDLNQFRSKGPELLVELAQHTSETVREIIDIEPEVADQIGQAV
                     ANRMMQVWGGQNVYFPMGMVWKVSQRDREIFREFNGRNHHELARKFGVSLQWVYSVVK
                     RVRKEELDRMQGKLFADEPDADTEKKE"
     gene            complement(27246..27695)
                     /locus_tag="ISB30_000272"
     CDS             complement(27246..27695)
                     /locus_tag="ISB30_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HAP1987957.1"
                     /translation="MQKKRLIQLIHIARNELGMDEDTYRQMLQGLTGKASTKGMDTTQ
                     LNCVLESMKKKGFRVKPARKASSGLPLDNHPQSRKIRALWLEMAAAGIVRDRSENALA
                     RWIKRETGISALRWLSTEQASSVIEKLKKWQRRAAGVKHERPESVSK"
     gene            complement(27697..27903)
                     /locus_tag="ISB30_000273"
     CDS             complement(27697..27903)
                     /locus_tag="ISB30_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987958.1"
                     /translation="MTTETIVCFLFWYMYAGSCSARLHRSLGYGRHYDTAHYILYMTA
                     VMLFWPVTLPAATDIVADRLKKRR"
     gene            complement(27893..28123)
                     /locus_tag="ISB30_000274"
     CDS             complement(27893..28123)
                     /locus_tag="ISB30_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987959.1"
                     /translation="MITLSGKSRKLKACRISARYLFARAFFKNVRPGITIGVIAGREQ
                     VEKYMSGAWWNNDPVIAARNIHIEWGGIQNDY"
     gene            complement(28120..28803)
                     /locus_tag="ISB30_000275"
     CDS             complement(28120..28803)
                     /locus_tag="ISB30_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HAP1987960.1"
                     /translation="MTDQNKHIEKLKKLLALAASGNPHEAALALRRARKLMDVHGITH
                     SDIAMSDIDETISHYWPTGSLRPPRYMLGLMNIIREVFGVNSIIHPGTHPSVGFYGNR
                     ERAALAAYTWEVLARQLKKARQQYISAQNKRIKTATRTSRGDQFAEGWVLAVISEIQS
                     FALTDDERELMQQWLEHKYPQTQTTRARKPGRSRNGDASRYAGFREGQNVRLHRPVSG
                     QEQQKLEAR"
     gene            complement(28800..29015)
                     /locus_tag="ISB30_000276"
     CDS             complement(28800..29015)
                     /locus_tag="ISB30_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987961.1"
                     /translation="MADILREITAWTLILTGLATCLSAGAALAALLMHITTQWLWEKL
                     KAAYSLKELSDAVRAWKRQKNTGDTEQ"
     gene            complement(29030..29326)
                     /locus_tag="ISB30_000277"
     CDS             complement(29030..29326)
                     /locus_tag="ISB30_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987962.1"
                     /translation="MTRNTELTRTALYRLALQRFGPDAQALKLAEEAAELAASAARNL
                     NGQGSESDLAAELADVEIMTEQLRLQGMDRLIDFHKQKKLERLAARLGVIYTNE"
     gene            complement(29336..29608)
                     /locus_tag="ISB30_000278"
     CDS             complement(29336..29608)
                     /locus_tag="ISB30_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987963.1"
                     /translation="MITPQEARQRTRTLVEHYVNECECRDLTDVKHVLTALISMATQA
                     IVATNGKEAALQVLMNTLTHTAEHEVPYRMETTAEGGLHITVSRKH"
     gene            complement(29897..30427)
                     /locus_tag="ISB30_000279"
     CDS             complement(29897..30427)
                     /locus_tag="ISB30_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAP1987964.1"
                     /translation="MAKSTKGAKRIKAAAALWVPGTREEVIEGIRLLGDAQRELVRAE
                     TEMNDAIGDITARYAPLTESLKKRMAELQSGIQTWCEAHRDELTGNGKVKFANLTTGE
                     VQWRNRPPSVSIRGADNVIELLRRLGLERFIRVKEEINKDAILNEKEAVKNIPGISIK
                     SDIEDFSIIPFEQDVQ"
     gene            complement(30455..30724)
                     /locus_tag="ISB30_000280"
     CDS             complement(30455..30724)
                     /locus_tag="ISB30_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987965.1"
                     /translation="MKSTTGINQQISKVQSAIMALKATNTDVQSITIRGNKPVIRVSR
                     SAHCMRMLEQGKACYLYTGHDHRGYFRQGVFELHGCRVVWPESLW"
     gene            complement(30727..31893)
                     /locus_tag="ISB30_000281"
     CDS             complement(30727..31893)
                     /locus_tag="ISB30_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP1987966.1"
                     /translation="MLVLKQQLKEARIPQAVVARAVDVSEATLAQIVNHNAWPRTSPG
                     EVRRRLASWLESQGIDTTKSFDAVQGAATPRTAGTTDKTSLSEEENMLLKKQVLFPAT
                     KKAFGLFRDPFADEAMQGSDDVFTTPDIRYVREALYQTARHGGFMAVIGESGAGKSTL
                     RRDLTERINRENAPVIVIEPYIIAMEDNDVKGKTLKAAAIAEAIISTIAPLESIRRSQ
                     DARFRQLHRVLKDSSQAGFSHVLVIEEAHSLPIPTLKHLKRFFELESGFKKLLSIVLI
                     GQPELATKLSERNMEVREVVQRCEVVELLPLDNNLEEFLTFKLQRAGKQLTDIMDAGA
                     VDAIRARLSNPGSHRKNMVSLLYPLAVSNLVIAAMNLAAEIGVPQVNADVVKGV"
     gene            complement(31904..33673)
                     /locus_tag="ISB30_000282"
     CDS             complement(31904..33673)
                     /locus_tag="ISB30_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1987967.1"
                     /translation="MTPALTEKLVETARAARDAGHGKRGAIYDAACAELGMSRATLLR
                     RLKEVSVTDKRKKRADAGRSALTRDEAALISATLREATRKNGKRLYSIADAVETLRAN
                     GFITAGRTDETTSEFFPLSEDAISRALRNYGLHPEQLDAPAPHTEVASLHPNHVWQID
                     ASLCTLYYLSNGHKGLQVMDSAKFYKNKPANLARIASDRVWSYEITDHASGWIYVEYV
                     TGAESGENLCSVLINAMQERGGADVLHGVPKILYLDPGSANTAGMTKNMCRSLGIDLI
                     AHKPHNARATGQVEKARDIIERKLEPGLKFRPVHSLEELNALAAKWRSHFNATAVHSR
                     HGKTRTDIWLKITAEQLKKAPSVEVCRELAVAAPELRKVTPKLRVSFRGTEFDVSTVP
                     GVLVGEKLMITRNPWRSDVAQVVLTGEDGHETFFLVEEVRKNEFGFAEGAAVFGESYK
                     ALPETPAQMAAKETEALVTGTDNAADAAAARKAKALPFGGRLDPYKHIDDTTLPAYMP
                     KRGQASDVRGPRTEQRPMTHVEAAKALRDKFSADGLTWTPEHYRQLTAQYPDGVPEAA
                     LDEVMATLTTPARSSVISIVNGN"
     gene            complement(33689..34006)
                     /locus_tag="ISB30_000283"
     CDS             complement(33689..34006)
                     /locus_tag="ISB30_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987968.1"
                     /translation="MNTNNTQNTLMDSPEALGHALCNLLPEMVQGFRVVTPSGEICVP
                     AQETHPFVLTMEVMLMQQIRRLQNQSALRPVVAPQPVNTVVKTCDGETLCDLARKIAA
                     RIG"
     gene            complement(34006..34926)
                     /locus_tag="ISB30_000284"
     CDS             complement(34006..34926)
                     /locus_tag="ISB30_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HAP1987969.1"
                     /translation="MGRTKSPINTELNADVPLSDDLNVSLNAMTQHRMEIMQQFGDGL
                     PYERERIVHEARFYMAQSAESMLEAGKRLIILKENEPHGDFTNILENDLGLAPQVARR
                     MMQASVKFLGNGDEQPKRSALSVLGKTKLYELMVLDDEELDALADGGTVAGATLDDID
                     RMTSRELKAALREARETNAAQQQVLAGKDEKINELATKLEKKSRLQPPPPDEELKKLR
                     AEVTALAVEAESAIAVRLSSVFETLCEYCTKNMIDTPRDFMAGLVCQIERSVHALRET
                     FDLEAAPSGNDAPAWLTEPEPEINRPEDMQ"
     gene            complement(34937..35245)
                     /locus_tag="ISB30_000285"
     CDS             complement(34937..35245)
                     /locus_tag="ISB30_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1987970.1"
                     /translation="MSKTNVSSSGSRILRVLKALRGHALNGISNGELATALHESPANI
                     NRALNTLIEEGLALKLENGRFAPGIQLLQIAMAHSNEMARAQDRINEINQRVISGSRL
                     "
     gene            complement(35298..35486)
                     /locus_tag="ISB30_000286"
     CDS             complement(35298..35486)
                     /locus_tag="ISB30_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP1987971.1"
                     /translation="MTAEQVKALFRQRGITFTRWAEENGYNRNEVYRVLNGQTKARYG
                     KSHEIAVKLGLKSTAQAA"
     gene            35580..35936
                     /locus_tag="ISB30_000287"
     CDS             35580..35936
                     /locus_tag="ISB30_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1987972.1"
                     /translation="MSKDIGERVISVRKKAGLNQREFAARLGISNGGISQIENGKAMP
                     GGEFLLKMHQEFNVDVNWLLTGMSTDGMTRNQNQISPEKQKLIDTFDDMTPDQQRSFL
                     EIGRFYTQPKPGKIAG"
     gene            complement(36053..36817)
                     /locus_tag="ISB30_000288"
     CDS             complement(36053..36817)
                     /locus_tag="ISB30_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HAP1987973.1"
                     /translation="MKMQTLPIVPWIGGKRRLAKHILPLFPAHECYVEPFCGAAALYF
                     LKTPGKIEVINDINGELVNLYRVVKHHLEEFVRQFKWALVSRQIYKWLQITPEETLTD
                     IQRAARFYYLQKQAFGGKVAEHSFGTSTTSPPRFNLLRIEEELSAAHLRLSRTVIEHM
                     DWQQCIERYDRPHTLFYCDPPYLGTEGYGVDFPEGNYSRLAELARCIRGKMIISVNDI
                     PQMREVFTGLNIQTVNINYSLAGKPTPRRELLICNF"
     gene            complement(37008..37223)
                     /locus_tag="ISB30_000289"
     CDS             complement(37008..37223)
                     /locus_tag="ISB30_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987974.1"
                     /translation="MNPRLTLTEHQRRAEAVNNVLEDIIRLYRGELSVCRAAFHFQGI
                     QKQFDTSVFAEGITYALDRIRSENRPG"
     gene            37222..37626
                     /locus_tag="ISB30_000290"
     CDS             37222..37626
                     /locus_tag="ISB30_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987975.1"
                     /translation="MMHSLNDDFYVLTGKIFCRLLNTHPAPQVLIYSGFFDIPDQVDH
                     DQKKCRCFIDTLIWLRDNSYIRYSQLLAVGAADVVLTEKTLCLLNAMPTFLQPRPVPF
                     KESLLDAVKTGENAVIAECVSRIMRYVFNERK"
     gene            complement(37602..38330)
                     /locus_tag="ISB30_000291"
     CDS             complement(37602..38330)
                     /locus_tag="ISB30_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987976.1"
                     /translation="MTQTLNPVITVTVSGPVGSGKSYVLARIEEVVKQEFGNSVIVDA
                     ADVDGERRMSGDDLSTWQKPRAGTVIRLEEYTGQGLLSYGEERICRTNELLDVICPTG
                     RQPWILPDALHATIAALMTVYDQELLMKELRKLTGQSTECCSSPKYGKQEHIPINQPP
                     RFFDEYLLTPENKAEHEEIKAALFREIRKIAEMQLEGGLDRVMARGEWASLFERVSPE
                     LLPLALAIGIMQTADKPHLRSLNT"
     gene            38461..38811
                     /locus_tag="ISB30_000292"
     CDS             38461..38811
                     /locus_tag="ISB30_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006118559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987977.1"
                     /translation="MKNLKKFIPPVKKPRLSGWLLTSVLLLGTIALVSPQQLPVVIYK
                     LALITLAAVLGYWLDRSLFPKARPGQYLKHDDRLMAEGRFPVQTGLHLVFSAALIRRA
                     LIVAAVCLAVAMGL"
     gene            38814..39554
                     /locus_tag="ISB30_000293"
     CDS             38814..39554
                     /locus_tag="ISB30_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP1987978.1"
                     /translation="MNWPQITWIVCMSLKLAFEAFRVFIRTERLSVRAGTFLVHMAWV
                     FFVAMLLWCGGFFSQACAAQPPQAALQYRDDVIRNARLEWGLSAPVADFAAQLHQESG
                     WRPDAISPAGAQGLAQFMPATADWISQLIPMLSSREPFNPAWAIRALVSYDRWLWQRV
                     SAASDCERMAMTLSGYNGGLGWVQRDRRLASQKGLDSTRWFGHVATVNAGRNAASWRE
                     NRHYPQRILRELAPRYLTWGGSSCVASG"
     gene            39538..40188
                     /locus_tag="ISB30_000294"
     CDS             39538..40188
                     /locus_tag="ISB30_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987979.1"
                     /translation="MWHLVKKLPWRGILLAILINAFLVGLYAMGYRSGHDSAKRDGDT
                     ALSQLQSAFDAYKTEQATLENAALRAWARRYQEQVAAGQRAEAGYLEQIAQLESRNKQ
                     LQGQINDVTQRWIDEKGKSHPIECVFTRGFVRQYNAALGYDNASVDTGHSDSVAATGT
                     RSGTATGQPETTDTRLRDSGVSQRDVLANIIDNAWQCRRWRNQINALLDEREGLQK"
     gene            40185..40511
                     /locus_tag="ISB30_000295"
     CDS             40185..40511
                     /locus_tag="ISB30_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987980.1"
                     /translation="MTLQVEFWTVVSFLLTFMRFVGGLAKWLFSKTEERQAARFASLE
                     QALQQSASNWGELEKEFMRFKADLPLNYVRREDYIRGQTVIEAKLDALYNKLEVVQQY
                     RNTGGQ"
     gene            40511..40822
                     /locus_tag="ISB30_000296"
     CDS             40511..40822
                     /locus_tag="ISB30_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987981.1"
                     /translation="MVDITRVRRESLRWSLLVALNKTRPYTASETLLLDVSRAIYPDT
                     TPLELRRELDYLADRKMVELEKKPSGDWFADLTRLGVDLVEYTVECGPGIARPEKYWS
                     E"
     gene            40822..41367
                     /locus_tag="ISB30_000297"
     CDS             40822..41367
                     /locus_tag="ISB30_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HAP1987982.1"
                     /translation="MARRSTIEKLPEDVRRWLERALTESGFSGYNELESLLRERGYVI
                     SKSAIHRYGQKIERRYGAIRAATEAARMLTEGAADDQDARSEAVIALIQTELFESIVQ
                     LQEAEEGEVDPKERVALLSKVAKNVATLSRASVNLKKFQSEVRARAQQAASNAEKIAR
                     KGGLSSDAVQALRREILGIAT"
     gene            41427..42959
                     /locus_tag="ISB30_000298"
     CDS             41427..42959
                     /locus_tag="ISB30_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987983.1"
                     /translation="MDGRNNLAPVLPDTSALDAPPVLLPYQQRWVADTSPLKVIEKSR
                     RTGITWAEASDNVLTAASSAPAGGMNVYYIAYNQDMTVEYIQACAMWARAFNYAASEI
                     EEGFWEEDDDDKHIKTYTIKFPDSGFRIVALSSRPSNLRGRQGIIVIDEAAFHEQLDE
                     LLKAALAMLIWGGKVRVISTHDGDDNPFNTLIGDIRAGRQGGSIHRITFREAVSEGLF
                     RRVCLRTGKEWSEASEQAWMASVYKFYGAGASEELDCIPANGGGAWLSRALIESRMSA
                     DTPVLRLTCKEGYELLSDEVRFRETQDWLDEYLKPLLEALPTDARSFLGRDFGRSGDL
                     SVDYPLLQEKNLVRRVPFVLELRNVPFRQQEQITWYLMDGLPGLLGAAFDARGNGAYL
                     AEYAMQRYGSGRVQQVMPTEGWYREHMPPVKAALEDGNLVDLPKDEDTLDDLRAVQVV
                     NGVPRVPEQRSKAKADGGKRHGDSAIALALAYFASREINKGPVKASSRRRRQAARMLE
                     GF"
     gene            42959..44455
                     /locus_tag="ISB30_000299"
     CDS             42959..44455
                     /locus_tag="ISB30_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HAP1987984.1"
                     /translation="MARGIWVSPDEFVAFSEPQKSLTAQIASRSRAIDFYGLGMYLPN
                     PDPILKAQGRDIRIYRELRTDPLVGGCIRRRKAALKSLERGLERGHASARVFRFIRDM
                     LDDLDLSRIIGEMSDAVLYGYQPCEIMWGRSVRSWAVTDIVGKPPEWFQFDTDNCLRF
                     RARDAGVEGELLSPSKFVVPAQDASYDNPYGFPDLSMCFWPVAFKKGGMKFWLRFAEK
                     FGSPWVIGKHPRGANDAEIEKLLDSMEQMVEDAVAAIPDDSSIELRAADGKADSSEVF
                     RELITLSRSEISIALLGQNQTTEANSNKASAQAGLEVTADIRDADADIIQAAVNQVIR
                     TVVTLNFGDVPCPVWAMWEQEAIDDTRATRDEKLTRAGLRLTPQYFKREYQLQDGDID
                     ETPPSERQNNMLPLSFAEAIDADIQAQQQLDDALDILMNGGALNGTLEPVLAPLFRRV
                     ENGVNPSELLGELAELYPQMNTDDLQERLARILFVANIRGRLHERDNG"
     gene            44436..45257
                     /locus_tag="ISB30_000300"
     CDS             44436..45257
                     /locus_tag="ISB30_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP1987985.1"
                     /translation="MSVTTAELAYCMTLPPKRAINYLKSKGYQITWDWEEMWQEAHAR
                     AFTVAKVTRLDILEDIRGALQQAVDEGKTDRWFRQELEPVLKRKGWWGPRDTTDPVTG
                     EPVTIQQGSPWRLDTIFRTNMSVLYSAGRWAEQMENVDDRPYWMYTGINDSHTRRSHL
                     ALHGLVLRWDDPFWQAFYPPNGWRCRCSVIALSAADVRARGLKVISSGSAMGQELKLV
                     SEKTGEMRNVATFNTGTTKVTTDVGWSYAPGAAYRPDLARYQGTLQPLAQQELRG"
     gene            45260..45718
                     /locus_tag="ISB30_000301"
     CDS             45260..45718
                     /locus_tag="ISB30_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HAP1987986.1"
                     /translation="MASDNLVSITINDKSLRRSLRALDLAATDLEPAMRKIAGTLLAE
                     TQFNFLDEGRPGWMPSLAAEERDGQTLQDTGRLMGSVSTDHDDRQAVVGTNVVYGAIH
                     QFGGKTGRNESVELPARPFLPVTGDGELQPEVVIPILDTIVRHLESAARR"
     gene            45933..47048
                     /locus_tag="ISB30_000302"
     CDS             45933..47048
                     /locus_tag="ISB30_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP1987987.1"
                     /translation="MSAIHIFKAGTHTDMHGKKLPFTPDDLAACVKAYDPSVHEAPLV
                     IGHPRTEDPAWGWVKALSLSGVDLMAEPAQLDPQFAEMVTDGRFKKVSASFYLPDSPS
                     NPKPGVLYLRHVGFLGAQPPSVKGLKQVSFSEQEEGVVEFADWQAITNASLWGKLRDF
                     LIARFSLDEAEKVLPEWQLNSLREEAYRDTLSQDAAGAQFSETGPGPSSASNEESSMT
                     KEEIEALQEENRRLKQQAADRDARDAQVRQEQLHKDNVAFAEKLVAEGRLAPRASSVV
                     VALLDAVAGGDKPVEFAEGESRTPLATAFRSLLSDGEPVMNFAEQATKERVGDTVKVD
                     VAEFAEADPERLALHQKAVALSKKEGISYEAAVARCL"
     gene            47063..48016
                     /locus_tag="ISB30_000303"
     CDS             47063..48016
                     /locus_tag="ISB30_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987988.1"
                     /translation="MSDYLKGKRVVDPVLTSIARGYKNAAFIGERIFPVVLTDKEGVR
                     VPTFGKTAFVEYDTERAVGADSNVLVREKTGTLDLVLGEHDLAAPVDYREQAESMFNE
                     ESKAIRRATNGVNLRRELIAARLAQDEKVYRTGHVKKLTASDRWAGGKGDPIGVIEAG
                     MEAVRTATGLRPNLMTMGAGVMALLKFHPAIQAAIGANERKRITTEILQDLFQIEEIV
                     IGAPVSLPSMKAAMDKNSVPADIWGDNLMLHYVGKPQPGADSADENEPSFGYTLRRKG
                     MPVADKYDGAGGKVKYCRYTDIYKVAVVGGDAGYLITGISK"
     gene            48026..48364
                     /locus_tag="ISB30_000304"
     CDS             48026..48364
                     /locus_tag="ISB30_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HAP1987989.1"
                     /translation="MGTTQQVILITTVTAGAALAQQRFVGADNTPCKAGAAALGVAEV
                     DAVTGDSTPVSVLGIIAVEAGAAVSRGVAVQSDAQARAVPQSGDGKSCGIALDEAGGE
                     GDVIRILRGV"
     gene            48366..48812
                     /locus_tag="ISB30_000305"
     CDS             48366..48812
                     /locus_tag="ISB30_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HAP1987990.1"
                     /translation="MYCTLEDLLAQVPERTLIELTSEEMDFDSPATVNTRVVDSCIRY
                     ADELIDAHLRGRYILPLAEIPTVLRDIAITLVRYRLYARRPEGDLPDTVKDDHKEALR
                     QLRELRDNRLTLGLPSTQKDVPEPGEFRVRSRPATFGGRDGLLEKY"
     gene            48812..49276
                     /locus_tag="ISB30_000306"
     CDS             48812..49276
                     /locus_tag="ISB30_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987991.1"
                     /translation="MNVLPVLDAVLARLREKLPQLQVEYFPEKPAEYRLNHPVGALLL
                     SYAGSRFDRPDDTGAVIQSQTIQLCVTVVFRQLNGKKGAINVLDAVRRILGGHTPPGC
                     RRRIWLTREVFIGEVRGLWQYALDFATESVFIEDSDLPSGPLLTEVNYEESE"
     gene            49273..49527
                     /locus_tag="ISB30_000307"
     CDS             49273..49527
                     /locus_tag="ISB30_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987992.1"
                     /translation="MMKEYRYSGPASGVTLSDGTEILLWPGKTVSLPEEHDYVKVLVA
                     LKHLTPVPEETKPAGTPAVQSPKRRSSSDSEVKTEDTHGS"
     gene            49517..50944
                     /locus_tag="ISB30_000308"
     CDS             49517..50944
                     /locus_tag="ISB30_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HAP1987993.1"
                     /translation="MAANYLHGPETIEVENGARPVKTVKSAVIGLIGTAPMGDVNTLV
                     QCLSEKDAAAFGSQFTGFTIPQALDAIYDHGAGTVLVINVLDPAKHKTAIEDEVVTFD
                     KSTGQARLAHPVVANVVVKNSEGSTTHTANTDYRVDAQAGVLTNLGKAIEAGGSVKVS
                     YEYADPSKVTAADIIGGVNSAGNRTGMKLLNDSFNLYGYFAKILIAPVFCTQKSVAVE
                     LIAMAEKLGAVTYIDAPVGTTFAQALAGRGPEGTINFNTSSDRVRLCYPHVKVYDPVT
                     NTERLEPLSQRAAGLRARVDLDKGYWWSSSNQEILGITGVERQLSAMIDDPQSEVNLL
                     NEQGITTVFSSYGSGLRLWGNRTAAWPTVTHMRNFENVRRTGDVINESIRYFSQQYID
                     MPITQALIDALTESVNAYGRKMTGDGAVLGFRCWFDPARNPETELAAGHLLLSYKYTP
                     PPPLERLTFETEITSEYLLTLKGGN"
     gene            50944..51465
                     /locus_tag="ISB30_000309"
     CDS             50944..51465
                     /locus_tag="ISB30_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HAP1987994.1"
                     /translation="MSKIEINRITNANIYLDGTNLLGRAEEVKLPDVSMIMQEHKALG
                     MVGKVELPAGFDKLEGEIKWNSFYRDAMLSAANPYRSLALQCRSSVQRYSSQGLIDEI
                     PLVTFLTIMFKKNPLGTFKQHENAEFSSSFTCTYIRQVLDGEELLQLDYLANIFRVGG
                     VDQLTDYRINIGG"
     gene            51468..51749
                     /locus_tag="ISB30_000310"
     CDS             51468..51749
                     /locus_tag="ISB30_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1987995.1"
                     /translation="MSVELTDKGGRCAALGMSNGTWFTLLDIPGVETLFNTRKTNDPI
                     DCTRSKARKLADLIEAWEPPDHWFSGIGKSEGKTLLIAFLRNCKGFRTC"
     gene            51847..52182
                     /locus_tag="ISB30_000311"
     CDS             51847..52182
                     /locus_tag="ISB30_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAP1987996.1"
                     /translation="MSQTPSDTFKLSYPFTTAAGTRIELVELKRLTVKDLKQVRKISK
                     NPADWDEPLIARSTGLLPEDLDNMDLADYLQLQKRFQLITGMGESNQGADAGAGAAGE
                     MVPVSAGGD"
     gene            52106..52264
                     /locus_tag="ISB30_000312"
     CDS             52106..52264
                     /locus_tag="ISB30_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HAP1987997.1"
                     /translation="MRATRALTQAQGLLARWFRFQPGEIDALDTDDLEMWLEQAEEQI
                     KSEYGDKS"
     gene            52340..55291
                     /locus_tag="ISB30_000313"
     CDS             52340..55291
                     /locus_tag="ISB30_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP1987998.1"
                     /translation="MSGQFSVGVVIGGMIGSTFRSAMSGTRRALDSLSDTSRRLQERQ
                     NALTRATERYGQLGSSRMQHLNSELLRVSRTMEQIERQQRRLSAASATSDALKANRMA
                     LYGQGIEAYGMAQTVYHTVSPAVQQSMSFQDKMIDMSITAKYDNKTRDALAGQIKGWA
                     LKYNQYQDELQEAMGSLISDNIDNVSDIGFLMPDIARAATATRTSAQDWAKVAAVWQN
                     SLKGAARDFGAVQNIMAYAGDQGSFEIPDQVKWMQSLAPMMAGIASGKEAVAEIGASL
                     QIAKIGAGSTDEAANNFKNFLTKIFARDTQKQFADLGIDLQGSIASYKAAGISPIEGM
                     LSVIERYLNAKSPEALAGFKSAMKIKNDTARDEALQALAKNFGLGDMFADMQVMAFIR
                     PMLANMDRYREIRAGALRAADNDLLASAYDQRLKSPLEATKTLMVSSRDLAITLGDQL
                     APSFISLTQDLLPLIQGTKHWVATHPQFVSGAFKLISALLAIKIATVGLKLGLNLLIS
                     PFVNVWKTAVLLRTNWHRLTTALGEGGKLRWLVTGFSRLTSGGLKLSKVLAGSLVRGF
                     MSAARAVLWIGRALMMNPIGLVITAVAAAAYLIYRNWGAVSGWFKQRWADIQEAFNGG
                     IAGTGKLLINWSPAGLLYKAFAAALKYFGVTLPAKFTDFGGHLIDGLINGIKNKWGSL
                     KSSVTGMGDSISSWFKQCWADIQEAFNGGIAGTGKLLINWSPAGLLYKAFAAALKYLG
                     VDLPAKFTDFGGHLVDGLINGIKNKWESLKSSVTGMGDSISGWFSEKLGIHSPSRVFM
                     GFGDNIAQGAAIGLQRTTPLAALAGQRMASELLPRMPVSIQGPEIRDNTSGVRFSMPL
                     PDINGFMSSAKNAIGAVINSLSSMPAIPLSTRASILPGQRLANEMTPDVPRIPSPEIL
                     AAGYSGRGAAATGGGTSGGIQVSFNPQFFLNGRETTAPAGLTGALNMSLHELEKMLER
                     LLAQKQRKEYR"
     gene            55291..56175
                     /locus_tag="ISB30_000314"
     CDS             55291..56175
                     /locus_tag="ISB30_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1987999.1"
                     /translation="MFAVLGDIEFELITYWDGFEATFGVDYAEHARIGGKPGLQFVGD
                     RLDEIQITLVFHQHYCVPDVELARLRTAMKAHQALALVFGNGDYRGWFVITDVTATSE
                     QTDSTGNVLAVSATVSLREYTGDPKNPLQPPAIRTKLPGVGAVSGAIPSPSGVAQFIR
                     NGVNYAKQAQSVLQTTISAVRVTQKMKDNPVVALTRVPGLMSGLGNISGALGKSVPAF
                     NALSESMPDAISLARTASEAATYVQQAQSALSGVDKRNIAGALDTVSGQLNAVGTAFN
                     RMSPGLSAMAARILTRSV"
     gene            56172..56387
                     /locus_tag="ISB30_000315"
     CDS             56172..56387
                     /locus_tag="ISB30_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011410692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988000.1"
                     /translation="MMFLEHVTRDGERWDSLAWQYYGDPLGYPRIIAANPHVAITPVL
                     PSGLLLLIPVIEAEEARTEEDIAPWLR"
     gene            56375..57529
                     /locus_tag="ISB30_000316"
     CDS             56375..57529
                     /locus_tag="ISB30_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HAP1988001.1"
                     /translation="MAEINSTAQVTSALTGVSDVLTPVFTLWYLQKNITSDIAPYVTR
                     VTWSDNIKNESDTIEVELDDTDGRWLDKWYPGKGDTLTLKMGYQGEKLLSCGTFSIDE
                     IEVSSPASVVSIRGVATSVNSALRTKTSRGFENTTLAAVAGRIARKHRLKLVGSIESI
                     RIDRVTQYAETDVGFLRRLASEYGYAVKVVSDQLIFSHLATLRSQEPVRQLKPQDVAR
                     FSLRDTINRVYKSAKVKHQKSSSKKLIVYEADGGTRESDKKLKGGKVTSADSLKVNSR
                     VSDPDSARIKADSALARHNEYQQNGSLTLTGTPQLTAGNKIELVSFGQLSGPWLITTA
                     RHAFDRNSGYTTELEVARGPVTRGKKQKTQKLTVYHPDGSTSTVIKEKKK"
     gene            57526..58122
                     /locus_tag="ISB30_000317"
     CDS             57526..58122
                     /locus_tag="ISB30_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HAP1988002.1"
                     /translation="MTGVTRQVGTVSAVDADRVQARVRLPECDNLRTNWLNVLQRNTQ
                     DNKDYWLPDVGEQVEVLLDANGEDGVILGAVYSDVDKPPFSDKNIRGTRFADGAEYSY
                     NRKTHTLIIRGGIEHIVIECGADVVLKTQKATIDAPETELTGDLRVRGKLIYEGGMAG
                     SGGEGVTATIHGNIEIKGNVHATGSMLSDGENSSHHSH"
     gene            58177..58524
                     /locus_tag="ISB30_000318"
     CDS             58177..58524
                     /locus_tag="ISB30_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HAP1988003.1"
                     /translation="MKTTSVFWQPALQAPGEIVRGLDDIRQAIQIILRTPRGSDPHRP
                     EFGSNLHLYIDWPVDRAIPHVVRESVDAIRRWEPRCQLMSVKPAVDGEHLTLRVSWKG
                     SDGQTRTQELLWR"
     gene            58515..59618
                     /locus_tag="ISB30_000319"
     CDS             58515..59618
                     /locus_tag="ISB30_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HAP1988004.1"
                     /translation="MALTEPDFIERDADKITAEMIAKYEADTGKTLYPAQAERLLIDL
                     WAYREMLVRVAVQEAAKQNLVAFAREPMIDYLGELVGVYRLAAQPATTTLQFSVDEAL
                     AIDVLIPAGTRVSASDSVIFATDTDVVLKAGLLLVNVTATCTEPGTAGNGWQPAQVSQ
                     LLDEIDNVNLLVSNLMASSGGSEQEDDDRLRERIRLAPESFTNAGSRGAYRFHAMQAH
                     PNIVDVAVLSPVPGTVDLYPLLSTGLPDGGVLTLVESFCSDEKVRPLTDTVRAKTPVK
                     VDYTIEARITIYRDQDARSVKDAANSAIQNWVASRAATLGRDIVPSQIISALSVSGVY
                     QVELVTPALRVVAENEWANCTAITLNMTGVSDD"
     gene            59611..60189
                     /locus_tag="ISB30_000320"
     CDS             59611..60189
                     /locus_tag="ISB30_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HAP1988005.1"
                     /translation="MTEPLQLPPPLEGDISLRTLGRLAGRLDNIDLSVLMVFLVDIVD
                     SSALPWLGEQFSLSGDGWELAESDDVRRMLIKAAIELHRYKGTPWSIREVIRRFGFGE
                     VDLIEGTGRLSYDGNRSYNGLFVHGDAAAWAVYRVILKQPITNDQAAMLRQTLAAFAP
                     ARCHLASLEYQSVAIRYNNTAIHDGSYNHGSS"
     gene            60192..61523
                     /locus_tag="ISB30_000321"
     CDS             60192..61523
                     /locus_tag="ISB30_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1988006.1"
                     /translation="MGNLNETEKWEENIYQLETSDPVLGGADGISNRAPRQLANRTKW
                     LKKKTEEAAQSLAEHVRSRNHPDATLTAKGFTQLSSATNSTSETLAATPKAVKAAYDL
                     AAGKAPASHTHPWSQITGVPAASLTAKGTVQLSSATDSQSETEAATPKAVKAAYDLAA
                     GKAPVSHTHPWSQITGVPAASLTAKGTVQLSSAINSTSEILAATPKAVKAAYDLANGK
                     QPADATLTALAGLATAADRLPYFTGADRAELATLTAIGRAIIAKGSIKDVLNYLGLGE
                     GSALPVGVPVPWPTATPPAGWLKCDGRAFTKEQYPVLARVYPTLRLPDLRGEFIRGWD
                     DGRKVDTGRKLLSAQGATLLRTAMLDYYNQDTTGTSGIVGMGFNNEDSITDLREGSFK
                     MPDGTTFSDPVVAMSDNGMQATILTSIRSGYAKGITVRPRSIALNYIVRAV"
     gene            61523..62137
                     /locus_tag="ISB30_000322"
     CDS             61523..62137
                     /locus_tag="ISB30_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP1988007.1"
                     /translation="MSNTAVLDENGIATVAGDITVYHYDEETREYTSSSVEYLALGVG
                     TPAHSCADAPPEAISGYVVCRTATLNGWEHVPDHRGETVYSTENGNPVLITQPGDYPA
                     DTTTKQPATPWDTWNGEAWVTDTERQRAAELEVARQQRQQRVKQAMASVDLINLKLRA
                     GRSLKPEETAKLNAVLDYIDELNALDISKAPEISWPEAPLALAG"
     gene            complement(62144..62605)
                     /locus_tag="ISB30_000323"
     CDS             complement(62144..62605)
                     /locus_tag="ISB30_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1988008.1"
                     /translation="MGEGSALPVGVPVPWPTATPPAGWLQCNGATFTKEQYPVLVRVY
                     PTLRLPDLRGEFIRGWDGGRKVDTGRALLSFQEGTIVSGFDDNDTGDISSLGSTQYGF
                     GDALTSNQLGVLNGKKWIFDASSKGAQRYDWWAYVSARPRNIAFNYIVRAA"
     gene            complement(62616..63056)
                     /locus_tag="ISB30_000324"
     CDS             complement(62616..63056)
                     /locus_tag="ISB30_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HAP1988009.1"
                     /translation="MNVGLGEGSALPVGVPVPWPTATPPAGWLKCDGRAFTKEQYPVL
                     ARVYPTLRLPDLRGEFIRGWDDGRKVDTGRDLLSRQDGTSFSHYAGNFDIGSGHSINN
                     YDQIVDNQPGFSRFSFAGPSRGDGVNYVTIRPRNIAFNYIVRAA"
     gene            63116..63688
                     /locus_tag="ISB30_000325"
     CDS             63116..63688
                     /locus_tag="ISB30_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP1988010.1"
                     /translation="MLIGYIRVSTNDQNTDLQRNALMCAGCEQIFEDKMSGTKSERPG
                     LKRALKCLKRGDTLVVWKLDRLGRRMKHLIALTEELRAKGVNFRSLTDSIDTSTPMGR
                     FFFHMMGALAEMERELIVERTLAGLAAARAQGRVGGRRPKLTKEQHEQIARLLQKGYD
                     RKRLAIIYDIGLSTIYRYHPVGTVITQPEM"
     gene            complement(63944..65227)
                     /gene="aroA"
                     /locus_tag="ISB30_000326"
     CDS             complement(63944..65227)
                     /gene="aroA"
                     /locus_tag="ISB30_000326"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP1988011.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLSGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            complement(65298..66386)
                     /gene="serC"
                     /locus_tag="ISB30_000327"
     CDS             complement(65298..66386)
                     /gene="serC"
                     /locus_tag="ISB30_000327"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAP1988012.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(66585..67277)
                     /locus_tag="ISB30_000328"
     CDS             complement(66585..67277)
                     /locus_tag="ISB30_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP1988013.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            67407..69167
                     /locus_tag="ISB30_000329"
     CDS             67407..69167
                     /locus_tag="ISB30_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAP1988014.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYSCRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT        16150 a        18542 c        18224 g        16318 t
ORIGIN      
        1 aaccaaatct ttatctttgt agcactttca cggtagcgaa acgttagttt gaatggaaag
       61 atgcctgcag acacataaag acaccaaact ctcatcaata gttccgtaaa tttttattga
      121 cagaacttat tgacggcagt ggcaggtgtc ataaaaaaaa ccatgagggt aataaataat
      181 gatgaagcgc aatattctgg cagtgatcgt ccctgctctg ttagtagcag gtactgcaaa
      241 cgctgcagaa atctataaca aagatggcaa caaagtagat ctgtacggta aagctgtcgg
      301 tctgcattat ttctccaagg gtaacggtga agacagttac ggtggcaatg gcgacatgac
      361 ctatgcccgt cttggtttta aaggggaaac tcaaatcaat tccgatctga ccggttatgg
      421 tcagtgggaa tataacttcc agggtaacaa ctctgaaggc gctgacgctc aaactggtaa
      481 caaaacgcgt ctggcattcg cgggtcttaa atacgctgac gttggttctt tcgattacgg
      541 ccgtaactac ggtgtggttt atgatgcact gggttacacc gatatgctgc cagaatttgg
      601 tggtgatact gcatacagcg atgacttctt cgttggtcgt gttggcggcg ttgctaccta
      661 tcgtaactcc aacttctttg gtctggttga tggtctgaac tttgctgttc agtacctggg
      721 taaaaacgag cgtgacactg cacgccgctc taacggcgac ggtgttggcg gttctatcag
      781 ctacgaatac gaaggctttg gtatcgttgg tgcttatggt gccgctgacc gtaccaacct
      841 gcaggaagag tctccccttg gcaaaggcaa aaaagctgaa cagtgggcta ctggtctgaa
      901 gtatgacgcg aacaacatct acctggcagc gaactacggt gaaacccgta acgctacgcc
      961 gatcactaac aaatttacaa acaccagcgg cttcgccaac aaaacgcaag acgttctgtt
     1021 agttgcgcaa taccagttcg atttcggtct gcgtccgtcc atcgcttaca ctaaatctaa
     1081 agcgaaagac gtagacggta tcggtgatgt tgatctggtg aactactttg aagtgggcgc
     1141 aacctactac ttcaacaaaa acatgtccac ctatgttgac tacatcatca accagatcga
     1201 ttctgacaac aagttgggcg taggttccga cgataccgtt gctgtgggta tcgtttacca
     1261 gttctaatag cacacctctt tgttaaatgc cgaaaaaaca ggactttggt cctgtttttt
     1321 ttatgccttc cagagcaatc tcacgtcttg caaaaacagc ctgcgttttc atcagtaata
     1381 gttggaattt tgtaaatctc ccgttaccct gatagcggac ttcccttctg taaccataat
     1441 ggaacctcgt catgtttgag aacattaccg ccgctcctgc cgacccgatt ctgggcctgg
     1501 ccgatctgtt tcgtgccgat gaacgtcccg gcaaaattaa cctcgggatt ggtgtctata
     1561 aagatgagac gggtaaaacc ccggtactga ccagcgtgaa aaaggctgaa cagtatctgc
     1621 tcgaaaatga aaccaccaaa aattacctcg gcattgacgg catccctgaa tttggtcgct
     1681 gcactcagga actgctgttt ggtaaaggta gcgccctgat caatgacaaa cgtgctcgca
     1741 cggcacagac tccgggtggc actggcgcac tacgcgtggc tgccgatttc ctggcaaaaa
     1801 ataccagcgt taagcgtgtg tgggtgagca acccaagctg gccgaaccat aagagcgtct
     1861 ttaactctgc aggtctggaa gttcgtgaat acgcttatta tgatgcggaa aatcacactc
     1921 ttgacttcga tgcactgatt aacagcctga acgaagccca ggctggcgac gtagtgctgt
     1981 tccatggctg ctgccataac ccaaccggca tcgaccctac gctggaacaa tggcagacac
     2041 tggcacaact ctccgttgag aaaggctggt taccgctgtt tgacttcgct taccagggtt
     2101 ttgctcgggg tctggaagaa gatgctgaag gactgcgcgc tttcgcggct ctgcataagg
     2161 agctgattgt tgccagttcc tactctaaaa actttggcct gtacaacgag cgtgttggcg
     2221 cttgtactct ggttgctgct gacagtgaaa ctgttgaacg tgcattcagc caaatgaaag
     2281 cggcgattcg tgctaactac tctaacccac cagcacacgg cgcttctgtt gttgccacca
     2341 tcctgagcaa cgatgcgtta cgtgcgattt gggaacaaga actgactgat atgcgccagc
     2401 gtattcagcg tatgcgtcag ttgttcgtca atacgctgca ggaaaaaggc gcaaatcgcg
     2461 acttcagctt tatcatcaaa cagaacggca tgttctcctt cagtggcctg acaaaagaac
     2521 aagtgctgcg tctgcgcgaa gagtttggcg tatatgctgt tgcttctggt cgcgtaaacg
     2581 tggccgggat gacaccagat aacatggcac cgctgtgcga agcgattgtg gcagtgctgt
     2641 aagcgttaaa accgatgaag cccgcttgaa agcgggcttt tttatgcgcc tttaccagac
     2701 aggcatttcg tcttgcaaga agggattatg caggcgctca taaccgagag tggataatgg
     2761 tccgtgaccc ggaataaatg tcacgtcatc ccccagtggc agcaatttat ctttgattga
     2821 agaaatcagt tgattgtgat cgccacgcgg gaaatcactg cgccctactc cgcctttgaa
     2881 aataacatcg ccagaaatga gcagccttgc ctgctcatta aaaaacacca catgacccgg
     2941 cgtatgcccg gggcaatgta acacctgtaa agtcacattc cctatgctga tggtatcgcc
     3001 ttcgttcagc caacgatctg gcgtcagcgg ctggcactct tccagaccaa acatacgact
     3061 ttgcgcaggc aagccttgca gccagaactc atcttctttt tccgggccaa aaaccggcac
     3121 gccgtaatgt tgcgccagtt ccgccgctgc gccaacgtgg tccagatgac catgagtcag
     3181 caggatctgc atcagtgtca ggccgctggc atcaacttcc tgtttgattt tttccgcatc
     3241 gccgccagga tcgaccagtg cggccagacg ggtttgttca caccagatca atgaacagtt
     3301 ctgggagaat gcggtgaccg gaataatacg atagttcata ctgcccctgt ttcgttaagc
     3361 gattactacc agtgccgtgc tggcccggta tcaatatgca caaagttact acgtgggtaa
     3421 tatcctacac cacctgcgcg catagataac gcggctttgc gaatattgct taacgcgata
     3481 ccttcaatgt ggaaatccat cgcctggcct ttggtgtgat agcttttctt cgctactcca
     3541 cggctgcggg cgcgtagttc attgttggta tcaatggaac gataaccgga aatgagttgc
     3601 accggtttgc gggtgcctaa caacccttgc aggcgataca actggtcgaa taatcctggg
     3661 tcgatggact ttattttgtt cgcgcggtaa tcgcggaaaa aatggttaag ttttgccaat
     3721 tcttcctgaa tatagcctct gccatcgaaa aactccgctt tgattgactc tccggtatga
     3781 agattattga gtgtcaaaat gcgcgggcgt ggggtagaga gtgttgcaaa cgccggggtc
     3841 ggcaggatgg cggcaccgag tgcaacgcca ccaagcgcca gcagtttgcg gcgattagcg
     3901 tcgaatttgt ccatgataat caagtctaca ggtcaatgtt atcgtttata tgcacttctg
     3961 gcgcacaaga ggcaccttaa ccgcccgtaa agaagacgtc aagatgactc aaccccagca
     4021 gtgacggggg ctgcagagaa tcgcccccga tcatgctaat tattacgaca actgatttcc
     4081 ccgaactgct tcatttacct gattaattgt tccgctttcg atacaatttg cgagctggat
     4141 cgcgcaggca gatcataatt gtaaatatct gtacgatact gggtacgacc atcagcacca
     4201 acaaaggccg tcagatagta gagattcacc ggaatcgact gccgaatatt gacgtaacgc
     4261 gtatcgccct gcttcagcgc atcagaaata cgtttgtcat tccatcctgc atcctgcaac
     4321 agcatattcg ccagatcgga agctttattc actcgtacac agcctgagct caatgcgcgt
     4381 gtatcacgtt tgaacagatt gtggttcggc gtgtcatgca aataaatggc ctctgaactc
     4441 ggcatattga atttatagcg ccccagcgag ttccgtgggc ctggagcctg ctggaaacgg
     4501 aacggtaaat tcgaggccgt gattgtagac cagtcaacct gccatgggtc aatcgcttct
     4561 ctgctgttcc agccgcgcat caccgtatag ccatggcttt cgagatatcc cggatcgttg
     4621 cgcactttcg gcagaatatc tttgcgtgcc agagttggtg gtacgttcca cggcgggttt
     4681 accactacgt tgttaagtgc actgctcatc atcggcgttt tgcgatcggg gcgaccgaca
     4741 atgactcgcg aatccagcac ctgattgccg ttctgatagt agaccagcga ataggctgga
     4801 atgttaacca tgatcccggt agaaagctct gttggcagca agcgcaatcg ctggatgttg
     4861 agtgccaaca cgccagcacg ctgggcgggc gttacgttta accagtcacg cgttgccggg
     4921 ccaatagcac catctgctcc caatccttgc catgcctgaa aacgtttaac ggcttccacc
     4981 agttcattat cgtaggcggc gcgaacggca ggcgcaggtt tactacgaga cgtagtttgt
     5041 ttgtccatca gcttagtttc tgctgtttca acagtaacag ccgatgggct gactaccgcg
     5101 tcagttggcg tgtcatcgcc aggtagcgta attttcggtc ccccgtccaa catgcctgta
     5161 cgttgcaata tttcgcgcaa cgccggtacg tcgttactcc actgtcctgg gcgcaacgtt
     5221 gctttaccgg tcagttgagg ccacggtttg gtgtcactga gtaaggccag taacgattca
     5281 tgcatcgccg catattgcgg atgctgcggt gccagtcctg caacaaacgt cggcaattga
     5341 cctttatcca gcgccagctg ccattggtta atcaccgaaa gcggcggcgt tgcaagcgca
     5401 taaggtttac tgctatatag ccagcgagtg cctttgaccg gaatatttgc aatgaaatgg
     5461 agatagccca tcatcgcatc agagagcacc acgtcgcgtg ccatcccgtt aacaccagga
     5521 tcggtcagta actctaccca tttattaaac tgcggctgga aaccggcaat cgccacctct
     5581 gccagctgtt gctggaaggc tttaacagca tcacggtttt cccacatggg ttgcatatcg
     5641 cgtgcggcat acaacaattg aagctggtta agataaaccg gcttataacc tgcgggtaat
     5701 tgagattcga tttgcgtgcg ggctttttca gccgcacctt caggcaatgg ctggatcccc
     5761 gccattattg ccgtggcttg cgcctgcggc agtgcctcgc cctgttcact gacagccacc
     5821 gggctgtcgc cagggattac ttcaggctca tcggcctgcg cattgaacag tggagcgaat
     5881 gttacggcca ggcacaaact gattgccgac agccgacgac cacacatcat attaagcaac
     5941 atcccttgcc ccctgttttt atcatactct gaatagccag gctgttattt tacgacttgc
     6001 ctgtacaacg ccaatactca cgaaagtatg acgagcaagc ctgttactca tcaggcttat
     6061 cttcagtata taaacgccga tacgttttta cataacctaa tgtaaagaag tttaaagaaa
     6121 agcgcgccgt aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg
     6181 cggcattgct gccgccttaa ttttaactcg cctgagaagg cgcttcgtca cttcctggaa
     6241 gcgtttcagg gagttgaggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat
     6301 tctggaagac tttacgtacc agtttatagg tggtgccttt ctccgggctg atattttccg
     6361 gcgctgcgat aatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatgg
     6421 aacgggcgtc cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt
     6481 taccgcgcag gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca
     6541 taccggtacc aatcgcctca ccggtcgaca atgcaccaga ctctgcacgc aaccagccat
     6601 cggaaccacg gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt
     6661 caccgatggt ctgcggcgtg cgctgcccca tatcaatctg cgggttaaga cgttgataca
     6721 gtttcgccag cgcttctgag aaggtcaggc ggttgctgtt aaacagatcc tgatgctgct
     6781 cgtgctgttc agagagcaca tccagcagca tggcgtgcgt ttcacgtacg ttcacgttca
     6841 gacgtacgct gttcacctgg ccaaacgata cgttctgcaa cccctggttg agcatacgga
     6901 tacggttctg ctcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga
     6961 tcgccagttt ctgttcacga gaggttaatt cttcggtcag acggctaagc tcgatctcca
     7021 tctgttcgat agcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac
     7081 gcagatgctg ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt
     7141 ccgacatccg cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac
     7201 ctaacgcctt atccgacatg gagcgcaaat catcagcgga gagataagcc agctcacgac
     7261 ggtgtaagcg acgctcaacg ccattatctt tcaccatgcg catcaccgca caccagcccg
     7321 ctttggcggt cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt
     7381 tacgggtcag gttgtccatc tccgcttcgc agaaggtaag cgctttttcc agttgattgc
     7441 gtcgtgaacg gttattgctc agttgcgcgt gcaactcgtc acggcgaata cgtgcccgct
     7501 cttctgcccc gctatcagca cgcacgccga tatcctgcaa ttcacgttgc agatcgttaa
     7561 gtagctcttt tttggtgtca tacgaacttt tcagcgaagc cagcacctgg ttgtactgat
     7621 tcaactgcgc tgcgtgtccg cgcaacgctt cacgagcgcg ggtacgctcc gcttccgcct
     7681 gttccagacg ttcacgcagt ttttcgttga gatcgctgtt accgctaagc atttctgccg
     7741 agtcagagta gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct
     7801 gacgggcatc gcgctgcatc tgctgagagt acgcgtaatc ttcttttaat tgttcgaact
     7861 gttccgggtc gctctgcaat accgaaacga tcggctccag tttcgccagt tgattgccaa
     7921 actgctgaac aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga
     7981 cgcgatccgc caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg
     8041 ccgtaacgcc ctctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat
     8101 gattactcaa cgcccgttcc agttcgacgc gacggctgtt cagttgacgg atttctgctt
     8161 ccgggtcaga ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat
     8221 gcagacgctg ggttttctgt acatcaaagg aaagcgtggc gaagcgttcg gaaagcactt
     8281 cccgctcggc atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca
     8341 cttccgggaa acgtgaataa cgccactgac gatcggcgat tttcaccacc accgcttttt
     8401 ccagctcatc aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat
     8461 agagatcttc cgggcaatcg gtcaagcctt ccaggtgttc ggttacctgt gacagatctg
     8521 gcaccacgat ggcgtggcgt gacgggccat acagtgctga gaagtacggc gcatcttcca
     8581 ggctaacgtc gtcataaatt tctgacagca gcacaccacc aaaacgctcc gccagcgcgt
     8641 tcagacgctg atcttcagaa ccacctggct ggcttaaacg ttcgatctct tcatcgacgg
     8701 cgtttttgcg cgcgcctact tcatcgcgtt caacaattgc ctcgcgctca cgctccagca
     8761 actgttgcag aaattcggtg acatcctggc tggaggtgaa ctcttcgccg cactgttcgc
     8821 tcaactggtt gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct
     8881 gaatgcgaga ctgcagctgt tcctgctcct ggcgcagtgc catccgctct tctcgggcgt
     8941 tagagacgct atcggaaaga gaggcaatgc gtgcttccag ctcctgatgc agagcttcca
     9001 gttcatcaat atcaaaattc ttgccctgac gtttgcagaa atctgccagc agacgctcag
     9061 cttcttgctg ctcacgcaga cgctgttcca gttcgcttaa gcgcatccgc aacggctgaa
     9121 cctgctctgc caggtgacgc tgatcgaccc cttcgcgcaa tagttcgcga gcgacatccc
     9181 acgcctcgtt acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa
     9241 actggctgtg cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcgg
     9301 tcgcttccag ctctttcgcc tggaaggttt ccagccattc ggcagcgctg tcggcggtta
     9361 agtccggcag atggcacagt tctttggcac gattaagcgc agcaatcgcc tggttatact
     9421 ggatcgcgcg cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca
     9481 gctcgtccac ttccagttcg gcagcttccg cacgagcctc gttctcttcc tggcgttcga
     9541 tggcttctgc caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg
     9601 cttcgtagcg ttcgatcttc tcctgctgac gcagtgcggt ttgcaccagg ttcaggtgat
     9661 cgctggccgc ctgataatcc gcttccagat cgccttcggc accgttgtgc tcagccagct
     9721 ctcgcgccat gtcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta
     9781 gctcgcgacg aaactccagg gccttatcga gatgaacacg gcgctcgttg gcgtgacgca
     9841 tgtagtccgc cgccacgtag ttggtggctt cgctgatcag atgcttaaac aggtcacggt
     9901 ccgactgggt gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca
     9961 tgtcctggaa cgctttccac accccactgt tttccggcaa caggtagtcg cgcagagaac
    10021 gagtaatggc gctggagatc ccgccataca gcgaggcttc gatcagacga tagaatttac
    10081 tacggtcaga cgctgagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt
    10141 ggtaatcggt aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt
    10201 ctttcagctc gttaagcggc agcacgcgcg cctggcgttc gttcagagtt tcggtcacca
    10261 gttgtgtcgg ctgcaccgac atcggcagtc cctgaatggc aaacggcttg atatccactt
    10321 tacgatcgcg tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtgacgcg
    10381 agttaatggt gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt
    10441 tatcgcgcga accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca
    10501 gatcggggat aagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac
    10561 cgccagaaag cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc
    10621 agttaatcag cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct
    10681 atctggctga ctctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc
    10741 ttcaccatca cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc
    10801 ggcgccgaag cggaacaccg attcggtaat gcggaacttg ctgctgtcgt ggcccataaa
    10861 ccacaccatg cctaaacgac gcagacggtt gagcgaagaa cgtacttttt cctgcaactt
    10921 ctgacggtca acgtctgaac cggttgaacg gttgttcacc agtttcagca gtttcgcttc
    10981 atcggccagg gtgagcagtt cgtcgtacag ttcctgttga gtaaaaatcc cctcattcgc
    11041 cagccgttcc gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc
    11101 cgacaagacg gaacgaggga tcagcgtggt ggaacgtggg cgtagataga agaacccttc
    11161 cggtgcacga ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa
    11221 atccatcagg aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa
    11281 ggcgctatcc agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccggcat
    11341 cacttgttca atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg
    11401 gctgccattt cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct
    11461 gatcaataac aatgcgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt
    11521 cgcgtaccac cagaccaaga tccaacggca cttgtctggt tttgtacacg gcaagttgtt
    11581 cttcgatgat cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtac tccagatcct
    11641 caggaagttc cccagtcact tcttcatcgc gcagtaccat ctcttcgtca cgcatatcca
    11701 gcagacgatc ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg
    11761 actgacgtaa ccgctgagca aagacgcggt ttttatccat atcgatcgca gtacgaataa
    11821 atttgtgtac gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga
    11881 taatacggtc gagtttgctc tgaagatcga acaccagacg atcgacaaaa tgcaggtcgt
    11941 catgggtcat cgtcgcatcc tgaatgcgta acaggttggc ctgcaattta tcgcctgccg
    12001 cttccagcgt atcctgcaat tcacgcagcg ttccggaagt ttctgaaagc aacaattcac
    12061 agctggaaat cgccgcccgc cagtctttgt tcaacaactg ggcgatatca tccttcacct
    12121 gctgctgctg ttcatccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta
    12181 ccgaatattt cagtggcgca taaacattgc ggtgccagtg aaattcatca ccgccctctt
    12241 cggcggcatc tgctgcgcgt ttgagctcac ccgccacaat cgacaattgc atagaaaggc
    12301 gcagcgtaga aaactcgcgc tgacgaatgt aatagtcagt gatgccgatg ccgagcggcg
    12361 tcagacggta aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt
    12421 gacgcaccat gtcgttgatc gcgttgttgg cgcgcacgcc gatggtttcg ctggtttgct
    12481 caaacgcatc actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat
    12541 ccagacgctc gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca
    12601 gcgagatgga gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa
    12661 attcactcat actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc
    12721 cagggtaata tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata
    12781 gcagtcgcgc tgctggtgct tctcacgcag ataatcatga aacacgcgaa cgcctgtctt
    12841 acccagaatt tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg
    12901 ataatctggc gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa
    12961 attcccggcg accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa
    13021 cacgccgcct ggacgcaaca ctgaccacag ggtctgtaat acgctgcggg gatcggccac
    13081 ccactcgagc accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac
    13141 atcctgagcg gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc
    13201 ctgttttgcg cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg
    13261 ctcggccatt ttgattgcgg tctgcccttc tccaccgcca gcatccagca cacgcagttt
    13321 ttgcgggcct atttctgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag
    13381 ctgccctttg gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt
    13441 gcgatcctgc attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt
    13501 ttgtcacagc cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct
    13561 atatggttaa aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat
    13621 atgttgctgc cacttccgct aatgttgttg attattggcg ctggcctggc gctgctgtgg
    13681 tttagccgtt ttcaaaaaac cgggaagatt tttattagca ttgggtggct ggcgctcttg
    13741 ttattgagtt tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg
    13801 acatggaata attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg
    13861 aacccgcagt gggcgccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa
    13921 ggtattcgct tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagct
    13981 aaaaccaata cggtgagcac agccgaagtc ggtgccagag ttgcgcaatc gctgggcgtg
    14041 ccgcgcgagc aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcagca
    14101 gtgaaacagg cgattggtga cgcacctttc ctgctggtga cctccgcatc acatctgccg
    14161 cgggcgatga tcttttttca gcaggaaggg ttaaatccgc taccagcccc ggcgaatcag
    14221 ttggccatcg actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtttggttg
    14281 atgcatagcg accgcgttgg ctatgaaacg cttggccgga tctggcagtg gctgaaaggt
    14341 tcgtcaggcg agccacggca agagtgattt tgatgccaga tcgaagttac gccgattaaa
    14401 tcgtccggtg ttgaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca
    14461 gatgaacgac tccgccactg gcgcgcgttg cagataactc cagagcaaat cttccgccag
    14521 agaattatcc attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg
    14581 tccgaccatc gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa
    14641 gttaccgtga atcagtacac aattgtcgtt aaagccttca aataatgccg ggagacattc
    14701 gcgagtgcga aacaggatcc gcttatcctg catcgataga ccggtgttat tgaactgatt
    14761 gagcgtggtc catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg
    14821 agtattgtcg accgcgccga cgcaaccacg actgtcctga cggtgccagg ccagtaaggc
    14881 ttcaacgatt tggtctttga gttgttccca gcgttctggt gttcgggctg gtgcctccac
    14941 cgaaacacca cgcattcgct ccagtagcag gacatcgggg ccggggtgtt cttcatgcgt
    15001 catcacacca taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt
    15061 ccatgccagt tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg
    15121 gtttccctgg ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc
    15181 aatacgactg agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca
    15241 gattaccctc ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg
    15301 aatgagatca attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc
    15361 caaaattggc tggagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcgag
    15421 atcttcaggg gtatccacac ctgtgccagg aacttcctgg gcgacagcaa cgtggatttt
    15481 ttcgccatac cacagaacac gaagctgctc taacatttcg atatgttcta acggacttgg
    15541 ctgccaggta acataacgac ggataaagcc tgctcggtag ccataaatac caagatgacg
    15601 caggaagtta tcgccaacgg tttcaaggtc ttttgcaaaa cgatcacgat cccacggaat
    15661 ggtggcgcga gagaagtaca gtgcgtaccc ttcggcatcg agaaccactt tcaccgcatt
    15721 cgggttaaac gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg
    15781 acgctgagcg aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc
    15841 accctgcaca ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc
    15901 caaacgttct gtacctgact gatgatcggc gcgcgtcata catacttcgc cgccagcggc
    15961 ttcaacagcg cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga
    16021 ttcgcgcgcg cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg
    16081 tttaccgggc agacgcgtgg acgcgtaacg cgcgggaata atgaccacaa aactcatgat
    16141 ttaccctcat cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg
    16201 cgcagcggga aagccaggtt gtccagtttg caaataagct cttgtttttc ctggttatac
    16261 caaagttttc cgttgcaaac cgggcaggca atgatttcaa gcagacgatg atccatagtt
    16321 cctccagatg gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg
    16381 tctatttccc agccggcgcg gcaatcagta aagaggcctt gcgagcagcg tcctaatgtc
    16441 acccgcgttg cctgctgcca gttagcgaaa tcagtaatcg cctgacgtaa ccctttttgc
    16501 agcgttgtcg ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct
    16561 ttctggcgat gcattttgga atccattcgc ccaactaatt gcccacgatg taataacggc
    16621 agaacaaaat agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta
    16681 aaatcaaaaa gctgctccgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt
    16741 accgcgctgt gcgttgcagt gagctttccg gcgagcgcac gctcgagtag cggcagcaaa
    16801 tcagcatgca gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt
    16861 tcagccctcg cttcacgcca cgccgccagt gccggacgtt ttagccggta gtaatctgcc
    16921 agccactgtt cgcgaaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct
    16981 gcttctgctt gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt
    17041 aaatcataaa cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat
    17101 aaaccttcca gatgtcgttt atgcggcttc cactcccacc agccgcttgc acctttacga
    17161 ggatgctcaa aatcggctga acgtaccggc cccctatcat gaatatgctg aattaacagt
    17221 gcaatttccg ccgcatgttc ctgcatccag gcgtctttat atttccagcc cattttttca
    17281 ggtgccagca tgcggtggcg aataagacga aaatcgctac gcggcataaa gcaggcttca
    17341 tgcgcccagt attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga
    17401 taatttccca gacgactgaa aagcaccaga tatgggctac gggcaacaat attgatggta
    17461 tcgatttgca gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga
    17521 cggtggggtt tgtttaacag gccttgtgca gcgagatgaa gattacgcgc atcagcaagg
    17581 gagaggtgcg gcagagacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag
    17641 ttgccagaag ccagcgaagt tagttgcgca agcagtttcg ctggttcatc acctgaaagc
    17701 tgtgcgtcta caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca
    17761 tctttttcag tcattaccag cgtttgtccg gcgcttacca acgcgctgac atccgcatga
    17821 ttcaaaaact gatgatcggc cagcggtaca catttttcgg gttgtacgcc acacatcttc
    17881 agcgtggcaa aaaagcgcgg cggatgccca atccccgcaa tcgccaccac atgttcaagc
    17941 tgagcaacgt cacaacgtgt accggtacgt aaattcaccg cctgacccgg cagcagatgc
    18001 atggggattt caccgctgcg ggggacaccg ccgttgacga ttaccgcatc aaccaacttt
    18061 aagcgccctg ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga
    18121 cgcacaccat caataacgac aatttccaca tcacgcgcca gacgataatg ctgtaaacca
    18181 tcgtcagtta cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcgtca
    18241 gaacgcacgg gagaaaccgc aacaggcgcg ccagtgcgtt gataaatcaa cacaggctca
    18301 tcacccgcct gtgctgtggt ggtatctgcc gacaataaca gcggataaga ttcagcctta
    18361 ccaccatatc cccgcgatac gacccccacg cgaataccgc gctgttgcag ctgttccacc
    18421 agccagacaa cgaccggggt ttttccgttg ccgcctgcag tgagatttcc aaccacgaca
    18481 accggtacgg gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg
    18541 ccactcacca ggccatacaa ccaggagagt ggcagcaata gccgccataa aggggactca
    18601 ccagaccaga ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc
    18661 cacggtgctc aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcgacaa
    18721 ccacgatttc gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac
    18781 ggtttttctg caactcatcc agtgctgcct gaatcgcacg ttcggattcg gtatccaacg
    18841 ccgaggtagc ttcgtccaga atcagtatcg ggctatcacg caacaaggct cgagcgatag
    18901 caatacgctg acgctgaccg ccagagagca acacgccgtt ttcaccaatg actgtatcga
    18961 gaccgttatc catcttattg atgaagtcca tggcgtaggc catacgcgca gcttcttcaa
    19021 tttgctcacg gctgtactgt tcagtccttg cgtaagcaat gttgttggca accgtatcgt
    19081 taaacagatg gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat
    19141 actcgcgcag atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgtg
    19201 tgattaggct ggcgatggtt gatttacctg aaccagagcg gccaaccaga gcaaccgtct
    19261 tccctgccgg aattttcagg ttgatgttac gcaatgcagg cacatcacgt cccggataag
    19321 taaaggtgac attgcgaaac tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt
    19381 cgtctttctc ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc
    19441 gctggaactg ggcgttaacg ttggtcagcg atttcagcgg acgcataagt gcaatcattg
    19501 aagagaaaac aacggtaatc gtaccggcag tcaggctatc catgacactt gggaagcttg
    19561 ccgcatacag aacaaacgcc agcgccaaag aggcgatcag ctgaatgatc ggatcagaga
    19621 tggaagaggc tgaaaccatt ttcatcccct gaagacgcat tcggttgctg actttatcga
    19681 agcgtttcgt ttccacttcc tgaccaccga aaatcaatac ttctttatgg cctttcagca
    19741 tttgttcggc gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc
    19801 gaaaacgctt cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa
    19861 tgatcgacag ttgccaactg taatagaaca tcatgatgaa caggccgatg atcgacgcac
    19921 cttcacgcac aacagtaatc agtgcgccgg aagaagaaga agcgacctgt tcggaatcgt
    19981 aggtgatacg tgacaacagc gtacccgttg actgtttgtc aaagaatgaa accggcattc
    20041 ccatcatgtg accaaacagg cggcgacgca tagtcattac cacctttcct gatacccagg
    20101 agatacagta gctggagata tagctggtga taccacgtaa aatcatcagc ccgatcacca
    20161 ccagcggcat ccacatcagc acggagcgat ctgttttacc aaagccatca tcaagaagtg
    20221 gcttaaggag cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg
    20281 ccacgatcag acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc
    20341 acgtagagag atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca
    20401 tattctaccc gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt
    20461 gctctctgag gctgctaatc caccagcctt gcgctgaaaa attgaccgtt atttgcccct
    20521 gatgtggagt atcaagccac ttataaccct gttgatgata gcgatattta actttgcttg
    20581 agggcagtcg ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc
    20641 gctgaattaa tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagtaatg
    20701 ttgcctgcat ttgctgccag taacggctta gcatcttttg ttcagcgggg gcttcaatat
    20761 caccggttag aagaatgcta ttcgtcccgt catcaatctt aaccacacag gaatggttat
    20821 ttcctttatc gttgctacct tgtaaaggcc agtgcgcgct gaaacgcaat ccttgccatt
    20881 gccacgcttc gccacgcaca cagggctgat gatgttccca gtttaacgga cttcggatcc
    20941 ataacatcgg ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat
    21001 gactcaaaat aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt
    21061 gttgcccact atccccttcg ggccaggcca gtcccgtgtc atagaggatc gctttgccgt
    21121 ttcgcgcaat gaccattgcc agcccctgcc cgacatcaag catgtacact tgccactcgt
    21181 caggtcgagg tttttgccac agcggcaagc acatcagcaa gcctacagcc acacacattg
    21241 ctggcaacgt tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg
    21301 ttagccattg ccaacgttca gcaatgttga tccacccttc cggcaaactc tttaaccccc
    21361 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgttcc agaataaacg
    21421 gcccgcttaa atgcacaacc atcgcggcga gaatcaacgg aaccgtgata aatgtcacca
    21481 ggggaattgc aaaaagattt gcaataaacg aggtcagact aatgccatga aatatgacaa
    21541 tttgcacggg cataagcagg agggtgattc ccagttgcag atggatgagg gaaacaaccg
    21601 cgcgcaatac cggcggcagc tgccactccg gacagggaaa ccactgatac caaaaaatca
    21661 gtgccgcgac cgcagcggca gaaagccata aactttgcga gagaatggca acaggatcca
    21721 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccat tgtcgtccac
    21781 ttaacttaag cattccccaa atggcaagcg ccaccacggt acgcaatgca ggaggttgca
    21841 ttcccgtaag ccaggcataa aaagcagcac agcagattcc accaattaat ggcatttgcc
    21901 agtggagcca gcacccaggc agaaaaactt gcccaccgcg aatgagtcct gcagccagca
    21961 acgccgcaaa agcgatgtgc aatcctgata tcgccattaa atgcgccgtt ccagtatcac
    22021 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aatccaagaa
    22081 taaccgcgtt ccacatatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
    22141 gcaggctaca attcggttca atgacacttg cctgcagaaa acggccggtg agcggttgat
    22201 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaactg
    22261 cacgaacttt gagtttcatt gaccatactt gtccggcaca aaccgcttgc ggaagatatt
    22321 cgccatacag tacgaggcct ggcgcaggga atatacgtcg accttgtaga tgagtaattt
    22381 gaccgtaatg agtggtcatg ccgtcagtag caatgatctc aacaattgca tcctgcgtcg
    22441 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgctaatatg ccccaaacga
    22501 aaaacagcaa cgtcagcgcg atataacgga cggttttaac agggataaat gccagtacac
    22561 aggcgaagag agtcagaaac gcaagggtta ctgtccctgg caattggggc aaaatcagca
    22621 acggaaaaat tccgcaaatt atgcatacgc cgactgtcgt tattttcatg caccctccct
    22681 gtttatagaa agtgtgcaac tttgttgaac ggttgtctgg tgggagtttt gcgttccccg
    22741 aggcacgttc agaagaattt aacgataggg tgaaaactgc tcaaaactct gcgaggcagc
    22801 ttccagttcc agattagaga aatgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa
    22861 gtttgagtaa aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt acctggttta
    22921 aagtgaggaa cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg
    22981 gtacgtggtg cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg
    23041 ccctgcgcaa gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc
    23101 ttggcgggaa tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg
    23161 attcctccgg ttccttaaag gcaaattaag cggctgctgt tgctacattg attaagggcg
    23221 gccgtagccg cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc
    23281 aacaaacttc ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg
    23341 aaagcttcag ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg
    23401 attgcatctt tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt
    23461 ttacgatcaa cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta
    23521 gcgtcttcaa cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca
    23581 gccagttcta cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg
    23641 cctttcttgt tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc
    23701 agggagatac gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct
    23761 tttttgtatt cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga
    23821 accaggccgt cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg
    23881 attttacctt caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta
    23941 gctttgcact gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc
    24001 ataacttcca ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc
    24061 caatccattt cggaaacgtg taccaggcct tcaacgcctt cttcgatttc aacgaagcag
    24121 ccgtagtcgg tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta
    24181 gcgatagcta cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt
    24241 tcgcggtcga acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc
    24301 ggatgcttaa cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc
    24361 agatcaacga atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct
    24421 tcctgcaggt tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca
    24481 cgacgagaaa caacaacgtt gttgcgcttc tgatccagct tgattacttt gaattcaagc
    24541 tctttgcctt ccaggtgcag agtgtcacgc accggacgaa catctaccag agaacctggc
    24601 aggaacgcac gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata
    24661 acaccggtaa cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga
    24721 cgtttagctt tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga
    24781 gcaacgtcaa cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc
    24841 tcagccggga tggcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg
    24901 atagcaacaa caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac
    24961 tcttcaaaga gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt
    25021 ccacctggct ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg
    25081 ggggtactgc aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg
    25141 ctttttcaat cacttgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg
    25201 ccggaaccag tggcgctacc gctcgattac gatcgcggtc gtcacgttct ttgatctcgg
    25261 ccaaaaggcg ctcaaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat
    25321 gcgcacgttc ttccgaggag gcgtcaagga aaattttcac tggcgcatca gggaatacca
    25381 ccgttcccat gtcgcgccca tcggcaatca ggcctggtaa ttcgcgaaac gcgcgttggc
    25441 gacgcaataa tgcttcacga acgcgtggga atgccgcgac ttgtgaagca gcattcgcca
    25501 cctcctgagt acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc
    25561 cgttggttga cacaaaacgt acatccagat gggatgccag cggcaccagc gcatcttccg
    25621 atgcaacatc aacatggtga tgtaatgccg ctagtgccag tacgcgataa attgcacccg
    25681 aatccagcag atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg
    25741 caccgcttgg gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa
    25801 aacaggcgta ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa
    25861 cgttgtgtgc aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag
    25921 cgggctggca gaagaccagc ccgaaagatg acaatgattt acttaccaga ggcgatacga
    25981 tcacggatgt gttgcgcacg ctctgttgat ggtgggtgag aatcaaacat ggactgggtg
    26041 tgaccgccat ccaggctagc gagtttttca aagctaccaa ccagcccctg ggtactgatg
    26101 ccacgtttct tcaacagatc aaaggagaaa tcatctgctt ctgactcttt atcacgggag
    26161 tatttagcat tgatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa
    26221 gccacaccgc tggtagctga aatggcatcg cgtgcggcca cgatcgcata agaagctttc
    26281 atttcagcca gcgagtgacc caacgcgacg tggcccagtt catggcccag aacgccttca
    26341 atttcgttgt cgttcatcat gtccatcagg ccactgtaga cacgaacaca accgttcgcc
    26401 atcgcccatg cgttgacgtc gctggtcata taaaccttat agttgactgg cgtgccatta
    26461 atattgttac ccaatgcttt ggcgattttt gccagacgtt tcccgtactt gctggatttg
    26521 cttgccactt tattgccgct gtccatttcg acacagccct gattggcgat tgctttagca
    26581 tcagcatcgc ttaatgttgc ggctttataa gcattcagac ctgagctgat agccaaatcg
    26641 gtattaatac cgtgcgtatt ctgacatcct gtcagtaagg ctgcagaggt cgcaatcgcc
    26701 agcaataatt tagtattctt ctgattagac ttgagataaa tgacagggaa taagtgagat
    26761 atttatggga agaactgggt caaaatggga agagactagg aggaatatgc aataacctta
    26821 cagtatgtga aaatcagacc ggcccgaaaa aaaaacagaa ctcgcgccag cctgattgca
    26881 gatattactc ttttttctcc gtatctgcat cgggttcatc agcaaatagc ttgccctgca
    26941 tccgatccag ttcttctttt cttacccgct taaccacgct gtagacccac tgaagcgaaa
    27001 caccaaattt gcgggccagt tcgtggtggt tgcgtccgtt aaactccctg aagatttccc
    27061 ggtcgcgctg actgaccttc cataccatgc ccatcgggaa ataaacgttt tgcccgcccc
    27121 agacctgcat cattcggttc gcgacggcct gaccaatctg gtcggcaact tcgggctcaa
    27181 tatcaataat ctcgcgaacg gtctcagagg tatgctgtgc cagttccacc aggagttccg
    27241 gccccttact tcgaaactga ttcaggtcgc tcatgtttga ctcccgcagc tctgcgctgc
    27301 cacttcttca gtttctcaat aacactgctt gcctgttcag tactgagcca gcgcagggcg
    27361 ctgatgcccg tttcccgctt gatccacctc gctaatgcat tttctgaacg gtcacgaaca
    27421 atgccggcag cagccatttc aagccatagc gcacgaattt tcctggactg cggatggtta
    27481 tcaagcggta aaccggagct ggcttttctg gctggtttaa cgcgaaagcc tttctttttc
    27541 atggattcca gcacgcagtt tagttgtgtg gtatccattc ctttggttga ggctttaccg
    27601 gtcagcccct gtaacatctg gcggtaggtg tcttcatcca tacccagttc attacgggca
    27661 atatggataa gctgtatcag gcgttttttc tgcatatcat ctcctttttt tcagtctgtc
    27721 ggcaacaatg tcggttgcag ctggcagggt gactggccag aacaacatga cggcggtcat
    27781 gtacaggatg taatgcgcgg tgtcgtagtg cctgccatag cccagggaac gatgaagtct
    27841 tgcgctgcat gatcctgcgt acatatacca gaaaagaaaa cagacaatgg tttcagtagt
    27901 cattctgaat acctccccat tcgatatgaa tattacgggc agcaatgaca gggtcgttat
    27961 tccaccatgc acctgacatg tatttttcaa cctgttcgcg tccggcaata acaccaattg
    28021 tgatccccgg cctgacgttc ttaaaaaagg cgcgggcaaa aaggtatctg gcagatattc
    28081 ggcaggcttt taatttccgg cttttacctg atagcgtaat catctggcct ccagtttctg
    28141 ttgttcctgc ccactgaccg ggcggtgcag tctgacgttc tgcccttcac gaaaccccgc
    28201 atagcgcgag gcgtcgccat tgcggcttct tcccggttta cgcgccctgg tggtttgcgt
    28261 ctgcgggtat ttatgttcca gccactgctg catcagttca cgctcatcat cggtcagggc
    28321 aaaggactgt atttcactga taacggccag cacccagcct tcggcaaact ggtctccacg
    28381 gctggtgcgg gtggcggttt ttattctttt gttctgtgca ctgatatact gctgacgcgc
    28441 ctttttcagc tgacgggcca gcacttccca ggtgtatgca gccagtgccg cacgttcccg
    28501 gttgccgtag aaccccacag acggatgcgt gccggggtga atgatggagt taacaccaaa
    28561 tacctcgcgg atgatgttca tcaggcccag catgtagcgc ggtggacgga gactgcctgt
    28621 cggccagtag tgactgatgg tttcatcaat atcactcata gcaatgtcgg aatgtgtgat
    28681 gccgtgaaca tccatcagtt tacgggctcg gcgcagtgcc agagcggcct cgtgcgggtt
    28741 gccggatgcg gccagcgcca gcaacttttt cagtttctca atgtgtttat tctggtctgt
    28801 cattgttctg tatctccggt atttttctgc cgtttccatg cccggacagc gtcggacagc
    28861 tctttcaggc tgtatgctgc tttaagcttt tcccataacc actgtgttgt tatgtgcatc
    28921 agcagggcag ccagggctgc ccccgcactg aggcatgtcg ccaggccagt aagaatcagt
    28981 gtccatgcgg tgatttccct gagtatgtca gccattgaat gcctcccggt tactcgttcg
    29041 tgtaaatcac gcccagtcgt gcagccagac gttccagttt tttctgtttg tggaagtcaa
    29101 tcagccggtc catcccctga aggcgcagtt gctctgtcat gatttccacg tctgccagct
    29161 ctgccgcgag gtcactttcg ctgccctgtc cgttcaggtt gcgggcggca ctggccgcca
    29221 gttcagcggc ctcttctgcc agtttcaggg cctgtgcgtc cggcccgaaa cgctgcaggg
    29281 ccagacggta gagggcggtg cgggtgagtt cggtgtttcg tgtcatgccg cgccctcagt
    29341 gcttccggct gacggtgatg tgcaggccgc cttctgcagt ggtttccatc cggtacggca
    29401 cctcatgctc tgccgtgtgg gtgagtgtgt tcatcagtac ctgcagggca gcctcctttc
    29461 cgttggtcgc cacaatggcc tgtgtggcca tgctgattag cgccgtcagg acgtgcttca
    29521 catcggtgag gtcgcggcat tcacactcgt tgacatagtg ttcaacaagg gttcgggtgc
    29581 gctgtcgtgc ttcctgtggg gtaatcattg cgagtcctcc ctgtcagggc gggagaattc
    29641 catgacgggc acgtctgccg aaaaatgctg gctgcagtac gggcagacca gggtgacgcg
    29701 taccgcaggg atgtggtatt taccggacat cacggcgatg gcgctgtgaa aacgcagggc
    29761 tgttatatcc ctctcgcact gaatacattt aaatatcatg atttaattct cctctgtttc
    29821 cggcgtgcag aagcccgcgg cgctgacgtc ggaataaaaa agaaaatgtt tttattaaat
    29881 aattaacgtg gtgtgtttac tgcacatcct gctcaaaagg aattattgaa aaatcctcaa
    29941 tgtcgctttt aatggaaata ccgggaatat ttttcacggc ctctttttca ttcaggatag
    30001 cgtctttatt tatttcctct tttacacgaa taaagcgctc aagccccaga cgtctcagta
    30061 attcaataac attatccgcc ccacggatac tgactgatgg cggacggttt cgccactgca
    30121 cctcgccggt ggtgaggtta gcgaacttca ccttcccgtt gccggtcagt tcatcacggt
    30181 gtgcctcaca ccatgtctga ataccggact gcagttcggc catgcgtttt ttcaggctct
    30241 cggtgagcgg ggcataacgt gcggtgatat cgccaatggc gtcattcatt tctgtttcag
    30301 ccctgaccag ttcacgttgt gcgtcaccga gtagtctgat tccctcaatg acctcttcac
    30361 gtgtccccgg cacccagagt gctgctgcgg ccttgatacg ttttgcacct tttgtacttt
    30421 ttgccatttt ttatgatttc tccagttgtg ctgattacca caaagattcc ggccacacga
    30481 cgcgacagcc gtgcagttcg aaaacgccct gacggaaata tcccctgtgg tcatgtccgg
    30541 tatacagata acaggccttt ccctgctcaa gcatgcgcat gcaatgcgca ctccgggaaa
    30601 cgcggatgac aggtttgtta cccctgatgg tgatgctttg tacatccgtg ttcgtcgcct
    30661 taagcgccat aatggctgac tgcactttgc tgatttgctg gttgatacct gtggtggatt
    30721 tcattattaa acccctttga caacgtcagc gttgacctgt ggaaccccga tttcagcggc
    30781 cagattcatg gcggctatta ccaggttact gacggccagc ggatacagca ggctgaccat
    30841 atttttacgg tgacttcccg gattgctcag gcgggcacgt atggcatcca ctgcaccggc
    30901 gtccataatg tccgtcagct gtttaccggc ccgttgcagt ttgaacgtca gaaattcttc
    30961 aaggttattg tccagaggca gaagttcgac cacctcacag cgctgaacga cttcacggac
    31021 ttccatattg cgttcagaca gttttgtcgc cagttccggc tggccaatca gcacgatgga
    31081 cagcagtttt ttgaaaccgg actccagctc aaaaaagcgt ttgaggtgtt tcagtgtcgg
    31141 aatgggcaga ctgtgggcct cctcaatcac cagaacgtgg ctgaaccccg cctggctgct
    31201 gtctttcagg acgcgatgca actggcgaaa acgggcgtcc tgactgcgtc tgatgctttc
    31261 cagtggtgcg atggtactga taatggcttc ggcaatagct gctgccttca gggttttccc
    31321 tttcacatcg ttgtcttcca tagcgatgat gtatggctcg ataacaatta ccggcgcatt
    31381 ctcgcggttg atacgttcag tcaggtcgcg gcgcagcgtg gatttacccg caccggactc
    31441 accgatgacg gccataaacc caccatgacg ggctgtctgg tacagcgcct cacgcacgta
    31501 gcgaatgtcc ggggtggtga acacatcatc agaaccctgc atggcttcgt cggcgaacgg
    31561 gtcacggaaa agaccaaacg cttttttggt tgctggaaat aacacctgtt ttttgagtaa
    31621 catattctct tcctcactga ggctcgtttt atctgtggta cccgctgtac ggggcgtggc
    31681 cgcgccctgt acagcatcaa aactcttcgt tgtatcaatc ccctgacttt ccagccagga
    31741 cgcaagacgc cggcgcactt ctccggggct ggtgcggggc cacgcgttat gattcacaat
    31801 ctgggccagc gtggcctcag aaacatcgac agctctcgcc accaccgcct gtggaatacg
    31861 ggcctctttc agttgctgct tcagtaccag catgtttccc tcctcagttg ccgttaacaa
    31921 tgctgataac actgctgcgg gccggtgtgg tcagcgtggc catgacttca tccagtgcgg
    31981 cttccggtac gccgtccggg tactgtgccg ttaactggcg gtaatgttcc ggcgtccagg
    32041 taaggccgtc ggcgctgaac ttatcgcgca gggctttcgc ggcctccaca tgagtcatgg
    32101 gacgttgttc agtgcgcggc ccgcgtacgt cagaggcctg accacgcttc ggcatatagg
    32161 ccggaagtgt ggtgtcgtcg atatgtttat acgggtcaag ccgcccgccg aacggcagcg
    32221 ccttcgcctt gcgtgcggcg gctgcatctg cggcgttgtc tgtaccggta accagcgctt
    32281 cggtttcttt tgccgccatc tgtgccgggg tttccggcag ggctttgtaa ctttcgccaa
    32341 ataccgccgc gccttcagca aagccaaact cgttctttct gacctcttcg accaggaaga
    32401 acgtctcgtg gccgtcctca ccggtcagaa ccacctgtgc cacatcgctg cgccatgggt
    32461 tacgggtaat catcagtttt tcaccaacca gtacgcccgg taccgttgat acgtcaaatt
    32521 cagtgccccg gaacgagaca cgaagttttg gcgtgacttt gcggagttct ggtgccgcca
    32581 cagccagttc acgacatacc tcaacggaag gcgctttttt cagctgctca gcagtaatct
    32641 tcagccagat atccgtgcgg gttttaccgt ggcggctgtg aacagccgtg gcgttaaagt
    32701 ggctgcgcca tttcgcggcc agcgcgttga gttcttccag actgtgaacc ggccggaact
    32761 tcagacccgg ctccagcttg cgttcgataa tgtcacgcgc cttttccacc tgtccggtgg
    32821 cgcgggcgtt atgcggcttg tgcgctatca ggtcgatgcc cagtgagcgg cacatgtttt
    32881 tcgtcatacc cgcggtgttt gccgagccgg ggtcgagata gagtattttc ggcacgccgt
    32941 gcagcacgtc tgcgccgcca cgctcctgca tggcgttgat aagcacagaa cacaggttct
    33001 cacctgattc cgcgcccgtc acatactcaa cgtaaatcca gccgctggca tggtcggtaa
    33061 tctcgtaact ccacacgcgg tcactggcga tacgggcaag gttagcgggc ttgttcttgt
    33121 agaacttcgc gctgtccatc acctgcagcc ctttatgtcc attgctcagg tagtaaagcg
    33181 tgcagagtga ggcgtcaatc tgccagacat gattgggatg cagactggcc acttcggtat
    33241 gtggtgcagg ggcatccagt tgttccgggt gcaggccata gttacgcaga gcacggctga
    33301 tggcatcttc agacaacggg aaaaactcac tggtggtttc atctgttctg cctgcggtga
    33361 taaagccgtt agcccgcagg gtttccactg catctgcgat ggaatagaga cgcttaccgt
    33421 tcttgcgggt ggcctcacgc agtgtggcag atatcagcgc ggcttcgtcg cgggtcaggg
    33481 cgctgcgccc ggcatcggcg cgttttttgc gtttatcagt cacagatacc tccttcagcc
    33541 tgcgcagcag agtggcgcgg gacatgccaa gttcagcaca ggcagcgtcg tatattgcac
    33601 cgcgtttacc atgccccgcg tcacgtgccg cgcgggctgt ttcaaccagt ttttcagtca
    33661 gtgccggggt catagcttgt cttccctgtt atccgattct ggcggcaatt tttctggcga
    33721 ggtcgcacag tgtttcgcca tcgcaggttt tcaccacggt atttaccggt tgcggtgcaa
    33781 ctaccgggcg taatgctgac tgattctgca gacgtcttat ctgctgcatc agcatcacct
    33841 ccatcgtcag tacaaacggg tgcgtctcct gtgcgggtac gcagatttca ccggaagggg
    33901 tcaccacgcg gaaaccctgc accatctccg gcagcagatt acacagtgcg tgccccagtg
    33961 cctccgggct gtccatcagg gtattttgtg tgttattcgt attcattact gcatatcctc
    34021 cggtcggttg atttcaggtt ccggttcggt cagccaggcg ggggcatcgt tacccgatgg
    34081 agcggcctca aggtcaaacg tctcacgtaa tgcatgaaca ctgcgttcaa tctggcagac
    34141 cagaccggcc atgaaatctc tgggggtatc gatcatgttt ttggtgcagt attcacacag
    34201 ggtctcaaaa acactggaca gccggacggc gatagccgat tcggcctcaa ccgctaatgc
    34261 cgtcacttcc gcccgcaatt ttttcagttc ttcgtctggc ggcggaggtt gcaggcgcga
    34321 tttcttctcc agttttgtgg ccagttcgtt gattttttcg tcctttccgg caagcacctg
    34381 ttgctgagct gcgttggttt cgcgcgcttc acgcagggcg gccttcagtt cgcggctggt
    34441 catgcgatca atatcatcaa gcgttgcacc ggcgacggtt ccaccatcag ctaatgcgtc
    34501 gagttcttcg tcatccagaa ccatcagttc atataacttg gtttttccca aaacgctcag
    34561 cgctgagcgt tttggctgtt catctccatt gccgagaaat ttcacgctgg cctgcatcat
    34621 gcgacgagct acctgtggtg ctaatccgag gtcgttttcc agaatatttg tgaaatcacc
    34681 atgcggttca ttttctttta agatgatcag ccgcttaccc gcttccaaca tagattcagc
    34741 actctgcgcc atataaaaac gtgcttcgtg aacgatgcgt tcacgttcat aaggcagtcc
    34801 atcaccaaac tgctgcatga tctccatgcg atgctgtgtc atggcattca ggctgacgtt
    34861 gagatcatcc gacagcggta catcggcgtt cagttcagtg ttaatcggtg atttggtacg
    34921 tcccattgat tactccttac aaacgactac ctgaaatgac acgttggtta atttcgttaa
    34981 tacgatcctg tgcacgtgcc atctcgttac tgtgggccat ggcgatctgc agtaactgga
    35041 tgcccggtgc aaaacgtccg ttctccagtt tcagagccag cccttcttca atgagggtgt
    35101 tgagtgcccg gttgatgttt gccggtgact cgtgcagtgc tgtcgccagt tcaccgttag
    35161 aaataccgtt cagggcatga ccgcgcagcg ctttgagtac ccggaggatg cgactgccgg
    35221 agcttgaaac attcgtctta ctcatctgca tacctctttt gcgatatgtg ataacctttt
    35281 acaaagactc aaaatattta ggccgcctga gctgttgatt tcagtcccaa cttcacggcg
    35341 atttcatggg atttaccgta acgggctttg gtctgcccgt tgagaacgcg gtatacctca
    35401 ttgcggttgt agccgttttc ttctgcccag cgggtgaaag tgatcccgcg ctggcggaag
    35461 agagctttga cttgttctgc agtcatcgtt gtctcctttg ttgatgcaat gatgtttgcc
    35521 ttatatgtgt tagattattg ctcataatta tgagcatatc aagatagaga ttcactttta
    35581 tgagcaaaga tataggagag cgcgttattt ctgtgaggaa gaaagcaggg ttgaaccaga
    35641 gagaatttgc agctcgttta ggtatttcta atggtggtat cagtcagatt gaaaatggaa
    35701 aggcgatgcc tggaggggag tttctgttaa agatgcatca agagtttaat gttgatgtaa
    35761 attggttgct cactggaatg agcacagatg gtatgactag aaaccagaat caaatttccc
    35821 cagaaaaaca aaaactaata gatacttttg acgacatgac accagaccaa caacgctcat
    35881 ttcttgagat tgggcgattc tatactcagc caaaaccagg aaaaatcgca ggctgatact
    35941 gacccggaca caaaggacag gtggttgatc tggatgcttt cagaaaacat cgccagtgac
    36001 atagtggaag ttgtttggtt taccggaaag cattgtgctt tccggttttg tttcagaagt
    36061 tgcagattag cagttcacgg cgcggcgtgg gtttacccgc cagactgtag ttgatattca
    36121 ctgtctgtat gttcagtcca gtgaacacct cgcgcatctg cgggatgtcg ttcaccgaaa
    36181 ttatcatttt tcctctgatg caccgggcca gctctgccag ccgcgagtag ttcccttcag
    36241 gaaaatccac accatagcct tccgtaccca ggtacggtgg gtcgcagtag aacagcgtgt
    36301 gcggacgatc ataacgctca atgcactgtt gccagtccat gtgttctatg actgttctgg
    36361 aaagccgcag gtgtgccgct gacagttctt cttcaatacg tagcaggttg aagcgtggcg
    36421 gggatgtggt ggaggtaccg aagctgtgct ctgccacctt gccgccaaac gcctgcttct
    36481 gaaggtagta gaaccgtgcc gcacgctgga tatccgtcag tgtttcttcc ggggtgatct
    36541 gcagccattt gtagatctga cggctgacca gcgcccattt gaactggcgg acaaattctt
    36601 ccaggtgatg ctttaccacc cggtacagat tcaccagttc accgttgata tcattgatga
    36661 cttcgatctt gccgggtgtc ttaagaaaat agagcgctgc tgccccacaa aacggctcca
    36721 cataacattc atgcgccgga aacagcggca gaatgtgttt tgcaaggcgg cgtttgccac
    36781 caatccacgg cacgatgggt aatgtctgca ttttcataat ctgcaagcct tttacaatta
    36841 ttaaaaaata tggcagtctg gtcaggtctc gcgagactga ctgaaccttg gtcggctcac
    36901 agcgcattcc tgtggggcga tgaccagccc ggctttcggg tgccgggctg gtcgttcttt
    36961 catgatgcgg cagggatggc gggatttctg ttaatgtggt ttagggatta tccggggcga
    37021 ttttctgacc ttatccggtc cagggcataa gtaatacctt ctgcaaaaac agaagtatca
    37081 aactgctttt gtattccctg aaaatgaaaa gcggcacgac agacactcag ttctccgcgg
    37141 tataaccgga taatatcttc cagaacattg ttcactgctt cagcacggcg ctgatgttct
    37201 gttaacgtta atcggggatt catgatgcac tccttaaatg atgattttta tgtactcacc
    37261 ggcaaaatat tctgccgatt actgaacacg catccggcac ctcaggtttt gatttattct
    37321 ggttttttcg atatcccgga ccaggttgat cacgaccaga aaaagtgccg gtgttttatt
    37381 gatacgctga tatggcttcg ggataacagc tatattcgtt actctcagtt gctggctgtg
    37441 ggagcggctg atgtcgtcct cacggaaaaa acgctctgtc tgctcaatgc catgccgacc
    37501 tttctgcagc cccggcccgt accatttaaa gagtcacttc tggatgcagt aaaaaccggt
    37561 gaaaatgcag tcatcgctga atgcgtatcc cggattatgc gttacgtgtt taatgaacgt
    37621 aagtgaggtt tatccgctgt ctgcataatc ccgatagcca gtgcgagggg aagtaattcc
    37681 ggggaaactc tttcaaacag cgatgcccat tctcctctcg ccatcactct gtccagcccg
    37741 ccctccagtt gcatttcagc aatcttcctg atttcccgga acaacgccgc tttgatctct
    37801 tcatgctcag ctttattttc cggtgtcaga aggtattcgt cgaagaaccg gggaggctgg
    37861 ttaatcggga tgtgttcctg tttgccgtac ttcggggacg aacaacattc tgttgactga
    37921 ccagtgagtt tacgtaactc cttcatgagc agctcctgat cgtaaactgt catcagtgcc
    37981 gcaatggtgg catgcagtgc atcgggcagt atccagggtt gccggcccgt ggggcatata
    38041 acatccagca gctcattcgt ccgacagata cgctcctcgc cgtatgacaa caggccctgt
    38101 cccgtgtatt cctccagcct gatcactgtg ccagcccgtg gtttctgcca tgtgctcaga
    38161 tcatccccac tcatacggcg ctcaccgtcc acgtctgcgg cgtcgacaat gacgctgttt
    38221 ccgaattcct gtttcaccac ttcctctatt cgcgccagta cgtaactttt accgctgccc
    38281 acaggaccgg acacggtgac agtgatgacc ggattcagtg tctgcgtcat atcgtattct
    38341 cctctgtgaa taattcatcg tttatcctgc acatcccgta tccccggctc tcttaacgcg
    38401 ctttaaaatc cttcgcgccc tgtatttgtg atgctgtctc caccagataa ggagacacac
    38461 atgaaaaacc tgaaaaaatt cattccccct gttaaaaaac ctcgcctcag cggctggctg
    38521 ctgacctcag tgctgttgct gggcaccatc gctctggtct caccacagca gctgcctgtt
    38581 gtgatctaca agctggcact catcacgctg gcagcagtgc tgggttactg gcttgaccgt
    38641 tcgctcttcc ccaaagcccg tcccggtcag tacctgaaac atgacgacag gctgatggct
    38701 gaagggcgtt tcccggtaca gaccggcctt cacctggtgt tttctgctgc gttaatccgc
    38761 cgtgcactga ttgttgcagc ggtctgcctg gctgttgcga tggggcttta atcatgaact
    38821 ggccgcagat cacctggatt gtatgcatgt ccctgaaact ggctttcgaa gcctttcggg
    38881 tcttcatcag aaccgaacgc ctgtcagtcc gcgccgggac ctttctggtt catatggcat
    38941 gggtattctt tgtcgcaatg ttgctctggt gtggcggctt tttcagccag gcatgcgcag
    39001 cacaacctcc gcaggctgcg ctgcagtatc gcgatgatgt gatccgtaat gcccggcttg
    39061 aatggggact gtctgcgccg gtggccgatt tcgccgcgca actgcatcag gaaagcggct
    39121 ggcgacctga tgcgatctcg ccggctggcg ctcagggact ggcgcagttc atgcctgcca
    39181 ctgccgactg gataagccag ttgataccga tgctgagcag tcgtgagccg tttaatccgg
    39241 catgggctat ccgggcgctg gtcagctatg accgctggct gtggcagcgt gtcagcgccg
    39301 ccagtgactg cgagcgtatg gccatgacac tgtcgggcta taacggtggt ctgggctggg
    39361 tacaacggga caggcggctt gcatcacaga aaggtctgga cagcacccgc tggttcggac
    39421 atgtcgccac ggtgaatgcc ggacgcaatg cggccagctg gcgggagaac cgccattatc
    39481 cgcagcgcat cctgcgcgaa ctggcaccgc gatatctcac atggggaggc agcagttgtg
    39541 tggcatctgg ttaaaaagct gccgtggcgc ggcattctgc tggccattct tatcaatgcc
    39601 tttctggtcg gcctgtatgc catgggatac agaagtggtc atgactctgc aaagcgtgac
    39661 ggtgataccg cgctaagtca gttgcagtca gcatttgacg cgtacaaaac ggagcaggca
    39721 acgcttgaga atgctgcgct gcgggcctgg gccagacggt atcaggagca ggtggccgcc
    39781 gggcagcggg ctgaagccgg ttatcttgag cagattgctc aactggagag ccggaacaaa
    39841 caactacagg ggcaaattaa cgatgtcaca cagcgctgga ttgatgaaaa aggtaagagt
    39901 catcccattg agtgcgtgtt tactcgcggt ttcgtgcgcc agtacaacgc cgcactcgga
    39961 tatgacaacg catccgtcga caccggtcat tcagactcag ttgccgccac tggcacccgc
    40021 tctggcacag cgaccgggca acctgaaacc actgacaccc ggttacgcga ttcgggtgtc
    40081 tcccagcgtg acgttctcgc caacatcatc gacaacgcat ggcaatgtcg tcgctggcgg
    40141 aaccagataa acgcgctgct ggatgaacgg gaaggattac agaaatgaca ctgcaggttg
    40201 aattctggac ggtggtgagt tttctgctca ccttcatgag gtttgtggga gggctcgcca
    40261 aatggttgtt cagtaaaaca gaagaacgcc aggcggcacg attcgcctcc cttgaacagg
    40321 ccctgcaaca atccgcctcc aactggggcg agctggaaaa agaatttatg cgatttaaag
    40381 cggatttacc gctgaattat gtccgtcgag aggattatat ccgtggccag acagtcattg
    40441 aggccaaact ggacgcgctt tataacaaac tggaagtggt acagcagtac cgcaataccg
    40501 gaggtcaata atggtcgata ttacccgggt acgccgcgaa tccctgcgct ggagtctgct
    40561 ggttgccctg aacaagaccc gcccttacac cgccagcgag acgctgctgc tggatgtgtc
    40621 ccgtgccatc tacccggaca ccacaccgct ggaactgcgc cgcgaactgg attatctggc
    40681 tgaccgtaaa atggttgagc tggagaaaaa accttctggc gactggtttg ctgacctgac
    40741 ccgcctcggc gtagacctgg tggaatacac cgtggaatgt ggtccgggta ttgcccgccc
    40801 ggaaaagtac tggagtgaat aatggccaga cgcagcacaa tagaaaagct gccggaagac
    40861 gtgcgccgct ggcttgaacg ggcgctgact gaatccggct tcagcgggta taacgagctg
    40921 gagtcactgt tgcgtgagcg ggggtacgtc atcagcaaat ccgctatcca tcgctatgga
    40981 cagaagattg agcgccgcta tggtgctatc cgtgcggcaa cagaagcggc ccgcatgctg
    41041 accgaaggcg cagcagacga tcaggatgcg cgttcggagg ctgtgatagc ccttattcag
    41101 accgagctgt tcgagagtat tgtccagttg caggaggcgg aagaaggcga agtcgatcct
    41161 aaagaacgcg tggccctgct gtcgaaggtg gcgaagaatg tggctacgct gtcccgcgcg
    41221 tccgtcaacc tcaaaaagtt ccagtctgaa gtacgggcca gagcgcagca ggcagccagc
    41281 aacgccgaga aaattgcccg taagggggga ctgtcaagcg acgcagtaca ggcgcttcgt
    41341 cgcgagattc tggggattgc cacatgacaa aatcatccgg agtgatttta aacgccgctg
    41401 gcggcggccc cgaagggatg agtcccatgg acgggaggaa taaccttgct cccgttttgc
    41461 ctgatacctc ggcgctggat gcccctcccg ttctgttgcc ttaccagcag cgctgggtgg
    41521 cagacacctc tccgcttaag gtgatagaaa agagccgtcg taccggtatt acatgggctg
    41581 aggcatccga taacgtactg accgccgcct cttctgcgcc agcaggcggg atgaatgtgt
    41641 attacatcgc ttataaccag gacatgaccg tcgaatacat tcaggcgtgt gcgatgtggg
    41701 cacgggcatt caactatgcg gccagtgaaa ttgaagaagg attctgggaa gaggacgacg
    41761 acgacaaaca catcaagact tacaccatca aatttcctga ctccggcttt cgtattgttg
    41821 cgctctccag ccgcccgtct aacctgcgtg gccgtcaggg tattattgtt atcgacgaag
    41881 cggcgttcca tgagcaactg gacgaactgc tgaaagcggc gctggcgatg cttatctggg
    41941 ggggaaaggt acgcgttatc tccacccatg acggtgacga caatccgttc aatacgctta
    42001 tcggggatat ccgtgccgga cgtcagggag gcagcataca tcgcatcact ttccgggaag
    42061 ccgtatctga ggggctgttc cggcgcgtct gtctgcgcac cgggaaggaa tggtcggagg
    42121 catccgagca ggcctggatg gcatcggtgt acaaattcta cggtgccggc gcatccgaag
    42181 agcttgactg tattccggcc aacggtggcg gtgcctggct gtcccgtgcc ctgatagagt
    42241 cccgcatgtc cgctgatacg ccggtattgc gtctgacctg caaggaaggt tatgaactgc
    42301 tgtctgatga ggttcgcttc cgcgagacgc aggactggct tgatgagtat ctgaaaccat
    42361 tgctggaggc actccccact gatgcccgct ctttcctggg gcgcgacttt ggccgtagcg
    42421 gtgatttgtc ggtggactat cccctgctgc aggagaagaa cctggtacga cgcgtgccat
    42481 tcgtactgga gctgcgtaac gtgccgttca gacagcagga gcaaatcacc tggtatctga
    42541 tggatggcct gcccggtctg ctgggtgcag cgtttgatgc ccgtggtaat ggtgcctatc
    42601 tggctgaata cgccatgcag cgctacggct ccggccgggt tcagcaggtg atgccaaccg
    42661 aaggctggta ccgggagcat atgcctccgg tcaaagctgc actggaagac ggcaacctgg
    42721 tggacttacc aaaggatgaa gacacactgg atgacctgcg ggccgttcag gtggtgaacg
    42781 gtgtcccccg cgtaccggag caacgctcaa aagcaaaggc tgacggtggt aaacgccacg
    42841 gggattcagc catcgcactg gcgctggcgt atttcgccag ccgtgaaatt aacaaagggc
    42901 cggtgaaggc aagctcacgc cgtcgtcgtc aggcggcccg tatgctggag ggattctgat
    42961 ggcgaggggt atctgggttt cacccgatga atttgttgct ttttctgagc ctcagaaatc
    43021 actgaccgcg cagattgcct cccgcagccg cgcgatcgac ttttacggac tgggcatgta
    43081 tctgcccaat cctgatccca ttctcaaggc tcagggacgg gatatccgta tctaccgcga
    43141 actgcgcacc gacccgctgg tcgggggctg tatccgcaga cgtaaagcag cgctcaaatc
    43201 gctggagcgt ggactggagc gtggtcacgc ctctgcccgg gtcttccgtt tcatccgcga
    43261 catgctcgac gatctggatc tgtcccgcat catcggtgaa atgagcgatg ccgtgctcta
    43321 cgggtatcag ccctgtgaaa tcatgtgggg ccgttcggtc aggtcgtggg cggtgacgga
    43381 tattgtcggc aaaccgcctg agtggtttca gtttgatacg gacaactgcc tgcgctttcg
    43441 ggcgcgtgat gcgggtgtgg agggtgagct gctgtcaccg tcaaaattcg tggtgccggc
    43501 acaggatgcc tcgtatgaca atccttacgg tttcccggac ctgtccatgt gcttctggcc
    43561 ggtcgccttc aagaaaggcg ggatgaaatt ctggctccgc tttgccgaaa agtttggctc
    43621 cccgtgggtg atcggtaagc acccgagggg cgcaaatgat gcagagattg aaaaactgct
    43681 ggactccatg gagcagatgg tggaggacgc ggtggccgcc atccccgatg acagcagtat
    43741 tgagctcagg gcagcggatg gcaaggcaga cagcagcgag gtatttcgcg agctgatcac
    43801 actgtcacgc agtgagatct ccattgcatt actcggtcag aatcagacca cggaagcgaa
    43861 cagtaacaag gcctctgcac aggccgggct ggaggtaacg gctgatatcc gcgatgcgga
    43921 tgcggacatc attcaggcgg cagtgaatca ggtcatcaga acggtggtca ccctgaactt
    43981 cggcgatgtg ccgtgtccgg tctgggccat gtgggaacag gaggccattg atgacacccg
    44041 tgccacccgc gacgaaaaac tcacccgggc gggtctgcgt ctgaccccgc agtacttcaa
    44101 gcgtgagtac caactgcagg acggcgatat tgacgagaca ccaccgtcgg aacgccagaa
    44161 taacatgctg ccgctgtcat ttgccgaggc gattgatgct gatattcagg ctcagcagca
    44221 gcttgacgac gcgctggaca ttctgatgaa cggaggcgcg ttaaatggca cgctggaacc
    44281 cgtcctggca cctctgttca ggcgggtcga aaacggggtt aacccgtctg agctgctggg
    44341 cgaactggcg gagctctacc ctcagatgaa cacggacgat ctgcaggagc ggctggcccg
    44401 cattctcttt gtggcaaata tccggggacg tctgcatgag cgtgacaacg gctgaactgg
    44461 cgtactgcat gacgcttccc ccgaagcggg caatcaacta cctgaagtcc aaaggatatc
    44521 agattacctg ggactgggaa gaaatgtggc aggaggccca tgcccgcgcc tttaccgttg
    44581 ctaaagtgac ccgcctggat atcctggaag atattcgcgg ggcactgcag caggctgtcg
    44641 atgaaggaaa aaccgatcgc tggttccggc aggagctgga gccggtgctg aagcgtaagg
    44701 gatggtgggg accacgtgac acgactgacc cggtaacggg tgagccggtc accattcagc
    44761 agggcagtcc gtggcggctc gataccatct ttcgcaccaa tatgtccgta ctctacagcg
    44821 ccggtcgttg ggcggaacag atggaaaacg tcgacgacag gccgtactgg atgtataccg
    44881 gcatcaacga cagccatacc cgcaggagcc atctggcgct gcatggtctg gtgctgcgct
    44941 gggatgaccc gttctggcag gcattttacc cgccgaacgg ctggcgctgc cgctgtagtg
    45001 ttattgccct gagtgcggcg gatgtacgtg cccgtggcct gaaggttatc agctccggct
    45061 ctgccatggg ccaggaactg aaactggtct cagagaaaac cggcgaaatg cggaacgtgg
    45121 ccacctttaa taccggcacc acgaaggtga ccaccgacgt cggctggtct tatgctccgg
    45181 gggcagcata ccgtcccgac ctggcccgct atcagggtac gcttcagcca ctggcacaac
    45241 aggaactgag aggataacaa tggcttccga taacctggtc agtatcacca ttaacgataa
    45301 gtccctgcgc cggagcctcc gtgcgctgga tcttgctgcc acagacctgg agcccgcgat
    45361 gcgcaaaatc gccggaaccc tgctggcgga aacacagttt aactttcttg atgaggggcg
    45421 tccggggtgg atgccctcgc tggcagcgga agaacgtgac gggcagacac tgcaggatac
    45481 cgggcgtctg atggggtcag tatcaaccga ccatgacgac cggcaggctg ttgtggggac
    45541 caacgttgtt tacggtgcca ttcaccagtt cgggggtaaa acggggcgta atgagtctgt
    45601 tgaacttccg gcccgcccgt tcctgccggt gacgggggat ggagaactac agcctgaagt
    45661 ggtaatcccc atcctcgata ccattgtccg ccatcttgaa tcagcggccc gtcgctgagt
    45721 tttctctctt caggcgggtg atttatcatt gccagcgaat gaggggctgt attaccttta
    45781 taaaggcttt acagcctctg ttttataacc gcctccggtt caccgcattg ctttccctgt
    45841 ccttctcccc tgatgttttc taaagcagat taaaatcgcc gggcctgcat ttctcacaaa
    45901 ctgtctccga caacataacg cgggacagca aaatgtcagc cattcacatt tttaaagccg
    45961 gtactcatac cgatatgcac ggcaaaaaac tgccgttcac gccagacgat cttgccgcct
    46021 gcgtgaaagc ctatgacccg tccgtccatg aagcaccact cgtgattggt catcccagaa
    46081 cggaagaccc ggcgtggggc tgggtgaaag ccctgtcgct cagcggcgtc gatctgatgg
    46141 cagagcctgc ccagctggac ccgcagtttg ctgagatggt caccgacgga cgattcaaaa
    46201 aagtgtccgc ctctttctac ctcccggatt caccgtccaa tccgaagccc ggcgtgctct
    46261 accttcgcca tgtgggcttt ctcggggcac agccaccttc cgtcaagggg ctgaaacagg
    46321 tgtccttcag tgagcaggaa gaaggtgtgg tggagttcgc cgactggcag gccatcacga
    46381 atgcctccct gtggggaaag ctgcgcgatt ttctgatcgc ccgcttcagt ctggacgagg
    46441 cagaaaaagt cctgccggaa tggcagctca acagtctgcg cgaagaggcg taccgcgaca
    46501 cactgtcgca ggatgcagca ggtgcacaat tcagtgagac aggcccgggg ccgtcttccg
    46561 caagtaacga ggaatcatcg atgacaaaag aagagattga agcccttcag gaggagaacc
    46621 gccgcctgaa gcagcaggct gctgatcgcg atgcgcgtga tgcacaggtc agacaggagc
    46681 aactgcataa ggacaatgtg gcctttgcag aaaaactggt cgcagagggc cgtctggctc
    46741 cccgcgcctc ctccgtggtg gttgccctgc tggatgccgt cgccggtggc gacaagccgg
    46801 tggagtttgc tgagggggaa agccgcacac cgctggccac cgcctttcgt tcattgctct
    46861 ccgacgggga gccggtgatg aatttcgccg aacaggccac aaaagagcgt gtcggcgaca
    46921 cggtgaaggt ggatgtggca gagtttgcgg aagccgatcc tgagcgtctg gccctgcatc
    46981 agaaagcagt ggccctgtcc aaaaaagaag gcatcagcta tgaggctgct gtcgcacgct
    47041 gcctgtaatt taaggagaga gcatgtctga ttacttaaaa ggtaaacgtg tcgttgatcc
    47101 ggtactgacc agtatcgccc gtggctataa aaatgccgca ttcatcggcg aacgtatttt
    47161 ccccgtcgtg ctgacggaca aggaaggcgt gcgtgtaccg accttcggga aaaccgcctt
    47221 tgtggaatat gacaccgagc gtgccgtcgg ggcggacagc aatgttctgg tccgtgaaaa
    47281 aacaggcacg ctggacctgg tgctgggtga acacgatctg gctgcgccgg tggactatcg
    47341 cgagcaggcg gagtccatgt ttaacgaaga gagcaaggcc atccgtcgcg ccacgaatgg
    47401 cgtgaacctg cgccgtgaac ttatcgctgc ccgtctggct caggatgaaa aggtctaccg
    47461 taccgggcac gtcaaaaaac tgacagccag tgatcgctgg gccggtggta agggggaccc
    47521 catcggggtg attgaagccg gtatggaagc ggtccgtacg gccacggggc tgcgtcctaa
    47581 cctgatgacc atgggggccg gcgtgatggc gctgctgaag ttccacccgg cgattcaggc
    47641 cgccatcgga gccaacgaac gcaagcgcat caccacagaa atcctgcagg acctctttca
    47701 gatcgaagag atcgtcatcg gtgccccagt ctccctgccg tccatgaaag cggcaatgga
    47761 taagaacagc gtgccggcgg atatctgggg agacaatctg atgctgcact atgtcggcaa
    47821 accgcagccg ggggcggaca gcgcggacga gaacgagccg tccttcggct acaccctgcg
    47881 tcgtaagggg atgcctgttg ccgacaaata cgacggagcc ggtggcaagg tgaagtactg
    47941 ccgttatacc gatatctaca aagtcgccgt ggttggtggc gatgccgggt atctcattac
    48001 cggtatcagt aaataaggag gcgttatggg aaccactcag caggtcattc tgatcacaac
    48061 cgtaacggca ggggcagcac tggcacagca gcgttttgtc ggggcagata ataccccctg
    48121 taaagccggt gccgcagcgc tcggggttgc cgaagtggat gctgttaccg gcgacagcac
    48181 gccggtgagc gttctgggca ttattgctgt cgaggccggg gccgctgtca gccgtggtgt
    48241 ggctgttcag tcagatgctc aggccagagc cgtgccgcag tccggcgacg gtaaatcctg
    48301 tggtattgca cttgatgaag ccgggggtga aggcgacgtc attcgtatcc tgcgcggggt
    48361 gtgacatgta ctgcaccctg gaggatttgc ttgcgcaggt gccggagcgg acgcttatcg
    48421 agctcaccag tgaagagatg gacttcgact cacctgcaac agtgaatacc cgtgtggtgg
    48481 acagctgtat ccgctatgcc gacgagctga ttgatgccca tctgcgcgga cgctatatcc
    48541 tgccactggc agagataccg actgttctgc gggacattgc catcacgctg gtccgttacc
    48601 ggctctacgc ccgccgcccg gaaggtgacc tcccggatac ggtgaaggat gaccacaaag
    48661 aagcgctgcg gcaactcagg gagttacgtg ataacaggct cacgctgggg ctgccgtcca
    48721 ctcagaaaga tgtgcctgag cctggcgagt ttcgtgtacg cagtcgcccg gccactttcg
    48781 gcggtcgtga cggtttactg gagaaatact gatgaacgtt ctgcccgtcc ttgatgcggt
    48841 actggcccgg ttacgcgaga agctgccgca actgcaggtg gagtacttcc cggagaaacc
    48901 ggctgaatat cgcctgaacc atccggttgg cgcgttgctg ttgagctatg ccggttcgcg
    48961 ctttgacagg ccggatgata ccggtgcggt gatccagtct cagactatcc agctctgcgt
    49021 cacggtggtc ttccgccagc tcaacggtaa aaaaggggcg attaatgtcc tggatgctgt
    49081 ccgccgcatt ctcggtggcc acaccccgcc cggctgccgc cgccgtatct ggctgacccg
    49141 cgaggtgttt atcggtgaag tcagggggct gtggcagtac gccctcgact tcgcgactga
    49201 aagcgtcttt atcgaagaca gcgatttacc gtccggcccg ctgttaaccg aagtgaacta
    49261 tgaggaaagc gagtgatgaa agaataccgc tattccggcc cggccagcgg cgtcacgctg
    49321 tcggacggaa ccgaaatcct gctctggccg gggaagacgg tttccctgcc ggaggagcat
    49381 gactacgtga aggtactggt ggcgctgaaa catctgacgc cggtacctga agagactaaa
    49441 cccgccggca caccggctgt gcagtcacca aagcgcagaa gcagcagtga cagcgaagtg
    49501 aaaacggagg acacccatgg cagctaacta tctgcatggt cccgaaacca ttgaggtgga
    49561 aaacggtgcc cgcccggtta aaacggtgaa atctgccgtt attggcctga ttggtaccgc
    49621 cccgatgggg gatgtcaata cgctggtaca gtgcctgtct gagaaagacg cagcggcatt
    49681 tggcagccag ttcaccggct ttaccattcc gcaggcgctg gatgcgattt atgaccatgg
    49741 tgcaggcacc gttctggtca ttaacgtcct cgacccggcg aaacataaaa cggcgatcga
    49801 ggatgaggtg gttacctttg acaaatcgac agggcaggcc agactggcgc atccggttgt
    49861 cgctaatgtg gtggtgaaaa acagtgaagg cagcaccacc cacacggcga acacggacta
    49921 ccgtgttgat gcgcaggcgg gtgtgctcac gaacctgggc aaggctattg aggccggtgg
    49981 cagcgtgaag gtgagctatg agtacgcgga cccgtcgaag gtgactgcgg cggacatcat
    50041 cggcggggtg aacagcgccg gaaaccgaac cggcatgaag ctgcttaacg acagcttcaa
    50101 cctgtacggc tatttcgcca aaattctgat tgcgccggtg ttctgcaccc agaagagtgt
    50161 cgcagttgag cttatcgcca tggcagagaa gctgggcgcg gtaacctaca ttgatgcgcc
    50221 tgtcggtacc acctttgcac aggctctggc aggtcgtggc ccggaaggca ccatcaactt
    50281 caataccagc tccgaccgcg tccgtctgtg ctatccgcat gtgaaggtat atgacccggt
    50341 gacaaacaca gagcgtctgg agccgctcag ccagcgtgca gcaggtctgc gtgccagagt
    50401 cgatctggac aagggctact ggtggtcatc ctccaatcag gagattctgg ggatcaccgg
    50461 cgtggagcgc cagctgtccg cgatgattga tgacccgcag agtgaggtga acctgcttaa
    50521 cgaacagggg atcaccacgg tattcagcag ttacggcagc ggccttcgtc tgtggggtaa
    50581 ccggacggca gcatggccaa cggtcaccca tatgcgtaac tttgagaacg ttcgccgcac
    50641 cggtgatgtg atcaacgagt ccattcgtta tttcagccag cagtacatcg acatgccgat
    50701 tactcaggcg ctgattgatg cactgacgga gtcggtcaac gcctacggtc gcaaaatgac
    50761 tggtgatggt gcggtactgg gcttccgttg ctggtttgat ccggcccgca atccggagac
    50821 ggagctggcc gccgggcacc tgttgctgag ctacaaatat acgccaccac cgccgctgga
    50881 gcgactgacg tttgagactg agatcacctc ggaatacctg ttaaccctga aagggggcaa
    50941 ctgatgtcaa agattgagat aaaccgcatc acgaatgcca acatctatct ggatggtact
    51001 aacctgctgg gacgggctga ggaagttaaa ctccccgatg tctccatgat tatgcaggaa
    51061 cacaaggcgc tggggatggt gggtaaggtg gaactcccgg ctggttttga caaactggaa
    51121 ggcgaaatca aatggaacag cttttaccgc gatgcgatgc tgtctgccgc gaacccgtac
    51181 aggtcgctgg cactgcagtg tcgttccagc gtccagcgct acagctcgca ggggctgatt
    51241 gacgaaatcc cgctggtcac cttcctgacg attatgttca agaagaaccc gctggggacg
    51301 ttcaaacagc acgagaacgc cgagttctcc agtagcttca cctgcacgta tatcagacag
    51361 gtactggatg gtgaagagct gctgcaactg gactatctgg ccaacatctt ccgggtcggc
    51421 ggtgttgatc aactgactga ctaccgtatc aatatcgggg gctgacggtg agtgttgaac
    51481 tgacggataa aggaggacga tgtgcggcac tgggcatgtc aaatggtacg tggtttaccc
    51541 tccttgatat tccgggggtg gaaacccttt ttaatacccg taaaaccaat gacccgattg
    51601 actgcacacg ttcaaaggcc cgcaaactgg cggatttgat tgaagcatgg gagcctcccg
    51661 accactggtt ctccggcatc ggcaaatctg agggaaagac gcttctcatc gctttcctgc
    51721 gtaactgcaa ggggtttcgc acttgctgac atcacagggg ctccggcccc ttcttcttaa
    51781 tctcctttaa tatccgtcac gcgcttctcc cgacatactg ccctgaactt acacaggagc
    51841 acaatcatgt cacagacccc atccgatact tttaaattgt cttatccctt caccactgct
    51901 gcaggcacca gaattgagct ggttgaactg aaacgcctga cggtaaaaga cctgaagcag
    51961 gtgcgcaaaa tcagcaaaaa cccggcagac tgggacgaac cgctgattgc ccgcagtact
    52021 ggtcttctcc cggaggatct cgacaatatg gatctggctg attacctgca gttacagaaa
    52081 cgatttcagc tcatcacggg gatgggtgag agcaaccagg gcgctgacgc aggcgcaggg
    52141 gctgctggcg agatggttcc ggtttcagcc gggggagatt gatgccctcg atactgacga
    52201 tctggagatg tggctggagc aggctgaaga gcaaatcaaa agcgagtacg gcgacaaatc
    52261 atagtacaga cagccgccag tagcggctgt tctgcgttat cccctcacgt cttttcacct
    52321 tccccggagg ttaaccacta tgtcgggaca gttttcagtc ggcgttgtta tcggcgggat
    52381 gattggcagc acattccgtt ctgcaatgag cggtacccgc cgtgcgcttg attccctgag
    52441 cgatacctca cgccgcctgc aggaacgtca gaacgcttta acccgtgcaa cagaacgtta
    52501 tggtcaactg ggttcttccc ggatgcagca tctcaacagc gagctgctgc gggtaagccg
    52561 caccatggag caaattgagc gccagcagcg ccgtctgtca gcggcatccg ctaccagtga
    52621 tgcgctgaaa gctaaccgca tggcgctgta tggtcagggg attgaagcgt atggcatggc
    52681 acagactgtt tatcatacgg tttcccctgc cgttcagcag tccatgtctt ttcaggacaa
    52741 aatgattgat atgtcgatca ccgcaaaata tgacaataaa acgcgggatg cacttgccgg
    52801 acagataaaa ggctgggcgc ttaaatacaa tcagtatcag gatgagctgc aggaggcgat
    52861 gggttcactc atcagcgaca atattgataa tgtgtcagat atcggttttc tgatgccgga
    52921 tattgcccgc gcggcaacgg caacacgcac gtctgctcag gactgggcaa aagtggccgc
    52981 agtctggcaa aactccctga aaggcgcggc cagagatttt ggtgccgttc agaatattat
    53041 ggcttatgcc ggtgaccagg ggtcatttga aatcccggat caggtcaagt ggatgcagtc
    53101 cctggcccca atgatggcgg gtattgccag tggaaaagag gctgttgctg aaatcggggc
    53161 cagtctccag atagcaaaaa tcggtgcagg ttccaccgac gaagcagcca ataattttaa
    53221 aaactttctt accaaaattt ttgcccgcga tactcagaaa cagtttgctg atctgggtat
    53281 tgatttgcag ggatctattg cgagttataa agctgcgggg atctctccga ttgaagggat
    53341 gttgagtgtt atagaacgtt acctcaatgc caaaagcccc gaagcgctgg ccggcttcaa
    53401 atcagccatg aaaataaaga atgatacggc aagagatgag gcacttcagg ctctggcgaa
    53461 aaactttggt ctgggcgata tgttcgcgga tatgcaggtc atggcattta tccgcccgat
    53521 gctggccaac atggacagat atcgagagat ccgtgccggt gctctcaggg ctgcggataa
    53581 cgatttgctt gccagtgctt atgatcagcg gctgaaatct ccccttgaag ccactaaaac
    53641 acttatggtc agcagtcgcg atctggcaat tacgctgggc gatcaattag ctccatcttt
    53701 tatttctctg actcaggact tgctcccact cattcagggg acaaaacact gggtggcgac
    53761 tcacccgcaa tttgtcagtg gggcttttaa gctcatcagt gcgctccttg cgattaagat
    53821 agcgactgtt ggtctcaaac tggggctgaa tctccttatt tccccctttg tgaacgtctg
    53881 gaaaactgct gttttactcc ggaccaactg gcatcgactg actaccgcac tcggagaagg
    53941 tggcaaatta cgctggcttg tcactggatt cagccgactg accagcggag gactgaaact
    54001 cagcaaagtt ctggcgggca gcctcgttcg cggttttatg agcgctgcac gtgccgttct
    54061 ctggattggg cgagcgctga tgatgaatcc catcggtctc gttatcaccg ctgtcgcggc
    54121 agcagcttac cttatctacc gcaactgggg ggcagtcagt ggctggttta aacagcgctg
    54181 ggctgacatt caggaagcct ttaacggcgg tatcgcggga actggtaagc tgctgattaa
    54241 ctggtcgccg gcaggcctgc tctataaagc ctttgcggct gcgctgaaat atttcggcgt
    54301 tactctgccg gcaaagttca ccgacttcgg tggccatctt atcgacggtt tgattaacgg
    54361 tatcaaaaac aaatgggggt cgctcaaatc cagtgtaacc ggaatgggtg acagcatcag
    54421 tagctggttt aaacaatgct gggctgacat tcaggaagcc tttaacggcg gtatcgcggg
    54481 aactggtaag ctgctgatta actggtcgcc ggcaggtctg ctctataaag cctttgcagc
    54541 tgcgctgaaa tatcttggtg ttgatctgcc ggcaaagttt accgacttcg gtggccatct
    54601 tgtcgatggt ctgattaacg gtatcaaaaa caaatgggag tcgctcaaat ccagtgtaac
    54661 cggaatgggc gacagcatca gtggctggtt cagcgaaaag ctgggcattc attcgccgag
    54721 ccgcgtgttt atgggctttg gtgacaatat cgcgcagggg gccgccattg gcctgcagcg
    54781 gaccactccg cttgcagctc tggccgggca gcgaatggcc agtgaactgc tccccagaat
    54841 gcccgtgagc attcaggggc cagaaatacg ggataacact tcaggtgttc gcttcagtat
    54901 gccgttgccc gatatcaatg ggtttatgtc gtctgctaaa aatgcgatcg gagccgtaat
    54961 caatagtttg tcttccatgc ccgctattcc gctctccacc cgggcatcga ttttaccagg
    55021 gcaacgtctg gcaaatgaga tgacaccgga tgttccccgt atcccctcgc ctgaaatcct
    55081 ggctgccgga tattcaggcc gtggtgcagc tgcaaccggc ggtggaacgt ctggtggtat
    55141 ccaggtcagc tttaatcctc agtttttcct caatggcagg gaaaccacag cgcctgcagg
    55201 actgactggt gccctgaata tgagtctgca tgagctggaa aaaatgctgg agcgtctgct
    55261 ggctcagaaa caacgtaagg agtaccgctg atgtttgccg tactgggtga tattgagttt
    55321 gagctgatta cctactggga cggcttcgag gccacgttcg gcgtcgatta tgcggagcat
    55381 gcccgcatcg ggggtaagcc tggtctgcag ttcgtcggcg acaggctgga cgaaatccag
    55441 ataactctgg ttttccatca gcattattgt gtacccgatg tggagctggc gagactgcga
    55501 acagccatga aagcccatca ggcactggcg ctggtcttcg gcaacggtga ctatcgcggc
    55561 tggttcgtga tcaccgatgt gactgcaacc agcgagcaga ctgacagtac cggcaacgtg
    55621 ctggctgtca gtgccaccgt gtctctccgg gaatacaccg gtgacccgaa aaatcctctg
    55681 caaccaccgg caatacgcac gaagctcccg ggtgtcgggg cggtctccgg tgccatacct
    55741 tcaccttcag gggtggcgca gttcatccgc aacggcgtca actatgcgaa acaggcgcag
    55801 tctgtactcc agaccactat cagcgccgtt cgggtgacac agaaaatgaa ggataacccc
    55861 gttgtcgcac tgacccgtgt gccggggctg atgagcggac tgggtaatat ctccggggct
    55921 ctggggaaaa gtgttccggc gtttaacgca ctctctgaat ccatgcccga tgccatcagt
    55981 ctggccagaa cagccagcga agcagccacg tatgtacagc aggcacagtc tgcgctgagt
    56041 ggtgtggaca aaagaaatat tgcaggtgct ctggataccg tttccgggca gcttaacgcc
    56101 gtcggcacag cattcaaccg catgtctccg ggattaagtg caatggccgc cagaatactg
    56161 acgaggagtg tgtgatgttt cttgaacatg ttacccgtga cggagagcgc tgggattcgc
    56221 tggcatggca gtactacggt gacccgctgg gctatccccg gattattgcc gccaatccgc
    56281 acgtggccat cacgccggtg ctgccctccg ggctgttgtt actgatcccg gtgattgagg
    56341 ctgaagaagc ccgtacagaa gaggatattg ccccatggct gagataaaca gcactgcgca
    56401 agtcacatca gcgttaaccg gcgtcagcga tgtgctgaca ccggtgttca ctctgtggta
    56461 tctgcagaaa aacatcacct ctgatatcgc gccttatgtc acccgtgtga cctggagcga
    56521 taacatcaaa aatgagtccg ataccattga ggtggagctg gacgacaccg atggccgctg
    56581 gctggataaa tggtatccgg gcaagggtga cacgctgacg ctgaaaatgg gctatcaggg
    56641 cgagaagctg ctgtcctgcg gtacgttctc tatagacgag atcgaagtga gttcgcccgc
    56701 ttccgttgtt tctatccgtg gggtggccac ctcggttaac agtgccctgc ggactaaaac
    56761 cagtcgtggt tttgagaaca ccacgctggc agctgttgcg gggcggattg ccagaaagca
    56821 ccggctgaaa ctggtgggca gcattgagtc catcagaatc gaccgggtga cccagtatgc
    56881 tgaaaccgac gtgggttttc tgcgccggct ggccagcgag tatggttatg cagtgaaagt
    56941 ggtcagtgac cagctgattt tttctcatct ggccacactg cgcagtcagg agccggtcag
    57001 gcagttaaaa ccgcaggatg tggcccgctt ttccctgcgt gacaccatca accgggtcta
    57061 taaatctgca aaggtaaaac accagaaaag cagcagtaaa aaactgatcg tctacgaagc
    57121 tgatggtggt acccgtgaaa gcgacaaaaa gctcaaaggt ggtaaggtta ccagcgctga
    57181 ctcacttaaa gttaacagcc gcgtcagcga cccggacagt gcccggatta aagcggattc
    57241 agcactggcc agacataacg aataccagca gaacggctcc ctgacgctga cgggaacacc
    57301 tcaactgaca gcaggcaaca aaattgaact ggtgagtttt gggcagttat ccgggccatg
    57361 gctcataacc actgcccgcc atgcgtttga ccgtaacagc ggctacacca cagagctgga
    57421 agtggcacgg gggccagtca caagagggaa aaaacaaaaa actcagaaac tcacggttta
    57481 tcacccggat ggcagtacat cgacggtgat taaggagaag aaaaaatgac tggtgtcact
    57541 cgtcaggtcg gtacggtcag tgccgttgat gccgacaggg ttcaggcccg cgttcgtctg
    57601 cctgaatgcg ataacctgcg cacaaactgg cttaacgtgc tgcagcgcaa tacccaggat
    57661 aacaaagatt actggctccc tgacgtgggg gagcaggttg aggtgctgct cgatgccaac
    57721 ggcgaggatg gtgttattct gggcgcggtg tattcagacg tcgataaacc accgttcagt
    57781 gacaaaaata tccggggaac ccggtttgct gatggtgcag agtacagcta caaccggaag
    57841 acgcacactc tgatcatccg gggcggcatt gagcatattg tcattgagtg tggtgctgat
    57901 gtggtattga aaacacagaa agccacgatt gacgcaccgg aaaccgaact taccggagat
    57961 ctgcgtgtca ggggtaagct gatttacgaa ggaggcatgg cgggttctgg tggtgaaggt
    58021 gttaccgcga ccatccatgg caatatcgag attaaaggga atgtccatgc cacgggcagt
    58081 atgttgtctg atggcgaaaa ctccagccac cactcccact gagcttttta aacgccttta
    58141 atatcagcgt tcccgcacgg gggcaatact gcccccatga aaacaacctc agtattctgg
    58201 caaccagccc tgcaggctcc cggcgaaatt gtccgggggc tggatgatat ccggcaggcg
    58261 attcaaatta tcctgcggac tccccgcggc agcgacccgc atcgcccgga gttcggcagc
    58321 aatctgcatc tttatatcga ctggcctgta gaccgggcca ttccgcatgt ggtgcgcgaa
    58381 tccgtcgatg ccatccggcg ctgggagccc cgctgccagc ttatgtcagt taaacccgcc
    58441 gtcgacggcg aacatcttac gctccgggtg agctggaaag gctcagacgg acagacccgg
    58501 actcaggagc tgctatggcg ctgacagaac ccgattttat tgaacgcgat gccgacaaaa
    58561 tcacggcaga aatgattgcg aagtatgaag cggataccgg caaaacgctg tacccggcac
    58621 aggcagaacg tctgctgatt gatctgtggg cctatcgcga aatgctggtc agggttgcgg
    58681 tacaggaggc agcaaagcag aatctggtcg cctttgcccg tgagccgatg attgattacc
    58741 tcggtgaact ggtcggtgta taccgtcttg ccgcgcagcc tgccaccacc acgctccagt
    58801 tctccgtaga tgaggcactg gccattgatg tgctgatccc ggcaggcacc cgcgtcagcg
    58861 cttccgacag cgttattttt gccaccgata cagatgtggt actgaaggcc ggattgctgc
    58921 tggtcaatgt cacggccacc tgtaccgaac ccggtaccgc tggtaacggc tggcaacctg
    58981 cgcaggtcag tcagttactc gatgagattg ataacgttaa cctgctggtg agcaatctga
    59041 tggccagttc cggcggttca gaacaggaag acgatgacag gctccgggag cgtatcaggc
    59101 tggccccgga gtcattcacc aatgccggaa gccgtggcgc ataccgcttt catgccatgc
    59161 aggcccatcc caacattgtc gatgttgctg tgctttcccc ggttcccggg accgtagatc
    59221 tgtatccgct gctcagtacc ggtctgccgg acggcggtgt tctcacgctg gtagagagtt
    59281 tctgctctga tgagaaagtc aggccactca ctgatacagt gcgggctaaa acaccagtga
    59341 aggtggatta caccattgaa gccaggatta cgatctatcg tgatcaggat gccaggtctg
    59401 taaaagatgc cgctaacagc gccatacaga actgggtggc atcacgtgcc gccacgctgg
    59461 ggcgcgatat tgtccccagc cagattatca gtgcattgtc cgtttccggg gtgtaccagg
    59521 ttgaactggt gacaccggca ctgcgggtgg tggcagaaaa cgaatgggca aactgtacgg
    59581 caatcactct taacatgact ggagtgtctg atgactgagc cattacaact cccgccaccg
    59641 cttgagggtg atatcagtct caggacgctg ggaagactgg caggacggct ggataacatc
    59701 gacctgagcg tactgatggt ctttctcgtc gatatcgtcg acagttccgc gctgccatgg
    59761 ctgggcgagc agttctcact gtctggcgat ggctgggagc tggcggaatc ggacgatgtt
    59821 cgccgcatgc ttataaaagc agccatcgaa ctgcaccggt ataaagggac gccgtggtca
    59881 atccgggaag ttatccgccg ttttggcttt ggtgaagtgg atctgattga aggcacaggt
    59941 cgtctcagtt acgacggcaa tcgcagctat aacggactgt ttgttcatgg agatgcagcc
    60001 gcctgggcag tttatcgcgt tattctgaaa cagcccatta ctaacgatca ggccgcgatg
    60061 ctgcgtcaga cgctggctgc atttgcaccg gcccgctgcc atctggccag cctggagtat
    60121 cagtctgtgg ccattcgcta taacaatacc gccatccatg atggcagtta taaccacggg
    60181 agcagttgat tatgggaaac ctgaatgaaa cagaaaagtg ggaagaaaat atctatcaac
    60241 tggagacatc agatccggtt ctgggtggtg cagacgggat atcaaatcga gccccccggc
    60301 aactggcaaa caggacgaaa tggctgaaga agaaaacgga ggaagccgca cagtcactgg
    60361 ctgaacacgt acgttcccgt aaccacccgg acgcgacact gacagctaag gggttcaccc
    60421 aactgagcag tgccaccaac agcacctctg aaacgctggc cgccacacca aaagcggtca
    60481 aggctgcata cgacctggca gccggcaagg cgcctgccag ccacactcac ccgtggagtc
    60541 agataacggg agtgcctgcg gcttcactga cggcaaaagg caccgtacaa ctgagcagcg
    60601 ccacggacag tcaatcagaa actgaggctg ccacgccgaa ggcggtcaag gctgcatacg
    60661 accttgcagc cggcaaggcg cccgtcagcc acactcaccc gtggagtcag ataacgggag
    60721 tgcctgctgc ctcgctgacg gcaaaaggca ccgtacaatt gagcagtgcc atcaacagca
    60781 cgtctgaaat actggccgcc acaccgaaag cggtcaaggc tgcatacgat ctggcaaacg
    60841 gaaaacaacc ggcagatgcc acgctcactg ctctggcagg acttgccact gcagcagatc
    60901 ggttgcctta ttttaccgga gcagaccgcg cagagctggc aacccttaca gctattggtc
    60961 gcgctattat cgctaagggc agtataaaag atgtcctcaa ttaccttggt ttaggagaag
    61021 gctcggcgct acccgttggt gtgcctgttc cgtggcccac cgccacaccg ccagcaggct
    61081 ggttaaagtg cgacgggcgc gcctttacaa aagaacaata tcctgttctg gccagagtct
    61141 acccgaccct ccgtcttccc gatttacgcg gtgagtttat ccgtggatgg gacgacgggc
    61201 gcaaagttga tacaggacgt aagttgctgt ccgcacaagg ggcaacgctg ttaagaacag
    61261 caatgctgga ttattataac caggacacta cgggaacctc ggggatagtc ggcatgggat
    61321 tcaacaatga agattccatt acagaccttc gtgagggcag ctttaaaatg ccggacggga
    61381 caacattcag cgatcctgtc gtggcaatgt cagacaatgg tatgcaggct actattctga
    61441 cctctatcag aagcgggtat gcgaagggta tcactgtcag accccgtagc attgcactta
    61501 attacattgt gagggcagtt taatgagtaa cactgcagtt ctggatgaaa acggtatcgc
    61561 cactgtagcg ggcgatatca ctgtatatca ctatgacgag gaaacccggg aatacacctc
    61621 atcctctgtg gagtatctcg cccttggggt gggtactccg gcacattcgt gcgccgatgc
    61681 accgccggaa gcaatttcgg gttacgtggt ttgccggact gccacgctga acgggtggga
    61741 gcatgtgcct gatcaccgcg gcgagacggt atacagcacg gagaacggta accccgttct
    61801 gattacccaa ccgggtgatt acccggcgga caccaccaca aaacagccag ccacgccatg
    61861 ggatacctgg aacggtgagg cgtgggtaac cgatactgaa cggcagcgag ccgcagaact
    61921 ggaggttgcc agacagcaac gccagcaacg ggtgaaacag gcgatggcgt ccgtcgatct
    61981 tatcaacctc aaactgcgtg ctggtcgcag tctgaaacca gaagaaacgg caaaactgaa
    62041 cgccgtgctg gattatatcg acgagctgaa cgcactggat atcagcaagg cacctgaaat
    62101 cagctggccg gaagcgccac tggcgcttgc cggctgaacg gtatcacgcc gccctcacga
    62161 tatagttaaa tgcgatattg cgggggcgag ctgatacata agcccaccag tcatatcttt
    62221 gtgcaccttt agaggatgca tcaaatatcc actttttgcc atttaagacc cccagttgat
    62281 tagaggttaa agcgtcacca aatccgtatt gtgtagagcc gagcgaactg atatctccgg
    62341 tatcattatc atcaaaaccg gatacgatag tcccttcctg aaatgaaagt agcgcacgcc
    62401 cggtatcaac cttgcgacca ccgtcccatc cacggataaa ctcaccgcgc aaatcgggaa
    62461 gacggagggt cgggtagact ctgaccagaa caggatattg ttctttggtg aacgtagctc
    62521 cgttgcactg taaccagcct gctggcggtg tggcggtggg ccatggaacc ggtacaccaa
    62581 caggcaatgc agagccttcc cccaaaccaa cgtttctatg ccgcccttac gatgtaatta
    62641 aacgcaatgt tacggggacg aatggtcaca taattaaccc catcgcctcg cgaaggacct
    62701 gcaaatgaaa aacgggagaa tccaggttgg ttgtcaacga tttggtcata gttattgatt
    62761 gaatgaccag acccaatgtc aaaattacct gcgtaatgag aaaaacttgt accatcctgc
    62821 cgtgaaagca gatcgcgccc tgtatccact tttcgcccgt cgtcccatcc acggataaac
    62881 tcaccgcgta aatcgggaag acggagggtc gggtagactc tggccagaac aggatattgt
    62941 tcttttgtaa aggcgcgccc gtcgcacttt aaccagcctg ctggcggtgt ggcagtgggc
    63001 cacggaacag gtacaccaac gggcagcgcc gagccttccc ccaaaccaac gtttatctct
    63061 caaatcctcc ccacattatc tcagtacttt aaccactcaa aagggagtat ttttaatgct
    63121 gattggctac atacgtgtgt caacaaatga ccaaaacaca gatttgcaac gcaatgcatt
    63181 aatgtgcgca ggatgtgaac agatttttga ggacaaaatg agcggaacca agtcggaacg
    63241 accaggcctg aaacgcgctt taaagtgcct taaaagaggg gatacattgg tggtctggaa
    63301 gctggatcgg ttgggtagaa gaatgaaaca cctcattgct ctcacagaag agctacgcgc
    63361 aaaaggtgtc aattttcgca gtctgacgga ttcaatcgat accagcactc cgatgggaag
    63421 gttctttttt catatgatgg gtgcactggc agaaatggag cgtgaactga tagttgagcg
    63481 cacgctggct ggactggctg ctgcgcgagc acaaggaaga gttggaggac gtcgcccgaa
    63541 actgacgaag gagcagcatg agcagatcgc aagattgctc cagaaagggt atgacagaaa
    63601 gcggctggca attatctatg atattggact gtcaacgatc taccgctatc accctgttgg
    63661 gactgtcata acgcaacctg aaatgtaatt ctttttcaaa taatgaaacg ccgcgtggat
    63721 gccatttatc gcacaagata gagtgcattt atcgcgcggc gcatcattct tcatgtgaat
    63781 catccgttga ttaattatat ataaacatta tgtatcaaca agttacattg ataattaacg
    63841 ctaccattaa taaaaatacc ccacaaggtc cgaaaaaaaa cgccggcaat gtgccgacgt
    63901 ctttgaaaag aaaggattgt ctatgttatc gcccgttatt cattcagccg ggttgactta
    63961 tccgcgccaa ttgctcgaaa tagtctggaa aagttttagc ggtacatttt ggatcaagaa
    64021 tcgtcacagg cgtatctgat aatgcgacca gcgagaaaca catcgccatc cggtgatcat
    64081 tataagtggc gatctcagca aatttcagtt tttccggtgg cgtaatgcga atgaaatcat
    64141 gcccctcttc gacttccgca ccgactttac gcagttctgt tgccatcgca aacaggcgat
    64201 cggtctcttt aacacgccag ttatagatat tgcgcagcgt ggtggtgcct tttgcaaata
    64261 acgccgcggt ggcaatggtc atcgccgcat cgggaatatg gttcatatcc atatcaatgg
    64321 cgttcagttc accacgcgtg caggaaatat aatcatcgcc ccagcaaatg gtcgcgccca
    64381 ttttttccag aacatcagca aagcgaatat caccctgcat actgttgcgt ccaatgccgg
    64441 tcacttttac agtgccgcct ctgattgctg ctgctgccag gaaataagaa gccgaagatg
    64501 catcgccttc aaccaaataa gtgcccggag actggtaaga ctgcccccct tttacgacaa
    64561 attgttgata gtgctgattt tcaatttcaa caccaaacgt cttcatcaga ttgagggtga
    64621 tatcgatata aggtttagaa accagatcgc ctttaatacg aatcaccgta tcttccggcg
    64681 caagaggcgc agtcattaac agtgcggtga ggaactggct ggaaacggag ccatcaacat
    64741 caacgttgcc gccagtaaag ccgccctgta aacgcaatgg cggataattt tcttgttcca
    64801 ggtaagtgat cttcgccccg ccctggcgca gcgcatccac cagatgacca atcgggcgtt
    64861 ctttcattcg cggttcaccg ctcagtacaa tatcattgct acccaggcaa agtgctgccg
    64921 ccagcggacg cattgccgtt ccggcgttac cgaggaacaa ctcccgggca ctttctgcgt
    64981 gtaatggacc accgttaccg ataatttcgc aacgcgtacg atcggctgaa agcgtatagc
    65041 ttacccctaa cgctgttaat gcattcagca tatggcgcac gtcatcgcta tccagcagat
    65101 tggttaatac tgttttgccg tgtgctaatg ccgccagcaa taaagcgcgg ttagaaacgc
    65161 tcttggaacc gggcagatta atagtgccat cgacacgagc gatgggttgt aacgttaggg
    65221 attccatgaa ctcaactctc tacaacagaa ataaaaaccc cacaggctgg ctgtggggat
    65281 tatgcaaaat ttcgacatta gccgtgacga cgttcgaact caaccatgaa gtctgtcagt
    65341 gctttaacac cttccagcgg catggcgtta taaatagacg cacgcattcc gccgaccact
    65401 cggtgacctt tcagcgcatg taagcccgca gcgaaagact cttcgaggaa caatttatca
    65461 agcgcgctat ccgccaactg gaacggcacg ttcatccgcg aacggttagc tttcgccaca
    65521 tcattgcggt agaaatcgct gttatcaatc accccataca gcagttctgc tttttgctga
    65581 ttgatcttat ccattgcagc cacaccgccg ttcgctttca gccatttgaa gaccaggcca
    65641 gataagtacc aggcaaatgt cggcggcgtg ttgaacatgg aatcgttatc gttgaggatg
    65701 gagtaatcca gaatcgatgg gcaggcgata ttcgctttgc ccagcaaatc ttcacgaacg
    65761 atgacgattg tcaggccagc cgggccgata tttttctgcg cgccagcgta aattacacca
    65821 taacggctga catcaatcgg acgggaaaga atggttgaag agaagtcagc ggcgaccacc
    65881 acgtctttac cgaagtctgg tgtttcatcg atagcgatac catcgatggt ttcgttcggg
    65941 cagtaatgca tataagcagc attatcagag agttgccatt cactcattgg cttaactgcg
    66001 cgaagaccat caacggtcac tttcgcatca aacacatttg gtgtgcagta ttttttcgct
    66061 tctttgatag cacttgctgc ccagtaaccc gcatcaacat aatctgcagt ggttttatca
    66121 ccaagaatat tcaacggtac tgcagcaaat tgaccgcgac caccgccatg gcagaacaat
    66181 actttgtagt tggaggggac gttaagaaga tcgcgaaaat ccttctcggc ttcctctgca
    66241 acctgaatga actctttgcc acggtgactc acttccatca ccgacgtacc aagaccgttc
    66301 cagtcgcgca gttcctgttg agcctgttta agcacctctg ccggtagcat tgccggacca
    66361 gaactaaaat tgaagatttg agccatttcc cctcaccacg ttgcgttgct attaacccgc
    66421 ggtcaatcga ttgcgaccac aggtaataca ttattccagt ggctatcggt tttatcattc
    66481 agtgacacac atcgcaatga gtaaaaccgt tgagggcaaa aatgtggctt gcatcacaca
    66541 aaagaatctc tcggattatt agcaaaaagc cgatgttttt gctgtcaggt cacccgttta
    66601 gctcgacttg ccttcgtcca ttctggattt gcacagcgcg gacataattg tttttccccc
    66661 gcgttcatat gaatgatttc actgcaacgg atgcaggcat agtccgccga ctccggcacc
    66721 actttgtaac gctgagtaca gtcactgggt aaaataagta accccggttt caccttgtca
    66781 tcttcaaaga aataaacact gatttgcccg ttggtttcga gaatcgccag acgtacctgc
    66841 cccaactgct ctacaccgcg caatcgtagc tccataaaga actcaaattc cgtcatgttg
    66901 gagttattga gtttcgacca ggccagctcg ccgtcttcaa taatgacaac cggcttgcct
    66961 tccagaagat cttccagttt ttcactgtgc gccatcaacc acattaccag gcggtataac
    67021 aacgccagag taataaagac gataagtacc gggaccatcg gcacatcatc ataaaacgcc
    67081 acatctcccg ccgctgaacc cagtgtcaga atgattaaca cttcaaacag cgacatctgc
    67141 cgcacaccgc gtcttccggt cattttgagg aacaaaaaaa ccagtacaaa ggtataaaga
    67201 ctacgtagtg caacttcgcc aaggaaatca aaaggcactt tatcaaatgc catacggtgg
    67261 agatcgaacg ccttcatttt tatgctctta tcgtggttta tccgctaaaa gcatagccaa
    67321 cagttcatcg gcatggcggc ggtgatagcg cctttgagat aaaacccgta ttattgcgcg
    67381 ctttccgtac gactaaagtg attttcatga cgcaaacatt tatccccggc aaagatgccg
    67441 ctctggaaga ttccatcgct cgcttccagc aaaaactttc agacctcggt tttcagattg
    67501 aagaggcctc ctggctgaat cccgtgccta atgtctggtc tgtacatatt cgcgacaaag
    67561 agtgcgcact gtgttttacc aacggtaaag gcgcaaccaa aaaagcggcg ctggcttctg
    67621 ccctcggtga atatttcgag cgtctctcaa ccaactactt ttttgcggac ttctggctgg
    67681 gcgaaaccat cgccaacggc ccattcgtgc attatcccaa cgaaaaatgg ttcccactga
    67741 ccgaaaatga cgatgtacca gaagggctgc tcgatgaccg tctgcgcgca ttttacgatc
    67801 cggagaatga actgaccggt agtatgctga ttgacctgca atccggtaac gaagatcgtg
    67861 gtatttgcgg tctaccgttt acgcgccagt ccgacaatca gaccatttat attccgatga
    67921 atatcattgg taacttgtac gtttctaacg gtatgtctgc tggcaatacc cgtaacgaag
    67981 cacgcgttca ggggttatcc gaagttttcg aacgttacgt gaaaaaccgc attattgctg
    68041 aaagcatcag cttgccggaa atcccggcag acgtgctggc acgttatcca gcagtagttg
    68101 aagcgatcga aacgctggaa gcggaaggtt tcccgatctt cgcttatgac ggttcgcttg
    68161 gcggccagta tccggtgatt tgcgtggtac tgttcaatcc tgctaacggt acctgctttg
    68221 cctcattcgg tgcgcatcct gattttggcg ttgcactgga acgtaccgtg accgagttgc
    68281 tgcaaggtcg tggcctgaaa gatctggatg tgtttactcc accaaccttc gatgatgaag
    68341 aagtcgctga acataccaac ctcgaaacgc actttatcga ttccagcggt ttaatctcct
    68401 gggacctgtt caagcaggat gccgattatc cgtttgttga ctggagcttc tccggcacca
    68461 cggaagaaga gttcgccacg ctgatggcta tcttcaaaaa ggaagataaa gaagtttata
    68521 ttgccgatta cgagcatctg ggtgtctact cttgccgtat tatcgtacct ggcatgtccg
    68581 atatttatcc ggctgaagat ctgtggttgg cgaataacag tatgggcagc catttacgtg
    68641 aaacgattct gtcactgccc ggtagcgagt gggaaaagga agattacctg aacctcatcg
    68701 agcaactgga tgaagaaggt tttgatgact ttacccgcgt gcgtgaactg ttgggcctgg
    68761 cgaccgggtc ggataacggc tggtacactc tgcgtatcgg tgaattaaaa gccatgctgg
    68821 cgctggctgg tggcgatctg gaacaggctc tggtctggac cgaatggacg atggagttta
    68881 actcatcagt attcagcccg gaacgcgcca attattatcg ctgcctgcaa acattgttat
    68941 tactggcgca ggaagaagat cgccagccgc tgcaatatct gaatgcgttt gttcgcatgt
    69001 acggcgcaga tgccgtggaa gccgccagtg cagcaatgag cggcgaagcg gcgttttatg
    69061 gcttgcaacc agtagatagc gatctgcacg cgtttgctgc acatcagtcg ttgttgaagg
    69121 cctacgaaaa gctgcaacgc gccaaagcag cattctgggc aaaataaaat caaatagcct
    69181 acgcaatgta ggcttaatga ttcgtctgag ttatattacg gggcgttttt ttaa
//
LOCUS       DACADF010000005        66028 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.005, whole genome shotgun sequence.
ACCESSION   DACADF010000005 DACADF010000000
VERSION     DACADF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.005"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(166..801)
                     /gene="nth"
                     /locus_tag="ISB30_000330"
     CDS             complement(166..801)
                     /gene="nth"
                     /locus_tag="ISB30_000330"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP1988015.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAYGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(801..1496)
                     /locus_tag="ISB30_000331"
     CDS             complement(801..1496)
                     /locus_tag="ISB30_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAP1988016.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(1500..2120)
                     /gene="rsxG"
                     /locus_tag="ISB30_000332"
     CDS             complement(1500..2120)
                     /gene="rsxG"
                     /locus_tag="ISB30_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAP1988017.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(2124..3182)
                     /gene="rsxD"
                     /locus_tag="ISB30_000333"
     CDS             complement(2124..3182)
                     /gene="rsxD"
                     /locus_tag="ISB30_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAP1988018.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMMLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTTSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGILAGA
                     GWQWVNLAWLTGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(3183..5603)
                     /gene="rsxC"
                     /locus_tag="ISB30_000334"
     CDS             complement(3183..5603)
                     /gene="rsxC"
                     /locus_tag="ISB30_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAP1988019.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKTARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQQANAEPEEQVD
                     PRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQQANA
                     EPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAA
                     QQKVVNED"
     gene            complement(5596..6174)
                     /gene="rsxB"
                     /locus_tag="ISB30_000335"
     CDS             complement(5596..6174)
                     /gene="rsxB"
                     /locus_tag="ISB30_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAP1988020.1"
                     /translation="MNAIWIAVAAVSLLALAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(6174..6755)
                     /gene="rsxA"
                     /locus_tag="ISB30_000336"
     CDS             complement(6174..6755)
                     /gene="rsxA"
                     /locus_tag="ISB30_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAP1988021.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(6832..7272)
                     /locus_tag="ISB30_000337"
     CDS             complement(6832..7272)
                     /locus_tag="ISB30_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAP1988022.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(7358..7573)
                     /gene="ydgT"
                     /locus_tag="ISB30_000338"
     CDS             complement(7358..7573)
                     /gene="ydgT"
                     /locus_tag="ISB30_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAP1988023.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(7845..7970)
                     /gene="blr"
                     /locus_tag="ISB30_000339"
     CDS             complement(7845..7970)
                     /gene="blr"
                     /locus_tag="ISB30_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAP1988024.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            8213..9253
                     /locus_tag="ISB30_000340"
     CDS             8213..9253
                     /locus_tag="ISB30_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP1988025.1"
                     /translation="MSDNIRVGVIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKTLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(9289..10290)
                     /gene="add"
                     /locus_tag="ISB30_000341"
     CDS             complement(9289..10290)
                     /gene="add"
                     /locus_tag="ISB30_000341"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP1988026.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLT
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10394..11566)
                     /gene="malY"
                     /locus_tag="ISB30_000342"
     CDS             complement(10394..11566)
                     /gene="malY"
                     /locus_tag="ISB30_000342"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAP1988027.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKIAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            complement(11576..13168)
                     /locus_tag="ISB30_000343"
     CDS             complement(11576..13168)
                     /locus_tag="ISB30_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAP1988028.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTMDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMVGLMRTVQA"
     gene            13343..14371
                     /locus_tag="ISB30_000344"
     CDS             13343..14371
                     /locus_tag="ISB30_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAP1988029.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVIWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            14483..15250
                     /gene="hdhA"
                     /locus_tag="ISB30_000345"
     CDS             14483..15250
                     /gene="hdhA"
                     /locus_tag="ISB30_000345"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAP1988030.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLIAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTNALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGDGVQELN"
     gene            15479..16069
                     /locus_tag="ISB30_000346"
     CDS             15479..16069
                     /locus_tag="ISB30_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1988031.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            16459..18270
                     /gene="uidA"
                     /locus_tag="ISB30_000347"
     CDS             16459..18270
                     /gene="uidA"
                     /locus_tag="ISB30_000347"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP1988032.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQHWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            18267..19639
                     /gene="uidB"
                     /locus_tag="ISB30_000348"
                     /pseudo
     CDS             18267..19639
                     /gene="uidB"
                     /locus_tag="ISB30_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            19678..20944
                     /gene="uidC"
                     /locus_tag="ISB30_000349"
                     /pseudo
     CDS             19678..20944
                     /gene="uidC"
                     /locus_tag="ISB30_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(20989..22497)
                     /locus_tag="ISB30_000350"
     CDS             complement(20989..22497)
                     /locus_tag="ISB30_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAP1988033.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPTLNVPAVPAIPQQ"
     gene            complement(22598..23773)
                     /gene="manA"
                     /locus_tag="ISB30_000351"
     CDS             complement(22598..23773)
                     /gene="manA"
                     /locus_tag="ISB30_000351"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAP1988034.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVHDAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANKLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            23972..25618
                     /locus_tag="ISB30_000352"
     CDS             23972..25618
                     /locus_tag="ISB30_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAP1988035.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILCDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            25761..27164
                     /gene="fumC"
                     /locus_tag="ISB30_000353"
     CDS             25761..27164
                     /gene="fumC"
                     /locus_tag="ISB30_000353"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAP1988036.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGG"
     gene            complement(27161..28090)
                     /gene="tus"
                     /locus_tag="ISB30_000354"
     CDS             complement(27161..28090)
                     /gene="tus"
                     /locus_tag="ISB30_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAP1988037.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSVARFEWVHRHFPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(28166..29467)
                     /gene="rstB"
                     /locus_tag="ISB30_000355"
     CDS             complement(28166..29467)
                     /gene="rstB"
                     /locus_tag="ISB30_000355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAP1988038.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(29471..30190)
                     /gene="rstA"
                     /locus_tag="ISB30_000356"
     CDS             complement(29471..30190)
                     /gene="rstA"
                     /locus_tag="ISB30_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAP1988039.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVSLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            30319..30654
                     /locus_tag="ISB30_000357"
     CDS             30319..30654
                     /locus_tag="ISB30_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAP1988040.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(30651..31373)
                     /gene="folM"
                     /locus_tag="ISB30_000358"
     CDS             complement(30651..31373)
                     /gene="folM"
                     /locus_tag="ISB30_000358"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAP1988041.1"
                     /translation="MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSLPLDGGRHLR"
     gene            complement(31410..32792)
                     /locus_tag="ISB30_000359"
     CDS             complement(31410..32792)
                     /locus_tag="ISB30_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP1988042.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(32978..33922)
                     /locus_tag="ISB30_000360"
     CDS             complement(32978..33922)
                     /locus_tag="ISB30_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1988043.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            34446..35978
                     /gene="pntA"
                     /locus_tag="ISB30_000361"
     CDS             34446..35978
                     /gene="pntA"
                     /locus_tag="ISB30_000361"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAP1988044.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQTAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            35989..37377
                     /gene="pntB"
                     /locus_tag="ISB30_000362"
     CDS             35989..37377
                     /gene="pntB"
                     /locus_tag="ISB30_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAP1988045.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALIVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(37402..38436)
                     /gene="tqsA"
                     /locus_tag="ISB30_000363"
     CDS             complement(37402..38436)
                     /gene="tqsA"
                     /locus_tag="ISB30_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAP1988046.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            38848..39213
                     /gene="mdtJ"
                     /locus_tag="ISB30_000364"
     CDS             38848..39213
                     /gene="mdtJ"
                     /locus_tag="ISB30_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAP1988047.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            39200..39529
                     /gene="mdtI"
                     /locus_tag="ISB30_000365"
     CDS             39200..39529
                     /gene="mdtI"
                     /locus_tag="ISB30_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAP1988048.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(39568..40389)
                     /locus_tag="ISB30_000366"
     CDS             complement(39568..40389)
                     /locus_tag="ISB30_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP1988049.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(40489..40572)
                     /locus_tag="ISB30_000367"
     CDS             complement(40489..40572)
                     /locus_tag="ISB30_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988050.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(40665..41000)
                     /locus_tag="ISB30_000368"
     CDS             complement(40665..41000)
                     /locus_tag="ISB30_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAP1988051.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            complement(41397..42650)
                     /locus_tag="ISB30_000369"
     CDS             complement(41397..42650)
                     /locus_tag="ISB30_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1988052.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNHFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTARYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            42757..43650
                     /locus_tag="ISB30_000370"
     CDS             42757..43650
                     /locus_tag="ISB30_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1988053.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            43785..45005
                     /gene="mlc"
                     /locus_tag="ISB30_000371"
                     /gene_synonym="dgsA"
     CDS             43785..45005
                     /gene="mlc"
                     /locus_tag="ISB30_000371"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAP1988054.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            45130..45825
                     /gene="bioD"
                     /locus_tag="ISB30_000372"
     CDS             45130..45825
                     /gene="bioD"
                     /locus_tag="ISB30_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAP1988055.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(45778..47070)
                     /gene="clcB"
                     /locus_tag="ISB30_000373"
     CDS             complement(45778..47070)
                     /gene="clcB"
                     /locus_tag="ISB30_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAP1988056.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWLPDGEEITLLLGLTGMATLLAATTHAPTMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(47231..47845)
                     /gene="dmsD"
                     /locus_tag="ISB30_000374"
     CDS             complement(47231..47845)
                     /gene="dmsD"
                     /locus_tag="ISB30_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAP1988057.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTNDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDNFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(47888..48742)
                     /locus_tag="ISB30_000375"
     CDS             complement(47888..48742)
                     /locus_tag="ISB30_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAP1988058.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHII
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGIIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISILALIACAGLIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(48744..49361)
                     /gene="dmsB"
                     /locus_tag="ISB30_000376"
     CDS             complement(48744..49361)
                     /gene="dmsB"
                     /locus_tag="ISB30_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAP1988059.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(49372..50183)
                     /locus_tag="ISB30_000377"
                     /pseudo
     CDS             complement(49372..50183)
                     /locus_tag="ISB30_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005022811.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(50228..50941)
                     /locus_tag="ISB30_000378"
                     /pseudo
     CDS             complement(50228..50941)
                     /locus_tag="ISB30_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432394.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(51002..53428)
                     /locus_tag="ISB30_000379"
     CDS             complement(51002..53428)
                     /locus_tag="ISB30_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HAP1988060.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDKYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(53627..53932)
                     /locus_tag="ISB30_000380"
     CDS             complement(53627..53932)
                     /locus_tag="ISB30_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAP1988061.1"
                     /translation="MKHSTYCAALLLALASPTVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            54040..54750
                     /locus_tag="ISB30_000381"
     CDS             54040..54750
                     /locus_tag="ISB30_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAP1988062.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTLVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(54753..55313)
                     /gene="speG"
                     /locus_tag="ISB30_000382"
     CDS             complement(54753..55313)
                     /gene="speG"
                     /locus_tag="ISB30_000382"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HAP1988063.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(55348..55689)
                     /locus_tag="ISB30_000383"
     CDS             complement(55348..55689)
                     /locus_tag="ISB30_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAP1988064.1"
                     /translation="MKITLSKRIGLLAFLLPCALVLSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            55824..56150
                     /locus_tag="ISB30_000384"
     CDS             55824..56150
                     /locus_tag="ISB30_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HAP1988065.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            56356..57570
                     /gene="rspA"
                     /locus_tag="ISB30_000385"
     CDS             56356..57570
                     /gene="rspA"
                     /locus_tag="ISB30_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAP1988066.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            57582..58601
                     /locus_tag="ISB30_000386"
     CDS             57582..58601
                     /locus_tag="ISB30_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HAP1988067.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFDEKGIQPTLVIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            58659..60026
                     /locus_tag="ISB30_000387"
     CDS             58659..60026
                     /locus_tag="ISB30_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAP1988068.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALQDAAEDQPHVVRAAQ
                     PSRSL"
     gene            60115..61575
                     /locus_tag="ISB30_000388"
     CDS             60115..61575
                     /locus_tag="ISB30_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP1988069.1"
                     /translation="MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGVE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYTLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPDNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(61611..61814)
                     /gene="ydfZ"
                     /locus_tag="ISB30_000389"
     CDS             complement(61611..61814)
                     /gene="ydfZ"
                     /locus_tag="ISB30_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAP1988070.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(61991..62677)
                     /locus_tag="ISB30_000390"
     CDS             complement(61991..62677)
                     /locus_tag="ISB30_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP1988071.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(62766..63512)
                     /gene="ydfG"
                     /locus_tag="ISB30_000391"
     CDS             complement(62766..63512)
                     /gene="ydfG"
                     /locus_tag="ISB30_000391"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAP1988072.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            63649..65694
                     /gene="dcp"
                     /locus_tag="ISB30_000392"
     CDS             63649..65694
                     /gene="dcp"
                     /locus_tag="ISB30_000392"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAP1988073.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNNWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWRRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGTAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            65843..65947
                     /locus_tag="ISB30_000393"
                     /pseudo
     CDS             65843..65947
                     /locus_tag="ISB30_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00156 family protein"
BASE COUNT        16595 a        16046 c        17006 g        16381 t
ORIGIN      
        1 tagaaaattt cgctattaaa aaaacaatta acctgcattt ttggttatct gcaaacggat
       61 tacctgatga taacgctaag caatggcatc attgacgtta aaaagatgtt ttaaaagagg
      121 ataaagaaag gttatgaatg gggtaatcgg tgttacccct gatctttaga tgtcaacttt
      181 ctctttgtat tcacaaagat cttcaataat acaagagcca cagcgaggct tgcgggcaat
      241 gcaggtataa cgcccgtgca ggatcaacca atggtggcag tcgactttaa actctgccgg
      301 aaccactttc agtagctttt cttctacctg ttcgacgttt ttccccggcg caaattgagt
      361 acgattgcaa acgcggaaaa tgtgcgtgtc gacagcaata gtcggccagc cgtatgcagt
      421 gtttaatacg acgttggctg ttttacgacc tacaccaggc agggcttcaa gcgcagcacg
      481 atcttccgga acctcgccat tatgttgttc cagtaatata cggcaggttt tgatgatatt
      541 ttctgctttg ctgttataaa ggccaatcgt tttgatatag gttttcaccc cttcaacgcc
      601 cagttcaagc atcgctgcag gcgtattagc caccgggtag agtttcgccg tcgccttatt
      661 aacactgaca tcggtcgcct gagcggaaag cagtacggca atcagcaatt caaaaggcga
      721 actgaaatta agctcggtgg tgggatgagg attgttctca cgcaggcgag tgaggatctc
      781 caggcgtttt gctttgttca tcagacattc cctgtttcac cgtttggcaa tgcacgttcg
      841 gcagctgctt cagtacggcg ctttttcatt ttttcatcaa tcaggtattt tcctgccagc
      901 atcagtccca ggccaataaa tgcacctggt ggcagcatcg ccagcaggaa aggggagtcg
      961 gtgtggaaaa tctccacgcg taatactttt gcccagctac ctaacagcgc atctgcaccg
     1021 tcaaacaacg tgccattgcc gataatttcg cgtagtgaac ccagcacgaa catggcgcag
     1081 gttgcgccca taccaattga aaagccgtcc agtgccgaaa gcgccggacc ttttttggcg
     1141 gcgaaggctt cagcgcggcc cacaacgata cagttagtga caatcagcgg aataaaaatc
     1201 cctaatgatt gatacaggcc aaaggcgtag gcgttgatca gcatctgtac agcgctgacc
     1261 accgaggcga tgatcatcac gtaaatggga atgcggatct cggcgggcgt ccagtgacgc
     1321 agcgtcgaaa tggtcaggtt ggttagcgtc agtaccagcg tagtcgcaag tcctaaaccc
     1381 agagcgttag tggcagtgga cgtgaccgcc aacagaggac aaaggccgag cagctggacc
     1441 agcgcagagt tgtttttcca caacccctga acaataacgt ctttaatttc gctcacgttt
     1501 tattctccac aggcaggaag ttgagaaagt tgtgccggta acgtctgagc gtacaatccg
     1561 gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg tcgcgccagt gaactggtcg
     1621 aaatcaccac catctttctt caccgcccag ttcgcatcat ccgcaccact gattttttta
     1681 cccgcaaaat gggtgatcca gtcagaaagg cgcagttcga ttttatcgcc aagccctggc
     1741 gtttcgtggt gctcagtcac gcgcgtgcca agtaccgtgc cgttaaaatc ggctcccacc
     1801 agcagctgaa tcgcaccgga atagccatct ggcgcggttg cttccagaac ggcagctacc
     1861 ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac ctttacctaa ctctggcgca
     1921 gttaccagat agcaactctg tgccagcgca ttgttatagc gttcagctgg cagcacctga
     1981 tcaaataacg ccttttgttg cagactggcc tgctcagcaa ttgtcgtttt ggtcatctgg
     2041 ttgatggccg cagttaaccc tgttgaaccc gctgcaaaga gcgccaacgt aatgccgtgt
     2101 tttcggatag ttttcagcat ggtttaccct ttgcgatggc cgtagacgcg cggacgcgtg
     2161 tagtaatcga tcagaggaac cgtgatgttc gccagcagga cggcaaaagc cacgccgtca
     2221 ggatagccgc cgaaactgcg gatcaaccag actaataagc ccgccagcgc gccgaaaata
     2281 agacgaccac gattggtcgt agaagcggta accgggtcag tcaaaataaa gaatgcgccg
     2341 agcatggtcg caccagacag cagatgaatt tgcggtgctg ccagagtatc tggtgagaac
     2401 aaccaaccca acgttgcgca taacgccagc gttactaaga agctgagggg aatatgccag
     2461 cgaatcgctt tctgccatag caaccacacg ccgccagtca gccaggcgag atttacccat
     2521 tgccaaccag cgcccgccag aataccgctg tagatcggat attgcataat ctcttcaacc
     2581 gaatgaccgg cacggacaga ggttttaaat gtatccagcg gtgtcgcctg actaatgcca
     2641 tcaataccta agcgtagcgt gttcatatca ccaccactgg tggtatgtcc gctgaaaata
     2701 acctgtatgg cgtcgataaa accagggatg ttgaccgcaa tttcatgtgg cggtaaccag
     2761 ctggtcatct gcaccgggaa ggagatcagt aagaccacat aaccaatcat tgccggatta
     2821 aacgggtttt gccccagacc gccatacaac tgtttagcga tgatcaccgc aaacaccgta
     2881 cccagcacga ccatccacca tggcgcgagg gggggaatac ttaccgccag caataagcct
     2941 gtcagcaatg ctgagttatc tttcaacgtt gcagctaccg actgcttgcg tagtttgagg
     3001 acgagagctt cggccagaag agcactgacc gacgccaaca ggatctgaac gagagtaccc
     3061 caaccaaaaa accacagttg cgctgcgatt cctggcacgg ctgcgagcaa taccaacatc
     3121 ataatgcgcg atgtctggcg ctggttatgg gtataagggg agctagctat tctgaatacc
     3181 atttagtcct cgtttacaac cttttgctgg gcggcttttt tggcctgaac gcgggcaata
     3241 gccgcagcaa ctgccgcttt gcgcggatca atctgttctt ctggctctgc attagcctgt
     3301 tgctgttcca gcttgcgtgc tttggcacgg gcaatagcag cttcgacggc ggctttgcgt
     3361 ggatcaacct gttcttctgg ttccgcatta gcctgttgtt gctgttccag cttgcgcgct
     3421 ttggcgcgtg caatagccgc ttcgacggca gctttgcgcg gatcgacctg ttgttctggc
     3481 tccgcattgg cctgttgctg ttccagcttg cgtgctttgg cgcgggcgat agcggcttcg
     3541 acggcggctt tgcgtggatc aacctgttct tctggttccg cattagcctg ttgttgctgt
     3601 tccagcttgc gtgctttggc acgggcaata gcagcttcga cggcggcttt gcgtggatca
     3661 acctgttctt ctggttccgc attggcctgt tgctgttcca gcttgcgcgc tttggcgcgt
     3721 gcaatagccg cttcgacggc agctttgcgc ggatcgacct gttgttctgg ttcggcatta
     3781 gcctgttgct gttccagctt gcgtgctttg gcacgggcaa tagcggcttc gacggcggct
     3841 ttgcgcggat caacctgttg ttctggctcc gcattggcct gttgctgctc cagcttgcgt
     3901 gctttggcgc gggcaatagc cgcttcaacg gcagttttac gtggatcagc aacggttgca
     3961 gcgtcgttag tttgctgcag ttctgcctgt ttcgctctgg cttgcgcttt acgggcttcc
     4021 cgtgctgcaa taattgcact gttatccggg cgttcgcccg ctttaatcac aataggctgt
     4081 gtagcctggg cctgtttctc tttcacccgc gccagagcgg cagcaatcgc atctttatct
     4141 ttggctgcag gttgaacggc tgcgctctta tgtcgttcaa ggcgagccgt tttttcgcgc
     4201 tccagacgag cctggcgcgc ttcgaaacgc gctttggctt ctgcggcgcg cttttcttcc
     4261 tgacgaatag ccgcaatttc agctttttcc tgacggaaat attgcaccag ggggatattg
     4321 ctcgggcaaa cccacgcgca agccccacat tcaatgcagt cggcaatgtt atgcgtggtg
     4381 gctttatcgt gttgctgacc tttgctgaac cagtacagct gttgcggcaa aagatccgca
     4441 gggcaggcgt cagcacaggc gctacaacgg atgcaacttt gttcttcctg tggttcgcca
     4501 agttcattgg cagagggagc caaaagacag ttggtaatct ttacgaccgg gacatccagc
     4561 cacggtaagg taaagcccat tagcgggccg cccataatca ccatttgatc ggcagagggg
     4621 cagaatccgg catcattcaa taaatgacgc actggcgtac ccagccgtgc ccagacgttg
     4681 cccgggcgag cgattgcttc gccagtcagg gtcacgacac gctcggtaat cggctcgcca
     4741 tcaataacgg cacgtttcac tgcataagcg gtgccgacgt tttgcattaa tacgccgata
     4801 tcggatgaac gcccgccatg tggaacctgc ttcccggtca gaatgtaggt taattgttta
     4861 gcaccgccag aaggatattt ggtgggaatc acccgcagag aaatatcgtg ggagtccgcc
     4921 agcaccgcgc gtagcatgga aatcgcctgc ggtttgttat cttcaatgcc gataagaatt
     4981 tcgcgcggct gcagaatatg cgcaaggatg cggataccct ctacgacctg agccgcgcaa
     5041 tcctgcatca aacggtcatc ggcggtaatg tacggctcgc actcagcagc gttgataatc
     5101 aacgtttcaa tcttatctcc gccgccctgc aatttaacgc ctgtcggaaa tcccgcacca
     5161 cccagcccgg caacaccaaa ctgatgaatg cgctcgatta actcttcgcg actgcgagag
     5221 cgataatcgg cccagccgtc gcgcgggatc cagcagtctt cgccatcggc atcaataatc
     5281 acacttaatt cagctaaagc tgaaggatga gccgtagagt ggggcgcaat agccgtaacg
     5341 gtacccgagg tgggcgcgtg aaccggcagc attttgccgc gaccacgggt aagcggctgg
     5401 ccgcgcaata ctttatcgcc gacgctaacg cacaactcac cttcagcgcc aatatgttgt
     5461 tttagtggaa tgacaaaacg ctgcgccagt ggtacctggc gcaggggcgt accgttggac
     5521 tgggttttca tctccggtgg atggatgccg ccgttgaaat cccagatttt attttttctg
     5581 aatgcagaga ataacttaag catggtgttc cacgggaatg atacgcacgg gaatggtgtt
     5641 cagatcccat ttccaggagt caggagtttc tgcgaccggt tgcaacgaga tgcagtgcgt
     5701 cgggcacgga tcaacacata aattgcagcc cgtacagaga tcactcatta ccgtatgcat
     5761 ggcgcgggta gcgccaacga tggcgtctac cggacacgcc tgaatacatt tagtgcagcc
     5821 aatacagtta ttttcatcaa taaccgccac catccgcgct ggcgttagct cttgcgcttc
     5881 gccatccagc ggctgcggct cgacattaag caactcggca atttttagca tcacagcttc
     5941 gccacctggg gcgcaacggt tgattttttc accgttacag ctgatggctt ccgcgtaggg
     6001 gcgacagccg ggataaccgc actgaccaca ctggctctgc ggtaagattt cgtcaatttt
     6061 ctcaacgacc ggatcgtctt ccaccgcaaa acggcgggag gcataaccca gaatggcgcc
     6121 aaacgccagg gccagcaggc tcacggcggc aacggcaatc cagatagcat tcattacaac
     6181 ttcaccaaac cactaaagcc cataaaggcc agagacataa gacctgcggt aattaacgca
     6241 atggcattac cgcgaaaagg tgccgggaca tcagccacag caaggcgttc gcggatggcg
     6301 gcgaagagca ccatcaccag cgagaaaccg acagcggcgg aaaaaccgta cagcgctgac
     6361 tgcaagaaat tgtgcccgag attgatattc agcaacgcca cgccgagcac ggcacagttg
     6421 gtggtgataa gcggcaaaaa aatccccagc agacggtaaa gtaccgggct ggttttgcgc
     6481 accaccattt cggtgaactg cacaaccaca gcaatcacca gaataaacgc cagggtgcgc
     6541 agataaatca gattaagtgg gatcaaaatc cacgtatcga taagccaggc gcaaatagac
     6601 gccagcgtca ttacaaacgt tgttgccagc cccatgccca tcgcggtttc cagctttttg
     6661 gaaaccccca taaacggaca gagaccgaga aacttgacca gtacaaagtt attgaccagt
     6721 acagttccga caaacagtaa caggtagtca gtcatttttc ggcctgaaat aaaaaagccg
     6781 cctattatcc gttaatccag agcaggcgac aacaggttaa ctgtaaggtt attacggatt
     6841 cacaaacgtc gctttcaccc gcgacgagcg tttgaaatag gggacgatca atgcggttgc
     6901 caacaaagta aacagcaact ggcgaacggc tatgccatct tctacgggtg aaaaagcgaa
     6961 cgcttttact gccagcaata cggaaataag cagccagata atatagtgtt taggaacaca
     7021 acgccgacgt ttaaagaaag cgatggtcaa ccacagtgtg taataccaca tggcgatagc
     7081 ggtaataaaa gaaacgcccc ataagatttg tgtcgtcagt gcttgtccgc cgagcgtttg
     7141 aaatgtttgc ggagaagata acgcagcggt atacagcaac aacgccagcg tcgtactcaa
     7201 cagagcgact aaaagccagg ccaacgggcc aagcaaccag cctccaatac gttgtggcgt
     7261 tgtggtggtc ataggttctc cagaatagcg acgcaaacag acaaatctaa ttttaggggc
     7321 gttattataa ggcttttata accgatcgct actttcttta ctggacatag tgccagacgg
     7381 acttcggtac ctggccgagg tcaaacaaac gcccgccaga aaccagctct gcgcgacggt
     7441 gatcggcagc acgatacata ttgatcagtt cctgatcgtc cgtcagggtg taattaagat
     7501 ggtcgtagag tttttccaga ctttcgagtg aactgatttt gcgaaatttt aataagtagt
     7561 cctgaacagt cataaaaggt ccataaataa ttaataccaa aggggtaggt attattagtg
     7621 atgataaaat ttcaccaaaa gtgaacgctt gtgttattac aataataaga atgcgctgat
     7681 ggcaaatatt cagggatgaa aaactgctct gaaaagagta acgcgcttta ctatccctga
     7741 caatcatcaa caacatcgaa caagataata aattcctggt ttaatatccg acaagtgaaa
     7801 acatgcaccc ggacgggcag catgtcgctc aacaagtgca gagcttactt gtgttgtacc
     7861 gtagtactct gttcaggtgg ctgatagttg tcaatgtgac tcgccacgcc aagaagaatg
     7921 actgaaacga caagaacgat ccaacctgtt aattcaataa gacgattcat tacagcccac
     7981 atctctgttg attgatccat taacttcagg gggtaaatgt tacttagcaa taatagctcc
     8041 agcaagattt ttactgaggt ttttgcgata tttagctttt gtcgttggaa aaatcgctat
     8101 ttttttgact taagttaaac aacatccctt attgctggca ggttattaaa ctgttgagcg
     8161 agggtaaagg atagtgtcaa atagccatca tacttcaatg agaggcaatg acatgagcga
     8221 caacatccgt gttggggtga ttgggtatgg ttatgcgagc aaaaccttcc atgcgcccct
     8281 gattgcgggc acgcccgggc tggaactggc ggtaatctcc agcagcgatg aaacaaaagt
     8341 aaaagccgac tggccaacgg ttacggttgt ctctgagccg aagcatctgt ttaacgatcc
     8401 caacatagac ctgattgtca ttcctacacc caacgatacc cattttccgt tagctaaagc
     8461 ggcgcttgag gcgggtaaac atgtggtcgt tgataaaccc tttaccgtga cactgtcaca
     8521 agcacgagag ctggatgcgc tggcaaaaac cctggggcgt gtgttgtctg tattccataa
     8581 ccgtcgctgg gatagcgatt ttctgaccct aaaaggtttg ctcgcggaag gcgtgctggg
     8641 ggaagttgct tactttgagt ctcattttga ccgcttccgt ccgcaggtac gcgatcgttg
     8701 gcgtgaacag ggcggcccag gcagcggtat ctggtacgat ttagcaccgc accttcttga
     8761 tcaggccatt acgctatttg gtttaccggt tagcatgacg gtagatttgg cacagttacg
     8821 gcccggagcg cagtcgaccg attatttcca cgctatcttg tcctatccgc agcggcgagt
     8881 gattttacac ggcaccatgc tggcagccgc tgagtcagca cggtatatcg tgcatggatc
     8941 ccgaggcagt tatgtgaaat atggcctcga tccacaggaa gaacgtctga aaaatggcga
     9001 gcgtctgccg caggaagact ggggctacga tatgcgtgat ggcgtactta cccgcgtgga
     9061 aggtgaggaa cgtgtcgaag aaacgctgtt gacagtgccg gggaattatc cggcttacta
     9121 tgcggctatt cgtgatgcgt taaatggcga tggtgaaaat ccggttccgg caagtcaggc
     9181 aatccaggta atggagttga ttgagctggg catcgaatcc gccaaacatc gcgcgacttt
     9241 gtgccttgcg tgaaaagggg atggtgcaat tagcaccatc catttttgtt acttcgcggc
     9301 gactttttct cgcagtgcgc gtttttcctc tgcgctgagg aaagccattt ccagaccatt
     9361 aatctgtgcc tggcggattt gctcgcggga taacccagca gctggcgcgg caacggtata
     9421 ttcgtgaatg atatccactc cctgcacgcc gggatcgtca gtgttaatgc tggcacgaat
     9481 gccatgctca aggaacgttt tcagcggatg tgcagccaga tccgccacgg tgctggtctg
     9541 aatattggag gtcagacagg attcaatacc aatttgttgc tcggcgagaa aatccatcag
     9601 cgcccgatct tcaatggctt ttacgccatg tccaatacgc tccgccccca gttcacgaat
     9661 cgcctgccag atgctttccg gtccggcagc ttcgcctgca tggacggtaa tatgccagcc
     9721 cgcatcacgc gcgcggttga aatgagaaag gaacagactt cccgggaaac caagttcatc
     9781 accggctaaa tcaagtgcgg taatctggtc acggtgggct aaaaaggcct ccagctcttg
     9841 ctgacaggcg gcttcgccga aggtccggct cataataccg ataagcttcg cctgcacacc
     9901 aaaggtgcgg caaccttcac gtacgccatc gatcaccgct tcgacaacac ccgctacagg
     9961 cagctgatgt gccattgcca tgtagcctgg tgaaaaacgc agttcgacat agtgcaggcc
    10021 gttacgggct gcatcttcaa tgttttcaaa tgccacgcgg cggcaggcat caagagaggc
    10081 gagaacttta acgccccagt caagtttagt cagaaagctc accagatcgg gttcgttggc
    10141 aatgacctga acgtggggag tcagtgtttc cagggattgt gcaggaagcg agatattata
    10201 ctggcggcca agttcaagaa tggtctgggg acgaatgttg ccatcaaggt ggcgatggat
    10261 atcagttaat ggcagggtgg tatcaatcat ggtcgcactc tttttctgga tataaaagtg
    10321 cgcaccatta taaaaacaaa gcagggtaaa aatccacgat tgcgcaacga ttttttacgt
    10381 tgcgcaattg gggttaacca acagcgcgga tggcgttaat taatccagcc acaccttttt
    10441 ccagtttcga acgtgggcag ccggcattga gtcggacaaa accacgacct tcttcaccgt
    10501 aggtatatcc cggcatgatc gcgatctttt cttgctcgat aagcgctttt tgcaacgcgt
    10561 tgtcgtcaat attcaacgga cgtagatcaa gccaggccag ataagtggat tgcgggatct
    10621 gccagttgag ttcaggaaac gcagcgttca ttttatctgc gatatacgtc aggttatctt
    10681 tcagatagac gcgtaaggca tccagccatg gcgcgccttg ctgataggcg gcgatatgag
    10741 cagttaacgc cagtaccgaa ggggaagaaa gcccatcacg tcctttcagt gccgataaat
    10801 aggcatcgcg gctactgcta ttttctataa tcccgtaagc accggtcagg gcgggaatat
    10861 tgaaactttt tgatcccgac gttagcaacg cccagtctcc gcgcgccaca ttactccagg
    10921 gaatatgcgg ctgctcgccc caaaccatat ccatatggat ttcatcggaa ataacccgca
    10981 caccatgacg ctcgcacagg tcagccatga tctccagctc atcgcacgtc cacactttcc
    11041 cggtaggatt ctgtgggcta cacaggagca taattttaca ttctggtttc gccagcacgg
    11101 cttccagctt gcccatatcg caaaaccagc cgtcagcctg cttctctaaa gcaacgggca
    11161 ttactgtgcg ctggttacct tcaatggcct tgtaaaatgc gtcataggcg ggtgtgtgga
    11221 tcaccacgcc ttcacctgtt tcagaccact gacgaatcag ttctgaaacc atatagataa
    11281 cagaagggcc atacaccacc gactgaggat cgatggcggt gtaatgctgg gtggaaaacc
    11341 agtgggcaat agccgcgaga aactcatcgt ttttccagcg gctgtagcca aacacgccgt
    11401 gtatcaggcg ctgattcagc gcctcgataa tgcagggggc agtggcaaaa tccatgtctg
    11461 aaatcgtgaa cggtaacagg tcagcagtgc cgaaacggtc agcgacataa tcccactgcg
    11521 tacaccatgt gccatgacga tccacgacct ttgaaaaatc gaacatatct tatccttatg
    11581 cctggacagt acgcatcaga ccgaccattt catcttttac tgactgaact tgtggcccga
    11641 taacaacctg caggttatgt tgattaagtt gtactacgcc aattgcccga ttgtccttca
    11701 gtgcctgcac attaacaagc gacatatctt ttacagacaa acgcagacgc gtaatgcagt
    11761 tatcgagact gacaatattg tcggcaccgc ctaaggcttc gaggattgcc ggaacgttgt
    11821 aacctgattt acccggcgca ccggcaacgg ctttttcgat tgaactggca acttcactat
    11881 cgcgccccgg ggttttgaga ttgaagcggg tgatagcgaa acggaagatg atgtagtaaa
    11941 cgacaaacca gattgccgcc actactggca ccatgtacca cttggttgac agaccatgca
    12001 aaataccgaa caccacaaag tcgatgatat tgccgtcggt attaccgatg gtgacgccga
    12061 gcacagacat gacagtgaag ccgaggccgg ttaacagcgc gtggatgaca tacagaactg
    12121 gcgctacgaa caggaacagg aattccagag gttcggtcgt gccgccaacg acacaggcga
    12181 tcaggccaga aatcagcaga cctttaattt tatggcgatt ttccgggcgc gcacagtgat
    12241 acatagctaa cgctgcacct ggcagaccgc cgagaaacgc aggcatttta ccttgcgaaa
    12301 ggaaacgcgt ggcgctttca gaaaaaccgt gagtggtcgg gcaactcaat tgcgcctgga
    12361 agatggtcag tgcaccgctg acggtttgac cgcagacttc ctgcgtgccg cctgcgtcgg
    12421 taaagcgaat taatgccacc agaatgtgat gcagaccaaa cggcaacagc agacgttcac
    12481 cggtaccaaa cagcatcggt ccgaaatcac ccgcgctgtt gatcatatgg cctaagccgc
    12541 taatacccat ggcgaaaatc ggccagacta atggaatcac caggccgaca agacccatca
    12601 ccagcgagga gatgattggt acgaagcgcg taccgccgaa gaatgccagc gcatccggca
    12661 ggcggatatt atggaatcgc tcatgcagca tccagacgat aataccggcg atcaccgcac
    12721 cgaggatccc ggtgtcgatc gactggatcc caagaatgct ctggatgtta ttggctttca
    12781 gaacggcggc gtccatggtt ggcagaatgc ctttattggt caaccagaag tttaccgcga
    12841 ggttcattac cgcataaccg acgaagccag cgaatgccgc tacgcctttg ttttcgcgtg
    12901 ccagacccag cgggatggcg atacagaaca tgactggcag gaaactaaaa gcaaacgagc
    12961 caatcttact catccaggta aagatagcct gcaacacggg gttgcccagg accgggatca
    13021 gggtgatgac atcatggctg ctaagagaac taccaatgcc gagcataatg ccgcagaacg
    13081 acaataatgc cacgggtaac atgaaggttt tgcctaactg ctggaagaac tcccacagcg
    13141 tgactttcgg tgctgttttc gccgtcataa aacgactcct cgtagagaac aatctggatt
    13201 cagtaaattg cgcgagaaga taaaacgttt tatcaaattt tagtgaggca taaatcacat
    13261 tacgcaacga taatagcggg tataagataa ataaaaggta aaacgtttta tctgtcacat
    13321 aatcagggag taggtcatct gcatggctac cgccaaaaaa ataaccattc atgatgttgc
    13381 gctggctgcg ggcgtgtcgg taagtaccgt ttcgctggtg cttagtggca aagggcgaat
    13441 ctctaccgcc acaggagaac gcgttaacgc cgccattgaa gagctgggat ttgtgcgcaa
    13501 tcggcaggcg tcggcgctgc gcggcgggca aagtggcgtc attggtttga tcgtccgtga
    13561 tttatctgcg ccgttttacg ccgaattgac ggccggattg acggaagctc tggaagcgca
    13621 gggacggatg gtttttttgc ttcacggcgg taaagacggc gagcagctgg cacagcggtt
    13681 ttcactatta ctgaatcagg gtgtcgatgg tgtggtaatt gccggggctg caggaagcag
    13741 cgatgacctg cgacggatag cagaagaaaa agctatcccg gtgattttcg cttcccgtgc
    13801 cagttatctt gatgacgttg atacggttcg cccggacaac atgcaggctg cacagttgtt
    13861 gacggagcat ctcattcgca atgggcatca gcgtatcgcc tggctgggag ggcaaagttc
    13921 ctcattaacc cgggcagaac gggtgggggg ctattgtgcg actctactaa aatttggcct
    13981 gccgtttcac agcgattggg tgctggagtg cacttccagc cagaagcaag ccgcggaagc
    14041 tatcacggcg cttttacgtc ataacccgac catcagcgcc gtggtttgct ataacgaaac
    14101 tattgcgatg ggggcatggt ttggtttgct gaaagcagga aggcaaagcg gggaaagcgg
    14161 agtcgatcgt tactttgagc aacaggtttc gctggcggca tttaccgatg caacgcctac
    14221 cacacttgat gatatacccg ttatctgggc cagcacgcca gcgcgggaac ttggtaccac
    14281 acttgcggat cgcatgatgc aaaaaatcac ccatgaagag acgcattcac gcaatcttat
    14341 tattcccgcc cggctcattg cggcgaaata atcctctctt tatctgctat acctggtagt
    14401 gtcctttcct caaggttaat gataaaaaat gtggcacaca ggcggtgcct tttattttta
    14461 ctgaagttca caggaggttt atgtgtttaa ttctgacaac ctgagactcg acggaaaatg
    14521 cgccatcatt acaggtgcgg gtgcaggtat tggtaaagaa atcgccatta cattcgcgac
    14581 agctggcgca tctgtggtgg tcagtgatat taacgccgat gcagctaatc atgttgtaga
    14641 tgaaattcaa caactgggtg gtcaggcatt tgcctgccgc tgcgatatta cttccgaaca
    14701 ggaactctct gcactggcag actttgccgt cagtaagttg ggtaaagttg atatcctggt
    14761 taacaacgcc ggtggcggtg ggcctaaacc gtttgatatg ccaatggcag attttcgccg
    14821 cgcttatgaa ctgaatgtat tttctttttt ccatctgtca caacttattg cgccagaaat
    14881 ggaaaaaaat ggcggtggcg ttattctgac cattacttct atggcggcag aaaataaaaa
    14941 tataaacatg acctcctatg catcatctaa agctgcggcc agtcatctgg tcagaaatat
    15001 ggcgtttgac ctgggtgaaa aaaatattcg ggtgaatggc attgcgccgg gggcaatatt
    15061 aaccaatgcc ctgaaatccg ttattacccc agaaattgaa cagaaaatgt tgcaacacac
    15121 accaatcaga cgtctgggcc aaccgcaaga tattgctaac gcggcgctgt tcctttgttc
    15181 gcctgcagcc agctgggtaa gcggacaaat tctcaccgtc tccggtgatg gggtacagga
    15241 actcaattaa tacactaacg aaccggtaaa cagccgtatg tgttgtttac cgggatagcg
    15301 tgtgcaactc gtcaacgtct ctgctaaaca actggcagcc aaatcacggc tattggttaa
    15361 ccaatatctg gataaaaagc ttacgaattg agtgtgcctt cgcactattc aacagcaatg
    15421 ataggcgctc acctgacaac gcggtaaact agttattcat gctaactata atggtttaat
    15481 gatggataac atgcagactg aagcacaacc gacacggacc cggatcctca atgctgccag
    15541 agagattttt tcagaaaatg gatttcacag tgcctcgatg aaagccatct gtaaatcctg
    15601 cgccattagt cccgggacgc tctatcacca tttcatctcc aaagaagcct tgattcaggc
    15661 gattatctta caggaccagg agagggcgct ggcccgtttc cgggaaccga ttgaagggat
    15721 tcatttcgtt gactatatgg tcgagtctat tgtctctctc acccatgaag cctttgggca
    15781 acgagcgctg gtggtcgaaa ttatggcgga agggatgcgt aacccacagg tcgccgccat
    15841 gcttaaaaat aagcatatga cgatcacgga atttgttgcc cagcggatgc gtgatgccca
    15901 gcaaaaaggc gagataagcc cagacatcaa cacggcaatg acttcacgtt tactgctgga
    15961 tctgacctac ggtgtactgg ccgatatcga agcggaagat ctggcgcgtg aagcgtcgtt
    16021 tgctcaggga ttacgcgcga tgattggcgg tatattaacc gcatcctgat cctctctctc
    16081 tttccgcggg ctggtgacaa ctgagcccgc gtttcatatc gtaatttctc tgtgcaaaaa
    16141 tcatcctccc agggatcgga gaattccacc taaatattca tcgtagcaat atatcatgag
    16201 ggtttatcgt tcccaatatg ttcgaatgaa cgttcgattg cttattttgt ggcttctgtc
    16261 aacgctgttt taaagattaa tgcgatcaat atcacgctgt gggtattgca gtttttggtt
    16321 ttttgatcgc ggtgtcagtt ctttttattt ccatttctct tccatgggtt tctcacagat
    16381 aactgtgtgc aacacggaat tggttaacta atcagattaa aggttgacca gtatttttat
    16441 cttaatgagg agtcccttat gttacgtcct gtagaaaccc caacccgtga aatcaaaaaa
    16501 ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga tcagcattgg
    16561 tgggaaagcg cgttacaaga aagccgggca attgctgtgc cgggcagttt taacgatcag
    16621 ttcgccgatg cagatattcg taattatgtg ggcaacgtct ggtatcagcg cgaagttttt
    16681 ataccgaaag gttgggcggg ccagcgtatt gtactgcgtt tcgatgcggt cactcattac
    16741 ggcaaagtgt gggtaaataa tcaggaagtg atggagcatc agggcggcta tacgccattt
    16801 gaagccgatg tcacaccgta tgttattgcc gggaaaagtg tacgtatcac tgtttgtgtg
    16861 aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga cgaaaacggc
    16921 aagaaaaagc agtcttactt tcatgatttc tttaactacg ccgggatcca tcgcagcgta
    16981 atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac gcatgtcgcg
    17041 caagactgta accacgcatc tgttgactgg caggtggtgg caaatggtga tgtcagcgtt
    17101 gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcaccag cgggactttg
    17161 caagtagtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc
    17221 acagccaaaa gccagacaga gtgtgatatc tacctgctgc gcgtcggtat ccggtcagtg
    17281 gcagtgaagg gcgaacagtt cctgatcaac cacaaaccgt tctactttac tggctttggt
    17341 cgtcatgaag atgcggattt gcgcggcaaa ggattcgata acgtgctgat ggtgcacgat
    17401 cacgcactaa tggactggat tggggccaac tcctaccgta cctcgcatta cccttacgct
    17461 gaagagatgc tcgactgggc agatgaacat ggaatcgtgg tgattgatga aactgcagct
    17521 gtcggcttta acctctcttt aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg
    17581 tacagcgaag aggcagtcaa cggggaaacc cagcaggcgc acttacaggc gattaaagag
    17641 ctgattgcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc caacgaaccg
    17701 gatacccgtc cgcaaggtgc acgggaatat tttgcgccac tggcggaagc aacgcgtaaa
    17761 ctcgacccga cgcgtccgat cacctgtgtc aatgtaatgt tctgcgacgc tcacaccgat
    17821 accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggttg gtatgtccaa
    17881 agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc ctggcaggag
    17941 aaactgcatc agccgattat catcaccgaa tacggtgtgg atacgttagc cggcctgcac
    18001 tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac
    18061 cgcgtctttg atcgcgtcag cgctgtcgtc ggtgagcagg tatggaattt cgccgatttt
    18121 gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt cactcgcgac
    18181 cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggaccggcat gaacttcggt
    18241 gaaaaaccgc agcagggagg caaacaatga atcaacaact ctcctggcgc gccatcgtcg
    18301 gctacagcct cggtgacgtc gccaataact tcgccttcgc aatgggggcg ctcttcctgt
    18361 tgagttacta caccgacgtc gctggcgtcg gtgccgctgc ggcgggcacc atgctgttac
    18421 tggtgcgggt attcgatgcc ttcgccgatg tctttgccgg acgagtggtg gacagtgtga
    18481 atacccgatg gggaaaattc cgcccgtttt tactcttcgg tactgcgccg ttaatgatct
    18541 tcagcgtgct ggtattctgg gtgccgaccg actggagcca tagcagcaaa gttgtgtatg
    18601 catatttgac ctacatggga ctcgggcttt gctacagcct ggtgaatatt ccttatggtt
    18661 cacttgctac cgcgatgacc caacaaccac aatcccgcgc ccgtctgggc gcggctcgcg
    18721 ggattgcgct tcattgacct ttgtctgcct ggcatttctg ataggaccga gcattaagaa
    18781 ttccagcccg gaagagatgg tgtcggtata tcatttctgg acaattgtgc tggcgattgc
    18841 cggaatggtg ctttacttca tctgcttcaa atcgacgcgt gagaatgtgg tacgtatcgt
    18901 ggcgcagccg tcattgaaga tcagtctgca aaccctgaaa cggaatcgcc cgctgtttat
    18961 gttgtgcatc ggtgcgctgt gtgtgctgat ttcgaccttc gcggtcagcg cctcgtcgtt
    19021 gttctacgtg cgctatgtgt taaatgatac cgggctgttc actgtgctgg tactggtgca
    19081 aaacctggtc ggtactgtgg catcggcacc gctggtgccg gggatggtcg cgaggatcgg
    19141 taaaaagaat accttcctga ttggcgcttt gctgggaacc tgcggttatc tgctgttctt
    19201 ctgggtttcc gtctggtcac tgccggtggc gttggttgcg ttggccatcg catcaattgg
    19261 ccagggcgtt accatgaccg tgatgtgggc actggaagct gataccgtag aatacggtga
    19321 atacctgacc ggcgtgcgaa ttgaagggct cacctattca ctattctcat ttacccgtaa
    19381 atgcggtcag gcaatcggag gttcaattcc tgcctttatt ttgggattaa gcggatatat
    19441 cgccaatcag gcgcaaacgc cggaagttat tacgggcatc cgcacatcaa ttgccttagt
    19501 accttgcgga tttatgctac tggcattcgt cattatctgg ttttatccgc tcacggataa
    19561 aaaattcaaa gaaatcgttg ttgaaattga taatcgtaaa aaagtgcagc agcaattaat
    19621 caacgatatc actagttaat attcaataaa aataatcaga acatcaaagg tgcaactatg
    19681 agaaaaatag tggccatggc cgttatttgc ctgacggctg cctctggcct tacctctgct
    19741 tacgcggcac aactggctga cgatgaagcg ggactacgca tcagactgaa aaacgaatta
    19801 cgcagggctg ataagcccag tgctggcacg ggaagagata tttacgcatg ggtacaggga
    19861 ggattgctcg atttcaatag tggttattat tccaataatt attggcgttg aaggcggggc
    19921 gtattatgtt tataaattag gtgctcgtgc tgatatgagt acccggtggt atcttgatgg
    19981 tgataaaagt tttggctttg ccctgggggc agtaaaaata aaacccagtg aaaatagcct
    20041 gctaaaatta ggtcgcttcg ggacggatta tagttatggt agcttacctt atcgtattcc
    20101 gttaatggct ggcagttcgc aacgtacatt accgactgtt tctgaaggag cattaggtta
    20161 ttgggcttta acgccaaata ttgatctgtg gggaatgtgg cgttcacgag tatttttatg
    20221 gaccgattca acaaccggta ttcgtgatga aggtgtttat aacagccaga cgggaaaata
    20281 cgataaacat cgcgcacgtt cttttttagc cgccagttgg catgatgata ccagtcgcta
    20341 ttctctgggg gcatcggtac agaaagatgt ttccaatcag atacaaagta ttctcgagaa
    20401 aagcataccg cttgacccga attatacgtt gaaaggggag ttgctcggct tttacgcgca
    20461 acttgaaggt ttaagtcgta ataccagcca gcccaatgaa acggcgttgg ttagtggaca
    20521 attgacctgg aatgcgccgt ggggaagcgt atttggcagt ggtggttatt tgcgccatgc
    20581 aatgaatggt gccgtggtgg ataccgacat tggctatccc ttttcattaa gtcttgatcg
    20641 taaccgtgaa ggaatgcagt cctggcaatt gggcgtcaac tatcgtttaa ctccgcaatt
    20701 tacgctgaca tttgcaccga ttgtgacccg cggctatgaa tccagtaaac gagatgtgcg
    20761 gattgaagga gcgggtatct tagggggtat gaactatcgg gtcagcgaag ggccgttaca
    20821 agggatgaat ttctttcttg ctgccgataa aggacgggaa aagcgcgatg gcagtacgct
    20881 gggcgatcgc ctgaattact gggatgtgaa aatgagtatt cagtatgact ttatgctgaa
    20941 gtaagaaaaa acgccggaga gaaaaatctc cggcgttttg gctgtgaatt actgctgcgg
    21001 aatagcgggt acagccggaa cgtttaatgt cggcatcgca aacataccca cgaaatcttc
    21061 cagtggcatt ttctgcccgt ttaacgttat ctgaccgtta gcatattgca ggctggtggt
    21121 gatggtattg tcctgcaagg tggtcagacg gaacatctgc cccattgctg atgcaccttc
    21181 aacttgctgt ttcgccagtt ttttcgcttg atcttcctga taaccttcca gcttcgctac
    21241 ctgagtcatc aactcagttg ccatatccac cggaatggtc agtttcgcat ccagagattt
    21301 aaccgagcgg tccacttcct gcgccagcgt ttgcggcgct tctttagtcg ttgccggatc
    21361 tttcaggaac agcgacagat tcagcgcact ttcaccctga ctgtttttcc agcttagcgg
    21421 cgcgatagta atcaccggat cgcctttcag catcagcggc agagcgctaa agaaggcttc
    21481 cgtcactttc tcctgataaa gttcgggatt gttggcaatt tctggctgtg ccagcagcgc
    21541 ctgagtttgc gcgttatatt gctggctaaa ctgatgccag gcttcgccat caatttggcc
    21601 gacttttaaa gtcagcttgc cactgcccag atcctgattc tgtaccttca ggctgtttag
    21661 cgagtaatcc agttggctat tgatcgtttt accgtcattg accagatccg atttaccgct
    21721 gatctccatg ccttccagca gtgccagttc tttgccctcc actgaaatgg tcattttttc
    21781 cagcgacagt ttttggtttc ctacgcgctc accaaaactt gccagcgtgc tggaaccgtc
    21841 ggttttcaga ttattaaagg ttaactgcac tttctggttg tattcgttaa ccgcgtctat
    21901 ccgaccactt tgcgcctccc cggaaaggga gatagctttg ccgtctctgt ctgcatttaa
    21961 ctggaactcg ccgccgctaa aggcgacttt ttcatccttt tgctcgtaat tcagtggctt
    22021 gagcgaaatg tcggaactgg aatctccgct gtaaccaatg cgggagttaa tctcaaaagg
    22081 cgtttcacct tttgccatat caaacagtgg tttgcttact tcgttattaa ccagcgtggt
    22141 ttgaattgat gccatcgacg ggatcagatt cagtttttta agctgggcaa gcgggaaggg
    22201 accatgatca accgattcgt tgaagatgac gctctgaccg cttttaatcc acggatttac
    22261 ttttccggca atgggtttca ccaacagttg caactggctg ctgaatacgc cgcgatgata
    22321 gttttgataa ctgacttcca ggttggattc aggagctgtc agtttgagtt gcgcgttcgc
    22381 ctgcgcgacc atgtcttcga gatgggtttc aatcttcttg cctgtatacc atgcgccgcc
    22441 tgtccagact acgcctagcg caacaatgac gcctaccgct accagcgatt tattcataat
    22501 gattatccat aaaatgaaat caggcggact ggccgcctga aggtgttata agcctttaat
    22561 aagcttagca agagatgtta attttttcag taagctctta cagcttgttg taaacacgcg
    22621 ctaaacggcc gtggcctttg acagtcaccg gtgattcgtt ggcggcaata aacgctgatt
    22681 cacccggttt aagctgtaac tgctgagaac ctttacacag cgttgcatcg ccttcgacgc
    22741 agaacaaaat ggcggcactc tgctggctga tggtggtttc tttatcacta aggtcatgca
    22801 acgagaaggc aaaatcatcc acaggaatcg ggaagtccag ttctgcacct tgtttcaccg
    22861 gctgggtcaa caacttgtta gccggtttgg cttcgaactt cacgttggca accagttccg
    22921 gaatatcaat gtatttaggc gtcagacctg cacgcagcac gttatcggag ttcgccatca
    22981 cttccagcgc cacgccttgc aggtaagcgt gcggtgtttc agcgaacagg aacatcgctt
    23041 cgccaggatt caatttcacc acattcagca acagcgggga gaacaggccg ctgtcttccg
    23101 ggtaaaattc agaaattaaa cgaatcgttt gccatggttc accctgctgg ctgtcgaggg
    23161 ccgattttaa aatcgccagc gcgcgggatt tttcttcacc ctgcatattc aacaggctgg
    23221 cgaacagttc gcttaaacgt tcggcatcag gctgttgtaa aaagtgagca atcgccggat
    23281 gtgcacctgc gaccggctgg agcagggaga caatctcgga aaactcacga aacgcgttca
    23341 tcgcgaggaa aggggtcagg gcgaaaacca gctcaggctt gtgattagga tctttatagt
    23401 tacgctcagc ggcatccatc ggaatacctg cagcattttc tttggcaaaa ccgatttcag
    23461 aattgcgttt gtttggatga acctgaatgg agagcggctg ggctgcgcat aatactttga
    23521 acaggaaagg caactcgcca aagcgtttgg caacggcatc tcccaaaaga gtcgctttat
    23581 cactctcaat cacatcacgc agtgaaacga tatctcctgc ggcatcgtgc acacgtgaac
    23641 tgctctttgg atgcgcgccc atccacaact cggccatcgg ctggctggac ggattttcca
    23701 taccataaag ttcagtcaac gccgttttgc tgccccaggc atagttttgc actgagttaa
    23761 tgagtttttg catgatcaat ccctgtttta atgtggaaat taatcccgct attaaagcaa
    23821 gaatcctacg ggaagtaacc tggagccgca aaaagtcgta ctagtctcag tttttgttaa
    23881 aaaagtgtgt aggatattgt tactcgcttt taacagggca acggaacacc cgcccagagc
    23941 ataaccaaac caggcagtaa gtgagagaac aatgtcaaac aaaccctttc attatcaggc
    24001 tccttttcca ctcaaaaaag atgatactga gtattacctg ctaaccagcg aacacgttag
    24061 cgtatctgaa tttgaagggc aggagatttt gaaagtcgca cccgaagcgt taactctgtt
    24121 ggcgcgtcag gcgtttcatg atgcgtcatt tatgctgcgt ccggctcacc aacaacaggt
    24181 ggccgacatt ctgtgtgacc cggaggccag cgaaaatgat aaatatgtgg cgctgcaatt
    24241 cctgcgtaac tccgacatcg cggcaaaagg cgttctgcca acctgtcagg ataccggcac
    24301 cgcgattatt gttggtaaaa aagggcagcg tgtatggacc ggtggcggtg atgaagcagc
    24361 gctggcgcgc ggtgtttata acacttatat cgaggataat ctgcgctact cgcaaaacgc
    24421 gccgctggat atgtataaag aggtgaatac cggcaccaac ttgccagcgc agatcgatct
    24481 ttatgccgtt gatggcgacg agtacaaatt cctctgtatc gccaaaggtg gcggttcggc
    24541 aaacaagacg tatctctatc aggaaaccaa agcgttactg acgccgggaa aactgaaaaa
    24601 ttacctggtt gagaagatgc gcacgctggg tacggcggcc tgtcctccgt atcatattgc
    24661 gttcgttatt ggtggaactt ctgcagaaac gaatcttaaa acggtgaaac tggcttccgc
    24721 taaatactat gatgaactgc caacggaagg gaatgagcac ggtcaggcgt tccgcgatgt
    24781 ggaactggaa aaagaattgc tgatcgaagc gcaaaatctt ggtctgggtg cgcagtttgg
    24841 tggtaaatac ttcgctcatg acatccgcgt gattcgcctg ccacgtcacg gcgcatcctg
    24901 cccggtcggt atgggcgtct cctgttctgc tgaccgtaat atcaaagcga agatcaaccg
    24961 tcaggggatc tggatcgaaa aactggaaca taatccaggc aaatatatcc cggaagagct
    25021 gcgcaaagca ggagaaggcg aagcggtgcg cgttgacctc aaccgtccga tgaaagagat
    25081 cctcgcacag ttgtcgcagt atcccgtttc tacacgctta tcgcttaacg gcacgattat
    25141 cgtcggtcgt gatattgctc acgccaaact gaaagagcgg atggataacg gtgaagggct
    25201 gccgcagtat attaaagatc atccgattta ctacgcgggt ccggccaaaa cgccggaagg
    25261 ttatgcctcc ggttctcttg gcccaacgac cgccggacgg atggattctt atgtcgatca
    25321 actgcaagcg cagggcggaa gtatgatcat gctggcgaaa ggcaaccgca gccagcaggt
    25381 gacggatgcc tgtaaaaaac acggcggctt ctacctcggc agtatcggtg gtccggcagc
    25441 tgtattggcg cagggaagta ttaagagcct ggaatgtgtt gaatatccgg aactgggaat
    25501 ggaagccatc tggaaaattg aagtggaaga tttcccggcg tttatccttg tggatgataa
    25561 aggaaatgac ttcttccagc agatacaact cacacaatgc acccgttgtg tgaaataaac
    25621 agagccgccc ttcggggcgg tttttttaca tggcacgaaa gaccaaacgt ttgttatcaa
    25681 atggtaaata atgagtgagc taaaagttgc ttaacgaaag caaaacagaa agaaaaaatt
    25741 aatcaggtga ggagcaggcc atgaatacag tacgcagcga aaaagattcg atgggagcga
    25801 ttgatgtccc ggcagataag ctgtggggcg cacaaactca gcgctcgctg gagcatttcc
    25861 gcatttcgac ggagaaaatg cccacctcac tgattcatgc gctggcgcta accaagcgcg
    25921 cagcggcaaa agttaatgaa gatttaggct tgttgtctga agagaaagcg agcgccattc
    25981 ggcaggcggc ggatgaagta ctggcaggac agcatgacga cgaattcccg ctggctatct
    26041 ggcagaccgg ctccggcacg caaagtaaca tgaatatgaa cgaagtgctg gctaaccggg
    26101 ccagtgaatt actcggtggc gtgcgcggga tggaacgtaa agttcaccct aacgacgacg
    26161 tgaacaaaag ccaaagttcc aacgatgtct ttccgacggc gatgcacgtt gcggcactac
    26221 tggcgctgcg caagcaactc attccacaac ttaaaaccct gacccagacg ctgagtgaaa
    26281 aatcccgcgc atttgctgat atcgtcaaaa tcggtcgtac ccacttgcag gacgccacgc
    26341 cgttaacgct ggggcaggag atttccggct gggtagcgat gctggagcat aatctcaaac
    26401 atatcgaata cagcctgcct cacgtagcgg aactggctct gggcggtaca gcggtgggta
    26461 ctggactaaa tacccatccg gaatatgcgc gtcgcgtagc agatgaactg gcagtcatta
    26521 cctgtgcacc gtttgttacc gcaccgaaca aatttgaagc gctggcgacc tgtgatgccc
    26581 tggttcaggc gcacggcgcg ttgaaagggt tggctgcgtc actgatgaaa attgccaatg
    26641 atgtccgctg gctggcctct ggcccgcgct gcggaattgg tgaaatctca atcccggaaa
    26701 atgagccggg cagctcaatc atgccaggga aagtgaaccc aacacagtgt gaagcattaa
    26761 ccatgctctg ctgtcaggtg atggggaatg acgtggcgat caacatgggt ggcgcttccg
    26821 gtaactttga actgaacgtc ttccgtccga tggtgatcca taatttcctg caatcggtgc
    26881 gcttgctggc agatggcatg gaaagtttca acaaacactg cgcagtgggc attgaaccga
    26941 atcgtgagcg aatcaatcaa ttactcaatg aatcgctgat gctggtgact gcgcttaaca
    27001 cccacattgg ttatgacaaa gccgccgaga tcgccaaaaa ggcgcataaa gaagggctga
    27061 ccttaaaagc ggcggccctt gcgctggggt atcttagcga agccgagttt gaccgctggg
    27121 tacggccaga acagatggtc ggcagtatga aagccggggg ttaatctgca acatacaggt
    27181 gcagccgtgg aatgattaaa cgaagcggct gcgcctgtgg tttataacgg tgctgcacat
    27241 tgtcggcatc gtaatttaac aactcaccaa cgtccggcac gcccgcgcca ttatcccgat
    27301 taatcagcgc aatcagcggc gtagggcagg cgtgttgtac ttgtttttga tctcccttat
    27361 accagacgcg ggcaatcggc tgcaccttca ccggacgttt gatttttaac ttcgcgttct
    27421 gtggcagggc agcgatatcc tgatattctc gctccagttt tctttgccac tcctcgcgcg
    27481 tccacggtgc gacactgcgt ggtgatttca ggcttttttc cagctgtgcc aggacttcat
    27541 cacgatgcaa atttttaatg atatgtttat tagcccagcc aaaacgtaaa gtggcggggt
    27601 cgtgcagaac ggtgagcgtg cggtaagcat taagggtaat cagccccgga aaatgacgat
    27661 gtacccattc aaaacgtgcc acggagggga gttctgattc aaccgtgacg atatgctcga
    27721 acgtggtctt gagtttattg atgtgctgaa tatgactgac caacgctgct tgcgaaaagt
    27781 tatcgacctg gtaacacaac acgccaggca gacggacggc ggctttgctg ctgcgatttt
    27841 cggactgttg ttgaataaat aaatggcgga aatgacgcaa cgccagcgac tgcgcgtcgt
    27901 tgccgagatg ttgttttacc tcaatacgat taagcggatt atgctcatcc tcttttttta
    27961 cctccggcaa agagaacacg cgggcaacca atagcttgtg ttgctcaaga tgagcggcaa
    28021 atgcagccag ctcttgttcc atctggcgaa aagtagtgtt gagtcggtct acgagatcgt
    28081 aacgcgccat aatattaatc actttagtta caacatcctt attttatagc acagtggtgg
    28141 tgacttgacc accgtgcgca tagtgtcagg cagaggtaaa ttgcgggatg ttatgccata
    28201 acggccagct gaacgagaag cgggcacccc ccagttcgct ggtgtcacaa ttaaccgtac
    28261 cgcccattgc cagtgctata gagtggacaa tggccagccc cagcccgcag ccgccggttg
    28321 agcgatcccg gctgggatcg aggcgaacaa aaggttcaaa gatatgttcg cggttttctg
    28381 gggcaatccc tgggccatca tcctcaacaa ttaatgtcgc tctattcccc gacagtagca
    28441 ggctggtttc aaccgttgag tgacagtagc gcagggcgtt attgagcaaa ttatccagca
    28501 cgcgctccat taagcgcata tccaacgcag cataatggcc ttgcgcgagc gtttttatcc
    28561 gtaccgtttt atcgggcgtt actgcctgaa tatctgccag atgcgttgac agccacaacg
    28621 gcaggtctgg ttcgctaaga tgaagctcat tttgtgggcg atcgagccgg gcataagtca
    28681 gcagctcttc aattaaagct tcaagttgac tgatatcgcg attcaacgcc tgggattcgg
    28741 cggcgctcag gttatcgctc atctccagtc gataacgcag gcgcactaac ggtgttcgca
    28801 gttcgtgagc gataccgtca ataagctgtt ttttgctggc aatcaaggcg ttgatattgt
    28861 ccgccatctg gttaaatgcg acgccaagtc gttcaaagct cgaaccttca tcaaagtgga
    28921 tacgctcact gagatgccca tcgccaaatc gttgcgccgc tgcttccagt tttaacatat
    28981 cctgccagtg cggacgcatc cagataaaca ccggaaaggc gagggaaatg gcaataaaag
    29041 cgatcagggc gatatccagc aatcgcatct gatggaggta ataaagataa ggaacaggac
    29101 caactgccag cacgtagtgg ctgcgtggga tacgttgcaa aaaagtgtac tgatcgtcca
    29161 gagcgacaat ttcgccgcca cgcagccggt gcatggaaat atcatcaaga tgatatttac
    29221 tcagcggctc gacacgcaga tcgaaagaga gatttaaatc catctctttc agcgtcttac
    29281 cccagtcgtg tggggggatc tcacgcaatt cgctgcgcat cagatacagc gaactgttca
    29341 tcaaatcatc cagcgactgt ttgcctgcgc gttcggcggt aaatttgtac accagcccga
    29401 ccagcagaga catcacaagg aagcagacaa acaataacag gtaaaactgg ataaacagtt
    29461 ttttcatcgc ttattcccac gcatgaggcg caaaaagata gcctttgtta cgcacggttt
    29521 taatacgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    29581 cgtccacgct acgatccagc ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    29641 cgcggtccat gatttgcccg gcatgggtag ctaattccca caataattcg aaatcagctg
    29701 tcgagagcga gatttcagtg ttagccaggg agactacgcg gttgatggga tcgatggtca
    29761 acgtgccgaa atgcagggct ttgtagggag tcagagacgt ttcctgaata cctttggtca
    29821 ctgtggcttg ctcattctga cgcaaatgca agcgtaaacg cgctaacaaa acagcagggg
    29881 gcgtcgtttt gagaatatag tcgcaggcgc ccatttccag tgccaggatg tggttcatat
    29941 cgctatcgag agaggttaga agaacaatcg gtccggacca ctttgcgcgt aaatcacgac
    30001 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    30061 ctcgcagaat agtttcttcg gcccggtcgc cgcgcggctc tacggtaacc tgcatatcat
    30121 gttttgccag gtatgcggca atcagtgcac cgacttccgc atcatcttcc acaaatacga
    30181 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgca ctgtttttac
    30241 ttactatcaa tcgtttctaa acaaggtttc atcaggtatt ctactgatga cttgttatgg
    30301 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    30361 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    30421 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    30481 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggcg
    30541 tcgctatggt atttcaccgg aatgatgcgc ttgcccgctg cttttgttgg ctctgttgcg
    30601 tgctggggaa taagcgcatg ggtgttgatt atatgctgga ttaagctgca ttaacgcaga
    30661 tgacgaccgc catcaagtgg gagactgcgt ccggtgacaa agcagctggt aagtaagtaa
    30721 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    30781 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    30841 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    30901 gccgctttgc ttgcagcata ggcaatatgt ttgtcgctac cgcgctccac cacataatcg
    30961 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagagca
    31021 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggtc
    31081 agtggcgctc ccggtttttc cgccatccac gcgctggcgt tatgcaaaat agcacgcaga
    31141 ccatgggtgc tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    31201 tcagcctgaa tacactgcgc acctgcctta atcagtccat caatggcggg atagtgcgtc
    31261 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    31321 atgcggcgac ctccgccagt aattaatatt ggcaaggact gggttttacc catcgttatc
    31381 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    31441 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    31501 gtatgcgttt tacgcgcgta caggaaaacc agcagccccg gcgcatacag aacaacggat
    31561 aacagcaagt gcattggacc agaggcgtac agtaaccata agccataaat gcaggcaccg
    31621 acacctaccg ccttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    31681 aaatagggca ccagaatcat ttctgaggcg atggtcagca atgtgttgta atcggaaccg
    31741 gtcagccaga tgagcaccag acaaatttgc acgcagatgt tggtcagcca cagtgaagcg
    31801 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgcg ggaatgcttt atgagtggct
    31861 gcaaggaacg gaacttctgc tgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    31921 acgatcaaac cggcagcgat gatgatttcg ccccacggtc ccatcatttc caccatcaga
    31981 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac gcccagcgaa
    32041 agcagcgtta ccagcaaata aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    32101 ccaacatcac gcttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    32161 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccacacggg tacgccaagt
    32221 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    32281 acaaacagac ccaacggcaa caattttgcc agtgtcgcca caaggttaat gctggcagcg
    32341 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgacg
    32401 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    32461 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    32521 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    32581 ataccgccgt caagttcggg gcgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    32641 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    32701 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    32761 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgacaag
    32821 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    32881 tacgaagaga tgtgtctgca tgcaatggtc gcagggaaat ttatgactaa aaatgcgaaa
    32941 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    33001 cggtcaggtt attaccacgt tcctgccact ggcgggtgat gtggtaatac ttagcacctt
    33061 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    33121 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    33181 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    33241 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    33301 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    33361 ttacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    33421 tacgctggtt accgccctgg ttggcatcga tttgacgaac aatgtagaaa gagtaggcac
    33481 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    33541 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    33601 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    33661 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    33721 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    33781 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    33841 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    33901 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    33961 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    34021 atttttcata tcacattcct taaaccaatt ttaattctgc tcaaatgacc gtctatgctt
    34081 aaaaaacagc cgtatcacca tcaatactac tgaagcaact gaattgtata agttaattta
    34141 atgttaagta gtgatttgtg ccggggcgat gtctcgtttt acccggccgc cgaagacaat
    34201 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    34261 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    34321 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    34381 gagcggcttg tgtggctcct gacacaagca aaccatcatc aataaaaccg atggaaggga
    34441 atatcatgcg aattggaata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    34501 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    34561 cgggtcaact ggcaagtttt gacgataagg cgtttgtgca agcgggcgct gaaattgtag
    34621 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    34681 aaattgcgtt actgaatcct gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    34741 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    34801 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    34861 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccgga caaatcactg
    34921 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtc gcaggtctgg
    34981 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    35041 aagtgaaaga acaagttcaa agtatgggcg cagaattcct cgagctggat tttaaagagg
    35101 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    35161 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    35221 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    35281 gtgtgattgt cgacctggca gcccaaaacg gcggcaactg tgaatacacc gtgccgggtg
    35341 aaatcttcac tacggaaaat ggtgtcaaag taattggtta caccgatctt ccgggccgtc
    35401 tgccgacgca atcctcacag ctttacggca ctaacctcgt taatctgctg aaactgttgt
    35461 gcaaagagaa agacggcaac atcactgtgg attttgatga tgtggtgatt cgtggcgtga
    35521 ctgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    35581 cgcagacggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    35641 cgtggcgtaa atacgcgttg atggcgttgg caatcatcct tttcggctgg atggcaagcg
    35701 ttgcgccaaa agagttcctt ggacacttca ctgtgttcgc gctggcctgc gttgtcggtt
    35761 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gtcaccaacg
    35821 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccagggc ggctgggtta
    35881 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    35941 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    36001 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccgggctt
    36061 tcgaaacatg aaacgtctcg ccagggtaac aatttcggta tcgccggtat ggcgattgcg
    36121 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    36181 gttattggtg gggcaattgg tatccgtctg gcgaagaaag ttgaaatgac cgaaatgcca
    36241 gaactggtgg cgatcctgca tagcttcgtg ggtctggcgg cagtgctggt tggttttaac
    36301 agctatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    36361 gtgttcctcg gtatcttcat cggggcggta acgtttacgg gttcggtggt ggcgttcggc
    36421 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    36481 ctggcggctc tgatcgtttc cttcctgctg ctgattgtat ttgttcgcac ggatagcgtc
    36541 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    36601 ttagtcgcct ccatcggtgg tgcagatatg ccagtggtgg tgtcgatgct gaactcgtac
    36661 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    36721 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    36781 cgttccttta tcagcgttat tgcgggcggt ttcggcaccg acggctcttc tactggtgat
    36841 gatcaggaag tgggcgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    36901 aattcccatt cagtgatcat tactccgggg tacggcatgg cagttgcgca ggcgcaatat
    36961 cctgtcgctg aaattactga gaaattgcgc gctcgtggta ttaacgtgcg tttcggtatc
    37021 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    37081 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    37141 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    37201 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    37261 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    37321 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    37381 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    37441 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    37501 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    37561 tgtggaaagc cccagcccac gccccatgat gcgcggctcc agaatattgc cgaagacaag
    37621 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    37681 tacctgagcg atagggggga ttgccgcgag aactgaacca atattgggga tgtaattaag
    37741 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    37801 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagacac
    37861 gctgtcaatc gcgcgctgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    37921 aaattttccg ggcaattgtg gcacttcgag caacataaac agcaccgtca gcagcaataa
    37981 aaagattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    38041 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acatcaatcc ctacgcgttg
    38101 caacaacggt tcaatcgctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    38161 ccgcgtcaac tcattgagcg cggaccccag ataagctaac agcaacacca tcgccatcac
    38221 gatgatggtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatgtg
    38281 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgataat
    38341 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    38401 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt tttcttcctt
    38461 tgctaataat aatgtctttt tcgctccttc atcataacga aaatcaaacc gctcaggtaa
    38521 agaagtgaaa atattttgag ttaattctta agctatgata caaatcaggc gtgttcaact
    38581 accgaggaca attatcatcc gcgatgacga gaagcaacac tgcggataat tgtaatatta
    38641 tggacaattt gttcaggacg ttattttcta catttaccca tcttcgtacc tcatccacta
    38701 tcttgctcgt aggtgagcag cactggcgca acgcgcttta gtcctttctt ctgcctaagc
    38761 ttgcgctatg cgcggggctt gatcacttta ctttggtttc gctgaattaa gcgaaaatta
    38821 aaataattct cttgcaggag aaggacaatg tatatttatt ggattttatt aggcctggct
    38881 attgctacgg aaattaccgg tacgctgtca atgaaatggg cgagcgtcag tgagggaaat
    38941 ggcggcttta ttttaatgct ggtgatgatt tctctgtcgt atatatttct ctctttcgcc
    39001 gttaaaaaaa tcgccttagg cgtagcttat gcgctgtggg aaggtattgg tattttattt
    39061 attaccttgt ttagcgtttt gttattcgac gaaagtttat cgctgatgaa aattgccggg
    39121 ttaaccaccc tggtggccgg gattgtgttg atcaagtcag gtacccgtaa agcgcgtaaa
    39181 cctgaactgg aggtgaacca tggcgcagtt tgaatgggtt cacgccgcct ggctggcatt
    39241 ggcaatcgtg ctggaaatcg ttgctaacgt ctttttgaaa ttttctgacg gctttcgtcg
    39301 caaaatattt ggcttgctct ctctggcggc ggtgctggct gcctttagtg cgctttctca
    39361 ggccgttaaa gggatcgact tgtctgtcgc ttatgcattg tggggcgggt ttggtattgc
    39421 cgccacgtta gccgcaggtt ggatcttgtt tggtcaacgg ttaaatcgta aaggctggat
    39481 tggcctggtg ctgctgttgg ctggaatgat catggtgaaa cttgcctgat gaacacgctg
    39541 cccgcgctgt cgggcagcgt ttgaacatta tttttgcgac agttgatcca gcttgtcgcg
    39601 aaaaccggta acagaaatgg cccggttatc ggcgcgccag cgatctttcg cggcaggagc
    39661 cgagctttgc accccaatta attgccagcc gtcatcggta tgcaacatca gaggcgaacc
    39721 gctgtcaccc ggcaaggtat cgcactgatg cgacatcacc gacgtttgcg cccagccagt
    39781 cacttcacag ttttgatgac tgtacaacgt atcgagatga tcttcagggt agcccgcctg
    39841 agtcacttta cgaccagccg cttttaatgc ggcggtaagc gcggctttat ctccctcaaa
    39901 taacggcaac ggtgtaatgc cagaaggggg attacgtagc acaatcaaac cgaagtccca
    39961 cggcgcagct gcgggaggta caatccagcc atccccatct gcttttaacc gctttcccag
    40021 tgttggatca acgcggcctt ctatgtcgtg gatctcatag cgccaaagac ctttatttga
    40081 cacgaaacgc agcgctaccg ctttatcggc tttacccttt ggcggcgtca ataaacagtg
    40141 tcctgccgtt aatgccagat tgggtgcaat cagcgtcgcc gtacataaat tgccgctggc
    40201 cgtttccagt tggccaaccg catcccacgg tgattgggtc gtgtcattca ctggcacacg
    40261 atcatcatga ccaaaaaaca gggtgctgac ctcatcgttt gccgatttgg caacgtctgg
    40321 tttatctgca aacacaaaag cagacgtcaa actaattgca cccaacacta cagcaatggt
    40381 tgtacgcata tcacactctg gtgggtaatt atgattatta aaagcaaacc ctcataaata
    40441 ctatagacgg gacgggatga aagtgggagt aaaatcagat aactacgatc aggaaagata
    40501 aaaacgggcg gcaatcagtg cgcataaaat aaggatgata agaatgaact caaagcgata
    40561 acggcccacc atactaccct ccgctaaagg cggtgctaaa cgttacccgc ttagcaccgt
    40621 aattttcagg cgcgaggggg cgcgccagca ttactgttga aaacttacgc tgcgggttgt
    40681 gcagcaggtt ttgccggttg ctgatggctg tgtttcttgg cgtgtttctt cgctgcctgc
    40741 gctttttgtt caggagcttt ctgttcagct ttggcatttt tatgatgctt tttagccgcc
    40801 tgcgctttct gggcaggtgc tgctttatgc tgttttttat gatgtgtggt tttcgcaggc
    40861 gctgctttgg tggtcgtcgc agtcggagca ggtgtggtcg cagtctctgc agcaaaggca
    40921 gcagaagaca gacccatagc agcggcaaca accagagcta atactttttt cattgtcata
    40981 ccctcaattt gttttttcat ttaaccccac tgcggggccg ttgaaataac tatatccctg
    41041 ggaatggcag acttccgtga gtggttggtt tcagcgtgta acgatatgta caaacgctga
    41101 ataaattaca gcattgataa taacgtattg tgacttaagg aaaatttagc taccaataaa
    41161 gtagtcttga tcgtgttaac tattttacta cttacaaagc ggtagaataa ctgcgcatca
    41221 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    41281 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    41341 ggtttgagca ggcaataagt ccggacgggt atttacctca gtccggactt attttttcag
    41401 gcgtgcagac gatgatgcaa acgcgtcccg accagcagag caatgaccag catcagagca
    41461 ataaatgcgc cgacgccgtt ccagccatag ttatgccaga aaacaccacc cagcgtcccg
    41521 gcaatactcg accccagata gtaactgaac agatacagcg aggaggcctg gcctttagcg
    41581 cgttttgcgc gggggccgat ccagctgctg gcaacggagt gggctgcgaa gaatccggct
    41641 gagaagagta acattccggc aaagatcagc cacagcgagc tgaataaggt catcagtaaa
    41701 ccaaacagca taacccccgt cgaaaacaac atcaccggac cgcgtccata gcgggcggtc
    41761 atggttccgg ctttgggtga gctccatgta ccggtcaaat aagccagcga taataagcca
    41821 accacggcct gactgacatg ccagggggaa agcatcaacc tatagccgat gtaattaaac
    41881 agcgtgacga acgaccccat cagcaaaaag ccttctgcga acaataacgg taatccccgg
    41941 tcacgccagt gcagacgaaa gttgataaat aacgtcttag ggcgcagcga agtcgggcga
    42001 aaatggcgtg attcagggag gattttccag aacatcaacg ccgaggccag cgcgaaacaa
    42061 ccgattgccg ccagagcaat tcgccagtta aaaaagtccg tgaagacacc gctaattaag
    42121 cgtccgctca tgccgccaat tgagttgccg ctgatataca accccattga aaaggccacg
    42181 aaactagggt ggatttcctc gctaagataa gtcatgccaa cggctgccac gccacttaac
    42241 gaaagcccaa tcaaggcgcg cataatcaaa atgccgtgcc agctggtcat cattgtcgaa
    42301 agtaacgtac aaatggaggc caacagtagc gccgtgacca tcactggttt gcgaccaatg
    42361 gcatcggata gcgggccagt aaacagcaaa ccgatagcca acatcgccgt tgaaatggac
    42421 agtgaaatac tactgttcgc aggcgttaaa ccaaactcct gcgaaagcac cggaaggata
    42481 ggttgcacac aatagagaag tgcgaaggtt gccagtccgg cagagaaaag cgccagggtg
    42541 acgcgcataa attgcggcgt accgcgttta ataaaatgat ttggctgaga aatgctttgc
    42601 ttgtcagtgt cgcttgccgg agcgccatca acagttgtag tacggctcac ttgaaatcct
    42661 tgctaaatat gcctgtggat caggcttatg aatagattag gaaaatcgaa ttgttctgtc
    42721 taatatatta ataatctcaa ataagatgtt ttaaatatga atattgaact tcgtcatctg
    42781 cgttactttg ttgctgttgc agaagagctg catttcgggc gcgccgctgc ccgcctgaat
    42841 atttcgcaac caccgctaag tcagcagatt caggcgctgg agcaacaaat tggtgcccga
    42901 ctgctggcac ggaccaatcg cagtgtattg ctgacggaag caggaaaaca gtttcttgta
    42961 gatagtcggc aaatcctgtc tatggtggat gatgctgccg ctcgcgctga aaggctgcat
    43021 cagggcgaag cgggggagtt gcgcattggt tttacttcgt ccgctccttt tattcgggcg
    43081 gtgtccgata cgttatcgct gtttcgccgt gattatcccg atgtccattt acaaacccgc
    43141 gaaatgaaca ctcgcgagca aatcgctccg ctcattgaag gaacgctgga tatgggattg
    43201 ctgcgtaaca cagcgttacc ggagacgctt gaacacgcag tcatcgtcca tgaaccgctt
    43261 atggcgatga tcccgcacga tcatcccctg gcaaataacc cgagtgtaac gctggctgaa
    43321 ctggcgaaag aaccctttgt cttttttgat ccgcacgtcg gaacagggct gtatgacgat
    43381 attctcgggc tgatgagacg ttacaatttg acgcccgtca tcactcagga ggtgggtgag
    43441 gcaatgacca tcatcggtct ggtttccgct ggtctgggtg tttcaatttt gcctgcgtca
    43501 tttaaacgtg ttcagctcaa cgaaatgcgc tgggtgccga ttgctgaaga ggatgcggtt
    43561 tctgaaatgt ggttggtctg gccgaaacat catgaacaaa gtccggctgc gcgtaacttt
    43621 cgtattcatc tgctgaatgc tctcaggtga gggaaatttc agcgaaaaag cccgaaaaat
    43681 gtgctgttaa tcacatgcct aagtaaaaat ttgacgacac gtattgaagt gcttcaccat
    43741 agcctacaga ttatttcgga gcgcgaaaat atagggagta tgcggtggtt gctgaaaacc
    43801 agcctgggca cattgatcaa ataaagcaaa ccaacgcggg cgcagtttat cgcctgattg
    43861 atcagcttgg tccagtctcg cgtatcgatc tttcccgtct ggcgcaactg gctcctgcca
    43921 gtatcactaa aattgtccgt gagatgctcg aagcacacct ggtgcaagag ctggaaatca
    43981 aagaagcggg gaaccgtggc cgtccggcag tggggctggt ggttgaaact gaagcctggc
    44041 actatctttc tctgcgcatt agtcgcgggg agattttcct tgctttgcgc gatctgagca
    44101 gcaaactggt agtggaggag tcgcaggaac tggcgttaaa agatgactcg ccattgctgg
    44161 atcgtatcat ttcccatatc gatcagtttt ttatccgcca ccagaaaaaa cttgagcgtc
    44221 tgacttcgat tgccataacc ttgccgggaa ttattgatac ggaaaatggt attgtacatc
    44281 gcatgccgtt ctacgaggat gtaaaagaga tgccgctcgg cgaggcgctg gagcagcata
    44341 ccggcgtacc ggtttatatt cagcatgata tcagcgcatg gacgatggct gaggccttgt
    44401 ttggtgcctc acgcggggcg cgcgatgtga ttcaggtggt tatcgatcac aacgtggggg
    44461 ctggcgtcat taccgatggt catctgctac acgcaggcag cagtagcctc gtggaaatag
    44521 gccacacaca ggtcgacccg tatgggaaac gctgttattg cgggaatcac ggttgcctcg
    44581 aaaccatcgc cagtgtggac agtattcttg agctggcaca gctgcgtctc aatcaatcca
    44641 tgagctcgat gttacatgga caaccgttaa ccgtggactc attgtgtcag gcggcattgc
    44701 gcggcgattt actggcaaaa gacatcatta ccggggtagg cgcgcatgtc ggtcgcattc
    44761 ttgccatcat ggtgaattta tttaacccac aaaaaatact gattggctca ccgttaagta
    44821 aagcggcaga tatcctcttc ccggtcatct cggacagcat ccgtcagcag gcccttcctg
    44881 cgtatagtca gcacattagc gttgagagta ctcagttttc taaccagggc acgatggcag
    44941 gcgctgcgct ggtaaaagac gcgatgtata acggttcttt gttgattcgt ctgttgcagg
    45001 gttaacattt tttaactgtt ctaccaaaat ttgcgctatc tcaatttggg ccaggaaagc
    45061 ataacttaga ctttcaaggt taattatttt cctggtttat atttgtgaag cataacggtg
    45121 gagttagtga tgctgaagcg tttctttatt accggtacag acacttctgt agggaaaacg
    45181 gtggtttccc gcgcattgct acaagcgtta gcctcccagg gaaaaacggt tgcgggatat
    45241 aaacccgtag cgaaggggag caaagagaca cccgaagggc tgcgtaataa agatgccctg
    45301 gtgttgcaga gtgtttcaac catcgaactg ccttatgaag cagttaatcc tatcgcgtta
    45361 agcgaagaag aaagtagcgt ggcgcacagt tgcccaatca attacaccct catttcaaac
    45421 ggcctggcaa acctgaccga taaagtcgat catgtcgtgg tagaagggac tggcggctgg
    45481 cgcagtctga tgaatgattt gcgtccgctc tctgaatggg tggtgcagga acaactgccg
    45541 gtgttgatgg ttgtcggtat tcaggaaggt tgcattaacc atgcactgct aacagctcag
    45601 gcgattgcca acgacgggct gccgctcatt ggctgggtgg ctaaccgaat caacccagga
    45661 ctggcgcatt atgcggaaat cattgatgtg ctcggtaaaa aacttccggc accgctcatt
    45721 ggtgaactgc cttatctgcc gcgcgccgaa cagcgtgaac tggggcaata catccgctta
    45781 gctatgcttc gcagtgtgct ggcggtagat agagtcacgg tgtaacgtcc gcgaaattac
    45841 cgacgcaatt acgcaggcaa tcaataaacc ggggagtagc tgatactccc cggtcatttc
    45901 acatatcatc aacgtcgaca tagtcggcgc gtgcgtggtc gccgccaaca gtgtcgccat
    45961 cccggtcaat ccgagtaaaa gcgtaatttc ttcgccatca ggtaaccata atcccaggct
    46021 acgaccatac aacatgccaa tggcaagacc gataaatagc gtcggtgtaa agacaccacc
    46081 gggcgcgcca gaaccactac tcgccagcac ggcacacagt ttacagagga agatcccggc
    46141 aatgatcatt aacagtggcg gagcggttaa aaaggattgt acggtgctat agccgttgcc
    46201 ccacactgca ggtgtgaaca gggaaagaag acccacgatc aacccgccca gtgccagttg
    46261 ccagggcggc gcaagcttga gactcacaaa tccacgatga caggcgttca ttaacgttaa
    46321 caacagtggt ccgcacagac ctgccagcac acctgtactg ataatcagcg catagtcacg
    46381 agcctgaacc gtcactgaaa gttgtacgct gtagagtaat gcgtcgctat gattaataag
    46441 attgctaacc agcaacgcca cgacggcgga aataatcacc gggccgagag aggccaacat
    46501 catagtgcca aacagcactt cggctataaa taaactacca gcaagcgggg cgcgataggc
    46561 cgcagccatt cccgccgcag ctccacaggc gatccataat ttccactcct ggcgtggcgt
    46621 aaaacgttgg gcaaaacacg aggctgcaag ggcagctaaa agaatcatcg caccttcgcg
    46681 accaattgca ctgccgctgg ttactaccag cagagaggca agcgatttaa ccagacttgc
    46741 tgcgtaatca aactgaccat cggtttgcaa cgcttccatg taatcggtcg gcgcatgagg
    46801 gcgttgctgg gtaaatttct gccagcccat cagcaacaaa cccgccgcca gtccgccgag
    46861 cgccggggtt agcagccgtc gccacgggga aagatttgtc gctgcattga ccagactgcc
    46921 agagtcatta ttgaggaaca gccactccag tagcagcatc gcatgacgaa acccggcaac
    46981 ggcaaaggcc gcaagaatac cgacgattgt tgcgataagc agacggcgga acatagtgcg
    47041 cagatccgga tagatgtgta gatgatgcat gagcagaaat gggcaatgaa acacgggcga
    47101 ctattttgcg gcaaaagagg ccagccgcaa aagcagtgtg tgctttaaga gataaaaaaa
    47161 acgtgacact gtaacctatt aattgccgaa ttgcgtgtaa tcgtcaccat ccggcaataa
    47221 tacggtgatc ctatcgaaac agcggtttaa ccgcgacagg aatcaacagt tgtgattgcc
    47281 attgcgccag cgttaagcga gcgagttcac ccagtgcacg gtagaaaggg tgttctgctt
    47341 tttcgataaa aacgtcaaga aaacgtgttg accacggaaa aaggtgccat gccagcaatt
    47401 cttcgcattc tgtctggcga ccattctccg ccaaccacgc agccatcagc agcagtgaac
    47461 caaaattatc ttccggttcg ttttgcttca tttcgaactg aatgcctttc tcgcgcatcc
    47521 actgacgaag tgccaatgtt gaatcgccaa acagcacaga ttcgcgatcc agccagaccg
    47581 atccccacgg cggagacggc agtgcccacg ggccgacaaa caaacgctgc caggcctggg
    47641 cgtgagtctc ttcacattgt gtctgaaaag ccgtcaccag cggcgctaat gacgcttctg
    47701 gtagaggcca ctgagtttcc cagccgtcat tggtgagtac cgcaaccaga ggtgcggctt
    47761 ccgcactctc tggagcgtaa taaaacagcg cgcccaacac gcgcgccgca acagaaaaat
    47821 tatcttgctg tgaaaaatgg gtcattccta acatcctgaa atgtgcgggt tgccccgcac
    47881 caatttgtta acctgcaata gccattccta ccgtcatgtg caggccataa aagagcacac
    47941 gcccgatcat ctcaccgcca agaatgagga ttagccccag aacaagtccg gcaacgtgag
    48001 gttcacggcg acgaatgagc ggacaaagcc agcatccaag acctgcgcat aacaatacca
    48061 cacgccagac ctgtaaggag gcgtaatctg gtaccagtgc gctggcttgt tgtacggatg
    48121 agtgaataga cgccagagac aaaccttgca gcacaatcaa cccggcacaa gcgatcagcg
    48181 ccagaatgct aatgatggca aatggtgtgg tattaaatgt aacgcgggct gcacgcagaa
    48241 ttgtggcagc aagtattgga ccgctcaaca ataccgtcag gaagaaagcc agcgtagtgt
    48301 aaccgttatg ccaggttggc acagtgtcga tttgatacac gcaggtcatc atccagacga
    48361 aaatgatgcc gagtgccatg ctgacaagta accagagttt ccccaacgct tgcggcattt
    48421 taccgatgac cgccaccagc caccataatc cgccaacggc aaaaaatatg gaaccggcag
    48481 caatctcgtt actcagacca gaagctccga ttctattcag cgagttaaac gcccgcagtg
    48541 gcgatcccag gtgcatgaca gaggcaataa acccgatgcc catcaacagc cagaggaaaa
    48601 acatgccacg aataatgtgc tgacggtctg cgtcattttt ggcggcgaac cagccaattc
    48661 cgctaacaat cagcgcgcct actacgcatt gacccaatac ggtaaagata accagtggcc
    48721 attcatgcca tccatttccc attttacact tcctccggat tagccagata accagtggta
    48781 tcgccggtcg ggcggctgtt ggcgttaggt ttgataacga tattgggttt tgtgaaatgc
    48841 gcgcgcggca gtggcgcgac ggcagccaga gtgccgtgct tctggcgcaa ttcttcaatt
    48901 ggaccgaact caagtgcacg cagtgggcag gattcgacac atatgggttg tttcccctcg
    48961 gcgacgcgcg aataacaacc atcgcacttc gtcatgtgcc ctttttcagc attgtactgt
    49021 ggcgcgccgt acggacaagc catgtggcag tagcggcagc cgatacaaac atcttcatcg
    49081 accaccacaa agccatcttc tcgtttgtgc attgcgccgc tggggcagac tttggtacac
    49141 gccggatcat cgcagtggtt gcacgaaatt gagagatagt aggcaaagac attctgatgc
    49201 cagacgccat tatcttcctg ccagtcgccg ccagcatatt cataaatacg gcggaaactg
    49261 acttccgggc caagatcttt aaaatctttg cacgccagtt cgcaggtttt acagccagta
    49321 cagcggctgg aatcgataaa aaatccatat tgtgtggtca tgggctactc cttaaacctt
    49381 ttcgatctgg acaagattgc tgtgcgacgg gtttcccttt gccagcggtg aagggcggtg
    49441 agaggtcaga ataccgttaa ctgcagtgga taggtccccc gtaggggatc gttccaaccg
    49501 tcgaaacctg gggtatacgc tggcagtgga tgaataattt catctttttt caattcccag
    49561 gtatccgcaa ttgtcgccag tcgttcagaa taaatttcga tttttcccga aggtgttttc
    49621 aacgggtttg cctgtggatc tacacggaat gcgcggaaag cgacgtagtg ttcttccggg
    49681 cattttttct taaagatccc ggtcgttttc atctcctcgt agtcgggcat ctcagggtta
    49741 cgttccttcg ttttcgcatg gagatatttg atccattcat gctgactgcg accttcagta
    49801 aaggtttgat aaacgtctgg tcctaagcgt ttggcgactt cactcagcat ccagtagatg
    49861 ggtttgcgtt caaattttgc tgaggttgcg ggttgggcga ggatcacata gcccatattc
    49921 ccggcagatt catgagagat aaggtcttct tgctctgttg gcatcaggtc gggtaacagg
    49981 atatcgcaat acttagccga ggcagtcatg aagtggtcaa tgccaacaat catctcgcac
    50041 ttgctgtcat cctgaagcac ctcatgggtg tgattgatgt cgccatgctg attgatcaat
    50101 gtgttactgg cgtagcacca taaaaacttg atggggacat ccagtttttc ttttcctcga
    50161 acaccatcac gggtcgcggt catttccgta ccatggtcga tggcatctgt ccatgtaaag
    50221 acggaaagcc gctcatccgc gtttctgacg ggttatttcc gaacataacg accagtttcg
    50281 tattggcgat atcatccggg ctgttgccat cattggcacc gaacatataa ctcattgcgg
    50341 cactgatctg tgcggtactg tagctgccat agcgactgag aaaaccaccg caagagttca
    50401 tcagacggta cgggacgttt gagttggtga tgtttccgcc atctacgcct gttccataga
    50461 gaacatgtac agcctcattg ccgtaatctt ttaggatccg ccgaagatta tcactgatgg
    50521 tatccagggc ttcgtcccaa cttatccgtt caaatttacc ttcaccgcgc ttgccgacgc
    50581 gcttcatggg atatttcaac ctgtcaggat gattcatccg tcggcggata gagcgcccgc
    50641 gtaaacatgc ccgaacctga tgattaccgt aaacgtcgtc acctgtcgta tcagactcca
    50701 cccagtacac ggtgtcatct ttcacatgca aacgtaacag acagcggctc ccgcagttaa
    50761 cggtgcagga actccagacc gctttctctt ctaccggtgc ctgtgccgct cggaccattt
    50821 gggaaaacgg caaagtgaaa gcactgcttg ccagcgcgag actgccaagt gcggaggttt
    50881 tcatcagact tctacgactg atttcagccg acatgagcgc ctctgtgtta tggattttca
    50941 tcattactca cttattgctt ttcaaacaaa atgtcatgtc aaaatttatg gccgtagtgg
    51001 gttatatttt ttcgatctcg accagattag tgtgctgcgg gtttcccttc gccagtggtg
    51061 aagggcgcag agtggttagc gtattcacac agccgccgtg gtcgatttta tcgccagaca
    51121 tattggcctc gtgccaggct ccctggccca tagcactaac tccagggaga atgcgtggtg
    51181 tcactttggc tggtagccga acttcgccac gatggttaaa cacccgcacc atatcgccgt
    51241 tggcaatccc acgtttctgt gcatctatag ggttgatcca cacttcctga cggcaggctg
    51301 ccttcaggac atcaatgttg ccgtaggtcg agtgcgtacg ggatttgtaa tggaaaccaa
    51361 acagttgcag tgggaaggta ctacgttcag gggagtccca cccttcaaag gttgaggcat
    51421 aaaccggcag tgggcttatc acttcatctt tttccagttc ccaggtacgg gcaatttccg
    51481 ccagcttgct ggaataaatt tcaatcttac cggaaggcgt tttaagtgga tttgcctcgg
    51541 ggtcgtcacg aaatgctttg taggcgacaa aatgaccatt gggatcttta cgcttataga
    51601 tacccatttt ttttaattcg tcgtaagacg gtaacgccgg atctttggca agcattttgg
    51661 cgtacagatg ttgtaaccat tgttcttgcg tgcgaccttc agtgaacttt tgatagacgt
    51721 caggtccaag acgtttcgcg acttcactca ggatccagta aattggtttg cgttcgaatt
    51781 tttcgctggt gacaggctgg aggaaaatga gatatcccat gttaccggcg tagtcgttag
    51841 gaataatatc ttcctgctca acggtcatta ggtctggcag cagaatgtcg gcatattttg
    51901 ccgatgaggt cataaagttt tcgatgacca caatcatttc gcatttcgat tcgtcctgca
    51961 gaatttcatg cgttttgttg atgtcagaat gctgattaac cagggtattt cccgcgtagt
    52021 tccagatgaa cttaatgggc acatctagtt tatctttgcc ccggacgccg tcgcggattg
    52081 ccgtcatttg cgggccatga tcgatagcat ctgtccaact gaagcaggag attgacgttt
    52141 tgaccggatt atccagcacc ggcaggcgtt cgatggtaat ggtgtaggtc gattcacgcg
    52201 caccactatt tccgccgctg atgccgacat tgcccgtcaa aataggcaac atagcaatag
    52261 cgcgtgccgt cagctcgccg tttgcctgcc gttgcggccc ccagccctgg cagatataag
    52321 cgggttttgc tgtgccaatt tcacgcgcca gtttgatgat acggtctacc gggatacctg
    52381 taatttgcga agcccactgc ggcgttttcg ctgttttatc atcaccttca ccaagaatat
    52441 aggctttata gtgaccattt ttgggtgcat ctgcgggtaa ggttttttcg tcatagccga
    52501 cgcagtattt atcgagaaaa ggttgatcaa cgagattttc gttaatcaat acccaggcaa
    52561 tacccgcaac cagcgcggca tcggtgcccg ggcgaatagg aagccattca tcttcacgac
    52621 cagcagccgt atcggtatat cgcggatcga taacaatcat ttttgcgttc gatttctcgc
    52681 gcgctttttc aagaagataa gtgatgccac cgccgctcat gcgggtttca gccgggttgt
    52741 taccaaacat cacgaccagc ttgctgtttt caatatccgt ggtgctgttg ccatcattac
    52801 tgccgtaggt gtagggcatg gcacaggaaa tttgcgcagt gctgtaggag ccatactggt
    52861 ttagtgaacc gccgtagcag ttcatcaggc gtttgaccgc cgaggctgat ggagaagagc
    52921 gggtcatatt gccgccaaca atccccgaag agtactgaat atatacagcc tcattgccat
    52981 attgttcgac ggtttttttc aggctactgg cgatagtatc cagggcttca tcccagctaa
    53041 tccgttcgaa tttgccttcg ccgcgtttgc ccacgcgttt cattgggtaa ttcaggcgat
    53101 cgggatgatt aatacgccga cggatggagc gaccgcgcaa acaggcgcgt acctgatggt
    53161 tgccatactt gtcgctgccg gtgttgtcag tttccaccca ggtcacttca ttatctttaa
    53221 catgcagacg aagtgcacag cggctaccac agttgacgga acaggcaccc cagaccactt
    53281 tttcgctggc ctgttgtacc gctgctgctg cactgcgcag ggtaaacggc aaagaaaaac
    53341 cgcctgcagc cagcgccaga gaacctatcg cagtagattt aacgagtgtt ctgcggctga
    53401 tgcccaccat ttgttcattt ttggacataa ctcactccct gttctttatc gttatataaa
    53461 tgtttatata ttgaatattt agcgcgctaa caatagaggg aatctaccca tttggggtta
    53521 ataattatta atccatatca atagaagggt atgagtaata aggtggggtt atgttgaatg
    53581 ttcaaatcgc cggatgtgtc gcatccggcg ttcagtcgat aatgtgttac tgcggttcgg
    53641 caggcgcgcc atcctgggta gactgcgcgg gagcagagac gttaccgctg gtggtacggg
    53701 tgtagagaat tttatgcgta tcattagcgc aatgcccgac gacctgggaa tcaggctgat
    53761 caacctggtc attgggtaca atacttaacg tgaagctgct ttcgggtacg ccattattga
    53821 taatgcgctg tgatatatcg ctctgtatgc gctcacagga tcccggcgcg gcgagtaccg
    53881 tgggtgaggc gagggcgagc agaagcgcgg cacagtaggt tgagtgtttc atcataagct
    53941 ccttacgcga agataacttc tttaagcata gcatttaacg tgtaaagtac tgtatttgct
    54001 actatgattg agaatcatct ctactctcag gtgactgttg tgaaatacaa attactgcca
    54061 tgcttactcg cgatattact cacaggatgt gaccgcacag aggtaacact ttcatttacc
    54121 ccggagatgg ccagtttctc taatgaattc gattttgatc cgctgcgtgg tcccgtaaaa
    54181 gatttcactc agacattaat ggatgagcaa ggtgaagtga cgaaacgtgt ttcggggact
    54241 ttgtcggaag aaggctgttt tgattctctc gaattactgg atctggaaaa taataccttg
    54301 gttgccctgg tactggatgc caattattac cgtgatgccg agacgctgga gaaaagagtt
    54361 cgtttgcagg gaaaatgcca gctagcagaa ttaccttctg ccggggtgag ttgggaaacc
    54421 gatgataatg gtttcgtgat taaagccagc agcaaacaga tgcagatgga atatcgctat
    54481 gatgatcagg ggtatccgct gggtaaaacc acgaaaagta acgataaaac attatctgtc
    54541 agcgccacgc catcaacgga cccgatcaaa aaattagatt acactgctgt tactttactg
    54601 aataatcaac gggttggtaa tgtaaaacag agctgtgaat atgacaatca cgccaatccg
    54661 gtggactgtc agctaatcat tgttgatgaa ggagtaaaac ccgccgtcga acgggtttat
    54721 accatcaaaa atacgatcga ttattattaa tactattgtg cggtcggctt caggagagtc
    54781 tgacccggtg ttttgtgctc agccagatac tgatgctgga atatacacat gcgaatggca
    54841 ttacgatatt gaccattaat aaagaactca tgcatcaatt caccttcaac cgtaaagcca
    54901 agcttgcggt aaatgtgaat cgctttttca ttctctttat caacgatcag atacagctta
    54961 tagagattga gaacggtgaa gccatagtcc attgctaatt tggcggcacg ggttgccaga
    55021 cctttcccct gatactccgg ggagataatt atctgaaatt ctgcgcggcg atgaacatgg
    55081 ttaatttcca ccagctccac cagaccggct ttttcgccgt cacattccac cacaaagcgc
    55141 cgttcgctct gatcgtgaat atgcttatca tacagatcag agagttcaac aaaggcttcg
    55201 tagggttcct caaaccagta acgcatcaca ctggcgttat tgtcgagttg atgtacatag
    55261 cgtaaatctt cacgctccag cgggcgtagc ttaacactgt gggcgcttgg cataacgtgt
    55321 ccttacattc cttaaatcaa taacaggtta ggggataata acgcggccag ttcgacggtc
    55381 caggcagcgc aaagtatttg gctcccagta ggcattgatg ttggcgcttt gctcacattt
    55441 atcgcggtta tcaaaagcag cgtcggcttt atcccactct ttttcagtgc gtttattcac
    55501 tttctggcgc agattacgcg tgtcattcca ttgctctttt tccatagcgg cgcgctggcg
    55561 gctttgtgca ctgtcgccag actcaatcac cagtttgtta gtttcggcat gaacagttgt
    55621 gctcaatacc agtgcgcaag gcagcagaaa agcgagcagg ccgattcgtt tgctgagagt
    55681 gattttcata attcattccc tgtatgaatg attaaagctg attctacacc atccactgcg
    55741 aacgcaaaac gttcaaggag ggtgtttata ttgatgatat tatgtcgccc tataactata
    55801 catgatgtca ataagtgaca aagatgatta aaacaacgtt actatttttt gctactgcgc
    55861 tgtgtgaaat tattggatgc tttctgccct ggttgtggtt aaaacgaaac gccagtatct
    55921 ggctgttgct tccggcgggg atttcactgg cactgtttgt ctggttgtta acgttgcatc
    55981 ctgcggcgag tgggcgtgtt tacgcggctt atggtggcgt ttatgtttgc acggcgttga
    56041 tgtggctgcg cgttgtggat ggcgtgaaac tgactcttta tgactggacg ggggcgttaa
    56101 ttgcgctttg cggcatgttg atcattgttg cgggctgggg gcgcacgtag gaacataaat
    56161 ccattttatc aataagatac gaggaagtgt cagctgacaa aaggtattct atttcatctt
    56221 ttgtcaacca ttcacggcgc aaatatacgc ctttttttgt gatcactccg gctttttttg
    56281 atcttcatac ttgtatggta gtagttcagt tgcgtagatt tcatgcatca cgacaagcga
    56341 tgcaaggaat cgaacatgaa gatcgtaaag gctgaagttt ttgttacctg tccggggcgt
    56401 aatttcgtca cattaaaaat caccactgag gacggtatta cgggccttgg ggatgccacc
    56461 ctaaatggac gtgagctttc cgtggcctct tatttgcagg atcacctttg tccgcagctt
    56521 attggtcgcg atgcacaccg tatcgaagat atctggcagt ttttctataa aggtgcttac
    56581 tggcgtcgcg gtccggttac gatgtcggcc atttcagcgg ttgatatggc gctgtgggat
    56641 attaaagcca aagctgccaa catgccgctt taccagttac tcggcggcgc gtctcgtgaa
    56701 ggggtgatgg tttattgcca taccaccggt cacagtattg atgaagctct ggatgattat
    56761 gcccgtcatc aggagctggg attcaaagcc atccgcgtgc agtgcggaat ccctggtatg
    56821 aaaaccacct acggcatgtc gaaaggtaaa ggtctggctt atgaacccgc aaccaaagga
    56881 cagtggccgg aagagcagct gtggtcgacg gagaaatacc tcgatttcat gccgaaattg
    56941 tttgacgcgg tacgtaacaa gtttggattt aatgaacatt tgctgcatga catgcaccat
    57001 cgcttaacgc ctattgaagc ggcgcgcttt ggtaaaagca ttgaagatta tcgcatgttc
    57061 tggatggaag acccgacgcc tgcggaaaac caggaatgct tccgtctcat tcgccaacat
    57121 accgtcacac ccatcgcggt gggtgaagtc ttcaacagca tctgggactg caaacaactg
    57181 attgaagagc aactcatcga ttatatccgc accacgctga ctcatgcagg cggaattacc
    57241 ggtatgcgtc ggattgccga ttttgcttcg ctgtatcagg tacgtactgg ctcacacggt
    57301 ccttccgatt tgtcaccagt ctgcatggct gcggcgctgc actttgatct gtgggtcccc
    57361 aatttcggtg tccaggaata catgggttat tccgaacaaa tgctcgaagt cttcccgcac
    57421 aactggactt tcgataacgg ctatatgcat ccgggagaaa aaccgggtct tggcatcgaa
    57481 ttcgatgaaa agctggcggc gaaatatccc tatgaacctg cttatctgcc agtcgcacgt
    57541 ctggaagatg gcacgctgtg gaactggtaa ggagtaaggt aatgaaaagc attttaattg
    57601 aaaaaccgaa tcaactgtcg attatcgaac gtgaaatacc caccccgtca gcgggtgaag
    57661 tacgagtaaa agtgaaactt gccggaattt gtggttcaga tagccatatt taccgtgggc
    57721 ataatccttt tgcgaaatat ccgcgcgtca ttggtcatga attctttggc gtcattgatg
    57781 cagtgggtga aggcgtggaa agcgccagag tcggtgaacg cgttgctgtc gatccggtgg
    57841 tcagctgtgg gcattgctat ccgtgctcta taggtaagcc gaacgtttgt acgacacttg
    57901 ctgtattagg tgtgcacgct gacggtggtt tcagtgaata tgccgtggtt ccggcaaaaa
    57961 atgcgtggaa aattcctgaa gcagtggccg atcaatatgc ggtgatgatc gaacctttta
    58021 ccattgcggc taacgttacc ggtcatggtc aaccgactga aaatgatacc gttctggttt
    58081 acggtgccgg tccaatcggc ctgacgatcg ttcaggtatt aaaaggcgtc tataacgtta
    58141 aaaatgtgat tgttgccgat cgcattgatg aacgactgga aaaagcgaaa gagagcgggg
    58201 cagactgggc gattaataac agccagacac cacttggcga gattttcgat gaaaaaggca
    58261 tccaaccgac attagttatc gatgccgctt gtcatccttc aatcttgaaa gaggccgtaa
    58321 cgctggcttc tccagcggca cgtattgtat tgatgggctt ctccagtgaa ccgtctgaag
    58381 tgattcagca aggaattacc ggaaaagaac tctctatttt ctcttcacgc ttaaatgcaa
    58441 ataaattccc ggtcgttatc gactggttaa gtaaagggtt aattaaacca gaaaaactaa
    58501 ttacccatac gtttgatttc cagcatgttg cggatgccat tagtttattt gaacaggatc
    58561 aaaaacattg ctgcaaagtc ttactcactt tttctgaata ataccaataa cggcgaataa
    58621 gtagtacgca tcttacctct tttttagaga taaccattat gacaatagaa aaacacgaaa
    58681 gaagcactaa ggatttggtg aaagcagcag tatcgggatg gctgggcact gcgcttgaat
    58741 ttatggattt ccagttatat tcgctcggcg cagcgttagt gtttcatgaa atattttttc
    58801 ctgaatcatc aacggcaatg gcgttaattc tggcaatggg aacctatggt gcaggttatg
    58861 tggcacgtat tgtcggagca tttattttcg gcaaaatggg cgacagaata gggcgtaaaa
    58921 aagtgctctt tattaccatc accatgatgg ggatctgtac caccttaatt ggtgtgttac
    58981 cgacttatgc acaaattggt gtttttgccc ccatcttgct ggtgacgctg cgtattattc
    59041 aggggttggg tgcaggtgcg gaaatttccg gtgccggtac gatgctggcg gaatatgcgc
    59101 caaaaggtaa gcgcggaatt atctcctcat ttgtggctat gggaactaac tgcggaacct
    59161 tgagcgcaac ggcaatctgg gcctttatgt tcttcattct cagtaaagag gaactgctgg
    59221 cgtggggatg gcgtatacct ttcctggcga gcgttgtcgt gatggtcttt gctatctggt
    59281 tgcgtatgaa tctgaaagaa agcccggtct ttgagaaggt taacgacagc aaccaaccga
    59341 cagcaaaacc tgcacctgct ggtagcatgt tccagagcaa atccttctgg ctggcaacag
    59401 ggctgcgttt tggtcaggct ggtaactcag gtttaattca gactttcctt gcaggctatt
    59461 tagtgcagac gttattgttt aacaaagcaa ttccaacaga tgcattgatg atcagttcga
    59521 ttctcggctt tatgaccatt ccgttccttg gttggttatc cgataaaatt ggtcgccgga
    59581 tcccgtatat tattatgaat acctctgcga ttgtgctggc atggccaatg ctttctatca
    59641 tcgtagataa aagctatgcc ccgagcacca ttatggttgc actgattgtg attcataact
    59701 gtgcggtgct tggattattt gctctggaaa acattaccat ggcagaaatg ttcggctgta
    59761 aaaaccgctt tacccgaatg gctatttcta aagaaattgg tggtcttatc gcttccggtt
    59821 ttggtcctat cctggcgggg attttctgca ccatgacgga atcctggtat ccgatcgcca
    59881 ttatgatcat ggcatattct gtgattggtt taatctctgc gctgaaaatg ccagaagtga
    59941 aagaccgtga tttaagtgcg ctgcaagacg ccgcagaaga tcaaccacat gttgtaagag
    60001 ctgcgcaacc gtccagaagt ctgtaaatcc tcaatccctt ctcttaccgg agaggggatt
    60061 tttattcata taaaaaaaca tgtagcttgc tatatttaat tttaaggaat tgttatggga
    60121 aatcatttgt tatcagcaaa agcgacgctc cctgtttatg atcgtaataa cctggcccca
    60181 agaattgttc atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat
    60241 attcttgcta cggaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc
    60301 gaacagcaaa ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg
    60361 gctgatgcgt ggacggctcg cgtcgttggc gtcgttaaaa aagccttaca cgtacaaata
    60421 gacggcttag aaaccgtgtt ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg
    60481 acaatcaccg aaaaagggta tttccactct ccggcgaccg gacagttaat gctcgatcac
    60541 ccgatggtcg ttgccgacgt gcaaaatccc caccagccga aaactgcaac aggggtgatt
    60601 gttgaggcgc tggctcgccg taaagcggca ggacttcccg catttaccgt catgtcatgt
    60661 gacaacatgc cagaaaacgg tcatgttatg cgtgacgttg tcacttccta tgcgcaagcc
    60721 attgatgtaa aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg
    60781 gaccgtattg tgcccgcagt gacagaggat acgctggcga aaatcgaaca acttaccggt
    60841 gtgcgcgatc cggcaggcgt tgcctgtgaa cctttccgcc agtgggtaat agaagataac
    60901 tttgttgccg gacgtccgga atgggaaaaa gcgggagtcg aactggttag tgatgtgctg
    60961 ccttatgaag agatgaagtt gcgcatgctc aatggcagtc attcattcct ggcgtatctg
    61021 ggctatcttg ccggatatca gcacattaat gactgcatgg aggatgaaca ttatcgtcat
    61081 gcggcgtata ccttaatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat
    61141 ttgcaagatt acgctaaccg attaattgaa cgctatagca acccggcgtt acgtcatcga
    61201 acctggcaga ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt
    61261 cgctggcatc tggcgcatga cagcaagttc gatctgctgg cgctgggcgt cgcgggttgg
    61321 atgcgttatg tcggtggtgt tgatgaacag ggaaatccga tagaaatcag tgacccactg
    61381 ttacctgtta ttcagaaggc agtacaaagt agtgctgaag ggacagcgcg cgtgcagtca
    61441 ttgttggcga ttaaggcgat atttggtgat gatttgccag ataatagctt gtttacgact
    61501 aaagtgacgg aagcgtactt gtctttatta gcgcatggcg cgaaagcgac agtggcgaaa
    61561 tattccgtga agtaaaaaaa gcaatgccgc catcggcggc attcacacgc ttagttcatg
    61621 ccgagacgaa tcaggtccag tggtgccagt ttctcttcac aaccttcaac aactacggtt
    61681 tttccgcggc ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca
    61741 gtgtgaccgg tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga
    61801 tcgtaggtcg tcataggtgt tttctccttt ctgatttaca gtcgaagccc acgtaacggg
    61861 cgcaatataa atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt
    61921 ttttgatgca tgcatgagcc ataagtcggg gggaaagact ctcaccccgg atgggatgag
    61981 aggaaatgaa ttactcttcg ctaaaccagt cgctgttttc ctggcggatc tggcgcacgg
    62041 attcgctgat ttcctgcaaa tgttgcgtca ttgcacgttc taccgcatcg ccatcacgtt
    62101 tttgcagggc agagaaaata tcaagatgct ggcgtaacag catttctggt ggagaaacgt
    62161 ggtcgaagct catatagcgc acgcgatcaa cggtcgcttt caggttctca atggtatccc
    62221 acgccagttg acagtcggca atctgcgtca ggagttgatg gaagttgtca tcaagttcaa
    62281 aaaaatcatc cagttgcttg cgctcaatgg caatgcgttg ctggtgaaga ttttgttcca
    62341 gttggtagca ctggctttcg gtaatcatgc tcgccgcccg acgcgccacc gcgcactcaa
    62401 tggcctgacg gataaaactg ccgttgcgca cctgggccat ggaaattttg ttgacgtagc
    62461 tgccacgctg cggacgaatt tgaatcagac cgttttccgc cagtttaata aaggcttcac
    62521 gaaccggctg gcgtgacaca ttgaaacgaa cagaaacttc tttttccgac aacggtgtgc
    62581 ctggtgcaat caggcaatgg acaatgtcgc ggcgaagaat acgataaatc tgctgattga
    62641 caggctgtgt gggattaagt tgcgtttcga cggtcattcg ttcttgcttg tgagtgagtt
    62701 aactgcatga gtctaccact tttttgtgac aagcaataac ccggcagtta cgccgggtat
    62761 aaaaattact gacggtggac attcagtccg gcatagcttt gggtaaccgg catcatttcc
    62821 agtgtattga tattgacgtg agcaggcagc gttgacaccc accagacggc ttcgctgaca
    62881 tcttctggcg tcaatgcaac ggtattttga taggtttttt ccgctttacc gtcatcgcct
    62941 ttaaagcgga cattggaaaa ctcggtgcca cccaccagac ccggttcgat gtcggtgacg
    63001 cgcaccgccg taccatgcag gtccgtacgc agattcaggc taaattgacg aacaaacgct
    63061 ttcgtcgcac cgtaaacgtt accaccggca tacggccagc tacctgccgt tgagccaatg
    63121 ttaataatat gaccatgatt acgttcaacc atacccggta agacggcgcg cgtcatatat
    63181 accaggcctt tgttgttggt atcaatcatc gtttcccagt cttcaatgct ggctttatga
    63241 gcaggctcca tgcccaacgc caggccggca ttgtttacca ggatatcaat attgctccac
    63301 tcggcaggga gcgatgccag catctcttca atagcggcgc ggttgcgaac gtccagttgg
    63361 gcgatataca gattatctcc cagttcgtct tttaactcct gcaaccgctc ctggcgacga
    63421 ccagtggcga taactttatg cccttgttga ataaaacgac gagtaatgca ttcaccaaaa
    63481 cctgccgttg ctccagttac taaaacgatc atctcactgt tcctcaacga ttttgtgtgt
    63541 caataacata gcacgtgatg cttaagagcg ggtaatactg gtttgcaaga ttgcactccg
    63601 ttatcagtaa gcctacgcta tggaacacct cgcgttcagg agaagaaaat gacaacaatg
    63661 aatcctttcc ttgtgcaaag cacactgccg tatctggctc cccattttga tcaaattgcc
    63721 aatcatcact atcgcccggc attcgatgag ggaatgcagc aaaagcgggc agaaattgct
    63781 gccatcgcgc ttaacccgca aacacctgat ttcaacaata ctattctggc actggaacaa
    63841 agcggagaat tacttacccg cgttaccagc gtcttttttg cgatgactgc ggcgcatacc
    63901 aatgatgaat tacagcgtct tgatgaacag ttttccgctg aactggcgga acttgcgaat
    63961 gatatctatc tgaacgggga attattcgcg cgggtagatg ctgtctggca acgccgtgaa
    64021 tccctggggc ttgatagtga atccatccgc ctggtggagg tgattcatca acgttttgtc
    64081 cttgccggag ccaaacttga gcaagctgat aaagcaaaat taaaagtact gaatacagaa
    64141 gctgcgaccc tgaccagcca gtttaaccag cggttactgg cagcaaataa atccggcggt
    64201 ctggttgtga acgatatcgc gcagctggca ggaatgagtg aacaagagat tgcgctggcg
    64261 gcagaggcgg ctcgcgagaa aggtctggat aacaactggc tgattccgct gctgaatacc
    64321 acccagcaac cagcgcttgc tgaactgcgc gatcgcgcga cgcgtgaaaa actgtttact
    64381 gcgggctgga ggcgagcgga aaaaaatgat gccaatgata cccgcgctat cattcaacgt
    64441 ctggtggaga tccgcgcgca gcaggcgaaa ctgcttggtt ttcctcatta tgccgcatgg
    64501 aaaatcgccg atcagatggc aaaaacgcca gaagcagcgc tcaactttat gcgggaaatt
    64561 gttccagcgg cgcgtcaacg tgcgagcgat gaattagcct ccatacaggc ggttatcgat
    64621 aagcagcagg gcgggtttag cgcgcagccg tgggactggg cattttatgc cgagcaggta
    64681 agacgtgaga aattcgatct cgatgaagcg cagctcaagc catattttga attaaacacg
    64741 gtgttgaatg aaggcgtatt ctggactgcg aatcagctct tcggtattaa gtttgtcgaa
    64801 cgttttgata ttcctgtcta ccatcctgac gttcgtgtgt gggaaatttt tgatcataat
    64861 ggcgtggggc tggcgttatt ttacggtgat ttcttcgccc gtgattcaaa aagcggcggc
    64921 gcatggatgg gcaattttgt tgagcaatca acgctgaatg aaacgcaccc ggtaatttat
    64981 aacgtctgca attatcagaa acccgctgcc ggtgagcctg cgttgttact ctgggatgat
    65041 gtaataacct tattccatga atttggtcat acgctgcacg gcctttttgc ccgccagcgt
    65101 tatgccacgc tttccggcac caacacgccg cgtgattttg tcgaatttcc gtcgcaaatc
    65161 aacgaacact gggcaacaca tccgcaggta ttcgctcgct acgcccggca ttatcagagc
    65221 gggaccgcaa tgcctgacga acttcaacag aaaatgcgta atgccagcct gttcaataaa
    65281 gggtatgaga tgagcgaact gcttagcgcc gcacttctcg atatgcgctg gcattgcctg
    65341 gaagaaaacg aagcaatgca ggatgtcgat gatttcgaat tgcgggcgct ggtggcggaa
    65401 aatatggatc ttcctgctat accgccacgc tatcgcagca gttatttcgc ccatattttt
    65461 ggtggcggat atgccgcagg ttattacgct tatctgtgga cgcaaatgtt ggccgatgat
    65521 ggttaccagt ggtttgttga gcagggcgga ttaacgcgtg aaaatgggcg gcgttttcgc
    65581 gaggcgattc tttccagagg taacagcgaa gatctggaac gcctgtaccg acaatggcgc
    65641 ggtaaggcac ctcagattat gccgatgttg caacatcgtg gcttgaacgt ataaacacca
    65701 ccaatggctg gcctggattt atcgccagac cagcctcgtt atagcagtaa ttgcagccgc
    65761 tgggcgaggg caaacttcac cgctaatgcg aatgccgttt cgcagtcgcc agcaaaatta
    65821 ctttttttgt atactcttcc gttataataa ttttcaaatt cagccggacc agatgatcaa
    65881 cattagcggc agtctggata agaaaagcgc gccggccgtc gttcgggtca ctcatttgca
    65941 gaaataaaaa taaattagcc tgatggcctg aactactggg ccattagggt attgggttat
    66001 gctatttgtc atccttttaa ttgcgata
//
LOCUS       DACADF010000006        64470 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.006, whole genome shotgun sequence.
ACCESSION   DACADF010000006 DACADF010000000
VERSION     DACADF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.006"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            3..629
                     /locus_tag="ISB30_000394"
     CDS             3..629
                     /locus_tag="ISB30_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HAP1988074.1"
                     /translation="MLFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLDH
                     YDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLFV
                     VPVSLLFTIIAVHYTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGKL
                     TDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            656..2317
                     /locus_tag="ISB30_000395"
     CDS             656..2317
                     /locus_tag="ISB30_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP1988075.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLSGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            2557..2616
                     /gene="ibsE"
                     /locus_tag="ISB30_000396"
     CDS             2557..2616
                     /gene="ibsE"
                     /locus_tag="ISB30_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAP1988076.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2736..4169)
                     /gene="hldE"
                     /locus_tag="ISB30_000397"
     CDS             complement(2736..4169)
                     /gene="hldE"
                     /locus_tag="ISB30_000397"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAP1988077.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQLDKKG"
     gene            complement(4217..7057)
                     /gene="glnE"
                     /locus_tag="ISB30_000398"
     CDS             complement(4217..7057)
                     /gene="glnE"
                     /locus_tag="ISB30_000398"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAP1988078.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     ALLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(7080..8381)
                     /locus_tag="ISB30_000399"
     CDS             complement(7080..8381)
                     /locus_tag="ISB30_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAP1988079.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     DWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8623..9243
                     /locus_tag="ISB30_000400"
     CDS             8623..9243
                     /locus_tag="ISB30_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAP1988080.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINRLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9307..10545
                     /gene="cca"
                     /locus_tag="ISB30_000401"
     CDS             9307..10545
                     /gene="cca"
                     /locus_tag="ISB30_000401"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAP1988081.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVSLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDSLFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10625..11446)
                     /gene="bacA"
                     /locus_tag="ISB30_000402"
     CDS             complement(10625..11446)
                     /gene="bacA"
                     /locus_tag="ISB30_000402"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP1988082.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGENKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11537..11905)
                     /gene="folB"
                     /locus_tag="ISB30_000403"
     CDS             complement(11537..11905)
                     /gene="folB"
                     /locus_tag="ISB30_000403"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAP1988083.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            12010..12627
                     /gene="plsY"
                     /locus_tag="ISB30_000404"
     CDS             12010..12627
                     /gene="plsY"
                     /locus_tag="ISB30_000404"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP1988084.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12640..13572)
                     /gene="ttdR"
                     /locus_tag="ISB30_000405"
                     /gene_synonym="dan"
     CDS             complement(12640..13572)
                     /gene="ttdR"
                     /locus_tag="ISB30_000405"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HAP1988085.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13779..14690
                     /gene="ttdA"
                     /locus_tag="ISB30_000406"
     CDS             13779..14690
                     /gene="ttdA"
                     /locus_tag="ISB30_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HAP1988086.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14687..15292
                     /gene="ttdB"
                     /locus_tag="ISB30_000407"
     CDS             14687..15292
                     /gene="ttdB"
                     /locus_tag="ISB30_000407"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAP1988087.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15340..16803
                     /locus_tag="ISB30_000408"
     CDS             15340..16803
                     /locus_tag="ISB30_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAP1988088.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHAMLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16846..17859)
                     /gene="tsaD"
                     /locus_tag="ISB30_000409"
     CDS             complement(16846..17859)
                     /gene="tsaD"
                     /locus_tag="ISB30_000409"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP1988089.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            18097..18312
                     /gene="rpsU"
                     /locus_tag="ISB30_000410"
     CDS             18097..18312
                     /gene="rpsU"
                     /locus_tag="ISB30_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP1988090.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18423..20168
                     /gene="dnaG"
                     /locus_tag="ISB30_000411"
     CDS             18423..20168
                     /gene="dnaG"
                     /locus_tag="ISB30_000411"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP1988091.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVAMSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20363..22204
                     /gene="rpoD"
                     /locus_tag="ISB30_000412"
     CDS             20363..22204
                     /gene="rpoD"
                     /locus_tag="ISB30_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP1988092.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22282..22788)
                     /gene="mug"
                     /locus_tag="ISB30_000413"
     CDS             complement(22282..22788)
                     /gene="mug"
                     /locus_tag="ISB30_000413"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAP1988093.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22913..22988
                     /locus_tag="ISB30_000414"
     tRNA            22913..22988
                     /locus_tag="ISB30_000414"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22946..22948,aa:Ile,seq:cat)
     gene            complement(23042..23806)
                     /gene="nfeF"
                     /locus_tag="ISB30_000415"
     CDS             complement(23042..23806)
                     /gene="nfeF"
                     /locus_tag="ISB30_000415"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HAP1988094.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGSRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAKPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFETEQYDPQRVRAAAYWHAK"
     gene            24094..24717
                     /locus_tag="ISB30_000416"
     CDS             24094..24717
                     /locus_tag="ISB30_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP1988095.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLCVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(24824..26344)
                     /locus_tag="ISB30_000417"
     CDS             complement(24824..26344)
                     /locus_tag="ISB30_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAP1988096.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFIVLAAMLWFVAAPVVTYIFCALVMLLGNACFEWQIVRPIENVASQ
                     ALKVATGERNSVEHLKRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQASAMVKHRASRLEDAVTVLH"
     gene            26651..28141
                     /gene="ygjG"
                     /locus_tag="ISB30_000418"
     CDS             26651..28141
                     /gene="ygjG"
                     /locus_tag="ISB30_000418"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAP1988097.1"
                     /translation="MITTFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            complement(28183..28515)
                     /locus_tag="ISB30_000419"
     CDS             complement(28183..28515)
                     /locus_tag="ISB30_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HAP1988098.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            28734..29717
                     /gene="ebgR"
                     /locus_tag="ISB30_000420"
     CDS             28734..29717
                     /gene="ebgR"
                     /locus_tag="ISB30_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAP1988099.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            29901..32993
                     /gene="ebgA"
                     /locus_tag="ISB30_000421"
     CDS             29901..32993
                     /gene="ebgA"
                     /locus_tag="ISB30_000421"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAP1988100.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLSL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSARF
                     DFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWENVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLCDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLPGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSQASYEFGAGFFSTNLHSENKQ
                     "
     gene            32990..33439
                     /locus_tag="ISB30_000422"
     CDS             32990..33439
                     /locus_tag="ISB30_000422"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAP1988101.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            33502..34935
                     /locus_tag="ISB30_000423"
     CDS             33502..34935
                     /locus_tag="ISB30_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP1988102.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            35069..36139
                     /gene="ygjJ"
                     /locus_tag="ISB30_000424"
     CDS             35069..36139
                     /gene="ygjJ"
                     /locus_tag="ISB30_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HAP1988103.1"
                     /translation="MKLITAPCRALLALPFCYAFSALGEEARPAEHDDTKTPEMISTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYDSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            36156..38507
                     /gene="ygjK"
                     /locus_tag="ISB30_000425"
     CDS             36156..38507
                     /gene="ygjK"
                     /locus_tag="ISB30_000425"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP1988104.1"
                     /translation="MKIKTILTPVACALLMSFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLISKDVQVEMILRFATPRTSLLETKITSDKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPMQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIIERGKGPESWSPLFNGEATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGSQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            38733..40751
                     /locus_tag="ISB30_000426"
     CDS             38733..40751
                     /locus_tag="ISB30_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAP1988105.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAEMVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(40796..41212)
                     /gene="higA"
                     /locus_tag="ISB30_000427"
     CDS             complement(40796..41212)
                     /gene="higA"
                     /locus_tag="ISB30_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAP1988106.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(41209..41523)
                     /locus_tag="ISB30_000428"
     CDS             complement(41209..41523)
                     /locus_tag="ISB30_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAP1988107.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(41808..42944)
                     /gene="rlmG"
                     /locus_tag="ISB30_000429"
     CDS             complement(41808..42944)
                     /gene="rlmG"
                     /locus_tag="ISB30_000429"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAP1988108.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            43029..43532
                     /locus_tag="ISB30_000430"
     CDS             43029..43532
                     /locus_tag="ISB30_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAP1988109.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            43609..44301
                     /locus_tag="ISB30_000431"
     CDS             43609..44301
                     /locus_tag="ISB30_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAP1988110.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNAS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDSAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKAIFCDYAGFSTLDSVVRANKVFSENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPAKE"
     gene            44380..45366
                     /locus_tag="ISB30_000432"
     CDS             44380..45366
                     /locus_tag="ISB30_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP1988111.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNQAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            45649..46614
                     /gene="alx"
                     /locus_tag="ISB30_000433"
     CDS             45649..46614
                     /gene="alx"
                     /locus_tag="ISB30_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAP1988112.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            46938..48182
                     /gene="sstT"
                     /locus_tag="ISB30_000434"
     CDS             46938..48182
                     /gene="sstT"
                     /locus_tag="ISB30_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP1988113.1"
                     /translation="MTTQHSPGLFRRLAHGSLVKQILVGLILGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(48186..48737)
                     /locus_tag="ISB30_000435"
     CDS             complement(48186..48737)
                     /locus_tag="ISB30_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAP1988114.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(48820..50307)
                     /locus_tag="ISB30_000436"
     CDS             complement(48820..50307)
                     /locus_tag="ISB30_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP1988115.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(50322..51734)
                     /gene="uxaC"
                     /locus_tag="ISB30_000437"
     CDS             complement(50322..51734)
                     /gene="uxaC"
                     /locus_tag="ISB30_000437"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP1988116.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            52217..53515
                     /locus_tag="ISB30_000438"
     CDS             52217..53515
                     /locus_tag="ISB30_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1988117.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            53645..54421
                     /gene="exuR"
                     /locus_tag="ISB30_000439"
     CDS             53645..54421
                     /gene="exuR"
                     /locus_tag="ISB30_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAP1988118.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            54766..55428
                     /locus_tag="ISB30_000440"
     CDS             54766..55428
                     /locus_tag="ISB30_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP1988119.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKFEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            55432..55815
                     /gene="mzrA"
                     /locus_tag="ISB30_000441"
     CDS             55432..55815
                     /gene="mzrA"
                     /locus_tag="ISB30_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAP1988120.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            55962..56330
                     /locus_tag="ISB30_000442"
     CDS             55962..56330
                     /locus_tag="ISB30_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HAP1988121.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            56368..56673
                     /locus_tag="ISB30_000443"
     CDS             56368..56673
                     /locus_tag="ISB30_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAP1988122.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            56676..57080
                     /locus_tag="ISB30_000444"
     CDS             56676..57080
                     /locus_tag="ISB30_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988123.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            57070..57369
                     /locus_tag="ISB30_000445"
     CDS             57070..57369
                     /locus_tag="ISB30_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988124.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSATRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            57466..57948
                     /locus_tag="ISB30_000446"
     CDS             57466..57948
                     /locus_tag="ISB30_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAP1988125.1"
                     /translation="MILSIDSNDANSAPLHKKTISSLSGAVESMMKKLEDVGVLVARI
                     LMPILFITAGWGKITGYAGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSNFAEGMNSLMFMKNLTISGGFLLLAITGPGAFSIDRLLNKKW
                     "
     gene            58018..59004
                     /locus_tag="ISB30_000447"
     CDS             58018..59004
                     /locus_tag="ISB30_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAP1988126.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGASGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTINNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            59287..59655
                     /locus_tag="ISB30_000448"
     CDS             59287..59655
                     /locus_tag="ISB30_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP1988127.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(59689..60585)
                     /locus_tag="ISB30_000449"
     CDS             complement(59689..60585)
                     /locus_tag="ISB30_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1988128.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            60690..61391
                     /locus_tag="ISB30_000450"
     CDS             60690..61391
                     /locus_tag="ISB30_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAP1988129.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASTGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            61414..61578
                     /locus_tag="ISB30_000451"
     CDS             61414..61578
                     /locus_tag="ISB30_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988130.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(61639..62949)
                     /locus_tag="ISB30_000452"
     CDS             complement(61639..62949)
                     /locus_tag="ISB30_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAP1988131.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(62977..64308)
                     /locus_tag="ISB30_000453"
     CDS             complement(62977..64308)
                     /locus_tag="ISB30_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAP1988132.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLM
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
BASE COUNT        15461 a        17181 c        16499 g        15329 t
ORIGIN      
        1 tgattttatt cgccgactat aacacccctt atcttttcgc tatttccttt gtactcttga
       61 tcggcctttt agaaattttt gcacttatct gcggtcacat gctctccggt gctcttgatg
      121 cacatcttga ccattatgat tccataacca caggtcatat tagccaggca cttcattatc
      181 tcaatattgg aagattgcca gctctcgtcg tcctctgctt actcgctggt ttttttggtc
      241 ttattggcat cctgttgcaa catgcctgcg ttacgctctg gcaatcacca ctccccaacc
      301 tgttcgtggt tcctgtcagt ttactgttta caattattgc agtgcattat accggtaaag
      361 tcgttgcacc ctggattcca cgcgaccata gttcagctat tacagaagaa gaatatgttg
      421 gtagcatggc attaattacc ggccatcaag ccacttcagg taacccctgc gaaggaaaac
      481 tcaccgatca atttggccaa atacattatt tgctactgga gcctgaagaa ggaaaaactt
      541 tcactaaagg agacaaagtg ttaattattt gccgactttc tgcaacgcga tatttggcgg
      601 aaaataatcc ctggccccag atattgtgat ttattaatca aaaggaaatt taataatgga
      661 tgatatcgtt aattctgtgc cctcctggat gtttaccgcg attatcgccg tatgcattct
      721 gtttattatt ggaattattt tcgccaggct ctatcgtcgc gcttcggcag agcaagcatt
      781 tgttcgtact ggtttaagtg ggcaaaaagt ggtgatgagc ggtggcgcaa tcgtgatgcc
      841 gatctttcat gaaataattc ccatcaatat gaataccctg aagctggaag tcagccgctc
      901 aaccattgat agcctgatta cgaaagatcg tatgcgcgtc gatgtcgtgg ttgctttctt
      961 tgtgcgggta aaaccttcag tagaagggat tgccaccgcc gcccagacgc tggggcaacg
     1021 caccctgtcg cctgaagact tacgtatgct ggtggaagat aaatttgtcg atgccctccg
     1081 tgcaacagcg gcacaaatga ccatgcatga gttacaggat acccgcgaga acttcgtgca
     1141 gggagtacaa aataccgtag ctgaagatct gtcgaaaaat ggcctggaac tggaaagcgt
     1201 ttcacttacc aactttaacc agacctcgaa agaacatttc aatccgaaca atgcctttga
     1261 cgccgaaggt ttaaccaaac tgactcagga gacggagcgc cgtcgtcgcg aacgtaacga
     1321 agttgaacag gatgtggaag tcgcagtgcg tgagaaaaac cgtgatgcac tttcgcgcaa
     1381 gttggagatt gaacaacaag aagcgtttat gacgcttgag caggagcagc aggttaaaac
     1441 ccgtaccgct gagcagaatg cgaaaattgc ggcttttgaa gctgaacgtc gtcgtgaagc
     1501 agagcagacg cgaattctgg ctgaacgaca gattcaggaa acggaaatcg accgcgaaca
     1561 ggccgtccgc tcaagaaagg ttgaggccga acgtgaagtt cgcattaaag agatcgaaca
     1621 gcagcaggtc accgaaatcg ctaaccagac gaaatcgatc gctattgccg ccaaatcgga
     1681 acaacagtcc caggcagaag cgcgtgctaa ccttgcactc gcagaagcag taagcgccca
     1741 acaaaacgta gaaaccactc gccagactgc cgaagccgat cgtgctaaac aagtggcgtt
     1801 gatcgccgca gcgcaggatg cagaaaccaa agcggttgaa ctgaccgtgc gggcgaaagc
     1861 agaaaaagaa gccgcagaaa tgcaggcggc ggctatcgtt gagttagccg aagctacacg
     1921 taaaaagggt ctggcggaag cagaagcaca acgtgcgctg aacgatgcta tcaacgtact
     1981 ttctgatgaa caaaccagcc ttaaattcaa actggcatta ttgcaggcgc tgcctgcggt
     2041 aattgagaaa tccgttgagc ctatgaagtc tatcgacggc atcaaaatta ttcaggtcga
     2101 tggtctgaat cgtggcggcg ctacgggtga tgcaaacacg ggtaatgtgg ggggcggaaa
     2161 cctggcagaa caagcgttat cagccgcact ctcttaccgc acacaggcac cgctgattga
     2221 ctccttgctc aatgaaattg gcgtttcagg cggctcactg gcggcattga cttcatcctt
     2281 aagctcaaca actcccgtcg aagaaaaagc agagtaaacc ctcttctatg aataatccct
     2341 ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgatcagg
     2401 ctgttgcagc attttgcgtg actgctcggg gaaggcaaaa ctgcctctgg gaaagcgttg
     2461 cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc
     2521 cctcccgagg aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg
     2581 gttgtgttgt tactgttaag tttcccgact tactaacaac tcatcagagg ggggagaaat
     2641 cctcccttac ccttgttcct ttactttagg ttgaaaaaac aacagcgtca atagacctgc
     2701 ctgctacgaa gcgagatctg tgaaccgctt tccagttagc cttttttatc cagttggatc
     2761 ttcttgatga tgttggtcgt cgagcaaccg tcttcaaagt tgagcaccag cacttcgcca
     2821 ccgttggccc agacttcttt actcccggca atctcttctg gtttatagtc gccgcctttc
     2881 accagcagat ctggcaagat cccggcgatc aagcgctgcg gtgtgtcctc ttcaaacgac
     2941 accacccagt cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta
     3001 acggggcggg aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc
     3061 aaacggtcac ccagtttacg ggcatttgcc agataagaga cgtgcccggc gtgcaggatg
     3121 tcaaagacac cgttggtcat caccactttt tcaccgcgtt tacgcgctgc cgctacagca
     3181 agcttcagtt cctcttcggt catcacacca aagccggtat ctgcacgtcc acgtactgcg
     3241 ttttccagct cgatcggcga aaccgtggat gtccccagtt tgccgaccac cacaccagcc
     3301 gccgcattgg caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc
     3361 aggacgccaa tcaccgtgtc gcccgcaccg gtaacgtcat acacttcctg cgcctgggtt
     3421 ggcatatgca gcggcgcttt acccggttgc agcagcgaca taccctgttc ggaacgggtc
     3481 actaacagag ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct
     3541 tcttcggtct tacatttacc gacaacagct tcaaattccg agagattcgg cgttaacagc
     3601 gtagcgccgc ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacaccc
     3661 gctttacgcg ccagttggat catctgctgt acgcttgcca gcgcaccttt ggcgtagtca
     3721 gaaagcacca gcgcgccaat cgaactcagt gcctgattaa tccgctcgtg cagcggctgc
     3781 ggatcaaccc cttcgaaacc ttcttcgaaa tccagacgga tcagctgttg gttacgggaa
     3841 agtacccgta atttggtgat agtcggatgc gtcggtacag aaacaaagtc gcatttaacg
     3901 ttgacgtcgg ccagagattt actgagcgcg cgcgccgcat cgtcaatgcc cgtcaacccg
     3961 accaggcgtg cattagcacc gagagaagcg atattcatcg ccacgttagc cgcgccgccc
     4021 ggacgttctt cgatggtatt cactttaacc acgggtaccg gcgcttccgg cgagatacgg
     4081 ctggtgggac cgtaccagta acgatccagc atcacatcac caaccaccat cactcctgca
     4141 cgttcaaact ctggcagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc
     4201 gcgataatac catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagtt
     4261 cacgctctgc ggtgaagcaa tcctccgaca catggcccgg caattcctgt aatgccagat
     4321 gatgaagttc atcgcgcagc gtcgtgtaag cacgggtcag cgccatcgct tcctgctctt
     4381 ccataatgtc gttttgcgcc agtagttcca gaatgcgcac gttgtctgac cagcgcgtta
     4441 acttcggttt ttcgtgagcg tagcgcaaca ccagatattg agtaataaat tcgatatcgg
     4501 taattccgcc ttcatcggct ttgatatcaa agcgatcgcg atgtttattg ccgagatggg
     4561 cacgcatttt ctcgcgcatt tcccgcactt ctgtttgcag ggttttgcct tcgcgcggca
     4621 gcgtcataat ctcccggcgt actgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca
     4681 ctacacgcgc acgcaccagc gcctgatgct cccacgtcca ggcctcgttt ttctgataat
     4741 cagcaaatgc ttctgcggat gtcaccagca ttcccgccgc cccggacgga cgcagtcgtg
     4801 catccacttc atacaaaatg ccggaagagg ttcgcgtact gaacagatgc ataatgcgtt
     4861 gcgccagacg cagataaaac tgtcgcccgt cgatttcccg ctcgccgtca gtcatcgcat
     4921 ccattgggca atcatggagg aagatcaggt caagatcgga actgtagcct aactcccagc
     4981 cacccagctt gccgtagccg accaccgcaa aaccacgccc ttcgcgttcg ttcaggtgat
     5041 ttggcttacc gtagcgggca accatttgaa cccacgcctg ctgaacgacg gcatcaatca
     5101 tggcttctgc cagccaggtt aagtgatcgc tcactttcat taccggtagc gtaccggcga
     5161 tatccgctgc cgcaatgcgt aacagctgcg cctgtttgaa ctgacgcagc gcctcaagct
     5221 gttgttcttc gtcatcttcc ggcacgcgca gcaaatactg gcgcagttca tcacggtagg
     5281 catccgtcgc cgttggttgg tagagggtat ttggatcgag caattcatcc agcaataatg
     5341 gataacgcgc tagctggctg gcaatcatcg gcgatgcagc acacagagaa atcaaatgct
     5401 taagcgccgc agggaattca ctaagcagct caagataagt ggtgcgagta acaatcccca
     5461 ccagcaaggc ggtaatgcgc gacagcgtga cagcagcgtc ttcacgcgca cagacatcac
     5521 ttagcagatg tggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atggtgcgct
     5581 tatccagctc tttgcggaaa tcagcaatca gcattagcac ctgtttgcga tcatcctcgc
     5641 taagatgcgc cagcactggc gtggtgtcat cttcctgcaa cgcatcctgc cacagctcac
     5701 gccactgttc cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat
     5761 taaacacccg gcgcacattg gccatatgtg cggtcagcac cccggtcaat tgcggccagt
     5821 cagcaaagtc catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgtct
     5881 gggtttgttc gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatacg
     5941 ccactcgcaa ttgttcagca tcgttttcag aaagcagatg cagcgcggca atggcgctga
     6001 gcgttggcag taaagagcgc gattgcagcg acggttcgcg cccgccgcga atgagctgga
     6061 acacctggac gataaattca atttcgcgaa tgccgcctgc gccaagtttg atgttgtcgg
     6121 tcaaaccgcg tcgacgcact tcacgggcaa tcattccttt catgttgcgc agcgactgaa
     6181 tcacgctgaa atcgatgtaa cgacggaaaa caaacgggcg cagcaacgca cgcaactcgt
     6241 tagcatagac gccgtcgcta tcccccataa tccgcgcctt gaccatcgcg taacgctccc
     6301 agtcgcgccc ctgctcctgg taataatctt ccagcgccgc aaagctcagc accagcgggc
     6361 cactttcccc aaacggacgc agccgcatat ccacgcgata gacgaagcca tcctgcgttg
     6421 gttgatccag tactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca
     6481 gttcccggcg tccgccctgg gtacaaccat gttccggcca ggcaaaaatc agatcgatat
     6541 ctgaggagaa attcaattcc ccgccgccca gcttacccat gcctaaaatc agcagcggtt
     6601 gcgcttcgcc ctgcgcattg cacggcgttc cccattcgcg gcagcaggcg tcatacagcc
     6661 agtcacgcgc cgcaacaatc agcgtctccg ccagatggct gagctgctgc aatatgctct
     6721 cttcagtaac cagtgccagc gtttgcgccc aggcgatgcg caccataatg cgccgccgga
     6781 atagccgcag ctcgcgcatt aacccagcct cgtcactcac attactgagc gcctcctgca
     6841 accatgcagc gtaatgctgc cattcgtcgg cctgcggcgg ttggctttcc agttccgtca
     6901 gccactctgg atgcgcaatc acgctgtcct gcacaaaatc actgaaagta agtactgact
     6961 tcgcctgtgc gctaagtgat tcctcggcta aaggctctgg cagccgctca acaacggtct
     7021 gccagtactg ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat
     7081 taacgttttc cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt
     7141 tcaatttcaa tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc
     7201 cagtcttgcg cgacgacagg atcgtagtaa cccgccagca acagtattga gtcaatatca
     7261 cgcgtcaggc gtggcaattg gtcacggtag cgatcgccta acggctggca gaaaacgctt
     7321 ttcagttcag cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa ggaatcgctc
     7381 attttactct gcgctttggc atctaaaaac ggctgccagg ctttgcttac cagccattcg
     7441 gtcaacgcca gcttcgccat tgccgtttcg gtagagtaga ccgccgttac ggcagaaacc
     7501 gcagaagcaa tggtcgcctc gcattgagtc agcagatcac gtaagtgagt gctcgcttta
     7561 cgcggcacaa tgccaccgaa cagcatcagt gtatgacgaa ccaggccaat ggctgccagc
     7621 acctgctctt tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc
     7681 gctaacgcca gctcgagcgc cgcttccagc ccctgttcca catcagcttt tgccgcaaca
     7741 tgcaaaatgg tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca
     7801 cgcgctgctt tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc
     7861 agtttcagca ccgcacgcat gtcgccgcta agcagttcca gttccagctc acagataggt
     7921 tcagcaaatt cacccgcttt cacctcaccc tgatcgagag cgatttcaat tcggctgcca
     7981 tcgacctcta ctagccattt ttcgcgataa aaatcggtgc taaacagcgg ctgcacgcgg
     8041 gaagcgagat cggtaggcaa ttcgccgttc ggccagactt ccgtcggtaa ctgcgccagg
     8101 tcgagcgtcg gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc
     8161 actcttcctg caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgcaag
     8221 cccatgtcgt gcccacgcag ccaattatcc ggcgtttcgt agtaaatatt cagcaactgc
     8281 acggggtcat ggtgctcgcc gcccagcgta ttgagatggt cacgcagcgc ctcaacggca
     8341 ctgtgattaa caataaactt taattcgatt tcctgagcca tggcctttac ttactggtta
     8401 tgtcacataa gtgacgatga acggcgaact taatgcgatc ttttttgtca gtagatagta
     8461 ttttgcgcca aattgccatg caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg
     8521 caagacctga cacagaggat tccgattgat gtcgaatgct ttgcgtagcg acactgatgc
     8581 cactactatc gttccacttt ccatgacaat aacgacagcc tgatgccaaa attacgcctg
     8641 atcggattaa ctttactcgc acttagcgcg actgccgtct cacacgctga agaaacgcgc
     8701 tatgtttccg acgaactgaa tacctgggtc cgtagcggtc cgggagatca ttatcgcctc
     8761 gtgggcacgg ttaacgccgg cgaggaagtg accttattac aaactgacgc caacaccaat
     8821 tatgcccagg tgaaagacag ctctggccgt actgcctgga tcccgttgaa acaacttagc
     8881 actgaaccaa gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa aactctgacc
     8941 gataaactca ccaatatcga taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa
     9001 gtggcgcaga gtgacagcgt gatcaacagg ttaaaagaag aaaatcagaa actgaaaaac
     9061 gagctgattg tcgcgcagaa aaaggtcgat gccgccagcg tacagttgga tgacaaacag
     9121 cgcaccatca tcatgcagtg gtttatgtat ggcggtggcg tgctggggct tggcttgctg
     9181 cttggtctgg tactgccgca cctgatccca agccgcaaac gcaaagatcg ctggatgaac
     9241 taaatcgcct tcttcgttgc actgtcttat tattaggaat tgtgtatttg acgaagggat
     9301 gatggcgtga agatttatct ggtcggtggt gctgttcggg atgcattgtt agggctaccg
     9361 gtcaaagaca gagattgggt ggtggtcggc agtacgccac aggagatgct cgacgcgggc
     9421 taccagcagg taggccgcga ttttcctgtg tttctgcatc cgcaaacgca tgaagagtat
     9481 gcactggcac gtaccgaacg gaaatctggt tccggttaca ccggttttac ctgctatgcc
     9541 gcaccggatg tctcgctgga agatgatctt aagcgtcgcg atctgaccat taatgcgctg
     9601 gcccaggacg ataacggcga gattatcgac ccgtacaacg gtctgggcga tctgcaaaat
     9661 cgtctgttgc gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt gttgcgcgta
     9721 gcgcgttttg ctgcgcgtta tgcccacctc agtttccgta ttgccgatga aactctggcg
     9781 ctgatgcgcg agatgaccca tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg
     9841 aaagagacgg aaagcgccct caccacccgc aatccccagg tgttcttcca ggtactgcgc
     9901 gattgcggcg cactgcgcgt tttattcccg gaaattgact ccctgtttgg cgttccggcc
     9961 cctgccaggt ggcacccgga aatcgacacg ggtattcata ccttaatgac gctctcaatg
    10021 gcggcgatgc tgagtccgca ggtcgatgtt cgtttcgcaa cgttatgcca cgatctcggt
    10081 aaaggactga cgccgccaga actttggcca cgtcatcatg gtcatggccc ggcgggtgtg
    10141 aagttagtgg aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc
    10201 agactggtag ctgagtttca cgatctcatc cacacctttc caatgctgaa cccgaaaacc
    10261 attgttaaat tgtttgattc cattgacgcc tggcgtaaac cgcagcgtgt cgagcaactg
    10321 gcgctgacca gcgaggctga cgtgcgcggc agaaccggtt ttgaatcagc ggactacccg
    10381 caaggccgct ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac aaaagccgtc
    10441 gttgaagcag gatttaaagg tgtggagatt cgtgaggagc tgacccgacg gcggattgcg
    10501 gcggtagcca gctggaagga acaacgttgc ccaaagcctg attaactttt taaattcagt
    10561 atattgcaga aatcccttag gtcggataag acgcacaagc gtcatatccg acactatctc
    10621 tcaatcaaaa gaacacgacg tacaccgcag ccgccacaat aaagcgataa atggcgaacg
    10681 ggataaacga aatgcgctta atcaattgca ggaaggtttt aatcgctatc agcgccacca
    10741 caaaagcggt gataaaccca acggcaaaca tcgggatatc gccggtagtc aggaagcccc
    10801 agcttttgta gagatcgagc gccgttgcgc ccatcatcat cggcaccgcc agcaggaacg
    10861 agaactcgga agcagcgtaa cggctcaccc ccatcagcat cccacctgaa atggtcgccc
    10921 cggaacggga gaaacccggc cacagcgcca gacactggaa acagccaatc ataaacgcct
    10981 gacgataggt catatcatca agacccggcg cacgcggctc tttcggcttc aggcattcgg
    11041 cagcaatcag caacagaccg ccaacgacca gtgcatacat gacatttatc gggttaaaca
    11101 atgatttaat cgtgtcgtgg aacaacagcc ccagtaccac cgccggaatc atccccagca
    11161 aaatgtggat cagcgttaaa cgacctttgt tttctccttc gtgctgcaac gggcggccaa
    11221 agtggatgcc aatcaggcca aacagacgcc gccagaacat cactactacc gccagaattg
    11281 atcctaactg gatcacgact tcaaaggttt tcgccgtgtc gccctcaaac cccaacaagt
    11341 gaccgacaat aatcatatgg cccgtgctgg ataccggcag aaattctgtc aatccttcga
    11401 ccacacccaa tattgccgct atcagcagcg agtgcatatc gctcatcaat aaacccctaa
    11461 attataaaaa tgtaacgctt gtccgaacta ctgcagtatg accaggttat aatcgtttgg
    11521 tttaacagct gtaaaattaa ttattttctt tcagattatt gccacgctca atgattacgc
    11581 caacattcgc cgcccgcgcc actgcgcctg gcttgctgag tttgatacgc acccacggcg
    11641 agttgaagcg tgctaacagc agttccgcca cctcttcagc cacgcgttcc accagcgcaa
    11701 aacgcgcccc ctcgacgtgg ctgaccaccg tttctgcaat gtcagcgtaa ctgaggcaat
    11761 ccgccacatc atcacttttc gccgctttac ggttatccca cgccatttcg atatcgaaca
    11821 ctaacttctg ttcgatggtc tgttcccagt cgtaaacacc aatagtggtg attaccgaaa
    11881 gttgctctat aaatacaata tccatcacgt cctgcctgct ttttggctaa ccggatacca
    11941 cttccggcga aatgtgcgta ttatccacag attcatcgtt gaacacgaat tttcaaaacg
    12001 gaacagctta tgagtgcaat cgcgcctgga atgatcctca tcgcgtacct ctgcggctcc
    12061 atttccagtg ccattctggt ttgccgcttg tgtgggttac ccgatccgcg aaccagcggc
    12121 tccggcaatc caggcgcaac caatgtgtta cgtatcggtg gcaagggagc agccgtagca
    12181 gtactgattt tcgacgttct gaaaggaatg ttgcccgtct ggggcgcgta tgaattaggt
    12241 gtcagcccct tctggctagg cttaattgcc atcgccgcct gtcttggaca catctggccc
    12301 gttttcttcg gttttaaagg aggaaaaggc gttgctaccg cttttggtgc catcgcacca
    12361 attggctggg atctcactgg agtaatggcg ggaacctggt tactgaccgt gctattgagt
    12421 ggttactcgt cgctgggagc gattgtcagt gcactgattg ctccgtttta tgtctggtgg
    12481 tttaagccac aattcacctt cccggtttcg atgctctctt gcctgatcct gctgcgtcat
    12541 catgacaaca tccagcgtct gtggcgtcgc caggagacaa aaatctggac gaaattcaaa
    12601 agaaagcgcg aaaaggatcc cgagtgattt ctggtggatc tacatgacct gatagccttc
    12661 gtcgggcttg cccagccgtt gctggcacca tgccgccaga aattccacac agacacgtaa
    12721 tttcatgctg cgatagagcg gctcccggta aacagcccag atattggcgc tttgtgcata
    12781 ctccggcaat actcgcacca gtttgccact ctccagaaac ggcaacacat cccactcgga
    12841 acgcagcata atccctttgc cctccagcgc ccattgcaac acaatctcgc cgctattgga
    12901 ggaaagatgt ccgcttacct tcaccgattt tttctcctga ccgttcccca actcccatat
    12961 tccgtgggtc atatcgcgct ctttggtcac caggcagtca tgacgactta attcctgtaa
    13021 ggattgtggc tgcggatatt tttgcagata ctcaggtgct gcgcataata ttcttttatt
    13081 ttttgtcaaa agatgcgcaa tataataatc aggaattgcg tcattaatac gaatatccag
    13141 atcaatatta tcctgcacta aatcaatttg tcgatcgaac aattcaaaat gcacctgtaa
    13201 ctcaggataa ttgcgcatca gttcggtaat agctggcgca atatggctgc gcccaaaacc
    13261 aaaactgcaa ccaatgcgga tcatcccctc cgggcgcgtt ttgatttgcg tgacgtcatc
    13321 gaccagtcgc tgatattggg tgaggatttc cagcgcatgt tcgtaacaac gttggccact
    13381 ttcagtcagc gccacgccgc gagcggagcg gttcagcagc gttgttgcca gcgtattttc
    13441 aaggatttgg atccgcttgg tgacaaatgc aggcgtttgc ccaagcgtcg ccgctgcagc
    13501 actaaaactc ccgctatgga cgatttccac cagtacctgc aagtctttgg ctaaaggcca
    13561 gctatttagc attgttaaat atccgcagtt ttgataaacg cagtgtattt cgcaaaacat
    13621 taccattccc tgctctggcg caatattcgt tcttattttc gcgttgattc acgaactgtt
    13681 aattacatat tcacggtggc aaaaaatata aaaccacatt tttagtggta gtttgtggcg
    13741 gtgaattttt ccagacaaat acataaactg gagttgccat gatgagcgaa agtaataagc
    13801 aacaggcagt gaataagttg acggagattg tcgctaactt taccgccatg atttctaccc
    13861 gaatgcctga tgacgtggtg gataaactaa aacagctaaa ggatgccgaa acatcgtcga
    13921 tgggaaaaat catctaccac acgatgttcg ataacatgca aaaagcgatc gacctgaatc
    13981 gtcctgcctg tcaggacacc ggcgaaatca tgttttttgt taaggtcggt tcccgtttcc
    14041 cactgcttgg cgagctgcaa agcatactca aacaagccgt ggaagaggca accgtcaaag
    14101 cgccactgcg tcacaatgcg gtagaaattt ttgacgaagt aaacaccggc aaaaataccg
    14161 gcagcggcgt accgtgggtc acctgggata tcgtccccga cggtgacgat gcggaaatcg
    14221 aagtttacat ggcaggcggc ggctgcacgc tacctggccg atcgaaagtg ttaatgccgt
    14281 cagaaggcta cgaaggcgta gtgaaattcg tcttcgaaaa tatctccacc ctcgccgtaa
    14341 acgcctgtcc gccggtactg gtgggcgttg gcatcgctac ctcggtggaa accgccgcag
    14401 tgctctcgcg aaaagccatt ttgcgcccga ttggcagccg ccaccccaat ccgaaagcgg
    14461 cagagctgga gctacgcctg gaagaaggac tcaaccgttt ggggattggt ccacaagggc
    14521 tgactggcaa cagttcagtg atgggcgtgc atatcgaatc tgccgctcgc catccgtcaa
    14581 ccatcggcgt tgctgtttct accggctgct gggcgcatcg tcgcggcaca ctgctggttc
    14641 atgccgatct cacctttgaa aatctgtctc acacccggag cgcgttatga aaaagatcct
    14701 gacaaccccg atcaaagctg aagatctgca agatattcgc gtcggcgatg tgatctacct
    14761 gaccggtacg ctggtgacct gccgtgacgt ttgtcaccgt cgcttgatcg aactgaaacg
    14821 tccgatccct tacgatctca acggcaaagc gattttccac gctggcccca tcgtgcgcaa
    14881 aaacggcgac aaatgggaga tggtatccgt cggcccgaca accagtatgc gtatggaaag
    14941 ttttgaacgt gaatttatcg agcagaccgg cgtgaaactg gtggtcggca aaggcggaat
    15001 ggggccgctg accgaagaag gctgccagaa attcaaggcg ctgcatgtga ttttcccggc
    15061 tggctgtgcg gtactggcgg caactcaagt ggaagagatt gaagaagtgc actggacaga
    15121 actcgggatg ccagagtcgc tgtgggtctg ccgggtcaaa gagtttggcc cgctgattgt
    15181 ctctatagat acccacggca acaacctgat agccgaaaat aaaaagctgt tcgccgaacg
    15241 ccgcgatccc atcgtggaag agatctgcga gcacgtccac tacatcaaat aaccctcacg
    15301 gagaggctcg cccctctcct ttttcgcagg cataacacga tgaaaccttc cactgaatgg
    15361 tggcgatacc ttgcgccgct ggcggtcatc gccattattg ctctacttcc gcttcccgca
    15421 gggctggaga gtcatacctg gctctacttt gccgttttta ctggcgtgat cgttggactg
    15481 atcctcgaac ccgtgccggg tgccgtggtg gcgatggtgg gtatatccat catcgccatt
    15541 ctttcgccgt ggctgctgtt tagcccggag cagctcgccc agccaggttt taagttcacc
    15601 gccaaatccc tctcctgggc ggtttctggt ttttctaatt cagttatctg gcttattttc
    15661 gccgccttta tgtttggtac gggctatgaa aaaaccgggc ttggacgtcg catcgcgctg
    15721 attctggtga aaaaaatggg gcatcgcacg ctgtttctcg gctatgcagt gatgttctcc
    15781 gagctgatac tcgcgcctgt aacaccgtcc aactcggcgc gtggtgcggg gattatctat
    15841 cccatcatcc gtaacctgcc accgctctat caatcacaac caaacgacag cagttcgcgc
    15901 agcattggct cgtacatcat gtggatgggg attgttgccg actgcgtgac cagcgccatt
    15961 ttcctgacgg cgatggctcc aaatttgctg ttaattggac tgatgaaaag cgcatctcac
    16021 gccatgctaa gctggggcga ctggttcctc gggatgttgc cgctcagcat tttactggtt
    16081 ttgctggttc cctggctggc ttacgtgctg tacccgccgg tactgaagtc tggcgatcag
    16141 gtgccgcgct gggcagagac ggaactgcag gcaatgggcc cgctctgttc gcgtgaaaaa
    16201 cggatgctgg ggctgatggt aggcgcactg gtgttgtgga tttttggcgg tgattatatc
    16261 gatgccgcga tggtcggtta cagcgtggtg gcgctgatgc tgcttctgcg cattatcagt
    16321 tgggacgaca ttgtcagtaa taaagcggcg tggaacgttt tcttctggct ggcctcgctt
    16381 atcaccctcg ctaccggact caacaacacc ggttttatta gctggtttgg caaactgtta
    16441 gcaggcagct taagcggtta ttcgccaacg atagtgatgg tggcgttgat tgtggtcttt
    16501 tatctgctgc gctacttttt tgccagcgcc acagcgtata cctccgctct cgcaccgatg
    16561 atgattgccg ccgcgctggc gatgccggaa atcccgctgc cggtgttctg cctgatggtt
    16621 ggtgcggcaa ttggtctggg gagcattctt acaccatacg ccaccggacc cagtccgatt
    16681 tactacggca gtggttatct gccaacggcg gattactggc gactgggggc gatttttggg
    16741 ctgatattcc tcgtattgct ggtgattacc ggcttactgt ggatgcccgt ggtgttgctt
    16801 taagtgagga cggtgccgga aattgctctc cggcacccaa tagttttacg cggccggtaa
    16861 ctctgccagt ggccagcgcg gacgcacact cacgccgaga tccgccgtcg cgcctgcttt
    16921 aaaccgcacc attccggcat aggcgatcat cgcaccgtta tccgtacaaa attccggacg
    16981 cgcgtagaac acttcgccac ggcgtttttt catcatttcc gccagtttcg cccgcaacgt
    17041 gcggttagca ctcacgccgc ctgccatgac cagtcgctta aagcccgtct gatccagtgc
    17101 tcgcttacac ttaatcatca acgtatcgac cactgcatct tcaaaggcgc gggcgatatc
    17161 ggcacgcgtc tgatcgtcag tgccgttgtc acgtatggta tttgccgcga aggttttcag
    17221 accagaaaag ctgaaatcca gccccggacg gtcggtcatc ggacgcggga aaacaaagcg
    17281 cccggcagta ccctgagccg ccattttaga cagtaacggt ccgccaggat aatccagccc
    17341 cagcagcttc gcggttttat caaacgcttc accagcagca tcatcgatag actcgccgag
    17401 cagctcgtac tgaccaatgc cagtcacgct gattaactgc gtatgaccgc cggaaaccag
    17461 cagcgcgaca aacggaaatt ccggtgggtt atcttccagc atcggcgcta acagatgtcc
    17521 ttccatatga tgtaccggga ttgccggaac gtcccaggca aacgccagag aacgccccac
    17581 ggtcgcgcca accagcagtg cgccgactaa tcctgggcct gcggtatagg ccacagcatc
    17641 aatgtctttt gccgttaaac cagactcctt tagcgcctcc tggatcaacg gtacggtttt
    17701 acgcacgtga tcgcgggagg ccagttcagg cacgacgccg ccgtagtcag cgtgcaattt
    17761 cacctgacta tacaattggt tggctaacaa acctttttca tcgtcgtaaa tggcgatgcc
    17821 ggtttcatcg caggaagttt caatacccag tacacgcatg acttgtttta cctcgcttta
    17881 ttaccgcgca gtgtaggacc aatgcgggtt gatgtaaaac tttgttcgcc cctggagaaa
    17941 gcctcgtgta tactcctcac ccttataaaa gtccctttca aaaaaggccg cggtgcttta
    18001 caaagcagca gcaattgcag taaaattccg caccattttg aaataagctg gcgttgatgc
    18061 cagcggcaaa ccgaattaat caaaggtgag aggcacatgc cggtaattaa agtacgtgaa
    18121 aacgagccgt tcgacgtagc tctgcgtcgc ttcaagcgtt cctgcgaaaa agcaggtgtt
    18181 ctggcggaag ttcgtcgtcg tgagttctat gaaaaaccga ctaccgaacg taagcgcgct
    18241 aaagcttctg cagtgaaacg tcacgcgaag aaactggctc gcgaaaacgc acgccgcact
    18301 cgtctgtact aattccccga gagcgttgct ctccgatcag accgagttgt agttgtaagg
    18361 ccgtgcttcc gaaaggaatg cgcggcttat tttcgtttat gaattgctaa aaatcggggc
    18421 ctatggctgg acgaatccca cgcgtattca ttaatgatct gctggcacgc actgacatcg
    18481 tcgatctgat cgatgcccgt gtgaagctga aaaagcaggg caagaatttc cacgcgtgtt
    18541 gtccattcca caacgagaaa accccgtcat tcaccgttaa cggtgagaaa cagttttacc
    18601 actgctttgg atgtggcgcg cacggcaacg cgatcgactt cctgatgaac tacgacaagc
    18661 tcgagttcgt cgaaacggtc gaagagctgg cagcaatgca caatcttgaa gtgccatttg
    18721 aagcaggcag cggccccagc cagatcgagc gccatcagag gcaaacgctt tatcagttga
    18781 tggacggtct gaatacgttt taccaacaat ctttacaaca acctgttgcc atgtctgcgc
    18841 gccagtatct ggaaaaacgc ggattaagcc acgaggttat cgctcgcttt gcgattggtt
    18901 ttgcgccccc cggctgggac aacgtcctga agcggtttgg cggcaatcca gaaaatcgcc
    18961 agtcattgat tgatgcgggc atgttggtca ctaacgatca ggggcgcagt tacgatcgtt
    19021 tccgcgagcg ggtgatgttc cccattcgtg ataaacgcgg tcgggtgatt ggttttggcg
    19081 gacgcgtgct gggcaacgat acccccaaat acctgaactc gccggaaacg gacattttcc
    19141 ataaaggccg ccagctttac ggtctttatg aagcgcagca ggataacgct gaacccaatc
    19201 gtctgcttgt ggtcgaaggc tatatggacg tggtggcgct ggcgcaatac ggcattaatt
    19261 acgccgttgc gtcgttaggt acgtcaacca ccgccgatca catacaactg ttgttccgcg
    19321 cgaccaacaa tgtcatttgc tgttatgacg gcgaccgtgc aggccgcgat gccgcctggc
    19381 gagcgctgga aacggcgttg ccttacatga cagacggtcg tcagctacgc tttatgtttt
    19441 tgccggatgg cgaagaccct gacacgctgg tacgaaaaga aggtaaagaa gcgtttgaag
    19501 cgcggatgga gcaggcgatg ccactctccg catttctgtt taacagtctg atgccgcaag
    19561 ttgatctgag tacccctgac gggcgcgcac gtttgagtac gctggcactg ccactgatat
    19621 cgcaagtgcc gggcgaaacg ctgcgaatat atcttcgtca ggaattaggc aacaaattag
    19681 gcatacttga tgacagccag cttgaacgat taatgccaaa agcggcagag agcggcgttt
    19741 ctcgccctgt tccgcagcta aaacgcacga ccatgcgtat acttataggg ttgctggtgc
    19801 aaaatccaga attagcgacg ttggtcccgc cgcttgagaa tctggatgaa aataagctcc
    19861 ctggacttgg cttattcaga gaactggtca acacttgtct ctcccagcca ggtctgacca
    19921 ccgggcaact tttagagcac tatcgtggta caaataatgc tgccaccctt gaaaaactgt
    19981 cgatgtggga cgatatagca gataagaata ttgctgagca aaccttcacc gactcactca
    20041 accatatgtt tgattcgctg cttgaactgc gccaggaaga gttaatcgct cgtgagcgca
    20101 cgcatggttt aagcaacgaa gaacgcctgg agctctggac attaaaccag gagctggcga
    20161 aaaagtgatt taacggctta agtgccgaag agcgatcggg aagcccccga cagccgcact
    20221 gagaggcagc ggcaaatata taagtacgcc ctcgtaatta tcgttggcgg taaacaaccg
    20281 ttggatttca gcgttaacgg ctgaaggaca tcgggtcaat cgcccaacac caacctcatg
    20341 aaataagtgt ggataccgtc ttatggagca aaacccgcag tcacagctga aacttcttgt
    20401 cacccgtggt aaggagcaag gctatctgac ctatgccgag gtcaatgacc atctgccgga
    20461 agatatcgtc gattcagatc agatcgaaga catcatccaa atgatcaacg acatgggcat
    20521 tcaggtgatg gaagaagcac cggatgccga tgatctgatg ctggctgaaa acaccgcgga
    20581 cgaagatgct gccgaagccg ccgcgcaggt gctttccagc gtggaatctg aaatcgggcg
    20641 cacgactgac ccggtacgca tgtacatgcg tgaaatgggc accgttgaac tgttgacccg
    20701 cgaaggcgaa attgacatcg ctaagcgtat tgaagacggg atcaaccagg ttcaatgctc
    20761 cgttgctgaa tatccggaag cgatcaccta tctgctggaa cagtacgatc gtgttgaagc
    20821 agaagaagcg cgtctgtccg atctgatcac cggctttgtt gacccgaacg cagaagaaga
    20881 tctggcacct accgccactc acgtcggttc tgagctttcc caggaagatc tggacgatga
    20941 cgaagatgaa gacgaagaag atggtgacga cgataccgcc gatgatgaca acagcatcga
    21001 cccggaactg gctcgcgaaa aatttgcgga actgcgcgct cagtacattg taacgcgtga
    21061 caccatcaaa gcgaaaggcc gcagtcacgc tgccgctcag gaagagatcc tgaaactgtc
    21121 tgaagtattc aaacagttcc gcctggtgcc gaagcagttt gactacctgg tcaacagcat
    21181 gcgcgtcatg atggaccgcg ttcgtacgca agaacgtctg atcatgaagc tctgcgttga
    21241 gcagtgcaaa atgccgaaga aaaacttcat taccctgttt accggcaacg aaaccagcga
    21301 tacctggttc aacgcggcaa ttgcgatgaa caagccgtgg tcggaaaaac tgcatgatgt
    21361 ctctgaagaa gtgcatcgcg ccctgcagaa actgcagcag attgaagaag aaaccggcct
    21421 gaccatcgag caggttaaag atatcaaccg tcgtatgtcc atcggtgaag cgaaagcccg
    21481 ccgtgcgaag aaagagatgg ttgaagcgaa cttacgtctg gttatttcta tcgccaagaa
    21541 atacaccaac cgtggcttgc agttccttga cctgattcag gaaggtaaca tcggcctgat
    21601 gaaagcggtt gataaattcg aataccgccg tggttacaag ttctccacct acgctacctg
    21661 gtggatccgt caggcgatca cccgctctat cgcggatcag gcgcgcacca tccgtattcc
    21721 ggtgcatatg attgagacta tcaacaagct caaccgtatt tctcgccaga tgctgcaaga
    21781 gatgggccgt gaaccaacgc cggaagaact ggctgaacgc atgctgatgc cggaagacaa
    21841 gatccgcaaa gtgctgaaga tcgccaaaga gccaatctcc atggaaacgc cgatcggtga
    21901 tgatgaagat tcgcatctgg gggatttcat cgaggatacc accctcgagc tgccgctgga
    21961 ttctgcgacc accgaaagcc tgcgtgcggc aacgcacgac gtgctggctg gcctgaccgc
    22021 gcgtgaagcg aaagttctgc gtatgcgttt cggtatcgat atgaacaccg accacacgct
    22081 ggaagaagtg ggtaaacagt tcgacgttac ccgcgaacgt atccgtcaga tcgaagcgaa
    22141 ggcgctgcgc aaactgcgtc acccgagccg ttctgaagtg ctgcgtagct tcctggacga
    22201 ttaatcggta ggccggatca ggtgttacgc cgcacccggc actagtccct ccgcacaaac
    22261 gccacctttt cggtggcgtt tttatcgccc acgcaccacc agcgcctggt ccagctcgcg
    22321 atacgcttca accagtttct ccagtgaaac gcgacttaaa ccgctgggat ttggcagcac
    22381 ccaaatctgc gtcgaaccaa tggtgagcgt ttgtttcccc cactgtgcac cgcgctggct
    22441 gaatccctgt tcatatgctt gtttgcccag aatcgccaac gcctgcggct gataatcttc
    22501 aattttttca atcagcttac gcccgcctgc gtgtagctcc tgctttgaaa cttcattggc
    22561 ttgcaccgtt ggacggtcta ccagtttggt gacgccacaa cgataatcca gcagatgctg
    22621 tgcctcctgc ggcttcaact gacggtcggt aaacccggcc tgatatatca ccttccagaa
    22681 gcgatttgcc ggatgagcaa agggaaaacc agtcccggcg gatgaaagac cagggttgat
    22741 accgcaaaac acgacccgta accctggagc caaaatatcc tcaaccatat ttactccttt
    22801 ctaacatcac caggaaaagt ataacggatt gaaaatacat tgtttataaa aacagcaggc
    22861 gcgcggtaat ggctggattg cgacacggag ttactttata atccgctacc atggcccctt
    22921 agctcagtgg ttagagcagg cgactcataa tcgcttggtc gctggttcaa gtccagcagg
    22981 ggccaccaga catagcaaag gctgacgaga aatcgtcagc ctttttcttt ttatatatcc
    23041 gttactttgc gtgccagtaa gccgccgcac gtacccgctg tgggtcatac tgttccgttt
    23101 caaagcggcg gcttaaattc ttaacgactt tgccttcgcc ggttatccag atgaagtaat
    23161 catcggcagg gatttgcatc tgcgccagac gcgcatctac cgcctgctca tcatgtgcca
    23221 gccattcgat attaaaacca tcaaggtgcg cgagataatc ctgacaggcg ttatcccgca
    23281 cgctaaccag cgcactaact tgcggtttaa cagcaagttt gctcaacgtt tccaggcggc
    23341 ggcgcagtgc gggcattccg gattcatcgc agacatacag ctgatacgcg taatcttccg
    23401 gcaccaccag cgaaccgcgc ggacctgcca ccgtaagttt atcgcccggt ttcgcctgca
    23461 tcgcccagcc gctggcgacc ccaccgtcgt gaataaaaaa atcaatcgcc agttcatggc
    23521 gtagttcgtc atacagcggc gtatagtcac gcgacggtgg gcgtgatcct tccggccaga
    23581 cgatgccctc ttccgttacc gttggcggca caaagtgagc gtcaggttga ggaaagaaga
    23641 gtttgctgtg atcgtcaaag ccgcgcgatg taaaaccgtc cagcgcctcg ccgccgagga
    23701 caatgcgctg aaaaccggcg ctgatgcgct caacgcgtag cacagtcagt tcacggaagc
    23761 gcagatcatt gcgaacgcgc tgtgggtagc ggggggtgtt attcattgtt atcgccttcg
    23821 tgatggtaat cagatatatc taaataaatt ccacaaatga taatgattgt taatcatgat
    23881 aaatgcaagt gatttgtagc actgatatat ctgtagtctg ataagacgcg caacctctta
    23941 tcaagcatca ttgctcaatg ccggatgcgg cgtgaacgcc ttatccggcc tacatgttga
    24001 gtaaacctgc gtttaaaaat catttttgta cttgcagtaa tgctcatatc agatataaat
    24061 tagatatatc taattaagca aaaggaggct gatatgagcc atcatcacga agggtgttgt
    24121 aaacatgaag gccagccacg ccatgagggc tgctgcaaag gtgagaagtc agaacacgag
    24181 cactgcggac acggtcacca gcatgaacac ggtcaatgct gcggtggtcg ccacggtcgc
    24241 ggcggcggtc gtcggcaacg tttctttggt cacggtgaat tgcgtctggt gattctggat
    24301 attctctcgc gcgatgacag ccacggttac gagttgatta aagcgattga aaatctgacc
    24361 caggggaatt acaccccaag cccgggcgtc atctacccga cgctggattt tctgcaggag
    24421 cagtcgctga ttaccatccg cgaagaggaa ggtggtaaga agcagattgc gctgaccgaa
    24481 caaggcgcgc agtggctgga agaaaaccgc gaacaggtag agatgattga agaacgcatc
    24541 aaagcgcgtt gcgttggcgc tgcgctgcgc cagaacccgc aaatgaagcg ggcgctggat
    24601 aattttaaag cggtgctgga tttatgcgtc aaccagagcg atatcactga tgcacaaata
    24661 aaaaagatca ttgcggtgat cgaccgcgcc gcttttgata ttacccaact ggattaatca
    24721 ccgattgccg gatgcggcgt accctacaca ccccgtaggc ctgataagat gcgccagcat
    24781 cgcatcaggc attgtgctcc aaccgccgga tccggcatac cgattaatgc agcaccgtca
    24841 ccgcgtcttc cagtcggctg gcgcggtgtt tcaccatcgc cgacgcctgc gcactctctt
    24901 ccactaactc ggcgtttttc tgggtgatca ggttaagctc atccactgca cgggtcaggc
    24961 tggaaagccc atcggcctgt tccagcgttg aatggctaat ctgagctatc aactgggtga
    25021 cgttttttac ctgcgccaca atatcttcca tcgtccgtcc ggcggcgtgt acctgctgcg
    25081 aaccagattg caccttatca gcactggcat caatcagctt gcgaatatcg ttggcagcat
    25141 tggcgctgcg gctggctaaa tgacgcactt cccccgccac cactgcaaaa cctttgccct
    25201 gttcgccggc acgcgccgct tccaccgccg cattcagggc cagaatattg gtctgaaacg
    25261 caatatcgtt aatcagcgaa gtaatggtgc caatgcgctg ggtactgtcg gcaatatcgt
    25321 ccattgtctt gatcaccgta gtcatcgcct ccccgccctg cactgccgca ttactggcgg
    25381 tgatagaaag tttatcggca gccgacgccg tggcagagtt ctgtttcacc gacgccgcca
    25441 tttggttcat ggtcgccacc gtttgctgaa cgttatcaac tgtctgctgg gtatgttcgt
    25501 tcagttcatc ggtgcctttc gccagcgtct cactgccgtt tctgacactg gacacctggc
    25561 ttgagacatc attaattagc caacggcaca tcaggccaag ctgccctacc gcgcgtaatg
    25621 tcagccccag ctcatcgctg cgcttcagat gctcaacact attacgttct ccggtcgcca
    25681 ctttcagtgc ctggctggcg acattttcta tcggacgtac aatttgccat tcaaaacagg
    25741 catttcccaa caacatcact aatgcacaga agatatacgt caccaccggg gcagcaacaa
    25801 accagagcat ggccgccagc acgataaaca tcagggtcat cactccacgc gcccgccagc
    25861 gaagcggtaa tgaaggcagt ttacccagcc agcctttacg caccaccagg cctttatgaa
    25921 tacgcttacc ggtacgtccg gcattcagcg ctttgtacag cggctccacc gccgcgatct
    25981 cttcatccgt cgcccgggta cgaatcgaca tatagccact gatttttccc tcgcgcacca
    26041 tcggtaccgc attggcccgc acccaataat ggtcgccatt tttgcggcga tttttcacga
    26101 tgccgctcca gggttcccct tttttcaggg tgaaccacat atccgcaaac gccgcttttg
    26161 gcatatccgg gtgacgcacc atgttgtgcg gttgcccctg taactcctgc aaggtatagc
    26221 cgctcacctg cacaaaagtg tcattagcat gagtgatata gctttgcaga tcggtagtgg
    26281 acatcagagt ggtgtcgtcc gccagcgggg tattttgctg ggtgacatac ggatgagaag
    26341 acatgttcgc gtcctgtgca ggttatatgg ttgttaactt cttgtcagag tttatgtcgg
    26401 ccccgctgcg gttatcttta accaattaat ttgatttaga tcgcaatttg cgattaaagc
    26461 acaaatctaa ttccttgatt taaaatactt tcactctgtt actatacgaa aacgttaatt
    26521 atcttgccca aaaatcaggc aattattgcc ctgaaagcgt gcatttgcgc agcaatcatc
    26581 aaatccatac ccgacaaaaa ccgtgcaaaa taacagcaaa tgttagcaaa tagcattaaa
    26641 tattgcgcaa atgataacca catttgtgtt tatcccgatt ttcgcgatcg cagccggagt
    26701 ggcgcaatcc ctgcaatact taaatcggta tcatgtgata cgcgagcctc cggagcatat
    26761 tttgaacagg ttaccttcga gcgcatcagc tttagcgtgc agcgcccacg ccctgaatct
    26821 cattgaaaag cgaacgttgg atcatgagga gatgaaagca cttaaccggg aggtgattga
    26881 atacttcaaa gagcatgtca atccggggtt tttagagtat cgcaaatctg ttaccgccgg
    26941 cggggattac ggagccgtag agtggcaagc gggaggttta aatacgcttg tcgacaccca
    27001 gggacaggag tttatcgact gcctgggagg ttttggaatt ttcaacgtgg ggcaccgtaa
    27061 tccagttgtg gtttccgccg tacagaatca acttgcgaaa caaccgcttc acagccagga
    27121 gctgcttgat ccgttacggg cgatgctggc gaaaaccctt gcggcgctaa cgcccggtaa
    27181 actgaaatac agcttcttct gtaatagcgg caccgaatcc gttgaagcgg cgctgaagct
    27241 ggcgaaggct tatcagtcac cgcgcggcaa gtttactttt attgccacca gcggcgcgtt
    27301 ccacggtaaa tcacttggcg cgctgtcggc cacggcgaaa tcgaccttcc gcaaaccgtt
    27361 tatgccactg ctgccagggt ttcgtcatgt accgtttggc aatatcgaag ccatgcgcac
    27421 ggctcttaac gagtgcaaaa aaaccggtga tgatgttgct gcggtgatcc tcgaaccgat
    27481 tcagggcgaa ggtggcgtaa ttctgccgcc gccaggctat ctcaccgccg tgcgtaagct
    27541 gtgcgatgag ttcggcgcac tgatgatcct cgatgaagta caaacgggca tggggcgcac
    27601 gggcaagatg ttcgcctgcg agcatgagaa cgtacagccg gatatccttt gtctggcaaa
    27661 agcgctcggc ggcggcgtga tgccgattgg cgcaaccatc gccactgaag aggtgttctc
    27721 agtcctgttc gacaatccgt tcctgcacac taccaccttt ggcggcaacc cgctggcctg
    27781 tgcggcggcg ctggcgacca tcaatgtgtt gctggagcag aacttaccgg ctcaggctga
    27841 acaaaaaggc gatatgttgc tggacggttt ccgtcaactg gcgcgggaat atcccgatct
    27901 ggtacaggaa gcgcgtggta aagggatgtt gatggcgatt gagtttgttg ataacgaaat
    27961 cggctataac tttgccagcg agatgttccg ccagcgcgta ctggtggccg gaacactcaa
    28021 caacgctaaa acgatccgca ttgaaccgcc actgacactg accattgaac agtgtgaact
    28081 ggtgatcaaa gcggcgcgta aggcgctggc ggccatgcga gtcagtgtcg aagaagcgta
    28141 atatcaaatc ggatggcgat acgacgtcgc catccgatct ttttatacga tacgtacgcc
    28201 cgcaggcatc atccgctccg gcgttaacag cacgctttca ctgccgtcat cggtttccgc
    28261 acacaacagc atgcattccg acgtttcacc gcgcattttc gctttttgca gattgcataa
    28321 gaccaccact gtttttccca tcagctcttc ttcgctgtag tacggcacca gactggtcac
    28381 ggtttgcagc gttttttccc ccacatcaac ctgtacgatg tacagcttgt cggcgttttc
    28441 atggcgtttc acttccacaa tctttccgac gcgcatttcc agacgtgcaa aatcagcgta
    28501 agccacggtt tccattgttt cctcccttca agtaaaattt tactaaacta tagcaaaagt
    28561 ttttctcaat cctgtaggct aaaaatggag aatgcaggcg tgatcacatt cgtgagccgc
    28621 tgtgttaccg tcacagcgtc aaagaaacgc gctttattta ctgaaaacag gtgacccgat
    28681 aagcacttcc tctacaatgg gggcgcacat cagggaaagt aaaaaaggta aacatggcaa
    28741 cactaaaaga catcgcaatc gaagctggcg tatccctggc gacagtatcc agggtcttga
    28801 atgacgatcc gacattgaat gtgaaagaag agacgaaaca tcgcattctc gagatcgccg
    28861 aaaagctgga gtacaagacc agtagcgccc gtaaactcca gacaggcgca gtcaaccaac
    28921 accatattct ggctatctac agctaccaac aggagttgga gatcaacgat ccttactatc
    28981 tggcgatccg ccacggcatt gaaactcagt gcgaaaagct ggcgatcgaa ctgaccaact
    29041 gttatgaaca caacggctta ccagacatta aaaacgtcac cggtatttta attgtcggca
    29101 aacccacgtc cgccctgcgc gccgccgcct gtgcgttgac cgacaatatc tgttttatcg
    29161 actttcacga acccggcagc ggttacgatg cggtggatat cgatctggca cgtatcagta
    29221 aagaaatcat cgatttctat atcaatcagg gcgttaatcg tattggtttt attggcggtg
    29281 aagacttacc cggcaaggcg gatattcgtg aggtcgcctt tgcggaatat ggtcgtctga
    29341 aacacgtggt acgcgaagag gatatctggc gcggcggttt ttccagttcg tcgggctatg
    29401 aactggcaaa acagatgctg gcgcgggaag actatccgaa ggcactgttt gttgcttccg
    29461 attccattgc tatcggcgta ctgcgggcaa ttcatgaacg tggcctgaac atcccacagg
    29521 atatttcgct tatcagcgtt aacgatatcc ccaccgcgcg atttaccttt ccgccgctct
    29581 ccaccgtgcg catccattcc gagatgatgg gaagtcaggg cgttaacctg gtgtatgaaa
    29641 aagcccgcga tggtcgcgcg ctgccgctgt tggtcttcgt tcccagcaaa ttaaaactgc
    29701 gcggcacgac ccgttaaatc cccttacaca ctgtccggca atcgtttttg ccggacagtg
    29761 ctgccgttta ttttcgtgat ccagttaaag taaatgcatt tacctgctat tttttagtaa
    29821 aaattttact aaactcccca gcaattacac aaactaccat caccataaat ggttccgatt
    29881 tctctctacc gggaggccct atgaatcgct gggaaaacat tcagctcacc cacgaaaacc
    29941 gacttgcgcc gcgtgcgtac tttttttcat atgattctgt tgcgcaagcg cgtacctttg
    30001 cccgcgaaac cagcagcctg tttctgtcct taagcggtca gtggaatttc cacttttttg
    30061 accatccgct gcaagtacca gaagccttca cctctgagtt aatggctgac tgggggcata
    30121 ttaccgtccc cgccatgtgg caaatggaag gtcacggcaa actgcaatat accgacgaag
    30181 gttttccgtt ccccatcgat gtgccgtttg tccccagcga taacccaacc ggtgcctatc
    30241 aacgtatttt caccctcagc gacggctggc agggtaaaca gacgctgatt aaatttgacg
    30301 gcgtcgaaac ctattttgaa gtctacgtta acggtcagta tgtggggttc agcaaaggca
    30361 gtcgcctgac cgcagagttt gacatcagcg cgatggttaa aaccggcgac aacctgttgt
    30421 gtgtgcgcgt gatgcaatgg gcggactcta cctacgtgga agaccaggat atgtggtggt
    30481 cggcggggat cttccgcgat gtttatctga tcggaaaaca actcacgcac attaacgatt
    30541 tcaccgtgcg caccgacttt gacgaagcct attgcgatgc cacactttcc tgcgaagtgg
    30601 tgctggaaaa tctcgccgcc tcccctgtcg tcacgacgct ggaatatacc ctgtttgatg
    30661 gcgaacgcgt ggtgcacagc agcgccattg atcatttggc aattgaaaaa ctgaccagcg
    30721 cccgctttga ttttactgtc gaacagccgc agcaatggtc agcagaatcc ccttatcttt
    30781 accatctggt catgacgctg aaagacgccg acggcaacgt tctggaagtg gtgccacaac
    30841 gcgttggctt ccgtgatatc aaagtgcgcg acggtctgtt ctggatcaat aaccgttatg
    30901 tgatgctgca cggcgtcaac cgtcacgaca acgatcatcg caaaggccgc gccgttggaa
    30961 tggatcgcgt cgagaaagat ctccagttga tgaagcagca caacatcaac tccgtgcgta
    31021 ccgctcacta cccgaacgat ccgcgttttt acgaactgtg tgatatctac ggcctgtttg
    31081 tgatggcgga aaccgacgtc gaatcgcacg gctttgctaa tgtcggcgat atcagccgta
    31141 ttaccgacga tccgcagtgg gaaaacgtct acgtcgagcg cattgttcgc catattcacg
    31201 cgcagaaaaa ccatccgtcg atcatcatct ggtcgctggg caatgaatcc ggctatggct
    31261 gtaacatccg cgcgatgtac catgcggcta aggcgctgga tgacacgcga ctggtgcact
    31321 acgaagaaga tcgcgatgct gaagtggtcg atattatttc caccatgtac acccgcgtgc
    31381 cgctgatgaa tgagtttggt gaatacccgc atccgaagcc gcgcatcatc tgtgaatatg
    31441 ctcatgcgat ggggaacgga ccgggcgggc tgacggagta ccagaacgtc ttctataagc
    31501 acgattgcat tcagggacat tatgtctggg aatggtgcga ccacgggatc caggcgcagg
    31561 atgacaacgg caatgtctgg tataaattcg gcggcgacta cggcgactat cccaacaact
    31621 ataacttctg tcttgatggt ttgatctatt ccgatcagac gcctgggccg ggcctgaaag
    31681 agtacaaaca ggttatcgcg ccggtaaaaa tccacgcgtt ggatctgact cgcggcgagt
    31741 tgaaagtcga aaataaactg tggtttacca cgctggatga ctacaccctg cacgcagagg
    31801 tgcgcgccga aggtgaaacg ctcgcgacgc agcagattaa actgtgcgac gttgcaccga
    31861 acagcgaagc ccccttgcag atcacgctgc cgcaactgga cgctcgtgaa gcgtttctca
    31921 acattacggt gaccaaagat tcccgcaccc gctacagcga agccggacac cctatcgcta
    31981 cttatcagtt cccgctgaag gaaaataccg cgcagcctgt acctttcgcg ccaaataatg
    32041 cgcgtccgct gacgctggaa gacgatcgtt tgagctgcac cgttcgcggc tacaacttcg
    32101 caatcacctt ctcaaaaatg agtggcaaac cgacatcctg gcaggtgaat ggcgagtcgc
    32161 tgctgacccg cgagccaaag atcaacttct tcaagccaat gatcgacaac cacaagcagg
    32221 agtacgaagg gctgtggcag ccgaatcatt tgcagatcat gcaggaacat ctgcgcgact
    32281 ttgccgtaga acagagcgat ggtgaagtgt tgatcatcag ccgcacggtt attgccccac
    32341 cggtgtttga cttcgggatg cgctgcacct acatctggcg catcgctgcc gatggccagg
    32401 ttaacgtggc gctttccggc gagcgttacg gcgactatcc gcacatcatt ccgtgcatcg
    32461 gtttcaccat ggggattaac ggcgaatacg atcaggtggc gtattacggt cgtggaccgg
    32521 gcgaaaacta cgccgacagc cagcaggcta acatcatcga tatctggcgc agcaccgtcg
    32581 atgccatgtt cgagaactat cccttcccac agaacaacgg caaccgtcag catgtccgct
    32641 ggacggcact gactaaccgc cacggcaatg gtctgctggt tgttccgcag cgcccaatta
    32701 acttcagtgc ctggcactac acccaggaaa acatccacgc agcccagcac tgtaacgagc
    32761 tgcagcgcag tgatgacatc accctgaatc tcgaccacca gttgcctggc ctcggctcca
    32821 actcctgggg cagcgaggtg ctggactcct ggcgcgtctg gttccgtgac ttcagctacg
    32881 gctttacgtt gctgccggtt tctggcggag aagctaccgc gcaaagccag gcgtcgtatg
    32941 agttcggcgc agggttcttt tccacgaatt tgcacagcga gaataagcaa tgaggatcat
    33001 cgataactta gaacagttcc gccagattta cgcctctggc aagaagtggc aacgctgcgt
    33061 tgaagcgatt gaaaatatcg acaacattca gcctggcgtc gcccactcca tcggtgactc
    33121 attgacttac cgcgtggaga cggactccgc gaccgatgcg ctatttaccg ggcatcgacg
    33181 ctattttgaa gtgcattact acctgcaagg gcagcaaaaa attgaatatg cgccgaaaga
    33241 gacattacag gtagtggaat attatcgtga tgaaactgac cgcgaatatt taaaaggctg
    33301 cggagaaacc gtagaggtcc acgaagggca aatcgttatt tgcgatatcc atgaagcgta
    33361 tcggtttatc tgcaataacg cggtcaaaaa agtggttctc aaagtcacca tcgaagatgg
    33421 ttatttccat aacaaataaa aactacggcg gcaaaaggag tttgccgcca ccgctaccct
    33481 actcattttc ggagatgtgt tatgtctgat accaaacgta atacaatcgg caaattcggc
    33541 ttgctctcgc tgacttttgc cgccgtttac agctttaaca acgttatcaa taataatatt
    33601 gagcttggac tggcctcggc accgatgttt ttcctcgcaa caatttttta ttttattccc
    33661 ttctgtctga tcatcgcaga atttgtttcg ttaaataaaa actcggaagc cggtgtctac
    33721 gcgtgggtaa aaagttcgct gggcggacgt tgggcattta ttactgccta tacctactgg
    33781 ttcgtaaacc tgttcttttt cacctcatta ttgccgcgcg ttattgctta cgcctcgtat
    33841 gcattcctcg gctacgaata tattatgacg ccggttgcca ccaccattat cagtatggtg
    33901 ctgttcgcct tttccacctg ggtttctacc aacggggcga aaatgctggg gccaattacc
    33961 tccgtcactt caacgctgat gctgctgtta acgctctcct acattttact ggcaggtacg
    34021 gcactggttg gcggcgtaca gcctgctgac cccatcactg ttgacgcgat gatcccaaac
    34081 tttaactggg cgttcctcgg cgttaccacc tggatcttta tggctgcagg tggcgcggag
    34141 tccgtcgcgg tgtacgttaa cgacgtcaaa ggcggttcga aatcgttcgt taaagtgatc
    34201 atcctcgccg ggatttttat tggcgtactg tactccgtct cctcggtact gattaacgtc
    34261 ttcgtcagca gcaaagagtt gaagtttacc ggcggatcgg tacaggtatt ccacggcatg
    34321 gcggcgtatt ttggtctacc ggaagcactg atgaatcgct ttgtcggtct ggtgtccttt
    34381 accgcgatgt tcggttccct gctgatgtgg acggcaacgc cggtgaaaat tttcttctcc
    34441 gaaattccgg aaggcatctt tggtaagaaa accgtcgaac tgaacgaaaa cggcgttccg
    34501 gcgcgcgcag cgtggatcca gttcctgatc gtcatcccgc tgatgattat cccgatgctc
    34561 ggttccaata ccgtgcagga tctgatgaat actattatta atatgactgc tgcagcgtcc
    34621 atgcttccgc cgttattcat catgctggct tacctgaatt tacgcgccaa attagatcac
    34681 ctgccacgcg atttccgtat gggctcccgc cgcaccggta ttatcgttgt ttcaatgctg
    34741 attgcgatat ttgcagtagg gtttgtcgct tcgacattcc cgactggcgc gaatattctg
    34801 accatcattt tttataacgt cggcggtatt gttatcttcc tcggctttgc gtggtggaaa
    34861 tacagtaaat atataaaggg attaacggct gaagagcgcc atattgaagc gacgccagcc
    34921 agcaatgttg attaagcaga ataataaaaa atgagcaggc attttccctc ttcctggatg
    34981 gggagaggga aaatagtttc tgcctttata tttttataat tacaacgata aaaggctgta
    35041 ctttttcttt agctcatgga ttaacacaat gaaattaatc actgcaccat gcagagcatt
    35101 acttgccctg cctttttgct acgctttttc tgcacttggc gaagaagcac gtccggcaga
    35161 acatgacgac acaaaaaccc ccgaaatgat ctcgacatct tctccttcat ttcgttttta
    35221 cggcgaatta ggggttggtg ggtatatgga tttagagggt gagaataaac ataaatacag
    35281 cgacggcacc tatattgaag gtggcctgga gatgaagtac gactcctggt tcggcctgat
    35341 ttacggcgaa ggctggaccg tgcaggccga ccacgacggt aatgcctggg tgcctgacca
    35401 tagctggggt ggtttcgaag gtgggattaa ccgtttctat ggcggttatc gtaccaatga
    35461 tggcaccgaa atcatgctca gtctgcgtca ggattcctcg ctggatgacc tgcaatggtg
    35521 gggcgatttc acgcccgatc tgggctacgt cattcccaat acccgcgaca ttatgactgc
    35581 gctgaaggta cagaacttaa gcggcaactt tcgttatagc gttaccgcca cacctgccgg
    35641 acatcatgac gaaagcaaag cctggctaca ttttggcaag tacgatcgct acgacgacaa
    35701 atacacctat ccggcaatga tgaacggtta catccagtat gaccttgcag aaggtatcac
    35761 ctggatgaac ggtctggaaa tcaccgacgg cacaggccaa ctctatctca cgggcctgct
    35821 tacccctaac tttgccgctc gcgcctggca ccataccgga cgcgccgacg ggctggacgt
    35881 accgggaagt gaaagtggga tgatggtgag cgccatgtat gaagcgttaa agggcgttta
    35941 tctctccacc gcttacacct acgccaaaca tcgccctgac cacgctgacg atgaaaccac
    36001 ctctttcatg cagtttggta tctggtacga atacggcggc ggacgtttcg caacggcttt
    36061 tgatagccgc ttctacatga aaaatgcctc tcacgatccc agcgaccaac tcttcctgat
    36121 gcaatatttt tactggtaat aaggacagta atctcatgaa aatcaaaact attttaacac
    36181 cagtagcctg tgctctgctg atgagctttt ccgcccatgc cgctaacgct gacaattata
    36241 aaaacgtgat taaccgtact ggcgcgccgc agtacatgaa ggattacgat tacgacgatc
    36301 accagcgttt taatccgttt ttcgatctcg gagcctggca tggtcatctg ttgccagacg
    36361 gcccgaacac tatgggcggc tttccaggcg ttgcgttgtt gacggaagag tacatcaact
    36421 ttatggccag caatttcgac cgcctgaccg tctggcagga cggcaagaaa gtcgatttca
    36481 cgctggaggc atacagtatt cccggcgcgc tggtgcaaaa gttgatatcg aaagatgtac
    36541 aggtcgaaat gattctgcgc ttcgccacgc cgcgcacatc actactggaa accaaaatca
    36601 ccagcgataa accgctggat ctggtgtggg atggcgaact gctggaaaaa ctggaagcga
    36661 aagaagggaa accgctttcc gataaaacca ttgctggcga gtaccctgac tatcagcgca
    36721 aaatcagcgc cacccgcgat ggcctgaaag ttacctttgg caaagtgcgc gccacctggg
    36781 atctgctgac ctccggtgaa tcagaatatc aggtgcataa atcattaccg atgcagactg
    36841 aaatcaacgg caatcgcttt accagtaagg cgcatatcaa cggatcgacg acgctctata
    36901 ccacctattc ccatctgctg accgctcagg aagttagcaa agagcaaatg cagatccgcg
    36961 atattctggc gcgtccggcg ttttatctca ccgcctcgca gcaacgttgg gaagaatacc
    37021 tgaagaaagg attaaccaat ccggatgcga cgccggaaca gacgcgcgtc gcggtgaaag
    37081 ccatcgaaac gctcaacggt aactggcgat cgccgggcgg tgcggtgaaa tataacaccg
    37141 tcacgccgtc ggtgaccggg cgctggttct ccggcaacca gacctggccg tgggatacct
    37201 ggaagcaggc gtttgcgatg gcgcatttca atccggacat cgccaaagag aatatccgcg
    37261 cggtcttctc ctggcagatc cagccaggcg accgcgtgcg tccgcaggat gtgggctttg
    37321 tccccgacct gatagcgtgg aaccttagcc ccgagcgtgg cggcgatggc ggcaactgga
    37381 acgaacgtaa taccaaaccc agccttgccg cctggtcagt aatggaagtg tataacgtca
    37441 cccaggataa ggcctggttg gcagagatgt acccgaagct ggtggcctat cacgactggt
    37501 ggttacgtaa ccgcgatcac aacggcaacg gcgtgccgga atatggcgcg acccgcgaca
    37561 aagcccacaa caccgagagc ggcgagatgc tgtttacggt gaaaaaaggc gacaaagagg
    37621 agacgcagtc tgggctgaac aactacgccc gtgtggtgga gaaaggccag tacgacagtc
    37681 tggaaattcc ggcacaggtt gctgcgtcgt gggaatcagg tcgtgatgac gccgccgttt
    37741 tcgggtttat cgacaaagaa cagctggata aatatgtcgc taacggcggc aaacgtagcg
    37801 actggacggt gaaattcgcc gaaaaccgca gtcaggacgg aacgttgctg ggctactcgc
    37861 tattgcagga gtcggtggat caggccagct atatgtacag cgataaccat tatctggcgg
    37921 agatggcgac gattctcggt aagccggaag aggccaaacg ctatcgtcag ttagcacaac
    37981 agctcgcgga ctacatcaac acttgtatgt tcgacccgac tacacagttc tactatgacg
    38041 tgcgtattga agataaaccg ctggcgaacg gctgcgcggg caaaccgatt attgagcgcg
    38101 gtaaagggcc ggaaagctgg tcgccgctgt ttaacggtga ggcaacgcag gccaatgccg
    38161 acgcggtggt gaaggtgatg ctggatccga aagaattcaa cacctttgtc ccgctgggaa
    38221 cggcggcgtt aaccaatccg gcttttggcg cggatatcta ctggcgcggg cgcgtatggg
    38281 tggatcagtt ctggtttggt ctgaaaggga tggagcgtta cggttatcgt gatgatgccc
    38341 tgaagctggc ggatacgttc ttccggcacg ccaaaggatt aaccgccgat ggcccaattc
    38401 aggagaatta caacccgctc actggctcgc aacaaggcgc accaaatttc tcctggagtg
    38461 ccgcgcattt gtatatgttg tataacgatt ttttccgtaa gcagtaattt gcaacggctg
    38521 gcggatgcgg cgtgaacgcc ttatccgccc tacatgtgca atcccccagg ccggataaaa
    38581 cgcggcaagc gtcgcatccg gcgttaccac cgggcgtatt ctttttgaat cccatcacaa
    38641 accccgcact ccccttttcc cttttctccg gcgacggcta aattagaact catccgacca
    38701 cataacaatt attttacata ctggacaatt ttatgagcta cccgtcgctg ttcgccccgc
    38761 tggatttagg ttttaccacg ttaaaaaacc gcgtgttgat gggctcaatg cacaccgggc
    38821 tggaggagta cccggacggc gctgaacggc tggcagcgtt ttatgccgaa cgcgcccgtc
    38881 acggcgtggc gttgattgtc agcggcggta tcgcaccaga tttaacaggc gttggcatgg
    38941 aaggcggtgc aatgctcaat gacgccaacc agatcccaca ccatcgcacc attaccgaag
    39001 ccgtacatca ggaaggcggc aaaatcgccc tgcaaatttt gcataccggg cgctacagct
    39061 accaaccgca tctggtcgct ccctccgcat tgcaggcccc catcaaccgt ttcgttcctc
    39121 acgaactcac ccatgaagag atcctgcaac tgatcgacga tttcgcccac tgcgcgcaat
    39181 tggcacggga ggcaggatac gacggtgtag aggtgatggg gtccgaaggg tatttgatca
    39241 acgaatttct gacgctgcgc accaatcagc gtagtgacca gtggggcggc gattaccgca
    39301 accggatgcg gtttgccgta gaagtagtgc gtgcggtgcg cgaacgtgtc ggcaacgact
    39361 tcattattat ctaccgtttg tcgatgctcg acctggtcga aggcggcggt acttttgccg
    39421 aaatggtaga gctggcgcag gccattgaag cggcgggcgc gaccattatc aacaccggca
    39481 ttggctggca tgaagcgcgg attccgacca tcgccacgcc cgtgccgcgc ggcgccttta
    39541 gctgggtcac gcgcaaactg aaagggcacg tttcgctgcc actggtgacc accaaccgga
    39601 ttaacgatcc gcaggttgcc gacgacattc tctcgcgcgg tgatgcggac atggtatcga
    39661 tggcgcgacc gtttctcgct gatgcggagc tgctatcaaa agcgcaatcg ggacgagccg
    39721 atgagatcaa cacttgtatt ggctgcaacc aggcctgtct cgatcagatc ttcgttggca
    39781 aagtcacctc gtgcctggtg aatcctcgcg cctgccacga aaccaaaatg ccaatccttc
    39841 ctgccgtgca gaaaaaaaat ctggcggtgg tcggtgcggg acctgctggg ctggcgtttg
    39901 ccattaacgc ggcggcgcgt gggcatcagg taacattgtt tgatgcgcac agcgagatcg
    39961 gcgggcagtt taatatcgcc aaacagatcc ccggcaaaga ggagttttac gaaacgctgc
    40021 gctattaccg ccggatgatc gaagtgacgg gtgtaacgct aaaactcaat cacaccgtga
    40081 cggcggatca gttacaggcg ttcgatgaaa cgatcctcgc cagtgggatc gtgccgcgcg
    40141 ttccgcctat cgacgggatc gatcatccga aggtactgag ttatctcgat gtactgcgcg
    40201 acaaagcgcc ggtgggcaac aaagttgcca tcatcggttg tggcggaatt ggttttgata
    40261 cggcgatgta tttaagtcag ccgggcgaat ccaccagcca gaatatcgcc gggttctgta
    40321 atgagtgggg aatcgacagt agcctgcaac aggccggagg tttgagtccg caaggcatgc
    40381 aaatcccccg tagcccacgg cagattgtga tgctccagcg caaagccagc aaaccaggac
    40441 aagggttagg caaaaccacc ggctggatcc atcgcaccac cctgctctca cgcggcgtga
    40501 aaatgatccc aggcgtaagt taccagaaga tcgacgatga cgggctgcat gtagtgatca
    40561 acggcgaaac gcaggtacta gcagtggaca atgtggtgat ctgcgcgggg caagagccaa
    40621 accgcgcatt ggcgcaaccg ctgattgata gtgggaaaac tgtgcattta attggcggct
    40681 gcgatgtagc tatggagctg gacgcacggc gggcaattgc ccagggaaca cggctggcgc
    40741 tggagattta aatcgttgtt tgaagcgccg gatgcgacgc atccggcacg tcgcgttaat
    40801 caataaacaa ggcgggagaa ataccgaatc gcgttgccag ttttttagcg tgttccagcg
    40861 ttaatttcct tttcccgctc aaaacgcgtg acaccatcga tttactgcct atttccggaa
    40921 gatcggaaag ggttaaacca tattgatcca taagggtacg aatcacggct ataccgccag
    40981 gcatggcttg agccatggca ttaaattcca caaattcggg cgctgattct tcccacgcgg
    41041 ttattttggc acacaccaga tccagtaagg ggttttcagg atcgttgagc agcagatgat
    41101 ctaccagttc cagcgcctgg gtgtattgtt cctcgttctg aatacccgcc agaaaaggtg
    41161 ccacagcagt tagcttttct cctgcttgca agatgtcggc aatcgcaatc attttttccc
    41221 cttagtacga tgaacggcgg taaagaaatc gtattcttta tgcgtcataa cttcacgtat
    41281 gtagcacttt tgcgattcaa aaaagaccat tgctacaaca cgtaattcat tgcccccgac
    41341 attgaaaaca taatgcttat ccagatattt gaagttatcc agagaaggga atactgcttt
    41401 taatgactca ggttttttga aatatccctt agcaatcgtg ttccccagag ccaccaactc
    41461 cgttttatgt tgcgggtatt tttccgcagc atctttcaat gctttttgag ttatcaggtg
    41521 cattcttcat cacgtccgtt gacaaattgg caatatgata acatccgttg acagattggc
    41581 aacgaatgaa tttatctgtg tgacggaaga ataaacgtag aaaaacgcta aacaagagaa
    41641 tgtagaggat ggtgacagga attttttgtt ttatggaagc gcgattccag aactgggcag
    41701 acattgccgg atgcgacgct tgccgcgtct tatccgacct gggggtaatg tgctgaacgt
    41761 atgccggata aggcgtttac gccgcatccg gcaggtaacc ctcaatctta ccgacgacgc
    41821 cccagcttca ctgctttcag caccacaaat ttattattcg tggcgatggt ggtgcagttg
    41881 ccgaaaattt tcttcagttt atggaagtaa tccaagtgac ggttggcaac gatatacagc
    41941 tcgccgttga ttttcaggca gcggcgggcg tggtggaaca tctcccaggc gacgttatcg
    42001 gtcagcgcat gttgctgatg aaacggcggg ttgcagagca cagcattaaa acggaaaggc
    42061 tccacgccgg aaagcgcgtt gttgatcata aactcgcagc gatccagtgc ctcgggcatg
    42121 ttggtttcaa cgttcaaacg gctggaagca accgccatcg gtgattcatc gacaaacacc
    42181 actttcgcct gcgggttttt atcgagcaat gtcaggccaa taacgccatt accgcaaccg
    42241 agatcgacaa tctcaccttc gagattctct ggcagatgtt gcataaagaa gcgcgcgcca
    42301 atatccagcc cggtgcggga gaagacattc gcatggttgt ggatagtcca gtcagtacct
    42361 tccagcttcc agctaacggt ctgcggcgca tcggccaacg gcggttcgtt gaaggtgcag
    42421 ttaatcaggc gcgctttctt ccatgccagc gtggtggtgg tcgggccgag cactttttcg
    42481 aacaattcca gcgtggaagt gtgaatgtca cgggctttgg caccagcaat aatgcgcgta
    42541 tctggcgtga ccactttgcg cagcgcgcgc agttgttgtt ccagcaatgc cagtgttttc
    42601 ggcactttga tcagcaccac acccggctgt tgcgggtagt cggcggtgct gtcgagaaac
    42661 ttcacgctcg actcatcaat cccgttgagg cgtaaattct cgcgcgtggc cagttcactg
    42721 atgtatgagt cgccaatgct gtacggctta tgttccgcca gcacacagct taacgcacca
    42781 aaagcatcat tcaggatcaa caccgggccg cggatttctg tgtcgtccaa ctgttgcagc
    42841 aaatactcat ccgccgcttc ccacgcctgt agcgggttaa cgtcatccgt cgccggaaaa
    42901 cgttgtagtg tcagtgaacg gaaaccgttg tctaagtggc tcatcggccc tcctgaatga
    42961 taaaatttcg acgttgcccc tgaaaagggt gcgtgagtat accctttttc tctttttcgt
    43021 gggcggttat gagcaatctt acttatcttc agggttatcc cgagcagcta ctttcccagg
    43081 tgcgaacgct gattaacgaa cagcgtctgg gcgatgtgct ggcaaaacgt tatccgggaa
    43141 cacacgacta cgccaccgat aaagccctct ggcaatatac tcaggatctt aaaaatcagt
    43201 ttctgcgcaa tgccccgccg atcaataaag tgatgtatga caataagatc cacgtgctga
    43261 aaaacgcgct cgggttacat actgccgttt cgcgtgtgca gggcggcaag ctgaaagcaa
    43321 aagcagagat ccgcgtcgct accgtgtttc gcaacgcgcc ggaaccgttt ttgcgcatga
    43381 tcgtggtgca cgagctggcg cacctgaagg agaaagagca taacaaagcg ttttaccagt
    43441 tgtgctgtca tatggaaccg cagtaccacc agcttgagtt cgacacccgg ctgtggctaa
    43501 cgcagttgtc gcttgggcag gacaaaatct gaaaatgatg tcaggcgtta ttttttcctc
    43561 ctttagcgcc taaaatcgat ctcctccctt tcgttaagga taacgaccat gctgcgcgca
    43621 tttgcccgcc ttcttcttcg tatttgtttt tctcgccgga ctcttaaaat tggctgttta
    43681 ttgttgctta tagcaggagc gaccattttt attgccgatc gcgttatggt caacgccagc
    43741 aagcagctga cctggagtga tgtcaacgcc gttccggcgc ggaacgtggg tttattgctg
    43801 ggggcgaggc cgggtaatcg ctactttacc cgacgtattg atagcgccgc agagttgtac
    43861 cacgccggaa aagtgaaatg gctattggtg agcggcgata atggacgtaa aaattacgat
    43921 gaagcgtcag gaatgcagca agccttgatc gctaaaggcg taccggcaaa agccatcttc
    43981 tgcgactatg ccggattctc aacgcttgat tcggtggtgc gtgccaacaa ggtgtttagt
    44041 gaaaaccata tcaccattat ctcgcaagaa tttcataacc aacgcgccat ctggctggca
    44101 aaacagtatg gtatcgatgc tatcggtttt aacgcgccag acctgaatat gaagcacggt
    44161 ttatatactc aactgcggga aaaacttgcc agagtcagcg cggtgattga tgcgaaaatc
    44221 ctccatcgcc agccgaaata tctcgggccg ttggttatga ttgggccgtt tagtgagcat
    44281 ggctgtccgg caaaagaata atgcgtatct gcgcacgtcg aagataaaaa aggcgtgcta
    44341 cagtgacgac agaatccctt tatggagtat ccacgcgtta tgatacgttt cgctgtgatt
    44401 ggtacgaact ggatcactcg ccagttcgtc gaggccgccc atgagagcgg taaatacaag
    44461 ttaaccgccg tctattcccg cagccttgaa caggcccagc acttcgccaa tgatttttct
    44521 gtcgagcatc tgtttacgtc gctggaagcg atggcagaaa gcgatgccat tgacgcggtg
    44581 tatattgcca gcccaaattc cctgcacttt tcccagacac aacttttcct tagtcacaaa
    44641 attcatgtga tttgcgagaa gccactggcg tcgaatcagg cggaagtgga tgccgccatt
    44701 gcctgtgcgc gggaaaatca ggtggtgctg tttgaggcat ttaaaacggc ctacctgccg
    44761 aactttcatc tgttacgcca ggcgctgccg aaagtcggca aactgcgtaa agtctttttc
    44821 aactattgcc agtattcctc gcggtatcag cgttacctgg acggagagaa tcccaacacc
    44881 ttcaatccgg cattctccaa cggttcgatt atggatatcg gcttttactg cctggcgtcg
    44941 gcggtggcat tattcggtga accgaaaagc gtgcaggcaa ccgccagttt gctggcaagc
    45001 ggtgtggatg cccacggcgt ggtggtgatg gattacggtg atttcagcgt tacgttgcag
    45061 cactcaaaag tgagcgattc tgtcctggca agcgagattc agggcgaagc gggatcgctg
    45121 gtgattgaaa aactctctga atgccagaaa gtctgctttg taccgcgtgg cagccagatg
    45181 caggatctca cccagccaca gcatattaat accatgctct acgaagcaga gctgttcgct
    45241 accctggtgg atgagcatct ggtgaatcat ccagggctgg cggtcagtcg tatcaccgcc
    45301 aaactgctga ctgagatccg ccgccagact ggggtgattt ttccggcaga taacgtaaaa
    45361 ctataattgc caaagtaaaa cagtgtaaaa ggtatgtaac agaccattga ctgactgaat
    45421 ggtctgtcat actttgttac ctgcaaaggg gagtaacttc attgtcggtc gatcgtcatt
    45481 acggtgtgtg aaaaaccaca tccggtcacc gggcaacccg aaaggaatac gcagacgtat
    45541 tccttttttg ttgtaagtga gaccttgccg gaaggcgagg tctatgcata aaaagcagcg
    45601 gctgacgtct tccgacgttg gccgtttttt tatgtgtaag gaacttctat gaatactgtc
    45661 ggcacgccgt tgctatgggg cggattcgct gttgttgtca ccattatgct ggctatcgac
    45721 ctgttgttgc aggggcgtcg tggggcacat gccatgacca tgaaacaggc tgcggcctgg
    45781 tcgctggtct gggtgacgct gtcgttactg tttaacgccg ctttctggtg gtatctggtg
    45841 caaaccgaag gtcgcgccgt tgccgatcca caggcactgg cctttctcac gggttatctg
    45901 attgagaaat cgctggcggt cgataacgtc tttgtctggc tgatgttgtt cagctatttc
    45961 tctgttccgg cggcattaca acgccgcgtg ctggtgtatg gcgtactcgg ggcgattgtt
    46021 ctgcgtacca tcatgatctt cactggtagc tggctgattt cacagttcga ctggatcttg
    46081 tatatcttcg gtgccttcct gctgtttacc ggcgtgaaga tggcgctcgc ccatgaagat
    46141 gaatcaggca ttggcgacaa gccgctggtg cgctggctgc gcggtcattt gcgcatgacc
    46201 gacaccatcg acaacgagca tttctttgtg cgtaagaatg gtttgttgta tgccacgccg
    46261 ctgatgctgg tgctgattct ggtggagttg agcgacgtga ttttcgccgt ggatagtatt
    46321 ccggctatct tcgccgtgac caccgatccg tttattgtgc tgacctcaaa cctgtttgcg
    46381 atcctcggcc tgcgtgcgat gtatttcctg ctggcaggtg tggcagagcg tttctcgatg
    46441 ctcaaatatg gtctggcggt gattctggtg tttatcggta tcaagatgct gattgtcgac
    46501 ttctaccata ttccgatcgc cgtctcgctg ggcgtggtgt ttggcattct ggtgatgacg
    46561 tttattatca acgcctgggt gaattatcgg catgataagc agcgggttgg atgattttta
    46621 atctgcctaa gccgtgtacc ctgtcatcca catgagcacc gttttctccc tctccccaaa
    46681 aggggcgagg ggactgaccg agtacttttt tgacgttgtc atcagtctgg aagccgcacg
    46741 ttgggcttta ttttcatgtc aaacaaatgt aaccattaag ttgcgaaata tgacttctcc
    46801 ttaaaatcca gcatatttcg cttcccgaag ctgtaacttt ccttatactc gaccttgcaa
    46861 acactttgtt acatcctgaa agatgcgtcg acagaacgca ccagggatgt gcgacaacac
    46921 aatgaaagga tcgaaaaatg actacgcaac attcaccggg gctattccgg cgtctggctc
    46981 atggcagcct ggtaaaacaa atcctggtcg gccttatact ggggattctt ctggcatgga
    47041 tctcaaaacc cgcggcggaa gctgttggtc tgttaggtac tttgttcgtc ggcgcactga
    47101 aagccgttgc ccccatcctg gtgttgatgc tggtaatggc atctattgct aaccaccagc
    47161 acgggcagaa aaccaatatc cgccctattt tgttcctcta tctgctgggc accttctctg
    47221 ctgctctggc cgcagtaatc ttcagttttg ccttcccttc taccctgcac ttgtccagta
    47281 gcgcgggtga tatttcgccg ccgtcaggca ttgtagaagt gatgcgcggg ctggttatga
    47341 gcatggtttc caaccccatt gatgcgctgc tgaaaggtaa ctacatcggg atcctggtgt
    47401 gggcaattgg cctcggtttc gcactgcgtc acggtaacga aaccaccaaa aacctggtca
    47461 acgatatgtc gaatgccgtc acctttatgg tgaaactggt gattcgcttc gcaccgatcg
    47521 gtatttttgg tctggtttct tctaccctgg caaccaccgg tttctccacg ctgtggggct
    47581 acgcgcaact gctggtcgtg ttggttggct gtatgttact ggtggcgctg gtggttaacc
    47641 ccttgctggt gtggtggaaa attcgtcgta acccgttccc gctggtgctg ctgtgcctgc
    47701 gcgaaagcgg cgtgtatgcc ttcttcaccc gcagctctgc ggcgaacatt ccggtgaata
    47761 tggcgctgtg tgaaaagctg aatctggatc gcgataccta ttccgtttct attccgctgg
    47821 gagccaccat caatatggcg ggcgcagcaa tcaccattac cgtgttgacg ctggctgcag
    47881 ttaatacgct gggtattccg gttgatctgc ccacagcgct gctgttgagc gtagtggctt
    47941 ctctgtgtgc ctgtggcgca tccggcgtgg cggggggatc tctgttgctg atcccactgg
    48001 cctgtaatat gttcggtatt tcgaacgata tcgccatgca ggtggttgct gtcggcttta
    48061 ttatcggcgt attgcaggac tcctgtgaaa ccgcgctgaa ctcgtcaact gacgtgctgt
    48121 tcactgcggc agcttgccag gcggaagacg atcgtctggc aaatagcgcc ctgcgtaact
    48181 aatacttagc ccctttcgtc tacggcggaa ggggttttct ccactttaaa cggatcaatt
    48241 ccccttctct gcatacgcca gaaacgaatg atattcaggc cattcatcag cagaaaactg
    48301 ccttcaatca tcgtgccgcc tatcgacccc gcccagaagt tgtgaatcac ccagcaacac
    48361 gttgaaaacc acattacgca gcgcatggtc agccctttac agcggaacag cgcccaggta
    48421 ctgacaatcg tgccgattac cggcaatagt tcaacaggat gatggaactt cgcgaggcca
    48481 attccgccag tcagcacaat aaaaatcgcc attacccata agctgcgcgt gcgtaaggta
    48541 atcaatgtac gaatggcatt aaggatggca ctggcaccgg caggataggt gcccagaaga
    48601 aaaaaatgta cgccaataac ggcgctatag accgaaagtt gctttttgaa gcgacgttcg
    48661 tcacgattga aaaatgttgt gataccaatc agaaaggcga tgacacccac gccctgggcc
    48721 agccaatacg cggtcatgat aaatccttag caggtatgga aaagcaaacg gcgcttcaca
    48781 ttatgaaacg ccgtttttta ttaacaactc atttcgactt tatagcgtta cgccgctttt
    48841 aaagatcgcc agttcgcgga agtcgttacg ctcgttacag gtttgcttac cgttggcaaa
    48901 ctcaacgatg gtgtcgataa attcttccag caactgcggc atcgctttac cgtggatcag
    48961 ctgacccgcg tcaaagtcga tccagtgttt tttcttcgcc gccagttcac tgttggtggc
    49021 gattttcacc gtcggcacaa atccaccata cggcgtgcca cgaccagtac tgaacagcac
    49081 catatggcag cccgcacccg ccagggcgct ggtcgctacg gcatcgttac ccggcgcact
    49141 taacaagttc agccccggcg ttttcagacg ctcgccgtaa cgcagcacgt caaccacgac
    49201 actggaaccc gctttctggg tacagccaag cgatttatct tccagcgtgg tgataccgcc
    49261 tgctttgttc cccggtgatg ggttttcgta gatcggctgg tcatgggcaa taaagtactg
    49321 cttgaagtca ttgaccatgg tgaccagttt ttcaaacgtt gcttcgtcgc ggcaatggtc
    49381 catcagcaac tgctctgcgc caaacatctc cggcacttcg gtcagtacgg tagtaccgcc
    49441 gttagcaatc acgtagtcag agaaacgccc cagcatcggg ttagcagtaa taccagaaag
    49501 accgtcagaa ccaccgcact ccagaccaaa cttcagttcg ctgagtttgc ccggctcgcg
    49561 tttatcgttg cgcatcacgc tatacagctg atgcaaatgc gcgatcccgg cctcgatttc
    49621 atcatcctgc tgttggcaga tcatgaaatg aacgcgttca ggatcgatat cgcccaacgt
    49681 ttcacggaat gcggcaacct ggttattttc gcaacccaga ccaatcacca gcactgcgcc
    49741 cgcgttcggg tggcgcacca tgttttgcag catggtgcgg gtattgatgt gatcgtcgcc
    49801 cagttgtgag cagccgtagg tgtggctgaa gaggaacacg ccgtcggtac cttcagcgtt
    49861 gttggtctct ttcaggaaac gattttggat ctgccgcgcg atgccgttga cacagcccac
    49921 ggttggcagg atccacagct cattacgcac cccgacatca ccgttagcgc gacgatagat
    49981 ctgcacttca cgatctgccg cttgcgcagg cagatcctga aaatcaggtt gatagcgata
    50041 ctgatccaga tcgctcagat tcgtgcgcgt attgtgggcg tgaacgtgtt cccccgccgc
    50101 aatatccgcc aatgcataac caatcggcag gccatattta atgacgttgg ccccttttgc
    50161 gatatccgtt aacgcaaatt tatgtccacg agcaacatcc tggcgcagcg taacagtctg
    50221 gttatcgaca ctgacttctg tgccttcagc caaatccgct aaagcaaccg cgacgttatc
    50281 cagcgcatgg atcttgatgt attgcatatc aaccccagac cttagttcag ttcaatggcg
    50341 aagtagtcac gcgcattgtt aaagcaaata tttttcacca tctcgcccag caggttgatg
    50401 tccgccggtg cttcgcctgc ttccacccag cgaccgatca tctggcacag aatgcggcgg
    50461 aagtattcgt gacgggtgta tgacaggaag ctacggctgt cagtcagcat gccgacaaag
    50521 cggctcagca gaccgagctg cgccagttgg gtcatctgac gttccatacc gtctttctgg
    50581 tcgttaaacc accagccgga accgaactgc attttgcccg gcataccttc gccctggaag
    50641 ttaccgatca tggtgcccag cacttcgtta tcgcgcgggt tcaggcagta gagaatggtt
    50701 ttcggcagca ggttttcttc gttctgcttg ctcagcagct tagatagctc ttccgccatc
    50761 ggacggtcgt tgatggagtc aaagccgaca tccggcccca gcagtttgaa ctgacgcagg
    50821 ttgttattac gcagtgcgcc aatgtggtac tgctgtaccc atccgcgacg tgcatattcg
    50881 gcaccaagga acaccagcac cgcagttttg aactgtgcta cttcgtgctc gctcagggtt
    50941 tcgcctgcca gacggcgcgc gaggatgctg tccagttccg cttcgttcgc ttcagcaaac
    51001 atcaccacat ccagcgcgtg gtcagacact ttacagccgt gagcggcgaa gtgatccaga
    51061 cgtttagtca gggcagtttg caggtcagca aagcggcgaa tgtcggtatc agaaacttcg
    51121 cccagcttcg ccatgtagtc gttaaaggtc gcctgttcaa tgttaaaggc tttgtccgga
    51181 cgccagctcg gcagcacttt aatggtgaaa gagccgtctt tggcgatctc tgcgtgatgc
    51241 tccagagaat cgatcgggtc atcggtggtg ccgaccattt tcacgttcat ctgctgcatg
    51301 atgccgcgcg cggagaagtt atcctgcgcc agcagttcgt tacattcgtt ccagatttca
    51361 tcggcagttg acggagaaag caatttgcca gtgataccaa acggacgacg cagttcgagg
    51421 tgcgtccagt ggtataacgg gttgccgata gtgtgcggaa cagtcgccgc ccaggcgtca
    51481 aatttttcac ggtcagacgc atcaccggtg cacagacgct cggccacacc gttggtacgc
    51541 atagcgcgcc atttgtagtg atcgcccttc aaccagatgt catacaggtt tttaaaacga
    51601 tagtcttccg caatctgctg cggcggcaaa tggcaatggt aatcgaaaat cggctggtct
    51661 tttgcgtagt cgtgatacag acggcgggca aattcagtat ctaacaggaa atcttcagtc
    51721 ataaacggag tcattttcgt cttcctctca acgagtgagc tagcttgctt atggtgcgat
    51781 gctgacaaag ttatcacacc aatttccaga gtccgaagat attttcgtga gttagatcaa
    51841 taaacgtagt taaaaaaatt actctcaaag tggtaaatct cgctgcaggc cgcgccagta
    51901 ctggcctcgt cgtcgtcagg taatgtccct acaaatattc gcacatttgt gatggctctc
    51961 accttttaaa gttgtatgac aagttatctt tctgccgtcg caaatcataa gtcgacggaa
    52021 tgcaaattgc cgattcattc atttgttaga tgaatcgggt taaccggtac ggaagccgaa
    52081 ttagcacgaa actttcatgg caacgttcgg ggcgtgccgg ttttttttcg gttacccggt
    52141 cgtaactaac atcttcagcc tctggcggga tgatggccgc gcttcctgcg gatataacaa
    52201 aacgatgagg ttttacatgc gtaaaattaa agggttacgt tggtatatga tcgcactggt
    52261 gacgctcggc accgtgcttg gttacctgac gcgtaacact gtggcggcag ctgcgccaac
    52321 tctgatggaa gagttaaaca tctccaccca acagtattcc tatatcatcg cagcgtattc
    52381 tgctgcttat acggtcatgc aaccggtagc gggttatgta ctggatgtgc tgggtacgaa
    52441 aatcggttat gcaatgtttg ctgtactgtg ggccgtgttc tgtggtgcaa ccgcgctggc
    52501 aggtagttgg ggtggcctgg cggttgctcg tggtgcggtc ggtgccgcgg aagccgcgat
    52561 gattccggcg ggtctgaaag ccagctccga atggttcccg gcaaaagagc gttccatcgc
    52621 ggtaggttac tttaacgtag gttcttcgat tggtgcgatg attgcgccgc cgctggtggt
    52681 atgggcaatc gtgatgcaca gctggcagat ggcatttatc atctccggtg cgttgagctt
    52741 tatctgggcg atggcatggc tgattttcta caagcatccg cgcgaccaga aacatctgac
    52801 cgatgaagaa cgcgactaca ttattaatgg tcaggaagcc cagcaccagg ttgacacagc
    52861 gaagaaaatg tccgttggtc agatcctgcg taaccgtcag ttctggggta tcgcgctgcc
    52921 gcgcttcctg gcagaaccgg catggggtac ttttaacgcg tggatcccgc tgttcatgtt
    52981 taaagtttac ggctttaacc tgaaagagat cgcgatgttt gcctggatgc cgatgctgtt
    53041 tgctgacctc ggttgtatcc tcggtggtta cctgccacca ctgttccagc gttggtttgg
    53101 tgtgaacctg atcgtttccc gtaagatggt cgtaacgctg ggtgcggtgc tgatgattgg
    53161 cccgggtatg atcggtctgt tcaccaaccc gtatgtcgcg attatgctgc tgtgtatcgg
    53221 tggttttgct caccaggcac tgtctggcgc actgattacg ctctcgtctg acgtgttcgg
    53281 tcgtaacgaa gtggcgacgg cgaacggttt aaccgggatg tccgcatggc tggcaagtac
    53341 gctgtttgca ctggtagtcg gtgcactggc tgacaccatc ggcttcagcc cgctgttcgc
    53401 agttctggca gtgttcgacc tgttaggtgc gctggttatc tggaccgtgt tgcagaacaa
    53461 accagcgatt gaggtggcgc aggaatcaca taacgatccc gcaccgcaac attaaggctt
    53521 cgtgtaagtt ttagtttccc ttcaaagccg ccttctcagg cggctttttc atcactgcga
    53581 gtagagctaa actcgcaaaa gtggtataac aaatatagtc tgccgtatca tgcctggagc
    53641 gcatatggaa atcactgaac cacgccgttt gtatcaacaa cttgccgctg atctgaaaga
    53701 gcgcatcgaa cagggcgtct atctggtggg tgataaactg cctgccgaac gctttattgc
    53761 cgatgaaaaa aacgtcagcc gcaccgtggt tcgtgaagcc atcatcatgc tggaagttga
    53821 aggctatgtc gaagtgcgta aaggttcagg tattcatgtg gtttccaacc agccgcgcca
    53881 tcagcaggcg gctgacaata atatggaatt cgccaattac ggtccgtttg agttgctcca
    53941 ggctcgccag ctcatcgaaa gtaatattgc cgagttcgcg gcaactcagg tcacaaaaca
    54001 ggacatcatg aaattgatgg ccattcagga gcaggcacgc ggcgaacaat gcttccgtga
    54061 ttccgagtgg gatttgcagt tccatattca ggtagccctg gcgacgcaga actccgccct
    54121 ggcggctatc gttgaaaaaa tgtggaccca gcgtagtcat aacccgtact ggaaaaaact
    54181 gcacgaacac attgatgccc gtaccgtcga taactggtgt gatgaccacg atcaaatcct
    54241 caaggcgctg attcgcaaag atcctcatgc tgctaagctg gcaatgtggc agcacctgga
    54301 aaacaccaag atcatgttat ttaacgaaac cagcgacgat ttcgagttca atgccgaccg
    54361 ctatctgttc gccgaaaacc cggtggtgca tctcgataca gccaccagcg gcagtaaatg
    54421 aaattcctgt ccgacaggcg ctacagtgcg cctgtttgtc tcgtcggtaa gcgaaaagta
    54481 taaagtgtca gcctgtgtaa atcctctcgc ctccctcccc tgcattcagc aaaatcagac
    54541 tccatggaca tgtaattttg ataacgaaca acgttgacct ttgttacaat tagattcaat
    54601 ttgaatttat gcttttgaat gctttcttat ctcacgattt aacagggaat agttcaggct
    54661 gtgttgatgt atcaaacccg cagaacatac caagacagca ataacattgc ggtagtgcat
    54721 catttaaaac cagcgtggcg ttaaccgatt caccaggaat aatgaatgga acttttgacc
    54781 caattgctgc aagccctgtg ggcgcaggat tttgaaaccc tggccaatcc gtcgatgatt
    54841 ggcatgttgt attttgtctt gtttgtcatt ttgttccttg aaaacggctt gcttccggcg
    54901 gcctttttac cgggcgacag tttactggta ctggtcggcg tattgattgc gaaaggcgcg
    54961 atgggctatc cgcaaacgat tctgctgctg accgttgccg ccagcctcgg ttgctgggtc
    55021 agctatattc aggggcgatg gctgggcaat acccgcaccg tacaaaactg gctatctcat
    55081 ttacccgcgc attatcatca acgcgcacac catctttttc ataaacacgg tttatcggcg
    55141 ctgttaattg gtcgctttat tgcgtttgtc agaacactgc tgccgacgat tgccgggtta
    55201 tcagggctga ataacgcgcg ctttcagttc ttcaactgga tgagtggtct gctgtgggtg
    55261 ttgatcctga caactctggg ttacatgctc ggcaaaacac cggtattttt aaagttcgag
    55321 gaccagctga tgtcatgcct gatgctgctc ccggtggtgc tgctggtgtt tggcctggca
    55381 ggttctctgg tcgtgttatg gaaaaagaaa tatggaaatc gggggtaagg gatgcaaata
    55441 cctcgcatgt cacttcgcca gctagcctgg tccggcgctg ttttacttct ggtcagcaca
    55501 ctattgctgg cctggtccgc ggttcgccag caagagtcta cgctggcgat tcgtgccgtt
    55561 catcaaggga caacgatgcc agacggtttt tcaatctggc atcaccttga cgctcacggc
    55621 attcctttca aaagtatcac ccccaaaaac gacactctgt taattacctt tgactccagc
    55681 gaccagagcg ccgccgcaaa agcggtcctc gacagaacat tgccccatgg ctacatcatt
    55741 gcgcagcagg acaataacag tcaggctatg cagtggctga cccggttgcg ggataactct
    55801 catcgcttcg gataacttcc tgttatccga aacagatcac tcactttggt gatttcaccg
    55861 taactgtcta tgattaatga ggcggtggtt aacacgaccg cttcgttgtc tggacaatcg
    55921 ttcctttgta ataggtccga atcacaatgg aaggttcaag aatgaaatac cgcatcgctt
    55981 tagctgtttc tctctttgct cttagtgccg gtagttatgc cactaccctg tgtcaggaaa
    56041 aggagcaaaa tatccttaag gagatcagct atgccgaaaa acaccaaaac cagaatcgta
    56101 ttgacggtct gaataaagcc ctgagtgaag tccgggccaa ctgttcagat agccaactgc
    56161 gtgccgatca tcagaagaaa atcgcaaagc agaaagatga ggtggcggaa cgccagcaag
    56221 atttagccga ggcgaagcaa aaaggcgatg cagataagat tgccaaacgc gaacggaaac
    56281 tggcagaagc gcaggaagag ctgaaaaagc tggaagcgcg cgactactaa ctcacaatag
    56341 tcactactta ctcacctgga gaaaactatg tcgaaagaac acactacgga acatctgcgt
    56401 gctgagttga aatccctttc cgatacgctg gaagaggtgc ttagctcatc tggcgagaag
    56461 tcaaaagaag agttgagtaa gattcgtagc aaagcggagc aggcactgaa acagagccgt
    56521 tatcgcctgg gtgaaaccgg tgatgccatt gccaaacaaa cccgtgtcgc ggcggcgcgt
    56581 gccgatgagt atgtgcgtga aaatccgtgg acgggcgtgg gcattggcgc agcaatcggt
    56641 gtagtgctcg gcgttctgct gtcgcgtcgt taattatggc ggacactcat cacgcgcaag
    56701 ggcccggtaa aagcgttctg ggcatcgggc agcgaattgt ttccatcatg gttgaaatgg
    56761 tggagacacg tctgcggctg gcggtggtgg agctggaaga ggaaaaagcg aatctctttc
    56821 aacttttact gatgctgggc ctgacgatgc ttttcgctgc atttggtctt atgagcctga
    56881 tggtgctaat tatctgggcg gttgatccgc aatatcgcct gaatgcgatg attgccacca
    56941 ccgtggtgtt gctgctactg gcactgattg gcggcatctg gacgttacgt aaatcgcgta
    57001 agtctacgtt gctgcgccac acgcgccatg agctggcaaa cgatcggcag ctgctcgagg
    57061 aggagtcccg tgagcagtaa agtcgaacgt gaacgacgta aggcgcaact gcttagccag
    57121 atccagcaac aacggctgga tctttccgcc actcgtcgtg aatggctgga ggcaacaggc
    57181 gcttacgatc gtcgctggaa tatgctgttg agtctgcgct cctgggcgct ggttggcagt
    57241 agcgtgatgg ctatctggac gattcgccat cctaatatgc tggtccgctg ggccagacgc
    57301 ggttttggtg tatggagcgc ctggcgtctg gtgaaaacaa ccctcaagca acaacagctt
    57361 cgcggttaac ttcctctctg gccggagaaa ttccggcctt attcctcaaa ttttttgaaa
    57421 atatttgaca gttttccttg ctaacaatca cgcttcgcca cgtttatgat tctctccatc
    57481 gacagcaacg acgctaatag cgcgcccttg cacaaaaaaa caatcagcag cctgagcggc
    57541 gcagtggaga gtatgatgaa aaaattagaa gatgttggtg tactggtagc gcgtatttta
    57601 atgccgattc tgtttattac cgcaggctgg ggaaaaatta ctggctacgc gggtacccaa
    57661 caatatatgg aagcaatggg cgtccccggc tttatgctgc cgctggtgat tctgcttgag
    57721 tttggtggtg gtctggcaat cctgttcggt ttcctgacac gtaccacagc cctgtttact
    57781 gcgggcttta cgctgctgac ggcattttta tttcacagca attttgcaga aggcatgaac
    57841 tcgctgatgt ttatgaaaaa cctgactatt tctggcggat tcctgctgct ggcaattacc
    57901 ggtccaggcg cattcagcat cgaccgcctg ctgaataaaa agtggtaagc acgcgctata
    57961 cttaacgata aaaaacgagg gggaagctcc tcgtttttgc tattggagga tagaaaaatg
    58021 ggtcaactga ttgacggcgt ctggcatgac acctggtacg ataccaaatc caccggcggt
    58081 aaatttcaac gttcagcttc cgcatttcgt aactggctca ctgccgatgg cgcgccaggc
    58141 cccactggca aaggcggttt tgccgcagag aaagatcgtt atcatctcta tgtttcactc
    58201 gcctgcccgt gggcacaccg cacgctgatc atgcgtaagc tcaaaggact ggaaccgttt
    58261 atttccgttt ccgtagtgaa cccgctgatg ctggaaaacg gctggacctt tgatgacagt
    58321 tttccgggag caagcggcga cacgctctat caacatgaat ttctgtatca gctttatctc
    58381 cacgccgatc cacactacag cggacgagtt actgttcccg tgctgtggga caaaaaaaac
    58441 cacaccatcg tcagcaacga atcagcagaa atcatacgca tgttcaatac cgcgtttgat
    58501 gcgctgggtg cgaaagctgg agattactac ccaccagccc tgcaaccgaa aattgacgaa
    58561 cttaacggct ggatttacga caccattaac aatggcgtgt ataaagccgg ttttgccacc
    58621 agccagcaag cctacgacga ggcggtggcg aaagtgtttg aatcgctggc gcgactggaa
    58681 cagattttag gtcagcaccg ttacctgacc ggcaaccagc taaccgaagc cgatattcgc
    58741 ctgtggacca cgctggtgcg ttttgatcca gtgtatgtga cccacttcaa gtgtgataag
    58801 caccgcatca gcgattacct gaatctgtat ggcttcctgc gcgatatcta ccagatgccg
    58861 ggaattgccg aaacagtcaa tttcgatcac atccgtaatc attacttccg cagccataag
    58921 accatcaacc ctacggggat tatttcaatt ggtccgtggc aggatctcga tgaaccgcat
    58981 ggacgagatg ttcgcttcgg ttaaaaaaag gcaccttacg aggtgcctct gataattagc
    59041 taagtacgtg ttgttcatgt cggatgcggc gtaaacgcct tatcaaacct acaaaacatt
    59101 tatattcaat atgttgcaat gaccgcgcag gccagataag cgtagcgcat ctggcaattt
    59161 tgcgtttgcc cttatccttc acagcgcctt attaattcat cgcgataata tttactgcca
    59221 tttcattcac gtctattctt aatttgctgc tttaaaaaca agtgattgag caaaattgag
    59281 gcaaaaatgg actggtatct gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg
    59341 cggcgcaaag agtactggat gtttattctg gtcaacatca tctttacgtt cgtgctgggg
    59401 ctactggata aaatgttagg ctggcaacgt gccggtggcg aaggcatcct gacgacaatc
    59461 tacggtattc tggtgttttt accatggtgg gcagttcagt tccgccgcct gcacgacacc
    59521 gaccgctcgg cgtggtgggc actgctattc ttaatcccgt ttatcggctg gcttattatc
    59581 atcgtcttta actgccaggc gggtacgcca ggcgaaaacc gctttggacc agatccgaaa
    59641 ctggaacaag aataataatt aagcaccttg taaaaggtgc ttaatcgatt attttccgct
    59701 aaaaagtttg ggaatttccc gcagacacca ggatttcgcc tcccccatac tgtcacgacg
    59761 ccaggccata ataatatcga tctcgctggt cgattccggg ctgacgacac gcaaccgccc
    59821 ttccgcaata tctttttcga ccatcggata cggcatcgtc gccacgccaa gccccgccag
    59881 taatgcctga cgtttatctt caatcgtgct caccgttaag cgcggctgtt tgtccagcaa
    59941 ctgtacggtc aacaccgggc gctcacgagc ggtatccgcc accgcaattc cacgatattt
    60001 cacgcgcgtc acttcagata acggttccgg ctcctgatga atcgggtgat ccggcgcggc
    60061 aacatagacg ttcattaagg tatagagttt gcgcgagttg atctccgacg aggaacgaaa
    60121 atgcatatcc ggcgcgataa cgatatccgc ccgcccctgc tccagccgtt cccacgcccc
    60181 cgccagcact tcggtgataa ttgccagttg ggtattggct tttgccgcca gtttgtcgat
    60241 taacgggaaa aaggcaggtg tcggtaccag cgcctcggtc acaatggtga gatgcgtttc
    60301 ccaaccgcgc gcgagggctt ccgcatcggt agtcagttta tctgcggctt ccagcaaaac
    60361 gcgccctcgc tccagcaaca tcctcccgac attggtgaat ttggtgcgat ggcccgagcg
    60421 gtcaaacagc accacatcca gctcttcttc cagtttttgc atggtgtagc taagtgcgga
    60481 aggcacgcgt cccagctcat ccgccgccgc cgcaaaactg ccccggcgat cgatcgcatc
    60541 cataacccgt agtgcttcca gcgttaatgc cctttctttg gccatttcgt tctcattcag
    60601 gaaatttgaa cataccgggc agaatatctg gctaacaatg tagcgtccag ccccttacca
    60661 taaaaggaag taaagagagg tcaagaatta tgattactac ccgaactgcc aggcagtgtg
    60721 gacaagcaga ctacggatgg ttgcaggccc ggtatacttt ttcctttgga cactacttcg
    60781 acccgaaatt gttaggctat gcctctctgc gcgtgcttaa ccaggaagtg ctggccccag
    60841 gtgccgcctt tcagccacga acctatccca aagtcgatat tttaaatgtg attctggatg
    60901 gagaagctga gtatcgcgac agcgaaggca atcatgttca ggccagcact ggtgaggtgt
    60961 tgctgctctc tacccagccg ggtgtcagct atagcgaaca caatctcagc aaagacaaac
    61021 cgttaacgcg aatgcagctt tggctggatg cctgcccgca gcgagagaat ccgctgattc
    61081 aaaagctggc gcttaatatg agcaagcagc aattaatcgc ctcgccagaa ggtgggatgg
    61141 gaagcctgca attgcgccag caagtgtggc tgcaccatat cgtgctcgac aaaggcgaaa
    61201 gtgcgaattt ccagttgcat gggccacgcg cgtatttgca atcgattcac gggaaatttc
    61261 atgcgcttac gcatcatgaa gagaaagcgg cgctaacctg cggtgatggg gcgtttattc
    61321 gtgacgaggc taacattacg ctggttgccg attccccact gcgcgctttg ctgatagatt
    61381 tgcctgtcta gttgttttta cagggagatg atgatgagta aaaaatcggc caaaaagcgc
    61441 cagccagtga agcccgtggt ggcgaaagaa cctgctcgta ccgccaaaaa ttttggctat
    61501 gaagagatgt tgagcgaact ggaagccatc gtcgcggatg ctgaaacgcg tttagccgag
    61561 gatgaagcta ccgcgtaaag acaggttgcc ggatgcggcg caaacgcctt atccggcaca
    61621 atacccaaaa ccgggcgttt atctggcctt gctcgccata atctcgataa tctgccgatc
    61681 cgtttgctgc atcgaatggc ttgctaacgc acacaggttg gcaatcgact gctcaacatc
    61741 atgcgccaca atcccttcat tgccggtcac ggcggtatca tccagcgcca ttaacaccgc
    61801 tttccacgca gccgaagcac tggtcgaaac cttcatcgcg cagctgttcg atgcaccatc
    61861 gcaaatcatg ccgctgacat cgccgatcat actgctgatc gccatcgaga tggtttcata
    61921 acgcccatcc accagccatg ccatcccggc agcggccccc attgctgcgg tcgttgcggc
    61981 acacagcgca gacaaacgcg gtaactggtt atggatgtaa atcgcgctca aatgcgaaag
    62041 catcagcgca cgcgccagcc gttcatcatc cgctccgaag tgttcagcta ccaccaccac
    62101 gggcattgtt gcggtgatcc cctggttacc cgagccggag ttactcattg ccggaagcgt
    62161 agcgccgccc atacgcgcat cggatgccgc gctggtacga atcacaatgg atgaagagag
    62221 atctttcgcc agcaaaccgc gcgcgcactg tttttccagc gtcgcaccaa tatgcagccc
    62281 ccagttaccg ctcaaacctt cctgcgataa cgcacaattt aacttcgcag aatcgagaat
    62341 aaagcggatc gccgcaaacg ggacttcatt gacgaacttc aggatctcag ccagcgtcgt
    62401 cctggaaagc accgtcagcg gcgactcttg ctcgccctct gccacacacg cctgctgggt
    62461 aaacaccaca ccattgtgcg tctcaatatg cacaatgttg gtatgcccgc cgacgatggt
    62521 gacacacgcc catttctcac cgttccagac tttggcgcgt gagaagagga tttcatcgca
    62581 aggttcctgg atcttaactg agactttccc cgccgccagc agtgctttgg catcggaaat
    62641 tgcctgcgca gttgcgtctt tcagcacttc cagcccggcg ttagcatttc cacctaacgc
    62701 tcccagcgcc gccgcaattg gcagccccac cattcctgta ccgggaacgg tgacacccag
    62761 accgttcttc atcagatttg gcgaaaccca ggcttctaca cgttcaaccg gaccttccag
    62821 ttcagccgca gcaaccgccg ccgccagcgc cagtgaaatc ggttcagtac accccagcgc
    62881 cggttttact tcctcctgaa cggcgaggat gtaacgctgc cataacggat ttaaagtcga
    62941 atcaaacata ttaaaaacct taaaatttca ggtaaatcaa gaaaatgcca ggaacggaga
    63001 aacacaaagc aacaaaccag tgacgataat caggtacaaa gacatccctt tgtatttgtg
    63061 caatgccggt actttgtaaa ccagccacgc cgggatcagg caccctacca tgccgaaaat
    63121 cgggctacag atagaggtga agctcaacac cggggcgttc agtacgatgg cgctccaggc
    63181 cagcaaaatg gcgaaaatca tgatgccgcg ctgaacgaga ttttcgttaa tcttctcggc
    63241 aggtatcttg cgacgcagga tgttcattac gatcccttgc gttgcttcgc gaaagcctaa
    63301 atagacgcca aagaatgcag tcattacagc aaagatattg agaatgacgc tgaccacttt
    63361 cacccacgct gcgccgtcac cgctaataaa ctgtgcggca atcgccagcg cggaaatatt
    63421 ctgctcgtag gcttttaccg cttcgtcatg tcccatcgcc agcgtgaacg acacggcgta
    63481 gaaaaagacg gtgacaaaca aaatgccaaa cgcgatattc attgcccgca atgctttatg
    63541 ccgcgccact tcaattgatt tttcccgcga gcgataagag atcaccatcg ggcttaacgt
    63601 ctggataaat agaatcgacg tcagggtaaa cggcagcgta ataatggcgt ttttcatcag
    63661 cagccccagc ggcggtagcg aaccgacgtt atacagatgc cacattccca ccatcgacac
    63721 gcccagcgcc gcgaccacca gcagcttggt cagcaccatg ccggtcgaaa ttttgaataa
    63781 caatttctcg ccgcgtgagg agatcgccac cagaatgcaa atcagtacca gaccataaaa
    63841 gggactgtct gacagcaacc cttccgtcac gccgaaggta tgcaggtagg aagcactatc
    63901 gttggtgatg gcggtggaat agacgaacat ccaaatcacc agcattacga aatagagtgc
    63961 acctaacagg atgccccagt ttttacctaa ataaccgctg atgacgctcg ggtaatcttt
    64021 acattctggt gattctgcca gcgtattaat aaacaaccgc tgaaacagat acattgccgg
    64081 gtaaccaatc accgatgaga gcaaaaatac ccacaatccc atcaaaccga cctgcaccgg
    64141 gagaaaaaca atccccgcgc caatcgccat cccgatactc ataatcaccc agccagtgtc
    64201 agtactgtcg aatttgatcg cttctcgcca ctcgctctca ctcattccag cacgacccgc
    64261 cggggtcgaa gcgtctgcaa tgacgccttt attcgatgca atttccataa tttctcgctc
    64321 aatattttgt agggcttatt atttttttcc gagccgcatc aaggcgatat gcggtctacg
    64381 tttttgcagg cgagttatta gaataaagaa atgatacgcg tgagatcaga gaaaatcttc
    64441 acaatcaaga cttttaaata gcaattgtgg
//
LOCUS       DACADF010000007        58501 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.007, whole genome shotgun sequence.
ACCESSION   DACADF010000007 DACADF010000000
VERSION     DACADF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.007"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(72..287)
                     /locus_tag="ISB30_000454"
     CDS             complement(72..287)
                     /locus_tag="ISB30_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988133.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVIA"
     gene            complement(350..1822)
                     /gene="der"
                     /locus_tag="ISB30_000455"
     CDS             complement(350..1822)
                     /gene="der"
                     /locus_tag="ISB30_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP1988134.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1940..3118)
                     /gene="bamB"
                     /locus_tag="ISB30_000456"
     CDS             complement(1940..3118)
                     /gene="bamB"
                     /locus_tag="ISB30_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAP1988135.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTVWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3129..3749)
                     /locus_tag="ISB30_000457"
     CDS             complement(3129..3749)
                     /locus_tag="ISB30_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAP1988136.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3767..5041)
                     /gene="hisS"
                     /locus_tag="ISB30_000458"
     CDS             complement(3767..5041)
                     /gene="hisS"
                     /locus_tag="ISB30_000458"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP1988137.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5152..6270)
                     /gene="ispG"
                     /locus_tag="ISB30_000459"
                     /gene_synonym="gcpE"
     CDS             complement(5152..6270)
                     /gene="ispG"
                     /locus_tag="ISB30_000459"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAP1988138.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6297..7304)
                     /gene="rodZ"
                     /locus_tag="ISB30_000460"
     CDS             complement(6297..7304)
                     /gene="rodZ"
                     /locus_tag="ISB30_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAP1988139.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTSDCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            complement(7589..8743)
                     /gene="trmG/rlmN"
                     /locus_tag="ISB30_000461"
     CDS             complement(7589..8743)
                     /gene="trmG/rlmN"
                     /locus_tag="ISB30_000461"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAP1988140.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8893..9324)
                     /gene="ndk"
                     /locus_tag="ISB30_000462"
     CDS             complement(8893..9324)
                     /gene="ndk"
                     /locus_tag="ISB30_000462"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP1988141.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9473..11785)
                     /gene="pbpC"
                     /locus_tag="ISB30_000463"
     CDS             complement(9473..11785)
                     /gene="pbpC"
                     /locus_tag="ISB30_000463"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HAP1988142.1"
                     /translation="MPRLLTKRGCWIMLTAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFAYGLALDEGVIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11786..16747)
                     /gene="yfhM"
                     /locus_tag="ISB30_000464"
     CDS             complement(11786..16747)
                     /gene="yfhM"
                     /locus_tag="ISB30_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HAP1988143.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDDEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAALQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKNGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGALANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALTGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMISIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKIMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGGASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            16954..17799
                     /gene="sseA"
                     /locus_tag="ISB30_000465"
     CDS             16954..17799
                     /gene="sseA"
                     /locus_tag="ISB30_000465"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAP1988144.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWLRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(18292..19068)
                     /gene="sseB"
                     /locus_tag="ISB30_000466"
     CDS             complement(18292..19068)
                     /gene="sseB"
                     /locus_tag="ISB30_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAP1988145.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19210..20493)
                     /gene="pepB"
                     /locus_tag="ISB30_000467"
     CDS             complement(19210..20493)
                     /gene="pepB"
                     /locus_tag="ISB30_000467"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAP1988146.1"
                     /translation="MTEAMKITLSTQPADVRWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKVDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPELDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLICNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20552..20752)
                     /gene="iscX"
                     /locus_tag="ISB30_000468"
     CDS             complement(20552..20752)
                     /gene="iscX"
                     /locus_tag="ISB30_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAP1988147.1"
                     /translation="MGLKWTDSREIGEALYDAYPDIDPKTVRFTDMHQWICALEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20764..21099)
                     /gene="fdx"
                     /locus_tag="ISB30_000469"
     CDS             complement(20764..21099)
                     /gene="fdx"
                     /locus_tag="ISB30_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAP1988148.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21101..22951)
                     /gene="hscA"
                     /locus_tag="ISB30_000470"
     CDS             complement(21101..22951)
                     /gene="hscA"
                     /locus_tag="ISB30_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAP1988149.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(22968..23483)
                     /gene="hscB"
                     /locus_tag="ISB30_000471"
     CDS             complement(22968..23483)
                     /gene="hscB"
                     /locus_tag="ISB30_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAP1988150.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23579..23902)
                     /gene="iscA"
                     /locus_tag="ISB30_000472"
     CDS             complement(23579..23902)
                     /gene="iscA"
                     /locus_tag="ISB30_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAP1988151.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(23919..24305)
                     /gene="iscU"
                     /locus_tag="ISB30_000473"
     CDS             complement(23919..24305)
                     /gene="iscU"
                     /locus_tag="ISB30_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAP1988152.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24333..25547)
                     /gene="iscS"
                     /locus_tag="ISB30_000474"
     CDS             complement(24333..25547)
                     /gene="iscS"
                     /locus_tag="ISB30_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP1988153.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25659..26147)
                     /gene="iscR"
                     /locus_tag="ISB30_000475"
     CDS             complement(25659..26147)
                     /gene="iscR"
                     /locus_tag="ISB30_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAP1988154.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26418..27158)
                     /gene="trmJ"
                     /locus_tag="ISB30_000476"
     CDS             complement(26418..27158)
                     /gene="trmJ"
                     /locus_tag="ISB30_000476"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAP1988155.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27277..28080
                     /gene="suhB"
                     /locus_tag="ISB30_000477"
     CDS             27277..28080
                     /gene="suhB"
                     /locus_tag="ISB30_000477"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAP1988156.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDSTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28225..29079
                     /locus_tag="ISB30_000478"
     CDS             28225..29079
                     /locus_tag="ISB30_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1988157.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSLAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29270..30550
                     /gene="csiE"
                     /locus_tag="ISB30_000479"
     CDS             29270..30550
                     /gene="csiE"
                     /locus_tag="ISB30_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAP1988158.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSRFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30542..31681)
                     /locus_tag="ISB30_000480"
     CDS             complement(30542..31681)
                     /locus_tag="ISB30_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAP1988159.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            32051..32473
                     /locus_tag="ISB30_000481"
     CDS             32051..32473
                     /locus_tag="ISB30_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAP1988160.1"
                     /translation="MNSLRYFDFGTARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(32521..33393)
                     /locus_tag="ISB30_000482"
     CDS             complement(32521..33393)
                     /locus_tag="ISB30_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAP1988161.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSRSDDSLC
                     LVYEHLSGVYHYRVSQAFHLTADTLTVTLAVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGKARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(33405..34499)
                     /locus_tag="ISB30_000483"
     CDS             complement(33405..34499)
                     /locus_tag="ISB30_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP1988162.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(34532..35530)
                     /locus_tag="ISB30_000484"
     CDS             complement(34532..35530)
                     /locus_tag="ISB30_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1988163.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFSVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(35533..37062)
                     /locus_tag="ISB30_000485"
     CDS             complement(35533..37062)
                     /locus_tag="ISB30_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP1988164.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLKKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSTDEFYRG
                     T"
     gene            complement(37085..38068)
                     /locus_tag="ISB30_000486"
     CDS             complement(37085..38068)
                     /locus_tag="ISB30_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP1988165.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKTRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(38165..41446)
                     /locus_tag="ISB30_000487"
     CDS             complement(38165..41446)
                     /locus_tag="ISB30_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAP1988166.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHDDGAQTVWVGETESMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYHEHQPQALPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVVYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPAL
                     AADMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDAFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLEDNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDDREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAVCANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            41564..42757
                     /locus_tag="ISB30_000488"
     CDS             41564..42757
                     /locus_tag="ISB30_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP1988167.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPIEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQVRDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLNPEKLTTAQLIAAWQSGEPWFTSWVDHSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(42821..44074)
                     /locus_tag="ISB30_000489"
     CDS             complement(42821..44074)
                     /locus_tag="ISB30_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP1988168.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            44403..45593
                     /gene="hmpA"
                     /locus_tag="ISB30_000490"
     CDS             44403..45593
                     /gene="hmpA"
                     /locus_tag="ISB30_000490"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAP1988169.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAVAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(45638..45976)
                     /gene="glnB"
                     /locus_tag="ISB30_000491"
     CDS             complement(45638..45976)
                     /gene="glnB"
                     /locus_tag="ISB30_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAP1988170.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(46037..47371)
                     /gene="glrR"
                     /locus_tag="ISB30_000492"
     CDS             complement(46037..47371)
                     /gene="glrR"
                     /locus_tag="ISB30_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAP1988171.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRNELDANDFKE"
     gene            complement(47361..48074)
                     /gene="qseG"
                     /locus_tag="ISB30_000493"
     CDS             complement(47361..48074)
                     /gene="qseG"
                     /locus_tag="ISB30_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAP1988172.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNRPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            complement(48239..49666)
                     /gene="qseE"
                     /locus_tag="ISB30_000494"
     CDS             complement(48239..49666)
                     /gene="qseE"
                     /locus_tag="ISB30_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAP1988173.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETL
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(50242..54129)
                     /gene="purL"
                     /locus_tag="ISB30_000495"
                     /gene_synonym="purI"
     CDS             complement(50242..54129)
                     /gene="purL"
                     /locus_tag="ISB30_000495"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAP1988174.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGMAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKDEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGVRPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            54387..55943
                     /gene="mltF"
                     /locus_tag="ISB30_000496"
     CDS             54387..55943
                     /gene="mltF"
                     /locus_tag="ISB30_000496"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAP1988175.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(55940..56476)
                     /gene="tadA"
                     /locus_tag="ISB30_000497"
     CDS             complement(55940..56476)
                     /gene="tadA"
                     /locus_tag="ISB30_000497"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP1988176.1"
                     /translation="MRRAFITGVFFLSEVEFSYEYWMRHAMTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(56501..57136)
                     /gene="yfhb"
                     /locus_tag="ISB30_000498"
     CDS             complement(56501..57136)
                     /gene="yfhb"
                     /locus_tag="ISB30_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAP1988177.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            57345..58193
                     /locus_tag="ISB30_000499"
     CDS             57345..58193
                     /locus_tag="ISB30_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP1988178.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            58249..>58501
                     /gene="yfhL"
                     /locus_tag="ISB30_000500"
     CDS             58249..>58501
                     /gene="yfhL"
                     /locus_tag="ISB30_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAP1988179.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGEHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHAD"
BASE COUNT        13838 a        16306 c        14567 g        13790 t
ORIGIN      
        1 ttccaccata acagattgtt ttatcatttg cacattaaat acaaatgaca aaacaataca
       61 ttcaagtgat attaagcaat aacaaattca acgcgttttt tagatattaa accgtgccca
      121 tgttggcaga aataatccac agcaccacaa gcttttaaca cttgtaacgg ctgatgacac
      181 tcagggcaaa gtgcctggac ggtaaagtct ttgtcgcaag ttgcacagtg agcagtctcg
      241 ccattgcgtt cgagaggggt tttgcaggtt ggacagttga gtgccataaa tactcctcgc
      301 gtgttattcg gtttccctct ccgtgtggaa agggaaacca gataagtgat tacttacttt
      361 tcttgatgtg cttcatcaga cgcttacgct tgcgcatctg agtcggcgtc agggtattac
      421 gcttgttcgc atacgggttt tccccttctt tgaactgaat acgaatcggc gatcccatta
      481 cgtccaggga tttgcggaag tagttcatca ggtaacgctt gtaggaatca ggcaggtctt
      541 tcacctgatt gccgtgaatc accacaatcg gcgggttata acccccggcg tgggcgtatt
      601 tcagcttcac acgacgaccg cgtaccagcg gcggttggtg atcttcaaca gccatcgtca
      661 tgatgcgcgt cagcatagag gtccccacac gacgggtgga gctgtcatac gcttcacgta
      721 ctgattcaaa caagtttcca acaccactgc cgtgcaaggc agagataaag tgcacacgag
      781 caaaatcgat aaagcccaga cggaagtcca gcgtctcttt cacctgctct ttcacttcct
      841 ggctcaggcc gtcccacttg ttcactacaa tcacaagtga gcgcccacta ttgagaataa
      901 agcccagcaa ggagagatcc tgatcggaaa taccttcgcg cgcatcaatc accaacatca
      961 ccacgttggc atcttcaatg gcctgcaacg ttttgattac cgagaatttc tctaccgcat
     1021 cggtgatttt gccgcgttta cgtacgccag cggtgtcaat gagcacatac tcacgtccgt
     1081 cgcgttccat tggaatgtag atactgtcac gcgtcgtgcc aggcatgtcg taaacaacaa
     1141 cgcgctcttc accaagaata cggttagtga gtgtagactt acctacgttc gggcgaccca
     1201 caatcgccag tttgatcggc agactttgcg ggtcgaagtc gtcttcctct tcttcttcgc
     1261 cgttctcttc cgcttcaaat tgcgcccagt attcggcgtc ttcgtcgact tcttcttgcg
     1321 gtgcgagatc ttccatccac ggcagcagca cgtgctccag cagacttaat acgccacgac
     1381 cgtgagacgc ggcgatcggg tagatttcac ctaaaccaag cgcgtagaaa tcaaccactg
     1441 cctgatcggg atccagaccg tcagttttgt ttgccaccag gaaggtcggt ttttcacggg
     1501 agcgcagatg tttagcaatc gcttcatccg ccggcatcag gcccgcgcgc gcatccacca
     1561 taaacagtac gacgtccgct tcttcaatcg ccagcagcga ctgttccgcc atgcgggttt
     1621 ctacaccgtc ttctgtgcca tcaatcccgc cggtatcaat gcagataaac tcacggcctt
     1681 caatttccgc acgaccgtac ttacggtcac gagtcagacc cgggaaatcc gcaaccagcg
     1741 catctcgggt gcgagttaga cggttaaata acgtggattt tcctacgtta gggcgcccga
     1801 caagcgcgac cacaggtacc atgtttaaag cctcattttt ataaatcatc agacaacgca
     1861 cgctatattc gtgtcgttgt taaaaacagg aaaacggccc ctgtccagga gccgttttca
     1921 aagtgaacga cagagacgat taacgtgtaa tagagtacac ggttccgtct tttgcctgga
     1981 tcagcagttt gccgtcagca gcaaccggtt cagtctggaa accggaacta tcaacttttt
     2041 gctgggcaac gaaacgacca tcttcgacgt taatccagtg cagataacct tcactgtcgc
     2101 ctaccaccag gttgccatta tacagcaccg gagaagtcag caggcgatgc agcagatcgc
     2161 tttgtgtcca cagcgtaacg ccgccatcaa tggtcaacgc catcacccgg tcattttgat
     2221 cgaccagata gatgcgattg ccgtcgacga tgaaatcatt caccgaaccc agttcgcgtt
     2281 tccacataat ctgaccactg cgcagatcaa gcgccgtcag gttaccatta taggccagcg
     2341 cgaaaacaac gccgttaaca acgacgggag tcgtgtcaac atcgctcaga cggtcaattt
     2401 cggtagaacc ggtcgcctgg gaaatacgct gctgccaaat catctggccc tgttccatca
     2461 gcactgcgct gacgcgacca ttatcgccac ccacgacggc cgcaccaaaa gccgttgccg
     2521 gcgcagactc gccacgcaaa gagagcgaag gcatatcgag gttaactgtc catttgacag
     2581 cgccgtcagc ttcgttcagc gcttgtaact gaccgttact ggtgtggatt aacaccagac
     2641 cgtcgctgac caccgggcgc gaaagtgctt cacccgcgac tttagtttgc catgccacag
     2701 taccatcgct ggtattcagc gcgtaaacct gcgccttttc ggtgccaatg tagacatgcc
     2761 caccagacac ggtcacgccg ccagaaagta atgcaggatc tttagagaac cagccatctt
     2821 tctcggccag gttgacagac cagatttctt tgccgtcatc cgcattcagc gcttttacta
     2881 aaccagcgcg gtccgctgca tagacaacgt tgtccgccag tgccggatga agattggaat
     2941 agaagttgcc aatgccgcta ccaacggaag tgctccacac agtagtcggc gtaaactggt
     3001 tttcaacggt tggcaatggg gacatcttta ccacatcttc ttcgctgtta aacagcgaac
     3061 agccgcttaa aagggtaacg gaaagcagtc ctggcagcag taatttacgc aattgcatcg
     3121 ggtccctctc agatggacaa attattaatt ttcatctgca tcatttcgct cagtgccggg
     3181 gtaacatcgc ttttcacgcc tgcttcccat gcactacgcg caccttgctt atcacctttg
     3241 ctcagcaatg cttcaccacg caggtcggca acaatggcag cccacccttc acctttgata
     3301 gcatcaaggg tttttagcgc ggcatcagcc tgcttgagct gtacctgaac gcgagcaaga
     3361 cgcagattta tcacggcttt gagattttca tcgctcgtgt ctgccagccc ctgttgtaac
     3421 tgggcggcag ctttctctag ttcatttttg tcaacaaatt gctgcgcaag ttccaaagaa
     3481 gccagcgcac cataagtatt tttattttca gcagcaaatt tttccgccgc tgggatgcta
     3541 tccggtttgc cttcgctcac tgcggtaacc gcattttgat aggcaagaga agcggagcgt
     3601 gcagaatcaa cctgatggct attccagtag cgccagccaa tcagtgcgcc aacgcccaaa
     3661 atcaccccaa cagccagtgc tttgccattt tcagcaaaaa agcgtttaac cgcttcaacc
     3721 tggtcgtttt cgttctcgta aatttccacg ctgtccttct ccttccttaa cccagtaacg
     3781 tgcgcaaatg cgcggctacg ctatcctgcg caactgcagt ttgctcacca gagcgcaaat
     3841 ccttcactac tgctgtgccg ttagccactt cagactcacc cagcaccaca gcaacgcggg
     3901 caccccattt atcagcacgg gcaaactgtt tcttaaagtt gccgccgccg tggttggtca
     3961 tcagtttcac acccggtaat tcatcacgca gacgctctgc taatgccata gccgcagatt
     4021 gtgtgtcagc acctgaagcc accaggtata tatcgacaac aggatcggct ttaaattccg
     4081 gattaacggc ctgaactaac aatacaagac gttcgaggcc catcgcaaaa ccgactgccg
     4141 gtgttgcacg accacccagt tgttccacca gaccgtcata acgaccacct gcacacacgg
     4201 tgccctggga accgaggctg ttagtcaccc actcgaaaac ggtacggtta tagtaatcca
     4261 gaccgcgcac cagacgctgg tttacagtgt aagcgatccc cgcgctctca agcagtttgc
     4321 acagaccggc aaagtgctca cgagattcct cgtccagata atcgcctaat gccggagcgt
     4381 cgttgagaag cacctgcact tccggatttt ttgaatccag tacgcgcaac gggttagtgt
     4441 acatgcggcg tttgcagtct tcgtccagct tttctttatg ctgctcaagg aatgccacca
     4501 gcgcatcgcg gtaattcgca cgagcttcca gcgaaccgat agagttcagc tcaagagtta
     4561 cgtgctcgga aatacccagc gcgcgccacc agcgggcagt gagcataatc agttcggcgt
     4621 cgatatccgg accttgcaga ccgaaaactt cgcagcccaa ctgatggaac tgacgataac
     4681 gccctttctg cggacgctcg tgacggaaca taggcccgat ataccacaga cgctgttcct
     4741 gattgtacag aagaccatgc tcgatgccgg cgcgtacaca gcccgccgtc ccttcagggc
     4801 gcagagtcag gctgtcgcca ttgcgatcct caaaggtgta catctctttt tcaaccacgt
     4861 cggtgacttc accaatcgca cgtttgaata gcggggtctg ctctacaatc ggcaagcgga
     4921 tttcactgta accgtagctg ccgagcacgt ttttcagtgt gccttcaatg cgctgccaga
     4981 tggccgtttc gccaggcagg taatcgttca tgccgcgaat ggcttgaatg ttttttgcca
     5041 cgtttattct ctttctgaat ataaaaatga accctcaacg cttccctcaa tgtttcggga
     5101 gccatgcggg ttcaatcata cacgggaagc gaggcgcttc ccatcacgtt attatttttc
     5161 aacctgctga acgtcaattc gacgcgcttc gtccagctga ctggctttcg cacgaatgcg
     5221 cgcttccagc tgatcgatca tatcgttgtt gtccagacgg tctttgcgca cgccatcttc
     5281 atagaggccg cttttcttgt tgccgccagt gacgccgagt gtagaaacca gcgcctcacc
     5341 tgggccattc accacgcagc cgataatcga aacgtccatc ggagtgatta tatcttccag
     5401 gcgttgctcc agcgcgttaa ccgtaccgat aacatcaaat tcctgacgcg aacaggtcgg
     5461 gcaggcgatg aagttgatcc cgcgcgaacg gatacgcagc gatttcaaaa tatcgaaacc
     5521 gactttgatc tcttcgaccg gatcggccgc cagcgatacg cgcagcgtgt cgccgatgcc
     5581 ttcagacagc agcagaccta aaccaatagc ggattttact gccccgctgc gcgcaccacc
     5641 ggcttcggtg atccccagat gcagcggctg atcgatttgt tttgccagca aacgataaga
     5701 ctcaacagcg aggaagacgt cagacgcttt cacactgact ttgaactgat cgaagttcag
     5761 gcgatcgaga tgatcaacat gacgcatggc ggattccagc aacgcctgcg gcgtcggttc
     5821 gccatacttt tcttgcagat ctttttccaa cgatccggcg ttaacgccaa tacggatcgg
     5881 aatgttttta tcgcgcgcac agtcaaccac catgcgaata cgctcttcat taccgatatt
     5941 gccagggtta atacgcagac aatcgacgcc gtattccgct actttcagcg caatgcgata
     6001 gtcgaagtgg atgtcagcca ccagcggcac gttaacctgc tgtttgatga gtttgaacgc
     6061 ttctgccgcg tccatcgtcg gtacggagac acggacgata tcagcgccaa cgcgttccag
     6121 cgctttgatt tgattgaccg ttgcttcgac gtctgtcgta cgcgtattgg tcatggactg
     6181 tacggcgatg ggagcaccat cgccaatcgg cacattccca acgtaaatac gtgttgattt
     6241 tctacgttga attggagcct ggttatgcat gaaaaatctc ccgcgttacc cgtctgttac
     6301 tgcgccggtg attgttcggc attgagggtc agacgcgcaa cctggttagt tctgataaaa
     6361 cgactcagat cgacaggttt cccttgatac tggatctgta ctgcggctgg cgcaccaatt
     6421 ttcagtttgt acggtgcctg gccggttaag ttcaaattac cgtctttacg ctgcataccg
     6481 ctaaacagtt ttttaccggt agcatcggtg acttccagcc agcaatcgga agtaaagttc
     6541 atcaccagcg cattcgggtc aaccgccggc gtggtcacgc cagcctgatc ggttggcaac
     6601 ggcgcagcgc catctggtgt tgttgttgcc gctggtgccg gagtcgcagc ggtatcaaca
     6661 tttgcctgcg aaggtggaac aaccgcattc tgttgcggat caacagctgg tgctggcgca
     6721 gttacggcag gtgtttgcgt gttggttgcg gtggtatcca caggtgatgt cggcgtggtc
     6781 gccatagcct gatctgtggt tgtcgacgta tctaacggta cactctggct ttgattatta
     6841 ttcagttccg cagaagattg atcggccata gtggtgatct cttcctgctg agctttgtgg
     6901 tcttgccacc accaggcacc gctcagacca ataaccacaa acaacaccag ccaggtgaag
     6961 gtcatcagcc agccgtcacg ttttttgcgg cgtttaccga gggaaaaact ctgcatcggc
     7021 gcaacttttg cagcccgtag tggagcctgc ttttccagcc ctggcagcag ttcttcttct
     7081 ggaatatgta ccagacgcgc ataagagcgg atatatccgc gcaggaatgt tgaagcaaga
     7141 tcggcgggtg ccttatcttc ttcaatgtcg cgtaccgtgg aaaccttcag gcaaagtcgc
     7201 tcggcaacgg cctgttgact aagtcctagt tgttcgcgag cattacgcag gcgagcgccg
     7261 gtagtaagtg cttcattttg gtcgtgcgtg gcttcagtat tcattcgctg caggtacgtt
     7321 taaatgagaa ttgagatgcc ggtgaaccat catgctcacc cacaccgcga aacatccgtt
     7381 aagttaaccg ttatcataca gtataagacc gtctgtgtgc tcgtgacaaa gcccacattc
     7441 gctaaggcta acttactgtt gcatcgttac atactgcctt aaagtcagca aaaacgcacc
     7501 gttaatattg accagacaat tgcaacttaa tacttcattc ataaaagatt acgccacggt
     7561 acataaagta accgtggcgt aatggctatc agaccgcttt aatgtcgatg gcttcaccct
     7621 gcatccgttt acgcagggta cgtttcgtac ggtcgataac atcgcccgcc aactgaccac
     7681 aggcagcatc aatgtcatca ccacgagttt tacgcacaat agtggtgaaa ccgtagctca
     7741 tcagcacttt tgagaaacgg tcgatacggc tgttcgaact gcgtccatac ggcgcgcccg
     7801 ggaacgggtt ccacgggatc aggttgatct tacacggcgt atctttcagc agttctgcca
     7861 gttggtgcgc gtgttcagtg ccgtcgttaa cgtggtcaag catcacgtat tcaatagtga
     7921 ctcggccctg attggcgttg gatttctcca gataacggcg caccgccgca aggaacgttt
     7981 cgatattgta ctttttgttg atcggcacaa tttcgtcacg aatttcgtcg ttcggcgcgt
     8041 gcagggaaat tgccagcgca acgtcgatca tatcgccgag tttatccagc gccggaacca
     8101 caccggaagt ggaaagcgtg acgcgacgtt ttgacaggcc aaaaccgaaa tcatcaagca
     8161 tgatttccat cgccggaacg acgttgttca ggttgagcag cggctcgccc atacccatca
     8221 tcactacgtt agtgattggg cgctgaccgg tgacttttgc tgcgccgaca attttcgccg
     8281 cacgccacac ctggccgata atttccgata cccgcaggtt gcgattaaag ccctgctggg
     8341 cggtggaaca gaatttacac tccagcgcac accccacctg cgaagagacg cagagcgtgg
     8401 cacggtcgtc ttccgggata tacaccgttt caacgcgctg atcgccaacg gcgatcgccc
     8461 atttaatggt gccgtcagat gaacgctgtt cttcaaccac ttccggtgca cggatttccg
     8521 ccacctcttt cagtttgccg cgcaacactt tattgatgtc ggtcatctca tcaaagttgt
     8581 cgcagcaata gtgatacatc cacttcatca cctgatcggc gcggaagggt ttttcaccta
     8641 aatctttaaa aaactcccgc atctgctgac ggttgagatc cagcaggttg atttttccat
     8701 ctttcgtggt gacgttttca ggtgtgacta attgttcaga catatgctat tccggcctcg
     8761 ttattacacg ttatggcccc tggagggttg aaaaaagaaa cgccccggtg agcggctgct
     8821 cgtccggggg cgctgcattg tacaaattct ggcgcacgga tgccacgttt gcacgcggca
     8881 tttacgaaat tattaacggg tgcgcgggca cacttcgcct tcgccgaaga aataagcgat
     8941 ttcgcgagcg gcagattcga cggaatcaga accgtgggta ccattttcgg tcaggctgtc
     9001 agcgtaatca gcacgcagag taccggccag tgcgtttgcc ggattggtcg cgcccagcag
     9061 atcgcggtga cgctgaacag cattttcacc ttccagcacg gaaacaacga tcgggccaga
     9121 agtcatgaat tcaaccagac catcaaagaa cggttttcca tcgtgttcag cataaaagcc
     9181 acgagcctgt tcaacggtca ggtgcagcat tttggtgccg acaattttga accctgcagc
     9241 ttcaaagcgc gcaaagatac taccaatcac gttttttgct actgcgttcg gtttgatgat
     9301 ggaaaaagta cgttcaatag ccatttttac ctctgtaaat tgttctgttg ttgtctgtac
     9361 cagcgtacga gatggcgcgg attataatga gcaacagggc cgttgactat tgatgaaggt
     9421 aacatttttt taaaataaac ccagtttaag caacaatcag ggacaaaaca gactattgca
     9481 tgacaaattt cactgtcgct atttgcccca cgtcatccat caccagcaat tgataatcgc
     9541 ctttatccgt caaatgcagg gtcacgttgc gcccacgttc agttaacggt tcgccgttca
     9601 gaaaccacca gcgttcacct gcccctccac ttgattgcaa cggcaaagtc gcttccgctg
     9661 cgcccggtaa acgtttaata atcgcgccat cgcgcacacc tgttagctgc agcgggagtt
     9721 gtgcatcgtg accgtatggc gggcaaattg tcgaggccgg aggcaagcgc acagcgcgac
     9781 gttctgatgc gggcagccag ggttccagcg gtagcggcca gacatttatc atttcttgtc
     9841 gcgcctgagg gcaatcagcg gcaacacgtt tgccgttttc atccagccag atggggaaac
     9901 gaatgccatt aatgccttcc tgctccggca gtaatagagt tggcggctgg ctcccgtcca
     9961 gcagccaggt tgccaggcgg cggcgacagt taccgtcacc ttccggcaaa gactgtccgc
    10021 ccggccagca gataacgcca cgacttacag agtcaggacg tgggtcttcc ggcaaattca
    10081 cactgcgcga cagtaagata ttattaacct gattcaacag cggtacggca ctggcaaagc
    10141 caaactgacc aacaacgggc gtgccgtccg gtctgccagt ccagatccca atgacatagc
    10201 gagcgttaac cccaatcgcc caggcatcac gatagccata gctggtgccc gttttccatg
    10261 ccagtggggc gacgcgcggc aaggcaccat ccggcaaggg ttgcgcttca tcagccataa
    10321 tccggcgaat gatccacgca gcccccgacg acattaaagg ccgttcaagc agcggatcgt
    10381 caggctgtaa gcgcaatttg cctgccttgc cgtggcgagc aaacgcggta tacgctgctg
    10441 ccatatcttc cagttttgcg ccagcgccgc cgagaatgag cgaaagattc ggcgcagcac
    10501 cgttgggcag atacaacggc aatccaacat tgcgtaactt tgccgcaaac cgtttcggtc
    10561 cataggcttc cagcacctgc acagcaggta agttcagcga tcgcaccagc gcctcactca
    10621 tgctgatcgg gccatgaaaa ccgctgtcaa agttgccagg tcggtaatca ccggtgcgtc
    10681 gggggacgtc ttgcaacagc gatgccgggt ggatcacgcc ttcatccagc gccagaccat
    10741 aagcaaacgg tttgagcacc gatcctggcg atcggatcgc attgaccata tcaacatgac
    10801 caaagcgtga atcatcgttg agatcaaccg atcccaccca gccgcgaaca cgcatatcgg
    10861 tatgatcaac cacgatcatc gccagtgagc tgcgcggtgg caatcgtcct ttccagtttt
    10921 gcgccagttc ttccagacgt cgttgaagac cggcatccag cgtagtaacg attttgtcgc
    10981 ttttgctttt accgagcatc atgcgcgaaa acagcggtgc cagttgcggc atttgtcggg
    11041 gtgccagcca gatgggttct tcccttgact cttttacccg ctcacgggac cacacacctt
    11101 gcacggccat ccgttcgagc actttattac gcgcggcttc ggcacgctcc ggccaacgat
    11161 ccgggcgaag acggctgggc gcttgcggca acaccgccag cattgccgcc tcggaatagc
    11221 ttaaattcgc aggcgatttc ccgagataag cccaacttgc cgcaccaatc ccctgcaacg
    11281 taccgccaaa cggggcacgg ttaagataca aggtcagaat ttcacgttta gacagatgcc
    11341 attccagttg caacgcgcgc cagagctgac gaattttgcc gccaaacgtt ttggggtgag
    11401 gatcaagcag gcgcgccacc tgcatggtga gcgtgctgcc gccggaaatg acccgacccg
    11461 acgtgagatc ttgccaggct gcacgcgcca ccgagaacgg attcaccccc ggatgcttcc
    11521 agaaccagcg atcttcataa ttgatcagtg cttcaaggta acgtggagaa acatcttcga
    11581 ttgttaccgg ataacgccag atgccgtcag catcggcgaa acgccagagt ggcgtaccat
    11641 cctgcgccac caccacgcgt gcaggattca cttcttgcaa tggcagaggc cagagtttat
    11701 ctgctgccca tgctgccaga atgatgataa aaggcgcggc tgtcagcatt atccagcagc
    11761 cgcgtttggt taacaggcga ggcatttacg gtctgactat caacagatcg tcagccgcac
    11821 cagtcgcccg ccattgagga acatacattg attccaccat aggctgcggc acctgatacg
    11881 ttcccggcgt caccgcccgc gccagataca ccagcgttac cggttgatat tcatcaacgg
    11941 caaccgccgc cacaaagcga tcgtcacgga actctatgtg cttaatgctc gcctgctgca
    12001 tctggttcag caagttctgc acttcaccac cactttgctc caggctggcg ccgccgttcg
    12061 ccagattctg gttttccagt tccagccccg caggcaacag atccacgact aacgcatccg
    12121 gtacactgtt actggcttta acctgcaacc acaccagcac cagatcgccg ctacgtaacg
    12181 agtccagcga tttgctctta ccgtcagtac ccagaatatg acgctcaatt tgcagcacat
    12241 tgctcgccgg taaaggtgcg gattgcggat aaccgctggc atccacacgc agccataacg
    12301 gctgatcgcc actgttggtc acctgcaagg tggcaagttg atcgctattc agattgctgg
    12361 tttgcgcttt ctcgcctgtc agcggctcag cagagaaagt ggtttgcgcc tgccatttgc
    12421 ccggtaaatc ctgaagagta cgggcagcca ggaacaacgc gttactttcc tgcgtcgata
    12481 gccagcgttc accgaacgcc tgctgcgaaa gtgtgttcaa cagggagtac tgctcatcgg
    12541 gtagcagttt gttctcttcc agcaatgaga gcatcaacgc gctgtcgcgc agtggactac
    12601 cgtaatcacc cagccatatc cgctcatcac tattacgcgg cgttttcagc gccagcacaa
    12661 tcgcttcttc accacgcgtt gcatcaccca tgattttcag cgcaacgcca agttgcagca
    12721 gcggtaaacc agaagcggca tctgcacgat gctcccagat ttcacgcagc gcacccagtg
    12781 gtgctttttg ctgacgggcc aacaccagcg cagcgtaaga ctgtacggcg aatttactgg
    12841 ctttgagatt atcagcgtac gggatcgata tcatgcccgg atcttgtaaa tagcgcagca
    12901 gacgctcatt accccggtta atggcgtctg tcggtacgct gtaaccctgt tcgcctgcgc
    12961 ggaccaggaa atccatcacg taagccgtca gccagtactc ttcgtcaccg tttttatccc
    13021 acagcgcaaa gccgccgtta tcacgttgca tttgcagcaa acgggaaatg ccgatatcga
    13081 ccgatgcacg gcgtttctca tcactgtcgc ctttgatgcc caacgcctgc agttgggctg
    13141 cattggtata aagtgacggg aacaggccgc tggcggtttg ctcgagacag ccgtacggat
    13201 acgcttttaa ctctttgata taacgtgcga tgttcagcgg tggtttgccg ctcaacaata
    13261 actgcccttc cagcgtaaca ggcgagaagt tttgcaatcc atcagccggg agcgcccagg
    13321 tttcaccagg ctgtaacgcc gtaccgtaat tgaccgtttg tgccgggaat gccggacgga
    13381 cgccgatttt ccactgctta tgctgatcgg caacggtttc ccccggtaac gctaacccgc
    13441 taatggtggc ctgaatgtca ccatcgccat aacccgtcag agcacgcacc gggataaaca
    13501 gcgtagtacg cacccctggc gctaattcaa caggtgcggg ggaattactc accagttcaa
    13561 gcaagccact ggcggtcaga gcaacattaa gtttttgcgg tttatcggta agattagtga
    13621 tatccagcgt caggcgcgag gtatcgccac tcgccataaa gcgcggcatg ttaagttcag
    13681 caatcaccgg tgcggcaaca atcactttgc tttcgttgct tccgaagtca tctgccgtcc
    13741 aggcttgcgc catgacgcgc aattcaccgt taaaatcgcc aattggcagt gtaaccgagc
    13801 cttcgccctg ttcattgagc gttaccggca gcgcctgctg cgcgacaata ttgacgtgat
    13861 tgaccggcgg tttaccacca cgtttcagct catcaccatc accaccaaaa cgcagagctg
    13921 ccagacgccc ctgaccttca ataacctgac cgtaaatatc gtaaatgtct gcgccatagc
    13981 gtttctgacc aaagaacgcc tgccacggat ctggcgtgac gtaatcagta atattcagca
    14041 caccgctatc aacggcagac accagcacat tcacctgttt tggcatctca ccatttttat
    14101 tgctggcttt aattttcacg gttaatggct gattggggcg cattttggtg ggggtttcca
    14161 gcgccagatc gaggcgacgg ttttcatcac caagcggcag atgcaacacc ccaaccgcgc
    14221 gttttggcgt cgcggagcga gatttatcgc caggacgtac caccagcgta cttaaataca
    14281 gatcatgacg attccaggtt ttatcgaccg gaatcgtcag atccagccct tgagccggaa
    14341 catcaatctc ttgccaccac aacggccctt cactggactc gaccatcgca taacctttac
    14401 ccgccgttgg cgcggcaata tgcaacttaa tggtatcgcc agggcgataa ctggctttat
    14461 ccagtttcag cgtgacacgg tcgggtcgca ctgcaccacc cccgtcgctg ttatcctgcc
    14521 agctataacc agcccagaaa cgcacactac tgaccgcttc attcggcgct ttaacttcca
    14581 gacgataagc gccccactct accgggaaac tgaccttacc ggtttcgtcc gctttcagat
    14641 ccagagtttg ttcattttcg atcagatctt tttgatcaaa ctgtgactgc cagccttcat
    14701 attccgacca gttccagtag taatcgcggc gttcgcgaat caggcgcacc tgcaagcccg
    14761 acacggcttt tttcacgcct tgcgcatcgc tataaacgat gtcaaaagcg gcgttactgc
    14821 cttcatcaac aatgggctgt ttcaccgtgc tgtcggtgcg ataatcgtaa accgatttcg
    14881 aggcgaactg cggacggatc cccggcaatg catcggcagg ccagatagcc tgctcagcac
    14941 ggcgcgtcac cgggcgaccg cccgattcca gcaaactacc ctggaaaata acctgtaatg
    15001 gagaatgtgt ttccttccac tggctttctg tagaaacttc gccgcgccct ttatcatcca
    15061 gcgtcaactg aacttcatcc agcgtgcggg aaagattttc ggcagctata tcgccgaatt
    15121 caaaaccagg taaggctgac acagcttcac gcagtgggcg caggaaaagt tgcccttgca
    15181 aagtattacc attcgcaagt gcaccataca ggtagtaccc caccacggag aatttcactt
    15241 catctttcgg cgttagcggg gttttctcac cggtcagatt cagcgccata cgctctggca
    15301 taaaatcttc gacgtggaaa tcccacatcc gatactggtt atcgcccgtg ttagcgcgaa
    15361 tatgccacat accggttgcc gcattgctat cgagtggcca ggtaaagtgg tagaggccat
    15421 tctccggctg actaacgacg ctcctgagta cctgcccgtc gggtttaatc acgtctaact
    15481 tgatgggttg atcgggcaat gctttaccgt ctgcatcacg cagcaaacca ttgaggatga
    15541 ccgtttcacc cgggcgataa agatcgcgcg ggccaaacat gaaaaactgt ttgctatagc
    15601 ctggcgcgcc agcaatgtta aattccgcta agtccagcgc cgggagtttt aaatcgagca
    15661 gcgttgtctg accgttttta cgcgccagca gtaatgccgc gtttttatca ttttccagtt
    15721 gcacatgccc ctgagcgtca ctggttgcct gagtcagagt ctgccctttc tcatttaata
    15781 aagagacttc aattccttgc agggccgcgc cgttttccag actttgggta aagatatcca
    15841 gacgattgtg ataacggtga gctgaaacgc caatatcact taacgtaaac agcgtcgcgg
    15901 gattactgta atcgtaacgt ccagcctgat tcatcacagc cagatacacg cccgcctgct
    15961 gaagcggttt gatatcgccc agcggcagca ataatttttc acgggtgtta cgcgcaggat
    16021 tgagatcaaa ccgtccggtg tagaccagat ccgccatctg cagcagttta tctgactgcc
    16081 agttcgccag cgaattgcgg tattcccact ggctaatgaa agcgggcagt gattctggtt
    16141 tcacacggaa gaagttaaca tcgacattgt taacgttaag cgccattacc ggcagccctt
    16201 caacgacctt accaggaagc aacgaaccac gactggcaaa accgacgctg ggttggatat
    16261 cgcgggtagt tatagttttt tcgtcatctt tactaaaggt tgcgttattg agcgctttga
    16321 cctccttgcc aatagtgacg atcaaatcac gtttcggttc gaggtggcgt aaacgcagct
    16381 ctttaagatt atctgacagc tcccaggcac catccacttt gccgcttttt ttatcgacga
    16441 catgaataac gcgtgagaaa tcctgatccg ggtcgagagg gatggagaac gtcagcacca
    16501 gcgtggctgc gccatcgagt tgaacttcag agagatccag caatgtcagc gccttaccgg
    16561 cactctgttg ggccagtttt tgtcgctccg gcgcggaaag ttttgctgaa ctcacgtttt
    16621 ctgaagaggc cgctttagta acttcagaag gcgcatcttt tttcaccgct gttggcgcgt
    16681 tatcgttgtt gtcgcaccct gccagcgcca gcattagcat gcaggcggct acgcgtaact
    16741 ttttcatttt tcatccctgc cacaatggcc cgttagcaac gtcgaataat tattatgcgt
    16801 gagaatttac gttatgtaat tcagtatcac gcccatacaa tccgaatttt agtgagaatt
    16861 gtggctcaga cagccaaatc agcctctttt gcttgtcgct tttcactaaa catccgacaa
    16921 tttagcctaa ccccggcaaa aatggagatg cctatgtcca cgacatggtt tgtaggagcc
    16981 gactggctcg ccgaacatat tgatgacccg gaaattcaga ttatcgatgc ccgcatggcg
    17041 tcgcctggac aggaagatcg taacgttgct caggagtatc tgaatggaca tattcccggc
    17101 gcagtgtttt ttgatatcga agcgctttct gatcacactt ccccgcttcc gcacatgctg
    17161 ccgcgcccgg aaacgttcgc cgtggcgatg cgtgaattag gcgttaacca ggataagcac
    17221 ctgattgtct atgacgaagg taatcttttc tcagccccac gagcatggtg gatgctgcgc
    17281 acctttggtg tagagaaagt gtcgattctg ggtggtggac ttgcaggctg gctgcgtgat
    17341 gatctgctat tagaagaagg cgcagtagag ctgccggaag gagagtttaa cgccgcgttt
    17401 aatcctgaag ccgtggtgaa agtaaccgat gtattattgg caagccatga aaatacggcg
    17461 caaattattg atgcccgccc ggctacacgt tttaacgcag aagttgatga acctcgccca
    17521 ggtttacgtc gcggacatat tcccggtgca ttgaacgttc cgtggacgga actggtacgt
    17581 gaaggcgaac taaaaacgac cgatgaactg gatgcgatat tttttggtcg cggtgtcagc
    17641 tatgacaaac caattatcgt cagctgcggt tctggtgtaa cagcagccgt ggttttgttg
    17701 gcacttgcga cgttggatgt gccaaacgtg aaactgtacg acggcgcatg gagtgaatgg
    17761 ggcgcacggg cagatttacc ggttgagccc gtaaaataag ctttttacgg gcaataaaaa
    17821 accgccgaat ttggcggttt tttattgctg gtctggttcg cggcctttcc agcaggttgt
    17881 attaccgtag taatgcaagc gcgtctcagc ggacacaata ctcgccagta actctctttt
    17941 tgtcaagtaa aagagagtta ttattgttct gttagtgtat tatccactgc ggccctttcc
    18001 gccgtctcgc aagcgggcgc tggcttaagg aaaggatgtt ccgcgaccgt tagggcaagc
    18061 gcgtctcagc gcttgctatc gcggcaatat cgccagtggt gctgtcgtga tgcggtcttc
    18121 gcatggaccg cacaatgaag atacggtgct tttgtatcgt acttattgtt tctggtgcgc
    18181 tgttaaccga ggtaaataat aaccgaagtc tcttcggcga caatttactg gtggttaaca
    18241 accttcagag cagcaagtaa gcccgaatgc cgccctttgg ggcggcatat tttagattat
    18301 ccggttctgt ttaaagtcac gcaaaaaacc accccagcga cgttcataga atggcgcaat
    18361 atgttcggta ataaagtggc taattccttt ttcccctttt ttcacctgac aaatatcgat
    18421 gggttcatca ccaggtaatg tatcggtcgc tacacttccc gccacctgaa taatttcttc
    18481 gatatcacca tcggcttcaa tgccaataag taaattaggc tgtgcctctt cgttctcttt
    18541 aattgaacaa ataaaagcac gcttcaccgg cttaatggtt ttaaataagg tggtaagtga
    18601 atcaatcatt tgcgctggcg gctctgcgac ttccgataat atcagcgatt caccgccttc
    18661 cagtacttcc tggctactca gcggattccc ttcttcacca atcaacaaac tgatttcacg
    18721 cggcataaac tctttaccgg ttggcagttt ggcattaagg aagagcgttt cgccaagggt
    18781 catctcaaac aatgtgcgaa cgggcattac gacaaatgcc tgttcgtctt caaccgcctg
    18841 ttgaagtgct tctaacgagg taaaaaaagg aatgacgctg gtgccgtctt ctttttccca
    18901 gtgctgtaaa tcaagcgcgc tatcttcaac tactgcctcg ccctgcgccg ccgtaccagg
    18961 cacccagacg gtggattcca gtagagtacg gaaaaaggcc gggcggtgcg ccggttcggt
    19021 tgctgctttt tccagcaggt cttcaagttc gttttttgtt tcggacataa gaaccacaat
    19081 tcattcaacg ttcggggagc aagtgcctga tgcgccacgc ttatcaggcc tacaagggag
    19141 tcgcaatcta ttggattttc gcaatttgta ggccggataa ggcgtttacg ccgcatccgg
    19201 catattagtt tacgccgtta acagattagc aatcgtgcgc acaccaagtc ccgtagcacc
    19261 cgcagaccac tgttcaaccg gcgctttacg gtaagtcgcc gagcagtcga tatgcagcca
    19321 gccttgctga tagttctcaa caaagtgcga caggaagccc gcagccgtac ttgcacctgc
    19381 cggatacgcc gcgcttccgg tattgttcag ttcggcaaag ttagacggca gctggctgcg
    19441 gtggaattcc gccagcggca gacgccagaa cggttcgttc tcttgtgagg cactcgccag
    19501 caaacgaccg gcaagcgcat cgtcaaaact gaacaacgcg tgataatcat tacccagcgc
    19561 agttttcgcc gccccggtga gggtcgccgc atcaatgatc agttccggtt tctgcgcact
    19621 ggcatcaatc aggccatcgg ccagcaccag acgcccttcg gcatcagtgt tcatcacttc
    19681 aactttttta ccgttgcgat aggtgatgat atcgcccagc ttgaacgcat tgccgctgat
    19741 caggttatcc gcacaacaga ggaacagctt cacgcgcttg ttcagtccgc gcgtaatggc
    19801 aaatgccagc gccccggtaa ccgttgccgc accgcccata tccgacttca tcgagtccat
    19861 aaacgcggtc tgtttgatgc tgtagccgcc ggaatcaaaa gtgatgcctt taccaaccag
    19921 gcacgcgtac actggcgctt ctttatcgcc agttgggttg taatccagcg ccagcaatac
    19981 cggagaacgt tctgaaccgc gcccgacagt gtgcagcccc atataacctt gctcacgcag
    20041 atcttcgcct ttggtgatcc ggtaagtcac acgatcccct gcgacgttgc agatcagatc
    20101 aacagcacgc tgtgccagtt gcgatggtcc cagctcttct gccggtgcgt tgatggtgtc
    20161 acgcacccag tcaatgatca tcaggcggtt atccagttcc tgacgctggg catcgtccag
    20221 ctctggccac tccactttac gtgtgccttt cggggctttg taaccttgcc agaatgccca
    20281 gcagcgatcc gcatcccagc cttcaccgct taactgaaca tgcttaatgc ccagaccatc
    20341 aaccttgcgc gccgcacgct ggatcaaccc cagatcgtct gccccgttca aatgcagggt
    20401 aatgccatca ttattaatgc tgtaagttgc tttttctccc cagcgcacgt cggcaggttg
    20461 ggtagaaagg gtaattttca tcgcttctgt cattttagtt atccttattc gtttaacagc
    20521 aaaacgggct acccccaggc agcccgttca ttcactcggc ttcgtccagc cagaccaaca
    20581 aaatcgcttc gaggattttc tcgttcgatg cctgcgggtc gtcgtcgaaa tcttccagag
    20641 cgcaaatcca ctgatgcata tcggtgaatc gaaccgtttt cggatcaata tcgggatacg
    20701 catcgtacag tgcttcgcca atttcgcggc tatcggtcca cttaagtccc atactaacct
    20761 ctgttaatgc tcacgcgcat ggttgatagt gtaacgcggg atttcgacca ctaaatcttc
    20821 gtcggtaacg cgcgcctggc agcttaaacg gctttccggc tccagccccc aggctttgtc
    20881 cagcatgtcg tcttcctgct ctgagctttc cggcagcgag tcaaaacctt cgcgaacgat
    20941 gcagtggcag gtggtgcaag cacaggattt ttcacaggcg tgttcaatct cgataccgtt
    21001 acgcagcgca acatcgagaa tggtttcacc gctattcgct tccagaacag cgccatcagg
    21061 gcagagatcc tgatgaggca aaataacaat ctttggcata ttaaacctcg tccacggaat
    21121 ggcctttcag cgcacgacga accgactggt ccatgcggcg agcggcgaaa tcctgggttt
    21181 gtttgtctac gtttttaatc gcttgttcga tggcgtcaac atcatcgccc tgcgccactt
    21241 cactcaggtg agcggcagca tcgtcgataa cctgacgttc tgcggcgctt aacagcgcgg
    21301 catcagcagc cagcgcaccg tgcagacttt ccagcacacg cgccgcttct actttttgtt
    21361 ctgccagcat tcgggctttt acgtcctgct cggcatagct cattgagtct ttgatcatcg
    21421 aggcgatttc gctatcggtc aggccataag acggtttgac ctgaatagac gcctcaacgc
    21481 cggtggattt ctccatcgcc gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca
    21541 cgcgaatatg cgcaccgcca gccggtagcg ccggaatacc tcgcagcgca aaacgcgcca
    21601 gcgagcggca gtcctgcacc agttcgcgct caccctgcat cacatggata gacatcgccg
    21661 tctgaccatc tttaaaggtg gtgaaatcct gggcgcgggc caccggaatc gtggtattac
    21721 gcgggatcac tttctccacc aggccgccca tcgtttcgag gcccagcgac agcgggatca
    21781 catccagcaa cagcatttcg ctgtccggct tgttacccac cagaatatcc gcctgaatcg
    21841 ccgcgccaat agcgacgact ttatccgggt cgatagaagt cagcggtgga cgaccgaaaa
    21901 attcgcctac tcgttcacgc accagcggca cgcgagtaga accgcccacc atcaccactt
    21961 ccagcacttc atcagcttct acgcccgcat ctttcagcgc gcgacgacaa gccagtaagg
    22021 ttcgttttac cagtggcgcg atcagttcat tgaattgttc acggctgatt tcgccctgcc
    22081 agcccgcaac gttaacggtc acagagtccg catcgctcag cgcgattttg gccgcaatgg
    22141 cagcatcgag cagttcacgc tgaacgcggt tatcgctacg atcaggaatg cccgcctgct
    22201 cgcgaatgta atccgccagc agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc
    22261 cggtcgccag cacttcaaac acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc
    22321 cgccgccgag gtcataaaca gcgatcacgc cttcctggcc ggaatccagc ccgtaggcaa
    22381 tagccgcagc ggtcggttca ttaagtaagc gcagaacatg cagccccgcc agacgcgccg
    22441 cgtctttggt gccctgacgc tgggcatcat caaagtacgc cggaacggtg ataacgacac
    22501 catccagctc gcctgccagg gcttcagttg cccgcgccgc cagtgctttg aggatgtccg
    22561 cagaaacgcg caccgggttc agcagccccg ccgccgtttc aatcatcggc aggccattct
    22621 cgctggcctg gaattgataa ggcagatgcg gatagcgttg ctggatatca gccagcgagc
    22681 gccccatcag gcgtttaaca gaactaatgg tgttggcggt gtcgagcgct gcgttagtac
    22741 gcgcgtcata acccaccgaa tgcccttgct gttgatagtg aacaacagac ggcagcaggt
    22801 gacggccttc atggtcagct aacgtttcgg cctgaccgct gcgcactgtt gccaccagcg
    22861 agttggttgt gcccaggtca ataccggccg ccaggcgacg ctgatgcggc gcagcactca
    22921 aaccaggttc actaatttgt aataaggcca tgtttagctt ccagaaatta aaaatcgagc
    22981 agtttttctt cgagttgttc ggcactgctc cgcagtttat cgagaaaacg cagcttacgc
    23041 acagtatccg ccgccgcgtc ccacgtctcg ttgtctaact gttcaaccat caactgatgg
    23101 cgggtatcaa acatcttttt cacacgtttg ataaagcttt ccagccgcgc ttcatctttc
    23161 gcctgttcga tctcgtccag ttcttcgcgc agttccagct gttccatcag gaacgcggtg
    23221 tcgcgcacag tatgctgctc gctggcgaga tcaaaaccgt gcaaagaaag caaatattcc
    23281 gcgcgcatta acggatgacg cagcgtttgc caggcctggt taatggtcgc ggattgctgt
    23341 acggcggcga gttgttccgc ctggcttccg ctggcgaatt tatcaggatg atactgacgt
    23401 tgtagatcct gaaaacgcag gctcagcgcc tgggtatcga gttgataacg ggcaggcaag
    23461 ccaaagaggg tgaagtaatc cataacattc tcagggctgc gatccgccgc gataacggcg
    23521 aatcaggtaa aacaaacccc acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc
    23581 aaacgtggaa gctttcgccg caaccacact catctttgac gttcgggttg gtgaatttaa
    23641 acccttcgtt aaggccttct ttgacgaagt ccagctgcgt accgtccaga aattgcaggc
    23701 ttttgccatc gaccacgact ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg
    23761 gttcgtcaac aaattccagt acataagcca tacctgaaca cccggaggtt ctcacgccca
    23821 gacgcaggcc aaacccttta ccgcggttag ccagaaaggt atttactcgc gctgctgcac
    23881 tgtcgctcag tgtaatcgac ataaccaaac ctcaactctt attttgcttc acgtttgctt
    23941 ttatagtccg caatggcggc tttgatcgcg tcttctgcca gaatagaaca gtgaattttc
    24001 accggcggca gttcaagttc ttcagcaata tcggtgtttt tgatcgcctg cgcttcgtcg
    24061 agagacttcc ctttcaccca ttcggtgacc agggagctgg aagcgatagc ggaaccgcag
    24121 ccgtaagttt taaaacgcgc gtcttcaatg ataccttcat cgttgacttt aatctgcaac
    24181 ttcatcacgt cgccacaggc cggtgcaccc accatgccgc tgccgacgtt ctcgtcgttg
    24241 ttgtcaaagg aacccacgtt acgcggattc tcgtaatggt cgataacttt ttcgctgtaa
    24301 gccattataa attctcctga ttccgatacc gattaatgat gagcccattc gatgctgttc
    24361 agatccacgc cctgcttgta catttcccac agcggagaaa ggtcacgcag acgaccgatg
    24421 gatttacgaa ctaactcgat ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa
    24481 gagaaacgga tagagctatg tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag
    24541 gacggttcga ggcttgctga cgtacaggcg gaacctgaag aaaccgcgag gtctttcagc
    24601 gccataatca gcgactcacc ttcaacgtag ttgaagctga cgttgagaat gttcggcgca
    24661 ccgtgttcca ggtcaccgtt caggtaaact tcttcgatat ctttgatgcc gttccacaga
    24721 cgattacgca ggccgcgcag acgttccatc tcggtcgcca tctcttcttt tgcgatgcga
    24781 taggcttcgc ccataccgac gatctggtga acaggcagag tgccggaacg cataccgcgc
    24841 tcgtgaccgc cgccatgcat ttgtgcttcg atacgtacgc gcggtttacg acgcacatac
    24901 agcgcaccaa tacctttcgg gccatagatt ttgtgaccgg agaaagacat caggtcaact
    24961 ttcaactggc tcagatcgat cggcagtttg cccacgctct gggttgcatc aacgtgatag
    25021 ataatgccac gagcacggca catttcgccg atagccgcga tatcctgcac cacgccgatt
    25081 tcgttattta cgtgcatgat ggaaacaagg atggtgtcgt cacgcatcgc tgcttcgagt
    25141 tctttcaggt cgataatacc gttacgctgc ggtgccaggt aagtgacttc aaaaccttcg
    25201 cgctccagct gacggcaggt atccagtacc gctttatgtt cggttttact ggtgatgatg
    25261 tgcttgcctt ttttctgata aaagttggct gcacctttga tcgccaggtt gtcagattcg
    25321 gttgcaccag aggtaaagac gatttcacgc ggatcagcgc cgaccagatc ggcaatctga
    25381 ttacgggcga tatctaccgc ttcttcagcc tgccagccga aacggtgaga acgggaggcc
    25441 gggttaccaa aggttccgtc catcgtcata aactgcatca ttttctcggc aacacgcggg
    25501 tccaccggcg tggttgcgga gtagtcgaga taaatcggta atttcattgc tctataaact
    25561 ccgtacatca ctcaatgcaa ggaaccaggc taccggctgg atgtacgacc gtgtttacgg
    25621 agtgtttagc actccggcct gattctgaat tctttttatt aagcgcgtaa cttaacgtcg
    25681 atcgcgtctt gtgtgcgggt gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc
    25741 agcacttcct ggttattaac cagttcgcct aaagtaatgt tattgagaaa accggtaaga
    25801 cggtcgctca aatcacgcca cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg
    25861 cctttaccct gacaacgggt ggcatctaca gattcgtcaa cggcgctaat cacctcgcca
    25921 acggcgatgc tgctggcatc tttgcctaac agataaccac cgcctggtcc acgtacgctg
    25981 gaaaccagac catttttacg cagacgggaa aacagttgtt ccagataaga aagggaaatt
    26041 ccctgacgtt cggaaatatc agccaacggt accgggcccg cttcagagtt gagcgcaacg
    26101 tcaagcattg cggtcacggc atagcgccct ttagatgtca gtctcatgtc ttacttcacc
    26161 tcaaactcgc ccctgcccgg ggttttttat tgtaaaagtg ggggtattgc atagcagggt
    26221 caagtctgac attcccgagt aaattggtca actatttact tgactgattt agtcgggtat
    26281 ttaaccttca gtgccatttt tttatcgtgg cgtttgtacc tgttgtcgga tgcggtgtga
    26341 acaccttatc cgacctggga ttccgtgcac ttacaggcct gataagacgc gttaagcatc
    26401 gcatcaggca accactgtta ttcggccttg ttacctttat tctgctgctc aatagaagcc
    26461 agaatcccgc gcaggatatt caattcctgg ctttccgggc gcgcacgggt aaacagacgg
    26521 cgcaatttat tcatcacctg tcccggatgg ttttcacgga taaaaccggt tgccagcagc
    26581 gtttgctcca gatgaccata aaaacgctcc agatcatcga ccagcggata cggcgtctct
    26641 tcatgctcga cctgctcgcc attttcctga gttgccagcc aggccatgcg cacttcatag
    26701 gcaataacct gaaccgccat cgccaggttc agcgaactgt attccgggtt agccgcaatt
    26761 gcgacatgat aatggcattt ctgcaactct tcattggtca agccgacgcg ctcgcgacca
    26821 aacaccagcg ccaccggggt atttgccgct tcagcgacgc ttttcagacc gcattcgcgc
    26881 gggtcgagca tcggccacgg cagcgtgcgg gagcgagcgc tggtgcccac caccagacta
    26941 caaccggcta acgcttcgtc gagcgtatcg acgatgtgag cattaccaat cacatcgctg
    27001 gcccctgctg ccagggcaat cgcctgggag tcaggtttca ctagtggatt aaccagccac
    27061 agattggtta atcccattgt tttcatggca cgggcaacag aacccatatt gccggtgtgt
    27121 gacgtctcca ccagcacaat tcgaatattt tgcagcattg tctttcttca tctaaagatt
    27181 attcacgcat cttatcataa aacgaagaca gatgccgatc tcgctgctat actctgcgcc
    27241 gttttcccgt tctttaacat ccagtgagag agaccgatgc atccgatgct gaacatcgcc
    27301 gtgcgcgcag cgcgcaaggc gggtaattta attgccaaaa actatgaaac cccggacgct
    27361 gtagaagcga gccagaaagg cagtaacgat ttcgtgacca acgtagataa agctgccgaa
    27421 gcggtgatta tcgacacgat tcgtaaatct tacccacagc acaccatcat caccgaagaa
    27481 agcggtgaac ttgaaggtac tgatcaggat gttcaatggg ttatcgatcc actggatggc
    27541 actaccaact ttatcaaacg tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa
    27601 ggccgcaccg aagttgctgt ggtatacgat cctatgcgta acgaactgtt caccgccact
    27661 cgcggtcagg gcgcacagct gaacggctac cgtctgcgcg gcagcaccgc tcgcgatctc
    27721 gacagtacta ttctggcgac cggcttcccg ttcaaagcaa aacagtacgc cactacctac
    27781 atcaacatcg tcggcaaact gtttaacgaa tgtgcagact tccgtcgtac cggttctgcg
    27841 gcgctggatc tggcttacgt cgctgcgggt cgtgttgacg gtttctttga aatcggtctg
    27901 cgcccgtggg atttcgcggc aggcgagctg ctggttcgtg aagcaggcgg catcgtcagc
    27961 gacttcaccg gtggtcataa ctatatgctg accggtaaca tcgttgctgg taacccgcgc
    28021 gttgttaaag ccatgctggc gaacatgcgt gacgagttaa gcgacgctct gaagcgttaa
    28081 tgactcaggc gggtgatatc actcacccgc cctcgccttt caggcgctat tccgaaatac
    28141 ttcctcaccg ctttactttc tttcgtcact ctcccaccat ctttcgccga ttaatggatg
    28201 ataaagaagt cgcaaggaaa ggttatggcg ctgccagtga acaaacgcgt tctcaaaatt
    28261 ctgtttattc tctttgttgt tgccttctgc gtttatttag tgccgcgcgt cgccatcaac
    28321 ttcttctatt atcctgacga taaaatatac ggtcccgacc cctggtcggc ggaatccgtc
    28381 gaatttacgg ctaaggacgg tactcgtctg caaggctggt ttattccttc ttcgacgggc
    28441 cctgctgaca acgccatcgc aaccatcatt catgctcacg gcaatgccgg aaatatgtcc
    28501 gcccactggc cgctggtcag ttggttaccc gagcgtaatt tcaacgtttt tatgtttgat
    28561 tatcgcgggt ttggtaaatc aaaaggcacg ccgtccctgg ccggattgct ggacgatacg
    28621 caaagtgcca tcaatgtggt gcgccatcgc agtgatgtaa acccacaacg gctggtgctg
    28681 ttcgggcaga gtattggcgg ggcgaatatt ctggatgtta ttggtcaggg tgatcgtgaa
    28741 ggcatacgtg cggtgatcct cgactccacc tttgcctctt atgcgaccat tgccaaccag
    28801 atgatccccg gcagtggcta cttacttgat gagagttaca gcggcgaaaa ttacatcgcc
    28861 agcgtcagcc cgatcccgct tttactcatt cacggtaaag ctgatcacgt tatcccatgg
    28921 cagcacagcg aaaagttgta tagcctggca aaagagccaa aacggttgat cctaatcccg
    28981 gatggcgaac acattgatgc tttttccgat cgtcacggcg atgtttatcg cgaacagatg
    29041 gtggatttta tccttagcgc attgaatccg cagaactaac ccatgatcgc tagcacgata
    29101 atcattcaca aaaccacctt aagacatgct aatccactgg tcagaacagt ttaagatgag
    29161 aaaaattctg tgatgcttgc caacatttct gatgattagc attcccttcg ccatttccct
    29221 gagcaaactt tagctattct tatcaattat gcttatggga gatctacaga tgatgcctac
    29281 gcttgctcca ccatctgtcc tttcggctcc ccagcgccgc tgccagatct tgctgacgct
    29341 ctttcagccg gggttgaccg ccaccacggc aactttcagc gagcttaatg gtgtggatga
    29401 tgatatcgcc agtcttgata tcagcgaaac aggacgggag atcctgcgct atcatcaact
    29461 cacactgacg actggttatg acggcagcta ccgggttgaa ggtacagtgc ttaaccaacg
    29521 tttgtgttta tttcactggc tacgacgtgg tttccgtctg tgtccgtcat ttattaccag
    29581 ccgattcacc cccgccctga agagtgaact gaagcggcgc ggaattgcgc gtaactttta
    29641 cgacgatacc aatctacaag cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt
    29701 tgaaacgcgc gatattcatt tcctgtgtct gtatttgcaa tattgtttgt tgcaacatca
    29761 cgctggaatt acgcctcagt ttaatccgct ccaacgtcgc tgggcggaat cctgccttga
    29821 atttcaggta gcgcaggaaa ttggacgcca ctggcagcgt cgggcgctcc agcctgtacc
    29881 gcctgatgag ccactattta tggcactact tttttccatg ttgcgggttc ccgatccact
    29941 gcgggatgcg catcagcggg acagacaact gcgtcagtct atcaaacgtc tggtaaacca
    30001 ttttcgtgag ctgggaaatg ttcgttttta tgatgaacag gggttatgcg atcagcttta
    30061 tacccacctc gcccaggcgt taaatcgcag tttgtttgcc atcggtattg ataacaccct
    30121 gccggaagag ttcgcccgcc tgtatccacg cctggtgcgc accacccgcg cggcgctggc
    30181 cggatttgaa agtgaatacg gcgtccatct ttctgatgag gaaagcggtc tggtcgcggt
    30241 gatttttggt gcctggctaa tgcaggaaaa cgacctgcat gagaaacaga ttattctact
    30301 gaccgggaat gatagcgagc gagaagcgca gattgagcag cagctacgcg aactaacgtt
    30361 actgccgctc aacattaaac atatgtcggt aaaggcattt ttgcagacag gcgctccgcg
    30421 cggcgcggca ctgattattg cgccttatac catgccgtta ccgctctttt caccaccgct
    30481 gatctatacg gacctgacgt tgacaacaca tcaacaggag cagatccgca aaatgctcga
    30541 atcagcatga gggaacaact ttcgggcgca aaaacattgc cggaagcgcc accagcgcca
    30601 tcacccagaa cacgccgtgg ccgagatatt gatacaggaa accggcgaaa acggtcatga
    30661 tggcgatact gccgcccatc gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc
    30721 taccctggcg agcagcaata tagcgcatgg cagccaggtg gcagaccgtg aaggtaccgc
    30781 aatgcagaat ttgcaccact atcaaccacg gcaacgccgt cgttgctccc ataatgcccc
    30841 agcgcactac gccgcaaatc gccgagatca acagcatatc gcgtgcacta caacggcgga
    30901 aaagtttatt actcagcgca aaaataatga cttccgccac cacgcccagc gaccacaaat
    30961 accccaccgc cgaggccgag tagccagctg cctgccagta aatggcgcta aaaccgtaat
    31021 aggccgcatg tgccccctgc aataaacaaa cgcaggccag aaagcgccag ttctggcgaa
    31081 ccaacgccaa ccacgcagac cagccggtgc tctcctgctg gcggcttgcc ccttgtggct
    31141 gaatcgtcgg acggatgaga aagccgagca gcatggatgc cacgcccaac gtcaacagcg
    31201 cgaggatcac ccgataatca aacatactga ccagtttgcc cgtcagcgcc gagccaatga
    31261 caaacgccac cgagccccac agtctcactt tgccgtaatc aagcgggaac tgcttttgcc
    31321 acgtattcgc cagtgcatcg gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa
    31381 tcatcaccag cattagccac gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa
    31441 gtgtcagcag tgccagcacg cgcaaggcgg aaatcaggcg ggaaggatcg ctgacgcggg
    31501 gcgcgatgag caaactcccg aggaaacggg caaccagacc cgcccccaat aacaggccga
    31561 tggtttctgg cgttaaacca atccctttaa gccagacgct ccagaaaggt agaaaaatgc
    31621 cgtaactaaa aaagtatgtg aaatagccga gcgccaacca gcgcgtggat tgcaaaacca
    31681 tgagtccctc ccgtcaggag gcgttagtct ggggttaatc gaatgttggt gcaagttgaa
    31741 aaataagaat attcgagcct gcttcctgac ggttatcctg catatcagga aatgacaaaa
    31801 aagtgcggcc cacaacacaa catgtaaaaa agaacgatga aacaggtgaa aatcgcaatt
    31861 tattgaactg acaaactcat gtgagacgga taaggcgtgc atgtcgcatt cggcaggaaa
    31921 caataagcac attgtcagcc atctgcgtta tgccgttata ccagaaacga atatctcaac
    31981 gcctggggcg tggcgaggta ccagagtgtg tattacgttt aaatcacatc atcttgcaaa
    32041 gggattggtt atgaactcac tacgttattt tgattttggc actgcccgcc ccgttttatt
    32101 attaattgcc cgtatcgccg tggtcttaat tttcattatt tttggttttc ccaaaatgat
    32161 gggctttgac ggtacggtcc aatatatggc ctcgctgggc gcgccaatgc cgatgctggc
    32221 agcgattatt gcggtagtta tggaagtgcc cgccgcgata ttaatcgtgc ttggcttttt
    32281 cacccgtccg ctggcggtgc tgtttatttt ctacacgctg ggtacggcgg tgattggtca
    32341 ccattactgg gatatgaccg gcgatgcggt tgggccaaat atgattaatt tctggaagaa
    32401 tgtcagtatc gctggcgcat tcctgctgtt ggcaattacc gggccggggg cgatttctct
    32461 cgatcgccgt taggaaagat gccggatgtg gcgtgaacgc cttatccggc attttataaa
    32521 ttacagcaac gcaacccgca acgacatctc tgaaattgtt gattcccctg gtgcaagcgc
    32581 aatgaggtca ccaccttccg ggcgatgatg gtcatccggc gcatgactca tcggttccag
    32641 acagaagaaa tcaaacgcat atcctttatc aaacgcaggg tctgaaacaa agatgaaata
    32701 acacggtgca ggtggcgtcg tttccatgat gatggcatac ccctcctgcg gctgctcgat
    32761 gcgggcctta ccattccatc cggcaaaacc attgtttacc cactggcgcg gcaacggcgc
    32821 gagctggtta aaatccagct cctgcggtag ctgctcgcaa aactcacccg ccagccactg
    32881 ctcccgctcc agccagtaac cgctcgcctg tgcctgaatc cgcgtttgcg gcgacaacgg
    32941 aaaataagga tgccagccgg taccaaatgg cagcgtctct gccccttgat tggtgacagc
    33001 gagcgtcacc gtcagcgtat ccgccgttaa atgaaatgcc tggcttaccc gatagtgata
    33061 aacaccgctg agatgttcat acaccagaca caagctatca tcgctgcgcg agacacattg
    33121 ccactcgccc aaccagccat cgccgtgtag atagtgcgca tcccactcaa cattcggttg
    33181 cagttgatac tcacgcccct gccagacaaa acggttgccg ctcacccggt ttgcaaatgg
    33241 taccagcgga aaacaagagg catcggttgc cacaccgctt tttttaccag gacgtagcag
    33301 cggcgtcgta tcgcgccaga agccttcgat tacgccgccc tgatcagaaa cgtccagctt
    33361 tagcggcccg tgagataagg tatagatggt catccgcaga ctccttaatc cgggaaatta
    33421 atcacaactt tcccacattt gccgcttgcc atcagtgcat aagcatctcc tgcctgttcc
    33481 agcgagaaac gatgggtaat ggcgttacgc ggccacagtt tccagtccgt taaatcgtgg
    33541 gcgcattttt ccatatggaa caaactggtc acccaggagc caataatccg ccgttgatgg
    33601 tgcatcaggt cagcgctaac ctcgaactcg acttttccgg tttcaccaat gtaaaccacg
    33661 cgtccccagt cagctgtgga ttgcagtgcc agtaagcgac ctgcggcatt accggaacaa
    33721 tcaagcgcaa catccgcgcc gccgtgagtg agttcggcga ttatctgcgg cagaccttcg
    33781 gtggtcgcta aatagccgtg atccatcact cctaactgtt tcgccatcgc cagacgttcc
    33841 ggcagcatat caacgccgat gatccttttt gcaccacgac ctttcgccag catcatcgcc
    33901 atcatgccga ccggccccag accgaccacc agcacgttat cgctaccgga aacttcgccg
    33961 cgcaaaatcc cttcatacgc tgtaccaacg ccgcaactga taaacgcacc atcttcatag
    34021 ctcagcgcgt ccggcagcag gatcagatct ttttcttccg ccagcaggta ttcagcatgg
    34081 ccgccgtcac gctgccagcc gtaagccgct tttccttcgc cagtacaaga aataggaaaa
    34141 ccgcgacggc agttcgggca aaaaccacag ccagaaatgt gatacaccag cacgcggtcg
    34201 ccctctttaa aatggcggca gccttgcccc atcgccacaa tctgcccgca cggttcatga
    34261 ccgttgataa atccctggta taacggttta tcgggtgccg ccgccgtagc gcggtgctgg
    34321 tggtagatat agtggacatc gcttccgcaa atcccggagg atttcatttt gatcagtacc
    34381 tggttaatcc ccggcgtcgg caccgcaact tcccgcagat cgacggtcga atttcctggt
    34441 aaataagctg ccagcatcgt tttcataaat ccctcattaa caatacgatt aattttcatc
    34501 cctgccgcac ccgcgccagg ggcgttaagg tttagcgttt cgctttacta ctacgctggg
    34561 tcagcaagat atttgccagc accgccacca caatgatgac gccgcgcacc acctgctgga
    34621 aaaaggagtt aatcccgagc agcaccagac cgttaccgat tagcgtaatc accagtacgc
    34681 caagcagcgt accgaacaac gagccgcgac cgccggaaag cgccgtaccg ccgaccacga
    34741 ccgcggcgat gacgtcaaac tccagaccgt ttgcggcacc tgcgttaccg gaaccgaggc
    34801 gcgccgccaa caaaatgccg gtcaccgccg ctaataatcc cgaaagggta aaaataagaa
    34861 tgcgtacccg acgaacgtta ataccgcaca actgcgctgc agtggcatta ccgccaacag
    34921 aaaaaaccga gcgcccgaag gcggttttgc ggctaatgaa cacaaacagc gcaaataaca
    34981 ccatcatgat cagcgcggat accggcacac cgagaaattg tccgcccagc cagtccagca
    35041 cttcgttttc gttaattggc actggcaagg cgttcgtcat aaacagcccc attccgcgca
    35101 gggcgctcca taaacccagc gtggcaacaa aacttggcac gttaaacacg ccgcgcagca
    35161 ccccggcgag cgttcccatc agcgcaccta acagcaacac cagcagacaa gcaatcgcca
    35221 gcggaacttc aaattgcagc aaaaacgcca ggcacactga aacaaaagcc accatcggcc
    35281 caacgctgac atcaatttca ccggagataa taatcagcgt catcgcccag gcggcgatcc
    35341 cgatggtggc ggcatcgcgc agcacgttca tttggttatt caatgagata aagccaggcg
    35401 cattcaggga gaagaccaga taaagaatgg caatcaccac cagcaaaccg atctcattaa
    35461 tatggcgact gacaaattgt ttgagcgaga cgctcttgcc ctgcggcaat ggtaatgacg
    35521 aagcagacat cttcaggttc ctcggtaaaa ttcatcagtg gacagaatgg cggacatcag
    35581 ctcatccaca ttgaccggcg agtgaaactc ctgcgagaac gtgccgtgct gtaataacag
    35641 gatgcggtca cacaccagcg gcaactcctc cacttcactg gagataaaca ccacgctttt
    35701 tccttcggca gccagctcgc ggacaatacg gtaaatctgc tgtttggctt cgatatcgac
    35761 gccgcgcgtt ggctcgtcga gcaacaaaat ctggctggcg gcatagaccc aacgaccgat
    35821 caccactttt tgctgattgc cgccagaaag cgtgccgatg ggcgtttcgc tactggcggc
    35881 cttgaccgtc atccgctgca tcacctcttc ggtcaggcgg cggatggtgg accattgcag
    35941 cacaccgttg gcgctgattt tttgccgatt ggtcagcact gtattttcgt caacgcccaa
    36001 ccagggaatg atccccgctt ctttgcggtt ttctggcgta tagccaatgc cgcgtttcag
    36061 catgtcgccg taatcggggc gcatgatttt ctcgccgttg ataacaattt cgccctgttc
    36121 atacgtctcc agaccaacaa tcgccttcag caattcactg cgccctgccc ccagcagacc
    36181 agcaatgccg agcacttcgc cacgacgtag cgtaaaactg atatcctcca gcttgggctt
    36241 atggcgtaac gcacggactt ccagcacggc ttgatccata atttcctgag gggcaaccgg
    36301 cgcaatatca acgtgatcgc gcccgagcat tagcgacaca atatgatgcg tagaggtgtt
    36361 ttcgagcatc acatcgcccg ccacctgacc atcgcgcata acggtggcac aggaggcaat
    36421 gcggcgaatt tcttccatcc ggtggctgac ataaatcacc gccacgccca gtgccgacat
    36481 ctttttcacc gcgctgatca ccagttcaac ttccgcactg gcaagcgagc tggtgggttc
    36541 atcaagaatg accacgcgcg gctcgccctt catcacccgc gcaatttcca ccagttgctt
    36601 ttgcgccggg cttagcgttg aaacaagttg ttcagggcta acgtcaacgc ccagcgcctg
    36661 taagcaacgt tgggcatcct gcgccatttg caggtaatca atcatgccgt tgcggcgggg
    36721 ccactgaccg aggcagaggt tttccgccac tgtcagccct tccaccagac ttaactcctg
    36781 ataaaccgcg cgtaccccca gttcagcggc acggcgagtc agcgtagctt cgtcaccttc
    36841 cagtcgcgtc tcgccaatcc agatatcacc gctatccgga cgttcgctac cggtaagcat
    36901 tcgaatgagg gtcgattttc ccgcaccgtt tttgcctaac agcgcacgaa cttcgccttt
    36961 tttgagcgtg aagttaacgt tatccagcgc aacgactccg ggataacgct tatttcctgc
    37021 caccactttt gctaccggga ctgcctctgt tgccgtgaac atagggacct ctgcgaatca
    37081 gcgattaggg cagaccatca acgtgcgttg ccagccactg tttaccgtct tccgttttgg
    37141 tatagagatc gattggcacc tgaatcactt tttcaccgtc ggcttgttta ttgataacct
    37201 tcaatgtttg cgtaaaaaca gcattgccca ttttcttacc agaaatatcc accaccgctt
    37261 tcagcacctg gttgttttcc agctcctgag caatttcagt ggtcatatca gaaccgaaaa
    37321 cagcaatttt tccggcctga ttttgattac gtaccgcttt taccgcgccg agtgtcgctc
    37381 cgcccgattc ccccataatg gcgttgagat ccggcgtgga gataatcagt ttttcaccga
    37441 cagaaatcgc tttatctaaa acagtccctt cctgattagc gacaatttgc gcgccgggaa
    37501 cgcgggtttt taatacttct tcaaaccctt tacgacgttg cacacaaact tcaaaggctt
    37561 cgcaattgat gacggcaatt ttcggctggt caattttgtt ggcaataaaa taatcggcgg
    37621 cagcgttacc cagtttttta ccaaattcca gcgggtcacc aaccagatat gccgagacat
    37681 atttatcgac acccttttga ttaatacagg tgttgtagca aatcaccgga atacccgctt
    37741 cactggcgcg acgaacggta cggctactgc cattttcaga cactgccgat aaaataatgg
    37801 catcgacatt acgcgccacg agggtatcaa caaaggtact ttctttcgaa atatcgccct
    37861 gagcgttagt ttcaattaac tgtacctgta ctgaagaatc ttttgccgca tcctgaacgc
    37921 cctgacgcac tccggcgtaa tatccctggg tatcaaggta tattgcgcca atagtcattt
    37981 ctttatcaga agccctggca aatagtgcgc tacccaatag tgtagccatt aataataaat
    38041 tacgggttct tgtcattttt ttaggcatag tgcctcctgt agggttttta tttacaacgg
    38101 cttattttaa ttatttgagc gtgagcggca gcacgtgccg ctcaaatatt acagttaata
    38161 gggtttagtg aacgtagcta aaaataaaag gcatcacggt ggtgaataat gccgctttcg
    38221 gccaggctgg attaatcgcc atcaattcag cgcgagcaga ttcatagtgc gcaacctccc
    38281 ctaatcccgc ggccgccagc atcgccacca tcaggcattt ttctttatgc tgctgttgta
    38341 aatcgccata cagcgacaac aagtcaggct gcgagacggc aaagaaatcc gcctcgatac
    38401 tggttttcgc catctcttgt gcccactgtt tcatttcact aaacagttgc tgtgcagttt
    38461 gttgttcgcc cagcagtcgc agcgccattc cttgccagaa gagataatca accggctgat
    38521 cgttgtaata actgtggatg ttaatggtgc gatcgccggt cgccgccaga cgtaaacaac
    38581 gcgtcgcttc agtttcatcg ccctgcgcgt tggcgcatac tgcctgccag aaccagatgt
    38641 cgttgtcagt ttgccccggt aaacggcctt cgcttaaatt ctccggataa tgcagcgcgg
    38701 catgaagcag ttcgctggcc tgctgcggct ctctgtcatc aagatgctgc caggcgcgta
    38761 ataactggtt gaggataaac tggctggtga ccttcccttc cccaccttcc cacgggtgga
    38821 atttgcgcgt ggcgagaatg tccgccgctt tgtctgcctg acccgtgaga tgccacaaat
    38881 tgagcagttc tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta tcttccagac
    38941 gcgccagtcg tttctccggt gtgacaccgc ttaacttatc cagcaaatcc cgttcgaaaa
    39001 gcagacgtgc atcctgcggc gcaagctgat aagcattatc aagataacgc gcggccagct
    39061 cataatcgtg ctgcttattc cacgcatgga tcgccaaccc gcgccagccg tcggcaaact
    39121 ccggcgacat ctctacgcaa cgctgccata aggcaatggc tttgccgtag ctacgcttgt
    39181 tgtagtagaa gcaggccagt aaatgccggg caaaccagca ctcttcgata ctctccagcg
    39241 ccgccacttc ttccagcgta ttcgggaaac ggacaaactg cgggaaggca tcaatagctt
    39301 ttgcgaccag ttctccgcgt tcagcttttg gtagcaaact ggcttgcagg taaagcggta
    39361 gcgtgcgctg acagtccagt gcgttcagca tatctgccgc cagggcgggc atgccccagt
    39421 taatcagttg cccggcggtc atcagggcgt taacgtcgcg tccctgacaa agacctcgcc
    39481 actgcgcgag ggctgattcg ctacgaccat cgaaccagtt cagccaccac agagtggcgt
    39541 tcagcggata atcacgcagc agtttctcgc gctgcaaacg cgcgttgtcc tgacgaccac
    39601 ttaacaccag tagcaggtta tgcaggcaaa gcacttcctg attggttggg caggcgcgaa
    39661 gactttgttg gcaaaaatcc agaccagcgt cgaagttacc attacgcgcc gccagtcgtg
    39721 ccagaccata atagccgccc gctttactgt tgccgctcca gaccgcacgc cagaaatcct
    39781 cttcggcttg ttgatattgc ccctgacgtt cgtaagcact ggcgcgaatc aaactcgcct
    39841 gtccgcactg cggatttttg ttcagcgcat gtgcgcgttt cagtgcctga ctggcatata
    39901 ccaccgcttg cgggaaatct gcacggttat attccagcat tgccagcgcc agattacagc
    39961 gataatccag cggatccagc gccacgccgc gcaggtagta atcgaacggt gaacggctgg
    40021 cgtgatgata ttgctccaga tgttgaccga taaaccaggc ttcatctgta ctggtaatgt
    40081 cttgtgctgc cagtggcgct ttggcgacgt ccggcaacgg caacgcttgc ggctgatgtt
    40141 catgataact cagtacaata ttgccgtcgg catcggagag ctcaatggtc agcctttcag
    40201 gattgatacc gtgcaacacg ccctggatgg cggtcgctgg tgtcagggcc acggcatcat
    40261 cgagcaacgc gttgcatttg ccgatttcgc ggatcgccag gcggtatccg ttcaacggag
    40321 agatggcata cagcccccac tcaatccccc gctcactacg ctggagtttt atcaccgcat
    40381 cgcgggaggc attttgcacc atgcccagag aatgataagg caggaaatac tgctcgaaac
    40441 gcttctcctc gtaagcatca agccaggtaa aatcaggctg gttatcggca aaaataccgg
    40501 tcatcagttc gatatacggg ccgttattgt cagtcaggct cttatcccac gcctggccaa
    40561 attcactgtg tccccagctc cactgttttt tacctggcgc aatatggtgg ttggcaacgt
    40621 gtagcaaacc accatcttca tcgtgacacc acgcgccaac aaaatcgtac tgtgattttt
    40681 cagccatata tgaggttgga acgggcacat ttttatagcg agaaatgtcc actccggcgg
    40741 agtagtccac tttgtagtaa gtgccggtgg cgatggggaa agcggagacg gcccgtttgc
    40801 cgtgatcaaa caccgccgtt acatccggcg ggaagacgct ctgatgccct tccccccctt
    40861 tcactgccgg gttggcccac cacaagaaat gacgcggcgt ggcgttgccg ttatagacgc
    40921 gactggcgat ttccagcgcc gcccggtcag ggcgcagggt gaaacctgtc atcacctgta
    40981 aaccgtgcat cgactccgtt tcgccgaccc acaccgtctg tgcgccgtcg tcatgggctt
    41041 cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg gtgttgcggc cagttaaact
    41101 caatcccgcc ggagatccac ggccccagca gtcccaccag cgccggttta atgacttcat
    41161 tgtgataaac aaaatcgcgc tgtttcactt tatcccacgc gcgatgcacc cgaccgccca
    41221 gttccggcag gatcatcact ttgatgtagt cgttttccag ccacaccgcc tgccaggatt
    41281 tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta gggataaacc gcgccggacg
    41341 atccctgata aacgcgattt tccaggaaca tgggatgtat atcctgcggc ccggtttcat
    41401 aggtcgggat ctcaacgcgc tcttgccaca cttttactgg agtcatggtg ccctcaatat
    41461 taacaagaca tactgaatta aaagatttgt ggcagtgtat tgaacaatct ggcaatgttt
    41521 tcgcggaata atcacgcaat taactaaaca aggtttagtg aagatgagag cctgcattaa
    41581 caatcaacag attcgccacc ataacaaatg cgtgattctg gaactgctgt accggcaaaa
    41641 gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt tcgattccgg cggtcagtaa
    41701 tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat atcgacgatg aaagtcaaac
    41761 gcgcggacat agtagcggta catggctgat tgcgccggaa ggtgactgga cgctgtgtct
    41821 gaacgtgacg cccaccagca ttgagtgtca ggtcgctaat gcctgtttaa gtccgaaagg
    41881 cgaatttgag tatttacaga ttgatgcacc gacgccgcag gcgctgctgt ccgaaatcga
    41941 aaaatgctgg catcgccacc gtaaattgtg gccggatcgc accatcaatc tggctctggc
    42001 aatccacggt caggtggatc cggtgaccgg cgtgtcgcaa accatgccgc aagcgccgtg
    42061 ggcaacaccg attgaggtga agtacctgct ggaagagaag ctgggtattc gggtaatggt
    42121 cgataatgac tgcgtgatgc tggcgctggc ggagaaatgg caaaataatt cgcaagtgcg
    42181 tgatttctgc gtaatcaacg ttgattacgg cattggctcg tcgttcgtga ttaacgagca
    42241 aatttatcgc ggcagtttgt atggcagtgg acagattggc cacaccatcg ttaatccgga
    42301 tggcgtcgtc tgcgactgcg ggcgttatgg ctgcctggaa accgtcgcct cgttaagcgc
    42361 attaaaaaaa caggcgcggg tatggctaaa atcacaaccg gttaatactc agcttaatcc
    42421 tgaaaaattg actaccgcgc agttaatcgc tgcctggcag agtggagaac cgtggttcac
    42481 cagttgggtt gaccacagtg ccaacgccat tggtttgagt ctgtataact tcctcaacat
    42541 cctcaatatt aatcagattt ggttgtacgg tcgcagttgt gcctttggtg agaactggct
    42601 taataccatt attcgccaga cgggatttaa cccgttcgac cgcgacgaag gaccgagcgt
    42661 gaaagcgaca cagattggct ttgggcaatt aagccgcgca caacaggtac tgggaattgg
    42721 ctatttgtat gttgaggcgc agttacggca gatttgatgg cgcgataacg tagaaaggct
    42781 tcccgaagga agcctttttt caacagatta accgtttcgc ttatgcgtaa accgggtaac
    42841 gtgcgcagat gtcgagaact ttacctttga tgcgctcgat aacggcttca tcattgatgc
    42901 tgtccagcac gtcacacatc cagccagcca gttctttcgc ttcggcttct ttaaagccac
    42961 gacgggtaat cgccggagta cctacacgaa taccggaagt cacaaatggg ctcttcggat
    43021 cgttcggtac gctgtttttg ttgacggtga tgttagcacg gcccagagcg gcgtctgctt
    43081 ctttaccggt caggttttta tcaaccagat caaccaggaa caggtggtta tcagtaccgc
    43141 cggaaaccac tttgtagccg cgttcgagga acacttctac catcgctttg gcgtttttcg
    43201 cgacctgctg ctggtaagtt ttgaactcag gttccatcgc ttctttcaga gcaaccgctt
    43261 taccggcgat tacgtgcatc aacggaccgc cctgaccacc agggaaaacg gcagagttca
    43321 gttttttgta cagctcttcg ctaccacctt tcgccaggat caggccgccg cgcggacccg
    43381 ccagggtttt gtgagtggtg gtagttacta cgtgagcatg aggaaccggg ttcgggtaga
    43441 cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac gaacaggtaa gcaccgatgc
    43501 tgtcagcgat ttcacgcatt ttcgcccagt caaccacgcc ggaatatgca gagaagccac
    43561 cgataatcat tttcggtttg tgttctttag cttgtttttc cagatcggcg tagtcgatat
    43621 gaccagtagc atcgataccg taaggaacga tgttgtacag tttaccggag aagttaaccg
    43681 gagaaccgtg agtcaggtga ccgccatgcg ccagattcat acccagaacg gtatcgcctg
    43741 gttccagcag cgcggtgtag accgcaaagt tagcctggga gccggagtgc ggctggacgt
    43801 tagcgtagtc agcgccgaac agttctttcg cacgatcgat cgccagttgt tcaacgatat
    43861 caacatactc gcaaccgccg tagtagcgtt tgcccggata accttcagca tatttgttgg
    43921 tcagctgaga accctgcgcc tgcattacgc gcgggctggt gtagttttcg gaggcgatca
    43981 gttcgatgtg ctcttcctga cgtacttttt cctgctccat agcctgccac agttcggcat
    44041 cataatcggc aatgttcatt tcacgcttta acatccgcat ctcctgactc agctaacaat
    44101 aaaatttttg gcctttatag gcggtcctgt tggacaacgg cgaacagtat aaccgaatca
    44161 ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac gattaccttc aggctacgca
    44221 aggctttgga gaataaagag cttgcaaccg gaaacggatt tcttttcagg tttgtgatgc
    44281 aaatttttca cttcatcaca ttctttctga aaaacaccaa agaaccattt acattgcagg
    44341 gctatttttt ataagatgca tttgagatac atcaattaag atgcaaaaaa aaggaagacc
    44401 atatgcttga cgctcaaacc atcgctacag taaaagccac catcccttta ctggtggaaa
    44461 cgggaccaaa gttaaccgcc catttctacg accgtatgtt tactcataac ccagaactca
    44521 aagaaatttt taacatgagt aaccagcgta atggcgatca acgtgaagcc ctgtttaacg
    44581 ctgttgccgc ctacgccagc aatattgaaa acctgcctgc gctgctgcca gcggtagaaa
    44641 aaatcgcgca gaagcacacc agtttccaga tcaaaccgga acagtacaac atcgtcggtg
    44701 aacacctgtt ggcaacgctg gacgaaatgt tcagcccggg gcaggaagtg ctggacgcgt
    44761 ggggtaaagc ctatggcgta ctggctaatg tatttatcaa tcgcgaggcg gaaatctata
    44821 acgaaaacgc cagcaaagcc ggtggctggg aaggtactcg cgatttccgc attgtggcta
    44881 aaacaccacg cagcgcactt atcaccagct tcgaactgga gccggtcgac ggtggcgcag
    44941 tggcagaata ccgtccgggg caatatctcg gcgtctggct gaagccggaa ggtttcccgc
    45001 atcaggaaat tcgtcagtac tctttgactc gcaaaccgga tggcaaaggc tatcgtattg
    45061 cggtgaaacg cgaagagggt gggcaggtat ccaactggct gcacaatcac gccaatgttg
    45121 gcgatgtcgt gaaactggtc gctccggcag gtgatttctt tatggctgtc gcagatgaca
    45181 caccagtgac gttaatctct gccggtgtcg gtcaaacgcc aatgctggca atgctcgaca
    45241 cgctggcaaa agcaggccac acagcacaag tgaactggtt ccatgcggca gaaaatggcg
    45301 atgttcacgc ctttgccgat gaagttaagg aactgggtca atcactgcca cgctttaccg
    45361 cacacacctg gtatcgtcag ccgagcgaag ccgatcgcgc taaaggtcag tttgatagcg
    45421 aaggtctgat ggatttgagc aaactggaag gtgcgttcag cgatccgaca atgcagttct
    45481 atctctgcgg cccggttggc ttcatgcagt ttgccgcgaa acagttagtg gatttgggcg
    45541 tgaaacagga aaacattcat tacgaatgct ttggcccgca taaggtgctg tgatttgatg
    45601 ttgccggatg gacacatccg gcaacccttg acgcgtttta aattgccgcg tcgtcctctt
    45661 caccggtacg gatgcgaatg acccgtgcca cgtcgaagac gaagatttta ccatcaccga
    45721 ttttgccggt ttgcgccgtg cgaataatgg tatcgacaca ggtatcgaca atgtcgtccg
    45781 gtacgacaat ctcaattttc actttcggca gaaaatccac catatactcc gcaccacggt
    45841 acagctcggt atggcctttc tggcggccaa aacctttcac ctcagtgacc gtcatgccgg
    45901 taataccgac ttcagccagt gcttcgcgga catcgtccag cttgaagggt ttaataatcg
    45961 catcaatctt tttcatgcta ttccttgaaa aggtcgcctg tcttttgatc tgctaaacgt
    46021 aacacataac gccaattcat tccttgaaat cgtttgcatc cagctcgttt cgggaaagca
    46081 gtttataaaa ttctgtccgg ttgcgccccg ccattctcgc cgcgtgggtg acgttgcctt
    46141 tggtgatttg cagcagctta cgcaaatagt tgagttcaaa ctgattacgt gcctcaacaa
    46201 aggttggcag cgccgtattt tcaccctcca gcgcctgctc caccagcgca tcactaatta
    46261 ccggagatga ggtcagcgcc acacactgtt caatcacgtt gaccaactgg cgcacgttgc
    46321 ccggccagct tgcagtcatc aggcgtttca ttgcatcggt ggagaacgcg cggacaaacg
    46381 gtttatgtcg ctctgccgcc tggcgcaaca ggtgatttgc cagtagcgga atgtcttctg
    46441 tgcgctccgc cagtgccgga attttcaggc tgacaacatt gaggcggtaa taaagatctt
    46501 cacggaattc cccacgcgcc atcgcttttg gcaggtcgcg gtgagtggcg gaaataatcc
    46561 gcacgtcaat atcaatatcg cggttactgc ccagcgggcg cactttacgc tcctgcaaca
    46621 cgcgcagcag tttgacctgc aacggtgcag gcatatcgcc aatctcatca agaaatagcg
    46681 taccgccttc cgccgcctgg aataaacctt cgcgattgct gacagcgcca gtaaacgcgc
    46741 cacgtgcatg accaaacagc tccgactcca gcaattgctc gggtaatgcg ccacagttaa
    46801 tagcaataaa tggcttgctg ttgcgcgggc tggcgttgtg gatagcctgg gcgaaaatct
    46861 ctttcccggt gccgctctgg ccgttaatca aaacgctaac gtctgattgt gccaccagcc
    46921 gcgcctgttc cagcaagcgc agcatcagcg ggctgcgggt gacaatcgcc tcgcgccaac
    46981 gttcgtcggt ggctggcgcg gattgttcca gcgcatcgtc aattgcctga tatagcgcat
    47041 ctttatcgac aggcttggtg agaaaactga acacgccctg ctgtgttgca gcaacggcat
    47101 caggaataga accatgcgcg gtaagaataa ttactggcat tcccggctgc actttctgga
    47161 tttcagcaaa cagctgcata ccgtccattt catccatccg caggtcgctg atgactaaat
    47221 ctactttttc gcgattcagt acccgcaatc cttcagcacc actttccgcc gtgaccacac
    47281 tgtagccttc gctggtcaga cgcaggccaa gcagtttcag caatcccgga tcgtcatcga
    47341 ccagtaataa atgcgcaggt ttatggctca tcaggagtga cctcatcggt ggatggcgcg
    47401 ggcttttcac tctcgtgcgg cgtatccggc gagaaatttc cagccggttt gcgggtcgag
    47461 agctggcgtt caatatcggt caggttttcc agcttgcgag tagtgagttc cagttgctgt
    47521 tgtagaacgt gatgttgctg gcgcaaagta tccagctcgc tgtcgctgga ctgctggagt
    47581 ttgctgtagc gttggcgctc ttccgccagt tgcagttgca gcgcctgacc gtcgcgccaa
    47641 agttgataca gtgggcgaac ctgtgccggg atctcggtac ttagcgcatc aatgcgcgcg
    47701 accagctggc ggcgctcata cggcgtaatt ttggcgtcgg cgagcaaaat cccttgttta
    47761 aaggtatttt gccagttgcc gtcgtcatat tggcgggctt gctgacgcga ctgcgcaggc
    47821 attaaacgat cagcacaatc catcgcccgc agccagtaaa gcggattggt ttcggttgat
    47881 ttaccttgca gcgcccagat gtcgctacat tcagtagaaa gatagtcagc cagttgataa
    47941 accggaattt tttcttctgc tggcgtatca atggctggcc tattgtgact ctgcacgcaa
    48001 cccagcaatg ccagacatgg cagccctgcc agccacagcc gtcggggcaa taatcgttga
    48061 aaaatgtgtc gcatattcac cagacttaaa gcctatccca gtgggcgtaa ttgttgcagg
    48121 cagtttggac atggacagcg cggagaaacc ggagcgtaca tgtcgtacgt gaagatttcg
    48181 agcactgccc ggggccgaac tgacaaataa aatagcctga tgggatgggc tgttagcatt
    48241 atttcgtgtt tttcgacgac ggtaattcaa tgcggaaaca aacgtcttgc ccgctctcgt
    48301 cgaccagata cagttcccct tgcatacggc gaatacaatc cctggcaatg cttaatccca
    48361 gaccgctgcc cttcaccgcc ccttttcgct ggtggcttcc ctgaaaaaag ggttcgaaga
    48421 tcatggcgcg ttcctcttgc ggaatgggcg tgcctgtatt gatgacatca atataaaccc
    48481 gcgcaccatg taaactgctg cgaaggcaaa tgttaccgga ttcagccccg tagtgcaccg
    48541 cattggagta aagattatcc agtacgctca tcagcagcat tggctccgcc aggcaagctg
    48601 ttgctttgag atcaacgtcg gtatgcatca ttttagcccg tgcgggcagg ctatgagcag
    48661 aaaccaatgt ctccaccagc ggtgctaact caacattctc cagttccacc gcactgtccg
    48721 cctgtttacg gttgtaatca agcagttgtt cgatcagttt ttgcaaattg cggctgctgc
    48781 tatcaagaat gctcaccacc tctttttgct ctggcgtaag cggcccgaca acctgatcag
    48841 ccagtaattc agtgccctcg cgcatactcg ccagtggcgt ttttaattca tgagataaat
    48901 gtcttaaaaa ttgatggcgt tgggattcca gccatgacag gcgctcactt aaccaaagaa
    48961 tacgttgccc aaccgagcgt aactcgctcg gtccactgaa cgagacgcta ttgcccagag
    49021 aacgcccttc ccccagccgg ttgatcatgc gctcgatatt tttcaccgga ccgataatca
    49081 tcctcgtgaa aagcagtacc atcaccagac tcaccagaaa tagcaccagc gattgccaac
    49141 caaaatattg cccacgttcg gcgatttcac gctgaagttg ctgcccacga gagaacacca
    49201 ctgtgcgcgt ggcctgcacc atttcggtat tggcactggc aaaggcttcc agacgcgcgg
    49261 cggcagcagc atcgggaccg ctgttgttac actgaagttg agccagattg ttcaaatcct
    49321 gacgtaatgc ctggtagagt ttatcgtccg gcagcacgcc tgcgtgggca tcgagcattt
    49381 cgctgtaacg cttgcgctgg ctttgataaa ccttcgcaag cgtagggtcg tccagcacgc
    49441 agtactgacg gtagctgcgc tccatctcca gtgccgcgtt ggtcatcgct tcactgcgcc
    49501 gggcatcaat aagcgtagtg cggttaacca gcgccgcctg atcgctaagt gcattcaggc
    49561 tttgccaggc ttgccatgcc agcaccaaca ggggcagcag aatcagcaaa aatgccagca
    49621 ttaccagttg tcgtaatgag cggggaaaaa cgggccagcg tttcaaggcg ttactctcgt
    49681 cagacgcgaa tagcctgatg ctaaccgagg ggaagttcag atacaacaaa gccgggaatt
    49741 acccggcttt gttatggaat aaggcggtgc ctaactcgac gtttcgcccg atggttgata
    49801 tagctaagct gatatcagaa gttggacggc aggcaccttg ttgtgcgtca ttcgaatttt
    49861 atgtagcacg tcccgaaggg gctgacataa gtcggtgaat gagccactgc ttactattat
    49921 gcagtaactg tgccaataaa gaaaatagtt tggtaacata ctgattatac gctgctttga
    49981 ggggtttatg gcttctccgc tatttgaatg taaatcagcc actgtgtcgc taaaaagaga
    50041 caacttaaga taaacttatt agataatatt aaaatcaatg agttaagtgt caccaaaaag
    50101 cgacacggca aaccacccat tgtcgtgatt taaagacaca aaaaagctcc cgaagttggg
    50161 agctcataat agcggttggt gcttaatgcc ggatgcggcg tgaatgcctc atccgggctg
    50221 caataccaat gggttgacga cttaccccaa ctgcttacgt gcattgcgga aaatgcgcat
    50281 ccatgggcca tcctcgcccc agttttccgg atgccaggag ttgctgacag tacggaaaac
    50341 tcgttccggg tgcggcatca taatggtgac tcgaccactt tcagtcgtga ccgccgtaat
    50401 accgttcggt gaaccgttcg ggttagccgg gtaggtttca gtgactttgc cgaagttatc
    50461 gacatagcgc agtgccacca gccctttgct ttccagtgcc gccagatgcg ccgcatcacg
    50521 cacttccacg cgcccttcac catgagacac agcaatcggc atctgcgagc ccaccatccc
    50581 ctgcaacagc agcgacgggc tttgggttac ttctaccaga ctgaaacgcg cttcaaagcg
    50641 atcggaggtg ttacgcacaa aacgtggcca caactcgcta cccgggatca gttcacgcag
    50701 attagacatc atctggcaac cgttacatac ccccagcgcc agcgtttgcg gacgatggaa
    50761 gaaggttgca aactcatcgc gtacacggtc attgaacagg atagacttcg cccaaccttc
    50821 accagcgccc agcacatcac cgtaggagaa accaccacac gcgaccaggg cgtggaaacc
    50881 ctccaggccc gtgcgtccag ccagcagatc gctcatatgc acgtcgatag catcgaagcc
    50941 tgcacggtgg aaagctgccg ccatttcaac atgcgagttc acgccctgct cacgcagcac
    51001 ggcaactttc gggcgcacgc cagtggcaat aaacggtgct gccacatctt cgttgatatc
    51061 aaacgacagt ttcacattca ggcccggatc ggcgtcgttt gatttcgcct ggtgttcctg
    51121 atcggcacac tccgggttgt cacgcaggcg ctgcatctgc caggtagttt ctgcccacca
    51181 gacacgcagc gtggtgcggc tttcgctaaa tacagtctgc ccgttggcag taatcacaaa
    51241 acggtcaccg gaaaccgcct gcccaacata atggacgcaa tcagcaagcc catgctgtgc
    51301 cagtaccgct tcaacggctt cacgatcagc agctcgaacc tgaatcaccg cacccagttc
    51361 ttcgttaaac aacgccgcca ggcgatcgtc acccagagag gcgatatccg cgtcaatgcc
    51421 acaatgacca gcaaaggcca tttccgccag cgttaccagc aggccgccat cagagcggtc
    51481 gtgatacgcc agcagcttac gctgtgcaac cagcgcctga atcgcgtcat agaagccttt
    51541 cagttgggca acatcgcgta catctgccgg tttgtcgcca agctgacgat aaacctgcgc
    51601 cagtgccgtt gcgcccagcg cgttattgcc tttgcccaaa tcaatcagca acagtgcgtt
    51661 atcttcggta gaaagctgcg gcgtaatggt gtgacgtaca tcttccacgc gggcaaatgc
    51721 ggaaatcacc agcgacaacg gcgacgtcat ttcgcgctct tcgttacctt cctgccagcg
    51781 ggttttcatc gacatagagt ctttacccac cggtatcgtc aggcccagcg ccggacaaag
    51841 ttcttcgccc acagctttaa cggcttcata caggcccgca tcttcaccag ggtggcctgc
    51901 cgccgccatc cagttggcgg aaagtttaat gcgtttgata tcgccaattt gtgttgcggc
    51961 gatgttggtt agcgcttcac cgactgccag acgggcagaa gcggcgaaat ccagcagcgc
    52021 aactggcgca cgttcgccaa ttgccatcgc ttcgccatag tagctgtcga ggctggcggt
    52081 agtgaccgcg cagttagcga ccggcacctg ccacggcccc accatctgat cgcgcgccac
    52141 catgccggtt acgctgcggt caccgatggt caccaggaaa gttttctccg ccacggtcgg
    52201 cagatgcagc acgcgtttca ccgcgtcagc gatggtgatc ccttcacggg ccagcgcgtc
    52261 gcctttcgct ttcagcgttt gcacatcgcg ggtcatcttc ggcgttttgc caagcagtac
    52321 gtccagcggt agatcaatcg gctgattatc aaaatgacga tcgtgcagag aaagatgcag
    52381 ttcttcggtc gcttcaccaa tcaccgcgta cggtgcgcgt tcacgcttac acagttcgtc
    52441 aaacagcggt aactgatcgg cagcaactgc cagcacgtag cgttcctggg attcgttaca
    52501 ccagatttcc agcgggctca tgcctggctc gtcgctcaga atatcgcgca gttcgaattt
    52561 accgccgcgc ccgccgtcgc tcaccagttc cggcatggcg ttagaaagac cgcctgcgcc
    52621 aacgtcgtgg ataaacagga ttgggttggc atcaccaagc tgccagcaac ggtcgatcac
    52681 ttcctggcag cgacgctcca tctccgggtt gtcgcgctgt acggaagcaa agtcgaggtc
    52741 ggcatcagac tgaccagatg ccatggaaga cgccgcgccg ccgccaagac cgatgttcat
    52801 tgccgggccg ccgagaacga ccagcttcgc accaacgttg atctcgtctt tttgtacgtg
    52861 atcggcgcga atgttgccga tcccacccgc cagcatgatc ggtttgtgat aaccgcgcag
    52921 ctcttcgcca ttgtggctgt tcactttttc ttcataagta cggaagtagc cgttcagtgc
    52981 cgggcgacca aattcgttgt taaacgccgc gccgcccagc gggccttcgg tcatgatgtc
    53041 cagcgcggtg acaatgcgct caggcttacc gaaatcttct tcccacggct gttcaaagcc
    53101 cggaatacgc aagttggata cggagaaacc aaccagaccg gctttcggct ttgcgccgcg
    53161 cccggtggca ccttcatcgc ggatttcacc gccagaaccg gtcgccgcac ccggccacgg
    53221 agaaatcgcc gtcgggtggt tgtgggtttc aactttcatc agaatatgcg ccggttcctg
    53281 atggaaatcg tagcggcccg tttcgtggtc agcaaagtag cgacccactt cagaaccttc
    53341 cattacagcg gcgttatctt tataagcaga gagaacgtgg tctggcgtgg tttcgaaggt
    53401 gtttttgatc atcttgaaca gcgatttcgg ctgctgttca ccgtcgataa tccagtcggc
    53461 gttaaaaatt ttgtgacggc agtgctcgga gttcgcctgc gcgaacatat acagctcgat
    53521 gtcgttcggg ttacgaccca gctttgtgaa cgcatcctgc agatagtcaa tttcatcttc
    53581 cgccagtgcc aggccaagac gcaggttagc gtcgattagc gcctgacggc cctgccccag
    53641 caaatctacg ctggtgaccg gagtcggttg atggtgtgca aacaactgct ctgcatcatc
    53701 taaagcaaaa aagaccgttt ccatcatgcg gtcgtgcagt tcagcggtca cctgctgcca
    53761 ttgttcattg gtcagcgtac cggcttctat atagtaagcc atgccgcgct caaggcggtt
    53821 tacctgttgt agaccgcaat tatgggcgat atcggtcgct ttcgaagacc agggagagat
    53881 ggtgccagga cgcggggtca ccagcaggag tttgccttgc ggggcgtggc tggcgagtgc
    53941 cgggccatat ttcagcaggc gttcaagttg tgcgtgctca tcatcgttta acggcgcatt
    54001 gaggtcagca aaatggacat actcggcgta aatagtgtga accgggagcc tggcagcctg
    54061 aaaacgtgcc agcagtttgt tgattcggaa tgccgacaat gcaggcgaac cacgcagaat
    54121 ttccatcata agtctctcgt cttctaagct ttcggtgtac ccaagggggg gaaacgggcg
    54181 tcattataaa gaatctgatg cgctgacgaa accgtttgcg tggaaataaa atcaccatcg
    54241 tgaattagca acgcgtgccg ccaatggctg taataagttg ccatctggcg caggtttacg
    54301 caaaatgccg ctcatttatg agtaaacctt tcactattat tacgtttttt caagctggga
    54361 cgcgcacgac acagagaatt aactaattga aaaaattaaa gattaattat ctgttcatcg
    54421 gcattctggc actgctgctc gcggtcgctc tctggccatc cattccctgg tttggtaaag
    54481 ccgacaaccg tatcgccgcc attcaagcgc ggggagagtt gcgtgtgagc accattcata
    54541 ctcccctgac ttataacgaa atcaacggga aaccttttgg cctggattac gaactggcga
    54601 aacagtttgc cgattacctc ggtgtaaaac tgaaagtgac cgtgcggcag aatatcagcc
    54661 agctgtttga cgacctcgat aatggtaacg ccgacctgct ggcggcagga ctggtctata
    54721 acagtgagcg ggtaaaaaat tatcagcctg gtcctaccta ttattccgtg tcacaacaac
    54781 tggtttataa agtgggtcag tatcgcccac gtacgctggg caacctgacg gcggagcaac
    54841 tcaccgttgc accgggtcac gtggtggtta acgatctcca gaccctgaaa gacacaaaat
    54901 tcccggaatt aagctggaag gtagacgaca aaaaaggctc tgcggaatta atggaagatg
    54961 tcatcgaagg aaaactcgat tacaccattg ctgattctgt cgccatcagc ctgtttcagc
    55021 gcgttcaccc ggaactcgcc gtagcgctcg atatcaccga tgaacaaccg gtgacctggt
    55081 ttagcccgtt agatggcgat aatacccttt ccgccgccct gctcgacttc ttcaacgaaa
    55141 tgaatgaaga cggtacactg gcacgcattg aagagaaata cctggggcat ggcgatgatt
    55201 ttgattacgt cgatacgcgc acatttttac gcgccgtcga tgcggtactg ccgcagttaa
    55261 agcccctgtt tgagaaatac gccgaagaaa ttgactggcg tttgctggcc gctattgctt
    55321 atcaggaatc gcactgggat gcgcaggcca cttcaccgac gggtgtgcgc ggcatgatga
    55381 tgttaaccaa aaacaccgcg caaagcctcg gcattacgga tcgtaccgat gccgaacaga
    55441 gcatcagcgg cggcgtgcgt tatttacagg atatgatgag taaagtgccg gaaagtgtgc
    55501 cggagaacga acggatctgg tttgccctcg ccgcgtacaa tatgggctat gcgcatatgc
    55561 tggatgcccg cgccctgacg gcaaaaacca aaggtaatcc tgacagttgg gctgacgtaa
    55621 aacagcgtct gcctttactt agccagaaac cctattacag caagctgact tacggctacg
    55681 ctcgtgggca tgaagcctac gcttatgtcg aaaatattcg taaataccag attagcctgg
    55741 tgggttatct gcaggagaaa gagaagcagg ctacagaagc ggcgatgcaa ctggcgcagg
    55801 attatccggc ggtatcgcct acggagctgg gcaaagagaa atttcctttt ctctcgtttc
    55861 tttcccagtc gtcatcaaac tatttgaccc actctccctc tctgctgttt tccagaaaag
    55921 ggagtgaaga gaaacaaaat taatctgtcg aggattgcgc ttttttctgc gctttaattt
    55981 cctggcggcg catgcgaaag aagtcactga gcaacgccgc acactcatcc gccagtattc
    56041 cttccgtaat ttccacccgg tgattcatac ccggatgatg cagcacatcc attaaagatc
    56101 ccgcagcgcc agttttcgcg tcacgcgcac caaagaccac gcgaccaatg cgactgtgga
    56161 tcattgctcc ggcacacatt acacagggtt caagcgtgac atacaacgtg gcgtcgatca
    56221 gacgataatt ttgcatcacc agaccgccct gccgtagggc catgatttct gcatgtgcgg
    56281 tgggatcatg tcgaccaatc ggacggttcc agccttcgcc aataacacga ttgttatgca
    56341 ctaataccgc gccgaccggt acttcccgct catcccaggc acgcttcgcc agcgtcattg
    56401 catgacgcat ccagtattcg tagctaaatt cgacttcaga caaaaagaaa actccggtta
    56461 taaaagcgcg gcgcattata cacggacgct atgctttact ctattccagt tgctggagtt
    56521 caccgcgcgg ggttacccgc cagcgatgct ggcaaaagta aagcagcggg ttgtcctggt
    56581 tactgtcgct atagccactg tacagccgca gcggcgtgcc gattttgcgc tccagttgcg
    56641 cgaccttctc atgcccgagg caacgcatcg tcaataccca accaccatag ccacgctgaa
    56701 tctgactggc gataagatta acccgcggca gccagggcgt atcgaaataa accgcttcaa
    56761 ccagcggctg cggagagccg gtaatcaacc agatatcagc atcggaactt aacaggtagg
    56821 tggttaatcg ctcctgaacc agcggaaagg cggtaacatt gtcacgaaac cagcgtacga
    56881 aatcggcctg caacgcctgc aaacgcgctt cattgtgacc aaaagtgcat ccccacagaa
    56941 gcagactcat cggccagcgt gccgcacgac cttttatcaa taacgcaatg gctatgatcg
    57001 gcaacaacgg cagaacaagt aacgcattca gcggttggcg acgcagtaaa taacgcaaaa
    57061 aactgccgaa catatcctgc tgatgcaatg ttccatctaa gtcaaaaaac accacacgac
    57121 gctcgtgagt tgccaaacca tactcctctg gactgaaaca tcctgattaa ttttcgctac
    57181 tacatagcct aacagataga tcatcacttt tccggcaaca atcgaccgca tcatggctaa
    57241 ctggaatttt taattcatgg caattagcgg caatggaata taaaattcac tcgcgtgtgt
    57301 ctcatattgc ctgttgtcgc cattatttca tcgcgaggaa agatatgaac ggcttacttc
    57361 gtatccgtca gcgttaccag gggcttgccc agagtgataa aaaactggcg gattatctgc
    57421 tgctacaacc tgatacggcg cgccatttaa gctctcagca actggccaac gaagccggag
    57481 tcagtcagtc cagcgtcgtg aagttcgcgc aaaaactcgg ctataaaggt tttccggcac
    57541 taaagttggc gttgagtgaa gcgctggcaa gccagccgga atctccctcc gtgcccattc
    57601 ataaccaaat ccgcggtgat gatccgttac ggctggtcgg cgaaaaactg attaaagaaa
    57661 atactgccgc tatgtacgca acgttaaacg ttaatagtga agagaaactg catgaatgcg
    57721 tggcaatgtt gcgctctgcg cggcggataa ttctgaccgg tattggcgct tcgggtctgg
    57781 tggcgcaaaa ctttgcctgg aagctgatga agattggctt caatgctgcc gcagtgcgcg
    57841 atatgcatgc gctactcgct accgtacagg cgtcgtcccc tgacgatctg ttattagcca
    57901 tttcctacac cggtgtacgg cgcgagttaa acctggcggc agatgagatg ctgcgagtgg
    57961 gcggaaaagt gctggcgatt accggcttta ctcccaatgc cctgcaacag cgtgcttctc
    58021 attgcctgta taccattgcc gaagaacaag cgacaaacag tgcttcaatc tctgcttgtc
    58081 acgcgcaggg aatgttaacg gatttgctgt tcattgcgct gattcagcag gatctggaac
    58141 tggcaccaga acgtattcgt catagcgaag cgctggtgaa aaaactggtc tgagtaaaga
    58201 atgcgcgtat aatgcccgcc cggtttgtgt tgttttgaga gtttccttat ggcgctgtta
    58261 atcactaaaa aatgcatcaa ttgtgatatg tgtgaacccg aatgcccgaa tgaggcgatt
    58321 tcgatgggtg aacatatcta cgagattaac agcgataagt gtaccgaatg cgtagggcac
    58381 tacgagacac caacctgcca gaaggtgtgt ccgatcccca atactattgt gaaagatccg
    58441 caacatgtcg agacggaaga acagttgtgg gataaatttg tgctgatgca ccacgcggat
    58501 a
//
LOCUS       DACADF010000008        56357 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.008, whole genome shotgun sequence.
ACCESSION   DACADF010000008 DACADF010000000
VERSION     DACADF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.008"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            99..326
                     /gene="feoA"
                     /locus_tag="ISB30_000501"
     CDS             99..326
                     /gene="feoA"
                     /locus_tag="ISB30_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAP1988180.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            343..2664
                     /gene="feoB"
                     /locus_tag="ISB30_000502"
     CDS             343..2664
                     /gene="feoB"
                     /locus_tag="ISB30_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAP1988181.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQIASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2675..2911
                     /gene="feoC"
                     /locus_tag="ISB30_000503"
     CDS             2675..2911
                     /gene="feoC"
                     /locus_tag="ISB30_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAP1988182.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLKQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLHEWWALR"
     gene            3130..3540
                     /locus_tag="ISB30_000504"
     CDS             3130..3540
                     /locus_tag="ISB30_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1988183.1"
                     /translation="MRCSNSFYANRRRHASMALLELIQKHIRQRDLIGLVEQMACLLS
                     SGYANDRQIKGLFNYILQTGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSK
                     ALHIAKIMLESGVPLADIMRFTGVSEEELAAASQ"
     gene            complement(3569..4339)
                     /gene="bioH"
                     /locus_tag="ISB30_000505"
     CDS             complement(3569..4339)
                     /gene="bioH"
                     /locus_tag="ISB30_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAP1988184.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPDEFCHLLVALKQRV"
     gene            4377..5060
                     /gene="gntX"
                     /locus_tag="ISB30_000506"
     CDS             4377..5060
                     /gene="gntX"
                     /locus_tag="ISB30_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAP1988185.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLLTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5119..5694
                     /gene="nfuA"
                     /locus_tag="ISB30_000507"
     CDS             5119..5694
                     /gene="nfuA"
                     /locus_tag="ISB30_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAP1988186.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6058..7374
                     /gene="gntT"
                     /locus_tag="ISB30_000508"
     CDS             6058..7374
                     /gene="gntT"
                     /locus_tag="ISB30_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAP1988187.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(7484..9568)
                     /gene="malQ"
                     /locus_tag="ISB30_000509"
     CDS             complement(7484..9568)
                     /gene="malQ"
                     /locus_tag="ISB30_000509"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAP1988188.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVIVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYLANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(9578..11971)
                     /gene="malP"
                     /locus_tag="ISB30_000510"
     CDS             complement(9578..11971)
                     /gene="malP"
                     /locus_tag="ISB30_000510"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAP1988189.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWVKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            12594..15299
                     /gene="malT"
                     /locus_tag="ISB30_000511"
     CDS             12594..15299
                     /gene="malT"
                     /locus_tag="ISB30_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HAP1988190.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(15342..15824)
                     /locus_tag="ISB30_000512"
     CDS             complement(15342..15824)
                     /locus_tag="ISB30_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal subunit interface protein"
                     /protein_id="HAP1988191.1"
                     /translation="MMRPLVAKVAIRVTLAESSRKLPRHVAEREIATLAGSFSLHEQN
                     IHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLASPAAV
                     GEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTRVSIE
                     "
     gene            15733..16128
                     /locus_tag="ISB30_000513"
     CDS             15733..16128
                     /locus_tag="ISB30_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1988192.1"
                     /translation="MSRSATWRGNFRELSASVTRMATFATSGRITLDTVEDEINRLRY
                     NWQESRPSTLTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRELFDISRQGKA
                     SVNDADRLRKYLARFGLTWEAMQDQHSSS"
     gene            complement(16110..16868)
                     /locus_tag="ISB30_000514"
     CDS             complement(16110..16868)
                     /locus_tag="ISB30_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAP1988193.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTMPPASVMQVLKDHHIQLELC"
     gene            complement(16885..17715)
                     /gene="glpG"
                     /locus_tag="ISB30_000515"
     CDS             complement(16885..17715)
                     /gene="glpG"
                     /locus_tag="ISB30_000515"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAP1988194.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQSGHTDSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(17760..18086)
                     /gene="glpE"
                     /locus_tag="ISB30_000516"
     CDS             complement(17760..18086)
                     /gene="glpE"
                     /locus_tag="ISB30_000516"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAP1988195.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHATQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18276..19781
                     /gene="glpD"
                     /locus_tag="ISB30_000517"
     CDS             18276..19781
                     /gene="glpD"
                     /locus_tag="ISB30_000517"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAP1988196.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLMEYTQQRLSLAS"
     gene            complement(19835..20440)
                     /locus_tag="ISB30_000518"
     CDS             complement(19835..20440)
                     /locus_tag="ISB30_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988197.1"
                     /translation="MNKIRLYIYPVIFGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELLVEYALFFSVMGGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSHRKKWGDWVTCLSLAILALGPLFNYLQ"
     gene            complement(20443..21210)
                     /locus_tag="ISB30_000519"
     CDS             complement(20443..21210)
                     /locus_tag="ISB30_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988198.1"
                     /translation="MHPTPAMQALIEQIYHIFRRYPAPQQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPCLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEREILQQFACQYMTDWVSGDEAVELQYKLEMFFRADIALSPLLDAIIS
                     VPGFWSAASLACLLNTYRDGYIRDNQDDIDKAITTQTNTWASNNQSILKERARQAIEN
                     PLKQSEQGTQYQAWEDEWMIDECLCAMYDASSESSGH"
     gene            complement(21313..22821)
                     /locus_tag="ISB30_000520"
     CDS             complement(21313..22821)
                     /locus_tag="ISB30_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP1988199.1"
                     /translation="MMQSGKWILTSLVMTFFGIPILAQFLAAVVAMLGAGLAAILEVC
                     NLLFTPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPEKREIAAWRQCFFLLPALLTL
                     VGWIIALHLADYQFRQMGSGWLANLMLSWQGVLLLSLISGDYWWIVIIPVGAHISFSL
                     GYGWPTRHPLTGTSGLRCRNLLLFLLLLLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHVWDYLD
                     NSRTQEAYNKIVNGDADIIFVAQPSDGQKKRAEKSGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRAVGGDDQEIQTWQRPEDSGSQTVMLSQVMKNVRMISP
                     QETEVASVMEGMINVVAEYRNTKNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNDKYPYIVDALMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPMDKTLH"
     gene            complement(22946..25393)
                     /gene="glgP"
                     /locus_tag="ISB30_000521"
     CDS             complement(22946..25393)
                     /gene="glgP"
                     /locus_tag="ISB30_000521"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAP1988200.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADYIWHIDPVRL"
     gene            complement(25412..26845)
                     /gene="glgA"
                     /locus_tag="ISB30_000522"
     CDS             complement(25412..26845)
                     /gene="glgA"
                     /locus_tag="ISB30_000522"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAP1988201.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(26845..28140)
                     /gene="glgC"
                     /locus_tag="ISB30_000523"
     CDS             complement(26845..28140)
                     /gene="glgC"
                     /locus_tag="ISB30_000523"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAP1988202.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPGKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(28158..30131)
                     /gene="glgX"
                     /locus_tag="ISB30_000524"
     CDS             complement(28158..30131)
                     /gene="glgX"
                     /locus_tag="ISB30_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAP1988203.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVIDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNDRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(30128..32314)
                     /gene="glgB"
                     /locus_tag="ISB30_000525"
     CDS             complement(30128..32314)
                     /gene="glgB"
                     /locus_tag="ISB30_000525"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAP1988204.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(32587..33690)
                     /gene="asd"
                     /locus_tag="ISB30_000526"
     CDS             complement(32587..33690)
                     /gene="asd"
                     /locus_tag="ISB30_000526"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP1988205.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            33882..34475
                     /gene="yhgN"
                     /locus_tag="ISB30_000527"
     CDS             33882..34475
                     /gene="yhgN"
                     /locus_tag="ISB30_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAP1988206.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(34521..35816)
                     /locus_tag="ISB30_000528"
     CDS             complement(34521..35816)
                     /locus_tag="ISB30_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HAP1988207.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEVGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPMQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(35723..37819)
                     /locus_tag="ISB30_000529"
     CDS             complement(35723..37819)
                     /locus_tag="ISB30_000529"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HAP1988208.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGGVDCLLISPERLANQDFLETVLYPVADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPILGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSDLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(37960..39300)
                     /gene="gntU"
                     /locus_tag="ISB30_000530"
     CDS             complement(37960..39300)
                     /gene="gntU"
                     /locus_tag="ISB30_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAP1988209.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRVH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(39304..39831)
                     /gene="gntK"
                     /locus_tag="ISB30_000531"
     CDS             complement(39304..39831)
                     /gene="gntK"
                     /locus_tag="ISB30_000531"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP1988210.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(39970..40965)
                     /locus_tag="ISB30_000532"
     CDS             complement(39970..40965)
                     /locus_tag="ISB30_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GntR"
                     /protein_id="HAP1988211.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(41058..41753)
                     /locus_tag="ISB30_000533"
     CDS             complement(41058..41753)
                     /locus_tag="ISB30_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAP1988212.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRIWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEILLFDLPPV"
     gene            complement(41876..42913)
                     /locus_tag="ISB30_000534"
     CDS             complement(41876..42913)
                     /locus_tag="ISB30_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP1988213.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSAVSLAR"
     gene            43247..43735
                     /gene="yhhY"
                     /locus_tag="ISB30_000535"
     CDS             43247..43735
                     /gene="yhhY"
                     /locus_tag="ISB30_000535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1988214.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(43796..45529)
                     /gene="ggt"
                     /locus_tag="ISB30_000536"
     CDS             complement(43796..45529)
                     /gene="ggt"
                     /locus_tag="ISB30_000536"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAP1988215.1"
                     /translation="MIKPTFLRRVAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDVTATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNTDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLISKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPSVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            45649..46089
                     /locus_tag="ISB30_000537"
     CDS             45649..46089
                     /locus_tag="ISB30_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAP1988216.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(46076..46819)
                     /gene="ugpQ"
                     /locus_tag="ISB30_000538"
     CDS             complement(46076..46819)
                     /gene="ugpQ"
                     /locus_tag="ISB30_000538"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP1988217.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCRKHGMMANIEIKPTTDTGPLTGKMVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNYKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(46816..47886)
                     /locus_tag="ISB30_000539"
     CDS             complement(46816..47886)
                     /locus_tag="ISB30_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HAP1988218.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGIALPLNGGYRQYVGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGHWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(47888..48733)
                     /gene="ugpE"
                     /locus_tag="ISB30_000540"
     CDS             complement(47888..48733)
                     /gene="ugpE"
                     /locus_tag="ISB30_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAP1988219.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(48730..49617)
                     /gene="ugpA"
                     /locus_tag="ISB30_000541"
     CDS             complement(48730..49617)
                     /gene="ugpA"
                     /locus_tag="ISB30_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAP1988220.1"
                     /translation="MPSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(49715..51031)
                     /gene="ugpB"
                     /locus_tag="ISB30_000542"
     CDS             complement(49715..51031)
                     /gene="ugpB"
                     /locus_tag="ISB30_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAP1988221.1"
                     /translation="MKPLRYTASALALGLALMANAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEARIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(51428..52141)
                     /gene="livF"
                     /locus_tag="ISB30_000543"
     CDS             complement(51428..52141)
                     /gene="livF"
                     /locus_tag="ISB30_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAP1988222.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(52143..52910)
                     /gene="livG"
                     /locus_tag="ISB30_000544"
     CDS             complement(52143..52910)
                     /gene="livG"
                     /locus_tag="ISB30_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAP1988223.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGMPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(52907..54184)
                     /locus_tag="ISB30_000545"
     CDS             complement(52907..54184)
                     /locus_tag="ISB30_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAP1988224.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(54181..55107)
                     /gene="livH"
                     /locus_tag="ISB30_000546"
     CDS             complement(54181..55107)
                     /gene="livH"
                     /locus_tag="ISB30_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAP1988225.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(55155..56264)
                     /gene="livk"
                     /locus_tag="ISB30_000547"
     CDS             complement(55155..56264)
                     /gene="livk"
                     /locus_tag="ISB30_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAP1988226.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT        13626 a        15432 c        14071 g        13228 t
ORIGIN      
        1 atttaatctc tccttcgttg gcaaatcatc tggtctcatg tcgctgtcaa acgccccatg
       61 aggtagttat ccagttaatg agaaacaagt aggcacctat gcaatacact ccagatactg
      121 cgtggaaaat tactggcttt tcccgtgaaa tcagcccggc atatcgccaa aaactgcttt
      181 ctcttggcat gttacctggc tcctctttta atgtggtgcg cgtcgctcca ctcggcgacc
      241 ccattcatat cgaaacccgt cgtgtgagcc tggtattacg caaaaaagat ctggccttat
      301 tagaagtgga agcggtttcc tgttaatacg gtgataacaa caatgaaaaa attaaccatt
      361 ggcttaattg gtaatccaaa ttctggcaag acaacgttat ttaaccagct tactggctca
      421 cgtcagcgtg taggtaactg ggctggcgtt accgtcgaac gtaaagaagg gcaattctcc
      481 accaccgatc atcaggtcac gctggtggac ctgcccggca cctattctct gaccaccatc
      541 tcatcgcaga cctcgctcga tgagcaaatc gcctgtcact acattttgag tggcgacgca
      601 gacctgctga ttaacgtggt ggatgcgtct aaccttgagc gtaacctgta cctgacgctg
      661 caactgttgg aactcggcat tccctgcatc gtggcgctga acatgctcga cattgccgag
      721 aagcaaaata ttcgtattga aattgatgct ctgtcggcgc gtcttggctg cccggtgatc
      781 ccgctggttt caacccgtgg tcgcggtatt gaagcgctca agctggcgat tgatcgctat
      841 aaagctaacg agaatgtgga actggtgcat tacgctcagc cgctgctcaa cgaagcagat
      901 tcactggcaa aagtgatgcc ttccgacatc ccgctgaaac aacgtcgctg gctgggcctg
      961 caaatgctgg aaggcgatat ctacagccgc gcctacgccg gtgaagcgtc gcagcatctg
     1021 gatgccgccc tcgcccgtct gcgtaatgag atggacgatc cggcgctgca cattgccgat
     1081 gcgcgttacc agtgcattgc tgccatctgt gatgtggtaa gcaacaccct gacggcagaa
     1141 cccagccgtt tcaccactgc ggtagataaa atcgtgctca accgtttcct cggtctgccg
     1201 attttcctct ttgtgatgta cctgatgttc ttgctggcta tcaacatcgg cggggcgtta
     1261 cagccgctgt ttgacgtcgg ctccgtagca ctctttgtgc atggtattca gtggattggc
     1321 tacacgctcc acttcccgga ctggctgact atcttcctcg cccaaggcct gggcggcggt
     1381 attaacaccg tgctgccact ggtgccgcag atcggcatga tgtacctgtt cctctccttc
     1441 cttgaggact ccggctatat ggcgcgtgcg gcgtttgtga tggaccgtct gatgcaggcg
     1501 ctgggcttgc cggggaaatc ctttgtaccg ctgatcgttg gtttcggttg taacgtaccg
     1561 tcggtaatgg gcgcacgtac gcttgatgca ccgcgtgaac gtctgatgac catcatgatg
     1621 gcaccgttta tgtcctgcgg cgcgcgtctg gctatcttcg cagtattcgc ggctgccttc
     1681 ttcgggcaga acggtgcgct ggcggtcttc tcgctgtata tgctgggtat cgtaatggcg
     1741 gtgctgactg gcctgatgct caagtacacc atcatgcgcg gtgaagcgac gccgtttgtc
     1801 atggagctgc cggtctacca tgtaccccac gttaaaagcc tgattatcca gacctggcag
     1861 cgtctgaaag gcttcgttct gcgtgctggt aaagtgatta tcatcgtcag cattttcctg
     1921 agcgccttca acagcttctc attgagcggg aaaatcgtcg ataacatcaa cgactcggcg
     1981 ctggcttccg tcagccgggt gatcaccccg gtcttcaagc cgattggcgt acatgaagat
     2041 aactggcagg caacggttgg cctgtttaca ggtgccatgg cgaaagaagt ggtggtgggt
     2101 acgctcaaca ccctctacac cgcagaaaat attcaggacg aagagttcaa tccggcggaa
     2161 tttaacctcg gtgaagagct gttcagtgcg gtagatgaaa cctggcagag cctgaaagac
     2221 accttcagcc ttagcgtact gatgaacccc attgaagcaa gcaaaggcga cggcgaaatg
     2281 ggtaccggag cgatgggcgt gatggatcag aaattcggta gcgcagctgc cgcatacagc
     2341 tacctgattt tcgtcctgct gtatgtacca tgtatctcgg tgatgggagc catcgcccgt
     2401 gaatcaagcc gtggctggat gggcttctcc attctgtggg ggctgaatat cgcttactcg
     2461 ctggcaacat tgttctatca gatcgccagc tacagccagc atccaaccta cagcctggta
     2521 tgcattctgg cggttattct gtttaacatc gtggttatcg gtttgctgcg tcgcgcgcgt
     2581 agccgggtgg atatcgaact gctggcaacg cgcaaatcgg taagcagttg ctgtgcggcc
     2641 agtaccactg gcgattgcca ctaaggacac gacaatggct tcgctcattc aagtgcgtga
     2701 tttactggca ttacggggcc gtatggaagc ggcccagatt agccagacac tgaacactcc
     2761 gcagccaatg attaacgcca tgctgaaaca gctagaaagt atgggaaaag cagtgcggat
     2821 tcaggaagaa cctgacggct gtctttccgg cagttgtaaa agctgcccgg aaggaaaagc
     2881 ctgtctgcac gaatggtggg cgttgcgtta accttgtttc actgcctgtt ttgaaagccc
     2941 ggtatgcgtt tgcatccggg cttttttgcg tgcggctttc catagcagcg cgacgtaagt
     3001 tcctctgaaa gcgtctcgct gggatgaaaa ctcgctaatg cacaggtgtg gagtggcgcg
     3061 tagagtcgcg gcattcaaat aacaggtgaa ggaacgccat gagcaaaaag cagagttcca
     3121 ccccacacga tgcgctgttc aaactctttt tacgccaacc ggagacggca cgcgagcatg
     3181 gcgctgctgg agttaattca aaaacatatt cgtcaacgcg atctgatagg actggtagag
     3241 caaatggcct gcttattaag tagtggatac gcaaatgaca gacaaatcaa agggctgttt
     3301 aattacatac tgcaaaccgg cgacgcggta cgttttaacg attttatcga tggtgtggcc
     3361 gaacgttcac caaaacacaa ggagagttta atgactattg cggaaagatt gcggcaggaa
     3421 ggggaacaat ccaaagcctt gcatatagca aaaataatgc ttgaatccgg agtccctctt
     3481 gcagacatca tgcgctttac cggggtgtca gaagaagagt tggctgcggc gagccagtaa
     3541 agttctgtcc cgccatttcg aaagccacct acaccctctg cttcaacgcc accagcaggt
     3601 gacaaaactc gtccggatgt gaaataaacg gcgcatgggc cgctttggcg aagatatatg
     3661 attcgctgtg aggccaaagt ttatccagca tcggcaccac tttgcgcggc accagaccgt
     3721 cgagatagcc atacaatcgc aaaaacggca tgggcacgtt ttgcagcggc tgacggagat
     3781 cgaccgtttt caggatttcc agtccgccat tgagtacgtc aacctccggc atcggtaacg
     3841 ccagaacggt tttcttcaac gcccgcgcat cctggcgcgc cgtttcagta cccatggttt
     3901 gtaacgccag gaaccgctcc actgtacgct gaaaatcatc actgagttgc tgctgaaatc
     3961 ccgccagcac gtccggtttt atccccggcc actcgtcgcg agcactaaaa caaggtgacg
     4021 acgccacggt gaccagcgcc tgaacacgct cgggatgggt taacgcaatc tggcttgcca
     4081 ccagcccgcc cagactccag cctaaccaaa tggctttatc tggtgcctgt cgcagcacga
     4141 cttcggccat atcagcaagt gatagcgcac caaatccccg gctacgcccg aagccgggca
     4201 ggtcaacaag gtgcagcgta aaatgcgagc taagttcctc gtcaatgcaa cgccacactt
     4261 cggcattcag tccccatccg tgcagcagca caagatgaac attcccctga cctttggtct
     4321 gccaccagat gttattcatc cgctattgtt ctctttttac ttacaaggat gaacatatgc
     4381 taacagtacc gggattatgc tggctatgcc gaatgccgct ggcgttaggt cattggggga
     4441 tttgttctgt ctgctctcgc gccgcccgta cagataaaac gttatgccca caatgtggat
     4501 tacccgccac acactctcat cttccctgcg gtcgctgcct gcaaaaaccg ctgccctggc
     4561 aaagactgct cacggtcgcc gactatgcgc cgccattaag tccgctcatt caccagctta
     4621 agttttcccg gcgcagtgaa atcgccagcg ccctgtcacg tctgttacta ttggaagtct
     4681 tacacgctcg tcgcaccacc ggcttgcaac tgcctgatcg catcatcagc gttccgttat
     4741 ggcagcgccg tcactggcgt cggggattta atcagagcga tttgctgtgt cagccgttat
     4801 cacgctggct acactgccaa tgggatagcg aagccgtcac acgaacacgg gccaccgcga
     4861 cccagcattt tcttaacgcc cggctacgca aacacaacct gaaaaatgcc tttcgccttg
     4921 aattgcctgt gcaaggtcgc catatggtga ttgtggatga tgtcgttacc accggaagta
     4981 ccgtcgcaga gattgcgcag ttgcttttac gcaatggtgc ggcgactgtc caggtctggt
     5041 gcctttgtcg aaccttgtag agcctcgatg atgggcgtat tataaccaac taaaatagtc
     5101 aactattagg ccattactat gatccgtatt tccgatgctg cacaagcgca ctttgccaaa
     5161 ctgctggcaa accaggaaga agggacacaa atccgcgtat ttgtgattaa ccctggcacg
     5221 cctaacgctg aatgtggcgt ttcttattgt ccgccggacg ctgtggaagc caccgacact
     5281 gccctgaaat ttgacctgct gaccgcgtat gttgatgagt taagcgctcc ttacctggaa
     5341 gatgcagaga tcgattttgt taccgaccag ttgggttcgc agctgacgct gaaagcgcct
     5401 aacgccaaaa tgcgtaaagt ggctgacgat gcgccgctga tggagcgcgt ggagtatatg
     5461 ctgcaatcac agatcaaccc acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa
     5521 atcaccgaag acggttacgc cattttgcaa tttggcggtg gctgtaacgg ttgttccatg
     5581 gtcgacgtca ccctgaaaga ggggatcgag aagcagttgc tgaacgaatt cccggagctg
     5641 aaaggtgtac gcgatctcac cgaacaccag cgcggcgagc actcttacta ctaagttctc
     5701 tccccttttg tcgattgccc gatgtgacgc taaagcgtct tatcgggcct gtacgttgca
     5761 cgagagtctg acgttagcgc agaattatct cagcaaatcc aatctcttcg tttccccacg
     5821 ataatatgac caacctctca taatttaaat ttaccccgct ctggtgattc tcaagcacgt
     5881 aatgttaccc gtatcattct catgggcacc aaacacactc ataacatctg actacactaa
     5941 ctcgttttag gggtcatcag ccgtggtgcc gtaacatctc tgttcccagt tgggacaacc
     6001 aggattttcc gtcagaaacc tgacgttacc cataacaaat taaaggccag gtaaatcatg
     6061 ccattagtca ttgttgctat cggtgtaatc ttgttattac tcctgatgat ccgcttcaaa
     6121 atgaacggct tcatcgctct cgtcctcgtg gcgcttgctg ttggattaat gcaagggatg
     6181 ccgctggata aagttattgg ctccatcaaa gctggtgtcg gcggaacgct cggtagcctt
     6241 gccctgatca tgggttttgg tgcgatgctg ggcaaaatgc tggcagactg cggtggtgca
     6301 caacgtatcg ctaccacgct gattgccaaa tttggtaaaa aacacatcca gtgggcggtg
     6361 gtactgaccg gttttaccgt tggttttgcc ctgttctatg aagtgggttt cgtgctgatg
     6421 ctgccgctgg tgtttaccat cgcggcttcc gcgaatattc cactgctgta tgttggtgta
     6481 ccaatggcgg ctgcactgtc tgtgacccac ggcttcctgc caccgcatcc gggtccgact
     6541 gcgattgcca ccattttcaa tgccgatatg ggtaaaactc tgctgtacgg tactattctg
     6601 gcaatcccga ccgtgattct cgccggtccg gtttacgctc gcgtgttgaa aggtatcgat
     6661 aagccgatcc cggaaggtct gtacagcgcg aaaaccttca gcgaagaaga gatgccgagc
     6721 tttggcgtca gcgtctggac ctctctggtg ccggtggttc tgatggcgat gcgtgcgatt
     6781 gccgaaatga tcctgccgaa aggtcacgct ttcctgccgg tagcggagtt cctcggtgac
     6841 ccggtaatgg ctacgctgat tgccgtgctg atcgcgatgt tcacctttgg tctgaaccgt
     6901 ggccgttcaa tggatcagat taacgacacg ctggtttctt ccatcaaaat cattgcgatg
     6961 atgctgttga ttatcggtgg tggcggtgcg ttcaagcagg tgctggtaga cagcggcgtg
     7021 gacaaataca ttgcttccat gatgcacgaa accaacattt ctccgctgct gatggcctgg
     7081 tcgattgctg ccgtactgcg tatcgcgctg ggttccgcaa ccgtcgcggc aatcactgcg
     7141 ggtggtatcg cggcaccgct gattgcaacg acgggtgtta gcccggagct gatggttatt
     7201 gcggttggtt ccggtagtgt gattttctct cacgtgaacg atccgggctt ctggctgttc
     7261 aaagagtact ttaacctgac tatcggcgag accatcaaat cctggtcgat gctggaaacc
     7321 attatctcgg tgtgcggtct ggtaggctgt ctgctgctga atatggtgat ttgataatac
     7381 tgatggccgg atgcggcgtg aacgccttat ccggcctacg attcggcatt aattagtagg
     7441 ccggtaagac gcgtcaagcg tcgcatccgg catcaaccgc actctacttc ttcttcgctg
     7501 ccgctctgcg ccgtctgtcc aaatccttca gcaacttgtt cacaccatca tcggcaaaca
     7561 tcgactcaag cgttgcggaa agtttacgtc gccagttttt atactggtaa ctggtgccgg
     7621 gaatatttac cggatctgcc atatccagcc agtcttccgg ctggagccct aacagggcgc
     7681 tgttgctatc ggcgatgtag cgctgtaagc cacgattcag cgtcggtgtc atcgacatca
     7741 acgatgcctt atgcccggca cgttttggca ggcaaccata tttatgcaga gcatccagca
     7801 gcccttgctt cgccagttcg cgatcctgat acagaccgcg cagtaccact tcatccggat
     7861 acagccccag cgttttgccc agcgttaaat ccccactctc ccagtagccg cgtagcgtag
     7921 gcaggtcgtg tgtcgccgca accgccatcg actgctctgg gtacgctttc ggtgcgcgga
     7981 atgtcttctc gtggtcgttt tcgaaataga gcactttgta ggagtacaca ccgctgctgc
     8041 gcagcttacc gacaatctct accggtacgg taccgagatc ttcaccaatc accatacagc
     8101 gatgacgttt actttcgagt gccagaatcg agagcagatc atccaccgga tagtgaacat
     8161 acgcgccctg atctgccgtc tcgccatacg gtatccacca caaacgcagc atcgacatca
     8221 cgtgatcaat tcgtaatgcg ccgcagtttt gcatattggc acgcaacagc tcgataaacg
     8281 gttcataggc acgcgcggtg atgatatgcg ggtccattgg cggcaatccc cagttctgcc
     8341 ccaacgggcc gaggatatcc ggcggcgcgc caaccgacgc tttcaggcaa tacagttcac
     8401 ggtcgcacca ggtttctgcc ccaccttctg ctacaccaac ggccagatca cgatacaagc
     8461 caatcggcat ttcatagccc tggcttatct cccagcaggc ggcaaactgg ctgtaagcca
     8521 gccactgcaa ccagagataa aaatcgacgt catcacgatg ttcttcgcag aactggcgca
     8581 cttctggcga atccacgttc tgatacatct ctggccatgc tggccagccc cagcgcattt
     8641 cgtcctcttt cacttgctgg gcatgtagcg catcaaaggc tgcctgccag aacaggctgt
     8701 cgccctgctc tgcaacaaac tggcgaaacg cggtcatctg ctcatcatca cgttgcgcga
     8761 aacctttcca cgccattcgt aatgctgtca tttttagggc ggtaaccgtg gagtaatcaa
     8821 cccagtcggc atcccgcgcc tgttgcagcg tctgttgcgt ggtcggcaac tgccaccagg
     8881 cctgagcctc ttcgctaaga tggaaatctt caactgcgtt aacgtcgata taaatcacat
     8941 tcagccaacg gcgagaagac gggctgtacg ggctggcact ttccggattt gccagataga
     9001 gcgcatgaat cgggttcaga ccaataaacg acccgccacg ttttgccaca tccaccagca
     9061 tcgctttgag atcgccaaaa tccccaatac cccagttttt ttccgatcgc agcgtataaa
     9121 gctgaacgca ggcaccccac agcttttgtt tattcagcaa cgcctgcggt tcgtaacagc
     9181 gtttcggggc gacaatcacc cggcaatgcg cgcgctggtc gtcctgggta agcgtcagcg
     9241 tgtgataacc ttccggcagc ttcgtcggta gattgaacgc tttgcccccc gtcacatggc
     9301 ctttgtactg ctttccttct tcggtggtca gcagccagct atattcgccg ctgccctcca
     9361 ccaccatcgg catttttttg ccgctggtat aaactatgac attcgggact ggcgttaccg
     9421 ccactttcgt ggcggtacgt tgatgcatcg cgtcaagcaa acgccgtttg gtttcggcgc
     9481 taatcgactg cggtttaccg tgggcattga tgtaattggg gctaatcccc gccgccagcg
     9541 cggcattatc cagacgtttg ctttccatcg aacttcctta gcgttttgcc tgccagatac
     9601 gagcctgata atcgcgaata gagcgatccg agctaaacat accgcagcgg gcggtattga
     9661 ggatcgccgc gcgagtccag gcctcctggt cgcggtacag cacatccacc tgcttttgtg
     9721 cctccacata agcggcgaaa tctgccatca ccagatacgg atcgccgccc tgtttgccga
     9781 tactgtgcag catctggtcg aaggcatgct tatcgccgtc gctgtattta ccgctttcca
     9841 gctctttcaa tactgcgtcc agcaccttat ctttcttccg ccatttcacc gggtcgtagc
     9901 ctttggccag aattgccttc acttgttcca cggtatgacc aaaaataaag atattttctt
     9961 cgccgacttt ctcagcgatt tcaacgttcg caccatccag cgtaccgaca gtaagcgcac
    10021 cattgagcgc cagtttcata ttgccggtac cggaagcttc tttacctgca gtcgaaattt
    10081 gttcggagat atccgccgcc gggatcagtt tttccgccgc cgaaacacaa taatccggca
    10141 agaacaccac cttcaacttg tcgccaacca gcggatcgtt gttgatcacg tcagccactt
    10201 tgttgatcgc aaagataata tttttcgcca ggtagtagcc cggtgctgct ttcgcgccga
    10261 agaggaagac gcgcggtacg cggtcagact gcgggttttc acgaatttct ttgtacaacg
    10321 ccagaatatg cagcagattc agatgctggc gtttgtactc atgcagacgt ttgatctgaa
    10381 tatcgaaaat cgcctgtgga ttgatctcaa taccggtacg aactttcaca aactctgcca
    10441 gacggacttt attcgcctgc ttgatctcgc gatattgctg acggaatttc gcatcatcag
    10501 cgaatttttc cagattgatc agttgatcga gatcgttagc ccactctttt ttcagtgatt
    10561 tatccaacag agccgccagt gccgggttgc actgtttgac ccagcgacgt ggggtaatac
    10621 cgttggtgac attatggaat ttgttcggcc atagctggtg atattccggg aacagatctt
    10681 tcaccaccag atccgagtgc agcgccgcaa caccgttcac cgcgaaaccg ccaaccacac
    10741 acaggttcgc catatgcact tgtttgtcgt gcaccaccgc cagtttggcc cacacttttt
    10801 catcgcccgg ccaggttttc tccactagcg ttttaaagcg agtattaatt tcgttaataa
    10861 tctgcatgtg gcgcggcagt aagcctttca ccagtttcac atcccagcgt tccagcgctt
    10921 ctggcatcag ggtatggttg gtgtaagcga aagttttgct ggtaatggcc caggcgtcat
    10981 cccagctcat ctggtgctca tcgatcagca cgcgcagcag ttctggaatc gcgattgttg
    11041 gatgggtatc gttcagctga ataacttcgt aatccgccag ttcgtgcaat ttacgccccg
    11101 ccagatgatg gcgacgcaaa atatccgcta ccgagcaggc acactggaag tattgctgca
    11161 tcaggcgcag ctttttaccg gcagtatggt tgtcgtttgg atagagaact ttggtcagtt
    11221 tttctgcgtt gatgccctgc tgttcggcac gcaggaaatc accatcgtta aatttagtca
    11281 gatcaaacgg atgcgcatgc gtcgcctgcc acagacgcag cggctgcgcc acgccattac
    11341 gatagccgac aacggggaga tcccacgctt gaccggtaat ggtaaattcc ggctcccagc
    11401 gtccgtcttt cgtcacttta ccgccaatcc ctacctgcac atccagtgct tcgttgtggc
    11461 ggaaccacgg gtagttaccg cgatgccagt catccggcgc ttcaacctgt ttgccatcga
    11521 caaaagactg gcggaacaaa ccatattgat agttcagacc gtaacccgtc gcagactgac
    11581 cgacagttgc cattgagtcg agaaagcacg ccgccagacg tcccagacca ccgttcccca
    11641 gcgccgggtc gatctcttct tccaacaggt cagtcaggtt gatgtcataa gccttcaacg
    11701 aatcctgtac atcctgatac cagccgagat tcaacagatt gttgcccgtc aggcgaccaa
    11761 tcaaaaactc cattgagatg tagttaacat gccgctgatt cgccaccggt ttggcaaatg
    11821 gctgagcacg cagcatttcg gccagtgctt cgctcactgc cagccaccac tgacgagggg
    11881 tcatttcagc cgcagaattt aagccataac gctgccactg acgtgaaagc gcttcctgaa
    11941 attgcttatc gttaaaaata ggttgtgaca taggagttcc acttttctta gattttcaac
    12001 acaacgttat cgctagtttg ccaggctcga tgttgacctt cctcatcctg cgggggatta
    12061 ggcagggagg agttgcgggg atgagcaagg aaatgtgatc tcgaccactt aaagctagtg
    12121 caatccacag gattagcagc aaatcaatgc cataccgcgc agaaaatctg tatctaagtg
    12181 caaaaaatgg ccgttgcgta ttttcaaaaa gcggaaggta actctataaa ttaagtaaag
    12241 gagtgaaaca gtttcacaag taaaatatca cgtgtgctcc atctcattct taatagattt
    12301 attaagatca tctttttaga tggcactttc atcagaaatg aagaagaaac ccttgcttaa
    12361 atgaaactga tgaacataag gaaaataccg tacaacccag tattcacgct ggatcagcgt
    12421 cgttttaggt gagttgttaa taaacatttg gaattgtgac acagtgcaaa ttcagacaca
    12481 taaaaaaacg tcatcgcttg cattagaaag gtttctggcc gaccttataa ccattaatta
    12541 cgaagcgcaa aaaaaataat atttcctcat tttccacagt gaagtgatta actatgctga
    12601 ttccgtcaaa attaagtcgc ccggttcgac tcgaccatac cgtggttcgt gagcgcctgc
    12661 tggctaaact ttccggcgcg aacaacttcc ggctggcgct gatcacaagt cctgcgggct
    12721 acggaaagac cacgctcatt tcccagtggg cggcaggcaa aaacgatatc ggctggtact
    12781 cgctggatga aggtgataac cagcaagagc gtttcgccag ctatctcatt gccgccgtgc
    12841 aacaggcaac caacggtcac tgcgcgatat gtgagacgat ggcgcaaaaa cggcaatatg
    12901 ccagcctgac gtcactcttc gcccagcttt tcattgagct ggcggaatgg catagcccac
    12961 tttatctggt catcgatgac tatcatctga tcactaatcc tgtgatccac gagtcaatgc
    13021 gcttctttat tcgccatcaa ccagaaaatc tcacccttgt ggtgttgtca cgcaaccttc
    13081 cgcaactggg cattgccaat ctgcgtgttc gtgaccagct gctggaaatt ggcagtcagc
    13141 aactggcatt tacccatcag gaagcgaaac agttttttga ttgccgtctg tcatcgccga
    13201 ttgaagctgc agaaagcagt cggatttgtg atgatgtttc cggttgggcg acggcactgc
    13261 agctaatcgc cctctccgcc cggcagaata ctcactcagc ccataagtcg gcacgccgtc
    13321 tggcgggaat caatgccagc catctttcgg attatctggt cgatgaggtt ttagataacg
    13381 tcgatctcgc aacacgccat tttctgttga aaagcgccat tttgcgctca atgaacgatg
    13441 cactcatcac ccgtgtgacc ggcgaagaaa acgggcaaat gcgcctcgaa gagattgagc
    13501 gtcaggggct gtttttacag cggatggatg ataccggcga gtggttttgc tatcacccac
    13561 tgtttggtaa cttcctgcgc cagcgctgcc agtgggaact ggcggcggag ctgccggaaa
    13621 tccaccgtgc cgccgcagaa agctggatgg cccagggatt tcccagcgaa gcgattcatc
    13681 atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat tctgcttaat cacgcctgga
    13741 gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc gcttaaggcc ctgccgtggg
    13801 acagtttgct ggaaaatccg cagttggtgt tattgcaggc gtggctgatg caaagccagc
    13861 atcgctacgg cgaagttaac accctgctgg cccgcgctga acatgaaatc aaggacatca
    13921 gagaaggcac catgcacgca gaatttaacg ctctgcgcgc ccaggtggcg attaacgatg
    13981 gtaatccgga tgaagcggaa cggctggcaa aactggcact ggaagagctg ccgccgggct
    14041 ggttctatag ccgcattgtg gcaacctcgg tgctgggtga agtgttgcac tgcaaaggcg
    14101 aattgacccg ctcactggcg ttaatgcagc aaaccgaaca gatggcacgc cagcacgatg
    14161 tctggcacta cgctttgtgg agtttaatcc agcaaagtga aattctgttt gcccaagggt
    14221 tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca gctgatcaac gagcagcatc
    14281 tggaacagct gccaatgcat gagtttctgg tgcgcattcg tgcgcaactg ttatgggcct
    14341 gggcacggct ggatgaagcc gaagcgtcgg cgcgcagcgg gattgaagtc ttgtcgtctt
    14401 atcagccaca gcaacagctt cagtgcctgg caatgttgat tcaatgctcg ctggcccgtg
    14461 gtgatttaga taacgcccgt agccagctga accgtctgga aaacctgctg gggaatggca
    14521 aatatcacag cgactggatc tctaacgcca acaaagtccg ggtgatttac tggcaaatga
    14581 ccagcgataa agccgccgct gccaactggt tgcgtcatac ggctaaacca gagtttgcga
    14641 acaaccactt cctgcaaggt caatggcgca acattgcccg tgcacaaatc ttgctgggcg
    14701 agtttgaatc tgcggaaatt gtcctcgaag aactcaatga aaatgcccgc agtctgcggt
    14761 tgatgagcga tctcaaccgt aacctgttgc tgcttaatca actgtactgg caggccggac
    14821 gtaaaagtga cgctcagcgt gtgttgctgg acgcattaaa actggcgaat cgtaccggat
    14881 ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca acaactgcgc cagcttattc
    14941 aactcaatac gctgccggag ctggaacagc atcgcgcaca gcgtattctg cgagaaatca
    15001 atcaacatca tcggcataaa ttcgcccatt tcgatgagaa tttcgttgaa cgtctgctaa
    15061 atcatccgga agtgccagag ctgatccgca ccagcccgtt gacccaacgt gaatggcagg
    15121 tcctggggct gatttactcc ggttacagca atgagcaaat tgcaggcgag ctggaagtgg
    15181 cggcaaccac catcaaaacg catatccgta atctgtacca aaaactcggt gtcgcccacc
    15241 gtcaggccgc ggtacaacac gcgcagaaac tgctgaagat gatgggatat ggggtgtgag
    15301 tttagccgga taatgcgtcg gatccggctt acatctttac atcattcaat gctaacccgc
    15361 gttacgccat ctgtttctat caaaccaaac cgcaccggca agaaacgctc caccacggcg
    15421 atattggtca gcagatgaca tgagggatgg gcgaccgtaa attctcccgc gcccgccagc
    15481 gccatcggta gcaccagttg gtcggcgaga tattccccca ccgccgccgg gcttgccagg
    15541 tagcgtttca cctctttcac caactgtgcc gcgaccacct cggcactgac gcgcttttca
    15601 ccgacgacaa aaaagcgttc ggtgatattt tcactttcga cttcaagcga gacggtatta
    15661 cccggcccct ggtcgcgcgg caggttatga atattctgtt catgcaagga aaaactaccc
    15721 gccagtgtag cgatttcacg ctcagcaaca tggcgcggta actttcgcga actttctgcc
    15781 agcgtcacgc ggatggccac ctttgccact agcggacgca tcacactgga tacagtggaa
    15841 gatgagatca accgtctgcg ctataactgg caggagagtc gcccctccac gcttacggcg
    15901 ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc gtatgcaact ggaacacgtt
    15961 atcgctatct gccgccaggc aaagtcgctt tctgctgccg gacgtgaact gtttgacatt
    16021 tcgcgccagg gtaaagccag cgtcaatgac gcggatcggc tacgcaaata cctggcgcgt
    16081 tttggtctga catgggaagc aatgcaggat cagcacagct ccagttgaat atgatggtcc
    16141 ttcagcacct gcatcacgct cgcaggcggc atcgtgtcgg tgtagacagc atcgaccatg
    16201 ctgatgctac ccatattgac cattgcgtta cggccaaatt ttgagtggtc gacaaccagc
    16261 ataacgtggc gcgagttctc aatgatggcg cgtttggtgc gaacttcgtg gtaatcgaac
    16321 tccagtagcg agccgtcgct atcgatgccg cttatcccca gaatgccgaa atcaaggcgg
    16381 aactgggaga taaaatcgag cgttgcttcg ccaatgatcc cgccatcgcg gctgcgtaat
    16441 tcgccaccgg caagaatgat acggaaatcc tcttttacca tcagcgtgtt agcgacgttg
    16501 agattgttgg tcacaatgcg taaattgctg tgattaagca gcgcgtgcgc caccgcttcc
    16561 ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg gaatttgctc cgccactttg
    16621 cgggcgatgc gctctttttc ttcggtctgg gtggccttgc gatcgtgcca cggcgtgtta
    16681 accgaactgg aaggcagcgc cgcaccgcca tgatggcgca ggatcaggtt ttgctctgcc
    16741 agctcgttga gatcgcggcg aatagtctgc gggctaacgg agaaatgctc taccagctct
    16801 tcggtgctga cataaccctg ctgtttaacc agttcgataa taccgttgtg acgttgtgtt
    16861 tgtttcattg ataaatccct ggaattattt tcgttttcgc gcattgagcg aatcaacaaa
    16921 agccatcgct aaacccacgg ctaacccggc gatatgtgct ccgttcgcca tcgacatccc
    16981 aaacaaatca aaccatccgg cgacaatcca gatcaacgca aagataatta acccacgttg
    17041 caggtaaatg ccactttgcg gatcgcgttc gccacgtagc cagacgtagc ccatcagcgc
    17101 atacaccacg ccagaaagcc cgccaaacca cggcccgctg aatttttgct gcacatagcc
    17161 gcttaacagg gcgctaatga gagtaatgac aattagctta ccgctaccga ggcgtttttc
    17221 caccgcaccg ccgagatacc accaccagag caggttaaag aggatatgca tcagcgagaa
    17281 gtgcattaac gcgtgggtga agtaacgcca gaactcaaat ttcagcgtcg gatcgaatgg
    17341 ccaggccagc cataacatca cttcctgatc gccgagaatt tgcatggcga taaacaccac
    17401 cacgcaggcg atcatcatca cccaggttac cggacctgcg cgttcacgca aggcggcaaa
    17461 gaaaggataa cggcgataat gcaggccact gtcggtatga ccggactgcc aactggcggc
    17521 gagataacgt ggatctgccg ggttttcgag aaaacgagcc agctccgccc gcacgcgttc
    17581 ggcctgggac tcatccgcca gccagacatc gctttggtta tgttgttgaa tcgtgaggat
    17641 aacaccctgt gtcgccatgt aatcaacaaa cgcctgcgcc actcgggggt tagcaaaaga
    17701 ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa aaggggacag tataaagcgt
    17761 tacgcgccgt acgccacctc tgcgggaaac tggcgctgcc aggcttcaaa accgccatca
    17821 atgctgtaga ccacatcgta gccctgttgc agcagatact gcgccgcgcc tttgctgcta
    17881 ttgccgtgat aacacatcac catcaccggt gtgtcaaagt cgttatcacg cataaacgcg
    17941 cccagtgtgt cgttagttaa atggaaagcc tgggtcgcat gccccattgc gaaactctgt
    18001 ggatcgcgaa tatcgaccag caccgcctct ttttcctgca acttctggtg cgcgtcggca
    18061 acgttaatac attcgaactg atccatgcgt ctctctttct ttacaaacaa gtgggcaaat
    18121 ttaccgcaca gtttacgtcg aagcggcaga taaacgccat aatgttatac atatcactct
    18181 aaaatgtttt ttcaatgtta cccaaagcgc gattctttgc taatatgttc gataacgaac
    18241 atttatgaac tttaacgaaa gtgaatgagg gcagcatgga aaccaaagat ctgattgtga
    18301 taggtggcgg catcaatggt gctggtatcg cggcagacgc cgctggacgc ggtttatccg
    18361 tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc ttccgccagt tcaaaactca
    18421 ttcacggtgg cctgcgctac cttgagcact atgaattccg actggtcagc gaggcgctgg
    18481 ctgaacgtga agtgctactg aaaatggccc cgcatatcgc cttcccgatg cgttttcgcc
    18541 tgccgcatcg tccgcatcta cgcccggcgt ggatgattcg cattggtctg tttatgtacg
    18601 atcatctggg caagcgcacc agcctgccgg gatcaaccgg tttgcgtttt ggcgcaaact
    18661 cagtgttaaa accggaaatt aagcgcggat tcgaatattc tgactgttgg gtagacgacg
    18721 cccgtctggt actcgccaac gcccagatgg tggtacgtaa aggcggtgaa gtgcttactc
    18781 gtactcgtgc cacgtctgct cgccgcgaaa acggcctgtg gattgtggaa gcggaagata
    18841 tcgataccgg caaaaaatat acatggcagg cgcgcggcct ggttaatgct accggcccgt
    18901 gggtgaaaca atttttcgac gacgggatgc atctgccttc gccttatggc attcgcctga
    18961 tcaaaggcag ccatattgtg gtgccgcgtg tgcataccca gaagcaagcc tacattctgc
    19021 aaaacgaaga taaacgtatt gtcttcgtga tcccgtggat ggacgagttt tccatcatcg
    19081 gcaccaccga tgtggaatac aaaggcgatc caaaagcagt gaagattgaa gagagtgaaa
    19141 tcaattacct gttgaaagtg tataacacgc actttaaaaa gcagttaagc cgggacgata
    19201 tcgtctggac ctactccggc gtgcgtccgc tatgtgatga tgagtccgac tcgccgcagg
    19261 ccattacccg tgattacacc cttgatatcc atgatgaaaa cggcaaagcc ccgctgctgt
    19321 cggtattcgg cggtaagctg accacctacc gcaaactggc ggagcacgcg ctggaaaaac
    19381 tgacgccata ttatcagggc attggtccgg cgtggacgaa agagagcgtg ctgccgggtg
    19441 gtgccattga aggtgaccgt gacgattacg ccgcccgcct gcgccgccgt tacccgttcc
    19501 tgaccgaatc gctggcacgt cactacgcgc gcacttacgg cagcaacagc gaactgttgc
    19561 tcggcaatgc aggggcgata agcgacctcg gtgaagattt cggtcatgag ttctacgaag
    19621 cggagctgaa atatctggtc gaccacgaat gggtccgccg cgccgacgat gccctgtggc
    19681 gtcgcacgaa acaaggcatg tggctaaatg cggatcaaca atctcgtgtg agtcagtggc
    19741 tgatggaata tacgcagcag aggttgtcgc tggcgtcgta aggatgatgc ccggttgcga
    19801 agtgcaaccg ggctatttga attccttaga ctattcattg taaataattg aataacggcc
    19861 ccagagctaa aatcgccaaa cttaagcacg ttacccaatc gccccacttc ttcctatggc
    19921 ttctattttt gctacgccac tggcaataat atcccaacgc aaaaccaagc tgggcactaa
    19981 tcgggccgac aaaatcagtc cacggctgcg gccaaaaagg ggaaaaattc aggggaagcc
    20041 agacaaatgt cagaaagaaa tttcggatat tttccagcaa cgctgatttc tctggagaaa
    20101 tataaagaat gaaattcgag agaattaacc agaaatagac cggagaaaaa accagccagc
    20161 aatgtagcct gttttttaat tcaggaggcg ttctgcggga gaattgccaa ccgatgccac
    20221 ccattacact gaaaaaaagt gcatattcaa ccaataactc aagttgtaat tcctgaagag
    20281 aaatacgcac aaccaggagc ggaaaggaga atattatttc agccagaaaa gttaacgtga
    20341 atataagtac ggctaaggca ataaccgtcg gccataccaa taatggtacg ataacaaaac
    20401 cgaaaattac agggtaaata taaagtctga ttttattcat ctttaatgtc ctgatgattc
    20461 ggatgatgcg tcatacatgg cacataaaca ctcatcaatc atccactcgt cttcccaggc
    20521 ctgatactgt gtgccctgtt cggactgctt aagcggattc tcaatcgcct gccgcgcgcg
    20581 ttctttcagg atggattgat tattactagc ccaagtatta gtttgagtgg taatggcttt
    20641 atcgatatcg tcctgattat ctctgatata accgtcacga tacgtattca ataaacaggc
    20701 aagagacgct gccgaccaaa atccgggaac tgaaataatg gcatcaagca atggcgaaag
    20761 agcgatatcg gcacggaaaa acatttccag cttgtactgt aattcgacag cctcatcccc
    20821 ggatacccaa tcagtcatat actggcaagc aaattgctgc aatatttccc gttcatcctc
    20881 acgccagttt tctttactgg caaggttgat acgccgtaga gaaaatactt cgtgaatatt
    20941 gtcaaattgc ccctgcgcaa caaactccag taaacaaggc agaaaataac gaacctcatc
    21001 gctgttttgc ggatcaggtc ctggactact attccaggca ttgattaagg aataaggaat
    21061 cgctcgcaac gatgtattcc gtaatgcttt ttgttcctgt tgactcagac aatattcaca
    21121 acaaaccaca aattgctgcg gcgccggata acgacgaaaa atatgatata tctgctcaat
    21181 taatgcctgc atggcaggag tggggtgcat aattaccccc tcgcccgctg atcgtgcata
    21241 aacggctgat agataaaaag ggcattcgcc gcgttcagga aaatattaat gatatccata
    21301 ttgtaatcct gcttaatgca acgttttatc catcggcaca tagcccacat cttccaccaa
    21361 actctgcccc tgcggcgtta aaaaccactc caccaatttt tgtgtctctg acgtcgtatt
    21421 ttcccgcgtt accattaagg catcgacgat atagggatat ttgtcgttgc gaatattttc
    21481 cgcagtcggt gcaataccgt taatcgctag cagttttata tttttatcgg cattcatttg
    21541 tgttgcgtaa tagcggaagg tgtagcctat tgcgtttttt gtattacggt attcggcaac
    21601 aacgttaatc attccctcca tcacgcttgc cacttccgtt tcctgcggcg agatcatgcg
    21661 gacatttttc atgacctgcg atagcatcac cgtctggctg ccagagtctt ccgggcgttg
    21721 ccaggtctgg atctcctgat cgtcaccgcc aaccgcgcgc cagttggtaa ttgcgccact
    21781 gaaaatgtca cgcacctgtt gttcagtcag ggaattaacc ggattatccg cattgacgat
    21841 gaaaacaaac gcttcacggg caaatggcgt gtacagcaaa gtgacgcccg atttctcagc
    21901 gcgttttttt tgcccatcgg aaggttgcgc cacgaaaata atatcggcat caccgttaac
    21961 gattttgtta tatgcttctt gcgtacgaga gttatccaga taatcccaaa cgtgaaaatc
    22021 ctctggtatt acacttaatg cataaaatgc agaggcataa atggggtacg ccgccgtagc
    22081 gccatcgagt cgcggccagt tttgcgtaaa ttgaatttgt ggtttaccac gcagtggcgt
    22141 cagttgatta ttaagtttat cgggtcgcca ggcccaggta tcaatatttt cacgcacacc
    22201 gacaccggga ttaagctgtt tatataaata agcctgatac ccagcgacaa aaccaagtaa
    22261 gagaagtaga aacaggagta agttacggca acgtagcccg gacgtgccgg ttaaaggatg
    22321 ccttgtcggc cagccgtatc ccagcgaaaa actgatatgc gcgccaacgg gtataatgac
    22381 tatccaccag tagtcgccac tgattaatga gagtaacaaa acaccctgcc aggaaagcat
    22441 aaggtttgcc aaccagcccg atcccatctg gcgaaattga taatctgcca gatgtagcgc
    22501 gattatccag ccaaccaggg ttaatagcgc gggtaagaga aaaaagcatt gtcgccaggc
    22561 ggctatttct cttttttctg gtgcgctatc accagcccac acgcgaccag agaaaaatat
    22621 gattatcgcc cccagcgtca gcataaagac gttgagtaga agataaattg ttggcgtaaa
    22681 gagtaaattg caaacctcaa gaatagcggc aagcccggcc cccagcatgg caacaaccgc
    22741 tgccaaaaat tgcgccagta tggggatgcc aaaaaaagtc attaccagcg aggtcaaaat
    22801 ccattttccg ctttgcatta ttctttccat tcttgctgaa tggcgaaatt atactccccg
    22861 ataaccctcg ccccctctgg acacttttcc taaataaagg cgaaaaaaac gggacctttt
    22921 ggccccgttc tgttttttag cgaacttata atctcaccgg atcaatatgc caaatataat
    22981 cggcatactc tttgatcgta cggtcagaag agaagtaacc catattggca atgttcagca
    23041 tcgcttttgc ggtccactct tcctgacgct cgtagagttc gtcaacttta tcctgacaat
    23101 cgacatagct gcgataatcc gccagtacct ggtagtgatc gccgaagttg atcagcgaat
    23161 cgaccagatc gcgatagcga cccggatctt ccggactgaa tacaccgctg ccgatttgcg
    23221 tcagcacctg atgcagctcc tcatctttct cgtagtattc acgcggtttg tagccctgac
    23281 ggcgcaattc ttccacttct tccgctgtgt taccaaagat aaagatattg tcagcaccga
    23341 catgatccag catctcgaca ttcgcaccgt ccagcgtacc gatagtcagc gcgccgttaa
    23401 gcgcaaactt catgttactg gtgccggaag cttccgtccc cgccagcgaa atctgttcag
    23461 acagatcggc cgccggaatg atcaactgcg ccaggctaac actgtagttc gggatgaaca
    23521 cgactttcag cttgtcgcca atctgcggat cgttgttgat cactttcgct acgtcattga
    23581 tcaaatgaat aatgtgcttc gccatgtaat aggccgaagc cgccttaccg ccaaaaatat
    23641 tcacgcgcgg tacccacttc gcatccgggt cggccttgat gcggttatag cgggtgatca
    23701 cgtgcaacac attcatcaat tgacgtttgt attcgtgaat acgtttgatt tgcacatcga
    23761 acagcgcttt cggatttacc accacattca gctgctgggc gatatactcc gccagacgct
    23821 ttttgttctc cagcttcgcc tgatgcaccg cgtgattaac cattgggaaa tcacagtgtt
    23881 gttgcagatc attaagcagg ctaaggtcgg tgcgccagtt gcggcccagg tgttcgtcca
    23941 acacggctga aagcgatggg ttcgctaccg ccagccagcg acgcggcgtc acaccgttgg
    24001 tgacgttggt gaaacgaccc gggaagattt tcgcaaagtc ggcaaatagc gattgcacca
    24061 tcaggttaga gtgcagttcc gatacaccgt taactttgtg gctcaccaca accgccagcc
    24121 aggccatacg cacacgacga ccattggatt catcaatgat cgacgcccgt cccagcagat
    24181 cggtatcgtt cggatactgt tcctgcaagg ttttcaggaa atagtcgttg atttcaaaga
    24241 tgatctgcag gtgacgcggc agaattttac ccagcatatc gactggccag gtttccagcg
    24301 cctcgctcat tagcgtgtgg ttggtgtagg agaagacctg acaacacact tcaaacgcgt
    24361 cgtcccagct aaattggtgc tcatcgatca gcagacgcat catctcagga atcgacagca
    24421 ccggatgggt atcgttgagg tgaatcgcaa ttttatccgc caggttatcg taggttttat
    24481 gcaactgata atggcggctt aaaatgtcct gaatggtcga ggaaaccagg aagtattcct
    24541 gacgcagtcg cagctcacgc ccggagtagg tggagtcgtc cggatacagt acgcgagata
    24601 cgttctcgga gtggttttta tcttccactg ccgcgaagta gtcgccctgg ttgaatttac
    24661 cgaggttaat ttcactactg gcttgggcac tccacaaacg caacgtgttg gtcgcgtcgg
    24721 tgtcgtaacc tgggattatc tgatcgtaag ctactcccag aatctcttcg gtttcaatcc
    24781 agcgcgtttt tttaccttcc tgctgaatgc gaccaccaaa acggacttta tagcgcgtgt
    24841 tgtggcgttt gaattcccac gggttaccgt attccagcca gtagtcaggc gactctttct
    24901 ggctaccgtt aacgatgttc tgcttgaaca taccgtagtc atagcggatg ccgtaaccgc
    24961 gccccggcaa ccctaacgtc gccagagaat cgaggaagca agccgccaga cggcccaggc
    25021 caccgttacc gaggcccggg tcattttctt catcaatcag ctcttcgaga tttaatccca
    25081 tcgcttccag tgcgccctgt acatcttcgt aaattcctag cgacaacatg gcgttggaga
    25141 gcgtacggcc aatcaaaaac tccatcgaca ggtagtaaac ctgacgagtt tcttgcgaca
    25201 actgggcacg gtttgaacgt aaccagcgct ccacgagacg atcgcgcaca gcaaataacg
    25261 ttgcgttcag ccattcatgt ttattggcga cgaccggatc ctttccaatc gtaaacatca
    25321 gcttgtaagc gatagagtgc ttaagagctt ctacgctaag cgtgggcgat gaatatgtaa
    25381 acggagcatt catataggcg tttcctgaaa actatttcaa gcgatagtaa agctcacggt
    25441 acgacttcgc cgcgacctgc cagctaaaat ccattgccat agcctgacgt tgcacaaacc
    25501 gccacagtga aggacgggac cacagtacga aagcacgtcg aatagcccgt aacagcgacc
    25561 aggcattact atcttcaaag acaaacccac tggcgacgcc atctgcaagg ttctcaagag
    25621 aacagtcaga aaccgtatca gcaagcccac cggtgcgccg cactaacggc agcgtaccgt
    25681 acttcaatcc ataaagttgc gttaagccgc acggttcaaa acggctgggc accagaatga
    25741 cgtccgcgcc gcccataatg cgatgcgaaa atgcttcgtg atagccaatc tgaacgccca
    25801 cctggccggg gtattccgct gccgccgcaa ggaaaccttc ctgcagcacc ggatcgcccg
    25861 cgccgagtag cgccagctgc ccgccctgct ccagaagacc aggtaaggct tccagcacca
    25921 ggtcgagacc tttctggctg gtcagacggc tcaccactgc aaaaagcggc actttatcgt
    25981 caaccttaag ccccattgcg atttgtaact ggcgcttatt ttccgcttta tcttccaacg
    26041 tatcgcgggt gtaacgcgag gccaacagta agtccgtctc tggactccag attttctcgt
    26101 ccacgccgtt cagtacgccg gaaagacgcc cttcacggtg acgctgttgc aacagacctt
    26161 ccataccgta ggcaaactgc ggttcggtga tctcgcgagc gtaggttggg ctgactgccg
    26221 taatgtgatc ggcatagtac agaccggcct tcaggaaaga gatttgtccg ttgaattcca
    26281 gcccatgaat attaaagaat gaccatggca attggatgtc attcatgtga tgtgcataaa
    26341 acatgccttg ataggccagg ttgtgcacag taaacaccga cttcgccgga cgcccgcgcg
    26401 ccgccagata cgcaggcgca aggcctgcat gccagtcgtg cgcatgcacc acatcaggac
    26461 gccagaatgg gtcaagcccg ctggccattt ctgccccaac ccaccccagc agcgcaaaac
    26521 gcaatacgtt gtcggtatag gcaaataagt tggtatcgtg atacgggctt cccggacgat
    26581 catagagatg cggcgcgtca atcaggtata tgccaacccc gttgtaatga ccgaacaaca
    26641 gcgtgatatg tccggcgaag gtatcacgac gggatactac ctgcgcatcg gtcacgccac
    26701 ggcgaatatc gggaaatgca ggcaacagta cgcgagcgtc aacgccgtct gcgatttgtg
    26761 ctgcgggtaa tgccccaata acatcagcca gaccgccggt tttaagcagc gggaacatct
    26821 ctgaacatac atgtaaaacc tgcattatcg ctcctgttta tgccctaact tccgtagcat
    26881 ttcgcgcgtt accagcacga tgccttcttc tgaacgatag aaacgacgtg catcttcctc
    26941 tgcgttttca ccaatcacca tgccttccgg aataacacaa gcacgatcga tgacgcagcg
    27001 acgcagacgg cacgagcgac ctacccatac ttccggtaac aatacggcgg aatcaatgtt
    27061 gcagaatgaa ttcacgcgaa cgcgcgagaa cagaacggac tgcaccacca ccgaaccgga
    27121 gatcacacaa ccgccggata ccagtgagtt aagggtcatc ccgtggctac cggagcgatc
    27181 ctgcacgaat ttcgctggcg gtaatgattc attgtaggtg cgaattggcc aattgcgatc
    27241 gtacatatcc agttccggca ccacagaggc cagatcgagg ttcgctttcc agtaagcttc
    27301 cagcgtaccc acatcgcgcc agtacggctc ggcatccggg tcggattgta cgcaagagag
    27361 cgggaacggg tgcgcatagg ccagaccggc ttcggtgatc ttgggaatca aatctttgcc
    27421 aaagtcgtgg ctggagttct catcgcgatc gtcttcttcc agcagttcat acagatagtc
    27481 ggcgtcaaag acgtagatac ccatactcgc cagagatttg cctggatcgt tcggcattga
    27541 cggcgggtta gctggttttt caacgaattc gataatttta tcgttctcat caaccgccat
    27601 aacgccaaac gcggaggctt cttcaatcgg tactggcata caagcaacgg tgcaacgtgc
    27661 gcctttttcg acgtggtcga taagcatacg cgagtagtct tgcttgtaga tatggtcgcc
    27721 cgccaggatc accacgtatt ccgctttata acggcggata atgtcgaggt tttgggtgac
    27781 cgcatctgcg gtgccgcgat accagttttc ccctttcatt ctctgctgtg ctggcagcag
    27841 atcgacaaac tcgttcattt cttcattgaa gaatgaccag ccgcgctgga tgtgctgcac
    27901 cagagtgtgg gactggtact gggtgatcac gcccatacga cggatcccgg agttgatgca
    27961 gttagacagc gcaaagtcga taatgcggaa cttaccgccg aagtgtacgg ccggttttgc
    28021 tcgcttattg gttaaatcct tcaggcgggt accacgtcct cccgccagta tcagggcaac
    28081 agatttcaat ggcagctggc gtgccaacat taagtgatcg ttcttctcta aactaaccat
    28141 gactaactcc ttttttatca tctctggaac acacacaatc cgtgtgcagg tccctgccag
    28201 acagccgtaa tcactgggtt atcctctcca gcgaatgggg gaatggcgtg ccactcccca
    28261 gcaggtaaaa caatctctgt tacctcaagc gtggcgttaa ttgcgatcaa aaagcgatcc
    28321 gagagcagaa tttgcagctg tttcggcccg ttttgccact catccgtgct taaaggttga
    28381 gcatatcgat ttagccaacg gacattgccg tcgccttctt cccaccagcg attctccatc
    28441 aaagcgggaa tgcgcttgcg cagatggatt aacgcggcgg taaatgcggt taaaccactg
    28501 cttgcctgcg accagtccaa ccaggttaat tggttatcct gacagtaggc attgttattg
    28561 ccatgctggc tgtgaccatg ttcgtcaccg gccagtaaca tcggcgtacc ctgggagagc
    28621 aacaacgttg ttaacagggc gtgaatgctg tcgcgccgcc gttcaaccag atcaagagta
    28681 ccgcctaacc cttctttacc atgattgtta ctgtaattgt tgttggtccc gtcgcgattt
    28741 tcttctccgt ttgcttcatt gtgtttatgg ttgaagcaaa cgcagtcgcg aagcgtaaaa
    28801 ccgtcatgcg cggtgacgag attaatcgcg gcactcggca gacgatcatt acgtttaaaa
    28861 acatcgctgg aggcagcaaa acgcccggca aacgccccca gaggcaaatc ataatgcagc
    28921 cagaaacgac gggctgcatc gcggaaatga tcgttccact cggcaaacag cggcgggaaa
    28981 tttcccacct gataaccacc aggagcgata tcccacggtt cagcaattaa cttcacctgc
    29041 gagagcaccg ggcagttctg gatagcggta aacaacggcg catcctgacg aaactctggc
    29101 gtacggccca tgactgccgc cagatcaaag cggaaaccat cgacgtggca ggtttctacc
    29161 caataacgca ggcaggcgct ggcataatct accaccgccg gatgactcaa attgagcgtg
    29221 ttgccacaac cggtccagtt gtgataatcg ccgtcttctc ttatccaata atagctacgg
    29281 ttgtcgatcc cacgcagcga gaataacggg ccgtcgaggt ccagttccgc actatggttg
    29341 agcacgatat caagaatgac ttcgataccc gctttatgca gtgctttgat tgcatcgcga
    29401 aactcatcca gcgccgtttc tggcgagcag gcatacgccg gatgcagcgc aaacatcgcc
    29461 accgggttgt aaccccagta gttactaagc cccatacgtt gcagacgtgg ttcactggca
    29521 aattgtgcca ccggcagcag ttccagcgcg gtaatgccca actgtttcaa atagttgatc
    29581 atcaccggat gccccagggc tttataagtg ccacggatct cgaccgggat ctccgggtgc
    29641 aggtacgtta atcctttgac atgggcttca taaatgatgg tgctgcccca cggcgtgcgc
    29701 ggcggggcat catcttccca gtcatagtga tcaatcacca ctacgcattt cggcgcaatg
    29761 gcggcgttgt cgcgatagtc aggttcatta tgaccggcgt gcagcagcgg gttatcttta
    29821 aactccccgt caatttgccg cgcgcaagga tcaatcaaca acttcgccgg gttaaagcga
    29881 tgcccctcgg caggttgcca ggggccatga actcgataac cataacgcaa acccgggcgc
    29941 gcatccggca gataaccgtg ccaaatgtcg ccactgtgcc ctggcaagtc atagcgatgt
    30001 tcctggccat tggcatcaaa gacacacagc tctacccgct cggcatgagc ggagaaaagt
    30061 gtgaagttga cgccctgacc gtcgtaatgc gcgccgaggg gagtgggttt gccaatggcg
    30121 agttgtgtca ttctgcctcc cgaaccagcc agatagtggc cagaggtggt agcgtcaggc
    30181 tcagtgaatg ttgacgaccg tggctggcaa tctcatcgct gtgtactgcg ccgccattgc
    30241 ctgcattact gccgtgatag tgcatggaat cggtattgag gatttcacgc catttacccg
    30301 gctggttaat accgaagcga taatcatgac gcggcaccgg tgtaaagtta ctggcaacga
    30361 tgatttcgtt accctcttta tcgcgacgca caaagatcag caccgagcgt tctttgtcat
    30421 ccaccaccag ccattcaaag ccgtacgggt caaaatccag ttcatgcatt gctttatggt
    30481 ggcggtaggt gtggttcaga tcgcgcacca gacgctggac accgtggtgc cagttatcgc
    30541 cgccttccaa cagatgccag tcgaggctgg cgtcatggtt ccactcgcgg ccctgggcaa
    30601 attcgttccc catgaacagt agtttcttgc ccgggaatgc ccacatccag ccgtagtagg
    30661 cgcgcaggtt agcgaatttc tgccatgcgt cgcccggcat ccggtcgaga atcgattttt
    30721 taccgtggac cacttcatca tgcgacaacg gcaggacgaa gttttcagtg tagttgtaga
    30781 gcatcccgaa ggtcagttta tcgtgatgat actgacgata aatcgggtcg agcttcatgt
    30841 agtccagggt gtcatgcatc cagccgaggt tccacttgta ccagaagccc agaccgccca
    30901 tatcctgtgg acgagaaacg ccagggaaat cggtagactc ctccgccatt gtcactgcac
    30961 cggaaacctg ctcaccaaga atacggttgg tattacgcaa gaattcaatc gcttcaagat
    31021 tctcgcgacc gccaaattcg ttcgggatcc actccccctc tttacggctg tagtcgcgat
    31081 aaatcattga cgccaccgca tcgacgcgca gcgcatcaat accaaaacgt tcaatccagt
    31141 aaagcgcgtt accgacaagg aagttactga cttcacggcg accatagttg tagatcagcg
    31201 tgttccagtc ctgatgatag ccttcgcgcg gatcgctgtg ttcatacaag ttcgtgccat
    31261 caaattcggc aagcgcaaag tcatcggtcg ggaagtggcc tggcacccag tcgagaatca
    31321 cgttcagacc agctgcgtgt gcggcatcaa tgaaataacg gaagtcgtcg cgagtaccaa
    31381 aacggcgggt cggtgcatac aggccggttg gctgataacc ccaactgcca tcgaagggat
    31441 gctcgttaat gggcagtagc tcgagatggg taaagcccat ccatttagca taaggcacca
    31501 gttgatcggc cagctcgcgg tagctcaacc agaaattgtt gtcggtgtgg cgacgccagg
    31561 agcccaggtg aacttcataa atagagattg gcgcatcaaa ctgattcgct tttttgcgct
    31621 cttcagtctg cacaaccttt tccggcagcc cgcaaataag agacgcggtt tccgggcgca
    31681 tttgcgcttc gaaggcataa gggtcggact tcagacgcaa gttgccattg gcatcaatca
    31741 tctcgtattt atagagctga ccgttatgcg ccccagggat aaacagttcc cagattccgc
    31801 tctctttacg caggcgcatc ggatgacggc gaccgtccca gtagttgaat tgcccaacca
    31861 ccgagacccg acgggcgttt ggagcccaga cagaaaaacg cgtacctgtg acgccatcca
    31921 tagtatctgc atgcgcgcct aaggtttcat acgggcgtag gtgagtacct tcagataata
    31981 gccaggcatc catttcctgg attagcgggc caaaacggta aggatcatca atcaggtttt
    32041 gctgaccatg ccagacaaca gccaactgat agcggaaaaa attcttacgt cgcggaatga
    32101 caccgctaaa gaatccccgt gagtcgagac actccagttt tgcgagtttg cgcccggttt
    32161 tcggttcaat cacccacaca tcggtagcgt cgggtaaaag ggcacggact tccagtcccg
    32221 cggtggtttt atgcatcccc agtacggaaa aaggatccgc aaaatggcct gcaattagcg
    32281 cgttaatcac gtctctatcg atacgatcgg acatgcttgt cttcctgttt tattgtgtca
    32341 ccccatccaa ctgaattttt gacttctggt tgtgacattt ttttgacctg aacggcgcag
    32401 cactctgtgc atcctctctg cgtcgtcctc acttcaggta aggctgtgaa tactcgtgta
    32461 ttccgccacc cttaaagaat agccaatgct ctatttaact cccggtaaat catgaaacat
    32521 ctgcgcttac tcctgtatta cgcactagca ggggcggcat cgcgccccag atttaatgaa
    32581 taaagattac gccagttgac gaagcatccg acgcagcggc tccgcggccc cccacagcag
    32641 ctggtcgccc acggtaaagg ctgacaggaa ctctggtccc atattcagct tacgcagacg
    32701 gcctaccggc gtggtcagcg tgccggtaac ggcagctggg gttagctcac gcatagtgat
    32761 ttcccgatcg tttggaacaa ctttcgccca cggattgtgc gcagccagca gttcttccac
    32821 ggtcggaata gacacatctt ttttcaattt aatagtgaat gcctggctgt ggcagcgcaa
    32881 tgccccgaca cgcacacata aaccatctac cggaattacg gaagatgtgt tgaggatctt
    32941 gttggtttcc gcctgccctt tccactcttc gcggctctga ccgttatcaa gctgtttgtc
    33001 gatccacgga atcaggctac ccgccagcgg cacgccaaag ttatccaccg gcagctcacc
    33061 gctacgggtt aaggttgtga ctttgcgttc gatatcgaga atagcagagg acgggttcgc
    33121 gagttcatca gccacatggc catacagatg gcccatctgg gttaataact cacgcatatg
    33181 tcgcgcacca ccgccggaag cggcctggta ggttgcaacg gacacccaat caacaagatc
    33241 attggcgaat aaaccaccca gcgacatcaa catcaggctt acggtacagt taccgccaac
    33301 aaaagtcctg atgccattat ttaatccgtc ggtaatgacg tcctgattga cggggtcaag
    33361 aatgatgatg gcgtcatctt tcatgcgcag agatgatgct gcgtcaatcc agtaaccttg
    33421 ccatccgctt tcacgaagct ttggatagat ttcgttggta taatcgccgc cctgacaggt
    33481 cacaatgata tcgagggcct ttagcgcctc cagatcaaag gcatcctgaa gtgtgccagt
    33541 ggttccgcca aaagacggcg cagcctgacc aagctgagaa gtagaaaaga agacagggcg
    33601 aatggcgtcg aagtcgcgct cttcaaccat gcgttgcatg agaacggagc cgaccatacc
    33661 gcgccagccg ataaaaccaa catttttcat aagcgttttt tcctgcaaag atgtgtgctg
    33721 tataaatgtg ccggtctcct cctggcacat ctttcaccat acaaaaagca gccaaagtcg
    33781 caagtgaaat taatcaatga tagcgaagcc atcagtaatg cgacttatcc tgctttgtta
    33841 gcacgcagaa agtccgcggc aattatcagg gaatttgagt tatgaatgaa atcatttctg
    33901 caacagtttt attgatcctg attatggatc cgctcggaaa cctacctatt ttcatgtccg
    33961 tactgaaaca tactgaaccg aaaagacggc gggcaatcat ggtgcgagag ttgcttattg
    34021 ctctccttgt gatgctggtg ttcctgtttg cgggcgagaa aattctggca tttcttagcc
    34081 tgcgggcaga aaccgtctcc atttctggcg gcatcattct gtttctgatc gccattaaga
    34141 tgattttccc cagcgcttca ggaaatagca gcgggcttcc ggcaggtgaa gagccattta
    34201 tcgtgccgtt ggcaattccg ttagtcgccg ggccgactat tctcgccacg ctgatgttgt
    34261 tgtctcacca gtacccgaat cagatgggac atctggtgat tgctctgctg ctggcctggg
    34321 gcggcacctt tgtcatcctg ctacagtctt cgctattttt acgtctgctg ggcgagaaag
    34381 gggtgaacgc acttgaacgc ctgatgggat tgattctggt gatgatggca actcagatgt
    34441 tcctcgacgg cattcgaatg tggatgaagg ggtaaggttc aaacaggtgt tgcacacaac
    34501 cagacgcggc acctgtttta ttacctgaac tgataatgac cttcgcccaa tcggataacc
    34561 cttccctctt cttctgcgcg gttcagccac acactaattt gttcgatggt taaaccagta
    34621 caagacgcca gcctttcaat acttatcgat tcctttgcaa ggatcgccag ttccgccaca
    34681 aagagttgat aaaaatcgac aggggtatgc gcgggcatta ctgcgtaatt tgcgtcatct
    34741 tcgtgcagcg aaaacagttc tggttgagcg ttacggagtg ctctgtcata catcagccca
    34801 ggaacgtctg gcagtctgat cgtcacattc tcagcctgtt cagcactcca tgacgcccca
    34861 ttttctacta atcgtgaatt ggctgattgc atatcctgat ttggtttaac ctgcatgggc
    34921 agccactgat gttttaatgc ctccatcgcc ccggttatcg ttccaccggt cattcccgca
    34981 cgtacaacca ttgcgctttc agcaaggcaa taaatatatt tattccgcgc catcgcattg
    35041 ccgacggtga aacgtacctc tgggtaaaac ggcgaaatca acaccaggtt gcctgctata
    35101 agcccttcac gccatttcac taacgtactc gtttttaaca agctatcagc taatacgcca
    35161 acggcagttc ccccgacctc cagagccgag gccattgcac attcatcaat acctcgcgca
    35221 ccaccagaga taacgcaaat cccctgttgg gccagtttcg cggccagttg ttgggtatag
    35281 cgcaaatcgt cagtcggagc atctcgcgag ccaacaatcg ccataccttc cgcttgcagt
    35341 aatgctttat tcccgcagcc aaataaaacg ggaggtgcat ccgttcgcaa tcggtttttc
    35401 aagcgaacgg gataatcggc atctccgcgg gttaagatcc ataaaccggc gcgttgccac
    35461 ttatctacag ccaacgccag actatgacca cgcgccagca agccaagaat tcgctcctga
    35521 gaaatacgcg gatcgctcca gcctgtaagt ttctcttgcg gttgcggcac cagaagctcg
    35581 gcgggactga tacgttggtg cttcagccat aatgcaaact tcccccattc actattacta
    35641 agaggtttat actcactcgc cgccgcgcga gaaaaatcgc tggttagcag gagagttgct
    35701 tgagcattgg ctgaaagatt catcaatttt ttaccgaggt agaagcaagg gcaagaggat
    35761 aaaccgtcgg gcaacctgcc tggcgtaaca gtgctgtcag aactgtcagc gtccatgcag
    35821 aatccacgat atcatcaacc agcaacgccg gacctggcat caaaggaggg gtaatcacaa
    35881 acgccccgtc gagattttga cactggtgaa aacggttttg ctgcattttc tgcggtggat
    35941 tgtccacgac tttttcaatg gcatcaataa aaggtaagcc aagtttcgcc gccagtcgcc
    36001 gggcaaaatc aggaaccagg tcgaggtgcc ttaatgaagg aacgcagcat acccaggtgg
    36061 gttcaggatg cggattccag cgttggcgaa ccatttccgc acatgcctcc accagctcat
    36121 cactgaagcg ccccgttttt ttctcctggg ctacctgctt tccccacccg gaatctcccc
    36181 aacgagaaag gattcgcccc gtagatcctt gtaaacccgc aggcaagttc cctttaaatc
    36241 cgtattgcgt aaaagcccca gatgccacct gtttattgag atttaatggc aggtcagcgt
    36301 gtttaataaa taaatttgcc gcatggagta agtcgctatc aatgtcgggc gacaacagcg
    36361 gatgctgaag acaactgcta catttaccac aacgttcggc acttggctca tcgagtgcgc
    36421 ggcgcagaaa ttgcattttg cactccttgg tcgtgatata gctttcaacg tctgccaact
    36481 cactttttct ctgattcatc aaatggttaa ttcgttcatg atcaggagaa aaactgacga
    36541 tagttctgcg ccataatttc tcggtataca ccacaggcga tggattttca gctaccaata
    36601 atttcagtgc tttttctatt tgcccataac gaagattcgt ccgttgttca atgcctcgta
    36661 gggtaagacc gtcattctcg ctaagtacgt tgagtatttc atgaatttgc gcctccgcag
    36721 gaaaggcgct ttcacggaag aattgatgaa tagcgcggtc ttcaccacca caaagcaata
    36781 tgccaactgc ggaatctata gcacgcccgg cacgccccac ctgttggtag taaccgacaa
    36841 tagaccccgg catctgataa tgaatgacga aacctaaatc aggtttatca aatcccatac
    36901 ctaatgccgt cgtcgcgacc agcactttga ttttatttgc cagcagtgcc tgctccagat
    36961 actccctggc agtattagaa tcttccgcac cttcacaagt cacgccgctg taataagcaa
    37021 atgcactgat accatttttc cttaaccact cggcgacaag ttcagcatcg cgagtcgtca
    37081 gggtataaac aattcccgat ttggaaaact gagggataac cgttgctaac catgccagac
    37141 gggatgactg ttctcctaag actaaggcat caagcgccaa actttcgcga gccagcgttc
    37201 cacgctgaat cacaatgtca cccaattgct gacggatatc ctcaacgaca cggttattcg
    37261 ctgtcgcggt tgtacccaga ataggggtat tcgcaggcag ttggcgcaaa atatctaata
    37321 tacgtcggta atccgggcgg aaatcgtggc cccaatcaga gatgcagtgc gcctcatcga
    37381 ccaccagtaa accgatgcgg tcagcgactg gatataaaac cgtttcaaga aaatcctgat
    37441 tcgccaaccg ttcaggtgaa atcagcagac aatctacgcc accttgcagc aacttatcgc
    37501 taatgcgctg ccattcttcc ctgtttgtag aattcaacgt ttcagccgta atacctaacc
    37561 gttcagcggc ggcaacctgg ttacgcatca gggcaagcaa aggagagatg ataattgtcg
    37621 ggccagcgcc gcgatcgcgg aagattttac tggcgatgaa gtacacggca cttttccccc
    37681 agccggtgcg ctgtacaacc agtagttttc gccgctgatt aaccaccgca tctatagctt
    37741 cccattgccc ttccctgaaa gtagcgctgg tgttacttaa catccgctgt aacagcaatt
    37801 cagctccgtg tttctccatc atcttctccc tcgtccgtta acacagtgat tagaacggat
    37861 aatgttccgg tctttttgcg agagagatca aaaagatgaa gacaggagaa aagagaaatg
    37921 aattactgcg agcaagctcg cagtaatttg tcgcagtaat taactcaaca gctggaacgc
    37981 aatcatccca acgatggcac cgacagtgcc gaggatggtt tccatcatgg tccaggtttt
    38041 aagcgtttcg gcttcggtcg cgccggtaaa tttaccgaac aaccagaaac ccgcgtcgtt
    38101 aacgtggctg acaacaatcg aaccaccggc gatacaaatc gacagtgccg ccatttgcgc
    38161 accggagtag ttcagttgtt caatgaccgg cattaccaat cccaccgccg ttaaacaggc
    38221 aacggtagca gaaccctgaa tgatgcgcac tgcagctgcc agcacgaagc aggtgatggc
    38281 aatcggcagg cccatgccgg ttaacgcttc gcccagtgcc ggaccgacac cagagtcaac
    38341 cagcacctgt ttgaacacgc cgcccgcacc aatcaccagc agaatgatcc ccgccggttg
    38401 cagcgcgtga ccgcaaatct ccatcacttt gtcttttggc ataccctgac gcattgccag
    38461 accgtaaatc gccaccaggc aagcaaccag aatcgcggta aacggatgac caataaactc
    38521 gaaccattcg taagcggtag aaccttctgg cacaaaacgc gcggcaatgg ttttcagccc
    38581 caccagcacc agtggcaaca ggatcagcga caggctgaat ccgaaggacg gcattttgcc
    38641 ttcgccgaga tgcggttcgc tgatgtcgtc aggaatatgc aactccacgt agcggctgat
    38701 gaaattaccc cacagcggcc cggcaataat cattcccgga attgccgcgc acaggccaat
    38761 caggatcatc cagccaaagt cggcgttcat ctgcgatgcc agcagcattg gcgctggccc
    38821 aggcaccagg aacgcagccg ctgccgccac gccggcgaat aatgggatta ccagcttcac
    38881 caggttcgtg ccggtgtggc gcgccattga gaaagcaacg ctaatcagca gaacaatcgc
    38941 cacttcaaag aacagcggta gcgcacaaac cagccccgca aggccgatgg cataatgcac
    39001 gcggctgtga ccgaaggatt tgagcatttt gacggcaatc tgatcgaccg cgccggtttc
    39061 atgtaagatc ttaccaaaca tggctcccag ggcgacaacc accgccagga agccgagggt
    39121 gcctcccatc cctttttcca tcgtcgctgc gattttatcg agcggcatac cggaaaaaag
    39181 gccagccccc atggacacca ccattaaagc caggaaagcg tgcatacgcg ccttcatgac
    39241 taaaaacagc agcagtaaaa cagaccctac tgctgttaaa acaagcgtta atgtagtcac
    39301 tacttatttg ccttttttaa taacctcaat ggtgcttgcc acaacacctt ccagcggttg
    39361 atcaatatcc accaccagta catcggtttc gtccgcaccc ggctcctgca gcgtttcaaa
    39421 ctgcgtcacc aacatttggg ttttaaagaa atggcctttg cgcgctttca ggcggctttc
    39481 aatcacatca aaatcgcctt tcaggtagat gaaagagaga ttaggattac cttcacgcag
    39541 caagtcgcga tagtgttttt tcagtgcaga acagacgatc agcgatactt tattggtgcg
    39601 ctgcatagca aacgcggcgt cgttcagcgc ctgcaaccac ggtttgcgat cgtcgtcatt
    39661 cagcggttcg ccagacgcca ttttttcgat attgcagcgc ggatggagga aatcgccatc
    39721 aagaaacgcg gcatgaagtt gatgcgccac ttcactggcg accgcagatt tgccgctgcc
    39781 cgatacgccc atcaagacgt aaatgtggtg atcatggtta gtcgtgctca aagtggtgcc
    39841 cccacaatac aagaattaaa aatgttacgg gtaactgtta tcggtaacat tgtccagccg
    39901 gacaatatca gaagcaatat ccatccgtgc cttaagtgta taagtgtgag ctacttcaaa
    39961 tttgtaggct taaatagatc cgcccggtga caaggtgaaa cctaaatcta acattttcgg
    40021 tgtcacagat tcgccacgaa tacgcgccag caggcgctca gcgccaatgc tgcccatccg
    40081 ctcacgcggc gtcagcacac tcgccagccg tggctccatc acctgaccaa tgtcatgacc
    40141 gtggaaaccg gcaatcgcca tatcgtcagg aactttcaac cccagacgct gacattcaaa
    40201 cgccgcgccg accgccaggt catcattagt acagaacacg ccatccagct gcggatattc
    40261 ccgccgcgcc tggcgaatca gttcaatacc ggaagagtaa gaagaagatt gctcaaccat
    40321 cacgctatac ggcaccaggc ctgcatccag catcgcctgt tcgtatccct tctgtttgat
    40381 gatagtacgt tcgtcgagac gtgcgccgag ataggcaatg tggcgatgcc cgcgagcaat
    40441 aatggcggtg gtcatctggc gtgccgcttc aaagttatca aaaccgacgg cgatatcgag
    40501 acacggcgac ttactgtcca tcaactccac caccggaatc cccgccactt caatcatctt
    40561 taaggtgcgt ggcgtgtggg tacgttcagt gaggatcagg ccgtcgatat tccatgagag
    40621 cattgattcg aggcgttctt gctccatttc cggtttataa ccgtagtgcg ccagcatggt
    40681 ttgataaccg tgcgcgtcgg tgacactttc gattccgcgt aatacttccg cgaaaacctg
    40741 gttggtgaga gaaggtaaca ggacgccaat cgcccggctg gtggcgttag agagtatatc
    40801 gggcgcgcga ttgggaatat aacccagttc atcaagagcg gcggcaatct tgccgcgtag
    40861 agcgacggaa acctgctccg ggttgcgtaa aaaacggctg accgtcattt tggtcacgcc
    40921 tacacggtca gccacatcct gaagtacggg tcttttcttt ttcatcgtcc tgaaggtaca
    40981 aaagagatag attctcttag tttaacacgg acgcatcaca accttcctga ttcctcagga
    41041 aggtttgtga gataaattta gactggcggt aaatcgaaca gcaggatttc actgtcgcta
    41101 tcagcatgga tggaaatcgc ctgctcatcc cagattgcca gaccatcgct ggtcgaagct
    41161 tttacgccgt taatggtgac attgcctttc accacctgga tccagatgcg gcgttcagcg
    41221 gcaatctgat gcaccgactg ctcatctttc accagcgccc aacgatacag ttccatatcc
    41281 tgatgcactt tcaatgaacc atctcgcgca tccggcgaaa gcaccagttg tttgccctgc
    41341 acggcatcga agcgacgctg ttcataacgc ggcgtaatac cgttttcttc aggcatgatc
    41401 cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga gtgacgaata
    41461 cccgtacccg cactcataat ctggaactcg cccgccggaa cctgctcttt attgcccatg
    41521 ctgtcctggt gctcaacagt accttccagc acgtaggtca aaatttccat atctttatgc
    41581 gggtgagtgc cgaagccctg ccctgcttca ataacgtcgt cgttaatcac gcgcagcgca
    41641 gagaagccca taaagttcgg atcgtaatag ttggcaaaag agaaagtatg ccaggagtcc
    41701 agccagccat gatttgcatg accgcgttca tttgctttgc gtaagtagat catttgtatt
    41761 cgccccctga atgatttcga tgggcttagt gtggacccga tccgcctggg atgatagagg
    41821 gtgaaaattg acccctctgt tcaaaaaaat tgaactattc gaggggccat caaacttacc
    41881 tcgcgagact cactgcggag ggagaagctt gctcaaaacc gcgttcaagg atttccagat
    41941 tcgtaagaac ttcagattcc ttgacgtaat ttggcgcacc gttggtaatg gtttgataca
    42001 acgcatcata aacgcgccca taatcgccca tctctggctt catctcttct cttaccgtca
    42061 cgccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg aatcctggtt
    42121 cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg tatttaataa
    42181 acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc agatggctgg
    42241 ttttgacgat ggcttttaga tcgccataaa acagctgcgc ttcaaaggtg tcgtccggat
    42301 tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga ccgaacagag
    42361 aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc tgcggcagcc
    42421 ccggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact tcgacaatct
    42481 ctcccagctt gccgctttca atcgcttttt tcgctgtcag gaagcaggaa tcaaagcgac
    42541 gattctgata cggcgtaacg atcagccctt tgcttttcgc cagtgcaaac agctctttcg
    42601 cctgcgcaat tgtcggagtg aacggttttt cgaccagcac atttttcccg gcttccagcg
    42661 cgcgcttcgc gtactcgaag tggctgtccg cgtgggtgca gacgacaacc agcttaacat
    42721 cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgggaa taaatggggg
    42781 cctgttcttc cggcttcgca tggcggcgaa aaatatgcgc gacatgccag ctatccttgc
    42841 ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca ataaaggcgc
    42901 agttaatgac catgatttcg tcctttttaa ggtggttatt tacaccttag cgcaaagcgg
    42961 acgtggttcc tactggagtg cgcataacga acacaagcac ttcccgagga taaattgaga
    43021 acgaaagatc aaaaaaaaag ccagcacccg gctggctaag taatactgga agcaatgtga
    43081 gcaatgtcgt gctttcaggt tctccgcgag ggtcttcctg atcgcgagac aataataatc
    43141 attctcattc gcacttgtcc aacacttttt gcaaaaaaat gcatttgact cgcatttgaa
    43201 agtcaatgat gttgaaaggg acatttaccc caaagaggac aaaggaatga gtgagatagt
    43261 aatacgccac gcagaaacac gcgattacga ggccatcagg cagattcacg cccagccgga
    43321 ggtgtattac aacacgctac aggtgcctca tccttcagaa caaatgtggc tggaacgact
    43381 caccgctcgc cctggcatca agcagttagt cgcctgtatt gatggaatcg tcgtaggcca
    43441 tctcaccatt gacgtgcaac aacgcccacg ccgcagtcat gttgccgatt ttggtatctg
    43501 tgtcgactcc cgttggaaga accgcggcgt cgccagcgcc ctgatgcgag agatgattga
    43561 aatgtgcgac aactggttgc gggtagatcg tatcgagcta acggtgttcg tcgataacgc
    43621 gccagcaatt aaggtctata aaaaatttgg ctttgagatt gaagggactg gcaagaagta
    43681 cgcattacga aacggtgaat atgtcgatgc gtattatatg gcgcgtgtga aatagagaat
    43741 ttgcaatcta cgtttgtcat ttaagccctc acctaccggg agagggctta agatattaat
    43801 accccgccgt taaatcatcc accgagcgcg ggtcggatgc accgtacagc tcaccgtccg
    43861 gcccaaccat aatgctttgt gtactgccca tcgcctcttt cagcgccact ttctgacctt
    43921 ttgcttccag cagcttgatc gtatccgggc taaacccttt ttcgacacgc agctcgtccg
    43981 gcaaccactg atggtggaaa cgcggcgcat tggtcgcttc ggcgacgttc attccataat
    44041 cgatgctatt caccaccatt tgcagcacag tggtgataat ccggctaccg cctgggcttc
    44101 cggtgaccag ccaggtttta ccatccttga ccacaatggt tggtgacatc gacgacagcg
    44161 ggcgtttatt cggcccgacg gcgttggcat cgccgcccac cagcccgtaa acgttcggta
    44221 cgcttggttt ggcggagaaa tcatccatct ggttattgag caaaataccg ctctcgcccg
    44281 cgacaatgcc cgtaccgaag gtggtgttca gtgtataggt caccgccacg gcgttaccgt
    44341 ctttatccac tactgagtaa tgggtagttt gattactctc ataaggcgca agcttgccag
    44401 gacgaatttc gctggatggc ttcgctttat tgatatcgat ttgatcggca atagatttgg
    44461 cataggcttt attggtcagc gcctgccacg gtactttgac aaaatccggg tcgccaagat
    44521 attccgagcg gtcggcgtag gcgtatttct cagcttctgc catgatttgc atcgcgtcgg
    44581 cgctgccaaa gccgtacttc tgcatatcga agttttccag aatattgagg atttgtacga
    44641 tatgaatacc gccggaagat ggcggtggca tggagtaaac ctgatacccg cgataatcgc
    44701 cgcttatcgg tgtgcgttcg accgctttat atgccgctaa atcttctttg ctgatcaagc
    44761 caccgttttt ctgcatctcc tgggcaatct gttccgctat cgtgcctttg tagaattcat
    44821 ccgggccgtt ttcggcaatc atctccaggc tctttgccag gttcgcctgc accagcttgt
    44881 cgcccttttt cagcggttcg ccctctttcc agaagatagc tttgctgttt tcgtggttcg
    44941 gcagcacttc actgccgtag gttttgagat cgtcagccag cgcgtcgtta acgataaaac
    45001 catcgcgtgc cagtttaaac gccggttgca ccactttatt caacggcatg gtgccgtatt
    45061 tatccagcgc caacgagaaa cccgctaccg taccgggtgt gccggaagcc agatgcgaag
    45121 tgagtgattt tttgctgtcc gtgttgccct gatcatcgag gaacatatcg cgggtcgctt
    45181 tggcgggtgc catttcgcgg aaatcgatag ccgtggtatt gccatttttc gagcggatca
    45241 gcataaaacc accgccgccc agattccctg cctgaggatg cgttaccgcc agcgcgtagc
    45301 ccaccgccac ggcagcatca acggcattcc cgccctcctt gaggatatcc actcccacct
    45361 gagtggcagt aacatctaca gacgccacca tcccttgttt cgcgcgtacc ggatggaaga
    45421 catcttcctc cacaccataa gaaacaggag gcgcggctac cgtactaaaa caacttcctg
    45481 tgagcagagc agcaatggcc acccggcgta aaaacgtcgg ttttatcatc gttattctcc
    45541 agagattaag gggcaacccc agctaagcct ggtatataac tctgaattaa tcatcgtttt
    45601 gccgggaagc gagtaaactt aaagaatatc ttcagaggag gatacgcgat gaaacgactt
    45661 ctgcttttga cggcactcct gccgtttgtc ggctttgcac agcccattaa tactctgaac
    45721 aaccctaacc agccgggata tcagatcccc agccagcagc ggatgcaaac tcaaatgcag
    45781 acgcagcaaa ttcagcaaaa agggatgctg aaccagcaac tgaaaacgca aacgcagctg
    45841 caacaacagc atttagaaaa ccagataaac aataattctc agcgggtgtt gcagtcacaa
    45901 ccgggggagc gaaatcccgc ccggcagcaa atgctgccca acaccaacgg cgggatgtta
    45961 aacagcaacc gtaatccgga tagttcgttg aatcagcagc atatgctgcc ggagaggaga
    46021 aacggcgaca tgctgaatca gcccagcacg ccgcagcctg acattccgtt gaaaactatt
    46081 gggccgtaaa gttcggacca atcacgtcaa tcgcatcggt acagatgcaa tccacgcccc
    46141 agcgcagcaa ctctgccgcg cgctggggtt tattgacggt ataaaccaga attcgcagtc
    46201 ctgcatcttt caactgcatc actcgcgctt tatcgagcaa cttataattg agatgaatag
    46261 agacgcagcc cagccgcgcg gtcaattctc gccagtcgtc gcgccattca tcaagcaata
    46321 aaccacgcgg cagttccggt gctgcctgtt gagcggcttc taaagcatca atctcaaacg
    46381 atgacagcag cggcggcgtc ataccggtcc acagttcgcg tgccgccaac gccaccattt
    46441 tgcccgttaa tggtccagtg tcagtggtgg gtttgatttc gatattcgcc atcatcccgt
    46501 gtttgcgaca gcgttccgcc acctgcgaaa gcaacggcag tggctcacct ttaaacgctt
    46561 tgctgtacca actgcctgca tccacgcgca gtaaatcctg ccagttcagt tcacccgcga
    46621 cgccccagcc gttgctggtg cgttcaagat tgtcgtcatg gagcaggaag atctcgccat
    46681 ctttcgataa cttcgcgtca aattcgatca tcttatgacc gtattttgcc ccgacgtcga
    46741 ttgctgccag ggtgttctcc ggggccagtt taccgccgcc acgatgggcg acgatgcggg
    46801 gataaggcca gttactcata ctcgttgtcc tgtttcacca tcaaaaagat gtagctgatt
    46861 ttccggcaga tgcagccaca gcgtgctgcc tgccgtcggg cgctcctgat gtgccagtcg
    46921 caccaccagc ttctgttcgc cccaatgtcc gtgcgccagg ttatctgcgc cgaggatctc
    46981 cagcgtgtcc atcaccagcg gcacaccacc ttctgcctgc gagcttagcg ctatatgttc
    47041 cgggcgaatg ccgagggtca ttttacgccc gacatactga cggtagccac cgtttagcgg
    47101 cagcgcaata ccgccatcca gttcgaaatg cgtaccttcg ttattcacac ggccagccag
    47161 taggttcatc gccgggctgc cgataaaact cgccacaaac aggctggcgg gcttttcgta
    47221 cacttcaact ggcgtgccaa tctgttcggc aacgccgccg ttcatcacca ttactcgctg
    47281 ggcgagcgtc atcgcctcaa cctgatcgtg ggtaacgtag agtgaagtcg ttttcaggcg
    47341 acggtgcagc tgttgcagtt caagacgcat ctgcacacgc aacttggcat cgaggttaga
    47401 gagcggctca tcaaatagga acactgccgg ctcacgcaca atcgcacgcc ccatcgccac
    47461 acgctgacgc tgaccgccag atagctcacg cgggcggcgc ttaagcaggc cgtccagctc
    47521 cagaatgcgc gccgcttctt taacacgttc ggcaatttgt tgcttgccca tgccgcgaat
    47581 tttcagcccc cacgccatgt tctcctctac gctcatatgc ggatagagcg cgtagttctg
    47641 gaacaccatc gcaatcccgc gatctttcgg ctccatttcg gtgacacgct tgcggtcgat
    47701 ccagatatcg cccgtcgtta cccgctccag tccggccacc attcgcaata acgtcgattt
    47761 cccgcagcca gacggcccga ccatcacgat aaattcgcca tccgccacat caagggtcag
    47821 cggtttaatg acctgggttt tgccatccca gcttttggtt actgcctgta atttcagtcc
    47881 tgccatctta tttctcacta tcgaccaggc cgcgcacgaa ggcacgctgc atcactaaaa
    47941 caatcactac tggtgggatc agggttaaca acatcgccgc catcaccgag ttccattccg
    48001 ttgtgccttc gcctgtggcg atcatccctt taatccctgc cacggtggtg ccgagatcaa
    48061 cgtcggtaat aatcaacaac ggccacaaat actggttcca gccgtaaata aaggtgatca
    48121 caaacagcgc cgccagattg gttttggaca gcggaaaaac gatgtcgcaa aagaaacgca
    48181 ttggcgacgc gccgtcgatc cgcgcggctt ccaccagttc atccggtagg gtcataaaga
    48241 actggcgaaa cagaaacgtg gcggtcgccg aggccatcag cggcagcgtt aaacctgcgt
    48301 agctgtcgag cattttcagg ttggcgatga cttccaccgt cgggaagata cgcacttcaa
    48361 ccggcagcat cagggtgata aaaatcatcc agaagaagag gttacgtagc ggaaaacgaa
    48421 accagacaat ggcaaatgcc gagagcatcg agacggtaat tttgccgagc gtaatgctga
    48481 acgccatcac aaagctgtta agcaacatcc gccagaacgg cgcgctattc gtgcctaccc
    48541 cgtttaccca gatgttgtgg atgttttcca gcagatgtgt gccgggaata agcgtcatcg
    48601 gcgcggcgta gacttcttgt ttatccagcg tcgccgcgac aaacgccacg tacagcggga
    48661 agaggatcac cgcgatcccg aggatcagca tggtatggct gaatattgtc agccacggac
    48721 ggttctcaat cattggtaac gtaccttgct ttcaacatag cggaactgca ccaccgtcag
    48781 cacgatgacg aggaacatca acaccaccga ctgcgcggca gacgaagcca aatccagtcc
    48841 ggtaaagcct tcacggtaga tcttataaat cagcgtcgtg gtggcctgta ctggcccgcc
    48901 ggacgtggcg gcgtcgatca ccgggaaggt gtcgaagaag gcatacacca ggttcaccac
    48961 cagcaggaag aaactcaccg gagcgataag cggcagcgca atcttaaaga agcggcgaat
    49021 cgggccagcc ccatcaatgg ctgcggcttc gatcaacgaa cgggggatgg attgcagcgc
    49081 ggcatagaag aacagaaagt tgtagctgat ttgcttccac actgaggcaa acaccaccag
    49141 gaacatcgcc tgaccgctgt tttgcgcgtg gttccagtcg tagccgaact cagcgagaaa
    49201 atgggtgatc agcccgcgac cggggttaaa cagaaagatc cacaatacgg cggcaacggc
    49261 gggagccacg gcatacggta gcaacatcaa ggtttgatag aaacgactgc cgcgcacgat
    49321 gtactccacc agcgctgcga agaacagcga taccagcaaa ccgctgacgg tgacaaaggt
    49381 gctgaatttt atcgtcgtcc agaaggcgtc gagatagtag ctgtcgtgaa acagcgtgac
    49441 gaaattatcc aggccgacaa actggctgga gaaaccaaac ggatcgacgc tttgtagcga
    49501 gtaccacaac gcttcgcccg caggccagat aaaaaagata acggtgatga tgagctgcgg
    49561 cgcgaccagc aaataaggta gccagcgcga gcggaacacc ggacgggatg atggcattac
    49621 tgttaattcc tgaactgtgc cggatgcgct tcgcttatcc ggcctacacg cgatgccagg
    49681 tcagataagc gaaacgcatc cgacattaca ctgattaaga cttcgtcgat ttctcaaagc
    49741 ggcgcagtaa ctggttccca cgctcaacgg cagtatccag cgcctgctgt ggtgtcttct
    49801 taccggtcca cacgctctcc agctcttcat ccacaatcac gcggatctgc ggcatgttgc
    49861 ccagacgcaa acctttggtg aacggcaacg gtggcttgtt cagcatctga cgcgtggcaa
    49921 tatccgctcc tgggtttttc tcgtaaaagc cctgctcacg ggtcaggtca tacgccgctt
    49981 tagtgattgg tagataaccg gttttctgat gccactcggc agcgttttct ggttttgcga
    50041 ggaagtcgag gaacttcgcc acgccggtgt aagtttcttt atctttaccc tgcatcaccc
    50101 acaggctggc cccgccgata atggcgtttt gcggcgcgtc tttcgcatcg gcgtcgtaag
    50161 gcatcatgcc tacgccatag ttaaatttgg cgtactcgcg aatgttggca agagaaccgg
    50221 aagaggcggt cgtcatcgcg caatcaccgt tatagaactt ctcggtggat tcatccttac
    50281 gcccaacgta gctgaaatcg cccttcttgt tcatctcttc gagcatggcg atgtgtttca
    50341 cctgctccgg cttgttgaac tcaagcaccg cgtctgtgcc gtcaaagccg ttgtttttgc
    50401 tggcaaacgg cagaccgttc caggcgctaa agttttccag ttggatccag ccctgccagc
    50461 cgctggcgta gccgcacttc atgccggagg cttttagttt tgcggcatag tccgccagat
    50521 cctgccaggt ttttggcggc tgttccgggt ctaatcctgc tttcttgaag gcgtctttgt
    50581 tgtaatagag aacgggggtc gaactgttga acggctggga aagtaagtgc ccagttttgc
    50641 tgtcggagta gtaaccggaa accgtcggca caaactgcga ctcatcgaat tgaatccttg
    50701 cctctttaaa tacgtcatac accggtttaa tggctttcga cgccatcatg gtggcggtgc
    50761 caacttcata aacctgcaaa atagccggag cgttgccggt acgaaatgcg gcaatccccg
    50821 cgcttaaatt ctgttcgtaa ttgcctttat aggtcggtac aattttgtaa tccgggtttt
    50881 cggcgttaaa acgttgggcc agagaatcca cctctttacc cagttcccct tccatagaat
    50941 gccagaacgg aatggtcgtc actgcctgcg catttgccat taaagccagt ccgagcgcca
    51001 gtgctgaagc tgtataacgt aacggtttca ttgattatct ctcttgttgt accgaatgcg
    51061 cgaattcacg cgttttatgc tcgcggagta acatgacatg ctcgaattac agaaaaataa
    51121 ctattttgtt acatttttaa gatagtaagg tgtcagaaag atgacaaggc ggtgacggcg
    51181 cgggcgaggg aaaatgggag atggggcacg gataagcgga aaaatagaag gtctgaatca
    51241 aactctacag attactcatc gtttcatgcc ggatgcggcg taaacgcctt atccggccta
    51301 caagatcgtg caaattcaat atattgcaac tcacccagta ggcctgataa gcgcagcgca
    51361 tcaggcaatt ttacatttgt cacctgtctc aaaggagtct tttgactcct tatcaatcaa
    51421 cgtgttatta cccgcctaaa tatgcacttc tcactgcttc attcgccagc agcgcgtcgc
    51481 cggtatcgga gagcactaca tgaccgtttt ccagcacgta gccgcgatca gccagcttta
    51541 gcgcctggtt ggcgttctgc tcgacgagga agatggtcat cccctgctcg cgcagttgtt
    51601 cgatggtgtc gaaaatttgc tggatgataa tcggtgccag cccgagcgat ggctcatcaa
    51661 gcagtagcaa acgcgggtta ctcatcaaag cgcgaccaat cgccagcatc tgctgttcac
    51721 cgccagacat agtgcccgct cgctgaacac ggcgctcatg cagacgcgga aacagttcat
    51781 agacccactt tatgcgctcc tggaactggt cgcgttcggc aaaaaagccg cccatcgcca
    51841 ggttctcttc caccgtcatc cgcgagaaga cgcgacgccc ttccgggaca atcgctaccg
    51901 cttcgcgcat gattttcgcc gtctgccagt cggtaatgtc tttatcatca aacacaattc
    51961 gcccgctggt ggcgcgcgga tcgccgcata acgtgccgag caaggtggtt ttccccgccc
    52021 cgttcgcgcc aatgagggtg acaatctcgc cctgattgat atgcaggctc acctcatgca
    52081 gcgcctggat tttgccgtag tgggcgctga ctttgtcaaa ggacaacatg actttttcca
    52141 tcttatgcct cacctaaata ggcacggatc acatctgggt tattacggat ctgctccggt
    52201 gtaccgtttg ccagcggcat cccctgattg accacgtaaa ttctgtctga aatccccatc
    52261 accagcttca tatcgtgttc aatcaacaag atagtggtgt tgtgatggtt acgcagttcg
    52321 gcaatcagct catccagctc tttcgtctct ttcgggttaa gacctgctgc cggttcgtcg
    52381 agcattaaaa tctccggctg cgtcaccata cagcgggcaa tctccagacg gcgctggtca
    52441 ccataggcca ggttactcgc ctgacggttg gcatgttcca gcaaaccaat gcgctcaagc
    52501 caggtggcgg cgcggtcgag tgcttcgctc tgggcgcgac ggaaagaggg ggttttcaac
    52561 aggccggaga acagcccagt tttcagttgc tgatgctgtg ccaccagcag gttttcaatc
    52621 accgtcattt cacggaacag acgtacatgc tggaaggtgc gcaccacgcc catgcgggca
    52681 atttgctgcc ccggtaaacc ttccaggtgc tgatcgcgca gtaaaatggt gccgccggtg
    52741 ggtttataga atccggtcag gcagttgaag accgtggttt ttccggcacc gttagggccg
    52801 attaacgaga cgatctcctg cgggtacagt tcaagattga cgttgttcac cgccagcagg
    52861 ccgccgaagc gcatcatcag gccgttaaca gataataatg gctgactcat gcctgctctc
    52921 cttttgctgc gccgtttttc agcttgagtt gcgggcgcgt catgggcagc aagccctgcg
    52981 gacgccagat catcatcagc accatcaaac caccgagcat taacatgctg tattcgttga
    53041 aatcacgcat caactcgcgc gataccacca gcaaaacagc cgcaagaatc actgcaaatt
    53101 gcgagcccat accgccgagc accactatcg ccagcacaaa cgccgattcg gcaaaagtga
    53161 aggattcagg gctgacaaag ccctggcgcg cagcaaacaa cgttccggca aaaccggcaa
    53221 acgcagcgct gatggtaaag gcagtcagct tgatacgacg cgggcttaag cccagcgaac
    53281 gacaagcgat ttcatcttca cgcaacgctt cccacgcacg ccccagcggc atccgcagca
    53341 ggcggttaat gacaaacagg cttaacacca ctagcagcaa cgccaccagg tagaggaaga
    53401 tgacgcgatc ggagggatcg tatttcaggc caaagaaatt gctgaacgtg tcccagccgc
    53461 cttcgcgggc ggtacggctg aactcgagtc cgaagagcgt cggtttcgga atctgactga
    53521 taccgttcgg tccgccggta atttcggtgt tattgagcag caatatgcgc acaatttcgc
    53581 cgaacccgag ggtaacgatc gccagatagt caccgcgcag acgcagcacc ggaaaaccga
    53641 gcaggaagcc cgccgccgcc gccattaacc cggcaatcgg cagacaggtc cagaagccca
    53701 ggccgtaata gtgattgagc agcgcaaaag tgtaagcgcc gatggcgtaa aagccgccgt
    53761 atcccagcac cagcagacca gaaagaccaa caaccacgtt cagcccgaga ccgagaataa
    53821 tgtagatcat ggtcagagtg gcgatatcca ccgtcccacg cgacaccata aacggccacg
    53881 ccactgcaag caccagcagc gccacgagga acagtttctg cttcaccgtg gagccatcaa
    53941 tcgcgggcag aataaacttc ggcccggaaa cgctttttag ccctttctgg aaagcaggcc
    54001 gcacaagctg gaagaaaaag accaccgccg tgccgataaa cacccactgc caacggatct
    54061 ctgaagccgt gtcgaccacc agtttggtgc catccagctc cagttgcacg cccataaaga
    54121 cgcccgccag cacaaagaac atagcggcag agagcagcgc cattgcaata tgcatcggtt
    54181 tcatactttc tctacctccg ggcgacccag aataccggtc ggcatcacca gcagcaccag
    54241 gatcagcagg gcgaatgaga ccacatcttt atattccgta ctcagatagg cagaagagag
    54301 cgcctccgca atccccagga tcagcccgcc aatcatcgcc cccggaatgc tgccaatccc
    54361 accgagcacc gcagcggtaa aggctttcat cccggccata aagccgatgt aggggttaat
    54421 tacgccgtag aactgaccga gcagcacgcc cgccactgcc gccatcgccg cgccaatcac
    54481 aaaggtcagc gcaatcaccc ggtcggtgtt gatgccaagc aggctcgcca ttttcagatc
    54541 ttccgcgcag gcacggcagg cgcgccccat acgggaatag cgaatgaaaa tcgtcagcgc
    54601 cagcatggcg aggaaagtaa caatccatat caccgcctgc atggtggtaa tagacgcaga
    54661 gaagttttcg ctatggccca ccacccactg accgttaaac aggctgggca gcgccacatc
    54721 gcgcgaacct tcggtcaggc tgacgtagtt ttgcaggaag atagacatgc cgattgccga
    54781 gatgagtgca atcaggcgct tagagttgcg caccgggcga taggctaccc gttcgatact
    54841 ccagccgtag gcgctggcaa tgacgattgc gccgacgaat cccgccgcca ccagcagcca
    54901 gccggtatca atgcccagca tcatcagtgc ggcgatgatc ataaaggaga cgtagctgcc
    54961 aatcatgtaa acctcgccgt gggcgaagtt gatcatgccg ataatgccgt aaaccatggt
    55021 gtagccgatg gctatcagcg cgtaggtact gcccagcgtg acgccgttaa acatctgctg
    55081 caagaaatac aaaaactgct cagacataag gtaacctttc taaacccgcc cgtttttcac
    55141 ggacggtggg atgatcactt ggctgccgtg gatgaaccgt cggcgtgcca ctggaagaca
    55201 ccaaaatcga atcccttaag atcgcctttt tcatcccagt tcagcggccc aattacggtg
    55261 tttgcaccgt tagcttttaa atctttcacc agcgccagcg gctcatcgct gccggtacgt
    55321 tcaagggcag ttgccagaga ttgcaccgcc gcgtaggtga tccagacata gggcccggac
    55381 ggatctttct tgtcggcttt cagcgcatca acgatgccct ggtttgtcgg atcctggtca
    55441 tagcgttttg gcatggtgac caacatgcct tcagcggcat caccggcaat gttcgacagt
    55501 gacgcattac cgacaccttc cggccccata aactgggttt tcaggccaac ggaacgggcc
    55561 tggcgcagca tctgccccat ttccgggtag taaccgccgt agtaaacgaa gtcgatgttt
    55621 tcttttttca ggcgggcgat cagcgcggag aaatctttct ctccagcggt aatgccgtcg
    55681 aagaagacga cgttggcgtt agccgctttc agcccgtcct gcaccgaacg tgccagccct
    55741 tcgccatact gttgtttgtc gtgaatgatg gcgatgcgct ggggcttcac cgtctcaaga
    55801 atgtattttg ccgccgttgg cccctgggaa gagtccagcc cggcagtacg catgatgtat
    55861 tgataaccgc gttgggtcag ctccggattg gtcgctcccg gcgagatcat cagaatacct
    55921 tcgtcttcat agatatctga tgcaggttgg gtagaggagg aacacaagtg cccaataacg
    55981 tatttaatgc cgtcgttaac gattttgttg gcgaccgcaa cggcttgttt cgggtcgcag
    56041 gcgtcgtcat attccacgcc aaccagttta tcgcccttaa ttcccccttt ggcattaatg
    56101 tctttaattg cctgacgcgc gccgttaaat tccatatcgc cccactgggc aatcgggccg
    56161 gacatcgcgc cgacaacggc gactttaata tcgtcagcca tagcggtgtg tgaaattgcc
    56221 agtgcaatca tccctgcgat gatagttttc gcattccgtt tcatagtcaa aaatccccat
    56281 tcgtgatgtt gtgttgcttt gttcttatgt gttaacaaat cagactgttc ttttttatac
    56341 tgcactgttt ttacctg
//
LOCUS       DACADF010000009        55118 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.009, whole genome shotgun sequence.
ACCESSION   DACADF010000009 DACADF010000000
VERSION     DACADF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.009"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            320..2569
                     /gene="fhuA"
                     /locus_tag="ISB30_000548"
     CDS             320..2569
                     /gene="fhuA"
                     /locus_tag="ISB30_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAP1988227.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKMQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGAEEQRYAIAPAFTWRPNDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYSSNPSSSPCANVPQSQWGHTLTRQYVIDNEKLENFS
                     VDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFDFGA
                     HPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSERDD
                     KEFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYVPED
                     RPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNLVGSYT
                     YTDAEYTTDTNYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPA
                     NSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQ
                     VVATATFRF"
     gene            2621..3418
                     /gene="fhuC"
                     /locus_tag="ISB30_000549"
     CDS             2621..3418
                     /gene="fhuC"
                     /locus_tag="ISB30_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAP1988228.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILIDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGVADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            3418..4308
                     /gene="fhuD"
                     /locus_tag="ISB30_000550"
     CDS             3418..4308
                     /gene="fhuD"
                     /locus_tag="ISB30_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAP1988229.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            4305..6287
                     /gene="fhuB"
                     /locus_tag="ISB30_000551"
     CDS             4305..6287
                     /gene="fhuB"
                     /locus_tag="ISB30_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAP1988230.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGMVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPTGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(6322..7602)
                     /gene="hemL"
                     /locus_tag="ISB30_000552"
     CDS             complement(6322..7602)
                     /gene="hemL"
                     /locus_tag="ISB30_000552"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAP1988231.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMSCDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            7827..9248
                     /gene="clcA"
                     /locus_tag="ISB30_000553"
     CDS             7827..9248
                     /gene="clcA"
                     /locus_tag="ISB30_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAP1988232.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            9272..9337
                     /locus_tag="ISB30_000554"
     CDS             9272..9337
                     /locus_tag="ISB30_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988233.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            9330..9674
                     /gene="erpA"
                     /locus_tag="ISB30_000555"
     CDS             9330..9674
                     /gene="erpA"
                     /locus_tag="ISB30_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAP1988234.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(9721..10344)
                     /locus_tag="ISB30_000556"
     CDS             complement(9721..10344)
                     /locus_tag="ISB30_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAP1988235.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(10382..11182)
                     /gene="btuF"
                     /locus_tag="ISB30_000557"
     CDS             complement(10382..11182)
                     /gene="btuF"
                     /locus_tag="ISB30_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAP1988236.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(11175..11873)
                     /gene="mtnN"
                     /locus_tag="ISB30_000558"
     CDS             complement(11175..11873)
                     /gene="mtnN"
                     /locus_tag="ISB30_000558"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP1988237.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            11957..13474
                     /gene="dgt"
                     /locus_tag="ISB30_000559"
     CDS             11957..13474
                     /gene="dgt"
                     /locus_tag="ISB30_000559"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAP1988238.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLETYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            13604..15028
                     /gene="degP"
                     /locus_tag="ISB30_000560"
     CDS             13604..15028
                     /gene="degP"
                     /locus_tag="ISB30_000560"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAP1988239.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGPGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            15183..16340
                     /locus_tag="ISB30_000561"
     CDS             15183..16340
                     /locus_tag="ISB30_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HAP1988240.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(16394..16780)
                     /locus_tag="ISB30_000562"
     CDS             complement(16394..16780)
                     /locus_tag="ISB30_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAP1988241.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(17090..17914)
                     /gene="dapD"
                     /locus_tag="ISB30_000563"
     CDS             complement(17090..17914)
                     /gene="dapD"
                     /locus_tag="ISB30_000563"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAP1988242.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(17945..20617)
                     /gene="glnD"
                     /locus_tag="ISB30_000564"
     CDS             complement(17945..20617)
                     /gene="glnD"
                     /locus_tag="ISB30_000564"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAP1988243.1"
                     /translation="MNTLPEQYANTALPTLHGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(20679..21473)
                     /gene="map"
                     /locus_tag="ISB30_000565"
     CDS             complement(20679..21473)
                     /gene="map"
                     /locus_tag="ISB30_000565"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP1988244.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            21841..22566
                     /gene="rpsB"
                     /locus_tag="ISB30_000566"
     CDS             21841..22566
                     /gene="rpsB"
                     /locus_tag="ISB30_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP1988245.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            22701..23552
                     /gene="tsf"
                     /locus_tag="ISB30_000567"
     CDS             22701..23552
                     /gene="tsf"
                     /locus_tag="ISB30_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP1988246.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            23699..24424
                     /gene="pyrH"
                     /locus_tag="ISB30_000568"
     CDS             23699..24424
                     /gene="pyrH"
                     /locus_tag="ISB30_000568"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP1988247.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            24574..25131
                     /gene="frr"
                     /locus_tag="ISB30_000569"
     CDS             24574..25131
                     /gene="frr"
                     /locus_tag="ISB30_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP1988248.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            25223..26419
                     /gene="ispC"
                     /locus_tag="ISB30_000570"
     CDS             25223..26419
                     /gene="ispC"
                     /locus_tag="ISB30_000570"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAP1988249.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALKDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            26608..27366
                     /gene="ispU"
                     /locus_tag="ISB30_000571"
                     /gene_synonym="uppS"
     CDS             26608..27366
                     /gene="ispU"
                     /locus_tag="ISB30_000571"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAP1988250.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            27379..28236
                     /gene="cdsA"
                     /locus_tag="ISB30_000572"
     CDS             27379..28236
                     /gene="cdsA"
                     /locus_tag="ISB30_000572"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP1988251.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLVTAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            28248..29600
                     /gene="rseP"
                     /locus_tag="ISB30_000573"
     CDS             28248..29600
                     /gene="rseP"
                     /locus_tag="ISB30_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAP1988252.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            29630..32062
                     /gene="bamA"
                     /locus_tag="ISB30_000574"
     CDS             29630..32062
                     /gene="bamA"
                     /locus_tag="ISB30_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAP1988253.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            32184..32669
                     /gene="skp"
                     /locus_tag="ISB30_000575"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             32184..32669
                     /gene="skp"
                     /locus_tag="ISB30_000575"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAP1988254.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            32673..33698
                     /gene="lpxD"
                     /locus_tag="ISB30_000576"
     CDS             32673..33698
                     /gene="lpxD"
                     /locus_tag="ISB30_000576"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAP1988255.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            33803..34258
                     /gene="fabZ"
                     /locus_tag="ISB30_000577"
     CDS             33803..34258
                     /gene="fabZ"
                     /locus_tag="ISB30_000577"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP1988256.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            34262..35050
                     /gene="lpxA"
                     /locus_tag="ISB30_000578"
     CDS             34262..35050
                     /gene="lpxA"
                     /locus_tag="ISB30_000578"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAP1988257.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            35050..36198
                     /gene="lpxB"
                     /locus_tag="ISB30_000579"
     CDS             35050..36198
                     /gene="lpxB"
                     /locus_tag="ISB30_000579"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAP1988258.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            36195..36791
                     /gene="rnhB"
                     /locus_tag="ISB30_000580"
     CDS             36195..36791
                     /gene="rnhB"
                     /locus_tag="ISB30_000580"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP1988259.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            36828..40310
                     /gene="dnaE"
                     /locus_tag="ISB30_000581"
     CDS             36828..40310
                     /gene="dnaE"
                     /locus_tag="ISB30_000581"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP1988260.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAESRGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            40323..41282
                     /gene="accA"
                     /locus_tag="ISB30_000582"
     CDS             40323..41282
                     /gene="accA"
                     /locus_tag="ISB30_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP1988261.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            41380..43521
                     /gene="ldcC"
                     /locus_tag="ISB30_000583"
     CDS             41380..43521
                     /gene="ldcC"
                     /locus_tag="ISB30_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAP1988262.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDSWF
                     FDIWQPPQVDEAECWHVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            43578..43967
                     /locus_tag="ISB30_000584"
     CDS             43578..43967
                     /locus_tag="ISB30_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP1988263.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            44032..45345
                     /gene="tilS"
                     /locus_tag="ISB30_000585"
     CDS             44032..45345
                     /gene="tilS"
                     /locus_tag="ISB30_000585"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP1988264.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWARQYDLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTQQGTLKIAPMLAMSDARRAAIIRRWLAGQNAPMPSREALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(45379..45639)
                     /gene="rof"
                     /locus_tag="ISB30_000586"
     CDS             complement(45379..45639)
                     /gene="rof"
                     /locus_tag="ISB30_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAP1988265.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(45626..45826)
                     /locus_tag="ISB30_000587"
     CDS             complement(45626..45826)
                     /locus_tag="ISB30_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAP1988266.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            45992..46537
                     /locus_tag="ISB30_000588"
     CDS             45992..46537
                     /locus_tag="ISB30_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAP1988267.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            46534..46956
                     /gene="arfB"
                     /locus_tag="ISB30_000589"
     CDS             46534..46956
                     /gene="arfB"
                     /locus_tag="ISB30_000589"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP1988268.1"
                     /translation="MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            46970..47680
                     /gene="nlpE"
                     /locus_tag="ISB30_000590"
                     /gene_synonym="cutF"
     CDS             46970..47680
                     /gene="nlpE"
                     /locus_tag="ISB30_000590"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAP1988269.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAKSSMPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNGEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(47836..48660)
                     /locus_tag="ISB30_000591"
     CDS             complement(47836..48660)
                     /locus_tag="ISB30_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAP1988270.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGNNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSNVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRSGDVSAFKPETQLQYIGHLKPGIYIKASRFVGLTQ"
     gene            complement(48713..50431)
                     /gene="proS"
                     /locus_tag="ISB30_000592"
     CDS             complement(48713..50431)
                     /gene="proS"
                     /locus_tag="ISB30_000592"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP1988271.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(50542..51249)
                     /gene="tsaA"
                     /locus_tag="ISB30_000593"
     CDS             complement(50542..51249)
                     /gene="tsaA"
                     /locus_tag="ISB30_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAP1988272.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(51246..51650)
                     /gene="rcsF"
                     /locus_tag="ISB30_000594"
     CDS             complement(51246..51650)
                     /gene="rcsF"
                     /locus_tag="ISB30_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAP1988273.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(51768..52583)
                     /gene="metQ"
                     /locus_tag="ISB30_000595"
     CDS             complement(51768..52583)
                     /gene="metQ"
                     /locus_tag="ISB30_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAP1988274.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(52623..53276)
                     /gene="metI"
                     /locus_tag="ISB30_000596"
     CDS             complement(52623..53276)
                     /gene="metI"
                     /locus_tag="ISB30_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAP1988275.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTISAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(53269..54300)
                     /gene="metN"
                     /locus_tag="ISB30_000597"
     CDS             complement(53269..54300)
                     /gene="metN"
                     /locus_tag="ISB30_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAP1988276.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            54488..55060
                     /gene="gmhB"
                     /locus_tag="ISB30_000598"
     CDS             54488..55060
                     /gene="gmhB"
                     /locus_tag="ISB30_000598"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAP1988277.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        13032 a        13567 c        15099 g        13420 t
ORIGIN      
        1 aaatgttact attccgttaa aagcacgcta catcaaaatg ggtagcgtca aaagtggtcg
       61 ggttaattct gttgccacct tcgaagtgta ttaccgataa gcttttccct ttgccataaa
      121 tgttaatttt tggtaatgga atttttatgg tttacggtgc aaagattaac atttttgatt
      181 catctgattg attctcgccc cttcatggac gcacagagcg tatatcactt gcgaacccgc
      241 cagcgtttcg aatattatcg catctttata ataatcattc tcgtttacgt tatcattcac
      301 tttcatcaga gatataccaa tggcgcgttc caaaactgct cagccaaaac actcactgcg
      361 taaaatcgca gttgtagtag ccacagcggt tagcggcatg tctgtttatg cacaggcagc
      421 ggttgaaccg aaagaagaca ctatcaccgt taccgctgca cctgcgccgc aagaaagcgc
      481 atgggggcca gctgcaacta ttgcggcgcg acagtccgct accggcacta aaactgatac
      541 gccgattcaa aaagtacctc aatctatttc tgtcgtcacc gctgaagaga tggcactgca
      601 tcagcctaag tcggtgaaag aagcgcttag ctatacccct ggcgttgctg tgggaacccg
      661 tggcgcatct aatacttatg attacctgat catccgcgga tttgcggcag acggccaaag
      721 ccagaacaac tatctgaatg gcctgaagat gcagggcaac ttctataacg atgcggttat
      781 tgacccatat atgctggagc gcgctgaaat catgcgtggc ccggtttccg tgctgtatgg
      841 gaaaagcagc ccaggcggcc tgttgaacat ggtcagcaag cgcccgacca ctgaaccgtt
      901 gaaagaagtt cagtttaagg ccgggactga cagcctattc cagactggtt ttgacttcag
      961 cgatgcgctg gatgatgacg gcgtttactc ttatcgcctg accggccttg cgcgttctgc
     1021 gaatgcccag cagaaggggg ctgaagagca gcgttatgct attgctccag cgtttacctg
     1081 gcgtccgaat gataaaacca atttcacttt cctttcttac ttccagaacg agccggaaac
     1141 cggttattac ggctggttgc cgaaagaggg gactgtcgaa ccgctgccaa acggtaagcg
     1201 tctgccgacc gactttaacg aaggcgcgaa gaacaacacc tattctcgta acgagaagat
     1261 gattggttat agcttcgacc acgaatttaa cgacaccttt actgtgcgtc agaacctgcg
     1321 ctttgcacaa aataaagtct cgcaaaagag cgtatatggc tacggcatgt gctcagaccc
     1381 gctctattct tcaaacccct cttcgagtcc atgtgctaac gtgccgcagt ctcaatgggg
     1441 ccatacactg actcgtcagt acgttatcga taatgagaag ttagaaaact tctctgttga
     1501 tactcagttg caaagcaaat tcgcaaccgg ttcagtggat cacactctgc tgactggcgt
     1561 tgactttatg cgtatgcgta atgacatcga ttcctggttt ggttatgccg gttcagttgc
     1621 accatctgat atctataatt tagaccgtag tgactttgat tttggcgctc acccgaatcc
     1681 gtctggtcca taccgcgttc tgctgaaaca gaaacaaacc ggcctgtatg ttcaggatca
     1741 ggcgcagtgg gataaagtgc tggttacgct tggcggtcgc tatgactggg ccgatcagtc
     1801 atctttcaac cgtgattacg gtaataaatc tgaacgtgac gacaaagagt tcacctggcg
     1861 tggtggtgta aactacctgt tcgacaacgg cgtaacgcct tacttcagct acagtgaatc
     1921 atttgaaccc gcttcacaga ctgatgcgaa cggcgatctg tttgcacctt cgaaaggcaa
     1981 acagtatgaa gtcggcgtga aatatgtgcc agaagatcgc ccaattgtgg tgactggcgc
     2041 actgtatcag cttaccaaaa ctaataacct gatggcggat ccgaacggtt cactgttctc
     2101 ggttgaaggc ggtgagattc gtgcgcgtgg cgtagagctg gaagcgaaag cggcactgtc
     2161 ggcgagtgtt aacttggttg gttcttacac ctatactgac gcggaataca ccactgacac
     2221 caactacaaa ggaaatacgc ctgcacaggt gccaaaacac atggcttcgc tgtgggctga
     2281 ctataccttc tttgacggtc cgctttcagg tctgacgctg ggcaccggtg gtcgttatac
     2341 tggctccagc tatggtgatc cggctaactc ctttaaagtg ggaagttata cggtcgtgga
     2401 tgcgttagtg cgttatgatc tggcgcgagt cggcatggcg ggctccaacg tggcgctgca
     2461 tgtcaacaac ctgttcgatc gtgaatacgt cgccagctgc tttaacactt atggttgctt
     2521 ctggggcgca gaacgtcagg tcgttgcaac cgcaaccttc cgtttctaat ttctcttttt
     2581 ggggcacgga tttccgtgcc cgtttcacaa gttggctgtt atgcaggaat acacgaatca
     2641 ttccgatacc acttttgcac tgcgtaatat ctcctttcgt gtgcccgggc gcacgctttt
     2701 gcatccgctg tcgttaacct ttcctgccgg gaaagtgacc ggtctgattg gtcacaacgg
     2761 ttctggtaaa tccactctgc tcaaaatgct tggtcgtcac cagccaccgt cggaagggga
     2821 gattcttatt gatgcccaac cgctggaaag ctggagcagc aaagcgtttg cccgcaaagt
     2881 ggcttatttg ccgcagcagc ttcctccggc agaagggatg accgtgcgtg aactggtggc
     2941 gattggtcgt tacccgtggc atggcgcgct ggggcgcttt ggtgttgcag atcgcgaaaa
     3001 ggtcgaggaa gctatctcgc tggttggctt aaaaccgctg gcgcatcggc tggtcgatag
     3061 cctttctggc ggcgaacgtc agcgggcgtg gatcgccatg ctggtggcgc aggatagccg
     3121 ctgtctgttg ctcgacgaac cgacttcggc gctggatatc gcccaccagg ttgatgtact
     3181 ggcgctggtg catcgtttaa gtcaggagcg tggcctgacg gtcattgccg tgctgcacga
     3241 tatcaatatg gcggcgcgct actgtgatta tctggtcgcc ctacgcggcg gtgaaatgat
     3301 tgctcaggga acgcctgcgg aaattatgcg cggcgaaacc ctcgaaatga tttatggcat
     3361 cccgatgggt attctgccgc atccggcggg tgctgcacct gtgagttttg tttattgatg
     3421 agcggcttac ctcttatttc gcgccgccga ctgttaacgg cgatggcgct ttctccgttg
     3481 ttatggcaga tgaataccgc ccacgcggcg gttattgatc ccaatcgtat tgtggcactg
     3541 gagtggttgc cggtggagtt actgctggcg ctcggcatcg tgccttacgg cgtggcggac
     3601 accatcaact atcgcctgtg ggtcagcgaa ccgccattgc cggactcagt gattgacgtt
     3661 ggtttgcgta cagaacccaa ccttgaactg ctgaccgaaa tgaaaccgtc gtttatggtc
     3721 tggtctgcag gatatggccc ttcaccagaa atgctggcac gtattgcgcc ggggcgcgga
     3781 tttaacttca gcgacggcaa acagccactg gcgatggcgc gtaaatcact gacggaaatg
     3841 gcagatttac ttaacctgca aagcgcagcg gaaacgcatt tagcgcacta tgaagacttt
     3901 atccgcagca tgaaaccccg ctttgtgaag cgtggtgcac gcccgttatt gctgacgacg
     3961 cttatcgatc cgcgccatat gttggtcttc ggtccaaaca gcttgttcca ggaaattctc
     4021 gatgagtacg gcatcccaaa tgcctggcaa ggggaaacca acttctgggg cagtaccgcc
     4081 gtcagtatcg atcgtctggc agcgtataaa gacgttgatg tgctctgttt tgatcacgac
     4141 aacagcaaag acatggatgc gctaatggca accccgctgt ggcaggccat gccgtttgtc
     4201 cgcgccggac gctttcagcg cgtacctgca gtttggttct atggtgcgac gctttctgca
     4261 atgcactttg tgcgtattct ggataacgcc atcggaggta aagcgtgagt aaacgaattg
     4321 cgctttttcc ggtgttattg ctggcgctgc tggtggttgc tgctgcggcg ttgacctgga
     4381 tgaacttctc gcaggcgctg ccgcgcagcc agtgggcgca ggccgcctgg tcgccggata
     4441 ttgacgtcat cgagcagatg atttttcact acagcttgtt gccgcgtctg gcgatttcgc
     4501 tgctggtggg cgcgggcctg gggatggtgg gcgtgctgtt tcagcaagtg ctgcgtaacc
     4561 cgctggcgga accgacgacg ttgggtgttg caacaggcgc gcaactgggg attaccgtca
     4621 ccacgctctg ggcgatcccc ggcgcgatgg cgagccagtt tgctgcgctg gcaggggctt
     4681 gtgttgttgg cttaatcgtc tttggcgtcg cgtgggggaa acggctttcg ccggtaacgc
     4741 tgatcctcgc ggggctggta gtgagccttt attgcggcgc aatcaatcag ttactggtta
     4801 tcttccatca tgaccaactg caaagcatgt tcctgtggag caccggaacg ctgacgcaaa
     4861 ccgactgggg cggcgttgag cgtttatggc cgcagctgct gggcggcgtg atgctgacgt
     4921 tattgctact tcgcccgtta actctgatgg ggcttgatga tggcgtggcg cgcaatctcg
     4981 ggctggcctt gtcgctcgct cgtctggcgg cgttgtcgct ggcgattgtc atcagtgcgc
     5041 tgctggtgaa cgcggtgggg attatcggct ttatcggttt gttcgcgcca ctgctggcga
     5101 aaatgctggg ggcgcggcgt ttgttgccac ggttgatgct ggcatcgctg attggtgcgt
     5161 taattctgtg gctttctgat caaatcatcc tctggctgac tcgcgtgtgg atggaagtgt
     5221 ctaccggttc ggtcactgcg ttgatcggtg cgccgctgct actgtggctg ctgccgcgtt
     5281 tacgcagcat tagcgcgccg gatatgaagg tcaacgatcg tgtcgcgact gaacgccaac
     5341 acgtgctggc gtttgctctc gcgggcggcg tgctgctgtt gatggctgtg gtggtggcgc
     5401 tgtcgtttgg tcgtgatgcg cacggctgga cgtgggcgag cggggcgttg ctcgaggatt
     5461 tgatgccctg gcgctggccg cgaattatgg cggcgctgtt tgcgggtgtc atgctggcgg
     5521 tggcgggctg tattattcag cggctgaccg gaaacccgat ggcaagcccg gaagtgctgg
     5581 ggattagctc cggcgcggcg tttggcgtgg tgttaatgct gtttctggtg ccgggtaatg
     5641 cctttggctg gctgttaccc acaggcagtc tcggggcggc ggtgacgctg ttgatcatta
     5701 tgatcgccgc cggacgcggt ggattttccc cacaccgcat gttactggcg gggatggcgt
     5761 taagtaccgc gttcaccatg cttttgatga tgttgcaggc aagtggtgac ccgcgaatgg
     5821 cgcaagttct gacctggatt tccggttcga cctacaacgc gaccgatgcg caggtctggc
     5881 gtactggaat tgtgatggtg attttgctgg cgattacccc gctgtgccgc cgctggctga
     5941 ccattttacc gctgggtggt gataccgcac gagccgtggg gatggcgctg acgccgacgc
     6001 gaattgcgct gctgctgtta gcggcttgcc tgacggcgac cgcgacaatg accattggtc
     6061 cgttgagttt tgttggttta atggcaccgc atattgcgcg gatgatgggc tttcgacgga
     6121 cgatgccaca catcgtaatt tcggcgctgg tgggaggatt actgctggtg ttcgctgact
     6181 ggtgtgggcg gatggtattg tttccattcc agatcccagc ggggctgctg tcgaccttta
     6241 tcggcgcgcc atattttatc tatttgttga gaaagcagag ccgttaatag gatgcctcgc
     6301 ccggtgggcg aggcaaaatc atcacaactt cgcaaacacc cgacgtgcag catcgatggt
     6361 gttattgata tcttccatgc tgtgcgccac agacataaag cccgcttcaa acgctgacgg
     6421 tgccagataa acaccttcgt ccagcatcat atggaagaaa cgtttaaagc gttccacgtc
     6481 acaggacatc acatcctggt agcatgtcac ggactcggcg tcggtaaaga aaataccgaa
     6541 catgccgcca acgtggttta ccaccagcgg aattccggct tcttctgccg cttcccgcag
     6601 accttctgcc agacgtgttg tcagctcatc cagcgtctcg tgaacgcccg gctgtgcgac
     6661 ttcattcaga caggcgaaac cagcagccat cgcaatcggg ttaccggaaa gcgtacccgc
     6721 ctgatagacc ggaccggtcg gggccagcgc atccattaca tcacgacgac caccgaatgc
     6781 gcctaccggc attccaccgc cgatgatttt gccgaggcag gtgagatccg gttccacacc
     6841 gtaataatcc tgtgcgccag ctagcgctac gcggaaaccg gtcatcactt catcgatgat
     6901 cagcaacgcg ccaaattcgt cgcacagcgc gcgcagacct ggcaggaact ctggcagcgg
     6961 tggcacgcag ttcatattgc ctgccaccgg ctcgacgata atacaggcaa tctcttgcgg
     7021 gtattgctca aacgcggcgc gtacagaggc cagatcgtta taagtacagg ttaaggtatg
     7081 tttggcgaaa tctgccggaa cacccggcga atttggctgg cctaacgtga gtgcgccaga
     7141 accggctttc accagcaagc agtcagcgtg accgtggtaa caaccttcaa atttaataat
     7201 tttgtcgcga ccggtaaaac cacgggccag gcggatggcg ctcatcgtcg cctcggtgcc
     7261 ggagttcacc atgcgcacca tatccatggt cgggaccagt tcggtcacca gttgcgccat
     7321 tttcacttcc atttcggttg gtgcaccaaa gcttaaacca cgctcggcgg cttcaatcac
     7381 ggcattgcgg atcgccggat ggttatggcc cagcaccatc ggcccccagg aaccgacata
     7441 atcgatatag gctttgccat caacatcgta cagataagcg ccgtccgctt tttcgataaa
     7501 cagtggagtg ccgcccacgc cagtaaaggc gcgaacaggg gagttcacgc cgccagggat
     7561 cagctcgcgc gctgcgctgt aaagattttc agacttactc atggagggtt cctgattcgt
     7621 agaaaaagtg aatggctgct attctatgtt attcataaca agttaaatac tcgtcaaaca
     7681 tcaggctgct tgtactggtg caatcctgat ttcttagagt ataaaagttt tgtgcatttg
     7741 aaacattacg ctttgcaaag gattttcatg gaacgtgcga gtaaaatgcc gtcatcttat
     7801 ttgtatgacc aataagtgat cattggatga aaactgatac tccctcttta gaaacaccgc
     7861 aggccgcgcg cctgcgacgc agacaactga ttcgccaact tcttgagcgc gataaaaccc
     7921 cgttagccat tttgtttatg gcggcagtcg tcggcacgct tgtcgggctg gctgcggttg
     7981 cttttgacaa aggtgtcgcc tggttgcaga accaacgtat gggggcactg gtacatactg
     8041 ctgataatta tccgcttctg ttaaccgtcg cttttctctg ttcggcggtg ctggcgatgt
     8101 tcggctactt tttggtgcgc aaatacgcgc cggaagcagg tggttcgggg atcccggaaa
     8161 ttgaaggggc gctggaagat caacgtcctg ttcgctggtg gcgtgtgttg ccggtgaaat
     8221 tctttggtgg gctggggaca ctcggcggag gcatggtgtt ggggcgcgaa gggccaaccg
     8281 tgcagatagg cggcaatatc gggcggatgg tactcgatgt tttccgcctg aaaggtgacg
     8341 aagctcgtca tacgctactg gcaaccggtg ctgctgcggg gctggctgcg gcctttaacg
     8401 cgccgctggc gggtattttg tttattatcg aagagatgcg tccgcagttt cgctatacgt
     8461 taatttcgat taaagcggta tttattggcg tcattatgtc gaccattatg taccggattt
     8521 ttaatcatga agttgcgttg attgacgtcg gtaaactttc tgatgcgccg cttaatacgc
     8581 tgtggcttta tctgatcctc ggtattattt ttggcatttt cggccctatt tttaataaat
     8641 gggtgctggg gatgcaggat ttgctgcacc gtgtgcacgg tggcaatatt accaaatggg
     8701 tactgatggg cggtgcgatt ggcggtttgt gtggattgct ggggtttgtg gcaccagcaa
     8761 cttcgggcgg cggttttaac ctgattccta tcgctaccgc ggggaatttc agcatgggaa
     8821 tgctggtgtt tatcttcgtc gcgcgggtca ttaccacctt actctgcttc tcttccggcg
     8881 cgccgggcgg tatttttgcc ccgatgctgg cgctgggtac tgtgctggga accgctttcg
     8941 gaatggttgc cgttgagttg tttccgcaat atcaccttga ggcggggacg tttgctattg
     9001 ccggaatggg ggcgttactg gcggcatcta ttcgcgcgcc gttaacgggg atcattctgg
     9061 ttctggaaat gaccgataac taccagctca ttttgccaat gattattacc ggtcttggcg
     9121 caacactatt agcacaattt accggtggga aaccgctata ctcggcgatt cttgcgcgca
     9181 cgctggcaaa acaggaagct gagcaactgg cgcgaagcaa ggccgcatca gccagcgaga
     9241 atacttgaac gaaataccag ggtattagat aatggcgatt attattgggt tagaatttgc
     9301 ccaattgccg atgtcgtttg gagcaaaata tgagtgatga cgtagcactg ccgctggagt
     9361 ttaccgacgc agcagccaac aaagttaaaa gcctgatcgc tgacgaagat aacccgaatc
     9421 tgaaattacg cgtgtatatc accggtggcg gttgcagcgg cttccagtat ggtttcacct
     9481 ttgatgatca ggtgaacgaa ggcgatatga ccatcgaaaa acagggcgtt ggcctggtgg
     9541 ttgatccgat gagcctgcaa tatctggtcg gcggttccgt tgactatacc gaaggtctgg
     9601 aaggttctcg tttcatcgtg accaacccga acgcgaaaag cacctgcggt tgcggttctt
     9661 cctttagtat ctaatctgct gttcatgtgc cggataaact atccggcaca tctcctctct
     9721 tcaacgacca ttctcatcca gcgcaaacgt cggtagctta agatgccaac gaatagccgc
     9781 cagccgaatc aatagcgtca cgaccatgcc catcatactg gctgtttcca gtggtacgga
     9841 aaatgtgtaa taagccgtag cgtggacaat accgccgata atacaggcag ttgcgtagat
     9901 ttctgtacgt aaaatcatgg ggatttcgcg ggccagtaca tcacgaatga ttccgccgcc
     9961 aacgccagta atgacgccca tacaaaccgc gattaacgga ccggcttccg cattaaaggc
    10021 tttattcacg ccaatgccga caaacaccgc cagaccaacg gcgtccagca ccggcaacat
    10081 ccattttggt aagcgtcttg gctggcgcac cagcacgatg gtcagcatgc tggtgaccat
    10141 tgcaacgacc agatcggtgg gatctttcac ccaaaatacc gggccgtgat ccagcgccat
    10201 gtcgcgaatt gttccgccgc ctactgcggt aaccacgccc aataccagaa caccaaaagg
    10261 gtccatacgc aattttccgg ctaacaaaac gccagagata gcaaataccg ctgtgccgac
    10321 tatatccagc caatagacga gcattgttca atccccactg agcacctgaa aaggtcaggc
    10381 actaatctac ctgtgaaagc gcattacaga gctgttgtgc agcgaggata atacgtgggc
    10441 ttgcacgttc aaaccagtca ctcgtgagag gaataacggg aattttgagc tgttcacccc
    10501 agtattgttt gattttagga atttggtccg gtccgcctgt aatgacaatc gcctgtggcg
    10561 agcgtgctaa cacctgttcg cggctaactt gcggccaggg aacccggctg tctttaaaga
    10621 tgttttctcc gccacaaact tcgagtacct ggttctgaat cgactctttt ccactggtaa
    10681 atggcggatt aatgccgaat tgcagaaaaa cacgtttttt aggtttatca gcatattgcg
    10741 ctttcaattg cgcgtactga tccagcaggg attgcgcggc ttgttcggcc ttatccggtt
    10801 gcggactcca gggggccagt tgacgtaacg cattggcaat ttgttcaatg cttgtcgcat
    10861 cgacccacat cacttttatt cccagcgaag ccagctggtc aacctgccgc tcggcattac
    10921 ctccacgcca ggcaatcacc agatcgggtt tcagcgcgac aatgcgttcc agattcatcc
    10981 cctgccaggt ggaaacctgc tcaatctttt gcgcttgtgg aggatagtcg gaatagctgc
    11041 tgaccccaac cggcgtgatc ccggcggcaa aggcaagttc agtgttggcg ggagaaagcg
    11101 tgatgacgcg cggcgcggca ttgagccaca gcggcgcgag aaaagacagg gcgaccagcg
    11161 ccctgaacag tgacttagcc atgtgccagt ttctgcacca gtgactcaac catcaggctg
    11221 gactgtttag cggcaacagc caggaactca tcgaagctaa gatgagactg ttgatcggcc
    11281 acgtcggaga tggcgcgtac tacgacaaac gggacgttga aattgtggca gacatgggcg
    11341 attgccgtcg cttccatctc tacagcaatg gcctgtggga agttgtggcg gattttcgcc
    11401 agaccaacag aaccgttgat gaaagcgtcg ccgctaacaa tcaggccacg tacagcgtta
    11461 agattcagtt cggcaatgca ggcctcagcg gcagcgatca gtttatcgtc agctttgaag
    11521 cctgccgggc agcctggtaa ctgaccgtat tcataaccaa atgccgtgac atccgcgtcg
    11581 tgataacgcg cttcgtccga gacaacgata tcgcccactt tcagcgttgg tgccaggcca
    11641 ccggcagaac cggtgttaat aatcacatct ggcttgcagt gttccaacag caaagtggca
    11701 cccagcgccg cagcgacttt accgatgccc gatttcagaa gcgcaacctc ggttccattc
    11761 agttggccgg tatagatttc gcaaccgccc agactgatag tttgacggtt ttcgattttg
    11821 tcacgcagca gcgtaacttc ttcttccatt gcaccaatga tgccgatttt catagattta
    11881 ctcgcgataa gcccgatttg aaggcatagt ttaccatgcg cttacgggga agcgtatttc
    11941 tcacgcggga gaggacatgg cacagattga tttccgaaaa aaaataaact ggcatcgtcg
    12001 ttaccgttca ccgcagggcg ttaaaaccga acatgagatt ctgcggatct tcgagagcga
    12061 tcgcgggcgt atcatcaact ctccggcaat tcgtcgtctg caacaaaaga cccaggtttt
    12121 tccactggag cgcaatgccg ccgtgcgcac gcgtcttacc cactcgatgg aagtccagca
    12181 ggtggggcgt tatatcgcta aagagatttt aagccgcctg aaagagctta aattactgga
    12241 gacgtatggt ctcgatgaac tgaccgggcc ctttgaaagc attgttgaga tgtcatgcct
    12301 gatgcacgat atcggcaatc cgccgtttgg tcattttggc gaagcggcga taaatgactg
    12361 gtttcgccag cgtttgtacc cggaagatgc cgaaagccag cctctgactg acgatcgctg
    12421 cagcgtggcg gcactacgtt tacgggacgg ggaagaaccg cttaacgcgc tgcggcgcaa
    12481 gattcgtcag gacttatgtc attttgaagg taacgcacag ggtattcgtt tggtgcatac
    12541 attgatgcgg atgaatctca cctgggcgca ggttggcggt attttaaaat atacccgtcc
    12601 ggcgtggtgg cgtggtgaaa cgcctgagac acatcactat ttaatgaaaa agccgggcta
    12661 ttatctttcc gaagaagcct atattgcccg gttgcgtaaa gaacttaatt tggcgcttta
    12721 cagtcgtttt ccgttaacgt ggattatgga agctgccgac gacatctcct attgtgtggc
    12781 agaccttgaa gatgcggtag agaaaaggat atttaccgtt gagcagcttt atcatcattt
    12841 gcacgaggcg tggggccagc atgagaaagg ttcgctcttt tcgctggtgg ttgaaaatgc
    12901 ctgggaaaaa tcacgctcaa atagtttaag ccgcagtacg gaagatcagt tttttatgta
    12961 tttacgggta aacaccctaa ataaactggt accctacgcg gcacaacgat ttattgataa
    13021 tctgcctgcg attttcgccg gaacgtttaa tcatgcatta ttggaagatg ccagcgaatg
    13081 cagcgatctt cttaagctat ataaaaatgt cgctgtaaaa catgtgttta gccatccaga
    13141 tgtcgagcag cttgaattgc agggctatcg ggtcattagc ggattattag agatttatcg
    13201 tcctttatta aacctgccgt tatcagactt tactgaactg gtagaaaaag aacgggtgaa
    13261 acgtttccct attgaaacgc gcttattcca caaactctcg acgcgccatc ggctggccta
    13321 tgtcgaggct gtcagtaaat taccgtcaga ttctcctgag tttccgctat gggaatatta
    13381 ttaccgttgc cgcctgctgc aggattatat cagcggtatg accgacctct atgcgtggga
    13441 tgaataccga cgtctgatgg ccgtagaaca ataaccaggc ttttgtaaag acgaacaata
    13501 aatttttacc ttttgcagaa actttagttc ggaacttcgc gctataaaac gaatctgaag
    13561 aacacagcaa ttttgcgtta tctgttaatc gagactgaaa tacatgaaaa aaaccacatt
    13621 agcactgagt gcactggctc tgagtttagg tttggcgtta tctccgctct ctgcaacggc
    13681 ggctgagact tcttcagcaa cgacagccca gcagatgcca agccttgcac cgatgctcga
    13741 aaaggtgatg ccttcagtgg tcagcattaa cgtagaaggt agcacaaccg ttaatacgcc
    13801 gcgtatgccg cgtaatttcc agcagttctt cggtgatgat tctccgttct gccaggaggg
    13861 ttctccgttc cagagctctc cgttctgcca ggggggcccg ggcggtaatg gcggcggcca
    13921 gcaacagaaa ttcatggcgc tgggttccgg cgtcatcatt gatgccgata aaggctatgt
    13981 cgtcaccaac aaccacgttg ttgataacgc gacggtgatt aaagtccagc ttagcgatgg
    14041 tcgtaagttc gacgcgaaga tggttggcaa agatccgcgc tctgatatcg cgttgatcca
    14101 gatccagaac ccgaaaaacc tgaccgcaat taagatggcg gattctgatg cgctgcgcgt
    14161 gggtgattac accgtagcaa ttggtaaccc gttcggcctg ggcgagacag taacttccgg
    14221 gattgtctct gcgctgggac gtagcggcct gaacgctgaa aactacgaaa acttcatcca
    14281 gaccgatgca gcgatcaacc ggggtaactc aggtggtgcg ttggttaacc tgaacggcga
    14341 actgattggt atcaacaccg cgatcctcgc accggacggc ggaaacatcg gtatcggttt
    14401 tgctatcccg agcaacatgg tgaaaaacct gacctcgcag atggtggaat acggccaggt
    14461 gaaacgtggt gagttgggta ttatgggcac tgagctgaac tccgatctgg cgaaagcgat
    14521 gaaagttgac gctcagcgcg gtgctttcgt aagccaggtt ctgccgaatt cttccgccgc
    14581 gaaagcgggc attaaagcgg gtgatgtgat cacctcactg aacggtaagc caatcagcag
    14641 ctttgccgca ctgcgtgctc aggtgggcac tatgccggta ggtagcaaac tgaccctggg
    14701 cttactgcgc gacgggaagc aggttaacgt gaacctggaa cttcagcaga gcagccagaa
    14761 tcaggttgat tccagcacca tcttcaacgg cattgaaggc gctgagatga gtaacaaagg
    14821 caaagatcag ggcgtggtgg tgaacaacgt gaaaacgggg actccggctg cgcagatcgg
    14881 cctgaagaaa ggtgatgtga ttattggcgc gaaccagcag gcagtgaaaa acatcgctga
    14941 actgcgtaaa gttctcgaca gcaaaccgtc tgtgctggca ctgaacattc agcgcggcga
    15001 cagcactatc tacctgttaa tgcagtaatc tccctcaacc ccttcctgaa aacgggaagg
    15061 ggttctcctt acaatctgtg aacttcacca caactccata catcttcatc atcctttggg
    15121 catttgcaca atgccgtacg ttacgtactt ccttatgcta agccgtgcat aatggaggac
    15181 ttatggctgg ttggcatctt gataccaaaa tggcgcagga tattgtggca cgtaccatgc
    15241 gcatcatcga taccaatatc aacgtaatgg atgcccgcgg gcgaattatc ggcagcggcg
    15301 atcgtgagcg tattggtgaa ttgcacgaag gagcattgct ggtactttca caaggacgag
    15361 tcgtcgatat cgatgacgcg gtggcacgtc atctgcacgg tgtgcggcag gggattaatc
    15421 taccgttacg gctggaaggt gaaattgtcg gcgtaattgg cctgacaggt gaaccagaga
    15481 atctgcgtaa atatggcgaa ctggtctgca tgacggctga aatgatgctg gaacagtcgc
    15541 ggttgatgca cctgttggcg caggatagcc gtttgcggga agaactggtg atgaacctga
    15601 ttcaggctga ggagaatact cccgcactta ctgaatgggc gcaacggctg gggatcgatc
    15661 tcaatcaacc gcgagtggtg gctattgttg aggtcgacag cggtcagctt ggtgtggaca
    15721 gcgcaatggc ggagttacag caactgcaaa atgcgctgac tacgcccgag cgtaataatc
    15781 tagtggcgat tgtctcgcta accgaaatgg tggtgttgaa accggcgttg aactcttttg
    15841 ggcgctggga cgcagaagat catcgtaagc gagttgaaca actgattacc cgcatgaaag
    15901 agtatggcca gctgcgtttt cgcgtttcgc tgggcaacta ttttaccggt cctggcagta
    15961 ttgcccgatc ctatcgtacg gcgaaaacga cgatggtggt gggtaaacag cggatgccag
    16021 aaagtcgctg ctatttttat caggatctga tgttacctgt gttactcgac agtttgcgtg
    16081 gcgactggca ggccaacgaa ctggcgcgac cgctggcgcg gctgaaagcg atggacaata
    16141 acggcttgct gcggcgaact ctggcggcgt ggtttcgcca caatgtgcaa ccgctggcaa
    16201 cgtcaaaggc gttgtttatt catcgtaata ctctggaata tcggcttaat cgtatatcgg
    16261 aactgaccgg gcttgatttg ggcaattttg atgacaggtt gctgctgtat gtggcgttac
    16321 aactggatga agagcggtag gtcgattaca aaattacagc cgggtgagac tttacccggc
    16381 ccacagaatg acattattta cgcgtcagtt tttccagatc ggcttcaatc tcgctgatct
    16441 tattggttac tactgactcc aggtgacgta agtcatcgag gatcttacgc ttaagatcaa
    16501 cttcgctgcg gtcacgctgg cagatttgat caagctcatc aatgatatac cgtagattcg
    16561 ggctgatttc ctggacttct ttataaccct gccccacacc atcagcgaca accgttttac
    16621 gctgacgcgg atatttaaac ttcacgctct tggcgaaaaa ctcgcctttg tctttctgga
    16681 aatagatttt cagaatatcg ttgttggctt cctgccggag gctgtaacga tcaatttctt
    16741 caggattggt aatacccaga cttttcagat tgtcgtacat agcgttacct caaaatgagt
    16801 cagtaaattg tgcttatttt agcatttggc ctggcccgcc cggctcgact gtgatcgcag
    16861 aaagcctttt tcttccagac ttctatcatg gcgtaaaaat caaaaaatta cctgctttat
    16921 tgtgatgata ttccatggga tggattagca catttatctt cattttgcga attacatgac
    16981 agtccgaaac aagatgaaaa cgtatttaaa aaagggggaa aatgagtagg acgaattatt
    17041 ctgagccaga aagcaatcgg gcagcgtcgt gctgcccgtg tacatacttt tagtcgatgg
    17101 tacgcagcag ttcgttaata ccaactttac cgcgagtttt cgcgtcaact ttcttaacga
    17161 taactgcaca gtagagactg tatttgccat ctttcgacgg cagattacct gaaacaacca
    17221 cagaccctgc cggaacgcga ccgtagtgga tttcgccggt ttcacggtca taaatacggg
    17281 tgctctgacc aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt
    17341 caaccacttc agagcgtgcg ccgatgaagc agttatcttc aataatggtc gggttagcct
    17401 gcagcggctc cagcacgccg ccgataccca cgccaccgga aaggtggacg tttttaccaa
    17461 tctgcgcaca ggaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg
    17521 cgccgatgtt gacgtaagac ggcatcagca cggtgttacg ggcaatgaac gcgccctgac
    17581 gcactgccgc tggtggcacg acgcggaagc cttctttctg gaaacgtgct tcatcgtagt
    17641 tagcgaattt catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat
    17701 tatcattaat acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact
    17761 gaccgtcaat tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat
    17821 ttaccgcttc gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa
    17881 aagcggtttc aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa
    17941 cacatcaccc tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct
    18001 gcaactcgtt attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc
    18061 gctcgccaat ggttgtaatt cgggcgccat gaagcgaaat tcccagatcg gcaaaaattt
    18121 tcccgactcg cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc
    18181 ggtcggtatg ggtcggcaaa aacgtcactt cggtttcaac agtaaaatgg cgcaatttag
    18241 cgggttggcg acggggctgc ggtggctgcc agctactttg tgtcagcacc tgctccagac
    18301 caaaccgaat aacctcatga cgatctgcgg atagcgggct accatcgggt tccagcacta
    18361 taaaggtatc catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta
    18421 aattgcggcg gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc
    18481 agataaaaat ctcggtgcct ccacgcgtag cctgcgggct aagcagtacc agcggtttgc
    18541 ttaaatcatg ctgtaataaa tggcgtgcgt gccaggccag ttgatttggg ctatggcgga
    18601 caaaatagtt agcgcgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat
    18661 ccatgcgcag tagtgccagc gcctggagtt ggtgatggcg aacccgttcg cgcatatccg
    18721 gcgtgttttg cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca
    18781 gactttgctt ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca
    18841 ggcataccag atagcgcaga cgattttccg tttggacttc ttcggcaaac tgcttgatga
    18901 cttccgggtc ctgaatatcg cggcgctgag ctgtcaccga catcagcaag tgctggcgaa
    18961 ccagccaggc gaccagctgt gtttcgcgtg agttcagccc gtggagttcg gcaaaatgca
    19021 ctacatcctg agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa
    19081 acagcgcggc gatgaaaatc agctcagttg acggcagacg cggccagacg tccacacaca
    19141 acggatggcg ctggcgcgtt tcttcactgg caaaactctc cagtttcaac atcacgcgga
    19201 tagtatgttc atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat
    19261 gcgaccattg cggcatatac gcgccgagta cgctatggcg atgcattggc aatagcccgc
    19321 gccgcaccgc tccggggtga cgcagaatgc tcaaaaacag ttttcgcgct tccggaatat
    19381 tacacagagg ttgttgcaga tggcgacggg cgtggcgtaa ctggcgtagc gtggtggagt
    19441 aaatgccggt gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg
    19501 gctggcgcat aaatagtgtt tcatcacgca ggtcgatcag cgttccgcgt agctgaaact
    19561 catcgtcgat tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca
    19621 gttgcagcag catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca
    19681 tcatccgctc gaccggttcg ttaccttcac cactgtaatt caaacgctgg gcgacgctaa
    19741 gctggcgatc gaataacagg cgattatcgt aacgactgac gaccagatgc agggcaaagc
    19801 gaatgcgcca caatatatgc aggcattcgt ttaattccgc tcgctccgca gaggttaaga
    19861 agccaaaccc taccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt
    19921 gcagagtgtg gatatcgcgc aagccgccgg ggctactttt gatgtctggt tcaaggttgt
    19981 agctggtgcc gtggtaacgc tgatggcgct ggttctgttc ttcaactttc gccgcgtaga
    20041 acttgtcgga aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaacagcg
    20101 caacatcgcc aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata
    20161 acccttccag catgcactct tcaagcgtgc gcacgctatg acccacttcc agctttacat
    20221 cccagagcag cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac
    20281 ggcttaaaat cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac
    20341 cgacggcgac caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt
    20401 gaagaagctg gtcgataaac tcggtacgcg cctcaatcaa ctgttctgcg gagataccat
    20461 tgtcaaaggc atcacccagc caacgctgga aagtatcgat atgggctttt atcccaccga
    20521 cggttaattc atcacggggc caggcgcatg gattttgcgg ttgaccgtgc agggtgggga
    20581 gagcggtgtt tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa
    20641 ttattgcatt aaaaaagccg gcattcgccg gcttcatttt attcgtcgtg cgagattatc
    20701 gccgggatgg tgtcatcctt gcgtagcgtc agaatttcgc agccgttatc agtcaccaca
    20761 atagtatgct catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct
    20821 ttcatggtgc ggatctcttt tttacccgcg ttgaccattg gctcgatggt gaatgtcatc
    20881 cccggtttca gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttcg
    20941 tggaagccgc gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct
    21001 tcgacaaatt tctgaattgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg
    21061 cgtagcgcca ggtacaggct ttcttgcgtg atgcggcaca gacgttcgcc catgatggtc
    21121 ggcttaccga cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg
    21181 acatcaatgt taacgatatc gccatctttc agcagcttgg cgtcgtccgg gataccgtgg
    21241 cacaccactt cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag
    21301 gcagaaaccg cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg
    21361 gtgctgacgc ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg
    21421 ccagcgacgc gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct
    21481 gtccatcagc gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc
    21541 cgggcaaatt gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat
    21601 agaaaattca acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat
    21661 ttgtggtata aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc
    21721 gacaatctca ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg
    21781 gggtcggtaa tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc
    21841 atggcaactg tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc
    21901 cgttactgga acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc
    21961 atcaaccttg agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt
    22021 gcttctcgca aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg
    22081 aaagacgctg ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg
    22141 ctgactaact ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag
    22201 tctcaggacg gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag
    22261 ctggagaaac tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct
    22321 ctgtttgtaa tcgatgctga ccacgaacac attgctatca aagaagcaaa caacctgggt
    22381 attccagtat ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc
    22441 ccgggtaacg acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc
    22501 gtacgtgaag gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct
    22561 gagtaataag gcacgctcgt acgagccctt atagaccagg tagtacacgt ttggttaggg
    22621 ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc
    22681 acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg
    22741 agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca
    22801 tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag
    22861 gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc
    22921 tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag
    22981 acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac
    23041 agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc
    23101 gcgttgctgc gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggtg
    23161 ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg
    23221 ctgcaagcaa gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag
    23281 aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa attgcagaga
    23341 aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt
    23401 tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga
    23461 ctggctttat ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag
    23521 cagaagttgc tgcgatgtcc aagcagtctt aattatcgaa aaggagccgc ctgagggcgg
    23581 cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg
    23641 acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat
    23701 ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct
    23761 gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgcatgg ctcaggaaat
    23821 caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt
    23881 ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat
    23941 gctggcgacc gtaatgaacg gcctggcaat gcgtgatgca ctgcaccgcg cctatgtgaa
    24001 cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga
    24061 agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta caggtaaccc
    24121 gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt
    24181 gctgaaagca accaaagttg acggcgtgtt tactgctgat ccggcgaaag atccaaccgc
    24241 aaccatgtac gagcaactga cttacagcga agtgctggaa aaagagctga aagtcatgga
    24301 cctggcggcc ttcacgcttg ctcgtgacca taaattaccg attcgtgttt tcaacatgaa
    24361 caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga
    24421 ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggatctta
    24481 cccgtaatat gtttaatcag ggctatactt agcacacttc cactgtgtgt gactgtctgg
    24541 tctgactgag acaagttttc aaggattcgt aacgtgatta gcgatatcag aaaagatgct
    24601 gaagtacgca tggataaatg cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg
    24661 ggtcgtgctt ctcccagcct gctggatggc attgtcgtgg aatattacgg cacgccgacg
    24721 ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtactctgaa aatcaacgtg
    24781 tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcgtccga tcttggcctg
    24841 aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac ggaagaacgt
    24901 cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt
    24961 aacgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa agagatcagc
    25021 gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc tgcaatcaag
    25081 aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttctg atttcttgaa
    25141 cgacaaaaac gccgctcagt agatccttgc ggatcggctg gcggcgtttt gctttttatt
    25201 ctgtctcaac tctggatgtt tcatgaagca actcaccatt ctgggctcga ccggctcgat
    25261 tggttgcagc acgctggacg tggtgcgcca taatcccgaa catttccgcg tagttgcgct
    25321 ggtggccggc aaaaatgtca ctcgcatggt agaacagtgc ctggaattct ctccccgcta
    25381 tgccgtaatg gacgatgaag cgagtgcgaa acttcttaaa acgatgctac agcaacaggg
    25441 gagccgcacc gaagtcttaa gtgggcaaca agccgcttgc gatatggcag cgcttaagga
    25501 tgttgatcag gtgatggcag ccattgttgg cgctgctgga ctgttaccta cgcttgctgc
    25561 gatccgcgcg ggtaaaacca ttttgctggc caataaagaa tcactggtta cctgcggacg
    25621 tctgtttatg gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg atagcgaaca
    25681 taacgccatt tttcagagtt taccgcaacc tatccagcat aatctgggat acgctgacct
    25741 tgagcaaaat ggcgtggtgt ccattttact taccgggtct ggtggccctt tccgtgagac
    25801 gccattgcgc gatttggcaa caatgacgcc cgatcaagcc tgccgtcatc cgaactggtc
    25861 gatgggacgt aaaatttccg tcgattcggc taccatgatg aacaaaggtc tggaatacat
    25921 tgaagcgcgt tggctgttta acgccagcgc cagccagatg gaagtgctga ttcacccgca
    25981 gtcagtgatt cactcaatgg tgcgctacca ggacggcagt gttctggcgc agctgggtga
    26041 accggatatg cgtacgccaa ttgctcacac catggcatgg ccgaatcgcg tgaactctgg
    26101 cgtgaagccg cttgattttt gcaaactaag tgcgttgaca tttgccgcac cggattatga
    26161 tcgttatcca tgtctgaaac tggcgatgga ggcgtttgaa caaggccagg cagcgacgac
    26221 agcattgaat gccgcaaatg aaatcaccgt ggctgctttt cttgcgcaac aaatccgctt
    26281 tacggatatc gctgcgttga atttatccgt actggaaaaa atggatatgc gcgaaccaca
    26341 atgtgtggat gatgtgttat ctgttgatgc gaacgcgcgt gaagtcgcca gaaaagaggt
    26401 gatgcgtctc gcaagctgag gataatccgg ctacagagag tcgcgctatt tgttagcgta
    26461 gggcttcagt gatatagtct gcgccatctg atcgtaagta gttggcttta taaggtcagg
    26521 tatgccgtgg ttttacacgg cttttttttg tataggcttc agtattcctg agtaccgtaa
    26581 accctgtcag ggaataagaa acgcgtgatg ttgtctgcta ctcaaccact tagcgaaaaa
    26641 ttgccagcgc atggctgtcg tcatgttgcg atcattatgg acggcaatgg tcgctgggca
    26701 aaaaagcaag ggaagattcg tgcctttggg cataaagccg gggcaaaatc cgtccgccgg
    26761 gctgtctctt ttgcggccaa caacggtatt gaggcgttaa cgctgtatgc ctttagtagt
    26821 gaaaactgga accgaccagc gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg
    26881 ctagatagcg aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat tattggcgat
    26941 accagtcgct ttaactcgcg tttgcaagaa cgtattcgta aatctgaagc gctaacagcc
    27001 gggaataccg gtctgacgct gaatattgcg gcgaactacg gtggacgttg ggatatagtc
    27061 cagggagtca ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc agatcagata
    27121 gatgaagaga tgctaaacca gcatgtctgt atgcatgaac tggcccctgt agatttagta
    27181 attaggactg ggggggagca tcgcattagt aactttttgc tttggcaaat tgcctatgcc
    27241 gaactttact ttacagatgt tctctggccc gatttcgatg aacaagactt tgaaggggcg
    27301 ttaaatgcct ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca
    27361 gcctgatggg ggtcgctttt gctgaagtat cgcctgatat ctgcttttgt gttaataccc
    27421 gtcgtcatcg cggcgttgtt tctgttgccg ccggtggggt tcgccattgt aacgctggtg
    27481 gtctgtatgc tggcagcgtg ggaatgggga cagcttagcg gttttaccac tcgttcgcag
    27541 cgagtatggt tggcggtgtt atgcgggtta ttgttggcgc tgatgctttt tctgttgccg
    27601 gaatatcacc gaaatattca tcaaccgctg gttgaaatct cactttgggc ttcgctgggt
    27661 tggtggattg tcgcgctatt gctggtgctg ttttacccag gttccgcagc aatctggcgt
    27721 aactctaaaa cattgcgcat tatttttggc gtgctaacca ttgttccctt cttctggggc
    27781 atgctggcgt tacgggcctg gcactacgac gagaatcatt acagtggcgc aatatggctg
    27841 ctctatgtca tgatcctggt atggggcgct gactctggcg catatatgtt tggcaaattg
    27901 tttggtaaac ataaactggc accgaaggtt tctccgggta aaacctggca aggctttatc
    27961 ggtggactcg tgaccgcagc ggtaatctca tggggttatg gcatgtgggt gaatctcgac
    28021 gtcgctcccg tcaccttact catttgctct attgtcgcag cgttagcctc agtgctcggc
    28081 gatctgaccg aaagtatgtt taagcgtgaa gcgggaatta aggacagcgg tcatttaatt
    28141 ccaggacacg gtggtatttt agatcgtatt gatagcctga cggctgcggt accggtcttt
    28201 gcttgcttgt tgttactggt attcaggacg ctttaacgga aggtaatatg ctgagttttc
    28261 tctgggattt ggcttcgttc atcgttgcac tgggtgtact tatcaccgtg catgaatttg
    28321 gtcatttctg ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca atagggtttg
    28381 gtaaggcgct ctggcggcga actgataagc tcggcaccga atatgttatc gccctgatcc
    28441 cgttgggcgg ttatgtcaaa atgctggatg agcgcgcaga accggtcgtt ccggaactcc
    28501 gccaccatgc cttcaataat aaatctgtcg gccaacgagc ggcgattatt gccgcaggtc
    28561 cagttgcaaa cttcattttt gctatctttg cctactggct ggtttttatt attggtgtgc
    28621 ctggcgtacg tccggtggtt ggtgaaatag cagccaattc gatagctgcg gaagcacaaa
    28681 ttgcaccagg tacggaacta aaagccgtag atggtatcga aacgcctgat tgggatgccg
    28741 tgcgtttgca gttggtcgat aaaattggcg atgaaagcac caccattacg gtagcgccat
    28801 ttggcagcga ccaacggcgg gatgtaaagc tcgatttacg tcactgggcg tttgagcctg
    28861 ataaagaaga tccggtatct tcgttgggga ttcgtcctcg tgggccgcaa attgaacctg
    28921 tactggaaaa tgtgcagcca aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca
    28981 ggatcgttaa agtcgatggt cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc
    29041 gggataaccc gggtaaatcc ttagcgttag aaatcgaaag acaggggagt cccttgtctt
    29101 tgacattaat cccggagagt aaaccgggta atggtaaagc gattggtttt gtcggtattg
    29161 agccgaaagt cattcctttg cctgatgagt ataaagttgt acgccagtat gggccgttca
    29221 acgctatcgt cgaagccacg gacaaaacgt ggcagttgat gaagctgacg gtcagtatgc
    29281 tgggaaaatt gatcaccggt gatgtgaaac tgaacaacct cagtgggccg atctctatcg
    29341 ccaagggggc tgggatgaca gcggaactcg gggtagttta ttacctgccg tttcttgcgc
    29401 ttattagcgt gaacttaggg ataattaacc tgtttccgtt gcccgtactt gacggggggc
    29461 atctgctgtt ccttgcgatc gaaaagatca agggcggacc ggtatccgag cgggttcaag
    29521 acttttgtta tcgcattggc tcgattctgc tggtgctgtt aatggggctt gcacttttca
    29581 atgatttctc tcggttatga gagttagtta ggaagaacgc ataataacga tggcgatgaa
    29641 aaagttgctc atagcgtcgc tgctgtttag cagcgccacc gtatacggtg ctgaagggtt
    29701 cgtagtgaaa gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct
    29761 cagtatgccg gtgcgcacag gcgacacggt taatgatgaa gatatcagta ataccattcg
    29821 cgctctgttt gctaccggca actttgagga tgttcgcgtc cttcgtgatg gtgataccct
    29881 tctggttcag gtaaaagaac gtccgaccat tgccagcatt actttctccg gtaacaaatc
    29941 ggtgaaagat gacatgctga agcaaaacct cgaggcttct ggtgtgcgtg tgggcgaatc
    30001 cctcgatcgc accaccattg ccgatatcga gaaaggtctg gaagacttct actacagcgt
    30061 cggtaaatat agcgccagcg ttaaagctgt cgtgaccccg ctgccgcgca accgtgttga
    30121 cctaaaactg gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta acattgttgg
    30181 taaccatgct ttcaccaccg atgaactgat ctctcatttc caactgcgtg acgaagtgcc
    30241 gtggtggaac gtggtaggcg atcgtaaata ccagaaacag aaactggcgg gcgaccttga
    30301 aaccctgcgc agttactatc tggatcgcgg ttatgcccgt ttcaacatcg attctaccca
    30361 ggtcagtctg acgccagata aaaaaggtat ttacgtcacg gtgaacatca ccgaaggcga
    30421 tcagtacaag ctttctggcg ttgaagtgag cggcaatctt gccgggcact ccgctgaaat
    30481 tgagcaactg actaagatcg agccgggcga gctgtataac ggcaccaaag tgaccaaaat
    30541 ggaagatgac attaaaaagc ttcttggtcg ctatggttat gcctatccgc gcgtacagtc
    30601 gatgcctgaa attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg ttgatgcggg
    30661 taaccgtttc tatgtgcgta agatccgttt tgaaggtaac gatacctcga aagatgctgt
    30721 cctgcgtcgc gaaatgcgtc agatggaagg tgcatggctg gggagcgatc tggtcgatca
    30781 gggtaaggag cgtctgaacc gtctgggctt ctttgaaact gtcgataccg atacccaacg
    30841 tgttccgggg agcccggatc aggttgatgt agtttacaag gtgaaagaac gtaacaccgg
    30901 tagcttcaac ttcgggattg gttacggtac tgaaagtggc gtgagcttcc aggctggtgt
    30961 gcaacaggat aactggttag gtacgggtta tgctgttggt atcaacggga ccaaaaacga
    31021 ttaccagacc tatgctgaac tgtcggtaac caacccgtac ttcaccgtag atggcgtaag
    31081 cctcggtggt cgtctcttct ataatgactt ccaggcagat gacgccgacc tgtccgacta
    31141 taccaacaag agttatggta cagacgtgac gttgggcttc ccgatcaacg aatataactc
    31201 gctgcgtgca ggtctgggtt atgtacataa ctccctgtcc aacatgcagc ctcaggttgc
    31261 gatgtggcgt tatctgtact ctatgggtga acatccgagc acctctgatc aggataacag
    31321 cttcaaaacg gacgacttca cgttcaacta tggttggacc tataacaagc ttgaccgtgg
    31381 ttacttcccg acagatggtt cacgtgtcaa cctgaccggt aaagtgacca ttccgggatc
    31441 ggataacgaa tactacaaag tgacgttaga cacggcgact tatgtgccga ttgatgacga
    31501 tcacaaatgg gttgttctgg gtcgtacccg ttggggttat ggtgatggtt taggcggcaa
    31561 agagatgccg ttctatgaga acttctatgc cggtggttcc agcaccgtgc gtggcttcca
    31621 gtccaatacc attggtccga aagcagttta cttcccgcat caggccagta attatgatcc
    31681 ggactatgat tacgaatgtg cgactcagga cggcgcgaaa gatctgtgta aatcggatga
    31741 tgctgtaggc ggtaacgcca tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt
    31801 tattagcgat aagtatgcta actcggttcg tacttccttc ttctgggata tgggtaccgt
    31861 ttgggataca aactgggatt ccagccaata ttctggatat ccggactata gtgatccaag
    31921 caatatccgt atgtctgcgg gtatcgcatt acaatggatg tccccattgg ggccgttggt
    31981 gttctcctac gcccagccgt tcaaaaagta cgatggagac aaggcagaac agttccagtt
    32041 taacatcggt aaaacctggt aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat
    32101 gactttaggc gatcaatata agatcgccgg gccacgcaaa gaactgcacc ctccggtgca
    32161 aatgggatgg taaggagttt attgtgaaaa agtggttatt agctgcaggt ctcggtttag
    32221 cactggcaac ttctgctcag gcggctgaca aaattgcaat cgtcaatatg ggcagcctgt
    32281 tccagcaggt agcgcagaaa accggtgttt ctaacacgct ggaaaacgag ttcaaaggcc
    32341 gtgccagcga actgcagcgt atggaaaccg atctgcaggc taaaatgaaa aagctgcagt
    32401 ccatgaaagc gggcagcgat cgcactaagc tggaaaaaga cgtgatggct cagcgccaga
    32461 cttttgctca gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac
    32521 gcggcaaact ggttactcgt atccagactg ctgtgaaatc cgttgccaac agccaggata
    32581 tcgatctggt tgttgatgca aacgccgttg cttacaacag cagcgatgta aaagacatca
    32641 ctgccgacgt actgaaacag gttaaataag taatgccttc aattcgactg gctgatttag
    32701 cgcagcagtt ggatgcagaa ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt
    32761 ccatgcaatc tgcacaaaca ggtcacatta cgttcatggt taacccaaaa taccgtgagc
    32821 atttaggctt gtgccaggcg tccgcggttg tcatgaccca ggacgatctt cctttcgcga
    32881 aaagtgccgc gctggtagtg aagaatccct acctgactta cgcgcgcatg gcgcaaattt
    32941 tagataccac gccgcagccc gcgcagaaca ttgcacccag tgcggtgatt gacgcgacgg
    33001 cgaagctggg taacaacgta tcgattggcg ctaacgcggt gattgagtcc ggcgttgaac
    33061 tgggcgataa cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg
    33121 caggttcgcg tctctgggcg aacgtaacca tttaccatga gatccagatc ggtcagaatt
    33181 gcctgatcca gtccggaaca gtggtaggcg cagacggctt tggttatgcc aacgatcgtg
    33241 gtaactgggt gaagatccca cagattggtc gggtaattat tggcgatcgc gtggagatcg
    33301 gtgcctgcac tactattgat cgtggcgcgc tggatgacac tgttattggc aatggtgtga
    33361 tcattgataa ccagtgccag attgcacata acgtcgtgat tggcgacaat acggcagttg
    33421 ccggtggcgt cattatggcg ggcagcctga aaattggtcg ttactgcatg atcggcggag
    33481 ccagcgtaat caacgggcat atggaaatat gcgacaaagt gacggttacg ggcatgggta
    33541 tggtgatgcg tcccatcact gaaccaggcg tctattcctc aggcattccg ctgcaaccca
    33601 acaaagtctg gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg agcaagcgtc
    33661 tgaaatcgct tgagcgcaag gttaatcaac aagactaacg ttccatcttt tgttcgccaa
    33721 actttacggc ctgtctcatt cttacgattg cggcaggccg tgttattatt gtcgtttctt
    33781 atattttgac aggaagagta tcttgactac taacactcat actctgcaga ttgaagagat
    33841 tttagaactt ctgccgcacc gtttcccgtt cttactggtg gatcgcgtgc tggattttga
    33901 agaaggtcgt tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg
    33961 ccatttccct ggaaaaccga ttttcccggg tgtgctgatt ctggaagcaa tggcacaggc
    34021 aacaggtatt ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc tgtactactt
    34081 cgccggtatt gacgaagcgc gctttaagcg cccggtcgtg cctggcgatc aaatgatcat
    34141 ggaagtcact ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt
    34201 cgacggtaaa gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg aggcctgata
    34261 cgtgattgat aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcgat
    34321 tggcgcgaac gctcacattg gtcctttttg tatcgttgga ccccatgtcg aaattggtga
    34381 gggtaccgta ctgaaatctc acgttgtcgt gaatggtcat actaaaattg gccgcgataa
    34441 tgagatttat cagttcgcct ccatcggcga agttaaccag gatctgaaat atgctggcga
    34501 accgacccgc gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg
    34561 tggcacagtc cagggcggtg gattgacgaa ggtgggcagc gacaacttac tgatgatcaa
    34621 cgcgcacatt gcgcacgatt gtacggtagg taaccgctgc atcctcgcca acaacgcaac
    34681 gctggcgggt cacgtatcgg ttgacgactt cgcgatcatc ggcggcatga ccgcagtcca
    34741 tcagttctgc atcattggtg cgcatgtgat ggttggcggc tgctccggtg tggcgcagga
    34801 cgtccctcct tatgtcattg cgcagggtaa ccacgcaacg ccgttcggtg tcaatatcga
    34861 agggctgaag cgccgcggat tcagccgtga ggcgattacc gctatccgca atgcgtataa
    34921 gctgatttat cgtagcggta agacgctcga tgaagtgaaa ccggaaattg ctgaactggc
    34981 ggaaacatat ccggaagtga aagcctttac cgatttcttt gcacgctcaa cgcgcggtct
    35041 gattcgttaa tgactgaaca gcgtccatta acgattgccc tggtcgccgg agaaacctcc
    35101 ggcgatatcc tgggggccgg tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc
    35161 tttgttggtg ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg
    35221 gaagaactgg cagtgatggg cattgttgaa gtgctcggtc gtctgcgtcg cttactacat
    35281 attcgtgccg atctgacaaa gcgttttggc gaactgaagc cagatgtttt tgttggtatt
    35341 gatgcgcctg acttcaatat tactcttgaa ggtaacctca aaaagcaggg tatcaaaacc
    35401 attcattacg tcagtccgtc cgtctgggcg tggcgacaga aacgcgtttt caaaataggc
    35461 agagccaccg atctggtgct cgcatttctg cctttcgaaa aagcgtttta tgacaaatac
    35521 aacgtaccgt gccgctttat cggtcatacc atggctgatg ccatgccatt agatccagat
    35581 aaaaatggtg cccgtgatgt gctggggatc ccttacgatg cccactgtct ggcattgttg
    35641 ccgggcagcc gtggtgcaga agtcgaaatg cttagtgccg atttcctgaa aacggcccag
    35701 cttttgcgcc agacatatcc ggatctcgaa atcgtggtgc cgctggtgaa tgccaaacgc
    35761 cgtgagcagt ttgaacgcat caaagctgca gtcgcgccag acctgtcggt tcatttgctg
    35821 gatggaatgg gccgtgaggc gatggtcgcc agtgatgcgg cgctactggc gtcgggtacg
    35881 gcagcactgg agtgtatgct ggctaaatgc ccgatggtag tgggatatcg catgaagcct
    35941 ttcaccttct ggctggcgaa gcgactggtg aaaactgatt atgtctcgct gccaaatctg
    36001 ctggcgggca gagagttagt caaagagtta ttgcaggaag agtgtgagcc gcaaaaattg
    36061 gctgcggcgc tgttaccgct gttggcgaac gggaaaacca gccatgcgat gcacgacacc
    36121 ttccgcgagc tgcatcagca gatccgctgc aatgccgatg agcaggcggc acaagccgtt
    36181 ctggagttag cacaatgatc gaatttgtct atccgcacac gcagctggtt gcgggtgtgg
    36241 atgaagtcgg acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg atccttgacc
    36301 cggcgcgccc gattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa cgccgtctgg
    36361 cgctctgtga agagatcaaa gagaaagcgt tgagctggag tctgggccgc gcggaacctc
    36421 acgaaatcga cgaactgaat attttacatg cgaccatgct ggcgatgcag cgtgccgtcg
    36481 ctggactgca tattgcgccg gaatatgtgt tgattgatgg taaccgctgc ccgaaattac
    36541 cgatgccttc gatggctgtg gtgaaaggcg atagccgcgt accggaaatc agtgccgcgt
    36601 ctatcctggc gaaagtgacg cgtgacgccg aaatggcggc gctggatatt gttttcccgc
    36661 aatatggttt tgcccaacac aaagggtatc caaccgcttt tcatctggaa aaactggctg
    36721 aacacggcgc gaccgaacac catcggcgca gctttgggcc tgtcaaacgc gcactgggac
    36781 ttgcgtcctg attcttgtgt cgagattaag taaaccggaa tctgaagatg tctgaaccac
    36841 gtttcgtaca cctgcgggtg cacagtgact actcgatgat cgatggcctg gccaaaaccg
    36901 caccgctggt aaaaaaggcg gcagcgttgg gtatgccagc actggcgatc accgatttca
    36961 ccaacctttg cggtctggtg aagttctacg gagcgggaca tggcgcaggg attaagccca
    37021 tcgtcggggc ggattttaac gtccagtgcg acctgctggg tgatgagtta actcacctga
    37081 cggtgctggc ggcgaacaat accggctatc agaatctgac gttgctgatc tcaaaagcgt
    37141 atcagcgcgg gtacggtgcc gccgggccga tcatcgatcg cgactggctt atcgaattaa
    37201 acgaagggtt gatccttctt tccggcgggc gcatgggcga cgttggacgc agtcttttgc
    37261 gtggtaacag cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac ttcccggatc
    37321 gctattttct cgagctgatc cgcaccggca ggccggacga agaaagctat ctgcacgctg
    37381 cggtggaact ggcggaatca cgcggtttgc ccgttgtggc gaccaacgac gtgcgcttta
    37441 tcgacagcag cgactttgac gcacacgaaa tccgcgtcgc gatccacgac ggctttaccc
    37501 tcgacgatcc taaacgcccg cgtaactatt cgccgcagca atatatgcgt agcgaagagg
    37561 agatgtgcga gctttttgcc gacattcccg aagcccttgc caacaccgtt gagatagcca
    37621 aacgttgtaa cgtaactgtg cgtctgggtg aatacttctt gccgcaattc ccgaccgggg
    37681 acatgagtac cgaagattat ctggtcaagc gtgcaaaaga aggcctggaa gagcgtctgg
    37741 catttttatt ccctgacgag gaagaacgtc ttaagcgtcg tccggagtat gacgaacgtc
    37801 tggatactga acttcaggtt atcaaccaga tgggcttccc gggttacttc ctcatcgtta
    37861 tggaatttat ccagtggtcg aaagataacg gtgtaccggt agggccaggc cgtggttctg
    37921 gtgcagggtc actggtggcc tacgcgttaa aaatcaccga tctcgatccg ctggaatttg
    37981 acctgctgtt cgaacgtttc cttaacccgg aacgtgtctc catgcctgac ttcgacgttg
    38041 acttctgtat ggagaaacgt gaccaggtta tcgaacatgt ggcggacatg tacggtcgcg
    38101 atgcggtatc gcagattatc accttcggta cgatggcggc gaaagcggtg atccgcgacg
    38161 taggccgtgt attggggcat ccgtacggat ttgtcgatcg tatctcgaaa ctgatcccgc
    38221 ccgatccggg tatgacgctg gcgaaagcgt ttgaagccga gccgcagctg ccggaaatct
    38281 acgaagcgga tgaagaagtt aaggcgctga tcgacatggc gcgcaaactg gaaggggtca
    38341 cccgtaacgc cggtaagcac gccggtgggg tggttatcgc gccgaccaaa attactgatt
    38401 ttgcgccgct ttattgcgat gaagagggca aacatccggt cacccagttt gataaaagcg
    38461 acgttgaata cgccgggctg gtgaagttcg acttcctcgg tttgcgtacg ctcaccatca
    38521 tcaactgggc gctggagatg atcaacaagc ggcgggcgaa gaatggcgag cccccgctgg
    38581 atatcgccgc gatcccgctg gatgacaaga aaagcttcga catgctgcaa cgctcggaaa
    38641 ccacggcggt attccagctt gaatcgcgcg gcatgaaaga cctgatcaag cgtctgcaac
    38701 ctgactgctt cgaagatatg atcgcactgg tggcactgtt ccgccccggc ccgttgcaat
    38761 cagggatggt ggataacttt atcgaccgta aacatggtcg cgaagagatc tcctatccgg
    38821 acgtgcagtg gcagcatgaa agcctgaaac cggtactgga gccaacctac ggcatcatcc
    38881 tgtatcagga acaggtcatg cagattgccc aggtgctttc tggttatacc ctcggtggcg
    38941 cggatatgct gcgtcgtgcg atgggtaaga aaaagccgga agagatggct aagcagcgtt
    39001 ctgtatttgc tgaaggtgca gaaaagaacg gaatcaacgc cgaactggcg atgaaaatct
    39061 tcgacctggt ggagaaattc gcgggttacg gatttaacaa atcgcactcg gcggcctatg
    39121 ctttggtgtc atatcaaacg ttatggctga aggcacatta tccggcagag tttatggcgg
    39181 cggtaatgac cgccgatatg gacaacaccg agaaggtggt gggcctggtg gatgagtgct
    39241 ggcggatggg gctgaaaatc ctgccaccag atataaactc cggtctttac catttccacg
    39301 tcaacgacga cggcgaaatc gtgtatggta ttggcgcgat caaaggggta ggtgaaggcc
    39361 cgattgaggc catcatcgaa gcccgtaata aaggcggcta cttccgcgaa ctgtttgatc
    39421 tctgcgcccg taccgacacc aaaaagttaa accggcgggt gctggaaaaa ctgatcatgt
    39481 ccggggcgtt tgaccgtctt gggccacacc gcgcggcgct gatgaactcg ctgggcgatg
    39541 cgttaaaagc ggcagatcaa cacgcgaaag cggaagctat cggtcaggcc gatatgttcg
    39601 gcgtgctggc cgaagagccg gaacaaattg aacaatccta cgccagctgc caaccgtggc
    39661 cggagcaggt ggtgttagat ggggaacgtg aaacgttagg tctgtacctc acgggacacc
    39721 ctatcaacca gtatttaaaa gagattgagc gttatgtcgg aggcgtaagg ctgaaagaca
    39781 tgcacccgac agaacgtggt aaagtcatca cggctgcggg gctcgttgtt gccgcgcggg
    39841 ttatggtcac caagcgcggc aatcgtatcg gtatctgcac gctggatgac cgttccgggc
    39901 ggctggaagt gatgttgttt actgacgccc tggataaata ccagcaattg ctggaaaaag
    39961 accgcatact tatcgtcagc ggacaggtca gctttgatga cttcagcggt gggcttaaaa
    40021 tgactgctcg cgaagtgatg gatattgacg aagcccggga aaaatatgct cgcgggcttg
    40081 ctatctcgct gacggacagg caaattgatg accagctttt aaaccgactc cgtcagtctc
    40141 tggaacccca ccgctctggg acaattccag tacatctcta ctatcagagg gcggatgcac
    40201 gcgcgcgatt gcgttttggc gcgacgtggc gtgtctctcc gagcgatcgt ttattaaacg
    40261 atctccgtgg cctcattggt tcggagcagg tggaactgga gtttgactaa tacaggaata
    40321 ctatgagtct gaatttcctt gattttgaac agccgattgc agagctggaa gcgaaaatcg
    40381 attctctgac tgcggttagc cgtcaggatg agaaactgga tattaacatc gatgaagaag
    40441 tgcatcgtct gcgtgaaaaa agcgtagaac tgacgcgtaa aatcttcgcc gatctcggtg
    40501 catggcagat tgcgcaactg gcacgccatc cacagcgtcc ttataccctg gattacgttc
    40561 gcctggcatt tgatgaattt gacgaactgg ctggcgaccg cgcgtatgca gacgataaag
    40621 ctatcgtcgg tggtatcgcc cgtctcgatg gtcgtccggt gatgatcatt ggtcatcaaa
    40681 aaggtcgtga aaccaaagaa aaaattcgcc gtaactttgg tatgccagcg ccagaaggtt
    40741 accgcaaagc actgcgtctg atgcaaatgg ctgaacgttt taagatgcct atcatcacct
    40801 ttatcgacac cccgggggca tatccgggcg tgggcgcaga agaacgtggt cagtcggaag
    40861 ccattgcacg caacctgcgt gaaatgtctc gcctcggcgt accggtagtt tgtacggtta
    40921 tcggtgaagg tggttctggt ggtgcgctgg cgattggcgt gggcgataaa gtgaatatgc
    40981 tgcaatacag cacctattcc gttatctcgc cggaaggttg tgcgtccatt ctgtggaaga
    41041 gcgctgacaa agcgccgctg gcggctgaag cgatgggtat catcgctccg cgtctgaaag
    41101 agctgaaact gatcgactcc atcatcccgg aaccgctggg cggtgctcac cgtaacccgg
    41161 aagcgatggc ggcgtctctg aaagcgcagc tgctggcgga tctggccgat ctcgacgtgt
    41221 taagcactga agatttaaaa aatcgtcgtt atcagcgcct gatgagctac ggctacgcgt
    41281 aatcggcaaa tgttctgaaa agggtcactt cggtggccct tttttatcgc cacggtttga
    41341 gcaggctatg attaaggaag gattttccag gaggaacaca tgaacatcat tgccattatg
    41401 ggaccgcatg gcgtctttta taaagatgag cccatcaaag aactggagtc ggcgctggtg
    41461 gcgcaaggct ttcagattat ctggccacaa aacagcgttg atttgctgaa gtttatcgaa
    41521 cataacccac gaatttgcgg cgtgattttt gactgggatg agtacagtct cgatttatgt
    41581 agcgatatca atcaacttaa tgaatatctc ccgctttatg ccttcatcaa cacccactcg
    41641 acgatggatg tcagcgtgca ggatatgcgg atggcgctct ggttttttga atatgcgctg
    41701 gggcaggcgg aagatatcgc cattcgtatg cgtcagtaca ccaacgaata tcttgataac
    41761 attacgccgc cgtttacgaa agccttgttt acctacgtca aagagcggaa gtacaccttt
    41821 tgtacgccgg ggcatatggg cggtaccgca tatcaaaaaa gcccggttgg ctgtctgttt
    41881 tatgattttt tcggcgggaa taccctcaag gcggacgtct ctatttcggt caccgagctt
    41941 ggttcgttgc tcgaccacac cgggccacac ctggaagcgg aagagtacat cgcgcggacg
    42001 tttggcgcgg aacagagtta tatcgttact aatggaacat cgacgtcgaa caaaattgtg
    42061 ggtatgtacg ccgcgccatc cggcagtacg ctgttgatcg accgcaattg tcataaatcg
    42121 ctggcgcatc tgttgatgat gaacgatgta gtgccagtct ggctgaaacc gacgcgtaat
    42181 gcgttgggga ttctgggtgg gatcccgcgc ggtgaattta ctcgcgacag catcgaagag
    42241 aaagtcgctg ccaccacgca ggcacaatgg ccggttcatg cggtgatcac caactccacc
    42301 tatgatggct tgctctacaa caccgactgg atcaaacaga cgctggatgt cccgtcgatt
    42361 cacttcgatt ctgcctgggt gccgtacacc cattttcatc cgatctacca gggtaaaagt
    42421 ggtatgagcg gtgagcgtgt tgcgggaaaa gtgatcttcg aaacgcagtc gacccacaaa
    42481 atgctggcgg cgttgtccca ggcgtcgctg atccacatta aaggcgagta tgacgaagag
    42541 gcgtttaacg aagcctttat gatgcatacc accacctcgc ccagttatcc cattgttgct
    42601 tcggttgaga cggcggcggc gatgctgcgt ggtaatccgg gcaaacggct gattaaccgt
    42661 tcagtagaac gagcgctgca ttttcgcaaa gaggtccagc ggctgcggga agagtctgac
    42721 agctggtttt tcgatatctg gcaaccgccg caggtggatg aagccgaatg ctggcacgtt
    42781 gcgcctggcg aacagtggca cggctttagc gatgcggatg ccaatcacat gtttctcgat
    42841 ccggttaaag tcactatttt gacaccggga atggacgagc agggcaatat gagcgaggag
    42901 gggatcccgg cggcgctggt ggcaaaattc ctcgacgaac gtgggatcgt agtagagaaa
    42961 accggccctt ataacctgct gtttctcttt agtattggca tcgataaaac caaagcaatg
    43021 ggattattgc gtgggttgac ggaatttaag cgctcttacg atctcaacct gcggatcaaa
    43081 aatatgctgc ccgatctcta tgcagaagat cccgatttct accgcaatat gcgtattcag
    43141 gatctggcgc aagggatcca taagctgatt cgtaaacacg atcttcccgg tttgatgttg
    43201 cgggcatttg ataccttgcc ggagatgatc atgacgccac atcaggcatg gcagagacag
    43261 attaaaggcg aagtagaaac cattgcgctg gaacaactgg tcggtagagt atcggcaaat
    43321 atgatcctgc cttatccacc cggcgtaccg ctgctgatgc cgggagaaat gcttaccgaa
    43381 gagagccgca cggtactcga ttttctactg atgctttgtt ccgtagggca acattacccc
    43441 ggttttgaaa cggatattca cggcgcgaaa caggacgaag acggcgttta ccgcgtacga
    43501 gtcctaaaaa tggcgggata acttgccaga gcggcttccg ggcgagtaac gtgctgttaa
    43561 caaataaagg agacgttatg ctgggtttaa aacaggttca ccatattgcg attattgcga
    43621 cggattatgc gatgagcaaa gctttctact gcgatattct tggtttcacg ctgcaaagcg
    43681 aagtctatcg cgaagcgcgc gactcgtgga aaggggattt ggcgcttaat gggcaatatg
    43741 tgattgagct tttctcattt ccgttcccgc cggagcgccc cagccgaccg gaagcttgcg
    43801 gcctgcgtca tctggctttt agcgttgatg atatcgatgc ggcagtggcg caccttgaaa
    43861 gccataacgt gaagtgtgaa gccatccgtg tcgatccata cacgcaaaaa cgcttcacgt
    43921 tctttaacga tccggacggg ctgccgttgg aactgtatga gcagtaaggc ttgtcatcgc
    43981 cgcatttgcc cggtaacgtg ccgggcattg ctactgtaaa atcgcaccat catgacactc
    44041 acgctcaata gacaacttct ctcctcacgc cagattctgg tggcctttag cggcggcctt
    44101 gattctactg tcttactgca tcaactggtg cagtggcgga cggaaaatcc gggcgtcact
    44161 ctgcgcgcta tccatgtgca tcacggttta agcgccaatg ctgatgcctg ggtgaaacat
    44221 tgtgaaaaca tctgccaaca gtggcaggtg ccgctggtgg tcgaacgtgt gcagcttgcg
    44281 caagaggggc taggtattga ggcccaggcg cggcaggcac gttatcaggc atttgcccgt
    44341 accttgttgc ccggtgaagt gctggttaca gcgcaacatc tcgacgatca atgtgaaacc
    44401 tttctgctgg cgctaaaacg cggtagcggc cctgccgggc tttcggctat ggcggaagtt
    44461 tcggagtttg ccggaacacg gcttatccgc ccgttgcttg cccgcacgcg gggggaactg
    44521 gagcagtggg cgcgtcagta tgatttacgc tggattgaag acgaaagtaa tcaagacgat
    44581 tcctacgatc gtaacttctt gcgcctgcgc gtagtgccgt tattgcagca gcgttggccg
    44641 cattttgccg aagcgacggc ccgcagcgcc gcactttgcg ccgaacaaga gagcctgctg
    44701 gatgaactgc tggcggatga tttagcgcac tgccaaacgc aacagggaac gttaaagatt
    44761 gcgccaatgc tggcaatgag cgatgcccgt cgcgcggcga ttatccgccg ctggctggca
    44821 gggcagaatg cacctatgcc ttcccgcgag gcgctggtgc ggatctggca ggaagtggcg
    44881 ctggcgcggg aagatgcttc cccctgttta cgtttgggcg cgtttgaaat ccgccgctat
    44941 cagtcgcaac tgtggtggat taaatccgtt gccgggcaaa gcgaaaccat tgtgccgtgg
    45001 cagacgtggc tacaaccgct ggaattaccg gcggggctgg gaagtgtaca gcttactgcg
    45061 ggaggcgata ttcgccctcc gcgtgcagac gaagcggtca gcgtgcgttt caaagcgcca
    45121 ggactgctgc atattgtcgg gcgcaacggt ggacgtaagc tgaagaaaat ctggcaagag
    45181 ttaggcgttc cgccgtggct acgtgacacc acgccactgc tgttttatgg cgaaacgctg
    45241 attgcggcgg caggggtatt tgtgacgcaa gaaggtgtgg ctgtaggtga gaatggcgtc
    45301 agttttgtct ggcagaaaac gcttagtcaa gtgaaagccg gataagacgc atcaaacgtc
    45361 gcatccggcg aaagtcaatc aggactcgct caccaccacc gtaccgattt ccgggtggct
    45421 aaagctggta attttatcca gacgcagctc gcgggttgca ccagcggcct cgacgaccag
    45481 atactccaca tttttgcggg agactaaatc gctggccttc gcctgcaatt tttcgccatc
    45541 tttcagctca agtgtcagca ttaaatgatg ctggcaggcg agctcaagat tatcgtaatc
    45601 atcacaattg attggttgat acgtatcatt cattgacata atcgctcacc agtaagtttg
    45661 ccgcagcgta tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg gcagccattt
    45721 cattcagcac tttcaaaacg cagcccagcg cgtccggaac gtatcctaag tctccgctgg
    45781 cgatttccgc gtaccgcttg cgtattaact cacaatattt ttccacatgc cctcctgtca
    45841 gcactctgac ttaaccgtgg atgcaagtct aagcctacga agataaactc tgtttcgcaa
    45901 ggtgactata ccacactcat ttctgcaata tcagcgccgc aactgcacgt attccgttac
    45961 aatggcctcc tgattcgaaa ggagttttct tatggcgctt aaagcgacaa tttataaagc
    46021 gacggttaat gtggccgatc tcgaccgcaa ccagtttctc gatgcctctc tgacgttggc
    46081 gcgccatcct tcagaaaccc aggagcgtat gatgctgcgc ctgctggcgt ggctaaaata
    46141 tgccgatgaa cgtctgcaat ttacccgtgg tttgtgtgcc gatgatgagc cggaagcgtg
    46201 gctgcgtaac gatcatttgg gtattgattt gtggattgag ctggggctgc cggatgagcg
    46261 acggattaag aaagcctgca cccaggccgc agaagtggcg ctgtttgcct ataatagtcg
    46321 ggcggcgcaa atctggtggc agcaaaatca gagcaaatgt gcgcagtttg ccaatctttc
    46381 cgtctggtat ctggacgacg agcagctggc gaaagtgagc gcctttgccg atcgtaccat
    46441 gacgctgcag gcaacgattc aggatggtgt tatctggtta tcggatgata agaataatct
    46501 ggaagtgaac ttaaccgtct ggcaacaacc ttcatgattg tgatttcccg acatgttgct
    46561 atccctgatg gcgagtttga gatcaccgcc attcgtgcgc agggcgcggg cgggcagcat
    46621 gttaataaga cctcaacggc tattcatctg cgttttgaca ttcgggcgtc cagcctgcca
    46681 gagtattaca aagagcgtct gctcgccgcc agccatcatt tgatcagcag tgacggagtg
    46741 attgtcatta aggcacagga ataccgcagt caggaactga accgcgaagc ggcgctggcc
    46801 cggctggtgg cagtgattaa agatttaaca acagaacaaa aagcccgaag acctacgcgg
    46861 cccacccgtg catcgaaaga gcgcaggctg gcatcgaaag cacaaaaatc aagcgtgaag
    46921 gcgatgcgcg gcaaagtgcg cagcggtcgg gagtaaaaag aaggaatgga tggtgaaaaa
    46981 agcgatagtg acagcgatgg ctgtaatcag cctttttact ctgatgggat gtaataatcg
    47041 ggccgaagtc gatacgcttt ccccggcgca ggctgccgaa ctgaaaccga tgccgcaaag
    47101 ttggcgcggc gtgctgccgt gtgctgattg cgaaggaatc gaaacctctc tgttcctcga
    47161 aaaagatgga acctgggtga tgaatgaacg ttatctgggg gcacgtgaag agccttcctc
    47221 tttcgcttcc tacggtacat gggcgcgaac tgccgacaag ctggtattaa ccgacagcaa
    47281 aggtgaaaag tcatattatc gagcgaaagg ggatgcactg gagatgctcg atcgtgaagg
    47341 taatcccatt gaatcgcagt tcaactacac gctggaaccg gcaaaatcga gcatgccaat
    47401 gacgccgatg accctgcgtg gcatgtattt ctatatggct gatgcggcga cctttactga
    47461 ttgcgcgact ggaaaacgtt ttatggtggc gaataacgca gagctggagc gtggctacct
    47521 ggctgcgcgc ggcaacggcg aaaaaccggt gttactgtca gtagaaggtc actttacgct
    47581 tgaggctaat ccggataccg gtgcgccgac caaagtatta gcgcccgata cggcaggtaa
    47641 attttacccg aaccaggatt gcagtagttt ggggcagtaa cccgtcttga ggcagaaaca
    47701 aatgaaatat tgcctcatgc gctgcgctta tcaggcctaa agcatacaat gcaatatttt
    47761 gaattagcac gattttgtag gccggataag gcgttcacgc cgcatccggc attttgcgca
    47821 cgaggctggc ttttatcact gagtcagtcc aacaaagcga cttgctttaa tataaatccc
    47881 tggcttcagg tgcccaatat actgtaactg cgtttctggt ttaaatgcag agacatcacc
    47941 actacgcata tgcatcagat cggcagggct aatccagcct gattgcgcca gcgtaagcgg
    48001 atgcccggct ttggcaaatg catccgtgac aaattccgaa caaaaccacg actttttgtc
    48061 tccttcgcca acgttactta actgcgcttt cgccaggccg ctgacgcact gttggcgaaa
    48121 atcctcggag aacggattca gtgagcacat ctggcgagtc accataaatg gaataaattc
    48181 gacaatgccg cgatagttat aaccgctatc tttaatttta ttggcgaacg cggtgatttc
    48241 tgtggcttgt tgcggggtaa ggtccgggac tcgtaagacg aaaagcttat cactgtgctt
    48301 catggctttt ttaagggaaa caatctggac gccagcgcct gtcgcttcgg caacgttatt
    48361 atttcccaaa tagattgcaa cgtgactcac agaggaagtg ctgaatacgc ggataccaaa
    48421 tgaggttacc ccaaggcttg aggagaaaag caaatcgccg ggtttgagat ctggttccgt
    48481 tatttctttt attgattgtt cggtgaaaga gctttgatgc tggaatttaa cagcccatgt
    48541 tttcgcctcg gcatttaccg ctgtggctga tgagtctggc tggctgatat caacggtaca
    48601 ggccgaaagt aaaagaaagc aggggagaag caggcggcag tacgcctttg gtttatccat
    48661 tttatacaat ccatgtaaaa aagggccccg aaattcagga ccctttctgg catcagcctt
    48721 taatctgttt caccagataa tcgacgatgt caccagtctt aattaactgt ttctcgccgt
    48781 tacgacgata tttatattcg atatcatcgt tgtcgaggtt acggtcgccc agcacaatag
    48841 tgtgcggaat accgatcagt tccatatcag caaacatcac gcccggacgc tctttgcggt
    48901 catccagcag cacttcgata ccttgtgcgc gcagttcgct gtacagtttc tcagcaagtt
    48961 cctgtacgcg gaaggatttg tgcatgttca ttggcagaat cgccacctgg aacggcgcga
    49021 tagcgtcagg ccatacgatg ccgcgttcgt cgtagttctg ctcaatcgcc gctgctacca
    49081 cacgcgttac gccgataccg tagcaaccca tcgtcaggat ttggttacgg ccatcttcac
    49141 cctgtacgga ggctttcagt gcttcggagt acttggtacc cagctggaag atgtgaccga
    49201 cttcgatacc acgtttgatc agcagcgtac cctgaccatc cgggcttgga tcgccagcca
    49261 ccacgttgcg gatatcagca atttccgggg tagcgacatc gcgatcccag ttgataccga
    49321 agtagtgttt accatcgatg ttagcaccag cagcgaaatc actcatcgcc gcaacggtac
    49381 ggtcaatcac caccggaatc ggcatgttta ccggacccag tgaacccgga ccggctttaa
    49441 ccacggcacg aatttcttct tcggtcgcaa aagtcagcgg gctggcaacc tgcggcagtt
    49501 tttctgcttt aacttcgttc agctcgtggt caccgcgcac cagcagcgca accagcggga
    49561 agctactgcc ttcaacggct ttaaccagca gagtcttaac cgttttctca atcggcagat
    49621 tgaactgttc aaccagttcc gcgatggttt tcgcgttcgg cgtatcaacc agcgtcattt
    49681 cctgggtagc agcagcgcgc ggttctttcg gcgcgatagc ttctgccagt tcaatgttcg
    49741 ctgcgtagtc agaagtatcg gagaagacca catcgtcttc accgctctgc gccagcacct
    49801 gaaattcgtg agaggcgcta ccgccgatag aaccggtatc ggcctgtacg gcgcggaaat
    49861 ccagccccat gcggctgaag attttgctgt aggccgcata cattgcatcg taggtttcct
    49921 gcagggattc ctgagaagta tggaaagagt aagcatcttt catcaggaat tcgcgggaac
    49981 gcatgacgcc aaaacgcgga cgcacttcgt cgcggaactt ggtctggatc tgatagaagt
    50041 tcagcggcag ttgtttgtaa gagctcagct cgttacgaat caggtcagtg ataacttctt
    50101 catgagttgg gccgagtacg aacggacgct cgccacggtc aacaaaacgc agcagttccg
    50161 gaccgtactg ttcccaacga ccactctctt gccacaaatc ggctggctga accaccggca
    50221 ttaacacctc gatcgcaccg gcgttgttca tctcttcacg cacgatgttt tcgacttttt
    50281 tcagaacgcg cacgccggtc ggcagccagg tatataaccc ggaggccagc ttgcggatca
    50341 tcccggcgcg cagcatcagc tgatggctga tcacctcggc gtcggcaggt gtctccttga
    50401 gagtggagag caggtattgg ctagtacgca tgttgttacg gttccagttg gaaggtagaa
    50461 caggctcaag gcgagcctgg gacaaaaaaa gtgatttagt ttaccagtgc aaaagaaatg
    50521 tcaaaagaga agggcgtgaa tttaacgcgg ttccagcgca aagacttcaa aacctgcgtc
    50581 ggtgacgcgc cagcgaacgt taaaatcatg caaccagacg gcataggttt tgcccgtttc
    50641 ctcaccttta cgataggccg ggcgcgggtc ctgcgccagt acttcgcgga taaacagcgt
    50701 taactgcgga taacgcttct ccagcgtcaa aagctgcttt tcgacctctg cggtaaaaca
    50761 aaccgccatt tctgccgctg gcgcactttg cgcatagctg gcactggcat cgggaagcga
    50821 ttcagcaaag gggagatacg gtttgatatc cactaccggc gtaccatcga ccagatccag
    50881 actgccgagc ttcagaatca cgctgtcttt atggcaaaca acctctttca gctctaccag
    50941 cgacatacca atcgggttgg ggcgaaaagt ggagcgcgta gcgaaaacac ccattctggc
    51001 attaccgccg agacgaggcg gacgcacagt cggacgccag ccgccttcca tcgtttgatg
    51061 aaagacgaaa aggatccata aatggctgaa tgcttccagg ccgcgaacgg cgtcggcctg
    51121 gttgtaggga gcaatgagat gtagttcgcc gttggcgctt tttaccagac ctggctggcg
    51181 cggaacggcg aatttttctt tatagggcga gcgaataacg cctatttgct cgaactggaa
    51241 actgctcatt tcgccgtgat gttaagcgca gaaccgatac atacagcctg acgatagcaa
    51301 cctggcgtac cgctggtgac ttcgcagcta tgcagtaata ccgcattggc tttcattttc
    51361 gaggcgttga tttgcatccg cttacgtgcg gttggaatgc tcggcggaga gtcctgatta
    51421 gaggcctggc aagagtcgcc actgacttca ccgagatcgc ggaacggttt gccgactaat
    51481 tcttctgcat tggtataaat tcggaccggc gtggcgcgcg gcgctttcgg ttttgcaggc
    51541 tccgctttcg gctggggtgc agtgctttga acgggttcga caggggatct gcttaacatg
    51601 gaacagccgc ttagcatgag tgctactaaa cagatcggta aagcacgcat agtatttcct
    51661 caatgtatga tcaaaacgtc aatattgaat caggcgcttg taaaaatgac aagacgggca
    51721 agcgcccgtc ctgaatgata ttacaaattg tggaaacagc ctaaatatta ccagccttta
    51781 acagcgccgc cgttaaacac tttgtttgct gcttcgtaaa cttcgtcaga ctgataagcc
    51841 tgaacgaatt tcttcacgtt ttcggcgtct ttgttgtctt cacgcgttac gatcaggttt
    51901 acgtacgggg actctttatc ttcgacaaag ataccgtctt tcgctggagt caggccaatc
    51961 tggctggcat aggtggtatt gataactgcc agagcgattt gcgcgtcgtc cagagagcgc
    52021 ggcagctgcg gtgcttccag ttcaacaatt ttcagatttt ttgggttctc aacaacatca
    52081 agaacggtcg gcagcaggcc aacgccatct ttcagtttga tcaagcccac tttttgcagc
    52141 agcagcagtg aacgaccaag gttagttggg tcgtttggca cggcaacctg cgaaccatcc
    52201 tgcagttcat ccagtgattt gattttcttg gagtaaccag caatcggata aacaaatgtg
    52261 ttgcctactg cgaccagttt gtagccacga tctttcagtt gctgatcaag gtacggttta
    52321 tgctggaagg cgttggcgtc gatatcgcct ttgctcaatg cttcgtttgg cagaacatag
    52381 tcgttgaagg ttaccagctc aacgtccagg ccatatttgt ctttcgcaac tttctgcgcg
    52441 acttctgcaa cctgctgttc ggcaccaaca atcacgccga ctttaatgtg gtttggatct
    52501 ttttcatcct gaccgcagcc taccagtgcc agtgatccaa tcagggctcc cactgccgca
    52561 aaggttttga atttgaacgc cataccttat tccttcttca attatttatg ttgtgttgaa
    52621 cgttacttgc gagtgacagc ccggacgatg cggtcgcctg cgaactgaat taaataaacc
    52681 agaatgacca gcaataccag taccgtattc atcaccgtcg cgttgtagcc gatgtagcca
    52741 tactgatagc caatctgacc taaaccaccg gcaccgactg caccacccat cgcggaataa
    52801 ccgaccaggg taatcagggt aatagttgcc gcattcacca gacccggcaa cgcttccggt
    52861 aacagcactt tacggacgat ctgcattggc gtggcaccca ttgcgcggga ggcttcaatt
    52921 aacccggttg ggatctccag aagagcgttc tcgaccatac gggcaataaa cggtgctgca
    52981 ccaacggtta acggaacaat cgctgcctgc aatccaatcg atgtaccgac aataacgcgg
    53041 gtaaacggaa tcatccatac cagcaagata atgaacggga tggaacggaa aatgttcaca
    53101 atcgcagaaa tggtacgata caacttcgcg ttagcaataa tttgccccgg acgcgtgaca
    53161 taaagcagaa cgccaaccgg cagaccaatc acaaagccaa aaaaaccgga tacgaaggtc
    53221 attgccagcg tttcccatac gccacgaacc agcagccaca tcatcggctc agacataacc
    53281 cagtacctct acttttacat ggtgttcctg cagccaggca atggcggctt gcgtatcttg
    53341 ttgtgtgccg tgcatttcag tcagcataat gccgaactta acgccaccgg cgtaatccat
    53401 ctgcgcgcta ataatgttgt tgttgacgtt gaaacgacgc gcggtttcag aaagcagtgg
    53461 ggcatcgacc gattgaccgg taaactccag acgcagcatc ggcacgcagt cagtaaatgg
    53521 ctccgcttgc agacgttcct ggtaatcttc cgggatatcc agatgcaggg tcgactgaat
    53581 aaacttctgc gccagcggcg ttttcggatg cgagaacact tcacttaccg tgtcctgctc
    53641 gatcagttcg ccattgctga tgaccgccac gcaatcacaa atgcgcttca caacgtccat
    53701 ttcgtgagtg atcaacagaa tcgtcaaacc cagacggcgg ttgatgtctt tcagcagttc
    53761 gagaatagaa cgtgtcgttg ccgggtccag cgcgctggtg gcttcatcac acagcaatac
    53821 tttgggattg ctggctaatg cacgggcaat cgccacacgt tgtttctgcc caccggaaag
    53881 attcgacggg tagctatcat gcttatcgcc aagaccaacc aatgacagca attccgtcac
    53941 gcgacgtttg atctcgtctt tcggtgtgtt gtccagctcc agcggcagag ccacgttgcc
    54001 aaaaacagta cgcgaagaga gcaggttaaa atgctggaaa atcataccaa tctggcgacg
    54061 agctttggtc aactcggatt ctgacagcgt ggtcagttcc tggccatcga ccagcacgct
    54121 accctcggtt gggcgctcca gcaggtttac acaacgtata agcgtactct tacccgcgcc
    54181 tgaggcaccg ataacgccat aaatttgtcc agctggcaca tgcaggctga cgttgttcaa
    54241 cgcctggatg gtgcgggtgc cctggtggaa cactttggtg atattcgaaa gttttatcat
    54301 tgattattta ttatcgtcat taagttagtc gtggcatttc gaatgcctgg aacgggcaac
    54361 gccgtcaatg aaatggatgt taaggcatcc agacgtctaa atcaatcagg tttatgcgaa
    54421 gagcactttc ttgcaggtcg aaacatgcga tactagcgtc acatgcctta ttaaggagct
    54481 ataaaaggtg gcgaagagcg tacccgcaat ttttcttgac cgtgatggca ccattaatgt
    54541 cgatcacggt tatgtccatg agatcgacaa ctttgaattt atcgacggtg ttattgacgc
    54601 catgcgcgag ctaaaaaaaa tgggctttgc gctggtggta gtaaccaacc agtctggcat
    54661 tgctcgcggt aaatttactg aagcacagtt tgaaacgctg accgagtgga tggactggtc
    54721 gctggcggac cgcgatgtcg atctggatgg tatctattat tgcccgcatc atccgcaggg
    54781 tagtgttgaa gagtttcgcc aggtctgcga ctgccgcaaa ccacatccgg ggatgttttt
    54841 gtcagcacgc gattatttgc atattgatat ggccgcttct tatatggtgg gcgataaatt
    54901 agaagatatg caggcagcgg ctgcggcgag cgtgggaaca aaagtgctgg tgcgtacggg
    54961 taaacctatt acgcctgaag cagaaaacgc ggcggattgg gtgttaaata gcctggcaga
    55021 cctgccgcaa gcgataaaaa agcagcaaaa accggcgtaa tgattaaaag atgagcggtt
    55081 gaaataaaaa tgcatttttc cgcttgtctt cctgagac
//
LOCUS       DACADF010000010        53493 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.010, whole genome shotgun sequence.
ACCESSION   DACADF010000010 DACADF010000000
VERSION     DACADF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.010"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(405..491)
                     /locus_tag="ISB30_000599"
     tRNA            complement(405..491)
                     /locus_tag="ISB30_000599"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(455..457),aa:Leu,seq:gag)
     gene            complement(506..838)
                     /gene="secG"
                     /locus_tag="ISB30_000600"
     CDS             complement(506..838)
                     /gene="secG"
                     /locus_tag="ISB30_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP1988278.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1066..2403)
                     /gene="glmM"
                     /locus_tag="ISB30_000601"
     CDS             complement(1066..2403)
                     /gene="glmM"
                     /locus_tag="ISB30_000601"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP1988279.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2396..3244)
                     /gene="folP"
                     /locus_tag="ISB30_000602"
     CDS             complement(2396..3244)
                     /gene="folP"
                     /locus_tag="ISB30_000602"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAP1988280.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLLDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVCVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3334..5268)
                     /gene="ftsH"
                     /locus_tag="ISB30_000603"
     CDS             complement(3334..5268)
                     /gene="ftsH"
                     /locus_tag="ISB30_000603"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP1988281.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5368..5997)
                     /gene="rlmE"
                     /locus_tag="ISB30_000604"
     CDS             complement(5368..5997)
                     /gene="rlmE"
                     /locus_tag="ISB30_000604"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAP1988282.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6123..6416
                     /gene="yhbY"
                     /locus_tag="ISB30_000605"
     CDS             6123..6416
                     /gene="yhbY"
                     /locus_tag="ISB30_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP1988283.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6572..7048)
                     /gene="greA"
                     /locus_tag="ISB30_000606"
     CDS             complement(6572..7048)
                     /gene="greA"
                     /locus_tag="ISB30_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP1988284.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7296..8729
                     /gene="dacB"
                     /locus_tag="ISB30_000607"
     CDS             7296..8729
                     /gene="dacB"
                     /locus_tag="ISB30_000607"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAP1988285.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVTTLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGIAWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8770..9942)
                     /gene="cgtA"
                     /locus_tag="ISB30_000608"
     CDS             complement(8770..9942)
                     /gene="cgtA"
                     /locus_tag="ISB30_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAP1988286.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9958..10923)
                     /locus_tag="ISB30_000609"
     CDS             complement(9958..10923)
                     /locus_tag="ISB30_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP1988287.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLANPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11050..11307)
                     /gene="rpmA"
                     /locus_tag="ISB30_000610"
     CDS             complement(11050..11307)
                     /gene="rpmA"
                     /locus_tag="ISB30_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP1988288.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11328..11639)
                     /gene="rplU"
                     /locus_tag="ISB30_000611"
     CDS             complement(11328..11639)
                     /gene="rplU"
                     /locus_tag="ISB30_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP1988289.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11898..12869
                     /gene="ispB"
                     /locus_tag="ISB30_000612"
     CDS             11898..12869
                     /gene="ispB"
                     /locus_tag="ISB30_000612"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAP1988290.1"
                     /translation="MNLEKINELTAQDMAGVNASILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13102..13380
                     /gene="sfsB"
                     /locus_tag="ISB30_000613"
     CDS             13102..13380
                     /gene="sfsB"
                     /locus_tag="ISB30_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAP1988291.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13428..14687)
                     /gene="murA"
                     /locus_tag="ISB30_000614"
     CDS             complement(13428..14687)
                     /gene="murA"
                     /locus_tag="ISB30_000614"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP1988292.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14742..14996)
                     /gene="ibaG"
                     /locus_tag="ISB30_000615"
     CDS             complement(14742..14996)
                     /gene="ibaG"
                     /locus_tag="ISB30_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAP1988293.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15156..15449)
                     /gene="mlaB"
                     /locus_tag="ISB30_000616"
     CDS             complement(15156..15449)
                     /gene="mlaB"
                     /locus_tag="ISB30_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAP1988294.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15449..16084)
                     /gene="mlaC"
                     /locus_tag="ISB30_000617"
     CDS             complement(15449..16084)
                     /gene="mlaC"
                     /locus_tag="ISB30_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAP1988295.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16103..16654)
                     /gene="mlaD"
                     /locus_tag="ISB30_000618"
     CDS             complement(16103..16654)
                     /gene="mlaD"
                     /locus_tag="ISB30_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAP1988296.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16659..17441)
                     /gene="mlaE"
                     /locus_tag="ISB30_000619"
     CDS             complement(16659..17441)
                     /gene="mlaE"
                     /locus_tag="ISB30_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAP1988297.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17449..18258)
                     /gene="mlaF"
                     /locus_tag="ISB30_000620"
     CDS             complement(17449..18258)
                     /gene="mlaF"
                     /locus_tag="ISB30_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAP1988298.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18468..19445
                     /locus_tag="ISB30_000621"
     CDS             18468..19445
                     /locus_tag="ISB30_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAP1988299.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19459..20445
                     /gene="kdsD"
                     /locus_tag="ISB30_000622"
     CDS             19459..20445
                     /gene="kdsD"
                     /locus_tag="ISB30_000622"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAP1988300.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20466..21032
                     /gene="kdsC"
                     /locus_tag="ISB30_000623"
     CDS             20466..21032
                     /gene="kdsC"
                     /locus_tag="ISB30_000623"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAP1988301.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21029..21604
                     /gene="lptC"
                     /locus_tag="ISB30_000624"
     CDS             21029..21604
                     /gene="lptC"
                     /locus_tag="ISB30_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAP1988302.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21573..22130
                     /gene="lptA"
                     /locus_tag="ISB30_000625"
     CDS             21573..22130
                     /gene="lptA"
                     /locus_tag="ISB30_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAP1988303.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22137..22862
                     /gene="lptB"
                     /locus_tag="ISB30_000626"
     CDS             22137..22862
                     /gene="lptB"
                     /locus_tag="ISB30_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAP1988304.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            22910..24343
                     /gene="rpoN"
                     /locus_tag="ISB30_000627"
     CDS             22910..24343
                     /gene="rpoN"
                     /locus_tag="ISB30_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP1988305.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24366..24653
                     /gene="hpf"
                     /locus_tag="ISB30_000628"
     CDS             24366..24653
                     /gene="hpf"
                     /locus_tag="ISB30_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAP1988306.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24771..25262
                     /gene="ptsN"
                     /locus_tag="ISB30_000629"
     CDS             24771..25262
                     /gene="ptsN"
                     /locus_tag="ISB30_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAP1988307.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25308..26162
                     /gene="rapZ"
                     /locus_tag="ISB30_000630"
     CDS             25308..26162
                     /gene="rapZ"
                     /locus_tag="ISB30_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP1988308.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26159..26431
                     /gene="npr"
                     /locus_tag="ISB30_000631"
     CDS             26159..26431
                     /gene="npr"
                     /locus_tag="ISB30_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAP1988309.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26644..27276
                     /gene="yrbL"
                     /locus_tag="ISB30_000632"
     CDS             26644..27276
                     /gene="yrbL"
                     /locus_tag="ISB30_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAP1988310.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27273..28001)
                     /gene="mtgA"
                     /locus_tag="ISB30_000633"
     CDS             complement(27273..28001)
                     /gene="mtgA"
                     /locus_tag="ISB30_000633"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAP1988311.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(27998..28651)
                     /gene="elbB"
                     /locus_tag="ISB30_000634"
     CDS             complement(27998..28651)
                     /gene="elbB"
                     /locus_tag="ISB30_000634"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAP1988312.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28881..31217)
                     /gene="arcB"
                     /locus_tag="ISB30_000635"
     CDS             complement(28881..31217)
                     /gene="arcB"
                     /locus_tag="ISB30_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAP1988313.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31313..32242)
                     /locus_tag="ISB30_000636"
     CDS             complement(31313..32242)
                     /locus_tag="ISB30_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP1988314.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            32917..37377
                     /gene="gltB"
                     /locus_tag="ISB30_000637"
     CDS             32917..37377
                     /gene="gltB"
                     /locus_tag="ISB30_000637"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAP1988315.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37390..38808
                     /locus_tag="ISB30_000638"
     CDS             37390..38808
                     /locus_tag="ISB30_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HAP1988316.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAIVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(38876..39340)
                     /locus_tag="ISB30_000639"
     CDS             complement(38876..39340)
                     /locus_tag="ISB30_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAP1988317.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(39337..40212)
                     /locus_tag="ISB30_000640"
     CDS             complement(39337..40212)
                     /locus_tag="ISB30_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAP1988318.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEIL"
     gene            complement(40209..40898)
                     /locus_tag="ISB30_000641"
     CDS             complement(40209..40898)
                     /locus_tag="ISB30_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP1988319.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(40946..42436)
                     /gene="nanT"
                     /locus_tag="ISB30_000642"
     CDS             complement(40946..42436)
                     /gene="nanT"
                     /locus_tag="ISB30_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAP1988320.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGISGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPILGALIAQRLDLGTALGSLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(42545..43438)
                     /gene="nanA"
                     /locus_tag="ISB30_000643"
     CDS             complement(42545..43438)
                     /gene="nanA"
                     /locus_tag="ISB30_000643"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAP1988321.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(43560..44351)
                     /gene="nanR"
                     /locus_tag="ISB30_000644"
     CDS             complement(43560..44351)
                     /gene="nanR"
                     /locus_tag="ISB30_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAP1988322.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(44459..44956)
                     /gene="sspB"
                     /locus_tag="ISB30_000645"
     CDS             complement(44459..44956)
                     /gene="sspB"
                     /locus_tag="ISB30_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAP1988323.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(44962..45600)
                     /gene="sspA"
                     /locus_tag="ISB30_000646"
     CDS             complement(44962..45600)
                     /gene="sspA"
                     /locus_tag="ISB30_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAP1988324.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(45995..46387)
                     /gene="rpsI"
                     /locus_tag="ISB30_000647"
     CDS             complement(45995..46387)
                     /gene="rpsI"
                     /locus_tag="ISB30_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP1988325.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(46403..46831)
                     /gene="rplM"
                     /locus_tag="ISB30_000648"
     CDS             complement(46403..46831)
                     /gene="rplM"
                     /locus_tag="ISB30_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP1988326.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(47051..48178)
                     /locus_tag="ISB30_000649"
     CDS             complement(47051..48178)
                     /locus_tag="ISB30_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAP1988327.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYKELVNSTP
                     PAPRTSGLMARVGKLWGKREDTKHMPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            48372..48770
                     /locus_tag="ISB30_000650"
     CDS             48372..48770
                     /locus_tag="ISB30_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAP1988328.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            48924..50291
                     /gene="degQ"
                     /locus_tag="ISB30_000651"
     CDS             48924..50291
                     /gene="degQ"
                     /locus_tag="ISB30_000651"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAP1988329.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIIRGNESL
                     YLLMR"
     gene            50381..51448
                     /gene="degS"
                     /locus_tag="ISB30_000652"
     CDS             50381..51448
                     /gene="degS"
                     /locus_tag="ISB30_000652"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAP1988330.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(51511..52449)
                     /gene="mdh"
                     /locus_tag="ISB30_000653"
     CDS             complement(51511..52449)
                     /gene="mdh"
                     /locus_tag="ISB30_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAP1988331.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            52884..53354
                     /gene="argR"
                     /locus_tag="ISB30_000654"
     CDS             52884..53354
                     /gene="argR"
                     /locus_tag="ISB30_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAP1988332.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
BASE COUNT        13210 a        14448 c        13298 g        12537 t
ORIGIN      
        1 ttatatcaga catgtttttc aactctaatc catatcagac cgaaacactg ctagattttt
       61 tctttttaaa tgatgcggcc attgcgttct cagtaattaa ataacattat caatgcgtct
      121 ctaaacatta cgcagaacgt agccatcagt agttatactg acaggggcag gatggtttat
      181 cgagaagtaa acatatccgt attcgtaccg gaaatggagt tgacacaata aagtgccaat
      241 tatgtcagca gaagggaaaa gcttataact aaggcatacc ggcaccatcg tttcaaggta
      301 ccagctacga gtaaaacaac tggacgagat acagatacct gacaaccatt cctcagacca
      361 ggaccaaaac gaaaaaagac gcttttcagc gtctttctgg aatttggtac cgaggacggg
      421 acttgaaccc gtaagcccta ttgggcacta ccacctcaag gtagcgtgtc taccaattcc
      481 accacctcgg cacggatact actttttagt tcgggatatc gctggtcggc ttagccggag
      541 cagctggctg agtttgttcg gttttcgccg gcgcactcag gttttcccat tcgctacctt
      601 tattggtttt gttgctattg atgttaccca gcaccagact gatgatgaag aataacgttg
      661 ccagcagcgc cgtcatgcga gtcatgaagt taccagaacc acttgaacca aacagcgtag
      721 cggaagcgcc tgctccgaag gaggctccca tatcagcgcc tttaccttgc tgcagcatga
      781 tcagaccaac aaggccaatt gccacaataa ggaaaactac taaaagagct tcgtacataa
      841 tcaacctgtt ccttgcggag ttgccgcgta ccaatgcttc aaccaataaa gcgggaatat
      901 ttaacctttc ccactgaagc gggtgtgaat actaaccaaa gcgaatgacc ttcgcaaggg
      961 caattttatt gcattgtatc aactgcggaa aaaaacagca aaaccgatgt gttcgctgag
     1021 aaaaaaggcg acaatcgccg cctttttagc cagccattta tcgttttaaa cggcttttac
     1081 tgcatcggcg atgcggtgtg caaattcagt cacctgcgct tcgtcttcgc cttccaccat
     1141 cacgcgaatt aacggttcgg tgccggattt acgcaacagc acacgtccac ggctacccag
     1201 cgccgcttca acttctgcgg tcacagcttt gactgattca tgctccaacg gatcgccgct
     1261 acctgcggtg taacgcacat taaccagaat ctgcgggaac attttcatgc cgctgcaaag
     1321 gtcgtgcagg ctcatatggt tacgcgccat cgccgccagc acctgcaagc cagcaacaat
     1381 gccgtcaccg gtagtggttt tatccagcag gatcacatga ccggagttct ctgcaccgat
     1441 acgccagcct ttctcctgca ttttttccag tacgtagcgg tcacccactt tcgcgcgcgc
     1501 aaatggaata cctaactgtt tcagcgccag ttcaagcccc atgttgctca tcaatgtacc
     1561 cacagcacca ccacgcagct ggccctgacg aagaccttca cgcgcgatga tatacatgat
     1621 ctgatcgcca tcgactttat tgccttcatg gtcaaccata atcacgcgat cgccgtcgcc
     1681 gtcgaaggca ataccgagat ccgctttttc agccagcaca cgagcctgga gcgcgcgaac
     1741 gtcggtagcc cccacttcgg cattgatatt tacaccgttc ggctcacaac cgatagcgat
     1801 aacgttcgcc cccagttcac gcagcacgtt cggtgcgatg tgataagtcg caccgtttgc
     1861 acaatccacc acaatcttca gttcactgag gctaagttcg ttcgggaacg tggctttgca
     1921 aaactcgata taacgacccg cggcatcaac gatacggctg gctttaccca gttctgccga
     1981 atcaacacag ctgatctctt tttccatttc cgcttcgatg gcttcttcaa ctgcatccgg
     2041 cagcttagta ccgtcaatag aaaagaattt aatgccgtta tcgtagaatg ggttatgcga
     2101 tgcagaaatt acaattccag cctctgcgcg gaaggtacgc gtcagataag ccacggccgg
     2161 tgttggcatc gggccagtga agagcgcgga aagaccggca gccgcaagac ccgcttccag
     2221 tgctgactcc agcatatagc cagaaatacg cgtgtcttta ccaataataa tcttacgaga
     2281 gccgtggcgc gccagcactt tacccgcggc ccaacccagc ttaagcacaa aatcaggtgt
     2341 gatcggcgca tcccctacac gaccacgaat cccatcggta ccgaaatatt tacgattact
     2401 catagcgttt gttttccttt gcagacagag tggcttccac cacccgcatc gcttctacgg
     2461 tttctttgac gtcatgaaca cgaatgatgt gcgcgccttg cattgcggca atgaccgcac
     2521 aggccagact accgctcagg cgctcggacg gccccacgtt cagcaattgc ccaatcatcg
     2581 attttcgtga catacccacc aacagcggca ggttgaaatg gtgaaattca gccaggcgcg
     2641 ccagtaatga atagttatgg gagagatttt taccgaaacc gaatccgggg tcgagcaaca
     2701 atttctcttt tgcgataccc gcctgctcgc aacgtgctat ttgctcaata aagtagcgat
     2761 tcacttctgc aaagacatcg tcatacttcg gcgcttcctg catggttttt ggatttccct
     2821 gcatatgcat cagacaaacc ggtaaaccgg tttctgcagc cgcctccaga gcaccaggtt
     2881 cggaaaggga gcggatatcg ttaataatgt gagcgccaac tttcgctgac tcacggatga
     2941 cttctggttt ggatgtatct accgagatcc agacttcgaa gcgttgagca attgcctcaa
     3001 ccacaggaat aacacgttgc aactcttctt caacgcaaac ttccgccgcc cctgggcgcg
     3061 tggactcgcc accaacgtca atgatcgtcg cgccagcgtt gatcatcaga ttcgcatgtt
     3121 tcaccgcatc taacagcgag ttatgcgtgc caccatccga aaaggaatca ggcgtgacgt
     3181 tgaggatccc cattacgtga ggatggctaa ggtccagtga agtaccctgg gcaaagagtt
     3241 tcatgatgtt atccctggta tgaattgata agaaaaaacc ccggagcacg ccccggggtt
     3301 ttcggtacaa atacagtcat ctgatacggg aacttacttg tcgcctaact gctctgacat
     3361 ggtgttaccc gggttcggcg tacgcggttc atcaaccgga cgaggagcct ttggactacc
     3421 attgtcgcca gaattgttag aagcgcctgg ttcttcccag cccgctggcg gacgtacatc
     3481 gcgacgtgcc atcaggtcat caatctgcgg tgcgtcgata gtctcatatt tcatgagagc
     3541 atctttcatc gcatgcagaa tatccatatt gtcggtcaga agctgacgcg cacgattata
     3601 gttacgctca atcagtgctt tcacttcctg gtcgatgata cgtgcagttt catcggacat
     3661 atgtttcgct ttcgctacgc tacggccgag gaacacttca ccttcttctt ccgcgtacag
     3721 cagtggaccc aatttctcag agaagcccca ctgggtcacc atgttacgtg ccaggttggt
     3781 cgcaacttta atatcgttgg acgccccggt agaaacatgt tccggtccgt agatgatctc
     3841 ttccgccaga cgaccaccgt acagcgtgga gatctgactt tccagtttct gacggctggc
     3901 gctgattgcg tcgccctcag gcaagaagaa agtcacaccc agcgcacgac cgcgtgggat
     3961 aatcgtcact ttgtgcaccg gatcgtgttc cggcaccagg cgaccgataa tcgcatgacc
     4021 cgcctcgtgg taagccgtcg attctttctg cgcttccgtc atcaccatgg agcgacgttc
     4081 cgcacccatc atgattttgt ctttcgcttt ctcgaactca accatcgaaa caacgcgttt
     4141 gttgccacga gcagcgaaca gtgccgcttc gttcaccagg ttcgccaggt cagcaccgga
     4201 gaaaccagga gtaccacggg caatgattgc cgcgtcgata tcgggtgcca atggtacgcg
     4261 acgcatgtga actttcagga tctgctcacg accgcgaaca tctggcaagc caaccacaac
     4321 ctgacggtcg aaacggccag gacgcagcag ggctgggtcg agaacgtccg gacggttagt
     4381 cgcagcgata acgatgatac cttcgttacc ttcaaaacca tccatctcaa ccagcatctg
     4441 gttcagagtc tgttcacgtt catcgtgacc accgcccaag cctgcgccgc gctggcggcc
     4501 tacggcgtcg atttcatcga taaagatgat gcacggtgcc gctttcttcg cctgttcgaa
     4561 catgtcacga acacgggatg cacccacacc gacgaacatt tctacgaagt cagaaccgga
     4621 gatagtaaag aacggaactt tcgcttcgcc tgcaattgct ttcgccagca acgttttacc
     4681 ggtacccgga ggacctacca tcaggacgcc tttcgggatc ttaccgccga gtttctggaa
     4741 gcggctcggc tcacgcagat actcaaccag ttcagcaact tcttcttttg cttcgtcgca
     4801 gcccgcaacg tcagcaaagg tggttttgat ctgatcttcc gtcagcatgc gcgctttgct
     4861 cttaccaaac gacatggcac ctttgccacc gccgccctgc atttgacgca tgaagaagat
     4921 ccagacacca atcagcaaca gcatcgggaa ccaggagatg aagatagaag ccagcaggct
     4981 tggttcttca ggcggttcac cgacaacctt gacgttcttg gtcaacaggt tatccagtaa
     5041 tttcggatcc tgaaccggaa tgtaagtggt ataacggtta ctatctttct tggtaacgtt
     5101 gatttcacgt ccgttgatac gcgcttcacg aacctggtcg ttattgacct cttgtaggaa
     5161 ggtagagtaa tccaccttac ggccattaga ctcgctgggc ccaaagctct ggaatactga
     5221 catcagcaca acggcaatga ccagccagag tattaggttt ttcgccatgt cactcaaggg
     5281 attaacctct tattacaact gtgttaaaaa cagcgtcagg atactctata tccagcatct
     5341 ttcaaacttt cgtctgaaat ctcccggtta gggtttacgc ccggtcgcta caatatacac
     5401 ttcccgcgaa cgtgcacgag aagagtccgg cttacgaact ttgaccttcg taaacaggga
     5461 gcgaatttcc cttagatact catcgaaacc ttcgccctgg aacaccttca ctacaaaact
     5521 gccacctggc gctaatacat cacgacacat ttctagcgcc agttccacca gatacatggc
     5581 acgggggata tccaccgccg gtgttccgct catgtttggt gccatatcgg acatgacaac
     5641 ctggactttg ctgtcgccaa cgcgctccag cagtgctttc atcaccagtt catcacgaaa
     5701 atcgccctga agaaagtcca caccaacgat aggatccata ggtaaaagat cgcaagcgat
     5761 gatgcggcct ttgccgccaa tttgggtgac cacatattgt gaccaaccac ccggagcagc
     5821 accaaggtcg acaaccgtca ttcccggttt aaagagtttg tcactttgct gtatttcatc
     5881 aagtttaaac caggcacggg aacgtaaccc ctttttctgt gcctgttgaa catatttatc
     5941 gctaaagtgt tcctgaagcc agcggctgga gctggcagaa cgcttcttac ctgtcattta
     6001 actttcccat ggggataact catcgtaacc aattgcgtaa atttttacgc gcctatttgg
     6061 tgatatatgg gagatggcgg tagaatgacc cgttttcaat cccaacgtaa gcaaaaaata
     6121 cgatgaatct gagtactaaa caaaaacagc acctgaaagg tctggcacat ccgctcaagc
     6181 cagttgttct gcttggcagt aatggtttga ccgaaggggt gctggccgag attgaacaag
     6241 cgttagagca ccatgaactc atcaaggtga aaatcgccac cgaagatcgc gaaactaaaa
     6301 ccttgatcgt ggaagctatc gtgcgcgaaa ccggcgcctg taatgtacag gtcatcggta
     6361 aaacgctggt gctttatcgc ccaactaaag aacgtaaaat ctcgctgcca cgctaagatt
     6421 atcctaaagt tacacacatt cgctgtgtaa aacgaggggt tttccgcagg caggagagca
     6481 aaatgccacg ctctgttcgt tgataaaagg ccgcatagcg gcctttttcc tttctttaca
     6541 atacatcaac atcttgagta ttgggtaatt cttacaggta ttccacctta attacttcaa
     6601 attctacttc gccgcccggc gttttgatga ccacaacatc atcttcttct ttgccgatca
     6661 ggccacgagc aataggcgag tttacagaaa tcaggttttg tttaaagtca gcttcgtcat
     6721 cgccaacgat gcgataagtc tgttcttcgt cagaatcgag attcagcacc gttacggtag
     6781 caccaaaaat aacgcgccca ttgttgggca ttttggtgac atcaatcacc tgcgcgttcg
     6841 acagcttggc ttcgatgtct ttgatacggc cttcgcagaa gccctgctgt tcacgagctg
     6901 cgtggtattc ggcgttttct ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa
     6961 tgatttcagg acggcgcaca gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc
     7021 cgcgtaaggt catcggaata gcttgcattt gttatacctc ttgaatattc ctgatggggc
     7081 aagtcttcac ccaccccggc tgttcggcct gcccggcata actcctcacc gggaccagaa
     7141 gcaaaaaaat accgacccgg gtacaagtcc caggtcagct acaattcaca ttttgatagt
     7201 cattttaccc tggagttccc gaagggtcat cgtttacttt atagggcgtt gcgccgtagt
     7261 atgacggctc gattccaggt tgttagcgcg agattatgcg attttccaga tttatcatcg
     7321 gattgaccag ctgtatagcg ttcagtgttc aggccgcaaa tgttgatgag tacattactc
     7381 aactccccgc aggtgccaac cttgccctga tggtgcaaaa agtcggcgcg tcggcccccg
     7441 ctattgatta ccacagtcag cagatggcgc tacccgccag tacgcagaaa gtgattactg
     7501 cgctggcggc gttgattcaa ctcggccccg attttcgttt taccacgacg cttgaaacca
     7561 aaggcaatgt ggaaaacggc gtacttaagg gtgacttagt tgcgcgattt ggtgccgatc
     7621 cgacgttaaa acgtcaggat attcgcaata tggtcacgac tttgaaaaaa tctggcgtca
     7681 accaaatcga tggcaatgtg ttgatagata cctccatttt cgccagccac gataaagccc
     7741 ccggctggcc atggaatgac atgacacaat gctttagcgc accgcctgcc gccgccatag
     7801 ttgaccgcaa ctgtttctcc gtctcgctct acagtgcccc aaaacctggt gatatggctt
     7861 ttatacgcgt ggcatcttat taccccgtta cgatgttcag ccaggtacgc accctccccc
     7921 gtggttctgc cgaagcgcaa tactgcgaac tggatgtcgt accaggtgac ctgaaccgct
     7981 ttacgctaac gggatgcctg ccacaacgtt ctgagccgct cccgttggct tttgccgtgc
     8041 aggatggtgc cagctatgcc ggggcaattc tgaaagatga gttaaaacag gcgggtatcg
     8101 cctggagcgg aacactgctg cgccagactc aggttaacga acctggaacg gtagttgcca
     8161 gtaaacagtc ggccccgctg cacgatctgc ttaagattat gctgaaaaag tcggacaaca
     8221 tgattgccga tacggttttc cgcatgatcg gccatgcgcg cttcaatgtg cctggaacat
     8281 ggcgggccgg atcggacgcc gtgcgtcaga tcctgcgcca gcaagccggt gtcgatatcg
     8341 gaaacaccat tattgccgat ggttcagggc tttcgcggca taacctgatt gcccccgcca
     8401 ccatgatgca ggtgctgcaa tatattgccc aacacgacaa tgaacttaac tttatctcca
     8461 tgctgccgct ggcgggctat gacggctctt tgcagtaccg tgcaggtctg catcaggcgg
     8521 gcgtggatgg aaaagtctca gcgaaaaccg gttcgttgca gggggtatat aacctggcgg
     8581 gattcattac cacagcgagc gggcaacgaa tggcgtttgt gcaatatctt tctggctatg
     8641 cagtagaacc ggcggatcag cgtaatcgcc gtattccgtt agtgcgtttt gaaagccgtt
     8701 tgtataaaga tatttatcag aataattagc ctaaagagaa accccggcac atggccgggg
     8761 ttttgctgat taacgcttgt aaatgaactc aacgccttct tcgtcgtctt cgtcccagtc
     8821 gtcatcccag tcctcatcat cctcttcagc aatctcttca agctgctgac ggtgataatc
     8881 atcccacatg aattcgactt tctctggctg tttcgcttct tcagcctgca cgaccgggtt
     8941 ttcaatgata aaggtcatca catcccagca gagatctttc acgcccagtc cgctcgccgc
     9001 agagatcaga taatatttat cttcccagcc cagcgcttca gcgatcgctt tcgctttctc
     9061 ttcggcttct gccttatcca gcagatcgat cttgttgaaa actaaccaac gcggtttcgc
     9121 cgccagatcc tggctgtatt tttccagctc actgataata atacgcgcgt tttcaaccgg
     9181 atcggtgccg tcaatcggat cgatatcgat gaggtgcaac aggacgcggc aacgttccag
     9241 gtgcttcagg aagcgaatgc ccagacctgc gccttctgca gcgccttcaa tcagccctgg
     9301 aatatcggca acaacgaagc tcttttcgtt gtccattcgc accacgccca ggcttggtac
     9361 cagagtggta aacggataat ccgccacttt cggtttagcc gccgatacag cacgaataaa
     9421 ggtcgattta cccgcgtttg gcatccccaa cataccgacg tcagccagca gcatcagctc
     9481 cagcagcagc tcacgcttat cgcccggcgt accgttagtt ttctgccgtg gtgtacggtt
     9541 aactgacgat ttgaaacgag tgttgcccag accgtgccag ccgcccttag caaccagcag
     9601 acgctgaccg tgtttggtca tatcacccat ggtttcacca gtaccctggt cgattacacg
     9661 cgtacctacc ggcactttaa tcgtgacgtc tttaccgcgc ttacctgtac agtcgcggct
     9721 tgcaccgttt tgaccgcgct ctgcacggaa agatttttca aaacgataat cgataagcgt
     9781 gttcaggttc tcgtcggctt ccatccatac gtcaccaccg tcaccgccgt cgccgccatc
     9841 cgggccgcct ttcggaatat acttttcgcg gcggaagctc acgcaaccat taccgccatc
     9901 acctgcaacg accagaatcg atgcttcatc aacaaacttc attttattct ccgtaaatca
     9961 ttcgcctgcg cggggttgcg ataccaccgt tgtatgctta cgtaatccgc cccaaatacg
    10021 atgaccaatg gcggaataca tcgcgcccgc aaccacgaca aacgcaccga gataacctaa
    10081 aaggtttaac atcggtctgg cgaagaaatc gggccaggcc agtgataaaa gatctgaaaa
    10141 aaacagcgta aacagtgggg tgagcgtgat gatcgcgctc acctgcgctg cctgccagcg
    10201 agccatcgct tccgccaggg cgccatatcc taccaatgta ttcagtccgc aaaaaattaa
    10261 acaggcgagc tgccagtggc taagctgcgc tatcactcca ggatttgcca gagggaagag
    10321 cgcaattgta cataaagtgt acagcaaaaa caggatctgc ggtgaggcca gccgacgcaa
    10381 taaaaccttt tgcgccacgc cataactcac ccaaaccgtc gccgcaccga ccccaaagat
    10441 aactccccag gtgtaatcgg tgagctttgt aaatatctcg accagactgg tgttaaaaaa
    10501 catcaccagg ccgctcagga gcatcaatgc ccctacaacc tgagtactgc gcattttctc
    10561 tttcaggata aatacgctgg caaccatcat gccaactggc gagagttgcc caatcacctg
    10621 cgaagcggtc ggactcaggt attgcaagga tgagctgaac aggatgaagt tcccaaacag
    10681 cccggcggtc gccactgcta acaaaatcaa ccagcgtggc ttacgaaaca cgcgtaatgg
    10741 cggcaaccgc ttcttcaccg caagaatggc accaaggcca atactcgcca tcaagaaacg
    10801 gtaaaacacg attgtcggag gttccatcac ctccagcacc tgcttcattg cgattggcaa
    10861 cgccccccag caaattgctg tggtgagcgc caaaagaatg ccaatgcctg cctgctgctt
    10921 cataccgtat tccctgcaaa gcgcatatgc ggattaacgt cgcaccatgc gcatttttac
    10981 cggttatcga atgtaaaaag ccccgcaacg tgttgcgggg ctttcatccg ttaccgggac
    11041 gcgaaaaact tattcagctt cgatgctgat aaatttacgg tttttcgggc ctttaacttc
    11101 gaatttcact ttaccgtctg ctttagcaaa cagagtgtgg tcacgaccgc aacctacgtt
    11161 agcgccagcg tggaatttgg taccacgttg acgaacgatg atgctacccg ccagaacgga
    11221 ttcaccaccg aaacgcttaa cgcccaggcg tttagcttct gaatcgcgac cgttacgtgt
    11281 ggagccgcca gcctttttat gtgccatttg aaatctctcc tcaggtctta ggcgctgatg
    11341 ccagtaattt tcacatcagt gaaccactga cgatggccct gctgcttacg atagtgttta
    11401 cgacgacgaa acttaacgat tttaactttc tcgccacgac cgtgagcaac aacttcagct
    11461 ttgattacgc cgccatcaac gaaaggaacg ccgattttga cttcttcacc gtttgcgatc
    11521 atcagcactt cagcgaactc aacagtttcg ccagttgcga tgtccagctt ttccaggcga
    11581 acggtctgac cttcgcttac tcggtgttgt ttaccaccac tttggaaaac cgcgtacata
    11641 taaaactccg cttccgcgca caccttttca atgattcaga gtgcgctata aatattcaca
    11701 atagggcgcg aatattacgc aaaacgcacg cctttgacaa gtgctacagt caatacacga
    11761 agaaaaaaaa cacaacttgt acggtaacgt ttatctgtgc cattttttca gtacaatcac
    11821 cctatattcc taaccataaa ccctaagttg cctttgttca cagtaaggta atcggggcga
    11881 aaagcccggc ttttgcgatg aatttagaaa aaatcaatga gttaaccgcg caagatatgg
    11941 cgggtgttaa tgcgtcaatc cttgagcagc ttaattccga cgtccaactg atcaatcagt
    12001 taggctatta catcgtcagc ggcggcggta aacgtattcg tccgatgatt gctgtactgg
    12061 ctgcacgagc tgttggctat gagggaaatg cgcatgtcac cattgctgcc ctgatcgagt
    12121 ttatccacac ggcgactctg ctacacgacg acgttgtgga tgaatcagat atgcgcaggg
    12181 gtaaagctac cgccaacgcc gcatttggca atgccgccag cgtgctggta ggcgatttta
    12241 tttatacccg cgcgttccag atgatgacca gcctcggttc actcaaagtg ctggaagtca
    12301 tgtcagaagc cgtaaacgtc atcgcagaag gtgaagttct gcaactgatg aacgttaacg
    12361 atccggacat cactgaagaa aactacatgc gcgttatcta cagcaagact gcgcgtctgt
    12421 ttgaggctgc cgcgcagtgt tccgggattc tggctggctg tacgccggag gaggagaaag
    12481 gcctacaaga ttatgggcgc tatctcggca ctgctttcca gttgatcgac gatttactcg
    12541 attacaatgc cgatggcgaa cagttaggta aaaacgtcgg cgacgatctg aacgaaggta
    12601 aaccgacgct gccgctgctg catgcgatgc atcatggcac accagaacag gcacagatga
    12661 ttcgtaccgc catcgaacag ggtaacggtc gccatcttct ggaaccggtt ctggaagcaa
    12721 tgaacgcttg tggatctctt gaatggacgc gtcagcgtgc cgaggaagaa gcagacaaag
    12781 ccatcgcagc gttacaggtg ctcccggaca ccccgtggag agaagcactt atcggcctcg
    12841 cgcacatcgc tgttcaacgc gatcgttaat cccctcccct catcccgcgc agtgcgcggg
    12901 atgattccag taaattccat aaaacactta ttcagctcta taactcctga aaaacagggc
    12961 tgtgtatctt tatatattct gaatattcac actctttaca ggaacttttt agagcaatag
    13021 gccatcagga gtatagtgat gctcgacaga aacagaagaa gtattctgaa tgaaagcgac
    13081 taatttaagg agtgaggaaa gatggaaagt aatttcattg actggcatcc cgctgacatc
    13141 attgcaggtt tgcgcaagaa gggaacgtca atggcggcgg agtctcgcag aaatggtttg
    13201 agttcctcaa cgctggcgaa cgcattatca cgcccatggc cgaaaggaga gatgattatt
    13261 gcgaaagccc tgggaactga cccctgggtt atctggccat cacgctacca tgatccgcag
    13321 acccatgagt ttatcgacag aacgcagttg atgcgtagct acactaaacc gaaaaaatga
    13381 gtggtctggc ggtagccccg cgaacggggc tgccagctct cagacaatta ctcgcctttc
    13441 acacgctcaa tatttgcacc taaagcgcgt agtttgtctt caatgcgttc gtagccacga
    13501 tcgatgtgat aaatacgatc aaccaccgtc gtcccttccg caatacagcc agccagcacc
    13561 aggcttgctg atgcacgcag atcggttgcc ataacctgtg cgccagaaag tttttcaaca
    13621 ccgtgacaaa taacggtatt gctttcgatt tcggcgtgcg cgcccatacg gctcagctct
    13681 ggcacatgca taaagcggtt ttcaaagacc gtttcggtga tgaatccggt cccttctgcc
    13741 accaggttca acagcgtgaa ctgggcctgc atatcggtcg ggaatgccgg atgcggcgcg
    13801 gtacgtacgt taacagcctt cggacgtttg ccatgcatat ccaggctaat ccagtcttcg
    13861 ccgacttcga tgtccgctcc agcgtcacgc agtttcgcca gcacggcgtc cagagtatct
    13921 ggctgcgcgt tacggcagat aattttgccg cgagagatcg ccgccgccac caggaaagta
    13981 ccggtttcga tacgatccgg cagcacgcga tagacaccgc cgcctaaacg ttccacacct
    14041 tcgatgacga tacgatcggt gccctgaccg ctaattttcg cacccagcgt aatcaggaag
    14101 ttcgcggtat cgacgatttc cggttcacgc gctgcgtttt caataatcgt ggtgccttcc
    14161 gccagggttg cagcacacat gatggtcacc gttgcgccaa cgctgacttt atccatcacg
    14221 atatgtgcgc ctttcagacg accatcgacg gaagctttaa cgtaaccttc ttccagtttg
    14281 atggtcgcgc ctaattgttc gaggccagaa atgtgtagat caaccggacg tgcgccgatc
    14341 gtacaaccgc caggcagtga aacttgcccc tgaccaaagc gcgctaccag cggccccagc
    14401 gcccagatag aagcacgcat ggttttaacc agatcgtaag gtgcgcagaa tacattaacg
    14461 tcgcgggcat caatatgcac agaaccatta cgttctactt tcgcacccag ctggcttagc
    14521 agcttcattg atgtatcgac gtcttttagt ttcgggacgt tctggatctc taccggttct
    14581 tccgccagta gtgcggcaaa aaggataggc agagcagcat ttttagcgcc ggaaattgtg
    14641 acttcgccct ggagcttcgt tggcccctga acacgaaatt tatccattta gtttgttctc
    14701 agttaacaat tcatatccgc taccggcgaa tcgcccatag ctcaaaagcc gttcagtttg
    14761 cgatcgcgcg cccactccgc aggggtatac gctttgatcg acacagcatg aatgcggtta
    14821 tccgcaatat attccatcag cgggccatag accgtctgct gttttttaac ccgactcatg
    14881 ccgtcaaaca actcacccac ggcaataacc tgaaagtggc tgccatcgcc ggaaacgtgg
    14941 acttcctgga gggagagagc gttcatcaac acgctctgaa tttcattatt ttccatggga
    15001 tcttcaatca tcagttaata aatcagcgaa acatcttaga gcaaagttgc gctggcataa
    15061 ataagcaaaa agcctcgctg ataaatcaga caaggctcga cttgcaggca ggtttgccgg
    15121 acaggcggtt aacgccatat ccggcctgaa aaaatttaac gaggcagaac atcagcaggc
    15181 aaattataca atttcgccag ggtatacact ttgtcgttta ccccctgaag cgtcacattg
    15241 ttgccctgct ttttcgccag atcgataaga tggagcagca gtgccagtcc acccgtatcc
    15301 acgcgagaca cacggctaag atcgatgctg gtaatcccct tcaccgcttc ctcacgtatt
    15361 tcccaaagcg gtagcaaaac gtcctgatcc agctctccgg ataacgccag cgtgtcaccc
    15421 gtctgcatcc agctcagtga ctcgctcatt attttttctc ttccagagtg attttctgtt
    15481 gagaaatcga tttcagttgc gcagtcaggc cgtcgatacc tttggtacgc agcagcgttc
    15541 cccactcgtt ttgtttggtg gtgatcatac tgacgccttc agcaatcatg tcgtaagcct
    15601 gccagttgcc cgtctgggag tttttacgcc actggaagtc cagacgcacc ggcgggcggc
    15661 cattcgggtc aataatggta acgcgaatcg gcacaatggt tttatcaccc agcggctgtt
    15721 ctggcgcaat ctgataggtt tgaccgtgat acatcgccag cgcctgaccg taagcctgct
    15781 tcaggtactc acggaaagcg gcaaagtaag catcacgttg agctggggtc gcactcttgt
    15841 aatactggcc cagcaccagc gcaccggcgt atttcacctg tacgtatggc aacagttcct
    15901 gatcaacaat ggtacgcaga taatccgggt tggcccgaat ttgcggttgc tcattcttca
    15961 ggcgatcgaa cgttttctgc gccgcctcgt ccatcagctt atacggattg gtctggtctg
    16021 ccgcggttgc cgcactcagc ggtgcaatca ccagcaaagc gaccatcatt aaacgtttaa
    16081 acatgcgtcg gttctcctga aattatttcg ttgtacccac aggttcagtg gtttcattat
    16141 tacctggcgc agcagctggc gcatcgccac tattcttatt gtcatcgcct ttactaccgt
    16201 aaaggaactg accaatgaga tcttccagca ccatcgcaga cttggtgtcc tgaattgtat
    16261 cgccatcctt caggatagca gtccccagtt ccgggtcttc aaaaccgacg tttaatgcca
    16321 gatattgttc ccccagcagg ccggaagtac gaatgctcag cgaactggta tcaggaatgt
    16381 ggttataacg ttgttcaatt tccagcgtta cgcgcggcag ataggttttc gggtccagcg
    16441 taatatccgc cacccggccc acaacaacgc caccaatgct gaccggagag cgggctttca
    16501 ggccgccaat gttatcgaac gtcgcataaa gcgtataggt cggttcagta cgtatagacg
    16561 tcacgttcgc tgccttcagg caaacaaaca gcgccgcaag cagtgctgct aataaaaaga
    16621 tacccaccca aatttcattt tttttcgttt gcatgaactc aattcccaaa catcaatgcg
    16681 gtcagcacaa aatccagccc cagaacagcc agagacgagt ggacaacggt gcgagtggtt
    16741 gcccggctaa tcccggcaga cgtcgggatg gcgtcgtagc cgttaaacaa cgaaatccac
    16801 gtcaccgtga tggcgaacac cacgctctta atcagacagt tgacgagatc catacgccag
    16861 tcgacggcat tttgcattgc cgaccagaag aacccactat caatgccttt ccaactgacg
    16921 ccgaccagcg atccgcccca gatccccacg gcgacgaaga taaccgtcaa cagtggtaat
    16981 gaaataactc cagcccagaa acggggagaa ataacccgac gcagcggatc caccgccatc
    17041 atctccatac tggagagttg ctctgtagcg cgcatcaggc cgatttctgc ggttagcgcc
    17101 gaaccagcac gcccggcaaa caacaacgcg gcaaccaccg gccccagttc acgcagtagc
    17161 gataacgcca ccagcatacc cagactggtt tccgcactat aagtggtcag aaccagataa
    17221 ccttgcagcc ccaacaccat tccgatgaac acgccagaaa caacaataat cagcatcgac
    17281 aggacgccga cattatagag ctggcgcacc agcagcggtg catgtttgcg aaattccggt
    17341 ttgccgacca gcgcattgaa taacattaac ccggcccgcc cgaacgttct cagggtttta
    17401 atccctttat gtccgagcga cgccagcgca tttaacagca tgagtggctt aactccctgg
    17461 taaaagatca gcgtgataat cgccggcagg atagcggaac ggaacaggcc cgtcagctat
    17521 cccgtccaga aactgacgta cgcgcggatc aggattcgcc tgcaacgcct gggcactacc
    17581 atgagcgaca atttttttgt ccgccaggat ccaggcgtga tccgcaatac ttaacacttc
    17641 cggcacatcg tgagaaacca ccacacaagt cacgcccagc gcgctgttca gctcagaaat
    17701 cagcttcacc agcacgccca tggtaatggg atcttgccca acaaagggct catcaaacat
    17761 gatgagatcc ggctccagcg caatcgcacg tgccagcgct gcacgccgcg ccatcccacc
    17821 ggaaagttca gaaggcataa gtttagccgc tccacgcagc cccacggcct ccagcttcat
    17881 catcaccgta ctatgcaaca atggcgcggg aagttgggta tgttcccgca gtggataggc
    17941 gacgttgtca aatacgttca tatcagtgaa caacgccccg gactgaaata acatgctcat
    18001 ccgtttgcgc actgtataca ggcgcgaacg agacatcgcc ggaatattct caccatcgaa
    18061 aagaatctca ccatgatctg gtgcgatttg cccgccaatc agacggagta gcgtcgtttt
    18121 accgatgccc gatggcccca tgatcgccgt gatcttccct cgcggcacgg tcagggaaat
    18181 attatcgaag atgcagcgat tgccacgcgt aaaactgaca tcgcgcatat cgactaaatt
    18241 cgccacagac tgctccataa ttcacccttc gtcttgcgtt gattttctaa gcatggcgct
    18301 caatttaacc ttgaacccaa catatttaca gaatattacc tgccgtggtt agcgaaagct
    18361 ggcatttgtt ttacttttta gccgcataaa gtcaaaatta agaatctgtt acggctttct
    18421 gaaaatcttc agcggaccgg cgagtatacc tgaagaaagg acgttagatg cttttagcta
    18481 cggcactgtt aattgttggt ttacttttgg tcgtttacag tgccgaccgc ctggtttttg
    18541 ccgcgtctat tctttgccga acctttggca tcccgccgtt gatcatcggc atgacggtgg
    18601 tcagtattgg tacatcgtta ccagaaatca tcgtctcgct tgccgcgtct ttgcacgaac
    18661 aacgcgattt agccgttggt acagccctcg gctcaaacat tatcaatata ttgctgatcc
    18721 tcggtctggc tgcgctggtt cgtcctttta ccgtccattc tgatgttcta cgccgtgaat
    18781 tacccttaat gttgttggtc agcgtggtgg ccggttccgt actctatgac ggacaactta
    18841 gtcgcagcga tggtatcttt ctcctctttc tggctgtgct atggctgctg ttcattgtta
    18901 aacttgcacg tcaggctgaa cgtcagggga ctgacagcct gaccagagag cagcttgcag
    18961 agctgccgcg tgaaggtgga ttgcccgtcg catttttatg gctcggcatc gcactgatca
    19021 ttatgccagt ggccacgcgg atggtagttg ataacgccac ggtgctggcg aattactttg
    19081 ccatcagcga gttgacgatg ggtctgacgg caattgctat cggaaccagc ctgccggaac
    19141 tggcaaccgc aatagcaggg gttcgcaaag gtgaaaacga cattgcggtc ggaaatatca
    19201 ttggcgcaaa catttttaat attgtcatcg tgttgggttt acccgcgctg ataacgccag
    19261 gagagattga tccactggcg tacagtcgtg actacagcgt gatgttgctg gtgagcatta
    19321 tttttgcgtt gctgtgctgg cggcgctccc cgcaaccggg ccgtggtgta ggggtattat
    19381 taactggcgg atttatcgta tggctggcga tgttgtactg gttatcgcca atactcgttg
    19441 aataactgga aacgcattat gtcgcacgta gagttacaac cgggttttga ctttcagcaa
    19501 gcaggtaaag aagtcctggc gattgaacgt gaatgcctgg cggagcttga tcaatacatc
    19561 aatcagaatt tcacgcttgc ctgtgaaaag atgttctggt gtaaagggaa agttgtcgtc
    19621 atggggatgg ggaaatcggg gcacatcggg cgcaaaatgg cagcaacatt tgccagcact
    19681 ggtacacctt catttttcgt ccatcctggt gaagccgcgc atggtgattt aggcatggtc
    19741 accccgcagg atgtggtgat tgctatctct aactcaggtg aatccagcga aatcacggcc
    19801 ttaattccag tgcttaagcg tcttcacgta ccgttaatct gcatcaccgg tcgcccggag
    19861 agcagcatgg cgcgcgccgc agatgtgcat ctgtgtgtta aagtagcgaa agaagcctgt
    19921 ccgttagggc tggcaccgac cagcagcaca accgccacgc tggttatggg cgatgccctc
    19981 gctgtcgcgc tgttaaaagc acgcggcttt actgctgaag attttgcgct ctcacaccca
    20041 ggcggcgcac tgggtcgtaa acttctgctg cgcgtaaacg atattatgca tacgggcgat
    20101 gagatcccgc atgttaagaa aacggccagt ctgcgtgacg cattgctgga agttacccgc
    20161 aaaaatcttg gtatgactgt catttgcgat gacaatatga tgattgaagg catctttacc
    20221 gacggtgatt tacgccgtgt cttcgatatg ggcgtggatg ttcgtcagtt aagtattgcc
    20281 gatgtgatga cgccgggggg aatacgcgtg cgcccaggca ttctggccgt tgaggcactg
    20341 aacttaatgc agtcccgcca tatcacctcc gtgatggttg ccgatggcga ccatttactc
    20401 ggtgtgttac atatgcatga tttactgcgt gcaggcgtag tgtaaagatt caaggataaa
    20461 caacaatgag caaagcaggt gcgtcgcttg cgacctgtta cggccctgtc agcgccgacg
    20521 ttatggcaaa agcagagaac attcgtctgc tgatcctcga cgttgacggc gtactgtcag
    20581 atggcctgat ttatatgggc aataacggcg aagagctgaa agcgttcaat gttcgtgacg
    20641 gttatggcat tcgttgtgcg ctcacctctg atattgaagt cgctatcatt accgggcgaa
    20701 aggctaaact ggtagaagat cgttgtgcca cattggggat cactcacttg tatcaggggc
    20761 agtcaaacaa actgatcgcc tttagcgatc tgctggaaaa actggcgatt gccccggaaa
    20821 atgtggctta tgtcggcgat gatctcatcg actggccggt aatggaaaaa gtgggtttaa
    20881 gcgtcgccgt ggccgatgcg catccactgt tgatcccgcg cgccgattac gtaacgcgca
    20941 ttgctggcgg tcgtggcgca gtgcgagaag tttgcgactt attactcctg gcgcagggca
    21001 aactggatga agccaaaggg caatcgatat gagtaaagcc agacgttggg ttatcattgt
    21061 gctatcactg gcggttctgg tgatgatcgg cattaatatg gccgaaaaag acgataccgc
    21121 ccaggtggtc gtcaacaaca atgatcccac ctataaaagc gagcatacgg acacgctcgt
    21181 ctataaccca gaaggggcac taagctatcg attgattgct caacacgttg aatattattc
    21241 cgatcaggcc gtttcgtggt ttacgcagcc ggtacttacc acgtttgata aggataaaat
    21301 cccgacatgg tccgtaaaag cagataaagc caagctgacc aatgaccgga tgctctattt
    21361 atatggacac gttgaagtca atgcactcgt gccggactct caacttcgca gaatcacgac
    21421 ggataacgcg cagatcaatc tggtgacgca ggatgttacc tctgaagacc tcgtcacgtt
    21481 atacggaaca acatttaact ccagcggtct gaaaatgcgc ggcaacttac gcagcaagaa
    21541 cgccgagctg attgaaaagg ttagaacatc ctatgaaatt caaaacaaac aaactcagcc
    21601 ttaatcttgt gcttgccagc tcacttctgg ccgccagcat tccggcattt gccgtaaccg
    21661 gagacactga tcagccgatc cacattgaat cggaccagca atctcttgat atgcaaggca
    21721 acgtggttac ctttaccggt aatgttatcg tcacccaggg caccatcaaa attaatgccg
    21781 acaaagtggt cgttacccgt ccgggcggcg aacaaggtaa agaagtgatt gacggctacg
    21841 gtaaaccggc aacgttctac cagatgcagg acaacggtaa acccgttgaa ggtcacgctt
    21901 cccagatgca ctacgaactg gcaaaagatt ttgtcgttct gacgggtaat gcttatctgc
    21961 agcaggtcga tagcaacatt aagggcgata agatcactta cctggtgaaa gagcagaaaa
    22021 tgcaggcttt cagcgacaaa ggcaagcgcg taaccaccgt tctggtgccg tcgcagttgc
    22081 aggacaaaaa caataaaggc cagaccccgg cacagaagaa gggtaattaa ttcgttatgg
    22141 caacattaac tgcaaagaac cttgcaaaag cctataaagg ccgtcgcgtg gtagaagacg
    22201 tcagcctgac cgtcaactcc ggggaaattg tcggtctgct ggggccaaac ggtgccggta
    22261 agaccaccac tttctacatg gttgttggca ttgtgccgcg cgatgcgggc aacatcatta
    22321 ttgatgatga cgatatcagt ctgctgcctc tgcatgcacg cgcgcgccgc ggtatcggct
    22381 atctgccgca ggaagcctcc attttccgtc gcctcagcgt ttacgataac ctgatggcgg
    22441 tactgcaaat tcgtgacgac ttgtctgctg aacaacgtga agaccgtgcg aacgagctga
    22501 tggaagagtt tcacattgag cacctgcgtg acagcatggg gcagtcactc tccgggggtg
    22561 aacgtcgccg tgtagaaatt gcccgcgcac tggctgcgaa tccgaaattt attctgctcg
    22621 acgaaccgtt tgccggggtt gacccgatct cggttatcga cattaaacgc atcattgagc
    22681 acctgcgcga cagcggcctg ggcgtactga tcactgacca caacgtgcgt gaaacactgg
    22741 cggtttgtga acgcgcttat atcgtcagtc aggggcattt gatcgcccac ggcacgccta
    22801 cagaaatctt acaagacgaa cacgttaagc gtgtatacct tggggaagac ttcagactct
    22861 gatagggtag aagtttgcga cgttttagca ggagagtacg attctgaaca tgaagcaagg
    22921 tttgcaactc aggcttagcc aacaactggc gatgacgcca cagctccaac aggcaattcg
    22981 tctgttgcag ttgtcgacgc tggaacttca gcaagagcta cagcaggcgc tggagagcaa
    23041 tccgctgctt gagcaaatcg acactcatga agaaatcgac acccgcgaaa cgcaagacag
    23101 tgaaacactg gacaccgccg acgcgctcga acaaaaagag atgccggaag agctgccgct
    23161 cgatgccagt tgggacacca tttacaccgc tggtacacca tccggcacca gcggtgacta
    23221 cattgatgac gagctgccgg tctatcaggg cgaaacgacg cagaccttac aggattacct
    23281 gatgtggcag gtcgagttga caccgttttc cgacactgac cgcgcgattg ctacctctat
    23341 cgtcgatgcc gttgatgaca ccggttatct gactgtcccg ctggaagata ttctcgaaag
    23401 tatgggcgat gaagagattg acatcgacga agttgaagcc gtccttaagc ggatccaacg
    23461 gtttgatcca gtcggtgtgg cggcaaaaga tctgcgtgac tgcctgctga tccaactctc
    23521 ccaattcgat aagaccacgc catggctgga agaggccaga ctgatcatta gcgatcatct
    23581 cgatctatta gccaatcacg acttccgcac tttaatgcgc gtcacgcgtc tgaaagaaga
    23641 tgtgctgaaa gaagccgtca atctgatcca gtcgctcgat ccgcgccccg ggcagtcgat
    23701 ccagactggc gaacctgagt atgtcattcc agatgtgctg gtgcgtaagc ataacggcca
    23761 ctggacggta gaactcaaca gtgacagcat tccgcgtctg caaatcaacc agcactacgc
    23821 ctcgatgtgc aataacgcgc gcaatgatgg tgatagccag tttatccgca gcaatctgca
    23881 ggatgccaaa tggttgatca agagtctgga aagccgtaac gatacgctac tgcgcgtgag
    23941 tcgctgtatc gttgaacagc agcaagcctt ctttgagcaa ggtgaagaat atatgaaacc
    24001 gatggtactg gccgatatcg cccaggctgt cgaaatgcat gaatcgacga tatctcgcgt
    24061 gaccacgcaa aaatacctgc atagtccacg aggcattttt gaactgaaat atttcttttc
    24121 cagtcacgtc aatactgaag gcgggggtga agcctcttcc acagcgattc gtgcgctggt
    24181 gaagaaatta atcgcggcgg aaaacccagc gaaaccgttg agcgacagca agttaacctc
    24241 tttgctgtcg gaacaaggta tcatggtggc acgccgcact gttgcgaagt accgagagtc
    24301 tttatccatt ccgccgtcaa accagcgtaa acagctcgtt tgacccaacc gataaggaag
    24361 acactatgca gctcaacatt accggaaata acgtcgagat caccgaggca ctgcgcgaat
    24421 ttgttacagc caaatttgcc aaacttgagc aatattttga ccgaatcaac caggtctatg
    24481 ttgttctgaa agtggagaaa gtcacccaca cctcagatgc aacactgcat gtaaacggtg
    24541 gtgaaattca tgccagcgcg gaaggtcagg acatgtacgc tgccattgat ggcttaattg
    24601 ataagctggc acgccagttg accaaacata aagataaact gaaacaacac taattgtccg
    24661 ggcaattagc atgtgcatgg cggcctgttg tgcggcacaa cgggccattt gtacggttaa
    24721 tgctccgaac ctgttccact gtttgagtgg gcaggttctt aggtgaaatt atgacaaata
    24781 atgatacaac tctacagctt agcagtgttc ttaacaggga atgtacgcga agccgcgtcc
    24841 actgccagag caaaaaacgc gccctggaaa tcatcagcga gctggcggcg aaacaactta
    24901 gcctggcgcc tcaggtggtt tttgaagcta tcctgacgcg ggaaaaaatg ggcagtaccg
    24961 gtatcggcaa tggtattgcc attccgcatg gcaaactgga agaagatact ctgcgtgccg
    25021 ttggcgtttt cgttcagctc gaaacaccta tagctttcga tgccatcgac aaccaaccgg
    25081 tggacttact ttttgccctg ctggtgccgg cagaccaaac taaaacgcac ctgcatactc
    25141 tgtcgctggt ggcgaaacgt ctggcggaca aaaccatctg ccgccgttta cgcgcagccc
    25201 agagcgatga agagctgtat caaatcatta cggataccga aggtactccg gatgaagcgt
    25261 agttattcgg taatgtctct tttagacgtt gtgaggagaa acagtacatg gtactgatga
    25321 tcgtcagcgg acgttcaggt tcaggtaaat ctgtcgccct gcgtgcgctg gaagatatgg
    25381 gtttttactg cgtggataac cttcccgtag tgttgttacc cgatctggct cgaactctgg
    25441 ccgatcgaga gatttctgcc gccgtcagca ttgatgttcg taatatgccg gagtcaccag
    25501 aaatattcga acaggcgatg agtaacctgc ctgacgcttt ctcaccgcaa ctactgttcc
    25561 tggatgccga ccgtaatacc ttaattcgtc gttacagtga cacgcgccga ctgcatccgc
    25621 tttccagcaa aaacctgtcg ctggaaagtg ctatcgacaa agaaagcgat ttgctggagc
    25681 ctctgcgttc gcgagcggat ctgattgtcg atacctcaga aatgtccgtt cacgagctgg
    25741 cagaaatgct gcgtacccgt ctgttgggta aacgtgaacg tgaactgacc atggtctttg
    25801 agtctttcgg cttcaaacac ggtatcccta tcgatgcaga ttacgtcttt gacgtgcgct
    25861 tcttgccgaa cccacactgg gatccgaaac tgcgtccaat gacaggtctt gataaacctg
    25921 tcgccgcgtt cctcgaccgc cacacagaag tacacaattt tatctaccag acgcgaagct
    25981 atcttgagct atggttacct atgctggaaa ccaacaaccg tagctacctg acggtcgcca
    26041 ttggttgtac cggcgggaag caccgttcgg tgtatattgc agagcaactg gcagactact
    26101 tccgctcgcg cggtaaaaac gtccagtcac gccatcggac gctggaaaaa cgtaaaccat
    26161 gaccgtcaag caaactgttg aaattacaaa caagctgggc atgcatgccc ggcctgcaat
    26221 gaagctgttt gaattaatgc agggttttga cgctgaagtg ctgttacgta atgacgaagg
    26281 caccgaggct gaagccaaca gcgttattgc gctgctgatg ttggattctg ccaaaggacg
    26341 gcagattgaa gttgaagcga ccggtccaca ggaagaggaa gcactggccg ccgttatcgc
    26401 cctctttaac tctggttttg atgaagatta atcttcatca ctttgacatt caaatccctt
    26461 caagctcccc cggcaataag aaaagcgcgt actgaaatcg ttatcaatca acgtcgattg
    26521 tacatttcgt gcgcttttta ctttcagaag aaccttaaga aaaccttaag aagcattgtt
    26581 tagatttcgt ttaagttaat cgaccatact ggagatcgtc agaaaatatt tccaggagat
    26641 ggcatgattc gcttatctga acaaagtccc ctgggcaccg gaagacatcg gaaatgttat
    26701 gcgcatccgg aagatgccca acgctgtatc aagattgtct accatcgtgg cgatggtggc
    26761 gataaagaga tccgccgtga gttaaagtac tacgcgcatc ttggtcgccg gttaaaagac
    26821 tggagtggaa taccgcgcta tcacggtacc gttgagacgg attgcggaac cggttatgtc
    26881 tacgatgtga ttgccgattt cgacggcaag ccttccatta cgctgaccga atttgcagaa
    26941 caatgccgtt acgaagaaga cattgcacaa ctgcgccagt tactgaaaca gctgaagcgt
    27001 tatttgcagg ataaccgtat cgtgacgatg tcgctgaagc cgcagaatat cctctgtcac
    27061 cgcatcagtg agtcagaggt tatccccgtc gtctgcgaca acattggtga aagcacattg
    27121 attccactgg cgacctggtc gaagtggtgt tgtctgcgta agcaagagcg gttatggaaa
    27181 cgatttatcg cccaaccggc gctggctatt gccctgcaga aagatctgca acctcgtgag
    27241 agcaaaacgc tggccctcac ttcgcgcgaa gcttaatcaa gctggtgttg ttgcataaac
    27301 ggctcaccgc ctaactgata catctgccgt aaaatccacg cctgacggct acgcacatag
    27361 cccgatggcg cggagacttt gaaacgaagt ggattaggta atacagctgc cagtaatgca
    27421 gcttccgacc gggtaagttt gctcgcgggt ttgtggaaat aacgttgtgc cgcagcttcg
    27481 acgccaaaca caccgtcgcc aaattcggcg atattcaggt aaaccgtcag gatacgcttt
    27541 ttgctccaga ctgtttctat ccccagcgtt aatccggctt ccagcccttt tcgaacccag
    27601 ctacgcccat cccataaaaa gagatttttg gctgtctgct gagaaatcgt tgaagcaccg
    27661 cgaatacggt tttcattgcg ctcgttgtgc gccagggctt gctcaatgga agcgacatca
    27721 aagccccagt gctcaggaaa tttctgatct tctgcggcaa tcaccgccag tcccatccac
    27781 ggcgagattt gatccatact gacccagtca gaatgtgcca cgtaacgaaa attgccatgc
    27841 agccaggcgc tgacttgccg ctcgaccatt accgctgaga aaggaacagg cgcaacgcta
    27901 aacaacgcga tgcccccgcc ccagaaaatg gcgaggacaa ccattaaccg cagaagaaaa
    27961 cggcgaacga aactaaacac cgttaagcgg cttttactca ttcagccaga accagcacgc
    28021 gggaaaccag cttatcaatg ccgctcgccg cttctgcaat gttctgcgcc agcatatatg
    28081 ccggggtggt gacaatctta ttgtcttcat caaccacgat atcatcgaca ggacacggca
    28141 catgctccgc gcccatctct tccagcactt ctgcggtatc gatatcagta ccgatggtca
    28201 aacgtagcgg gaaatcgaaa attttcggca gtatcgccgg tgcaatacac ataaaaccaa
    28261 gcggttttcc ggcctgatgc atcgcttgtg ccagcgcctt taattcacgg tcaacggtgc
    28321 attcgctacc aagactggca aaattgctta aattcttcgc cgcgccaaaa ccccccggca
    28381 caatcaacgc atccagttca gcggcatcgg cctgggccag aggacggatt tcaccgcgcg
    28441 ttattcgtgc cgcttcaatc agcacattgc gcgtttccgt catcgcttca ccagttaaat
    28501 ggttgataac atcaacctgc tgcttatccg gtgcaaagca aaccgcctgc gcaccgctgc
    28561 gtgaaatagc taacaacgtc aataccgctt catgaatttc agaaccgtca tagacgccgc
    28621 atccgctcag aattacgcca attttcttca ttgtgatctt cctttcgcaa ttgactgaaa
    28681 cacattaatt tttttaataa aaatgctacg catcacacat ttaactgatt catgtaacaa
    28741 atcatttaag ttttgctatc ttaactgcgt gcggcctgaa aaacagtgct gtgcccttgt
    28801 aactcatcat aataatttac ggcgcagcca agatttccct ggtgttggcg cagtattcgc
    28861 gcaccccggt ctagccgggg tcatttttta gtggcttttg ccacccacgc tttcagtact
    28921 tctacgtcgt gacgccactc ttctttcatc tcttcaatcc attcaccgac gttatcttcc
    28981 caggcgggaa ggtcaggaga ctgaatttgc tgacccagct gttgcagatg gcgtaacccc
    29041 actgaccccg ccgcaccttt aattttatgc ccttcctcaa caatgccttt tttatcctgc
    29101 gccgtcagat tcgactccag cacgctgaca tagcccggca tcattctctc aaacaccgct
    29161 aacccgtcgg tgatcagctt cggtcctaca agttcgagat actgttccag catgggaata
    29221 tcgagcaatg cttctgattt actgttctct tctgtcgtca ccgtactctc ctcatcatcc
    29281 tgggtatccc agaatttctt gatcatcgcg gttagcgccg gaacagaaag cggcttactc
    29341 agcacatcat ccattccagc attgaggtac tcttgcttgt ctttcagcac gttagcggtt
    29401 aaggccacca gcggcggtaa atcctcgcgc ggataacgct tcgtcagttc acgagagata
    29461 tccagcccgg tcatatctgg caactgaata tccagcaata ccaggtcgta ttcgcccggt
    29521 ttaaacatct ccagcgccgc cttgccggtc atggcgacat caacgctgtt acctaatttt
    29581 tccagcacag aacgcgcgac aatcacgttc agttcaatgt cttccaccag cagcacattc
    29641 agcgccggta aaggcatatc gtcttcatca aacgcatcat caacctcttc tgctaccgac
    29701 ggtgcgtgga tcgtcaacgt aaaggttgaa cctttgccct gttcgctggt aaccgtaata
    29761 tcgccgccca tatttttcgc cagacgacga gaaacggcca gaccaatacc ggtgccggtg
    29821 gcaggtttac cgccatgact gtctttcacc tggtaataca tggcgaagat tttatccagc
    29881 tcatcctgcg gaatgccgat accagagtct tccacttcaa aatgcagcat atcgccttca
    29941 tcgtagcgca cgcgcacggt aacctggcct tgctgagtga atttgacggc gttactgatg
    30001 aggttccaca agatctgccg taaacgcgtc ccgtcggtaa tgacctgatg cggtaatggc
    30061 agcgtcggct ccagattaaa gcgcaatcct ttttgttgcg cctgcaatgc ggagagattt
    30121 tccagatcgg caaggaagct ggtgaaatca accggctgat tatcaagctg gaccttgcgc
    30181 cgttccatct tatccatgtc gataatatcg ttgaagatat tccccagcgt gacagccgaa
    30241 acatggatgg ttttgagata tttttcctgc tcggcggtga gttcagtatc cagcagaatg
    30301 cggctcaggc cgacgatacc gttcagcggt gtacgcaatt cgtgactgat ggtggagata
    30361 aacgtcgttt tgtcgcggct ggcgcgttca agcgcatcct gataccgctt acgctcggta
    30421 atgtcgcgac caaagcccat caaaccgtga cgtttaccca cgcggtcgta gtacggcact
    30481 ttacggattt caaagcaggc tttgcgtccg tcgggataat ccagccactg ctcataggtc
    30541 agtgacacat tatgacggaa cactttttca tcggtttcaa tgactttcgc agcggcttct
    30601 ggtgagtaaa catcagcagg tttcaggtga accagttgtt tttcgctttt tccggtcagc
    30661 agctccatcg cgcggttaca gccggaaaac tctttatctt cgttacgata aaaaaccagg
    30721 tcgggtgaag cgtcaaggaa ggaacgtaag aatgaggatt gctgctcgag ctgaatttgt
    30781 gtctcttcgc gctctttgat ttcaattttc agttggccga aggtttcctg cagttctgct
    30841 tccgcttttt cacgaacggc gatttcctga tttagctggg caatattatc ttttaactga
    30901 acgttgaggc tcaaatcgcg ctcgcgcatc tcctccagtt tttgcaccag tcgtgacaga
    30961 cgttgtcgtg actcctccag ttgctcgacg accaccgata gaaagtagac cgcccacggc
    31021 gtaatcagca aaccaaagaa gatagaacga ataacatcaa tgctttcgac ctgaccatgc
    31081 agcaccatgg ttaccgccat ttgtaccaca atggcaagaa cgacgagggc cagcgccagc
    31141 aacattgaga agcgcaccag acctaacttc atcatcaggt caacataata ctgcgccagc
    31201 agacgaattt gcttcattag ggaattcctt cacgacaacc tgccccaata atacccaatt
    31261 ctgacagtta cgttataaat tgtgtgagaa atgcggaatt cccctcaatg gcctacgccg
    31321 ttggaggtag ccacggacgt cccagcgccg atccctgtac gccatgttca ttcagataac
    31381 gatccagctc gaccattccc gtccatcgat tttcgcacca cagcggagca agcagcgtcg
    31441 ggcgacgggc gctggcggaa atacggtggt agataacttc cggtggcgta tggcgaatca
    31501 tttctccggc agtgagcgtg taatcctcca gttcaattcc gttcaaacgc cccgcttccc
    31561 aggctttcgc cataatgctg cctttcacta tatgcagcgg atgcagcttt atgccgtcca
    31621 cgccggtttc aaccactcgc tccagcgttt gcaaacattc agcctgccct tcgccaggca
    31681 ggccaacaat taagtgagag cacaccttca gcccacgctc acgcgccagc tgcgtcgtgc
    31741 gctgatagca ggcaaaatca tgaccgcgat ttatgcgatg cagtgttttg tcgtgggcgg
    31801 tttgtagccc cagctccagc cacacttcgt agccctggtc cttatattcg caaagcagat
    31861 ccagcaccgc atccggcacg cagtccgggc gggtaccaac acacaaaccg acaatattgg
    31921 cctggctcac cgcctgctga tacatcgaac gcagcacctg aacttccgca aaggtgctgg
    31981 tatacgcctg aaagtaggcc agatagcgtt tagcgcggtt aactaaattc gcctggtgcg
    32041 ccagttgctc ggcaatggaa cgatgctgct gcgcttcatc ggcaaacgag gcaacattac
    32101 agaatgtgca gccgccacgc ccgatggtac cgtcacggtt agggcagcta aaaccgccgt
    32161 gcagcgtcag cttatgcacc ttttgcccat aacgacgggt gagatcacca ccaaacatat
    32221 tgactaattt ctgtaactgc ataatctgat agaccgcgcc ttgaaaagag gccaaagcct
    32281 gccattttta gcccaattcg gcgatgacct ggatcaatcg tcccagcctg cttttatcaa
    32341 ctgcataacc aatcaaaatt accgaaattt catgcacaat cacataaatc actgttgctt
    32401 atcttgtgtc agattttttt atcttcttat ggattttagg caaaaacagt agcatgaaac
    32461 gttattacca attaaggcag tataaaatgc tggctttctc atcaattcaa ggcaggataa
    32521 gggttaacac gcctttatga cagtcaggaa ttggctgttt ctctaacgac acccctttta
    32581 gccttaaata gaaaatccac tttaatttca tacaattaat aaagaatttt gcgctaaagc
    32641 acatttctgt accaataagc ttgccatttg acctgtatca gctttcccga taagttggaa
    32701 atccgctgga agctttctgg atgagcagcc tgctcatcat atttatgcag taattgagat
    32761 cccctcttca ccgtattaac cgatgcgaaa aggacaacaa gggggcgaat gcgaggtgcg
    32821 cgtatgacac gcaaaccccg tcgccacgct cttactgtgc ccgtgcgcag cggttcggaa
    32881 gtggggttcc cgcagagcct gggggaggtt cacgatatgt tgtacgataa atcccttgag
    32941 agggataact gtggtttcgg cctgatcgcc cacatagaag gcgaacctag ccacaaggta
    33001 gtgcgtactg caatacacgc actggcccgc atgcagcacc gtggcgcgat tctcgccgat
    33061 ggtaaaaccg gtgacggttg cggcttgctg ttacaaaaac cggatcgttt ttttcgcatc
    33121 gttgcgcagg agcgcggctg gcgtttagca aaaaactacg ccgtcgggat gctcttcctg
    33181 aataaagatc ctgaactcgc cgctgccgca cgccgcatcg ttgaagaaga gctgcaacgc
    33241 gaaacgttgt cgattgtggg ctggcgtgat gtccccacta acgaaggcgt gctgggtgaa
    33301 atcgccctct cctctctgcc acgcattgag caaatttttg tgaacgcccc ggcaggctgg
    33361 cgtccgcgcg atatggagcg ccgtctgttt atcgcccgcc gccgcattga aaagcgtctc
    33421 gaagccgaca aagacttcta cgtctgtagc ctgtcgaatc tggtcaatat ttataaaggt
    33481 ctgtgtatgc cggcggatct gccgcgcttc tacctggacc tcgcggacct gcgtctggaa
    33541 tcggccatct gcctgtttca ccagcgcttt tccactaaca ccgtaccgcg ctggccgctg
    33601 gcgcagccgt tccgctacct cgcgcataac ggtgaaatca acaccatcac tggtaaccgc
    33661 caatgggcgc gtgcgcgtac ctataaattc cagacgccgc tgatccctga cctgcacgac
    33721 gccgcgccgt tcgtcaacga aaccggctct gactccagtt cgatggataa catgctggaa
    33781 ctcctgctgg caggtgggat ggatatcatc cgtgccatgc gtctgttagt gccacctgcc
    33841 tggcagaaca acccggatat ggacccggaa ctgcgtgcct tctttgactt taactccatg
    33901 catatggagc cgtgggatgg cccggcaggc atcgtgatgt ctgacggtcg ttttgccgcc
    33961 tgtaacctcg accgtaacgg tctgcgtccg gcgcgctacg tcatcaccaa agataagctc
    34021 attacctgcg cctctgaagt cggtatctgg gattatcagc ctgacgaagt ggtcgaaaaa
    34081 ggccgcgtgg ggccaggcga actgatggtt atcgacaccc gcagtgggcg tattctgcac
    34141 tcggcagaaa ccgatgacga tctgaaaagc cgccaccctt ataaagagtg gatggagaaa
    34201 aacgtccgcc gtctggtacc gtttgaagat ctgcccgatg aagaagtggg tagccgcgaa
    34261 ctggacgacg acacgcttgc cagctaccag aaacaattta actacagcgc ggaagagctg
    34321 gattcggtga tccgcgtact gggtgaaaac ggtcaggaag cggtcggttc gatgggcgac
    34381 gataccccat tcgccgtgct ctccagccag ccgcgcatta tttacgacta cttccgccag
    34441 cagtttgctc aggtaaccaa cccgccaatc gacccgctgc gcgaagcgca tgttatgtcg
    34501 ctcgccacca gtatcggtcg tgaaatgaac gtcttctgtg aagcagaagg ccaggcgcac
    34561 cgtttaagct ttaaatcgcc gattctgctc tattccgatt tcaaacagct cacgacgatg
    34621 aaagaggatc actaccgcgc agatacgctg gatatcacct ttgacgtcac taaaaccacg
    34681 ctcgaagcga cggtgaaaga actgtgtgac aaagccgaaa aaatggtacg tagcggcacc
    34741 gtgctgctgg tgctctccga ccggaatatc gctaaagatc gcctgccggt tccggctccg
    34801 atggcggttg gcgcgatcca gacccgtctg gtcgagcaaa gcctgcgttg cgatgccaac
    34861 atcatcgtag aaaccgccag cgcccgtgat ccgcaccact tcgccgtgtt gctgggtttc
    34921 ggcgcgacgg ctatttatcc ataccttgcc tatgaaacgc tgggccgcct ggtagacacc
    34981 cacgcgattg ccaaagatta tcgtaccgtg atgctcaact atcgtaacgg catcaacaaa
    35041 ggcttgtaca aaatcatgtc caaaatgggc atctccacca tcgcctctta ccgctgctcg
    35101 aaactgtttg aagcggtcgg tctgcacgat gatgtcgtgg gcctgtgctt ccagggggca
    35161 gttagccgca ttgggggggc gagctttgaa gacttccagc aggatctgct gaatctgtcg
    35221 aaacgagcct ggctggcgcg taagcctatc agccagggcg gcctgctgaa atatgtacac
    35281 ggtggcgaat accacgccta caacccggac gtggtgcgca cgctgcaaca ggcggtacag
    35341 agcggcgagt atagcgacta tcaggaatac gcgaagctgg ttaatgagcg tccggcaacc
    35401 acgctacgcg atctgctggc aattacgccg ggtgaaaacg cggtcaacat tgctgatgtt
    35461 gaaccggcaa gcgaactgtt taaacgcttc gataccgccg cgatgtctat cggtgcgtta
    35521 agcccggaag cccacgaggc gctggcggaa gcgatgaaca gcatcggcgg taactcaaac
    35581 tccggcgaag gcggtgaaga cccggcgcgc tatggcacta acaaagtgtc gcgcatcaag
    35641 caggtagcgt ccggtcgctt tggggttact ccggcgtatc tggtcaatgc cgacgtcatt
    35701 cagataaaag tcgcccaggg cgcgaagccg ggtgaaggtg gtcagttacc gggtgataaa
    35761 gtcactcctt acattgccaa actgcgctat tcggtgccgg gcgtgacgct gatctccccg
    35821 ccgccgcacc acgatatcta ctctattgag gatttagcgc agctgatttt cgacctcaag
    35881 caggtcaatc caaaagcgat gatctccgtg aagctggttt ccgaaccggg cgtaggcacc
    35941 atcgcgactg gcgtggcaaa agcttatgcc gatttaatca ccatcgcagg ctatgacggc
    36001 ggcaccggcg caagtccgct ttcatcggtg aaatacgcag gctgtccgtg ggagctgggg
    36061 cttgttgaaa cccaacaggc gctggttgcc aacggcttgc gtcacaagat ccgtttgcag
    36121 gtcgatggcg gcctgaaaac gggtgtcgat atcatcaaag cggcgattct tggcgcggaa
    36181 agttttggct tcggcactgg cccgatggta gcgcttggct gtaaatatct gcgaatttgc
    36241 cacctgaaca actgcgccac gggtgtagca actcaggatg acaaactgcg taagaatcac
    36301 tatcacggcc tgccgttcaa agtgactaat tactttgagt ttatcgcccg tgaaacccgc
    36361 gagctgatgg cacagctggg ggtaacgcgt ctggtggatc tgattggtcg caccgacctg
    36421 ctgaaagagc tggacggttt caccgccaaa cagcagaaac tggcactgtc gaagctgctg
    36481 gagactgccg agccgcatcc tggtaaagcg ctctactgca ccgaaaacaa cccgccgttt
    36541 gataacggcc tgctgaacgc gcagttgctg caacaggcaa aaccgtttgt cgatgaacgc
    36601 cagagcaaaa ccttctggtt cgatatccgc aacaccgacc gttctgtcgg cgcgtcgctt
    36661 tcaggctata tcgcccagac gcacggcgat caggggctgg cagccgatcc tatcaaagcg
    36721 tacttcaacg gcactgcagg ccagagcttc ggcgtgtgga acgcgggcgg cgtggaactg
    36781 tacctgaccg gtgatgccaa cgactatgtt ggtaaaggca tggcgggcgg cttaatcgcc
    36841 attcgtcctc cggttggttc cgccttccgc agccatgaag cgagcattat cggcaacacc
    36901 tgcctgtatg gcgcgaccgg tggtcgtctg tatgccgcag gccgcgcggg tgaacgtttc
    36961 ggcgtgcgta actccggtgc tatcaccgtg gtagaaggca ttggcgacaa cggctgtgaa
    37021 tatatgacgg gtggtatcgt ctgtattctg ggtaaaaccg gcgttaactt cggcgcgggc
    37081 atgactggcg gcttcgctta cgttctcgat gaaagcggcg atttccgcaa acgcgttaac
    37141 ccggagctgg tcgaggtctt aagcgttgac gatctggcga tccatgaaga gcatctgcgt
    37201 ggtcttatca ccgagcatgt tcagcatacc ggttcccagc gtggtgaaga gattctggcg
    37261 aactggtcaa ccttcgccac taaatttgcg ctggttaaac cgaagtccag tgatgtaaaa
    37321 gcactgctgg gtcaccgtag tcgtagcgca gcagagttgc gcgtgcaggc gcagtaaggg
    37381 gtagcaacaa tgagtcagaa tgtttatcaa tttatcgacc tgcagcgcgt tgatccgcca
    37441 aagaaaccgc tgaagatccg caaaattgag tttgttgaaa tttacgagcc gttttccgaa
    37501 ggccaggcca aagcgcaggc tgaccgctgc ctgtcgtgcg gcaacccata ctgcgagtgg
    37561 aaatgcccgg tacacaacta catcccaaac tggctgaagc tcgccaatga ggggcgtatt
    37621 tttgaagcgg cggaactgtc gcaccagaca aacaccctgc cggaagtttg cggacgtgtc
    37681 tgcccgcaag accgtctgtg cgaaggttcc tgcaccctga acgatgagtt cggtgcggtg
    37741 accatcggca acattgagcg ttatatcaat gataaagcgt tcgagatggg ctggcgtccg
    37801 gatatgtccg gcgtgaaaca gaccggtaaa aaagtggcga ttatcggcgc aggtccggcg
    37861 ggtctggcgt gcgcggatgt cctgacgcgc aacggcgtaa aagcgatcgt cttcgaccgt
    37921 cacccagaaa ttggcggctt gctgaccttc ggtattccgg ctttcaagct ggaaaaagag
    37981 gtaatgacgc gccgccgtga aatcttcacc ggcatgggta ttgaattcaa actcaatacc
    38041 gaagtgggcc gcgacgtgca gctggacgat ctgctgagtg attacgatgc cgtgttcctt
    38101 ggcgtcggga cttatcagtc aatgcgcggc gggctggaaa acgaagacgc cgatggcgtg
    38161 tacgcagcgc tgccgttcct tatcgccaac accaaacagt taatgggctt tggtgaaacc
    38221 cgcgacgaac cgttcgtcag catggaaggc aaacgcgtgg tggtccttgg cggtggcgac
    38281 actgcgatgg actgcgtgcg cacgtctgta cgccaggggg caaagcacgt tacctgtgcc
    38341 tatcgtcgtg atgaagagaa catgccgggt tcccgccgcg aagtgaaaaa cgcgcgggaa
    38401 gaaggtgttg agttcaaatt caacgtccag ccgctgggta tagaagtgaa tggcaacggc
    38461 aaagtcagcg gcgtaaaaat ggtgcgcaca gaaatgggcg aaccggatgc caaaggccgt
    38521 cgccgcgcgg agattgtggc aggttccgaa catatcgttc cggcagatgc ggtgatcatg
    38581 gcgtttggtt tccgtccaca caacatggaa tggctggcaa aacacagcgt cgagctggat
    38641 tcgcaggggc gcatcatcgc cccggaaggc agcgacaacg ctttccagac cagcaacccg
    38701 aaaatctttg ctggcggcga tatcgtccgt ggttccgatc tggtcgttac cgccatcgcc
    38761 gaaggtcgca aagcggcaga cggcattatg aactggctgg aagtttaagc gaggtaacaa
    38821 tgaattcgcc agacagtgtc tggcgaattt ataaaacgag ctatccaact aaattttaag
    38881 ccattaaatc agccttaacc ttcactacca cctttttaat ctcaccaggt tcgccaacaa
    38941 cgcatcccgg tttatgcggt tctcctggca taaacacggc gaacattccc ggtttcagga
    39001 tgatggcttg ctcgttttca atggcgctgc aaagctgata atcatcttca tggtggaact
    39061 cttcacactg acgcgcagtg cctgccatgc caaacagaat ccgttcctca ccgtttaata
    39121 acagctggat atcaatgtat tgttcgtgca attcggcttt tttctcaacg ggcgattgag
    39181 tattaaatgt catgacattc ataaagatat tgtcgccctg taattcgtaa cggccaggcg
    39241 ctttttcttg cggtctggca gctaatgcca acgttaacgc gtcctgtaac gcaggatgta
    39301 atccggcaga cggtaatgac tgtacttcac ccatcatcat aatatttctc cctgggccaa
    39361 cagcgcagcc ccaagtaaac ctgcatcgtg gcggtagtgc gccgccagta aatcaacatg
    39421 aaatgccgct ggctcctgcg ccagatacgt ttccaccagc gccagatacc cttctgccag
    39481 accaacgctg ccaccgacca ccacgcactg gcaatcagtt gtggctttaa tatcagcgat
    39541 cagccttgca agcgtacgtg cggagcggtg aatcagttgc tgcgcctgct catcgccctg
    39601 cccggcgcgc gtgaaaatag ttttcgcatt cgcaccagcc aactccccct gcgctgcagc
    39661 tgcaatgccg cgaccagaag caatcgcttc cacgcaacct gtgcgtccac agccgcagac
    39721 tggaccatgc ggatcggcaa gcgtatgccc gatatgcccc gccagaccgc cagggccggt
    39781 acgcagttta ccgccgctca ctacaccgcc gccaacgccg gtggaaacag tgataaacac
    39841 catatcggtt atatcgccat ccagcgcctg atactccgcc catgctgcgg cctgcgcatc
    39901 attaatggca atggtcggca aattggtaag ttgctccagc gttttgacta acggaaagtg
    39961 taacaatcca ccgagattat gcggattcag cgccagcaag ctgccgtcgc gaataattcc
    40021 ggttgaagcg atagcaaccc gctgcgcatg tgcttgcaac ggagagacta atgcggataa
    40081 ggcatcacgc agcgcctgcg gagtctggct ggcaggcgta ggaagttctc ggcgatcgcg
    40141 gatctgcccg tcagcgccaa tcagcgcggc ggcaagttta gtaccgccga tatcaatcgc
    40201 cagtgtggtc atagcaccgc ctttttcatc gctgtgttgt accactgaca aatgtgttca
    40261 agacgcgtaa tggcagaacc gaccgtcacc gcccacgcgc cgtggcgcat cgcatccgcc
    40321 gcctgagcag gcgtgttgta acgcccttcg gcaatcaccc ggcatccggc gtcactcaac
    40381 gttttcacca gcgccagatc cggctcttct ggcgtttcag gcgtggtata gccagaaagc
    40441 gtagtgccaa taatttcggc tcccagcttt tggcatgcca ggccgtcttc cggcgttgag
    40501 cagtcggtca tcgccagtaa accgtgatgg tgaatacgtg ccagcagcgt ttcaacaggc
    40561 accggacgcg gtcggtcagt gccgtcaatg gcgataatgt ccgcgcccgc ctgcgccagc
    40621 gcatcaacat cttcaatata ggccgtgatg cgtaccggag aatcctccag atcgcgcttc
    40681 acaatcccaa taatcggcac gctcaccacc gcacgcgtgg cttgcagatt tgccacacct
    40741 tcaatacgaa tggcaaccgc gcccgcctgc tctgccgcta atgccatggc agcgacgatt
    40801 tcgggtttat cgagcgggct gtccggaacc ggctggcagg agacaatcag gccaccgtta
    40861 gcagcgattt tttgatccag ttgtgcaagt aacgacatac atcttccctt agcgaaaggc
    40921 ccggtacaga gaccgggcaa caggattaac ttttggtttt gactaaatcg tttttggcgc
    40981 tgccaaatgg cacggcaccg ctaaatggtt tgccgtcgat agcgtcatga gtacgcaatg
    41041 cttccgggcg caaccaacgc tgaacacgag aaggcatatc cagaccaatc agcaggatca
    41101 ccacaaacgt taaactaaac gagagcgagc caagtgccgt tcccagatcc agacgttgag
    41161 cgatcaacgc gccgaggatt ggagctagtg caccgcccag tgcgccaacg ttgtaggtaa
    41221 agcccagccc cgccgcacgc tggtcggtat cgaaataacc gccgatcagt ttcggtaaaa
    41281 tccctgagat gccctgacca agcatttgct ggaagaacag taacaaaccg agcacccaga
    41341 cgtttgcgcc gccaatcgca aataccggaa taatcagcag ctgcgaggcc agcagactac
    41401 aaacgtacgc tttgcgggtt cccagccagt caccgaggaa gccaccgacg cagcacccca
    41461 ccgccgcgcc aaagccacta aagaacagca cattggcgac agtatgcggg tcataagcca
    41521 gatcggtttt cagatacgtt ggcagcagcg cctgaatcgg ccatgagtag aggaaagcga
    41581 acaacacgac caccatcagc attacgcccg ttggccagcg tttgcccgta ctctgcacca
    41641 taaagctgat aaagattgcg gcgcataaca gcccgagtac agcgacgatt gcggcatttt
    41701 gcaggttacc ggcgaagcag aaccacagcg cagtagccgc cgccagcgtc attacgatat
    41761 tggcaatgcg atgttcacca cggtaaagaa tgtctaccat cgtgcgtact ggtgctttac
    41821 ccccgtgttt ctctttccag tcttccgctt ccgggatgtt tttacgcagc cagagagcaa
    41881 agatgattgg caaaatgccg ataaagaaca gcgcacgcca gccccagacc ggaaccacca
    41941 gactatagac ctgagcggca acgacggccc ccactgagaa gcctgaaatc agaaaaccac
    42001 tggctttgtt acgcagatgc tttggccagc tttcaatgac ataggtggcg ctggaaccgt
    42061 attcacccgc catccccatg ccgatgacca gacgagcgat aaacatggtg atgtagcctg
    42121 gcgcaaagcc gcaggccagc gtcccggccg agaagagaac gatgctggtg accattgcca
    42181 gacgacgccc gtagcggtca cccatagcgc cgagcatcag gccgccgaac cagcgagaga
    42241 taaaggctgc ggagatcaga cttgccgcct gcaccgtcgt cagcccgaat tcaccctgta
    42301 cttcggtgag taccagggcg attaaaacga aatcaaaacc gtcaagcaga tatcccaacc
    42361 aggcagcgga aaatgcccgc cattgtgcgc ggttaagatg gcgataccac gggatattct
    42421 gggttgtagt actcattgtg agtctcccgc ggtgggcgat gcccacacgc tttggtatga
    42481 aaattgtagg gtacagatgc gtttatttcc cctcacccgg taggggcgag cgaggggaaa
    42541 caactcaccc gcgctcttgc atcaactgct gagccagcgc cttcagttct ggcagatatt
    42601 tttcatctac cggaccaaac ggtttgcggc acagcggcac agaaacgaca tccatataat
    42661 gcaggacagt tttcagccca cggaatacgc cagttttgat cagtaaatca atgactttgt
    42721 tgcactcagt ttgcagtttc tgcgcggtct ggatatcgcc ttctttcagc gccttcacga
    42781 tcccctgata acgccagccc atgatgttgt aagtgctgcc gataccgccg tctgcgcccg
    42841 ccagaagacc agaggcgaag atttcgtcgt aaccgttata gagcaccaga tcaggatgtt
    42901 cacgacggat ctgctccatc tgatagagat cgccagaggt ctgtttcagc gcacctacgc
    42961 caggcagcgt aaccaaggtg ttgatctgat ccagggtcag ttttaccccg ctcagggctg
    43021 gaatgttgta caccaccatc ggcaacccat ccgctgaatc aataattgcc cgatagtgat
    43081 cgcagtgttc ttcaaagctg aaaggatagt agaacggcgt gacggcggag acggcatcga
    43141 agccataacg tttagccgat gccgcaagtt gttggctttc ggcggtgctg acgcaaccga
    43201 catgggcgat gagtttaatc ttacctttcg cctcttcagc gacgatttcc agtacctgtt
    43261 cgcgctcgga aaggctttgt acaaaggctt cgccggtcga accacccacg tacaaaccgt
    43321 cgatgccctg ctgaatattg aactgaacca ggcggcgcag actcgcttta tccagtgctt
    43381 gttgttggtc aaaaggagtc aggagtgcag ccattacgcc acgtaaattc gttgccataa
    43441 atacctctga agtgatgctt gtctgataaa cgatatacct ttatacctgt tataccagat
    43501 caattaagca acaccccata cagaaagctt ataatgcgat ctgcttcact aaagtggcat
    43561 tatttctttt tgttggtggt ctgaccgaag gcgtgccagg tagcagagac gctgttgaga
    43621 tgcgattgca acgcgcgatc ggcttcgtca ggatcatgac ggcggatcgc gtcaacgatc
    43681 gcaatatgct gttgatagct aacgttgtta tgctcatgca gagcctgatc ggtcacggtt
    43741 ggacgtgcgg cgattagcca gtcgagcagg gcaacgtgga tcgccatgaa gattgggtta
    43801 cccgggatct ccgccagaac gcggtggaaa tcaacgtctg agcgaatgaa tgccgcgttg
    43861 ttatccagcg actgactgtt gatttccagt gcttttgcca gcaaatcgat ttgctcatcg
    43921 gtggcgtgtt cagccgcata gcgcaccagg ctggattcaa agaacagacg taattgttcg
    43981 aaatgggcaa tcccaccagg atgagaaagg aaatctttcg ccatgccgga aagctcgccg
    44041 atgatggtgt ccgcagaagg acgtgagacg cgggcacgtt cgccgttgtt tatttgcacc
    44101 agacctttgc gttttaacgc tgccagcgct tcgcgcaccg aaggacgccc gacgttaaag
    44161 aacgccatca gttcgcgttc agacggtaat tgttcacctt cgccaaattc acgacggcgg
    44221 atcatctgtt ccagctcttc ttccaccatt tcggagagtt ttttacgcgc cagcgggcgg
    44281 ctgcgcaagt tgcgcccgat agtggtagta gaatcttcag cttgcggatc aaatgcgttc
    44341 ataggactca ttcaggaaaa tgcgttagcg gataataacg acgctatcgt agcacagtat
    44401 caaagtcagg cgaaaagatg acagatagca aaacaggcca catgggcctg ttttcatttt
    44461 acttcacaac gcgtaatgcc ggtcgaccac cgcgtggtgg ctgcggaggt tcatcgtcag
    44521 gatgagtttc atcatcgtga tctggcttgt cgccatcgat aaccgacata acggtttcgt
    44581 tgtctgccga tgcctcttca tcattcatga tgctggcatc ttcatcgtag gcagcttcag
    44641 gctcaaacat cgtgcctgcg ccattttcac gggcgtagat agccagcacg gcagccagcg
    44701 gcacagaaac ctgacgcgga atgccaccaa agcgcgcgtt aaagcgcacc tcatcattcg
    44761 ccagttccag attgccgaca gcacgcggcg caatgttgag tacgatttgc ccgtcacgcg
    44821 catattccat aggaacctgc acgccaggga gcgtcacatc caccaccagg tgcggcgtga
    44881 gctggttatc cagcaaccac tcatagaatg cacgcagcag atagggacga cgtggtgtta
    44941 gctgtgacaa atccatacag attaactccg gcccagacgc atttcacgtt ctgcttcagt
    45001 taaagaagca aggaaagagt cacgttcaaa gacgcgggtc atatagcctt tcagctcttt
    45061 cgcacccggg ccgctgaact cgatgcccag ttgcggcaga cgccacagca gaggagcaag
    45121 gtagcaatcg accaggctga actcatcgct caggaagtac ggcttctgac cgaagaccgg
    45181 cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg gcatcggctt cagaagctga
    45241 accgttgatg atggtgttca tcagcgtgta ccagtctttt tcgatgcgat gcatgtacag
    45301 acggctttca ccgcgagcta ccgggtaaac aggcatcagt ggcggatgcg ggaaacgctc
    45361 atccagatat tccataatga tgcgagattc ccacagggtc agctcacgat ccaccagggt
    45421 cggaacgctc tgattcgggt tgaggtcaat cagatcctga ggcggattgt ccttttccac
    45481 gtgttcgatc tcgaaactta cacctttctc agccagcaca atgcggacct gatggctata
    45541 gatgtcagta ggaccggaaa acagcgtcat taccgaacgt ttgttggcag cgacagccat
    45601 gaaaacctcc aggtatagtc agaattttta ctgctaccag ccaccaggtg gccagtcaga
    45661 agttgtgtta cccagtaagg aacgactctc tttgttcgaa aatcaaacaa aaaatgagca
    45721 atacccgaca tttgggcaga aaattggatg atagtttacc agattttgcg acctttgtgg
    45781 tgagtcgatg ccggaaatgg ggaaaaagag atgcgcttta gtctgaaata gttgacttag
    45841 tcccttattg gcgatgtggt ttttgtttta cctgtctgtc aggtggcagc aaaaagcaac
    45901 tttccatttt ttacgctgat gcagatttta gccataaaaa aacccgccga agcgggtttt
    45961 ttcgcaaatt gttttctgcc ggagcagaag ccaattaacg tttggagaac tgcggacgac
    46021 gacgtgcttt acgcagaccg actttcttac gttcaacctg acgagcgtca cgagtaacga
    46081 agccagcttt acgcagttca gaacgcaggg actcgtcgta ttccatcaga gcgcgggtga
    46141 taccgtgacg gatcgcacca gcctgaccag agataccacc acctttaacg gtgatgtaca
    46201 ggtccagttt ctcaaccatg tcgaccagtt ccagcggctg acgaactacc atgcgagcag
    46261 tttcacgacc gaagtactgt tccagagaac gttggttgat tacgatttta ccgttgcccg
    46321 gtttgatgaa aacgcgagct gcggaacttt tgcggcgacc agtgccgtag tattgatttt
    46381 cagccattgc ctataatccc gattagatgt caagaacttg cggttgctgt gccgcgtggt
    46441 tgtgctcgtt acccgcgtaa actttcagtt tacggaacat agcacgaccc agcgggcctt
    46501 ttggcaacat gcctttaacc gcgatttcaa tcacacgctc aggacggcga gcaatcatct
    46561 cttcaaaggt cgcttgtttg ataccaccga tgtggccggt gtggtgatag tacactttgt
    46621 cagtacgctt gttgccggtt acagcaactt tgtcagcgtt cagaacgatg atgtaatcac
    46681 cggtatctac gtgcggagtg tattccgctt tgtgcttacc gcgcaggcga cgagccagtt
    46741 cagtagccag acggcccaga gttttaccgg tcgcgtcaac aacataccag tcgcgtttta
    46801 cggtttctgg tttagctgta aaagttttca ttaaaagctt acccaataaa tagttacacg
    46861 ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca cgacaaagtc cggcaaacct
    46921 accccttcga atagcctatg ccagcacaca aaaagtttcg ggaaaaaaaa ctttcttgta
    46981 acgtggggtc gcaggattat agagaagtcg gggtcaaaga tcgacccctt tttgtgattt
    47041 gtggcaggtt ttaccccgcc aaatgctcgc gcttcaaata ctcttcgctt tgcatctctt
    47101 gcagacgtga caggcaacgc tggaactcaa acttcagccg ctcgccctga taaatttcat
    47161 acagcggcac ttctgcactc accactaatt tgacatggcg ctcgtaaaac tcatccacca
    47221 gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt cataactggt acatcaaaca
    47281 acatgaccgt gtgaaagagg cgtgagagcg caatatagtc atgctgactg cgggcgtcga
    47341 cgcacagcgt agtaaaagaa accgccagcg tctggttctc gacgcccatt gtcgccaatg
    47401 gccgatggtt gatttctaac gtcggtgaat tttctcgttt cgcccccgcc agcgccaacc
    47461 atagtttatc catttgcgcc cgggtttcat cgttgagggg cgaaagccac agatgcgcct
    47521 gagtgagtgt acgcaggcga taatcaacac cagcgtccac gttcattaca tcacaatgct
    47581 gtttaatggc atcgattgca ggcaaaaaac gcgcacgttg caggccattt cgataaagtt
    47641 cgtccggcgg aatatttgac gtcgctacca gggtaatacc gcgggcgaac agggctttca
    47701 tcagaccgcc aagtagcatg gcatcggtaa tatcagaaac aaaaaattcg tcaaaacaga
    47761 gcacgtcagt ttctgcttta aagcgatcgg caataatttc cagcggatcg gtctgcccct
    47821 gcaaagcagt tagctcttca tgcacccgca gcataaaacg gtgaaagtgc aggcgctgtt
    47881 tccgctctcc cggcagactt tgatagaaaa ggtccatcag ccaggttttc ccgcgtccta
    47941 caccgcccca catatataag ccacgcactg gcatatgctt tgtgtcttcg cgtttacccc
    48001 acagcttacc gacccgcgcc attagcccac tcgtcctggg ggctggtggc gtgctattga
    48061 cgagttcctt ataaataatt tccaggcggc tgacggcctc tttttgaacg tcgtcgggtt
    48121 gatggctgcc ttcattgagc gccttcaggt attgcgatgt tggggtaacg ctttgcatga
    48181 tcttattgtt attccttgaa taatcggtgc gccgttgttc acggttgacg aaaaaaaggc
    48241 cgttctacac tacgcgatat gctgtcggga ttccacttct gtggaattaa cggttatagt
    48301 ggcataatca gccgcaggca tggagcctga agccaacacc ctacggaaac aaaagacaac
    48361 gggagatgtt catgacctgg gaatatgcgc taattgggtt agtcgtcggc atcattattg
    48421 gtgctgtggc catgcgtttt ggtaatcgta aactacgcca gcaacaggcg ttgcagtacg
    48481 aactggaaaa gaataaagct gaactggacg agtatcgcga agagctggtt agccactttg
    48541 cccgcagcgc ggaattactg gataccatgg cgcacgacta tcgccagctg tatcagcaca
    48601 tggcgaaaag ctccagtagc ctgctgccgg aactgtctgc ggaagcaaac ccgttccgta
    48661 atcgtctggc agagtctgaa gccagcaacg atcaggcacc ggtgcagatg cctcgcgact
    48721 attctgaagg cgcatccggc ctgctgcgta ctggcgcgaa gcgcgactaa tttatttttg
    48781 gggcgcagcc attgcgccct cctcttctct ccctccccga ctatcattta atctggtgtc
    48841 tcattgttag ccgtctgaaa attcaataac atcaaactgt tttgaatctc ttttcttatc
    48901 attcaggtac gagagcagga ataatgaaaa aacaaaccca gctgttgagt gcattagcgt
    48961 taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt cgcgtcgatt ccaggccagg
    49021 ttgccgatca ggcccccctc cccagtctgg ctccaatgct ggaaaaagtg cttccggcag
    49081 tggtgagcgt acgggtggaa ggaacggcca gtcagggaca gaaaatcccg gaagaattca
    49141 aaaagttttt tggtgatgat ttaccggatc aacctgcaca acccttcgaa ggtttaggct
    49201 ccggtgtcat catcaacgcc aataaaggct atgtgctgac caacaaccat gtgattaatc
    49261 aggcacagaa aatcagtatt cagctcaatg atgggcgcga gtttgatgca aaactgattg
    49321 gtagcgatga ccagagcgat atcgccctgt tgcaaattca aaacccgagc aaattaacgc
    49381 aaatcgctat tgccgactcc gataaattgc gcgtcggtga ttttgccgtg gcggtcggta
    49441 acccatttgg ccttgggcaa accgctacct ccgggattat atctgcgtta ggccgcagcg
    49501 gtctgaatct cgaaggtctg gaaaacttta ttcaaactga tgcctccatt aaccgcggca
    49561 attccggtgg tgcgctgtta aaccttaacg gtgagttaat tggcatcaac actgcaatcc
    49621 ttgcgcctgg cggcggtagc gtcgggattg gatttgccat tcccagtaat atggcgcgaa
    49681 cactggcgca gcagcttatc gactttggag aaatcaaacg cggcctgtta ggtatcaaag
    49741 gcaccgagat gagtgccgat atcgccaaag ccttcaacct tgacgtgcag cgtggcgcgt
    49801 ttgtcagcga agtgttgcca ggttctggct cggcgaaagc gggcgtcaaa gcgggcgata
    49861 ttattaccag cctcaacggc aaaccgctga atagctttgc tgagttgcgc tctcgtatcg
    49921 cgaccaccga gccgggcacg aaagtgaagc ttggcctgct gcgtaacggc aaaccactgg
    49981 aagtagaagt gacgctcgat accagcacct cttcgtcggc cagcgctgaa atgatcacgc
    50041 cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa agatggtggc aaaggtatta
    50101 agatcgatga ggttgtcaaa ggaagcccag ctgctcaggc tggcttgcaa aaagacgatg
    50161 tgatcattgg cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt aaagtgctgg
    50221 cggcaaaacc ggccataatc gccttgcaaa tcatacgcgg caatgaaagc ctctacctac
    50281 taatgcgtta atgtcgtaaa ccgggcatca ggctgacgtg tgatgtccgg ttaactcgtg
    50341 gtatgctgct gccgttccct tttttaacga tgcctccatc atgtttgtga agctcttacg
    50401 ttccgttgcg attggattaa ttgtcggcgc tattctgctg gttgccatgc cttcgctgcg
    50461 cagccttaac ccgctttcca ctccgcaatt tgacagtacc gatgaaacgc ctgccagcta
    50521 taatctggcg gttcgccgcg ccgcgccagc ggtggttaac gtttataacc gcggtttgaa
    50581 caccaactct cacaaccagc ttgagatccg caccctggga tctggtgtaa tcatggatca
    50641 acgcgggtat atcatcacca ataaacacgt gatcaacgac gccgatcaga tcatcgtcgc
    50701 cttacaggat ggacgtgttt ttgaagcgtt actggttggc tctgactctc taaccgatct
    50761 ggcggtgctg aaaattaacg ccacaggcgg tttaccaacg attccgatca atccacgccg
    50821 cgtaccgcac attggcgatg tggttctggc gatcggtaac ccgtacaacc tcgggcagac
    50881 cattacccag gggattatca gtgccacggg tcgaatcggt ctgaacccga ccgggcggca
    50941 aaacttcctg caaaccgatg cttccattaa ccacggtaac tctggcggcg cgctggtgaa
    51001 ctcgctgggc gaactgatgg gcattaacac gctgtcgttt gataagagta acgatggcga
    51061 aacgccggaa ggtatcggct ttgcgattcc tttccagtta gcgaccaaaa ttatggataa
    51121 actgatccgt gatggccgtg tgatccgtgg ctacattggc attggtggac gtgagatcgc
    51181 cccattacat gcgcagagtg gcggcatgga tcaactgcaa gggatcgtgg ttaacgaagt
    51241 gtcgcctgat ggcccagcgg caaatgcagg catccaggtc aacgatctga ttatctctgt
    51301 gaataacaaa cccgctattt ccgccctgga gacaatggat caggtcgcgg aaattcgccc
    51361 tggttcggtg atcccggttg tcgtgatgcg tgacgataaa cagttaacat tgcaggtcac
    51421 cattcaggaa tatccggcga ccaactaagg tcagcgtaaa aaaacaaaaa ccggagtctg
    51481 tgctccggtt ttttattatc cgctaatcaa ttacttatta acgaactctt cgcccagggc
    51541 gatatctttc ttcagcgtat ccagcatacc ttccagcgcg ttctgttcaa atgcgctcag
    51601 agtaccgata gatttacgct cttccacgcc gtttttaccc agcagcagcg gttgagagaa
    51661 gaaacgagcg tactgaccgt cgccttcaac gtaggcacat tcgacaacgc cttgttcgcc
    51721 ctgcagtgcg cgaaccagag acagaccaaa acgtgcagct gcctggccca tagacagggt
    51781 tgcagacccg ccaccggctt tcgcttcaac cacctcagta cccgcgttct ggatacgttt
    51841 ggtcagatca gccacttcct gctcggtaaa actaacgcca ggaacctgtg acagcagcgg
    51901 cagaatggta acaccagagt gaccaccaat aaccggcact tcaacttcgc ctggctgttt
    51961 gcctttcagt tccgcaacaa aggtgttgga acgaatgata tccagcgtgg taacgccgaa
    52021 cagtttgttt ttgtcataaa caccggcttt tttcagcact tcagcagcaa tcgcaactgt
    52081 agtgttaacc gggttagtga taataccaat gcacgctttc gggcaggttt tcgcaacttg
    52141 ctgtaccagg tttttcacga taccggcgtt aacgttaaac aggtcggaac gatccatacc
    52201 cggtttacgc gctacgcctg cagagataag aacgacatct gcaccttcca gcgccggagt
    52261 cgcatcttca cctgaaaaac ctttgatttt cacagcagta gggatatggc tcagatcgac
    52321 agccacaccg ggagtcactg gagcgatatc atacagagag agttctgaac ctgaaggcag
    52381 ttgggttttt aacagtagtg caagcgcctg gccaataccg ccagcagcgc cgaggactgc
    52441 gactttcatc ctaaactcct tattatattg ataaactaag atatgttgct ccgctgccgc
    52501 gaccttaatc cacaaaattg ccgtttacaa tgaccacatc tcaagaatgt gtagtcacgc
    52561 aagtttagcg tttatgcatt taattgccgt aatcaggaac ctaacttacg taattaacag
    52621 tcacttatgc attagcgcaa cattccaaca ggtggtgaca atatacccta ccgctcagcc
    52681 aaaacaatat caatttgata acaattaatt tacttttaag cagaatttgc aggccgtgac
    52741 gcaggcatgt ttctcaataa cgaaatttga taaaatcccg ctctttcata acattatttc
    52801 agccttcttc agggctgact gtttgcataa aaattcatct gtatgcacaa taatgttgta
    52861 tcaaccacca tatcgggtga cttatgcgaa gctcggctaa gcaagaagaa ctagttaaag
    52921 catttaaagc attacttaaa gaagagaaat ttagctccca gggcgaaatc gtcgccgcgt
    52981 tgcaggagca aggctttgac aatattaatc agtctaaagt ctcgcggatg ttgaccaagt
    53041 ttggtgcggt acgtacacgc aatgccaaaa tggaaatggt ttactgcctg ccagctgaac
    53101 tgggtgtacc aaccacctcc agtccattga agaatctggt actggatatc gactacaacg
    53161 atgcagttgt cgtgattcat accagccctg gtgcggcgca gttaattgct cgcctgctgg
    53221 actcactggg caaagcagaa ggtattctgg gcaccatcgc tggcgatgac accatcttta
    53281 ctacccctgc taacggtttc accgtcaaag agctgtacga agcgatttta gagctgttcg
    53341 accaggagct ttaatctctg ccccgtcgct tctggcggcg gggaaaatgt tgcttttgcc
    53401 tatcaaccca ctgctttccc ctgcgattca tttaacgaat agtgcgtttt actgcgacat
    53461 atcattcaca caatgaatac atatagtaaa aaa
//
LOCUS       DACADF010000011        50264 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.011, whole genome shotgun sequence.
ACCESSION   DACADF010000011 DACADF010000000
VERSION     DACADF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.011"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            198..1145
                     /gene="cyoA"
                     /locus_tag="ISB30_000655"
     CDS             198..1145
                     /gene="cyoA"
                     /locus_tag="ISB30_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HAP1988333.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1167..3158
                     /gene="cyoB"
                     /locus_tag="ISB30_000656"
     CDS             1167..3158
                     /gene="cyoB"
                     /locus_tag="ISB30_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HAP1988334.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQIYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3148..3762
                     /locus_tag="ISB30_000657"
     CDS             3148..3762
                     /locus_tag="ISB30_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAP1988335.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3762..4091
                     /locus_tag="ISB30_000658"
     CDS             3762..4091
                     /locus_tag="ISB30_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAP1988336.1"
                     /translation="MSHSNASSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4103..4993
                     /gene="cyoE"
                     /locus_tag="ISB30_000659"
     CDS             4103..4993
                     /gene="cyoE"
                     /locus_tag="ISB30_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAP1988337.1"
                     /translation="MIFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5142..6506
                     /locus_tag="ISB30_000660"
     CDS             5142..6506
                     /locus_tag="ISB30_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1988338.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHVLFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(6634..7125)
                     /gene="yajQ"
                     /locus_tag="ISB30_000661"
     CDS             complement(6634..7125)
                     /gene="yajQ"
                     /locus_tag="ISB30_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAP1988339.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7293..8204
                     /gene="panE"
                     /locus_tag="ISB30_000662"
     CDS             7293..8204
                     /gene="panE"
                     /locus_tag="ISB30_000662"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP1988340.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8167..8757
                     /gene="yajL"
                     /locus_tag="ISB30_000663"
     CDS             8167..8757
                     /gene="yajL"
                     /locus_tag="ISB30_000663"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAP1988341.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(8811..10259)
                     /gene="thiI"
                     /locus_tag="ISB30_000664"
     CDS             complement(8811..10259)
                     /gene="thiI"
                     /locus_tag="ISB30_000664"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP1988342.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     NWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10465..10707
                     /gene="xseB"
                     /locus_tag="ISB30_000665"
     CDS             10465..10707
                     /gene="xseB"
                     /locus_tag="ISB30_000665"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP1988343.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            10707..11606
                     /gene="ispA"
                     /locus_tag="ISB30_000666"
     CDS             10707..11606
                     /gene="ispA"
                     /locus_tag="ISB30_000666"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAP1988344.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSFDTSALEALADYIIQRNK"
     gene            11631..13493
                     /gene="dxs"
                     /locus_tag="ISB30_000667"
     CDS             11631..13493
                     /gene="dxs"
                     /locus_tag="ISB30_000667"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP1988345.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            13548..14522
                     /gene="yajO"
                     /locus_tag="ISB30_000668"
     CDS             13548..14522
                     /gene="yajO"
                     /locus_tag="ISB30_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAP1988346.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKVGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(14576..15094)
                     /gene="pgpA"
                     /locus_tag="ISB30_000669"
     CDS             complement(14576..15094)
                     /gene="pgpA"
                     /locus_tag="ISB30_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP1988347.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAVILY
                     FIGHHWPLGILS"
     gene            complement(15072..16049)
                     /gene="thiL"
                     /locus_tag="ISB30_000670"
     CDS             complement(15072..16049)
                     /gene="thiL"
                     /locus_tag="ISB30_000670"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAP1988348.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(16127..16546)
                     /gene="nusB"
                     /locus_tag="ISB30_000671"
     CDS             complement(16127..16546)
                     /gene="nusB"
                     /locus_tag="ISB30_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP1988349.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(16566..17036)
                     /gene="ribE"
                     /locus_tag="ISB30_000672"
     CDS             complement(16566..17036)
                     /gene="ribE"
                     /locus_tag="ISB30_000672"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAP1988350.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17125..18228)
                     /gene="ribD"
                     /locus_tag="ISB30_000673"
     CDS             complement(17125..18228)
                     /gene="ribD"
                     /locus_tag="ISB30_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAP1988351.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(18232..18681)
                     /gene="nrdR"
                     /locus_tag="ISB30_000674"
     CDS             complement(18232..18681)
                     /gene="nrdR"
                     /locus_tag="ISB30_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAP1988352.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            18832..19371
                     /locus_tag="ISB30_000675"
     CDS             18832..19371
                     /locus_tag="ISB30_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAP1988353.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            19670..20554
                     /locus_tag="ISB30_000676"
     CDS             19670..20554
                     /locus_tag="ISB30_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAP1988354.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(20592..20939)
                     /locus_tag="ISB30_000677"
     CDS             complement(20592..20939)
                     /locus_tag="ISB30_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAP1988355.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21069..22040)
                     /gene="secF"
                     /locus_tag="ISB30_000678"
     CDS             complement(21069..22040)
                     /gene="secF"
                     /locus_tag="ISB30_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAP1988356.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22051..23898)
                     /gene="secD"
                     /locus_tag="ISB30_000679"
     CDS             complement(22051..23898)
                     /gene="secD"
                     /locus_tag="ISB30_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAP1988357.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(23926..24258)
                     /gene="yajC"
                     /locus_tag="ISB30_000680"
     CDS             complement(23926..24258)
                     /gene="yajC"
                     /locus_tag="ISB30_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP1988358.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24281..25408)
                     /gene="tgt"
                     /locus_tag="ISB30_000681"
     CDS             complement(24281..25408)
                     /gene="tgt"
                     /locus_tag="ISB30_000681"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP1988359.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(25463..26533)
                     /gene="queA"
                     /locus_tag="ISB30_000682"
     CDS             complement(25463..26533)
                     /gene="queA"
                     /locus_tag="ISB30_000682"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP1988360.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            26626..27207
                     /gene="acpH"
                     /locus_tag="ISB30_000683"
     CDS             26626..27207
                     /gene="acpH"
                     /locus_tag="ISB30_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HAP1988361.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            complement(27212..29029)
                     /gene="malZ"
                     /locus_tag="ISB30_000684"
     CDS             complement(27212..29029)
                     /gene="malZ"
                     /locus_tag="ISB30_000684"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAP1988362.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIELSCGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARQEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGIRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29185..30558)
                     /gene="proY"
                     /locus_tag="ISB30_000685"
     CDS             complement(29185..30558)
                     /gene="proY"
                     /locus_tag="ISB30_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAP1988363.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(30634..31953)
                     /gene="brnQ"
                     /locus_tag="ISB30_000686"
     CDS             complement(30634..31953)
                     /gene="brnQ"
                     /locus_tag="ISB30_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAP1988364.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(32360..33655)
                     /gene="phoR"
                     /locus_tag="ISB30_000687"
     CDS             complement(32360..33655)
                     /gene="phoR"
                     /locus_tag="ISB30_000687"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAP1988365.1"
                     /translation="MLERLSWKRLVLELLFCCLPAFILGAFLGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFIIPERLIAKNSD"
     gene            complement(33713..34402)
                     /gene="phoB"
                     /locus_tag="ISB30_000688"
     CDS             complement(33713..34402)
                     /gene="phoB"
                     /locus_tag="ISB30_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAP1988366.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            34592..35794
                     /gene="sbcD"
                     /locus_tag="ISB30_000689"
     CDS             34592..35794
                     /gene="sbcD"
                     /locus_tag="ISB30_000689"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAP1988367.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHLVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQLIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            35791..38934
                     /gene="sbcC"
                     /locus_tag="ISB30_000690"
     CDS             35791..38934
                     /gene="sbcC"
                     /locus_tag="ISB30_000690"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAP1988368.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKDEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEQA
                     QPQLAALSLAQPARNLRPHWERIAEHSTALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASAQAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNKMRHRYKEKMQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLTQHQQHRPGGLSLTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            39060..40244
                     /gene="araJ"
                     /locus_tag="ISB30_000691"
     CDS             39060..40244
                     /gene="araJ"
                     /locus_tag="ISB30_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAP1988369.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAIFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(40513..41421)
                     /gene="mak"
                     /locus_tag="ISB30_000692"
     CDS             complement(40513..41421)
                     /gene="mak"
                     /locus_tag="ISB30_000692"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAP1988370.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVHKAKHGDSSGVRGAAWLWPQE"
     gene            41546..42457
                     /gene="rdgC"
                     /locus_tag="ISB30_000693"
     CDS             41546..42457
                     /gene="rdgC"
                     /locus_tag="ISB30_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAP1988371.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQVRKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(42538..42618)
                     /locus_tag="ISB30_000694"
     CDS             complement(42538..42618)
                     /locus_tag="ISB30_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988372.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(42615..42956)
                     /locus_tag="ISB30_000695"
                     /pseudo
     CDS             complement(42615..42956)
                     /locus_tag="ISB30_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(43104..43388)
                     /gene="ppnP"
                     /locus_tag="ISB30_000696"
     CDS             complement(43104..43388)
                     /gene="ppnP"
                     /locus_tag="ISB30_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAP1988373.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(43460..44137)
                     /locus_tag="ISB30_000697"
     CDS             complement(43460..44137)
                     /locus_tag="ISB30_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAP1988374.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(44395..44586)
                     /gene="yaiA"
                     /locus_tag="ISB30_000698"
     CDS             complement(44395..44586)
                     /gene="yaiA"
                     /locus_tag="ISB30_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAP1988375.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(44636..45160)
                     /gene="aroL"
                     /locus_tag="ISB30_000699"
     CDS             complement(44636..45160)
                     /gene="aroL"
                     /locus_tag="ISB30_000699"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAP1988376.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(45343..45801)
                     /locus_tag="ISB30_000700"
     CDS             complement(45343..45801)
                     /locus_tag="ISB30_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP1988377.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            45921..46730
                     /gene="proC"
                     /locus_tag="ISB30_000701"
     CDS             45921..46730
                     /gene="proC"
                     /locus_tag="ISB30_000701"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP1988378.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHEQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(46747..47862)
                     /gene="adrA"
                     /locus_tag="ISB30_000702"
                     /gene_synonym="dgcC"
     CDS             complement(46747..47862)
                     /gene="adrA"
                     /locus_tag="ISB30_000702"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAP1988379.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(47964..48284)
                     /gene="psiF"
                     /locus_tag="ISB30_000703"
     CDS             complement(47964..48284)
                     /gene="psiF"
                     /locus_tag="ISB30_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAP1988380.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(48403..49818)
                     /gene="phoA"
                     /locus_tag="ISB30_000704"
     CDS             complement(48403..49818)
                     /gene="phoA"
                     /locus_tag="ISB30_000704"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HAP1988381.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(49919..50179)
                     /gene="iraP"
                     /locus_tag="ISB30_000705"
     CDS             complement(49919..50179)
                     /gene="iraP"
                     /locus_tag="ISB30_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAP1988382.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT        12235 a        13410 c        12689 g        11930 t
ORIGIN      
        1 cttctgcact tccctttcgt tataacgccc ttttgcaaca gcttcttaaa atcaacttga
       61 tatgttttgc aacatatgtg acctgatagc caaatccaag taacaggaat ttaatcatgt
      121 ttacagtaat gtaaccttcc cgtaaaatgc ccacacactt taaacgccac cagatcccgt
      181 ggaattgagg tcgttaaatg agactcagga aatacaataa aagtttggga tggttgtcat
      241 tatttgcagg cactgtattg ctcagtggct gtaattctgc gctgttagat cccaaaggac
      301 agattggtct ggagcaacgt tcactgatac tgacggcatt tggcctgatg ttgattgtcg
      361 ttattcccgc aatcttgatg gctgttggtt tcgcctggaa gtaccgtgcg agcaataaag
      421 atgctaagta cagcccgaac tggtcacact ccaataaagt ggaagctgtg gtctggacgg
      481 tacctatctt aatcatcatc ttccttgcgg tactgacctg gaaaaccact cacgctcttg
      541 agcctagcaa gccgctggca cacgacgaga agcccattac catcgaagtg gtttccatgg
      601 actggaaatg gttcttcatc tacccggaac agggcattgc aaccgtgaat gaaatcgctt
      661 tcccggcgaa cactccggtg tacttcaaag tgacctccaa ctccgtgatg aactccttct
      721 tcattccgcg tctgggtagc cagatttatg ccatggccgg tatgcagact cgcctgcatc
      781 tgatcgccaa cgaacccggt acttatgacg gtatctccgc cagctacagc ggcccgggct
      841 tctcaggcat gaagttcaaa gctattgcaa caccggatcg cgccgcattc gaccaatggg
      901 tcgcaaaagc gaaacagtcg ccgaacagca tgtctgacat ggcagcgttc gaaaaactgg
      961 ccgcgcctag cgaatacaac caggtggaat atttctccaa cgtgaaacca gacttgtttg
     1021 ccgatgtgat taacaagttt atggctcacg gtaagagcat ggacatgacc cagccagaag
     1081 gtgagcacag cgcacatgaa ggtatggaag gcatggacat gagccacgcg gaatccgccc
     1141 attaaagggg ttgaggaaga ataaagatgt tcggaaaatt atcacttgat gcagtcccgt
     1201 tccatgaacc tatcgtcatg gttacgatcg ctggcattat tttgggaggt ctggcgctcg
     1261 ttggcctgat cacttacttc ggtaagtgga cctacctgtg gaaagagtgg ctgacctccg
     1321 ttgaccataa acgtctcggt atcatgtata tcatcgtggc gattgtgatg ttgctgcgtg
     1381 gttttgctga cgccattatg atgcgtagtc agcaggccct tgcctctgcg ggcgaagcgg
     1441 gcttcctgcc acctcaccac tacgatcaga tcttcaccgc acacggcgtg atcatgatct
     1501 tcttcgtggc gatgcctttc gttatcggtc tgatgaacct ggtggttccg ctgcagatcg
     1561 gcgcgcgtga cgttgcgttc ccgttcctca acaacttaag cttctggttt accgttgttg
     1621 gtgtgattct ggttaacgtt tctctcggcg tgggcgaatt tgcgcagacc ggctggctgg
     1681 cctatccacc gctatcggga atagagtaca gtccgggagt cggtgtcgat tactggatat
     1741 ggagtctcca gctatccggt ataggtacga cgcttaccgg tatcaacttc ttcgttacca
     1801 ttctgaagat gcgcgcaccg ggcatgacca tgttcaagat gccagtattt acctgggcat
     1861 cactgtgcgc aaacgtactg attattgctt ccttcccaat tctgacggtt accgtcgcgt
     1921 tgttgaccct ggatcgctat ctgggcaccc atttctttac caacgatatg ggtggcaaca
     1981 tgatgatgta tatcaacctg atttgggcct ggggccaccc ggaagtttac atcttgatcc
     2041 tgcctgtatt cggtgtgttc tccgaaattg cggcaacctt ctcgcgtaaa cgtctgtttg
     2101 gttatacctc gctggtatgg gcaaccgtct gtatcaccgt gctgtcgttc atcgtctggc
     2161 tgcaccactt ctttacgatg ggtgcgggcg cgaacgtaaa cgccttcttt ggtatcacca
     2221 cgatgattat cgccatccct accggggtga agatcttcaa ctggctgttc accatgtatc
     2281 agggccgcat cgtgttccat tctgcgatgc tgtggaccat cggttttatc gttaccttct
     2341 cggtaggcgg tatgactggc gtgctgctgg cagtaccggg cgcggacttc gttctgcata
     2401 acagcctgtt cctgattgcg cacttccata acgtgatcat cggcggcgtg gtcttcggct
     2461 gcttcgcagg gatgacctac tggtggccta aagcgttcgg tttcaaactg aacgaaacct
     2521 ggggtaaacg cgcgttctgg ttctggatca tcggcttctt cgttgccttt atgccactgt
     2581 atgcgctggg cttcatgggc atgacccgtc gtttgagcca gcagattgac ccgcagttcc
     2641 acaccatgct gatgattgca gccagcggtg cggtactgat tgcgctgggt attctctgcc
     2701 tcgttattca gatatacgtt tctattcgtg accgcgacca gaaccgtgac ctgactggcg
     2761 acccgtgggg tggccgtacg ctggagtggg caacctcttc cccgcctccg ttctataact
     2821 ttgccgttgt gccgcacgtt cacgaacgtg atgcattctg ggaaatgaaa gagaaaggcg
     2881 aagcgtacaa aaagcctgac cactatgaag aaattcatat gccgaaaaac agcggtgccg
     2941 gtatcgtcat tgcggctttc tccaccatct tcggtttcgc catgatctgg catatctggt
     3001 ggctagcgat tgttggcttc gcaggcatga tcatcacctg gatcgtgaaa agcttcgacg
     3061 aggacgtgga ttactacgtg ccggtggcag aaatcgaaaa actggaaaac cagcatttcg
     3121 atgagattac taaggcaggg ctgaaaaatg gcaactgata ctttgacgca cgcgactgcc
     3181 cacgcgcacg aacacgggca ccacgatgca ggcggaacca aaattttcgg attttggatc
     3241 tacctgatga gcgactgcat tctgttctct atcttgtttg ctacctatgc cgttctggtg
     3301 aacggcaccg caggcggccc gacaggtaag gacattttcg aactgccgtt cgttctggtt
     3361 gaaactttct tgctgttgtt cagctccatc acctatggca tggcggctat cgccatgtac
     3421 aaaaacaaca aaagccaggt gatctcctgg ctggcgttga catggttgtt tggtgccgga
     3481 tttatcggga tggaaatcta tgaattccat cacctgattg ttaacggcat gggtccggat
     3541 cgcagcggct tcctgtcagc attctttgcg ctggtcggca cgcacggtct gcacgtcact
     3601 tccggtctta tctggatggc ggtgctgatg gtgcaaatcg cccgtcgcgg cctgaccagc
     3661 actaaccgta cccgcatcat gtgcctgagc ctgttctggc acttcctgga tgtggtttgg
     3721 atctgtgtgt tcactgttgt ttatctgatg ggggcgatgt aatgagtcat tctaacgcaa
     3781 gcagcggcgc atcccatggc agcgtaaaga cctacatgac aggctttatc ctgtcgataa
     3841 tcctgacggt aattccgttc tggatggtga tgacaggggc tgcctctccg gccgtaattc
     3901 tgggaacaat cctggcaatg gcagtggtac aaattctggt gcatctggtg tgcttcctgc
     3961 acatgaatac caaatcagat gaaggctgga atatgacggc gtttgtcttc accgtgctaa
     4021 tcatcgccat cctggttgtg ggctccatct ggattatgtg gaacctcaac tacaacatga
     4081 tgatgcacta agagcggcgg ttatgatatt taagcaatac ctgcaagtaa cgaaaccagg
     4141 catcatcttt ggcaacctga tctcggtgat tgggggattc ttgctggcct caaagggcag
     4201 cattgattat cccctgttta tctacacgct ggttggggtg tcactggttg tggcgtcggg
     4261 ttgtgtgttt aacaactaca tcgacaggga tatcgacaga aagatggaaa ggacgaagaa
     4321 tcgggtgctg gttaaaggcc tgatctctcc tgctgtctcg ctggtgtacg ccaccttgct
     4381 gggtattgct ggctttatgc tgctgtggtt tggcgcgaat ccgctggcct gctggctggg
     4441 ggtgatgggc tttgtggttt atgtcggcgt ttatagcctg tacatgaaac gtcattctgt
     4501 ctacggcaca ttgattggtt cgctctccgg cgctgcgcct ccggtgatcg gttactgtgc
     4561 ggtaaccggc gagttcgata gcggcgcagc gatcctgttg gctatcttca gcctgtggca
     4621 gatgcctcac tcttatgcca tagccatttt ccgctttaag gattatcagg cggcaaacat
     4681 tccggtactg ccggtggtga aaggcatttc ggtggcgaag aatcacatca cgctgtatat
     4741 catcgccttt gccgttgcca cgctgatgct ctctcttggc ggctacgctg ggtataaata
     4801 tctggtggtc gccgctgcgg tgagcgtctg gtggttaggc atggctctgc gcggctataa
     4861 agttgctgat gacagaatct gggcgcgcaa gctgttcggt ttctctatca tcgctatcac
     4921 tgccctctcg gtgatgatgt ccgttgattt tatggtgccg gactcgcata cgctgctggc
     4981 tgctgtgtgg taacacaacc tctcagttaa aaaggtgcta cggcaccttt tttcttagca
     5041 ttagaatcat atccctctcg aaatatttac taaaaaatcc gcatgtttac cccattcgtt
     5101 tgccgcttta cactagtcgc gaatttaaaa cagaggtggt aatgaacgat tataaaatga
     5161 cgccaggtga gcggcgcgcg acctggggtt tagggaccgt attttcatta cgcatgctgg
     5221 gcatgttcat ggttctgccg gttctgacca cgtacggcat ggctctgcaa ggtgccagcg
     5281 aagcattaat cggtattgcc attggtattt atggtctgac tcaggccgtt tttcagattc
     5341 cgtttggcct gctttcagac cgcattggtc gcaaaccatt aattgtcggt gggctggcgg
     5401 tgtttgccgc cggtagcgtt atcgctgcgc tctctgactc catctgggga attattctgg
     5461 gccgggcgct acaaggctcc ggtgcgattg ccgcagccgt tatggcgctg ctttccgatc
     5521 tcacgcgcga acaaaaccgc accaaagcga tggcgtttat cggcgtgagc tttggcatta
     5581 cctttgccat tgcgatggtg cttggcccga tcatcactca caaacttggg ctgcacgtgc
     5641 tgttctggat gatcgctatt ctggcaacaa ccggcattgc gttgaccatt tgggttgtgc
     5701 ccaacagtag cactcatgta cttaatcgtg agtccggaat ggtaaaaggc agtttcagta
     5761 aagtgctggc ggaaccgcgg ctgctgaaac tcaactttgg cattatgtgt ctgcatattt
     5821 tgctgatgtc gacctttgtt gccctgcccg gacagatggc tgatgcgggg ttcccggcgg
     5881 ctgaacactg gaaggtctac ctggcaacga tgttaatcgc ctttggctcg gtcgtgcctt
     5941 tcattatcta cgctgaagtt aagcgcaaaa tgaagcaagt ctttgtcttc tgcgtcgggt
     6001 tgatcgtggt tgcggaaatt gtgttgtgga acgcacaaac gcagttctgg caactggtgg
     6061 tcggcgtgca gcttttcttt gtggcgttta atttgatgga agcccttctg ccttcactta
     6121 tcagtaaaga atcgccagca ggttacaaag gtacggcgat gggtgtttac tccaccagcc
     6181 agtttcttgg cgtggcgatt ggcggttcgc tgggcggctg gattgacggc atgtttgacg
     6241 gtcagggagt atttctcgct ggcgcaatgc tggccgcagt gtggctggca gtcgccagta
     6301 ccatgaaaga accgccgtat gtcagcagct tgcgcattga aatcccggcg aacattgccg
     6361 caaacgaggc gttaaaagtg cgtttgctgg aaactgaagg catcaaagaa gtgttgattg
     6421 cagaagaaga acattcagct tatgtgaaaa tcgacagcaa agtgacgaat cgctttgagg
     6481 ttgaacaggc aattcgccag gcataaaagc gaggcgaaat tgcctgctgc gctacgctta
     6541 tcaggcctac taagttcctg agttacaaac ctttgtaggc cgaatcaggc gttcatgccg
     6601 catctgacat gaacaaagcg caggttgtcg cgattaatcg cggaagtttt tgaactggaa
     6661 cggctgaccc agatcgccac cacgtaccat cgccatgaca gcctgtaaat catcacgaga
     6721 tttgcccgtc acacggatct catcgccctg aatttgcgcc tgcactttca gtttgctgtc
     6781 tttgatcatt ttgacgattt tcttctgagt cgcgctctca atgccctgtt tcagtttcgc
     6841 ttccacaaac caggttttac cgctatgaac gatattttcc ggtacatcca gtgaactgcc
     6901 ttcaatgccg cgcttcagca acttggcacg cagaatatcc agcaactgat tgacctggaa
     6961 gtcggactcg ctcaacactt tgatggtttt gctggcgtca ttcagctcaa atgaggcttc
     7021 aacgttacgg aagtcaaaac gggactccac ttcgcggctc gcgttatcga ccgcgttacg
     7081 tgcttcctga agatcaactt cagagacaat atcgaaagat ggcatctttt cctctccctt
     7141 catttttgat gcgaagcata atacctgcaa agttaaataa ccaacagtcc tgcaccagga
     7201 cgtatccctt aattgtgcat agtcgcaaca gcatgcacaa ttaagggata gccttattga
     7261 acacccggag tggttgcggg tgaggaggaa caatgaaaat taccgtattg ggatgcggtg
     7321 ccttagggca attatggctt acagcacttt gtaaacaggg tcacgacgtt cagggctggc
     7381 tgcgcgtacc gcaaccttat tgtagtgtga atctggttga gacagatggt tcgatattta
     7441 atgaatcgct gaccgccaac gatcccgatt ttctcgccac cagcgatctg ctcctggtga
     7501 cgctgaaagc atggcaggtt tccgatgccg tcaaaagcct cgcgtccaca ctgcctgtaa
     7561 ccacgccaat actgttaatt cacaacggca tgggcaccat cgaagagttg caaaacattc
     7621 agcagccatt actgatgggc accaccaccc atgccgcccg ccgcgacggc aatgtcatta
     7681 ttcatgtggc aaacggtatc acgcatattg gtccggcacg gcaacaggac ggcgattaca
     7741 gttatctggc ggatattttg caaaccgtat tgcctgacgt cgcgtggcat aacaatattc
     7801 gcgccgagct gtggcgcaag ctggcagtca actgtgtgat taatccactg accgccatct
     7861 ggaattgccc gaatggtgaa ttacgtcatc atccgcaaga aattatgcag atatgcgaag
     7921 aagtcgcggc agtgatcgaa cgcgaagggc atcatacttc agcagaagat ttgcgtgatt
     7981 acgtgatgca ggtgattgat gcaacagcgg aaaatatctc gtcgatgttg caggatatcc
     8041 gcgcgctgcg ccacaccgaa atcgactata tcaatggttt tctcttacgc cgcgcccgcg
     8101 cgcatgggat tgccgtaccg gaaaacaccc gcctgtttga aatggtaaaa agaaaggaga
     8161 gtgaatatga gcgcatcggc actggtttgc ctcgcccctg gtagtgaaga gactgaagcc
     8221 gtcaccacta tcgacctctt ggttcgcggc ggtatcaaag tcaccactgc cagcgtcgcc
     8281 agcgatggta acctggcgat tacctgctcg cgcggcgtga agctgctggc ggatgcgccg
     8341 ctggtcgaag tggctgatgg cgaatatgac gtgattgtgc tgcctggtgg catcaaaggc
     8401 gcagagtgtt ttcgcgatag caccctgctg gttgaaaccg ttaaacagtt ccaccgttcc
     8461 gggcgtatcg tcgcggctat ttgcgccgcg ccagccaccg tgctggtccc gcacgatatc
     8521 ttcccgattg gcaatatgac cggcttccca acgctgaaag acaaaattcc cgccgaacaa
     8581 tggcaggaca agcgcgtcgt ctgggatgca cgggtaaaat tgctgaccag ccaggggccg
     8641 ggtacagcta tcgactttgg tctgaaaatt atcgacctgc tggttgggcg cgagaaagcc
     8701 catgaagtgg catcacaact ggtgatggcg gcagggattt ataattatta cgagtagtgt
     8761 cggatgcggt aagcgtcgca tctgaccaga tgcgacgtta caaccacata ttacgggcga
     8821 tacaccttca cattgttaaa gccctgctcg cgcagataga gcgcctgcag gcggctcatc
     8881 accccgcgct cacaccacaa cagccagttt ttgctctggt cgagatcgcc aaatttggtg
     8941 ctcagtttat agaacggcag agaaaccaca tcaatccctt cgactttcag tggcttatct
     9001 tcctgttcat cgatagaacg gatatcgagg atcacgtcgt tcgggccgaa gccattgacg
     9061 gtttccactt ccaccacttc ctgctcggtc tgctgggcga tttcgcggat atcaacgtta
     9121 ttcgcttcct ccaccacttt atcgagaatg ctgaagtcga acttctcttc ttccgcttca
     9181 atcttcgatt taaccgcttt caccgtcggg cttttggaaa tcacgccgca atattccggc
     9241 atcgtgcggg caaagtcttc ggtgccaatc tggcgagcca ggttgatgat gtgctctttg
     9301 tcgtaagaga tcagcggacg caggatcagc gtatcggaga cgttatcaat caggcgcagg
     9361 ttggtcagcg tctggctgga cacctggccg agcgcttcac cggtaaccag cgcctgtacg
     9421 ccgtaacgtt ctgccacttt ggacgcggca cgcaccatca tacgtttgag gataacgccc
     9481 atctgaccgt cgtcgatttt ctcgagaatt tcaccgacga ccggctcaaa gttaatggcg
     9541 acaaaacgca cgcggtggga gctaccaaaa cggttccaca gataatgcgc cacctgacga
     9601 acaccaattt catgcgccgc gccgccgaga ttaaagaagc agtaatgcac gcggcagccg
     9661 cgacgcatca acatataact ggaaacaccg gagtcgaaac caccggaaat gagcgacaac
     9721 acatcttcct gggtgccgat cgggaaaccg ccaatacctt cgtagcggcc tttaatcagc
     9781 agcagacgat cgtcttccac ttccagatgg acagtcactt ccggattggt cagcttcacg
     9841 cgcgcggatt caatatgctg atttaaaccg ccgccgacgt aacgttccac atcaatcgag
     9901 ctaaaatcat gtttgccgcg gcgcttcacg cgtacgcaga aggttttgcc ttccagctga
     9961 tcgcgatatt gaaccaacgc tttctcgaaa atatcgtgca tgtcggtaaa cggcacgtct
    10021 tcgacttcga gaatatggtg gatacccgga atacgggtca gggcgtcgcg aatagccaga
    10081 cgctggtttt catcttttgc gcgaacttcg atgttatccc agtggcggac gacagcgagc
    10141 gtctcatcat agtgctttaa aacgttacga atgttcccgg taaggatttt tataaagcgc
    10201 aagcgcacag attggctttt gatggtgatt tccgggaaca atttaatgat aaacttcatg
    10261 gcggcaatgg ttcgttggca agccttaagc gacttgtata gggaaaaata cagcagccca
    10321 cacctgcggc tgcatccagg cgcggaagta taccactaac atcgctttgc tgcgcacatc
    10381 accttaccat tgcgcgttat ttgctatttg ccctgagtcc gttaccatga cggggcgaaa
    10441 aatattgaga gtcagacatt cattatgccg aagaaaaatg aggctcccgc cagctttgaa
    10501 aaggcgctga gcgagctgga acagattgta acccgtctgg aaagtggcga cctgccgctg
    10561 gaagaggcgc tgaacgagtt cgaacgcggc gtgcagctgg cacgtcaggg gcaggctaaa
    10621 ttacaacaag ccgaacagcg cgtacaaatt ctgctgtctg acaatgaaga cgcctctcta
    10681 acccctttta caccggacaa tgagtaatgg actttccgca gcaactcgaa gcctgcgtta
    10741 agcaggccaa ccaggcgctg agccgtttta tcgccccact gccctttcag aacactcccg
    10801 tggtcgaaac catgcagtat ggcgcattat taggtggtaa gcgcctgcga cctttcctgg
    10861 tttatgccac tggtcatatg ttcggcgtta gcacaaacac actggacgca cccgctgccg
    10921 ccgtagagtg tatccacgct tactcattaa ttcatgatga tttaccggcg atggatgatg
    10981 acgatctgcg tcgcggtttg ccgacctgcc atgtgaagtt tggcgaagca aacgcgattc
    11041 tcgctggtga cgctttacaa acgctggcgt tctcgattct aagcgatgcc gatatgccgg
    11101 aagtgtcaga tcgcgacaga atttcgatga tttctgagtt ggcgagcgcc agcggtattg
    11161 ccggaatgtg cggtggtcag gcattagatt tagacgctga aggcaaacac gtacctctgg
    11221 acgcgcttga gcgtattcat cgtcataaaa ccggcgcatt gattcgcgcc gccgttcgcc
    11281 ttggtgcatt aagcgccgga gataaaggac gtcgtgctct gccagtactc gacaagtacg
    11341 cagagagcat cggccttgcc ttccaggttc aggatgacat cctggatgtg gtgggagata
    11401 ctgcaacgtt gggaaaacgc cagggtgccg accagcaact tggtaaaagt acctaccctg
    11461 cacttctggg tcttgagcaa gcccggaaga aagcccggga tctgatcgac gatgcccgtc
    11521 agtcgctgaa acaactgact gaacagtcat tcgatacctc ggcactggaa gcgctagcgg
    11581 actacatcat ccagcgtaat aaataaacaa taagtattaa taggcccctg atgagttttg
    11641 atattgccaa atacccgacc ctggcactgg tcgactccac ccaggagtta cgactgttgc
    11701 cgaaagagag tttaccgaaa ctctgcgacg aactgcgccg ctatttactc gacagcgtga
    11761 gccgttccag cgggcacttc gcctccgggc tgggcacggt cgaactgacc gtggcgctgc
    11821 actatgtcta caataccccg tttgaccagt taatctggga tgtggggcat caggcttatc
    11881 cgcataaaat tttgaccggg cgccgcgaca aaatcggcac catccgtcag aaaggtggcc
    11941 tgcacccgtt cccgtggcgc ggcgaaagcg aatatgacgt attaagcgtc gggcattcat
    12001 caacctccat cagtgccgga attggtattg cggttgctgc cgagaaagaa ggcaaaaatc
    12061 gccgcaccgt ctgtgtcatt ggcgatggcg cgattactgc tggcatggcg tttgaagcga
    12121 tgaatcacgc gggcgatatc cgtcctgata tgctggtggt tctcaacgac aatgaaatgt
    12181 cgatttccga aaatgttggc gcgctcaaca accatctggc gcagctgctt tccggtaagc
    12241 tttactcttc actgcgcgaa ggcggaaaaa aagttttctc tggcgtgcca cccattaaag
    12301 agctgctcaa acgtaccgaa gaacatatta aaggcatggt agtgcctggc acgttgtttg
    12361 aagagctggg ctttaactac atcggcccgg tggacggtca cgatgtgctg gggcttatca
    12421 ccacgcttaa gaacatgcgc gacctgaaag gcccgcagtt cctgcatatc atgaccaaaa
    12481 aaggtcgtgg ttatgaaccg gcagaaaaag acccaatcac cttccacgcc gtgcctaaat
    12541 ttgatccctc cagcggttgt ctgccgaaaa gtagcggcgg tttaccaagc tattcaaaaa
    12601 tctttggcga ctggttgtgc gaaaccgcag cgaaagataa caagctgatg gcgattactc
    12661 cggcgatgcg tgaaggttcc ggcatggtcg agttttcacg taaattcccg gatcgctact
    12721 ttgacgtggc aattgccgag caacacgcag taacctttgc tgcgggtctg gcgattggag
    12781 gctacaaacc cattgtcgcg atctactcca ccttcctgca acgcgcctat gatcaggtgc
    12841 tgcatgacgt ggcgattcaa aagcttccgg tcctgttcgc catcgaccgt gcgggcattg
    12901 ttggcgctga cggtcaaacc caccagggcg cttttgatct ctcttacctg cgctgcatac
    12961 cggaaatggt cattatgacc ccgagcgatg aaaacgaatg tcgccagatg ctctataccg
    13021 gctatcacta taacgatggc ccgtccgcgg tgcgctaccc gcgcggcaac gcagttggcg
    13081 tggaactgac gccactggaa aaactgccaa ttggcaaagg cattgtgaag cgtcgtggtg
    13141 agaaactggc gatccttaac tttggtacgc tgatgccaga agcggcgaaa gtcgctgaat
    13201 cgctgaacgc tacgctggtc gatatgcgtt tcgtgaaacc gcttgatgaa gcgttaattc
    13261 tggaaatggc cgccagccat gaagcgctgg ttaccgtaga agaaaacgcc attatgggcg
    13321 gtgcaggcag cggcgtgaac gaagtgttga tggcccatcg taaaccagta cccgtgctga
    13381 acattggcct gccggacttc tttattccac aaggaactca ggaagaaatg cgcgccgaac
    13441 tcggcctcga tgccaccggt atggaagcca aaatcaaagc ctggctggca taatccctac
    13501 tccactcctg ctatgcttaa gaaattattc atggcaggag tggcagcatg caatacaacc
    13561 ccttaggaaa aaccgacctt cgcgtttccc gactttgcct cggatgtatg acctttggcg
    13621 agccagatcg cggtaatcac gcatggacac tgccggaaga gagcagccgt cccatcatta
    13681 aacgtgcgct ggaaggcggc ataaatttct ttgataccgc caatagctat tccgatggca
    13741 gcagcgaaga gatcgtcggt cgcgcactgc gggatttcgc ccgtcgtgaa gacgtggtcg
    13801 ttgcgaccaa agtgttccat cgcgttggtg atttacctga aggattatcc cgtgcacaaa
    13861 ttttgcgctc tatcgacgac agcctgcgcc gtctcggcat ggattatgtc gatatcctgc
    13921 aaattcatcg ctgggattac aacacgccga tcgaagagac gctggaagcc ctgaacgacg
    13981 tggtaaaagt cgggaaagcg cgttatatcg gcgcgtcatc catgcacgct tcgcagtttg
    14041 ctcaagcact ggaactacaa aaacagcacg gctgggcgca gtttgtcagt atgcaggatc
    14101 actacaatct gatttatcgt gaagaagagc gcgagatgct gccgctgtgt tatcaggaag
    14161 gcgtggcggt gattccatgg agcccgctgg cgcggggccg tctgacgcgt ccttggggag
    14221 aaaccaccgc acggctggtg tcggatgaag tggggaaaaa tctctaccaa gaaagcgatg
    14281 aaaatgacgc acaaattgca gaacgcttaa cgggcgtcag tgaagaactt ggtgcaacgc
    14341 gagcacaagt tgcgctggcc tggttgttga gtaaaccggg cattgccgca ccgattatcg
    14401 gaacttctcg ggaggaacag cttgatgaac tgctaaacgc ggtggatatc accttgaagc
    14461 cggaacaaat cgccgagctg gaaacgccgt ataaaccgca tccggtcgta ggatttaaat
    14521 aggtatacag gcctgatgag acgtgacaag cgtcacatca ggcatcggtg cacaactacg
    14581 acagaatacc cagcggccag tgatgtccaa taaagtacag gatgactgcg gagatcaccc
    14641 cggcgacaat atcgtcaatc atgatcccca tgccgccatg tacattgcga tcaaaccagc
    14701 ggatcggcca cggcttccac atatccagaa tacggaaaat cacaaatccg gcggtaaccc
    14761 actgccagtc attggtcggc agcgccatga gcgtaatcca cataccaata aattcgtccc
    14821 agacaatact gccgtgatcg tgcacgccca tgtctttcgc cgtttgatga caaagataga
    14881 cgccgataca gatccccagc atcaccacca gcgaatagag ctgccagggc aaaaaagtca
    14941 tcagatacca gaagggaatc gccgccagcg accccatcgt gccaggaacg atcgggctta
    15001 atccacttcc gaatccgaca gcaagcagat gccacggatt actcattttc aggcgacttt
    15061 tcgcgacatc tttatggcgt ggcaaaatgg tcatatcctt tccagtctaa cgtgacaggt
    15121 tcgccgtcac gaataaaaca aagcccttcg atatcggcgg tcatttgccc gatacaggta
    15181 aacggtacgc ccaggtgtcc gagagccaca tccagcgcgc cacggttcag ttccggcacc
    15241 gtaaaacaca actcgtaatc ttcgccgcca gagagcgccc agcgcagcgc ctgttccggt
    15301 tcaacatggc gagaaagcgc atcagaaaac ggcagcaatg ccaggtcaat acgtgcgccg
    15361 cagtcgctgg ctttcacgat atgcccgaga tcggaaatca gaccgtcaga gagatcgata
    15421 gctgaatttg ccagatcgcg cagtgcctgc ccctgtaaaa tacgtggcga cggacggaga
    15481 tgacgtttga tcaagtagtc cgcatcttta gcatcggcaa cctgcaaacg gttttgcaaa
    15541 atcgccagcc cggcagcgct atcgcccggt gtaccggtca catagatcca gtcacccggt
    15601 ttcgccccag agcgcgttaa ggctcgtccc atcggaacaa agccgtggat acccaacgtc
    15661 attgataatg gcccacgcgt ggtatcgccg ccaataagtt gcatatcgta ataattgaga
    15721 agatcaaaca agctgtcgct gaaggactca agccacgctt cgtctacgtc cggtaaggtt
    15781 aatgccagcg tcagccaggc cggatcggcc cccatcgctg ccagatcgct taggttcacc
    15841 gccagtgctt tataagccag atcagcagga tcgatatcag ggaggaagtg gttgcccgcc
    15901 accagcgtat cagtgctgat cgccagggtc tgtttctcgg ggatattgag aagagcgcaa
    15961 tcgtcgccga tgcccagttc gacatcaaga cgagaacttc ttacacggtc aaaataacgg
    16021 gcaatcaggg agaactcgcc acatgccata cgttacgcct cagcagataa aaaagaaaag
    16081 gccggcgatc atggaacggt cttccgtgaa tctaccggct tggatattac tttttgttag
    16141 ggcgaatcac aggtgctgct ttatcgagta cgccgttgac gaacttatgg ctgtcttctg
    16201 cgccgaacga tttcgccagt tcgatcgctt cgttaatggc cactttgtat ggcacatcgc
    16261 tacgtttaga cagctcgtac agcgcaatgc gcagtactgc tttttctacc tggcccagtt
    16321 cttccagcag acgggacagg tacggcttca tcagtccgtc gaggtatgcg gtgttagtcg
    16381 ccaccccggc cagcagctca cggaagtaca ggacgtcaac gtcttttaca tcctgttcag
    16441 ccaggaactg gtattcaaca tcagcgatgt cgttctggga caactgccag gagtagagcg
    16501 cctggacggc acactcacga gcgcggcgac gagcagcagg tttcacggat ttccccttac
    16561 taatttcagg ccttgatggc tttcaataca ttaatcattt caagcgcggt cagtgcagct
    16621 tctgcacctt tgttgccagc tttggtgcca gcacgttcga tcgcttgttc aatgctttca
    16681 gtggtcagaa caccaaaagc aaccggaatt tcgctgtcct gggcaacatg cgccaggccg
    16741 ttgcttgcac caccagcgac atattcaaag tgggcagtgc caccacgaat aaccgtaccc
    16801 agcgcaatca ccgcgtcgta tttaccggtt ttagccagtg cacccgccgc cagcggcagc
    16861 tcataggcac caggcaccca aacaacggta atgttttcat cttttacctg accgatacgt
    16921 ttcagtgcgt caattgcacc ttccagcagg ctgtcattga taaagttgtt gaaacgcgcg
    16981 atggtgatgg cgacgcgagc gtccggggta gcaacgttag cttcaataat gttcattttc
    17041 ttccttcggg ttcgagtatg gccccgcagg ggggcggatt ttagcataat atttcgtgcg
    17101 ctgcttccct ttcgagccgg gagatcatgc acccactaaa tgcaggcaaa catccgggcc
    17161 tacatgacgt atctctttga atttaaattg gggagcgtcg gctaatttct caagccctgg
    17221 cagcgtgcat aatccacggg cgtcgttgcc taatagttta ggtgcgatat agacaatcag
    17281 ctcatcgact aaacccgcct gtagcaatgc gccagcgagc gttggccccg cttccaccca
    17341 gatgctgtta atctgctgtt tacccagttg catcattagt acaaccagat ccaggtgacc
    17401 tttatgctct ggaatcagca aggtacgcac cgtttccggc cactcacgag aatcttcctg
    17461 cgtacgcgcg aaccaggttt cgccgggctg ctgcacaatg cgatgttccg gcgtcacgcg
    17521 attttggcta tcaatcacaa tacgtactgg ctgacggaga ttttgttgcg ggtagagccc
    17581 ctgagtttgt tcatccagtt cagaccaacg cactgttaag gctggatcat ccgccagcac
    17641 cgtggcgctg ctggttaaaa tggcatgact ttgcgcgcgc agtcgttgta cgtcgcgtcg
    17701 cgcctgaggc gaagtgatcc actggctttc gccactcgcc atcgccgtgc gaccatcaag
    17761 cgatgcgcca agttttaact gaatataagg aaagccggtg cgcatccgct tgagaaagcc
    17821 tttattcaat tgctcagctt cactcatcat cagcccgtgg ctgacgtcaa tgccagcctg
    17881 ttgcagacgg taaagtccac gtccagcaac ctgcggattg gggtcttgca ttgcagcaac
    17941 cacgcgcgcc acgcccgcag caattaatgc atcacaacac ggtggcgtac gaccatgatg
    18001 gctacagggt tcgagtgtga catacgccgt tgcgcctttg gctttttcgc cggccatgcg
    18061 caacgcgtgt acctcggcat gcggttcacc cgctcgttga tggtaacctt caccgacaat
    18121 ttcgccatct ttgacaatga cgcacccgac attcgggttg ggatgcgtgg taaaacgtcc
    18181 tcgttgcgcc agctttagcg cccgcgccat gtaatactcg tcctgcacgg cttagtcctc
    18241 caggcgcgcg atctcttcgc caaattcttt gatatcttcg aaactgcggt agacagaggc
    18301 aaaacggata taggcgactt tatcgagctt tttcaattgc tccatcacca gattgccaat
    18361 catcttgctc ggcacttcgc gctcaccggt ggcgcgcagc tgcgatttta tatgattgat
    18421 tgccatttcg acgtcatcgg aactcaccgg acgtttttcc agcgcccgca gcattccgct
    18481 acgtaatttc tcttcattaa acggttcacg cacgtcgttg ctttttacaa cacgcggcat
    18541 aaccagctcc gccacttcaa aggtggtgaa acgttcatta cacaccagac actgccgacg
    18601 gcggcgtacg gatgaaccct cgcccacgag acgagagtca attaccttag tgtccacggc
    18661 gaaacagaat gggcaatgca tacggcgtcc tgaccaggtg gttaacagaa atctatttta
    18721 ccctgaactg acgatacaac aaaggcgcaa cgcttttgag ataaaggatc gatgctttca
    18781 ctgcttttac cctctaccat taggctaatt gcgacgagaa agggacgacc tatgaatact
    18841 aacgtttttc gactgctcct gctgggaagc ctgttcagcc ttagcgcctg tgtgcagcaa
    18901 agtgaagtgc gacagatgaa acacagcgtc agcacgctga accaggagat gacgcaactc
    18961 aataaagaaa cggtcaaaat cactcagcaa aacaagctga atgcaaaatc cagcagtggg
    19021 gtttaccttc tgcctggagc aaaaacacca gcaagactgg aaagccagat cggtacttta
    19081 cgtatgtcgc tggtgaatat tacgcctgac acagatggca ctaccctgac gctacgtatt
    19141 cagggcgaat ccaatgaccc tttacccgca ttcagcggaa ccgttgaata tgggcaaatt
    19201 cagggaacaa tagacaactt tcaggaaatc aatgtgcaga atcaattgat taacgcccct
    19261 gccagcgtcc tcgcccccag cgatgttgat attccgttac agttaaaggg tatttctgtg
    19321 gatcagttag gctttgtacg tatccacgac attcaacctg ttatgcagta aacgtattgc
    19381 ggggcgatat tgtgcgtccc gcaacatctt tctccgtcat tttgttactc cgcttacatc
    19441 acctggattg atagtaaaag tttgcaacaa gggtgaaagt cagtacaatc cccgcccgaa
    19501 tgtgtgtaaa cgtgaacgca atcgattacg taaatgatag aactgtgaaa cgaaacatat
    19561 ttttgtgagc aatgattttt ataataggct cctctgtata cgaaatattt agaaacgcaa
    19621 tttgcgcctt tttcactccc gcaagggatt ttcaaacagt ggcatacata tgaaaaaaac
    19681 attactggca gccggtgcgg tactggcgct ctcttcgtct tttactgtca acgcagctga
    19741 aaacgacaaa ccgcagtatc tttccgactg gtggcaccag agcgttaacg ttgtcggaag
    19801 ctatcacacc cgtttcggac cgcagctccg caacgatacc taccttgagt acgaagcatt
    19861 cgctaaaaaa gactggttcg acttctatgg ttatgcggat gcgccggtat tcttcggcgg
    19921 taactccgat gcaaaaggta tctggaacca cggttctccg ctgtttatgg aaatcgaacc
    19981 gcgtttctcc attgacaagc tgaccaatac tgaccttagc ttcggtccgt tcaaagagtg
    20041 gtacttcgcg aacaactaca tttacgacat gggtcgtaat aaagatggcc gccagagcac
    20101 ctggtacatg ggtctgggta ccgacatcga tactggtctg ccgatgagcc tgtccatgaa
    20161 cgtctatgcg aaataccagt ggcagaacta cggcgcagcg aacgaaaacg agtgggacgg
    20221 ttaccgtttc aaagtgaagt actttgtgcc gattaccgat ctgtggggcg gccagctgag
    20281 ctacatcggc ttcaccaact tcgactgggg ttccgacctg ggcgatgaca gcggttatgc
    20341 gaacaacggt attaaaacac gtaccaataa ctctattgcg tccagccata ttctggcgct
    20401 gaattacgat cactggcact actctgtcgt tgcccgttac tggcataacg gtggtcagtg
    20461 gaatgacgat gcagaactga acttcggcaa cggcaacttc aacgttcgct ctaccggctg
    20521 gggtggttat ctggtagtag gttacaactt ctaattgtga gaatgccgga tttcatatcc
    20581 ggcatttttt atcacttctt cttgttcatc atcgctttca aatcagcgaa tgggttgtac
    20641 ttcgcttcac cgacatcttt ctgcgcatct tcccctgcga taacggtcgt accatactga
    20701 tccgcttcgg tgtatttcga atgctcatga tcgtggcaat agagacaaag taattcccag
    20761 ttactgccat cttccgggtt attagtatgg tcgtgatcaa tgtggtgaac ggtaagttca
    20821 cgcaggttgg aataaacaaa ctcgcgggaa cagcgaccgc agacccacgg atagattttt
    20881 aatgcttttt cgcgatagcc gctttctaac cgcgcatagt tttttgggat gatagccatg
    20941 aaaaagttac ctggatgaaa tgtagggtta cgcgcagttt cccaagatgc ggggattttc
    21001 gcaatcacct tacatcaaaa aaaatcccga tcttctgacc gggattttca acatcagctg
    21061 aaacttgatt aaggcagaat tgacggctga tccgcccctt ctttttccac tttctgctgc
    21121 aacatgtgtt cgcgcttcat acccagtttc agagccagcg cagatgccac atagatggaa
    21181 gatgcagtac cgatggaaac accgataagc atggtcagcg agaagccttc cagtaccgga
    21241 ccaccgaaga ggtacagcat caggataacc atcaaggtag taccggatgt gatcaaggta
    21301 cggtgcagcg tctgggtcaa ggacacgtta aagatttcgt aaggcgtacc gcgacggatc
    21361 ttgcggaagt tttcacgaat acggtccgat accacgatac tgtcgttaag cgagtaaccg
    21421 ataaccgaca tcaacgatgc cacaatggtc aggtcaatct cgatatggaa taacgacaaa
    21481 atacccagcg taatgataac gtcgtgcgcc agcgcaataa caacccctgc cgccagtcgc
    21541 cactcaaagc ggaaaccaac gtacacgagg atagacagca gcgccgccat caacgccatc
    21601 gcaccggttt gcgcaaggtc tgcccccacg ctcggaccga cgaactcaat acgcttcact
    21661 gccgcattct gattggtgga ttcgttaatc accttcagaa cctggctgcc cagcacctga
    21721 ccaccggttt cgccttcagc aggcggcata cggaccatga tgtcgtggct gctaccaaag
    21781 ttttgcagca tcggctcttc aaaaccggct ttttgcaatg catcacgcat tacgtcaatt
    21841 tcagccggtt tttcgagcgt aatttcaata accgtaccac cggtgaaatc cagcccccag
    21901 ttaaagccgc gcacgcccat aataacgata gcggcgatta acagcagacc agagatgccg
    21961 aaagcccagt agtcccagcg cataaagtca tagactttac ggccgtggtt tagttgttca
    22021 acagtatatt cctgtgccac atcgcactcc tcagattgac agcttcttga cgcgcttgcc
    22081 gccatatagc aggtttacga tggcacgcgt accgacaatc gcggtaaaca tcgacgtcgc
    22141 cacaccgata ccggtagtaa tcgcgaaccc tttaattgcc ccggtaccca ctgcgtacag
    22201 gatgatgact ttaatcagcg tggtgatgtt cgcatcgaag atggaactga atgcgccacg
    22261 ataaccttca tcaattgcct gttgaacagt acgtccgttg ctcaactctt ctttaatacg
    22321 ttcgttgatc agtacgttcg catcgaccgc taccgcaagg gttaagacga tacccgcgat
    22381 acctggcata ctcagcgttg cgcctggcaa cagcgacata ataccgacaa tcaagatcaa
    22441 gttggcaatc agagcactgg tcgcaatcag accaaacttc ttatagaaga tgatcatgaa
    22501 cagaatagaa accagcagac cggcaaggca agcctccagc ccctgctcaa tgttctgcat
    22561 acccagggtt gggccaatgg tacgttcttc aacaatctga atcggcgcga tcaacgcacc
    22621 ggcacgcagc agcagtgaaa gctgacgcgc ttcgcttggg ttattgatgc cagtaatacg
    22681 gaagctgtta cccagacgag actggatgtt ggcgatatta atcacctctt cctgtttcac
    22741 cagaaccgca cgaccgtttg catctttctt accgctgtct ttgtactcca caaacagggt
    22801 tgccatcggt ttgccgatgt tgtccttggt gaagttagac atgatgttac cacccgcgct
    22861 atcgagagag atgttaacct gcggctggtt gtattcgtcc tggctggaag tggagtcggt
    22921 gatatggtca ccggtcagaa ttacgcgttt gtacagcaca accggctgac cttcgcgggt
    22981 ctgtttcact tcagagtcgc ccggtacgcg accggatgcc gcagcggcct gatcaacgtt
    23041 ggtgtttacc agacggaatt ccagcgttgc cgtcgcaccc agaatctctt tcgcacgcgc
    23101 agtgtcctga atacctggca gttcaacaac gatacggtca gcaccctgac gctgaaccac
    23161 cggctccgcc acgccaagtt ggtttacacg gttacgcagg atattgatgt tctgctgcac
    23221 cgcatattca cgcgcttcac tcagacgagc atcgctcatt accgcacgca gctggttgct
    23281 gccctggctg ctaatcacca ggtccggatg gcgcttgctc agatacgcaa tggcttcatc
    23341 acgagcttta gcatcgcgga aagtgatgct caggccgtag ttgttttctt tacgaacggt
    23401 ggtatacggg atgccctttt cacgcaggtc gctgcgcagg ctatcgatat tttgttcctg
    23461 gagtttgcca agcgcggtgt ccatatccac ttccatcagg aagtgaacgc cgccacgcag
    23521 gtcaaggccg agcttcatcg gctcagcatg aatagctgcc agccagcgcg gcgttgccgg
    23581 ggcaagatta agcgccacga cgtatttgtc acccataacg cccattaatg cttcacgagc
    23641 gcgcagctgg gtgtcagtgg agtcgaagcg cgcaagaata gcgccctctt ccagtgccac
    23701 agacttagca gttatttttt cttcttgtaa cgttttctgg acctggatca gcgtttgctc
    23761 actggcggcg actccgcgcg caccagtgat ctgaacagcc ggatcctcac caaacaggtt
    23821 gggaagcgca tacagcagac cgatgacaat caccacgatc agcatgacgt acttccacaa
    23881 aggataacgg tttaacacgg caattccctt agggaaaaat tttaattaca gcgccttcat
    23941 ggtgcctttc ggcagaacgg cagctacgaa gtcacgttta ataactactt cagtggtgtc
    24001 attcagtgcg atagcaatgt agccgttttc cgctactttg gttacgcgac cgaccaggcc
    24061 accgttcgtc agcacttcat cacctttagc gatggagtcc atcagctttt tgtgttcttt
    24121 ggtgcgcttc tgctgtggac gcaggatcat gaaatagaaa atcagaccga ataccaccag
    24181 catcaaaatc aaagacatcg ggctaccttg cgccggtgca cccgttgccg ctaccgcatc
    24241 agaaataaaa aagctcatta aatttccctc attattaata ttaatcaacg ttcaaaggtg
    24301 gtacttctcg cccctgacgc tggtaaaaat cagttacgaa gctctctaat ttaccctctt
    24361 caatagcctt gcgtaaaccc gccatcaaac gctggtagta acgaaggtta tgaatggtgt
    24421 tgagtcgcgc gcctaatatt tcgttgcaac ggtcgagatg atgcaagtaa gcgcgtgaat
    24481 aattgcgaca ggtgtagcaa tcacactcag gatcgagtgg gccagtatcg ctcttatact
    24541 tcgcattgcg gattttcacc acgccatcgg tcacgaacaa atgaccattt cgggcgttgc
    24601 gggttggcat tacgcagtca aacatatcga taccgcgacg tacgccctca accaggtctt
    24661 ctggtttacc aacgcccatc aggtaacgcg gtttgtctgc cggaatttgc gggcatacat
    24721 gctccagaat gcggtgcata tctgctttcg gctcacccac agccagaccg ccgacagcgt
    24781 agccatcaaa accgatatct accagacctt taacagaaat atcacgtaaa tcttcgtaaa
    24841 cactgccctg aatgatacca aacagcgcat ttttgtttcc gagactgtca aaacgctcac
    24901 ggctacgctt cgcccaacgc agagacatct ccatggagcg ttttgcataa tcccagtcag
    24961 caggatacgg cgtacactca tcaaagatca tgacgatatc cgaaccaaga tcgtactgaa
    25021 tctccatcga cttttccggg tcgaggaaaa tcgggtcgcc gttgatcggg ttacggaagt
    25081 gaacaccctg ttcggtgatt ttacgaatat caccaaggct gaaaacctgg aagccgccgg
    25141 agtcggtaag gattggtcct ttccactgca taaaatcgtg cagatcgccg tgcagtttca
    25201 tgatttcctg gcccgggcgc agccacaggt ggaaggtgtt gccgaggata atttgcgcgc
    25261 cagtggcttc aacttcttcc ggcgtcatcc cttttacggt gccgtaagtg ccaacaggca
    25321 taaaacaagg cgtttccact acgccacgat caaagaccag gcggccacgg cgtgcgcgac
    25381 cgtcggtggt gtccagttca aatttcattt tttctccact acgtcagaaa aacagtccaa
    25441 cgtttaaacc agcgccgcgg aattactccc caacgcgctc attaattgcc tgcggattgt
    25501 acgtgataaa catcgcatca ccgtaactaa aaaagcgata tttctcttct accgccgctt
    25561 tataggcatt catggtgtgt tgataaccag caaatgccga aaccagcata atcagcgtcg
    25621 attccggaag gtggaagttc gtcaccagcg catcgaccac tttgtactgg aagcccggat
    25681 agataaagat ttgggtatcg tcaaagaacg gttcaatgag atcgtttttc gctgcctgag
    25741 ccgcactttc cagcgaacgt actgaggtgg tgccaaccgc aatcacccgg ttaccgcgcg
    25801 ctttcgccgc cagtaccgcg tctaccacat cctgcggcac ttcagcgtat tccgagtgca
    25861 tgatgtgatc ttcaatggtg tcgacgcgca ccggctggaa ggtgcccgca ccaacgtgca
    25921 acgtcacaaa cgccatctcc acgcctttgg cgcgtaattt ttccagcaaa ggttcgtcaa
    25981 agtgcagtcc ggcggtcggt gctgctaccg cgccgggttt ttcgctataa accgtttgat
    26041 aaagttcgcg gtctgcatct tcgtccggac ggtcgatgta cggcggcagc ggcatatggc
    26101 cgatgctgtt gagaatatcc agcaccgaac gttgatcatt aaattcgact tcaaacagtg
    26161 cgccgtggcg cgcggtcatt gttgcgttaa tactttcgtc atcgcccagc agcagttctg
    26221 cgccaggttt tggcgctttc gaggcgcgaa tatgcgcaag aatgcgtttg tcgtcgagca
    26281 tccgctcaac cagcacttca atcttgccgc cgctggcttt acgcccaaac agacgcgccg
    26341 ggatcacgcg ggtattatta aaaaccagaa gatcgccggg gttgagctta tcgagtaaat
    26401 cggtgaaagt accgtgcgtc agcgcgcccg tcggcccgtc cagcgacagt aaacgacagc
    26461 tactgcgttc aggcatggga tagtgggcaa tcagggattc gggcaattca aaagagaaat
    26521 cggtaacgcg catgacactg actcagacta aaataagagg cgggtagtct agtgccgggg
    26581 cgctctccct gcaaccttta ccccccttta gatagaatat aatcgatgaa ttttttagct
    26641 cacctgcatt tagcccatct cgcggaaagc tcgctttccg gcaatttact ggctgatttc
    26701 gtacgcggaa atcctgaaga aagttttccg cccgacgtcg tggctggcat tcatatgcat
    26761 cggcgaatcg acgtattaac cgacaatctg ccagaagtcc gcgaagcgcg ggagtggttt
    26821 cgtaatgaaa cgcgccgtgt cgcgcctatt acgctggatg tgatgtggga tcactttctg
    26881 tcccgccact ggtcgcagct gtcgcccgat tttccgctac aggaatttac ctgttatgcc
    26941 cgggagcaag tgatgactat tcttccagac tcaccgccac gctttatcaa tctgaacaac
    27001 tacttatggt cagagcagtg gttggtgcgc tatcgcgata tggatttcat ccagagcgtg
    27061 ttaaacggca tggcaagccg tcgcccacgt ctggatgccc tgcgtgactc ctggtacgat
    27121 ttagacgccc attacgatgc tctcgaaacc cgcttctggc agttttatcc gcggatgatg
    27181 gagcaggcgt cacgcaaggc gttataagac gttaattcat ccacaccgta gccgaaatgg
    27241 caggcaaagc cagaatcccg tccgtcagtt gcccgtgacc ttctttgcgt tgccattgcg
    27301 cgacattaag caacggcgac gcgggtagca ccacttcaca ggcttcgcca cggttgattg
    27361 ccaccagtac acgctgctga ttcagcacgc ggacaaatac caccacgtta tcttccgcat
    27421 acagcacctg acagccgcca cgacgcaacg cctgactttt cttacgcagc gcaatcattc
    27481 gctggtacag cgcgaataac gccgaatcct gcttttccac ctgccagggg aacggcttac
    27541 ggcaaaacgg atcgtttttg ccatccagtc ccacttcatc accgtaataa atgcacggta
    27601 caccaggcca ggtgaacagc cagaccaccg ccaggggcag gcgcgcaaca tcccgaccga
    27661 gcagcgtttt aaatcgcgca gtatcgtggc tgtcgagctg attaaacata cgtaattgtt
    27721 gttgatgaga aagccctgcg cggtaattat ccatccaggc catacaggtt tgggcgtcaa
    27781 tttgctgcgg atcgtaagag atatcggtgt tggcaagaaa tccccacaac gggaatgtga
    27841 agccgcgata gttcatggcg gcatcttcca catcggcctg taaccattgc cgtgcatcgc
    27901 caaaatgttc gccgacaata tacgcatccg gctgggtttc tttcgccgct tcggtgatcc
    27961 cggcaacgtg ctgcaaatta ttgcgcgccc cacccgcttc ccccagcata tgcaccacat
    28021 ccagccgcca gccatccata ttccacggcg ctttcagcca gtggcggaca atactgtctt
    28081 ccccgcgata aatttcattc accagacttt ccgactgata atccagcttc ggcaagctgg
    28141 catagcccag ccagtcgagc gccgtgccat catcactaaa cgagtaccag tcacgccagg
    28201 gtgattcggg gttgtgacaa gctccccccg tgccacgatt gtgcctgtca aaccaggcat
    28261 gggaatcgcc actgtggtta aacacgccgt ccagcaccag tcgtattccc agctgctgcg
    28321 tattgtgtcg caaacgcagc aacgccccat caccgccaaa ctgcggatcg acatggcgat
    28381 aatcctcggt atcgtattta tgcacgctgg gagctttaaa caccggattg agatacagcg
    28441 ccgtcacgcc aagttttttc agatacggca gtttttcact tatcccgtcc agatcgccgc
    28501 catagaacgt tgatccgcca gcctgcgccg tgaccggttc atcccagtca cgcaatatga
    28561 tctcttgtcc ggctgcgtga tggtaataga catgatcctg ttcagcttca cgaggaagat
    28621 tacgggcgaa acgatcaggg aagatctgat aaaaaatctg atccgcagcc cattgtgggc
    28681 cgatatccgg tacatcgatg gcaaactgct cctgtcgtgc cggcggcatt cggctgaagc
    28741 cttgcggtgt aaaccaacgc tggcgatcgt gccacagcaa tttgaaacta taacgccgcc
    28801 gaggttgtcc gcaggagagc tcgatcgccg cccgccatgc ggtgacgcca ggctgcggct
    28861 gactgcgctg cttatgcatc ggtactgaca tttcttcgtt atcgtgttct gtacgcagca
    28921 taatgcgctg cggtgggtct tcgcccgtca gccacagtgt aatgagcagt tgatctttgc
    28981 tttgtttaac aaatgggggc accggcagat gccatgcatt taacatcata attcccctgt
    29041 gatgaaaatc tgatcacctt gccatagcct aatgcaggat cactcatcat ctgaaggata
    29101 tttgggggag gaggacgggg gcggataact ttcggaatag ccggatcagg atttcctcct
    29161 gaaccggctt acagaacgca aggattactg gttttcagcc agctgacgat cgtggcggcg
    29221 cttaaacatc cagccaatca acagcacaac aatccacgcg aaaccaacat acagcgagat
    29281 acgcgtatca gggtgataac caatcaaccc gataataaag agcaggaaaa tcaaaccgcc
    29341 gatggtcgtt gctaccccac ccggcacttt aaatttcagc gccttaactt cttctggcgg
    29401 caaacggcga cggaaggcga tttgcgacag caggatcata atccacaccc acaccgtggc
    29461 gaaggttgcc agcgaagcga tcaccaggaa gacgttttcc ggcatgatgt agttcagata
    29521 caccgcaaac agcagcgcgg tagtcatcac cagcaccgta acccacggaa taccgcgacg
    29581 cgaggttttg ctgaaaattt ttggcgcgct gccctgctct gccataccgt ggagcatacg
    29641 gcctacacca aatacgtcac tgttaattgc cgacagcgaa gcggtcagca caacaaagtt
    29701 aagaatgctg gcggcaaagg taatgcccat atgctggaac gtcagcacaa acgggctacc
    29761 ggcagtgcca acctgattcc acgggtagat agacataatg acgaacagcg taccgacgta
    29821 gaacaccaga atacgcatcg gcacggagtt aatcgcacgc ggaatcgatt tctcaggatc
    29881 tttcgcttca ccggcggtaa tcccgataat ttcgatccca ccgtaagcaa acatcaccat
    29941 ttgcaacgac atcaccatac caagccagcc gttactgaag aagccgccgt tgctccacag
    30001 gttatgaata ccggtcggtt gcccgccgtt gccaatcccc cagatgatga tgccgaaacc
    30061 ggcgacaatc atgatgatga tagtggcgac tttaaagaac gagaaccaga actccagctc
    30121 accgaatacc ttcacgctca tcaggtttac ggcgcaaatg atcagcacca cactcagtac
    30181 ccaaatccag tgcggcaccg tcgggaacca gacacccata tagataccaa aagcggtcac
    30241 atcggcgatg gcgacaataa ggatttcaaa gcagtaggtc cagccggtaa tgtaacctgc
    30301 cagcgggccg aggttttcct gcgcataacg cgagaaagag ctggctgccg ggttatgtac
    30361 cgacatttcc cccagcgcac gcataatgat atacgccgcg ataccaccga taatataggc
    30421 caacaacacg cttggaccgg ccattttgat ggcatctgcc gaaccgtaaa acagcccggt
    30481 gccaattgct gaacccagtg ccataaagcg tatgtgtcgg gtacttagcc cacgctttag
    30541 cttgttctta ctttccatca attcctaacc catcaacaca ataaaaaacc acggggcatc
    30601 gcagccccgt ggttaaaaca aatgttcagt gatttagtga gcgctggagg tcacctgacg
    30661 acctgccgcg cgatcccaga taatggccag aaccaccatc accactgttg gcattaacca
    30721 cgccagacct tgttcggcca gcggtaaacg ctgcgcccag gacggtaaga tatcgctgaa
    30781 tgcagatgcc ttgatcccgt cgagaatacc aaaaagcagg ctgataaaca tcggcggagc
    30841 aatcacgcgg gacgaattat gccaccatga gcgtgtaaaa cttaatacaa ccagtgcgat
    30901 acacggcgga taaatggcgg tcagcaccgg tacagagatc tgaatcagct ggctcaagcc
    30961 gaggttagac accaccatcg agaagccgcc gaggataaac accagcgtac gataagagag
    31021 cggtacgtac tgggcgaaga attctgcaca agcacaggtc aggccaaccg ccgtgaccag
    31081 gcaggcgatg aagattaacg ccgccagcag gaagctaccg ccgccgccaa aggtatgctg
    31141 aacgtaagca tgcaggatcg ccgcaccgtt tgcagactga tcgaccagcg acgcgctgtc
    31201 tgaacccaga cggaacagcg ccaggtacag cagagtcaga ccaacacccg ccatcaggcc
    31261 agcccagacg gtataacggg tcagcagacg cgcttcggta acgccacgag aacgcgccgc
    31321 gttaacaata acgataccaa acaccattgc gcccagcgta tccatggtca gatagccgtt
    31381 gacgaagccg ttagaaaacg cagcgttttg ataagcctca gtcgccgtgc tgatagaacc
    31441 cgccggccag ataattgcgg caacagacag gatgaccagc gcgataattt tcagcggcgc
    31501 aaggaagttg cccacggtat ccagcagctt gcccggatag agcgaaacca gaataacgat
    31561 agcgaaatag accaggctgt aaataaacag cggcagcgcg gaatcacccg tcagcggcgc
    31621 aatgcccact tcaaaagaaa cggtagctgt acgcggcgta gcgaaaagcg gccccaccgc
    31681 caggtaacaa actgttgcca gcagtacgcc agcgacttta ccaattggcg tgctgagact
    31741 gtcaacaccg ccgccaactt ttgccagcgc cactaccgtt aataccggta ggccaacggc
    31801 agtaatgagg aagccgaatg ccgcagtcca gacgtgttcg cctgcctgca agccgaccat
    31861 tggagggaaa ataatgttac ctgcgccgac gaacaacgca aatgtcataa agcccagagc
    31921 gatgatatcg cgcgatctta attgatgggt cataaatcat actgcctgtg gatgtggtgt
    31981 tgtaacgtta aatttttgtg ccaatccctg gggatgatac agcggtatat ttaaacaatt
    32041 ttagcgggaa atactcccaa ccaggcgcgg cgaagaatag tttttcgatt taccgcgtaa
    32101 atgcagtcag tatgacagca cctgaagcgc aatttaaacg cttataacgt tttaaagcaa
    32161 gaagggatct aaaaaccaga ggtatatagc gataagaaat caccaaccag tataaaacac
    32221 tatttcaatg aaaaagacat ggctaatcat gcgaacaaaa aatcaccaac cattgtaaaa
    32281 tctaacgccc gtatttcgac tgcaaagaaa ataagccagc atcggctggc ttatggcaat
    32341 aaaagatgac aaaggcgaat taatcgctgt ttttggcaat taaacgttcc gggataataa
    32401 aactgaaacg tgtacctttt cctactgtac tctcaatatt caggcgactt tcgtgatgat
    32461 tcaccgcatg tttcacgatc gctaacccta atccgctacc accggtttgc cgggaacgcg
    32521 ctttatcaac gcgataaaaa cgctcggtca ggcgcggaat atgctccggt gcaatgcccg
    32581 gtccgttatc ttcaacacta aattcggcac cgtgcggcac tcgctgccag cgtacggtga
    32641 tatgcgtgcc ttccggcgta tgattcacgg cgttatagac caggttcgaa atcgcactac
    32701 gtagctgatc ttcgttgcct gacaccttga ggccgttatc tatctcaaag gtaaatgtct
    32761 gttttttctg actcagagtc tgagcctcgc gctcaacgac gcgcagcatc atcggcacat
    32821 caaccttttc attgagcaaa tgcgtcggtg cggcttctat tttcgacagc gtcagcaatt
    32881 gcttcaccaa cccttccatc cgctgggtct gctcacgcat ggtgtgcaac gctttttcac
    32941 gtaccgcgcc ttccagcggt tgctcatcca tcatctccag gtaaccctgt aacacggtca
    33001 atggcgtacg taactcatgg ctcacgttgg caaaaaagtt acgccgcgcc ccttccagtt
    33061 gatgcatttg cgtgacatct cgcgccacca tcagcaactg tttgtgggta taaggcatga
    33121 cgcgaatctc cagatgccgc ccggtattga gcaccagatt gagcgggcga gagaaatcac
    33181 gcgttttcag atattgcgta aactccgggt aacgcagcaa gttaaggatg ttctgcccgt
    33241 tatcttccgg ccagcgcaag ccaagaatct gttgcgccag accgttgcac cagaaaatac
    33301 cgccctcttc cgtggtcagc accaccgcgt cgggcagcga ctccgcgccg ctacgaaagc
    33361 gtttaatcag attgcccagt tcacggcggc gttttttatt tcgcagctgc atctggtgta
    33421 agccgtatag tagcggttcc cagctaccac gccccggtgg cggggtcata ctacgatcca
    33481 cccacagcca ccatgaaagg cgcaataaat tccagaaatg ccagataagc agtcctgtta
    33541 ccgatgccag caaaaaccag ggcaggtaac ccaaaaatgc acccaggatg aaagccggga
    33601 ggcagcaaaa tagcagctcc agcaccagcc ttttccacga cagccgttcc agcacgtaag
    33661 atactccagt taagaaatca taagccctgc tctacgtccg atgagcaagg ccttaaaagc
    33721 gggttgaaaa acgataccct gtaccgcgca cggtctgcac catgcggtca tgcccgccgg
    33781 gctccagtgc tttacgcagg cgacgaatgt ggacatcgac cgtacgatct tccacataca
    33841 cgttggttcc ccatacgtga tttaacagtt gctcgcgact gtacacgcgc tcgggatgcg
    33901 tcataaaaaa gtgcagcagt ttaaattcag ttggccccat ctccagcggt tcttcgcccg
    33961 ccatcacccg gtgagatgtc ggatcgagac ttagcccctg catctcaatc acctcttcca
    34021 ccgccatcgg cgatatacgg cgcattaccg ctttaattcg cgccaccagc tccttcggag
    34081 aaaacggctt ggtgatatag tcatccgcac cggtttcaag gccgcgcacg cgatcttctt
    34141 cttcccctct ggcggtcaac atcaccactg gaatatcccg ggtcatcgac tcgcgcttga
    34201 ggtgtttgat gaactggata ccggagccgc caggtaacat ccagtcaagg agaattaaat
    34261 ccggccaggg ttcattcagt tgattcacag cactgtcata atcttccgct tcgaccggct
    34321 gaaagccatt ttgttcgagc acgaagcaga ccatttcgcg aattggagct tcatcttcta
    34381 cgaccagaat acgtctcgcc atgatttgcc ctgttgtaat aaataggttg cgatcattaa
    34441 tgcgacgtca ttatgcgtca gatttatgac agatttatga aaagctcgtc gcacatatct
    34501 tcaggttatt gatttccgtg gcacagaaaa aagcaaatgg cacatctgtt tgggtataat
    34561 cgcgcccatg ctttttcgcc agggaaccgt tatgcgcatc cttcacacct cagactggca
    34621 tctcggccag aacttctaca gtaaaagccg cgaagctgaa catcaggctt ttcttgactg
    34681 gctgctggag acagcacaag cccatctggt ggatgcgatt attgttgccg gtgatgtttt
    34741 cgataccggc tcgccgccca gttacgcccg cacgttatac aaccgttttg ttgtcaattt
    34801 acagcaaact ggctgtcatc tggtggtact ggcaggaaac catgactcgg tcgccacgct
    34861 gaatgaatcg cgcgatatca tggcgttcct caatactacc gtggtcgcca gcgccggaca
    34921 tgcgccgcaa atattgcccc gtcgcgacgg gacgccaggc gcagtgctgt gtcccatacc
    34981 ttttttacgc ccgcgtgaca ttattaccag ccaggcgggg cttaacggta ttgaaaaaca
    35041 gcagcatttg ctggcggcta ttactgatta ttatcaacaa cattatgccg atgcctgcaa
    35101 actgcgcggc gatcagcctc tgcccatcat cgccactgga catttaacga ccgtgggtgc
    35161 cagtaaaagt gacgccgtgc gtgacattta tattggcacg ctggacgcgt ttccggcaca
    35221 aaactttcca ccagccgact acatcgcgct cgggcatatt caccgcgcgc agcttattgg
    35281 cggcatggaa catgttcgct attgcggctc ccccattcca ctgagttttg atgaatgcgg
    35341 taagagtaaa tatgtccatc tggtgacatt ttcaaacggc aaattagaga gcgtggaaaa
    35401 cctgaacgta ccggtaacgc aacccatggc ggtgctgaaa ggcgatctgg cgtcgattac
    35461 cgcacagctg gaacagtggc gcgatgtatc acaggagccg cctgtctggc tggatatcga
    35521 aatcactact gatgagtatc tgcatgatat tcagcgcaaa atccaggcat taaccgaatc
    35581 attgcctgtc gaagtattgc tggtacgccg gagtcgtgaa cagcgcgagc gtgtgttagc
    35641 cagccaacag cgtgaaaccc tcagcgaact cagcgtcgaa gaggtgttca atcgccgtct
    35701 ggcactggaa gaactggatg aatcacagca gcaacgtctg cagcatcttt tcgccacgac
    35761 gttgcatagc ctcgccggag aacacgaagc atgaaaattc tcagcctgcg cctgaaaaac
    35821 ctgaactcat taaaaggcga atggaagatt gatttcaccc gcgagccgtt cgccagcaac
    35881 gggctgtttg ctattaccgg cccaaccggt gcggggaaaa ccaccctgct ggacgccatt
    35941 tgtctggcgc tgtatcacga aactccgcgc ctctctaacg tttcacaatc gcaaaatgat
    36001 ctcatgaccc gcgataccgc cgaatgtctg gcggaagtgg agtttgaagt caaagatgaa
    36061 gcgtatcgcg cgttctggag ccagaatcgg gcgcgtaacc agccggacgg taatttgcag
    36121 gtgccacgcg tagagctggc gcgctgtgca gacggcaaaa ttctcgccga caaagtgaaa
    36181 gataagctgg aactgacggc gacgttaacc ggactggatt acgggcgctt cacccgttcg
    36241 atgctgcttt cgcaggggca atttgctgcc ttcctgaatg ccaaacccaa agaacgcgca
    36301 gaattgctcg aggagttaac cggcactgaa atctacgggc aaatctcggc gatggttttt
    36361 gagcagcaca aatcggcccg cacagagctg gagaagctgc aagcgcaggc cagcggcgtc
    36421 gcgttgctca cgccggaaca agtgcaatcg ctgacagcga gtttgcaggt acttactgac
    36481 gaagaaaaac agttaattac cgcgcagcag caagaacaac aatcgctaaa ctggttaacg
    36541 cgtctggacg aattgcagca agaagccagc cgccgtcaac aggccttgca acaggcatta
    36601 gccgaagaag aacaagcgca acctcaactg gcggcgctca gtctggcaca accggcacga
    36661 aatcttcgtc cgcactggga acgcatcgca gaacacagca cggcgctggc gcatactcgc
    36721 cagcagattg aagaagtaaa tactcgctta cagagcacaa tggcgcttcg tgcgagcatt
    36781 cgccaccacg cggcgaagca gtcagcagaa ttacagcagc agcaacaaag cctgaatgcc
    36841 tggttacagg aacacgaccg cttccgtcag tggaacaacg aactggcggg ttggcgtgcg
    36901 cagttctccc aacaaaccag cgatcgcgag catctgcggc aatggcagca acagttaacc
    36961 catgctgagc aaaaacttaa tgcgcttgcg gcgatcacgt tgacgttaac cgccgatgaa
    37021 gttgctagcg cccaggcgca acatgctgag caacgcccac tgcgtcagcg cctggtcgca
    37081 ctgcatgggc agattgttcc ccaacaaaaa cgtctggcgc agttacaggt cgctatccag
    37141 aacgtcactc tggagcagac gcaacgtaat gccgcactta acaaaatgcg ccatcgttat
    37201 aaagaaaaga tgcaacaact tgccgatgtg aaaaccattt gcgagcagga agcgcgcatt
    37261 aaaacgctgg aagcccagcg cgcacagtta caggcgggtc agccttgccc actttgtggt
    37321 tccaccagcc acccggcggt cgaggcgtat caggcgctgg aaccgggcgt taatcaggcc
    37381 cggctattaa cgctggaaaa agaagtgaaa aagctcggcg aagaaggtgc gacgctacgt
    37441 gggcaactgg acgccttaac aaagcaactt cagcgcgatg aaaacgaagc gcaaagcctc
    37501 cgacaagatg agcaagcact tactcaacaa tggcaagccg tcacggccag cctcaatatc
    37561 accctgcagc cacaggacga tattcaaccg tggctggatg cacaagatga gcacgaacgc
    37621 cagctgcggt tactcagcca acggcatgaa ttacaagggc agattgccgc gcataatcag
    37681 caaattatcc agtatcaaca gcaaattgaa caacgccagc agcagctctt aacggcattg
    37741 gcgggttatg cactgacatt gccacaggaa gatgaagaag agagctggtt ggcgacgcgt
    37801 cagcaagaag cgcagagctg gcagcaacgc cagaacgaat taaccgcgct gcaaaaccgt
    37861 atgcagcagc tgacgccgat tctggaaacg ttgccgcaaa gtgatgaact cccgcacagc
    37921 gaagaaactg tggcgctgga aaactggcgg caggttcatg aacaatgtct cgcattacac
    37981 agccagcagc agacgttaca gcaacaggat gttctggcgg cgcaaagtct gcaaaaagcc
    38041 caggcgcagt ttgacaccgc gctacaggcg agcgtctttg acgatcagca ggcgttcctt
    38101 gcggcgctaa tggatgaaca aacactaacg cagctggaac agctcaagca gaatctggaa
    38161 aaccagcgcc gtcaggcgca aacgctggtc actcagacag cagaaacgct gacacagcat
    38221 cagcaacacc gacctggcgg gttgtctctc actgtgacgg tggagcagat tcagcaagag
    38281 ttagcgcaaa ctcaccaaaa gttgcgtgaa aacaccacga gtcaaggcga gattcgccag
    38341 cagctgaagc aggatgcaga taaccgtcag caacaacaaa ccttaatgca gcaaattgct
    38401 caaatgacgc agcaggttga ggactgggga tatctgaatt cgctaatagg ttccaaagag
    38461 ggcgataaat tccgcaaatt tgcccaaggg ctgacgctgg ataatttagt ccatctcgct
    38521 aatcagcaac ttacccggct gcacgggcgc tatctgttac agcgcaaagc cagcgaggcg
    38581 ctggaagttg aggttgttga tacctggcag gctgatgcgg tacgcgatac ccgtaccctt
    38641 tccggcggcg aaagtttcct cgtcagtctg gcgctggcac tggcgctttc agatctggtc
    38701 agccataaaa cgcgtattga ctcgctgttc cttgatgaag gttttggcac gctggatagc
    38761 gaaacgctgg ataccgccct tgatgcgttg gatgccctga atgccagtgg caaaaccatc
    38821 ggggtgatta gccacgttga agcgatgaaa gagcgtattc cggtgcagat caaagtgaag
    38881 aagatcaatg gactgggcta cagcaaactg gaaagtgcgt ttgcagtgaa ataactattc
    38941 agcaggataa tgaatacaga ggggcgaatt atctcttggc ccgactggtc gttatcctgc
    39001 aagctatcac tttattggct acggtgattg gtagccgttc tggtggttgt gatggtggta
    39061 tgaaaaaagt tattttatct ttggctctgg gcacgtttgg tttggggatg gccgaatttg
    39121 gcattatggg cgtgctcacg gagctggcgc ataacgtagg aatttcgatt cctgccgccg
    39181 ggcatatgat ctcgtattat gcgctggggg tggtggtcgg tgcgccaatc atcgcgcttt
    39241 tttccagtcg ctactcactc aaacatatct tgttgtttct ggtggcgttg tgcgtcattg
    39301 gcaacgccat gttcacgctc tcttcgtctt acctgatgct cgccattggt cggctggttt
    39361 ccggcttccc gcatggtgca ttttttggcg tcggcgcgat cgtgttatca aaaattatca
    39421 aacccggcaa agtcaccgcc gccgtggcag ggatggtttc cgggatgaca gtcgccaatt
    39481 tgctgggcat tccactggga acgtatttaa gtcaggaatt tagctggcgt tacacctttt
    39541 tgttgatcgc tatttttaat attgcggtga tggcatcggt ctatttttgg gtgccggata
    39601 ttcgcgacga ggcgaaagga aaactacgcg aacaatttca ctttttacgc agccctgccc
    39661 cgtggttaat tttcgctgct acgatgtttg gcaacgcagg tgtgtttgcc tggttcagct
    39721 acgtaaagcc atacatgatg tttatttccg gtttttcgga aacggcgatg acctttatta
    39781 tgatgttagt tgggctaggg atggtgctgg ggaatatgct aagtggcagg atttcaggac
    39841 gttattcacc actgcgcatt gcagcagtga ctgactttat cattgtactg gcactgctga
    39901 tgctcttttt cttcggcggt atgaaaacaa cgtcgcttat ttttgctttt atttgttgcg
    39961 cgggattatt tgccctttca gcacctctac aaatattatt actgcaaaat gccaaaggcg
    40021 gagagttatt aggtgccgca ggtgggcaaa tagcgtttaa cctcggtagc gccgtcggcg
    40081 catattgcgg tggtatgatg ctgacgctgg ggctggcata taattacgtg gcgctgcccg
    40141 ccgccctgct ttcgtttgct gcgatgtcgt cgttgctgct gtatggtcgc tataagcgcc
    40201 agcaagcggc ggatagtccg gtgctggcga aaccactggg gtagattgca gtcacggtgc
    40261 acgttgctta ttgccggatg cggcgtgaac gccttatccg gcctacggga ctgttcattt
    40321 tgtaggcctg ataagacacg ccagtgtcgc atcaggcatc ggagcaccgg ggggttgccg
    40381 ccactgtgcg cgttatttgt tgccggatgc ggcgcgagcg ccttatccgg cctaccggac
    40441 ggctcacttt gtaggcctga taagacgcgg caagcgtcgc atcaggcatg atacgccaat
    40501 tgcctgggtt ttttactctt gtggccataa ccatgcagcg ccgcgtacgc cgctcgaatc
    40561 accatgtttc gccttgtgta ccggcgtttc acattctccg ccgaagacaa attgtttaat
    40621 caactgcgga accgtttgat ataaacggtc tacattgctc attccgccgc ccaggacaat
    40681 cacatccgga tcgagaatat tcacgacatg tgccagcgat tttgccagcc gcagctcgta
    40741 gcgacgcaat gccagttccg ctaccggatc gctttcttca accaggcgga taatttcact
    40801 gcctttcagc gcatgtccgc tcaaacgacg ataatccgtc gcgaatcccg tgcctgaaat
    40861 aaaggtttca atacaacctt gtttaccgca ataacaaggg acttcctcgc gataacgcag
    40921 ttcgtcttcg tccatccacg gtagcggatt gtgtccccac tcgcctgccg tgccattgcc
    40981 gccgatatgc gcccgcccat tgaatgccac gcccgcgccg catcccgtgc cgataatcac
    41041 ggcaaaaacc gtctgcgctc ccgctgccgc gccatctacc gcttctgaaa ccgccagaca
    41101 gttggcgtca tttgccagcc gcacttcccg ctgcaaccgc gcgcttaagt ctttatcgaa
    41161 tggctgaccg ttgagccagg ttgaattggc attcttcacc acgccggtgt agggcgaaat
    41221 cgaacctgga atgcccatac ctaccgttcc gcgctgccct gttgcctgct ccgccatatc
    41281 aaccaacgtg gcgatcgttt caatagtctg ccggtaatca tcacgcggcg tgggcagacg
    41341 atggcggtac aactgctccc ctgcatcgcc caatgcaatc acttcagttt tggtgccgcc
    41401 taaatcgata cctatacgca cggtactctc cttatttttt tcaatatcaa tagcgtagag
    41461 acggacaacc ggattggcaa tgcaaggccg ccgacaattc gttatcatgc ccgctaaatt
    41521 taacgacaag gccgtggaaa ttatcatgct gtggttcaaa aatttaatgg tttaccgtct
    41581 tagccgcgag atttcgctgc gtgcagaaga gatggaaaaa cagctagcct cgatggcatt
    41641 taccccatgc ggcagccagg acatggcgaa gatgggctgg gtacctccga tgggatcgca
    41701 cagcgatgcg ttaacgcacg ttgccaatgg tcaaattgtt atctgtgcgc gcaaagaaga
    41761 aaaaatcctc ccgtccccgg tgattaaaca ggcgctggaa gcgaaaatcg ccaaactgga
    41821 agcggaacag gtgcgtaagc tgaagaaaac cgaaaaagat tcgctgaaag acgaagtgct
    41881 gcactctctg ctgccgcgtg ctttcagccg ttttagccag acaatgatgt ggatcgatac
    41941 ggttaacggt ttgattatgg tggactgcgc cagcgccaaa aaagcggaag atacgctggc
    42001 actgctgcgt aaaagcctgg ggtcgttacc ggttgtacca ttgagcatgg aaaacccgat
    42061 tgaactgacg ctgaccgaat gggtacgttc cggcagcgcg gcacagggct tccagctgct
    42121 tgatgaagcc gagctgaaat cgttgctgga agatggcggc gtgatccgcg cgaagaaaca
    42181 agatctgacc agcgaagaga tcaccaacca cattgaagcc ggaaaagtgg tgactaaact
    42241 ggcgctcgac tggcagcagc gcattcagtt tgtgatgtgc gacgatggtt cgctcaagcg
    42301 tctgaagttc tgcgacgagc tgcgcgatca aaacgaagat atcgaccgtg aagatttcgc
    42361 ccagcgcttt gatgccgatt tcatcctgat gactggtgaa ctggcagcgt taattcaaaa
    42421 cctgattgaa ggattaggtg gcgaagcaca acgttaattg ctgatttacc tttaatgccg
    42481 gatgcgacgc ctgccgcgtc ttatccggcg tacgaagcca caccaggaat ataatcgtta
    42541 gctacagcgg gcaataattc ttagcgcagc aatattatgc gttttatgct gtaacttgct
    42601 ccaggggcgt tgtgtcattg tttttcctca agccggtgac gcgcttcact gcgaattttt
    42661 cgcgttgccc cggttttcac taaatcacgt aacagcgaaa gatcgggttg ttcgacaaat
    42721 aagagtaatg atggatcctc ttttaaccac gccgttttca catcggcagc cagctgtgaa
    42781 ttattaatag ccagcgaccg ctcctgaggc tcagtcaatg tcgttaacaa caacaccggg
    42841 gtatgtgcgt tgtgcaatgc cgtttggcgc aaatgatcat tgcgactttg cagcaatgca
    42901 cgccaccatt catcatctga tttatctttt atcgtcgcca gttccgcaac aatatctgga
    42961 gatcttcgct gatattgata tcaaccttga tgaattatga atatatcgga aatcgaaaaa
    43021 atcccagcaa aggaaatatt attatgggtt aaagagagga catttcagaa aaatgccctc
    43081 ccgttcaagg ggaaggcgag gaattacaga tagcggcaca gataagaggt gggttcggca
    43141 acttgcagat gaaactcgct gtgaccggga acattaaaca ccgaaccggc ttcatacact
    43201 tgccagtcgg tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct
    43261 tcaggctcag cggtgctgaa ggtgtattca ccttcaacca taacacccac gctggcgcga
    43321 ccagtgctgc tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt
    43381 tgaagcataa actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg
    43441 cgctggctgt cacgcaaaat tacatcagta attccgcagc cagccgtgca atcaatacgt
    43501 tagagagcaa gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgctggttaa
    43561 atcccaaaca atccagcatg atgacatctg caccttttgc cagtaattct ttcccggcat
    43621 caatgatttt ttgttccgaa tcatgaatgg ggttacccaa tgaaaatact ggcggtttct
    43681 gcaaaatttg ccatttttgc gcctgaacgg tcagcaactc ctcaaccggt acgataaccc
    43741 ccacctgatg atcttcaaca atagaggaaa ccagcggtgg caatatccgc gacggctcaa
    43801 gaaagatcgt attacgcgcg gtcatactac taatgtttgc tgtactcatt aatataatga
    43861 cgtcgtaacc ctgattatcg agcacttcaa ccacaccttg caggtctcgc tcaactttgc
    43921 gacgcgaaac atgggccaga tggttgtcat ttaataatgt gagaattgtg tcttcgcctg
    43981 cttctggcgc gtactccgcc atcacttctt cgcggcttaa cttccccagc aggctatgat
    44041 gggaaatatt atcttcgtca atgtattctg tcaggagcgg caaaacttcc tgcataggta
    44101 caatgccgat agtcaggatc gccaacgacg cactcattgt tcttccacct tacttttatt
    44161 tcgtcgttaa ttaccgggca agtgttaagc accactgctg acacttactt gtgctacaaa
    44221 tgacaaaaag cgtagcagca gacctgcact gcataatgta aagattccaa atgattccag
    44281 taatggattg ttattgttta atattctaat tatttgaaaa acatgaattg tgaaaaatgt
    44341 gacgcagatc ccttgtggcg gcactcagaa tcccttccga gcggtctgat gcggtcactc
    44401 tttgtcaggg tcctcatagc gttttttcgc atccatcgct tcctgagcgg tcggatgttc
    44461 actgatcaac gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg
    44521 tcctggcttt cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt
    44581 tggcatcgtt cttccttatt tcacgggatg aacgttaagt ataggcgctc gaaaatcaac
    44641 aattgatcgt ctgtgccaga gcgctgcgaa tttcagaaat cacctggctg ggttcgtttg
    44701 ttgcgtcgat gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt
    44761 cctgaacttc ttcgctcagt ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg
    44821 cagcttgcag tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt
    44881 tattttgcat gaagtgacga ttaaattccg tcagaataat gccgccgccc gtggcgataa
    44941 cggtggatgg cgcagttacc gcttccagtg ccgccgtttc tctggcgcgg aatcccgccc
    45001 actcttccct ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactggt
    45061 cggtatcgac aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac
    45121 cacagccccg aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg
    45181 tcgcaatgcg gcgaaagccg gtgtcatgag aatggcgatc ataccatcaa actagtacaa
    45241 tttcgattgt aaagaaaaaa ttccacttaa agtgaaaatc tcaatacatc ccttactata
    45301 ccaataaata ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg
    45361 cacttccagc caccacttct ccagctccgc agcaaaagcc tggcggtcac gttgtgaaag
    45421 actatctggt cctccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat
    45481 cgtcaggcgc tcacgaatgg tcgctggcgt ataacgttcg ccgcgcggat taagcgccgc
    45541 cgcgcctttc tcaatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc
    45601 cgcttcacac tgccgaacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag
    45661 cgtacgaata aatcgtgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg
    45721 catctgcata cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc
    45781 ggcatccacc caaattgtca taaagtcatc ctttgttggg taatcctcta ttgtgtcgcg
    45841 cttttgcctt ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat
    45901 ttcacggcag gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg
    45961 gaaaagccat tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg
    46021 tatatacccc ctccccggat aaagtcaccg ccctgcatga acagttcggc atcaacgccg
    46081 cagaatcggc gcaagaagta gcgcaaatcg ccgacatcat ttttgccgcc gttaaaccag
    46141 gcatcatgat taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg
    46201 tttctattgc tgcaggtatc acgctcgacc agcttgcccg cgcgctgggc catgaccgga
    46261 aaattatccg cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa
    46321 cgccaaacgc gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct
    46381 ttggcgaagc ggaagtgatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt
    46441 cttcgccagc ctacgtcttt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg
    46501 ggatgccacg cgcccaggcg tataaatttg ccgctcaggc ggtaatgggc tccgcaaaaa
    46561 tggtgctgga aacgggagaa catccgggaa cactgaaaga tatggtctgc tcaccgggag
    46621 gcaccaccat tgaggcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg
    46681 aagcgatgac gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tggcttttgc
    46741 cggacgtcag gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtagagcgc
    46801 caaatctgcc gatttcaacc actcgcgata gtgactcatt tgtggattca gcggcgcaac
    46861 ccccacacta atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc
    46921 ttcatgcacc cgtaacatgg cggtgatggc gctctcagct ggcgtaccgg acatgattac
    46981 tgcaaactca tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa
    47041 ctgtcgggta agcgccacaa tcgcttcatc acccacatca tggccccagg tatcgttgat
    47101 gctcttgaaa tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca
    47161 gttatcaaat tcattgcgta acatagtttc ccaatgacgt ctgttataca cgcccgtcat
    47221 accgtcgcgg gtactcatga cctgcaacct gcgtttatgt tccgccagtt tagtcgccgt
    47281 ctggtagctg acccagccaa acagcagagg ataaatgaca ataatgggaa gggagagcca
    47341 ccattccagc ggtgcgctat tgaacgacac ggtgatgccc gtcagttcga gggtgacaag
    47401 gcaggaaacc accatcaaca ccagacccgc aacaaacaga cgggggccgc ctgcccccat
    47461 caaattcaga cacataatca tcaacatcgc ggtggaaggc agcacgttta cgcccattac
    47521 gcctacccac attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag
    47581 cggatcgacg gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc
    47641 gaccaacacc agccaccacc agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg
    47701 taagaacatg ccagccagac caaccgcgcg gggtagtctg acgcgacggg cgaagcgcag
    47761 cccggaccgc tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc
    47821 ttttttgaaa aagttttcat cattcattat ttttgggaac attcagaaac aattttccca
    47881 aattatagag acgggactaa aggcagaagt atgaaatttc gggtgagcca ttttcgtagc
    47941 tcacccgtta gcactatcag gtatcaggcg gctttcttga ggcaggcact cataaactta
    48001 ttacgatcat cccctttcag agattgttgt gttgcttgat tattacattc gcgcatcttt
    48061 tgctgctgtg gcgtcaaact tttttcacca ggcgcagatt tgctgttctt caggcaatca
    48121 ctcatgtagg tcttacgagc atcccctttc aacgcctgcg ccgtcgcctg ctgattacaa
    48181 gaggtcatac gctgttgttg tggggttaag gttctctcgg cagcgccgac ggtggttaaa
    48241 aaaaccagac cgaaaagcaa ggtaaccagt aatgtaattt tcatcgcacc atccctcttc
    48301 atgttttaac catgagcgta tgcgcccgtg atctgtcatt aagtctggtt gctaacagca
    48361 aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg ttttatttca gccccagggc
    48421 ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt ccaacaacat tggcggcatg
    48481 cgggccatac gccgcaatac gcaactgact gccggtatgt tcttgtgaat cctcttcgga
    48541 gttcccgtaa ctcatcacca tcactgcgcc atctttggta tttagcgcct gggtgaggcc
    48601 cggagctttg gtatctggcg caactatctg gctggcatgg gcgtgatcag cggtgactat
    48661 gaccaacgtg ttaccatcct ttttagcgaa ttccagcgcc cgttgtacgg cttcatcgag
    48721 atcgaccgtc tcgccaattt gcccacaagg attcgcagcg tgatcctgtt tatcgattga
    48781 cgcaccttca acttgcagga aaaagccttt ctcattttta ctcaacaatt caatggcttt
    48841 gtcggtcatc tgcgccaggg tcggtacact gtcattacgt tgcggattag gcgtacaggt
    48901 gactgcgggc ttatctatat taccgtggta cgttgctttc ggtccttgcc agcgcactgg
    48961 catattgccg tcagcaaaca gtcctaatag gggtttttgc tgattcgctt ccgtcaccga
    49021 attcagtgag gcagcatcgc tcaccaactg ataaccacgc gcctgtgcct gttcacgcag
    49081 cgtttttccc tgccattcac cggcggttgc cgtttcagca aaggtttttg caccgccgcc
    49141 aagcgtaaca tcggcacggg cgttaagcag ctgttcggta atcgatcctt ttccgccttt
    49201 ttccagagcg ttacccggac atttttcact ggtcgcactt ggaccgtagc atttgcgcga
    49261 ggtcacatgc gccaccagcg cagcgggcgt ggcatcctgc aactctgcgg tagaaacgtt
    49321 accggtcgcc agacctgcgg cttttgccat ttccagaatc gttgggtgat ctttttcgtg
    49381 aatatcgacg cccagcgcgc cgttataggt tttgacacca gttgaccagg cggttgctga
    49441 tgcagccgag tcggtgacgt agtccggttt gccggttttt ttattcagcg catagtgagt
    49501 gtattgcccg gtaagcggta aggcatcgat acctttaaaa aagccgcccg caccttcggc
    49561 ataattgcgt gcggcagtaa tttccgagtc ccccatccca tcgccaatca gcaaaataat
    49621 attttttgca ggtttatcgc taagagaatc acgcagagca gcggtctgat cacccgttaa
    49681 gcggcgagca ccgccgggtg cagtaatatc gccctgagct gcccggtttt ccagaacagg
    49741 catttctggt gtccgggctt ttgtcacagg ggtaaacagt aacggtaaga gtgccagtgc
    49801 aatagtgctt tgtttcactt tattttctcc atgtacaaat acattaaaaa ataaaaacaa
    49861 agcgactata agtctcggcc gtgacaactt tatgacagct gttgaaaaga ttaacttttt
    49921 actgacgagg atgcctcaat aacttcttta cgtaatcgcg cagcagctcc gtatcgtcgt
    49981 caggaatgct ggcatcgggc tttacctcgt acagcgcccc ctctacctga tcaatcaacc
    50041 gctgttgatc attttgcgcc atattgcgaa gcattgccgt gacgataatc tccaaagctt
    50101 ctacctgcgc acacagttct ttcgactctt cttctttttg ggcaagctta aataacaact
    50161 cagcaatgag atttttcatg tctgtatttc cttatccaaa gtatggagaa gttaacatta
    50221 tgtttcacaa cttcatagtg tctggaagta ttatttttat gaca
//
LOCUS       DACADF010000012        49772 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.012, whole genome shotgun sequence.
ACCESSION   DACADF010000012 DACADF010000000
VERSION     DACADF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.012"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            37..141
                     /locus_tag="ISB30_000706"
     CDS             37..141
                     /locus_tag="ISB30_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAP1988383.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            451..2649
                     /gene="speF"
                     /locus_tag="ISB30_000707"
     CDS             451..2649
                     /gene="speF"
                     /locus_tag="ISB30_000707"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HAP1988384.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     HGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSALLKGEKL"
     gene            2646..3965
                     /gene="potE"
                     /locus_tag="ISB30_000708"
     CDS             2646..3965
                     /gene="potE"
                     /locus_tag="ISB30_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAP1988385.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4022..5662)
                     /gene="pgm"
                     /locus_tag="ISB30_000709"
     CDS             complement(4022..5662)
                     /gene="pgm"
                     /locus_tag="ISB30_000709"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAP1988386.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5688..6233)
                     /gene="seqA"
                     /locus_tag="ISB30_000710"
     CDS             complement(5688..6233)
                     /gene="seqA"
                     /locus_tag="ISB30_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAP1988387.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFTAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6418..7182
                     /gene="ybfF"
                     /locus_tag="ISB30_000711"
     CDS             6418..7182
                     /gene="ybfF"
                     /locus_tag="ISB30_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP1988388.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7253..7615
                     /gene="ybfE"
                     /locus_tag="ISB30_000712"
     CDS             7253..7615
                     /gene="ybfE"
                     /locus_tag="ISB30_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAP1988389.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            7755..8285
                     /gene="fldA"
                     /locus_tag="ISB30_000713"
     CDS             7755..8285
                     /gene="fldA"
                     /locus_tag="ISB30_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAP1988390.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8495..8581
                     /gene="uof"
                     /locus_tag="ISB30_000714"
     CDS             8495..8581
                     /gene="uof"
                     /locus_tag="ISB30_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAP1988391.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8574..9020
                     /gene="fur"
                     /locus_tag="ISB30_000715"
     CDS             8574..9020
                     /gene="fur"
                     /locus_tag="ISB30_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAP1988392.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9104..9430)
                     /locus_tag="ISB30_000716"
     CDS             complement(9104..9430)
                     /locus_tag="ISB30_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988393.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9480..10886)
                     /gene="chiP"
                     /locus_tag="ISB30_000717"
     CDS             complement(9480..10886)
                     /gene="chiP"
                     /locus_tag="ISB30_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAP1988394.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSANDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11328..12992)
                     /gene="glnS"
                     /locus_tag="ISB30_000718"
     CDS             complement(11328..12992)
                     /gene="glnS"
                     /locus_tag="ISB30_000718"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAP1988395.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13212..13973)
                     /locus_tag="ISB30_000719"
     CDS             complement(13212..13973)
                     /locus_tag="ISB30_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1988396.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(13975..14961)
                     /locus_tag="ISB30_000720"
     CDS             complement(13975..14961)
                     /locus_tag="ISB30_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HAP1988397.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAVAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTGSKEYAMVLYTEKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(14954..16189)
                     /locus_tag="ISB30_000721"
     CDS             complement(14954..16189)
                     /locus_tag="ISB30_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HAP1988398.1"
                     /translation="MEKGITKPSILIVADDFTGANDAGVSLAQVGHTVDVAFEMPYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTAIPCQPVTVSQLSHLSYEQPWIGVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPRITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     CAMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKRSE"
     gene            16359..17516
                     /locus_tag="ISB30_000722"
     CDS             16359..17516
                     /locus_tag="ISB30_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP1988399.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDALGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFAQVWDCIPDAETALSAEEKSHALVTWLQALVNQLKLPNNL
                     ADLGVPPEDIASLSEAALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
     gene            17520..18407
                     /locus_tag="ISB30_000723"
     CDS             17520..18407
                     /locus_tag="ISB30_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HAP1988400.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCVDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADTLSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSDGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSACISGLANVAPREI
                     NAIWSRFHAGDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQISEIQQIIRHYNIN"
     gene            18467..19225
                     /locus_tag="ISB30_000724"
     CDS             18467..19225
                     /locus_tag="ISB30_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAP1988401.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERKNKKVSTLIETHESHSH"
     gene            19261..20430
                     /locus_tag="ISB30_000725"
     CDS             19261..20430
                     /locus_tag="ISB30_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP1988402.1"
                     /translation="MSVALIRDGVIRPSENDSHVLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAAKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHDGTLVALF
                     RSRWADNIYISHSVDNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     ERRASLYDEIDDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWSWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            20427..20897
                     /locus_tag="ISB30_000726"
     CDS             20427..20897
                     /locus_tag="ISB30_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAP1988403.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCSLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            20911..22191
                     /locus_tag="ISB30_000727"
     CDS             20911..22191
                     /locus_tag="ISB30_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane transport protein"
                     /protein_id="HAP1988404.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATVLETAGFHQAISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIGVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(22280..24226)
                     /gene="nagE"
                     /locus_tag="ISB30_000728"
     CDS             complement(22280..24226)
                     /gene="nagE"
                     /locus_tag="ISB30_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAP1988405.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQIPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            24559..25359
                     /gene="nagB"
                     /locus_tag="ISB30_000729"
     CDS             24559..25359
                     /gene="nagB"
                     /locus_tag="ISB30_000729"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP1988406.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25419..26567
                     /gene="nagA"
                     /locus_tag="ISB30_000730"
     CDS             25419..26567
                     /gene="nagA"
                     /locus_tag="ISB30_000730"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP1988407.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            26576..27796
                     /gene="nagC"
                     /locus_tag="ISB30_000731"
                     /gene_synonym="nagR"
     CDS             26576..27796
                     /gene="nagC"
                     /locus_tag="ISB30_000731"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAP1988408.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27844..28596
                     /gene="nagD"
                     /locus_tag="ISB30_000732"
     CDS             27844..28596
                     /gene="nagD"
                     /locus_tag="ISB30_000732"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HAP1988409.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            28852..30516
                     /gene="asnB"
                     /locus_tag="ISB30_000733"
     CDS             28852..30516
                     /gene="asnB"
                     /locus_tag="ISB30_000733"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAP1988410.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(30558..30995)
                     /locus_tag="ISB30_000734"
     CDS             complement(30558..30995)
                     /locus_tag="ISB30_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988411.1"
                     /translation="MLRFMTALMGALLLMQSAFADTVKPEIGKYVFGYRGQEGAVVWM
                     MRIGPKASNEALIQVSHVDNDIDGQIFRCKVKALQEGEKSYTAVIKGKSFELLRLKEG
                     NGRLHIPDEQATWLVAYSDELSNSDVANPEHFLTAYQNQQAPQ"
     gene            31329..31405
                     /locus_tag="ISB30_000735"
     tRNA            31329..31405
                     /locus_tag="ISB30_000735"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31363..31365,aa:Met,seq:cat)
     gene            31416..31500
                     /locus_tag="ISB30_000736"
     tRNA            31416..31500
                     /locus_tag="ISB30_000736"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31450..31452,aa:Leu,seq:tag)
     gene            31524..31598
                     /locus_tag="ISB30_000737"
     tRNA            31524..31598
                     /locus_tag="ISB30_000737"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31556..31558,aa:Gln,seq:ttg)
     gene            31633..31707
                     /locus_tag="ISB30_000738"
     tRNA            31633..31707
                     /locus_tag="ISB30_000738"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31665..31667,aa:Gln,seq:ttg)
     gene            31723..31799
                     /locus_tag="ISB30_000739"
     tRNA            31723..31799
                     /locus_tag="ISB30_000739"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31757..31759,aa:Met,seq:cat)
     gene            31848..31922
                     /locus_tag="ISB30_000740"
     tRNA            31848..31922
                     /locus_tag="ISB30_000740"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31880..31882,aa:Gln,seq:ctg)
     gene            31960..32034
                     /locus_tag="ISB30_000741"
     tRNA            31960..32034
                     /locus_tag="ISB30_000741"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31992..31994,aa:Gln,seq:ctg)
     gene            complement(32189..33364)
                     /gene="ubiF"
                     /locus_tag="ISB30_000742"
     CDS             complement(32189..33364)
                     /gene="ubiF"
                     /locus_tag="ISB30_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAP1988412.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLTALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            33510..34934
                     /gene="miaB"
                     /locus_tag="ISB30_000743"
     CDS             33510..34934
                     /gene="miaB"
                     /locus_tag="ISB30_000743"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAP1988413.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTDDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            35048..36127
                     /locus_tag="ISB30_000744"
     CDS             35048..36127
                     /locus_tag="ISB30_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP1988414.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            36124..36591
                     /gene="ybeY"
                     /locus_tag="ISB30_000745"
     CDS             36124..36591
                     /gene="ybeY"
                     /locus_tag="ISB30_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP1988415.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            36681..37559
                     /gene="corC"
                     /locus_tag="ISB30_000746"
     CDS             36681..37559
                     /gene="corC"
                     /locus_tag="ISB30_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAP1988416.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37584..39122
                     /gene="lnt"
                     /locus_tag="ISB30_000747"
     CDS             37584..39122
                     /gene="lnt"
                     /locus_tag="ISB30_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAP1988417.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSTAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYVQWFTPQP
                     EKTIQVSMVQGNIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLITGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYSRTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(39249..41126)
                     /locus_tag="ISB30_000748"
     CDS             complement(39249..41126)
                     /locus_tag="ISB30_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP1988418.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSDESWRLLSSVFLHSSFSHLLMNMFALLVVGTVAERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGTRQTGIDNACHIGGLIAGGALGWLSARLVGQNRLVTEGGIIV
                     AVTLLLTGTIWLVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDANTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDETLLYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGMSGKPGAWV
                     MALSPDEKTLLVPGAGRGNIVRINTITHQKEDFPAGDARGVVSAMGFRPKNGEIIFAD
                     SQGISRIRAEDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRESSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            41580..42488
                     /gene="gltI"
                     /locus_tag="ISB30_000749"
     CDS             41580..42488
                     /gene="gltI"
                     /locus_tag="ISB30_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAP1988419.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGNTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSNTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            42658..43398
                     /gene="gltJ"
                     /locus_tag="ISB30_000750"
     CDS             42658..43398
                     /gene="gltJ"
                     /locus_tag="ISB30_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAP1988420.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            43398..44072
                     /gene="gltK"
                     /locus_tag="ISB30_000751"
     CDS             43398..44072
                     /gene="gltK"
                     /locus_tag="ISB30_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAP1988421.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            44072..44797
                     /gene="gltL"
                     /locus_tag="ISB30_000752"
     CDS             44072..44797
                     /gene="gltL"
                     /locus_tag="ISB30_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAP1988422.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIIEDS
                     PKDAFFDNPKSDRAKDFLAKILH"
     gene            44915..45850
                     /gene="rihA"
                     /locus_tag="ISB30_000753"
     CDS             44915..45850
                     /gene="rihA"
                     /locus_tag="ISB30_000753"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAP1988423.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            45934..47604
                     /locus_tag="ISB30_000754"
     CDS             45934..47604
                     /locus_tag="ISB30_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAP1988424.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVNASAGDNFLGGEDFTHMLVDEVLKRA
                     EVAKTTLNDSELAALYACVEAAKCSNQSPMHIRWQYQDEMRKCEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRNEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGTYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(47664..49115)
                     /gene="djlC"
                     /locus_tag="ISB30_000755"
     CDS             complement(47664..49115)
                     /gene="djlC"
                     /locus_tag="ISB30_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAP1988425.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAATEHEILRVFRALLDSENDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDCEGIDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFSLERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAQQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLDARLERGEAHPLETIITRMLLTKVKLEPEDEDVDEPTPENHEEIND
                     EDEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(49112..>49772)
                     /locus_tag="ISB30_000756"
     CDS             complement(49112..>49772)
                     /locus_tag="ISB30_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAP1988426.1"
                     /translation="EILNQGCKPAHDSPKMYTGIKEFDLSSSWGINNRDDLIQTIYQM
                     TDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQTAMCCGEGGIKAWD
                     YVRMGFLSRVGVLNKWLTEDESLWLQSRVYIRAHHYYHSWMHYFSAYSLGRLYWQSSQ
                     CEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQPECPVTLKDVSD
                     L"
BASE COUNT        12118 a        12863 c        12492 g        12299 t
ORIGIN      
        1 cctaccttca actgacacag aaaaaggtaa aggtaaatgg aaaataataa ccgcttaatg
       61 cctcatataa ggcggacaac ccatatcatg aagtttgccc atcgtaatag ttttgacttt
      121 catttcttta atgcccgcta gtcttctgac taaagggcac cccagcgtac aggtctccct
      181 gactttaagc attacaggtt aatacctgta tccctcggtg ctcatatact gctaaccctt
      241 ttaaactcta aataattcga gtcgcagcac atgcaacttg aagtatgacg agtatagcca
      301 gttaccgggc tggtctgggt tattgcatct gcaaaaagca aactactgat ttatttatca
      361 gcggtggggt tttgcttttt tccggcgtga tcaatttctc ctttgagaaa ttgaggacct
      421 gctattacct gaaataaaga gatgaacaaa atgtcagaat taaaaattgc cgttagccgt
      481 tcttgcccgg attgtttttc cactcatcgt gaatgcgtga atattgacaa aagtaattat
      541 attgacgttg ccgccattat tttatcagtc aatgatgttg aacatggaaa actcgatgaa
      601 atagacgcta ctggctatgg cattcctgtt tttattgcaa cggaaaatga agaacgtgta
      661 ccagcagaat atctgccacg tatttctggt gtcttcgagc attgcgaatc acggaaagag
      721 ttttatggac gtcagttaga aaccgctgcc agccattatg aaacgcaact gcgcccgccc
      781 ttcttccgcg cactggtcga ttatgtcaat cagggcaata gcgcttttga ctgcccgggt
      841 catcagggag gtgagttttt ccgtcgccat ccggcaggca atcagttcgt ggaatatttt
      901 ggcgaaatgc tcttccgctc cgacttgtgt aatgccgacg ttgcaatggg cgatctactg
      961 attcatgaag gtgcgccatg cattgcacag caacatgcgg ctaaagtgtt taatgccgat
     1021 aaaacgtact ttgttttaaa cggcacatcc tcctccaaca aagtggtttt aaacgccctg
     1081 ctgacaccgg gtgatctggt gctgtttgac cgtaataacc ataagtctaa ccaccacgga
     1141 gcgttgctac aggctggtgc aacaccggtt tatctggaaa cagcacgtaa cccgtatggt
     1201 tttatcggtg gcattgatgc gcactgcttc gaagaaagtt atctgcgtga actgattaca
     1261 gaagttgcac cgcaacgggc aaaagaggcg cgtccgttcc gcctcgctgt gattcagtta
     1321 ggcacctacg acggtactat ttataacgcc cgccaggtgg tggataaaat cggccatctg
     1381 tgtgactaca tcctgtttga ctccgcatgg gtcggctatg aacagtttat tccgatgatg
     1441 gcggactgtt cgccgctgct actggagctt aatgagaacg atccgggtat tctggttacg
     1501 caatctgtgc ataaacaaca ggctggtttt tctcagactt cacaaattca taaaaaagac
     1561 agccatatca aagggcaaca gcgttatgta ccgcacaaac gcatgaacaa cgcctttatg
     1621 atgcacgcct ccaccagccc gttctatccg ctgtttgccg cactggatat caacgccaaa
     1681 atgcatgaag gtgtcagcgg tcgtaatatg tggatggatt gtgtggttaa cggtattaat
     1741 gcccgcaaat tgatcctcga taactgccag cacattcgcc cgttcgttcc tgaactggtg
     1801 gatggtaaac cctggcagtc gtatgaaaca gcgcaaattg cggttgatct gcgcttcttt
     1861 aaatttgtac ccggtgagca ctggcattct tttgaaggct acgcagagaa ccaatacttt
     1921 gtcgatccgt gcaaactgtt gctgacaact ccaggtattg atgcacgtaa cggcgaatat
     1981 gaagcgttcg gtgtacccgc aacgattctt gctaacttcc tgcgcgaaaa tggcgtagtg
     2041 ccggaaaaat gcgatcttaa ctccatcctc ttcctgctga ctccggcaga agatatggcc
     2101 aaactgcagc aacttgttgc cctgctggta cgcttcgaaa aactactgga agctgatgcg
     2161 cctctggcgg aagtactgcc ttccatctac aaacagcatg aagagcgcta cgccggttat
     2221 accctgcgtc agttgtgcca ggaaatgcat gatttgtatg cccgtcacaa cgtgaaacaa
     2281 ctgcaaaaag agatgttccg taaggaacac ttccctcgcg tcagcatgaa tccgcaagaa
     2341 gccaactacg cctacttacg cggtgaagtg gagctggttc gtctgccgga tgcagaaggc
     2401 cgtatcgctg ccgaaggtgc gcttccttat cctccgggtg tgctgtgtgt tgttcctggt
     2461 gaaatctggg gtggtgctgt tctgcgttac ttcagcgctc tggaagaagg catcaacctg
     2521 ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg aagaacatga tggtcgtaag
     2581 caagtttggt gctatgtcat caagcctcgt gatgcgcaaa gcgccctgtt gaaaggggaa
     2641 aaattatgag tcaggcgaaa tcgaacaaaa tgggtgtcgt tcagttaacc atactgacga
     2701 tggtcaacat gatgggctcc ggtatcatca tgctgccgac aaagcttgcc gaagtcggga
     2761 caatctcaat tatctcctgg ctggtgacag ccgtaggctc aatggcactg gcatgggcat
     2821 tcgcaaaatg cggtatgttc agccgtaaat caggaggtat gggcggttat gccgagtatg
     2881 ctttcggtaa atccggtaac tttatggcga actataccta cggcgtctcg ctgctgattg
     2941 ctaacgtcgc gattgctatt tcggcggttg gttacggcac cgaactgctc ggcgcgagtt
     3001 tgtcgccagt gcagattggt cttgcgacca tcggggtgct gtggatttgt accgtggcta
     3061 actttggtgg tgcgcgcatt accgggcaaa tcagtagcat taccgtgtgg ggggtcatta
     3121 ttccggtggt aggcctgtgc attatcggct ggttctggtt tagcccgacg ctttacgttg
     3181 attcctggaa tccgcatcat gcacccttct tcagtgcggt aggttcttcc atcgctatga
     3241 cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa tactgatgta gtggaaaacc
     3301 cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt aggtgcggcg gtgatttata
     3361 tcgtctccac caacgtgatt gccgggattg tgccaaatat ggagctggca aattcaacgg
     3421 caccatttgg tctggccttc gcgcagatgt tcacgccgga agtgggtaaa gtcattatgg
     3481 cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg gcagttcacc attgcccagg
     3541 tgtttaaatc ttcatctgat gaaggctact tccctaaaat tttctcccgt gtaaccaaag
     3601 tggatgcacc ggtgcaggga atgttgacca ttgtgattat ccagagcggg ttggcgctga
     3661 tgacgattag cccgtcgcta aacagtcagt tcaacgtgct ggttaacctg gccgtggtga
     3721 ctaatatcat tccgtatatt ctgtcaatgg cggcattagt cattattcag aaggtcgcta
     3781 atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc ttttgttggc gcgatgtaca
     3841 gcttttatgc gctgtactca tccggggaag aagccatgct atacggttcc atcgtgacct
     3901 tcctcggttg gacactgtat ggtctggtct caccacgctt tgaactgaaa aataaacacg
     3961 gttaattaag caaagcaaaa aagggcgatc ttccgaccgc cctttttctt atttgatatg
     4021 tttacgcgtt tttcagaact tcgctaacaa tttcaaccgc ttctttctca atctgcttgc
     4081 gatgttcttc accaaggaag ctttcgcagt agatcttata tgcgtcttcc gtgcctgacg
     4141 gacgcgcggc gaaccagccg ttgtcagtca tcactttcag accgccgata gaagcgccat
     4201 tacccggtgc cgccgtcaga cgcgcggtga tcgggtcacc tgccagggtg ctggcgctca
     4261 ccatttccgg agacagctta gacaacgccg ctttttgtgc ggaagtcgct gctgcctgca
     4321 aacggttata gctcggcgca ccaaagcgtt ttgccagttc gttatagtgt tcctgcgggt
     4381 tcttaccggt gacagcggtg atttccgccg ccagcagaca cataatgatg ccgtctttat
     4441 cggtggacca cggcgtgccg tcgaaacgca ggaaggaagc ccctgcgctc tcttcgccgc
     4501 caaagccgaa gctgccgtcg aacagaccat caacaaacca tttgaaacct accgggactt
     4561 ctaccagctt acggcccaag tcattgacca cacggtcgat catcgcagat gaaaccagcg
     4621 ttttaccgac agcaacatct ttgccccact gcggacgatg ctggaacagg taattgatcg
     4681 ccaccgccag gtagtggttc ggattcatca aacctgccgg agtgacgata ccgtgacggt
     4741 cataatccgg gtcgttagca aacgccagat cgaacttatc acgcagtgcc agcaggcccg
     4801 ccatcgcaca ctcggaggag cagtccatac ggatcgcgcc gtctttatca aggtgcataa
     4861 agcggaaggt ttgatcgacc tgatcgttaa caatagtcag gttgaggttg taatactcgc
     4921 caatacgctt ccagtattcg ataccggaac cgcccagcgg atcaacgcca agcgtcaggc
     4981 ccgctttctg aatcgccgcc atatcaacga tatcggccag cccttccaca aacggctgca
     5041 ccagatcctg ctctttcaca tgaccggatg ccatcgcttc gtcgagggag atacgcttca
     5101 cgcctttcag gccatcagcc agcagtgcgt ttgctctgtc ttccaccact ttagtgacgt
     5161 tggtatcagc cgggccacca tttggcggat tgtacttgat accaccatct tccggcgggt
     5221 tatgggacgg tgtaatcacg ataccgtctg ccagcgggcc acctttttta ttgtgaacca
     5281 gaatggcatt ggaaatggca ggtgtcggag tgaagccatt gttttcctgc acaatgacat
     5341 caacgccgtt cgctgccagc acttccagta cggaaatgaa cgcaggctcg gacagggcgt
     5401 gagtatcttt acccacatag caagggccag tgatgccgtt tttcgcacgt tcttcagcaa
     5461 ttgcctgagc gatcgccaga atgtgcggct cgttaaagct gtggcgcgct gcactgccac
     5521 ggtgaccgga agtaccgaat ttcaccgcgt gctccgcatt ccctgcttct ggtttcagta
     5581 cataatattg cgccgtcagt tgggcgacgt taatcaaatc actctgttgt gcaggttggc
     5641 ctgcacgatt gtggattgcc attgctttgt cctttgtctg caacgtttta gatagttccg
     5701 caaaccttct caatcaattc cgccgggaat tgcatcgact gcatgatgtg ttcgatcatg
     5761 ctgcatttac ggccggtgtt ggtgttggtg atcacccaat acggcgtacc aggcacatgt
     5821 ttcggcttgg tctgattacc atttttcagc agcgtttgtt catctgccgc aaagtaaacg
     5881 cgtgtacgac cgtgcaacga ttccgttgct tcggcaaacg cctgggcgtc aagagaatat
     5941 agtgtagaca acagcagcat aaagcgattg accgctcgct tttgctctgc gtattcgtcc
     6001 gaaagcagaa gttcacgcat tgcgcgaacc ttgtctttaa tcgttttgac cggcttcgct
     6061 tcgacgatag caggtgacgc aacgcgaacc tctttcgtca ccggagcagc aggctgtgat
     6121 gcggcggtaa atttcaacat acgccgtaaa atgtcggatg cgctctcgcc gatatgctta
     6181 gtgtggctgg caatatagct gtagagttca tcatcaactt caatcgtttt catcttaatc
     6241 cagtgcagtg tcttagctga atacaagtcg ccaggagtat atgaataaat cccggcagcg
     6301 gatagcgtca agcctggctt gcagaaaaac tcggaataaa ccttatttgt tgcagccctg
     6361 tggcacaatg gtcaacataa taccctaacc cgacaacgcg aaaaaaagaa ctttgccatg
     6421 aaattgaata tccgcgcgca aactgcacaa aaccagcaca ataattctcc catcgttctt
     6481 gtccatggtc tgtttggcag cctcgacaac cttggcgtac tggctcgcga tctggtaaac
     6541 gatcacaata tcatccaggt tgatatgcgt aaccacggtc tttcaccgag agatccggta
     6601 atgaattacc cggcgatggc gcaggatctt gttgataccc tggatgcaca gcagatcgac
     6661 aaagcaacat ttatcggtca ctccatgggc ggtaaagcgg taatggctct tactgcacta
     6721 gcccctgatc gcattgacaa gctggtggcg attgatattg cgccggtcga ttatcacgtt
     6781 cgccgtcatg atgagatttt cgcggccatc aacgcagtca gtgaatcaga cgcacaaaca
     6841 cgccagcaag cagcggcaat aatgcgccag catcttaatg aagaaggggt gattcagttt
     6901 ctgttgaaat cttttgttga cggggagtgg cgctttaacg tgccagtatt gtgggatcag
     6961 tatccgcata ttgtgggttg ggagaaaatc ccggcatggg atcatcctac cctgtttatc
     7021 cctggcggca attctccgta tgttagcgag cagtaccgtg atgatttact ggcacaattt
     7081 ccacaggctc gggcgcatgt gattgcaggc gcgggtcact gggtccatgc tgaaaaaccg
     7141 gatgcggtat tacgcgctat ccgtcgctat ctcaatgatt aacgcaactg attaaaaacc
     7201 cagcgtccgc gtcgctttcc gcgcgtggac gttggcgtga ctcatcgcgc tgatgtatta
     7261 tggcgcgcta tccatccggg cagaggcctg gctgattgtt tcccccgaag tcaccaagat
     7321 catggccaaa gaacaaacgg accgtacgac attagatctg ttcgcgcacg agcgtcgacc
     7381 gggacgaccg aaaactaatc cgctttcgcg cgatgaacag ctgcgtatta ataaacgcaa
     7441 ccaactaaaa cgcgacaaag tacgtggcct taagcgtgtc gaactgaagc tgaacgcgga
     7501 agctgtcgag gcgctgaacg agctggcgga atcgcgtaat atgagccgta gcgaactgat
     7561 cgaagagatg ttaatgcagc aactggcggc gctgcgtagt cagggcatcg tttaaatttc
     7621 cactttcatg tagcacagtg tgcagtcctg ctcgtttgct gattccgcaa gactgcctgt
     7681 tctgctatga ttgcctttat ccgtgggcaa ttttccaccc ccatttcaat aagtttcaag
     7741 aggttatttc actcatggct atcactggca tctttttcgg cagcgacacc ggtaataccg
     7801 aaaatatcgc aaaaatgatt caaaaacagc ttggtaaaga cgttgccgat gtccatgaca
     7861 ttgcaaaaag cagcaaagaa gatctggaag catatgacat tctgctgctg ggcatcccaa
     7921 cctggtatta cggcgaagcg cagtgtgact gggatgactt cttcccgact ctcgaagaga
     7981 ttgattttaa tggtaaactg gttgcgctgt ttggttgtgg cgaccaggaa gattacgcag
     8041 aatacttctg cgatgcattg ggcaccatcc gcgacatcat tgaaccgcgc ggcgcaacta
     8101 tcgttggtca ctggccaacc gcgggctacc acttcgaagc gtcaaaaggt ctggctgatg
     8161 atgaccactt tgtcggtctg gctatcgacg aagatcgtca gccggaactg accgctgaac
     8221 gtgtagaaaa atgggttaaa caaatttctg aagagctgca tctcgacgaa attctcaatg
     8281 cctgatgtga tgcggcgtag actcatgtct acgccgtatt aatagataat gccaatcaaa
     8341 ataattgcta caaatttgta acttttgctg ttgtacctgt acaatgtccc ggtgttcaag
     8401 tggccttgcc gttgtaaatg taagctgtgc cacgttttta ttaacaatat ttgccaggga
     8461 cttgtagttt tcatttaggc gtggcaattc tataatgata cgcattatct caagagcaaa
     8521 ttctgtcact tcttctaatg aagtgaaccg cttagtaaca ggacagattc cgcatgactg
     8581 ataacaatac cgccctaaag aaagctggcc tgaaagtaac gcttcctcgt ttaaaaatcc
     8641 tggaagttct tcaggagccg gacaaccatc acgtcagtgc ggaagattta tacaaacgtc
     8701 tgatcgatat gggtgaagaa attggtctgg ctacggtata tcgcgtactg aaccagtttg
     8761 acgacgctgg tatcgtcacc cgccacaatt ttgaaggcgg taaatccgta tttgaactga
     8821 cacagcaaca tcaccacgat cacctgatct gcctcgactg cggtaaggtt atcgaattta
     8881 gtgatgattc catcgaagcg cgtcagcgtg aaatcgccgc aaaacatggc attcgcctga
     8941 ctaaccacag tctctatctt tacggtcact gtgccgaagg cgattgccgc gaagatgagc
     9001 acgcgcacga aggcaaataa gccagcctga acgagaaaag ccaacctgca ggttggcttt
     9061 tttatacaag ggaaataaga aaagcgagag ttacagctct cacttatttg ttattactgc
     9121 gaatttcctg ccagacctta tcacaatcgg ctttcaccgc ctgatcattt ccggtttgcg
     9181 ttgcctgaat gcactgctga taatccagta cgcggacact ttcctgctcg gcaaactgct
     9241 gatgttgttt ctctttcttc agcacgttta acacgctctg gcaggcttca attttttccg
     9301 gcgaaccttg cgcggtgttg atacaggcgc tgtaagcctc tttcagacga ctgtcttcct
     9361 gtggcgcggt tgattgcgca caagccacca gccccgatgc catcatggcg atgagaatca
     9421 gttttttcat agcaaattcc tcaaacgtgc ggcaggcaca aaacctgccg catcgggcat
     9481 cagaagatgg tgaatggtgc gattaccata aatttcacgt cacgctcatc ctggaagatg
     9541 ttgccataac caccgcccca gcttgggatg tcggagtggt tgtcgtattc ggtgaagtgc
     9601 agtttgaaca tcgtgccttt ggcgcggccg tcctgaatgg tgtagaccgc atccaggctg
     9661 taggcagact cttcaatagt ccggtttttg tcgtagtacg catccggatt gctctgccag
     9721 gtagcgggtt tagcatccca tgcgtaaacg taggaggcgc cgatggcaaa acctggaaga
     9781 ttccagtttt tcaggtcata catcgcgccg aagaagaccg ctttttcgcc gttggcgttg
     9841 aagtcagaac ggttatccca ccagatatcc aggcgaccgt tcgaggaagc gtaggttggt
     9901 gtcatacgtt gcaggaagta tccctgctga ccgtcggctt taacccaggt gccttccaga
     9961 cgcaaatcca ctacgtcagc cgcccggtaa ccaaaggtca acgcctgcag ccaggcggtg
    10021 ccgtcataaa gatcgttggc gctgcgatcg tcaactttat cgcgcgtgcc atagaactgg
    10081 tagctggtgg ttaacgggct accggcgata tcaaatttgt agctggcttt ggcaaaatat
    10141 tgatcgatat acccttcggc ctgaccaaac gccgcttcca gtacgaagtt atttttgaag
    10201 tcgtacttcg ccccaaggga gtgtagataa tcaactttgg tggttttatc gttctgataa
    10261 aactcatcca tttccagatg ccacggtgct ttgtattcgt tggtccacat gtaggagaaa
    10321 ctcaacgcac cagcatcgcc gtaatcaaaa ttcgccccgg cctccgcccc ctgataagta
    10381 cccggcataa agctccagtg tggcgctaac agcgtctgac cggttggctg aatataacct
    10441 gccctcgccc aaaccggacc gtatttaaat ttggccgctg ctttatacag gcttataccg
    10501 cttttatcgc cggaccagtc ttcgtcatag gctttattac tttttgaaaa cgcgatttcg
    10561 ttcgggtgag agctgtcgcc gttttccgcc atttcaatcg ccgtaaacgc ggcaatatca
    10621 aggccgaaca tatcagcagc ataaccagac tgaaaatcga ggttggcgtt ccaggtggag
    10681 tgagaaaggt tggttttgta tttatcgccg tcggtaacat ctttacggtc gcgttcacgc
    10741 tgccagtaat agataccgcc ggttaaggtt gaatcgtcga tgaatccttc ggcgcgagcc
    10801 tccggaattg ccataaagcc cgacatagct gtaacaccgg cgatagccag cgccagcgta
    10861 ctacgtttgc cactaaacgc acgcatgggt taatcctctt tgacgtataa attgctgcac
    10921 caaaggtgaa cagcgaaaaa aataaaaatt aaaaaagtag cgttgagaaa agtctcgctg
    10981 actacaggaa cagactattt ttcgcgacgc gaattatcaa tctctttctg taaccagaat
    11041 gtaagaacat gagcaaccgc acatattttg atgatttttg ttacaaggct taagtttgca
    11101 gttgaaaggc ttgcagaaga ataaaaacgt gaaattccag caagatgaaa gcggttacat
    11161 tttatttaat tatgttttgt gattttaaat ttattttatg gataaattgc gaagcggaat
    11221 taattcgcga agaaaagcag aaaaaaacgc cgcagagcgc ggcgtttggg ataatttgca
    11281 tatttcttgc cggatgcggc gtaaacgctc tatccgacaa cgtaaactta ctcgcctact
    11341 ttcgcccagg tatcacgcag cccaacggtg cggttaaata ccggtttttc cgccgtagaa
    11401 tggcggctgt cgaggcagaa gtaaccttca cgctcaaact ggaatgcttt acccgcaacc
    11461 gcatctttca gcgacggttc agcaaagccc tgtttgatca ccagcgattc cgggttaatc
    11521 accgacagga aatcatccgc agcaccaggg ttcggcacgc tgaacagacg gtcatacaaa
    11581 cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt gaataacacc tttgacttta
    11641 cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat aagtacagaa gatggtggtg
    11701 atattgcctt cggcatcttt ctccacgcgt tctgccttaa tgacgtaagc attacgcaga
    11761 cgcacttctt tacccagcac cagacgtttg tactgcttgt tagcttcttc gcggaaatcg
    11821 gcgcgatcga tccaaatctc accgctaaat ggcacctgac ggctgcccat ttccggttta
    11881 ttcggatggt tcggcatggt aaccatttcg ccttcgccct ggtagttttc gataaccagt
    11941 ttcaccggat cgataaccgc cattgcgcgc ggcgcatttt cgttgagatc ttcacggatg
    12001 caggattcca gtgacgccat ctcaatggtg ttgtcctgct tggtcacacc aatgcgtttg
    12061 cagaactcac gaatagaagc cgcagtgtaa ccacgacgac gcagaccaga aatggtcggc
    12121 atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca ccagcaggtt cagcttacgc
    12181 ttggacatca cagtgtattc cagattcagg cgcgagaatt cgtactggcg cgggtgaaca
    12241 ggaatggtga tgttgtccag tacccagtcg tacagacgac ggttgtcctg gaactcaagc
    12301 gtacacagag agtgcgtaat accttccagc gcatcgctga tgcagtgggt gaagtcgtac
    12361 atcgggtaga tgcaccactt gttgccagtc tggtggtgtt cagcaaactt aatgcggtac
    12421 agcaccggat cgcgcatcac gataaacggc gaagccatgt cgattttcgc acgcaggcag
    12481 gctttacctt cttcaaaacc gccggtacgc attttctcga acagcgccag gttctcttca
    12541 acgctgcgat cgcggtacgg gctgttttta cccggctgcg tcaaggtgcc gcggtattcg
    12601 cggatctgtt ccggcgtcag ttcatcaacg tacgccaggc ctttattgat cagttcgatc
    12661 gcataggcgt ggagctgatc aaaataatcg gaggagtaac ggacgttacc agaccagtga
    12721 aaacctaacc actctacgtc gtttttgatc gaatcgacat actcgatatc ttcttttacc
    12781 gggttagtgt cgtcgaaacg caggttgcac tggcctttat agtcctgggc gatcccgaag
    12841 ttcaggcaga tagatttcgc atggccaata tgcagatagc cattcggctc cggcgggaaa
    12901 cgggtgtgta ctgtggtgtg cttaccactg gccagatctt catcgatgat ctgacggata
    12961 aagttagtcg ggcgggcttc tgcctcactc atcgtggatt cctcaaagcg taaacaacgt
    13021 ataacggcgt atgatcttat aagcgggaca ggctgacaac tgttagttag cgaaaaaatg
    13081 gttttgcgta agatattggg gcgttttctg cgctactgcc agcagatcct cgcgctggaa
    13141 acgtaaggcc aaacgtcgcc agcctgacgc atttattaaa tttccggatg agctggccgc
    13201 agagtcgtag attagtcggc tatgattaaa cgcgtcgaca aggacgcaag cgttctacgc
    13261 gtctgcccat caagctgact atccgtaata atctctgtca gttcattaag atgcgcgata
    13321 ttaaacagcg accatgaacc gtattttgag ctgtcagcca ggagaatttt gcgcgaagca
    13381 tgcgcgataa gatcgcgttt tagtgcggct ttctcttccg tgggtgctgt aatacccttt
    13441 tcaagatccc agctgttaca gcttaaaaat gccacgtccg gccaggtatt ttgcagcaat
    13501 ttacgaccat gttcgccaat gcaggactgg ctggagtcgt caatacgtcc gccgataatc
    13561 gtcacttcaa tttgcttgaa ttcagccaaa aaaagcgcga tatgcaggtc gcttgtgatc
    13621 acgcgaagcg gtaaatgtgt aatctgccgc gcaagttcaa tcattgtcgt accggcatca
    13681 aggacgacgg aatcccccgc tttgaccatt gacgccgctg cgctggcgat ggcatgtttt
    13741 tccgccaggc tgcgctgcat tttctcatgg gtagtcggtt gggtaggaat aaaccggttg
    13801 agagtcacac cgccgtgtgt acggctgata acaccctgct catcgagttt tatcaggtct
    13861 cgacgaatcg tagccggcga cgcattggtt accgccacca gctcatctac ggtgaccaga
    13921 ttatggcttt tcaggtaatc catgatctgc tctaaccggt tatatccctt cattttattc
    13981 tcgcgaaatc tgcattgcta actggatgga aactgccatg ctctcagact tggcttttcc
    14041 tgtccaggca atatcaaacg cggtgccgtg gtcggcagaa gtgcgaataa atggcaaccc
    14101 ggcggtgata ttcaccccat cataaaatcc aagcagtttt aaagggatat gcccctgatc
    14161 gtggtacata gccactacca tatcgaatag cccttcatgg cattgcataa acacggtatc
    14221 tggcgggcac gggccagtaa catcaatatc ttcggcctgc attgcttcaa tcgcaggcgc
    14281 gataatctcg atttcctcag tgccgaataa accatgttcc cctgcatgtg gatttacacc
    14341 cgccacggca atacgcggac gctcaatacc aacacgtttc aggaagtggt tcgcaactcg
    14401 aataaccgtt ttgacccttt caccattcaa agtatccagg aatttacgca gtgagatgtg
    14461 agtcgaaata tggataacct tgagcttctc ggtatagagc accatcgcgt actctttgct
    14521 gccggtaaga tgggccagca attccgtatg ccccggatag ttatgcccgc caagatgcag
    14581 cgcttcttta ttcagcggcg cagtggctat ggctttcacc tcgcctgata aggccagagc
    14641 cgttgcacgt ttgatacagc gatacgctaa atcgcctgcc acagcctgta ccactccggg
    14701 tgtcagcgca tcggggttag ccagcggctc atccatcacg ttaatcacgc cgggagcaaa
    14761 atgggcgtcg cacacctttt gaatgacgcg taactctgcc tgcggcgtga tattcatcgc
    14821 cataatacgt cgcagcgtgc gcacacagcc caccaccacc accggggcac cagcaagccc
    14881 ggcttcgctc agggatttaa taatgatctc cgggccaata cccgccggat cgcccatcgt
    14941 taccgcaata atattattca ctacgcttct cctcgataaa attcagaacc tgatgcaacg
    15001 tggtctcatc gccaaaccca ccggctttcg tcatcaccga acgggaccag acgccgccca
    15061 gaaagtgacc gtatggaacg cattgcgcca ccttccccgt aatacgaaat ccggtcgcgc
    15121 ctaacgcact ggctgttgcc attgcgacat caccaccaga gagataaagc gcatcgggca
    15181 ttgcgcaaag cagaacttgc cgggtaagtt cgccaagaaa ccggcaaatt ttttccccaa
    15241 gtgcagcgcg actcatctgc caacgctggc aaagggtgtc gatctgatgc cttgctacag
    15301 aatcgttaca ggtgtggaca atacagtggt cgcctttctg caaggcctgc acaatacgcg
    15361 cgtcatagtc actggcattg cctgccagaa tgtgttcaac atcgacgtat atctgtgtga
    15421 tacgtgggtg tgaatgaagt gtggcgatct gccgttgagc gatttcgctc attgaaccaa
    15481 taatggccag cacggtgggt ggcggcataa tcgcggagcg ccgggcaacg gcatcacaaa
    15541 taccagcgga gccgaccagc agcagcggtt gtttagcctg catcacagcg gcagcgatgc
    15601 gatccaggtc gctgtccgtc tgcgcatcta tcacgccaat ccacggttgc tcataagaca
    15661 aatggctgag ctgagaaacc gtaacgggct ggcatggaat ggccgtctgc aaccggatga
    15721 tgtccgcaat tcgggcgctc aataccgggg ttttcggatc gctagcaaac tcggtttccg
    15781 ttatccgcac accgtttacc caacactcac cggccactgt ggtccttccc gcctgaggaa
    15841 atgcgggggc aaccactgcg cccgttatac cgcatgcctt catcagggct tcaatttctg
    15901 cgccgatatt gcctcgcagc gtggaatcta tctttttaat cacgagcggc gggttattcg
    15961 ccagagggag atgactcatc agcgaggtta ttttcatcgc cgccagctta ggatccatcg
    16021 cccgactgtc gctgttaatc acccatacgc tggcatcacc gcgataaggc atttcaaagg
    16081 ccacatcaac tgtatgcccc acctgcgcca ggcttacccc cgcgtcgttg gctcccgtga
    16141 aatcatcggc aacgatgaga atgctcggct tagtgattcc cttttccatc atcactccag
    16201 gttgttttat gataacaatg attatattca atcatgattg attaaatgtg agcaagctca
    16261 agttattaat cactcgaata aattataaaa ttagaccatg ttacgtaggc tacggcattt
    16321 tgaatgattg aattcaatca ccagaagaga ggttatttgt ggtaattatc aatagcacga
    16381 tactgagcgg cgcaggcgct atcccttccc tgacgtcgct cttacccgac atcagaaaaa
    16441 tgctgctggt cactgaccgt aatattgcgc agctcgacgg tgtgcagcag attcgcgcct
    16501 tactggaaaa gcactgcccg caggttaacg ttatcgataa tgtgcccgca gagcccacgc
    16561 atcatgatgt gcgccagcta atggatgccc ttggcgatgc ctcttttgat gtggtggtcg
    16621 ggatcggcgg tggcagcgtg ttggatgtgg cgaagctgct atcggtgctt tgccatccac
    16681 aatcaccggg gctggatgcg ctgcttgcgg gtgaaaaacc gactcagcgg gtgcaatcat
    16741 ggttgattcc tacaaccgcc ggaaccggct cagaagccac gccgaatgcg attctggcaa
    16801 tccctgagca aagcacgaag gtgggtatta tttcccaggt gctgttacca gactatgtgg
    16861 cgcttttccc ggaactgacc accagtatgc ccgcgcatat tgcggcgtcc acgggcattg
    16921 atgctctttg ccacttactg gagtgtttta ccgcgaccgt ggcaaatccg gtcagcgata
    16981 acgcggcgct gactgggtta agtaaacttt tccggcacat tcaacccgcc gtgaacgatc
    17041 ctcaggatct gcgcgcaaaa ctggaaatgc tgtgggcgtc ttactatggc ggcgtagcga
    17101 taacccatgc gggcacgcat ctcgttcatg cgctctccta cccgttaggt ggcaaatatc
    17161 atctgccgca tggcgtcgcg aatgccatct tgctggcgcc gtgcatggcg tttgttcgcc
    17221 cctgggcggt cgagaaattt gcccaggtct gggattgcat tcccgatgcg gaaaccgccc
    17281 tgagcgcgga agaaaaatct catgccctgg tgacctggtt acaggcatta gtcaatcaac
    17341 tcaagctacc caacaatctc gcggatctcg gcgtaccgcc agaggatatt gcctctctga
    17401 gcgaggcggc actgaacgtg aagcgcctta tgaacaatgt gccgtgccag cttgatctac
    17461 aggacgtaca ggccatttac caaacactgt ttccgcaaca tcaatttaag gaataaatca
    17521 tgccgaaaac cattgaaggt gtattaaccg ctatcgtcac cccgtttacc gcgaccggca
    17581 gtctgaatat cccggcgtta aaagtgcagg tgaatcgtca actggaagcg ggtaacgcga
    17641 ttttttgcgg cggcaccaac ggcgagtttt ttgtgttgaa tgaacaggag aagctcagcg
    17701 ttacgcaaac ctgcgtggat gaagtggctg gtcgtgcgcc agtcgtggca cacattggcg
    17761 agatctctac gcgcgaaacg attcgtctgg gtcagcagat tgaaaaactt ggcgtagatg
    17821 ccgtgtcggt cattgcccct tacttcgtac cgctgaaaca ggaagagttg attgcgcatt
    17881 acagcgcaat tgccgacaca ctgagcattc cgatgtttct ctataacatc ccggcccgca
    17941 ctggcaatac gatccagcca gaaaccgcgc gtgctctggc gtcgcacccc aatatcattg
    18001 ggattaaaga cagcgacgga agcgacgaaa gcctgaaggg ttttctcgat gcggtgcgcg
    18061 acattgacgg ctttaatgtg ctcaacggcc cggattcgct gatccataaa gggtttgtcg
    18121 agggatgttc agcctgtatc tctgggctcg ccaacgtcgc gccgcgagaa atcaatgcca
    18181 tctggtctcg cttccatgcc ggtgatattg aagggtctta tgaagcacag gagagtgtaa
    18241 cgggcctgcg taccgatctt tataaagtag cattctcccc ggctgcggtg aaaaaagcgt
    18301 tgcagcttat ggggcatgat gttggtgata gccgctacgc ggttaatttc acgcctgaac
    18361 agatcagcga gattcaacaa attattcgtc attataacat taattaaatt aactctatta
    18421 tccagcccgc tataaagttt agataaagag aataaaggat taccccatgt ttttgtggtt
    18481 tatgcagcca gatatctggc tcactttaat taccctcacc tttctggaaa ttattcttgg
    18541 ggtggataat attatttttc tttcactggt ggtggcaaaa cttccgttat cacaacaaaa
    18601 tatggcgcgt aaactgggtt taagctgcgc gatgattatg cggatattat tgctgatatc
    18661 cattgcgtgg ctttcacata ttacccagcc gctttttacc gtgctgacgc tggagatttc
    18721 gttccgcacg ttgattctgc tcgggggcgg gctgttcctg atttataaag cgatacagga
    18781 gatacgcgaa gagctgtcgc ccgcgcagga agacgaacat ggcagcggat cgcggcaact
    18841 gtcgttgtgc ggtgccattg tacagataat gctgctcgat atcgtgttta gcctggattc
    18901 ggtgatcacc gccgtcggcc tttctcagca tatatttatc atgattgccg ccgtaatgat
    18961 tgccgtcggt gtcatgatgt ttgcggcgaa aacaattggt gatttcgtta acgccacgcc
    19021 gtcaataaaa atactggcgt taaccttttt attgtttgtc ggtgtattgc tggttgccga
    19081 tagtctcaac attcatattg ccaaagagta tctctacttc gcgatatttt tctcattaag
    19141 tgttgagaca ctcaatatta tccgtgaaag aaaaaataaa aaagtttcga cattgatcga
    19201 aacgcatgaa tcgcactcgc attaataaat ctattaccaa ctttaagatg gagtctaact
    19261 atgtctgtag cccttattcg tgacggcgtc atccgtccat ccgaaaacga ttcgcatgtg
    19321 cttaccgcca tgctgccctc gtcctgcccg caaaaccacg cggcgaatat tctgcctttg
    19381 ccggatggcg cgttaatgtg cgtctggttt gcgggaacgc aggaaggtat tgccgatatt
    19441 tctgtctggg gatcgcgatt gcccgctggc ggaatgcagt ggagcgatgc ggccaaactt
    19501 tcccacgatg acacgcgttc agagcaaaac ccggtgctgt ttctcgcgcc ggataacgtg
    19561 ctgtggctcc tatggaccgc acaaatatcc ggtaatcagg acaccgccat cgtccgctac
    19621 cgtaagtcag acgacctcgg acaaacctgg ggcgagattg ccaccctgct tgataaaccc
    19681 ggtacgttca ttcgacagcc aatcacggtg cttgataacg gcaactggct gctgcccgtc
    19741 ttttattgcc gtacccaacc cggcgaaaaa tgggtcggca atgatgatat cagcgcagtg
    19801 aaaatttccg ctgacggtgg tcactcctgg cgcgatgtcg aggtgccgca aagtctcggc
    19861 tgtgttcata tgaatatcac aatgttgcac gacggtacgt tggttgctct ctttcgcagc
    19921 cgttgggcag acaacatcta tatcagccat tcagtggaca acggcgagag ttggtcggtg
    19981 ccgcaagcaa ccgaattgcc caacaacaat tcgtctattc aggtaacgac gcttgccagc
    20041 ggcgaactgg cgctggtgta caacgcgatg agtgcggcag gcgcagtaga acgtcgcgcg
    20101 tcgctttatg acgaaatcga cgatggcgac gacagccgca aagaaccgac agccgtcgga
    20161 cgctccgctt tctggggtgc cccacgcgcg ccgatgacgg tagcgatttc tgccgatggt
    20221 gggaaaagct ggtcgtggcg gcgcaatctc gatgaaggcg acggctactg catgaccaac
    20281 aattccctgg aaaagctaaa tcgtgagttc tcctacccca gcatcaaaca aagcccggac
    20341 ggtacgctac atatcgctta cacctggtgg cgtcaggcca tcaagtacgt acgcatctcc
    20401 cccgagtggg ttaaggggca ggcatcatga ttatcggtaa tctcaaccat ttgtcgctgg
    20461 cggggcttcc tgcctgggtg agaaatattc tgctgcgccc ggagtgttcg ttatcggcgt
    20521 tatccaccag agaggacggt cgctggcaac ctgaaggatg tcggtggttc tgctcgctgg
    20581 gaacttcaga cacgcaaccc gccgaacttc gccatacgga gtatcaccat ctatgggccg
    20641 atatacaggt ggtgattact ggttgtgagg gcattaatgc gggaacgcgg cccatcgctc
    20701 gcgaaaacga tgaagaacgt aagcccgatc tgtttatcgc cccatcccct gaaaacagtg
    20761 tcgccatcac cctacacgcc ggtgatttcg ccgtctttat gcctgaggaa cctcaccagg
    20821 cactgtgcgc aattggcacg gctacttcaa tacgcaaagc ggtctttaaa gtaccgcgcg
    20881 acatgctgga agcctaaaag gagagaaaaa atgacaatgg aaatgatcct ggcattagga
    20941 atactggtcc tgatgatcgt gctgatcatg tcggacaaaa tgccttttgg cgcaccgcca
    21001 ctgcttgcgt gtttattgct ggttgtatcg ggactttcga ccgttcaaca ggcttttgcc
    21061 ggattcgtca atccaagcgt ggtgatgatt gcagggttta tggtggtcat ggcggcgcta
    21121 caaaaaaccc ggcttatcag caacgttaaa tctgcgatga taagcctggt caataaaggc
    21181 agttaccgca gctatgggct gttgttagtc attgttatgc tgggtgccag tcttgccggc
    21241 accggagcga ccggttatta cgtgctaatt ctgtcgctgg tatccaccat tccgtacagt
    21301 aaaaagctac caacatcgaa attaatgatg cccctgggat ttgccaccaa ccacccgctt
    21361 cttcctatca atctcgcgct tctctttggc gttacggcga ccgtactcga aaccgcgggg
    21421 tttcatcagg cgatttcgat ggggcgcttt gcgctggtga atctcatcat gtcagcggct
    21481 ttcctcgcat ggagtcttat tgcctaccgt tttcttccgg atcatcccat tgcggatgct
    21541 tccgaggatg cgctggcggc acgcgaggaa acgtttaacg cccttcctgc ctggaaagaa
    21601 tattgcacta ttgccgcatt cgctgtcagc gttatcggca tgatgctaat gaacattctg
    21661 ggaaatatcg ccttcgttat tccgggtctt gcgggtgcgt ttgttctgat gatcgatgta
    21721 ctggacttca aagaagttcg cgatcatatg ggggctccgg tcattttaat gatggcgggg
    21781 gtgatcggtg tagctgatgc actggcgggc accggcttta cggcgatggt tggcgatgcc
    21841 gttgcaggtg tgctcggttc tggcgtctca ccgtttgtat ttattgtcgc ctttgctttg
    21901 cttaccagta cctgcgccac cttcacgggt tcgaatatgg ggtcagtata tatttttgcc
    21961 cccatcgcca ttgccgcctg tactagcctt gggcttaacc cgaccgctgc cgcgattgcg
    22021 gtggtgattt ctggctggaa tggcggctat atgcctattg acggaatgcc agccatgatc
    22081 ctcggaatgg ggaaatacaa acttcccgaa ttctgggtgt tctctgtacc aatgtatctc
    22141 atccgcattc tggcgctttg tgcaggcgct gtttttatct tcccaatgta atctcagaca
    22201 aaaaaagcgg cagagaagat ccctgccgct ttttattgtt ggatgcgacg ctgccgcgtc
    22261 gcatcagaca taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa
    22321 catggccctg agctttaatg ataaggccgc tgaaatcatc gatattgctg caaaccaccg
    22381 ggctaatcat cgaacgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt
    22441 gccctgcgct tacctgcgcg ccctcttcca ccagacgttt aaagccttta ccttccagcg
    22501 ctacggtgtc gatacccata tggacgacga tctccgcgcc tttttcggtt tccagacaga
    22561 acgcgtggtt ggtgttgaag attttcacga ttgttcctgc ggctggtgat acgacgattt
    22621 tatctgtcgg tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag
    22681 gaatctgatc cagtgccaca acatccccgg taatcggcga taccagctcc gcgatagata
    22741 ccgcgtttgg tacagcctgc ggttttgcta caggagcggc agttgccgga gttgcttcag
    22801 ctgacgcagc ggctaccgga ccacgggcaa cgactttctt catcgcatcg ccgatggatt
    22861 ctgctttcgc gccaacaatc acctgaatag tctgtttgtt cagttttact accccagaag
    22921 cacccagacg tttacacatc gcatcgttga cgcgagctga gtcaaccacg gtaaggcgca
    22981 gacgagtaat acaggcgtca atggctttca ggttgtcagt accgccaacc gcagcaatat
    23041 agttggttgc cagttgattg agaccttctt cagtattgct gttagcctct tcagtaacga
    23101 tctcgtcttc tttatcttca cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac
    23161 tgaacaccac gaagtagata gcgaagaaga caacccccat caccagcagc atccagacgt
    23221 tctggctggc ggccggcagg ttatacatca acgcgtagtc gatagccccc gcagagaaag
    23281 agaagccggc atggataccc agcaacgttg ccacaaacag gctgataccg gtcagcagtg
    23341 cgtgcaggag gtacagcagc ggtgcaagga acatgaacag gaattccagc ggctcagtca
    23401 caccggtcag gaacgcagta acagcaacag aaagcagcat cccgccaacc atcggacgac
    23461 gctctttcgg tgctgcgaag tacatcgcca gcgccgcacc cggcaggccg aacatcataa
    23521 tcgggaagaa gccggacatg aacatccccg cggtgccgtc accggcatag aagcggttga
    23581 tatcaccgtg gaaaaccgtt cccgccgcgt tggtgaattc accaatctgg aaccaggcga
    23641 tggtgttcag cacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga
    23701 taccggaacc cagcgcgccc gcagaaacga tccactcgcc gcctgcatgg atagcgtgct
    23761 gtaccggtgg ccagacgtaa ccaaaaatgg ccgccagcac cagacagaag aagccggtgg
    23821 ctatcggcac aaagcgtttg ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg
    23881 accaacggtt ataggctgcg ccaccaacca gaccggtaat gatacccgcc agtacaccca
    23941 tgttaatttc tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac
    24001 ccgccagtgc cgccgcacct gcgctgtctt tcgaccagct ggatgccacg ccgatggcga
    24061 atattaatgc gaggttatca aaaatcgcac cgcccgcctg ggcaataaac gcaacgttaa
    24121 gtaaatctgg ctgaccgaat cgcagcaaca gcgccgccac cggcagcacc gcgataggga
    24181 gctgtaacgc cctaccgagt cgctggaaaa aacctaaaat attcatctta ttccccctac
    24241 gagaacccta tttggctcgt ttaaagccgt atttttattt tgctgcaaat tgtactgccg
    24301 atgttctgta atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgta
    24361 tcgcaaatta aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc
    24421 cttttactgg ctaaaccaga aaacttattt tatcattcaa aaaatcaggt cggattgacg
    24481 cctgtctgcg caaatccagg ttacgcttaa agatgcctaa tccgccaacg gcttacattt
    24541 tacttattga ggtgaataat gagactgatc cccctgacta ccgctgaaca ggtcggcaaa
    24601 tgggctgctc gccatatcgt caatcgtatc aatgcgttca aaccgactgc cgatcgtccg
    24661 tttgtactgg gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa
    24721 atgcataaag caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat
    24781 gtcggtctgc cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc
    24841 gatcacgttg atattccagc agaaaatatc aaccttctca acggcaacgc cccggatatc
    24901 gacgccgaat gccgccagta tgaagaaaaa atccgttctt acggaaaaat tcatctgttt
    24961 atgggcggtg taggtaacga cggtcatatt gcatttaacg aaccggcgtc ttctctggct
    25021 tctcgtactc gtatcaaaac cctgactcat gacactcgcg tcgcgaactc tcgtttcttt
    25081 gataacgatg ttaatcaggt gccaaaatat gccctgactg tcggtgttgg tacattgctg
    25141 gatgccgaag aagtgatgat tctggtgctg ggtagtcaga aagcactggc actgcaggcc
    25201 gccgttgaag gttgcgtgaa ccacatgtgg accatcagtt gtctgcaact gcatccaaaa
    25261 gcgatcatgg tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat
    25321 ttcaatgaat tagaagcaga aaatatcaag ggtctgtaat tgttatccct gccctggctc
    25381 cttgctcagg gcaatatttt ttaaaatcgg gggtcagaat gtatgcatta acccagggcc
    25441 ggatctttac cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga
    25501 ttaaaagcgt ctgtccggta gcggaactgc cgccagagat cgaacaacgt tcactgaacg
    25561 gggccattct ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt
    25621 ttaacgacac cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg
    25681 agaaatcagg ctgtactaac tatctgccga cgcttatcac caccagcgat gagctgatga
    25741 aacagggcgt gcgcgttatg cgcgagtacc tggcaaaaca tccgaaccag gcgttaggtc
    25801 tgcatctgga aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg
    25861 tgcgtaagcc tgatgccgcg ctggtcgatt tcctgtgtga gaacgccgac gtgattacca
    25921 aagtgactct ggcaccggaa atggttccag cggaagtcat cagcaaactg gcaaatgccg
    25981 ggattgtggt ttctgctggt cactccaacg cgacgttgaa agaagcgaaa gccggtttcc
    26041 gcgcggggat tacctttgcc acccatctgt acaacgcgat gccgtatatt accggtcgtg
    26101 aaccgggcct ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attatcgctg
    26161 atggcctgca tgttgattac gccaacattc gtaacgctaa acgtctgaaa ggcgacaaat
    26221 tgtgtctggt taccgatgcc accgcgccag caggtgccaa cattgaacag ttcatttttg
    26281 cgggtaaaac aatatactac cgtaacggac tttgtgtgga tgagaacggt acgttaagcg
    26341 gttcatcctt aaccatgatt gaaggcgtgc gtaatctggt cgaacattgt ggtatcgcac
    26401 tggatgaagt gctgcgtatg gcgacgctct atccggcacg tgcgattggc gttgagaaac
    26461 gtctcggcac gctcgccgca ggtaaagtag ccaacctgac tgcattcaca cctgatttta
    26521 aaatcaccag gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac
    26581 accaggcgga caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc
    26641 ggtttatcgc ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag
    26701 tcagcttgcc cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat
    26761 caaagaagtt gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga
    26821 aacccgcaat ttccacgcaa tcggcgtacg gcttggtcgt cacgacgcca ccatcacctt
    26881 gtttgatctc agcagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca
    26941 gcagacgctg gaacatgccc tgctgaatgc cattgctcag tttattgata gctaccagcg
    27001 caagctacgc gagctgatcg cgatttcggt gatcctgcca gggcttgtag acccggacag
    27061 cggcaaaatt cattacatgc cgcatattca ggtagaaaac tgggggctgg ttgaagcact
    27121 ggaagagcgt tttaaagtga cctgttttgt cggccacgat atccgcagtc tggcgctggc
    27181 agagcactac ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtccatcg
    27241 cggaaccggg gccgggatta tctctaacgg gcgaattttt atcgggcgca acggcaacgt
    27301 cggtgaaatt ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt
    27361 tggctgcctg gaaactatcg ccgccaatgc tgccattgaa caacgggtgt tgaatctgtt
    27421 aaagcagggc taccagagcc gtgtgccact ggacgactgc accatcaaaa ctatctgcaa
    27481 agccgcgaac aaaggcgata gcctggcctc ggaagtgatt gaatatgtcg gtcgtcatct
    27541 gggtaaaacc atcgccattg ctatcaactt atttaatccg caaaaaattg ttattgctgg
    27601 agaaatcacc gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca
    27661 ggcgctgaag gcgtttcgta ctaatctacc ggtggtacgt tctgagttgg atcaccgctc
    27721 ggcaatcggc gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca
    27781 tttgctggaa aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag
    27841 tccatgacca ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc
    27901 gccgtaccgg gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg
    27961 ttgctgacca actatccttc gcagaccggg caagatctgg cgaatcgctt tgccactgcc
    28021 ggtgtcgatg taccggatag cgtgttttat acctccgcaa tggcgaccgc cgattttctg
    28081 cgtcgccagg aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg
    28141 tacaaagccg gtttcactat taccgatgtg aaccctgatt ttgtgattgt tggcgaaacg
    28201 cgttcctaca actgggacat gatgcataaa gcagcctatt tcgtcgctaa cggtgcacgc
    28261 tttatcgcca ccaacccgga cacccacggg cgcggttttt atcccgcctg tggcgcgttg
    28321 tgtgcaggga tcgagaaaat ctccgggcgc aaaccgttct atgttggaaa gccgagtccg
    28381 tggatcatcc gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac ggtgattgtc
    28441 ggcgataact tgcgtaccga tattctggcg ggcttccagg ccggtctgga gacgattctg
    28501 gtgctttctg gtgtttcgtc gctcgacgat atcgacagta tgccgttccg cccaagttgg
    28561 atttacccat cggtcgctga aatcgacgtt atctgaatag caaccacgcc tcagggcgtg
    28621 gtttcttatt tttacccatc attaaaatca ccccattcaa taaaacccgt caaaaactat
    28681 cgatccatca ataaatatac ccaaaagccc tccccttttt catcaatcag caatcgccat
    28741 tacgtttttt atttttccac tgccaaatcg cttgcgcacc gtgctgcttt gcggcatttt
    28801 tttaacaagt aaacacaaca cgcagtaaac accaggttaa tggagaaggt tatgtgttca
    28861 atttttggcg tattcgatat caaaacagac gcagttgagc tgcgtaagaa agcactcgag
    28921 ctgtcacgcc tgatgcgtca tcgtggcccg gactggtccg gtatttatgc cagcgataac
    28981 gccattctcg cccacgaacg tctgtcaatt gttgacgtta acgcgggggc gcaacctctc
    29041 tacaaccaac aaaaaactca tgtgctggca gtaaacggtg aaatctacaa ccaccaggca
    29101 ttgcgcgctg aatatggcga tcgttaccag ttccagaccg ggtctgactg tgaagtgatc
    29161 ctcgcgctgt atcaggaaaa agggccggaa tttctcgacg acttgcaggg catgtttgcc
    29221 tttgccctgt acgacagcga aaaagatgca tatctgattg gtcgcgacca tctggggatc
    29281 atcccactgt atatgggcta tgacgaacac ggtcagctgt atgtggcctc agaaatgaaa
    29341 gccctcgtgc cagtttgccg cacgattaaa gagtttccgg cggggagcta tttgtggagt
    29401 caggacggcg aaatccgttc ttattatcat cgcgactggt tcgactacga tgcggtgaaa
    29461 gataacgtga ccgacaaaaa cgagctgcgt caggcactgg aagattccgt taaaagccat
    29521 ctgatgtctg atgtgcctta cggtgtgctg ctttctggtg gtctggattc ctcaattatt
    29581 tccgctatca ccaagaaata cgccgcccgt cgcgtggaag atcaggaacg ctctgaagcc
    29641 tggtggccgc agttgcactc ctttgctgta ggtctgccgg gttcaccgga tctgaaagca
    29701 gcccaggaag tggcaaacca tctgggcacg gtgcatcacg aaattcactt cactgtacag
    29761 gaaggtctgg atgccatccg cgacgtgatt taccacatcg aaacttatga tgtgaccact
    29821 attcgcgctt caacaccgat gtatttaatg tcgcgtaaga tcaaggcgat gggcattaaa
    29881 atggtgctgt ccggtgaagg ttctgacgaa gtgtttggcg gttatctcta cttccacaaa
    29941 gcgccgaacg ctaaagagtt gcatgaagag acggtgcgta aactgctggc cctgcatatg
    30001 tatgactgcg cgcgtgccaa caaagcgatg tcagcctggg gcgtggaagc acgcgttccg
    30061 ttcctcgaca aaaaattcct tgatgtggcg atgcgcatta acccacagga taaaatgtgc
    30121 ggtaacggca aaatggaaaa acacatcctg cgtgaatgtt ttgaagcgta tctgcctgca
    30181 agcgtggcct ggcggcaaaa agagcagttc tccgatggcg tcggttacag ttggatcgac
    30241 accctgaaag aagtggcagc gcagcaggtt tctgatcaac agctggaaac tgcccgcttc
    30301 cgcttcccgt acaacacgcc gacctcaaaa gaagcgtatc tgtaccggga gatctttgaa
    30361 gaattgttcc cgcttccgag cgccgctgag tgcgtgccgg gcggtccttc cgtcgcttgt
    30421 tcttccgcta aagcgattga gtgggatgaa gcgttcaaga aaatggacga tccgtctggt
    30481 cgcgcggttg gcgttcacca gtcggcatat aaataagacg tgtaatattg ccccggagaa
    30541 atccggggca atttcagtta ctggggcgct tgttgattct gatatgcagt aaggaaatgt
    30601 tccgggtttg ccacatcgct atttgacaac tcatcactgt aggccaccaa ccaggtcgcc
    30661 tgctcatccg gaatatgcag tctgccattt ccctctttca ggcgtaataa ctcaaaagat
    30721 ttgcccttaa ttacggcggt ataggatttc tcaccttcct gtaatgcttt cactttacag
    30781 cgaaatatct gaccatcgat gtcattatca acatgcgata cctgaatgag ggcttcatta
    30841 gaagctttcg ggcctatccg catcatccac accacagcgc cttcctgccc acgatagcca
    30901 aaaacatatt taccgatttc cggtttaacc gtatcggcga acgcagattg cataagtaat
    30961 aacgcaccca tcaacgccgt cataaaacgc agcatagtat tccctcaata aatcgcctga
    31021 agtggtagca gagatgcagg cgtagcgatc cctgccctgt cctgttgcaa cgggccagta
    31081 tttcatagat taatttccca ccactattgt tgaattccga atttcatcct gtatgacaaa
    31141 agaatggctg tagggtgggg aaaaaacaca aagtaaacaa taattgacga atatagcgcc
    31201 atgctgttcg caacctaacc aaacagtcac tttcgagcaa ttttccttga aaaagaggtt
    31261 gacgctgcaa ggctctatac gcataatgcg ccccgcaacg ccgataaggt atcgcgaaaa
    31321 aaaaagatgg ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca
    31381 ggttcgaatc ccgtcgtagc caccatcttt tttttgcggg agtggcgaaa ttggtagacg
    31441 caccagattt aggttctggc gccgcaaggt gtgcgagttc aagtctcgcc tcccgcacca
    31501 ttcaccagaa agcgttgtac ggatggggta tcgccaagcg gtaaggcacc ggtttttgat
    31561 accggcattc cctggttcga atccaggtac cccagccata ttcttcgagt aagcggttca
    31621 ccgcccggtt attggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc
    31681 ctggttcgaa tccaggtacc ccagccatcg aagaaacaat ctggctacgt agctcagttg
    31741 gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccaa
    31801 attctgaatg tgtcgaatat gttcggcaaa ttcaaaaacc aatttgttgg ggtatcgcca
    31861 agcggtaagg caccggattc tgattccggc attccgaggt tcgaatcctc gtaccccagc
    31921 caatttattc aagacgctta ccttgtaagt gcacccagtt ggggtatcgc caagcggtaa
    31981 ggcaccggat tctgattccg gcattccgag gttcgaatcc tcgtacccca gccacattaa
    32041 aaaagctcgc ttcggcgagc tttttgcttt tctgcgtata ttcaatgccg aatacgatgt
    32101 tgactcgtct tatccttaca aagtcggatg cgacgctgcc gcgtcttatc cgacctacgg
    32161 ttggcatgca tccagcaatg ttgtgaggct acaaccctaa cgcatatttc agcgccttac
    32221 gtttcaacac gccagcacgc tccgccgcca tcaaaccaag attacgcaca aaacgcagcg
    32281 gtggcagatt attgctgaat ccggcataaa acagatccat accactttgc atcatgaagt
    32341 tatccgccat acgccgcatc tggtaacgct tgagcacagg ataactggcc cacgcttcgc
    32401 cgtagctgcg ggcgttcacc agaacgtcaa tcagggcatc gacatcacga taaccaagat
    32461 tcactccctg ccccgccagc ggatggatgg tatgcgcggc atcgcccacc agagcaagcc
    32521 ctggttgcac atactgcaac gcatggcgac gtataagcgg aaacgcacca gcggcaagcg
    32581 gtgtcacgta acccagacgc gacgggaaat gcttcgcgat ttccgcctgt agctgcgcca
    32641 tattcatatt ctgcaactgg cgaatgcgcg ccggagagtc ataccacacc agcgatgccc
    32701 agttatcaaa caacggcaga aacgcacgcg gtccgtccgg ggtaaattgc tgccaggtgc
    32761 tgtcgccggg atcgttctca cactgtacgc taatcaacat acacgactgc gcatactgcc
    32821 aggcatgaac gccaattccc gccatctgtc gcacctgcga atttgcgccg tcggcaccaa
    32881 tcaccagctt cgcgaggatc gtttcaccgc ccttcagttc cagctcctga agatcattat
    32941 ggcgatgcag tgcagtcagc gaggttggca cacgtaacgt tactttcgga tgcgcttcca
    33001 gcgcctgcca caaagcctgt tgcaggacag tgttttccac catgtagcca agcaacggta
    33061 gcttaagttc agcggcgtca aacacgacat gcgccgtttc ccactcccac gtttccagtc
    33121 tgcggtaagg atggcaacgc atagcctgta ctgcatccca gacccctaac cctttaagca
    33181 atgataccga agccgcgctg atcgccgaga tccgcacatc cggttggctg tcagcgacaa
    33241 acggcgcagg ttctgcgtgc tcgatcaccg ttaccgtaaa cccgtgctgt gccagcccca
    33301 gcgccagtgc gccgccgacc attcctccgc cgacaatggc aatttccgtt ggttgatttg
    33361 tcatggtcaa tcatcctgtt agcaatatgc tttaagttta caggattttt acccccaagg
    33421 gctgataacg ctcatactgg tcacaacggc agcaaagcat tacactatgc gccctgcatt
    33481 cctggctact atttcgcaag agcaagtcga tgaccaaaaa actccatatt aaaacctggg
    33541 gctgtcagat gaacgagtac gattcatcga agatggccga tctgctggat gccacccacg
    33601 gctatcaact gaccgacgtg gcggaagaag cggatgtgct gctgctgaac acctgctcaa
    33661 tccgcgagaa ggctcaggaa aaagtcttcc atcagttggg tcgctggaaa ctgttaaaag
    33721 agaagaatcc agacctgatt atcggcgtcg gcggctgcgt ggcatcgcaa gaaggcgagc
    33781 acattcgcca gcgcgcccac tatgtcgata ttatttttgg gccgcaaacg ctgcatcgtc
    33841 taccggagat gatcaactcc gtgcgcggcg accgcagccc ggttgtagat atcagcttcc
    33901 cggaaatcga gaagtttgac cgtctgccgg aaccgcgcgc cgaagggccg accgcgtttg
    33961 tctccatcat ggaaggctgc aataaatatt gcacctactg cgtggtgcct tacacccgtg
    34021 gtgaagaggt aagccgtccg tccgacgata ttctgtttga gatcgcccag cttgcggctc
    34081 agggcgtgcg tgaagtcaac ctgctcggtc agaatgtgaa tgcctggcgt ggtgagaact
    34141 acgacggcac caccggaacg tttgccgatc tgctgcgtct ggttgcggcg attgacggga
    34201 tcgatcgtat tcgctttacc accagccatc cgatcgaatt caccgacgat atcatcgaag
    34261 tgtatcgcga cacgccggag ctggtgagct tcctgcactt gccggtacag agcggttccg
    34321 atcgcattct gaacctgatg ggccgtactc acacggcgct ggagtacaaa gcgatcatcc
    34381 gtaaactgcg tgcggcgcgt ccggatattc agatcagttc tgacttcatc gttggcttcc
    34441 ctggcgaaac caccgacgac ttcgagaaaa cgatgaagct gattgccgac gtcaatttcg
    34501 acatgagcta cagctttatc ttctccgctc gtccaggaac gcctgccgcc gatatggttg
    34561 atgatgttcc ggaagaagag aagaagcagc gtctgtatat tctgcaagag cgcattaatc
    34621 agcaagcgat ggcatggagc cgccgtatgc tcggcaccac ccagcgtatt ctggtagaag
    34681 gtacatcgcg taagagcatc atggagcttt ccggtcgtac tgaaaataac cgcgtggtta
    34741 acttcgaagg cacgccggat atgatcggta aattcgtcga tgtagaaatt accgacgtct
    34801 atccaaactc cctgcgcgga aaagtggtgc gtactgaaga tgaaatgggt ctgcgcgtcg
    34861 cagaaacacc ggaatcggtg attgcccgta cccgcaaaga aaacgacctt ggcgtgggtt
    34921 attatcagcc gtaattctca ggcccgccgt tccggtgggc cttgtttttt ctctctttat
    34981 ccccatcttt atcgcaatgc ttgcgccttt ttcctgtggc gtgaataatt tccttatagg
    35041 ccggtgaatg ttcagcttcc ggcggcatac agataagagg aacggtttga acatagacac
    35101 tcgcgaaatc accctggagc cagcagacaa tgcgcgtctg ttgagcctgt gcggcccgtt
    35161 tgatgacaac atcaaacaac tcgaacgccg tctcggcatc gaaatcaatc gccgtgataa
    35221 ccactttaaa ctgaccggcc gtccgatttg cgtcaccgct gcggcagaca ttctgcgtag
    35281 cctgtatgtc gatactgccc cgatgcgcgg tcagattcag gatatcgaac cggaacagat
    35341 ccaccttgcg attaaagaag cgcgggtact ggagcaaagc gcggagagcg tgccggagta
    35401 cggcaaagca gtcaatatca aaaccaaacg cggcgtaatt aagccgcgca cgccaaacca
    35461 ggcgcagtac atcgccaata ttctcgacca tgacattacc ttcggcgttg gcccggcggg
    35521 tacgggtaaa acctacctgg cagtggctgc ggcagttgat gccctggagc gtcaggaaat
    35581 tcgccgtatt ctgctgactc gtccggcggt cgaagccggt gagaaactgg gcttcctgcc
    35641 tggcgattta agccagaaag tagacccgta tctgcgcccg ctgtacgacg cgctgtttga
    35701 aatgctgggc tttgagaaag tcgagaaact gattgagcgc aacgttattg aagtcgcacc
    35761 gctggcctat atgcgtggtc gtacgctgaa cgacgcgttt atcattcttg atgagagcca
    35821 gaacaccacc atcgaacaga tgaagatgtt cctgacccgt atcggtttta actcaaaagc
    35881 ggttatcacc ggcgacgtca cacagatcga cctgccgcgt aataccaagt ctggtttgcg
    35941 tcatgccatc gaagtgctgg ctgatgtcga agagatcagc tttaacttct tccacagcga
    36001 agacgtggtt cgtcacccgg tggtggcgcg tatcgttaac gcttatgaag cctgggaaga
    36061 agccgaacaa aaacgtaaag cggcgctggc ggcagaacgc aagcgcgaag aacaggaaca
    36121 aaaatgagtc aggtgatcct cgatttacaa ctggcatgtg aagataattc cgggttaccg
    36181 gaagagagcc agtttcagac atggctgaat gcggtgatcc cgcagtttca ggaagaatcg
    36241 gaagtgacta ttcgcgtggt cgataccgcc gaaagccaca gtctgaatct gacctatcgc
    36301 ggtaaggata agccgaccaa cgtgctctcc ttcccgtttg aagtgccgcc tggcatggaa
    36361 atgtcgctac tgggcgatct ggttatctgc cgtcaggtgg ttgagaagga agctcaggag
    36421 caaggcaaac cactggaggc gcactgggcg catatggtgg tgcacggcag tctgcatttg
    36481 ttaggttacg atcacatcga agatgacgaa gcagaagaaa tggaagccat cgaaacagag
    36541 attatgcttg ctctgggcta tgaggatccg tacattgccg agaaagaata atttgccagg
    36601 catttgactg gcagacgtcc ccgccgttgg tgattaacga cggcgttttg attaactaac
    36661 atgagaaccc atacgacgcc atgagcgacg acaattcaca cagtagtgac acgataagca
    36721 acaagaaggg atttttctcc ctgttactca gccaactttt ccacggtgaa ccgaaaaacc
    36781 gtgacgaact gctggcgctg atccgtgatt ccgggcagaa cgaccttatc gacgaagata
    36841 cgcgcgatat gctcgaaggg gtgatggaca tcgcagacca acgcgttcgc gacatcatga
    36901 tcccccgctc ccagatgatt accctgaaac gcaaccagac gctggacgaa tgccttgatg
    36961 tcatcatcga gtccgcccac tcacgtttcc cggtgattag cgaagacaaa gatcacattg
    37021 aagggattct gatggcgaaa gacttgctgc cgtttatgcg cagcgatgct gaagccttca
    37081 gcatggacaa agtgttacgt caggcggttg tcgttcctga aagtaagcgc gtagaccgga
    37141 tgctgaaaga gtttcgctct cagcgttacc acatggcgat cgtcattgac gaattcggtg
    37201 gggtttccgg tctcgtgacc attgaagaca tcctggaact gattgttggt gagattgaag
    37261 acgagtatga cgaagaagat gatatcgact tccgtcagct gagtcgtcat acctggaccg
    37321 tgcgcgcact ggcttctatt gaagacttca acgaagcgtt cggcacccac tttagcgatg
    37381 aagaggttga cactatcggt ggtctggtga tgcaggcatt tgggcatctt ccggcgcgtg
    37441 gcgaaaccat cgacatcgac ggttaccagt tcaaagtggc gatggccgac agtcggcgta
    37501 ttattcaggt tcatgtcaaa atcccggatg actcacccca gccgaagctg gatgaataaa
    37561 accgaaactg gatagataac tacatggctt ttgcctcatt aattgaacgc cagcgcattc
    37621 gcctgctgct ggcgttatta ttcggtgcct gcggaacgct ggccttctct ccttacgacg
    37681 tctggcctgc ggcgattatt tcgctgatgg gacttcaggc gctgaccttt aaccgccgtc
    37741 cactccagtc taccgctatt ggcttttgct ggggatttgg cctctttggc agcggtatta
    37801 actgggtcta tgtcagcatc gcgacctttg gcggaatgcc tggcccggtt aacatcttcc
    37861 tggtggtact gctggcggcg tatttgtcgc tgtataccgg actgtttgct ggcgtgctgt
    37921 cgcgtctgtg gccgaaaacc acctggctgc gcgtggcgat tgccgcccct gctctctggc
    37981 aagtgaccga gtttctgcgc ggttgggtac taacaggctt cccgtggtta cagttcggct
    38041 atagccagat tgatggcccg ttaaaagggc tggcaccgat aatgggcgtg gaagccatta
    38101 acttcctgct gatgatggtt agtggcctgc tggcactggc gttggtcaaa cgcaactggc
    38161 gtccgctggt ggtggccgtc gtgctgtttg cccttccctt cccgctgcgt tacgtccagt
    38221 ggtttacccc gcaaccggag aaaaccattc aggtttcgat ggttcagggc aatattccgc
    38281 aatcgctgaa atgggacgaa ggccagcttc ttaatacgct gaagatttac tacaacgcaa
    38341 cggcaccgct gatgggcaaa tcatcgctga ttatctggcc ggagtcggcg ataaccgatc
    38401 tggaaattaa tcagcaaccg ttcctcaaag ctctggacgg tgagttgcgc gataaaggta
    38461 gctcgctgat caccgggatt gtcgacgcgc gtctcaataa acagaaccgc tacgatacct
    38521 acaacaccat catcacactg ggtaaaggcg caccgtacag ctatgaatca gccgatcgct
    38581 ataacaaaaa ccatctggtg ccgtttggcg agtttgtccc actggagtcg attctgcgtc
    38641 cattagcacc gttctttgat ttgccgatgt cgtcattcag ccgcggacca tatattcagc
    38701 caccgctgtc gttgaatggc attcaactta ctgcggccat ttgctacgaa atcattctcg
    38761 gcgagcaagt gcgcgataac ttccgcccgg ataccgacta tctgctgact atctccaacg
    38821 atgcgtggtt tggtaagtct attggtccat ggcagcactt ccagatggcg cgaatgcgtg
    38881 cgctggagct ggcgcgccca ctgttgcgca gcaccaacaa cggcattacg gcggtgattg
    38941 gcccgcaggg tgagattcag gcgatgatcc cgcagttcac ccgcgaggtg ttaaccacta
    39001 acgtaacgcc gaccaccgga ctcaccccgt actcacgtac cggcaactgg ccactgtggg
    39061 tgctgacggc actgtttggc tttgccgctg tactgatgag tctgcgtgct cgcaaacgtt
    39121 aatgtgcctg atgcgcgacg cttatcaggc ctacgcgatt tttgcaatat gttgaatttt
    39181 cgcgattttg taggataaag cgtacttttt caacacgctc tggggcatga ttatcatgcc
    39241 cttttttctt acggttcagg gatacaagcc acatcgccat attggggatg ccgaataatt
    39301 gcctgcggat caagtgaact ttctctgtcc agttgtatta gcccgttctt cgccataaca
    39361 aatatctttc taccatccgc tgaaaaacga agatgattca cgtagctggc aggataaacg
    39421 ccaataatct gcccggcatt aatattgagc aggatgacat aaccttgcag gccatatgac
    39481 accaacgcta aatattgacc gtccgggcta ataccctcaa cggaataaac gctcctgcta
    39541 caccattgcg tcataatgct ggcttgttga tcctcagctc ttatacgtga aatcccctgg
    39601 ctatcggcaa aaataatctc accatttttt ggtcggaatc ccatcgcaga aacgacacca
    39661 cgcgcatcac ctgcgggaaa gtcttctttc tgatgcgtga tggtattgat ccgcacgatg
    39721 ttacctctgc ctgctcccgg aaccagtaat gttttttcat ccggggaaag tgccataacc
    39781 caggcgcctg gtttaccgct catccccgtc ccgtcgtatt tctccagcgg gatatccgcc
    39841 acatgctgat aagccaccag atcaatcgct gacacactat tactggtgcg attcatcacc
    39901 caggcttttg tgccatcacg cgagaggata aggctgtcag gatgtgcgcc ggtggtaatc
    39961 gactgaataa tctcccccgt tgccacgtta atgaccgata atgcattcgc ttcaaatgaa
    40021 gtcgcataca gcaacgtttc atccgggctt agcgccagcg acctgacgcc ctgatcttta
    40081 cagccaccac aatgtttgac tgacttttct ttcgctatgg gggctgtaaa ggtatgcagg
    40141 attttctttt gtccgaggtc gaaaacaaca atgctgttcg tgttttcaat tgcggcatac
    40201 aacgtattag catcccgact gatcgccata tcatggatat cggcaatctc agcgagtaaa
    40261 tcaccactgg cctgctcacg acttaccgga gcggataatg tttccctgag ggcgttgcgc
    40321 tcctccacta actgctgctt tttttgtcgt cgctcttgtt caatctcctg cggataaaac
    40381 gcttcacgca ggctttgcct gacctgtaaa actgattcat caatctgctg ctgcacaagc
    40441 cagatagtcc cggtcagaag aagagtgacc gcaacaataa tcccgccttc cgtaacgagc
    40501 cgattttgcc caactaaacg cgcactcagc caacccaacg cgccccccgc aatcaggccg
    40561 ccaatgtggc aagcgttatc tattcctgtt tgccgggtac catacaacag tgtcagcgcc
    40621 accataccca acagaggaaa tactcgccgc tggtttttat ggtgtgtgcc cgcacctgaa
    40681 gcaagctgtg ttgctatcgc agcgcccgct attcccataa ttgccccgga tgccccaacg
    40741 ctgatgacta tctgctcact ttcgcgtaac gcataacagg cgctgatcag cccaccaaag
    40801 atgccggaga ataaccagat aatcaggagt cgccatttcc ccagtatccg ttccgccact
    40861 gtccccacca ccaggagtgc aaacatattc atcagcaaat gggaaaaact actgtgcaga
    40921 aaaacactgc tcaataagcg ccaggattca tccgacaacg tgagagaagc aacgttagcg
    40981 ccgagatgga tgagtgcgga ttgatcgact tcttcgtaac tcatccattg tgcgaggtaa
    41041 caaaatatcc caacacagag cgcaaaaagg atcagggtta ttgcgggtaa ttgaacattt
    41101 aacggcttaa ccgaagatgc agacataagg tgattcttcc ttgaaaagac tagtaaaaag
    41161 cattttcccg tgtgtagtta tcatcccggc aaacgtccca caccgaaaag ttaacactgt
    41221 ccgaaatatg actttttcaa actgaaatta caatcgtaat taattcaccc ctaagtattt
    41281 tttggcatgt aagttgcttt atttactgtg taagcaatga aatcagccgc aacgcaaccg
    41341 ggttgcacca ccacaaatca gcggtagcac caaaaaggtg cggtttaaga attttgcttc
    41401 ggaatggtgc ggcgcacctc gcaaaaacaa acaataaaac agcaatatct ttacatttat
    41461 ccaaactaaa tgttacctaa caagcacaac actgcacaat aaagtcgcag atgataacaa
    41521 cacaaacact cacaacgggt atccatgcgt ttttaacgca gaagataaag gagttggata
    41581 tgcaattacg taaacctgcc acagcaatcc tggccctggc gctttccgca ggactggcac
    41641 aggcagatga cgccgccccg gcagctggca atacgctgga caaaatcgcc aaaaacggcg
    41701 tgattgtcgt cggtcaccgt gaatcttcag tacctttctc ttattacgac aatcagcaaa
    41761 aagtggtggg ctactcgcag gattactcca acgccattgt tgaagcagtg aaaaagaaac
    41821 tcaacaaacc agacttacag gtgaaattga ttccgatcac ctcacaaaac cgtattccac
    41881 tgctgcaaaa cggcactttc gattttgaat gcggttctac caccaacaat gccgaacgcc
    41941 aaaaacaggc ggctttctct aacactattt tcgtggtcgg tacgcgcctg ctgaccaaaa
    42001 agggcggtga tatcaaagat tttgccgacc tgaaaggcaa agccgtagtc gtcacttccg
    42061 gcaccacctc tgaagttttg ctcaacaaac tgaatgaaga gcaaaaaatg aatatgcgca
    42121 tcatcagcgc caaagatcac ggtgactctt tccgtaccct ggaaagcggt cgtgccgttg
    42181 cctttatgat ggatgatgct ctgctggccg gtgaacgtgc gaaagcgaag aaaccagaca
    42241 actgggaaat cgtcggcaag ccgcagtctc aggaagccta tggttgcatg ttgcgtaaag
    42301 acgatccgca gttcaaaaag ctgatggatg ataccatcgc tcaggtgcag acctccggtg
    42361 aagcggaaaa atggtttgat aagtggttca aaaatccaat tccgccgaaa aacctgaaca
    42421 tgaatttcga actgtcagac gaaatgaaag cactgttcaa agaaccgaat gacaaggcac
    42481 tgaactaatt acaagaacca ggggcggaaa ttccagccct ctcgattgtt acgtagcacg
    42541 gacagactat acgcctggtg gtcgttcccc atcgggcctg aaaaccgcaa tacgctgggt
    42601 aacaatcttc gagggtagca gttaacgctg ctaccctctt ttttctggag tagatttatg
    42661 tctatagact ggaactgggg tattttttta caacaagccc cgttcggcaa caccacctat
    42721 cttggttgga tctggagtgg ctttcaggtg acgatcgctt tgtcgatctg cgcctggatt
    42781 atcgctttcc tcgtcggttc atttttcggc attttacgta ccgttccaaa ccgttttctc
    42841 tccggtctgg gtacgttgta tgtcgaactg ttccgcaacg tgccgttgat tgtgcaattc
    42901 tttacctggt acctagtgat cccggagctg ctgccagaga aaatcggcat gtggtttaag
    42961 gcagagctgg atcccaatat tcagtttttc ctttcatcca tgctctgcct ggggctgttt
    43021 accgccgccc gtgtttgcga acaggtacgc gcagctattc agtcgctgcc gcgtgggcaa
    43081 aaaaatgccg cgctggcgat gggtctgacg ctaccgcagg cttaccgcta cgtactgctg
    43141 cctaatgctt atcgcgttat cgtcccgccg atgacctcag agatgatgaa cctggtgaaa
    43201 aactccgcca tcgcctcgac tatcggtctg gtggatatgg cggcgcaggc gggtaaattg
    43261 ctcgattact cggcccacgc ctgggagtcg tttaccgcca tcacgctggc gtatgtattg
    43321 attaacgctt ttatcatgct ggtgatgacg ctggttgaac gtaaagttcg cctgcccggc
    43381 aacatggggg gcaaataatg tacgagtttg actggagttc cattgttcct tccctgccat
    43441 atctgctcga cgggctggtg atcacgctga aaatcaccgt cacggcagtg gtgattggta
    43501 ttttgtgggg caccatgctg gcggtgatgc gcttgtccag ctttgcgccc gtagcgtggt
    43561 ttgccaaagc ctacgttaac gtattccgct cgattccttt agttatggtt ttgctgtggt
    43621 tttacctgat cgtaccgggt tttctgcaaa acgtgctggg attatcaccc aaaaatgata
    43681 tccgtttaat ttcggcgatg gtggcgtttt cgatgtttga agcggcctac tattcagaga
    43741 ttatccgtgc tggtattcaa agtatttctc gtggtcagtc gagcgccgcg ctggcgttgg
    43801 gaatgactca ctggcagtcg atgaaactga ttattctgcc gcaggcgttc cgcgcgatgg
    43861 tgccgctgtt gctcactcag ggcatcgtac tgtttcagga tacctcactg gtgtatgtgt
    43921 tatctctggc cgatttcttc cgtaccgcct caaccattgg tgagcgtgat ggtacgcagg
    43981 ttgagatgat cctgtttgcc ggatttgttt atttcgttat tagccttagc gcgtcgttgt
    44041 tggtcagcta cttgaaaaga aggacagcat aatgattacc ctgaaaaatg tttcaaaatg
    44101 gtatggtcac tttcaggtgc tgaccgactg ctcaaccgaa gtgaaaaaag gcgaagttgt
    44161 ggtggtttgc ggcccgtctg gttccggcaa atcaacgctg attaaaaccg tcaacggcct
    44221 cgaaccggtg cagcaaggtg aaatcaccgt cgatggcatc gtggttaacg acaagaaaac
    44281 cgatctggcg aaactgcgtt cccgcgtcgg gatggtgttt cagcatttcg agctgttccc
    44341 ccatctgtcg attatcgaaa acctgaccct ggcgcaggtg aaagtgctta aacgcaataa
    44401 agcgccggct cgtgaaaaag cattgaaatt gctggagcgc gtcgggcttt ccgcacatgc
    44461 caataagttt ccggcgcagc tttccggcgg ccagcagcag cgtgtggcga tcgctcgcgc
    44521 attgtgcatg gatcctattg ccatgctgtt cgacgaacca acgtcggcgc tggatccgga
    44581 gatgatcaac gaagtgctgg acgtgatggt cgaactggcg aacgaaggaa tgacgatgat
    44641 ggtggtgacc cacgaaatgg gctttgcccg taaagtggcg aatcgggtga tctttatgga
    44701 cgagggtaaa attatcgaag actcgccgaa agacgctttc ttcgataatc cgaaatcgga
    44761 ccgcgcaaaa gacttcctcg caaaaatcct gcattaatca ctctggcgcg catactcaat
    44821 tgcgcgccat ctcacgcttt ctcgtttcgt ccctcgtatc tgttacatcc cggcgttacc
    44881 cttgtcgcaa agaaacacac aacaaggagc aacaatggca ctgccaattc tgttagattg
    44941 cgacccaggt catgacgacg ctatcgcaat agttctcgcc ctcgcctcac cagagcttga
    45001 tgtcaaagca attacgtctt ccgccggaaa ccagacacca gaaaaaacct tacgcaatgt
    45061 tctgcgtatg ctgaccttgc ttaatcgcac cgatattccg gtagcaagcg gtgcggtaaa
    45121 accgttaatg cgtaatttga ttattgcgga caacgtgcac ggcgaaagcg gcctggacgg
    45181 cccggcattg ccggaaccgg catttgcacc gcaaaactgc acggcagtgg agctgatggc
    45241 gaaaacgctg cgtgaaagtg cggaacctgt caccattgtt tctaccggac cgcaaactaa
    45301 cgttgccttg ctgctcaata gccacccgga actgcatagc aaaattgccc gtatcgtgat
    45361 tatgggcggc gcaatggggc ttggtaactg gacgcctgcg gctgaattta atatttacgt
    45421 tgacccggaa gcggcagaaa ttgtcttcca gtcaggaatt ccggtggtga tggccggtct
    45481 ggatgttact cacaaagcac aaatccacgt tgaagacact gagcgtttcc gcgcgattgg
    45541 caaccctgtt tcaaccattg ttgccgaact gctggatttc ttcctcgaat atcacaaaga
    45601 cgaaaaatgg ggctttgtcg gcgcaccact gcatgaccca tgcaccatcg cctggctgtt
    45661 gaaaccggag ttgtttacca ctgttgagcg ctgggttggc gtggaaacac aagggaaata
    45721 tacccagggt atgacagttg ttgattatta ttatctgact ggcaataaac cgaatgccac
    45781 cgtaatggtc gatgttgatc gtcagggctt tgttgattta ctggctgatc gtctgaaatt
    45841 ttacgcttaa catacgtcat tgattaagat caccacgcat tacctgggtg gtcttaatct
    45901 ggttatctct ttttctttca agatataaat gcaatggata atgcagaact tgctattggt
    45961 atcgatctcg gtactaccaa tagtttaatt gccgtctgga aagacggtgc cgcgcaatta
    46021 attccaaata agttcggtga atatttaaca ccatccataa ttagcatgga tgaaaataat
    46081 catattttag tcggaaaacc ggctgtatca cggcggactt cgcatccgga taaaacggca
    46141 gcgttattta aacgtgcaat gggcagtaat accaactggc ggttaggcag cgacacattt
    46201 aacgcgccag aactgtcctc tttggtatta cgctcattaa aagaagatgc cgaagaattt
    46261 ctgcaacgtc cgattaaaga tgtggtgatc tccgttcctg cttatttcag cgatgaacaa
    46321 cgcaagcata cccgtttagc agcagagtta gccgggctaa atgcagtgcg cctgattaat
    46381 gagccaacgg cagccgcgat ggcgtatggc ctgcatacgc agcaaaatac ccgctcgctg
    46441 gtttttgatc tcggtggcgg cacgttcgac gttacggtgc tcgagtacgc cacgccggtg
    46501 attgaagtta acgcctccgc tggcgataac ttcctcggtg gcgaagattt tacccatatg
    46561 ctggtcgatg aggttttaaa acgcgcggaa gttgccaaaa ccacgcttaa cgatagcgaa
    46621 ctggcagcct tgtacgcctg cgtggaagcg gcaaaatgta gcaatcaatc gccaatgcac
    46681 attcgctggc agtaccagga tgaaatgcgg aaatgcgaat tttacgagaa cgaactggaa
    46741 gatttgtggt tgccgctgct caatcgcttg cgagtgccga ttgaacaggc gttgcgcgat
    46801 gcacgtctga agccgagtca aatcgacagt ctggtgctgg ttggcggcgc gtcacaaatg
    46861 ccgctggtgc agcgaatcgc agtgcgtctg tttggcaaat taccgtatca aagttacgat
    46921 ccgagcacca ttgtcgcgct gggcgcagca atccaggccg cctgccgctt acgcaatgaa
    46981 gatattgaag aagtgatcct cactgatatt tgcccttact cgttgggtgt tgaagttaac
    47041 cgtcagggcg tatccggcat tttctcgccg attattgaac gaaacaccac agtaccggtg
    47101 tcgcgtgtag aaacttattc aaccatgcac ccggaacagg attcaattac ggttaacgtc
    47161 tatcagggag aaaaccacaa agttaaaaat aacattcttg tggaatcctt cgatgtgccg
    47221 ttgaagaaaa ccgggactta tcagtcgatt gatattcgct ttagttatga tatcaacggc
    47281 ttgcttgaag ttgacgtgct tctggaagac ggcagcgtta agtccagagt gattaatcac
    47341 agtccggtaa cattgagcgc gcagcagatt gaagagagtc ggacgcggtt atccgcattg
    47401 aaaatttatc cgcgcgatat gctcatcaat cgcaccttta aagccaaact ggaagagttg
    47461 tgggcgcggg cgctgggtga cgagcgagaa gagattggtc gggtgatcac cgattttgat
    47521 gcggcgttgc agtcaaacga tatggcccgc gtggatgaag ttcggcggcg ggcgagcgat
    47581 tatttagcca ttgagatccc ataaacacgg ctaccggatg cggcgtaaac gctgcatccg
    47641 gcatcggcgc aaaaaacaca tcgtcaacta aacaacatcg cgattttgcc gagagcataa
    47701 cctatcacca gcaccgttaa actgattttg ataatgctgg taatgctctg tggtttttca
    47761 tcctcatcat ttatttcttc atggttttca ggtgttggct catcgacatc ttcatcctcc
    47821 ggctcgagct tcactttcgt caacagcatt cgggtgataa tggtctctaa tggatgtgcc
    47881 tctccgcgtt ccagccgcgc gtccagacgg gccagcatct cttgtttcgg aaacgcttca
    47941 agtactacct gttccgctac ccgatacaaa tccagtaacg gtgaatcttg ctctgtctcg
    48001 ctaccgactc tcgatttcca ccacgcccat gctttgcgtt tgaagaataa cgactcaata
    48061 aacgattgac ctaacggcga taattttccg ctatgtaaaa tctccgccca taacagccgc
    48121 gtccgggcgc tctcaccagg aacatatttc aacggcttac cttgcgcatc taccatgctc
    48181 cgcgcttcga taaccatcac taataacgcg aaataatccg ggcaatgacg cttgctccac
    48241 tgcaacaaca aattatcggc ttgtgtagat gggtaacttt cccggtacgc gcagagatcg
    48301 gcaagcaggc tttccccctc gccgcagtac aaccgttgtg cgcactggta ataacgggca
    48361 tcttcgcttt ctggttcttc cgcttgccac tgttgagcga caggaatgag ttccgccata
    48421 cctgtttgca ccgagctgta ccagcgcaac agtttgcgat gcaacttcgc atcatcagga
    48481 atgaaccacg gaccttccat cgccaaaaac gcagtgacat agtcaggatg gtaacggcag
    48541 caacgttcca gcgagaagta atactcaacc gtctggttct gcaacgcaac gggatattcc
    48601 agaaggctaa ggaaatcgaa acaatcacca gcctgaatgg cctcaagaaa agcctccctt
    48661 tcttcctcat ctattccttc gcagtcatta aacgaatgcc agttcaaacg ctctgccagc
    48721 aaagaagcac aatttaatga aagatatcgc gcttctatag cgattgcaca cagcggccag
    48781 cgtaattgat cgacatcctc catgttccag gtatttaatt gctgaataaa tttctgccag
    48841 gcggagggct gaaaacgatc attttctgaa tccagtaatg ccctgaatac acgtagtatt
    48901 tcatgctcag tggcagcatc tttttcttca tcatctgctt cctcgacagg atttaccgcc
    48961 agtcgcagcg cctcttcata ggcctggcgt aatgctttaa acccttgtgg atcggtttcg
    49021 ggatgacata acggtaagcg agcaagataa gcctggcgga taatgtctat ttgcgtcgtg
    49081 ctttcaattt cgaggatttg ccaacatgtt ttcataggtc gctcacatcc ttaagcgtta
    49141 ccgggcattc aggctggaca gttaatggct gccagggaag cgtggcatag aaacggtcgc
    49201 ttcccgctgc cagctcttcg aacatccgac tcccggcgct atcgtatttg tacagggtta
    49261 gcgcctcgcg tagcgaagcg ttgtcttcgc actgagaaga ttgccagtag agacgcccta
    49321 acgaataggc agaaaaatag tgcatccagc tgtgatagta atggtgtgct cgtatataga
    49381 cgcgcgattg cagccataaa ctctcgtctt ccgtcaacca cttgttgagc acaccaaccc
    49441 gtgataaaaa acccatccgc acataatccc aggctttgat tcccccttcc ccacaacaca
    49501 tagcggtttg ggcaacgaat tgggtataaa tcaaccctct ctccgagccg ccagcaatta
    49561 atgctttcca ctcttcaggg gaagagcgat gccatgtcag atataagcca gctaaatcat
    49621 tggcatgacc atcatcggtc atctgataaa tagtctgaat taaatcatca cgattattta
    49681 ttccccagga tgatgagaga tcgaattctt ttatcccggt atacattttt ggcgagtcat
    49741 gcgcgggctt gcaaccctga ttaagtattt cc
//
LOCUS       DACADF010000013        49705 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.013, whole genome shotgun sequence.
ACCESSION   DACADF010000013 DACADF010000000
VERSION     DACADF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.013"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            77..709
                     /gene="comR"
                     /locus_tag="ISB30_000757"
     CDS             77..709
                     /gene="comR"
                     /locus_tag="ISB30_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAP1988427.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDHYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(771..1310)
                     /locus_tag="ISB30_000758"
     CDS             complement(771..1310)
                     /locus_tag="ISB30_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAP1988428.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1537..2841)
                     /locus_tag="ISB30_000759"
     CDS             complement(1537..2841)
                     /locus_tag="ISB30_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP1988429.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3240..3782)
                     /locus_tag="ISB30_000760"
     CDS             complement(3240..3782)
                     /locus_tag="ISB30_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1988430.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3805..4830)
                     /gene="nagZ"
                     /locus_tag="ISB30_000761"
     CDS             complement(3805..4830)
                     /gene="nagZ"
                     /locus_tag="ISB30_000761"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP1988431.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4841..5665)
                     /gene="thiK"
                     /locus_tag="ISB30_000762"
     CDS             complement(4841..5665)
                     /gene="thiK"
                     /locus_tag="ISB30_000762"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAP1988432.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRL
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5646..6287)
                     /gene="lpoB"
                     /locus_tag="ISB30_000763"
     CDS             complement(5646..6287)
                     /gene="lpoB"
                     /locus_tag="ISB30_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAP1988433.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPTEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6301..6678)
                     /locus_tag="ISB30_000764"
     CDS             complement(6301..6678)
                     /locus_tag="ISB30_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAP1988434.1"
                     /translation="MRKGCFGLVSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6681..7040)
                     /gene="hinT"
                     /locus_tag="ISB30_000765"
     CDS             complement(6681..7040)
                     /gene="hinT"
                     /locus_tag="ISB30_000765"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAP1988435.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7374..9563
                     /gene="fhuE"
                     /locus_tag="ISB30_000766"
     CDS             7374..9563
                     /gene="fhuE"
                     /locus_tag="ISB30_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HAP1988436.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDRSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTKWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNLSITGTYQF"
     gene            complement(9623..11056)
                     /gene="ptsG"
                     /locus_tag="ISB30_000767"
     CDS             complement(9623..11056)
                     /gene="ptsG"
                     /locus_tag="ISB30_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAP1988437.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLT
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11351..12148)
                     /locus_tag="ISB30_000768"
     CDS             complement(11351..12148)
                     /locus_tag="ISB30_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP1988438.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12159..13163)
                     /gene="holB"
                     /locus_tag="ISB30_000769"
     CDS             complement(12159..13163)
                     /gene="holB"
                     /locus_tag="ISB30_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP1988439.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYGVQSGDWYSLLAALNNEQAPARLHWLATLLMDALKRHYGAAHVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13160..13801)
                     /locus_tag="ISB30_000770"
     CDS             complement(13160..13801)
                     /locus_tag="ISB30_000770"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP1988440.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(13791..14813)
                     /gene="yceG"
                     /locus_tag="ISB30_000771"
     CDS             complement(13791..14813)
                     /gene="yceG"
                     /locus_tag="ISB30_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAP1988441.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14816..15625)
                     /gene="pabC"
                     /locus_tag="ISB30_000772"
     CDS             complement(14816..15625)
                     /gene="pabC"
                     /locus_tag="ISB30_000772"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAP1988442.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15745..16986)
                     /gene="fabF"
                     /locus_tag="ISB30_000773"
     CDS             complement(15745..16986)
                     /gene="fabF"
                     /locus_tag="ISB30_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP1988443.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17074..17310)
                     /gene="acpP"
                     /locus_tag="ISB30_000774"
     CDS             complement(17074..17310)
                     /gene="acpP"
                     /locus_tag="ISB30_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP1988444.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17521..18255)
                     /gene="fabG"
                     /locus_tag="ISB30_000775"
     CDS             complement(17521..18255)
                     /gene="fabG"
                     /locus_tag="ISB30_000775"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAP1988445.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18268..19197)
                     /gene="fabD"
                     /locus_tag="ISB30_000776"
     CDS             complement(18268..19197)
                     /gene="fabD"
                     /locus_tag="ISB30_000776"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP1988446.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19213..20166)
                     /locus_tag="ISB30_000777"
     CDS             complement(19213..20166)
                     /locus_tag="ISB30_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP1988447.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLTANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20234..21304)
                     /gene="plsX"
                     /locus_tag="ISB30_000778"
     CDS             complement(20234..21304)
                     /gene="plsX"
                     /locus_tag="ISB30_000778"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP1988448.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21385..21558)
                     /gene="rpmF"
                     /locus_tag="ISB30_000779"
     CDS             complement(21385..21558)
                     /gene="rpmF"
                     /locus_tag="ISB30_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP1988449.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21610..22131)
                     /gene="yceD"
                     /locus_tag="ISB30_000780"
     CDS             complement(21610..22131)
                     /gene="yceD"
                     /locus_tag="ISB30_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAP1988450.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22330..22914
                     /gene="yceF"
                     /locus_tag="ISB30_000781"
     CDS             22330..22914
                     /gene="yceF"
                     /locus_tag="ISB30_000781"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAP1988451.1"
                     /translation="MPELILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23026..23985)
                     /gene="rluC"
                     /locus_tag="ISB30_000782"
     CDS             complement(23026..23985)
                     /gene="rluC"
                     /locus_tag="ISB30_000782"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAP1988452.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAR"
     gene            24558..27743
                     /gene="rne"
                     /locus_tag="ISB30_000783"
     CDS             24558..27743
                     /gene="rne"
                     /locus_tag="ISB30_000783"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAP1988453.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     PRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQTPRRERSRR
                     RNDDKRQAQQEVKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAAAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAAEPVVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVTAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27938..28891)
                     /gene="flgL"
                     /locus_tag="ISB30_000784"
     CDS             complement(27938..28891)
                     /gene="flgL"
                     /locus_tag="ISB30_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAP1988454.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28903..30546)
                     /gene="flgK"
                     /locus_tag="ISB30_000785"
     CDS             complement(28903..30546)
                     /gene="flgK"
                     /locus_tag="ISB30_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HAP1988455.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDTSAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30612..31553)
                     /gene="flgJ"
                     /locus_tag="ISB30_000786"
     CDS             complement(30612..31553)
                     /gene="flgJ"
                     /locus_tag="ISB30_000786"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAP1988456.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPIKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31553..32650)
                     /locus_tag="ISB30_000787"
     CDS             complement(31553..32650)
                     /locus_tag="ISB30_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAP1988457.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32662..33360)
                     /gene="flgH"
                     /locus_tag="ISB30_000788"
     CDS             complement(32662..33360)
                     /gene="flgH"
                     /locus_tag="ISB30_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAP1988458.1"
                     /translation="MQKNAAHTYAISSLLALSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33414..34196)
                     /gene="flgG"
                     /locus_tag="ISB30_000789"
     CDS             complement(33414..34196)
                     /gene="flgG"
                     /locus_tag="ISB30_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAP1988459.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34368..35123)
                     /locus_tag="ISB30_000790"
     CDS             complement(34368..35123)
                     /locus_tag="ISB30_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HAP1988460.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLVVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35143..37068)
                     /gene="flgD"
                     /locus_tag="ISB30_000791"
                     /pseudo
     CDS             complement(35143..37068)
                     /gene="flgD"
                     /locus_tag="ISB30_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(37080..37484)
                     /gene="flgC"
                     /locus_tag="ISB30_000792"
     CDS             complement(37080..37484)
                     /gene="flgC"
                     /locus_tag="ISB30_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAP1988461.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIQSQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37488..37904)
                     /gene="flgB"
                     /locus_tag="ISB30_000793"
     CDS             complement(37488..37904)
                     /gene="flgB"
                     /locus_tag="ISB30_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAP1988462.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPSAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38059..38718
                     /gene="flgA"
                     /locus_tag="ISB30_000794"
     CDS             38059..38718
                     /gene="flgA"
                     /locus_tag="ISB30_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAP1988463.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMISGQVVSGVVDADGNILIN
                     L"
     gene            38794..39087
                     /gene="flgM"
                     /locus_tag="ISB30_000795"
     CDS             38794..39087
                     /gene="flgM"
                     /locus_tag="ISB30_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAP1988464.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39092..39508
                     /gene="flgN"
                     /locus_tag="ISB30_000796"
     CDS             39092..39508
                     /gene="flgN"
                     /locus_tag="ISB30_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAP1988465.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPYRGSKKISI"
     gene            complement(39548..41083)
                     /gene="murJ"
                     /locus_tag="ISB30_000797"
     CDS             complement(39548..41083)
                     /gene="murJ"
                     /locus_tag="ISB30_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAP1988466.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41193..42116)
                     /locus_tag="ISB30_000798"
     CDS             complement(41193..42116)
                     /locus_tag="ISB30_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP1988467.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFGVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRTNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGNATLESGTLLTNESGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVDCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42118..42765)
                     /locus_tag="ISB30_000799"
     CDS             complement(42118..42765)
                     /locus_tag="ISB30_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAP1988468.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42776..43360)
                     /gene="rimJ"
                     /locus_tag="ISB30_000800"
     CDS             complement(42776..43360)
                     /gene="rimJ"
                     /locus_tag="ISB30_000800"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAP1988469.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALSAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43596..44804
                     /gene="mdtH"
                     /locus_tag="ISB30_000801"
     CDS             43596..44804
                     /gene="mdtH"
                     /locus_tag="ISB30_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAP1988470.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44868..45515
                     /gene="grxB"
                     /locus_tag="ISB30_000802"
     CDS             44868..45515
                     /gene="grxB"
                     /locus_tag="ISB30_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAP1988471.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45648..46208
                     /locus_tag="ISB30_000803"
     CDS             45648..46208
                     /locus_tag="ISB30_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP1988472.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46314..47357
                     /gene="pyrC"
                     /locus_tag="ISB30_000804"
     CDS             46314..47357
                     /gene="pyrC"
                     /locus_tag="ISB30_000804"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP1988473.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVK"
     gene            47434..47679
                     /gene="dinI"
                     /locus_tag="ISB30_000805"
     CDS             47434..47679
                     /gene="dinI"
                     /locus_tag="ISB30_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAP1988474.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47968..48222
                     /gene="bssS"
                     /locus_tag="ISB30_000806"
     CDS             47968..48222
                     /gene="bssS"
                     /locus_tag="ISB30_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAP1988475.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            48337..49455
                     /gene="solA"
                     /locus_tag="ISB30_000807"
     CDS             48337..49455
                     /gene="solA"
                     /locus_tag="ISB30_000807"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAP1988476.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLHAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSADQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            49503..49616
                     /locus_tag="ISB30_000808"
     CDS             49503..49616
                     /locus_tag="ISB30_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAP1988477.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        12062 a        13364 c        12320 g        11959 t
ORIGIN      
        1 ggtttctgga ctggtgaacg gaggtgatta tttttataat aacctttcat tcacagtcac
       61 caggtacaac ggcatcatgg caactgactc aacacaatgt gtaaaaaaaa gccgtggccg
      121 cccaaaagtg ttcgacaggg atgccgcgct tgataaggcc atgaaattgt tctggcagca
      181 cggttatgaa gcgacttctc ttgcggacct cgttgaagcg accggagcta aagcgcccac
      241 gctatacgcg gaatttacca acaaagaggg gttattccgc gccgtactcg accactatat
      301 cgatcgtttt gccgccaagc atgaagcaca gctgttttgt gaagagaaaa gcgtggagtc
      361 tgcgctggct gactattttg ctgccatcgc caactgcttt accagcaaag acactccggc
      421 tggctgcttc atgatcaaca actgcaccac cctctccccg gattcaggag atatcgccaa
      481 tacgctgaaa tcacgccatg cgatgcaaga gcgcactttg cagcagtttt tatgtcaacg
      541 acaagcgcgc ggggaaatcc cgacccactg tgacgtgacg catctggcag aattccttaa
      601 ttgtattatt caggggatgt cgatcagcgc acgcgaaggt gcatcactgg aaaaactgat
      661 gcagattgcc cgaacgactt tgcgtttatg gccggaactg ctgaaatagg tcgcaaaaaa
      721 agcccctcag cctgagcgaa tgaggggcta aattgcagag tacagccttg ttatactttg
      781 ttattcaaaa tcagctgccc gttgctatct agcgggatct gcgtacccgg atcgcggtcc
      841 atgcggattt tgccctgctg atcgccaatc ttataggtca catcataacc gagcattttt
      901 tctgacttgt catacaccgt tttacaacgc tgttgcgtag tcgtgtaagt atcgctttcc
      961 tggagagagc cctggatctg gttacctgca tatccacccc ccagcgcccc cacaacagtg
     1021 gcgacatctt taccgcgacc acctccaaac tgatgcccaa tcacgccgcc agcaacagcg
     1081 ccgagcaccg acccggtaat gcgattttca tcctgcaccg gtcgacgatg ggtcactgta
     1141 acgttgcgac actcctgacg cggtgtttta accgtttcct tgattggggt tgcagaaaca
     1201 acctgagcgt attgcgggcc acgttcaaac acgttcagac tggccactgc cgctacgccc
     1261 agcgcagctg cgacaccaat cccgataccc gccaacattg acttattcac gggaacacct
     1321 ccttctttgt agttatgcca atcatgcaag cgaaggagaa aagcagctat cagacagaag
     1381 atgaaaagcg cgggaatggc tggaaatact cagggattcg gaggagtctg aaggaatgcc
     1441 tgatgcgacg cttaacgcgt cttatcaggc ctaccgctcg taccatttgt ccagggcgga
     1501 taaggcgttt atgccccatc cgccagtaca agtcgattaa tgcaacttca aacgtggacg
     1561 gataacgcgg ttaatactcc ccaccagcat cattaatccg gttttaaagt aaccatgcag
     1621 cgcaatctgg tgcattcggt acagcgagat gtatacaaag cgcgcaattc gtccttcaat
     1681 catcattgag ccgcgcgtca ggttacccat caggctaccg actgtggaaa agttcgacag
     1741 cgataccagc gaaccgtgat ctttatactg ataatttttc agcggcttac cgttcatctg
     1801 cgccagaatg ttgttcattg cgcaggttgc catctgatgt gcggcctgag cgcgcggcgg
     1861 aacaaagccc ccttccggac gcgggcatga cgcgcagtcg ccaatagcgt atatgtctgg
     1921 atcgcgagtg gtttgcagcg tcggttccac caccagctgg ttgatacggt tggtttcaag
     1981 accaccgata tctttcagga agtctggcgc tttgatcccg gctgcccaca ccatcagatc
     2041 agcctcaata tattcgccat ctttagtgtg caggccgcct tcatcagcac tggtgaccat
     2101 ggtttgcgtc agcacgcgaa cgccaagttt cgttaactca ctatgggccg cagcagagat
     2161 acgtggcggc aatgcaggca aaatacgttc gcccgcttct accagcgtta cgttcagggc
     2221 ttcgttggtc agacctttat aaccgtaact gtgcagttgc ttgaccgcgt tgtgcaattc
     2281 agcggagagt tctacacccg tcgcgccgcc gccgacaatc gcaatgttta ctttgccatt
     2341 cgcgcccagg ttggcggagt atttcaggaa cagattcagc atctcctggt ggaagcgacg
     2401 cgcctggtgc gggttatcga ggaaaatgca gttctctttg acacctggtg tattgaaatc
     2461 gttagaggtg ctacccagcg ccattaccag ggtgtcatag gcgattttac gttccggaac
     2521 cagcagttca cctttctcgt cgcgcagttc tgcaatagtg attgttttcg cttcacgatc
     2581 aatatcaatt acggaaccca gctggaactg gaaaccatga ttgcgcgcat gggccagata
     2641 gctcaacgca tcgacgcctt catcaagcga gccagtcgcc acttcgtgca gcagcggttt
     2701 ccacaagtgg ctgtggttac gatcgaccag cgtaattttg gcttttttct tgcgtcccag
     2761 cttatgcccc agctgtgttg ccatttccag cccaccagca ccgccgccga caatcacaat
     2821 ttttttcaat ggcgtagtca acgtgacccc cttaaattta ttaaccaatt gttaattaat
     2881 ggttattaac atagctctta attaacaaca ggttatagca atgaaaatgt tcttcgctat
     2941 tgagaataac atgaatggtg cattggtcat accaaaattg atgttaatca agttttgttg
     3001 ctgaaaagat aggcgacttt accgggtgag atcaaaaaac agagtttgtt gttcattatt
     3061 tgagctgatt tgtgagaatg atatagagtg aggtgtgtat ggagggcgtt cggtaggcct
     3121 gataagaggc gcatcaggca ttgatgctcg actgtcggat gcggcggaac gccttatcca
     3181 acccacagaa tcaggagcgc atcaggtacc ggcaaggtta cctgatgcga cgagggcatt
     3241 tacccgaggg ttttgaacgc tttaatgcgc tgtaaatgcg gggagatatt cttgaatttg
     3301 tgcgtctgct cttcgtccca gacaatctcg taataatgat gcaactcttc agatgtccgc
     3361 tggctgttaa gcgcttcatc attacgcgac aaaatcacca ggcaacgatc gcgattcttc
     3421 tcacggaagt tggtcacaca cttagtggca atatcggcat actcttccgg gcgatcaatc
     3481 ttcccttcca tgttctcata agggaacaaa ttggggttga agatcacctg gcggatatca
     3541 cagagaaaac caatccgctc agcccaataa ccgcccagac caacaccaca aatgagcggg
     3601 cgctcgtcga cgttcagttg caacattttg tccacttctt tgagcaaatg ctgcatatca
     3661 tgttttggat gccgcgtact gtagcttatt agccgtacat ccgggtcaat aaactgcaat
     3721 tgtaagactt tctcgtggtt acccggactg ttagagtcaa aaccgtgcaa atagataatc
     3781 atcgcttcct cacataagcc agggttaatg acctgccttc tcttcctgcc agcgttcatg
     3841 taactgattc agacgggcgc tgatcgcttt ccagcgagcc gagtccatca gttcctgtcg
     3901 cgaaaatgaa cctttatgat acaaacgtgt aacacgttct gccttgatcg gtgacagatt
     3961 atctaacacg ctgacggccc ctttacgatt attgcagacc aggatcatat cgcaacctgc
     4021 atccagtgat gcctgtccac gctcggcata actgcccata atcgctgcgc cttccatcga
     4081 taaatcgtca gagaaaatca cgccgtcaaa acccaattcc tgacgcaaaa cggttttcag
     4141 ccagtaggga gaaccgctcg ccggacgcgg atcaacatca ctgtagatca catgcgcagg
     4201 cataatggcg tcgagtttat tttcgcggat taaggaactg aagaccgaca tatctttagc
     4261 gcgaatctcc gcttgcggac gtgggtcgca cggtgtttct ttgtgtgagt ctgccgttac
     4321 tgcaccgtga cctgggaagt gtttcccggt agttttcatt ccggcttcat gcataccatc
     4381 aataaaccgg ctggcaattg ccagggcttt ttgtggatcg gcatgataag aacgctcgcc
     4441 aatcgccgcg ctgatatgcc cgacgtccag cactggcgca aagctgatat caatatccat
     4501 agcaatcatt tcgctggcca tcaaccaacc tgcctcttgc gccagttttc cgccttcttc
     4561 cattcccaac agcgcagcga atgattgtgc cgctggcaag cgggtaaaac cttcacgaaa
     4621 acgctgcacg cgtccacctt cctgatctac cgccaccacc agatgatttc gcgatgctgc
     4681 gcggatctgg cgcaccagtt cacgtaactg ggcaggatca tgatagtttc gcgtaaagag
     4741 aatcagccct cccaccagcg gatgcgccag tatttcacgc tcttccgcgt ccagttcgta
     4801 accttcgaca tccaacatta ctggacccac actgctctcc ttattgtttt attaatagct
     4861 gccgccaggt gtcatcggcc agcctgataa attgttgatc gccggtttgt cgccagcggt
     4921 actcaaacca ccctgctttg agcatcagca gccagggaaa ccatcgcctg acctgacgcc
     4981 ataattgcgc cggataaatc ttcgcgcgag tggcatagtc attgaccaat tgccgatgct
     5041 ggtcagtatt ttccacccac accgccgcca gttccagcgc gatatcacca tctccggcat
     5101 actcccagtc gatgagtttt aaccctgacg cgctatgcac taaatttccg gcgtggacat
     5161 ccatatgcaa tggacttaag cgtaaaagcc gtggttcccg cgctttgcgc agacgtttta
     5221 acattcgcag ccaacccact gtccgccgcg ccggatcgct ttgctgccag tacagttcca
     5281 gtaacggcaa cagcgttatt cgccagccaa aacgcggttg ttgatgtaga taatacagca
     5341 agcctgccag ttcgttggta tccggcaaat acgtttttac ctcgccgggc agatagtcaa
     5401 ccaccatcca gtcacggaga tataaatgcg gcttcggtgc aatgcatgcg ggtagttgtg
     5461 ataaagcccg atactggcgc aagaacgcgg actgcggcgc atcaggatcg tgcggctgac
     5521 gcacaacaaa acgctggccc tgatgttcaa tgagaaaact cccgccacta agcccactat
     5581 taaacgtcgt gacgggatga aactgcggga aaaagcgcga cagcaattcg tcgcgcgtga
     5641 ggggattatt gctgcgaaac ggcaccttta cctgaccaga taatttcgcc cgtctgcacc
     5701 agcatcagct gcatttgtag ggtcggagcg ttaacgttgc cagaggcgct ggagtacagc
     5761 acgtaatgag cgccaacatt gcgggcaatg cctatggctt tactacgggt gcctaaactg
     5821 tcctgcggcg acaaacctaa ttgttgctta gccatcgaca gctgctgggc ggaaaccagg
     5881 gtaaatttac cgttattagc cagcgcattt cgcagcgttt cggtcgcttc tgcggcattc
     5941 aacgaaccgt tagtacggtt gttaacgcta tcaaccagca ggacgctacc cgcagtcacg
     6001 ccatcagccc caagcatctt actgaccatc ggctgcattg cgccattcca gtcataatgg
     6061 cgaatatgcg gcgcaggcgg tgcagtttga tcttcgtgct caattgggcc tggctgctgc
     6121 ggtatcgtcg gcaccgatgg cactgtgggg acaggctgtt gtggctcggt tggttgttcc
     6181 ggcgctggtt tcacttcttc taccggtgca ggttcacgtt gccccacaca cccggcaaga
     6241 aacatcgcca gcgcggtaat caaggcgtag cgactcattt ttgtcatcaa gattcacccc
     6301 ttacaaatat agataaagtc tgactttgtg cgcccccaga aaattggcgc tgccgtacag
     6361 cgtgaccgcc gaatgtgcgg gaatggtaac gctgcgtggc ctttccagag gatgcatctc
     6421 cagccctctg gcgtcatacc agtaaaaacg ataatgaacg gtaacgggtt cttgcctttc
     6481 gttataaagc gttgaggatg ctgaaggttg aatatcagat gtcgaaagga cgggcttttc
     6541 tgcactgatg cccgcagcca ataaagatga ctccatcacc agcgattgct catcattcac
     6601 cggaatttcc ggatgtgaac gacagcccac cagcactaac aacgccagag acaccagccc
     6661 aaagcatcct tttctcatcg ttacagacct ttatgcgcca gcattggtcc cagcggacgg
     6721 ccacccaaca agtgcatatg gatgtggtaa acctcttgtc cgccatggcg gttggtgttc
     6781 atgatcagac gatagccatc ttcggcaata ccttcttgct cagcaatttt tgccgctacg
     6841 gtgatcatgc gtcccagcgc ctgctcatgc tcagctgaca cgtcgttcac agtcggaatg
     6901 aggatattcg gaatgatcag aatatgcgtt ggcgcttgcg gcgaaatatc gcgaaacgcc
     6961 gttaccagat catcctggta gacgatatcg gaggggatct cacgacgaat aattttgctg
     7021 aatatagttt cttctgccac gacattttcc tttttcaaat agccatgcgt tgcgccatgc
     7081 tacgccgcgc cacactgcga gtatagagta tggtcgagtt aaacctgctc tttcaacctt
     7141 aactcacgat ttcttaagca aaaaaccgtt ttgctgccgt atatatcgcc attattccca
     7201 tttctgcctg cgaatgcgct gaaaagacat tttctgtttg gtttacacac caaatatgaa
     7261 tgcgtatatt tctcatttgc atttacatac aaaattattc gcacactaaa tataaccgct
     7321 catcaccctg ctccggcatg agcctgacgc caacataaac caagagattt caaatgcttt
     7381 cgacacaatt taacagggat aatcaacatc aagccatcat caaaccgtca ctccttgccg
     7441 gttgcatagc actggcacta ttaccttccg ccgcttttgc tgcaccagcc actgaagaaa
     7501 cggtgattgt tgagggttca gccacagccc cagatgatgg cgaaaatgat tacaacgtaa
     7561 cgtctacctc tgcgggtaca aaaatgcaga tgactcaacg tgatattcct cagtcggtca
     7621 ctattgttag ccagcagcgg atggaagatc agcagttaca aacgctgggc gaagtgatgg
     7681 aaaacacgct ggggatcagc aaaagtcagg cggattccga tcgtgctctt tattattccc
     7741 gcggattcca gatcgataac tatatggttg atggtatccc cacctatttt gaatcgcgct
     7801 ggaatctggg cgacgcactt tctgatatgg cactgtttga acgcgtagaa gtagtgcgtg
     7861 gcgcgacagg cctcatgacc gggacgggta atccatctgc ggcaattaat atggttcgaa
     7921 aacacgcgac cagtcgtgaa tttaaaggcg atgtctcggc ggaatacggt agctggaaca
     7981 aagaacggta tgtggcggat ttacaaagcc cactcaccga agacggtaaa atccgcgcac
     8041 gaattgtcgg cggctaccag aataacgact catggctgga ccgctacaac agtgaaaaga
     8101 ccttcttctc cggcattgtc gatgctgatt taggcgatct tacgatgctt tcagccggtt
     8161 acgaatatca gcgtattgat gttaatagcc caacctgggg cggtttaccg cgctggaata
     8221 ctgatggcag cagcaacagt tacgatcgcg cacgcagtac cgcacctgac tgggcgtaca
     8281 acgataaaga gatcaacaag gtctttatga ccctgaagca gcggtttgct gatacctggc
     8341 aagcgacgct gaatgccacc cactctgaag tcgaatttga cagcaaaatg atgtatgtcg
     8401 atgcctatgt aaacaaagcg gatggtatgc tggttgggcc atacagtaat tatggccctg
     8461 gttttgatta tgtcggaggc accggttgga acagcggcaa acgtaaagtt gatgcgctgg
     8521 atctgttcgc tgacggtagt tatgaattgt ttggtcgtca gcacaattta atgtttggtg
     8581 gcagttacag caaacaaaac aatcgttact tcagttcatg ggccaacatt ttcccggatg
     8641 aaattggcag tttctacaac tttaatggca atttcccaca aaccgacagg tcaccacaga
     8701 gtctggcgca ggacgatacc acacatatga aatcgttata tgctgccact cgtgtcaccc
     8761 ttgccgatcc gctgcatctg atcctcggcg cacgttatac caagtggcgg gttgatacgc
     8821 tgacttacag catggagaaa aaccacacca cgccttatgc tggactggtg tttgacatca
     8881 atgataactg gtcgacctac gccagctata cctctatttt tcagccgcaa aatgatcgtg
     8941 acagttcagg caaatatctg gcaccaatca ccggtaacaa ctacgagctg ggtctgaaat
     9001 cggactggat gaatagccgt ctgaccacca cgttagccat cttccgtatt gagcaggata
     9061 atgtcgctca gtccaccggt acacctatcc ccggcagcaa cggcgaaacc gcctataaag
     9121 cggtggatgg gacagtcagt aaaggtgtgg aatttgaact caacggcgca attaccgaca
     9181 actggcagct gacgtttggc gcaacgcgct atattgcaga ggataacgaa ggaaacgccg
     9241 ttaatcctaa tctgccacgc accacggtta aaatgttcac cagctatcgg ttgcctgtca
     9301 tgccagagtt gacggtcggc ggtggtgtta actggcaaaa tcgcgtgtat accgacaccg
     9361 tgacaccgta tggcaccttc cgcgccgagc aaggtagcta cgcgctggtg gatctcttca
     9421 cccgctacca ggtgacgaaa aacttctcgt tacaggggaa cgtcaataac ctgttcgaca
     9481 aaacctacga taccaacgtg gaaggttcta ttgtctacgg cgcaccgcgt aatttaagca
     9541 ttaccggcac gtatcaattc tgaataacgc ctgtaaaaaa ggcagccatc tggctgcctt
     9601 agtctcccca acgtcttacg gattagtggt tacggatgta ctcatccatc tcggttttca
     9661 ggttatcgga tttagtaccg aaaatcgcct gaacaccaga accagcaacc actacgcccg
     9721 ctgcgcccag tttcttcagg ccagcctgat ccactttaga cacatcagca acgctgacgc
     9781 gcagacgggt aatacatgcg tcgaggttag taatgttttc tttaccacca aatgcagcaa
     9841 ccagagccgg tgccatttcg ctggtacctg tcgcttttgc gtcttcagtc gcgtcttcac
     9901 gacccggcgt tttcagatcc agtgctttaa tcagcacgcg gaagatggtg tagtaaacaa
     9961 tggcataacc gataccgacg atcgggaaca gccacagttt gctgctgtta ccagacagaa
    10021 cgatgaagtc gatcagaccg tgcgagaacg acgtaccgtc acgcatcccc agaagaatac
    10081 agattgggaa tgccaggcct gccagaatcg cgtggatgat gtacaggatc ggcgcaacga
    10141 acatgaagga gaactcgatc ggctcggtga taccggtcag gaacgaggtc agcgccgcgg
    10201 agatcataat accgcccact ttcgcgcggt tttctggttt agcagagtgc cagatagcaa
    10261 ttgcggcagc tggcagaccg tacattttga acaggaagcc accagacagt ttacccgcag
    10321 tcgggtcacc cgccatataa cgcggaatgt cgccgtggaa aacctgacct gctgcgttgg
    10381 tgtattcacc aatctgcatc tggaaaggta cgttccagat gtggtgcaga ccaaacggta
    10441 ccaggcaacg ttcgatgaaa ccgtaaatgc caaacgcaac taccgggttc tggtaagcag
    10501 cccactgaga gaaggtctgg attgcagagc caataggcgg ccaaatgaag gacagcacaa
    10561 cgccagtaaa gatggcagtc aggcctgaaa tgatcggcac aaagcgttta ccggcaaaga
    10621 agccaagata ctcaggcagc ttaatacggt agaaacggtt aaacatgtac gctgcgatcg
    10681 caccggagat aatccccccg agtacgccag tatccgccag gtgtttagag gcgatttctt
    10741 cagcaggtaa atgcagtacc agtggcgcaa ccacggccat ggttttaacc atgatgccat
    10801 aggcaacaac tgcggccagc gcggatacgc catcgttatt ggtaaagccg agggcgacac
    10861 cgatcgcaaa aatcagtggc atgtttgcaa agacggaacc gcctgcttct gccataacat
    10921 gcgatacaac ggcgggcagc cagctgaaat tcgcggaacc gacgcccagc agaatacctg
    10981 cgataggcag tacggatacc ggcagcatca gcgatttacc gaccttttgc aggttagcaa
    11041 atgcattctt aaacataatt gagagtgctc ctgagtatgg gtgctttttt tacgttctca
    11101 cgcgtggcaa ggggggagtg cctcgccgtg tacagggcat ctaagcgccc tttatttatt
    11161 acacagagta aaataattca gtgccgattt gtttgacggc tatcacgttt caacttcaga
    11221 tcattggaga agttgcacat tttgacaaaa catatgtcac aatatttcaa acgtggttat
    11281 tcaccgcccg attttcggcg gtgaacttta ctcgttttag ccattaatta cgagctttaa
    11341 aaaaactcat tcaacggatg gattgaaggc gggaagcatc gatgtgaaac agacgggcga
    11401 agttatcggt ggttacctgc gccagttctt caacggcaac acctttcaac acagccatgt
    11461 attctgcaac gtcacgaacc atcgcaggtt gattctcttt tcctcgatgc ggtactggcg
    11521 caaggtaagg tgagtcagtt tccaccagta accgatccag ggggacataa cgcgcagcat
    11581 cgcgcagttg ctccgcatta cggaaggtca caatgccgga aaaggagatg taaaatccga
    11641 gatccagtaa tttacccgcc gtttctctgt cctctgtaaa acagtgtagt acgccaccgc
    11701 aatccgtcac tttttcttcg cgcagaattg ccagcgtatc ggcgcgggcg tcacgggtgt
    11761 ggacgataac cggcttgttc agttcacgac caatctggat atgatggatg aaggactctt
    11821 gctgacgtac tttagtttcc ggcgtgtaat aataatccag cccggtttca cccagcgcta
    11881 caacgccctc ttctgccgcc agacgacgta aatcttctac atcgtaggga tcattctggt
    11941 tgagtggatg cacgccacaa gaaaatacga cgttgtcgcg ttcgcctacc agatcccgca
    12001 tgtgtaaata acccggtaat gttgtggcga ctgccagaca aaatttcaca tcgcgtgcgg
    12061 cggctttcgc cagaacgtca tccacgtcct tatgcaaaga ttcataatcc agaccatcga
    12121 gatggcagtg tgagtcgact aaaaacatga tgtctctctt aaagatgagg aaccggtagc
    12181 acaacgcccg gttgcaggta atgctcaata cgcagtaaaa gatcggtgat gagaagctcg
    12241 cggttgatac ctgtgacaga cattaactgt tcacgaatgt ggcaaacatc ccccagtata
    12301 gcctgcaggc gcgagggaga aagatggttt gccagttcga cgaccagccc cggcacatca
    12361 acattggtca cgtgcgcagc gccataatgg cgttttagcg catccatcag caacgttgcc
    12421 agccagtgta aacgcgccgg agcttgttca ttattaaggg ccgctagcag cgaataccag
    12481 tcgcccgatt gcacgccata tgccagcgcc tgacacaatg tttcacgagc ctgccagtta
    12541 tctccctgaa acaacgccag tgccgcccca ggcgaaccgg cgcttaagcg caatgcggca
    12601 agtagtgcat cctgtgacat tgtcacttcg cgtgaaagcc aggtcacggc gtactgttcc
    12661 ggcggcggcg caaggtaatg taaccgacaa cgactacgta atgttgccag taaacgttct
    12721 ggctcgcggg tagcaaggaa aaaccacgtc tctgctggtg gctcttcaag cgttttcagc
    12781 aatgcgttag ccgcggcgtc ggttagtaag gcagcatcgg ttacccagac aactttcgca
    12841 ccacctaagc gtgcgtgctc attcagcttt tcggtgacct cacgtaccgc atcaatgccc
    12901 agcgcatttt tccctttttc gggagccagg gtgtagtaat cgggatgtgt gccagcctgc
    12961 atcaactgac atccacgaca gtgaccgcaa cttttgtggc cctgcggttg ttggcagagt
    13021 aaataacggc tcagggcgta gattaaagca tcatcgccca tgcccggtaa cgcctgaatg
    13081 agtagcgcat ggtgacctct tccggcctga tagctggcta ccagtttttc gaaatcaggt
    13141 cgtaaccatg gataccatct catgcgtcca actccttcac ccaattggtc acggtagtgc
    13201 ggattgcatc catcacggcc tccagcggct gggtggcatc aatggtatga atgcttttat
    13261 cttgtgctgc cagttccaga tagcgggcgc gggtgcgatt aaagaaatcg aatgactctt
    13321 gctcaatacg atccagctca ccacgggcgc gagcacgttt taagccgacc tctggggtga
    13381 catcaagata aagcgttaag tcagggcgaa aatccccgag aacagcatca cgcagtgttg
    13441 ccagcatatg ttggtcaata ccacgtcctc caccctgata ggcttgagtg gagagatcgt
    13501 ggcgatcgcc aatcacccag gtgccgttag ccagcgcagg tttgatgacc gtttctacca
    13561 gttgaacgcg cgcggcataa aacatcaaaa cttcggcttt atcggtaatg acttcatcgc
    13621 ctaccgattt gatatccagc accaggcttc ttaacttttc ggcaagttgc gtaccgccag
    13681 gttcccgagt gaaaaccatg tcgcggatac ccagttgctc aagcgtctca accaccacgt
    13741 tacgcgcggt agttttgcct gcgccttcca gcccctcaat gacgatatac ttactgcgca
    13801 tttttttcct taagcacttt cagataatcc tgcacagact tgttatgact ggcaagattg
    13861 gtattaaacg tgtgaccacc tttaccatcg gccacaaaat agagatacgg cgtttttgcc
    13921 ggatgcgcag cagccttcag cgaatccgcc cccggcgtcg ctatcgcacc tggcggcaga
    13981 ccggtaatgg tataggtgtt atacgctgtc ggcgtttcca ggtctgcacg agaaagtttg
    14041 ccattataac gctctcccat cccgtaaatc acggtcgggt cggtctgcag gcgcatacca
    14101 atgcgtaaac ggttgataaa tactgaggca acctgatcgc gttcactggc aacggcggtt
    14161 tctttttcga taattgatgc catcgtcacc aactggtttt tatctttata aggcagaccg
    14221 tccgcacgcc cttcccaggc gctatcgacc gctttcacca ttttcttgtg cgctcgtttg
    14281 agtaacgcga catcggtggt attggcggta tacatccagg tgtctggcca gaaccaacct
    14341 tcaatccact ccgggttttc cagttcaagt gcctgcgcta cggtggcgta cttatcgtcg
    14401 ctcagcgtgt gcttgatata cggagcctca cgcaattgct tgaggtagtc gctcagacgc
    14461 atcccttcta ccagtcgcag cgggaactgt gcttctttac cgctttccaa caatttcagc
    14521 atctcgcgca cggtcatctg cggtgtaaag cggtaagtcc cggctttaaa gtgagaaaga
    14581 tccggttcga tacgcagcag ccattggaaa acccgtgggc gattgatgat cttatcggca
    14641 taaagctgtt caccgagcgc cagacgtccg gtccctggct tcagggtaaa tatcgtctct
    14701 tctttgataa gcaatttgct gtcggcaaga tggcgaacct tccagacgcc cacaccagcg
    14761 gcgataccca gtaccaccag caataacaag attatcaata acactttttt catgactaat
    14821 tcgggcgctc acaaagtggg gctaaatatt catataacgt tgctgacgaa aaggagacat
    14881 cgccacaggc acgtacgggc atcactggca ttaacgcatt acaaataacc atctcatctg
    14941 cctgcaacgc ctcttccaga gaggcttgca cttcgacaag ctgataagag gattgtgcca
    15001 gcaaacggat acagaattgt cgcataatgc cgttaacacc tgcctgatcc agtcgcggcg
    15061 tataaactac gttgcccttc cgccagaaca aattagccgc acagcattcc gtaacccacc
    15121 cttcgctgtc aaggaccagc gcctcatcag cgtttgtctg ctcaagatga gagcggatca
    15181 atacttgctc aagccgatta agatgtttaa tacctgcaag atgaggattg cgccccagcc
    15241 gcaccgggct tagcgccaac gtcatccctt cgttacgcaa acggtcgtaa tgtgcaggat
    15301 aagccgtaac ggagagaatc cgcgttgccg gtccactgtt caatgtgctg taccctcgcc
    15361 cgccactacc gcgactgatc acgactttca gtacaccatt ttgctgttct gctgccagcg
    15421 ttttcatctc ttgttcaagc tgaggccaga agtcacagga aatcatcaac cgctgacaag
    15481 catcctgtag tcgctggata tgcgccgata acaaactgac tttaccgtcg ataactctgg
    15541 cggtggtaaa acaaccatca ccaaactgcg ttgcccgatc gcttactgcc agcgattcct
    15601 gcttatgacc gttaattaag aacataccgg ctccttatca gagaattgtt agtgtggcag
    15661 catgttcact acggaacaag tcggaataaa agctaagaaa aaaggcccgc aagcggacct
    15721 tttataaggg tggaaaatga caatttagat ctttttaaag atcaaagaac cattagtgcc
    15781 accgaagccg aaggaattac acagagtgta ttccattccg ctaacctgac gcgcttcgtg
    15841 cggtacgaaa tccaaatcgc aaccttcatc cgggttatcc aggttgatgg ttggcggaac
    15901 agcctgatcg cgcagcgcaa ggatggagta gatagattct actgcacccg ccgcacctaa
    15961 caggtgaccg gtcatagatt tcgtggagct taccaacacg cggcttgcag cttcaccgaa
    16021 gatggttttc accgcctgcg cttcagcttt atcgccagcc ggcgtagaag taccgtgcgc
    16081 gttaacgtag ccaatctgac tcgcttcaat gcctgcatca cgcagagcat ttgccatcgc
    16141 cagagctgcg cctgcgccat tttctggcgg tgacgtcata tgataagcat cgctgctcat
    16201 accaaagccg acgagttcag cgtaaatttt cgcaccgcgt tttttcgcgt gttcgtactc
    16261 ttcaagtacc agcataccgg caccatcgcc cagtacgaaa ccatcacgct ctttatccca
    16321 cgggcggctc gccgcttgcg ggttatcatt gcgggtagat aatgcgcgtg ccgcgccaaa
    16381 accaccaacg cccagcggcg tactggcttt ctctgcgcca cctgcaacca tcacgtcagc
    16441 atcgccatac gcgataatac gcgcagcatg gccaatgttg tgcacgccgg aagtacaggc
    16501 ggtcgcgata gagatgcttg ggccacgcag gccatacatg atagtcagat gacctgccac
    16561 catgttcaca atcgttgacg gaacgaagaa tgggctgatc ttacgtggac caccgttcat
    16621 cagagatgtg tggttttctt cgatcagtcc gaggccgcca atcccggagc caattgcggc
    16681 accaatgcgg gttgcgttct cttccgttat ttcaaggcca gaatcctgca tggcctgaac
    16741 gccagcgaca attccatatt gaatgaaggc atccatcttg cgctgttctt tgcgcgagat
    16801 aatgtcctca cagttaaaat cctttactaa gccagcaaat ttcgttgcat aggcgctagt
    16861 atcgaaatgg tcgattaggc tgatgccact ctgaccggca agcagagctt tccaggtaga
    16921 ctctacggta ttgccgacag gagacaacat gcccagtccg gtcacaacta cacgacgctt
    16981 agacacgttt gtcctccagg gagggaaaaa atgattctag tgggacaaaa agataaaact
    17041 caggcggtcg aacgaccgcc tggagatgtt cacttacgcc tggtggccgt tgatgtaatc
    17101 aatggcagcc tgaacggtgg tgattttctc agcttcttcg tccggaatct cagtatcaaa
    17161 ctcttcttcc agagccatta ccagctcaac ggtgtcaaga gaatccgcgc ccaggtcttc
    17221 aacgaaagaa gcattgttgg taacttcttc ctgcttaacg cccagctgtt cgccgataat
    17281 tttcttaacg cgttcttcga tagtgctcat actcttaaat ttcctatcaa aactcgcttt
    17341 cgcgatggtt ttcgtagtgt ataaaatgtt gaaaaatttg caactaaatc ccggcaggtc
    17401 ttaccacgat tttacgttat tttgaggcct taccccccaa taacgcaaat cattttgcac
    17461 taattattac tcataaccac gcagactgcg cgcaacatga gcattttgtg caaatcgcgg
    17521 tcagaccatg tacatcccgc cgttcacatg caaagtttca cccgtgatgt aagctgcttc
    17581 gtcggatgcc aggaatgcaa ccgcgttggc gatttcctgt gcgccgccga ggcgacccgc
    17641 aggaacctgc gccaggatac ccgcacgctg gtcatcgctc agcgcacgtg tcatgtccgt
    17701 ttcaataaag cccggagcaa caacgtttac agtaataccg cgtgacgcaa cttcgcgcgc
    17761 cagtgattta ctgaagccga tcaagcccgc tttcgccgca gcgtagttgg cctgaccgcc
    17821 atttcccatg gtaccaacca cagaaccgat agtgataata cgaccatgac gctttttcat
    17881 catagcgcgc attaccgctt ttgacagacg gaaaacagat gaaaggttgg tttcgataat
    17941 atcgttccac tcttcatctt tcattcgcat taacaggtta tcacgagtga taccggcatt
    18001 attgaccagg atatccactt caccaaattc tgcgcgaatt ttttccagaa cagattcgat
    18061 agatgccggg tcggtcacat tcaacatcag acctttgccg ttggcaccta aataatcact
    18121 gatcgcctga gcgccatttt cactggtcgc agtgccaata actttcgcgc cacgggctgc
    18181 gagcgtttca gcaattgcgc ggccaattcc gcggcttgca ccggttacca gtgcgatttt
    18241 tccttcaaaa ttcatgattt tcctctttta aagctcgagc gccgctgcca tcgctgaagg
    18301 ttcgttcagc gccgaggcgg tcagggtgtc gacaatgcgt ttcgtcaggc cagtaagcac
    18361 tttgcccggg ccgacttcat agagatgttc tacgccttgc gctgccatgt attcaacaga
    18421 cttcgtccac tgaaccgggt tatacaactg acgtaccagt gcgtcacgaa tggcatcacc
    18481 attggtttcg catttcacat caacgttatt cacaacagga actgttggtg cgttaaaggt
    18541 gattttcgct aattctactg ccagtttgtc ggctgctggt ttcatcagcg cacagtgaga
    18601 cggtacgctc actggtagcg gcagagcgcg ttttgcgccc gcagctttac aagcagcacc
    18661 cgcacgctca accgcttctt tatgaccggc aataaccacc tgtcccggag agttaaagtt
    18721 taccggagaa acgacctgac cttctgcagc ttcttcacac gctttcgcaa tagacgcatc
    18781 atccagaccg atgattgccg ccatagcacc cgttccttcc ggtacggctt cttgcatgaa
    18841 cttgccgcgc atctcaacca gacgcaccgc atcagcgaaa tcaatcacac cagcgcaaac
    18901 cagcgcggag tattccccca ggctgtgacc ggccatcatt gccggtgctt taccgccctg
    18961 ctgctgccat acgcgataca gcgcaacaga tgcagtcaac agcgctggct gggtttgcca
    19021 ggttttattc agttcttcag ctggcccctg ctgggtcagc gcccacaggt cgtagcccag
    19081 cgccgcagaa gcttcagcaa acgtttcttc gacaattgga tagctcgccg ccatatcagc
    19141 cagcattcca acggtttgag aaccctgtcc agggaacaca aatgcaaatt gcgtcatgtt
    19201 ttaatcctta tcctagaaac gaaccagcgc ggagccccag gtgaatccac cgccaaaggc
    19261 ttcaagcaga accaactgcc ccggcttaat gcgcccgtcg cgtacagctt catccagagc
    19321 gcacgggaca gaggccgcag aggtattacc gtggcgatcc agcgtcacca cgacattgtc
    19381 catcgacatg ccgagttttt tcgctgttgc actgataata cgcaggttag cctgatgcgg
    19441 aaccagccag tccagttgag aacggtcaag attattcgcc gtcagcgtct catcaacgat
    19501 gtgcgccagt tctgttaccg caaccttgaa gacttcgttg cccgccatcg tcagatgaat
    19561 tgaattctct ggattcacac ggtcagcatt aggcagcgtc agcaactcac catagctacc
    19621 gtcggcatgc agatgggtgg agatgattcc cggctcttca gaggcagcca gcaccgcagc
    19681 gcccgcgcca tcgccaaaaa taataatagt cccacgatct gttggatcgc aggtgcgcgc
    19741 cagtacatcg gaaccgacga ccagagcata cttcaccgcc ccagatttca cgtattgatc
    19801 ggctacgctt aatgcatagg tgaaacctgc gcaggctgct gcaacgtcaa atgccgggca
    19861 acctttaatg cccagcatgc tttgaatctg acaagctgcg ctcgggaaag cgtgcgtagc
    19921 agaagtcgtt gccacaacga tcaggccaat ctggtctttc tcaatgcccg ccatctcaat
    19981 tgcgcgtgtc gccgcttcaa agcccatggt tgaaacggtt tcgtttggcg cggcaatgtg
    20041 gcgttcgcgg ataccggtac gagtgacaat ccactcgtca gaggtgtcca ccattttttc
    20101 caaatcggcg tttgtccgca cttgttcggg cagatagctg ccagtaccaa taatcttcgt
    20161 atacatgtac gctcagtcac ttttcggtta tataccgtca cttgcaaact gcgagttcgc
    20221 tggcagcgtc ctgctaccgc agagttccgc ttttgccacc gtccagcagc tcaaaaccag
    20281 ctgggtatac agattccagg cgagcggcaa ttcgctgagg aacttgtcgc tgcaccgcct
    20341 gcactgcctg ttcaatcgcg accgcaaaag ctcgctgatt ggctgcacca tgacttttta
    20401 tcaccgtgcc gcgcaatcct aacagacagg cgccgttata ctggtcgggg ttgaggtgac
    20461 tgaatcgcct cgtcaggctc ttttgtagcc aacgctttaa taacagtagc caccacgacc
    20521 gttttttccc ttcaccctga gatttcagca gagaaaggaa catcctgaca acaccttcca
    20581 tcgtctttaa tgtgacattt cctgtaaagc cgtcacaaac cagcacatct gtcttgccag
    20641 ttaacaactc attggcttca agatagccga tataattgat agaagggatt gttttaagca
    20701 ccgctgaggc atcccgaata ctgtcgagac cctttacttc ttcttcacca atattgagca
    20761 acgccacgcg aggattagga atttccacca cctcttcagc cagaactgag cccataatgg
    20821 caaattgcac caacattgta ctgtcacaat cgacgttggc ccctaaatcg aggaccaccg
    20881 ttttgccctt ttgctgatgc ggtaataccg tcaccagcgc cggacgctca atcccctcca
    20941 ggggcttgag taataatttt gccagcccca ttagcgcccc ggtattaccg gcactgacac
    21001 aggcttgcgc tcgaccttct ttcaccagct ccagggccac gcgcattgaa ctcccacgac
    21061 tggcgcggat agcttgcgaa ggccgggcat cactggcgat aactgactgc gcaggaataa
    21121 tctgcagacg cgaacgttgt tcaaagtcag ctttagcaag taatggcgtg atggcgtcgg
    21181 gattgccgac taaaagaaga gtgagttgcg aattagaatt cagtgcctgc aatgctgcag
    21241 gcactgtcac ggaagggcca aaatcccctc ccatgacatc taacgccagg gttagacgtg
    21301 tcaaggtatc gtcgccgccc ggtttggtct ttccccagtt gcctggggaa aatcctcact
    21361 aagcttcatc acgcagacgc gtaattactt agcgatgacc ttgcggccgc ggtagtaacc
    21421 gtcggcagtg atgtggtgac gcaggtgttt ttcaccagaa gttttgtcta cagacaggct
    21481 ggtgactgcg gtcagcgcgt catgggaacg acgcatgcca cgtttggaac gggttggttt
    21541 attctgttgt acggccatgg accttactcc tcaattacgg tttatccccg atccaacggg
    21601 gagcaccaat tacttacgct ttaagctggc taatacggca aatgggtttg gcttttgcgc
    21661 ttcttcaggc agttcaccaa agaccatgtc cgcttcggac acttcacagt gttcagaatc
    21721 atgcaccgga actaccggca aggcgaggat gatttcatct tcaaccattg caagcagatc
    21781 gatttcaccg aattcgttaa cctcaatcgg ttcatacgct tccggcagtg cttcagcctg
    21841 ttcgtctgaa cgcacaggac taaaacaata cgttgtgtag acctgatgag taaacggctt
    21901 cccgcaacgc tgacactcga gcgttaccgt caccttcgca tcgccgttta acactgcgag
    21961 acgttggtta tcgatagcga acgacatgga gcattccaca tcactgtcca cactgactac
    22021 ggattcggcg acgcgctcaa cctgatcagg agtatagata ccctggtaat caaggcgttt
    22081 ttgagccgta cgaaccggat cgagagtcag gggtaatttt accttttgca tagggcgcgc
    22141 atattaactt tgtaacgtca tagagtcaaa gaaaaaggca gcctgtggtt gccttttgcc
    22201 aataattcgc acacattgcg ggttactgct ttattttcgt tcagtgctga taacgaacat
    22261 caacctgcaa aacgaagcgc atagtttaaa atggctatca tcaatacgct atatctgatg
    22321 gaaaaaaata tgcctgaact tattttagcc tccacctcgc cctggcgtcg cgctcttctg
    22381 gaaaaactgc aaatctcttt tgaatgtgca gcacccgagg tcgacgaaac tccacgcagc
    22441 gacgaatcac cgcgacagtt ggtgcttcga ctggcacaag aaaaagcgca atctctggcg
    22501 tcacgttatc cggatcattt aattattggt tcagaccagg tatgtgttct tgatggcgaa
    22561 atcaccggta aaccgttaac ggaagaaaat gcccgtctgc aattacgcaa agccagcggc
    22621 aatatcgtca ccttctatac tggactggcg ttgtttaatt cggcgaatgg gcatctacaa
    22681 acagaagtgg agccttttga cgtccatttc cgtcacctga gcgaggcgga gattgataat
    22741 tacgtgcgta aagagcatcc cctgcactgc gcgggtagct ttaagagtga aggatttggc
    22801 attacgctgt ttgagcgttt agaggggcgt gatcctaaca cgctggttgg tttaccgcta
    22861 atcgcgctgt gtcagatgtt acgtcgggaa ggaaaaaacc cgctgatggg atagtttttt
    22921 gcgccggatg cggttacgtc gcatccggca atcggtgctg gcggcctgat gcggcataaa
    22981 cgccttatca ggccaaccgc ccattatcat gcttatatgc tcgtattagc gcgcgttacg
    23041 cagtttttgc aaacaacgct tcaaaccatc atccatcggc gcttcgatac gcatcacctc
    23101 accggtcccc ggatgggtaa acttcaacgc tgcggcgtgc aggaacagac gatttaatcc
    23161 cgtgcctgct tcagtgagct gtctgtcaaa ttcacggtca ccgtagcgat cgtcaaaggc
    23221 aatcggatga cccgcatatt gtgtatgcac acggatctga tgagtacgcc ctgttaccgg
    23281 gctacaacgc accagggtgg caaatgcata gcgctcttcc actttaaagc gtgtttccga
    23341 cggtttgcct tcctgactca cacgcacgat acgttcgccg ctttgcagaa tatttttcag
    23401 taacggcgct tgaacgctct tcacatgcga ctgccactga ccgcgcacca gcgccaggta
    23461 atctttttgc atcccttttt cacgtaattg ctcatgcaga gaacgcaacg ccgagcgttt
    23521 tttcgctacc agcaacacac ctgaggtgtc ccggtcaaga cgatgaacca gttcaaggaa
    23581 ccgcgcttcc gggcgcaacg cccgcaaacc ttcgataacg ccgaagctta aaccactacc
    23641 gccatgtacc gccgtaccgg aaggtttatt cagcaccagg atatggtcat cttcatataa
    23701 gatgacgtcc gccagcgccg ccaccttttg cagatgtggc gaaaccgcct cttcttcccg
    23761 ttcagcaacg cgaaccggtg ggatacgcac ctcatcaccc gcttcgagtt tatattcagg
    23821 cttaatacgt tttttgttca cccgcacttc gcctttacgc aaaatacggt aaatcatact
    23881 ttttggtaca cctttcaatt gggtacgcaa aaagttatcg atacgttgcc ccgcttcgtc
    23941 agcggtgata gcaacaattt ttacggatgg agtctctgtt ttcatggtgg gcgattctaa
    24001 ttagccaaca ggattcgcgc cactcatttt tctatgctta tatttagttt tgcaccttat
    24061 tacttcacta catgatcact ttattgatgg ttattaaacc aatcaccagc aagaagtgaa
    24121 aaaactgtga gtaagcgggt gataaatggt aaaagtcatc ttgctataac aaggcttgca
    24181 gtggaataat gaggccgttt ccgtgtccat ccttgttaaa acaagaaatt ttacggaata
    24241 acccattttg cccgaccgat catccacgca gcaatggcgt aagacgtatt gatctttcag
    24301 gcagttagcg ggctgcgggt tgcagtcctt accggtagat ggaaatattt ctggagagta
    24361 atacccagtc tgtttctttg ataattgcgc tgtttttccg catgaaaaac gggcaaccga
    24421 cactctgcgc ctctttgagc tgacgataac cgtgaggttg gcgacgcgac tagacacgag
    24481 gccatcggtt cacacccgga aaggcgttac tttgcccgca gcttagtcgt caatgtaaga
    24541 ataatgagta agttacgatg aaaagaatgt taatcaacgc aactcagcag gaagagttgc
    24601 gcgttgccct tgtagatggg cagcgtctgt atgacctgga tatcgaaagt ccagggcacg
    24661 agcagaaaaa ggcaaacatc tacaaaggta aaatcacccg cattgaaccg agtctggaag
    24721 ctgcttttgt tgattacggc gctgaacgtc acggtttcct cccactaaaa gaaattgccc
    24781 gcgaatattt ccctgctaac tacagtgctc atggtcgtcc caacattaaa gatgtgttgc
    24841 gtgaaggtca ggaagtcatt gttcagatcg ataaagaaga gcgcggcaac aaaggcgcgg
    24901 cattaaccac ctttatcagt ctggcgggta gctatctggt tctgatgccg aacaacccgc
    24961 gcgcgggtgg tatttctcgt cgtatcgaag gcgacgaccg taccgaatta aaagaagcac
    25021 tggcaagcct tgaactgccg gaaggcatgg ggcttatcgt gcgcaccgct ggcgtcggca
    25081 aatctgctga ggcgctgcaa tgggatttaa gcttccgtct gaaacactgg gaagccatca
    25141 aaaaagccgc tgaaagccgc ccggctccgt tcctgattca tcaggagagc aacgtaatcg
    25201 ttcgcgcatt ccgcgattac ttacgccagg acatcggcga aatccttatc gataacccga
    25261 aagtgctcga actggcacgt cagcatatcg ctgcattagg ccgcccggat ttcagcagca
    25321 aaatcaaact gtacaccggc gagatcccgc tgttcagcca ctaccagatc gagtcgcaga
    25381 tcgagtccgc cttccagcgt gaagttcgtc tgccatccgg tggttccatt gttatcgaca
    25441 gcaccgaagc gttaacggcc atcgacatca actccgcacg cgcgacccgc ggcggcgata
    25501 tcgaagaaac cgcgtttaac accaacctcg aagctgccga tgagattgct cgtcagttgc
    25561 gcctgcgtga cctcggcggc ctgattgtta tcgacttcat cgacatgacg ccagtacgcc
    25621 accagcgtgc ggtagaaaac cgtctgcgtg aagcggtgcg tcaggaccgg gcgcgtattc
    25681 aaatcagcca tatttctcgc tttggcctgc tggaaatgtc ccgtcagcgc ctgagcccat
    25741 cactgggtga atccagccat cacgtctgcc cgcgctgctc cggtaccggt accgttcgtg
    25801 acaacgaatc gctgtcgctc tctattctgc gtctgatcga agaagaagcg ctgaaagaga
    25861 acacccagga agttcacgcc attgttcctg tgccaattgc ttcttacctg ctgaatgaaa
    25921 aacgttctgc ggtaaatgcg attgaaactc gtcaggacgg cgtacgctgt gtgattgtgc
    25981 caaacgatca gatggaaacc ccgcactacc atgtgctgcg cgtgcgcaaa ggggaagaaa
    26041 ccccaacctt aagctacatg ctgccgaagc tgcatgaaga agcgatggcg ctgccgtctg
    26101 aagaagagtt cgctgaacgt aagcgtccgg aacaacctgc gctggcaacc tttgccatgc
    26161 cggatgtgcc gccagcacca acgccagctg aacctgccgc gcctgtcgta gccccagcac
    26221 ctaaatctgc accggcaaca ccagccgctc ctgcccaacc tgggctgttg agccgcttct
    26281 tcggcgcact gaaagcgctg ttcagcggtg gtgaagaaac caaaccgtcc gagcaaccaa
    26341 caccgaaagc agaagcgaaa ccggaacgtc aacaggatcg tcgcaagcct cgtcagaaca
    26401 accgccgtga ccgtaatgag cgccgcgacc ctcgtagtga acgtactgaa ggcagcgata
    26461 atcgcgaaga aaaccgtcgt aatcgtcgcc aggcacagca gcagactgcc gagacgcgtg
    26521 agagccgtca gcaggcagag gtaacggaaa aagcgcgtac caccgacgag cagcaaacgc
    26581 cgcgtcgtga acgtagccgc cgccgtaacg atgataaacg tcaggcgcaa caagaagtga
    26641 aggcgctgaa tgttgaagag caatctgttc aggaaaccga acaggaagaa cgtgtacgtc
    26701 cggttcagcc gcgtcgtaaa cagcgtcagc tcaatcagaa agtgcgttac gagcaaagcg
    26761 tagccgaaga agcggtagtc gcaccggtgg ttgaagaaac tgtcgcagcc gaaccaattg
    26821 ttcaggaagc ggcagcccca cgcacagaat tggtgaaagt tccgctgcca gtcgtagcgc
    26881 aaactgcacc agaacagcaa gaagagaaca atgctgataa ccgtgacaac ggtggcatgc
    26941 cgcgtcgttc tcgccgctcg cctcgtcacc tgcgcgtaag tggtcagcgt cgtcgtcgct
    27001 atcgtgacga gcgttatcca acccagtcgc caatgccgtt gaccgtagcg tgcgcgtctc
    27061 cggaactggc ctctggcaaa gtctggatcc gctatccaat tgtacgtccg caagatgtac
    27121 aggttgaaga gcagcgcgaa caggaagaag tacaagtgca gccgatggtg actgaggtcc
    27181 ctgtcgccgc cgctgccgaa cctgttgtta gcgcgccagt tgttgaagaa gtggctgaag
    27241 tcgtagaagc ccccgttcag gttgccgaac cgcaaccgga agtggttgaa acgacgcatc
    27301 ctgaagtgat tgctgccgcg gtaactgaac agccgcaggt gattaccgag tctgatgttg
    27361 ccgtagccca ggaagttgca gaacacgcag aaccggtggt tgaaccgcag gaagagacgg
    27421 cagatattga agaagttgcc gaaactgctg aggttgtggt tgctgaacct gaagttgttg
    27481 ctcaacctgc cgcgccagtc gtcgctgaag tcacagcaga agttgaaacg gtagctgcgg
    27541 ttgaacctga ggtcaccgtt gagcataacc acgctaccgc gccgatgacg cgcgctccgg
    27601 caccggaata tgttccggaa gcaccgcgtc acagtgactg gcagcgccct acttttgcct
    27661 tcgaaggtaa aggtgccgca ggtggtcata cggcaacgca tcatgcatct gccgctcctg
    27721 cgcgtccgca acctgttgag taatagttag ctcaagtaat caagccctgg taactgccag
    27781 ggctttttta tttcatcttt gagtcaacat attaaaattc atcaaatggc atccttgcta
    27841 accaacaata caaaataggc aaaaaaatgc ccgctccggc gatcccagag cgggcagaca
    27901 aaacataccc agtttcatga tatgttttaa agcgagctta tttgttgagc tggaagagcg
    27961 acaatccctg catatcggta aatgctttat acgatgcctg caatgccgtt tgctgcatga
    28021 tgtaagatga aatagttgca ttccagtcca catcaaccag atcgctcatc tgctgcgttt
    28081 gccctaaagc gcgatcgcta cctaatgaat ccagcgactc cagttcgttc agttgcgtgc
    28141 ctaattccgc gcgaacagtc agcacattgt tcagcgagtt tttcagtccg cggttggttt
    28201 tatccagtgc cgcagcggcg gtttctttat ccgcttcgct atccgcgacc ggcgttttca
    28261 gggctgcgat ggcgctatcc agcatggcaa aaagattggt ttcagaagcg ctaccgtctg
    28321 gttccgctac cgcgttgcta gtaatactgt cgaaaatttt gtcgcccgta tgtcctatca
    28381 ccatcgaacg cgaagcatcg acctgttgtt taatactttc tgctccgcct tcgtatttgc
    28441 catccgcctc gctaaacggt gcggattctg ttttataacc ggcaaaaatg tagcgcccgt
    28501 taccgtcagt ggtgttcgcc agattcagca actggtcacg aagcccctga atatccgtag
    28561 ccagcgaggc ccggtcatcg tcactcaagg tgccattgct ggcgtagaca attttttcct
    28621 gagcattctg tatagcggtt gtgacctggc taagtacact ctcttccagt gacacttttt
    28681 gagtggcgaa agtacgcgcc agcgtgtact ggctgttttg cgcctgtgcc tgggagagaa
    28741 caacagcttg tgatgcagca atgggatcat ctgaagggtt aacgactcgc ttacccgtcg
    28801 acatctgttc gccgtacttc atccattctg cctgagaatt ggtgatgccg cgcatgtttt
    28861 gctggtacat catctgggta ctgaaacgca ttttatctcc ccttagcgaa tgttaatcag
    28921 cgcatcaaaa atcgcgtttg ccgtctgcag aacctgcgca ttcgcgaggt aatactgctg
    28981 aaaacgttgc agatttccgt actcctcatc gagattgaca ccggaaatcg actgctgctg
    29041 attggaaagc tgcgtcacca cattaccttg agtggtgcta ctggttttca acgtcgcggt
    29101 tttattaccg atatcactca ctaacgaggc ataagcgtcg ttaaaggatt tcgcaccgcc
    29161 caccgtttta ctgttgcttt gcagatcgag cagggcctga ccgttgcggt tgtcgctatc
    29221 acccgcatct tctgcgctcg ccatggcgat tttcgcttcg tcggtgatta atacatccat
    29281 gttgacgatg gcgtcactta ccggtttcag cgtgaagctg tcgttaacgg caggcgttcc
    29341 tgtaaacgtc aactccagac catcaaatgc cactttaccg ttggcatccg gcgtcaccgt
    29401 aaaagtggta ttgctggcaa ggcgggtgac ctgccactga ttattatcga acgagatttt
    29461 gtaatctgtc gccagtaccg cggaggtatc agttaccgtt gcaccgatcg caacgtcacc
    29521 tttgtttttc gtgttttgca gaaccgcggg cttaccgata gcaaagaaat cttcaccggc
    29581 atcgccgtta gcatcaaatc cggctttgtg ttgggtgttg aaagcctcgg caaatgccag
    29641 cgccagttgt ccaagcgtat tacgcgtctg gtccagatcc tgagaacgga atgtcagaat
    29701 gccgcccagc gacccggtat tcagtaattt ctccgggatc tcaatattgc ctgccgtccc
    29761 atcaacataa gcgacagtcg tacgagaagg gtcggcgctg gaaggaactg ccgcaagttg
    29821 ccgcgccgta cttccctgaa ccagtgagta gccattggcc atcgtgatgt tataagtgcc
    29881 accatcctga acactgactt ctacgccaac aatctggttt aattcgctca ccagttgatc
    29941 gcgttgatcc agcagattgt taggtgacgc ccctgccccc acgcctgtca ggcgggagat
    30001 ctgatcgttc aggctggcga tttgtttagc gtaattgttg atctgatcaa cactggcacc
    30061 tatcgcgata ttgacctgtt tgtcctggtc gcgcagatat tgatcggtgg ttttaaactg
    30121 attcaccaac ccttctgatt tcccaatcag cgcctggcgc gctgccggat cttccgcgtt
    30181 actcaccagc gtttgcaggc tggtgaagaa atcctgcatc tgtgttgcca gcgaagaggt
    30241 actggtggag agcatattgt cgattttcga catctgctca tagcgggcag tcaggccgct
    30301 actttgcgtc tgcgccgcac gtaactggtt ggtaataaac gcatcatact cacgctgcac
    30361 accagaaacg tagacgccat tgccaaccca gccgccagcg cccaacgtgc tattggcctg
    30421 cgccataata gtggtttggc gggtatatcc ggcaacgtta tagctggaga tattattact
    30481 tgccgtattt aacgccgcct gggccgcgtt cagtccgctc atggcgttat tgatcaagct
    30541 ggacatggag gttccttata agccttcaat tacagagtat tatcggcagc gacccgccgg
    30601 acttgagtta ttcagaacag attatcaatg ttcatgctgt aggttttgct caccttgtcg
    30661 cttatcgatt tcatctgctg aatcatgttg gtgagtttgc gggcatagtg aggatcggtg
    30721 gcatagcccg cgtcctgtag ggcctgcgcc ccctgctccg cactcgcggc ggtcgtcacg
    30781 gcggcgtagc gcgggttacg cgttaacagc ccaacgtaat ccgacaatgc ttccagatac
    30841 gagctgtaga cccgaaactt cgcttttact ttcttcgctt cgccattttc atattcagtc
    30901 gtggtgattt cagtgactgg ccctttccag ttgccagagg ctttgacacc aaacaggtta
    30961 tagctcggct cgccgttttc acggcggatt tgccgttgtc cccagccaga ttccagcgcc
    31021 gcctgagcga ggatcaaatg atgtggcaca ccactttgct ggcttgccag ttgggcgggc
    31081 agcgagagtt gcgcgaggaa tgctttactg tcacccggca gcgaatcatc gtagttacgt
    31141 ggtacggcct tttgcaccag ctgcgaaagc gtctgatttt gataacgcac cacggtttcg
    31201 agcgggaatt ttatcggtgc tgctggcgtg gactcctctg gcaatggttg ttctggcgtc
    31261 atctgtttaa ccatcatctc tgccagcccc agacctttgc ccgccgtcat ctgttgggca
    31321 atctgctggt catacatact ggtatacagg cgagtgtgct cgctgctgaa caggccatct
    31381 tttggtaaag cgtcgcgcat gcttttcaac atcatctgca cgaacatccc ttccacctgg
    31441 cgggccaccg gacggatatt tgccgctgga tcttcgcccg ctttcgcctt tagttcgttg
    31501 agtgattgcg catcccaggc cgcacttgcc agtagtttgc tgtcgctgat catcagatga
    31561 tttccagttt tgcccgcaga catcccgcac tttgcattga ttgcagtatg gacatcagat
    31621 ccatcggcgt agcgcccagc gcattgagcg cacgcaccac gttattgagg ctggcgctgg
    31681 aacgtacgct ttgcagcgaa ccgccgctct ggcgtaaatc gatctgcgtt tgtggagtaa
    31741 ccacagtctg cccaccacca aacggtgtat ctggctggct gacattggcc tgacgattaa
    31801 ctgttactga gagattcccc tgcgctaccg cgcagctgtc gagggtcact tcgcgattca
    31861 tcaccaccga accagtgcgc gagttaatca ctactttagc gtcctgcggg gtgacattaa
    31921 cctgcatatt ctggatatcg gcaaggaaac ggacctggga actgttgcca cttggtacgc
    31981 gcacctgaat agtccgcgca tctaacgcgg tggcgctgcc atatccacgc acgcggttga
    32041 tggtgtcagc gatttgctgc gccatactga aatcttcgtc gttaagttgc aaattaaggg
    32101 tattcccgac gccaaactgg ctgggcaatt cacgttcaat aaccgcacca ttggtgatcc
    32161 gtccaccgtt cagttgattc acctgaacac tgctaccgcc agcggaggcc cctgcgccgc
    32221 caaccagaat attgccctgc gccagcgcat acacctgact gtcaacgccc ttaagcggtg
    32281 tcatcaataa cgtaccgcca cgcagacttt tggcatttcc catggaagaa accaccacgt
    32341 caatggtttg cccctgacgt ccaaacggtg gaagtgacgc tgtcaccata accgcagcga
    32401 cgttttttag ctgcatatta gtgcccgtcg gaacggtaat tcccagctgt gagagcatgt
    32461 tattaagcgt ttgtgtggta aacggcgtct gagttgtctg gtcacctgta ccatcaagcc
    32521 ccaccaccag gccatagcca atcagtgagt tttgccttac cccctgaaca ctggtgagat
    32581 cgcgaatacg ctcagcctga gccgccgtgg taaccagtaa aagaattaat gcagagagaa
    32641 atttaatcac cacagcctca cttacattgg cgacaggtta aggaagaaac gctgcaacca
    32701 gcccatattt tgcgcttcgt taatgtagcc attgcctacg tattcaatgc gcgcatctgc
    32761 cacctgagta gacggtacgg tattgctgcc gctgatagtg cgtggattaa ccaccccaga
    32821 gaagcgaata aattcggtac cctgattaat ggcgatctgt ttttcaccca ccacatgcag
    32881 gttgccgttg accagtacct ggtcaaccgt caccgtcaac gtaccgctaa aggtattgct
    32941 ggcattggcc ccgccttttc cgttgaacgt gttaccaccg gaggcttcga catcggcacg
    33001 agcgttacca aacaacccct gcaaatagcg cggcacagta tcaaagccaa aattagtttt
    33061 accgtcacgg ctggcattcg cagaggagct tttgctggcg ctgacgttct cctgcaacac
    33121 gatagtcagc gtatcgccaa tattgcgtgg tcgacgatct tcaaacagcg gttgatagcc
    33181 atagttaatc ggctgagcag actggaaaat agacccgttg gcgacgggcg tcggaccggg
    33241 aaccggttgt gcactggtcg ccccctgtac cagcggcgtg gagggtatcc aggcgcagcc
    33301 ggttagtgaa agcgccaaca agctggaaat ggcataagta tgcgcagcgt ttttttgcat
    33361 ggctatcatc ttcaaaaatc agtaaaccgg tgaacctgcc caccggttaa gccttagagt
    33421 tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa
    33481 gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt agacgtttca
    33541 acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc gttcggtgca
    33601 ccggaggatt gcgtttcggt gtagaggttt tcgccaatgc tctccagtcc ggtgtcattc
    33661 ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt
    33721 acgctgacca cgccatcacg accgatggtg atacttaacg cattcgccgg aatggtgatc
    33781 gctggctgca cctgaaaacc accggccgtc accagctgcc cgttctgatc cacctggaaa
    33841 gagccgtcac gggtataggc tgatgagcca tctggcaaca tcacctggaa aaagccctgc
    33901 cctttaatcg cgacgtcttt actgttattg gtttgcgaca ggttgccctg gctgtgtaaa
    33961 cgttccgtgg cgacagggcg tacccccgta ccgatttgta atccggatgg taaggtggtt
    34021 tgttcggaag actgtgcccc cggctggcga atggtttgat aaagtagatc ttcaaacacc
    34081 gcgcgctgac gcttaaaacc gttggtacta acgtttgcca ggttgttggc aatgacgtcc
    34141 atattggttt gctgggcgtc aaggcccgtt ttggcgatcc ataatgaact gatcataaaa
    34201 tgtcctgtag tggattagtg acaaagtgca tatgtcttct tgccggatgc ggcgtaaacg
    34261 ccttatccgg cctacaagag ctcgcaaaat cgataaattg cgattgcccc catagacccg
    34321 ataaacttgc gcatagggca acttatactt gtcatgtatc ctttcaatta actcatcgac
    34381 agcagttggt tggcacggcc tgcgttatca tcgacgctgc tgatcacctt catctgcatt
    34441 tcaaaacgcc gtgcgctggc aatcatgtcg ctcattgccg caacggcatt gacgttactg
    34501 ccttccagaa cccccgacat cacacgcaag gttggatctg cctgcagtac cggcccacgc
    34561 gtagcctggc tttctgcgct taaacgaaaa atgccatcgt caccgcgctg gacttcgctg
    34621 cccgtggctt tcaccagctt cagacgcccc actggcgcaa ccgtatttgc cggatcgccc
    34681 ggattcagcg ccgagattgt gccatcggca gcaatagtga tttccgcccc ttccggcaca
    34741 gcaattggcc ccgcctcgcc taccaccgga tgtccctgaa ttgtcagttg cccggtggga
    34801 tcaacctgaa tgctgccatt acgcgtatac ccttcactgc cgtcagcggt ctgcacgacc
    34861 agccaaccat cctgctgcaa cgcaacgtcc agcgggcgcg aggtgtaatc catcttgccg
    34921 ggcgtcatgt ctgcacccgg tgttgacgcc gttaccagcg tgcgcgtggg cagtgaaagc
    34981 ccttccaccg gcactgcgcg caaggcgttt aactgcgcgc gaaaaccggg cgtagaggca
    35041 ttggccagat tactggcggt taccgcctgt tgattcagcg tctggctggc tgctcccatc
    35101 gcggtataaa ttgcgtgatc cattgagcta tcccgtcagc gattagcgta agttaaccag
    35161 cgtattgagg atctggtcct gggttttgat ggtctgggcg ttagactgat agttacgctg
    35221 ggcaacgatc atattgacca gttctttact gagatcgacg ttggacgctt ccagcgcacc
    35281 gttggtcagg gtgccaaagt tgcccgtccc ggctgtcccc aacagcgcca cgccagaaga
    35341 ttgcgtagcg gaccagacgt tgtcgccttc ggatgccaga ccttcgttgt tggcaaagtt
    35401 cgccagtaca atctgcccca gcagttgggt ttgttcgttg gaatagttgc cgacaaccgt
    35461 accgtcatca ttgatttgat aactcaccag atcgcccggt ttgtagccgt tctgggtggt
    35521 tgccacaatg ttgttagcgc cggtattttg ctgcatggag ttgaggaagc tcaggctaaa
    35581 cgtggcggga tctgcgccgt taattgcgcc ggtggtaata tcctttgttg gatctgaggt
    35641 cagaacgcca ttggcattaa acaccagcgt cgttgcaggg ttagctgtgc ctgttggatc
    35701 actgctatcc agggtgtaaa catcccagtt attatccccg gtcttcacaa agtagacgct
    35761 catgtcatga gcattaccct gactatcgaa aacagtcacc gaaccttttt tgttatagct
    35821 atccgcattg ctggcatcaa atgcgttaac agagggaagc gaatcactgg aattcaggtt
    35881 gatctgcatc gacgccgtgg tggtagtttt cgctgccatc agggtattcg gaatcgaaat
    35941 attggtcgga ttcgcccctt gctgaatagt cggcggcgta ccggttgccg ggtaacccgt
    36001 cagctgtaaa ccttgcatat tcaccaggtt acggttttca tccagcttaa attgtccgtt
    36061 acggctgtag aataccgaac cattgctgtc taccagacgg aaaaaaccgt tctggctgat
    36121 agcaacgtcc agaccacgcc cggtgttggt ggtcgtgcca tcggtaaagt cctgagtgat
    36181 accagcaact tttaccccca gtcccacttt cgaaccggca aacatatcgg caaaagaggc
    36241 cgtgcctgat ttaaagccgt aggtggcgga gttggcgata ttgttgccaa taacatcgag
    36301 gttggtggcg gcagcgttta atccgctaac cgcttgagaa aaggccatga ctgactcctg
    36361 aaatgtgaag gcttaaatta tctgccgtac ttcgtcgagg gtagtggtgc cgtaagtgcc
    36421 aagatccagc gtattaccgt tgttgccgcg gatcaccccc tgcaccagag caaactgcag
    36481 cggttgggca accagttgtg taccaccgtt actggcgcta atcgctacat tgtaagaacc
    36541 gttcggcgca gtgctgccgt cagtcaacgt accgtcccag gtgaaactgt ggactccggc
    36601 agtcagttca ccgatatcaa tggtacggac gaccgcgccg tttttatcgg tgatggtggc
    36661 cgtcactttg tctgctgcct gttgcaactc aacaccaaac ggcgtggtcg tggtcacagc
    36721 cccttcttca ctgccggttc ccgcaagaac tgtggtaccg gggatcatca cgccgtgacc
    36781 gataaggtta ctggcctgta acgactgact gttatcaatc tgtccggaaa tagatccgag
    36841 cgtggtattg agtttttcaa tcccactgac cgtgctgatt tgtgccaatt gcgacgtcag
    36901 ctcgttgttt tccattggat tggtcgggtc ctggtttttc agctgcgcca ccaacaaagt
    36961 cagaaaactg ctttgtaaat ctgcggcgtt gctgcccgtg agcgaactac tgctggtggt
    37021 actgacgccg gtattcgtcg gatcggtggt ggttaccgca atagacatag ctttctcctt
    37081 tattgaccga gcgtaagggt tttcagcatc atgcttttca ccgtgttgag cacttcaaca
    37141 ttggcctgat aactgcgtga cgccgacatg gtgttaacca tctccccgac gacatcaacg
    37201 tttggcattt tcacgtagcc ctttgcatcc gccagcggat tacccggttc ataaaccagt
    37261 ttgtccgggg cctgactttg tataacatcg gcaaccttta cgccgcctgt cgcagcacct
    37321 ggtgcagcgt taacctggaa taccacctgt tttgcccgat atggctgtcc atcgggaccg
    37381 gtcacgctat cagcattcgc cagattactg gccgccacgt tcagacgctg ggactgggca
    37441 gttaacgccg acccggcgat atcaaaaata ttcagcagtg ccatccgtta atttccgctc
    37501 tgtaaaacgt tcatcatgcc tttaatttgc ccgctcaacg cgctaaggct catctggtat
    37561 tgcaggctgt tatcggcaaa ctgggtgcgt tcgcgatcca tatcgacagt attaccgtca
    37621 agcgaaggct ggtccggaat acggtattgc agttctgcgg aaggaggcgt cagcgcctgc
    37681 gccggaatgt gttgcgttga ggtcatcgtc agagcaacca cactggttgc atcccgtcca
    37741 cgttgcatga cttttttaag ttcactggca aaatcgatat cgcgcgcctg ataaccaggg
    37801 gtatcggcat tggcgatgtt tgctgccagc acttcctgac gctgggcgcg cagattgagc
    37861 gcctcttgtt gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc tccgcaggta
    37921 tcaaaattct gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca
    37981 aaatgggtcg ctatttatgc cgttgatggt cattgcggac aggtacaatt cacgttgtag
    38041 aaatggctgg gggcgaaaat gctgacaata aaacgtagcg tggcgatcat cgcgatactc
    38101 ttcagtccgt taagtacggc gagcaatctc acatcgcaat tgcacaactt ttttagcgcc
    38161 caactcgcgg gggtaagtga tgaggttcgt gtttctattc gtacagcgcc caatctgcta
    38221 ccgccgtgcg agcagccatt gctttcgatg agcaataatt cccgcctgtg gggcaatgtg
    38281 aatgtgttgg cacgctgcgg taacgacaaa cgatatttac aggttaatgt acaggccaca
    38341 ggaaattatg tggttgccgc gatgcccatt gtgcgtggag gaaagctgga agctggcaat
    38401 gtcaaactga aacgcggacg gctggatacc ttgccaccgc gtacggtgct ggatatcaat
    38461 caacttgttg atgccgttag cctgcgcgat ctatcacccg atcaacctat ccagttaacc
    38521 cactttcgcc aggcatggcg ggtaaaagcg ggacaacgcg tcaacgttat cgccagcggt
    38581 gatgggttta gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt cgcacagaat
    38641 gcgcgggtgc gcatgatatc gggacaagtg gtcagcggcg ttgttgatgc agatgggaat
    38701 attcttataa acctgtaagc tgttaaagat tacccgtccc ttgccgataa ataagcaaca
    38761 catgataaaa gcgccctcaa tgaggaataa accatgagta ttgatcgcac ttcgcctctg
    38821 aagccagtaa gcaccgttca accgcgcgaa accactgacg cgccggtaac aaacacccga
    38881 gcggcaaaaa caacagcctc caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa
    38941 ctgatgcaac ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt aaagctggcg
    39001 attcgtaacg gtgaactaaa aatggacact ggcaaaattg ccgatgcgct gatcaacgaa
    39061 gcgcagcaag acttgcagag taattgatcg tatgacgcgt cttgcagaga tcctcgacca
    39121 gatgtccgct gtgcttaacg atctcaaaac ggtaatggat caagagcagc aacatctctc
    39181 tatggggcag atcaacggca gccagttgca atggattaca gaacaaaaaa gctcactgct
    39241 ggcgacgctg gattacctcg aacagttacg caggaaagaa cccaatacag caaatagcgt
    39301 tgatattagt caacgctggc aggaaattgc tgtgaaaacg cagcaactac gccaaatgaa
    39361 tcaacataac ggctggttac tggaaggaca gattgagcgc aatcaacagg cgctggaaat
    39421 gttgaaaccg catcaggaac cgacgctgta tggggcgaac ggtcagacct caacccccta
    39481 tcgcggcagt aaaaagattt cgatctgaag attatctccg gcctgcactg caggccggaa
    39541 tgtatcgtta caccgtccgg cgggcaaatt ctttaacttt gaagcccagt actgccagtg
    39601 cagcgaagta cgcggcaatc cccgccagca cgaccgccat taaacgcagt aaacgccagg
    39661 gcatggtacc caatgaccac tccggcatga tatgtaacat acctaaaagc acgccagaca
    39721 tcaccagtac cgccaccacc agacgcaaca gaaacgccat ccagccgggt tgcggagtaa
    39781 agattttctg cttacgcaac tgccagtaaa gcagcgaagc attcagacac gccgccagac
    39841 caatagaaag tgacagcccg gcatgtttca acggaccaat aaacgccagg ttcatcaatt
    39901 gcgttaaaat cagcgtaacg atggcaattt tcactggcgt tttaatgtcc tggcgggaat
    39961 aaaagccagg agccaacact ttcactacaa tcaggccaat caaacccacc gagtaggcaa
    40021 ttaacgcccg ctgggtcatc agcgcatcaa aggcggtaaa tttaccgtac tggaacagcg
    40081 aaacggttaa cggaccggaa agaatgccca acgcaaccgc acttggcagc gccaacagga
    40141 aacaaagacg cagcccccag tccatcaaac ggttgtattc atcatgattg ccactggcaa
    40201 aacttttcga cagcgacggt agcaaaatgg tgccaagcgc cacgcccagc acaccggacg
    40261 gaaactccat taagcggtcg gcgtaataca tccacgacac cgaaccggaa gcaagaaacg
    40321 aggcaaaaat ggtgttgatg attaaggaga tctggctcac agagacgcca aggattgccg
    40381 gtcccatctg tttcaccaca cgcattgctc cggcatcgtg gaagttaatg cgtggcaaaa
    40441 ccagcatgcc gatcttcttc agatgaggta gctgatacac cagctgcagg atgccgccga
    40501 ccgtaacagc ccaggccagc gccagcaccg gtggattaaa gtacggcgcg gcaaacagcg
    40561 caaaaccaat catgctgata ttgagcaatg tcggggcaaa cgccggaatt gagaagcggt
    40621 tccaggtatt cagaatcgct cccaccagcg acgccaggga gatcagcaag atatagggaa
    40681 aggttatctt tagtagctgg ctggtcaggg caaatttgtc ggcagtgtca gcgaagcctg
    40741 gcgcggtcac catgatcacc cacggtgcag cgagcatgcc agcgaccgtc acaaccgcca
    40801 gcgcaagtgt cagcaggcca gaaacataag agacaaagac ccgcgtggcg tcttcaccct
    40861 gcttgctttt atattctgcc agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa
    40921 agatacggcg taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc
    40981 ctgcgccaaa gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca
    41041 tggtcatcga gctgacggcg gccagcgatt ttaataaatt catcggtgtt ctaatccaga
    41101 cccacgggca aaacgcctgc aaggcaggcg ctgaaagagg cgaatagtct acctggatta
    41161 tggtgaattg ctaccgccag atgttacagg gtttattcac tcatcgcatc gcgccagatc
    41221 ttgtcaacga tacgttgcgc cagcacggcc tgttcgccgg ctgtttgcgg aactgtctga
    41281 ttttgcacgc attcaatgaa gtgccgcgca cagtcgacaa atccacgttg ctcaagcgta
    41341 ctctgccaac caggaatcgg tttatgcact acgccctgcc cgcgctcctc acgccattcg
    41401 cgcatatccg taatgtcgat cagcgcacca tcagtcacgg cctgcacggt ttcacgctga
    41461 cttccggccc ggcgatgcat acaggtggtg atctgcaaag gaccagccga aaaatggtgc
    41521 tcggcaaaca gcatttcgcc ggactcattg gtcagcaacg taccgctttc gagagttgca
    41581 ttgccgccag acaaccacag cgcggtatca accacatgca aataatcatc cagcaatgtg
    41641 aaataaagat cgtgtggccc gacgctattg gtacgatgtt tatccattct tagcgaggac
    41701 gcggtggcga gttgcgtttt taactcaccg tagagtggtg cgaaacgacg gttaaaaccg
    41761 accatcaacg tcagtttttt ccgcgccgcc agttccacca gccgttcagc atcgcgcaga
    41821 ttttctgcca gcggtttatc gacacagaca tgtacccccg cattgagtaa cgtactgacc
    41881 acgccaaagt ggctggcggt gctggaatgc acaaaaaccg catcgcaact ggcggcaagg
    41941 ctggataacg aatcagcata aggaatgcgc cagctgtcac aaattggcag ggctttggcg
    42001 cgcgtaggcg accaggcacc ttgtaacgtc cagtcagacg ctgccgccag caccggtaac
    42061 cacgcttttt gcgcgatgcc gcctaacccc actacgccga tacgtaattt tttcacttta
    42121 atctcccaga tgggccagca acgaatcaag acgctgtttc agttctgcca cttcgatttc
    42181 cagggcttcg acgcgggcct gtaaatcacc gtcaaccgcg ttcgacatat ccgttaccgc
    42241 cggcggattc tcaacctcac cactgaaaag atgcatgtag cggctttcgc gtttacccgg
    42301 ttcgcgggcc agacgcacca caaaaggacc atcttcgcga ttcgccagtt gttccagcgt
    42361 cgactccact tccgccatat cgctgaattc atacattcgc gcggcgcggc tgcgcagttc
    42421 acccggcgtc tgggcaccac gcaataacag cgtggtgatt aacgccactt ctgcggcgct
    42481 cagtttcaga tcgccaaatt ctgaattaca aaaacgttgc tcatatttgg tgacccgatt
    42541 accaaaaccg ctcaccgtgc gtagataatg acgtttgacc agattatcca gctgttcctg
    42601 cacttcagat tcactcagat tcataaccgg ttcacggttc gttttctgat tacaggccgt
    42661 gactacacca tttacggaga gcgggtattg ctccggcgtg gtcacctgtt tttccagcaa
    42721 gcaaccaatc acgcgggctt ccagggcggt cagttgatat ttcatgagat tctccttagc
    42781 ggccgggcgt ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat
    42841 caatcaacag atagtctttc gcatagcctt ctttttcaaa acccagtcgc gccagtaaat
    42901 caccgctgcg tttattgtgc ggcatataat tagccataat gcgatgaata tgttgggtgc
    42961 gctgcatata acgaatggct gcgctcagag cttcaaacat gagtcctttg ccctgccatt
    43021 tttgcccaat cgaataaccg agatagcagg catgaaaaga gccgcgaaca acattggaaa
    43081 aattggcaac gccaataatc tctttttcgt ccgggtcgaa taagccaaag tagaaagctg
    43141 aaccttgttt atgaaattcg ttaatcatcc ctagcctggc ctgccagcct gatggataac
    43201 agtggctttc gtcgcgcact ggctcccagg gcttgaggaa atggcgattc tctgcgtaat
    43261 aatccgccag acgccaggca tcacgatcat gcaccagacg cacgaccagt cggtctgtgg
    43321 ttaagcgcac ttttggcacg ttactgcgat agccaaacat tctataccta ctccttcccg
    43381 taacgtcttt aatacgctgt aagtattact atacctgcgc aaccagaccc tgtgaaaagt
    43441 ccagaatgcc attttctgca ttattcacat atttgataaa aactctctat caaagcgacg
    43501 ttttgataaa aaaatattgt ccccgaagtg atgggaaaag tcttagcggc aatgcagaat
    43561 agaaagtgct catcttttat ttcccgggag gggaaatgtc ccgcgtgtcg caggcgagga
    43621 acctgggtaa atatttcctg ctcatcgata atatgctggt cgtgctgggg ttctttgttg
    43681 tcttcccgct gatctctatc cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg
    43741 gtattgccct cggtctacgc cagtttattc agcaaggtct gggtattttc ggcggcgcaa
    43801 ttgccgaccg ctttggtgcc aaaccgatga ttgtcaccgg tatgctgatg cgtgccgccg
    43861 gattcgccac aatgggtatc gcccacgaac cgtggctact gtggttttca tgcctgcttt
    43921 cgggactcgg cggcacgttg tttgatcctc cgcgttcggc gctggtggtg aaactaatcc
    43981 gtccacaaca gcgtggtcgt tttttctcgc tgttgatgat gcaggacagt gccggtgcgg
    44041 tcattggcgc attgttgggg agctggctgt tgcaatacga ctttcgcctg gtctgcgcca
    44101 caggggcagt tttgtttgtg ctgtgtgcgg cgttcaatgc gtggttgtta ccagcatgga
    44161 aactctccac cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg cgcgacaagc
    44221 gttttgtcac ctatgtcctg acgctggcgg gttactacat gctggctgta caagtgatgc
    44281 tgatgctgcc aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt
    44341 atgccattga agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa
    44401 agcattttcg tctggaacac cggttaatgg ctgggctgtt gataatgtca ttaagcatga
    44461 tgccagtggg catggtcagc ggcctgcaac aacttttcac cctgatttgt ctgttttata
    44521 tcgggtcgat cattgccgag cctgcgcgtg aaaccttaag tgcttcgctg gcagacgcaa
    44581 gagctcgcgg cagctatatg ggatttagcc gtctgggtct ggcgattggc ggcgctattg
    44641 gttatatcgg tggcggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc
    44701 cgtggatgat gctgggcatt attggcatct tcactttcct tgcgctgggt tggcagttta
    44761 gccagaaacg cgccgcgcgt cgtttgcttg aacgcgacgc ctgatttgat actcccccct
    44821 gactcctcca tactgaaaga gaactgacag ccaaatggag gagtcacgtg aagctataca
    44881 tttacgatca ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc
    44941 ccgtcgaatt acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc
    45001 aaaaacaggt tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggaca
    45061 tcgttcacta tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg
    45121 ccattgaaga gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt
    45181 ttgccaaatc ggcatttgat gagttttcta ctcctgccgc gcgcaaatat ttcgtcgaca
    45241 agaaagaggc cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta
    45301 agaatatcag cgatgattta cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga
    45361 atggcgaact ttcggaagat gatattcagc tatttccgct cctgcgtaat ctgacgctag
    45421 tagccggaat caactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga
    45481 cacaaatcaa tttgttatca tcaatggcga tttaatcctg acccgtcgcg gcagcctgta
    45541 ttcgcgggtt gccgccgttt tcagtttttt ctgctggcgc gttatcgctg gtgacacatg
    45601 ctgcgttgtg tcaggatatt gacgcagatt tcatgtcgag gactaccatg aacaagtttt
    45661 tatttgccgc tgcattgatc gtcagtggcc tgctcgttgg ctgtaatcaa ctcacccaat
    45721 acaccatcac cgaacaagaa attaaccagt cgcttgcgaa acataataat ttctcaaaag
    45781 atatcggttt acccggcgtg gctgacgccc atatcgttct gacaaacctg accagccaaa
    45841 ttggtcgcga agagccgaat aaggttaccc taaccggaga cgccaatctg gacatgaact
    45901 ccctgttcgg tagccagaaa gcgaccatga aactgaagct gaaagcgctg cccgtgtttg
    45961 ataaagaaaa aggcgcgatc ttcctgaaag agatggaagt ggtcgatgcg acggtacaac
    46021 cggaaaaaat gcaaacggtg atgcaaacgt tgcttcccta tttaaaccag gcattacgca
    46081 attactttaa ccagcaacct gcttacgtcc tgcgcgaaga tggcagccag ggcgaagcaa
    46141 tggcgaagaa actggcgaaa ggcattgaag tgaagccagg cgaaattgtc attccgttta
    46201 ctgattaatc acgagggcgc attcgcgccc tttatttttc gtgcaaagga aaacgtttcc
    46261 gcttatcctt tgtgtccggc aaaaacatcc cttcagccgg agcatagaga ttaatgactg
    46321 caccatccca ggttttaaag atccgccgcc cagacgactg gcaccttcac ctccgcgatg
    46381 gcgacatgtt aaaaactgtc gtgccgtata ccagcgaaat ttatggacgg gctatcgtaa
    46441 tgcccaatct ggctccgccc gtgaccaccg tagaggctgc cgtggcgtat cgccagcgta
    46501 ttcttgacgc cgtacctgcc gggcacgatt tcaccccgct gatgacctgt tatttaacag
    46561 attcgctgga tcctaatgag ctggagcgcg gatttaacga aggcgtgttc accgctgcaa
    46621 aactttatcc ggcaaatgca accactaact ccagccacgg cgttacgtca gttgacgcaa
    46681 tcatgccggt acttgagcgc atggaaaaaa tcggtatgcc gctactggtg catggtgaag
    46741 taacacatgc agatatcgac atttttgatc gtgaagcgcg ctttatagaa agcgtgatgg
    46801 aaccgctgcg ccagcgcctg accgcgctga aagtcgtttt tgagcatatc accaccaaag
    46861 acgctgccga ctatgtccgt gacggaaatg aacggctggc tgccaccatc actccgcagc
    46921 atctgatgtt taaccgcaac catatgctgg ttggtggtgt gcgtccgcac ctgtattgtc
    46981 tacccatcct caaacgcaat atccaccaac aggcattgcg tgaactggtc gccagcggtt
    47041 ttaatcgtgt attcctcgga acggattctg cgccacatgc acgtcatcgc aaagagagca
    47101 gctgcggctg cgcgggctgt ttcaacgccc caaccgcgct gggcagttac gctaccgtct
    47161 ttgaagagat gaatgctttg cagcactttg aagcattctg ttctgtaaac ggcccgcagt
    47221 tctatggctt gccggtcaac gacacattca tcgaactggt acgtgaagag caacaggttg
    47281 ctgaaagcat cgcactgact gatgacacgc tggtgccatt cctcgccgga gaaacggtac
    47341 gctggtccgt taaataataa aaatcgtagc ttcctgttgt cattaggtta ttttacctgt
    47401 ataaataacc agtatattca acagggggct attatgcgaa ttgaagtcac catagcgaaa
    47461 acttctccat tgccagctgg ggctattgac gccctggctg gcgaactttc ccgccgtatt
    47521 cagtatgcgt ttcctgataa tgaaggccac gtatcggtac gttatgccgc agcaaataat
    47581 ttatcggtta ttggcgcgac aaaagaagat aaacagcgca ttagcgaaat tctccaggag
    47641 acgtgggaaa gcgccgatga ctggtttgtc agcgaataat atgcagtgat ttttgttttt
    47701 gccgggtcgc cccggctttt ttattttact tgcgaattgt gagtacaaaa tttctacact
    47761 tactcaaaat aattgcacaa tatgctaaaa aaatatgaat gacatgctgt tttattgttc
    47821 atataaatct taaaagcaga aatttatctg ttcccccttt aaaaattcgg tgaataccct
    47881 tacttattgg tatactgaag ttgcttcaga taaaaacgca ttgaacctcg aataacgttg
    47941 tctagtaaca cgaattaggg ggccatgatg gaaaaaaata atgaagtcat tcagactcat
    48001 ccgctcgtag ggtgggacat cagcaccgtt gatagctatg atgcgctgat gttgcgtttg
    48061 cactaccaga ccccaaataa gtccgagcag gaagggactg aagttggtca gacgctctgg
    48121 ttaaccactg atgttgccag acaatttatt tcgatattag aagcaggaat cgccaaaatt
    48181 gaatccggtg atttccaggt aaacgagtat cggcgtcatt aacatgacat gaccatccac
    48241 ccaaaaggca cctcaccggt gcctttacca tttctgggtt gtatatctgc attcagttaa
    48301 ttactctgct taaaccatga gagcagagag aaaaaaatga aatacgatct catcattatt
    48361 ggcagcggtt ccgtaggcgc tgccgccggg tattatgcaa cccgcgccgg tttaaacgtg
    48421 ctaatgaccg acgcccatat gccaccgcat caacacggca gccaccacgg cgatacgcga
    48481 ttaattcgcc atgcttatgg tgaaggcgaa aagtatgtcc cgctggtcct ccacgcgcaa
    48541 atgctgtggg atgaactttc ccgccacaac gaagatgatc ccatttttgt acgctctggt
    48601 gtcattaacc tcggtccggc tgactccgca tttctcgcca acgtcgccca cagcgccgac
    48661 cagtggcaac tcaacgttga aaagctcgac gcgcaaggga ttatggcccg ctggccagaa
    48721 atacgcgtcc cggacaacta catcggttta tttgaaactg attccggttt tttgcgcagc
    48781 gaactggcga ttaaaacctg gatccaactg gcgaaggaag cgggctgtgc gcagctgttc
    48841 aactgcccgg tcaccgcaat tcgtcatgac gatgatggcg taactattga aacggcagac
    48901 ggtgagtatc aggcgaaaaa agcgattgtc tgcgcgggaa catgggtaaa agacctactc
    48961 ccggagctgc ctgtccagcc cgtacgcaaa gtatttgcct ggtatcaggc cgatggccgc
    49021 tatagcgtga agaataaatt cccggcattt accggtgagc tgcccaatgg cgatcaatat
    49081 tatggttttc cggcagaaaa cgacgcgttg aagattggca aacataacgg aggccaggtt
    49141 atccattcag cggatgaacg tgttccgttt gcggaagtgg ccagcgatgg ttcggaagcc
    49201 ttcccgttct tgcgcaatgt gttgccgggt atcggttgct gcctgtacgg cgctgcctgc
    49261 acctatgata attcgcctga cgaagatttt attatcgata ccctacccgg ccacgataat
    49321 acactgctca ttaccggcct gagtgggcac ggttttaaat ttgcgtcagt tttaggggaa
    49381 atagctgccg attttgcgca agacaaaaaa agcgattttg atttgacgcc attcaggctt
    49441 tcccgcttcc aataatcatc attacggcct ccagaatgag gccgttttta caggagtatt
    49501 taatgcgtcg actgttgcac tatctcatca ataatattcg cgagcatctg atgctttatc
    49561 tttttctctg gggattgttg gcaattatgg atctgatcta tgtgttttat ttttagtttt
    49621 ccgcactgac ataaactgac aaatatttaa tctccctttc atttagcgtg aaaaattgaa
    49681 tgagattctt cctgcctcta cagca
//
LOCUS       DACADF010000014        48705 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.014, whole genome shotgun sequence.
ACCESSION   DACADF010000014 DACADF010000000
VERSION     DACADF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.014"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(137..841)
                     /locus_tag="ISB30_000809"
     CDS             complement(137..841)
                     /locus_tag="ISB30_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP1988478.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(978..1430)
                     /gene="moaE"
                     /locus_tag="ISB30_000810"
     CDS             complement(978..1430)
                     /gene="moaE"
                     /locus_tag="ISB30_000810"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAP1988479.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1432..1677)
                     /gene="moaD"
                     /locus_tag="ISB30_000811"
     CDS             complement(1432..1677)
                     /gene="moaD"
                     /locus_tag="ISB30_000811"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAP1988480.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(1670..2155)
                     /gene="moaC"
                     /locus_tag="ISB30_000812"
     CDS             complement(1670..2155)
                     /gene="moaC"
                     /locus_tag="ISB30_000812"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAP1988481.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2158..2670)
                     /gene="moaB"
                     /locus_tag="ISB30_000813"
     CDS             complement(2158..2670)
                     /gene="moaB"
                     /locus_tag="ISB30_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAP1988482.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2692..3681)
                     /gene="moaA"
                     /locus_tag="ISB30_000814"
     CDS             complement(2692..3681)
                     /gene="moaA"
                     /locus_tag="ISB30_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAP1988483.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLENDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3669..3813)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4078..4986
                     /gene="yvcK"
                     /locus_tag="ISB30_000815"
     CDS             4078..4986
                     /gene="yvcK"
                     /locus_tag="ISB30_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAP1988484.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTAP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5024..7045)
                     /gene="uvrB"
                     /locus_tag="ISB30_000816"
     CDS             complement(5024..7045)
                     /gene="uvrB"
                     /locus_tag="ISB30_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAP1988485.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7624..8301)
                     /gene="bioD"
                     /locus_tag="ISB30_000817"
     CDS             complement(7624..8301)
                     /gene="bioD"
                     /locus_tag="ISB30_000817"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAP1988486.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIEALVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(8294..9049)
                     /gene="bioC"
                     /locus_tag="ISB30_000818"
     CDS             complement(8294..9049)
                     /gene="bioC"
                     /locus_tag="ISB30_000818"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAP1988487.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     RVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9036..10190)
                     /gene="bioF"
                     /locus_tag="ISB30_000819"
     CDS             complement(9036..10190)
                     /gene="bioF"
                     /locus_tag="ISB30_000819"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAP1988488.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVVDDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSWSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10187..11227)
                     /gene="bioB"
                     /locus_tag="ISB30_000820"
     CDS             complement(10187..11227)
                     /gene="bioB"
                     /locus_tag="ISB30_000820"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAP1988489.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11314..12603
                     /gene="bioA"
                     /locus_tag="ISB30_000821"
     CDS             11314..12603
                     /gene="bioA"
                     /locus_tag="ISB30_000821"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAP1988490.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETSRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12662..13138
                     /locus_tag="ISB30_000822"
     CDS             12662..13138
                     /locus_tag="ISB30_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAP1988491.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13290..14573
                     /locus_tag="ISB30_000823"
     CDS             13290..14573
                     /locus_tag="ISB30_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAP1988492.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(14714..16975)
                     /locus_tag="ISB30_000824"
     CDS             complement(14714..16975)
                     /locus_tag="ISB30_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAP1988493.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSEMAEVFARIKQIAGQEHVDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(17158..18591)
                     /locus_tag="ISB30_000825"
     CDS             complement(17158..18591)
                     /locus_tag="ISB30_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAP1988494.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(18667..19719)
                     /locus_tag="ISB30_000826"
     CDS             complement(18667..19719)
                     /locus_tag="ISB30_000826"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAP1988495.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            19903..20856
                     /locus_tag="ISB30_000827"
     CDS             19903..20856
                     /locus_tag="ISB30_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1988496.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFSIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(20897..21892)
                     /gene="pgl"
                     /locus_tag="ISB30_000828"
     CDS             complement(20897..21892)
                     /gene="pgl"
                     /locus_tag="ISB30_000828"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAP1988497.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            22047..22865
                     /locus_tag="ISB30_000829"
     CDS             22047..22865
                     /locus_tag="ISB30_000829"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HAP1988498.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(22866..23924)
                     /gene="modC"
                     /locus_tag="ISB30_000830"
     CDS             complement(22866..23924)
                     /gene="modC"
                     /locus_tag="ISB30_000830"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAP1988499.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGCKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(23927..24616)
                     /gene="modB"
                     /locus_tag="ISB30_000831"
     CDS             complement(23927..24616)
                     /gene="modB"
                     /locus_tag="ISB30_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAP1988500.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(24616..25389)
                     /gene="modA"
                     /locus_tag="ISB30_000832"
     CDS             complement(24616..25389)
                     /gene="modA"
                     /locus_tag="ISB30_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1988501.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(25556..25705)
                     /locus_tag="ISB30_000833"
     CDS             complement(25556..25705)
                     /locus_tag="ISB30_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAP1988502.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            25834..26622
                     /gene="modE"
                     /locus_tag="ISB30_000834"
     CDS             25834..26622
                     /gene="modE"
                     /locus_tag="ISB30_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAP1988503.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            26690..28162
                     /gene="modF"
                     /locus_tag="ISB30_000835"
     CDS             26690..28162
                     /gene="modF"
                     /locus_tag="ISB30_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAP1988504.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTSRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQHLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSELLEGVQMPEPDEPSARHALPANEPRIVLNNGVVSYNERPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIN"
     gene            28425..29441
                     /gene="galE"
                     /locus_tag="ISB30_000836"
     CDS             28425..29441
                     /gene="galE"
                     /locus_tag="ISB30_000836"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP1988505.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRRAGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            29451..30497
                     /gene="galT"
                     /locus_tag="ISB30_000837"
     CDS             29451..30497
                     /gene="galT"
                     /locus_tag="ISB30_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAP1988506.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            30501..31649
                     /gene="galK"
                     /locus_tag="ISB30_000838"
     CDS             30501..31649
                     /gene="galK"
                     /locus_tag="ISB30_000838"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP1988507.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            31643..32683
                     /gene="galM"
                     /locus_tag="ISB30_000839"
     CDS             31643..32683
                     /gene="galM"
                     /locus_tag="ISB30_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAP1988508.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            32886..33638
                     /gene="gpmA"
                     /locus_tag="ISB30_000840"
     CDS             32886..33638
                     /gene="gpmA"
                     /locus_tag="ISB30_000840"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP1988509.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(33805..34857)
                     /gene="aroG"
                     /locus_tag="ISB30_000841"
     CDS             complement(33805..34857)
                     /gene="aroG"
                     /locus_tag="ISB30_000841"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAP1988510.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            35173..35553
                     /locus_tag="ISB30_000842"
     CDS             35173..35553
                     /locus_tag="ISB30_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988511.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            35667..36608
                     /gene="zitB"
                     /locus_tag="ISB30_000843"
     CDS             35667..36608
                     /gene="zitB"
                     /locus_tag="ISB30_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAP1988512.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRLCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNRGVSGHSHHHH"
     gene            complement(36605..37324)
                     /locus_tag="ISB30_000844"
     CDS             complement(36605..37324)
                     /locus_tag="ISB30_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAP1988513.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWIKSARERGSRALSH"
     gene            complement(37362..38405)
                     /gene="nadA"
                     /locus_tag="ISB30_000845"
     CDS             complement(37362..38405)
                     /gene="nadA"
                     /locus_tag="ISB30_000845"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAP1988514.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKANYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLQHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(38683..38758)
                     /locus_tag="ISB30_000846"
     tRNA            complement(38683..38758)
                     /locus_tag="ISB30_000846"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38723..38725),aa:Lys,seq:ttt)
     gene            complement(38792..38867)
                     /locus_tag="ISB30_000847"
     tRNA            complement(38792..38867)
                     /locus_tag="ISB30_000847"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38832..38834),aa:Lys,seq:ttt)
     gene            complement(38916..38991)
                     /locus_tag="ISB30_000848"
     tRNA            complement(38916..38991)
                     /locus_tag="ISB30_000848"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38956..38958),aa:Lys,seq:ttt)
     gene            complement(38995..39070)
                     /locus_tag="ISB30_000849"
     tRNA            complement(38995..39070)
                     /locus_tag="ISB30_000849"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39035..39037),aa:Val,seq:tac)
     gene            complement(39122..39197)
                     /locus_tag="ISB30_000850"
     tRNA            complement(39122..39197)
                     /locus_tag="ISB30_000850"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39162..39164),aa:Lys,seq:ttt)
     gene            complement(39200..39275)
                     /locus_tag="ISB30_000851"
     tRNA            complement(39200..39275)
                     /locus_tag="ISB30_000851"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39240..39242),aa:Val,seq:tac)
     gene            complement(39311..39386)
                     /locus_tag="ISB30_000852"
     tRNA            complement(39311..39386)
                     /locus_tag="ISB30_000852"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39351..39353),aa:Lys,seq:ttt)
     gene            complement(39551..40342)
                     /gene="cpoB"
                     /locus_tag="ISB30_000853"
     CDS             complement(39551..40342)
                     /gene="cpoB"
                     /locus_tag="ISB30_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAP1988515.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVVASTTPTADAGTSNAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(40352..40873)
                     /gene="pal"
                     /locus_tag="ISB30_000854"
     CDS             complement(40352..40873)
                     /gene="pal"
                     /locus_tag="ISB30_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAP1988516.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            complement(40908..42200)
                     /gene="tolB"
                     /locus_tag="ISB30_000855"
     CDS             complement(40908..42200)
                     /gene="tolB"
                     /locus_tag="ISB30_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAP1988517.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(42333..43643)
                     /gene="tolA"
                     /locus_tag="ISB30_000856"
     CDS             complement(42333..43643)
                     /gene="tolA"
                     /locus_tag="ISB30_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAP1988518.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAAD
                     KKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNA
                     SPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLD
                     IKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(43708..44136)
                     /gene="tolR"
                     /locus_tag="ISB30_000857"
     CDS             complement(43708..44136)
                     /gene="tolR"
                     /locus_tag="ISB30_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAP1988519.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(44140..44832)
                     /gene="tolQ"
                     /locus_tag="ISB30_000858"
     CDS             complement(44140..44832)
                     /gene="tolQ"
                     /locus_tag="ISB30_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAP1988520.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(44829..45233)
                     /gene="ybgC"
                     /locus_tag="ISB30_000859"
     CDS             complement(44829..45233)
                     /gene="ybgC"
                     /locus_tag="ISB30_000859"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAP1988521.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(45383..45676)
                     /gene="ybgE"
                     /locus_tag="ISB30_000860"
     CDS             complement(45383..45676)
                     /gene="ybgE"
                     /locus_tag="ISB30_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAP1988522.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGFFCPLLADIVLIVGLIFFFF"
     gene            complement(45676..45789)
                     /gene="cydX"
                     /locus_tag="ISB30_000861"
     CDS             complement(45676..45789)
                     /gene="cydX"
                     /locus_tag="ISB30_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAP1988523.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(45804..46943)
                     /gene="cydB"
                     /locus_tag="ISB30_000862"
     CDS             complement(45804..46943)
                     /gene="cydB"
                     /locus_tag="ISB30_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAP1988524.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(46959..48527)
                     /gene="cydA"
                     /locus_tag="ISB30_000863"
     CDS             complement(46959..48527)
                     /gene="cydA"
                     /locus_tag="ISB30_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAP1988525.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        11951 a        13010 c        12068 g        11676 t
ORIGIN      
        1 acttttgcat ttatggatac agaaaacgct tccgctcgtc cataaggcat attttatacc
       61 tggtacttgt taagtgtcgt ttttaataag aaaaaaaatc ccgacacatg gccgggatat
      121 tgatgatgaa aaagaattaa cggcggttgc cgaagatccg cagcaacatc aggaacaggt
      181 tgatgaagtc cagatacaag gttaacgcgc caagaatgga atatttgcgc aggttcgacg
      241 tgtcgcgagt atcaatctgc tcacccatat ttttcagttt ctgggtgtca tacgccgtca
      301 atccgacaaa gacaatcacg ccgatgtagg tgactgccca catcaatgct tcgcttttca
      361 gccagaagtt aaccagcgat gccagcacaa tgccgattaa cgccataaac agcatattgc
      421 caaagccgct tagatcgcgc ttcgtggtgt aaccgtacag gctcattgcg ccgaacatcc
      481 cggcagtaac gacgaaagta ctggcgatag aagcagcggt atagacaatg aaaatactgg
      541 aaagcgtaag acccgtcagc gccgaataaa gcataaagag catcgtcgtt acacctgcgc
      601 tcagcttttg aatcatcgct gataacacaa taaccaatgc taattgcgcg atgatcagac
      661 cgattaaaaa gacacggtta gtaaacaaca gctccatcac ggccgcggaa ttagccgcat
      721 accaggcaac aaatgctgtc agcaacaagc caacggtcat ccagccatag acttgcgcca
      781 tataagtttg caagccagcc cggggttgta cgattgaatc agaacgtggg aatctgtcca
      841 tgactttctc ctgaagatat aaggaatatc ttaaggatac tgcaaaatga tgaggctgtg
      901 catcgacgca gcgtaaacgg atgtactgag cggtgaaatt gccggacgca gcggtgcctt
      961 atccggctaa caataaacta ccagcgtttt gccgcctgct gatcgctctc ccgagcttcg
     1021 acccagcggt cgccttccgg cgtggcttcg cgcttccaga acggtgcgcg ggttttgaga
     1081 taatccataa taaactgccc ggcttcaaac gcactgctgc gatgcgcact ggtaacaccg
     1141 acaaaaacaa tttcatcgcc cggccataat tcaccaatgc ggtgaattac cgtgacgcgc
     1201 cccagcggcc agcggttacg cgcttcatca acgatttctg ccagtgcttt ttctgtcatt
     1261 cccggatagt gttcgagggt taatgctttg acgctgtcgc ccaggttatg gttgcgcacc
     1321 ttaccggtaa aggtgactac cgcaccgtct tcgtcacgct cagccagcca cgggtactct
     1381 tctcctacgc tgaacggctg cgggccgaca acaattttgg tttctgccat cttaacctcc
     1441 ggttaccggc gggaagaaag ccacttcgtc gccgtcagtc agcgaatggt caaaactcac
     1501 cagcgtctgg ttgacagcag ccagcaattt gccatcttcc agcgccagag cccagcgatc
     1561 gctctgcgca gccagatgct ggcgtaacgc ttcaacggtt gggaaatccg cagccacttc
     1621 ggttgcatca gttcccacca actcgcgcac ctgggcgaaa aaaagaacgt taatcatccg
     1681 cttccacctt aaagtcaccc gacttgccgc cgctctttgc cagcaaacgt accggaccaa
     1741 tcaccatatc tttttgcacc gctttgcaca tgtcataaat ggtcagcgcc gccacggagg
     1801 ccgcggttaa cgcttccatt tcgacaccgg ttttcccggt caggcggcat aaggtttcta
     1861 tacgtacccg attgtgctcc ggctcggcct gtaaattgac ttcaactttg ctgagcatca
     1921 gcgggtgaca cagcgggatc agatcccagg tgcgttttgc cgcctgaata ccggcgatac
     1981 gcgcagtggc aaatacgtcg cctttgtggt ggcgaccatc aataatcatc gccagcgtct
     2041 cgctgcgcat ggtgacaaag gcttcggcgc gcgcttcacg cacggtttcc gctttggcgg
     2101 agacatcgac catgtgcgct tcgccagcgg cgttgatatg ggtcagttgc gacatactta
     2161 tttcttcaaa tgtggatgga aattacacgg acgcgtacgg gcatccagct gcggcgcaat
     2221 gatattttcc catgcggtac ggcacgcttt ggtcgaaccc ggcatggcga aaatcagcgt
     2281 tttgttagcg acgcccgcta ccgcacgaga ttgcaacgtg gaagtgccaa tctcttcgaa
     2341 cgacaacatc cggaacactt caccaaaacc ttcaacttca cggtcgaaca acggcagcag
     2401 cgcttcggga gcctgatctc cttccgtcag gccagtacca cccgtaatca acaccacttg
     2461 tacatcgtcg ctggcgatcc acgcagagac ctgagcgcga atagcgtagc ggttttcttt
     2521 cacaatggct ttatcgacaa cgtgatggcc cgcttcttgc gccgagtcgc gaagatagtg
     2581 accggaggta tcgtcttctt caccgcgacg attagaaacc gtaagaatag caatacgggt
     2641 cgggataaat tcagtgctta cctgactcat ctgatctctc cttttgacgt tttagccacc
     2701 aatgtacgat aagttttgcg taataccggt gttattttga tgcaggaaat gggtctgttt
     2761 tttctcccgc agcgccgctg aaatacgcgc ttccagcgcc tgttgctggg tatcgttttc
     2821 cagcagatcg cgcaggttaa cgccgccttc accaaacagg cagagatgga gtttaccaat
     2881 ggaggaaacg cgcaggcggt tgcaagtggc gcagaaatct ttttcatacg gcatgataag
     2941 gccaatctct ccggcgtaat ccggatgaca aaagacttgc gcgggaccgt cgctgcgttg
     3001 acgtaattgg tggatccagc cgcgacgcag taactcgtca cgcagaacct gaccagagat
     3061 gtgatgctta cggaagagct cgctgccctc gcccgtttcc atcagttcga tgaaacgcag
     3121 ctggatggga cgatgctgga tccagttcag aaaggtgtcg agctggtgat gattcacatc
     3181 acgcatcagc acggtattga ctttgacctt ctcaaaaccg gcctcaaatg cagcatcgat
     3241 tcctgccatg acctgattga atttatcctg cccggtaatg gcatgaaact ggcgagcgtc
     3301 aaggctatca acgctgacgt tgatgccagt aagtccagca tcgcgccagt tcgccacatc
     3361 gcgctccaga cggtaaccat tggtggtgac cgctatctgg cgaatagcgt cgttttctcg
     3421 cacagcggcg atgatatcgg taaagtcgcg gcgtaaagat ggctcaccac ctgtcagacg
     3481 gactttttcg gtgcccagac tggcgaaggc gcgcgtaacc cggcgaattt catcgacggt
     3541 caggaagcct ttattggtga cgccgctcgg cttgtaacca tccggcaggc agtaggtgca
     3601 acgaaagtta cacacatcag taatcgacag gcgcaagtag taaaacttac gcgcaaatgc
     3661 atcagtcagt tgtgaagcca tgtacacctt tccagatacg ggaggcgaag tcatttcttc
     3721 cttcgccctg gtggcaatct ttacgagcat tgtcacggcc aaagcaccgt atcagttgac
     3781 ccaggtgcag aggctagagt gtttagtggt taagccgata ctagcgtgta aatgagattt
     3841 ttaccatcca cactttcgct atgtaatcat gtatatagcg tcatgatcgt gctatttcgc
     3901 tacagaataa gggaaaatgg cggttttact ttgatataca tcatcaggga ggcatagcgt
     3961 gcatcgtcct ttagaggtag aagaaaatag tttgtatcct caatattggc aggttaattg
     4021 ctgtttcctc gcaatttgcg ctactgtagc gcggataatc tgactccagg aaactatatg
     4081 cgcaatcgta cgctggctga tcttgatcgt gtcgttgctc tcggcggagg gcatggactg
     4141 ggacgcgttc tctcatcact ttcgtctttg ggttctcgtt taacgggtat cgtcaccacc
     4201 accgataacg gtggctcgac ggggcgtatt cgccgctcag aaggcggcat tgcctggggc
     4261 gatatgcgca actgcctcaa tcagctgata actgaaccca gcgtcgcttc cgcaatgttt
     4321 gaatatcgtt tcggcggtaa tggcgaactt tccgggcaca accttggaaa cctgatgtta
     4381 aaggcactgg atcaccttag cgtgcgacct cttgaagcga tcaatctgat tcgaaatctg
     4441 ctgaaagtgg acgcacattt gattccgatg tcggagcatc ctgttgatct gatggcgatt
     4501 gacgatcagg ggcatgaagt ttacggcgag gtcaatatcg accagttaac tgcgccgatt
     4561 caagagttat tgttaacgcc gaatgtaccc gcaacgcgtg aggcggttca cgctatcagt
     4621 gaagcggatc tcatcattat tgggcctggc agtttttaca ccagcctgat gccaattctg
     4681 ctgctgaagg aaatcgcaca ggcattacgc cgcacgccag cgccgatggt ctatatcggc
     4741 aatctggggc gtgagttgag tttacctgcg gctaatctga agctggaaag caagctggca
     4801 attatggagc agtatgttgg taaaaaggta attgatgcgg tcatcgtcgg gccaaaagtg
     4861 gatgtctcgg cggtgaaaga gcggattgtg attcaggaag tgctggaggc cagcgatatt
     4921 ccgtatcgtc atgaccgcca gttgttgcat agcgcgctgg aaaaggcatt acaggcttta
     4981 ggttaacacg aagccggaag aatattccgg cttcgtcatc ttgtcacgac gcggcaataa
     5041 acagctcacg caactgatgc aactggtcac gaatttgcgc cgcttcttcg aactccagat
     5101 tctgcgcgtg ttgcatcatc aacccttcca gttcatggat cttctgctgt aacgctttag
     5161 gcgacatatc catcggcaca ttatccggct caacaatcgg gcgcgatttt cctctgccct
     5221 tcgctttggt tttggcaatg ttctgcccca gcgccaggat atcgaccact ttcttgttca
     5281 agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctcg cggcgacgtt
     5341 cggtttcgcc aatcgctttc gccattgatg gggtgatctt atcgccgtag agaatcgctt
     5401 taccgttaac gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca
     5461 ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga aacctccggc atatccagac
     5521 cttcgcgcag taagttgatc cctaccaata cgtcgaactc acccagacgc aagtcgcgga
     5581 taatctccat acgttcgacg gtatcgatat ctgagtgaag ataacgcacg cgctcaccgt
     5641 gttcttcgag atattcagtg agatcttccg ccatccgctt ggtcagagtt gtaaccagta
     5701 cgcgttcgtt aattgccgct cgctgacgaa tctccgatag aagatcatcc acctgtgtcg
     5761 ctaccggacg cacttcaata atcgggtcga gtaagcctgt tggacgcaca acctgatcca
     5821 ccacatcgcc accggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg
     5881 tttgcggcgc taatgcttcg aactcttcaa atttcagcgg gcggttatcc agcgctgatg
     5941 gcaagcggaa gccgtactcc accagtgtct ctttacgcgc ccggtcaccg cgatacatgc
     6001 cgccaatttg tggaatggtg acgtgagatt catcgaccac cagcagccca tcagcaggca
     6061 ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg accggagagg aagcgcgagt
     6121 agttctcaat ccccgaacag tagcctagct cgttcatcat ctccagatca aactgggtac
     6181 gctgggtcag ccgctgctct tccagcagtt tgttgttttc caatagcact ttacgtctgg
     6241 cggccagctc ttctttgatc tcctccatcg cctggacgat gcgctcgcgt ggcgtgacgt
     6301 agtgcgtttt cgggtagatg gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg
     6361 ggtcaaataa cgacaatcgt tccacctcct cgtcaaacag ctccacgcga agtgcaatgt
     6421 catccgattc tgccgggaag atatcaatca cctcgccacg aacacggaaa gtaccacgct
     6481 ggaatgcttg atcattacgg gcatattgca gctccgccag tcggcgcagg atcgcgcgct
     6541 gatcgataat cataccgacc gtgagatgga gcatcatctt gagatataaa tcaggatcgc
     6601 ccagaccata aatcgcggaa acagacgcca ccacaaccac atcacgtcgc tccagcatcg
     6661 ctttggtggc ggataaacgc atctgttcga tatgttcgtt taccgaggca tctttctcaa
     6721 taaaggtgtc ggaactcggt acataggcct ccggctgata gtagtcgtag taggagacga
     6781 aatattccac cgcgttttct gggaagaatt ctttcatttc gccatacagc tgggccgcca
     6841 gcgttttgtt gggcgcaagt accatggttg ggcgctgaag gtcagcaatg acattggcaa
     6901 tggtgaaggt tttcccggag ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt
     6961 ccagcccctc ttcgagacgt cgaatcgcct ccggctgatc gccagaaggt ttaaaagcgg
     7021 aattcagttt gaacggttta ctcatgagtc gctacctgaa ggagttgggc gggcaggtat
     7081 gtaattttac tcgtcgtact taattatgcc aacaaattat actggataaa aaaacagttc
     7141 atcaccataa tatttctgtt acagcgtaaa ctccgctgtc aatcatgaac aagatttact
     7201 ctgcggcgag ataaaactcc ggccttaccg ggttatcccc aaagcaacgg cttttttaac
     7261 atttgtcaag atgattgatg acagttttat ggcaagagat gcctgttcag tgactgccat
     7321 tgcaattatc taacctgtta aaaaataaaa gatatttctt tgagctgtct ttaacgccaa
     7381 ttcgcgtgca agccgcgtat tctctcgctt gcctcgtgtt ttctaactct aatacacatg
     7441 gttatccaca ggaatagtgg ataactgtct gcagccccta tcccccgccg cctgggcaat
     7501 tcccacattc accgcatgat gcggaggtaa aatttttttc gtgatttttt tagaatttaa
     7561 ttggttaaat tgcagtcaat cggagacacg atctcgctcg caatttaacc aattacagga
     7621 tgactacatt aaggcaagat tgatgtactt tccggttgcc gcattttctg gattttctgc
     7681 aagccaggga atttcgccca gcagcggcgc gggaatcatg cgggtgagcg tggtcatata
     7741 ttcagcgtga cgttttcccg gaggcgtgac atcgttcgcc acccaccccg ccagagtcag
     7801 tccggcgtgt agtattgcct gcgcagtcaa catcgcgtga ttaatacagc cgagtttcac
     7861 gcccacaacc agtatcactg gcagttgttc ctgtgttacc caatccgcaa aagtgaaagt
     7921 gtcagaaagc ggcgtaaacc agccgccagc accttccact aacacccagt cagcctgttg
     7981 ttcaagcgcg cgtaatccgg cgctcattac caatgcttct atcggcctgc cctcttgcgc
     8041 gctgatgata tgcggcgagg tgggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc
     8101 cagctgcagg ctactgttgc gctgtaacgc cagcgcgtca ctattacgta agcctttcgg
     8161 ggtcttttcg ctgccagagg ccaccggttt gtaacctgcc gtccggtagc ctgctgcctt
     8221 tgcggcttgt aaaagcgcgc agctggcgac agttttcccc acttcggtat ccgttccggt
     8281 gacaaaataa catttactca cgagcaatca ctcccaaaaa aagatgatac gtcagaggat
     8341 atcgcccctg ttgttgcggc caggccagtt gcaatcgctg caactgcgaa cgcgttaata
     8401 ttcgcgggtc acgcccttca tgaagatgcg tggcaccgat gcctttcagc gaacgcatgg
     8461 cactgagcgc atcatcaaac cacagcgtga tgggctgaat ataatgttga taatgcacgg
     8521 cgtttagcga ctgttcgatt tcatctggcg gtaaaaagcg attagcatgc ggacgctcgt
     8581 ccaccgcctg ccacgcctga tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg
     8641 cgaccacgcc tccggggcgc accacccgat acagctcgcg gagtgccgtg gataaattac
     8701 cgcaccactg caccgcgaga ttgctccatg caagatcgaa cgtcgcagtc gctaacggca
     8761 gggattcgat atctcccgcc agataatggt ctgcggcatc cttctggcgt gcctgaacaa
     8821 gcattggcgg agagagatct aacgcagtca cctgcgcgtg acgctctcgc cagcggcggc
     8881 tcatccagcc tggtccacaa cccgcatcca gtacgcgggt gtatttacgc tgtgggagca
     8941 ttgccagtaa ggcgtcagca ctctggcgct gtagatcggc gtgttgctca tagtgtgcgg
     9001 ctgcccgtcc aaatgccgca gcaatggctt gtttattaac cgttgccatg cagcacctcc
     9061 agcagacggt cgatatcctg catttcatgc gctgcggtta gcgttaagcg cagtcgcgca
     9121 gtaccagcgg gtacggttgg cgggcgaatt gccgtgaccc aacagccttg ctggcgcagt
     9181 ttttctgcca gttgtaacgc acggctgtta tcaccgacga tcaatggctg gatggcgctc
     9241 catgaatcag caagcgtaaa cggcaaatcc tgcactccgg cacgaaaacg cgttatgagt
     9301 gccgccagtt tttcgcgccg tgcatccccc tcatcactgc gaatgactgc cagcgacgca
     9361 cgtaatgcct gcgcctgagc gggcggcata ctggtgctgt agataagatg gcgagcgaat
     9421 tgcagcagat aatccgccac cgtattggag caaagcaccg ctgccccgct gacgccaaat
     9481 cctttgccaa acgtcaccac cagcaattct ggttttacct tttgcagcca gcagctgcca
     9541 cgcccctgct ccccgataac gcccgtgccg tgggcgtcat cgaccatcaa ccagccatcg
     9601 tgctgttgcg ttacctgctg gatttcctcc agtggcgcac tatcgccgtc catgctgaac
     9661 acgccttctg tcactaccag ttgctgcccc ggacaggggg aagcaagcag tcgcgccaga
     9721 tgagtgacat cgttatgagc aaaacggcga agcggcgacg ggcttaaact tgcagcctcc
     9781 agcaatgagg catggctaag ccggtcggca acaatacggt cctctttcgc catcatcgcg
     9841 gcaatgactg cctgattagc agcaaaacca gagataaaca gcagtgcccg cgaatagccc
     9901 agccactcgg ccagttcttc ttccagcgcc tgatgcgcca cgctataacc gctgacgtga
     9961 ccggagccgc cgctaccgat gccaaattgc tccgcaccct gcttccaggc acggataatt
    10021 tgcggatgat ggcttaaacc taaataatcg ttactggaaa agttcagata ctggcgatca
    10081 tccaccacca gccagcgtcc ggctccttgc gccaccggat aacggcgacg cagggcatcg
    10141 gcagcacgcc gcgcatcgag cgccgcgttg attttctcct gccagctcat agtgctgccg
    10201 cgttgtaata ttcgtcagtg tccggggtca tcagcgcctg ctccagacgc tgctgttgtt
    10261 cgttatcgcc cgccagcacg gcagtttgct gcggatttag ccccagtttg cggaacagtt
    10321 gcaggtcttt atcttcttcc ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg
    10381 agtttgcgcc agccataaag cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg
    10441 cagaaaggcg tacgtaagag gtcggcatca tgatccgcgc aactgcaatg gtgcgaataa
    10501 aatcaaaggc atcgacatca tcgttatccg ccagcggcgt gcctttcacc ttcaccagca
    10561 tgttgattgg tacgctttcc ggcggcgttg gcaggtttgc cagttgcagc aataatccgg
    10621 cgcgatcttt tactgtttcg cctaagccca caatgccgcc tgaacagact ttgatcccgg
    10681 cgtcgcgcac tttttccagc gtatcgaggc gttcctgata ggtacgcgtg gtgatgatat
    10741 tgccgtaaaa ctccggcgag gtgtcgaggt tgtggttgta gtaatccagc ccggcattcg
    10801 ccaggcgctg cgcctgagat tcactcaacg tgcccagcgt catacacgcc tccagcccca
    10861 tcgcttttac cccctgcacc atttgttcca ggtacggcat atcgcgttcg tggggattct
    10921 tccacgccgc gcccatacag aaacgcgtcg atcccgccgc tttcgctttg cgcgccgact
    10981 ccagcacctg ttcaacttcc atcaaccgct cggcttccag cccggttttg tagcgcgagc
    11041 tttgcgggca atatttgcaa tcttccggac aagctccggt cttaatcgac agcaaagtgc
    11101 tgacctgcac ctgacgagga tcgaaatgct gacgatgcac ctgctgcgct tcaaacagca
    11161 gatccagcaa cggtttttca aataattctg tgacttgcga caatgtccag cgtgggcggt
    11221 gagccatggg gcttctccaa aacgtgtttt ttgttgttaa ttcggtgtag actcgtaaac
    11281 ctaaatcttt tcaatttggt ttacaagtcg attatgacaa cggacgatct tgcctttgac
    11341 caacgccata tctggcaccc atacacatcc atgacctccc cgctgccggt ttatccggtg
    11401 gcaagcgccg aaggttgcga gctaattttg tctgacggca gacgcctggt tgacggtatg
    11461 tcgtcctggt gggcggcgat ccacggttac aatcacccgc agcttaatgc ggcgatgaag
    11521 tcgcaaattg atgctatgtc gcatgtgatg tttggcggta tcacccatgc gcccgccatt
    11581 gagctgtgcc gcaaattggt ggcaatgacg ccgcaaccac tggagtgcgt ttttctcgcg
    11641 gactccggct ccgtagcggt agaagtggcg atgaaaatgg cgttgcagta ctggcaggcc
    11701 aaaggcgaag cgcgccagcg ttttctaacc ttccgcaatg gttatcatgg cgataccttt
    11761 ggcgcgatgt cggtgtgcga tccggacaac tcaatgcaca acctgtggaa aggctatctg
    11821 ccagaaaacc tgtttgctcc cgccccgcaa agccgcatgg atggcgaatg ggatgagcgc
    11881 gatatggtgg gctttgcccg cctgatggcg gcgcatcgtc atgaaatcgc ggcggtgatc
    11941 attgaaccga ttgtccaggg cgcaggcggg atgcgcatgt accatccgga atggttaaaa
    12001 cgaatccgca aaatgtgcga tcgcgaaggt atcttgctga ttgccgacga gatcgccacc
    12061 ggatttggtc gtaccggcaa actgtttgcc tgtgaacatg cagaaatcgc gccggatatt
    12121 ttgtgtctcg gtaaagcctt aaccggcggc acaatgaccc tttccgccac gctcaccacg
    12181 cgcgaggttg cggaaaccat cagtaacggc gaagccggct gctttatgca tgggccaact
    12241 tttatgggca atccgctggc ctgcgcagca gcaaacgcca gcctggcgat tctcgaatct
    12301 ggcgactggc agcatcaggt ggcggctatt gaagcgcagc tacgcgagca acttgccccc
    12361 gcctgtgacg ccgaaatggt tgccgatgtg cgcgtactgg gggcaatcgg cgtggtcgaa
    12421 acctctcgtc cggtgaatat ggcagcgctg caaaaattct ttgtcgaaca gggtgtctgg
    12481 atccggcctt ttggcaaact gatttacctg atgccgccct atattattct cccgcaacag
    12541 ttgcagcgtc tgaccgcagc ggttaatcgt gcggtgcagg atgaaacatt tttttgccaa
    12601 taacgggaag tcagcgtgag ggtttctggc tacactttct gcaaacaaga aaggagggct
    12661 catgaaactc atcagtaatg atttacgcga tggcgataag ctgccgcatc gtcatgtctt
    12721 taacggcatg ggttacgatg gcgataatat ttcaccgcat ctggcgtggg atgatgttcc
    12781 tgtgggaacg aaaagttttg ttgtcacctg ctacgacccg gatgcgccaa ctggctccgg
    12841 ctggtggcac tgggtagttg ttaatttacc cgcggatacc cgcgtattac cgcaagggtt
    12901 tggatctggt ctggtagcaa tgccagacgg cgttttgcag acgcgtaccg actttggtaa
    12961 aaccggatac gatggcgcag cgccgccgaa aggcgaaacc catcgctaca tttttaccgt
    13021 tcacgcgctg gatgtagaac gtattgatgt cgatgaaggt gccagcggcg cgatggtcgg
    13081 gtttaacgtt cattttcact ctctggcgag tgcctcgatt actgcgatgt ttagttaatc
    13141 actctgccag atggcgcaat gccatctggt atcacttaaa ggtattaaaa acaacttttt
    13201 gtctttttac cttcccgttt cgctcaagtt agtataaaaa agcaggcttc aacggattca
    13261 tttttctatt tcatagcccg gagcaacctg tgaacacatt ttcagtttcc cgtctggcgc
    13321 tggcattggc ttttggcgtg acgctgaccg cctgtagctc aacaccgccc gatcaacgtc
    13381 cttctgatca aaccgcgcct ggtacctctt ctcgcccgat tctgtcggca aaagaagcgc
    13441 agaatttcga tgctcaacac tattttgcat ccctgacacc aggtgcggca gcgtggaatc
    13501 cttccccgat taccctgcct gcgcaacctg actttgttgt cggcccggcg ggtactcaag
    13561 gtgtaacgca taccacgatt caggcggcgg tagatgcggc aattatcaag cgtaccaaca
    13621 agcgccagta tattgccgtg atgcctggtg agtatcaggg aacggtgtat gtccctgccg
    13681 ctccgggtgg aattactctg tacggtacag gtgaaaaacc gattgatgtg aagattgggc
    13741 tttcccttga tggtggcatg agccctgccg actggcgtca cgacgtcaac ccgcgcggca
    13801 aatatatgcc aggtaaaccg gcgtggtata tgtacgatag ctgccagagt aaacgcagcg
    13861 acagtatcgg tgttctctgc tctgcggtct tctggtcaca aaacaatggc ctgcaactgc
    13921 aaaacctgac catcgaaaac acgctgggcg atagcgtaga tgcgggtaac catccggcgg
    13981 tggcactgcg tactgatggc gacaaagtgc agatcaataa cgtcaacatt ctcggtcgtc
    14041 agaacacctt ctttgtcacc aacagcggtg tgcagaaccg tctggaaacg aatcgccagc
    14101 cgcgtacgct ggtgaccaac agctatattg aaggggatgt ggatatcgtt tctggtcgcg
    14161 gcgcagtggt gttcgataac accgaattcc gcgtggtgaa ctcccgtacc cagcaagaag
    14221 cgtatgtgtt tgcaccggct acgctgtcca acatttacta cggtttcctc gccgtaaaca
    14281 gccgtttcaa tgcttccggt gatggcgtgg cgcaactggg ccgctcgctg gatgttgatg
    14341 ccaataccaa cggtcaggta gtgatccgtg atagcgccat caacgaaggt tttaacacag
    14401 ccaaaccgtg ggctgatgcg gtgatctcta atcgcccgtt tgcgggtaac accggcagcg
    14461 ttgatgataa cgacgaagta caacgcaatc tgaatgacac taactacaac cgcatgtggg
    14521 aatacaataa ccgcggcgtg ggtagcaaag tggttgcaga ggcgaagaag taggagctgt
    14581 taactatgtg ccggatgcgg cgtaaacgcc ttatccggcc tacagttcga tacgatttgt
    14641 cggtcggata agatgcgcca gcatcgcatc cgacaataag tgccggatgc gacctacatt
    14701 cacatggcgc tttttacatc tgacggtttt tattgaagtt aatcaaacta cccgccttga
    14761 taatctcgcg ttcttcggca gtcagacttt ccatatagag cgtgatttcc gttaccggtg
    14821 catcttcatg gatcacataa cctttaaacg tcgttccagg attatccagc gccgctttaa
    14881 tgccagggat gtaaatgtaa tcccccactt caaaggttgg cgcttccgcc atctgcagcg
    14941 gtaacatccc ccagttgatg acgttagaac gatagcgttt agtcgcgtac tcctcggcaa
    15001 tattcgccag accgccaatc acacgctgac aactcgccgc ctgttcacgc gcagaaccat
    15061 cgcctggttt caccgcatag accatgctgc caatttcagt ttgcagcgga tcaacatgtt
    15121 cctgaccagc aatctgctta atgcgcgcaa acacctctgc catctcgctg acattccccg
    15181 ccagacgctg attttccagc tcagcagtcg ctttacttct gccaacataa cctggatcgc
    15241 ggcgtgacag ggtaaactcc gccagaccaa tcggatttga acgataagaa gaggtttcac
    15301 cggaaggaat cagttcgtcg gtggtggtca cttcgtcgag gatcttcgag cacactttga
    15361 ggacgatatt gtcagtcagc gcacccaatt ccggccagtc tttaatgttc ggtccgtaaa
    15421 tcagcggttg ctgggtcgcc cctttcacaa agccttgata gacgcgattt ttatacggcg
    15481 ttacatcgaa ggcgtactcc ggcacgttgt cccagcaatc gagttcgctg gcagaggtta
    15541 aatagccacc gtttgccgca gtcgcagcga tagaacgagc gtccatcaac gccaccgctg
    15601 acatctgccc attagctggc ttagagcctt cgcggttcgg gaagttacgc gtggtgtggc
    15661 gaatactcaa accgttgttg attggcgtat cgcccgcgcc aaagcatggg ccgcagaacg
    15721 cggttctgat gattgcgcct gcgccaatca aatctgcgac cacacctttt ttggcgagat
    15781 ccataaacac cggctgtgat gacgggtaaa ccgctaacga gaaggtgtca ttgccgcagg
    15841 attgaccgcg cagcgcattc gccgccgcga tgacgttttc atagttaccg ccagaacagc
    15901 ccgcgataat cccctgctgc actttcaggc gaccattttc cactttatcc agcagcgaga
    15961 gtttggcttt accgtgcgcc acgcgttcgg actcaatttc aatctcacgc agaatgtcgg
    16021 tcaggttctg gttcagcgtg tcgatttcat acacgttgct cgggtggaat ggcagcgcaa
    16081 tcattggttt gatggcgctt aaatcaacgc tgatgcagcc atcgtagtac gccattggtt
    16141 gagggttaag ttggcagtaa tcctggccgc gaccgtgcag cgccagccag ttatggactt
    16201 cttcatcggt ttgccagaca gaacttaaac aggtcgtttc agtggtcatc acgtcaacgc
    16261 tgttacggaa atcggtagag agcgctgcaa caccgggacc tacgaactcc atcactttgt
    16321 ttttgacgta accgtttttg aacacggcac cgatgatagc cagcgccaca tcctgcggcc
    16381 ccacatacgg cgcaggtttt ccggtcagat gcaccgcaac cacgcccgga tagtcgatat
    16441 cccaggtgtc attaagcagt tgttttacca gctcgccgcc gccctcaccg actgccattg
    16501 tccctaatgc gccgtaacgg gtgtggctgt ctgatccgag gatcattttg ccgccgccag
    16561 ccatcatttc acgcatatat tgatggatga ccgcaatatg cggtggcaca aaaataccgc
    16621 cataacgctg ggccgccgat aaaccaaaaa catggtcatc accgttaatg gtgccgccga
    16681 cggcgcagag tgagttatgg cagttggtca gcacatacgg cagcgggaaa cgttccatac
    16741 cggacgcttt agcggtctgt acaataccaa caaaggtaat atcgtgagag gctaatgaat
    16801 caaacttaat tttaagttta tccatatttc cggacgtatt atgagaagag agaatagacc
    16861 aggcaatagt gccttttttg gcttcttctt ttttaatttc gccggtgaaa tgttcttcgg
    16921 caattatttc gttattactg gcgagaaaca cgcctttttc agataacttg atcatcaaat
    16981 gctccagata tctgcgcacc ggaaagtgcg cagaaagata ataaatggat atactctaaa
    17041 taattcgagt tgcaggaagg cgacaagcga gtgaatcccc agtcacttac ataagtaagt
    17101 gactggggtg agcgaacgca gacgcagcac atgcaacttg aagtatgacg agtatattta
    17161 cagcatgttc cagccgatca gcatattcca ccaccacacg ccgagggtga tatgcaccag
    17221 gaaggttaat atcgtcagta ccgcaccgac caaccaccag gatttaatat cgttgtaacc
    17281 cacgccaaag atgaccggac ctgccgcgcc gccatagtga gtaaccatgc cgccatagga
    17341 gttagagaac aacagtgcca gcgcggttaa cattaacggt gcgccggaga cattcgccag
    17401 catggcaaat accggtaaca tggcaacgat ataggcacta ccagaagcga agaaataacg
    17461 cacgatgatg ctgaggaaaa taataacgaa gaaagcaacg ttcccgtgac catcaaatgc
    17521 caggttattt ttaaagactt cagctaacca ttcgaagaat ttaactttcg ataataagga
    17581 gcttaagccg ataataccgc cgtaccagat taaggtattc cagccgcctt tatttttaac
    17641 cacgtcttcc caggtaacga tacccagcag cagcatagtt gccataacaa cgattgccac
    17701 ggtggattca tcaaccccca gagacttact gaaaatccag cccagcagcg ccagcacaaa
    17761 gacaccgagc agcatttttt cgcggatttt catcggtccc agttcggcaa ggcccgcttt
    17821 agcgatggtt ttgttatcca ccttcttaat ttctggtgga tacatggtgt aaatcaccag
    17881 cggggtgacc agcagcataa tgatgcccgg caatccggcg gctaacgccc atccacccca
    17941 gctaatttgc aggtgcagaa tgtcgttgat cattttcagc gccagaatgt tccccgccat
    18001 tgcggtaaag aacatatagc tggtggtttt ggtgaccatg taaatggaca tcatcaggta
    18061 atgtccaaca cgacgcggac ttttttccgg ttctgatccc aaagccactg ccacgctgtt
    18121 gatgatcggt aacacgatgc cgcccgcacg cgcggtgtta gacggtgttg ccggagccag
    18181 taccagatcg agaaataccg taacgtaacc cagacccagg gtagtgctac caattttacc
    18241 aatcagcaga taggcaatac gtttacctaa gcctgtggtt acaaacgcgg cgcttaaagt
    18301 aaacgcagaa aacaccagcc aggtggtacc ggaagagtaa ccgcttaata cagcggtggt
    18361 tttaaattcc ccacctgata agttacccac cactaccatc gatgcggcga cggcaattaa
    18421 cagtacgaca ggttccggga aaggcttgat aaccagcccc acaatggccg ccaggtaaat
    18481 accaaaaagc acccacgcca gttcgcttaa tcctgccgga gctggcataa agccaataat
    18541 acatgggatt gctaatatca gaattagctt ccataacgat ttcttattca tttaatatcc
    18601 tgttctttct gtatgactca tacagaattc ctttattgat tgtcattatc acgacaacaa
    18661 aatttttcag ggaagataaa cttcaccgga aaatattttt ctggtcgtcc gaataacaga
    18721 tgcgcgtaac gtcgtggcat cctgaccttc attacttaaa tgaacgtcga gcgcaccact
    18781 ggggtgttca atattgatat tgccgtatcc tacagaaggg acgatttgtc gggtgacggt
    18841 accttccaat gcacaactac tggaaatagc aatagcaccg gttatcgcca aagcgcgatg
    18901 gcaagaatgc ggcataaaat aacgcacatt aattgccccg cctttctgcg ctggagaaat
    18961 aagtacaggt ttagggataa ccatattact gacatcgcct aaacccattg ctttacccgc
    19021 ttgtagacgg atagattcaa tgcgggctaa taatgctttg tcggcatcca gctctgccgg
    19081 taattcatag cctgtttttc ccagatattc agcaggaata atgacaactg gcatcgccat
    19141 atcgatacag gtcaccggga catcgtcaaa ataatcaatc tgattatccg tcgggaagac
    19201 ttttccggtt ttcgttccgg cggcattcag gaaagtgagc gcaaccggtg cggcagtacc
    19261 cggtacgccg tcaattctgg cgctaccctc gtactcgaca acaccatttg gcgtttgcac
    19321 atcagcttcg atgaacgtac ccgtattgac gttgcggata cgtacgcggg taactggcga
    19381 agtcgctgca atcaaaccat tttcaatggc gaatgcccca acgccagaca gcatattgcc
    19441 gcagttaggc gtggtatcga cacgttgctc atggacgata acttgagcaa acagataatc
    19501 gacatctgca cgcggatcgc tggagcggct aataatggcg actttactgg tcagcggatt
    19561 accgccgcca ataccgtcaa tttccagatc gttaccggaa cccataattg ccatcaatat
    19621 tttatcgcgc tgcgtttgat cttcgggtag atgttccgct aacaggaacg cgcccctcga
    19681 ggttccacct cgcatcatca cgcagggtat ttttttcata ataatgccct caatttaaaa
    19741 acataactta ttgcgatatg gttacattaa gggcaaagca tctctaatga ctttagctca
    19801 acgattaata acattttttc attaatttaa gttggctaaa tgcagttaaa atttttaact
    19861 gccagtcacc caaaatcgtc gcttccattt tccggagtaa taatgaagca tgaattatca
    19921 agtatgaagg catttgtcat actggcagag tccagttcat ttaacaatgc tgctaaatta
    19981 ctcaatatta cgcaacctgc attaacgcga agaataaaaa agatggagga agatttacac
    20041 attcaactat ttgagcgtac aactcgcaag gttacgttaa caaaagcagg aaaaaggttg
    20101 ctcccggagg cgcgggaatt aataaaaaaa ttcgatgaaa cgctttttag tattcgcgac
    20161 atgaatgctt atcatcgagg tatggtgacg ttagcgtgta ttccgaccgc ggtattttat
    20221 tttttaccat tggcaatcgg taaatttaat gagctgtatc ccaatattaa agtgcggatt
    20281 ctggaacaag gcacgaataa ttgcatggaa tcggtgctgt gtaacgaatc agactttggc
    20341 ataaacatga acaacgtcac aaattcatcc attgatttta ccccactggt caacgagccg
    20401 ttcgtgctgg cctgtcggcg tgaccatcca ttggccaaaa agcagcttgt agaatggcag
    20461 gagttagtgg gttacaaaat gattggcgta cgctcctcca gcggcaaccg actgttgata
    20521 gaacaacagc tagccgacaa gccctggaag ctggactggt tttacgaagt gcgtcatctt
    20581 tcgacgtcat tagggctggt ggaggcggga ctggggattt cggcgcttcc tggtctggca
    20641 atgccccatg cgccctattc ttcgattatt ggtatcccac tggtggaacc cgttatacgt
    20701 cggacattgg ggattattcg ccgcaaagat gccgtgcttt ctccggcagc tgaacgcttt
    20761 tttgccttgc tgattaattt gtggactgac gataaagaca atttgtggac caatatcgtc
    20821 gaacgccaga gacatgcgtt acaggagata ggttaataca ccggcgcagg agagactgcg
    20881 ccggttaaat cagcggttag tgcgcgttga ccactaccca cattggcccc tgcccaaccg
    20941 catagcggcc tttttcatgc agtagcccct gctcgccaac aatttcgtat accgagatgt
    21001 ggtgagattt ttgcccggcg gcaatcagat acttgccgct gtgatcaaca ttgaagccgc
    21061 gcggctgggt ttccgttggc tggaagcctt ctttactcaa cacgctgcca tcttccgaaa
    21121 cgctgaaaac ggtaatcaga ctggcggtac ggtcgcaggc gtataaatga cgaccatccg
    21181 gggtgatatg aatatcagcc gcccaacggg tgtcggagaa gttttccggc atcatatcca
    21241 gcgtctggac acattcgata ttgccgtgcg gatctttcag ttcccagaca tccactgagc
    21301 tgtttaactc attgacgcaa tacgcatatt gttcgtttgg atggaatacc atatgacgcg
    21361 ggccggcccc ttcaacggta gtcacttccg cagggtcctg cgccaccaga tgaccatcat
    21421 cgctgaccgt aaacaggcaa atgcgatcct gctttaatgc cggaacccac aacgtacggt
    21481 tgtccggtga gatattggcg gaatggcaac cgtccagccc ctcgaccaca tcgacgacgc
    21541 ccactggcag gccatcttcc agacgcgtta cgctcacgtt acccgcattg taagaaccga
    21601 caaagacaaa ctgcccctgg tgatcggtgg aaatatgcgt cgggctaccc ggcagcgcag
    21661 actctgcagc aaaggtcagc gcgccatcat ccggtgcaat acgatacgcc agaacacgaa
    21721 actccggacg gacaccaacg tagaggtaac gtttgtccgg gctgaccacc atcggctgca
    21781 cctgccccgg cacatcgaca acctgtgtca gcgtcagtgc gccttcatga ttcaggttcc
    21841 agacgtgaat ttgctggctc tcagggctgg cgatataaac tgtttgcttc atgaatgctc
    21901 ctttgcattt agcaactgac tgtaacagct aaaattagtc gcttttggcg gtgaatgctg
    21961 aattaaaacg cagaattttg tccgatagtg gtatcggtgt accattcgcg cagacgaaaa
    22021 ttcttaacct taatctggaa tacgccatga ccacacgcgt gattgctctc gacttagacg
    22081 gcaccttatt gaccccgaaa aagaccctgc ttccatcatc gatagaagcc ctggcccgcg
    22141 ctcgcgaagc aggctatcga ttaatcatcg tcacaggtcg ccatcacgtc gctattcatc
    22201 ctttttatca ggcgctggcg ctggatacac ctgctatttg ctgtaatggc acctatttgt
    22261 atgattatca tgcaaaaacc gtgctggaag cggacccaat gcccgttaat aaagccctgc
    22321 aactcattga gatgctgaat gaacaccaca ttcacggtct gatgtatgtg gatgatgcga
    22381 tggtctatga gcacccgacc ggacatgtca ttcgcacgtc taactgggcg caaaccctgc
    22441 cgccggaaca gcgtccgact ttcacacaag tcgcttctct ggctgaaacg gcgcaacaag
    22501 ttaacgccgt atggaagttc gccctgacgc acgatgacct gccgcaattg cagcattttg
    22561 gtaagcatgt cgaacatgaa ctgggactgg agtgtgaatg gtcctggcac gatcaggttg
    22621 atattgcacg cggcggcaac agcaaaggta aacgtttgac gaaatgggtt gaggcgcaag
    22681 gctggtcgat ggaaaacgtc gtggcgttcg gcgataactt caatgatatt agtatgctgg
    22741 aagccgctgg tacaggcgtg gcgatgggca acgccgatga cgcagtaaaa gcgcgcgcca
    22801 acattgtgat tggtgataac accaccgaca gcattgccca gttcatttat agccacctga
    22861 tttaatcagg cggttatcga cacactttta atttgcgcat acagccacag gccaggtttg
    22921 atcgccagtt catccctggc ccacgggctg atacgcgccc acagcgtttt acagccgact
    22981 tccagctcca cttccacctg gccgttgtcg tcataactat taacaacttt tgcccgcaat
    23041 acgttacgaa tgctggtttg ctgcggcggt tgtaaaacca gtgagacatc ggaagcctga
    23101 atgcggatgc gtagcgcagc ttgcagcggt tcgtccagct tattgaccca caaatgctga
    23161 tcgcccagcg ccagcgcggt catcgcgtaa tgcggatgat gctcaagtac tgtcactttc
    23221 agaatgctac tttgttgctc tttcggtagc cacggattca tcacgctact accccagact
    23281 tcttccagcg cgccaaaggc tttcacctga ccgttttcca gtaccatcac tctgtctgcc
    23341 agatggagga tctcatccag cgaatggctg acatacaaca tcggaatgtt gatttcccgc
    23401 gtcagacgtt gcagataagg caacagttcg cgtttacgcg gaatatccag tgatgccagc
    23461 ggttcatcca gcaacagcaa ttccggtgct gtcagcaaag cccgaccaat cgccacgcgc
    23521 tgtttttcgc ctccggacag gctgcctggt aaacgatcaa gcaacggttc aatgcctaaa
    23581 agcgccacca gcttatcgaa ctgattgacc atgcttttcg ccatgccgta gcgcaaattg
    23641 ccacgcactt tataatgcgg aaacagccgc gcatcctgaa acacatagcc tacgcgacgc
    23701 ttttctggcg tcaggcagat acctttatcg gcgtcattta gtacccgacc attgaggaca
    23761 atccgccctt tttgcggacg tgtcagccca ctgatggcgt taatcagcga ggtttttccg
    23821 gcaccagaga caccaaaaat agcggtgatg ccattggcgg gcagcgtttc attaatcgtc
    23881 aggcaatggt tgcccaacgt ctgggaaaaa ttcagttcca gcatgattag cgccccgccc
    23941 gctcacggct gattctggcc agccattctg aaatcaacag ggagatcatc gccagcgcaa
    24001 tagaaataat acacagtctc gccgcgccac tttccccgcc aggggtctgg atcagggtat
    24061 acatggcaga aggaatggtc cgcgtttcac caggaatgtt cgacacaaag gtgatggttg
    24121 cgccaaattc accgagcgaa cgagcgaatg ccagtaccgt accaacaata attcccggta
    24181 aggtcagcgg taacgtgata gtaaagaaaa cgcgccagcg cccggccccc agtgttcttg
    24241 cggcctgctc cagtttgata tcaacacctt ccagcgccag acgaatcgcc cgcaccatca
    24301 gcggaaaaga catgacagcg gcagcgagaa ccgcaccacg ccagctaaac gcgaaagaaa
    24361 gaccaaacca gtcatacaga cgttcaccga taaatccgcg ccgtcccatc gaaactaata
    24421 ataggtaacc gacgaccacg ggcggtaaca ccagcggtag atgcagtacg ctgtcgagca
    24481 gagctttgcc cggaaacgtg caacgtacca gtaaccaggc aaaaaagatc ccaaacggca
    24541 ggctaaacag cacagccagg gaagaaactt tcaggcttaa taaaactgcc tgccattctg
    24601 gatcggtcag tatcattact tggttgtaaa tccgtaacgt ttaaagattt ccgcagcctg
    24661 cggccccttc agataatcat aaaatgcttt cactgtcgca ttgttatgcc cttccacaac
    24721 agcaaccgga tattccactt ttttatgtga atcttccggg aaggtggcaa ccacttttac
    24781 ccctttgctg gcaactgcgt cagaaccgta aacaataccc agcggcgctt cattgcgttc
    24841 gaccaacgcc agcgcgccac gaacatcttc cgccggagcc agtttcgggg aaagcgtatc
    24901 ccatgcgccc agtttttgca gtgcttcttt cgcataaatg ccagcaggga catgttccgg
    24961 atcaccaacc gcgaggcgac cgccattcag cagcgaagtc cagttggttt tgctgtcgat
    25021 ggtgaaatct ttctgctcgc tggctttcgg tgctacaacg accaggctat tgccgagcag
    25081 tgtctggcgc gaagccgtat cgatcgcttt tttatcaacc gcataatcca tccatttctg
    25141 atcggcagaa ataaacagat ccgcaggcgc acccgcttca atctgacggg ccaaagtaga
    25201 tgacgaagcg aaagaagaaa ccacatccac gcctttctct tttttatact gcgtagcaat
    25261 gtcctgcatt gcgttagtca gtgatgcggc ggcgaacacc gtgattttcc cttcatctgc
    25321 cagtgcattg ccagcaacag cgaaagagag tgccgccccg gtaaacaagt tcaaccattt
    25381 acgagccatt ggtaacccct taatctaacg ttatgtaggc gtcaatataa cggcaagttc
    25441 ggaattttcc cagcagttat tgctaacctt tgtgaggtag atgagaaaaa atatcggcag
    25501 aaaaagcagg aagttgagaa aaagaaaatg cccgactaag cgggcattca gggaatcaat
    25561 gattttgtcc gggctggtct tttttaccaa caccagaaaa gatgttgaat acttcaccaa
    25621 gaccgtaaat cagacccagg atgatggcca tcacgacagg taccatgatt acggcgaata
    25681 ccagactttt taataactct aacatggtca actccagata tagtcatgag actattctaa
    25741 ccgctaagca cagaaaagca ctcccctttt gtgcggtcag ctttgcgtgg cgttcgtttt
    25801 ccgtcacaat aagacttttg ccaggacatt gttatgcagg ccgaaatcct tctcaccctt
    25861 aagctccaac aaaaattatt cgccgacccg cgccgcattt cgctactaaa acacattgcg
    25921 ctttccggtt ccattagcca gggagcgaaa gatgccggta tcagctataa aagcgcctgg
    25981 gatgccatta acgagatgaa tcagttaagt gagcatattc tggttgagcg cgcaacaggc
    26041 ggtaaaggtg gaggcggcgc agtactgacc cgctatggtc agcgactgat tcaactctat
    26101 gacttactgg cgcaaatcca gcaaaaagcc tttgatgtgt taagtgacga tgacgccctg
    26161 ccgctgaaca gcctgctggc cgcgatctca cgtttttcac tgcaaaccag cgcccgtaac
    26221 cagtggttcg gtaccatcac cgctcgcgat catgatgacg ttcaacagca tgttgatgtc
    26281 ttactggctg atggaaaaac acgcctgaaa gtcgcaatta ccgcacaaag cggcgcgcgt
    26341 ctggggctgg atgaaggcaa agaagtgttg atattgctaa aagcgccgtg ggtaggtatt
    26401 actcaggacg aggcggtcgc gcaaaacgct gacaaccaat taccgggtat tattagtcat
    26461 attgagcgcg gcgcagagca gtgcgaagta ttaatggcgc tacccgacgg gcaaacactg
    26521 tgcgccacag tgccggtaaa tgaagcgact tctcttcagc aagggcagaa tgtcacggcc
    26581 tactttaatg ccgacagcgt gattatcgcc acgctgtgct aagcgtgttg acaatttgtt
    26641 atgaaacacg tatccctgtc agtaatcgct gcacaaaatg ggatataaaa tgtcatcgtt
    26701 gcaaattttg caaggcacgt ttcgtcttgg cgacacaaaa acgctgcaat tgcctcggct
    26761 aacgttaaac gcgggtgata gttgggcgtt tgtcggttcg aatggaagcg ggaaatcggc
    26821 gctggcccgc gcgctgtcgg gggaacttcc gcttttgaaa ggtgaacggc aaagccagtt
    26881 ttcccacatc actcgtctct ccttcgagca attgcaaaag ctcgtcagcg acgaatggca
    26941 gcggaataac accgatatgc ttagtcctgg cgaagatgac accgggcgta ccacggctga
    27001 gatcattcag gatgaagtaa aggatacttc gcgttgcatg caactggcgc agcagttcgg
    27061 tattaccgcc ctcctcgacc gacgctttaa atacctttcc actggcgaga cgcgaaaaac
    27121 cctgctgtgt caggcgctga tgtcggagcc tgacttgttg atccttgatg aaccgtttga
    27181 tggcctggat gttgcctcac gtcagcatct ggctgagcta ctcgcctcgt tacatcagtc
    27241 cggtattact ctggtactgg tgctcaatcg cttcgatgag atcccggaat ttgtccagtt
    27301 tgctggcgtg ctggcggatt gcacgttagc ggaaactggc gctaaagaag aactgctcca
    27361 acaagcactc gttgcgcaac tggcgcatag cgaactgctt gaaggtgtgc aaatgccgga
    27421 gccggatgaa ccttcagcac gtcacgcctt acccgccaac gaaccgcgca ttgtgctgaa
    27481 caatggcgtg gtttcttata acgaacgccc catccttaat aaccttagct ggcaggtgaa
    27541 tccaggcgaa cactggcaaa ttgtcgggcc aaatggcgcg ggaaaatcga cgttattaag
    27601 cctgattact ggcgatcatc cgcaaggtta cagcaacgat ttgacgcttt tcggacgacg
    27661 tcgcggcagc ggcgaaacca tctgggatat caaaaagcat atcggttacg tcagcagtag
    27721 tttgcatctg gattaccggg tcagcactac tgtgcgtaat gtgattcttt ctggctattt
    27781 tgattcgatt ggcatttatc aggccgtttc ggatcgccag caaaaactgg tgcagcagtg
    27841 gctggatatt ctcggcattg ataaacgcac ggctgacgct cctttccata gtctttcctg
    27901 gggacagcag cgtctggcgc tgattgtccg cgcactggtg aaacatccga cgttgcttat
    27961 tctcgatgaa ccactacagg ggcttgatcc gctcaatcgc cagcttatcc gccgttttgt
    28021 tgatgtgctg attagcgaag gtgaaacgca attgttgttt gtttcgcacc acgctgaaga
    28081 tgcgcctgcc tgtattaccc atcgtcttga gttcgtgccg gacggtggac tctatcgcta
    28141 tgtgctgaca aaaataaatt gagtcggtaa tgctgaccgt gccggaggcg gcgtaaacgt
    28201 gttatccggc caacggtttg acgcatccag gcatgaaacc gcgtcttttt tttcagataa
    28261 aaagcgcaat cattcataaa gccgctgttt tataatcact taatcgcgca taaaaaacgg
    28321 ctaaattcct gtgtaaacga ttccactaat ttattccatg tcacactttt cgcatctttg
    28381 ttatgctatg gttatttcat accataagcc taatggagcg aattatgaga gttctggtta
    28441 ccggtggtag cggttacatt ggaagtcata cctgtgtgca attactgcaa aacggtcatg
    28501 atgtcatcat tcttgataac ctctgtaaca gtaagcgcag cgtactgcct gttatcgagc
    28561 gtttaggcgg caaacatcca acgtttgttg aaggcgatat ccgtaacgaa gcgttgatga
    28621 ccgagatcct gcacgatcac gctatcgaca ccgtgatcca cttcgccggg ctgaaagccg
    28681 ttggcgaatc ggtacaaaaa ccgcttgaat attacgacaa caatgtcaac ggtactctgc
    28741 gcctgattag cgccatgcgc gccgctaacg tcaaaaactt tatctttagc tcctccgcca
    28801 ccgtttatgg cgatcagccc aaaattccat atgttgaaag cttcccgacc ggcacaccgc
    28861 aaagtcctta cggcaaaagc aagctgatgg tggaacagat ccttaccgat ctgcaaaaag
    28921 cccaaccgga ctggagcatt gccctgctgc gctacttcaa cccggttggc gcgcatccgt
    28981 cgggtgatat gggcgaagat ccgcaaggca ttccgaataa cctgatgccg tacattgccc
    29041 aggttgctgt aggccgtcgc gactcactgg cgatttttgg taacgattat ccgaccgaag
    29101 acggcactgg cgtgcgcgat tacatccacg taatggatct ggcagatggt cacgtcgtgg
    29161 cgatggaaaa actggcgaac aagccaggcg tacacatcta caacctcggc gcaggcgtag
    29221 gcagcagcgt gctggacgta gttaatgcct tcagtaaagc ctgcggcaaa ccggttaact
    29281 atcattttgc accacgtcgc gcgggcgacc ttccggccta ctgggcggac gccagcaaag
    29341 ccgaccgtga actgaactgg cgcgtaacgc gcacactcga tgaaatggcg caggacacct
    29401 ggcactggca gtcacgccat ccacagggat atcccgatta aggaacgacc atgacgcaat
    29461 ttaatcccgt tgatcatcca catcgccgct acaacccgct caccgggcaa tggattctgg
    29521 tttcaccgca ccgcgctaag cgcccctggc agggggcgca ggaaacgcca gccaaacagg
    29581 tgttacctgc gcacgatcca gattgcttcc tctgcgcagg taatgtgcgg gtgacaggcg
    29641 ataaaaaccc cgattacacc gggacttacg ttttcactaa tgactttgcg gccttgatgt
    29701 ctgacacgcc agatgcgcca gaaagcaacg atcctttgat gcgttgccag agcgcgcgcg
    29761 gtaccagccg ggtgatctgc ttttcaccgg atcacagtaa aacgctgcca gaactgagcg
    29821 ttgcggcatt gacggaaatc gtcaaaacct ggcaggagca aaccgcagaa ctggggaaaa
    29881 catacccgtg ggtgcaggtc tttgaaaaca aaggcgcggc gatgggctgc tctaacccgc
    29941 atccgcacgg acagatttgg gcaaatagct tcctgcctaa cgaagctgag cgcgaagacc
    30001 gtctgcaaaa agaatatttc gccgggcaga aatcaccaat gctggtggat tatgttcagc
    30061 gcgagctggc agacggtagc cgtaccgttg tcgaaaccga acactggtta gccgtcgtgc
    30121 cttactgggc tgcctggccg ttcgaaacgc tactgctgcc caaagcccac gttttacgga
    30181 tcaccgattt gaccgacgcc cagcgcagcg atttggctct ggcgttgaaa aagctgacca
    30241 gtcgttatga caacctcttc cagtgctcct tcccctactc tatgggctgg cacggcgcgc
    30301 catttaatgg cgaagagaat caacactggc agctgcacgc gcacttttat ccgcctctgt
    30361 tgcgctctgc caccgtacgt aaatttatgg ttggttatga aatgctggca gaaacccagc
    30421 gagacctgac cgcagaacag gccgcagagc gtttgcgcgc agtcagcgat atccattttc
    30481 gcgaatccgg agtgtaagaa atgagtctga aagaaaaaac acaatctctg tttgccaacg
    30541 catttggcta ccctgccact cacaccattc aggcgcctgg ccgcgtgaat ttgattggtg
    30601 aacacaccga ctacaacgac ggtttcgttc tgccctgcgc gattgattat caaaccgtga
    30661 tcagctgtgc gccacgcgat gaccgtaaag ttcgcgtaat ggcagccgat tatgaaaatc
    30721 agctcgacga gttttccctc gatgcgccca ttgtcgcgca tgaaaactat caatgggcga
    30781 actacgttcg tggcgtggtg aaacatctgc aactgcgtaa caacagcttc ggcggtgtgg
    30841 acatggtgat cagcggcaat gtgccgcagg gtgcagggtt aagttcttcc gcttcactgg
    30901 aagtcgcggt cggaaccgta ttgcagcagc tttatcatct gccgctggac ggcgcacaaa
    30961 tcgcgcttaa cggtcaggaa gcagaaaacc agtttgtagg ctgtaactgt ggaatcatgg
    31021 atcaactgat ttccgcgctc ggcaagaaag atcatgcctt gctgatcgat tgccgctcac
    31081 tggggaccaa agctgtttcc atgccgaaag gcgtggctgt cgtcatcatc aacagtaact
    31141 tcaaacgtac cctggttggc agcgaataca acacccgtcg tgaacagtgc gaaaccggtg
    31201 cgcgtttctt ccagcagcca gctctgcgcg atgtcaccat tgaagagttc aacgctgttg
    31261 cacatgaact ggacccgatc gtcgcgaaac gcgtgcgtca catcctgact gaaaacgccc
    31321 gcaccgttga agctgccagc gcgctggagc aaggcgacct gaaacgtatg ggcgagttga
    31381 tggcggagtc tcatgcctct atgcgcgatg atttcgaaat caccgtaccg caaattgaca
    31441 ctctggtaga aatcgtcaaa gctgtgattg gcgacaaagg tggcgtacgc atgaccggcg
    31501 gcggatttgg tggctgtatc gtcgcgttga tcccggaaga actggtgcct gccgtacagc
    31561 aagctgtcgc tgaacaatat gaagcaaaaa caggtattaa agagactttt tacgtttgta
    31621 aaccatcaca aggagcagga cagtgctgaa cgaaactccc gcactggcac ccgatggtca
    31681 gccgtaccga ctgttaactt tgcgtaacaa cgcagggatg gtagtcacgc tgatggactg
    31741 gggtgcgact ttactttccg cccgtattcc gctttccgat ggcagcgtcc gcgaggcgct
    31801 gcttggctgc gccagcccgg aatgctatca ggatcaggcc gcatttctgg gggcctctat
    31861 tggtcgttat gccaaccgca tcgccaatag ccgttatacc tttgacggtg aaaccgtgac
    31921 gctttcacca agtcagggcg tcaaccaatt gcatggcggc ccggaagggt tcgacaaacg
    31981 tcgctggcag attgtgaacc agaacgatcg tcaggtgctg tttgccctga gttcagatga
    32041 tggcgatcag ggcttcccgg gtaatctcgg cgcgacggtg caatatcgtc tgaccgacga
    32101 taaccgtatc tccattactt atcgcgccac agttgataaa ccttgcccgg tgaatatgac
    32161 taatcacgtc tatttcaatc ttgacggcga gcagtctgac gtgcgcaatc ataagttgca
    32221 gattctggcg gaagaatatc tgccggttga tgaaggcggc attccgcacg acggcctgaa
    32281 atctgtcgcc ggaacctctt ttgatttccg caacgccaaa atcatcgcca gtgagtttct
    32341 tgccgacgac gatcagcgca aagtgaaagg ttacgatcac gcgttcttgt tacaggccaa
    32401 aggcgatggc aagaaagtgg cggcgcatgt ctggtcagca gatgaaaaat tgcagctgaa
    32461 ggtctacacc accgctccgg ctctgcaatt ctactccggc aacttcctcg gcggcacacc
    32521 gtcgcgggga accgaacctt acgctgactg gcaagggctg gctctggaaa gcgaatttct
    32581 gccggacagc ccgaaccacc ctgaatggcc gcaaccggac tgcttcctgc gtcctggcga
    32641 agagtattcc agcctgacgg aatatcagtt tattgctcag taatgttaag ccctcgtcat
    32701 gagggcttta tcctcatatt gttcaaatca ccagcaaaca ccgacatatt tgcaactcaa
    32761 tattcacaac aaccttacac tgcgacacta ttttcgctat ggttatgcgt aagcattgct
    32821 gttgcttcgt cacggcaata taatgagaat tattatcatt aaaagatgat ttgaggagta
    32881 agtatatggc tgtaactaag ctggttctgg ttcgtcatgg cgaaagtcag tggaacaaag
    32941 aaaaccgttt caccggttgg tacgacgtgg atctgtctga gaaaggcgta agcgaagcaa
    33001 aagcagcagg taagctgctg aaagaggaag gttacagctt tgactttgct tacacctctg
    33061 tgctgaaacg cgctattcat accctgtgga atgtgctgga cgaactggat caggcatggc
    33121 tgcccgttga gaaatcctgg aaactgaacg aacgtcacta cggtgcattg cagggtctga
    33181 acaaagcgga aaccgctgaa aagtatggcg acgagcaggt gaaacagtgg cgtcgtggtt
    33241 ttgcggtgac tccgccggaa ctgactaaag atgatgagcg ttatccgggt cacgatccgc
    33301 gttacgcgaa actgagcgag aaagagctgc cactgacgga aagcctggcg ctgaccattg
    33361 accgcgtgat cccttactgg aatgaaacca ttctgccgcg tatgaagagc ggtgagcgcg
    33421 tcatcatcgc cgcacacggt aactcgttac gtgcgctggt gaaatatctc gataacatga
    33481 gcgaagaaga gattcttgag ctgaatatcc cgaccggcgt gccgctggtt tatgagtttg
    33541 acgagaactt caaaccgctg aaacgctatt atctgggtaa tgctgacgag atcgcggcga
    33601 aagcagcggc ggttgctaac cagggtaaag cgaagtaaac gtcattcgct taaaaatgag
    33661 aaagccgact gcaaaatgag tcggcttttt tatttttcgt tgctaccaat ggagcgcatt
    33721 gcctgatgcg acgctgcgcg ttttatcagg cctacggtga ttaatcggat acgccactct
    33781 gacagcgcat ccgacaatta aactttaccc gcgacgcgct tttactgcat tcgccagttg
    33841 acgtaacaga gcatcggtat catcccagcc aatgcaggca tcggtgatgc tcttaccgta
    33901 ggccagcggt tccccgctct cgaggctctg attgccttcc accagatggc tttccaccat
    33961 cacgccaata atggcctttt cgccaccggc aatctgctgg caaacgtcag cacaaacatc
    34021 catctgcttt ttgaattgtt tagacgagtt agcatggctg aaatcgatca tcacctgtgc
    34081 tggcaggcct gctttgttca gcccttcttt cacttcagca acgtgcttcg cgctgtagtt
    34141 aggctcttta ccgccgcgca gaatgatatg gcaatcgccg ttaccgctgg tattcacaat
    34201 cgccgaatga ccccatttgg tcactgaaag gaagcagtgc ggcgcaccgg cggcattaat
    34261 ggcatcgata gccactttaa tcgtaccatc agtgccattt ttgaagccta ccgggcaaga
    34321 aagccctgaa gccagttcgc ggtgcacctg cgattccgta gtacgtgcgc caattgcgcc
    34381 ccagctcatc aggtcagcga gatattgtgg ggtgatcata tcgagaaact cacccgccgc
    34441 tggcagaccg ctgtcgttaa tatcaagcaa caatttacgg gcaatacgca gaccgtcgtt
    34501 gatctggaag ctgttatcca tatgcggatc gttaatcagc cctttccagc ccaccgtggt
    34561 acgcggcttt tcaaaataga cgcgcattac gatttccagc tcatctttca gctcttcacg
    34621 cagcgccagc aagcgagtgg catactcttt agccgcgaca ggatcatgaa ttgagcatgg
    34681 gccaatcacc accaacaggc gatcatcatt acctttcagg atcttatgga tcgcttttcg
    34741 ggcatgggcg accgtattcg cggcattttc agtagcgggg aatttttcca gcaatgcgac
    34801 aggaggaagt aactctttga tttctttgat gcgtaaatcg tcgttctgat aattcatgtc
    34861 tgttccagtg ttgccatact tatcttagtg aatgcaatac ttccaatcta tatcttccgt
    34921 cagaatgtgt aaacggggtt ttacactatg aacggattaa tcctggaatt agcaagaaaa
    34981 acgccagatt gtcgcgaaaa acgagatctc tcctacaatt tctaactgta actcctttgt
    35041 ttgttaatta tttcaagatt ctctgctggg tatctttgcc tggctgaaat cttaaaccat
    35101 tgccttatat tcacctgcaa atgcactgtt ggaagaggtt atccgacata acgaccataa
    35161 caggagcatc ctatgaaaat gacaaaactg gccacactat ttttgactgc cactctaagc
    35221 cttgccagcg gtgccgcact ggctgccgat agcggagcgc aaactaataa cggccaggca
    35281 aacgccgccg ctgatgcggg ccaggtagcc cctgatgccc gtgaaaatgt cgcgccaaat
    35341 aacgtcgaca ataacggggt aaataccggt tctggcggca caatgctgca tccggatggt
    35401 tcttcaatga acaatgacgg catgaccaaa gatgaagagc acaagaacac catgtgcaaa
    35461 gatggtcgct gcccggatat caataaaaaa gtccaaaccg gtgatggcat caacaatgat
    35521 gtcgatacca aaaccgacgg taccacgcag taaccctgcg aaatagccaa taatgggaga
    35581 gcgtagtctc tcccttttaa tttttccctt ctaaaagcag tatgatgtca taaagttaag
    35641 tgagaataat taagggatga ctcgtcatgg cgcactcaca ctcacacact tcctcacacc
    35701 tgccagaaga taataatgct cgtcgcttgt tgtatgcttt cggcgtgact gccgggttta
    35761 tgctcgtaga agtcattggt ggttttcttt ccggttctct ggcattgctg gccgatgcgg
    35821 gtcatatgtt gaccgatact gccgccctgc ttttcgccct actcgcagtg caattttccc
    35881 gtcgtcctcc caccattcgc cacactttcg gttggctaag attgaccacg ctcgcggcgt
    35941 ttgtgaatgc catcgccctg gtggtgatta ctattttgat tgtctgggag gcgatagaac
    36001 gcttccgcac tccgcgtccg gtcgagggcg gcatgatgat ggcaattgcc gtggctgggc
    36061 tgctggcaaa tatactttct ttctggttac ttcatcacgg cagcgaagag aaaaacctca
    36121 acgtgcgagc agcggcactg catgtgctcg gggacctgct gggttcggtt ggggctatta
    36181 ttgccgcgtt gattattatc tggaccggct ggacacctgc tgaccccatt ctctcaatac
    36241 tggtgtcgct tctggttctg cgtagcgcgt ggcgactgtt gaaagatagt gtgaatgaat
    36301 tacttgaagg tgcaccggta tcgctggata tcgctgaact gaagcgtcgc ttgtgccggg
    36361 aaatcccgga agtccgcaat gtgcaccatg tgcatgtatg gatggtaggc gagaagccgg
    36421 tgatgacgct gcatgtgcag gtgatcccgc cacacgatca cgatgccttg ttggatcaga
    36481 tccaacacta cctgatggat cactatcaga ttgagcacgc caccattcag atggaatatc
    36541 aaccttgtca tgggccagac tgccacctga atagaggggt gtctggccat tcacatcatc
    36601 atcattaatg ggacagcgca cgtgaacctc tttcgcgtgc gcttttgatc cacatccggc
    36661 tgccgttgag ggcaataaag gtcaggatga tgtactccag agacatggcg taaacgccct
    36721 gaagtgcaaa aataacgacg ctaatcacgt taataatcac ccacaacagc cagttttcca
    36781 catacttacg tgtcatcaga atcattgcca cgatagataa caccatcatg catgaatccc
    36841 agaacgggaa agcgtccggc tgtagttcag gcatcgcaac ctgtaatcct aatgcttgca
    36901 tgatcatgac tgccacgcgg gtcaaaaatg caaacacagg attgataaat accgtcatta
    36961 gaccaatcga aacaacgcaa accgccagcc agcttagcgc cttcggcaat ggcaaccagc
    37021 gaattttcaa ctccgcctcg ttctgactgg tttgtcgcga ccacgcatac cagccgtaaa
    37081 tattcgcggc aaagaaaaac acctgtaata acaggctggc atacagctga atctgaaaga
    37141 aaataatgcc aaacaaggtg acgttgatca ggccaaagaa gtagttactg atcttctcca
    37201 gactggcaag gccaatacac agcaacccgg cgatcgtgcc taccgcttcg atccatgaga
    37261 gatcataacc gcctgcccct attggtatat gtaccaggat attctgcaca ctaaaaaaat
    37321 ccatcgttcc cccaaagcgt aatgtgacgc cttattatca attatccacg tagtgtagcc
    37381 gcaaaatcca gcatacgatt gagcggcacc agcgccctct ctcgcagcct ttcatcaaca
    37441 taaacctcgt gattgcttcc ttccagttct aatgcctctg cgatggcctg aaggccattc
    37501 atggccatcc acggacaatg cgcgcagctg cgacaggttg cgccctcacc cgcggttggt
    37561 gcttccagta actctttatc tggcaccgcc tgctgcattt tgtagaaaat gccccgatcg
    37621 gttgccacga taagccgctg atgttgcaat gttttcgcag cagcgatcag ttgactggtg
    37681 gaaccgaccg catccgccat ctctacaata gcttgcggtg attctggatg caccagtatg
    37741 gcagcatccg ggtattcttc ttgcaagcgg gttaacgcct gagtcttaaa ctcatcatgc
    37801 acaatgcagg caccctgcca gcataaaatg tctgcgcccg tctgtttttg cacgtaacgc
    37861 cccagatgtt tgtcaggtgc ccagatgatt ttttcaccca aactatccag atgatcaata
    37921 agttcgacgg caatgcttga agttaccacc caatctgcgc gcgcttttac cgcagcagaa
    37981 gtgttggcgt agacgacgac agtacgatcg ggatgggcat cgcaaaatgc gttaaattct
    38041 tcaacagggc agccgagatc cagtgaacat tcagcctgaa gtgtcggcat cagaattgtt
    38101 ttttccggac tgagaatttt ggcggtttct cccataaatc tcaccccagc gactagcaac
    38161 gtagaagcgg gatgctttgc accgaagcgc gccatttcca gagaatcaga aatacagcca
    38221 ccggtttctt ctgccagttg ttgaatttcg ggatcggtat agtagtgggc aaccatcacc
    38281 gcattacgtt cttttagtag acgttttatc ttctcgcggt aattcgcttt ttcatcaatg
    38341 cttaacggcg tcggcttcgg ggggaaagga taaatcgccg tgtctggatc aaacatcacg
    38401 ctcatcttac catctcgttc tacaggctta acgttaaaac cgacttaagc gtgatattcg
    38461 ctattatcgg cgtaatttgt tttatatact aaacaagata gtcgaatggt ggggaaaagt
    38521 cactcgaatt acgtatgaga tgccgaatgc cacactttgc gtcttattcc tccgacgtag
    38581 agttgtttac gcagcaaacg cggagtatgt catcagggtc aagaattgaa attgagagat
    38641 gcctgaatag caaaaaaacg cctttagggc gcttttttgc attggtgggt cgtgcaggat
    38701 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccct
    38761 tgcgggatta tgctgcttta atcattcagg atggtgggtc gtgcaggatt cgaacctgcg
    38821 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccata cgggtgttac
    38881 ctgaaagatt tctactcgga accaatgttt aaaattggtg ggtcgtgcag gattcgaacc
    38941 tgcgaccaat tgattaaaag tcaactgctc taccaactga gctaacgacc cgagtggtgg
    39001 gtgatgacgg gatcgaaccg ccgaccccct ccttgtaagg gaggtgctct cccagctgag
    39061 ctaatcaccc gatactgcgc tggatactac aaactacttc caccgaaccc accgtaatca
    39121 gtggtgggtc gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc
    39181 aactgagcta acgacccggt ggtgggtgat gacgggatcg aaccgccgac cccctccttg
    39241 taagggaggt gctctcccag ctgagctaat cacccgatac tacgctggat actacaacta
    39301 actatgttag tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
    39361 tgctctacca actgagctaa cgacccactt tttcgttgct ttcggtttgt ttgatatccc
    39421 gtggcaacgg cggcatatat tactgatttc agacttgagc gcaacaaaaa tttcgatgta
    39481 gatcactcaa ctgcttatga ttcgcacgac acgaccagaa ataatgcgac ttctggtcgt
    39541 gtgttatgca ttacatcgcg ttcagacgtt tttgcgcctg tttagcgcca tcagtaccag
    39601 ggtatttact gataacctgc tggtatacgg ctttcgcttt tgcggtgtca cctttgtcct
    39661 gcatgatgac gccgacttta aacatcgcat ctgcagcctt cggtgacttc ggatagtttt
    39721 tcactaccga agcaaaataa tacgccgcat catctttttt acccttgttg tagtttaact
    39781 gacccagcca ataattggcg tttggcaggt aagttgaatc agggtaattt ttgatgaaat
    39841 tctgaaatgc caccattgcg tcatcctggc gggatttatc ctgcaccagc gcaatagctg
    39901 cattgtaatc cgtgtttgca tcaccgcttt tcaccggcgc gccagcattc gaagtaccag
    39961 catcagctgt cggcgtcgtt gatgccacca caccgctttg atcgccgctg gttgattgcg
    40021 ccgctgcacc accgctgctg aggctgtcga tctgcaacag gatctgcttt tgccgctcca
    40081 cgacctgatt cagttgatac tgattttcct gaatctgacc acgcagggaa tcaatatcgg
    40141 attgattatc agagagttgt tgctggagtt gggttaaaag ctggctgtga gcattagaaa
    40201 tacgctcaag ttgagtgacg cggtcttcga ccgagcctga gccgacacta ctgattggtg
    40261 cctgagcaaa agcggcccag ggggccgcta taccaaccag taacgacaga ctcaatagtt
    40321 gatgtctgaa gttactgctc atgcaattct cttagtaaac cagtaccgca cgacggtttt
    40381 tggcatacgc cgcttcgtca tgacccagta ctgcaggttt ttctttaccg taagaaacga
    40441 tggagatctg gtcagcagaa acgcctttac cctgcagata catcttaaca gcgttcgcac
    40501 gacgttcacc cagggagatg ttgtattccg gagtaccacg ttcgtccgcg tgaccttcta
    40561 cggtgacttt gtaagacggg ttgctacgca ggaagtttgc gtgtgcatcc agcatttgag
    40621 cgaagtcaga acggatatcg tacttgtcca gatcgaagta aacgatgttg ttctgctgca
    40681 gctgttgcat ttgcagacga gcctgctctt cggaagacat gttgccgttg ccgccgttcg
    40741 catccatacc agtgccggca cccagcatgc cttcgctgcc gtcattgctg gcgttcttgt
    40801 tggaagaaca tgccgcaatt gccataacag gcagagcaat catcagccct ttcagcactt
    40861 tgttcagttg catttcaatg attcctttac tattcaatta attattatca cagatacggc
    40921 gaccaggcag ggaatttgac ctgtccatca gttgccggaa gacgcgcttt gaaacgccca
    40981 tctgtagaaa ccaaattcag cacggatccc atcccctgag aagagctgta gattaccata
    41041 gtgccgttag gtgccagact tggcgtttca tccaggaacg tggacgacag aacttgtacg
    41101 cctcccgttg ccagatcttg tttggcaatg tgctgctgcc caccgttgga gctgaccatt
    41161 accataaatt taccgtcgct gctgacatcc gcatcctggt tctgcgaacc ttcccaggta
    41221 atacgttgtg gcgcaccgcc gttgatattc actttataaa cctgcggacg accggcctgg
    41281 tcagaagtaa atgccaggtt ctggctgtcc gggaaccagg tcggttcggt attgttactg
    41341 cgaccatcag ttacctggcg gatctgacca gaagccaaat ccattacgta caggttcagg
    41401 ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt ctggcgagaa tgcaggtgca
    41461 ccgttgtgac gcgggaatga agccacctga cgtacagcgc catttgccag cgtctgaata
    41521 accagcgcgg aacgaccgct ttcgaaggtc acataagcca gtttagaacc gtctggtgac
    41581 cacgccggag acatcagcgg ttgcggtgaa cggtgaacga caaactggtt gtaaccgtca
    41641 tagtcagata cgcgcagttc atacgggaac tgaccgccgt tagtctgaac aacgtaggca
    41701 atacgggtac ggaacgcacc tttaataccg gtcagttttt caaatacttc gtcactggcg
    41761 gtatgaccag cataacgcag ccactgcttg ttcactttgt acgagttctg agcaagtaca
    41821 gtacccggtg cgccgccagt gtcaacaagt tgataagcaa cattgtaaga accatccgga
    41881 ttcggagtga cctgaccgac aaccacagcg tcaatgccca gcgcggacca tgcagctggt
    41941 tgtacttcct gcgcactacc cggctgctgt ggcagacgag cacgatctaa cggattaaat
    42001 ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa tatcttcagg tgccgcacca
    42061 ggccccgccc actggaaagg aacaacacca ataggacgac cggaatctac accgctgtcg
    42121 atcacaatgc ggacttcagc atgcagaact gatgcccaca gtatgagaaa accaaatgct
    42181 actcgtaatg cctgcttcat catatctccc ttatctggac cgatggccca cgataattta
    42241 gcagaatgtt aacaaactaa aatacacaaa actaccagaa ccccgtggca accggtgcct
    42301 gatgttgacc gtcggaacag tcaacatcgc gattacggtt tgaagtccaa tggcgcgttt
    42361 ttgaacactt catatactgc ctggcttggt ggtttcggga tcttcgcaag tttagctgct
    42421 gccaacgcag cttgacaaag tgcgggatcg ccaccttcag gtttgatatc cagtaacata
    42481 ccatcgggtg ccagttttat gcgcagcgta caggttttgc ctgcatagga cgatgcgtca
    42541 tagaacttac tttcgatagc agatttaatc tgcccggcat agttattgat atcggcccct
    42601 gatgcgccat tgtttttagt attaccactc ccggcaggcg aagcattgtt ccctttcgcc
    42661 cctccccccg ttttcggtgc attcttacca gagcttagct caccgaaaat atcatctgcc
    42721 tcagcggcag cttttgctgc agcggctttt tcagctgctg cttttgccgc tgccgctttt
    42781 ttgtctgcgg cggctttttc tgccgctgct ttcttatcag ctgctgcctt ttcagcagcc
    42841 gctttttctg ctgctttttt gtcggctgcc gctttctctg ctgccgcttt cttatcagct
    42901 gcagcctttt cagcagccgc tttttctgct gcttttttgt cggctgccgc tttctctgct
    42961 gccgctttct tctcagcttc tgccttggct ttttcagcag cttcggttgc cgctttcttt
    43021 cttgcttcag ctgcagctgc ttctgccgct tcagctttct tcttcagcgc cgcagcagca
    43081 gcctcggctt ttttctgcgc ttcgactgcg gctttggcgg cttctgcttc tgcttttttc
    43141 ttcgcgtctg ccgccgcttt cttcgctgct tcttccgcag ctttagcatc cgcttcggcc
    43201 ttcgctttag catctgccgc cgctttcgct gccgcctctt ccgcttgctt ctgctttaac
    43261 tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc taaccgctct
    43321 ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag ttcttcagca
    43381 gcctgctgct ctttcatctt gcgctgctca tcagaacgct tcgcgcttga ttcctggctt
    43441 tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc gtcgatggac
    43501 gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact ccagatcagc
    43561 gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt gagcttgtcg
    43621 ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg ttacccgctc
    43681 tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac caaccgattt
    43741 cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag gcacatcttt
    43801 tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg acacttccgc
    43861 caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca cggtgtactg
    43921 accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca ccgcctgtga
    43981 ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg ctgtcgccat
    44041 aaagatcagc aacagcacca gcagtacgtc cagcaacggt acaatgttga tttcggactt
    44101 gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt tgctctcgct
    44161 aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt tgtcgtaatt
    44221 cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg ccggaattgc
    44281 ggcgaacaaa ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg caaccatttg
    44341 cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc cccagaccgt
    44401 accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg gaatgtgcgt
    44461 ttccagattt tcaagttcac gattcatgga aatacgcatc gcacgcgacg ccccttccac
    44521 tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt tgaagccgct
    44581 gtaaaagatt tgttccgaac cgctcagatt atcccgtttc ccctggctct cttgatagag
    44641 gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt cgcgcgccgc
    44701 tgcgttaaga atacgggtcc gctggataat aatggcccag gatgcgattg aaaaaccaat
    44761 caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat caaggatatt
    44821 catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg aggcttcatt
    44881 ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcaacaaagt attctcggcg
    44941 ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat ttcagtctgt
    45001 atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat tttacgtacc
    45061 acaaaggcaa cgcgttcagc catcagcgcc tgttgactga aatgatgatg acgcagcatc
    45121 tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac accaccggca
    45181 tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt cactttacat
    45241 cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg cgcgattgag
    45301 gtttgggaat gagggcaagt taagggagcg taaaaattta tgggcctgac tcggcccata
    45361 aatttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca atgagaacaa
    45421 tatcggcaag caatgggcaa aaaaaccctt gccagagaac cttctgcgga cgaaagccca
    45481 cgccgtgaat cacaccagca cagacggccc acataagcag taaaccatgc cagatttcca
    45541 gttcactggt cttcgcggcg aaacgtgatg ggtcccaaaa catacatcct gctaacagaa
    45601 gtgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca tacaaggttg
    45661 cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga cgtgctcaag
    45721 cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa tccatgcgaa
    45781 ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc aatatcttct
    45841 ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag aatgatcggt
    45901 accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga agttgcatcc
    45961 cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg gaacattgcg
    46021 ataccggcag tcaggatgat gcaggccagc gtcagggagg agaaaacaaa cgcccacgcg
    46081 gctttatcca tacgtgcagt caggatggtc agcagcggca gaaccacacc cagtgccgga
    46141 atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc ttcacgaacc
    46201 acttctttat tcagcgggtt agaggctgcg taatggtcca ttgtcgattt cacgacataa
    46261 ccatcgatac cgtacatcac ccatacgcca gccagtgcga agcagaccag cgtcaccagt
    46321 gccgccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt acgcatttgc
    46381 agataggttg cgccctgagt aatgatcatc cctacgctca ccacacctgc cagcaggccg
    46441 aacgggttaa gcagctggaa gaagttaccg gtgtagtaca gacgcagata ttcatcaacg
    46501 ttgaatggta cgccctgcaa caggttaccg aacgctacac caatcaccag cggcggaacg
    46561 aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc ttcaatcttg
    46621 gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagtacgag gatcatcgcc
    46681 acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa cagtgcaccg
    46741 cctgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcgatgga gttaatcata
    46801 attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac ccccatgtcg
    46861 aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca ccagataaaa
    46921 cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg ctgagtagtc
    46981 gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc gaggcgtgca
    47041 aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc gcaaatcagc
    47101 accattgaga agatgagatc gcctgcggtc agtgacgagt tcgccacagc tgtcggcagc
    47161 acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc cgcttctaca
    47221 gcaatccacg gcagcggaat accgtacagc gcggcgcgca gcagccattt tttctcgcca
    47281 atgcggttgc ggatgacact ccagaaagag agcgcaatga ttgccagcag caggaagcca
    47341 cacgccacca tgatacggaa cgcgaagtac agcggcgcta cacgcggaat ggagtctttg
    47401 gttgcctgtt gaatctgagc ttcagtcgca tcagccacgt ttggcgtata gcgtttcagc
    47461 agcagaccgt aaccgagatc tttcttcatg ctattgaact ggtcacgaac ggcctggtca
    47521 gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt acgaatgcgt
    47581 tcttcatgct gcaccatcag ttctttcaga ccgataactg gagtatccac ggaacgcgtt
    47641 gcaatgatgc ccagcgcgta agggatctgg atcgcaaatt tgttcgtctc ctcttcctga
    47701 tcaggaatgc cgaacagagt aaaggcagca ggtgcaggtt gcgtttccca ctcggcttca
    47761 atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc atcacccaga
    47821 acaataacag acagaacggc agccataccg aagctggcag cgatagcaaa ggagcgttta
    47881 gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag gatgaacatc
    47941 gcgccagtca cataaccaga agctacggtg tgaacgaatt tcacctgagc aaccgggtta
    48001 agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt gaaatcggac
    48061 gcgattgggt tttgcatcca gccgtttgca accagaatcc acagtgcgga caggtttgaa
    48121 ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg atcccaaccg
    48181 aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc ttcgattgcc
    48241 agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt agtcccgaac
    48301 tggaactcca tggtcagacc ggtagccaca cccagagcaa agttgatacc aaacaacttg
    48361 ccccagaact tggtcatatc tttataaatc tgtttgccgg aaaggacgta gaccgtttcc
    48421 ataatggcca gcaggaacgc cataccgagc gtcagtggca caaaaaggaa gtggtacatc
    48481 gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat gactccttgc
    48541 tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc atgccccaat
    48601 acaaaattat ttgctcgcct ctatcgttgc catttcatca aaagaagaaa agacaatgat
    48661 aaaaaagtta caaatatgtt aatgaataaa ccaaatttat cccgt
//
LOCUS       DACADF010000015        47399 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.015, whole genome shotgun sequence.
ACCESSION   DACADF010000015 DACADF010000000
VERSION     DACADF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.015"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            148..1365
                     /locus_tag="ISB30_000864"
     CDS             148..1365
                     /locus_tag="ISB30_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP1988526.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1449..2048
                     /gene="yfbR"
                     /locus_tag="ISB30_000865"
     CDS             1449..2048
                     /gene="yfbR"
                     /locus_tag="ISB30_000865"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAP1988527.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2107..3939)
                     /locus_tag="ISB30_000866"
     CDS             complement(2107..3939)
                     /locus_tag="ISB30_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAP1988528.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPI
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4026..4676)
                     /gene="hxpA"
                     /locus_tag="ISB30_000867"
     CDS             complement(4026..4676)
                     /gene="hxpA"
                     /locus_tag="ISB30_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAP1988529.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4687..5181)
                     /locus_tag="ISB30_000868"
     CDS             complement(4687..5181)
                     /locus_tag="ISB30_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP1988530.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5264..5719)
                     /locus_tag="ISB30_000869"
     CDS             complement(5264..5719)
                     /locus_tag="ISB30_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAP1988531.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6057..7259
                     /gene="ackA"
                     /locus_tag="ISB30_000870"
     CDS             6057..7259
                     /gene="ackA"
                     /locus_tag="ISB30_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP1988532.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7334..9478
                     /gene="pta"
                     /locus_tag="ISB30_000871"
     CDS             7334..9478
                     /gene="pta"
                     /locus_tag="ISB30_000871"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP1988533.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9668..11188
                     /gene="yfcC"
                     /locus_tag="ISB30_000872"
     CDS             9668..11188
                     /gene="yfcC"
                     /locus_tag="ISB30_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP1988534.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11221..11763)
                     /gene="yfcD"
                     /locus_tag="ISB30_000873"
     CDS             complement(11221..11763)
                     /gene="yfcD"
                     /locus_tag="ISB30_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAP1988535.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11821..12372)
                     /gene="yfcE"
                     /locus_tag="ISB30_000874"
     CDS             complement(11821..12372)
                     /gene="yfcE"
                     /locus_tag="ISB30_000874"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAP1988536.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12428..13072)
                     /gene="yfcF"
                     /locus_tag="ISB30_000875"
     CDS             complement(12428..13072)
                     /gene="yfcF"
                     /locus_tag="ISB30_000875"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAP1988537.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13208..13855
                     /gene="yfcG"
                     /locus_tag="ISB30_000876"
     CDS             13208..13855
                     /gene="yfcG"
                     /locus_tag="ISB30_000876"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAP1988538.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLNHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            13912..14274
                     /gene="folX"
                     /locus_tag="ISB30_000877"
     CDS             13912..14274
                     /gene="folX"
                     /locus_tag="ISB30_000877"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAP1988539.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYTDSVSMTLSWQR"
     gene            14295..15188
                     /locus_tag="ISB30_000878"
     CDS             14295..15188
                     /locus_tag="ISB30_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAP1988540.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHERKERLCQSRWNTTQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRFGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPTILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15347..16120)
                     /gene="hisP"
                     /locus_tag="ISB30_000879"
     CDS             complement(15347..16120)
                     /gene="hisP"
                     /locus_tag="ISB30_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAP1988541.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSDGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(16128..16844)
                     /locus_tag="ISB30_000880"
     CDS             complement(16128..16844)
                     /locus_tag="ISB30_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1988542.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(16841..17527)
                     /locus_tag="ISB30_000881"
     CDS             complement(16841..17527)
                     /locus_tag="ISB30_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311219.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP1988543.1"
                     /translation="MLYGFSSVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(17617..18399)
                     /gene="hisJ"
                     /locus_tag="ISB30_000882"
     CDS             complement(17617..18399)
                     /gene="hisJ"
                     /locus_tag="ISB30_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAP1988544.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(18620..19402)
                     /gene="argT"
                     /locus_tag="ISB30_000883"
     CDS             complement(18620..19402)
                     /gene="argT"
                     /locus_tag="ISB30_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAP1988545.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(19668..20237)
                     /locus_tag="ISB30_000884"
     CDS             complement(19668..20237)
                     /locus_tag="ISB30_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP1988546.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            complement(20332..21849)
                     /gene="purF"
                     /locus_tag="ISB30_000885"
     CDS             complement(20332..21849)
                     /gene="purF"
                     /locus_tag="ISB30_000885"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP1988547.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVVMIIGHGMVAFRDPNGIRPLVLGKRDIDDNRTEYMVASESVALDTLG
                     FEFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(21886..22374)
                     /gene="cvpA"
                     /locus_tag="ISB30_000886"
     CDS             complement(21886..22374)
                     /gene="cvpA"
                     /locus_tag="ISB30_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAP1988548.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(22815..23477)
                     /gene="dedD"
                     /locus_tag="ISB30_000887"
     CDS             complement(22815..23477)
                     /gene="dedD"
                     /locus_tag="ISB30_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAP1988549.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVESPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(23467..24735)
                     /gene="folC"
                     /locus_tag="ISB30_000888"
     CDS             complement(23467..24735)
                     /gene="folC"
                     /locus_tag="ISB30_000888"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP1988550.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVGQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(24805..25719)
                     /gene="accD"
                     /locus_tag="ISB30_000889"
     CDS             complement(24805..25719)
                     /gene="accD"
                     /locus_tag="ISB30_000889"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAP1988551.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(25875..26534)
                     /locus_tag="ISB30_000890"
     CDS             complement(25875..26534)
                     /locus_tag="ISB30_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP1988552.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(26617..27429)
                     /gene="truA"
                     /locus_tag="ISB30_000891"
     CDS             complement(26617..27429)
                     /gene="truA"
                     /locus_tag="ISB30_000891"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP1988553.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(27429..28442)
                     /locus_tag="ISB30_000892"
     CDS             complement(27429..28442)
                     /locus_tag="ISB30_000892"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP1988554.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(28508..29644)
                     /gene="pdxB"
                     /locus_tag="ISB30_000893"
     CDS             complement(28508..29644)
                     /gene="pdxB"
                     /locus_tag="ISB30_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAP1988555.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGITTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     LIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            29743..30738
                     /locus_tag="ISB30_000894"
     CDS             29743..30738
                     /locus_tag="ISB30_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAP1988556.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(30735..31913)
                     /locus_tag="ISB30_000895"
     CDS             complement(30735..31913)
                     /locus_tag="ISB30_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HAP1988557.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(32178..33398)
                     /gene="fabB"
                     /locus_tag="ISB30_000896"
     CDS             complement(32178..33398)
                     /gene="fabB"
                     /locus_tag="ISB30_000896"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAP1988558.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            33557..35563
                     /gene="mnmC"
                     /locus_tag="ISB30_000897"
     CDS             33557..35563
                     /gene="mnmC"
                     /locus_tag="ISB30_000897"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAP1988559.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAYPQAQLQRLHFISFEK
                     FPLARADLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDEGRITLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMELTLPLPCSTPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPTELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIHYQYQLQDLSRKDDGWLLNFAGDQ
                     QATHSLVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSDEDQQQNRQRLIDCFPQVQWAKEVDVSGKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(35684..35962)
                     /locus_tag="ISB30_000898"
     CDS             complement(35684..35962)
                     /locus_tag="ISB30_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAP1988560.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(35996..36544)
                     /locus_tag="ISB30_000899"
     CDS             complement(35996..36544)
                     /locus_tag="ISB30_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAP1988561.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(36544..37353)
                     /locus_tag="ISB30_000900"
     CDS             complement(36544..37353)
                     /locus_tag="ISB30_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAP1988562.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(37353..38177)
                     /gene="mepA"
                     /locus_tag="ISB30_000901"
     CDS             complement(37353..38177)
                     /gene="mepA"
                     /locus_tag="ISB30_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAP1988563.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IIGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(38181..39266)
                     /gene="aroC"
                     /locus_tag="ISB30_000902"
     CDS             complement(38181..39266)
                     /gene="aroC"
                     /locus_tag="ISB30_000902"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP1988564.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTEIPRW"
     gene            complement(39301..40233)
                     /gene="prmB"
                     /locus_tag="ISB30_000903"
     CDS             complement(39301..40233)
                     /gene="prmB"
                     /locus_tag="ISB30_000903"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP1988565.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNRAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            40399..40950
                     /gene="smrB"
                     /locus_tag="ISB30_000904"
     CDS             40399..40950
                     /gene="smrB"
                     /locus_tag="ISB30_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAP1988566.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41010..41834)
                     /locus_tag="ISB30_000905"
     CDS             complement(41010..41834)
                     /locus_tag="ISB30_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAP1988567.1"
                     /translation="MKKMSGLFFLLLTGLLSATAWADVTFRDVKTYTGFWGNGATTKV
                     VFRIDVLTPEGVYYGEYAQDGYTLWLGNIPNTVWTGPGSAPTMNITHQSNTVDNSNCP
                     GITDGWSCVDLGVDIIVHGETHGCPWLVSTNTVSTAARGYTYIGPSAKQTRCPTEPVG
                     PYDISWNENYVLHNKMLTLQSTGGVITQTLSTYLMRDGRVCDGSRMDDRGAYCRFMSY
                     MITFSTSGCDDSKVTVTPVAHPITDKQLHDMQIRIDTSSQQPIDATCRFQYILNEL"
     gene            complement(41836..42363)
                     /locus_tag="ISB30_000906"
     CDS             complement(41836..42363)
                     /locus_tag="ISB30_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1988568.1"
                     /translation="MSLKRIFHCAAALAAVLLTPNVQAADNLHFYGNLLSKSCTLVVQ
                     GEVLAEVHFPTVSRRDLMVTGQSARVPVVFQLKDCKGPAQYEVRVTLTGTEDSEQPGF
                     LALDAASTAQGVGIGMEKTDGTAVLINNTSGATFVLSSGNNDLHFNAWLQAKSGRDVT
                     MGDFTASLTATFEYI"
     gene            complement(42360..42839)
                     /locus_tag="ISB30_000907"
     CDS             complement(42360..42839)
                     /locus_tag="ISB30_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1988569.1"
                     /translation="MKRFALCLFGCYCGIAQAADSDITFHGTLVSPPACAISDGKTVE
                     VEFRDVIIDNINGNNFRQNVPYTITCDPDVRDDAWEMTLSWTGSQTSYDNAAIETNVT
                     GLGIELQQNGQPFTLGTPLKINLSTPPTLQAVPVKASDASLSEGTFSAWATLQVDYQ"
     gene            complement(42836..43327)
                     /locus_tag="ISB30_000908"
     CDS             complement(42836..43327)
                     /locus_tag="ISB30_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1988570.1"
                     /translation="MKGVLLPVAGMVLLACGSAWADSKTVNLTLRILVDAPPPCTVKG
                     SAVEFGNVIIKNIDGSNYRQPVGYTLNCSNSVSDDLQMQLQGTTTTINGETVLSTGIN
                     GFGIRIQDASSHAPVALAGNNWLPFNVNSRPGFEAVPVKQSGTQLTASEFSATMTMVV
                     EYQ"
     gene            complement(43344..44096)
                     /locus_tag="ISB30_000909"
     CDS             complement(43344..44096)
                     /locus_tag="ISB30_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP1988571.1"
                     /translation="MFYKLSCSMVVSTTLAVLLTTTNLAAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKITSPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKSGKANTLQIALQTRIKLFWRPKALEKIDMRKPWQFKV
                     TLTRSGQDYIVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKMNSAPV
                     LTYINDYGARLPLVFRCEGDTCKVDEAQSLKG"
     gene            complement(44116..46764)
                     /locus_tag="ISB30_000910"
     CDS             complement(44116..46764)
                     /locus_tag="ISB30_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP1988572.1"
                     /translation="MPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKG
                     DTKIDLGKFSKKGYVDAGKYNLRVFINKQSLSDEYDINWYVSENDPTKTYACLTPELV
                     AALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWD
                     PPSRWDDGISGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDY
                     QHTRSNDDEDDSSNSTTSKNWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIG
                     SSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDS
                     VSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSG
                     GEASWGVADGWSLYGGALADEHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYG
                     KGKLDGNSFRVSYAKDFDELNSRVTFAGYRFSEKNFMTMSEYLDASQSDMARTGNDKE
                     MYTITYNQNFAAAGVSVYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIRNMSISVTGY
                     RYEYDDNTDKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFNRIDDATHYQINVG
                     TSEQHGSVDGYLSHDGTLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEA
                     TQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVDD
                     EGNVYLAGINAGEHMMVFWEGSAQCEIVLPKPLPADLFRGLLLPCEQKGTTAPDSLAP
                     EIKPVIQDQTRQVTPSEAPTSISATQ"
     gene            complement(46845..>47399)
                     /locus_tag="ISB30_000911"
     CDS             complement(46845..>47399)
                     /locus_tag="ISB30_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1988573.1"
                     /translation="AKTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCSIVPD
                     DHKLEVDMGDIGAAKLTGGGTTTPKDFQIHLQDCVFTTESNMETTFTGTNYAGPANAD
                     NYALFNVDSGVAMNHVSLVIGDTHGKGYKVGEKIVQPIVMDASTSKGKDKQTLNFKAW
                     LVGETDAPDLGQFETLTTFQITYL"
BASE COUNT        11455 a        12922 c        11917 g        11105 t
ORIGIN      
        1 attaagttat taacagcaca atcgagcctt cccctctggc aaaatcttat tctgcagacc
       61 ttcaaaacac cgtcctgggg gagtacattg ttctaagctg acttccacgg cagggagtgg
      121 cgataacagc aaaaaaggtc aagattcatg tcccccattg aaaaatccag caaattagag
      181 aatgtctgtt atgacatccg tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa
      241 ggtaacaagg ttctgaaact gaacatcggc aaccctgccc cgttcggttt tgacgcgcca
      301 gatgaaattc tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc
      361 aaaggtcttt actccgcgcg taaagccatc atgcagcact accaggctcg tggcatgcgt
      421 gatgttaccg tggaagatat ttacatcggc aatggtgtat cggagcttat cgttcaggca
      481 atgcaagcat tgctgaacag cggggacgaa atgttggttc ctgcacctga ttacccacta
      541 tggaccgcgg cggtttcact ctccagcggt aaagcggtgc attatctttg cgatgaatcc
      601 tctgactggt tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg
      661 atcgttatta tcaacccaaa taacccaacc ggcgcggtat attccaaaga gcttttaatg
      721 gagattgtgg agattgcacg tcagcataat ctcattatct ttgccgatga aatttatgac
      781 aaaattctct acgacgacgc tgagcatcac tccattgcgc cgcttgcacc tgacctgctg
      841 accattacct ttaacggact gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg
      901 atggtgttga acgggccgaa aaaacacgct aaaggttaca tcgaaggtct ggaaatgctg
      961 gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg ccattcagac cgcactgggt
     1021 ggttatcaga gcatcagtga atttattacc cctggcggtc gtctttatga gcagcgtaac
     1081 cgcgcgtggg aattgatcaa cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg
     1141 ctgtatatgt tcccgaaaat cgacgccaaa cgctttaaca ttcacgacga tcagaaaatg
     1201 gtgctggatt tcctgttgca ggaaaaagtc ctgttggtgc aagggacggc attcaactgg
     1261 ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct
     1321 ttgagtaagt tcgcgcgttt cctttctggt tatcatcagc tgtaatctta atttcactgc
     1381 cggagattgc atccggcagt gttatcccgc cacaatgacc tgatgatgtc atcatacgta
     1441 aggtcactat gaaacagagc catttttttg cccatctctc ccgcctgaaa ctcattaacc
     1501 gctggccgct aatgcgcaac gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag
     1561 cgatggtcgc ccatgcgctg gcagctatca aaaatcgtaa atttggcggt aatgtcaacg
     1621 ccgaacgtat cgctttactg gcgatgtacc acgatgcttc agaagtgctc accggagatc
     1681 tccctactcc ggtgaaatac ttcaattcgc aaatcgctca ggaatacaag gctattgaaa
     1741 aaatcgctca gcaaaaactg gtcgatatgg ttccggaaga gctacaggat atctttgcgc
     1801 cgttaattga cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa caggcagatg
     1861 cactgtgtgc atatctgaaa tgtctggaag aactcgcggc cggaaataat gaattcttgc
     1921 tggcaaaaac gcgactggaa gcgacgcttg aagcgcgtcg cagccaggag atggactact
     1981 tcatggaagt atttgttccc agcttccatc tttcgctcga tgagattagc caggattcac
     2041 cgctgtaagc agctggagtc tgcgtcgcat caggcaataa gcgccggatg cgacatcagg
     2101 ctcttgtcaa aacggaaaca gcatcgggat catcaccaca caaaccgcca tcacgataat
     2161 ggtgaacggt acccccaact taacaaagtc actgaagctg taattccccg gacctaaaac
     2221 cagtgtgtta acaggtgaag atatcggtgt cataaaggcg gcggatgctg ccatcgccac
     2281 gaccatcgca aatggataag gcgacacccc catcgttttg gcagcagcca gcgcaatcgg
     2341 agccatcaac accgccgtcg cagtattaga aataaatagc ccaataaccg ccgacaagac
     2401 aaacaaacaa cccagcatca tatgtggccc ataaccgccg ccaatgtcca tcagcccttt
     2461 caccgccagc gcgacacctc ccgttttctg taatgccaca gcaaacggca tcatcccaac
     2521 gatcaaaata atgctcggcc agtgaatgga tttataggca ctttcggcat cgatacagcg
     2581 gaatttcccc atcagcaggc aggcgatgat agcggcaaca ggattaggaa tttcatcggt
     2641 cagcattaac gccaccatta gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc
     2701 gggcgatgct tcactcacct cttccggtaa gttcagcgcc acgaagtcgc ggccctgttt
     2761 ggccagcata ccgatcagtt tccagttacc aacaaccaga atgatatcgc ccagcagcag
     2821 aggctcatcc gccagcgaac cttccagcgc cacaccattg cgctttagcc ccaccacatt
     2881 caatccgtag cgggtacgaa aaccaatttc gcgcaccgat ttaccaataa gttctgactc
     2941 aggaattaat gaaatctctg ccatgcccac atcaagggcc tggtcagaaa aatactcgcc
     3001 gcgcagtacc atcggctcca gcaattgctc actacaaaat tgccggagat cgacatcagc
     3061 cgcagacata tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt
     3121 cacgataacg cgacgaaaac gccgccagcg ttcaacaccg atgacgttag caccataacg
     3181 ttcacgtaat ttgagatcgt ccagtcgttg accaatcatt ggcgatccgg ggcgaatagc
     3241 cagacgtcgc gcacgcccgg tcagtcgata ttcacggata agatcgcgaa aggttcggcg
     3301 cgtccagcct tcgcgttgtg gggtctgggt atcccctttc agcatgaaac gcatcactaa
     3361 catatacaaa atacccagca ccagcacgac caggccaata ggtgttacgc taaagaaact
     3421 gaagccgtga tagccttcac gcagtaattc actgttgact accaggttcg gcggcgtcgc
     3481 caccagcgtc atcatgccgc ttatcagccc ggcaaaactt aacggcatca tcagacgcga
     3541 cggcgacgtt tgcatacgca tggcaacgct taacaccacg gggataaaga tagcgacaac
     3601 gccggttgaa ctcataaacg cgccaagtcc cgcgacggtc agcatcaaca aaaccaacat
     3661 tttgatttca ctgttgcccg ccactttgac cagccatgtc cccattacag tggcaacacc
     3721 ggtacggacc aaaccatcgc caataatgaa caaggcggca atcaggacaa cgttaggatc
     3781 ggaaaagccg gaaaatactt cggggaccgt cagcgttccg cttaatgcaa acgcgacaat
     3841 aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac
     3901 ggccagtaat gaaagaaccc aaatcaattc accgttcaca acttatcctt gttaattgag
     3961 ggggataact tgattctgcc ataaaaaagc cccgacgaga cggggctaaa tcatgatcag
     4021 gtgtttcact gaataataac atcgccattt ggctgtttgg tcacagtaat ttgctccaga
     4081 ctgtggagga ctaaatcgac ttcattcagg cgcggggtat ctgccggggc attaaccgca
     4141 ataacatggc aacccgccgc cagacctgaa agcacgccag cgggagcatc ttccaccaca
     4201 acacactcct gcggcgcaag ccccagcagc tgcgcgccta acagatacgc atcaggttct
     4261 ggttttccgc gcttcacccg ctcagcggtt acaaacacct ctggcgcggg aagccccgct
     4321 attttatgac gcgctcgcgc taccggcatg gagccagaag tcacaatagc ccacggaata
     4381 cctgctttat tcaaatgatt gagtaaggcg attgcccccg gaagcgcggt aataccttcg
     4441 gtttccgtgg cctcgatgtg ttccagacgc gtaaactcgg cggcaatgtc tgcctcggat
     4501 ttgcccgcca taaaatggcg cagagaggtg atcgcctgtt taccgtgaat gaaagccagc
     4561 acctcttccg gcgataaccc atgacgtctg gcccagttgc tccacgcccg ttctaccgca
     4621 ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg
     4681 gcctcctcag gcgttaataa tttgattaat ttcgttggcg ctcaaatggt actgacgcgg
     4741 gcaggcatgc cacacattaa gcatgcgctg atatttttcc cacattggcg tctgggcgtt
     4801 aaaaccgtga gttccagcgt caaaatgggt atagcgccct tccacattaa ccataaagcg
     4861 gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg
     4921 ttcatcgatg gattgctggt cctgcaaatt agaccaggaa acatgcaacg catgatacat
     4981 ctccataatg tcgatgatag tgcggcaggt ttcttctttc agctcgccaa actcgcgatc
     5041 cagttcacgc atctgtaatc cataaccacg ctcaatgatt gtttgcaggc gacggtaacg
     5101 ttcagcattt gccggatcga gcatagtcat catcttgtac tggttagaca aaataagacg
     5161 ttgcgcgttg gtcatttcca ttattgactc ctgtatcact ctactacggt gaaaaaaaag
     5221 aaggctgagt atgccttctt ttatatgcgt aatcaggggt caattacaaa tcatcaagga
     5281 aagttttatc cagttgtttg aaggcacgct taagcgtgtc agctaatgcc tggtaatcag
     5341 gcttgccttc aacgggtgcc aacacctgtc cagactcctg caatttaccg cgaacttcat
     5401 aaaaccagtt gaggattgca ggaggtaatg gcgttacaga acgcttgccc agccaccaca
     5461 atccctgcat gggtaaactt aaggcgaaca gcgcagtggc aactgccggc ccaagctgac
     5521 cgcccagggc aatctgccag cagagagtaa atacggcgat cggcggcata aaacggatcg
     5581 cataacgcgt catcttgata acgcgatttt cgacaaagac tggggcaagg cgtttttcca
     5641 gcggccacgt ctttgagtaa tgctgtcccc ggcgaaacaa gctaaaaaaa ttaacagaac
     5701 gattatccgg cgttgacatg cttcacctca acttcacata taaagattca aaaatttgtg
     5761 caaattcaca actcagcggg acaacgttca aaacattttg tcttccatac ccactatcag
     5821 gtatccttta gcagcctgaa ggcctaagta gtacatattc attgagtcgt caaattcata
     5881 tacattatgc cattggctga aaattacgca aaatggcata gactcaagat atttcttcca
     5941 tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc
     6001 tacgctctat ggctccctga cgttttttta gccacgtatc aattataggt acttccatgt
     6061 cgagtaagtt agtactggtt ctgaactgcg gtagttcttc actgaaattt gccatcatcg
     6121 atgcagtaaa tggtgaagag tacctttctg gtttagccga atgtttccac ctgcccgaag
     6181 cacgtatcaa atggaaaatg gacggcaata aacaggaagc ggctttaggt gcaggcgccg
     6241 ctcacagcga agcgctcaac tttatcgtta atactattct ggcacaaaaa ccagaactgt
     6301 ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg cggcgaaaag tataccagct
     6361 ccgtagtgat tgatgagtct gttattcagg gtatcaaaga tgcagcttct tttgcaccgc
     6421 tgcacaaccc ggctcacctg atcggtatcg aagaagctct gaaatctttc ccacagctga
     6481 aagacaaaaa cgttgctgta tttgacaccg cattccacca gactatgccg gaagagtctt
     6541 atctctacgc cctgccgtac aacctgtaca aagagcacgg catccgtcgt tacggcgcgc
     6601 acggcaccag ccacttctat gtaacccagg aagcggcaaa aatgctgaac aaaccggtag
     6661 aagaactgaa catcatcacc tgccacctgg gcaacggtgg ttccgtttct gctatccgca
     6721 acggtaaatg cgttgacacc tctatgggcc tgaccccgct ggaaggtctg gtcatgggta
     6781 cccgttctgg tgatatcgat ccggcgatca tcttccacct gcacgacacc ctgggcatga
     6841 gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg cctgctgggt ctgaccgaag
     6901 tgaccagcga ctgccgctat gttgaagaca actacgcgac gaaagaagac gcgaagcgcg
     6961 caatggacgt ttactgccac cgcctggcca aatacatcgg tgcctacact gcgctgatgg
     7021 atggtcgtct ggacgctgtt gtattcaccg gtggtatcgg tgaaaatgcc gcgatggttc
     7081 gtgaactgtc tctgggcaaa ctgggcgtgc tgggctttga agttgatcat gaacgcaacc
     7141 tggctgcacg tttcggcaaa tctggtttca tcaacaaaga aggtacccgt cctgcggtgg
     7201 ttatcccaac caacgaagaa ctggttatcg cgcaagacgc gagccgcctg actgcctgat
     7261 ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga
     7321 aagaggataa accgtgtccc gtattattat gctgatccct accggaacca gcgtcggtct
     7381 gaccagcgtc agccttggcg tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt
     7441 tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat
     7501 cgtgcgtgcg aactcttcca ccacgacggc cgctgaaccg ctgaaaatga gctacgttga
     7561 aggtctgctt tccagcaatc agaaagatgt gctgatggaa gagatcatcg cgaactacca
     7621 cgctaacacc aaagacgctg aagtcgttct ggtggaaggt ctggtcccga cacgtaagca
     7681 ccagtttgcc cagtctctga actacgaaat cgccaaaacg ctgaacgcag aaatcgtctt
     7741 cgttatgtct cagggcactg atactccgga acagttgaaa gagcgtatcg aactgactcg
     7801 caacagcttc ggcggtgcaa aaaacaccaa tattaccggc gttatcgtta acaaactgaa
     7861 cgctccggtt gatgagcagg gtcgtacccg tccggatctg tccgagattt ttgacgactc
     7921 caccaaagca aaagtgaaca acgttgatcc ggcgaagctg caagaatcca gcccgctgcc
     7981 ggttctcggc gctgtgccgt ggagctttga cctgatcgcg actcgtgcga tcgatatggc
     8041 tcgccacctg aatgcgacca tcatcaacga aggcgacatc aatactcgcc gcgttaaatc
     8101 cgtcactttc tgcgcacgca gcattccgca catgctggag cacttccgtg ccggttctct
     8161 gctggtgact tccgcagacc gccctgacgt gctggttgcc gcttgcctgg ctgccatgaa
     8221 cggcgtagaa atcggtgccc tgctgctgac tggcggctac gaaatggacg cgcgcatttc
     8281 taaactgtgc gaacgtgctt tcgctaccgg cctgccggta tttatggtga acaccaacac
     8341 ctggcagact tctctgagcc tgcagagctt caacctggaa gttccggttg acgatcacga
     8401 gcgtatcgag aaagttcagg aatacgttgc taactacatc aacgctgact ggatcgattc
     8461 tctgactgcc acttctgagc gcagccgtcg tctgtctccg ccagcgttcc gttatcagct
     8521 gactgaactt gcgcgcaaag cgggcaaacg tatcgttctg ccggaaggtg acgaaccgcg
     8581 taccgttaaa gcagccgcta tctgtgctga acgtggtatc gcaacttgcg tactgctggg
     8641 taatccggca gagatcaacc gtgttgcagc ctctcagggt gtagaactgg gtgcaggcat
     8701 tgaaatcgtt gatccagaag tggttcgcga aaactatgtt ggtcgtctgg tcgaactgcg
     8761 taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag ctggaagaca acgtggttct
     8821 cggtacgctg atgctggaac aagatgaagt tgatggtctg gtttccggtg ctgttcacac
     8881 caccgcaaac accatccgtc cgccgctgca gctgatcaaa actgcaccgg gcagctccct
     8941 ggtatcttcc gtgttcttca tgctgttgcc ggaacaggtt tacgtttacg gtgactgtgc
     9001 gatcaacccg gatccgaccg cagaacagct ggcagaaatc gcgattcagt ccgctgattc
     9061 cgctgcggcc ttcggtatcg aaccgcgcgt tgctatgctc tcctactcca ccggtacttc
     9121 tggtgctggt agcgacgtag aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg
     9181 tcctgatctg atgatcgacg gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc
     9241 gaaatccaaa gcaccgaact ctccggttgc aggtcgcgct accgtgttca tcttcccgga
     9301 tctgaacacc ggtaacacca cctacaaagc ggtacagcgt tctgctgacc tgatctctat
     9361 cggaccgatg ctgcagggca tgcgcaagcc ggttaacgac ctgtcccgtg gcgcactggt
     9421 tgatgatatc gtctacacca tcgcgctgac tgcaattcag tctgcacagc agcagtaatc
     9481 tcgtcatcat ccgcagcttt gcgctgcgga tatctgaacc ggaaatagtc actatttccg
     9541 gttttttatt ctcttaattt gcattaatcc tttctgatta tcttgcttaa ctgcgctgca
     9601 tcaatgaatt gcgccatctc actttgcata cttaccactt tgttttgtgc aagggaatat
     9661 ttgcgctatg tccgcaatca ctgaatctaa accaacaaga agatgggcaa tgcccgatac
     9721 gttggtgatt atcttttttg ttgccatttt aaccagcctt gccacctggg tagttccggt
     9781 gggcatgttt gacagtcagg aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa
     9841 agtcgtagat ccacactcat ttcgcattct gactaacgaa gcaggcgaac ctgagtatca
     9901 ccgcgtacag ctgttcacga cgggcgatga acgcccgggt ctgatgaact tcccgtttga
     9961 agggttaacc tcaggatcga aatacgggac agccgttggc atcatcatgt ttatgctggt
    10021 gattggcggc gcgtttggca ttgtgatgcg tacaggaacc attgataacg gtatcctggc
    10081 gcttattcgc catacccgcg gaaatgaaat tctctttatt cctgcgctgt ttattctgtt
    10141 ttcacttggc ggcgcaatat ttggtatggg agaagaggcc gtcgcctttg ccattatcat
    10201 cgcaccgcta atggtccggc tgggctatga cagtattacc accgtccttg tgacctatat
    10261 tgccacgcaa atcggttttg ccagttcgtg gatgaacccg ttttgtgtgg ttgttgctca
    10321 gggtattgcc ggcgttccgg tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat
    10381 cgccactctg attggcctga tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc
    10441 tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt gaaaagcagg ctgatgttga
    10501 acaacgtccg tttacctttg gtgactggct ggtattgatt gtcctgaccg ccgtaatggt
    10561 ctgggttatt tggggcgtga tcgttaatgc ctggtttatt cctgaaattg ccagccagtt
    10621 cttcaccatg ggtctggtga ttggcatcat cggcgttgtt ttccgcctta acggcatgac
    10681 ggttaatacc atggcttcat cctttaccga aggggcgcga atgatgatcg cccctgccct
    10741 gctggtgggt ttcgccaaag ggattttgct gctggtcggt aatggtgaag cgggtgatgc
    10801 cagcgtgtta aataccatcc tcaacagcat tgccaatgcc attagcggtc tggataacgc
    10861 agtcgcggcc tggtttatgt tgctcttcca ggcggtattt aatttcttcg tgacgtccgg
    10921 ttctggtcag gcggcgttaa ccatgccgtt actggcaccg cttggcgatc tggtcggtgt
    10981 taaccgtcag gttaccgtgc tggcgttcca gtttggtgat ggcttcagcc acatcattta
    11041 cccaacctca gcttcgttaa tggcgacgct cggtgtttgc cgggtggact tccgtaactg
    11101 gctgaaggtg ggtgcgaccc tgcttggact gctgtttatt atgtccagcg tcgtggtgat
    11161 cggcgctcag ttgatgggct accactaaaa atgttaagag ccgcgattgc ggctcttttt
    11221 tcattctgcc gtttcagtct cgacggcttc atttttggca ttgcgcttca tccacaacgc
    11281 cagcgctttc agcgagtctg gagtgaactc atcgcagcgt gcggtgattt cttccggcgt
    11341 cagccagcaa acttcactga cttcatcttc ctgtagagcg aagggaccgt gagagacgca
    11401 gctgaacaac gcgccccaga cacggcaatt tttatcttcg aaatagaact gcccgtgctc
    11461 ggcaaagggg acaccggcaa tgcccaactc ttcttccgct tcgcgacgcg cggattccag
    11521 cagctgctca tcggcctgga ctacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa
    11581 gtcttttgtc tcggtacgac gctggaccag aattttgccc atgccatcat gcacgacgat
    11641 ataagttgca cgatgacgca gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat
    11701 gacttcgttc tcttcattga caatatccac ccattcagta cttgccaaac gacgctgttc
    11761 cattatcagg aaaccttctt tttctggcgc tcttacggcg cattttgagt tgtgggtaaa
    11821 ttacggatta atcgcgacct gcgcaatgat actttgatca ttgagtgcga ttacactaag
    11881 tacgtcatta tccagcatgc cataactcgc cggattaccg cctttcggaa tactcaccga
    11941 gccggggttg aagtggaaaa tctccccccg ctgttctgcc actggcagat gggtatgacc
    12001 gtacaccagc acatcgttct ggtttaaagc aggtagattt tccgggccaa aaagatgacc
    12061 atgcgtcaaa aacagacgtt gtttttccag taatacctgt tgccacggcg cggttatcgg
    12121 gaaatgcagc agcatttgat ccacttcgct gtcgcagttg ccgcgcacag cgataacctt
    12181 atgtgccact tcattaagcc gttcagcgac tttggctggc gcgtaaccct ccggtaaagc
    12241 attacgcggg ccatgattca acacgtcgcc aaggatcacc agccactggg caccgctttg
    12301 ggcaaacaac tccagaacac gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa
    12361 catcagtttc atcactcact cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc
    12421 gttgctatca gccagattgc ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct
    12481 gccactggaa tgtcgcataa tccaccaggc gttccggcac ctcatcgcca tgtagtacca
    12541 ggcggttaat cattagcgcc agatcagtat cagcaatgca ccattcacca aataaattcg
    12601 gctgacccag tgctaacaaa tgctctgcca tcgcgaacag tttctctgca ctggcttttc
    12661 cctcggccgt tagtggcgct tttttcgccc ccgcaaagac aacatccgtc gggcgttctt
    12721 cgcggatggg catcagatcg ctgcgcagcc aggcctgaat ctgtcgtgca cgcgcacgat
    12781 tttctaaatc aagcggataa atacgttccc aggtcggtgg cgcaaatcga tcttccagat
    12841 actccgcaat ggcagaagat tcactcaact caaaatcatc gatttgtaat aacggcacac
    12901 ggcgtgtctg accgtaacct tgccacgtcg gctgcaaatg ttcaccgctg tcgaggtcga
    12961 tggtcttgat atgaaacgac aggcctttct cctgcaacgc cacccaggcg gataacacat
    13021 aaggggagaa aaaatgggca tctgaccaaa gcgtaatagc gggtttactc attatgtcct
    13081 cggcaataaa gggggcttgc tatcaaacta tagagccttt ttgtcactgt cacgtgacga
    13141 atactcacgc aagtcgccgc gctttctata cttgtggtgt tcagatgatt agacaaccgg
    13201 agctgcaatg atcgatctct atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt
    13261 tctcgaagaa gcagggctgg attatcgctt gataaaggta gacctgggga aaggaggtca
    13321 gtttcgcccg gaatttttgc gcatttcgcc taacaacaaa attccggcaa ttgttgatca
    13381 ttctccagcc gatggcggcg aaccgctaag cctctttgag tctggtgcca ttttgttgta
    13441 tctggctgag aaaacgggac tctttttgaa tcatgaaacg cgtgaacgcg ccgtcacatt
    13501 acagtggtta ttctggcagg taggcggact ggggccgatg cttgggcaaa atcatcattt
    13561 taatcacgca gccccccaaa ccattcctta cgctattgaa cgttatcagg ttgaaactca
    13621 gcgtctgtac catgtactga acaagcggct ggaaaattcg ccctggctgg gaggcgagaa
    13681 ctacagcatt gcggatattg cctgctggcc gtgggttaat gcctggactc gccagcgaat
    13741 tgacctcgca atgtatccgg cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc
    13801 caccgggcag gcactgctaa aagcacaaca cggtgatgag cgttcggata gttaacagaa
    13861 acaggttctc gtgtattatt tcattctaag taaaacaacg gagaacctgc aatggcacaa
    13921 cctgccgcta ttattcgtat aaagaacctt cgtttacgta cgtttatcgg aattaaggaa
    13981 gaagaaatta acaaccgtca ggatattgtt atcaatgtga cgatccacta ccccgccgat
    14041 aaagcgcgca ctagcgaaga tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac
    14101 attattcagc atgtagagaa taaccgtttc tctttgctgg aaaaattaac tcaggatgtg
    14161 ctcgatatcg cacgcgaaca tcactgggtg acgtatgctg aagtggagat cgataaactg
    14221 cacgcgctgc gctacaccga ttcggtatcc atgaccttaa gctggcagcg ttaaccacca
    14281 tatcgggagg ctgcatgaac atagtgatca ccggagggac gggattaatt ggtcgccatt
    14341 tgattccacg tttgctggac ctgggccatc aaatcacggt agtgacgcgt aacccgcaga
    14401 aagccagttc cgttctcggc ccccgagtga cactatggca agggcttgcc gaacaaagca
    14461 acctcaacgg cgttgatgcg gtaatcaacc tggccggaga accgattgct gataaacgct
    14521 ggactcacga gcgaaaagag cgtctctgcc aaagccgctg gaataccacg caaaaactgg
    14581 tcgatttgat taatgccagc gacacgccac cgtcggtact catttccggc tcggcaacgg
    14641 gctattatgg cgacttaggt gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat
    14701 ttacccataa actctgcgcc cgctgggaag agattgcctg tcgggcgcaa agtgacaaaa
    14761 cgcgagtgtg cctgctgcgt accggcgtag tgctggcacc agacggtggt atcctcggta
    14821 aaatgctgcc gccatttcgt tttggcctgg gcgggccgat tggctccggt cggcagtatc
    14881 tggcctggat tcatattgat gatatggtca acggcattct ctggctgctg gataacgagc
    14941 tgcgcgggcc atttaatatg gtttcgccct acccggtacg caatgaacaa tttgcccatg
    15001 cgctcggtca tgcgctgcat cgcccgacca ttttgcgcgt ccccgcgacc gccattcgac
    15061 tgttaatggg cgaatcttca gtactggtat taggtggaca acgcgcgctg ccaaaaaagc
    15121 tggaagaagc gggttttgcg tttcgctggt acgatttaga agaggcgctg gcggatgtcg
    15181 ttcgctgatg tggtttactg caaacatccg ccagcggaaa cgctgatgaa taaatataga
    15241 taattcagaa ctatttttag cggactgcaa cttttacatt tcagcggaag cggactcgca
    15301 aattccccat gaaatactcc ggcagcgtta accaccggag aggaaattat ttcagcgaac
    15361 ctttaaggaa ctgttgcaga cgagggcttt gcgggttacc gaataactgc tccggcgccc
    15421 cttcttcttc tattttcccc tgatggagga aaatgacgtg agtagaaaca tgacgagcaa
    15481 agcccatttc gtgagtcact accaccatgg ttttcccctc ttctgccagt tgctgcataa
    15541 tacgcaacac ttcgcctacc agttcaggat cgagcgctga agtaggttca tcaaacagta
    15601 aaacttccgg ttccatcgct agcgcccgcg cgatagaaac acgctgttgc tgaccaccgg
    15661 acaggtgcac cgggtatttc ccctgcgcac gttcgtctat cccgactttt gccagatact
    15721 tcaccgcccg ctcgcgcgct tcctgcttgc tcaggcccaa cacctgaatc ggcgcttcca
    15781 tgacgttttc cagcaccgtc atatggctcc agagattgaa gtgctgaaat accatcgtca
    15841 ggcgtgtgcg caataagcgc agctgatttt tatcggcgac tttgagttga ccatctttat
    15901 cgcgcaccag attgatcgtc tggccattga ccacgatcga tccatcgctc ggtttttcga
    15961 ggaagttaat gcagcgcaga aaggtacttt tccccgatcc cgacgatccg atgatgctta
    16021 tgacatctcc ggcattcgct tgcaatgaga cccctttcag cacttcgtgt tcgccgtagc
    16081 gtttgtgcaa atcgataacg tttaatttat tctcggacat cgtattctca gtgcgttgaa
    16141 gaaggtttca catgctgcaa ccagcgtttt tcagctctac gaaagaggct gatcagaaca
    16201 taagagatga ttaaatagag caccgcggca atgccgaaag cggtaaaagg ttgatatgtg
    16261 gcggcgttaa tatcgcgggc aattttcagc agatccggca ccgtggcagt aaatgccagc
    16321 gcagtagagt gcagcatcag gatcacttcg ttgctgtatg ccggtaacgc aatacgcagc
    16381 gcagaaggca aaataatgca gcgatacatt ttaaaggtcg agaagccata ggctctggcg
    16441 gcttcaattt ccccatgcgg cactgaacgg attgccccgg caaaaatctc ggtagtgtaa
    16501 gcgcaggtgt taagcgtcag cgccagcacg gtacagttca ggccactgcg aaaaaaggcg
    16561 ttaaggaact cggttccctt aacaatctcc agcgtgtaca tgccggaata gaacaccagc
    16621 aactgaacat acagcggcgt accgcgaaaa atataggtaa ataaccagat tggaaactgg
    16681 atgtatttat tacttgagac gcgaccaatc gccagaaaca gcgccagaac tccgcctatc
    16741 actaccgaca aaatcagcag ccacagggtg atcgccacgc cagtaaagcg ataaccgtca
    16801 gtccacagca gtggtttcca gtattcatgt aagatttcga tcacaggtca gccctcttca
    16861 cacccacgga gtagcggcgc tcaaggaaca gcagcacacc attggaaacg gtggtgaaaa
    16921 caagataaat cacgccgcag acgatagcga aatagaacgg ttcccaggta cttttgcctg
    16981 ccagctgcgt ggctttgacc acatcttcca ggccgagtaa cgaaaccagt gcggtagatt
    17041 tgaggatcac ctgccagttg ttgccaatgc ctggcagggc gtaacgcatc atcgccggaa
    17101 acatgatccg ccgaaacact tgcccacgag taaaaccgaa cgccgtcgcc gcctctatat
    17161 gtcctttcgg cactgccata aacgcgccac gaaacgtttc ggtaaaataa gcaccgtaga
    17221 taaaaccgag agtgataata cctgcgacca tcggatcaat atcaatctgc ccgacgccca
    17281 tcgcctccgt caccgtgttt agcgcaatct gcaaaccgta gaaaatcagc agcatcaata
    17341 ctaaatccgg cacgcctcga atcagcgtgg tgtacccttc gaaaataagg cccgaaagcc
    17401 gattttgtga gagcttaccg ccagcgccaa ttaaaccgat gattacagcg agcactacag
    17461 agctgatagc cagctccagc gtgacgagcg caccctgtaa aataacactt gaaaacccat
    17521 acaacatgct gcctgtcctg tcgtgtgtgg tgaattaccg cctcttgtct ccctccggtt
    17581 acccgaaggg agcgggcttt ctttcaccga tggcgattaa ccaccataaa catcaaaatc
    17641 gaagtacttt ttcgctaatt tctcgtaagt accgtcagcg cgcatttcgg caaaggcttt
    17701 gttcagtgcc tcgcgcagtt cgttatcttc tttacgcagg cccatgccgg tccctacgcc
    17761 aaacagtttt tcatctttaa cagacgggcc accgaatttg taatctttac cgacgggttg
    17821 tttgaggaaa ccttcgctgg ctgcgacctc atcctggaac gcggcatcaa tacgtccggc
    17881 agtcaggtca gaataaatgt tgtcctgccc ctgataagaa acgatttcaa tgccttttgg
    17941 tgcccagtgt tcgttaccga acgtctcctg ggtggtgccc tgcaatacgc ctacccgttt
    18001 gcctttcagc gattcgactg tcggctgaat gtcggaattt ttcgccacca ccagacgaga
    18061 atcggctgcg tacagtttgt cggtgaaggc tatttcttgc tgacgttttt ccgtaatgga
    18121 aagcgatgac atgatggcgt caatcttctt cgcttttaag gacgggatta acgcatccag
    18181 tgggttttcg acaaacgtac attgcgtatt gatgcgtttg cacaactcct tggccagatc
    18241 gatatcgaag ccaaccagtt caccttgtga attttttgat tcaaatggcg cataggtcgg
    18301 atcggtaccg atgcggatgt tttgcggaat cgcagcaaat gctgcagttg cgctggagaa
    18361 ggccagaacc agagagagcg atagcaccag ttttttcata atgtcctcaa ctgacagtct
    18421 ttttatcggg aatatttaca ggtttgttac acttatcgtg ccataaaatt gaccatcaag
    18481 gcaaaatatc ccgccccgct agacgtatta tttttcatta aaaatgctta agtatgcatt
    18541 tcaatgcacc acgatagtgc aacaaacctg gcgtgcacca tgatgatcat tccatcaggt
    18601 acagcttccc tgcgacgtat cagtcaccgt agacattaaa gtcaaaatac tttttcgcca
    18661 tcttgtcgta ggtgccgtcc tgacgcagct cgccaagcgc cttattaaac gcagccgtca
    18721 gttccgcatc atctttacgt agccctacac cggtgccgtc accgaagtat tttttgtctt
    18781 tcactgatgg gcctgcaaag gcgaaatctt taccggcggg ttgcttaagg aaaccttcgc
    18841 tggcagcaac ttcatcttgt aacgcagcat ccagacgtcc tgcagccaga tcggaataga
    18901 ctaaatcctg gttggcatag gccaccacat ccacgccttt gctacgccag gtctcgttag
    18961 cgtaagcttc ctgggttgat ccctgcagca caccaacatg tttacctttc agtgaatcca
    19021 gcgttggctg aatcggtgaa cctttggcgg caatcaaacg agaatctgcg gcgtacagct
    19081 tgtcggagaa agcaatctcc tgctgacgtt tatcggtaat ggagagcgac gaaataatgg
    19141 cgtcgatttt tttcgctttc agtgagggga tcagcgcgtc aaagtcgctg gcaacccagg
    19201 tacatttcac ctgcatccgt ttgcacatct cgttaccgag atcgatatca aagccaacaa
    19261 agtcaccttt ggcatctttc gatgagaacg gtgcgtaggt ggtatcggtt ccgatacgta
    19321 ccgtctccgg tagcgccgca tagctggaag ccgctgcgga gagaccgact aacaaagaca
    19381 gagcgagaat cgacttcttc atacataacc ctcaagtgaa aatggctttt ttatgttgtg
    19441 tattgtgttg tgtgtttgca ggctctttca tgcaggtctt atgccatctt gacgacagca
    19501 gtaacattca acgttaaata tgttaataag aagttgcatt attgtcctaa agttgaagat
    19561 agcaggtata gcggttgaat cgcagcgttt cgattgaatg gcagaaacaa aatgtcgagg
    19621 atttgatcgc ggttgcaaaa ttgccctgaa acagggcaac agcggagtta tgcgccctgc
    19681 cagcgggcaa agagatcttc aggaagggtt accgcaaact ggtcaagaac acgattaacc
    19741 gtctgattta tcacatcatc aagggattgt gggcgatgat aaaacgccgg aacgggaggc
    19801 ataatcaccg caccgatttc tgccgcctga gtcattaaac gcagatggcc taagtgcagt
    19861 ggcgtttcac gcacgcagag caccaacggg cgacgctctt tcagcaccac atctgccgca
    19921 cgggtcagta agccatcagt atagctatgg acaatgccgg acagggtttt gattgaacag
    19981 ggtaaaatca ccatccccaa cgtctggaaa gaaccggaag aaatgctggc agcaatatcg
    20041 cgcgcatcgt gtgtcacatc agctaacgcc tgcacttcgc gcagggaaaa atccgtttcg
    20101 agggataagg tctggcgcgc cgcctggctc attaccagat gcgtttcgat atctgtgaca
    20161 tcgcgcagaa cctgtaataa acgcacgcca taaatcgcgc cgctggcacc gctgatgcct
    20221 acaatgagtc gtttcataaa tagttgccct gtcagacttg caggcagact ttgcaggatt
    20281 tcggcgggag ttgcaagtca gggtgccaga ccggcaccct gagcgaaggc atcatccttc
    20341 gttatgcatt tcgagatttt ccacttcgtt ctgacgttgc actgctttgg cgtcgtcatt
    20401 acgtaaagta tcgaggaaat cgaggtagcc ctgatcaacg tctttggtga cgtagacgcc
    20461 gttgaacacc gagcattcaa actgctggat atccgggttt tcagcgcgaa cggcttcgat
    20521 cagatcgttc agatcctgga aaatcaaccc gtcagcaccg atgatctggc gaatttcatc
    20581 tacttcgcga ccgtgagcga tcagttccgt ggcgctcggc atatcgatac cgtaaacgtt
    20641 cgggaagcga atttccggtg ccgcagaagc gaggtacact ttcttcgctc cggcttcgcg
    20701 tgccatctcg ataatctgct cagaagtggt gccacggacg atagagtcgt cgaccagcag
    20761 gacgttttta tcgcggaact cggcgcggtt ggcgttcagt ttacggcgca cggacttacg
    20821 acgcagctgc tgacccggca tgatgaaggt gcggccaaca tagcggtttt taacgaagcc
    20881 ctggcggtac ggtttgccca gaatacgcgc aatttccagc gcgatatcgc aggaagtttc
    20941 cgggatcggg atcaccacgt cgatatccag atcttcccat tcgcgggcaa ttttctcgcc
    21001 cagcttcgtg cccatattca cacgcgcgct gtaaacggaa attttgtcga tgaacgagtc
    21061 cgggcgagca aagtatacat actcaaacag gcacggattg ctgaccggat tgtcagcaca
    21121 ctgacgggta aacaactgcc cttcttcagt gatgtaaatc gcttcgccag gggcgacatc
    21181 acgcaggaac tcaaagccca gcgtatcgag cgcaacgctt tcggaagcga ccatatattc
    21241 tgtgcgatta tcgtcaatat cacgtttgcc cagtacaagc ggacgaatcc cgttaggatc
    21301 gcggaaagca accataccgt ggccgataat catcaccaca caggcatacg cgccgcggat
    21361 taagcggttt gtagcagcga tggcggcgaa aatgttgtcg gcttccagcg ggtagtggcg
    21421 gaagttgtcc agctcgctgg cgaagatatt aagcagaatt tccgagtcgg aagtggtgtt
    21481 gatgtggcgg cgtttttctt caaacagttt tttacgcaac tcgtgagcgt tggtcagatt
    21541 gccgttgtgg gcaagcgtaa tgccatacgg agagttaacg taaaacggct gcgcttcaga
    21601 ggcgctggag ctgccagccg tagggtaacg cacatgacca atgcccatat tgccctgcaa
    21661 acgctgcata tggcgagctt caaatacatc gctcaccagc ccgttcgctt tccgcaaacg
    21721 gaagcagtta ttggcatcta tggtgatgat gccggcggca tcctgaccgc gatgctgaag
    21781 caccgttaag gcatcataaa tcgactggtt aaccggcata acaccggcga taccgacaat
    21841 accgcacata cgtctttttc ctcgttaagc cacatctcag agcacttacg ctctgggcaa
    21901 gaaacttgac gagctttgca gataatcaaa aaaccatctg atgataaaac tgaactgcgg
    21961 gatcagctgt gatttgctcc agtcttcgct tttcgacacc ccggtaaagg agtcgagaaa
    22021 gaagagaatg gcagcaacaa tcaacacacc gcgcaaggca ccgaaacaga cgcccaacac
    22081 ccgatcggtg cctgacaacc ccgttttctc caccaactgg cctatcacga agttcacgat
    22141 agcaccaacg atcagggtag cgataaacag taccgcgatg gcaatcccat ttcgaaccag
    22201 ttcgtcttca aagcccgtaa accagactga caggtaagtg tagtaatgac tggcaacaaa
    22261 gaaagcacaa ccccatgtca ccagcgataa cgcttcacga acaaagccgc ggatcaggct
    22321 aaccagagag gaaaaagcaa tcaccgcgat tatggcgtaa tcaatccaga ccatatgtgt
    22381 cccacgattt tacgccctgt catcctgttc ggggcgcatt ctaacagaaa aagaaaacgt
    22441 ttgcgtaggg atttccttcc cgcgcatcaa taaaaatggc gctgaaaaaa tattcaacgc
    22501 catcgacttt ttatgccttt gcggcatcgg gcaatgcgtg tcggatgcgg cgtgaacgcc
    22561 ttatccgacc tacggctcta cccctgcaca ggcctgataa gacgcactgg cgtcacatca
    22621 ggcaacggct gtcggatgca gcgtgaacgc cttatccgac ctacggttct acccctgcac
    22681 aggcctgata agacgcgctg gcgtcgcatc aggcaacggc tgtcggatgc ggcgtgaacg
    22741 ccttatccga cctacggttc tacccctgca caggcctgat aagatgcgcc agcgtcgcat
    22801 caggcaaaac cgtattaatt cggtgtatag cccattacca caccacttaa gccagaaagc
    22861 tgcttgagct cacccagcga tcctttcagc ttctctttgg aagcatctgg cccaaccaga
    22921 atacgggtaa ttttaccctg cactggcgtg gatggcgacg tataaacccg ataaccggca
    22981 ccgcgcagct taccgacaat ctcattcact ttatcggcat ttttcagcgc gcccagttgc
    23041 acaacatagg ctttacccgt tggcgctgct ttttcttcca cgaccggctt cggttctggc
    23101 ttcggcgcag gtggtgcttc aaccttcggt ttaggcgact ccaccggttt cggcttcggt
    23161 gggacgaccg gtgcaggttc cggttcaaac tcggtgttat tggctgcaat agtggccgga
    23221 tcgagcgacg gtgctgcggc atcgcctgcc cgcacctctt ccgctgcgcc ttccggcggc
    23281 tgcgtcggca acgcctgggt ggcggctggc atcatatcag gctcatcgcg atcgcccgct
    23341 ttcggcacca gcgggatagc cgcgaactca tcctgataat gttttttctg cccgtccagc
    23401 agccctggaa gtacaatcac ccccagcgcc accagcacga tcgtgcccac taaccgattc
    23461 tgaaacttac ttgccaccgc ttctcctcgc gtcaatcact tccatgacat gtgcgaccgt
    23521 gtggaacgaa ccacacacca gcacggtgtc ttccgcttta gcgtccgcca ttgcggcatc
    23581 ccatgcctgc gcaacgctat caaatgattt gccgttaccc aaatgctcaa gcagttgttc
    23641 tgccgtggca ccgcgcggcc cttccagtgg cgcacaatac cagtcatcaa ccacgctttt
    23701 caaccaggcc agagttccgg caatatcttt atcatgtagc ataccgataa ccgccagcac
    23761 gcgcccgttt ttcggtagcg ctttcatacg cccggtgaga tattccgccg catgtggatt
    23821 atgcgcgaca tcaaaaataa cgcgtggcga ctcgctcaca atctggaaac gtcccggcaa
    23881 aattgcgctg gcaatcccgt cgcgaatggc attttcactg acctccagcc cgctggcacg
    23941 cagtgccgcc agcgccgttg cggcattcgg ttgcgggaca agcggcaacg gcagattttc
    24001 cagcgtgccg tgagcatcgc taaacgtcca gtcatgatcg gtgacggaat agttccactc
    24061 aacgccccga cgttgtagca gtgcaccttt ttcctgcccc acatcagcaa tggtagaagg
    24121 catttccggc tcaccgacaa ttgccggttt ttcgctgcga aagatacctg ctttctcgcg
    24181 accaatactt tcgcgatctg gccccagcca gtcggtatga tccagcgcaa tactggttac
    24241 taccgcgaca tcggcatcga caatattggt tgcgtccaga cgaccgccca gccctacttc
    24301 cagaatcact acgtcaagtt gtgcctgctt gaacagccac aatgccgaca gcgtaccgta
    24361 ctcgaaatag gtcagggaaa tatcaccgcg tgccgattca atctccgcaa aagaagcggt
    24421 gtgggccgat tccggcaatt cctgcccctg cacacgtacg cgctcggtat aacgcaccag
    24481 atgcggcgaa ctgtagacgc ccactttgta ccctgccgcc atcagaatcg actccagcgt
    24541 acggcaggtg gtgcctttgc cattcgtacc cgcaacggta aacacaaatg gcgctggttt
    24601 cagaacgcca agacgcgccg cgacctggct cacgcgctca aggccgagat cgatagtttt
    24661 actgtgcagg ttttccagat aagaaagcca cgaagccaga ggcgacgcgg cttgaggagt
    24721 gcgtttgata atcatggtat ccgctgattc gttacggtga gaatagcaaa agggcagagc
    24781 cagtggccct gcccttatca gttatcaggc ctcaggttcc tgatccggta ccggtggcac
    24841 cactacgcct tcacgcggcg cttcaggatt cggcgctggc agattcatca acttcgccag
    24901 aatgctcgcc agtttcagac gcatttccgg acgacggacg atcatgtcga tcgcgccttt
    24961 ctcgatcagg aattcactgc gctggaatcc tggcggcagt ttttcgcgaa cagtctgttc
    25021 gataacacgc ggaccggcaa agccaattaa cgctttcggt tcagcgatgt tgagatcgcc
    25081 cagcatggcg aaacttgcag aaacaccgcc catggtcggg tcggtcagca cggagatgta
    25141 cggcaagccg cgctcctgca tttttgccag tgccgcagag gttttcgcca tctgcatcag
    25201 cgacatcagt gcttcctgca tacgtgcgcc accagaggcg gagaagcaga tcagtgggca
    25261 gttatcttcc agcgcctgct caacggcacg cacgaaacgc gcacctacca cagaccccat
    25321 ggaaccgccc ataaaggcga actcgaatgc cgcagcgaca accggcattc catacagagt
    25381 gcctttcatc accaccagcg catctttttc gccggtttct ttctgcgcag atgccagacg
    25441 gtctttatac ttcttggagt cacgaaactt cagcacatct ttcggctcaa gctcgctacc
    25501 cagctccaca aggcttcctt catctaacag gctatgcagg cgattacgcg ctgtcatacg
    25561 catgtgatgg tcacacttcg gacagacctc aagattacgt tccagctcag cgcggtataa
    25621 aacctgaccg cagctatcac acttagtcca caccccttca ggaatgctcg ccttgcgggt
    25681 gggagtaatg ttgcttttaa ttcgttcaat ccagctcatt agggaccttt ctgtctgaac
    25741 ctggttcgat gccagtttta tctttgggga cgcataatgc catttttgcc cccaacagac
    25801 catgaatgtt gcacattaaa acataacagc ccgaaacttt ggataaaaaa gtggtcgaac
    25861 cgcagagtta ctttttattt tgcggcgcgt gccgctgcgc gtttgtggcg aataaattcg
    25921 aaaacacctg gcaatattga aacaaaaata atgccaacaa tcatcagttt aaggttgctc
    25981 tgaatgaagg ggagcgtgcc gaagaaatag cctgcgtagg taaaaagcag tacccacaac
    26041 agtgcgccaa tcacgttata agcggcgaaa tgacggtacg acatgtggcc cattcccgca
    26101 acaaacgggg cgaacgttct gacgatcggc acaaaacggg cgagaataat cgttttgccg
    26161 ccatgtttct cataaaactg atgggttttg tcgagataac tgcgacggaa aattttcgaa
    26221 ttgggattac tgaataactt ttcaccgaac aaccgcccga tagtgtaatt gaccgcgtca
    26281 cccacaatcg cggcgatcag catcagcacc accatcatat ggacgttgag atcgttggtc
    26341 tccagtgatg ccaacgctcc ggcaacaaac agcaatgaat cacccggtaa aaacggcgtt
    26401 accaccagac cggtttcaca gaacaaaatt aagaacaaaa tggcataaac ccagacgccg
    26461 tactccgcga ccagctctgc cagatgcacg tcaatgtgca ggataaaatc gatgaggaaa
    26521 taaatcaggt ccatatttgc ctatgccttg tactcgtcat ctatcaggct gtaactacgt
    26581 tggctacagc ctgaaatgtt ccgagcatta ttctcgttag tccgccagaa atagcgggcc
    26641 cattggcggt tttggaagat catatcggtc agggtaatcc accgcgacca gatatagccc
    26701 ttccgctttt gccgttgctg ccgccagcgt tctgtccttt gccgccagca actctgctat
    26761 ccagctctcc ggctggttgt gggcaccgac ttccatcagg ctgccgacaa tattcctgac
    26821 catatgatgt acaaaggcat tcgctttgat atctaccacc acataaggac cgtgacgcgt
    26881 gacattaata tgcataacgt tgcgccacgg ggtccgggac tggcactgca ccgcacggaa
    26941 cgaggtgaaa tcattctcgc ccagcaaaca ttgcgcagcc cgatgcatcc gttcagcgtc
    27001 cagcggttcg taaaaatggg ttaccccttt actcagtacc gccgggcgca gccgatgatt
    27061 gtagatgatg tagcgataac ggcgagccgt ggcgctaaat cgggcatgaa aatcatcagg
    27121 tacagcttta acccaacgca cggcgatgtc accaggtaaa ttcgcattta cgcccaacgt
    27181 ccacgcagcg tctttgcgct gcgcggtcgt ttcgaaatgc acaacctgcc cggtaccgtg
    27241 tacaccggcg tcagtacgcc cggcgcagaa gacggtgatg ggttcgttcg ccacctggga
    27301 gagcgccttt tccagcttct cctgcacact gcggacttca ttctgccgtt gccagccgta
    27361 atacctactg ccgtcgtact caatgcccag cgcaatttta taaactggcg gttgttgctg
    27421 gtcggacatt agtacagata ctcctgcacc agtttctcgg caatttttac tgccatcagc
    27481 gcgccgccaa agcgaacgtt atcggccacc gaccagaact gaacttgctc cggcataccg
    27541 tagtcattac gtacgcagcc aacagaaaga tgcggcgtac cagaagcatc ccctacctga
    27601 gtcgggaatt cgttctcttc agagagcaca atatcttcgc cctgagcaaa cgcatcacgc
    27661 gcttcttctg ccgccagcgg acgcagtgct tcaaagttga ccatctgggc atgaccgtag
    27721 aataccggtg cctggacgac gcttgccgaa atcatcagcc cttcgtcctg caggattttg
    27781 cgtacttcgt caacgatacg acgttcttca cgcacgctac cttcgctatc cggcagtaac
    27841 ggcagcatgt tgaacgccag ctgacgcccg aagaaatctt cttcgtcaat cggaatgcca
    27901 ttgagtaatt tagcactctg ccccgctaac gcatcgaccg cttttttgcc ctgggcggag
    27961 gctgaaatca ggctggtaac gctgatacgt gataaaccgc cctgatcaat tagcggtttc
    28021 agtgccgcca gcagctggct ggtcagactg tctggtacgg cgatgacatt ccggttgcgg
    28081 taatcggtca gtacaaacgg gtttacttcc ggcaccacca gcggtacgtc gggttcgaga
    28141 gcaaacaatc cgctgctgtc gatcaccaga caacctgagt tggtcgcttc ttcaacccag
    28201 gcagcggtag cctctttgcc tgcgacaaaa aatgccagct gcgcctgcgt ccagtcgaat
    28261 tcagcagcat cctgcacggt gattgtctta ccaccaaagc gcagttgttc gcctgcgctt
    28321 tcgttacgtg ccagtgcata aatttcccca accgggaact gacgttcagc cagcgtttca
    28381 agcagggctt cgcccacagc gccagttgcg cccaggacgg caatgttcca gccttcagac
    28441 atggtggttt actccagaaa tagcaaagct ccctgccaat attacagcag agagcatgaa
    28501 gaagagatta acgtgccgga tgatgaacgg cgttaaaacc cagtttacac agcaatgatg
    28561 ccgcgctggc gtcatcacaa atcacataca gagatgacca ttcacggcgc tcaagatagt
    28621 ttttgcgcag cttatcgaac tcacccggta ttccggcgac tttacgcagc ggtgcgtcat
    28681 cgcggcgcac atcatacacc aaatgcacca gcctttttag cgtcggttga tcgagcggac
    28741 catgcagcgt aatgcggcca aactctggcg cgggcagcaa cgtatccagc gcaacgtgtt
    28801 gttcatgccc aataagcttg ctataggctt caaacacttg cgtagtaccg cgtgctttac
    28861 cttccagggt atagcctgcg atatgcggtg tgccgatatc cacttttttc agcaattcca
    28921 cgttgagttc cggttcgcct tcccagacat ccagcactac gcttaacttc tggccttcgt
    28981 tcagacaggt caatagggca gtattatcga cgactgcgcc acggcaggcg ttaatcagaa
    29041 tcgctcctgg tttcaggcta cggatcagtt tttcatccgc cagatgcagc gttttgtacg
    29101 gcccgtcttt gaagagtggc gtatggaaag tcagaatatc cgcatgctga actaattcat
    29161 ccagcgagcg gaaatccccc tcatccccgc ggtcggcgcg aggcggatcg caaagcaagg
    29221 ttgtaatccc taacgcttcc agtcgcgcct gtaaacggcg accaacgtta ccaacgccca
    29281 cgatccccac tgtacggtcg tgcagtgaaa atccatcgcg ttcggcaagc atcagcagtg
    29341 aggagaaaac atattccacc accgcaatcg cattacagcc aggtgcagcg gaaaaaccaa
    29401 ttcccgcctg ctttaaccat gcttcatcga catggtcggt ccccgcggtg gcagtgccaa
    29461 caaatttaat gggttttcct gccagcaaag attcattcac tttcgtgacc gaacgcacca
    29521 taagcgcatc cgcgtctgcc agttgagcga caggaattgg acgcccggga accgcggtca
    29581 cctcacccaa acggctaaat aagtcgcggg cataaggcat attttcatca acaaggattt
    29641 tcacgtttgt gttacctgta tgagacgaga gttaaccaga caagtgtgcc ataatctcgc
    29701 ggccaggcat acttgcgaag atttcaggta taaggatacg taatgataca acctatttcc
    29761 ggccctcctc ctgggcaacc accaggccag ggagataacc tgccgtctgg cgcgggcaat
    29821 cagcctttat ccagtcagca acgtacttcg ctggaaagct taatgacgaa agtgacctca
    29881 ctgacgcaac aacaaagagc agaactgtgg gcgggtatca ggcacgatat tggtctgtcg
    29941 ggagattcac cgctgctttc gcgtcacttc cctgccgctg agcacaatct ggcgcaacgt
    30001 ctgctggccg cgcaaaaaag ccattctgcc cgccagcttt tagcgcaatt aggggagtat
    30061 ttacgtctgg ggaataatcg tcaggcggtc acggattata tccgtcataa ctttggtcag
    30121 acgccgctga atcagctctc accggagcaa ttaaagacca ttctcaccct gttgcaggaa
    30181 gggaagatgg ttattccgca acctcagcag cgcgaggcga ccgaccgtcc gttattaccg
    30241 gcggagcaca atgcgctcaa acagctggtg accaaacttg cggcggcaac gggagaaccc
    30301 agcaagcaga tctggcaatc gatgctggaa ctttccgggg tgaaagatgg cgagttaatt
    30361 ccagcgaaac tgtttaacca tctggtgacc tggctacagg cgcgtcagac gctaagccag
    30421 caaaatacgc cgacgctgga atcactccag atggcgctaa aacaaccttt agatgccagt
    30481 gaactggcgg cgttatcggc atatatccag caaaaatatg gcctttctgc gcaatcatcg
    30541 ctttcttctg cccaggccga ggatattctt aatcagcttt atcaacggcg ggttaaaggg
    30601 attgagccgc gtgatatgca accgctgctt aatccttttc caccgatgat ggacacgttg
    30661 caaaatatgg caacgcgtcc cgcgctgtgg atactgttag tcgcgattat cctgatgctg
    30721 gtctggctgg ttcgttagcc ccgacgaaat gacagtatcg tgacaataat tcccagcacc
    30781 gcagagatcg ccccggcaag aaataccgaa gagtaaccaa acgtggtcgc cagcattccc
    30841 gccagcggcc cggagacgcc gagggcgata tcctgaaacg cggcgtaacc gcccagtgcg
    30901 gtgccgcgaa cttgtgaggg gacgcgttta accacctcca cgcccagcgc aggaaagata
    30961 agcgaacatc cggctccggt taacgccgcg cccgctaatg cgacccacgc accaggggct
    31021 tgccagagca gcaacaagcc caccgtttct acaagcagag agacgatcgc cactttcacg
    31081 ccgccaaaac ggtccggcat ccagccaaac atgacgcgca tcacgacaaa tgcgccgcca
    31141 aacgcggtaa gagtaaagcc cgccatcgcc catcctttac tggcaaagta gagcgaaacg
    31201 aaagtcccga taaccgcaaa accaacgcct tgtagtgcca gacctaaccc tggtttccag
    31261 ataagcccga caacgctcca cagcgatgga cgttctcccg ccagggccgg tactttgcgc
    31321 actgtgccgt tacaggccca cgccagtaag ggtaatgcca ttgtggtgag cgccagtgcg
    31381 gcaaaaccgt aatggctatg aatcaacagg ccaagcggcg caccaacagc gagggcaccg
    31441 taaatcgcca ttccgttcca tgacatcact ttgccggaat gttttggccc aacgatgcct
    31501 aatccccagg tcagagcgcc cgtcagtaac tggctttcac caaagccaag aatcaaacgc
    31561 ccgacgacca acagggcaaa tttgaacggt gcggagacgg gcaaaatcgc cgccaacagc
    31621 agcgcgccgc cagccagacc acaagctaac atcccctgaa gcgccgaacg ttttgcgcca
    31681 tattgatcgg ccagtcgccc ggcgtaacca cgcgtcagca ccgtagccag aaactgaatg
    31741 ccgacggcaa taccgaccat ggtgttgcca tagcccagtt catgatgaac aaacagcggg
    31801 ataaccggca acggcagccc tacggtcatg taggtgagaa aaaccgcaaa agcgatgcgg
    31861 aagagcgaaa aattggcaga agatcgtgtt tcggtttggc ttacagcagt catgcattac
    31921 tccagaatgc agcgcaaggc gaggaatttc cccgtctcat ctctctggtt tcagggttac
    31981 ggtgcgttgg caggatttaa cgcgtacgtc ttttcagaag gaaatcgaca aagcgggaag
    32041 tttgcctgga acaggcggcg attgtcaata atgtgaaaaa gggaaccatc aggttccctt
    32101 ttgcgttagt gtcggatgcc gcattggcgc gtaatgtcgg atgcgacgct ggcgcgtctt
    32161 atccgaccta ctccgaatta atctttcagc ttgcgcatta ccagcgtggc gttggtgccg
    32221 ccgaagccga agctgttaga cataactgtg gtcagttcgc gatcggtcgt ttcggtcacg
    32281 atgttcagac ccgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaaa
    32341 ccgtgttcca gcatcagcag agagtagata gcttcctgta cgccagcagc gcccagagag
    32401 tgaccggtca ttgctttggt tgcagaaatc gccgggctct tatcgccgaa cacttcacgg
    32461 atagctgcca gttctttcac gtcgccaacc ggagtcgaag taccatgaga gttcaggtag
    32521 tcgattgggg tatcaacacc gtgcatcgcc atcttcatgc agcgcactgc accttcgcca
    32581 gacggagcaa ccatgtctgc gccatcagag gttgcgccgt agccaacgat ttcagcatag
    32641 atgtgagcac cacgcgccag cgcgtgttcc agctcttcaa ccactaccat accgccaccg
    32701 ccagcgataa cgaaaccgtc acggtgagcg tcgtaagtac gggaggcttt ttccggggtg
    32761 tcgttgtatt tagtagacag cgcacccatc gcgtcgaatt cgcaagccat ttcccagcac
    32821 agctcttcgc cgccgccagc aaacacgatg tcctgtttgc ccagttggat ctgctctact
    32881 gcgttaccga tacagtgtgc ggaagtcgca cacgcggagc tgatggagta gttaacgccg
    32941 tgaattttaa acggcgtggc gaggcaggca gaaacgccgg atgccatcgc tttggtgacc
    33001 acatacgggc caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc gccgaaaacc
    33061 tggaaacgcg gggagccgcc gccggaacct gcaatcaggc caacgcgcgg gttattctgg
    33121 taagcttccg gggagaggcc agcatcagca attgcctgct ccatagaaag gaatgcataa
    33181 atggatgcgt cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt
    33241 tttacgttgc cccagacgtg gctacgcatg ccggaatcct tcagctcctg agagaaagtg
    33301 atccctgaac gtccttcacg cagagatgcc aggacttcct gctggttatt accgatgctg
    33361 gaaacaatgc ccaggccagt aatcactgca cgtttcattc aatacctctg taagtcgcac
    33421 atagagtaag tttcgaatgc acaatagcgt acacttgtac gccgaacaag tccgatcagc
    33481 caattaatag agaaatttgc gcagcctgac acacatcgct aagatcgagc caccgcctgt
    33541 aagacgagta acttacgtga aacactactc catacaacct gccaacctcg aatttaatgc
    33601 tgagggtaca cctgtttccc gagattttga cgatgtctat ttttccaacg ataacggact
    33661 ggaagagacg cgttatgttt ttctcggggg taaccattta gaggcacgtt ttcctgaaca
    33721 tccacatcct ctgtttgtgg tagcagagag cggcttcggc accggattaa acttcctgac
    33781 gctatggcag gcatttgatc agtttcgcga agcatatccg caagcgcaat tacaacgctt
    33841 acatttcatt agttttgaga aatttcccct cgcccgtgcg gatttagcgc tggcgcatca
    33901 acactggccg gagctggccc cgtgggcgga acagcttcag gcgcaatggc ctttgccctt
    33961 gcccggttgc catcgtttat tgctcgatga aggccgcata acgctggatt tatggtttgg
    34021 cgatataaac gaactgacca gccaactgga cgattcgcta aatcaaaaag tagatgcctg
    34081 gtttctggac ggctttgcgc cagcgaaaaa cccggatatg tggacgcaaa atctgtttaa
    34141 cgccatggcc cggctggcgc gtccgggcgg cacgctggcg acctttacgt ctgccggttt
    34201 tgtccgccgc ggtttgcagg aagccggatt cactatgcaa aaacgtaagg gctttgggcg
    34261 caaacgggaa atgctttgtg gggtgatgga actgacatta cccctcccct gctccacgcc
    34321 gtggtttaac cgtacgggca gcagcaaacg ggaagcggcg attatcggcg gtggtattgc
    34381 cagcgcgttg ttgtcgctgg cgctattgcg tcgcggctgg caggtaacac tttattgcgc
    34441 ggatgaggcc cccgcactgg gtgcttccgg caatcgccag ggggcgctgt atccgctttt
    34501 aagtaaacac gatgaagcgc taaaccgttt tttctctaat gcgtttactt ttgctcgtcg
    34561 gttttacgat ttattgcccg ttaaatttga tcatgactgg tgcggcgtca cgcagttagg
    34621 ctgggatgag aaaagccagc ataaaatcgc acagatgttg tcgatggatt tacccacaga
    34681 actggctgta gccgttgagg caaatgcggt tgaacaaatt acgggcgttg cgacaaattg
    34741 cagcggtatt acttatccgc aaggtggctg gctgtgtcca gcagaactga cccgtaatgt
    34801 gctggaactg gcgcaacagc agggtttgca gattcattat caatatcagt tacaggattt
    34861 atcccgtaag gatgacggtt ggttgttgaa ttttgcagga gatcagcaag caacacacag
    34921 tttagtggta ctggcgaacg ggcatcaaat cagccgtttc agccaaacgt catctctgcc
    34981 agtatattcg gtggccggtc aagtcagcca tattccgaca acgccggagc tggcgaaact
    35041 gaagcaagtg ctgtgctatg acggttatct cacgccgcaa aatccggcga atcagcacca
    35101 ttgtattggt gccagttatc atcgcggcag cgaagagacg gcgtacagtg atgaagatca
    35161 gcagcagaat cgccagcggt tgattgattg tttcccgcag gtacagtggg caaaagaggt
    35221 tgatgtcagt ggtaaagagg cgcgctgcgg tgtgcgttgt gctactcgcg atcatctgcc
    35281 aatggtaggc aacgtgcctg attatgacgc tacactcgtg gaatatgcgt cgctggcgga
    35341 gaagaaagat gaagcggtaa gcgcgccggt ttatgacgat ctctttatgt ttgctgcttt
    35401 aggttcgcgc ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg cacagatgag
    35461 tgaagaaccg attccgatgg atgccagcac gctggcggcg ttaaacccga atcggttatg
    35521 ggtgcggaag ttgttgaagg gtaaagcggt taaggcgggg taatctgctc tggcattgtt
    35581 tgtcggatgc ggcgtaaacg ccttatccga cctacgcgtt acctgtaggc ctgataagac
    35641 gcgataagcg tcgcatcagg catggtgctc cagacgtcac aacttactgc tgtgaggcct
    35701 gctgaaacag gtgttcccac atatcggtca ccagcgcctg gtcacgcggc gacaattcac
    35761 cggcaccaat ggctttttcc agactctggc taacggttgt atgtaccgcc tgagcggagt
    35821 ggtcgtcacc actttccagt tctgcgatgg ctaatgtcag gtggccacgc aaatacccac
    35881 tggcaaacaa ctcatcatca ctggcatggt caaccatacc gtcgattaat gccagaatgc
    35941 gtgattcaaa ctccgcgatc atcttctttc ctcattgtaa aacgtggcgc tggcttcagt
    36001 tgagcgcttc cggccacggg aactgttccg ccgtaagttc cggcgtgtga taataatttt
    36061 gtagtgcttt aatgaagcgt gccggacgtt cgggaatgcc gtttgccaga taatccatca
    36121 cctgcgcatg tacccgccgt tgaaacacca cgcggtccgg ttcgaaatcc ccttccagat
    36181 tgtcgcagct aacattaaac ggatatcccg ctgccacgca gaacaaccag tccagcgcct
    36241 gcggcttcac ttcaacatct tcaaactggc tttgtgtctg ggcatcgcgt ccgtccgggc
    36301 aataccagta gccgaaatca accagctcac ggcgcgcttt cccggcaata caccagtgcg
    36361 aaatctcgtg gatggcgctg gcataaaagc catgagcgaa gacgattctg ttatacggta
    36421 cttccgcatc agcaggaaga tagatcggtt cgtcgtcgcc tttaatcaga cgggtattaa
    36481 attcatcggc aaagcagcta ttaaaaatct caatcaactg ctcatagtgg tgtgtactgt
    36541 tcattagttc atccccaacc agtggaggat ctcctgtcca tggctatcgt aaagtaattt
    36601 ggcactcatc accgccgaga cgataacgat catcgggcgg atcagctttt gtcctttgct
    36661 caacaccagt cttgaaccca tgcgcgcgcc gaggaactgc cccaccagca tcacaaagcc
    36721 cgtcgcccaa atcactttgc cgccgagaat aaacagcagc agaccgccaa tgtttgacgt
    36781 tgcgttaagt aatttggcgt gagcagtggc tttggcgagg ttgaatccgc acagcgtaac
    36841 gaaggccagc gcgtaaaacg atccggcagc cgggccaaaa aatccatcgt aaaaaccgac
    36901 gcagcccccc gccaccagcg caaatggcag tccatacatc cgacgctggc gatcttcttc
    36961 acccagcttt ggcatcagca agaaatagag gccgatacaa atcaccagaa taggcaaaat
    37021 ctgccgtaag acatcggcct gaacatactg caccagtagc gcgccgctca ttgagccgac
    37081 aaaggtcatg gcgatattga gtttttgatc gcttaaacta accactttac ggcgaataaa
    37141 gtagatagtg gcggaaatag agccaccgca ggcttgcagt ttattggttg ccagcgcgtt
    37201 agcgggagac atccccgccg ccatcaatgc cggaatggtg agtaacccac cgccaccggc
    37261 aatcgagtcg ataaatcccg ccagcatggc gacaaaaaag agaactccca gcagcagcgg
    37321 ggaaaccata aacaggctat taaacgtttc cattagatca cgtgctcatc cagtagcgcc
    37381 tggcaggaag gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt tcccggtttt
    37441 ggaggttcaa accagctttg cagttctgcc ccgcaaccat cgcctggtgg cggtaaaggt
    37501 tgatcctcac actccagact atcggcagga caacgtaatc gtacatgcat atgcgcgcga
    37561 tgctggaacc agggtcgcac tttgcgcaac cagtcgcgat cggtgcccgc atcaaggcaa
    37621 agttgttgct taatcgccgg attaacaaaa atgcgcgtga cgtctttatc ctgggcggcg
    37681 agtttgatca agctgaaaat ttctggcttc cacagcgtcg ggacaacgtg tttaccgtcg
    37741 cgggaaacta aatctaacgc ttgcgggcgc aagagttgcg cggaggtcca gcgtgttttc
    37801 ggcagttgca ggaagatatc gacatccagt ccggtctggt ggctggcgtg accgccgttg
    37861 aaacgaccac cagcaggcat ccccatatcg ccaatcagca ccgtacccat gcccagattg
    37921 ctcacctggc gactcagacg ctggataaac atcaccaggt ctgggtgacc gaaatagcga
    37981 cgctgatcgg tacgcatgac ctgataatgt tcggactgta tcggcaacgt gtcagcaccg
    38041 atgatacagc cattagaaaa actgcctatc gattgtgcgc tacccggcac aggttgggtt
    38101 attttttgcc acggcgtcgc tgccaggctg gcgctactgg caagcagagc cagcagcgca
    38161 atcgcggttt tgttcatttt ttaccagcgt ggaatttcag tcttcacatc ggcattttgc
    38221 gcccgctgcc gtaacaggtg atccatcaaa acgatcgcca gcatcgcttc tgcgatcggc
    38281 accgcgcgga tcccaacaca aggatcgtga cggcctttgg tgatcatctc aacttcttcg
    38341 ccaaagcggt taatagtgcg ccccggcacg gtaatgctgg aggtcggttt cagcgccatg
    38401 tgggcaatga tttgctgccc gctgctgata ccaccaagaa tgccgcccgc atggttgctc
    38461 tggaaaccgt ctttggtgat ttcgtcgcgg ttctggctgc cacgcagcgc taccacgtca
    38521 aaaccatcac caatttccac gcctttcacc gcgttaatgc tcattagcgc atgggcgatg
    38581 tcggcatcca ggcggtcaaa gaccggctcg ccaagtccgg cgggtacgcc actggcaacg
    38641 acggtgactt tcgcaccaat agagtcgcct tcttttttca gcgcgcgcat cagttcatct
    38701 aacgcgtcga ttttgtccgg gtccgggcag aagaatggat tttgctcgac ctgagaccag
    38761 tctttgattt ccagcggaat gtcgcccatc tgggtcaggc aaccgcgaat ctcaatgcca
    38821 aatttctcgg cgagatattt tttggcaatc gcccctgctg caacgcgcat agcggtttca
    38881 cgggcggaag aacgtccacc gccgcgataa tcacgcaggc cgtatttttg ttcgtaagtg
    38941 taatcggcat gacctggacg gaaaacgtcc ttaatcgcac tataatcctg agaacgctgg
    39001 tcggtatttt cgatcaacaa tccgatgctg gtgccagtag taacaccttc aaaaacaccg
    39061 gagagaattt tgacctgatc cggctcgcgg cgctgggtgg tatagcgcga tgtcccaggg
    39121 cgacgacgat caaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca
    39181 ccatcgacga tgcagccgag cgccagcccg tgcgattcgc cgaaggtggt tacgcgaaag
    39241 agttgtccaa ttgtgtttcc agccatcacg gctccgttat tgttgtgttt gcgtgtttac
    39301 ttaatcttta taaatcgcga aatgttctcg tgcggcaata agctgctctt tagtgagcat
    39361 aaacacacca tcgccgccgt tatcaaactc cagccaggtg aacggaacat ccggatattg
    39421 ttccataaga tgtaccatgc tgttgccgac ttcacaaatc agcacgccat catcagcgag
    39481 gtaatctgcc gcgtttccga gaatgcgacg cgtcagtttc aggccgtcgg tgccagatgc
    39541 caggcccagt tccggctcgt ggcgatattc gtttggcagg tcggacatat cttccgcatc
    39601 gacatacggc gggttagtga caatcaggtc gtactgcact ttcggcaagt cgcggaacaa
    39661 atcggaccga ataggaatga cgttgtggat cagaccgtgt tcttcgatgt tctgttcagc
    39721 aaccgccagc gcgtcgggag agatgtccac cgcgtcgact tccgcttccg ggaaggcata
    39781 agcacaggca atggcgatgc agccgctacc ggtacacata tctaaaatgt gctgcggttg
    39841 cttgctgatg agtccggcaa atttattgtt gatcagttca ccaatcggcg agcgcggcac
    39901 cagcacgcgt tcatcgacgt aaaattcatg gccgcagaac caggctctgt tggtcaggta
    39961 agccaccgga atgcgttcgt tgacgcggcg gatcacgcgc tcaacaatac ggtgtttttc
    40021 gctggaggtc agacgcgcgg tgcgcatgtc ttccggaata tccagcggca ggtagagtga
    40081 aggcaacacc agttgcacgg cttcatccca cgggttatcg gtgccgtggc cgtaccagat
    40141 attcgccgcg ctgaagcggc tcaccgacca gcgcaacatg tcctgaatgg tttgcagctc
    40201 atttactgct tcatcaacga aaattttatc cacgtattcc tccagggcat gatcgcaata
    40261 atttcggcgg ctagtttgcc atgaagatga cgataaatca gcattcacgc gcggtgagtg
    40321 aggaaaaata cgtttaaaac gatcgattgc gctacgagtc gggtaaactg taggaaaatt
    40381 agaaatagag aaagataaat gaaaaagaaa acaacactta gcgaggagga ccaggcgctg
    40441 tttcgccagt tgatggcggg tactcgcaag attaagcagg acacgattgt ccaccgaccg
    40501 cagcgtaaaa aaatcagcga agtaccagta aagcggttga ttcaggagca ggccgatgcc
    40561 agccactatt tttcggacga gtttcaacca ttattaaata ctgaaggtcc ggtgaaatat
    40621 gttcgcccgg atgtcagcca ttttgaggcg aagaagttac gccgtggcga ttattcaccg
    40681 gagttgtttc ttgatttgca cggtctgacg cagctacaag ccaaacagga actgggggcg
    40741 ttgattgccg cctgccgccg tgaacatgtg ttttgtgcct gcgtgatgca tgggcacggg
    40801 aagcatattt tgaagcagca aacgccgctg tggctggcgc aacatccaca tgtgatggca
    40861 tttcaccagg caccaaaaga gtatggtggt gatgcggctt tgttggtgtt gattgaagtg
    40921 gaagagtggc tgccgccgga attgccctaa taaatattgc ggtataaacg tcttctccga
    40981 ccaggatact gaccggagaa gaattaaccc tacaactcat tcaaaatgta ctggaaacga
    41041 caggtggcat cgattggctg ctgactgctg gtgtcgatcc gtatttgcat atcatgcagt
    41101 tgcttatcgg taattggatg tgctaccggc gttactgtga ctttgctgtc gtcacacccc
    41161 gacgtgctga atgtgatcat atacgacata aagcgacaat aagcgccacg atcgtccatt
    41221 cggcttccat cacacacccg cccgtcgcgc atcaggtaag tcgagagcgt ttgtgttatg
    41281 actccccccg tcgattgcag cgttagcatc ttgttatgga ggacgtagtt ctcattccag
    41341 gagatatcat aggggccaac cggctccgtt gggcaccggg tttgttttgc tgatggacca
    41401 atgtaagtat aacctctcgc agcagtactc accgtgttgg tactgaccaa ccacgggcac
    41461 ccgtgagttt caccatgcac gatgatatcg acccccagat caacacatga ccatccatcc
    41521 gtaatccctg ggcaattgga gttatccaca gtattactct gatgagtgat gttcatcgtt
    41581 ggtgctgatc ccggtcccgt ccaaaccgtg ttagggatat tccccaacca caacgtatat
    41641 ccatcctgtg catattcgcc ataatagacc ccttccggcg tcaagacatc gatacgaaag
    41701 acaacttttg ttgtagcccc gttaccccaa aatccggtat aagtcttcac atccctgaat
    41761 gtgacgtccg cccaggcggt tgcactcagt agcccggtca gcagtaaaaa aaacagtccg
    41821 ctcatttttt tcatgttaaa tatactcaaa cgtcgcagtg agggaggccg taaagtcacc
    41881 catcgtcaca tcccgcccgc tttttgcctg tagccaggca ttaaaatgca gatcattgtt
    41941 gccgctgctc agcacaaatg tcgccccgct ggtattgttg attagcacag cagtgccgtc
    42001 ggtcttttcc atgccaatcc ccacaccttg tgctgtcgaa gcggcatcca gcgccagaaa
    42061 accgggctgc tcgctgtctt ccgttccggt aagtgtcacc cgaacctcat actgagcagg
    42121 ccctttgcaa tctttaagct gaaacaccac cggtacgcgc gcagattgcc cggtcaccat
    42181 cagatctcta cggctgaccg tggggaaatg cacttccgcc agcacctcgc cctgaaccac
    42241 cagcgtgcag gacttactga gtaaattgcc gtagaaatgc aggttgtctg ccgcctgaac
    42301 attcggcgtc agcagcaccg cggccagcgc agccgcgcag tgaaatatcc gttttaggct
    42361 cattggtaat ccacctgtaa tgttgcccag gcagaaaagg ttccttcgct gagtgaagca
    42421 tcactggctt tcaccggtac agcttgcagc gttggcggcg ttgacaggtt gattttcagc
    42481 ggcgtaccca acgtgaacgg ctggccattt tgttgcagct caatccccag cccggtgacg
    42541 tttgtctcaa tagccgcgtt atcgtagctg gtctggctgc ccgtccagct cagcgtcatc
    42601 tcccaggcat cgtcgcgcac gtcgggatcg catgtaatgg tatagggcac attctggcgg
    42661 aagttattgc cgttgatgtt gtcgataatg acgtcacgaa actcaacttc aacggttttc
    42721 ccgtcactga tggcacaggc gggcggcgaa accaatgtgc catgaaatgt gatatcgcta
    42781 tcggccgcct gtgcaatacc gcaataacaa ccgaataagc agagcgcaaa tcgtttcatt
    42841 gatactccac caccattgtc atggttgcgc taaactctga tgccgtaagc tgtgtgccgc
    42901 tttgtttcac cggcacggct tcgaaacccg ggcgcgaatt aacgttaaac ggcagccagt
    42961 tattacccgc caacgcaacc ggcgcatggc tggacgcatc ctgtatgcga atgccaaaac
    43021 cattgatgcc cgtactgagg accgtttcac cgttaatggt ggtggtggtc ccctgtaact
    43081 gcatctgtag gtcatcgctg acgctgttcg aacagttcag ggtgtaacca acaggctgcc
    43141 gatagttgct gccgtcgatg tttttaataa tgacattgcc aaactccacc gcgctgcctt
    43201 ttaccgtaca gggcggcggt gcatccacca gtatacgaag cgttaagttg accgttttac
    43261 tatcggccca ggcggagccg caggccagta acaccatgcc agcgacgggt aataaaactc
    43321 ctttcatgct ggatctcctg tcattagcct tttaggcttt gagcttcatc caccttgcag
    43381 gtgtctcctt cacaacggaa gaccagcggt aaacgtgcgc cgtagtcgtt gatgtaggtc
    43441 agcaccggag cgctgttcat ttttgccttt aacggttgtg acgttttcgg cggcatcacc
    43501 agtggtttaa agcccgcagc agttagcccc tttttctgcg tactggcatc gctgatgatg
    43561 acgtggtacg gcgtcgggtt attgacgata tagtcctggc cgctgcgcgt gagtgtgacc
    43621 ttaaactgcc acggttttct catgtcaatc ttctccagcg cctttggtcg ccagaacagc
    43681 ttgatgcggg tttgcagcgc aatttgcagg gtgttggctt tcccggactt tggcgggatc
    43741 tcacgcacat tgtagtagaa caggctttca cggtcagacg gcagcgtgtg aatgtccggc
    43801 agcgcctgaa tcttcacctg accgttcatc atggcgtcaa tacgctgcac cggcggcagt
    43861 accgccagcg gcgaagtgat tttgttgccc ttctcgtctt ccagccagct ttgcgccaga
    43921 taaggcagct tttcgttatt gttgcgcagg gttacgctta tcgatttatc actttcgtta
    43981 aacaccagac gggtgcgatc cggagtgact gatgcgtgcg ccgccaggtt ggtggtggtc
    44041 aacagtaccg ccagcgtcgt gctgaccacc attgaacagg ataatttata aaacattgat
    44101 tgttccttaa tcatattatt gagtggctga aattgacgtc ggcgcttcag atggcgtgac
    44161 ctgccgagtc tggtcctgaa taacgggctt aatttccggc gctaaagaat cgggggctgt
    44221 cgttcccttt tgttcacacg gcaacaacag gccgcggaac agatcggcag gtagcggctt
    44281 cggcaatacg atctcgcatt gtgcgctgcc ttcccagaac accatcatat gctcacccgc
    44341 attgatcccc gccagataga cattgccttc atcatccaca atgccaacct gctgctgctc
    44401 gtcgtttttt acttccgcgc caaacggcgg gtagctaccg tcacgcaacc gcagtaccgc
    44461 catcgctttt tgcccgctga tcactttgaa tttacgatag ccgattgcgc cttccgtcag
    44521 agtcgcctgc accaccgact gggtggcttc cgcatcttcc ggcaagttgt ttaaatctat
    44581 atacgcctga ttgcgatagt agttgttaat atcggcgacc accgccttgc caaacatatt
    44641 ggtgtacacc ggcgcgccgt tgctttcgac cggcacgttg gcaataccgt cggcgtcaat
    44701 cagcaggcgt gtgccgccca tgttttgagt gcgatgcagc gccccaccgt gcgcggtcag
    44761 cgttgccccg ccctgtaagg cgatccccgc cgaacggtat tctccttcat ggtagttggc
    44821 gctgagatct accttcgcca acgtgccgtc gtggctcaga taaccatcca cgctaccgtg
    44881 ctgttcgctg gtgccgacgt taatctggta gtgcgttgcg tcatcgatgc gattaaagta
    44941 gccgacctga ctgctgtccg acccgctacc gtaagagccg ttgtaggtca cggtgctgct
    45001 gtcgctccac ggaatgctca tgctgaggta cattccctta tccgtattat cgtcatattc
    45061 gtagcgatag ccggtcaccg agatgctcat attgcgaatg ctccccatat taaaatagtg
    45121 ggaaaacatc aggttatagt ttgtctgttc cgggcgatcc cagtaagtac gatgggagta
    45181 gttgagatag accgagacac ccgctgcggc aaagttctgg ttatacgtga tcgtatacat
    45241 ctctttgtcg tttccggtcc gcgccatgtc cgactggctc gcgtccagat actcgctcat
    45301 agtcataaag ttcttttcgg agaaacggta gcctgcaaag gtgacgcggc tgttaagttc
    45361 gtcaaagtct ttggcatagc tcacgcgaaa ggagttgccg tccagttttc ctttgccgta
    45421 tgcgctgtca tgatccaggt tgacgtgcga gtgagtcaca tcgaacgcca gcgcgccaaa
    45481 ctgtgcgagg tcgcgcccca cccccatcgc cgccgactgg tagtgctcat ctgccagcgc
    45541 gccaccgtaa agcgaccagc catctgccac cccccatgat gcttctccgc cggaaaaaaa
    45601 tccgccttcg gtcttgtggt tccagtcttc cggacgcccc atcatcactt tgtaacgcac
    45661 ctgcccctga cgcgtgagga atggcatgga tgcggtggtg acgtcatatt cctgcacctg
    45721 accattctgt tcttcaacac ggacgtgtag tgtgccagag acagaatcgc cgatatcctg
    45781 aatgcggaat ggcccggcgg gaacctgggt ttcgtaaagt acccggccca tctggctgat
    45841 ggtcactttt gcgctgctgt gcgccacgcc agaaacatct ggcgcatagc cgcgcagatt
    45901 gggcggcagc atctggtcat cggtgctgac actgctgccg atatagttaa agccgtcgaa
    45961 aatatcggaa ttgagataat cttctcccag cgacagcttc gcttttaaag agggtaaagc
    46021 tcgccaggcg taataacggc tccagtccca gtttttactc gttgtactgt tactgctgtc
    46081 atcttcatcg tcgttgctgc gggtgtgctg ataatcactt tgccagtctg cacggaaacg
    46141 ccatgcccca aggtttgccc caactgtacc gttaccgctg atatcatgtg agtcctcgcc
    46201 cccttgctcc tgatggcgag tttgcgcatt gaggctgtag tcggcaatca gcccagaaat
    46261 accatcgtcc cagcgtgagg gagggtccca gtcgctgctg gtatattcga ggtaagcctg
    46321 tggcactgtc agcagcaaag ccgactggct taaatcattc tcgacttcca tcccgtctaa
    46381 ttgccccggt ttaaggcatt catcgttgtg cgtccactgc aggctttttg ctatcccttc
    46441 tttcagcccc agcgccgcca ctaactcagg tgtcaggcag gcataagttt ttgttggatc
    46501 gttttcagag acataccaat taatgtcgta ttcatcagaa agggattgtt tatttataaa
    46561 tacacgtaaa ttatatttac ccgcgtcgac ataccctttt tttgaaaact taccgagatc
    46621 aattttcgtg tcgcccttta actcaaggaa acgggggtca aattgaatgg aatcatcagc
    46681 ccatgcattg actgatccac cagagattgc cagcgcaata tagcaggcga ttccccgcag
    46741 ccgaaaattt gagtgattag gcatacacat atccgtttgt gaaacagtcg taaatccgtt
    46801 gtgcgttgaa taaccccaca gcagaacgct gtggggttgt cacatcagag ataggtaatc
    46861 tggaatgtgg tcagggtttc gaactggccg agatccggcg catcggtttc acccaccagc
    46921 caggctttaa agttcagcgt ttgcttgtct ttacccttgc tagttgaagc atccatcacg
    46981 attggctgaa caatcttttc gcccacttta taacctttgc catgggtatc gccgattacc
    47041 aggctcacgt ggttcatggc aacaccgctg tcaacattga ataacgcata gttatctgcg
    47101 ttggctggcc ccgcgtagtt agtgccggtg aacgtggtct ccatgtttga ttcagtggta
    47161 aacacgcaat cctgtaaatg gatctggaaa tctttcggtg tggtagtgcc gccgccagtc
    47221 agtttagccg caccgatatc gcccatatcg acttccagtt tatgatcgtc tggaacgatg
    47281 gagcacgggg agtcttcaat agtgccgtaa aaacgtgcgg taccgttgtt acctgcggca
    47341 aaagcagaac tggagacgac tgcgcccatg accattgcgg cagcaatggc tgttttggc
//
LOCUS       DACADF010000016        47004 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.016, whole genome shotgun sequence.
ACCESSION   DACADF010000016 DACADF010000000
VERSION     DACADF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.016"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(4..96)
                     /locus_tag="ISB30_000912"
     tRNA            complement(4..96)
                     /locus_tag="ISB30_000912"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60..62),aa:Ser,seq:gct)
     gene            complement(412..597)
                     /gene="csrA"
                     /locus_tag="ISB30_000913"
     CDS             complement(412..597)
                     /gene="csrA"
                     /locus_tag="ISB30_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAP1988574.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(832..3462)
                     /gene="alaS"
                     /locus_tag="ISB30_000914"
     CDS             complement(832..3462)
                     /gene="alaS"
                     /locus_tag="ISB30_000914"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP1988575.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFDAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKSVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAVGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3591..4091)
                     /gene="recX"
                     /locus_tag="ISB30_000915"
     CDS             complement(3591..4091)
                     /gene="recX"
                     /locus_tag="ISB30_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP1988576.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARICQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4160..5221)
                     /gene="recA"
                     /locus_tag="ISB30_000916"
     CDS             complement(4160..5221)
                     /gene="recA"
                     /locus_tag="ISB30_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP1988577.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5301..5798)
                     /gene="pncC"
                     /locus_tag="ISB30_000917"
     CDS             complement(5301..5798)
                     /gene="pncC"
                     /locus_tag="ISB30_000917"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAP1988578.1"
                     /translation="MTESELMQLSEQVGLALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            5980..6219
                     /locus_tag="ISB30_000918"
     CDS             5980..6219
                     /locus_tag="ISB30_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988579.1"
                     /translation="MAIKKTDNTPQNVLKQTTQMRMWVIIGGISALISVICKVLSSGY
                     LEPFDFGQAALGVVCLGYAISLSKKIKQLRHSNKQ"
     gene            complement(6258..7343)
                     /gene="mltB"
                     /locus_tag="ISB30_000919"
     CDS             complement(6258..7343)
                     /gene="mltB"
                     /locus_tag="ISB30_000919"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAP1988580.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQSLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7600..8163
                     /gene="srlA"
                     /locus_tag="ISB30_000920"
                     /gene_synonym="gutA"
     CDS             7600..8163
                     /gene="srlA"
                     /locus_tag="ISB30_000920"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP1988581.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVRLAGATS"
     gene            8160..9119
                     /gene="srlE"
                     /locus_tag="ISB30_000921"
                     /gene_synonym="gutE"
     CDS             8160..9119
                     /gene="srlE"
                     /locus_tag="ISB30_000921"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP1988582.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGEATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9130..9501
                     /gene="srlB"
                     /locus_tag="ISB30_000922"
                     /gene_synonym="gutB"
     CDS             9130..9501
                     /gene="srlB"
                     /locus_tag="ISB30_000922"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP1988583.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9505..10284
                     /gene="srlD"
                     /locus_tag="ISB30_000923"
     CDS             9505..10284
                     /gene="srlD"
                     /locus_tag="ISB30_000923"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAP1988584.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10390..10749
                     /gene="gutM"
                     /locus_tag="ISB30_000924"
     CDS             10390..10749
                     /gene="gutM"
                     /locus_tag="ISB30_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAP1988585.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10816..11589
                     /gene="srlR"
                     /locus_tag="ISB30_000925"
                     /gene_synonym="gutR"
     CDS             10816..11589
                     /gene="srlR"
                     /locus_tag="ISB30_000925"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAP1988586.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11582..12547
                     /gene="gutQ"
                     /locus_tag="ISB30_000926"
     CDS             11582..12547
                     /gene="gutQ"
                     /locus_tag="ISB30_000926"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAP1988587.1"
                     /translation="MSEALLNAGRQTLMLELQEASHLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVDGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12544..14058)
                     /gene="norR"
                     /locus_tag="ISB30_000927"
     CDS             complement(12544..14058)
                     /gene="norR"
                     /locus_tag="ISB30_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAP1988588.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14245..15684
                     /gene="norV"
                     /locus_tag="ISB30_000928"
     CDS             14245..15684
                     /gene="norV"
                     /locus_tag="ISB30_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAP1988589.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15681..16814
                     /gene="norW"
                     /locus_tag="ISB30_000929"
     CDS             15681..16814
                     /gene="norW"
                     /locus_tag="ISB30_000929"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAP1988590.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDACIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGEPQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
     gene            complement(17036..19288)
                     /gene="hypF"
                     /locus_tag="ISB30_000930"
     CDS             complement(17036..19288)
                     /gene="hypF"
                     /locus_tag="ISB30_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAP1988591.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEEPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYCDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALAGGDLAAKQPWRNLLTQCLRFVPEWQSYPE
                     TVSVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LATSCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGGAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19441..19968)
                     /gene="hydN"
                     /locus_tag="ISB30_000931"
     CDS             complement(19441..19968)
                     /gene="hydN"
                     /locus_tag="ISB30_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAP1988592.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            20396..20833
                     /locus_tag="ISB30_000932"
     CDS             20396..20833
                     /locus_tag="ISB30_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988593.1"
                     /translation="MGSWGMEALESDEGLDLINWVEEQLQDDSTFDAESIVQRLSQHE
                     DLFGFQGDEEFLYDNYDNNVIGLVELIIQKAAGKKITSSKQIDQLDGYQLTSTFSKKL
                     QGRLQTIDDTHEWIMLFEGRAREKAKAYLIELTDKLRVVKTTA"
     gene            complement(20925..21344)
                     /locus_tag="ISB30_000933"
     CDS             complement(20925..21344)
                     /locus_tag="ISB30_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1988594.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(21370..21555)
                     /locus_tag="ISB30_000934"
     CDS             complement(21370..21555)
                     /locus_tag="ISB30_000934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988595.1"
                     /translation="MNIKTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGV"
     gene            complement(21580..22047)
                     /gene="hycI"
                     /locus_tag="ISB30_000935"
     CDS             complement(21580..22047)
                     /gene="hycI"
                     /locus_tag="ISB30_000935"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAP1988596.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEE"
     gene            complement(22040..22450)
                     /locus_tag="ISB30_000936"
     CDS             complement(22040..22450)
                     /locus_tag="ISB30_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAP1988597.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(22447..23214)
                     /gene="hycG"
                     /locus_tag="ISB30_000937"
     CDS             complement(22447..23214)
                     /gene="hycG"
                     /locus_tag="ISB30_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAP1988598.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPADILHPDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(23214..23756)
                     /gene="hycF"
                     /locus_tag="ISB30_000938"
     CDS             complement(23214..23756)
                     /gene="hycF"
                     /locus_tag="ISB30_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAP1988599.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(23766..25475)
                     /locus_tag="ISB30_000939"
     CDS             complement(23766..25475)
                     /locus_tag="ISB30_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAP1988600.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(25493..26416)
                     /gene="hycD"
                     /locus_tag="ISB30_000940"
     CDS             complement(25493..26416)
                     /gene="hycD"
                     /locus_tag="ISB30_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAP1988601.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(26419..28245)
                     /gene="hycC"
                     /locus_tag="ISB30_000941"
     CDS             complement(26419..28245)
                     /gene="hycC"
                     /locus_tag="ISB30_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAP1988602.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCSPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(28242..28853)
                     /locus_tag="ISB30_000942"
     CDS             complement(28242..28853)
                     /locus_tag="ISB30_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP1988603.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCATVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANTNTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(28978..29439)
                     /gene="hycA"
                     /locus_tag="ISB30_000943"
     CDS             complement(28978..29439)
                     /gene="hycA"
                     /locus_tag="ISB30_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAP1988604.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPVA"
     gene            29651..30001
                     /gene="hypA"
                     /locus_tag="ISB30_000944"
     CDS             29651..30001
                     /gene="hypA"
                     /locus_tag="ISB30_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAP1988605.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            30005..30877
                     /gene="hypB"
                     /locus_tag="ISB30_000945"
     CDS             30005..30877
                     /gene="hypB"
                     /locus_tag="ISB30_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAP1988606.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            30868..31140
                     /gene="hypC"
                     /locus_tag="ISB30_000946"
     CDS             30868..31140
                     /gene="hypC"
                     /locus_tag="ISB30_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAP1988607.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            31140..32261
                     /gene="hypD"
                     /locus_tag="ISB30_000947"
     CDS             31140..32261
                     /gene="hypD"
                     /locus_tag="ISB30_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAP1988608.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            32258..33268
                     /gene="hypE"
                     /locus_tag="ISB30_000948"
     CDS             32258..33268
                     /gene="hypE"
                     /locus_tag="ISB30_000948"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAP1988609.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLAPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            33342..35420
                     /gene="flhA"
                     /locus_tag="ISB30_000949"
     CDS             33342..35420
                     /gene="flhA"
                     /locus_tag="ISB30_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAP1988610.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            35465..35893
                     /locus_tag="ISB30_000950"
     CDS             35465..35893
                     /locus_tag="ISB30_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAP1988611.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(35932..36276)
                     /locus_tag="ISB30_000951"
     CDS             complement(35932..36276)
                     /locus_tag="ISB30_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAP1988612.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            36562..39123
                     /gene="mutS"
                     /locus_tag="ISB30_000952"
     CDS             36562..39123
                     /gene="mutS"
                     /locus_tag="ISB30_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP1988613.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            39229..39885
                     /gene="pphB"
                     /locus_tag="ISB30_000953"
     CDS             39229..39885
                     /gene="pphB"
                     /locus_tag="ISB30_000953"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAP1988614.1"
                     /translation="MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALEAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHLLPHIIEITNDNIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(39936..40733)
                     /locus_tag="ISB30_000954"
     CDS             complement(39936..40733)
                     /locus_tag="ISB30_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1988615.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRMNVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GRCIYLDAGTTTLAIAQHLIHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            40899..41807
                     /locus_tag="ISB30_000955"
     CDS             40899..41807
                     /locus_tag="ISB30_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP1988616.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IANADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            41804..43066
                     /locus_tag="ISB30_000956"
     CDS             41804..43066
                     /locus_tag="ISB30_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HAP1988617.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            43063..43701
                     /locus_tag="ISB30_000957"
     CDS             43063..43701
                     /locus_tag="ISB30_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP1988618.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDNPSKEVLFHLALYRNNPLCKAVVHLHST
                     WSTALSCLEGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            43706..44482
                     /locus_tag="ISB30_000958"
     CDS             43706..44482
                     /locus_tag="ISB30_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAP1988619.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRNYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTKEGLGWFDAWR"
     gene            44571..45935
                     /locus_tag="ISB30_000959"
     CDS             44571..45935
                     /locus_tag="ISB30_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HAP1988620.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(46078..46944)
                     /locus_tag="ISB30_000960"
     CDS             complement(46078..46944)
                     /locus_tag="ISB30_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP1988621.1"
                     /translation="MSQPYILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRGK
                     YLLQAIRDVTPLPVTALVYSHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQF
                     MNSRLPVATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLLN
                     PDQLPMMGFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLRD
                     TTIKVRKEESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPAN
                     IEMAIRLVGSYY"
BASE COUNT        11317 a        12659 c        12124 g        10904 t
ORIGIN      
        1 aaatggcggt gaggcgggga ttcgaacccc ggatgcagct tttgaccgca tactccctta
       61 gcaggggagc gccttcagcc tctcggccac ctcaccacac gcctcttacg agtgcttcga
      121 agaacttgtt tatcgctcat cgtcgctgcg tggcgcacat attactttct gagacttata
      181 agtcaaacaa tttttcccac acttttatcg tttgcacact tcacgctcaa ttagtctgta
      241 aaaacgtcaa aaagagtgtt ttatcaacag aaaaacggag gtctgacaga tagcagtaat
      301 gcaaaaaaat ggagacttaa gttgaatgaa cgggagtaaa gcgaaaagac aatggagtga
      361 aagagaaatc ttgagggtgc gtctcaccga taaagatgag acgcggaaag attagtaact
      421 ggactgctgg gatttttcag cctggatacg ctggtagatc tcttcacggt gaacagaaac
      481 ttccttcggg gcatttacgc caatacgtac ctggttgccc tttaccccta aaactgtcac
      541 ggtgacctca tccccaatca tgagggtctc accaactcga cgagtcagaa tcagcattct
      601 ttgctccttg aaagattaaa agagtcgggt ctctctgtat cccggcatta tccatcatat
      661 aacgccaaaa agtaagcaat gacaaacaca ttacatctaa gcagtcatgg cattacattc
      721 tgttaaacct aagtttagcc gatatacaca acttcaacct gactttatcg ttgtcgatag
      781 cgttaatgcg aatgccgtga agcgagtcca cggcattgcc tgacgcttat attattgcaa
      841 tttcgcgctg acccagcctt tcacactggc taacgctgca ggtaaggccg cagcatccgt
      901 accaccggct tgcgccatgt caggacgtcc accacccttg ccgcccacct gctgagcgac
      961 cataccaatc agttcccctg ctttcacacg atctgtgacg tccttagata cgcctgcaat
     1021 cagagaaacc ttaccttcgg ctaccgttgc cagcacgata attgtcgacc ccagctgatt
     1081 ttttaaatcg tcaaccatgg tacgcaacat tttcggctca acaccgctaa gctcgctaac
     1141 caacagctta acaccattaa catcaattgc cttactggaa agatttgcgc tctcctgtgc
     1201 ggcagcttgt tctttaagct gttgtaactc tttttccagc tgacgcgtac gttccagtac
     1261 tgaacgcact ttatcagcca gattattgct atcgcctttc agcagatgcg cgacttcgct
     1321 taagcgatcg ctgtctgcat gaacggtggc gatagcgcct tctccggtta ccgcttcgat
     1381 acgacgaacg cctgcagcag tacccgattc agagatgatg cggaacagac caatatcacc
     1441 agtgcggctg gcgtgagtac cgccacacaa ctcggtggag aaatcaccca tgctcagcac
     1501 gcgtacgcgc tcatcatact tctcgccaaa cagcgccatc gcacctttcg ctttcgccgc
     1561 ttcgagatcc atgatgttgg tttcgatcgg caagttacgg cgaatctgcg cgttcaccag
     1621 gtcttcgacc gcacgaatct cttctggttt catcgcttcg ttgtgtgaga agtcgaagcg
     1681 cagcactttg tcgttaacca gtgaaccttt ctgcgataca tgagtaccca gaacctggcg
     1741 cagcgcagcg tgcatcaggt gcgttgcgga gtgattcaga cgaatacggg cgcgacgagc
     1801 ctcatcaaca tcagcctgca cagcgtcgcc cactttcaga gaacccacag caagtttacc
     1861 gatgtgacca atcgcctggc cgtatttctg cgtatcttcc accgcgaagg agaagttagc
     1921 gcctttcagt tcgcctttat caccaacctg accgccggat tccgcataga atggcgtttg
     1981 atccagcacg accacagctt cctggcctgc attgatggca tcaaccgatt taccatcaac
     2041 aaacagcgca gtcactttgc cgttcagttc cagatggtca tagcctttaa attcagatgc
     2101 actgtcaaca cggatcattg cgttgtaatc tgcaccaaag ccgctggctt cgcgcgcacg
     2161 acgacgctgc tcttccattg ctgcgtcaaa accagcttcg tcaactttga tgttgcgctc
     2221 acggcaaacg tcagccgtca ggtcaaccgg gaagccgtag gtgtcgtaca gacggaaagc
     2281 agtttcacca tccagcgtat caccagaaag ttttgccagc tcttcatcca gcagcgccag
     2341 accgcgctcc agagtacggg caaactgctc ttcttcagtc ttcagcacct gctcaacctg
     2401 cgcctgctgg cgtttcaggt cttcacccgc agagcccata acgtcgatta gcggaccaac
     2461 cagtttgtag aagaaggttt ctttcgcgcc gagcatatta ccgtgacgca ctgcgcgacg
     2521 aatgatacga cgcagtacat aaccacggtt ttcattggac ggcattacgc catccgcgat
     2581 caggaacgca caagaacgaa tgtggtcagc gattacgcgc agcgatttat tgctcagatc
     2641 ggtcgcgcca gtgactttcg ctaccgcctg gatcaacgtg cggaacaggt cgatgtcata
     2701 gttagagtta acgtgttgca gcaccgcagc aatacgttcc agacccatac cggtatctac
     2761 agacggtttc ggcagcggtt ccatcgtgcc atcggcctgg cggttgaact gcatgaagac
     2821 gatgttccag atctcaatgt agcggtcgcc gtcttcttcc ggacttcccg gagggccgcc
     2881 ccaaatgtgg tcgccgtgat cgtagaagat ttcggtgcac gggccgcacg gaccagtgtc
     2941 acccatctgc cagaagttgt cagatgcgta tggcgcacct ttgttatcgc cgatgcgaat
     3001 aatacgttcg cgcgggatcc ctacttcttt ttcccagatt tcgtaggctt cgtcgtcgct
     3061 ttcatagacg gtaacccaca gacgctcttt cggcagggca aaccattttt cgctggtcag
     3121 cagttcccat gcaaactgaa tggcatcgtg tttgaaatag tcgccgaagc tgaagttgcc
     3181 cagcatttcg aagaaggtat ggtgacgcgc ggtgtaaccg acgttttcca gatcgttgtg
     3241 tttaccaccc gcacgcacgc agcgttggga agtggtagcg cgggaataat tacgcttgtc
     3301 gagcccaagg aacacatcct tgaactggtt catcccggcg ttggtaaaca acagagttgg
     3361 gtcgttatgg ggtaccaggg agctgctggc aactacctga tgtcccttac tatggaaaaa
     3421 gtcgagaaac gcctgacgga tctcagcggt gctcttgctc ataattatcc tgaaatcaag
     3481 ctaacgaaat atcgctacca gccgttgcgt gtcgttaacc gcccggctgg taactgaaaa
     3541 gtgggaataa gataagtttt cttgactggg aagtaaaatc ccgtatgcgt tcagtcggca
     3601 aaatttcgcc aaatatcctg gatatcttcc atcagatagc cacgatagag cagaaaacgc
     3661 tggatcttaa ctttttctga aaagacagtt ggcaaaggtt cgccatattt tcgcgtcgcc
     3721 tgatcgcgcg ccagtgcgca ccagtcgatg tcacattcac gcatcgcttt ttctgtcgct
     3781 tcgcgggaaa tacctttctg attcagttcc tgacaaatac gcgcaggtcc ataacctttg
     3841 cggctacggc tggcgataaa gcgcgcaaca aatcggctgt catcaagata gccatgttca
     3901 tggcaccagg caataacgcg ctcgtaatct tctgccgtgg catcaatctc ttccgggcca
     3961 tttttgccca taatcggtgc cgcgagttta cgtcgcagtt cttgctcact gtgatcgcgc
     4021 accgccagaa tgcgtaccgc acgatccaac aggcgagcat atgccgggcg acgggatgtt
     4081 gattctgtca tggcatatcc ttacaactta aaaaagcaaa agggccgcag atgcgaccct
     4141 tgtgtatcaa acaagacgat taaaaatctt cgttagtttc tgctacgcct tcgctgtcat
     4201 ctacagagaa atccggcgtt gagttcgggt tgctcagcag caactcacgt actttcttct
     4261 cgatctcttt cgcggtttct gggttatctt tcagccaggc agtcgcattc gctttaccct
     4321 gaccgatctt ctcacctttg tagctgtacc acgcgcctgc tttctcgata agcttctctt
     4381 tcacgcccag gtcaaccagc tcgccgtaga agttgatacc ttcgccgtag aggatctgga
     4441 attcagcctg tttaaacggc gcagcgattt tgttcttcac cactttcacg cgggtttcgc
     4501 tacccaccac gttttcgccc tctttcaccg cgccgatacg acggatgtcg agacgaacag
     4561 aggcgtagaa tttcagcgcg ttaccaccgg tagtggtttc cgggttaccg aacatcacac
     4621 caattttcat acggatctgg ttgatgaaga tcagcagcgt gttggactgc ttcaggttac
     4681 ccgccagctt acgcatcgcc tggctcatca tacgtgccgc aaggcccatg tgagagtcgc
     4741 cgatttcacc ttcgatttcc gctttcggcg tcagtgccgc cacggagtca acgacgataa
     4801 cgtctactgc accagaacgt gccagggcgt cacagatttc cagtgcctgc tcgccagtgt
     4861 ccggctggga gcacagcagg ttgtcgatat cgacgcccag tttacgtgcg tagattgggt
     4921 ccagcgcgtg ttcagcatcg ataaacgcac aggttttacc ttcgcgctgc gctgcggcga
     4981 tcacctgcaa cgtcagcgtg gttttaccgg aagattctgg tccgtagatt tcgacgatac
     5041 ggcccatcgg cagaccacct gccccaagcg cgatatccag tgaaagcgaa ccggtagaga
     5101 tggtttccac atccatggaa cggtcttcac ccaggcgcat gatggagcct ttaccaaatt
     5161 gtttctcaat ctggcccagt gctgccgcca acgctttctg tttgttttcg tcgatagcca
     5221 tttttactcc tgtcatgccg ggtaataccg gatagtcaat atgttctgtt gaagcaatta
     5281 tactgtatgc tcatacagta tcaagtattt tgtagaaatt gttgccacaa ggtctgcaat
     5341 gcatacgcag tagcctgacg acgcaccgca tcacggtcac cgctgaagca ttcccgccgg
     5401 gtaatgcctt caccacgggc agtggcaaaa gcaaaccaga cggtgccgac aggcttctct
     5461 tcactgccgc catccggccc ggcgatacca ctaatagaca cggcgtaatc agcacgagcc
     5521 gctttcagtg cgcctatcgc catttccacc acgacaggtt cgctcaccgc gccatgctgc
     5581 gccagcgtct cttcgcgtac accgatcatt tgcgcttttg cttcgttact gtaggtgaca
     5641 aatccgcgtt caaaccaggc ggaactgccg gcaatatcgg taatcacttt cgctacccaa
     5701 ccgccggtac aagactcagc agttgttacg gttgcgccac gggctttcag cgccagccca
     5761 acctgttcgc ttaactgcat cagttcactt tcagtcatca caacctctgt cagtcaaaaa
     5821 tttatgccgg acaagatagc actttcccgc aaaagatggg gacgagcgat tcattttacg
     5881 agtcgcgttc cgaaaaaaga ctgttgcggc cagtcggtag agaaaaagcg ctaccatgcc
     5941 gcgttcacat tgatatgtat tcacagggag agaatagcaa tggcaataaa gaaaacggat
     6001 aacaccccac aaaacgtgct caaacaaacc acacaaatgc gtatgtgggt catcattggc
     6061 ggcatcagcg cactgatttc cgtgatatgt aaagttttat cttctggcta tcttgaacct
     6121 tttgacttcg gtcaggctgc gttaggcgtt gtctgcctgg gatatgcgat atcgctgtcg
     6181 aaaaaaatca agcaattacg ccattcgaat aaacagtaag ataacagagg gaagtttaac
     6241 ctccctcttg caaatagtta ctgcacgcgc gccagcgcca cggcctgtcc taactgccag
     6301 accgccattg cgtaatgagt gctgtggtta taacgggtaa tagtgtagaa gttcggcaga
     6361 ccataccagt actggtaacc agtgccaaca tccagacgca gcaagctggc ctgctgatga
     6421 ttccccagcg actgctgcgg cgttagtcca gctgcggcaa gttgtgaaat gctgtacctg
     6481 gttttgaagc cattcggtaa gcctggcgcc tgaccgtttg ccatcaccgc cacctgatcg
     6541 cctttcaccc agccgtgcgc tttgaaatag ttcgctacac taccgatggc atcaaccgga
     6601 tcccacaggt tgatatgccc gtcaccgctg aaatccaccg catattgttt gtaagacgat
     6661 ggcataaact gaccgtaacc catcgcacct gcaaaggaac ctttcagatt gagcggatcg
     6721 tcctgctcgt cgcgcgccat cagcaggaag gtttccagct cacctgaaaa atattccgcg
     6781 cggcgagggt aattaaatga cagcgttgcc agcgcatcga gaatgcgagt tttccccatc
     6841 acgcgccccc agcgggtttc aacgccgata atcccgacga taatttccgg cggtacacca
     6901 tacacctgcc acgcgcgatt caacgcatct tcatactgat tccagaaaac cacaccgttc
     6961 tgcacgttgt ccggcgtaat aaattttttg cgataacgca gccatgcgcc gttcggacct
     7021 gatggcggtt tcaccgatgt ggttggtgcc tggttatcca tcagccgcag taccgaatcc
     7081 agacgtttcg cctgggagag aatttcctgc aactgctgac gatcgaaacc gtgtttgttc
     7141 accattttgt cgatgaactg ctgggcattc gggttattgg cgaagtcacc gcccatctgc
     7201 atcacattgt gctgcggctc aagcaggaag ccgccagacg gcgttccggt ggtcgtttca
     7261 gtctcagtag gttttggctt gctgctacag gcggcaagca acacaaaaag gggaagcaat
     7321 gttacataac gacgcttgaa catgaggggt ccatttaaca gattcaacca ggggcaagta
     7381 tggtaaagca tcccgccccg cacaaggaag cggtagtcac tgcccgatac ggactttaca
     7441 taactcaact cattctcctc actaccttct ttatacaaac tttcaaaata aaatatttat
     7501 ctttcatttt gcgatcaaaa taacactttt aaatctttca atctgattag attagattgc
     7561 cgtttggtaa taaaacaata aatactgaag gagagaacaa tgatagaaac cattactcat
     7621 ggcgcagagt ggttcatcgg gctgttccag aaaggcggag aagtgtttac cgggatggta
     7681 accggcattc ttccgctgtt aattagcctg ctggttatca tgaacgcatt gattaatttt
     7741 atcggtcagc atcgtattga acgatttgct caacgttgcg ccggtaaccc tgtttcccgt
     7801 tatctgctgc taccgtgcat tggtacgttt gtcttttgta acccgatgac cctaagcctg
     7861 ggtcgcttta tgccggaaaa gtacaaaccc agctattacg cggcggcctc ttatagctgc
     7921 cactcaatga atggcctctt cccccatatc aaccctggcg aactgtttgt ttatctcggc
     7981 attgccagcg gtctgacgac gctgaatctg ccacttggcc cactggcggt gagctatctg
     8041 ctggttggtc tggtcaccaa cttcttccgc ggctgggtaa ccgatctgac caccgccatt
     8101 ttcgagaaaa agatgggcat tcaacttgaa caaaaagttc gtctggcagg agcaacatca
     8161 tgacacgcat tcggatcgaa aaaggaacgg gtggctgggg cggcccgctt gagctggaag
     8221 ccacgccggg caaaaaaatc gtctatatca ccgccggtac ccggcctgcg attgttgaca
     8281 aactggcaca gcttactggc tggcaggcta ttgacggatt taaagaaggt gaacccgcgg
     8341 aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac attacgctgc ggcatctatc
     8401 cgaaacggcg tattcccacc attaatatcc actcgacggg caagtctggc ccgctggcgc
     8461 agtacattgt ggaagatatt tatgtctctg gcgtaaaaga agaaaacatc actgtagtgg
     8521 gtgaggcgac accacaaccc tcttccgtgg gccgtgacta tgacaccagc aagaaaatca
     8581 ccgaacaaag cgatggttta ctggcgaagg tgggaatggg tatgggttct gccgttgccg
     8641 tgttgtttca atctggtcgt gacaccatcg acactgtatt aaaaaccatt ctgccgttta
     8701 tggcgttcgt ctcggcgctt atcggcatca ttatggcttc cggtcttggc gactggattg
     8761 cccacggtct tgctccgctg gcgagccatc cactgggtct ggtcatgctg gcgctcatct
     8821 gctccttccc gctgctttca cctttcctcg gcccaggcgc agttatcgca caggttatcg
     8881 gcgtattgat tggcgtgcag attggtctcg gcaatattcc gccgcatctg gctttaccgg
     8941 cactgtttgc catcaacgcg caggcggcct gcgacttcat cccggtcggt ttgtcgctgg
     9001 cggaagctcg tcaggacacg gttcgcgtcg gtgtcccttc tgttctggtg agccgctttt
     9061 taaccggcgc gccaactgta ctgatcgcct ggtttgtctc cggttttatc tatcaataga
     9121 ggctgaaaca tgaccgttat ttatcagacc accatcaccc gtattggcgc gagtgccact
     9181 gacgccctca gcgaccagat gctcatcacc tttcgtgaag gcgcgccagc ggatctcgaa
     9241 gagtattgct tcattcattg ccacggcgag ttgaaaggtg cactccatcc cggtttgcaa
     9301 tttacgctcg ggcggcatcg ctatccggtg accgccgttg gcagcgtggc ggaagacaac
     9361 cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt taagcgaagc ggaatttccg
     9421 ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg cgccgggatc ggttttgaag
     9481 tttgaatctg ttaaggagta aaaaatgaat caggttgccg ttgtcatcgg tggtgggcaa
     9541 accttaggcg cgttcctgtg ccacggtctg gctgccgagg ggtatcgcgt ggcggttgtc
     9601 gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa ttaacgccga atatggtgaa
     9661 ggaacagcgt acggttttgg tgctgacgcc accagcgagc aaagcgttct ggcgctttct
     9721 cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg tctacagcgc cggaatagca
     9781 aaagcagcct ttatcagcga tttccagctt ggagattttg accgttcgct acaggtgaat
     9841 ctggtgggtt atttcctgtg tgcccgtgaa ttttcgcgtt tgatgattcg cgacgggatt
     9901 cagggtcgca ttattcagat caactcgaaa tccggcaaag tgggcagcaa acacaactct
     9961 ggctacagcg cagcgaaatt tggtggcgtc gggctgactc aatcactggc gctggatctg
    10021 gcggagtacg gcattacggt gcattcgctg atgctcggta acctgctgaa atcgcctatg
    10081 ttccagtcac tgttgccaca atacgcgacc aagctgggta tcaaaccgga gcaagtcgag
    10141 cagtattaca tcgataaagt accgctcaaa cgcggctgcg attatcagga tgtgctgaat
    10201 atgctgctgt tctacgccag tcctaaggcg tcgtactgca ctggtcagtc gatcaatgtc
    10261 accggcggtc aggtgatgtt ctgatcaaca gcggagatct tttaaggatc tccgcgcgac
    10321 taatagaatg cctgatgcgc tacgcttatc aggcctacag gacttccgcc actacatcac
    10381 ggagaagtta tggtatccgc actcatcacc gtcgccgtta tcgcctggtg tgcgcaactg
    10441 gccttaggcg gttggcaaat ttctcgtttt aaccgtgcct tcgacacact atgccaacaa
    10501 gggcgcgttg gcgtgggacg ttccagcggg cgctttaaac cacgggtcgt ggtcgccatc
    10561 gcgctggacg atcagcagcg catcgtcgac accttgttta tgaaaggact gaccgtcttc
    10621 gcccgaccgc aaaaaattcc cgcaattacc ggcatgcatg cgggtgattt acagcccgat
    10681 gtgatctttc cccatgatcc actatcacag aatgctctat cattggcgct taaactgaaa
    10741 cgtggataat ttcgttgtga atgttactag cttgcgaagt tatcattttg aaacctaaat
    10801 caggtaatca cgcccatgaa acctcgtcag cgtcaggccg ccattctgga gtatctgcaa
    10861 aagcagggta aatgctcggt tgaagaattg gcgcaatact ttgacaccac aggcacaacc
    10921 attcgcaaag atctggtcat tctggaacat gccggaaccg tcattcgtac ttatggcggc
    10981 gtggtgttga acaaagagga atccgatccg cctatcgatc ataaaacgct tatcaacacc
    11041 cacaagaaag agctgattgc agaagctgcc gttggtttta ttcatgatgg cgactcaata
    11101 attcttgatg ctggcagtac cgtgttgcag atggtgcctc tgctctcacg ttttaataac
    11161 atcacggtga tgaccaacag tctgcacatc gtcaatgcgc tatccgaact ggataacgaa
    11221 caaactatcc tgatgccagg cggaacattt cgtaaaaaat cggcctcatt tcacgggcaa
    11281 ctggcagaga atgccttcga gcatttcacc ttcgataaat tgtttatggg caccgacggc
    11341 atcgatctca atgcgggcgt aaccacgttc aacgaggttt ataccgtcag taaggcgatg
    11401 tgcaatgccg cgcgcgaagt gattttgatg gctgactcat cgaagtttgg tcgtaaaagc
    11461 cccaacgtgg tttgtagtct tgaaagcgtc gataagctga ttaccgacgc aggtatcgat
    11521 ccagcgtttc gtcaggcgct ggaagaaaaa gggatcgacg tgatcataac cggagagagc
    11581 aatgagtgaa gcactactga acgcgggacg tcagacgtta atgctggagt tgcaggaagc
    11641 aagtcattta ccggaacgtc tgggcgatga tttcgttcgc gccgccaata tcatcctgca
    11701 ctgcgaaggc aaagtggtgg tttcgggaat tggcaaatcg ggccacattg gtaagaaaat
    11761 cgccgcaacg cttgccagca ccggcacacc ggcttttttt gtccatccgg cagaagcact
    11821 gcacggcgat ctggggatga ttgaaagccg cgatgtgatg ctgtttatct cttattccgg
    11881 tggcgcgaag gaactggatc tcattattcc gcgtctggaa gacaaatcta tcgcgctgct
    11941 ggcaatgacc ggaaaaccga cgtcaccgct gggcctggcg gcgaaagcgg tgctggatat
    12001 ctccgtagaa cgcgaagcct gcccgatgca ccttgcgccg acctccagca ccgtgaatac
    12061 cctgatgatg ggcgacgcgc tggcgatggc agtcatgcag gcgcgtggtt ttaatgaaga
    12121 agattttgca cgctcccacc cagccggggc actgggcgct cgcttgctga acaaagtgca
    12181 tcatcttatg cgtcgtgacg atgccatccc gcaggtagcg ttaaccgcca gcgtgatgga
    12241 cgcgatgctg gaacttagcc gcaccggttt aggtctggtg gcagtgtgcg atgatcaacg
    12301 actggtgaaa ggcgtcttta ccgacggtga tttacgtcgc tggctggttg gcggcggcgc
    12361 actcaccacg ccagtcaatg aagcgatgac ggtcgacggc accacgttgc aatcgcaaag
    12421 ccgtgccatc gacgccaaag aaatcctgat gaaacgaaaa atcaccgccg caccggtagt
    12481 ggatgaaaac ggcaaactca ccggcgcaat taacctgcag gatttctatc aggccgggat
    12541 tatttaatcc ttcagcccca gacgtttcgc cagccgatgc aggttggcga cgtcggtttc
    12601 cagcatccgc gcacaggcgg cccagttgtg atgattttgc gccagtgcct gacgaatggt
    12661 ttcacgctgg aacgcttctg tcgcttcacg cagattttgc ttaacaacgg gcaccgccgc
    12721 cgcttctggc ggcggcaacg tcacctcagg aaaagcaaaa tgttgcgcct caagaatcac
    12781 ttcatcgccg ctgcgggtgg ctctcgacaa tactaccgcc cgatgaatag catgttccag
    12841 ttcgcgcacg tttcccggaa aattgtagtg ttgcagtaaa tttcgcgctc cggcacttaa
    12901 taccacgcgg gagagcccca gccgcaaacg acactgctcg cagaaatacc ccgccagcag
    12961 aatgacatca tcgccccgct cacgcagcgg cggcactgaa agtggaaaca cgctcaggcg
    13021 gtggaacaag tcagcgcgaa atcgccctgc cagtacctct tcgcgtaagt cacggttagt
    13081 cgccgccagc acgcgcacat cgacccgcaa actgcggtca tcgccaacgc gttgaatatc
    13141 gccatactgc aacaccctca gcagcttggc ctgcaatgcc aacgacaact cgccgatctc
    13201 atcgagaaac agcgtgccgt tatccgccat ttcaaacttc ccgctgcggt tactgatagc
    13261 accagtaaac gcccctttca catgcccgaa caactcactt tccgccacac tttccggcag
    13321 tgcggcacag ttgagataga ccagcggatt caccgcccgt ggcgaggctt catgaatcgc
    13381 tttcgccacc agctccttac cggttcccgt ctcaccgcta attaaaacgt tgagatcgga
    13441 tgccgccaca atctcaatct cttttttcaa ttgcgtcatg cctggggaca aaccaatcat
    13501 ctgcgtctgt ttcaccgctt caaacggcgc ggcatcgcct ggcagcatat tctggctttc
    13561 cagttgttca atcagtaacg cattgcttaa cgctcccgcc gccagcgcag caatcaggcg
    13621 taactcttcg tcgctgaaaa catcgaactg atcgggctgc atcccgtcga gcgtcaaggc
    13681 accgatcagg ttttgcccgg caaacaacgg caaaccaacg caggcgtgaa ccttcagact
    13741 ctcctgccct ggaatcagac cgtcataagg atcgggcaat tcgctgtctg cgggaaagcg
    13801 caccacatca cccgcgcggg caatcgcttc cagccgtgga tgcccttcca gcgcaaagcg
    13861 tctaccgagt acatccttcg ccagcccgtc gatggcaagc ggaataaact gccgcgagtc
    13921 gtaacgtagc aacgcagacg catcgcactc cagcacctga cgcagcgtag tgatcaggcg
    13981 ctgaaaacga tcctgatgac caatcccacg ctgcaattcg atggcgatat tcgccagcac
    14041 atcaacggaa aaactcatct ttgcctcact gtcaatttga ctatagatat tgtcatatcg
    14101 accatttgat tgatagtcat tttgactact cattaatgag cataatttta tttatagagt
    14161 aaaaacaatt agataaaaaa ctggcacgca atctgcaatc agcaagacat ctttttagaa
    14221 cacgctgaat aaattgaggt tgctatgtct attgtggtga aaaataacat tcattgggtt
    14281 ggtcaacgtg actgggaagt gcgtgatttt cacggtacag aatataaaac gctgcgcggc
    14341 agcagctaca acagctacct catccgcgaa gaaaaaaacg tgctgatcga caccgtcgac
    14401 cataaattca gccgcgaatt tgtgcagaac ctgcgtaatg aaatcgatct ggcggatatc
    14461 gattacatcg tgattaatca tgcagaagaa gaccacgccg gagcgctgac cgaactgatg
    14521 gcgcaaattc ccgatacgcc gatctactgt acagccaacg ctatcgactc gataaatggt
    14581 catcaccatc atccggagtg gaattttaat gtagtgaaaa ctggcgacac actggatatc
    14641 ggcaacggca aacaactcat ttttgtcgaa acgccaatgc tgcactggcc ggacagcatg
    14701 atgacttacc tgacaggcga cgcggtgctg ttcagtaacg atgctttcgg tcagcactac
    14761 tgcgacgagc atctgttcaa cgatgaagtg gatcagactg agcttttcga gcagtgccag
    14821 cgttactacg ccaatatcct gacgccgttc agccgcctgg taacgccgaa aattaccgag
    14881 atcctgggct ttaacttgcc agtcgatatg atagcgacct ctcacggcgt ggtatggcgc
    14941 gataacccga cgcaaattgt cgagctgtac ctgaaatggg cggcggatta tcaggaagac
    15001 agaatcacca ttttctacga caccatgtcg aataacaccc gcatgatggc tgacgctatc
    15061 gcccagggga ttgcggaaac cgacccacgc gtggcggtga aaattttcaa cgtcgcccga
    15121 agcgataaaa acgaaatcct gaccaatgtc ttccgctcaa aaggcgtact ggtcggcact
    15181 tctacgatga ataatgtgat gatgccgaaa atcgccgggc tggtggagga gatgaccgga
    15241 ttacgcttcc gtaacaaacg cgccagcgct ttcggctctc acggctggag tggcggtgcg
    15301 gtggatcgtc tttccacgcg cctgcaggat gcgggtttcg aaatgtcgct tagcctgaaa
    15361 gccaaatggc gaccagacca ggacgctctg gagttatgtc gtgaacacgg tcgcgaaatc
    15421 gcccgtcagt gggcgctcgc gccgctgcca cagagcacgg tgaatacggt ggttaaagaa
    15481 gaaacctctg ccgccacgac ggctgacctc ggcccacgga tgcaatgcag cgtctgccag
    15541 tggatttacg atccggcaaa aggcgagcca atgcaggacg ttgcgccagg aacgccgtgg
    15601 agtgaagtcc cggataactt cctgtgcccg gaatgctctc tcggcaaaga tgtctttgac
    15661 gaactggcat cggaggcaaa atgagcaacg gcattgtgat cattggttcg ggcttcgccg
    15721 cccgccaact ggtgaaaaat attcgcaaac aggacgcctg tattccatta accctgattg
    15781 ccgccgacag catggatgag tacaacaaac ctgacctcag ccatgttatc agtcaggggc
    15841 aacgtgccga cgaccttacc cgccagacgg caggtgaatt tgccgagcag tttaatctgc
    15901 gcctgtttcc gcacacctgg gtaacggata tcgatgccga agcccatgtg gtgaaaagtc
    15961 agaataatca gtggcaatac gacaagttag tgctggcaac cggtgccagc gcctttgtcc
    16021 cgccagtgcc cgggcgtgag ttaatgctga cgttaaatag tcagcaagag tatcgcgcct
    16081 gtgaaacaca actgcgggat gcccgacgcg tgttgattgt tggcggtggt ttgattggta
    16141 gcgaactggc gatggatttt tgtcgggcag gcaaagcggt cacgctaatc gacaacgctg
    16201 ccagtattct ggcgtcgtta atgccaccgg aagtcagcag ccgcttgcag catcggttga
    16261 cggagatggg cgttcatctg ctgttaaaat ctcagttgca aggactggaa aaaacagatt
    16321 ctggcattct ggcaacgctg gaccgccagc gctgcatcga agtggatgcg gtaattgccg
    16381 ccaccggact gcgcccggaa accgccctgg cacgacgcgc cgggctgacg attaatcgcg
    16441 gcgtttgcgt cgatagttat ctgcaaacca gtaatgccga tatttatgcg ctgggcgatt
    16501 gcgcggaaat taacggtcag gtattgccgt tcctccagcc gattcaactt agcgcgatgg
    16561 tgctggcaaa aaatcttctc ggcaataaca cgccgctgaa actcccggcg atgctggtga
    16621 aaatcaaaac gccggaatta ccgctgcatc tggcaggcga accccagcgt caggatttac
    16681 gctggcaaat taataccgaa cgccagggaa tggtggcgcg cggtgttgac gatgctgacc
    16741 agcttcgcgc ctttgtggtc agtgaggatc ggatgaaaga ggcatttgga ttgttgaaaa
    16801 cgttgtcgat gtaggcgagc tacagtgccc caaatgtcgg atgcggcgct gacgcgtctt
    16861 atccgaccta cagggaacgc atgtgttgat cacataatac gtttatgtcg cattgcactg
    16921 tcccaaaatg tcggatgcga cgctagcgcg tcttatccga cctgcgggga tgaatatgta
    16981 ggccggataa ggcgttcacg ccgcatccgg caatggtgtc caaatgcaac acgctttatc
    17041 cgttctggac ttcacccgct aaccaacgcg ccgcagcaat aaccccctgc cccagagaca
    17101 acccgccatc gcccgcaggt aaactctgtg gaaagagcaa tgtgaaatca gcgagataat
    17161 gcgccagacg tgcacgcagc aaacggttat gaataacccc gccgctaaat accagcgtag
    17221 tgataccacg catcgtggcc tgctcacgca tcaacgcggc ccccccctgc gccagcgcat
    17281 catgaaacgc ccacgcacgt tgattaaccg gagcctgcca gttcagccac tgctgccaga
    17341 aggtggcgag atccagttga ttatccacca gcggcatcgt caccggatgc gtcacgccgt
    17401 ggcacgaagt cgccagcgcc tccagagcac aagccgcttc accttcataa cttaacgtgg
    17461 ctggcgcaca gcccagcgcc gccgctacgg catcgaacaa ccgcccacac gatgacgcca
    17521 gtggtgcatt gattccacgc tcaatggccc gcgccagcac gctccagttt tgctgttgca
    17581 cacttaccgt ttcgggataa ctctgccact ccggcacaaa acgcaagcac tgcgtcagca
    17641 ggtttcgcca cggctgcttt gcggcaagat cgccaccagc aagcgccact gcaggcaagc
    17701 cgcccaggtg ctcgcattca cgatagttca cccgcaggca ctctcccccc cacaaagcgc
    17761 cgttctcccc cataccaata ccgtcgagtg tcaacgcaat gacatctccg ccatccagcg
    17821 gccagtgatg ttctgccaga catgccgctg cgtgagcatg atgatgcagc accgtttgcg
    17881 tcggcagatt catttcgcgc gcccactggc tggagacata gcccggatgc gcgtcatgca
    17941 caacgtattg cggagtgaaa tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact
    18001 gcatctggat gccatcgtca cttaaatcgc ccagatgctg actcaacacc gcttgttcgc
    18061 cgcgcaccag gcagaaggta tttttcagat ccgcgccgag acacagcaca ggcggaacat
    18121 ttttaaagcc cggaggcaaa gccagcgcat ccggcacata cccccgcgaa cggcgcagca
    18181 tttcgccgct ttcgcgcacc accgaatcat ccatccgctg cacgatgtcg cggttatgta
    18241 tcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg ctgatagccg
    18301 gtggtttacc gctcaggttg ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca
    18361 gcaaatgctg gagcgggttc gcaggcaaca ttaccccgac ttcgttaagg ccaggggcga
    18421 tatcatcaca aagctcaggt acatattttt tatccaccag cacaatcggc gcggcgggcg
    18481 tggtaagcaa ctggcgcgca gcgtctggta aaccgtcagc cactggcaac atgaccgcca
    18541 gcggtttcgc cgggcgatgt ttgcgcgccc gaagtgtcgc caccgcgtta ctgttacgtg
    18601 catcgcaggc aagatgaaat ccgccaatcc ctttgatggc gacaatgttg cccattttta
    18661 actgtgcgat agctgcctgt aatgccgcct cttgttctgc atgttcacca tgacttaccc
    18721 attcaagatg ggggccacac tccgggcagg ccaccggctg ggcgtggaag cgacgatcga
    18781 gcgggtcaca gtactctttg tcacaagccg gacacagcgg aaacgccgcc atcacggtaa
    18841 acgggcggtc gtaaggcatg gcgcgaataa tggtgaaacg cgggccacag tgggtacagt
    18901 tgataaacgg ataacgataa cgccgctcgc ccggggtatt catttcggca aggcaagcag
    18961 ggcaagtcgc ggcatcggga actatttgcg tattcatagc gccgcctgtg ctctggcgga
    19021 tagtgaactc ggtgggcagt tgtgaccaga taaacggctc acgctcgacg ctatcaatac
    19081 gcgccagcgg cggacaatgc tgatgcaatt gaacaagaaa ggtttccggc tcttccagca
    19141 gccggacttc tacgccatcg ccgtcattac agacatcgcc gtgaagattt aattgctgtg
    19201 ccagttgcca gacaaacgga cgaaaaccga cgccctgcac tttgccacga atacgcagtt
    19261 ggacaccgca agatgtgttt tttgccattg agttattccc gccatcatga attgcgtaac
    19321 ccgcccagcc ggacacgaca gcgtcgcatc cggcagtcac aggtcggcga tactgcccgc
    19381 ttcgtattct acgaatattt ccgggaattc ctttgatacc agaacagttc tgtaagattt
    19441 ttagaacatc agcgccgtgc ggcggcgttt ttctgcgctc agttgttcaa gtttattacg
    19501 atcgacacaa atcagcgcat gggtcgggca agccgccata cacgccgggc cgtcttcacg
    19561 atggttgcac aggtcgcatt tattggcttc ggctttgtca gcccgtacat tcagccctgc
    19621 gccgctgttg cgaatcaccg gacgcaccac cacttccatc gcaccatatg ggcaagccac
    19681 aacgcaggtt ttgcaaccaa tacaacgttc ctgcatgaca tgaacaaacc ctttatcacg
    19741 gctgatagca ccattcgggc agacgttagc gcacggtgca tcttcacact gacggcagac
    19801 tgtcgccgtg gaaatgttca cacctttaat gacatggatt cgcggtaaaa atgtttccgg
    19861 ggtcagcgat gcacagtcct gattttcctg atgagaaacc acgcacgcca cttcacaggt
    19921 acggcaacca atacatttac tcgcgtcagc aatgatgaaa cggttcatca aattctccag
    19981 caataacagt taatgcgccg atacattcac aaatcatgcc agtttttaat ttactgttat
    20041 ttaaggaaat taaattctgt actacaggaa aaacgatgtc atcgacacta gtgtcgatga
    20101 catgtgatga cagtatttat cgtagtaagg cagagagaat ccgttttcta atgaatattt
    20161 tatcgttgcc agtccacatc tgggtacttt tttccgacaa gtcgttctgg tggtataaaa
    20221 aagtaattgc cagtaacggg gaggatttaa gggaagcaac cgtgccggat acggcgtgaa
    20281 caacgaccac tttgtcagct atccatatga ttgtattttg aggttcatcg cagattagcg
    20341 atgaaccttt attgaataat actatttcga ctgaaataac aggtaaggat acaaaatggg
    20401 ttcatgggga atggaggctc tcgaaagcga cgaagggctg gatttgatta attgggtaga
    20461 ggaacaactg caagatgatt cgacctttga tgccgagtca attgttcaac gcttaagcca
    20521 gcatgaagat ttgttcggat ttcagggaga tgaagaattt ctctacgata attacgataa
    20581 taatgttatc ggtctcgttg aacttatcat tcaaaaagca gccgggaaaa aaatcacatc
    20641 gagtaaacaa attgatcaac tcgacggtta tcagctcacg tcgactttca gcaaaaaact
    20701 ccagggccgt ttacaaacaa tcgacgacac acatgagtgg ataatgcttt tcgaggggcg
    20761 agcacgcgaa aaagcaaaag catatctgat tgagcttact gataaattaa gagtagtaaa
    20821 gacaacagcc tgaatgacaa acaagggcgc gcaaaattaa gaatctgcgc cctttcaaag
    20881 cccgataggc gtagcgcatc aggcgattta gcgtctgcca tcagtcaaag aaaccattcc
    20941 ggcttaacgc caaatcgggc tgaaagtgcc ttaatgtggg atatcgtcag ggaacgttgt
    21001 ccagataaaa tctggctgac cagggattta gagccgattt cgttcttcaa atctgcatag
    21061 gtcagattat gttgatcaat cagcgtgcga agtaacgcca caccaacagg cattgctgca
    21121 acggctttgt cgaactctgc aaacttttcg ttattgtttt catattctgc aatgcggctc
    21181 gccagaaagt cgataagtgg attagtatca tcttcttcaa tcaaatattc gaccagttcc
    21241 agtgcttcac ggtaatcatc ttctgagtca ctgcctccca gaaaggggac tgcattgacc
    21301 agttctcttg ttgctttaac agcgcgtgca gcattagcag tcattctttg ttctctctgt
    21361 agtaacgtgt cagacgccca cttttctaaa atgccaggtt tgctcggcat cataaggcat
    21421 ggtcaggaga ggtttcagcg cccgcttttg cgcgtattcg ctgtcactca ttggcagata
    21481 ttcggtaaag ctggtggcct gtgcaggcag cactaagctc aataaaaaag cgcacatccc
    21541 tgttttgatg ttcaattccc tgaattccct aatgaagaat tattcttcca ccgctaactg
    21601 cgcgaagccg ccatttcctt cccagccttc cagccgctga tacaccgttt ccaccgcatc
    21661 tttaatcggc tgagtcatcg ggtagtaaaa gccgacgata tccggctgga tgccaaggaa
    21721 aatcacttcg ccaatatctt ctttcaactg gtcgataaga tagttcagcg gcatgttatg
    21781 ggtagtcatc ataaacatct cggcgatgtc agccgggtcg atgatgcgga tctcgccagg
    21841 gtttagcccc atatccgtgg cgtcgacaat cagcagccgt gtcgggcgca gttcgcggat
    21901 agcgacaatg tcgttttccg gtgcgctacc gccgtcaatc actacccagt tacctttcgg
    21961 cgcggcggcg cacttttccg ccagtagcgg gcctgcgcca tcatcgccca tcatgctatt
    22021 gccaacacaa agtaaaacgt cagtcacgca gtctcctcac catcaaatag atggcgtttt
    22081 cctgatgaat atcatgcagc atacttaaca gcattgtact ccattccttc tgctgcgaag
    22141 tttgcgctgc ccgggcttta tcgaatgcgt tggccagcat cggcacatgg ttaatgtcga
    22201 tgacgatctc accatatttc ggcacgcctt ccattttacg gcgggcttcg ctgcccgcct
    22261 ccagagtggc aatccatgcc agatagtcat cccacgggca ggtaagtgcc gcttccaggc
    22321 aatcgataac cccaaggtgg tgaccaatcg ccaggctgta atagaccacc tgctgcgcct
    22381 cggcgggcgt ggcatcgttc tcatcaataa atttacggct cagttgactg aacaccacct
    22441 tttcactcat cggatacgcg cctcttcaac aacatgattc agatggctga caatctcgtt
    22501 cagacgcgga tcgttttccg cttccagcca gcgtgcaacc tgttcttcgc cctgccctaa
    22561 ctgtgtcagg aaatcatcgg caatctggcg accgtaacga taacccgcca gacgacgcgc
    22621 ttcacggtcc actttcacac gcaacggttg caccatatca ggatgtagaa tgtctgccgg
    22681 ctgctcatct tgctcacccg gcccacgggc gtggattttc tgctccagca ggccgagcgc
    22741 catcgcaaag ccgtacagcg tggcggcagg cgttggcggg cagccaggga tgtagacatc
    22801 taccggaacg attttatccg taccgcccca cacgcagtag agatcgtgga agatcccgcc
    22861 gctgttaccg caggcaccgt aagagataca gattttcggg tccggcgcgg actgccacgc
    22921 acgcagcgca ggggatcgca ttgcacgggt gaccgcgccg gtaaacagta aaatatccgc
    22981 atgacgcggt gacggaacga ctttaatgcc gaagcgttct gcatcgaaca gcggcgaaag
    23041 cgtggcgaaa atttcgattt cgcaaccgtt acagccgccg cagtccacgc gataaacgta
    23101 ggcagaacgt ttgatttttt tcagtaacga cgccttcatg ctggcgatgg attcatccac
    23161 cgtcatcggg accggaatgc cgttggcgtc acgggggcct aataaattgc tcatcagatg
    23221 gcctctttca tatggcgagt cagttcaata cggtcggacg gcaccaggca tttctggcgc
    23281 ttacattccg ggcaagtctc aaagctttcg cggtggtttt ccgcgcggct gtcgccgttg
    23341 tgcttaagca gcgcaatggc gtagtcgatc tctttctgga cggcgaaagg acgattgcag
    23401 acgcggcagt tgcacagcgc gaagcgggac tgttgcagga agtcttcttt cttccacacc
    23461 gccagttcgt attcttgcga cagcttaatc gccgccgtcg ggcagacttc ttcgcagcgt
    23521 ccacagaaga tgcagcgtcc aagattgaac tgccacgcca gctctcctgt ggcgaggtca
    23581 gtttcaaccg ttaaggcgtt tgacgggcag gcattgacgc aggccgcaca gccgatgcac
    23641 tgctgcgggt tctgctctgg cttaccacgg aagtttttat caaccgcaat cggctccagc
    23701 ggataagacg aagtcgccgt gccggttttg atgacttttt tgataaaggt aaacatggcg
    23761 attccttatt tcagcggcga gtttttacgc tcaatgctgt agcgctcgag ttctttgtac
    23821 ggcaccactt tgctcttctt cttacgtaca tcgaccacgg tcatgcggtc ggtacaggag
    23881 tagcaagggt ccaggctacc gataatcagc ggcgcatcgg aaacggtatt gccacgcagc
    23941 atgtagcgca gggtcggcca gttcgcgtag gtcgcggcac ggcagcgcca gcggtacagc
    24001 ttctggttgt cgccggtcat gctccagtgg atatcgtcgc cgcgcggcgc ttcggcaaag
    24061 cccagcgcga aacggtgcgg aatgtaggta aagccttcca ccatcagcgg gccgcccggc
    24121 aggttatcca gaccgtaatc gatcatattc agcgcggtat agacttcgtt gatacgcact
    24181 ttcagacggg aaataacgtc gcagccctgc tcgctgtgga cttccattgg cagcaggcca
    24241 taaccaacaa acgggtgatc ggcgcgggta tcgcgagcgt gtccgctggc gcggaccatc
    24301 gggccaacgt tactgaaatc gcgggcaatt tccgggtcca gacgaccaat gccgacggtg
    24361 cgctgttcca tgttcggtgt gctcagcagc acatccacca gctcctgcac ttcacgacgc
    24421 atctgttgcg ccagctggcg ggtctgaatc atgtcgtctt tcagcagatc gcgacgaatc
    24481 ccgccgatca ggttcaggcc gtaggtttta cgcgcaccgg taaggatctc tgccattttc
    24541 atggaggttt cacgcacgcg gaagaactgc ataaagccgg agtcaaagcc ggtaaagtga
    24601 caggccaggc cgaggttgag cagatgcgag tgcaggcgtt ctacctccag cagaatggcg
    24661 cggatcatct gcgcgcgttc tggcacctga atacccatcg cgttttccac cgacgtggta
    24721 taggcggtgc tgtgagcaaa gccgcagatc ccgcacacac ggtcagagag gaaggtcact
    24781 tcgttgtaac ccatacgggt ttccgccagt ttttccatgc cgcgatggac gtagaacagg
    24841 cggtagtcgg cgtcgataat gttttcacca tcgacgaaca gacggaagtg acccggttcg
    24901 tcagaagtga cgtgcagtgg accaatcggc acgacgttgt ttttcttgtc gcccagttcg
    24961 ttgatgaact cgtaggtttc agcatcggta gtcggtgccg gacgctgacg ataatccatg
    25021 ctgtctttac gcagaggata aagttcatcc ggccagtcat ccggcagcac cagacggcgt
    25081 tcatccggca gaccaaccgg aatcaaaccg tacatatcgc gcacttcacg ctcgccccac
    25141 accgccgccg gaacgcgcgg cgtcacggaa ggatactccg gtttgttggc gtcaacttca
    25201 acgcgaaccg ttacccaaca cttggtgccc ttctccatcg acagcacgta ataaacggcg
    25261 taatgaccat tcagtttgcg ttcgtcgtta ccaaacagca ccgacagcca gccaccctgc
    25321 ttgtagtaga gaaactccac cacttccggc aggtagttca cctttacggt cacggtcagc
    25381 tgatctttgg tctgccaggc gtggtccagc acgacgccag gaaatgcctc attcagcgcg
    25441 gcgagataat gttgacctaa tttttcttca gacatgctca aactctcttt aatcacgccg
    25501 ccagcaagga gacgaacgct aaaaatgcaa agccaaaccc ggcccaggta atgcgcggag
    25561 taatatcaag acgcagacgg gccatgctgt tttcgaacag cgcgataacc aggacgccaa
    25621 ccaccagttt gacgatggca atcaccagcg ccagcagcag cccaccgacg gtgaaggttt
    25681 ccatttgtcc ccacggaata aacaccccga cgaacatctg caacaccacc agctgtttca
    25741 ggctgatacc ccatttcatg acgccaaagc cgctgccgct atactcagag agcggacctt
    25801 cctgcaactc ctgctcggct tccgccaggt cgaacggcag tttgcccatt tcgataaagg
    25861 tggcgaatgc acaggcacaa agcgccagca ccagcgggat gctctggctc agtggccagt
    25921 gataaacggt gtcggtgatg ttgctgatgt tggtggaacc ggcaacctgt gcggcgaccc
    25981 acagaccaag cagcagcatc ggttcaacca acacgccaag catcgcttca cggctcgcgc
    26041 cgatagcggt aaacgggcta ccggtatcca gaccagaaat ggcaaagaag aaacgcgcga
    26101 tggcgaagag atacagtaag gtgatcaaat cgcccagttg cggcagcgga gaaccgacgg
    26161 tcaccaccgg cagcgcagtg gcgatagtca gcatgacgcc caccatcaca tacggcgtca
    26221 ggcggaacac ccagccggag gcatccgggc caacgctctg gcggcccagc agtttgataa
    26281 tgtcgcgata ctcctgcaac acacccggcc cacgacggtt atgcagacgg gcgcgtgcta
    26341 cgcgggtaat accggagagc agcggcgcaa cggcaaatag caccagcgcc tgaattaacg
    26401 gatataaaac actcattctc aggctcctcg tgaaacaata atcaccacca gcaccgccag
    26461 ttcaaccagc gccatccggc ggaacagcaa cgcactcccc tcgcactgcc agcccggcac
    26521 cagagacacc ggattcagcc atttgcgtag tttcagcacc ggcgcaaacg cctgtttcac
    26581 cggcatggca aaaccgtgag cggtaatcac cattgatttt tcatgatcgt agccgcacac
    26641 ccaggccgcg ccgcgggaac gcgacggcaa acgatcgcct ttgcaaatcg ccataatgat
    26701 gaatggcagc agcgggcagg caatcaacaa caacgtgatc attggttgag aaacggtggt
    26761 gttagcaggc tccagcggca gaggtacagc agcagagagc atcggcagta gccacggcgc
    26821 agcaacaccg ccaattacgc agcaaatcgc cagtgccact acgcttacgc tcatcaggag
    26881 cggcgaacag gtggcgtttt cggcttcttt ggtacgcggc gcgccgagga aagtaacgcc
    26941 ataaactttc gccatacaca tcaccgccag cgcaccggta attgccagcc ccacggcgag
    27001 cagcggcccg agaagacggg caacaaacgc gccactattg ctcagtttga agaaggattg
    27061 atagataacc cattccccgg caaaaccgtt cagcggcggc agcgcagcca ttgccatcag
    27121 cccgactaac atggcgatgg agataaccgg cattttcttg ccaataccac cgagtttttc
    27181 gatatcgcga tgaccggtac ggaaccagac gctccccgca ccaaggaaca gtacgctctt
    27241 gaacaggcta tggttaagca gatggtacag accaccaacc agaccaaggg caatcagcgc
    27301 cggttgttcg agcgcgatac ccgttacgcc agcgcccagc cccagcagga tgatgccgat
    27361 attttccagg gtgtggtaag ccagcagacg ttggatatta tgttccatca acgcatacag
    27421 accgccgacg aatgcggtga tcatgccgag taccagcagt gcgatccccc accacagcgg
    27481 tgcattaccg cccagtagtg acagagttaa aatgcccagc aggccaattt tcatgactac
    27541 cgtggaaaac agcgcggcag ctggcgcaga ggcgttggca tgtgcctgtg gcacccagcc
    27601 gtgcagcgga ataatcccgg ccagcaggcc aaagccaatc acaccgagca gccagatatc
    27661 ggaaccgagc ggcagctgtt gcatacgcat atccagcagg cgcagatcca gcgtgccgta
    27721 acgctgccac agcagccagc aggcaatcgc cagcagcaga gtgccaagac gccccagcgc
    27781 aaaccacagt ttgccctctt tgctgttgct ggtgaggaac accgcacaca gggccatgat
    27841 ttcggccatt accacgaaca tgccgaggtt gctggcgatg acggcacaga cggcggcagc
    27901 catcaacata ttgatctgca agccgttgca cttcacctgc gcgtggcgat gccagtcaat
    27961 gttgtagagg ctgacaaaca gaccgcacag accgagcgta atcagccaaa tcgcgttaag
    28021 cggagagatt tgcacatcgt agcttaccaa cgacagcgca ccgctcacgc caaccgcgcc
    28081 agtcagcaca gtgaagcccg cggctgccgt atacaggcta cccaccgcgc cgccaattcc
    28141 ggctatccag ccacttagcg ctttttgaaa agaaaagaga aatgccagaa cagcggcggc
    28201 gacaaaccac gccacgccgc tattgatcag ggaaattgcg ctcatttagc ctctccactt
    28261 tgagcctgct gaaacaaggt gagatcgcca aagtccgtgt taaaagtcag ctcacgctta
    28321 cgtttgctga cgcgggcgat atcggtgtta tccaccagat gcagggcttt agtcgggcac
    28381 atccgcacgc aggccggacc ttgttcatca aagctacaaa ggtcacattt gacggcgatc
    28441 gcgcgaatac ctggcaccca gtcaagcaat gtgctgacac gcgccggagc aggcggtgcc
    28501 ggtggcgctt tcggggtatt ggtgtttgcc ggaatatcca gcggacggct gccggaaaat
    28561 tcaattgcgc caaacgggca ggcgatgcca cacagtttgc agcttacgca caagctttca
    28621 ttcagctgta cggcaccatc aacacgagtg atggcgttaa ccgggcagac cgttgcgcag
    28681 ggtgcatctt cacagtggtg acagagctgc ggcgcagatt ctttttcatt gagcatcact
    28741 cgcaggcgcg gcattgattg caggccgtgc tggcgatgcg tctctgaaca ggcagcctca
    28801 caagtgtggc agccgataca gagcgtggag tcagcaatta caaaacgatt caccaggcat
    28861 tcctcaggtg attgtcattt ttgacgaaaa catgccgatg aaatgtcatt ttcgacactc
    28921 atcgacacgc ccatccccaa acaggcgtaa cgcctgcaaa acgggcaaag cctcagctca
    28981 tgctaccggg ctttgtccct ttaccagttg gcttaaattc accggcacat tgttttcaat
    29041 ggcggtatat aagctgtcgt aaacgccagc gattttttcg agatagtgtt ccagaacttg
    29101 ttcgcgatcg cggatgttga ccttgccatc aatcatgccg tcaatgctca actgcgcctg
    29161 cgcaatccgc tgtttgtctt cgccatcttt tgtttcgacg taatactcga tggtgtggct
    29221 gttaaagcca tccgccaggg tgacgtaaat gcgaaaatgt tcaaaaagga cgaaacagag
    29281 ttctttgtcc ggcgcgcagc tcatggcgtg atgcaggcgc gctttcgata acgtgatccc
    29341 ctgaaccagc gaattgcagt agatgtgcca ctggtcctgt aggcgacgat gccgctgtgc
    29401 gatgtaatcg gctttctcgc ttatttccca aatagtcatt gtcaggttac ccgtttaaca
    29461 gagatgccag ctttaagcat tttctgtgcc aacttttaat tcattgttat taaagcgatt
    29521 tctaagctaa agatgaattt cgtcgccgtg tcgacgtgtc atttcgacat catcgacatt
    29581 attcaccgca gggataatca acactggcac aattattgct tgtagctggc aatagttaat
    29641 gggaggcgat atgcacgaaa taaccctctg ccaacgggca ctggaattga tcgaacagca
    29701 ggccgcaaaa cacggcgcaa aacgcgtaac tggggtctgg ctcaaaattg gcgcattttc
    29761 ttgtgtcgaa accagctctc ttgccttttg ttttgatctg gtttgccgcg gcagcgtggc
    29821 ggaaggttgt aaactgcacc tcgaagaaca agaggccgaa tgctggtgtg aaacatgcca
    29881 acagtatgtg acgctactga cccagcgcgt ccgccgctgt ccacagtgtc atggtgacat
    29941 gctacagatt gtggcagacg acggtttaca gattcggcgg atagaaatag accaggagtg
    30001 agcgatgtgt acaacatgcg gttgcggtga aggcaacctc tatatcgagg gtgatgaaca
    30061 taaccctcat tccgcgtttc gtagcgcgcc atttgccccg gcggcacgcc cgaagatgaa
    30121 aatcaccggc attaaagcgc ctgaatttac ccccagccag actgaagaag gcgatctgca
    30181 ttacggtcat ggcgaagcgg gcactcacgc gccgggcatg agccagcgtc ggatgctgga
    30241 agtcgaaatt gacgtgctgg acaaaaataa ccgtctggct gaacgcaacc gcgcgcgctt
    30301 tgctgcccgc aagcaactgg tgctcaacct ggtttccagc cctggttccg gtaaaaccac
    30361 cctgctgacg gaaactttaa tgcgcctgaa agacagcgtt ccgtgcgcgg ttattgaagg
    30421 tgaccagcaa accgtgaacg atgccgcacg cattcgcgct accggcaccc cagcgattca
    30481 ggtgaacacc ggtaaaggct gccatcttga cgcacagatg attgccgacg ccgcgccgcg
    30541 cctgccactg gacgataacg gtattctgtt tatcgaaaac gtcggtaacc tcgtttgccc
    30601 ggccagcttc gatctcggtg aaaaacacaa agtggcggtg ctttccgtta ccgaaggtga
    30661 agacaaaccg ctgaaatatc cgcacatgtt tgccgccgcc tcgctgatgc tgctcaacaa
    30721 agttgacttg ctgccgtatc tcaactttga cgttgagaag tgcatcgcct gcgcccgtga
    30781 agtcaatcca gaaattgaaa tcatccttat ttccgccacc agcggcgaag ggatggacca
    30841 gtggctgaac tggctggaga cacagcgatg tgcataggcg ttcccggcca gatccgcacc
    30901 attgacggca accaggcgaa agtcgacgtc tgcggcattc aacgcgatgt cgatttaacg
    30961 ttagtcggca gctgcgatga aaacggtcag ccacgcgtgg gccagtgggt actggttcac
    31021 gttggctttg ccatgagcgt aattaatgaa gccgaagcac gcgacactct cgacgcctta
    31081 caaaacatgt ttgacgttga gccagatgtc ggcgcgctgt tgtatggcga ggaaaaataa
    31141 tgcgttttgt tgatgaatat cgcgcgccgg aacaggtaat gcagttaatt gagcatctgc
    31201 gcgaacgtgc ttcacatctc tcttacaccg ccgaacgccc tctgcggatt atggaagtgt
    31261 gtggcggtca tacccacgcc atttttaaat tcggcctcga ccagttgcta ccggaaaacg
    31321 ttgagtttat ccacggtccg ggttgcccgg tgtgcgtact gccaatgggt agaatcgaca
    31381 cctgcgtgga gattgccagc catccggaag tcatcttctg tacctttggc gatgccatgc
    31441 gcgtaccagg gaaacagggg tcgctgttgc aggcaaaagc acgcggtgcc gatgtgcgca
    31501 tcgtttactc gccaatggat gcgttgaaac tggcgcagga gaatccaacc cgtaaagtgg
    31561 tattcttcgg cttaggtttt gaaaccacca tgccgaccac cgccatcact ctgcaacagg
    31621 cgaaagcccg cgatgtgcag aatttttact tcttctgcca gcacattacg cttatcccga
    31681 cactgcgcag tttgctggaa cagccagata acggtatcga cgcgttcctc gcgccgggcc
    31741 acgtcagtat ggttatcggc actgatgcct ataattttat cgccagcgat tttcatcgtc
    31801 cgctagtggt cgctggtttc gaaccccttg atctacttca aggcgtggtg atgctggtgg
    31861 agcagaaaat agcggcccac agcaaggtag agaatcagta tcgtcgggtg gtaccggatg
    31921 ccggtaacct gctggcgcaa caggccattg ctgatgtgtt ctgtgtcaac ggcgacagtg
    31981 aatggcgcgg cttaggcgtg attgaatctt ctggcgtgca cctgacgccg gattatcaac
    32041 gattcgatgc cgaagcacat ttccgcccgg caccgcagca ggtctgcgat gacccgcgtg
    32101 cgcgttgtgg cgaagtcttg acgggcaaat gtaagccgca tcaatgcccg ctgtttggta
    32161 acacctgtaa tcctcaaacc gcgtttggtg cgctgatggt ttcctccgaa ggagcgtgcg
    32221 ccgcgtggta tcagtatcgt cagcaggaga gtgaagcgtg aataatatcc aactcgccca
    32281 cggcagcggc ggccaggcga tgcagcaatt aatcaacagc ctgtttatgg aagcctttgc
    32341 caacccgtgg ctggcagagc aggaagatca ggcccgtctt gatttggcac agctggtagc
    32401 ggaaggcgac cgtctggcgt tctccaccga cagttacgtc attgacccgc tgttctttcc
    32461 tggcggtaat atcggcaagc tggcgatttg cggcaccgcc aatgacgttg cggtcagtgg
    32521 cgcaattccg cgctatctct cctgtggctt tatcctcgaa gaaggattgc cgatggagac
    32581 actgaaagcc gtagtgacca gcatggcaga aaccgcccgt gcggcaggca ttgccatcgt
    32641 tactggcgac accaaagtgg tacagcgcgg cgcggcagat aaactgttta tcaacaccgc
    32701 tggcatgggc gcaattccgg cgaatattca ctggggcgca cagacgctaa ccgcaggcga
    32761 tgtattgctg gttagcggta cactcggcga ccatggggcg actatcctta acctgcgtga
    32821 gcaactaggg ctggatggag aactggtcag cgactgcgcg gtgctggcgc cgcttattca
    32881 gacgctgcgt gacattcccg gcgtgaaagc gctgcgtgat gccacccgtg gtggtgtaaa
    32941 cgcggtggtt catgagttcg cggcagcctg cggttgtggt attgaacttt cagaagcggc
    33001 actgccggtt aaacctgctg tgcgcggcgt ttgcgaactg ctgggactgg acgcccttaa
    33061 ctttgccaac gaaggcaaac tggtgatcgc cgttgaacgc aacgcagcag agcaagtgct
    33121 ggcggcgtta cattcccatc cattgggaaa agatgcggcg ctgattggcg aagtagtaga
    33181 acgtaaaggt gttcgtcttg ccggtctgta tggcgtgaaa cgaaccctcg atctaccgca
    33241 cgccgaaccg cttccgcgta tatgctaata aaattctaaa tctcctatag ttagtcaatg
    33301 accttttgca ccgctttgcg gtgctttcct ggaagaacaa aatgtcatat acaccgatga
    33361 gtgatctcgg acagcaaggg ttgttcgaca tcactcggac actattgcag cagcccgatc
    33421 tggcctcgct gtgtgaggct ctttcgcaac tggtaaagcg ttctgcgctc gccgacaacg
    33481 cggcgattgt gttgtggcaa gcgcagactc aacgtgcgtc ttattacgca tcgcgtgaaa
    33541 aagacacccc cattaaatat gaagacgaaa ctgttctggc acacggtccg gtacgcagca
    33601 ttttgtcgcg ccctgatacg ctgcattgca gttacgaaga attttgtgaa acctggccgc
    33661 agctggccac aggtgggcta taccccaaat ttggtcacta ttgcctgatg ccactggcgg
    33721 cggaagggca tatttttggt ggctgtgaat ttattcgtta tgacgatcgc ccctggagcg
    33781 aaaaagagtt caatcgtctg caaacattta cgcagatcgt ttctgtcgtc accgaacaaa
    33841 tccagagccg cgtcgttaac aatgtcgact atgagttgtt atgccgggaa cgcgataact
    33901 tccgcatcct ggtcgccatc actaacgcgg tgctttcccg cctggatatg gacgaactgg
    33961 tcagcgaagt cgccaaagaa atccactact acttcgacat tgacgatatc agcatcgtct
    34021 tacgcagcca ccgtaagaac aaactcaaca tctactccac tcactatctt gataaacagc
    34081 atcccgctca cgaacagagc gaagtcgatg aagccggaac cctcaccgaa cgcgtcttca
    34141 aaagtaaaga gatgctgttg attaatctcc acgagcggga cgatttagcc ccctatgaac
    34201 gcatgttgtt caacacctgg ggcaatcaga ttcaaacctt gtgcctgtta ccgctgatgt
    34261 ctggcgacac catgctgggc gtactgaaac tggcgcaatg cgaagagaaa gtctttacca
    34321 ctaccaatct gaatttactg cgccagattg ccgaacgtgt ggcaatcgct gtcgataacg
    34381 ccctcgccta tcaggaaatt catcgtctga aagaacggct ggttgatgaa aacctcgccc
    34441 tgaccgagca gctcaacaat gttgatagtg aatttggcga gattattggc cgcagcgaag
    34501 ccatgtacag cgtacttaaa caagttgaaa tggtggcgca aagtgacagt accgttctga
    34561 tcctcggtga aactggtaca ggtaaagagc tgattgcccg cgcgatccat aatctcagtg
    34621 ggcgtaataa tcgccgcatg gtcaaaatga actgcgcggc gatgcctgcc ggattgctgg
    34681 aaagcgatct atttggtcat gagcgcgggg cttttaccgg tgccagcgct cagcgcattg
    34741 gtcgttttga actggcggat aaaagctcac tattcctcga tgaagtgggc gatatgccgc
    34801 tggagttaca gccgaagttg ctgcgtgtat tacaggaaca ggagtttgaa cgtctcggca
    34861 gcaacaaaat cattcagacg gacgtacgtt taattgccgc gactaaccgc gatctgaaaa
    34921 aaatggtcgc cgaccgagag ttccgtagcg atctctatta ccgcctgaac gtattcccga
    34981 tccacctgcc gccactacgc gagcgtccgg aagatattcc gctgctggcg aaagccttta
    35041 ccttcaaaat tgcccgtcgt ctggggcgca atatcgacag cattcctgcc gagacgctgc
    35101 gcatcctgag caacatggaa tggccgggca acgtgcgtga gctggaaaac gtcatcgagc
    35161 gcgcggtatt gctaacacgc ggcaacgtgc tgcagctgtc attgccagat attgctttac
    35221 cagaacctga aacgccgcct gccgcaacgg ttgtcgccca ggagggcgaa gatgaatatc
    35281 agttgattgt tcgcgtgctg aaagaaacaa acggcgtggt tgccgggcct aaaggcgctg
    35341 cgcaacgtct ggggctgaaa cgcacgactc tgctgtcacg aatgaagcga ctgggaattg
    35401 ataaatcggc attgatttaa cagatgtggt tatactcgat caacattcaa tgaaaggagg
    35461 cagtatggca gtttcagcaa gaaatcaatt aaccggaaca gttagcgcag tagcaatggg
    35521 cgcggtgaat gacgaagtag aactgacgct ggcaggtggt gcaaagctgg tggcaattgt
    35581 gactcacagc agccagcaag cgctgggtct ggcgaaaggg aaagaagcga ttgccctgat
    35641 taaagccccg tgggtaacgc tggcaacgga agactgtgga ctgaaattct ctgctcgcaa
    35701 ccagtttgct ggcagcgtgt cgacaatcac cgaaggcgca gtgaacgcca ctgtccatat
    35761 caaaacggat gcgggttttg aaattgtggc agtggtaaca aatgaaagtc aggatgaaat
    35821 gaaactcacc actggctcgc gcgttattgc attgatcaaa gcatcagcca tcctgattgc
    35881 caccaaagcg taagcgccgt atttgccgga gagtatggct ctccggcaga tttatttctt
    35941 tggacgatat ttttctggca actccggcac cggacgcttg tcatcgatga gatgacgcac
    36001 ggttaagatc ggatgacgcc acagcattct cggcccggcc caacgcataa tctgtttcat
    36061 ctcttcacgc tttgcaggct ggtaacagtg caccggacac tgcttacagg ctggtttctc
    36121 ttcgccgaac acacatttat ccagccgctt ttgcgcgtaa gcaaacaacg cctcgtaatg
    36181 ctccggctct gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat
    36241 cgtcagtttt tcacgagaga tacgcttgcc ggacatgctg cctccatact cttaaggtgc
    36301 atttatatta caacttaatt ttaaagggga ctatggctct gaaggaggag gtttcttcaa
    36361 ccgacgaatt tggcgatctt gtttcatcgt catcacgcaa aagctgcaaa gcagcatttt
    36421 tcgcggaacc gacatcaaga actcaccttt cgcgtcttcc cctgaaatga ttaactccgg
    36481 tatcatgtgc gccttatgtg attacaacga aaataaaaac catcacacta catttaacat
    36541 cagggagccg gacttaaccc catgagtaca atagaaaatt tcgacgccca tacgcccatg
    36601 atgcagcagt atctcaagct gaaagcccag catcccgaga tcctgctgtt ttaccggatg
    36661 ggcgactttt atgaactgtt ttatgacgac gcaaaacggg cgtcgcaact gctggatatt
    36721 tcactgacca aacgcggtgc ttcagcggga gagccaatcc cgatggcggg gattccctac
    36781 catgcggtgg aaaactacct cgccaaactg gtgaatcagg gcgaatccgt cgctatttgt
    36841 gaacagattg gcgatccggc gaccagcaaa gggccggtcg agcgcaaagt tgtccgtatc
    36901 gttacgccgg gcaccatcag cgatgaagcg ctgttgcagg aacgtcagga caacctgctg
    36961 gcggctatct ggcaggacag taaaggtttc ggctacgcaa cgctggatat cagttccggt
    37021 cgttttcgcc tgagcgaacc ggcggaccgc gaaacgatgg cggcagaact gcaacgtacg
    37081 aatccagcgg aattactgta tgcagaagat ttcgccgaga tgtcgctgat tgaaggtcgc
    37141 cgcggcctgc gtcgtcgccc gctgtgggag tttgaaatcg acaccgctcg ccagcaattg
    37201 aacctgcaat ttggcacccg agatctggtc ggttttggtg tggagaacgc accacgcgga
    37261 ctttgtgctg ccggttgtct gttgcagtat gcgaaagata cccaacgcac gaccctgccg
    37321 catatccgtt ctatcactat ggaacgtcag caggacagca tcattatgga tgccgcgacg
    37381 cgtcgtaacc tggaaattac tcagaacctg gccggcggtg cggaaaatac gctggcttca
    37441 gtgctcgact gcactgtaac gccgatgggt agtcgtatgt tgaaacgctg gctgcatatg
    37501 ccagtgcgcg atacccgcgt gttgcttgag cgtcagcaaa ctattggcgc attgcaggat
    37561 ttcaccgccg agctgcagcc ggtactgcgt caggtgggcg acctggaacg tattctggcg
    37621 cgtctggcgt tgcgtaccgc tcgcccacgc gatctggccc gtatgcgtca cgctttccag
    37681 caactgccgg agttgcgtgc gcagttagaa aatgtcgata gtgcaccggt acaagcgctg
    37741 cgtgagaaga tgggcgagtt tgccgagctg cgcgatctgc tggagcgagc aatcatcgac
    37801 acaccaccgg tgctggtacg cgacggtggt gttatcgcat cgggctataa cgaagagctg
    37861 gatgagtggc gcgcgctggc tgacggcgcg accgattatc tggagcgtct ggaagtccgc
    37921 gagcgtgaac gtaccggcct ggacacgctg aaagttggct ttaatgcggt gcacggctac
    37981 tacattcaaa tcagccgtgg gcaaagccat ttggcaccaa tcaactacat gcgtcgccag
    38041 acgctgaaaa acgccgagcg ctacatcatt ccagagctaa aagagtacga agataaagtt
    38101 ctcacctcaa aaggcaaagc actggctctg gaaaaacaac tttatgaaga gctgttcgac
    38161 ctgctgttgc cgcatctgga agcgttgcaa cagagcgcga gcgcgctggc ggaactcgac
    38221 gtgctggtga acctggcgga acgggcctat accctgaact atacctgccc gaccttcatt
    38281 gataaacctg gcattcgtat taccgaaggt cgccatccgg tagttgaaca agtactgaat
    38341 gagccgttta tcgctaaccc gctgaacctg tcgccgcagc gtcgcatgtt gatcattacc
    38401 ggtccgaaca tgggcggtaa aagtacctat atgcgccaga ccgcgttgat tgcgctgatg
    38461 gcgtatatcg gcagctacgt accggcgcaa aaagtcgaga ttggcccgat tgaccgtatc
    38521 tttacccgcg taggtgctgc ggatgatctg gcttccgggc gttcaacctt tatggtggag
    38581 atgaccgaaa ccgccaatat tttacataac gccaccgaat atagtctggt gttgatggat
    38641 gagatcgggc gtggaacgtc cacttacgat ggtctgtcgc tggcgtgggc gtgtgcggaa
    38701 aatctggcaa ataagatcaa agcgttgacg ctgtttgcta cccactattt cgagctgacc
    38761 cagttaccgg agaaaatgga aggcgtcgcc aacgtgcatc tcgatgcact ggagcacggc
    38821 gacaccattg cctttatgca tagcgtgcag gatggcgcgg cgagcaaaag ctacggcctg
    38881 gcggttgcag ctctggcggg tgtgccaaaa gaggttatta agcgcgcacg gcaaaaactg
    38941 cgtgagctgg aaagcatttc gccgaacgcc gctgctacgc aagtggatgg tacacaaatg
    39001 tctttgctgt ccgtaccgga agaaacttcg cctgcggtcg aggcactgga aaacctcgac
    39061 ccggattcac tgactccgcg tcaggcgctg gaatggattt atcgcttgaa gagtctggtg
    39121 taataataat tcccgatagc cttttgctat cgggaatatt aacgacaact gacgaatcaa
    39181 ataaaaatac cctgtataat aggaaaactt attttacagg gtaaaaccat gccatctaca
    39241 cgctatcaaa aaatcaatgc ccatcactat cgccatatat gggccgttgg tgatattcat
    39301 ggtgactatc aattattaca atcccgctta catcaactct ctttttgtcc tgaaaccgac
    39361 ttacttattt cggtaggcga taacatcgat cgtgggccgg agagtcttaa cgtgctgcgc
    39421 ctgttaaacc agccctggtt tatctccgtc aaaggcaacc acgaagcgat ggcgcttgag
    39481 gcattcgaaa ctggcgatgg aaatatgtgg cttgccagcg gtggtgactg gtttttcgat
    39541 ttaaatgatt cagagcaaca agaggcaata gatctgttgc tgaaattcca tctccttcca
    39601 catattattg aaatcactaa cgacaacata aaatatgtca tcgcacatgc agattatccg
    39661 gggaaggagt atcagtttgg caaagaaata gcggagagcg aattactctg gcctgttgat
    39721 cgtgtgcaga aatcacttaa tagcgaatta caaaaaataa acggcgccga cttctttata
    39781 tttggtcata tgatgtttga taacattcag acgttcgcta accagattta tatagatacc
    39841 ggatcgccga aaagcgggcg gctgtcattt tataaaataa agtagtctca tgcttcttct
    39901 gtgaagcatg agtaacccgg cgttattgca ggtcattatt catttttcgc taccagcaaa
    39961 gagagatcca gcttcgccag cgcgcggctg gcactctccg gcagaccgtc gtcagtaatg
    40021 atctgatcaa actcgcttaa tggtaacgcc agccatgtcg ccacctgtcc atatttcgtc
    40081 gcatcacaga ccaaaactcg ctggcggctg gcactggcaa tcgcccgttt caccgtgact
    40141 ttatcttctg ctggcgtaga gatcccccgc acactccatg acgatgcaga aataaaagcc
    40201 tgatcaatca tcaggctacg cagcatggtc gcagcggctt ccccaacaca ggaacggttt
    40261 tcccgacaca ctgcgccgcc agtgtgaata attgtgcaat tactgttgtc gagcaagtag
    40321 tccgcaataa cgaagtcgtt agtaaccact gtgagtggct ccatgtgaat cagatgctgt
    40381 gcaatcgcta acgtggtcgt tcccgcatcc agatagatac aacgtcccgg ctgaacaaga
    40441 cttgccgcca gcttgccaat agccgctttt tgcgtcattg ccagcgcagt ttttacctga
    40501 tgagaaggtt catgcgccac gcgtcccgga gactggacgc ccccggacac cagcacaacg
    40561 gctccctgct gctccagttt ttgtaaatcc cgacgaatgg tcatatgtga cacattcatt
    40621 ctgtccgtta gttcagcaat actaacaatg cctttttcag ctaccatctc aaggatgatt
    40681 tggcgacgct ctacgggtat caacttttgc tccttccttt gtcctgctga cattctacgc
    40741 tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag ttcacataaa ataacacaca
    40801 atgttaattt ttgtgaatca gctcaccata tcgttatctt tccgcgctta tattcacaat
    40861 atcaaacaaa atatcactta aattaacaag gagagcagat gaaaacggga tcggagtttc
    40921 atgtcggtat cgttggctta gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg
    40981 caggtctttc tacctggggc gcagacctga acagcaatgc ctgcgctacg ttgaaagagg
    41041 ctggtgcttg cggggtttct gataacgccg cgacgtttgc cgaaaaactg gacgctctgc
    41101 tggtgctggt ggtcaatgcg acccaggtta aacaggtgct gtttggtgaa aaaggcgttg
    41161 cacaacatct gaaacccggt acggcagtaa tggtttcttc cactatcgcc aatgctgatg
    41221 cgcaagaaat tgctaccgct ctggctggat tcggtctgga aatgctggat gcgccggttt
    41281 ctggtggtgc ggtaaaagcc gctaacggtg aaatgactgt catggcctcc ggtagcgata
    41341 ttgcctttga acgactggca cccgtgctgg aagccgttgc cggaaaagtt tatcgcatcg
    41401 gagcagaacc gggactgggt tcgaccgtaa aaattatcca ccagttgttg gcgggcgtac
    41461 atattgctgc cggagccgaa gcgatggcgc ttgcagcccg tgcggggatc ccgctggatg
    41521 tgatgtatga cgtcgtgacc aatgccgccg gaaattcatg gatgttcgaa aaccggatgc
    41581 gtcatgtggt ggatggcgat tacaccccgc attcagccgt cgatattttt gttaaggatc
    41641 ttggtctggt tgccgatacc gccaaagccc tgcacttccc gctgccattg gcctcaacag
    41701 cattgaatat gttcaccagc gccagtaacg cgggttacgg gaaagaagac gatagcgcag
    41761 ttatcaagat tttctctggc atcaccctac cgggagcgaa atcatgatca agattggcgt
    41821 tatcgctgat gattttaccg gcgcgacgga tatcgccagt tttctggtgg aaaacggtct
    41881 accaacggta caaattaacg gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact
    41941 ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa gccacacagc aatcgctggc
    42001 ggctctgagc tggttgcaac agcaaggttg caaacagatc tatttcaaat actgctctac
    42061 tttcgacagt acggcgaaag ggaatatcgg cccagttacc gatgcattaa tggatgctct
    42121 cgacacaccg tttacggtct tctctccggc cctgccagtc aacggacgta cggtttatca
    42181 ggggtatttg ttcgtaatga atcaactgct ggccgaatcc ggaatgcgcc atcacccggt
    42241 aaatcccatg accgacagct atcttccccg tctggttgaa gcgcaatcca cagggcgctg
    42301 cggcgtcgtt tcagcacatg ttttcgaaca aggtgtgaaa gccgttcgtc aggagctggc
    42361 tcgcttacag caagagggct atcgctacgc ggtgcttgat gcgctgaccg aacaccatct
    42421 ggaaattcag ggggaagcct tgcgcgatgc cccactggta acgggcggtt ctggtctggc
    42481 gattggcctg gcccggcagt gggcgcaaga aaacggtaac caggctcgcg aagcagggcg
    42541 tccgctcgct gggcgcggcg tagtgctctc cggttcatgc tctcaaatga ccaaccgcca
    42601 ggtggcacat taccgtcaaa ttgcaccagc ccgtgaggtt gatgtggcac gctgcctctc
    42661 aaccgaaact ctggccgctt atgcacacga gctggcagag tgggttctgg gccaggaaag
    42721 tgtacttgct ccactggttt ttgccaccgc cagcactgac gcattggcag caattcaaca
    42781 gcaatacggt gcacaaaaag ccagtcaggc agtagaaact ctgttttctc aactagcggc
    42841 gcggttagca gcggaaggcg tgacacgctt tattgtcgca ggcggtgaga cctccggcgt
    42901 agtcacacag agcctgggca taaaagggtt tcatattggc ccaaccattt cccccggcgt
    42961 gccgtgggta aacgcactgg ataagcctgt ctcactcgcc cttaaatctg gcaacttcgg
    43021 tgatgaagcc tttttttcac gagcccaaag agagttttta tcatgagcga tttcgcaaaa
    43081 gtagagcagt ctttgcgaga ggagatgacg cggattgcca gttcattctt tcagcgcgga
    43141 tatgcaaccg gttcggctgg caatctgtcg ctgcttttac ctgacgggaa tttactggcg
    43201 acaccgacag gttcatgcct gggcaatctc gatccgcagc ggctttccaa agtcaccgcg
    43261 gatggcgaat ggttaagtgg tgacaacccc tcgaaagagg tgctctttca tctggcgctg
    43321 tatcgcaaca atccgctctg taaagcggtg gtgcatttgc acagtacgtg gtcgacggcg
    43381 ctttcctgcc tggaagggct ggacagcaat aacgttattc gtccgttcac accatacgtg
    43441 gtgatgcgga tgggaaacgt cccgctggtg ccctattacc gaccgggcga taaacgcatc
    43501 gcacaggatc tggcggaact ggcagcagac aatcaggctt ttttactggc aaatcatggc
    43561 ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca atatggaaga gctggaggaa
    43621 acggcaaagc tgatttttat tctcggtgac cgcccgatcc gttatctgac cgcaggtgaa
    43681 attgcggaat taaggagtta atgcaatgcc tcgttttgca gctaatttat ccatgatgtt
    43741 caccgaagtg ccttttattg aacgcttcgc cgcagcgcga aaagccgggt tcgatgctgt
    43801 ggagtttctg tttccctatg actactcccc ccagcaaatc cagcagcaac tggtgcaaaa
    43861 ccatctgaca ctggcgctgt tcaataccgc tcccggagat attaatgcgg gagaatgggg
    43921 attatccgcc cttcccggac gtgagcacga agcacgcgca gacatcgacc tggcactgga
    43981 atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg gcaggcgtcg tgcccgctgg
    44041 cgaagatgct gaacggtacc gggcagtatt tattgataat ctccgctacg ctgccgaccg
    44101 ctttgcgcca cacggcaagc gaatattggt tgaagcactc agccccggcg ttaagcctca
    44161 ttatctcttc tccagccagt atcaggcact ggctattgtt gaagaggttg cgcgagataa
    44221 cgtgtttatt caactagata ctttccatgc gcaaaaagta gatggcaacc tgacacattt
    44281 aatccgcaac tacgccggga aatatgcgca cgtacaaatt gccggactac ctgatcgaca
    44341 tgaaccggac gatggagaaa tcaactatcc gtggctgttc cgcctgttcg atgaagtggg
    44401 atatcagggc tggatcggtt gtgaatacaa acctcgtggc ctcaccaaag aagggcttgg
    44461 ctggtttgac gcctggcgct aattcgctca atccctaatt acaacgtacc catacatccc
    44521 cccatctacg gatggggtag gttaactata actttcagac agggtttccc atgtccacaa
    44581 ttacattgtt atgcattgcg ttagctggcg taatcatgct gttgctactg gtcatcaagg
    44641 caaaggtaca accattcgtt gctctgctcc tcgtcagcct gttagtcgca cttgcggcgg
    44701 gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg gatgggcggt gtgcttggtt
    44761 ccgtcactat tattattggt cttggcgcta tgctggggcg tatgatcgaa cactctggtg
    44821 gtgcagagtc actggctaat tatttcagtc gcaagttagg tgacaaacga actatcgctg
    44881 cgctgactct ggcagcgttc ttcctcggca ttcccgtctt ctttgatgtc ggctttatta
    44941 ttcttgcgcc aatcatttac ggttttgcca aggttgccaa aatatcacca ctcaaatttg
    45001 gcctgcctgt cgctgggatc atgctcactg ttcacgtggc ggtaccgccg catccaggcc
    45061 ctgtcgccgc agcgggatta ctccacgcag acatcggctg gctaaccatc atcggtattg
    45121 cgatttctat tcccgtaggg attgttggct actttgcagc gaaaataatc aataagcgcc
    45181 aatatgcgat gtcggtcgag gtactggaac agatgcaact ggctccggcc agtgaggaag
    45241 gcgcgacaaa attaagcgat aaaataaatc caccgggcgt cgcgttagtc acctcgctaa
    45301 ttgttattcc tatcgcgatt atcatggcgg gaacggtttc tgcaacactg atgccgcctt
    45361 cgcatcccct gcttggtacg ctacagttga tcggctcacc aatggtagct ctaatgattg
    45421 cgctggtgct ggcattctgg ttattggctt tacgtcgcgg ctggagctta caacacacca
    45481 gcgacattat gggctctgcc cttcccactg cggcggtagt gattttggtt actggtgctg
    45541 gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg taaagccctt gccaacatgt
    45601 tacaaatgat tgacctgcct ctgttaccag ccgcatttat tatttcactg gcgctgcgtg
    45661 catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg cgggttactc tcagaagcgg
    45721 tgatgggatt gaatccaatt cagtgcgtat tggtgacgct ggcagcctgc tttggtgggc
    45781 ttggcgcgtc acatattaat gactcagggt tctggattgt gaccaaatat ctggggttgt
    45841 cggtagcaga cggtctgaaa acctggacag tgttaacgac cattctcggt tttaccggat
    45901 tcttaattac ctggtgcgta tggctcgtca tctaataatc cagataagat atgatgtatc
    45961 gtttaagtgc ctgataaggg tgtaatgtat gccaatatta acctcggtaa tatctcgcta
    46021 ccgaggttaa ttttcacttt gcaatataat tacagtctct tgcaagttga ttttatctta
    46081 gtaataagaa ccgaccaacc ttattgccat ttcgatatta gcaggcatag atgcgtcgta
    46141 gccatacata tggccatatt tcggacgtaa tacttcagtt acttttttaa taattgcttc
    46201 gcgctgggct cgcgcaaaat cagcatgatt atttgccgtt ttattcataa atttaccaaa
    46261 actttcttct tttctgactt taattgtcgt atcccgaaga tcattaagaa actggcgttg
    46321 aaatttaaaa tcatcgtgtg aaccaatatt accatgcccg ccaataaaat atttccagtc
    46381 aagcatttca acctgacgca aattactgtc atggtaaacc aacgtatccg atacggcgaa
    46441 gcccatcatc ggcagttggt caggattcag gagatccggg ctgtgagcta ctttttcctg
    46501 ttttagcaac catacagaat ggtcgtcggt atgtcctgct ttaacaaatc ggtgtaattc
    46561 aatggtctgc ttttcaaatt taaactggtc atctttttta cttaaaacct gagtggcgac
    46621 gggtaagcga ctgttcataa attgcatttt ttcagcagtg gctttactgg caataatacg
    46681 cagattcacg ccttctttct tcgcctcatc gttccaaaat ggactatcgc caatgtgatc
    46741 cacatggtaa tgagaataaa ccagcgctgt tacgggtaat ggagtcacat cacgaattgc
    46801 ctgtaataaa tatttaccgc gaccctccgg agcatcaaac agcaaaaccc ctttgtctcc
    46861 gacataaaac gtagtggaat aaaaatagcg ttgaacataa taagttctct cgccaatttt
    46921 ttgtaaaata taaggctgag acatatttct ttcaaaaagg ttattgagtt tatcgcttgg
    46981 cgatattatt ttttccgctg aagg
//
LOCUS       DACADF010000017        46697 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.017, whole genome shotgun sequence.
ACCESSION   DACADF010000017 DACADF010000000
VERSION     DACADF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.017"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            3..329
                     /locus_tag="ISB30_000961"
     CDS             3..329
                     /locus_tag="ISB30_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988622.1"
                     /translation="MHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKQNIFKP
                     DIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDP
                     SSTAHE"
     gene            complement(313..951)
                     /gene="ytfB"
                     /locus_tag="ISB30_000962"
     CDS             complement(313..951)
                     /gene="ytfB"
                     /locus_tag="ISB30_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HAP1988623.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIILVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     ESQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1170..1790
                     /locus_tag="ISB30_000963"
     CDS             1170..1790
                     /locus_tag="ISB30_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAP1988624.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2099..3511
                     /gene="cycA"
                     /locus_tag="ISB30_000964"
                     /gene_synonym="dagA"
     CDS             2099..3511
                     /gene="cycA"
                     /locus_tag="ISB30_000964"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAP1988625.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3556..4218)
                     /gene="ytfE"
                     /locus_tag="ISB30_000965"
     CDS             complement(3556..4218)
                     /gene="ytfE"
                     /locus_tag="ISB30_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAP1988626.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4326..5291)
                     /locus_tag="ISB30_000966"
     CDS             complement(4326..5291)
                     /locus_tag="ISB30_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP1988627.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5399..6259)
                     /gene="qorB"
                     /locus_tag="ISB30_000967"
     CDS             complement(5399..6259)
                     /gene="qorB"
                     /locus_tag="ISB30_000967"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAP1988628.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETDKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6348..6728
                     /locus_tag="ISB30_000968"
     CDS             6348..6728
                     /locus_tag="ISB30_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP1988629.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6857..8800)
                     /gene="cpdB"
                     /locus_tag="ISB30_000969"
     CDS             complement(6857..8800)
                     /gene="cpdB"
                     /locus_tag="ISB30_000969"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAP1988630.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     LVVVLAHSGLSADPYKVMAENSVYYLSEIPGVDAIMFGHAHAVFPSKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8990..9730
                     /gene="cysQ"
                     /locus_tag="ISB30_000970"
     CDS             8990..9730
                     /gene="cysQ"
                     /locus_tag="ISB30_000970"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAP1988631.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(9720..10277)
                     /locus_tag="ISB30_000971"
     CDS             complement(9720..10277)
                     /locus_tag="ISB30_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAP1988632.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            10602..10808
                     /locus_tag="ISB30_000972"
     CDS             10602..10808
                     /locus_tag="ISB30_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAP1988633.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(10870..12213)
                     /locus_tag="ISB30_000973"
     CDS             complement(10870..12213)
                     /locus_tag="ISB30_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP1988634.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(12536..13174)
                     /gene="msrA"
                     /locus_tag="ISB30_000974"
     CDS             complement(12536..13174)
                     /gene="msrA"
                     /locus_tag="ISB30_000974"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP1988635.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            13380..15113
                     /gene="tamA"
                     /locus_tag="ISB30_000975"
     CDS             13380..15113
                     /gene="tamA"
                     /locus_tag="ISB30_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAP1988636.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            15110..18889
                     /gene="tamB"
                     /locus_tag="ISB30_000976"
     CDS             15110..18889
                     /gene="tamB"
                     /locus_tag="ISB30_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAP1988637.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKLKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            18892..19233
                     /locus_tag="ISB30_000977"
     CDS             18892..19233
                     /locus_tag="ISB30_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAP1988638.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            19445..19696
                     /locus_tag="ISB30_000978"
     CDS             19445..19696
                     /locus_tag="ISB30_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ChpS family
                     antitoxin"
                     /protein_id="HAP1988639.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            19690..20040
                     /gene="chpB"
                     /locus_tag="ISB30_000979"
     CDS             19690..20040
                     /gene="chpB"
                     /locus_tag="ISB30_000979"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HAP1988640.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(20120..20650)
                     /gene="ppa"
                     /locus_tag="ISB30_000980"
     CDS             complement(20120..20650)
                     /gene="ppa"
                     /locus_tag="ISB30_000980"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAP1988641.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            20960..21916
                     /gene="ytfQ"
                     /locus_tag="ISB30_000981"
     CDS             20960..21916
                     /gene="ytfQ"
                     /locus_tag="ISB30_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HAP1988642.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            22056..23558
                     /locus_tag="ISB30_000982"
     CDS             22056..23558
                     /locus_tag="ISB30_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP1988643.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            23572..24594
                     /locus_tag="ISB30_000983"
     CDS             23572..24594
                     /locus_tag="ISB30_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1988644.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            24581..25576
                     /gene="yjfF"
                     /locus_tag="ISB30_000984"
     CDS             24581..25576
                     /gene="yjfF"
                     /locus_tag="ISB30_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAP1988645.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(25609..26607)
                     /gene="fbp"
                     /locus_tag="ISB30_000985"
     CDS             complement(25609..26607)
                     /gene="fbp"
                     /locus_tag="ISB30_000985"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAP1988646.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            26783..28156
                     /gene="mpl"
                     /locus_tag="ISB30_000986"
     CDS             26783..28156
                     /gene="mpl"
                     /locus_tag="ISB30_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAP1988647.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(28307..28858)
                     /locus_tag="ISB30_000987"
     CDS             complement(28307..28858)
                     /locus_tag="ISB30_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAP1988648.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            28952..30304
                     /gene="pmbA"
                     /locus_tag="ISB30_000988"
     CDS             28952..30304
                     /gene="pmbA"
                     /locus_tag="ISB30_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAP1988649.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            30487..30873
                     /gene="cybC"
                     /locus_tag="ISB30_000989"
     CDS             30487..30873
                     /gene="cybC"
                     /locus_tag="ISB30_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAP1988650.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(30918..31382)
                     /gene="nrdG"
                     /locus_tag="ISB30_000990"
     CDS             complement(30918..31382)
                     /gene="nrdG"
                     /locus_tag="ISB30_000990"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAP1988651.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(31541..33679)
                     /gene="nrdD"
                     /locus_tag="ISB30_000991"
     CDS             complement(31541..33679)
                     /gene="nrdD"
                     /locus_tag="ISB30_000991"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP1988652.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWSIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(34073..35728)
                     /gene="treC"
                     /locus_tag="ISB30_000992"
     CDS             complement(34073..35728)
                     /gene="treC"
                     /locus_tag="ISB30_000992"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP1988653.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(35778..37199)
                     /gene="treB"
                     /locus_tag="ISB30_000993"
     CDS             complement(35778..37199)
                     /gene="treB"
                     /locus_tag="ISB30_000993"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAP1988654.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGNFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(37318..38265)
                     /gene="treR"
                     /locus_tag="ISB30_000994"
     CDS             complement(37318..38265)
                     /gene="treR"
                     /locus_tag="ISB30_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAP1988655.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            38450..38503
                     /gene="mgtL"
                     /locus_tag="ISB30_000995"
     CDS             38450..38503
                     /gene="mgtL"
                     /locus_tag="ISB30_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAP1988656.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            38644..41340
                     /gene="mgtA"
                     /locus_tag="ISB30_000996"
     CDS             38644..41340
                     /gene="mgtA"
                     /locus_tag="ISB30_000996"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP1988657.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(41546..41932)
                     /gene="ridA"
                     /locus_tag="ISB30_000997"
     CDS             complement(41546..41932)
                     /gene="ridA"
                     /locus_tag="ISB30_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAP1988658.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(42005..42466)
                     /gene="pyrI"
                     /locus_tag="ISB30_000998"
     CDS             complement(42005..42466)
                     /gene="pyrI"
                     /locus_tag="ISB30_000998"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAP1988659.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(42479..43414)
                     /gene="pyrB"
                     /locus_tag="ISB30_000999"
     CDS             complement(42479..43414)
                     /gene="pyrB"
                     /locus_tag="ISB30_000999"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAP1988660.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(43418..43552)
                     /gene="pyrL"
                     /locus_tag="ISB30_001000"
     CDS             complement(43418..43552)
                     /gene="pyrL"
                     /locus_tag="ISB30_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAP1988661.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(43833..44228)
                     /locus_tag="ISB30_001001"
     CDS             complement(43833..44228)
                     /locus_tag="ISB30_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAP1988662.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(44359..45072)
                     /gene="bdcA"
                     /locus_tag="ISB30_001002"
     CDS             complement(44359..45072)
                     /gene="bdcA"
                     /locus_tag="ISB30_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1988663.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAHETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            45143..45736
                     /locus_tag="ISB30_001003"
     CDS             45143..45736
                     /locus_tag="ISB30_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1988664.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGEAIKTILKE"
     gene            45881..46333
                     /locus_tag="ISB30_001004"
     CDS             45881..46333
                     /locus_tag="ISB30_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAP1988665.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYKARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPETDWLVDKDIAF
                     LPEVVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            46456..>46697
                     /locus_tag="ISB30_001005"
     CDS             46456..>46697
                     /locus_tag="ISB30_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988666.1"
                     /translation="MAKISDLNCYQHMASADNVKPKNEVLNTWCVGMNDFARNAEGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKCSNEAGRN"
BASE COUNT        11068 a        11718 c        12516 g        11395 t
ORIGIN      
        1 ttgtgcatag tgaaaatcaa agtaagggtg tgcattatgc aaaatcacag cgtcttttag
       61 aaattaacca tgcgcacttg cagttaatgg aatccttatt ggatgaaggg aagaaacaaa
      121 atatttttaa gccagatatc gacccattgc aggtgaatat taatattgct gcgcttggcg
      181 gatattattt gatcaaccag catacgcttg gcctggttta tcatatcagt atggtttccc
      241 cccaggcact ggaagccaga cgtaaggtca tcaaagagac aatccttagc tggcttttgg
      301 ttgacccttc atctaccgcg cacgaataaa actgccatcc ggctggcggg tgaacaggac
      361 ctgttgatta ttccccgtat caatggttaa gcccgtcacc acgccgctgg cgttctggcg
      421 gattttcacc atttgcccgt tttgcaaatt actcagcggc ttacccgcgc cttctacctg
      481 cgccattgca tagacatcgg tagcgggcag tccgtggtcg cggaacagct gcgccatcgt
      541 tttaccgggt tcgacacggt aagagcgcca ttgattatca attccgctat ctggctggaa
      601 tggttgtgtt tgggtagttt gcggctgttc ttctggctgg ctttcctgta taggttctgg
      661 agcgaccgga gccacctgat caggatcatt ttgtggtgtg actaattgcg cccgcagctg
      721 ttcttctgtc ggcggttgtg attgcgactg aatatctaac tgcgcttcac gcgtcaccac
      781 cggcgcgttg ggcgtatcat cagatggaag cagaaatccg accaccaata taatggcggc
      841 aataatgatc ccccgacgat gcatcggcgg cagcgggtcc atgaagcgaa aattgtccgg
      901 cgcgtgccag actttctcca gggttggttt tagttcaaag cgcccgggca tggtttcctc
      961 ctgctccgcg tcttgttcct caatcatagc ctatgaataa gctaacgcta tgatgtccgt
     1021 ggtaaacccg cctttattat attcatacgg gatattgcta ttgtttcttt ttccccggga
     1081 tttgtcatca ttcctgcgac aaagttttac ccaaagaagt gtggctgata tgctgcccgc
     1141 tacttttata ccctaagaaa ggaaatacga tgaccacccc aacttttgac accatcgaag
     1201 cgcaagcaag ctacggcatt ggtttgcagg tagggcaaca actgagtgaa tctggcctgg
     1261 aagggctgct gccagaagca ctggttgcag gtattgccga tgcgctggaa ggcaaacatc
     1321 cggctgttcc ggttgatgtg gtgcatcgcg cgctgcgtga aatccacgag cgcgccgatg
     1381 ccgttcgtcg tcagcgtttc caggcgatgg ctgctgaagg tgtgaaatac ctggaagaaa
     1441 acgccaaaaa agaaggtgtg aatagcaccg aatctggcct gcaattccgc gtgatcaacc
     1501 agggtgaagg cgcaattccg gcacgtaccg accgcgttcg tgttcattac accggtaaac
     1561 tgatcgacgg caccgtgttt gacagctccg ttgctcgtgg tgaacccgct gaatttccgg
     1621 ttaatggcgt gatccctggc tggattgaag cactgactct gatgccggta ggttctaaat
     1681 gggaactgac tatcccgcag gaactggcat atggcgagcg cggcgcaggc gcatccatcc
     1741 ctccgttcag caccctggtg tttgaagtcg aactgctgga aatcctctaa gcagcgcatt
     1801 ctgttcccct cgaaccagag gggagcaggc attcagcaat aaacccttca gtttgccaaa
     1861 cggcgctatt ttgtgttgca aagaccccgt aagcgtgtat ttttgtgagc tgtttcgcgt
     1921 tatcaccgtg atatgacact cactttaaac ataaaattaa cattagatct aaatcttagt
     1981 attcatcccg cgtattgtta cctaatatcg atgagtcccg atacagattc gtcgtatcat
     2041 agactgacta aaggccgtag agcctgaaca acacagacag gtacaggaag aaaaaaacat
     2101 ggtagatcag gtaaaagtcg ttgccgatga tcaggctccg gctgaacagt cgctacggcg
     2161 caatctcaca aaccgacata ttcagcttat tgccattggc ggtgccattg gtacggggtt
     2221 gtttatgggg tctggcaaaa cgattagcct tgccgggccg tcgatcattt tcgtttatat
     2281 gatcatcggt tttatgctct ttttcgtgat gcgggcaatg ggggaattgc tgctttcgaa
     2341 tctggaatac aaatctttta gtgacttcgc ttccgattta ctcgggccgt gggcaggata
     2401 tttcaccggc tggacttact ggttctgctg ggttgtaacc ggtatggcag acgtggtggc
     2461 gatcactgct tatgctcagt tctggttccc cgatctctcc gactgggtcg cctcgctggc
     2521 ggtgatagtg ctgctgctga cgctcaatct cgccaccgtg aaaatgttcg gtgagatgga
     2581 gttctggttt gcgatgatca aaatcgtcgc cattgtgtcg ctgattgtcg tcggcctggt
     2641 tatggtggcg atgcactttc agtcaccgac tggtgtggaa gcgtcattcg cgcatttgtg
     2701 gaatgatggc ggctggttcc cgaaaggttt aagtggcttc tttgccggat tccagatagc
     2761 ggttttcgct ttcgtaggga ttgagctggt aggtacaacc gctgcggaaa ccaaagatcc
     2821 ggagaaatca ctgccgcgtg caattaactc tattccgatc cgtatcatta tgttctacgt
     2881 cttcgcgctg attgtaatta tgtccgtgac gccgtggagt tcggtagtcc cggagaaaag
     2941 cccgtttgtt gaactgttcg tgttggtagg gctgcctgct gccgcaagcg tgatcaactt
     3001 tgtggtgctg acctctgcgg cgtcttccgc taacagcggc gtcttctcta ccagccgtat
     3061 gctgtttggt ctggcgcagg aaggtgtagc accgaaagcg ttcgctaaac tttctaagcg
     3121 cgcagtaccc gcgaaagggt tgaccttctc gtgtatctgt ctgctcggcg gcgtggtgat
     3181 gttgtatgtg aatcctagcg tgattggcgc gttcacgatg attacaaccg tttccgcgat
     3241 tctgtttatg ttcgtctgga caattatcct ttgctcgtac ctggtttacc gcaaacagcg
     3301 tcctcatctg catgagaagt cgatctacaa gatgccgctc ggcaagctga tgtgctgggt
     3361 atgtatggcg ttctttgtgt tcgtggtcgt gttgctgaca ctggaagatg acactcgcca
     3421 ggcgctgttg gtcaccccgc tgtggtttat cgcactgggg ctgggctggt tgtttattgg
     3481 taagaagcgg gctgctgaac tgcggaaata accgcattat catgctggat ggcgcaatgc
     3541 catccagctt ttagattact cacccgccag cgcgcgtggg aacagtacat tgttttccag
     3601 gctgatgtga tccatcaggt catcaatcag ttcattaata ccgttataca tcgctttcca
     3661 ggtggtgcag gcttccggcg gcggtgtgac gttattggtg gtgtgtttaa tcacttccag
     3721 cagttcgccc gcttcatcgt gctcgctttc catcacgctg attggtccca ttgcctggct
     3781 gcccatgcct tgtttgatca tcgggaagag gatctgctct tctttcatca tgtggctgga
     3841 aagctcttca tgcagcatag tcaggtattt tgtcagccct tttggcacgc tcggtttgtc
     3901 ggcgtgaacg cgctcgactt tagtcgcttg caggatcagc tccggcagct gctcgcggtg
     3961 acgatcgtgg tagcgcacga tgatatggtc aatgatttct gccagcgggg cgctacgcca
     4021 gtctttctca atcggttgtt cagcaagctt tgccagttca gcttcaatga cctcaacatc
     4081 cagttcttta cgtgccgccg cgcgcgccag cgtctgctta ccgccacagc agtaatccat
     4141 atcatattta cgaaacagag ccgaagcgcg agggatagag agcgccagtt cacctaaagg
     4201 ttggtcgcga taagccatag ctgatacctc attcttaata atataagatg tattttaaat
     4261 gcatctttaa ggcaaaagct ataaccctta cgtagcagag gcttatttaa ctcactgcca
     4321 gcgtgtcaac tttctgagac agattcagtt aaaggttttt ctggttttgc tctgaccgca
     4381 atgaccacgc caatcactaa cagcgcgata ccgctcagcg tcattagcgg cggcatttgc
     4441 tggcggagta aaaaggtgta cagcaaacct gccagcgttt cgaaaacaat cagcggcccg
     4501 agaatcactg tcggtaatcg ctggctggcg acgttccagc agagtgcgcc aacccatgag
     4561 caaagcacgg ctatcgcaac catcagacta ataaacacca gcggacgggg gccaaaaggt
     4621 aaggagaagt ccggcgtttg tatattcagc cagtaacagg cgacgagata gccgatgaga
     4681 gaaaccggca gcgtgaccag cgcctgcgcc gtcgcccaca tcatcggatg tttgtcggga
     4741 ttttcccgca gccagcgggc gttgcgcagg gcataccatg cccagcagac cacggaaact
     4801 aacgctagca cgatgccaga ggtataacgt gcccagtcaa aatcggggag tccgtggttt
     4861 aactcagcaa tattcacact cgccaggccg atgccaatac aaatcagtgc cggggcgagt
     4921 tttccccacg cgagtttgcc gtcgcgctgg ctataaagca gattggcaaa gacaggaatg
     4981 accaccggca gggtgccgat aatcatcgtg gaaacaggcg cgccagtacg ttgaatggca
     5041 ctggcaaggc agaagtaata aatgaggttg cccatcatag tgagcatcaa ggcggtaagc
     5101 cagtcccgac gcgccaactg acgcagacgc acgcgtccca gccaggcaat gggcagcgca
     5161 attaacccta acgccagata acgccccatc gactgcaaca tcgccgggta ttccggtacg
     5221 atcaacggcc cgacaaaaat aagcccccac atcaaccctg ctaacagggc gtacagcacg
     5281 ccgctaatca ttactggcat ccattggtct gtcagaagaa agttcaggaa gcagacatga
     5341 ttgctcccgg catcccgtca ttattagaga ggatgcggga ggatgccact ttaattaact
     5401 agttattaac attaaaaaga tggcttacgc tttcggctaa cgttgtcgtt gggcggccaa
     5461 tcaatttgct aagcgttttg ctgttatcaa acagaccacc tttcgatgcg ccaacgtcag
     5521 aatccgccag catatccgcc agtccgtcgg gcagtccgac gcttttcagt gccgcggcga
     5581 aatcggcttc gctcagattt tgataggtaa cggattttcc gctctgtttg gtcagctctg
     5641 ccgctaactg tgtcaacgtc caggcactat cgcccgccag ttcgtaaacc ttgccttcgt
     5701 gaccggcttc gctaatcacg cgtgccgcag ctgccgcata atctgcccgc gttgctgagg
     5761 cgattttgcc atcgcccgcc gcaccgataa atacgccgtg ttccagtgct gccggggcgc
     5821 tggcgaggta gttttcggtg taccagccgt tgcgcagcag ggtgtaaacg atgccagaat
     5881 cagccagcat tttatccgtc tcgatgtgct catcggcgag gccgagcggg gaggtatctg
     5941 catgtagcag gctggtataa gcgataaatt tcacgccagc cgcctttgcg gcattaataa
     6001 cattacgatg ctgcggggca cgttgaccca cttcgctgga agagatcagc agtagttttt
     6061 ccactccctg aagtgcagat gtcagtgcgg cttcatcgcc gtagtcagcc tgacgcacgg
     6121 taatgccttg tgctgccagg gcctgggctt ttgccggatt acgaacgata gccactattt
     6181 ggctggcagg aaccgttttc atcaaggatt caataacata gtgaccaagt tggccagtgg
     6241 caccagtaat agcgatcatg ggaagtctcc atcgttttgt cttgtgttta aatacgctaa
     6301 cacctaaact tacttttagt aagtacgtac aaaaaggtaa gtatgaaatg agtcaggtta
     6361 gcctgtcgca acaactgaaa gagggtaatc tctttgcgga acagtgcccg tcgcgcgagg
     6421 tgttgaaaca cgtcaccagc cgttgggggg tattgattct ggtggcgcta cgcgaaggta
     6481 ctcatcgctt tagcgacctg cggcgcaaaa tcggtggggt gagtgaaaag atgcttgcgc
     6541 agtcgttaca ggcgttagaa caggatggtt ttcttaaccg tatcgcgtat ccggtggtgc
     6601 cgccgcatgt ggaatacagc ctcacgccgc tgggcgagca ggtgagcgaa aaggttgctg
     6661 cactggcgga ctggattgag ttgaatttgc ccgaggtgtt ggcggtgcgg gatgaacgtg
     6721 cggcataact gccggatgcg ctgagcttat ctggtctacg gtgcatggct tgtagggcgg
     6781 ataagatgcg ccagcatcgc atccgccaat aaatgccgga tgcggttgat gatttatagg
     6841 ccgaaaaaga agtgttttac ttactcaaat ccacctgata aatcgcaaac ccgatatcat
     6901 cggtcgcgac tttattcatc ggatattgcc ctttctcttt aataaacgct gcggctttat
     6961 ctgacgggga agtttcgaaa cggatatcca gtttcttatc gccagcaatc ggtgctaaac
     7021 gccagttgtt atctgccgcc gggtgaattt cccccgcacg tttcgactca tcagcaatcc
     7081 acgctgccag caccgagcgg ttctcatccg gtgaagcaaa agcgatatgg ctgtcgcccg
     7141 taccggcaaa tttgccgccg taagcgcgat agttattggt ggcaacgagg aacatggcgt
     7201 tcggatcaat cggcttgcca ttaaaggtca ggttcttaat cctttccgca ttggcattaa
     7261 tcatctggca ctcgccgtca taacgggcag gctgggtaac atcaatctga taattcacac
     7321 catcaatcac atcaaagtta taggtgcgga aaccatccca gttgatgagt gattgcggtt
     7381 tcgtgctgtt aggatcaatc tggttaaact gtccggcgga gcactccagc cactctttca
     7441 cctctttacc gctggctttc actacaatca gcgtattggg gtagagataa agatcggcgg
     7501 cattacggaa ggtcaactgg cctttttcca cctccacata gcttgccggg tcatttttgc
     7561 gaccaccaac tttaaacggt gcggcagctg aaagcaccgg cagttttgcc agatccggat
     7621 cgccctgaat gtaatgttcg acatacgctt tttgcgcgtt gttcactact tgcacggtcg
     7681 gatcgtcctg caccagcgcc agatagctat acatattgtc ggcagattta ccgattggct
     7741 tgctgacgaa ctggcgtgtg gcatcgtgat cggctttgag tgtttctacc agcttgctgt
     7801 cttccgccgc gagggatttt ttattggcga tgtcgtaaat cggccgagct tccgctttcg
     7861 cctgcgtcac ctgccattta ccgctgtcat tactgagttg taagtcgacc acaccaagat
     7921 gatcgcccca catgcctggc attaccgccg gaacaccatt cagcgtgcct ttcgcgatat
     7981 cagctccttc gatatcagca aaatctttac ttgggaaaac ggcgtgagca tggccaaaca
     8041 taatggcgtc aacacccgga atttcactga gataataaac tgagttttcc gccatcactt
     8101 tatacggatc ggcagacagc ccggaatgcg ccagaacgac aacgagatcg gcacctttct
     8161 cgcgcatttc aggcacgtat ttgcgcacgg tttcggtaat atcattcacc gtcactttcc
     8221 cggataaatt agctttatcc cagcccatga tttgcggcgg tacgacgcca atatagccaa
     8281 tcttcagcgt ctgttttttt ccgtctttat cgaccacttc ggtgtcttta attaaatacg
     8341 gtgtaaacat tggctgtttg gttctggcgt caatgacgtt ggcatttaca taagggaatt
     8401 tcgctcccgc cagcgcattt ttcaggtaat ccagaccgta gttaaactca tggttgccaa
     8461 gcgtgccgac ggtatagtcc agcgtattta atgccttata cactgggtga atatcacctg
     8521 cttttaatcc tttcgccgac atgtaatctg ccagcggact cccctgaatc aaatcgccgt
     8581 tatcgaccag tacgctgttt ttcacttcat tgcgggcatc gttaatcagg cttgccgtac
     8641 gtaccagtcc gaatttttcc gtggcggtgt ctttgtaata atcgaaatcc atcatgttgc
     8701 tatgcagatc agtggtttcc atgatacgca gatcgaccgt cgctgcattc acactggcgg
     8761 caatcagcgt ggccaggagc gttgcgctaa acttaatcat cagggacatc cttttatcat
     8821 cgggaatacg aaagaaaagg gagaataaac gtcttactta tagaacagtg aagaatgcca
     8881 caattttacg ctttgaaaat gatgacacta tcacagttgg cgcattcatt aacgataggg
     8941 tataagtaaa acaataagtt aacaccgctc acagagacga ggtggagaaa tgttagatca
     9001 agtatgccag cttgcacgga atgcaggcga tgccattatg caggtctacg acgggacgaa
     9061 accgatggac gtcgtcagca aagcggacaa ttctccggta acggcagcgg atattgccgc
     9121 acacaccgtt atcatggacg gtttacgtac gctgacaccg gatattccgg tcctttctga
     9181 agaagatcct cccggttggg aagtccgtca gcactggcag cgttactggc tggtagaccc
     9241 gctggatggt actaaagagt ttattaaacg caatggcgaa ttcaccgtta acattgcgct
     9301 cattgaccat ggcaaaccga ttttaggtgt ggtgtatgcg ccggtaatga acgtaatgta
     9361 cagcgcggca gaaggcaagg cctggaaaga agagtgcggt gtgcgcaagc agattcaggt
     9421 ccgcgatgcg cgcccgccgc tggtggttat cagccgttcc catgcagatg cggagctgaa
     9481 agagtatctg caacagcttg gcgaacatca gaccacgtcc atcggctctt cgctgaaatt
     9541 ctgcctggtg gcggaaggac aggcgcagct gtacccgcgc ttcggaccaa cgaatatttg
     9601 ggacaccgcc gctggacatg ctgtggccgc agctgccgga gcgcacgttc acgactggca
     9661 gggtaaaccg ctggattaca ctccgcgcga gtcgttcctg aatcctgggt tcagagtgtc
     9721 tatttattaa ttaatttatg cagcaggtcc atcacttgct gcacctcttc ctgagtaagc
     9781 gccccgtctt tggcccattg cacgcgaccg tctttatcca gcaccactac agcggaactt
     9841 ttttcatcca gctgccaggc accgcgtgcg acgccattgc tatcaacaat aaactgcgac
     9901 cagggataaa gctttttatt actctccaga ctgctgcgca caaacatgcc ggaacccgga
     9961 attgcgtcgt cggtgttaac aatggtggtg gtctggtaac gatcgtgcgg taacttcgct
    10021 gatttaatcg cttcaatcag cgtcgcgttt ttctcttttg cagaggtgcg accagcaata
    10081 tgttgcagta ctcgcacttt tcccactaac tgcgcgctgt tccaggtttt gtagctaaac
    10141 tgatctttat caagcaccaa ctcgccacga tcggtgatgc caatcggcgg cactcgctga
    10201 ccggtttcga actgatgtgc ggaagccatc atcggcaaca gcaggcaggt gagtgccaga
    10261 atcttgcgta gggtcatggt gtttccttct tatgatatgc aggtgatccg accacttggg
    10321 tctgagtttt aatcatatgt gctatttatc gaatttcccg caagtgtgat gccagtttgc
    10381 ggtcaagcgc acaaatcata tgaaaaatga atgcttatac tgaagaccgc gcttcggtaa
    10441 aaagataatt ctgaataatt gtaaccttta ggtaaaaaaa gttatacgcg gtggaaacat
    10501 tgcccggata gtctatagtc actaggcatt aaaatttgcg cctcataata gttgggccga
    10561 ttgtggcacc gcacaggcgt aatactcagc aggagataac aatgaaaatt ttccaacggt
    10621 acaacccact tcaggtggcg aagtacgtaa agatcctgtt ccgtggacgg ttatacatca
    10681 aggacgttgg cgcttttgaa tttgataagg gtaagattct tatcccaaaa gtgaaggata
    10741 agctgcattt gtcagtgatg tccgaagtta accgtcaggt tatgcgtctg caaacagaga
    10801 tggcttaacc aaagtgctat gcagtaataa gacggctcct gattcaggag ccgttgatgt
    10861 ttctgggggt tacgcgacgc tttcttcttt atctttagcg tcaggcagtt ttggcgaaag
    10921 ggcggtggcc ttgctgtcga tccgggtcac cagcagctgg tcgatgcggt agttatcgat
    10981 atccaccact tcaaatttgt agccggcgaa tttcaccgaa tcggtgcgtt tcgggatctt
    11041 acgcagcata aacatcataa agcctccgat ggtttcgtag ttgcccgact gcgggaactc
    11101 gtcaatatcc agcacgcgca tgacgtcgtc aattggggtg ccgccgtcaa tcagccatga
    11161 gttctcatca cgggcgacaa tctgctcttc cagcccctga ccgaccagat cgcccatcag
    11221 cgtggtcatc acgtcgttga gggtgatgat ccccaccacc agcgcgtact cgttcatgat
    11281 caccgcgaag tcttcacctg cggttttaaa actttccaac gcctctgaaa gggttaacgt
    11341 atccggcaca atcagcgtgt tgcgaatttg tacgccgctg ttcagtgcca ggctttggtt
    11401 agccagcacg cggttcagca ggtctttaga atcgacataa ccgatgatgt ggtcaatatc
    11461 ttcattacag acgaggaact tagagtgcgg atgttccgcc accttattct tcaggctttg
    11521 ctcatcttcg tggagatcaa accaaatcac gttttcacgc ggtgtcattg aagacggaac
    11581 ggtacgggat tccagctcaa agacgttttc aatcagctcg tgttcctgtt tacgtaacac
    11641 gcccgccagc gcaccggctt ccactaccgc gtagatgtca tcagaagtga tgtcatcttt
    11701 acgtaccatt ggcagtttga aaatacggaa gatcatgttc gccaggccgt tgaagaacca
    11761 caccagcggg gtgcaaacgt acaggcagaa gcgcatcggg ttgatgatac gcaaagccac
    11821 cgcttctggc gcaatcatac cgatgcgttt cggggttaaa tccgcaaaca ggataaacat
    11881 gccagtcact aacgagaaag agagaataaa gctcagttgc tcagagagtt ctgccgacat
    11941 atagcgggag aacaggctgt gaaaagctgg agaaaatgcc gcatcaccga cgataccgcc
    12001 gagaatcgca actgcgttca gaccgatttg gaccaccgta aagaacatgc cgggattttc
    12061 ctgcatattc agaacgcgtt gggcatttat attgccttca tcagccagca gtttaagttt
    12121 gattttgcgt gaggcggcaa gtgagatctc agacatcgag aagaacgcac ttacagcgat
    12181 caagcagagt atgactaaaa tactgtttaa catatcttat ccgacctttc aggccagatc
    12241 ctcggaaggg gaagttgatt aatttgtgtg aaatacacat tgaaagccga ttcgaagaaa
    12301 gtgaaccggc attttcagtg gctagtatag cgtaaggtac tgtaaagtcg ccagagggtt
    12361 aaaattcaga ctgctgataa atgcaatact gcctgatgcg ctatgccgat caggcctacg
    12421 cagtccctgc aatatattga atttggacga ttttgtaggc ctggtaaggc attcacgccg
    12481 catctggcat aaataaagcg cactttatca acaatctaca tttgtacccg taacgctatg
    12541 cttccggtgg cagacagacg ccaattccgc caattccaca gtaaccatac gggtttttat
    12601 gcagatattg ctggtggtca tcttcggcat aataaaacgg tgtggcgtta gcgatttccg
    12661 tggtgatgtg acgatcgtca tcggcggcaa gcatcgccgc ctgaaaacgt tccagactgg
    12721 cgcgagctgc ggcatcctgt tctggggtca gcggataaat cgctgaacga tactgcgtgc
    12781 cgtggtcatt gccctgacgc attccctggg cgggatcgtg attctcccaa aatacctgta
    12841 gcaactgctc atagctgatg acggaaggat cgtaaacaat gcgtaccgct tcggcatgac
    12901 ccgtatcacc ggagcacact tcccgataag tcggatttgg cgtatagcct ccggtatagc
    12961 ctgcggcggt gctgtaaacg ccgggtaact gccagaacag acgctccact ccccagaaac
    13021 aacccatcgc aaaaatggca atctccattc cgtcaggtac attggtcatt gagtgaccgt
    13081 tgaccgcatg cagcgtggct acgggcatcg gggtgttacg tccaggcagg gcatcggcgg
    13141 gggaaaccag atgcttttta tcaaataaac tcatggtgtc gctctcccga aaatcggtca
    13201 ttggggttaa ggttgtaaca agaggcgtat ttgcacacaa taaccaccgt gaataggtct
    13261 aaagtaaaac ataagaaata tttggggttt agtctgcttt ttaatccata ttactggatt
    13321 tttgttaagc cgtttaacgg cgttccaggg gcaggaaaaa aggatattca ggagaaaatg
    13381 tgcgctatat ccgacagtta tgctgtgtaa gcttactctg cttaagcgga tctgccgtcg
    13441 ccgcgaacgt ccgtctacag gtcgaggggt tatcgggaca gctggaaaag aacgttcgtg
    13501 cgcagctttc tacgattgaa agtgatgaag tgacgccaga ccgtcgcttt cgcgcacgcg
    13561 tcgatgatgc catccgcgaa ggtctgaaag cgctgggtta ttaccagccg accattgaat
    13621 ttgatctccg tccaccgcca aagaaagggc ggcaggtatt gatcgccaaa gtcacgccag
    13681 gtgtgccggt gttaattggc ggcaccgatg tggtattgcg cggcggcgcg cggaccgata
    13741 aagactattt gaaattgctc gatactcgcc cggctattgg cacggtgctg aaccagggcg
    13801 attatgaaaa tttcaaaaag tccttaacca gcattgcgtt gcgtaaaggt tatttcgata
    13861 gcgaatttac caaagcgcag ctgggcattg cgctcggcct gcataaagcc ttctgggata
    13921 ttgattataa cagtggcgaa cgttaccgct ttgggcatgt gacctttgaa ggatcacaaa
    13981 tccgcgatga atacctgcaa aatctggtgc cgtttaaaga gggcgatgag tacgaatcga
    14041 aagatctggc ggaactgaac cgccgacttt ctgctaccgg ctggtttaac tcggtggtgg
    14101 tggctccaca atttgataaa gcgcgcgaaa cgaaagtatt acccttgacg ggcgtggttt
    14161 cgccgcgaac tgaaaacacc atcgaaaccg gggtcggtta ctctacggac gtgggaccgc
    14221 gcgtgaaagc gacgtggaaa aagccgtgga tgaactctta tggtcacagt ctgaccacca
    14281 gtaccagtat ttccgcgccg gaacagaccc tcgacttcag ctataaaatg ccgctgctga
    14341 agaatccact ggaacaatat tatttggtgc agggcggttt taagcgcact gacctgaacg
    14401 ataccgaatc tgactccact acgctggtgg cttctcgcta ctgggatctc tccagcggct
    14461 ggcagcgtgc cattaacctg cgctggagcc tcgaccactt tacccagggt gaaattacca
    14521 acaccacgat gctgttttat cctggggtga tgattagccg cacgcgttct cgtggtggcc
    14581 tgatgccaac ctggggcgac tcgcaacgct actctatcga ctactccaac acggcctggg
    14641 gttcagatgt cgatttctcc gttttccagg cgcagaacgt ctggatccgc acactgtacg
    14701 atcgccatcg tttcgttaca cgcggcacgc tgggctggat tgaaaccggt gatttcgaca
    14761 aagtaccgcc ggatctgcgt ttcttcgccg ggggcgatcg cagtattcgc ggctacaaat
    14821 acaaatctat cgccccgaaa tacgccaacg gtgacctgaa aggggcctcg aagttgataa
    14881 ccggatcgct ggaataccag tacaacgtga ccggaaaatg gtggggcgcg gtgtttgtcg
    14941 atagtggcga agcggtaagc gatattcgcc gcagcgactt taaaaccggt accggggtcg
    15001 gcgtgcgctg ggaatcgccg gtcgggccaa tcaaactcga ttttgccgta ccggtcgcgg
    15061 ataaagacga acacgggtta cagttttaca tcggtctggg gccagaatta tgagtttatg
    15121 gaaaaaaatc agcctcggcg tggttatcgt tatcttactg ttgctgggat cggtggcgtt
    15181 tctggtgggc accaccagcg gcctgcatct tgtatttaaa gcggcggatc gctgggtgcc
    15241 aggactggat attggcaagg tcaccggcgg ctggcgcgat ctcaccttgt ctgacgttcg
    15301 ttatgagcag ccaggcgtgg cggtaaaagc gggtaatctg catctggcgg tcggtcttga
    15361 gtgcctgtgg aacagtagcg tttgtattaa tgacctggcg ctgaaagaca ttcaggtcaa
    15421 catcgacagt aaaaaaatgc ctccttctga acaggttgaa gaagaggaag atagcggtcc
    15481 gctggatctc tccacgccgt atcccatcac cctgacacgg gtggcgctgg acaacgtcaa
    15541 catcaagatt gatgacacca cggtgtcggt gatggacttc acctccggcc tgaactggca
    15601 ggagaaaacc ctgaccctga aaccgacgtc gctgaaaggc ctgctgattg ctctgccgaa
    15661 agtggcggaa gtggcgcagg aagaagtggt cgaaccgaaa attgaaaatc cgcagccgga
    15721 ggaaaagccg ctcggcgaaa cgctgaaaga tctcttttct cgcccggtat tgccggaaat
    15781 gaccgacgtg catttgccgc ttaacctgaa cattgaagag tttaaaggcg agcagttacg
    15841 cgtgacgggc gacacggaca tcaccgtgcg caccatgctg ctgaaagtga gcagcattga
    15901 cggcaatact aaactggacg ccctggatat cgattccagt caagggatcg tcaacgccag
    15961 cggcacggcg cagctgtcag acaactggcc ggtggatatc accctcaaca gcacactgaa
    16021 cgtggagccg ttgaaaggag aaaaactgaa gctgaaagtg ggcggtgcgc tgcgcgaaca
    16081 gctggagatt ggcgttaacc tttccggtcc ggtggatatg gatttacgcg cccagacgcg
    16141 actggcggaa gccggattgc cgctcaacgt ggaagtgaac agcaaacagc tttactggcc
    16201 gttcactggt gagaagcagt atcaggcgga tgatctgaaa ctgaaactta ccggcaaaat
    16261 gaccgattac acgctctcta tgcgtacggc agtgaaggga caggagatcc cgccagccac
    16321 cattaccctt gatgccaaag gtaatgaaca gcaggtcaat ctcgacaaac tcaccgtcgc
    16381 ggcgctggaa gggaaaactg aactcaaggc gttgctcgac tggcagcagg ccattagttg
    16441 gcgcggtgag ctaacgctta atggcattaa caccgccaaa gagttcccgg agtggccgtc
    16501 gaaactcaat ggcttgatta aaacccgcgg tagcctgtac ggcggcacct ggcagatgga
    16561 ggtgccagaa ctgaagctga ccggtaacgt caaacagaac aaagtgaacg ttgacggcac
    16621 gctgaaaggc aacagttata tgcagtggat gatcccaggg ctgcatctgg aactcgggcc
    16681 aaacagtgcc gaagtgaaag gcgagctggg ggtaaaagat ctcaatcttg atgccaccat
    16741 caacgcgccg gggctggata acgcgctgcc ggggcttggc ggtacagcga aagggctggt
    16801 gaaagtacgc ggcacggtgg aagcgccaca actactggca gatatcaccg cgcgcggcct
    16861 gcgctggcag gaactttccg tggcgcaggt tcgcgtggaa ggcgacatta aatccaccga
    16921 tcagatcgcc gggaaactcg acgtacgcgt tgagcaaatt tcgcagccgg atgtaaatat
    16981 caacctcgtc accctgaatg ccaaaggcag cgaaaagcag cacgagctac agttgcggat
    17041 tcagggcgag cctgtctccg ggcagcttaa tctggcagga agttttgatc gcaaagaaga
    17101 acgctggaag ggaactctta gcaatacccg cttccagacg ccggtcggcc cgtggtcgct
    17161 gacccgcgat attgcgctgg attaccgcaa taaggagcaa aaaatcagca tcgggccaca
    17221 ctgctggctt aacccgaatg cggaactgtg cgtgccgcaa actatcgatg cgggggccga
    17281 agggcgtgcg gtggtgaatc tcaaccgctt cgacctcgcc atgctgaaac cgtttatgcc
    17341 agaaaccact caggccagcg gtatcttcac gggtaaagcg gatgttgcct gggacaccac
    17401 gaaagagggg ctgccgcagg gcagtatcac cctttcgggg cgtaacgtgc aggtaacgca
    17461 aaccgtcaac gatgcggcgc tgcccgtggc gtttcagaca ctgaatctga cggcggaatt
    17521 gcgtaacaac cgtgccgaat tgggctggac catccgcctg accaataacg gccagtttga
    17581 tggacaggtg caggtgaccg atccgcaagg ccgccgtaat cttggtggca acgtcaatat
    17641 ccgtaacttc aaccttgcga tgataaaccc catctttaac cgtggggaaa aagcagcggg
    17701 gatggtgagt gccaacttgc gtctgggtgg tgatgtgcaa agcccgcagt tgtttggtca
    17761 gcttcaggtt acgggtgtgg atatcgacgg caactttatg ccgtttgata tgcagccgag
    17821 ccagcttgcg gtcaacttta acggtatgcg ctcgacgctt gccggtacag tacggaccca
    17881 gcagggtgaa atctacctga acggtgatgc cgactggagc caaattgaaa actggcgggc
    17941 acgagtaacg gcgaaaggca gtaaagtgcg gatcaccgtg ccgccgatgg tacgaatgga
    18001 tgtatcgcca gatgttgtat tcgaggctac accaaacctg tttaccctcg atggtcgcgt
    18061 ggatgtcccg tgggcgcgca tcgtggtgca cgatctgccg gaaagcgcag taggcgtctc
    18121 cagcgatgtg gtgatgctta acgataacct gcaaccggaa gagccgaaaa cggcgtcgat
    18181 tccgattaac agtaacctga ttgtccacgt tggcaacaat gtgcgcattg acgcctttgg
    18241 cctgaaagcg cggctgacgg gcgatcttaa cgtcgttcag gacaaacaag ggctgggcct
    18301 gaacgggcag atcaacatcc ctgaagggcg cttccatgcc tatggtcagg atctgattgt
    18361 gcgtaagggt gagttactgt tctctggtcc gccggatcaa ccgtatctta atattgaagc
    18421 tattcgtaac ccggatgcta cagaagacga cgtaatcgcc ggagttcgcg tcactggtct
    18481 ggcggacgaa ccgaaagcgg agatcttctc tgacccggcg atgtcgcaac aagctgcatt
    18541 gtcttatttg ctacgtggac aagggctgga gagcgatcag agcgacagtg cggcaatgac
    18601 ctcgatgctg attggtctgg gggttgcgca aagtggccag attgtgggta aaatcggcga
    18661 gacgtttggc gtaagcaatt tagcgctcga cacccaggga gtaggcgact cctcccaggt
    18721 agtagtcagc ggctatgtat tgccaggtct gcaagtgaaa tacggcgtgg gtatatttga
    18781 ctctatagca acactcacgt tacgttatcg cctgatgcct aagctatatc tggaagccgt
    18841 gtctggtgta gaccaggcac tggatttgct ctatcagttc gagttttagc aatgcgaata
    18901 tttgtctacg gcagtttacg ccacaaacaa ggcaacagtc actggatgac caatgcccag
    18961 ttactgggcg atttcagtat cgataactac cagttgtata gcctgggcca ctatccaggc
    19021 gcagttccgg ggaacggaac agtacacggt gaagtttatc gtattgacaa cgccacgctg
    19081 gccgaacttg atgccttgcg caccaggggc ggtgaatacg cgcgccagtt gattcagacg
    19141 ccgtacggga gtgcatggat gtacgtttat caacgacccg tcgatggatt aaagctaatt
    19201 gaaagcggcg actggttaga cagggataag taaccatatg catacgccac cttcgggtgg
    19261 cgttgttttt tgcgagacga ctcgcattct gttttgtaat tccctcaccc tttgcttttc
    19321 tctccgagcc gctttccata tctgttaacg cataaaaaac tctgctggcg ttcacaaatg
    19381 cgcaggggta aaacgtttcc tgtagcaccg tgagttatac tttgtataac ttaaggaggt
    19441 gcagatgcgt attaccataa aaagatgggg gaacagtgca ggtatggtca ttcccaatat
    19501 cgtaatgaaa gaacttaact tacagccggg gcagagcgtg gaggcgcaag tgagcaacaa
    19561 tcaactgatt ctgacaccca tctccaggcg ctactcgctt gatgaactgc tggcacagtg
    19621 tgacatgaac gccgcggaac ttagcgagca ggatgtctgg ggtaaatcca cccctgcggg
    19681 tgacgaaata tggtaaagaa aagtgaattt gaacggggag acattgtgct ggttggcttt
    19741 gatccagcaa gcggccatga acagcaaggt gctggtcgac ctgcgcttgt gctctccgtt
    19801 caagccttta atcaactggg aatgacgctg gtggccccca ttacgcaggg cggaaatttt
    19861 gcccgttatg ccggatttag cgttccttta cattgcgaag aaggcgatgt gcacggcgtg
    19921 gtgctggtga atcaggtgcg gatgatggat ctacgcgccc ggctggcaaa gcgtattggt
    19981 ctggctgcgg atgaggtggt ggaagaggcg ttattacgct tgcaggcggt ggtggaataa
    20041 ggtgtgttta tttatcgcgg gcataaaaaa acccttacta accgaagctc ggcgttcagg
    20101 gttattgcgc tagaagaact tatttattct ttgcgcgctc gaaggaagca acgatttcag
    20161 ctttagcggc ttctgcgttt tcccaacctt caactttcac ccacttgcct ttttcgaggt
    20221 ctttgtagtg ctcgaagaag tgagcgatct gcgctttcag cagctctggc agatcgttaa
    20281 cgtctttaat gtgatcgtat tctttgctca gcttgctgtg cggaaccgca accagtttcg
    20341 catcttcacc ggcttcgtcg gtcattttca gaacgccaac cggacggcaa cggatcacag
    20401 aacccggctg cagcgggtac ggagtcggga ccagtacgtc aaccgggtca ccgtccagag
    20461 acagggtgtg gttgatgtaa ccgtagttgc acggatagaa catcgcggtg gacatgaagc
    20521 ggtcaacgaa cagtgcgccg ctctctttgt cgatttcgta tttgatcgga tctgcgttag
    20581 ccgggatctc aataaccacg tagatgtctt ccggcagatc tttacccgca gggacgttga
    20641 gtaagctcat gtctgtttcc tttaaaaata tgtggcaaac aagtgccgag tattatagcc
    20701 aactcgcgcc gaatgtcttt gcttgttttc gtcttcattt tcctttttcg ccagttttaa
    20761 gacggtatac agagcaggaa aatccataac cgtagccgca tttttcatag tgagatgaaa
    20821 gcgattacaa acttgtgatt aacgttttat ttactttttt gaagtgtgat gtaacgcaat
    20881 ccgttacata acgaattgtc tatagttttt tcgcgaacat cttttcacca ataataatta
    20941 ccccgacgag gacaacccta tgtggaaacg cttacttgta gtctctgcag tctcggcagc
    21001 catgtcgtct atggcgttgg ccgctccatt aaccgtagga ttttcgcagg tcggatcgga
    21061 atccggctgg cgcgctgcag aaaccaatgt ggcgaaaagt gaggccgaaa aacgcggaat
    21121 tacgttgaaa attgccgatg gtcagcaaaa gcaggaaaac cagattaaag cggtacgttc
    21181 cttcgttgca caaggggtgg atgcgatctt tatcgctcca gtggttgcga cgggttggga
    21241 accggtatta aaagaggcga aagatgccga aatcccggtc ttcttgcttg accgttccat
    21301 cgatgtgaaa gacaaatctc tctatatgac caccgtcact gccgacaaca tcctcgaagg
    21361 caagttgatt ggtgactggc tggtaaaaga agtgaatggc aaaccatgca acgtggtgga
    21421 actgcagggc accgttgggg ccagcgtcgc cattgaccgt aagaaaggct ttgccgaagc
    21481 cattaagaat gcgccaaata tcaaaatcat ccgctcgcag tcaggtgact tcacccgcag
    21541 taaaggcaaa gaagttatgg agagctttat caaagcggaa aacaacggca aaaacatctg
    21601 catggtttac gcccataacg atgacatggt gattggcgca attcaggcaa ttaaagaagc
    21661 gggcctgaaa ccgggcaaag atatcctcac gggttccatt gacggtgtac ccgatatcta
    21721 caaagcgatg attgatggcg aagcgaacgc cagtgttgaa ctgacgccga atatggcagg
    21781 tcccgccttc gacgcgctgg agaaatacaa aaaagacggc accatgcctg aaaagctgac
    21841 gctgaccaaa tccacccttt atctgcctga taccgcaaaa gaagagttag agaagaagaa
    21901 aaatatgggg tattgagggt tgctatgcct gatgccgatt cgtaggccgg ataaggcgct
    21961 cgcgccgcat ccggcgatgg tgcactgaag cctgatgcga cgcttaccgc gtcttatcat
    22021 gcctactggg agcacgcttt acaccggggg aaaccatgac gaccgaccaa caccaggaga
    22081 tcctccgcac cgaaggatta agtaaatttt tccccggcgt caaagcgtta gacaacgttg
    22141 atttcagcct gcgccgtggc gaaatcatgg cgctgcttgg tgaaaacggg gcgggaaaat
    22201 caacgctaat caaagcatta actggtgtat accacgccga tcgcggcacc atctggctgg
    22261 aaggccaggc tatctcaccg aaaaataccg cccatgcaca acaactcggt atcggcaccg
    22321 tctatcagga agtcaacctg ctacccaata tgtcggttgc tgataatcta tttataggcc
    22381 gcgaacccaa acgtttcggc cttctacgcc gtaaagagat ggaaaagcgc gccaccgaac
    22441 tgatggcatc ttacggtttc tccctcgacg tgcgcgaacc gcttaaccgt ttttcggtgg
    22501 cgatgcagca aatcgtcgcc atttgccggg ctatcgatct ctccgccaaa gtgctgatcc
    22561 tcgatgaacc caccgccagc ctcgacaccc aggaagtgga gctattattt ggcctgatgc
    22621 gtcagttgcg cgatcgcggc gtcagcctga tctttgtcac tcactttctc gatcaggtct
    22681 atcaggtcag cgatcggatc accgtcttac gcaacggcag tttcgtaggc tgtcgggaaa
    22741 cgcgcgagct accgcagatc gaactggtaa aaatgatgct ggggcgcgag ctggataccc
    22801 acgcgctaca gcgtgccggg cgaacattgt tgagcgacaa acccgttgcc gcgttcaaaa
    22861 attacggcaa aaaaggaacg atagcaccgt ttgatctcga agtgcgcccc ggcgagatcg
    22921 tcggtctggc tggcttgctg ggatccggac gtaccgaaac cgccgaagtg atcttcggta
    22981 tcaaacctgc tgacagcggc acggcgttga tcaaaggcaa accgcaaacc ctgcgatcgc
    23041 cacatcaggc ttcagtactg ggcatcggat tctgcccgga agacaggaaa accgatggca
    23101 tcatcgctgc cgcctcggtg cgggaaaata tcatcctcgc tctccaggcc cagcgcggct
    23161 ggctacgacc catttcccgc aaagagcagc aagagattgc cgaacgcttt atccgccagc
    23221 ttggcattcg cacaccttca actgaacaac cgattgaatt tctctctggc ggcaatcagc
    23281 aaaaagtgtt gctttcacgt tggctactga cccgaccgca atttctgatc ctcgacgagc
    23341 caacccgcgg cattgatgtt ggtgcccacg ccgagatcat ccgcctgatt gaaacgctgt
    23401 gtgctgacgg tctggcgctg ttggtgatct cctccgaact ggaagagctg gtgggctatg
    23461 ccgatcgggt gattatcatg cgcgatcgca aacaggtggc ggagatcccg ctggcagagc
    23521 tttccgttcc ggcgatcatg aacgccattg cggcgtaagg agaacagtgt gatgcctcaa
    23581 tctctcccgg acactacgcc gccgaaaagg cgctttcgct ggccaacggg aatgccgcag
    23641 ctggcggcgc tattgctggt gctgctggtc gatagcctgg tagccccgca tttctggcag
    23701 gtggtgcttc aggatgggcg tttgttcggt agccctatcg acattcttaa ccgtgcagct
    23761 cccgttgcgt tactggcgat tggtatgacg ctggtgatcg ccaccggtgg gattgatctc
    23821 tccgtagggg cggtgatggc tatcgccgga gccacaacgg ctgcgatgac ggtcgcggga
    23881 ttcagcctgc cgattgtttt gttaagcgcc ctgggcaccg gcatcctggc gggattgtgg
    23941 aacggcatac tggtagcgat cctcaaaatt cagccgtttg tcgccaccct gatcctgatg
    24001 gtcgccgggc gcggcgtggc gcaactgatc accgccgggc agatcgtcac gtttaactcg
    24061 ccggatctct catggtttgg cagcggatcg ctgttgttcc tgccaacgcc ggtcattatc
    24121 gcggtgctga cgcttctcct gttctggctg ttgacccgca aaacggcgct ggggatgttt
    24181 atcgaagccg ttggtatcaa cattcgggcg gcaaaaaatg ccggggtaaa cacgcggatc
    24241 atcgtcatgc tcacctacgt gttgagcggg ctgtgtgcgg cgattgcggg cattatcgtg
    24301 gcggcggata ttcgcggtgc cgatgccaat aacgccgggt tatggctgga gctggacgcc
    24361 attcttgcgg tggtgattgg cggcggatcg ctgatgggcg ggcgctttaa cctgctgctc
    24421 tcagtcgtgg gggcgctgat tattcagggg atgaacaccg gaattttact ttctggcttt
    24481 ccgccggaga tgaaccaggt cgtaaaagcg gtggtggtgc tttgtgtgct gattgttcag
    24541 tcgcaacgct ttatcagtct gattaaagga gtacgtagcc atgataaaac gtaacttgcc
    24601 gctgatgatc accatcggcg tctttgtgct gggctatctc tactgcctga cccagtttcc
    24661 cggttttgct tccacaagag tgatctgcaa tatcctgacc gataacgcct ttcttgggat
    24721 aatcgccgtt ggcatgacct ttgtgatcct ctctggcggt atcgatctct ccgtcggttc
    24781 ggtgatcgcc tttactggcg tgtttctggc aaaagtgatt ggcgatttcg gcctctcgcc
    24841 gctgctggcg tttccgctag tgctggtgat gggctgtgcc tttggcgcat ttatggggct
    24901 tttgatcgac gccctgaaga tcccggcatt tatcattacc ctggcaggaa tgttcttttt
    24961 gcgcggcgtc agctatctcg tttcagaaga gtcgatcccg ataaaccatc ccatttatga
    25021 cacgctctca agccttgcgt ggaaaatccc tggcggcggt cgcttaagtg cgatgggact
    25081 gctgatgctg gcggtggtgg ttatcggcat attcctcgcg catcgtaccc gttttggtaa
    25141 tcaggtatac gccattggcg gcaacgcaac gtcggcaaac ctgatgggga tttctacccg
    25201 cagcaccact attcgcattt atatgctctc caccggactg gcaacgctgg cggggattgt
    25261 cttctcgatt tatacccagg ccggatatgc gctggcgggc gtaggtgtgg aactggatgc
    25321 tatcgcctca gtggtaattg gcggtacgct tttgagcggt ggcgttggaa cggtattagg
    25381 gacgcttttt ggcgtggcga ttcaggggct aattcagact tatatcaact ttgacggtac
    25441 gctgagttcc tggtggacga aaatcgccat cggcattttg ctgttcattt ttattgcctt
    25501 acagcgtgga ttaacggtgc tgtgggagaa tcgtcagagt tcgccagtga caagagtcaa
    25561 tattgcgcag cgataaaaac gcctctccgt gtggagaggc gcaggagatt acgcgtccgg
    25621 gaattcacgg ataaagcgtt cgacatcttc aaccatatgg tcgttgccga caaagaacga
    25681 acggcgctgg tgcagagttt ccgggatgat atccagaata cgctctttgc catcgctcgc
    25741 tttaccgccc gcttgttccg ccaggaatgc catcgggttg cactcataca gcaaacgcag
    25801 tttgccgtcc gggtggctgg cggtgcttgg gtagagataa ataccgcctt tcagcaggtt
    25861 acggtggaaa tccgcgacca gtgaaccgat ataacgtgag gtatacgggc ggttggtgga
    25921 tttatcttct tcctggcaga atttaatgta cttcttcacc ccgttcggaa acttaatgta
    25981 gtttccttcg ttgatggagt aggttttgcc tttctccggg aagcgcatcc gttcctggca
    26041 caggcagaaa acgcccagcg aaggatcgta agtaaaggcg tgaacaccgc atccggtggt
    26101 gtaaaccagc atggtagagg agccgtatac cacgtaacct gccgcaacct gtttgttacc
    26161 aggctggagg aaatcttctt ccgttaccgg cgtgccaaca ggcgtaacgc ggcggtagat
    26221 ggagaaaatg gtaccgacag agacgttaac atcgatgttg gacgagccat ccagggggtc
    26281 catcagcacc acgtattttg cgtgttcaca gccttcaaag acgacaatct catcttcttc
    26341 ttcagaggca atgcccgcaa cgatatcgcg tgctttcagt gcggctttca gtttttcatt
    26401 agcgaacaag tcgagtttct gctgaacctc gccctgcacg ttctcagcac cgctggcacc
    26461 caggatatca accagtcctg ctttgttgat atcgcgatgg ataatcttgg cgcccagttt
    26521 tattgccgac agcaaagcag tgagctcacc ggtagcatga gaaaactcgt gctgcttttc
    26581 gacaataaat tcacctaacg ttttcataaa actttccctg caatgtttat ggagtaaagc
    26641 gaccgcaaca atcttaacaa ataatctcaa tgttgcgctc aggtgaatcg cgccagcaaa
    26701 ttgcggatta tcctgaaatg cgtttctcac ttgcccgaca tatgcgtaaa atgagcggca
    26761 gattaaaaaa ggatagtgac gtatgcgcat tcatatttta ggaatttgtg gcacatttat
    26821 gggcggtctg gcgatgctgg cgcgccagtt aggccatgaa gtaacgggtt cggacgccaa
    26881 tgtgtatccg ccgatgagca ccttacttga gaagcaaggc attgagctga ttcagggtta
    26941 cgatgccagc cagctcgatc cgcagccgga tctggtgatt attggcaacg ccatgacccg
    27001 tggaaatccg tgtgtggaag cggtactgga aaaaaacatc ccttatatgt caggtccaca
    27061 gtggctgcac gattttgtgc tgcgcgaccg ctgggtgctg gccgttgccg gtacacacgg
    27121 caaaactacc accgcgggaa tggcgacctg gattctggaa cagtgcggtt acaaaccggg
    27181 atttgtgatc ggcggtgtgc cggggaactt tgaggtttcg gcgcgtctgg gcgaaagcga
    27241 cttctttgtt atcgaagcgg atgagtatga ctgcgccttc ttcgacaaac gctctaaatt
    27301 tgtccattac tgcccgcgta cgctgatcct caacaacctt gagttcgatc acgccgatat
    27361 ctttgacgac ctgaaagcga tccagaaaca gttccaccat ctggtgcgta tcgttccggg
    27421 gcagggccgt attatctggc cggaaaatga catcaacctg aaacagacca tggcgatggg
    27481 ctgctggagc gagcaggagc tggtgggtga gcagggtcac tggcaggcga aaaagctgac
    27541 cactgacgct tctgaatggg aagtcttgct ggatggcgaa aaagtgggcg aagtgaaatg
    27601 gtcgctggtg ggcgaacata atatgcacaa tggcctgatg gcgattgcgg cggctcgcca
    27661 tgttggtgta gcgcctgcag atgccgctaa cgcgctgggt tcgtttatta acgctcgtcg
    27721 ccgtctggag ttgcgtggtg aagcgaatgg cgtgacggta tatgacgatt ttgcccatca
    27781 cccgacggca attctggcaa cgcttgcggc gctgcgtggc aaagttggtg gtacggcgcg
    27841 cattattgct gtgctggagc cgcgctcgaa taccatgaaa atggggatct gcaaagacga
    27901 tctggcacct tcattaggtc gtgccgatga agtcttcctg ctgcaaccgg cgcatattcc
    27961 gtggcaggtg gcagaagtgg cagaagcctg cgttcagcct gcacactgga gtggcgatgt
    28021 ggatacgctg gcagatatgg tggtgaaaac cgctcagcct ggcgaccata ttctggtcat
    28081 gagcaacggc ggttttggtg ggatccatca gaaactgctg gatggtctgg cgaagaaggc
    28141 ggaagctgcg cagtaattcg gcctcagcct gagatagcat tgccggataa ggcgtttacg
    28201 ccgcatccgg catttgagca tagtgcctga tgcgacgctt gatgcgtctt atccggccta
    28261 caggtgcacc gcatccggca aaccatactc acatcaacaa cgaaaattac ccttcattct
    28321 ccgccagttc gcgcagatac tggaaaatct ggcgtgcgga tttcggcggc ttattccctt
    28381 ctttctcttt cttcgcgtta cggatcagag tacgcagctg ctgacgatcg gcatcgggcc
    28441 acagattcaa tacttcagcg atggcgtcat caccttgatc gatcagacga tcgcgcaggt
    28501 tttccagttt atggaacagc accacctgct ggttgtgacg gtttttcagc ttatccagcg
    28561 cctgacgaat aggctctacg tcgcgctggc gcagcatctt accgatgagc tgcaactggc
    28621 ggcggcgacc ttccatctta atacgctggg cgagctcaat agccgcgcgc agatccgcat
    28681 ctaacgggat cttatccagc gcgtttttcc ccagatcaac aatttccgcg ccaaggcgtt
    28741 ttagctcctc ggcatcacgt ttaatttcac ttttactgac ccagataatt tcatcgtctt
    28801 cgtcttcgat gtcatcaccg ggaacgtcgt cgagccagtc ttcgggctgc ttagtcatct
    28861 caggctcctt aaaaaaagag gctaatgtta ccagttaaga tgcgcactga aaaacggttc
    28921 tctgttagac ttcagagaaa ctctctacat tatggcactt gcaatgaaag taatctctca
    28981 agttgaagcg cagcgcaaga ttctggaaga agcagtttcc actgcgctgg agttggcctc
    29041 aggcaaatcg gacggtgcgg aagttgccgt cagcaagacc accggcatta gcgtaagcac
    29101 gcgttatggt gaagtggaga atgttgaatt caatagcgat ggcgcgctgg ggatcactgt
    29161 ttatcaccag aaccgcaaag gtagcgcatc atccaccgat ttaagcccgc aggccattgc
    29221 ccgcaccgta caggcggcgc tggatattgc ccgttatacc tcgccagatc cctgtgccgg
    29281 cgtggcagac aaagagctgc tggcctttga cgcaccagat ctcgacttgt tccaccctgc
    29341 ggaagtttcc ccggatgaag ccattgaact ggcggcccgc gcagaacagg cggcattgca
    29401 ggcggacaaa cgcattacca ataccgaagg cggcagtttt aacagccact acggtgtcaa
    29461 agtttttggc aacagccacg gcatgttgca gggttactgc tctacgcgtc attcactctc
    29521 cagctgtgta attgccgaag aaaatggcga tatggagcgt gattacgcct acaccattgg
    29581 ccgcgcgatg agcgatctgc aaacgccaga gtgggtcggg gccgactgtg ctcgccgtac
    29641 tttatcgcgc ctgtcaccgc gtaaactctc caccatgaaa gcgccagtca tttttgccaa
    29701 tgaagtggca accgggcttt ttggccatct ggtgggggcg atagcgggtg gctcggttta
    29761 ccgtaaatct acctttctgc tggattcgct gggtaaacaa attctgccgg actggctgac
    29821 cattgaagag catccgcatc tgctgaaagg gctggcgtcg acgccattcg acagcgaagg
    29881 tgtgcgcacc gagcgtcgcg atattattaa agatggcatc ctgactcagt ggctgctgac
    29941 cagctactcg gcgcggaaac tggggctgaa aagcaccgga catgcgggcg gtattcacaa
    30001 ctggcggatt gccggacaag ggctaagctt cgaacaaatg ctcaaagaga tgggcaccgg
    30061 gctggtggtg acggagctga tgggccaggg cgtgagtgcc attaccggtg attattcccg
    30121 tggtgcagcg ggcttctggg tagagaatgg cgaaattcag tatcccgtta gtgaaatcac
    30181 catcgcaggt aatttaaaag atatgtggcg caatattgtc accgtcggta acgatattga
    30241 aacacgcagt aatatacagt gtggttctgt gctgttgccg gagatgaaaa tcgccgggca
    30301 gtaaaaaact ggcgcgacct gccgcgctat aaactaaaaa attacacaaa atcattcgca
    30361 ctgcatcgag acggaaagtg aatgagggcc ggggagcgta ctcgcagtac gtgacccggg
    30421 cgaatgagcg tagccgtcga tgaggcagtg tgaaggatga agtgtaaata aaaaaggaag
    30481 tgagcaatgc gtaaaagcct gttagctatt cttgcagtct cctcgttggt attcagttct
    30541 gcgtcgtttg ccgctgatct tgaagacaat atggaaaccc tcaacgacaa tttaaaagtg
    30601 atcgaaaaag cggataacgc ggcgcaagtc aaagacgcgt taacgaagat gcgcgccgca
    30661 gcgctggatg cgcaaaaagc aacgccgccg aagctcgaag ataaatcacc ggacagcccg
    30721 gaaatgaaag atttccgcca cggtttcgac attctggtcg gtcagattga cgacgcgctg
    30781 aagctggcaa atgaaggtaa agtaaaagaa gcgcaggctg ctgcagagca actgaaaacg
    30841 acccgcaacg cctatcacca gaagtatcgt taattcctca tttccctgtt gcctgcactc
    30901 aggtaacagg gaacttatca tcgcaaatga tgcaccacct gattgctgct gccgcgccag
    30961 atcagggaag ggtcttttaa atcctgcaca aatttgccgt cgaccagtac gttaatcaaa
    31021 tcaacaacct gcatttgcgc agcgttgagt tcgtcgagtt tatagcctgt ccacacccag
    31081 atgtctttac ccggacactc ggcgcggatg cgttttacca gtttcagaat atccggcacg
    31141 ttttgcggat gcagcggatc gccgccggag agggaaatac cctggcgttt gatacgagtg
    31201 tcattcagat cgttaataat ctggtcttcc attgctttag taaatgtctg accggaattt
    31261 acccgccatg tgcttttgtt atagcaaccg gggcattcat gaacacaccc ggagacaaac
    31321 agggtgcagc gagtgccggg gccgttgacg atgtcgacag gatagtactg atgataattc
    31381 attgtttcgc ctggcttatc agtccgcgta taggatgcat ccggtaatca acgcctgatg
    31441 cgacgctgtc gcgtcttatc agacctacaa tcgttgttgc catgtaggcc ggataaggcg
    31501 tttacgccgc atccggcatt tgcttaacgt agagtgaaga ttaacctatc tgcccattcc
    31561 ccaaatgttt aacgcggcgc ttaacttctt cctgcttacc ggcgttaaat ggacgtgcat
    31621 ccgggctacc taaatatccg cacacgcggc gagtcaccga cacacgggag gcgtcatggt
    31681 taccacattt cgggcaagtg aagcctttgc tggtgcactc gaactcaccg gtaaagccac
    31741 actcgtagca ctcatcaatc ggtgtattgg tgccgtaata cggtacatgc tgatagctgt
    31801 aatcccagac atcttccagc gccttcaggt tgtgctgaat gtttggatac tcgccgtagc
    31861 aaatgaaacc accgttcgct agcggcgggt aaggcgcttc aaagtcgatc ttgtcgtacg
    31921 ggttcacctt cttctccaca tcgaggtgga aactgttggt gtagtaacct ttatcggtca
    31981 cgcccggcac cacgccaaac tcagcagtat cgagacggca gaagcggtcg cacaggtttt
    32041 cactcggcgt gctgtagaga ctgaaaccat aacccgtttc ttctttccac tgatccactg
    32101 cctggcgcag acgttcaaca atggcgatac ctttcgcgcg aagctgctcg ttgtcgtaaa
    32161 catgctcgcc gccgaacagc gcgttaatgg tttcgtggat gccgatgtaa cccagcgaaa
    32221 tagacgcacg accgtttttg aagatttcag aaacatcatc gtcagcattc agacgtacgc
    32281 cacaagcacc ttccatatag aggatcgggg cgacgcgcgc tttcacgcct tcgagacgag
    32341 cgatacgggt catcagtgcc ttacgtgcca gcaccagacg ttcatccagc agcttccaga
    32401 aggtggcttc atcgcctttt gcttccagag caatacgcgg caggttcagg ctgatcacgc
    32461 cgaggttgtt acgaccatcg tggatctgct cgccgttttc attttcccac acgccgagga
    32521 agctgcggca gcccatcggg gttttaaacg aaccggtgac tttcactacc tgatcgtagt
    32581 tcaggatatc cggatacatg cgcttgcttg cgcactccag cgccagctgt ttgatgtcgt
    32641 agttcggatc gcctttttta tggttcaggc catcgcgaat cgcaaacacc agtttcggga
    32701 acaccgcagt tttacggttt ttaccaaggc ctgcgatacg gttacgcagg atagattcct
    32761 gaatcaggcg cgactcccag ctggtgccca gaccaaaacc aaaggttaca aacggcgtct
    32821 gaccgttggc ggtgtgcagg gtgtttactt cgtactccag cgactggaag gcatcgtagc
    32881 actctttaat ggtacgagag ttagcgtagc cttcggcgtc cgggatgctc cactcttctg
    32941 cggtcttgcg atgtttgttg tagctggcgg tgacaaacgg tgccagcact tcatcgatac
    33001 ggttaatggt ggtgccgcca taaatatggc tggcaacctg agcaataatc tgcgcagtta
    33061 ccgcggttgc cgttgagatc gacttcggcg gttcaatctc ggcgttcccc attttaaagc
    33121 cctgggtcag catgcctttc aggtcgatca acatgcagtt gaacatcggg aaaaacggtg
    33181 agtaatcgag atcgtgatag tgaatatcgc cacgctcatg tgcctgcacc acgtcacgcg
    33241 gcagcaggtg ctgacgtgca tagtgtttag ccacgatccc ggccagcagg tcgcgctggg
    33301 ttggaatcac cttgctgtct ttgttggcgt tttcgttgag taacgaggcg ttggtctgct
    33361 cgaccagacc acggatctcc tggttcaggc gaccgcgttt ttcacgttca atgtcgcgat
    33421 cgtgacggta ctcgatgtaa gcgcgagcca gttgtttgta tggacccgac atcagctggt
    33481 tttcaactgc ggtctggatc tcgttgatat ccacctggtt gcggccctgc atctgctcgc
    33541 tgacaaccgc ggcaacagtg gcgcaataat cggcatcatc gacttccgct gctttagctg
    33601 cacgcagaat cgcttctttg atgcgctctg atttaaacgg cactttgcag ccgtctcgtt
    33661 tcatcacatg cggtgtcatg atcgctccat atttttaaga acaggttatc cacagaaatt
    33721 gggaaaggcg ttttccggtt ttttcgtttc gttttccggt gctttccgca atcgccatcc
    33781 gcttatccac aacgccgcac ctttttcgtg gggagctgta gtagcaatta tagtcgatta
    33841 atacaacata ttgggttggg acgcatttta aagtctatat atagtgcttt gcatcaagga
    33901 tgttttaact ttttttgatg tagctcaaag taaaaagcag agcgtacgga tgacgggcgc
    33961 tacagcgtta tgtaaatttt ttaatgaatt tgctggttga aaaatcaaca aaaacaacat
    34021 gctgacagac aaaaccccgg aatgaccggc attaccgggg cttagggaag atttacttct
    34081 gtaaccacca gacagcctca aaaggccgta aattcatggc acagggttgt ggtgaggctt
    34141 cttcgtagtt atgcatcaca agctgccagt tgccgcgcat ttgccctggc tgccagggtt
    34201 ggatctcacg gctaaggttg gcaatgacca gcaaggtttg ccccttccat tcacggcgat
    34261 agcaccacaa tacagggctg tttggcagca gatcctggta attgccccat gtcaggatgg
    34321 cttcttgctt acgcagtgcg attaactttt ggtaggtgta aaacaccgag gactcatcgg
    34381 ccagcgcggc ttctacgttg atttgttgat agttatcgcc caggccaatc cacggttcgc
    34441 cagccgtaaa cccggcatta tcgccgttgc tccattgcat gggcgtgcgg ctgttgtcac
    34501 gggatttact ggcgaggatt gccaataact cgtcggcatc acgaccatcg ttgcgcagct
    34561 cggcaaacat attgaggctc tccacgtcgc gatagtcagt aatgcgcgtg aaatgcgggt
    34621 tggtcatgcc aatctcttcg ccctggtaga tatacggcgt tccctgcatg ccatgcagca
    34681 ccatcgccag catttttgcc gcaggcacgc ggtattcacc ttcatcacca aagcgagaaa
    34741 caatgcgcgg ctgatcgtgg ttacaccaga acaaggcatt ccatgccacg ttgtgcattc
    34801 cttgttgcca gtggcggaac aatgttttca acgccacaaa gtcaggttta gccagcgtcc
    34861 atttttcacc accgggataa tcgaccttca ggtgatgaaa attaaaggtc atcgacaatt
    34921 cactgcctgt cagtgccgcg tatcgctggc aatgctcaag gctggtggag gacatttcac
    34981 ctacggtcat taacccgcgt ggcgtaaaca catcgcggtt catctcgtgc aaaaactcgt
    35041 gtgctcgtgg cccgtcggtg tagaagcgac gcccgtcgcc atccaggtct tcagggaaac
    35101 gcgggtcttt ggagatcaga ttcaccacat ccaggcgcaa cccgtcgacc ccacgatcgg
    35161 cccagaattc acagactttt ttcagctctg cgcgtaccgc tggattctcc cagttgagat
    35221 ccgcctgttc tggtgcaaag agatgcaaat agtactgttc gctttccgca tgccagcgcc
    35281 acgcactacc gccaaatttt gaacgccagt tgttcggtgg cgtttctggt tcgccatcgc
    35341 gccagatata aaactggcgg taagggcttt ctttgttcag cgcctcgcga aaccaggcat
    35401 gttgggtaga ggtatggtta aacaccatat cgagaatgat acgaatcccg cgcgattttg
    35461 cctgcgtcac cagttcgtca aaatcgtcca gcgtgccgta ggtgggatca atcgccgtat
    35521 agttcgctac atcgtaaccg ttatcgacct ggggagagac ataaaagggg gttagccaga
    35581 tggcatcaac gcccagttta tgcagatagt ccaggcgttg gataacgcca cgtaaatcgc
    35641 cggtgccgct acccgtggtg tcctgaaaac tctttggata aatctggtag ataacgccgt
    35701 tttgccacca gtggggaaga ttagtcatta cgttattcct gcaaatgcga gggggcgcaa
    35761 ttgcgccccg aagaaaatta aacaatgtcc agcgtgccca ggcggtattt ccgctgatag
    35821 ataaacgagg tgagtacaat cgggatgatg atggcgatag ccattgccag cgcaaatacc
    35881 tgccagtagc tcggttgaat cgagagaatt cccggcaggc cgcctacgcc gatgccattc
    35941 gccataacgc cgttcaggcc gcatagcaat cctgccagac cagaaccaat catcgcgcac
    36001 agcatcggga agcgatattt caggttgatg ccgtacattg caggctcagt gaccccaagc
    36061 caggcggaga tagcggcagg cacggagatc tcgcgttcat tgtgcttgcg gctggaaatg
    36121 ataatgccta tcacggcgga gccctgagcg atattcgaca gcgcaatcag cggccacact
    36181 ggcgtgccac ccatgctttg aatcatctgc aaatcaatag caagggtggt ctggtgtacg
    36241 ccggtgatca ccagcggcgc gtacaggaag ccaaacaatg ccgcaccaat cggagcaaag
    36301 ttgccggtca tcaggtgacg taccgcaaag gcaacgccat cgccaatcat gcgaccaaac
    36361 ggaccaatca gcgcatgggc gaggaacacc gcgaggatca gcgaacagac gggtaccacc
    36421 accagataga ggtaatccgg cacgatgcgt ttaaggcgag tttcaataac gcccagcgcc
    36481 agcccggcta acagtgccgg gatcacctgc gcctgatagc ctactttggc gatgctgaac
    36541 atgccaaagt cccacacttc cggcagctgc tgcccgagca gataagcgtt catcagctgg
    36601 ggagaaacca gtgtcacgcc aagcacgata ccaaggatcg gcgtgccgcc catttttttc
    36661 accgctgacc agcaaatacc gaccggcagg tagaagaaga tcgcttcacc gatcaaccac
    36721 agaaaatcgt agatcgtttg cagggaaggg tacatttgcg ccagcgtctg accgttgctc
    36781 atgggcaaat cgccgatcac attgcgaaaa ccgaggatca aaccgccgct aatcaacgcg
    36841 ggcagcagcg ggaagaagat ctccgcgaaa tgagagatca actgctcatg ccatttcata
    36901 ttctgccggg cggctttttt tacctgctct ttatcaacct gcgcctgtcc ggttgacgca
    36961 atcagtgctt gatagtaatc acccacattg gtgccaatca ccacctgaaa ttgcccggca
    37021 ttggtgaaac agcctttcac catggggagt tgctcaattt ctttcggtct ggcattggcc
    37081 ggttggttga ggacaaagcg caggcgagta atacagtggc tcaccgtcgc aatattgccg
    37141 cgcccgccga ccagttcaat caaccgatcg atatccgttt ggtttatttt gctcatcata
    37201 aagccccatg gcagatgaca tttttggttg gctgcagaat atattgcgcg gaaaaattta
    37261 aaaacgggaa cgttcccgaa acgcagcgaa gatcacaatt tatcgttcag gaaacgatca
    37321 ggacagggtg gcggggatga tgatttgttg cggttcgctg cgcccggtta cctgtgcgat
    37381 caattggcag gccgcctggc gtccagcttc ggcgtaaccg ggatctacgg tgacgatctc
    37441 cggatggagg aatttcatta acggtgtatt accgacgctc gccagttgca aggtgtcgat
    37501 gcgttgctct tgcaggtatt tacttgcgcc aagtgccagc gtgtcggttg cgcacagtaa
    37561 cgcagtagtt tcaggcgtaa tcacttttgc cacgttctca tagccttgct tcatagcaag
    37621 ccctggcaga gcggcaacgg gatgcagttt atgcgctttg cagaacgcca ggtaggcttc
    37681 gtgacgtcgc ttaccggttg tcacgtcact atgcggcacg ccgagataac tgatattacg
    37741 atgcccctgg tcatacagcc gttgcatcag gatcttgatt gccccttcgt cgtcataaca
    37801 gaccgaagca aagccttttg cgtcacgcgc cagcagaacc agcgatgact gccagtgggc
    37861 taacatttct tctgttatgc cagtaaaacc gaacagcact acgccgtcga tattacgccg
    37921 tttcagcacc cccaaatgtt cggcaactaa ttgtggggaa aactgacttt ccatcatgat
    37981 tgggtcgtaa ccttgttcat agaacgctgg cagcatggtt tgaacggcga gattttctga
    38041 caacgaatcc agacgggtaa caatgatggc gaccacttta tcgctttgcc cacgcatagc
    38101 gcgcgcagag cgggaagggg aaaatccatg ctgattcatc actgcttcaa cacgctcgcg
    38161 ggtgcgctgg ctcacgccgc tttcgttatt cagcacccgg gaaactgtag atttccccac
    38221 gccgcttaag cgtgcgatgt ctttgatggt cagccgattt tgcatcctgt tgtcctgtaa
    38281 cgtgttgttt aattatttga gcctaacttt acccgtgcat tcagcaatgg gtaaagtctg
    38341 gtttatcgtt ggtttagttg ccagcaggta ttatatcgcc atagatgcta cgaatattat
    38401 tggattctcc ttattatttg cggcgctttt ttcacttacc ggaggttata tggaacctga
    38461 tcccacgcct ctccctcgac ggagattaaa acttttccgg taagcccgtc ttttcacggc
    38521 gttaccggat gcgtaaggcc gtgacgtttt aacgtccctg ctcagcttta ttaccttcag
    38581 gtaaggcttc gccacgcctg aagacatttc tgtactgttt cagacagtgc ggagggactc
    38641 cttatgttta aagaaatttt tacccggctc attcgccatt taccttcccg tctggttcat
    38701 cgcgatccat tgcctggcgc gcagcagaca gtgaatacgg cggtcccgcc gtccttaagt
    38761 gcgcattgcc tgaaaatggc ggtgatgccc gaagaagaat tatggaaaac gttcgacacc
    38821 catccggaag ggttaaatca ggcggaagtg gaatctgccc gcgaacaaca tggtgaaaat
    38881 aaattacccg cacaacaacc gtcgccgtgg tgggtacatt tatgggtctg ctatcgcaac
    38941 ccatttaata ttttactcac cattctcggc gctatttctt acgccacgga agatttattt
    39001 gccgcgggcg ttatcgcgct aatggtcgct atttctacgt tgctgaactt tattcaggaa
    39061 gcacgttcca ctaaagcggc agatgccctg aaagcgatgg tcagcaatac tgcgacggtg
    39121 ctgcgcgtaa ttaacgacaa aggcgaaaat ggctggctgg agatcccgat cgaccagctg
    39181 gtgcccggcg atattataaa actggcggcg ggggatatga tcccggcaga tttacgtatc
    39241 ttgcaggcgc gggatctgtt cgtcgctcag gcgtcgttaa ccggtgagtc tctgcccgta
    39301 gaaaaagcag ctaccactcg ccagccggag cacagcaatc cgctggagtg cgacaccctg
    39361 tgttttatgg gcaccaccgt ggtgagcggc acggcacaag caatggtgat tgctacaggt
    39421 gccaatacct ggtttggtca actggcgggg cgtgttagtg agcaggaaag cgagccgaat
    39481 gcctttcagc aagggatcag ccgcgtcagt atgctgctga ttcgctttat gctggtgatg
    39541 gctccggtgg tgctgttaat caatggttac accaaaggcg actggtggga agcggcactg
    39601 tttgcgcttt cggtcgcggt aggcttaacg ccggaaatgt tgccgatgat tgttacctcg
    39661 acgctggcgc gcggcgcagt aaagctgtcg aaacagaaag tgatcgtcaa acatctggat
    39721 gctattcaga actttggcgc aatggatatt ctgtgcactg ataaaactgg caccctgaca
    39781 caggacaaaa ttgtgctgga gaatcatacc gatatctccg gtaaaaccag cgaacgcgtg
    39841 ctgcatagcg cgtggttgaa cagtcattat cagaccggac ttaaaaacct gctcgataca
    39901 gcggtgctcg aaggtacgga tgaagagtca gcgcgctcgc tggccagtcg ttggcaaaaa
    39961 atcgatgaga ttccgtttga tttcgagcgt cgccggatgt cggtggtagt ggcagaaaat
    40021 accgagcacc atcagctggt ttgcaaaggt gcattgcagg aaatcctcaa tgtgtgttcg
    40081 caggtgcgtc acaatggcga gattgtgccg ctcgatgaca tcatgctgcg taagattaag
    40141 cgggttactg atacgctgaa tcgtcagggg ctgcgcgtgg ttgcggtggc gacgaaatac
    40201 ctgccagcgc gtgaaggaga ttaccagcgg gcggatgaat ccgacctgat cctcgaagga
    40261 tatattgctt tccttgatcc gccgaaagag acaactgctc cggcactgaa ggcattaaaa
    40321 gcgagtggaa ttaccgtaaa aatccttact ggcgacagtg agttagttgc agcgaaagtg
    40381 tgccatgaag tggggctgga cgcgggagag gtggtcattg gtagtgatat tgaaacgcta
    40441 tctgacgacg aactggcaaa tctcgcacag cgtaccacgc tgtttgctcg tctgacgccg
    40501 atgcataaag aacgcattgt gaccttactg aagcgcgaag ggcatgtggt tggctttatg
    40561 ggcgatggta ttaatgatgc gcccgcctta cgcgctgcgg atatcggcat ttctgtggac
    40621 ggcgcggtag atattgcccg tgaagcggct gatatcatcc tgctggaaaa aagcctgatg
    40681 gtgctggaag agggggttat tgaaggacgt cgcactttcg ccaacatgct gaaatacatc
    40741 aaaatgacgg cgagctccaa cttcggtaat gtgttcagcg tgctggtggc gagtgctttc
    40801 ttgcccttcc tgccgatgtt gccgttacac ttgcttattc agaacctgct gtatgatgtg
    40861 tcacaggtgg cgatcccgtt tgataacgtc gacgacgagc aaattcaaaa gccgcagcgt
    40921 tggaacccgg cggatctggg gcgctttatg atcttcttcg gaccgatcag ctcgatcttc
    40981 gatattctga cgttttgcct gatgtggtgg gtattccatg ccaacacgcc ggaaacgcaa
    41041 acgctgttcc agtcgggatg gtttgtggtg ggcttactgt cgcaaacgct gattgtgcat
    41101 atgatccgca cccgccgtgt gccgtttatt cagagctgtg catcgtggcc gttaatgatc
    41161 atgaccgtga tcgtgatgat tgtcgggatc gcattgccgt tttcaccgct ggccagttat
    41221 ctgcaattac aggcgctgcc gttaagctat ttcccgtggc tggttgcgat tctggcaggg
    41281 tatatgacat taacccagtt ggtgaaaggg ttctatagcc gtcgttacgg ctggcaataa
    41341 agaataaact gggcacgata gccccgattc agattgcaga caaagtaagc tttgttcatg
    41401 ccggatgcgg cgtaaatgcc ttatccggcc aacaaattca ataaattgca ggaatcacgt
    41461 aggcctgata agcgtagcgc atcaggcgat tttgcgtttg tcatcagact taatccgggc
    41521 atgatagccc ggatttccat caagattagc gacgaacagc gatcgcttcg atctcaatct
    41581 tcacgtcttt cggcagacgg gcgacttcaa cgcaagaacg tgccgggaag gtggcgttgt
    41641 gttcggtgaa gaaggcttcg taggtagcat taacggtagc gaagtcgttc agatctttca
    41701 caaacacggt ggttttaacg atgtcgccca ctttcaggcc agcggcttcg acgatcgctt
    41761 ttacgttatc cagcgactga cgtgcctgtg cagcgacgtc tgccggtact tcacccgttt
    41821 tcggatttac cgggatctga ccggaggtga tgatcatatt gcccagatca acgccctgta
    41881 cgtaaggacc gatagctgcc ggtgcatttt ccgtcgcgat agttttgctc atgatttctc
    41941 ctttattaca gcggtaaaaa gtctggctca ttatagggag ccagactttt attaccaacc
    42001 gcaattaatt ggccagcacc acattatggg aaaactcttt ttcacagtat ttgcatttga
    42061 gcgcgatgtc attggcgcgt tttcgcacgg caaagctgga agaaaccggt tcggcatggc
    42121 tgatacagtt gctgttcggg cagaccagca cattgtcgat gcgctctggc agactggggc
    42181 gcgatttacc caccacttca tagttgtcga tacggttaac cgtggcttgc ggcgcataca
    42241 atgccagttg atctacctga tcttcactca aaaaggtatt ttcgattttg atcagatctt
    42301 tgcggcccat ctcgccagaa ggcaagttca gaccgatggt gatgcgctga tccgtttcgg
    42361 tcagcttgaa cagactcaac agcttaaaac cgatctgggc ggggatatgg tcaattaccg
    42421 tgccgcgttt aatagcttca acctgcaatt tattatcgtg tgtcatctct atttcccctt
    42481 acagtaccag atcgcgattc agaaccagtg ccagtaacgc ctggcgagcg aaaatcccgt
    42541 tgcctgcctg ctggaagtac caggcgtgtg gcgttttatc cacatcggtg gcaatctcat
    42601 caacacgcgg cagcggatgc agcactttca tattggcttt ggcgttgtgg agatcgctgg
    42661 cgcgaagaac aaactgcgct ttcacgttgg cgtactcgga cgggtccaga cgctcttttt
    42721 gcacgcgggt catgtacagg atgtctactt ccgccatcac ttcttcaata gagctgtgca
    42781 gactccatgc gatccctttt tcatcgagca tatccagaat gtattgcggc attgccagcg
    42841 cgtccggcgc gatgaagtaa aaacggttgc cgtcgaactt cgctaacgcc tgagtcaggg
    42901 agtgaacggt gcggccatat ttcaggtcac caaccattgc gacgtggaga ttgtccagac
    42961 gcccctgggt ttcctgaata gtgaataagt ccagcaaggt ttgcgtcgga tgttggttgg
    43021 agccatcacc ggcattcagt accggtacat tgccggaaaa ctcggtggcc aggcgcgccg
    43081 caccttcctg cggatgacgc atcactatcg catcgacgta agtgctgata accgaaatgg
    43141 tatcggccag cgtttcgccc tttttaccca gtgatgtatt ggcgctgtcg gagaagccca
    43201 ccacgctggc acccaggcgg tgcatagatg tttcgaaaga gaggcgggta cgggtagagg
    43261 cttcgaagaa acagctggca atgactttgt gcttcaacag ctctggttgc gggtttgctt
    43321 tcagtttcgc cgctgtcgcc agcaccagat taaggtcatc gcgactaagg tcgtttatgg
    43381 aaatgatatg tttctgatat agcggattag ccatctttta tctcctgacg cctgggcaaa
    43441 aaaaagcccc tcgattgagg ggctgggaat gggtgatcaa cgggaagaaa aacggcaggc
    43501 cagcgtcttt tttcagacgc ggtaagacaa aatgtcgaac acactgaacc atacatcctc
    43561 ccggcaaatt gtccggcatt atactcatag ccagaagcgg tgcaagcatt tgatgcaata
    43621 ttttgtcagc gcaaacggtt tatttgaatt aaaagtcaaa atgtatgcat ttttatttgc
    43681 tttgattctg gaagagaact gtcgttcggt atcaataatt gcagacattt cctgcttttt
    43741 tctttttcac tttcacgcaa tcagatatgc attttattcc attcatccga cttatacggc
    43801 ggagtgtgaa aacgaacggc taacactatt gcttacagct cagggatgcg cgctatcact
    43861 ttaatttcaa aatcaaagcc tgccagccag gtaacgccca ccgccgtcca gtttggataa
    43921 ggaggggcgc taaatatttc atttttgacc gtcatgatgt cttcaaattg tttttccgga
    43981 tcggtatgaa agctcgtaac atcaatgata tcgtcaaaag tgcatcccgc agctgccagg
    44041 gtcgcatgca aattatcaaa tgccagtctg acttgttgct gaaaatcggg ttctggtgtt
    44101 ccgtcctctc gacttcctac ttgcccggaa acaaacagca aatcgccgga acgaatagcc
    44161 gccgaataac gatgttcagc atacagtgaa tgtcggccag cagggaaaac agcggttctt
    44221 tctaccattt ggttatcctc aagatttacg acatgaacag aagatttata tttaccggga
    44281 gccgctttta gggaacgacg tgagtaaaca aaacccagac atcatggata atggctgggc
    44341 ttaattgagc gtagtcggtt atgccccaaa cgcgccatca atggtatgca tcgcgccggt
    44401 aacaaaactg gcttctggcc ctgctaacca tgcgaccatt ccagcgacct cttccggttg
    44461 cccatgtctt ttgatagcca taaaactatg caacatatcg cgcattggcc cgttggcggg
    44521 attagcgtcg gtatctattg gccctggctg gacgacgttg atggtgatcc cacgcggtcc
    44581 aaaatcacgg gccagcccgc gcgccatgcc ttgcagggca gatttactgg cggcataagc
    44641 agccatgcct gcaacaggca tacgatcgcc attcacggag ccgatgatta agatgcgccc
    44701 gccttcgggc atctgccggg cggcttcaac agaggcatgg taaggggcat gaatattgat
    44761 tttgaaaagg cgatcaatat cgtcggcatt taattccagg gcatcgccaa agacgccaat
    44821 acctgcattt accaccagga tatccaatgc gccgctctta cgaacgacat caataacagc
    44881 gtctctgtca gcactatctg tgaatactgc tgtcgctcca gtctcatgtg ccaggcgttc
    44941 agcggcatct ttcgaccccg cataggtgaa tcgtacattg gctccatcgg tgacgaaacg
    45001 acgtacgata gcggcaccta ttccacggct accaccgagg atgagaactg tcttacctgt
    45061 aaaagcgccc ataaggactc cttgatttat tatgtaacat gcattacaaa actgttttaa
    45121 ctttctgtca acaagttttg taatggtcac taaaaaacaa tctcgcgttc caggtcgtcc
    45181 cagacgtttc gctcctgagc aggcagtctc tgcggcaaaa gtgctttttc accaaaaagg
    45241 tttcgatgct gtcagtgttg ctgaagttac tgattatctt ggtattaacc ccccgagtct
    45301 ctacgcggct tttggcagta aggctgggtt atttagccgt gtactcaatg aatatgtcgg
    45361 tacggaagct attccgcttg ccgatattct tcgtgatgat cgtccggtag gggagtgcct
    45421 ggctgaggta ttaaaagaag cggcgcgcag atatagccaa aacggcggct gcgctggctg
    45481 tatggttctt gaaggcattc atagtcatga tccacaagcg cgtgatattg ccgttcaata
    45541 ttatcacgcc gcagaaacga ccatttatga ctatatcgcc aggcggcatc cacaaagcgc
    45601 acaatgtgtg actgatttta tgagtaccgt gatgtcaggg ctttctgcga aggcacgaga
    45661 ggggcactcc ctcgaacaac tctgtgcaac agctgcactg gcgggggaag cgataaaaac
    45721 tattctcaag gagtgatgct gaccttgatc cgaaaggcgg gaacgcgcct gccgataagt
    45781 tgtgataaga caataattca cgcgttaagg ctggcggaat tgatcatctt ttcgtataac
    45841 ggtggaaatg aaacattgtt ttaattaagg agtggaaaag atgatcatcg gaaatattca
    45901 taatcttcag ccatggctac cccaggagtt acgccaggcg attgagcata tcaaagcaca
    45961 cgttacggca gaaacgccaa agggtaagca cgatatcgaa ggcaatcgtc tgttttatct
    46021 tatctcggaa gatatgaccg agccgtacaa agctcgccgt gcggagtacc atgcccgtta
    46081 tctcgacatt cagattgtgt taaaaggtca ggaaggcatg accttcagca cacaacctgc
    46141 aggtacgccg gagaccgact ggttagtaga taaagacatc gcatttttgc cggaagtcgt
    46201 tgatgagaaa acagttatcc tgaatgaagg tgattttgtt gtgttttatc cgggggaagt
    46261 gcataaaccg ctgtgcgcag tgggtgcacc agcccaggtt cgcaaagcag tagtgaagat
    46321 gctgatggcg tgatgacttt tcgccgtaaa taactccagg tttacggcga gtttgtgaaa
    46381 agagcgattt ttgatatttt tttgtgagta aaatttgtaa tgcttagacg ttcttattaa
    46441 ctcaaggagt tcgtcatggc aaaaatatca gatttgaatt gttatcaaca catggcatca
    46501 gccgacaatg ttaaaccaaa aaatgaagtc ttaaatacct ggtgtgtggg aatgaatgat
    46561 tttgctcgta atgccgaggg gcaggatagc agaaggaata ttctatctcc tggagcattt
    46621 ttggagtttt tggcaaaaat atttaccctg ggttatgtgg attttagcaa atgctccaac
    46681 gaagcgggta gaaatat
//
LOCUS       DACADF010000018        44993 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.018, whole genome shotgun sequence.
ACCESSION   DACADF010000018 DACADF010000000
VERSION     DACADF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.018"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..4785
                     /locus_tag="ISB30_001006"
     CDS             <1..4785
                     /locus_tag="ISB30_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HAP1988667.1"
                     /translation="QIDGDKAIVNNDGDNAISNGGTGTQINGDEATVNNNGNTTVDGK
                     DATGTEIAGNNAVVNQDGTLDVSGGAHGIDITGDGAIANTTGAITVADTDSVGIQVDG
                     NAVTVSNDGDSTITNGGTGTQVNGNDAQVNNTGNTTVDGKNSTGTEITGNNGTVNLDG
                     GLIVTGGAYGVEIAGDSAALNNEGDISVSDEGSIGVLINGEKATVSNTGDVNVSNNAT
                     GFDITTNEGNISLAGSMQVGDFSTGMDLNGNNNSVTLAAKDLNVTGQKATGVNVSGDA
                     NTINITGNVLVDKDQTADNAADYFYDPSVGINVNGSDNNVTLDGKLTVVADSELTTRG
                     SAGAIIFDGSQENITGLSIAGDGNTFRLNGGIQLVGEANKLTDGSTIASERKGSGKVP
                     LVSVDGKSSVHLNGDSTISGDVLLGNTQIIDLRNGAMLDVGADATINMQVDTYERYAM
                     SADHLIDASSGSQLVNNGDINLRSIGFAAITGEDSFGSNTGNLTLSQYLDGLFANGGY
                     GYLATKGASVVNTGTITTKVAEQESVINLGASLGFNDLVFYNAANSMTGLSAKTHGYV
                     LNENGGKIEMFGRGNVGMLAIDESTAENAGQITLDALWVDVNDTTTLRDNIGNDARGY
                     SVGMGVGTDAGSGPRKNATAVNQQSGVITVYNAGIGMAAYGAGNTVINQGTINLEKNG
                     NYDSSLGVNSLIGMAAYNSGTAINDQTGVININADNGQAFYSTGNGVILNYGTICVNT
                     NCLTGNDYNDTDSYTSILYTGGDVITAQSETQSLTKKATISDKKDGNVVNSGALSGAD
                     IAISSGKLVNTSTGTINNAIIINGGALSNEGSVAKVTQNAGTFNNTGSVNSRMTQTGG
                     TFNNQQGGTVKNGSRLANSAIANNMGIWSLGDTSSGSNASMLEINNNAVFKNSGDFIL
                     DNSKNAVHLNQSGTFYNTGKMLLSNSSHSGVLNFWGGNGRFINGGIADVTAKSLAVSA
                     SDAGSSNAFFWNQSNGVVNFDKDSGVAVKFIHSNYVAQNDGTMNISGNNAIAMEGDKN
                     ARLVNNGTINLGTSGTTDTGMIGMQLDSGTTADAVIENNGAINIYANNSFAFSMLGSV
                     GHLVNNGTVTIADGVTGSGLIKQGDSVNIEGVNGNNGNNSEVHYADYTLPDVPGSSVS
                     VAASTPSSDGSQNKLNGYVVGTSSDGSAGKLKVNNASLKGVSVNTGFTAGTSSTSVTF
                     DNVVQGSNLTDAETITSTSVVWNAQGTTDASGNVDVTMTKNAYTDVATDSSVNTVAQV
                     LDAGYTNNELYSSLNVGTTAELNSALKQVSGSQATTVFNEARVLSNRFSMLSDAAPEV
                     ANGLAFNVVAKGDPRAELGNDTQYDMMALRKSLTLTEHQNLSLEYGIARLEGNGSDTA
                     GDNGVTGGYSQFFGLKHQMAFDNGMNWNNALRYDVHQLDSSRSVAYGDINKTADANVK
                     QQYLEFRSEGAKTFELREGLNVTPYAGVKLRHTLEGGYQERNAGDFNLSMNSGSETAV
                     DSIVGLKLDYAGKDGWSANATLEGGPNLSYVKSQRTASVSGAGSQRFNIDDGQNGGGF
                     NSLATMGVKYSSQESALQVDAFHWKEDGISDKGVMLNFKKTF"
     gene            5005..5871
                     /gene="pdxI"
                     /locus_tag="ISB30_001007"
     CDS             5005..5871
                     /gene="pdxI"
                     /locus_tag="ISB30_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HAP1988668.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHLG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVATSLNATPMQVALAWLLQRSPNILLIPGTSSVGHLRENMASDELVLTDDV
                     LATLDSIAQKAG"
     gene            complement(5911..6516)
                     /gene="azoR"
                     /locus_tag="ISB30_001008"
     CDS             complement(5911..6516)
                     /gene="azoR"
                     /locus_tag="ISB30_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP1988669.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELITELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTEKGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            6717..10619
                     /gene="hrpA"
                     /locus_tag="ISB30_001009"
     CDS             6717..10619
                     /gene="hrpA"
                     /locus_tag="ISB30_001009"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAP1988670.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQQSAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQAADEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            10892..11692
                     /locus_tag="ISB30_001010"
     CDS             10892..11692
                     /locus_tag="ISB30_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP1988671.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNAESVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            11902..13341
                     /gene="aldA"
                     /locus_tag="ISB30_001011"
     CDS             11902..13341
                     /gene="aldA"
                     /locus_tag="ISB30_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAP1988672.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGENVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADIELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(13383..14384)
                     /gene="gap"
                     /locus_tag="ISB30_001012"
     CDS             complement(13383..14384)
                     /gene="gap"
                     /locus_tag="ISB30_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP1988673.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            14573..15103
                     /gene="cybB"
                     /locus_tag="ISB30_001013"
     CDS             14573..15103
                     /gene="cybB"
                     /locus_tag="ISB30_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAP1988674.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            15348..15521
                     /locus_tag="ISB30_001014"
     CDS             15348..15521
                     /locus_tag="ISB30_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988675.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(15593..15742)
                     /gene="hokB"
                     /locus_tag="ISB30_001015"
     CDS             complement(15593..15742)
                     /gene="hokB"
                     /locus_tag="ISB30_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAP1988676.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            16005..16448
                     /gene="trg"
                     /locus_tag="ISB30_001016"
                     /pseudo
     CDS             16005..16448
                     /gene="trg"
                     /locus_tag="ISB30_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(16486..17409)
                     /locus_tag="ISB30_001017"
     CDS             complement(16486..17409)
                     /locus_tag="ISB30_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1988677.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            17626..18969
                     /locus_tag="ISB30_001018"
     CDS             17626..18969
                     /locus_tag="ISB30_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP1988678.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDAASLARNPFRVFTSLLRLELIENELLRQKAAEILRQRDIFTPRCR
                     QLLQEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEKTYRALHKEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQACNHGDASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            19194..20849
                     /locus_tag="ISB30_001019"
     CDS             19194..20849
                     /locus_tag="ISB30_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAP1988679.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            20989..21213
                     /locus_tag="ISB30_001020"
     CDS             20989..21213
                     /locus_tag="ISB30_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAP1988680.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            21276..21815
                     /gene="rimL"
                     /locus_tag="ISB30_001021"
     CDS             21276..21815
                     /gene="rimL"
                     /locus_tag="ISB30_001021"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAP1988681.1"
                     /translation="MTETIKVSESLELHAVAESHVTLLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     PHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(21807..22787)
                     /locus_tag="ISB30_001022"
     CDS             complement(21807..22787)
                     /locus_tag="ISB30_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAP1988682.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAMSDFNDVIAGSAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTISDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRAKV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTIHVRGPKVINGEERITRTLLAGLL"
     gene            22911..23903
                     /gene="tehA"
                     /locus_tag="ISB30_001023"
     CDS             22911..23903
                     /gene="tehA"
                     /locus_tag="ISB30_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAP1988683.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLCLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            23900..24493
                     /gene="tehB"
                     /locus_tag="ISB30_001024"
     CDS             23900..24493
                     /gene="tehB"
                     /locus_tag="ISB30_001024"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAP1988684.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCAKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            24796..25464
                     /locus_tag="ISB30_001025"
     CDS             24796..25464
                     /locus_tag="ISB30_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAP1988685.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(25499..26710)
                     /gene="ydcO"
                     /locus_tag="ISB30_001026"
     CDS             complement(25499..26710)
                     /gene="ydcO"
                     /locus_tag="ISB30_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HAP1988686.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQELT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLAWLAAKAVAPRYAVIAAMIIGIVIVTAQGDVVTTDIVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            26763..27299
                     /locus_tag="ISB30_001027"
     CDS             26763..27299
                     /locus_tag="ISB30_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1988687.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESMPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            27372..29333
                     /gene="rlhA"
                     /locus_tag="ISB30_001028"
     CDS             27372..29333
                     /gene="rlhA"
                     /locus_tag="ISB30_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HAP1988688.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATNATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHIDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWHQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSVTHTLDGQFDEANNA
                     EKAMNNLKDGLTKLGQTIYYARNVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(29425..29655)
                     /locus_tag="ISB30_001029"
     CDS             complement(29425..29655)
                     /locus_tag="ISB30_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HAP1988689.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            29834..30070
                     /gene="hicB"
                     /locus_tag="ISB30_001030"
                     /pseudo
     CDS             29834..30070
                     /gene="hicB"
                     /locus_tag="ISB30_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            30149..31555
                     /locus_tag="ISB30_001031"
     CDS             30149..31555
                     /locus_tag="ISB30_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP1988690.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDIKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            31800..32945
                     /locus_tag="ISB30_001032"
     CDS             31800..32945
                     /locus_tag="ISB30_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP1988691.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLENPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVSGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPVAEGGKFVPYSRWTQDYIAIMGGR"
     gene            32963..33976
                     /locus_tag="ISB30_001033"
     CDS             32963..33976
                     /locus_tag="ISB30_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1988692.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDPLMAEKLCGMTGSFALRPEHIRLNTHGEMQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            33977..34918
                     /locus_tag="ISB30_001034"
     CDS             33977..34918
                     /locus_tag="ISB30_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1988693.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIIVRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            34908..35702
                     /locus_tag="ISB30_001035"
     CDS             34908..35702
                     /locus_tag="ISB30_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1988694.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            35724..37148
                     /gene="patD"
                     /locus_tag="ISB30_001036"
     CDS             35724..37148
                     /gene="patD"
                     /locus_tag="ISB30_001036"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HAP1988695.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVNAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDSE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(37245..37340)
                     /gene="yncL"
                     /locus_tag="ISB30_001037"
     CDS             complement(37245..37340)
                     /gene="yncL"
                     /locus_tag="ISB30_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAP1988696.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            37536..37709
                     /locus_tag="ISB30_001038"
     CDS             37536..37709
                     /locus_tag="ISB30_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAP1988697.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRLLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            37795..38028
                     /locus_tag="ISB30_001039"
     CDS             37795..38028
                     /locus_tag="ISB30_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HAP1988698.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTL
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(38029..38478)
                     /locus_tag="ISB30_001040"
     CDS             complement(38029..38478)
                     /locus_tag="ISB30_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP1988699.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(38475..38993)
                     /gene="mddA"
                     /locus_tag="ISB30_001041"
     CDS             complement(38475..38993)
                     /gene="mddA"
                     /locus_tag="ISB30_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAP1988700.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            39174..39242
                     /locus_tag="ISB30_001042"
                     /pseudo
     CDS             39174..39242
                     /locus_tag="ISB30_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase yncb"
     gene            39242..39397
                     /locus_tag="ISB30_001043"
                     /pseudo
     CDS             39242..39397
                     /locus_tag="ISB30_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(39474..41576)
                     /locus_tag="ISB30_001044"
     CDS             complement(39474..41576)
                     /locus_tag="ISB30_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAP1988701.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVATTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDMRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGINIAWRFE"
     gene            41818..42879
                     /locus_tag="ISB30_001045"
     CDS             41818..42879
                     /locus_tag="ISB30_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HAP1988702.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWIATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDTTNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTTDNKI
                     LSRKKLLDDGKEHFFINISLDTTNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(42992..44491)
                     /gene="ansP"
                     /locus_tag="ISB30_001046"
     CDS             complement(42992..44491)
                     /gene="ansP"
                     /locus_tag="ISB30_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HAP1988703.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQDIVFKPETAS"
BASE COUNT        11471 a        10875 c        11688 g        10959 t
ORIGIN      
        1 cagatcgacg gtgataaagc catcgtcaac aacgatggcg acaatgctat cagcaacggc
       61 ggcaccggca cgcaaattaa cggcgatgaa gccaccgtga ataataacgg taacaccact
      121 gttgatggca aagacgccac cggcacggaa atcgcgggta acaacgcggt ggtgaatcag
      181 gacggcacgc tggatgtcag cggcggtgct cacgggattg atatcactgg agacggcgcc
      241 attgcaaaca cgacaggagc gatcactgta gcggatacgg actctgtcgg tattcaggtt
      301 gatggtaatg cagtgaccgt cagcaatgac ggagacagca ccattactaa cggcggtacg
      361 ggcacgcagg ttaacggcaa cgatgctcag gtcaacaata ctggcaacac cacggtagac
      421 ggcaaaaact ccaccggaac agaaattacg ggcaataacg gcactgtaaa tctggatggt
      481 ggtctgattg tgacgggtgg tgcctatggc gtggagatcg ctggcgacag cgccgcgcta
      541 aacaatgaag gagatatttc ggtttccgat gagggctcaa ttggcgtact catcaacggt
      601 gaaaaagcaa ccgtatccaa cacgggtgat gttaacgtca gcaataacgc gacaggtttt
      661 gacattacca ctaatgaagg gaatatttcg ctggcaggca gtatgcaggt gggcgacttc
      721 tctaccggga tggatctaaa cggtaataac aacagcgtca cgctggcggc aaaagatctg
      781 aacgtcaccg gtcagaaggc gacaggcgtg aatgtatccg gcgatgccaa cactattaat
      841 atcaccggta atgttctggt agataaagat caaacggctg ataacgcggc agattatttc
      901 tacgacccgt ctgtggggat taatgtcaac ggtagtgata acaatgtcac tctggacggt
      961 aagttaacgg ttgttgctga cagtgaactt actacacgcg gttctgctgg cgccattata
     1021 tttgatggca gccaggagaa tatcaccgga ctcagcattg caggtgatgg taatactttc
     1081 cgtctgaatg gcggtattca actggttggt gaggcgaaca agctaactga cggttccact
     1141 attgcatccg agcggaaagg cagtggaaag gtacctcttg tcagtgttga tggaaaatcg
     1201 tccgttcatc tgaacgggga ttccactatt agcggtgatg tgctgctcgg caatacacaa
     1261 atcattgatt tgcggaatgg tgccatgctg gacgtcggtg cggatgccac gatcaacatg
     1321 caggttgata cttacgagcg ttatgctatg tcagccgatc acctcattga tgcatcgtct
     1381 ggctctcagt tggtcaataa cggtgatatt aatctccgaa gcatcggttt tgccgctatt
     1441 actggtgagg attcattcgg aagcaacact ggcaatctca cgctttctca gtacctcgat
     1501 gggctgtttg caaacggagg gtatggctat ttagccacca aaggggccag tgtcgtgaac
     1561 acaggcacta tcactacgaa agtggcggaa caagagagcg taatcaacct tggagcgtca
     1621 ctgggtttta atgatttagt cttttataac gcagcgaaca gtatgacggg ccttagcgcc
     1681 aaaactcatg gttatgtact taatgagaac ggcggcaaaa tcgagatgtt cggacgtggc
     1741 aatgtcggta tgttggccat cgatgaaagt acggcagaaa acgcggggca gattacactg
     1801 gatgcactgt gggttgatgt aaacgataca acaactttgc gtgacaacat tggtaacgac
     1861 gccagaggat attctgttgg tatgggcgtt gggacagatg ctggcagtgg accgaggaag
     1921 aacgccacag cagtcaacca acagagtggt gttatcaccg tttataacgc cggaattggt
     1981 atggccgcct acggcgctgg taatacggtt attaaccagg gcaccattaa tctggagaaa
     2041 aacggtaatt acgatagtag cctgggcgtg aattcgctta tcggtatggc tgcatataac
     2101 agtggtaccg ccattaacga tcaaaccggt gttatcaata ttaatgctga caatgggcag
     2161 gcattttata gcactggtaa cggtgtcatt cttaactatg gcaccatttg tgttaacact
     2221 aattgcctga cgggcaatga ctataatgat actgactcct atacctcgat attatacacg
     2281 ggtggcgatg tcatcactgc acagagtgaa acacaaagtc tgaccaaaaa agccacgatt
     2341 agcgacaaaa aagacggtaa tgttgttaac agtggtgcgc tttccggtgc tgatattgcg
     2401 attagtagcg gtaagttagt taatacatca acgggtacga ttaataacgc aataattatc
     2461 aacggcggtg cgttaagtaa cgaaggctct gtggcgaagg tgacgcaaaa cgccggtact
     2521 ttcaataaca cagggtcggt taacagtcgt atgactcaga caggaggaac gttcaataac
     2581 caacagggcg ggacggtgaa gaacggctcc agactggcca acagcgctat tgctaataac
     2641 atggggatct ggtcccttgg cgatacgagt agtggtagta atgccagcat gctggaaatt
     2701 aataataatg ccgtgttcaa aaacagtggt gacttcattc tggataatag caaaaacgct
     2761 gttcatctga atcagtcagg tacgttctac aataccggga aaatgttgct atccaactct
     2821 tcccatagcg gcgtactaaa tttctggggt gggaatgggc gctttattaa cggcggtatt
     2881 gcggatgtca cggcgaaatc tttggccgta agcgccagtg atgccgggag cagcaatgcc
     2941 ttcttctgga accagagcaa tggggtcgtc aattttgata aagacagcgg cgttgcagtg
     3001 aaattcattc acagcaatta cgttgcgcag aatgacggca ccatgaatat tagcggtaat
     3061 aacgctattg cgatggaagg ggataaaaac gcacgactgg ttaacaacgg cacgatcaac
     3121 ctcggaacgt cgggcacgac cgacacgggc atgatcggga tgcaactgga cagcggcacg
     3181 acagctgatg cggtcattga gaacaatggc gccatcaaca tttacgccaa caactccttt
     3241 gcatttagca tgttgggttc cgtaggccac ctcgtgaaca acggcacagt gactattgcc
     3301 gatggagtga caggttccgg gctaatcaag caaggtgaca gcgttaacat tgaaggtgtg
     3361 aacggcaata acggtaacaa tagcgaagtg cattatgctg attacacttt gcctgatgta
     3421 cctggctcgt cggtttctgt tgcggccagc accccgtcca gtgacggaag ccagaacaaa
     3481 ctgaatggtt atgttgtagg aaccagttct gacggcagtg ccggcaagct gaaagtgaac
     3541 aatgcaagcc tgaaaggggt ttctgttaac accggattta cggctggaac cagctccacc
     3601 tccgtgacct tcgacaacgt cgttcagggc agtaatctga ccgacgcaga gacgattaca
     3661 tcgacctctg tggtatggaa cgcgcaaggc actactgacg ccagcggcaa cgttgatgtc
     3721 acgatgacta aaaatgccta caccgatgta gcaacagatt catctgttaa caccgtcgcg
     3781 caggtactgg atgcgggcta taccaacaac gagttgtaca gcagcctgaa cgtaggcacc
     3841 acggcggaac tgaacagtgc gctgaaacag gtcagcggca gccaggcaac aacggtattc
     3901 aatgaagcac gagtgttaag taaccgcttc agcatgttgt ccgatgcagc accggaagtc
     3961 gcaaacggtc tggccttcaa cgtggtagcg aaaggcgacc cgcgtgctga actgggtaac
     4021 gacacacagt acgacatgat ggcgctgcgt aaatctctga cgctgactga acatcagaac
     4081 ctgagtctgg aatacgggat tgcgcgtctg gagggtaatg gttcagatac cgcaggcgat
     4141 aacggagtga caggcggtta cagccagttc ttcggtctga aacaccagat ggcctttgac
     4201 aacggtatga actggaataa cgccctgcgt tatgatgtgc accagctcga cagcagccgc
     4261 tcggtagcgt acggcgatat caataaaaca gccgatgcta atgtgaaaca acagtacctg
     4321 gagttccgta gtgaaggggc gaaaaccttt gagctgcgtg aagggctgaa cgtcacgcca
     4381 tatgccgggg ttaaactgcg tcacacactg gagggtggct atcaggaacg caatgcgggt
     4441 gactttaacc tgagcatgaa cagcggtagc gaaacggcag tggacagcat tgttgggctg
     4501 aaactggact atgccggtaa ggacggctgg agtgcgaacg ccacgctgga aggcggcccg
     4561 aacctgagct atgtgaaaag ccagcgaact gcgagcgttt cgggcgcggg aagccagcgt
     4621 ttcaacattg atgatggtca gaatggtggt ggtttcaaca gcctggcgac aatgggtgtg
     4681 aaatacagca gccaggaaag tgcgttgcag gtagatgcat tccactggaa agaagacggc
     4741 atcagtgata aaggtgtgat gctaaacttc aagaaaacgt tctaattttt agcatgtaat
     4801 gcataaaccg caacgctgat acaggttgcg gtttttttat tgccggatgc ggtgcttgac
     4861 gtgtcttaac ctgcctgggg tttcgtgtct ttcagggcaa cagtacaact catcccgcaa
     4921 tcttgtttac accctcgcat tatttatctc ttttcgtttc tatactgatt tttcttaatc
     4981 cgttttatta cagggcaggg tgcgatgagc agcaacacat ttactctcgg tacaaaatcc
     5041 gttaaccgtc ttggttatgg cgcgatgcaa ctggctggtc ctggggtttt tggcccccca
     5101 agagatcgcc acgtcgctat aaccgtgctg cgcgaggcgc tggcattggg cgtcaatcat
     5161 attgatacca gcgactttta tggtccgcac gtcaccaatc agattatccg cgaagcgctt
     5221 tatccttact ctgacgacct gacaattgtc actaaaattg gtgcgcggcg tggagaggac
     5281 gcatcctggt tacccgcatt ttctccggca gagctgcaaa aagcggtaca cgataatcta
     5341 cgtaatctcg gcctggacgt actggatgtg gttaacctgc gcgttatgat gggggatggt
     5401 catggcccag cggaaggatc gattgaggcc agcctgacag tgctggcgga gatgcaacaa
     5461 caaggtctgg taaaacatct tggcctgagt aacgtcacac cgacgcaggt tgcagaggcg
     5521 cgaaagattg ccgaaatcgt ctgcgtgcag aacgaataca acattgccca ccgtgctgat
     5581 gacgcaatga ttgatgcttt ggctcgtgat ggcattgcct acgtgccgtt cttcccgctc
     5641 gggggcttta cgccgctgca atcctccacg ctttccgatg ttgctacaag ccttaatgcg
     5701 acaccaatgc aggtggcgct ggcgtggctg ttacagcgtt caccgaatat tttgctgatt
     5761 cctggcacct cttcggtggg gcatctgcgg gagaatatgg cgtccgatga attagtgcta
     5821 acggatgacg tgctggcaac gctggacagt attgcgcaaa aggcgggtta aataaaaaac
     5881 ctcccacttt cgtgggaggc atcataaatc ttatgcagca acaatactgt cgatggctgc
     5941 ttttgcgtca gactgtgctt tcgctgccat ttccgggccg tacgcgatcc cttcggcgaa
     6001 gacaaatttc acatcggtaa tgccgataaa gcccaggaat gtggacagat atggcgttac
     6061 cagatccgtt gggccatctt tatggatccc accgcggctg gtgataacga tggctttttt
     6121 accggtgacc aggccttccg gacccttctc ggtatagcgg aaagtaacgc ctgcacgtgc
     6181 aaccaggtca aaatagtttt tcaactgggt cgagatattg aagttataca tcggtgccgc
     6241 aataacgata acgtcgtggg ctttcagctc ggtaatcagc tcatcggaaa gcgccagagc
     6301 ttcctgctga cgcggagtca gcggcgcatc gctcggacgc agtgcaccaa ccagttcacc
     6361 atccaggacc ggaatcggat ttgcagccag gtcacgcacg gtgatttcat cagcggagtg
     6421 cttttcgcgc cattgttcaa caaaataatc ggacaactga ttagactgag agtaccctgc
     6481 caggatgctg gatttaagaa ctaatacctt gctcatggtg tttccttata gatatttgaa
     6541 tgggcaatgc cccgttgctt gttgacactt tattcacaat cctgccacag agatagcgca
     6601 ataaatcgaa gcctatgttc gaatttattg aacaacgcat agaaagctgc gatgtggtac
     6661 tctatatcta tcatttaaaa gaaaattaat caggcagcct actgcccact aacgttatga
     6721 cagaacaaca aaaattgacc tttactgcct tgcagcagcg gctggattcg ttgatgctgc
     6781 gtgacagatt gcgtttttct cgccgtctgc acggtgtgaa gaaggttaaa aatcctgatg
     6841 cacaacaggc cattttccag gagatggcga aagagattga ccatgcggca gggaaagtcc
     6901 tgctgcgtga agcggcacga ccggagatta cttacccgga caatttgccg gttagtcaga
     6961 aaaagcagga cattctcgaa gcgattcgtg accaccaggt ggtgatcgtc gccggggaaa
     7021 cgggttccgg taaaacgact cagctaccga aaatctgtat ggagctgggg cgcgggatta
     7081 aagggctgat cggccatacc cagccgcgtc ggctggcggc gcgaacggtg gcgaaccgta
     7141 ttgcggaaga gctaaaaacg gagccgggcg gttgcatcgg ttacaaagtg cgtttcagcg
     7201 atcacgtcag tgataacacg atggtcaagc tgatgaccga cggtatcctg ctggcggaga
     7261 tccagcaaga ccgcctgctg atgcagtacg acaccatcat tattgacgaa gcgcacgaac
     7321 gcagcctgaa tatcgatttt ctgctcggct atttgaaaga gttgctgccg cggcgtcctg
     7381 acctaaaaat cattatcact tccgcgacta tcgacccgga acgcttttcg cgccacttta
     7441 ataatgcgcc gattatcgaa gtctccggtc ggacctatcc ggtggaagtg cgctatcgcc
     7501 cgattgttga agaagccgat gataccgagc gcgatcagtt gcaggcgatt tttgacgccg
     7561 tagacgaact gagccaggaa agccctggcg acattctgat ctttatgagc ggcgagcgag
     7621 aaattcgcga taccgccgat gcgctgaaca agctgaactt acgccatacc gaaatcttgc
     7681 cgctttatgc acggctttcc aacagcgaac agaaccgcgt gttccagtcg cacagcgggc
     7741 gacgcattgt actggcgacc aacgtcgcgg aaacctcgct gaccgtaccg ggtattaaat
     7801 acgttatcga cccaggtaca gcgcgtatca gccgctacag ctatcgcacc aaagtgcagc
     7861 gtttgccgat tgagccaatt tcgcaggctt cagctaatca gcgtaaaggc cgctgtggtc
     7921 gtgtgtccga agggatctgt attcgtctct attccgaaga cgatttcctt tcgcgcccgg
     7981 agtttaccga tccggagatt ctgcgtacca acctggcctc ggttattttg cagatgaccg
     8041 cactgggctt gggcgatatc gccgcattcc cgtttgtcga agcaccggat aaacgcaata
     8101 tccaggatgg cgtgcgtctg ctcgaagaac tgggcgcgat caccactgat gaacagcaaa
     8161 gtgcttataa actgacgccg ctcggtcgtc agctctcgca gttgcctgtc gacccacgtc
     8221 tggcgcgtat ggtgctggaa gcgcaaaaac atggctgcgt gcgtgaggcg atgattatca
     8281 cgtccgcgct ctccattcag gatccgcgcg agcgtccgat ggataaacag caggcagctg
     8341 acgaaaaaca tcgtcgcttc cacgacaaag agtctgactt cctggcattt gtgaatctgt
     8401 ggaattatct tggcgagcag caaaaggcgc tttcttcaaa cgccttccgt cgcctgtgtc
     8461 gtaccgatta tctcaactat ctgcgcgtgc gcgaatggca ggatatctac acccagttgc
     8521 gccaggtggt gaaagaactt ggcattccgg ttaacagcga accggcggat tatcgcgaaa
     8581 ttcacattgc gttgctgacc ggtttgcttt cgcatatcgg catgaaagat gccgataaac
     8641 aagaatatac cggcgcacgt aacgcgcgtt tctccatctt ccccggttct ggtttattca
     8701 aaaagccgcc gaaatgggta atggtggcgg aactggtaga aaccagccgc ctgtgggggc
     8761 gcattgctgc gcggatcgac ccggaatggg tggagccagt tgctcagcat ttgattaaac
     8821 gcacctacag cgaaccgcac tgggaacggg cgcagggcgc ggtgatggca acggaaaaag
     8881 tcactgttta tggtttgccg attgttgccg cgcgcaaggt caactacagc cagatcgatc
     8941 cggcgttatg tcgtgaactc tttattcgcc acgcgctggt ggaaggtgac tggcagacgc
     9001 gtcacgcatt cttccgcgaa aacctgaaac tacgggcgga agtagaagag ctggaacaca
     9061 aaactcgtcg ccgcgatatt ctggtggatg acgaaacgtt gtttgagttc tacgaccagc
     9121 gcatcagtca cgatgtgatc tctgcacgcc atttcgacag ttggtggaaa aaagtcagcc
     9181 gcgaaacgcc tgatttgctc aactttgaaa agagcatgtt gatcaaagaa ggggcggaaa
     9241 aaatcagcaa gctggattac ccgaacttct ggcatcaggg caatctcaag ctgcgtttga
     9301 gttatcagtt tgagccgggg gcagatgctg acggcgtgac cgtacatatt ccgctgccgc
     9361 tgcttaacca ggttgaggag agcgggtttg agtggcagat ccccggcctg cgccgtgagc
     9421 tgatcatcgc cctgattaaa tcgttgccga agccggtacg ccgtaatttt gtacctgcgc
     9481 caaactatgc cgaagcgttt ttaggccgcg tcacaccgct ggaattgccg ttgctcgaca
     9541 gccttgaacg agagcttcgg cggatgactg gcgtcaccgt tgaccgcgaa gactggcact
     9601 gggatcaggt gcccgatcac ctgaaaatca ccttccgcgt ggtggatgac aaaaacaaga
     9661 agctaaaaga agggcgctca ttacaggatc tgaaagacgc cctgaaaggc aaagtgcagg
     9721 aaacgctgtc tgccgtggcg gatgacggta tcgagcagag cggtttacat atctggagtt
     9781 ttggtcaact gccggaaagc tacgagcaga agcgcggcaa ctacaaagtg aaggcttggc
     9841 cggcgctggt ggatgagcgt gacagcgtag cgatcaaact gtttgataac ccgctggaac
     9901 agaagcaggc aatgtggaac ggtcttcgcc gtttactgct gctaaatatt ccatcgccga
     9961 tcaagtactt gcacgaaaag ctgccaaaca aagccaagct ggggctgtac tttaacccgt
    10021 atggcaaagt gctggagctg atcgatgact gtatctcctg cggtgtggat caactgatcg
    10081 acgccaatgg tggcccggtc tggacggaag aaggctttgc tgcgctgcat gaaaaagtcc
    10141 gtgctgaact gaacgacacg gtggtggata ttgcgaagca ggtcgagcaa atcctcactg
    10201 cggtgttcaa tatcaacaaa cgtctgaaag ggcgggtgga tatgaccatg gcgctggggc
    10261 tttctgacat taaagcgcag atgggcgggc tggtgtatcg cggttttgtc actggtaacg
    10321 gcttcaaacg actgggcgac acgctgcgat atttgcaggc aattgaaaaa cgactggaaa
    10381 aactggcggt cgatccacat cgcgaccgtg ctcagatgct gaaagtcgaa aacgtccagc
    10441 aggcgtggca gcaatggatc aacaaactac cgcctgcacg tcgtgaggat gaagacgtga
    10501 aagaggtccg ctggatgata gaagagctgc gcgttagtta cttcgctcaa caacttggca
    10561 cgccttatcc gatttcggat aagcgtattt tgcaggcgat ggagcagatt agcggttaac
    10621 actgctattt gcctgataaa tctaaaaccc ggtaagcatt tagcgccggg ttttttatta
    10681 attctaaaaa cgataaggga aaaaattcag gagttcagaa aaatacaatt ctctgctgca
    10741 agatgaataa tgtttatcta cagcatttcc ttaaaagata tgtcaggctt gcggagtggc
    10801 agttcaggaa atacgacttc ctcctttcag agtgctccgc ttctcactat tatctcacgc
    10861 agtattctta agggaacgat aaggaggaac catgaatatt accccgtttc cgacgctttc
    10921 gccggcaacc atagatgcca taaatgttat cggacagtgg ctggcgcagg atgatttctc
    10981 cggtgaggtg ccgtatcagg ccgattgcgt gatccttgca ggcaatgcgg ttatgccgac
    11041 tatcgatgcg gcatgtaaga ttgcccgcga tcagcaaatt cctttactga ttagtggtgg
    11101 tatcggtcac tcgacaactt ttttgtatag cgccatcgca cagcatccgc actacaacac
    11161 tatccgcacc actggcagag cagaagctac cattctggcg gatatcgctc atcagttctg
    11221 gcatattccg catgaaaaaa tctggattga agaccagtca acaaactgcg gtgaaaacgc
    11281 acgctttagc atcgcgttat tgaatcaggc tgtagaacga gttcatacgg ctatcgttgt
    11341 tcaggacccc accatgcagc ggcgcacgat ggcgacgttc cgccgtataa ccggggataa
    11401 tcccgatgca ccacgctggt taagttatcc cggattcgtt cctcagttag gaaataacgc
    11461 agagagtgta atctttgtta atccgttgca aggattatgg ccggttgagc gttatctatc
    11521 gctactcact ggcgagctgc cgcgtttacg cgatgatagc gatggctacg gcccccgcgg
    11581 gcgagatttt atcgttcacg ttgattttcc ggcagaagtc attcaggcat ggcaaacgct
    11641 gaaacatgat gcggtgctca tcgaggcgat ggaaagtcgc tcgttacgtt aaaaattgcc
    11701 cgtttgtgaa ccacttgttt gcaaacgggc atgatttctg acttttattt ctgcctttta
    11761 tttctgcctt taattccttt tacacttgtt tttatgaagc ccttcacaga attgtccttt
    11821 cacgatgccg tctctctgat gattgatgtt aattaacaat aaatttacca aaaacaaaca
    11881 tgcaaatcac aggagtcgcc catgtcagta cccgttcaac atcctatgta tatcgatgga
    11941 cagtttgtta cctggcgtgg agacgcatgg attgatgtgg ttaaccctgc tacagaggct
    12001 gtcatttccc gcattcccga tggtcaggcc gaggatgccc gtaaggcaat cgatgcagca
    12061 gaacgtgcac aaccagaatg ggaagcgttg cctgctattg aacgcgccag ttggttgcgc
    12121 aaaatctccg ccgggatccg ggaacgtgcc agtgaaatca gtgcgctgat tgtcgaagaa
    12181 gggggcaaga tccagcagtt ggctgaagtc gaagtggctt ttactgcgga ctatatcgat
    12241 tatatggcgg agtgggcacg gcgttacgag ggcgagatta ttcaaagcga tcgtccagga
    12301 gaaaatatcc ttctgtttaa acgtgcgctt ggcgtgacta ccggcattct gccgtggaac
    12361 tttccgttct tcctcattgc ccgcaaaatg gctcccgctc ttttgaccgg taataccatc
    12421 gtcattaaac ccagtgaatt tacgccaaac aatgcgattg cattcgccaa aatcgttgat
    12481 gaaataggcc ttccgcgcgg cgtgtttaac cttgtactcg ggcgtggtga aaacgttggg
    12541 caagaactgg cgggtaaccc aaaggttgca atggtcagta tgacaggcag cgtctctgca
    12601 ggtgaaaaga ttatggcgac tgcggcgaaa aacatcacca aagtgtgcct ggaattgggg
    12661 ggtaaagcac cggctatcgt aatggacgat gccgatattg aactggcagt caaagccatc
    12721 gttgattcac gcgtcattaa tagtgggcaa gtgtgtaact gtgcagaacg tgtttatgta
    12781 cagaaaagca tttatgatca gttcgtcaat cggctgggtg aagcgatgca ggcggttcaa
    12841 tttggtaacc ctgctgaacg caacgacatt gcgatgggac cgttgattaa cgccgcggcg
    12901 ctggaaaggg ttgagcaaaa agtggcgcgc gcagtagaag aaggggcgag agtggcgttg
    12961 ggtggcaaag cggtagaggg gaaaggatat tattatccgc cgacattgct gctggatgtt
    13021 cgccaggaaa tgtcgattat gcatgaggaa acctttggtc cggttctgcc ggtggtcgca
    13081 tttgacacgc tggaagaggc tatctcaatg gctaatgaca gtgattacgg cctgacctca
    13141 tcaatctata cccaaaatct gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt
    13201 gaaacttaca tcaaccgtga aaacttcgaa gctatgcaag gcttccacgc tggatggcgt
    13261 aaatccggta ttggcggcgc agatggtaaa catggcttgc atgaatatct gcagacccag
    13321 gtggtttatt tacagtcgta gtgagtgaaa gaggcggagg ttttttcctc cgcctgtgcg
    13381 cgtcagagtt tagcgaattt ttcgagggtg cgaataagct gcgtgacgaa gccatattcg
    13441 ttatcgtacc aggcgaccgt tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt
    13501 tgcgtggcat caaacaccga accgaaatgg ctgccaatga tatcggaaga gactatttct
    13561 tcatcggtat aaccaaatga ctcgttattg tttgtcgcct ttttaagcgc gttgttcacc
    13621 tcttcggcag tcactttttt tccgagaatc gacaccagtt cagtgaccga acctgttttc
    13681 accggcacgc gttgcgcatg acctttcagt ttgccgctca gttccgggat caccaggcca
    13741 atggcttttg ccgcccccgt agtgtgggga atgatatttt ctgccgctgc gcgtgaagca
    13801 cgtaaatctt taccacgcgg gccatccacc agtgactggg tgccggtata ggcatgaatg
    13861 gtcgtcatcg tgccgacttc tatcccgaaa ctgtcatgca aggctttggc catcggcgca
    13921 aggcagttag tggtgcatga cgccacggaa acaatggtgt cgttgccatc cagagtgtcg
    13981 tcattgacgt tataaacgat ggttttcatt tcaccggcag gggcggaaat caacaccttc
    14041 ttcgcgccag catcaagatg cgcctgcgat ttctcggcgg aggtataaaa gcctgtacat
    14101 tcgacaatga tttctgcgcc tttcgctttc cacggaatat ttttagcctc tttttcggcg
    14161 taaaccgcga tacttttccc atcaacgata agtgaatctt ccgtataatc aacgctccag
    14221 gggaacggtc cgtagtttga atcatgtttc agcaggtagg cgagaatttt tggggaagtg
    14281 agatcattaa tagcgacaac gtctatgttg cttttgactt caagtaatcg acgcaacacc
    14341 agtcgaccga tacgaccaaa accattaata ccaactttac tcatggtctt ctcctgtcag
    14401 gaacgttcgg atgaaaattg atcctttcca ggcttagacc aggatggcgg gatgggcaat
    14461 ctacattctt gcagtgaaac gtaacgtact gaaaacgggt gaacaatatt taatgaaatt
    14521 ttaagaaaag cccgttatgt taacggcaaa ttatgttaaa gcaggaaatg ttatgggaaa
    14581 taaatactca aggttacaaa tcagcattca ctggctggtc tttttgctgg ttatcgtagc
    14641 gtattgcgca atggagtttc gtggtttctt cccacgtagc gatcggcccc tcatcaacat
    14701 gattcatgtt tcctgtggca tctcaattct cgtactgatg gtggttcgtc ttttgttaag
    14761 gctgaaatac ccaaccccgc cgattatacc taagccaaaa ccgatgatga cgggactggc
    14821 gcatttgggc catttggtga tttatctcct gtttattgcg ctgccggtga ttggtttggt
    14881 gatgatgtat aaccggggca acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc
    14941 agaggccaat ttcgagcggg ttgatagctt aaagtcgtgg catgaaacgc tggcgaatct
    15001 gggatatttt gtcatcgggt tgcacgctgc ggctgcactg gcacaccact atttctggaa
    15061 ggacaacaca cttctacgca tgatgccacg taaacgttcc tgaaggatat ttaaagaaaa
    15121 cgcctgtact aaaaccgacc cgtggtacag gcgaagaata cgggcctaca tcggaagcgc
    15181 ctattatatt tatttgtatg acaaataaaa ccataatcct tgtctatacg tccatctggc
    15241 ttatctttaa tcaattcacc cgatctttga tctcatcaac ggtatcaaaa taaaccagcg
    15301 tataacgttc aaatcgaaca taagattcga taaatcatgg aggttatatg aaaaaactgg
    15361 cacttatcct ggttatggga acgcttgttt ccttttatgc cgatgccggg cgcaaaccct
    15421 gttctggttc gaaagggggg atctcacact gtacggcagg cggcaaattt gtctgtaatg
    15481 atggttctat tagtgcatcg aaaaaaacat gcactaactg aagtgtaaaa ggggggccat
    15541 gagaaaatga atccgctagt cagatgcgcg gggaaaaccc ccgcgcttgc ccttacctgg
    15601 acgtgcaggc catgagcgca gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga
    15661 gcgtttgtcg ggtcaggagt gtgaatgtca gaatcgtaat gcagataatg agcagacaca
    15721 ccaccagagg gttgtgcttc atagcctttc tccttgccgg atggcgggta agaggctaag
    15781 atctgaattg ctaggttcat tcgttggcct cggttgatag aaatatcggt cggggccttc
    15841 gtctttctga ttccaggtta gcctgaaaac agaaagtctc aggcacccgt gagcatccta
    15901 tgtggtttcc ttagggacga aaataatcac ctgacgaaat cgcgtgctgc tttccagaat
    15961 ttttcgtcat tcggtttagc cagtttaatc attcgttact ctcgcaaggc cgtggatttg
    16021 ccgttgtcgc cagcgaagta cggacactcg caagccgcag cgcccaggcg gcgaaagaga
    16081 ttgaaggctt gatcagtgaa tcagtcaggt taattgacct gggatcggat gaggtggcaa
    16141 cggcagggaa aaccatgtgc actattgttg atgccgtcgc gagtgtcaca catatcatgc
    16201 aggaaatcgc caccgcctcg ggtgaacaaa gtcgaggcat aacgcaggtt agccaggcga
    16261 tttctgaaat ggataaggtg acgcaacaga atgcttctct ggtagaagag gcctcaacgg
    16321 cggcggtgtc ccttgaagaa caggcggcac gactaactga ggcggtggac gtattccgtc
    16381 tgaacaaaca ttctgtgtcg gcagaaccac gaggagcggg tgaaccagtt agttttgcta
    16441 cggtgtgaaa atgttaaagg agggatcgac agatcccttc acctttcaga acggcattga
    16501 ttttcgaata gcgttaatca tcaactggca accagaagag aacgtcgcat ctacgcgggt
    16561 cagtattcca atcggttcgc ctgcaccatg tcccggaaca ggcagggcta ccagcgtggc
    16621 atgacgcagg tcgtctttta cagcgccaga agggacaaac cacacgtaat cgtattcaac
    16681 cgtaagttga cgagatagcg aagcagacag cgtttcgata caacccgaag gaattttaca
    16741 tccctggctc tgcactaatg catctgaatg ctggcgtggc gcagtgcctt ctggtgatac
    16801 aacgaccggc cattccagca cccggcttag cgttacgttc tcctgaagta gcgggtgatt
    16861 agggcggaca accagcttca gcgattcaag aaacagtagt tcgtaattaa gcccgttcat
    16921 cagttcagga tctgacatcc gaccaatgcc gatatcgatt tccccggttt ttaaacccgc
    16981 cagaatcata gggttactca ttgtcgcaac ttgcaaggtc gtctcttttt gttgctgatg
    17041 aaactgacct ataaccgaag gtaatatccc tagtgccgca gtaggtagcg caccaaccct
    17101 gacgacatca ttattaagac cttctttacg atgaagcgac tgtccggcag tgttgatggc
    17161 gtcaagaact ctgactgcat gcgttaaaaa ttgttcgccg ggtaaggtaa gttgcgcccc
    17221 ctgacgacca cgctcaaaca agcgagcgcc cgtcagctgc tccagttcat tcaatgtctt
    17281 agagagcgca ggttgactca aattaagggt ttcagccgcg cgccccaaag ttccttgttg
    17341 tgcgacagct acgaatgtat gaaggtggcg caaacggatg cgctgactaa acagactatt
    17401 tttttccata agcgatgtta aaaacgaagc ggtgtcgctg acaagtgaag ttgttttatt
    17461 atgataactt gattgcaaaa tattattaac aattaaagca actatgttac agcaaaatgg
    17521 ataatattga tgttttcgcg gcgagatcac agtttgtaaa ttcttccagc cggagtgaat
    17581 gcggttacct acactccaga ttactgacca ctggaggcag acactatggc gaacagcatc
    17641 acggcggatg agattcggga acagttttcg caggcaatgt cagccatgta ccagcaagaa
    17701 gttccgcagt acggcacgct gctggaactg gtagctgatg taaatctggc ggtgctggaa
    17761 aacaatcctc aactgcacga aaaaatggta aatgcagacg aactggcgcg actgaatgtt
    17821 gaacgtcatg gggcgattcg cgttgggact gcacaagagc ttgctactct tcggcggatg
    17881 tttgccatta tggggatgta cccggtgagc tattacgatc tctcgcaggc aggggtgccg
    17941 gtacattcga cagcatttcg gcccattgat gctgcttctc tggcgcgtaa tcccttccgc
    18001 gtttttacct cattactccg ccttgagctt atcgagaacg aattattacg ccagaaagcg
    18061 gcggagattc tgcgtcagcg cgatatcttc accccacgtt gtcgacaact gttacaggaa
    18121 tatgagcagc ggggcggttt taacgaaaca caggcacagg agtttgtgca ggaagccctg
    18181 gaaacgtttc gctggcacca gtcagcaacg gtagatgaaa aaacctatcg cgccttgcac
    18241 aaagaacatc ggttgattgc tgatgtggtc tgttttcctg gatgccatat caaccacctg
    18301 acgccacgca cgctggatat tgaccgggtg cagtcgatga tgcctgaatg cggaattgaa
    18361 cccaaaattc tgatcgaagg gccgccgcgc cgcgaggtgc cgattttact acgccagacc
    18421 agctttaaag cactggaaga gacggtgttg tttgcggggc agaaacaggg cacacatacc
    18481 gcgcgctttg gtgaaattga gcagcgtggt gtggcattaa cgccgaaagg gcgacaactg
    18541 tatgatgatc ttctgcggaa cgctggaacc gggcaggata atctcactca ccaaatgcat
    18601 ttacaggaaa ccttccgcac ttttcctgac agtgagtttt taatgcgtca gcaagggctg
    18661 ggatggttcc ggtaccgtct gacgccttcg ggtgaggcgc atcgtcaggc gattcatccc
    18721 ggagacgatc cacagccctt aatagaacgt ggttgggtag tggcgcaacc cattacctat
    18781 gaagatttct tgcccgttag cgcggcgggg atcttccagt caaatctggg taatgaaacg
    18841 caggcatgca atcacggtga tgccagtcgc gaagcatttg agcaggcgtt ggggtgtccg
    18901 gttttggatg agttccagct ttatcaggaa gcggaagaac gcagtaaacg tcgctgtggt
    18961 ttgctttaaa ttctgaccat ccgcctttgc aaaaatttgc ctgattttac aaacgaatca
    19021 ggctcatccc atcgacataa aaaaaatgcc aatttatgca tattctctga tttcaacaat
    19081 tggattatta ataaatattg tctagagtga gcggtcataa ataagcactt tcttgccgct
    19141 gaaaatgacc agcgcgggac cattcacaac accagaagga ctcactttca ggtatggatc
    19201 gtagacgatt tattaaaggt tcaatggcta tggccgccgt gtgcggtacc agcggcattg
    19261 cttctctttt ttctcaggcg gcattcgcgg ccgattctga tattgccgac gggcaaaccc
    19321 agcgttttga cttctccatt ctacagtcaa tggcgcacga cttagcgcaa acagcatggc
    19381 gtggtgcacc acgtccgtta cctgatactc tggcgacaat gacgccgcag gcttataaca
    19441 gtattcaata cgacgccgaa aaatcgctct ggcataacgt tgagaaccgt caactggacg
    19501 ctcagttttt ccatatggga atgggattcc gtcgccgcgt tcgtatgttt tctgtagatc
    19561 cagcaacaca tctggcgcgt gaaattcact ttcgcccgga gttgttcaaa tacaacgatg
    19621 cgggtgtgga taccaaacaa ttagaagggc aaagcgatct cggttttgcc ggttttcgcg
    19681 tgtttaaagc ccccgaactg gcgcgccgtg atgtagtatc attcctcggc gcgagttatt
    19741 tccgcgccgt tgatgacaca tatcaatacg gtttgtcggc ccgcggcctg gcgatcgaca
    19801 cttacaccga cagcaaagaa gagttccccg actttaccgc cttctggttt gatacggtaa
    19861 aaccaggggc aaccaccttt accgtttatg cgttgctcga tagcgccagc attactggtg
    19921 cctataagtt cactatccat tgtgagaaaa atcaggtgat tatggatgtg gaaaatcacc
    19981 tgtatgcgcg caaagacatt aaacagctgg gcattgcacc gatgaccagt atgttcagct
    20041 gcggtactaa tgaacgtcgg atgtgcgaca ccattcatcc gcaaatccat gactctgatc
    20101 gtctgtccat gtggcggggc aacggcgagt ggatttgccg tccgctgaac aatccgcaaa
    20161 aattgcagtt caatgcttac accgacaaca acccgaaagg gtttggttta ttgcaactgg
    20221 atcgtgattt ctcccattat caggacatta tgggctggta taacaaacgc ccaagtctgt
    20281 gggtggaacc gcgtaacaag tggggtaagg gcaccatcgg cctgatggaa atcccaacta
    20341 cgggggaaac gctggataac attgtttgct tctggcagcc agaaaaagct gtaaaagcgg
    20401 gtgatgagtt tgcattccag tatcgtctgt actggagtgc gcaaccgcct gttcattgcc
    20461 cattagcgcg cgttatggcg acgcgtaccg gcatgggcgg tttcccggaa ggttgggcgc
    20521 caggtgaaca ctatcccgaa aaatgggcgc gacgttttgc cgtcgatttc gttggtggcg
    20581 atctgaaagc tgccgcgcca aaaggcattg agccggtgat tacgctttcc agtggggaag
    20641 cgaagcaaat cgaaattctc tatattgaac ccattgatgg ttatcgtatt cagtttgact
    20701 ggtatccgac ttcggactcc actgatccgg tcgatatgcg gatgtatctg cgttgtcagg
    20761 gcgacgctat cagtgaaaca tggttatatc agtatttccc gccagcgccg gataaacgtc
    20821 agtatgttga tgaccgcgtg atgagttaat cgttttttct tcggcatctt cttcgggagg
    20881 tgccgtctgg ttaaacacga tcccgctcgc attttttcct aagttaaatg agtaatctgc
    20941 tggtgtgtat ttcagataca ccttgtcagc cactaacagg gagtgcgtat gtttccagaa
    21001 taccgagatt taatatcccg tctgaaaaac gaaaatcctc gctttatgtc cttgttcgat
    21061 aaacacaata aacttgatca tgaaattgcc agaaaggaag gttccgacgg tcgagggtac
    21121 aatgcggaag tggtccgcat gaaaaaacaa aagctacagt taaaagatga gatgctcaaa
    21181 atcctgcagc aggtgagcgt caaagaggtg taaactttcc taagccgcca gccaggcggc
    21241 ttttttaaca gctgcatgga ttgactggag ataagatgac tgaaacgata aaagtaagcg
    21301 aatcacttga attacatgct gttgcggaaa gtcatgtcac acttctttat cagttaatct
    21361 gtaaaaataa aacctggtta cagcagtcgc taaactggcc gcagtttgtt caaagtgaag
    21421 aggacacgcg aaaaacggtg cagggtaatg tgatgttgca tcaacgcggc tatgccaaaa
    21481 tgttcatgat tttcaaagaa gatgaactta tcggcgttat ctcttttaat cgtattgaac
    21541 cactgaataa aaccgctgaa atcggctact ggctggacga acctcatcag gggcagggga
    21601 tcatttctca ggcgctgcag gcattgattc atcattacgc tcagtctggt gaacttagac
    21661 gcttcgtgat caaatgtcgg gtggacaatc cgcaaagcaa ccaggtagcg ttgcgcaatg
    21721 gttttatcct tgaaggttgc ctgaaacagg ctgagtttct gaatgatgcc tatgatgatg
    21781 tgaacttata cgcgcgtatt atcgattcac aataaccctg ccagcagcgt tcgcgtaatg
    21841 cgctcctcgc cgttaatgac ttttggacca cggacatgaa tcgtgtcgcc atcaaaggct
    21901 tcaacgacgg cgtggtcagt taactccaca tggttttcaa ttatcaccgc acctgtaata
    21961 cggctctcac cttgtattac aacgtgctcg tcgagcagaa tcggtccgcc acgtaccacg
    22021 gcatttccgc caatcaagac atgatgtttt aacacacaat taccttccac aatggcatat
    22081 tccgccacct gtgaactgta atgaatcgtc ggaatggcat cttcttctat gccagctttc
    22141 acctgcgcgt gaccgtaaac tttagcgcaa tcgcatagcc agacgttgtt ctcttcatta
    22201 ccttcaatac tggcaaaatc aaaaacttcg gcgcgatgtt caataaaagc ataccggacg
    22261 acggcatcgc cataaatttg tgcctggtgg acaatacgcg aggcgctaac ctttgcgcga
    22321 tcataaattt gtagcaggag ttgatggtcg ggcgttaagc cttgtgcggc gacgatcata
    22381 gaatgttgat caattaaggc gtgaccaaat attctacatt gaccgcaaac taatgagtca
    22441 ctaatggtga cactgtcact tatatatgca ccctggctga tttcactatt atcgatccag
    22501 acattatccg ttgcgtaaac ctctccccac agcacgctgg tgccggtgat acgcgtattc
    22561 ccggaaatca ccgttccgcc gaacgcaata gcattctggt cgtaaatcca gcaatttcct
    22621 tcctgtgcta acaccgtttc gcgatcgatc cagccgccag cgcttcctgc tatcacatcg
    22681 ttaaaatcgc tcatggcgat aatttgccgt aataacacac tttttttagt gccatcttcc
    22741 tgataactaa aggctcgctg ttcttcactt agacgatatt tacgcatagc gttttcccac
    22801 aggtgactta ctataaccgt agcaaattct gcggctctgg ctatgctcga gaaaattcca
    22861 taaaatgcat ttcaaatata ctttataaat taaacaaaat gagtaagaag atgcagagcg
    22921 ataaagtgct caatttgccg gcaggctact ttggtattgt gttggggacg atagggatgg
    22981 gatttgcctg gcgctatgcc agccaggttt ggcaggtcag ccactggttg ggggatgggc
    23041 tggtgattct ggcgatgatc atctggggat tattgactgg cgcatttatt acccgactca
    23101 tacgctttcc gcatagcgtg ctggcagaag tgcgccatcc agtgctgagc agttttgtga
    23161 gtttgtttcc ggcaacgacg atgctggtgg cgattggttt tgttccgtgg tttcgcccac
    23221 tggcggtgtg cctgttcagt tttggtgtcg tggttcagtt ggcttatgcc gcctggcaaa
    23281 ctgcgggatt atggcgtgga tctcaccctg aagaagctac cacgcctgga ctgtatctgc
    23341 cgacagttgc caacaacttt atcagcgcaa tggcctgtgg tgcgttgggc tacaccgacg
    23401 ccggtctggt gtttttaggc gcaggcgttt tctcatggct aagcctggaa ccggtgatct
    23461 tgcagcgtct gcgcagttcg ggagaattac ccacggcact gaggacatca ctcggcattc
    23521 agctcgctcc tgcgctggtg gcctgtagtg cctggctgag cgtcaacggc ggcgagggtg
    23581 acacgctggc gaaaatgctt ttcggttatg gactgctgca actgctgttt atgctatgtc
    23641 tgatgccatg gtatctctcc cagccattta atgcttcatt ctggagtttc tcgttcggcg
    23701 tatctgcact ggcaaccacc ggtttgcatc tggggagtgg cagcgataat ggatttttcc
    23761 atacgctggc ggtgccgctg tttatcttta ccaattttat tattgcaata ctgctcatcc
    23821 gtacttttgc gcttctgatg cagggaaaat tgttagtcag aaccgagcgc gccgttttaa
    23881 tgaaagcaga ggacaaagaa tgatcattcg tgatgaaaac tattttactg ataaatatga
    23941 attaacccgc acacactctg aagtgctgga agcggtgaaa gtggttaaac cgggtaaaac
    24001 gctggatctg ggctgtggca atggtcgtaa cagtctttac ctggcagcca atggttatga
    24061 tgttgacgca tgggataaaa atgccatgag tatcgccaac gtcgagcgca ttaagtccat
    24121 tgaaaatctg gataatttac acacccgggt cgttgatctg aataacctca catttgatgg
    24181 acagtacgat tttattcttt cgactgtggt gctgatgttc cttgaggcta aaaccatccc
    24241 agggctgatt gccaatatgc aacgttgcgc taaacctggc ggttacaacc tgattgtggc
    24301 ggcgatggat accgctgatt atccatgtac cgtcggcttc ccgtttgcct tcaaagaggg
    24361 ggaattacgt cgatattacg aaggctggga gatggtgaaa tacaatgaag acgtcggcga
    24421 gctgcaccgc accgacgcca acggtaatcg tattaaactg cgtttcgcca cgatgctggc
    24481 acgtaaaaaa tgatccggta agaacaaagc gcgtgaaaat tccccacgct gagatggttt
    24541 actgttcttc ttttcggtaa gcatattttt tatcgaaggg atgtgaaatt aatcacagta
    24601 gtcgaagttt tttagccgct taacgtactg aaatttaact actgatgatt gacttagccc
    24661 ctttttcggc attgactatg tcgtctgaaa aggggctaaa aaatttattt tcaccaacac
    24721 tttttttgcc acaacacgaa gcggcgcttt ttgctataac ttaaaaagta atataatcat
    24781 ctcaggaaac tattcatgcg taccacatca tttgcgaaag ttgcagcttt atgcggcttg
    24841 ttggctctgt ctggttgtgc ctctaaaatc acccagccag ataaatattc tggtttttta
    24901 aacaattact ctgatttaaa agaaacaacc tcggcaacag gtaaacctgt tttacgttgg
    24961 gtagacccga gttttgatca aagcaaatat gacagcatcg tctggaaccc aatcacttat
    25021 tatccggtac cgaaaccttc tacccaggta gggcagaaag ttctggataa aattttgaac
    25081 tataccaaca ccgaaatgaa agaagcgata gcgcagcgta aaccactggt taccaccgct
    25141 gggccgcgta gtctgatttt ccgtggggcc attaccggtg tagataccag caaagaaggg
    25201 ctgcaattct atgaagtggt tcctgttgca ttagtggttg cggggacgca aatggctaca
    25261 ggccaccgta ccatggatac tcgcttgtat tttgaaggtg agctgattga tgcagcgact
    25321 aataaaccgg ttatcaaagt cgttcgtcag ggcgaaggta aagacctgaa taacgaaagt
    25381 acgccaatgg ctttcgaaaa tattaaacaa gttattgatg acatggcgac cgatgccacc
    25441 atgtttgacg ttaacaaaaa gtagttccag acgcgccatc gttagatggc gctttttatt
    25501 atcggtttct gttagcgatt aaattcaaca ccacgtaaca aacgcctccg gcaattaatc
    25561 cccaaaacgc agaaccaatc ccgaacagcg tcaatccact tgccgttacc agaaatgcca
    25621 ccaccgccgc gtcgcgctca cgctcattat gcagcgcctg atacaaactg ccgccaatgg
    25681 tactcaacag cgcaagacct gccagcatct ggatccaact tacgggcagg gcagccatca
    25741 tcccggtaat ggcactacca aacagacctg cgatcaaata aaaaatgccg gcaacggcag
    25801 cggccagcca acgttgatct ttatccggat gcgcttccgg gctttggcaa atagccgcgg
    25861 tgattgccgc aataccgacg gaataaacgc cgaaagggga aaaaaacagt gccagcaatc
    25921 cagtaaacac aatcaacggc gaaacaggag ccgaataccc ggctgctttc attgctgcga
    25981 tacccggtgc gttttgcgat gccatcgtca ccagaaaaag ggggagtgca acgctcagac
    26041 tgtgagcaaa cgaaaaatca ggggtaatat aagtggggag aacgggttta aagacaatat
    26101 cagttgtaac aacgtcacct tgcgcggtaa cgatgacgat cccaataatc atcgcggcaa
    26161 ttaccgcata gcgcggcgca acggccttgg ctgccagcca tgccagcaac atactgccac
    26221 acaacgtaaa ttgaccatcc agactggcaa acgcctgtaa accaaaacgt aataaaatcc
    26281 cggcaagcat tgccgccgca agcgagtgcg gaataatgcg catcagacga gcaaagagtc
    26341 ccgttatgcc gcagaggact attaacgcgt tggtgacaat aaaaacgccg atggcttcgt
    26401 taagtgttag ttcctgcaat ccggtgacca acaaagccgc gccaggcgtt gaccatgcgg
    26461 tgagaacagg tacgcgatac cataatgtca gagtcagcgt actgacgccc attgccagcc
    26521 ccagcgccgt catccagcca gagatttgtg cagtggtggc tccggcgaca atcgctgctt
    26581 gccagattat tgccgctgaa ctggcgtagc caattaatac cgccagaaac cccgccagta
    26641 gcgtgggtgg aggaatagaa aacagacgca tagtcacctc gtgcgttata acgtacaggt
    26701 accgtagcat ttgtccgtta taacgcacaa gtgataaact tccgttttgc cggaggagtc
    26761 gcatggaaaa tctcgctcgt tttttatcca ccacacttaa acaactacgt cagcagcgcg
    26821 gctggagtct ttcgcgactg gcagaagcga cgggcgtttc taaagcgatg ttagggcaga
    26881 ttgagcgtaa tgaatccagc ccgacggtcg cgacattatg gaaaattgcc accgggttga
    26941 acgtgccatt ttcaacattt atttctccgc cgcagtccgc tacgccttca gtttatgatc
    27001 cacaacggca ggcgatggtg atcacttcgc tgtttcctta tgatccacaa ctctgctttg
    27061 aacacttctc gattcagatg gcacccggtg caataagcga atcgatgccc catgaaaagg
    27121 gggtaatcga acatgtcgtc gtcattgatg gacaacttga tctgtgtgtt gatggtgaat
    27181 ggcagagcct taattgtggg gaaggcgttc gatttgctgc agacgtcacg catgtctatc
    27241 gtaatggtgg ggagcaaacc gtacattttc attccctcat ccattacccg cgcagttaag
    27301 cgggaaaact atttcgcaac gtcgcgcttg tggctaaaat agccgccatt tttcagctac
    27361 tggataagaa agtgaccgta tcttctcatc gtcttgaact gttaagcccg gcacgcgatg
    27421 ccgccattgc ccgcgaagct attttgcacg gtgccgatgc tgtttatatc ggcggccctg
    27481 gttttggtgc ccgtcataat gccagtaata gcctgaaaga tattgccgag ttggtgccgt
    27541 ttgcccatcg ttatggtgca aaaattttcg tcacgcttaa caccattttg catgatgatg
    27601 agctggaacc cgcgcaacgg ctgattactg acctctacca gaccggtgtc gatgcgctga
    27661 ttgttcagga tatggggatt ctggaacttg atattccgcc gattgaactg cacgccagta
    27721 cgcagtgcga tattcgtaca gtagaaaaag cgaagttcct ctctgatgtt ggcttcacgc
    27781 agattgtgct ggcgcgagaa ctgaatcttg agcagatccg cgcgattcac caggccacga
    27841 acgcgaccat tgaattcttt attcatggcg cattgtgcgt ggcctattcg ggtcagtgct
    27901 acatttctca tgcgcaaaca gggcgtagcg ccaaccgtgg cgattgctcg caggcgtgcc
    27961 gtttgccata cacattgaaa gacgatcagg ggcgggtggt ttcctatgaa aaacatctgc
    28021 tgtcgatgaa agataacgat cagactgcca acctcggcgc gctgattgat gctggcgtac
    28081 gctccttcaa gattgaaggg cgttacaaag atatgagcta cgtgaagaat atcaccgccc
    28141 attatcgcca gatgctggat gccattattg aagaacgtgg cgatctggcg cgcgcttcat
    28201 caggtcgtac tgaacatttc tttgttccat cgacggaaaa gactttccac cgtggtagca
    28261 cagattattt tgtgaatgcc cgtaaaggcg atattggcgc gttcgattcg ccgaaattta
    28321 tcggcctgcc ggtgggcgaa gtattgaaag tagcgaaaga tcatattgat gttgccgtta
    28381 ccgagccact ggcaaatggc gatggcctga acgtgatgat taaacgtgaa gtcgtcggtt
    28441 ttcgtgccaa tacggtagag aaaaccggag aaaatcagta ccgcgtctgg ccaaatgaaa
    28501 tgccagcaga tttgcacaaa attcgcccgc atcacccact aaaccgtaat cttgatcata
    28561 actggcacca ggcactgaca aaaacctcca gcgaacgtcg ggtggcggta gacattgaac
    28621 tgggcggctg gcaggaacaa ctgattctga ccctgaccag tgaagagggc gtcagcgtca
    28681 cgcatacgct ggacgggcag ttcgacgaag ccaataacgc cgaaaaagca atgaacaatc
    28741 tgaaggatgg tctgacaaaa ctggggcaaa ccatctatta cgcccgcaat gtgcaaatta
    28801 atttgccggg ggcgctgttt gtaccaaaca gtctgttaaa ccagttccgc cgtgaagccg
    28861 ccgatatgct tgatgctgcg cgtcttgcca gttaccagcg cggcagccgt aaaccggttg
    28921 ctgatcctgc gccggtttat ccgcaaacgc atctgagttt cctcgcgaac gtatacaacc
    28981 agaaagcgcg tgaattttat catcgctatg gtgtgcaact gattgacgcg gcgtatgagg
    29041 cacatgaaga gaagggcgaa gtcccggtga tgatcaccaa gcattgtctg cgctttgcct
    29101 tcaatctgtg cccgaaacag gcgaaaggca atatcaaaag ctggaaggcg acgccaatgc
    29161 aactggttaa cggcgatgaa gtattaacgc taaagtttga ttgccgccca tgcgagatgc
    29221 acgtcattgg caaaatcaaa aatcacatac tgaaaatgcc gttaccggga agcgtagtgg
    29281 catccgtaag tccggatgat ctgctgaaaa cattgccgaa gcgaaaaggg taaaacgcca
    29341 gttttcgggt tactcaccac tcatcaaatc tgcatgatat tgcctgccgg gtaaggcgtc
    29401 atgccgcatc cggcaacaaa tgactcaacg ctgtcccgct tctggtttgc gcgatttttg
    29461 ccagtaaaaa tgctcgcgca aaccttccgc cgactcttcc gccacagcac gcaattcatc
    29521 gctgtccgct tcatgacgca gctgatgatc cacattcttt acccacataa attcatgtcc
    29581 tttgtgccct gccatgagtt gtcctgagaa cagtgcacac gttaataaga caaccgataa
    29641 cgccttcgaa aacatcccgc cacctttttc taaccttttg ccgctatgat gccgatcgtt
    29701 tcttgaggtt attattcagt tttgcaaatt agagcaaaga aattctggaa tcttccttcc
    29761 tgattttgca ttgaattctg ccgttgcggc gatttagtgc tattttcgag cacattacac
    29821 acggaggtaa acttcgccat taaatagtca cgatcacttt attgaagtgc ctttgagcgt
    29881 cgcctctaat gtattgctgt taaatgcttt tttacagtca gaaatcactc agcaagagtt
    29941 agccaggcga attggcaaac ataaacagga gattactcgc ctatttaatt tgcaccatgc
    30001 gacaaaaatc gacgccgtcc agctcgcggc taaggcgctt ggcaaagagt tatcgctggt
    30061 gatggtttaa ttacagttaa cgaaaagttg tcatttttaa caactgatat agactgccga
    30121 atcatctgca cataattacg attcgataat gaaaaaatac cagcagcttg cagaacaatt
    30181 acgcgagcag attgcgtcgg gtatctggca acccggcgat cgtttgcctt cgttgcgtga
    30241 ccaggtggcg ctttctggca tgagctttat gactgtcagc catgcctatc agttgctcga
    30301 aagtcaggga tatattatcg cgcgaccgca atcgggttat tacgttgcgc cacaggcaat
    30361 aaaaatgccg aaagcgacag tcattccagt cacacgagat gaagcagtcg atatcaacac
    30421 ctatattttt gatatgttgc aggccagtcg cgatccgtcg gtagttccgt ttgcctcggc
    30481 ctttcccgac ccgcgacttt tccccctcca acaactaaac cgctcgctgg cgcaggtaag
    30541 caaaaccgcc acggcgatga gcgtgattga aaacttaccg ccaggaaacg cagaactgcg
    30601 tcaggctatt gctcgtcgct atgccttaca gggcatcacc atttctcctg atgaaattgt
    30661 cattaccgcc ggggcgttag aggcattaaa cctcagtttg caagcggtaa ctgaaccggg
    30721 cgattgggtg atagtagaga atccttgttt ctacggcgcg ttgcaggcgc tggagcggtt
    30781 acggctgaag gcgttatcgg tagcgacgga tattaaagaa gggatcgatc ttcaggcgct
    30841 ggaactggcg ttgcaggagt atccggtgaa agcgtgctgg ctgatgacta atagccagaa
    30901 cccacttgga tttaccttaa cgccgcagaa aaaagtgcgg ctggtagcgt tgctcaatca
    30961 gtacaacgta acgctgattg aagatgacgt ttatagcgaa ctttattttg gacgggaaaa
    31021 accgctgcct gcgaaagcgt gggatcgcca cgatggtgtt ttgcattgct cttcgttttc
    31081 gaaatgtctg gtgcctggtt ttcgtattgg ttgggtcgcc gccggaaaac atgcacgtaa
    31141 aattcaacgc ttgcagttga tgagtacgct ttccaccagc tcaccgatgc agcttgcgct
    31201 ggtggattac ctttctactc gccgatacga tgctcatctt cgtcgcctgc gtcgccagct
    31261 tgcggaacgt aaacaacgcg cctggcaggc tttgctgcgt tatctgcctg cggaagtcaa
    31321 aattcatcac aatgacagcg gttattttct ctggctggag ctccccgagc cgttagatgc
    31381 aggagaatta agcctggtgg cactgacgca tcatatcagt attgcgccgg gtaatatgtt
    31441 ttctaccggt gaaaactggt cacgtttttt tcgttttaat accgcgtggc agtggggaga
    31501 acgtgaagaa caggcggtaa aacaattagg caaacttatt caagaacggc tgtaatagcg
    31561 tttaatttaa ttcctctcag gttgggtaat atgaatttcg aatagcagtc atatatccta
    31621 actccttgtc tatactccag aagataacct tacagacggc ataatgcgcg gtagctcaca
    31681 acctgattaa attttctcag ggccgaaggt gtgcctgcaa gccgccgtct atggttaaac
    31741 aaggagatat ttttacggca cggcggctga acaattaatt acgacaggag taagacctta
    31801 tgagcaagac atttgcccgc agcagcctgt gtgcgctcag catgacaata atgaccgctc
    31861 acgccgccga accgcctacc aatttagaga atccggaagg gcgactggat attatcgcct
    31921 ggccgggata catcgaacgc ggacaaactg ataaacagta cgactgggta acgcaattcg
    31981 aaaaagagac aggctgcgcg gtgaatgtta aaaccgccgc gacttccgat gaaatggtca
    32041 gtctgatggc caaagggggt tacgatctgg ttacggcatc cggcgatgcc tcgctgcgtt
    32101 tgattatggg caaacgcgtg cagccaatta ataccgcctt gattcccaac tggaaaacgc
    32161 tcgatccgcg cgtggttaaa ggcgactggt ttaacgttag tggcaaagtt tatggcacac
    32221 cttaccaatg gggtccgaac ctgttgatgt acaacactaa aaccttcccg acgccgccgg
    32281 atagctggca agtggttttt gttgagcaga atctgccgga cggcaagagc aataaaggcc
    32341 gcgttcaggc ttatgatggc cctatctaca ttgcggacgc tgcgttgttc gttaaagcca
    32401 ctcagccgca gttgggcatc agtgatccgt atcaactcac cgaagaacag taccaggcgg
    32461 tgctgaaagt gctgcgcgat caacatagtt tgatccatcg ctactggcat gacactaccg
    32521 tgcaaatgag cgatttcaaa aacgagggtg tggttgcttc cagtgcatgg ccctatcagg
    32581 ccaacgccct gaaagccgaa ggccagcctg tcgctaccgt tttcccgaag gagggcgtta
    32641 ccggttgggc tgacaccacc atgctacata gcgaagcgaa acatccggtt tgcgcctaca
    32701 aatggatgaa ctggtcatta accccaaaag tgcagggcga tgtggcggcc tggtttggct
    32761 cgttaccagt agtgccgcaa gggtgtaaag ccagtccgtt attaggcgag aaaggttgtg
    32821 aaacaaacgg ttttaactat ttcgataaaa tcgccttctg gaaaacgcct gtagcagaag
    32881 ggggcaagtt tgttccctac agtcgctgga cgcaggatta cattgccatt atgggcggtc
    32941 gctaacttcg ctggggtgtt ttatgacgta cgcagtggag tttgacaacg tctcacggtt
    33001 gtacggtgac gtgcgcgcag tagatggcgt cagtattgcg ataaaagatg gtgagttctt
    33061 ctctatgctg gggccgtccg gctccggcaa aaccacttgc ctgcgcctga ttgctggatt
    33121 cgaacagctt tccggcgggg ctatctctat ttttggtacg cccgccagca atctgccacc
    33181 gtgggagcgg gacgtgaata ctgtcttcca ggactacgcg ctatttccgc atatgtcgat
    33241 tcttgacaac gtagcctacg ggctgatggt caaaggcgtg aataaaaagc agcggcatgc
    33301 aatggcgcaa gaggcgcttg agaaagtggc gttggggttt gtacagcaac gtaaaccgtc
    33361 acaactttct ggtggtcagc gccagcgggt tgctatcgcc agagcactgg tgaacgaacc
    33421 gcgtgtgttg ttgctggatg aaccgctcgg cgcgctggat ctcaaattgc gagagcaaat
    33481 gcagcttgag ctgaaaaagc tgcaacagtc tctcggtatc acttttatct tcgttaccca
    33541 cgatcagggc gaagcgttat cgatgtccga tcgggtggcg gttttcaata atggacgcat
    33601 tgaacaggtc gattctccgc gcgatctcta tatgcgcccg cgcacgccgt ttgttgcggg
    33661 attcgtcggg acatccaacg tttttgatcc gctaatggcc gagaaacttt gtggcatgac
    33721 gggaagcttc gcactacgac cggaacatat ccgcctcaac acccatggcg aaatgcaggc
    33781 taatggcacg atccaggcgg tgcaatatca gggcgcagcg acccgttttg aactgaaatt
    33841 gaacggcggt gaaaaactgc tggtgagtca ggccaatatg acaggcgaag agctacctgc
    33901 cacgctcacg cccggacaac aggtgatggt ttcctggtca cgtgatgtga tggtggcgct
    33961 ggttgaggag aggtgaatgg cgatgaacgt attgcaatca ccttcacgtc caggtctggg
    34021 taaggtgtcc ggtttcttct ggcgtaatcc ggggctgggg ctgtttttgc tgctgcttgg
    34081 cccgctaatg tggtttggca ttgtctattt cggctcgctg ctgacgctgt tatggcaggg
    34141 cttttatacc tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc tggcgaatat
    34201 ccgcgcgctg tttaatccgg cgaattacga catcattgtc cgcacgctga ccatggctgt
    34261 ggcagtcact atcgccagcg ccattctggc ttttccaatg gcgtggtata tggcgcgcta
    34321 taccagcggg aaaatgaaag cgttttttta tatcgcggta atgttgccga tgtgggcgag
    34381 ctacattgtt aaagcctatg cctggacgtt attgctggca aaagatggcg tggcgcagtg
    34441 gtttttacaa catctggggc tggaaccact gctgactgcg ttcctcacgc taccggcggt
    34501 gggaggtaat acgctgtcaa cttccgggct ggggcgcttt ctggtgtttc tctatatctg
    34561 gttgccgttc atgatcctcc cggtgcaggc ggcgcttgag cgtttgccgc cgtcattgtt
    34621 gcaggcgtcg gctgatcttg gcgcacgtcc acgacaaacc tttcgctatg tggtgctgcc
    34681 gctggcaatc ccgggtattg ccgctggctc tatctttacc ttctcactca cgctgggcga
    34741 ttttattgtc ccgcagctgg ttggccctcc aggatatttt atcggcaata tggtttattc
    34801 ccagcagggg gcgattggca atatgccgat ggcggcggca ttcaccctgg tgccgattgt
    34861 tctcatcgca ctttacctgg cgttcgtgaa acgtctggga gcgttcgatg cactctgaac
    34921 gcgcaccgtt tttcctcaaa ctggcggcct ggggcggcgt ggttttccta cattttccca
    34981 tcctgattat cgctgcctat gcgtttaaca ctgaagatgc ggcgtttagt tttccaccgc
    35041 aaggcctgac gctgcgctgg tttagcgtgg cagcacagcg tagtgatatc cttgatgccg
    35101 tgacactgtc acttaaagtg gcggcgctgg cgacattaat cgcgctggtg ttagggacgc
    35161 tggcagctgc cgcgctgtgg cgacgagact ttttcggcaa aaacgccatt tcgctgttac
    35221 tgttgctgcc cattgcgctg ccgggcattg tcactggtct ggcgctatta accgccttta
    35281 aaaccatcaa tctggagcca ggatttttca ccatcgtggt cggtcatgcg actttttgcg
    35341 tagtggtggt gtttaacaat gtcatcgccc gttttcgccg cacctcgtgg agtctggttg
    35401 aggcgtcaat ggatcttggg gccaatggct ggcaaacctt ccgctacgta gtgttgccga
    35461 atctcagttc ggcgttactg gctggaggaa tgctggcgtt tgccttgtcg ttcgatgaaa
    35521 tcatcgtgac gacttttacg gcaggtcatg aacgaacgtt accgttgtgg ttgctcaatc
    35581 agcttgggcg accgcgtgat gtaccggtaa ccaacgtggt ggcactgctg gttatgttgg
    35641 taacaacctt gccgatcctg ggggcctggt ggctaacccg cgaaggcgac aatggtcaat
    35701 aaccactgat acaggaatat gctatgcaac ataagttact gattaacgga gaactggtta
    35761 gcggcgaagg ggaaaaacag cctgtctata atccggcaac gggggacgtt ttactggaga
    35821 ttgccgaggc atccgcagag caggtcaatg ctgctgtgcg cgcggcagat gcagcatttg
    35881 ccgaatgggg gcaaaccacg ccaaaagcgc gtgcggaatg cctgctgaaa ctggctgatg
    35941 ttatcgaaga aaatggtcag gtttttgccg aactggagtc ccgtaattgt ggcaaaccgc
    36001 tgcatagtgc gttcaatgat gaaattccgg cgattgtcga tgtctttcgc tttttcgccg
    36061 gtgcggcgcg ctgcctgaat ggtctggcgg caggtgaata tcttgaaggt catacttcga
    36121 tgatccgtcg cgatccgttg ggggtcgtgg cttctatcgc accgtggaat tatccgctga
    36181 tgatggctgc gtggaaactg gctccggcgc tggcggcagg gaactgcgta gtgcttaaac
    36241 catcagaaat taccccgctg accgcgttga agttggcaga gctggcgaaa gatatcttcc
    36301 cggctggcgt gattaacgtg ctgtttggca gaggcaaaac ggtgggcgat ccgctgaccg
    36361 gacatcccaa agtgcggatg gtgtcgctga cgggttctat cgccactggg gaacacatca
    36421 tcagccatac cgcgccgtcc attaagcgta ctcatatgga acttggtggc aaagcgccag
    36481 tgattgtttt tgatgatgcg gatattgaag ctgtggtcga aggtgtacgt acttttggct
    36541 attacaatgc cggacaggat tgtactgcgg cttgtcggat ctacgcgcaa aaaggcattt
    36601 acgatacgct ggtggaaaaa ctcggtgctg cggtggcaac gttaaaatcg ggttcgccgg
    36661 atgacgagtc tacggagctt ggacctttaa gctcgctggc gcatctcgaa cgcgtcagca
    36721 aggcggtaga agaggcgaaa gcgacagggc acatcaaagt gatcactggc ggtgaaaagc
    36781 gcaagggtaa tggctattac tatgcgccga cgctgctggc tggcgcatta caggatgatg
    36841 ccatcgtgca aaaagaggta tttggtccgg tagtgagtgt tacgctcttc gacagcgaag
    36901 aacaggtggt gaactgggcg aatgacagtc agtacggact tgcatcttcg gtatggacga
    36961 aagatgtggg cagggcgcat cgcgtcagcg cgcggctgca atatggttgt acctgggtca
    37021 atacccattt catgctggta agtgaaatgc cgcacggtgg gcagaaactt tctggttacg
    37081 gcaaggatat gtcactttat gggctggagg attacaccgt cgtccgccac gtcatggtta
    37141 aacattaagg ataatattgc agatcgtaag agtatgagat gatcttgcgt actgtcagag
    37201 cactgatttc tctgacagta cgtgctgcgt gcaaaatcac tacatcaaat aaaccagcca
    37261 aatctcatag agataagagt aaacaaacca acagcagcaa agacatttat cagtactaca
    37321 gttctactgg aaacattcat ttttgccacc ttttatcatt gcccctatgt gtatagtacg
    37381 ggttaagaaa atccgcaata atactcattc tgaagatgga taagggcaag ttgctgtttg
    37441 atgatttttt ctcaaactgg tgaaaaaaac ttgatgcacg tcaaaaaatg acgcatattt
    37501 gcgcgcgttt tattcatctg gctggacgcc cgtacatgtc tctctatcaa cacatgcttg
    37561 ttttctatgc ggttatggca gcaatcgcat ttcttatcac ctggtttctt tctcatgata
    37621 agaaacgcat ccgtctctta agcgcctttc tggtgggggc aacatggccg atgagtttcc
    37681 cggtggcgct gttgttttct ctgttttaaa tcttatgcac tggcttgacg gcaccacgca
    37741 gacttatatc atttggatga atcgataaat ttcacaagtg gctaaggaga aagtatgtcg
    37801 catctggatg aagtcatcgc gcgcgtggat gccgcgattg aagagagcgt cattgcccat
    37861 atgaacgaat tattaatcgc cctgagcgat gacgcggagt taagtcggga agatcgctac
    37921 accctgcagc agcgtttgcg caccgctatt gctcatcacg gtcgcaaaca taaagaagat
    37981 atggaagcgc gccacgaaca gttaaccaaa ggcggtacca tcctctgatt aaaatgagcg
    38041 tctggccacc agccaggcgc caaccacaag taaaattgcg ccacaaattg ggccgatcaa
    38101 cgcccgaagt ggtacgccat ggctgcggaa gatatccagc accagaccgc caatcagctg
    38161 gctggcgacc agtactgcaa tagttgtcgc cgctcccaca ttctgatatc cgctgatact
    38221 ggcgaagaca aaaaacgatc ccagcaagcc aggtataagt gtccaccagc gcacgctgga
    38281 caccagttcg ccaaaccccg ccatgccttg tttaaaccat aaaatagaaa caaagagcac
    38341 aatccccacc agtgagttca gcaacatggc gatgagaatg gtagaggagg tctgggtgat
    38401 gcgcaccatc agcgtgttct ggactaccag accaatcccg gctgcaatca aaaaggcaag
    38461 ggtaagtgac tgattcatcc aatcgcgtcc ggttcagtgc gctcgtcgag ttgcaactgc
    38521 ataaatgtca gatccagcca gcgcccaaat ttagtgccta cctgcggcat ttgcgcggtg
    38581 acgacaaatc ccagcgactg gtgcagatgc agcgaggcct gattttgcga ttcgatcccg
    38641 gcgaccatga catgcttccc gcaatcccgc gcttcatcaa ttaatcggct taacaattta
    38701 cgccccagac ctttcccctg atgatcggga tggacataaa ccgaatgttc cacggtatgg
    38761 cgaaaaccat caaaactacg ccagtcgcca aacgaggcat atcccgtcac tacgccatct
    38821 tcttcactca ccagcaccgg ataacctgct atggtccgcg cttcaaacca ggcaatgcgg
    38881 ttatccgcat ccaccgtttg gtcattccag atagccgccg tatacaacac ggcgtggtta
    38941 taaatttccg caattgcagc acagtcggct ttgcgggcaa aacggatgga catgtctggc
    39001 ctcgataaaa taatgatgat gatgttatca atactatcga aaacagcccg aagcaacgga
    39061 ttcctctatg aaattattgc gtgttgcaga ggggatgtga cggctgcaaa caaaaccaat
    39121 cctgtgagcc tgctactatt gatggattca tgccaaaagc acaggagagc attatggggc
    39181 aacaaacgca gcgtaaccga tgttgggttc tgtcctcgcg tccgcatggc gcgccggttc
    39241 cttacgtctg gtttcggtaa atgcgctctt tgtcgcacca gcgcaagcat ttacagttcc
    39301 ggaagtggga aaacgtcaat taaatgaaat caatcggatc cgctacgaac tggagttaat
    39361 ggcagttgct ctggctgtcg aaaacctcac tatgcaaaaa aacgttgcta tgttgtgcca
    39421 gcaatacagc cgataaataa aatacgcccg gcaaataccg ggcttaaaga taattactca
    39481 aatcgccacg caatatttat gccgacacca taatttcgtc cgggcgcagg ttcgtaatat
    39541 cgcccgtttg actcattgac aatgacagaa ccaacgtatt ctttatcgaa taaattatcg
    39601 acacgaccaa ataaatccac ggttaaattg tggtaattgt atttataccc ggtgaataag
    39661 ccgacgagtg tataagaagg cgctttggcc gtattttcat catctgccat aatatcgccc
    39721 atataacgcg cttccgtgcc tgcataccaa ccatcttccg gtatataacc tatcgacgca
    39781 aagcccatat tacgggcgat ccctggcatc cggttaccgt tacaatcctg ttcattgcag
    39841 acattgctgc gataggtcgc atcaagccag gtccaggacg cttttacgcg aaaatctccc
    39901 gcgaaacgtt gatcccatgc cagttcagcg ccttgacgac gggtctttcc ggcatttttg
    39961 taagtcgtac gcccaccgct actgctatcg acaacaattt catcatcggt gtcggtctgg
    40021 aacaatgcga gactaagcag tccatcacca atacgcgttt tactgccgat ctcaattgta
    40081 tcgttggtgg agggttttaa accaaagttc ataccgcttt gtccatcagc gcgataagac
    40141 agttcattaa tcgtcggcgt ttcaaaaccg cgcccggctg ccagatagac attccaggca
    40201 tcggtcattg catattttaa cgatccggca ggtagccatt tgtgataact ggcatcaccg
    40261 ctgtcatcgc cgttacccgg agtaacgtaa tgatcgttgg aatcaaacca cacggagctg
    40321 tagcgcacgc cagcatccag cgaaagtttg tcggtcagtt gccactgggt ttgtaaatag
    40381 ggatcgacgt tccacatcaa attgcgttcg tcgcggcgca actcaccttt ttgcccgtat
    40441 tccggcacgc cgcgattcag gcggaagtta ttgtaaccct tgcggttttc actcatgttt
    40501 tcgtagttca ggccggttgt gaacgtgact ggaacgccca gttcaccacg gtgtgtccag
    40561 cggctgtcta tcccctggta atggcgttgc agggtaatca cgccgcccgc atgtgacggg
    40621 ttaagttgcg gggccatggg gattgactgg tactgggtcg tttctcgctc tccggcatac
    40681 atcatcacac tcatatcatc ttgtgcgctc aggctacgct catagcgcaa cccagcctga
    40741 gtttgcttga tggtttttcg catgtcgtac tgttctgcac gaggcgcttg ttgtggattc
    40801 gccttccatt ctgcttcggt tagcccacct gggtcatctg ctttgatatc cacactattg
    40861 aaaatcaggc ttaatttgct ggcgtcatca atgcgtacgc ccagtttggc attggctaaa
    40921 tttttctgtg cgccactatg gtcacgatag ccgtgggtcg taaaacgtgt ggttgcgacg
    40981 gtgtaatcga catcgccggg ttgtgtgccg tcgcccgttg cgcccgttgc tttcagccca
    41041 tagcgccagc tgccaaaact gccgtagtaa ctactggctt caatggttgg tggctgttgt
    41101 ccggtctggg tggtgacatt cattacaccg ccagacgcgt tgccatacag agcagagaag
    41161 gggccacgca gcacttccac attttgcaca ctgcttaaat cgatgttaga tgtctgccct
    41221 tgtccgtcgg gcatggtagc gggaataccg tccacataca ggcgaatacc gcgaattccg
    41281 taagtggagc gggagccaaa tccgcgaatc gacagctgta aatcttgcgc atagttctgt
    41341 cggttttgta cctgtaaacc aggcactccg gtcagtgatt cagacaagtt aatgcgcggt
    41401 gttgccaggc gcatctcctc gccatccacc acgctcacgg ctgctggggt atccagttct
    41461 gaaaccacct gcggtgcggc actgacaatc atggtctgtt catcagcggc aaaaacaacg
    41521 ggggaaagga caagcagtgc gggcaaaacg gtctgtcgga cggaaaaaat cttcatgaaa
    41581 aaagccaggt taagaatggg aaaacaccgt catggtaata aaattgtaaa tttatggaaa
    41641 acaaaacggc acaatacgtt aagtaattga gaaaattgta gtcataacgg taagaaatgc
    41701 tccacatttg agaaaataat gattaccatt cccatttata acaagggcgt aacgatgatt
    41761 acgcttagcg aagcattgtg aagcagcaaa aatatcggtt catcaaaggg agtcgtcatg
    41821 catttacgtc atctgttttc atcgcgcctg cgtggttcat tactgttagg ttcattgctc
    41881 gttgcttcat cattcagtac gcaggccgca gaagaaatgc tgcgtaaagc ggtaggtaaa
    41941 ggtgcctacg aaatggctta tagccagcaa gaaaacgcgc tgtggattgc cacttcgcaa
    42001 agccgcaaac tggataaagg cggcgtggtt tatcgtcttg atccggttac tctggaagtg
    42061 acgcaggcga tccataacga tctcaagccg tttggtgcca ccatcaataa cacgactcag
    42121 acgttgtggt ttggtaacac cgtaaatagt gcggtcacgg cgatagatgc caaaacgggc
    42181 gaagtgaaag gacgtctggt gctggatgat cgtaagcgca cggaagaggt acgcccgttg
    42241 caaccacgtg agctggtagc tgacgatacc acgaacaccg tttacatcag tggtattggt
    42301 aaagagagcg tgatttgggt cgttgatggc gagaatatca aactgaaaac cgccatccag
    42361 aacaccggta aaatgagtac cggtctggcg ctggatagca aaggcaaacg tctttacacc
    42421 actaacgctg acggcgaatt gattaccatc gacaccaccg acaataaaat cctcagccgt
    42481 aaaaagctgc tggatgacgg caaagagcac ttctttatca acatcagcct tgataccacc
    42541 aatcagcgtg catttatcac cgattctaaa gcggcagaag tgttagtggt cgatacccgt
    42601 aatggcaata ttctggcgaa ggttgcggca ccggaatcac tggctgtgct gtttaaccca
    42661 gcgcgtaatg aagcctacgt gacgcatcgt caggcaggta aagtcagtgt gattgacgcg
    42721 aaaagctata aagtggtgaa aacgttcgat acgccgactc atccgaacag tctggcgctg
    42781 tctgccgatg gcaaaacgct gtatgtcagt gtgaaacaaa aatccactaa acagcaggaa
    42841 gctacccagc cggacgatgt gattcgtatt gcgctgtaat agatatggcg agggtgaaaa
    42901 cggaaagaat tgcctgactt ttatcacgcc gcatccggca ccttcgtcag tagtctgaat
    42961 ttgcccggca cgtagccggg caattttgca attaactcgc cgtttcaggc ttaaacacaa
    43021 tgtcctgttt ttcttcatct tcctcgacga ctggcgcagt gctgtgaatt tcagcaacgc
    43081 gtttgcgcac accaaaccag cctataacca gcagaatacc gataatcggc agcgccgcga
    43141 tggtataagt cccgttcggg taatcgaacg ccatcagcac caggacactc agtaaaaaca
    43201 gtaatgtcag ccaggaagtg aagggcgcgc caggcagttt aaaactgaca tccgctgctt
    43261 tgccttcttt aatcgcttta cgcaggcgca tctggcaaac gatgataaac gcccatgaag
    43321 cgatgattcc cagcgacgcg aagttcaaca caatctcaaa tacgcgtgac ggcaccagat
    43381 agttgaggaa tacgccgacg acatacacaa ccagtgtcgc cagaatcccg gcatacggca
    43441 catgctgacg actcattttc gccataaaac tcggtgcgga accgcccatc gccatcgagc
    43501 gcagaatacg cccggtgcag tacagacctg aattcaggct ggagagggca gcggtcagca
    43561 ccacaatgtt cataatgctg cctatatatg gcacacccag tttagagaaa aacgtcacga
    43621 acggactttg ccccgcctga tatgcgctcc acggcaataa cataaccagc aacaccacgg
    43681 agccgacgta aaacagacca atacgccaaa tcacactgtt aatcgcttta ggtaccatgg
    43741 tctgcggatc tttacattct ccggccgctg tacccaccat ttcaatggag gcaaaagcaa
    43801 acactacgcc ctgaatcaac accagcgcag gcagcaaacc gtgggggaag aagccgccat
    43861 tatcggtgat taaatgaaag ccagtggtgt tgccatccag cggctgaccg ctaccgagga
    43921 acactgtacc cacgaccaaa aaggttacaa tggcgagcac tttaataagc gcaaaccaga
    43981 actccatctc cgcaaaccat ttcacaccga tcatattcat ggtgccaacg atggtcagtg
    44041 cagcgagcgc aaagacccac tgcggcacgc cgccaaacgc gccccagtaa tgcatataca
    44101 gagcgacggc ggtaatatca acaatccccg tcatcgccca gttgatgaag tacatccagc
    44161 cagcaacata agcggccttc tcacccaaaa actcacgggc ataagagaca aaactgccac
    44221 tggaagggcg gtgtaacacc agctcaccca atgcacgcaa aataaaaaac gaaaacaggc
    44281 cgcagatcaa atagaccagt gccagtgcgg gccccgccat ttgcagtcgc gcccccgcgc
    44341 ctaaaaacaa acccgtacca atcgcgccgc caatggcgat catctgtacc tggcgattgc
    44401 ccatcgcttt gtgatacccc tcttcgtggg cattaagcca gcggcgtttc gcggcgtgtt
    44461 gatctgaagt gtcggtgttg tgtttactca ttgctctccc tgattgcttt aatgaaaaag
    44521 tcatataagt tgccatgaac aatgtttatt catcctggcg atgacaagat tcaccattat
    44581 tctttatggg tataactgtc ggcgcagaat cctacctttt atcatgtaac gatgcaaaat
    44641 aatcccgcta taaatatgtg gggataaagt cggctggcga caaaatagca acctgatgaa
    44701 attatgacgg aaacaaccac tattggtttg atgatacctg actgcagtcc tactcttgcg
    44761 ccactcgttc gtcgctctga acgccagtgg ttgcagtgga cactggcgtg gttggctcaa
    44821 tgaatagtga ggggctagca catgatgggt ttcaggggta atacgccaaa taccaataca
    44881 attgctgatt aaatgagcca attgccgaca ttacagaaaa ggctaaaaaa tgtaagaggc
    44941 atgtagggtg ataaacgcgc tattgcccaa caggagtttc tggaattagt cct
//
LOCUS       DACADF010000019        43805 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.019, whole genome shotgun sequence.
ACCESSION   DACADF010000019 DACADF010000000
VERSION     DACADF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.019"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(99..3248)
                     /locus_tag="ISB30_001047"
     CDS             complement(99..3248)
                     /locus_tag="ISB30_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAP1988704.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHNHTVDHH"
     gene            complement(3271..4464)
                     /gene="acrA"
                     /locus_tag="ISB30_001048"
     CDS             complement(3271..4464)
                     /gene="acrA"
                     /locus_tag="ISB30_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAP1988705.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4606..5240
                     /gene="acrR"
                     /locus_tag="ISB30_001049"
                     /pseudo
     CDS             4606..5240
                     /gene="acrR"
                     /locus_tag="ISB30_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
     gene            5368..8730
                     /gene="mscK"
                     /locus_tag="ISB30_001050"
     CDS             5368..8730
                     /gene="mscK"
                     /locus_tag="ISB30_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAP1988706.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSALSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATQNGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNATEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8769..8933)
                     /gene="rsmS"
                     /locus_tag="ISB30_001051"
     CDS             complement(8769..8933)
                     /gene="rsmS"
                     /locus_tag="ISB30_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAP1988707.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDETSQSE"
     gene            complement(8947..9474)
                     /gene="priC"
                     /locus_tag="ISB30_001052"
     CDS             complement(8947..9474)
                     /gene="priC"
                     /locus_tag="ISB30_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAP1988708.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDESGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9544..9921
                     /locus_tag="ISB30_001053"
     CDS             9544..9921
                     /locus_tag="ISB30_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAP1988709.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10074..10625
                     /gene="apt"
                     /locus_tag="ISB30_001054"
     CDS             10074..10625
                     /gene="apt"
                     /locus_tag="ISB30_001054"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP1988710.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10754..12685
                     /gene="dnaX"
                     /locus_tag="ISB30_001055"
     CDS             10754..12685
                     /gene="dnaX"
                     /locus_tag="ISB30_001055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP1988711.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQGVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPDPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTFKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     gene            12738..13067
                     /locus_tag="ISB30_001056"
     CDS             12738..13067
                     /locus_tag="ISB30_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP1988712.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13067..13672
                     /gene="recR"
                     /locus_tag="ISB30_001057"
     CDS             13067..13672
                     /gene="recR"
                     /locus_tag="ISB30_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP1988713.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13782..15656
                     /gene="htpG"
                     /locus_tag="ISB30_001058"
     CDS             13782..15656
                     /gene="htpG"
                     /locus_tag="ISB30_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAP1988714.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETIISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            15837..16481
                     /gene="adk"
                     /locus_tag="ISB30_001059"
     CDS             15837..16481
                     /gene="adk"
                     /locus_tag="ISB30_001059"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP1988715.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16613..17575
                     /gene="hemH"
                     /locus_tag="ISB30_001060"
     CDS             16613..17575
                     /gene="hemH"
                     /locus_tag="ISB30_001060"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAP1988716.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17572..18531)
                     /gene="aes"
                     /locus_tag="ISB30_001061"
     CDS             complement(17572..18531)
                     /gene="aes"
                     /locus_tag="ISB30_001061"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HAP1988717.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGAIAEQRQYY
                     TLERRFWNADAPEMATRAYRVPTKYGQVKTRIFYPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVARRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSHLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18683..19987
                     /locus_tag="ISB30_001062"
     CDS             18683..19987
                     /locus_tag="ISB30_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAP1988718.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20117..21793)
                     /locus_tag="ISB30_001063"
     CDS             complement(20117..21793)
                     /locus_tag="ISB30_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAP1988719.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTR"
     gene            complement(22032..23252)
                     /locus_tag="ISB30_001064"
     CDS             complement(22032..23252)
                     /locus_tag="ISB30_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1988720.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23470..25122
                     /locus_tag="ISB30_001065"
     CDS             23470..25122
                     /locus_tag="ISB30_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAP1988721.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25160..25663)
                     /locus_tag="ISB30_001066"
     CDS             complement(25160..25663)
                     /locus_tag="ISB30_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988722.1"
                     /translation="MKFDHLVDVSFHNFVFNGKFDFVELGQTMAWLEQNFVEPDSKND
                     MGNGFYIWLFGNVEFHFENNKLYMIWCDYLSQIHLGKAIRFDKGILNNVAELNVTRVF
                     NMLVNEGADLQIRRQSPHTLLIHVNRSGVTLWFEADEEQPGDPQNYMLMAFGLTHRDY
                     DARFQYS"
     gene            complement(25660..26460)
                     /locus_tag="ISB30_001067"
     CDS             complement(25660..26460)
                     /locus_tag="ISB30_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988723.1"
                     /translation="MERITETLSIACADEIPDIALLRQTSREKVLHLHFASEAERYRE
                     EFGLVLKAQLDEFVVGMHSADPVIRIARLISLEEINRHQDFFEHCAKEYRKLATELIF
                     ALAEQLNVEMAENNPLVTFAPFKCNRKRKGKMGKWQYCFHGFHCTFENKKTEQNIEVP
                     LAYGFDFGDLDPYFFSGFIKSTPAWQPLPVAIYDDYHDGSRIIQQLLALGKLQKIPSP
                     IPQYTGVAAVDRSNVDIANFRSTLESRLHRCKLRWLLKHVKNSQRSER"
     gene            complement(26484..26963)
                     /gene="ybaK"
                     /locus_tag="ISB30_001068"
     CDS             complement(26484..26963)
                     /gene="ybaK"
                     /locus_tag="ISB30_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAP1988724.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(27167..27961)
                     /locus_tag="ISB30_001069"
     CDS             complement(27167..27961)
                     /locus_tag="ISB30_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988725.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEDQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            28094..28396
                     /locus_tag="ISB30_001070"
     CDS             28094..28396
                     /locus_tag="ISB30_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP1988726.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            28432..28773
                     /locus_tag="ISB30_001071"
     CDS             28432..28773
                     /locus_tag="ISB30_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAP1988727.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(28831..31335)
                     /gene="copA"
                     /locus_tag="ISB30_001072"
     CDS             complement(28831..31335)
                     /gene="copA"
                     /locus_tag="ISB30_001072"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAP1988728.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            31597..32529
                     /gene="glsA"
                     /locus_tag="ISB30_001073"
     CDS             31597..32529
                     /gene="glsA"
                     /locus_tag="ISB30_001073"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP1988729.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            32532..33824
                     /locus_tag="ISB30_001074"
     CDS             32532..33824
                     /locus_tag="ISB30_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP1988730.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPEVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKPLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            33949..34356
                     /locus_tag="ISB30_001075"
     CDS             33949..34356
                     /locus_tag="ISB30_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAP1988731.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(34357..35008)
                     /locus_tag="ISB30_001076"
                     /pseudo
     CDS             complement(34357..35008)
                     /locus_tag="ISB30_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35186..35644)
                     /locus_tag="ISB30_001077"
     CDS             complement(35186..35644)
                     /locus_tag="ISB30_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAP1988732.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(35641..36558)
                     /locus_tag="ISB30_001078"
     CDS             complement(35641..36558)
                     /locus_tag="ISB30_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAP1988733.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            36704..37381
                     /gene="fetA"
                     /locus_tag="ISB30_001079"
     CDS             36704..37381
                     /gene="fetA"
                     /locus_tag="ISB30_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAP1988734.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            37368..38147
                     /gene="fetB"
                     /locus_tag="ISB30_001080"
     CDS             37368..38147
                     /gene="fetB"
                     /locus_tag="ISB30_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAP1988735.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(38330..39184)
                     /gene="cnoX"
                     /locus_tag="ISB30_001081"
     CDS             complement(38330..39184)
                     /gene="cnoX"
                     /locus_tag="ISB30_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAP1988736.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RVLLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(39245..40054)
                     /gene="ybbO"
                     /locus_tag="ISB30_001082"
     CDS             complement(39245..40054)
                     /gene="ybbO"
                     /locus_tag="ISB30_001082"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAP1988737.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHREGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVGKVRHAFISEKPKMRYPVTLVTRAVMVLKRLLPGRVMDKILQG"
     gene            complement(40044..40667)
                     /gene="tesA"
                     /locus_tag="ISB30_001083"
     CDS             complement(40044..40667)
                     /gene="tesA"
                     /locus_tag="ISB30_001083"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HAP1988738.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            40638..41324
                     /locus_tag="ISB30_001084"
     CDS             40638..41324
                     /locus_tag="ISB30_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1988739.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            41321..43735
                     /locus_tag="ISB30_001085"
     CDS             41321..43735
                     /locus_tag="ISB30_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1988740.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQIAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLVLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVTVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WATLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNGDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKAGEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFVG"
BASE COUNT        10526 a        11455 c        11587 g        10237 t
ORIGIN      
        1 cgtatgggat cctgagttgg tggttcaata catccttaat gttcgtaggt tatgcataaa
       61 aaaggccgct tgcgcggcct tagtgattac acgttgtatc aatgatgatc gacagtatgg
      121 ttgtgctcga tatcttcatt cttgcggcta aagcggcggc gaaccaccac aaagaatacc
      181 ggaacgaaga agattgccag taccgttgcg gtcaccatcc cgcccattac accggtacct
      241 actgcgttct gcgcgccgga accagcacca gtactgataa ccagcggcat aacgccgagg
      301 ataaacgcca acgaggtcat caggattggg cgtaaacgca tccgcaccgc atcaagcgtc
      361 gcttcaatca gacctttacc ttctttatcc atcaagtctt tggcgaattc gacgataagt
      421 atcgcgttct tcgccgacaa accaatggtt gtgagcaggc ctacctggaa gtaaacgtca
      481 ttggtcaggc cacggaaggt ggcagccagc aacgcaccga taacccccag cggaacgacc
      541 agcataacgg agaacggaat cgaccagctc tcgtacagcg cagccagaca caggaacacg
      601 acaatcaacg aaatcgcgta cagtgaaggt gcctggttgc cggagagacg ttcctgatag
      661 gacatccccg tccagtcata gccaacaccg gtaggcagtt tgctcgccag ttgttccatc
      721 agctccattg cttcaccggt acttttaccc ggtgccgcct ggcctaagat ttccatagat
      781 ggcaggccgt tgtaacgttc cagacgcggc gaaccgtact cccaacgaga agaggagaac
      841 gccgagaacg gcaccatctg accatcagca gcacgaacat accagtcgcc gatatcatcc
      901 ggcagcatac ggtatttcgc ctctgacatg acgtaaactt tcttcacacg accgcggtcg
      961 ataaagtcgt tcacatagct accgccccat gcagcgccca gagtggtgtt aatgtcgttg
     1021 atagaaacac ccagcgcctg cgctttttcc tggtcgatat caatcttaaa ctgcggggta
     1081 tcttccagac cgtttggacg tacgctggtc aacatatcag ggtgcttcgc tgcttctgca
     1141 agcaactggt tacgcgcctg agtcagtttt tcgtgaccaa ggccagcctg gtcaatcagc
     1201 tcaaagtcaa agccggttgc ggtacccagt tccacgattg cgggcaggtt aaaggcgaaa
     1261 accatcgcat ctttgatttg cgagaaagca cgtgttgcac gcatggtaat cgcttcaact
     1321 ttgttttctt cgcccggacg atcggcccag tccttcaagg aaacgaacgc aataccggta
     1381 ttctgaccac gtcccgcaaa gccgaagccg ttaacggcga acaccgactc aacgttgttc
     1441 ttttctttgg tcagatagta attcgttacc tcattgagca ctttctgcgt acgttcctgc
     1501 gttgcacctg ctggcagctg aaccatggtc ataaatacgc cctggtcctc atctggcaag
     1561 aaggagcttg gcagacgcac gaacagatag gccatgccga ccacgatgat cagatacagc
     1621 accaggtaac gccccgtact gcgtagaata ccgcctacgc tgtcggtgta gtggtgcgtg
     1681 ctcttctcga acatgcggtt aaaccagccg aagaagcctt ttttaccttc cccgtgatcg
     1741 cctttggcaa tcggtttcag catggtggca caaagagccg gagtcaggat caacgccacc
     1801 agtaccgaca gcgccattgc tgaaacaatg gtaatagaga actgacgata gatagcacca
     1861 gtagaaccgc caaagaaggc catcggtacg aataccgccg acagtaccat cgcgataccg
     1921 accagagcgc cctgaatctg ccccatcgac ttacgggtgg cttcttttgg tggcaaacct
     1981 tcttccgcca tcacacgctc aacgttttct accacaacga tggcgtcatc caccaacagg
     2041 ccgatggcga gcaccatccc gaacattgtt agcgtgttta tcgagaagcc aaaggcggca
     2101 aggacggcaa aggtcccgag caataccacc ggtacggcaa tggtcggaat caacgtcgcg
     2161 cggaagttct gcaagaacag atacattacc aggaacacga ggatgatcgc ttcgaccagc
     2221 gttttaacca cttcgtgaat agagattttc acgaacggtg tggtgtcata cgggtaaaca
     2281 attttcagac ccgacgggaa gaacggttcc atcttcgcca gttcagcacg gattgccgca
     2341 gcggtatcca gcgcgttcgc accggtcgcc agcttgatcc ccagaccgga agccggttgg
     2401 ccgttaaact ctgcgatgat gtcgtagttc tcaccaccca gctcaatttt cgccacatca
     2461 cgcagcagca cgcgggaacc atcctgattc actttcagca ggattttgcc gaactcttca
     2521 gtagaggtca gacgcgtctg agcaataata gaggcgttaa gctgttggcc tttcaccggc
     2581 ggcgtaccac cgagctgacc agctgcaacc tgggcgttct gtgctttgat ggcggtaatg
     2641 acatcaaccg gcgttagctg gaatttgttc agttcattcg ggttcatcca gatacgcatc
     2701 gcgtactgtg aaccgaacaa ctgaacgtca cccacgcccg acgtacggct gatggcatct
     2761 ttcatattcg ccgccacgta gtcggagata tcctcctgcg tcatggtgcc atcggtgttg
     2821 ataacgccga caaccatcag gaagctgctg gatgatttct caacgctcac cccttgctgc
     2881 tgtacttctt gcggcagcaa cggcatcgcc agctgcagtt tgttctgcac ctgaacctgc
     2941 gcgatatccg catcagtacc agactcaaag gtcagggtga tctgcacggt acccgtggag
     3001 tcactgttag aggacatgta catcaggtta tcgataccgt tcatattctg ttcgataacc
     3061 tgtgtcaccg tgtcctgcac tgttttcgca tcagcgccag ggtaggaggc ggagatcgtt
     3121 actgccggcg gtgcaatcgt aggatattgc gccaccggca gtttgaggat cgccagcccc
     3181 cctgccaaca tgatgataat ggcgatcacc cacgcaaaaa ttgggcgatc gataaagaaa
     3241 ttaggcatgt cttaacggct cctgtttaag ttaagacttg gactgttcag gctgagcacc
     3301 gcttgcggct tgctggttat tatcagcggt aacttcttgt gcttttacct ggacaccagg
     3361 acgcactttc tgcagcccac ttattactac gcgatcgcct gctttcagac cttctgtcac
     3421 cagccactta tcgccgatag cctggcttgc aacgatcgga cgggtttcca ctttgtcatc
     3481 cgcgccaacc accagtacgg tggcatcgcc acgtggcgta cgggttacgc cctgttgcgg
     3541 gactaaaata gcgtttggat taagcccttc ttccagacgt gcacgcacga acatacccgg
     3601 cagcagagtg tgatccgggt tcgggaagat agcgcgtagg gtgatagacc cagtggtctg
     3661 atcaacggta acgtcagaga attccagcgt accgtcctgc gggaacttaa tgccgtcact
     3721 ggtgatcagc gacactttgg ctttgccgtt ctcttgtttc agcgtgccat tcgccagttc
     3781 ctgtttcagg cgcaggaagt cgttgctgga ctgggtcaca tcaacgtaga tcggatcaag
     3841 ttgctgcacg gttgccagcg cagtcgcctg accgttctgt accaatgcgc cttccgtcac
     3901 gtttgactta ccaatgcgac cactaatcgg agaggtgact ttggtgtaag ccagattgat
     3961 tcgcgcagtt tcaacggcag ctttcgccgc agttaccgca gcattcgcct gttgcgcatc
     4021 agccagagcc tgatcgtact cttgtttact gatgtactga gtaccgagca atttctgata
     4081 acgattcacc gtcaattgcg cgatattggc tgcagcctgg gctttcgcca gatcaccttt
     4141 cgcactgtcg tatgccgcct gataggtcgc aggatcaatc tgatagagag agacacctgc
     4201 ttcgatgtcg ctaccttctt tgaaattacg cttcaggata atcccgctaa cttgaggacg
     4261 aacttctgcg atccggtagg cactggtgcg acccggaagc tcggttgtga tctgcagagg
     4321 ttcagttttg actgttacta cgccaacggc gggcatctgc tggccacctt gttgggcctg
     4381 tttgtcgtca catcctgtta gggctaagct gcctgagagc atcagaacga ccgccagagg
     4441 cgtaaaccct ctgtttttgt tcatatgtaa acctcgagtg tccgatttca aattggtcaa
     4501 tggtcaaaag ttaataaacc cattgctgcg tttatattat cgtcgtgcta tggtacatac
     4561 attcacaaat gtatgtaaat ctaacgcctg taaattcacg aacatatggc acgaaaaacc
     4621 aaacaagaag cgcaagaaac gcgccaacac atcctcgatg tggctctacg tcttttctca
     4681 cagcaggggg tatcatccac ctcgctgggt gagattgcaa aagcagctgg cgttacgcgc
     4741 ggtgcaatct actggcattt taaagacaag tcggatttgt tcagtgagat ctgggaactg
     4801 tcagaatcca atattggtga actcgagctt gagtatcagg caaaattccc tggcgatcca
     4861 ctctcagtat taagagagat attaattcat gttcttgagt ccacggtgac agaagaacgg
     4921 cgtcgattat tgatggagat tatattccac aaatgcgaat ttgtcggaga aatggggcac
     4981 aacgtaatct ctgtctggaa agttatgacc gtatagaaca aacgttaaaa cattgtattg
     5041 aagcgaaaat gttgcctgcg gatttaatga cgcgtcgcgc agcaattatt atgcgcggct
     5101 atatttccgg cctgatggaa aactggctct ttgccccgca atcttttgat cttaaaaaag
     5161 aagcccgcga ttacgtcgcc atcttactgg agatgtatct cctgtgcccc acgcttcgta
     5221 accctgccac taacgaataa ccctgaatct gactccagga attttcctgg acattttcgt
     5281 cgttgctatt ctggttcact gcgtcgtgat attcttgcgg tttgactttt tcaggtcgtt
     5341 cttcaggttc agaaaccttc attcatcatg actatgttcc agtattacaa acgatcacgg
     5401 cattttgttt tttcagcatt tattgctttt gtttttgtct tgttatgcca gaacacggcg
     5461 tttgcacggg cgtcatcgaa tggtgattta ccgacaaaag cggacctgca ggcacaactt
     5521 gactcactga ataaacaaaa agatctttct gctcaggaca aactggtgca gcaggatctg
     5581 acagatacat tagccaccct cgataaaatc gatcgcgtaa aagaagagac agttcagcta
     5641 cggcaaaaag tcgctgaagc gccggaaaaa atgcgccagg cgaccgcggc gttaacagca
     5701 cttagtgatg tcgataacga cgaagaaacg cgcaaaattc tgagcgcgct ttcgttgcgc
     5761 cagctggaaa ctcgcgttgc ccaggcgctg gacgatttgc aaaacgcaca aaatgatctg
     5821 gcgtcttata acagccaact ggtttcgtta cagacgcagc ccgaacgcgt gcaaaatgcg
     5881 atgtataacg cttcgcagca gctgcaacaa attcgcagtc gtctggatgg gactgatgtc
     5941 ggcgagactg ccttacgtcc cagccagaaa gtgttaatgc aggtccagca ggctttgctg
     6001 aatgccgaga ttgaccagca gcgtaaaagc ctggaaggga acactgtctt gcaggatacc
     6061 ttgcaaaagc agcgtgatta cgtgacggcg aacagcgccc gtctggagca ccagttacaa
     6121 ctgttgcaag aagcggtaaa cagcaagcgc ctgactttaa ccgaaaaaac ggcgcaggaa
     6181 gctgtctccc cggatgaagc cgcgcgtatt caggctaatc cgctggtgaa gcaggaactg
     6241 gaaattaacc agcagttaag tcagcgtctg attaccgcga ctcaaaacgg taatcagttg
     6301 atgcagcaaa acattaaagt caaaaactgg ctggagcgtg cgctgcaatc ggaacgtaat
     6361 attaaggagc agattgccgt tctgaagggc agtctgctgt tgtcacgtat cctgtaccag
     6421 caacaacaaa cactcccctc ggcagatgaa ctggaaaaca tgaccaaccg catcgcggat
     6481 ttgcgtctcg aacagtttga agtcaaccag cagcgtgacg cgctgttcca gagcgatgcg
     6541 ttcgtcagca aactggaaga aggtcacacc aacgaagtca acagcgaagt tcacgatgca
     6601 ttattgcaag tagttgatat gcgtcgcgaa ttgctggatc agcttaacaa acagttgggt
     6661 aatcagctga tgatggccat taacctgcaa attaaccagc agcagttaat gagcgtatcg
     6721 aaaaatctga aatccatcct gacgcagcaa attttttggg tgaacagtaa ccgcccgatg
     6781 gactgggact ggattaaagc gttcccgcaa acgctgaaag atgaatttaa gtcgatgaaa
     6841 atcacggtga actgggaaaa agcctggccc gccgtattca tcgccttcct cgctggtttg
     6901 ccgctgctgt tgattgccgg gctgatccac tggcgtctgg gctggctgaa agcgtatcaa
     6961 caaaaactgg cttccgctgt gggttccctg cgtaacgaca gccagctcaa cacaccaaaa
     7021 gcgatcctta tcgacctgat ccgcgcactg ccggtgtgcc tgattattct cgctgtcggc
     7081 ctgattctgc tgactatgca gctcaatatc agcgagttat tgtggtcgtt cagcaaaaaa
     7141 ctggcgattt tctggctggt gtttggcctg tgctggaaag tgctggagaa aaatggtgtt
     7201 gccgtgcgtc actttggtat gccggaacag cagaccagcc actggcgtcg gcaaattgtc
     7261 cgcatcagcc tcgcgttgct gccgatccat ttctggtctg tggtggcaga actctccccg
     7321 ctgcatctga tggatgatgt gctggggcag gcgatgattt tcttcaacct gctgctgatt
     7381 gccttcctgg tgtggccgat gtgccgcgaa agctggcgtg ataaagagtc gcacaccatg
     7441 cgactggtca ccattaccgt gctgtcgatt atcccgattg cgttgatggt gctgaccgcc
     7501 acgggttact tctacaccac gctgcgcctc tccgggcgtt ggattgaaac cgtttatttg
     7561 gtgatcatct ggaacctgct gtaccagacg gtattgcgcg gcttaagcgt agcggcgcgg
     7621 cgtatcgcct ggcgccgtgc actggcgcgt cggcagaatc tggtgaaaga gggcgcagaa
     7681 ggtgccgaac cgccggaaga acccaccatt gcgctggagc aggttaacca gcagacgctg
     7741 cgtattacca tgttgctgat gtttgcgctg ttcggtgtca tgttctgggc aatttggtct
     7801 gatttgatca ccgtgttcag ctatctcgac agcattacgc tctggcatta caacgctact
     7861 gaagctggtg ctgcggtggt gaaaaacgtc accatgggca gtctgttgtt tgcgattatc
     7921 gcctcaatgg tggcctgggc gttgattcgc aacctgcctg gtttgctgga agtgctggtg
     7981 ctctcgcgac tgaatatgcg ccagggcgcg tcgtatgcca ttactaccat ccttaactac
     8041 atcattattg ccgttggtgc gatgacggtg ttcggatcgc tgggcgtctc gtgggataaa
     8101 ctccagtggc tggcggcagc attatccgta ggtcttggtt ttggtttaca agagatcttc
     8161 ggtaacttcg tttccggttt gatcattctg ttcgaacgtc cggtgcgcat tggcgacacg
     8221 gtaaccattg gtagcttctc gggtacggtc agcaagatcc gtattcgtgc gacaacgatt
     8281 accgatttcg atcgcaaaga agtgattatc ccgaacaaag cgtttgttac cgagcgtctg
     8341 atcaactggt cgttgactga cacgactacg cgtctggtga tccgtctcgg cgtagcctat
     8401 ggctccgatc tggaaaaagt gcgtaaagtg ttgctgaagg cggcgactga gcacccaagg
     8461 gtgatgcacg aaccaatgcc ggaagtcttc tttacggcat ttggtgccag cacgttggat
     8521 catgagctgc gtctgtatgt gcgtgaactg cgtgaccgta gccgtactgt cgatgagctg
     8581 aaccgtacta ttgatcagtt gtgccgtgaa aacgacatca acattgcctt taaccagctt
     8641 gaagtgcatc tgcacaacga gaagggcgat gaagtgacgg aagtgaaacg cgactacaaa
     8701 ggcgacgatc cgacgccagc ggtagggtaa ggacaataaa gggcgacagt gatgccgccc
     8761 tttatttttt actcgctttg tgaagtctca tcgctctgta acttcttttc ataatcgcgt
     8821 tttacaatat ccagcgcgcg cagcacggtg cgggctggga tctgattttc ttccagcagc
     8881 acaatcaaat cgacggccag tttgacatcg tcaggggcat tttccagtga catactctct
     8941 ccattgctag cgggttaaac gcgctaacct gttttcgatt ttttccagcg catggcggca
     9001 acgtgccagg cgcgcttcat aggcttccac ttcacgatgc agcgtctgct gttccacgag
     9061 atcggtcgct cgcgccagac gggctctacg ttcggcaacc atctcacgta gccgccgctc
     9121 aaaatcctga tgctgaatac gtttacgctg ccagcgggca attttcggtg gtgcgctatc
     9181 ccactcgcgt agcgaccagg ctgtggcttc acgcgctata gcctccagtt gcgccgccag
     9241 atgttctgcc agccaggcga cttgcggcag ctgttgctgc tcaaccgcat gacgaagcgc
     9301 agccaggtta tcgcccgact catcgagaca agcctgtagt gttgtcgcac gggtctgaaa
     9361 aaggtgcctg tcaaaacggg cacttagcgt ggcaaattgc gctaccgggg cacaacgctg
     9421 acgcagcgtg gcgagctgtc cttccagttt ttccagtagc agggcggttt tcacgataag
     9481 acctcaatga aaatgataat tgttatgcta aagtagccac tctgtaagct gacaacactc
     9541 aatatgcaac gaatcatttt aatcatcatt ggctggctgg cggtagtgct gggtacgctg
     9601 ggcgtggtat taccggtatt accgacgacg ccgtttatcc tgctggcggc ctggtgcttt
     9661 gcccgttctt ccccgcgctt tcacgcctgg ttgctgtacc gctcatggtt tggcagctat
     9721 ctacgtttct ggcagaaaca tcatgcgatg ccgcgcggcg caaaaccacg ggcgattttg
     9781 cttattttga ttacgtttac gatttcgctg tggttcgtcc agatgccgtg ggtgcgcatc
     9841 atgttgctgg taattctcgc ctgtttgctt ttttatatgt ggcgaattcc ggtgattgat
     9901 gaaaagcaag aaaagcactg aagcacaaca atcgcagttg caattattgc gtacagccag
     9961 tacattctgg cgttttcgag cacaggcgca ggcggtcaaa ggttaaacaa ctgttacttt
    10021 tgatacgttc aaaacgcgcc gtgagtacca ccgtaacaag caggcataca cttatgaccg
    10081 cgactgcaca gcagcttgag tatctcaaaa atagcatcaa aagcattcag gactacccaa
    10141 aacccggcat tcttttccgc gatgtcacca gcttactgga agacccgaaa gcttacgctc
    10201 tcagcatcga cttgctggtt gagcgttaca aaaatgcggg cattaccaaa gtggtcggca
    10261 ccgaagcgcg tggcttcttg tttggcgctc cggtagctct gggtctgggc gttggctttg
    10321 taccggtccg taaaccgggc aaactgccgc gtgaaaccat cagtgaaact tacgacctgg
    10381 aatacggcac cgatcaactg gaaatccacg ttgatgccat caaaccgggc gacaaagttc
    10441 tggtggtgga cgatctgctg gcaaccggcg gcactatcga agcgaccgtt aaactgatcc
    10501 gtcgtctggg tggtgaagta gctgacgctg cgttcattat caacctgttc gatctcggcg
    10561 gtgaacagcg tctcgaaaaa cagggcatta ccagctacag ccttgtcccg ttcccgggcc
    10621 attaattatc gtcagtcttg cgctgctcac gctacgggca gcacaagatg tgcattcagc
    10681 ctcgccgttc tgacggggct gtgttagcat taccccttcg tgaatccacc ttccagcgtt
    10741 tcagagcctg ccaatgagtt atcaggtctt agcccgaaaa tggcgcccac aaacctttgc
    10801 tgacgtcgtc ggccaggaac atgtgctgac cgcactggcg aacggcttgt cgttagggcg
    10861 tattcatcat gcttatcttt tttccggcac ccgtggcgtc ggaaaaacct ctatcgcccg
    10921 actgctggcg aaggggctaa actgcgaaac cggcattacc gcgacgccgt gcggcgtgtg
    10981 cgataactgt cgtgaaatcg agcaggggcg ctttgtcgat ctgattgaaa tcgacgccgc
    11041 ctcgcgtacc aaagttgaag atacccgcga cctgctggat aacgtccagt acgctccggc
    11101 gcgtggtcgt ttcaaagttt atctcatcga cgaagtgcat atgctgtcgc gtcacagctt
    11161 taacgcactg ttaaaaaccc ttgaagagcc gccagagcac gttaagtttt tgctggcgac
    11221 gaccgatccg cagaaattgc cggtgacgat tttgtcacgc tgtctgcaat ttcatctcaa
    11281 ggcgctggat gtcgagcaaa ttcgccatca gcttgagcac atcctcaacg aagaacatat
    11341 cgctcacgag ccgcgggcgc tgcaattgtt ggcacgcgcc gctgaaggca gcctgcgaga
    11401 tgccttaagt ctgaccgacc aggcgattgc cagcggtgac ggccaggttt caacccaggg
    11461 ggtcagtgcg atgctgggta cgcttgacga cgatcaggcg ttatcgctgg ttgaagcgat
    11521 ggtcgaggcc aacggcgagc gcgtaatggc gctaattaat gaagctgctg cccgtggtat
    11581 cgagtgggaa gcgttgctgg tggaaatgct cggtctgttg catcgtattg cgatggtaca
    11641 actttcgcct gctgcacttg gcaacgacat ggccgccatc gagctgcgga tgcgtgaact
    11701 ggcgcgcacc ataccgccga cggatattca gctttactat cagacgctgt tgattggtcg
    11761 caaagaatta ccgtatgcgc cggaccgccg tatgggcgtt gagatgacgc tgctgcgcgc
    11821 actggcgttc catccgcgta tgcctctgcc tgatccagaa gtgccacgcc agtcctttgc
    11881 gcccgtcgcg ccaacggcag taatgacgcc aacacaggtg ccgccgcaac cgcaatcagc
    11941 gccgcagcag gcaccgactg taccgctccc ggaaaccacc agccaggtgc tggcggcgcg
    12001 ccagcagttg cagcgcgtgc agggagcaac caaagcaaaa aagagtgaac cggcagccgc
    12061 tacccgcgcg cggccggtga ataacgctgc gctggaaaga ctggcttcgg tcaccgatcg
    12121 cgttcaggcg cgcccggtgc catcggcgct ggaaaaagcg ccagccaaaa aagaagcgta
    12181 tcgctggaag gcgaccactc cagtgatgca gcaaaaagaa gtggtcgcca cgccgaaggc
    12241 gctgaaaaaa gcgctggaac atgaaaaaac gccggaactg gcggcgaagc tggcggcaga
    12301 agccattgag cgcgacccgt gggcggctca ggtgagtcaa ctttcgctac caaaactggt
    12361 cgaacaggtg gcgttaaatg cctggaaaga ggagagcgac aacgcagtat gtctgcattt
    12421 gcgctcctct cagcggcatt tgaacaaccg cggcgcacag caaaaactgg ctgaagcgtt
    12481 gagtacgttc aaaggttcaa cggttgaact gactatcgtt gaagatgata atcccgcggt
    12541 gcgtacgccg ctggaatggc gtcaggcgat atacgaagaa aaacttgcgc aggcgcgcga
    12601 gtccattatt gcggataata atattcagac cctgcgtcga ttcttcgatg cggagctgga
    12661 tgaagaaagt atccgcccca tttgatcgta agcacagctt acgttcgtca tccttaacgt
    12721 gattgagaga gaaacctatg tttggtaaag gcggtctggg taacctgatg aagcaagccc
    12781 agcagatgca agaaaaaatg cagaaaatgc aggaagagat cgcgcagctg gaagtcaccg
    12841 gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg tgcacacaac tgccgtcgcg
    12901 tagagatcga cccgagcctg ctggaagacg acaaagagat gctggaagac ctggtggctg
    12961 cagcattcaa cgacgcagca cgtcgtattg aagaaacgca gaaagaaaaa atggcctctg
    13021 tatcctccgg aatgcagctg ccgcctggct ttaagatgcc gttctgatgc aaaccagccc
    13081 gctgttaaca cagcttatgg aagcactgcg ctgtctgccg ggcgttggcc cgaagtcggc
    13141 gcagcgtatg gcgttcacgc tgcttcagcg cgatcgtagc ggcgggatgc gtctggcgca
    13201 ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc gattgtcgca ccttcaccga
    13261 acaggaagtc tgtaacatct gttcgaatcc acgtcgtcag gaaaacggtc aaatctgcgt
    13321 ggtggagagt ccggcggaca tttacgccat cgagcagacg gggcagtttt caggtcgtta
    13381 ttttgtgttg atgggacacc tgtcaccgct ggacggcatc ggtcctgatg atatcgggct
    13441 tgatcgtctg gaacagcgtc tggcagagga aaaaatcact gaagtgatcc tcgccaccaa
    13501 ccccacggtt gaaggtgaag ctaccgctaa ctacattgcc gagctttgtg cgcaatatga
    13561 cgtggaagcc agccgaatcg ctcatggcgt accggttggc ggcgaactgg aaatggtcga
    13621 tggcaccacg ctgtcacact cccttgccgg gcgtcataag attcgttttt aagcaaacga
    13681 gagcaggatc acctgctctc gcttgaaatt attctccctt gtccccatct ctccctcatc
    13741 ctgtttttaa ccttaaaatg gcattattga ggtagaccta catgaaagga caagaaactc
    13801 gtggttttca gtcagaagtg aaacagcttc tgcacctgat gatccattct ctctattcca
    13861 ataaagaaat cttcctgcgt gagcttatct ctaacgcctc cgatgcggcg gacaagctgc
    13921 gtttccgcgc gctttctaac ccggacctgt acgaaggtga tggtgaacta cgcgttcgtg
    13981 tctctttcga taaagacaag cgcacgctga ccatctccga taacggcgtg gggatgaccc
    14041 gcgacgaagt gattgaccat ctggggacta tcgctaaatc cggcaccaaa tcattcctcg
    14101 aatccctggg ttctgaccag gcgaaagaca gccagctgat cggtcagttt ggtgttggtt
    14161 tctactctgc gtttatcgtg gctgacaaag tgaccgtgcg tactcgcgcg gcaggcgaaa
    14221 aaccagaaaa cggcgtcttc tgggaatcgg ctggcgaagg tgaatacacc gttgccgaca
    14281 tcactaaaga agatcgtggt actgaaatca ccctgcatct gcgtgaaggc gaagacgagt
    14341 tcctcgatga ctggcgtgtg cgttccatca tcagcaaata ttccgaccat atcgcgctgc
    14401 cggtagagat cgaaaaacgc gaagagaaag acggcgaaac cattatctcc tgggagaaaa
    14461 tcaacaaagc gcaggcgctg tggactcgta acaagtcgga aatcaccgat gaagagtaca
    14521 aagagttcta caaacacatc gcccacgact ttaacgatcc gctgacctgg agccacaacc
    14581 gcgttgaagg taagcaggag tacaccagcc tgctgtacat tccgtctcag gcaccgtggg
    14641 atatgtggaa ccgcgatcat aaacacggtc tgaaactgta tgttcagcgt gtgttcatca
    14701 tggacgacgc agaacagttc atgccgaact atctgcgctt cgtgcgtggt ctgattgact
    14761 ccagcgatct gccgctgaac gtttcccgtg aaatcctcca ggacagcacg gtaacgcgta
    14821 acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct ggaaaaactg gcgaaagacg
    14881 acgcggaaaa ataccagacc ttctggcaac agtttggcct ggtactgaaa gaaggtccgg
    14941 cggaagattt cgctaaccag gaagcgatcg ccaaactgct gcgttttgct tctacccata
    15001 ccgattcttc tgcgcagacc gtgtctctgg aagactacgt ttcccgcatg aaagaagggc
    15061 aggagaaaat ctactatatc accgcagaca gctacgcggc agcgaagagc agcccgcacc
    15121 tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct ttccgaccgc atcgatgagt
    15181 ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt ccagtctgtg tctaaagttg
    15241 acgagtcgct ggaaaaactg gctgacgaag ttgatgagag cgcgaaagaa gcggagaaag
    15301 cactgactcc gttcatcgac cgtgtgaaag ccctgctcgg cgagcgcgtg aaagatgtcc
    15361 gtctgactca ccgtctgacc gatacgccag cgatcgtttc gaccgacgcg gacgaaatga
    15421 gcactcagat ggcgaaactg ttcgctgcgg cgggccagaa agtgccagaa gtgaaataca
    15481 tcttcgaact gaacccggat cacgtactgg tgaaacgtgc ggcggatact gaagatgaag
    15541 cgaagttcag cgagtgggta gaactgctgc tggatcaggc gctgctggca gaacgcggca
    15601 cgctggaaga tccgaacctg tttattcgtc gtatgaacca gctgctggtt tcctgatgtc
    15661 atgccggatg accttcgtgt catccggcat ttttcttttc ttcatctgca ctttccgcaa
    15721 attatcttgc cattaaccgt ttcagcctca ggtgcctttc ttgaggcaat cgcctgttgg
    15781 tggtatcgtt tatcgctttt tcaaaaaatt cgacacattt taaggggatt ttcgcaatgc
    15841 gtatcattct gcttggcgct ccgggcgcgg ggaaagggac tcaggctcag ttcatcatgg
    15901 agaaatatgg tattccgcaa atctccactg gcgatatgct gcgtgctgcg gtcaaatctg
    15961 gctccgagct gggtaaacaa gcaaaagaca ttatggatgc tggcaaactg gtcaccgacg
    16021 aactggtgat cgcgctggtt aaagagcgca ttgctcagga agactgccgc aacggtttcc
    16081 tgttggacgg cttcccgcgt accattccgc aggcagacgc aatgaaagaa gcgggcatca
    16141 atgttgatta cgttctggaa ttcgacgtac cggacgaact gattgttgac cgtatcgtag
    16201 gccgccgcgt tcacgcgccg tctggtcgtg tttatcacgt taaattcaat ccgccgaaag
    16261 tagaaggcaa agacgacgtt accggtgaag agctgactac ccgtaaagac gatcaggaag
    16321 agaccgtacg taaacgtctg gttgaatacc atcagatgac tgcaccgctg atcggctact
    16381 actccaaaga agcggaagcg ggtaacacca aatacgcgaa agttgacggc accaagccgg
    16441 tggctgaagt tcgcgctgat ctggaaaaaa tcctcggcta atcaagccgc agcagatctg
    16501 ctttcgggca ggtctgctca tcctcctcag gcaatacctc ttacagcaat tagttcttct
    16561 tcttcacttt tccgctacaa ttatcaacaa gttgaatcga taagaggcgg taatgcgtca
    16621 gactaaaacc ggtatcctgc tggcaaacct gggtacgccc gatgccccca cacctgaagc
    16681 ggtaaaacgc tatctgaaac aatttttaag cgacagacgc gtggttgata cctcacggtt
    16741 gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg cgttcgccgc gtgtggcgaa
    16801 gctgtatgcc tctgtctgga tggaaggtgg ctcgccgctg atggtttaca gccgccagca
    16861 acagcaggcg ctggcgcaac gtttaccgga gacgcccgtg gcgctgggaa tgagctacgg
    16921 ctcgccatca ctggaaagcg ccgtagatga acttctggca gagcatgtag atcatattgt
    16981 ggtgctgccg ctttatccgc aatactcctg ttcaacggtc ggtgcggtat gggatgaact
    17041 ggcacgcatt ctggcgcgca aacgtagcat tccggggata tcgtttattc gtgattacgc
    17101 cgataaccac gattatatta atgcactggc gaacagcgta cgtgcttctt ttgccaaaca
    17161 tggcgaaccg gatctgctgt tgctctctta tcatggcatt ccccagcgtt atgcagatga
    17221 aggcgatgat tacccgcaac gttgccgcac aacaactcgc gaactcgctt ccgcactgga
    17281 gatggcaccg gaaaaagtga tgatgacctt tcagtcgcgc tttggccggg aaccctggct
    17341 gatgccttat accgacgaaa cgctgaaaat gctcggagaa aaaggcgtgg gtcatattca
    17401 ggtgatgtgc ccgggctttg ctgcggattg tctggagacg ctggaagaga ttgccgagca
    17461 aaaccgtgaa gtcttcctcg gcgctggcgg gaaaaaatac gaatatattc cggcgcttaa
    17521 tgctacaccg gagcatatcg aaatgatggc taatcttgtt gccgcgtatc gctaaagctg
    17581 agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg gcggttttca tcatccgtga
    17641 ataatgcaaa aaggcgtgca gcgtgcctgg gtagagtttg aactcacagg gctgctgatg
    17701 cgccgctaac gtctggtaaa gcagatggct gtcatccagc agcggatcga actccgcccc
    17761 ggcaataaaa cagggcggaa cttcgcgagt gagatcatta ttaaacaggc agtaatatgg
    17821 cgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca tacatctgca aatcctgttg
    17881 cgttaagcca tcccagacac cacccaacag acgacgagcc acggaatccc gtaatccgta
    17941 aagcccatac cacagcaaaa cgcccgcaac tttaccgcaa tcgatctgtt tatcgcgcaa
    18001 ccacaacgca ctggcgagcg ccagcatggc acctgcggaa tcaccggcaa aaccaatgcg
    18061 ggacatattg atttgataat cctccgcctg ctggtggaaa taacaacagg cagccacaat
    18121 ttcctctatc gcttgcggaa aacgcgcttc aggtgaaagg gtgtaatcaa tgccaatcac
    18181 cgtacattgg ctgtagcttg ccagcaggcg catgatgcga tcgtgggtat cgagattgcc
    18241 gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc gggctatctg gctgcggata
    18301 aaagatacgt gttttcacct gcccatattt tgttggaacc ctgtaagctc tggttgccat
    18361 ttctggagcg tccgcattcc agaatcggcg ctcaagcgtg taatactgtc gttgctcagc
    18421 aatcgctccc gttgcaggcc agggcggtaa atccggctga agagtattca caacggtctt
    18481 catttcagca gagataaggt ccagaacggg taatttgttt tccggcttca tgcaaaaact
    18541 cctttcaaat tacgtcattg taaggaacca ctgccatgaa aatgcgatcc cgcctgctga
    18601 tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg tgttaacatt cgccgctcag
    18661 ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa cgtaaatcca aacattactt
    18721 ccccgtaaac gcacgcgatc cgctgcttca gcaattccag ccagaaaacg aaaccagcgc
    18781 tgcctgggta gtgggtatcg atcaaacgct ggtcgatatt gaagcgaaag tggatgatga
    18841 attcattgag cgttatggat taagcgccgg gcattcactg gtgattgagg atgacgttgc
    18901 cgaagcgctt tatcaggaac taaaacagaa aaacctgatt acccatcagt ttgcgggtgg
    18961 caccattggt aacaccatgc acaactactc ggtgctcgcg gacgaccgtt cggtgctgct
    19021 gggcgtcatg tgcagcaata ttgaaattgg cagctatgcc tatcgttacc tgtgtaacac
    19081 ctccagccgt accgatctta actatctaca aggcgtggat ggcccgattg gtcgttgctt
    19141 tacgctgatt ggcgagtccg gggaacgtac ctttgctatc agcccgggcc acatgaacca
    19201 gctgcgggct gaaagtattc cggaagatgt gattgccgga gcctcggcac tggttctcac
    19261 ttcttatctg gtgcgttgca agccgggtga acccatgccg gaagcaacca tgaaagccat
    19321 tgagtacgcg aagaaatata acgtaccggt ggtgctgacg ctgggcacca agtttgtcat
    19381 tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac cacgtctcta tccttgcgat
    19441 gaacgaagat gaagccgaag cgttgaccgg agaaagcgat ccgttgttgg catctgacaa
    19501 ggcgctggac tgggtagatc tggtgctgtg caccgccggg ccaatcggct tgtatatggc
    19561 gggctttacc gaagacgaag cgaaacgtaa aacccagcat ccgctgctgc cgggggctat
    19621 agcggaattt aatcagtatg agtttagccg cgccatgcgc cacaaggatt gccagaatcc
    19681 gctgcgtgtc tattcgcaca ttgcgccgta catgggcggg ccggaaaaaa tcatgaacac
    19741 taatggagca ggggatggcg cactggcagc gttgctgcat gacattaccg ccaacagcta
    19801 ccaccgtagc aacgtaccaa actccagcaa acataaattc acctggttga cgtattcgtc
    19861 attagcgcag gtgtgtaaat atgctaaccg tgtgagctat caggtactga accagcattc
    19921 acctcgttta acgcgcggct tgccggagcg tgaagacagc ctggaagagt cttactggga
    19981 tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt cacgccgcat ctggcatttt
    20041 gctctcaatg cctgatgcga cgctggcgcg tcttatcagg cctacatatc cgcgcctatt
    20101 atccggcaac caccgtttac ctcgtcacca cctcacccgc cggtggcgtt tccagcagtt
    20161 ccagcatagt acgggcaatt tcacgctcac ccatcactac ctgattcgca ccacgttcgg
    20221 tgatatacgc cacttcatcg tcataatggg cgcgggcaat aatctcaata tccggatttt
    20281 tcgcgcgggc agatgccaca atctcgcccg cttcatagcc gttgggaatc gtcaggatca
    20341 gccattttgc acattccaga tgcgccagtt gcataatttc ttcgttagcc gcattgccca
    20401 atactgcgcg gaccccgcgc tcgcgcagct catcaacacg ggttcgtgac gtctcaatca
    20461 ccaccagcgg aatatcagag gcgagcaatt tctcccccag caggctgcct acacgaccgt
    20521 aacccaccag tagcgcatgg ttgcaaatat ccactgggat ctgcttctct tcttcgattg
    20581 cctcttccag cgtctgctct tccagcgttt cggtcttcgc cagatatttc tccagtagtg
    20641 cgaacagtac cgggttgagc ataatcgaca ggatcgcccc tgccagtacc aggttttgtc
    20701 cggcctgcgg cagtaaattc aatgccattc ctagtcccgc caggataaac gcgaactcac
    20761 caatctgcgc caggctggcg gcgatggtta acgccgtgcg ctgggagtga ccaaacaaac
    20821 gcacaaggaa cagggccgcg accgatttac caaacagaat aattgccagc gtcgccagta
    20881 ctgccagcgg ttgctgaatc agaattaacg gatcaaacaa catcccgacg gagacaaaaa
    20941 acagcaccgc aaacgcgtcg cgcaatggca gtgtatcgtg ggcggcacgg tgactcagtt
    21001 cagactcgtt cagtaccatc ccggcaaaga acgcaccgag tgcaaaggag acatcaaaca
    21061 gctctaccgc accaaaggca atccctaacg ccagcgccag caccgacagg gtaaacagct
    21121 cgcgggaacc ggttgctgcg ctgcgtgcca taatccacgg caccagacgg cgacctacca
    21181 gcatcataat ggcgataaat gcgatcacct tgccgatggt gatccccata tcgactgcaa
    21241 gagtggcaaa gcccacatcg ccctgttcca tcattcctgc taccgcgggt agcaatacca
    21301 gtgtcaggac catcaccagg tcttccacaa tcaaccaacc gatggcgatt tgcccacgct
    21361 gactgtcaat taattgccgt tcttcaagtg cgcgcagtaa caccacggta ctggcggtgg
    21421 aaagacataa gccgaacacg ataccggtca ttaacgacca gcccagcacg gcggagagcg
    21481 ccatacccag cagcgtcgcc acggctatct gggcgatcgc gccgggaatg gcgatggact
    21541 ttaccgccat caaatccttg agcgaaaaat gcaaaccgac gccgaacatc agcaaaataa
    21601 cgcccagttc agccagttcc ggcgcaagct tagtatcggc aacaaagccc ggagtgaatg
    21661 gtcctgccag cacacccgct aacagatatc ccaccagagg agaaatacgt agtttattgg
    21721 ccagcatgcc gaggataaag gcgagcacaa ggccgccaac aatggtggtg ataagcgggg
    21781 tggcgtgatg cattccgtct ccttttcctg gtggttattg tccatttttg gccgggaaaa
    21841 ccaaaattac aggtaatagt ttatgacaat ttcattgatg atgtttatga ataattgttg
    21901 aattttgcag aaaaatggaa ttagctgcaa aaaaaagcac ggatcggaaa acagaaggcg
    21961 gtttaacaga ggaaaggctt atggcgactg taaaacgatg cagccaaagt ttgcctttgg
    22021 ctgcaatgaa atcagtcttt atgccggtta tcaggcagga atatggtcaa catccccaat
    22081 agtggcagga aagcacagat tttatagact aactctatgc tggtgtggtc ggcgataagc
    22141 cccagaactg ccgcccccag acctcccatg ccgaaagcaa aaccgaaaaa tagtccagaa
    22201 accataccga tacgtcccgg aagcagctcc tgggcataga ccagaatggc agagaatgcc
    22261 gaagcgagga taaatccaat aatcaccgtt aaaacccccg tccagtgcag gctggcgtag
    22321 ggtaaaatca gcgtaaacgg cgcaacgccg aggatagagc cccaaatcac atatttccgt
    22381 ccaattttat cccctacagg cccgccaatc accgtacctg ccgcaacggc aaacaggaag
    22441 gcaaacaaat gaagctgagc attctggata gataatccga atttttgcat cagataaaag
    22501 gtgtaatagc tgctgatgct cgccatatag aaatatttcg agaaaatgag gattaacaga
    22561 atgctgaccg ccagaacaac tttattgcgc ggcagtggat tgataatcgt cgctttgggt
    22621 tttcctttat tcattcggtg ctgtgccgag taccaacggc tgatttgcgc caacaccacg
    22681 atcgccagca gtgccgcaag cacaaaccag gcaacgttgc ctttgccata aggcgcgata
    22741 atcaccgccg ccagcaaggg gcccagggaa ctgccaaagt tgccgccgac ctgaaatata
    22801 gattgcgcca ggccatgacg cccgccggaa gccatacggg ccacgcgaga agattccgga
    22861 tgaaagaccg atgaaccggt accgaccagc gccgctgcca gcagaactgc gccgaaactg
    22921 cccgccagcg ccagcagcac cagaccactt aaggtaaagc acatgccaat cggcaacgac
    22981 cacggcatcg gatatttatc ggtccagtag ccgaccaccg gttgcagtag cgaagaggcg
    23041 agctggaagg tgagggttat catgccaatc tgcataaatg tcagagaaaa ttctgactga
    23101 agcagtggat aaatcgccag aatcagcgat tggatcatgt cgttcagcag atgtgagagg
    23161 ctgatagcac ctaaaatacc aaacgatgtt cgggccttag tcgttgacgc agccgcgccc
    23221 gccacaggct gggtttgttc actcattgcc ataggaaagt cactttttca gggttgcgat
    23281 gtaaagaatg atcttatttg tgattattac ccgactaaca tacctgtatg cgccgcctga
    23341 aggaagtctc aacacggaat acagaatttc taatctggat gcagatttat cttcaccgga
    23401 cgcagacttg tttatgatgt cgcgtcatac tatttttcaa cacgttgaaa tcaggtcagg
    23461 gagagaagta tgaaattatt gcagcggggc gtggcgttag cactgttaac cacatttaca
    23521 ctggcgagtg aaactgctct ggcgtacgag caggataaaa cctacaaaat tacagttctg
    23581 cacaccaatg atcatcatgg gcatttttgg cgcaatgaat atggtgaata tggtctggcg
    23641 gcgcaaaaaa cgctggtgga tggtatccgc aaagaggttg cggctgaagg cggtagcgtg
    23701 ctgctacttt ccggtggcga cattaacact ggcgtgcccg agtccgactt acaggatgcc
    23761 gaacctgatt ttcgcggtat gaatctggtg ggctatgacg cgatggcgat cggtaatcat
    23821 gaatttgata acccgctcac cgtattacgc cagcaggaaa agtgggctaa gttcccgttg
    23881 ctttccgcca atatctacca gaaaagtacc ggcgagcgcc tgtttaaacc atgggcgctg
    23941 tttaagcgtc aggatctgaa aattgccgtt attggcctga cgacggatga cacagccaaa
    24001 attggtaatc cggaatattt cactgatatc gaatttcgta agcccgctga tgaagcgaag
    24061 ctggtgattc aggagctgca acagacagaa aagccagaca ttattatcgc ggcgacccat
    24121 atggggcatt acgacaatgg tgagcacggc tctaacgcac cgggcgatgt ggagatggca
    24181 cgcgcgctgc ctgccggatc gctggcgatg atcgtcggtg gtcactcgca agatccggtc
    24241 tgtatggcgg cagaaaacaa aaaacaggtc gattacgtgc cgggtacgcc atgcaaaccg
    24301 gatcaacaaa acggcatctg gattgtgcag gcgcatgagt ggggcaaata cgtgggacgg
    24361 gctgattttg agtttcgtaa tggcgaaatg aaaatggtta actaccagct gattccggtg
    24421 aacctgaaga agaaagtgac ctgggaagac gggaaaagcg agcgcgtgct gtacacccct
    24481 gaaatcgctg aaaaccagca aatgatctcg ctgttatcac cgttccagaa caaaggcaaa
    24541 gcgcagctgg aagtgaaaat aggcgaaacc aatggtcgtc tggaaggcga tcgtgacaaa
    24601 gtgcgttttg tacagaccaa tatggggcgg ttgattctgg cagcacaaat ggatcgcact
    24661 ggtgccgact ttgcggtgat gagcggaggc ggaattcgtg attctatcga agcaggtgat
    24721 atcagctata aaaacgtgct gaaagtgcag ccattcggca atgtggtggt gtatgccgac
    24781 atgaccggta aagaggtgat tgattacctg acagccgtcg cacaaatgaa gccagattca
    24841 ggtgcctacc cgcaatttgc caacgttagc tttgtggcga aagacggcaa actgaacgac
    24901 ctgaaaatca aaggcgaacc ggtcgatccg gcgaaaactt accgtatggc gacattaaac
    24961 ttcaatgcca ccggcggtga tggctatccg cgccttgata acaaaccggg ctatgtgaat
    25021 accggcttta ttgatgccga agtgctgaaa gcgtatatcc agaaaagctc gccgctggat
    25081 gtgagtgttt atgaaccgaa aggtgaggtg agctggcagt aatgaacccc gtcgcggcgt
    25141 catgatatga cgccgccatt tatgaatatt gaaatcttgc atcgtaatcg cgatgtgtca
    25201 aaccaaatgc catcagcata taattctgcg gatcaccagg ctgttcttca tctgcttcaa
    25261 accaaagtgt cacgcctgag cgattgacgt ggattaagag tgtatggggc gattgacggc
    25321 ggatttgcag atcagcgcct tcgttaacaa gcatgttaaa tacacgcgta acgttcaact
    25381 cagcgacgtt attcaatatt cctttatcaa atcggatcgc tttgcccaaa tggatttggc
    25441 tcagataatc gcaccaaatc atatatagct tgttgttttc gaaatgaaac tcaacattac
    25501 caaatagcca gatataaaaa ccatttccca tatcattttt agagtccggc tcaacaaagt
    25561 tttgttccaa ccatgccatt gtttgcccca gttcgacaaa atcgaatttt ccgttaaaga
    25621 caaaattgtg gaagctaaca tcaacaagat ggtcaaattt catctttcgc tcctttgtga
    25681 attttttaca tgcttcaaaa gccagcgtaa tttacatcgg tgcaggcgac tctccagtgt
    25741 tgagcgaaaa tttgcgatgt cgacattgga ccgatctacc gcagcaaccc ccgtgtattg
    25801 tgggatcggt gaagggattt tttgtaactt tcccagcgcc agcagttgtt gaatgatcct
    25861 gctaccgtcg tggtaatcat cgtaaatagc cactggcaga ggctgccagg cgggggtcga
    25921 tttgataaat ccggaaaaaa aatacgggtc cagatcgccg aaatcaaagc cgtaggcgag
    25981 gggaacttca atattttgct ctgttttttt attctcaaat gtacaatgaa aaccgtgaaa
    26041 acaatactgc cactttccca ttttgccttt tcgcttacgg ttgcatttga agggggcaaa
    26101 ggtaactagc gggttatttt ccgccatctc aacgttcagt tgttcagcca atgcaaatat
    26161 aagctctgtc gccagcttac gatattcttt cgcgcagtgt tcgaaaaaat cctgatggcg
    26221 gttaatctct tcaagcgata ttaatctggc gatcctgata accgggtcgg cagaatgcat
    26281 gccaacaaca aattcatcga gctgtgcttt cagcaccagc ccgaattcct cacgatacct
    26341 ctctgcttcg ctggcaaaat gcagatgtaa taccttctct ctcgatgttt gccgaagtaa
    26401 ggcgatatcc ggtatctcat ctgcacaagc gatacttaat gtctctgtaa tcctttccac
    26461 atctcatctt ccgtgtgcat cacttaatcg cgacgggcga tatcagcaaa tttggcatcg
    26521 agtatctttg ccaggtcgct tgccgccagt tcgatatcca gtccgcgctt gccgccggaa
    26581 acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgttggcag acgttttttc
    26641 tgccccagtg ggctaatccc ccctaccaga taccccgtcg aacgctgcgc gaccatcgga
    26701 tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag atccagttga
    26761 cctgcgaccg gcgtaacagc cacggcaagg tgtttcatat caccgttcac tgcgaccagc
    26821 agcgttttgt agacctgatc cggattcaaa cctaattttt tgacgacttc atcgccaaaa
    26881 ttggtttcag caggatcgtg ctcgtaggtg tggatttgaa acgaaatctt gtttttttcg
    26941 agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa acgaagcaag
    27001 catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat tggcaggatg
    27061 gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata ataataaaaa
    27121 aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag cgcaacatct
    27181 gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca tagcgccccg
    27241 gcggcatggc gcgtaatttg tcccgccagg cgaggtttcg ctgatgcatc agcacatcgt
    27301 acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcggc ggtgcattca
    27361 gccaccagct catcatttgt tgcagcaacc gtgcgttggt gtgccagtgg gtcagcgtat
    27421 cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggcaatc tggttttcag
    27481 ccccttccag ttcaatcacg ggtttatgtt gttgcttagc cgcctgcaat agctggtaat
    27541 cgataccgta ttctgcccgc agccccagtt tttgcgcctg cgtcgcctga agaaccatcg
    27601 cgatttgcca cagcggttgg gtagaaaaga gcgacggaga aatgcccatc tcctggctaa
    27661 tgtgctgcag gttttggagt tggtcctcgc taatgcgctc ttccagggcc tcgcaggcag
    27721 gcaaattggc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc agcgcatcgg
    27781 cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg ctacccatat
    27841 gaatactgcc gatcagatga aaatggcgat tgccgggaag agagatatcg atagcaggcc
    27901 aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac aacagatcca
    27961 tacgacctcc ctttgtgaaa tatcatgcta gcgcgcgggg agggatggcg caaatggagt
    28021 tgattgttgc acttgtgcac agtgcttgtg tgcattacaa tgattaaata atggacaagg
    28081 agatgagaga ggaatggctc aaaagaaaaa tataaggagt tttcgtgatg cctggttggc
    28141 tgattttttt gtccattcaa cgccacatcg aaagatccct gctgaaatcc ataccacgtt
    28201 atcacgaaag ttggacatca ttaatgctgc cacttctcat cgggatttac gctcccctcc
    28261 ggggaaccga tacgaagagt tatccgggaa attacaggaa tactcctcca ttagagtgaa
    28321 taaacagtac cgactgattt ttaaatgggt taatggcaag gccgaagaat tgtttttaga
    28381 cccacacaac tactaaaaca tagctgtcgt attgacgaga aaggtaaaac tatgaaacag
    28441 gcaacaagaa aaccgacgac acccggagat attcttctct atgaatatct ggaaccgctc
    28501 gatttgaaaa tcaatgagtt agcagagttg ctgcatgttc atcgtaatag cgtcagtgca
    28561 ctgatcaata acaatcgtaa actcaccact gagatggcat tccgtctggc gaaagttttt
    28621 gataccacag tcgatttttg gctaaacctc caggcggcag ttgatctttg ggaagttgaa
    28681 aacaacatgc gtacccagga agaattggga cggattgaaa cagtggctga atatttggca
    28741 cgccgtgaag agcgtgcaaa aaaggtcgcg tagaataatt tccctataac ggatgcgcca
    28801 ccctaaagca gcgcatccgc aatgatgtac ttattccttc ggtttaaacc gcagcaatcg
    28861 gttggcgtta ctcactacgg tgatcgacga gagcgccatt gccgctccgg caaccaccgg
    28921 gttaagcagt gttccagtga acggccacaa aataccggcg gcgaccggaa taccaatgct
    28981 gttgtagata aacgcaccga gcagattctg cttcatgttg cgcagcgttg cgcgggaaat
    29041 tgcaagcgca tccgcaacac ccatcaggct atggcgcatc agggtaatcg ccgcggtttc
    29101 aatggcaaca tcactgccgc cacccatcgc aataccgaca tccgcctgag ccagcgctgg
    29161 cgcgtcgtta atgccgtcgc ctaccattgc cacctgacgt ccttcacttt gcagacgttt
    29221 gatcgcttcg gctttaccgt ctggcagcac gccggctatc acctcatcaa tccctgcttc
    29281 tttggcgatc gcattggcgg tggtcgggtt atccccggtt agcatcacca ggcgatatcc
    29341 cgctttatgc aggcgttgca gcgccgccac gctatcacta cgcaacggat cgcgtacagc
    29401 cagcagggct accgctttcc cgtcaaccgc cagcagcaca ggcgttgccc cttgcgatgc
    29461 ctgagctgaa atatctgctt caattgcttt ggtatcgacc tgttggtcat ttaacagcgc
    29521 ctgattgccc agcaataacg catgaccttc agcttcaccg ctcacgccca gcccgcgcaa
    29581 tgtgcggaaa ccgttgacct gcggtagctg catatcacct gctttatcga ggatcgcccg
    29641 tgccagaggg tggctggaac cttgctccag cgccgccgcc agacgcaatg cttgcgcttc
    29701 atcaatatca gcaaatgttt ttactgcgac aacctgcggc ttcccttcgg tcagcgtccc
    29761 ggttttatcg aacactacgg tgtcgagcgt actggcgcgt tgcagcgcgt cggcgtcccg
    29821 taccagcacg ccaaactcag ccgcccgccc gacgcccgaa ataatcgaca tcggcgtcgc
    29881 cagccccagc gcacacggac aggcaataat cagcaccgtg gtggcaatca ccagggtata
    29941 gacaatctgc ggtgccggac caaagaaata ccagattgcc gcactgatca gcgcaatcac
    30001 caccactacc ggcacaaata cggctgagat tttatccgcc agctgaccga tttctggctt
    30061 actgctctgg gcctggcgca ccatacgaat aatccgcgac agcgtagtat ggctgccaac
    30121 cgcactggcg cgaaacagca cgctaccgtc ctgcaccact gtcccggcat ggacgctatc
    30181 gccttcgcct ttttgctgcg ggataggttc gcccgtcagc atcgcttcat ccagccatgc
    30241 ttcgccctgg gtaatctctc catctaccgg tacgcgatcg ccggtcgtca ggcgcagcaa
    30301 catacctggc tgcacttccg ccagtggcac gcttttttca ccttcgtcag taaccaggcg
    30361 cgccgtcggc ggagttaaat cgagcaattt ctccagcgcc tttgaagaac gctgacgtgc
    30421 gcgcgcttcc agcatatgac cgagattaat cagaccgata atcatcgcac tggcttcata
    30481 gtaaagatgc cgcgcttcca tcgggaacca ctgcggccac aggttgacgc tcatcgaata
    30541 gagccacgcc acacccgttc ccagcgccac cagcgtatcc atcgtcgccg caccgttcag
    30601 caggcttttc catgcgctgc ggtaaaaatg cccgccggcg aaaaccatta ccgccagggt
    30661 tatcaggccg ataactaacc acaggctgcg gttgtcagcg gtgaccatca tgttatcgcc
    30721 gatcatcccc cagaccatca ccgggatacc caccgccagc gcgacaattg cctgccagcg
    30781 gaagcgcttc atcgtagcga cggcggtttc ttgctggcgc tcgcggcgtt tagcgtcatc
    30841 ttcaatcgct tccgcgccgt agcccgcttt ttccaccgcc tgcactaaat cttgtgggga
    30901 ggcactgccc atcaccagcg cagtacgctc cgccaggttt acccgtgcct gagtgacgcc
    30961 cggtacgctt tgcaacgcat tttgtacgcg ggtgacacag ctggcgcagc tcatgccgct
    31021 cagcagcaac tgctggctgt catcgtcatc ggcggtcgct gccggaagcg cctcagaaac
    31081 cgctgtcagt gcttccgacg ggattgatga ctccgccagc ggtttagcct ttgggtggct
    31141 tacagatgcg tcataacccg cttgtttgat ggtttcgatt agctgttctg cactggcagt
    31201 cccggtaacg tgcgcttcag tgatagacac atccgcctgt tcaacatccg ggcgctgttc
    31261 gagactttct ttcacgcgtt taacgcagtg accgcaggac aggccgtcca gggtcaggtc
    31321 gatagtttgt gacataaaac actcctttaa gacagttttg actggctgtg ataaaggtta
    31381 aaccttccag caaggggaag gtcaagaaat taataaacca ggcgggtaaa agtccgtaaa
    31441 gattaaaaaa tcggctcgat ttgcatcagg attagacatt tatctctttg ttttcctgta
    31501 gttaagttgc gggtgctaag ttaaatcagg atgcatgaaa atcggcaccg gggtgaggaa
    31561 ttacctcccg catctataaa aaggagttaa caaaagatgt tagatgcaaa caaattacag
    31621 caggcagtgg atcaggctta cacccaattt cactcactta acggcggaca aaatgccgat
    31681 tacattccct ttctggcgaa tgtccctggt caactggcgg cagtggctat cgtgaccagc
    31741 gatggcaacg tctatagtgc gggtgacagt gattaccgct ttgcgctgga atccatctcg
    31801 aaagtctgta cgttagccct tgcgttagaa gatgtcggcc cgcaggcggt acaggacaaa
    31861 gttggcgctg acccgaccgg attgcccttt aactcagtta tcgccttaga gttgcatggc
    31921 ggcaaaccgc tttcgccact ggtaaatgct ggcgctattg ccaccaccag cctgattaac
    31981 gctgaaaata ctgaacaacg ctggcagcga attttacata tccaacaaca actggctggt
    32041 gagcaggtag cgctctctga tgaagtcaac cagtcggaac aaacaaccaa cttccataac
    32101 cgggccattg cctggctgct gtactcagcc ggatatctct attgtgatgc aatggaagcc
    32161 tgtgacgtgt acacccgtca gtgctccacg ctcatcaata ctattgaact ggcaacgctt
    32221 ggcgcgacgc tggcggcggg tggtgtgaat ccgctgacgc ataaacgcgt tcttcaggcc
    32281 gacaatgtgc cgtacattct ggccgaaatg atgatggaag ggctgtatgg tcgctccggt
    32341 gactgggcgt atcgcgttgg tttaccgggc aaaagcggtg taggtggcgg tattctggcg
    32401 gtcgttcctg gcgtgatggg aattgccgcg ttttcaccac cactggacga agaaggcaac
    32461 agtgttcgcg gtcaaaaaat ggtggcatcg gtcgctaagc aactcggcta taacgtgttt
    32521 aagggctgat catgatgaac acggaaggta ataacggtaa caaacccctc ggtctatgga
    32581 acgtcgtttc catcggcatt ggggcaatgg tgggggcggg gatcttcgcg ctgctggggc
    32641 aggctgcgtt gctaatggaa gcctcgacct gggtcgcctt tgcttttggc ggtattgtgg
    32701 cgatgttttc cggttatgcc tatgcgcgtc tgggggcgag ctatcccagc aatggcggta
    32761 ttatcgactt ctttcgtcgc ggattaggca acggcgtttt ttcgctggcg ctctcgttat
    32821 tgtacctgtt gacgctggcg gtgagtatcg ccatggtcgc ccgtgctttt ggcgcttatg
    32881 ccgtgcagtt tttgcatgaa ggcagtcagg aggagcacct tattttgctc tacgcattgg
    32941 ggatcattgc ggtgatgacg cttttcaact ccttaagcaa ccatgcggta gggcggctgg
    33001 aagtgatcct cgtcggcatt aaaatgatga tcctgttatt gctgattatt gccggtgtct
    33061 ggtcgctgca accggcgcat atttccgttt ctgcgccccc cagctccggg gcgttcttct
    33121 cctgtatcgg gataaccttc cttgcctatg cgggctttgg catgatggcg aacgcggcgg
    33181 ataaagtgaa agatccggag gtcataatgc cacgggcgtt tctggtggcg attggcgtta
    33241 ccacgttgct ttatatctcg ctggcgctgg ttttgcttag cgatgtatcg gcattagagt
    33301 tagaaaaata tgccgatacc gccgtagcgc aggctgcttc tccgctcctc gggcatgtgg
    33361 ggtatgtgat cgtcgtcatc ggcgctttac tggcgacggc ttcagccatc aacgcaaacc
    33421 tgttcgccgt gtttaacatc atggacaaca tgggtagcga acgcgaactg ccgaagctaa
    33481 tgaataaacc tctgtggcgg cagagtacct ggggtaacat catcgtcgtg gtgttgatta
    33541 tgctgatgac ggcggcgctg aatttaggct cactcgccag cgttgccagt gccacctttt
    33601 tgatctgcta tctggcggtg tttgtggtgg cgatccgcct gcgtcatgat attcacgcct
    33661 cgttgccgat tcttatcgtt ggtacgttgg ttatgttgtt ggtgatcgtt ggctttatct
    33721 acagtctctg gtcccagggc agccgtgcgc tgatatggat tattggcgca ctcttactca
    33781 gccttattgt ggcaatggtc atgaagcgca ataaaaccgt ataacatctc tctgtgcgca
    33841 gtacctcctg tattattgtg gtggcggtcg atattcgcac tggcaaaaaa acgtgcttga
    33901 atatctgttg aaacccttta acaaagcaca ggaggcgttg cgcgaacgat gaacattagc
    33961 gatgtagcaa aaattaccgg cctgaccagc aaagccattc gcttctatga agagaagggg
    34021 ctggtgacgc cgccgatgcg cagcgaaaac ggttatcgca cctacacgca gcagcatctc
    34081 aacgaactga ccttactgcg ccaggcacgc caggtgggct ttaacctgga agagagcggc
    34141 gaactggtga atctgtttaa cgatccgcag cgtcacagcg ccgacgtcaa acgtcgtacg
    34201 ctggagaaag tagcggagat cgaacagcat atcgaagaac tgcaatccat gcgtaaccag
    34261 ctgctggcgc tggcgaatgc ctgccccggt gatgacagcg ccgactgccc gattatcgaa
    34321 aatctctccg gctgctgtca tcatcgggcg gggtgattaa ggcttgcgaa aatagtcgtc
    34381 cattattttc gttagctccg gacgttcccc gtcatcattg atggcaaaat ccagaaaaaa
    34441 agcatctgct tgcggcgttc ggttttgcgc catcaaccgg aaggtgaggg ggcctgacca
    34501 ggtttcggat gggctgatgt gcttatcagg gccgaaactc tcccatgcgc tgggcggtaa
    34561 tgttttgcac gcggtgagtt ttggtatgtg gtattgcagc tctgttttgt gcagacattc
    34621 cccgccagga aataccaggg tttgttgctc atcattaaaa tgggttgccg tgctgagcac
    34681 cagtgttttg agcggtcgcc cttccagcgc ggcggcagta gccttatggt gtccgtcgag
    34741 tagaacgcag agataggaat cagggagata ccaggccaga ccacgggcaa ttccagtttt
    34801 cgagagcaga caaaaaccgg gcggctccct gttcatcctc cgcaaaaccg ctgtagacaa
    34861 attccgcaca ggtagggcat ccagcctcgg accagaaacg tattaacggg acatcctgcc
    34921 agcagagcga atggataaga ccgtgcgagt gcggatcgta aatcacacat tgacgcagaa
    34981 atcctttccc ggacttcacc gaaaaaatga cgcccgcagc ggtatttttc agcagacgta
    35041 cttcaggttt tcgaaagggg gaaacatggc tactccttag ccgaacattc aattaacact
    35101 atgtgggggg ctatatgccg gatgcggcgt aaacgcctta tccggcatgg gttttggtac
    35161 agatttccag gtgtgataag acgcgtcagg cgatgaccgc acggataatc agcgttattc
    35221 cttctatcgc aatcacctca acatgcgtac cagcgccgag atcctcgctg gcgctgacag
    35281 gccatgaact gtcaccgacg cgcatatgac cgcgcccgtt gaccagcgga gattccagaa
    35341 caaaacgtcg gccaatcaac tgctgcccgc gctggtttaa atgactgtcg ctgtgctttt
    35401 gttcgcgcac ccgccgcgac aaccatttcc accacagcca ggcggcgagc agcgtcagga
    35461 cggcaaacat taccccttgc cactcccaac ccagcggcag caaccagacc accagaccgg
    35521 taatcaccgc tgccacgccg ctccacaaca gataaccatt gccgcccaac atctcggctg
    35581 ccagcagcaa accgccgaga cttagccaga aaatatgtgg atgaacgacc attaactcca
    35641 tcatggctga gtccgtttgt tggcgctgtc tttcaccagc tcagcaatcc cggcaatcga
    35701 ccccatcagg ctgctggcct ctaatggcat catcaccact ttgctgttac tggacgaacc
    35761 gatctgctgt aacgcttcgg tgtatttctg tgcgacgaag tagttcaccg cctgaatatc
    35821 accggaggcg atggcttcag acaccatttt ggtggcgcgg gcttctgctt cagcggaacg
    35881 ttcacgcgct tcagcctgta aaaacgccga ctgacgttcg ccttccgctt tcaggatttg
    35941 tgactgtttt tcaccttcgg ctttgaggat ttccgcctga cggatccctt ccgcttcaag
    36001 gatgtaagcg cgtttggtac gttccgcttt catctgcgcg ttcattgaag agataagctc
    36061 tgcgggcggg cgcacatcac gaatttcgat acgggtgact ttaatccccc acgggttggt
    36121 ggcctcatcg acaatatgca gcagacgcga gttaatgctg tcgcgctgag agagcatttc
    36181 atcaagttcc attgaaccca gcacggtacg gatgttagtc atggtcaggt tgatgatcgc
    36241 cagctccaga ttgctgactt cataagccgc gcgcggcgcg tcaatcacct gaataaagca
    36301 cacagcgtcg atggtaacgt tggcgttatc tttcgagata acttcctggg aagggatgtc
    36361 gagcacttgc tccatcatat tgatcttgcg accaatgcga tccataaacg gcaccaccag
    36421 gcttagacct ggctgtaacg ttttggtata gcgaccaaag cgttctaccg tccattgata
    36481 gccctgcggc acgattttga cgcccgcgcc gacaatgacc agcgcgacaa aaatgagaat
    36541 cgggataaag ataagcatcg gaaaaacctc ctgttgtacc gtccataatc agcaaaattg
    36601 ctgcttgatt aaacaaatta tacctgatta ctgaaagaga gttccccctt attcctgcga
    36661 aggataaact gtttttagta aaaatcagaa aaagggaaca gcgatgcagg aaaatagtcc
    36721 tttgcttcag ctacaaaacg taggatatct ggcgggtgat acgaagattc ttaataacat
    36781 caatttttcg ctgcgtgctg gcgaatttaa gttaattacc ggtccttctg gttgtggcaa
    36841 aagtacactg ctaaaaatag ttgcttcatt gatcagccca accagcggaa cgttactgtt
    36901 tgaaggtgag gatgtcagca cactaaagcc agaaatctac cgtcagcaag tctcttactg
    36961 cgcccagacg ccgacgctgt ttggcgatac ggtatacgat aacctgattt ttccctggca
    37021 gatccgtaac cagcagcctg acccggccat ttttctcgat tttcttgaac gcttcgcttt
    37081 accggacagc attttgacga agaatatcgc cgagctatct ggtggtgaaa aacaacgcat
    37141 ctcattgatt cgtaacctgc aatttatgcc gaaggtttta ttgctcgacg aaatcaccag
    37201 tgcgctggat gaaagtaata aacataacgt taatgagatg atccaccgtt atgtacgcga
    37261 gcaaaatatt gctgtgctgt gggtgacaca cgataaagac gaaattaatc atgcggataa
    37321 agtgattaca ctgcaaccgc atgctggaga aatgcaggaa gcacgctatg aactcgcata
    37381 atattactaa cgaatcatta gcactggcat taatgctggt ggtggtggca atcttaatta
    37441 gccataaaga aaaactggcg ctggagaaag atattctctg gagcgtcggg cgcgcgataa
    37501 tccagctgat tattgtcggc tatgtgctga agtatatttt cagcgtggat gatgccagcc
    37561 tgacattatt gatggtgtta tttatctgct ttaatgcggc gtggaacgcg caaaaacgca
    37621 gtaaatatat tgctaaagct tttatctcat cgtttattgc cattacggtc ggggcgggaa
    37681 ttaccctggc ggtactgatc ctctctgggt cgattgaatt tatcccgatg caggtgatcc
    37741 ctatcgccgg gatgatagcc ggtaacgcca tggtagcggt ggggttgtgt tacaacaatt
    37801 tagggcaacg ggtcattagc gaacagcaac agattcagga gaaactgagt cttggcgcga
    37861 cgccgaaaca ggcttcagcg atattgattc gcgacagtat tcgcgcggct ttaattccga
    37921 cggtcgattc agcaaaaacg gtaggcttag tgagtttacc aggaatgatg tccgggctga
    37981 tatttgccgg gattgatccg gtgaaggcga ttaaatatca gattatggtg acctttatgc
    38041 tgctctcaac cgccagcttg tcgaccatta ttgcctgcta tttaacctat cgtaagtttt
    38101 acaattcgcg ccaccaattg gtggtgacgc aattgaagaa gaaatgatgc gatgccggat
    38161 gcgtcttatc agacctacat ggcacctgca atgtattgaa ttagcacggt ttggtaggcc
    38221 ggataaggcg ttcacgccgc atccggcggc tatgcgcgga tgcctgatgc gacgctggcg
    38281 cgtcttatca ggcctacaaa ccagcacagc atcgcatttc aacggaaaat caatacaaca
    38341 acgcatacag ctggcggcga tacttcgacg ccagtgcatc acccgtaccc agcgcagcga
    38401 ggatctcctg gaatgtttta cgtgtctgac catctgcggc ggtgagatct ttacgcagat
    38461 gcccaaacag taactccagc gcctcttcat tgcgcccaac ctggtgcagt tgcagtgcca
    38521 gttgcgtcgc cagtgcggca tcttctggat tctccgccac ctgctgttgc aactgttgaa
    38581 tttccggcgt atcagccgcc tgcttcagca gttcgatttg cgccaccagc ccctgatagc
    38641 gggtgtcctg atcctgcaac ggaatggttt tcagcacggc ttctgcatct tcagaacggt
    38701 tcagcgcaat cagcgtttct gccaacagca ggccgatttc cccgttctga ttcgacaact
    38761 gccaggcgtc tttcagcaat ggcagggcgt cggtgtaatt gccttcctgc atcagttgca
    38821 tcgcctgctg cgctttcagc tcttcttcac gcggcagcac tttatccagc aggacgcgga
    38881 tcgcctcttc cggttgcggc ccctggaagc cgtctactgg ttgcccgttc tggaacagat
    38941 atacggtcgg aatcgcgcgc agaccaaact gggcggcaat catctgctcc gcgtcgcagt
    39001 ccagcttcgc cagaataaat tgcccgttgt actgcgccgc gaggctttcc agaattgggg
    39061 ttaactgcaa acagtgctgg ctacgttcag accaaaaata gaacagcacc ggagtggtca
    39121 tcgactgttc aagaacctgt tgcaggttag attcgttaat gttgacaata ttttctacgg
    39181 acatggagtc gctctctgtt gtcgatattt tctttgacat gggggcttaa gcgcgcgctt
    39241 caactcaccc ctgcaatatt ttgtccatca cgcgccccgg cagcaggcgc ttaagcacca
    39301 ttaccgccct ggtcaccagc gtcaccggat agcgcatctt cggcttctcg ctaataaaag
    39361 catggcgcac tttgccgacc accgcttccg gtcccaacgt aaagcgggcg gcgatgccag
    39421 gattttcgac tggtttatca ctttgcgtct ggttgacgtt gtcggtgaag cgagtacgaa
    39481 tgggaccagg ttcgatcagg ctgactttaa ttccgctgtg gcgcagctcc atgcgcagtg
    39541 cgtctgacca cgcctccagc gcatatttac tggccgcgta agcgccacga cccggtgtgg
    39601 agattaatcc catcaccgat gaggtcatca caatacgccc ttcacggtgc ggcaacatcg
    39661 cgggtaacag gcgcatggtg agctggtgtg cgccgaaaaa gttagcggaa aactgctgtt
    39721 ccatctgcgc acggctgatg gtggaaaggg ggccatacat gccgaatccg gcattgttaa
    39781 agatcccata cagacaatta tcggtcaggg cgatcacttc gtcggctgcg cgatcaacac
    39841 tttctggcga atccagatcg atcaacacgc cggtaaatcc catgctgttc atgcgctcaa
    39901 catcatccgg tttccggcaa cctgccagca catgaaaacc ctggcgtttt aattcgagtg
    39961 cgctttccag gccaatacca ctggaacatc cggtaattaa gaccgatttt tgcataactt
    40021 tacctgtcag gatctccgtt gctttatgag tcatgattta ctaaaggctg caactgcttc
    40081 gccatccagt cggcaataaa cggctgggcg tcgcggttgg gatgaatacc gtcatcctgc
    40141 atccattgtg gcttgaggta gacctcttcc ataaaaaagg gcagcagcgg aacatcaaac
    40201 tctttggcga gtttggggta aatggcgcta aaggcttcat tataacggcg accatagttt
    40261 gcaggcagac gtatttgcat taacaatggt tcagcgttgg cggctttgac atcctgcaaa
    40321 atctggcgca gcgtttgctc ggtttgctgt ggctgaaaac cacgcaaacc gtcattgccg
    40381 cccagttcaa ccagcaccca gcgcggctga tgctgtttca gcagagccgg aaggcgcgcc
    40441 agcccttgtt gcgaggtgtc accgctgatg ctggcattga ctaccgacgt tttactctgc
    40501 cacttatcat tcaacaaggc aggccaggcc gcgctggcag acattcgata cccggcgctc
    40561 aggctatcac ccagaatcaa taacgtgtcc gctgcggcgg cacggaaggt taacaggacc
    40621 aggaacagga agggcaaatg ccagcggaaa acattgttga agttcatcat cttaagaagt
    40681 ccgtcggtca gggggagcat gaactctcca tcctcaccgg agttgagctg gttgtcaaac
    40741 gtggcgagac catcgcactg gtgggcgagt cgggatcggg taagtcaacc ttgctggcga
    40801 tcctcgccgg gctggatgac ggcagcagtg gcgaagtgag tctggtggga caaccgctac
    40861 ataatatgga cgaagaagcg cgggcaaagt tgcgcgcgaa gcacgtaggc tttgtttttc
    40921 agtcatttat gttaattcct acccttaacg cgctggaaaa cgtcgagctt ccggcgctgc
    40981 tgcgcggtga gagtagcgca gaaagtcgta acggggcgaa agcgttgctc gaacagttag
    41041 ggctgggtaa acgtcttgat catcttccgg cacagctttc tggcggcgaa cagcaacgag
    41101 tggcgctggc gcgagccttt aatggtcgac ctgatgtgct gtttgccgac gaacccaccg
    41161 gtaaccttga ccgccagacg ggcgataaaa ttgccgatct gcttttttcc ctcaaccgtg
    41221 agcatggcac cacgttgatt atggtgaccc acgacctgca actggcggca cgctgtgacc
    41281 gctgcttacg gctggtgaac gggcagttgc aggaggaagc atgattgcac gttggttctg
    41341 gcgcgaatgg cgctcgccgt cgctattaat tgtctggctg gcgcttagcc tggcggtggc
    41401 ctgcgtgctg gcgctgggca atatcagcga tcgcatggag aagggcttaa gccagcaaag
    41461 ccgtgagttt atggcgggcg atcgggcgtt gcgcagttcg cgcgaagtgc cgcaagcatg
    41521 gctggaggaa gcgcaaaagc gcggcctgaa agttggcaag cagctgactt tcgccaccat
    41581 gacctttgca ggcgacacgc cgcaactggc gaacgtcaaa gcggtggatg atatctaccc
    41641 gatgtatggc gatctgcaaa ctaatccccc tggcctgaaa ccgcaggcgg gcagcgtatt
    41701 gctggcccca cgcctgatgg cactgcttaa cctgaaaacg ggcgatacca ttgacgtggg
    41761 cgatgccacc ttgcggattg ccggagaagt gattcaggaa ccggattccg gttttaatcc
    41821 tttccagata gccccacgcc tgatgatgaa tctggcggat gttgataaaa ccggagccgt
    41881 gcagccgggg agtcgggtca cctggcgtta taaattcggc ggcagcgaga accagctcga
    41941 cggctatgag aaatggttgt taccccagct taaacccgaa caacgctggt acggtctgga
    42001 acaggacgaa ggcgcgctgg ggcgatcgat agaacgttcg caacagttcc tgctgctttc
    42061 ggcgcttctg accttgctgc tggcggtggc ggcggtcgcg gtggcgatga atcattactg
    42121 tcgcagtcgc tacgatctgg tggcgatcct caagacgctg ggggcagggc gagcgcaact
    42181 gcgtaagtta atcgtcggtc agtggttgat ggtgctggta ctttcagccg ttaccggtgg
    42241 ggcgataggc ctgttgttcg aaaacgtgtt gatggtgctg ctcaagcccg ttctgcctgc
    42301 tgcactaccg ccagccagcc tctggccgtg gctgtgggcg ctcggcacca tgacggttat
    42361 ttcgctgctg gtggggctac gaccataccg cctgttgttg gcaacgcagc ctttacgcgt
    42421 attacgtaat gatgtggtag cgaacgtctg gccgctgaag ttttatctgc cgattgtcac
    42481 cgtggtggtt gtgctactgc tcgccgggct gatgggcggc agtatgctgc tctgggcggt
    42541 gctggcgggc gcggtagtac tggctttgct gtgcggtgtc ctgggctgga tgctgctgaa
    42601 tgtacttcgc cgcatgacgc tgaaatcgct acctctgcgc ctggcggtta gccgcctgtt
    42661 acgtcagccg tgggcgacgt tgagccagct ttcggcattt tcgctctcct ttatgctgct
    42721 ggcactgttg ctggtgttgc gtggcgatct gctcgaccgc tggcaacagc agctaccgcc
    42781 agaaagtcct aactactttt taatcaacat cgctacggaa caggttacgc cgctgaaagc
    42841 gtttctcgcg gaacatcaga tagtcccgga atcgttttat ccggtggtgc gggcgcggtt
    42901 gacggcgatt aacgacaagc cgacagaagg caatggagat gaggcgctca accgcgagct
    42961 gaatctcacc tggcaaaata cgcggcctga tcataacccg atcgtcgccg gtaactggcc
    43021 gccaaaagcc ggtgaagtgt cgatggaaga ggggctggcg aaacgcttaa acgttgccct
    43081 cggcgatacc gtgactttta tgggcgatac ccaggagttc cgcgctaaag tgaccagcct
    43141 gcgcaaagtg gactgggaaa gtctgcggcc taatttctat tttattttcc cggaaggggc
    43201 attagacggg caaccgcaga gctggcttac cagtttccgc tgggagaatg gcaacggcat
    43261 gttgacgcaa ctcaaccgcc agttcccgac cattagcctg ttagacattg gcgcgatttt
    43321 aaaacaggtc ggtcaggtgc tggagcaggt aagtcgggcg ctggaagtga tggtggtgct
    43381 ggtcaccgcc tgcggtatgt tgctgttgct ggcacaggtg caggtgggaa tgcgtcagcg
    43441 tcatcaggag ctggtggtgt ggcgcactct cggtgcgggc aaaaaactgc tgcgcacgac
    43501 attgtggtgt gagttcgcca tgctcgggtt tgtatccggc ctggtggccg caattggtgc
    43561 ggaaacggca ctggcggtac tgcaatcgaa agtgtttgat ttcccgtggg agccagactg
    43621 gcgattgtgg attgtgctgc cgtgcagcgg tgcgctactg ctgtcgcttt gcggcggctg
    43681 gctgggtgcg cgactgctta agggtaaggc gctattcagg cagtttgtgg ggtgatgtga
    43741 taaagccgtt tatttctacc tggtagataa acggctttat tattagtgca tcacgtaatt
    43801 aaaaa
//
LOCUS       DACADF010000020        43666 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.020, whole genome shotgun sequence.
ACCESSION   DACADF010000020 DACADF010000000
VERSION     DACADF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.020"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            204..1298
                     /gene="gcvT"
                     /locus_tag="ISB30_001086"
     CDS             204..1298
                     /gene="gcvT"
                     /locus_tag="ISB30_001086"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAP1988741.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1322..1711
                     /gene="gcvH"
                     /locus_tag="ISB30_001087"
     CDS             1322..1711
                     /gene="gcvH"
                     /locus_tag="ISB30_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAP1988742.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1830..4703
                     /gene="gcvP"
                     /locus_tag="ISB30_001088"
     CDS             1830..4703
                     /gene="gcvP"
                     /locus_tag="ISB30_001088"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAP1988743.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGALLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLAEWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLIRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4869..6302)
                     /locus_tag="ISB30_001089"
     CDS             complement(4869..6302)
                     /locus_tag="ISB30_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP1988744.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQKSMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            6347..6658
                     /locus_tag="ISB30_001090"
     CDS             6347..6658
                     /locus_tag="ISB30_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP1988745.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            6822..7481
                     /locus_tag="ISB30_001091"
     CDS             6822..7481
                     /locus_tag="ISB30_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP1988746.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     V"
     gene            complement(7558..8538)
                     /gene="ygfZ"
                     /locus_tag="ISB30_001092"
     CDS             complement(7558..8538)
                     /gene="ygfZ"
                     /locus_tag="ISB30_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAP1988747.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARTALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            8781..9047
                     /gene="sdhE"
                     /locus_tag="ISB30_001093"
     CDS             8781..9047
                     /gene="sdhE"
                     /locus_tag="ISB30_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAP1988748.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9028..9435
                     /locus_tag="ISB30_001094"
     CDS             9028..9435
                     /locus_tag="ISB30_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988749.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9475..9996)
                     /gene="fldB"
                     /locus_tag="ISB30_001095"
     CDS             complement(9475..9996)
                     /gene="fldB"
                     /locus_tag="ISB30_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAP1988750.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10108..11004
                     /gene="xerD"
                     /locus_tag="ISB30_001096"
     CDS             10108..11004
                     /gene="xerD"
                     /locus_tag="ISB30_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP1988751.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11029..11739
                     /gene="dsbC"
                     /locus_tag="ISB30_001097"
     CDS             11029..11739
                     /gene="dsbC"
                     /locus_tag="ISB30_001097"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAP1988752.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            11745..13478
                     /gene="recJ"
                     /locus_tag="ISB30_001098"
     CDS             11745..13478
                     /gene="recJ"
                     /locus_tag="ISB30_001098"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP1988753.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEEKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            13569..14667
                     /gene="prfB"
                     /locus_tag="ISB30_001099"
     CDS             join(13569..13643,13645..14667)
                     /gene="prfB"
                     /locus_tag="ISB30_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP1988754.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14677..16194
                     /gene="lysS"
                     /locus_tag="ISB30_001100"
     CDS             14677..16194
                     /gene="lysS"
                     /locus_tag="ISB30_001100"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP1988755.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16237..16785)
                     /gene="idi"
                     /locus_tag="ISB30_001101"
     CDS             complement(16237..16785)
                     /gene="idi"
                     /locus_tag="ISB30_001101"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP1988756.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEEAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLAAVLRGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            16840..16911
                     /locus_tag="ISB30_001102"
     CDS             16840..16911
                     /locus_tag="ISB30_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988757.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(16908..17033)
                     /locus_tag="ISB30_001103"
     CDS             complement(16908..17033)
                     /locus_tag="ISB30_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988758.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17035..18483)
                     /locus_tag="ISB30_001104"
     CDS             complement(17035..18483)
                     /locus_tag="ISB30_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP1988759.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSQDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            18919..20838
                     /gene="ygfT"
                     /locus_tag="ISB30_001105"
     CDS             18919..20838
                     /gene="ygfT"
                     /locus_tag="ISB30_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HAP1988760.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            20838..21326
                     /locus_tag="ISB30_001106"
     CDS             20838..21326
                     /locus_tag="ISB30_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP1988761.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASSEN
                     PL"
     gene            complement(21362..22729)
                     /locus_tag="ISB30_001107"
     CDS             complement(21362..22729)
                     /locus_tag="ISB30_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP1988762.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22765..24081)
                     /gene="guaD"
                     /locus_tag="ISB30_001108"
     CDS             complement(22765..24081)
                     /gene="guaD"
                     /locus_tag="ISB30_001108"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HAP1988763.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24099..25499)
                     /gene="xanQ"
                     /locus_tag="ISB30_001109"
     CDS             complement(24099..25499)
                     /gene="xanQ"
                     /locus_tag="ISB30_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HAP1988764.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGISYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(25664..28534)
                     /locus_tag="ISB30_001110"
     CDS             complement(25664..28534)
                     /locus_tag="ISB30_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HAP1988765.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQILGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(28531..29310)
                     /locus_tag="ISB30_001111"
     CDS             complement(28531..29310)
                     /locus_tag="ISB30_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HAP1988766.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWIDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(29361..30689)
                     /gene="ssnA"
                     /locus_tag="ISB30_001112"
     CDS             complement(29361..30689)
                     /gene="ssnA"
                     /locus_tag="ISB30_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HAP1988767.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIDGSLSTLRNAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFTRQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     STGRGLHIHVAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIVLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(30692..33790)
                     /gene="ygfK"
                     /locus_tag="ISB30_001113"
     CDS             complement(30692..33790)
                     /gene="ygfK"
                     /locus_tag="ISB30_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HAP1988768.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKSKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWELNHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDTILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(34112..34690)
                     /gene="mocA"
                     /locus_tag="ISB30_001114"
     CDS             complement(34112..34690)
                     /gene="mocA"
                     /locus_tag="ISB30_001114"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HAP1988769.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            34794..35564
                     /gene="yqeC"
                     /locus_tag="ISB30_001115"
     CDS             34794..35564
                     /gene="yqeC"
                     /locus_tag="ISB30_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HAP1988770.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHCWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            35612..37237
                     /locus_tag="ISB30_001116"
     CDS             35612..37237
                     /locus_tag="ISB30_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HAP1988771.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLHQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(37278..38210)
                     /gene="arcC"
                     /locus_tag="ISB30_001117"
     CDS             complement(37278..38210)
                     /gene="arcC"
                     /locus_tag="ISB30_001117"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP1988772.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(38258..39643)
                     /locus_tag="ISB30_001118"
     CDS             complement(38258..39643)
                     /locus_tag="ISB30_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HAP1988773.1"
                     /translation="MRVLIKNGIVVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(39696..40907)
                     /locus_tag="ISB30_001119"
     CDS             complement(39696..40907)
                     /locus_tag="ISB30_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HAP1988774.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(40965..42161)
                     /gene="dpaL"
                     /locus_tag="ISB30_001120"
     CDS             complement(40965..42161)
                     /gene="dpaL"
                     /locus_tag="ISB30_001120"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HAP1988775.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(42219..43406)
                     /gene="ygeW"
                     /locus_tag="ISB30_001121"
     CDS             complement(42219..43406)
                     /gene="ygeW"
                     /locus_tag="ISB30_001121"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HAP1988776.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT        10591 a        10957 c        11485 g        10633 t
ORIGIN      
        1 attctgttac gattttggcg ttttttaacc ataagctaat gtgatgatca attttacctt
       61 atggttaaca gtctgtttcg gtggtaagtt caggcaaaag agaacgattg cgttggggac
      121 cgggagtggc tccgatgctg ggtttcgtgg tgataatttc gccatagaaa agttgtcagc
      181 cccgcttatt caatgaggac aagatggcac aacagactcc tttgtacgaa caacacacgc
      241 tttgcggcgc tcgcatggtg gatttccacg gctggatgat gccgctgcat tacggttcgc
      301 aaatcgacga acatcatgcg gtacgtaccg atgccggaat gtttgatgtg tcacatatga
      361 ccatcgtcga tcttcgcggc agccgcaccc gggagtttct acgttatctg ctggcgaacg
      421 atgtggcgaa gctcaccaaa agcggcaaag ccctttactc ggggatgttg aatgcctctg
      481 gcggtgtgat agatgatctc atcgtctact actttactga agatttcttc cgcctcgttg
      541 ttaactccgc cacccgcgaa aaagacctct cctggattac ccaacacgct gaacctttcg
      601 gcatcgaaat tactgttcgt gatgaccttt ccatgatcgc cgtacaaggg ccgaatgcgc
      661 aggcaaaagc tgccacactg tttaatgacg cccagcgtca ggcggtggaa gggatgaaac
      721 cgttctttgg cgtgcaggcg ggcgatctgt ttattgccac caccggttac accggtgaag
      781 cgggctacga aattgcgctg cccaatgaaa aagcggccga tttctggcgc gcgctggtgg
      841 aagctggcgt taagccatgt ggcctgggcg cgcgtgacac gctgcgtctg gaagcgggca
      901 tgaatttgta tagccaggag atggacgaaa ctatttctcc tttagccgcc aatatgggct
      961 ggactatcgc ctgggaaccg gcagatcgtg actttatcgg tcgtgaagct ctggaagcgc
     1021 agcgtgaaca tggcacagaa aaactggttg gtctggtgat gaccgaaaaa ggcgtgctgc
     1081 gtaatgaact gccggtacgt tttactgatg cgcagggcaa ccagcatgaa ggcattatca
     1141 ccagcggtac tttctcccca acgctgggtt atagcattgc gctggcgcgc gtgccggaag
     1201 gtattggtga aacggcgatt gtgcaaattc gcaaccgtga aatgccggtt aaagtgacga
     1261 aacctgtttt tgtgcgtaac ggcaaagccg tcgcgtgatt tacttttttg gagattgatt
     1321 gatgagcaac gtaccagcag aactgaaata cagcaaagaa cacgaatggt tgcgtaaaga
     1381 agccgacggc acttacaccg ttggcatcac cgaacacgct caggagctgt taggcgatat
     1441 ggtgtttgtt gacctgccgg aagtgggcgc aacggttagc gcgggcgatg actgcgcggt
     1501 tgccgaatcg gtaaaagcgg cgtcagatat ttatgcgcca gtaagcggtg aaatcgtggc
     1561 ggtaaacgat gcactgagcg attctccgga actggtcaac agcgaaccgt atgcaggcgg
     1621 ctggattttc aaaatcaaag ccagcgatga aagcgaactg gaatcactgc tggatgcaac
     1681 cgcatacgaa gcattgttag aagacgagta acggctttat tcctcttctg cgggagagga
     1741 tcagggtgag gaacatttac gcctcaccct cactctcttc gtaaggagag aggttcacaa
     1801 ttcactgcac gtttcaggaa ccatcgctca tgacacagac gttaagccag cttgaaaaca
     1861 gcggcgcttt tattgaacgc catatcggac cggacgccgc gcaacagcaa gaaatgctga
     1921 atgccgttgg cgcacaatcg ttaaacgcgc tgaccggcca gattgtgccg aaagatattc
     1981 agcttgcgac tccgccgcag gttggcgcac cggcgaccga atacgccgca ctggcagaac
     2041 tcaaggctat tgccagtcgc aataaacgct tcacgtctta catcggcatg ggttacaccg
     2101 ccgtgcagct accgccggtt atcctgcgta acatgctgga aaatccgggc tggtataccg
     2161 cgtatactcc gtatcaacct gaagtctccc agggccgcct tgaagcactg ctcaacttcc
     2221 agcaggtaac gctggattta accgggctgg atatggcctc tgcttctctt cttgacgaag
     2281 ccaccgctgc cgccgaagca atggcgatgg cgaaacgcgt cagcaaactg aaaaatgcca
     2341 accgcttctt tgttgcttct gacgttcatc cgcaaacgct ggatgtggtc cgtactcgtg
     2401 ccgaaacctt tggttttgaa gtgattgtcg atgatgcgca aaaagtgctc gaccatcagg
     2461 acgtcttcgg cgcgctgtta cagcaggtcg gcactactgg tgaaattcac gactacactg
     2521 cgcttattag cgaactgaaa tcacgcaaaa ttgtggtcag cgttgccgcc gatattatgg
     2581 cgctggtgct gttaactgcg ccaggtaaac agggcgcgga tattgttttt ggttcggcgc
     2641 aacgctttgg cgtaccgatg ggctacggtg gcccacacgc ggcattcttt gcggcgaaag
     2701 acgaatacaa acgctcaatg ccgggccgta ttatcggtgt atcgaaagat gcagctggca
     2761 acaccgctct gcgcatggcg atgcagactc gtgagcaaca tattcgtcgt gaaaaagcga
     2821 actccaacat ttgtacatcc caggtcttgc tggcaaacat tgccagtctg tatgccgttt
     2881 accacggtcc ggttggcctg aaacgtatcg ctaaccgcat ccaccgtctg accgatatcc
     2941 tggcggcggg cctgcaacaa aaaggtctga agctgcgcca tgcgcactat ttcgatactt
     3001 tgtgtgtgga agtggccgac aaagcgggcg tgctggcgcg tgccgaagcg gctgaaatca
     3061 acctgcgtag cgatattctg aacgcggttg ggatcaccct tgatgaaacc actacgcgcg
     3121 aaaacgtgat gcagcttttc agcgtgctgt tgggcgataa ccacgggctg gacatcgaca
     3181 cactggacaa agacgtggct cacgacagtc gctctattca ggctgcgatg ctgcgcgacg
     3241 acgaaatcct cacccatccg gtgtttaatc gctaccacag cgaaaccgaa atgatgcgct
     3301 atatgcactc gctggagcgt aaagatctgg cgctgaatca ggcgatgatc ccgctgggtt
     3361 cctgcaccat gaaactgaac gccgccgccg agatgatccc gatcacctgg ccggaatttg
     3421 ccgaactgca cccgttctgc ccgccggaac aggctgaagg ttatcagcag atgatcgcgc
     3481 agctggctga gtggctggtg aaactgaccg gttacgacgc cgtttgtatg cagccgaact
     3541 ctggcgcaca gggcgaatac gcgggcctgc tggcgattcg tcattatcat gaaagccgca
     3601 acgaagggca tcgcgatatc tgcctgatcc cggcttctgc gcacggcact aacccggctt
     3661 ccgcgcatat ggcaggaatg caggtggtgg ttgtggcgtg tgataaaaac ggcaacatcg
     3721 atctgactga tctgcgcgcg aaagccgaac aggcgggcga taacctctcc tgtatcatgg
     3781 tgacctatcc ttccacccac ggcgtgtatg aagaaacgat ccgtgaagtg tgtgaagtcg
     3841 tgcatcagtt cggcggtcag gtttaccttg atggcgcgaa catgaacgct caggttggca
     3901 tcacctcgcc gggctttatt ggtgcggacg tttcccacct taacctgcat aaaactttct
     3961 gcattccgca tggcggtggt ggtccgggta tgggaccgat cggtgtgaaa gcgcatctgg
     4021 ctccgtttgt accgggtcat agcgtggtgc aaatcgaagg catgttaatc cgtcagggcg
     4081 cggtttctgc ggcaccgttc ggtagtgcct ctatcctgcc aatcagctgg atgtacatcc
     4141 gcatgatggg cgcagaaggg ctgaaaaaag caagccaggt ggcaattctt aacgccaact
     4201 atattgccag ccgtctgcag gatgccttcc cggtgctgta taccggtcgc gacggtcgcg
     4261 tggcgcacga atgtattctc gatattcgcc cgctgaaaga agaaaccggc atcagcgagc
     4321 tggatattgc caagcgcctg atcgactacg gtttccacgc gccgaccatg tcgttcccgg
     4381 tggcgggtac gctgatggtt gaaccgactg aatctgaaag caaagtggaa ctggatcgct
     4441 ttatcgatgc gatgctggct atccgcgcag aaattgacca ggtgaaagcc ggtgtctggc
     4501 cgctggaaga taacccgctg gtgaacgcgc cgcacattca gagcgaactg gtcgccgagt
     4561 gggcgcatcc gtacagtcgt gaagttgcag tattcccggc aggtgtggca gacaaatact
     4621 ggccgaccgt gaaacgtctg gatgatgttt acggcgaccg taacctgttc tgctcctgcg
     4681 taccgattag cgaataccag taatttactg attcgactat cttctaaagg cgcttcggcg
     4741 cctttttcgc ttgttaataa agtgcgcttt gtgtgtttcg gttgaggcgt aatataaaat
     4801 attgcgagct caatatatta aaagagtgcc aggcctgatg agcggagcac atcaggcaca
     4861 tcggctaatt acaaattctc gccgttgctg gcaatcacct ctttgtacca gttaaagctc
     4921 ttcttacgtg aacgcgacat atcgccagta ccgtcgtcat gtttattcac atagataaag
     4981 ccgtagcgtt tgctgtactg cccggtagtg aacgacacgc agtcgatgca gccccacggt
     5041 gtgtagccca tcagatccac gccatcgtaa gtcaccgctt ttttcatctc ttcaatatgg
     5101 gcgcgcaggt agtcaatgcg gtaatcgtca ttgatgctgc catcatcttc cactttgtcg
     5161 taagcgccaa aaccgttttc gacaataaac agcggcttct ggtaacgctc atacagctcg
     5221 caaagcgcgt aacgcagacc aaccggatca atctgccagc cccagtcaga cgctttaaca
     5281 tacgggttcg gtacgctgcc ttcaaaacca gagatcgcat cgccggtgcc gccttcggcc
     5341 ttcaccgcgt tggtcatgta atagctgaag ccgagataat cgcaggtgcc ttcgcgcagt
     5401 acatccagat cgccgtcttc cattttgatg ttaaatccgc ggcgttccca ctcgttcaac
     5461 acataggacg ggtaatagcc acgcaactgc acatcggtaa agacgtaacg ttcgcgcatc
     5521 gacttctggg caaacatcac atcgtccggg ttacaggagt agggatagag cggcaccatc
     5581 gccagcatac aaccgacttt catctccggg ttgatgcgat gcgcggcttt caccgccagg
     5641 gcgctggcga caaactggtg atgcagcacc tgatacatgg tctcttccgg gttttcatgt
     5701 tcggtataca ccacgccgga gcagcagtaa ccgaacagcg gtgcacgcca gttacgctgg
     5761 ttgttaattt cgttgaaggt catccagtat ttgactttgt gcttatagcg ttcgaatacc
     5821 acttccgcga aacggacaaa gaaatcaacc actttacggt tggtccagct accatattgc
     5881 tgcaccagat gcagcggcat ttcaaagtgg gagagggtga tcaccggttc gatgttgtat
     5941 ttcagcagtt catcgatcat atcatcgtag aacttcagcc cttcttcgtt tggctgagct
     6001 tcatcgcctt ttggaaaaat gcgcgtccag gcgatggaag tacgaaaaca tttgaagccc
     6061 atttcggcaa atagcttgat gtcctccttg tagtgaccat aaaaatcaac ggcttcatgg
     6121 tttggatagt atttgccagg cacaacttct ttggtgattt cgcgcggcac gccgtgtgcg
     6181 ccaccggtca gaacgtcaca aatgctcggc cctttgccgc ctttgttcca gccgccttcg
     6241 acctgatgag cggcaactgc gccgccccat aagaaatctt tcggtaaggt gagttttttc
     6301 actatcatag gctccagatg agtcaattct tacaggaaat attaccatgc agccaaacga
     6361 catcactttt tttcaacgtt tccaggatga cattctggct gggcgtaaaa ccatcaccat
     6421 ccgcgacgag tctgaatcgc acttcaaaac gggtgatgtg cttcgtgtcg gacgttttga
     6481 agatgacggt tatttttgca cgattgaagt caccgcaacc tcaaccgtaa cgctggatac
     6541 gctgacggaa aaacatgcag agcaggaaaa tatgaccctg actgaactga aaaaggtcat
     6601 tgccgacatc tatcccgacc agacacagtt ttatgtgatt gaatttaaat gtctttaaat
     6661 tgaactcacg cctgttagtt gaaatttcgc atttaatttc atgatttttc ggagattaat
     6721 ctcaaatcag aatttatttt agctaacagg tgttcactgg aactattctc agttacgctg
     6781 gagaggtata catgtgcgtg tacgtttttc cggagtaagt tatggttcag aagcccctca
     6841 ttaagcaggg atattcgctg gcagaggaaa ttgccaacag cgtcagtcac ggcattgggc
     6901 tggtgtttgg tatcgttggg ctggtgttgc tactggttca ggcggtggat ctcaatgcca
     6961 gcgccacggc gataaccagt tacagcctct atggcggcag tatgatcctg ctgttcctcg
     7021 cttcgacgct ctatcacgcc attcctcatc aacgggcaaa aatgtggctg aagaaatttg
     7081 accactgcgc tatttatctg ttgattgccg gaacctacac gccgtttttg ctggtggggc
     7141 tggattctcc gttagcgcgc gggttgatga ttgttatctg gagcctggca ttgctgggta
     7201 ttctgtttaa actgaccatc gcgcaccgat tcaaaatttt atctctggtg acctatctgg
     7261 cgatgggctg gctgtcgctg gtggtaattt atgaaatggc agttaagctc gcggcgggca
     7321 gcgttacctt actggcggta ggcggtgtgg tttattcgct cggggtgatt ttctacgtct
     7381 gcaagcgcat tccatacaat catgccatct ggcacggctt tgtgctcggc ggtagtgtgt
     7441 gccactttct ggcgatctat ttgtatattg ggcaggtgta attggcgtgc ctttgtaggc
     7501 ctgaaaagac acaccagtgt cgcatcaggc gataaatgcc tgatgcgata cagagattta
     7561 ctcttccagc gaatatggca gtggctcaat acgcaatgta ttcgcatcgt cacgcacgcg
     7621 gaagatgctg tccggttcca tatcgttatt catgacaacc tgcaccacaa cctgaccatc
     7681 ttccagtttg accgcagcca gcaccgtacc ggtacgacgc cagttctcgc ccattttcag
     7741 ctctaagtct tcaccagctt ccggcagtcg gctggcgcta cccgtcagca accagagcgc
     7801 gcgtttgttg gcaccacgga attttgctcg cgccaccatc tcttgtccgg tatagcagcc
     7861 tttcttaaag ctgatgccgc ccagcgcctg gaggttggtc gcctggggga taaactgccc
     7921 gctgttggcg gcatcaatca ccggaaaacc cgcttcaatg tttaatgcca gccactgttg
     7981 gctattgttc agttccgctt caccgcgcag tttatcggtc agcatattgg cagtagcttc
     8041 atcggttacg atcaggaaac gttctgccgg gtgttcaaac catagcaacg tggtcgcgcc
     8101 ttctttgact acctgttttt ctctcgaagg tagttcacta aagagatttg ccaacgcggt
     8161 gcgcgcctga aaaccggcaa caccaagcag cacacgctcg tcgtctggcg cgatggtcac
     8221 tttagagaac accgcatatt ttttcagttc agtcagctgc ggttcacgca cgctgcgccg
     8281 ttcaatccat gcaaagccat cgccgtcgcg gaacagacgt aaattgctcc acattttacc
     8341 tttggcgtcg caatgggcgg cgagcaggtg ctgatcttca gtcatctggc tgacatctgc
     8401 tgtcacctgg ccttgcatat atttttcgct gtccgcgcca gtaatagtgg caagcgccca
     8461 gtcatcaagc gtcatcagcg tcagtggcaa acgggcagaa gccgtaggct gacggggagg
     8521 aaaaggtgta aaagccataa tagagtcctg agttgcttaa cggcgtatta atcactaatg
     8581 gtaaaagagc tatgaggcaa tgcaagtgct ttcactgcgc gatctgtgcc ttctgcgagg
     8641 cgacttccaa aaaaatgaag cccttgaatt gttgtgaatt aatttcgcga tcgcgattgt
     8701 gtttactgat attcccgtaa aagaagtcga aaaacacgtt acaatagccg ggtactggat
     8761 tttcgtgaga caggaagaag atggacatta acaacaaagc ccgcattcat tgggcatgcc
     8821 gccgtggtat gcgcgaactc gatatttcaa tcatgccgtt tttcgaacat gagtacgaca
     8881 gcttaagcga tgacgaaaaa cgcaccttta ttcgtctgct ggaatgtgac gatccggacc
     8941 tgtttaactg gctgatgaat cacggtaaac cagccgatgc agaactggaa atgatggtcc
     9001 gactcatcca gacacggaac cgggaacgtg gtcctgtggc aatctgattt gcgcgtctcc
     9061 tggcgcgcac agtggctttc cttgctgatt catgggctgg ttgccgctgt tattttactc
     9121 attccctggc cgctcagtta caccccgtta tggatggtgt tactttcgct ggtggtgttt
     9181 gattgcgttc gcagccagcg gcgcattaat gctcgccagg gggaaattcg cttgttgatg
     9241 gacgggcgtt tgcgttggca agggcaggag tggagcatcg tcaaagcgcc gtggatgatt
     9301 aagagcggca tgatgctgcg tttacgttct gacagcggaa aacggcaaca tttatggctg
     9361 gcagctgaca gcatggacga agccgaatgg cgggatttac ggcggatatt gttgcaacaa
     9421 gagacgcaaa gataaagatg acggcaaagc atatgtgcta agccgtcagt gacatcaggc
     9481 gtaatgctct gccatttcgt tgaggatttg ctcgcaccag ctttgaatac gctcgtcgct
     9541 aaggtcatac tggttagttt catccagcgc cagacccacg aacagttgcc cgtcagcaat
     9601 caccggtttc ggactggtaa attcatatcc ttccgttggc cagtagccga cgaacttcac
     9661 gcctttggtc gagagtttgt catgcagcat accgagcgca tcgaggaacc actcgccgta
     9721 tcccagttga tcgccaagcc catacagcgc aacaatttta ccttcgaggt tcaggtcgtc
     9781 gagctgatcc cagacggctt cccagtcttc ctggatttcg ccaaaatccc aggtcgggat
     9841 acccagaatg agcacatcgt actgctccat taatttcggg gagtcgtcct tgaggttatg
     9901 taaggtcacc agttctgggc cgataatatc gcggattttt tctgccgcca tttcggtgta
     9961 acaggtgctg gaaccgtaaa aaagacccat attcataacg taagcttctc gattctgctg
    10021 tggcttttgc ggctagtgta tcagaatcgc tttagtcacg gcacaataag gcataatgga
    10081 tcaaatgagt gaccataagg ggcgcaagtg aaacaggatc tggcacgcat cgagcagttt
    10141 cttgatgctc tgtggctgga aaaaaatctg gctgaaaata cgttgaacgc ttaccgtcgc
    10201 gatctgtcaa tgatggtgga gtggttgcat caccgcgggt tgacgctggc gacggcgcaa
    10261 agtgacgatt tgcaggcatt actggcagaa cggctggagg gcgggtataa agccaccagc
    10321 tcagcgcgtt tgctgagtgc agtgcgccga ttgttccagt atctttatcg cgaaaagttt
    10381 cgtgaagatg atcccagtgc gcatctcgct tcaccgaaat tgccccagcg tttgccaaaa
    10441 gatttaagcg aagcgcaggt cgaacgttta ttacaggcac cattaattga tcagccactg
    10501 gagctacgcg ataaagccat gcttgaagtg ttgtatgcta ccggactgcg tgtctctgaa
    10561 ctggtcgggc tgacaatgag tgatatcagc ctgcgtcagg gcgtggtgcg ggtcattggt
    10621 aaaggcaaca aagagcgcct ggtgccgtta ggtgaagagg cggtttactg gctggaaacc
    10681 tatctggaac atgggcgtcc gtggctgttg aatggtgtgt caattgacgt gttgtttccc
    10741 agccagcgtg cgcagcagat gacgcgacag accttctggc accgcattaa acattatgcc
    10801 gtgctggcgg gtatcgacag cgaaaagctg tcaccgcatg tgttgcgtca cgcttttgcc
    10861 actcacttat taaatcatgg tgcggattta cgcgtggtgc agatgctact gggccacagc
    10921 gatctctcca ccacgcaaat ttatacgcat gtcgctaccg agcgtctgcg acaacttcat
    10981 caacagcatc acccgcgggc gtgatgtctg aaaagaacag gaaaatttat gaagaaaggt
    11041 tttatgttat ttaccttgtt ggcagcgttt tcaggctttg ttcaggctga tgacgcggca
    11101 attcaacaaa cgttagccaa aatgggcatc aaaagcagcg atattcagcc cgcgcccgta
    11161 gctggcatga agacagttct gactaacagc ggtgtgttgt acatcaccga tgatggtaaa
    11221 catatcattc aggggccaat gtatgacgtt agtggcacgg ctccggtcaa tgtcaccaat
    11281 aagatgctgt taaagcagtt gaatgcgctt gaaaaagaga tgatcgttta taaagcgccg
    11341 caggaaaaac acgtcatcac cgtgtttact gatattacct gtggttactg ccacaaactg
    11401 catgagcaaa tggcagacta taacgcgctg gggatcaccg tgcgttatct tgctttcccg
    11461 cgccaggggc tggacagcga tgcagagaaa gaaatgaaag ctatctggtg tgcgaaagat
    11521 aaaaacaaag cgtttgatga tgtgatggca ggtaaaagcg tcgcaccagc cagctgcgac
    11581 gtagatattg ccgaccatta cgcacttggc gtccagcttg gcgttagcgg tactccggca
    11641 gttgtgctga gcaatggcac acttgttccg ggttaccagc cgccgaaaga catgaaagaa
    11701 ttcctcgacg aacaccaaaa aatgaccagc ggtaaataat tcgcgtgaaa caacagatac
    11761 aacttcgtcg ccgtgaagtc gatgaaacgg ccgacttgcc cgctgaattg cctcccttgc
    11821 tgcgccgttt atatgccagc cggggtgtgc gtagcgcgca ggaactggaa cgcagtgtta
    11881 aaggcatgct gccctggcag caactgagcg gcgtcgaaaa ggccgttgag atcctttaca
    11941 acgcttttcg cgaaggaacg cggattattg tggtcggtga tttcgacgcc gacggtgcca
    12001 ccagcacggc tctaagcgtg ctggcgatgc gctcgcttgg ttgcagcaat atcgactacc
    12061 tggttccaaa ccgtttcgaa gacggttacg gcttaagccc ggaagtagtc gatcaggctc
    12121 atgcccgtgg cgcgcagtta attgtcacgg tggataacgg tatttcctcc catgcggggg
    12181 ttgaacacgc tcgttcgttg ggcattccgg ttattgttac cgatcaccat ttgccaggcg
    12241 acacattacc cgcagcggaa gcgatcatta accctaactt gcgcgactgt aatttcccgt
    12301 cgaaatcact ggcgggcgtt ggcgtggcgt tttatctgat gctggcgctg cgcacctttt
    12361 tgcgcgatca gggctggttt gatgagcgcg gcatcgcaat tcctaatctg gcagaactgc
    12421 tggatctggt agcgttgggg acagtggcgg acgtcgtgcc gctggacgct aataatcgca
    12481 ttctgacctg gcaggggatg agtcgcatcc gtgccggaaa gtgccgtccg gggattaaag
    12541 cgttgctgga agtggccaat cgcgatccac aaaaactcgc cgccagcgat ttaggttttg
    12601 cgctggggcc acgtctcaat gctgccgggc gactggacga tatgtccgtt ggcgtggcgc
    12661 tgttgctgtg cgacaacatc ggcgaagcgc gcgtgctggc aaatgaactc gatgcgctaa
    12721 accagacgcg aaaagagatc gaacagggaa tgcaagttga agccctgacc ttgtgcgaga
    12781 aactggagcg cagccgcgac acgctacccg gcgggctggc aatgtatcac cccgaatggc
    12841 atcagggcgt tgtcggtatt ctggcttcgc gcatcaaaga gcgttttcat cgtccggtta
    12901 tcgcctttgc gccagctggt gatggtacgc tgaaaggttc cggtcgctcc attcaggggc
    12961 tgcatatgcg tgatgcgctg gagcggctag acacgcttta ccccggcatg atgctcaagt
    13021 ttggcggtca tgcgatggcg gcgggtttgt cgctggaaga ggagaaattc gaactctttc
    13081 aacaacggtt tggcgaactg gttactgagt ggctggaccc ttcgctattg caaggcgaag
    13141 tggtgtcgga cggtccgtta agtccggccg aaatgaccat ggaagtggcg cagctgttgc
    13201 gcgatgctgg cccgtggggg cagatgttcc cggagccgct gtttgatggt catttccgtc
    13261 tgctgcaaca gcggctggtg ggcgaacgtc atttgaaagt catggtcgaa ccggtcggcg
    13321 gcggtccact gctggacggg attgctttta atgtcgatac cgccctctgg ccggataacg
    13381 gcgtgcgcga agtgcaactg gcttacaagc tcgatatcaa cgagtttcgc ggcaaccgca
    13441 gcctgcaaat tatcatcgac aatatctggc caatttagcg tcatcttctc tataaaaaag
    13501 agcgtggatt gggtacaatc ccgctcttat caccgcattt tgactagctc aataaaagaa
    13561 atcagaccat gtttgaaatt aatccggtaa ataatcgcat tcaggacctc acggaacgct
    13621 ccgacgttct tagggggtat ctttgactac gacgccaaga aagagcgtct ggaagaagta
    13681 aacgccgagc tggaacagcc ggatgtctgg aacgaacccg aacgcgcaca ggcgctgggt
    13741 aaagagcgtt cctccctcga agccgttgtc gacaccctcg accaaatgaa acaggggctg
    13801 gaagatgttt ctggtctgct ggaactggct gtagaagctg acgacgaaga aacctttaac
    13861 gaagccgttg ctgaactcga cgccctggaa gaaaaactgg cgcagcttga gttccgccgt
    13921 atgttctctg gcgaatatga cagcgccgac tgctacctcg atattcaggc ggggtctggc
    13981 ggtacggaag cacaggactg ggcgagcatg cttgagcgta tgtatctgcg ctgggctgaa
    14041 tcgcgtggtt tcaaaactga aatcatcgaa gagtcggaag gtgaagtggc gggtattaaa
    14101 tccgtgacga tcaaaatctc cggcgattac gcttatggct ggctgcgtac agaaaccggc
    14161 gttcaccgcc tggtgcgtaa gagcccgttt gactccggcg gtcgtcgcca cacgtcgttc
    14221 agctccgcgt ttgtttaccc ggaagttgat gatgatatcg atatcgaaat caatccagcg
    14281 gatctgcgca tcgacgttta tcgcgcatcc ggcgcgggcg gtcagcacgt taaccgtacc
    14341 gaatctgcgg tgcgtattac tcacatccca accgggatcg tgacccaatg ccagaacgac
    14401 cgttcccagc acaagaacaa agaccaggcc atgaagcaga tgaaagcgaa gctttatgaa
    14461 ctggagatgc agaagaaaaa tgccgagaaa caggcgatgg aagataacaa atccgacatc
    14521 ggctggggca gccagattcg ttcttatgtc cttgatgact cccgcattaa agatctgcgt
    14581 accggggtag aaactcgcaa cacgcaggcc gtgctggacg gcagcctgga tcaatttatc
    14641 gaagcaagtt tgaaagcagg gttatgagga accaacatgt ctgaacaaca cgcacagggc
    14701 gctgacgcgg tagtcgatct taacaatgaa ctgaaaacgc gtcgtgagaa gctggcgaac
    14761 ctgcgtgagc aggggattgc cttcccgaac gatttccgtc gcgatcatac ctctgaccaa
    14821 ttgcacgcag aattcgacgg taaagagaac gaagaactgg aagcgctgaa catcgaagtc
    14881 gccgttgctg gccgcatgat gacccgtcgt attatgggta aagcgtcttt cgttaccctg
    14941 caggacgttg gcggtcgcat tcagttgtac gttgcccgtg acgatctgcc ggaaggcgtt
    15001 tacaacgagc agttcaaaaa atgggacctc ggcgatatcc tcggcgcgaa aggtaaactg
    15061 ttcaaaacca aaaccggcga actgtctatc cactgtaccg agctgcgtct gctgaccaaa
    15121 gcactgcgtc cgctgccgga taaattccac ggcttgcagg atcaggaagc gcgctatcgt
    15181 cagcgttatt tggatctcat ctctaacgat gaatcccgca acacctttaa agtgcgctcg
    15241 cagatcctct ctggtattcg ccagttcatg gtgaaccgcg gctttatgga agttgaaacg
    15301 ccgatgatgc aggtgatccc tggcggtgcc gctgcgcgtc cgtttatcac tcaccataac
    15361 gcgctggatc tcgacatgta cctgcgtatc gcgccggaac tgtacctcaa gcgtctggtg
    15421 gtcggtggct tcgagcgtgt attcgaaatc aaccgtaact tccgtaacga aggtatttcc
    15481 gtacgtcata acccagagtt caccatgatg gaactctata tggcttacgc agattacaaa
    15541 gatctgatcg agctgaccga atcgctgttc cgtactctgg cacaggatat tctcggtaag
    15601 acggaagtga cctacggcga cgtaacgctg gacttcggta aaccgttcga aaaactgacc
    15661 atgcgtgaag cgatcaagaa atatcgcccg gaaaccgaca tggcggatct ggacaacttc
    15721 gactctgcga aagcgattgc tgaatctatc ggcatccacg ttgaaaagag ctggggtctg
    15781 ggccgtatcg ttaccgagat cttcgaagaa gtggcagaag ctcatctgat ccagccgacc
    15841 ttcattactg aatatccggc agaagtttct cctctggcgc gtcgtaacga cgttaacccg
    15901 gaaatcacag accgctttga gttcttcatt ggtgggcgtg aaatcggtaa cggctttagc
    15961 gaactgaatg acgcggaaga tcaggcgcag cgcttcctgg atcaggttgc cgcgaaagat
    16021 gcaggtgacg acgaagcgat gttctacgac gaagattatg tcaccgcact ggaacatggc
    16081 ttaccgccga cagccggtct gggaattggt atcgaccgta tggtgatgct gttcaccaac
    16141 agccatacca tccgcgacgt tattctgttc ccggcgatgc gtccggtaaa ataagcatta
    16201 cgttatgctc acaaccccgg caaccgtcgg ggttttttat ttaagttggg taaatgcaga
    16261 taatcgtttt ctggcttcgc gatttgtcgc ctgcatcacc atccacggac tgaacgccca
    16321 cggcgtagca tcaataccgc gtaaaaccgc tgctaaatca caccattgat aatccatcac
    16381 ttcatcatcg ttgatctgta acgcactggt cgtgcgtgcg gcaaataccg gacacacttc
    16441 attttccaca atgccattcg gatcggtggc gcggtagcga aagtcaggat agatagattc
    16501 aggaggcgta atttctacgc caagctcata acggcaacgg cggatcaccg cctcttcgtt
    16561 gctttctccc agttgtgggt gcccacaaac cgagttagtc cacacgccag gccatgcttt
    16621 tttgctaagg gcgcggcggg taactaataa ttgccctttg gcattaaaca gccaactgga
    16681 aaacgcgaga tgtaagaggg tgtctgccgt gtgtgcggca tacttttcca gcgtacccgt
    16741 gggaactccc tgtgcattca ataaaatgac gtgttccgtt tgcataattt ctcacatgta
    16801 attctgatca ctcatcatta tagataatag aagagtgaaa tgattaacca agtgagcgtt
    16861 tatcgacaac cgcccgtttt gagcggatgt cgacaggtaa aaaccattta aaaaagcagc
    16921 atcatatatt tgaagcgact ttcaatggcc attgcaacac aatcagaaat gacaggaata
    16981 gagagagtaa aaagcaacag cagactgaat atagcgcgca ttaaaagatt catattattc
    17041 tccatgctca tttttcttca gcttgcgcat cagaagccac atacgtactg tcctgacttt
    17101 taaatcttta tcagagacgg attccttcac caggtcagca tgatgctggt agccattaaa
    17161 gaagacgttc agcacaacgg cgctaagggt tgcgagcata atgccgctat gcagcaacgg
    17221 ttgcagtacg gccggtaact tagaaaagaa atcgtgagag agcgttggcg tcatgccaac
    17281 accgagactg atcgccacaa tatagaggtt gtaacggtta gtggtgtagt tacagcgcga
    17341 cagaattcga atccctgtcg ccagtaccat gccgaacatc accagccccg cgccgcccag
    17401 cacaaattgc ggaatggagg cgaccagcac cgccattttg ggcaccatgc caaacaggat
    17461 taatataatt cccgaagaaa tacacaccca gcggctgtgg acgcgcgtca cgctgaccag
    17521 cccgacgttc tgcgaaaatg acgtatgtgg gaagctgtta aacgtgcctc caatcattgt
    17581 cccgacccca tctacgcgta acccacgaat aatgtcctgg gaagagagtt tgcgaccgac
    17641 tatttcaccc agtgccagga acatccccat tgactcaata aatacgatga tgagtactgc
    17701 ggtcatggtc aaaatggaaa cgggatcgaa aatcggcata ccaaatgaca tcggcgtgac
    17761 aatcgcaaac catgaagcat catgtagccc ggataaattg acttcattca tcatccacga
    17821 aagtaaaaag ccaaatacaa tccccagtaa tacggcgacg ttggacataa atcctttcgc
    17881 atagcgagta atgagcaaga taaaaattaa gacggcaaag gagataccta aataaacggg
    17941 attaccatat tgcggattcc ctttacctcc ggcggcccag tcaataccca cctgaatgat
    18001 gctaagcccg atagaagtaa tcaccacacc ggtaaccagt ggcgggaata aaggcatcaa
    18061 gcgaccgata agtggcgcta ataatgtggt gataaaaccc gcggcgatag tggcaccgaa
    18121 tatccccagc aggccgatat ccgggttcat accaatggct atcattggtg ttacagcggc
    18181 gaaggtcacc gacataatca ccggcaggcg gatccccata aagcggccga taccgataca
    18241 ttgcaataat gtgacgatcc cgcagcaaaa gagatccgag ctaatgagca tcgcaatagc
    18301 ttcttttgat aggccgagtc ggtcaccaat cataagagga acagcgactg cacctgcgta
    18361 catgacaagg acgtgttgca gaccgagtat gatcagcttt cctggtgata atatgcggtc
    18421 aacctcatca gttgggcggt cttgcccaga agatgaggga agttgggaat ctatggcgct
    18481 catcagtgtt tctcctttcg cccgccagag taaaaagcaa ccgtttatgt tcatgaaact
    18541 gtctaaacac atgctcgtgg agtgccagtt tttgcgagga aggggagtta gagacaggaa
    18601 ttgcggaggc aatcacataa tcgtggttaa accacgaaaa tgagaagaga acctgacaat
    18661 ggaattatct gtaattttct catattgata tttcattaat cattctggca tgtcgcactc
    18721 tcgcatttaa tcgtttttat ctggatagca ttcttttgat cggcaatgtt gaaataatga
    18781 aagcggcata gattgagggg caataatttg aactcagttg caaaattata attaaaacgg
    18841 tcatgctacc taatatatgc ccgtttattg catcgcgaaa tttgagaaga cataactcat
    18901 taaatgaaag ggatgcaaat gaataagttt atcgctgctg aagctgcgga atgtataggc
    18961 tgccatgctt gtgaaattgc ctgtgcggtg gcacataatc aagaaaactg gccgctgagt
    19021 cacagtgact ttcgaccgcg tatccacgtt gtagggaaag gccaggctgc gaatccggtg
    19081 gcctgccatc actgcaacaa tgccccttgc gttacggctt gtccggttaa tgctctgact
    19141 ttccagtccg atagcgtaca actggacgag caaaaatgta ttggttgtaa aagatgcgca
    19201 atcgcttgcc cctttggcgt cgttgagatg gtcgatacga ttgcacagaa atgcgacctt
    19261 tgtaaccagc gcagttccgg cacgcaagcc tgtattgacg tctgcccaac acaggcgtta
    19321 cggctgatgg acgataaagg gttgcagcag ataaaggtgg cccgccagcg caaaacggca
    19381 gcaggaaaag cgtcatcaga cgctcagcca tctcgcagtg cagcgttgct ccccgttaac
    19441 tcgcgtaaag gcgcagataa aatttcagcg agtgaacgga aaacccactt tggcgaaatc
    19501 tattgcgggc tggatccaca acaagcgact tatgagagtg accgctgtgt ttattgtgcc
    19561 gaaaaagcta actgcaactg gcattgtccg ctgcataacg ctattccgga ttacatccgt
    19621 ctggtacagg aaggaaagat tattgaagcg gcagaacttt gccaccagac cagttcctta
    19681 cccgaaatct gcggcagggt atgtccacag gaccgtcttt gtgaaggtgc atgtactttg
    19741 aaagatcact ctggcgcagt ctctatcggt aatctggaac gctacatcac cgatactgcg
    19801 ctggcgatgg gctggcgtcc cgatgtcagc aaagttgttc cccgtagcga aaaagtggcg
    19861 gtgattggcg ctggacctgc aggattaggg tgtgctgata ttctggcgcg cgcgggagtt
    19921 caggttgatg tctttgatcg ccatccagaa attggcggta tgctgacttt tggcattcct
    19981 cctttcaaac tcgataaaac ggtattaagc cagcggcgag agatattcac cgcaatggga
    20041 atcgatttcc atctcaactg tgaaattggc cgcgatatct ctttcaacga attaacggcg
    20101 gaatatgatg ctgttttcct cggcgtgggg acttacggga tgatgcgagc agatctgccg
    20161 catgaagatg cgcctggtgt cattcaggcg ttaccgttcc tgaccgccca tacccgccag
    20221 ctcatgggat tgccggagtc tgcagagtat ccgctgacgg atgtggaagg taagcgagtt
    20281 gtggtattgg gcggtggcga tacgacaatg gattgtttgc ggacttccat ccgccttaat
    20341 gctgccagcg tgacctgtgc gtatcgtcgt gatgaagtca gtatgccggg ttcgcgcaaa
    20401 gaggtggtca atgcccgcga ggaaggtgtt gagtttcagt tcaatgttca gccgcaatat
    20461 atcgcttgtg atgaagatgg acgcttaacg gcggtgggcc tgattcgtac cgctatgggt
    20521 gagccggggc cggatggccg ccgtcgtcct cgtccggttg cgggttcaga gtttgaattg
    20581 cccgccgatg ttctcattat ggcctttggt ttccaggcac ataccatgcc gtggttgcag
    20641 ggcagcggaa ttaaactcga taaatggggc ctgattcaaa ccggcgacgt cgggtattta
    20701 cccacccaga cgcatctgaa aaaagtcttt gctggtggtg atgcagttca tggcgcagat
    20761 ctggttgtca ctgcaatggc cgcaggaagg caggcggcgc gcgatatgtt aactctgttt
    20821 gatacgaagg catcgtgatg aaatcgttaa ttatcgttaa tccggctgac tgtattggct
    20881 gccgtacttg tgaagtggcc tgtgtggtcg ctcacccttc agaacaggag ttgaatgccg
    20941 atatcttttt accccggctg aaggtacaac ggctggatag catcagtgcg ccggtgatgt
    21001 gccatcagtg tgaaaacgcc ccttgtgttg gcgcttgtcc cgtgggggcg ctgacgatgg
    21061 gcgagcaggt ggtgcaggca aattctgccc gttgtattgg ctgtcagagt tgcgtcagtg
    21121 cgtgcccgtt tggaatgatc accattcagt cattgccggg ggatacccgg caacaaatcg
    21181 tgaaatgcga tctctgtgaa cagcgggaag aggggccagc ctgcgttgaa tcttgcccga
    21241 cacaggcgct gcagttgctg actgaaagag aactcaggcg agtccgccag cagcgtattg
    21301 ttgccagcag tgagaatcct ctgtgaaaga aatctccgcg ctgaaaggcg cggagaatct
    21361 attagattgc ccaaccaccc gcgtaaaacg cgaccagtgc ggcagtaatg atcaccgtac
    21421 caatattcag cttttgccat tcgcgcgcaa agacgcgacc caccaccagt gttacaaagc
    21481 ccagcataat accggtaacg atattacaag tcagaacgat gaacacggca cacaccaggc
    21541 cagccattgc gtcaataaaa tcgttgaaat ccagcttcga gacgttgctt aacatcagca
    21601 aacccacgta catcagtgcg ggagctgtgg cataaccggg gatcagaaac gataacggtg
    21661 ataaaaacag aatcatcagg aacaacacac caaccacggt tgcggttaaa ccggttttcc
    21721 ccccggcggc ggttcctgcc gctgattcga tataaaccgc cgcgggcgct gcgcccacca
    21781 ggccggagaa tattgaactt actgagtcac tggtcagggc tttgccgccg ttgatgatct
    21841 ggttgtcttt atccaacaaa ttcgcctgac cggcgacggc acggatggtg ccagtggcgt
    21901 cgaacactgc ggtcatcacc aatgccagta cactcggaag tacagttggc tggagtgcgc
    21961 ccataatatc gaggctgaaa atcagagact taccatcttc gccagtcagg cttggcatcg
    22021 ccaccagacc gtggtacttc accgctgggt caaagattaa gccgatgatc gaaattgcaa
    22081 taatcaccaa caagatcccg ccgggtacgc ggcacttttc cagaccgaag atgaccgcca
    22141 gccccagtaa gctcatcatc accgggaagg aggtaaaggc accgagcgcc actggcaagc
    22201 cttcaatcgg gttttttata accattccca caccgttagc agcaatcagc agcagaaaca
    22261 ggccgatacc gatacctgta ccgtgagcga tccccatcgg caaattacgt aagatccagg
    22321 tacgcacacc ggttacggaa atagcggtga agatgacgcc catcagaaag actgcgccca
    22381 gtgcgacagg aacgctaatt tgttgcccga gtaccagact gaatgcggta aacgccgtca
    22441 aggaaatagc gcaaccaatc gccattggca aattggccca taaccccatc agcaacgagc
    22501 cgaagcccgc gaccagacag gtggcaacaa acacagctgc gggaggaaaa cctgctttgc
    22561 ccagcattcc cggaacgacg ataacggaat aaaccatggc cagaaaggtc gttaagccag
    22621 ccagtacttc ctgacgaacg gtactgccac gagcggtaat tttaaaataa ttatccagcg
    22681 cgccctgtgg ctttggtgcg tccggtgttt gtaggatgtc tccagacata atgatgtcct
    22741 cacgaggatg ttaaagtttt attattagtt gcgttcgtac accagacgac catcaacgta
    22801 ggtgcggtag atcgaacggt catcgcccaa cgtcatcatc acgaacaatt tgtcgactaa
    22861 agaaacagag ttgtcatagc gcagctgctg tagcggagtg gcagtgggtt ccatcaccac
    22921 gaaatcagcc tctttgccag gtaaaaagtt gccaatcaaa tcgtcaaggc ccagagattt
    22981 cgctccgccg agcgtggcca ggtaaaacgc ttcatatgcc gagaggcgat agccttgtaa
    23041 ttgcaatacc ttgtaggctt cgttcagcgt ttgcagcatg ttaaaagttg ttccggcacc
    23101 gatatccgtt cccatgccca ctttgacttt cttctgccat gcttttttca agttgaataa
    23161 gccgctgccg aggtaaaggt tggaggtcgg acagaaagca atgctggatt tggtttcgct
    23221 gagacgatcc cactcttttt cttcgagatg gacgcagtga gcaaagacgc agtttttacc
    23281 ggtcaggccg tactgatggt aaacatcaag ataaccatca tggtcaggat aaagcgattt
    23341 cacccaggca atttcatctt tgttttcaca gagatgggta tgtacccatg tatccggata
    23401 ttcttctttc aggcgttgcg ccatcgccat ctgttcagga gatgaggtag gggcgaagcg
    23461 tggcgtaatc gcatacaaca gacgaccatt tttgtgccag cgttcaatca gttctttgct
    23521 ttggtgatag ctgctttcgg cagtgtcgag cagataatcc ggtgcgtttc ggtccatcat
    23581 cactttaccg gcaatcatac gcatattgat atgactggcg gcttcaaaca gcgcatcaac
    23641 ggattgtgga tgaacagtgc caaacaccag cgccgtggtg gttccgttac gtaaaagctg
    23701 cttgatgaag aacgccgaca tctcgcgggc gtactctaaa tcctcataac gacgttcagt
    23761 agggaaggtg tgtttattca accactccag caattgttca ccataggccc ccaccatttc
    23821 actttgcgga taatggatat gtgtatcgat aaagcccggt actatcagtt tgccgcgata
    23881 gtcgcgcacg cgaatggtgt caggaatttg atgctttccg tcttcccatt cgccaaacca
    23941 ttccactttt ccctgtttaa tgagtaataa accatcctca ataaaccgca gcgcagaggc
    24001 aatttcttcc gggttatcga ccgtacgggt gacatcaata aaactgcctc gtaccgcttt
    24061 taacgtgtgt tctcctgaca tcattgactc ctttactgtt aatccatctc ttccgctgag
    24121 gtaataccag gcagaacgtt ttcctgtcgg tagccaccag ggaggataat atttaataaa
    24181 atcgcagtta acccgccagc acaaataggg ttttcaacta atacataaat agaggctggc
    24241 aatattttaa aaatttcggg atcgtaggag ataccaagcc ctaaacctaa agaagtggca
    24301 acaataagcg tttcacggcg ctttaaaccg ttggtgatga tgatgcgaat ccctgcgatg
    24361 gcaatcatgg aaaacatcaa cgtcattgcg cctcccagaa ctgctgaggg aatggtcgtg
    24421 aagaagccgc caatcatcgg aaataagccg aggataacca gcattaccgc gatggttcgc
    24481 ccgacataac gtgaagcgac gccagtcatc tgaataaccc cattattttg cgcaaacgtg
    24541 gttaatggta atgaaccgac agcggaggcg ataacagaaa ccagaccatc tgccagcacg
    24601 ccgcctttca gtcgggactg atactcttcc ccctgaatgg gacggcggga aaccattgcc
    24661 gtggcggtga tatcgccgac agcttccagc acgctaagca gataaatcgt gccaaccacc
    24721 aggaactgat ggaagctaaa actaaagccg tatttgaacg gatgcgggat ggtgattaac
    24781 ggcaaattgc gcatactgct gaaatccacc atgcccaggc ataacgatgc gatatagccg
    24841 acacatagcc caatggcgat ccctcccatg cgtagcaacg gactgcgaca gcagttaaag
    24901 ccgatcacca caatcaaaac caataaacca acgccgagat gttcgtaatt gccgaacgta
    24961 ccgctgcttt tggctgcaaa tcctccacca aaatcaataa tgccgacttt aatcaggctt
    25021 aagccgatca tcagcaccac aatgccgctg acggtaggcg taataacccg acgtaaatag
    25081 ggcaggataa aagaagagcc gacaaccaga aatgcgccaa cgaaggagac gccgaggagc
    25141 gacgacatga ttaactcttc gtgaaaaccg tcgcttttca tgctgctgcc cagcgcaatc
    25201 atgaccgtaa caaatgaaaa attgactgac tgaattgaaa gtaaaccaga accgacgatg
    25261 ccgtagcggt ttacttgtaa ccaggtacca ataccagagg cgatcatcgc catagaaaca
    25321 agataggcag ttgtttcagc ggaaagctgt aaggccgcac ccacgattaa cgcgggggtt
    25381 accatcggaa cgaaaattgc caacagatgg gtaatggcac ctacgagagc ctgatgaaag
    25441 ggagggcgat cctccagttc aaatataagg tcagaacctg catggtttat atcagacatc
    25501 ccttcactcc ttttaaagta aatttgatca tctgaattag taaaataaat tcccacgaat
    25561 ttggggcgca ggacgccttg agaacggctt atctcatttt cgcagtcact atttagtcat
    25621 cgttcaaaca gagaaacagg ccgccgaagc ggccatttat tacttatatt ttttccagcg
    25681 cggtgagtat tttctccggt gtgaaatgcc attcgcgtaa ccagatgccg caggcatcgt
    25741 gaattgcggt agcaatcgcc ggagctgcgc catttacacc gatttccgag atcgatttcg
    25801 ccccgaatgg gccgacttta tcgtcgctcg gcaccagcac agcgcggaaa tcacgcggaa
    25861 tgtcaccaat tttcggtgcg ccgtaactgc gtaaatcacg cgttaacggg tgaccttcgg
    25921 cgtcataaat gatctcttcg ctcatactgt ggccgatagc acgcagggtg gcaccgtaga
    25981 tttgtcccaa cgctaactct ggattgaccg gtgtaccgca gtccagcaag gcgtagaatt
    26041 tatccaggcg gatttcaccc gtacgcgtgt tgacggcaac ttcagcgaag tttgcgccat
    26101 acgggaaggc gaaatcaggt gtgatataac tgccagttcc caccagtgaa ccaaagccgg
    26161 tgccggtttc gcctttatgg gctatctctc cgaaactaac ttcgcctttc ttgccgcgaa
    26221 cgacgcccgg cgttgctagt tgaacatctg ccactggctc acccaatatt tgcgcgccgt
    26281 ggaacaggat tttctcccgc agattttccg ctgccaaacg cgcagcgtta cccgagaagc
    26341 aagtaccgga cgaggcatat gcgcctttat caaacagtgc atgatcggta tcaccagaga
    26401 taacatgcac gtcctgcggt gggcagtgca gcacttctgc tgccagtttg gtcaccacag
    26461 tatccagacc agtaccaata tccgcaccac cggaatgaac gataaaggtg ccatccgatt
    26521 ccagtttgat catgcagtta gcctgatcga tatccgggat acccgatttc tgcatgataa
    26581 tggcgacgcc gcgaccgatg tgccagtcac cattttgcgg ttttggtgaa gaccattgga
    26641 tcatctcgcg accctgacgc aggatctctt ccagtgcgca gctggcggcg gaaggaacgg
    26701 aggtcggcgc tttaccttca ccgattgcac cgagaatttt cagctcttgc ccttcgtgta
    26761 cccggttacg ttcgataatt tccagttggt cgatctgtaa ctgttcagcc agttccgcta
    26821 atgccatggt gatagcgaag ttacctttcg gtgcgccata accctgataa gcaccatttg
    26881 ggcaaatgtt gctgtagtag gtggtgacct gaaaatcgac gttatcgcat ggatataacg
    26941 gcagcgacag cgccggtccg ttacacggta cggtgagtga gtggttaccg taaggacccg
    27001 tgttggcgcg gaaatccatc ttcactgccg tcaggcgacc atcttttttc gcgcccagtt
    27061 tgacggtgac tttcgcgacg tgacgagagg tgttagcaat aaactcttct tcacgggtgt
    27121 agcggaaaag taccggacgc ccggtcacgc aggttgccca ggcgcacact tcttccagca
    27181 ggatgtcctg tttggaacca aaaccgccgc caactcgctc tttaatgaca tgaactttat
    27241 gctgtttcat gcccacgagg cgcgcgacct ggcggcgtaa gtgccatggt acctgggtgg
    27301 aggcgtggat caccagacga tcgccgtcca tacgggtaaa gcagatatgt gtttcagtcg
    27361 ggcactgctg tgcctgcgtt gaattatagg ttcgctcaat gatcacatcg gcatcggcaa
    27421 agcctttgtc catatcgcca atatgaccat gaatactggc ggcgatattt ttgcgtgggc
    27481 gagaaccgat cgggaagttg atgatcatat gctcgccgcg ctgggctgca tggctgttat
    27541 catcttccag agtatctggc gcaccagcaa catacaccac cggttcatcg tgcacgacag
    27601 gcgcatcttc cgccattgcc tcgtcaatcg acattaccgg cttaagcact tcatattcaa
    27661 cgtcgatgag cttcagtgct tcgagcgcaa tgtcttcact ttcggctact accgcagcaa
    27721 cgcgatcgcc gacgtgacgc attttcttgc cgaacattcg gcggtcaagc ggtgacggtt
    27781 ccggtgcgct ctgaccaccc ggggtatagt aaatatccgg gcaattcagg tgagtaataa
    27841 cgtgaacgac gcccggtaag gcttcagctt tgctgacatc cagatgagta atcagtgcgt
    27901 gagcgtgtgg gctacgtaac attttaatga cgcaggcgtc agccgttacg cgatcttcaa
    27961 catagcaggg tttcgcctgc accattttcg cggcatcagt tttaggataa tgcttgccaa
    28021 tgacttcaag gtcgtcacgg aaagtcggag cgatatcaat ggtggcctgc ggattatttt
    28081 tacgtgcaac cgccagttca atgacctgat agtattgctg ccagccagca tcgcggctaa
    28141 acagacctga gagtgcgtcg tcgatctctt cgcgggtagg tgcgtcgatg cgatcgagaa
    28201 gatcggtgat aatcagagcc gcagctggat cgttataacc agactgcacc acgccaacat
    28261 caaccatggc ctgttgaacc agacttaact cattccattt gcccagagat tctgcggtac
    28321 gaatatctgc cttctctaac tgtgcggcga taagcaagga cgcgttaacg atattaccgt
    28381 taaagataat tgcgtcagaa ccggcaaacc caaagccatc atcactgttg cgtacagagt
    28441 gcattcccat gttaaacaac agcttttgca cgttttcgcc tggattaacg gttagctcct
    28501 gaggcgcgcc atttaaagta aagtggatga tcatacggct tcctccccag cctgttggca
    28561 gtcggcatac aggtcggcta ccagaactcc cgtgatatag cgtttatagg ccacgctgcc
    28621 ccgcaaatct tcctgcggga aaatggcgtt ggcgacagcc tgttccagtg cattgccttc
    28681 cagattttga gtttcaacat catgcagacg cagtgcttta ctggcgacgc catccagcgc
    28741 aattcgcata ccgtcgtggt ctgttattgc aacggctgcc gtcacgacgg ttaaacctgc
    28801 ctgagaacgg ctaattttgc gagtcgcaca ggtgcgatac ggatctttaa tgataatttc
    28861 ggttaacagg cgatcgcatg ggcaggccag gtagtcctcg attgacagcg tttcgccgtt
    28921 gccaaaaacc agttcagcat ccagtgccag caggacggga agcagcactg actcttcttg
    28981 ccgggcggca atttcaccac caatggtcga ctgattacga acatggcgag agtagacaaa
    29041 accgagagct tcccgcagtg ctgcaggaat aaatcgcgca tcacgcagtg gctgcaagcg
    29101 agacattgcg ccaattcgta gtgcaccgtt atcccagtca atccagtcca gctccagatc
    29161 ctgcaaggaa atggcaatct ttttatcggt acgggttggt gtagcgttga gtttgctgcc
    29221 cccggcgaac cagacggctt catcctggta gcggcgcttc agttccagcg cctgttcgac
    29281 agagtcgggt ctgaaaaatt gttcaatcat cgtctttcct taatttcccc tcttctctga
    29341 aagaagaggg gagctgttat ttatgccagc gcatccatcc gacgccacat actggcagcg
    29401 gcttttctgg cttgcgcata aatggaatcg caatcgaagt taaactgacg gtcttcatag
    29461 accatcacac cattgaccat cacgctgtga acgctacctg agcccatacc gaaagcgata
    29521 tgcccggcga tattgtctgc cagcagcggc gtcggcgagt tgtaatcgca aatggttaaa
    29581 tcggctttgt aaccggcttc cagaagccca aatttcgcgc caaagttgcg gctcatcagt
    29641 tcgttgccgt tagccagtgc tttggcaaaa ctgtcaggcc acagcgggcc acccgcatcg
    29701 cgatgtttaa agaaggcaaa tttcatctct tcaaacatgt ccgaaccaat gccgtccgtt
    29761 cccaacgcca gattgcggat gtcgctaagg tgatggttgt agccgacatg gttgttcatg
    29821 tttgaacggg cgttatgcac caggaacgca tcgcgctgat tgagtaggac gatgtcatct
    29881 ttcgacaggt acagcccatg agcgaccagc gttttgctgt cgatgagatc gaattgcgca
    29941 agtcgtgcca gcaggtcttt gccgtaccaa tggtgactgt aggaaacgtc gtaaaggtct
    30001 tccgcaacgt gaatatgcaa accacgacct gtgcttttca cggcttcacg cagcatctcc
    30061 agaccggcat ccggcacggt aaacggcgcg tgagcgccga tatgtgcttc caccagatac
    30121 ggctcggttg ctgctttctt cgcctcatca atctgacggg tgaagcggat gttttcttct
    30181 acaccttcct gcaactcttt gatgccgttg ttacggtcag tagtttcaaa acaggtcatc
    30241 gcgcgcaggc caacttttaa aaatgcgttg cgcaatgtgg agagcgaccc gtcgatatac
    30301 gccggagagg catggtgatc gataaccgat gtacatccgc tcttaatcgc ttccagggaa
    30361 caaatcagtc cgctgtaata gagcgactct tcatcaagag cgcgatcgag ccgccaccag
    30421 agatttttca gcgttgagat gaaatccggg cagggggcga tgtttgccat aattccgcgg
    30481 gaaagccccg agtaaaaatg gttgtgcgag cagacaattc ccggcatcac aatccgacca
    30541 tgcatctctt tgtagctggc atcggggtag cgttgcgtca gggcatcgcc gatagcgaca
    30601 atcacatcat tttcgatggc gatatcaacg ccttcctgca ctttcgccgg gtgtaactgc
    30661 actgcggtga cattcttcag aatcaacatg attacacctc cacgcggccc agcagataat
    30721 gatgatgctg gtggacatgg ctaatgatgc ggcacatatc gttcagctcc ggtggtacgt
    30781 tgttgaactg accttcgctg tcgatgttta acacccagct ttggttattc agacgtacgc
    30841 gtacccggca atcttccaca aggaagcctg ggttactgct gttatcaaag tcttgcgaca
    30901 ggctgaagac ggtgattttg tctttgtacg gtttaccgtt ccacgggcag aattgggcgc
    30961 agttgccgca ttcgttgcag taggcatcga gatgcagcgt ctggaaacgg ttctggaagc
    31021 caggaaccgc aatggatacg ttggcgcggt tcgggcagac atccacacac ttgctgcaaa
    31081 cgtagttaca ttcaaggcag cgtgcggctt cctgtgcgac aaacgcgtca cgatcgtcac
    31141 tgttcaccag agtgacagag atatcgcctt tacgttgata gatttccgcc ggatttacgt
    31201 tgttccagta tttatcgttc tggtgggaac ggatattttc ccgactaagg atggtatcgg
    31261 tcgcccgacg cgcggttccg acagcagcga caatggagga tggtccgcgc tgtacgtcgc
    31321 cgatcataaa gacgtcagtc agacgcgttt cgccattatg gtcgacgtct ggccagccgt
    31381 ttttgtccag cggcacgccc atcgcattca gggcttcagt atcctgctgt tcaccaatgg
    31441 cggtgatcag actgtccaca tgcagtgtta ctgtttcgtt ggtttcaacc ggacggcgac
    31501 gacctttctc atcaggttcg ccaagcgaca taacgcgcaa ggttaaggtg ccatcagcat
    31561 cgaaacgttc cggattattc agaaaacgga actccacgcc gtcgtgcaac gcttcttcat
    31621 actcttcgcg ccatgcgggc atttcttgca gtgaacgacg gtaaacgacc gttgcttttt
    31681 ctacgcctgg aacgcgtaac gccgcacgag cgcagtccat tgcggtgtta cccgccccga
    31741 caacgaccac atgtttgccc agcttgagcg cggtgccctt gttgtattca cgcaggaaag
    31801 ggagtgattt ccagacattt tggttgtcgc ccgccagttt cacaccgcta tttttatcag
    31861 tgccggtggc aatcagaaca tagtggaagc cctgattttt taactgttca acggtcaaat
    31921 cgggtgagca accgtactca aatttcacgc cgtgagcggc aacaaaatcg atatcgtgct
    31981 gaattaactc tgcaggaata cggaactgag gaatgatatt tttcaccacg ccgcccgcat
    32041 tggcttcgcg ttcaaacagc gtgaccggat ggcccgctct ggcaaggaag taacctgcgg
    32101 ccagacccgc cggacctgca ccaatcacgg ctaccggatg gcgtgaaccg gaaccggctg
    32161 gtttgtgcca gcgttgctta tattcatccc aacctttttc cagggcgact tttttcagtt
    32221 cgcggatatt cagcgcactg tcgtaatcca ggcgggtaca gttgtattgg cactggtgat
    32281 cgcaaatatg accggtaatg gcgggcaggg cgttgcgttg atagatgagt tccagcgcgt
    32341 cggcatagcg gtgttcgcca agcagacgga tgtattccgg aatatcttgc ttgatagcgc
    32401 aggcagtaac acagggggca acgtagcaat cggtcagcgg caggtcttca gccacttcaa
    32461 tacgctcttc tggcttccag tgtttctggg tgtattccat ggttaacgcg tctgctgcca
    32521 gtctgttcag tcgttcgacg tcaacgtggt taagctccca ggcgtcggag ccttccagct
    32581 cgcgcatgca ggcacttaag cgcagatagc cgccaggttt cagcaggtcg gttgccatag
    32641 taatagggcg aataccagta tcaaaaatat cgcggatagt cagctgactg gcaccaccgg
    32701 aataagaaat gggcagtttg ccgtcaaagg cgcgagagag aactgctgca acgttgatgg
    32761 agagcgggaa cagcgcacgg cctgacatat acatctcttc accgggcagt gcgcctttat
    32821 tgttgatggt gccgagagtg ttagtcagtt ttacgccaaa gccgagtgat ttttcttttg
    32881 ccagtgccat caggcgttcc agcatttcca gtgcttgcgt cagcttgagg tcgtgatcaa
    32941 atgactcttc ttttaagccg atgtaaccga aaccgcagac atcgaggatc tcacgaacac
    33001 gcgcgtaccc cagtaaggtc gggttgagtt tcacaaaggt gttgagtcct ttttcttcca
    33061 gcatgtagcg gcaaatggct tcgatttcat gcggaggaca gccgtgcatg gtggagaggg
    33121 taacaccttg taccatgctg gtggggatgc gagcgggtaa ggcttgcaag ctttcgcgtt
    33181 tttcctgcaa tccgtggcgg gctaaaaatg cctcatcctg gagtagctta ttaagtgcat
    33241 cgcggtattg agcgaatttc ggatggtcag atgcgtccat catattgtcg atgaactgct
    33301 gcatcggcgg ttgcttaata ccttcgaggt tgtaaccgac gctcatatta aagatgaacg
    33361 atttaccgga atcagaaggc tggaacatcg cttcgagaag gtgcagggca aaccaggctt
    33421 tgaggtattc atcccaggct ttaagcaggg taaattcggt agaccattcg gtgttaaagc
    33481 actcgtcttc ggcatcgata cagggctttt ccagctccag acggtcaaga atttggacgg
    33541 tttttagttc gatgaagcgt ccgccagtca gccaggaagt gacaatattt tgcgcgagtt
    33601 gcgtgtgcgg gccagcggca gggccgacgg gagtggcaca ggtttcaccg aagacgctaa
    33661 cagttttact ttttacgggt gagtaaaact gttgctcggg aataccaaag attgagcgtt
    33721 gttgttggta ttcatcaaat atgcgcgtca aaagttcctc aaacggaatg ggacgcataa
    33781 tatcccccat aaccctctcc ttagcttata cagatatcat tgagtgaccg gtgttgagaa
    33841 atccggtggt ttagggggat agagcaacaa tcgcaccact cttgccaggt ttgacgggaa
    33901 atataaaaaa atgtgaggcg gctcgaaact taatggcttt ttgtgaatga actcacaggt
    33961 gccaaatgag aattaaaatc actcctgaaa attattattg ccagcccatt tatcactttt
    34021 tctcttttct caaaatcagt gattccttat cattatgata aagagtgctg gagggattaa
    34081 atatttgaac gcaatcgttt aactacgttt tctaaatttc agtatacctt ttcttcgctg
    34141 taataaagtc gtccggagta tcaatatcta aaattatttc tgcattttct atttcgacgg
    34201 aataatgttc tcccataagg agtgcctgac gcatattggt gacattgggg cgctggattg
    34261 cctgcatcag gcatggtttt gacactaaaa tcggatggcc ggggatgcca ttatggagtg
    34321 gcagtattgc gccatcattt cgtaacgacc agatttttct aaaaatatcg atggtgaggg
    34381 ttggcatatc tccgtgggtg agaaaacaat gttctgtttg caccgcgggt actgcggcct
    34441 ttactgatgt cagtaaaccc tgcgcataat ctgggttgtg gataatagtg atattgctct
    34501 ggttcgcata acgttcgtgc agttcattac cacgatagcc ggtgactaaa ataattcggc
    34561 tacaaaactg caacgcattt ttgatacttg tatcaagaat tgttccctgt tgccagggta
    34621 acatcatttt ccattgcccc attcttgatg ataatcctgc ggcggtaatt atacagtcga
    34681 tggctgacat agtgattcca ttatttacag gagctaataa cgcctgataa ttgcgttact
    34741 tcctgatgga ttacgttgag ggtaatttat cccagagagg tccataaaga cgcgtgaaaa
    34801 gtataattga cccatcggca ttattcattg atttaggcgc gcagaaacgt cccactgtga
    34861 tttctattgt tggtgctggg gggaaaacca gcttgctttt ttggctggca gaactgttcc
    34921 aggcgagtgg caggcgtgta ttaatcacta cgacgacaca tatgtttatg ccaacatctc
    34981 actggcccgt ggttttctgt cgtgatcccg ccatgcttcc tcatgcgtct tttatatccc
    35041 ccatttcatt ttgttttcac tgctggaaag cgaaccaggg aaaagtgcag ggatttacgc
    35101 cagaagcgat tgatgcactg gtacaacgac cagagtgtga cgtaattctc attgaggcag
    35161 atggctcgcg tggaatgccg ttaaaagcgc ctgatgagca cgaaccttgc atacctaaaa
    35221 gcagttgctg cgtgattgct gtgatgggag gacatatttt gggtgcgaaa gtgagcacag
    35281 aaaatgtcca tcgctggtcg cagtttgctg atattactgg gttaacacct gatgcaccct
    35341 tgcaactgag cgatctcgtt gcgctggttc gccaccctca gggcgcgttt aaaaacgtac
    35401 cgcaaggttg tcggcgagtc tggttcatta accgtttttc tcaatgtgag aatgcgattg
    35461 cgcaaagcga gctccttcaa ccgctgcaac aacacaacgt agaggcaatc tggctgggcg
    35521 atatacaaga acatcctgca atcgcgcgca gatttgtgaa ttagcggccc gggtattccg
    35581 ggttactgaa aggatatcca ttgaggttgg tatgaatatt ttcacagagg ctgcaaaact
    35641 cgaagagcaa aattgtccgt ttgcgatggc gcaaattgtt gatagccgag gctcaacccc
    35701 ccgccattct gcacaaatgt tagtgcgcgc cgatggttct atcgtcggta caattggtgg
    35761 cggaatggtt gagcggaagg tgattgaaga gtcgcttcag gcattgcagg aacgtaagcc
    35821 gcgattattc catggacgta tggctcgtaa cggtgcggat gctgtcgggt cagattgtgg
    35881 aggtgcaatg tcagtattta tcagcgtcca tggtatgcgt ccacgtctgg tgttgattgg
    35941 tgcggggcat gtcaaccggg cgatagccca gagtgcggcg ctattaggat ttgatatcgc
    36001 cgttgccgat atttatcgcg aaagcctcaa tcctgaacta ttcccaccat caaccacgct
    36061 tctccatgct gagtcgtttg gtgcggcagt ggaagcactg gatattcgcc ctgataattt
    36121 tgtcctgatt gccacgaata atcaggatcg tgaagccctc gacaaactca ttgaacaacc
    36181 cattgcatgg ctggggttgc tggcaagtcg ccgcaaggtt cagcttttcc tacaccaatt
    36241 gcgtgagaaa ggcgtggctg aagaacatat tgcccgttta catgcgcccg ttggttacaa
    36301 cataggtgcg gaaacgccgc aggagatcgc catcagcgtg ctggcagaaa tattacaggt
    36361 gaaaaataac gcgccgggtg ggctgatgat gaagccttct catccttccg gacaccagct
    36421 ggtggtgatt cgcggtgcgg gggatatcgc cagtggtgta gcgctacgtc tgtatcatgc
    36481 gggttttaaa gtgatcatgt tggaagtgga aaaaccgaca gtgattcgtt gtaccgtggc
    36541 gtttgcccag gccgtgttcg atggcgaaat gacggtcgaa ggcgtcaccg cccgcctggc
    36601 aaccagctct gcggaagcga tgaaacttac cgaacgcgga tttatccctg tgatggtaga
    36661 tcccacctgt tcattgcttg atgaactgaa accgctttgc gtggtggacg ctattctggc
    36721 gaaacagaat ttgggaacgc gggcagatat ggcaccagta acaatcgcgc ttgggccggg
    36781 ctttactgca gggaaggatt gtcatgcggt aattgaaaca aatcgcgggc actggctcgg
    36841 tcaggtgatt tactcaggtt gtgcgcagga gaataccggt gttcccggca atattatggg
    36901 gcataccacc cgacgagtga tccgcgctcc tgctgcaggc attatgcgat ccaacgtgaa
    36961 attaggcgat ctggtgaaag agggcgatgt cattgcctgg attggtgagc atgaaattaa
    37021 agcaccgttg acggggatgg tgcgtggctt gttgaacgac ggactggccg tggtcggtgg
    37081 ttttaaaatt ggtgatatcg atcctcgtgg tgaaacggct gatttcacca gcgtttctga
    37141 taaagcccgg gcgattggcg gcggcgtact tgaggcgtta atgatgttga tgcatcaggg
    37201 cgttaaagcg acaaaagaag tgctggaagt ggcttaaatg aaaatggccg gatggtatta
    37261 cccatccggc ctttaaatta ctgacaaatc accgttccgg ttttgccttc aatcccttct
    37321 ttcgctttgc tcaatacggt aatcagtgct tcacgacctg cgcgagagcg agcaaacgag
    37381 gcggcagctt ccactttagg cagcatagaa cctttcgcaa aatgaccttc ttcgataaag
    37441 cgttcagcat cacttaatga caggcgatcg agccactgtt cattctcttt tccgaagtta
    37501 atggccactt tttctaccgc agtcagaatg atcagcatat cggcatcgat catttctgct
    37561 aaacgggcac tggcccagtc tttatcgata accgcgctgg caccacgcag atggttacct
    37621 tcacgaataa cagggatacc accaccgcca acggtgatca ccacctgtcc ggcatctacc
    37681 agagctttaa ccgtttcttt ttcaatgata tcaaccggtt ttggcgaagc aacgacacga
    37741 cgatagccgc gacccgcatc ttctttcaga gtgtaaccct gttttgtcag tagctctgct
    37801 tcttgctcgg taaagaacga accaatcggc ttggtcgggt tgaggaatgc cggatcgtta
    37861 gcatcgactt cgacctgggt aaccagcgtt gctacaggtt tattaatgcc acgagaaagc
    37921 agttcttctc ttaaggcgtt ttgtagatcg taaccaatat aaccctggct taaggcaaca
    37981 cagacagaca tcggcagcat tggcgagtgc gcttcagttt ttgcggcagc ttcaaaggcc
    38041 tggttaatca tgcccacctg tggcccgttg ccatgagtga cgatgacctc atgtccctgg
    38101 gcaattaaat caacaattgc ctgagaagta atttttaccg ctttcatttg tccggccagg
    38161 tcatcgccca gcgcattccc gcccagggcg agaacaattt tcttactcat ttttcctcac
    38221 tcatcattct tgattgtgta tagatccctg gcagtggtta gagcacggga gggacaaacg
    38281 gtttgcgtcg caggaaacga cctcgcccgg ctttgcctgt aaaggtgccg tcgcagaaaa
    38341 tcgtttcacc acgggataag gttctgacaa tcgcgccctg acaggtaaaa ccctcccatg
    38401 gcgagtagtc agcgttgtcg tggagatggc gatgctgaat ttgttggctc tgacgtgggt
    38461 ctataatcac cacgtcgcca tcggaaccgg gcgctaataa tcctttttgt ggccacaggc
    38521 caaacagcct ggcgggcatt gcactggtta actcaacaaa acgctccggt gagatacgcc
    38581 ccgtcatcac gccactggag aacagcagtt gcatgcggtt ctccacaccg ggtaagccat
    38641 ttgggcagcg gctgaaatcg cctttagaaa tttgcaggcg ttgagccatc gagaaggtgc
    38701 agtgatcggt tgccaccacg tcaatcgcac catcgctgat gccacaccac agtttgtcct
    38761 gctcgcgtac gttacgcagc ggtgggctaa gaatgaactt catgccatct tctgtatcgt
    38821 aactgcgttc gtccaacagg agatattgtg ggcaggtttc aacccagact ggctggtgat
    38881 tcgcacgggc aagacgcaga taatccagac ctaagccgtt agacaggtgc acgatataga
    38941 gcggggcgtt accggcaatt tgtgccaggt taatcatgcg ggcgatggct tccgcttcgc
    39001 attccagagg tcgactcaag gcatgataac gcggcgcggt taaccctgcg gcgataaact
    39061 ccgcccgctt gctggcgata gctgcgtcat tttccgggtg cacggtggtc agcgcgccgg
    39121 actcatgcaa gcggcgtaat gcctgcaaca cttcatcatc gttgagttta tattgatagg
    39181 ttaaatagag tttaaaactg ctcagtcctt cctcaaccat catcggaatt tcgtcgagga
    39241 tagcgtgatt aatatgttga atgacaccgt gaaagctgta gtcgatgact gctttgtggg
    39301 cggcataacc gcgataaact tccagttgat ggcgtaaccg gcagccattt gggccaaatc
    39361 ccatatggtc aataatggtt gttgtaccgc cacacgcagc tgcgcgggta ccggtaaaaa
    39421 aatcatcaca actgcgcgcg atgccgacat caatattgaa atgcgtatgg acatccacgc
    39481 cgccagggaa aacgtaacag ccagaggcat caatttcttc gcaagggagt tgcggcgaaa
    39541 tatcggtacc caactggcga acaatcccgc tttcaatcag caaatcctgc ttcgcctgtc
    39601 catctgcatt aacaacaatg ccgtttttta tcaatacgcg catagcaaac tccaaaaacc
    39661 ctccggtgaa ccggagggga ataagtgaag agtaattatt cggttgccag ccagcttaat
    39721 gggattgcag cgtacatagc ggcacaggtt accaggtgag atttccaggt tttttcgttt
    39781 ggcgcgtgcg cttccggttc tttaccgggg ccaaaaccga tgaccggaat gccgtggcga
    39841 cccatgatgg atacgccatt agtcgagaag gtccacttat caacgacagg cgctttacca
    39901 aacagccctt cataagcatt caccagtgct ttaacggtga agtgatcttc ttccactttc
    39961 caggtcggga agtagcattc ggttgggtaa accaggccag tccaggacgg gcggtcgtag
    40021 ttgtacatag aaacgacagc attcgctttt tgtactgcag gcagggcacg gatctcgtcc
    40081 agcgcgcctt cccaggtttc gccccaggtc agacgacggt cgattgaaac tgcgcagctg
    40141 tctgcgacag cgcaacggct tggggaggtg aagaaaattt cagaaacggt gagggtgcct
    40201 ttgccgagga attcgtcata acccagacgt tgggagagtt cttgtaattc gccaagaatc
    40261 ggacccattt tgaaaatggc gttatcaccg cgttctggtg ccgaaccgtg gcagctaaca
    40321 ccctgaacat caatacgaat ttccatacga ccgcgttgac cacggtatac ctggcagtcg
    40381 gttggttcgg tactgaccac aaattccggg cgaatgccgg attgttcaat aatgtactgc
    40441 cagcacaggc cgtcgcagtc ttcttcctgc acagtaccgg taaccagcag ggtatattca
    40501 tcttccagac cgagatcttt aatgatttta ccggcatata ccatagacgc catgccgcct
    40561 tcctggtcgg aagtaccacg accaccgatc agctcgtcgg tttccatgcc ttcatacgga
    40621 tcgaagtccc agtttttgat gttgccaatg ccgactgtat cgatatgagc gtccattgcc
    40681 accagacgcg ggccgtggcc gatataaccg agaacgttgc ccattgggtc gatttcaact
    40741 ttatcgaagc cgactttttc catctcttct ttaatacgat gtactacgcg tttctcgtcg
    40801 cagctttcac tgggaatagc aaccatatcg cgcaggaagc gagtcatgtc tgcctggtaa
    40861 tcttttgctt tttcaagaat cagtttgaat ggaatattct tagccattat ttttctccag
    40921 attttttact ccccgttttg cagggagcag ttccaatagg gtgattaagg tgctacagcg
    40981 tgtttgcctt cccagacaac ttcgcggtag tgcttcacgt cggtgtcgcc ttcagtgctg
    41041 ataaccagca ccacggcatc tttgttcagc gccagttttt ccatcaggct ttgacgttgc
    41101 gggtgataat gaaccgctgc gagaacgccc aaaccgacag cgccggattc accggagatg
    41161 atgcgcgggt cgttgccgta cggattaccc agcacgcgca tacctaatgc ggcaacgctg
    41221 tcctggcagg agatgaattg ggtggcgcag ttacgtagga tttcccagcc cagcgggtta
    41281 ggttcgccgc aggccaggcc cgccatgatg gtggccatat caccgccaac gttgacgatg
    41341 tcgcctttga cgccggagcg ataaatacag tcagctttgt caggttcaac aataatgctg
    41401 tgcagatttt gcgggctata gacgtcgacc agataaccca gcacaccacc ggccattgca
    41461 ccgacacctg cttgcagcag aacgtgtgtc ggggttacgc ccatttcacg catttgctcg
    41521 acggcttcat ccgccagtgt tgcgtagcct tgcatgatcc aggttgggat tttggtgtaa
    41581 ccttcccatg ccgtgtcctg taccacttcc cagccgtgct gctgcgcgtg ttgcatggtc
    41641 aggcgaacgg tatcgtcata gttcatatcc gtgacgatgc actcggcacc gaggtttaga
    41701 atggcgtcaa cgcgttcctg agcagaacct ttcggcatgt aaatcaccgc attctgtccg
    41761 agttgctgag cggcccatgc cacaccgcgc ccgtggttgc cgtcggtggt ggtcgcgaaa
    41821 gtcatttttt cgccaatggc atttttcagg tgctcaaatg atagcgtttc gatatcaaga
    41881 tgatattttt cacacaataa ttgagcgatg gcgtatgcgc cgccgagcat tttgaaggcg
    41941 ttcagaccga agcgttttga ttcgtcctta acgagaattt tctttacacc aaacaggttt
    42001 gcgagatcgt ccagcgcaca aagcggtgtt gggcgataac cagcaatttt ctggtggaac
    42061 tggcgtgcca gttttgcctg gctttgcgaa aaaagcggtg atgtttcgcc gttgaaaaat
    42121 ttgttatcgg cgatatcaat ctttaatgag aaaacggaca tatcctatcc ttttggcctc
    42181 tggaaataga gggccgacca gctgatcggc ccgaggggtt atttcacgcg ttcttgcgct
    42241 tctttcagca gttgctcgag cagtgcacct ggtttggcgt atttacggga caggatcatc
    42301 gcggcgatga tataaggctt ccagctggct tctttgtagg tagcgatacg gtatttttcg
    42361 aatacgcctt cagtcacttc accttctttg caggatacgc cgctgatatc agctggcagg
    42421 cagtgcatgt acagggcttc gccatcacgg gtcagctcca tcatctcttc ggtacaatgc
    42481 cagtctttgt gctgtgcgtt ctgtgccaga cactgttttt ccagcgcttt cagtccttcg
    42541 tgatcgttcg cacgcagcag ttcagtacgc tcttccatca ctttgtaagg tgcccaggac
    42601 ttcggataaa cgatgtctgc gtctttgaag gcttcttcca tgctggtgac ctgacggaag
    42661 ctaccaccgg aggctttagc attgttttta gcaacttcaa cgacatccgg gatcaggtcg
    42721 tagccttccg gatgggccag ggtgacatcc ataccgaagc gggtcatcag accgatgatg
    42781 ccttgtggta cagagagcgg tttgccatag cttggggagt aggcccaggt catggcgatt
    42841 tttttacctt tcaggttttc cagtgaacca aagtgttcac gtaaccatgc cagatcagcc
    42901 attgattgag tcgggtggtc aatatcgcat tgcaggttca ctaaagccgg acgctgcggc
    42961 agcacaccct gcttgtaacc gtcatcaagt gcagcgccaa cttcacgcat ataggcgttg
    43021 cctgcgccca gatacatatc gtcgcgaata ccgatagcgt cagcgcagaa ggagatcata
    43081 ttggcggttt cacgcacggt ttcgccgtga gcgatttgtg atttaccttc gtcaagatct
    43141 tgttgtgcaa ggccgagcag gttaagcgcg gaagcataag agaagcgggt acgggtggaa
    43201 ttgtcgcgga atacggaaat acctaatcca ctattaaaga ctttggttga gatgttttca
    43261 gcacgcagtg cttttaacgc ggcggcaacg tccagtactt gtttcagttc gtctggcgtc
    43321 tgttcccacg ttaacaaaaa atctttttca tgcaggtgag aggtcagcga attgatatcc
    43381 ttaatcagct cattaacagt tttcatcata aagtcccgat agtggaatga caggcaaagt
    43441 ggtttatttc actgtgacgt gccaaagtga ttggtgtttt cacagctata cctcggacta
    43501 acaataagtg tgccagaatt atccacgcgg ctgcgaaagg ctaatttgac ggataatgtg
    43561 tggggttgat cacatattga tgccagtttc cggttgtttg taaaatacga aaacgtttgg
    43621 ctttttttat tacgttattt atttgctgct aagtatcaga aataag
//
LOCUS       DACADF010000021        41577 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.021, whole genome shotgun sequence.
ACCESSION   DACADF010000021 DACADF010000000
VERSION     DACADF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.021"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(136..4017)
                     /gene="entF"
                     /locus_tag="ISB30_001122"
     CDS             complement(136..4017)
                     /gene="entF"
                     /locus_tag="ISB30_001122"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HAP1988777.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHPLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQI
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTRLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCSTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRVP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLFEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVAFDGKATLFVAERTLQEGMSPEQAWAPWIAEL
                     DIYRQDCAHVDIISPEYFKEIGPLINTQINN"
     gene            complement(4014..4232)
                     /locus_tag="ISB30_001123"
     CDS             complement(4014..4232)
                     /locus_tag="ISB30_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HAP1988778.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(4235..5437)
                     /gene="fes"
                     /locus_tag="ISB30_001124"
     CDS             complement(4235..5437)
                     /gene="fes"
                     /locus_tag="ISB30_001124"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HAP1988779.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEGHS
                     AIKRVWVYITGMTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTIGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGMLLEQLKTGEVSAEG
                     LRIVLEAGVREPMIMQANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5680..7920
                     /gene="fepA"
                     /locus_tag="ISB30_001125"
     CDS             5680..7920
                     /gene="fepA"
                     /locus_tag="ISB30_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HAP1988780.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     KNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            8095..8754
                     /gene="entD"
                     /locus_tag="ISB30_001126"
     CDS             8095..8754
                     /gene="entD"
                     /locus_tag="ISB30_001126"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HAP1988781.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPTGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKACER
                     QTDAGFLRYQIRNCQHQQMNISGPNGDHIVQWLVRGNNVITLCLADKHPHTAACGLSG
                     G"
     gene            complement(8756..9691)
                     /locus_tag="ISB30_001127"
     CDS             complement(8756..9691)
                     /locus_tag="ISB30_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003831178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HAP1988782.1"
                     /translation="MRNSEHLANVMVQAFIDAVNEGIDLVPVVADSTSTAKIAPFIKQ
                     FPDRLVNVGIAEQSMVGTAAGLALGGKVAVTCNAAPFLIARANEQIKVDVCYNFTNVK
                     LFGLNAGASYGPLASTHHAIDDIAVMRGFGNIQIFAPSSPRECRQVIDYALRYQGPVY
                     IRMDGKALPEIHDEDYRFVPGAVVTLREGSELALVATGSTVHEVVDAAELLAQSGIAA
                     TVVSVPSIRPCDTKALLAALKGCKAVMTVEEHNVNGGLGSLVAEVLAEAGTGITLKRA
                     GIMDGEYAAAADRGWLRQHHGFDAAGIAAQAREML"
     gene            complement(9684..10514)
                     /locus_tag="ISB30_001128"
     CDS             complement(9684..10514)
                     /locus_tag="ISB30_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP1988783.1"
                     /translation="MNPYSYDIQTLERKARAVRRHIVRLNANSPAGGHTGADLSQVEL
                     LTALYFRILNVAPDRLHDDDRDIYIQSKGHAVGCYYCVLAEAGFFPLEWLSTYQQPNS
                     HLPGHPVRQKTPGIELNTGALGHGFPVAVGLALAAKKSQSRRRIFLITGDGELAEGSN
                     WEAALAAAHYGLDNLVIINDKNNLQLAGPTREIMNTDPLAEKWLAFGMQVTECQGNDM
                     ASVVATLEELKQEGKPNVVIAHTTKGAGISFIQGRAEWHHRVPKGDEIEQALEELKDA
                     "
     gene            10877..11827
                     /locus_tag="ISB30_001129"
     CDS             10877..11827
                     /locus_tag="ISB30_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAP1988784.1"
                     /translation="MAKQDEQRLLVKIATLYYLEGRKQSDIAQLLSLSQSFISRALTR
                     CQKEGVVKISVVQPSNIFLNLEKGIEERYGIKQAIVVDTEDDASDHTIKRAIGSAAAH
                     YLETRLRPKDFIGVSSWSSTIRAMVDEVHAQNLKASGVIQLLGGVGPNGNVQATILTQ
                     TLAQHLNCEAWLLPSQSIEGSVEEKKRLVASQDVADVIARFDDVDIAIVGIGILEPSQ
                     LLKTSGNYYHEDMLQVLADRGAVGDICLHYYDKHGQPVLQDDEDPVIGMALDKIKKCP
                     NVVALAGGKDKVAAIKGAMQGGYIDVLITDFPTARMLIAD"
     gene            12178..13698
                     /locus_tag="ISB30_001130"
     CDS             12178..13698
                     /locus_tag="ISB30_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAP1988785.1"
                     /translation="MFAGNKEFVIALDEGTTNAKAIALDGFGNVVAKFSQPLAIQTPR
                     EGWVEQSGEALISASLDVIAQAINHVGAENVAALAISNQRETAIGWYRQSGKPLNAAI
                     TWQCTRSAEFCETLRREGKEQQIKTTTGLPIAPLFSASKMRWLLESTPEGVQLADQGE
                     ICLGTIDAWLLWNLTAGDSFCCDYSNAARTQLLNLKTGEWDEEMLALFHIPRAALPEI
                     KPSSGLFGYTRGLKTIPDGIPVMAMVGDSHAALFGHALGEAGCVKATYGTGSSVMAPV
                     NSAQCDIATLATTIAWHDGENIIYGLEGNIPHTGDAVAWMADSTGLSELTDAELALEL
                     NTLPASVDSTMGVYFVPALTGLGAPWWDDSARGVVCGLSRGVKRAHLIRAALESITYQ
                     IADVVEAMQQHDDFHLSALMVDGGPTKNDWLMQYQADLLGCPVMCSDVPELSAIGAGL
                     LARKALIPGTVADLKALLTEHSEFKPDMARHQRLQKRWQEWRHAVNRTLWKPASSI"
     gene            13802..14737
                     /locus_tag="ISB30_001131"
     CDS             13802..14737
                     /locus_tag="ISB30_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP1988786.1"
                     /translation="MKLRLTLLTAATLTALSFSAHAVEKGTIMIMVNSLDNPYYASEA
                     KGASEKAQQLGYKTTILSHGEDVKKQNELIDTAIGKKVQGIILDNADSTASVAAIEKA
                     KKAGIPVILINREIPVDDVALVQITHNNFQAGSEVANAFVEKMGEKGKYAELTCNLAD
                     NNCVTRSKSFHQVIDQFPEMVSVAKQDAKGTLIDGKRIMDSILQAHPDVKGVICGNGP
                     VALGAIAALKAANRNDVIVVGIDGSNDERDAVKAESLQATVMLQAQAIAAQGVTDLDN
                     YLQKGVKPAKQRVMFRGILITKDNADKVQDFNIKS"
     gene            14784..15404
                     /locus_tag="ISB30_001132"
     CDS             14784..15404
                     /locus_tag="ISB30_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 domain-containing protein"
                     /protein_id="HAP1988787.1"
                     /translation="MDKKVWLALAILSLGGCRIVSQQELADLKSPPNPHMANIDQTWQ
                     KNIVPQVVENARPVAELMAALQAEKDVDAACKTLGYRSQEENPCIFYVKVEGSITNID
                     AKSRSGKMTITDISGTNIVVQTGPTLRGTLLRDAYKGASYEHFNDQVLFGEYGRAINE
                     QAINMIQSAKLKTGETVQVYGVFSAWDIPQTIPDITPAKIARAGGE"
     gene            15406..16950
                     /locus_tag="ISB30_001133"
     CDS             15406..16950
                     /locus_tag="ISB30_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP1988788.1"
                     /translation="MLPQEIAPPQTDESPVIIETHDLSRIYPGVVALDNVNYRVYRNK
                     VNVLIGENGAGKSTMMKMLAGVETPSSGKIILDGKEVTLNSTHQAEKHGISIIFQELN
                     LFPNMNVMDNIFMANEFFQKGKINEKYQYELAKSLLERLELNVDPYTQLGELGIGHQQ
                     LVEIARALSKDTRVLIMDEPTSALSQSEVKVLFNVIAQLKRRGVTIIYISHRLEELME
                     IGDFITIFRDGRFISERAVSDASIPWIIEQMVGDKKKHFDYQPATQGDTVLAVQGLTA
                     LHPSGGYKLNDVTFSLHKGEVIGIYGLLGAGRTELFKGLVGLMPCQSGKIQLNGETID
                     KCHFQARLKKGLALVPEDRQGEGVVQMMSIQSNMTLSDFSLQGFRRAWKWLNPQKESA
                     SVKEMILQLAIKVSDAELPITSLSGGNQQKVVLGKALMTQPQVVFLDEPTRGIDVGAK
                     TDVYQLVGKMAQQGLAVMFSSSELDEVMALADRILVMADGRITADLPRGEVTREKLIA
                     ASTPQD"
     gene            16968..18035
                     /locus_tag="ISB30_001134"
     CDS             16968..18035
                     /locus_tag="ISB30_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1988789.1"
                     /translation="MNQKYMIYMYLLKARTFIALLLVIAFFSVMVPNFLTASNLLIMT
                     QHVAITGLLAIGMTLVILTGGIDLSVGAVAGICGMVAGALLTNGLPLWSGNIIFFNVP
                     EVILCVAVFGILVGLVNGAVITRFGVAPFICTLGMMYVARGSALLFNDGSTYPNLNGM
                     ETLGNTGFATLGSGTFLGIYLPIWLMIGFLILGYWITTKTPLGRYIYAIGGNESAARL
                     AGVPIVKVKVFVYAFSGLCAAFVGLIVASQLQTAHPMTGNMFEMDAIGATVLGGTALA
                     GGRGRVSGSIIGAFVIVFLADGMVMMGVSDFWQMVIKGLVIVTAVVVDQFQQKLQNKV
                     ILMRRHEEKMAAAPAVKPLSS"
     gene            18089..18886
                     /locus_tag="ISB30_001135"
     CDS             18089..18886
                     /locus_tag="ISB30_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1988790.1"
                     /translation="MQRNFQGKTVVITGACRGIGAGIAERFARDGANLVMVSNAERVH
                     ETAEQLRQCYQADILSLEVDVTDEAQVQALYEQAAARFGSIDVSIQNAGIITIDYFDR
                     MPAADFEKVLAVNTTAVWLCCREAAKYMVKQNHGCLINTSSGQGRQGFIYTPHYAASK
                     MGVIGITQSLVHELAQWNITVNAFCPGIIESEMWDYNDRVWGEILSTDDKRYGKGELM
                     AEWVEGIPMKRAGKPEDVAGLVAFLASDDARYLTGQTINIDGGLIMS"
     gene            18919..19704
                     /locus_tag="ISB30_001136"
     CDS             18919..19704
                     /locus_tag="ISB30_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1988791.1"
                     /translation="MIAKDFAQQLFDLHDRVAFVTGAGSGIGQMIALALASAGARVVC
                     FDLREDGGLKETVNHIEALGGQALYYTGDVRELRDLRAAVALAKTHFGRLDIAVNAAG
                     IANANPALEMESEQWQRVIDINLTGVWNSCKAEAELMVENGDGSIINIASMSGIIVNR
                     GLEQAHYNSSKAGVIHLSKSLAMEWVSKGIRVNSISPGYTATPMNTRPEMVHQTREFE
                     SQTPIQRMAKVEEMAGPALFLASDAASFCTGVDLVVDGGFVCW"
     gene            complement(19814..19966)
                     /locus_tag="ISB30_001137"
     CDS             complement(19814..19966)
                     /locus_tag="ISB30_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP1988792.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(20316..20468)
                     /locus_tag="ISB30_001138"
     CDS             complement(20316..20468)
                     /locus_tag="ISB30_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP1988793.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            20911..22029
                     /locus_tag="ISB30_001139"
     CDS             20911..22029
                     /locus_tag="ISB30_001139"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAP1988794.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDTVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FISLSAASPYMQGTDTRFASSRPNIFSAFPDNGPMQWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEATLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGE"
     gene            22095..22343
                     /locus_tag="ISB30_001140"
     CDS             22095..22343
                     /locus_tag="ISB30_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAP1988795.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            22408..22791
                     /locus_tag="ISB30_001141"
     CDS             22408..22791
                     /locus_tag="ISB30_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAP1988796.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPDSTVNQ"
     gene            22870..23523
                     /gene="nfsB"
                     /locus_tag="ISB30_001142"
     CDS             22870..23523
                     /gene="nfsB"
                     /locus_tag="ISB30_001142"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAP1988797.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            23631..24878
                     /locus_tag="ISB30_001143"
     CDS             23631..24878
                     /locus_tag="ISB30_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP1988798.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(25036..26418)
                     /gene="pheP"
                     /locus_tag="ISB30_001144"
     CDS             complement(25036..26418)
                     /gene="pheP"
                     /locus_tag="ISB30_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAP1988799.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRKVN"
     gene            complement(26520..29663)
                     /gene="cusA"
                     /locus_tag="ISB30_001145"
     CDS             complement(26520..29663)
                     /gene="cusA"
                     /locus_tag="ISB30_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAP1988800.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVSDPQRLAQYGISLADVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKNDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(29675..30898)
                     /gene="cusB"
                     /locus_tag="ISB30_001146"
     CDS             complement(29675..30898)
                     /gene="cusB"
                     /locus_tag="ISB30_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HAP1988801.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKEEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFSQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEIDNADEALKPGMNAGLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(30914..31246)
                     /gene="cusF"
                     /locus_tag="ISB30_001147"
     CDS             complement(30914..31246)
                     /gene="cusF"
                     /locus_tag="ISB30_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HAP1988802.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(31270..32652)
                     /gene="cusC"
                     /locus_tag="ISB30_001148"
     CDS             complement(31270..32652)
                     /gene="cusC"
                     /locus_tag="ISB30_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAP1988803.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTRELSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDITEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            32809..33492
                     /gene="cusR"
                     /locus_tag="ISB30_001149"
     CDS             32809..33492
                     /gene="cusR"
                     /locus_tag="ISB30_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAP1988804.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            33482..34924
                     /gene="cusS"
                     /locus_tag="ISB30_001150"
     CDS             33482..34924
                     /gene="cusS"
                     /locus_tag="ISB30_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAP1988805.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLANSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIILPA"
     gene            35074..37311
                     /gene="nrfB"
                     /locus_tag="ISB30_001151"
     CDS             35074..37311
                     /gene="nrfB"
                     /locus_tag="ISB30_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HAP1988806.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            37298..40270
                     /gene="nfrA"
                     /locus_tag="ISB30_001152"
     CDS             37298..40270
                     /gene="nfrA"
                     /locus_tag="ISB30_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HAP1988807.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDDRLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAAQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPASLTRLDQLTWQLMQN
                     GQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPDAWQHRAVAYQAYQVEDYATALTAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNIPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WSFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRLGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            40271..41161
                     /locus_tag="ISB30_001153"
     CDS             40271..41161
                     /locus_tag="ISB30_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAP1988808.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRATAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISIFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
BASE COUNT        10211 a        10907 c        10585 g         9874 t
ORIGIN      
        1 ataacaaact tagcaacccc agcaatgacg ctgctctcag aaaaataaat aaattttaag
       61 aataaaaaaa ccataatcat ttagtcggat tatggtttta aatgaggtta tggcaagttc
      121 tttactaacg tacggttagt tattaatttg tgtatttatt agtggtccta tttctttaaa
      181 atactccggg gaaataatat ccacatgcgc acaatcctga cgatagatat ccagctcagc
      241 aatccacggt gcccaggcct gttcaggact cataccttcc tgaagcgtgc gttcggcaac
      301 aaatagcgtc gctttaccat caaacgctac gctatgagct gtcgtcagca ggcgcacagc
      361 atcagcgtag ttgccttcaa tggtggtaaa caactccgtt gaagtacttc cctgctgtgc
      421 cgccaggaag gcttcgcgct cgcggttaat ctccgccagc acttccgggt ccagaccatt
      481 ggcttctttt tcctgccagt tttgcgtttc tggcggccag gtatccagca agccaagaaa
      541 tgccacctgt tcgccacggg cacgcagtcg cgccgcaatg ccctgcgcca gcgtaccgcc
      601 aagtgaataa cccagcaggt aataagggcc gtgcggttgt tgttcaaata acgttgccag
      661 atgcgcttcg cagacttcat ccaggtttgc cgccgtctgc atggggccat gagggcgcgg
      721 cgactgaatg ccgataatcg accattgtgg atcgagataa cgcgagagca cgctgaactg
      781 ccaggcaaaa ccggatgccg gatggaaaca aaacagcgtc gggccattac cttcacgcaa
      841 cggcagaatg gtttcgaatc ccatgcgtcg ggtgctgtct tcttcaccat caataatcgt
      901 tgccagtttg gcgacagttg acgcgaccat cacctgcccc ggcgtcacct gacgggcaaa
      961 ctgtcgactt aactgcgctg ccagtttcat tgccagtagc gaatgaccgc caagcgcaaa
     1021 gaaatcagca tcggcatcct gcacgtcaca acccagcaac gacgagaatg ccgcggcgat
     1081 aatcgtttca ctgcccgctt tcggcacacg ccccggcgtt tgtgccttca gttcaggcaa
     1141 cggtaaggct ttgcgatcca gcttgccgtt ggcgctaagt ggtaactgtg gaagttgcag
     1201 cagaaccacc ggtaccatat gtggtggcaa tgtttctcga agctgcgcct gtaatgcgct
     1261 ggtatccaac ggcagacctg attgcgacac cagatagccc accaactgac gcgcatcacc
     1321 accagtggct gccgcctggt taatcacaca ggcgtgggta acggcttgtt cgacatccgg
     1381 cagcgcctgc atcacgcgat caatttcgcc cagttcgata cgctgcccgc gaatttttag
     1441 ctgatcgtca ctgcgcccga ggtactccac tgcgccgtta tccagccagc gggcaacgtc
     1501 tccggtacgg tacatccgtt cacctggggc aaaaggatcg gcaataaagc ggctggcggt
     1561 cagatcgggt cgtccaagat acccctgcgc cagttgaata ccggtgagat agagatcgcc
     1621 cgccacaccc ggcggcaccg gatgcatcat cgcatcgaga atgcgcaggc ccgtattcca
     1681 caccggataa ccaatcggca cactgctgcc gcgcacctct gccagttcct cgccaaaagc
     1741 cggataccaa ctgacatcta ccgccgcttc cgtcgggcca tatagattat gcaacggcgc
     1801 gccggttaac tgttgccatt cgcggcataa atcagccggt agggcttcac cactacagaa
     1861 aacctgtttc aacgtcgagc aattctgacg agcggtttgc ggcgtcagcg atgcaacaaa
     1921 tgctgccagc atcgacggca caaagtgcgt ggtcgttaca ccatattcgg caaagaattg
     1981 ctgcatagcg agcgggtcgc ggtgcgcttc cggttcagcc atcaccagtt tagccccggc
     2041 aataaacggc cagaaaaact cccacaccga gacatcaaaa ctgcacggcg ttttttgggc
     2101 aacgacatct tcgcctgtaa gcgggtagtg gttttgcatc caaagcaggc ggttaacgat
     2161 agccgtctgc ccgaccatta cccctttcgg cctgccggtg gagccagagg taaagatgat
     2221 ataagccgtg tggtgcggtt gtgaaagctg cagcggcgca ctgccctgcg gtgtaagcgg
     2281 ggcgttatag caaaggcggg ttaaatctgg aacatcggca aagcgcggca gttgatcgtc
     2341 agtggtgatt aacagcgacg gacgcgcatc ttccagcatc attttcaggc gatcgtccgg
     2401 atagccggta tccagcggta accaggccgc accagcctca acaattgcat gtagcgcgag
     2461 agtcaaaaag accgagcgcg gtagagccac cgccacgctg tcgcccggtt taacgccgcg
     2521 ctcacgcagc agattcgcca gcgccaccac ctgctcgcgc atttcccgat agctgaactg
     2581 gtaacgcgca tctgccagcg ccggagcatc cggtgttttt gccgcttgtt ctgccaccag
     2641 cgcgctcagc gtggtttctg gaatctcaat ctgagtggcg ttgatctgcg ccagctgcgc
     2701 atactcacct ggcagcataa tatcgacatc gccgcacaac agcgccggat ccgcggcgaa
     2761 ctgggcaatc agcattttca ggcgttcagc gtgttgaatt aacgttggct catcgtaacg
     2821 ctgtttattg gcgaggattt caatactcaa atctccgtgt tcatccggga acagggccag
     2881 ttcaaggtca ttaaccggac cggttgccag gggatgggtt tgcgcctgaa caccaggaat
     2941 atccagttgg taatcaaata ccttgatatt gagtaccgga ccgaacagcg gttcctctcc
     3001 tgccgctcgc ccactgtcac ggacaatttg ttcggcgtcg tagcgttgat ggcggcgcat
     3061 ttttttcagt tgtgctgcca gtcgggttgc cagttgcggc agcgtttctt gcgccgcaat
     3121 gtgaataccc aacggtaaaa cgttgagcac gggtccggta gccgtcagcg ccgccgagcc
     3181 cagtcgacgc ataaagataa atccggcagc gtagtccatg cggttgcaca atcgccccag
     3241 ccacaaggct gccaacgcaa gtgctaaatc ggtacgctgc acacctgaaa gttgcgtagc
     3301 cagctggcgg aattccccgt cggtaaattc cagtttcagg cgcaaaatat ctgccgaagc
     3361 gctgcgcccc ggtaaaggtg ccggagaaag tgacgcgggc ggtggcagtt gacgacgctg
     3421 ttccgcccag aatgccgcat cacgctgcca ggcttcactt tcgcggtact gctgatactc
     3481 ttccactaca tcagcgaaag gcgtaaacgg cgaagcaggc gttggttcgc cacgcagcaa
     3541 tgcgcagtaa atattggcga tctggcgtgt aatggccggg aaactgaagc catcgaccag
     3601 caaatggtga taacgctgat accagtacca gcggttatcc gccacctgta tcagctgatg
     3661 aaagaccagt ggtttaccac tatcgacgcg cagatcttgt tgcaaatccg cctgcattaa
     3721 tgcccgcgca gtaccgtgcg gatcaatgtt ggttcgcagg tcgataattt ctggcagttc
     3781 gaacgtctgc gcatcatcga cccactgcca gacttcgccg ttatcttccg taaaacgcat
     3841 ccgcaacgta tctgcttgcg ctagtccggc aaccaccgcg cgggccagta gtggcgcatc
     3901 aacctctccg gttaactcaa cgtaatgcgc cacgctccag gcggagggta aatccgacag
     3961 tttttctgcc atccagatgc cgggctgtgc ggcgaccaaa ggtaaatgct ggctcattgt
     4021 gcctcctgca actgggtgaa attcgccggt gtcagagtac gccagtgggc ttccagccac
     4081 tgctggcagg acgcctgtga ctgcggctga cacacaacgt cccagcctgc cggtaaggcg
     4141 cattgttgcg gccacagact gaattgcccc tgcgcattgc gcaaaatgta aaacgctccc
     4201 tgtggatcat cgaagggatt actgaatgcc atattcaact cctgtcatgg aaaagtggtt
     4261 gccagaggtc gattagcccc tgcatcaagc caccgcgcca acaaagcgca tcatgtccgc
     4321 cgtcaacctg acgccagaaa atggattctt ttagggggtg taattgcgca tacagcgcct
     4381 gattggcctg catgatcatc ggctcgcgaa cacccgcttc cagcacaatg cgcagacctt
     4441 cggcgctaac ttcaccagtt tttagctgtt caagtaacat gccctcttgg tgcccgcccc
     4501 gatgcggcca ccagtacgaa cctgactggc ttaatacaca gccaaagcgt tcaggccagt
     4561 gcagtccggc atacagcgcg gaaagcccac caaaactctg tccggcgacc acggtgcgat
     4621 cggcgcgatc gctaaaaggg gcaatagctt tcacctgggg taataactct tgctgtactg
     4681 ccagccagaa atccgcatta cacggcagtt cgtgggcgcg gtgcgtggtg tcgatagcgt
     4741 cgatcaacac atacacggcg ggcggaagtt gccgacgatg ggtcagcgaa gtcagcgcag
     4801 gccagacggg catactctgc gcccaaaatt cgccatcaag caaaactgcc agcgggcgtt
     4861 cttctgctgt tgcatcgccg atggtaaaaa tccatacacg gcgtgaattt ttcaaccgtt
     4921 cacttttcca gataatttct ttggcaggcg tttctggcgc ttgcggacaa tcccatcccg
     4981 gttgcagagg cgcttgcggc atttcgagtg cagaaacagc gtgccctcgc ccgcctttcc
     5041 agctttgtgg attcagcgga tcggctatcg cctggggtaa tagttttcgc cagccttcgc
     5101 gtaattcgag gcgatcgggg gatggtgcag aaaaaatgtc atcgcgttcg gtaggaataa
     5161 agcagtagct gccgcgccag ttggcattga gttgtgtcgt ccactgccaa acatcggttc
     5221 ctgcaattcg ctgcatcgac cggggctggc tgttctgatg gtgatcggtc atgccagtga
     5281 tatagaccca tacgcgcttt atcgccgagt ggccttcaga accttgtgga tcacgccacc
     5341 aaaaagtgac ttcaaacatt tcgtcattca gacgttgcca ttccgggcca tgtttcgact
     5401 gccaccagct ctcacttcct acttttaacg ccgtcacacc ataaccccat gtttactgtg
     5461 caatttttta ttgattgcag aaatatattg ataatattat tgataactat ttgcatttgc
     5521 aatagcgtaa tggcgcgccg tgggaagcgc ggacattaat taatcagctg cactgcgtgt
     5581 ctttcaggat caaaggtttt cgcggtagcg ggatgcgtcg tgttgatgac gaccatgccc
     5641 gacagttgca attcgtggca aaaatgcagg aataaaacaa tgaacaagaa gattcattcc
     5701 ctggccttgt tggtcaatct ggggatttat ggggtagcac aggcacaaga gccgaccgat
     5761 actcctgttt cacatgacga tactattgtt gttaccgccg ccgagcagaa cttgcaggcg
     5821 cccggcgttt cgaccattac cgcagatgaa atccgcaaaa acccggttgc ccgcgatgta
     5881 tcggagatca ttcgtaccat gcctggcgtt aacctgaccg gtaattccac cagtggtcag
     5941 cgtggtaata accgccagat tgatattcgc ggcatgggtc cggaaaacac gctgattttg
     6001 attgacggca agccggtaag cagccgtaac tctgtgcgtc agggctggcg tggcgagcgc
     6061 gatacccgtg gtgatacctc ctgggtgccg cctgaaatga ttgaacgtat tgaagttcta
     6121 cgtggtccgg cagctgcgcg ttatggcaac ggcgcggcgg gcggcgtggt taacatcatt
     6181 accaaaaaag gcagcggtga gtggcacggc tcctgggatg cttatttcaa tgcgccagaa
     6241 cataaagagg aaggtgccac caaacgcacc aacttcagcc tgaccggtcc gctgggcgac
     6301 gaattcagct tccgcttgta tggcaacctc gacaaaaccc aggctgacgc gtgggatatc
     6361 aaccagggcc atcagtccgc gcgtgccgga acgtatgcca cgacgttacc agccgggcgc
     6421 gaaggggtga tcaataaaga tattaacggc gtggtgcgct gggacttcgc gcctctgcaa
     6481 tcactcgaac tggaagcggg ctacagccgc cagggtaacc tgtatgcggg tgatacgcag
     6541 aacaccaact ctgacgctta cactcgctcg aaatatggcg atgaaaccaa ccgcctgtat
     6601 cgccagaact actcgctgac ctggaacggc ggctgggata acggcgtgac caccagcaac
     6661 tgggtgcagt acgaacacac ccgtaactcg cgtattccgg aaggtctggc gggcggtacc
     6721 gaagggaaat ttaacgaaaa agcggcacag gattttgtcg atatcgatct tgatgacgtg
     6781 atgctgcaca gcgaagttaa cctgccgatt gatttcctcg taaaccaaac gctgacgctg
     6841 ggtacagagt ggaatcagca acggatgaag gacttgagtt ccaacaccca ggcactcacc
     6901 ggaacgaata ccggcggtgc tattgatggt gtgagtgcca ccgaccgtag cccatattca
     6961 aaagcagaaa ttttctcgct gtttgccgaa aacaatatgg agctgactga cagcaccatc
     7021 gtaacgccgg ggctgcgttt cgatcatcac agtattgtcg gcaataactg gagcccggcg
     7081 ctgaacatat cgcaaggttt aggcgatgac ttcacgctga aaatgggcat cgcccgcgcc
     7141 tataaagcgc cgagcctgta ccagactaac ccaaactaca ttctctacag taaaggtcag
     7201 ggctgctatg ccagcgcggg cggctgctat ctgcaaggta acgatgacct gaaagcagaa
     7261 accagcatca acaaggagat tggtctggag ttcaaacgcg acggttggct ggcgggcgtg
     7321 acctggttcc gtaacgatta tcgcaataag attgaagcgg gctatgtagc tgtagggaaa
     7381 aacgcagtcg gcaccgatct ctatcagtgg gataacgtgc cgaaagcggt ggttgaaggt
     7441 ctggaaggat cgttaaacgt accggttagc gaaacggtga tgtggaccaa taacatcact
     7501 tatatgctga agagtgaaaa caaaaccacg ggcgaccgtt tgtcgatcat cccggagtat
     7561 acgttgaact caacgctgag ctggcaggca cgggaagatt tgtcgatgca aacgaccttc
     7621 acctggtacg gcaaacagca gccgaagaag tacaactata aaggtcagcc agcggttgga
     7681 ccggaaacca aagaaatcag tccgtacagc attgttggcc tgagcgcgac ctgggatgtg
     7741 accaagaatg tcagtctgac cggcggcgtg gacaacctgt tcgacaaacg tttgtggcgt
     7801 gcgggtaatg cccagaccac gggcgatctg gcaggagcca actatatcgc aggtgccggg
     7861 gcgtatacct ataacgagcc gggacgtacg tggtatatga gcattaatac tcacttctga
     7921 agctaacgtc agattgttga caaagtgcgc gtagttcatg ccggatgcgg cgtgaacgcc
     7981 ttatccggcc tacaaaaatc ttgccaattc aatatattgc aggaccacgt aggcctgata
     8041 agcgtagcgc atcaggcagt tttgcgtttg tcatcattct ctaatatggt tgacatgaaa
     8101 actacgcata cctccctccc ctttgccgga catacgctgc attttgttga gttcgatccg
     8161 gcgagttttc gcgagcagga tttactctgg ctgccgcact acgcacaact tcaacacgct
     8221 ggacgtaaac gtaaaacaga gcatttagcc gggcggatcg ctgcaattta tgcgctgcgg
     8281 gaatatggct ataaatgtgt gcctgcaatc ggcgagctac gccaacctgt gtggcctacg
     8341 ggggtatacg gcagcattag tcactgtggg actacggcat tagccgtggt atctcgtcaa
     8401 ccgattggca ttgatatcga agagattttc tctgtacaaa ccgcaagaga attgacagac
     8461 aacattatta caccagcaga acacgagcga ctcgcagaat gcggtttaac cttttctctg
     8521 gcgctgacac tggcattttc cgccaaagag agcgcattta aggcatgtga aagacaaact
     8581 gatgcaggat ttctgcgcta ccaaatcaga aattgtcagc atcaacagat gaatataagc
     8641 gggccgaatg gcgaccatat cgtgcagtgg ttagtaaggg gtaataatgt tattaccttg
     8701 tgcctggcag ataaacaccc gcacactgca gcatgcgggt tatctggcgg gtaattcaaa
     8761 gcatttcccg tgcctgagct gcaattccgg cagcatcaaa tccatgatgc tgacgcaacc
     8821 acccacgatc cgccgctgcg gcatattctc catccataat gcctgcgcgt tttaatgtga
     8881 tccccgttcc ggcctctgcc aacacttccg cgaccaggct accaaggccg ccattgacgt
     8941 tatgctcttc aaccgtcatc actgctttgc atccttttaa cgctgccagc aatgctttgg
     9001 tgtcgcatgg gcgaatcgac ggtacgctga caactgtagc cgcgatacca gactgcgcta
     9061 aaagttctgc ggcatcgacc acttcatgaa cggtagagcc tgtggctacc agcgccagtt
     9121 cgctgccctc gcgcaaagtc accacggcac ctggtacaaa tcggtagtcc tcatcgtgga
     9181 tttccggcag ggctttaccg tccatacgga tataaacagg cccctgatag cgaagagcat
     9241 aatcaatcac ctgacggcac tcccgcggtg aagacggggc aaagatttga atattgccga
     9301 aaccacgcat aaccgcaatg tcatcaatcg catgatgcgt gctcgccagc ggtccataac
     9361 tcgccccggc attcagaccg aacaatttta cgttggtaaa gttgtagcag acatcaacct
     9421 tgatttgctc gttagcgcga gcgataagaa acggcgcggc attgcaggta acagccacct
     9481 tgccgcccag cgccagcccg gcagctgtac caaccatgct ctgttcggca atacccacat
     9541 tcaccagtcg gtcagggaat tgcttaataa agggagcaat ttttgccgta gacgttgaat
     9601 cagccaccac aggcaccaaa tcgatcccct catttacggc atcgataaac gcctgaacca
     9661 tcacatttgc cagatgttca ctgttacgca tctttgagtt cctccagtgc ctgttcaatt
     9721 tcatcgcctt tcggcacccg gtgatgccat tccgcacgcc cttgtataaa ggaaatgcct
     9781 gcccctttag tggtgtgggc aatcaccaca ttcggtttgc cttcctgttt aagctcttca
     9841 agcgtcgcga caaccgaagc catatcattg ccctgacatt cagtaacctg cataccgaat
     9901 gccagccatt tctctgccag aggatcggtg ttcatgattt cgcgcgttgg cccggctaat
     9961 tgcagattat ttttgtcatt aataatgacc aggttatcaa gcccgtaatg cgccgccgcc
    10021 agtgcagctt cccagttact gccctcagcc agctcaccat cgccagtaat taagaagata
    10081 cgacgacggc tttgactttt tttcgccgcc agcgcaagtc ctacagccac agggaaacca
    10141 tgccccagtg ctccggtatt cagttcaata cctggcgttt tttgccgaac cggatggccg
    10201 ggtaagtgtg aatttggctg ctgataagtg gaaagccatt ccagtgggaa gaatcctgct
    10261 tccgccagca cgcagtagta acatccaaca gcatggcctt ttgactgaat gtatatatcc
    10321 cggtcatcgt cgtgaaggcg atccggtgcg acattgagaa tacgaaaata gagtgcggtc
    10381 agtaattcaa cctgagagag atcggccccg gtgtgtccac ctgccgggct attggcattt
    10441 aagcgcacga tatggcggcg taccgcccgc gctttacgct caagggtctg gatatcatag
    10501 ctatagggat tcatttaata cctctgaatt tattttcaac atggaatatt tattccaccg
    10561 gggactaaaa aaaccagaaa tcccagggcg ttgcgcggca agacttacgc tgctgcatca
    10621 tactcaggaa cattctgcct ccttacaccg tcagttacat gtccgggtaa tcatcagttg
    10681 acctgatatc atcttgaata tatattcaat aatgattctt tattcaatta tgccgcctgt
    10741 tttttatttt tgtccatcgc tgattttaag gattggttaa agttagaact ccatcacact
    10801 taactgtctt cttttttcgc gtatgataaa agcctctaag ataagggcgg tacgttaatg
    10861 atttacgggg aaatatatgg ctaagcagga tgaacagcgg ctactggtga agatcgccac
    10921 tctttactat cttgaggggc gaaaacagtc tgacatcgcc caacttctct cgctttctca
    10981 gtcatttata tccagagcgc taacccgttg ccagaaagaa ggcgtggtta aaatcagcgt
    11041 tgtccagccg tcaaatatct ttctcaatct ggaaaaaggc attgaagagc gttacggcat
    11101 taaacaagcg attgttgtcg ataccgaaga tgatgccagt gatcacacca ttaaacgcgc
    11161 catcggctct gccgccgcgc actatctgga aacgcgttta aggccaaaag atttcattgg
    11221 cgtctcttcg tggagttcta cgattcgcgc gatggtcgat gaagttcacg cccaaaactt
    11281 aaaagccagc ggcgttattc agctgctggg cggcgtgggg ccaaacggca atgtgcaggc
    11341 aactattttg acacagaccc tggcccagca tctgaattgc gaagcctggt tgcttccttc
    11401 tcaaagcatt gaaggttcgg tagaagagaa aaaacgcctg gtggcaagcc aggatgtggc
    11461 tgacgttatc gccagatttg acgacgtcga tatcgccatt gtcggcatcg gtatccttga
    11521 accctcgcag ttgctaaaaa cctcgggcaa ctattatcac gaggatatgt tacaggtact
    11581 agcggatcgc ggtgctgtgg gcgatatctg cctgcactac tacgataaac atggtcagcc
    11641 cgtgttgcag gacgatgaag atccagtgat tggtatggca ctggataaaa tcaaaaaatg
    11701 cccgaatgtg gtggcactgg cgggcggcaa agacaaagtt gccgccatca aaggcgccat
    11761 gcagggtggt tatattgatg ttttgatcac tgatttcccc accgccagaa tgctgattgc
    11821 ggattaatcc tctcctccct ttcttctgcc cgcctttgcg aaaaaatctt ctcgcaaagg
    11881 tgattgctta aaaagccttt aatatcaata aactgaaagg attcaacaat cctgtttctc
    11941 gaggctttct caccctgtca ccacgcttct aagtgtgata gccagcacat ttgatgattt
    12001 tttctctagc tcaggaagaa taatctggct aaggtataaa taaataatca gtatcgaata
    12061 tatattcatg ccttaacaca aaatgtcaca gcagggcctg atgaagtaat aacgtgacca
    12121 tgaggaacgc ctcatctcct tccacgccag gatgcaatgt gactcaggag actacctatg
    12181 tttgcaggaa ataaagagtt cgttattgcg ttggatgagg gtaccaccaa tgcaaaagcc
    12241 atcgcacttg atggtttcgg taacgtggtg gcgaagtttt ctcaaccact ggctattcag
    12301 acgccacgtg aaggctgggt tgagcaatcc ggggaagcgt taatctctgc ctcgcttgat
    12361 gttattgccc aggccatcaa ccacgtgggg gcagaaaatg tagcggctct ggctatcagt
    12421 aatcagcgtg aaacggctat cggctggtat cgtcagagtg gaaaaccact caatgcggcg
    12481 attacctggc aatgcacgcg cagtgccgag ttttgtgaaa cactgcgtcg cgagggcaaa
    12541 gagcagcaaa ttaaaacgac tacgggtctt cctatcgccc cgctattttc cgcgtccaag
    12601 atgcgttggc tactggaatc tacacctgag ggtgtgcaac ttgccgacca gggtgaaatt
    12661 tgtctcggca ccattgatgc ctggttgcta tggaacttga ctgctggcga ttcattttgc
    12721 tgcgactact ctaacgccgc gcgaactcaa ttacttaacc tgaaaacagg cgagtgggat
    12781 gaggaaatgc tggcgttgtt tcacattcca cgcgctgcat tgccagaaat taaaccttcc
    12841 agtggcctgt tcggttatac ccgagggctt aaaacaattc cggatggtat tcccgtcatg
    12901 gcgatggtgg gcgattctca cgccgctctt tttggtcatg cgttgggaga agcgggatgt
    12961 gtgaaagcga cctatgggac cggttcttcc gtcatggccc ccgtcaactc tgctcagtgt
    13021 gatatcgcca cacttgccac aaccattgcc tggcatgatg gcgaaaatat tatttacggt
    13081 ctggaaggaa atatccccca caccggcgat gccgtggcat ggatggctga cagcaccggt
    13141 ttaagtgaac tgacagacgc ggagctggcc ctcgaactga ataccctgcc agcctcagtg
    13201 gattcgacta tgggtgttta ttttgttcct gctctcactg gcctgggcgc gccatggtgg
    13261 gatgacagcg ctcgcggcgt ggtgtgtggc ttaagccgtg gcgtaaagcg cgcgcatttg
    13321 attcgtgccg cactggaatc aatcacttat caaattgcag atgtagtgga ggcaatgcaa
    13381 cagcatgatg attttcactt atcggcgtta atggtggatg gcggcccgac caaaaatgac
    13441 tggttgatgc aatatcaggc ggatctgctg ggttgcccgg tgatgtgcag tgatgtgcca
    13501 gagctttcag ccattggtgc gggcctgctc gcccgcaaag cgcttatccc cggtaccgtt
    13561 gcagatttaa aagcgttatt aactgaacac agtgaattta aaccggacat ggcgcgtcat
    13621 cagcgactgc aaaagagatg gcaggagtgg cgtcatgcgg taaacagaac attatggaaa
    13681 ccggcatcgt caatttgacg aacaagacta accaacgtaa ggagaatacc ggcccataaa
    13741 taattcaggc tttacgttgt ttcaccgtac ccgacaataa caacacagag agaacattgt
    13801 catgaaatta cgtttaacac tgttaaccgc tgcgaccctg actgctcttt ctttttctgc
    13861 tcatgcggta gaaaaaggaa ccattatgat tatggtcaat tcactggata atccttatta
    13921 cgcctcggaa gcgaaaggtg ccagtgaaaa agcccagcaa ctgggatata aaacgaccat
    13981 tctttctcat ggtgaagatg tcaaaaaaca gaatgaactg atcgatactg caattggtaa
    14041 aaaagttcag ggcattattt tagataatgc cgactccact gccagtgttg cagccattga
    14101 aaaagcaaaa aaagcaggta ttcccgttat attaattaat cgcgaaatac ctgtggatga
    14161 tgttgcactg gtgcaaatta cccataataa cttccaggct ggctcggaag ttgccaacgc
    14221 atttgtcgag aaaatgggag agaaaggaaa atatgccgaa ctcacctgta atcttgctga
    14281 taataattgc gtaactcgct cgaaatcttt ccaccaggtt attgaccagt tccctgaaat
    14341 ggtcagcgtg gcgaagcaag atgccaaagg cacgttaatt gatggcaaac gaataatgga
    14401 cagtattctg caagcccatc ccgatgttaa aggggtgatt tgtggtaatg gtccggttgc
    14461 actgggagct atcgcagcac tgaaagccgc caatcgcaat gacgttattg ttgtcggcat
    14521 tgatggcagt aacgatgagc gcgatgccgt gaaagccgaa tcattgcagg ctaccgtgat
    14581 gttacaagca caagccattg ccgcgcaggg tgttaccgat ctggataatt acctgcaaaa
    14641 aggagttaaa ccagccaaac agcgggtgat gttccgcggc attctgataa ccaaagataa
    14701 cgcagataaa gtccaggatt tcaatatcaa atcctgatcc caggtcgttg cgccctcaca
    14761 atgagggcgc agaggagaat attatggata aaaaagtctg gctggcgctg gcaattctga
    14821 gcctgggtgg atgtcgcatc gtctcgcagc aggaacttgc cgatctcaaa tccccgccta
    14881 atcctcatat ggcaaatatc gatcagacct ggcagaaaaa catcgtgccg caggtggtag
    14941 aaaatgcgcg tccggttgcg gagttaatgg cggcgctaca ggcagaaaaa gatgtcgatg
    15001 ctgcctgtaa aacgctgggc tatcgttctc aggaagaaaa cccgtgtatt ttctacgtta
    15061 aggtcgaagg tagcatcacc aatattgatg cgaaatcccg tagcggaaaa atgaccatca
    15121 cagatatcag cggcacaaat attgtcgtgc agacggggcc aactcttcgc ggcacgcttc
    15181 tgcgagatgc atataaaggt gcaagctatg agcattttaa cgatcaggtt ttatttggtg
    15241 aatatggtcg tgctatcaat gagcaagcca tcaatatgat tcagtcggca aagcttaaaa
    15301 ccggggaaac ggttcaggtt tatggcgtat tttctgcatg ggatattccg caaactatcc
    15361 cggatataac gcctgcgaaa atagccaggg cgggaggcga gtaatatgtt gccgcaagaa
    15421 attgcccccc cccagactga tgaatcacct gtaatcatag aaacgcatga cttatcgcgt
    15481 atttatccgg gtgtggtggc gttagataac gtgaattacc gagtctatcg caataaagta
    15541 aatgtgctta ttggtgaaaa cggtgcgggt aaatccacaa tgatgaaaat gctggcgggg
    15601 gttgaaacac cttcctcagg caagatcatt cttgatggta aagaagtcac cctaaattct
    15661 acccatcagg ccgaaaaaca tggaattagt atcatttttc aggagctgaa tttattcccg
    15721 aatatgaatg tcatggataa tattttcatg gcgaatgaat tttttcagaa agggaagatt
    15781 aacgaaaaat atcaatatga attagccaaa tctctattag aaagactgga actgaatgtt
    15841 gatccctata cccaactggg agagctaggt attggccatc agcaactggt ggagattgcc
    15901 cgtgcccttt ctaaagatac ccgggtactg attatggatg aaccgacatc ggccctcagc
    15961 cagtcggaag taaaagtcct tttcaatgtg atcgcacaac tgaagcgccg tggtgtcacc
    16021 atcatttata tctcccatcg tctggaagag ttgatggaga ttggcgattt catcaccatt
    16081 ttccgtgatg gtcgctttat tagtgaacgc gccgtgagcg atgccagtat tccatggatc
    16141 atcgagcaaa tggtcggtga taagaaaaaa catttcgatt atcaaccagc tacacaaggt
    16201 gacactgtac tggcggtaca agggttaacg gcgctgcatc ccagcggtgg ttacaaactc
    16261 aatgatgtga cattcagcct gcataaaggc gaagttatcg gcatttacgg cttgctgggc
    16321 gcaggacgta ctgaactatt taagggattg gtgggcctga tgccctgtca gagtgggaag
    16381 atccagctta atggtgaaac catcgacaaa tgccatttcc aggcgcggct caaaaagggg
    16441 ctggcgttgg tgccagaaga tcgccagggc gaaggcgtcg ttcagatgat gtcgattcag
    16501 tccaatatga cgctatccga tttcagcctg caaggttttc gccgtgcctg gaaatggctg
    16561 aatccgcaaa aagagagcgc cagtgtgaaa gagatgatcc tgcaactggc gatcaaagtc
    16621 agcgatgccg aactccctat tacctcactc agcggcggta accagcaaaa agtggtactt
    16681 ggcaaagcgt tgatgaccca gccacaggtg gtgtttcttg atgaacccac acgcggcatt
    16741 gatgttggcg caaaaacaga tgtctatcag ctggtgggaa aaatggcaca acaggggctg
    16801 gcagtcatgt tctcttcttc agaactcgat gaagtcatgg cactggcaga tcgcattctg
    16861 gtgatggctg atggccgtat taccgctgat ctcccccggg gcgaagtcac ccgagagaaa
    16921 ctgattgcgg cttcaacacc tcaagattaa tcaggctgga atttcttatg aatcagaaat
    16981 atatgatcta tatgtacctg ttgaaggcca gaacgtttat cgctctgcta ttagtgatcg
    17041 ccttctttag cgtgatggtg cctaacttcc tgacagcctc taacctgttg atcatgactc
    17101 aacacgttgc gattaccggg ctgctggcaa tcggaatgac gctggtgatc ctgaccggcg
    17161 gcatcgatct ttccgtcggc gcggtagcgg ggatctgcgg aatggttgcc ggggctcttc
    17221 taacaaacgg cctgccattg tggagcggca atattatttt cttcaacgtg ccggaagtga
    17281 tcctctgcgt ggcggtattt ggcattttag tgggactggt gaatggcgcg gtaatcaccc
    17341 gctttggtgt cgcgcccttt atctgcaccc tcggtatgat gtacgtcgct cgtggctctg
    17401 ccctgctgtt taatgacggc agcacttatc ccaatctcaa cggcatggaa acgctgggca
    17461 acacgggttt cgccaccctt ggttctggca cgtttttagg catttatcta ccaatctggc
    17521 tgatgattgg ctttttgatt ttaggctact ggatcacgac taaaacgccg ctcggtcgtt
    17581 atatctatgc cattggtggc aatgaatctg ccgcgcgact ggcaggcgtc cccatcgtaa
    17641 aagttaaagt gttcgtctat gcgttctccg ggttatgcgc cgcatttgtc ggtttgattg
    17701 ttgcctcgca gctgcaaact gctcacccaa tgaccggcaa tatgttcgaa atggatgcta
    17761 ttggcgccac ggttcttggt ggaactgcac tggcgggtgg tcggggacgt gtctctggct
    17821 cgattatcgg tgcctttgtc attgtcttcc tcgccgatgg catggtgatg atgggtgtca
    17881 gtgacttctg gcaaatggtc attaaaggcc tcgttatcgt caccgccgtg gtggtcgatc
    17941 agttccagca aaaattgcaa aacaaagtca ttttgatgcg tcgccatgaa gaaaagatgg
    18001 ccgccgcacc tgccgtaaaa ccgctttcca gttaattaac atcattccac cgggcattaa
    18061 attgcccggc ttatacaggg gaaaaaatat gcaacgtaat tttcagggta agactgtagt
    18121 tattaccggg gcatgccggg gaattggggc cgggatcgct gaacgcttcg cccgcgatgg
    18181 cgcaaatctg gtgatggttt ctaacgctga acgcgtacat gaaactgcag agcaactgcg
    18241 ccaatgttat caggctgaca ttttgtcatt agaagtcgat gtgacagatg aagcgcaagt
    18301 acaggcactc tatgaacaag cggctgcacg ttttggcagc atcgatgttt ccattcagaa
    18361 tgcaggcatt atcaccatcg attattttga tcgaatgccg gctgctgatt tcgaaaaagt
    18421 gctggcggtg aacactacgg cagtctggct ctgctgccgg gaagcagcca aatatatggt
    18481 gaaacagaat cacggctgcc tgatcaacac ctcttctggc cagggacgtc aggggtttat
    18541 ctatacccca cactatgcag ccagcaaaat gggtgtgatc ggcattacgc aaagcctggt
    18601 gcacgagtta gcgcagtgga atatcactgt taacgccttc tgcccgggta ttatcgaaag
    18661 cgaaatgtgg gactacaacg accgcgtatg gggagaaatt ctcagtaccg atgacaagcg
    18721 ttatggcaaa ggcgagctta tggcggaatg ggtggaaggt attccgatga agcgtgccgg
    18781 taaacctgaa gatgttgccg gactggttgc cttcctcgcc tctgacgatg cgcgttatct
    18841 caccggtcaa accatcaaca tcgacggcgg cctgattatg tcgtaatgcc cctgcgggct
    18901 actaagagag atatcaaaat gatcgcaaaa gattttgctc aacaattgtt tgatctccac
    18961 gatcgcgtcg cctttgtcac tggcgcaggt agcggtatcg gacaaatgat cgctctggct
    19021 ttagccagcg caggcgcacg agtggtgtgt tttgatctac gggaagatgg cggactcaaa
    19081 gagacggtta atcatatcga agcacttggc ggacaagcct tgtattacac gggcgatgtt
    19141 cgggaattga gagatcttcg cgcagcagtc gctctggcaa aaacacattt tggtcgtctg
    19201 gatattgccg taaacgccgc tggaatcgct aacgcaaacc cggcgctgga gatggaaagc
    19261 gagcaatggc aacgcgttat cgatatcaac ctgactggtg tgtggaattc ctgtaaggcg
    19321 gaagctgagt taatggtaga gaacggagac ggttcgatta taaatattgc gtcaatgtcc
    19381 gggattattg tcaatcgtgg tctggaacag gcccactaca acagttccaa agccggcgtg
    19441 attcatttat ctaaaagtct tgcgatggaa tgggtgagta aggggatccg cgtgaactca
    19501 atcagcccag gatataccgc gacaccaatg aatacacgac ctgaaatggt acatcagact
    19561 cgggagtttg aaagccagac gccgattcaa cggatggcga aagtggaaga gatggcgggt
    19621 ccagcgctgt tccttgccag cgatgcggcg tcgttctgca cgggtgtgga tctggtggtc
    19681 gatggtggct ttgtctgctg gtaaaaatgg tttcgcccgc agccatcact gaccgcgggc
    19741 gaaagtgtaa agcaggtgcc ttaccaccct gacctgacaa ccggatatgc ggggattgct
    19801 ccccgcacaa cggctacttc ttcggttcgt aagcgagaac agccttaaac tcaatattac
    19861 gttcctttac ggtaaactca cacagcgagt ctccgaccag aagcgtaaat cccagcaccg
    19921 tcaaacacag cactatgact gccacaaggg catatttcgt cagcatgttt atattgcctc
    19981 catgcgaaga agggactacc atccaaattg tctggtttgg agtggcagcc ccagggttga
    20041 tagaaataac tcctggggct tcatctttcc agacctcaaa gttaacctga aaccggaaag
    20101 cttcaggcac ccgccgttat cttacccttc ctcaacaaaa tagctgttta tttatacagt
    20161 aagtcctgtt tcggacttct gttggcaaaa ctgatcattg cccttaaaac gacatcgccc
    20221 gcagccatca ctgaccgcgg gcgaaagtgt aaagcaggtg ccttaccacc ctgacctgac
    20281 aaccggatat gcggggatta ctccccgcac aacggctact tcttcggttc gtaagcgaga
    20341 acagccttaa actcaatatt acgttccttc accgtaaact cacacagcga gtctccgacc
    20401 agaagcgtaa atcccagcac cgtcaaacac agcactatga ctgccgcaag ggcatatttc
    20461 gtcagcatgt ttatattgcc tccatgcgaa gaagggacta ccatccaaat tgttcaggtt
    20521 tgggtaggta gccccagggt tgatagaaat atcgtcctgg ggcttcatct ttccagacct
    20581 cagagtaaac ctgaaaccgg aaagcttcag gcacccgcag ttatcttacc cttcctcaac
    20641 aaaatagctg tttatttata cagtaaatcc tgtttcggac ttctcactcc ccatttaatc
    20701 aactgaagct gaacccgttt ttttctgcat gcctcaatcg tcaaaattct tcgccgccag
    20761 ccagctctgc cataacttgt cacattgcaa aatgtagcca atttgcttca gtcaccatca
    20821 ccaaaaaaag aaacattcca gaacttccgc atctcgccca gccgctccca cttctatact
    20881 gaatagaaaa cgccaacata agagaaacct atgccattac ccgattttca tgtttctgaa
    20941 ccttttaccc tcggtattga actggaaatg caggtggtta atccgccggg ctatgactta
    21001 agccaggact catcaatgct gattgacacg gttaaaaatc agatcacggc cggagaggta
    21061 aagcacgata tcaccgaaag tatgctggag ctggcgacgg atgtttgccg tgatatcaac
    21121 caggctgccg ggcaattttc agcgatgcag aaagtcgtat tgcaggcagc ggcagaccat
    21181 catctggaaa tttgcggcgg tggcacgcat ccgtttcaga aatggcagcg tcaggaggta
    21241 tgcgataacg aacgctatca acgaacgctg gaaaactttg gctatctcat ccagcaggcg
    21301 accgtttttg gtcagcatgt ccatgttggc tgcgccagtg gcgatgacgc catttatttg
    21361 ctgcacggct tgtcacggtt tgtgccgcac tttatctccc tttccgccgc gtcgccatat
    21421 atgcaaggaa cggatacgcg ttttgcctct tcacgaccga atattttttc cgcctttccc
    21481 gataatggcc cgatgcagtg ggtcagtaac tggcaacaat ttgaagccct gtttcgctgc
    21541 ctgagttaca ccacgatgat cgacagcatt aaagatctgc actgggatat tcgccccagc
    21601 ccccattttg gtacggtgga agttcgggtg atggataccc cgttaaccct tagccacgcg
    21661 gtaaatatgg cgggattaat tcaggctacc gcccactggt tactgacgga acgcccgttt
    21721 aaacatcagg agaaagatta cctgctgtat aaattcaacc gtttccaggc ctgtcgctat
    21781 gggctggaag gtgtcattac cgatccgcat actggcgatc gtcggtcact aacggaagcc
    21841 accttgcgtt tactggaaaa aatcgcccct tctgcacata aaattggcgc atcgagcgca
    21901 attgaagcac tgcaccgtca ggtcgtcagt ggtctgaatg aagcgcagct aatgcgtgat
    21961 ttcgtcgccg atggtggctc gctaattggg ctggtgaaaa agcattgtga gatctgggcc
    22021 ggagaataag tcgggaattg ccttacagaa gaatatccct gacaatggtg ttttaactta
    22081 catttaacaa tgttatgaaa caccctttag aaaccttgac caccgcagca ggcattttgc
    22141 tgatggcttt cctctcttgc ctgctgctgc ccgcccccgc actggggctg acgctggcac
    22201 aaaaactggt gaccacgttc catctgatgg atcttagcca gctttacacg ttattgtttt
    22261 gtctgtggtt tttagtgctg ggcgctattg agtattttgt tctacgcttt atctggcgac
    22321 gctggttctc gctggcggat taaacgtcga ttatcgccga acttcgcggc atactttgct
    22381 tattctcctt cgcctaaagg aacatttatg gataagcaat cactgcacga aacggcgaaa
    22441 cgcctggctc ttgagttacc ctttgtcgag ctttgctggc cttttggccc ggagttcgat
    22501 gtttttaaaa ttggcggcaa gatttttatg ctgtcgtcgg agctgcgcgg cgtccccttt
    22561 attaatctga agtccgatcc acaaaaatcc ctgctaaatc agcaaatata cccaagcatc
    22621 aagccagggt atcacatgaa taaaaagcac tggatttcgg tgtatcccgg cgaggaaatc
    22681 tccgaagcgt tacttcgcga tctgatcaac gattcgtgga atctggtggt tgatggtctg
    22741 gctaaacgcg atcaaaaacg actgcgtcct gactcaaccg taaatcaata gcgcattttt
    22801 ctcgcttacc atttcatctc gatccttgta atctgctggc acgcaaaatt actttcacat
    22861 ggagtcttta tggatatcat ttctgtcgcc ttaaagcgtc attccactaa ggcatttgat
    22921 gccagcaaaa aacttacccc ggaacaggcc gagcagatca aaactctact gcaatacagc
    22981 ccatccagca ccaactccca gccgtggcat tttattgttg ccagcacgga agaaggtaaa
    23041 gcgcgtgttg ccaaatccgc tgccgataat tacgtgttca acgagcgtaa aatgcttgat
    23101 gcctcgcacg tcgtggtgtt ctgtgcaaaa accgcgatgg acgatgcctg gctgaagctg
    23161 gttgttgacc aggaagatgc cgatggccgc tttgccacgc cggaagcgaa agccgcgaac
    23221 gataaaggtc gcaagttctt cgccgatatg caccgtaaag atctgcatga tgatgcagag
    23281 tggatggcaa aacaggttta tctcaacgtc ggtaactttc tgctgggcgt ggcggcgctg
    23341 ggtctggacg cggtgcccat cgaaggtttt gacgccgcca tcctcgatga agaatttggt
    23401 ctgaaagaga aaggctacac cagtctggtg gttgttccgg tgggtcatca cagcgttgaa
    23461 gattttaacg ctacgctgcc gaaatctcgt ctgccgcaaa acattacctt aaccgaagtg
    23521 taattctccc ttgccgggca tctgcccggc tatttcccct cagattctcc tgatttgcat
    23581 aaccctgttt cagccgtcat cataggctgc tgttgtataa aggagacgtt atgcaggatt
    23641 taatatccca ggttgaagat ttagcgggta ttgagattga tcacaccacc tcgatggtga
    23701 tgattttcgg tattattttt ctgaccgccg tcgtggtgca tatttttttg cactgggtgg
    23761 tactgcggac cttcgaaaaa cgtgccatcg ccagttcacg gctttggttg caaatcatta
    23821 cccagaataa actcttccac cgtttagctt ttaccctgca ggggattatc gtcaatattc
    23881 aggcggtatt ctggctacaa aaaggcaccg aagcggcaga tattctgact acctgcgcgc
    23941 agttgtggat catgatgtat gcgctgcttt cagtcttctc gttgctggat gtgattttga
    24001 atctggcgca gaaattcccg gcggcatctc agttaccgct gaaagggatt tttcagggga
    24061 ttaaactgat cggcgcgatt ctggtcggca ttttgatgat ctcgctgctg attggtcagt
    24121 cgccagcgat tctgatcagt ggtcttggtg caatggctgc cgtgctaatg ttggtattta
    24181 aagatccgat tcttggtctg gtggcaggta ttcagctttc cgcgaacgat atgctgaaac
    24241 tgggtgactg gctggagatg ccgaaatacg gcgcggatgg cgcggtgatc gatattgggt
    24301 taaccaccgt caaagtgcgt aactgggaca ataccattac cactattccc acctggtctc
    24361 tggtttctga ctcctttaaa aactggagcg ggatgtcagc ttctggcggg cgacgtatta
    24421 aacgcagtat cagtattgat gtcaccagta ttcgttttct tgatgaagac gaaatgcaac
    24481 gcctgaataa agcgcatttg ttaaagcctt atttaaccag ccgccatcag gaaattaatg
    24541 agtggaatcg ccagcaaggt tctacggagt cggtattaaa cctgcgccga atgaccaata
    24601 ttggaacctt tcgtgcctat ctgaacgaat atctgcggaa tcatccgcgg attcgcaaag
    24661 atatgacctt aatggtacgc caactggctc ctggtgataa cggtttaccg cttgagatct
    24721 atgcgtttac caacaccgtg gtgtggctgg aatatgaaag cattcaggct gatatattcg
    24781 atcacatatt tgcgattgtc gaagagtttg gtctgcgact tcatcagtcg ccaaccggca
    24841 atgatattcg ctctctggcg ggtgcattta agcagtaatt aaaaaaaccg ctctcatcga
    24901 atggatgaga gcggctttca gattgttgac atcattttgt cggatgcggc gtgaacgcct
    24961 tatccgacct acagaatcgt tacaaattgt aggcccgata agcttgcgca tcgggcaaat
    25021 cagcctcaca tcagcttaat tcactttcct acgcaatgtt ttaaacgcca caaacaggaa
    25081 cacaatccac accggcagca gaatcgctga caagcgcata tcatccatcg tgcacatcag
    25141 cagcaaaatc atgccgagga aggcgatgca aagatagttg ccgaacggat agagcagcgc
    25201 cttaaattgt gtttcacgcc cctgacgtcg catcgccgcg cgaaaacgca gatgcgccag
    25261 gcagatcata atccagttca acagcagcgt tgctaccacc agcgccatca gcagaccaaa
    25321 cgctttttgc ggcagcagat agttgattaa caccaccagc gaagtgatcg ctccggaaag
    25381 catcagcgag ttaatcggca cgccgcgacg gctgacgcga gtcaaaaact ttggcgcatt
    25441 accctgcaca gaaaggccaa acagcatgcg gctgttagag taaaccccgc tgttatacac
    25501 cgacagcgat gccaccagaa tgacgaagtt cagcgcagaa gctaccacgt tgctgtcgag
    25561 attatggaaa atcatcacaa acgggctact attggatttc acttccaccc acgggtagag
    25621 cgccagtaaa accaccagtg aaccgatata aaacagcagg atgcgataca ccacctgatt
    25681 cactgcttta gggatgcttt tttccggatc gcgcgcttca gccgcagtaa tcccaatcag
    25741 ctccagaccg ccgaaggaga acataattac cgccagcgac aaaatcagtc cattccagcc
    25801 ggtggcaaag aaaccgccgt agcgccagag gttatcgata ctggctttct caccgccgtg
    25861 cccagaaaac agcagccaca ggccaaagcc gatcataccg atgatcgcca gcactttaat
    25921 cagcgcaaac cagaactcgg tttcgccata taagcgcacg ttcaccaggt taacggcgtt
    25981 gatgataata aagaaggcgg cagcccaaat ccacgttgga acatccggga accagtactg
    26041 catatagatg cccgcagcgg tcagctctgc cattcccacc agcacgaaca ttacccagta
    26101 gttccagcca gagaggaagc ccgcaaacgg tccccagtat ttataggcaa agtgggcaaa
    26161 tgaaccggat accggctctt cgaccaccat ctcgccgagc tggcgcataa tcaggaaagc
    26221 gatgatcccg gcgacgccgt agcccagcaa tacagccgga cccgccatct gaatcgccgg
    26281 gccaatgcca agaaacagac cagtaccaat tgcgccaccc agcgcaatca gttgaatatg
    26341 acggttatgt aatccgcgat gaagcgtcgg ctcttgattc gacgcagtat cttccgatac
    26401 ggttgacgcg tttttcacgc ctttcccctg tgtgtctttt ttgttgaggg gcacctttta
    26461 acatttagtg cccatcgtcg caagacacaa tccacacggt taaacggggt atcctgcttt
    26521 tatttccgta cccgatgtcg gtgcagccac atcagcttat acgccgccgg gataataaac
    26581 agcgacagca aaggcgcggt gatcatgccg ccaatcatcg gcgcggcaat ccggctcatc
    26641 acctctgacc ctgctcctgt tccccacaga atcggcagca gaccagcgat aatcaccgcc
    26701 accgtcatcg cttttgggcg cacgcgcagt accgcgccgt gatataacgc ctcatccagc
    26761 ttctgctcgc tgaatgtttg cggattattc aacgacggct cggcctctat ggcgtgacgt
    26821 aaatacatca gcatcaccac gccaaattcg gcggcgaccc cggcaagggc aataaaaccg
    26881 gtgcccgtcg ccacggaaag atgaaagccc atccaccaca gcaaccagat gccgcccacc
    26941 agcgcaaacg gtacgctgct gataatcagc aacgcttcgc ccacccgacg gaacgccaga
    27001 tacaacagca cgaagatgat catcagcgtc atcggcacca tcagtttcag cttatggttg
    27061 gcgcgctcca gtagctcgaa ctgcccggag aatgccacgc tggtacccgg ttttaactgc
    27121 actttttcag ctatcgcttt ttgcagatcg tgaaccaccg acaccatgtc acgatcgcgg
    27181 gcatcgatat aaatccagct cgtcgggcgc gcattctcgg ttttcagcat cgacggtccg
    27241 gtagagactt taacgtcggc cacgtccgcc agagtgattt gctgcttcat cggcgtgagg
    27301 atcggcaact ggcgcagtgc ctgcgggcta tcgcgccagc tttgcggata acgcagatta
    27361 attggataac gagcaatccc ttccaccgtt tcgccaacca tcgccccgcc caccgcagaa
    27421 gtcacaaaca actgcacatc cgccaccgtc ataccgtaac gcgcggcttt ttcacggtta
    27481 atctcaacgt tgatataacg cccaccttcc agacgctcag caagcgcaga agccacgcct
    27541 ggcaccgttc gcgccacttc ttcaatttgc tccgccatcg tgtcgatatc cgccagcaca
    27601 gtgccggaaa ctttaatgcc gatagggctt ttaatgccag tcgagagcat atcgatacgg
    27661 ttacgaattg gcggcaccca cagattcgcc agccccggca gacgcacggt gttatccagt
    27721 tcctcaatga ttttgtccat cgtcatgcca ggccgccact gatcctgcgg cttaagctgg
    27781 atagtcgttt ctaccatctc cagtggagct gaatcggtgg cggtttccgc tttcccggtt
    27841 ttgccaaata cccgcgccac ttccggtacg ctcataatca gcttgtcgtt tttttgcagc
    27901 atgctcgccg cctctgctgc ggaaatcccc ggcagcgtcg atggcatata caacaagtcg
    27961 ccttcattga tctgcggtaa aaattccccg ccaactttat tgagcggcca gagaaccgtc
    28021 agcaccgaaa gcgctgccac caacagcgtg gttttcggcc agtgcagtac tttcagcaac
    28081 agcggatgat aaacacgaat caaaaagcga ttgagcgggt tactgctttc cggcggaatt
    28141 ttgccacgga tccagtagcc catcaggatc gggatcacta cgatcgccag tagcgccgcg
    28201 cccgccatcg cataggtttt ggtgaacgcc agcgggccaa acagacgacc ttcctgccct
    28261 tccagggtga agatcgggat aaacgacagc gtgataatca gcagactgat aaacagcgcc
    28321 ggccccactt caacagatgc atcggtgatc acctgccagc gcgttttatt atccagcgtg
    28381 gcgtcaggat gctggtgctg ccactcttcc agccgtttat gcgcattctc gatcatgacg
    28441 atagcggcat cgaccatcgc cccgacggca atcgcaatgc cacccaacga cataatattg
    28501 gcgttcagtc cctgaaagtg catgacaata aaagcaatac acaaccccag cggcaacgaa
    28561 ataatcgcca ccagcgccga gcgcacatgc cagagaaaca gcgcacagac taccgccacc
    28621 acaataaact cttccagcaa cttgccgctg aggttgtcaa tggcgcggtc aatgagctgg
    28681 ctgcgatcgt atgttgtaac tatctccacg ccttccggca gactgctttt cagcgtttcc
    28741 agtttgtcct tcacagcggc gatcacttca cgggcgtttt tgccggaacg caggatcacc
    28801 accccgcccg ccacttcgcc ttcgccgttc agttcggcaa tgccccggcg catctccggg
    28861 ccaacctgga ccttcgcaac atcacgcaga taaacgggca cgccattttc actggctttt
    28921 aaaacgatgt gattaaagtc gtcgagcgtt tgcagatagc cgctggcgcg caccatatat
    28981 tccgcttccg ccagttctat cgacgaaccg cccgcttcct ggtttgaagc atccagcgcg
    29041 ctttttacgt cggcgagact gatgccatac tgcgccaggc gctgaggatc gctaaccacc
    29101 tgatactctt tcaccacacc gcccaccgac gccacttccg caacgtcagg gatggttttt
    29161 agctcatatt tgagaaacca gtcctgcaat gagcgtaaat cggccagatc gtgcttaccg
    29221 ctgcgatcca ccagtgcata ttcatagatc cagccaacac ccgtggcatc tggccccagc
    29281 tcggcgctga ctcccgcagg cagcttaccc tgtacctggt tgaggtactc cagcacccgc
    29341 gagcgcgccc agtacggatc ggtgccatct tcgaaaatga catacacata agagtcgcca
    29401 aactgcgaga aaccgcgcac agtcttcgcg ccaggcaccg acaacatggt ggtggttagc
    29461 ggataagtca cctgattttc aacgatttgc ggtgcctgac cgggatagct ggttttaata
    29521 atcacctgca catcggaaag atccggcagt gcatccactg gcgtgttaat gatggtccag
    29581 gtgccccaga tactcagaaa caacgcgccc atcagcacca gaaaacggtt cgccaccgag
    29641 cgacgaataa tccattcaat cattggctat tccctcaatg cgcatgggta gcactttcag
    29701 agcgcatccg ctccagtgcg ccagaaatat tggcttcaga atcaatcagg aacaggccgc
    29761 tggaaaccac cttttcacct tccgccagac cagagcgtaa cgcggtgacg ccttgcgatg
    29821 cctggaaaac ggcaacgcgt ttcggtacaa agcgcccgtc agcatcaacg gtaatcaccc
    29881 gctgttcgtt gccggtatca atcagcgcct gtgacggaat gagcagcatc ggttcgctgg
    29941 cggtattgag ttgcaacccg gcgttcatgc ccggttttag cgcctcgtcg gcgttgtcga
    30001 tttccagacg caactgcagc gtgcgggtcg cggcatccac gccgggtagc agggtccatt
    30061 tgcgaatggt gagtgtttta tcaggtcgcg ccggaacggt gagcgtaaac tgcgaggcat
    30121 ctttcaccag ccaggcgatg gactccggga tcgcagcagt gacccacacc gggtccatac
    30181 cctgaatttt cgcgaccacg ttatctttgg cgatattcat tcccgcgcgc agatcaaacg
    30241 cggtgatcac gccatcaatg ggcgctttga gcgtaaagcg agtctggatt ttttgcgtgg
    30301 cgatcaggcg gcgaatatca gcctccggca ttcccgccag tcgcagtcgc tcaagaatgc
    30361 cttcagtctg ggtcgccgta ccgccggttt cgcgcagcag taaatactca ctctgcgctt
    30421 ccacccagtc gggaatggtc aggtcgagaa gcggcgcgcc cttttgcact ttatcgccca
    30481 cggtaagcgg atacaccttg tcgataaacc cggcggcgcg ggcctgcaca atggcatact
    30541 gatactcgtt gtaactgaca ttcgccggga aactctggga aaaagtcagc ggcccgcgcg
    30601 tgacggtcgc cgttttcacc cccagattct gcgtctgagt cgggtcaatg cgcacaccag
    30661 acgcagaact ctcttcatcg gcatatttcg gcaccagatc catatccata aacggcgatt
    30721 tccctggttt atcgaaccgc gtattgggat acatcgggtc gtaccagaat aagactttac
    30781 gttctgcggt cgacgttttt tctgcgggcg gttcctcctt tgcaaaccag gtaaaacctg
    30841 ccgcagaaat aataccgccc gcgatcatgc tgccgataat aagcgcgatt tttttcatct
    30901 cattaaaccc ggattactgg ctgactttaa tatcctgtaa taaagaaaga ttgccctgct
    30961 ggacaaaatt aaacgctact ttatcgccgg ttttaatttc gctcattttc gtctgcgggg
    31021 tgatggtaaa gcgcatagtc atctccggcc agttcacggc agcaatcgga tcgtgatgga
    31081 tggtgatttt tttgctttcc agatcaacac cttttaccac gccagtggcg ctaataacct
    31141 gtggttgtgc ttcgctcatg gtttcatgat gatgttcgtt agcctgggca ttaaagccaa
    31201 taacggtaaa cagactgaac attgcgactt gcagtgcttt tttcatttat tctctcctgg
    31261 agttaaaaat tattgctgcc aaccgccccc taaggcggta tataaagaaa tttcgttaac
    31321 ctgacgggca taattcaggt caagtaaagt ttgtcgggtt gcaaataaag aacgttcagc
    31381 atccagcact tccagataac tgactgcgcc gtgctgatat aatgcccgcg cccgttgcag
    31441 ggtaatttgc agcgacgcaa gataacgctg ctgggcgctg atttgatcgt tcaggctttg
    31501 acgtaatgcc agcgcatctg ccacttcttt aaaggcgttc tggattttct gttcataatt
    31561 caccaccgac tgctgctggc gaatttcggt gatatccaga ttggcctggt tgcgtccggc
    31621 attaaaaatg gggatctcaa ttttaggaat aaaattccac atcccgctgc tggcgttaaa
    31681 taatgacgac agatcgctgc tggcggttga tattccgctg gtcaggctga tagacgggaa
    31741 aaatgccgca cgcgcagcac caatattggc attagccgcc attaacgcgt gttcagcttc
    31801 cataatatca gggcgctgca ataagatttg cgacgacaag cccgccggta atttaacgct
    31861 ttgcaggctg tcgctgttta ctgtctgcgc ttgcggcagc ttgccgtagc ttcccaataa
    31921 cagttgcaat gcattattcg cctgcgccag ttccccctga cgtttagcga tgtcgctgcg
    31981 ggtactttct atcaccccgc gagcctgttc cagcgccaga acattgctgc taccggtcaa
    32041 cagttgtttt tcgacaaacg catatgactg ctgataatta cgcagcgttt cttcggctat
    32101 ttgcaattgc gcatacgcca gttgctgatt gaaatagctt tgcgcgacat tagaaaccaa
    32161 cagaatatgc accgcgcgct gagcttcctc agtggctaaa taattttggc gctcggcttc
    32221 gctcatgttc tttaagcgac cgaaaaaatc gagatcaaag ctggcgttaa ggccagtcga
    32281 taactcccgc gtcgtggctg aatcgccttt aagattgccg ctccagctgc cgctgccctc
    32341 gccattgagc tgtgggtagc ggtcggcatc ggtcagacga tattgcgccc gcgcttcctg
    32401 cactttcagc gccgccatgc gcaaatcccg gttattcacc aacgcctcgc taatcagcgt
    32461 cttcacctga ttatcaacaa aaaaggtgcg ccagcccgcg ttctggtagt tatctgctgc
    32521 gttaaccagg ccgttctggc tgagtgagaa ctgctgcggc acgggcattg ccggacgctg
    32581 ataatccggt gccagtgaac aaccggttag cgcaagggcc acacaaaatg gcagaagttt
    32641 acaaggagac ataggctcat aatttctggt gattttatgc tgccaacttt actcgccagg
    32701 ctctgatttt ccggtgacag gaaaatgaca aaattgtcat tttgccaata agcgattgcc
    32761 atctgatccc gctactctag aattgcccgg gcaacatgcg gaggaaatat gaaactgttg
    32821 attgtcgaag atgaaaagaa aaccggagaa tacttgacca aagggttaac cgaagccggt
    32881 tttgtggtcg atttggccga caacgggctg aatggctacc atctggcgat gaccggtgat
    32941 tatgatctga taatcctcga tattatgctg ccggacgtga acggctggga tatcgtgcgc
    33001 atgttgcgtt ccgccaataa ggggatgccg attctgttgc ttaccgcgct tggcaccatt
    33061 gaacatcgcg tcaaggggct ggagttgggg gcggatgact atctggtgaa gccgttcgct
    33121 tttgctgaac tgctggcgcg ggtgcgcact ctactgcggc gcggagcggc ggtaattatc
    33181 gaaagtcagt ttcaggttgc cgatctgatg gtcgatctcg tcagccgcaa agtcacccgc
    33241 agcggcacgc gcatcactct gaccagtaaa gaatttactc tgctggagtt tttcctccgc
    33301 catcagggcg aagtgctgcc ccgctcgctt atcgcctcgc aggtatggga catgaatttt
    33361 gacagcgata ccaacgccat tgatgtggcg gtgaagcggc tgcgcggcaa aatcgacaac
    33421 gactttgagc caaagttgat tcagaccgtg cgcggcgtgg gttacatgct agaggtgccg
    33481 gatggtcagt aagccatttc agcgcccgtt ttcgctggca acccgcctga ccttttttat
    33541 cagcctcgcc accatcgcgg cgtttttcgc ctttgcatgg atcatgatcc actcggtaaa
    33601 agtgcatttt gccgagcagg atattaatga tttaaaggag attagcgcca cccttgaacg
    33661 ggtacttaat caccctgacg aaacgcaagc ccgacgctta atgacgctgg aagatatcgt
    33721 cagtggttat tccaatgtgt tgatctccct ggcaaatagc cacggtaaaa cggtgtatca
    33781 ctcccccggt gcgccggata tccgcgagtt tacgcgtgac gccatacccg ataaagacgc
    33841 gcaggctggc gaggtgtatc tcctttccgg cccgacgatg atgatgccag ggcacggtca
    33901 cgggcatatg gaacacagca actggcggat gattaacttg ccggttggcc cgctggttga
    33961 cggcaaaccg atttatacgc tctacatcgc gctttcgatc gattttcatc ttcattacat
    34021 caatgatttg atgaataaac ttattatgac cgcatcggta atcagcatcc tgatcgtctt
    34081 tatcgtgctg ttggcggtac ataaaggtca cgcgccgatc cgcagcgtca gccgtcaaat
    34141 ccagaatatc acctcgaaag atctcgacgt tcgccttgac ccgcagaccg tgcccattga
    34201 gctggaacag ctggtactgt cgttcaacca tatgatcgag cgtattgagg atgtctttac
    34261 ccgacagtcc aatttctcag cggatatcgc ccacgaaatt cgcacaccta ttactaatct
    34321 cattacgcaa acggaaatcg ccctcagcca gtcgcgcagc cagaaggagc tggaagatgt
    34381 gctctactct aatctcgaag aactgacgcg aatggcgaaa atggtcagcg atatgctgtt
    34441 tctcgctcag gccgataaca accagctaat ccccgaaaag aaaatgctca acctggcgga
    34501 tgaagtcggc aaagtgttcg attttttcga ggcgttagca gaagatcgcg gcgtggagct
    34561 gcggtttgtt ggcgacgagt gtcaggttgc gggcgatccg ctgatgctgc gtcgggcgtt
    34621 aagcaacctg ctttctaatg ccctgcgtta tacgccaacc ggagagacaa ttgttgtgcg
    34681 ctgccagacg gtcgatcacc tggtgcaagt taccgtcgaa aaccccggta cgcccattgc
    34741 gcccgaacac ttaccgcgat tgtttgaccg tttctaccgc gttgatcctt cccgccagcg
    34801 aaaaggcgaa ggtagcggca ttggcctggc gatagtgaaa tcgattgttg tcgcgcataa
    34861 aggcacagtt gcggtaacgt cagatgctcg ggggacaagg tttgttatca tattacccgc
    34921 ttaatccttc agcaaacggc aacttttata accagtgtaa aaataacatg ccgcaatagt
    34981 aatacctatt aatgataaaa agctgtcaca attcataaaa aaccttaata tacgccaccc
    35041 taaacataac cagcgttaat gtaaggtttt tgtgtggact ggcttcttga tgtttttgct
    35101 acctggctct acggcttaaa agtaatcgcg ataacgttag cggtcattat gttcatcagc
    35161 gggctggacg atttttttat tgatgtcgtc tactgggtac gccgcattaa acgcaagttg
    35221 agtgtttatc gccgctaccc gcgaatgagt taccgcgaac tgtataaacc agatgaaaaa
    35281 ccgttagcga ttatggttcc ggcgtggaat gaaacgggcg tcatcggcaa tatggccgag
    35341 ctggcggcga ccacgctcga ctacgaaaac tatcatatct ttgttggcac ctaccccaac
    35401 gaccccgata ctcagcgtga tgttgacgaa gtgtgcgctc gcttcccgaa cgtgcataag
    35461 gtagtctgcg cgcgtcctgg ccccaccagc aaagccgact gtctgaacaa cgtgctggac
    35521 gccatcaccc aatttgaacg tagcgccaat ttcgcttttg ctggttttat tctgcatgac
    35581 gccgaagatg tgatttcacc aatggaattg cgtctgttca actatctggt cgagcgtaaa
    35641 gatctgattc agatcccagt gtatccgttc gaacgcgaat ggacgcactt caccagcatg
    35701 acctacattg atgagttttc agaactgcat ggcaaagatg ttccggtgcg tgaagccctc
    35761 gccggacagg tgcccagcgc aggagtcggc acctgtttca gccgccgcgc cgtgaccgca
    35821 ctgttagctg acggtgacgg tattgctttc gacgtgcaga gtctgactga agattacgac
    35881 attggcttcc gcctgaaaga gaaaggtatg acggaaattt ttgtccgttt tccggtggtg
    35941 gacgaagcca aagaacgcga gcagcgtaaa tttttacagc atgcgcggac gtcaaacatg
    36001 atctgtgtgc gcgaatattt ccccgatacc ttttcgactg cggttcggca aaaatctcgc
    36061 tggatcatcg gcattgtttt ccagggcttt aaaacccaca aatggacctc cagcctgacg
    36121 ctgaactact ttctctggcg cgaccgcaaa ggggcaatca gtaactttgt cagcttcctc
    36181 gcgatgctgg tgatgatcca gcttttgctg ttgctggcgt atgaaagttt gtggcccgat
    36241 gcctggcatt tcctttctat tttcagcggc agcgcatggt taatgactct gctgtggcta
    36301 aactttggct tgatgattaa ccgcatcgtg cagcgggtga ttttcgttac tggctactac
    36361 ggcctgacgc aggggctgct atctgtcctg cgtcttttct ggggcaacct gattaacttc
    36421 atggccaact ggcgcgcgtt aaaacaggta cttcaacacg gcgatccacg tcgtgtcgcg
    36481 tgggataaaa caacgcatga cttccccagc gtgactggcg atacccgctc gttgcgtccg
    36541 ttaggtcaaa tcctgctgga aaatcaggtc atcactgaag aacaactcga tacagcactg
    36601 cgtaatcgcg tcgaaggtct acgcctgggc ggttcaatgc tgatgcaggg gctgattagc
    36661 gccgagcaac tggcacaggc gctggcagag caaaacggcg tggcgtggga atccatcgat
    36721 gcctggcaga tcccttcctc gctgattgcc gaaatgccgg cctccgtggc gttgcattat
    36781 gcggtactgc cgctgcgtct ggacaatgat gagttaattg tcggcagtga agatggtatt
    36841 gacccggttt cgctggcggc cctgacgcgt aaagtcggac gcaaagtgcg ttacgtcatt
    36901 gttctgcgag gacaaattgt caccggatta cgccactggt atgcacgccg acgcggtcac
    36961 gatccgcggg caatgttgta caatgcagtt cagcatcagt ggctcacgga acagcaggcc
    37021 ggtgaaatct ggcggcaata tgtgccgcat cagttcctgt tcgcagaaat actgaccacg
    37081 ctcggtcata ttaatcgttc agcaattaac gtgttgttat tgcgccatga acgcagttct
    37141 ctgccgctcg gcaagttttt ggtcaccgaa ggcgttatta gccaggaaac gttggatcgc
    37201 gtcctgacaa ttcaacgcga attacaagtt tcgatgcaat cactattact caaagcaggt
    37261 ttaaacactg aacaggttgc acaactggag tccgaaaatg aaggagaata accttaatcg
    37321 cgtcatcgga tggtctggtt tactgctgac atctttattg agtaccagcg cactcgcaga
    37381 caatatcggc accagcgcag aagagctggg gctgagcgat tatcgccatt ttgttattta
    37441 tccccgtctc gataaggcgc tgaaggcaca gaaaaataac gacgaagcaa ccgccatccg
    37501 cgaatttgaa tatatacacc agcaggtgcc ggataatatt ccgctgactt tataccttgc
    37561 ggaagcctat cgccattttg gtcatgatga ccgggcgcgg ctgttgcttg aggatcaact
    37621 gaaacgtcac cctggagatg accggcttga gcgcagtctg gcggctattc cagttgaagt
    37681 gaaaagcgtt acgactgttg aagaactgct tgcccagcaa aaagcgtgcg atgctgcgcc
    37741 gaccctgcgt tgtcgcagtg aagtcgggca gaatgccctg cggctggcac aattacctgt
    37801 cgccagagcg caacttaacg atgcgacgtt tgctgcatcg ccggaaggaa aaacgttgcg
    37861 aaccgatctg ctgcaacggg caatctacct gaaacaatgg tcccaggcag atacgctata
    37921 taatgaagca cgccagcaga acacattaag cgcggcagaa cgccgtcagt ggtttgacgt
    37981 gcttcttgcc gcgcagctgg atgatcggat cctggcgctg caatcacagg ggatcttcac
    38041 cgatcctcag tcatatatta cttatgcgac cgcgctggct tatcgtggcg aaaaagcacg
    38101 cctccagcat tatctcattg aaaataagcc gctgtttacc acggacgcac aagagaaaag
    38161 ttggctctat ctgttatcta aatacagcgc caaccccgtt caggcgttgg cgaattatac
    38221 ggtgcagttt gccgataacc gccagtatgt tgttggcgcg acgctaccag tgctgttaaa
    38281 agaaggtcag tacgacgcag cgcaaaaact gctcgccacc ctccccgcca atgaaatgct
    38341 tgaggagcgt tatgccgtta gcgtagcaac ccgtaacaag gctgaagctc tgcgtctggc
    38401 acgattgctg tatcagcaag aaccggcaag tcttacccgt ctggatcaac tgacctggca
    38461 actgatgcag aacgggcagt cacgcgaagc tgccgattta ttgctgcaac gctatccttt
    38521 tcagggcgat gcgcgtgtca gccagacgtt aatggcgcga ctggcgtctc tgctggaaag
    38581 tcatccttac ctggcaacgc cggcgaaggt ggcaatttta tcgaaacctt taccgctggc
    38641 ggagcaacgt cagtggcaaa gtcagttgcc gggtattgct gataattgcc cggcaatagt
    38701 tcgcttgctg ggcgatatgt cgccttctta cgatgctgcc gcctggaacc gtctggcaaa
    38761 gtgttatcgg gacacgctac ccggtgtggc gttgtatgca tggcttcagg ccgaacaacg
    38821 acaaccggac gcctggcaac atcgtgcggt agcctatcag gcgtatcagg ttgaggacta
    38881 tgccaccgca ctgacagcct ggcagaaaat cagtcttcac gacatgagca atgaggatct
    38941 gcttgctgcc gccaataccg cccaggcggc aggaaatggc gcggctcgcg atcgctggtt
    39001 acaacaggca gaacaacgtg gactgggaaa caatgccctc tactggtggc tgcatgcgca
    39061 acgttacatt ccaggtcagc cggaactcgc actgaacgat ctcacgcgct caatcaatat
    39121 tgcgccttct gccaacgctt acgttgcgcg ggcaacaatt tatcgccaac gtcataatat
    39181 cccggcggcg gtgagtgatt tgcgcgccgc gctggaactg gaaccgaata atagcaacac
    39241 ccaggcagcg cttggttacg ccttgtggga tagcggtgat atcgcacagt cgcgggaaat
    39301 gctcgaacag gcgcataaag ggctaccgga cgatccggca cttatccgac aactggccta
    39361 cgtgaaccaa cgtctggatg acatgcctgc gacgcagcac tacgcccgac tggtgattga
    39421 tgacattgat aatcaggcgc tgataacccc actgacccca gagcaaaatc agcaacgctt
    39481 caatttccgc cgtctgcatg aggaggtcgg tcgccgctgg tcgttcagtt tcgattcttc
    39541 tatcggcttg cgttccggcg caatgagtac cgctaacaat aatgtcggcg gcgcagcgcc
    39601 agggaaaagc tatcgtagct acgggcaact ggaagccgag taccgcctcg gacgcaatat
    39661 gctgctggaa ggcgacctgc tctcagttta tagccgcgtc tttgccgata ccggtgaaaa
    39721 cggggtgatg atgccggtga aaaatccgat gtccggcacc ggtctgcgct ggaagccact
    39781 gcgcgatcag atctttttcc tcgccgtcga acagcagttg ccgctgaacg gacaaaatgg
    39841 cgcatccgat accatgctgc gcgccagcgc ctcattcttt aatggcggca aatacagcga
    39901 cgaatggcac ccgaacggtt caggctggtt tgcccaaaac ctgtacctcg atgcggcgca
    39961 atatatccgc caggatattc aggcgtggac ggcagattat cgcgtcagct ggcatcagaa
    40021 ggtggcgaac gggcagacta ttgagcctta tgctcacgtt caggacaacg gctatcgtga
    40081 taaaggcact cagggcgcgc agcttggcgg tgtcggggtc cgctggaata tctggaccgg
    40141 cgagacgcac tacgacgcct ggccgcacaa agtcagtctc ggcgtcgaat atcaacatac
    40201 ctttaaggcg attaatcaac gtaacggaga gcgcaacaac gcgtttctca ccattggagt
    40261 gcactggtaa atgcgtaagt tcattttcgt attgctgaca ctgcttttgg tcagcccttt
    40321 ttcctttgcg atgaaaggta ttatctggca accacaaaac cgtgatagtc aggttaccga
    40381 tacccagtgg caggggctga tgagtcagtt acgtttgcaa ggcttcgata cactggtttt
    40441 gcaatggacc cgttacggcg atgcattcac ccagccagaa cagcgcgcgt tattgtttaa
    40501 gcgtgctaca gctgcgcaac aggctggcct gaagctgatt gtcgggctga acgccgatcc
    40561 ggaatttttt atgcaccaga aacagtcgtc cgcagcgctg gaaagctatc ttaatcgcct
    40621 gctggctgcc gatctccagc aagccagatt atggagcgcc gtgcctggcg taacgccgga
    40681 tggctggtac atcagcgcgg aaattgacga cctgaactgg cgcagcgaag ccgcccgtca
    40741 gcctttgcta acatggttaa acaacgcgca gcggttgatt agcgatgttt cagcaaaacc
    40801 ggtttatatc agtatttttt tcgccggaaa catgtcgcca gatggctatc gccaactgct
    40861 ggaacaagtt aaagcaaccg gtgttaatgt ctgggtacag gatggcagcg gcgtggataa
    40921 actgaccgct gaacagcgtg aacgttatct acaggccagc gccgattgcc aaagttccgc
    40981 cccagcaagc ggcattgttt atgaactgtt tgtcgctggt aaaggtaaaa cgtttacagc
    41041 gaaaccgaaa ccggatgcag agatcgcctc gctgttagcg aaacgttcct cttgcggtaa
    41101 agacacgctc tatttctctc tgcgctattt gcccgtcgcg cagagtatcc ttgagtatta
    41161 atgattaaag ggagcaactt agcttgctcc tttccttttc actttcctaa tcaaaatcct
    41221 ggcaagatga attatttagc agaatatctg ttttcatttt attcgtctga cgtgttccaa
    41281 ttatttagca aaaccgcgac aacaaagtga tggtgtaatt tgtaaaaggg aacaaataaa
    41341 tggctcgccc tgcaggattc gaacctgtag cccacggctt agaaactcca ggaacgacat
    41401 tttaagtcaa caaattaccg cgccatctct gcgttcacac gtcccactac ctcaaaatat
    41461 gtaaaacctt gcaagccatt gcgaggcctt atgtgtctca gttttgttcc tcttttttat
    41521 actaaaaaac acagcaattg aggataaacc tcatgctatt atcgctaata tgcatct
//
LOCUS       DACADF010000022        40711 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.022, whole genome shotgun sequence.
ACCESSION   DACADF010000022 DACADF010000000
VERSION     DACADF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.022"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            25..789
                     /locus_tag="ISB30_001154"
     CDS             25..789
                     /locus_tag="ISB30_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988809.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(828..1754)
                     /locus_tag="ISB30_001155"
     CDS             complement(828..1754)
                     /locus_tag="ISB30_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1988810.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1844..2842
                     /locus_tag="ISB30_001156"
     CDS             1844..2842
                     /locus_tag="ISB30_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAP1988811.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2839..3057)
                     /locus_tag="ISB30_001157"
     CDS             complement(2839..3057)
                     /locus_tag="ISB30_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HAP1988812.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKIEGLTQLVQPLKRPL"
     gene            complement(3059..5074)
                     /gene="ligA"
                     /locus_tag="ISB30_001158"
     CDS             complement(3059..5074)
                     /gene="ligA"
                     /locus_tag="ISB30_001158"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP1988813.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHKVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(5145..6143)
                     /gene="zipA"
                     /locus_tag="ISB30_001159"
     CDS             complement(5145..6143)
                     /gene="zipA"
                     /locus_tag="ISB30_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAP1988814.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEVARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6373..7134
                     /gene="cysZ"
                     /locus_tag="ISB30_001160"
     CDS             6373..7134
                     /gene="cysZ"
                     /locus_tag="ISB30_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAP1988815.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7319..8290
                     /gene="cysK"
                     /locus_tag="ISB30_001161"
     CDS             7319..8290
                     /gene="cysK"
                     /locus_tag="ISB30_001161"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP1988816.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8674..8931
                     /gene="ptsH"
                     /locus_tag="ISB30_001162"
     CDS             8674..8931
                     /gene="ptsH"
                     /locus_tag="ISB30_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAP1988817.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8976..10703
                     /gene="ptsI"
                     /locus_tag="ISB30_001163"
     CDS             8976..10703
                     /gene="ptsI"
                     /locus_tag="ISB30_001163"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAP1988818.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIIIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10744..11253
                     /gene="crr"
                     /locus_tag="ISB30_001164"
     CDS             10744..11253
                     /gene="crr"
                     /locus_tag="ISB30_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP1988819.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11296..12147)
                     /gene="pdxK"
                     /locus_tag="ISB30_001165"
                     /gene_synonym="thiJ"
     CDS             complement(11296..12147)
                     /gene="pdxK"
                     /locus_tag="ISB30_001165"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAP1988820.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12252..12620
                     /locus_tag="ISB30_001166"
     CDS             12252..12620
                     /locus_tag="ISB30_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAP1988821.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(12623..13534)
                     /gene="cysM"
                     /locus_tag="ISB30_001167"
     CDS             complement(12623..13534)
                     /gene="cysM"
                     /locus_tag="ISB30_001167"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAP1988822.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(13669..14766)
                     /gene="cysA"
                     /locus_tag="ISB30_001168"
     CDS             complement(13669..14766)
                     /gene="cysA"
                     /locus_tag="ISB30_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAP1988823.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(14756..15631)
                     /gene="cysW"
                     /locus_tag="ISB30_001169"
     CDS             complement(14756..15631)
                     /gene="cysW"
                     /locus_tag="ISB30_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAP1988824.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(15631..16464)
                     /gene="cysT"
                     /locus_tag="ISB30_001170"
     CDS             complement(15631..16464)
                     /gene="cysT"
                     /locus_tag="ISB30_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAP1988825.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16464..17480)
                     /locus_tag="ISB30_001171"
     CDS             complement(16464..17480)
                     /locus_tag="ISB30_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1988826.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(17638..18429)
                     /gene="ucpA"
                     /locus_tag="ISB30_001172"
     CDS             complement(17638..18429)
                     /gene="ucpA"
                     /locus_tag="ISB30_001172"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAP1988827.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            18709..19605
                     /gene="murQ"
                     /locus_tag="ISB30_001173"
     CDS             18709..19605
                     /gene="murQ"
                     /locus_tag="ISB30_001173"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP1988828.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKCLDQHGGFIRKALEKE"
     gene            19609..21033
                     /gene="murP"
                     /locus_tag="ISB30_001174"
     CDS             19609..21033
                     /gene="murP"
                     /locus_tag="ISB30_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAP1988829.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIECMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            complement(21211..22110)
                     /gene="yfeX"
                     /locus_tag="ISB30_001175"
     CDS             complement(21211..22110)
                     /gene="yfeX"
                     /locus_tag="ISB30_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAP1988830.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(22206..22781)
                     /locus_tag="ISB30_001176"
     CDS             complement(22206..22781)
                     /locus_tag="ISB30_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAP1988831.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGNNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(22842..23291)
                     /locus_tag="ISB30_001177"
     CDS             complement(22842..23291)
                     /locus_tag="ISB30_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAP1988832.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(23278..23703)
                     /locus_tag="ISB30_001178"
     CDS             complement(23278..23703)
                     /locus_tag="ISB30_001178"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HAP1988833.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLSPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            23917..24786
                     /gene="amiA"
                     /locus_tag="ISB30_001179"
     CDS             23917..24786
                     /gene="amiA"
                     /locus_tag="ISB30_001179"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAP1988834.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            24790..25689
                     /gene="hemF"
                     /locus_tag="ISB30_001180"
     CDS             24790..25689
                     /gene="hemF"
                     /locus_tag="ISB30_001180"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAP1988835.1"
                     /translation="MKPDAHLVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(25695..26747)
                     /gene="eutR"
                     /locus_tag="ISB30_001181"
     CDS             complement(25695..26747)
                     /gene="eutR"
                     /locus_tag="ISB30_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAP1988836.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGELGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMMTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(26793..27293)
                     /gene="eutK"
                     /locus_tag="ISB30_001182"
     CDS             complement(26793..27293)
                     /gene="eutK"
                     /locus_tag="ISB30_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAP1988837.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(27306..27965)
                     /gene="eutL"
                     /locus_tag="ISB30_001183"
     CDS             complement(27306..27965)
                     /gene="eutL"
                     /locus_tag="ISB30_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAP1988838.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVANIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(27975..28862)
                     /locus_tag="ISB30_001184"
     CDS             complement(27975..28862)
                     /locus_tag="ISB30_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAP1988839.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHHADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(28883..30244)
                     /locus_tag="ISB30_001185"
     CDS             complement(28883..30244)
                     /locus_tag="ISB30_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAP1988840.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCILAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(30256..31659)
                     /gene="eutA"
                     /locus_tag="ISB30_001186"
     CDS             complement(30256..31659)
                     /gene="eutA"
                     /locus_tag="ISB30_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAP1988841.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSRSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGPGTDARSLTGAQL
                     AQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(31656..32882)
                     /gene="eutH"
                     /locus_tag="ISB30_001187"
     CDS             complement(31656..32882)
                     /gene="eutH"
                     /locus_tag="ISB30_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAP1988842.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDASAAKTEAEAQS"
     gene            complement(33099..34286)
                     /gene="eutG"
                     /locus_tag="ISB30_001188"
     CDS             complement(33099..34286)
                     /gene="eutG"
                     /locus_tag="ISB30_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HAP1988843.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAVLTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGQSLPKAVGYGHDLVARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGINKRLADVGATTGHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(34276..35112)
                     /gene="eutJ"
                     /locus_tag="ISB30_001189"
     CDS             complement(34276..35112)
                     /gene="eutJ"
                     /locus_tag="ISB30_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HAP1988844.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAALFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(35123..36526)
                     /locus_tag="ISB30_001190"
     CDS             complement(35123..36526)
                     /locus_tag="ISB30_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HAP1988845.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGPTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNLAIVAAGGPENLLVTVTNPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(36538..36825)
                     /gene="eutN"
                     /locus_tag="ISB30_001191"
     CDS             complement(36538..36825)
                     /gene="eutN"
                     /locus_tag="ISB30_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HAP1988846.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(36932..37225)
                     /gene="eutM"
                     /locus_tag="ISB30_001192"
     CDS             complement(36932..37225)
                     /gene="eutM"
                     /locus_tag="ISB30_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HAP1988847.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(37264..38280)
                     /gene="pta"
                     /locus_tag="ISB30_001193"
     CDS             complement(37264..38280)
                     /gene="pta"
                     /locus_tag="ISB30_001193"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP1988848.1"
                     /translation="MIIKRCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(38277..39080)
                     /gene="eutT"
                     /locus_tag="ISB30_001194"
     CDS             complement(38277..39080)
                     /gene="eutT"
                     /locus_tag="ISB30_001194"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HAP1988849.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGE
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(39077..39778)
                     /gene="eutQ"
                     /locus_tag="ISB30_001195"
     CDS             complement(39077..39778)
                     /gene="eutQ"
                     /locus_tag="ISB30_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HAP1988850.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(39753..40232)
                     /gene="eutP"
                     /locus_tag="ISB30_001196"
     CDS             complement(39753..40232)
                     /gene="eutP"
                     /locus_tag="ISB30_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAP1988851.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(40245..40580)
                     /gene="eutS"
                     /locus_tag="ISB30_001197"
     CDS             complement(40245..40580)
                     /gene="eutS"
                     /locus_tag="ISB30_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAP1988852.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT         9374 a        11416 c        10434 g         9487 t
ORIGIN      
        1 acgatcggca tagggaatta tgctatgaaa aaacatcttt ttactctgac actctcttct
       61 gtattggcga taccagcagt atcccatgca gaatttaaag gcggttttgc tgatataggt
      121 attcattatc ttgactggac tagcagaacc actgagaaat cgtcgaccaa atcacacaaa
      181 gatgattttg gatatctgga actggaaggc ggcgctaact ttagttgggg agaaatgtac
      241 gggttcttcg actgggaaaa cttttataat gatcgtcatg ataaaccggg tagcgagcag
      301 cgttatacat ttaaaaatac caaccgtatt tacctgggtg acaccggatt taatctctac
      361 ttgcatgcgt atggcaccta cggttctgcg aatcgtgtaa atttccatga cgatatgttc
      421 ctttacggga tcggctacaa tttcaccggc agcggttggt ggttcaaacc gttctttgca
      481 aaacgctata cagaccaaac ctattatacc ggcgataacg gttatgtcgc cggttgggtt
      541 gcaggttaca gctttatgct gggcagtgag aaattcaccc tcaccaactg gaacgagtac
      601 gagtttgatc gtgatgccac ctacgcggca ggtaatggcg gcaaagaagg tctgaacggt
      661 gccgtcgcac tttggtggaa tgcaacttca cacatcacca cagggattca gtatcgttat
      721 gcggatgaca aactgggcga agatttttac caggatgcga taatctactc catcaaattt
      781 aatttctgat tgattactgc cggagctccc ggcagtatga attcactcta tatctgatac
      841 aacggatccc cttcccgccc agccattagc gcattgccct ggtctcgtac tacgtcccat
      901 aacgcttcag cggcggtgga aagtgaacgg ttcttcctgc gcaccagcat caactggcgt
      961 tcaaccactg gcgtaatgcg ctttaccacc agtgggctac cttcaggcaa tggtagcgcc
     1021 agtgcgggaa gaatactgat gccgatgcct gcggctacca tcgggaacag cgtcgccgga
     1081 tgtccaatct cctgcacaat attcgcctga ataccgttgc gcgccagcgc tgcgtcaatc
     1141 agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt tcgctccctg taatgcctgc
     1201 caggggacat aatcttccac ggccaaagcg ctatcgcgat ggcaaagcag aaagaaaggc
     1261 tcggaaagaa tcgcttcaca ttgcagatcg cccactgggc caggatcgat gacgatgcca
     1321 aaatcgacat ctccctggcg aatactttcc atcacccact gctgcggacg atcgtgcagg
     1381 acaaactgaa tatctggata gcggcgatgg ctttcagcaa tgcattgcgg aataagatgc
     1441 gcggaaatgg tctggctggc agcgacccga acttttccgc tcagttgttg ccccatacgc
     1501 ccggtatcgc gcaacgtgct gttaagttca tccagcagtc gctcaagacg caaagccaac
     1561 tgctgccctg catctgtaag caccacttca cgcgtggttc tgtccagcag gcgaacacca
     1621 gtatgatttt ccagctcctt cacactgtga ctcactgccg actggctcag gccgatacgc
     1681 tctcctgcac gactaaaact tttctcctgc gctactgtga cgaaaacctt taattgcttt
     1741 aaagaataat tcatctgttt ttttcatgaa tggatgcaat aaatcaattt tatttctcaa
     1801 actggaagac gcacaataga accatcgatc atctggagtc tttatgaaac tttttcgtat
     1861 cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt tctttccggc
     1921 cagaggcgat ttcgtcccct tctttgaaaa tctgaccacc gcagcgatag ccctgctgtt
     1981 ttttatgcac ggcgcgaagt tatcgcgtga ggcgattatt gctggcggtg gccactggcg
     2041 actgcatttg tgggtaatgt gcagcacctt cgtgctgttt ccgattctgg gcgtgctgtt
     2101 tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac tccggttttc tctacttgtg
     2161 cattctcccc gcgaccgtgc agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt
     2221 cgcggcggcg gtttgttctg cgtcagcatc cagcctgctg gggattttcc tttcaccatt
     2281 gctggttggt ctggtgatga atgttcacgg tgcagggggc agccttgagc aggtcggtaa
     2341 aattatgctg caactgttgc tgccatttgt gttggggcat ctttcccggc cgtggattgg
     2401 tgactgggtg tcgcgtaata aaaaatggat tgcgaaaact gaccagacgt ccattctgtt
     2461 ggtagtttat acggcattca gcgaagccgt cgttaacggt atctggcaca aagttggctg
     2521 gggatcattg ctgtttatcg tggtggtcag ttgcgttctt ctggctatcg tgattgtagt
     2581 taacgtcttt atggcacgcc gactgggctt caataaggca gatgaaatta ctatcgtctt
     2641 ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg gcaaacattc tgttccccac
     2701 atcggtgatc ggtatgatgg tgctgcccct gatgattttc catcagatcc aattgatggt
     2761 ctgtgcggtg ctggcgcgtc gatacaaacg ccagaccgaa cagttacagg cgcagcagga
     2821 aagcagcgcc gataaagctt aaagcggacg tttcaggggc tggaccagtt gcgtcagccc
     2881 ctcgatttta atcagcagcg tgatttgcat tagctcaccg agcttccctg ccgggaactc
     2941 atctttgcgg gcaaaccaca gcagatactc ctccggcagg tcgattaagc gacgcccttt
     3001 gtacttgcca aacggcatta tcgtattggc tatttcaatc agctgctctt tttccatctc
     3061 agctacccag caaacgcagc atttccgttt cgtcgatgac ttcaataccc agttcctgcg
     3121 ccttcgccag tttagatcct gcagcttcac ccgctatcac cagatcggtt ttcttcgaca
     3181 cgctgcccgc gactttcgcc cccagttcga ccagtcgagc tttagcgtca tcacgcgaca
     3241 tctggcttaa gctgcccgta agcaccacgg ttttaccagc aaacggactg tcaatctctt
     3301 ccgcattgat aacgatcggc tcaggccagt gaacaccttc cgccaacagc tcgctgatga
     3361 cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg ggaggcaacg acaattccaa
     3421 catcaggcac cttttgcagc tcttcaatcg acgcggcttc cagcgcttcc agcgtgccga
     3481 aatatgccgc cagacctgct gcggtagcct cgccgacttc acggatgcca agtgcataga
     3541 ggaagcgggc aaaggtggtt tctttcgctt tttccagcgc gttaaccacg ttttgtgccg
     3601 atttcggccc catacgctcc agtccggtca gtttgcctgc ggtgagtttg aacagatccg
     3661 ccggagtgtg gacatattct ttttcaacca gttgatcgat gattttgtcg cccattccgt
     3721 caacatccat cgcacggcgg gaaacaaagt gtttcagaga ctctttacgc tgcgcaccgc
     3781 aaatcaggcc accggtacag cgggcaaccg cttcaccttc cacacgctcg acgtcagaac
     3841 cacataccgg acaatgcgtc gggaatacaa cctcacgggt atcttccggg cgttcagaaa
     3901 gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg gcgaataacc actttatcgc
     3961 caatgcgtaa accaagacgt tcgatttcgt cagcattatg cagtgtagcg ttactcacca
     4021 acacgccagc aacatggaca ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc
     4081 caacctgaaa ctcgacgtca cgcacaaagg tcatctgttc ctgcgccggg aatttaaacg
     4141 ctaccgccca gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt gccagtgagt
     4201 tgaccttaat caccacgccg tcgatatcaa agcccagcgt cggacggtct ttttctactt
     4261 tgtgataaaa cgccagcact tcttccgccg attcacaaag tgttacccga tcactgacag
     4321 gtaatcccca tgctttaaat tgcattaagc gtcccaaatg ggtatccggc aattcaccgc
     4381 cttccagaac accaacgccg tagcagaaga aagtgagcgg tcgcttcgct gtaatacgcg
     4441 gatcaagctg acgcagtgaa ccagctgccg cattacgtgg gttagcaaac actttcccgc
     4501 ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg aacacttcac
     4561 cacgcacttc cagacgcgcc gggatattct caccgtgtag cttcagcgga atggcgcgaa
     4621 tagtacgcac attagacgtg atatcttccc cggtagtgcc atcgccacga gtcgcggcac
     4681 tgactaaaac gccattttca tacagaatac tgacggcaag accatccagc ttcagctcac
     4741 agcaccaggt gactttctcg ttgcttttca gacggtcctg cacacgtttg ttgaaagcaa
     4801 gaaagctttc ttcatcaaaa acgttatcca gcgacagcat tggcacttca tggcggatct
     4861 ggctgaaagc cgccagcggc gctgcaccta cacgttgggt aggcgaatca ggcgtaatca
     4921 gttctggatg tttggtttcc agctcgcgca gttcgcgcat cagcctgtcg tattcagcgt
     4981 cgggaatttc cggcgcatcc atcacatgat aaagatattc atgatggcga agcgtcgttc
     5041 gcagttctgt cagttgttgt tcgattgatt ccatatcgca ccatcaatgc taaaaacccc
     5101 cgacaagcgg gggttcgaaa aggagttaat ttgccttaag tgtatcaggc gttggcgtct
     5161 ttgacttcgc ggatgatgtc ctggtactcg cgcaatttct gcggagtcat catacggcgc
     5221 tgatcgtcaa gcactacacc acccacttca tcggcaatat gctgcgcaga ttgcagcatc
     5281 agcttgaagt tctgcagctc gtcaccgtaa gacggtacct gcataaagat agtgacaccc
     5341 ggagtagtga aatccttcat ttcaggatca aaggttcccg gtttcaccat attcgccagg
     5401 ctgaataacg ccgggccgct gccatccggg ctaagatgac gatggtaaat gttcatatca
     5461 ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt taactcgcta
     5521 ccgtgatgcg ccgcgacgtt catgataatc accgcttctt tgcgcttcgg tttatccata
     5581 actggagccg gttccgcaac aggctcaggc tgtggtgccg ctacgggttc tgcaggctgg
     5641 aaagcctgtt gtgccggttg cggtgctgaa tgcactggct gcggcgctga cgcgacctgt
     5701 ggcgaaactg gctgctgcaa cggctgttca ggctgcggct gataggcagg ctgctgcacg
     5761 ggttgctgca ccggctgcgt tggacgcgga gcatgttgcg gcggtacctg cgcttcaggc
     5821 ggctgctgga ccggttgacg cggctgcgca gacgcataag gcggttggta ctggtgttgc
     5881 ggcgacggac gagcaacctc atgctcctgg gcgttagccg gggcatgatt cacgcggtga
     5941 acacgaacct caccaacgcc ctcatcatct tcgacatcct cgtcataaga atcgtcgtca
     6001 cgttttgact tcattcgttt taatggccga tcgcggaaca tagaagatcg ttctttacgg
     6061 ctggtccaga aaccatgtac cagtaaagcg attatggcga tcgcgccaac aatgattaat
     6121 atcagacgca aatcctgcat cattatattc tctgttgttc taacaccttg ccaccacggc
     6181 aaacatttac tcactaagag tatttgccga ttacctcaag tgcaagtgca ctattaactt
     6241 tcacagcaca aagatagatg aaatcgtgct ttttgctgtt ttttcgaaca tatcctaact
     6301 gtccattgcg caattacccg gtaaaatacg cagaattttc ctgggattgg tcaaaaggag
     6361 ctcatcctga ttatggtttc atcattcaca tctgccccac gcagcggttt ttactatttt
     6421 gcgcaaggct ggaagctcgt ctcgcaacct gggattcggc gtttcgttat tttaccgctg
     6481 ctggtcaata ttttgttgat ggggggcgca ttctggtggc tctttacgca actcgatgtc
     6541 tggatcccga ctctcatgag ttatgttccg gactggttac aatggctgag ctatctgttg
     6601 tggcccctgg ctgtcatctc tgtgctgtta gtgtttggct atttcttctc aacgattgct
     6661 aactggattg ctgctccgtt caacggttta ttggctgaac aactggaagc aagactgact
     6721 ggcgctacac cgccagatac cggaattttc ggtatcatga aagatgtgcc gcgaatcatg
     6781 aaacgcgaat ggcaaaaatt tgcctggtat ctgccgcgcg caattgtatt gctaattctt
     6841 tacttcatcc ctggcattgg gcaaaccgtc gcgccggtac tgtggttcct gtttagcgcc
     6901 tggatgttag ccatccagta ttgtgattac cccttcgata accacaaagt gccgtttaaa
     6961 gagatgcgca ccgccctgcg cacacgcaaa atcaccaata tgcagtttgg tgctttaacc
     7021 agcctgttta cgatgatccc gctgcttaat ctgttcatta tgcccgttgc cgtttgtggc
     7081 gcgacggcga tgtgggttga ttgctatcgc gataaacacg ccatgtggcg gtaacaatct
     7141 accggttatt ttgtaaaccg tttgtgtgaa acaggggtgg cttatgccgc cccttattcc
     7201 atcttgcatg tcattatttc ccttctgcat atagatatgc taaatcctta cttccgcata
     7261 ttctctgagc gggtatgcta cctgttgtat cccaatttca tacagttaag gacaggccat
     7321 gagtaagatt tttgaagata actcgctgac tatcggtcac acgccgctgg ttcgcctgaa
     7381 tcgcatcggt aacggacgca ttctggcgaa ggtggaatct cgtaacccca gcttcagcgt
     7441 taagtgccgt atcggtgcca acatgatttg ggatgccgaa aagcgcggcg tgctgaaacc
     7501 aggcgttgaa ctggttgaac caaccagcgg taataccggg attgcactgg cctatgtagc
     7561 tgccgctcgc ggttacaaac ttaccctgac catgccagaa accatgagta ttgaacgccg
     7621 caagctgctg aaagcgttag gtgcaaacct ggtgctgacc gaaggtgcta aaggcatgaa
     7681 aggcgcaatc caaaaagcag aagaaattgt cgccagcaat ccagagaaat acctgctgct
     7741 gcaacaattc agcaatccgg caaaccctga aattcatgaa aaaaccaccg gtccggagat
     7801 ctgggaagat accgacggtc aggttgatgt atttattgct ggcgttggga ctggcggtac
     7861 gctgactggc gtcagccgct acattaaagg caccaaaggc aagaccgatc ttatctctgt
     7921 cgccgttgag ccaaccgatt ctccagttat cgcccaggcg ctggcaggtg aagagattaa
     7981 acctggtccg cataaaattc agggtattgg cgctggtttt atcccggcta accttgatct
     8041 caagctggtc gacaaagtca ttggcatcac caatgaagaa gccatttcta ccgcacgtcg
     8101 tctgatggaa gaagaaggca ttcttgctgg tatctcttct ggagcagctg ttgctgcggc
     8161 gttgaaacta caagaagatg aaagctttac caataagaat attgtggtta ttctgccgtc
     8221 atcgggtgag cgttatttaa gcactgcatt gtttgccgat ctcttcactg agaaagaatt
     8281 gcaacagtaa tgccagcttg taaaaaatgc gtaaaaaagc acctttttag gtgctttttt
     8341 gtggcctgct tcaaactttc gcccctcctg gcattgattc agcctgtcgg aactggtatt
     8401 taaccagact aattattttg atgcgcgaaa ttaatcgtta caggaaaagc caaagctgaa
     8461 tcgattttat gatttggttc aattcttcct ttagcggcat aatgtttaat gacgtacgaa
     8521 acgtcagcgg tcaacacccg ccagcaatgg actgtattac gctcttcgtg cgtcgcgtct
     8581 gttaaaaact ggcgctaaca atacaggcta aagtcgaacc gccaggctag actttagttc
     8641 cacaacacta aacctataag ttggggaaat acaatgttcc agcaagaagt taccattacc
     8701 gctccgaacg gtctgcacac ccgccctgct gcccagtttg taaaagaagc taagggcttc
     8761 acttctgaaa ttactgtgac ttccaacggc aaaagcgcca gcgcgaaaag cctgtttaaa
     8821 ctgcagactc tgggcctgac tcaaggtacc gttgtgacta tctccgcaga aggcgaagac
     8881 gagcagaaag cggttgaaca tctggttaaa ctgatggcgg aactcgagta atttcccggg
     8941 ttcttttaaa aatcagtcac aagtaaggta gggttatgat ttcaggcatt ttagcatccc
     9001 cgggtatcgc tttcggtaaa gctctgcttc tgaaagaaga cgaaattatc attgaccgga
     9061 aaaaaatttc tgccgaccag gttgatcagg aagttgaacg ttttctgagc ggtcgtgcca
     9121 aggcatcagc ccagctggaa acgatcaaaa cgaaagctgg tgaaacgttc ggtgaagaaa
     9181 aagaagccat ctttgaaggg catattatgc tgctcgaaga tgaggagctg gagcaggaaa
     9241 tcatagccct gattaaagat aagcacatga cagctgacgc agctgctcat gaagttatcg
     9301 aaggtcaggc ttctgccctg gaagagctgg atgatgaata cctgaaagaa cgtgcggctg
     9361 acgtacgtga tatcggtaag cgcctgctgc gcaacatcct gggcctgaag attatcgacc
     9421 tgagcgccat tcaggatgaa gtcattctgg ttgccgctga cctgacgccg tccgaaaccg
     9481 cacagctgaa cctgaagaag gtgctgggtt tcatcaccga cgcgggtggc cgtacttccc
     9541 acacctctat catggcgcgt tctctggaac tgcctgctat cgtgggtacc ggtagcgtca
     9601 cctctcaggt gaaaaatgac gactatctga ttctggatgc cgtaaataat caggtttacg
     9661 tcaatccaac caacgaagtt attgataaaa tgcgcgctgt tcaggagcaa gtggcttctg
     9721 aaaaagcaga gcttgctaaa ctgaaagatc tgccagctat tacgctggac ggtcaccagg
     9781 tagaagtgtg cgctaacatt ggtacggttc gtgacgttga aggtgcagag cgtaacggcg
     9841 ctgaaggcgt tggtctgtat cgtactgagt tcctgttcat ggaccgcgac gcgttgccca
     9901 ctgaagaaga acagtttgct gcttacaaag cagtggctga agcgtgtggc tcgcaggcgg
     9961 ttatcgttcg taccatggac atcggcggcg acaaagagct gccatacatg aacttcccga
    10021 aagaagagaa cccgttcctc ggctggcgcg ctatccgtat cgcgatggat cgtaaagaga
    10081 tcctgcgcga tcagctccgt gctatcctgc gtgcctctgc tttcggtaaa ttgcgcatca
    10141 tgttcccgat gatcatctct gttgaagaag tgcgtgcact gcgcaaagag atcgaaatct
    10201 acaaacagga actgcgcgac gaaggtaaag cgtttgacga gtcaattgaa atcggcgtaa
    10261 tggtggaaac accggctgcc gcaacaattg cacgtcattt agccaaagaa gttgatttct
    10321 ttagtatcgg caccaatgat ttaacgcagt acactctggc agttgaccgt ggtaatgata
    10381 tgatttcaca cctttaccag ccaatgtcac cgtccgtgct gaacttgatc aagcaagtta
    10441 ttgatgcttc tcatgctgaa ggcaaatgga ctggcatgtg tggtgagctt gctggcgatg
    10501 aacgtgctac acttctgttg ctggggatgg gtctggacga attctctatg agcgccattt
    10561 ctatcccgcg cattaagaag attatccgta acacgaactt cgaagatgcg aaggtgttag
    10621 cagagcaggc tcttgctcaa ccgacaacgg acgagttaat gacgctggtt aacaagttca
    10681 ttgaagaaaa aacaatctgc taatccacga gatgcggccc aatttactgc ttaggagaag
    10741 atcatgggtt tgttcgataa actgaaatct ctggtttccg acgacaagaa ggataccgga
    10801 actattgaga tcattgctcc gctctctggc gagatcgtca atatcgaaga cgtgccggat
    10861 gtcgtttttg cggaaaaaat cgttggtgat ggcattgcta tcaaaccaac gggtaacaaa
    10921 atggtcgcgc ctgtagacgg caccattggt aaaatctttg aaaccaacca cgcgttctct
    10981 atcgaatctg atagcggcgt tgagctgttc gtccacttcg gtatcgacac cgttgaactg
    11041 aaaggcgaag gcttcaagcg tattgctgaa gaaggtcagc gcgtgaaagt tggtgatact
    11101 gtcattgaat ttgatctgcc gctgctggaa gagaaagcca agtctaccct gactccggtt
    11161 gttatctcca acatggacga aatcaaagaa ctgatcaaac tgtccggtag cgtaaccgta
    11221 ggtgaaaccc cggttatccg catcaagaag taattcttgc cgcagtgaaa aatggcgccc
    11281 atcggcgcca tttttttatg cttcagccag aggcggcaaa atcaattcat cgctctcatg
    11341 ctgctgggta tagcgcatca cttccagtac gcgcaacccc gcgcggtgca ccgcatcggt
    11401 taacgccttc cctttcagca aaccactgat aagctgagca caaaacaggt cgccagtccc
    11461 tttcaggtca gttttaaccc gggaatggga aatgacattc acgctgtcgg cactgaccac
    11521 cacaacctgc atctcctgat tttcttcatt gccggaggcg ctggtaatca ccacccactt
    11581 taatgtgtct gaaagcagac tttttgcggc agcaatggca ctgtcgagat cgcggcaatc
    11641 tttaccggtc aggatttcca gctcaaagat attgggggta attccctgtg ccagcggcag
    11701 taaatattgt cgatacgcct cgggaaggtc aggtttgaca taaattccgc tatcaatatc
    11761 gccaatcacc ggatcgacca tgatcaatag gtcaggatgg tctttgcgta gcgcagtcag
    11821 ccactcggca aggattttga tttgcgatgc cgttcccata tagcccgtgg ttacagcacg
    11881 aagttggcgc agcgcatcac gctcctgaag cgcacgcaaa taaccgctaa accattcatc
    11941 cggaatcgca ccaccgtaga aagtgtcata atgcggcgta ttgctcagca ataccgtcgg
    12001 cacggcaaag acattcaggc cgttctgctt tatagcaggc accgcaatgc tgttgcctac
    12061 gctgccgtac actacctgcg actgcacggc gacgatatcc gcctgcagtg ccctgctctt
    12121 atcgttaaac aacaacaaac tactcattta attttttctc cttgccgatg atcctcatcg
    12181 tattccagcc gaaactttac ctgattctgg cagtcaaatc ggctattaca aaacaaggat
    12241 aaggtaattc agtgaagaaa atcatttgtc tggtcgttac gctgctaatg acactccccg
    12301 cttatgcgaa gttaacggcc catgaagaag cccgtatcaa cgccatgctg gagggattag
    12361 cacagaaaaa ggatttgata tttgtgcgca acggtgatga acatacctgc gatgaagcgg
    12421 tttctcatct gcgcctgaag ctgggcaata cccgtaatcg cattgataca gccgagcagt
    12481 ttattgacaa ggtggcctcg tcgtcatcga ttaccgggaa gccgtatatc gtgaagatgc
    12541 ccggtaagag cgatgagaac gcacagcctt ttttacatgc gttaattgcg cagacggata
    12601 aaacggtgcc tgcgcaataa tcttaaatcc ccgccccctg gctaaaatgc tcttccccaa
    12661 acaccccggt agaaaggtag cgatcgccac gatcgcagat gatcgccacc accaccgcgc
    12721 cagggttagc ttttgccacc cgcagtgctc cggcaaccgc gccgccggag cttacgccgc
    12781 agaaaatccc ttcccgcact gccagttcgc gcatggtgtt ttccgcatcg cgctgatgaa
    12841 tatccagcac ctcatccacc agagaagcat tgaaaatccc cggcagatat tccgcaggcc
    12901 agcggcgaat gccgggaata ctgctgcctt cttccggctg caggccaaca atggtcaccg
    12961 gtttggattg ttcgcgcata aagcgtgaca cgccggtgat agtgccggtt gttcccatgc
    13021 tggagacaaa atgggtaata cgcccgccag tttgctgcca gatttccggc ccggtagtgg
    13081 tgtaatgggc gtaagggtta tcgggattat tgaactgatc gagcaacttt ccttcgccgc
    13141 gattcgccat ctccagcgcc agatcgcgtg cgccttccat gccctgttct ttggtgacca
    13201 gaatcagttc tgcaccgtag gcacgcatcg ccgcacggcg ttcctggctc atgttgtcgg
    13261 gcatcagcaa tttcatgcga tagcctttaa gcgccgcaat cattgccagc gcaatgccgg
    13321 tgttaccact ggtggcttcg atcagcacat cacccggttt aatctcgccg cgcttttccg
    13381 cctcgacaat catcgaaagc gccgcacggt cttttaccga tcccgccggg ttattgcctt
    13441 ccagttttaa ccacacttca ctgccgttat ccggcgccat tcgctgcaac ttcaccagag
    13501 gcgtattgcc tattgtttgt tctaatgtac tcacgatctc tgtccatacg tggtgttgcc
    13561 tgatgcgacg cttacgcgtc ttatcagacc tacaggttac aaaaccttgc cattaaaaaa
    13621 cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg
    13681 tgcgagagca agttcctcat cgcgggtttc aatacgctca tcgccgttat acagccgcgc
    13741 atgttgcaga ccaacgtata aacgatcgcc acgctgcggg gcatcgtcgc catgcatcac
    13801 gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc
    13861 tttcgggctg gcttccagta cctgtacggg tagcggcgaa tcgaggctgg tacggcggct
    13921 gatatccact tcccacgggc gcaggaagag atccaccggc ccctgatacg cgggggtgta
    13981 tcccagcggc cagcgatgtg cgccaacgtg gaactgcccg ccgcgaatgg ttccctgcag
    14041 gcggtttacc tcgcccataa actccagcac aaaacgagtc gccggttcac gccatacctg
    14101 atccggtgcg tcagcttgtt caatattgcc ctgactcatc accactacac gatcagctac
    14161 ttcggtcgcc tcttcctggt cgtgggtcac gaacacgctg gtgaatttca gttcttcatg
    14221 cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg
    14281 ttcatcaagc agcaaaattt gcggttccac agccagcgcg cgtgcaagcg ccacacgctg
    14341 tttctgaccg ccggaaagct gcgccggata acgatccgcc agatgagcaa gctgcaccat
    14401 ctccagcaat tttgtcactt tcgctttgat cgccgccgca ttcgggcgtt cacgccgtgg
    14461 caacaccgtc aggccaaaag cgatattgtc aaacaccgtc atatggcgaa acagcgcgta
    14521 atgctggaac acgaaaccga ctttacggtc ccgcgcgtgc agacggctca cgtcggtacc
    14581 gtgaaagcga atatgcccac tggtttgatg ctccagcccg gcgataatgc gcagcagcgt
    14641 agtttttccg gaaccggacg gcccaagcaa cgcgaccatc tgaccagaag gaatatccag
    14701 tgagatatcg ttcagcacct gggtgcgacc aaaagacttc ttaatattgg caatctcaat
    14761 gctcatgatg ttcctcctgc tgcgcgcgtt tttcctgatt ttccaggcgc cactgcaaca
    14821 tactctttaa aaacagggtg ataatcgcca tcagcgtcaa caacgcggcg gcggtaaagg
    14881 agccgacggt gttgtagtcc tgctccagca attcaatctg taacggcagc gacagggttt
    14941 cgccacgaat cgagccggaa accaccgata ccgcgccaaa ctcgccaatc gcgcgggcgt
    15001 tggtcagcac cacgccgtaa agcagcgccc agcggatgtt cggcagcgtg acgcgacgga
    15061 acatctgcca gccggacgcg ccaagtaaaa tcgccgcttc gtcttcctgg ctgccctgac
    15121 ttaacattac cggtaccagt tcgcgcacca caaacggaca cgtcacgaag atggtgacca
    15181 gcaccatacc cggccaggag aacataattt gcagattatg ttcatccagc cagccgccca
    15241 gcgggccgtt agagccgtag aacagcagat acaccagacc agcaaccacc ggcgacacgg
    15301 caaacggaat gtccagcagc gtcagcaata gctggcgtcc ggggaagtta aagcgcgtca
    15361 ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca
    15421 tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattttgt aacaccggca
    15481 tcagtccttt gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg
    15541 ccgataccag catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt
    15601 cataacgctt caattgtgtc acttccgcca ttaatgacct accacacgcc gaccaaagcg
    15661 actttgcaga gtgttaattg agaacagcaa cagcagcgat gccgcgagga tcaccgaggc
    15721 aatcgcgctc gccgccgggt aatcaaactc ctgtaagcga acaaagatca tcagcgacgt
    15781 cacttctgtt ttccaggcga tattcccggc gataaaaatc accgcgccaa actcaccgag
    15841 gctgcgggta aacgacagcg ccacacccgc caccagtgcc ggagatagtt ccggcagcac
    15901 cactttgcga aaactctgcc agcgtgttgc gccgagcgtt tctgccgcct cttcatattc
    15961 cgggcctaac tcttccagca ccggctgcac ggtgcgcacc acaaacggaa tgctggtaaa
    16021 ggccatcgcc accgcaatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag
    16081 ccattcaccg taaaaaccgt tcacggaaaa gagcgaggcc agcgttaaac cggcgactgc
    16141 cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcgac ccggaaagcg
    16201 atagcgggtt aagatccacg ccatcagcag accgaaaacg ccgttaaaaa tcgatgccac
    16261 aaacgccgac agcagcgtca ctttataggc tgcgaccacc tgcgggttgg tgatcacctc
    16321 ccagtactgc gcccagctca tctgggccag ttgcatcacc agcgcggaga gcggcagcag
    16381 caaaatcagg cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac
    16441 gcgtcttgaa gaaactgcaa acattactta cgccccgccg ctaacagctt gtctaactcg
    16501 ccgccgctgg tgaagtgggt tttcatcact tccggccagg agccaaattt atcttccacg
    16561 cggaacagct cggtctgcgg gaatttatct ttcagtttgc ccatgacttc cgggttattt
    16621 acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg ggctgtagag ccagttcagg
    16681 taggcttttg ccgctttttc cgtgccgttg gcctgtacgt ttttatccac ccacgccacc
    16741 gggaattccg ccagaatgtt ggttttcgga atcaccactt caaagccctg cgcttcatac
    16801 tgtttacgga tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc
    16861 tcggcaaaag tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg
    16921 aactgggtca taaactgttc ggttttggct ttatcgccac cgtcggcttt atccgctgca
    16981 ccccacgcgg ctaaataggt gtaacgcgcg ttaccggagg ttttcgggtt cggaaaaatc
    17041 agcttcacgt cggagcgcac cagatcgttc cagtcgtgga tattcttcgg gttaccctta
    17101 cgcaccagga agcccatggt ggagtagaac ggcgagctgt tgtttggcag acgcgactgc
    17161 cagtcagccg gaataagctt gcctttgtcg tgcaggatct gcacgtctgt cacctggtta
    17221 taagtgacaa cgtcggcttt taagccctgc aaaatcgcca gcgcctgttt tgatgacccg
    17281 gcatgagatt gttttatcgt cagtttgtcg ccgccgttat ctttggccca ttgctgttca
    17341 aacggcgggt tcagggcggc aaacagctcg cgcgagacgt cataagaact gttcagcagt
    17401 tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcacgag tgagttcttt
    17461 ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct aatagccatc
    17521 atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt
    17581 tgaaaaggga ataagacgaa atcctcccca caaatgcagg gaggaaacag aggtgaatca
    17641 gatgccgacg ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc
    17701 tgttaaatag ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccagcgg
    17761 atcggcgagg cgacgcatcg ggattgcttt cgccatttca gtcagcaccg attctggatc
    17821 ttccgggttc gactggcggg caatgctttc cgccattggc gtgcgaacgt atccagggca
    17881 gatggcgtta acgcgaatac cagactgcgc gtactccacc gccagcgatt tagtcaggcc
    17941 aacaatcgcc gctttcgtta aggcgtacgc cgtttcgccc gggtctgcca ccatatcgcc
    18001 agtgactgaa gacatcatca caatgcggcc atctttgcgg gcaatcatct ccggcagcac
    18061 cgccttcgtg acgttccata cgccttttat attgatatca atatggaaat cgcgatcttc
    18121 atcgctcata tcgaggaagc tgcccagacg acaaacgcct gcgttattca ccagaatatc
    18181 aatgcgtcct tctttttcct tagcgcgctt aatagctgcg gctaccgacg ccgggtcacg
    18241 cacatcggcg acaaccgccg tacagcgatg accacgacca cacagttcgt ccgccagctt
    18301 ttcgatctca ggggagatat ccagcaagat taggtttgcg ccatgacgtg caaaagttct
    18361 ggcaattcct tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag
    18421 tttacccatt ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa
    18481 atacaggata aacaaaatat tgctgcggat cagctcacta attcaggaaa atctcaattt
    18541 agtcgctgct ttatgcagca cctcaagcgt tacgtcagcg cgcaatcatt ttcagtgaca
    18601 tcacatgagt aatattcgga cttaatgatt cctttgtgtc tatcaaatcg ggatatatga
    18661 tcccatttat ctgcaattat ttgatcgcaa atattaaggt caccaccgat gcaacttgaa
    18721 aagatgatta ccgaaggctc gaacgccgcc tcggctgaaa ttgaccgcgt atcaacgctg
    18781 gaaatgtgcc ggattatcaa cgatgaagat aaaaccgtac cactcgccgt tgagcgcgta
    18841 ctgccggata tcgccgcggc gatcgatgtt atccacaccc aggtaagcgg cggcggccgt
    18901 ctgatttatc tcggtgcggg aacatccggt cgtctgggga ttctggatgc cagcgaatgt
    18961 ccgccgacct acggcgtgaa accgggcctg gtagttggtt tgattgctgg cggcgaatat
    19021 gccattcagc acgcggtgga aggcgcagaa gatagccggg aaggtggtgt taatgatctg
    19081 aaaaatatcg gcttaaccgc gcaggatgtg gtggttggta ttgcggccag cggacgcacg
    19141 ccgtatgtga ttgccgggct ggaatacgcg cgccaactcg gctgccgtac agtgggcatt
    19201 tcctgtaatc cggggagtgc cgtttcatcc accgcagagt ttgctattac gccggtagtt
    19261 ggtgccgaag ttgttaccgg ttcttcgcga atgaaagcag gcacggcgca gaaactggtg
    19321 ctcaacatgc tctctaccgg gctgatgatt aaatccggca aagtgttcgg caacctgatg
    19381 gtcgatgtgg tcgccaccaa cgaaaaactg catgtgcgcc aggtcaatat cgtcaaaaac
    19441 gctaccggat gtaatgccga acaagcggaa gcggcgttaa ttgcttgcga gcgcaactgt
    19501 aaaacggcca tagtgatggt actgaaaaat ctcgatgccg ctgaagctaa aaaatgcctg
    19561 gatcaacacg gcggttttat tcgcaaggct ctggaaaagg aataacccat ggctaaagag
    19621 atcagcagtg aacttctaaa caccattctt acccgggtcg gcggaccggg aaatatcgcc
    19681 agttgtggta actgtatgac gcgcctgcgt ctgggtgtac atgacagttc actggttgat
    19741 cccaatatca aaacgctgga aggcgtgaag ggggtcattt tgaccagcga tcaggtgcag
    19801 gtggtttttg ggccaggcaa agcgcatcgt gccgcaaaag ccatgagcga gctgctgggc
    19861 gaggcaccgg tacaggatgc cgcagaaatc gccgcccaga ataaacgcca gttaaaagcc
    19921 agacaaactt ccggcgtgca acaatttctc gccaaattcg ccaccatctt cacgccgctg
    19981 atccccggtt ttattgccgc cggtctgctg ctggggatag cgacgttaat tgccacggtg
    20041 atgcacgttc cggcagatgc tcagggcacg ctacccgatg cgctgaattt tatgaaggtg
    20101 ttcagcaaag gcctgttcac cttcctggta attctggtgg gctataacgc ggcccaggca
    20161 tttggtggca cgggcgtaaa tggcgcaatt atcgctgcgc tctttttgct cggttataac
    20221 cccgctgcaa ccacgggtta ctacgccgga tttcacgatt tctttggtct gcctatcgat
    20281 ccgcgcggca atattatcgg cgtgttgatt gccgcatggg cctgcgcccg catcgaatgc
    20341 atggtgcgcc gctttatgcc ggacgacctc gacatgctgt tgacctcatt aatcaccctg
    20401 ctgatcaccg ctacgcttgc gtacctgatc attatgccgc tgggcggctg gctgtttgaa
    20461 ggtatgtcat ggctgtttat gcacctgaac agtaatccgc tcggttgtgc ggttttagcc
    20521 gggctgttcc tgatcgccgt ggtatttggc gtgcatcagg gctttattcc tgtttatctg
    20581 gcgttaatgg acagccaggg attcaacagc ttatttccca tcctttcaat ggcaggcgca
    20641 ggccaggtgg gcgcggcgct ggcgctctac tggcgggcgc aaccgcacag tgcattacgc
    20701 agtcaggtcc gcggggcgat tattcccggt ctgcttggcg ttggcgaacc gctgatttac
    20761 ggtgtcaccc tgccccgcat gaaaccgttt gttaccgcct gtttaggcgg cgcggcgggc
    20821 ggtttgttta tcggcctgat agcctggtgg ggtctgccga tgggcttaaa cagcgccttc
    20881 gggccgtctg gtctggtggc gctgccgctg atgacttccg cacaaggcat ccttccggca
    20941 atggcggttt acgctggcgg tattctggtg gcatgggtta gtgggtttat tttcaccaca
    21001 ctctttggct gccgtaacgt caatctggac tgattatgaa acggacaatg ctctatcttt
    21061 ctctgctggc tattagctgt agcgtcagcg ccgcaaaata ccctgttttg gcagaaagta
    21121 gttgatcaag tgtatgcggc gtcaaagcag taataaaaag gccagtcagt tgtgttactg
    21181 gctggccttt aatcaatgaa tcagaaacgc ttacagcgcc atcagcttgt ccagcgacgg
    21241 tgcgaagtaa tagccgccag tgaccggttt ggtgaaacgc aacatcgcat cacgcttacc
    21301 atcggtatcg ccaaacatgc tcagcagttg ctgctcaatg ttatgcagac gcgcgcagta
    21361 ggcgcagaag tacagaccgt gagtgccact ggcggtgccg tacggcaggc tctgacgaac
    21421 aatcttcagc cctttgccat cttctttcag atcaacgcgg gtcaggtgag aggtttccgg
    21481 acgttcgtcg ccgtcaatct cttcgttggc ctctttggtg cgcccaatca tcatctcttg
    21541 atcgtgaacg ctcatccgat tgagctgctt caggttgtgc tcccaacgct gcacaaacac
    21601 atagctgccg cccgcatcca cgccgtcttt gataacagcc acttcgcggc gcgtctcttc
    21661 acccgccggg ttttctgtac cgtcaacaaa gccgctcagg tcacgctctt caacccaacg
    21721 gaagccatgg atctcttctt tcacttcaat gcagtcacca aaggcttcca tcgccgcctg
    21781 agcgacagag aagttcacgt cgtgacgcag agaaagaatg tggatcaaca catcgaactg
    21841 ggtcgtcggt gcaagacctt taccgtagcc cggaaaatct ttcagctctt cggccccaac
    21901 gccgccgctc agagcgcgcc aggtattgtt accaaaggca accaccgcac caagatgcgc
    21961 gtccgggaat ttcgcttcaa aagttgccag tttgtcggca aatgttttac tggccgcacg
    22021 cagggcgtca acttcccctt tcacgttggc ttcgatccaa atcgccgcgc ggcaatgttc
    22081 tggcaaaatg ccactctgaa cctgagacat tgttcctcct gaaaataata atgccacgca
    22141 atccgtggca ttgttgggcg ctattttacg tgttttttcg cggcggcggt ttgttcagac
    22201 gcaaattaac gccgccagat aattttactg actttccagt ttttcagggt atcgtccgaa
    22261 ggcattaacc cttcaggacc actccattcc ccgctgaact ggtagctaat atgttgactg
    22321 ccttcggctt tacattcgac ggcacgatta tcatcactgt cggccttttg gcaattgcca
    22381 aatgctttgc tgtaaaggtc actaaacggt gtaccgattt taacaccggt gtcagcagga
    22441 atatcgctat ccagcacatc aatgcggctg atcgtgcctt gatcgccgtt aatcaccatc
    22501 gccacgttgt tgcctttcat cacttcaaaa aagcgcacca cgttgccgtt cgcggttttc
    22561 attccgctgc gcaggcgata atcgccatca agcgcatcag caatggcttg ttcttgcagt
    22621 ggtgtggacg ccgttaattc acccacgccc tgctcgctca ctttggtgga tgatccaaac
    22681 cagttccacg ggttagcggc cgaccagtta actgacgaca tcgtggaaca gccggtcagc
    22741 atcaacggca tagcgcataa cattaaacgc agcgatttca tgtcacttcc tttggttatt
    22801 caataacgtt gcttggagtg caaattcacc aaaaagtgcc gttattcttt ttcttcatta
    22861 aaacaagcgc gtaaacgtcg attggtcagc agccagatta acgccacgat atccgccact
    22921 accagcgcca gaccgatacc gctaacggat tcaccgttca gccacagcca cggttgccaa
    22981 caaagtaaaa ccacttgcgc caacagcaac agaaaataga gcacatgcca gatacgggga
    23041 aatgaagccc gccgaccact cagcaaaaat gccagcaccg ccggaatacc gggaatcaac
    23101 cccagccaga aattatcgtg atcgggataa aacagattta gcagcgcagt accctgctcg
    23161 cgcgacgctc cggcaatgac aaacagcacc caggttcgcg cctgaagcaa tagcacaagc
    23221 cagaagagca agggtaaacg caggcgaccg tgcgcatcat aatggacagg atgaaactca
    23281 gtactcttca tcttcaatca aacgcttacc cagactcagc acgtcggcgt gttcatatcc
    23341 caggcgttca tacattccga gcaccatgtc gttatcttcc ggcacgttga tctgaatttt
    23401 cggacagccg cgagcaatca gctttttctc cagccgatta agcaacgcat tagcaatccc
    23461 acgcccacga aactcgggat gcacgccaag ataataagca gacccgcgat gcccgtcata
    23521 accgcccatc accgttccga ccacttcacc gttcacctca gcgaccagaa acaaactgac
    23581 gtcatggttc atcttacgct cgatgtccat ttccggatcg ttccacggac tcagcaagtc
    23641 gcaacgctcc caaagggtga tgacctcttc gaaatcttcc tggcgaaata cgcgtatctc
    23701 catggtattc gttacctttt tgcgggttaa aaggctgatt atggcgtgaa cggtcgaatt
    23761 agccaatatc tgacgaaaat cggttgaaaa agtggcataa tggggagttg tcaactattg
    23821 aaatgaaaag taaaacaatt ctcaacagca aaccgtcgta acggattacg cgatacgata
    23881 taacatctgg aactttatta ttacaactca ggccgtatga gcacttttaa accactaaaa
    23941 acactcactt cgcgccgcca ggtgctgaaa gccggtttgg ctgccctgac gttgtcagga
    24001 atgtcgcaag ccatcgccaa agaagaaccg ttaaaaacca gtaacggaca cagcaagccg
    24061 aaagcaaaaa aaagcggcgg cagacgtgtc gttgttctcg atccaggcca cggcgggatt
    24121 gataccggag cgatcggacg caacggttcg aaagaaaaac atgtggtgct ggcaattgcc
    24181 aagaatgtcc gttccatatt gcgtaatcat ggcattgatg cgcgtttaac gcgttctggc
    24241 gatacgttta tcccactgta cgatcgcgtt gaaatcgccc ataaacatgg cgcagatctg
    24301 tttatgtcaa ttcatgccga cggctttacc aacccgaaag ctgccggtgc ttcggtattt
    24361 gccctctcta accgcggggc aagtagcgca atggcaaaat acctgtctga acgcgaaaac
    24421 cgcgccgatg aagttgccgg taaaaaagcg actgacaagg atcacctatt gcagcaagtg
    24481 ctgtttgatc tggtgcaaac cgatactatt aaaaacagcc tgacgctcgg ctcgcatatt
    24541 ctgaaaaaga ttaagccggt gcataaactg cacagccgca acaccgaaca agccgcgttt
    24601 gtggtgttga aatcgccgtc ggttccgtcg gtgctggtgg aaacctcgtt tatcaccaac
    24661 ccggaagaag agcggctgtt aggcacggcg gcgtttcgcc agaaaatcgc cacagcgatt
    24721 gccgaaggcg tgatcagtta tttccactgg tttgacaacc agaaagcaca ctcgagaaag
    24781 cggtaagtga tgaaacccga cgcacacctg gttaaacagt tcttgctcaa ccttcaggat
    24841 acaatttgtc aacaactgac cgccgtcgat ggcgcagagt ttgtcgaaga tagctggcaa
    24901 cgtgaagctg gcggcggtgg gcgtagtcgg gtgttgcgta atggtggtgt tttcgaacaa
    24961 gcgggcgtca acttttcaca tgttcacggt gaggcgatgc ctgcttccgc caccgctcat
    25021 cgtccggaac ttgccgggcg cagtttcgag gcgatgggcg tttcactggt agtgcatccg
    25081 cataatccgt atgttcccac cagccacgcg aatgtgcgct tttttattgc cgaaaaaccg
    25141 ggtgcagagc ccgtctggtg gtttggcggc ggcttcgatt taaccccttt ctatggtttt
    25201 gaagaagacg ccattcactg gcaccgcacc gcccgtgacc tgtgcctgcc atttggcgaa
    25261 gacgtttatc cgcgttacaa aaaatggtgc gacgaatact tctacctcaa acatcgcaac
    25321 gaacagcgcg gcattggcgg gctgttcttt gatgatctga acacgccaga tttcgaccac
    25381 tgttttgcct ttatgcaggc ggtaggcaaa ggctacaccg acgcttattt accaattgta
    25441 gagcgacgga aagcgatggc ctacggcgag cgcgagcgca attttcagct ctaccgtcgc
    25501 ggtcgttatg tcgagttcaa tctggtctgg gatcgcggca cgctgtttgg cctgcaaact
    25561 ggcgggcgca ccgagtcgat cctgatgtca atgccgccac tggtacgctg ggagtatgat
    25621 tatcagccaa aagacggcag cccggaagcg gctttaagtg agtttattaa ggtcagagat
    25681 tgggtgtaac tccctcaccc ccactctcgc atccgctgat gcagcgtcag tgacggcttc
    25741 tcggcaaaca gttgctgata atccgtggca aattgcccaa gatgccagaa tccccactgc
    25801 atggcggcgt cttttaccgt tgtgctttgt gaccacggac ttatgagttc gcggcgcacg
    25861 gcgttcaggc gaatacgttt cagccacgcg ttcggaccaa tgcccaaaat ggcgtgaaat
    25921 gcgttttgta gcgtgcggcg gctgacatgc agttgattac acaagtccag caccgtcacc
    25981 ggttcggaca tgttttccag cacatattca cgggcgcggg aaagcaatcg gcggtaactc
    26041 tgatggctga tgctttccgc cgtcatcatc ggctgcgctt cttccagcat cgcccccatc
    26101 gccatgagca aattatcccc cagcactttt cgcactgcag gctgatggag attttccggg
    26161 ttctcgcaaa acgtcgccag cgcctgttgg acaaagcccc acagagcggc tttatgctgc
    26221 tctttcactt ccagcgccga ctggctacgc agcatatgta atacccgatc cggattatgc
    26281 aaaaagttag cctgacgagt gatgacatct tcagaaagca ctacgccaag gatcgtgtaa
    26341 tcgtccggcg tacttaattc aaactcggtt ccacccgggc gcgtcgcaat ttctgcgctt
    26401 cccagacatt gcgaaccgat aaatcccagc tcaccgcgcg tcgccggaat gccaaaccag
    26461 aacgagttcg gccagaccag gcacgactga cgcagcgcca gaccagtgta ttcacgaaaa
    26521 acctgaatat catcgagtag aatttccgta aattcaccat gaaacttgcc cggatgcagc
    26581 tgatcgtaaa tctgctgcca ggcggtaatt gtcaaagcat gttcatagac gtccgttgtc
    26641 tgtcgttgat gaacattatc cacttcgacc ttcggcgtga gcttcaggtt ttcgggtaag
    26701 ggttcatgat aaagatggtg caaattggct gtacgggtct ttttcatgat gttaatgccg
    26761 ggtgttgtag gacacccggc acctccgaca ggttaatggg gcttgagacg gtaacgacta
    26821 ctgcgtttac gtaacgtccc ggcagaaaag agctgttcga gcgcatttct ggctttttcc
    26881 agcggccagc caaagtgggc agccacttct cctgccgtca ttccctgacg tactgatgtt
    26941 aacagcgcca acaactcgtc agcagattcc gcgacgataa ctggcgcgtc gggttccttt
    27001 acgggttgct taggctggcg gttaaagcca gtaaccagcc actgggtgtc atcgtctggc
    27061 cgaccgatct ccttgcggct gatgacacgc ccggtacgca tcgcggcagc acaacctgcg
    27121 tccagcgccg cacgacacgc cgccagatcg ccttccacca ccagcgttag gcgaccaggg
    27181 tcaagcactt cgtgactgag cagacgaacg ttagctgctt tgagcatggc atcagctgca
    27241 tctatcgcgg cgaccatgcc gtccacttcc agcaatccca gtgcattgat catcggcaac
    27301 ctccgttacg cacgctggat tggattacgc gcgatttcca gcactgcatc ggtaaaggcg
    27361 ttacaggctg ctttacacgc ggcctggcta ccggttaaaa atgctgccga gtagttggtt
    27421 tcagacggtg gcgggacata ggtcaccagc tgcacgtcgg cagatttcaa cgctgcatca
    27481 atgccgtagg tcgcttccag cggcggtgcc accagatacg ccatcggatc gccaagcgtg
    27541 atcccggcgg ttgatgagag ataagaaccg gtacgcgaaa ctacatgtgc caggaaggct
    27601 gtgttttctg cgtcattagc ccactggaaa gcagcgccat tttcaatatt cgcaaccatc
    27661 gcatccagac cagcacgcac ttccgccggg tttggtccac caagcataat cagcacctca
    27721 ccggcagtcg gtgacgggcc gtgtgcagcg cccgcataca gcgagcgacc atataccact
    27781 tcgaccatcg cctgcttggt cgcttcgtca gccgcaatgt aggtgacgtc atctgaatca
    27841 gcagaaatca gcccgaggct acgaatatgc ggcggcaatt tcagttcacg tgcaaattcg
    27901 gcgttaacgg aggcaatcac ccgcatggcg gttaccgacg gtcgaatcaa atctaaagct
    27961 ggcatgatgc ctccttatcg ggtcatgttg atgccggatg ctttctgctc cagcatacgt
    28021 ttcgccaaat ccacaatgac ggctgcggct tcaaccggcg gcgtgccgcc ctggtgaatg
    28081 ttagagatac aagtgcggtc agcctcaacg gtggtcgcca tacgcggcga gtaaacggcg
    28141 tagcaggaga ggctttctga ctgccccaac cccggacgtt cgcccaccag caggatcact
    28201 actttcgcgc cgaggatctc accgatctga tcttcaatct tcacgcgacc ataacgtaca
    28261 aagaatggcg taccgacttt cagcccggct tgcttcaggc ccgccatcag cggcggcagg
    28321 atctcttcgt agttcacggt gatcgcatca gttgacaggc catcagaaat aacgacctgc
    28381 acgtccggat tagcaacaca ctgtgctttc agcgcttcaa cagcttctgc acacaggcgg
    28441 cggcccatat ccgggcgagt caggtagaga tttttgtcgc tgatttcgga gcggacttcc
    28501 agcaagccct gcgctttcac ccactcttcc ggtacttctt tcagaacggt atctttcgaa
    28561 cgagagtgat cggccaggaa acgcagcaac gcctgggtgc gcggacgcgg accggcacga
    28621 ccggtacaaa cgcgggccac ggtgctgcga cgcagttctg ttaatacgtc tgcgtgatgc
    28681 gggttttcaa caccaatcca cgcttttgct tccgcagaac ctaaatccag cgcgcagctt
    28741 tccgaggtca ccggtgccgc acaggtggtg gtggcgcact ttgcttctga cggcgcgggg
    28801 gccgcttgtc ccattgacgc catcacgctg cgtacaattt cttcaatctg tttttgatcc
    28861 atgatgtgtt atctccgcgt catcagaaga acagtgacgg atcgcccgcc cgtttggtca
    28921 ggcgaccgtt tgccataatg cccatgcttt ccagccagcg ttcaaactcc ggtgacgggc
    28981 gcagattcag taactgacgc acagtggcag tgtcgtggaa tgcggtggtc tgatagttga
    29041 gcatgatgtc atcgcccagc ggcatcccca tgatgtagtt gcagcctgcg gtggcgagca
    29101 ggatcatcag gttttcgttg aggttctggt cagcgtcagc gtggttggtg tagcagcagt
    29161 cacagcccat agagatgccg ctcagtttgc ccataaagtg atcttctaag cctgcgcgga
    29221 taatctggcg gtcgttgtag agatactccg gcccaataaa gccgaccaca gtgttgacga
    29281 taaacggatc gtaatgacgc gccagcccat agttacgcgc ttccatcgtc acctggtctg
    29341 cgccgaagtt cgcgccagca gacagcgccg aaccttgtcc ggtttcgaag tagaggcagt
    29401 tttccccggc gatacggttg aactccgcgc ccactgcgcg tgcttcatcg agcatcgcca
    29461 gctctacgcc aaactctttc agcccttttt cgctgccgca gatactctgg aaaatcagcc
    29521 cgcctggtgc gccgcgacga atcgcttcga tttgggtagt gacgtgcgcc agtatgcagc
    29581 cctgagttgg gatgttgaat ttgtcgatca cgccatagat ggtatccaac acgcggctta
    29641 agttttccac gtcgtcagtc accgggttaa cgccgatcac cgcatcgccc accccgaagg
    29701 aaagcccttc gtagatttgc gcggcgatac tttgcacgtc gtcacgggta tcgttcggct
    29761 gcaagcgggc gctgaaggtg cccggaatac cgatggtggt attggccttt ttgatcaccg
    29821 gcattttctt cgcgccgtag atcaggtccg cgttggagca aatcttcgct accgccgcca
    29881 ccacttccga ggtcagccct ttgcgggtaa aggcaatgtc atccacgctg gtttcatcgc
    29941 tcagcacata ctcacgcagt tcgctgatgc tccagttttt aatctggttg taggccgttt
    30001 cgttaacgtc gtcctgaatc agccgcgtca cgcagtcatc ttcataggca atcaccggat
    30061 tattgcggat gtccgctaca gtcatttccg acaacacctg ctttgccgcc acgcgctcct
    30121 gtgagcttgc cgcagcaacg cccgccagca catcccccga acgcagttcg ttggctttag
    30181 ccagcacctc ttttacatcc ttaaactgat atacattgcc gaacaatgtg gtctttagtt
    30241 tcataagtcg ttccctcagg aaggaaatgc gagtgatttc accgtcaccg gcacaaccga
    30301 tccgccaaaa agaggcgtac caatgtcgat atagtccccc gcgcggacaa tcacttcgtc
    30361 aatgactgcc aacgggagtt gttgtagctg tgggcgcaac aacatgccca gagctttacc
    30421 aaagtcctgc ccggccacca ccagcagggg atgcggattc ggaaaacgcg cgacgaaatc
    30481 gaccagcgcg ttgatgaccg tcagtaccgc ggcgtaacgc acaggcagcg aggcgggaag
    30541 cgccagcacg tacgcgtcag ttttggggca aagatccagt tgaatcagcg cctgttgcca
    30601 ggcactgacc agatccgttt catcaatcgg gatcgccacc ggcaaattgc gcagcggcag
    30661 ttgtacgccc tccagccata ttgtgctgcc agagagcgaa agggtatgtg cacccgcgcc
    30721 aatcaccgtg gcgcgcacgg tttgcgccgg aaactgcacg ttcatctcac gcagacgcgg
    30781 atggtcatgc agcgccgttg ccagaagcgg gccgatatcg gcaaaacaga acgggtcggc
    30841 gggctggtgg cgataacatt cgcctacccc gccagaaagc gtaatgattt cgggcgtaac
    30901 acctgcgggc agcaaaccgg tttgcatcaa tgcctgcgcg agcggcgaga gcgttccgtc
    30961 aatcacttcg acaatcagtt ccgccatccg gcgggtaacc tgcgccaatt gcgcgccggt
    31021 cagcgaacgg gcgtcagtgc ctgggccgaa gcactcatcc acaatcatct gccccggttt
    31081 atgagcgtaa accacgcgcc cctggctgtc ggtttccagc aggcgaccgc cgacgttgag
    31141 gcaggcagtg ccgctgattt ttccggcatc gaacagggcg tagttcgcgg tgccaccgcc
    31201 gatgtcgata ttcagtaccc gacacagccg ttgttcagaa agggtttgcg ccccggcacc
    31261 gtgaccggcg atcacggatt cgagatgcgg cccggcgctg gcgacgacaa aatcgcccag
    31321 cgaccgagag agcgccatca ccgccgggcg agcgttgcgg gttttcgcgc tttcaccggt
    31381 gatgatgatg gcaccagaat caacgctttc cggcgcaatg cccgcagcct gatattgctc
    31441 gaggattaag gttttcagtt ccgcttcttt taaaccaccc tgtttatcga caggggtaaa
    31501 gaacaccggg ctttgccagc taatttcgcg tttgatgaat tcgtagcgcg gcacctgcga
    31561 caccgccgca cggttaacca gctccaggtg ggagaagatc acctgggtgg tggtggtgcc
    31621 gatatcgata ccgacgctca gtagctggcg agtgttcacg attgcgcctc cgcttcggtt
    31681 ttagccgcgc tcgcgtcttc ttttggcacc agcatcatcg ccacgccaat tgccgttacg
    31741 ccgccgatca atttgccgac aatcatcggg aagatcatgg cgttcatgtt ggcagcggcg
    31801 aagcctaaat ggtcgcccag cgcgaaagca gcggaaacgg cgaaggcaca gttgatgact
    31861 ttgccgcggg tatccatctg cttcatcatg ccaaacatcg gaatgttgtt ggcaagcgtt
    31921 gccaccatgc cggctgccgc gatattgttc atattcagca ccttaccgac gctcatcagc
    31981 ggtttttcaa accagcgagt cagcagcagt accattggat acgcccctaa cagaacgcag
    32041 gagatcgagc cgataacttc aatggcgcgc atcacttcac cgggtttatc gccaggggcc
    32101 ataaagatag gatcaaggcc cgggatcagt tcccagccaa ggaggaattt cactaccgca
    32161 gcggcaagac cgagggtgat caatgcaacg aggaatttgg cgaagatctg gaagccgttg
    32221 atcatttttt ccgggatgaa tttcagcccc agcgccacca gcaccgcaac gataagtacc
    32281 gggatcatgt tcatcaggat cagcgcaaag gtgaattcaa caggctggcc gttgatctgc
    32341 acaccggagt acatcgctac cagaccacca gcgatacagc caatcggaat ggtcactatg
    32401 cccgccagca cgccgagcgc cagataacga cggtcagaag gttcgataat gccgagcgcc
    32461 accggaatgg aaaacacaat cgttggcccc atcatcgacc cgagaattaa cccagagtat
    32521 agccacgctg ctacgtcacc gcccgccagc tctttggcga ggaagaagcc gcccatatcg
    32581 cacgccagca gtgttccggc gaacattgac gggtttgcgc cgagcatttc gtaaaccggg
    32641 ataatcaccg gtccgagaac gtgggccagt accggtgcca gtgcggtcat accgaccata
    32701 gccaggccca gagcgcccat tgccataaag ccttcttcga actgaccgcc ggatccttcg
    32761 atacttttac cgaacttacc gaggaaacga gcagaaccgc cgaactgcga caggatcctg
    32821 tctacggcag ctatcagcat aaagaacatc atgatgtaca tgatgatttc gttaattccc
    32881 atcgccttta ctccctgtta gttgttattt attggcggat gcggcgtgaa cgccttatcc
    32941 gccctacatg tgcaatcccg aaggccagat aagacgcggc aagcgtcgca tccggcaatt
    33001 gcaccgcgcc actggcggat gcggcgtgaa cgccttatcc gccctacatg tgcaatcccg
    33061 taggccggat aagacgcggc aagcgtcgca tccggcattt attgcgccgc tgcgtacagg
    33121 ccgacaatct gctcgaggct ggcggtacgc gggttactgc gcagacaaat atcttcctgc
    33181 gcggcctgcg cccatgcacc gtaatgcccg gttgttgcac ccacatcagc cagccgcttg
    33241 ttgatcccaa cttccgctat cagctcactt accgcattaa tagcgtcacg atcgtcggat
    33301 tttttagttc gcagcgcccg accaatctga ctaaaccgat cacgacaaac catccggtta
    33361 aattccatca ccgttggcag caacatggcg ttcgccagac cgtgcggaat atgcagcgcc
    33421 gcccctggct gatgcgccat cgcgtggcat aacccaagcc cggcgctgga aaacgccatt
    33481 cccgccatac atgaagccag caacatgctc tcgcgggcga caaggtcgtg accgtagccc
    33541 accgctttcg gcagcgattg accaatcatc gcaatggcgc caatcgccag gctgtcggta
    33601 aacggtgtag cgttcagcgc gctgtacgct tcaatggcat gggttaacgc atcaatgcca
    33661 gtcatcgccg tgacatgcga cggcacgcct tcggttagca cagcgtcgag aatcgcgaca
    33721 tccggcatca gcgaggcatg ggctaacacc tgtttgcgcc cgcttaccgc atcgataatc
    33781 accgtcacgt tggtggtttc agagccggtt ccggcggtcg ttggaatggc aatcaacggc
    33841 aggcgcggtt gcagaacgct ggtttctgac atttctgcca gcgtgctatc cgggttcgtc
    33901 accagcaacg ccacggcttt cgccgcatcc agcaccgagc cgccgccaaa cgcgatcacc
    33961 ccgtcacaac ctgattcacg caactgcgcc acggctgcac acacgtcggt gatgcacggt
    34021 tcgcccaccg gacatggcca gagcgtcatg gcgatacctt taacggccag gctacgcgtc
    34081 agcccagcgg tcatccctgc ctgatgcaaa aagctgtctg ccatcacgaa cagatgtttc
    34141 aacccacgcg tttgcgcttg ctgcccacaa ctgctcaccg cgcccggacc gcaaagcgtc
    34201 accggtggaa cgctaaatgt ttttacccgt tgcagattca gggtatcgaa cgcctgaaag
    34261 agcgcggtct gcaattcatt ttgcatatag cccctccgct ttctctcgcc cactactggc
    34321 gattgccagc ggggtcataa acaaactgtg ctgcggtaaa tgcacctgta acgccggaaa
    34381 ttgtttgcaa aacagcgccg ctacgcccgg ttgcatacag gaaccgcccg ccagccataa
    34441 atccgtaatg ccctgccctt caatatgacg ggccacaatg tccgccattt tttcgtacac
    34501 cggtttcacc gcgggccaaa tctcgtcacc gtgaccgcgt ttgtactgct ctgcctcttc
    34561 cagcgaaata cggcgatttc cggcgagagt cagagagatg tgatgtccgc cggtggcttc
    34621 atccgccgag tacgtcactt tgcccttttt cacgatggca atgccggtag tgccgccgcc
    34681 gatatccact acaccggcgt tgtccagttg cagcagatcc gcaactgcgg ttggctcatc
    34741 cagcacatgg ctcacttcca gtccggcaga ctccagcacg ttgatggaaa tacgcgggtc
    34801 cgtaccagga ggaaatgagg tcgccgcatg gctaaaacgg cgaccgaatt gctgctcaag
    34861 ggtgtcgaga tggcgacgaa caatggtgac cgcaccgaag aaatcccaga cgatgccgtc
    34921 gcggaccaca tcggcccagt cgaggcatac cgccaccggc tgaccgtcgc ggtcgacaac
    34981 catcgacacc acatcgcagg tgcccagatc cacccccagc cacagcggag attccgtcgc
    35041 ggcgggcgtc tggttacaca gcgttgccgc cgtttgcaga cgtggggtga gccattgttc
    35101 atcgtgcgcc atcggttact ccttaaacaa tgcgaaacgc atcgactaat acacagcgac
    35161 gcagacggac aaacgtccgc gcgctggtta ccccttcacc ggttggcgtg gtgatggtca
    35221 tcgtggtcca gccttccccg cccagcccca gcccggcaat acacggtccg ttcttaacga
    35281 aaatgctggt atcaatggcg ttcgccatct ggttcatgtt ttcgatattg cgcgagtgca
    35341 ttgccgccgt gtggtggcaa ccgccttcca gtttcaccgc cagcgcaatg gcatccgcca
    35401 cgttggcgac gcgcacgacg ggcagcaccg gcatcatcag ttcggtcacg gcaaacggat
    35461 gttctgcggt ggtttctaca aacagcagtc gcgtttgttc cggaacttta aggccgattg
    35521 ccgccgcgat tttcgccgca tcgcgaccaa cccagtcacg gctgacggtg ccttttccgc
    35581 gctcgtcgat atttttcagc aacaccgatt gcagttgctg cgcctgctct gcggtcagtt
    35641 tcaccgcatg ctggccttcc atcagacgca tcagttcatc ggctacgcta tcaacaacaa
    35701 tcagtacctt ttcgtcggca caaatgatgt tgttatcgaa agaagcgcct ttgacgatgg
    35761 actgagcagc acgggcgagg tcggcggttt catccaccac taccggcggg ttgccagcgc
    35821 ctgcggcaat cagacgttta ttggtgtgtt tacgcgccgc ttctactacc gcttcgccgc
    35881 cggtcaccac caaaaggccg atgcccggaa acttgaacaa gcgttgcgcg gtttcgatat
    35941 ccggatttgt tacggtaacc agtaagtttt ccggcccgcc tgcggcaaca atcgccaggt
    36001 tgagcagcgt aatcgcccgc tgggagactt ttttcgccgc cggatgcggg gcaaaaatga
    36061 cgctgttgcc cgcggcaatc aggctgatgg cgttgttaat tacggttgcc gccgggttag
    36121 tggaaggcgt cactgaagcc accacgcccc agggtgcgtt ttcaatcagg gtcgggccgt
    36181 tgtcgccagt cagcacttgc ggagagaggc actcaacgcc tggtgtgccg cgcgcctgag
    36241 cgacgttttt ggcaaattta tcttcaacgc gccccatgcc ggtttcactg acggcaagtt
    36301 ccgctaaatc tctggcgtgt ttttcgcctg cttcacgaat ggcagcaatg gctaactggc
    36361 gcattgccac gctttttaac ccttgctggg cgactttggc tgccgcaacg gcgtcatcca
    36421 gggacgcgaa aacgcccatc tcatgaacgg cggcgggcgg cgtgtcactg ctttgcattt
    36481 tcagcagtac cgctttcacc acctgttcaa tatcctgttg attcatgatg ttctgcctta
    36541 tttgtggaaa atgacctgac cgccagacac cacctcatcg acaatgccaa tcacgcacag
    36601 atcgaccggt gacgtttcgc ttttatgcgc ctggcgggcg gaactgccac tcaccagtaa
    36661 cacccactcc ccggttcccg cgccaatatt gtcgatggcg acggcgcatt gcccgtcagg
    36721 attaccttgt ggatcaatca tttccaccat cagcaatttg tcatgcgcca gtccgtgatg
    36781 gcgtacggta caaacaattt gtccagtgac gactgccagt ttcatacccg cctccgtggc
    36841 gtatttcagg taaaagctcc ccctaccctc cgcagaaggt aaaatgaaaa aggagagagc
    36901 gtgacgcccg aatcgacgtc acacagggtg attacaggtt gctgctatcg cctttcaggc
    36961 cgatcgggaa gacttcttcc aggtcgccgt gcggacgcgg gataacgtgt acagatacca
    37021 gctcgccgat acgctgtgcc gctgcggcac ctgcatcggt cgcggcttta caggctgcaa
    37081 catcaccacg aaccatggca gtacacaggc cgccgccaat ctgcttaaca ccaaccagct
    37141 tgacgcgcgc agctttaacc atcgcgtcag aagcctcaat cagtgcaacc aggccccggg
    37201 tttcgatcat tcctaatgct tccattgtgc tttcctcttt atcagggtcc agaacgggac
    37261 cgttcattca accagtgttt gtaaactgct ttcgcggttc acttctgtct gacgcggcac
    37321 agctgccacc agcgccagtt cgataatttc ctgcacgcta caaccacgag agagatcgtg
    37381 catcggcgcg gcaagtcctt gtatcaatgg cccgacggca cgatatccgc caagtcgttg
    37441 tgcgattttg tagccaatat ttccggcttc cagcgacgga aaaaccatca cattagcttt
    37501 gccctgtaac gggctggcag gcgctttttg cgccgccact tccggcacga aggcggcgtc
    37561 aaactgcaac tcgccatcga caaccagctt tggtgcgcgc tcacggacga tttctgtcgc
    37621 ctgctggacg ttggcaacac aggggtgacg ggcactaccg ttgctggaaa acgacagcat
    37681 cgccacgcgc ggttcttctc cggtgatggc gcgccaggtt tcggcgctgg caagcgcgat
    37741 atccgccagt tgtgccgccg tcggctgtgg caccacgctg caatcggcaa aacccaacgc
    37801 cggaccgctg tactgtggca gcatcaggaa aatggatgag agcgttttac agcctggctg
    37861 caagccgata atgcgtaatc cggcacgcag cacattcgcc gtggaagaga ggttgcccgc
    37921 gatacagaca tccgctttac cggcgctgac cattgcagcg gcgaacatca gcggatcggt
    37981 aagtttttcc agcgcatccg gcggcgtttt ttcgcccgcg cgggccagcc agcgatgagc
    38041 aaattcttcc cgcattgcga ggttgccatg cggatctatc acctgtagcc cgtccatcgc
    38101 cacgccgtga ctgagcgcaa actgacgaag ttcaaacgga ttggcgacca gaatgggcgt
    38161 tgccagacct tgttgatgta aatattgcgc agctttcagc acacgttgat ctaacgcatc
    38221 cggaaaaact actctggcgg gcgctcgcaa cgccagttca cgacaacgtt taataatcat
    38281 tgcgtttccc ccagtcgttg ttggatctgc ttcaccgtta gcggctgctt cgttacgctc
    38341 aggatcatca tcacgtagac cgtgctggaa aggcggttca gcgcctgcaa aatgtccggg
    38401 cgtaatactt caaagctgcg ggtgataaag acctgcgccg ctacggtttc tgtttcccgc
    38461 actttggtgc gtagcagatt gagcaacgcc gcatcgcgcc cgtggctggc ttccggcacc
    38521 agatgatcgt ggtcgagata acgcagcggc tggtgagaaa gccgatgcag atcttcgtca
    38581 gaaagcccga caatcgcttg ctcgcccagc ggttccccca gcgcatcggc acgcataatg
    38641 ttgccaagac gcgagcggat gtccgccagc cacggctgcc acggttctgc cagttcaatt
    38701 tgcagccaca ctgccagcgc aatggtgctg tcgagaacag cgcgaaaacc cagacgcgga
    38761 tcgcttttgg caaccatttt ttccgccgac aggtgagtca gcgtatccgg cttttttgcc
    38821 accggctggc ggcacagttc gcagcacgcc tgcggatgtt catcgctact ggtcagcccg
    38881 tgaacgggct gcggctgctg ctgttcatcg tcaacaaaca ggcggccctg ctcgtcaata
    38941 aacttgatgc gcagatggcg gctttccagt aactcccggg cagagggcgt caggcgactg
    39001 tccgcaggga gatggatctc tgcgccttcg ctgagcgtat ggttcgctct tagccatgct
    39061 tcggtgatga aatctttcat aaggattgcc agttagccgg ccaggcgaca tacaggaatt
    39121 tcacgctgga tgtcgtacca aattcgatgc tggagccttt cgggataaac atcacatcgc
    39181 ccgctttggc gatcatagtt tcgccttcgt ggcgaacatg cagctcccct tccagcacca
    39241 tgtcgatttc gtcgtagttc agggtccacg gaaagaaggc gttctcccac tgcatgaaac
    39301 cagcggccat actgctgccg tcgtctccgg tcacgagatc agtcaagcct acgcagtgcg
    39361 gttccgcgcc atcgaaacga ccaaacttga cgctgctgcc atcaataact ttgatgccgc
    39421 ctttgccggt caccgattta aagctcggct gcagtgcacc ctgttccagc gactgttttt
    39481 ccttcatcac tttttccatc agctgcgcga ccaggctttc ggtaaactga ccttccggca
    39541 gttgggcgat aatggtttca cggatgcgct ggctttcggt tttatcggct gatgcgctgg
    39601 cgggaacaga cgccgttacc gggatcgatt catcacattc ggtgatggta aaacccaaca
    39661 gatcagccac ttcacgagct tccggggtaa taatgctggc gcgcagaacc acggacattg
    39721 ccagttcgcc gcgcgcgtgt gcttcacgaa tatcattcgc tgtgatgagt tttttcacct
    39781 gcttcctcct gtttggtgag agatgccaga taatccacca gttgctgcac gctttgcggg
    39841 tcgtggctgt ttagctcgaa aatcggctct tcgaagcccg tctcaagcaa taactttcgc
    39901 gttgcggcga cgtcagcatc tggcatgtcc gttttactga tgacggcgat ttgccgtttg
    39961 ctgacgccaa tatccagcaa cccggcaggc aaccgacttt ccgggtcatt cgcgccgtgg
    40021 acatagatca gcatatccac atcctgtagc gtggtaatta aggcgtgata ccagcgggga
    40081 tggctaaaat attcacccgg cgtatcaata tcgccattat cattaaattc cacggcctgt
    40141 gtttttcttg cgagggtata attcccctgc aacgcattaa ataacgttgt ttttcctgcc
    40201 ccgacggtac ccacaaacgc aattcgtttc atgaatgcct cgtattaact tttggtcatt
    40261 tcacaaagcg tataatttaa taagcgtcct aaaccactga cggtttgtga taacgcttct
    40321 tctactgcgc ccaccgagcc ataaatcacc agcgcgccac taaaacgatc gagaaaaccg
    40381 atatgcacat cggcggcttt caacgctaaa tcaccggcga tcatcgccgt ttctccggga
    40441 gttagcgtca taatgccgat tgcgcccgca tccggaacgc cgattttctt cgccagttcc
    40501 tcgccgggat gcgcgatgag atgcgccagt gtgacctgtt taccgggcac aaattcctga
    40561 atgatgcgtt ctttatccat gagtcgccgc ctgtgaaaaa ctttcgtgta tcgtgacaat
    40621 ttttcgtcag agtaaataac aaaattcggc aagcgcgttt aaaaggtgag gtagatcact
    40681 aagaatggag agatatttgc tgtggtgcat a
//
LOCUS       DACADF010000023        39148 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.023, whole genome shotgun sequence.
ACCESSION   DACADF010000023 DACADF010000000
VERSION     DACADF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.023"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            98..1126
                     /locus_tag="ISB30_001198"
     CDS             98..1126
                     /locus_tag="ISB30_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP1988853.1"
                     /translation="MSVLYRWLEDRQIDLRARLKIGTLFTEAEFSGGLIEFLRKDMES
                     EDKVVSPHTFNNRLATIRQYFVWELDVYISSLPLSDSQYEVLQAARKRLMGWIDRAFI
                     NTPRAGSIPRKSLTNDEAEFLLQCLNPESPDSFAHFEPVKFRNFAAVSLMLNCGLRPG
                     ELLSLRVEDIQIGSISSVTVKRRSPDPLDKRKPRPSVKRNGRVLPLEGHHFVRMLDRY
                     IVEWREILEQKSSSETDYLILSDEGDPLSHSSLTQLFSRLRSEYSGLLPEILTPKSLR
                     HTFSSRMEQVLRAAGMEEDRRKQALAMLRGDSSLESQSVYIAQEVEEQARRALSDYQK
                     KLITGFNK"
     gene            1123..2721
                     /locus_tag="ISB30_001199"
     CDS             1123..2721
                     /locus_tag="ISB30_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1988854.1"
                     /translation="MNLLLHKAPQISDSALELPSQFFTREGVKVEITGDRWTFPLLYR
                     HSRLDFSRVENDTLKTSIKRCIIDQASRVSTHAGCQYWSDISAAIVSRQKKHSLLPDI
                     SADEFQERLIAVMEEAISQARNKHRLWALYRPIQWYIWCSENYSELGFSIAYAQELDS
                     LEIPGNPKGEAVRQEDPECGPLHRTLELQPIINALKQDQSKVYEHLQQKTAVALSIAF
                     GRNPANLTFLKETDLVDLTPGSSPPCYVLKMPRIKKRQLAPRDDMIDEYLEPEIAKHV
                     LELIDASRHRKLLIEVDGKAVEVTRPLFINPRKNAYAIDSKRWDEAYNMLSTGIANLV
                     KAFIKRHNIISPLTGELLHVTPRRLRYTLATGLAAEGISKRELARILDHTDTQHVNVY
                     FEMAGKIVEHLDKATAKGFSKYLNFFKGRLIDSDEDAVKGERDDKHLTFVDDQNPADQ
                     ADIGVCGESSVCHLNPPYSCYLCPKFQPYRHADHEHVLECLLAGREERLKKYENARLG
                     MQLDEVIAAVAQVAKLCEEGGHHV"
     gene            2714..4723
                     /locus_tag="ISB30_001200"
     CDS             2714..4723
                     /locus_tag="ISB30_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP1988855.1"
                     /translation="MSDGRSNRKAKVVPFVDRLERDRNENLNSLVSKAKLMKLEGFES
                     VVWEDPEWQVDAGRLVKLTGKNTKSASFGFLLSPKLGSAPLDGCWELVAKALFVLRFH
                     RKYQSAPNQRSFITAIGYVSFAAKQLGQELVGLTPEALDNASGLISKHYSETTAYNLH
                     KHVAEFAAHCDSNGLCRVLFQYKYAKMKRPASTGGINHKRLDDPEALDTKSDKLVDPA
                     VFRVIGELYLNVPKEHKYRFYILMLTLLACTGRRFSEISLLPNQQLSSDEEGIAYIEY
                     FPRKASRGDVFTPKRRLYLPSEVTPIVGDVLVELAEITFEARLTAEEIHKVGEPDLRF
                     LDGIPEDKKLYAADLTELGISHTVLGTTGWLRKQNLAWPDHSALTKQGIKPANPIFYT
                     YKSGLVEYCFRDFSGAYLSALHTDQFGKEYYLKDLLFIRSLGLSSGSYAHWLATSCSQ
                     SMFTTFLRYFPALAAEYASSSIEVDFTSHHFRHTLNTLLDEGGLSDLLQTEWFGRTNP
                     RDTKAYQHTSREKRALMLREDIKKGLVGGQLAEQIKVVPVEVQDAILKARIQAVHDVG
                     TGICIHNFSQTPCERHLQCSADCKDYVWAKDDKGRLDEQKRQYALTALARKKAEQQLD
                     STKPKKSADWLAHNDKKLKTLAAQLADNGVEHFDPEQYLHEVEHG"
     gene            4716..5192
                     /locus_tag="ISB30_001201"
     CDS             4716..5192
                     /locus_tag="ISB30_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HAP1988856.1"
                     /translation="MVKRFRRMSDSDINTIVADLDRWALGELGSKLTWAVLEERFGFS
                     RQSLQAKSEIKAAYDNAKRALSGGLVKTKEQATKEAEELQVEVDRLKAELDAYKRKEE
                     QWLRRWQQIAFHVRQKGIQMASVDKAPPEGAELPSNTETAQILRPFDKEVPPSGRV"
     gene            5253..6395
                     /locus_tag="ISB30_001202"
     CDS             5253..6395
                     /locus_tag="ISB30_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1988857.1"
                     /translation="MQKRLNTDKAQQAIDKAGLTQTAVADALGVTKEAVSQWLLHKSF
                     PRPNKLLQLGKLLNLSFDDLVIKEDPHAPKVAFRKMRGTKTKDYHIEKAQEIGRFLRH
                     LVPYLPFDTFEMPPVLKSPSCDYDYLRTVTAKVREDINLGPVECVDFTHLIRRFSELQ
                     AVVVPVMWGAKQRHENAVHIHLPDSGSTWVYLNLDTNTHDFKFWMAHELGHCLSPSLE
                     GDDAEDFADAFAASLLYPHELAEQAYEVLVKKPSTAAKINTLKQMAELQVISPNTILQ
                     QVNKYAKRLDRAEIIMGNSFFGAVTKFNQQFLNISEALFGDNELDEYGKPSARDYIEK
                     VEGAFETPFFDMLRQYLKEHDKGPGYVQTVLDMPLLDAQSIHAELT"
     gene            6395..6946
                     /locus_tag="ISB30_001203"
     CDS             6395..6946
                     /locus_tag="ISB30_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP1988858.1"
                     /translation="MPQSKILVDTNAYLRLAKTVRPLLFVPFGDNEYCLYILPELNEE
                     LAGRKLQSKFPWVDDEEFAENRKHFPQIGKKQKKSIQQTFEYVWDHVQTELPGPSKVD
                     TWYVAYALELGVPVVTDDQDMTDLAKAFDAQVMPTLELLKIMLDCGHTDMKTINGLVE
                     YWKYFADMPANFKPDYQRLFGDQ"
     gene            7044..7268
                     /locus_tag="ISB30_001204"
     CDS             7044..7268
                     /locus_tag="ISB30_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015818596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1988859.1"
                     /translation="MSDPILTIKDVADYLKVNERTIYRLAASSELPGFKVGNSWRFKQ
                     SELEQYIAAQHNRAGVSEAMKSPNSKSEDN"
     gene            7281..9422
                     /locus_tag="ISB30_001205"
     CDS             7281..9422
                     /locus_tag="ISB30_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAP1988860.1"
                     /translation="MAQLENIEAIEKRLWKSADTLRANSELASNEYFLPVMGLIFLRH
                     AYSRYLSLKDEIIANLPSRGGKTRELTKEDFSKKSAIYLKPEAQFDYLIALTDADNRA
                     EAIIHAMDSIETDYTNLRNQLPKQEYNNIPNDVLGMLLRTLNPEELKKATGDIFGRIY
                     EYFLTQFADQGAHDGGEFFTPVSLVQLIVNVLEPDHGKIFDPACGSGGMFVQSAHFME
                     RHAQDPHELTFYGHEKNRVTTRLAKMNLAVHGLEGNVEGGEAAITYYNDPHEGLFGTV
                     DYVMANPPFNVDEVDADKIKGDKRRLPFGLPGVNKNKKVSNANYLWIQYFYSYLNDSG
                     RAGFVMSSQASSAGRDEAKVREQLVKTGHVDIMVDIRGNFFYTRTVPCQLWFLNKNKP
                     AHLKDKVLMLDARNVYRKVTRKIYDFSPEQQQNLTAVVWLYRGEGERFVELVQQYIDK
                     SIFEARSCEQSEALACEPIPDFITQLGKFGQAFQPFMDKLEQGGVSVEPYVDFLNAKG
                     SVEGGWSAFQILTNDLQKWWNEYDYNDAASLLDFIDKDTCLKDLAEQSRNLIKEIDLA
                     YKLATRVIELAEANDAKDSELWDNTLLNGRSRSNLKKTADDARKLAVEQLKQVRYFYK
                     QAHWLLSRFPEGKLCDVEGLVKLVDIKAIEAADWSLTPGRYVGVVPEEVDEDFDFEET
                     LHDIHIELQGLNSEAIELAEKIARNFEELGV"
     gene            9419..10573
                     /locus_tag="ISB30_001206"
     CDS             9419..10573
                     /locus_tag="ISB30_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017050842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP1988861.1"
                     /translation="MSWQSEPLSALTLKIGSGSTPRGGDSVYISEGTTLIRSQNVYNN
                     EFSTDGLVYIDEKTAEKMKGVSVEKEDVLVNITGDSVARCCLVHDAVLPARVNQHVAI
                     LRTNPKKLLPHFLAFYMVSPFMQAKMLSWAGTGGTRKALTKGMLEGFEIPLPPVDVQD
                     KIVQKIKTYNDLIENNRRRIQLLEESARLLYQEWFVHLRFPGHKQVKITDGVPEGWSK
                     EPLENLLVLQRGFDLPVSKRIEGSVPIYASTGINGFHNVAKVKGPGVVTGRSGSLGTV
                     MYVAKDYWPLNTTLWVKEFKKASPIFATYLLRAMKLEGYNGGAAVPTLNRNDVHKVDV
                     LCPESKLMNEFEVQVENIFKQIDKLKEYNEKLAQARDLLLPKLMSGELTV"
     gene            10588..12720
                     /locus_tag="ISB30_001207"
     CDS             10588..12720
                     /locus_tag="ISB30_001207"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HAP1988862.1"
                     /translation="MNRAQAQQLLQTALANPAAEFRDGQWEAIDALVNHRQKLLVVQR
                     TGWGKSSVYFISTKIFRDRGMGPTIIVSPLLALMRNQIESAARLGIVAETMNSTNTDD
                     WRSVTQRILNNQIDCLLISPERLANDSFIETVLQPIADRIALMVIDEAHCISDWGHDF
                     RPDYRRIVNILRQLPANTPVLGTTATANNRVVADIQTQLGNIQIHRGPLIRESLSLQT
                     MVLPDQASRLAWLAQVIPTLPGTGIVYVLTQRDADLVAKWLNQQGISALAYYSGVTHP
                     NFVNYAGKEDTDLYRQFLETQLLENKIKVLVATTALGMGYDKPDLGFVLHYQAPSSIV
                     AYYQQVGRAGRGIENAIGVLMSGTEDNDIHAFFRRTAFPSQDDVQRLLAALEDADGLS
                     LEKLEKEANLPQKRIEHVLKYLRTESPSPVIAIGKTWFRAPVRYQMDLEHIRKITEIR
                     EREWSEINDYLNTSGCLMLALRNALDDPSQETCGKCENCIAEPVLTKVFDQEIGIRAT
                     QFLRHSEEIIKPRKQIAASNDEAARTFVEYEFPRNLGDLVAQEGRVLSRWGDAGWGRT
                     VADNKHAGRFSDELVEAMAEMIQQRWQPNPAPQWVCCVPSRNHPELVPDFARRLAARL
                     GLPFVDAVSKVKDNQPQKGQQNRFHQCRNLDGAFAVTQLYQGQPVLLVDDIVDSGWTL
                     TVVAALLQQAGSGVVYPIALASSSVKDS"
     gene            12717..14105
                     /locus_tag="ISB30_001208"
     CDS             12717..14105
                     /locus_tag="ISB30_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HAP1988863.1"
                     /translation="MNVSRFSSADGSLPANGSLSPTAQATLLLTSYFSKASSESAKPL
                     TNAEWGRFALWLKEKSTTPADLLVSDPKALLQGWHDARISAERIIELLSRGHSLALAM
                     EKWQRAGLWVVTRSDPEYPRRLKQQLKTDSPPVLFGCGNKALLSAGGLAVIGSRNASE
                     SDLAFTDQVGAKAAAEGIAIVSGGARGVDETAMLGAMKQGGVVIGVMADSLLKAATSS
                     KWRKGLMNGHAVLVSPFYPEAGFSAGNAMARNKYIYCLADSSLVIHSGKKGGTLNGAE
                     ENLKKGWVPLWVKHTTDKDAANADLVTKGGRWLEADNTQAFKIAELMCNEGGRVNQVK
                     EQMGDLFSMPAQPELFFEPESNPEPDSGPEEVIESEREIVVSESQTKIQAIPEQTAES
                     EAAPSGPVDFYQMFISELQHLAEKPVTVDALIESTQLHKSQLTEWLKRAVDDGVVKKL
                     NRPARFQVKNKK"
     gene            14118..17360
                     /locus_tag="ISB30_001209"
     CDS             14118..17360
                     /locus_tag="ISB30_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017050845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP1988864.1"
                     /translation="MAMTEETLVQEVTADYLLNQLQWDESVLGMYEKLGKEGDLGRLS
                     EQEVVLTRYLGEKLIELNPGLPDVAYQEALRVVCEMPASTNIVAVNKEKYSLHKNGVE
                     VSFHNEKGERVKKRLRLFDFDNYENNHFLLVREFWIKGDIYRRRADLVGFVNGIPLLF
                     MEVKNVHKDIRAAYEQNLADYKDTVPHLFYHNAFIILGNGIDAKIGSVSSKFEHFNDW
                     KRLNESEPGVVDMETLLKGTCSKANLMNIFENFTLFDQSSGRLVKIIARNHQYLGVSR
                     AIDAVINRGERMGKLGVFWHTQGSGKSYSIVFFAQMVHRKLGGNYTFVVLTDREDLDT
                     QIYKTFAGCGLVDNDKDPCRADSGKDLKELIGQQKAFVFTLIQKFNEKVDPKKPYSER
                     DDIIVITDEAHRTQYGTLSLNMRNALPNASFIGFTGTPLFKDDEITKKVFGDYISTYD
                     FQRAVEDKATVPLYYDARGEELVFTDEDGNEHTVADPKGINERIAEKLDELEIEDVDV
                     QQRLERELKRDYHIITATSRLDQIARDFVAHYANAWETGKAMFVCLDKITCVRMHKLI
                     DFYWQQEIKTKEKALLSATDEQDLAWRERQLAWMKETLMAVVVSEEQGEVAKFEKWGL
                     DIKPHRQLLKAGLELADGSRLDMEEAFKSSEHPFRVAVVCAMWLTGFDVPTLSTLYLD
                     KPLKAHTLMQAIARANRVAEGKNNGLIVDYCGILKNLRKALATFAGAGDEGHGGGEGE
                     NEPAKPNEELLESLNESISFVAEFLRKHDFELSRIITETGFAKNAAIAQAKEIINQND
                     ETRKRFEVMAREVFNKFKACINVPGVNGYRDMRDAINVLYKSLQADKEKADISDIMKD
                     LYEIVDGSIDTTHKVKEPKPDGDRIYDISKIDFERLRQEFSRSERKNTTVQSLKHAVE
                     KKLARLMMQNPLRTDYQEHYEKLVKEYNQEKDRVVIEKTFEALLKLNEELSHEEKRAI
                     RECLDEESLVLFDLLSKPNLQPKEIAKIKQVATTLLATLKAERLKVANWQQKESTRDA
                     VKQQIFDFLYDERTGLPVEQYEENEISDITERVFLHIYRAYPELPSPIYG"
     gene            17761..18881
                     /locus_tag="ISB30_001210"
     CDS             join(17761..18019,18019..18881)
                     /locus_tag="ISB30_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSen4 family transposase"
                     /protein_id="HAP1988865.1"
                     /translation="MKKRFSDEQIISILREAEAGVPAGELCRKHAISDATFYTWRKKY
                     GGMEVPEVKRLKSLEEENTRLKKLLAEAMLDKEALQVALGRKLLTTDQKREAVMLMCD
                     ATGLSQRRACRLTGLSLSTCRYEAHRPAADAHLSGRITELALERRRFGYRRIWQLLRR
                     EGLHVNHKRVYRLYHLSGLGVKRRRRRKGLATERLPLLRPAAPNLTWSMDFVMDALST
                     GRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTC
                     RALDQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINDW
                     RQDYNECRPHSTLNYQTPSEFAAGWRKGHSENEDSDVTN"
     gene            complement(19191..19472)
                     /locus_tag="ISB30_001211"
     CDS             complement(19191..19472)
                     /locus_tag="ISB30_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988866.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            19901..21487
                     /gene="pdeC"
                     /locus_tag="ISB30_001212"
     CDS             19901..21487
                     /gene="pdeC"
                     /locus_tag="ISB30_001212"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAP1988867.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIRLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLFLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(21490..21813)
                     /gene="soxS"
                     /locus_tag="ISB30_001213"
     CDS             complement(21490..21813)
                     /gene="soxS"
                     /locus_tag="ISB30_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAP1988868.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            21899..22363
                     /gene="soxR"
                     /locus_tag="ISB30_001214"
     CDS             21899..22363
                     /gene="soxR"
                     /locus_tag="ISB30_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAP1988869.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            22910..24259
                     /locus_tag="ISB30_001215"
     CDS             22910..24259
                     /locus_tag="ISB30_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP1988870.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            24410..26059
                     /locus_tag="ISB30_001216"
     CDS             24410..26059
                     /locus_tag="ISB30_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAP1988871.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(26095..26979)
                     /locus_tag="ISB30_001217"
     CDS             complement(26095..26979)
                     /locus_tag="ISB30_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1988872.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            27083..27493
                     /locus_tag="ISB30_001218"
     CDS             27083..27493
                     /locus_tag="ISB30_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP1988873.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            27486..28175
                     /locus_tag="ISB30_001219"
     CDS             27486..28175
                     /locus_tag="ISB30_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP1988874.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDVLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(28214..29863)
                     /gene="actP"
                     /locus_tag="ISB30_001220"
     CDS             complement(28214..29863)
                     /gene="actP"
                     /locus_tag="ISB30_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAP1988875.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(29860..30174)
                     /locus_tag="ISB30_001221"
     CDS             complement(29860..30174)
                     /locus_tag="ISB30_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAP1988876.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(30384..32342)
                     /gene="acs"
                     /locus_tag="ISB30_001222"
     CDS             complement(30384..32342)
                     /gene="acs"
                     /locus_tag="ISB30_001222"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAP1988877.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSIDAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLKGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            32734..34170
                     /gene="nrfA"
                     /locus_tag="ISB30_001223"
     CDS             32734..34170
                     /gene="nrfA"
                     /locus_tag="ISB30_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAP1988878.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            34215..34781
                     /gene="nrfB"
                     /locus_tag="ISB30_001224"
     CDS             34215..34781
                     /gene="nrfB"
                     /locus_tag="ISB30_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAP1988879.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            34778..35449
                     /gene="nrfC"
                     /locus_tag="ISB30_001225"
     CDS             34778..35449
                     /gene="nrfC"
                     /locus_tag="ISB30_001225"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAP1988880.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACRKVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            35446..36402
                     /gene="nrfD"
                     /locus_tag="ISB30_001226"
     CDS             35446..36402
                     /gene="nrfD"
                     /locus_tag="ISB30_001226"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAP1988881.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            36422..38140
                     /locus_tag="ISB30_001227"
     CDS             36422..38140
                     /locus_tag="ISB30_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAP1988882.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLIPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLTIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFGLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRARLPTLLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            38133..38516
                     /gene="nrfF"
                     /locus_tag="ISB30_001228"
     CDS             38133..38516
                     /gene="nrfF"
                     /locus_tag="ISB30_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAP1988883.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            38513..39109
                     /gene="nrfG"
                     /locus_tag="ISB30_001229"
     CDS             38513..39109
                     /gene="nrfG"
                     /locus_tag="ISB30_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAP1988884.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQARAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
BASE COUNT         9587 a         9363 c        10438 g         9760 t
ORIGIN      
        1 tttggatggt gatggtttgc cgattccctc tccaaatgaa tttctgttga gtcgaagaaa
       61 cttaagtaca aacacgctaa tcaggaatct tcgagagttg tctgttttgt atcgatggct
      121 ggaggacaga caaatcgatc tgagagcaag gctgaaaatt ggcacactat tcacagaggc
      181 tgaattcagt ggtggtctga tagagttcct aagaaaagat atggaatctg aagataaggt
      241 tgtctcgcct catacattca ataatcgatt agcaacgatc cgacagtact tcgtatggga
      301 gctcgatgtt tatatttcat ctttaccact aagtgattcc caatatgagg ttcttcaggc
      361 tgcaaggaag cgcctaatgg gatggattga tcgagccttt ataaatactc caagggctgg
      421 aagtattccg aggaagtctc taaccaatga cgaagccgag ttcttactcc agtgtctgaa
      481 tcctgaaagt ccagattcgt ttgcgcattt cgagcctgtt aaatttagaa acttcgcggc
      541 ggtgtctctg atgcttaatt gcggacttcg ccctggggaa ttgcttagtt tgcgtgtaga
      601 ggatattcag attggctcga tctcgtcggt gactgttaaa agaaggtccc cggacccgct
      661 cgacaagaga aaaccaagac ccagtgtcaa gcgcaatggc agggtattgc ctttagaagg
      721 acatcacttt gtgcgaatgc tggatcgcta catagttgag tggagggaga ttttggagca
      781 gaaatcgagc agtgaaaccg actatttgat cttaagtgat gaaggcgacc ctctgtctca
      841 ttcgtctctt acgcaacttt tttctcgtct acggagtgaa tattctggtt tgcttccgga
      901 gattcttaca cctaaatctc tgaggcatac cttttctagc cgtatggaac aggtacttag
      961 ggcggcaggc atggaagagg atagaaggaa gcaagccttg gctatgttgc gaggagactc
     1021 aagtttggag tcccaatctg tgtatatcgc tcaagaggtt gaagagcagg ctcgtcgggc
     1081 actttctgac tatcaaaaga aattgattac agggttcaat aaatgaattt gctcttgcat
     1141 aaagcgcccc agatttctga ctctgcgcta gagttaccct ctcaattttt tactcgagag
     1201 ggtgtaaaag ttgagattac tggtgataga tggacatttc ctcttctgta tcggcatagc
     1261 aggcttgatt tttctcgagt tgaaaacgac acgcttaaaa cttcgattaa gcgctgtatt
     1321 attgatcagg catcaagggt gtcaactcat gcagggtgcc agtactggag tgatatatcc
     1381 gctgcaatag tctctcgcca aaaaaagcac tccttgttgc ccgacatttc cgctgatgaa
     1441 tttcaagaac gtctgattgc tgtaatggaa gaggcaatca gtcaggcgcg aaataagcac
     1501 aggctgtggg cgttgtaccg gccaattcag tggtacatct ggtgctctga aaactattcg
     1561 gagctcgggt tctcgatagc ctatgcacaa gagctcgatt ctctagaaat tccaggcaac
     1621 cctaagggtg aggcggttcg tcaggaagat ccagaatgtg ggccgttgca taggacgctg
     1681 gagcttcagc ctataatcaa tgcgcttaag caggatcaaa gtaaagtcta cgagcatttg
     1741 cagcaaaaaa cagctgtggc tctatctata gcttttggcc gaaatcccgc gaatttaaca
     1801 tttttaaaag aaactgattt agttgacctg acaccgggtt catcgccacc gtgttatgtc
     1861 ttgaaaatgc caagaataaa gaaaaggcag ttggctccaa gggatgacat gatcgatgag
     1921 tatttagagc ctgagatcgc aaagcacgtt ttggagctta tagatgcgtc caggcatagg
     1981 aagcttctaa ttgaggtaga tggaaaggca gtagaagtca ccagacctct atttataaac
     2041 cccagaaaaa atgcttatgc gattgattca aagcgttggg atgaggctta taacatgctg
     2101 tccactggga tagcgaacct tgtgaaagct tttataaaac gtcacaacat tatctcgccg
     2161 cttactggcg aattattgca tgtcacgcca aggcggctgc gatatacctt ggcaactggg
     2221 ctggctgctg agggaatcag taagcgtgag ctggccagaa tactggacca tacagatacc
     2281 cagcacgtga atgtctattt tgagatggct ggaaaaatag ttgaacacct agacaaagca
     2341 accgcgaaag gtttctcgaa gtacctcaat tttttcaaag gcaggctaat cgatagtgat
     2401 gaagatgctg tcaaaggtga gcgagatgac aagcatctaa cgtttgtaga tgatcagaat
     2461 cctgctgatc aagctgatat tggcgtgtgt ggtgaatcta gcgtctgcca ccttaatccc
     2521 ccctactcat gttatttgtg ccccaagttt cagccatatc gtcatgctga tcatgagcat
     2581 gtcttggagt gcctgctggc cggtcgcgaa gagcgtttga agaagtacga gaatgcgcga
     2641 ttgggcatgc agctcgatga ggtaatcgca gcggtcgcac aagtagccaa actctgtgag
     2701 gaaggggggc atcatgtctg atggtcgctc taatcgcaag gccaaggtcg tccctttcgt
     2761 tgatcgtctt gagcgtgaca ggaatgaaaa tctcaactct ttagtcagca aagcaaagct
     2821 aatgaagctg gaagggtttg agtcggttgt ttgggaggac cccgagtggc aagtcgatgc
     2881 aggtcgccta gtcaagttaa ctggtaaaaa tacgaaatca gcctcttttg gttttttgct
     2941 atctccgaag ctgggttcag cgccactaga cggttgctgg gagctggttg ccaaagcatt
     3001 gtttgttctc aggttccaca gaaaatatca atcagcaccg aatcagcgca gtttcattac
     3061 cgctattgga tatgtctcat ttgcagctaa acagttaggg caggaattag ttgggcttac
     3121 gccagaggct ctggataatg cgtctggttt aatttcaaaa cactacagtg aaaccactgc
     3181 ctacaacctt cacaagcatg ttgcagagtt cgcagcgcat tgtgattcga atggtctatg
     3241 tcgggtttta tttcagtaca aatacgccaa aatgaagcgt cccgcgagca cgggagggat
     3301 aaatcacaaa aggttggatg atccagaggc tctggatacg aagtcggaca aacttgtcga
     3361 tcccgctgtg tttcgagtca ttggcgaact ctacttaaac gttcccaaag agcataagta
     3421 tcgcttctat attctgatgt tgacgctact tgcttgcaca ggaaggcgat tcagtgagat
     3481 ttcactatta ccaaatcagc aattgtcttc tgatgaagag gggattgcct atatcgaata
     3541 ctttccgcgg aaggcaagtc gtggtgacgt tttcacacct aagcgtagac tttacttgcc
     3601 gtcagaagtg acccccatcg ttggtgatgt gctcgttgag ttagcggaaa ttacatttga
     3661 agcgcgttta acagcggaag aaattcacaa agttggtgag cctgatctaa ggtttctgga
     3721 tggcatacct gaagataaaa agctctacgc ggctgattta actgaattgg ggatcagtca
     3781 tactgttttg ggcacaactg gctggctcag gaagcaaaac ctcgcgtggc cagatcatag
     3841 cgcattaacc aagcagggaa ttaagcctgc aaatcccatt ttttacactt acaaaagtgg
     3901 attggttgaa tactgtttcc gtgatttctc gggagcatat ttatcggctc ttcatacgga
     3961 ccaatttggt aaagaatact acctaaaaga tcttttattc attcgttctc ttggactttc
     4021 cagcggaagc tacgctcatt ggctggccac aagttgtagc cagtccatgt ttactacatt
     4081 tctccgctat ttccctgcgc tcgccgccga atacgcctcc agcagtatcg aggtggactt
     4141 caccagtcat cacttccgcc atacgctcaa tactctgctt gatgagggcg ggttaagtga
     4201 cctgctgcaa actgaatggt ttggtcgaac gaacccaaga gacaccaaag cctatcagca
     4261 tacatcccgt gaaaaacgag cactgatgct ccgtgaagac atcaaaaaag gtttggttgg
     4321 aggtcagtta gctgagcaaa tcaaagtggt tccggtagaa gtacaggatg ccattctgaa
     4381 agcacgaatt caggccgtac atgatgtcgg tacaggtatc tgcatccaca atttctctca
     4441 aaccccctgc gagcgccatt tgcagtgttc ggcggactgt aaagactacg tgtgggctaa
     4501 ggatgataag ggtcgtctgg acgagcaaaa acgccaatat gcgcttactg ctctagcccg
     4561 aaaaaaagct gaacagcagt tggatagcac taagccaaaa aagagtgctg actggctggc
     4621 acacaacgat aaaaaactga aaacactggc tgcccaactg gccgacaacg gggtggagca
     4681 ctttgatccc gaacaatatt tacacgaggt agagcatggt taagcgcttc agacgcatgt
     4741 ccgacagtga catcaataca atcgttgccg atttggatcg ctgggcgttg ggtgagcttg
     4801 gatcaaaact cacctgggct gtgctagaag agcgctttgg ctttagtcgc cagtcgctcc
     4861 aagctaaatc tgagatcaag gccgcctatg acaacgccaa gcgagcattg tcgggcggat
     4921 tggtgaaaac caaagagcag gccactaaag aggccgaaga gcttcaggta gaggtagatc
     4981 gcctcaaagc ggagctagat gcctacaagc gaaaagaaga gcaatggctg cgccgctggc
     5041 agcaaatcgc ctttcatgtg cggcagaaag ggatacagat ggctagcgtt gataaggccc
     5101 caccagaagg ggctgagctg ccctcaaaca ctgaaaccgc gcagatactc cggccgtttg
     5161 ataaggaagt tccaccttca gggagggttt agtaggcact agtctggaga ttttaaactt
     5221 gacaaaactt gactgcggca tatacttcgc gcatgcaaaa aagactaaat acagataaag
     5281 cacagcaggc gatcgataag gcagggttaa cccaaactgc cgtcgcggat gctctaggcg
     5341 taaccaaaga agccgtttcc cagtggcttt tgcacaagtc ttttccccga cctaacaagc
     5401 tacttcagtt aggcaagttg ttaaatctct cttttgatga tttggtcatc aaggaagatc
     5461 cgcacgcccc gaaagtggca ttccgcaaaa tgcgcggtac caaaaccaag gattaccata
     5521 tcgaaaaagc gcaagagatt ggtcgctttt tgcgtcacct cgtgccctat ttgccgtttg
     5581 acacctttga aatgccgccg gtactcaagt cgccgagctg tgattatgac tatcttcgaa
     5641 cggtaacagc gaaagtcaga gaagatatta atcttggccc ggtggagtgt gtggatttca
     5701 cgcatttgat tcgccgcttc agcgaactac aggccgttgt ggtcccagtt atgtggggag
     5761 caaagcagcg tcatgaaaat gcagtacaca tacaccttcc tgactcgggc agcacatggg
     5821 tatacctgaa tctggatacc aatacccatg acttcaagtt ctggatggca catgagttgg
     5881 ggcactgtct ttcgccaagc cttgagggtg atgacgcgga agacttcgcc gatgcttttg
     5941 ccgcgagctt gctttaccct catgaattag cggagcaggc ctacgaagtg ttggttaaaa
     6001 agccgtcgac tgctgcaaag atcaatacgt taaagcagat ggcagagctg caggtaattt
     6061 ccccaaatac tatccttcag caagttaaca agtacgcaaa gcggcttgat agagctgaaa
     6121 ttataatggg taattccttc tttggtgcgg taaccaaatt taaccagcaa ttcctaaata
     6181 tcagcgaagc cctgttcggt gataatgagc tggacgaata cggcaagcca agcgcgcgcg
     6241 actacatcga aaaagtagaa ggcgctttcg aaactccttt cttcgatatg cttcgacaat
     6301 atctgaaaga gcacgataaa gggccgggtt atgttcaaac tgtgctggat atgccactcc
     6361 tcgatgcaca gagtatccac gcagagttga cctgatgccg caatcgaaaa tcctcgttga
     6421 taccaacgcc tacctaaggt tggccaaaac cgtacgcccc ttactgtttg tgccctttgg
     6481 tgacaacgag tattgcctgt acattctgcc tgaactcaat gaagagctgg cgggtaggaa
     6541 gctgcaaagt aagttccctt gggtagatga cgaggaattc gccgagaatc gcaaacactt
     6601 cccccaaatc ggcaaaaaac aaaagaagtc cattcagcaa accttcgaat acgtgtggga
     6661 tcatgtgcaa accgagttgc cgggtccgtc caaggtggat acttggtatg tcgcctatgc
     6721 attggagttg ggtgtaccgg tggtgacgga cgatcaggat atgactgatt tggccaaagc
     6781 cttcgatgcg caggtcatgc caaccttaga gctgctcaaa atcatgctgg actgtggcca
     6841 tacagacatg aagaccatca atggcctggt tgagtactgg aagtactttg cagacatgcc
     6901 tgccaacttc aagcccgact accagcgcct gtttggtgat caataagcct gaaagctttt
     6961 cttacttgat ctccctccca gatcctatat attgacagtc ttgtactttt tcgacacctc
     7021 cgacatagct gagatacctc gtcatgagtg accccatatt gaccatcaaa gacgtggccg
     7081 attacctcaa ggtgaatgaa cggaccatat accggttggc tgcaagcagc gaattaccgg
     7141 gctttaaagt gggtaactcc tggcgattca aacagagcga gctggagcaa tacatcgccg
     7201 cccagcacaa ccgcgccggt gtcagcgaag caatgaaatc accaaacagt aaatcagaag
     7261 ataactagga atccggcccc atggcacaac tggaaaacat cgaagcaatt gaaaaacgcc
     7321 tgtggaaatc ggctgatacc ctacgcgcca actccgagct ggccagtaac gaatactttt
     7381 tgccggtgat ggggctgatc ttcctgcgac atgcttacag ccgctactta tcgctaaaag
     7441 acgagataat cgcaaacctg cccagccgtg gcggtaaaac ccgcgagctt acaaaagaag
     7501 atttctctaa aaagagcgcc atctatctca agccagaagc acagttcgac tacctcattg
     7561 ctctgacgga tgctgataat cgcgccgaag ccattattca tgccatggac tccatcgaga
     7621 ccgattacac caacctccgt aaccagctgc cgaagcagga atacaacaac attcccaatg
     7681 atgtattagg tatgttgctg cgtacgctca acccagaaga gcttaaaaaa gccacgggtg
     7741 atatctttgg ccgtatctat gaatacttcc tcacccagtt tgccgatcag ggtgcgcacg
     7801 atggtggtga atttttcacg ccggtatcgt tagtgcagtt gattgtgaat gtgttggagc
     7861 cagatcacgg caaaattttt gacccggctt gcggttccgg gggcatgttc gtacaaagtg
     7921 cccactttat ggagcggcac gcgcaagacc cgcatgaact caccttttac ggccacgaaa
     7981 aaaatcgcgt gaccacacgc ctggccaaaa tgaacctggc ggtacacggc cttgaaggca
     8041 acgtggaagg tggtgaagcc gccattacct actacaacga tccccacgaa ggcctgttcg
     8101 gcactgtcga ctatgtgatg gccaacccgc cctttaacgt ggatgaagtc gacgccgata
     8161 aaatcaaagg cgataagcgc cgcttgccgt ttggcttgcc gggcgtaaat aaaaacaaaa
     8221 aagtcagtaa cgccaactac ctgtggattc agtactttta tagctatctc aacgattcgg
     8281 gccgagcagg ttttgtcatg tcatcccagg cttccagtgc tgggcgggac gaagctaagg
     8341 tgcgcgagca gttggtcaaa accggtcatg tggacatcat ggtcgatatt cgcggtaact
     8401 ttttctatac ccgcaccgtg ccctgccagt tgtggtttct gaataaaaac aagcccgcgc
     8461 acctgaaaga caaggtgtta atgctggatg cacgcaacgt ctaccgcaaa gttacccgca
     8521 agatttacga cttcagcccc gagcagcaac agaacctgac tgccgtggtc tggctatacc
     8581 gtggcgaagg agagcgtttt gttgagctgg tgcagcagta tatcgacaag tcaattttcg
     8641 aagctcgctc ctgtgaacaa tctgaagcgc tagcctgcga gccaatacca gactttatca
     8701 ctcagttggg aaaattcggc caagcctttc aaccctttat ggacaaactg gagcagggcg
     8761 gcgtgagtgt tgagccttat gtcgattttc tgaatgccaa aggctcggtc gaaggcggtt
     8821 ggtccgcgtt tcaaatcctg accaacgacc tgcagaaatg gtggaacgaa tacgactata
     8881 acgatgctgc cagcctgctg gacttcatcg ataaagatac ctgcctaaaa gacctggccg
     8941 aacaaagccg caacctgatt aaagaaatcg acctggctta taagctggca acccgcgtga
     9001 ttgaactggc cgaagccaat gatgcaaaag acagcgaact ctgggataac acgctgttaa
     9061 atggccgcag tcgcagcaac ttaaagaaaa ccgccgatga cgcccgcaag ctggccgtag
     9121 agcaactcaa gcaggtacgc tacttctaca agcaagccca ctggctgtta agccgcttcc
     9181 cggaaggcaa gctctgtgat gtggaaggcc tggtcaaact ggtagatatc aaagcgatcg
     9241 aagccgctga ctggagcctg acgccgggcc gctatgtggg tgtggtgccg gaagaggtgg
     9301 atgaagactt cgattttgaa gaaaccctgc atgatattca tattgaactg caaggtttga
     9361 attctgaggc catagagcta gccgaaaaaa ttgcccgtaa ctttgaggag ttgggggtat
     9421 gagctggcaa agcgaacctc tgtcagcgtt aaccctcaaa attggtagcg gctcaactcc
     9481 aagaggtggt gattctgtct atatatctga aggtacaacg ttaatccgta gccaaaatgt
     9541 atataacaat gaattttcaa cggatggctt agtttatatc gatgaaaaaa cagcagaaaa
     9601 aatgaaaggc gttagtgttg agaaggagga tgttcttgtc aacattaccg gcgattctgt
     9661 tgcacgatgc tgtttggtcc atgatgcagt actcccagca agggtaaatc agcacgttgc
     9721 gattttaaga acaaatccaa agaagcttct gccacatttt cttgctttct acatggtatc
     9781 acccttcatg caggccaaga tgctttcttg ggctggtact ggtggaacca gaaaagcact
     9841 gacaaaaggc atgctagagg gatttgaaat accactcccc cctgttgatg ttcaagataa
     9901 aatcgtccaa aagattaaga cttataacga cctcatcgaa aacaaccgcc gccgtattca
     9961 gttgctggaa gaatccgctc gcctgctgta tcaggaatgg tttgttcact tgcgcttccc
    10021 cggacataag caagttaaga ttactgatgg tgtgccggaa gggtggtcaa aggaaccttt
    10081 agagaactta ctggttcttc agcgtgggtt cgacttgcct gtttccaaac gtatagaagg
    10141 aagcgttccc atctatgcat ccacaggtat taatggattc cataatgtgg cgaaggtaaa
    10201 aggccctggt gtcgtcacgg gaagatctgg ttcactggga acggtaatgt atgttgctaa
    10261 agactattgg ccactcaata cgacattgtg ggtgaaggaa ttcaaaaaag cgtcacccat
    10321 attcgcaaca tatttgctaa gagcaatgaa gcttgaaggc tataacggcg gtgcggctgt
    10381 tcccacatta aatcgaaatg acgttcataa ggtcgatgtt ctgtgccctg aatcgaaatt
    10441 aatgaatgaa ttcgaagttc aggtagaaaa cattttcaaa cagatagaca aactaaaaga
    10501 atataacgag aagctagccc aagcacgtga cctgctacta cccaaactta tgagcggcga
    10561 gctaaccgtg taaatcaagg agtgatcatg aaccgtgccc aagcccaaca attattacaa
    10621 accgctctag ctaatcccgc cgccgaattc cgtgatggac agtgggaggc cattgatgca
    10681 ctggtgaatc atcggcagaa actgttggtg gtacagcgca ccggttgggg taaaagctcg
    10741 gtttacttta tcagtaccaa aattttccgc gatcgtggta tggggccaac cattatcgta
    10801 tcgcctttgc tggccttgat gcgtaaccaa attgaatctg ctgctcgact tggcatagtc
    10861 gccgagacga tgaattcaac caatactgat gattggcgtt cggtaactca gcggattctg
    10921 aataatcaaa tcgactgctt gttgatttcc cctgagcgct tggctaatga cagctttatt
    10981 gaaactgtgt tgcagcccat tgccgaccgc atagccctga tggtgattga tgaagcgcac
    11041 tgtatttccg attggggtca tgacttccgc cccgactacc gtcgtattgt gaatattctt
    11101 cgccagttgc ctgccaatac gccggtgctg ggcactacag ccacggcgaa taaccgcgtg
    11161 gttgcggata ttcaaactca gcttggaaat attcaaattc accgtggtcc gttgattcgg
    11221 gaaagtctct ccttgcagac catggtgtta ccggatcagg catctcgttt ggcttggttg
    11281 gcgcaggtga tacccacgtt gccgggtaca ggtattgtct acgtactgac tcagcgtgat
    11341 gccgatttgg ttgcaaaatg gttgaatcag caggggatat ctgcactggc atattacagt
    11401 ggcgtaacgc atcctaattt tgttaattat gcagggaaag aagatacaga tctctaccgc
    11461 caatttctgg aaactcagtt actagaaaac aaaatcaaag ttcttgttgc aactactgca
    11521 ttagggatgg ggtatgacaa acctgaccta ggctttgtgc ttcattacca agccccaagt
    11581 tcgatagttg cttactatca acaagtaggt cgcgccggtc gtggaatcga aaatgcgatt
    11641 ggcgtactaa tgtcaggtac tgaggataac gacattcatg ctttctttcg ccgaacagcc
    11701 tttccaagtc aagatgatgt tcagcgtcta cttgcagcat tagaagatgc agacggattg
    11761 tcacttgaga agttagagaa agaagcaaac ctgccgcaaa agcgaataga gcatgttttg
    11821 aaatatttgc gtactgagag tccatcgcct gtcattgcga ttggaaagac ttggtttaga
    11881 gctcctgttc gctaccaaat ggatctagag catattcgta aaattacaga gattcgtgag
    11941 cgcgaatgga gcgagattaa tgattatctc aatacttcag gttgtttaat gttggcttta
    12001 cgtaatgcgc ttgatgaccc ttcacaggaa acttgtggca aatgtgagaa ctgtattgcg
    12061 gagccagtgc ttaccaaagt tttcgatcaa gaaattggaa taagagcaac tcagttttta
    12121 agacactcag aagaaattat taagccccgt aagcaaatag cagcttcgaa tgatgaggca
    12181 gcgagaacat ttgttgaata tgaatttccg cgtaatctcg gtgacttagt cgctcaagag
    12241 ggccgtgttt tatcccgctg gggcgatgcc ggttgggggc ggacggttgc cgacaacaaa
    12301 catgccgggc gtttcagtga tgagttggtt gaagccatgg cagaaatgat tcagcagcgt
    12361 tggcagccta accccgcacc tcaatgggta tgttgcgtgc cgtcgcgtaa tcatcctgaa
    12421 ctggtaccgg attttgctcg ccggctggct gcacgcttgg ggctgccgtt tgtggatgca
    12481 gttagcaaag tcaaagacaa tcagccgcaa aaaggccagc agaatcgctt ccaccaatgt
    12541 cgcaatctgg atggtgcatt tgctgtgact cagctttatc aggggcaacc ggttttgtta
    12601 gtggatgaca tagtggactc cggctggacg ctaacggtcg tcgcggcact cctccagcaa
    12661 gcaggcagcg gcgtggttta ccccatcgcg ctggcttcat cttcggtgaa agactcatga
    12721 atgtatcccg cttttcatca gctgatggct ctttaccagc taacggctct ttgtcaccca
    12781 cggcgcaagc gaccttgctg ttgaccagct atttctcaaa agccagtagc gaaagcgcaa
    12841 agccgctgac caatgccgaa tggggtcgct ttgccttgtg gttaaaagaa aagtccacca
    12901 caccagctga tctgttggtt tccgacccga aagctttgct gcaaggctgg cacgatgcgc
    12961 gtatcagtgc ggagcgcatc attgaattgc tgagccgtgg tcatagcctc gcactggcca
    13021 tggaaaaatg gcaacgggca ggcctgtggg tggtcacccg ttccgacccg gaatatccaa
    13081 ggcgtttaaa gcagcagcta aaaacagatt ccccgccagt gctgtttggt tgtggtaaca
    13141 aagccttgtt gagtgccggt ggcttggcgg ttattggctc gcgcaatgcc agtgagtctg
    13201 atttggcttt taccgaccaa gtgggggcta aagcagcggc agagggtatt gccattgtct
    13261 ctggtggtgc tcggggtgtc gatgaaacgg caatgctggg ggctatgaaa caaggcggtg
    13321 tggtgatcgg cgtgatggcc gatagcctgc taaaagcagc gaccagcagt aagtggcgta
    13381 aaggtctgat gaatggccat gcagtgctgg tatcgccttt ctaccccgag gcggggttta
    13441 gtgcgggtaa tgccatggct cgtaataaat atatctactg tctggcagac agctctctgg
    13501 tgattcactc cggtaaaaaa ggtggcacgc tcaacggagc agaagaaaat ctgaaaaagg
    13561 gatgggtgcc gctgtgggtt aaacacacga cggacaagga tgccgccaat gccgatttgg
    13621 tcaccaaagg tggccgttgg ctggaggctg ataatactca agccttcaag attgcggaat
    13681 taatgtgcaa tgaaggtggg cgtgtgaatc aggtgaagga acaaatgggc gatctctttt
    13741 ccatgcctgc acagcccgaa ttgttttttg agcctgaatc taatcctgag ccagattctg
    13801 ggcctgaaga agttatagag tcagagcgcg agatagtcgt tagcgaaagc cagacaaaga
    13861 ttcaggctat tccagagcag actgccgaaa gcgaagctgc cccatcaggc cctgtcgatt
    13921 tctatcagat gtttatctcc gaacttcaac acttggctga aaagccagta accgttgatg
    13981 cgctgattga aagcacacag cttcataagt ctcagttaac tgagtggctg aaacgtgctg
    14041 ttgatgacgg ggtggtgaaa aagctcaatc gccctgcacg ttttcaagtc aagaacaaga
    14101 aatagaagga tttgctcatg gccatgacag aagaaaccct ggttcaggaa gtcactgcag
    14161 actacttgct gaatcagttg cagtgggatg aatctgtcct gggtatgtat gaaaagctgg
    14221 gcaaggaggg tgatttaggt cggctttcag agcaagaggt ggtgttaacc cgctatttgg
    14281 gcgagaaact gatagagctc aaccctggct tgccggatgt cgcctaccaa gaggcgctgc
    14341 gcgtggtttg tgagatgcca gcatccacca atattgttgc tgtaaataag gagaaatact
    14401 cccttcataa gaatggcgta gaggtgagct tccataacga aaaaggtgaa cgggtaaaga
    14461 aacggcttcg cctgtttgat tttgacaact acgaaaacaa tcacttcttg ctggtcagag
    14521 agttttggat caaaggcgat atttatcgtc gcagagcaga ccttgtgggc tttgtgaatg
    14581 gtattccgct gctgtttatg gaggtgaaaa acgttcacaa ggatatccgt gctgcctatg
    14641 agcaaaatct tgcggactac aaagacaccg tcccgcacct gttttatcac aacgccttta
    14701 ttatcttggg caatggtatt gacgccaaga ttggctccgt ttccagtaaa tttgagcatt
    14761 tcaatgactg gaagcgtttg aatgaaagtg agcctggtgt ggtggatatg gaaaccttgt
    14821 tgaaaggtac ctgctccaaa gccaatctca tgaacatttt tgagaacttc actctgtttg
    14881 accagagctc gggccgcctg gtaaagatta ttgcccgcaa tcaccaatat cttggtgtca
    14941 gccgtgccat tgatgccgtg attaatcgtg gcgagcggat gggtaagttg ggagttttct
    15001 ggcataccca gggctctgga aagtcctatt cgattgtctt tttcgctcag atggtgcatc
    15061 gaaaactggg tgggaactat acctttgtgg tgctgactga ccgagaagat ctcgatacac
    15121 agatttataa aacctttgca ggctgtggct tggtcgacaa cgataaagac ccgtgccgcg
    15181 ccgatagtgg taaagacctg aaagagctga tcggccagca aaaggcgttt gtatttacct
    15241 tgattcaaaa atttaacgaa aaagtcgacc ctaaaaagcc ttattccgag cgcgacgaca
    15301 ttatcgtcat caccgacgaa gctcaccgca cgcaatatgg cacgttgtcg ttaaacatgc
    15361 gcaacgcctt gcccaatgcc agttttatcg gcttcaccgg aactccatta tttaaagatg
    15421 atgagataac caagaaggtt tttggtgatt acatctctac ctatgacttt cagcgggctg
    15481 tcgaggataa agcgacagtg cccttgtact acgatgcccg tggtgaagaa ttggtattta
    15541 ccgatgaaga tggcaacgag catacggttg cggaccccaa aggcatcaat gagcgcatag
    15601 cggaaaagct ggatgagttg gaaattgagg atgtagacgt tcagcaaagg ttggagcgag
    15661 agctgaagcg tgattatcac attatcactg caaccagccg actggatcag atagcacgtg
    15721 actttgtggc tcattatgcc aacgcttggg aaaccggcaa ggcgatgttt gtgtgcctcg
    15781 acaagatcac ttgtgtacgg atgcacaaat tgatcgattt ttactggcaa caggaaatta
    15841 aaaccaaaga aaaagcactt ttgtcggcta cggatgagca ggatctagct tggcgagagc
    15901 ggcaattggc gtggatgaaa gaaacgctga tggcggttgt ggtcagtgaa gagcaaggtg
    15961 aagtcgccaa gtttgaaaaa tgggggctgg atatcaagcc tcaccgccag ttgcttaaag
    16021 caggacttga actggctgat ggctctcgac tggacatgga agaagccttt aaatccagcg
    16081 aacacccttt tagagtcgcc gttgtctgtg ccatgtggct gaccggattt gatgtgccca
    16141 cactttctac cctctatttg gacaagcctt tgaaggcgca taccttgatg caagctatcg
    16201 ccagggctaa ccgagtagcg gaaggcaaga ataacggttt gatcgtggat tactgcggga
    16261 tactgaaaaa cctgcgtaaa gcattggcaa ccttcgccgg tgctggtgac gagggtcatg
    16321 gcggaggtga gggcgaaaat gaacccgcca agcccaatga agagttgtta gaaagtctga
    16381 acgagtcgat cagttttgtt gctgagtttt tacgaaagca tgattttgaa ctaagccgga
    16441 ttattacaga aacaggtttt gctaaaaatg cagcgattgc tcaagccaag gaaatcatca
    16501 atcaaaatga cgaaacacgg aaacgtttcg aggtaatggc cagagaggtg ttcaacaagt
    16561 ttaaagcctg cattaacgtg ccgggcgtta atggctatcg tgatatgcgc gatgccatca
    16621 acgtactcta taagagtttg caggcagata aagagaaagc cgacatctcc gatattatga
    16681 aggatctgta cgagattgtt gacggtagca tcgataccac ccataaagtc aaagaaccga
    16741 aaccggatgg tgatcgaatt tacgatatca gcaagatcga ttttgaacgg ctccgccagg
    16801 agttttctag atcagagcga aagaatacta cagtgcaatc gctcaagcat gcggttgaga
    16861 agaagctagc caggctcatg atgcagaacc cgttacgtac cgattatcaa gagcactacg
    16921 aaaaattggt taaggaatat aaccaagaaa aggatcgcgt tgttattgaa aaaacctttg
    16981 aggcactgtt aaaactgaat gaagagcttt ctcatgaaga gaagcgcgca atcagggaat
    17041 gcttggacga ggagtctttg gtattgtttg atctccttag taagccgaat ttgcagccaa
    17101 aagagattgc caagatcaaa caagtcgcca ctacgttgtt ggcaacgcta aaggcagagc
    17161 ggctgaaagt tgcaaactgg cagcagaaag aatcgacgcg cgacgcggtt aagcagcaga
    17221 tatttgattt tctttatgat gaaagaacag ggttacccgt tgagcaatat gaagaaaatg
    17281 agatttcaga tatcactgaa agggtgtttc ttcacatata tcgggcgtac cccgaactgc
    17341 catcgcccat ttatgggtga agaattacgc ggagttacgc atggtttgcg taattacgca
    17401 tatgatgcat aattgtgcgt aatttgaggc aactctatcc caaacctcaa ataggccggt
    17461 tacctgccgg atggtgcctt ggtctaatga gctcttagcc ggtgacggct tgaaagcggg
    17521 tgtaaatacc aaggtttgac atagagttga tggcaagagc gggaaatgga ttttatttcg
    17581 ccgagatctt gctaaaatgc gattcaatgt tcggtagaaa caaccagcct tccaataccc
    17641 gcctttttga ttttgggtgt tggagtcgcc tgagttgatc aggtgctgtt gacctgctcc
    17701 ccgttgatta gtacaccccg atgttagtaa tgtcttcata agccacatga ggacatcccc
    17761 atgaagaagc gtttttccga cgaacagatc atcagtattc tccgcgaagc cgaagctggg
    17821 gtacccgccg gtgaactctg ccgcaagcat gccatttccg atgccacgtt ttacacctgg
    17881 cgtaagaagt atggcggtat ggaggtgcct gaagttaagc gcctgaagtc gcttgaggaa
    17941 gagaacacca gactcaagaa gctgcttgcc gaagccatgc tggataaaga ggcgcttcag
    18001 gtggctcttg ggcgaaagta ctgacgacag accagaagcg ggaagccgtg atgttgatgt
    18061 gtgatgcgac cggtctgtcg caacgtcgtg cctgcaggct tacaggttta tccctgtcga
    18121 cctgccgcta tgaggctcac cgtccggctg ctgatgcgca tttatcaggg cgcatcactg
    18181 agctggcact ggagcgcagg cgttttggct accgtcgtat ttggcagttg ctgcgccgtg
    18241 aagggcttca tgttaatcat aagcgcgtgt accggcttta tcacctcagt ggtctgggcg
    18301 taaaacgcag aagacgtcgt aaagggctgg caacagaacg tctgccgctg ctccgtccgg
    18361 cggcgcccaa tctgacctgg tcgatggatt tcgtcatgga cgcactttcc accggtcgca
    18421 ggatcaagtg tcttacctgc gtcgatgatt tcacaaagga atgcctgacg gtcactgttg
    18481 cctttgggat ttcaggcgtt caggtcacgc gtattctgga cagcattgca ctgtttcgag
    18541 gctatccggc gacgataaga actgaccagg ggccggagtt cacttgccgt gcactggatc
    18601 aatgggcctt tgagcatggt gttgagttgc gcttaatcca gccgggcaag ccaacgcaga
    18661 acggatttat tgagagcttt aacggacgat ttcgcgatga atgtttgaat gagcactggt
    18721 tcagcgatat cgttcatgcc aggaaaatta ttaatgactg gcggcaggat tataacgaat
    18781 gccgcccgca ctccacgctg aattatcaga caccgtctga atttgcagcg ggctggagaa
    18841 agggtcattc tgagaatgaa gattccgacg ttactaactg agtgttgtat ctaatcgtgg
    18901 gggcaggtca ctgtcgctga aagtgtcaag attctatctt tacgcttggc ggctaatact
    18961 tccacagtac ctgcccagga agttgacaat attgggctta acttaacagc tttggctttc
    19021 tttatgataa aatccaatat tttcaatggg ttgaatggtt tgtttgctgc tttatgtgaa
    19081 ctaccgttct gatttgtctt taaaacaata gggtatattg ttttaaggtg gatgattaaa
    19141 gcatctgcca gccataaaaa agaagcctcc gttatggagg cttctacata tcaagtcaaa
    19201 atcattggcc attgtggcgg tgtctgattc atctcttcta tcatcaccga ctcgatgttg
    19261 tgccagatgg cggtgatatc cgccattgtg atgccaagca accccattgc gaaccagcag
    19321 gcggaaacta cgcccaaacc gctgctgatc actgcaagcc cgacataatc agcccgacga
    19381 aaacggatgt tgagcgtgct ggccagaaac atcattacgg cactaagaag ttgccagcga
    19441 agaagaacca cgccagtggt gagggtagcc atcaaacaat tcctcgaaaa agagctgatg
    19501 ccctggacag cgcggcgttg ttcacgggag gcgtgataca ctctggctat cgcggggctt
    19561 gcagaacaca aaaatgaaac actctgtttg tttcatgaat tttgtgaact atatcacaat
    19621 tggttgtttg ttagccagat taggccgtga cttttattgc cgtacagatt atgtgttttt
    19681 tcagcggcat taagggtata tcttcccgcc gcctctgcat tcctgaaggt gattaatttt
    19741 taagatcaat gaattatcgt catcctggtg acgattaaaa aggtctcaat tttatatcag
    19801 gcaaaagtgc tatttatacc gtaagattta tctaaagacg tcggtagcca gggtttcacc
    19861 ttgcaataac cgggtataaa caggcaggga attgatagca atgagtcatc gtgcacgaca
    19921 ccaattactg gcgtttccgg gcattatctt tttagttctc tttcccatca ttctgtcgct
    19981 atggattgcc ttcttttggg caaaatcaga agtgaataat cagctccgaa cttttgctca
    20041 gctggcgttg gataaatctg agctggtcat tcgtcaggca gatttagtga gcgatgcagc
    20101 tgaacgctat caggggcaag tttgcactcc agcccatcaa aagcgaatgt tgaatattat
    20161 tcgtggctat ctttatatta atgaattgat ttatgctcgt gataaccatt ttttatgctc
    20221 atcgctgata gcgtctgtaa atggctatac gattgcaccg gccgattata agcgtgaacc
    20281 taacgtttct atctattatt accgtgatac gccttttttc tcaggctata aaatgaccta
    20341 tatgcagcgg gggaattatg tagcggtcat taaccctctc ttctggagtg aagtgatgtc
    20401 tgatgatccg acattgcaat ggggtgtgta tgatacggtg acgaaaacct ttttctcgtt
    20461 aagcaacgag gcctcggcag caacgttttc tccgctgatt cgtttgaatg atttaaccgt
    20521 acaacgaaat ggctatttat atgcgacagt ttattcgaca aaacgcccaa ttgcggccat
    20581 tgttgcgact tcatatcaac gtcttataac ccatttttat aatcatctta tttttgcgtt
    20641 gcccgccggt attttgggga gtcttgttct gctattactc tggctacgta ttcgacaaaa
    20701 ctatttgtct cccaaacgca aattgcaacg cgccctcgaa aaacatcaac ttttccttta
    20761 ttaccagcca ataatcgata tcaaaacaga aaaatgtatc ggcgctgaag cgttgttacg
    20821 ttggcccggt gagcaggggc aagtaatgaa tccggcagag tttattccgc tggcagaaaa
    20881 ggaggggatg atcgaacagg taactgatta tgttattgat aatgtcttcc gcgatttggg
    20941 cgcgtatctg gcaacacatg ccgatcgcta tgtttctatt aacctgtcag cctccgattt
    21001 tcatacctca cggttgatag cgcgaaccaa tcagaaaaca gagcaacatg cggtgcgccc
    21061 gcagcaaatt aaatttgaag tgactgaaca tgcatttctt gatgtcgaca aaatgacgcc
    21121 gattattctg gctttccgca aggccggtta cgaagtggca attgatgatt ttggtattgg
    21181 ctactctaac ttgcataacc ttaaatcatt gaatgtcgat attttgaaaa tcgataaatc
    21241 gtttgttgag acgctgacca cccacaaaac cagtcatttg attgcggaac acatcatcga
    21301 gctggcgcac agcctggggt taaaaacgat tgctgaaggt gtcgaaactg aggcgcaggt
    21361 taactggctg cgcaaacgcg gcgtgcgcta ttgccaggga tggttcttcg cgaaggcgat
    21421 gccgccgcag gtgtttatgc aatggatgga gcaattaccc gcgcgggaat taacgcgcgg
    21481 gcaataaaac tacaggcggt ggcgataatc gctgggagtg cgatcaaact gccgacggaa
    21541 aacgcgggag aaggtctgct gagagacata acccaggtcc attgcgatat caaaaatcgg
    21601 acgctcggtg gtgcgcaact caacggcggc cagtaacaga cggcgctggc gaatgtaatc
    21661 gccaagcgtc tgatgcgtca ccgtgcggaa catccgttgc aagtaccact ttgaatagcc
    21721 agattttttt gcgacgacat caatgttaag cggctggtca atatgctcgt caatccatgc
    21781 gataagatcc tgaataattt tctgatggga cataaatctg cctcttttca gtgttcagtt
    21841 cgttaattca tctgttgggg agtataattc ctcaagttaa cttgaggtaa agcgatttat
    21901 ggaaaagaaa ttaccccgca ttaaagcgct gctaacaccc ggcgaagtgg cgaaacgcag
    21961 cggtgtggcg gtatcggcgc tgcatttcta tgaaagcaaa gggttgatta ccagtatccg
    22021 taatagcggc aatcagcggc gatataaacg tgatgtgttg cgatatgtcg caattatcaa
    22081 aattgctcag cgtattggca ttccgctcgc aaccattcgt gaagcgtttg gcgtgttgcc
    22141 agaagggcat acgttaagtg cgaaagagtg gaaacagctt tcgtcccaat ggcgagaaga
    22201 gttggatcgg cgcattcata ccttagtggc gctgcgtgac gaactggatg gatgtattgg
    22261 ttgtggctgc ctttcgcgca gtgattgccc gttgcgtaac ccgggcgacc gcttaggaga
    22321 agaaggtacc ggcgcacgct tactggaaga tgaacaaaac taaagcgcca caagggcgct
    22381 ttagtttgtt ttccggtctt tgtctttctc tctatcccgc tggtacacag gagggtttcc
    22441 cccgacgtca acacacctca ttcgagcacg tggtggaggt tccggttggt gttgatgctt
    22501 taattgtatg ttaccggcgt ttcttcgcca gtgtaaaagt acacttttta gccgcaatat
    22561 ttttgtcatc tcagacgatt ttttatcgca atcctgaacg gtatacggct cgataacgct
    22621 gcaatcttgc gcatcgacga taacgtttgc gcatcaattg cctggttttt catcgtcaag
    22681 acaataaaag agaaaaaagc agcaaacttc ggttgaaaaa gccgctatga tcgccggata
    22741 atcgtttgct ttttttacca cccgttttgt atgcgcggag ctaaacgttt gctttttttg
    22801 cgacgcagca aattgtcgca aacctggagc aggaagataa cgtttcgctg gcaggggatt
    22861 gtccgccacg catcttgacg aaaattaaac tctcagggga tgttttctta tgtctacgcc
    22921 atcagcgcgt accggcggtt cactcgacgc ctggtttaaa atttcacaac gtggaagcac
    22981 tgtccgtcag gaagtggttg ccgggttaac aacgtttctg gcgatggtct actcggttat
    23041 cgtcgttcca ggcatgttgg gtaaagcggg cttcccgcct gcggcagttt tcgttgcaac
    23101 ctgtctggtt gccggactcg gttctatcgt gatgggtctg tgggctaatc tgccgttggc
    23161 gattggttgc gccatctctc tgacggcgtt taccgcattc agtctggtgc tggggcaaca
    23221 tattagcgta cctgtcgcgc tgggtgccgt gttcctgatg ggtgtgctgt ttacggtaat
    23281 ttctgccacg ggtatccgta gctggatttt gcgcaacttg ccgcacggtg tggcgcacgg
    23341 cacggggatt ggtatcggtc tgttcctgct gctcattgcc gctaatggtg tcggtctggt
    23401 gattaaaaac ccgcttgatg gtctgcccgt cgcgctgggg gattttacga cctttccggt
    23461 gatgatgtcg ttggtggggc tggcagtgat tatcggcctg gaaaaactga aagtccctgg
    23521 cggcattctg ctgaccatta tcggtatctc aattgtcggt ttgatcttcg atcctaacgt
    23581 ccatttctcc ggcgttttcg ccatgccttc attgagcgat gaaaacggca attcactgat
    23641 aggcagcctg gacattatgg gcgcgctgaa tcctgtagtc ctgccaagtg ttctggcact
    23701 ggtgatgacg gcagtatttg atgccaccgg aactatccgt gccgtcgccg gtcaggcgaa
    23761 cctgctggat aaagatgggc agatcatcga cggcgggaaa gcactgacca ccgactccat
    23821 gagcagcgtt ttttctggcc tggtgggtgc agctccggca gcggtataca tcgagtctgc
    23881 ggcgggtacg gcggcgggcg gtaaaaccgg tttgacggct atcaccgttg gcgtgctgtt
    23941 cctgctgatt ctgttcctct ctccgctctc ttacctcgtt ccggggtacg caacggctcc
    24001 ggcgctgatg tacgttggcc tgctgatgct gagcaacgtg gcgaaaatcg actttgctga
    24061 ttttgttgat gcgatggcgg gtctggttac ggcggtattc atcgtgctga cctgtaacat
    24121 cgtaacaggc atcatgattg gcttcgcgac tctggtgatt ggtcgtctgg tttccggcga
    24181 atggcgcaag ttgaacatcg gtacggtcgt tattgccgtg gcgctggtga ccttctatgc
    24241 gggtggctgg gctatctaat cttacatagg attctgaaaa cgggtggcaa tggctgcccg
    24301 tttttatttt ctccgcacat tgtgtgagct tttgcgatat tctgaaaaaa tgagaattca
    24361 ggcataacgc ctttctcaga tcataaaaaa catcgcaaac agggaacgca tggaaatctt
    24421 cttcaccata ctgataatga ccctcgtggt ctcgctgtcc ggggtggtca ctcgtgtcat
    24481 gccctttcag atcccgcttc cgcttatgca aatcgccatc ggtgcgctac tggcgtggcc
    24541 gacgtttggt ttgcatgtgg agtttgatcc tgaactcttt ttagtcttgt ttatcccgcc
    24601 gttgctgttc gctgatggtt ggaaaacgcc gacccgtgaa tttctcgaac atgggcgaga
    24661 gattttcggc ctcgcgctgg cgctggtggt ggtcaccgtg gtcggcattg gcttccttat
    24721 ttactgggtg gtgccgggca ttccgctgat ccccgccttt gcgctggctg cggtgctttc
    24781 tccgaccgat gctgtagcgc tctccgggat tgttggcgag gggcgcatcc cgaaaaaaat
    24841 catgggcatt ttgcagggcg aagcgttgat gaatgacgcc tccggcctgg tgtcgttgaa
    24901 gtttgccgtg gcagtggcga tggggacaat gatcttcacc gtcggcggtg cgacggtcga
    24961 atttatgaaa gtggcaatag gcggtattct cgccggtttt gtggtgagct ggctgtatgg
    25021 tcgctcgctg cgattcctca gccgctgggg cggtgatgaa cccgcgacgc agatcgttct
    25081 gctgttcttg ctgccattcg cttcttatct gattgcggaa catattggcg tttccggcat
    25141 cctcgctgcg gttgccgccg ggatgaccat cacccgctcc ggtgtgatgc gccgtgcgcc
    25201 gctggcaatg cgcctgcgcg caaacagcac ctgggcgatg ctggaatttg tctttaacgg
    25261 catggtgttc ctgctgttag gtctgcagtt gccggggatt ctggagacgt cgctgatggc
    25321 ggcagaaatc gatcctaacg tcgaaatctg gatgctgttt accgatatta ttctgatata
    25381 tgcggcgctg atgctggtcc gtttcggctg gctgtggacg atgaaaaagt tcagcaaccg
    25441 cttcctgaag aagaagccga tggagtttgg ttcgtggact acacgagaaa tcctgatcgc
    25501 gtctttcgcc ggggtgcgtg gggcgatcac tctggccggt gtgctctcta tcccgcttct
    25561 cttgccggat ggtaacgtct tccctgcgcg ctatgagttg gtgttcctgg cggctggcgt
    25621 cattctcttc tcgttgtttg tcggcgtggt gatgttgcct attctgctac aacacataga
    25681 agtcgcggac cattcgcaac aattgaaaga ggaacgtatt gcacgagcgg caacggcaga
    25741 agtggcgatt gtggcgatcc agaaaatgga ggagcgtttg gcggcggata ccgaagagaa
    25801 tatcgataac cagctgctca cagaggttag ttctcgcgtc attggtaacc tgcgtcgtcg
    25861 cgccgatgga cgtaatgatg tagaaagttc aatcctggaa gagaacctgg agcgtcgctt
    25921 ccgtctggcg gcattgcgtt ctgaacgtgc tgaactgtac cacctgcgcg ccacgcggga
    25981 gatcagtaac gaaacgctgc aaaaattact gcacgatctc gatttgcttg aagcgttgct
    26041 gattgaggaa aatcagtaat ctgcatggtt ggcgggcgag cccgccaacc cgacttactc
    26101 tggcgttaac cagtatcctt cacaacagct cagccacgcg tgggcagagt gggaaaggta
    26161 aacgccctca cgccaaatca ttcctaattt ccagcttagt tcgctttcca gcggtagcca
    26221 ttttaaggtg tttttatcta atcgctggca aatgggttcc ggcaatatgg ctattccgac
    26281 accggcctga accatcgcgg cgagaaaatc ccattgcccg ctgcgaactg caattctcgg
    26341 cttaacgcca tgttcatcga ataacttcat caattgctta cttaaggcaa aatcttcgtt
    26401 atagataagt agaggatgtt ccgccaatgt ctccggtgag acggcatcac attcaagcca
    26461 tttacctgaa tgtggcgtca gtacacatag tggatgacta aacagcgata acgttgtcag
    26521 gccgctttct tctactggca gggctgtcag tgccatatcc agttcaccgt tcatcaccgc
    26581 ttgttgtacc gtcagaccac caaactccga aattttcaat tctacgccgg ggtaacgctg
    26641 gcgaaaaagg ccaatcggcc cggccatcaa catcccaacc atcggcggga ttcccagccg
    26701 caaaacgccg gttttaagct gattgatatc gctaagctca gcttccagtt gccgaaactc
    26761 agccagtaaa gccagcccgc gctcaaatac gacgcgacca gtatcagtga gaagcagccg
    26821 acgaccatcg cggatcaaca atatacagtt cagctcatct tcgagattct tcagcatctt
    26881 gctgatagtt ggttgggtga caaacaactt ctccgctgcg cgggtgaagc tttgctggcg
    26941 cacgacttcg acaaaataac gcagcgttct gatatccata actattccct ttgactatac
    27001 ctgcaatgat tttaattcat ttcagtcagg ggcgagcgct ctctatactt accattctgt
    27061 tttattttca ggatatcctc tcatgcccgt ggcgcttcgc cgcattaccc ctgccgtagt
    27121 gcaatctatc caggtatttt ttcaggttgt gctgtatgca ggtttattta tttttgcgca
    27181 gtatctcgtc tcctggctgc atttgccgct gcctgccaac ctggtgggga tgatcctgat
    27241 gctggcgctg attgtttgtc gcattcttcc tctgcaatgg gttcgagccg gggcgcgttg
    27301 gctattggcc gaaatgttgc tgttttttgt ccctgccgtg gtggctgtgg tgaactatgc
    27361 acaactatta ctggtggatg gctggcgtat ttttgctgtg atcgcgctga gtacggtgat
    27421 ggtgctgggc acgactgcct gggtagtgga aaaagtctat cgctatgaaa tgcgcaggtt
    27481 aaaccgtggc taattttcag ctaagtatgc tctgtctggc aattacgcta gttatctatt
    27541 ttcttaacaa acgcctttat cgccgctttc gtaaattgcc attgatgcct ctggtgctga
    27601 cgcctacgct actggttatg ctgctggttt ttggcaatat ttcctggcaa aactatattg
    27661 gcgaagcgca ctggttacta tggctattag gtccggccac catcgccttt gcggtaccag
    27721 tgtatgacaa tcgggcgatc attaagcgtc actggatgtc gttgacggca ggcgtactga
    27781 cggcaacggt ggtggcagtg accagctcag tgtggctggc gcgattgttt accctgccag
    27841 atgagatcca gcgtagcctg gcggtgcgtt cggtcactac gccatttgct ttggcagcga
    27901 gccagtcgct cggcggacag cctgatctgg tggcgctgtt tgtggtggtc accggtgtgt
    27961 ttggcatggc gattggcgac gtgttgtttt tacgtctctc tattcgcgaa gggatggcga
    28021 aaggtgctgg attcggcgcg gcatctcatg gtgccgggac tgcgcggtcg tatgaactgg
    28081 gtcaacaaga aggcgtagtt gcaagcctgg tcatgatgct ttcaggtgta ttgatggtat
    28141 tggtttcgcc gctggtggcc aggatgatgt tctgaagaaa ttccccgatc aagcgaccgg
    28201 ggaaggggag agattaatgc gcgcggccct gctcaacgcc aaagccagtc tgggagcgga
    28261 taaactgcgc acggaacagt tcacgctcgc gcgcaccttc cgctgaatta tccgttgccg
    28321 agaagaacca gataccgagg aatgccacgc tgatagagaa cagcgccggg tattcatacg
    28381 ggaaaatggc tttttcgtga ccgaggatct gtacccaaat cgtcgggccg aggatcatca
    28441 gcaccaccgc agtaatcagt cctaaccagc cgcccagcat tgcgccacgg gtagtcagtt
    28501 tcgaccagta catggaaagc agaatgatcg ggaagttaca gctcgccgcg atggcaaacg
    28561 ccagccccac cataaaggcg atgttctgat tctcaaacag cacgccgagg ataatggcaa
    28621 tcacaccgag gatcagtacg gtgattttcg atacccgcag ctcttcacgt tcggtcgcac
    28681 cttttttgaa gacgttagcg tacaggtcgt gagacaccgc cgatgcgccc gccagcgtca
    28741 gacccgcaac caccgcaaga atggtggcga aagcaaccgc tgaaataaag ccgaggaaca
    28801 ggttgccgcc caccgcattg gccaggtgaa ctgctgccat gttgttacca ccaatcagat
    28861 ggcccgccgc gtctttatat tccggattcg caccgaccag catgatcgcg ccaaagccga
    28921 taataaaggt caggatgtag aagtagccca taaatccggt ggcgtagaac acgctcttac
    28981 gtgcttcgcg ggcatcgctg accgtaaaga agcgcatcag aatatgcggc aagcccgccg
    29041 taccaaacat cagtcctagc cccaaagaga gcgcggagat aggatctttc accagcccgc
    29101 ccggcttcat gatgtcgaca cctttcgggt gtaccgccat cgcttcgctg aacagattgt
    29161 tgaagctaaa gccgacgtgt ttcatcacca taaaggccat aaagctggca ccgaacagca
    29221 acagcacggc tttaataatt tgtacccagg tggtcgccag catgccgccg aacaggacgt
    29281 acatcatcat cagcacgccg accagcacta ccgcaatgtg atagttaagg ccaaacagca
    29341 gctcgatcag tttacctgcg cccaccatct gggcgataag gtaaagcgcc accaccacca
    29401 gagaaccaca ggccgaaaga atacgaatcg gcccttgttt cagacggtaa gaggccacat
    29461 cggcaaaggt gtagcgaccg aggttacgca gacgttcggc aatcaggaac agaatgatcg
    29521 gccagcccac caggaagccc agcgagtaaa ttaagccgtc atagccggag gtaaacacca
    29581 gcgcggagat ccccaagaat gaggcggcgg acatatagtc cccggcaatc gccagcccgt
    29641 tctgaaaacc ggtgatattg ccgcctgcgg tgtagtagtc attacgcgaa cgtacgcgtt
    29701 ttgacgccca gtaggtaatg ccgagcgtaa acacgacgaa aatcaggaac ataataatcg
    29761 cctgccagtt cgttggctgg cgctctacgg ccccgctaat agcatccgcg gcgttagctg
    29821 cgaaagggag tgtggcggca agcgccgtca gaactctttt catgatgctt gtacctcatg
    29881 caggacttca ttattaagac ggtcgaattc gccgttcgcc cgccagatgt agataccagt
    29941 gagaacaaag gagatcacaa ttaccccgac accgactgga ataccgcgtg tgacgctggt
    30001 gttcggattc agcggggtgc ccagccagcc gggcgcgaag gcgatcagta aaataaagcc
    30061 gatataaact gccagcataa taatcgacag gatggtggca aaccgttgcc gtttttcgac
    30121 taactccctg aaatgcgcat tgtcttctat ccgctgataa atcgtaccat tcatcacaga
    30181 ttctccagag gtaatgtagg gattgtttta attcccgctc ccttatggga gaaggttaac
    30241 gcttgggtaa cccttgccga atgtaggccg gataaggcat tcacgccgca tccggcgctt
    30301 gttgtcggat gcgatgctgg cgcatcttat ccgacctaca gccgttgcca gatgtaggtc
    30361 ggagcgcatc cgacaattgt gggttacgat ggcatcgcga tggcctgctt ctcttcaagc
    30421 agcttctcga ctacgccagg atcggcaagc gtcgaggtat cgcccaggtt gctggtatcg
    30481 cccgccgcaa ttttgcgcag aatacggcgc ataattttgc cggagcgggt tttaggcagg
    30541 gagtcggtcc agtgcagcac gtctggcgtc gccagcgggc caatctcttt acgcacccag
    30601 ttgcggactt ctgcgtacag ttctggtgac ggttcctccc cgtgattaag cgtgacgtag
    30661 gcgtagatcg cctgaccttt aatattgtgc ggaataccga ccacagcggc ttcggcaatt
    30721 ttcggatgcg ccaccagcgc cgactcaatc tctgccgtcc ccaggcggtg accggagacg
    30781 ttcagcacgt cgtccacgcg cccggtgatc cagtaatagc catcttcatc acgacgcgcg
    30841 ccgtcgccgc tgaaatacat atttttgaag gtggagaagt aggtctgctc aaaacgttcg
    30901 tgatcgccaa acagcgtacg cgcctgaccc ggccaggagt cggtgatcac cagactgcct
    30961 tcggtggccc ccttcagcgg gttaccttcg ttatcgacca gcgccggttg cacgccgaag
    31021 aacggacgtg ttgccgaacc ggctttcagc tcggtagcgc caggcagtgg tgtgatcatg
    31081 aaaccgccag tttcggtctg ccaccaggtg tcgaccaccg ggcatttctc gttgccgatc
    31141 ttcttccagt accactccca cgcttccggg ttaattggct cgcccacgga accgagaatg
    31201 cgcagcgacg aacggtcggt gccttcgatc gctttatcgc cttccgccat cagcgcgcga
    31261 atcgccgtgg gcgcggtata gagaatattg acctgatgct tgtccaccac ctgcgccata
    31321 cgggcaggcg tcggccagtt cggtacgcct tcaaacatca gcgtggtcgc gccgcaggcc
    31381 agcgggccat acagcaaata actgtgtccg gtaacccagc ccacatcggc ggtgcaccag
    31441 tagatgtcgc ccggatgata atcaaagaca tatttaaagg tcagcgccgc gtacaccaga
    31501 taaccgccgg tagtgtgcag cacgcctttt ggcttaccgg tagaaccgga ggtatagaga
    31561 ataaacagcg gatcttcggc gttcatctct tccgcctggt gctgatcgct ggcttgctca
    31621 accaggtcgt gccaccacag gtcgcgccct tcctgccagt caatttttcc gccagtacgc
    31681 ttcagtacca ccacatgctc tacgctggtg acgttcgggt ttttcagcgc gtcatcaacg
    31741 tttttcttca gcggaatact acgcccggca cgcacacctt cgtcggaagt gatcaccagt
    31801 cgtgaactgg agtcgataat gcgtccggca acggcttccg gcgagaagcc gccgaaaatc
    31861 accgaatgta ccgcgccaat gcgggcgcag gccagcatcg cgaccgcggc ttccggtacc
    31921 atcggcatat aaatcgcaac tacgtcacct tttttaatgc ccagttcgag cagggtattg
    31981 gcgaagcggc agacgtcgcg gtgcagctct ttatagctaa tatgtttgct ctggctggcg
    32041 tcgtcgcctt cccagatgat ggcggtacga tcgccgtttt cttgcagatg gcggtcaagg
    32101 cagtttgccg ccagattcag cgtgccgtcc tcgtaccatt taatggacac attaccgggg
    32161 gcaaaggagg tgtttttcac cttctggtac ggtgtgatcc agtcgagaat ttttccctgt
    32221 tcgccccaga aggtatctgg tgcgtcaata gattgttgat acatcgcctc gtactgctga
    32281 gggtttatca ggcaacggtc tgcgatgttg gcaggaatgg tgtgtttgtg aatttgactc
    32341 atgcttttgt tctccttgta ggatgttaat aatatgtggc ataagcgtta aatgtagggg
    32401 tattggcagt tttgtttagt atttgggcga cagatcacgc aaaagtagaa ttgtgcaaat
    32461 aaacggcagg gtaatttttg aaggtcagca acaaaagttg attaattctt tgaggaacat
    32521 gcagttatgc atgttgttga aaaagaggaa gataccgact aactctaaag tggtatttta
    32581 catgcactta caattgatta aagacaacat tcatagtgtg gttatttgtt acacataggg
    32641 gcgagcaatg tcatgacagt gtaggtgcgg ttactgtcgt gaaaaacaat aaaaaccgcc
    32701 attgcaacaa tggctcaatt cggatgaagc cctatgacaa ggataaaaat aaacgcacgc
    32761 cgtatcttca gcttattgat tccttttttc tttttcactt ctgttcacgc tgaacaaacg
    32821 gctgctcccg caacacctgt aactgtggaa gcgaagaatg aaacctttgc cccgcagcat
    32881 cccgatcaat atctctcctg gaaagctacc tcggaacagt cagagcgtgt tgacgccctg
    32941 gcggaagatc cacggctggt gatcctgtgg gcggggtatc ccttctcgcg cgattacaac
    33001 aagccgcgtg gacatgcctt tgctgtgacc gatgtgcgtg aaaccttgcg taccggtgcg
    33061 ccgaaaaacg ctgaagatgg tccgctaccg atggcgtgct ggagttgtaa aagcccggat
    33121 gtggcgcgtc tgatccagaa agacggcgaa gatggctact tccacggtaa atgggcgcgc
    33181 ggcggcccgg aaatcgtcaa caacttaggt tgtgccgatt gccataacac cgcctcacca
    33241 gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc cgtatgcggc tcgcgcgatg
    33301 gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg accagcaatc gatggtttgc
    33361 ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca aagcggttaa attcccgtgg
    33421 gatgacggca tgaaagtcga aaatatggag cagtattacg acaaaattgc cttctctgac
    33481 tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc agcacccgga atatgaaacc
    33541 tggacagcgg gcattcacgg caaaaacaac gtgacctgta tcgattgcca tatgccaaaa
    33601 gtgcagaacg ccgaaggcaa actctacacc gaccataaaa ttggtaatcc gtttgataac
    33661 ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag ctgccttgca aaaagtggtc
    33721 gcggaacgta agcagtcgat taacgacctg aaaatcaagg ttgaagatca actggttcac
    33781 gctcacttcg aagcgaaagc ggcgctggat gcaggcgcaa cggaagccga aatgaagccg
    33841 attcaggacg atatccgtca tgcccagtgg cgttgggatt tggcgatcgc ttcccacggc
    33901 attcatatgc acgcaccgga agaaggtctg aggatgctcg gtacggcgat ggataaagcg
    33961 gcggatgcac gcaccaaact ggcgcgcctg ctggcgacca aaggcattac ccatgaaatc
    34021 cagatcccgg atatctcgac caaagagaaa gcccagcagg ccattggcct gaacatggag
    34081 caaatcaagg ccgagaagca ggacttcatc aaaacggtga tcccgcagtg ggaagagcag
    34141 gcacgtaaaa acggtctgtt aagccaataa ccccgttccg cctcgcaagg ggcggaaaac
    34201 acaatggagt gaatatgagc gtattacgtt cgttattaac tgccggggtg ctggcgtcgg
    34261 gcctgttgtg gagcctgaac ggaattaccg ctacccctgc ggcgcaggca tctggtgatc
    34321 gctacgaagt tacccagcag cgtaacccgg atgccgcctg tctggactgt cataagccag
    34381 ataccgaagg tatgcatggc aaacatgctt ccgttatcaa cccgaataac aaactgccgg
    34441 tcacctgcac caactgtcac ggccagccat caccgcaaca ccgcgaaggg gtgaaagatg
    34501 tgatgcgctt taacgagccg atgtacaagg ttggagagca gaacagcgtc tgtatgtcct
    34561 gccatctgcc ggaacagttg caaaaagcat tctggccgca cgatgtccac gtaaccaaag
    34621 tggcgtgcgc cagctgccat tccctgcatc cacagcaaga tacgatgcag acgttaagtg
    34681 acaaaggacg gattaagatt tgcgtcgatt gccacagcga tcagcgcacc aatccgaact
    34741 ttaacccggc gtctgttccg ttgcttaagg agcagccatg acctggtctc gtcgccagtt
    34801 tctcaccggc gtcggtgtgc tggccgccgt cagcggcacc gcagggcgcg tggtggcgaa
    34861 aacgttgaat atcaatgggg tgcgttacgg catggtgcat gacgagtcgt tatgcatcgg
    34921 ctgtaccgcc tgtatggatg cttgtcggaa agtgaacaag gtgccggaag gcgtctcgcg
    34981 cctgacgatt attcgtagcg agccgcaggg cgaatttcct gatgtgaaat atcgcttctt
    35041 ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt gacgtctgcc cgaccggtgc
    35101 gtcttttcgc gacgctgcca gtgggattgt cgatgttaat ccggatctct gcgtcggttg
    35161 tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt atccatccgg tcacgaagac
    35221 ggcggacaaa tgcgatttct gccgtaagac ccatttgcag gccggtaagc tgcccgcgtg
    35281 cgttgaagct tgcccgacga aggcgctgac gtttggcaat ctggacgatc ccaacagtga
    35341 gatttcgcaa ctgctgcgcc agaagcccac ttaccgctac aagctggcgc tgggcaccaa
    35401 accgaagctg taccgcgtac cgtttaaata cggggaggtg agccaatgac gcagacttcc
    35461 gcatttcatt ttgaatcgct ggtgtgggac tggccgattg ccatctacct gtttttgatt
    35521 ggtatttctg ccggtctggt gacactggcc gtgctgttac gtcgcttcta cccgcaggca
    35581 ggcggtgcag acagtacgtt gctgcgcacc acgctgattg tcgggcctgg cgcggtgatc
    35641 ctcggtctgt tgatcctcgt cttccacctg acaagaccgt ggaccttctg gaagctgatg
    35701 ttccactaca gttttacctc ggtgatgtcg atgggggtga tgctgtttca gctttacatg
    35761 gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg atttgcttgc cctgcaacaa
    35821 cgctggttgc cgaagctggg gatcgtgcaa aaggtcttga gcctgctgac gcccgttcat
    35881 cgcggactgg aaacattgat gctggtgctg gcggtgttgt tgggggctta taccggcttt
    35941 ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc cgatcctgcc ggtgctgttc
    36001 ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga tcgccatggc gatacgccaa
    36061 cgcagtaacc cgcattccac ggaagcgcac tttgtacacc gtatggaaat ccccgtggta
    36121 tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc tggcgctggg cgatgacggt
    36181 aaagtgcgtg cgctggtggc ggcattgggc ggcggtttct ggacgtggtg gttctggctt
    36241 ggtgtcgccg ggctggggct gattgtgcca atgttgctca aaccgtgggt caatcgcagt
    36301 tccggcattc ctgccgtgct ggcggcatgt ggggccagcc tggtcggcgt gttgatgctg
    36361 cgctttttca ttctctacgc cgggcagtta acggtggcgt aagccagaac agaggtggtt
    36421 tctggacgta ttccttcctg aagtcggttt tctggcgttg ttgttaagtc tcggggtcaa
    36481 cgtgttgatc ccgttgacgg ccttcgcggg agtgcggttg cgctggcctg ccatgatgcg
    36541 actcacttgc atcggcattc tggcgcagtt cgcgctcctg ctgctcgcct ttggcgtact
    36601 gacgtattgt tttctcatca gcgatttctc ggttatttat gtcgcccaac atagctatag
    36661 cctgctgtcg tgggaactca aactggcggc ggtgtggggc ggtcatgaag gttcgctgtt
    36721 gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt gcctggcatt accggcagca
    36781 aaccgatccg ctatttccgc tgacgctagc cgttttatct ctcatgctcg ccgcactgct
    36841 actgtttgtg gtgctgtggt ccgatccctt cgtgcggata tttccaccag caatcgaagg
    36901 ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc tttcatccac cgctgcttta
    36961 tctcggctac ggcggtttga tggtggcggc gagcgtggcg ctggcgagtt tactgcgcgg
    37021 cgagtttgat ggtacctgcg cccgaatttg ctggcgttgg gcactacctg gctggagcgc
    37081 attaacagcg gggatcatcc tcggttcctg gtgggcctat tgcgaactgg gctggggcgg
    37141 ctggtggttc tgggatccgg tggaaaacgc ctctttacta ccctggcttt ctgccacagc
    37201 gctgctgcac agtttatccc tgacacgcca gcgggggatt ttccgccact ggtcgctgtt
    37261 gctgacgata gttactctga tgctgtcgct gctgggcacg ttgattgtcc gttctggcat
    37321 tctggtttcg gttcatgcgt tcgcgctgga taacgtccgc gccgtgccgt tgttcggcct
    37381 gtttgtactg attagccttg cgtctctggc tctgtatggc tggcgagcgc gggacggtgg
    37441 cccggcggtg cgtttttcgg ggttatcgcg ggaaatgtta atcctcgcta cgctgttgct
    37501 gttttgcgca gtgctactga tcgtgctggt gggaacgctt tatccgatga tttacggcct
    37561 gctgggctgg ggacgcctct ccgttggcgc gccgtatttt aaccgcgcga cgttaccgtt
    37621 tgctctgttg atgctggtgg tgattgtgct ggcgacgttt gtctctggca aacgcgcgcg
    37681 gcttccgacg ctgctggcgc atgcgggcgt gctgttattt gccgcgggga tcgtggtttc
    37741 cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg ggtcagcagg tgacgctggc
    37801 aggatacacc ttccgttttg agcgcctcga tctgcaagcc aaaggcaatt acaccagcga
    37861 aaaagcgata gtggcactgt ttgaccatca gcaacgcatt ggtgaattaa cgccggagcg
    37921 gcgtttttac gaagcacgcc gtcagcaaat gatggaaccc tcgattcgct ggaacggcat
    37981 ccatgactgg tatgcggtca tgggagagaa aaccggagcg gatcgttacg catttcgttt
    38041 gtatgtacaa agcggtgtgc gctggatctg ggggggagga ttgttgatga ttgcgggcgc
    38101 attgttaagc ggatggcggg gaaggaagcg cgatgaataa agggcttctc acgctactgc
    38161 tgttatttac ctgttttgcg cgcgctcagg ttgtagacac ctggcaattc gctaatccgc
    38221 aacaacagga acaggcgtta aatattgcca gccagttacg ttgtccgcag tgccagaatc
    38281 agaacttact ggaatccaat gcgccggtgg cagtcagtat gcgccatcag gtttacacca
    38341 tggtggaaga ggggaaaagt gaagtcgaaa tcattggctg gatgaccgaa cgctacggag
    38401 attttgttcg ctataaccca ccgttaacgg gtcagactct ggtgttgtgg gcgctgccag
    38461 tagtgttgtt actgctgatg gcactgatcc tctggcgagt gagggcgaag cgatgaaaca
    38521 gccccaaata ccggtgaaaa tgctgacaac actcacgatt ttgatggtat ttctctgtgt
    38581 cggcagttat ctgttaagtc caaaatggca ggcggtacgt gcggagtatc agcgtcagcg
    38641 cgatccgctg catcagtttg ccagccagca aaccccggaa gcgcagcttc aggcattgca
    38701 ggataaaatc cgtgctaatc cacaaaacag cgaacagtgg gcgttactgg gcgagtatta
    38761 tctgtggcaa aacgattaca gcaattcgct gctggcgtac cgtcaggcgt tgcaactacg
    38821 tggtgagaac gctgaactgt atgcggcgct ggcgacggtg ctgtattacc aggccagcca
    38881 acatatgacc gtccaggctc gcgcaatgat cgacaaagcc ctcgcgctgg actctaacga
    38941 aatcaccgct ctgatgctgc tggcttccga tgcgtttatg caggcgaatt acgcgcaagc
    39001 catcgaacta tggcaaaaag tgatggatct caactcaccc cggatcaacc ggacacagct
    39061 ggtcgagtcg attaatatgg cgaaattgtt gcagcggaga tcagattaat catctggtga
    39121 tgaagattat gttattgagc tgcataaa
//
LOCUS       DACADF010000024        37734 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.024, whole genome shotgun sequence.
ACCESSION   DACADF010000024 DACADF010000000
VERSION     DACADF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.024"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            138..764
                     /locus_tag="ISB30_001230"
     CDS             138..764
                     /locus_tag="ISB30_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAP1988885.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKESQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            785..2887
                     /locus_tag="ISB30_001231"
     CDS             785..2887
                     /locus_tag="ISB30_001231"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HAP1988886.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2900..3538
                     /locus_tag="ISB30_001232"
     CDS             2900..3538
                     /locus_tag="ISB30_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAP1988887.1"
                     /translation="MNHQDELPLAKVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3602..4270
                     /locus_tag="ISB30_001233"
     CDS             3602..4270
                     /locus_tag="ISB30_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP1988888.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVIDNDNGTQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGVCPYQVRYLNPVTKVADKCDFCAETRLA
                     KGFPPICVSACPEHALIFGHEDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4267..5052
                     /locus_tag="ISB30_001234"
     CDS             4267..5052
                     /locus_tag="ISB30_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAP1988889.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHSERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLDRAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5056..5868
                     /locus_tag="ISB30_001235"
     CDS             5056..5868
                     /locus_tag="ISB30_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988890.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERLFCSQFVRSDDHARLGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGARARVAVTGNSSRISSAGDSGRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGCAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5880..7484)
                     /locus_tag="ISB30_001236"
     CDS             complement(5880..7484)
                     /locus_tag="ISB30_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAP1988891.1"
                     /translation="MKTIAIVGAGPTGIYTLFSLLQQQTPLSISIFDQADEAGIGMPY
                     SDEENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNLNLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVTFHRDNANEKLNITLMSRTGILPEADFYCPIPYEPLHIITDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEENL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPNVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGITA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7610..7915)
                     /locus_tag="ISB30_001237"
     CDS             complement(7610..7915)
                     /locus_tag="ISB30_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAP1988892.1"
                     /translation="MATLLQLHFSFNGPFGDTMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNVPLSQINQAKLA"
     gene            complement(8024..8100)
                     /locus_tag="ISB30_001238"
     tRNA            complement(8024..8100)
                     /locus_tag="ISB30_001238"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8064..8066),aa:Val,seq:gac)
     gene            complement(8105..8181)
                     /locus_tag="ISB30_001239"
     tRNA            complement(8105..8181)
                     /locus_tag="ISB30_001239"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8145..8147),aa:Val,seq:gac)
     gene            8489..9745
                     /locus_tag="ISB30_001240"
     CDS             8489..9745
                     /locus_tag="ISB30_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988893.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEACNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFGIGNINL
                     NGGKLWLITDAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9786..11159)
                     /gene="mdtK"
                     /locus_tag="ISB30_001241"
     CDS             complement(9786..11159)
                     /gene="mdtK"
                     /locus_tag="ISB30_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAP1988894.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
     gene            11374..12015
                     /gene="ribE"
                     /locus_tag="ISB30_001242"
     CDS             11374..12015
                     /gene="ribE"
                     /locus_tag="ISB30_001242"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAP1988895.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12055..13203)
                     /gene="cfa"
                     /locus_tag="ISB30_001243"
     CDS             complement(12055..13203)
                     /gene="cfa"
                     /locus_tag="ISB30_001243"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAP1988896.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13494..14705)
                     /locus_tag="ISB30_001244"
     CDS             complement(13494..14705)
                     /locus_tag="ISB30_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAP1988897.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14818..15750
                     /locus_tag="ISB30_001245"
     CDS             14818..15750
                     /locus_tag="ISB30_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1988898.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALKLENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15747..16772)
                     /gene="purR"
                     /locus_tag="ISB30_001246"
     CDS             complement(15747..16772)
                     /gene="purR"
                     /locus_tag="ISB30_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAP1988899.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQSHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17071..17160
                     /gene="ynhF"
                     /locus_tag="ISB30_001247"
     CDS             17071..17160
                     /gene="ynhF"
                     /locus_tag="ISB30_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAP1988900.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17326..18495
                     /locus_tag="ISB30_001248"
     CDS             17326..18495
                     /locus_tag="ISB30_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1988901.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFLSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHVTPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAIVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
     gene            complement(18641..19222)
                     /gene="sodB"
                     /locus_tag="ISB30_001249"
     CDS             complement(18641..19222)
                     /gene="sodB"
                     /locus_tag="ISB30_001249"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAP1988902.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKPLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19350..20177)
                     /locus_tag="ISB30_001250"
     CDS             complement(19350..20177)
                     /locus_tag="ISB30_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAP1988903.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTQKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20512..20859
                     /gene="grxD"
                     /locus_tag="ISB30_001251"
     CDS             20512..20859
                     /gene="grxD"
                     /locus_tag="ISB30_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAP1988904.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21231..21878)
                     /gene="rnt"
                     /locus_tag="ISB30_001252"
     CDS             complement(21231..21878)
                     /gene="rnt"
                     /locus_tag="ISB30_001252"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAP1988905.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAAEV"
     gene            complement(21981..22388)
                     /gene="gloA"
                     /locus_tag="ISB30_001253"
     CDS             complement(21981..22388)
                     /gene="gloA"
                     /locus_tag="ISB30_001253"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP1988906.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(22469..23566)
                     /gene="nemA"
                     /locus_tag="ISB30_001254"
     CDS             complement(22469..23566)
                     /gene="nemA"
                     /locus_tag="ISB30_001254"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAP1988907.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(23603..24202)
                     /locus_tag="ISB30_001255"
     CDS             complement(23603..24202)
                     /locus_tag="ISB30_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1988908.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            24305..24544
                     /locus_tag="ISB30_001256"
     CDS             24305..24544
                     /locus_tag="ISB30_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAP1988909.1"
                     /translation="MTEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            24593..25489
                     /locus_tag="ISB30_001257"
     CDS             24593..25489
                     /locus_tag="ISB30_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP1988910.1"
                     /translation="MVQRITIAPQGPEFSHFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            25570..26091
                     /gene="sodC"
                     /locus_tag="ISB30_001258"
     CDS             25570..26091
                     /gene="sodC"
                     /locus_tag="ISB30_001258"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAP1988911.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALIVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(26092..28104)
                     /locus_tag="ISB30_001259"
     CDS             complement(26092..28104)
                     /locus_tag="ISB30_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP1988912.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTVARIISPLRPTNVTDYRHIAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(28104..28961)
                     /locus_tag="ISB30_001260"
     CDS             complement(28104..28961)
                     /locus_tag="ISB30_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP1988913.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(28964..29200)
                     /locus_tag="ISB30_001261"
     CDS             complement(28964..29200)
                     /locus_tag="ISB30_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAP1988914.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            29401..29835
                     /gene="slyA"
                     /locus_tag="ISB30_001262"
     CDS             29401..29835
                     /gene="slyA"
                     /locus_tag="ISB30_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAP1988915.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(29882..30349)
                     /gene="slyB"
                     /locus_tag="ISB30_001263"
     CDS             complement(29882..30349)
                     /gene="slyB"
                     /locus_tag="ISB30_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAP1988916.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            30623..31732
                     /gene="anmK"
                     /locus_tag="ISB30_001264"
     CDS             30623..31732
                     /gene="anmK"
                     /locus_tag="ISB30_001264"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAP1988917.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATVAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            31830..32159
                     /gene="mliC"
                     /locus_tag="ISB30_001265"
     CDS             31830..32159
                     /gene="mliC"
                     /locus_tag="ISB30_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAP1988918.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            32218..32874
                     /gene="pdxH"
                     /locus_tag="ISB30_001266"
     CDS             32218..32874
                     /gene="pdxH"
                     /locus_tag="ISB30_001266"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAP1988919.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            33003..34277
                     /gene="tyrS"
                     /locus_tag="ISB30_001267"
     CDS             33003..34277
                     /gene="tyrS"
                     /locus_tag="ISB30_001267"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP1988920.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            34339..35199
                     /gene="pdxY"
                     /locus_tag="ISB30_001268"
     CDS             34339..35199
                     /gene="pdxY"
                     /locus_tag="ISB30_001268"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAP1988921.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(35243..35848)
                     /gene="gstA"
                     /locus_tag="ISB30_001269"
     CDS             complement(35243..35848)
                     /gene="gstA"
                     /locus_tag="ISB30_001269"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAP1988922.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(35954..37456)
                     /gene="dtpA"
                     /locus_tag="ISB30_001270"
     CDS             complement(35954..37456)
                     /gene="dtpA"
                     /locus_tag="ISB30_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAP1988923.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         9642 a         9088 c         9812 g         9192 t
ORIGIN      
        1 ttaaatcttt tctcatacac tatcgggtta gggaaaatat ctggtgttga ggacagcgtc
       61 agcagcgtat tagttgagtg cttaatatca ggctgttgaa ttgtcagcac tgtgtttgcg
      121 aagaggatgc gaaataaatg aacccggttg atcgtccact attaaatatt ggcttaacgc
      181 gattagaatt cttgcgtata tcaggaaaag gccttgctgg tttaacaatt gcccctgctt
      241 tactgtcact tttgggttgc aaacaggaag atattgatag cggcacagta gggttgataa
      301 atacacccaa aggggtgctg gtgacccaac gtgcgcgatg cacgggttgc caccgctgtg
      361 aaatctcttg taccaacttc aacgatggct cagtagggac attcttctcc cgtatcaaaa
      421 tccatcgcaa ttatttcttt ggcgacaacg gggttggctc tggcggtggc ctgtatggcg
      481 atctcaacta taccgcggat acctgccgtc aatgcaaaga gtcgcaatgc atgaacgtct
      541 gcccgattgg tgcgattacc tggcagcaga aagaaggctg tattaccgtc gatcataaac
      601 gttgtattgg ctgtagcgcc tgtactacgg catgcccgtg gatgatggcc accgtaaata
      661 ccgaaagtaa aaaatcctcg aaatgtgtgt tatgcggtga atgcgcaaac gcctgcccaa
      721 caggggcgtt aaaaattatc gagtggaaag atattactgt gtgaatcttg caaaggaaaa
      781 aatcatggct aacggttgga caggtaatat attaagagtc aatctcacga caggaaatat
      841 taccctcgaa gattccagta aatttaaaag ttttgtcggt ggcatgggct tcggctacaa
      901 aattatgtat gacgaagtac cgccaggcac gaaacctttc gatgaagcga ataaattagt
      961 ttttgctacc ggcccattaa ctggatctgg tgccccctgt agttctcgcg taaatatcac
     1021 ctcactttct acttttacca aaggaaattt agtcgtcgat gcccatatgg ggggcttttt
     1081 tgcagcgcaa atgaaattcg ctggatacga cgtcattatt atcgaaggga aagcgaaatc
     1141 accggtatgg ctgaagatta aagatgacaa agttagcctt gaaaaagccg atttcttatg
     1201 gggaaaaggg acgcgcgcaa cgacggaaga aatttgtcgc ttgaccagtc cggaaacctg
     1261 tgtggcggct attggtcagg ctggagaaaa ccttgttcct ctctctggca tgttgaatag
     1321 ccgtaaccac agcggcggtg cgggaactgg cgcaataatg ggttcgaaaa acctgaaagc
     1381 gattgcggtt gaagggacga aaggggtcaa cattgccgat cgtcaggaga tgaagcgtct
     1441 caacgattac atgatgactg aacttatcgg tgcgaataac aaccatgtcg tgccaagtac
     1501 gccgcaatcg tgggcagagt attcagatcc caagtcacgt tggacagcac gtaaagggct
     1561 gttttggggc gcggctgaag gtggtccgat tgaaacgggg gaaattccgc caggcaatca
     1621 gaatacggtc ggctttcgta cctataaatc cgtttttgac ttaggtccgg cggcagagaa
     1681 atacacagta aaaatgagtg gctgccactc ttgcccgatc cgttgtatga cccaaatgaa
     1741 tattcctcgg gtgaaagagt ttggcgtgcc cagcacaggt ggtaacactt gtgtagcaaa
     1801 ctttgtccat accaccatct tcccgaacgg gccgaaagat tttgaagata aggacgatgg
     1861 tcgtgtgatt ggtaacctgg ttggtctgaa tttgttcgat gactacggcc tatggtgtaa
     1921 ctacgggcag ttgcatcgcg actttactta ttgttacagc aaaggtgtgt tcaagcgtgt
     1981 tctgcctgct gaagagtatg cggaaattcg ctgggatcaa ctggaagcgg gtgacgttaa
     2041 ctttattaaa gatttttact accgtctggc gcatcgcgtg ggtgagttga gtcacctggc
     2101 tgatggttca tatgccatcg cagaacgctg gaatttgggt gaagagtact ggggctacgc
     2161 gaaaaataaa ctctggtcgc cgtttggcta tccggttcac catgccaatg aagcgtcagc
     2221 gcaagtcggt tccattgtta actgtatgtt caaccgtgac tgcatgacgc atacccatat
     2281 caattttatt ggttccggct tgccattgaa actgcagcgt gaagtggcga aagagctttt
     2341 tggttctgaa gatgcttacg atgaaaccaa aaactacaca ccgatcaatg acgcaaaaat
     2401 taaatatgct aagtggtcgc tgttgcgggt ctgtttgcat aacgccgtca ccctgtgcaa
     2461 ctgggtctgg ccaatgaccg tttcgccgct gaaaagccgt aattaccggg gtgatctggc
     2521 gcttgaagcc aaattcttca aagcgatcac tggcgaagat atgactcagg aaaaattaga
     2581 tttagctgca gagcgtatct ttacgttgca tcgcgcctac acggtaaaac tgatgcaaac
     2641 caaagatatg cgtaatgaac acgatcttat ctgttcctgg gtattcgaca aggatccgca
     2701 gatcccggtc tttactgaag gtacagacaa aatggaccgt gacgatatgc atgcttcgct
     2761 cacgatgttc tacaaagaaa tgggctggga cccacagctt ggttgtccga cccgcgaaac
     2821 attgcagcgt ctggggctgg aagatattgc cgccgatctg gcagcacaca atctactgcc
     2881 tgcgtaagga atgggagaga tgaaccatca ggatgaattg ccgttagcga aagtcagcga
     2941 aattgatgag gcgaagcggc aatggttgca ggggatgcgt caccccgttg atacggtaac
     3001 cgaaccagaa ccggctgaaa tcctggcaga gttcattcgc cagcattctg cggctgggca
     3061 gctggtggcg cgcgccgtgt ttctctcgcc accctattcg gttgcagaag aggaactgtc
     3121 agttttgctg gaaaacatta agcagaacgg agactacgcg gatatcgctt gtatgacggg
     3181 aagccaggat gattactatt actcaacgca ggccatgagt gaaaactacg cggcgatgtc
     3241 tttgcaagtt gtggagcagg acatttgtcg cgccattgca cacgccgttc gttttgagtg
     3301 ccagacgtat cctcgtcctt acaaagtcgc catgctgatg caagcgccat attacttcca
     3361 ggaagcgcag attgaagccg cgatcgccgc gatggatgtt gcacctgaat atgccgatat
     3421 acggcaggtg gagtcatcga cggcagtgtt gtacttgttt agcgagcgat ttatgaccta
     3481 tggcaaggcg tatggcttat gcgaatggtt cgaggtagag cagtttcaga acccctgaaa
     3541 tttcaggggg atgtcatgga tagggtggac agttgcagcc actgcattag gggataatca
     3601 gatgtccttc actcgacgca aatttgttct ggggatggga acagtaatct tttttactgg
     3661 ttctgcgtct tctctgttag cgaatacgag gcaagaaaag gaagttcggt atgccatgat
     3721 tcatgacgag tcacggtgca atggctgtaa tatctgcgcc cgcgcatgcc gtaaaactaa
     3781 ccacgtcccc gctcagggaa gccgtttatc gatagcacac attccagtca tcgataacga
     3841 caatggaacg cagtatcact tttttcgtca gtcatgtcag cactgcgaag atgctccttg
     3901 catcgacgtt tgcccgacag gggcgtcgtg gcgtgatgag caggggatcg tgcgggtaga
     3961 aaaatcgcag tgcatcggct gcagttattg cattggcgtt tgcccgtacc aggttcgcta
     4021 ccttaaccct gtcactaaag tggcagataa atgtgatttc tgcgctgaga cccgactggc
     4081 aaaaggcttt ccgcccattt gcgtcagtgc ctgcccggaa cacgcattga tctttggtca
     4141 cgaagatagc ccagaaattc aggcgtggtt gcaggaaaat aaatactatc aatatcagct
     4201 acctggcgcc gggaaaccgc atctgtatcg ccggttcggt caacatttga ttaaaaagga
     4261 aaatgtatga acccgtcgca acatgctgaa cagtttcaga gccagttggc gaactatgtg
     4321 ccacagttca ccccccaatt ttggccggtg tggttgatca ttgccggact attgctggtt
     4381 gggatgtggt tggtgctggg gctacatgcc ttgcttcgtg ctcgtggcgt gaagaaatca
     4441 gccaccgatc atagtgagag gatttatctt tacagcaaag cggtcagatt atggcactgg
     4501 tcgaatgcgt tactctttgt attgttgctg gcaagtgggc tgataaatca ctttgcgctg
     4561 gtgggcgcaa ctgcggttaa aagtctggtt gcggtgcatg aagtttgcgg atttttgtta
     4621 ctggcatgct ggcttggctt tgttctgatc aatgccgttg ggggtaatgg tcaccactat
     4681 cgcattcgtc gtcagggttg gctggatcga gcggcaaaac aaacgcgatt ttatttgttt
     4741 ggcattatgc agggggaaga acatcctttc ccggcaacaa cccagtctaa atttaatccc
     4801 ttacagcagg tcgcctatgt tggtgtcatg tatggattgt tgccattgtt actattgacg
     4861 gggctgctgt gtctctatcc gcaagccgtg ggagatgtgt ttcctggcgt aagatactgg
     4921 ttattgcagg cgcattttgc tctggcattt ataagcctct tttttatctt cggtcatctt
     4981 tatctttgca ccacggggcg tacgccacac gaaaccttta aaagcatggt cgatggctat
     5041 caccggcact aacgaatgat cattacacga gcagacctgc gagaatggcg tatcggagcg
     5101 gtgatgtatc gctggtttct gcgccatttc ccacgtggcg gtagttatgc cgatattcat
     5161 catgcactga ttgaggaagg atatacggac tgggcggaga gtctggttga gtatgcgtgg
     5221 aaaaaatggc tggcagatga aaacttcgcg catcaggaag tttcctctat gcaaaaactg
     5281 gccacagatc ccggagaacg actattttgc agtcagtttg tccgcagcga cgatcatgct
     5341 cgcctcggct gttgtgagga taacgccaga atcgcaaccg ctgggtatgc cgcgcaaatt
     5401 gccagtatgg gttatagcgt gcggattgga agtgtcggct ttaacagtca cattggcagc
     5461 tccggtgcac gcgcacgggt tgctgtaacc ggtaattctt cgcggataag cagcgctgga
     5521 gatagcggtc ggattgcgaa taccggaatg cgagtacgcg tttgtacatt gggtgaacgg
     5581 tgtcatgtcg ccagtaatgg tgacctggtg caaatcgcca gctttggcgc aaacgccaga
     5641 attgccaaca gcggcgacaa tgtacacatc attgccagtg gtgaaaattc aacggtagtg
     5701 agcacgggcg tggtcgactc aattattctc gggccagggg gatgcgccgc actggcatat
     5761 catgacggcg aaagagttcg cttcgccgtc gccattgaag gtgaaaacaa tattcgagcc
     5821 ggagtccgct atcgtcttaa tgaacaacat cagtttgttg agtgctgata gctttacctt
     5881 taatcaaacg aaagacgccg acgcgcacgg gatgcaggtt ttacgaccgc ccgagccatc
     5941 gcctctccaa tttctgcaca tgccgtaatt ccctgaacga aaggctggtc gtgcatcaac
     6001 cagggcaacg cgccgaacgc tacgcgccca cgaatatctt cgggttgctg taacgtataa
     6061 tcttcgccaa cattagggat ttcatcccct gttttctgta attgttcgcg tagtccaggg
     6121 aaaggaatat ctttcacttt aagcggacgc tgcccgcggg catcaataaa aacgtcaaac
     6181 gagtagctat tgtcttccgt tttcaggacg gtgcgcgact cattaatttc catttcgtag
     6241 tcttcaccga gggcgagaat atgaatgatt cccgcttcac gtaaggccag tagtcgacga
     6301 atagactctg aagggattgc cgcataatta tcgatgaaaa cccgggcaag gcctttactg
     6361 aaccgtttat ggtcgtgttc attcagatgt ggaacaattt cctgtacggc ttcatgcagg
     6421 cgcagaatga cataacgcca gggaacagta tgtttttctc gtttattgcg ttcgacttcc
     6481 tggagatttt cttctgccca gtcaaatggg tcgcgttgtt tgcgctcggc aaaccaggct
     6541 tgagcaaagg aatcgacatt caggctctct aaggctatgc gttggctcca gtctggatca
     6601 gcaaacttga tttcctctac tatcaatcta aatacccgat ccaaaaggcc cacttcgcct
     6661 ttttgaatct cagcatttaa tgcctgatcg gtgatgatgt gtaagggctc gtagggaata
     6721 gggcaataga aatcggcttc gggtaaaata cctgtgcgcg acattaacgt aatatttagc
     6781 ttttcatttg cgttatcgcg gtgaaaagtg acgtgttgtt tatcatcttc aatgaacgaa
     6841 ccatgctgaa tagccactgc cattgccgca tccagtccgc tcaaggatgt tcccataata
     6901 cccacgttac acgcatcgac ctttgcttcc atcaggcctg accacgggct gggaaaatac
     6961 gttctggttg cttcttcttc atcaggccag acgtgccccg ttgcgatcac cgctaaatca
     7021 aacgtctcgc tgggtaaatt cagatttgta gcgagcatga cgccagcatt tgtaatttgc
     7081 agatcggtaa cctggcatga ttcataaaca gccactgcaa atttttgctg tcgtgcctgg
     7141 tctactaatc gtaaaaattg atcgcggaaa tattcgccca gcagaattcg cggtaaaaac
     7201 tgacgatcgt gcaaggtttc ttttttaacg ccataacgct ggagatggct ggcttcttgc
     7261 ttttgtagcc attcgagata cgtgcaataa atcggcggta tttcaatact ggcaatgttt
     7321 gccagcatca gttttgagtt ttcctcatca ctgtatggca tcccgatacc ggcttcgtca
     7381 gcctgatcga agatagaaat agaaagtggc gtttgttgct gtagaagcga gaataaggtg
     7441 tagatccccg taggcccggc acccacaata gcaattgttt tcattgttac ttacctgtac
     7501 gcattctttc gaatttagtg ttatcaggta agtttggttg agatttttag aatttgagaa
     7561 atttcagaca gggatgaaaa gggcactaac cgcccttgat taaaatctgt caggcaagtt
     7621 ttgcctgatt gatttgactc agtggcacat tgacatcgaa aactttggcg acaacttcct
     7681 caacgccgag atttttcagt cgggcagtat gtttttccag ataggcaagg gcgctttttt
     7741 catcagtgaa aaggtagatc ccaccggctt cgtggttctt ttcactttct gtccaaacct
     7801 tccacagaaa accaggttcc tgattaatcg actcagcaag cggtttaagc tgctcagcca
     7861 ttgtgtcgcc aaatgggcca ttgaaagaaa aatgaagttg taacaaagtt gccatagcga
     7921 ttctccttct gataacgcat acgaagacag tgtaaatctt ttaacgtatt ggatacagag
     7981 aagaaacaga gggaaaaaag gagaagacca gattaagaaa atctggtgcg tccgagtgga
     8041 ctcgaaccac cgacccccac catgtcaagg tggtgctcta accaactgag ctacggacgc
     8101 aggatggtgc gttcaattgg actcgaacca acgaccccca ccatgtcaag gtggtgctct
     8161 aaccaactga gctatgaacg caacgttgta ggtgacaacg gggacgaata ttagcggcag
     8221 agtgggaagg tggcaagagg caaaacgtaa ttttctgcgc tatttcgacc gtttgcagag
     8281 cttttaagca aattggctat attttgttga tttgcaaggg cgatttttat tcaggatcgc
     8341 atttacatct gatacaaccc aataatccgt gtatcagagt ttaaacagag ttgtttgcca
     8401 ccatctcaaa tctgtcgtgg ataaatcctt cgtagccttt acacttaggt agcaataaag
     8461 gcacacagat gcagcaaagg aataaatcat gggatctgat gcgaaaaact tgatgagcga
     8521 cgggaacgtg caaattgtta agaccggcga ggttattggc gcgacgcaac ttactgaagg
     8581 cgagttgatt gttgaagctg gcggaagagc cgaaaatacc gtggtcacgg gggctggctg
     8641 gttgaaagtg gcaaccggtg ggatagccaa atgcacacag tacggtaaca atggcacact
     8701 atcggtcagc gacggtgcca ttgccacaga tattgttcag tccgagggag gcgcaattag
     8761 tctctctacg ctcgctacgg ttaatggccg ccatcccgaa ggtgaattca gcgttgataa
     8821 aggttatgcc tgcggtttgt tgctggaaaa tggcggtaac ctgcgtgtac tggaaggcca
     8881 tcgcgcggaa aaaattattc tcgatcaaga gggtggcctg ttggtcaatg ggacaacctc
     8941 agcggtcgtg gtagatgaag gtggtgaatt gttggtgtat ccaggtgggg aagcctgcaa
     9001 ttgtgagatt aatcagggcg gcgtttttat gctggcgggg aaagccagtg atacgttgct
     9061 tgctggtggc accatgaata atctcggtgg tgaagactct gacactattg ttgagaatgg
     9121 agccatctat cgtctgggga cggatggtct tcagctctac agttccggta agacgcaaaa
     9181 cctgtccgtt aatgtgggtg gtcgggctga agtgcatgcc ggtacgctgg aaaatgcggt
     9241 aatacaaggt gggacagtga tcctgttgtc acccaccagc gcggacgaaa attttgtcgt
     9301 agaggaagat cgcgcaccgg ttgaactgac cggtagtgtt gcattactgg acggcgcttc
     9361 aatgattatt ggctatggcg cagatctgca acaatcaacg attactgtac agcagggcgg
     9421 tgtattgatt ctcgacggca gtacggtaaa aggtgacagt gtcacttttg gtattggtaa
     9481 catcaatcta aatggcggaa aactgtggct gatcactgat gcggcaacgc atgtgcaact
     9541 gaaagtgaaa cgcctgcgcg gagagggagc catttgcctg caaaccagtg cgaaagaaat
     9601 ttcacctgac ttcatcaatg tgaaagggga agttactggt gatatacacg ttgagataac
     9661 agatgccagt cggcaaactc tgtgtaacgc actgaaacta cagccagacg aagacgggat
     9721 tggcgcaacg ctccagcctg cgtaaaaaca caggcgcaac ccgtgaaggt tgcgccttgt
     9781 ctttactagc gagctgctcg ttgcagaatg atggctgacg gcagacgttg caggaaccgc
     9841 atacgcagca tcatcataat ggctgccgaa gtcaggccaa taataaagcc tatccagaag
     9901 cctgctggcc ccataggttc aacgaccaga tcggtcagtg ccagaatata gccgcttggc
     9961 aagcccagca cccagtaagc cgtaaaggtg atatagaaaa tggaacgtgt atctttataa
    10021 ccacgcaaaa tcccactgcc aatcacctgg atagagtcag aaatctgata taccgccgcc
    10081 agcagcatca aatgcgcagc cagcgttaca acctcgggat tgtcgttgta caacagggcg
    10141 atttgctctc gcagtgaaac cgtgaatatg gccgtcaggg ttgccataca gacacccacc
    10201 ataagcccgg tccgcgcagc ggtttgcgca tccagcgttg agccttgacc cagacgataa
    10261 cctacgcgga tggttaccgc tgccgccagc gacatgggaa gcacgaacat cagtgaacta
    10321 aagttcaggg caatctgatg tcctgcgaca tcaacaatac cgagcggaga cactaacaga
    10381 gccacgacgg caaacagcgt tacttcaaag aacagtgcca gcgcaattgg caaaccgagt
    10441 tgaatcagtc gtttcataac cgcaggatcg ggcttcgcgg tgcctttttc gttacgaata
    10501 tcgcgcatcg agcgggcgcg tttaatgtaa gaaaccatgg caaggaacat gacccaatac
    10561 accgccgcag tagccacgcc acaaccaacg ccaccgagct caggcatacc gaaatgacca
    10621 taaataaaga tatagttcac cggaatgttc accagcaggc cgataaagcc catcaccata
    10681 cccggcttgg tttttgccag accttcacac tggttacggg caacctggaa gaaaagatag
    10741 cccggcgcgc cccatagcaa cgcacgcaga taacccacgg ctttgtccgc cagagccgga
    10801 tcgatgtttt ccatggagcg gataatgtaa cccgcattcc acagtaccag cataatgaga
    10861 acggaaacaa aacctgccag ccagaaacct tgtcgcacct gatgcgcaat gcgctcacgt
    10921 cgaccggaac catttaattg cgcgataacc ggcgttaatg ccagcagcag tccgtgacca
    10981 aagaggatcg ccggaagcca gatagaagtt ccgatagcaa ccgccgccat gtcggtggca
    11041 ctatagccgc ccgccatcac ggtatcgaca aaacccatcg cagtttgggc gatttgcgcg
    11101 agaatcaccg ggattgctaa tgctaataac agacgcgctt cactgatata cttctgcacg
    11161 tgaacacctt ttatttgtag ttatatgaga gactaaaaag ccgcctgaat gggcagcaaa
    11221 aagaagaagc aggggaaatt cagtctattg tagcgaggat ttattaattc tccagtgaaa
    11281 aaatagccat tcttacccct tcactggtaa gggcgttttc cacctgctat tgtgctgaac
    11341 agaatgtctt aactgatttc aggagttgta agtatgttta cggggattgt acagggcacc
    11401 gcaaaactgg tgtcgattga tgagaaacca aattttcgca cgcatgtggt ggagttaccc
    11461 gaccacatgc tggacggact ggaaaccggt gcgtccgtgg cgcataacgg ttgctgcctg
    11521 accgtgacgg aaattaacgg caaccatgtc agttttgacc tgatgaaaga aacgttacgc
    11581 attactaatc ttggtgattt aaaagtgggg gattgggtaa acgttgagcg tgcggcgaaa
    11641 ttcagcgatg aaattggcgg acacttaatg tcaggtcata ttatgaccac cgctgaagtg
    11701 gcgaaaatat taacctcgga aaataatcgt cagatctggt ttaaagtcca ggatagtcag
    11761 ttgatgaaat atatcctgta caaaggattt attggcatcg acggtattag cctgaccgtc
    11821 ggcgaagtca cgccaacgcg tttttgcgtc catttaattc cggaaacact ggaacgcacg
    11881 actcttggga agaaaaaact cggcgcacgc gtcaacattg aaatcgatcc acaaactcag
    11941 gcagtggtag atacggtaga acgtgtgctg gcggcacgag aaaatgccat gaatcaacca
    12001 ggtacagaag cctgatctga aatcgccccc ggagcggggg cgatagaata gcctttagcg
    12061 agcgactcga aggccgtttt caacaccgcg tgagaacacg acctgccaga gctgaatatc
    12121 acgggcgcgg aaagcacctg cacaggcatt cagataatag gtaaacatcc gtttaaagcg
    12181 ttcactgtag ttatccgcaa tttctggcca tgcggcgagg aatcgttcat accacgccat
    12241 caacgtagta tcgtaatcag caccgaagtt atgccagtct tccatcacaa agtggggttc
    12301 gctggactga gctatctggc gtacagaggg caggcaaccg ttcggaaaaa tatatttatt
    12361 aatccaggga tcaacattca gatcggtttt tttcgaaccg atggtatgga gcaggaatat
    12421 accttccggt ttcaaattac gatccaccac cgcaaaatag gtatcgtaat ttttcggtcc
    12481 gacgtgttcg aacatcccca cagaaacaat acgatcaaac tggtcgttca ggtcacgata
    12541 atcttgcagc aaaatggtga catccaggcc ttcacagcgt tcctgagcca ttttttgctg
    12601 ttcggcagaa atggtgacgc cgaccacgct tacgtcataa ttagacgcca tgtagtgcgc
    12661 cagtccgccc cagccacagc caatatccag tacgcgcatc cctggtttta actgcaattt
    12721 ttcacaaatc attttgagct tcgcctgctg ggcagattcc agattatcgg catctttcca
    12781 gtaagcacag gaatattgca tgaaggggtc aagcatgcgg ctgaacaagt cattacccaa
    12841 atcgtaatgc tctttgccga ctatccaggc acgtttttta ctctgcagat tgaagagacg
    12901 agcgccggca atacgcagcg tgtctttgaa atgatgggga agttggttct cgagacctgc
    12961 gcgtaagact ttgctaaaaa acatatccag tcggtcacat tcccaccagc catccatata
    13021 actttcgcct aaccccaacg atccttcctg cagaacgcgt ttaaaaaaat cggggttttt
    13081 gacacgaata tccgccgggg cagaaccgtt aatggctata ccggcacggc taagtaattc
    13141 gttggcgata cggtaccagt tgtcatctgg tacactgact tcttctatac acgatgaact
    13201 catagtttct ccatcactgg tcggtgatca gaaccttaaa acagcgtaga cgctttttgg
    13261 gctttgtgag aaatctcgca gaaaaaaccg cgagtctata atccgacgta gaacagaggg
    13321 aaaggaggaa cccttgccga aatgaccatc catagtgagt cgggaacgat cctgtccccg
    13381 taataacata atatttatcg tagttttaac aactcgaaat tagtataggc cgctaatttc
    13441 aatcatacaa caaaattttg ttagcagcct aagtataagt atatcgatat agatcagtgt
    13501 gattcactat gagcgacttc ggcattgcca tgattctggc agccaacttc ttcacaacgt
    13561 tgcatcatgt aacccagagc gaccagcact actgttgata acatcacgct ggtggtggtg
    13621 agcaatggcg tactgatact gataagccag gaaactacca gacttgcgag gaagcacaga
    13681 cccagttgca gagtgttctg caacgctgcg gcgcgaccgg ttgcatgtgg gaagggacgc
    13741 agcgcctggg caacaacaat cgggtagatc gcgccgttgg caatcgccat cacacagaat
    13801 gggatcagga tttcgaccaa cgacacatgg ctaatgaagc ccgcagccca ggtcgcaatg
    13861 acgctgacag caaacagtac cagcaaccac ggtaataact gcttgccttg ccatttctgc
    13921 agcgcggcgc gacagccata accaccaatc agaaacgcga tagtttgcgg gacataactt
    13981 aaaccaataa ctgccgggct gtaacccatt tcactaagga tgaacggtga accggtcagc
    14041 catgcaaaaa aactggctga acaggctgcg tatatcagca cgttgccgcg ataggtttta
    14101 gaacgtagca ggtcggtaaa ggtcagacca tcctgactat tgttacgggc cttcgtcgtg
    14161 ggtttgagcc agaaaatagg cagaatcagc accacggtaa tggcaaacag ggtggcgaaa
    14221 atcgcctgcc aggaaaaatg gaccagcagc cagcttccta acagaggagc cagtgccgga
    14281 gatagaccca ccagcggcat gatggtcgca aaaatacggt taactttctg tgaaggataa
    14341 taatctgtca ctaatgcttg ccagataacc gccgcggcgc agacacccac agcctgtaca
    14401 aaacgcaata tcagcagcgt ggcggcgttt tccacccaca gcatccccag actacctaac
    14461 gcaaaaattg tcaggccgat gaataacacc ggtttgcgac cataacggtc ggagagtggc
    14521 ccccacagaa gctgggctgc ggcaaaaccg gcaaggaaca gactaaggct ggcactgaca
    14581 gcagacgcag gcgtttgcag gtcggcctgt atggcggcga aagcaggcag atacatatcg
    14641 gttgccagaa aaccgagtac gctcaaaccc gccagccaga ctaaaaatct tttcccaggt
    14701 tgcatgtata ttctctaaat ctctcaatta aacaccgccg caaagtgtag ggagtgaaat
    14761 tagtgttgtg aaacggtaat atttgatgat tggtttcaaa aattttgcag gcagactatg
    14821 tggtcagaat attctctcga agttgttgat gcggtagcgc gtaatggtag ttttagcgcc
    14881 gcagcccagg agttgcatcg cgttccttct gcggtcagct ataccgtgcg tcagctggaa
    14941 gagtggctgg cggtgccgct gtttgaacga cgtcaccgtg atgtggaact gaccgcagcc
    15001 ggggcgtggt ttctcaaaga agggcgctct gttgtcaaaa aaatgcagat cacccgccag
    15061 caatgtcagc agatagcgaa cggctggcgc ggtcagttag ctatcgcagt ggataatatt
    15121 gtcaggccag agagaacacg gcagatgatc gttgattttt atcgccattt tgatgatgtc
    15181 gaactccttg tctttcagga agtgttcaac ggtgtctggg atgcactttc cgacgggcgc
    15241 gtggaactgg cgattggcgc aacacgcgcg attccggtag gcggtcgtta tgccttccgg
    15301 gatatgggga tgttaagctg gagttgcgtt gttgccagcc accacccgct ggcgttgatg
    15361 gatggcccgt tcagcgatga tacgttgcgc aactggccgt cattggtgcg cgaagacacc
    15421 tcgcgaacgc tacctaaacg tattacctgg ttgctggata accaaaaaag acttgtcgtg
    15481 cctgactggg aatcatcggc aacctgtatc tcggcaggat tatgcatagg gatggtgcca
    15541 acacatttcg ccaaaccgtg gcttaatgag gggaagtggg tagcactaaa actggaaaac
    15601 cccttcccgg attcggcatg ttgtctgaca tggcagcaaa atgatatgtc gcctgcgtta
    15661 acctggttgc ttgagtatct cggcgatagt gagacgctga ataaggagtg gctgcgggag
    15721 ccggaagaga ctcccgcaac gggtgattaa cgacgatagt cgcggaacgg gccgtcagcc
    15781 acggagcggc gttcaatcaa ccgcggatgc acttcaatgg actgcggttc ttcacgtttg
    15841 ttgacgatac gatccaacag catgttgaac gctgtttcgc ccagcgaatc ttttggctga
    15901 tggatcgtgg tcagcgccgg cgtaaaatag cgggcgttgc gcacgttatc ataaccgatc
    15961 agcgaaacat cctgcggcac gcgcagaccc atttcatcag cagcacaaag tgcgcccatt
    16021 gccatgatat cgccaccaca gaagacggca gtagggcgat gcgactgcga caggatttgc
    16081 tgcatggcgc gataaccgga ttcaggttca aagtcaccct gcacaatcca gctttccggc
    16141 actttgatca tcgcttcttc catcgccttc ataaaaccag caagacggcc tgcgccggtg
    16201 ttacgttcca gcgggccagg gataacgccg atttcgcgat gaccgcgttc aatcagataa
    16261 cgcccggcca tgtagccgcc ttcgaacgcg ttatcaatga ccgcatcggt gaagtcagct
    16321 tttgcttcac cccagtccat caccaccatt gggatatggc gatactcttc cagcatcgcc
    16381 agcaacggct ctgggtactc agaacacatc accagcagac catcgacgcg tttttgcgcc
    16441 atcatcgaca gataagcccg ctgtttctca agattgttcc acgcattgcc cagaatcagg
    16501 gtgtaacctt tctggaagca atttttttcg accgcttcaa tgatctcggc aaaataggcc
    16561 gcttcgctgc tggtcgccag caaaccgata gacttggtgt ggttaacctt caggctacgc
    16621 gccaccgcgc taggggagta gtgtaattct ttgattgctg cccacacggc attgcgcgtt
    16681 tcttcagcga cgaaacgtgt tttgttgatc acgtgtgaca cagttgtagt ggaaacgttt
    16741 gctcgtttcg ctacatcttt tattgttgcc attccatttc actccagacc ctaacttcag
    16801 ctcctgcaaa ttcgcaaggt aaacgtttgc ctttacacac cttacgcaca acaaaaatgt
    16861 tgcggtacgc cggaaaaacg acacggagcg tcaggaaggg gtcaatggcc agtacgcaaa
    16921 tagtgtaagc gtggaatttt ggccgatctt gacgaaaagg ggaagtagta aaaacactta
    16981 tcattataaa tgacgggaga ttttttcatc acagtgtgta aaaatgcgta atatcacttt
    17041 ttgtgtggta ataaaaaagg agaaaacttg atgagcaccg accttaaatt ttcactggta
    17101 acaacgatta tcgtcctcgg tttgatcgtg gccgtgggtt tgactgccgc gctgcactga
    17161 tttcctctcg agggagcgcg ttctccctcg catctccttc ctgtttgtta gcaaaatggc
    17221 aaaagtgttt tctctttttg ttattttcat ttttttgtga ttactgtcat gctttcgcca
    17281 ctttaatgaa tatcgcgtaa tgacgcgaag aggtggagat aaggcatgaa aattaactat
    17341 ccgttgctgg cgctggcgat tggcgcgttt ggtatcggga caacggagtt ctcgccaatg
    17401 ggcttgttgc ccgtcattgc gcgcggtgtg gatgtctcga ttcccgctgc cggaatgtta
    17461 atcagtgctt atgcagttgg cgtaatggtt ggcgcgccgc tgatgacgct tctactttct
    17521 catcgtgccc gccgcagtgc gttgattttc ctgatggcga ttttcacgct tggcaacgta
    17581 ctttccgcca tcgcgccgga ttatatgacc ctgatgcttt cacgcatttt gaccagcctg
    17641 aatcacgggg cattttttgg tttgggttca gtcgtggccg caagcgtagt gccaaaacat
    17701 aaacaggcca gcgcagttgc cactatgttt atggggttaa ccctggcaaa tatcggtggc
    17761 gtgccggcgg cgacctggtt gggcgaaacc atcggctggc ggatgtcatt tctggcaaca
    17821 gcggggctgg gcgtgatttc aatggtaagt ctgttcctct cattacctaa aggtggcgca
    17881 ggggcgcgac ctgaagtgaa aaaagagctg gcggtattaa tgcgtccgca ggtgctgtct
    17941 gcattgctga cgacggtact gggcgctggt gcaatgttta ccctctacac ctatatctct
    18001 ccggtactgc aaagcattac ccacgtaacg ccggtgttcg tcacggcaat gctggtgctg
    18061 attggtgtcg gattctctat cggtaactat ctcggcggca aactggcaga tcgttcagtt
    18121 aacggcacgt tgaaaggctt tttgttgttg ctgatggtga ttatgctggc aatcccgttc
    18181 ctggcccgca atgagttcgg cgcagctatt agcatggtgg tgtggggagc tgcaaccttt
    18241 gcgatcgtac cgccgttaca gatgcgcgtg atgcgtgtcg ccagtgaagc gccaggtctg
    18301 tcttcatcag tcaatattgg tgcctttaat cttggaaatg cgctgggagc agctgctggt
    18361 ggtgcggtaa tttccgctgg gctgggatac agttttgtgc cggtgatggg ggcgattgtc
    18421 gcgggactgg cattattgct ggtgtttatg tcagccagaa aacaacctga agcagtttgc
    18481 gttgccaaca gctaattaaa caaaaacgca gaaggataac cttctgcgtt acagttgctg
    18541 gcaacgtgcg atttatcttg ccgaatgcgc tgttaacgcc tcatccgcgt tggcatggta
    18601 agcgaagcgc atcaggtaat gttgcatttg ccatcagtta ttatgcagcg agatttttcg
    18661 ctacgaattc ccagttcacc agcgcccaga agtgctccag atagccaggg cgtgcattgc
    18721 gatagtcgat gtaataagcg tgttcccaga catcaacggt cagcagcgga gtcgcatcgg
    18781 tggtcagtgg agtacccgcg ttagaggttg aaacgatagc cagtttgcca tcgctgtttt
    18841 tcaccagcca ggtccagcca gaaccaaagt ttttgatcgc tgcatcagta aactgagctt
    18901 tgaaatcggc aaagctgcca aaagatgcgg cgatagcttc agcgactttt ccagtcggtt
    18961 cgccacccgc gttcggtgcc aggcagttcc agtagaaagt atggttccag acctgagctg
    19021 cgttgttgaa tacgccacct tcagagctgc gaataatctc ttccagtggt ttaccttcaa
    19081 acgcggtacc tttgatcagg ttgttcaggt tagtgacata agtctgatga tgtttgccgt
    19141 agtgatactc gatggtttcc gcagaaatgt gcggtgccag agcatcttta gcatatggta
    19201 gtgcaggtaa ttcgaatgac attgctactc tcctttatta ttaatttgca tacgtacaat
    19261 agccttattg tgcgtatgga tagggtagca aattaaaagg tgagacaaaa gatgaactta
    19321 ccctgttgcc gtaacaacag ggtaaagttt tagcgaagtg tttttggggt cattacccgg
    19381 cgagcgccaa catagtggcg ctgccagtag tcttcactga gagaagtgat ttgaatttcc
    19441 tggcccgtgc gcggtgactg aataaatttg ccgttgccga catacacgcc gacatgatcg
    19501 gctgtaccgc gtccctgagt acggaagaag accaggtcgc cgtttttcag ttcactacgt
    19561 tcgattggcg ctgcatcgcg caggtgatac atttcattcg ccgtacgcgg aatacgaatt
    19621 ttcaccaaat ctttataagc gtaataaacc agaccgctgc aatcaaaacc ggtacgcggt
    19681 gagctgccac cccaacgata tggcttacca atttgctgca tcagtttatt cattgccact
    19741 tttgtggctt tttgcacctt cgctttgtgc gcgtcggcaa gggttcctga cgcggcattt
    19801 ttgactttca cacaatgcga tttatagccc ttacgtttgg tacacttttc ggtaaagctg
    19861 gctgaggccg ttttattgac ggcgtgtttg ctgcgagttt tggcgttttt agaagcggta
    19921 atggaagatt ttttaacgga agaagcggtt ttgcttttgg tcgtactttt actggcggct
    19981 tttttcgctg ttttctggct ggctttctgg gtcttttggg tggttgccgt gcttttcttt
    20041 ttatctgctt ttttggtgat atgagtggta gcagaactct ccctggcttg ctttgaagca
    20101 tgggccatag gcgttaaagg cagggtggta aaaagtaaag cacagagcgt gatcgaaata
    20161 cggtttatcc gcgccactga gcgtccctct gttaaggcag gtctatcatg cctgcgtctg
    20221 atttacaaaa cttgatgatt ctaatgcata gaagaagaga aagttaatag cttttattgt
    20281 taaaaaatac gtttcgtcgg cgagcgcaaa aaaatgcagc agtgaaagct aaaaatggtt
    20341 ataaatacgc cagtttgccg tgcatcaggg aagaaacgaa tatttgaaat gcaacaaata
    20401 gagcaaaggg ataaaatagc aaaagcgtga tgaaaatgtt attttccgat gccgcactga
    20461 cccgctacaa tgtcagatta ctgccgtaaa gaagtttgag gaagcaagac aatgagcacc
    20521 actatcgaaa aaatccaacg ccagattgct gaaaacccga tcctgctgta catgaaaggt
    20581 tcaccgaaac tgccgagctg cggtttctct gcccaggcag tccaggcgct tgccgcatgt
    20641 ggcgaacgtt ttgcctatgt tgatattctg cagaatccgg acattcgtgc ggaactgccg
    20701 aaatatgcga actggccgac cttcccgcaa ctgtgggttg acggcgagct ggtgggcggt
    20761 tgtgatatcg tgatcgaaat gtatcagcgt ggtgaactgc agcagctgat caaagaaact
    20821 gccgctaaat acaagtctga agagccggac gcggaataat ccgtcctgca atactgacgg
    20881 atgcgaacgg gcatccgtca gtcctttctc tatccccaat ccagcccttg tggcgaactt
    20941 gaaaatcccg cctcacgtaa cagcgtaaac atcggcgttt gccggaccgg taggtcattc
    21001 acttctgtta gcgtaaagcg cagccgtggt tcgcgactag acagcggtcg gctgtttaaa
    21061 ggcgcgaaga aaccagtcgc gggtcgccgg cgaaaacacg tccggcaggg gcgatgaagg
    21121 gtttggatta tttgccatta acgctattcc ccgtgaatat catgcatgat gcgtggaata
    21181 gcctcactat agatgcctga atcattgcat gtcatcaggc atcgactcga ttacacctct
    21241 gcggcggcag gtagtggcca gcctcccaga cgtttccagc ggttgacgat ttcacaaaac
    21301 agcacagcag tgcgttcggt gtcgtacagc gcggagtgcg cctgggtgct gtcgaagtcc
    21361 atgccagctg tctggcaagc ctttgacaat acggtttgtc cgagtgccag cccggccagt
    21421 gcagcagtgt caaaagtggc gaaagggtgg aacgggttac gtttcagtga ggcgcgttct
    21481 gcggcggcca tcataaagct gtgatcgaaa ttggcgttgt gcgccaccat aatggcgcgg
    21541 ttacagccgc tcgctttaat acctttacgt acaactttaa aaatttcgtg cagcgcctcg
    21601 tactcgctga ccgcgccgcg atcgggatcg ttcggatcaa tgccattgaa ggcaagggcc
    21661 tctggttgca aatttgcgcc gacaaatggt tcgacgtgga aatgtaatgt ggtgtccggc
    21721 atcagccagc cttgttcatc cattttcagg gtgatggcgg caatctcaag cagcgcatcg
    21781 gttttggcgt taaatccggc tgtttcaaca tcgatcacaa caggataaaa accacgaaaa
    21841 cggtcgcaca gaccggtaag ttgagcgtta tcggacatct gattctctta atacggataa
    21901 attgcagcgc gcattatgac aaatattact tgtcgatgca gtaaagatgc gggcgcgatg
    21961 agttcacgcc cggcaggaga ttagttgccc agaccgcgac cggcgtcttt ctcttcgatt
    22021 aattcaattt tgtaaccgtc cggatcttcc acaaatgcga taaccgtagt accgcctttt
    22081 accggacccg cttcacgggt tacgttaccc ccgttttgac ggattttttc gcacgcttca
    22141 gcggcgttat ctacgctaag cgcgatgtga ccataagcag tgccgagttc gtatttatcc
    22201 acgccccagt tgtaggtcag ttcaatcact gcttcttcgg tttccgggcc gtaaccaaca
    22261 aacgccagtg agtatttgta ttccgggttt tcgctggtac gcagcagttt catgcccagc
    22321 acgttggtat aaaaatcgat ggagcgttgc agatcgccaa cgcgcagcat ggtatgaaga
    22381 agacgcatct ttatatcctc aaaatggcta acagattcaa aaagttgttt tagtatagcg
    22441 gcggctttgc gccgctcgca atgttggatt acagcgtcgg gtaatcagtg taaccttcag
    22501 caccgccacc ataaaaactt tcggcacgct gcgggttaag ctcagctttg cgctgcaagc
    22561 gggcgaccag atccgggttc gcaatccagt cacgaccaaa tgctaccgca tcaattaacc
    22621 ctttgccgat cagcgtttca gctttttcta ctgtgtatgc acctgcgccg ataatcggac
    22681 cgtggaaacg ggcgcgtact ttttcgcgaa acgcatcagt atatggttca cctcccgccc
    22741 aatctggttc tgacatatga agataagcaa tgccgcgttt acccagttgt tcaatcagat
    22801 acagtgcatc ggcttcttca ttcgggccgt tatccgtgtt ctggaaagta ccgattggcg
    22861 aaatgcgaat gccaatgcga tcggcacccc attcttcaat cccggcatcg accacttcca
    22921 gcaccagacg cgcgcgattt tccacgctgc cgccgtactg atcggtacga tggtttgaag
    22981 aaggggaaag gaactgatgc agcaaataac cgtgagcaga gtggagctct accagatcaa
    23041 aaccggcttc acgcgcgtta gcaatggcct gacggaaatc attgacgata cccggaatct
    23101 cttccagttc aagcgcacgc ggcatggatg tttcaacacg gatcgcctga ccattttcat
    23161 cgcgcagaga agtacgtgtt cccgcgctaa gtgctgaagg cgctaccggt gcctgaccgc
    23221 caggttgcag gctggcgtga gaaatgcgtc cggtgtgcca cagctgtacg gccatatgac
    23281 cattttcagc atgaacgcca gcggtaattt ttttccatgc ggcaatttgt tccgggctat
    23341 ggatgccagg tgcacctgcg tatccttttg cctgggcaga aatttgcgtg gcttcgctaa
    23401 taatcaaacc ggcactggca cgttggcgat agtattccgc catcaacggg gtagggatgt
    23461 cacccggttc aatactgcgc agacgcgtca gcggtgccat aaaaatacgg tttgccgccg
    23521 tgatcgcgcc cactttcagt ggggaataca gtttttcaga tgacataaag gctcccggtc
    23581 gtctggtgat aaaaaatgcc tgctaaacgg caggcgtcgc aataatgttt tttacatggg
    23641 ccagcgcgtt ttccagtggc tcgaaactgc gcgaaatttt ggcctgcaaa ttcgcaccaa
    23701 gccacagtgc gtaaagcact tgtgcctgtt gcagcggttc gccacaaaag gttaaacaat
    23761 ggttctcacg gccattttcc agcgcctgag agagcagggc gatcacgccg cgagcccctt
    23821 tatccattgc gctgcgcata tcttctgaca gatcgcacac ttcggcagag agttttactg
    23881 tcaggcaacc actgatggtt ccatgttggc aaaactggtt cagtgtttgc tggtaataag
    23941 ccagtatgcg gtcgcggtag ttaccttcgc cggattgcag caactcagtc agtcgctggt
    24001 gatatgcggc gtaatggcgc tcaagcatgg caacgccaaa cgcttcttta gaacgaaagt
    24061 agtgatagaa ggaccctttc ggcacttcag cggtttttag gagttcgctt agccccatcc
    24121 cggtgaatcc acgttgcagg caaagttgct cgcccgtcgc caggagatgt tcgcgagtat
    24181 catgttcggt gtgtttgttc atgcgttgga gtgtagtaga ccagtcggtc tagtgcaagc
    24241 aaagttgcta ctttggacat taacagcttc aataatggta ataatcttat ctgaggaggg
    24301 cgaggtgacg gagcaattag agttctttcc cgtccagagc ccgtgccggg gaatttgcca
    24361 gtctgacgaa cggggatttt gtcgcggctg ttttcgtagc cgggatgagc gttttaactg
    24421 gaataaaatg agcgatggcg aaaaacaaga agtgttgcgc ctttgtcggc agcgattaat
    24481 gcgcaaatta cgtgcaaata agccagcgtc atcagacgaa ccggagcaac catcactctt
    24541 ttgagcctaa tcttgcgtat actcctctca tatctgataa gaggaagcga ttatggttca
    24601 gcgtattact attgcgccgc aaggcccgga gttttcccat tttgtgatgg gctactggcg
    24661 attgatggac tggaacatgt ccgcccgcca gttggtcagt tttattgaag agcatctgga
    24721 tctcggcgtg accaccgtgg accatgctga tatttatggt ggctatcagt gcgaagcggc
    24781 gtttggcgag gcgctgaaac tggcacctca cctgcgtgaa cggatggaaa tcgtcagtaa
    24841 atgcggtatc gcgacgaccg cgtgtgaaga aaacgtcatt ggccattaca tcactgaccg
    24901 cgatcacatc attaagagcg ccgaacagtc gctaattaat ctcgcgaccg atcatctgga
    24961 tctgctgtta atccaccgac cagacccgtt aatggatgcc gatgaagtgg cggacgcctt
    25021 taaacatctg catcagagcg gcaaagtgcg tcattttggt gtatcgaact ttacacctgc
    25081 gcaatttgcg ctgttgcaat cacgcctgcc atttaccctt gccactaatc aggtggaaat
    25141 atccccggtg catcagccgt tactgctgga tggcacgctc gaccaactgc aacaactgcg
    25201 tgttcgtccg atggcgtggt cctgccttgg tggtggtcgt ctgtttaatg atgattattt
    25261 ccagccgctg cgtgatgaac tggctgtggt ggcagaggag ttaaacgcgg gctcgattga
    25321 acaggtggtt tacgcctggg tattacgttt accgtcgcag ccgctgccaa ttatcggctc
    25381 aggtaaaatt gagcgcgtac gggcagcagt cgaagcagaa acactgaaaa tgacccgtca
    25441 acaatggttc cgtatccgta aagcggcact aggttacgac gtaccgtaag cacttttagg
    25501 aatagccgcc gttcaaaaat gtgtcactgg tttacactta ttcaggaatg cacaatgaac
    25561 ggaggtccta tgaaacgttt tagtctggcc attctggcgc tggttgttgc gaccggcgca
    25621 caagctgcca gtgaaaaagt cgagatgaac ctcgtcacgt cgcaaggggt tgggcagtca
    25681 attggtagcg tcaccattac tgaaaccgat aaaggtctgg agttttcgcc cgatctgaaa
    25741 gcattacctc ccggtgaaca tggcttccat attcatgcca aaggaagctg ccagccagcc
    25801 accaaagatg gcaaagccag cgccgcagaa tccgcaggcg ggcatcttga tccacaaaat
    25861 accggtaaac atgaagggcc agaaggtgcc gggcatttag gcgatctgcc tgcactggta
    25921 gtcaataatg acggcaaggc gaccgatgcc gtcatcgcgc ctcgtctgaa atcactggat
    25981 gaagtcaaag acaaagcgct gatagtccac gttggcggcg ataatatgtc cgatcaaccc
    26041 aaaccgctgg gcggtggcgg tgaacgctat gcctgtggtg taattaagta attaagaggc
    26101 cagcgtacct tgcggcggtg cttgctcaag ttgcgaaagc gagcagtaca gccgccagac
    26161 aattgccgcc agttctcggg cggcaggttg atgatgacgg gcaaggctgt cgcaaatccg
    26221 ctgtaattct tcaagtgtgg ccgccagtga tttttgctga acgccacgct cactcatcac
    26281 tccccgcaac agtgaaatac agttatcccg tactcgcgat aacggatcgg aacgcgattc
    26341 ccagtcgcgc aattgccaga caacatgaga acagttcagc aacactacac cccagcgtaa
    26401 taaccaacgg cgagccagcg catcctggct gttactcaac tgactgacgt gatgataagt
    26461 gagcgattca aattcgcttt cactcagtgt tggatggcgg cttagttgat cgacaaaatc
    26521 ccggcgcagc gcgcgaatat ggcggcggct tttacgagca tccgatcctg gacgcagaat
    26581 ggcgaacgct aaccacgcca acgcgacgcc aacaattttt gccagattat cgttaagaaa
    26641 atcagcaaaa tcatacaccg ggggattagt gacagcgata aacgaaccca taaaaacaat
    26701 cagttgcccc cacagtgcgg caaatttggg catctgcaat ttaagaagct gcattgtcgc
    26761 cagcagtgga aagagaaaca gtaaaaattg ccacagatcg ctaatctgca ccatcaggcc
    26821 aaatttgacc acaaagctga atagcgaaag taacaccagc gtgcgcatca gcagtgacaa
    26881 agacttaaac ggtgctgcga cggcggagta gagtacgcag ctaattgctg ccagcgttaa
    26941 agcattggca ccggcatccc attgcgaagc aatactccat gcgccaatca tcatcaacgt
    27001 acaaaatgta cgcagcccgc tccacatagc ttcggcgtta tcggtatgac gagccaggcc
    27061 ggatgtccgt gggagtctgg catgatcatc tacaccgctt tgtagacgat gtaattcctg
    27121 actactttgc agataaaggc ggcaaaaata acgtagtcgc tgccagaagg cgatgtgccg
    27181 gtagtcggtg acgttggtcg ggcgtagtgg ggagataata cgtgcgacgg tgtaaacatc
    27241 tgtttgcgaa ctggcgagcg ccgtcagcag ctgttcgaga atttctcgcg tggcatctgg
    27301 cggtgagggc cagttgagca acatacgtcg caggctggag atgacactgg tcatacgtaa
    27361 ttgctggtgg agcagcgcat taaggcgtgc gttttgctgg cgaaaacgat agtggctcca
    27421 gaaagcctgg atacgcagca aattcatggt cagtatctgc ccaatcaccc cttcatgtgc
    27481 tgcacgaatg gcatcggttg tttcaggccg ccagagtaaa ctggcatgtt ccagtaatcg
    27541 ggcgtgcatg tttttcaatg cggttaaaag ggctgtagca tcggaactgc tcggcaggat
    27601 catcatcatc atgccgccgc acaaaatacc gactatcacc tcgcaaacac gcgcctgagc
    27661 gatatcccac agctggctgg cctcagtaat attaaccatc ggaaaggcga tgatggcagc
    27721 cgtgtagccc gccagttgaa atgcatacgc gacgttattc gtgaagtgcg cacaggccca
    27781 ggtacaaaag ccaagccacg ccgacatgct caatagaaaa aaccacggct cattgagcgt
    27841 atgtccggca agaagcagtg ctgcaatggc tccgagcaaa ctgccagcga tgcgtccgag
    27901 gcttttgctg atgacaccgc caacggtggg aaagctaacc accgcagccg aggtcatcgc
    27961 ccaatagggt tcatccagat tcaaataata ggcaaccgtc agcgccagac acatggcaat
    28021 ggtattgcgt aacgcataac gccattgcgc cagcgtggcc ctgaaccagg gcaaattgcg
    28081 caaggaccat gacgatgcgt tcattattga cctacggcaa tgctacaggt ggtgcccgac
    28141 accagcgtga catcgcctgg gactttatcc agcgtaaagc ggacgggaac gcgttgggcg
    28201 aggcgaaccc aggggacgtt aggtttcaca tccggaataa ggcttgagtc actttcaacg
    28261 ctctgatcat aaatcgcccg accgatactg gacacgtgac cctgtaacgt tttgttatca
    28321 ctatagaggg taatttgtgc tggtgcacct tcgcggatat ggcgcaactt ggtttcttca
    28381 aaataaccaa tgacgtaaaa cgagtggctg tcaacaaggg caaacagcgg tttcccggtg
    28441 tcagcgtagt cgccgatgcg ggtagtgaga ttagtcaccc atccactcac cggggcgcga
    28501 atttccgttt gcgccagttg ccattgtgcc tgtttcagcg tcgcctgtgc ggcatcaacg
    28561 ctggcctgca tcgctttaac attgaggtta gcggtatcga gttcttcggc agaaataaaa
    28621 ttttgtgaga gatgacgacg acgattcgct tcgttattgg cttttgcgag gtcagattgc
    28681 gctttcgcca gctgagcctg tgcgttcagc tcggcgatct gaaagggcgt tttatcgatc
    28741 gtgagcagaa gatcccccgc gttaaccagc tggttgtctt ttatattcag ctcaacaata
    28801 cgtcccgaca cctgtggggt gatagaaacc tgttctgcgc gtattttccc gtcgcgtgtc
    28861 cacggagatt gcatgtaata attccacagc caccatccgg caagaaccgc aactaccgct
    28921 acaatgattg ttgagaaata cttaattgtt ttaattgaca tagttaccac gcaatcagta
    28981 ttgccagagc aaggcaaacg caaatcgcaa acagcgataa atccattaac aagggatgcc
    29041 agatgtcacc agcgtagatc cagccacgaa gcaggcggtg tatgacaagc cagatgacga
    29101 atccaaacgc gaaggctttg aaaaacggag gaaagtagac ggacgcaccg aacaccagat
    29161 cttgtaaggg caatcctgtt gcattgagca taaacttcac aaagtagatt cctttacgac
    29221 agaatatatt gcgttagatt aataaatatt ctttaagtgc gaaaaattta cgcgcaattt
    29281 ctgaaaaata tgtctgacgg taaccaaatg cagcaataca tttgttttag caatacaatt
    29341 gctgcacact attctaaaag ccgcataata tcttagcaag ctaattataa ggagatgaaa
    29401 ttggaatcgc cactaggttc tgatctggca cggttggtgc gcatatggcg tgctctgata
    29461 gaccatcgcc tgaaaccgct ggagttaaca caaacccatt gggttacgtt acacaatatc
    29521 catcagttac ctccagacca gtcgcaaatt caactggcaa aagcgattgg catcgagcag
    29581 ccatcactgg tccgtacgct ggaccaactg gaagaaaaag ggttaatttc gcgtcaaact
    29641 tgtgccagcg atcgtcgggc taaacgtatt aaactgacgg aaaaggcaga gccactgatc
    29701 agcgaaatgg aggctgttat taacaaaacc cgcgcggaaa ttttacatgg catctccgca
    29761 gaggaactgg aacaactgat taagctcatc gcaaaacttg agcataatat cattgagtta
    29821 caggccaaag ggtgaaatga agggggcgtg tggtcagtaa cctgaccaca cgcaaactta
    29881 tttagcgcgg ggaaacggtc acctgactgc cattgctggc cagtacgaca cgttggcctg
    29941 gagagaaatg agtgttccct tgtttctgta ccaccatgat ggtgttgcca tcatctttac
    30001 gaatttccag ctcgacaccc tgcgttttgt tcattgcact ctgtacgccc tgaccggcta
    30061 caccacctgc aacggcccct gctgcagtag ccagagaacg cccggttccg ccaccaatag
    30121 tatttcccag gaaaccacca agtacagcac cgccaattgc gccgataacg ttggaatcat
    30181 caccgccctg aatctgtacc tgacgtacat taacgatggt gccatagctg acattctgta
    30241 cttgtttcgc ttcagaagcg gtataaacat cccctgacag ggtgtcgtta ttaacacaac
    30301 cgacaagaga cagacctacc attgaaacaa ccaatacgcg tttaatcatt gaaaaatctc
    30361 ctgttcacca tgaaacgcca cgcaagcatc cctcatggtc aaagtatatg gcatatgtga
    30421 atccatggtt acatgttcta ccaaatatga gcaaatcata atgaaagtag gatcaaccaa
    30481 ttataaacaa aatattcatg atgattattg atttgaagat aatgttttac taccaacggt
    30541 aaaaattggt cattccgcgt ggtattcacg aaaagtttat gtttgagtgg tgcgcaaacg
    30601 tcagattgaa aaaggatgaa ctatgaaatc gggccgcttt attggcgtta tgtcaggcac
    30661 cagccttgat ggtgttgatg ttgtgttagc gacaattgat gaacaccggg tcgcacagct
    30721 ggcaagtttg agctggccga tcccggtgtc tctgaaacag gctgtactgg atatttgcca
    30781 gggccagcag cttacacttt cgcagtttgg acagcttgat actcaactcg ggcgactttt
    30841 tgctgatgcg gtcaatgcct tgcttaagga acaaaacctg caggcgagag atatcgttgc
    30901 gatcggctgt cacggtcaaa ccgtctggca cgaaccgacg ggcgtggcac cacacacttt
    30961 acagattggc gataacaatc aaattgtggc gcgcaccgga attacggttg tcggtgattt
    31021 tcgccgtcgc gatattgccc tgggaggaca aggtgcaccg ctggtacctg cgttccatca
    31081 tgcgctgctg gctcacccaa ccgagcgacg aatggtgctc aatattggcg gcatcgccaa
    31141 tctgtcactg ctcattcctg gccagccggt tgggggctac gataccggtc ctggtaacat
    31201 gctgatggat gcctggatct ggcgtcaggc cggtaaacct tacgataaag atgccgagtg
    31261 ggcacgggcg ggtaaagtta ttctcccact gctgcaaaat atgctcagcg acccatattt
    31321 ctcgcaaccc gcaccgaaaa gcaccggacg cgaatacttt aactacggct ggctggagcg
    31381 ccatttgcgc cattttccgg gggttgatcc ccgtgatgtg caggccacag tggcagaact
    31441 caccgccgtg accatttctg aacaagtttt gttgagcggt ggctgcgaac gattgatggt
    31501 atgtggtgga ggtagtcgta atccgctact catggcgcgt ctggcggcat tactgccagg
    31561 cacagaagtc accaccaccg atgccgttgg cattagtggc gatgacatgg aagcattggc
    31621 gttcgcctgg cttgcctggc gaacgctggc gggattacca ggaaatctgc cttccgtcac
    31681 tggcgcaagc caggagacgg tactgggggc tattttcccc gctaacccgt gatatcagag
    31741 ttaactgaat ttttcttacc gcgtctgccg ttacacttgg aacgttaggg agggcgtatt
    31801 gccctccaga ccaggaaagt cttcgggata tgtctatgaa aaaactgtta ctgatctgtt
    31861 tgccggtgct tctcaccggc tgtagcgcgt tcaatcaact ggttgagcgc atgcaaaccg
    31921 atacgcttga ataccagtgt gatgaaaaac cgttgacggt caaactgaat aatccgcgcc
    31981 aggaagtcag ttttgtttac gataatcaac tactgcatct caaacagggc atttcagcgt
    32041 ctggtgcacg ttacaccgac ggaatctatg ttttctggtc gaaaggcgag gaagcgactg
    32101 tctataaacg cgaccgcatc gtcttgaata actgtcagtt acaaaatccg cagcgttgag
    32161 atttttccag gggcggcgca caatagcgtc acccactgac aatccgtaaa gaaaaccatg
    32221 tctgataacg acgaattgca gcaaatcgcg catctgcgcc gtgaatacac caaaggcggg
    32281 ttacgccgcc gcgatctacc cgccgatcca ttaacccttt ttgaacgttg gctctctcag
    32341 gcttgtgaag ccaaactggc tgaccctacc gcgatggtgg tcgctaccgt ggatgaacat
    32401 ggtcagcctt atcagcgcat cgttttactc aaacattacg acgaaaaagg catggtgttt
    32461 tacaccaacc tcggcagccg taaagcacat caaatcgaaa ataatccgcg cgttagcctg
    32521 ctgttcccgt ggcataccct tgaacgccag gtgatggtga tcggtaaagc ggaacgactt
    32581 tcgactctcg aagtgatgaa atattttcat agccgcccgc gtgatagcca gattggtgca
    32641 tgggtttcga agcagtccag tcgcatttct gcccgcggta tccttgaaag taaattcctg
    32701 gagctgaagc agaagtttca acagggcgaa gtgccattgc caagtttttg gggtggtttt
    32761 cgcgtcagcc ttgaacagat tgagttctgg cagggcggtg agcatcgcct gcatgaccgc
    32821 tttttgtacc agcgtgaaaa tgatgcgtgg aagattgatc gtcttgcacc ctgaaaagat
    32881 gcaaaaatct tgctttaatc gctggtactc ctgattctgg cactttattc tatgtctctt
    32941 tcgcatctgg cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac atggagattt
    33001 tgatggcaag cagtaacttg attaaacaat tgcaagagcg ggggctggta gcccaggtga
    33061 cggacgagga agcgttagca gagcgactgg cgcaaggccc gatcgcgctc tattgcggct
    33121 tcgatcctac cgctgacagc ttgcatttgg ggcatcttgt tccattgtta tgcctgaaac
    33181 gcttccagca ggcgggccac aagccggttg cgctggtagg cggcgcgacg ggtctgattg
    33241 gcgacccgag cttcaaagct gccgagcgta agctgaacac tgaagaaact gttcaggagt
    33301 gggtggacaa aatccgcaag caggttgccc cgttcctcga tttcgactgt ggagaaaact
    33361 ctgctatcgc ggcgaacaac tatgactggt tcggcaatat gaatgtgctg accttcctgc
    33421 gcgatattgg caaacacttc tccgttaacc agatgatcaa caaagaagcg gttaagcagc
    33481 gtctcaaccg tgaagatcag gggatttcgt tcactgagtt ttcctacaac ctgttgcagg
    33541 gttatgactt cgcctgtctg aacaaacagt acggtgtggt gctgcaaatc ggtggttctg
    33601 accagtgggg taacatcact tctggtatcg acctgacccg tcgtctgcat cagaatcagg
    33661 tgtttggctt gaccgttccg ctgatcacca aagcagacgg caccaaattt ggtaaaactg
    33721 aaggcggcgc agtctggttg gatccgaaga aaaccagccc gtacaaattc taccagttct
    33781 ggatcaacac tgcggatgcc gacgtttacc gcttcctgaa gttcttcacc ttcatgagca
    33841 ttgaagagat caacgccctg gaagaagaag ataaaaacag cggtaaagca ccgcgcgccc
    33901 agtatgtgct tgcagaacag gtgacacgtc tggtgcatgg tgaagaaggt ttgcaggcgg
    33961 caaaacgtat taccgaatgc ctgttcagcg gttccttgag tgcgctgagt gaagcggact
    34021 tcgaacagct ggcgcaggac ggcgtaccga tggttgagat ggaaaagggc gcagacctga
    34081 tgcaggcgct ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt aaaactatcg
    34141 cctccaatgc catcaccatt aacggtgaaa aacagtccga tcctgaatac ttctttaaag
    34201 aagaagatcg tctgtttggt cgttttacct tactgcgtcg cggcaaaaag aattactgtc
    34261 tgatttgctg gaaataatgc attaagtgga aagggggagt gagaaatcac tccccctggt
    34321 ttttatacag ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt tgtttatggt
    34381 catgcgggta acagtgcggc agagtttccg atgcgccgcc tgggcgcgaa cgtctggccg
    34441 ctgaacaccg ttcaattttc taatcacacc caatacggca aatggaccgg ttgcgtgatg
    34501 ccgcccagcc atttaaccga aattgtgcaa ggcattgccg ccattgataa attacacacc
    34561 tgtgatgccg tattaagtgg ctatctggga tcggcggagc agggtgaaca tatcctcggt
    34621 atcgttcgtc aggtgaaagc agcgaatcca caggcgaaat atttttgcga tccggtaatg
    34681 ggtcatccgg aaaaaggctg tatcgttgca ccgggtgtcg cagagtttca tgtgcggcac
    34741 ggtttgcctg ccagcgatat cattgcgccg aatctggttg agctggaaat actctgtgag
    34801 catccggtaa ataacgtcga agaagcggtt ctggcagcgc gcgaactcat tgcgcaaggg
    34861 ccacaaattg tgttggttaa acacctggcg cgagctggct acagccgtga ccgttttgaa
    34921 atgctgctag tcaccgccga tgaagcctgg catatcagcc gtccgctggt ggattttggt
    34981 atgcgccagc cggtaggtgt tggtgatgtg acgagcggtt tactgctggt gaaactgctt
    35041 cagggggcaa cgctgcagga ggccctggaa catgtgaccg ctgcagtata cgaaatcatg
    35101 gtgaccacaa aagcaatgca ggaatatgag ctgcaagtgg ttgctgctca ggatcgtatt
    35161 gccaatccag aacattattt cagcgcaaca aagctctgaa atatctcatt tcggcccaca
    35221 acgacctgtg ggccgatggc tattacttta agccttccgc tgacagcgcg tcttgtactt
    35281 ccggacgttc agccatacgt tgcataaatg ccgcaatgtg ctctaaccct tccagattca
    35341 gtttcaccgc gtatgcccag cgcagaaccg taaacagata ggcatcagca attgtgaatc
    35401 gttgcccgca gatccagtgc tcatccttca gggcctcgtt cacatattgc agcttcttct
    35461 ccagctgcgc gcgaactgtc ggtttgtact cttccggtgt atcagggcga aacagaggtg
    35521 tgaaaccttt atgcagctcg gtggcgatgt aattcagcca ttcgatggtt ttatagcggg
    35581 aaatactgtt taccggtgcc agcaactggc ggtcggggac gctgtcggca agatactgca
    35641 taatcgctac gccttccgtc agcaaagtac catcgtccag cagcaatgca ggtacctgtc
    35701 ccttagggtt aacggcaaag taatcgtcac cgttttcgag acgttttttc attaaatcca
    35761 cactgacgag cgtaaaatcc tttccgctct cacgcagggt gatatgggaa gcgagagagc
    35821 aggcgcccgg tttgtagaac aatttcatcg gtaactcctt ttgtctgtgg tttaaagtat
    35881 ggtagtgcgt tgctgaacaa aaaaaaagcc gctaacgaaa agttagcggc ttgtaaatga
    35941 gtttccctga attttacgct acggctgctt tcgccgcttt gtctgcagcg tcatcctgcg
    36001 tcatgcggtg cagtttcggc gcggtaaaca gcatcagtac tgcaataacg gcagtagcga
    36061 caccaatctg caagaatacg cgaccataga cttccagtga catcagcgga tcggtaacgt
    36121 tatccggcac agccatcata cctgcaacat aaccaccaat caggtttgca ccggcagtgg
    36181 tcaggaacca gctacccata atgaagccca tcagacgctg cggaacgagt tgtgcaacca
    36241 ttgccagacc caggccagag atcatcagtt ccccgatact ctgcaggcca tagctcgcga
    36301 ccagccagct tacagacacg ataccagcgt cagacgcgaa tttcgcaccc agcggcagaa
    36361 tcaggaacgc accagaacac atcaccatgc cgatagcaaa cttggttggc atcggcaggg
    36421 tatcgcccat cttgttatag atagcggcca gaatcgggct accaatgatg atccagaacg
    36481 ggttcagtgc ctgatactgc tcaggttcta cggccaggcc cagaatggtg tgttcaacgt
    36541 tacgaatcgc aaagaagttc agtgacgttg gcatctggct ataaagcacg aagaagataa
    36601 tggcttcgag catcaggatg aaggcaacga tcattttacg acgcgcagca cctttcatcg
    36661 cgaaggcttc tttaccgaag ataaccacaa taccgaaggc aacaacgccc agcgccatac
    36721 gcgcaacttc ctgattgtgc agcagccagg tggcgatagc gatcagtgcc acaacaccaa
    36781 taatggtcag cagcaggtta cggtagttga taggctcgaa gtctggtttt gaaccgtact
    36841 gtttaaccca acgttggcag aaggcgaagt taacgatagt gatcagcagg cctacaacgc
    36901 tcaacgcaaa cgcaacactc cagccgtatt tcgcggccag ccacggcgta gcaatcatag
    36961 agaagaaaga gccgatgttg acggacatgt agtacatggt gaatgcaccg tccagacgcg
    37021 ggtcgttttt ctcatagcat gtagaaagca gagaagacgg gttagcttta aacaggccgt
    37081 taccgaccgc aatagccgcc atacccatat aaacgatacc ggcgtcgtga ccagaccagg
    37141 caaccagagc ataaccaatc gccagcacaa tagcgccgag cataattacg cgtttagtac
    37201 ccagtacctt gtcacctaac cagccgccga tagcgaccag accataaacc agggcactaa
    37261 aggaagagaa aagggtgatt gaatccgctt cagacatacc cagttgttta accaggtaaa
    37321 cagccataat tccttgtagg ccgtaataac caaaacgttc ccataactca atcgagaaga
    37381 tgagatagaa cgccttcggt tgtttaaaag cgttcaaact gacgctttca gttggttttt
    37441 ggtttgcagt ggacactttt acctcttttt ttacatccca tattaacggg ggtgctttct
    37501 cgcgttatgt ctaaaaaaca tacgcgcaat tgttatagtg ggaggggaaa cggcaggtaa
    37561 tgttcactat cttgcacttt cagacaagtc ctttgtaata atctgttaca tataactgag
    37621 gcggggaatt caaccattcc gggaaatgtt atccagcgtt aaatttttca aaaaacgtaa
    37681 cggcagattt tttcactacc ctgagagggt gatgatggct taacggcgat atgt
//
LOCUS       DACADF010000025        37574 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.025, whole genome shotgun sequence.
ACCESSION   DACADF010000025 DACADF010000000
VERSION     DACADF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.025"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            122..850
                     /gene="mpaA"
                     /locus_tag="ISB30_001271"
     CDS             122..850
                     /gene="mpaA"
                     /locus_tag="ISB30_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAP1988924.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(825..1790)
                     /gene="ycjG"
                     /locus_tag="ISB30_001272"
     CDS             complement(825..1790)
                     /gene="ycjG"
                     /locus_tag="ISB30_001272"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAP1988925.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1909..2415
                     /gene="tpx"
                     /locus_tag="ISB30_001273"
     CDS             1909..2415
                     /gene="tpx"
                     /locus_tag="ISB30_001273"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAP1988926.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2459..4000)
                     /gene="tyrR"
                     /locus_tag="ISB30_001274"
     CDS             complement(2459..4000)
                     /gene="tyrR"
                     /locus_tag="ISB30_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAP1988927.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4148..5209)
                     /locus_tag="ISB30_001275"
     CDS             complement(4148..5209)
                     /locus_tag="ISB30_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAP1988928.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5206..6603)
                     /locus_tag="ISB30_001276"
     CDS             complement(5206..6603)
                     /locus_tag="ISB30_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAP1988929.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTEYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGMIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            6759..7757
                     /locus_tag="ISB30_001277"
     CDS             6759..7757
                     /locus_tag="ISB30_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP1988930.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTNALIESGHKNIACLHAPLDVQVSVDRVNGYKQSLATHNIAVRD
                     EWIVDGGYTHETALKAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            complement(7808..8713)
                     /gene="ompG"
                     /locus_tag="ISB30_001278"
     CDS             complement(7808..8713)
                     /gene="ompG"
                     /locus_tag="ISB30_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAP1988931.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNNWHFNIGAMYEIENVEGYV
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSLGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(8758..9840)
                     /locus_tag="ISB30_001279"
     CDS             complement(8758..9840)
                     /locus_tag="ISB30_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1988932.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFSLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGELLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     IQKIAKEMIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            complement(9854..10513)
                     /gene="pgmB"
                     /locus_tag="ISB30_001280"
     CDS             complement(9854..10513)
                     /gene="pgmB"
                     /locus_tag="ISB30_001280"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP1988933.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(10510..12777)
                     /gene="ycjT"
                     /locus_tag="ISB30_001281"
     CDS             complement(10510..12777)
                     /gene="ycjT"
                     /locus_tag="ISB30_001281"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAP1988934.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWRTSNGTGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHIDEIQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKLVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGRDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWRTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNERLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMYLKELRLPEIQPDGVLPQDDSFMAKPVINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLIYVAEESVFCLGDF
                     ILPFNGTATTHQEGE"
     gene            complement(12774..13829)
                     /locus_tag="ISB30_001282"
     CDS             complement(12774..13829)
                     /locus_tag="ISB30_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP1988935.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASLWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFAFDTQQLREQVTNGVLGEIYVTTARA
                     LRRSGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(13839..14627)
                     /locus_tag="ISB30_001283"
     CDS             complement(13839..14627)
                     /locus_tag="ISB30_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP1988936.1"
                     /translation="MKIGTQNQAFFPGNILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDHRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEAPAQAYRDSLAWLRTC"
     gene            complement(14645..15697)
                     /locus_tag="ISB30_001284"
     CDS             complement(14645..15697)
                     /locus_tag="ISB30_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP1988937.1"
                     /translation="MKKLVATAPRVAALVEYEERAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFSPRPANAPRGIEFGKFQLGNMVVGDIIECGSDVNDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGADVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(15728..16570)
                     /locus_tag="ISB30_001285"
     CDS             complement(15728..16570)
                     /locus_tag="ISB30_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP1988938.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(16557..17438)
                     /locus_tag="ISB30_001286"
     CDS             complement(16557..17438)
                     /locus_tag="ISB30_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP1988939.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(17459..18751)
                     /locus_tag="ISB30_001287"
     CDS             complement(17459..18751)
                     /locus_tag="ISB30_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1988940.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELVEQR"
     gene            complement(18765..20444)
                     /locus_tag="ISB30_001288"
     CDS             complement(18765..20444)
                     /locus_tag="ISB30_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP1988941.1"
                     /translation="MQQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQRIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFITVDP
                     KTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTNCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELDNKATLRYSVYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            complement(20655..20969)
                     /gene="pspE"
                     /locus_tag="ISB30_001289"
     CDS             complement(20655..20969)
                     /gene="pspE"
                     /locus_tag="ISB30_001289"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAP1988942.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(21044..21265)
                     /gene="pspD"
                     /locus_tag="ISB30_001290"
     CDS             complement(21044..21265)
                     /gene="pspD"
                     /locus_tag="ISB30_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAP1988943.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(21274..21633)
                     /gene="pspC"
                     /locus_tag="ISB30_001291"
     CDS             complement(21274..21633)
                     /gene="pspC"
                     /locus_tag="ISB30_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAP1988944.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(21633..21857)
                     /gene="pspB"
                     /locus_tag="ISB30_001292"
     CDS             complement(21633..21857)
                     /gene="pspB"
                     /locus_tag="ISB30_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAP1988945.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(21911..22579)
                     /gene="pspA"
                     /locus_tag="ISB30_001293"
     CDS             complement(21911..22579)
                     /gene="pspA"
                     /locus_tag="ISB30_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAP1988946.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREIEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            22746..23723
                     /gene="pspF"
                     /locus_tag="ISB30_001294"
     CDS             22746..23723
                     /gene="pspF"
                     /locus_tag="ISB30_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAP1988947.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQVSLEQGKYNQKRAAELLGLTYHQFRALLKKHQL"
     gene            23877..24014
                     /locus_tag="ISB30_001295"
                     /pseudo
     CDS             23877..24014
                     /locus_tag="ISB30_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Putrescine importer PuuP"
     gene            24148..24393
                     /locus_tag="ISB30_001296"
     CDS             24148..24393
                     /locus_tag="ISB30_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAP1988948.1"
                     /translation="MNHDIPLKYYDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24706..26349
                     /gene="sapA"
                     /locus_tag="ISB30_001297"
     CDS             24706..26349
                     /gene="sapA"
                     /locus_tag="ISB30_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAP1988949.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            26346..27311
                     /gene="sapB"
                     /locus_tag="ISB30_001298"
     CDS             26346..27311
                     /gene="sapB"
                     /locus_tag="ISB30_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAP1988950.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIISAIRHMVLPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            27298..28188
                     /gene="sapC"
                     /locus_tag="ISB30_001299"
     CDS             27298..28188
                     /gene="sapC"
                     /locus_tag="ISB30_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAP1988951.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            28188..29180
                     /gene="sapD"
                     /locus_tag="ISB30_001300"
     CDS             28188..29180
                     /gene="sapD"
                     /locus_tag="ISB30_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAP1988952.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            29182..29988
                     /gene="sapF"
                     /locus_tag="ISB30_001301"
     CDS             29182..29988
                     /gene="sapF"
                     /locus_tag="ISB30_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAP1988953.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(30036..31199)
                     /locus_tag="ISB30_001302"
     CDS             complement(30036..31199)
                     /locus_tag="ISB30_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HAP1988954.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFIHETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGTGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWIQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(31208..32581)
                     /locus_tag="ISB30_001303"
     CDS             complement(31208..32581)
                     /locus_tag="ISB30_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAP1988955.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(32604..35692)
                     /locus_tag="ISB30_001304"
                     /pseudo
     CDS             complement(32604..35692)
                     /locus_tag="ISB30_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(35692..36813)
                     /locus_tag="ISB30_001305"
     CDS             complement(35692..36813)
                     /locus_tag="ISB30_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAP1988956.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMENAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(36962..37528)
                     /locus_tag="ISB30_001306"
     CDS             complement(36962..37528)
                     /locus_tag="ISB30_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1988957.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
BASE COUNT         9063 a        10183 c         9191 g         9137 t
ORIGIN      
        1 gcaaacaact tccggcaaaa aagtgataca agatcgatga catagcgttg aagaaaagga
       61 tttgataatc cgcaaaattt gcacaacact gcctgtaata cttcctgaac cagagacgat
      121 catgaccgta actcgcccac gcgccgaacg cggcgcattt ccgcccggaa cagaacatta
      181 cggacgttca ttattgggcg cgccgttaat ctggtttccg gcccctgcag ccagtcacga
      241 aagtggtttg attctggctg gcactcacgg tgatgaaaac tcttcgatcg tcacgctctc
      301 ctgtgctctg cggactttga caccttcttt gcgccgccat catgtggtgc tgtgtgtgaa
      361 tcctgacggc tgccagttag ggttacgggc caatgctaat ggtgtggatt taaaccgaaa
      421 ctttccggca gcgaactgga aggaaggtga aacagtttat cgctggaaca gcgccgctga
      481 agaacgtgat gttgtattac tgacaggtga caaacctggc tccgaacctg aaacccaggc
      541 gctgtgccag cttatacacc gcattcaacc tgcctgggtg gtctcattcc acgatccgct
      601 ggcctgtatt gaagatccca gacacagcga attaggtgaa tggctggccc aggcgtttga
      661 attgcctctg gtaaccagcg tcggttatga aacgcctggc tctttcggca gttggtgtgc
      721 agatctcaac ttgcattgca tcaccgcaga gtttccaccc atctcctccg acgaagccag
      781 cgaaaaatat ctctttgcga tggcaaactt gctgcgctgg catcctaaag atgcaattcg
      841 cccgtcgtga actgaagcgc cggttccaca tctaccgcca gccaggtcgg tccgtcaaga
      901 tcggcgaaac tgacctgcgg caccagcggt aaagcggcgc taatggcacg agaggtacac
      961 aacatgcagc ccagcatcag acgaaaacct tgtgcacgcg cttcagtcgc cagcgccagc
     1021 gcttccgtca gaccaccggt tttatcaagc ttaatgttaa ccatctcata gcgccctttc
     1081 agcgccttca gattgctacg agtatgacaa ctttcatcag cacaaatcgg taacggatga
     1141 ataaaattct ccagcgccgc atcgtcctgc gcaggaagcg gttgttcaag catcgcaacg
     1201 cctaaatccg ccaatagctg gcaacgcgcc gccaaccctt ctgcacgcca ggattcattt
     1261 gcatcaacga tcagcgtcgc atcgggcaca gctgtgcgaa ttgccaccat ccgctcactg
     1321 ataagatggt tatccagctt cactttcagt aatttcgcgc ctgcctgcca gagtgttgat
     1381 gcactattgg ccatctgatc aggcgtaccg atgactatcg tctgtgccgt ggtgactgtc
     1441 tcgggaagcg ttatgccgat caaatcagcc agcgattgct gctgttttcg cgccgccaga
     1501 tcccacaatg cacaatccag cgcattacgt gctgcgccgg cagggaggat tttttgcaac
     1561 tcctcccgtg tcagcccttt ctctagttgc ggcacgacgc tcataatttg cgccattacc
     1621 gaggcatcgc tttccccata acgcggatac ggcgtgcatt caccggtgcc tttgataccc
     1681 tcttcttcca gttcaaccac caccacgcgc gcttcactgc gacttccccg agcaatcaca
     1741 aacggggtat gtaagggcca ggcctcctca aataccttaa cggttctcat ctttactcct
     1801 tgttgtcgcg gtggtctgcg gattaagcgc aaatagcgtt tgctgtgtta ttgacagtta
     1861 gcataaacta ggtgtgacgt taactatatg taaacaggaa agataatcat gtcacaaacc
     1921 gttcatttcc agggcaaccc ggttacagtc gccaattcca tcccgcaggc gggtagcaaa
     1981 gcgcagactt ttactctcgt ggcaaaagat ctgtctgacg tcaccctcgg tcagtttgcg
     2041 ggtaaacgca aagtgctgaa cattttcccg agtattgata ccggtgtttg cgccgcatca
     2101 gtacgtaagt ttaaccaact ggcaaccgag atcgacaaca ccgttgtgct gtgtatctct
     2161 gccgatctgc cgttcgccca gtctcgtttc tgcggcgcag aaggtctgaa caacgttatc
     2221 accctctcca ctttccgtaa cgctgaattc ctgcaagcct acggtgtggc aattgctgat
     2281 ggcccactga aaggtctggc agcgcgtgcc gttgtggtta ttgatgaaaa tgacaatgtg
     2341 attttcagcc agctggtgga tgaaatcacc accgagccgg attacgaagc agctctggct
     2401 gtactgaaag cataagttaa tatgcctgat ggtattgcac catcaggcat attcacgctt
     2461 actcttcgtt cttcttctga ctcagaccat attcccgcaa cttattggca atcgcggtat
     2521 gtgaaacgcc gagacgtttt gccagtttgc gcgtgctggg ataattgcga taaagctggg
     2581 ttaataccga gcgttcaaaa cggctggtga tttcgtccag cgaaccttcc atcgcatctt
     2641 cgcccacggc taccgttgcg gcgtcataat ccggcaacaa aatatcctgt ggacgcagct
     2701 cataaccgtc cagttgtgtc agtgcacgat agatagcgtt ctttaactgc cgcacatttc
     2761 ccggccacgc ataacgcgta agtacagtat tcagatcagc ggccagtttc ggacgcggca
     2821 cgccctgctc gtcggcaaag cgggcgacga acagctcggt taacggcatg atgtcctgcg
     2881 gacagtcacg tagcggcggc agattgagcg tcaacacgtt cagacgataa tagagatctt
     2941 cacggaacac gcctttttgc accagttcga ccagattctt ctgcgtagcg caaatcaccc
     3001 gcacatcgac atgcacctca tggtcttcgc caacccgacg gaaggtgcca tcattaagga
     3061 aacgcagtaa tttcgcctgc atccgtggtg acatctcacc tatttcatcc aacagcaccg
     3121 aaccaccgtt cgcctgctca aagaaccctt tcttcccttc cggagcatga ccaaacagtt
     3181 cactctcgac cgcatcttcc ggtatagacg cacagttcag cgccaggtaa ggtttgctcg
     3241 ctctggggct tgcctgatga caggcgtagg caaagagatc ttttccggtg ccggtatcac
     3301 ccgtaatcag cagtggcgcg cttagcatcg ccagtttctg cgcctgttcg acaacatgct
     3361 tcattttcgg gctgacagcg acaatttgac tgaaggcgct gacatcctgg gcggcgacat
     3421 tttgcaactg acggcccata cgaatcgttg atcgcagcat caccaccgca ccggtcagga
     3481 cgtgttgatc attttcatcc tgaagataaa caggcgtaat ctccatcagg aaattctgcc
     3541 cattaataac gacatgctcg ttatgcgaat cttgcggttc gctttccagc cagcgtaaaa
     3601 aattaaagcc gttaatcaat tgcgcggcgg tatggttgcg caggcgatcc aatttttgcc
     3661 caaaaagctg acagctcgcc gggttcgcca tatccacttt gcttttcata tcgacagaga
     3721 gcacaggttc aggcaacgcc tccagtaacg cgctcaacgc cagatgctca cgttcggaag
     3781 gcatccacgg gacagtacgc acatcggtaa cacctgcaat acggcgtatt tcggccatca
     3841 gactgctgaa actctcaaac tccagttcag caaaattgag gtagattcgc ccaatggggt
     3901 caatctcaat accgcgtaaa tcaatgcctc ttagcacgag tagatcgagt aattcgcggg
     3961 tcagaccgag tcggtcttca caaaagactt ccagacgcat gggaaccttc acctgaaaaa
     4021 agaacaatta atatgatgat atgacactat ttggatagca ggaaggtttc tgtcaacaat
     4081 cattgacaca aagacggaaa aacgggcact tcggcgtaaa gatatccggt cagcacccga
     4141 tattgcatta tttttcgctg ggcgttttgc ctttttgcag agtttctttc acctgaccga
     4201 taagctgacg acggaaatcc ccgaggcgtg gcttgtcatc gtcaatccac ggcagcgggc
     4261 ggcaaagctc catagcttta atcccgagtc gtgccgtcag aagtccagca ccaatcccct
     4321 gagctgcccg ggtagacaaa cgcgcagcga gatcttgcga catccagtcc atccccactt
     4381 cgcgcaccag ttcgctggct ccggcgaaag cgatattcag caataccagc ttaaacaggc
     4441 gcaaacggct gtaatatccc agttcaatgc catacagcgt ggcgatgcga ttaatcaaac
     4501 gcagattgcg ccaggcgata aacgccatat ccaccagcgc cagtgggcta acggcaatca
     4561 ttaacgttga ttccgccgct gagcggctga tttcgcgccg cgcctgggca tctaaaactg
     4621 gctggaccag gtgagcatac aaactgacca cttcacgatc gttctgcgtt tcatggattg
     4681 aggcatacca gcgctgcagc gccggatgtg actgatcgat gcccgcctgc tgcgccagtt
     4741 tttcgcagaa tgcacggcct ttacccgtgc cgtgactgtg caacaaatcg cgcgcttcat
     4801 cgcgttcatg ggcgcgctgt cgcaagcgcc ataagcgccg ccactctgtt accacagaac
     4861 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgcc acccagtcct
     4921 gggtttgcca ggcgttcatt gtccactgca cgccctgccc gacaacgctt gcgccaaaca
     4981 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcgt
     5041 ccatcaccgc ttcaacttgc ccctcttctt cctgtgcttc gtcgagcgtg gccggggcaa
     5101 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgatcga
     5161 cctccagtgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     5221 tcctattaaa aattccagcg cagcatcaag acgaatatgc ggtagcggtt tgtcgacatc
     5281 catcacctgc ggacgaaacg cctcaaactg gaagccttgc ttatcccaga acgcctgacc
     5341 tggcaaacgt gcgggaactt cgccaggata aacagtgagc ggtgcgccat cgctgagtcg
     5401 attaccgcgc agcgccggga ttttctcacc gttaacatca atcattccgc tggtggtcgc
     5461 ctgaacagac gccagcccca ggcagtccat actgatccct tcgaacgccg cattttgcca
     5521 tgcatcctga atcagttgtt gcagcaatga aaccatattg gcgtgctgat cgatggtcac
     5581 atggtccgct ttagtggcag caaacaatag cttatcgata accggcgaaa acaaacgccg
     5641 gaacagggta cgctgcccgt agtggaaact ttgcatcagc tgcgtcagcg ccagacgcat
     5701 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg
     5761 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accactttct cgcagtaata
     5821 attaaaccgc tcgcgcaaca ttccggcatt agtgtgctta tcggcctgcg ccagtttgga
     5881 ctcgccccag gcatcgacat ccggccacgg gaagaattgc agcgcgggtg ctcctgccat
     5941 atctcccggc aagacaaagc gcccaggctg aataaagtgc aacccctgct gtttgcagtg
     6001 atggagatac tcggtccagg cggcggcaat gtctgccagc cggttttcgt cggcaggcgc
     6061 tagcgggtcc agcccttcgc acatcattcg ccatttcgcc gaccattcgc cgcgctgacc
     6121 attgagtaag cccgtcatct ggcgcgacca gcttaaatag tcctgcgcca gcatcggcag
     6181 gtcgagcaac cattcgccgg ggtaatccac aatctccaga tacagcgtgg aggtatcctt
     6241 aaagtggcgc agcagcgaat cgttcgattt aaaacgtaac gccaggcgaa tttcactgac
     6301 accgcgcgta ggcgtcggcc aggcgggagg atcgccatat aactgcgcca acccttcatc
     6361 ataggtgaag cgcggaatgc caaagtcacg ctggggaatg cgttttacgc ccagcaggcg
     6421 ctcttcacgc accgcactta aaagcggcaa acgtgctccg gcatgaatat tgagcaactg
     6481 attaaccatc gcggtgataa acgctgtttt gccgctgcgg ctaagtccgg ttacagcgag
     6541 gcgcagatgt ctgtcgacac cccgattcac cagcgcattg agttcatttt taagtcgctt
     6601 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaaat cgttcgtaag gatatgaatg
     6661 tgggggccag gccattgatt atcaactacc cgtgttgtca tcgacgggcg aactccataa
     6721 gatgtgcagc attcacgttc ggacggagtt cctgacctat gtcccctact atttatgata
     6781 ttgccagggt tgcaggcgta tcaaaatcca ccgtatcacg cgtgctgaat aagcaaacca
     6841 atatctcccc ggaagcgcgc gaaaaagtgt tacgggccat tgaagaatta cagtatcaac
     6901 caaacaagct ggcccgcgcg ctgacctctt cgggttttga tgccattatg gtgatttcta
     6961 ctcgttcgac caaaactacg gcgggtaatc catttttctc tgaagttttg catgccatca
     7021 ccgccaaagc agaagaagaa ggtttcgacg tgatattaca aacgtcgcac aatccggcag
     7081 aagatctgca aaaatgcgaa agcaaaatta agcagaaaat gattaaaggc attattatgc
     7141 taagttcgcc tgccgatgaa tccttctttg cacaactcga caaatacgag atcccggtgg
     7201 tggtgatcgg taaagttgaa ggtcaataca gccatgttta ttccgtcgat accgataatt
     7261 atggcgacag tattgcgctg accaatgcgt taattgaaag cgggcataag aatatagcct
     7321 gcctgcatgc gccgcttgat gtacaagttt cagtggatcg ggtaaatggt tataagcaaa
     7381 gtctggctac gcataatatt gcagtgcgtg atgaatggat tgttgacggc ggttataccc
     7441 atgagacagc attaaaagca gcacgggaat tattaagcca gtcaccgttg cctgaggcag
     7501 tgtttgctac tgacagcctg aaattaatga gtatttatcg tgcggcggca gagaaaaaca
     7561 ttgctattcc gcagcagtta gcggtggtgg gttatagtaa tgaaacgctg tcgtttatat
     7621 taacgcctgc accaggcggc atcgatgttc cgacgcagga gttaggtcaa caaagctgcg
     7681 agttattatt ccagttaatt gccggaaaac cgtcaccaca aaatattacc gttgctacgc
     7741 atatgtcgtt gaaataaatg tatgccggat ttggcgctcg ccccgcatcc ggcaatttag
     7801 cacattatca gaacgagtaa ttcaccccaa cacccgcata atggaattta tcgctgtcgc
     7861 cttcgtcgtg atcctgccac tcaaacgcgt attccagcga aacggaaaga ccattctgga
     7921 aatcataacc gtaaaataaa cccacacggt taaaatcatg gccttcacgt tcaatatcat
     7981 cctgccagtc ccagttactc cagcgatcca gcccaatgcg cgtatacggc gtcaccgagt
     8041 ggttgccgag cgtcagcggc aaataggcgc gaatttcttg cgtggaaaac tcaccgttat
     8101 tgcggctatc atccatattg aaaccacgct cgagataata gttcactcgc aaggcaaccg
     8161 tttcgttgaa ggtatattgc agacccgttt ccgtttcgac acgggtatca gcataaccgg
     8221 tcgtattcag atcgttggca aatttataca tcgacagcca accgttgaaa cgtaaatcgt
     8281 cagtcagttt cacatcccag tctggcgcga ttttccagcg ctgcatattt gccgtgtctt
     8341 tacccggttc atcaacgtag tgataaccat agttacggaa accaccagtc agaccgagac
     8401 tgaaatcatc gctttcgagg aactgataat gtacctccag ctccgggcga tcaaaccacg
     8461 ttccacgttt acccgcgcta taatctaccg gcccttcctg ataataggcc agagaaattc
     8521 tccacggccc gttggcggca ttaaaataga cagaaggctc tgccagccca tccatatctt
     8581 cgacataacc ctcgacgttt tctatttcgt acatcgcgcc gatattaaag tgccagttgt
     8641 tcttttcctc ggcctgtgcg caggccattc ccgcacacat caccagtgcg gtacagggta
     8701 ataacttttt cattattgtt ccttgtatta tcctgggaga atgccttccc cctgtattta
     8761 gcaaattgct atttccgttt ctgcatcaaa gaaatgacat ttagtcatat caaaatgaat
     8821 agtgatattt tctcctgcat gataatcatt taacgcacca gcacggacca ctaactcgtg
     8881 ccccccaacc gtggtgtaga gcataaattc cgcaccggtt aattccgcca cgctaatttt
     8941 ggcggaaatg ttattttctt cttgcgcatc cggatgaata tcctccgggc gtattcccat
     9001 cacaatgggt ttatgtaaac tttcctgcgt tttcagaacc gctaatttct cttcgggaat
     9061 ggttaattta agtgtttccg taacgaattt atcgccatcg atcgtgccgc gaataaaatt
     9121 cattgctggt gacccaataa atccggcaac aaacatattc gccggttggt tatagaccgt
     9181 tttcggcgca ccgacctgct gaacaatccc atccttcata atgacaatcc gcgtcgccat
     9241 ggtcatcgct tcggtctgat cgtgggtcac gtagatcatg gtggtgttca gtttctgatg
     9301 cagcttgctg atctctgcgc gcatttgcac acgcagcttg gcatccaggt tagagagcgg
     9361 ctcatccatt aaaaacacgc ccgcttcgcg cacaatcgcc cgcccaagcg ccactcgctg
     9421 acgttgcccg ccggaaagcg cgcccggctt gcgtttcagg tactcacgca ggccgaggat
     9481 ttgcgccgcc cagttaaccc gctcatcaat catctctttg gcgatttttt gtatcttcag
     9541 gccaaacgcc atgttgtcgt agaccgtcat atgcggatac agcgcgtagt tctggaacac
     9601 catcgcgata ttgcgtgctt tggctggaac gtcattcatt cgtttgccgt cgatcaacag
     9661 ctcaccgccg ctgatctcct caagcccggc aatcatacgc agggtggtcg atttaccgca
     9721 gcccgacgga ccgacaaaca cgatgaactc tttatcggca atctccaggc tgaagtcctt
     9781 caccacatgc acctggttat cgtagatctt ttgaatatgt tgtaacgaaa gctgtgccat
     9841 gttgattcct ttgctatacg ttttgccaga aggccgataa ccgcggccag gtcagggagt
     9901 ccgttgaggg caacaataat tgcgccccgg ttaagcccgc gccgatcccc accgagcgca
     9961 taccgctggc gttaatggcg tcaatgcccg cctgcgcatc ttcaatgccg atacatgcct
    10021 gtggcggcac gcccagccct gcacaggcgg cgagaaagat ttccgggtcc ggtttcgaat
    10081 ttttgagttg ggaagcgtcc gcgcagaagg tgaaaaactc gcgcagctcc agcgctgcta
    10141 aaatcgtcgg cgcattcagg gagacagaag ccagcccaac tggaatctgc tgtgcccgga
    10201 gatctaccag caaattgtga atgccaggca gcacagcatt aaccgtcagt tcgcgcagtg
    10261 agtggacgta aagcagattt ttgcgatacg ccagttgcgc ccgttcctgt gggttaaaat
    10321 cgccctcttt acccccgtgc tgcagaattc gccgcaggga ctcatcgcgg ctgatccctt
    10381 ttaggaattc gttaaactgc gcatcaatgc tgatgccaat ttcagcggca atctgctgcc
    10441 acgcctggaa atgcagatgc gcggtatcgg tgattactcc atccagatcg aaaattaccc
    10501 cttgcagttt cattcaccct cctgatgcgt ggtagcggtc ccattgaagg gcaaaataaa
    10561 atcccccaaa cagaaaacag attcttcagc gacgtaaata agctgaccgt tcaaacgcag
    10621 tgaaacaggc gcagaagttc gaatcgcaat acgctgcgcg tcgagagtga cctgtaattc
    10681 gcagccctgc cagaacagag ggaaagacaa ctgttgccac tgctcaggta acgccggatt
    10741 gagatgtaat tcaccgtcac gcacgctcac cccggcaaaa ccctgaatcg cccccagcca
    10801 gatagcgcca gttgcggcag cgtggatacc gtcatcacag ctatgcggat cagcaccaag
    10861 atcgatttca gtcccctcgc gccagaactg ataactttgg gtcagcaggc cacagcgtgc
    10921 ggcaacaatg ccgtggattg ctttacttaa tgacgagtcg tgaatagtgc gcggttcata
    10981 aaattgcaga ttggcaagac acgatgccgc tgagaactgc tccggcagca tgtaattgag
    11041 catcaccaca tcagcttgtt tgaggatctg catctcgttc acttctgcgc gtgaataatc
    11101 cagcagaatg gtttgcttcc ccgccgccgc tttgtatttc gccagattaa tcaccggctt
    11161 agccataaac gaatcatcct gcggcaaaac gccgtcgggc tgaatttctg gcagccgcag
    11221 ttctttgagg tacatttctg cacgatgaat aaacgcgtcg tcgctacagc caaactgacg
    11281 agcgatattc agcgcctgct ggacgttgta atacgccata tagctggtga aggcgttatt
    11341 attgacatgt tcggtatatt cgtctggccc aataacatca tgaatttcca gacgctcgtt
    11401 aaccctcacc gcgcggctaa tccagaactt tgccgtctcc agaagtagcg ccatgccttc
    11461 atgagcaatg aaactttcat cccccgtggt ccgccagtat tgaatcaccg cccaggcgat
    11521 atcggcgacc agatggtgtt ccgcctgcgc agaggccact ttttgccgca gcccggtacg
    11581 aatgttaatg gcggcaaatt ccggcgtctc ttcttcgccg ctacgcgcgc tttcccacgg
    11641 aaacagagcg ccctgccagc cgttgcgtcg cgctttctcc tgcgcgccag gcaagttgtg
    11701 ccagcgataa cgcagtaaac ttcgggcaac cgtcggatcg ctaaacagat ggaacggtag
    11761 caaaaatact tctgtatccc agaaaacgtg gcctttgtag ccttcgccgg ttaaaccttt
    11821 cgccgcaatg ctgctgcgct catcgtgggc aggcgtcatg atgcgcaggt gataaagcgc
    11881 ataatctaat gcttgctgat cgtgcgcatc gcggccgttt accgtgatac gacgtttctg
    11941 ccaccattga cgccagtttt ctgttgatgc tgcaagaagt tggtcgtaac tctgctgcgc
    12001 gcacatttca agctgacgaa gcgacgcact gccccattcg tctaaagcgg cttgtctgtc
    12061 gtcccgccag tcgatccaca ccagtttttg cagcgtcagc gtctcacctg cgtgaagctg
    12121 cgcgcaggta tgttgcaata aacggcgctc tttggcggta tagcattgct gcacatcacc
    12181 gctcacctta cagcaacagc tgatggccac atcactacgc ccatcctggg tggtgtagat
    12241 cccctgcatc agatgctgac caaacacccg cacctggatt tcgtcaatat gctggcgacc
    12301 gtggttggtt tgcgtggcgt caatgcctgt tgaaatcagc actgacgcgt cggcgtccag
    12361 tggagtaata gtgatttcca gcgcaatgag cggcagttgg tctgccgaaa caaagcgacg
    12421 gctggcgatg gtgtaaccag tgccgttgct ggtacgccag acaacgttgc gacgtaattc
    12481 gccactggca aagttaagct cacgctgcca tgcttcgcgg gataacgaga aaatctcacc
    12541 atttatggca atctccatcc ccacgacatc aggcaggttc accagttcgt tgatttctcc
    12601 tttacccgct cgatgataca gccccgccag atacatccca cgggtctggc gcgtgtaatc
    12661 ttcttcatgg ctggcacgaa gcccaagata gccgttcccc tgcgccatca gcgaagcata
    12721 cttgttcagg gtatgctggc tgaaatgggg ttctgataac gttactggcc tggtcataat
    12781 tccacacgcg tccctgtttc cgcggattga tacaacgccg ccaccagttg ctggatgatg
    12841 tacccctgct cggcgtcggc tatcatcacg ggcttgccct gtacgtgatt gataaaggct
    12901 tccatgctgc gcaaatggcg gttgtcgtct gccatttccc gttgcatcag cgtcattaat
    12961 tcaccgttgt tatcggtgta gatatgtgcc ggaaacagcg tcgcaccggc tttatcacca
    13021 caaaagctga cattcataat cgactgttcg cggatgttga gtgcaaatga cgtttccagc
    13081 cacagaatgc cgccgttatg aaattcaatg gtgccaaaca gcgaatcttc aacgctgtaa
    13141 gttgccgggt cccactcgcc aaattgacca cagctctttt gcgtgccgat cttttgaaag
    13201 ctatgcgcat tcacgctttt caccgccgga aagcccagca catacatcgc agcatccagc
    13261 atatgaatgc cgatgtcgat cagcgggcca ccaccctgca gttctttatt ggtaaagaca
    13321 ccccagccgg gaacgccgct gcgacgcagg gcgcgggcgg tggtaacgta aatctctccc
    13381 aaaacgccgt tggtcacctg ttcacgcagc tgttgcgtat cgaacgcaaa acgatggtga
    13441 aagtcgtagg ccagcacctt gccctgtttg cgcgcggtat cgcacatttc ccgcgcctgt
    13501 tctgccgtca tggcgggcgg tttttcgcac atcacatggc agcccgcttc aagcgccatc
    13561 agggtatgtt cgtaatgaaa acggttcggt gagcagacgc taaccacatc aggtttcacc
    13621 gccagcagca tggcctgcgg atcgtcccac aaggatgcat taccgtattt ttctgccagc
    13681 gcctgcgcct gggaaaggcg gctgtcacag acggccacca gttccagatc gttgcgggtg
    13741 cagtagtacg aagcatgaac cttatccgcc acctggcctg cgccgattat cgcgacacgc
    13801 agcggagagc ttgtcattgc acttttcaca aagacctctt agcaggtgcg caaccaggcc
    13861 aacgaatcac ggtacgcctg ggcaggagct tctgcccgga tacgcccttc atacaccaca
    13921 taaccctgat agttatcggc gcgcagctgt tcaaacagcg cgtggaaatc gagggtgccg
    13981 ctgcccggct ggtaacgatg gttatcggca atatgcacat gaccgagcaa gtcgcggtgg
    14041 tcatgcagcg cctgcgccag gttatcttct tcgatgttca tgtgatagaa atcgccgata
    14101 atctgcacat gtttaagatc gttttcgacg atgtaacggc gggcatcggc gagagtattg
    14161 atcatatgat cctgataacg gtttaacggt tcgagataca ccacggttcc ggtacgcgcg
    14221 gcgacctgtt ccagtacgcg cagggaatca ctcaccattt tgcggtcgcc gtccaggctg
    14281 cgcggcgagg tcatcggcgg taaacggaag gtaaacatgc cccatgcagc aggaacgacg
    14341 atacctttac cgcccacttc tgccagcgct tcgagaatgc gttcgatctg ctttaagcca
    14401 ttaagacgac gctcttcgat aaaatcacca atccagccgt catagccgcc gcaggcagtg
    14461 gtcaccggta aaccggtttc tttgatcgcc gctttgactt cttcgatgtt gttgaccagc
    14521 agtttgccgt caatctcaaa accatcgaag cccatctctt tgatgtaacg aaatttctcc
    14581 agaatatttc ccggaaagaa tgcctgattc tgtgtgccta ttttcatttt ttcattcctt
    14641 tgaattaaaa cgtaacgccc attttgatgc tctgttccgg atgctgatcg acatactgca
    14701 tgtagctttc cgggctgttg gcaaaggtca ctaccgggtc gattaaatct tcgcaattga
    14761 gataaccgtt catcagcagt tcccagcagg tttcttcaat acgcttacgg ctccagcgcg
    14821 gataatccgg gttcggttcg ctgcacgcgc gggagaaaac aattttggcg ttattgaaat
    14881 gcgcttcgcg tccgaggtta aaaccttcag caaatggttt ggcaaacgcg acataggaga
    14941 tggtgccgcc gtaggccaga ccgcggagcg ccgactgcag cgcgtcggcg tagccgctgg
    15001 tttcgataat cacatcggca ccctgcttgc cggtcagcgt tttgatctct ttacctacgt
    15061 cagcgccaat ggggttaaga cagaaatccg caccgtggcg acgggcaata tcacagcgat
    15121 gagcgatagg atcgacacca atcaccaccg acgcgccagc gcgttttgcc agttgaatgg
    15181 caatttgacc aatcgcgcca agccctacca ccaccacaaa atcgcccacg cgcacgttgg
    15241 catcgcgcac gccgctcatg gcaaactgcg ccgggtcgta acagacggcg tttttccagg
    15301 agctgccttg cggcattttg cgcagcttgt agttattcac tgcgttaatg atgaccgtct
    15361 cggagagcgg gccgtagccg catacgctgt cgcccaccgc gtagtcgtta acgtcgctgc
    15421 cgcactcgat aatgtcgcca accaccatgt tgccaagctg gaacttgcca aactcaatgc
    15481 cgcgcggcgc atttgccgga cgcgggctga acatctgcca ttcaccgtta aagtcttcat
    15541 cgataaacgg actggcagcg cggaaatcga ccacttccgt cccgtgcttc ggagcgccga
    15601 aacgcacgcg gatcttcact tcattggcta aaatcgcccg ctcttcatac tcaaccagcg
    15661 cagctacacg cggtgctgtg gctactaact ttttcatgta aaactccttg taaagaaatt
    15721 ggtcgcttta gcccttcacg ccaccagcgg tcaaaccact cttaataaaa cgttccgaaa
    15781 gcgcatacat aatcactacc ggtagtgcgg tcaccagcga ggccgccatc atccgtcccc
    15841 agatgtaatc aggcgtactg aacagcgcgt tcaggcccac aggtaaggtg aaattgctgg
    15901 cgctggaaag gaaaatcgac gcaaacagat agtcgttcca cgccaccata aagcagtaga
    15961 cgaatacgga tatcagcccg gacattgcca ggggcacggt gatacggaag ataatttgca
    16021 gacgattgag gccatccatc attgccgctt cttcgatttc atcggggatg gtgtcgaagt
    16081 agcttttcag cataaacacg gcggtaggca gagtttgcgt caccatggtg ataatcagcg
    16141 ccatctcggt gtcgtaaatg ccaagtgcgg tgatgatttt gaacagcggc accaccagca
    16201 aaatgccgga gaacatgtac accgtgtaaa agctggcgtt gatggtcatt cgccctttaa
    16261 agcgcaggcg ggaaagcgcg taagccccaa gaatgccgag aaataccgcc accacggatg
    16321 aaaccaccga caccaccatg ctgttacgga agtagtcgac aaacgggaaa atcaccggat
    16381 taaaaatgtc gacgtaatgc tccagcgtcc actgctgtgg cagcaacgta ggatgcagcg
    16441 agatcgcctc ttttgcgctc ttgaacgagg tcatcagcat cacaaaaaat ggaaacagcg
    16501 tgatgatgag aaacagcgcc agcccgcagt aaaaaccgat tcgactgagt gtgcgtttat
    16561 ttgttgccat tgaggttcac ccgtttcctg gtcagcagaa tgacagcgaa aataatgatg
    16621 aaaagcacca ccgagatcgc cgccgctttc cctaagtcat taaaggcaaa ggcggttttg
    16681 tagagataga caccgagaat atcgaccttg gtcgtcagca gataaacatc cgcgaacatg
    16741 tagaacatcc agatagtgcg cagggtcacc accgtcgcca gaaccggcat aatcgcgggc
    16801 agcgtgacga tgcgaaaccg ttgccaggca ttcgcgccat ccatctctgc cgcttcgtac
    16861 agcgatttgt cgatggtctg caaaatcgcc agaaacgaga taaaggcata cgggaagtag
    16921 cgccagatgg cgaacagcac caccagcaca aaactactgc ccgggttatc gaaccacagc
    16981 ggcgcctgct catagagatg cagaagatcg acgccgaggt agttcacaat gccgtagccg
    17041 ttgttgaaca tgtatttcca ggcgaacacc agcgaaatag acggcgttac gtaggaaagg
    17101 atcaccagcg aacgcgcggt tttgcgcagg cggaactcgc ggttgaaaaa catcgccacg
    17161 gccagcccga gcacggtgct gcccgccacc acaagcgcgg tataccagac cgtcatccac
    17221 agcgagtgcc agaaaccagg atcggagaga atgcgcacat agttactcac cccaacaaac
    17281 gttgactcga tgttgggatt aagcggcaga cgtaaaaaac tgatttcgat attcgacacc
    17341 atcggccacg ccaccagacc gcccagcagt aataagctgg gcgcgagaag caacagcgca
    17401 aagggcatat cggaacgacc tgaaaaaagc ctgttcattg cggattcctg tcctgggttt
    17461 agcgctgttc gaccaactca tccagctttt tctggctcgc ttgcagcgtg ccagggaggt
    17521 cggctttacc cacggtgacg ttatgcacca ttgaactcac cacaccagac cccgtcacat
    17581 cacccatgcg ggtaaagttt ttatccccta ctgcgccaaa gacctgaata tttggcaatt
    17641 cgctgattag ctgattcggc aattctccca gcgccttaat aacgtcgttg tctttccagg
    17701 tggcggtagt gacaaccgct ttgttcaccg gcagcgccgc gcctggtgac atcatcaccc
    17761 agtcggcaat gttgtccgcc tgctccataa aggtgacaaa tttttcagcc gcttcggtct
    17821 cttcagtctt ttgcccggcg gtaatggtca gcgaggtcaa catgccgtag accgcagaat
    17881 ttttctcggt tggcaccacg aaaccaacgt ttttcggatc gccttcttta atcacagccg
    17941 gaaggatata ggtggagtaa atcgccatcg gcgcggtgcc gttcataaaa gcgtctttca
    18001 cttccatgat gtcgttagaa cctggcatgg tgttggcagc aaggttgcgg taataggtca
    18061 gcgcctgcat catctctggt gtatcaaggg tgattttgcc ttcggcgtta aagacgttag
    18121 cctgattgga taacgcaaac tgggagaaag actgttccgt caacacgctt tctgctgtag
    18181 gcagcgcaat accgtatttt ttattcgccg ggtcattcag tttctgtgca acgtccagca
    18241 attgctgcca gttttttggc tcctccagcc cagcttttgc cagcacatct ttgcgatacc
    18301 agatcccgcc aatccatgcg ctgacaggaa caccggtcca ggcgctgcca tcttcggtgc
    18361 gcacaatccg cagtacgcca tcgtagaacg cgccttcacc gacgttgctg atgaccgtgg
    18421 caaccgcttt acgatcgata agctgctctt tgtccatcac tttggcgtag tcatggctgg
    18481 tttcgatcac ttccggcagc gaaccgctac gtgaaagggt aatgactttg gtgttatagg
    18541 cgtcttcttc cacgggcact tgttttacgg tgatacctgg gttttctttt tcaaaacggg
    18601 caatcaattt gctgatgacg gcctggcgct cctgctccac cgaagaatgc ataaattcga
    18661 tggatacatt atttttattt tcttctttac agcctgaaat cagggcgcag gaaaccagtg
    18721 ctgataacag cacgattttt gatttaatca tgaaatgttc ctttttattt aatccacata
    18781 acctgccagg gatgcagtgt tatttcatta cccaatatat caacttcact aatcagatcc
    18841 cgaccgtgca ggtcagtaat atttatatgc tgaatatttt cgctgacatt aaacaatccc
    18901 gtcaggcaat cgccatccgc attgcttctt tgaattcgca ttacaaatga attaattgca
    18961 tcgatggtga aattattatc cggatgaaac tctttatgac tgcgacgaag ggtaattaaa
    19021 tgcgacaact tataatatac cgaatacctt aatgtcgctt tattgtccag ttctgtcgtt
    19081 atctctttac tagagtattt tttacggtta atcgcgcgat tatatccaag tttttcgaca
    19141 ccggcgtaat cattacgcga ccccagaatg ctttgaatat atatcgctgg cacaccgggg
    19201 aaacttaaca aaatcgcatg ggcgagaata aacctggcac accgttcttc gtcgctacac
    19261 tcacggcggc tcaacgcatc catataggtg acattcattt cataggggct acgcgtaccg
    19321 tcgggattat ttttccagtt tactaatgcc ccttcctgct gtaacacctc gaccagcgct
    19381 aatatttcgt tttcgggtaa caacccccgc agtgggttaa gcccgatgcc atcgtgagag
    19441 gcaaggaagt taaaccaggt ggtgttgctg gaaggtagtg tcaggttttg cgcccacgca
    19501 caaagtgcct caacgttctg tttttgcacc gcatgcagca ccagcggcgg cagcgagaac
    19561 tggtacacca tatgcgcttc gtcatcgcct tcgccaaagt aagcaatgtt gtctttatgc
    19621 ggaacattgg tctcggtaat gatcactgtg cctggcgcga cgttatcaat aatcgaacgt
    19681 aacagtttga taatcagatg tgttttttcc agatggatgc agtttgttcc cggctctttc
    19741 cacataaagc caacggcatc cagacggaca tactctgcgc ccttttccag gtagcacagc
    19801 aatacatcca ccatcgccag caacacttca gggctacggt aattgaggtc aatttgatcg
    19861 tcgctaaagg tggtccataa atgacgcgtt gaatgatcgc gcatctggaa tggcgttaat
    19921 aacggtaacg cacgcgggcg agtgacggcg ctgagatcgg ttttcggatc aacggtaata
    19981 aaaaaatctt caaaacctgg ctgctgttgt aaatagtttt taaaccattc actttttgcc
    20041 gacatatggt tgcagacaaa atcaaacatt aaatggctgc attcaccgag ttgctgaata
    20101 tcctgccact cccccgcttc actggcgacc tgatgataat caattaccga aaatccatca
    20161 tcagatgacc atggataaaa aggtaataaa tgaacatgtg aaaaaattct ttgcagccat
    20221 tgagggtaaa actgattaaa tgtgggtagt ggctttaaat cattactgtg aaattgatcg
    20281 gcataggtaa ttaacacgac atcactttca tcccagtgtt ttttacgtcg ctgtgtaatt
    20341 aatcgtttcg cactctcaag acgcgttaca agtttctgta aatgagtcgc ggtaaatgtt
    20401 ccaccgtaga tttcgtccag gtaatccgta attttctgtt gcatgttgcc tttccgtggt
    20461 agcggtccca tgaagcgaga gactactccg cataaaataa actgtcaatt gagctcaagg
    20521 ggtttagtca cgcaggaaat ctttttttac ctgggaaaaa gcgtatttgt gagctgacgc
    20581 aaataatcta tggggatgta aataaggtgt gacagaaaaa aacggcgcat aagcgccgct
    20641 catggtgaat tcttttaacc tttgaccttc ggcattgcga tgtctttcag gccaccggcg
    20701 ttctcaacgt gggtatatcc catctcgcta aggatctctt ttgcctgccc tgactggcgt
    20761 ccggcattgc aatacacttt cacggtgtcg tttttatccg gaacggcggt agcaatgcgc
    20821 tctttcactt ccttcagggg aatattgatg gccccctgaa cgtgctcttg ctgatactgc
    20881 tctggaacac gaacatcgat ccagtgttca gcggcgaaaa cgggcagtga aaacaccagt
    20941 gtcagagcaa gtaagccttt tttaaacatg taagcgtcct tctatggatg aaaattgcta
    21001 tccattatac gcctgagatt taaccatttt tgcgaaactc ccctcacctt ttataacgct
    21061 gcgccagttt attggcagcc cgacttaaca gcggttccag cgccacagcc agcaacattt
    21121 tcagcggtcg gcgagcaact gattttatcg cccagcccgc cacacccgcc gggccatagc
    21181 gcagtgcggt aagaagtacc agcttgcctg ctaatttgaa cccaggcttc accttttgcc
    21241 cggcctgttg ccagcgagta ttcataactt tcctcacagt tgacggaaac ggctacgcag
    21301 cgtgaaagtg tcggaagtga cataacgttc catctcgcgt aaacgcgttt cacttgccgc
    21361 cagttcacgg tcgacttcat ccagcaattc gctgctggaa ggtagctgct caccaaaggc
    21421 catgttgtcc ggcattggat caagcgcaaa tgacaaaatg atgtaagcaa ccagggtaaa
    21481 cagcgccaga ccgaagaaaa tcgacagcac caccaggata cgcaccagtt ttactggtac
    21541 atcaaaatag ttggcaatcc cggcgcagac gccacggacc atgccctgct gcggaatacg
    21601 ccataatttt ttattgagat taatgcccgc cattagcgat ccctccagtt cggatgttct
    21661 gcatcaagaa tggattccag cgcctgaata cgctcgcgca tccgttttgc ttcatcagcc
    21721 agttgcgcta atcgctgctg ctcactttgt gacaattcac tgcgaccgga acgattgctg
    21781 taatgcagcc ataaccagat cggtaaaaca aacagcacaa aaatggttaa cggaatagcc
    21841 agaaatagcg cgctcataag tactccttag acgatgagcg gcggcgcgtt cggacgccgc
    21901 ctggatgtta ttattgattg tcttgcttca ttttggcttt taattgtgcc agttgctcgc
    21961 tgattgcatc atcggctttc agttcggcaa actgatcgtc cagcgatttt tgtttaccga
    22021 agctgtggct ttctgcttcc gcttccatct ggtcgatacg gcgttcgaaa gattcgaaac
    22081 gagccattgc ttcatccagt ttgccactgt ccagctgacg acgcacatcg cgcgacgagt
    22141 ttgccgcttg atggcgtaac atcaatgcct gctggcgagc gcgtgtttcg ctcaatttgt
    22201 tttccagctc gccgatctct ttcttcatac gcactagcgt atcgtccacc agcgtcactt
    22261 catgttccag gctcttaatc agatcggtca gtttctgttt ttcaattaac gccgcacgcg
    22321 ccagatcctc tctctctttc agcagtgcca gttccgcttt ttcctgccat tcaatttcac
    22381 gagccgacgc ttgttcaata cggcgagtca gctgtttctt ttctgccagc gcacgcgccg
    22441 aagtagaacg tacttcaacc aatgtatctt ccatctcctg gatcatcaga cgcaccagtt
    22501 tctgtggatc ttccgctttc tctaacagag cgttgatgtt ggcattcacg atgtcggcaa
    22561 agcgagaaaa aatacccata atgttgtcct cttgatttct gcgttcagga ttgtcctgct
    22621 gaactgttaa tacaatttgc gtgccaattt tttatctttt tgatttataa agatctgatt
    22681 gaagaatcaa cagcaacatg ccaggatgaa ttagcgaatt acactaacaa gtggcgaatt
    22741 tcatcatggc agaatacaaa gataatttac tcggtgaggc gaacagcttt ctcgaagtgc
    22801 tggaacaggt ttcgcatctc gcaccgctgg acaaaccggt gctcatcatc ggcgagcgcg
    22861 gcaccggtaa agagctgatt gccagccgtc tgcattatct ctcctcccgt tggcaagggc
    22921 cgtttatttc ccttaactgc gcggcgttaa atgaaaattt gctggattcc gaactgtttg
    22981 gtcacgaagc gggggcgttt accggtgcgc aaaaacgtca tccagggaga tttgaacgtg
    23041 ccgacggcgg tacgctattt cttgatgaac tcgctacggc accgatgatg gtgcaggaaa
    23101 aattattgcg tgtgattgag tacggtgaac tggagcgcgt cggcggcagc cagccattgc
    23161 aggtgaatgt gcggttggta tgcgcgacga atgccgatct cccggcaatg gtcaatgaag
    23221 ggacttttcg cgctgacctg ctcgaccgac tagcgttcga tgttgtgcaa ctgccgccac
    23281 tacgcgaacg tgaaagcgac attatgttga tggcagaaca ctttgccatc cagatgtgtc
    23341 gggaaatcaa gctgcctctg ttcccgggtt ttaccgagca cgccagagaa acattgctga
    23401 attatcgctg gccgggaaat atacgtgaat tgaaaaacgt ggtggaacgt tcagtgtatc
    23461 gccacggcac cagcgattat ccgcttgatg acatcattat tgatcccttt aaacgccgtc
    23521 catctgaaga agccatcgcc gtttcagaaa acacctcgct tccgacgctg ccgctggatt
    23581 tacgcgagtt tcagatgcag caggaaaaag aactgcttca ggtcagcttg gaacaaggga
    23641 agtataacca gaagcgcgcc gctgaattgc tggggttaac ctatcatcag ttccgcgcgt
    23701 tgctgaaaaa gcaccagctt tagcatttcg acaatgcaac ttgccgggtg tgccgtgaac
    23761 gccttatcag gcctacaaaa tcaatacgtc gtttgcaggc ctgataagcg ctgcgcatca
    23821 ggcattattg cgttaaccct gcttctgcgt attaatctct cagcgcatcc tgcaaaattt
    23881 tcattgccgc atcgaattgc gcaaccggga tggtatgggc ttcgttaggc ggcgcatatt
    23941 tgtggtattt gatccgccgc tatcgcaaag tgccattgta tgatggcgac agaacgccgg
    24001 tgagcgaaac gtaatagtcg aatgcgcggc gcattatcct gcctgagggt aatgcaccgc
    24061 gccattaact ataaaaatat gtatgacccg gatcacgagt gctatcatcc gcccttatgc
    24121 ttagcgcatt aaccccaggg gcaaataatg aaccacgata tcccgctaaa atattatgat
    24181 attgccgatg agtacgcgac cgaatgcgca gagcctgtcg cagacgctga acgcacaccg
    24241 ctggcccact acttccagtt actgctcacc cgtttgatga acaatgaaga gatcagtgaa
    24301 gaagcccaac atgaaatggc tgctgaagcg gggattaatc ccgtacgcat tgatgagatc
    24361 gcagagtttt tgaatcaatg gggtaatgag taattattta attgcagact atcggtcagt
    24421 atcgttgccc ggataaagcg ttcgcgccgc atccggctaa aaatgacacg cactgcagcg
    24481 tgctttaatg ggtttctcac gatcgtaccg ctctgctcct cgctaagttg ttctgtgagt
    24541 tatagttata aagaaatcac gggtacgaat gttgaactga tggcgacgcc acctgttcaa
    24601 tgaccaacca cacggccctc attattacct ctgaacacaa acggtaatgg caaatgtcat
    24661 taaatattag taagatagcc tctttacata aaatccctta atattatgcg ccaggtatta
    24721 tcgtctcttt tggtgattgc tggacttgtg agtggtcagg caatcgccgc gcctgaatcc
    24781 cccccgcatg ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg gcaagtcaac
    24841 acctttaacc catccaaagc gagcagtggg ttaattgtcg atacccttgc cgcccagttt
    24901 tatgatcgac tgctggatgt cgatccctat acctatcgtc tgatgccgga acttgccgaa
    24961 agctgggaag tactcgacaa cggcgcaacc tatcgcttcc acctgcgtcg cgatgttccg
    25021 tttcaaaaaa ccgactggtt tactcccact cgtaaaatga atgccgacga tgtggttttt
    25081 accttccagc gaatttttga ccgcaacaac ccgtggcata acgttaatgg cagcaacttc
    25141 ccctacttcg acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa actggataat
    25201 cataccgttg agttccgtct ggctcagccg gatgcttctt ttttgtggca cctcgcgacc
    25261 cattatgctt cggtcatgtc ggcagaatat gcagggaagt tagagaaaga agatcgccag
    25321 gagcaactcg accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga ataccgcgcc
    25381 gggcaatata ttcgcttaca acgtcatgat gatttctggc gtggtaagcc gttgatgcca
    25441 caagtggtgg tggatttagg ctctggcggt acaggacgat tgtcgaaact cctgaccggg
    25501 gagtgcgacg ttctggcctg gcctgctgcc agtcagctat ccattttgcg tgacgaccca
    25561 cgcttgcgtt taacgctgcg tcctgggatg aacgtcgcct atctggcatt caacaccgcc
    25621 aaaccgccgc taaataatcc cgctgtccgc catgcgctgg cactggcaat taataaccag
    25681 cgcctgatgc aatccatcta ttatggtacg gctgaaacag cggcctctat tttaccgcgc
    25741 gcctcgtggg cctatgacaa cgaggctaaa attactgaat acaatccggc aaaatcgcgc
    25801 gaacagttga aggcgttggg gctggaaaac ttaacgctga aactgtgggt gcccacacgt
    25861 tcgcaggcgt ggaaccccag tccactgaaa accgccgaac tgattcaggc ggatatggca
    25921 caggttggcg taaaagtggt gattgtgccg gtagaaggtc gttttcagga ggcgcggttg
    25981 atggatatga gccatgatct gacgttatcc ggttgggcga cggacagtaa cgacccggac
    26041 agtttcttcc gtccgttact gagctgcgcg gcaattcatt cgcagaccaa cctcgcccac
    26101 tggtgcgatc cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca gcagctggcg
    26161 gcgcgtattg aagcctatga cgaagcgcaa agtattctgg cgcaggaatt acctattctg
    26221 ccgctggcgt cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg tctggtactt
    26281 agcccgtttg gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga tgaggtgaaa
    26341 aagccatgat tatcttcacc ttacgccgta ttttgttatt gattgtgacc ttgttcctgc
    26401 tgaccttcgt tggcttcagc ttgagctatt tcaccccaca tgcgccgtta caaggcgcgt
    26461 cactgtggaa tgcctgggtg ttctggttta atggcctgat ccactgggat tttggtgttt
    26521 ccagcattaa cggtcaaccg attgccgaac agttaaaaga ggtcttcccg gcaacgatgg
    26581 aactgtgcat cctcgccttc ggttttgcgc tgattgttgg catccccgtg gggatgattg
    26641 ccgggattac gcgccataag tggcaggata acttgatcaa cgccatcgcc ttactgggct
    26701 tttcaatccc ggtgttctgg ctggcgcttc tgttgacgct gttttgttca ctcacgctag
    26761 gttggttgcc cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg attaccggtt
    26821 ttgcgttgat tgatgcctgg ctttcggact caccgtggcg ggatgaaatg atcattagcg
    26881 caatccgcca tatggtattg cccgtgatca ccctgtcggt tgcgccaaca actgaagtga
    26941 tccgactgat gcgtatcagc actatcgaag tgtatgacca gaactatgtt aaagcagcgg
    27001 cgacccgcgg tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat aacgcgttac
    27061 ctccggttat tcctcgcctg ggcttacagt tttccaccat gctgacgctg gcgatgatca
    27121 ccgaaatggt ttttagctgg ccgggtttag ggcgctggtt aattaacgcc atccgccagc
    27181 aggattacgc agccatttcc gccggagtga tggtgtgtgg ctcactggtc attattgtta
    27241 acgtgatttc tgatattttg ggtgccatgg ctaaccctct gaaacataag gaatggtatg
    27301 ccttacgata gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac cgcctggcgc
    27361 aaattttata gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg gctggctgta
    27421 ctgtgtattt ttggcggctg gtttgcgcct tacggcatcg accagcaatt tctcggttat
    27481 caattattgc cgccgtcatg gtcgcgctat ggcgaagttt ctttcttcct ggggactgac
    27541 gacctcggac gcgatgtttt aagccgttta ctgagcggag ctgcgccaac cgtaggtggc
    27601 gcatttgtgg tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac cttcgccggg
    27661 gcgacccacg gcctacgctc agcggtgctt aaccatattc tggatacctt actggcgatc
    27721 ccttcgctgc tgctggcaat tatcgttgta gcgtttgccg gaccgagttt gtctcacgcc
    27781 atgtttgctg tctggctggc gctgctgcca cgtatggtac gttcgattta cagcatggtg
    27841 catgacgaac tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc atcaacgctg
    27901 aatattctct ggtttgctgt gatgcccaac atcaccgccg ggctggtcac cgagatcacc
    27961 cgcgcactgt cgatggcaat tctcgatatc gccgcgctgg gctttctcga tctcggcgca
    28021 caactgccct cgcctgaatg gggagcgatg ctcggtgatg cgctggaact gatttatgtc
    28081 gccccgtgga ctgtcatgct gcctggtgcg gcgattatga ttagcgtgct gttggttaac
    28141 ctccttggcg acggtgtccg tcgtgcaatt attgcggggg tggaataatg ccattactcg
    28201 atattcgtaa cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa gccgtcgacc
    28261 gcgtaagtat gaccttaacc gaaggtgaaa tccgcggtct tgttggcgaa tccggttccg
    28321 gtaaaagttt gattgcgaaa gcgatttgtg gggtgaataa agataactgg cgtgttactg
    28381 ctgaccgtat gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc gaacggcgca
    28441 agctggttgg tcacaacgtg tcgatgattt tccaggaacc gcagtcgtgt cttgaccctt
    28501 cagaacgtgt gggtcgccag ttgatgcaaa acatcccagc ctggacctat aaaggccgtt
    28561 ggtggcagcg ttttggctgg cgcaaacgcc gtgccattga actgctgcac cgcgtgggga
    28621 ttaaagatca caaagatgcg atgcgcagtt ttccctatga gttgaccgaa ggcgaatgtc
    28681 agaaagtgat gatagccatt gcactggcga atcaaccgcg tctgctgatt gctgacgaac
    28741 cgacaaactc aatggagcca acaacccagg cgcaaatctt tcgcctcctg acgcgtctca
    28801 accaaaacag caataccact attttgctta tcagccatga cttacaaatg cttagccaat
    28861 gggcggataa aattaacgtg ctttactgcg gtcaaacggt ggaaaccgcg ccgagtaagg
    28921 agttggtgac gatgccacac catccttata cccaggcgct gatccgcgcg ataccagact
    28981 tcggcagcgc gatgccgcat aaaagtcgcc tcaatacgct gcccggcgct atcccgctac
    29041 tggaacagtt accgattggt tgtcgtctgg ggccacgttg cccgtatgca caacgagaat
    29101 gcattgtgac gccacgtttg acgggggcga aaaatcatct ctatgcctgt catttcccac
    29161 tgaacatgga gaaagagtga gatgatcgaa acgctacttg aagtgcgtaa tttaagtaaa
    29221 acgttccgct accggaccgg ctggtttcgt cgtcagaccg tagaagcggt aaaacccttg
    29281 agctttacgc tacgtgaagg ccagacactg gcgattatcg gcgagaatgg ttcgggtaaa
    29341 tccacgctgg caaaaatgct ggcgggaatg atagaaccta ccagcggcga gttattgatt
    29401 gacgatcatc cgctgcattt tggcgattat tccttccgca gtcagcgtat tcgcatgatt
    29461 tttcaggatc cttccacctc gttgaatccc cgtcaacgta tctcgcaaat tctggatttt
    29521 ccactgcgcc tgaacaccga cctggaaccg gaacagcgtc gcaaacagat tattgaaacg
    29581 atgcgtatgg ttgggctgct accggatcac gtcagttact atccgcatat gttggcaccc
    29641 gggcaaaaac agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa agtcattatt
    29701 gccgatgaag ccctcgcctc actggatatg tcgatgcgtt cgcagttgat taatctgatg
    29761 ctggagttac aggaaaaaca gggcatttcg tatatttatg ttacccagca tatcggaatg
    29821 atgaagcaca ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt cgagcgaggc
    29881 agcaccgcag atgtgctggc atcgccgctg catgaactca ccaaacggct gatcgccggt
    29941 cattttggcg aggcattgac ggcggatgcg tggcgtaagg accgttagtg ttttgcacct
    30001 catcctgaca ggcccccctc aggatgaggt gttcattact tttggttgtc gattctggtg
    30061 agagcaacca gcaatgccag cgtataacac accgtaattg ccagactcat ttttaacagt
    30121 gtttctccgc caattcccga caatggcgcg gctataccgc cgaagacaaa catgctcatc
    30181 cccatcaacg cagacgccgt tccagattcc tgcgtcccta ctgcactcat agcctctgcc
    30241 ccgccaacag tgccgatgcc gctacaaaat gcgatggtga aaaataatgc caccagtgcc
    30301 ggaatgggca attgtatcca tgcgcataag accgtgagca atgcacacaa aattgccgct
    30361 atcaggccac ctcgcaacaa tgtcatcgcg ttaatccgtc gcgccaggcg cgagaagatc
    30421 catgaagcaa tgataagtcc ggtgccgtta aggccaaaca ccaggctaaa ttgcattgga
    30481 ctaaagccaa attccttctg caacacgaac gacgaagagc cgatatatgc aaacaaaccg
    30541 gccagcataa aactttgaat caggcaaaag cgcataaaac ggcggttctg caccacgctt
    30601 cgtccggtta atagcaatga tgagcccctt ttattttctg gcaaggtctc atgaataaat
    30661 aacagacagc ccagcaacag tacggtgcta atttcagcca ttacccagaa taaagtgcgc
    30721 cagtcaaaag tgctaacaat gtacccgccc agcactggcg acaacaccgg tgccaggcca
    30781 ttcaccgtca ttaacagcgc aaaaaactgg gtcaacgtta ctccctgata tttgtcacga
    30841 gcaatagaac gagagagcac cgaaccaccc gcccccgcga tcccttgaat aaaacgccag
    30901 accaccagcc agtaaatatt gttcgttgtc gcgcataaaa tggaagaaac aataaacaat
    30961 agcaacgaca agattaacgg acgtttacgc cctattttgt cgctcagagg gccaaataac
    31021 agttgtccga cgccaagacc aatcagtgat gcggtcaggg ttaattgcgt tatggtcgtg
    31081 gttgccgcca gttgctcaga catctccggc aaagccggca aataaagatc ggtacacatc
    31141 gggccgatac cggctaaaag accaagaatc aatgcccatg aaagagagac tctagccata
    31201 ttattcctta cttgagtgat gacataccgc cgcccagcga ctgccagagc gtgattcggt
    31261 ttgtaaaatc agtcagttgc agtgccagca gctcctgctg tgcgctccac aagctgcgct
    31321 gcgccgtcaa cacggtcagg taatcaccaa cacccgcctg atagcgacgc aagccaacat
    31381 cgaccgtttg ttgttcagct ttaacatact ggcgctgtgc atccagttgc tcttcaagcg
    31441 tggtccgccg cgccagtgcg ttagcgacat ctttaaatgc gctctgaacg gttttttcat
    31501 aggtggcaat gagtccacgc ttttgtgctt ctgcgtaacg aagctgcgcc agattacttc
    31561 cgccagtaaa cagcggtaat gtgaccgatg gcgcaaatga ccagatctgc attccgtggc
    31621 tgaacaacga cgacaacgcg tcactgccaa caccggcact ggcagtcagc gaaatcgtcg
    31681 ggaaaaagtt agcccgcgcc gcgccaatat ctgcattggc gctcttcaaa ttatgttccg
    31741 cttcctggat atccgggcga cgcagcagaa catcagatga tactcccgca ggaaccagac
    31801 ttatcatttg ttccggcagg ctttccagtg ttcccggtag cagattttcc gcaagcgttg
    31861 tacctgccag taagttcagc gcatttttat cctgcatcac ttgtgtctga tagctggcaa
    31921 cgctggcgcg cgcttgctga taaacactca tcgcctcact gacatctgtc gctgccgccg
    31981 tgccaacctg ttgctggcgc tggataattt tcaatgagtt ttctgcactg gccatcgttt
    32041 cttttgccag ggccaggttg ctgttatcag ccgccagtgt cagccaggcg gtgctgattt
    32101 cagcaatcag cgttaaacga gtgttctggg ctgtaaattc actggcaagc caggtttccc
    32161 gcgccgcccg agataaactc tgattacgac caaacagatc taacgtataa ctggacaccg
    32221 tgccgtcagc ctcggcagtt gttcccgtac cattcgccag cgaacggctg cgggtcgaac
    32281 ttaaggccgc attcaccgtc ggaaataacg acgcattagt ttgcccatag agcgcccgtg
    32341 ctgagtcaat atcagcaatc gctttttgca catcgcggtt actgttcagt gcaatggtta
    32401 ctacctgctg caatcgggga tcgtggatca cctgctgcca gtcatggctg attgccttcc
    32461 cctggccctg tgctcccggc aaggtcgccg ggattgggga ttcaggcgta ctgtagtgag
    32521 gatcgagcga gacacatccc gcactcaaca acaccagaaa aataagagaa cgacgcaaca
    32581 taacttactc ctgacggcgc ggtttaccgg caaacaaacg tttcaccagt acaaaaaaca
    32641 gaggaacgaa gaaaatggcc agtaatgtag cggtcagcgt gccgccaata atgcccgtac
    32701 caatggcgat gcggctgttc gcccctgcgc cggttgctat cgccagcggc attacccccg
    32761 caataaacgc cagcgaggtc atgatgattg ggcgtaaacg agtctgcgcc gcgcgtaatg
    32821 ccgcacgact caaggaatag ccctctgcaa ccgccgcttc agcaaattcc acaatcagaa
    32881 tggcgttttt cgacgacaag ccgatagtag ttaatagcgc tacctggaag taaacgtcgt
    32941 tgtttaaatc acgcatccag gccgccagcg ccgcgccaag aagccccaga gggatcacca
    33001 gaatgaccga gaacgggact gaccagcttt cgtacagtgc tgcaaggcag aggaaaacca
    33061 ccagaataga aactgcatac agacttaatg cctgaccgct ggccagtttc tcctgcaatg
    33121 acaaaccact ccaggcccag gttgttcccg caggaagact gtttgccagt tcctccattt
    33181 ttgtcattgc atcgccggaa ctaaagccag tcgcgttttc gccctgaatt tcataggctg
    33241 ccgagccgtt gtagcgcacc agtctttccg gtccatacag ccagcgggtg gtcgcaaaag
    33301 cagagaatgg tgtcatggcg ttatcgctgc cgcgcacaaa ccatttacct aagtctgacg
    33361 gcgcggaacg aaattcgctg tcgccctgaa tgtagacttt tttcacacga ccacggtcaa
    33421 taaagtcatt cacataagta ccgccccacg cactggacag ggtgtcggtg acatcattca
    33481 aactcaaccc taatgacacc gctttattac tatcaatatc tacctgcaat tgcggcattt
    33541 gtggcaaatc attggcgcgt acagaatgta actccggact ttgattcgct ttttctatca
    33601 actgattacg catttgcagc agtgtttcac gatcggttcc accgttagct aacaattcaa
    33661 acgtaaaacc attgctttgg cccagcccat caacggctgg cggcgtcatc gcaaataccg
    33721 tggcatcacg aattgtgccc agctctttgg ttgcccgtag ggcgatagcc tgggcggtgt
    33781 tttctgcccc tttacgttga gaccagtttt tcaacgaaac aaacgccatc ccggtgttct
    33841 gtccgctacc gctaaaactg aaaccatcaa cggtaaagat gacatcggta tttgctttct
    33901 cgttaatcag gaaccagtca acaatctggc gattcacttc tgctgtacgg gcagcggtcg
    33961 ccccggccgg cagcgtgtac tgcaccatga tttcgccctg gtcttcagtg ggtaagaaac
    34021 tgcccggcag tttccacatc atcagggcca ttccaccacc cagcacgaca taaagcccca
    34081 tcgttcgggc tgcacggcgc aggacataaa ttacgcctcg ctgatattta tcttcagtac
    34141 ggcggtaaaa gcggttaaat gcgccgaaaa agcctttttt atgtggtgga acatgctgga
    34201 ggacggaacc gcacagggcg ggagtcaagg tcaatgccac caccacggaa agcagcattg
    34261 cggagatgat ggtgatggag aactgacgat aaattacccc cgtagatccc ccaaagaagg
    34321 ccatcggcag gaatacggct gacaacacca gcgcaatggc aaccagtgcg ccagaaatct
    34381 cgcccattga tttttccgtg gcttcacgcg cgggcaaccc ttcgtcacgc ataatgcgtt
    34441 cgacgttctc taccaccaca atggcatcgt ccaccagcaa gccgatcgcc agcaccattg
    34501 caaacaacgt cagggtatta atcgaatagc caaacaacgc aagaacgccg aatgtgccca
    34561 gcaaaacgac ggggacagct aacgccggaa tcagtgtggc acgcaggttt tgcaggaata
    34621 aatacatcac gcaaaccacc aatacgatgg cttcaaacag cgtctgaatt acgtcctcta
    34681 cggagatttt aataaattca gtgctgtctt tcgggtatgc gatgtcgtat ccctgcggca
    34741 tgttccgctg gaattcggca atcttatcct tgaccagcgt cgccgtattc agcgcattcg
    34801 caccgggcga aagcattaca gcaaccccgg cagacgggtg accgttaagt ttgccgatag
    34861 cggtataatc ttcactgccc atctcaacgc gagccacatc ttttatacga acaactgcgc
    34921 cgtctgactg gcttttcacg ataatatttt tgaactgatc caccgtctgc aagcgagact
    34981 gcgcacgtac cgttgcggtc agttgctgag tatccggtga aggcaatgcc cctattttcc
    35041 cggcagtaac ctgcacgttt tgcgcttcaa tagcactttg cacgtctgaa ggcatcagcg
    35101 agtaagacgc cagtttggcc gggtcaagcc agatgcgcat agcgtattcc gcgccaaaga
    35161 cttgcagact ccccacacct tcaacacgcg ccagcgggtc ctgaacgtta ctgaccagcc
    35221 agtcggcgat atccgaactg gaggctttgt cagtggtgtc atacacggca gcaatcagca
    35281 aaaagttgct ttgtgatttc tccaccgtaa cacccgtttg ctgcacttcg ctgggtaggc
    35341 gcgactccgc ctgctgaatt ttattctgca cctgaacctg tgcagtatct ggatcggtac
    35401 cttgttcaaa ggtcacatta atactgaccg aaccatcaga gctactggtt gagctgaaat
    35461 agagtaaatt atcaagccca gtgagttgtt gctcgataac ctgagtcacg ctgttctcca
    35521 gcgtttcggc agaagcacca gtataagtgg ctgaaatttt aatggtcggc ggtgcaacgt
    35581 caggatattg cgcgacgggc aatgtgcgaa tagccagaat ccctgccaac ataatcaaaa
    35641 tggcgataac ccaggcaaag accgggcggc gaacaaaaaa gcgcgaaaac atcaggcgtt
    35701 gcctccatta gcctgaactt caacagcctt gacggtctgc cctgaagtga ctttggcaga
    35761 accttcaaca atcagtcggt cgccgttgtg caggccgttc agcaccagcc atttatcacc
    35821 atacgtttct cccgtttcga gcgttcgctg ctctacttta ttgtctttat tcaccaccag
    35881 cgcagttgca ttgcctttag catcgcgcgt aacgccctgt tgaggcgcga gaatagcgtt
    35941 ttccatcacg ccttcatcga cacgagcgcg aacaaacatt cccggtaata actgctgttg
    36001 tggattgggg aaaattgccc gtaatgtcac cgaaccggta gactcatcca ccgcgacttc
    36061 ggtgagttcc agacgccctt tttcgctgta ggttgtgcca tcttccagaa taagtgagac
    36121 gctcatggtg tcactgttgg tcgccagtga ctgtttacgc agacgtaata aatcgacact
    36181 ggagcgagtg aggtcgacat acattgtatc cagaccacga atagtcgtta acgctgtatc
    36241 ctgcgacgcg gtcaccagtg cgccaggggt taccgacgaa ataccaatac gccccgaaat
    36301 cggtgcggtt accgtggtcc agtcaagatt aatgcgcgca gtttccagtg cggctttttt
    36361 cgcctctaca ctggctttat cttgtgcaca ggtagactga gcatcatcag catcctgttg
    36421 tgaaacacca ttctctttca ccagtcgggc ataacgctgc gctttctggc aatcggcttt
    36481 taccagtgcc tgtgcttgtt gtaatgctgc ccgggcttca ttccatgcag cctgataact
    36541 ggccgcatca atctggtaga gaggctgtcc agccttgacc agatcacctt ccttaaataa
    36601 gcgtttctgg ataattcccc caacctgcgg acgtacttca gcactgagcg cagcactggt
    36661 gcgtccggtt aattcactga ctaccgaaac cggttgagat ttgagcgtaa caacgccgac
    36721 ttctgtttct gacggggatg aattgttact ttgcgtatta tcacaacccg ataagagcag
    36781 cattgccact acagatgttg ctatatactt cattattttc tccagggtga gaaataccct
    36841 cctcattgaa attaatgagg atatgtcagg gatattcagt tataacgaac ccgcatcgcg
    36901 aaatgaattt cgcaatgaaa taagggggat taatatcccc cgctattggt taattctgtt
    36961 cttatttatt tatcaacata ctaacaatat cctgatatat ttcctgcaga tgttgtggat
    37021 cgcttttttg cggtgttaaa cgacgataaa ccgtaccttc catcatggta gcaaacactt
    37081 caacgcagca acgaatgtgc tcgtcgctaa gatggggatg cattcttttc atatgtgcac
    37141 aaccgttagc gaacattctg gcgtcagctt cttcaagcat tgccataacc cgcgggttgc
    37201 gtgtggcttc tgcagccact tccagcatca gcgcgtcgtc gtcctcattt aaggatttac
    37261 gcaaggcaag gacttccggg aagtgatcgg tacgcgcgtc aatgtccatt tgggcaatac
    37321 gaaagtcgat gatacggcga accatctctt caataatggc gtctttgttg gcgaagtaac
    37381 gatatatctg acccacgcta agctgagctt cgctggcaat ttgcgacata ctggcggcat
    37441 gaaatccgca gcgacgaaaa caccgacggg cggcgttaat gatttcatcc tgacgctgtt
    37501 tgtggcgtat ctccagcttg ctggtcattt ctgtctcctg gcctatcact tggtgagaat
    37561 gatcattctc attt
//
LOCUS       DACADF010000026        37258 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.026, whole genome shotgun sequence.
ACCESSION   DACADF010000026 DACADF010000000
VERSION     DACADF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.026"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            161..1531
                     /gene="aroP"
                     /locus_tag="ISB30_001307"
     CDS             161..1531
                     /gene="aroP"
                     /locus_tag="ISB30_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAP1988958.1"
                     /translation="MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAG
                     PGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLY
                     VLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
                     IAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLEL
                     VGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFH
                     ELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVN
                     TILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGV
                     VTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKTA
                     KAVKAH"
     gene            complement(1574..2428)
                     /gene="ampE"
                     /locus_tag="ISB30_001308"
     CDS             complement(1574..2428)
                     /gene="ampE"
                     /locus_tag="ISB30_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAP1988959.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     CMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHAHATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHALDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2425..2976)
                     /gene="ampD"
                     /locus_tag="ISB30_001309"
     CDS             complement(2425..2976)
                     /gene="ampD"
                     /locus_tag="ISB30_001309"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAP1988960.1"
                     /translation="MLLEQGWLAGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3064..3957
                     /gene="nadC"
                     /locus_tag="ISB30_001310"
     CDS             3064..3957
                     /gene="nadC"
                     /locus_tag="ISB30_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAP1988961.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4161..4601
                     /gene="ppdD"
                     /locus_tag="ISB30_001311"
     CDS             4161..4601
                     /gene="ppdD"
                     /locus_tag="ISB30_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAP1988962.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4611..5996
                     /gene="gspE"
                     /locus_tag="ISB30_001312"
                     /pseudo
     CDS             4611..5996
                     /gene="gspE"
                     /locus_tag="ISB30_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            5986..7188
                     /gene="hofC"
                     /locus_tag="ISB30_001313"
     CDS             5986..7188
                     /gene="hofC"
                     /locus_tag="ISB30_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAP1988963.1"
                     /translation="MASKQLWRWRGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7223..8266)
                     /gene="guaC"
                     /locus_tag="ISB30_001314"
     CDS             complement(7223..8266)
                     /gene="guaC"
                     /locus_tag="ISB30_001314"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP1988964.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQRW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            8491..9111
                     /gene="coaE"
                     /locus_tag="ISB30_001315"
     CDS             8491..9111
                     /gene="coaE"
                     /locus_tag="ISB30_001315"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP1988965.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9111..9854
                     /gene="zapD"
                     /locus_tag="ISB30_001316"
     CDS             9111..9854
                     /gene="zapD"
                     /locus_tag="ISB30_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAP1988966.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9864..10061
                     /gene="yacG"
                     /locus_tag="ISB30_001317"
     CDS             9864..10061
                     /gene="yacG"
                     /locus_tag="ISB30_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAP1988967.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10149..10547)
                     /gene="mutT"
                     /locus_tag="ISB30_001318"
     CDS             complement(10149..10547)
                     /gene="mutT"
                     /locus_tag="ISB30_001318"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAP1988968.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFSGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRVYVE"
     gene            complement(10607..13312)
                     /gene="secA"
                     /locus_tag="ISB30_001319"
     CDS             complement(10607..13312)
                     /gene="secA"
                     /locus_tag="ISB30_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP1988969.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEIEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13374..13886)
                     /gene="secM"
                     /locus_tag="ISB30_001320"
     CDS             complement(13374..13886)
                     /gene="secM"
                     /locus_tag="ISB30_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAP1988970.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLETNTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14116..15033)
                     /gene="lpxC"
                     /locus_tag="ISB30_001321"
     CDS             complement(14116..15033)
                     /gene="lpxC"
                     /locus_tag="ISB30_001321"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAP1988971.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15134..16285)
                     /gene="ftsZ"
                     /locus_tag="ISB30_001322"
     CDS             complement(15134..16285)
                     /gene="ftsZ"
                     /locus_tag="ISB30_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP1988972.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16346..17608)
                     /gene="ftsA"
                     /locus_tag="ISB30_001323"
     CDS             complement(16346..17608)
                     /gene="ftsA"
                     /locus_tag="ISB30_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP1988973.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17605..18435)
                     /gene="ftsQ"
                     /locus_tag="ISB30_001324"
     CDS             complement(17605..18435)
                     /gene="ftsQ"
                     /locus_tag="ISB30_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAP1988974.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18437..19357)
                     /locus_tag="ISB30_001325"
     CDS             complement(18437..19357)
                     /locus_tag="ISB30_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP1988975.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQSAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19350..20825)
                     /gene="murC"
                     /locus_tag="ISB30_001326"
     CDS             complement(19350..20825)
                     /gene="murC"
                     /locus_tag="ISB30_001326"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP1988976.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20879..21946)
                     /gene="murG"
                     /locus_tag="ISB30_001327"
     CDS             complement(20879..21946)
                     /gene="murG"
                     /locus_tag="ISB30_001327"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP1988977.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEVGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQSQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21943..23187)
                     /gene="ftsW"
                     /locus_tag="ISB30_001328"
     CDS             complement(21943..23187)
                     /gene="ftsW"
                     /locus_tag="ISB30_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAP1988978.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23187..24503)
                     /gene="murD"
                     /locus_tag="ISB30_001329"
     CDS             complement(23187..24503)
                     /gene="murD"
                     /locus_tag="ISB30_001329"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP1988979.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24506..25588)
                     /gene="mraY"
                     /locus_tag="ISB30_001330"
     CDS             complement(24506..25588)
                     /gene="mraY"
                     /locus_tag="ISB30_001330"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP1988980.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25582..26940)
                     /gene="murF"
                     /locus_tag="ISB30_001331"
     CDS             complement(25582..26940)
                     /gene="murF"
                     /locus_tag="ISB30_001331"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP1988981.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLVNLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26937..28424)
                     /gene="murE"
                     /locus_tag="ISB30_001332"
     CDS             complement(26937..28424)
                     /gene="murE"
                     /locus_tag="ISB30_001332"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAP1988982.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSAHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATICFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVVVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28411..30177)
                     /locus_tag="ISB30_001333"
     CDS             complement(28411..30177)
                     /locus_tag="ISB30_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAP1988983.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30193..30558)
                     /gene="ftsL"
                     /locus_tag="ISB30_001334"
     CDS             complement(30193..30558)
                     /gene="ftsL"
                     /locus_tag="ISB30_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP1988984.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30555..31496)
                     /gene="rsmH"
                     /locus_tag="ISB30_001335"
     CDS             complement(30555..31496)
                     /gene="rsmH"
                     /locus_tag="ISB30_001335"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP1988985.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31498..31956)
                     /gene="mraZ"
                     /locus_tag="ISB30_001336"
     CDS             complement(31498..31956)
                     /gene="mraZ"
                     /locus_tag="ISB30_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP1988986.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32558..33562)
                     /gene="cra"
                     /locus_tag="ISB30_001337"
     CDS             complement(32558..33562)
                     /gene="cra"
                     /locus_tag="ISB30_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAP1988987.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33644..33730)
                     /locus_tag="ISB30_001338"
     CDS             complement(33644..33730)
                     /locus_tag="ISB30_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAP1988988.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33742..34233)
                     /gene="ilvN"
                     /locus_tag="ISB30_001339"
     CDS             complement(33742..34233)
                     /gene="ilvN"
                     /locus_tag="ISB30_001339"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAP1988989.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34236..35960)
                     /gene="ilvI"
                     /locus_tag="ISB30_001340"
     CDS             complement(34236..35960)
                     /gene="ilvI"
                     /locus_tag="ISB30_001340"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAP1988990.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEIGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGARFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(36278..37222)
                     /gene="leuO"
                     /locus_tag="ISB30_001341"
     CDS             complement(36278..37222)
                     /gene="leuO"
                     /locus_tag="ISB30_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAP1988991.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9000 a        10399 c         9069 g         8790 t
ORIGIN      
        1 gcattctcgc gcgtatacca ttatcttgtg atagttgaaa gtactatcat cataattcag
       61 ttacataaaa tgtaaagaaa ccaatacaac aaacggaatt acaaaattac acacgcattg
      121 ctgcgtagtt caaaaaaaca acacaacacg aggtttcata atggaaggtc aacagcacgg
      181 cgagcagcta aagcgcggcc ttaaaaaccg ccatattcag cttatcgcgc tgggtggcgc
      241 gatagggaca gggctattcc tgggtagcgc ctccgtaata cagtccgcag ggccagggat
      301 tatcctgggt tacgccattg ctggttttat cgcctttctg atcatgcgtc agctaggtga
      361 aatggtggtc gaagaacctg tcgcaggctc ctttagccac tttgcttata aatactgggg
      421 cagttttgcc ggtttcgcct ctggctggaa ctactgggta ctgtacgttt tagttgccat
      481 ggcagagctg actgccgtgg gtaaatacat tcagttctgg tatccggaaa tcccaacctg
      541 ggtttctgcc gccgtgttct ttgtggtgat caacgccatc aacctgacaa acgtaaaagt
      601 gtttggcgag atggagttct ggtttgccat tatcaaagtt atcgcggtgg tagcgatgat
      661 catcttcggc gcctggctgc tattcagcgg caacggcggc ccgcaggcga gcgttagcaa
      721 cctgtgggat cagggtggtt tcctgccaca cggcttcacc ggtctggtga tgatgatggc
      781 gattatcatg ttctctttcg gtggtctgga actggtgggg atcaccgcag cagaagctga
      841 taacccggag caaagtatcc cgaaagcaac taaccaggtt atctaccgca tcctgatttt
      901 ctatattggc tcgttagccg ttctgctctc actaatgccg tggacccgcg ttaccgccga
      961 taccagtccg tttgtgctga tcttccacga gttaggcgat acctttgtag cgaatgcgct
     1021 gaatatcgtg gtactgactg ctgcgctctc cgtgtacaac agctgcgtat attgcaacag
     1081 ccgtatgctg ttcggtctgg cacaacaagg gaacgcgcca aaagcgctgg cgtctgtcga
     1141 taaacgcggc gtaccggtta acactattct ggtgtctgcg ctggttacgg cattgtgcgt
     1201 actgattaac taccttgccc cagagtccgc tttcggactg ttaatggcgc tggtggtatc
     1261 tgcactggtc atcaactggg cgatgatcag tctggcgcat atgaaattcc gtcgtgccaa
     1321 acaagaacaa ggcgtggtaa ctcgcttccc tgccctgctt tatccgctgg gtaactggat
     1381 ctgcctgctg tttatggcgg cggtactggt gattatgctg atgaccccag gaatggcgat
     1441 ttcggtatac ctgatcccgg tatggctgat cgtgttaggt atcggctatc tgtttaaaga
     1501 gaaaaccgcc aaagccgtaa aagcgcatta atctctctac gccctcaccc gtgcagggtg
     1561 agggcaataa tctttacacc aacgccccgt aaatcgtcag tagcgcaatt accaccacga
     1621 ccacgaacga ggttttcttc gccattgaaa ccgcggcctt tggcgtctcc accttatcga
     1681 catgcggttc acgcgccaga gagaactgtg ccagacgcgt taatacctga tattgcgaag
     1741 tatggaaatc acccagcgaa gcaaaccagg ccggtaacgc tttctcacca tgaccgatca
     1801 aggcatatac cacacccgca agacgaaccg gcacccaatc cagtgcatga agcacggcat
     1861 caatgccgga ctgtaaacga tgatgcggcg tctggtatcg cgccagccag tattgccatg
     1921 cacgcaaaaa cgcatacccc atcagcgtaa cgggtcccca ggttcccccc acaatcagcc
     1981 agaacagcgg agcaagataa aaacgaaagt taatccacag caatgcattt tgcagctcac
     2041 gcaaatactc acgttcatcg cagcctgccg ggacgccgtg aatcatggtg agttcgccag
     2101 ccatcgtggc atgggcatgg ctatcattac gtgaagcagc tgtcagataa gcatgataat
     2161 gaagacgaac tttacctgcg ccaatacaca gcaaaccaat cagcagccac accagcaacg
     2221 tgggaacgtt gaacaataca ccctgcaatg cgcgtaacag taaaaaagtc acgcccatcg
     2281 caataatggt catgcataac gtgcgcccga gagaaaaatg tttcacccgc cgaaagaacg
     2341 cttcaagacg atgatcaagc tgccagtgct cgcccaactt aaacaggcgc tcgaaaatta
     2401 acaccagtaa ggttgtaaat agcgtcatgt tgtctccttg ctgaccagcg cacgaaaccg
     2461 tgcccaatca aatgcaggac cgggatcggt tttccgatcc ggcgcaatat cacaatggcc
     2521 cgtcatgtta ttagcgatat ccggatagcg atcaatcagt gcccgtgtaa ccgccgcaag
     2581 ctgttgatac tgcgcatcgg tatacgccag cgtatcggtg ccttccagct caatcccaat
     2641 agaaaaatca ttgcagcgtt cgcgcccctg atactgagag actcccgcat gccaggctcg
     2701 tttatcgaaa ggaacatact ggactatttc accatcacgg cgaatcaaac agtgagcgga
     2761 gacgcgcaaa tgggcgatct ccgcaaagaa aggatgcgcc tgcggatcaa tagttccagt
     2821 gaataatgcg tcgatccacg gaccgccaaa ctcgcctggc ggcaggctaa tattgtgcac
     2881 caccagcagg gtgggtgttt cgtcatccgg gcggcaatcg taatgtggtg agggaacgcg
     2941 gcgcgcgcca gccagccacc cctgttctaa caacatgcag gatctcctta tatgtggtgc
     3001 taatacccgg ttcagagtag catgtttcta ccttatgatt cgttagctat ctggagtttt
     3061 aacatgccgc ctcgccgcta taaccctgac acccgacgtg acgagctgct ggaacgcatt
     3121 aatctcgata tccccggcgc ggtggcccag gcgctgcggg aagatttagg cggaacagtc
     3181 gatgccaaca atgacattac ggcaaaactt ttaccggaaa actctcgctc tcatgccaca
     3241 gtgatcaccc gcgagaatgg cgtattttgc ggcaaacgct gggttgaaga ggtgtttatt
     3301 caactggcag gcgacgatgt caccataatc tggcatgtgg atgacggcga tgtcatcaac
     3361 gccaatcaac ccttgttcga acttgaaggc ccatctcgcg tgctgttaac gggcgaacgc
     3421 actgcgctta attttgtgca aaccctttca ggagttgcca gtaaggtacg ccactatgtc
     3481 gaattactgg aaggcaccaa cacgcagctg ttggatacgc gcaaaacctt acccggcctg
     3541 cgttcagctc tgaaatacgc ggtactttgc ggcggcggag cgaatcaccg tctggggctt
     3601 tctgatgcct tcctgatcaa agaaaaccat attattgcct ccggctcagt gcggcaggcg
     3661 gtcgaaaaag cgtcctggtt gcacccggat gcgccagtag aagtcgaagt agagaatctg
     3721 gaagaacttg atgaagccct gaaagcaggt gccgatatca tcatgctgga taacttcgaa
     3781 acagaacaga tgcgcgaagc cgtcaaacgc accaacggca aggcgctact ggaagtgtct
     3841 ggcaacgtca ctgacaaaac actgcgcgaa tttgccgaaa cgggcgtgga ctttatctcc
     3901 gttggtgcac taactaaaca cgtacaggca ctcgaccttt caatgcgttt tcgctaattg
     3961 ctaacagatt tatccgctcc atcgcgggcg gataaatctc cccttcccca actttcttcg
     4021 taacgcctcg caaatttctt gtctttcgct gcaacgaagt aaaaaacctc ggaagctgct
     4081 ttcccgtttc gttttttgcc cctcgcttgt gctgatggcg tttgccaaag tagcgccaac
     4141 caaatcaagg agcgaaacag atggacaagc aacgcggttt tacacttatt gaactgatgg
     4201 tggttattgg catcattgcc attttaagcg ccattggtat tcccgcttat caaaactacc
     4261 tgcgcaaagc cgcactcacc gacatgctac aaacctttgt gccttaccgt accgccgtag
     4321 agttgtgcgc gctggaacat ggtggattaa atacctgcga cggtggcagc aatggcattc
     4381 cttcgcctac caccacccgc tatgtttcag ccatgagtgt ggcaaagggc gtggtgtcgc
     4441 tgaccgggca agaaagtctc aatgggctaa gcgtcgtcat gacaccgggt tgggataacg
     4501 caaacggcgt caccggctgg acgcgtaact gcaatattca aagtgacagc gcattgcagc
     4561 aagcctgcga agatgtcttc cgctttgatg acgccaacta aggagcggca atgaatattc
     4621 cacagctcac tgccctgtgt ctgcgttatc agggagtctt gctggatgcc agcgaagagg
     4681 tggttcatgt tgcggtagtc gatgcacctt cgcatgagct actggacgca ttgcatttcg
     4741 ctaccaccaa acgtattgag atcacctgct ggacgcgcca acaaatggaa ggtcacgcca
     4801 gtcgcacaca acagacattg cccgtagctg ttcaggagaa gcatcagccc aaagcagagt
     4861 tgctgactcg aacgttacaa tctgcgctag aacaacgcgc gtctgatatt catatcgaac
     4921 cagcggacaa tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat cctttaccgg
     4981 acgtttcacc ggatgccgga gtcgcattaa ccgccagatt aaaagtgctg ggaaacctgg
     5041 atattgcgga acatcgcctg ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg
     5101 ccgtctcatt tcgtattgcg accttagcat gtcggggtgg tgaaaaggtg gtattaaggt
     5161 tgttacagca ggtgaaccag gcactggatg ttaacacgct tggaatgcag ccgtcacaac
     5221 tggtggactt tgctcatgcc ttgcaacaac cacagggact ggtgctggta actggcccta
     5281 caggcagcgg caaaacggtc acgctttata gtgccctgca aacgctgaat accgctgaca
     5341 ttaatatttg tagcgtcgaa gatccggttg agatccccat agccggatta aaccagacgc
     5401 aaatccattc gcgtgccgga ctcacctttc agggcgtttt gcgtgcgtta ttgcgccagg
     5461 atcctgacgt catcatgatc ggagagatcc gcgatggcga aacggcagaa attgccataa
     5521 aagccgcgca aaccggtcac ctggtgttgt ctaccctaca cactaattcc acctgcgaaa
     5581 cgctggtacg tttacagcaa atgggagtcg cccgctggat gctatcatcg gcgcttacgc
     5641 tggtaatagc ccagcgtctg gtacgcaaac tttgcccgca ttgtcgccgg cagcaagggg
     5701 agcccatcca tattccagac aatgtatggc cgtcgccgct gccccactga caggcacccg
     5761 gttgtgtaca ttgctaccac ggtttttatg gtcgcacggc cttatttgaa gttctgccca
     5821 taacaccggt catacgtcag cttatttccg ctaataccga cgttgaatcg ctggaaacgc
     5881 acgcccgaca ggcgggtatg cgaacgcttt ttgaaaacgg ctgcctggct gtggagcaag
     5941 gcttaaccac ctttgaagag ttaatccgcg tattggggat gccgcatggc gagtaagcaa
     6001 ctctggcgct ggcgtggcat aaccggcgac ggcaatgcgc aagatgggat gctatgggca
     6061 gagagccgtg ctttgctgct catggcacta cagcaacaga tggttacccc actaagcctg
     6121 aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata aaagcgcgga agtcattcat
     6181 caactggcga cgctactcaa agccgggtta acgctttctg aagggctggc tctgctggcg
     6241 gaacagcatc ccagtaagca gtggcaagcg ttgctgcaat cactggcgca cgatcttgaa
     6301 cagggcgttg ccttttccaa tgccttatta ccctggtcag aggtatttcc gccgctctat
     6361 caggcgatga tccgcacagg tgaactgacc ggtaagctgg atgaatgctg ctttgaactg
     6421 gcgcgtcagc aaaaagccca gcgtcagttg accgacaaag tgaaatcagc gttacgttat
     6481 cccattatca ttttagcgat ggcaatcatg gtggttgtgg caatgctgca ttttgttctg
     6541 ccggagtttg ccgctatcta taagaccttt aacactccgc taccggcgct aacgcagggg
     6601 atcatgacgc tggcaagttt tagtggcgaa tggggttggt tgctggtgct attcggcttt
     6661 ctgctggcga tagccaataa gctgctgatg cgccacccaa ccaggcttat cgcacggcag
     6721 aaattactgt tacgcatccc gattatgggt tcactgatgc ggggacaaaa actcacgcag
     6781 atctttacga ttctgacgct gacacaaagt gcaggcattt cttttttaca gggcgttgag
     6841 agcgtcagag aaacaatgcg ctgtccgtac tgggtgcaac ttctgacaca aatccagcac
     6901 gatatcagta acggtcaccc catctggctg gcgctaaaaa atgccggtga gtttagcccg
     6961 ctctgtttgc aattagtgag aacaggagag gcatccggct cgctggatct catgttagac
     7021 aacctcgccc atcatcatcg ggataacaca atggcgctgg cggataacct cgcagcctta
     7081 ctggaaccgg cgttgctgat cataacggga ggaattatcg gtacgctggt ggtggcaatg
     7141 tatctgccaa ttttccattt aggcgatgcg atgagtggaa tgggataacg ctggcgtgtt
     7201 gctccacgcc agcgttggta tattacaggt tgttgaagat gcggttttct tgttcctgca
     7261 cacggataaa tgtggtgcgc ttggtcagtt ctttcaggcg tgaagcccca acgtatgtac
     7321 aagctgaacg caggccgccc aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg
     7381 gcagcttaac ggttttacct tctgctgcgc gatattccgc aacaccgcca acgtgacgtt
     7441 tcatcgcaga ctctgagctc atgccgtaga acagcataaa tttctcgccg ttctcctcaa
     7501 cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg
     7561 caccgccgcc aaaggctttc gcgacatcgc ccggcgtggt gcagccaccg tcgctgacga
     7621 tcattccgcc cagaccatgc gcagcatcgg cacattcgat caccgcagaa agttgcggat
     7681 aaccgacgcc tgttttgacg cgagttgtac aaacagaacc tgggccaatg ccaactttaa
     7741 cgatatcagc accggagagg ataagctcct cacacatttc accagtcact acgttgccag
     7801 cacaaatggt tttggtcggc cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt
     7861 cggaataacc attcgccacg tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa
     7921 tctgtttagt tttttcgaaa tccgcatcag acgtgccggt agaaaccatc acatgtttca
     7981 gcacatcagc ggaagaattg ttgataaacg cttgccactc ttcgacagaa tagtgtttat
     8041 gcacagcagt caaaatatca aaagaagcca gcgcagaggc catagaaaat gtgcctacgg
     8101 tgtccatatt ggcggcgata attggcacgc cggaccagcg ctgacctgaa tgtttgaagg
     8161 tgaattgacg ttccagttca acatcggaac ggcttttaag agtggagcgt ttagggcgga
     8221 tgagaacgtc tttaaaacct aacttcagat cttcttcaat acgcatgtgc ggattcctgg
     8281 ggttaatggc tataagggta agagcgcaac tccagtgacg ttatcatacg cactaatcaa
     8341 tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca atcccataaa tttacctggt
     8401 aaatagtgag cgaacgtttc atcaatttcg ctttcgtgcg ctcattttta acgttttgat
     8461 aatcatgatg aattcccggg aatataaatt atgaggtata tagttgcctt aacgggaggc
     8521 attggcagtg gcaagagtac cgttgccaat gcgtttgctg atctcggaat taacgtcatt
     8581 gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct acatgccatt
     8641 gctgatcact ttggcgctaa catgattgct gctgatggaa cattgcagcg ccgggccttg
     8701 cgcgagcgga tcttcgccaa cccggaagag aaaaactggc ttaacgccct gctgcatccg
     8761 ctgattcagc aagagacgca acaccagatc cagcaagcta cttcccccta tgtactatgg
     8821 gttgtgccat tgctggtaga aaactcactg tataaaaaag cgaatcgagt gctggtggtg
     8881 gatgtcagcc cagaaacgca acttaagcgc accatgcagc gcgacgatgt aactcgcgag
     8941 catgtcgaac aaatccttgc tgctcaggca acgcgcgaag ctcgccttgc cgtggcagat
     9001 gacgtcattg ataataacgg cgcaccggat gctatcgcat cggatgttgc ccgcctgcac
     9061 gcacactatt tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc
     9121 aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt
     9181 tgattcagca actcaccgtt aatttaccca tcgttgacca cgctggcgcg ctgcatttct
     9241 tccgtaatgt cagcgaatta ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt
     9301 tgaaagaact tgaccggcag caacgtaaac tccagacctg gattggcgtg cccggcgtgg
     9361 accagagccg tattgaagca ttaattcagc agttaaaagc ggcggggagc gtattaattt
     9421 ccgcgccgcg tatcgggcaa tttctacgtg aagatcgttt gattgctctg gtgcgtcagc
     9481 gactgagcat cccgggcggc tgttgcagct ttgatttacc tacattgcac atttggctgc
     9541 atctacccca ggcgcagcgc gacagccagg tagaaacctg gattgccagc ctgaacccac
     9601 ttacccaggc acttaccatg gtgctggatt taattcgcca gtcggccccc ttccgtaaac
     9661 aaaccagcct gaatggtttt tatcaggata acggtggcga tgccgacttg ctgcgcctga
     9721 atctgtcgct cgattcacag ctttatccgc aaatttccgg tcataagagc cgttttgcca
     9781 ttcgttttat gccgctggac agtgaaaacg gacaagtacc agaacgtctg gatttcgaac
     9841 tggcctgttg ctaaggagta aaaatgtcag aaactattac ggtgaattgc ccaacctgcg
     9901 ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc
     9961 agctgatcga cctcggagaa tgggctgctg aagaaaaacg aatccccagc agtggcgatc
    10021 tttccgaaag cgatgactgg agcgaagagc caaagcagtg acattctatc tgatgcgatg
    10081 ctggcctgat aaaacgcgcc agcgtcgcat caggcatcat atgcaaatgt cggatgcagc
    10141 gtgacgcctt attcgacata cacacgctta agtttcgcaa ttaccggttc attggctggc
    10201 ggaaaatcat cggcattaag accgaccagc gacatccact caccgggttg cccttcttta
    10261 ccccacggct ccccttccca gctttcgacc agccagaacc acagagttat atgcctgtcc
    10321 gggaattcat attccagttt ttcaaatagc gaaaaatgtt gtggggtaat cccgacttct
    10381 tcctgaagtt cacgtaccac tgcctgttcc ggcgtttcgc ccatttcaat tttaccgccg
    10441 gaaaactcca gtttattcgc catgtgcgca tctgctgcgc gacgcgttat aaagatttca
    10501 ttgttctcgt tgcgaataat acctaccgca atttgcagct ttttcattgt cttaaaccta
    10561 taaaaaaggc gcagaatcct gcgcctttta cttcaacagt tagcttttat tgcaggcggc
    10621 catggcactg cttgtatttt ttaccagaac cacacgggca aggatcgtta cgtcctactt
    10681 ttcgttcacc ggtttgcgcc gccagtgcag ctgcggctgc agagtcgtca tcctgatggc
    10741 taagctgctg catttgcgct aaacgctcgg cttccatacg acgctgttgt tccagctcct
    10801 caacctcttc aggcatacgc acctgaactt tgctcagcgt gctgataact tcatatttta
    10861 acgactccag catcgctgca aacatggaga acgattcacg tttgtattcc tgcttcggat
    10921 ctttctgtgc atagccacgc aggtggatac cctgacgcag atagtccatc gcagccaggt
    10981 gctctttcca cagagagtca agcgtttgca gcatgacgcc tttctcgaag tgacgcatca
    11041 tctcagcacc aaccacttct tctttacgct gatacacttc gatggactgc gccagaatgc
    11101 gctcacgcag cgtctcttca tgcagttctg gttctttatc cagccactcg gcaattggca
    11161 aatcgaggtc gaaatcgttc ttcagacgtt cctgcagccc cggaatatcc cacatttctt
    11221 ccagcgactg tggcggaatg taggcatcaa tggtcgcttt gaacacatct tcacgaatgc
    11281 tgttgatggt ttcgctcaca tcgctgacat ccagcagttc gttacgctgg gagtaaatgg
    11341 cgcgacgctg atcgttagcc acgtcatcat attccagcag ttgcttacga atgtcgaagt
    11401 tacggctttc aactttacgc tgggcgttgg caatcgcttt ggtcacccac gggtgctcaa
    11461 tggcttcgcc tggcttcata cctagtttac gcatcatgcc ggatactcgg tcggaggcaa
    11521 aaatacgcat cagcgcatct tccatcgaca gatagaaacg ggaagaacca gcatccccct
    11581 gacgaccaga acgaccgcgc agctggttat cgatacgacg ggattcgtga cgttcagtac
    11641 cgatgatatg caggccacct gctgccagta ccgcatcgtg acgtacctgc cagtcggctt
    11701 taattttttc aatttgctct gcagtcggat tttccagcgc ggcaacttct gcctgccagc
    11761 taccaccgag cacaatatcg gtaccacgac ccgccatgtt ggtcgcgata gtcaccgcag
    11821 ccggataacc tgcctgagca acaatcgccg cttcgttggc atggaatttg gcgttcagga
    11881 cgttgtgctt aataccggct ttggtcagtt catttgacac cagctccgat ttttcgatgg
    11941 agattgtacc caccagcacc ggctggcctt tcgcagtacg ttctttgata tcttcaatga
    12001 tcgcctgaat tttttccgct tcagtcatgt agaccaggtc cggcagatct ttacgaatca
    12061 ttggacggtt ggtcggaaca acgacagtat ccagcttata gatggagctg aattcgaaag
    12121 cttcggtatc agcagtacca gtcatccccg ccagtttttc atacagacgg aagtagttct
    12181 ggaaggtgat cgaagccagc gtttggtttt cgttctggat ctgcacacct tctttcgctt
    12241 ccacagcctg gtgcagacca tcagaccagc gacgcccctg catggtacga ccagtgtgtt
    12301 cgtcaacgat gataacttca ccatctttaa cgatgtagtc gacgtcacgg gtaaacagcg
    12361 catgagcgcg cagcgccgcc gttacgtggt gcatcagcat gatgttggcc ggagagtaca
    12421 gagactcccc ttcatccatg atgccttctt tcaccagcag ttcttcaatc agcaccagac
    12481 cacgttcggt caggttcacc tggcgagatt tttcgtccac cgagaagtgg ccttcgccct
    12541 ggaaggtttc ggagtcttct ttttcctgac ggatcaggtg cggaataatt ttattcacgc
    12601 gtttatacat ttccgagctg tcttctgccg ggccagaaat gatcagcggt gtacgcgctt
    12661 catcgatcag gatggagtcc acttcgtcca ccagcgcata gtgcagttta cgctgtacac
    12721 gttcttcagg actgaacgcc atgttgtcac gcaggtagtc aaagccgtat tcgttgttcg
    12781 taccgtaagt gatgtcagca gcgtaggctt cacgctttgc cggtgctggc atgcccggca
    12841 ggttgatacc gacagtcagg ccaaggaatt caaacagcgg acggttgttt tcggcgtcac
    12901 gttgcgccag gtagtcgttg acggtaacga cgtgcacgcc tttaccggtt agtgcgttca
    12961 ggtaagcagg cagcgttgcg gtcagggttt taccttcacc ggtacgcatt tccgcgatgc
    13021 agcgttcatt aagaaccata ccgccgagca gctgtacgtc gaagtggcgc atgccaaaaa
    13081 cacgcttact ggcttcacgc accacggcga aggcttccgg gatcaagttt tccagcactt
    13141 cgcctttttc cagacgcgcg cggaactctg ccgtttttcc tttcagttct tcgtcggaga
    13201 gtttttcaat ctccggttcc atggcattga tgatgttgac cactttgcgc atccggcgca
    13261 gggtgcgatc gttacgacta ccgaaaactt tagttaataa tttgattagc ataataaaat
    13321 ctcaaacgcc ccgcgttgcg gagttaataa aatgaagtaa aggtttattg ttgttagctg
    13381 aggcgttgag ggccagcacg gatgccttgc gcctggctta tccagacggg cgtgctgaat
    13441 tttgcttgtg gggtaaaatg cgcataatca atgcgataac ccttttcaga cggcgtgcct
    13501 tcctgggtca gcagcgcgct gagcgtatcc agtaatgcaa gatgttgcgc ctgaagaggc
    13561 aaagattctt cagcaacggg cagcgtttgc ggtgccattg caaaggaaag atggcggatt
    13621 accgtgcgaa tggcatgttg atgccagtaa tcaacggaat agttcgaatt cgggcggcgc
    13681 gtgttcgttt ccagcaaggc caattgacca aagttaactt tggctgaagg ctcgtggttg
    13741 cgggttgtcg cttttgcggg cgcgtttggt tcggcggcat tgctgagtgc aggcaaacct
    13801 aaactcgccg cgaccatccc taataagaga tgcggccaga agtagcgttt accaaactgt
    13861 cgccagcgcg tcagtattcc actcacgtta ttgccatccc gatcgtattc aaacgtattt
    13921 agcgcacaaa tcttatcatt aaagcctgaa gtccacagca cgaatgaaga taagttgtgc
    13981 gtaaaagtgc ttaagaaaac agcgttcgca ccgtttttta tagctcttat aaaattcaaa
    14041 aaaaacgact ggttcacctg gccggagaga gtgccagatt tgccagtcga attttatacg
    14101 acagtataaa tgtcgttatg ccagtacggc tgaaggcgct ttgaaggcca acggcagttc
    14161 tgcgtcgtcc tggaaggtca catattccca ggcttcctgt ttcgccagga cagcctgcag
    14221 cagtttgtta ttcagtgcat gaccggattt ataagcggta aatgcaccaa taatattgtg
    14281 accacacatg aacaagtcac cgatcgcatc gagcattttg tgacgcacaa attcgtcttc
    14341 aaaacgcagg ccgtcttcgt tcagtacgcg ataatcgtca acaacgatgg cacaatcgaa
    14401 gctgccgccc aggcacaaac cacgggactg cagatattcg atatcacgca tgaaaccgaa
    14461 cgtacgcgca cggctgatct ggcgcataaa cgcatcagcg gagaagttca tcgcatagcg
    14521 ctggttgctg gaatcaatag ccggatggtt aaaatcgatg gtgaaatcca gcgaaaaacc
    14581 attgtacggt ttaaattcag cccacttatc gccatcttcg acacgaacag tctctttaat
    14641 gcgaacaaat ttcttggcgc agttcaactc gtcgataccg gcgtcaagca gcaggtatac
    14701 aaacggagcg gcgctgccgt ccatgatcgg gatttccggc gcgttaactt cgataacaat
    14761 gttatcgatg cccaagcccg cgagagcagc attgaggtgc tctacggttg aaatccgtac
    14821 atcatgctcg ttgaccagac acgtacagag catggtatca cgcacagatt tggcatcggc
    14881 cgggaaatct accggtggat tcaagtcggt gcgacgatag atgaccccgg tgttggccgg
    14941 cgcagggcgt aacgtcaggg tgactttctt gccggtatgt aaaccgacac ccgtcgcctg
    15001 aacgatacgt ttaagtgtcc tttgtttgat catcgtatta tctcgccaaa ttacctatcc
    15061 aaccgaagtg tactatacat tcggcgggcc agtttagcac aaagagcctc gaaacccaaa
    15121 ttccagtcaa ttcttaatca gcttgcttac gcaggaatgc tgggatatcc agataatccg
    15181 gctctttcgc agtttgcggc gcattgtcat tcacgacttt agcaaccggc ttctgctcct
    15241 gggtcagcgg agccataccg tgctgctggt agcgatccat cactggctgc tgaacctgct
    15301 tattggtcac cagagtgatt tcaggacgtt tgtccatgcc gatacctgtc gcaacaacag
    15361 ttacgcgcag ctcgtcattc atatccgggt caagagaagt accgataacc acggtcgcgt
    15421 tgtcggaagc aaatgcacgg atggtgttac ctaccgtttc gaactcatcc agacgcaggt
    15481 cgaagcccgc tgtgatgttt accagcacgc cgcgtgcgcc agacaggtcg atatcttcca
    15541 gcagcggaga agagatagcc atttcagcag cttcttccgc acggtcttca ccgctcgcca
    15601 caccagaacc catcattgcg tagcccattt cagacattac ggtgcgtacg tctgcaaagt
    15661 ccacgttcat caaacccgga cgagtaatca gttcggcgat accttgcaca gcgcctttca
    15721 gtacatcgtt cgctgcgcca aacgcatcca gcagggagat accacggccc agaactttca
    15781 gcagtttgtc gttcgggata gtgatcagag agtccacatg cttggacagt tcagtgatcc
    15841 cctgctccgc gaatgccata cgcttcttgc cttcaaagtt gaaaggctta gtgacgacag
    15901 caacggtcag gatacccaaa tcttttgcca cttcagcgac gactggtgct gcacctgtac
    15961 cggtgccacc acccataccc gcagcaataa agaccatgtc tgcaccttcc agcgccgcac
    16021 gcagtgcatc gcgatcctca tcagccgcat tgcggccaac ttctggatta gcgccagcgc
    16081 ccagtccttt ggtgataccg ctaccgattt gaatcgtctg tccaaccgct gttttacgca
    16141 gcgcttgtgc atcggtattt accgcgaaga attcaacacc ttcaatgcgc tcacgcacca
    16201 tgtgttcaac agcattaccg ccgccgccgc cgacgccgat gactttaatc accgcgtcat
    16261 tggtaagttc cattggttca aacatagttt ctctccgatt tgtgcctgtc gcctgaggcc
    16321 gtaatcatcg tcggcctcat aaaaattaaa actcttttcg cagccaacta ttgagtcgct
    16381 tgatccacga gccaactgat gctgtaacac gtttttctac ttcagcttca ccgttaagat
    16441 gtgactcttt cccatagtga agcaatccca ccgccgtcga ataatacggc tcctgagcat
    16501 aatccgttaa accggtaatg ttcagcggcg cgccgatacg cacttgcgta tgaaacacgc
    16561 gctgagcaca ggctgcaaga ccttcgatct gcgctgcgcc accggttaat acaatgcctg
    16621 ccgccaggtg atgtttaacc ccttgttggc gaagcttttc ctgcaactgc aatatctctt
    16681 cgttgaccag attgagcagc tcggtatagc gtggctcgat cacctctgcc agggtctgac
    16741 gttgcagact ccgcggcgga cgaccgccta cgctcggcac ttccacgctc tcatcttttc
    16801 caacgatgga gcccagcgca caaccgtggc gaactttaat cgcttcggcg tcacttggcg
    16861 gtgtgccaaa ggcgtaagcg atatcactgg tcacgacatt gccagcataa ggaattacct
    16921 tcgtgtggcg caatgcccca ccggtataaa cggcgatatc cattgtacca ccaccgatat
    16981 cgacgacgca gacacccagt tcacgttcat cttccgtcaa taccgaataa cttgatgcca
    17041 gaccggcaaa tatcagttgg tcaactttca gcccacaacg ttcaaccgct ttgacgatgt
    17101 ttttcgccat atcattgtga catgtgatca ggtgcacttt tgcctgcatc cgcacgcccg
    17161 aaagtcctac cggattcttg atcccttcct gatagtcaat cgcatactct tgcgggatca
    17221 catgcagcac acgatgctca tcgcgcacac gcaccgattt cgcggtatgg acgacgtttt
    17281 ccacatcttc ttgcgtcact tcttcttcag aaataggcac cataccaatt tcattctggc
    17341 agctgatgtg cttaccagaa agcgccagat ataccgaaga tatctgacaa tctgccatca
    17401 attctgcctg gtcaatggcg cgttgtacgc acttgaccac ggattcgagg tcgttcaccc
    17461 cgcctttatc cataccacgc gacgggcaac tacccacgcc aatgatattg accataccgt
    17521 cgggcagaac ttcccctact aaagcggcaa ccttcgcggt accaatctcc agtcctacta
    17581 ccagttttct gtccgtcgcc ttgatcattg ttgttctgcc tgtgcctgat tttgttgctg
    17641 agtagattcc tctggcggca agggcgccca gcctactgcc gctccagagt cataacgcaa
    17701 atcaacgtag ctaatccgtt tgccatcggt ttgcgcctgc tgctgtaaaa ccggataaag
    17761 ttctacaaag cgagccaaac gtttcatcgt atcgccccgg ccaagattga gcttaatatc
    17821 gttattcagc gtcaactgcc aggaacgtcg cgcggtcatc gccgcttcct tcagagtaaa
    17881 tctgtccttt gccagcatct gccccatttc gcgatagccc tgcaacactt cattggcgct
    17941 gccttccggg ccatacagca ttggaagcac ctgcttgctg gtgcgatctg gcggcacgct
    18001 gaaggtgttt ccttccgcgt ctaccatatg ttgatcattc caccgcgcaa tcggcacata
    18061 ttcaaccaga tgaatcttca attcatcagg ccactgcttt ctgacgctca cctgcttaat
    18121 ccacggcagg cgttgttcta tttgcgtctg gatgatgttg acatcctggg tcataaaggt
    18181 gcctggctca cccaatgcca ggatcgactg ccggatatcg tcattacgcg tgtaatggcg
    18241 ttcaccggtc aacaccagct ttgagagcgg caggcgttgc gcatcttcca tccagcccaa
    18301 cacgacccag ccgctcacca acactgtcgt taaaacggtc agcaggaaaa ggatccccgc
    18361 cagacgcgtt ccattattgc ggcgagaaga aacctcttct tcgctatttc gcgtgttcag
    18421 agcagcctgc gacatattag tccgccagtt ccagaattcg tactaccaac tgcgagaagc
    18481 tcatacctgc ctgacgtgcc gccatcggca ccagactgtg gctggtcata cccggtgagg
    18541 tattggcttc cagcagataa aactgtccat cgctgtccag cataacgtca atacgtcccc
    18601 atcctttgca acctaacgtc gtccatgctt ttagcactaa tgcctgcaaa ttggcctctt
    18661 gtgacgcttc cagacctgcg gggcagaaat actgtgtctc atcagagaga tacttcgcct
    18721 cataatcata gaaggttccg gccggttgaa tacgtattga cggtaaaatt tcttcaccga
    18781 gtatcgcaac cgtgaactcc ggcccactta gccatttttc aatcaatact tcttcatcgt
    18841 gctgaaatgc caatcttaat gcatcttgta gagcattttc tgctactact tttgacattc
    18901 ccacactgga accttcgcgg ctcggcttaa cgattaccgg caaacccaga gaagaaattt
    18961 ctgctaactg cttatcgtta aggccttttt caaactctgc gcgggttaat gctacccacg
    19021 gcgcgaccgg taaaccggca ctctgccaca gaagtttgct gcgtagttta tccattgaaa
    19081 gcgcagatgc catcactccg ctgccggtat aaggcaagcc catcagctcg agcatgccct
    19141 gcagcgtacc atcttcaccg ccgcgaccgt gtagcgcgat aaacactttc tgaaaaccca
    19201 tcgacttcag ttgcgtcacg tcgacttctt tcgggtcgac aggatacgcg tcaataccgc
    19261 cttcacgcag cccggctaac accgctgcgc cagaattcag agaaacttcc cgctcagcgg
    19321 aggtcccgcc caacaggacc gcgattttat cagtcatgtt gttcttcctc cggagtttgc
    19381 ggcttcagtt tgatttcagc taaagaacga gcaatttttc cgatattacc agccccctga
    19441 acgagaatca ggtcgttacc ggttaatacc ggtgccagca tctcggctac ctgcgccggg
    19501 tccggcacca gaatgggatc aattttccca cgtccacgaa ttgtgcgaca cagcgaacgg
    19561 ctgtccgctc ccggaattgg cgcttcgcca gccggataca cttccagcat caacagggta
    19621 tcaacctgcg tcagcacatt ggcgaaatca tcatacaggt cgcgcgtacg ggtaaaacgg
    19681 tgcggctgaa acagcattac caggttttta tccggccagc ctgcacgcgc cgctttaata
    19741 gtggcgtcca cttccgtcgg gtggtggccg tagtcatcga ccagcattgc cgtaccgctt
    19801 ttaccattca ctggctccag cgggaattca ccgaggaaat cgaaacggcg ccccgtcccc
    19861 tggaagctct ccagcgcacg caaaatagcc tcgtcgtcaa ttccctcttc cgtagcaacc
    19921 gcaaccgcag ctgcggcgtt cagcgcgtta tgacgacctg gcgcattcag ggtgacgcgc
    19981 atcggctctt tgtcctggcg cagcagcgta aagtgcccct gcgggccaat ctgctgataa
    20041 tcttctacac gcacgtcggc atcttcgctg aagccgtaag tcgtggtctg acgccccaca
    20101 cgcggtaaca attcgcggat caccggatca tcaacacaca tcaccgcgcg accgtaaaac
    20161 ggcaggttgt gcagaaaatt aataaaagtc tgttttaaat tctcaaagtc gccctggtag
    20221 gtatccatgt ggtcggcttc gatattggtg acaatcgcca ccatcggttg cagatgcagg
    20281 aacgatgcat cactctcatc tgcttcggca atcaggtacc gaccatgccc caaacgcgca
    20341 tgaacccccg ccgcttttac cagcccgccg ttaacgaagg ttgggtcgag ccccgcttct
    20401 gcgtagatgc tggaaaccat cgcggtggtt gtcgttttgc cgtgcgttcc ggcaatggcg
    20461 atgccatgac gaaaacgcat taactcagcc agcatttcgg cacgacggat caccggaata
    20521 cgcgcttcat gagctgcgac aatttccggg ttatcggcag aaatcgcgct ggaaacaacg
    20581 accacgctgg catcacgtac gttttccggg cgatggttga aataaatcgt cgctcccaga
    20641 ttcattaact gctgcgtgac cggatttggc gctaaatcgg aaccactgat ctgataacct
    20701 tcattggcca gaacttcggc aataccgccc ataccggcac caccgatgcc gacaaaatgt
    20761 atgtgccgaa cgcgacgcat ttcgggcacg atggaacgca gttttgccaa ttgttgtgta
    20821 ttcattcttt acgccattaa cttcttaaat tcatacgatg caaaaggcat cgctacaatt
    20881 acgcccgggc agcccggctc acttcatttg ccactcgctc ggtggcatcc ggaatggatg
    20941 cagcgcgggc gcgttctgcc atggttaata aggtttctcg cgaccacccg gccagggtgt
    21001 tcgcgacagc atccacgcta agctgtgact gctcgataat tttggctgcg cccgcttttt
    21061 ccagcggtag cgcattccag tattgctgac ggtctttatg ttgaaacggc acaaacaacg
    21121 ccggtagtcc tgccgcggcg atttcactca ccgttaacgc accggagcgg caaacgacga
    21181 catccgccca cgcatacgcc gccgccatat catcaataaa ttccgtcact ttatgctgcg
    21241 gttgccccac ttcggcatac gcctgttcaa cggattgttg cgaacctttg ccgctctgat
    21301 gccagatagt gactgaatca cccagtttcg cagcaacctg cggcattgtc tgattaagaa
    21361 tgcgtgcgcc ctgggaacca cccactacca acacacgaac cggaccttca cgtccagcca
    21421 aacgttgctg cggcaacggc agcgccagca catcggtacg caccgggtta cccaccactt
    21481 ccgcattagg gaaagcacct ggaaacgcct gcatcacttt ggtagcaatc ttcgccagcc
    21541 atttattggt taagcccgca ataccgtttt gttcatgaag tacaaccgga atgcctaacg
    21601 accacgcggc cagaccacct ggacctgata catagccgcc cataccgagc accacgtcag
    21661 gtttgtacgc tttcataatc gcccgcgcct gacgccaggc gttgaagata cgcagcggcg
    21721 cagctatcag tgcttttata ccttttccac gcagaccaga gatacgaatg aaatcaattt
    21781 cgatgccatg ttttggcact aagtccgctt ccatacggtc ggcagtcccc agccagcgaa
    21841 cttgccaacc ctgagccatt agatggtgcg caaccgccag ccccgggaat acatgtccac
    21901 cggttccgcc tgccatcacc attaatcgct ttccttgacc actcatcgtg aacctcgtac
    21961 aaacgcctgc gctttctcca gacgcgtttc ataatcaata cgcaacagca tcatgatggc
    22021 tgtcgacata atcagtaagc tcgaaccacc gtaactgatc agcggcaatg tcagaccttt
    22081 ggtcggtaac atccccgccg ccgcgcctac gttaaccagc gcctgaaaac taaaccagat
    22141 accaatagaa caggcgagaa aaccggaaaa acggtggtca atttctaatg ctttgcggcc
    22201 aatcgacatc gcgcgaaaag cgacgaagaa taccattaaa agtgccagca ccacaccgac
    22261 atatcccagt tcttcgccga taatggcgaa aataaagtca gtgtgcgctt ccggcagata
    22321 ctccagtttt tgtaccgagt tacctaaacc ttgtccccac agttcaccgc gaccaaacgc
    22381 catcaacgat tgcgttaact gatagccgct gccaaaggga tcttcccacg gattccagaa
    22441 tgcggtaaca cggcggatac ggtacggttc ggcgaggatc agcaacacaa ccgctgaaat
    22501 gcccataccg ataatggcaa tgaactgcca caatttcgct cccgccagga acaacatcgc
    22561 cagcgtagtc acaaacaaca ccaccaccgt accaaggtct ggctgtgcca gcagtaacac
    22621 agccaacacc agaattacgc ccatcggttt cagaaagccg cgcaggttat tacgtacttc
    22681 gtcgccttta cgcaccagat agttggcgat atagcaaaac agcgacagtt ttgtcagctc
    22741 cgcaggctgg atacgcagca aaccgagatc gatccaacgc gatgcccctt taaccgagct
    22801 acccactacc agaacgatca tcagcaggat gatagatcca agcagcatcg tggcactgta
    22861 gcgttgccag aactccatcg gcagacgcag cgtaatgatc gccagaatga atgccaggat
    22921 cagatagacg ccatcacgct tcgcgaagaa gaacggatcg ttggttaagc gttgccctat
    22981 gggcattgac gccgaggtca ccatgataaa gccaatcgcc gcgaggccga aggtcagcca
    23041 cagtaaggtg cgatcgtaca tgatcaggct gtcggtatct ttttcccgcg agcccatcac
    23101 ccagcccttt agcgccgtgg agatccagac cagaatactg aatcctggca ggcgcggcat
    23161 tttcaggcga gggagagata aacgcatcaa cctaactcct tcgccagacg ggcaaactca
    23221 ttgcctcgtt gttcaaagtt cttgaattga tcaaggctgg cacaggccgg ggagagcaga
    23281 accatatcgc ccggctgaac acgcggagca agcaagcgca tcgcctgttc catggtttcg
    23341 gtttgttctg ccacttccgg gcgtagcgcc gccagctgcg cgccgtcccg accgaaacaa
    23401 tacagacgta cattatcgcc attcaggtaa cgcgccagtg ggctaaagtc cgccgattta
    23461 ccatcgccac ccagcaacaa atgcagcgtg ccgtctacat gcaggccatt cagcgccgct
    23521 tccgtactgc cgacgttggt cgcttttgaa tcgttaaccc agcgcacacc gttatgctcc
    23581 agcacaactt caaagcgatg cggcagacca gtgaatgtgg ttaacgcttt caggctgctg
    23641 gcacgcggta accctgcggc atctgccagc gccagcgccg ccagcgcatt ggtgtagtta
    23701 tgctgcccgg aaagtttcat ttctttcacg ttcagcactt tctcgccttt cacccgcagc
    23761 caggtttcgc cctgctgatg attcaggtga tagtcgccca tgttgacgcc aaagctgacg
    23821 cagcgttcat ccgcaccgcg aatcggcatt gttaaggcat catcagcatt aaccacgcaa
    23881 acttttgcgt tttcgtaaat gcgcagtttt gctgcacgat actgttgtaa accaaacgga
    23941 tagcgatcca tatgatcttc agtcacgttc agaatggtcg ccgccaccgc ttgtaagctg
    24001 gaagtggttt ccagctggaa actcgacagt tccagcacgt acagttcgca ctcatcatcc
    24061 aacagcatca acgcaggcag gccaatatta ccacccacac caacgttaac ccccgccgct
    24121 ttcgccattt cacccactag cgtggtgacc gtgcttttgc cgttagaacc ggtgatcgcc
    24181 acaatcggtg cttgtgcttc acgacagaac agctcgatat cgccaacgat ttcgattccg
    24241 gcatcagcgg cagcgcttaa ggatggatgc gccagtgcaa taccgggact ggcgacaatc
    24301 agatctgccg ccatcagcca ttcatcattc agaccgcccg tgtggcgttc tacggcttcg
    24361 ggtaatttat ccaggccagg cggtgtcata cgcgtatcca taacgcgcgg cgtcacaccg
    24421 cgagcgagga aaaagtccac gcaggaaagc ccggtgaggc ccaggccgat aatgacgaca
    24481 tttttaccct ggtaatcagc catgattaac gtaccttcag cgttgccaga ccaatcagga
    24541 ccagcatcag cgaaataatc cagaaacgca caatgacgcg cggttccggc cagcctttca
    24601 gttcatagtg gtgatgaatc ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg
    24661 agccgacctg caggatgacg gaaagcgttt ctaccacgaa cacgcccccc ataatcacca
    24721 gcaggaattc ctgacgcagc agtacggcga taatacctaa cgcaccacct aacgccagcg
    24781 aacctacatc gcccataaag acctgcgccg gataggtgtt aaaccacagg aagcccagcc
    24841 ctgccccgac tatcgcggta cagacaatga ccagttcccc ggcgtgtcgt agatacggta
    24901 tgtgtaaata gctggcaaag ttcatgttgc cggtcgccca cgccaccagc gcaaaaccac
    24961 cggcgacaaa tacggtcggc ataattgcca gaccgtcgag accatcggtc aggtttaccg
    25021 cgttgccagt acccacaatg acaaagtaag ccagcagaat gtagaacagc cccagttgcg
    25081 gcatcacatc tttaaagaac ggaaccacca actgcgttgc gggcgtgtct ttgccggcaa
    25141 ggtacagcgc gaaggcaaca cccagtgcaa tgaccgacat ccagaaatac ttccaacggg
    25201 caatcaggcc cttggtgtct ttacgcacca ctttgcgata atcatcaaca aagccaataa
    25261 caccgtaacc taccagcacc accagtacgc accagacgta cggattggac gggtaagccc
    25321 acagcagcac ggagatcaca atcgccgtca ggatcattat cccgcccatg gtcggtgtac
    25381 cgcgcttgct gaagtgtgat tcaggaccgt cgttacgcac cacctgacca aaggaaagtt
    25441 tttgcaaatg agcaatcata cgcgggccca tccacaatga gatgaacagc gcggtcagca
    25501 ggctgacgat ggcgcgaaac gtcagatagg aaaagacgtt aaagccggaa taatatttga
    25561 ccaaatgttc ggccagccaa actaacatgt cccattctcc tgtaaagcgc gtactacctc
    25621 ttccatggca gcactacgtg aacccttaac taaaatcgta attacctgtt gctcagcaat
    25681 cagtgattta agacgcgcga taagcgcagt tttatcggaa aaatgttcgc caacgccgct
    25741 ggcggtgctg atagcatggc tttgtttacc cacacttaac acgcagtcaa taccagcagc
    25801 ttttgccgct tcgcccacct gtacatggca ggcttcgctt tcagcgccca gttccgccat
    25861 atcgcccacc accagcacgc ggtagcccgg catttcagcc agaacctgaa cagcggcggt
    25921 cattgaaccg acattggcgt tgtaggagtc gtcgagcagc aactggtttt ctgccagttt
    25981 tatggggaac agacggcctg gaacagcttt cagatttacc agccccgctt tgatagcatc
    26041 aagcgttgcg cccacagaca tggagagcgc agcggctgcc agcgcattcg caatattgtg
    26101 acgccccggc aacggcagca gaacatcgac gctacctgta ggggtttgca gggtaaattc
    26161 cgtaccgtgc gaggttacat ggatattggt ggcggtgaaa tcgctgttgg cggcattagg
    26221 tgagaaacgc cacactttgc gtgagccaat tacgctctgc cagttcaacc agtcgttgtt
    26281 gtcggcgttc ataatggcga taccgttttc cggcaggccg ctaaagattt cacctttcgc
    26341 tttcgcgaca cccgcaagcg agccaaaacc ttccagatgc gccgctgcca ggttgttgac
    26401 cagcgccgct tccgggcgag tcagactcac agtccaggca atttcaccct gatggttcgc
    26461 gccaagttca ataactgcgt aatcgtattc cggcgttaag cgcaacagcg tcatcggtac
    26521 accgatgtcg ttattgagat tgcctgccgt ataaagtgtg ttgccgcact ggcttaaaat
    26581 tgccgccgtc atctctttaa cggaggtttt gccggaggaa cctgtcagag caaccacgcg
    26641 cgccggaact tgctggcgaa cccatgcagc cagttcacca aacgccagac gcgtatcctt
    26701 gacgattaac tgcggcagat caatatccag cggacggcta accagtagtg cgcctgcgcc
    26761 gccagctttc gcctggtcgg caaaatcatg agcatcgaaa cgttcgcctt tcagggcaac
    26821 aaacaggcag cccggcgtca gttttcgcgt gtcagtggtt acagcatcaa gggtaatatc
    26881 tgcaccttgc agttcaccgt tgagaatatc ggtaagttgg ctaagggtta cgctaatcat
    26941 gcaatcaccc ccagcagacg cgccaccgtg acgcgatcgg agtagtccag acgctgattg
    27001 ccaacaatct ggtagtcctc atggcctttg cccgcgacca gtaccacatc attctcttta
    27061 gcctgcataa cggcgcaagt caccgcttca gcacggcctt ccatcacttt ggcatatccg
    27121 gcatctaaca ttcccgccag aatatcgttg atgatggcac gaggctcttc ggtacgcggg
    27181 ttatcgtccg tcaccaccac cacgtcagca aactcttcag caattgcgcc catcagtgga
    27241 cgtttgcctt tatcacgatc gccaccgcag ccaaagacgc accacagctt gcccgcacag
    27301 tgcaggcgcg ccgcctgtaa ggctttttcc agtgcatccg gcgtatgcgc gtaatccacc
    27361 accaccgtcg gtttgcctgg tgcagtgaac acttccatac gtccgcaaac cggttgcaga
    27421 cgcgcggcgg ttttcagcaa atcagccagc ggatagccga gtgccagcag tgtcgccagc
    27481 gccagcagca ggttgctgac gttgaatgcg cccatcaggc gactttcaat ttccccatcg
    27541 ccccaactcg agctaaagca aatggtcgca ccgctgtcgt gatagttcac gtcggtcgct
    27601 ttcaaccagc gtccgtgaca gttcggattg atatgatctt ccattgatac cgcaaccgcg
    27661 tccggcagtt ttgccagcca gcggcggccc acttcatcgt cggcgttaat aatcgcctga
    27721 ccgcaatgat gtgcagaata aagcagccat ttcgcggctt cgtagtgttc catatcacca
    27781 tgataatcaa ggtgatcgcg gcttaagttg gtaaagaccg acgccgcaaa tttcaatgcc
    27841 gccacacggt gttgaaccag cccgtgggat gaaacttcca ttgcgcaaaa agtcgcgccc
    27901 tgatccacca gccccgccag ctcatgctga acatcgactg ccgaaccggt tgtattttct
    27961 gtcgggatca ctttccccag caggccgtta ccaacggtgc ccattaccgc gctggtttcg
    28021 ccaagcagtt gactccactg cgccaacagc tgggtagtcg tggttttgcc gttggtgccc
    28081 gttacgccca cgagacgtaa attgtcagag ggttcatggt aaaagcggcc cgccagtgca
    28141 gataaacgct cgttgagctg gctgagatag atgaccggta cgccgtgcat ttcacggatt
    28201 tcaccgtcgg tcgcctcatc tttcgcctct gcaataatgg cagccacacc ttgcgctatc
    28261 gcctgcggga tatatcgacg cccgtccgcc tgatgaccta ctacagctac aaagagatcg
    28321 cccgccgcag ccacacggct gtcgagtgtc atctctcgca gtgctcgcga aggtgcgtct
    28381 ggcacccacg gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtac cctcgccttg
    28441 attaatcaca aattcatttt tatcgcctgt tgtcagcgca tccggctcga tgttcatggt
    28501 acgcaatacg ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc cgccgtagta
    28561 tttacccgcc tgcggatcgt tgataacaac aaccagcgcg aagcgcggct gactcgcagg
    28621 cgcaacgcct gcggtataag caatatattt attgatgtag cgaccatccg gcccgacctt
    28681 tttcgcggta ccggttttaa tggcgatacg atagccttta atcgccgcct tcacgccgcc
    28741 gccgcccggt agcgccacgc tttccatcat atgcaccacg gtgcgaacaa tggattccgg
    28801 gaagacacgt tcaccgggaa ccggggggtc aactttggta atcgacagtg ggcgataaat
    28861 gccgtagctg ccgatagttg cgtagactcg cgctaactgt aacggtgtta ccattagccc
    28921 gtagccgaaa gagaaggtgg ccctctctat gtcagaccac cgttgttttt gaggatataa
    28981 gccactgcgt tctccgacca accccaaatt ggtcgctttt cccagcccaa aacgtgagta
    29041 agtatctact aacgctgagg acggcatcgc taacgccagt ttggaaacac cgacgttact
    29101 cgacttctgt aataccccgg tcagggttaa ttcgctgtag cgtgccacgt ctttgatttc
    29161 gtggccgtta attcgataag gaacggtatt gagtaccgag ttttcccgca ccacgccacg
    29221 ttgcaacgcg gtcatcacca ccatcggttt aaccgttgag cccggttcaa acacgtcggt
    29281 gatggtacgg ttacgcatcg cttctttcgg cgtgccgctc agattatttg ggttgtatga
    29341 cgggctgtta gccatcgcca gcacttcacc ggtgttgaca tccaccagca cggcgctacc
    29401 agattcagcc ttgttaaagg ccactgcgtt gttcagttcg cgataaacca gcgcctgcag
    29461 gcgttcatca atactcagcg tcaggttgtg cgctgcctgg ctgtcagtag aagaaatatc
    29521 ttcaattacg cgaccatagc ggtctttacg cacaatgcgc tcacccggct gcccggtaag
    29581 ccatttatcg aaactcttct cgaccccctc aatcccttgg ctatcgacgt tagtaaagcc
    29641 gatgaggtga gcagtcactt cgccggatgg ataataacgg cgagattctt cacgcagatg
    29701 aattcccggc agtttcagtt ttttgatgta gtccgccatg tcagggttta cctgacgcgc
    29761 cagataaata aagcgccctt tcgggttggc gttaatgcgg gctgagagct gatccagcgg
    29821 aatattgagc gcgttagcca gcgccttcca gcggtcaccg acgctgatac cgccagcgtc
    29881 atgcacttct ttcgggtcag cccaaatcgc ttttactggc acactcaccg ctaacgggcg
    29941 accagaacgg tcagtaatca tgccgcggga ggtggaaact tgctgaacgc gaagagaacg
    30001 catgtcgccc tctttcacca gcatatccgg ggagataact tgtaaccacg ctacgcgtcc
    30061 gagcagaaaa gccagcgcca ggagaataca gccgcataac aacgcaaaac gccaactgat
    30121 aaagttggca tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg
    30181 tcgcgtttat ccttattttt gcactacgat attttcttgt gacggatcaa catgctgcat
    30241 ttgcagcttt tccgtggcta tcctttccac ccggctatgg tcgccgagcg cattctcttc
    30301 aaggatcagg ttgcgccatt caatgtctaa agcatctcgc tccagcacca gttgttcgcg
    30361 ctgagcggtc agtaaacggg tatggtgcgc cgtggttacc acggtcaccg ccgtcaaaat
    30421 aatgcaaatg aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt caccgataac
    30481 gccaggcaag gcatggcgct cgtggcttcc catcgatcct ttaactttgc ttagagcttc
    30541 tgtcactctg ctgatcatgc gttcgtcctc tctgcaatac gcagaactga actacgggca
    30601 cgagggttct cagccacctc ttcttcgccc ggcatcaact tacctagtgc tcgcaactga
    30661 cgccccccca gttttttgag ctgctcttct gtcatcggta accctgccgg aacttgcgga
    30721 ccgcggctgt tttcacgcat aaaacgtttc acaatgcggt cttcaagtga gtggaagctg
    30781 atgatcgaaa gccgcccccc cggggccagc acattgagcg agctttttag cgcctgctct
    30841 atctcctcca gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg ggtcgcggga
    30901 tgtttaaact tatctttcac cggcgttgcg gcagccacga cttccgccag ttctttggtg
    30961 cgggtcatcg gctgttcgcg gttacgctcg acaatggcgc gagcaatgcg tttggcaaaa
    31021 cgctcttcac cataggtttt caatacccag gcgatatcgg cttcttctgc ggtttgtagc
    31081 cattcagcgg ctgactgccc acgggttggg tccatacgca tgtccagcgg accatcgcgc
    31141 ataaaggaaa agccgcgttc agcatcatca agttgcggtg aagagacgcc aagatcgagg
    31201 agaatgccgt cgatcttgcc gataagatcg cgctcggcaa catattcgcc cagcgcagag
    31261 aaaggtccgt ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc
    31321 tgcgggtcgc gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga gaggatcaga
    31381 cgtgagtgac caccgcgacc aaaagtccca tcaatgtaga tgccatcagg acggatattg
    31441 aggccattaa cggcttcatc cagcagcacc gtagtatgtt tatagttttc catcatttta
    31501 tagagacaag tcctgcagtc gctccgataa gtctccggta gccaactgct ctgcgtcgat
    31561 atcttccttg acctgttgat gccaggttgt ttcatcccac agctcaaact tgttgaactg
    31621 tccaaccagc atcacttctt ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa
    31681 caatcgacct gcgccatcca tctgacattc gctggcatga cctaacagta ggcgctgcac
    31741 acggcgctca accgggttca tgctcgacag acgcgataat ttttgctcga taatttccca
    31801 ttcaggcagg gggtaaagca gcaggcacgg gtgatgaatg tcaatggtgc aaaccatttg
    31861 accggcagcg ttctcaagca gctgttcccg ataacgggta ggcactgata agcgcccttt
    31921 gctgtcgaga ttgactaacg ttgctccccg gaacatgcca gcctcacccc ttattcccac
    31981 tttgccccac aatttcccac taaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc
    32041 agtcacaagg cataccgagg taaaaaaccc gatgtttacg gctaatatca gcgttgttta
    32101 ttttacgtcc aacgtgcgaa gcaaattaac gttatgaata gctgtaagaa aaaatccctc
    32161 atcgtcatca ccgttttaaa atgactcact attttccaca gcaaatataa agtgtcagtt
    32221 tgcgacgcga gcggcatttt aggacatatc ttctctggtt aacagtccct gttgcgtctg
    32281 tgtggcgctt gatgccagca ggtttgcgca aaaacaaaga aaagtgtttg ttaaatcggc
    32341 aattcatctc tgcatgtaac aaaataatgc aaaaacgcgc tgtcaatatc atttaattat
    32401 tgtgcatttc agcaggtgag ttaattaacg ggcgggttta aaagcattta tcaataatag
    32461 ccaggaatag tcttatttac tttaaggata ttgatgtcca ttcccgtact caacgcgcca
    32521 gagagaaatt cacctggcgc gtacttttgt tcgcggttta gctacggctg agcacgccgc
    32581 ggcgatagag attacgttta atgcgcgtta aaccaggttt tggcttacgc ggttcgtcca
    32641 ggctcgccag gacaatctcc agcacacgtt ctgcgacatc gcggtgacgt tgagccactg
    32701 ccagcaccgg gcactgtaag aagtcgagca gttcgttatc gccaaaggtg gcaattgcca
    32761 ggtcagaagg cagtttgccg tcgcgacgta gcgtgacatc catcacccct tgcaacaacg
    32821 caaacgacgt tgtgaacagc gcctgcggca tcggatgcgt ttccagccat ttttcgaata
    32881 actgggcagc tgcctcccgc tcatagctgt tggcataaag gaaatgcact tcgcgcggat
    32941 catctttcca ggcagtacgg aagccttgtt cacgcaggaa gctgacagaa agctccggca
    33001 gcgcgccaag ataaagcacc gtctcggcgg gaaacttacg taactcttcc gccagcattt
    33061 cggcatcatc ctgatcggca ccaaccacgc tggtgaagtg ttcacgatcg agggcgcggt
    33121 ccagcgcgac aatcgggaac gggtcgttag cccagcgttg ataaaaagga tgctcaggtg
    33181 gcaacgacgt cgaaacaata atggcatcaa cctgacgctg taaaaggtgc tcaatacagc
    33241 gcatttcgtt gtcgggctga tcttccgagc aggcaatcag cagttgataa ccccgttgcc
    33301 gcgcctggcg ttcaagatag ttagcgatgc gggtatagct ggtgttctcc agatcgggga
    33361 tcacaagacc aatagaacgt gtgcgtccag cacgaagccc agctgccacg gcgttcgggt
    33421 ggtaattgtg ctcacgcacc acagccatga ctttttcaac ggttttgtcg ctcacacggt
    33481 attgcttcgc tttgccgtta ataacatagc ttgcagtggt ccgcgacact cccgccagcc
    33541 gagcgatttc atccagtttc acaattgccc cttgcgtaaa atgtaaaaac catagccatt
    33601 gtactggtat gggttaaatt cattaacatc taagcgcaga aaattaactg cggcaactgc
    33661 ttttattcca ggttcccact gatttcgcaa aaaaagccca acgtgacatg ttgggctgca
    33721 aattgcgcat tgagatcatt ctcaacgcat tattttatcg ccgcgcgaaa gtccgaccac
    33781 accagagcga gcaacttcca caattttcgc cacatcgcga atcgatgcta aaaatgcatc
    33841 aagcttatcg ctggtgcctg ctaattgaac ggtataaagc gagggtgtga catcgataat
    33901 ttgcccacgg aatatttccg tattacgttt cacttcgtca cgcccgtaac cgctggcctg
    33961 aattttcacc agcatgattt cccgctcaac gtgcgcgccc tgccccaact cgctcacgcg
    34021 caagacatcg accagcttgt gtaattgctt ttcgatttgc tcaagcactt tttcatcgcc
    34081 cacggtctgg atggtcatac gcgataatgt cggatcgtcg gttggcgcaa cggtcaggct
    34141 ttcaatgttg tagccacgct gggaaaaaag gccaatcacg cgggataacg cgcctgattc
    34201 attttcgagt aagactgata atatccggcg cataatcagg ttctctccgt tttgcttaac
    34261 cacatttcat ccattccgcc cccgcgaatc tgcatcgggt agacgtgctc actgccatcg
    34321 acggtaacat caacaaacac caggcgatta ttgcgcacct gttccagcgc ctcgctaagt
    34381 ttgctttcca gttcttgcgg atgagagatc tggatcccga cgtgaccata ggcttccgcc
    34441 agacggacga agtctggtaa ggactgcata taagattgtg aatgacggcc ggaatagatc
    34501 atgtcctgcc actgcttcac catccccaga tagcgattat tgagattcac caccagtacg
    34561 ggcaactcgt attgcaaggc ggtagacagt tcctggatgt tcatctgaat actgccgtca
    34621 ccagtgacgc aaaccacggt ttcttctggc aacgccattt tgacgcccag tgccgcaggt
    34681 aaaccaaaac ccatcgtgcc gaggccgcca gagttgatcc agcgacgcgg tttgtcgaat
    34741 ggataataaa gcgcagcaaa catctggtgc tgcccgacat cggacgtcac gtaagcgtcc
    34801 ccattagtca accgccaaag agtctcgatc accgcctgcg gtttaatctt ttcactgtga
    34861 gtgtcatatt tcaggcactg acgagcgcgc cactgttcaa tctgctgcca ccagtcgcgg
    34921 atctcatcca gcggttgatg ggcggattct tgcgacagga gttcaagcat ttgttctagg
    34981 acctggcgag catcccccac aatcgggata tcagcagtca cggttttaga aattgaggtt
    35041 ggatcaatat cgatatgcag aacagtggca tttgggcagt attttgccag attgttcgtc
    35101 gttcggtcat caaaacgtgc cccgacggcg aaaatcacat ccgcgttatg catcgtcata
    35161 ttggcttcgt aggtaccgtg cattcccaac atgcccagtg cctgacgatg cgttgccgga
    35221 aacgccccca gccccatcaa agaggaaaca acgggcagat tcaacgcctc caccgcttct
    35281 ttcaactgca gatggcagcc cgccgtgatt gccccaccgc ctacgtagac aaccggtttt
    35341 tttgccgcca ccagcgtttg cagagcgcgc ttaatttgcc ctttatgccc ggaggtcgtg
    35401 ggattgtaag aacgcatact gaccgactcc ggccagacat agggtaattt gttcgccgga
    35461 tttagaatat ctttcggtaa atcaacgacc acgggtccag gacgaccact tgccgctagc
    35521 cagaaagcct ttttcagcac ctgcggaatg tcttccgttt gcttaaccag aaaactgtgt
    35581 ttaaccaccg ggcgagaaat ccccaccatg tcgcactcct gaaaggcatc ataacctatc
    35641 aacgaagtcg ctacctgccc ggaaaggaca actaatggaa tggaatccat ataagcggtg
    35701 gcaatgccag taatcgcatt ggtcgcccct ggacccgacg ttaccagcac gacgccgatt
    35761 tccccggtcg cgcgcgccag accatcggcc atatgcaccg ccgcctgctc atggcgaact
    35821 aaaacatgat cgataccacc cacggtatgc aatgcatcgt aaatatcaag gaccgcgcct
    35881 ccaggataac cgaatacttg tttaacgccc tgatcgataa gcgatcggac gaccatctcg
    35941 gctccagaca acatctccat ggcctgcctc actgtttgac ggaaaaaatg tgtaaaacac
    36001 attaaccgtc cgatcttgct taagcaattg gcagacagag acataaacac ggggtaataa
    36061 gaataaacag gaggaaaggt gaaaagaaac gataaaatcc tccattacag aggatgaaat
    36121 tgataataaa tcggctgaat cctacaactt acagcaattt ctctattttt ttaattaatc
    36181 ctaaaataga gagcacaaat attcagaata aaattctaca ttcaataaaa aatgcagaat
    36241 aaaccagaca ttgatgtctg acctattctg cattcagtta gcgtttgcaa attgagacta
    36301 attgctcttc catccactga tggcctttat cgcgcccggc agcttcatgc caggagagat
    36361 aacaggttct gctgttttgt tttaacggca gcggtaatac ctgtaactct aaggattcag
    36421 cgaactcttc agccaaccaa cgcggcgcaa tagcaaccag atgcgtttgc gacaccacgc
    36481 taagtacgct catcattgcc atgccctgat acgcgatact ggcttgctta tctaccgtgt
    36541 cataccaggt ttgactaaat gacgcgaaac gatcgagcga aaccgccgca tgttgttcgt
    36601 tataaacatc atgtttcagt aacgggcctt taattgttgg atgatttttg ctggctacca
    36661 acaccatttc atctttaaat aatggcacgc tggtaaattc aggacgatgg aagtcttcat
    36721 aactaatcac aaactccgtt tcctgataac gcagctgatg ttcagtgttc tgatttaatg
    36781 aagacttgaa cataacatgt atatttggcg caatctgctc aatgtgatta taaatctgcg
    36841 aggtcagaat gctgtctaac gggctgcaga cacaaagatg aaatacacgt tcactactgg
    36901 caggctcaaa gcctgaacct ggtaattcat tttgtactaa ttgcaatgcc tgacgaactg
    36961 agccaaaaag ttgaaatgcg cgagcagtcg gttgaatacc acgaccataa cgaacaaaaa
    37021 gctcgtcatt aaacatcacc ttcaggcgtg caacagcgtt actgaccgca ggttgcgaca
    37081 ttcccagagc atgagcggca cgggtgatgt tttgctcctg catcacggca tcgaaaacgg
    37141 ttaataagtt gagatcgacc atgcgtagct gtggcttgct taactccgcc gtctctggat
    37201 gatctgtttg tacctctggc atacttaact ccactgtcac acttaactcc ctttccct
//
LOCUS       DACADF010000027        37175 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.027, whole genome shotgun sequence.
ACCESSION   DACADF010000027 DACADF010000000
VERSION     DACADF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.027"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..156)
                     /locus_tag="ISB30_001342"
     CDS             complement(<1..156)
                     /locus_tag="ISB30_001342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988992.1"
                     /translation="MSELPFNEVNGVWKQLAAGYASTQGESFTGHGGSPVAVEEAEHF
                     FVHFDQKG"
     gene            155..607
                     /gene="radC"
                     /locus_tag="ISB30_001343"
     CDS             155..607
                     /gene="radC"
                     /locus_tag="ISB30_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019083243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP1988993.1"
                     /translation="MTMFTQRETRILDQARDIISRYYQRGVQLCSPDDVRRCVMVELA
                     PLEHEEFGIILLDNHNQLLHREILFRGTLNSVSVHPREVIKRVLKHNAAAVILVHNHP
                     SGEPEPSRCDIQLTKKLQELLEILDVRLLDHFIVAGTETVSMAERGLV"
     gene            654..1583
                     /locus_tag="ISB30_001344"
     CDS             654..1583
                     /locus_tag="ISB30_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019083242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP1988994.1"
                     /translation="MSRTALRQVVPVVQTTVFDAYIVGLAPSGRRGITSLLNRSASIL
                     KHGADAADYPWEQLNYAAVAKVRAALLDDGYAVSSVNMALSALRGVAQTAFNLNYMDA
                     ETLARIRSVKRVSGDVQRKGRALGRQEIRVLIQAAKQHPQSVRRCRDVAIVLMLCGTG
                     LRAGELVKLERRDYDNGILTVRQGKGRKYREIHVADAVDKAIRAWLKVGADEADSALF
                     RRIQRSGKVASQPLTTTGLTGILAELQQTAGIARFTPHDMRRTFITRLLEQGVDINTV
                     RQLAGHSDISTTTRYDCRGDTMKISASKRIRCF"
     gene            complement(1570..2184)
                     /locus_tag="ISB30_001345"
     CDS             complement(1570..2184)
                     /locus_tag="ISB30_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988995.1"
                     /translation="MIGNQSNKADARCHRERKSSNEVFEFLEKKLYLGRDTRQKLALI
                     ARKLTGAGFSPDKFAAEKAADVLSACVNHMFNDLLNEGEPNGIYRPEEHPMIVPARSP
                     KALEIYTVYQLVKGRFDKLHTGESDREKYASVAEKLNEYGIWKPRLKRLSDGKEWARE
                     DVAWMLQPENINAQINIQNQKYARLSAEDETETESGNASTAKNI"
     gene            2407..2604
                     /locus_tag="ISB30_001346"
     CDS             2407..2604
                     /locus_tag="ISB30_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAP1988996.1"
                     /translation="MNTNRILRKKEVLHLTGNSSATLYRLISKGFFPLSKRLTGDNGR
                     AVGWLESDINNWVNSRMQAGE"
     gene            2606..3073
                     /locus_tag="ISB30_001347"
     CDS             2606..3073
                     /locus_tag="ISB30_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988997.1"
                     /translation="MKEIKSKTSSAKKTLVPWFETMGKQVGLSQETISAVLKEQRLRI
                     RKLNYASAYTVSNILIQDCGGADDMWSNYAKLALILERCKYIPCGSRACAYCSTAQQL
                     HYQGVDVTGEYALYQLPELFLDFLETNDKNSSYHWDYNKLVERHEDAQARSFG"
     gene            3161..4015
                     /locus_tag="ISB30_001348"
     CDS             3161..4015
                     /locus_tag="ISB30_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988998.1"
                     /translation="MNDSYYFRTSDPDYIRHAPSMLEILSKAFPTIITQQESEYELQR
                     LNHLLNKLKGSNRCYNIIAQKLRQCRNGSPCNSLICPHCQRERILAQLAMLHVLPGNS
                     AEYVGVVLFFNKDTQTPPPWKNIGALRAQIGRYKQRISRVLNRLGYAGPATGTFSMMR
                     HMPDGPEERIFWVPQLCLFLPNDSTLIKGLKAHMSRSGGAFIDASTLNTPVIVLRYKD
                     PARLISCALNPVWHTADYTLTDKDALVKSRMELLKGRTLLKSLLTLDSLGTGVVSFSF
                     GQPPGKVH"
     gene            4592..5989
                     /locus_tag="ISB30_001349"
     CDS             4592..5989
                     /locus_tag="ISB30_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1988999.1"
                     /translation="MSTEKDYPVKIPKWVYDRITEITDYVVGDTQWAVTRRQTVRWFL
                     AHIWLETDDDGWTICTVRDIRSSYASLLGQCEISYQGECFMSTLTDFLPTLSDIEFRA
                     GKASKDPEKRRASAWRFNPLLPVCDESARGKLNLVDLDTGESVSFRALLQGNGKAPGH
                     AIDVEKRQTALKQTEKDFLAKVGRGRMSIQFVKELRSREPDHYYRAGIRSLNHLYNGR
                     IEGQYVTYDHHYRLTFGGRYYDQAFQNLPNEFKAKFRTGLLNYDIEACNLACLNHLFR
                     QYDVDYRVKSSIYKTMMEHTGLTRKQCKQMVHTTTYRIGRVTIGVNDGLGAKVYGWCG
                     NSKKKALKILRWWNRYISPLKSALESLLERVHGAHRKSCSSPRNYHRYANEVGLILDL
                     NSEVYQREKTHHHQYARNKALLAFMICGVEQAYIREVVSMNPGRVCMLDHDGVVATGA
                     LSLPDWRGFTMKVKD"
     gene            6167..6791
                     /locus_tag="ISB30_001350"
                     /pseudo
     CDS             6167..6791
                     /locus_tag="ISB30_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830285.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6986..9643
                     /locus_tag="ISB30_001351"
     CDS             6986..9643
                     /locus_tag="ISB30_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016155849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HAP1989000.1"
                     /translation="MDELLTLGCIALFFALVVVPILAIIALSRSTAVRSELATLRRRV
                     EVLEQRGVTEAPATVVTTVPQSVSQMVVPVETSSPAVNIVLSEPVADVTPESVDPWRP
                     QPKPQNEALPTPAVEQSSAFGGVMSSLVRWFMQGNPLAKLGILLLFLGFSFLLRYTVE
                     HSLFPLELRLVATSLFAIALLVVGWRLRHKQLVYALIIQGGATGTLYLTVFGAFWLWQ
                     MLPMTLAFALLVAICAASVGLAIMQKALSLAMLASIGGYLAPLLLSTGGGSHVALFSF
                     YLLLSVGILAISIWQHWRELNLLGLLFTFGIGGLWGLDGYRSEYYLSSQLFLIANTII
                     FGVLSVALSLRAQEKGKQIIDGVLLFASPLVGFGMQYAITRHMEYGPALSALGYGGFY
                     LALAWLALRRYPSLGRPLVLAALALGGAFTTLAIPLALSARWTAMAWALEGLGILWLG
                     VQQQQRRMSYSGTALLVLAVCSALWAQMNGMSALSLVLIFAVLSLSWLAAAWLWRNIQ
                     LQGSWVLLAGGLIFWIIALIGASQLVLKKDSLVLSGVLALMAISVWGWRIVSGRLAWW
                     ELDVSKWLLWPTMLVMLLSQISQHEIFAAGWQNLAWCLALPAAGALLWRDAETLPPRL
                     SRLAHLSLFWMILLALAAELFWFAQDLPWGMAAWGSGLMMAAGGLLIFLVHEAVHRQL
                     WPFRSWPALYASQAMIPVAAVLVVLLVLTNLQDGVVYRQTWLPLVNPLEEGAAFALLG
                     LIVFYRVSQRFFPVQISVCRPWPLIALLALSFWWLNGILLRALAWYGEVPWSAETLWH
                     SRLIQTCFALFWMLAALVVMLRATRRCSRREWLCGSVLLGIVIVKLMLVDSARGGGLA
                     RAVTFIGVAILVLIVGYFSPLPPKAGEEK"
     gene            9640..11035
                     /locus_tag="ISB30_001352"
                     /pseudo
     CDS             9640..11035
                     /locus_tag="ISB30_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133578.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
     gene            11518..11715
                     /locus_tag="ISB30_001353"
     CDS             11518..11715
                     /locus_tag="ISB30_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989001.1"
                     /translation="MSNPGPLAALVYGFILIVLLSVSLILYSIGRTIKNEIGKGIAYA
                     LTGILLILSLLVGAFILTLLT"
     gene            11782..12168
                     /locus_tag="ISB30_001354"
                     /pseudo
     CDS             11782..12168
                     /locus_tag="ISB30_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(12791..13645)
                     /locus_tag="ISB30_001355"
     CDS             complement(12791..13645)
                     /locus_tag="ISB30_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1989002.1"
                     /translation="MKYRFINDHREIWSIVTMCRVLKVARAGFYVWLHRPVSAGEKDN
                     QRLLELIRDAYALSGGVYGYRRVHGDLREIGEVCSRNRVARIMKRNRIQAVHGYKVPR
                     GVRGRPSLIAPNRVQREFTMVKPNQVWVTDITYIRTWQGWLYLAVVIDLFARNVVGWS
                     MKPTLSRELALDALLMAVWRRKPAENVIVHSDQGSQYGSDDWQRFCRANNLAPSMSRR
                     GNCWDNAVAESFFSSLKKERIRKRIYKTRDMARADIFDYIEVFYNRTRRHSHLGGVSP
                     EAFEKASP"
     gene            complement(13642..13926)
                     /locus_tag="ISB30_001356"
     CDS             complement(13642..13926)
                     /locus_tag="ISB30_001356"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1989003.1"
                     /translation="MGTPRFTPEFKEEAVRQVTERGYSVADVSERLGVSAHSLYKWVR
                     SVRPDNSGHQAQDLLDARTEILRLKAQLKRTEEERDILKKAARYFAREPD"
     gene            complement(14037..15212)
                     /locus_tag="ISB30_001357"
     CDS             complement(14037..15212)
                     /locus_tag="ISB30_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAP1989004.1"
                     /translation="MLTDTQCRTAKPKEKLYRLNDFNGLYLEVKPNGKKAWRYRFKLN
                     GKSSMFALGEYPTVKLAEAREKCEQARKQVADGVSPTQARQLDKIRKVNDASNTFELI
                     AKEWLQMKDWAGITKTRRLDMLERVVFPAIGKLPVREITPHHILKILQETAKRGAPTV
                     AAEARRTISSVFELAVATLRADSDPVWPVRKALPANKTQHKQALNPQQIGKLLSCFDN
                     SRGSYQVNYCMWLMWWTLARPAEATEAEWTEFNLDNALWTIPAARMKARREHVIPLPF
                     QAVKMLRTLQGLTGHRQHLFPGRDNPLGPMTSHSLRQLLKSLGWSGTYSPHATRTTGS
                     TRLNELGYRPDAIEAQLAHADTNNVRRTYNHATYLDERKVMMQEWADMLDKWVAGFK"
     gene            complement(15427..16518)
                     /gene="ychF"
                     /locus_tag="ISB30_001358"
     CDS             complement(15427..16518)
                     /gene="ychF"
                     /locus_tag="ISB30_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP1989005.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEATNFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(16635..17219)
                     /gene="pth"
                     /locus_tag="ISB30_001359"
     CDS             complement(16635..17219)
                     /gene="pth"
                     /locus_tag="ISB30_001359"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP1989006.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGSGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            17497..17775
                     /gene="ychH"
                     /locus_tag="ISB30_001360"
     CDS             17497..17775
                     /gene="ychH"
                     /locus_tag="ISB30_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAP1989007.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(17830..19509)
                     /gene="dauA"
                     /locus_tag="ISB30_001361"
     CDS             complement(17830..19509)
                     /gene="dauA"
                     /locus_tag="ISB30_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAP1989008.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(19634..20581)
                     /locus_tag="ISB30_001362"
     CDS             complement(19634..20581)
                     /locus_tag="ISB30_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAP1989009.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(20732..21583)
                     /gene="ispE"
                     /locus_tag="ISB30_001363"
     CDS             complement(20732..21583)
                     /gene="ispE"
                     /locus_tag="ISB30_001363"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP1989010.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFRFLDYGDIISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(21583..22206)
                     /gene="lolB"
                     /locus_tag="ISB30_001364"
     CDS             complement(21583..22206)
                     /gene="lolB"
                     /locus_tag="ISB30_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAP1989011.1"
                     /translation="MTLPDFRFIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            22420..23676
                     /gene="hemA"
                     /locus_tag="ISB30_001365"
     CDS             22420..23676
                     /gene="hemA"
                     /locus_tag="ISB30_001365"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAP1989012.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEIIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            23718..24800
                     /gene="prfA"
                     /locus_tag="ISB30_001366"
     CDS             23718..24800
                     /gene="prfA"
                     /locus_tag="ISB30_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP1989013.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            24800..25633
                     /gene="prmC"
                     /locus_tag="ISB30_001367"
     CDS             24800..25633
                     /gene="prmC"
                     /locus_tag="ISB30_001367"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP1989014.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            25630..26022
                     /locus_tag="ISB30_001368"
     CDS             25630..26022
                     /locus_tag="ISB30_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAP1989015.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            26026..26835
                     /locus_tag="ISB30_001369"
     CDS             26026..26835
                     /locus_tag="ISB30_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAP1989016.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            26871..27725
                     /gene="kdsA"
                     /locus_tag="ISB30_001370"
     CDS             26871..27725
                     /gene="kdsA"
                     /locus_tag="ISB30_001370"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAP1989017.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(27874..27981)
                     /locus_tag="ISB30_001371"
     CDS             complement(27874..27981)
                     /locus_tag="ISB30_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAP1989018.1"
                     /translation="MTLAQFAMIFWHNLAAPILAGIITAVIVSWWRNRK"
     gene            complement(28386..29486)
                     /gene="chaA"
                     /locus_tag="ISB30_001372"
     CDS             complement(28386..29486)
                     /gene="chaA"
                     /locus_tag="ISB30_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAP1989019.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            29756..29995
                     /gene="chaB"
                     /locus_tag="ISB30_001373"
     CDS             29756..29995
                     /gene="chaB"
                     /locus_tag="ISB30_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAP1989020.1"
                     /translation="MSYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKSSNR"
     gene            30144..30839
                     /locus_tag="ISB30_001374"
     CDS             30144..30839
                     /locus_tag="ISB30_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAP1989021.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(30883..31236)
                     /gene="ychN"
                     /locus_tag="ISB30_001375"
     CDS             complement(30883..31236)
                     /gene="ychN"
                     /locus_tag="ISB30_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAP1989022.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            31421..32815
                     /gene="ychO"
                     /locus_tag="ISB30_001376"
     CDS             31421..32815
                     /gene="ychO"
                     /locus_tag="ISB30_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAP1989023.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANHFENSNDGLP
                     DLGMAPEHHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVVESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(32816..33466)
                     /gene="narL"
                     /locus_tag="ISB30_001377"
     CDS             complement(32816..33466)
                     /gene="narL"
                     /locus_tag="ISB30_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAP1989024.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(33459..35255)
                     /gene="narX"
                     /locus_tag="ISB30_001378"
     CDS             complement(33459..35255)
                     /gene="narX"
                     /locus_tag="ISB30_001378"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAP1989025.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQSDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            35594..36985
                     /gene="narK"
                     /locus_tag="ISB30_001379"
     CDS             35594..36985
                     /gene="narK"
                     /locus_tag="ISB30_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAP1989026.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         8918 a         9133 c         9809 g         9315 t
ORIGIN      
        1 tcccttctgg tcaaaatgga cgaagaaatg ctctgcttct tcgacagcaa cgggcgaacc
       61 accatgcccc gtaaagcttt ctccctgcgt actggcgtaa cccgccgcca gctgtttcca
      121 gacgccattt acttcattga acgggagttc tgacatgacc atgtttaccc agcgtgaaac
      181 ccgtatcctt gaccaggcgc gtgacattat tagccggtac taccagcgtg gggttcaact
      241 ctgttcccct gatgatgttc gacggtgcgt gatggttgag ctggcaccgc ttgaacatga
      301 agagtttggc atcatcctac tcgataatca taatcagctg ctgcaccgcg aaatcctgtt
      361 caggggtacg ctaaactcgg tcagcgttca cccccgtgaa gttattaagc gtgtgctgaa
      421 acataacgcc gccgccgtca tccttgtcca taatcacccc agcggcgagc ctgaacccag
      481 ccggtgtgac atccagttga ccaaaaagct tcaggagctg ctggaaatac tggatgtgcg
      541 gttacttgat cactttatcg tggcaggaac tgaaacggtg tcgatggctg agcgaggact
      601 ggtgtgatgc tgaaagttac ttggtttaat gtacgttatt gcgggttacg cgcatgagca
      661 ggacagcgtt gcgtcaggtc gtgccggtgg tgcaaaccac cgtgttcgat gcgtatattg
      721 tcggtctggc cccctcggga cggcgaggta tcaccagcct gttaaaccgc agcgccagca
      781 tacttaaaca cggtgcggat gcagcagact atccgtggga acaacttaac tacgccgccg
      841 ttgccaaagt gcgcgcggca ctgctggatg acggttacgc cgtgtcatcg gtcaacatgg
      901 cgctgtcagc actccggggc gtagcgcaga cggcctttaa cctcaattac atggatgccg
      961 aaacgctggc acgtatccgg tcagttaagc gcgtgagcgg cgatgtccag cgcaagggaa
     1021 gggcgctggg caggcaggaa atccgggtac tgattcaggc tgcaaaacag catccgcaat
     1081 ctgtacggcg ctgtcgcgat gtcgccattg tgctgatgct ttgcggaacg ggcctgcggg
     1141 ccggggaact ggttaaactt gagcgccggg actatgacaa cggcatcctg acggtgaggc
     1201 aagggaaggg gcgtaaatac cgtgaaattc acgtggcgga tgcggtggac aaggctattc
     1261 gtgcgtggct gaaagttggc gcagacgagg ctgacagtgc gttgtttagg cgaattcagc
     1321 gcagtggtaa ggtcgcaagc caaccgctga ctacgaccgg gttaacgggt atcctggctg
     1381 agttacagca aacggcaggt atagcacgtt ttaccccgca tgatatgcgg cgcaccttca
     1441 tcacccgttt gctggagcag ggcgtggata tcaataccgt ccgccagttg gcaggccaca
     1501 gcgacatatc cacgacgacg cgctatgact gtcgtggtga tactatgaag atcagtgcca
     1561 gcaagcgcat cagatgtttt tagcagttga ggcgttgcca gactcggttt cagtttcatc
     1621 ttcagcactc aaccgcgcgt acttttgatt ctggatgtta atttgtgcat tgatattttc
     1681 aggctgaagc atccacgcaa catcctcacg cgcccattct ttaccgtcag agagtcgttt
     1741 taaccggggt ttccatattc catactcatt aagtttttct gctaccgaag catatttctc
     1801 acgatctgac tcccccgtat gaagcttgtc aaaacgacct tttacaagct ggtaaacagt
     1861 atagatttcc agtgccttag gggacctggc cgggactatc atggggtgtt cctccggcct
     1921 ataaatcccg tttggctctc cctcattgag caggtcatta aacatgtggt tcacgcaggc
     1981 actcagcaca tctgccgctt tctccgcagc gaatttatcc gggctaaaac ccgctcccgt
     2041 cagtttccgg gcaatcagtg ccagtttttg ccgggtatcc cgtcccagat aaagcttctt
     2101 ctccagaaat tcaaagacct cgttactgct tttccgttcc cggtggcatc tggcgtcagc
     2161 cttgttgctt tggttaccaa tcatagtcac ctccatgaca gtgttttgta caaaatatca
     2221 ccattgagcc tcagtgaaaa ttgcttcttt atatgaccgt tctggagaca gaaatagatg
     2281 atctcgaatg acatccacat taaagatgcc cttaacgtgc tcaacgaagg catcgttgag
     2341 tgaatccttg cgttttttgg tggttgtaaa taacccgctc ctgaaacagc tcaaaggagc
     2401 acaagcatga acaccaaccg tattttgcgt aaaaaagagg tacttcatct gacggggaac
     2461 tcgtcagcga cgttgtaccg actcatctcg aaaggattct ttcctttgtc aaagaggcta
     2521 acgggcgaca atggccgggc ggtaggctgg ctggagagtg atatcaataa ctgggttaac
     2581 agccgtatgc aggcgggaga atgaaatgaa agagataaaa tcgaaaacca gttctgcgaa
     2641 aaaaacgctg gttccttggt tcgaaactat ggggaaacag gttggtttat cacaagaaac
     2701 gatatcagca gtgttaaagg agcagcgttt acggatacgt aagcttaact acgcatctgc
     2761 atatacagtg tccaatattc tcatacagga ttgcggcggg gccgatgaca tgtggagcaa
     2821 ctacgctaaa ctggcgctta tccttgagcg ttgtaaatac attccctgcg ggagcagggc
     2881 ctgtgcatat tgttctaccg ctcagcaatt acattatcag ggggtggatg taaccgggga
     2941 atacgcttta tatcagttgc ctgagttatt tttagatttt ttagaaacaa acgacaaaaa
     3001 ttcctcttat cattgggatt acaataagtt agttgagcgg catgaagatg ctcaggcgag
     3061 gtcattcggg taatagccat ttctgtatgg tgaaaaactg acatattacc ctgccactgc
     3121 attttggtgg cagggtaatc aatacagcta ggaatattaa atgaacgatt cttattactt
     3181 cagaacatct gacccggatt acattcgtca cgcaccgtca atgcttgaga ttttaagtaa
     3241 agcattcccc actattatta cccagcagga aagtgagtat gaactgcagc gtcttaacca
     3301 tttactaaat aaactgaaag gcagcaatcg ctgctacaac atcattgctc aaaaactaag
     3361 gcagtgcaga aacggatctc catgcaacag tcttatatgc ccgcattgcc agcgggaacg
     3421 cattctggcg caactggcta tgttgcatgt cttgccgggg aattcagcag aatacgtggg
     3481 agtggtcctg tttttcaaca aggatacgca aactccgccg ccatggaaga acattggggc
     3541 gctcagggcg caaatcggtc gttataagca gaggataagc cgggtactta accgcctggg
     3601 gtacgccggt ccggccacag gcaccttcag tatgatgcga catatgcctg atggcccaga
     3661 agagcgtatt ttctgggttc cacagttgtg tttgttcctg cccaatgaca gtacgttgat
     3721 taaggggcta aaagcgcata tgtcgcgaag tggtggggca ttcattgatg catcgacgct
     3781 taacacacca gtgatcgtgc tcaggtataa agatcccgcg agactgattt catgcgccct
     3841 gaatccagtc tggcacacag cagattacac actgactgat aaagacgctc ttgttaagtc
     3901 aagaatggag ctactgaaag gccggacact gttaaaaagt ctgcttaccc tggactcact
     3961 cggaacaggt gtcgtttctt tttcgtttgg gcaaccccca gggaaagtgc actgatttac
     4021 cggtaatgca gcaactgcaa tgcacttttg ctgttgatta aattgcagtg gaacgacgaa
     4081 taacagcggc aaccactgca ttttcgttgt tgtaaaactg caaagcttgc cagtgatttt
     4141 gatggcgctg atgcacttta acattccaac tgtctcgatt ttaacgcaac cgtctgtttt
     4201 ctgcaggggc tcacggatgc ctctgcacac ccgatcctta ccatcaactg acgagttagc
     4261 gcatctgtga gggctttctg tgtttctgtc agtgaatatt ctacacacaa tggggggagc
     4321 ggtcagcgag caccattgcc attgagcgca acggagagct tggggacatg cagcactacg
     4381 gaggcttcat ctccttctca tgtcattaaa cgatacggcg aacgagcgca gcgagtgagg
     4441 gaaggcgtca gcctgaacag ggggaaagat ttgcgaccgg agtggagcaa atcatgggcg
     4501 gagcccatcc tcgcgactgg ggttttgact ttcgaattgc ccagcgttga gtttggttaa
     4561 tgtagtgaga cgaggatgct gagaggggac cgtgagtact gaaaaggatt atccagtgaa
     4621 gatccctaag tgggtttatg accgtattac tgaaataacg gattatgtcg tgggggatac
     4681 gcaatgggct gttacccggc ggcagacagt acggtggttc ctggctcaca tatggctgga
     4741 aacagacgat gatggctgga ctatatgcac tgtccgggat atccgtagca gctatgccag
     4801 tctcctggga cagtgtgaaa ttagttacca gggggagtgc tttatgtcca cgctaactga
     4861 tttcctgccg acactttcgg atattgaatt tcgtgcagga aaagcgagca aagacccgga
     4921 aaagcgtaga gccagtgcat ggcggttcaa ccccctgctg cctgtgtgtg atgagtctgc
     4981 caggggtaag ttaaatctgg ttgacttgga tactggtgag tctgttagtt ttagagctct
     5041 gcttcagggg aacgggaaag ctcctgggca tgcgatagat gtagagaaac gccagacggc
     5101 cctaaaacaa acggaaaagg acttcttggc caaagttggg cgggggcgca tgagtataca
     5161 gtttgtgaag gaattgcgta gccgtgagcc ggatcactat taccgcgcag gaatacgcag
     5221 ccttaaccat ctctacaatg gcaggattga gggacaatat gtcacttacg accatcatta
     5281 tcgtctgacg tttggagggc ggtattatga tcaagctttc cagaatctgc ccaatgagtt
     5341 caaagctaaa tttcggaccg ggttactcaa ctatgacatc gaagcgtgca atctggcctg
     5401 tctgaaccat ctgttcagac agtatgacgt cgattatcgt gtgaagtcat cgatatataa
     5461 aaccatgatg gaacataccg ggctgacccg taaacagtgc aaacagatgg tccataccac
     5521 cacctacaga attgggcgtg tgactattgg tgttaatgat ggccttgggg caaaagtgta
     5581 tggatggtgc ggtaacagca aaaaaaaggc gctaaagatt ttgcgttggt ggaaccgtta
     5641 tatcagtcca ttgaaatctg cgcttgagtc attgctggaa cgtgtgcatg gcgcacaccg
     5701 caagagttgt tcatcaccgc gtaactatca caggtacgct aatgaagtcg gattaatact
     5761 ggatcttaat agtgaggtgt atcagcgtga aaagactcac catcatcaat atgcgcggaa
     5821 taaggcactg ttggcgttta tgatctgtgg tgtggagcag gcgtatatcc gtgaggttgt
     5881 cagtatgaac cctggtcgtg tctgcatgct ggatcatgat ggggtagtgg caacgggcgc
     5941 gctttcacta ccggactggc gtggtttcac gatgaaagtg aaggactagc ttactttctt
     6001 gaatttaact atctgcgatc tctgataagg gcgtattacg ttgtcaatca taatgggata
     6061 tgcgcattgg aacgatgtgc atatccagta agtgatccat gtccgctttg agctgtgagt
     6121 tcaacgggtc gatgcaacgc tgtccctaat caagggggac gttgccatga aacgaagaac
     6181 tcgtattaac tacacgccag agcaaaaggt gattatttgg gacagatata agcaaggtga
     6241 ttccctgcat gatatccccc agaatgttcg acagatttca ctctactatc atgcctacaa
     6301 tccaccaggc cggtggctac cgtcctcccg tgcgaaagcg gcaccggtta gcgttttcgc
     6361 ttgatgaaaa agaggagata tccagaggac tggtagcaaa acgcatcatc agggacatcg
     6421 ctgctaaatt atcaatagct ccctcaacga ttagccgcga gatcaggagg cacggcggtg
     6481 caaaacaata ccgtgcagca aaagccgatc ctgctgcgtg ggaaaatgct ctgagaccaa
     6541 aaccttgcaa gctaattgaa agccccacat tgtgtaaaat cattgcagag aagatgcatc
     6601 aggactggtc gccggaacaa attggcggtt ggctaaaacg ctgttatccc gataatcagg
     6661 aaatgcatgt gtcacatgaa atgatttata aaacgctttt tataccaacc cggggagctt
     6721 taaaaaaaga gctgcagcaa tgcctcagaa gcggaagagt agtgcgtaga tccaaaacct
     6781 catcacttaa agtaaacaga gatacattct ggtcacgaat gcccggttgt tcgtgctttg
     6841 cgttttaatc gcattttaca gtttctgcct gcgtgcagag gttgatggta ttttctgaaa
     6901 aagcggctcg ctttgctaat gcatatggca caaatacaac agtttgtgtc attctgaccc
     6961 atctttatac cgcgacggtg gaaacatgga tgagctgcta acccttggct gcattgcttt
     7021 attcttcgca ctggtggtgg ttcccatcct cgcgattatc gcgctcagcc gcagcacggc
     7081 tgttcgttct gagctggcga ccctgcgtcg ccgggtggag gtgcttgagc agcgcggtgt
     7141 gactgaagca cccgccacag tggtaacaac tgtgccgcag agtgtttcgc agatggttgt
     7201 gcctgttgaa acgtcttcgc ctgcggttaa catcgtgctg tcggagcctg tggctgatgt
     7261 cacgcctgag tccgttgacc cctggcgtcc acaaccgaag ccgcaaaacg aggcgctacc
     7321 cacaccagcg gtcgaacaat cttccgcttt tggtggggtg atgtcgtccc tggtgcgctg
     7381 gtttatgcag ggcaacccgc tggcaaagct gggtattctg cttctcttcc ttggcttctc
     7441 cttcctgctg cgttatacgg ttgaacactc cctgttcccg cttgagctac gtctggtggc
     7501 gacttcgctg tttgcgattg ccctgctggt agtgggctgg cgattgcggc ataaacagct
     7561 ggtttatgcg ctgatcatac agggtggagc tacggggacg ctctatctca ccgtttttgg
     7621 cgcattctgg ctctggcaga tgctgccgat gacgctggcg tttgcattac tggtggcgat
     7681 ttgcgcggcg agcgtgggac tggcgattat gcaaaaagcg ctcagcctcg cgatgctggc
     7741 aagtatcggt ggctatctgg caccgctgtt gctttccacc ggcggcggta gtcatgtggc
     7801 gctcttctct ttctaccttc tgctttccgt cggtattctc gccatcagca tctggcaaca
     7861 ctggcgcgag cttaatctgc ttggcctgtt atttaccttt ggtatcggcg gtctttgggg
     7921 actcgacggc tatcgatcag agtattacct gagcagtcag cttttcctga tagccaatac
     7981 gatcattttt ggtgtgctga gcgtggcgct atcgctacga gcacaggaga aaggtaagca
     8041 gattattgat ggcgtgctgt tgtttgcatc gccgctggtc ggttttggca tgcagtacgc
     8101 cataacccgg catatggaat atggtccggc gcttagcgcg ctgggttacg gcggtttcta
     8161 tctggcgtta gcatggcttg cattgcgtcg ctatccctct ttggggaggc cgctggtgct
     8221 ggcggcgctg gcgctgggtg gggcatttac cacgctcgct atcccactgg cgctttcggc
     8281 ccgctggacg gcgatggcct gggcgctgga aggtctcggc atcctctggc tgggcgtgca
     8341 acagcagcag cggcgtatga gctatagcgg cactgcgctg ctggtgctgg cagtgtgcag
     8401 cgcgctgtgg gcgcagatga acgggatgag cgcgctttct ctggtgctaa tctttgccgt
     8461 actcagcctc agctggctgg ctgccgcctg gctgtggcgc aatattcagt tgcagggtag
     8521 ttgggttctg ttggctggcg gtttgatctt ctggataatc gcgctcattg gggcatcgca
     8581 actggtgctg aaaaaggact cgctggtgct gtctggcgtg ctggcgttaa tggcgatatc
     8641 ggtctggggc tggcggattg tctctggtcg tctggcctgg tgggagctgg atgtcagcaa
     8701 atggttgctg tggccgacga tgctggtgat gctgctgtct caaatttcac aacatgagat
     8761 ttttgctgcg ggctggcaga atctggcctg gtgtctggcg ttacccgctg cgggggcatt
     8821 gctctggcgt gatgctgaga cgttgccgcc gcgcctctcc agactggcgc acctttcact
     8881 cttctggatg attctgctcg cgctggcggc ggagctattc tggtttgcgc aagatctgcc
     8941 atggggcatg gcggcgtggg gctccggcct gatgatggcc gcggggggcc tgctcatctt
     9001 tctcgttcat gaggcggttc atcgtcagct atggccattc cgaagctggc ccgcactcta
     9061 cgcttctcag gcgatgatac cggtagctgc ggtattggtt gttctgctag tactgacgaa
     9121 tttgcaggat ggcgtagttt atcgccagac ctggctgccg ctggtgaatc cactggagga
     9181 gggggccgct tttgcgctac tgggactcat tgtattttat cgcgtttcgc agcgcttttt
     9241 cccggtgcag atttcggtat gccgtccctg gccgctcatt gcactgctgg cgcttagctt
     9301 ctggtggctg aatggcatcc tgctgcgcgc attggcctgg tacggcgaag tgccctggag
     9361 cgcggaaaca ctgtggcatt cgcggctgat tcagacctgt tttgccctgt tctggatgct
     9421 ggcggcatta gtggtgatgc tacgtgcgac ccggcgttgc tcacgtcgcg aatggctttg
     9481 tggatccgta ttgttaggga ttgttattgt gaaactgatg ttggtagaca gtgcgcgtgg
     9541 cggcggcctg gcacgcgcgg tcacgtttat cggcgtggca attctggtgc taatcgtcgg
     9601 gtatttttca ccgttaccgc ccaaagcggg agaagaaaaa tgaaatggat gaaagcggtg
     9661 ttatgcagcg tgctactgag tactgcgggc gcggcggtca gcagcgatga agtgaaggaa
     9721 gcgccaacgg actacgcaac aggcgtcgcg ctggaaacca cgggggcatt cgccctggta
     9781 tcgcatttcg ctgccgcagg tggtgtatca gagcactgca tggccggatt tgcgcgatgt
     9841 gcgggttttt aaccatctgg gggatatggt gccgtttgcg ctggtggcgc agaaaaccca
     9901 gaccgttacg ccagagacag tggctctgcg tcttttcccg cttgatatgt cgccggttcc
     9961 gccgcgtgat gagggtcaac gtagtgggga tgctgttgtt ttacgttcaa aaaacggtat
    10021 tgaaattcat ttgcaaagcg atgatgtcac tgcgctcggg cagagctatt tactgacgtt
    10081 gccggaagag aggaaagact ctctttcact gacgcaattg cgtctgaact gggggacgcc
    10141 gacgggcaac tggcagggga aagcctcggt ttatgtcagc cgtgatttac gctactggcg
    10201 acccgtgcag gaagatgcgc cgttaatgga tctgacgcgg gatagcgatc gtctgaaaat
    10261 ggacgccatc agcaccaacc tgacattgtc acttgaagga aatcgctacc tgctggtgat
    10321 cctcaactcg caaagcccgg cactgacgtt aaacagcgtg agcgctattg ctgacagtaa
    10381 tgagccggag tccgaacgta ttgtgattgg cgctcgggcg gataaggtgt cggatgatga
    10441 agccgtctgg cgatggacac agccgcagcc cctgacatcg ttgcggatcg atctggaaaa
    10501 tgaaggcgtt ttaccggtgg aactggtctg gcgcagcggc gaaaaagaac cctggcagtc
    10561 actgacaaaa acggtgttgt atcgcctgga cgggaagcgt tcagaagata ttcgtcttcc
    10621 tggccagtta gttgaggctg ttcgaatacg tacaattaat gcgcgattgc cggaggcatt
    10681 gccagcattg agcggtgcgc gcgacagtta tcaactggtt tttaacacgc aaggcaaagg
    10741 tccttacatg ctggcgtggg gaaaccgggc ggcgaaaaag gccgatgttg gactcgatat
    10801 gttgatccct gcgtcgctgc gtaaaacgca ggagatagat aatctgccgt gggctatacc
    10861 gcaggagagc gtaactctcg gcggtgaatt gcgactaacg gcgacgtcgg cagccgaaca
    10921 acaaagtcag tggaaaacgc tgctggtgtg gggggcgtta atacttggcg ttgccgttct
    10981 ggcgtttatg gcatggcgaa tctggcgaga agttaaaaag gacggtgcgg cgtaagccat
    11041 aaaaaagcac agttgcatag ttcacggtgt tgataaactg ttacaacaga ggcgtttaag
    11101 ctgaggcggg gaaataatct atttgtgaat aatatgttac ttcactgggg cgtgagtcat
    11161 gggaatgatt ttgatacctg cgttttttat tttagccatc ctgattatcg ctatcttcat
    11221 ttcagttaag aatggccgtc acgtatccgt tgtatgcctt gttgccgtgg gtgagatttt
    11281 cgtattccca ttctatttta cggcccttaa gcgtttccgg agcatcaaaa cttggtaaag
    11341 atttaccatg ttgacccatt atgcggcccc tctggagacc actttcaggt attttctcta
    11401 ctgatatgta cacatggaga gatgtgattg caaattccca tcagcattac taccaaaaga
    11461 atcagacagg aagcagcata gtattaataa gaatattttc aaacataaag gatgattatg
    11521 agtaacccag gcccacttgc agcgttagtg tatggtttta ttttgatagt tttgctatcg
    11581 gtatctttaa tcctttattc aattggacgt accataaaaa acgagattgg aaaaggtata
    11641 gcatacgcgc ttactggtat tttattaatt ctgtcattat tagttggcgc tttcattttg
    11701 acgcttctta cataaattcc tcgaaaaata cgctgacagc atcagctaat ggacccgact
    11761 ctccgttgaa cgcatgctcg cctgcggcac ctgtgccatg ggcgtccgcc gctacgggca
    11821 ggcatcaagg ttcgcttcac aacagcggac ctgctgctac agctatccac ttcatagcgc
    11881 cagggccgtt acaaaacgac actcaatcgc ggtgtcatgg ccccgaagct acttatcatc
    11941 gatgaaatag gttatctgcc gttcagtctg gaagaagcca agctgttctt ccaggtcatc
    12001 gctaaacgtt acgagaggag cgcgatgatc ctggactcca acctgccgtt cgggcagtgg
    12061 gatcagacgt tcgccaggga tgcagccctg acatcggcga tgctggaccg tatcttacat
    12121 cactcacacg tagtccagat aaaaggagag agctatcgac tgaagtagaa acgaaaggcc
    12181 ggggttatac ctgaagctaa tcctgagtaa acaaggtgga tcaatattaa accgttggtg
    12241 gtgacggtta gtggatcact ttattacccg ttgttgacag taaacgcagg aagtcagatg
    12301 tgatgcgccg aaagctgctt tcgcacatgt tacagggact ggaaagccgg gctgtttacg
    12361 attacgttgc cagtcataag ggcgtttgcg cactggagca cgatgggttt ttttcatata
    12421 aagaggtcac tgactggtcc catccatatc tgttgattga gaaaaagcat taaggggagc
    12481 cactcagtca attacagctt atctcggttg ataaacaaga cgggacattt agcactttca
    12541 tgatatgcat agccggcagg tgatcttagc ctgctttcag cgatgaacca cacaatattc
    12601 cccttttgtt actttgatat gattgctcaa aatgggtggt aacactgaga tcgttgaaat
    12661 gactgcatgt tatattgtta cttttgttac cgttgaaaac atcacgttgg agagctaaat
    12721 ggattggcca aaggagtaca gcaagacgac tcaagtggag tgaccacatt ccggtagaca
    12781 gtttctgtcc tcacggtgag gccttttcga aggcctccgg gctgacgcca cccagatgac
    12841 tgtggcgccg ggtccggttg tagaacactt caatgtaatc gaagatatcc gcccgggcca
    12901 tgtcccgggt tttatatatc cgctttctga tgcgctcttt tttcagtgaa ctgaagaacg
    12961 attctgccac cgcattatcc cagcagttgc cacgacggct catactcggc gccagattgt
    13021 tggccctgca gaaccgctgc cagtcatcac tgccatactg actgccctga tcgctgtgca
    13081 ctatgacgtt ttctgcaggc ttacgccgcc agactgccat cagcagcgca tccagtgcca
    13141 gctcgcgcga cagcgttggt ttcatcgacc agcccaccac attacgggca aaaagatcga
    13201 taaccaccgc cagatacagc caaccctgcc aggtgcggat gtaggtaata tcagtcaccc
    13261 acacctggtt gggttttacc atcgtaaatt ccctctgtac gcgattagga gctatcagtg
    13321 acggccttcc cctgacgcca cgcgggacct tatagccatg tacggcctgt atcctgttcc
    13381 gcttcatgat tctggcaaca cggtttctgc tgcatacttc accgatttca cgaagatcgc
    13441 cgtgaaccct gcgataaccg tagactccgc cgctcagtgc ataagcatcg cggatgagct
    13501 ccagcaaccg ctgattatcc ttttctccgg cagaaacagg gcgatgaagc cagacgtaaa
    13561 atcccgcacg ggcgaccttc agaacccgac acattgttac gatcgaccag atttcacggt
    13621 gatcgttgat gaagcggtac ttcagtcggg ctcccttgca aagtaccgcg ctgccttttt
    13681 cagaatatcc cgttcttcct cagtacgttt aagctgcgct ttaagcctga gaatctccgt
    13741 ccgcgcatcc agtaaatcct gcgcctgatg cccgctgtta tccggtctga ctgaccggac
    13801 ccacttataa aggctgtgag ccgatacgcc gaggcgttca gaaacatcgg caacggaata
    13861 gccccgttca gtgacctgac ggacagcttc ttccttaaac tctggggtaa atcgtggtgt
    13921 gcccatagac tcctcctatg ctcaaactat agggcagatt tgtctacggg cttgggggca
    13981 ctccagaacc ttgaggaata agttctatcc tcgtggttca ctttgtgcag caagttctac
    14041 ttgaatcctg ctacccattt gtccaacata tcagcccatt cctgcatcat cactttacgc
    14101 tcatcaagat aagttgcatg gttgtatgtg cgacgaacat tgttggtatc agcgtgcgca
    14161 agttgggcct caatagcatc aggtcgatag cccaactcgt ttaaacgggt acttcctgtg
    14221 gtccgggttg catgtgggct atatgtacca ctccagccga ggcttttaag tagctggcgc
    14281 agtgagtgcg atgtcattgg acctagcgga ttgtctctgc ccgggaaaag gtgctgccga
    14341 tgcccggtta atccctgtag tgttctgagc attttgacag cttggaaggg taggggaata
    14401 acgtgttctc tgcgggcttt cattcgcgct gccggaatag tccatagggc gttatcaaga
    14461 ttgaattctg tccattctgc ttcggtggct tctgcaggac gcgccaatgt ccaccacata
    14521 agccacatgc aatagttaac ctgatacgag ccacgactat tgtcaaaaca gcttagtaac
    14581 tttccgattt gttggggatt tagtgcctgt ttgtgctgtg tcttattagc cggaagggcc
    14641 ttgcgcacag gccatacggg atcgctatca gctctaagcg tcgcgactgc caactcaaaa
    14701 actgatgaaa tggtgcgacg ggcttcagcg gcaactgttg gtgctcctcg tttagcggtt
    14761 tcctgaagaa ttttaagtat atggtgtgga gtgatctctc tgacagggag cttaccgatg
    14821 gcgggaaaaa ccacacgttc aagcatatcc agtcgccgcg ttttggtaat tccggcccag
    14881 tctttcatct gcaaccattc tttagcgatt agctcaaagg tgttggatgc gtcgttgacc
    14941 ttgcggatct tatctaactg acgagcctgt gttgggctaa ctccatctgc gacttgctta
    15001 cgcgcttgct cgcatttctc gcgagcttcg gcaagtttta ccgtcgggta ctcacccagc
    15061 gcaaacatgc tggatttacc gttgagttta aaccgatagc gccatgcctt tttaccattg
    15121 ggtttcactt cgaggtagag accattgaaa tcattaagtc gatagagttt ttctttcggc
    15181 ttagcagtgc ggcattgcgt atcagtgagc ataacggggc cttgtttatt tattataccg
    15241 ttattgtact cacttaaaat gaagatggaa gaatgttgta ctcatccatg tactcatttt
    15301 tgacagcgtt gttatgagat gaattgagac attgcgaaat gaaaaatcca cgcaattgcg
    15361 tggattttat agagttttac ggttcttatg agattcagtg aaacctcatg agacagcaga
    15421 tttaatttag acgttgaaca ggaagttcat cacatcgcca tctttaacga tgtaatcttt
    15481 gccttctgca cgcattttgc ctgcttcttt cgcgccttgt tcacctttgt aagtgatgaa
    15541 atcttcaaac gagatggttt gtgcacggat aaagcctttt tcaaaatcag tatggatttt
    15601 gcccgctgcc tgcggcgcgg ttgcaccaac cggaatggtc catgcacgca cttctttcac
    15661 cccagcggtg aagtaagttt gcaggttcag cagcttataa ccggcacgga tcacacggtt
    15721 caggcccggc tcttccagcc caagctcctg cataaactcg tcacgctctt cgtcgtccag
    15781 ttcggcaatg tctgcttcaa cagcagcaca aaccggaact acaacagaac cttctttcgc
    15841 tgcgatttca cgcacctggt caagatacgg gttgttttca aaaccgtctt cgttgacgtt
    15901 ggcgatgtac attgttggtt ttagcgtcag gaagctcagg taacgaacag ccgctttctc
    15961 ttcagcgctt aaatccagcg cgcgcagcat acctgcgttt tccagctggg gcaggcattt
    16021 ttccagtacc gccagctcgg ctttcgcgtc tttatcgcca cctttggctt tcttctgtac
    16081 gcgatgaatc gcacgttcac aggtgtcgag atccgccagc gccagttcgg tgttgataac
    16141 ttcaatatca tccgccgggt taactttgcc ggaaacgtga atgatgttgt cattttcaaa
    16201 gcagcgaaca acgtgaccga tcgcttcggt ttcacggatg ttggtcagga actggttacc
    16261 cagaccttcg cctttcgatg cgccttttac cagaccggcg atatcgacaa attccatggt
    16321 cgtgggaagt gtacgctgcg gttttacgat ttcagccagt tgatccaggc gagggtcagg
    16381 cattggtacg acgccagtgt tcggctcaat ggtgcagaat ggaaagttgg tcgcttcaat
    16441 accggctttg gtcagcgcgt tgaacagggt agatttcccg acgttgggca aaccgacgat
    16501 accgcatttg aatcccatga tttaaatcac cttaatatct taataatcaa cctgttattg
    16561 ctaacagatt gcagaaatgg aaataacttt gcctattata cacggcactc ggcaaaaatg
    16621 ccgcagacaa cgacttattg cgctttaaag gcgtgcaatc ggttcgttgc tttggtcaag
    16681 ccatctgtaa accacatttc agtacaacgc gccgcttcgt cgatggcttc atcaattaac
    16741 ttctgttcac taacaggcgg tttgcctaac acaaaaccga caactttatt tttatcgccc
    16801 ggatgaccga ttccgatgcg taaacggtga aagttagggt tattacccaa tttactgatg
    16861 atgtctttca gtccattgtg accaccatga ccgctaccca acttaaattt ggcgacgcca
    16921 ggaggcagat ccagttcgtc gtgggccacc agaatttcgt ccggattaat gcggaaaaaa
    16981 ctggccatcg ccgcaacggc tttgccgctg agattcataa atgtagtcgg gactaacagg
    17041 cggacatctt cgcctccaag ggtgactcgc gaagtataac caaagaattt agcctcttcg
    17101 cgcagcggag cgcgcaatcg ctctgccagt aagtcaacga accaggcacc agcattatgt
    17161 cgcgttgcgg cgtattcagc accggggttc gccaggccga caatcaattt aatcgtcacg
    17221 tttttttgtc ctgagtgtgt acataactgg cgcgtagttt actggttgcg gccccgcttg
    17281 acaaaaaact gcgtatcaaa tgcagataat gcaattattg cctaagtgga ctattagaaa
    17341 gtcaaggtgt tcaggcgttt atttgtaaag ttttgttgaa ataagggttg taattgtgat
    17401 cacacccgca cataacccac agggtgttgt ctatacttta cacataagga agaggggtat
    17461 tccctgttac aacccagaaa gttccggagg tgacatatga aacgcaaaaa cgcttcgtta
    17521 ctcggtaacg tgctcatggg gttgggtctg gtggtaatgg tggtcggcgt ggggtattca
    17581 atcctcaacc agttaccaca gtttaatatg ccccagtatt tcgcacatgg tgctgtgcta
    17641 agtattttcg tcggtgcgat tctctggctg gcgggtgccc gtgttggcgg gcatgaacag
    17701 gtgtgcgacc gttactggtg ggttcgccac tatgacaaac gttgccgccg tagcgataat
    17761 cgccgtcata gctaacaaat aatgcagttt gctgaccagt cagtttgcgc tgactggtca
    17821 atctcgtact tataaatccg ccatcgccgc gcgacgattc gggaagaatg cgaggcgtcc
    17881 cgggatcggt tgaatgcctg cgcgagccat agtgcgcagt ggctggaatt ccaggttgca
    17941 cacgcgcagt tcacatcctt cgggcagacg cttcacaaaa cgctggaacg catcaagacc
    18001 accagcatca agtaccggaa cggcatccca cttcagaatc acaatccgtt tgccttcaag
    18061 acgtgactcc aggtccgtga ataagccttc agcggcagca aaaaacagcg ggccaataac
    18121 gcgcagaacc aggacatcgt ctggaacgtc tacgactacc ggtgccaggc gagtcatacg
    18181 tgcgatacga cgcataaaca gcagcgatgc cagcacgatc cccacgctga tggcaataac
    18241 catatcgaac agcacggtca gcgacatgca cagcagcatg acgatgatgt catctttcgg
    18301 tgcatgacgc agcaagtcga ccactttgtg cgcttcactc atgttccacg ccaccatcaa
    18361 cagcagggct gccatggcgg aaagtggcag ccaggagagc agcggtgcca gtaccagcag
    18421 ggcaagaata accagaatag agtggatcac cgccgagata ggggaagttg ccccggcacg
    18481 gacgttagcg gcagaacgcg cgatggcagc tgtagcggta atgccaccaa agaacggagc
    18541 gataatattc cccagtccct gtccaaccag ttcgctgttc gccttgtgtt tcgtcccggt
    18601 cataccatcc agcaccacgg cgcagagcag agattcgatt gcgccgagca ttgccattga
    18661 gaatgccgca ggcagcagtg tgcgaataga atcccaggtt agcgtgaatt ctgaattagg
    18721 cagatcccac ggcagcacca gttgcggcag cagttgcgga ataccgttac cctgagaacc
    18781 atcggccagg acgtagtgga attgcgaacc gatggtagca acatgtccgc cgagcaggtt
    18841 aacaatcccc atcaccgcgc aaccagccag caaggccgga aggtggccgg gtaaacgtat
    18901 gcccagacgc ggccagaaaa ccagaatgcc aagcgtcaca atgccaatgg ctgcatcacc
    18961 cacattaatg gtcggcagcg ccataaataa tgcgccgact ttttgtagat aatgttccgg
    19021 gacatgggcc atttgcagac cgagaaaatc tttaatctgc atggtaccga tggtgatccc
    19081 gatacccgag gtgaaaccta aggtgacgga aaccggaata tactcaatca ggcgaccaaa
    19141 gcgtgccagc cccatcagaa tcaaaaagat ccccgacagc aaggtcgcaa ccagcagtcc
    19201 tgccagtcca aactgttgcg aaacgggata gagaattacc acaaatgccg cagtcggacc
    19261 ggaaacgcta aagcgtgacc ctcccgtcag agcaatgaca atccccgcaa cagctgcggt
    19321 atataaaccg tactggggtg ccacaccact accaatagcc aacgccatcg ccagcgggat
    19381 agcaataatc ccgacggtta tcccggcaat caggtcacgg gtaaaccgtg cggcagtata
    19441 tttttctttc cagcaagcgt cgatcagagc gcggaaaggc atcacatgtg aggaaaatat
    19501 tttgttcaca ataatgtttc atccgtgagc gcatcatctg tcaactaaat ggcaggtgaa
    19561 ggaggcatag gtcatacaaa tggatattac agacaaaaaa acccgccgca gcgggtcttt
    19621 gagccgggtt cgattagtgt tcgaacatgg cagatatcga ttcttcgttg ctgatacgac
    19681 gaatcgcttc ggccagcata cctgacaggg tcagagtacg cacgttcggc agtgatttga
    19741 tttcatcgct cagcggaatg gtatcgcaga caacgacttc atcaattaca gagttacgca
    19801 ggttgttcgc cgcgttgcca gagaagatcg ggtgagtcgc gtacgcaaat acacgtttag
    19861 caccacgttc tttcagagct tcagcagctt tacacagcgt accgccagtg tcgatcatat
    19921 catcgaccag tacgcagtca cgacctgcaa cgtcaccgat gatatgcatc acctgggaaa
    19981 cgttcgcacg cggacgacgt ttgtcgatga ttgccatatc ggtatcgttc agcagcttag
    20041 cgatagcgcg ggcacgcaca acgccgccga tgtccggaga aaccacaatt gggttatcca
    20101 gattcagctg cagcatgtct tccagcagga tcgggctacc aaatacgtta tcaaccggaa
    20161 cgtcgaagaa accctgaatc tgttcagcgt gcagatccac tgtcagcaca cggtcaacac
    20221 cgacgctgga gaggaagtct gcaaccactt tcgcagtgat tggtacacga gcggaacgga
    20281 cgcgacggtc ctggcgcgca tagccaaagt aggggataac agcggtgata cgacctgcgg
    20341 aagcacgacg cagggcatca accataacga ctaattccat caggttgtcg ttagtagggg
    20401 cacaagtgga ctggatgatg aaaatatcac caccgcgtac attttcatta atttgtacac
    20461 tgacttcgcc gtcgctaaag cgacctacag cggcgtcgcc gagtgaagtg tacaggcggt
    20521 tggcaatacg ttgtgctagt tccggggtgg cgttaccagc aaaaagcttc atatcaggca
    20581 cgagaagaac ctcaggcatg cgtccattgg tggaaagaat ctgccgaaaa ctgtgcgggc
    20641 caggcatgat tctttccagg cggtgtatta aagagcgcga tgcaacgtct ggaacaaggt
    20701 gacgttgtca ccgaaactca gcttgcccgg cttaaagcat ggctctgtgc aatggggaaa
    20761 gattaacgcc tttcgccaca aagccattga gccattccgg ggcttgctct agcacctggc
    20821 gggcttcaga ctctgtatca aattcagcaa agacacaggc ccctgtccca gtcaggcgcg
    20881 acggggcgta ttctaacagc caggaaagca ccgcatcaac ctcgcgaaaa cgttttcttg
    20941 cgataacctc gcaatcattg ctgaattcac attttagcaa cgtttctatt gacctttttg
    21001 gcgtattgcg cgggagttca ggatctttaa aaatcaccgg tgtcggaata cttacaccag
    21061 ggtgcgccac cagataccac ttctctggcg gatccaccgg cgttagtatt tcaccaacgc
    21121 cttcggcaaa cgcggcatga ccacgaacaa agacaggaac atctgcgccc agcgtcagcc
    21181 ccatttccgc cagctcatcc atgcttagtc cgcattgcca gagatgattt aacgccacca
    21241 gaaccgtcgc ggcattggat gaaccaccgc cgagaccgcc gcccatcggc aaacgcttgt
    21301 caatgctgat attcgcaccg cttcccgtcg aaagacgccc gctgtctgcc gcagttttca
    21361 tcaacaaccg cgctgcgcga acgatcaggt tatcttcatg ttccacgcct tcaacgggcg
    21421 ttaacagacg aatatcccca tcgtcacgaa gctcaatgct gatgatgtcg ccgtaatcaa
    21481 gaaaccgaaa cagcgtttgc agcgtgtggt aaccatccgc acgctgaccg gtaatgtata
    21541 aaaacagatt aagttttgcc ggagagggcc actgtgtccg cattatttca ctatccagtt
    21601 atccattttt aacttgatgc gttgaccacc gtcggtgagt tccatattgg ctggcatcgc
    21661 aggttgagtt tttgtgtcat aaccaccgta aacaactttc cagtttttgc cattctggct
    21721 gtaggtgatt tcgctcaggc ggtactggtc gtccagtttg taatcggtcg catcacccgg
    21781 taaacctaaa atccactggc gcaagctgtt gagcggaatc ggcattccgg ttagtttacc
    21841 aatcatctct tcggcgtcat cggcggtgta acgctgacct ttattgtcga ctaattgtac
    21901 gttacccggc tgagcattca gctccagttc cgtgctgccc aatgggttag ttaacagcag
    21961 acgatagcga tcctggccgg tttgttgcca gaagaagcga gcgtacactt tttgttggtc
    22021 ggaaatataa gcgaatgcac cgcgagtctg atactgatta agattacgca cgtcttgctg
    22081 gtgctgacgc cactgtggcg aatccgggct tttgcctggg cctttgggtg tggtaacaga
    22141 acaggcagtg agcacgaggg cagctagcgg tagcaggcgg ataaagcgaa aatcgggcag
    22201 ggtcataata atgacaagtc cttgagatac gttgcagtta taacccttaa tgctagcgtt
    22261 accgttagcc atcgtctatg ttcacgttgt cttaattgcc agaatctaac ggctttcggc
    22321 aattactcca aaagggggcg ctctctttta ttgatcttac gcatcctgta tgatgcaagc
    22381 agactaaccc tatcaacgtt ggtattattt cccgcagaca tgaccctttt agcactcggt
    22441 atcaaccata aaacggcacc tgtatcgctg cgagaacgtg tatcgttttc gccggataag
    22501 ctcgatcagg cgcttgacag tctgctcgcg cagccgatgg tgcagggcgg cgtggtgctg
    22561 tcgacgtgta accgcacaga actttatctc agcgttgaag aacgggatga cctgcaagag
    22621 gcgctcattc gctggttgtg tgattaccat aatctcaacg aagacgatct gcgtaatagc
    22681 ctctactggc atcaggataa cgatgcggtc agtcatttaa tgcgcgttgc cagtgggctt
    22741 gattctctcg tccttggcga gccgcagatc ctcggtcagg ttaaaaaagc gtttgccgat
    22801 tcgcaaaaag gtcatatgaa ggccagcgaa ctagaacgca tgtttcagaa atctttctct
    22861 gtggcgaaac gcgttcgcac tgaaacagat atcggtgcca gtgctgtgtc tgtcgctttt
    22921 gcggcttgta cgctggcgcg gcagatcttt gaatcgctct ctacggtgac agtactgctg
    22981 gtgggtgcgg gcgaaaccat cgagctggtg gcgcgtcatc tgcgcgaaca caaagtacag
    23041 aagatgatta tcgccaaccg cactcgcgaa cgtgcccaaa ttctggcaga tgaagtcggc
    23101 gcggaaatta ttgccctgag tgatatcgac gaacgtctgc gcgaagccga tatcatcatc
    23161 agttccaccg ccagcccgtt accgattatc gggaaaggca tggtggagcg cgcattaaaa
    23221 agccgtcgca accaaccaat gctgttggtg gatattgctg ttccgcgcga tgttgagccg
    23281 gaagttggca aactggcgaa tgcttatctt tatagcgtgg acgatctgca aagcatcatt
    23341 tcgcacaacc tggcgcagcg taaagccgca gcggttgagg cggaaactat tgtcgctcag
    23401 gaaaccagcg aatttatggc gtggctgcgt gcacaaagcg ccagcgaaac tattcgcgag
    23461 tatcgcagcc aggcagagca cgttcgcgat gagttaaccg ccaaagcgtt agcggccctt
    23521 gagcagggcg gcgacgcaca agccattatg caggatctgg catggaaact gactaaccgc
    23581 ttgatccatg cgccaacaaa atcacttcaa caggccgccc gtgacgggga taacgaacgc
    23641 ctgaatattc tgcgcgacag cctcgggctg gagtagcagt acatcatttt ctttttttac
    23701 agggtgcatt tacgcctatg aagccttcta tcgttgccaa actggaagcc ctgcatgaac
    23761 gccatgaaga agttcaggcg ttgctgggtg acgcgcaaac tatcgccgac caggaacgtt
    23821 ttcgcgcatt atcacgcgaa tatgcgcagt taagtgatgt ttcgcgctgt tttaccgact
    23881 ggcaacaggt tcaggaagat atcgaaaccg cacagatgat gcttgacgat cctgaaatgc
    23941 gtgagatggc gcaggatgaa ctgcgcgaag ctaaagaaaa aagcgagcaa ctggaacagc
    24001 aattacaggt tctgttacta ccaaaagatc ctgatgacga acgtaacgcc ttcctcgaag
    24061 tccgtgccgg aaccggcggc gacgaagcgg cgctgttcgc tggcgatctg ttccgtatgt
    24121 acagccgtta cgccgaagcc cgccgctggc gtgtagaaat catgagcgcc agcgagggtg
    24181 aacatggtgg ttataaagag atcatcgcca aaattagcgg tgatggtgta tatggtcgtt
    24241 tgaaattcga atctggcggt catcgcgtgc agcgtgttcc tgctacggaa tcgcagggtc
    24301 gtattcatac ttctgcttgt accgttgcgg taatgccaga actgcctgat gcagaactgc
    24361 cggacatcaa cccagcagat ttgcgcatag atactttccg ctcgtcaggg gcgggtggtc
    24421 agcacgttaa caccaccgat tcggcaattc gtattactca cttgccgacc gggattgttg
    24481 ttgaatgtca ggacgaacgt tcacaacata aaaacaaagc taaagcactt tctgtactcg
    24541 gtgctcgcat ccacgctgct gaaatggcaa aacggcaaca ggccgaagcg tctacccgtc
    24601 gtaacctgct ggggagtggc gatcgcagcg accgtaaccg tacttacaac ttcccgcagg
    24661 ggcgcgttac cgatcaccgc atcaacctga cgctctaccg cctggatgaa gtgatggaag
    24721 gtaagctgga tatgctgatt gaaccgatta tccaggaaca tcaggccgac caactggcgg
    24781 cgttgtccga gcaggaataa tggaatatca acactggtta cgtgaagcaa taagccaact
    24841 tcaggcgagc gaaagcccgc ggcgtgatgc tgaaatcctg ctggagcatg ttaccggcaa
    24901 agggcgtact tttattctcg cctttggtga aacgcagctg actgacgaac aatgtcagca
    24961 acttgatgcg ctactgacgc gtcgtcgcga tggtgaaccc attgctcatt taaccggggt
    25021 gcgagaattc tggtcgttgc cgttatttgt ttcgccagcg accttaattc cgcgcccgga
    25081 tacggagtgt ctggtggagc aggcactggc gcggttgcct gaacaacctt gccgtattct
    25141 cgatctcgga acgggtaccg gggcgattgc gcttgcgctt gccagcgagc gcccggactg
    25201 cgaaattatc gctgtagatc gtatgcccga tgctgtcgcc ctggcacaac gtaatgccca
    25261 gcatctggct atcaaaaata tccacattct gcaaagcgac tggtttagcg cgctagccgg
    25321 gcagcagttt gcgatgattg tcagcaatcc gccgtatatt gacgagcagg acccacatct
    25381 tcaacaaggc gatgtccgct ttgagccgct cactgcgctg gttgcggcag acagtggaat
    25441 ggcagacatc gtgcatatca tcgaacagtc gcgtaacgcg ctggtatccg gcggctttct
    25501 gcttctggag catggctggc agcagggtga agcggtgcga caggcattta tcctcgcggg
    25561 gtatcatgac gtcgaaacct gccgtgacta tggggataac gagcgcgtaa cgctcggccg
    25621 ctattatcaa tgacaagttt ttctacgttg cttagcgttc atctcattag tatcgcgctt
    25681 tctgttgggc tattgacctt acgtttctgg ctacgttatc agaagcatcc acaggcattt
    25741 gctcgctgga cgcgcattgt gccgccggtt gtcgatacgc tgttattgtt aagcggcatt
    25801 gcgttgatgg ctaaagcgca catccagcca ttttccgggc aggcacagtg gctgactgaa
    25861 aagctgtttg gagttatcat ttatatcgtt ttgggtttta ttgcactcga ttatcgtcgg
    25921 atgcacagtc agcaggcgcg cattattgcc ttcccgctgg cgttggtggt gctgtacatc
    25981 atcattaaac tcgccaccac aaaagtaccg ttactggggt aagtcatgag atcgttagct
    26041 gatttcgaat ttaataaagc gccattgtgc gaaggcatga tcctggcttg cgaagcaatc
    26101 cgccgcgatt ttccctcgca agatgtttac gacgaactgg agcgtctcgt tagtctggcg
    26161 aaggaagaaa ttagccagct tctgccttta gaagagcagt tggaaaaact gatcgcgctg
    26221 ttttacggcg actggggatt taaagcctca cgcggcgttt atcgtctttc cgatgcatta
    26281 tggctggacc aggtgttaaa gaatcgacag ggcagtgcgg tatcattagg tgcggtttta
    26341 ttatgggtcg cgaatcgtct cgatttgccg ctgctgccgg tgattttccc tacgcagctg
    26401 atattgcgca ttgaatgtcc ggatggcgaa atttggctga ttaatccttt taacggtgaa
    26461 tcgttaagcg aacatatgct ggatgtgtgg ttaaagggaa atatcagccc gtcggcggaa
    26521 ctgttttatg aagaccttga tgaagctgat aacattgagg taatccgcaa attgctggat
    26581 acactcaaag cttcgttgat ggaagaaaat cagatggagc tggcgttacg caccagcgaa
    26641 gctttattac aattcaaccc ggaagatccc tatgaaattc gcgatcgcgg gttgatttat
    26701 gcgcaactgg attgcgaaca cgttgcgttg aacgatttaa gttatttcgt tgaacagtgt
    26761 ccggaagacc cgatcagcga aatgatccgt gcgcaaataa ataacatcgc gcataaacat
    26821 attgtgctgc attaattaat cgacatttta ctcaagatta aggcgatcct atgaaacaaa
    26881 aagtggttag cattggtgac atcaacgtag caaatgacct gccgttcgta ctgtttggcg
    26941 gtatgaacgt gctggaatct cgcgatctgg cgatgcgcat ttgcgagcac tacgtaaccg
    27001 tgacccagaa actgggtatc ccttacgtgt tcaaagcctc ttttgacaaa gccaaccgct
    27061 cctccatcca ctcttatcgt ggaccgggcc tggaagaagg gatgaaaatc ttccaggagt
    27121 tgaagcagac ttttggcgtg aaaattatca ccgacgttca cgaaccaagt caggcacagc
    27181 ccgttgctga tgtcgtggat gtgattcagt tgccggcgtt tcttgctcgc cagactgacc
    27241 tggttgaagc catggcgaaa accggtgcgg taattaacgt caagaaacca cagtttgtca
    27301 gcccgggaca gatgggtaat atcgttgata aattcaaaga aggcggcaac gaaaaagtga
    27361 ttctttgcga tcgcggtgct aacttcggct atgacaacct ggttgtcgat atgctgggct
    27421 tcagcatcat gaagaaagtg tctggtaatt cgccggtgat tttcgacgtt acccacgcac
    27481 tgcaatgtcg cgatccattt ggcgcagctt ccggcggtcg ccgtgctcag gtggctgagc
    27541 tggcaagagc cggtatggcg gtaggtctgg cggggctgtt tattgaagcg catccggatc
    27601 cggaacatgc gaaatgtgat ggtccatccg cgctgccgct ggctaaactg gaaccgttcc
    27661 tcaagcaaat gaaagcgatt gatgatctgg tgaaaggttt cgaagaactg gataccagca
    27721 agtaatcttt tttgcctgaa aaataaagtc ttagcgttct gcgttaagac ttttttcatg
    27781 ggtgccggat acaaaaaagg ccgcaggctg ttacccctgc ggccggtttc gggcgcatat
    27841 tgccatcacg gcagcctgac gcccgttttc accttacttc cggttacgcc accagctgac
    27901 aatcactgcg gtaataatcc ccgccaggat cggtgccgcc aggttgtgcc agaaaatcat
    27961 ggcaaactgc gcgagcgtca tatagccgcc ttgttgtaat gacaacattt tgcggctatt
    28021 cttgaattgt tctggttcaa gattagcccc cgttctgttg tcaggttgta cctctcaacg
    28081 tgcgggggtt ttctctttcc agcaaccaat gccaccaggg ataaagcccc cgcaacattg
    28141 cgcctcaccg gataacgccg gcttggtgtg gatactacgt cgcaattcat cttcacttca
    28201 tccctgaaat gtttgcaata aagagtacat tccggttttt caacagctgt tgcagtcatt
    28261 tcgtgcaggt tgtggtacag gcttgcaatt ctgattaaca acctgtccag ctagtatgaa
    28321 gaaatataat aagcaggaat ttatattatc tcccccgatt tccgtcatcg gaggagatat
    28381 aactgtcagg caaatatcgt catcaaatag gcggcaaaca gtgccagatg cgctgcgcca
    28441 ttgagcacgt tagttcgtcc ggtggagaag gagatatggc acagtactaa agaggccacc
    28501 atcaccacca tttctggcgc accaagtgca aactgcaatt cgttacccgt cataaaggca
    28561 attagcgtga cgacaggtac ggtaagcgaa atggttgcca acactgaacc aaagaacaga
    28621 ttcatcgcgc gctggacctg gttattcaac actgctttta acgcacctaa accttccggc
    28681 gacagaatca acagagccac caggaagcca gtaaaggcga caggcgcatt catgctgtcg
    28741 agcaatgtct ccagcgggct ggcgttcatt ttggtgactg caataacggc aatcagatgg
    28801 acaatcaacc agatggcatg ccacaggctg ctatgggcag acggtttacc gtggtgcggg
    28861 tcgtcatcat cactgtcatc ttcgtgctcg tagacaaaca aactttgatg cgttttggtc
    28921 tggatcagca aaaatacgcc atacattgcc gcagaaatta atgctaccag taacgcctga
    28981 cctgttgaaa aattcgccgc aggcagagcc attggaaata ccagtacgat tatcgccagg
    29041 gggaacagcg caattaaata ctgcttgata ccaaacagat tcatatattg ggtggcaaac
    29101 ttacgtccgc ccaacaataa tgaaaagcca accagcccac ctgtaacaat cataatgatt
    29161 gaatagagcg tatcacgcat tagcgttggc gcggcatcgc cggttgccat taaagctgaa
    29221 atcaaactga cttcaagaat aaccactgaa aggctaagaa taagcgaacc gtaaggttct
    29281 cccaggcgat gggctaatac gtccgcatga cggacaacac taaaggcgct acttaaaata
    29341 ccaataagcg caagaagatt gatggcaatg accactggta gtgtctggct gcttccccac
    29401 aggaacagca ctaccagcgc cagaaccggg aaaataagcg aagtctcctt gtggcgggtt
    29461 tttaccgcct cttgagcatt tgacattatg gttatccctt tgcagatgaa tttatcgaaa
    29521 atgtaaaaaa taggtaggaa aaataacaga aattgtctgg atatcggtaa cattttacga
    29581 atttttaccc cgctgtcggt tttttactca tttgggcata aaaattagta atacgtttag
    29641 acaatgtttg ttttagcact tcttaagaag agtctgacat gaaaattctt atgttttggc
    29701 aagagtagat attgttgacc acacttaatg ttcaactttg taaaaggagt caacgatgtc
    29761 gtataaaacg aaaagcgatc tgccggaaag cgtaaagcac gtgcttccgt ctcatgccca
    29821 ggatatctat aaagaagcgt tcaacagcgc atgggatcaa tataaagata aagaagatcg
    29881 gcgtgatgac gccagtcgcg aagaaacagc gcataaagtg gcctgggctg ctgtgaagca
    29941 tgaatatgcc aaaggggatg atgataaatg gcataaaaaa tcgtcaaacc ggtagcttag
    30001 ttaaagctat tcgtgcggtg ttgccttgca agtggtccgt ggattgcata ttgtcccgtt
    30061 agtggtttca aaatgagcag taaaaatgtc cggaagacac caaaaagttg tcgcagggaa
    30121 gtatgcagtg gcggaggtgt aaggtgataa cgcgtgattt cttgatgaat gccgattgta
    30181 aaacggcatt tggtgccatt gaagaatcac tcttatggtc agcagaacaa cgggcggctt
    30241 cgctggcggc gacgctggct tgtcgacctg atgagggacc ggtgtggatc ttcggttatg
    30301 gatcgttgat gtggaatccg gcactggagt ttaccgagtc gtgcaccggt acactggttg
    30361 gatggcatcg cgcgttttgc ctgcgtctga ccgccgggcg cgggactgcg caccagccgg
    30421 gacgaatgct tgcactgaaa gagggcggac gcaccacagg cgtcgcctat cgactgccag
    30481 aagagacgct ggagcaggaa ctaaccctgc tgtggaagcg tgagatgatt accggctgtt
    30541 atctgccaac ctggtgtcag cttgatcttg atgatggacg cacagtaaac gccattgtgt
    30601 ttattatgga cccgcgacat ccagaatatg aatctgacac tcgcgctcag gtcatagcgc
    30661 cgttgattgc cgcggcgagc ggtccgctgg gaaccaatgc acaatacctg ttttcactgg
    30721 aacaggagct tatcaaactg ggaatgcagg acgatgggct gaatgatttg ctggtatcgg
    30781 taaaaaaact gctggcggag aattatccgg atggtgtgtt acggccggga ttcgcctgag
    30841 taaacttccc tcatagtggg gcgtccgacg ccccctcaaa cattaaaatg tgagcacttt
    30901 atcggctgac agcgtccatt gcgccagttc aacaagagta ccgatttcca ccccatcaat
    30961 cagaggaagt gtactaatcc cgcgcccgtc ggtacaggtt ttgcacaatt tcaccggtac
    31021 attctgagcg gtaaggatct ccagcatttg ctgaatgttg tagccttccc ctggtttttg
    31081 cccgcgcaac ccggctgtga ccgcatcaga catcaggaac agacgcagat ccagattgtt
    31141 ctcctgctct cgtaacgcaa tggccagccg caagctgtta aacaaggatt cgctcccgta
    31201 aggtgcgcca ttggcaacga tcacgatttt ttgcattatt tactcctgta ttcagggaat
    31261 tagacactca tcttctatct tactgcttct gcagcgtctg accaatcggt cacattttta
    31321 aggattttcc tgaaagcgcg agaaaatacg acaaaaggtg ccagtaatcg ttattcttta
    31381 aggctatggt ttttcatttt taccggaagt taccgacgtt ttgagccgtt tcgttcctcg
    31441 cattattccg ttttatttac tcttgctggc ggcaggcggt acagctaacg cacaatctac
    31501 cttcgagcaa aaagcggcaa atcactttga gaatagcaat gatggtctgc cggatttagg
    31561 catggcacct gaacatcatg atggggaaaa acactttgct gaaattgtga aagatttcgg
    31621 cgaaaccagt atgaatgata acgggctgga tactggcgag caggcaaaag ctttcgcatt
    31681 gggaaaagtc cgcgatgcgc ttagtcaaca ggttaatcag cacgtagagt cctggctatc
    31741 accgtgggga aatgccagtg ttgacgtcaa agtggataac gaaggacatt tcaccggcag
    31801 tcgtggaagc tggtttgtgc cgttacaaga taatgatcgt tatctcacct ggagccagct
    31861 tggtcttact cagcaggatg atgggctggt gagcaatgtg ggcgttgggc aacgctgggc
    31921 gcgcggcagc tggctggtgg gttataacac tttttatgac aacttgctgg acgaaaatct
    31981 tcagcgagcg ggctttggcg ctgaagcgtg gggcgaatat ttgcgactat cggcaaactt
    32041 ttatcagccg tttgctgcat ggcatgaaca gactgccacg caggaacaac ggatggcgcg
    32101 cgggtacgac ctgacagccc ggatgcgcat gccgttctat caacacctca ataccagtgt
    32161 cagcgtagaa cagtattttg gtgatcgtgt cgatttgttt aactctggta cgggttatca
    32221 caatcccgtc gcgttgagtc tgggattaaa ttacacccct gtgcccttag tcactgtgac
    32281 ggcccagcat aaacagggtg aaagtggcga gaatcaaaat aacctcgggc tgaatcttaa
    32341 ctaccgcttt ggtgtaccgc tcaaaaaaca actttctgcg ggcgaggttg tcgaaagtca
    32401 gtcgttacgt ggtagtcgct atgacaatcc gcagcgaaat aatcttccga ctcttgagta
    32461 ccgacagcga aaaacgttaa cggtgtttct ggcgacaccg ccgtgggatc taaaacctgg
    32521 cgaaacagtg ccgctgaaat tacaaatccg cagtcgttac ggtattcggc aactgatttg
    32581 gcagggcgat acgcagatat taagtttgac gccgggcgca caagccaaca gtgaggaggg
    32641 ctggacgctg atcatgcctg actggcagaa cggggaaggg gcgagcaatc actggcgatt
    32701 gtctgtagtg gtggaagata accaggggca gcgtgtctcc tccaatgaga tcacgctaac
    32761 gcttgtcgaa ccgttcgacg cattgtcaaa cgacgaactg cgctgggaac cgtaatcaga
    32821 aaatgcgctc ctgatgcacc cataccgcag cttccacgcg agactttagc ttcattttct
    32881 tcagcatgtg cttgacgtga acttttactg tgctttcggt gatgtccagg cggcgggcaa
    32941 tcatcttgtt cggcaaaccc tgggcaatca gcttgagaat atcgcgctcg cgtggggtta
    33001 actggttaac atcgcgctca gtagtggcac ggttagcgcg caagctggcg gccagaacag
    33061 gcgttaatgc ttcgcttaat accatttcgc cagcagctgc ctgatgtaac gctttcagca
    33121 gatcttccgg ttccatatct tttaacagat agccatccgc gccgcgtttc agtgcggtga
    33181 ccacatcttc ttcatggtta gagacgctga ataccacaat gcgcccggag agggactttt
    33241 cgcgcagttt atccagcgtt tccagaccgt tcatgccggg catattgaga tctaacagga
    33301 tcagatcggg atcaagagac tccgccagtt caataccctg ttcgccatta ctcgcttcgc
    33361 caaccacagt gatatctggt gccatactga taagctgttt tacgccagtt cgcaacatcg
    33421 ggtgatcgtc gatcagcagg atagtagccg gttcctgatt actcatgggt atctccttgg
    33481 acgtctgtga aagttttttc gggaataaag gtgaccacca cttcggtgcc acctgattca
    33541 cgacggcgga cgcggcaatc gcctcgtaaa ctttgcgcac ggtcgcgcat tattatcatg
    33601 ccgtagtgat tgctgcggat ggcattttca ggcacgccgc agccgttatc ctggacggtc
    33661 agtttgacct gattatcgtt ttgcgccacc gtcaccacga cctcactcgc ttgcgaatgt
    33721 ttgagggcgt tacttaatgc ctcacgggca atttgcaaca agtggattgc ctgatgcgaa
    33781 ggcaccagac gaggcggcaa ttgataatcc agcttcaccg ggaagccaaa tttggcgctg
    33841 tactcttcgc aactcgcctc cagcgccgga cgtaatccag gctcggtgag ctgcaaacgg
    33901 aatgtggtga gcaattcacg caactgcacc caggatgcat tcagttcgtt acggatctga
    33961 cttaacagtt cgcggctgct ttctggcagc gcatcgccct gcatctgtaa gcaactcacc
    34021 tgcatcttca tgcaagagag agattgggca atagaatcat gcagttcgcg cgcaatggtg
    34081 gcacgctctt ccatcacgat caactgttgc tgacgttcct gatggcgatc cagcgccagc
    34141 gtggcggtga gttgttcaac cagggtatcc accagttgtt gttgatcatg gctaagatga
    34201 cgcccctgcg gcagggtcgc cagcaaaata ccgtactgcg tatgagtgtc agccagccgc
    34261 cacttcaggg ttgtgccgcg atcgccaacg ggtaatacgc cgcgcgggca gagctggcag
    34321 cctttatcat cacaagtcat atctgactgg caggtaaact cctgatgatt ctcttcatca
    34381 tcagtgtcat acacccgcag ttcgatatca cgtagcaggg ttaaattctg taagccgttg
    34441 agtacaggtg acaggcgttc acacagcggg gcgcgggaat gcaaacggcg gttagcctgc
    34501 cataaaaaag agaggatctg atttttatgc tccagcccag cggttttctc ctgaacccgc
    34561 tgctcaagta cggcataact ttccgccagt tctgcagaca tattgttcaa cgcagttcca
    34621 agcatcgcca tttcgttgcg cccgctgatg tttgcgcgtt gggtaaaatc gcgatggctg
    34681 acggcactcg ccattgccag cagttgccgc cacggttgta gcaatctcgc ccgcaaccag
    34741 ataatagtga ataccagtaa aagtgccata aataccgcca ttacccgatg gaccagcacc
    34801 actgtctcaa tgcgcatttc cgtggtgcga tcaaaaccag ataccagctg atcaagcccg
    34861 gcaacaaact ggctgacatc cgctgacacc gtttcgcggt tttgtgcacg tatcagtgca
    34921 gggatcagtt cattacgcca gtaatcttgt aaaccctgta attgcgccag ttgcccgtct
    34981 cgttctgctg ctcgagtcaa ctcggcgcta aatgctgttt gttccatctc tttaattaag
    35041 ggcttgtctt tctcgcttaa tggcactgct gccaacagac ggtaactttg catgcgcagc
    35101 gatcccgctt tgttgatcgc atgggcgctg ccctgaacgc cttgcaccag ccagccagaa
    35161 accgccatcc ctgccagtcc aatagcagta gaaagcaaca caataagcgc aacctgatta
    35221 accagggtga gcggagagag acaacgttta agcatgtaaa cctcttcctt caggctttaa
    35281 atgagcaata accttaatga atgtgacgat acattctgga atggcagtat tctcggctat
    35341 tggctgaagt atacccatac ccggaaagag ttactcctta tttgccgtgt ggttagtcgc
    35401 tttacatcgg taagggtagg gattttacag taccgtgaaa aatctcataa tttttatgaa
    35461 atcactgtac tcactatgga taatgataaa tatcaatgat agataaagtt atcttatcgt
    35521 ttgatttaca tcaaattgcc tttagctaca gacactaagg tggcagacat cgaaacgagt
    35581 atcagaggtg tctatgagtc actcatccgc ccccgaaagg gctactggag ctgtcattac
    35641 agattggcga ccggaagatc ctgcgttctg gcaacaacgc ggtcaacgta ttgccagccg
    35701 caacctgtgg atttccgttc cctgtctgtt gctggcgttt tgcgtatgga tgttgttcag
    35761 cgctgttgcg gtgaacttac cgaaagtcgg ttttaatttt acgaccgatc agctatttat
    35821 gttgactgcg ctgccttcgg tttctggcgc gttattacgt gttccatact cctttatggt
    35881 tcctatcttt ggtggtcgtc gctggacggc gttcagcacc ggtattctga ttattccttg
    35941 cgtctggctg ggttttgccg tgcaggatac ctccacgcct tatagcgtct tcatcatcat
    36001 ttctctgctg tgcggctttg ctggcgcgaa cttcgcatcc agtatggcaa acatcagctt
    36061 cttctttccg aaacagaagc agggtggcgc gctgggtctg aatggtggtc tgggcaacat
    36121 gggcgtcagc gtcatgcagt tggttgctcc gctggtggta tcactgtcga ttttcgcagt
    36181 atttggtagc cagggcgtca aacagccgga tgggactgag ctgtatctgg cgaatgcgtc
    36241 ctggatatgg gtgccgttcc ttgccatctt caccattgcg gcgtggtttg gcatgaacga
    36301 tcttgctacc tcgaaagcct ccatcaagga gcagttgccg gtactcaaac ggggtcatct
    36361 gtggattatg agccttctgt atctggcaac cttcggctcc ttcatcggct tctccgcggg
    36421 ctttgcgatg ctgtcaaaaa cgcagttccc ggatgttcag attctgcaat acgctttctt
    36481 cgggccgttt attggtgcgc tggcgcgttc tgcaggtggt gcattatctg accgtctggg
    36541 cggaactcgt gtcacgctgg tgaactttat cctgatggcg attttcagcg gcctgctgtt
    36601 cctgacctta ccgactgacg gacagggcgg aagcttcatg gcgttcttcg cagtcttcct
    36661 tgcgctgttc ctgaccgctg ggctgggtag tggttccact ttccagatga tttccgtgat
    36721 cttccgtaaa ctgacaatgg atcgcgtgaa agcagaaggg ggttctgacg aacgtgcgat
    36781 gcgtgaagcg gcaaccgaca cggcggcggc gctgggtttc atctctgcga ttggcgcgat
    36841 tggtggcttc tttatcccga aagcgtttgg tagctcgctg gcattaacgg gttcgccagt
    36901 cggcgcaatg aaagtatttt tgattttcta tatcgcctgc gtggtgatta cctgggcggt
    36961 atatggtcgg cattctaaaa aataaatcgt aatatttgat tatcctttgc gcggcatgat
    37021 gtcgcgcttt ttttatgtgt catttagtta caacatacta atgttgtatg gcttatttcg
    37081 ctggatttca ttaagagtca ttaatatgtt acccatgggg aatactcctt aatacccatc
    37141 tgcataaaaa tcttaatggt ttaaataacc acagg
//
LOCUS       DACADF010000028        36735 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.028, whole genome shotgun sequence.
ACCESSION   DACADF010000028 DACADF010000000
VERSION     DACADF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.028"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(264..2585)
                     /locus_tag="ISB30_001380"
     CDS             complement(264..2585)
                     /locus_tag="ISB30_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP1989027.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGILQLSLNADPQFEEPPKESYCEQIIMEHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREVQPAGNSAMMDALAAAMGKKR"
     gene            complement(2682..3194)
                     /gene="greB"
                     /locus_tag="ISB30_001381"
     CDS             complement(2682..3194)
                     /gene="greB"
                     /locus_tag="ISB30_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAP1989028.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASW
                     YVNAIEYVKP"
     gene            3386..4105
                     /gene="ompR"
                     /locus_tag="ISB30_001382"
     CDS             3386..4105
                     /gene="ompR"
                     /locus_tag="ISB30_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAP1989029.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            4102..5454
                     /gene="envZ"
                     /locus_tag="ISB30_001383"
     CDS             4102..5454
                     /gene="envZ"
                     /locus_tag="ISB30_001383"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAP1989030.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5531..7153)
                     /gene="pckA"
                     /locus_tag="ISB30_001384"
     CDS             complement(5531..7153)
                     /gene="pckA"
                     /locus_tag="ISB30_001384"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP1989031.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7531..9255
                     /locus_tag="ISB30_001385"
     CDS             7531..9255
                     /locus_tag="ISB30_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAP1989032.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQNRLWGWLALVFIATLGMSGLLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLDLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSARKEWDALDMSLLPRKITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9353..9550)
                     /locus_tag="ISB30_001386"
                     /pseudo
     CDS             complement(9353..9550)
                     /locus_tag="ISB30_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9558..10766
                     /gene="tnpB"
                     /locus_tag="ISB30_001387"
     CDS             9558..10766
                     /gene="tnpB"
                     /locus_tag="ISB30_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP1989033.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEHKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(10889..11767)
                     /gene="hslO"
                     /locus_tag="ISB30_001388"
     CDS             complement(10889..11767)
                     /gene="hslO"
                     /locus_tag="ISB30_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP1989034.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(11792..12193)
                     /gene="hslR"
                     /locus_tag="ISB30_001389"
     CDS             complement(11792..12193)
                     /gene="hslR"
                     /locus_tag="ISB30_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAP1989035.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAVLLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(12204..12872)
                     /gene="yrfG"
                     /locus_tag="ISB30_001390"
     CDS             complement(12204..12872)
                     /gene="yrfG"
                     /locus_tag="ISB30_001390"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAP1989036.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(12937..15072)
                     /gene="igaA"
                     /locus_tag="ISB30_001391"
     CDS             complement(12937..15072)
                     /gene="igaA"
                     /locus_tag="ISB30_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAP1989037.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTSEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQEIR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMLFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            15392..15952
                     /gene="nudE"
                     /locus_tag="ISB30_001392"
     CDS             15392..15952
                     /gene="nudE"
                     /locus_tag="ISB30_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAP1989038.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(16118..18670)
                     /gene="mrcA"
                     /locus_tag="ISB30_001393"
     CDS             complement(16118..18670)
                     /gene="mrcA"
                     /locus_tag="ISB30_001393"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAP1989039.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVDDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            18790..19569
                     /gene="hofM"
                     /locus_tag="ISB30_001394"
     CDS             18790..19569
                     /gene="hofM"
                     /locus_tag="ISB30_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAP1989040.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            19569..20108
                     /locus_tag="ISB30_001395"
     CDS             19569..20108
                     /locus_tag="ISB30_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HAP1989041.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLTRSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            20092..20532
                     /gene="hofO"
                     /locus_tag="ISB30_001396"
     CDS             20092..20532
                     /gene="hofO"
                     /locus_tag="ISB30_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAP1989042.1"
                     /translation="MNMFFDWWFATSLRLRQLCWAVWLLVLVTLIFLSSTHQEERDAL
                     IRLRASHHQQWAALYRLVDATPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            20522..20926
                     /gene="hofP"
                     /locus_tag="ISB30_001397"
     CDS             20522..20926
                     /gene="hofP"
                     /locus_tag="ISB30_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAP1989043.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            20838..22076
                     /gene="hofQ"
                     /locus_tag="ISB30_001398"
     CDS             20838..22076
                     /gene="hofQ"
                     /locus_tag="ISB30_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAP1989044.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHIGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            22477..22998
                     /gene="aroK"
                     /locus_tag="ISB30_001399"
     CDS             22477..22998
                     /gene="aroK"
                     /locus_tag="ISB30_001399"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAP1989045.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            23055..24143
                     /gene="aroB"
                     /locus_tag="ISB30_001400"
     CDS             23055..24143
                     /gene="aroB"
                     /locus_tag="ISB30_001400"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP1989046.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHSIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGRFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            24235..25521
                     /gene="damX"
                     /locus_tag="ISB30_001401"
     CDS             24235..25521
                     /gene="damX"
                     /locus_tag="ISB30_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAP1989047.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            25628..26464
                     /gene="dam"
                     /locus_tag="ISB30_001402"
     CDS             25628..26464
                     /gene="dam"
                     /locus_tag="ISB30_001402"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAP1989048.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            26482..27159
                     /gene="rpe"
                     /locus_tag="ISB30_001403"
     CDS             26482..27159
                     /gene="rpe"
                     /locus_tag="ISB30_001403"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP1989049.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            27152..27910
                     /gene="gph"
                     /locus_tag="ISB30_001404"
     CDS             27152..27910
                     /gene="gph"
                     /locus_tag="ISB30_001404"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAP1989050.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            27903..28907
                     /gene="trpS"
                     /locus_tag="ISB30_001405"
     CDS             27903..28907
                     /gene="trpS"
                     /locus_tag="ISB30_001405"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP1989051.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            29069..29974
                     /locus_tag="ISB30_001406"
     CDS             29069..29974
                     /locus_tag="ISB30_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989052.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            29991..30353
                     /locus_tag="ISB30_001407"
     CDS             29991..30353
                     /locus_tag="ISB30_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP1989053.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            30437..31600
                     /locus_tag="ISB30_001408"
     CDS             30437..31600
                     /locus_tag="ISB30_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAP1989054.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVSDAVEQGTDVITVFTLDKAREISAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLVEYGVTHTEPGHALTGTIPANQRGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHHGEPEIVGRYDSLGNPLEA"
     gene            31601..32827
                     /locus_tag="ISB30_001409"
     CDS             31601..32827
                     /locus_tag="ISB30_001409"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP1989055.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLETLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKMGRIVREQVQVGRVITFGGLLTDSQCILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLNEFNTYPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSFRFAGDTL"
     gene            32824..33702
                     /locus_tag="ISB30_001410"
     CDS             32824..33702
                     /locus_tag="ISB30_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAP1989056.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVGCTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITSLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            33713..34066
                     /locus_tag="ISB30_001411"
     CDS             33713..34066
                     /locus_tag="ISB30_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAP1989057.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            34078..35382
                     /locus_tag="ISB30_001412"
     CDS             34078..35382
                     /locus_tag="ISB30_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989058.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGILILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            35394..36479
                     /locus_tag="ISB30_001413"
     CDS             35394..36479
                     /locus_tag="ISB30_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP1989059.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKSGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRGGEETILRILRESIASI"
     gene            complement(36587..>36735)
                     /locus_tag="ISB30_001414"
     CDS             complement(36587..>36735)
                     /locus_tag="ISB30_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAP1989060.1"
                     /translation="ALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIGGCG
                     PTAQ"
BASE COUNT         8554 a         9205 c        10083 g         8893 t
ORIGIN      
        1 taatctgact caatgttgat atggctcaag cttatatcgc agcgaaaata taattcgcat
       61 gacgaattat tagggctggt taatgtttaa ttaaggtttc agttgttaat gaagtgataa
      121 atattaacgg gatatattat gcgtttgctt gtggaataaa aaggccggag atattctccg
      181 gcacttttat gtcaggcaag gcgttcgttg ccagatgtgg cgtgaacgcc taatcgcgca
      241 ggtttagggg ccagtccggg cgtttaacgt tttttgccca ttgccgccgc cagcgcatcc
      301 atcatcgcgc tgttaccggc aggctgcact tcacgcccac gcggtttggc tgccgggcgg
      361 ttgttttgcg ggcgttcatt accgccgccg cgacgggcgt tggtttcgcc aggctgctcg
      421 tccagacgca tggtcagggc gatacgttta cgctgaagat ccacttccag cactttcacc
      481 ttcacaatgt cgcccgcttt caccacggta tgcggatctt ccacaaactt gttcgacaat
      541 gaagagatgt gaaccaggcc gtcctgatgc acgccaatat cgacaaacgc gccaaagttg
      601 gtgacgttgg tcaccgcacc ttcgaggatc atacccggtt gcaggtcgtt cattgtctcg
      661 acaccatcgg caaactgagc ggttttaaat tccggacgcg gatcgcgacc cggtttttcc
      721 agctctttga tgatgtcggt taccgtcggc acaccgaatt tctcatcagt aaagtcagac
      781 gctttcaggt tacgcagttc gctgctgtta cccatcagat ctttcagtgc ctgctgtgtt
      841 gctgccagaa tgcgttccac caccggatag gcttccgggt gaacggtaga agcgtccagt
      901 gggttatcac cgtggttaat gcgcaagaag cccgcgcact gctcgaaggc tttcggcccc
      961 agacggctca cttttaacag ttgctgacgg ttctggaact gaccgttctc atcgcgccag
     1021 gctacgatgt tttgcgccat catgcgcgtc aggcccgcca cgcgggttaa cagaggaaca
     1081 gaagcggtgt tgagatcgac gccaacggcg tttacgcagt cttctactac tgcatccagt
     1141 ttgcgtgcca gctgcgtctg gctgacgtca tgctgatact gacctacgcc gatagatttc
     1201 ggatcgattt tcaccagctc cgccagcgga tcctgcaaac gacgggcgat agacaccgcg
     1261 ccacgcagcg aaacgtcgag atccgggaat tcctgtgctg ccagctcgga agccgagtaa
     1321 accgacgcgc cagcttcgct gacgatcact ttctgcgcgg tcactttcgg gaactgcttc
     1381 tgcacgtcga gatagaaacg ttcggtttcg cgggaagctg taccgttgcc gatcgccacc
     1441 agttcaacgt tatgcttttc acacagcgca gcaacggtca tcgctgcttt tgcggcctgc
     1501 ccggtatgcg gataaatggt atcggtcgcc accagtttac cagttgcatc gactaccgcc
     1561 acttttaccc cggtacgcag acccggatcg aggcccatcg ttgcacgcag tccggcaggg
     1621 gccgccatca gcagatcgtg caggttacgg gcaaagacgt tgattgcttc atcttccgcg
     1681 cgttcgcgca cggtgcccat cagttcagtt tccagatgca tcaacacctt gatgcgccac
     1741 gtccagctca ccacaccttt gcgccagcta tccgccgggg cattgttcag gcgcaggcca
     1801 aggtgttcca tgatgatttg ctcgcaatag ctctctttcg gcggctcttc gaactgcgga
     1861 tcggcattca gcgaaagctg aagtatgcct tcgttacgac cacggaacat cgccagtgcg
     1921 cggtgagaag gcaccgttga cagtggttcg tgatgatcga aatagtcgcg gaattttgcc
     1981 ccttcctctt ctttaccgct caccacggtt gagaccagat gcgcgttctt ccacagataa
     2041 tcacgcactt tcgccagcag cgcggcatct tcggcaaacc gttccatcag gatatagcgc
     2101 gcgccgtcca gcgcggcttt ggtatctgcc acgcctttat cggcatcaat atattgtgca
     2161 gcggcgactt ctggcgtgtg tgacggatcg ctccacagca gatcggccaa cggctcaagc
     2221 cctgcttcaa tggcgatttg cccacgggtg cggcgtttag gtttgtaggg caggtagagg
     2281 tcttcgagtt cggttttgct tagggtggcg ttgatggcat tcgccagatc atcggtgagt
     2341 ttgccttgct cggaaataga cttgaggatc gcctgacgtc tctcttccag ctcgcgcaga
     2401 tagctcagac gcgtttccag attacgcagc tgcgtgtcat ccagaccgcc ggtgatttcc
     2461 ttacgataac gtgcgataaa cggcacggta ttcccttcgt caagcaggcg aacggcagcg
     2521 tcaacctgtt ccgggcgcgc ctgaatttca cccgcaataa tgcggcagaa cgaatcattc
     2581 atcatggttt gagttcatct tttcggatca aaaatcaggg gatagttata cggactggct
     2641 ggcaaaaatg ccagccatcg gcaggaggtt aagactcttc cttacggttt cacgtactcg
     2701 atagcattaa cataccagct cgcttccccg gcaggggtat tcaccaccgc cagatcgccg
     2761 acttcttttt tcagcaatgc gcgggccatc ggggaatcga tagagatgta atctttacgg
     2821 ccaaaaattt catcgtagcc gacaatacgg aaacggtgag tcacaccatc gtcgttttca
     2881 atctccaccc acgcaccaaa aaagactttg ccttcctgct gcggggagta atcgacgatt
     2941 ttgagatttt ccatgcattt agtgagatag cgcacgcgac ggtcgatttc acgcaggcgc
     3001 tttttattat actgatagtc agcattttcg ctgcggtcgc ccagacttgc ggcccaggtc
     3061 actttctttg tgacctccgg gcgttcttca cgccagagat aattaagctc ttgtttgagt
     3121 ttttcatacc cttcccgggt aaccaggggc gttttcatct cgttgattcc ctttgtctgt
     3181 ttgataatgc gcacattggg tataacgtga tcatatcaac agaatcaata atgtttcgcc
     3241 gaataaattg tatacttaag ctgctgttta atatgctttg taacaattta ggctgaaatt
     3301 cataccagat ttagctggtg acgaacgtga gcttttttaa gaatacacgc ttacaaattg
     3361 ttgcgaacct ttgggagtac aaacaatgca agagaactac aagattctgg tggttgatga
     3421 cgacatgcgc ctgcgcgcgc tgctggaacg ttatctcacc gaacaaggct tccaggttcg
     3481 aagcgtcgct aatgcagaac agatggatcg cctgctgact cgtgaatctt tccatcttat
     3541 ggtactggat ttgatgttac ctggtgaaga tggcttgtcg atttgccgac gtcttcgtag
     3601 ccagagtaac ccgatgccga tcattatggt gacggcgaaa ggggaagaag tggaccgtat
     3661 cgttggcctg gagattggcg ctgacgatta cattccaaaa ccgtttaacc cgcgtgaact
     3721 gctggcccgc attcgtgcgg tgctgcgtcg tcaggcgaac gaactgccag gcgcaccgtc
     3781 acaggaagag gcggtaattg ctttcggtaa gttcaaactt aacctcggta cgcgcgaaat
     3841 gttccgcgaa gacgagccga tgccgctcac cagtggtgag tttgcggtac tgaaggcact
     3901 ggtcagccat ccgcgtgagc cgctctcccg cgataagctg atgaaccttg cccgtggtcg
     3961 tgaatattcc gcaatggaac gctccatcga cgtgcagatt tcgcgtctgc gccgcatggt
     4021 ggaagaagat ccggcgcatc cgcgttacat tcagaccgtc tggggtctgg gctacgtctt
     4081 tgtaccggac ggttctaaag catgaggcga ttgcgcttct cgccacgaag ttcatttgcc
     4141 cgtacgttat tgctcatcgt caccttgctg tttgtcagcc tggtgacgac ttatctggtg
     4201 gtgctgaact tcgcgatttt gccgagcctc cagcagttta ataaagtcct cgcgtacgaa
     4261 gtgcgtatgt tgatgaccga caaactgcaa ctggaggacg gcacgcagtt ggttgtgcct
     4321 cccgctttcc gtcgggagat ctaccgtgag ctggggatct ctctctattc caacgaagcc
     4381 gccgaagagg ccgggctgcg ttgggcgcaa cactatgaat tcttaagcca tcagatggcg
     4441 cagcaactgg gcggtccgac ggaagttcgc gtcgaggtta acaaaagttc gcctgtcgtc
     4501 tggctgaaaa cctggttgtc tcccaatatc tgggtacgcg tgccgctaac cgaaattcat
     4561 cagggcgatt tctccccgct gttccgttat acgttggcga ttatgctatt ggcgataggc
     4621 ggggcgtggc tgtttattcg tatccagaac cgaccgttgg tcgatctcga acacgcagcc
     4681 ttgcaggtgg gtaaagggat tattccgccg ccgctgcgtg aatatggcgc ttcggaggtg
     4741 cgttccgtta cccgcgcctt taatcatatg gcggctggtg ttaagcaact ggcggatgac
     4801 cgcacgctgc tgatggcggg ggtaagccat gatctgcgca cgccgctgac gcgtattcgc
     4861 ctggcaaccg agatgatgag cgagcaggat ggctacctgg cagaatcgat caataaagat
     4921 atcgaagagt gcaacgccat cattgagcag tttatcgact acctgcgcac cgggcaggag
     4981 atgccgatgg aaatggcgga tctcaacgca gtactcggtg aggttattgc tgccgaaagt
     5041 ggctatgagc gggaaattga aactgcgctt taccccggca gcattgaggt gaaaatgcac
     5101 ccgctgtcga tcaaacgcgc ggtagcgaat atggtggtca acgccgcccg ttacggcaat
     5161 ggctggatca aagtcagcag cggcacggag ccgaatcgcg cctggttcca ggtagaagat
     5221 gacggtccgg gtattgcgcc ggaacaacgt aagcacctgt tccagccgtt tgttcgtggc
     5281 gacagtgcgc gcactattag cggcacggga ttagggctgg cgattgtgca gcgtatcgtg
     5341 gataaccata acgggatgct ggagcttggc accagcgaac ggggcgggct ttccattcgc
     5401 gcctggctgc cagtgccggt aacgcgggcg cagggcacga caaaagaagg gtaaataaaa
     5461 cgggaggcga aggtgcctcc cgttttgctt tctataagat actggataga tattctccag
     5521 cttcaaatca ttacagcttc ggaccagccg ctaccagcgc ggcacccgca ggggtgtcgg
     5581 tgtatttatc gaagttgtcg ataaacagtt tcgccagcgt ttcggctttt tcctgccact
     5641 gttccggaga agcgtaggtg ttacgcggat cgaggatctt cgtgtctacg cccggcagtt
     5701 cggttgggat cgccaggtta aacatcggca gagtgaaggt ttctgcgtta tccagcgaac
     5761 cgttgaggat ggcgtcgata atagcgcggg tatctttaat ggagatacgt ttgccagtgc
     5821 cgttccagcc ggtgttaacc agatacgcct gcgcacccgc tgcctgcata cgtttcacca
     5881 gcacttctgc gtattgggtc gggtgcagcg acaggaacgc cgcgccgaag caagcggaga
     5941 aggttggcgt cggttcggtg atgccgcgct ctgtaccagc cagtttggcg gtgaagccag
     6001 agaggaagtg gtactgggtt tgatcggcag tcagacgaga aaccggcggc agcacaccaa
     6061 aggcgtctgc tgtcaggaaa ataactttag ttgcgtggcc cgctttggaa accggcttaa
     6121 cgatgttatc gatgtgatag atcggataag aaacgcgggt gttctcggtt tttgaaccat
     6181 catcaaagtc gattgtgcca tcttcacgca cggtgacgtt ttccagcagc gcatcgcgac
     6241 ggatagcgtt gtagatttca ggttccgctt ctttcgacag cttgatagtt ttcgcgtagc
     6301 agccgccttc gaagttaaac acaccgtcat catcccagcc gtgctcgtca tcgccaatca
     6361 ggcggcgttt cgggtcggtg gaaagggtgg ttttaccggt gccggaaagg ccgaagaaca
     6421 ccgcaacatc gcctttctcg ccaacgttgg cggagcagtg catagaagcg atacctttca
     6481 gcggcagcag gtagttcatc atcgagaaca tccctttctt catttcgccg ccgtaccagg
     6541 tgccgccaat cagctgcatg cgctcggtca ggttaaacgc cacgaagttt tcggagttca
     6601 gaccctgttc tttccactgc gggttagtgc acttcgcgcc gttcataacg ataaagtctg
     6661 gtttgaaacc tgccagctct tcatcgctcg ggcgaataaa catgtttttg acaaaatgcg
     6721 cctgccaggc cacttcggtg atgaaacgga cggaaagacg agtatccggg ttcgcaccac
     6781 agaaggcgtc gacaacgaac agacgtttgc cggaaagctg cctggtcacc aggcctttca
     6841 gatgctgcca ggtttccgga gagagaggtt tgttgtcgtt cttacctttg cctttgtctg
     6901 cccaccagaa agtatcgcga gtggtatcgt cacgaacgat atacttatct ttcggtgaac
     6961 gaccggtgaa gatcccggta tcgacggcaa cggcacccag attagttaac accccgcgct
     7021 cataaccggt caggctcggg tccagctctt cctgatacag cagatcgtag cttgggttgt
     7081 aaacgatatc atgtacgtca ctgataccat aagcctcgag ttcttgcggg gtcaaaccat
     7141 tgttaacgcg catttcactg ctccttagcc aatatgtatt gcctgaatag taaagtcttt
     7201 ttgggggtgt taaccgcgac aaggctcata gatttacgta tctggagaaa ttcatatttg
     7261 acaaaaaact ccgtgattcc tgtcacgaaa cggttgctat tatcgcagaa atagcatttt
     7321 aggtgtggaa aatgttctca aaaggttaaa tcttggttta tatggcggga atattgtgag
     7381 tggaaccgca ttcatgaatg aaattgatgc aaattcaatc ggtaaagtat cgattcacca
     7441 cgctaacttt ttttgattat tttctgaaaa aagcaaaata ctactgcccg atgcgataat
     7501 taacccacct tttgattctg gataaccaac atggataacg ttgaactttc accggcaaca
     7561 cgctggggga tgattgctac cggattactt caggggctgg tttgctacct gctgatcgcc
     7621 tggcttgccg gaaaaaatct cagttggatt gtttatggcg tgcctgcgac cgtcgcgttt
     7681 tcatccgttt tactcttctc cgtgatctcc tttaaacaga atcgcctttg gggatggctg
     7741 gcgctggtgt ttattgccac gctgggaatg agcggcttgc tgaagtggca aactgatggc
     7801 atgaccccct ggagagctga aaaggcgctt tgggattttg gctgctatct gctgctgatg
     7861 gcaatgttgt tgctaccgtg gatacaacaa agccttcgca tccgcaatga cagtagccgc
     7921 tatcgctact tttaccagtc agtatggcat aacgtactta tattactggt gatttttctc
     7981 gccaatggcc taacgtggct ggtactttta ctgtggagtg agttgtttaa acttgttggc
     8041 atcacgtttt tcaaaacgct cttttttgca accgactggt ttatttatct cacgttaggt
     8101 ctggttaccg cgctggcggt gatcctcgcg cgaacacagt cacgtttaat cgactctatt
     8161 caaaagttgt tcacgttaat cgccacgggg ttgctgccgt tagtatcatt gctaaccctg
     8221 atgtttatta tcaccctgcc gtttacgggc ctgagcgcga tttctcgcca catctccgcc
     8281 gccgggttgc tgttgacgct ggcctttttg caattgatct taatggctat tgtccgcgat
     8341 ccgcaaaaag cgtcacttcc ctggacgggg ccattgcgtt gcctgattaa aaccgctttg
     8401 ctggttgctc cgctgtatgt gttcgtcgcc gcctgggcgt tatggctgcg ggtcgctcag
     8461 tacggctgga ctgtcgaccg cttgcagggc gcgctggcgg tgctggtgtt actggtatgg
     8521 tcgctggggt attttgtcag tatcgtctgg cgtaaagggc aaaatcccct tgatcttcag
     8581 ggcaaagtga accttgcggt ttcgttattg gtgttggtaa tactggtgct tcttaattcg
     8641 ccggtgctgg acagtatgcg cattagcgtg aacagtcata tggcgcgtta tcagagcggt
     8701 aaaaacacgc cagaccaggt aactatctac atgctcgaac agagcggtcg ctatggacgt
     8761 gcggcgcttg agtcgctgaa aagcgatgcc gagtatatga aagacccgaa acgcgcgcgg
     8821 gatctgctga tggcgttaga tggagagcaa catcttcagg aacaggtatc ggaaaaagta
     8881 ttagccgata atgtgttaat tgccccaggt tctggtaaac cggatgcgac attctggtcg
     8941 gccttaatcc aggatcgcta taacgtgatg acctgtattg aaaaagacgc ctgcgtactg
     9001 gtcgagcaag atctgaatag tgatggtcag gcggagcgga tcctgtttgc ttttaatgat
     9061 gacagagtca ttgtctatgg ctttgactca gccagaaaag aatgggacgc gcttgatatg
     9121 agtttacttc cgcgcaaaat aacgaaagaa aaattactca cagctgcgaa ggatggaaaa
     9181 ctggggacga ggccgaaagc gtggcgcgat ctgacggtag atggtgaaac gctggaaata
     9241 aacttaagta agtgacaacg cttttaacgg atacgacgct gtatctgacc aaaataactt
     9301 gcgatttatc cggtgcgcgg taaaaccccg tccttcaggg cggggatata agttcgccat
     9361 ccatttcaac ccgttcagct tcaaaatcag cacatacatt tgaaaagtaa gtgcgtagtt
     9421 tttctgtcgc gtcatggtca aaaatctggc gttggtattt ggcgacaaag accaggtgaa
     9481 catgcatcag gaaaacacaa tgccttccac gccggatatc ggtttctttt ttcatagacc
     9541 aaaatataat ttgacctgtg aaacgactac aggcatttaa attccagtta agacccggtg
     9601 gtcaacagga gcgtgaaatg aggcgcttcg caggcgcatg tcgtttcgtt ttcaatcgtg
     9661 ctctggcgct tcagaatgag aatcatgagg ccggtaataa atacatccct tacgggaaaa
     9721 tggcttcctg gctggttgag tggaaaaatg ccactgaaac gcaatggctt aaagattctc
     9781 cctcacagcc attgcaacag tcactgaaag accttgagcg ggcttacaaa aacttcttcc
     9841 agaagcgggc tgcttttccc cgattcaaaa agcggggaca gaatgatgca ttccgctacc
     9901 cgcagggcgt taagctcgat caggaaaaca gccgtatttt tctgccgaaa cttggttgga
     9961 tgcgctaccg gaatagccgt caggtcacgg gagttgtgaa aaatgtcact gtcagccagt
    10021 cctgcggtaa gtggtacatc agtattcaga cagaaagtga agtatcaact ccggttcacc
    10081 cttcagcatc aatggtcggg ctggatgctg gcgtggctaa actcgccacg ctgtcagatg
    10141 gcacagtctt tgagcctgta aacagttttc agaaaaacca gaagacgctg gcgagacttc
    10201 agcgacagtt aagccgcaag gtcaaattca gcaacaactg gcagaagcag aaacgcaaaa
    10261 tacagcggct gcattcctgt atcgcaaata tccgcaggga ctaccttcac aaagtcacaa
    10321 cgaccgtcag caaaaaccac gcaatgatag tcattgagga tttgaaggtc agcaacatgt
    10381 caaagtcagc agcgggtacg gtcagccagc cggggcgcaa tgtccgggca aaatcaggtt
    10441 taaaccgttc gatactggat cagggctggt atgaaatgcg ccgccagctt gagcacaagc
    10501 agctctggag tggcggtcag gtgcttgctg ttccgccagc gtacacaagc cagcgttgcg
    10561 cgtgctgtgg tcatacagcg aaagaaaacc gcctgtcaca aagtaaattc agatgccagg
    10621 tatgtggata tacagcgaac gccgatgtaa atggcgctcg taacatttta gcggcggggc
    10681 acgccgttct tgcctgtgga gagatggtgc agtcaggccg cccgttgaag caggaaccca
    10741 ccgaaatgat tcaggcgaca gcctgaacgt agcagggatc cacgtccttc agggcgtgga
    10801 ggatgtcaat tcataggcct ggtaagcgca gcgcatcagg ccattacgtc gtaaaatccg
    10861 gcagagtcct ctctgccgga catactcatt aatgaacttg cggatctgcc ggagacgcgt
    10921 tgttgcggat ctcggcaata tccatcgcat tgaacagata gtggttaccg cagtaatcac
    10981 aatgcatgtc aatttcgcca tcttccgcca ggatgctatc gacttcttca tcaggcagcg
    11041 ttttcagcgc atcggcgcaa cgttcacgcg agcaggtgca tttgaactcc acatcctgcg
    11101 gatcgtaaac cgtcacctct tcttcgtgat acaaacgcca caacacttcg tttgccggta
    11161 aagtcagcag ttcttcggtt ttgatggttt cggttagcgt cgccaggtgg tcaaagtcgt
    11221 cctgctgggc attttgcgca ggcattacct gcaacaacat accgcctgca gccggtttgc
    11281 cgtctacgtc gccggtgcga ataaacaggc gcgtcggcag ctgttcagaa cgcataaagt
    11341 aatcttccag gcaggccgcc agggtatcac cttccagacc aaccacgccc tgatagcgtt
    11401 cgccttcgct cggggtgatg gtgatcacca cgtaaccatt gccgaccagc gttttcaggt
    11461 cggcattttc tggaatttcg ccctgcacgc gcgccacacc gcgcatctgc tggttgttgt
    11521 taccgttaat aaccgccaga ttcatcggac cgtcgccttg cagctgtacg gtgatatcac
    11581 catcaaactt caacgtagcg gttaacaggc tggtcgcaac cagcagttct gccagcacgt
    11641 ttttaacggg ctgcggataa tcgtgattct caaggatctg ttgcagggtt tccgaaacgg
    11701 ttaccagttc gccgcgaacg gcaaagtttt caaacagata gcgatgtaat tggtcatgtt
    11761 gcggcataat catctctctt gcaggtgaca gttattcact gtcgccgtgt ttaaatcgta
    11821 acaggtcgcg gcgctctttt ttgtccgggc gtcggtccgg atgcggcatg gttaaggcat
    11881 taagtttacg tgccagcgcc attttttcgc gtttctctac actttccgca gtctcttcat
    11941 acagcaagac tgcctcgctg gcggggcgac gctgttcagt aatcgccttt acaatcaccg
    12001 tgcgttcgtc atttccctgg cgcagagtga gcgtggcatt cagctcgacg attttgctcg
    12061 gcttgctgcg ctgcccgttg taatgcacct taccaccttc gatcatttcg cgggccagcg
    12121 cgcgggtttt ataaaaacgg gcggcccata gccatttatc cagtcgaacc tcaacagcag
    12181 gtttttcttt catggcgtct ccttcacatt agcgagggga tcaggcggcg gtagtcattc
    12241 agtgacggat ggcgctgata ctgtttctcg gcaatcccgg agtcaggatt agtcacaccg
    12301 aggcagtaac gaataccaaa ttgcgcggca gcatcgagaa tcgcttcgct gtcatcaata
    12361 aacagcgttc tttcagcttt aagacccgta gcttcggcca ccgcatgcca taaccgctga
    12421 tcctctttcg gataaccaaa tgtgtgggtg gaaagtaata aatcaaggtg tgcgtccaga
    12481 ccggtatgct caagttttac cgccaggttg tgcggatgcg cattggtgag caaaattcgc
    12541 tgtttaccgc tggctttcag tgcctcaaga aacggaatgg tatcttcacg cagtacggca
    12601 cgcggtccca tctcggtggt catcgcacag atatccagac ccagttgctc actccagtaa
    12661 tcaagacagt accagtttag cgtatgctgt acgtcgtgat attgctggcg catatattcc
    12721 atcgcttcct gtggcgtaac tccgtttttc gcgccccatg tttcaggcac cagcttttgc
    12781 cagaaatagt tatcgaaggc gaggtcgagc aacgtgccgt ccatatccag cagaacggta
    12841 tctacgtcct gccaggcaat gttgatatgc atgagggaaa tctccagagt gaagcaattt
    12901 gcgcgacagg gtagcataac ctgccgcgca aacgtgttat tcgataaggc tttcggaagg
    12961 ggtgatcagt tgcgggttca ggcagctttc ataataggcc tgaatcttca tcatgcgagt
    13021 acgatgacgc tggtaacgac gccaggcctg tacgccatta taaatggcgc tacccagcat
    13081 cgtcagtagc agcagcgtgg tgctgagata gcgccacaga ccggaacgat ccgggatcgg
    13141 atgaaggcca atatgctgcg taccattggc gtcagtgaag atttttgtga cgataccttc
    13201 ggcgttaaac ggcgtatgca tcagcatttg tgccagtttc tggaaagcgt tccactgttc
    13261 ttgcggcgga tagtcgtaaa gtgatgccga aggccagggc tgatcaacaa aatcgctgcc
    13321 ttcgtcgctg acaatcagga acccgccggg ggccggactg tttagtgatt gtgccgctcg
    13381 cgccgtttca tgcgtgataa acggcgcggt agaggtggcc accaggttat ccagcgattc
    13441 cgcactcacc gggcgtaata acacattcac gccatcgagt ttcccggcgt tggcgcgttt
    13501 taccagcgcg tcccagtctt tactgttgcc gagattgacc agcgcatttt tcagtcgcac
    13561 acagtcatct ttggcagaac acagatccgc tgtcttcagt actatgtcgc caaaatcatc
    13621 aagcaatacc atgccggatt tttgaattgc tgaacgtaat gaggcgctga cgcgagattc
    13681 atcttccggt ttagggtgca gctggcgatt aactgcttcg gtcaatgccg tcgctttgtt
    13741 gaccaattca gattctggta atggcaatga gcgggcgtcg ttccagatga tctgcgagca
    13801 gtcaaacggt aaaaaaggtg agttggtttt cgcgctccag gtaccggaag tgcgaatatt
    13861 acacattccc gtaccgctaa tacgcaatgt atcgccaacc cgcacgccag cgtcagccag
    13921 ttgtttcacg ctggtggctt caatggtctg cgcgcctttc atccacgaga gggtgaattt
    13981 cagcggcata tccagcggga tccagaataa cagcataaac agcaccagta gcgagcccgc
    14041 agcgataatc gtactgcgca gccagtgctg taacggaaag ttttttactt catcatggag
    14101 cgaaagatat cgcccctgac gcactacgtg gcggtcgaga tagatatcga tatcggtttg
    14161 ttggccgaga tcctgagcaa tgtagggctg ccagtgtgcg ggatagacca ggtcgataat
    14221 accgagtgaa atattgttga tctgttcctg atcgttttcg ccaaacaatc cccaacgacg
    14281 gggtgtaccg cgcagacaat gaatttcccg cagggaggat tttgccgggg gcgcgaatag
    14341 cccccacaga cctgcgccca gcagcagtaa cgcgccgcct gccagccacg gaacaaagac
    14401 atccggggta atcaggcaga aaaagagcat caggaaggag gcgacaatca gcaacgcttc
    14461 gcgcagcccg cgcggacgac tcagcgcata ttcttcatgc gtttctttgc gaatattgag
    14521 cagctctatt tgctcgctct cttccccgcg aatcgacgcc tgcgttgacg gaacaggttg
    14581 caaggcatag ccgcgaattt cctgcatgta ctcctgcagc gtatgaccgt tgagtgaaat
    14641 aaccagcggc agcgaatcgg tatgaatcag ttcaacggta ttctcatcgt tgatgtactg
    14701 ttcccagaaa gggggcaggt ggacttctac cgaatcgagg tagtaacgcc atttattcgg
    14761 atcgtcggta gagatgccat aacgcgtgat agcgtgtgtc agcatcatga cgttgttgct
    14821 ttcggcattc agcgccagag agatcggtgc cgcgctggct cccgttgggc caggcacctg
    14881 taatacctgc gtcaggctct caagataatt ttcaacggcg ctacgttctt cagatgtgag
    14941 tttacgcgtt tgcgcatccg cgaaggcgtt ggtccagggc agctgacgcc gtctggatcg
    15001 cacttttatc agccatcccg caagtagtga gcaggccagc aaagcagcta aaaaaatcac
    15061 aatggtgctc atgctttccc catcttactt agtctgtcag gcgtggtcag tcgcaccgga
    15121 cacaatacat gattctgcgg ttggctatcg gcaagcgtgg agtttaactt gagcatcatt
    15181 aagcacatca cagtaaccgc tgatcatagc aaaccctctg aaggcggaat atcagcaaat
    15241 tcctggagtt atttcaacct attgactttt aatttgattt gaggacgatt atttattaag
    15301 ttacataaat gtaaataacg cgcaattcac attgccgaaa gcgtgtccga tatcgcacaa
    15361 taaccgctga tttcacagca tagatcccac gatgagcaaa tcattacaaa aacccaccat
    15421 tctgaatgtt gaaactgtag cccgttcccg actgtttacc gtcgaaagcg tggatctgga
    15481 gttcagcaat ggcgtgcggc gtgtttatga acgaatgcgt ccaaccaacc gggaagcagt
    15541 gatgattgtg ccgattgtgg acgatcacct gatcctgatt cgcgaatacg cagtgggaac
    15601 tgaatcctac gaattaggtt tttcgaaagg attaattgat ccgggtgaaa gcgtctttga
    15661 agccgctaac cgcgagctaa aagaagaggt tggatttggt gcgaacgatc tgactttttt
    15721 gaagaagctc agcatggcac cgtcttactt ttccagcaaa atgaatatcg tggtagcgca
    15781 agatctctac ccggaatcac tggaaggcga tgagccagag ccgttaccac aggtgcgctg
    15841 gccgctggcg catatgatgg atttgctgga agcccctgac ttcaacgaag cgcgtaatgt
    15901 cagcgcgctg ttcctcgtgc gcgaatggtt gaaagggcag gggcgagtgt aaatgcgtga
    15961 agtttacctg atgcataatg cttatcaggc ttacgaagtt cctgcaattt attgaatttt
    16021 caattttttg ttggccggat aacgcgttca cccgcatccg gcataaacaa gtgcactttg
    16081 tcagcaaact gaaaaaggcg ccgaagcgcc tttttaatca gaacaattcc tgtgcctcgc
    16141 cattatcgat aatggtcgtt cccacctcgt gcaccgcctg ttgtgtcggc tgcgtacctt
    16201 cgatgaaata ctcttcgcgg ctgttgccac cattagctaa ttgcccggtg ctgcgatcga
    16261 tattcaccgt cacaataccc ggtggcggcg tcagcggctg ttccggtaca ccttcaagaa
    16321 cggctttcat ataagcgtcc catgcaggct gggcactctt ggcaccgcct tcgtaacctg
    16381 agatctgatc tttaatcgct ccggaagccg ttgtatgacc gagattacga cggtgatcat
    16441 caaagccaat ccagaccgag gtcacaacgc ccggaccgta acccgagaac cacgcatctt
    16501 tcgaactgtt agtggtaccc gtttttccgc ctatatcatg ccgttgtaaa tcacgacctg
    16561 ctcgccagcc agtaccctgc cagcctggtt caccaaaaat attggtgttc aaggcactct
    16621 taatcaggaa tgccagcgga gtgttgatga cgtgcggtgc gtactcctgc gcgccggtct
    16681 tcgccactaa cgcctgattt gcctgctcca gctgcggcat tggtacagaa acattctgct
    16741 gctcgcggga gatagcgaca tcttcaacat cgttattttc cagcacgttc gatttctgcg
    16801 tatcaccgta aatcaccgga atatcgcatt ccgggcaggc tactttcggt ttcgcttcga
    16861 aaattacgcc gccctgatcg ttttcaattt tgctgataaa ccacgggtcc accaggaagc
    16921 cgccgttcgc catgaccgcg tagccgcgcg ccacctgcat tggggtgaag gaagctgaac
    16981 ccagcgccag cgattcggtg tggacaatgt tttgtgccgg gaagccgaag cgttgcagat
    17041 attctgcagc gtagtcgacg cccatcgccc gcattgcgcg taccatcacc acgtttttcg
    17101 actgacccag cccctgacgt aagcgaattg gaccggcata ttgcggtggt gagttcttcg
    17161 gctgccagtc agaaccggca cctgcatccc agcgggaaat tggcacatcg ttcaacatac
    17221 ttgccagcgt cagaccttta tccatcgccg cggtgtagag gaacggtttg atgttggaac
    17281 ccacctgacg cagtgcctga gtggctcggt taaacttgct ctgattgaaa tcaaagccac
    17341 cgaccagcgc cataacggca ccgttttgcg gattgatcga caccagcgcc gagttcactt
    17401 ccggcacttg cgccagccac catgcatcgt caacttgacg aacccagatt tgctgacccg
    17461 tttgcagaac atcggtcact ttacgcggcg tcggtccctg ctgagtatcc gaacggtaag
    17521 gacgcgccca gcgaacacct tccatactca atacgacggt cgacccgtct gccagcatcg
    17581 ccgttgcttc ctgaggattg gcgctggtga ctgcggcagg cagcagcgga ccataggtcg
    17641 gcagcgcctt cagcgtatcg gtaatcttgt tgttatccca cgccgattcg cccactttcc
    17701 acagcacatt tgccgggccg cgatagccgt ggcgcatgtc gtagtccagc acgttattac
    17761 gtaccgcctg ctgtgcggcc tgctgcactt tgcgggtgat ggtggtgtaa atgcgataac
    17821 cgtcttcata ggcactttcg ccataacggt tatacatctc ctggcgcacc atttcgctca
    17881 ggtatggcgc agagaaagca atctccggcg cgtgatagtt agcgttaatc gcctcagtgc
    17941 gtgtctgatc gaactgttgt tgggtgatat acccttcatc cagcatccgc gacagcacga
    18001 cgttacgccg cgcgacggca cgatccattg agtagagcgg gttgaaggtg gaaggcgctt
    18061 tcggcagccc ggctatcacc gccatttcgt tcaacgtcag ttggtcgaca gttttaccga
    18121 aatagacttg tgccgcagca ccgacaccat aggcgcggta accgaggtaa atcttgttca
    18181 gataaagctc gaggatctcg tctttcgtta gcaactgttc aatgcgaatc gcgaggaaga
    18241 cttccttaat cttgcgcatc agcgtgcgtt ctggactgag gaagaagttt ctcgccagct
    18301 gctgggtaat ggtactcgcc ccttgtgacg cgtgaccgga gaacagcgcc acgcttgccg
    18361 cacggaagat ccccaccggg tcaacgccgt gatgctcgta gaagcggctg tcttccgtcg
    18421 cgataaaggc tttcaccatc tccggcggga tttgatccaa cgtaaccgga atacgacgtt
    18481 tctcaccgta ttgagcaatc agctcgccat cggcgctgta aatctgcatc ggaatttgca
    18541 ggcgaacatc ttttaatgtc gccacatccg gcagttgtgg ctcgatgtag cggtataggc
    18601 cataaatcga gcctgctccc agcagaatgc aacagactgc aaggatcaaa aaatacttta
    18661 cgaacttcac tggaaatttc ccatttagtt tcatttgggc agtttataaa caaacgcgcg
    18721 gtagtataaa ggcaagccag acgcattgat atacccgtca gagtgacggg tgataaggag
    18781 atcatcacaa tggcatttaa gaactggcaa attggtttgc atttacaaca gcaagaagcg
    18841 gtagcggttg ctatcgtgcg gagcgcaaaa gaatgcttat tgcaccgctg gtggcggttg
    18901 ccactggaga acgacattat caaagatggg cggattgttg atgtgcagcg gctggctaac
    18961 acgttgttac cgtggagtcg cgaactgccg cagcgtcatc acattatgtt ggcgtttccc
    19021 gccagtcgca cattacagcg gtcatttcca cgcccgtcga tgtccctcgg tgagcgggag
    19081 caaatggcct ggctgtccgg gacgatggcc cgtgaactgg atatggatcc ggactccctg
    19141 cgcttcgatt acagcgaaga ctcactcagt cccgcctata acgtgactgc cgcgcaaagc
    19201 aaagagctgg caacgctgct tactctggca gaaaggttgc gtgttcatgt tagcgcgatc
    19261 actccggatg ccagtgcatt acagcgattc ctgccttttt taccttctca tcagcaatgt
    19321 ctggcctggc gtgataacga acagtggctg tgggcgacgc gctatagctg ggggcgcaaa
    19381 ctggcggtgg ggatgactag cgcgaaggag ctggcggcag cgttatctgt tgatcccgaa
    19441 agcgtcgcgc tatgtggcga aggcggattt gacccctggg aggcggtttc tgttcgtcag
    19501 ccgccgctac cgccaccagg tggagacttt gccatcgcgc tggggctggc gcttgggaag
    19561 gtgtactgat gaacccgcca attaattttt tgccctggcg acagcaacgc cggaccgctt
    19621 ttcttcgttt ctggttgctg atgttcgttg cgcctttgct gctggccgtc gggataacgc
    19681 taatactgcg tctgacaagc aatgccgaag cacgcgtaaa cgccgttttg cttcaggcgg
    19741 aacaacaact cacccgcagc ttacagataa cgaaaccacg tttgctggaa cgccagcagt
    19801 tacgcgaaca gcgtttgcaa cgtcagcgcc agcgacaatt tacccgcgac tggcaatctg
    19861 cgctggaagc actagcggcg cttttacccg aacatgcctg gctgacaacg ataagctggc
    19921 agcagggaac gctggagatc aaggggctta caacaagcat taccgcgtta aacgcgctgg
    19981 aaacgtcact ccgccaggat gcttcttttc atctcaatca gcggggggcc acgcagcagg
    20041 atgcgcaggg acgctggcaa tttgagtatc agttaacaag gaaggttagc gatgaacatg
    20101 ttctttgact ggtggttcgc cacatcactc cgcctccgcc agctttgctg ggcagtctgg
    20161 ttgctggtgt tagttacgct catttttctg tcatcgacac accaggaaga gcgcgacgca
    20221 ttaattcgac tacgggcgag tcatcaccag cagtgggctg cactgtatcg cctggtagac
    20281 gccactccct tcagcgaaga aaaaacgctg cccttttcgc cactggattt tcagttatcc
    20341 ggcgcgcaac tggtttcctg gcatccatcc gcgcagggtg gtgagttggc gttgaaaacg
    20401 ctgtgggaag cagtgccgtc ggcatttaca cggctggcag agcgcaacgt cagcgtgagc
    20461 cgtttttcgt taagcgtgga aggtgatgac cttttgttca cgctacaact ggagacgccg
    20521 catgagggtt aaacgctggt tgttggcagg tattgcattg tgccttttaa ccggtatgcg
    20581 tgaccctttt aaaccgccgg aagatctatg ccggattagc gaacttagcc agtggcgcta
    20641 tcaggggatg gtagggcgag gcgagcgcat catcggtgta ataaaagatg ggcaaaaaaa
    20701 atggcgacgg gtgcagcaaa acgatgtgct ggaaaacggc tggacgatct tacagctgac
    20761 gccagacgca ctaacgctgg gaaccgggac aaactgcgaa ccgccacact ggttgtggca
    20821 acggcaagga gatacaaatg aagcaatgga tagccgcact actgttgatg ctgatacccg
    20881 gcgtacaggc ggcaaagccg caaaaagtga cgctgatggt ggatgacgtt ccggtagctc
    20941 aggtgttgca ggcgctggct gaacaggaga agttgaacct ggtggtttcg ccagacgtca
    21001 gcggtacggt gtcgttacat ttaactgacg ttccctggaa gcaggcacta caaactgtag
    21061 tgaaaagcgc cggattgata acgcgccagg agggcaacat tctctcggtg cattccgttg
    21121 cctggcagaa tgacaatatc gcccgccagg aggcggagca gacgcgggcg caggcaaatc
    21181 tgccgctgga aaatcgcaat attactctgc aatacgccga cgccggagag ctggcgaaag
    21241 cgggggagaa gctactgagt gccaaaggga gtatgaccgt cgataaacgc accaatcgcc
    21301 ttttgctgcg agataacaaa acggcgttaa gcacgcttga acagtgggta tcgcaaatgg
    21361 atctgccggt cgggcaggtt gagctgtcgg cgcatattgt caccattaat gaaaaaagtt
    21421 tgcgtgagtt aggtgtgaaa tggacgctgg ccgatgcgca acaagctggt ggcgttgggc
    21481 aagtcaccac gcttggcagc gacctctccg tagcgacggc gacaacgcat atcggtttta
    21541 acattggacg catcaacgga cgtttactgg atcttgagct ttccgcgctc gaacaaaaac
    21601 agcagctgga tattatcgcc agtccgcgtc tgctggcctc acatcttcag cctgccagca
    21661 ttaaacaggg gagcgaaatt ccatatcagg tttccagcgg ggaaagtggc gcgacgtcgg
    21721 tggaatttaa agaggccgtc ctggggatgg aagtcacgcc cacggtgtta caaaaaggtc
    21781 gcatccggct gaaattacac atcagccaga acgttccggg gcaggtgcta cagcaggcag
    21841 atggcgaagt gctggcgatt gataagcagg agatcgaaac gcaggtcgag gtcaaaagcg
    21901 gagaaacgtt ggcgctgggc ggcattttta cccgtaaaaa taaatcgggt caggatagcg
    21961 taccgttgct tggcgacatt ccctggttcg ggcaattatt tcgtcatgac ggaaaagaag
    22021 atgaacgacg cgagttagtg gtgtttatca cgccacgact ggtttccagt gagtaaacag
    22081 ccgtaaaagc ggtaatgttt ttacgctgaa cgtgtttcat ctatttgacg cgcgcaggta
    22141 tttagcatac aaggagtacc gatttgagag ttggtgctct tcgctgcctg cgttccatga
    22201 tgatgattta tcattcaggc ggcattttgc tgtctttttt acgctaatct tacccggtga
    22261 tttatcgcca gagcggtggt agcaaggcag cgcgcttgca gcgaccagat atgcagaggg
    22321 atgggtgatt tattcagttg ccaaacccgc tggagtattg agataatttt cagtctgact
    22381 ctcgcaatat cttatgaggt ttcagttcat gtcctgcggc gctctctgag cgaagcgggt
    22441 ttatcattaa cgaatagtct tagtagtacc gaaaaaatgg cagagaaacg caatatcttt
    22501 ctggttgggc ctatgggtgc cggaaaaagc actattgggc gccagttagc tcaacaactc
    22561 aatatggaat tttacgattc cgatcaagag attgagaaac gaaccggagc tgatgtgggc
    22621 tgggttttcg atttagaagg cgaagaaggc ttccgcgatc gcgaagaaaa ggtcatcaat
    22681 gagttgaccg agaaacaggg tattgtgctg gctactggcg gcggctctgt gaaatcccgt
    22741 gaaacgcgta accgtctttc cgctcgtggc gttgtcgttt atcttgaaac gaccatcgaa
    22801 aagcaacttg cacgcacgca gcgtgataaa aaacgcccgt tgctgcacgt tgaaacaccg
    22861 ccgcgtgaag ttctggaagc gttggccaat gaacgcaatc cgctgtatga agagattgcc
    22921 gacgtgacca ttcgtactga tgatcaaagc gctaaagtgg ttgcaaacca gattattcac
    22981 atgctggaaa gcaactaatt ctggctttat atacactcgt ctgcgggtac agtaattaag
    23041 gtggatgtcg cgttatggag aggattgtcg ttactctcgg ggaacgtagt tacccaatta
    23101 ccatcgcatc tggtttgttt aatgaaccag cttcattctt accgctgaaa tcgggcgagc
    23161 aggtcatgtt ggtcaccaac gaaaccctgg ctcctctgta tctcgataag gtccgcggcg
    23221 tacttgaaca ggcgggtgtt aacgtcgata gcgttatcct ccctgacggc gagcagtata
    23281 aaagcctggc tgtactcgat accgtcttta cggcattgtt acaaaagccg catggtcgcg
    23341 atactactct ggtggcgctt ggcggcggcg tagtgggtga tctgaccggc ttcgcggcgg
    23401 cgagttatca gcgcggtgta cgtttcattc aagtcccgac gacgttactg tcgcaggtcg
    23461 attcctccgt tggcggcaaa actgcggtca accatcccct cggtaaaaac atgattggcg
    23521 cgttctacca gcccgcttca gtggtggtgg atctcgactg tctgaaaacg cttcccccgc
    23581 gtgagttagc gtcggggctg gcagaagtca tcaaatacgg cattattctt gacggtgcgt
    23641 tttttaactg gctggaagag aatctggatg cgttgttgcg tctggacggt ccggcaatgg
    23701 cgtactgtat tcgccgttgt tgtgagctga aagcagaagt tgtcgccgcc gacgagcgcg
    23761 aaaccgggtt acgtgcttta ctgaatctgg gacacacctt tggtcattcc attgaagctg
    23821 aaatggggta tggcaattgg ttacatggtg aagcggtcgc tgcgggtatg gtgatggcgg
    23881 cgcggacgtc ggaacgtctc gggcggttta gttctgccga aacgcagcgt attataaccc
    23941 tgctcacgcg ggctgggtta ccggtcaatg ggccgcgcga aatgtccgcg caggcgtatt
    24001 taccgcatat gctgcgtgac aagaaagtcc ttgcgggaga gatgcgctta attcttccgt
    24061 tggcaattgg taagagtgaa gttcgcagcg gcgtttcgca cgagcttgtt cttaacgcca
    24121 ttgccgattg tcaatcagcg taacaacaag aaaggtcagg ccgcttatca agcggtctat
    24181 tagcttcagg ttaattgcaa cgtggtaagc attaaccttt tagtggggtg ttaaatggat
    24241 gaattcaaac cagaagacga gctgaaaccc gatcccagcg atcgtcgtac tggtcgttct
    24301 cgtcaatctt ctgaacgttc tgagcgtact gaacgtggcg aaccgcagat caattttgat
    24361 gatattgaac ttgatgacac tgacgatcgc cgtccgactc gtgcgcaaaa agcgcgtaat
    24421 gaggaaccgg aaatcgaaga agaaattgac gaatccgaag atgaaaccgt ggatgaagag
    24481 cgcgtagagc gtcgtccgcg taagcgtaaa aaagcagcca gtaaacccgc ttctcgtcag
    24541 tatatgatga tgggtgtcgg cattctggtt ctactgctgt tgatcatcgg tatcggttct
    24601 gcgctaaaag ccccctcgac ctcttccagc gatcaaactg cgtctggcga gaagagtatt
    24661 gatcttgcag gcaatgcgac cgatcaggcc aatggcgtgc agccagcgcc gggaaccacg
    24721 tctgcggaaa atactcagca ggatgtttct ctgccgccga tctcttctac gccgactcaa
    24781 gggcaaaccc cggcggcaac ggatggtcaa caacgtgttg aagtgcaggg tgacctgaac
    24841 aatgcgctga cccagccaca aaatcagcaa cagttgaaca atgtggcggt caattctaca
    24901 ttgccgaccg aacccgcaac ggttgcgcct gttcgcaatg gcaatgcatc gcgtgacacg
    24961 gcgaaaacgc aaaccgctga acgtccggcc actacgcgcc cagctcgtca gcaggcggtg
    25021 attgaaccga aaaaaccgca agcaaccgtg aaaacggaac cgaagccagt cgcacagacg
    25081 ccgaagcgaa ctgaaccagc tgcccctgtg gcgagcacaa aggcaccggc tgcgacttct
    25141 acgccagcac caaaagagac ggcgactacg gctccagtac agacggcatc cccggcgcaa
    25201 accacggcaa caccagccgc tggagggaag accgcaggta atgttggttc gttgaaatcg
    25261 gcaccgtcca gccattacac tctgcagctg agcagttcct ctaactacga caacctgaac
    25321 ggttgggcga agaaagagaa tctgaaaaac tacgttgtct atgaaacgac gcgtaatggt
    25381 cagccgtggt atgtcctggt ttctggcgtg tatgcttcga aagaagaggc gaaaaaagcg
    25441 gtatctacat tgccagcaga tgtccaggcc aaaaacccgt gggcgaaacc gctgcgtcag
    25501 gtacaggccg atctgaagta atcaaggtta tctcccgcaa tggtttatca ttgcgggagt
    25561 tgcctgaagc gctggatgct gtcggagctt tctccacagc cggagaaggt gtaattagtt
    25621 agtcagcatg aagaaaaatc gcgctttttt gaagtgggca gggggcaagt atcccctgct
    25681 tgatgatatt aaacggcatt tgcccaaggg cgaatgtctg gttgagcctt ttgtaggtgc
    25741 cgggtcggtg tttctcaaca ccgacttttc tcgttatatc cttgccgata tcaatagcga
    25801 cctgatcagt ctctataaca ttgtgaagat gcgtactgat gagtacgtac aggccgcacg
    25861 cgagctattt gttcccgaaa caaattgcgc cgaggtttac tatcagttcc gcgaagagtt
    25921 caacaaaagc caggatccgt tccgtcgggc ggtactgttt ttatatttga accgctacgg
    25981 ttacaacggc ctgtgtcgtt acaatctgcg cggtgagttt aacgtgccgt ttggccgcta
    26041 caaaaaaccc tatttcccgg aagcagagtt gtatcacttc gctgaaaaag cgcagaatgc
    26101 ctttttctat tgtgagtctt acgccgatag catggcgcgc gcagatgatg catctgtcgt
    26161 ctattgcgat ccgccttatg cgccgctgtc tgcgactgcc aactttacgg cgtatcacac
    26221 aaacagtttt acgcttgaac aacaagcgca tctggcggag atcgctgaag gtctggttga
    26281 tcgccatatt ccagtgctga tctccaatca cgatacgatg ttaacgcgtg agtggtatca
    26341 gcgcgcaaaa ttgcatgtcg tcaaagttcg acgcagtata agcagcaacg gcggcacacg
    26401 taaaaaggtg gacgaactgc tggctttgta caaaccagga gtcgtttcac ccgcgaaaaa
    26461 ataattctca aggagaagcg gatgaaacag tatttgattg ccccctcaat tctgtcggct
    26521 gattttgccc gcctgggtga agataccgca aaagccctgg cagcgggtgc agatgtcgtg
    26581 cattttgacg tcatggataa ccactatgtt cccaatctga cgattgggcc aatggtgctg
    26641 aaatccttgc gtaactacgg cattaccgcc cctatcgacg tacatctgat ggtgaaaccc
    26701 gtcgatcgca ttgtgcctga tttcgctgcc gctggtgcca gcatcattac ctttcatcca
    26761 gaagcctccg agcatgttga ccgcacgctg caactgatta aagaaaatgg ctgtaaagcg
    26821 ggtctggtat ttaacccggc gacaccattg agctatctgg attatgtaat ggacaagctg
    26881 gatgtgatcc tgctgatgtc tgttaaccct ggtttcggcg gtcagtcttt cattcctcaa
    26941 acactggata aactgcgcga agtacgtcgt cgtatcgacg agtctggctt tgacattcga
    27001 ctggaagtgg acggtggcgt gaaggtgaac aacattggcg aaatcgctgc ggcgggcgcg
    27061 gatatgtttg ttgctggttc ggcaatcttc gaccagccag actacaaaaa agtcattgat
    27121 gaaatgcgca gtgaactggc aaaggtaagt catgaataag tttgaagata ttcgcggcgt
    27181 cgcttttgat ctcgacggta cgctggttga cagtgcaccc ggtcttgctg ctgcggtaga
    27241 tatggcgctg tatgcgctgg agttgcctgt cgcaggtgaa gaacgcgtta ttacctggat
    27301 tggtaacggc gcagatattc tgatggagcg cgcattgacc tgggcgcgtc aggaacgtgc
    27361 gactctgcgt aaaacaatgg gtaaaccgcc cgttgatgac gacattccgg cagaagaaca
    27421 ggtacgtatt ctgcgtaaac tgttcgatcg ctactatggc gaggttgccg aagaggggac
    27481 gtttttgttc ccgcatgttg ccgatacgtt gggcgcgttg caggctaaag gcctgccgct
    27541 aggcctggtc accaacaaac cgacgccgtt cgtcgcgccg ctgctcgaag ccttagatat
    27601 cgccaaatac ttcagcgtgg tgattggtgg tgatgatgtg caaaacaaaa aaccgcatcc
    27661 ggacccgctg ttactggtgg ctgaacggat gggcattgct ccacaacaga tgctgtttgt
    27721 tggcgactca cgcaatgata ttcaggcagc aaaagcggca ggttgcccat cagttggctt
    27781 aacctacgga tataactacg gcgaggctat cgatctcagc cagcctgatg taatttatca
    27841 gtctataaat gaccttctgc ccgcattagg gcttccgcat agcgaaaatc aggaatcgaa
    27901 aaatgactaa gcccatcgtt tttagtggcg cacagccctc aggtgaattg accattggta
    27961 actacatggg tgcgctgcgt cagtgggtaa acatgcagga tgactaccat tgcatttact
    28021 gtatcgttga ccaacatgcg atcaccgtgc gccaggatgc acagaagctg cgtaaagcga
    28081 cgctggatac gctggcgttg tatctggctt gtggtatcga tcctgagaaa agcaccattt
    28141 ttgttcagtc ccacgtgccg gaacatgcgc agttaggctg ggcattgaac tgctatacct
    28201 acttcggcga actgagccgc atgacgcagt ttaaagataa atctgcgcgt tatgccgaga
    28261 acatcaacgc tggtctgttt gactatccgg tgctgatggc agcggacatc ctgctgtatc
    28321 aaacaaatct ggtaccggtg ggtgaagacc agaaacagca cctggaactg agccgcgata
    28381 tcgcccagcg tttcaacgcg ctgtacggcg atatctttaa ggtgccagag ccgtttattc
    28441 cgaaatctgg cgcgcgcgta atgtcgctgc tggagccgac caagaaaatg tccaagtctg
    28501 acgataaccg caataacgtt atcggcctgc tggaagatcc gaaatcggta gtgaagaaaa
    28561 tcaaacgcgc ggtcactgac tccgacgagc cgccagtagt tcgctacgat gtgcagaaca
    28621 aagcgggcgt ttccaacctg ctggatatcc tttctgcggt aacgggccag agcatcccgg
    28681 aactggaaaa acagttcgaa ggcaagatgt atggtcatct gaaaggtgaa gtggctgatg
    28741 ccgtttccgg tatgctgact gaattgcagg aacgttacca ccgtttccgc aacgatgaag
    28801 ccttcctgca acaggtgatg aaagacggcg cagaaaaagc cagcgcacac gcttcccgta
    28861 cgctaaaagc ggtgtacgaa gcgattggtt ttgtggcgaa gccgtaattt aacattaatg
    28921 ccaaaaaaac cggggaaacc cggttttttt atcctcattt gcaataatcc gaaaaaatgt
    28981 gaagcgcctc gccgtttcca catctcgctg ttgcgcctta tcttcaaatt catgaagata
    29041 taaacattca ttttattgaa aatatattat gcgtcgaacg tttatcaaaa aagaaggcgt
    29101 cgttatcacg acactggccc gttatttgtt gggtgaaaag tgcggtaatc gactgaaaac
    29161 catagatgag ctggcaactg aatgccgttc atcggttggc ctgacgcagg ccgcgttgaa
    29221 aacgctggaa tcaagcggag cgatacggat tgaacgccgt gggcgcaatg gcagttatct
    29281 ggttgagatg gataacaaag cattgctgac tcatgtggat atcaacaacg tggtatgtgc
    29341 gatgcccttg ccctataccc gtttgtacga aggcctggcg agcggattga aagcccagtt
    29401 tgatggcatt cctttttact atgcgcatat gcgcggcgcg gatattcgcg tggagtgttt
    29461 acttaacggc gtgtatgaca tggcggtggt ttcgcgactg gcggcggaaa gttatctcac
    29521 gcaaaaagga ttatgcctcg cgctggagtt gggaccgcat acctacgttg gcgagcacca
    29581 gttgatttgc cgtaaaggcg agtccgcaaa cgtgaagcgc gtggggctgg ataaccgttc
    29641 ggcggatcag aaaatcatga ccgatgtctt ttttggcgat agtgatgtgg aacgagtcga
    29701 tctctcttat cacgagagtt tacaacgcat tgttaaaggc gatgttgacg cggttatctg
    29761 gaacgtagtg gcggaaaacg aactgaccat gctgggatta gaagcgacgc cgctcacaga
    29821 cgatccgcga tttttacagg ccaccgaagc cgtggtcctg acgcgagtcg atgattaccc
    29881 aatgcaacaa ctgctgcgtg cagttgttga taaacatgcc ctgcttgctc atcaacagcg
    29941 agtagtcagt ggggaacagg aacccagtta ttaaacgaaa ggcatctgat atggaaacca
    30001 gactcaacct gctttgcgat gcaggcgtta ttgataagga catctgcaaa ggcatgatgc
    30061 aggtcgtcaa tgtactggaa acagagtgcc atctaccggt gcgcagtgag caaggaacga
    30121 tggcgatgac acatatggcg agtgcactga tgcgcagtcg ccgtggtgaa gaaatagagc
    30181 cgctggatga cgaattgctg gcggaactgg cgcaatccag ccactggcaa gccgttgtgc
    30241 aattgcatca ggtattgttg aaggaatttg cactggaagt taacccgtgt gaagaaggct
    30301 atttgctggc caacctttat ggattatgga tggctgctaa cgaagaggtt tgaagctccc
    30361 gataaatcca cgtcgtacaa cctaaatatt caaaaaaatg aatatttaat tcaaggaaca
    30421 gaaacgaggc aacaagatgt ttgtagaagc attgaaacgc cagaacccgg cgctgatttc
    30481 cgccgcacta agtttgtggc agcagggcaa gatcgccccg gacagttggg tgatcgacgt
    30541 ggatcaagtt ctggaaaacg gcaagcggct gattgagacg gcgcggcttt acggcattga
    30601 actgtatctg atgaccaaac aattcggtcg taatccgtgg ctggcagaaa aactgctggc
    30661 tctgggctac agcggcattg tggcggtgga ttacaaagag gcgcgagtca tgcgccgcgc
    30721 cggtttgccg gtagcgcatc aggggcatct ggtacaaatc ccttgtcatc aggtttctga
    30781 cgccgttgaa cagggaaccg acgtcatcac tgtgtttacc ctcgacaaag cgcgggaaat
    30841 ttctgcggca gcggtgaagg ccggacgtgt gcagtctgtg ctgctcaaag tttatagcga
    30901 cgatgatttt ctttatccgg ggcaggagag cggttttgta cagcattcgc tgcacgaggt
    30961 ggttgctgaa atccagaatc tgccggggct acatttggcg ggacttaccc atttcccttg
    31021 cctgctttgg gatgaggctg ccgggaaagt tttgccgaca ccgaatcttc acacgctggt
    31081 acaagcacgg gatcaactgg cgaaatctgg tattgcgatt gagcaactga acgcgccttc
    31141 agcgaccagc tgcacgtcgc tgccattgct ggtggaatac ggtgtgactc ataccgaacc
    31201 cggtcatgcg ctgacgggca ctattccggc aaaccagcgg ggcgatcagc ctgaacgtat
    31261 cgcgatgctc tggttaagtg aaatctccca tcatttccgt ggcgacagct actgctacgg
    31321 cggcggttac taccgtcgtg gtcacgcgca acatgcgctg gtgtttacgc cagaaaatca
    31381 aagaattact gaaacatatc tcaacgccgt ggatgacagc agtatcgact acaccctgcc
    31441 gctggcaggc gagcatccgg taagcagtgc agtggtgctc tgttttcgca cgcagatttt
    31501 tatcacccgt agcgatgtgg tgttggtgtc cggtattcac catggcgaac cagaaatagt
    31561 tggtcgttat gacagcctcg gcaaccctct ggaggcgtaa atggcgcgat ttgtggtgtt
    31621 agtgattgat agctttggcg taggggcaat gaaagatgtc acgttggtgc gtccgcagga
    31681 tgccggggcg aatacctgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac
    31741 gctggagacg ctggggctaa tcaacgcatt gggttatgcg cctggcgata tgcagccgtc
    31801 agactccgca acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg
    31861 gcatcaggaa attttaggta cgcgcccatt accgccgctg cggatgccct ttcgtgatgt
    31921 gattgaccgt gttgagcagg cattagtttc cgctggctgg caggttgagc gtcgtggcga
    31981 tgatctgcaa tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga
    32041 tttaggtcag gtctataaca tcaccgccaa tctctctgtg atctcttttg acgacgcaat
    32101 caaaatgggt cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg
    32161 cctgttaacc gacagccagt gcatcctcga tgccgcagaa agcaaagaag ggcgctttat
    32221 tggtatcaat gcgccgcgtt ctggcgccta tgacaacggt ttccaggtcg tacatatggg
    32281 ctacggtgtc gatgaaaaag tgcaggtgcc gcaaaaactg tatgaagcgg gcgtgccaac
    32341 cgtgctggtg ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa
    32401 cctggtggat agccagcgga ttatggatat caccctcaac gaatttaaca cctatccgac
    32461 ggcgtttatt tgtaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc
    32521 acgttatgcc gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgaggcgat
    32581 gcagccagat gattgcctgg tcgtgatggc ggatcacggt aacgatccga ccatcggtca
    32641 cagccaccat acccgcgaag tggtgccagt gctggtttat cagcaaggac tggtcgctac
    32701 gcagctcggt gtgcgcacca cgctttctga tgtgggggct accgtgtgtg aatttttccg
    32761 tgcgccaccg ccacaaaatg gtcgctcttt tctttcctcc ttccggtttg caggagacac
    32821 cctatgagtt ttgatccgac gggctacacc ctcgcccatg agcatctgca tattgatctt
    32881 tccggcttta aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag
    32941 atgaacgacc tgatggcccg gggcgtgcgt aatgtgattg agatgaccaa ccgttacatg
    33001 gggcgcaatg cgcaatttat gctcgatgta atgcgcgaaa ccgggatcaa cgtggtgggc
    33061 tgtaccggtt attaccagga cgcgtttttc ccggaacatg tggcgacccg cagcgtgcag
    33121 gaactggcgc aggagatggt cgatgaaatt gagcagggta tcgatggcac tgacctgaaa
    33181 gccggaatta tcgcggagat cggctccagc gaagggaaga ttacgtcgct ggaagagaag
    33241 gtgtttattg ccgccgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg
    33301 tcgttcagca cgatggggct ggagcaactg gctctgttgc aagcccacgg agttgatctt
    33361 tcgcgcgtca ccgtcggcca ctgcgatctg aaagacaatc tcgacaacat tttgaagatg
    33421 atcgatctcg gcgcgtatgt gcagttcgac accatcggca agaacagtta ctacccggac
    33481 gaaaagcgca ttgcgatgct ccatgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg
    33541 ctgtcgatgg atattacgcg gcgctctcat ttaaaagcca acggtggtta tggctatgac
    33601 tttttattga ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg
    33661 gatgtgatgt tacgtgaaaa tccctctcaa tttttccaat aaggacagac tcatgaaaaa
    33721 gattggcgtt gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctcc
    33781 tggctgtttt gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg
    33841 acaactggat tactacatcg gcgcgtgtaa taccggtgcg ggcgcagcct tgtcgattgc
    33901 tatcgcggtg attggctata acaaaagttg caccattgcc aagccgggca ttaaagcgaa
    33961 agacgaacat atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga
    34021 gcacgttgaa cacgcgattc cgatgctgat taaccatcta aaataaaagg cacgactatg
    34081 gatctgtata ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg
    34141 catcgctcgg cggccgtttt tcatgacggt atccgcccga tcctgccaca actgattgaa
    34201 ggctatatga accgtcgcga ggcggggagc atcgcttttg gtctgagcat tggttttgtg
    34261 gcctcggtgg ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt
    34321 cttcctaccg atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggt
    34381 gctatctggg gaatattgat cctcacctgt ctgttgccag taaaccagct gctgaccgcg
    34441 ctaccggtgg atgtattagg tagcctgggg gaattaagct cgccggtggt ttcagctttt
    34501 gcactgttcc cgttggtggc gattttctac cagtttggct ggaagcaaag tctgatcgcc
    34561 gccgtggtgg tactgatgac tcgtgtggtg gtagtacgct atttcccaca tcttaaccct
    34621 gaatccatcg aaatctttat tggcatggtg atgctgctgg ggatcgcaat aactcatgac
    34681 ctgcgtcatc gtgatgaaaa tgatatcgat gccagcggta tgtcggtgtt tgaagagcgt
    34741 acatcacgga ttatcaaaaa cttaccgtat atcgccatcg tgggtgcatt gattgctgcc
    34801 gttgccagca tgaagatttt cgccggtagt gaagtgtcga tcttcacact ggagaaagcg
    34861 tactccgcag gcgtaacgcc ggaacaatcg caaacactaa tcaaccaggc ggcactggca
    34921 gaatttatgc gcgggctggg ttttgtgccg ttgattgcca ccacggcgtt agccaccggc
    34981 gtgtatgcag ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg
    35041 atggttgcag cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttct
    35101 atcggcaaat ggttggggcg ctacccgtcg gtacgtaatg cgtcggataa catccgtaac
    35161 gccatgaata tgctgatgga agtggcgttg ctggtcggtt cgatctttgc agcaatcaaa
    35221 atggcgggct ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg
    35281 ggccgtccgg tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggcatcctg
    35341 ctgaatgttc tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga
    35401 cgtttcctct gcaaagcctg acgcttattg aggcgcagca aaagcagttt gcgctggtgg
    35461 acacgatttg ccgtcatttt cctggcagcg agtttcttac ctgcggtgat ttgggcttaa
    35521 cgccggggct aaatcagccc cgcattaccc agcgtgtgga acaggtgctg gctgacgcgt
    35581 ttcacgcaca ggccgcggcg ctggtacagg gcgcgggtac tggcgcgatc cgtgcggcgc
    35641 tggcagcttt gctcaaatcg gggcagcgtc ttctggtgca tgacgcgccg gtttatccaa
    35701 cgacgcgcgt cattattgag cagatggggc taacgctcat tacggctgat ttcaatgatc
    35761 tgtcggcact gaagcaggtt gttgacgagc aacaacctga tgccgcgctg gtgcagcata
    35821 cgcgccagca gccgcaggac cgctatgtgc tggcagacgt gctggcaacg ttgcgcgcgg
    35881 caggtgttcc agcgttaacc gatgacaact atgcggtgat gaaggtagcg cgaatcggct
    35941 gcgaatgcgg ggcgaatgtg tcgacatttt cctgcttcaa gctatttggg ccagagggcg
    36001 ttggtgcggt ggtaggcgat gccgatgtta ttagccggat tcgcgccacg ctctattccg
    36061 gcggcagtca gattcagggg gcgcaggcat tagaggtgtt gcgtggtctg gtgctcgcgc
    36121 cagtgatgca cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggcg
    36181 gcgcggtggc ggaagtgaaa agcgccgtca ttgccaatgc acagtcgaag gtattgattg
    36241 tggagtttca tcagccgatt gccgccagag tgctggaaga ggcgcagaaa ctcggtgcct
    36301 tgccttaccc ggttggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgacttt
    36361 ccggaacgtt tcgccaggcg aatccgcagt tagaacattg cgcgattcgc attaacccaa
    36421 atcgcggcgg tgaagagacg atattgcgga tcttgcgtga aagtattgcc agcatttaat
    36481 cgttgtaaac gatattttcg aaaagaatgc agaaacaaat acgccctcgg ataactccga
    36541 gggcgtcagg caattaaacc ggcgttagcc gatttctgtc agagacttac tgtgcagtcg
    36601 gaccgcaacc gccaatgatt ttagaaatag aaattgccgg gtgcagcagg tagtcgtaag
    36661 agcagttctt atcacggttt tcaatatgac cagtacatgc agttaacgta gccagtactg
    36721 cgcctaccag tgcaa
//
LOCUS       DACADF010000029        36536 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.029, whole genome shotgun sequence.
ACCESSION   DACADF010000029 DACADF010000000
VERSION     DACADF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.029"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(75..776)
                     /locus_tag="ISB30_001415"
     CDS             complement(75..776)
                     /locus_tag="ISB30_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HAP1989061.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSDGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELACMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGAMK"
     gene            complement(773..1702)
                     /locus_tag="ISB30_001416"
     CDS             complement(773..1702)
                     /locus_tag="ISB30_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP1989062.1"
                     /translation="MANTAEIFNFPVPDAAQKEPRVADLDDGYTRIANELLEAVMLAG
                     LTQHQLLVFLAVMRKTYGFNKKLDWVSNEQLSELTGILPHKCSAAKSVLVKRGIFIQS
                     GRNTGINNVVSEWSTLPESGKKNKVYLKEVNLPESGKKSLPKSGKGVYPNQVNTKDKL
                     TKDNIKPFSSENSGESSDQPENDLPVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTI
                     APSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWT
                     QLEINRNKQQAGVTASKPKLDLTNTDWIYGVDL"
     gene            complement(1789..2328)
                     /locus_tag="ISB30_001417"
     CDS             complement(1789..2328)
                     /locus_tag="ISB30_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HAP1989063.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYLEQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(2398..2628)
                     /locus_tag="ISB30_001418"
     CDS             complement(2398..2628)
                     /locus_tag="ISB30_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1989064.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            2667..3422
                     /locus_tag="ISB30_001419"
     CDS             2667..3422
                     /locus_tag="ISB30_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1989065.1"
                     /translation="MVVFSQQPFSFDGIKPWLYIWTMKTTLSERLKEARLARGLTQKA
                     LGDLVGVSQAAIQKIETGKANQTTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQST
                     VSHSKYFKIDVLDIEVSAGPGVINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINV
                     RGDSMSGTIEPGDLLFVDITVKSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKS
                     YSPWDPIEKDEMNRVFIFGKVIGSMPQTYRKHG"
     gene            4018..4224
                     /locus_tag="ISB30_001420"
     CDS             4018..4224
                     /locus_tag="ISB30_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HAP1989066.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            4300..4596
                     /locus_tag="ISB30_001421"
     CDS             4300..4596
                     /locus_tag="ISB30_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAP1989067.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRCGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHHVDIDSEV"
     gene            4602..5387
                     /gene="bet"
                     /locus_tag="ISB30_001422"
     CDS             4602..5387
                     /gene="bet"
                     /locus_tag="ISB30_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HAP1989068.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            5384..6064
                     /locus_tag="ISB30_001423"
     CDS             5384..6064
                     /locus_tag="ISB30_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HAP1989069.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            6061..6243
                     /locus_tag="ISB30_001424"
     CDS             6061..6243
                     /locus_tag="ISB30_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HAP1989070.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFHGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            6216..6407
                     /locus_tag="ISB30_001425"
     CDS             6216..6407
                     /locus_tag="ISB30_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HAP1989071.1"
                     /translation="MHKASPVELRTSIDLAHSLAQIGVRFVPIPAETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            6418..6699
                     /locus_tag="ISB30_001426"
     CDS             6418..6699
                     /locus_tag="ISB30_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP1989072.1"
                     /translation="MHFSGSGLHILCAYACRHGACSVTPQQENALRSIARQANSEIKK
                     VRQQFPDKNVNDICRSVLKKHRETVTLKGFTPTHLSLAIGMLNGVFKER"
     gene            6798..7019
                     /locus_tag="ISB30_001427"
     CDS             6798..7019
                     /locus_tag="ISB30_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HAP1989073.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYLAVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQQDLELIRKQRGIK"
     gene            7019..7345
                     /locus_tag="ISB30_001428"
     CDS             7019..7345
                     /locus_tag="ISB30_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP1989074.1"
                     /translation="MANLQLAVKSEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEVKTITHPHFGDKPVKVFAIKVNISTEYQSAQHKV
                     KNVQSD"
     gene            7329..7568
                     /locus_tag="ISB30_001429"
     CDS             7329..7568
                     /locus_tag="ISB30_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAP1989075.1"
                     /translation="MFRVIDPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRRK"
     gene            7708..7944
                     /locus_tag="ISB30_001430"
     CDS             7708..7944
                     /locus_tag="ISB30_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP1989076.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            7934..9076
                     /locus_tag="ISB30_001431"
     CDS             7934..9076
                     /locus_tag="ISB30_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1989077.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLGDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(9190..10440)
                     /gene="icd"
                     /locus_tag="ISB30_001432"
     CDS             complement(9190..10440)
                     /gene="icd"
                     /locus_tag="ISB30_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAP1989078.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            10612..11265
                     /gene="rluE"
                     /locus_tag="ISB30_001433"
     CDS             10612..11265
                     /gene="rluE"
                     /locus_tag="ISB30_001433"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAP1989079.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPDNQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVEEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWRDATD"
     gene            11275..11736
                     /gene="nudJ"
                     /locus_tag="ISB30_001434"
     CDS             11275..11736
                     /gene="nudJ"
                     /locus_tag="ISB30_001434"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAP1989080.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            11790..12896
                     /gene="mnmA"
                     /locus_tag="ISB30_001435"
     CDS             11790..12896
                     /gene="mnmA"
                     /locus_tag="ISB30_001435"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP1989081.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            12932..13573
                     /gene="hflD"
                     /locus_tag="ISB30_001436"
     CDS             12932..13573
                     /gene="hflD"
                     /locus_tag="ISB30_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAP1989082.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            13577..14947
                     /gene="purB"
                     /locus_tag="ISB30_001437"
     CDS             13577..14947
                     /gene="purB"
                     /locus_tag="ISB30_001437"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP1989083.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVADIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNHDHLLEELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            15116..15787
                     /gene="phoP"
                     /locus_tag="ISB30_001438"
     CDS             15116..15787
                     /gene="phoP"
                     /locus_tag="ISB30_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAP1989084.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            15787..17247
                     /gene="phoQ"
                     /locus_tag="ISB30_001439"
     CDS             15787..17247
                     /gene="phoQ"
                     /locus_tag="ISB30_001439"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAP1989085.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            17323..18444
                     /locus_tag="ISB30_001440"
     CDS             17323..18444
                     /locus_tag="ISB30_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP1989086.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(18493..19719)
                     /gene="pepT"
                     /locus_tag="ISB30_001441"
     CDS             complement(18493..19719)
                     /gene="pepT"
                     /locus_tag="ISB30_001441"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP1989087.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            19969..21105
                     /gene="potA"
                     /locus_tag="ISB30_001442"
     CDS             19969..21105
                     /gene="potA"
                     /locus_tag="ISB30_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAP1989088.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            21089..21946
                     /gene="potB"
                     /locus_tag="ISB30_001443"
     CDS             21089..21946
                     /gene="potB"
                     /locus_tag="ISB30_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAP1989089.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFIFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            21943..22737
                     /gene="potC"
                     /locus_tag="ISB30_001444"
     CDS             21943..22737
                     /gene="potC"
                     /locus_tag="ISB30_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAP1989090.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            22734..23780
                     /gene="potD"
                     /locus_tag="ISB30_001445"
     CDS             22734..23780
                     /gene="potD"
                     /locus_tag="ISB30_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAP1989091.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            23838..24299
                     /locus_tag="ISB30_001446"
     CDS             23838..24299
                     /locus_tag="ISB30_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HAP1989092.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEGSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFISVGLLISMPSSKKTRRKRKSRP"
     gene            24296..25084
                     /locus_tag="ISB30_001447"
     CDS             24296..25084
                     /locus_tag="ISB30_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP1989093.1"
                     /translation="MKKIIILLSLLILLPLTATSKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVAPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(25222..26043)
                     /gene="cobB"
                     /locus_tag="ISB30_001448"
     CDS             complement(25222..26043)
                     /gene="cobB"
                     /locus_tag="ISB30_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP1989094.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(26059..26970)
                     /gene="nagK"
                     /locus_tag="ISB30_001449"
     CDS             complement(26059..26970)
                     /gene="nagK"
                     /locus_tag="ISB30_001449"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAP1989095.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(26999..28243)
                     /gene="lolE"
                     /locus_tag="ISB30_001450"
     CDS             complement(26999..28243)
                     /gene="lolE"
                     /locus_tag="ISB30_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAP1989096.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQDDAWSNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(28243..28944)
                     /gene="lolD"
                     /locus_tag="ISB30_001451"
     CDS             complement(28243..28944)
                     /gene="lolD"
                     /locus_tag="ISB30_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAP1989097.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(28937..30136)
                     /gene="lolC"
                     /locus_tag="ISB30_001452"
     CDS             complement(28937..30136)
                     /gene="lolC"
                     /locus_tag="ISB30_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAP1989098.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            30397..31470
                     /locus_tag="ISB30_001453"
     CDS             30397..31470
                     /locus_tag="ISB30_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAP1989099.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NPLALPLFVLFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKAVPLR
                     RHLLMASLLTVLAVGAWLFNISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSN
                     TIAIYTTHRILVEIFSLSLIPQMNAVRWSPQVELTLLLIYPFVSLLICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPATVSYSR"
     gene            31597..35043
                     /gene="mfd"
                     /locus_tag="ISB30_001454"
     CDS             31597..35043
                     /gene="mfd"
                     /locus_tag="ISB30_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP1989100.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPSMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGIDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFMAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            35186..36148
                     /gene="ldtC"
                     /locus_tag="ISB30_001455"
     CDS             35186..36148
                     /gene="ldtC"
                     /locus_tag="ISB30_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HAP1989101.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPSAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(36231..36488)
                     /gene="bhsA"
                     /locus_tag="ISB30_001456"
     CDS             complement(36231..36488)
                     /gene="bhsA"
                     /locus_tag="ISB30_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAP1989102.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         9146 a         9294 c         9120 g         8976 t
ORIGIN      
        1 tcatttcggg cgttacaact agtcctagat tatattcgct aggtatactt tatttttaag
       61 ctaatacttt agctctattt catcgctcct ttcagcccga acttagcttt gatttctgcg
      121 atcttcgcca gagcctgtgc acgatttaga ggcctaccgc ccatgacagg aagttgtttt
      181 actggttcag gtatagcctc accacggtta attcgcgcgg tcatacaggc cagttcatcg
      241 gcagccttgc gccgtaattc cgcgtcagtc aacgcattgg cccgcatgtt ctgatacagg
      301 ttggtaacca gccagtagtg cgcgtttgat ttccacggat aagactctgc atccggatac
      361 agaccacgct tccggcaata ctcgtaaacc atatcaacca gctcgctgac gtttggcagc
      421 ccggcggtaa cggatgcttc ttcccggcac catgcaacaa actgcccggg tgatggcaga
      481 aatggtctat tctgccgacg ggcaacacgc attccgtcgc tcacctgctc catcgtggtg
      541 atcccgtttt cccggaaagc cagaacccac tggcggcgga tttcgttcag ttcgttctgg
      601 tcacgattag ccaggctcgc cgggaaagtt gccagtaact ggctgaatac accgttgatt
      661 atctgcgcta cctgctgtac ctgcggcttt tcgtcgtact gttccggcat gttgttggcg
      721 atccgacgca tctgctcacg gtcaaagtta atcatctgtg cggcgatgtt tttcatagat
      781 ccaccccgta aatccagtct gtgtttgtca ggtcgagttt tggtttgcta gctgtcacgc
      841 ctgcctgttg cttgttacgg ttgatttcga gttgggtcca cttgtcgcgg agtttggccg
      901 gacttagcac gttaccggac cagaagttgt cctggcatgc ccagcggaac agcacgcaca
      961 tgtcgcggtg gttacgtccg tcacgttcac gcatcaggcg gatatcgtta gcccaccctg
     1021 caaaattcgg ttttctggct gatggcgcga tggtcttcac catgtcaaac atccactctg
     1081 cggcggtcag gtcttctgct gtcccccact tgctgccgct ctgaattgca gcatccggtt
     1141 tcaccacagg aagatcgttt tctggttggt cagaggattc gccagaattc tcggacgaaa
     1201 aaggttttat attgtctttt gttagtttgt cttttgtgtt tacctgattc gggtaaacgc
     1261 ctttacctga tttgggtaaa ctttttttac ctgattcagg taaatttacc tctttcaggt
     1321 aaactttatt tttcttacct gattcgggta atgttgacca ttcactgacc acattattaa
     1381 tgccggtatt ccgcccgctc tgaataaaaa tcccacgctt taccagaaca ctttttgcag
     1441 cagaacactt gtgcggcaat atcccggtta attcggaaag ttgctcgttg ctaacccaat
     1501 ccagtttttt attaaagcca tatgttttgc gcatgacagc cagaaagacc agaagctggt
     1561 gctgtgttaa tccggccagc atcacagctt ccagcaactc atttgcaatg cgcgtataac
     1621 catcatcgag atctgccacg cgcggctcct tttgtgccgc atccggcact ggaaaattga
     1681 atatctcagc agtgtttgcc ataattcctc ccgcaatgag tgtgttacga tttgcacctg
     1741 aaagtcggtt ctgttccagc agaccggctt tcgccatttc tgaacctgtc atatcgcccc
     1801 cagcatggta gtaaccatcg ccatcaatgg accagccaga tctgggtcca cacgaaacat
     1861 cgacacaata ccttcactaa tttccttcag tttctggtgg cgtggtgcgt tgagaatgac
     1921 agcctgtttt gcctcactga gttccttttc catttcagcc aacctagcca tgaagctatc
     1981 ctgctcaacc aggtaaccgc gatattccag cggtagtacc gccagaattg ccggggtcag
     2041 ttcacgcacg ttatttcggt atttttcaga atcgaatttg ttatcgagga agcggaacag
     2101 cttctggcgt gcacggctga catcatcagg gaaatcgatg gtgccgccgc cctgctccag
     2161 atactcattc acaatgagtg tggcaacgac atcctgatta tctacagccg accaggcgcg
     2221 gacggcatca cggatttttt cgtggcctgg cacctgtttt gtttgagaac gatttatcac
     2281 cgcagtcggg ctaaatccgc tagtctgttg gtatgtaagt ggttgcataa ttgactcctt
     2341 tagtttgaat tgactgttaa gttgattgct tattgttaaa gagcgtgaaa tggaaattta
     2401 agctgcgttc ttttcggtgt gtggaaacaa cttcggaaga tccgggcgaa tctggtatgc
     2461 cttcactact ccaccagtag ccgtaacaat gctgccgaca tgttcagggg atacctttgc
     2521 tttgttgtga agccacttat agacggcctg ctgtgaaact tcgcaagcag cgcccagttt
     2581 cttttgtgaa ccaacgatat tgatcgctgt tttgatagct gggttcataa caacctccgt
     2641 ggttaatttg aatcaagatt aaaactatgg ttgtttttag tcaacaacca ttttcgtttg
     2701 atggaataaa accttggttg tacatttgga ctatgaaaac aacactctca gaaagactta
     2761 aagaagccag attagcgcga ggccttacac aaaaggcgct tggggatttg gtcggggtta
     2821 gccaggctgc tattcagaaa atcgaaacag ggaaagctaa tcaaacaact aaaatcgtgg
     2881 agatcgcgaa cgctttgggt gtgcgcgcag aatggttatc ttctggcgtt ggaaatatgt
     2941 cagacagtac agtgcaacca atacaatcaa ctgtcagcca ttccaaatac ttcaagattg
     3001 acgttcttga tatagaagtc agtgctgggc cgggagtcat caaccgtgag tttgtagaag
     3061 ttctacgctc ggttgagtac tcgtttgacg atgctcgtca catgttcgat ggtaggaagg
     3121 cggaaaatat ccgcatcatt aacgtgcgtg gtgacagcat gtcaggaacg atcgaaccag
     3181 gtgatctgct gttcgttgat atcacagtta aatctttcga cggtgatggt atctatgcgt
     3241 ttctgtacga cgacacagcc catgtaaagc gcctgcaaat gatgaaggat aagctgctgg
     3301 tcatctctga taacaaaagc tactcaccgt gggacccgat cgagaaagac gagatgaacc
     3361 gggtgttcat cttcggtaag gttattggga gcatgccgca gacatatagg aagcatgggt
     3421 agtaccaatt aaaaattatc aactgggcat tgtgctcatt cagtaaagaa ctaattccta
     3481 tctttgctct aggtagtaat attaagccac cgcaataata tctttaccta acggcgtaag
     3541 aatcccggtc accgtgccgg gttttctttt gccctcccct catcacacac accgttaaaa
     3601 aaaccaccat aacctcgctt cagttatcgc tatgcgattc aagtcacaaa ataaatccat
     3661 cctaaataca accagttata tctaaaacaa ccaataaaac aacttttgtt gttgacgata
     3721 aaacaactat agttttaaat aagttcatcg caacaacaca acgatacggc aactacctga
     3781 ttcaccgttg cgatgaccgc ttagatccgc agtttgaatt tcagcaggct tcggggagtg
     3841 cgaggggtga aacggacgcg tgaacgtcgg tgtgaccagc tgaaattaac tcaacatttc
     3901 ataccttagt cgcttcaacg aggcggctta gttatgacaa ccggcggcca tccaccgcct
     3961 gaatacgcgc agaagtctct atatgttcag cagcccagct tacgggcagg agtttttatg
     4021 gttcatcaac attacggaac gcagaccgtt aatcgcggtg cggtcatgcc aggaatgctg
     4081 gtcaaacaca aagatggtac ctggactgca tcagctaatt tacgcggacg gctttatctg
     4141 catcgcggca tcgagcgcac ttatacccgt gatttgctcg tggaagtttt tctcgacgga
     4201 cgcggcaacg gcctgaatca ctaatcccct ttcctgttta cctaatcagc ccggcatttc
     4261 gcaggcgata ttttcacagc cattttcagg agttcagcca tgaacgctta ttacattcag
     4321 gatcgtcttg aggctcaaag ctgggcgcgt cactaccagc agatcgcccg tgaagagaaa
     4381 gaggcagaac tggcagacga catggaaaaa ggtcttccac agcacctgtt tgaatcactc
     4441 tgcatcgatc atttgcaacg ctgcggggcc agcaaaaaag ccattacccg tgcgtttgat
     4501 gacgatgttg agtttcagga gcgcatggca gaacacatcc ggtacatggt tgaaaccatt
     4561 gctcaccacc atgttgatat tgattcagag gtataaaacg gatgagtaca gcactcgcaa
     4621 cgctggctgg gaagctggct gaacgtgtcg gcatggattc tgtcgaccca caggaactga
     4681 tcaccactct tcgtcagacg gcatttaaag gtgatgccag cgatgcgcag ttcatcgcat
     4741 tgctgatcgt cgccaaccag tacggcctta atccgtggac gaaagaaatt tacgccttcc
     4801 ctgataagca gaacggcatc gttccggtgg tgggcgttga tggctggtcc cgcatcatca
     4861 atgaaaacca gcagtttgat ggcatggact ttgagcagga caatgaatca tgtacgtgcc
     4921 ggatttaccg caaagaccgc aatcatccga tctgcgttac cgaatggatg gatgaatgcc
     4981 gccgcgaacc attcaaaact cgcgaaggca gagaaatcac ggggccgtgg cagtcgcatc
     5041 ccaaacggat gttacggcat aaagccatga ttcagtgtgc ccgtctggcc ttcggatttg
     5101 ctggtatcta tgacaaggat gaagccgagc gcattgtcga aaatactgca tacactgcgg
     5161 aacgtcagcc ggaacgcgac atcactccgg ttaacgatga aaccatgcag gagattaaca
     5221 ctctgctgat tgccctggac aaaacatggg atgacgactt attgccgctc tgttcccaga
     5281 tatttcgccg cgacattcgc gcatcgtcag aactgacaca ggccgaagca gtgaaagctc
     5341 ttggattcct gaaacagaaa gccactgagc agaaggtggc agcatgacac cggacattat
     5401 cctgcagcgt accgggatcg acgtgagagc tgtcgaacag ggggatgatg catggcacaa
     5461 attacggctc ggcgtcatca ccgcttcaga agttcacaac gtgatagcaa agccccgctc
     5521 aggaaagaag tggcctgaca tgaaaatgtc ctacttccac accctgctgg ctgaggtttg
     5581 caccggtgtg gctccggaag ttaatgctaa ggcgctggcg tggggaaaac agtacgagaa
     5641 cgacgccaga accctgtttg aattcacttc cggcgtgaat gttactgaat ccccgatcat
     5701 ctatcgcgac gaaagtatgc gcaccgcctg ctctcccgat ggtttatgca gtgacggcaa
     5761 tggccttgag ctgaaatgcc cgtttacctc ccgggatttc atgaagttcc ggctcggtgg
     5821 tttcgaggcc ataaagtcgg cttacatggc ccaggtgcag tacagcatgt gggtgacacg
     5881 aaaagatgcc tggtactttg ccaactatga cccacgaatg aagcgtgaag gcctgcatta
     5941 tgtcgtggtt gagcgggatg aaaagtacat ggcgagtttt gacgagatgg tgccggagtt
     6001 catcgaaaaa atggacgaag cactggctga aattggtttt gtatttggag agcaatggcg
     6061 atgacccatc ctcacgataa tatccgggta ggcgcgatca ctttcgtcta ctccgttaca
     6121 aagcgaggct gggtatttca cggtctttct gttatccgaa atccgctgaa agcccagcgg
     6181 ctggctgagg agataaataa taaacggggg gcggtatgca caaagcatct cctgttgagt
     6241 taagaacgag tattgatttg gcacatagcc ttgctcaaat tggagtcagg tttgtgccaa
     6301 taccagcaga aacagacgaa gaatttcata cgttagccac atccctttca caaaagctgg
     6361 aaatgatggt ggcgaaagca gaagcagatg agagagacca ggtatgacaa ccactgaatg
     6421 catttttctg gcagcgggct tcatattctg tgtgcttatg cttgccgaca tggggcttgt
     6481 tcagtgacac ctcagcaaga aaatgccctt cgcagcattg cccgtcaggc taattctgaa
     6541 atcaaaaaag tcagacagca gtttccggat aaaaacgtca atgacatttg ccgtagcgta
     6601 ctgaagaagc accgcgaaac ggtaacgctg aagggattca caccgactca tttaagcctg
     6661 gcaatcggca tgttaaacgg cgtctttaag gaacgatgaa catgaaaagc aaaattatca
     6721 gggagctaca ggctcctttt ttattgttcg cattcaccct caagcgtatt aaccaacagt
     6781 tcagggatta atgaaagatg gcagacatca ttgattcagc atcagaaata gaagaattac
     6841 agcgcaacac agcaataaaa atgcgccgcc tgaactacct ggctgtatct gccactcatt
     6901 gttgtgagtg tggcgatccg atagatgaac gaagacgcct ggccgttcag ggttgtcgga
     6961 cttgcgccag ttgccagcaa gatctggagc ttatccgcaa acaaaggggg attaagtgat
     7021 ggctaacctg caacttgctg ttaaaagtga atacttcgat gccatgattc gcggagagaa
     7081 aacggaagag tatcgcttgt gtaatgacta ctggaataag cgaattatgt tccgggaata
     7141 tgaccgcctg attatcacaa agggatatcc gaagcgcgac gattccagtc gcagaattga
     7201 tgttccgtat gacggatatg aagtgaagac aatcacacat ccgcactttg gcgataaacc
     7261 ggtaaaggtg ttcgctataa aggtgaatat cagcactgaa tatcaatccg cacaacacaa
     7321 ggtcaagaat gttcagagtg attgacccta acacctggta cgtcgaccac cacggcactc
     7381 cctgcaaaat cctgcgttct acccacaaca aagttcacta catccgaaaa ggcagaacat
     7441 gtatcgccag catgttccgc tttaatcatg actttgaacc tgtgaataaa gctgatgcag
     7501 atcggatagc agaagagatc gaaacggcag aacacattaa gaagttacgt gacatgcgca
     7561 ggaaatagaa aaattgataa attcaatact gcatttctca gcattaaatt tatctctatg
     7621 accagtcaag agatgtacct gccatgagct taatatcatg tcagatatat cggtcacaaa
     7681 ctccctcagc agctaagagg aggacaaatg tctcgactaa tcactttaca ggactgggct
     7741 aaagaagaat ttggggactt agcaccaagt gagcgagttc tgaaaaaata cgcgcaaggg
     7801 aaaatgatgg ccccacccgc tataaaagtt ggtcgctact ggatgattga ccgaaattcc
     7861 cgttttgtag gaacgcttgc agaaccgcaa ctcccaataa acgcaaaccc aaaactccaa
     7921 aggataatcg ctgatggctg ctagaccccg atctcacaaa atctctatac ccaatttata
     7981 ttgcaaatta gataagcgaa ccggaaaggt atattggcaa tacaaacatc cactatccgg
     8041 tcgttttcat agcttaggaa ctgatgagaa tgaagcaaaa caagttgcta ctgaagcaaa
     8101 taccattatt gctgaacaac gtaccagaca aatattaagc gtcaatgagc gtctggaaag
     8161 aatgaaaggc aggcgctcag acattacggt gacagaatgg cttgataaat atatttctat
     8221 ccaagaggac aggctgcaac ataatgaact aagacccaac tcctatcggc aaaaaggcaa
     8281 acccattcgt cttttccgtg agcattgtgg aatgcaacac ctcaaggata ttaccgcact
     8341 tgatattgcc gaaataattg atgctgtaaa ggctgaaggt cataacagga tggcgcaagt
     8401 cgtgagaatg gtgttgatcg acgtcttcaa agaagcacaa cacgcaggac atgttccgcc
     8461 aggatttaac ccagcgcagg caacaaaaca accgcgaaat cgagtaaacc gccaaagatt
     8521 gtcactgccc gaatggcagg caatatttga cagcgtaagc agacggcagc cctatttaaa
     8581 atgcggcatg ctacttgctc ttgttactgg acaacgttta ggcgatatct gcaatttgaa
     8641 attctctgat atatgggacg acatgttgca cattactcag gaaaaaaccg gttcaaaact
     8701 tgctattccg cttaacctga aatgcgatgc tctgaatatt acccttcgtg aagttatatc
     8761 tcagtgcagg gatgctgttg ttagtaaata tctggtccat taccgtcaca ctacctctca
     8821 agcaaacaga ggagaccagg tttctgcaaa tactctgaca acggctttta aaaaggcccg
     8881 ggaaaaatgt ggcataaaat gggagcaagg aactgcgccc acatttcatg agcagcgatc
     8941 tctgtcagaa cggttatatc gggaacaggg tctggatacg caaaagttgt taggccataa
     9001 atccagaaaa atgaccgacc gatacaatga tgatcgtggt aaagactgga ttatcgtaga
     9061 tatcaaaaca gcatagaaaa tagccagttt tggggaaggg ttttggggaa agttttgggg
     9121 aagattttac atcatcataa aacaacgggc gtgttatacg cccgtttcaa tatttaacac
     9181 atgtagtgat tacatattct tgatgatcgc gtcaccaaac tctgaacatt tcagcagttt
     9241 agcgccttcc atcagacgtt cgaagtcata ggttacggtc ttggcattga ttgcgccttc
     9301 cataccttta acaatcaggt cagccgcttc agtccaaccc atatggcgca gcatcatctc
     9361 agcggagaga ataatagagc ctgggtttac tttgtcctgt ccggcatatt tcggcgcagt
     9421 accgtgggtg gcttcaaaca gggcgcattc gtcaccgatg tttgcgccag gggcgatacc
     9481 gataccacca acctgcgctg ccagggcgtc agaaatgtag tcaccgttca ggttcataca
     9541 ggcgataaca tcatattcag ccggacgcag caggatctgt tgcaggaatg catcagcaat
     9601 cacgtcttta atgacgatct ctttgccggt attcgggttt ttaactttca gccacgggcc
     9661 gccgtcgatc agttcaccgc caaattcttc acgcgccagt tggtagcccc agtctttaaa
     9721 cgcgccttcg gtgaacttca tgatgttgcc tttgtgcacc agggtcacag agtcacgatc
     9781 gttagcaatt gcgtattcga tcgctgcacg aaccagacgt ttggtgcctt cttccgaaca
     9841 cggcttaata ccgataccgc aatgttccgg gaagcgaatt ttcttcacgc ccatctcttc
     9901 acgcaggaat ttaatcactt tctcagcgtc ggcagagtct gctttccatt cgatacccgc
     9961 ataaatgtct tccgagtttt cacggaagat aaccatatcg gtcagttcag ggtgtttaac
    10021 cgggcttgga gtgccctgat agtaacgtac cggacgcagg cagatgtaga gatccagttc
    10081 ctggcgcagg gcaacgttca gagagcgaat accgccacca accggagtgg tcagcgggcc
    10141 tttaatggca acgcgatatt cacgaatcag atcaagggtt tcagcaggca accagacgtc
    10201 ctgaccataa acctgtgtgg atttttcacc ggtgtaaatt tccatccagg agattttacg
    10261 ctcgccttta taggctttct cgactgcagc gtcgaccact ttcagcatgg ctggggttac
    10321 atctacaccg attccatcac cttcaatgta agggataatc ggattttcag gaacgttgag
    10381 tttgccgttt tgcagggtga tcttcttgcc ttgtgccgga acaactactt tactttccat
    10441 tcacctctcc ttcgagcgct actggtttgc tcgtctgcca ccacgttgca tatgcgtttg
    10501 cgtcctgcga tacggatgct ttagagcaat tttttgttaa tgatttgtaa ttagcttgtc
    10561 aatactaccc tattgtttgc cgtcatgaaa gatgcgcgtt attaggctat aatgcggcaa
    10621 ttcataatct ctgaaaatac catgcaaaaa acttctttta gaaatcacca ggttaagcga
    10681 ttcagctcgc aacgttctac caggcgtaaa cctgacaacc agcccacgcg tgtgatcctg
    10741 ttcaataaac cctacgatgt tcttccgcag ttcaccgatg aagccggacg caaaacatta
    10801 aaagaattca tcccggttca gggggtgtat gcggcaggtc gtctcgaccg cgacagcgaa
    10861 gggttactgg tgctgaccaa taacggcgcg ttgcaggcgc gtttaaccca gccgggtaaa
    10921 cgcaccggaa aaatctatta tgtgcaggtg gaaggtattc ccacacaaga cgcacttgaa
    10981 gccttgcgca atggcgtaac cttaaatgat ggccctaccc tgcccgcagg cgcggagctg
    11041 gttgaagaac ccgcgtggtt atggccgcgg aatccaccaa ttcgtgagcg caaaagtatt
    11101 cccaccagct ggctgaagat caccttatat gaaggacgta atcgccaggt gcgccgcatg
    11161 accgcccatg ttggtttccc cacgctgcga ctgattcgct atgcgatggg ggattactct
    11221 ttggataatc ttgccaatgg cgaatggcga gacgcgacag attaaggatg caaaatgttt
    11281 aaaccgcacg ttaccgttgc ttgcgtggtg cacgcagaag gcaaattttt agtcgttgaa
    11341 gagacgatta atggtaaagc gttatggaac caacctgccg ggcacctgga agccgatgaa
    11401 accttagtgg aagccgccgc ccgtgagctg tgggaagaaa ccggcattag cgcgcagccg
    11461 caacacttta ttcgtatgca tcagtggatt gcgcccgata aaacgccgtt tttgcgtttc
    11521 ctctttgcca ttgagcttga gcaaatatgc ccgacgcagc ctcatgacag cgatatcgac
    11581 tgctgccgtt gggtcagcgc cgaagaaatt ttacaggcgt caaatcttcg ttcgccgctg
    11641 gtggcggaaa gtattcgttg ttatcaaagc gggcaacgct atccgctgga gatgattggc
    11701 gattttaact ggccttttac aaagggtgtc atctaaaagg ctgcgtgata gaatacgccg
    11761 ccttgaagtt caatgtcgtg agtgatccaa tgtctgaaac cgcaaaaaaa gtaatcgtcg
    11821 gcatgtccgg cggtgtcgat tcctccgttt ctgcctggct gttacaacaa cagggatatc
    11881 aggtcgaagg cctgtttatg aagaactggg aagaagacga cggtgaggaa tattgcacag
    11941 cggcagcgga tctggctgat gcccaggctg tctgcgacaa gctcggcatt gaactgcaca
    12001 ccgttaactt tgctgccgag tactgggaca acgtcttcga actgttcctt gccgaatata
    12061 aagccggtcg cacgccgaat ccggatattc tgtgcaacaa agagatcaaa tttaaagctt
    12121 tcctcgaatt tgccgccgaa gatttaggtg ccgattatat cgctaccggt cattacgtac
    12181 gtcgggcaga tgtcgatggc aagagccgcc tgctgcgtgg tctggacagc aataaagacc
    12241 agagctactt cctttatacg ctcagccatg agcagattgc gcaaagcctg ttcccggtcg
    12301 gcgaactgga aaaaccgcag gtgcgtaaga ttgctgaaga tcttggtctg gtcaccgcga
    12361 agaaaaaaga ctctaccggc atttgcttca ttggcgaacg taaattccgc gagttcctgg
    12421 gccgttatct cccggcgcaa ccgggcaaaa tcattaccgt cgatggcgat gaaattggtg
    12481 agcaccaggg gctgatgtat cacaccctcg gtcagcgtaa aggtctgggt atcggtggca
    12541 ccaaagacgg caccgaagaa ccgtggtatg tggtcgataa agacgtcgaa aacaacattc
    12601 tgattgttgc gcaaggccat gaacacccgc gtctgatgtc tgtcggtctg attgcccagc
    12661 agctgcactg ggtcgatcgc gaaccgttca ccggtactat gcgttgcaca gtaaaaaccc
    12721 gctatcgcca gaccgacatc ccgtgcaccg tcaaggcgct ggacgatgat cgcattgaag
    12781 tgattttcga tgagccggtt gccgccgtga cgccgggcca gtctgccgtt ttctataacg
    12841 gtgaagtgtg cctcggtggc ggtattattg agcagcgtct gccgctgccg gtctgattat
    12901 tatctttact taaaacaagg aagcagtgaa cgtggcaaag aattactatg acatcaccct
    12961 cgccctggcc ggtatttgtc agtcggcacg cctggtgcaa caactcgctc accaggggca
    13021 ttgtgatggc gatgcgctac acgtctcact caacagtatt atcgatatga accccagctc
    13081 aacgctggcg gtttttggcg gcagcgaagc caacctgcgc gtcgggctgg aaaccttgct
    13141 cggcgtactc aatgccagca gtcgccaagg cttaaacgcc gaattaaccc gctacacact
    13201 cagtctgatg gtgcttgagc gcaaactctc ctcagcgaaa ggtgcgctcg acactctggg
    13261 caaccggatc aacggcctgc aacgccagct cgaacacttc gatttacagt ccgaaacgct
    13321 gatgagcgcg atggctgcca tctatgttga tgttatcagc ccgcttggcc cgcgcattca
    13381 ggtcacgggt tcccctgctg tactgcaaag cccacaggtg caggcgaaag ttcgcgcaac
    13441 cctgctggca ggcattcgcg ccgccgtgct ctggcaccag gtcggcggcg gtcgtctgca
    13501 actgatgttt tctcgtaatc gcctgaccac tcaggcaaaa caaattcttg ctcatttaac
    13561 cccggagttg tgatctatgg aattatcctc actgaccgcc gtttcccctg tcgatggacg
    13621 ctacggcgat aaagtcagcg cgctgcgcgg gattttcagc gaatacggtt tgctgaaatt
    13681 ccgtgtacaa gttgaagtac gttggctgca aaaactggcc gcgcacgcag cgatcaagga
    13741 agttcctgct tttgctgccg acgcaatcgg ttaccttgat gcaattgtcg ccagtttcag
    13801 cgaagaagat gcagcacgca tcaaaaccat cgagcgtacc actaaccacg acgttaaagc
    13861 ggttgagtat ttcctgaaag aaaaagtggc ggatatcccg gaactgcacg cggtttctga
    13921 attcatccat tttgcctgta cttcggaaga tatcaataac ctctcccacg cattaatgct
    13981 gaaaaccgcg cgtgatgaag tgatcctgcc gtactggcgt caactgattg atggcattaa
    14041 agatctcgcc gttcagtatc gcgatatccc gctgctgtcc cgtacccacg gtcagccagc
    14101 cacgccgtca accattggta aagagatggc taacgtcgcc taccgtatgg agcgccagta
    14161 ccgccagctt aaccaggtgg agatcctcgg caaaatcaac ggcgctgtcg gtaactacaa
    14221 cgcccacatc gccgcttacc cggaagttga ctggcatcag ttcagcgaag agttcgtcac
    14281 ctcgctgggt attcagtgga acccgtacac cacccagatc gaaccgcacg actacattgc
    14341 cgaactgttt gattgcgttg cgcgcttcaa caccattctg atcgactttg accgtgacgt
    14401 ctggggttat atcgccctta accacttcaa acagaaaacc attgctggtg agattggttc
    14461 ttccaccatg ccgcataaag ttaacccgat cgacttcgaa aactccgaag ggaacctggg
    14521 cctttccaac gcggtattgc agcacctggc aagcaaactg ccagtttccc gctggcagcg
    14581 tgacctgacc gactccaccg tgctgcgtaa cctcggcgtg ggtatcggtt atgcgctgat
    14641 tgcgtatcaa tccaccctga aaggcgtgag caaactggaa gtgaaccatg accatctgct
    14701 ggaggagctg gatcacaact gggaagtgct ggctgagcca atccagacag ttatgcgtcg
    14761 ctatggcatc gaaaaaccgt acgagaagct gaaagagctg actcgcggta agcgcgttga
    14821 cgccgaaggc atgaagcaat ttatcgacgg tctcgcgctg ccagaagaag agaaagcccg
    14881 cctgaaagcg atgacgccgg ctaactatat tggtcgcgcc atcaccatgg ttgatgagct
    14941 gaaataagcc tcaaatcagt gccggatggc gatgctgtcc ggcctgctta ttaagattat
    15001 ccgcttttta ttttttcact ttacctcccc ttcccgctgg tttatttaat gtttaccccc
    15061 ataaccacat aatcgcgtta cactatttta ataattaaga cagggagaaa taaaaatgcg
    15121 cgtactggtt gttgaagaca atgcgttgtt acgtcaccat cttaaagttc agatacagga
    15181 tgctggtcat caggtcgatg acgcagaaga tgccaaagaa gccgattatt atctcaatga
    15241 acatttaccg gatattgcga ttgtcgatct cggattgcct gacgaggatg gtctgtcact
    15301 gattcgccgc tggcgtagca atgatgtttc actgccgatt ctggtattaa ccgcccgtga
    15361 gagctggcag gacaaagtcg aagtattaag tgccggtgct gatgattacg tgactaaacc
    15421 gtttcatatt gaagaggtga tggcgcgaat gcaggcatta atgcggcgta atagcggtct
    15481 ggcttcacag gtcatttcgc taccaccgtt tcaggttgat ctctcccgcc gtgaattatc
    15541 tattaatgac gaagtgatca aactgaccgc gttcgaatac accattatgg aaacgttgat
    15601 acgcaataat ggcaaagtgg tcagcaaaga ttcgttaatg ctacaactct acccggatgc
    15661 ggagctgcgg gaaagccata ccattgatgt actgatggga cgtctgcgca aaaaaattca
    15721 ggcacaatat ccgcaagaag tgattaccac cgttcgcggc cagggctatc tgttcgaatt
    15781 gcgctgatga aaaaattact gcgtcttttt ttcccgctct cgctgcgggt acgttttctg
    15841 ttagcaacgg cagcagtagt attggtgctt tcgcttgcct acggaatggt tgcgctgatc
    15901 ggttatagcg tcagtttcga taaaaccacg tttcggctgt tacgtggcga gagcaatctg
    15961 ttctataccc ttgcgaagtg ggaaaacaat aagttgcatg tcgagttacc cgaaaatatc
    16021 gacaagcaaa gccccacgat gacactaatt tatgatgaga acgggcagct tttatgggcg
    16081 caacgtgacg tgccctggct gatgaagatg atccagcccg actggctaaa atcgaacggt
    16141 tttcatgaaa ttgaagcgga tgttaacgat accagcctct tgctgagtgg agatcattcg
    16201 atacagcaac agttgcagga agtgcgggaa gatgatgacg acgcggagat gacccactcg
    16261 gtggcggtaa acgtctaccc ggcaacatcg cggatgccaa agttaaccat tgtggtggtg
    16321 gataccattc cggtggagct aaaaagttcc tatatggtct ggagctggtt tatctatgtg
    16381 ctctcagcca atctgctgtt agtgatcccg ctgctgtggg tcgccgcctg gtggagttta
    16441 cgccccatcg aagccctggc aaaagaagtc cgcgaactgg aagaacataa ccgcgaattg
    16501 ctcaatccag ccacaacgcg ggaactgact agtctggtac gaaacctgaa ccgactgtta
    16561 aaaagtgaac gcgaacgtta cgacaaatat cgtacgacgc tcaccgacct gacccatagt
    16621 ctgaaaacgc cactggcggt gctgcaaagt acgctgcgtt ctctgcgtag tgagaagatg
    16681 agcgtcagtg atgctgagcc ggtaatgctg gagcaaatca gccgcatttc acagcaaatt
    16741 ggctactacc tgcatcgtgc cagtatgcgc ggcgggacat tgcttagccg cgagctgcat
    16801 ccggtcgccc cactgctgga caatctcacc tcggcgctga acaaagtgta tcaacgcaaa
    16861 ggggtcaata tctctctcga tatttcgcca gagatcagct ttgtcggtga gcagaacgat
    16921 tttgtcgagg tgatgggcaa cgtgctggat aatgcctgta aatattgcct cgagtttgtc
    16981 gaaatatctg caaggcaaac agacgagcat ctctatattg tggtcgagga tgatggcccc
    17041 ggtattccat taagcaagcg agaggtcatt ttcgaccgtg gtcaacgggt tgatacttta
    17101 cgccctgggc aaggtgtagg gctggcggta gcccgcgaaa tcaccgagca atatgagggt
    17161 aaaatcgtcg ccggagagag catgctgggc ggtgcgcgga tggaggtgat ttttggtcgc
    17221 cagcattctg caccgaaaga tgaataaata tgtccatact tcacgcatta cgttaagcat
    17281 ccgttataat cggttgcaga taccagcctg tggatgctta acatggaata ccaactcact
    17341 cttaactggc ccgattttct tgaacgtcac tggcagaaac gtccggtggt gttaaaacgc
    17401 ggctttaata attttattga cccgatctct ccagacgagt tggcgggttt ggcgatggaa
    17461 agcgaagtcg acagtcgact ggtcagtcat caggatggca aatggcaggt cagccacggt
    17521 ccattcgaaa gctacgatca tctcggtgaa actaactggt cattgttagt gcaagcagtg
    17581 aaccactggc atgagccgac cgccgcgcta atgcgaccgt tccgtgaact accggactgg
    17641 cgtattgatg atctgatgat ctctttttct gtaccgggcg gcggcgtcgg cccgcatctc
    17701 gatcagtacg acgtgtttat cattcagggt accggtcgtc gtcgctggcg agtgggcgaa
    17761 aagctgcaaa tgaaacagca ctgccctcac ccggatctgt tacaggtcga tccgttcgaa
    17821 gccatcatcg atgaagagct ggagcctggc gatattcttt atattccgcc aggattcccg
    17881 catgaaggct acgcgctgga aaatgcgatg aactattccg tgggttttcg cgcgccaaat
    17941 acgcgggaac tgattagcgg atttgccgat tatgtgctgc aacgtgaact gggcggcaac
    18001 tactacagcg atcctgatgt tccacctcgc gctcatcctg cggacgttct gccgcaagag
    18061 atggataaac tgcgtgagat gatgctcgaa ttgatcaacc agccagaaca ttttaagcaa
    18121 tggtttggcg agtttatatc ccagtcacgt catgaactgg atatcgcgcc gccagagccg
    18181 ccttatcagc cggatgaaat ctacgatgcg ctgaaacaag gtgatgtgct ggtgcgcctg
    18241 gggggtctgc gcgtattgcg cattggcgac gacgtgtatg ccaatggtga gaagatcgat
    18301 tccccgcacc gtccggcact ggatgcgctc gccagcaaca ttgcgctgac cgcggagaat
    18361 tttggcgatg cgctggaaga tccgtcattc ctcgcgatgc tcgcggcgct ggtcaatagc
    18421 ggatactggt tcttcgaagg gtaagtttgg atttaagccg gatgcggcat cacatgccgc
    18481 atcccttttc gcttacttcc gttgcgccgt caactcggca atacggacga tcacctgcac
    18541 cgctttttcc ataccttcca gagtcacaaa ctcatgctta ccatgatagt tgtaaccgcc
    18601 agtgaacagg ttcgggcacg gtaatcccat aaacgacaac tgcgcgccgt cggtaccgcc
    18661 gcggatcggt ttcagttccg gttcaatatc gcagtcacgc atcgcctgct gggcgatatc
    18721 gagaatatgc ggatgctcaa ccactttctc gcgcatattg tagtaactgt cttcaatcac
    18781 cagttcaatg tagcaatcag gatgtaaccc tttgcccact tttttggcga tctccatcat
    18841 tttacgttta cgcgcttcaa actgtttacg gtcgaaatca cggatgatgt agtgcatatc
    18901 ggcccgttca acggtgcctt tcatgctcgc caggtgatag aaaccttcat agccttctgt
    18961 catttccggg ctttcatccg ccggaacttc cgcatgaata cgtgccgcca gcgacagcgc
    19021 atttaccatc actcctttcg ccgtgcccgg gtgaacattg ttaccgacaa ttttgatatt
    19081 gaccgacgcg gcgttgaagt tttcaaactc cagttcacct acgccaccac catcaacggt
    19141 gtaagcccag cgggcatcga aggcgtcaac atcaaaatgt ttcgcccctt tgcccacttc
    19201 ttcatccggg gtaaaggcga cgcgaatatc accatgcgga atgttttttt gttgcaatac
    19261 cgccagcgcg gtcatgattt ctgcaatacc tgctttgtca tcggcaccta acaaggtttt
    19321 accatcggtg gtaatcagcg tctgacccag tagctgatgc agcaccggga acataaccgg
    19381 tgataaaact tcatcgccga tacccagcgc aatatcgcca ccgcgatagt tttcaacaat
    19441 ttgcggattc acatttttgc cgctgcaatc cggtgaggta tccacatgag aaataaagcc
    19501 aatcgccggg atatcgccag ggacgttagc cggtaacgtc gccatcaaag tgcccttctc
    19561 acttaaggtc acattgataa gccccatctc ttcgagctgc tctttcagca gatgcaataa
    19621 cttccattgg ccttccgtgc tgggaacctg tctcacccct gcctttgatt gggtatcaag
    19681 agacacgtag ttcaaaaatc gctcaagtag tttatccatg tagtcaccct cactttttgt
    19741 gacaacatta ttaagaagca agaaaagaca aatattgcgt caggtcactt ttagccctgc
    19801 aagcgggaat atttatcagc attaccttaa tgaatataaa gctaagccag taattcacaa
    19861 caaagattgg cgattcaagc ggtttgccgt agaattaccg ccctcattaa gagtcaccaa
    19921 ggtggttaac cacaaacccc gcatcggtaa gccatccgtt gcgtttacat gggacagagt
    19981 aaaaaattga ataaacaacc gagttcgctt tcaccgctgg tgcaattggc gggaattcgc
    20041 aaatgctttg atggtaaaga ggtcattccc cagctggatc tgactatcaa caatggcgag
    20101 ttcctcacgc tgcttggccc ttctggctgc ggtaaaacaa ccgttcttcg cctgattgca
    20161 ggtctggaaa ctgttgattc cggacgcatc atgctggata acgaggacat cacccacgtt
    20221 ccggcggaaa accgctatgt gaacactgtt ttccaaagct acgcactttt cccccacatg
    20281 accgtgttcg aaaatgtggc ctttgggttg cgcatgcaaa aaacccccgc agctgaaatt
    20341 acgccccgcg tgatggaagc cctgcggatg gtgcagctgg aaaccttcgc tcaacgcaaa
    20401 ccgcatcagc tctctggtgg tcaacagcaa cgcgtcgcca ttgctcgcgc ggtggttaac
    20461 aagcctcgtc tgttgttgct ggatgagtcg ctctcagcgc tggattacaa actgcgtaag
    20521 caaatgcaga acgagctgaa agcgttacag cgtaagcttg gcattacatt cgtgttcgta
    20581 actcacgacc aggaagaagc acttaccatg tcggacagga tcgtggtgat gcgcgatggt
    20641 cgcattgagc aagacggcac gccgcgtgaa atctacgaag agccgaaaaa cctgtttgtt
    20701 gccgggttta ttggcgaaat caatatgttt aacgccactg tcatcgaacg tctcgacgag
    20761 cagcgcgtac gcgccaacgt tgaaggccgc gaatgtaata tctacgttaa cttcgccgtt
    20821 gaaccggggc aaaaactgca tgttctgctg cgcccggaag acttacgtgt tgaagagatt
    20881 aacgacgaca accacgccga agggctgatt ggttacgttc gcgagcgtaa ctacaaaggc
    20941 atgacgctgg agtcggttgt tgagctggaa aatggcaaga tggtgatggt cagcgaattc
    21001 ttcaatgaag acgatcctga ctttgaccac tctctcgacc aaaaaatggc cattaattgg
    21061 gtagaaagct gggaggtcgt actggctgat gaagaacaca agtaagttcc agaatgtagt
    21121 gattgtcact attgtcggtt ggcttgtgtt gtttatcttt ctgcccaacc tgatgatcat
    21181 tggcaccagc tttttgaccc gcgacgacgc cagtttcgtc aaaatggtct ttacgctgga
    21241 taactacacg cgtctgctcg atccgctcta ttttgaagtg ctattgcact cgctgaatat
    21301 ggcgctgatc gccaccctcg cctgcctggt gctgggctat ccttttgcct ggtttctggc
    21361 gaagctgcca cacaaggtgc gtccgctgct gctgtttctg ctgattgttc cgttctggac
    21421 caactcattg attcgtattt acgggctgaa aattttcctc agcaccaaag gctatctcaa
    21481 cgagtttttg ctctggctgg gcgttatcga tacaccaatt cgcatcatgt tcacgcctag
    21541 tgcggtgatt atcggtctgg tttacattct gctgccgttt atggtgatgc cgctgtactc
    21601 cagtatcgaa aaactggata agccgttgct tgaggcggcg cgcgatctcg gtgccagcaa
    21661 gttgcagacc tttattcgca tcattatccc actgacgatg ccgggaatta ttgccggatg
    21721 tctgctggtg atgctgccag cgatgggcct gttctatgtg tccgacctga tgggcggtgc
    21781 gaaaaacctg ctgatcggta acgtcatcaa ggtacagttc ctcaatattc gtgactggcc
    21841 gtttggtgca gccaccagca ttacgctgac tatcgtaatg ggcctgatgt tgctggttta
    21901 ctggcgcgct tctcgtttgc tgaataagaa ggtggaactc gaatgatcgg tcgactgctt
    21961 cgcggcggtt ttatgaccgc tatctacgcg tacctgtata tcccaatcat tattttgatt
    22021 gtgaactcct ttaacagctc gcgctttggc atcaactggc agggttttac caccaaatgg
    22081 tatagcctgc tgatgaacaa cgacagcctg ttacaggcag cgcagcattc acttacaatg
    22141 gcggtgtttt cggcgacgtt tgctacgctt atcggttcac tgacggcagt tgcgctgtac
    22201 cgttatcgct ttcgtggtaa gccgttcgtt agcggaatgc tgtttgtggt gatgatgtca
    22261 ccagatatcg taatggcgat ttcgctgctg gtgctgttta tgctgctggg tattcagctt
    22321 ggcttctggt cgctgctgtt ctcgcatatc accttctgcc tgccatttgt ggtagtgaca
    22381 gtgtattcgc gcctgaaagg ttttgacgtg cgaatgctgg aagcggcgaa agatctcggt
    22441 gccagcgaat ttaccattct gcgaaaaatc attctgccac tggcaatgcc agcggtagcg
    22501 gcgggctggg tgttaagctt taccctgtcg atggacgacg tggtggtttc ttcgtttgtc
    22561 accgggccga gttatgaaat tctgccgtta aaaatttatt cgatggtcaa agtcggcgta
    22621 tcgccggaag ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt gatggtaatt
    22681 gccagccagc ttattgctcg tgataaaacg aaaggtaaca caggggacgt taaatgaaaa
    22741 aatggtcacg ccacctgctc gcggcgggtg ctctggcact gggcatgagc gccgctcacg
    22801 ccgatgacaa caacacgctg tatttctaca actggaccga gtacgtgccg ccaggactgc
    22861 ttgaacagtt caccaaagaa accggtatta aggttatcta ttcgacttac gagtcgaacg
    22921 aaaccatgta cgcgaagctg aaaacgtaca aagacggtgc ctatgacctg gtggttcctt
    22981 caacctatta cgtcgataaa atgcgtaaag aagggatgat ccagaagatc gacaagtcga
    23041 agttaagcaa tttcagcaac ctcgatccag acatgctcaa caagccgttt gacccgaata
    23101 acgactattc cattccgtat atctggggtg cgacggcgat tggcgttaac ggtgatgcgg
    23161 tggatccgaa atctgtcacc agctgggccg atctgtggaa gcctgagtac aaaggcagcc
    23221 tgctgctgac cgacgatgcc cgtgaagtgt tccagatggc gctgcgtaag ctgggctact
    23281 ccggtaacac caccgatccg aaagagattg aagctgcata caacgagctg aaaaagctga
    23341 tgccaaacgt cgcggcgttt aactccgata acccggcgaa cccgtatatg gaaggcgaag
    23401 ttaacctcgg catgatctgg aacggttctg ctttcgttgc acgccaggcg ggtacgccaa
    23461 ttgatgtggt gtggccgaaa gaaggcggta ttttctggat ggacagcctg gcgatcccgg
    23521 caaatgccaa aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg cgcccggatg
    23581 tggcaaaaca ggttgctgaa actatcggtt atccaacgcc aaaccttgcg gcgcgtaagc
    23641 tgttaagtcc ggaagtggcg aacgataaaa cgctctaccc ggatgctgaa accattaaaa
    23701 atggtgaatg gcagaatgac gttggcgcag ccagcagcat ttatgaagag tattatcaga
    23761 agctgaaagc aggacgttaa ttcagcaagc gggcggtgat attaccgccc gttttttatt
    23821 tagcaaggat gtggctaatg agccaggaca gtagagtttt ctttcgtata ttcttcggta
    23881 tcggtctggt actgatcctc atttctgttg ttatctttta taaccagttc acctatagca
    23941 aagacgcgat tcatactgaa ggcgtcattg ttgataccgt ctggcactcc agtcattctc
    24001 atcgcacggg taaaaatggt tcgtggtatc ccgtcgttgc ctttcgtccg acgccagatt
    24061 acacgctaat tttcaattcg agtattggca gcgattttta tgaaggcagc gaaggcgata
    24121 aagttaatgt ttattattct ccaggacatc ctgaaaaggc tgagataaat aacccgtggg
    24181 ttaatttttt taaatggggg tttatcggca ttatgggcgt tatttttatc tccgtcggtt
    24241 tattaatcag tatgccctcc tctaaaaaga cacgcaggaa acggaagtcc cgcccatgaa
    24301 aaaaatcata atattgctca gtttactgat actcctgcct ctgacggcaa ccagtaagcc
    24361 cttaattcca ataatgaaga cactttttac ggatgttacc ggtactgtac cagatgctga
    24421 agagatcgcg cataaagccg agctttttcg ccaacaaacc ggagttgcac cgtttattgt
    24481 ggttttaccc gacattaata atgaagccag tctcaggcaa aatggcaaag cgatgctggc
    24541 gcatgcggca tcttcaatga gtaatgtaaa aggaagtgtt ctgttactct ttactacccg
    24601 cgagccacgg ttaattatga taaccaacgg ccaggttgaa agcggtctgg acgataaaca
    24661 tctcggcctt ctggtagaaa atcacacgct ggcttattta catgcggacc tctggtatca
    24721 gggtatcaat aatgcattgg ctgttttaca agcacagata ttaaaacaac cgacgccacc
    24781 actgacatat tatccgcatc ccggacaaca gcatgaaaat gatccacccg gcagtaccac
    24841 gacattaggc ctttttgcct gggcagttgc attcatcgtc tttgctgcat tttttaacta
    24901 cacaacgcgc ctttattatg cgttaaaatt tgcagtggcg atggctgttg ccaatatggg
    24961 ttatcaggct ttatgtttgt atatcgataa cagctttgcg attaccagaa tatcgccact
    25021 ctgggcaggg ctgattggtg tctgtacgtt tattgccgcg ttactatgga cgagtaaacg
    25081 gtaaaagagg cagtaaagtt cgcgtgtatt atgccggatg cggcgcgaac gccttatccg
    25141 gcccacaaaa cccgcaaatt caattaattg cgccccttca caggcctgac aagcgtagcg
    25201 catcaggcaa tttttcccgc tttataatcc cttcagcaac ttttcaacaa actctggcac
    25261 cacctggctt gccggaccgt aatatttctc ggcaaattca ttaccaacct gactcggttc
    25321 aagattcagt tccacggtgt gcgcgccatg cagtttcgct tcgtgaacaa acccagccgc
    25381 cggataaaca tgccccgaag taccaatggc aatgaaaata tcggccatcg acaacgccat
    25441 ataaatttca tccatgccga gtggcatttc gccaaaccac actacgtgcg ggcgcaaggg
    25501 tgccgggaac tggcaacaat ggcatttatc ttctggcgta acgtctccgg tccagtcgag
    25561 gacctggcca ctttgcgaac aacgtacttt cagcaattcc ccatgcatat gaatcacatt
    25621 ggtattgcct gcgcgttcat gcaggttatc gatattttgc gtcaccagca aaaagcgatc
    25681 gccgagagca tcttgcagtt tcgccagcgc aagatgtgca gcgttaggct gaatttctgg
    25741 ctgctgcaac tgtcgacgac gggcgttata aaacgtttgc accagttcag gatcgcgatc
    25801 aaaaccttcc ggcgttgcca catcttcaac ccgatgttct tcccacaggc catctgcggc
    25861 gcgaaaggta cgaatacctg attccgcaga aatccctgcc cctgtcagta cgagtactct
    25921 tggtttttcc attgcttccg gcaccacttt atctctgaaa aaaatacgct gacgcaaacg
    25981 ctcgcgcagg cggcgtttat ttttacgaaa acgacttaac cgatgacccc gacgcgacag
    26041 catagcaacc tctgttgatt aatcggttag atgtaggaag gccgcaccac gcattcctcc
    26101 cgcatcaccg tggcgcgcgc gttcaatgcg cggaacacga gctacaggta agagatgacg
    26161 aggcaacctt tccgccagtt gcgttgtgat tgccgggaaa ttcgataagc caccaccaat
    26221 gacgaccagg tcagggtcaa caatggtcag gatatttccc agacaaaccg ctaataaatc
    26281 cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct tgatcataaa gcgcaatgat
    26341 ctcaggagcc tgcaacggtt gatgataata gtgttgatac agccacgcaa aaccgcgacc
    26401 agacagataa ttttcaatgc agccatgctg accacagccg cagcggcgta acgggaaatc
    26461 cagccccatc atggttaacg catcaaccgg cagacgcata tggccaaact cgccggtaat
    26521 atagctttta ccggtaattg gtttaccgtt gaaaatcagc ccgccgccaa cgccggtgcc
    26581 gagaatcaac cccatcacca gtggatattg agtaaattca tcatcccatg cttcagaaag
    26641 ggcaaaacag ttggcatcgt tatcaaggcg tacatcgcga tcaagacgtg cgctcaggtc
    26701 ggcacgcagc ggtttaccgc tggcagcagg gacattggcg gcatacagcg taccatcttc
    26761 tgtttccggc atacccggaa taccaatgcc gacagaacct ttacagccaa aacgttggtc
    26821 agcttcggct accagctcgc acactgcatc taaaaatgcg tcatagctgt cacgcggtgt
    26881 cggcacccgc ttttcccact gcaactgccg accgctatca aacacgccta gcgcaatttt
    26941 tgttccacca atatcaaacc cgtaatacat tgccgctcct tgttttaatg tactgccttt
    27001 actggccgct aaggactcgc gcagggtcaa tattactggc gcgccgcgcc ggataccaac
    27061 ttgccaaaag actcagcaac aatgctgtga ccagtacgta gaagacgtcc agccagtgca
    27121 attccgatgg caggaagtca ataaaataga tatcgctgga gaggaactga tggccgatca
    27181 gcttttcaat ccactcaata atcggggtaa gttgcagtga aacaactacg ccgataatca
    27241 caccacacag gctgccgaac agccctgcca gcaatccata ccagacaaag atggcgcgga
    27301 ttaaaccatc tttcgcaccc aacgttctta atactgcgat atcgccactc ttgtctttca
    27361 ccgccatcac taaggtggag acgatgttga aacaggccac accaatcacc agtaccatcg
    27421 ccagatacat aatggcgcgg atcatctgaa tatcgcgata catatagccg taagtaccaa
    27481 tccagctttt aatataaaca tagctgttgg tcacttcacc cgcatcgcgt accagcttat
    27541 tggcgttgaa aacatccgtc attttaaggg caatacctga cacgctggaa cccatatcaa
    27601 gatattgctg agcatcggcc agcgggatca tggcaaaact gtgatcgagt tgaccactca
    27661 actgcaaaat accggcaacg tgcaaacgca cacgttttgg ctgcatcagt ttatgctcgg
    27721 gattcgaatt tgggatcata atcgacaccc aatccccctg cttcactttc agcgcatccg
    27781 ccacgccttt gccgatgata atttgctgtt cgcctgcttt aaaattgctc cacgcatcgt
    27841 cctggacaaa cgagggtaat gcgctcaaac gctgttcctg ttgcgggtta acgcccttga
    27901 cctggattgc gcgcagattc gcgccacttt ccaccagccc ggtgaaattg atatacggcg
    27961 cagcggcggc aatgcccggc actttctgca cgttatccag tgcttcctgc cagttggtcc
    28021 acggctgatc taccgcctca atttcaccgt gcggtactac cgccagaatg cggttattca
    28081 gttcgcgttc aaagccgttc atcgcgctta agccgacgat caataccgcc acgccgaggg
    28141 caatgccaat ggtagaaatg acggagatca acgacaccat gccgccgcgt cgccgtccgc
    28201 ggctaaaacg caggccaatc aataacgata aaggcatcgc cattactccg cccccatcag
    28261 gctcagttcc gccgtcagac gtccatcacg catttctagt tggcggctca tacgtttcgc
    28321 cagttgcagg tcgtgagtaa ccaccaggaa ggcggtgccc tgcaagcgat tcaattcccc
    28381 aagcaactgg aagatgctgt cggcgttacg tgcatcgagg ttaccagtag gttcatccgc
    28441 cagtaccagc cgcgggttat tgaccagcgc acgggcaatc gccacacgct ggcgttcgcc
    28501 gccagaaagt tcagatgggc ggtgattcgc acgatgctcc agccccaccg cttttaacat
    28561 ctccagcgca cggctgttga tttcagcggg ctttttcttg ccaatcaaca gcggcatagc
    28621 cacgttttcc agggcggtaa aatccggcag caggtggtga aactgataaa taaatcccag
    28681 cttctggttg cgcagttctg ctttcgccgc cgaagacagt ttgctcatcg gttgaccgtt
    28741 aaagatcaca tcgccggagg ttggcgtatc cagcccgcct aacaggtgca gcaaggtgct
    28801 tttaccggaa ccagagctgc cgacgatcgc catcatttca ccttcgccca cgctgaaact
    28861 gacattgtgc agtacatcgg tttgcacact gccttcctga tagcgtttgc acaggttgtc
    28921 gcattgcaac aggatcttat tcataacgta aagcctcagc gggttgagtg gcggcagcgc
    28981 gccaggaagg gtaaagcgta gacagcagcg cgatagccat cgccaccagc gcaataacaa
    29041 tgacctgtaa aggctcgata gcgaccggca gcgccgcgcc atcaagcagg acgccgatta
    29101 tcggcatcag attattcaac tggctggcaa gcagtgcgcc aagcgccgct ccgaggatcg
    29161 caccgataat cccggcgctg gccccttgca ccataaagac catcatgatt tgccgcggag
    29221 tcagcccctg agtctgaaga atggccactt cgccctgctt ctccatcacc atcagtccca
    29281 gcgaggtaat aatgttaaac gccgcaacgg cgacaatcag gctcagcagc aagcccatca
    29341 tatttttttc catgcgtacg gcctggaaca gctcgccttt acggtcacgc cagtcctgcc
    29401 atttgctgcc ttcaggcagt ttttgctgac ttaatgagtc gactttcagc ggctcatcca
    29461 gccacaaacg ccagccggta atattgcctg ccggataacg catcagacgc gaggcatcct
    29521 caatattcac cagcatttca tagccatcga cttcactgtt ggcggcgaaa gtgccaatca
    29581 cattgaacag gcgctgactt ggaatacgcc ccatcggcgt gaactggcta gcagatggca
    29641 ccatcacgcg gatttgatca ccgcgattga cgcccaactg tgaggcaagc tgttcaccga
    29701 ggatgacatt atatttcccc ggctcgaggt cagtttgttt cacattgacc agatacggcg
    29761 ttagtggatc tttttgcgcc gggtcgatac ccagcatcac cccgaccgcc acgctgcgcg
    29821 cgctttgcag taccacatca ccggtagtaa taggtgcgac gcgattaacg ccgtccagtt
    29881 tgactgccgt ttccgggagt tgctgcgggt taagagagcc atgctcagaa gagagaattg
    29941 cctgtggcat caggccaagg atgttgtttt gcagctcgcg ctcaaagccg ttcatcactg
    30001 acaatactgt gaccagcgcc atcaccccga gggtaatgcc gatggtagaa agccaggaga
    30061 cgaaacgacc gaagcgatcc gctgcacgcc cacgcatgta acgcaggcca ataaatagag
    30121 cgacaggttg gtacatgaaa tccgtctggt tgctgtagca aagacacgag tatataagcg
    30181 aaagcaggat gggtaaacgt ctgaactgta agtgatcgtc agggctaatc tgaaaagtac
    30241 cagcaaatca atcggctaaa gtgcctcatt ttatcatcgg ccatgtggcc tgatctttat
    30301 ctgaaaaata accaaataca gacagatacg cattacacca cgcgactcgc cccgcaggat
    30361 caaccgcatg ttaaataaaa agagcgtgtt gtcccgatga aacaaaaaga gctatggatt
    30421 aaccagatca aagggttatg tatttgtctg gtggtgattt atcactcggt cattaccttt
    30481 tatccgcatc tgaccacttt tcagcatccg ttatcggaag tcctgagcaa atgctggatc
    30541 tatttcaatc tttaccttgc accctttcgt atgccggttt ttttctttat ctctggctat
    30601 ttgattcgcc gctatatcga cagcgtaccg tggggaaatt gtctcgataa acgcatctgg
    30661 aacatcttct gggtgctggc gctttggggc gtggtgcagt ggctggcgtt aagtgcgctg
    30721 aaccagtggc tggcacctga gcgcgattta agcaatgcct ccaatgccgc ttatgccgat
    30781 tccaccggtg agttcctgca cgggatgatc accgccagca ccagcttgtg gtatctgtat
    30841 gcgttaattg tctatttcgt gatatgtaaa atttttaacc ccctggccct gccgttattt
    30901 gttctgtttg tactgatgag tgtggctgtt aatttcgtac ccacaccgtg gtggggaatg
    30961 aacagtgtga tccgcaattt gccttattac agccttggcg catggtttgg cgcaacatta
    31021 atgacctgtg ttaaagcggt accgttgcgc cgccatctgc tgatggcttc tttgctgacc
    31081 gttctggcgg tcggtgcctg gttgtttaat atctcgctgc tgttgtcgct ggtatcgatt
    31141 gtggtaatca tgaagctgtt ttatcagtac gagcagcgtt tcggtatgcg ttcctccagc
    31201 ctgctgaatg tgattggctc caacaccatt gctatctata ccacccatcg cattctggta
    31261 gaaatattca gcttaagcct gattccgcaa atgaacgcgg tacgctggtc gccgcaagtc
    31321 gaactgacgc tcctgctgat ttaccccttt gtcagtttgt tgatttgtac tgttgcgggc
    31381 ttgctggtaa gaaaactttc acagcgcgca ttcagcgatc tgttgttctc cccgccttct
    31441 ctgcccgcta ccgtcagtta ctcccgctaa gccatcgccc cagccggggc gattcaattt
    31501 gctaaaccat gtcgattacg gataatgaac agcattgctt atcagagata tgccccatat
    31561 gttgaggcat atcctaacga gaatctgaca accgttatgc ctgaacaata tcgttatacg
    31621 ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt taaccggcgc tgcctgcgcg
    31681 acgctggtag cagaaattgc cgaacgtcac gccggtccgg tggtactcat tgcaccagat
    31741 atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt tcaccgatca gatggtgatg
    31801 aatctggcgg actgggaaac cctcccttac gacagttttt cgcctcatca ggacattatc
    31861 tcctcgcgtc tttccaccct ttaccagtta ccatcgatgc agcgtggcgt actgattgtt
    31921 ccggtgaata cgcttatgca gcgcgtttgc ccgcacagct ttctccacgg tcatgcgctg
    31981 gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac gaacccaact ggacagcgcc
    32041 ggttatcgcc atgttgacca ggtgatggag cacggcgaat acgccacgcg cggcgcgttg
    32101 ttggatctct tcccgatggg gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa
    32161 atcgacagcc tgcgggtgtt tgacgtcgat agccagcgca cgctggagga agtagaagcg
    32221 atcaatctgt tgcccgcgca cgaatttccg accgataaag cggcaattga actgttccgc
    32281 agccagtggc gtgatacctt cgaagtgaag cgcgacccgg aacatattta tcagcaagtg
    32341 agtaaaggca cattacctgc cgggatcgag tactggcagc cgctattctt cagcgaaccg
    32401 ctgccgccac tgttcagtta tttccctgcc aatacgctgt tggtgaatac tggcgatctg
    32461 gaaaacagtg ccgaacgttt ccaggctgac acgctcgcgc gttttgagaa tcgcggcatc
    32521 gacccaatgc gcccactgtt gccaccacaa tcgctctggc tgcgggtgga tgagctcttc
    32581 tcagagctga aaaactggcc ccgtgtgcag ctaaaaactg aacatttacc gacaaaagca
    32641 gcgaatgcca atttaggttt ccagaaactg ccagacctgg ccattcaggc acaacaaaaa
    32701 gcgccgctgg atgcgctgcg taagttcctc gagtctttcg acggtccggt ggtgttctcg
    32761 gtagaaagtg aaggccgccg tgaagcgttg ggtgaactgc tcgcgcgaat taaaattgct
    32821 ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg ggcgttatct gatgattggc
    32881 gctgcagagc atggttttgt cgatacggta cgtaatctgg cgctgatttg cgaaagcgat
    32941 ctgctcggtg aacgcgttgc ccgtcgtcgt caggattctc gccgcgccat caaccccgat
    33001 acactgatcc gtaaccttgc ggagctgcat attggtcagc cggtggtcca tctggagcac
    33061 ggcgtcggtc gttatgccgg aatgaccacg ctcgaagctg gaggcattac tggcgagtat
    33121 ttgatgctca cctatgccaa cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg
    33181 attagccgtt acgcgggtgg cgcagaagaa aacgcaccac tgcataaact tggtggcgat
    33241 gcctggtcac gcgcgcggca gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg
    33301 ctggatatct acgcgcaacg cgccgccaaa gagggcttcg cgtttaaaca cgatcgcgag
    33361 cagtatcagt tgttctgcga cagcttcccg tttgaaacca cgccggatca ggcgcaggcc
    33421 attaatgcgg tgcttagtga catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc
    33481 gatgttggct ttggtaaaac agaagtggcg atgcgcgccg ctttcatggc ggtagataac
    33541 cacaaacagg tggcggtgct ggtgcctacc acccttctcg cgcagcagca ttacgacaac
    33601 ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa tgatctcccg tttccgcagc
    33661 gccaaagagc agacgcaaat ccttgcggaa gtggcggaag ggaaaattga tattctgatc
    33721 ggtacgcaca aactgctgca aagtgacgtc aagtttaaag atttaggcct gctgattgtc
    33781 gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca ttaaagcgat gcgcgcgaac
    33841 gtggatattc tgacgcttac cgcaacgccg atcccacgta cgctgaatat ggcaatgagc
    33901 ggaatgcgcg atctgtcgat tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc
    33961 tttgtccgtg agtatgacag tctggtggtc cgggaggcga tcctgcgtga aattttgcgc
    34021 ggaggacagg tttattatct ctacaatgat gtggaaaaca tccagaaagc cgccgaacgg
    34081 ctggcagaac tggtacctga agcgcggatt gctatcggtc acgggcaaat gcgtgagcgc
    34141 gaactggaac gggtgatgaa tgatttccat catcaacgtt tcaacgtgct ggtttgtacc
    34201 accattatcg aaaccgggat cgacattcct accgccaaca ccattatcat tgaacgcgcg
    34261 gatcacttcg gtctggcaca gttgcaccag ttgcgcggtc gcgtcgggcg ttcgcaccac
    34321 caggcatatg catggctgct gacgccgcat ccaaaagcga tgactaccga tgcgcaaaaa
    34381 cgtcttgaag cgattgcctc gctggaagat ctcggtgcag gttttgcgct ggcaacgcac
    34441 gatctggaga tccgcggcgc gggtgaactg cttggcgaag aacaaagcgg ctcaatggaa
    34501 accatcggtt tctcgctgta tatggagttg ctggaaaacg ccgtcgatgc gctgaaagcc
    34561 ggacgcgagc cgtcgctgga agatctcacc agccagcaaa cagaagtcga gctgcggatg
    34621 ccgtcgctat tgccagatga tttcatccct gacgtgaaca cgcgtctgtc gttctacaaa
    34681 cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat
    34741 cgcttcggcc tgctgccgga tccggcgcgt accctgctgg atattgcccg tctgcgccag
    34801 caagcgcaga aactggggat caggaagctg gaaggtaatg agaaaggcgg cgtgatcgaa
    34861 tttgccgaga agaatcacgt taatccggcc tggttgattg gtttgctgca aaaacagccg
    34921 cagcattacc gccttgatgg tccgacgcgt ctgaagttta ttcaggattt gagcgagcgg
    34981 aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac tggaagagaa cgcgatcgct
    35041 taatggtaaa tcgggggcgt ttctgcgccc ccatctgcaa tatttacaaa ttctttgcac
    35101 ttccctgcat tatccgacac tgtcaccctc cataattcag acttaccttt ttattacaat
    35161 aaccttatat ttattatgga ctttggtgat gatcaaaacg cgtttttctc gctggctaac
    35221 attttttacg ttcgccgctg ccgtggcgct ggcgctaccg gcaaaagcca acacctggcc
    35281 gctgccgtca gcaggcagtc gtctggttgg tgaaaacaaa tttcatgttg tggaaaatga
    35341 cggtggttct ctggaagcca tcgccaaaaa atataacgtc ggctttctcg ctctgttaca
    35401 ggctaacccc ggcgttgatc cttacgtacc gcgcgcgggt agcgtgttaa cgatcccgtt
    35461 gcaaacccta cttccagacg cgccgcgcga aggcattgtg atcaacattg cggagctgcg
    35521 tctctattat tatccgccgg gaaaaaattc ggtaaccgta tatcccatcg gcattggtca
    35581 attaggtggt gacacgctga caccgacaat ggtgaccacc gtttctgaca agcgtgcaaa
    35641 cccaacctgg acgccaacgg caaacattcg cgcgcggtat aaggcacagg gaattgagtt
    35701 gcctgcggta gttccggctg gaccggataa cccaatgggc catcatgcga ttcgtctggc
    35761 ggcctatggc ggcgtttatt tgcttcacgg tacgaacgct gatttcggca ttggcatgcg
    35821 ggtaagttct ggctgtattc gtctgcggga tgacgatatc aaaacactct ttagccaggt
    35881 caccccaggc accaaagtga atatcatcaa caccccgatt aaagtctctg ccgaaccaaa
    35941 cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag aagattgatg acgatccgca
    36001 gctgctgcca attacgctga atagcgcaat gcaatcattt aaagatgcag cacaaactga
    36061 cgctgaagtg atgcaacatg tgatggatgt ccgttccggg atgccggtgg atgtccgccg
    36121 tcatcaagtg agcccacaaa ctctgtaaag ctcggataaa aaaatccccg cgacacggca
    36181 tgtcgcgggg aaaaaaacaa gcagtagcag gcattaatga gggttaatgc ttatttataa
    36241 attactgctg ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt
    36301 gcgcccatct catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc
    36361 gcgttagcac tgatagtacc gactttttgt tggccttctg gcgttgactg aacttcgaca
    36421 gccgcaaagc tggcaaatga catggagctt aaaatcgccg cagcgatgag ggtttttacg
    36481 tttttcataa tagtggcctt atgcagatga atgacgtcgc aagcaattac ttaacg
//
LOCUS       DACADF010000030        36528 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.030, whole genome shotgun sequence.
ACCESSION   DACADF010000030 DACADF010000000
VERSION     DACADF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.030"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(90..377)
                     /locus_tag="ISB30_001457"
     CDS             complement(90..377)
                     /locus_tag="ISB30_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989103.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(452..595)
                     /gene="mgrB"
                     /locus_tag="ISB30_001458"
     CDS             complement(452..595)
                     /gene="mgrB"
                     /locus_tag="ISB30_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAP1989104.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            754..993
                     /locus_tag="ISB30_001459"
     CDS             754..993
                     /locus_tag="ISB30_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989105.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1137..1928)
                     /gene="kdgR"
                     /locus_tag="ISB30_001460"
     CDS             complement(1137..1928)
                     /gene="kdgR"
                     /locus_tag="ISB30_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAP1989106.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTIISTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2105..3478
                     /locus_tag="ISB30_001461"
     CDS             2105..3478
                     /locus_tag="ISB30_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1989107.1"
                     /translation="MPKVQADGLPLPLRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3524..4405)
                     /gene="htpX"
                     /locus_tag="ISB30_001462"
     CDS             complement(3524..4405)
                     /gene="htpX"
                     /locus_tag="ISB30_001462"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAP1989108.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4597..6645)
                     /gene="prc"
                     /locus_tag="ISB30_001463"
     CDS             complement(4597..6645)
                     /gene="prc"
                     /locus_tag="ISB30_001463"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAP1989109.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6665..7363)
                     /gene="proQ"
                     /locus_tag="ISB30_001464"
     CDS             complement(6665..7363)
                     /gene="proQ"
                     /locus_tag="ISB30_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAP1989110.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKSGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7460..7957)
                     /locus_tag="ISB30_001465"
     CDS             complement(7460..7957)
                     /locus_tag="ISB30_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP1989111.1"
                     /translation="MNKAEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8087..9370
                     /gene="yebS"
                     /locus_tag="ISB30_001466"
     CDS             8087..9370
                     /gene="yebS"
                     /locus_tag="ISB30_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAP1989112.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGAMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLLLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9339..11972
                     /gene="yebT"
                     /locus_tag="ISB30_001467"
     CDS             9339..11972
                     /gene="yebT"
                     /locus_tag="ISB30_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAP1989113.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPGGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12052..13491
                     /gene="rsmF"
                     /locus_tag="ISB30_001468"
     CDS             12052..13491
                     /gene="rsmF"
                     /locus_tag="ISB30_001468"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAP1989114.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPHAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13608..13844
                     /locus_tag="ISB30_001469"
     CDS             13608..13844
                     /locus_tag="ISB30_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAP1989115.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13865..14140
                     /locus_tag="ISB30_001470"
     CDS             13865..14140
                     /locus_tag="ISB30_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAP1989116.1"
                     /translation="MAGYLSGLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(14141..14797)
                     /gene="pphA"
                     /locus_tag="ISB30_001471"
     CDS             complement(14141..14797)
                     /gene="pphA"
                     /locus_tag="ISB30_001471"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAP1989117.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15192..15533)
                     /locus_tag="ISB30_001472"
     CDS             complement(15192..15533)
                     /locus_tag="ISB30_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAP1989118.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15546..16418)
                     /gene="copD"
                     /locus_tag="ISB30_001473"
     CDS             complement(15546..16418)
                     /gene="copD"
                     /locus_tag="ISB30_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HAP1989119.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPQNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTHWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16422..16796)
                     /gene="yobA"
                     /locus_tag="ISB30_001474"
     CDS             complement(16422..16796)
                     /gene="yobA"
                     /locus_tag="ISB30_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAP1989120.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16935..17165
                     /locus_tag="ISB30_001475"
     CDS             16935..17165
                     /locus_tag="ISB30_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAP1989121.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17267..17923
                     /locus_tag="ISB30_001476"
     CDS             17267..17923
                     /locus_tag="ISB30_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAP1989122.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17947..18609
                     /gene="exoX"
                     /locus_tag="ISB30_001477"
     CDS             17947..18609
                     /gene="exoX"
                     /locus_tag="ISB30_001477"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAP1989123.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18606..20666)
                     /gene="ptrB"
                     /locus_tag="ISB30_001478"
     CDS             complement(18606..20666)
                     /gene="ptrB"
                     /locus_tag="ISB30_001478"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAP1989124.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVDPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEIADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20875..21534)
                     /locus_tag="ISB30_001479"
     CDS             complement(20875..21534)
                     /locus_tag="ISB30_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAP1989125.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDLQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(21861..22217)
                     /gene="yebF"
                     /locus_tag="ISB30_001480"
     CDS             complement(21861..22217)
                     /gene="yebF"
                     /locus_tag="ISB30_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAP1989126.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22284..22574)
                     /gene="yebG"
                     /locus_tag="ISB30_001481"
     CDS             complement(22284..22574)
                     /gene="yebG"
                     /locus_tag="ISB30_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HAP1989127.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22708..23886
                     /gene="purT"
                     /locus_tag="ISB30_001482"
     CDS             22708..23886
                     /gene="purT"
                     /locus_tag="ISB30_001482"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAP1989128.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23942..24583)
                     /locus_tag="ISB30_001483"
     CDS             complement(23942..24583)
                     /locus_tag="ISB30_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP1989129.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPIAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24620..26431)
                     /gene="edd"
                     /locus_tag="ISB30_001484"
     CDS             complement(24620..26431)
                     /gene="edd"
                     /locus_tag="ISB30_001484"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAP1989130.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26666..28141)
                     /gene="zwf"
                     /locus_tag="ISB30_001485"
     CDS             complement(26666..28141)
                     /gene="zwf"
                     /locus_tag="ISB30_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP1989131.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28479..29348
                     /locus_tag="ISB30_001486"
     CDS             28479..29348
                     /locus_tag="ISB30_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP1989132.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29476..30918
                     /gene="pyk"
                     /locus_tag="ISB30_001487"
     CDS             29476..30918
                     /gene="pyk"
                     /locus_tag="ISB30_001487"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP1989133.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31050..32021)
                     /gene="lpxM"
                     /locus_tag="ISB30_001488"
                     /gene_synonym="msbB"
     CDS             complement(31050..32021)
                     /gene="lpxM"
                     /locus_tag="ISB30_001488"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAP1989134.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32140..33462)
                     /gene="mepM"
                     /locus_tag="ISB30_001489"
     CDS             complement(32140..33462)
                     /gene="mepM"
                     /locus_tag="ISB30_001489"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAP1989135.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33478..34410)
                     /gene="znuA"
                     /locus_tag="ISB30_001490"
     CDS             complement(33478..34410)
                     /gene="znuA"
                     /locus_tag="ISB30_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAP1989136.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            34489..35244
                     /gene="znuC"
                     /locus_tag="ISB30_001491"
     CDS             34489..35244
                     /gene="znuC"
                     /locus_tag="ISB30_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAP1989137.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35241..36026
                     /gene="znuB"
                     /locus_tag="ISB30_001492"
     CDS             35241..36026
                     /gene="znuB"
                     /locus_tag="ISB30_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAP1989138.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(36220..>36528)
                     /locus_tag="ISB30_001493"
     CDS             complement(36220..>36528)
                     /locus_tag="ISB30_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1989139.1"
                     /translation="EKPLLHSDQGWHYRIKSYQSALADRGLVQSMSRKGNCLDNAVME
                     NFFGHLKEEMYYRRDYRNVEELENAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG
                     "
BASE COUNT         8727 a         9379 c         8985 g         9437 t
ORIGIN      
        1 atttgaagca gttgcaccat gacagtgcgt ataatcagca tgaattgagt aacccttcaa
       61 cgacggggtt actcaagatt gaggaaggat tatcgttcgg cgaccagacg aataatatct
      121 tcgtcttcaa ccgatttttg actctcaacg gtctttttat ctgctttctc aggttcgttt
      181 gcttcgcaca aacgacgtaa cagtgcattt tgacgttttt gctgatccag caacgcctca
      241 agcagttcaa tctgttcgtt agtccgtgaa ctggcacgat tgataaaaaa ccacaagatg
      301 agcccgataa gaagaaccac caccgataca accaaagacg caatgttcat cacgcctgaa
      361 tttacaactt cgttcatttc accacctctg agtagaggcg ctattctacc actgctggag
      421 aggaagaaaa tccagtgctg aaaaaatgat atcaccacgg gataaactgg ttaatggcac
      481 aaattccgct gaaaaattgt acatcctgat cgcacatcat gttgaatacc tgcgcccaaa
      541 gcagcaagca agccaacacc acgacaacca gagcgaccca tcgaaacttt ttcactccaa
      601 tctccgtttc acctacctta tgtcattaaa ctagcatgcg ttagttaaac agcgcctaac
      661 tatgtcgata ttcgtgcttt ttcagaatga gtcgcttgct tcagcgcata ttgccgctac
      721 gattaagcga acataaaaag agaagaggtt gtaatgcgat tcatcattcg cacagttatg
      781 ctgatagcac tggtatggat aggattatta ctcagcggat atggcgtttt gattggaagc
      841 aaagagaatg ccgcgggatt aggtttgcaa tgtacctatc tgaccgccag aggcaccagc
      901 acggtacaat acctgcacac taaaagtggt tttctgggga taacagattg cccgttgtta
      961 cgcaaaagca atattgtggt cgataatggt taagggtaat aaataaccag attctcactc
     1021 atgagtaaaa tattcttatc gcaagtgcgt ttaatataac gccaccttca actttatttt
     1081 tctcatgcta aataaagaac ctgtctgttt gatattcaga caggttctta ctctcatcag
     1141 aacggatagt cgtgataacc catttgggca gaaattttgc gcgccgcggt atgcaacatt
     1201 gcgacatatt cctgtaaacg ctcttcagag aaacgcaacg tcgggaagga gatgctcaga
     1261 ccggcaatga ccacgccaaa gcgatcaaat accggaaccg caatgcaacg cagtccttct
     1321 tcctgctctt cgttatcttc gccatacccc tgctcgcgca cctggtccag aacgggtaat
     1381 aacgcttctg tactgatgat ggtccgttcg gtactgcgtt tatactccac gccctcaaga
     1441 atttgcatca cttcatcgcg atcgcgccat gccagcagta ccttaccaat cgcggtgctg
     1501 tacagcggat tacgacgccc aatccgtgaa tacatgcgca aattgtacat tgagtcaatt
     1561 ttgtggatgt aaacaatact gtcttcgtcc agtgcgccga ggtggatagt ttctttggtc
     1621 aggcgggaga tctcacgcat ctggatatct gcgctacgaa ttaaatcgac gttttgcaac
     1681 gcgcgggcgc ccagttcaaa caatttcagg gtcagcgaat atttctccga ctccccttcc
     1741 tgcgccacat aacctaaggt tttcatggtc tgtaaaaagc gataaacggt gctttttgac
     1801 atcatgacgc gctgcgacaa ctcggttatc cctatttcgc gctcttcacc cagcgcctgc
     1861 aaaatgccaa aaacttttag cacggaagat acagaatcag gctgtttatc cagatctgcg
     1921 ttagccattt atcacctcat tgcgagtgtt ttataaaaat cagaactgtt ttttattata
     1981 atttcgcacc tgggtggtcg caatccatct tttgccggtt agttacaatt ctgcgacatc
     2041 caccgtgaat atcagtgcta gaatcatacc cctgttgatt attcaccaaa gatataaaat
     2101 tcctatgcca aaagttcagg ccgacggcct gccattgccc ctgcgatacg gtgcgatatt
     2161 aaccattgtg attggtattt cgatggctgt ccttgacggc gcaatcgcca acgtcgcctt
     2221 gccaacaatc gctacggacc ttcatgccac gccggccagt tccatctggg tagtgaacgc
     2281 ctatcaaatc gccattgtca tctccctgct ctcgttttcg tttctgggcg atatgtttgg
     2341 ctatcgacgt atttataaat gcggtctggt cgtttttctg ttgtcttcac tgttctgcgc
     2401 cctttctgat tcgctgcaaa tgctcaccct tgcgcgtgtc atacaaggtt tcggcggtgc
     2461 agcgttgatg agcgttaata ccgcacttat ccgcctgatc tatccacaac gttttctggg
     2521 tagagggatg ggcataaact cgtttattgt tgccgtctct tctgctgccg ggccgacaat
     2581 tgctgcagca atcctctcca tcgcatcctg gaaatggtta tttttaatca acgtaccgtt
     2641 gggtattatc gccctgcttc tggcgatgcg ttttctgcca cccaatggtt ctcgcgccag
     2701 taaaccccgt ttcgacctgc ccagcgccgt gatgaacgcg ttaaccttcg gcctgcttat
     2761 tactgcattg agtggtttcg ctcaggggca atcgctgaca ttgattggtg cggaactggt
     2821 ggtaatggtt gtcgttggta ttttctttat tcgccgccag ctttctcttc ccgtaccgct
     2881 gctaccggtg gatttactgc gtatcccgct gttttcactt tctatttgca catctgtttg
     2941 ctctttctgc gcacaaatgc tggcaatggt ttcccttccc ttttacctgc aaaccgtgct
     3001 cgggcgtagt gaagtcgaaa caggtttact tctgacaccg tggccgttag caacgatggt
     3061 tatggctcca ctggcaggct atttgattga acgcgtacat gcaggattgc tgggtgcttt
     3121 agggttattc atcatggctg cggggctttt ttccctggtt ctgctgccag cgtcacctgc
     3181 ggatatcaat attatctggc cgatgatctt atgtggtgct ggatttggct tgttccagtc
     3241 acccaataac cacaccatta ttacctccgc tcctcgcgaa cgtagcggtg gagccagtgg
     3301 catgttaggg acggctcgtc ttctgggtca gagtagcggc gcggctctgg tagcgctgat
     3361 gctaaatcag tttggtgata atggtacgca cgtctcgctg atagctgcgg ctattctggc
     3421 agtgattgct gcctgtgtca gtggtttacg tatcactcag ccacgatcca gggcataaaa
     3481 aaagcgcgtc gatcaggacg cgctttttag tatttacgtc atattacttc aggtattcac
     3541 ccgtacgcag agcttcaata cgtttatcca gcggcgggtg ggtcatgaac aactcactga
     3601 gcgatttcga cttaccgtta atgcagaaag ccatcatgct ggttgcttct tgcggttcat
     3661 agctggtttt caggcgttgc agcgcggcaa tcattttctc gcgaccaacc agttttgctg
     3721 aacccgcatc agcatggaat tcacgatgac gcgagaacca catggtgata atgctcgcca
     3781 gaataccaaa caccagttcc agaaccgttg caaccgcaaa gtagatcagc gggttgccgt
     3841 tgctctcttc accttcatca cggtttccgc ccataaaacc cgcggcaagc tgcgccagaa
     3901 tacgggaaat aaagataacg aaggtgttca ccacgccctg aatcagcgtc atggtgacca
     3961 tatcaccatt ggcgatgtgg ctgatttcgt gagcaattac cgcctcggct tcatccgggc
     4021 tcatgttctg cagcaaacca gtgctgacag caaccagaga ggcatcacgg cgcgcaccag
     4081 ttgcaaaagc attgatgtcc ggcgcatggt agatagccac ttgcggcata gcgatccccg
     4141 cctgacgagc ctgggttgct acagtattga ccagccaacg ttccctttcg ttacgcggtt
     4201 gctcgatcac ttccccgcca acagatcgta atgccatcca tttggacatc agaagcgaaa
     4261 cgaaggaacc accaaaaccg aacagcaagg ccatgatcat cagcccctga acgctgctcg
     4321 actgtatccc tgtcaggctc agtaccagcc cgaaaacgac cattacggcc aggttcgtta
     4381 gcaggaagag cgcgattcgc atcataattt tctttttacc tcagtttaac aaaacgcaat
     4441 atgcgatacc cacatcgtat gggttacgcg actattttca agtctggata gtgcgtaagt
     4501 caccagaaag acacaacttt acattttgta gcatctgatt tacggcatct tgccgctgtt
     4561 aaaaaatcag gcacaatttc ttgtgcctga tagatattac ttgacgggag cgggttgttc
     4621 cgcgggtctg gctttttcaa gcttcgccag atcgagtgcg atattcaccg tctcatccag
     4681 ataaggatct ggctcctggt aatctttcgg tagatcatcc agtttcttca actccggttt
     4741 accttcgcgt ttaaagcgtt cgttcaaacg cgccagacgc gtcgcatcat cttcattatt
     4801 ctctttctca cgcacagcgt aattcagaga aacgatattg cgcttgtcct tcatagcgtt
     4861 gaagcgcgcg atatccttca tgatgttctg gaactcagga tctttcgcga tacgcgcatt
     4921 atgttccttc agcagctccg gttcaaaggc cgttaaatct cctgatttca cataagtcgc
     4981 ggcatcaatg ctatcccacg gcagcgcgtt atcttcgaat ttctcacccg tttccgtttc
     5041 ttcattaccc gtcggcatga tgatgtctgg cgttacgcct ttacgttgcg tactgccgcc
     5101 gttaacgcga tagaacttct ggatcgtgta ctgcacagaa cccagcgctg gccattcagg
     5161 acgtaacatc tgatcgtaaa tacggttcaa tgaacggtac tgctgaacgg tgcctttacc
     5221 aaacgtcggt tcacccacaa ccagggcacg accgtaatcc tgcattgccg cggcaaagat
     5281 ttctgaagcc gaagcactga agcggtcaac cagcaccacc agcgggcctt tatagaagac
     5341 ctgtccgtcg gtgtcgctgt cttcacgaac cttgccgttg ttatcgcgga cctgaacaat
     5401 cgggccggat ggaatgaaca gaccggagag cgatacggct tcagtcaacg ccccaccacc
     5461 attgctacgc aggtcgataa tcacactgct gacattctgt ttttccagtt tctgcagttg
     5521 cactttgaca tcgtctgtca aacccacata gaagcctgga atatccagca cgccgacttt
     5581 ctctttaccg acggtcttca ccgacatttt aaccgcgcgg tcttcgagac gaatacgttc
     5641 acgggtcaac gttacagtac gcgtcttggt ccctttacca gcaggtaaaa tttccagacg
     5701 aactttactg cccttaggcc ctttaattaa ggccaccaca tcatcaagac gccagccaat
     5761 cacgtcaacc atcggcttgc ctgtttgacc aacaccgaca attttgtcac caacgctgat
     5821 agctttgctc ttcgctgccg gacctcctgc caccatcgaa ttgataacgg tgtagtcatc
     5881 atccatttgc agcactgcgc caataccttc cagcgacaaa ctcatttcag tgttgaactg
     5941 ttcggtatta cgcggggaaa gatagttggt atgcgggtcg atttcacgtg caaacgccgt
     6001 cattgccagc gagaaaacat cttcgctgtt ggtttgcgcc agacgacgaa tggcaaattt
     6061 gtagcggcga gtcagggttt cacgaatttc cttatccgtt tttcctgtca gcttcaggct
     6121 taactcgtcg aatttgactt tactgtccca cagcgcgttc aactcagcct cgtttttcgg
     6181 ccagggcgct ttgctgcggt caaggttata agtgtcgttg ccggtgaaat ccatcggctt
     6241 ttccagtacc gacaaagcgt actggtaacg ttcaaaacgg cgcttttgcg ccagattgta
     6301 gagatcgtag aaaacgtcga gtttacctga acgcagttca tcgcctaact cagttttctt
     6361 tttcgcgaac tgttcaacat cgcttgccag cagcacatta tggctgtaat cgagcagatt
     6421 caggtagcgg tcaaagattt tggccgaaaa tgcctgatcg aggtcgaact ggcgataatg
     6481 agaacgggtg aagcgcgacg ttacgcgctc actcaccgtc gcatgctgcg tctcttcctt
     6541 taataccgga atttgatcag cacgcgtgat atcttctaca gcgaaggtct ggcctgctat
     6601 tgcaagcagg ccagctaacg cggtaagcct aaaaaacatg ttcatgcctg gcccggcctc
     6661 cgtttcagaa caccaggtgt tctgcgcgca caatcaaaga catacccgaa ttcagctgga
     6721 cgcggacgcc gtctttggtg atttctaata cggtggcatc catcgcgttt tgacccgctt
     6781 tcaccttcag ggcttgtccg acagtcagag ctgaaatgtc agaaaccggg gtgtgctgtt
     6841 cttcgcgagg tgcttttact gtttttggcg ctttctctac cggcttttgc gcacgaggtt
     6901 tacgttcagc gccttctttg cggcgtggcg tggtcgggcg tggcttgcgt tcgcggcgcg
     6961 gtgcgtcttc tttctcacca gcagctgcgg cagcttcgcg ttttttcgct tgctgttcag
     7021 cacgctgtgc ctgaacacgc gctttcgctt cttcaagctg cttgcgagca tgctcgacat
     7081 gttgctcgtc cagctcaccg catgggttgc cgtcaagatc aacacgcgtt gcgccggatt
     7141 taacaccgta aagataacgc cagctcgaag tgtagagacg taaagcggat cgcaattgcg
     7201 ttttgctcag gttcatttcc ccggcaacac gatcgaccaa atcctgaaaa ataccgattt
     7261 tcagcggacg cgcttcacct tccgcactga aacagtgggg aaaacgttcg gccagaaacg
     7321 cgattacttc tttactgcta ttcaacttag gttgattttc catgaaattt cctgattaca
     7381 acggacgtag ccaacaagcg caggcatgaa caggcgtcat tataatgacg ctatcagtaa
     7441 atgctacgtt atccgttgat tatcctgcga cgctcgcaaa gaattttttg taatccgtcg
     7501 ttgcaagcac tttttcaagc tgtgccacaa gctgacgtaa gccttgctcg tcctcgtctg
     7561 taaagcgacc gaagacggta ctatcgatgt cgagaacacc aataatctga tttttcacca
     7621 ccagtggcag aacaatttca gaattactcg ccgcatcaca ggcaatatgc ccgtcaaacg
     7681 cgtgaacatc ctcgatacgc tgcacttgat tgcgggcaac cgcagtgccg catacgccgc
     7741 gcccaacggg tatccggaca caggcaattt tgccctgaaa tggtccgagc accagtgtat
     7801 cgtcctcaag caaataaaaa cctgcccagt ttacgtcagt gagacgctca tataacaacg
     7861 cactggtgtt cgcaagcgtt gccagaaaac tggtttctcc cgccatcagc gcgttaaagt
     7921 cgcgatttaa atccgcgtaa aattctgctt tgttcattat ataatcactt ggttgtctta
     7981 cctggaactg ccagcctatt aaaataagca ttaaatgcgt taatgctcaa gatcattccc
     8041 atcatgggtt aagattaatg ttaattctta ttacatttgg cacgtcatgg ctcttaacac
     8101 accacaaatt acgccgacaa agaagataac agtgagggct atcggcgagg aactgccgcg
     8161 tggtgattac caacgttgcc cgcaatgtga catgctgttt agcctgcccg agataaattc
     8221 tcatcaaagt gcctattgtc cgcgctgtca ggcaaaaatt cgtgacgggc gcgactggtc
     8281 gctaacgcgc ctggcggcaa tggccttcac catgctgttg cttatgccgt ttgcctgggg
     8341 cgaaccgctg ttgcatatct ggctgttagg cattcgtatt gacgccaacg ttatgcaagg
     8401 catctggcaa atgaccaaac agggcgatgc gataacgggg gcgatggtct ttttctgcgt
     8461 tatcggtgcc cccctcattc tggtgacctc catagcttat ttatggtttg gtaaccgatt
     8521 gggaatgaat ttacgcccgg tactgctgat gcttgagcgg ctgaaagagt gggtgatgtt
     8581 ggatatctac ctggtcggca ttggcgttgc ttctataaag gtacaggatt atgcccatat
     8641 ccaggcgggg gtcggcttgt tctcttttgt ggcgttggtg attttaacga cggtgacgtt
     8701 gtcacatctt aatgtcgaag aactgtggga gcgattttat ccgcagcgcc ccgctacgcg
     8761 aagggacgag aaactccgtg tctgtcttgg gtgccatttt accggctatc ctgatcagcg
     8821 tggtcgctgc ccgcgttgcc atatcccgct actcctgcgt cgccgtcata gtctgcaaaa
     8881 atgctgggcg gcgctgttag cgtcaatcgt tttgttgtta cctgccaacc tgttgcctat
     8941 ctctatcatt tatctgaatg gtggtcggca ggaagatacg attctttccg gaattatgtc
     9001 gctggcaagt agcaacattg cagttgcggg aatcgtgttt atcgccagta ttctggtacc
     9061 gtttactaaa gtgatcgtca tgttcacttt actgttgagt attcatttta aatgccagca
     9121 aggtttacgc acacgcattc tgttactgcg gatggtgacc tggattggtc gctggtcgat
     9181 gctcgacctg tttgtcatat ctttaaccat gtcgctgatt aatcgcgatc agatcctcgc
     9241 ttttactatg ggaccggctg cgttttattt cggcgcagcg gtaattttga ctattcttgc
     9301 tgtggaatgg ctggacagcc gcttactttg ggatgcacat gagtcaggaa acgcccgctt
     9361 cgacgactga agcgcagatt aaaaataaac gccgtatctc acctttctgg ctgctgccgt
     9421 tcatcgcgct aatgattgct ggttggctga tttgggacag ttatcaggac cggggcaata
     9481 ctgtcaccat cgactttatg tcggcggatg gtattgttcc aggccgtacg cctgttcgtt
     9541 atcagggcgt tgaagttgga acagtgcagg atatcagcct cagcgacgat cttcgtaaga
     9601 ttgaagtcaa ggtcagcatc aagtccgata tgaaagatgc gctgcgcgaa gagacacagt
     9661 tctggttggt gacgccaaag gcctcgttgg caggtgtctc cgggctggac gccctcgtcg
     9721 gtggtaatta tatcggcatg atgccgggta aaggtaaaga gcaggatcac tttgtcgcac
     9781 tcgacaccca accgaaatat cggctggaca atggcgatct gatgatccac ctgcaagccc
     9841 ccgatctcgg ttcactgagc agtggttcat tggtctattt ccgcaagatc ccggtgggta
     9901 aagtctacga ctatgccatc aatcccaaca agcaaggcgt ggtgattgat gtcctgatcg
     9961 agcggcgttt taccgatctg gtgaaaaaag gtagccgttt ctggaacgtt tccggcgttg
    10021 atgccaacgt cagtatcagt ggcgcgaagg tgaaactgga aagtctggcg gcactggtta
    10081 acggtgcgat tgccttcgat tcacctgaag agtcgaaacc tgccgaagcg gaagatacct
    10141 ttggtctgta tgaagacctg gcccatagcc aacgtggcgt aataataaaa ctggaactgc
    10201 caggcggtgc cggattaacc gccgactcga cgccgttaat gtatcagggg ctggaagtcg
    10261 gacagctgac taaactggat ttaaatcctg gtggtaaagt cactggggaa atgaccgttg
    10321 atcccagcgt cgtcaccctg cttcgtgaaa atacccgcat cgaattacgt aatccaaaat
    10381 tatcccttag cgatgctaat ctcagtgccc tgctgaccgg caaaaccttc gaactggtgc
    10441 ccggcgatgg cgagccacgt aaggagttcg tggttgtgcc aggcgaaaaa gccctgctgc
    10501 aagagccgga tgttctgaca ctgaccctga cagcaccaga aagttacggt attgatgctg
    10561 gtcagccgct cattcttcac ggcgtgcagg taggccaggt tatcgatcgt aaactcacca
    10621 gcaaaggcgt cacctttacc gtcgccatcg agcctcagca tcgcgaactg gtaaaaggcg
    10681 atagcaaatt tgtcgtcaac agccgtgtcg acgtgaaggt ggggctggat ggcgttgagt
    10741 ttctcggtgc cagcgcctca gaatggatca atggcggaat acgtattctg ccgggcgata
    10801 aaggcgagat gaaagccagc tatccactgt atgccaatct ggaaaaagcg ctggagaaca
    10861 gccttagcga tttacccacc acaaccgtga gtttgagtgc agagacgctg ccggatgtgc
    10921 aggcaggatc ggtagtgctg taccgtaaat ttgaagttgg tgaagttatt actgtgcgtc
    10981 cgcgagctaa cgcgtttgat atcgatctgc atattaaacc ggagtatcgc aaccttctga
    11041 ccagcaacag cgtgttctgg gcagaaggtg gggcgaaagt tcagctgaat ggtagtggcc
    11101 tgaccgtaca ggcatccccg ctctccagag cattaaaggg ggctattagc ttcgataacc
    11161 tcagcggtgc cagcgccagt cagcgtaaag gcgacaagcg tattctgtat gcttccgaaa
    11221 cagcggcccg tgcggttggc gggcaaatta cgcttcacgc tttcgatgcc ggaaaactgg
    11281 cggtcgggat gccaattcgc tatctcggta ttgatatcgg gcaaatccag acgctggatc
    11341 tgattaccgc gcgcaatgaa gtacaggcaa aggcggtgct ctatccggaa tatgtccaga
    11401 cctttgctcg cggtggtacg cgcttctcag tggtcacacc gcaaatttcg gcagctggcg
    11461 ttgagcatct tgatactatc ctccagccgt atatcaacgt cgaaccaggc cggggcaatc
    11521 ctcgccgcga ctttgaatta caagaggcca ccattactga ttcgcgttac ctggatggct
    11581 taagcattat tgttgaagcg ccggaagccg gttcgttagg tatcggtaca cctgtgctgt
    11641 tccgtggtct ggaagtaggt acggttaccg gaatgacgct ggggacattg tcagatcgcg
    11701 tgatgattgc gatgcgcatc agtaaacgct atcaacacct ggtgcgtaac aattccgtct
    11761 tctggttggc atcgggttac agtctggact ttggtctgac gggcggcgta gtgaaaaccg
    11821 gcacctttaa ccagtttatc cgtggcggca tcgccttcgc cacgcctccg ggtacgccac
    11881 tggcaccgaa agcccaggaa ggcaagcact tcctgttgca ggaaagtgaa ccgaaagagt
    11941 ggcgtgaatg gggaactgcg cttcccaaat aatgcccact gctccggcgt gcctgcgccg
    12001 gagcgtttat gctaaactgc acgcctgttt ttttgccagt ggtatatgct cgtggcccaa
    12061 cacaccgttt atttcccgga cgcctttctg acacaaatgc gcgaagcgat gccttcgacg
    12121 ctctcttttg atgattttct tgccgcctgt cagcgcccgt tgcgccgcag cattcgcgtt
    12181 aatacgctga aaacctccgt tgctgatttc ctgcaattaa ccgctcctta tggctggacg
    12241 cttacgccaa ttccgtggtg tgaagaaggt ttctggattg aacgcgacaa tgaagatgca
    12301 ttgccgttgg gtagtaccgc cgagcattta agcggcctgt tttatattca ggaagccagt
    12361 tcaatgttgc ctgttgccgc cttgtttgct gacggtaatg caccacagcg ggtgatggat
    12421 gtcgctgctg caccaggttc caaaacgacg caaattgccg cgcggatgaa taacgaaggg
    12481 gcaatccttg ccaatgagtt ttccgccagt cgggtaaaag tgttacatgc caatatcagc
    12541 cgctgtggca tcagtaatgt tgcgctcacg cattttgatg gccgcgtatt tggtgcggca
    12601 gtgccagaaa tgttcgatgc cattttgctg gacgctccct gctccggcga aggcgtggtg
    12661 cgtaaagatc ccgatgcgct aaaaaactgg tcaccagaaa gcaatcagga aatcgcagcg
    12721 acccaacggg agctgatcga cagcgccttt catgcattac gtcctggcgg tacgctggtt
    12781 tactcgacct gtaccttaaa ccgggaagaa aacgaagccg tttgcctgtg gctgaaagag
    12841 acttaccccg acgcagtaga gtttttaccg cttggcgatc tcttccctgg tgcaaacaag
    12901 gcgctgaccg aggaaggctt tttgcatgtt ttcccacaaa tttacgactg cgaaggcttc
    12961 ttcgttgctc gtctgcgtaa aactcaggcg atccccgtct tacccgcccc aaaatacaaa
    13021 gtgggcaatt tcccgtttag cccagtgaaa gatcgcgaag ccggtcaaat tcgtcaggcg
    13081 gctgcaagtg ttggcttaaa ctgggatgaa aacctgcgac tctggcagcg tgacaaagaa
    13141 ctgtggttgt tcccggtggg cattgaagcc ctgatcggta aagtccgatt ttctcgcttg
    13201 gggattaaac ttgccgaaac gcacaacaaa ggttatcgct ggcagcatga ggccgttatt
    13261 gcccttgcct cccccgacaa tgtgaacgct tttgaactga caccgcagga agcggaggag
    13321 tggtatcgcg ggcgcgatgt atacccgcat gccgcgccag tggcggatga tgtattggtt
    13381 actttccagc atcagccgat tggtttagcc aaacggattg gttcacgact gaaaaacagc
    13441 tacccgcgtg aactggtgcg cgacgggaaa ctttttaccg gtaacgcctg acagcgcaca
    13501 aaaaagcgca ctttttgact ggcacattcg actgcctcaa ctaggctgaa aaatggtgcg
    13561 atcggactgg tcgtaccaca accggcagct aaatggagag cacgaagatg aaaaccagtg
    13621 tgcgcatagg cgcttttgaa atcgacgacg gcgaattaca cggcgaatcg ccgggtgatc
    13681 gaacgttaac cattccttgt aaatcagacc ccgatttatg tatgcaactg gatgcctggg
    13741 atgctgaaac cagtatccct gccctgctaa atggcgaaca ctctgtcctt taccgtaccc
    13801 gttacgatca acaatctgat gcctggatta tgcgtcttgc ctgatccaaa aagaacccgt
    13861 cggcatggcg ggttatttgt ccgggttatt cccccgttgt aaaatctctc ctaaacttaa
    13921 cggtacggca ccacacttcg gggatgaaat gttcgcgctg gtactttttg tttgctacct
    13981 ggatggcggt tgtgaagata ttgttgtgga tgtctacaac acggaacagc agtgtcttta
    14041 ttctatgagc gatcaacgga tccgccatgg cggttgtttt ccgattgagg attttataga
    14101 tggtttctgg cgacctgcac aggagtacgg tgatttttaa ttattgcaat tgcacaagag
    14161 tcagttctcc cccaaagaca gcaccggtat caatataatg caggttgcca atatccacgc
    14221 gatggcgcaa aggtgtatga ccaaaccaga aatgatcagc acctgcaatt ccctgccctt
    14281 tttggcgttc acctaatcgc gagcggctcc acaagacctg atgcaaatcg acgtcctttt
    14341 gccattcata aacatcatct ggataatcgg catgagcaat aacatgtttg ccggtgcggc
    14401 tgtgtacttc aagaataaag ggcaaatgct gacatttttc cagcgccgtt ttcgcttgtt
    14461 tctgttgatt atctgccagc gcaataaacc agtcgccgcc attcatcaac cataaagaca
    14521 tctgccggga tgccagcgca tccatcgcca tctgttcatg attgcctctt accgcacgaa
    14581 cccaatgttg ttccagtaac tgcaggcaac gtaaactttg cggcccacga tcgataacgt
    14641 ctcccactga gataagtaaa tctcgccacg gatcaaaacg acaatgccat aatttgcggc
    14701 gcaattgctc aagacaaccg tgtatatcgc ctgaaagcca gatatgtcgc cattgatgac
    14761 ccgcaattct ctgataaacg ggcgcaggct gtttcatcaa tattttcctc ccgcgctaaa
    14821 gatcacataa tcttaacaag aatgttaaaa aacgctggac tcaaacagta gagtgtgtgt
    14881 tatggttgac tataaagtca gcgaagggaa tgcttctggc ttttaacaga taaaaagaga
    14941 ccgaacacga ttcctgtatt cggtccaggg aaatggctct tgggagagag ccgtgcgcta
    15001 aaagttggca ttaatgcagg cttagttgcc ttgctcttta agaatagatg acgacgccag
    15061 gttttccagt ttgcgtgcaa aatggtcaat aaaaagcgtg gtggtcatca gcttaaatat
    15121 taaaaaccgc ccgttctggt gaaagaactg aggcggtttt ttattggaaa tcaaaaggct
    15181 attttaggta attaacagag tttttcagct cgctctataa acggtgccag actcattttt
    15241 tcgccgggat tgttaggatc atcaatctga atcaccgaaa tgggttgggc tttagtcttc
    15301 ccactggcaa cttccttttg tgcgatatcg tttaaaggat actgcacgag agtacttggg
    15361 ttaatgacat acaaagcatt acccggtcgg caagtcagca tcacctcttc gcgattaaac
    15421 gcccatttgt ctttacctac ttcaaaacgg ctgacggtaa tcacctgcgg tgccgccagc
    15481 gcagctgcag aactggtgag taacagaaac gccagaatac tttttttcat cattttcgta
    15541 atccatcaaa aaggttcgag agtcgcaagc aggctgatga tcaccagcac taccgcacct
    15601 atcgcccatt cgagtttagt catccagaca aaccagggag ccgcacgatc ttcatcctgc
    15661 ctcatgcgcg gtacaagaac ataacgatta gccagcgcga tgaccaccat aatcatcacc
    15721 aggatggctt tgagcaataa gagttggccc cagtgagtcg taagcgtggg gggaaatccg
    15781 gtaatcaaca atgcattaag cacgccgctt gccagtacgc cgatcaccgc aaaatgcccg
    15841 caccaggaaa aacgcatcag cgcctgaata gcctggtgtc gccaacggcc tttgatgagt
    15901 tgcatacacc agagcaccgg cagtaatccc ccaaaccacg ccgccgcaca gatcaggtga
    15961 attgcatgat tagtctgatg gattttcgcc gttacccctt cattcagcgt cgcatgaccg
    16021 actcccgcca gcagaataaa ttgcgcggta gtgagcataa atagcaaacg tggcatattc
    16081 tgcggttgca taagggcgac gatcaatgtt acgagggcga gaacaatttg ccataaccag
    16141 acgccaccaa actgcgtttg caaaacagcc agccaaatgt ttggcgaaaa tacatctgac
    16201 catcccgttc ccatcaaccc gccctgaacg gcgagcattg cagtggcact gatcaaactc
    16261 cagacggcag cgtgctgttg taaacgcaga aaacgcttcg tcagcaagcg acgaatcgtt
    16321 aagggggcca gccaggcacc gtacattgcg aagccaaaaa ccagcatcag cgaggtgaaa
    16381 tggataaatc gcagcgcgat ccaggtaaac gccagcatga tttatttcac gctaaaggtg
    16441 taatgtcctt tcgttttatg cccatccacc gaaacaacat gccagtcaac ggtgtaggtt
    16501 ccgggtttca gcgaatcggc cagtggaaca atcagttgct tttggtcctg ctcattacgc
    16561 ttcgccggta atgttttaat attttcgttt tttggccccg tgatttttgc gccactgaat
    16621 cctgtttcaa cgccttccga gaagtttaaa gtgattgcct gcggagctgc tgtcacttgt
    16681 gcatttgccg caggatactg atgcgttaaa tgcgcatgtg cccagacgga aggtgtcacc
    16741 aacgaagtgg tcaggattgc cagcgcgtag cgaagggagc gtgcagttga agccatatta
    16801 tctattcctt tttgtaataa cttttttaca gagcataacc ttgtctaatg tctgagtcga
    16861 ggatcatcaa ttccggcttg ccatcaaggc tcactcttag taacttttgc ccgcgaatga
    16921 tgaggagatt aagaatgctg aagaatctgg ctaaactgga tcaaacagaa atggataaag
    16981 tgaatgtcga tttggcggcg gcaggggtgg catttaaaga acgctacaat atgccagtga
    17041 tcgctgaagc ggttgaacgt gaacagcctg aacatttgcg cagctggttt cgcgagcggc
    17101 ttatcgccca ccgtttggct tcggtcaatc tgtcacgttt accttacgag cccaaactta
    17161 aataaaactt atacatagtt acactttctt acataatgcc tgctaaatta tgagtatttt
    17221 ctaaaccgcc cccataattt gcagtcattt tgaaaaggaa gtcattatgt cgttctggaa
    17281 agttgcagca gcgcaatatg agcctcgtaa aacctcactc accgaacagg tagctcatca
    17341 tctggagttt gtgagggccg ccgccaggca acaatgtcag ctgttagttt ttccatcgct
    17401 ttctttactg gggtgtgatt attcgcgacg tgcccttcct gccccacccg atctctcgct
    17461 attagacccg ctttgttatg ccgcaacgac ctggcgaatg accatcattg ccggccttcc
    17521 cgttgaatat aacgatcgct ttattcgtgg aattgcggta ttcgcaccgt ggcgaaaaac
    17581 gccagggatc taccatcaga gttatggtgc atgtctgggc cgtcgttcca gaaccatcac
    17641 ggtggtcgat gaacaaccgc aaggtatgga tatggacccg acctgttcac tgtttacgac
    17701 agggcaatgt ctgggagaac ccgacctgct ggcgtcggcc cgtcgcttac aatttttttc
    17761 acatcagtac tcgattgctg tgctgatggc aaatgcccgt ggtaacagtg cactatggga
    17821 tgaacatggt cgtctcatcg ttcgcgccga tcgtggttca ttattgttag tcggtcagcg
    17881 ttcatcacag ggttggcaag gcgatatcat tccattacgc taggcttttt cggcctggag
    17941 catgccatgt tgcgcattat cgatacagaa acctgcggtt tgcagggagg gatcgttgag
    18001 attgcctctg ttgatgtcat tgacggaaaa atcgtcaatc ccatgagcca cctggtgcgt
    18061 cccgatcgtc ctattagtcc acaggcgatg gcgattcatc gcatcaccga agccatggtc
    18121 gccgataaac cgtggattga agatgtgatc ccgcactatt acggtagtga atggtatgtc
    18181 gcgcataacg ccagctttga ccgccgcgta ctgcctgaaa tgcccggtga gtggatttgc
    18241 actatgaaac tggcccgtcg tttgtggcca gggatcaagt acagcaatat ggcgttatat
    18301 aaaacacgga agctcaatgt acagacgccg ccgggcctgc atcatcaccg cgcgttgtat
    18361 gactgttata tcaccgctgc gttgcttatc gatattatga acacctccgg ctggacggca
    18421 gaacagatgg ccgatatcac cggacgtcca tcgttgatga cgactttcac ctttggcaaa
    18481 taccgtggca aagcggtttc cgacgttgcc gaacgcgatc cgggctatct gcgctggtta
    18541 tttaataacc tggacagcat gagcccggag ctgcgtttaa cactgaaaca ttatctggaa
    18601 aatacttaat ccgcaggcgt agcgggtaat gttccctgcg ccagcgcgac cagaaaagca
    18661 tattccatcg ctacgccttc gtacgattta aagcgaccag atttaccgcc atggcctgag
    18721 tccatgtcgg tacagagcaa taaaagatgg ttatcggttt tcagctcgcg caatttagcg
    18781 acccattttg ccggttccca atattgcacc tgagaatcgt gtaaaccggt cgttaccagt
    18841 aaatgcggat aagcctgtgc ggtgacgttg tcatacgggc tgtagctttt catatactcg
    18901 taatattgcg gatcctgcgg attcccccac tcttcaaatt cgccggtggt aagaggaatt
    18961 gattcatcca gcattgttgt tacaacatca acaaacggca cctgggcgat aacgccgtgg
    19021 aacaattccg ggcgttgatt aatcgcaacg cccatcaaca taccccccgc actcccgccc
    19081 atcgcataac agagcgaagg agagccatag cccagtttta acaatgcatc gcaggcatca
    19141 agataatcat taaacgtatt tttcttcttc agaaatttac cgtcctcgta ccattgttgc
    19201 cccagctcac cgccgccacg aacatggaca atggcgtaga caaatccacg atccaacaaa
    19261 ctcaagcggc taaaactgaa atcggcatca atacttgcgc cgtaagaacc gtagccatac
    19321 accagcagcg ggttgtgtcc tttgcgaaaa tgtttgcgat gatagaccag cgaaaccgga
    19381 acttcgacgc catcacgggc gactatccac aggtgttcac tgcggtaatt cgccgcatca
    19441 aaaccaggaa cttccgtttg ttttaataca cgacgctcac cggtatccat atccagttca
    19501 aacaaagtat ctggtgtggt catggacgaa taaccataac gcaatcgcgc ggtttcaggt
    19561 tctggattgt aggcaatcca ggtcacatag gccggatcat caaaggcaat gccaatgact
    19621 tcccgggtct tgcggttaat ttgccgcaaa ctggttaacc cgcgctgacg ctcttcaacc
    19681 accagccagt cggtaaacag cgtaaaccct tccagcataa tgttatcacg tggcggaatt
    19741 aactcttccc actgttgctc atcacgcata cgggtacggt ataagccaaa gtttttaccg
    19801 tggcggttgg aacgcagata aaaccgatgc tggtagtgat caaggctgta ttcgtgatct
    19861 ttgcggcgcg gcagaaaaac aaacggctcg gcatcggcta tttccgcgtc cagcaggcga
    19921 acttcactgg tggtggcgct ggccagatga atgactacat agtgcttcga ggtcgtttta
    19981 tgcaggctga cgtaataggt atcgtctttt tcttcgtaga tcagtttatc ctgcgatgct
    20041 ggcgtaccga tggcgtgacg ccagacctga taaggaagca gcgtcaccgg atgcttgcga
    20101 acatagtaga aagtccagga atcatttgcc cagacaaagc tggggtcaac gttatccagc
    20161 agttccggat accagttacc tgtttccaga ttacgaaaac gaatgccgta ctggcgtcgg
    20221 gaaagaaaat cttctgccag tgccataatg gtgttatcgg gcgtaatcgc cattccgccc
    20281 atcgaataaa actcactatg agccgcgcgc ttgttggcat cgagcaatgt ttcccactca
    20341 tcccactctt cgctgaacgc cgattgacgc tggtagatag catattcaca gcctggttca
    20401 taaatatgcc gatagcggta gccatttttt atgtagggcg cagaaacttc tctttgcgga
    20461 atgcggtcga tgatttcctt taagatgcga tcctgcaagg cttgttgcga ggccatcacc
    20521 cgatgaccgt aactattttc ttgttgcaga tagtccagga cttccggctg agaacgcgta
    20581 tcgtcccgca gccagtagta attatcgatg cgcgtatcgc catgaagcgt catggcgtgg
    20641 ggaatgcggg cggcttttgg tagcatgtta ttgttctttc tggttgaaac atcttataag
    20701 ggtggcaaaa ctcaccgggg atgcaagcga aacagggtag tcattgctta gatgatgaca
    20761 ggtaatggcg cggatatcga ctgttatgca gacagagaaa atcagcctgt tcaaatggct
    20821 gtgcggttct ggataacccg aaatagtcaa cttcaggcta tccagagagc ggaattactc
    20881 cgccaaagta cgtttttgct gttcgagatc gcgttcaatg ccttcacgaa cgtcctgggg
    20941 gattttcagc gcatcaccca gtgcattcag gtaactgcgt tccataaaat ggtcaatatc
    21001 aatagccgcg cagctcagga aatagatttc cagcgcctct tcttcattgc ggaccccggt
    21061 agccaggcgt tgtggatcca gcggttgttc gattgcctgc tcaatgagta cacgcccctt
    21121 ctcttccaca ccggcttcac gcaattgcag gtcgattgcc gcacgttctt tggcatcaat
    21181 atgaccatca cttttagcgg caaagaccag cgcaaggatc aaacgttccg tacgctcatc
    21241 cagcggcgta ctttgcgcgc caaactgcgg ttcgtcctga tgcgccgcgc gaattttatc
    21301 tttgtattta ttccacagca ccgtacccgc taccgctccg ccgccaacca gtaacgcgtt
    21361 tgtgccatat ttcgtaagaa gtttacgtgc tgatttattt gcgaccagca gcccagccag
    21421 tccgcctaat gcgccaggca ctaaaagttt gcccaatccc tgatccgcag acgaggaggt
    21481 agaagaacgg ctttgcccaa gcagggattg cagttgattt aaccagttag ccatatttgc
    21541 tcctcaataa ccatttttag tgcctgtagc atagcggatg aggatgtcag aaaatgtata
    21601 tcgagacaaa agatgcgcaa ataagcgggg cggtaggccg ttattcaaag aaattcgcgc
    21661 caggtaaggt gttcactgta tataaggtga taagggtatt ttaggtgaaa gtagtttgac
    21721 attttgagtg gaactgtttg atgctgattg cccggttcga cgctactgcg tcttctcagg
    21781 cctacgattg tgttgcgttg tagggcggac aaggcacccg cgccgcatcc gccaacggtc
    21841 aatcgaatta tcagaagcca ttaacgccgc tgatattccg ccattcccgc tttgcagtcc
    21901 acgctgacct ggtaatgaat atcggcactt ttaccacgca cggttagcgg tactgaccat
    21961 ttatcatctt tcccctgaat gtcctgcaaa ctgacccatg ccacgggatc ggcctgaccg
    22021 acaatttttt gatcatcagc ccagcgcgcc acgcgatttt gttggtaatc acgttttacg
    22081 ctcgcggcaa ttccggcagc atccagacct tcacactttg ggaaagtgac cgacttgctg
    22141 gtttcattat tggcagcgaa aactgatgcg caggcagaaa ccaacaacag ccctaaaaac
    22201 gcccctcttt ttttcatgtt tttctccata gcacaatgat tcaggagaaa gcatggtaca
    22261 aattgtcagg agcgcaagtt gcttcaggcc gcgtgtgagg catcttcctc ttcggattca
    22321 gcaccgacga cctgtgggga cggtaatttg ccggttttca gaatggtgct cagcacatct
    22381 ttttgttctg ccagccatag cgaaagggct tcacgttgct cgtcttccat ttgcactgga
    22441 gaatttgtca gccaggtgtc aagcagatcg gccgtatcaa gcatcttgtc ataagcatcg
    22501 gcttcctttt tgctggtaaa cgacattttc tcttcgccct cacgaatgac tacgtattta
    22561 acttcaaccg ccatttgcag cctctcataa taactgtggt tttatacagt atatttcttt
    22621 tcagttggga aatcaacatc agcaataaag acacacgcaa acgttttcgt ttatactgcg
    22681 cgcggaatta atcaggggat attcgttatg acgttattag gcactgcgct gcgtccggca
    22741 gcaactcgcg tgatgttatt aggctccggt gaactgggta aagaagtggc aatcgagtgt
    22801 cagcgtctcg gcgtagaggt gattgccgtc gatcgctatg ccgacgcacc agccatgcat
    22861 gtcgcgcatc gctcccatgt cattaatatg cttgatggtg atgcattacg ccgtgtggtt
    22921 gaactggaaa aaccacatta tatcgtgccg gagatcgaag ctattgccac cgatatgctg
    22981 atccaacttg aagaggaagg actgaatgtt gtcccctgcg ctcgcgcaac gaaattaacg
    23041 atgaatcgcg agggtatccg tcgcctggcg gcagaagagc tgcagctgcc cacttccact
    23101 tatcgttttg ccgatagcga aaaccttttc cgcgaagcgg ttgccgccat tggctacccc
    23161 tgtattgtta aaccggtgat gagttcttcc ggtaaagggc agacgtttat tcgttctgca
    23221 gagcaacttg ctcatgcatg ggagtacgct cagcaaggtg gtcgcgccgg agcgggccgc
    23281 gtaattgttg aaggcgtcgt taagtttgac ttcgaaatta ccctgctaac cgtcagtgcg
    23341 gtggatggcg tccatttctg tgcaccggta ggtcatcgcc aggaagatgg cgactatcgt
    23401 gaatcctggc aaccacagca aatgagcccg cttgcccttg aacgtgcgca ggagattgcc
    23461 cgtaaagtgg tgctggcact tggcggttat gggctgtttg gtgtcgagct gtttgtctgt
    23521 ggtgatgagg tgattttcag tgaagtctcc cctcgcccgc atgacaccgg gatggtgacg
    23581 ctaatttctc aggatctctc ggagtttgcc ctgcatgtac gtgccttcct cggacttccg
    23641 gttggcggga tccgccagta tggtcctgct gcttctgccg ttatcctgcc gcaactgacc
    23701 agtcagaatg tcacgtttga taatgtgcag aatgccgtag gcgcagattt gcagattcgt
    23761 ttatttggta agccggaaat tgatggcagc cgtcgtctgg gggtagcact ggctactgca
    23821 gagagtgttg ttgacgccat tgaacgcgcg aagcacgccg ccggacaggt aaaagtacag
    23881 ggttaaaccc ggcaaaaaaa cgctacaaaa atgcccgatc ctcgatcggg cattttgact
    23941 tttacagctt agcgccttct acagcttcac gcgccagctt agtaatgcgg tcgtaatcgc
    24001 ccgcttccag cgcatctgcc ggaaccagcc aggaaccacc gatgcacagc acgcttttca
    24061 gcgccaggta gtcacggtag ttagccggag aaataccacc tgtcgggcag aaacggacct
    24121 gggagaacgg acccgcgatc gcctgcaggg ctttcacacc gccgttagct tcagccggga
    24181 agaatttgaa ctctttcaaa ccgtagtcca tacccagcat cagttcggaa acagtgctga
    24241 tccccggaat cagaggaata gtcccttcgg tagcagcttt cagcagcggc tcggtcagac
    24301 ccgggctaat tgcaaactgt gcacctgctt cagtgacttc tgccagctgc tgtggattca
    24361 gcaccgtacc ggcacccaca atcgcttcag gcacttcttt ggcgatagca cggatagcgt
    24421 caactgcgca ctcggtacgc agagtcactt ccagaacgcg caccccacca gcaaccaacg
    24481 cttttgctat cggcaccgcg tgttccagtt tttttaccac gataaccggt acaaccgggc
    24541 cggtggtcag gattgattct gcacttgttt tccagttttt catcagagtt ttctctcgcc
    24601 tgattacaaa tttgtcgtct taaaaagtga tacaggttgc gccctgttcg gcaccggaca
    24661 gtttttcacg caaggcgctg aataattcac gtcctgttcc cacgcgtgac gcgctcaggt
    24721 caggaatgtg cggttcgcga gcagccagtt ccgcttcgtc taccagcagc gtcagttcgc
    24781 ctgtctgtcc attcacacga atgatgtccc cgtcgcgcac ttttgccagc agcccgccat
    24841 cgtaggcctc tggtgttacg tggatagctg acggcacttt acctgaagcg ccggagagtc
    24901 gtccatcggt aactaacgca attttgaaac accggtccaa taatacacca agtggcggca
    24961 tgagtttatg taattctggc attccgttcg cttttggccc ctgatgacgg acaacaacga
    25021 cacaatcgcg gtccagtaaa cccgcttcaa aggccggcat aacgtcatgc tggctttcaa
    25081 aaacaaccgc tggcgcttca atcacctggt tctctaccgg cacggcagag gttttcataa
    25141 ccgctcgccc caggttaccg cttaacactt ttgtcccacc atgatgagag aaaggttgtt
    25201 cgaaggacgc gatcacattg ttgtcgagtg atttttctgc cccttcccgc cagtccagtt
    25261 caccattatt cagccatggt tcaagggtat aacgagacag accaaagcct gccaccgtat
    25321 tgacatcttc atgcagcagg cctgctttga gcagttcacg taccagaacc ggtacgccac
    25381 ctgccgcctg gaagtggtta atatcggccg gaccgttcgg gtagagacgt gccatcagcg
    25441 gtacaacatc agaaaggtca gagaagtcat cccagttaat ctgaataccg gctgcgcgcg
    25501 ccatcgccac taggtgcatg gtgtggttag tggaaccacc ggtcgccagc agtgcaacga
    25561 taccgttcac caccactttc tcatcgatca tcttaccgat cggcatccat tcattaccat
    25621 taccggtcat gcgcgtaacc tggcgcgcag ctgcagcggt caaagcatcg cgcagcggag
    25681 aatccggatg aacaaaagaa gagcctggca actgcatccc cataaactcc accaccatct
    25741 ggttggtgtt ggcagtaccg tagaaagtac atgttcccgg cgcatgataa gacgcggctt
    25801 ctgactccag taatgccatg cggtccactt taccttcggc ataaagctgg cgaatacgca
    25861 ctttttcttt atttggcaaa ccgcttgcca tcggtccaga cggcacaaac accgcaggca
    25921 aatgaccaaa cgacagggct gccatggtca gacctgggac aatcttgtcg cacacaccga
    25981 gaaacagagc accatcaaac atgttatggg acagccccac cgccgcagac atcgcaatca
    26041 cttcgcggct taacagcgac aattccattc catcctgccc ctgggtgaca ccatcacaca
    26101 tcgccggaac accgcccgca acctgaccaa ccgcattcgc ttcatgcagg gctttacgaa
    26161 tgatttctgg atagtgttca taaggctggt gagcggagag catgtcgtta taggaggtga
    26221 tgatggcgat attgttacgc aacatgcttt tcaaagaggc tttgtcttct ggctggcagg
    26281 cagcgaaacc gtgtgccagg ttaccgcatg ccaactgcga acgatgaacg gtcgaagttt
    26341 tcgcttgttc tatccgggcg aggtaagcag agcgagtctc gcgcgaacgt tcaatgattc
    26401 gatttgttac gcgtaacaat tgtgggttca taaaggctcc tgaaattgag ttgtcagagc
    26461 aggatgattc acaacgcgtt tcatacatgg aaattatgat tgaaacgcct gtaaccggag
    26521 ctcatagggc aaaaacgttt cagtcagtgt aataaaaaaa gcctcgtggg tgaatccgca
    26581 cgaggcctga aagtgtaaaa attgttctac aatctgtgca agatcatgtt accggtaaaa
    26641 taaccataaa ggatgagcgc agatattact caaactcatt ccaggaacga ccatcacggg
    26701 taatcatcgc cactgaggca acgggtcccc aggttccggc ctgatacggt ttcggcgcat
    26761 cattgtccat cgcccacgcc tcagtaatgg agtctaccca tttccaggct tcttccactt
    26821 cgtcgcgacg tacaaacagt gcctgaatac cacgcatggt ttccagcagc aaacgttcat
    26881 aggcatccgc cagatgcgtc tgattaaagg tttctgaata gctcagatcc agcttggtga
    26941 tttgcaggtt atgtttgtgg tcaaggccag gaactttatt cagtacctgg atatccacgc
    27001 cttcatcagg ttgcagacgg atagtcagtt tattttgcgg tagatcctgc cacgactctt
    27061 taaacagatt cagttcaggt gttttgaaat agaccacgac ttcagaacat ttggtcggca
    27121 gacgtttacc agtacgcagg tagaatggca caccggccca gcgccagtta tcaatgtcga
    27181 cgcggatcgc cacgaaggtt tctgtattgc tgctcttgtt cgcgccctct tcttccagat
    27241 agcccggcac ttttttgccc tgagcgaagc ccgcagtata ttgcccgcgt acggtttttt
    27301 cgcgcacatt ggagcggtcg atgcggcgca gagacttcag tactttcact ttttcatcgc
    27361 ggatgctgtc tgcgctcagg tcagacggcg gagacatcgc aatcatgcaa agaatttgca
    27421 gcaggtggtt ctggatcatg tcgcgcatct gaccggcttt atcaaaatag ccccagcgcc
    27481 cttcgatccc cacttcttct gccacggtaa tctcaacatg atcaatggtg cgattgtccc
    27541 agttgttcac aaacagagag ttagcaaaac gcagcgccaa caggttcagc actgtttctt
    27601 taccaagata gtggtcgata cggtaaacct ggcactcctc gaagtattcg ccaacctgat
    27661 cgttgatttc ctgcgaggtc gccagcgacg tccccagcgg tttttccatg actacgcgtg
    27721 ccggtttagc attcagtttt gcctcaccaa gccctttgca aattgcgcca aaagtgctgg
    27781 gcggcatggc aaagtagtta atggtgatac gatttttttg atccagcatc gcgccgagac
    27841 ggctgaatgc agcagtgtca ttgacatcaa gattacaaaa atccagacgt gcactcaggg
    27901 tgtcccataa accttcatca atggtttctt tcatgaaagt ttcgagcgcc tcgcggacaa
    27961 ctttggtata agccgcttta tcccagtcag cacgccctac gccgataatc cgggtgtccg
    28021 ggttgagctg accggctttt tccagttgat acagggaagg cagcaattta cgacgcgcaa
    28081 ggtcgccttt cgcgccgaaa atgaccaggt cacaggcctg ggctgtttgc gttaccgcca
    28141 tgtcattctc cttaagttaa ctaacccggt acttaagcca gggtatactt gtaattttct
    28201 tacggtgcac tgtactgctt ttacgagctt gcgaaaactg taaacgctta tccacccgtg
    28261 cgattacggt aaaagcgcgc aaagtgcggc aaaactgata aaaaaatcat cgttttccat
    28321 tgggtaaaaa tctgacactg atcatgttat gaaaaaaaat aacaactttt ttatctgctt
    28381 ttgtcattaa cacagcacac aggcgtaata tctgacaaaa ctgcatttcg atttctttca
    28441 gagcggaaat cgtcattacc cgtgagtctc tttacatcat gaatatgctg gaaaaaatcc
    28501 agtctcagct ggaacatttg agcaaatctg agcgcaaagt tgccgaggtc attctggctt
    28561 cgcccgataa cgcgatccat tcgagtattg ctgctctggc gctggaagcc aatgttagcg
    28621 aaccgacggt gaatcgtttc tgtcgcagca tggacacacg cggttttcct gattttaaac
    28681 ttcatctggc acaaagtctg gcgaatggca ctccctatgt taatcgcaac gtcaatgaag
    28741 atgacagcgt tgaatcatac acggggaaaa tatttgagtc cgcaatggca acgctcgatc
    28801 atgtccgtca ttcactggat aaatctgcca tcaaccgcgc cgtcgacttg ctcactcagg
    28861 caaaaaaaat cgcctttttc ggattaggtt cttcagccgc cgttgcccac gatgcgatga
    28921 ataagttctt tcgttttaat gttccggtgg tgtactccga tgatatcgtg ctgcaacgca
    28981 tgagttgtat gaattgtagc gacggagacg tggtggtgct gatttctcac acgggaagaa
    29041 caaaaaatct ggtcgagctg gcgcagctgg cacgcgaaaa cgacgctatg gtaattgccc
    29101 tcacctctgc gggtacccca ctcgcccggg aagcaacgct ggcaattacc ctcgacgtac
    29161 cggaagatac tgacatttat atgcccatgg tttctcgact tgcacagctg accgtgatag
    29221 atgtgctggc gacaggattt actttgcggc gcggtacaaa attcagagat aacttgaagc
    29281 gggtcaagga agcgctgaag gaatcgcgtt ttgataagca gttacttaat ttaagtgacg
    29341 atcgctaaaa acgactgtca ctgtcctaat cttatacgac atccgaatga gattaattta
    29401 tcgccatcac ggcgttattt cattcggatt tcatgttcaa gcaacacctg gttgtttcag
    29461 tcaacggagt attacatgtc cagaaggctt cgcagaacta aaatcgttac cacgttaggc
    29521 ccggcaacag atcgcgataa taaccttgaa aaagttatcg cggcgggtgc caacgttgta
    29581 cgtatgaatt tttctcacgg ctcgcctgaa gatcacaaaa tgcgcgcgga taaagttcgt
    29641 gagattgccg caaaactggg gcgtcatgtg gctattctgg gtgacctcca ggggcccaaa
    29701 atccgtgtat ccacctttaa agaaggcaaa gttttcctca atattgggga taaattcctg
    29761 ctcgacgcca acctgggtaa aggtgaaggc gacaaagaaa aagtcggtat cgactacaaa
    29821 ggcctgcctg ctgacgttgt gcctggtgac atcctgctgc tggacgatgg tcgcgtccag
    29881 ttaaaagtac tggaagtcca gggcatgaaa gtgttcaccg aagtgaccgt cggtggtccc
    29941 ctctccaaca ataaaggtat caacaaactt ggcggcggtt tgtcggctga agcgctgacc
    30001 gaaaaagaca aagcagacat taagactgcg gcgttgattg gcgtagatta cctggctgtc
    30061 tccttcccac gctgcggcga agatctgaac tatgcccgtc gtctggcacg cgatgcagga
    30121 tgtgatgcga aaattgttgc caaggttgaa cgtgcggaag ccgtttgcag ccaggaggca
    30181 atggatgaca tcatcctcgc ctctgacgtg gtaatggttg ctcgcggcga cctcggtgtg
    30241 gaaattggcg atccggaact ggtcggcatt cagaaagcgt tgatccgtcg tgcacgtcag
    30301 ctaaaccgcg cggtaatcac ggcgacccag atgatggaat caatgattac taacccgatg
    30361 ccgacgcgtg cagaagtcat ggacgtagca aacgccgttc tggatggtac tgacgctgtg
    30421 atgctgtcgg cagaaactgc tgctggtcaa tacccgtcag aaactgttgc agccatggcg
    30481 cgcgtttgcc tgggggcgga aaaaatcccg agcatcaacg tttctaaaca ccgtctggac
    30541 gttcagttcg acaatgtgga agaagccatt gccatgtcag caatgtacgc ggctaaccac
    30601 ctgaaaggcg ttacggcgat catcaccatg accgaatcgg gtcgtaccgc gctgatgacc
    30661 tcccgtatca gctctggtct gccaattttc gccatgtcac gacatgaacg tacgctgaac
    30721 ctgactgctc tctatcgcgg cgttacgccg gtgcactttg atagcgctaa tgacggcgta
    30781 gcagctgcca gcgaagcggt taatctgctg cgtgataaag gttacttgat gtctggtgac
    30841 ctggtgattg tcacccaggg cgacgtgatg agtaccgtgg gttctactaa taccacgcgt
    30901 attttaacgg tagagtaagt acgttgccgg atgcggcgaa aacgcctcat ccggcctaca
    30961 gttcaatgat aattcaacag atttcgaatc ttctgaagca aacttgaact tatcatcagt
    31021 cgaaggcctc tcctcgcgag aggcattttt tatttgatgg gataaagatc tttgcgctta
    31081 tacggttgga tttcgcccgg tttgcgagtt ttcagcaatt ttaatatcca ggtgtattgt
    31141 tctggtcgcg gaccaacaaa aatctcgact tcttcattca tccgccgcgc aatcgtatga
    31201 tcatccgcct ctaacagatc atccatcggt gggcgcacct gaatcgtcag acgatgcgtc
    31261 ttgccatcat aaatcggaaa cagcggtaca acgcgcgcac ggcacacttt catcaaacga
    31321 ccaatcgcgg gcaacgtcgc tttataggtg gcaaagaaat caacaaattc gctgtgttct
    31381 gggccatgat cctgatcggg taaataatat ccccagtaac cctgacgtac cgactggatg
    31441 aatggtttaa taccatcatt tctcgcatgc agacgaccac caaagcgacg gcgcaccgtg
    31501 ttccagacat aatcaaaaac cgggttgccc tgattatgga acatcgctgc cattttctgc
    31561 ccttgcgagg ccatcagcat ggcaggaata tcgacggccc aaccgtgcgg caccagaaaa
    31621 atcactttct cgttattacg ccgcatctct tcgatgatct ccagcccttg ccagtcaacg
    31681 cgcggctgaa ttttctccgg cccgcgtatt gccaactcag ccatcattac catcgcttgc
    31741 ggcgcggtgg caaacatctc atctacaatc gcttcgcgtt cagcttcact acgttctgga
    31801 aagcagagcg acagattgat taacgcacga cggcgtgagc tttttcccag tcgtccggca
    31861 aaacgtccca gccgtgccag aatgggatca cggaactttg gcggcgttaa agcgataccc
    31921 gccatcgctg ctacgcccag ccatgctccc cagtagcgcg ggtggcgaaa ggatttatca
    31981 aactcaggaa tgtattcgct attatttttt ttcgtttcca tgcttttcca gtttcggata
    32041 aggcaaaaat caatctggtg atagtgtagc ggcgcaactt gccccgcacc aaataaaaaa
    32101 gccggtactg actgcgtacc ggctgcgaat ggatgttaat taatcaaacc gtagctgcgg
    32161 cacaatctct ttggcctgtg ccaggaattc gcgacgatcg gagccggtca gcccttcggt
    32221 acgcggcagt tttgccgtca gcgggtttac ggcctgctgg tttatccata cttcatagtg
    32281 cagatgcggc ccggttgaac gtccggtatt accggaaagc gcgatacggt cgccacgttt
    32341 caccttctgt cccggtttca ccaggatctt gcgcaagtgc atataacgcg tggtgtagct
    32401 gcgaccatga cgaatagcca cataataacc tgctgcgcca ctacgtttgg caaccaccac
    32461 ttcaccgtca cccactgaaa gcactggcgt accttgtggc atggcaaaat caacacctct
    32521 gtgtggcgca acgcgaccgg tcaccggatt agtacgacgc gggttaaagt tagatgagat
    32581 acggaactgt ttcgccgtcg ggaatcgcaa gaatcctttc gccagaccag taccgttacg
    32641 atcatagaat ttgccatctt cagcgcggat tgcataataa tctttacctt ctgaacgcaa
    32701 acgtacgccc agcagctggc tttgctcacg tttaccatca agcatttctc gtgacattaa
    32761 caccgcaaat tcatcgcctt ttttcagttt gcggaaatcc atttgccact gcatggcttt
    32821 aatcactgcg ctcacttcgg cgctggttaa accggcgttt ctggcgctgg caacaaagct
    32881 tcccccgacg gtacctttca gcagattgtt gacccactct ccttgctgca tttcgctggt
    32941 cattttaaaa ccgttagcgg cagtacggtc ataggttcgg gtttcacgac gagacacttc
    33001 ccaggtgagg cgctgcagtt cgccgtccgc ggttaatgtc caggagagtt gttgaccgat
    33061 tttcaggtta cgcaattctt tgtcggcagc agccagttgg gtgatatcac ccatatcaat
    33121 accatactga ttgagaatgc tgcttagcgt atcgccagtg gaaacaacat attcatgcac
    33181 gcccgcttca ccggcgattt tgtcatccag ttcgtcctgg ggaatggctt catcttcttg
    33241 tgcagcttga tcaatcggct cactggcttc aggtaagagc gaacgaattt cgttctgttc
    33301 cagctcaatg gttttgacaa ttggcgtggc atcacggtga taaacatagg gccgccagac
    33361 agcgacggcc agagtaagaa cggtgagcga ccccaacata acgcggtgtg gtcgcggtaa
    33421 attattaaac gccagggcga cagagcgggc tatctgttgc acgtaatcac ttcctcatta
    33481 atctcctttc aggcagctcg catactggtt ggctaattga ttcaggaatt ctgaatagct
    33541 tgttttaccc agtttgatat tcgtccccag gggatccaac gttcccatac gaacggatgt
    33601 ccctcgtgcg acgctctcaa cgaccgctgg cctgaactgt ggctcagcaa aaacgcaggt
    33661 tgctttttgc tcaaccaact gtgttcttat ttcatgtaaa cgctgcgcgc caggttgaat
    33721 ctcagggtta acggtaaaat gaccaagcgg tgtcagtccg aactgttttt cgaagtagcc
    33781 gtaagcatcg tgaaaaacga aataaccttt ccccttgagc ggcgcgagct cgttaccaac
    33841 ctgcgtttcg gttgaggcta attgtgcctc aaaatccttc aggttggcgt caagtttggc
    33901 tcgactttgc ggcataagtt ccactaattt tccatggatt gcaaccgctg tagcccgcgc
    33961 tatctctggg gaaagccaaa gatgcatgtt gaaatcgccg tgatggtgat cttcgtcact
    34021 tttttccgcg tgatcgtgat catcatcatc gtcgtgaata cttttcatca gcagcggttt
    34081 cacatcttct agctgcgcaa tcgttacctg tttcgcttct ggtaatttac ttaccggttt
    34141 ttgcatgaac gcttccatct ccgggccaac ccaaacgact aagtccgcgt tctgtaagcg
    34201 ttttacatcc gatggacgca gtgaataatc atgttctgaa gcgccgtcag gcagtaaaac
    34261 ctgcgtttct gttaccccat cagcaatggc agaagcgatg aacccaacgg gtttaagcga
    34321 agcgacaacg gcggcatctg cggcctgtgt tgcaccgccc cagagagcgg cggataatgc
    34381 tgcgaaaaga agcgtttttt tatgtaacat aatgcgacca atcatcgtaa tgaatatgag
    34441 aagtgtgata ttataacatt tcatgactac tgcaagacta aaattaacat gacaagtctg
    34501 gtttccctgg aaaatgtctc ggtttctttt ggccaacgcc gcgtcctctc tgatgtgtcg
    34561 ctggaactta aacctggaaa aattttgact ttacttgggc caaacggcgc aggtaagtcg
    34621 acactggtac gggtagtgct cggactggta acacccgatg aaggggttat caagcgcaac
    34681 ggaaaactgc gcatcggcta tgtaccgcag aagctgtatc tcgacaccac gttgccactg
    34741 accgtaaacc gttttttacg cttacgccct ggcacacata aagaagatat tttgcctgca
    34801 ctgaaacgtg tccaggccgg gcatctgatt aacgcaccga tgcaaaagct ctcgggtggc
    34861 gaaacgcagc gtgtactgtt agcgcgagca ttgttaaatc gaccgcaatt attagtgctg
    34921 gatgaaccca ctcagggcgt ggatgtgaat ggtcaggtgg cgttatatga ccttattgac
    34981 caactgcgtc gtgaactgga ttgtggcgtt ttaatggtat ctcacgatct gcatctggta
    35041 atggcaaaaa ccgatgaagt gctttgcctg aatcaccaca tttgttgttc cggcacaccg
    35101 gaagttgttt ccctgcatcc ggagtttatt tctatgtttg gtcctcgtgg tgctgaacaa
    35161 ctgggtatct atcgccatca tcataatcat cgtcacgatt tacagggacg aattgttttg
    35221 cgtcggggaa atgatcgctc atgattgaat tattatttcc cggttggtta gccgggatca
    35281 tgctcgcctg tgccgcgggt ccgctgggtt cgtttgtagt ctggcgtcgt atgtcttatt
    35341 tcggtgatac gctggctcat gcctcattac ttggcgtcgc gtttggtttg ttgctggacg
    35401 tgaatccatt ctatgcggtg attgcagtta cgctgctgct ggcgggcggt ctggtatggc
    35461 tcgagaagcg tccacagctg gcgatcgaca cgttattagg gattatggcg cacagtgccc
    35521 tgtcgctggg cctggtggtc gttagtctga tgtctaatat tcgtgttgat ttgatggctt
    35581 acctgttcgg tgatttactg gcagtgacgc cagaagatct catctctatt gcgattggcg
    35641 tggtcatcgt ggtggctatt ttgttctggc aatggcgcaa tttgctgtcg atgacgatta
    35701 gcccggatct ggcgtttgtt gatggtgtga aattacagcg cgtgaaattg ttgttgatgc
    35761 tggtgacggc attgacgatt ggtgtagcga tgaaattcgt cggcgcgttg attattactt
    35821 cactgctgat tattcctgct gctactgcac gtcgctttgc ccgcacgccg gaacagatgg
    35881 ctggtgtcgc tgttttggtg gggatggtgg cagtgactgg cggtttaacc ttttccgcat
    35941 tttacgatac acctgcaggc ccgtcggtgg tgctatgcgc ggcactgtta tttattatca
    36001 gtatgatgaa aaaacaggcc agctaatctt tcgctgaaca tatttgtcgg atgcggcgcg
    36061 agcgccttat ccgaccttgt attatccctc cagtgcagag aaaatcggtc agttttctct
    36121 gcctgcagtc cgcatgcctt atccgaccta cggttcgcta tctctggtag gcctgataag
    36181 acgaacatga actgaacccc aaaacctgga cagtttcagt taaccggctt tttgatactc
    36241 agtccggtat tctaccgggc tgagtccccc aagactgagt tttattcttt tttgattcca
    36301 gtaagttatg tattcgttaa cggcattttc cagctcttct acatttctgt agtcccggcg
    36361 atagtacatt tcttctttca ggtgaccaaa gaagttctcc attaccgcat tatcaaggca
    36421 gttgcccttg cgcgacatgc tctgcactaa ccctctgtcc gccagggcgg actgataact
    36481 ttttatccgg taatgccatc cttgatcact gtggagcagc ggtttctc
//
LOCUS       DACADF010000031        35615 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.031, whole genome shotgun sequence.
ACCESSION   DACADF010000031 DACADF010000000
VERSION     DACADF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.031"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            18..569
                     /locus_tag="ISB30_001494"
     CDS             18..569
                     /locus_tag="ISB30_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAP1989140.1"
                     /translation="MQNIAVVYYSGYGHTKLVAEKVAQGAHADLISIDNEGNITEQEW
                     EKLDAADAIIFGAPTYMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDK
                     QVTLIYLQTLASQHGGLWVSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGAEQI
                     PTGDLDTAVKYGERVATITARLK"
     gene            complement(740..1732)
                     /gene="rpoS"
                     /locus_tag="ISB30_001495"
     CDS             complement(740..1732)
                     /gene="rpoS"
                     /locus_tag="ISB30_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAP1989141.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(1795..2934)
                     /gene="nlpD"
                     /locus_tag="ISB30_001496"
     CDS             complement(1795..2934)
                     /gene="nlpD"
                     /locus_tag="ISB30_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAP1989142.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTVPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(3074..3700)
                     /gene="pcm"
                     /locus_tag="ISB30_001497"
     CDS             complement(3074..3700)
                     /gene="pcm"
                     /locus_tag="ISB30_001497"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAP1989143.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(3694..4455)
                     /gene="surE"
                     /locus_tag="ISB30_001498"
     CDS             complement(3694..4455)
                     /gene="surE"
                     /locus_tag="ISB30_001498"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAP1989144.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(4436..5485)
                     /gene="truD"
                     /locus_tag="ISB30_001499"
     CDS             complement(4436..5485)
                     /gene="truD"
                     /locus_tag="ISB30_001499"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAP1989145.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALLGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(5482..5961)
                     /gene="ispF"
                     /locus_tag="ISB30_001500"
     CDS             complement(5482..5961)
                     /gene="ispF"
                     /locus_tag="ISB30_001500"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAP1989146.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(5961..6671)
                     /gene="ispD"
                     /locus_tag="ISB30_001501"
     CDS             complement(5961..6671)
                     /gene="ispD"
                     /locus_tag="ISB30_001501"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAP1989147.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(6690..7001)
                     /gene="ftsB"
                     /locus_tag="ISB30_001502"
     CDS             complement(6690..7001)
                     /gene="ftsB"
                     /locus_tag="ISB30_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAP1989148.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(7194..7517)
                     /locus_tag="ISB30_001503"
     CDS             complement(7194..7517)
                     /locus_tag="ISB30_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAP1989149.1"
                     /translation="MRNSHNITLTNNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(7566..8171)
                     /gene="cysC"
                     /locus_tag="ISB30_001504"
     CDS             complement(7566..8171)
                     /gene="cysC"
                     /locus_tag="ISB30_001504"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAP1989150.1"
                     /translation="MALHDENVVWHSHPVTLQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(8171..9598)
                     /gene="cysN"
                     /locus_tag="ISB30_001505"
     CDS             complement(8171..9598)
                     /gene="cysN"
                     /locus_tag="ISB30_001505"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAP1989151.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEVLPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(9600..10508)
                     /gene="cysD"
                     /locus_tag="ISB30_001506"
     CDS             complement(9600..10508)
                     /gene="cysD"
                     /locus_tag="ISB30_001506"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAP1989152.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10760..11797
                     /gene="iap"
                     /locus_tag="ISB30_001507"
     CDS             10760..11797
                     /gene="iap"
                     /locus_tag="ISB30_001507"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAP1989153.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKTPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(11923..12075)
                     /locus_tag="ISB30_001508"
     CDS             complement(11923..12075)
                     /locus_tag="ISB30_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP1989154.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(12341..13075)
                     /gene="cysH"
                     /locus_tag="ISB30_001509"
     CDS             complement(12341..13075)
                     /gene="cysH"
                     /locus_tag="ISB30_001509"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAP1989155.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(13149..14861)
                     /gene="cysI"
                     /locus_tag="ISB30_001510"
     CDS             complement(13149..14861)
                     /gene="cysI"
                     /locus_tag="ISB30_001510"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAP1989156.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(14861..16660)
                     /gene="cysJ"
                     /locus_tag="ISB30_001511"
     CDS             complement(14861..16660)
                     /gene="cysJ"
                     /locus_tag="ISB30_001511"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAP1989157.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGTVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSDLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            16976..17341
                     /gene="queD"
                     /locus_tag="ISB30_001512"
     CDS             16976..17341
                     /gene="queD"
                     /locus_tag="ISB30_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAP1989158.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            17419..18690
                     /locus_tag="ISB30_001513"
     CDS             17419..18690
                     /locus_tag="ISB30_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP1989159.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILHARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSSVPASELLTRFKTHPAVRPLIKNTELLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLLWRHLRRHGLWHLTGDVIRSLRCL"
     gene            18681..18941
                     /locus_tag="ISB30_001514"
     CDS             18681..18941
                     /locus_tag="ISB30_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAP1989160.1"
                     /translation="MSVARNLWRAADAPHIVPTDSVERQTAQRLINACPAGLFSLTPE
                     GGLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            18958..19533
                     /locus_tag="ISB30_001515"
     CDS             18958..19533
                     /locus_tag="ISB30_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAP1989161.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(19681..20541)
                     /locus_tag="ISB30_001516"
     CDS             complement(19681..20541)
                     /locus_tag="ISB30_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAP1989162.1"
                     /translation="MNIVIVTINQDHAAIASWLAAQDFSGCTLAHWQIEPQPMVAEQV
                     LDALVEQWQRTPADVVLFPPGAFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTDKRPLCLSLARQAGADKNATLPSGMQQLNIVPGVLPDWLVSIENLKNV
                     IRDPLAEARRVLVVGQGGEADNQEIAMLAEKMGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(20538..21317)
                     /locus_tag="ISB30_001517"
     CDS             complement(20538..21317)
                     /locus_tag="ISB30_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAP1989163.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLVWPCFTQVERFTLDAPFI
                     TLEQRTEHGVRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAAIIRKTVAVELPA
                     MQCLQLARAEQRRGATLIGGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(21295..22704)
                     /locus_tag="ISB30_001518"
     CDS             complement(21295..22704)
                     /locus_tag="ISB30_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1989164.1"
                     /translation="MTVRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(22726..24180)
                     /locus_tag="ISB30_001519"
     CDS             complement(22726..24180)
                     /locus_tag="ISB30_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP1989165.1"
                     /translation="MSLSRVAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKKVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(24250..25035)
                     /locus_tag="ISB30_001520"
     CDS             complement(24250..25035)
                     /locus_tag="ISB30_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1989166.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            25354..26631
                     /locus_tag="ISB30_001521"
     CDS             25354..26631
                     /locus_tag="ISB30_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1989167.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIIFYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVVQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALGMVIIFTFLLFQKIRTADSAPAMASSK"
     gene            26658..28136
                     /locus_tag="ISB30_001522"
     CDS             26658..28136
                     /locus_tag="ISB30_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1989168.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFASHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     ATVMEKFNIPRQMLFDVQMPGTILGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYTTAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            28301..29221
                     /locus_tag="ISB30_001523"
     CDS             28301..29221
                     /locus_tag="ISB30_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HAP1989169.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDDLPAFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISDESQVQQGAYSLVQRYPLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IAQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            29389..30906
                     /locus_tag="ISB30_001524"
     CDS             29389..30906
                     /locus_tag="ISB30_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAP1989170.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNETFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            30967..32337
                     /locus_tag="ISB30_001525"
     CDS             30967..32337
                     /locus_tag="ISB30_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HAP1989171.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLK
                     YKTDEE"
     gene            32337..33746
                     /locus_tag="ISB30_001526"
     CDS             32337..33746
                     /locus_tag="ISB30_001526"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP1989172.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDLFALDGEYILLCCPQGMAREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            33772..34779
                     /locus_tag="ISB30_001527"
     CDS             33772..34779
                     /locus_tag="ISB30_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP1989173.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(34864..35535)
                     /gene="queE"
                     /locus_tag="ISB30_001528"
     CDS             complement(34864..35535)
                     /gene="queE"
                     /locus_tag="ISB30_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAP1989174.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT         8619 a         9250 c         8846 g         8900 t
ORIGIN      
        1 gtaaaaagga tatttatatg caaaacatag ctgtggttta ttattcagga tatggtcaca
       61 ctaaactggt tgctgaaaaa gtggcacaag gcgcccatgc agatttaatt tcgattgata
      121 acgaaggaaa tatcactgag caggagtggg aaaaactgga cgcagccgat gccattatct
      181 tcggtgctcc cacctatatg ggcggtgcgc catggcaatt caagaaattt gctgatgcca
      241 gttccaaagc gtggtttaca cgtaaatggc aggataaagt cttcgggggt ttcaccaaca
      301 gcgccagcct gaatggcgat aaacaagtta cgctgatcta tctgcaaacg ctggcttcac
      361 agcatggcgg tctgtgggtg agtttaggtc aggctcctgc caatgtgctt gcgtcaacca
      421 gggaagatgt aaacaatctg ggcggttcgg tcggtttgct ggtgcaatct ccagccgatg
      481 cgggtgcaga gcaaatccct acgggcgatc tggatacagc cgtgaaatat ggtgaacgcg
      541 tggcgacaat tactgcgcgc ttaaaatgat gattgccgaa tgtgatgctg acgcgtctat
      601 ccggtcttct acatctaaaa agtgccgtcc cctggtcagt cagataaagt tttaacgctg
      661 tatccgataa cagttcaaca tgtaccaaaa gaaaaaaagc cagcctcgct tgagactggc
      721 ctttctgaca gatgcttact tattcgcgga acagcgcttc gatattcagc ccctgcgttt
      781 gcaggatttc gcgcaaacgg cgcaggcctt caacctgaat ctggcgaaca cgttcacggg
      841 tgaggccaat ttcacgacct acatcttcca gtgttgccgc ttcgtacccc agcaaaccga
      901 atcgacgtgc cagcacttca cgctgtttgg cgttcagctc gaacagccat ttgacgatgc
      961 tctgcttcat atcgtcatct tgcgtggtat cttccggacc attctctttt tcatcggcca
     1021 ggatgtccag caatgctttt tcggaatcac cacccagcgg ggtgtctacc gaggtaatgc
     1081 gctcgttaag acgaagcata cggctgacgt catcaactgg cttatccagt tgctctgcga
     1141 tctcttccgc acttggttcg tggtccagct tatgggacaa ctcacgtgcg gttcgcaggt
     1201 aaacgttcag ctcctttacg atgtgaatcg gcaaacgaat agtacgggtt tggttcataa
     1261 tcgcccgttc gatcgtctgg cgaatccacc aggttgcgta tgttgagaag cggaaaccac
     1321 gttccgggtc aaacttctct accgcgcgga tcagccccag gttgccctct tcgatcaggt
     1381 ccagcaacgc cagaccacga ttgccataac ggcgggcaat ttttaccacc agacgcaagt
     1441 tactctcgat catccggcgg cgagaggcga catctccacg cagtgcgcga cgcgcaaaat
     1501 aaacttcttc ttcggccgtt aacagtggtg aataaccaat ctcaccaagg taaagctgag
     1561 tcgcgtccag cacacgctgt gtggctccct gcgataacag ttcctcttcg gccaaatcgt
     1621 tatcactggg ttcctcttct actaaggcct tttcgtcaaa aacctcaact ccgttctcat
     1681 caaattccgc atcttcattt aaatcatgaa ctttcagcgt attctgactc ataaggtggc
     1741 tcctacccgt gatcccttga cggaacattc aagcaaaagc ctggttccgc cgatttatcg
     1801 ctgcggcaaa taacgcagcg ggtttacgga tttccccttg taacgaattt caaaatgcaa
     1861 gcgtgttgaa ctggttccgg tgctacccat ggttgctatt ttttgccccg ccttcacttc
     1921 ttgttgttcc cggaccagca ttgtgtcgtt atgggcgtag gcactcaggt aatcatcatt
     1981 atgtttgatg ataatcagat taccgtagcc gcgcagcgcg ttaccggcat aaacaacgcg
     2041 gccatctgcg gtcgcgataa ttgcctgtcc tttgctgcct gcgatatcaa tccccttgtt
     2101 gcccccctca gaagcgccaa aggtttcgat cactttgccc tcagtcggcc agcgccaggt
     2161 ggagataggc gtactggttg atgtactgct gacagtcggc tcggttgtgc ttgctgttgg
     2221 taccgttaca ggcactgtga ccgtggtcgc agttggcttg ttgttcggca acattttgtt
     2281 agcactctgt tcacccgaag actcagaata cgtaattgtc ggttgcgacg caacagcaac
     2341 ggtggaattt tgtgcaggct tgatcacaac tccttgctct gctgcgtcgg cctgggtaat
     2401 ggcatttccg cctgtgattg gcgtaccgga agcattaccc acctgtaagg tctgaccgac
     2461 gttcagcgcg tatggtgcct gaatattgtt gcgctgagca aggtcacgga aatcgttgcc
     2521 agtaatccag gcgatataga aaagcgtgtc gccttttttc acggtgtatg tactgccgct
     2581 ataactgcct ttcggaatgt tcccatactg acggttgtag acgattcgtc cgttttccat
     2641 ctgtaccggc tgctgagcta ctggctgcac aggctggatt tgcggttgtt gagtagcctg
     2701 aatttgtggc tgctgtaccg gctgaatttg cggttgctgc gctgtagacg tcgtccccat
     2761 tttcggcggc ggcgtaatca acataccaga attggtattt gcgggcgcat tgccattaac
     2821 ggagctgacc ggtgccggtg gatttgaagt gtcagaacag cctgccagcc atagcgaaac
     2881 cagtgacaat gccgcaatgc ggcgaacggt gaattttggg cttcccgcgc tcatttatcc
     2941 cccaggaaaa attggttaat aaccagtgac ataattaccg tgcaaggcac catactgaac
     3001 actggaaaag atgttcacga tacgctgact tgcggcaaaa taaccaggaa aaatccaggt
     3061 atttcctcac gttttaagcc agctcaccct tcactaaagg gacaaagcgc acggcctcca
     3121 cggtatcgat aataaattcg cctccccgac gacgcacccg tttcaaatac tggtgctcct
     3181 cccctacggg taagacgaga atcccgcctt cgtccagctg cgtcattagc gcagttggaa
     3241 tttcaggcgg tgccgccgta acaatgatag cgtcaaacgg cgcgcgtgcc tgccaacctt
     3301 gccatccatc gccatgacgg gttgaaacat tatgtaaatc aagatttttc aggcggcgac
     3361 gcgcctgcca ctgcaagcct ttaatccgtt caaccgagca aacatgctgg acaagatgcg
     3421 ccaggattgc agtttgatat cccgaaccag tgccaatttc cagcacccgc gactgcggcg
     3481 ttagctcgag taattcggtc attcgcgcca ccatatacgg ctgcgaaatt gtctgcccct
     3541 gacctatcgg caaggcgata ttgtcccagg ctttttgttc aaacgcttca tcaacgaatt
     3601 tttcacgcgg cacggcggca agtgcattca gcacctgctc atcctgaata ccttgcgcac
     3661 gtaattgatc cagaagtgct tgtacgcgtc tgcttaccat tgcgtgccaa ctcccacgct
     3721 gtttaaccag tctgaaacca catcttgcgc gctatgcgca gttaaatcca catgcagcgg
     3781 cgtgatggaa acatagccct catctaccgc agcaaaatcg gtccccggac cggcatcaca
     3841 tttaccgccc ggcgggccaa tccagtacag cgtattgccg cgcggatctt gctgcgggat
     3901 cacctgatct gccggatgtc gtgtaccgca gcgcgtcacg cgaatacctt tgatttgatc
     3961 caatggtaaa tccggaacgt taatattaag aatacgcccg gtgcgcagcg gctctttaca
     4021 caatgcgcgc aaaattgagc aggttaccgc cgcggcagtg tcgtaatgtt tatgcccgtc
     4081 aagcgagacg gcaagcgccg gaaaacctaa atgacggcct tccatcgcgg ccgctaccgt
     4141 accggaataa ataacatcat cccccagatt cggcccggcg ttaattccgg acacaacaat
     4201 gtccgggcgc gggcgcatca gagcatttac gccaagatag acgcaatcgg tcggggttcc
     4261 catttgcaca gcaatatcac cattttcaaa ggtaaacgtg cgcagggaag attccagtgt
     4321 cagagaatta gaagcgccgc tgcggttacg atcgggggcg accacctgaa cgtcagcaaa
     4381 ctcacgcaag gctttcgcca gcgtttgtat accgggtgca tgtaccccgt catcattact
     4441 cagcaatatg cgcataatca cctgttgtgt tgataagttc cctgacaacg ctggttgcaa
     4501 aacttcccgc cggaagccag aaacggattt ctacggtgac gtcatcccac cagttccagc
     4561 ttaattgttg cggatacagc agcatcgctc tgcgcgcggc ttcaactttt tcacgcacca
     4621 gtaaagtttg taattcagtt tcttcggcga cagctgcttg ttcgaatgcc agcgcttcac
     4681 gctgagttcc ccattcgcca ctgcccagca atgcggcggt tatcattagc tctttatcgt
     4741 tgacgcgacg ctgtaattcc gccagttctt cggtggttgc aacaaaccag ctaccacgtc
     4801 cggctaattg tagcgcatcg ccgtcaacaa cttgattaac gtctgctttt ttgaggcgct
     4861 cagcaacaat ctgattaaac aacgcactgc gggctgccga caaccaaaaa ctccgtttat
     4921 tgcgatcgcg caccggagta ttggtttgcg cccagcgcag cgcgccctgc aagttgctac
     4981 cgccaatccc aaaacgttgg gcaccgaagt agttcggtac accttttacg caaatatcga
     5041 tcagacgttg ttcaacgtca tcgcgattgc tcacttcgcg cagaaccagg gtaaaggcgt
     5101 tacctttcag cgcgcctaaa cgcagcttgc gcttgtgccg cgcatactcc agcacctggc
     5161 agccttccag ttgaaaggcg ctcagatcgg gcatctcttt gcccggcacg cgagcgcata
     5221 accactgttc cgtaacagca tgtttatctt tttgcccagc gaagctgact tcacgggcat
     5281 gaattttcag gaatttcgcc agtgcatccg ccacaaaacg ggtattgcag ccatttttga
     5341 ggattctaac cagaatatgc tcaccttcac catcaggctc aaagcccaaa tcttccacca
     5401 ccacaaagtc ttccggattg gctttcagca gcccggtgcc ttgcggttta ccgtggaggt
     5461 aagtgagatt atcaaactca atcattttgt tgccttaatg agtagcgcca ccgcttcaca
     5521 ggcaatccct tccccacgtc cggtaaatcc cagtttttcc gtagtagtgg ctttcacgtt
     5581 aacatcatcc atatggcagc cgagatcttc ggcaataaac acgcgcattt gtggaatgtg
     5641 cggcaacatc ttcggtgcct gagcgatgat agtgacatcg acgttgccga gggcataacc
     5701 tttcgcctga atacgacgcc aggcttcgcg caacagctcg cggctgtcgg cacctttaaa
     5761 tgccggatcg gtatccggga acagcttgcc tatatctccc agcgccgccg cgccaagcaa
     5821 tgcatcggtc aacgcatgca gcgccacgtc gccatcagaa tgcgccagca gccctttttc
     5881 gtaaggaatg cgtacgccac caatgataat tgggccttca ccgccaaagg cgtgtacgtc
     5941 aaaaccgtgt ccaattcgca ttatgtattc tcctgatgga tggttcgggt gaggtaaaat
     6001 tcggccagcg ccaaatcctc cgggcgcgtt accttgatgt tatccgcgcg gccttcaaca
     6061 agttgcggat gaaaaccgca atattccagc gccgaggcct catcggtaat ggtagcgcct
     6121 tcatttagag cgcgcgtcag gcagtcatgt aacagctcac gagggaaaaa ttgcggcgtc
     6181 agcgcgtgcc ataagccgtt gcgatcaacg gtatgagcaa tggcattttt gcccggttcg
     6241 gcacgtttca tcgtatcgcg caccggcgca gcgaggatcc ctcccgtgcg gctggtttcg
     6301 ctcaacgcca acaatcgcgc gaggtcatcc tgatgcagac aaggacgagc ggcgtcatgc
     6361 accaataccc actgcgcgtc gccagcggct ttcagacctg ccagcacgga atcggcacgc
     6421 tcttcaccac catcaacaac cgtgattcgt ggatgattcg ccagaggaag ttgtgcaaag
     6481 cggctatcgc caggacttat ggcaatgacg acacgtttca cccggggatg cgccagcagc
     6541 gcatgcaccg agtgttcaag aatggtttga ttaccgattg agagatattg cttaggacat
     6601 tccgtttgca ttcggcggcc aaatccggcc gccggaacca cggcgcaaac atccaaatga
     6661 gtggttgcca tgttaattcc cgggctgatt tatcgattgt tttgccccgc agactgtgcg
     6721 cgctttgacg cgtcaggcac cagacgataa aacgtttcgc ccggcctggt catgctgagt
     6781 tcattacgcg cacgctcttc gagcgcctcc tggccgccat tgagatcgtc aatttcggca
     6841 aaaagctgat cgtttcgcgc tttaagtttc gcgtttgtag cttgctgtgc cgccacatca
     6901 ttattgacgc gagtatagtc atgtataccg ttcttaccga accacagcga atactgtagc
     6961 cagaccagaa tagccagcaa cagcagcgtt agtttaccca tcctgccccc tgaaaaacgg
     7021 catcttcatc ccatgcatcc gaagacgact ctacatcctc tgttggggat accgcgacaa
     7081 cgcgggcaaa tgtaccacat ttgtccattg ttacgtatac ccagggcgtg cagaaaataa
     7141 tctcattatt agttacggat tgaattatga acagaataga cgggaaagaa caattaaccc
     7201 aatagccaca taaacagtgc accaaacata atgccaaccg tcgccagagt gaaaacaata
     7261 ctgtagcgta gctttccgtc catcaatgaa tgcagtgcaa tccccaccac tacagcgacg
     7321 ggcatcagcg ccagaaagaa aggccaggtg tagataaaaa agagcagcgt gttagagcca
     7381 taaatcaaca tcggaattgc caacgcgcac agccacgata aaaaaccaac cacagcgcct
     7441 ggcagcgacc atgtggtgtc ttcaacctca gtaaggctgt cgttatttgt taatgtaatg
     7501 ttgtggctgt tacgcatatc tgatcctgtt actttgacga accgggcatg aaacccggtg
     7561 gtgtctcagg atctgataat atcgttctgt ctcaacagat ctaataattg ctgtaccaaa
     7621 tttgttacta attgttcacc attgagatga atttctgccg attcaggcgc ttcgtaaacg
     7681 gaatctattc ccgtaaagtt gcgcagttca ccggcacgcg ctttcttata taaacctttt
     7741 ggatcgcggg cttcgcaaat cgccagcggc gtatcgacaa acacttcgat aaagcgccct
     7801 tctcctacgc gttcgcgaac catctggcgt tcggcgcggt gtggcgagat aaatgcggtc
     7861 agcaccacca gtccggcttc aaccatcaaa ttcgccactt caccgacgcg acggatattc
     7921 tctttacggt cggcatcgct aaaaccgaga tcgctgcata atccgtggcg aacgttgtcg
     7981 ccatccagca gatacgtact gacgccaagt ttatgtaacg cctcctccag cgcccccgcg
     8041 acggttgatt taccggaacc agagaggccg gtaaaccaca gcacaacacc acgatgaccg
     8101 tggtgctgtt cgcgttgttg cagagttact gggtggctat gccagacgac gttttcgtca
     8161 tgcagagcca ttatttatct cccagtaaat cgcgcgcacc ccaatgcggg aaatggcggc
     8221 gaaccagggc attcaattcc agttcgaatg cactgaattc agatggcgca gcagttgcct
     8281 ggctaactgg ttcgtgcacc ataccggcac ctacggtcac attactcagg cgatcgataa
     8341 aaatcagccc accggtaacc gggttttgct gataacgatc taacaccagt ggctcgtcaa
     8401 aggtgagatc taccaggccg atgccgttca gcggcaggtt ttcaacttcg cgttgggtaa
     8461 ggttattgat atcaacctga taacgaatgc catcaacacg agcacgcgtc ttcttaccgg
     8521 caattttgat gtcgtaactc tggcccgggg aaagcggctg ttccgccatc cataccacat
     8581 ccaccgacgc gctctgcaca gccggtaaca cttcgtctgc cgccagcagc agatcgccac
     8641 ggctgatgtc gatctcatcc gtcagcacca gggtgatcgc ttctccggca aaggcttctt
     8701 cgcgatcgcc atcaaaggtc acgatccgcg cgacgtttga ttccacacca gagggcagca
     8761 ctttgacacg ttgcccgact tccacacgac cggatgccag cgttccggcg tagccacgaa
     8821 aatcgagatt cggacggtta acgtactgca ccgggaagcg cattggctga gcatccacca
     8881 ctcgctggat ctccacggtt tccagcactt cgagcagtgt cggaccgctg taccacggca
     8941 tactttcact ttgcgaagcc acgttgtcgc cttccagtgc ggagagcggc acaaagcgga
     9001 tatccagatt acccggcagc tgcccggcaa aggtcagata atcttcacga atacgggtga
     9061 acgtctcttc actgtaatcc accagatcca ttttgttgat cgccacgacc agatgtttga
     9121 tccccaacag tgtggagata aaactgtgac gacgggtttg atcgagcacg cctttacggg
     9181 catcgatcag taagatcgcc agttcacatg tcgatgcgcc agtcgccata ttgcgggtgt
     9241 actgctcgtg ccctggggtg tcggcgataa taaatttacg cttctcggta gagaaatagc
     9301 ggtaagccac gtcaatggtg atgccctgtt cgcgctcagc ttgcaggccg tccaccagca
     9361 gtgccagatc cagcttttcg ccctgggtgc cgtgacgctt actgtcgtta tgcagcgatg
     9421 agagctgatc ttcgtagatt tggcgggtat cgtgtagcag gcgaccaatc agggtacttt
     9481 tgccgtcatc gacgctacca caggtcagaa aacgcagcag gcttttatgt tgttgcgcaa
     9541 tcatccaggc ttcgacgccg ccttcattgg cgatttgttg tgcaagtgcg gtgttcatct
     9601 taaaaatacc cctgacgttt tttcagctcc atagaccccg cctggtcgcg gtcaattacg
     9661 cggccctgac gttcactggt ggtggaaacc agcatctctt cgatgatctc cggcagtgtt
     9721 tgtgcgtttg actccaccgc gccggtcagc ggccagcagc ccagcgtacg gaaacgcacc
     9781 atccgttttt taatcacttc gcccggttgc aggtcgatac ggttgtcatc aatcatcatc
     9841 aacataccgt cgcgttccag aaccggacgt tccgcagcga gatacagtgg aacaatgtca
     9901 atattttcca gccagatgta ttgccagata tcctgctcgg tccagttaga gagcgggaag
     9961 acgcggatgc tttcgccttt gttaatttgc ccgttgtagt tgtgccacag ctccgggcgc
    10021 tgatttttcg gatcccagcg atggaagcgg tcacggaaag agtagattcg ctctttagcg
    10081 cgggatttct cttcgtcgcg gcgcgcacca ccgaaggcgg catcaaaacc gtatttgttc
    10141 agcgcctgtt tcaggccttc agttttcata atatcggtat gtttcgcgct gccatgaacg
    10201 aatggattaa tccccatcgc tacgccttcc gggtttttat gcaccagcag ctcgcagccg
    10261 taggctttag ccgtacgatc gcggaactca tacatctcgc ggaatttcca gccggtatcg
    10321 acatgcagca acgggaaagg cagcgtacct ggataaaacg ccttgcgcgc cagatgcagc
    10381 atgacgctgg aatctttacc gatagagtag agcatcaccg gatttgagaa ttctgccgcc
    10441 acctcgcgaa taatgtggat gctttccgcc tccagttgcc gcaggtgggt aagtcgtttt
    10501 tgatccataa ccgttccttt gcaataccgc tattttcttg ccatcagatg tttcgactat
    10561 agggagcgta agagaacgaa tgaaattacc aattagaatg agtagttcct taacggaata
    10621 acgatttggc aaagctaata tcaaaaagtg cttaaggcac cggatttcgg gcgtttagga
    10681 agatttgaaa ttgttttagc gcagcggcag tttcatacta tggcggtaaa aaaatttgca
    10741 tggtatttaa ggactcacta tgttttccgc attgcgccac cgtaccgctg ccctggcgct
    10801 cggcgtatgc tttattctcc ccgtacacgc ctcgtcacct aaacctggcg attttgctaa
    10861 tacccaggca cgacatattg ctactttctt tccgggacgc atgaccggaa ctcctgcaga
    10921 aatgttatct gccgattata ttcgccaaca gtttcagcaa atgggttatc gcagtgatat
    10981 tcggacattt aatagtcggt atatttatac cgcccgcgat aatcgcaaga gctggcataa
    11041 cgtgacggga agtacggtga ttgccgctca tgaaggcaaa acgccgcagc agatcatcat
    11101 tatggcgcat ctggatactt acgccccgct gagcgatgct gacgccgatg ccaatctcgg
    11161 cgggctgacg ttacaaggaa tggatgataa cgccgcaggt ttaggtgtca tgctggaact
    11221 ggccgagcgc ctgaaaaata cgcctaccga gtatggtatt cgatttgtgg cgaccagcgg
    11281 cgaagaggaa ggaaaattag gcgctgagaa tttactcaag cggatgagtg acaccgaaaa
    11341 gaaaaatacg ctgctggtga ttaatctcga taacttaatt gttggcgata aattgtattt
    11401 caacagcggt gtaaaaaccc ctgaagcagt aaggaaatta acgcgcgacc gggcgctggc
    11461 aattgcccgc agccatggaa ttgccgccac cacaaatcca ggtttgaata aaaactatcc
    11521 gaaaggcacc gggtgttgta atgacgcaga aatattcgac aaagccggta ttgctgtact
    11581 ttcggtggaa gcgactaact ggaatcttgg caataaggat ggttatcagc aacgagcaaa
    11641 aacagccgca ttccctgcgg gaaatagctg gcatgacgta agactggata atcaacagca
    11701 tattgataag gcacttcctg gaagaataga acgtcgctgc cgtgatgtta tgcggataat
    11761 gctaccgttg gtgaaggagc tggcgaaggc atcttaatgg gtttgaaatg attactccct
    11821 tgcctgcagg aaatcaatgc caccagaaac agagtataaa aaaggggtgc aggaggccaa
    11881 accatcacaa ttcagtacgc ggggttttac cccgcgtggt ggctacttct tcgattcgta
    11941 agcgagaact gccttaaact ccataccacg ttctctgata ctcaactcac acaacgagtc
    12001 gcctaccatc aacgtgaatc ccagtactgt acaacacagt acgatgattg ccacaagggc
    12061 atattttgtc agcatgtctt gcctccctgc aaagaagaga ctaacatccg aattgctacg
    12121 ttcgaacgcg agcctcaggt tgatagaaat atcgcctggg gcttttgtcc atctggaacc
    12181 ttgcgaatgc ttaacgccag acagcctcaa gcacccaatg ccattctata cctgttaatt
    12241 ttccctgcga tgttcacctt taaatgccgg aatgctgctc gcaaaaaata aaaacgcctc
    12301 catcatttct gacagaggcg tttaatttat ccggcaatat ttacccttcg tgtaatccac
    12361 attccctttt taagccaaag aaacgcgttt cttcttccgc catgccgggt tcccatttac
    12421 gggttgtatg ggtatcgccc accgataaat atccttcatc ccataatggg tgatatttca
    12481 ggccatgttt ttgcaggtac tgataaatag ttcggttatc ccagtcgata atcggcagca
    12541 ctttaaatac gccacgctga attgccagca ccggtaaatt ggcgcggctg ccggattgtt
    12601 cacggcgcag gccagcaaac caggtttgcg cattcagttc tttcagcgcc cggttcatcg
    12661 gttcgacttt gttgatgtca ttgtactttt caatgccttc aacgccctgc tcccacagtt
    12721 tgccgtagcg tgcttcctgc caggcggcgc tttcggtagc acgatacact ttcaggttga
    12781 gcttgagttt gtccgttaac tcgtcaataa agcggtaggt ttccgggaac agatagccgg
    12841 tatcggtgag gatcactgga atatccgggc gaatctgatt caccagatgc aggctcaccg
    12901 ccgcctgaat gccaaagctg gaagaaagta catattcacc gggcagatta tccagcgccc
    12961 aggctacgcg gccttcagcg tccagttttt ccagttgggc gttagtttcc gccagcgcca
    13021 gaatccggtc tacctttggc agttcgttca gggcgtttag atcgagtttg gacataagtt
    13081 cctcactgtt tgccttgcct gatgcaacgc tcacacgtct tatcaggcct acaagaccgg
    13141 gctgatggtt aatcccacaa atcgcgcgcc ggatcgagca ccgggcgaat gatgcccgca
    13201 cgcaccgtaa agtcgccgaa gccttcaccc gcttcgcgct ctttcgccca gcgccctatc
    13261 agttcatcaa gcgacgccag gatttccggc tcggtgatgt tttctttata catccgtggg
    13321 atacgtgtcc caatgcggtt gccgccaaga tgcaggttgt agcgacccgg cgctttcccc
    13381 accagaccca cttccgccag catcgcgcga ccacaaccgt tcgggcagcc tgttacacgc
    13441 atcacgatat gctcatcgct gacaccatgt ttcgccatta aattatcgat gttgtcgata
    13501 aaagacggca ggaaacgctc cgcttccgcc atcgccagcg ggcaagtcgg gaatgacacg
    13561 caggccattg agttttcacg ctgcggcgtg acggcattca ttaacccgct ttctttggcg
    13621 atcttctcga tcttcgcttt ctcgctttcc ggcacgccgg cgatgatcag attctggttc
    13681 gccgtaatgc ggaaatcacc tttgtggatc ttcgcgatct ccagcaagcc ggttttcagc
    13741 gggcgccccg gataatcaag aattcgacca ttttcgataa acagcgtcag gtgccagtta
    13801 tcatcaatgc ccttaaccca gccaatacga tcgccgcgtc cggtaaactc atacgggcga
    13861 atcggctcga atttgatccc cgcgcgacgt tcaacttccg ctttaaacgt ctcaacccct
    13921 acgcgctcca gcgtgtattt ggttttggca tttttacgat cggttcggtt accccagtca
    13981 cgctgagttg tcacgacggc ttccgccacc gccagcgtat gctccagcgg cagatagcca
    14041 aactcactcg ccgtgcgggc gtaggttttc ttgttgccgt gttcgatgga aagcccaccg
    14101 cccaccagca ggttaaagcc caccagcttg ccgttttcgg cgatcgccac gaagttcatg
    14161 tcgttggcgt gcagatcgat atcgttctgc ggcgggatca ctaccgtggt tttgaactta
    14221 cgcggcaggt aggtctgacc aaggatcggc tcttcatcag tagtggcgac tttttcctga
    14281 tcgagccaga tctccgcata cgcgcgggta cgaggcagca gatgttcaga aattttcttc
    14341 gcccattcat atgcttccgc atgcagctgc gactcgtaag ggttcgaggt gcagagtacg
    14401 ttacggttca tgtcgttggc ggtcgccagc gcatcaagac cgaccgagtg cagcatctgg
    14461 tgcaccggtt tgacgttctt tttcagaatg ccgtggaact gaaacgtctg gcggttggtc
    14521 aggcgaatgc tgccatagat ggtgttttca ccggcaaatt tgtcgatcgc ctgccactgt
    14581 ttagtggtaa tcaccccacc cggcagacga cagcgaagca gcatcgcgtg gcgcggctcc
    14641 agcttctgtt cagcacgttc ggcgcgaatg tcgcggtcat cctgctgata catgccgtgg
    14701 aagcggatca gcaggaagtt atcgccctta aagccgccgg tcagaccgtc gtttaaatct
    14761 tccgcaatgg tgccgcgcag gtagttgctt tcaagcttca tgcgctcggc gtctgtcagt
    14821 tttccttcga ccactaaagg ccctggatgt ttttcgctca ttagtagaca tctcgctgat
    14881 aacggcgctc tacgcgcagc tcacttaaaa attcatccgc cgcttcggtg tccatgccac
    14941 caaattcggc aatcacttcc agaagtgcct gctcaacgtc tttcgccatg cgattagcgt
    15001 cgccgcagac ataaatgtgg gcaccgtcat tgatccagcg ccacagctct gcgccctgtt
    15061 cgcgcagttt gtcttgtacg taaatttttt ctttttgatc gcgcgaccag gcgagatcga
    15121 tgcgcgtcag cacgccttct ttgacgtaac gctgccattc cacctggtag aggaaatctt
    15181 ccgtaaagtg tgggttgcca aagaatagcc agtttttacc cggcgcttcg tcggcggcac
    15241 gctgctgcat aaaggcacgg aacggcgcga tgccggtgcc agggccaatc atgatcaccg
    15301 gggtttccgg attggttggc aggcggaagt tgtcgttatg ttcgataaat acgcggactt
    15361 caccctcttc ttccacacga tccgcgagga agctggaggc accaccggca cgggcgcggc
    15421 cttccacgtc gtaacgcacc acaccaacgg taacgtgcac ttcgttctcg acttccgcct
    15481 gcgaggaggc gatggaatac aggcgcgggg tcagtgggcg cagcagatta atcagcgctt
    15541 cggcatccag ttgcgccgga gagaaacgca ccatgtcgac aatcggcgtc gtcgcggcgt
    15601 aatgctgtaa cttcgcttta tcgcccacca gcggcagcag cgtttcgctg cgggtaagcg
    15661 tggcatagtt ctcaacaatg ttggcggtgt tgacggtcag ttcgaagtgc cactgtagcg
    15721 cttcgttcag aggcaacgtt ttgccctcga cggtgacagg ttcatcgcct ttcagccaca
    15781 gcagttcgac aagttctttc accagtgccg gatcgttttg ataccagacg cccagcgcgt
    15841 cacccggctg gtaacgcagg tcagagtcac ctaagtcaat ttcaatgtga cgaacgtctt
    15901 tttcagagtt acgcccggta attttctggt taacagagag gctcgccacc agcggcgcgt
    15961 ctttgctata cgggctggtg tggatttcat ttaccgtgcc agtagcgacg gattgcgaag
    16021 gtgccgcgac aggcgcacgc gatttaagcg catcaaccac gcgggcgcgc cactcgctgg
    16081 cagcagcctg gtattcaacg tcggcatcga cacggtcgag caggcgttca ccgcccagtt
    16141 ccgccagctt actgtcaaaa tctttcccgg actggcagaa aaattcataa gagctatcgc
    16201 cgaggctaaa cacggcaaac gcggtgtttt ccagctttgg cgcttttttg gagaacagga
    16261 acttatgcag cgcgacggct tcttccggcg gttccccttc cccttgcgtt gacgtcacca
    16321 cgataagcag tttttcgctg gcgatttgtt tgaatttata gtcgcccgcg ttcaccagct
    16381 taacgttcag ttttgccgct aataaatcat cgcgtaatgc ttcagcaacc cgacgcgcat
    16441 tgccagtttg cgaggcggag ataatagtta tacccggcat ttctgcggcg ggcgctggcg
    16501 tcgctgcaag cgcagcaggc tgctggttga gcacgcccca gaaatagcca gaaacccagg
    16561 caagctgggt aggagttaaa tcggtcgtgg ccgcctgaag gcgtgccagt tgctccgggt
    16621 tcaacggaag caacgcggaa ggtgggacct gtgtcgtcat gcgtcgttat gttccagtaa
    16681 gcaaagctgt ttctgcgccc tgtcagcgcc cataaaacag aagagaaggt aaggttaacg
    16741 gggtgaacgg tgtggattaa agacgggata gcgataacta ataaccaaat cgactaacct
    16801 gttttagcaa tagtctttaa caacaaaata gattaaccaa cccaatgaaa aacaaatgaa
    16861 tattgcccat caataagata aatcagcgat tttgcgcaac aaagtcgttt tagataatgc
    16921 gaaaaaacag cctttccggt actctacggc ggttttatcg tcatgtagag aaattatgat
    16981 gtccaccacg ttatttaaag atttcacctt cgaagccgct caccgcttgc cgcacgtccc
    17041 acaagggcat aaatgtggtc gcctgcacgg gcattccttt atggtgcgac tggaaattac
    17101 cggggaagtc gatccgcata cgggctggat tatcgatttc gctgaactga aagcggcgtt
    17161 taaaccaact tatgagcgcc tcgatcacca ttatctcaat gatattccag gtctggaaaa
    17221 cccaaccagc gaagttttag caaaatggat ttgggatcag gttaaacccg ttgtgccgct
    17281 gttaagtgcg gtgatggtaa aagaaacctg caccgcaggt tgtatctatc gcggcgaatg
    17341 ataagagtgt gtcggcggtc aatttccctt aagtaacgct atgttagggt gttgtgttct
    17401 ggatatctgg ggcatgacat ggaagacgac tgcgacatta ttattatcgg tgccggaatt
    17461 gctggcaccg cttgcgcgtt acgctgcgcg cgagcgggtt tatccgtttt gttactggaa
    17521 cgagcggaga ttcccggcag caaaaatctt tccggcgggc gtttatatac ccatgcgctc
    17581 gcggaactcc tcccgcaatt tcacctgacc gcgccccttg aacgacgcat cactcacgaa
    17641 agtctttctc tgttaacgcc agatggcgca acgacgtttt ccagcttaca gcccggcggt
    17701 gaatcctgga gtgtattacg tgcacgattc gatccgtggc tggttgccga agccgaaaaa
    17761 gaaggtgtcg aatgcatccc cggtgcgacg gtagatgcac tgtatgaaga aaacggcagg
    17821 gtgtgtggtg tcatttgtgg tgacgatatt cttcacgccc gttatgtggt gctggcagaa
    17881 ggtgccaaca gcgtcctggc ggaacgccac gggttagtga ctcgtcctgc tggcgaagcg
    17941 atggcattgg gaatcaaaga agtgctgacg ctggaaccat ccgctattga agaacgtttt
    18001 catctggaga ataacgaagg cgcagcgttg ttgttcagcg gcgggatctg tgatgactta
    18061 cccggcggcg catttcttta tactaatcaa caaacgctct cgttagggat tgtttgcccg
    18121 ctctcctccc ttacgcaaag tagtgttccg gcaagcgagc tgctgactcg ctttaaaacg
    18181 catccggcag tgcgcccgct tatcaaaaac acggaattac tggagtatgg tgcgcatctg
    18241 gtgccagaag gtggcctgcg cagtatgccg gtgcaatacg ccggtaacgg ctggctgctg
    18301 gtgggcgatg cgttgcgcag ttgcgtcaat accggaattt ccgtgcgcgg catggatatg
    18361 gcgctaactg gcgcgcaggc ggcggcacaa acgctgataa gcgcctgcca acaccgcgag
    18421 ccgcaaaatc tgtttccgct ttatcatcac aacgtagagc gcagcctgct gtgggatgtt
    18481 ctacagcgtt atcagcatgt tccggcgctt ttgcaacgtc ctggctggta tcgggtgtgg
    18541 cctgggttaa tgcaggatat ttcccgtgat ttatgggatc aggggaatac acctgttcca
    18601 ccgctgcgcc agttgctctg gcgtcattta cgtcgtcacg gcctgtggca tctgacgggc
    18661 gatgttatca ggagtctgcg atgtctgtag cccgtaatct ctggcgcgct gctgatgcgc
    18721 cgcacattgt tccgactgac tccgttgagc gccagacggc acaacggttg attaacgcct
    18781 gtccagcagg tcttttttcg ctcacacctg aaggtggctt acgtgttgat tatcacggct
    18841 gcctggagtg tggcacctgc cgtttgctgt gcgacgaatc aacactacaa cagtggcgct
    18901 atccgccttc cggattcggc atcacctacc gctttggata aataataagg ataatttatg
    18961 cccctcttac acctgctccg ccagaatccg gtgattgctg ccgttaaaga taatgccagc
    19021 ctgcaactgg caatcgactc tgaatgccag tttatttccg tgttgtacgg caatatctgc
    19081 accattagta atatcgttaa aaagattaag aacgccggga aatatgcttt tattcatgtt
    19141 gatttgctgg aaggcgcgtc aaataaagaa gtggtgattc agtttttgaa actggtaacc
    19201 gaggcggacg gaattatcag taccaaagcc tcaatgctga aagcagcaag agcggaaggt
    19261 tttttctgta ttcatcgact gtttattgtt gattcgattt cgtttcacaa cattgataag
    19321 caagttgcgc aatcgaatcc ggattgtatt gagatcctgc caggctgcat gcctaaagtg
    19381 ctgggctggg tgacggagaa aatccgccaa ccgctgattg ctggcgggct ggtgtgcgat
    19441 gaagaagatg cgcgtaatgc gattaacgcg ggtgttgtgg cgctttccac cacgaatact
    19501 ggggtctgga cgttagcgaa aaaattgctt tgacgggata atcgtaacaa gttgaatttg
    19561 gtgtgatttg taggctgcac gccacatccg acattcagcg cctgatgcga cgcttgacgc
    19621 gtcttatcag gcctacaagt ccccagccca gaaccatcgg caggatcatc cggcagcacg
    19681 ttattggcaa tctgcgtgaa tgtttgtcac cagtgcctcc agcaccactt tccagtcatc
    19741 aactacgcca acatctgcct gtgaaaatac cgctgcactg gcgtcatgat taatcgccac
    19801 cacaaacttg ctattgcgta cgcccgccat taacgctgct gcgccggaag cgccaacaac
    19861 aatacacacc tcaggtgcca gcagatgtcc ggaaatacca atcacttttt cggcatcaac
    19921 gccaccgttc atcactcgtg cccgactgta gcccacttcg gctcccatct tttctgccag
    19981 catagcgatt tcctggttat ctgcctctcc gccctgcccc accaccagca cccgtctggc
    20041 ttcagctaat gggtcgcgga taacattttt cagattttct atgctaacca gccagtcggg
    20101 aagcacacct gggacaatgt tcagttgctg catcccagag ggcaacgtgg cgtttttatc
    20161 tgctcccgcc tgacgtgcca gcgaaaggca tagcgggcgt ttgtctgttt gcagcgtcgc
    20221 cgtcaacgca tttccccagt gggatttgcg cacgcttacg gttggaatat ctaacgaagt
    20281 cacctggcag atgcttgcac catgtaaacg ccaggccagc cgggtcgaca gttcatcgcc
    20341 aaacgcgccc ggaggaaaaa ggacaacgtc cgcaggcgtt cgttgccact gttccaccag
    20401 tgcatccagc acctgttccg caaccatcgg ctgtggttct atttgccaat gtgccagggt
    20461 acaaccgctg aaatcctgcg ccgccagcca gctcgctatt gcggcatggt cctgatttat
    20521 tgtcacaatt acgatattca tggctgcatc ctttggcgta aatagtcctg ccatagcttt
    20581 tgcgcttttt ctgctaccgt ttgcccacca atcagcgtcg ctccgcgtcg ttgctctgct
    20641 cttgccagtt gcagacactg catcgctggc aattcgacag caacagtttt acggataatt
    20701 gctgctttcc ccgcagccat tcgctggcgc attcccggaa ctggcaaggc cacttcaccg
    20761 cactgacgca cggcaatcac cgcaggcaag cgaacccggc aacagcgcac cccatgttca
    20821 gtacgctgtt cgagggtgat aaacggtgcg tcgagagtaa agcgctccac ctgggtgaag
    20881 cagggccaga ccagcatttc cgccagcaaa aatggcgttt gcccattctg cccttcgctg
    20941 ctttggcagc cggtaattat cagatccagc ggattctgac gctgccattc ggcaatatgg
    21001 cgagcgacaa attccggcgc aaagcgcaga tctgccgccg tctccagcaa tacagcttcc
    21061 tcaaacccaa gagccatgag atagcgcagc cagtgcaacg cccgttcatc ccccatgctc
    21121 aacgcggtta aagacatcgg cgtaccgttt tttctctgcg ccaacagcag cgcagctgcg
    21181 gcctgttcat cagcaccgag taaacttcgc agaagcgaaa catccggtcc gctattaccc
    21241 tgagccgccg cctgccactc tttttccgcc agcattccgg catccggttc ggctttaaac
    21301 gctaacagaa tgttcattcg cacctcctac atttcccgcc gccaccagcg ggagtgcttt
    21361 ggtttctggt gcccatagcc aggtcacaac aaaaccaacc aacaacactg ccgccaggag
    21421 taataaggtg gcttgcattc cccactgcgc cagcacccac ggcagcaggc cagtacttac
    21481 cgccgccccc aaccgactca tggcggtggc aaaaccgacg cccagcgagc gaatgtcagt
    21541 aggaaaactt tccgcaggca aaatgcccac cagattactg actgccgaaa tggtggtgct
    21601 gaagagaaca aaaagcagca gcgttaatga actgccggaa ggcaaacagg ccatcactac
    21661 cagcgttgcc gccagcagca aaaaacttcc cagcaaaaac ttgcgatgtg ccagcaggtg
    21721 cgtcagaact aatcccagca gcgcgcccac aattaacaac gcattaagca tcagactggc
    21781 agtcagcgca tcttccagtc caatagtctg ggcgatagtt ggcagccagg tataaatcac
    21841 aaaccatggg attacgaggc agacaaagaa gacgctgtta aacgctgtgc gccgccagta
    21901 gcgcgaagag aacaaggttt tgatgtgttt atgggtcgcc gttgccactt catcgcccag
    21961 taaaacatgc gggccaaaat agcgatgaac gatagcgtga gcttctgcaa aacgcccctg
    22021 gcgcagtagc cagcgtggcg attccggcgt gccccaacgt agcagcgtaa tcagcaaggc
    22081 tggtaacgcc gctgatgcca gcagccagcg ccaggcctcc gggctttcgg aaataaagtg
    22141 atgtccggca atacttgcca gcacatagcc tacggtccac accacactga atgcgcccag
    22201 caaaataccg cgatggcggc gcggggaaaa ttcagccagc aaggtgtgac cgactgagta
    22261 atcgcctccc agaccaatgc cgatcaaaat gcgtaatcca ataagatgct ctggcgtggt
    22321 ggcaaaaaat tgcaagaacg aagcgagcgt aatcagcata aaactgaagg tgaagatttt
    22381 ttgccgcccg atatggtcgg agatccaccc caaaaccagg ctgccaagaa acaaaccaag
    22441 taacgccgag ccgccgatca ttcccgccat aaacggcgtc agttgcatgg cgggcgtaag
    22501 ctgaataatg gcataaccaa tgacgccgag aacataaccg tcggtcaggt gtgcgccgaa
    22561 agtgagcgca gcaattcgac agtgaaaacg gttaagcggt aaatcatcca ttcgcaccgg
    22621 tgaagtgttc atctttaccc cttcattgtc cggtaataag aacggcttct cgcctgagaa
    22681 gccgaaaagg caacgcaccg tcatcgcgga acgctgcctg acacattatt tttcaatcgg
    22741 atagatagta cccgtgttca taatgccatt aggatcgaac tgctttttca gcccttccag
    22801 caacgcccac gcgctgccgt gttccagttt gctccagtga acgcgatgtt taccgatacc
    22861 gtggtgatgc accatcgaac cgccgaggcg aatggtttct tcgcagatga ttttgttgag
    22921 cggattgtgg tacttgtcga tttcctcttc cggcttacag tcaacgacgt tgtagtcgta
    22981 gacgaagtac atgttggtgc cgttctgata gctatgagag gagtgaccgc ccagcatggt
    23041 gatgtcgtcg gcgtgcggga actcggtacg aatacggtta ataacgcttt cgtagatttc
    23101 gtgaatgcag ctccagcagc cggacacttc agtggtaaag cccatgttgc cggttttgag
    23161 gatctgcaca cgttcggcag ccactttatc cggtccccag ttcaggttgt taaaccaggt
    23221 ttcgatcagc ttgctgtcca cgcgctggca ctgcgggtag cgcgccacga tttcagcaat
    23281 cccttcaccc gtcgccttcg caatgcgagg gttaccttca gccataaaga tcagcacgca
    23341 tttgccgtcg gcaaaatggg tgaagtgttg ggtgccatct tcagcatcat acaaacgagc
    23401 aatcgacgga cgataccctt ccaccatgat ttcacgcagg atgttgaagc cggttttcat
    23461 atcttccagg atatagccgt agaagagatt gttttccggc gtaaatttga agattttcac
    23521 tgtgacttca gtgatatagc acagtgcacc ttcgttgccg atgatgatat gacgaatgtc
    23581 cgggccagcc gcgcgacgtg gcactttttt aatgcgtgtg acggtgccat ctgccagcac
    23641 cgcttccaga ccaacgacca tatcttcgat tgcgccgtag agtgtggaga actgcccgat
    23701 actgcgggtc gccaccaagc cgcccatctg cgccagcggc tttgactgcg gagaatgccc
    23761 cgtggtgtaa cctttttcgc gcaatgcgtt ttccagcacc tccagcggaa cgccacattg
    23821 cgccgtggcc tgcatattct caatatcaat attaatgatt tgattcatgg cggagccgtc
    23881 gagcaccacc gagttttcca caacggtttc cagtccacct tcggtggcgg aagcaccggt
    23941 acgtggcaca ccgttaattt tgtgcgcatt cataaaattc agcacacggg atacttgttc
    24001 tgtggaaccg agttttacga ccgctgccgg aatcggcaaa gtataaattc catgaatatc
    24061 cggaaattta cgaaaacggt caatactgtt tttctttaat accgtttcat cggtaataac
    24121 gcgatctgca ccaacaattt cctttagctg gtcgacaatc gctacgcgag ataaagacat
    24181 aataattcct tcctgataat aaaaatggaa tagttattaa taagagaaaa cagaaaccac
    24241 cgttatgaat tagcgcacta aataaccgcc atcaaccact aataaatgtc cgttgacata
    24301 attcgatgcc ggacttgcga ggaatacggc tgcccccatt aaatcctgag tatcacccca
    24361 acggtttgcc ggaatatgat caagaacacg ctggttagtt tctggattac tacgggtcgc
    24421 cagcgtaata tcggttgcat aataaccagg agcaatacca ttcacctgaa tattatattg
    24481 gcctaattca tcgcaatagg ctttggtgaa tccggcaaga gcatgtttag tggcagaata
    24541 agcaggtgac cattgaccac ctaagtaaga gaacaatgaa cagatattaa tgattttacc
    24601 gcttttctgc gggatcataa tttttgccgc ttcgtagctt aactcgaatg cggcggtcag
    24661 gttcacgtca atcatcgggt cccagtcggc acgaccaaag tccagcacct tattcagctt
    24721 acaaataccg gcattattga ccagaatatc gacggtaccg aagcgctcac agcaggcagc
    24781 gataatcttc tgcggcgcgc cttctgcggt gatatccacc tgcataaaat caacttcaac
    24841 gccctgtttt tcaatcattt cctttgtttc gccgttatct ttgacgaaac taggaataaa
    24901 gacatttgcg ccagctttgg ccaacgccat ggcaaatgcc tggcctaaac cgctattccc
    24961 gccggtaaca attgctgttt tacctttcag ggagaaaaaa tccattgaga acgcattgag
    25021 agattcgatt gacatagtta gctccaattt ccgtaaggca aaaaaaaaga aaggtaactt
    25081 cccccgcgag agggaagtta cctttctcat tatctctggt aactggagat aatttttagc
    25141 ataaggacat aactgctaat gtgatcgtaa tcacagtgtg atgttcgtat tgtcaaataa
    25201 tttaaaaaag cagaagcgtg attactctca cacaacacct ctgctttcca tgttatttct
    25261 tattgcaatt actagagacc tgagaaaagt aatccacctg aaagggcggc ttacttttct
    25321 cttttttttg tctattacaa atgggaatac gatatgcaac acaactcata tcgccgttgg
    25381 ataaccctcg cgataattag ttttagcggc ggcgttagtt tcgacctggc ttatttacgt
    25441 tatatttatc aaattcccat ggcaaaattt atgggattca gcaataccga gataggttta
    25501 ataatgagta cctttggtat tgcggccatt attttctatg cccctagcgg cgttattgcc
    25561 gataaatttt cacatcgcaa aatgattact tccgcgatga tcattaccgg attactgggt
    25621 ctgttaatgg caacatatcc accgctgtgg gtaatgctct gtattcaggt cgcctttgcg
    25681 ataacgacga ttttaatgct gtggtcggtg tcgattaaag ccgcatcgtt gctgggcgat
    25741 catagcgagc aagggaaaat tatgggctgg atggaagggc tgcgcggcgt cggtgtaatg
    25801 tcgctggcgg tgtttaccat gtgggtcttt tctcgctttg caccggatga cagcgccagc
    25861 ctgaaaacgg tcattattat ctatagtgtg gtttacattc tgctgggcat tttgtgttgg
    25921 ttttttgtta gcgataacaa caacctacgc agtgccaata acgaagaaaa acagtcattc
    25981 cagcttagcg acatcctggc cgttttgcgc atcagcacta cctggtattg cagcatggtg
    26041 atttttggtg tcttcaccat ctacgccatt ctgagttact ccaccaacta tctgaccgaa
    26101 atgtacggca tgtcgctggt ggcggcgagc tacatgggga ttgtgatcaa caaaatcttc
    26161 cgcgcgctgt gcggcccact tggcggcatt atcaccacct acagtaaagt gaaatcccct
    26221 acccgcgtgg tccaaatcct ttccatcatc ggtttgctgg cgttgacagc cctgctcgtc
    26281 acgaactcta acccgcaatc ggtcgcgatg gggattggct tgattttatt actgggattc
    26341 acctgttacg cctcacgcgg gctgtactgg gcctgccctg gcgaagcgag aacaccgtct
    26401 tacattatgg gcaccacggt aggtatttgt tcggtgattg gattcctgcc ggatgtcttc
    26461 gtttacccga ttatcggcta ctggcaagac accctgcccg ctgcagaagc ctatcgcaac
    26521 atgtggctaa tgggcatggc ggcgcttggc atggtgatca tctttacctt tttgctgttc
    26581 caaaaaattc gtactgctga tagcgccccc gcaatggcta gcagcaagta agccaggaaa
    26641 attacaggag aaaaatcatg tcgaaaaaat acatcatagg gattgatggc ggaagtcaga
    26701 gcacaaaagt ggtgatgtac gatctggaag gtaatgtggt ttgcgaaggc aaaggcttat
    26761 tgcagccgat gcacacgcca gatgccgata ccgcagaaca tcctgacgat gatttatggg
    26821 catcactatg ttttgccagt cacgatttga tgagccagtt tgccgggaat aaagaagata
    26881 ttgtcggcat tggcctgggc tcaatccgtt gctgccgtgc gctattgaaa gccgatggta
    26941 cgccagccgc gccgttgatt agctggcagg atgcacgcgt tacacgccct tacgaacaca
    27001 ctaatcctga cgtggcctat gtcacctctt tttcgggtta tctgacacat cgcttaaccg
    27061 gcgagtttaa agacaatatc gccaactatt ttggtcagtg gccggtggat tataagagct
    27121 gggcatggag cgaagatgct acggtaatgg aaaagtttaa tatcccccgc cagatgcttt
    27181 ttgacgtgca aatgccgggc accattcttg ggcatatcac accacaagct gcgctggcga
    27241 cacatttccc ggctgggctg ccggttgttt gtaccaccag tgataaaccg gtagaagctc
    27301 tgggagccgg gttactggat gatgaaacgg cggtaatttc tcttggcacc tatatcgcgt
    27361 tgatgatgaa cggcaaagca ctgccgaaag atccggtggc gtactggccg attatgtctt
    27421 ctattccgca aacattgctg tatgaaggtt acggtattcg caaaggcatg tggacggtga
    27481 gctggctgcg cgacatgtta ggcgagtcgt taattcagga tgccaaagca caggatcttt
    27541 caccggaaga tttgctcaac aaaaaagcgt cttgcgtgcc gcctggctgt aatggtctga
    27601 tgacggtgct ggactggctg accaatccgt gggaaccgta caaacgcggg attatgatcg
    27661 gttttgattc cagcatggat tacgcatgga tatatcgttc gatactggaa agcgtggcgc
    27721 tgacgctgaa gaataattac gacaatatgt gtcatgaaat gaatcacttt gcaaagcatg
    27781 tgatcattac tggcggcggt tcgaacagcg atctgtttat gcagattttt gccgacgtgt
    27841 tcaaccttcc ggcacgccga aacgccatta acggttgtgc aagtctgggg gctgcgatca
    27901 atacggcggt aggtctgggg ctatacccgg attacacaac ggctgtcgat aaaatggttc
    27961 gcgtgaaaga tatcttcata ccgattgaga gcaatgccaa acgctacgac gcgatgaata
    28021 aaggcatttt caaagaccta accaaacata ctgatgtgat cctgaaaaaa tcgtatgaag
    28081 tgatgcatgg ggaattgggg aatgcggatt cgatccagag ctggtcgaat gcgtaagtga
    28141 gataaacagt gccgggtaga tgattaatcc cggcatattc acgtttccac tacaaaatac
    28201 cgggtaacca ctgctgaata ttgttctctc ctgagcagcg tttatgcttt aattgctaaa
    28261 ccgatttaat tagtttagca attagtatat tggagccttt atgagcacaa gagtatgggt
    28321 actcggtgat gcggttgttg atttattacc cgaaagccag gggagactac tacagtgtcc
    28381 tggtggggcg cctgctaatg ttgcagtcgg tatcgcaagg ctggggggga aaagtgcctt
    28441 tattggcaaa gttggcgatg atcctttcgg tcgctttatg tatcaaacac tgagtacaga
    28501 aaatgttgat acacgttata tgtctcttga tcctcaacat cgcacctcaa ttgtggctgt
    28561 aggacttgat gagcaaggag aaagaaactt tacctttatg gtacgcccaa gtgccgatct
    28621 ttttttacaa cctgatgacc ttcctgcatt tgggccgggt gaatggctcc atctttgttc
    28681 cattgcgctc agtgcagaac cttcccgaag taccgcattt ctggctatgg agaaaatacg
    28741 tcaggctggc ggaaacatca gttttgatcc caatatccgc agcgatctct ggcagagtga
    28801 agcgctgtta aggaaatacc ttgatcgtgc gctttcgctg gcgaatatcg ctaaattgtc
    28861 cgaagaagag ttgttattca tcagtgacga aagccaggtt cagcaaggcg catattcatt
    28921 agtacaacgt tatccgttga ctttattgct tattacacaa ggaaaaaatg gcgtacttgt
    28981 gtattttcag gggcagttta tccactatcc cgccaaacct gtttctgtcg tcgatacgac
    29041 cggggcagga gatgcttttg tcgctggatt acttgccggt ctggctgatt ctggaatacc
    29101 aacaaatacc agacagcttg aacgaatcat tgcacaagct cagatttgtg gtgctctggc
    29161 gaccacggct aaaggcgcga tgaccgcctt accccgacaa cacgatctcc cttcacaata
    29221 gtgatcctgc tgttgtatct gtaacccgat ttgtcgtgcc tgtcacattt actaaatcgg
    29281 tttagcgaaa ataattgctc tggctaaaaa gtcagcttta ttatcaacac ctaaaccggt
    29341 ttagcatttt agcaattttt ataattctct gatgggacgc gacaaattat gtataaaaaa
    29401 acaactttgg cagtgttaat tgctttgctg accggtgcta caacggtaca tgcgcaaacg
    29461 gatattagca gtatcgaatc tcgactggcg gcgttggaac aacgtttaaa aaatgcggaa
    29521 tcccgcgccc aggcggcaga agcaagggcc aaaacagctg aattacaggt tcagaaactg
    29581 gctgaaacac aacaacaaaa tcagctaaca actcaagaag tagcacagag aacagttcag
    29641 ctcgaacaga aatccgcaga aaacagtggt tttgagtttc atggctatgc ccgttccggg
    29701 ttactgatga atgatgccgc ttccagtagc aaaagtgggc cgtatctgac tcccgcaggt
    29761 gaaactggtg gagctgttgg ccgtctggga aatgaagccg atacctatgt cgagttaaat
    29821 gtagaacata aacaaacact ggataacggt gcgaccacac gctttaaagc aatgttggct
    29881 gacggacaaa gagattacaa cgactggact ggcggctcca gtaacctgaa tatccgacag
    29941 gcttttgccg aactgggcgc attaccaagt tttaccggag cattcaaaga cagtactgtc
    30001 tgggctggta aacgctttga tcgcgacaat tttgatattc actggttaga ctccgatgtc
    30061 gtatttttag cgggaacggg cggcggtatc tatgacgtaa aatggaacga gacattccgc
    30121 agtaactttt ctctctacgg acgtaatttc ggcgatcttg atgatatcga caataacgtt
    30181 cagaactaca tcctcaccat gaatcattat gcaggcccct tccagttgat ggttagcgga
    30241 ttacgggcaa aagataatga tgatcgaaaa gatgccaatg gtgatctcat tcaaactgat
    30301 gctgcaaata ctggcgtaca tgcgttagtt ggtctgcaca atgacacttt ctatggcctg
    30361 cgtgaaggga cggcaaaaac agcactgcta tatggccatg gcctgggtgc ggaagtcaaa
    30421 gggattggct ccgatggcgc tctgctgtct gaggcgaata cctggcgctt cgcatcttac
    30481 ggcacaacac ctctgggaag cggttggtat gttgcgccag caattctcgc acaaagcagt
    30541 aaagatcgtt acgtcaaagg cgatagctac gaatgggtga ccttcaatac acgtctgatc
    30601 aaagaggtaa cacagaattt tgctctggcc tttgagggta gctatcaata tatggatctg
    30661 aagccaaagg ggtatcaaaa ccacaacgcc gtaaacggca gcttctataa actcaccttt
    30721 gctccaactc taaaagctaa cgatatcaat aatttcttta gccgtccgga gcttcgcctg
    30781 tttgccacct ggatggactg gagcagcaaa cttgatgatt ttgccagcaa tgatgctttc
    30841 ggcagcagtg gtttcaatac tggcggagag tggaattttg gtgtccaaat ggaaacctgg
    30901 ttttaatacc agacagcctc acggatgtga ggcttttact gaaataacaa tgaattagag
    30961 ggttatatgg atttcgataa aatcgcccaa tcgttgcttc ctttattagg tggtaaggag
    31021 aacatcgcca gtgcagcaca ttgcgccact cgcctacggc tggtactggt tgacgacaca
    31081 cttgccgatc aacatgccat tggccagatt gatggagtga aaggttgttt tcgtaattca
    31141 gggcaaatgc aaatcatctt tggcaccggc gtggttaata aagtctatgc tgcctttatt
    31201 caggttgcag gtattagcga atccagtaaa gcagataccg ctcgactcgc cgctcaaaaa
    31261 ctcaatcctt ttcagcgaat agcacggcta ctttctaata tttttgtccc catcattcca
    31321 gccattgttg cttctggttt attaatgggg cttctgggaa tggtgaaaac atatggctgg
    31381 gtgaatgccg ataatgcgat ttatatcctg ctggatatgt gcagttcagc cgcatttatt
    31441 attctcccca tccttattgg ctttactgct gctcgtgaat ttggtggaaa tccttatctt
    31501 ggcgcgacat taggagggat tctgactcac ccggcactca caaatgcctg gggcgttgct
    31561 gcgggatttc agacaatgaa cttcttcggt tttgaaatcg ccatgattgg ctatcaggga
    31621 acggttttcc ccgtgcttct ggcagtatgg tttatgagca tagtggaaaa acagttacgc
    31681 aggtttatcc ctgatgctct ggatctcatt ctgacgccat ttctgactgt cgtcatttct
    31741 ggctttatcg ctcttttgat tattggtccg gcaggacgag cgttaggtga cggtatctct
    31801 ttcgttctca gcacgctgat tgcacacgca ggctggttag cagggttgtt attcggcggg
    31861 ctatattcag cgattgttat tacgggtatt catcatagct tccacgcaat tgaagcagga
    31921 cttttaggta accctgcaat cggtgttaat ttcctgctgc ctatttgggc aatggcaaac
    31981 gttgcgcaag gcggtgcatg tctggcggta tggtttaaaa ccaaagatac aaaaattaaa
    32041 gcaatcaccc taccctcggc cttttccgca atgttaggga ttactgaagc cgctattttt
    32101 ggtatcaacc ttcgttttgt taaacctttt attgcggcct taattggtgg cgctgccggc
    32161 ggtgcctggg ttgtctctgt tcacgtttat atgactgccg ttggtctgac cgcaataccc
    32221 ggcatggcaa tcgttcagcc aacatcgttg gttaattata ttattggaat ggtgattgct
    32281 ttcgctgtcg cttttagcct ctctttatta ctcaaataca aaacggacga ggagtaatgt
    32341 cgatgccttc acaaatgccc gcgattttac aggcagtgat gaaaggtcag tccaaagcac
    32401 tggcagatgc ccactatcct tgttggcatc tcgctccagt caccggacta atgaacgatc
    32461 caaatggctt ttgttggtct ggaggccgct atcatctgtt ttatcaatgg aatccactgg
    32521 cttgtgatca taagtacaaa tgttgggggc actggagttc gacggattta ttgcactggc
    32581 agcatgaacc attagcgcta atgcccgata aagagtacga ccgtaatggt tgttactcag
    32641 gaagcgcggt taataatcag ggcgttctca cactttgcta taccggtaat gttaagtttg
    32701 atgatggcag cagaacagca tggcaatgtc tggccaccga aaataaccag ggcgggtttg
    32761 ataaactagg ccctgtcata ccgttacctg atggttacac cggacatgtt cgtgatccaa
    32821 aagtctggaa gcacaacagc caatggtaca tggtgcttgg cgcgcaagat aaagagaaac
    32881 gggggaaagt actgctgtat tcctctgtcg atctctatac ctggtctttt catggagaaa
    32941 ttgctggtaa tggtctgaat gaaatagata acgcaggata catgtgggaa tgtcctgatc
    33001 ttttcgccct tgatggtgaa tacattctcc tctgctgccc ccagggaatg gcgcgcgaac
    33061 atgagcgcta tctgaatact tatccctgcg cctggctaca tggacagttt gattacgaga
    33121 caggcaagtt tacgcatggc gctttctcag agctggatgc gggatttgaa ttctatgcac
    33181 cacagacgat ggaagccccg gacgggcgac gattgctggt cggctggatg ggggttcctg
    33241 atggcgaaga gatgttgcaa ccaaccagaa agcatcattg gcagcatcaa atgacctgtt
    33301 ttcgcgaact ctcattccag aagggaaaat tgtttcagat gccaatcagg gaactcgcgc
    33361 aactgcgtga agctgaacac ttctggcaag gaaaagcgga tcatgcgccg catgtcgaaa
    33421 ttgaacgcct ggaaatggac atcattccgt cgggtgagct gtatctgaat tttggcaatg
    33481 cgctggcact gcatcttaat gacgatggta ttcaactaca gaggagaagc cttgcagggc
    33541 aagagaaact aactcgttac tggcgaggaa gcgtaacgtc actgaagatt ctctgtgaca
    33601 gttccagcgt agagatattc atcaataatg gtgaaggtgt aatgagtaat cgttattttc
    33661 cccatcatcc agcttcgctg atattacagg gtgagtctga cgtcacgtta cgctactggt
    33721 cgctacgcgc ctgcatggta gaatgagggt tttgaattac ggatcctcgt cgtgagaaaa
    33781 accaaacgcg ttactattaa agacattgct gaactggcgg gagtgtcgaa agcaaccgcc
    33841 agtctggttc tcaatggtcg tggcaaagag ttacgtgttg cgcaggaaac gcgtgagcgt
    33901 gtgctgagta ttgcccaaca gcagcattat cagcccagca ttcatgcccg ctcattacgg
    33961 gacaaccgca gtcataccat tggtctcgtc gttcctgaaa tcactaacta cggatttgca
    34021 gtattttccc atgaactgga aacattgtgt cgggaagcgg gcgtccaact cctcatatcc
    34081 tgtactgatg aaaatccttc acaggaaaca atggtggtga acaatatgat tgcccgccag
    34141 gttgatgggt taatcgtcgc atccagtatg ctgcatgata atgactatca aaaacttagc
    34201 gagcaattac ctgttgtgtt gttcgatcgc cacatgaacg gcagcacatt gccattggtc
    34261 attactgact ctgtttctcc caccgccgct ctggtggcag atattgcacg gagtcacccg
    34321 gatgaattct attttctggg gggacaaccc cgtttgtcac cgactcgtga tcgactggaa
    34381 ggatttacac aaggcttgca gcaagccgga gtaacgctac aacctgaatg gattattcac
    34441 ggtaattatc atccgagctc aggctatgaa atgtttgccg cgctatgcgc tcgtttagga
    34501 cgtcccccta aagccttatt cactgccgct tgtggattac tggaaggcgt tctgcgctat
    34561 atgagccaat ataatttact cgacagtaaa atccacctgg cgagttttga tgatcattac
    34621 ttgtatgatt cactttcggt aagaatcgac acaatacaac aggataatcg ccaacttgct
    34681 tttcactgct ttgaacttat ttcgcaactg attgaaggtg aaacgccatc cccattacag
    34741 cgttatcttc ctgccagtct acaaaaacgc taccgataat cccgtcacta tcttgcatcg
    34801 cccctgtaaa acggggcgat tattttcatc ctgaacgtta aatatcaatg atgcataatc
    34861 aaatcaggca atatttaaat atttatgcgt ttgcatcgac aaacgccagt tgcgcgcaat
    34921 gcaggtatcg atacataaac gcgtagcatc ttccttctgg ctaattggct gcagtgcaat
    34981 gactcgcggt ttatcatcgg tcagcgtcgc aagcaattca tccagtgctt cgatatcacg
    35041 tacgcgtccc actgggtgtt tgatttcgtt ggctcgctcc agtgcctgtg acaacacttc
    35101 atagccaccg cgcatattca gctttggcga cacggtgacc caggtattcg gtgtacagcg
    35161 tacttcatga gtaccactgg tttctatctg gcagctaaaa ccgttctttt cgagcaggtc
    35221 agtcagcggc agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca cgtgacgcgc
    35281 ggtgtatccc tgacggccaa tgacagccag caaatcttca ctgctcgccg ccccccactt
    35341 atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat cctcaagctt
    35401 ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc ggacatccct gtaaacgaat
    35461 aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc agggtctgga acatctcgtt
    35521 aatcgggtac tgcatagcat tctctgtgaa gtggataatt gttaattatt gcagatcctg
    35581 ccacaacaat catgtcttat taacattctg ttacc
//
LOCUS       DACADF010000032        35287 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.032, whole genome shotgun sequence.
ACCESSION   DACADF010000032 DACADF010000000
VERSION     DACADF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.032"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            444..688
                     /locus_tag="ISB30_001529"
                     /pseudo
     CDS             444..688
                     /locus_tag="ISB30_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(705..1376)
                     /locus_tag="ISB30_001530"
     CDS             complement(705..1376)
                     /locus_tag="ISB30_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAP1989175.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYIFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLTSIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            1523..1798
                     /locus_tag="ISB30_001531"
     CDS             1523..1798
                     /locus_tag="ISB30_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1989176.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(1899..3485)
                     /gene="prpR"
                     /locus_tag="ISB30_001532"
     CDS             complement(1899..3485)
                     /gene="prpR"
                     /locus_tag="ISB30_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HAP1989177.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFSRHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKMSLAALSVPFSAALRQGLETCQILLLHYDWPGNIRE
                     LRNMMERLALFLSVESTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            3724..4614
                     /gene="prpB"
                     /locus_tag="ISB30_001533"
     CDS             3724..4614
                     /gene="prpB"
                     /locus_tag="ISB30_001533"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HAP1989178.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNILRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            4774..5943
                     /gene="prpC"
                     /locus_tag="ISB30_001534"
     CDS             4774..5943
                     /gene="prpC"
                     /locus_tag="ISB30_001534"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HAP1989179.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEIFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            5977..7428
                     /gene="prpD"
                     /locus_tag="ISB30_001535"
     CDS             5977..7428
                     /gene="prpD"
                     /locus_tag="ISB30_001535"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HAP1989180.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSRVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPVFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            7468..9354
                     /gene="prpE"
                     /locus_tag="ISB30_001536"
     CDS             7468..9354
                     /gene="prpE"
                     /locus_tag="ISB30_001536"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HAP1989181.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            9679..10938
                     /gene="codB"
                     /locus_tag="ISB30_001537"
     CDS             9679..10938
                     /gene="codB"
                     /locus_tag="ISB30_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HAP1989182.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            10928..12211
                     /locus_tag="ISB30_001538"
     CDS             10928..12211
                     /locus_tag="ISB30_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HAP1989183.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHFHDGKISAIDAQSGVMPVTE
                     NSLDAEQGLVLPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTKPAQTTVYLEQPEAIDYKR"
     gene            complement(12332..12943)
                     /gene="lacA"
                     /locus_tag="ISB30_001539"
     CDS             complement(12332..12943)
                     /gene="lacA"
                     /locus_tag="ISB30_001539"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HAP1989184.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEF
                     EKRESLIKEMFATVGENAWVDPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGCHVVINPGITIGDNSVIGA
                     GSVVTKDIPPNVVAAGVPCRVIREINDRDKQYYFKDFKVESSV"
     gene            complement(13009..14262)
                     /gene="lacY"
                     /locus_tag="ISB30_001540"
     CDS             complement(13009..14262)
                     /gene="lacY"
                     /locus_tag="ISB30_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HAP1989185.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSHFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLVAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(14314..17388)
                     /locus_tag="ISB30_001541"
     CDS             complement(14314..17388)
                     /locus_tag="ISB30_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HAP1989186.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFQVTTRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPELWSAEIPNLYRAVVKLHTADGTLIEAEACDVGFL
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQYFQFRLSGRTIEVTSEYLFRHSDNEFLHWMVALDGKPLASGEVPLDVGPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVVSVGVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFNRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(17511..18593)
                     /locus_tag="ISB30_001542"
     CDS             complement(17511..18593)
                     /locus_tag="ISB30_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP1989187.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPITEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(18670..19503)
                     /gene="mhpR"
                     /locus_tag="ISB30_001543"
     CDS             complement(18670..19503)
                     /gene="mhpR"
                     /locus_tag="ISB30_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HAP1989188.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLLEEGYVRRSTSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            19694..21358
                     /locus_tag="ISB30_001544"
     CDS             19694..21358
                     /locus_tag="ISB30_001544"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HAP1989189.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTEEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            21360..22304
                     /locus_tag="ISB30_001545"
     CDS             21360..22304
                     /locus_tag="ISB30_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HAP1989190.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            22322..23188
                     /locus_tag="ISB30_001546"
     CDS             22322..23188
                     /locus_tag="ISB30_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxononadienedioate/2-hydroxy-6-
                     oxononatrienedioate hydrolase"
                     /protein_id="HAP1989191.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            23198..24007
                     /gene="mhpD"
                     /locus_tag="ISB30_001547"
     CDS             23198..24007
                     /gene="mhpD"
                     /locus_tag="ISB30_001547"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HAP1989192.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            24004..24954
                     /locus_tag="ISB30_001548"
     CDS             24004..24954
                     /locus_tag="ISB30_001548"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HAP1989193.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVAQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            24951..25964
                     /gene="dmpG"
                     /locus_tag="ISB30_001549"
     CDS             24951..25964
                     /gene="dmpG"
                     /locus_tag="ISB30_001549"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HAP1989194.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGGDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            26140..27351
                     /gene="mhpT"
                     /locus_tag="ISB30_001550"
     CDS             26140..27351
                     /gene="mhpT"
                     /locus_tag="ISB30_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HAP1989195.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESTVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNSMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            27453..27992
                     /locus_tag="ISB30_001551"
     CDS             27453..27992
                     /locus_tag="ISB30_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HAP1989196.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQTQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(28117..28950)
                     /gene="fghA"
                     /locus_tag="ISB30_001552"
     CDS             complement(28117..28950)
                     /gene="fghA"
                     /locus_tag="ISB30_001552"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HAP1989197.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSIAGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(29043..30152)
                     /locus_tag="ISB30_001553"
     CDS             complement(29043..30152)
                     /locus_tag="ISB30_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HAP1989198.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(30187..30462)
                     /gene="frmR"
                     /locus_tag="ISB30_001554"
     CDS             complement(30187..30462)
                     /gene="frmR"
                     /locus_tag="ISB30_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HAP1989199.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            30768..31730
                     /gene="tauA"
                     /locus_tag="ISB30_001555"
     CDS             30768..31730
                     /gene="tauA"
                     /locus_tag="ISB30_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1989200.1"
                     /translation="MAISSRNTFLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            31743..32510
                     /gene="tauB"
                     /locus_tag="ISB30_001556"
     CDS             31743..32510
                     /gene="tauB"
                     /locus_tag="ISB30_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAP1989201.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            32507..33334
                     /gene="tauC"
                     /locus_tag="ISB30_001557"
     CDS             32507..33334
                     /gene="tauC"
                     /locus_tag="ISB30_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAP1989202.1"
                     /translation="MSVLINEILHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            33331..34182
                     /gene="tauD"
                     /locus_tag="ISB30_001558"
     CDS             33331..34182
                     /gene="tauD"
                     /locus_tag="ISB30_001558"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAP1989203.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(34222..35196)
                     /gene="hemB"
                     /locus_tag="ISB30_001559"
     CDS             complement(34222..35196)
                     /gene="hemB"
                     /locus_tag="ISB30_001559"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAP1989204.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         8126 a         9190 c         9784 g         8187 t
ORIGIN      
        1 atcagagctt tattatctgc gacgtttatt tttatttaag agagccacct gcaagttgtt
       61 attccaccag agagtgaatc gcaaagcagg tcatcatttc atctgtatat ctgtatgcca
      121 aatggcattc cccctccttt ttcgccgata ttttgttgtg atcgacgaag tttcgcttaa
      181 aaaagcacca gcagtggttt cgcagctata tggtgtataa aaaatgttct catacagatg
      241 ttttgtgaat gtgtgggttg acattcatat gaaaaaaatc ataattccat catgtttgtg
      301 tgggggcttt tctgtatctt acgcatcgca ctcaagcctg acagaaaata tgctgtaagg
      361 ctcatatcaa aaaccgccat tagctcatca ggaagagcag acgacaacca ggattgttgt
      421 atggtacggg gttcgaggcc tcgatggcgg tccaactttt caaaactctt ctgaatcaga
      481 tccccttatt atcatccctg caaagtggaa cattgccatt gtttggctat tcaggacggg
      541 tcagaccaat gaaaaagccc acacagggga gagtaggctg aaatgggaag ctaaagactc
      601 aagtaaactt atcggaaata aggaccacgc attacggggg ctatcatcgc cgatggttgg
      661 gattcggcaa atcaggttga ttacgtagag agtaaattat ctgctcaccg ctgcgtcacc
      721 ccttcgtaaa tcaggcgtag cgcgaataca ccaataattg caccaataac ccgactggca
      781 acgcgttgca tacgcccata agcacgacgc acagcgggca aagaaaacgc ctggcttaga
      841 aagactcgcc agataattga tgtgagcaca atccccgccc aggccattaa tcgtgcccat
      901 gttggtgttt cggcatttaa tgttactgag aaaatactga taaaaaataa aacggtttgc
      961 gggttagaga gatcggtaat taatccacgg cggaaaaaga tataccaggg ggcactaatc
     1021 ggttgttgta gtgtgctcat ttgcggtgtt gactggcggc gcatgctgca ccacgcaaac
     1081 cataagagat aagcgccgcc gacgattctg ataagcgaaa aaatctcctc acactgcgta
     1141 attagcgttg caagaccaaa caaacccaac ccggaataaa atgcatcgcc cagcgccacg
     1201 cccagcccgg tcaacacccc cgcgcgtcga ccggaagcca ggctggtttg tactaccaca
     1261 aagagattgg ctcccggatt aaaaaaagta atcacgaaca atcctacggt caggtaaacg
     1321 gcgtgcaaag gatccatagt gatttcatcc ataaataagt gaagtaactt catcatattt
     1381 ctaccaaaaa taatcgggtg cgagagagat cacaaagtgt cttatttccg gttactggcg
     1441 tttatgccct gactgaacta attattaatc aacccaataa tgtgggtggg tgatagtgtg
     1501 ataacaactc tggagccgta atatgaaaat aatctctaaa atgttagtcg gtgcgttagc
     1561 gtttgccgtt accaatgtct atgccgcaga attaatgacc aaagcggaat ttgaaaaggt
     1621 tgcatcgcaa tatgaaaaaa taggtgatat ttcaaccagt aatgaaatgt cgaccgcaga
     1681 tgcgaaagaa gatttgatca aaaaagccga tgaaaaaggg gctgatgtgt tggtactgac
     1741 ctctggtcaa actgacaata agatccacgg cacggcaaat atttataaga agaagtaatt
     1801 ctgaatccta tgtaaacatc cccgatgcgt tagtttatcg gtgatcatca tctatcgaaa
     1861 tttatgccgg ataaagcgtt cgcgctgcat tcggcagttc agcttttcag ccgccgccag
     1921 aacgtcgtcc ggctgatacc taaataattc gccgctgctg ttttatcgcc attaaatttc
     1981 tccagtgcct gttgtgctgt cagcaagcct ggaatgggag tcttcgccga ctcgcgcgcc
     2041 agttccggca gtagcagctg caaaaattgc ggcgttaaat ccggcgtcga ttccacactt
     2101 aaaaacagcg ccagtcgttc catcatattg cgcagttcac gaatattgcc cggccagtcg
     2161 tagtgcaata atagaatctg acaggtctct aatccctgac gtaatgcggc agaaaaaggg
     2221 acagagagtg ccgccagaga cattttcaaa aagctttccg ccagcggcag aatatccgcc
     2281 acccgctcgc gtagcggcgg cagttgtaga cgcaaaatac tcagccgata aaacaggtca
     2341 cggcgaaact gcccttgctg catatcttct tccagattgc agtgagtggc gctaatgacc
     2401 cgcacatcca ccggaacagg ctgatgcccg ccgacgcggg tgacctcttt ttcttccagc
     2461 acccgcagca gccgagtctg caacggcagc ggcatttcgc ctatctcgtc aagaaacagc
     2521 gtaccaccgt gggcgatttc aaacagcccg gcgcgtcctc cgcgtcgcga gccggtaaac
     2581 gccccttcct catagccaaa cagctccgct tccagcagcg attcggcaat cgccccgcag
     2641 ttaacggcaa caaacggatg tgactttttg ccctgtcgcg catcgtggcg ggaaaaatat
     2701 tcccgatgaa tcgcctgggc cgccagctct ttgcccgtcc ccgtttcccc ctcaatcaac
     2761 accgccgcac tggagcgggc atacagcaaa atagtctgcc gcacctgttc catctgtggt
     2821 gattgaccga gcatatcgcc cagcacgtaa cgagtacgca gggcgttgcg ggtggcatcg
     2881 tgagtgttat ggcgtaacga catgcgcgtc atatccagcg catcgctgaa tgcctggcgc
     2941 acggtggcgg cggaatagat aaaaattccg gtcattccgg cttcttctgc cagatcggta
     3001 atcagccctg cgccgaccac cgcttcggtg ccgttggctt tcaactcatt gatttgcccg
     3061 cgggcgtctt cttcggtgat gtagctacgt tggtcgaggc gcaaattaaa ggtcttttga
     3121 aacgccacca gcgctggaat ggtttcctga taggtgacaa caccgataga agaggtgagt
     3181 tttccggctt ttgccagtgc ctgtaacaca tcgtagccgc tcggtttaat caaaataact
     3241 ggcactgaca ggcgactttt aagatatgcg ccgttagagc cagcagcgat gatggcgtcg
     3301 caccgttcgt tggccagttt cttgcggatg taggtcaccg ctttttcaaa gccaagctgg
     3361 ataggggtga tattcgccag gtgatcaaac tcgaggctga tatcgcgaaa cagctcgaac
     3421 aggcgcgtta cagataccgt ccagataacc ggtttgtcgt cattcagccg tggtggatgt
     3481 gccatagcgc accgcaaagt taagaaaccg aatattgggt ttagtcttgt ttcataattg
     3541 ttgcaatgaa acgcggtgaa acattgcctg aaacgttaac tgaaacgcat atttgcggat
     3601 tacttcatga atttatcttt aactcattga aattaaatgt ttaattttat taaggcaatt
     3661 gtggcacacc cattgctttg tctttatcaa cgcaaataac aagttgataa caaaggatgg
     3721 gctatgtctc tacactctcc aggtaaagcg tttcgcgctg cactgactaa agaaaatcca
     3781 ttgcagattg ttggcactat caacgctaac catgcgctgc tggcgcagcg tgccggatat
     3841 caggcgattt atctctccgg cggtggcgtg gcggcagggt cgctggggct gcccgatctc
     3901 ggtatttcta ctcttgatga tgtgctgact gacattcgcc gtatcaccga cgtttgttcg
     3961 ctaccgctgc tggtggatgc ggatatcggt tttggttctt ctgcttttaa cgtggcgcgc
     4021 accgtgaaat cgatgattaa agccggtgcg gcaggattgc atattgaaga tcaggttggt
     4081 gcgaaacgct gcggtcatcg cccgaacaaa gcgatcgtct cgaaagagga aatggtggat
     4141 cggatccgtg cggcggtgga tgcgaaaacc gatcctgatt ttgtgatcat ggcgcgcacc
     4201 gatgcgctgg cggtagaggg gctggatgcg gcgatcgagc gtgcgcaggc ctatgttgaa
     4261 gcgggtgccg aaatgctgtt cccggaggcg attaccgaac tcgccatgta tcgccagttt
     4321 gccgatgcgg tgcaggtacc gatcctcgcc aacattaccg aatttggcgc aacaccgctg
     4381 tttaccaccg acgaattacg cagcgcccat gtcgcaatgg cgctctaccc gctttcagcg
     4441 tttcgcgcca tgaaccgcgc cgctgaacat gtctacaaca tcctgcgtca ggaaggcaca
     4501 cagaaaagcg tcatcgacac catgcagacc cgcaacgagc tgtacgaaag catcaactac
     4561 taccagtacg aagagaagct cgacgacctg tttgcccgtg gtcaggtgaa ataaaaacgc
     4621 ccgttggttg tattcgacaa ccgatgcctg atgcgccgct gacgcgactt atcaggccta
     4681 cgaggtgaac tgaactgtag gtcggataag acgcatagcg tcgcatccga caacaatctc
     4741 gaccctacaa atgataacaa tgacgaggac aatatgagcg acacaacgat cctgcaaaac
     4801 agtacccatg tcattaaacc gaaaaaatcg gtggcacttt ccggcgttcc ggcgggcaat
     4861 acggcgctct gcaccgtggg taaaagcggc aacgacctgc attaccgtgg ctacgatatt
     4921 cttgatctgg cggaacattg tgaatttgaa gaagtggcgc atctgctgat ccacggcaaa
     4981 ctgccaaccc gtgacgaact cgccgcctac aaaacgaaac tgaaagccct gcgtggttta
     5041 ccggctaacg tgcgtaccgt gctggaagcc ttaccggcgg cgtcacaccc gatggatgtt
     5101 atgcgcaccg gcgtttccgc gctcggctgc acgctgccag aaaaagaggg gcataccgtc
     5161 tctggcgcac gggatattgc cgacaaactg ctggcgtcac ttagctcgat tcttctctac
     5221 tggtatcact acagccacaa cggcgaacgc attcagccgg aaaccgatga cgactctatc
     5281 ggcggtcact tcctgcatct gctgcacggc gaaaagccgt cgcaaagctg ggaaaaggcg
     5341 atgcatattt cgctggtgct gtacgccgaa cacgagttta acgcttccac ctttaccagc
     5401 cgggtgattg cgggcactgg ctctgatatg tattccgcca ttattggcgc gattggcgca
     5461 ctgcgcgggc cgaaacacgg cggagcgaat gaagtgtcgc tggagatcca gcaacgctac
     5521 gaaacgccgg acgaagccga agctgatatc cgtaagcggg tggaaaacaa agaagtggtc
     5581 attggttttg ggcatccggt ttataccatc gccgacccgc gccaccaggt gatcaaacgc
     5641 gtggcaaagc agctctcgca ggaaggcggc tcgctgaaga tgtacaacat cgccgatcgc
     5701 ctggaaacag tgatgtggga gagcaaaaag atgttcccca atctcgactg gttctccgct
     5761 gtttcctaca acatgatggg tgttcccacc gagatattca caccactgtt tgttatcgcc
     5821 cgcgtcactg gctgggcggc gcacattatc gaacaacgtc aggacaacaa aattatccgt
     5881 ccttccgcca attatgttgg accggaagac cgccagtttg tcgcgctgga taagcgccag
     5941 taaacctcta cgaataacaa taaggaaacg tacccaatgt cagctcaaat caacaacatt
     6001 cgcccggaat ttgatcgtga aatcgttgat atcgtcgatt acgtgatgaa ctacgaaatc
     6061 agctccagag tagcctacga caccgcacat tactgcctgc tcgacacgct cggctgtggt
     6121 ctggaagctc tcgaatatcc ggcctgtaaa aaactgctgg ggccaattgt ccccggcacc
     6181 gtcgtaccca atggcgtgcg cgtccccgga actcagtttc agctcgaccc cgtccaggcg
     6241 gcatttaaca ttggcgcgat gatccgttgg ctcgatttca acgatacctg gctggcggcg
     6301 gagtgggggc atccttccga caacctcggc ggcattctgg caacggcgga ctggctttcg
     6361 cgcaacgcgg tcgccagcgg caaagcgccg ttgaccatga aacaggtgct gaccgcaatg
     6421 atcaaagccc atgaaattca gggctgcatc gcgctggaaa actcctttaa ccgcgtcggc
     6481 ctcgaccacg ttctgttagt gaaagtggct tccaccgccg tggtcgccga aatgctcggc
     6541 ctgacccgcg aggaaattct caacgccgtt tcgctggcat gggtagacgg acagtcgctg
     6601 cgcacctatc gccatgcgcc gaacaccggt acgcgtaaat cctgggcggc gggcgatgcc
     6661 acttcccgcg cggtacgtct ggcactgatg gcgaaaaccg gcgaaatggg ttatccgtca
     6721 gccctgactg cgccggtgtg gggcttctac gacgtctcct ttaaaggcga atcgttccgc
     6781 ttccagcgcc cgtatggttc ttacgtgatg gaaaatgtgc tgttcaaaat ctccttcccg
     6841 gcggagttcc actcccagac ggcagttgaa gcagcgatga cgctctatga acagatgcag
     6901 gcagcaggca aaacggcggc ggatatcgaa aaagtgacca ttcgcaccca cgaagcctgt
     6961 attcgcatca tcgacaaaaa agggccgctc aataacccgg ctgaccgcga ccactgcatt
     7021 cagtacatgg tggcgatccc gctgctgttc gggcgcttaa cggcggcaga ttacgaggac
     7081 aacgttgcgc aagataaacg catcgacgct ctgcgcgaga agatcaattg ctttgaagat
     7141 ccggtgttta ccgctgacta ccacgacccg gaaaaacgcg ccatcgccaa tgccataacc
     7201 cttgagttca ccgacggcac gcgatttgaa gaagtggtgg tggagtaccc aattggtcat
     7261 gctcgccgcc gtcaggatgg cattccgaag ctggtcgata aattcaaaat caatctcgcg
     7321 cgccagttcc cgactcgcca gcagcagcgc attctggagg tttctctcga cagaactcgc
     7381 ctggaacaga tgccggtcaa tgagtatctc gacctgtacg tcatttaagt aaacggcggt
     7441 aaggcgtaag ttcaacagga gagcattatg tcttttagcg aattttatca gcgttcgatt
     7501 aacgaaccgg agcagttctg ggccgagcag gcccggcgta ttgactggca gacgcccttt
     7561 acgcaaacgc tcgaccacag caacccgccg tttgcccgtt ggttttgtga aggccgaacc
     7621 aacttgtgtc acaacgctat cgaccgctgg ctggagaaac agccagaggc gctggcattg
     7681 attgccgtct cttcggaaac agaggaagag cgtaccttta ccttccgcca actgcatgac
     7741 gaagtgaatg cggtggcgtc aatgctgcgc tcgctgggcg tgcagcgtgg cgatcgggtg
     7801 ctggtgtata tgccgatgat tgccgaagcg catattaccc tgctggcctg cgcgcgcatt
     7861 ggtgctattc actcggtggt gtttgggggt tttgcctcgc acagcgtggc ggcgcgaatt
     7921 gatgacgcta aaccggtgct gattgtctcg gctgatgccg gggcgcgcgg cggtaaaatc
     7981 attccgtata aaaaattgct cgacgatgcg ataagtcagg cacagcatca gccgcgtcac
     8041 gttttactgg tggatcgcgg gctggcgaaa atggcgcgcg ttagcgggcg ggatgtcgat
     8101 ttcgcgtcgt tgcgccatca gcacatcggc gcgcgggtgc cggtggcatg gctggaatcc
     8161 aacgaaacct cctgcattct ctacacctcc ggcacgaccg gcaaacctaa aggtgtgcag
     8221 cgtgatgtcg gcggatatgc ggtggcgctg gcgacctcga tggacaccat ttttggcggc
     8281 aaagcgggcg gcgtgttctt ttgtgcttcg gatatcggct gggtggtagg gcattcgtat
     8341 atcgtttacg cgccgctgct ggcggggatg gcgactatcg tttacgaagg attgccgacc
     8401 tggccggact gcggcgtgtg gtggaaaatt gtcgagaaat atcaggttag ccgcatgttc
     8461 tcagcgccga ccgccattcg cgtgctgaaa aaattcccta ccgctgaaat tcgcaaacac
     8521 gatctttcgt cgctggaagt gctctatctg gctggagaac cgctggacga gccgaccgcc
     8581 agttgggtga gcaatacgct ggatgtgccg gtcatcgaca actactggca gaccgaatcc
     8641 ggctggccga ttatggcgat tgctcgcggt ctggatgaca gaccgacgcg tctgggaagc
     8701 cccggcgtgc cgatgtatgg ctataacgtg cagttgctca atgaagtcac cggcgaaccg
     8761 tgtggcgtca atgagaaagg gatgctggta gtggaggggc cattgccgcc aggctgtatt
     8821 cagaccatct ggggcgacga cgaccgcttt gtgaagacgt actggtcgct gttttcccgt
     8881 ccggtgtacg ccacttttga ctggggcatc cgcgatgctg acggttatca ctttattctc
     8941 gggcgcactg acgatgtgat taacgttgcc ggacatcggc tgggtacgcg tgagattgaa
     9001 gagagtatct ccagtcatcc gggcgttgcc gaagtggcgg tggttggggt gaaagatgcg
     9061 ctgaaagggc aggtggcggt ggcgtttgtc attccgaaag agagcgacag tctggaagac
     9121 cgtgaggtgg cgcactcgca agagaaggcg attatggcgc tggtggacag ccagattggc
     9181 aactttggcc gcccggcgca cgtctggttt gtctcgcaat tgccaaaaac gcgatccgga
     9241 aaaatgctgc gccgcacgat ccaggcgatt tgcgaaggac gcgatcctgg ggatctgacg
     9301 accattgatg atccggcgtc gttggatcag atccgccagg cgatggaaga gtaggtcgga
     9361 taaggcgctc gcgccgcatc cgacaccgtg cgcagatgcc tgatgcgacg ctgacgcgtc
     9421 ttatcatgcc tacaggcctg aaccgtaggt cggataaggc gctcgcgccg catccgacac
     9481 catgcgcaga tgcctgatgc gacgctgacg cgtcttatca tgcctaccca ctgtttttac
     9541 acagataagt ttcccccgcc tttttgtact cattcatata agaaatatat ccccatacga
     9601 aaacgattgc tttttatctt cagatgaata gaatgcggcg gattttttgg gtttcaaaca
     9661 gcaaaaaggg ggaatttcgt gtcgcaagat aacaacttta gccaggggcc agtcccgcag
     9721 tcggcgcgga aaggggtatt ggcattgacg ttcgtcatgc tgggattaac cttcttttcc
     9781 gccagtatgt ggaccggcgg cactctcgga accggtctta gctatcatga tttcttcctc
     9841 gcagttctca tcggtaatct tctcctcggt atttacactt catttcttgg ttacattggc
     9901 gcaaaaaccg gcctgaccac ccatcttctt gctcgcttct cgtttggtgt taaaggctca
     9961 tggttgcctt cactgctgct gggcggcacc caggttggct ggtttggcgt tggcgtggcg
    10021 atgtttgcta ttccggtggg caaggcaacc gggctggata ttaatttgct gattgccgtc
    10081 tccggtttac tgatgaccgt caccgtcttc tttggcattt cggcgctgac agttctttcg
    10141 gtgattgcgg ttccggctat cgcctgtctt ggcggttatt ccgtgtggct ggccgttaac
    10201 ggcatgggcg gcctggacgc attaaaagcg gtcgttcccg cacaaccgtt agatttcaat
    10261 gtcgcgctgg cgctggttgt ggggtcattt atcagtgcgg gcacgctcac cgctgacttt
    10321 gtccggtttg gtcgtaatgc caaactggcg gtgctggtgg cgatggtggc ctttttcctc
    10381 ggcaactcgt tgatgtttat tttcggtgca gcgggcgcgg cggcactggg gatggcggat
    10441 atctctgatg tgatgattgc tcagggcctg ctgctgcctg cgattgtggt gctggggctg
    10501 aatatctgga ccaccaacga taacgcgctc tatgcgtcgg gtttaggttt cgccaacatt
    10561 accggtatgt cgagcaaaac cctttcggtg attaacggta ttatcggtac ggtctgcgca
    10621 ttatggctgt ataacaattt tgtcggctgg ttgaccttcc tttcggcagc tattcctccg
    10681 gtgggcggcg tgatcatcgc tgattatctg atgaaccgtc gccgctatga gcactttgcg
    10741 accacgcgta tgatgagtgt caattgggtg gcgattctgg ctgtcgcgct ggggattgcc
    10801 gcaggccact ggttaccggg aattgttccg gtcaacgcgg tattaggtgg cgcgctgagc
    10861 tatctgatcc ttaacccgat tttgaatcgt aaaacgacag cagcaatgac gcatgtggag
    10921 gctaacagtg tcgaataatg ctttacaaac aattattaac gcccggttac ccggcaaaga
    10981 ggggctgtgg cagattcatt ttcacgacgg aaaaatcagc gccattgatg cgcaatccgg
    11041 cgtgatgccc gtaactgaaa acagcctgga tgccgaacaa ggtttagttt taccgccgtt
    11101 tgtggagccg catattcacc tggacaccac gcaaaccgcc ggacaaccga actggaatca
    11161 gtccggcacg ctgtttgaag gcattgaacg ctgggccgag cgcaaagcgt tattaactca
    11221 tgacgatgtg aaacaacgcg catggcaaac gctgaaatgg cagattgcca acggcattca
    11281 gcatgtgcgt acccatgtcg atgtttcgga cgcaacgctg actgcgctga aagcaatgct
    11341 ggaagtgaaa caggaagttg cgccgtggat tgatctacaa atcgtcgcct tcccgcagga
    11401 agggattttg tcgtatccca atggtgaagc gttgctggaa gaggcgttac gcttaggggc
    11461 agacgttgtt ggggcgattc cgcattttga atttacccgt gaatatggcg tggagtcgct
    11521 gcataaaacc ttcgccctgg cgcaaaaata cgaccgtctc atcgatgttc actgtgacga
    11581 gattgatgat gagcaatcac gctttgtcga aaccgttgct gccctggcgc accgtgaagg
    11641 catgggcgcg cgagtcaccg ccagccatac tacggcaatg cactcctata acggggcgta
    11701 tacttcgcgc ctgtttcgtt tgctgaaaat gtccggtatt aactttgtcg ccaacccgtt
    11761 ggtcaatatt catctgcaag gacgtttcga tacgtatcca aaacgtcgcg gcatcacgcg
    11821 cgttaaagag atgctggagt ccggcattaa cgtctgcttt ggtcacgatg atgtcttcga
    11881 tccgtggtat ccgctgggaa cggcgaatat gctgcaagtg ctgcatatgg ggctgcatgt
    11941 ttgccagctg atgggctatg ggcagattaa cgacggcctg aatttaatca cccaccacag
    12001 tgccagaacg ttgaatttgc aggattacgg cattgccgcc ggaaacagcg ccaacctgat
    12061 tatcctgccg gctgaaaatg ggtttgacgc gctgcgccgt caggttccgg tgcgctattc
    12121 ggtacgtggc ggcaaggtga ttgccagcac aaaaccggca caaaccaccg tatatctgga
    12181 gcagccagaa gccatcgatt acaaacggta atcggctggg ttaccgcgag cgtagtttat
    12241 gccggatgcg gcgtgaacac cttatccggc ctacgtagat cactgaaatt gtaggcctga
    12301 taagcggagc gcatcaggca attttgctta tttaaactga agactcaact ttaaaatctt
    12361 tgaaataata ttgcttatcc cggtcgttta tttcgcgaat aacccgacaa ggaacgcctg
    12421 cagccacgac gtttggtgga atgtcttttg tgacgacact acccgcgcca ataacagaat
    12481 tatccccgat ggtgatgcct ggattaataa ccacatgaca tccgatccag acgttattgc
    12541 caatcgttat cggaaaagag tacatctctc cgttttttct caattcatgg tgtaccgggt
    12601 gtcccgtaac ggaaagagta acgttgggtg caatcagtac gttatcaccg atcgttaccg
    12661 tgtagtcgtc gacaatggtt aaattgaaat ttgcataaaa attgcggcct atatggatgt
    12721 tggaaccgta agagaaatag acgggggggt ctacccaggc gttttcccct accgtggcaa
    12781 acatttcttt aatcagactt tccctttttt caaattctga tggatgcgag tgattaaact
    12841 catacattaa cgtttttcca cgaagtcttt tttcaggtaa gccttcgcac atatcggtaa
    12901 atagcttgcc tgcttttatt ctttctgtca tcgacatgtt cattgcgatc attccgccct
    12961 gatatgttgg tcggataagg cgctcgcgcc gcatccgaca ttgattgctt aagcgacttc
    13021 attcacctga cgacgcagta gagaaagcgg gccggggccg ctaagcgtga acacggaaat
    13081 taaggtgaag cccagcgcca ccagacccag caccagataa gcgccctgga aaccgatgct
    13141 ttcatacata ttgcccgcca gtacagacat aaaaatcatc gccagttgct taaagaagca
    13201 gaaacagacc agataaatcg tcgctgaaaa acgcacttca aactggctgg taatatattt
    13261 aaagcagccc accagcagga acggtacttc aaacatatgc agcgttttca gaataaccac
    13321 ttccagcgct gaggtggcga acgatgagcc aataatacgt acagacataa tagtgccagc
    13381 caccagcagg gcatttttcc caccgatgcg attaatgatc agtggcgcaa agaacataat
    13441 cgaggcatta agtaattcgc ccattgtcgt tacgtagcca aatacccgcg taccctgttc
    13501 accggtagca aaaaacgaag taaagaaatt agcaaactgt tggtcaaaaa catcgtaggt
    13561 gcaggaaacg ccaataacat acagtgacaa aaaccacagt tttggctgtc tgaacagttc
    13621 cagcgccagc ttaaggctaa atgccgaatg gttggcacct accgcattgg caaccgtggc
    13681 tgaagagggc gcatccgttt tggcgaaaaa gagtaaaacg gcgaggatga gtgcacagcc
    13741 agaacccagc cagaaaacaa actgattatt gatggtgaac atgatgccga caatcgaggc
    13801 acacagcgcc cagccaacac agccaaacat ccgcgcgcga ccaaattcga aatgactgcg
    13861 acggctgact ttctcgataa atgcctctac tgctggcgca ccggcgttaa aacaaaagcc
    13921 aagataaata ccaccaacaa tcgatcctac taaaatgttg tattgtaaca gtggtccgaa
    13981 gataaaaata aagaacggcg caaacatcac taacatgccg gtaataatcc acagcaggta
    14041 tttgcgcagc ccgagtttgt cagaaagcag accaaacagc ggttggaata atagcgagaa
    14101 cagagaaata gcggcaaaaa taatacccgt atcacttttg ctgatatggt tgatgtcatg
    14161 tagccaaatc gggaaaaacg ggaagtaggc tcccatgata aaaaagtaaa agaaaaagaa
    14221 taaaccgaac atccaaaagt ttgtgttttt taaatagtac ataatggatt tccttacgcg
    14281 aaatacgggc agacatggcc tgcccggtta ttattatttt tgacaccaga ccaactggta
    14341 atggtagcga ccggcgctca gctggaattc cgccgatact gacgggctcc aggagtcgtc
    14401 gccaccaatc cccatatgga aaccgtcgat attcagccat gtgccttctt ccgcgtgcag
    14461 cagatggcga tggctggttt ccatcagttg ttgttggctg tagcggctga tgttgaactg
    14521 gaagtcgccg cgccactggt gtgggccata attcaattcg cgcgtcccgc agcgcagacc
    14581 gttttcgctc gggaagacgt acggggtata catgtctgac aatggcagat cccagcggtt
    14641 aaaacaggct gcagtaaggc ggtcgggata gttttcttgc ggccccaggc cgagccagtt
    14701 tacccgctct gcgacctgcg ccagctggca ggtcaggcca atccgcgccg gatgcggtgt
    14761 atcgctcgct acttcaacac ctacactgac gaccatctcg ccgtgtccat caatccggta
    14821 ggttttccgg ctgataaata aggttttccc ctgatgctgc cacgcgtggg cggtcgtaat
    14881 cagcaccgcg tcggcaagtg tatctgccgt gcactgcaac aacgccgctt cggcctggta
    14941 atggccagcc gccttccagc gttcgaccca ggcgttaggg tcaatgcggg tcgcttcact
    15001 tacgccaatg tcgttatcca gcggcgcacg ggtgaactga tcgcgcagcg gggtcagcag
    15061 ttgtttttca tcgccaatcc acatctgtga aagaaagcct gactggcggt taaattgcca
    15121 acgcttatta cccagctcga tgcaaaaatc cgtttcgctg gtggtcagtt gagggatggc
    15181 gtgggacgcg gaggggagtg tcacgctgag gttttccgcc agacgccatt gctgccaggc
    15241 gctgatgtgt ccggcttctg accatgcggt cgcgtttggt tgcactacgc gtaccgttag
    15301 ccagagttgt ccggcgctct ccggctgcgg cagttcaggc agttcaatca actgcttacc
    15361 ttgcgggcca acatccagag gcacttcacc gcttgccagc ggcttgccat ccagcgccac
    15421 catccagtgc aggaactcgt tatcgctatg acggaacagg tattcgctgg tcacttcgat
    15481 ggttcgcccg gataaacgga actggaaata ctgctgttgg tgttttgctt ccgtcagcgc
    15541 cggatgcggc gtgcggtcgg caaagaccag accgttcata cagaactggc gatcgttcgg
    15601 cgtatcgcca aaatcaccgc cgtaagccga ccacgggttg ccgttttcat catatttaat
    15661 cagcgactga tccacccagt cccagacgaa gccgccctgt aaacgggggt actgacgaaa
    15721 cgcctgccag tatttagcga agccgccaag actgttaccc atcgcgtggg catattcgca
    15781 aaggatcagc gggcgcattt ctccaggcag cgaaagccat tttttgatgg accatttcgg
    15841 caccgccggg aagggctggt cttcatccac gcgcgcgtac atcgggcaaa taatatcggt
    15901 tgcggaggtg tcggctccgc cgccttcata ctgtaccggg cgggaaggat cgacagattt
    15961 gatccagcga tagagtgcgt cgtgattagc gccgtggcct gactcattcc ccagcgacca
    16021 gatgatcaca ctcgggtgat tacgatcgcg ctgcaccatc cgcgttacgc gttcgctcat
    16081 cgcgggtagc cagcgcggat catcggtcag acgattcatt ggcaccatgc cgtgggtttc
    16141 aatattggct tcatccacca catacaggcc gtaacggtcg cacagcgtgt accacagcgg
    16201 atggttcgga taatgcgaac agcgcacggc attaaagttg ttctgcttca tcagcaggat
    16261 atcctgcacc atcgtctgct catccatgac ctgaccatgc agaggatgat gctcgtgacg
    16321 gttaacgccg cgaatcagca acggcttgcc gttcagcagc agcagaccat tttcaatccg
    16381 cacctcgagg aaaccgacat cgcaggcttc tgcttcaatc agcgtgccgt cggcggtgtg
    16441 cagtttaacc accgcacgat agagattcgg gatttcggcg ctccacagtt ccggattttc
    16501 aacgttcagg cgtagcgtga cgcgatcggc ataaccgcca cgctcatcga taatttcacc
    16561 gccgaaaggc gcggtgccgc tggcgacctg cgtttcaccc tgccacaaag aaaccgtcac
    16621 ccgcagttca tcgcgcagct cgccgtacat ctgaacttct gcctccagta ccgcgcggct
    16681 gaaatcatca ttaaagcgag tggtaacttg gaaatcgctg atttgcgtgg tcggtttatg
    16741 cagcaacgag acgtcacgga aaatgccgct catccgccac atatcctgat cttccagata
    16801 actgccgtca ctccagcgca gcaccatcac cgcgaggcgg ttttctccgg cgcgtaaaaa
    16861 tgcgctcagg tcaaattcag acggcaaacg gctgtcctgg ccgtaaccga cccagcgccc
    16921 gttgcaccac agatgaaacg ccgagttaac gccatcaaaa ataattcgcg tctggccttc
    16981 ctgtagccag ctttcatcaa cattaaatgt gagcgagtaa caacctgtcg gattctccgc
    17041 gggaacaaac ggcggattga ccgtaatggg ataggttacg ttggtgtaga tgggcgcgtc
    17101 gtaaccgtgc atctgccagt ttgaggggac gacgacagta tcggcgtcag gaagatcgca
    17161 ctccagccag ctttccggca ccgcttctgg tgccggaaac caggcaaagc gccattcgcc
    17221 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tatttcgcca
    17281 gctggcgaaa gggggatgtg ccgcaaggcg attaagttgt gtaacgccag ggttttccca
    17341 gtcacgacgt tgtaatacga cggccagaga atccgtaatc atagtcatag ctgtatcctg
    17401 tgtgaaattg ttatccgctc acaatttcac acaacatacg agccggacac atagagtgta
    17461 aagactgggg tgcctaatga gtgagctgac tcacattaat tgtgttgcgc tcactgcccg
    17521 ctttccagtc gggaaacttg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    17581 gaggcggttt gcgtattggg cggaagggtg gtttttcttt tcaccagtga gacgggcaac
    17641 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
    17701 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acacgagctg
    17761 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg
    17821 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
    17881 acgatgccct catttagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
    17941 ccttcccgtt ctgttatcgg ctgaatttga ttgcgtgtga gatatttatg ccagcccgcc
    18001 agacgcagac gcgccgagac agaacttaat gggcctgcta acagcgcgat ttgctggtga
    18061 cccaatgcga ccaggtgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
    18121 ctgttgatgg gagtctggtc agagacatca agaaataacg ccggaacatt agcgcaggca
    18181 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
    18241 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
    18301 atcgacacca ccacgctggc acccagttga tcggcgcgag attttatcgc cgcaacaatt
    18361 tgcgacggcg cgtgcagggc cagactggaa gtggcaacgc caatcagcaa cgactgtttg
    18421 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
    18481 actttttccc gcgttttcgc tgaaacgtgg caggcctggt tcaccacgcg ggaaacggtc
    18541 tgataagaga caccggcata ctctgcgaca tcgtatagcg ttactggttt cacattcacc
    18601 accctgaatt gactctcttc cgggcgctat catgccatac cacgaaaggt tttgcgccat
    18661 tcgatggtgt cagcgtaaat gcacgccgct tcgccttccc gctaccagaa tagcctgcga
    18721 ttcaacccct tcttcgatct gttttgccac ccgttgcagc gccggaagat gcttttccgc
    18781 tgcctgttcg atggtcattg cgctcgccat atacaccaga ttcagacagc caatcacccg
    18841 ttgttcactg cgcagcggta cggcgataga ggcgatcttc tcctcctgat cccagccgcg
    18901 gtagttctgt ccgtacccct ctttgcgcgc gcgcgccaga atggcttcca gctttaacgg
    18961 ttctcgtgcc agttgatagt catcaccggg gcgggaggct aacatctcga ttaattcctt
    19021 gcgttcttgt tccgggcaaa aggccagcca ggtcaggccc gaggcggttt tcagaagcgg
    19081 taaccgtcgc ccgaccattg cccggtgaaa agataagcgg ctgaaacgat gggtggtttc
    19141 gcgtaccacc attgcatcaa catccagcgt ggacacatct gtcggccata ccacttcgcg
    19201 caacagatcg cccagcagcg gggccgccag tgcagaaatc cactgttcgt cacgaaatcc
    19261 ttcgcttaat tgccgcactt tgatggtcag acgaaaactg tcatcggagg tactgcgccg
    19321 gacatatccc tcttccagta acgtctccag cagtcgccgc acagtggtgc gatgcaggcc
    19381 gctgagttcc gccagcagcc cgacgctggc accgccatca agtttattta acatatttaa
    19441 taacattaga ccgcgggtta agccgcgcac ggttttgtat tccgtctgct cattgttctg
    19501 catattaatt gacatttata tagttaaaac aacgttgtgc acctggtgca cattcgggca
    19561 tgttttgatt gtagccgaaa acacccttcc tatactgagc gcacaataaa aaatcattta
    19621 catgttttta acaaaataag ttgcgctgta ctgtgcgcgc aacggcattt tgtccgagtc
    19681 gtgaggtact gaaatggcaa tacaacaccc tgacatccag cctgctgtta accatagcgt
    19741 tcaggtggcg atcgctggtg ccggtccggt cgggctgatg atggcgaact atctcggcca
    19801 gatgggcatt gacgtgctgg tggtggagaa actcgataag ttgatcgact acccgcgtgc
    19861 gattggtatt gatgacgagg cgctgcgcac catgcagtcg gttggcctgg tcgataatgt
    19921 tctgccgcac actacgccgt ggcacgcgat gcgttttctc accccaaaag gtcgctgttt
    19981 tgctgatatt cagccaatga ccgatgaatt tggctggccg cgccgtaacg cctttattca
    20041 gcctcaggtc gatgcggtga tgctggaagg attgtcgcgt tttccgaatg tgcgctgcct
    20101 gttttcccgc gagctggagg ccttcagcca gcaagatgac gaagtgacct tgcacctgaa
    20161 aacggaagaa gggcagcggg aaacggtcaa agcccagtgg ctggtggcct gtgatggtgg
    20221 ggcaagtttt gtccgtcgca ccctgaatgt gccgtttgaa ggtaaaactg cgccaaatca
    20281 gtggattgtg gtagatatcg ccaacgatcc gttaagtacg ccgcatatct atttgtgttg
    20341 tgatccggtg cgcccgtatg tttctgccgc gctgcctcat gcggtacgtc gctttgaatt
    20401 tatggtgatg ccgggagaaa ccgaagaaca gctgcgtgag ccgcaaaata tgcgcaagct
    20461 gttaagcaaa gtgctgccta atccggacaa tgttgaattg attcgccagc gtgtctacac
    20521 ccacaacgcg cgactggcgc aacgttttcg tattgatcgc gtactgctgg cgggcgatgc
    20581 cgcgcacatc atgccggtgt ggcaggggca gggctataac agtggtatgc gcgacgcctt
    20641 taacctcgca tggaaactgg cgttggttat ccaggggaaa gcccgcgatg cgctgctcga
    20701 tacctatcaa caagaacgtc gcgatcacgc caaagcgatg attgacctgt ccgtgacggc
    20761 gggcaacgtg ctggctccgc cgaaacgctg gcagggtacg ttacgtgacg gcgtttcctg
    20821 gctgctgaat tatctgccgc cagtaaaacg ctacttcctc gaaatgcgct tcaagccgat
    20881 gccgcaatat tacggcggtg cgctggtgcg tgagggcgaa gcgaagcact ctccggtcgg
    20941 caagatgttt attcagccga aagtcacgct ggaaaacggc gacgtgacgc tactcgataa
    21001 cgcgatcggc gcgaacttcg cggtaattgg ctggggatgc aatccactgt gggggatgag
    21061 cgacgagcaa atccagcagt ggcgcgcgtt gggcacacgc ttcattcagg tggtgccgga
    21121 agtgcaaatt cataccgcac aggataacca cgacggcgta ctacgcgtgg gggatacgca
    21181 aggtcgcctg cgtagctggt tcgcgcaaca caatgcttcg ctggtggtga tgcgcccgga
    21241 tcgctttgtt gccgccaccg ccattccgca aaccctgggc aagaccctga ataaactggc
    21301 gtcggtgatg acgctgaccc gccctgatgc cgacgtttct gtcgaaaagg tagcctgata
    21361 tgcacgctta tcttcactgt ctttcccact cgccgctggt ggggtatgtc gacccggcac
    21421 aagaggtact cgatgaggtc aatggcgtga ttgccagcgc ccgcgagcgt attgcggctt
    21481 tctcccctga actggtggtg ctgtttgcgc cagatcacta caacggcttt ttctatgacg
    21541 tgatgccacc gttctgttta ggcgttggag cgacggcaat tggtgatttc ggcagtgcgg
    21601 caggagagct gcccgtgcct gtggagctgg cggaggcctg tgcgcatgcc gtcatgaaga
    21661 gcgggatcga tcttgccgtt tcttactgta tgcaggtgga ccacgggttc gcccagccgc
    21721 tggagttcct gctcggtggg ctggataagg tgccagtact gccggtgttc atcaacggtg
    21781 tcgccacgcc gctgcccggt ttccagcgta cccgcatgtt gggggaagcc attggacgtt
    21841 tcaccagtac tctcaataaa cgcgtgctgt tcctgggttc cggtgggctt tcccatcagc
    21901 cgccggtgcc cgaactggcg aaagccgatg cccatatgcg cgaccgtctg ttggggagcg
    21961 ggaaagattt acccgccagt gagcgcgaat tgcgtcagca acgggtgatt agcgccgctg
    22021 agaagtttgt tgaggatcag agaacgctgc atccgctcaa cccgatttgg gataaccagt
    22081 tcatgacttt gctggagcag ggacgcatac aggaactgga tgccgtcagt aacgaagagc
    22141 tttccgccat tgccggaaag tcgacacatg aaatcaaaac ctgggtcgcc gcttttgccg
    22201 ctatttctgc gtttggcaac tggcgtagcg aagggcgtta ttaccgccca atcccggagt
    22261 ggattgccgg atttggctcg ttaagcgcca gaacagagaa ctgaatatgc aggagaagat
    22321 gatgagttat cagccacaaa ccgaagccgc caccagccgt tttctgaatg tagaagaagc
    22381 gggtaaaacg ctgcgcatcc attttaatga ctgcggacaa ggcgacgaaa ccgttgtcct
    22441 gctgcatggt tccggcccgg gtgctactgg ctgggcgaac ttcagccgca atatcgatcc
    22501 gctggtagag gcgggctatc gggtgatcct gctggattgt ccgggttggg gtaagagcga
    22561 ttcgatcgtt aatagtggtt cgcgatcgga tcttaatgcg cgaatcctga aaagcgtggt
    22621 ggatcaactg gatatcgcca aaatccacct gctgggcaac tcgatgggtg gccacagttc
    22681 tgtggcgttc actcttaact ggccggagcg cgtcggcaaa ctggtgctga tgggcggcgg
    22741 tacgggcggc atgagtttgt ttacgccgat gccaaccgaa ggtattaagc gactgaatca
    22801 gctttatcgt cagccgacta tcgaaaacct gaagctgatg atggatatct tcgtttttga
    22861 taccagcgat ttgaccgacg ccctgtttga agcgcgcctg aataatatgc tgtcgcgccg
    22921 cgatcacctg gaaaacttcg ttaagagcct ggaagccaat ccgaaacagt tcccggattt
    22981 tggcccacgt ctggcggaaa tcaaagcgca aaccctgatt gtctgggggc gcaacgaccg
    23041 ctttgtgccg atggatgcgg gtctgcgtct gctgtccggc attgccggtt ctgaactgca
    23101 tatcttccgc gactgtggtc actgggcgca gtgggaacat gccgacgctt tcaatcaact
    23161 ggtgctgaat ttcctcgcac gcccttaagg aacggtcatg acgaagcata ctcttgagca
    23221 actggcggcg gatttacgcc gcgccgcaga gcagggcgaa gcgattgcgc cgctgcgcga
    23281 tctgattggt atcgataacg ctgaagcggc ctacgccatt cagcacatca atgtgcaaca
    23341 tgacgttgcg caggggcgtc gcgtggtggg gcgcaaagtg gggctgacgc atccgaaagt
    23401 gcagcaacaa ctgggcgttg atcaaccgga tttcgggaca ttatttgccg acatgtgtta
    23461 tggcgataat gaaaccattc ctttttcccg tgttctgcaa ccccgcattg aagcggagat
    23521 tgcactggtg ttgaaccgcg atctacccgc aaccgatatc accttcgacg aattgtataa
    23581 cgccattgaa tgggtacttc cggcgctgga ggtggtgggg agccgcattc gcgactggtc
    23641 gattcagttt gtcgataccg tggcagataa cgcttcctgc ggggtgtatg tcatcggcgg
    23701 tccggcgcaa cgcccggcgg ggttagacct gaaaaactgc gccatgaaga tgacgcgtaa
    23761 taacgaagag gtttccagcg ggcgcggcag cgaatgcctg ggacatccgc ttaatgccgc
    23821 cgtctggctg gcacgcaaaa tggccagtct gggtgaaccg ctgcgcgccg gagatatcat
    23881 tcttaccggg gcattaggtc cgatggtggc ggtgaatgcg ggcgatcgtt ttgaagccca
    23941 tatcgaaggc ataggttcag ttgctgcgac attttcaagc gcagccccaa aaggaagtct
    24001 gtcatgagta agcgtaaagt cgccattatc ggttcaggca acattggcac cgatctgatg
    24061 attaaaattt tgcgtcacgg tcagcatctg gagatggcgg tgatggtcgg cattgatcct
    24121 cagtccgacg gtctggcgcg tgccagacgt atgggcgtcg ccaccaccca tgaaggggtg
    24181 atcgggctga tgaacatgcc tgaatttgct gatatcgaca ttgtatttga tgcgaccagc
    24241 gcgggcgcgc atgtgaaaaa cgatgccgct ttgcgcgaag cgaaaccgga tattcgctta
    24301 attgacctga cgcctgctgc catcggccct tactgcgtgc cggtggttaa cctcgaggag
    24361 aacgtcgctc aactgaacgt caacatggtc acctgcggcg gccaggccac cattccaatg
    24421 gtagcggcag tttcacgcgt ggcgcgtgtt cattacgccg aaattatcgc ttctattgcc
    24481 agtaaatctg ccggacccgg cacgcgtgcc aacatcgatg aatttacgga aaccacttcg
    24541 cgcgccattg aagtggtggg tggcgcggca aaagggaagg cgattattgt gcttaaccca
    24601 gcagagccac cgttgatgat gcgtgacacg gtgtatgtat tgagcgacga agcttcacaa
    24661 gatgatatcg aagcctcaat caatgaaatg gctgaggcgg tgcaggctta cgtaccgggt
    24721 tatcgcctga aacagcgcgt gcagtttgaa gtcatcccgc aggataaacc ggtcaattta
    24781 ccgggcgtgg ggcagttctc cgggctgaaa acggcggtct ggctggaagt cgaaggcgca
    24841 gcgcattatc tgcctgccta tgcgggcaac ctcgacatta tgacttccag tgcgctggcg
    24901 acagcggaaa aaatggccca gtcactggcg cgcaaggcag gagaagcggc atgaacggta
    24961 aaaaacttta tatttcggac gtcacgttgc gtgacggtat gcacgccatt cgtcatcagt
    25021 attcgctgga aaatgtccgc cagattgcca aagcactgga tgatgcccgc gtggattcga
    25081 ttgaagtggc ccacggcgac ggtttgcagg gttccagctt taactatggt ttcggcgcac
    25141 atagcgacct tgaatggatt gaagcggcgg cggatgtggt gaagcacgcc aaaatcgcga
    25201 cgttgttgct gccaggaatc ggcactattc acgatctgaa aaatgcctgg caggctggcg
    25261 cgcgggtggt tcgtgtggca acgcactgta ccgaggctga tgtttccgcc cagcatattc
    25321 agtatgcccg cgagctcgga atggacaccg ttggttttct gatgatgagc catatgacca
    25381 cgccggagaa tctcgcaaag caggcaaagc tgatggaagg ctacggtgcg acctgtattt
    25441 atgtggtgga ttctggcggt gcgatgaaca tgagcgatat ccgtgaccgt ttccgcgccc
    25501 tgaaagcagt gctgaaacca gaaacgcaaa ccggcatgca cgctcaccat aacctgagtc
    25561 ttggcgtggc gaattctatc gcggcggtgg aagagggctg cgaccgaatc gacgccagcc
    25621 tcgcgggaat gggcgcgggc gcaggtaacg cgccgctgga agtatttatt gccgccgcgg
    25681 ataaactggg ttggcagcat gggggcgatc tctatgcgtt aatggatgcc gctgacgacc
    25741 tggtgcgtcc gttgcaggat cgaccggtac gagtcgatcg cgaaacgctg gcgctgggat
    25801 acgctggtgt ttactccagc ttcctgcgtc actgtgaaac ggcggcggcg cgttatggct
    25861 taagcgcggt ggatattctc gttgagctgg gcaaacgccg gatggttggc ggccaggagg
    25921 atatgatcgt cgacgtggcg ctggatctgc gcaacaacaa ataatattga ctgccgagaa
    25981 cgtgcatttt gtatgcccat cccagcacga ctgccggatg cggcgtgaac gccttatccg
    26041 gcctacattt cgcccgtagg cagtcattaa atagttctga ttacgcggct ggcaatgtat
    26101 cagtcgcgat tcacctgcac tcgcaatgag gttcatctta tgtcgactcg taccccttca
    26161 tcatcttcat cccgcctgat gctgaccatc gggctttgtt ttttggtcgc tctgatggaa
    26221 gggctggatc ttcaggcggc tggcattgcg gcgggtggca tcgcccaggc tttcgcactc
    26281 gataaaatgc aaatgggctg gatatttagc gccggaatac tcggtttgct acccggcgcg
    26341 ctggttggcg ggatgctggc agaccgttat gggcgtaagc gtattttgat tggctcagtt
    26401 gcgctgtttg gtttgttctc gctggcaacg gcgattgcat gggatttccc ctcactggtc
    26461 tttgcgcggc tgatgaccgg tgtcgggctg ggggcggcgt tgccgaatct tatcgccctg
    26521 acgtctgaag ccgcgggacc acgttttcgt gggacggcag tgagcctgat gtattgcggt
    26581 gttcccattg gcgcggcgct ggcggcgacg ctgggtttcg cgggggcaaa cttagcatgg
    26641 caaacggtgt tttgggtagg tggtgtggtg ccgttgattc tggtgccgct attaatgcgc
    26701 tggctgccgg agtcgacggt tttcgctggc gaaaaacagg ctgcgccacc actgcgtgcg
    26761 ttatttgcgc cagaaacggc aaccgcgacg ctgctgctgt ggttgtgtta ttttttcact
    26821 ctgctggtgg tctacatgtt gatcaactgg ctgccgctgc ttttggtgga gcaaggattc
    26881 cagccatcgc aggcggcagg ggtgatgttt gcactgcaaa tgggggcggc aagcgggacg
    26941 ttaatgttgg gcgcattgat ggataaactg cgtccagtaa ccatgtcgct actgatttat
    27001 agcggcatgt tagcttcgct gctggcgctg ggaacggtgt cgtcatttaa cagtatgttg
    27061 ctggcgggat ttgtcgcggg gttgtttgcg acaggtgggc aaagcgtttt gtatgctctg
    27121 gcaccgttgt tttacagttc gcagatccgc gcaacaggtg tgggaacagc cgtggcggta
    27181 gggcgtctgg gggctatgag cggtccgtta ctggccggga aaatgctggc attaggcact
    27241 ggcacggtcg gcgtaatggc cgcttctgca ccgggtattc ttgttgctgg gttggcggtg
    27301 tttattttga tgagtcggag atcaagaatg cagccgtgtg ccgatgcctg acgcgcctta
    27361 ttaagcaggg gcgaaaggga cttgccccca atacacctac ggcgtttaca ggtatactcg
    27421 ctaaaaatta ttcagcgggt ttggaaacaa agatggcaaa acttacctta caagagcagt
    27481 tgctcaaagc gggactagtc accagcaaaa aagcggcgaa ggtcgagaga acggcgaaaa
    27541 aatcgcgtgt tcaggcgcgt gaagcacggg cggcggtaga agaaaataaa aaggcacagc
    27601 ttgagcgtga taaacagctt agcgagcaac aaaaacaagc ggcgttggcg aaagaatata
    27661 aagcccaggt gaagcagctc attgaaatga accgaattac cattgccaat ggcgatattg
    27721 gttttaactt cactgacggc aatctgatta agaagatttt tgtcgataag ctcacgcaaa
    27781 cgcagttgat taatggtcgt ctggcgattg cccgcttgtt ggtcgataac aatagcgaag
    27841 gtgaatacgc cattattccc gccagcgttg ccgataaaat tgctcagcgt gatgccagca
    27901 gtattgtgtt gcacagcgcg ctcagcgccg aagagcagga tgaagacgat ccgtatgccg
    27961 acttcaaagt gcctgatgat ttgatgtggt aaacattgat aaatcgtgtg cagatgcctg
    28021 atgcgacgct tttcgcgtct tatcaggcct acggtttacg agcgaaatgt gggccggata
    28081 aggtttacgc cgcatccggc atgcgtgcgc ggatgctcaa cgcatattca gtttattggc
    28141 gtggtaggca atatgctcgc caataaaact ggagacaaaa taatagctgt gatcataacc
    28201 ctcgtgataa cggattaacg tcttgatatt catctcctgg cagatctttt caagatttgg
    28261 agtccgcagt tgttccgcgt aaaaatcatc actcaacccc tgatcaacca tgatttccgc
    28321 aacgcgttga ccttgtgaaa taagactcac cggatcgtaa tccaaccagg catctttatt
    28381 ttcaccaaga tatgcggcaa aggcttgctg tccccacggt acttgcgatg gggagacaat
    28441 gggcgaaaac gccgagacgc tgacatattc atctgggtta cgtaacgcca gcaccagcgc
    28501 gcccagcccg cccatagaat gacctgcgat tgactttctg gccgttgccg gaaaatgatg
    28561 catcactaaa ttcggcagct cgttgcggat atagtcatac attttgtaat gttcattcca
    28621 cggcgcttgc gtcgcgttca ggtaaaaccc ggcaccttgc ccgagatcgt aacggtcagc
    28681 atctgcgaca tgactgcctc gcggactggt gtccggcgca acaacaataa tgttgtgctc
    28741 agccgcgtaa cgctgcatcc ccgatttagt aatgaaattt tgctcgttgc aggtcaggcc
    28801 tgaaagccag tacaacaccg gcaatttttc attctcggct tttgggggga gatagacgcc
    28861 gacattcatt tcacatttca gtgattggga ataatgccga tacacatttt gccagccgcc
    28921 aaagctggca tgtttttcaa tgagttccat cagtcattat ctcacggtta atcccccgga
    28981 cggctgcccg gggagagtat taagaacccc atctgaactg gacggggtaa accagcggga
    29041 aatcagtaac gaattacggt tcgaatggat ttaccttcat gcatcaggtc gaaggcgtca
    29101 ttaatttcat ccaggctcat ggtatgcgtg acaaacggtt ccagatcgat atcacctttc
    29161 atcgcatctt caaccatacc cggtaactgg gaacgacctt tcacgccgcc aaacgcggaa
    29221 cctttccata cgcgaccggt gaccaactgg aatggacggg tggagatttc ctgaccggag
    29281 cctgctaccc cgatgatcac cgactgaccc cagccgcggt gcgcgctttc cagcgctgca
    29341 cgcatcacgt tgacgttacc gatgcattca aaggtatggt cgatacccca tttgttgata
    29401 tccagcagga catctttaat cggtttgtcg tagtcattcg ggttaatgca gtcggtggca
    29461 ccgaagcgac gcgccagttc gaatttcttc gggttggtat cgatagcgat aatccgtccc
    29521 gcttttgcct gacgcgcgcc ctgaaccact gccagaccaa tcgcgccaag accaaacacg
    29581 gcaacagaat cacctggctg gactttagct gtgttgtgta ccgcgccaat accggtggtc
    29641 acgccacagc ccagcaggca gacgtgttca tggtttgctt ctggattaat tttggccaga
    29701 gacacttccg ctaccacggt gtattcactg aatgttgagc atcccatgta gtgataaagc
    29761 ggctgcccgt tgtaagaaaa acgggtggtg ccgtctggca tcaagccttt gccctgcgtt
    29821 tcgcgaaccg cgacacacag gttggttttg ccagaacgac aaaactcaca ctcgccgcac
    29881 tctgcggtgt aaagtgggat cacatggtcg ccaggtttga cgctggttac gccttcaccg
    29941 acttcaacta caaccccggc cccttcgtga ccgagaacca ctgggaagac accttccggg
    30001 tcatcgccgg agagggtaaa tgcgtcggta tggcaaacac cggtatgggt gactttaatc
    30061 agcacttcac cttttttcgg tggtgcaacg tcaatttcaa cgatttccag cggtttaccg
    30121 ggagcaaatg caacggcagc acgtgatttc atctctcgct cttcctcaat atggtaatag
    30181 attcagctat ttaagatagg cacgaaccag ttcaatagtg tcgtcaacgg attggctgac
    30241 ttcacggctg tagcagtcat ttcggtcaaa cgtttcccgg atatggcttt caagcacttc
    30301 tgccatcagt ccgttagccg cgccccgaac ggcagcgatc tgttggagta tggcacggca
    30361 ttcggcatca ccctccagcg accgttccag agcatcaatc tgcccccgaa tacgacgaac
    30421 tcgagtaagg acctttttct tctcttccgg agtactgggc atttcgcacc tcatcatctg
    30481 cacgcaatat actatagggg ggtatgctat atgtcaatgc atacccccct atagtatata
    30541 acactggaga ataaaattta tccggcccca gtatttgtag gcatgataag aggcgtgagc
    30601 gtcacatcag gcgatgtttg ttaaaagtag caaaatgcgc aaactcgcta accattgccg
    30661 atttttttct aaaaacgctt tgttattaac aagacgtttt aattccttta tcaaacttac
    30721 ccgcaacgaa atactgctcc cataacaaga cgggggagca gacaatcatg gcaatttcat
    30781 cgcgtaacac atttcttgcc gcactggcat tcatcgcttt tcaggcgcaa gcggtgaacg
    30841 tcaccgtggc gtatcaaacc tccgccgaac cggcgaaagt ggctcaggcc gacaacacct
    30901 ttgctaaaga aagcggagca accgtggact ggcgtaagtt tgacagtgga gccagcatcg
    30961 tgcgggcgct ggcttcgggc gatgtgcaaa tcggcaacct cggttccagc ccgttagcgg
    31021 ttgcaaccag ccaacaggtg ccgattgaag tcttcttgct ggcgtcaaaa ctgggtaact
    31081 ccgaagcgct ggtggtaaag aaaactatca gtaaaccgga agacttgatt ggtaaacgca
    31141 tcgccgtacc gtttatctcc accacccact acagcctgct ggcggcgctg aaacactggg
    31201 gtattaaacc tgggcaagtt gagattgtga acctgcagcc gcccgcaatt atcgccgcat
    31261 ggcagcgggg agatattgat ggtgcttatg tatgggcacc ggcggttaac gccctggaaa
    31321 aagatggcaa ggtgctgacc gattctgaac aggtcgggca gtggggcgcg ccgacactgg
    31381 acgtctgggt cgtgcggaaa gattttgccg agaaacatcc tgaggtcgtg aaagcgttcg
    31441 ctaaaagcgc catcgatgct cagcaaccgt acattgctaa cccagacgcg tggctgaaac
    31501 agccggaaaa catcagcaaa ctggcgcggt taagcggcgt gccggaaggt gacgttccgg
    31561 ggctggtgaa ggggaatacc tatctgacgc cgcagcaaca aacggcagaa ctgaccggac
    31621 cggtgaacaa agcgatcatc gacaccgcgc agtttttgaa agagcagggc aaagtcccgg
    31681 ctgtagcgaa tgattacagc cagtacgtta cctcgcgctt cgtgcaataa aaggaggcgc
    31741 agatgctgca aatttctcat ctttacgccg attatggcgg caaaccggca ctggaagata
    31801 tcaacctgac gctggaaagc ggcgagcttc tggtagttct ggggccgtct ggctgcggca
    31861 aaactacgtt gctgaatttg attgctggtt ttgtgcctta tcagcatggc agcattcaac
    31921 tggcgggtaa gggtattcag ggaccgggag cagagcgtgg cgtagttttt cagaatgaag
    31981 ggttgttacc gtggcgcaat gtacaggaca acgtggcgtt cggcctgcaa ctggcaggtg
    32041 tagagaaaat gcagcgactg gaaatcgcac accagatggt gaaaaaagtc gggctggaag
    32101 gcgcagaaaa acgctacatc tggcagcttt ccggtggtca acgtcagcgg gtggggattg
    32161 cccgcgcgtt ggcggcaaat ccccagctgt tattactcga cgaaccgttt ggtgcgctgg
    32221 acgccttcac ccgcgaccag atgcaaaccc tgctgctgaa actttggcag gagacgggaa
    32281 agcaggtgct gttgattacc cacgatatag aagaagcggt gtttatggcg actgaactgg
    32341 ttttgctttc acccggccca ggccgtgtgc tggagcggct gccgctcaac tttgctcgcc
    32401 gctttgttgc gggagagtcg agccgcagca tcaagtccga tccacaattc atcgccatgc
    32461 gcgaatatgt tttaagccgc gtatttgagc aacgggaggc gttctcatga gtgtgctcat
    32521 taatgaaata ctgcattcgc atcggctgaa atggcgctgg ccgctctcgc gtcaggtgac
    32581 cttaagcatt ggtacgttag cggttttgct cactgtatgg tgggcagtcg cggcgctgca
    32641 actgattagc ccgctgtttt tgccgccacc gcaacaggta ctggcaaaac tgctcaccat
    32701 tgccgggccg cagggattta tggacgccac gctgtggcag catctggcag ccagcctgac
    32761 gcgcattttg ctggcgctac tcgcggcggt gttgatcggt attccggtcg ggatcgccat
    32821 ggggctaagc ccgacggtgc gcggcattct ggatccgata atcgagcttt atcgtccggt
    32881 gccgccgctg gcttatttgc cgctgatggt gatctggttt ggtattggcg aaacctcgaa
    32941 gatcttgctg atctatttag cgatttttgc gccggtggcg atgtcggcgc tggcgggggt
    33001 gaaaagcgcg cagcaggttc gcattcgtgc cgcccagtcg ctgggtgcca gccgtgcgca
    33061 ggtgctgtgg tttgtcattt tgcccggcgc gctgccggaa atcctcaccg gattacgcat
    33121 tggtctgggt gtgggctggt ctacgctggt ggcggcggag ctgattgccg caacgcgcgg
    33181 tttaggattt atggttcagt cagcgggtga atttctcgcg actgacgtgg tgctggcggg
    33241 gatcgcggtg attgcgatta tcgcctttct tttagaactg ggtctgcgcg cgttacagcg
    33301 ccgcctgacg ccctggcatg gagaagtaca atgagtgaac gtctgagcat taccccgctg
    33361 gggccgtata ttggcgcaca aattacgggg gccgacttaa cgcgcccgtt aagcgataat
    33421 cagtttgaac agctttacca tgcggtgctg cgccatcagg tggtgtttct gcgcgatcaa
    33481 gctatcacgc cgcagcagca acgcgcgctg gcccagcgtt ttggcgagct gcatattcac
    33541 cctgtttacc ctcatgctga aggggttgac gagatcatcg tcctggatac ccataatgat
    33601 aatccgccgg ataacgacaa ctggcacacc gatgtgacat ttattcaaac gccacccgca
    33661 ggggcgattc tggcggcgaa agagttaccc tcgaccggcg gcgatacgct ctgggccagt
    33721 ggtattgccg catatgaggc gctctctgtt cccttccgcc agctgttgag cgggttgcgt
    33781 gcggagcatg atttccgtaa atcgttcccg gaatacaagt accgcaaaac cgaggctgag
    33841 catcaacgct ggcgtgaggc ggtcgcgaaa aatccaccgt tgctacatcc ggtggtgcga
    33901 acgcatccgg tgaccggtaa acaggcactg tttgtgaacg aaggttttac cacacgaatt
    33961 gttgatgtca gcgagaaaga gagcgaagcc ttgttaggtt ttttgtttgc ccatatcact
    34021 aaaccggagt ttcaggtgcg ctggcgctgg caaccgaatg atattgcgat ttgggataac
    34081 cgagtgactc agcactatgc caatgcggat tacctgccac agcggcggat tatgcatcgg
    34141 gcgacgattc ttggggatat tccgttttat cgggcggggt aagggggaaa tgccgggttg
    34201 gataacccgg cataatgatc attaacgcag aatcttcttc tcagccaaat ccagcgcaaa
    34261 gtagctgaaa atcagatccg cacccgcacg cttaatcgaa cctaagcttt cgagcacgac
    34321 tttctcttca tctatagcac ccgccagcgc ggcgaactta atcatcgcgt actcaccgct
    34381 cacctgatac gcgccaatcg gcaattcagt acgttcacgc agctcacgca cgatgtcgag
    34441 gtatgctccg gcaggtttaa ccatcaggca gtctgcgccc tgggcttcat ccagcagtga
    34501 ctcacgaatc gcctcacgac ggttcattgg gttcatctga tagcttttgc ggtcgccttt
    34561 taatgcgctt ccggcagctt cacggaacgg gccataaaag gaggaggcga acttggtcga
    34621 atacgacata atcgccgtat ctttaaagcc cgcagcgtcc agcgcctggc gaatcgcctg
    34681 tacctgacca tccatcgcgg cagaaggggc gatgaagtct gcgcctgcag cagctgcaac
    34741 cacggcttgc ttgcctaaat tttccagagt cgcgtcgttg tcgacgccat gctcgcacag
    34801 aacaccgcag tgaccgtgag atgtgtattc acagaagcag gtgtctgaca taacgatcat
    34861 ttctggcacg gtctgcttgc agatgcgcga catacgcgcc accagtccat cttcccgcca
    34921 ggcatcgctg ccggtttcat cggtatggtg agagatgccg aaagtcatca cggaacgaat
    34981 accggcgttg gcgatgcgtt caatttcgcg tgccagatgt ttctctggaa tgcgcatcac
    35041 gcctggcatg gcttcaacgg ctttatagtc gtcaatttct tcttcaacaa agatcggcaa
    35101 caccaggtcg ttaaggctaa gtgttgtctc ttcaaacata gcgcgcagcg caggagattt
    35161 gcgcaggcga cgagggcgtt ggattaagtc tgtcatggtc tgcctgatgt ttgtggaatc
    35221 gaggggcata gtatacctga agcaggctgg gtgtgtttta cgaaagttgt cgcgatgttg
    35281 acaagaa
//
LOCUS       DACADF010000033        35089 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.033, whole genome shotgun sequence.
ACCESSION   DACADF010000033 DACADF010000000
VERSION     DACADF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.033"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..292)
                     /locus_tag="ISB30_001560"
     CDS             complement(<1..292)
                     /locus_tag="ISB30_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAP1989205.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAEKNLEKNLNPSLLLILAD"
     gene            complement(550..1803)
                     /locus_tag="ISB30_001561"
     CDS             complement(550..1803)
                     /locus_tag="ISB30_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAP1989206.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2035..3366
                     /gene="argA"
                     /locus_tag="ISB30_001562"
     CDS             2035..3366
                     /gene="argA"
                     /locus_tag="ISB30_001562"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAP1989207.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQTRVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3448..5274)
                     /gene="recD"
                     /locus_tag="ISB30_001563"
     CDS             complement(3448..5274)
                     /gene="recD"
                     /locus_tag="ISB30_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAP1989208.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTAEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     SHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEVLAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(5274..8816)
                     /gene="recB"
                     /locus_tag="ISB30_001564"
     CDS             complement(5274..8816)
                     /gene="recB"
                     /locus_tag="ISB30_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAP1989209.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKRLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGEMQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDVAGLRTC
                     IEALCDDDIAWQTAQIGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEVPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITAVLLAPLNETGISLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(8809..11697)
                     /gene="ptrA"
                     /locus_tag="ISB30_001565"
     CDS             complement(8809..11697)
                     /gene="ptrA"
                     /locus_tag="ISB30_001565"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAP1989210.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQAITLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKIADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11873..15241)
                     /gene="recC"
                     /locus_tag="ISB30_001566"
     CDS             complement(11873..15241)
                     /gene="recC"
                     /locus_tag="ISB30_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAP1989211.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15254..15577)
                     /locus_tag="ISB30_001567"
     CDS             complement(15254..15577)
                     /locus_tag="ISB30_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP1989212.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15562..15969)
                     /locus_tag="ISB30_001568"
     CDS             complement(15562..15969)
                     /locus_tag="ISB30_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAP1989213.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15966..16529)
                     /locus_tag="ISB30_001569"
     CDS             complement(15966..16529)
                     /locus_tag="ISB30_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAP1989214.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(16520..16990)
                     /locus_tag="ISB30_001570"
     CDS             complement(16520..16990)
                     /locus_tag="ISB30_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAP1989215.1"
                     /translation="MKKQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17175..17969)
                     /gene="thyA"
                     /locus_tag="ISB30_001571"
     CDS             complement(17175..17969)
                     /gene="thyA"
                     /locus_tag="ISB30_001571"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP1989216.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17976..18851)
                     /gene="lgt"
                     /locus_tag="ISB30_001572"
     CDS             complement(17976..18851)
                     /gene="lgt"
                     /locus_tag="ISB30_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP1989217.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19002..21248)
                     /gene="ptsP"
                     /locus_tag="ISB30_001573"
     CDS             complement(19002..21248)
                     /gene="ptsP"
                     /locus_tag="ISB30_001573"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP1989218.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21261..21791)
                     /gene="rppH"
                     /locus_tag="ISB30_001574"
     CDS             complement(21261..21791)
                     /gene="rppH"
                     /locus_tag="ISB30_001574"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAP1989219.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            22476..23165
                     /gene="mutH"
                     /locus_tag="ISB30_001575"
     CDS             22476..23165
                     /gene="mutH"
                     /locus_tag="ISB30_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAP1989220.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23234..23947
                     /locus_tag="ISB30_001576"
     CDS             23234..23947
                     /locus_tag="ISB30_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAP1989221.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24085..24303
                     /gene="ygdR"
                     /locus_tag="ISB30_001577"
     CDS             24085..24303
                     /gene="ygdR"
                     /locus_tag="ISB30_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAP1989222.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24411..25451
                     /locus_tag="ISB30_001578"
     CDS             24411..25451
                     /locus_tag="ISB30_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAP1989223.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHSLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25483..26676)
                     /gene="lplT"
                     /locus_tag="ISB30_001579"
     CDS             complement(25483..26676)
                     /gene="lplT"
                     /locus_tag="ISB30_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAP1989224.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLIALAACALAYGGAVIANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26669..28828)
                     /locus_tag="ISB30_001580"
     CDS             complement(26669..28828)
                     /locus_tag="ISB30_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-acylglycerophosphoethanolamine
                     acyltransferase/acyl-ACP synthetase"
                     /protein_id="HAP1989225.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQLKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENIRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29414..30445
                     /gene="galR"
                     /locus_tag="ISB30_001581"
     CDS             29414..30445
                     /gene="galR"
                     /locus_tag="ISB30_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAP1989226.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30452..31714)
                     /gene="lysA"
                     /locus_tag="ISB30_001582"
     CDS             complement(30452..31714)
                     /gene="lysA"
                     /locus_tag="ISB30_001582"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP1989227.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31836..32771
                     /gene="lysR"
                     /locus_tag="ISB30_001583"
     CDS             31836..32771
                     /gene="lysR"
                     /locus_tag="ISB30_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAP1989228.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32758..33450)
                     /gene="ygeA"
                     /locus_tag="ISB30_001584"
     CDS             complement(32758..33450)
                     /gene="ygeA"
                     /locus_tag="ISB30_001584"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAP1989229.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADDRAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33579..34997)
                     /locus_tag="ISB30_001585"
     CDS             complement(33579..34997)
                     /locus_tag="ISB30_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP1989230.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8659 a         9599 c         8628 g         8203 t
ORIGIN      
        1 gatccgcaag aatcaatagt agcgatggat tgagattttt ttcgagattt ttctccgcat
       61 attgcactat tttttcggca acaaacacat actcaatatc tacatgttca atcaatttat
      121 agagtttgtt tttttgttca tttctgacat aaaaaatccg gttagccgga tgctcaggga
      181 ccgacattcc atattttttg ttgtaaccga cgccaggccc agaaatgata acctcctgtc
      241 cattcatcga tgcctgtaca caattattgt tcatgacttt ttcgatgatc attccatggc
      301 tccaaaaaaa aaggcaaggc acgtcagaag taataactac ttcaaacatt gccttgcctg
      361 atttaacagt tacaagatga ttaactaatg cgtatacatt acacggaatt gtgcttaatt
      421 aaagagatgg tcggcttatt tttcaagccg acattcaaat atccgacatc agtcacaaaa
      481 ccagcgtcta atattcaggt aaatgttatc tattcgacga aatataaatt cattgaataa
      541 ataaaaacat tatccccttc tcgccagcgt cgccccatcg gcaaaatacg ctttaattcc
      601 cgcaagaata gactccgcaa cttcctgctg gaaagtcgcc gttttcagtt tacgctcttc
      661 ctcaacgtta ctgataaacg ccgtttcgac cagaatggag ggaatatctg gtgcctttag
      721 tacggcaaac ccagcctgtt caacttgatt tttatgcagc ttgttgattt taccgagctt
      781 attcagcacc gctttaccaa acttaaggct gtcggcaatg gtcagcgact gcaccatatc
      841 gaacatggtg tggtcgacat agcggtcacc gcttttgctt acgccaccaa tcaagtccga
      901 ggcgttctgg gtttgtgcca gatattttgc cgcagtactg gttgcgcctt tggttgagag
      961 cgcaaacacc gaggaaccgc tcggctggcg actggtaaag gcgtcggcat ggatagaaac
     1021 aaacaagtca gcgcgctgtt tctgggcttt tgctacgcgc acttgcaacg gaatgaagat
     1081 gtcttcattg cgcgtcatgt acaccttcat attgccctct ttctcgatca gagagcgcag
     1141 acggcgagct atttgcaata ccacgtcttt ttcgcgtgtt ttgtatttcc ccaccgcacc
     1201 ggagtcttcg ccaccgtggc cagggtcaag cataatgaca atcggacgat cccgccctgc
     1261 tttacccggt tgtggaccac tttgtgctgg cggcacctgc ttttcgaggt cgcctttgtt
     1321 gtaatcctcc agcagcgcca gcagcgggtc ctgcatatcc tgtgcattgg ccggatagag
     1381 gtccatcacc agacgctctt taaacccggc gaccggcgca agggcaaaca gctgcggttt
     1441 tacgttttgc tttaattcaa aaaccatacg tacggtttgc gggtcaaatt gcccgacgcg
     1501 cgccgacttg atgaacgggt cgtcagcgcg gatttgcgca gccatcccct tgagcactga
     1561 gttcaggttt acatcttcga tatccaccac cacgcgttca ggattactca acgcgaactg
     1621 cttatatttc agctgacgat ttgattctac cgtcacgcgg gtgtagctgg acgcaggcca
     1681 gacgcgcacc gccacgacct ggctgaccgc agccaggctg acctgactta cgctcaatag
     1741 ccacatggca cccgcgcctt gcagtaaacg acgacggctg attgcagtgt tggatcctga
     1801 catgcctctc ccgagcaaaa gaaatctaag ctgtgtaaca agtaaacgac caatttgacc
     1861 ggaaacttta gcgaaagacg cataatctgt catctataaa acggtaaaca ttctttttat
     1921 attcacggta ttactgataa aaaactcgct cccgcataaa atttacactt gcaccctgcg
     1981 aaaaaacaga ataaaaatac aataatttag aataatcatg caaagaggtg taccgtggta
     2041 aaggaacgta aaaccgagct ggtcgaggga ttccgccatt cggttcccta tatcaatacc
     2101 caccggggaa aaacgtttgt catcatgctc ggcggtgaag ccattgagca tgagaatttc
     2161 tccagtatcg ttaatgatat cgggttgttg cacagcctcg gcatccgtct ggtggtggtc
     2221 tatggcgcac gtccgcagat cgacgcaaat ctggctgcac atcaccacga accgctgtat
     2281 cacaagaata tacgtgtgac cgacgccaaa acactggaac tggtgaagca ggctgcggga
     2341 acattgcaac tggatattac tgctcgcctg tcgatgagtc tcaataacac accgctgcag
     2401 ggcgcgcata tcaacgtcgt cagtggtaat tttattattg cccagccgct gggcgtcgat
     2461 gacggcgtgg attactgtca tagcgggcgt atccggcgga ttgatgaaga cgcgatccat
     2521 cgtcaactgg acagcggtgc aatagtgcta atggggccgg tcgctgtttc ggtcactggc
     2581 gagagcttta atctgacctc ggaagagatt gccactcaac tggccatcaa actgaaagct
     2641 gaaaaaatga ttggtttttg ctcttcccag ggcgtcacta atgacgacgg tgatattgtc
     2701 tccgaacttt tccctaacga agcgcaaacg cgggtagaag cccaggaaga gaaaggcgat
     2761 tacaactccg gtacggtgcg ctttttgcgt ggcgcagtga aagcctgccg cagcggcgtg
     2821 cgtcgctgtc atttaatcag ttatcaggaa gatggcgcgc tattgcaaga gttgttctca
     2881 cgcgacggta tcggtacgca gattgtgatg gaaagcgccg agcagattcg tcgcgcaaca
     2941 atcaacgata ttggcggtat tctggagttg attcgcccgc tggagcaaca aggtattctg
     3001 gtacgccgtt ctcgcgagca gctggagatg gaaatcgaca aattcaccat tattcagcgc
     3061 gataacacga ctattgcctg cgccgcgctc tatccgttcc cggaagagaa gattggggaa
     3121 atggcctgtg tggcagttca cccggattac cgcagttcat cacggggcga ggttctgctg
     3181 gaacgcattg ccgctcaggc gaagcagagc ggcttaagca aattgtttgt gctgaccacg
     3241 cgcagtattc actggttcca ggaacgtgga tttaccccag tggatattga tttactaccc
     3301 gagagcaaaa agcagttgta caactaccag cgtaaatcca aagtgttgat ggcggatttg
     3361 ggttaagcga tgcaaatcgc cggatgcgac atgcacaaca ttcataggta tgataagacc
     3421 cacatgcgtc gcatcaggca attacgttta tccccgtgaa ctaaacaacg ccgccagacc
     3481 actgcgccgc tcggtgcgcg tggcaattgc cgcacttaaa atgcgctcat cggcatacag
     3541 cgacagacgg cgacgcgcgc gggtcaccgc ggtgtaaacc agctctcgcg ttactaccgg
     3601 cgtgcgttga ctcggtaaaa tcaacgccgc atggtcgaac tccgatccct gcgatttatg
     3661 taccgtcatc gcccacgtcg tttcgtgctc tggcaggcga ctcggttgca cagacttaat
     3721 attgccgtcc ggcatcgcaa accagacgcg cgtcccctgc ccgcgatcca gcgcaatacc
     3781 aatatcgcca ttaaacaacc caagcgcgct gtcattacgg gcaatcatca ctggtcgacc
     3841 ttcgtaccaa cgagagtgcg gatgacgatg aattttgcgc ttctgttgca taaattgctc
     3901 aattcgctca ttcaatccag ccacgccaaa cggtccttcc cgcagggcgc acaaaagctg
     3961 gtactcattg aacgcctgaa tgattaaatc cggctcggca cgcgcttgca gcagatccag
     4021 ataacgtccg taacccgcaa gaacttcctc aagcatcgca atataatctt cgccactctg
     4081 taaaagccgt ttttcgatat cagtaaaatc ctgctgaaaa acggttttca ctgccgtttt
     4141 atcacctcgg ttaatcgccg cagctaactg accaatgcca gaatcgctgc cgaaacgata
     4201 gcttttttgc agcaggcaga ggctgtcgcg caaagatgcc gcttctgtgc cagttcctgc
     4261 tggaacgtgg cttcccgtca ggcggctcaa ctgcccggca cgctcggcgg taaagcccgc
     4321 gttggcataa gcgcagatat cgcccagcac agccccagcc tcaaccgagg ccagttgatc
     4381 acgatcgccg agaaagatca ctcgcgcatg atcgggcaag gcgtcgatca gtctcgacat
     4441 cataggaaga tcgatcattg acgcttcatc taccaccagc acatcaagat gcagcgggtt
     4501 accggcatga tgacgtaaac gctggctacc cggctgcgcg cccagcaatc ggtgcaaagt
     4561 gctggcatct tccggaatgc gtttcttttg ttcagcggtc agcggtaact gtcgcaaagc
     4621 cttgccgaga gattcggtta agcgcgcggc agctttaccc gttggtgcag ccagacggat
     4681 acggcagcgt tctccgtcgg ccatttgaat taaggctgcc agcaacttcg ctacggtggt
     4741 cgttttaccg gtgccaggac cgccggaaat caccgaaatc cgccgcgtca gcgccactgc
     4801 cgccgcaact ttttgccagt taatttcatc gcttactgga aaaagtttgt ccagggtttg
     4861 cgccagtaga gcttcatcaa cctcaatggc atgattcact tcgttgaaaa aacgtgccac
     4921 tgtgcgctcg ttacaccaca tgcgattcaa ataaagacga tcgccacaga ggatcatcgg
     4981 cgttggttca tctccccggc tgaccgcttg tgaagccagc aagcattctt cccaattttg
     5041 tagctcaccg atttcactga cacaggtcgc caacagcgga tgcgacgcct cgttattttc
     5101 cagtcgtgaa agcggcaaac aaacgtgtcc ctctccggca tcatgactta atagtgccgc
     5161 cgcgagggtg acggcaggat gttcatctcc cgccacggtc agggcaaact gcacgtccag
     5221 cgggcgtagc tgtttgtgct ccacagcttc cagtaattgc ttttgcaatt tcattacgcc
     5281 tcctccaggg tcataccggc aaacatctca tccatcaggt caatcaaccc ggcgttgggt
     5341 cgggtcgcgt agatcccttg ttgcggatgt tctttatcaa cgccacgcag gaacagataa
     5401 ataacgccgc caaagtggcg ctcatagtcg taatcagcaa tacgatggcg cagataacgg
     5461 tgcagcgcca gggtataaag ctgatattgc agatcatagc ggtgtgcctg cattgccgct
     5521 gccatagcct gttgggtgta agccgaactg tcttcaccca accagttgga tttatagtcg
     5581 agcaggtaat aacgcccttc gtggcggaac accagatcga taaagccttt taacatgcca
     5641 cgtacctgca tgaactccag cggcgggcag ccagcagata gcgggtcaaa ctggcgaatt
     5701 agtgcatcaa gctggctggc gatgagcggt tcgctaattg gcaggtaaaa ctccatctcc
     5761 acctgtttat cgcgatcgga aagctgactc aggctaatac ccgtttcatt gagaggtgcc
     5821 aggaggacag ccgtgatcca ctcagtcaac accggttccc actgcggttc aaagccaccg
     5881 agctccagtt tttcctgtac ccagttcggg tcaaccggct gggtaaaatc gaggtcttca
     5941 aacaaactgt gcaagaatgt ccccggtgac gcaccgcgcg ggaactgatg cggtgttaac
     6001 gtcggtactt caacgacgct gaccacgcct gcggcatcaa catccaaccg tggcatcaga
     6061 tcctgagcga taccgtgacc acgctgctgc aaaccggagt agctggtgac acgccagtta
     6121 tcgccgggca atcgttgtag cgtcctcgca ttcagttctg cagttaatgc atcattaacc
     6181 tgccagggct ggttatcacc aatttgtgcc gtttgccagg caatatcatc atcgcacaac
     6241 gcttcaatgc aggtacgaag ccctgccaca tcttgcggtt ctcctttttg cagcaaacgc
     6301 ccgagcgcac tttggtggac gtcggtatca ccttttttat cgccacgacg gcgcaccagc
     6361 ggtgcaacgc cgagactgca atgccaaacc gaacgcgtca gcgccacgta aagtaaacgc
     6421 agatcttccg ccagacgttc ggcctccgcg aggtcgacgc tttctggcgc agcattaaga
     6481 tccagaacgg cctcaaacga gtggcgatcg tgataaaacg cctgatcctg gacgcggaaa
     6541 ttggtaataa atggcaacca gaccagcgga tattccagcc cttttgattt gtgtatcgtg
     6601 acaatctgca ccagatgttt atcactttcg aggcgcattt gttggctgga ggcattactg
     6661 tctggctcga ggatatgttg cgataaccag cgtaccagcg catgttcact ttccagctgc
     6721 gttccggctt cttgtagcag ttcgctgata tgcaagatat cggtaagacg ccgctcaccg
     6781 cctgccgttg ccagcaagtt ttctgcaatg ttacgcgccg acatcagcgc ccgcagcatc
     6841 ggcataacgc cacgtttgcg ccagatttgc cgataaccat cgaactcttc gactaccgca
     6901 tcccacgcat gttcgtcatt attcagcgtt tcaatatcca gcgcattcag ccccatcatt
     6961 gacgttgcca gcgcactgcg cagggtgttc tcacgttcgg gcgtcatcac cgcctgcaac
     7021 agccaaagca tctcctgtgc ttccagagtt tcaaaaacac tgtcgcggtt cgaaaggtaa
     7081 acggaaggga tttccagcag cgttaaggca tcgcgcacct gagcggcctc ctggcggctg
     7141 cgcaccagca cactgatgtc cgaagcacgc accggacgcg catcgtcgcc gttcatcagc
     7201 aacgcttcgc cccgctgtcc ggcttgtagc cagtcgcgga tttgcgcagc acatacctgc
     7261 gccatggtac tttgatagtc gccaacgccg cagctttcgc cttccatcag ccacattttc
     7321 atcgcaggct gcatttcacc tttaaacaca aagcgtaacg cctgattttt cccggcagat
     7381 ttcacaggaa taaacggtat ttcacgaaac atgaacgcgt catcagtctg gctgaaaagt
     7441 ttgttaacgc tgttcaccat tcccggtgcg gaacgccagt tggtatctaa ggtgtagtga
     7501 gcatgaactt cgctacgcgc cttcatataa gtgaagatat ccgcaccccg gaatgcatat
     7561 atggcctgtt tcgggtcgcc aatcagcaac aatgcggttt ccggctgatg gtgccagata
     7621 cggcgaaaaa ttcggtactg ctgggggtcg gtatcctgaa attcatcgat cattgccacc
     7681 gggaatcgcg tacggatcgc cgctgccaat acctcgccgc tttcactacg cagcgcggaa
     7741 tcgagccgac ttaacatgtc atcaaaaccc aattcgccac ggcggcgttt ttcacgcgct
     7801 actgtttcgc ggatctcagc caatgcgcgg gtgatcacca gatcgcggat cgacaatggt
     7861 tctgcaagca gttgatcgat cgcctcaaac agtggatgtc gcggggtttc ccccccggct
     7921 ttcgtgcgat cttctaagaa acgctgagag aatttttcca gcgactccgg caactgatag
     7981 ctattggtct cttcttctgc ccaggcgctg atcttctcga tccatttagc ctgattgcta
     8041 cggttaaact tgcgtcgatc aataccagaa gattcgatca gcgcatccag ttcacccact
     8101 gcgtcgcgcc actgctgttt tacggcatca atacgcgcca caatttgcgc gtggcgggaa
     8161 gccagcgttt catcatcggg gggcggtgct ttgataactg gcgcttcgcc ttgcagataa
     8221 cgattaatat cgcgcagcaa cgcctgcggc cctttccagg tttcaaagac gacctgggca
     8281 atttcacgcg gcagcgggta gcagtggcga cgccagaaat cggcgcaggc ctggtagcgt
     8341 agcagagact catcttcaat cagctgctgc tcaaacagca tgccggattc aaaggcattc
     8401 aggttgagca tgcgctggca aaagccgtgg atagtaaaga ctgccgcttc atccatctgc
     8461 cgctcggcta acaacaacca ctgcgcggct tgcgctttat cgtcgatctc ttccagcagg
     8521 cgtttgtaca gtggattgtc ggtggtttca cgcagacagg cgatgcgcaa ctcgtggata
     8581 ttgctacgga tacgaccacg caattctgcc gtggcagcct cggtaaaggt caccaccagc
     8641 agttcttcaa cggtcagcgg gcggggaaag gcggccgaac cgcctagtcc aagtaacagg
     8701 cgcaaataga gcgccgcaat cgtaaaggtt ttgcctgtgc cggcagaggc ttcaatcagg
     8761 cgctcgccct gtaagggcaa gcgcaaagga tctagtgtct cggcgacatc actcattctt
     8821 ttcactcatc aggggcattg tttgctgcaa cgcgctgacg ttctcccaca ctttccagcc
     8881 ttcaggatgc acatattcgg ctttcccatt ctggctgccg gaaatctgcg acagaatagc
     8941 cataccttgc ggctcgacca ccgcctgatg gaagaaatca gcaatttttt gcggcgtcag
     9001 cagttttatc tgggccacga ttttatcacg cgaatcgaag cgcatattgc cgcgatcgaa
     9061 atctttactt aacttcgatg cttcttcgcc gagcgtttgc ggtgcctgca gcatctgggt
     9121 aattaccgcc tgctggattt gcgcaaactc ttctggcttc atcgcccgca attttgcctc
     9181 tgcggttggg aaaaacgcct tgtaacgctc ccacaagaat gaaggctgtt tatcattgct
     9241 ttgcaaaagg aagcccatgc cccactgacg ccccacgctc attggaaacg caaacacggc
     9301 atagcccaac tgttcttcgg tacgcaactg attgtagaac cacggctgta cgatctgccc
     9361 caacagagaa ctatacgctg agctggtgta ttcatcgtag ccagtcggta caaataccgc
     9421 tgccagtgcg gagtcggtgc tgttaccggc tttttcaaag atgacggatt gttttttatc
     9481 gaccacgaca tctttgttac gacaccactc tgaaccatca gcgcccaact gtttttgcac
     9541 atggcgtgcc agcgttattg cctgggcttc agtcatgttg ccgataacca taaactctgg
     9601 tctagcccct gattttaagg cgtcgcgata ggccagcacc tctttcaacg taatggaggg
     9661 caaaattttg cgccgttcat ctcgcgagaa gtacggcact tgcgagagca tctgcgcggg
     9721 cataatcgcc tgctcgaacg ctttgccctt ttctgcggaa tccatcatct ggttatacca
     9781 ggacttcgcc tgctcaagct gatcttccgt agcggtatag ctaaagtagc cctccagcag
     9841 agcctggaac agctgcggca gacgctgggt gtaaccatta gcattaacca taaggccgtt
     9901 gttagcattg gtggaaaaac ttatgccacc aaccgacgcc tggttgctta actgatcaag
     9961 cgccagccct gcgagataat cattgagtgc aaacatcacc tgattgcggg cgctgtccat
    10021 ggctttcgga ttacgcaaaa tcaggctgac atcagctttg ggctcgctgg caaaataacg
    10081 gcttggcgca tacaccacgc gcagattcga ctcatcaaca atcaactctg gatggtcgta
    10141 tttcttctct gacttaatca gcgagaaatc gtcaggaata taggggttaa gctccggtaa
    10201 ggagagcgca atattggcgg cttttttctg ccagtcagcg aaagtttgtg cgctaatttt
    10261 atcgacctga tacggcgcat cgacaaagta agccgttttg ttgtgcggct ctttcgggct
    10321 gatataccag atacgcgcat tctgtggcgt catcatcgcc agacgttctt ttaccgcttt
    10381 agcgtcgtac cgatcggcaa tattgactgc atccagtgta tgctcaacag gaacgcgaat
    10441 catggtatct gccagccatt cgacgtaatc catatcacgg gtgattgacg gataacggaa
    10501 gtcgatatcc agcacattcg ccagttcatc gaagtattgt ttatcgatcc ctttttcacg
    10561 taacagattg aggtagctaa aaatggccgc cacaacctga tcgcgattcg ccagaccttt
    10621 atcggttaaa gacgcagaga tcgctaatac gccgctgttg ccgttgacga taggatcgga
    10681 attggcgcta atgccctcaa ctaatccctg cttttgcagc cagtcagaaa gtgtaccagg
    10741 gctgcgatta ccaatcagat aggtaatcaa ttcatccgtt ttactacgga acttcgctga
    10801 attgttatcg atgcgaaact caacgcgcag aactttacgc ggcagcgccg ggacgtaatg
    10861 aatgataatg cccttttgcg cgtcggtgac taccggcacg gtgatttccg gttttttgct
    10921 ctctttgttc ggcacgcgac caaaggtgtc cgccgccatt ttcgccaact ccggcaacgg
    10981 tttattgctg taaataaccg ccttcatcag attggcggaa tagtacttct cgtggaaatc
    11041 tttcagcgcc tgctgtaccg gattacctgg tttgtcgctt aaagtttcga ggttaccacc
    11101 agaaaacttt gaaccggggt gtgccgggtt aatggtttct gcgctgacct gtgccatgcg
    11161 catcccgtca cgcgtacgcg ccatggttaa ttcggcattc actgcattac gctcacgttc
    11221 ggcgtatttc ttgtcgagca agggttctgc aatagcatcg gccaggcggt ctaccgcacc
    11281 gggtaaggca tcgttctcaa cttccagata gaaagccgtg cgatacggtg ccgtgctggc
    11341 attgtgactg ccgccgtgca ttttgagata ttcggccaga ctgtcagcct gcggatactt
    11401 tttcgacccc atcagactca tatgttcaag gtaatgtgcc agcccctggt acgcctcagg
    11461 atcttccagc gacccaacgg gcaccaccag cgccgagagc gatttaactg cctgcggatc
    11521 agaaaccagt aagaccacca taccgttatc cagacgtata gcctgatact ggcggttatc
    11581 tttatcactt ttacggatgg tttcctgaat cggctgccat cccgtttctg cctgacttaa
    11641 gggtgcccag agggcaacaa acaacaataa tgctttgaac cagatgctgc ggggcattca
    11701 cggacctcat aatcaacttt attttctggc cagattcaac acgttaacgc aaatcatctg
    11761 ccagaattta atcttgtgct gaacgagtca gcctatgtct atataaccat cagtccgtga
    11821 ctggtgcgca tcataaagta agcggataga ttgcgcaatt tttatacaac actcatgact
    11881 gattaaagcg aaacagcggt aacaggaaac gttgcgactg ttcaacgata gcctccattg
    11941 tctctggtgt taattgccgc cagagccgtt gataccagat atcatcacct tcgccacgca
    12001 ccatcatgtt gccttcgtaa gcctgaagga atttcgtacg ggctttttgc aacgtggaat
    12061 cgtcatccag catggcatcg ttttgcgcgt cataacaggt ttttagccac gcgccgccac
    12121 tttcaggtaa caccagcaat ggcgcggaca ttccttcacg atacccctca atcagttgtg
    12181 agaggtaatg caaagcctgt tcggctgcaa gcggcggaaa acgccactcg ccgtctttgc
    12241 gtagaaaaag gcgactttca ccattaccac cgctggcaca gtagacaagg tgttccagcc
    12301 aaagttgcat tccctgcgcc acacttaata aagagggacg ccagcgcaac aggccatccg
    12361 gctgcacctg cggcaaccag cccgttattt gcacaccgtt gcaggcgaga tcaatctcca
    12421 tactctgccc cggctggcga caggcaatga ctctgtcggc aagctgctgc atctcctgac
    12481 actgcgtttc ccagaaaatt tcaccaaaag cgccatacgg taaatcccct gccgcccgga
    12541 agcgacggaa caagcgttcg gcatcatcct gctcaaccag tgcattcaat aactgctgat
    12601 tgatttgata acggctaagt ccttccagaa taaatggctc ggtgtcgggg atttcgctgt
    12661 cttcggtacg gaagttcacc tgcaaacgca tctggaaaaa tgcccgcacc ggatgtgccc
    12721 agaatcgttg tagcgtttcc agcggcacgg tttccggtaa ggtaaatggc agcggctgaa
    12781 caaattcaga atgtgcttta ccagcctggc tggccgcagg tagccattca cgagcataac
    12841 tttgtcgttc gcctggctgg tagttttgtg gatcaaacgg cattcgggta tggaggcaag
    12901 taagatgcgc ttttaccctt gcctcgcttt catcacagtt gagcgcttca tcgcccggta
    12961 gataatggct ttgcccgatg tagtcgatca gttcctgcac cagtaccgac gggaaacgtt
    13021 cactgttatc ctgaatggaa cgcccgatat agctgatata gagtttttgc tgcgcggaaa
    13081 ttaacgcttc caggaacaga tagcggtcgt catcgcgacg gctacggtcg ccacgcttcg
    13141 gtttctggct catcagatca aagcccaatg gcgcaagctg acgtggataa acgccgtcgt
    13201 tcattcccag caggcaaacc actttgaacg gaattgaacg cattggcatc agagtacaaa
    13261 tgttaaccgg tccggcgaga aaacgctggc tgatacgttc ttgatccagg cgctgtgcca
    13321 gttcatcacg caatagtgac agcggcaccg cgtcgccata ctgcgcacct aaaccttcgg
    13381 cgataatcgc ctgccattgt tgttcgatca gcgtcatcgc cgcttcggtt tccgcatccg
    13441 gcaggaagaa ggcgttgagc atatcgcgac aaaccggcaa ccactcttcc agcggacgct
    13501 cctgcgccag cccgcgacgc cagatattta gctgcattag cagtgaagcc agatgcccca
    13561 ccagttctgc aattaagccg ctcgattcat cataaggtag aaccgattgc cactcgccct
    13621 gcgcgctctc catcgcgtag cccagcaaca tgcgcgtcag gccaaaccgc caggtgtgtt
    13681 gaccggtagc gggaagttcc agctcgcgaa cgttgtcgtc atctatcccc caacgaatgc
    13741 cggactcgtt gacccactgg cgtaaataac gcagcccttc ttcggtgatg tcaaaccgcg
    13801 ccgccagcac cggcacatcc agtaatgcca gcacgtcttc cgacacaaag cggctgtctg
    13861 gcaacgataa caggctgata aacgcttcaa gtacaggatg cgactgccgc gcccgacggt
    13921 cggaaatggc gtaaggcagg taacgatccg caggtgcgct accaaacaca gcctgaataa
    13981 acggactgta gctgtcgata tcagccacca tcacgatgat gtcgcgcgga gtaagtgtcg
    14041 ggtcttcctc cagcatcgcc agcaggcgat cgtgtaaaac ttcaacttca cgctgcgggc
    14101 tatggcaaac gtggaaggtg atactgctat ccagtggatc aagcgggcgt ttgttatcgc
    14161 tacgggaaaa ctcttcgatg ttcacaccag caacggcgcg gttttccagt tccagaatgt
    14221 cagactgaat attatgcagc aggttatctg gcgtcacatc gacaaaagcg tccagctcct
    14281 ggctgctctc caggtcagaa aggagataaa tgtagtcgcg cccaagcttg ccccatgaag
    14341 ccagcagcgg gttgccgaca tcctgttcac catcgctgtt aaagagctgc ccggcatttt
    14401 cgctgtcgcg aaatagcggt aattcgcgat cttcaaaact gtgtcggcgc tggcgagtca
    14461 gtagtttcgc cagataagcg ggatctttaa tgtcgcccca gtaataacgg caggggttgg
    14521 taaacaggag atggatttca atatgtttac ccagcgcctg tagcgcctgg agataaacag
    14581 gcggtaacgc ggaaataccg catataaaga cgcgcgaagg taaccccggc gggcaggtcg
    14641 tcgcggactc cagcgtttcg ataaagcgct gatagagatt ggcgcggtgc cagcgcggtt
    14701 gcccaagttc gtcggtatat tccaccaacg ccttccacaa cggcgcttgc caggcctgtg
    14761 cttctcccaa cccttctacc agatgtcctg tttcccactg tgccagccag tccggacgat
    14821 agaccagata ctggtcaaac aggtccgccg cttttgaaga aagctggaac agttttcgct
    14881 tgtcactatc gtcagtcaga taatgccgca acagggtaaa gtcttcgcgc tccaacagtt
    14941 gcggcagcag tgtcatcagt ttccagctca tgctctgttt gttaaaggcg ctctctttgg
    15001 ggatctccgg taacacccgg acgaacatat cccagataaa gctcgctggc agcggaaaat
    15061 caatgtttgc cgcaatacca aacttttgcg acagggtcat ttgcagccac tgtgccatac
    15121 cggtactttg caccagaatc atctctggtt cgaaaggatc gtccagccgt tcgcgttcga
    15181 caataaactc catcaacgct tccagcacgt ccagacgatt ggaatggtag acccttaaca
    15241 tagcggctcc tgactactga cgattcgggc aatgcaggcg ggtcatctcg ccttctctgc
    15301 ccccgggtga aactagcgta acgctgatgc tgacacatcc cgcctgcgat gtctgcattc
    15361 ggttgacctg ccagttagca ggtggcgaaa tcgcgcgcag ttgcgtttgc tgccagccat
    15421 gccgccagag ctgttggtac tggtttctgc tggcaagact gttcattaat gttcgctgga
    15481 aacccgataa cgcagtgaca atcatcacca tcaacaccat cgccaacatt acctccggca
    15541 gactaaagcc ttgttgattc ctcagggaag ctgacataac gccctctctt tcagcggaca
    15601 aaaatcgctc cagccgcgtg gcgaaaaaac aatgtttcca tcgatgactt cgcctgttcg
    15661 ccacaacgaa acgccttcat aaccagcaat caataaggct tcattatctg ccagtaaacg
    15721 cagacaaacc cgggcaccgg tggcagcata cagtaagcac tgaactgcgg gcagcgtctg
    15781 ccaggagtgc atttttcccc actccagcgc cgactgaacg atggcctggc ggcgcaatga
    15841 ctgactttcc atgctcacgc gagaagcaaa actgcgatcc tgctgactca ttccttgcaa
    15901 tagcaagcta cccaaaatca gcagcatcag gaccagagcc agtgacgaaa cacccttttc
    15961 gcggttcaca ggttggatcc tgtcacgcta tagctggcat ccaccacggt ttgcggatca
    16021 gccttgctgg cagcgtgcat attaaccgtc aacaccggcg agaagccgct gacatcctga
    16081 cgtacgacct gaaaagtgtc gataatgatg gcatccggat tagtgacttt atcccagccc
    16141 ttaccttcac aggatgtcgc accgcgtagc gtttccagca catgctcctt cagacgaaat
    16201 ccaatctggt cggactcttt taccggttcg cgatcccaga taccattact gttcgcatcc
    16261 cactgcacaa tgacacagtc accctgtccg acaatttcca gcccttcgcc cgtacagctg
    16321 ccatggcaat aacccgccct ctggagatgc ttcgcgacgg taaatacccg cagccagatt
    16381 tcatcttcca gcgccagctt acgggtgttc gttaaacttt cacgctgtaa cgcaggcaga
    16441 aagcgtgcag cccccagcaa caatacgcta ctgatcgcca tagcaatcaa cacttccagc
    16501 agagaaaaac cttgctcttt tacaggcatc cttctgtttc tccttgctga caaagccgga
    16561 gtcttcccca cggcgaaacc actagccacc actcgcccgt tgagtttttg aagcgaatat
    16621 gcccggccca tgcggtattg cgcaggccaa agaaagcaag cgaaggtgtc aggtcgctca
    16681 tttcgacttc gggccagcgc ggcacaaaga ccaatggtga actgccatga caggtattcg
    16741 ccccagcaac ggaactcaca aggcaccata acgtcccctc cctgataaga ctgatactgt
    16801 ggtcgcggtt atgccagttg gcatcttcac gtaaatagag caaatagtcc cgcgcctggc
    16861 tggcggtttg ccaaagccgt tgcgactgct gccagtattg ccagccatag agcccacttg
    16921 cgcttagcat gaccagaatc agcatcgcga ccagcgtttc aatcagcgta taaccacgtt
    16981 gttttttcat gccggcagta tggagcgagg agaaaaaaag acgagggcca gtttctattt
    17041 cttcggcgca tcttccggac tatttacgtc gttgcaggac gttgcaaaat ttcgggaagg
    17101 tgtctcgaag aatttaacgg agggcaaaaa aaaccgacgc acactggcgt cggctctggc
    17161 aggatgtttc gtaattagat agccaccggc gctttaatgc ccggatgtgg atcgtatcct
    17221 tcaatctcaa agtcttcgaa acggtagtcg aagatggatt cgggtttacg tttgataatc
    17281 aacttcggca gcggacgcgg ttcgcggctt aattgcagat gagtttgatc catatggttg
    17341 ctgtacagat gcgtgtcgcc accggtccag acaaaatcac ccacttccag atcgcactgc
    17401 tgcgccatca tatgcaccag taacgcgtag ctggcaatgt tgaacggcag gccgaggaag
    17461 acgtcacagg agcgctgata aagctggcaa gagagtttgc cgtctgccac atagaactgg
    17521 aagaatgcat ggcacggtgc cagcgccatt ttatccagtt cgcctacgtt ccacgctgaa
    17581 acaataatgc gacgggaatc cgggtcgttt ttcagctggt tcagtaccgt agtgatctgg
    17641 tcaatatgac gaccatccgg cgttggccag gcgcgccact gtttaccata cactggcccg
    17701 aggtcgccgt tttcatcggc ccattcgtcc cagatggtga cattgttttc gtgtagataa
    17761 gcaatgttag tgtcgccctg caggaaccac agcagttcgt ggatgatgga acgcaggtgg
    17821 caacgtttag ttgtcaccag cgggaatcca tcctgcaggt taaaacgcat ctgatgacca
    17881 aaaatggaaa gcgttccggt tccggtacgg tcgtttttct gtgtgccttc gtcgagcact
    17941 ttttgcatca gttctaaata ctgtttcatg gttcctcagg aaacgtgttg ctgtgggctg
    18001 cgacgatatg cccagaccat catgatcaca cccgcgacaa tcatcgggat ggaaagaatt
    18061 tgccccatgc tgatgtactg cacccaggca ccagtaaact gcgcgtcggg ctggcggaaa
    18121 aactcaacaa tgatgcgaaa cgctccgtaa ccaatcagga acaagcctga gacagctccc
    18181 attgggcgcg gtttacgaat atacaggttg aggataataa acagcaccac accttccagc
    18241 agcagctcgt aaagctgtga tgggtggcgc ggcagcacac cgtaagtgtc gaaaatggat
    18301 tgccactgcg ggttggtttg cagcagcaaa atatcttctg tacgggagcc agggaacagc
    18361 atggcaaacg ggaagttcgg gtcaacgcgg ccccacaatt caccgttaat aaagttgccc
    18421 agacgcccgg caccaagacc aaacggaatg agtggagcaa taaaatcaga gacctggaag
    18481 aaggaacgtt tagtacggcg ggcgaagata atcatcacca cgataacgcc aatcaggccg
    18541 ccgtggaagg acatgccgcc gtcccagaca cggaaaagat acagcggatc ggccataaac
    18601 tgcgggaaat tgtagaacag aacataacca atacgtccac cgaggaagac gccgaggaag
    18661 cccgcataga gtaagttttc aacttcattt ttggtccagc cactgcccgg acgattcgcc
    18721 cgtcgtgttg ccagccacat tgcaaaaatg aaacccacca gatacatcag tccgtaccag
    18781 tgaagcgcca cgggtcctat tgagaaaatg accggatcaa actccggaaa atgcagatag
    18841 ctactggtca tctgtcacca caagttcttg ttatttcgct gaaagagaac agcgattgaa
    18901 atgcgcgccg caggtttcag gcgctccaaa ggtgcgaata atagcacaag gggacctggc
    18961 tggttgccgg ataccgttaa aagatatgta tatgatccgc gctataaccc cccacgaatc
    19021 agcccgccca tgccgcgacg ctccataaag gctgcaacct gatggcgaac ttcggtcgcc
    19081 agttgcgctt ccagactacg ctgcgcaaga ttttctgctt cggcaaaatc aatgcgccgc
    19141 agcaggtatt ttacgcgcgc tacagaacgt ccgttcatag acagatggcg atacccaagc
    19201 ccaatgagga ttgccacgca catcggatcg cccgccattt caccgcacaa acggagatcg
    19261 attccatgta tttccgcttc ccgggcgatc atcgccagag ctcgtaacat tgcaggatga
    19321 agactgtcat aaatgttcgc cacccgggta ttgttgcgat caacggccag gatgtattgc
    19381 gtcagatcgt tggtgccaac agagatgaaa tcgacccgct ttgccagatg cggcagcata
    19441 aacaccattg acggcacttc cagcatgatg ccgatacgtg gtttgggaat ttcgtaaccg
    19501 atcatctcct cgacttcacg tccggcacgt tcaatcaggc ggcgtgcttc gtcaacttca
    19561 tcgaggcttg tgaccatcgg caacagaata ttcaggttgc ccgtagcggc attagcacgc
    19621 agcatcgccc gcacctggat caagaagatc tccggctgat cgagcgtaat gcgaatccca
    19681 cgccaaccca ggcatggatt ctcttcgctg atcggcatgt aaggcagctg cttatctgct
    19741 ccgacatcca gcgtacgcaa ggtgacgggt ttatcattaa acatttgcag catcccctga
    19801 tactgcgcca cctgttcttc ttccgacgga aaaccacttt gcagcatgaa tgggatttca
    19861 gtgcgataaa gtcctatgcc atcaatacgg ctgcccagtt tttcttcatg ttccgggctt
    19921 aaaccggcat tgagcatgac tttaatgcgt tcgccgcttt ttaactgggc gggtaaattg
    19981 acgtcatctt ccgccagacg gctaagctcg atctcttcac taattagccg ctgatattct
    20041 tgcagcagta ccggctccgg atcgaccagc aattcaccgc gataaccatc gacgatcagc
    20101 gtccgacgat gcagtaccga aggctgaata tccgcgccca tcacggtagg gatccccagc
    20161 gcacgtacca tgatcgcagc atgggagttg gcagcgccat cgcgcacgac aacaccgact
    20221 aagcgatcct ggggcagctc agcaagcgtt gtcgctgaca gttcatctgc caccagaatg
    20281 aaacgttccg gccaggcgtt cggcccttga ttagcgtcat caagatgaaa cagcaatcgc
    20341 tgaccaagtg cacgtaaatc gccagcccgc tctttgagat agttatcgct tagcgcggca
    20401 aactgttcgg caaatttttc aatgaccgtt tttaccgccc actctgccac cgagccttta
    20461 tcaacctcgg caaacaattc gcgacgcagc cgggtatccg aaagcaggtg tgagtaaaga
    20521 tcgaaaatag ccgccgtttc tttttgtgca ccggcagcaa agcgtttgct atagcggcga
    20581 aactcgttag ccgcctcttc caacgccccg gtcagtcgtt cgcgttccag agccggatcc
    20641 agcgttgatg cctgatacac ctgttccatt aaaggtaacg tggcatcctg ccagccttcg
    20701 gcaatcgcca caccaggtgc tgccggtaat gcacggattc gcgtctggcg atattgccca
    20761 aacaaggcag tcaactgcga ctgagaaaga atagctgcca tctgggtggc aagcgtcacc
    20821 aggaaggatt cttcactttc gtcatactgg cgcaactctc gttgctgtac caccagtaca
    20881 ccaagcaact ggcgacgttg aataattggt acgcctaaaa acgcgcggaa acgttcttct
    20941 tttacggagg ggatgtattt gaagctgggg tgcttttgcg catctgcaag gtttatcggt
    21001 tccgccagcc tgccaaccag gccgacgatc ccttcatcaa acgcgagcgt tacagtgcga
    21061 ccgcgtggtt ttttcagtcc ccgggtcgcc atcaggtagt aacaacgtcg atcatgatcg
    21121 gccaggtaga ccgaacagac ctcggtatcc atcgcaagac agatgtcggt aaccagaata
    21181 tttaacgcct cattcaggcg tggtgcgctg gctacctttt cgactatttc gcgcaggcga
    21241 gtgagcataa ttggcgtgac ttaacctctt ttacgtcgat aagcagatgc gttttgtggt
    21301 tttggcgtat tttcctgcag tgacatcacc acactcgcga actctttcat taccctacgg
    21361 tagacatcac gtttaaatga caccacctgt ctgactggat accagtaact tacccatcgc
    21421 cagccgtcaa actctggtgt actgctggtt tgcatattga tttctgcatc gccgctcacc
    21481 agctgcaaga gaaaccattt ttgtttttgg ccgatacaaa ccggcttcgt gtcccaacgc
    21541 accaaacgtt tcggtaattt gtagcgcaac cagttacgcg ttgaagcaag gattcgaacg
    21601 tctttgcggc ttaatcctac ttcttcaaac aattcacggt acatcgcctg ctctgcggat
    21661 tctccggggt tgattccgcc ttgcggaaat tgccaggagt gctgaccaaa tcgccgggcc
    21721 cacattacct gcccctggcg attacaaatc acgataccta cgtttgggcg gtagccatcg
    21781 tcatcaatca ccggactacc tcaaaataaa gctttatata cgaatgattg tttcatactc
    21841 caggaagacg gtaaaccact ctctgcaggg cattacacac taataacaat tgaataactc
    21901 acagttatgt gcagagttat aaacagagga aggggtggat agccgttttt attcaccttt
    21961 tctgtggata gagttgtgaa gaactacgga attactacgg gaaaacccgg agcattctga
    22021 ataagacaga tatagagatt ttaaaatatt cattatcagt caatgtatta catgtgattt
    22081 gagtaataaa aaagagtaat tcgtgaccca ggtcacacct ctcatttacg ggttggcgaa
    22141 agatcaacca acgccgtatt tatccacaga atgtgccact aagttaagca ctgaaccact
    22201 aaaaactgga gtttcgtcgc acgtcaaggc tgtaaatgga aacagtagtg aaggtttttc
    22261 acagttatcc cagctttctg tggataacat ggtgtaagat cctgtttatt ttcagtgacc
    22321 agatttggaa aacccgttgc agtgttgcgc aactcgatta ccggcaacct aaaaagcaat
    22381 ataaatcaga gaattgaaca acgcatgtgg aaaaagttac actgcgaata ttcggcacat
    22441 aattgctgtt tgttttttaa ccaaggtatc atgacatgtc ccaacctcgc ccactgctct
    22501 ctcctcccga aactgaagaa caattgttag cgcaagcaca acaactttct ggttatacat
    22561 tgggagaact ggcggcactt gccgggctgg ttacgccgga gaatttaaaa cgcgataagg
    22621 gctggattgg cgtgttactg gagatctggc taggtgccag cgcagggagt aaacctgagc
    22681 aagattttgc tgctctgggc gtggaactta aaactatccc tgtggatagt cttggtcgtc
    22741 cgctggaaac aacattcgtt tgtgttgccc cgttaacggg caatagcggg gtgacatggg
    22801 aaaccagcca cgtgcgccac aagctcaaac gcgtgctgtg gataccggtt gaaggcgagc
    22861 gcagcatccc gctggcaaag cgtcgcgtag gatcaccgtt gctgtggagc ccgaatgaag
    22921 aggaagaccg gcagctacgc gaagactggg aagaattaat ggatatgatt gttctcggtc
    22981 agattgagcg gatcaccgct cgtcacgggg aatatttaca gatacgaccg aaagcagcga
    23041 atgcgaaagc gctgaccgaa gctattggtg cccggggcga acggattctg acgctgccgc
    23101 gcggatttta tttgaagaag aatttcacca gtgcgctact ggcccgtcat tttctgatcc
    23161 agtagccatc gctttgacct gccgctttcc gggcatataa ttaccgcttc atttttttgg
    23221 cagggctttt tagatgttat ttgcatggat aaccgatcct aacgcctggc ttgcgctcgg
    23281 tacgctgacg ctgctggaga tcgttcttgg gatcgacaat attattttcc tttctctggt
    23341 ggtggcaaag ctcccaacag cacaacgtgc tcatgcgcgc cgtctggggt tggcgggagc
    23401 catggtcatg cgtctggcgc tgctggcatc catcgcctgg gttacgcgcc tgacgaatcc
    23461 gctttttaca atattcagtc aggaaatttc cgcccgtgat ttgattctgc ttctgggtgg
    23521 cttgttcctt atctggaaag ccagcaagga aatccacgaa tccatcgaag gtgaagaaga
    23581 agggctgaaa acacgcgttt catcattcct cggcgctatc gtgcagatta tgctgctgga
    23641 tattatcttc agcctcgact cggtgattac cgctgtgggt ctgtcagatc acctgtttat
    23701 tatgatggct gccgtggtga ttgccgtagg cgtgatgatg ttcgccgcgc gctcgattgg
    23761 tgattttgtc gagcgccatc cttcggtaaa aatgctggcg ctctctttcc tgattctggt
    23821 gggctttacc ctgattctgg aaagtttcga catccacgta ccgaaaggtt acatctactt
    23881 cgcgatgttc ttctctattg cggttgaaag cctaaacctg attcgcaaca aaaagaatcc
    23941 gctctgatac tccgtacgct ctcctgcggg agggcgtact cactcctgtt accctcacag
    24001 tttaagattt tcctgctttc aagatatata acgtcggttt ataaacagac tattatcata
    24061 ggtgggccaa atgaggataa ccggatgaaa aaatgggcag taataatttc tgcagtcgga
    24121 ctggcgtttg ctgtttccgg gtgttccagt gattacgtca tggcgaccaa agatggccgt
    24181 atgattttga ccgatggaaa acctgaaatt gatgatgata ccgggctggt gagttatcac
    24241 gatcagcaag gtaacgcgat gcaaattaac cgtgatgatg tttcgcaaat tattgaacgt
    24301 taacaaataa ggtcagcatc cggctggcct taagattttt ctctttccct tttcccttcc
    24361 ctctgccatt tttatattcc ttatgtcgtg attataaaaa ggaaacggct atgcaatatc
    24421 accgtatacc ccacagttcg ctggaagtca gcacgctggg gcttggcacg atgacgtttg
    24481 gtgaacagaa cagcgaagcc gacgcccacg cacaactcga ctatgccgtc gctcagggca
    24541 ttaaccttat cgacgttgcc gaaatgtacc cagtacctcc gcgccccgaa actcaagggc
    24601 taaccgaaac ctacgtcggc aactggctgg cgaaacatgg cagccgcgaa aagttaatta
    24661 tcgcctccaa agtgagcgga ccgtcgcgca ataatgacaa aggcatccgc ccggatcagg
    24721 cgctggatcg gaagaatatc cgtgaagcgc tgcatgacag cctcaagcgt ctgcagactg
    24781 attacctcga tctctatcag gtgcactggc cgcagcgccc aaccaactgc ttcggcaaac
    24841 tcggttatag ctggacagat tctgcgcctg cggtttcgct gctggatacg ctggacgcac
    24901 tggcagagta ccaacgcgcg ggaaaaattc gttatatagg cgtgtcgaac gaaactgcat
    24961 ttggcgtaat gcgctacctg catctggcgg acaaacacga tctgccgcgt attgtcacca
    25021 ttcagaaccc ctacagcctg ttaaaccgca gttttgaagt aggtctggca gaagtcagcc
    25081 agtatgaagg ggtcgaactg ctggcctatt cgtgcctggg tttcggcacg ctgaccggga
    25141 aatatctcaa tggtgcaaaa cccgctggcg cacgtaatac gctctttagt cgcttcaccc
    25201 gctatagcgg tgagcaaacg caaaaagccg tcgcggcgta tgttgatatc gccagacgtc
    25261 atagcctgga ccctgctcag atggcgctcg catttgtacg ccgtcaaccg tttgttgcca
    25321 gcactctgct gggcgcaacc acgatggagc agctgaaaac taacgtcgaa agtttgcatc
    25381 tggagttgag cgaagacgta ttagctgaaa ttgaagcggt gcatcaggtt tatacgtatc
    25441 ctgcaccata agattaacgc cggttaaaac cggcgttaaa tattaatgac ggcgctgcca
    25501 gatccacagc gccgttattg ccagcgcaaa cagcgcaccg aagccaatgc caatgggcac
    25561 gaccgggatg cctaccatta ccgccagcga gtaaatgccc agcatcaaca acatggcgct
    25621 gttttcgcca aggttttgta ctgcaatcgc attccccgcc ccgacgcttt ttttaccccg
    25681 ctcctgtagc aacgcattga gcggaacgac aaaaaaaccc cccagcacgc caatcagcat
    25741 caacaaagca taggctggca gcagctcgtg ttgcagggag aaaatcagta ccaccacgcc
    25801 aatcaaaatc ccggctggca tacagcgtga cacggtttcc agcgtaacta acttcgctgc
    25861 cgcacctgca ccaaccacga taccaatcgc taccatcgcg ttgagatagg tcggcgtagc
    25921 gttatcggta atacccagcg ccaccggtac ccacagcacc aacaggaaac gcagcgtgac
    25981 acccgctccc cagaataaac tggtgcccac cagcgaaaaa cgcgtttcac cattgcgcca
    26041 tagcgaggtg caggcattca ggaaactgcg ggtcatgctg atgagattcc aggactgccc
    26101 cggacgcgcc gccgccagtt tgggaatgta gatattggca atgaccgcgc caccgtaggc
    26161 cagtgcgcat gcggccaggg cgatcaggac atgccagtca gccagaacac caccagctac
    26221 ggaaccgagc aaaatcgccg ctatggtaga cgcctccatt aaaccgttag cttttactaa
    26281 cttactaccc gtggttaatt cgccgagaat accgtatttc gccggtgaat aggctgcagc
    26341 accaacaccc accaacgtat agccgagaaa cggattgata ccaaagcaga tactggctgc
    26401 gcccagtagc ttcaggccgt tggcaaacat catcacccgg cctttggcga agctatccgc
    26461 cacctgcccg acaaacggcg caaaaagaat gtaagcacct acaaacacca tttgcaggat
    26521 gggctggctc cactccggat agaactgcgc tttcagtaac gccagagtag caaacagtag
    26581 cgcattatcg ccaaacgcag agagaaactg cgccacgata accgctttca tccccttcga
    26641 ccacaacgaa gtgttagtgt gcactgactc actcatcgtg ttgttccgct tcgtctaccc
    26701 agcttttcaa cgtgacaaag tcaggtttgc cgctgccaag taatggcatc tgtttcagat
    26761 agcgaatatc gcgcggtaca gcaagctccg gcacgccgtg ctcgcgggca tactgttgta
    26821 acttatcgcg cgtcagttcg ttatctgtgg tgaaaagcac cagtgcctcg cctttgctgg
    26881 catcgctctt aatcgcagtg gcatggactt tatctggcga aacaccaagt gccagttgtt
    26941 ccaccatttc cagcgacacc atttcgcctg caattttggc aaagcgtttt gcgcggccct
    27001 gaatctgcac aaagccctgc tcatcaaaac gcacgatatc gccagtgtca taccagccgc
    27061 gctccatttc gccgcgaata ttctcggcgg tgggcacttc cagtacacca ggcttctcca
    27121 cccgcagata gccgttcatt atgttcggcc ctttcagttg caggcgtccg ccctcttcga
    27181 taccaggaac cgacaacagg cgcgcatcca ttcctggtag aatacgccct accgtaccgg
    27241 gtttcgccgc catcggtacg ttgatagaaa cgacaggcgc gcattcggtc acaccgtagc
    27301 cttcaaggat gcgcaggcca aatttatcct gccaaagctg tttggtactt tcttgtagtt
    27361 tttctgcgcc tgccaccaca tagcgtagac gatagaagtc atacgggttg gcaaagcgcg
    27421 cgtagtgacc gaggaaagtc gaggtaccga acaacacggt gcaactgcgg tcatacacca
    27481 actccggcac aatgcggtaa tgcagcgggc ttggataaag gaacacttct gcacctgtga
    27541 gcagtggcgt aaacaggcct accgtcagcc caaaggagtg aaacagcggt aacgccgaca
    27601 taaagcgatc gttggtggtg aagtcggcaa tcgttttaat ctgctcaacg ttcgccagaa
    27661 tgcttttatg gctatggacg acgcctttcg gatggccttc agaaccggag gtaaagagga
    27721 tcagcgcttc ttcttccggc tgctgtttta actgtgccag acgcggcatc agcaaatgag
    27781 cgaaaatcca cactttgtcg gcagtggtga catctgcttt taaatcttcc agatagaccc
    27841 agcgcacctg agtaagttgc tccggcagat gccagagttt gcctttatcg agaaactgac
    27901 gggaagtgaa gatggttttg atttcagccg ccgtaatagc actggtcagc ccttttaccc
    27961 cggcggtgta gttcatcatt gcgggaatgc ggcgacgggc gatggcccca aaaatcactg
    28021 ccgcactgat gcctgcattg ggcagcatta agccgatgcg ttcgccttca acactgtatt
    28081 tttcaaggat gcgtccaaca aacagcgttt tcgtaagcaa tttgcgatag gagtctgggg
    28141 taaagttgac gtcttcgaca catttcttcc cggctccgaa gcggtacatt gcgctcagta
    28201 aagattcgta cagcgtttca cgcgggcgca ccgccatacg cgcctccatc attatttgat
    28261 gcagcatttc gccagcgatt ttgcgacggt cacgggcacg cggcgcatcc ggcatctcca
    28321 cctgcgttgg tggcaaaata tgcagagtaa tttgcgggaa caagcgacgt ttaaccagac
    28381 ctttcaggcg gctgaagtgc gtaagttccg ccccttcaat acgcacagga ataaccgttg
    28441 caccagactt cgccgcgaca aatcccgcgc catcgtagat tttcatcagc gagccagtgg
    28501 tggtgatgcg tccttcaggg aaaatcacca ctggtcggcc ctgttccacc agacgtacca
    28561 gatgtttaat cgccataggt tgcgtcgggt cgagaggaac aaagtcgata aatgatttca
    28621 gccaacgcat ataccactgt tggcttattg aggtgtaaac ggcaaatacc ggacgtacgg
    28681 gtaaaaacaa tgccagcagt atgccatcaa taaaagagac gtgattaggc gtgattagaa
    28741 cgcgctcgcc cttcagtgcc ttggtgtcac ccgtaacgcg aacgcgatac aaaacacggc
    28801 acaaatttcg aaaaaagcta aaaagcattc caactccctt tgctctgatt cagtaaaagc
    28861 gaatggaggg agattacacg agataaagaa cgcgagcgac agtaaattag gtgcgaaaaa
    28921 aaacctgcgc atccgcgcag gttggtgcaa gagacagggt acgaagagcg taccgaataa
    28981 tctcaccaat caatacctct gggatcttga ttgtggtctg cacgacgact cttcgccagc
    29041 gagaaaacgc aaaggaatga agggaaatgc aacgaggtgt gtaaattgtc ggttactgtt
    29101 acagattgat gaccggcaaa aaaaaacctg cgcatctgcg caggctggtg taattcatgt
    29161 gctcaacccg aagttgactt cacctatcaa tacctctggg atcaccactt tagcaacctg
    29221 aagccaaacg ccaccagcgg tcaatcgcaa cagcgtttcg caaagtgtaa ccaaaggttt
    29281 gcaatctctt tttttgtctt acttatttgc gtttggctca cattcccaca atgaaaacac
    29341 gccgcccctt gaaccaacgg gcgttttccg taacactgaa agaatgtaag cgtttaccca
    29401 ctaaggtatt ttcatggcga ccataaagga tgtagcccga ctggcaggcg tttcagtcgc
    29461 caccgtttcc cgcgtcatta ataattcacc caaagccagc gaagcttccc gactggctgt
    29521 gcatagtgca atggagtctc ttagctatca cccgaacgcc aacgcccgtg cgctggcgca
    29581 gcagaccact gaaacgatcg gtctggtcgt tggtgatgtt tccgatccgt ttttcggtgc
    29641 aatggtgaaa gcggtcgaac aggtggctta tcacactggt aattttttat tgattggcaa
    29701 cggttaccac aacgaacaaa aagagcgtca ggccattgag caactgatcc gccatcgctg
    29761 tgctgcgctg gttgtccatg ccaaaatgat cccggatgcc gatttagcct cattaatgaa
    29821 acaaatgccc ggtatggtgc tgatcaaccg tatcctgcct ggctttgaaa accgttgtat
    29881 tgctctggac gatcgttacg gtgcctggct ggcaacgcgt catttaattc agcaaggtca
    29941 tacccgcatt ggttatctgt gctctaacca ctctatttct gacgccgaag atcgtctgca
    30001 agggtattac gatgcccttg ctgaaagtgg tattccggcc aatgaccggc tggtgacatt
    30061 tggcgaacca gacgaaagcg gcggcgaaca ggcaatgacc gagcttttgg gacgaggcag
    30121 aaatttcact gcggtagcct gttataacga ttcaatggcg gcgggcgcga tgggcgtgct
    30181 caatgataat ggtattgatg taccgggtga gatttcgtta attggctttg atgatgtgct
    30241 ggtgtcacgc tatgtgcgtc cgcgcctgac caccgtgcgt tacccaatcg tgacgatggc
    30301 gacgcaggct gccgaactgg ctttggcgct ggcggataat cgccctctcc cggaaatcac
    30361 taatgtcttt agtccgacgc tggtacgtcg ccattcagtg tcaactccgt cgctggaggc
    30421 aagtcatcat gcaaccagcg actaaccgca gttaaagcaa ttccagcgcc agtaattctt
    30481 caatggtctg gcgacggcga atcaaccgcg cctgaccatt atcaaacaga acttctggta
    30541 acagcggacg gctgttgtag ttggatgaca ttgacgcgcc atatgcccct gtatcatgca
    30601 gtaccagata atcacccgcc ttcacttccg gcaaggcgcg ggtttcgaca tttccccctt
    30661 cctgctgggt aaagacatcg cccgattcac ataacggccc ggcgacgacg gtttccaccg
    30721 ttggcgcgtg ttccagagaa cgaccatcag ctgccagggc actgatatgg tggtaactac
    30781 cgtacattgc cgggcgcatc agatcgttga acccggcatc aaccagaaca aagtggcggc
    30841 tacccatttg tttgacgctc cgcacttgag taattaatac gccagcctgc gctaccagaa
    30901 agcgacccgg ttcaatttcc agtttcacag gatggcccaa atggcgggcg atttgctcac
    30961 gcgcggcatt ccacagacca taataatgtt cggtatcaac cgcctcttca ccctgttgat
    31021 aaggaataga aagcccaccg cccgcagaaa tagcctgtaa atcctgaccg aattcgagga
    31081 cttgacgcac catagcacca catacctgtt ccagatgggc ataatcgacg ccagaaccaa
    31141 tgtgcatgtg aatgccgacc agctgtagat gatgacgttg tatcacgtcc agtgcggcgg
    31201 gcagatcggt gtaccagata ccgtgcttgc tgttttcgcc accggtattg gttttttggc
    31261 tatgcccgtg accaaacccc ggattaacac gcagccatac ccgatgccct ggcgaaacct
    31321 gccccagttg gtcgagcata tcaacagaac ccgcattcac cggaatttgc aattcactga
    31381 cgcgttcaag cgtcgcctga tcgataacat ctgccgtaaa aacaatatca tcagggtgcg
    31441 tttgcggatt gtaacccgcc gccagtgcac gctctatttc acctaacgag acggaatcca
    31501 cttttacgcc ctgctcacgc attaagcgca aaatatgaat attggaacag gctttctgtg
    31561 caaagcgcac cacatcaaac tgtttcagcg ccgcaatctg ccgacgaata atttgcgcat
    31621 cgtagaccca caccgggcag ccaaattctg cgggcaaacg cagcagattt tcggcggtga
    31681 gatcggtatc agtgctgaac agtgaatgtg gcataacaaa ctccagataa gtgctttttt
    31741 atgattacgc cacatcataa aaagaataaa aaatatcgat ttatgtcgag tctatgcaaa
    31801 aatgatatgg attaccggat tgcgagagag cgctaatggc cgccgttaac ttacgtcata
    31861 ttgaaatttt tcatgcggta atgaccgccg gaagcctgac tgaggcggca cacctgctac
    31921 acacctcaca gccaaccgtc agccgcgaac tggcgcgctt tgagaaggtg atcgggctga
    31981 aattgtttga gcgcatacgt ggacgattac atcctaccgt gcaaggactg cgtctgtttg
    32041 aagaagtgca acgatcctgg tacggactgg atcgcattgt cagtgccgca gaaagtctgc
    32101 gcgagtttcg ccagggagaa ctgtctattg cctgcctgcc ggtcttttcg caatcttttt
    32161 taccgcagct cctgcaaccc tttctggcac gttatcccga tgtcagctta aatatcgtgc
    32221 cccaggaatc accgctactt gaagagtggc tctcggccca gcgtcatgat ttaggactca
    32281 ctgaaacgct ccatacgcct gcgggaacag aacgtaccga attactctct ttagatgaag
    32341 tgtgtgtgtt acctccgggc catccgctgg cggtaaaaaa ggtattaacg ccggatgatt
    32401 ttcacggtga gaactacatc agcctttccc gtactgacag ctatcgccag ttgctggatc
    32461 aattgtttac tgagaatcag gttaaacgac gcatgatcgt agaaacccac agcgccgcgt
    32521 cagtctgcgc aatggtacgg gcgggggtag gcgtttcagt ggttaacccg ctcaccgcac
    32581 tggattatgc ggcaagcggt ttagtggtgc ggcggttcag cattgcggtt ccattcaccg
    32641 tcagcctgat ccgccccctg caccgcccgt catcagcgct ggtgcaggcg tttagtgagc
    32701 atttacaagc gggattaccg aaactggtca cttctcttga cgctattttg tcgtcagcta
    32761 cgacagcata aaagcgacag catcctcggc atggatcgcc gcggtatcaa acacaggcag
    32821 aacactgcgc tcttctggca ccagtaaacc aatttctgtg cagccaaaaa tgacgccctg
    32881 tgcgccctgt tctgcaaggc gagcaatcac ttgcgcataa taagcgcgtg acgcttcggt
    32941 aaattgcccc agacacagtt cttcaaaaat aatctgatta attttcgccc ggtcatccgc
    33001 ttcaggaata agacagttga tggaaaattg ttccgtcagc cgcccgcgat aaaaatcctg
    33061 ttccatggtg taacgtgtac ccagcagcgc cacacgagtc attcctgccc cggtaattgc
    33121 acgtccagtg gcatccgcta tgtgtaagaa aggcagagag caacgagact caatggcatc
    33181 cgccactttg tgcatcgtat tggtgcataa cacaatacct tctgcgcccg cccgctgtaa
    33241 gccaagcgcc gcctcagcca gaatatcccc ggttttgtcc cactcaccgc gacgctggca
    33301 ctcttctatt tcatgaaaat cgacgctatg tagcagcact tgcgcagagt gaagcccacc
    33361 aagccgctgt ttaatgcctt catttatcag acgatagtaa ggaatagtgg attcccagct
    33421 cattcctccc agcaaaccaa ttgttttcat tcctctctcc tgatgagtgt taccgcaaca
    33481 aggctgaata aggaacgagg caaacgcctc aacggccagg tgcctaatct ctattaacga
    33541 aaaaagggcc ggatgtacag cacatccggc ccgtgaaatc agacgccgat atttctcaac
    33601 ttttcgcctg ccatcagttt gcgctcgata tgttccagcg tgacattttt ggtttccgga
    33661 atgagccaga aagtgatgcc cacaaacgca atgttcagcg cagtgtagag ccagaacgta
    33721 ccggcagcgc caatgctatc aagcagtgtc aggaaggtcg cgccgataat catattcgac
    33781 acccagtttg tcgtcgttga gcaggtgatg ccaaaatcgc ggcatttcag cggctgaatt
    33841 tcagagcaca agatccacac cactggcgcg gcgctcatcg cataaccggc aatacacatc
    33901 atcgtcatgc caacagagag ccaggacaag ccactggaag ccgtaccgtt atcaaactgc
    33961 atcaggcagt aacccagcac cagagtgcct aacgccatca cgctgaaacc aattttcagc
    34021 gccggcttac gccctgcttt atctaccgta aagaccgcaa tgaaggtagc gaacataaag
    34081 gtcagcccca cgaccagagt cgcaatcatc tgttgttctg tggtcgtaaa gcccgccatt
    34141 ttgaagatac gtggcgcgta gtacatgatg atgttcatac cggtaaactg ctgcatcgcc
    34201 tgcaacaaca taccgagaaa tacagcacga cggacgttac ggttgatctt aaacagtgcc
    34261 caaccgccct gttttaactt caggctttcg cgaatttcgt tgagttcttc tcgcgctttt
    34321 tccgacgtat cgcgcagcat acgcaatact tcttccgcct caatatgacg ccccttttcc
    34381 gccagccagc gcgggctatt tggcaggaag accaccagaa taatcagcag aactgctggt
    34441 aaagcaagaa cccccaacat tgcgcgccag ttaccgctat aactgaacgc tgtatcggat
    34501 aaaaacgcca gcacgatgcc gagagtgacc atcaactggt acatgctgat catcttaccg
    34561 cgaacgtttt cacttgccat ttcagaaaga tatagaggag cggtgtaaga cgcgatcccg
    34621 acagcaatgc ccaacaccac acgagcggcg attaacatct ctacgctggt cgcaaaagcg
    34681 gaccctatcg aaccgagtac aaacaggatg gcccccgcca tcaggctgta tttacgcccc
    34741 aggcggaacg acagccaacc attaaacagc gcaccaattg ctgcaccgag catcatgcta
    34801 ctgaccaccc attcctgcaa acgactggtc agcacaaagt gatcggtaat gaacggcaac
    34861 gctccggcga ttacgccgat atcaagacca aataacaatc ctgcgaccgc agcagctacc
    34921 gaaacaaaca tattcatacg ccgcgtatcc cgcagaggac gtggcgttaa agcagattcc
    34981 gtattgatag taaccatttt ttcctgccag cagagagtaa gacatagtga aaaaatacgt
    35041 gaacaattca cacaggtgtc aggtcggaaa tagcataaat atggattaa
//
LOCUS       DACADF010000034        34913 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.034, whole genome shotgun sequence.
ACCESSION   DACADF010000034 DACADF010000000
VERSION     DACADF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.034"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            72..1727
                     /locus_tag="ISB30_001586"
     CDS             72..1727
                     /locus_tag="ISB30_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HAP1989231.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1818..3053)
                     /locus_tag="ISB30_001587"
     CDS             complement(1818..3053)
                     /locus_tag="ISB30_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAP1989232.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYHEKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCGFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLANETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQMIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPELNYLCAGYQRYFRHLPPYLKAMVDLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(3200..3276)
                     /locus_tag="ISB30_001588"
     tRNA            complement(3200..3276)
                     /locus_tag="ISB30_001588"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3240..3242),aa:Pro,seq:tgg)
     gene            complement(3319..3405)
                     /locus_tag="ISB30_001589"
     tRNA            complement(3319..3405)
                     /locus_tag="ISB30_001589"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3369..3371),aa:Leu,seq:cag)
     gene            complement(3426..3501)
                     /locus_tag="ISB30_001590"
     tRNA            complement(3426..3501)
                     /locus_tag="ISB30_001590"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3466..3468),aa:His,seq:gtg)
     gene            complement(3560..3636)
                     /locus_tag="ISB30_001591"
     tRNA            complement(3560..3636)
                     /locus_tag="ISB30_001591"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3600..3602),aa:Arg,seq:ccg)
     gene            complement(3739..5124)
                     /locus_tag="ISB30_001592"
     CDS             complement(3739..5124)
                     /locus_tag="ISB30_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP1989233.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLTVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5315..6055)
                     /gene="rffM"
                     /locus_tag="ISB30_001593"
     CDS             complement(5315..6055)
                     /gene="rffM"
                     /locus_tag="ISB30_001593"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAP1989234.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6058..7410)
                     /gene="wzyE"
                     /locus_tag="ISB30_001594"
     CDS             complement(6058..7410)
                     /gene="wzyE"
                     /locus_tag="ISB30_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAP1989235.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGAAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7407..8486)
                     /gene="rffT"
                     /locus_tag="ISB30_001595"
                     /gene_synonym="wecF"
     CDS             complement(7407..8486)
                     /gene="rffT"
                     /locus_tag="ISB30_001595"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAP1989236.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNMMANDRQREGKMTILVGNSGDRSNEHIAALRAVYQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQTLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLVSVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8483..9733)
                     /gene="wzxE"
                     /locus_tag="ISB30_001596"
     CDS             complement(8483..9733)
                     /gene="wzxE"
                     /locus_tag="ISB30_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAP1989237.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9735..10865)
                     /gene="rffA"
                     /locus_tag="ISB30_001597"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9735..10865)
                     /gene="rffA"
                     /locus_tag="ISB30_001597"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HAP1989238.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNINETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKNGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10870..11544)
                     /gene="rffC"
                     /locus_tag="ISB30_001598"
                     /gene_synonym="wecD"
     CDS             complement(10870..11544)
                     /gene="rffC"
                     /locus_tag="ISB30_001598"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAP1989239.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPDAALLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLIEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11522..12403)
                     /gene="rfbA"
                     /locus_tag="ISB30_001599"
     CDS             complement(11522..12403)
                     /gene="rfbA"
                     /locus_tag="ISB30_001599"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP1989240.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12422..13489)
                     /gene="rffG"
                     /locus_tag="ISB30_001600"
     CDS             complement(12422..13489)
                     /gene="rffG"
                     /locus_tag="ISB30_001600"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP1989241.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDTLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13489..14751)
                     /gene="wecC"
                     /locus_tag="ISB30_001601"
     CDS             complement(13489..14751)
                     /gene="wecC"
                     /locus_tag="ISB30_001601"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAP1989242.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRANELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14748..15878)
                     /gene="wecB"
                     /locus_tag="ISB30_001602"
     CDS             complement(14748..15878)
                     /gene="wecB"
                     /locus_tag="ISB30_001602"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAP1989243.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(15934..16980)
                     /gene="wzzE"
                     /locus_tag="ISB30_001603"
     CDS             complement(15934..16980)
                     /gene="wzzE"
                     /locus_tag="ISB30_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAP1989244.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(16992..18095)
                     /gene="wecA"
                     /locus_tag="ISB30_001604"
     CDS             complement(16992..18095)
                     /gene="wecA"
                     /locus_tag="ISB30_001604"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAP1989245.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18335..19594)
                     /gene="rho"
                     /locus_tag="ISB30_001605"
     CDS             complement(18335..19594)
                     /gene="rho"
                     /locus_tag="ISB30_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP1989246.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19679..19780)
                     /gene="rhoL"
                     /locus_tag="ISB30_001606"
     CDS             complement(19679..19780)
                     /gene="rhoL"
                     /locus_tag="ISB30_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAP1989247.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(19921..20250)
                     /gene="trxA"
                     /locus_tag="ISB30_001607"
     CDS             complement(19921..20250)
                     /gene="trxA"
                     /locus_tag="ISB30_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAP1989248.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20381..21646
                     /gene="rhlB"
                     /locus_tag="ISB30_001608"
     CDS             20381..21646
                     /gene="rhlB"
                     /locus_tag="ISB30_001608"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAP1989249.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21782..23266
                     /gene="gppA"
                     /locus_tag="ISB30_001609"
     CDS             21782..23266
                     /gene="gppA"
                     /locus_tag="ISB30_001609"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAP1989250.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPPQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFEQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFANRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23313..25334)
                     /gene="rep"
                     /locus_tag="ISB30_001610"
     CDS             complement(23313..25334)
                     /gene="rep"
                     /locus_tag="ISB30_001610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAP1989251.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25421..25702
                     /gene="ppiC"
                     /locus_tag="ISB30_001611"
     CDS             25421..25702
                     /gene="ppiC"
                     /locus_tag="ISB30_001611"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAP1989252.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25748..27223)
                     /gene="ilvC"
                     /locus_tag="ISB30_001612"
     CDS             complement(25748..27223)
                     /gene="ilvC"
                     /locus_tag="ISB30_001612"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAP1989253.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRSVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27373..28263
                     /gene="ilvY"
                     /locus_tag="ISB30_001613"
     CDS             27373..28263
                     /gene="ilvY"
                     /locus_tag="ISB30_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAP1989254.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSADKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28260..29804)
                     /gene="ilvA"
                     /locus_tag="ISB30_001614"
     CDS             complement(28260..29804)
                     /gene="ilvA"
                     /locus_tag="ISB30_001614"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAP1989255.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29807..31657)
                     /gene="ilvD"
                     /locus_tag="ISB30_001615"
     CDS             complement(29807..31657)
                     /gene="ilvD"
                     /locus_tag="ISB30_001615"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAP1989256.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQDDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDIIVTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGNKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31722..32651)
                     /gene="ilvE"
                     /locus_tag="ISB30_001616"
     CDS             complement(31722..32651)
                     /gene="ilvE"
                     /locus_tag="ISB30_001616"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAP1989257.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32671..32934)
                     /gene="ilvM"
                     /locus_tag="ISB30_001617"
     CDS             complement(32671..32934)
                     /gene="ilvM"
                     /locus_tag="ISB30_001617"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAP1989258.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32931..34577)
                     /locus_tag="ISB30_001618"
     CDS             complement(32931..34577)
                     /locus_tag="ISB30_001618"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAP1989259.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITHKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34580..34630)
                     /locus_tag="ISB30_001619"
     CDS             complement(34580..34630)
                     /locus_tag="ISB30_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989260.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34717..34815)
                     /gene="ilvL"
                     /locus_tag="ISB30_001620"
     CDS             complement(34717..34815)
                     /gene="ilvL"
                     /locus_tag="ISB30_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAP1989261.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8442 a         9648 c         8853 g         7970 t
ORIGIN      
        1 gggagaggga tctcaattat caatgcttaa ttacgtcatc attttgacga catgaaaccg
       61 gagcattttg catggaattt tcgttttcac ccaaacttct tgttgttgct gtcgccgcag
      121 ctcttcctct catagccagc gcagcagata ccccgtcaac cgccaccgca cgcaaaggct
      181 ttgccggata cgatcaccca aaccagtatc tggttaaacc ggcgaccact attgccgaca
      241 acatgatgcc ggtgatgcag catcctgcac aagacaaaga aacccagcag aaactggcgg
      301 aacttgagaa aaaaaccggt aagaaaccga acgtggttgt tttcttgctg gatgatgtgg
      361 gctggatgga cgttggcttt aacggcggcg gcgtggcggt gggtaaccct acaccagata
      421 tcgacgccgt tgccagccag gggctgattt taacttcggc gtattctcaa ccgagttctt
      481 ccccaacccg cgccacgatt ctcaccggac aatactccat ccaccacggc attctgatgc
      541 cgccaatgta cgggcaaccg ggcggtctgc aagggttaac cacgctgcca cagttgctgc
      601 acgatcaggg ctacgtcacc caggctatcg ggaaatggca tatgggggaa aacaaagagt
      661 cgcagccgca gaacgttggc tttgatgatt tccgcggctt taactcggta tctgatatgt
      721 ataccgaatg gcgtgatgtt catgtcaacc cggaagtcgc cctgagcccg gatcgttctg
      781 aatacatcca aaaactgccg tttagcaaag atgacgttca tgccgtgcgc ggcggtgaac
      841 aggaagccat tgccgacatt acgccgaaat atatggaaga tctggatcaa cgctggatgg
      901 aatatggcgt taagttcctc gacaagatgg cgaagagtga taagcctttc ttcctctatt
      961 acggcactcg cggctgtcac ttcgataact acccgaacgc caaatatgcg ggtagctctc
     1021 cggcgcgcac ctcttacggc gactgcatgg tggagatgaa cgatatcttc gccaatctgt
     1081 ataaagcact ggagaaaaac ggtcagcttg ataacacgct gattgtgttc acctctgata
     1141 acggtccgga agccgaagtt ccgccgcacg gtcgcacgcc gttccgcggt gcgaaaggtt
     1201 ctacatggga aggcggcgtt cgtgtgccaa ccttcgttta ctggaaaggc atgatccaac
     1261 cgcgtaaatc tgacggcatt gtcgatctgg cggatctctt ccctacggcg ctggatctgg
     1321 cggggcatcc tggggcgaaa gtagcgaatt tagtaccgaa aaccaccttt atcgatggtg
     1381 tggaccagac atctttcttc ctgggaacaa atggtcagtc taaccgtaag gccgagcatt
     1441 acttcctcaa cggtaaactt tctgctgtgc gtatggatga gttcaagtat cacgtcctga
     1501 ttcagcaacc ttacgcttat acccagagcg gatatcaggg tggattcacc ggcacagtaa
     1561 tgcaaacggc gggatcgtcg gtgtttaacc tgtacaccga tccgcaggaa agcgactcta
     1621 tcggcgtgcg ccatatccca atgggtgtac cgctacagac cgaaatgcat gcgtatatgg
     1681 agatcctgaa aaaatatcca ccacgcgcgc agattaaatc tgattaagcc ggcgctatcg
     1741 ctgagatcta cctttgccgg atgcgatgct gacgcatccg acaatcaacc gcaggcggcc
     1801 acaaaatggc tgctttttta cttattcacc accagcaaat gcgcctgcat aatgtcgctg
     1861 gccggacgac cgtgcgccag caaatcaacc attgctttaa gatatggcgg tagatggcgg
     1921 aaataacgct gatacccggc acacaaataa ttcagctcgg gtttgccgct ggcatcgagc
     1981 ataaagcggt gtttcgggca gcctccccag cacgctttta acacgttgca actgcgacac
     2041 tgtgccggta actgcttgaa tttatcttca ccaaatgcct gctgttgcgg ggaatcaatc
     2101 atttctgcaa tcatctgctg aagcatattc cctagtcgat actgcggata aacatagtga
     2161 tcgcaggcat aaacgtcacc gttgtgctca acaatcaccg agcgcccaca ggttggctga
     2221 tgatggcaaa ccgcgcccgg tgcgccgaca aaattggcga acgcccattc gatattcatt
     2281 acgaaaatct tgccgacgtc gcgcttaatc cagtggtcga aaatcgccac cagaaactca
     2341 ccgaactctt gcgggcacac cgaccattcc gtcagttcgc cctgaatatc gccgggagca
     2401 tgtagcttaa gtccagcatg agcagccgtt tcattagcca ggcgctcgac caccggaata
     2461 aactggatga actcaacacc tgcatcacac agaaaatcat agacttgcaa aggttgtaac
     2521 gcactggtgc gattgacgca gaccagcacg ttatagtcga catgatgttt ttgcaggagc
     2581 gtcagggcac gcatcaccag cttatgcgtg ggtctgccac ctttggtcac gcgatattga
     2641 ttgtggatct cagccggacc atccagcgat aaccctacaa gaaaatgatt ttctgccaga
     2701 aatccacacc attcgtcatc gagcagcacg ccgttagtct ggaagctatt acttatcttc
     2761 ctgccagcac catatttcgc ctgtagcgcc acggcacggc ggtaaaactc cagcccgagt
     2821 aacgttggtt cgccgccctg ccaggtaaaa gccacttcgt tttgggtttc gctggcagcg
     2881 atatagtgac ggacatacgc ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg
     2941 tggtaaaggg attgtttttc gagataaaaa cagtagtcac agttcagatt acaatcagaa
     3001 ccgctcggtt tcgccatcac atgaaaagca cgcgttggaa cctgttgctg catggtcgct
     3061 ccttgcctga agtcatcctt caataagtta agggcgaaat cataccgtgg cgcaatgcgt
     3121 tattacgggc gtttattgcg aagggaaagt gacgaaatgc acagaaaaca aaaaaccccg
     3181 ccgaagcggg gttcaaaatt ggtcggcgag agaggattcg aacctccgac ccactggtcc
     3241 caaaccagtt gcgctaccaa gctgcgctac tcgccgaaat actgcttttt gaatttttag
     3301 ttcaattctt taaagtcgtg gtgcgagggg ggggacttga acccccacgt ccgtaaggac
     3361 actaacacct gaagctagcg cgtctaccaa ttccgccacc ttcgcattgt cacaacttct
     3421 aataatgggg tggctaatgg gattcgaacc cacgacaact ggaatcacaa tccagggctc
     3481 taccaactga gctatagcca ccactacaaa tcttgttacg cggtattact accaccgcag
     3541 ctcaagcgcc gggactaaat ggcgcgcccg acaggattcg aacctgagac ctctgcctcc
     3601 ggagggcagc gctctatcca gctgagctac gggcgcttag cgccgttgcg ggggtggata
     3661 atacggactt cccacccctc tgtctagtcc ctttttaaat aaattgcgcg tttggttacg
     3721 gtttgtgcgt tttgctgctt attcctccag tttatgcgct ttaccgtggc gattaagccc
     3781 aaaaacttta taaatcgccg tcaccgtcag cataaagatg atgccgacaa acagcgacat
     3841 acgcgtatct tcattaaagt acatgccgat caaaacgcaa atcaggaatg ccattgttac
     3901 gtagttggcc caagggaaca ggattgagcg gaacggatgg ctggcaattg ccgctttatg
     3961 cgcacgacga aaacgcagct ggctaatcaa tatcacaaac catggcacca tccccggaag
     4021 cacgctggca ctgtagacat agacaaacac acgctgcgga ttgggaatga tgtagttcag
     4081 gcatgagcca attagcagaa tagcaataga taccgccaca cccgcaaccg gtacgccgtg
     4141 acgggaaact ttcgccatcg ccgccggtaa ctgacggttt ttcgccagtg catagagcat
     4201 acgtccgcaa ctgtacatgc cactgttaca gccagaaagc gctgccgtca gcaccacaaa
     4261 gttaataatg cccgccgcag cggtaatgcc gattttggcg aaagtcagta cgaacgggct
     4321 gccgttgctg cctatttcat tccacgggaa gatggtaacg ataacgaaaa tcgcgcctac
     4381 gtagaaaatc aggattcgcc acagcacctt gcctacagcg ctacgcagcg tcacctgcgg
     4441 attcttcgct tcaccggcag taatgccaat cagctccacg ccctggtagg acgccaccac
     4501 aatacacaat gcggtcagga accctttcca gccacccgca aagaaaccgc catgctctgt
     4561 gagattgcta aaaccaatcg actgcccgcc attgccaaag ccaaagaaaa tcacgcccag
     4621 gccaatgaca atcatcacga taatcgtggt gactttgatc atcgcgaacc agaactcgat
     4681 ttcgccgtac aacctcaccg cagccagatt cgccaacgcc accagcgcca ccgcgatcaa
     4741 tgcgggtatc cactgcgcca tctccgggaa ccagaactgg acataaacgc caatggcggt
     4801 gatttctgag atccccaccg ccatccacat aaaccagtaa gaccaggcgg tgagatagcc
     4861 aaagaacggg ctcatataac gatgcgcgta aacggcgaac gaaccggtaa ccggttcgag
     4921 gaacaacatt tcgcccattg aacgcatgat gaaaaagacg aacagcccgg cgatgatata
     4981 ggccaacaat acggatggcc cggcccattt cagggtactg gcggccccca taaacaggcc
     5041 gacgccaatg gtgcccccca gggcgatgag ttcgatatgt cgagcttcca gcccacgctg
     5101 tagctctggt ttgttatctg ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt
     5161 accggttatc gttatgggta catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg
     5221 gcaaataaag cattaaaaat ttgaatgctt tgtgtaataa aaaagcagac aggcgacgga
     5281 gtgaccactt cgtcgcttta caaagagagg aaaatcagag gttgccggta tagtgccagc
     5341 gtaaataacg cagcaaacga agctgacgct taatgcggct cggctgcgaa agcaggcggt
     5401 agagccactc cagccccagc gtttgccaga ttttcggtgc gcgttttacg tgaccggtga
     5461 aaacatcgta ggtcccgcca acgcccatat acagcgcatc tggatgcacc agacggcagt
     5521 cgcgcatgaa gatctcctgc tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc
     5581 tggcatgaat gcgttcaaac agcgcctgac gctgctcggg tttaaaataa ccatcctggc
     5641 tgccaacgat attcacattc cactggttgc gcagtttagc ttcagtttgc gccagcactt
     5701 caggtttacc gcccacaaga aataccggtg tcccttcttt gcctgcgcgc gccatcagct
     5761 cttcccagag atcggcccca gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta
     5821 ctgaacgtac aacgcttatg ccatccgcat atttaaattc ggcagcgtta attagctccc
     5881 ggacctcggc gttatcttca atggtcagca ttttttcggc attaatggca accagcgttc
     5941 cttgcttaag ctgcccgtca gcgaacagat aatcgagggc gtgctgcata tcacgccaac
     6001 caatcaactg taagccacgc agcgtatagg tcggtgccgt ggtgttgtta ttcattatta
     6061 tccttcaacc tgcgtccgga gcgatgattt tgttcgttta tgaatgagtc cggcgctttc
     6121 aaaaagccag tacaacagtt ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa
     6181 gaccacgcgt gagacaaacg aatccagccc ttcacgcgcc agcacgatca tattgaagat
     6241 cgccccgaag cagaaactgt gcaatatcgc agccttatag cgattagtct cgcggttgcc
     6301 cagctcatac agccagtcga accatttgat gatcagtcca acaacaatcg ccccgagcgg
     6361 gatgaacaac gcgccgccca tcaccaccag tgagcctata agcgtaggcg agatcgccag
     6421 tccggagtgg ttattcagca cttcccaggt aaagtagttg gcggagttca gcaccatact
     6481 cgggcgaccc ggccacagcc aggaagggat aaagacatag aaatcgcgga caattggagc
     6541 caggccctgg aagtcgatgt tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg
     6601 tgaaaaggtg tcgcgagtga gatagagaaa cgtatagaac gcttcatcgc cgctcacatt
     6661 cattccatag cgttttaacg ccagccagaa catgcccaca atccccagca cgcccgccgc
     6721 cgccagcatc cacaacgaaa tccagccgcg aataatgcca ataaacagga agatagcgaa
     6781 tgcgatgatg atattggcgc gagtgccgcc gacaatcata taagtcagca agccaaaagc
     6841 gaccgtgctg acgaggaaaa acagccacgc tttgctgtcc tggcgcagaa agtagaccac
     6901 cagcatcgcc gggatgaaaa agtaaaagaa gcgttttaac gccacgccgg agacttcact
     6961 ggagaagatc tgactgtagg agttaagccg gaacagcaaa aagccgttgt gcatgaagaa
     7021 gatgccgacg cttaccagcg cgatgcccat caggatcacc cacgtgagat tggtttccac
     7081 gcggttcatg gtaaacagcg gacggcgcgg tacatcagca acgcgtttgc gtaggcgggt
     7141 tttgtaggtg acgtagtaaa ccgcgtagaa gcagcccgca gaaagcaacg cctgcaacaa
     7201 gatttctggc ggcgcggcac caacatcaaa gcgaaaaacc agcacgctgg tcagcgggaa
     7261 gccgaagaaa aaggtgagca aaaacagcaa tgaaaagaag acattgaagt taaagcgcac
     7321 acggcgaaac tcaaaccagg tcagcgtggc aataaacagc gtgcagagca gccagacaac
     7381 aaacaggcca ctgaattgca gcagactcat gcgacctccc cggcggcaat cgccaacgcc
     7441 cgctgccagc cttgcagata gttcgggcta aagaaggcaa tggtgttttt atccaccgac
     7501 accaactggc gctgcgcttc acgcacaata tcctcgttga gatcgtcggt agtaaacagc
     7561 accggcaaat gctgttccgt catatcctgc cagaacggat tttcccggtt aagcacacaa
     7621 ggaatgcccg cctgaatcag taagcacagc gtaccaatgc cctgctggcg ggcaaaaata
     7681 aagtaaccaa gatcgcactg acgaagtagc gccagatagg cgtcaaattc cagtttttcg
     7741 ctcagagttt gtagattttc ttcgctgaat aactccagcc ccgcctgacg aacttcctcg
     7801 atgtacgctt cgttattagg cggatatccc atcggcacca ccacttttac cgtatcgcca
     7861 aattgctgat aaacggcgcg caaggcagca atatgttcat tgctgcggtc accggagttc
     7921 cccaccagaa tggtcatttt cccttcacgt tgccgatcat tcgccatcat attgagtgaa
     7981 gggtccatcc gcgtcgggaa gtacagcagt tctccccgca cctttggatg cgttttggca
     8041 aaaaagctca aatcaccgcg ggtggcaaat acacagccga ctctcttttg cgccaggcga
     8101 cgtagcgggt aaaaaagctt atatctcaag ccactggaaa gctcgtacag gtctgccccc
     8161 cagatatgcc agtaaaactg gctgggctta atgccaccac tcagcagagc cagccacagt
     8221 gtgggattga actgaccgtg gaagaaaaaa cgctgctgac ggttagcttt tgctttcgcg
     8281 atgaccgctt ccgccagcga ttttttccca gggaaaaatt gcacagaaag cgccggacag
     8341 ctatcactta agccgtcgtc cttgccaaca accataaact cgcgcgcgtg ctcgctcgtc
     8401 gcggccagcg cgtcattgaa aaaccgcaaa acggttcggt tatggtgagg gatatccgat
     8461 cccagtacgt gaatcagtac agtcatgccc gcctacgcca gagtaaaaac acgccacaac
     8521 aaagagaaaa atagacgata taggttgcca tataggcctg cgccgcgccc agtgcaccat
     8581 gcgcagggat tagccagtgg gcaaatacca tcaataaagt gaactggctg acttccgcca
     8641 gaatataaaa ccgcagtgac gctttggcga tcaccagata accaaagaca taagcgccca
     8701 cttttaacac atcacccacc aactgccagg caaagagatc gcgcatagcg gtaaatttat
     8761 tcgacaacag cagccagata gcaaaatcac gcagcaacca gacggtaaaa ctcgccgccg
     8821 ccactgccgg caagacgaat ttaagcgatt taaccacttc ccgggtgata tcgcgctttt
     8881 ccgttagccg cgacaacgtg ggcagcaaat atacgctgaa cgatgccgta ataaattgca
     8941 ggtaggcatc ggaaatactg ctcacccctt gccagatccc cacctcatcc cagctatact
     9001 gcgccgccag cagtttacgc atcatgatgt aagcaacagg caaggtcacc gacgtaatca
     9061 acgccatgag cgtaaatttg ctcaactgcc ctgccagacc gttatcccag ctgggtttca
     9121 gatagcttaa cgggatgaca ccacgtttga tcaacatgat ggcggcagga attaccacca
     9181 gcgcgggaat cagcgccaga cccagcaacg ccccttcata accgcccaaa cggtaactga
     9241 cgtagtacgc gagaacgcca atcaagctgc caacaatcag agataacgca ttacctgcgg
     9301 catcgcgaaa gcctttcatc agcgccagta acaggttgcc ccaggcgatc cccatttgca
     9361 ccagcgccac taaacgcacc agcccctgat agtcggtatt accaaacaat ccctggctga
     9421 ttggcgcagc tgccagcaca aaaaccagcg ccatcagcgt agagaaacca agtaccatcg
     9481 ctgatgaagt gccgaccacg cggcgcagct gttgcggatt atcatggtac tgggcaacgt
     9541 atttggttac accgttaaag atgccagccc cggcaagcac gccgagcacg gtaatcaact
     9601 ggcggaaatt tgccgccagc ccaagccccg ccggaccaaa tgacaccgcc agcaacttac
     9661 cgactagtaa cccggcacca atcttgacca gtgtactggc cgccgtccac aaggacgctt
     9721 ttgccaacga catatcagga gaagtagttc aacaaagtcg caattaccgt acgctgattg
     9781 acgggcgaca ggttgtagaa cagcggcagg cgcagcaggc gctcgctctc tttggtggtg
     9841 tagcgatctt caccgtggaa ctcaccaaag tgttcccccg cagggcaacc gtgcagcgga
     9901 atgtaatgaa acaccgccat gatttccgct tctttcagaa agttaatcaa cgcgctccgg
     9961 tcatcaatat cccgcagttt aatgtagaac atatgcgcgt tctgcacgca gccatcggga
    10021 atcgacggca gctcgatacg cccatttttt gccagaggcg ccagtgcatc gtagtagttt
    10081 tgccacagcg ccagacgctg ctgattgata cgatccgctg cttccagttg cgcccacagg
    10141 tacgctgctt gcagatcgga catcaaatag ctggagccaa tatcgcgcca ggtatattta
    10201 tcgacctgac cacggaagaa ctggctgcgg tttgtgcctt tttcacggat gatctcggct
    10261 cgttcgatca acgctttatc gttaatcagc gtcgcgccgc cttcaccacc cgccgtgtag
    10321 tttttggttt catggaagct aaagcagcca atatgaccaa tggttcccag cgcacgccct
    10381 ttgtaagtgg acatcacgcc ctgagcggca tcttccacca caaatagatt atgcttcttc
    10441 gccaacgcca taatagtgtc catttcgcag gccacacccg cgtaatggac cggcacgata
    10501 acgcgcgttt tgtcggtgat cgccgcttca atcagcgttt cgttgatgtt catggtgtcc
    10561 gggcgaacat ccacaaagac gattttcgcg ccacgcagca caaaggcatt ggcggtggag
    10621 acaaaggtgt agctcggcat gatcacttca tcgccaggct ggatatcgag cagcagcgcc
    10681 gccatctcca gcgaagcggt gcaggacggc gtcagtaaca ctttggcgct gccaaaacgt
    10741 tgctccagcc actgctggca gcgacgggta aaaccaccat cgccgcacag tttgccgcta
    10801 cccattgccg actgcatata gtcgagttcg gttcccacca ccggcggtgc gttaaatgga
    10861 atcatgtgat cacctgtata accagtacgc ggtgctttct acattcgcac cactttgtat
    10921 gtatcgttta agcgcggcgg tgttgcccat ctgggtcgcc acccgcaaag ttgttttacc
    10981 gcgagcatac gcccagttta gcgccgtttg cattagctca gcacctgcac cgcgtccagc
    11041 cagcaggcca attcgcgcat ctgtcgcatt gagttcccgt aaagagacat aaccgcgaat
    11101 atcgccggac gccgcacgta aaatcagaca ttgatgatca aaggtgccgc gcacggcatt
    11161 ttcaatccac tgtgcataaa agcgaccgct ggcgtcaggc gcataccacg gcgcacgaaa
    11221 acggctttgc gcaaatgcgg cgctggctaa ctgacgtaat gcgggaatat cggtctcttg
    11281 tgccactact gcaccgctat cactgacatt gttcacgggt agtgccaaat caacttcacc
    11341 ttctatcagg gagaatccca gctgttgcag agcatccagt tcacccgtat ttgatgccgc
    11401 aattttggcc tgcacccgtg accacggcgc taaggcgtct ggcgtcagga gtgccgcgtc
    11461 agggctaata cgcacaatgg cgctgttaac accaaagaag gcgttttccc aggttagtgg
    11521 ctcaatactg gcgcggacgg gcacgaagta actccagcag atattggccg tagccagttt
    11581 tcgctaatga actggcagca cgcttcacac cctcgtcatc gagccagccg ttacgccagg
    11641 caatctcttc caggcaggca atcttaaagc cctggcgttt ttctactgtc tgcacaaaag
    11701 tgctggcctc aatcaggctg tcgtgagtgc cggtatccag ccaggcaaat ccgcgcccga
    11761 gcagttcaac ggtcaggttg cccgcctcga ggtacatctg gttgatggag gtaatctcca
    11821 gttcaccacg ctccgacggc ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga
    11881 aataaagccc ggtcaccgcc cagtttgatt tcggctgttt tggtttttct tccagcgaga
    11941 tagcgcggaa attgtcgtca aactccacca cgccaaagcg ttccgggtcc atcacctgat
    12001 agccaaatac cgtcgcccct tccgtgcgcg ccgcaacatg acgcagtttc ggactgaagc
    12061 cctgtccgaa gaagatgtta tcgcccagca ccagacaaga aggttcaccg ttgaggaagg
    12121 tttcaccgat gataaaggcc tgcgccaggc cgtccggact gggctgttcg gcatattcca
    12181 gctggatacc gaactcactg ccgtcaccca gcaggcgctg gaaataacct ttatcttccg
    12241 gcgtagtgat gatgagaatt tcgcggatac cggccagcat cagcaccgac agcggatagt
    12301 aaatcattgg cttatcgtaa atcggcaaca gttgcttaga tacgccgcgc gtaatcggat
    12361 gcaatcgggt gccggaaccg cccgccagga taataccttt catgtttgcc tccgctgaaa
    12421 attagccttt cagacctaaa cgctcgccct gatagctgcc gtcctgcacc tgcttccacc
    12481 agctttcatt agccagatac cactgcaccg ttttacgcat tccactttca aaggtttcct
    12541 gcggcagcca gccaagttca cgggcaattt tcgaagcatc aatggcataa cgcagatcat
    12601 gccccggacg gtcagcgaca aaggtgatca ggtcacgata gtgcgccacg ccgtgcggct
    12661 tattcggagc cagttcttcc agcagctcgc aaatggtttc cacaacatcg agattcttac
    12721 gctcgttgtg accaccaata ttataggttt caccgacttt cccggtggtc gccacgcaat
    12781 acagcgcgcg ggcgtgatct tcaacataca gccagtcacg gatttgctgc ccgttgccat
    12841 ataccggcag cggtttaccc gccagcgcgt tgaggatcat cagcgggatc agtttttccg
    12901 gaaagtggta aggaccgtag ttattcgagc agttggtgat aagcgtcggc agaccatagg
    12961 tccgcagcca ggcgcgcacc agatggtcgc tgctggcttt tgacgcggaa taagggctgc
    13021 tcggcgcata cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg tcaccatata
    13081 cttcgtcggt ggagatatga tgaaaacgga acgctgattt tttatcttcc gtcagcgcag
    13141 tccagtaagc ccgcgccgct tcaagcagtg tataagtccc gacaatgttg gtttcaataa
    13201 acgctgccgg gccgtcaata gaacggtcaa catggctttc ggctgccaga tgcatgacac
    13261 agtctggctg atactcagtg aatacgcgcg ccagttctgc ccgatcgcag atatcaactt
    13321 tctcaaaggc aaagcgctcg ctttgcgcga ccggtgccag cgacatcagg tttccggcgt
    13381 aggtcagcgt atcgaccacc accaccgcgt cgctcgtttc gttgatgata taacgcacca
    13441 gcgccgagcc aataaacccg gcaccacctg ttatcagaat ctttctcatc agcgccagac
    13501 tcctttggca tcgacgacat actgctgatg gacgttgtcg ccattgataa ctttgaactg
    13561 actatgatcg accagcatca ccagcacatc tgccgttgcc agcgcctcgt caagctgcgc
    13621 cagagtacaa agcccggtca gtttcttcgg caactggtgg atgttaggct caacaaccag
    13681 cgtttcgccg ctgtgccact gggcgatcag ttcagcgatt tccattgccg gactttcgcg
    13741 caggtcatca atattcggtt taaacgccag accaaagcag gcgattttca gttcattggc
    13801 gcgtttatcg gtagcagcca gacaatcagc caccgccgct ttcacctgat cgataaccca
    13861 gaacggtttg tgatcgttca cttcgcgcgc ggtacggata agccgcgcct gctgggggtt
    13921 ctgtgccacg ataaaccacg gatcaacagc gatgcagtga ccgcccacgc cagggcctgg
    13981 ctgaagaata ttgacgcgag ggtgacgatt cgccaggcga atcagttccc agacgttaat
    14041 cccctgatcg gcacaaatca gcgacaattc attagcaaaa gcgatattca catcgcggaa
    14101 gctgttttcg gtgagcttac acatttccgc cgtccgcgag ttagtgacga cacactcacc
    14161 ttcgaggaaa attttgtaca gttcgctggc gcgggccgaa caaaccggcg tcataccacc
    14221 aatcacgcga tcgtttttaa tcagctcgac cataacctgg cccggtaata cgcgttccgg
    14281 gcagtaagca atgttgacgt ccgccaactc gcccacctgc tgtgggaaag tgagatctgg
    14341 acgcatctct gctaaccatt ctgccatctt ctcggttgac cccaccggcg aggtggattc
    14401 aaggatcact aacgcgcctt ttttcagcac tggcgcaatg gagcgagcag ccgattcaac
    14461 gtaggtcata tctggctcat gatcgccttt aaacggtgtg ggtacagcaa tcaaccaggc
    14521 atccgcttca actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg cagtttttac
    14581 tacgctcgcc aaatcaggct cgacgatatg gatttcgcca cgattgatgg tatcaaccgc
    14641 gtgttggttg atatcgacac caatcacctg tttttgcctt gaggcaaacg cagctgctgt
    14701 aggcagcccg atgtaaccca gtccgataac agaaatggtc gcaaaactca tagtgatatc
    14761 cgattatttt ttaacgcttc cagaatgcga gagcatgcct gaccatcacc atacgggtta
    14821 tgggcgcggc tcatagcttg atattcgttt tcgtctttta aaagacgcgt cacttcctcg
    14881 acaattcgct gcttatccgt gccgaccaga cgcaccgtac ccgccgtcac cgcttccgga
    14941 cgctcagtgg tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc ttcttcctga
    15001 atgccgcctg agtcggtcaa aatcagccag gcgtggttca tcagccagac aaacggtaaa
    15061 tactcctggg gatcgatcag aatgacattt ttcacatgcc ccagaatgcg attgaccggc
    15121 tctctgacgt tcgggttgag atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg
    15181 gcgatgtctg ccagcgcatg gcagatttct tcaaagccac gaccgaagct ctcacgcctg
    15241 tgaccggtca ccagaatcat ctttttatcg gggtcgataa acgggtaatt tgccgccagt
    15301 tctgaacgca gcgtatcgct gctcatcacc tggtcacgca cccataacag tgcatcaatg
    15361 actgtattac cagtaatgaa gattcggcta tccgcaacgt tttcacgcag caagttttgc
    15421 cgggaagttt cggttggaga aaaatgatac atcgccagat gcccggtcaa tgtacggtta
    15481 gcctcttccg gccacggcga atagagatcg cccgtgcgca gaccagcctc aacgtgacca
    15541 acaggaatac gctgataaaa cgccgccagg ctggttgcca gcgtcgtcgt agtatcgccg
    15601 tgaaccagca cgacatctgg tttgaactcg gcaagaatag gttttagccc ttccagaatc
    15661 cgacaggtta tctctgtaag gccttgtcct ggctgcatta tgttgagatc gtagtcaggt
    15721 acaatggaaa agagtttcag cacctgatcg agcatctccc gatgctgcgc agtgacgcaa
    15781 actttagcct caaaaaaagg atcttttgcc aacgcatgca ccaacggtgc catcttgatg
    15841 gcttccgggc gcgtaccaaa tacagtcagt actttcacat cgattctctt cgaataagcg
    15901 gcgagcgcct ttgcgctcac cgcagcagtg ttgctatttc gagcaacggc gggttaatgc
    15961 gacaccagcc ccgatcagtc ccccgacaat gccccacata atcatcagga aggcacgacg
    16021 tgggctatcg cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg
    16081 cggatccagg gttggaccaa cattcagggt gtttaacatg gcccgatttt gatcatagtc
    16141 gagatcaaag gccggaccga cggcctgtaa attttccagt cgagcctgga gcattggacg
    16201 cccaagcagg aacatttctg aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg
    16261 cgaaatatta tgctgctcag caattttcag cgcctgttca atactgttca tccggcggtc
    16321 atagatggct ttcgccacct cttcctgacg cttcacctga gctttcatct ggatagtgcg
    16381 tgccgcccat gcgcctttca gctcatcatt cagatggctg gctgcacgct ggctggcaaa
    16441 agcaacatac tgacgtaaca ggttattagc gtcaggcgcg gtttcggcaa taagcttcac
    16501 gctgtcattg accgcgcggg taaagtcacc gggaataaac acgatgttgt taatcatttc
    16561 atccagcaac gccgcatcgg ctttgctgtt gcccaccatc cgctgtttgt aatagtcggt
    16621 ttgcagccag aactctctgc gggtatccca cgaggccagt tgcataacaa actctttata
    16681 agcttcgtcc atgaccgatg gttggtcggc agaagccatg tttgaacgga catccaggtt
    16741 acgcaaaaat tgctgctgtg agtagtaccc gcccagcata ttcaccgttg gacgatcggt
    16801 aatcgccgtc gcgctccact cctgacgagc aaaaaaagta tacgccagcg cgattaacgc
    16861 aaacgccagc cccatgccaa taatccatag cttcccagcc cacaaggtac gaaacaaccc
    16921 acgaatatcc agttcatttt cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat
    16981 cacatcctca tttatttggt taaattgggg ctgccaccac gatttctacg cagtctgcgt
    17041 tttacgcgct taataaagcg agcaactttc caggcacgct tgatgcaata tccatagagg
    17101 aagaatgcta gcaaaaagag caccagcatg acccactccg ggacaaaatg agaatattct
    17161 gccagcacgc caatggaagc gagcaatgct gcggcaaggg taatcagcac aaacgcctgg
    17221 cgggaagtaa acccggcacg catgatcaaa tggtgaatat gctgacggtc tggagagaat
    17281 gggctcatgc ctttacgcag gcgacggtac ataatcgcca ccatatccat tagcggaatg
    17341 gcgataatcc acaaagcggt aaccgggctg atgggatgag ttttgccctg ggtcgtttcg
    17401 agcaggatcc agataacggt aaaaccaatc agcgtactgc ccgcatcccc cataaagact
    17461 ttgtagcggc gacccaggat accaaggtta agcatgatgt atggcaggat ggcggcgatc
    17521 atcgcaaagc accagattgc gagactggtt tgcccgtcga accacaaaat cataccgatt
    17581 gctgcaaacg agacgcagga caacccgccc agcaagccat caatgccatc aaccatgttg
    17641 aacgcattaa tggccgccca gacggcaaat agcgtcagga agtaaccaaa cggtccgagc
    17701 accatctccc aggagccaaa gatataaccc aggctactga gataaagttt gccgaacacc
    17761 atcataacaa tgccaacagc ggcctgtatg gtggctcgga tttttacgct gatatcaaaa
    17821 cggtcatcca gcgcgccaat gaaaacaagc acaccagcac aagcgagata gagagatgca
    17881 tgcggaatat agtagtcgac aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc
    17941 cccccaacga gaggtatcaa tccctggtga cgtttgcgga agtttggttt atccactaaa
    18001 ccgacttttt ttgccacctt acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata
    18061 ctgatgagat cagtactcac tgtcagtaaa ttcacaatgc atgctctcag agaaaattat
    18121 tagcagaagt ataaccacga agacctttat tcagaaagga aagtccgttc cgaacctacc
    18181 agcactatgc gaaaattaac cacttgatat gcaaattgca taacacacca gcaaattcca
    18241 ggaattttcc taacatcgcc agcgcggcat taagattaca gatataaaag caaaacgcca
    18301 cgtaaacacg tggcgttttt ggcataagac aaatttatga gcgtttcatc atttcgaaga
    18361 aatcgtcatt ggtcttggtc attgccagtt tattaatgag gaattccatt gcatcgattt
    18421 cgcccatcgg gtgaatgatt ttgcgcagga tccacatttt ctgcagttct tcctgagtcg
    18481 tgagcagctc ttctttacgg gtaccggaac ggttgtagtc gatagccggg aagacgcgtt
    18541 tttcagcgat cttacgagag aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt
    18601 agataacttc gtccatttta gaaccggtat cgataagcgc cgtcgcgata atggtcaggc
    18661 tgccgccctc ttccacgtta cgcgccgcac cgaagaagcg tttcggacga tgcagggcgt
    18721 tggcgtccac accaccggtc aacactttac ctgacgccgg aacaacggtg ttgtaagcgc
    18781 gcgccagacg agtgatggag tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc
    18841 gtttcgcctt ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa
    18901 aggtagaagc aacaacttca ccttttacca gtcgctgcat ctcggttact tcttccggac
    18961 gttcgtcgat cagcagaacc atcagcacac aatccgggtg gttgtaagca atgctctgag
    19021 caatgttctg cagcagcatg gttttaccgg ctttcggcgg tgccacaatc agaccacgct
    19081 gaccacgacc gataggtgat gccagatcca gtacgcgagc ggttaaatct tccgtagaac
    19141 cgttaccacg ttccatacgc agacgagagt ttgcgtgcag cggggttaag ttctcaaaga
    19201 ggattttgtt gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg
    19261 caaaatagcg ttcaccttct ttcggcgggc gaatcttacc agagatggta tcaccagtgc
    19321 ggaggttgaa acggcggatt tggctagggg aaacgtagat gtcatcagga ccggcgaggt
    19381 aggagctgtc tgcggaacgg aggaaaccaa atccatcctg caatatctcc agtacgccat
    19441 caccaaagat atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct
    19501 gcttacgcat acgagccagg ttttccagcc ccatattttc gccgagagtg atcagctcag
    19561 aaaccggcgt attctttaat tcggtaagat tcatagtggt gtgagttctt aaacttgggg
    19621 taaatctcga acttaatgtt gtgaatggta tggcaggatc atccatgcct gtttacggtc
    19681 atcgactcat gtcttttcgc tgcctggtca caggagagta cgcagaactg aaacgacaag
    19741 acggattgag tgacgagcca gaaatctgtt cacttcgcat ttaagataaa acgggaagcg
    19801 ttggaaatta caagattcaa acttaataag gtatgtttaa tacgaagtca acactaagtt
    19861 agcatgactc acgccgggcg tccagttttt agcgacgggg cacccgaaca tgaaattccc
    19921 ttacgccagg ttagcgtcga ggaactcttt caactgacct ttagacagtg cgcccacttt
    19981 ggttgccgcc acttcaccgt ttttgaacag cagcagagtc gggataccac ggatgccata
    20041 tttcggcgca gtgccagggt tttgatcgat gttcagtttt gcaacggtca gtttgccctg
    20101 atattcgtca gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc
    20161 tgcccagaaa tcgacgagga tcgccccgtc cgctttgagt acatccgtgt caaaactgtc
    20221 gtcagtcagg tgaataattt tatcgctcat atataactcc acaggaataa gcctggcgtg
    20281 ttggtttcgt tgttggtgta acattaacca actaaaggtt gactttattt caccggatac
    20341 gctttcgtaa agcaatagta agctgatatt ctaccacact atgagcaaaa cacatttaac
    20401 agaacagaag ttttccgact tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa
    20461 agggtttcat aactgtacgc ccattcaggc actggccctt ccgctgacgc tggcgggtcg
    20521 tgacgtagcc gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac
    20581 gtttcattat cttctctctc atcccgcgat tgctgatcgc aaggtgaatc agccgcgtgc
    20641 cttaattatg gcaccgacgc gtgaacttgc cgtgcagatc cacgccgacg cggaaccgct
    20701 ggcggaagct accggcctga agctgggtct ggcttacggt ggtgatggct acgacaaaca
    20761 gctgaaagtg ctggaaagcg gcgttgacat tctgattggc accacgggtc gtttaattga
    20821 ctacgccaag cagaaccaca ttaacctcgg tgccattcag gtggtggtgc tggacgaagc
    20881 cgatcgcatg tacgatctgg gctttattaa agatatccgc tggctgttcc gccgtatgcc
    20941 gcctgcaaac cagcgcctca acatgctgtt ctccgccacg ctttcgtacc gggtacgtga
    21001 actggcgttc gagcagatga acaatgccga atatattgaa gtggaaccgg aacagaaaac
    21061 gggccaccgt attaaagaag agcttttcta cccttctaac gaagaaaaaa tgcgtttgct
    21121 gcaaacgctg atcgaagaag agtggccaga cagagcgatt attttcgcca acaccaaaca
    21181 ccgttgtgaa gagatctggg gccacctggc ggcagatggt catcgtgtcg gtttattgac
    21241 aggcgatgtc gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc gtggcgatct
    21301 ggatattctg gttgccaccg acgttgccgc gcgtggtttg catattcctg ctgtgacaca
    21361 cgtctttaac tacgatttac ccgatgactg tgaagattac gttcaccgta ttggtcgtac
    21421 aggtcgcgct ggcgcaagcg gtcattccat cagcctggcg tgtgaagagt atgcattgaa
    21481 tttgcctgct attgagacct atattggtca ctcaattccg gtaagtaaat acaatccgga
    21541 cgcattgatg accgatctgc caaaaccgct gcgcctcacg cgcccgcgca caggcaatgg
    21601 tccgcgtcgt actggcgctc cgcgtaatcg tcgtcgttca ggttaaagaa aaaatgtcat
    21661 actccaaatc attcaagctg tatcaagatg gcaagagaat gaatcccgat gagtttactg
    21721 tcgtaagtga ttcaggtgaa agaatgcagc caacacagag acagattgaa ggatgaagag
    21781 tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata gttttcatat
    21841 gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaataa aacgcaaagt
    21901 gcgtctggct gctggcctga atagcgaaaa tgccctgtct aatgaagcaa tggagcgcgg
    21961 ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctcccc cgcaaattcg
    22021 cgttgtcgct acggcgacat tacgccttgc cgtcaatgcg ggtgatttta ttgccaaagc
    22081 gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag cgcgtctgat
    22141 ttatcagggc gttgctcaca ccactggcgg tgccgatcag cgcctggtgg tggatatcgg
    22201 cggagccagt actgaactgg taaccggcac gggtgcacaa accacctctt tgttcagcct
    22261 gtcgatgggc tgcgttacct ggctggaacg ctattttgcc gatcgtaatc tggggcagga
    22321 aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg ccgatgaatt
    22381 acggtatcac ggctggaaag tgtgcgttgg cgcttccggc accgtgcagg cgttacagga
    22441 aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc agcaactgaa
    22501 acaacgcgcc attcattgcg gtcggctgga agagctagag attgacgggc tgacgctgga
    22561 acgtgcgtta gtgttcccga gtggtctggc gatcctgata gccattttta ccgaactgaa
    22621 tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct acggtatgtt
    22681 gcatcttacc gtcgagcagg atattcgcag ccgtacgctg cgtaatattc agcgccgctt
    22741 tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact tcttcgaaca
    22801 ggtggaaaat gaatggcatc ttgaagcaat aagccgcgat ttgctcatca gcgcctgtca
    22861 gcttcatgaa atcggcctga gcgttgactt caaacaagcg ccgcaacacg ctgcttatct
    22921 ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aaaaaactgc tggcgacgct
    22981 actgctcaac cagactaatc cggtcgatct ctcatcgctg catcagcaaa atgccgtacc
    23041 accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt ttgccaaccg
    23101 tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac tgttgacctt
    23161 gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta ttgatcagga
    23221 aagccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag taagtgccgg
    23281 atgcgatgct gacacatttt ttccggcctt gattatttcc ctcgttttgc cgccatcatc
    23341 gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc gctgaccact
    23401 ttgcgctcct gttcccaaat caaatcatcc tgcggcagct ccagcaggaa gcggctcggc
    23461 tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa ggtcaattcc
    23521 ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcaat attgtcttca
    23581 tcgatgctgc tctggtgggg caaaaaccct tcttccatac caaccatgta gacataggga
    23641 aactccagcc ctttcgacgc gtgcagagtc atcagttgca cctgatccag ttcttcctcg
    23701 ctctcgccgc gctccatcat gtcgcgcaga gtaaagcgcg tcaccacctg ggtgagcgtc
    23761 atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa cagttggttg
    23821 acgttcttca tgcgcatttc ggcagctttc gggctgggcg atgtttcgta cagccaggat
    23881 tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg ctccgccaga
    23941 cgctggattt ctgccagcca gtgagtgaag cgggtcaatg cttcataacc acgtccgcta
    24001 agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt gcgcgtcatc
    24061 gcccattcac ctagcttttt cagcgtcgcc gggccaatct ctcgcttcgg cgtgttaacg
    24121 atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata agccagcaag
    24181 tccttgattt caggacgaga gaaaaacgac gtaccgccag atattttgta cgggatgcgg
    24241 ttttgcatca ggaatttttc aaatacccgc gactgatggt tgccgcgata aagaatggcg
    24301 taatctttgt actgcgtttt attgacgaag tgatgggcga tcagctcgcc ggtgacgcgc
    24361 tcggcttcat gttcctcgtt attcgcgctt aaaactttta gctctgtgcc gtaacccagt
    24421 tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat gttcgccgct
    24481 ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt cagcgccgga
    24541 aaatcctggc tcagcagcac cagattctgc ggacgtgcgc cgcgccagga gtagatcgac
    24601 tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag tttcaccagt
    24661 tcatactggc tggtgttggt gtcctgatac tcatccacca gcaggtagcg aattttgttc
    24721 tgccagcgct cgcggacttc ttcattgcgt tgcagcagca acgtcggcaa taaaatcaga
    24781 tcatcgaagt cgagaacgtt acaggctttc aggtgtgcat catacagccc ataacaatgg
    24841 gcaaaaatac ggtcccgctc gccctttgcc tctgccgccg cctgcgccgg tgttttgaga
    24901 tcgttcttcc agttggagat ggtcgaaatt agctgttgca ggagaacttt gtcatcttca
    24961 atcagcccct cggtcaactc tttaagcaaa gcaagctgat cggtatcgtc aaacagcgaa
    25021 aagttagcct tcatccccag cgccgcatac tcgcgtttga tgatatccag ccccagcgtg
    25081 tggaaggtgg aaatcatcag cccacgcgcc tctttgcgcc ccagcgtctg cccgacacgc
    25141 tctttcatct cgcgtgccgc cttattggta aaggtcacag ccgcgatatg tcgcgcctga
    25201 taaccgcaac cgcggatcag atgggcgatt ttattggtga taacacgagt tttaccggaa
    25261 cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg ttgttggccg
    25321 gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat aacagcgtgg
    25381 tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa cagcagcagc
    25441 actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgagc agatcaagaa
    25501 tggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccgtcag gcaaacgcgg
    25561 cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata aagtggtgtt
    25621 ttcttgcccg gtgctggaac caaccggacc gctgcatacc cagttcggtt accacatcat
    25681 taaggtgcta taccgtaatt aacagcaagg ccttctccgg gagaaggcct tgaactctga
    25741 atcgctatta acccgcaaca gcaatgcgtt tcatatctgt catatagccg cgcagtttct
    25801 tacctacctg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc agctgcgcgt
    25861 tatctaccgc accttccgga atagctttac ccaggtcgcc cggttgcagc tctgccataa
    25921 acggtttcag cagtggcaca caagcgtaag agaacagata gttaccgtac tcggcagtat
    25981 cggagataac cacgttcatt tcgtacagac gcttacgggc gatggtgttg gcaatcagcg
    26041 gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccagaa tcaaccatgg
    26101 tttcaaacgc cagttcaacg cctgctttta ccatcgcgat catcagtacg cctttatcga
    26161 agtactcctg ttcgccgatt ttgccttcat actgcggcgc ggtttcgaat gcggttttgc
    26221 cagtctcttc acgccaggtc agcagtttct tatcgtcgtt ggcccagtca gccatcatgc
    26281 cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc ggcgccatga
    26341 tctctttcag ttgctcagag agcgcgtaag cacgcagttt cgccgggtta gaaagacggt
    26401 ccatcatcag ggtgatgccg ccctgtttca gcgcttcggt gatggtttcc caaccgaact
    26461 gaatcagttt ttctgcgtat gccggatcgg tgccttcttc caccagcttg tcgaagcaca
    26521 gcagagagcc agcctgcaac ataccgcaca ggatggtttg ctcgcccatc aggtcagatt
    26581 tcacttccgc aacgaaagag gattccagaa cgcccgcacg gtgaccgccg gttgcagccg
    26641 cccaggcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg tgaacggcaa
    26701 tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg tacttcggtg ccagggcatt
    26761 tcggcgcaac cattacgacg gtgatgtctt tacggatctg ctcacccact tctacgatat
    26821 tgaaaccatg agagtagccc agcgccgcgc cgtctttcat cagcggctga acggaacgca
    26881 ctacgtcaga gtgctgcttg tctggcgtca ggttaaccac cagatccgcc tgcgggatca
    26941 gttcttcgta agtgccaact ttgaagccgt tttcggttgc tttacgccag gatgcgcgct
    27001 tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa tcacgcatgt
    27061 tcaggccctg gttcagaccc tgtgcaccac agccgacgat gactactttt ttaccctgaa
    27121 gatagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg cccagctgtg
    27181 ccagctgctg gcgcagattc agtgtattga agtagttagc catggtgatt cctcgtgatg
    27241 ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat gacaggaaat
    27301 ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac gtcaacatta
    27361 agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc ggaaagccgc
    27421 cattttggcc gcagcgcgcg ggcaatgcac gttagcccgt ccacgctctc tcggcagatt
    27481 cagcgcctgg aagacgatct cggtcagccg ctgtttgtgc gcgataaccg cacggtaacg
    27541 ctaaccgaag caggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt gcagtatcaa
    27601 cagctgcgcc acaccatcga tcagcaagga ccgtcgctct ctggcgaatt acatatcttc
    27661 tgctcggtga ccgctgccta cagccatctg ccgccgattc tcgatcgctt ccgcgcggaa
    27721 cacccgtcgg tggagattaa actcacgact ggtgatgcgg cagatgcgat ggaaaaggtc
    27781 gtcactggcg aagcggatct ggcgattgcc gggaaaccgg agactctgcc cggcgcagtg
    27841 gcgttttcga tgctggagaa tctggcagta gtgctgattg cccccgcgct accctgcccg
    27901 gtgcgtaacc aagtttcggc agataagccg gactggtcta cagtgccgtt tatcatggcc
    27961 gatcagggac cagtacgccg gcgcattgag ttgtggtttc gacgcaacaa aatcagcaac
    28021 ccgatgattt acgccacggt cggcgggcat gaagcgatgg tgtcgatggt ggcgctcggc
    28081 tgtggcgtag ccttgttgcc ggaagtggtg ctggaaaaca gcccggaacc ggtgcgtaac
    28141 cgcgtgatga ttttagagcg cagtgatgag aaaacgccgt ttgaactggg cgtgtgtgtg
    28201 cagaaaaagc ggctacatga gccgctgatt gaggcattct ggaagatttt gccgaaccat
    28261 taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca atcgtaaccc
    28321 aactcattca accgggtttc gaaatccggt tcatggtcgc caagttcgaa cgccgccagt
    28381 acgcgcccgt agtcggtgcc atggctgcga tagtggaaca aagaaatgtt ccagtgcgta
    28441 cccagcgtgt tgaggaagcg caacagcgcg cccggcgatt ccgggaattc gaagctgtag
    28501 aggcgttcct gcaacggatg cgacggacgc ccgccgacca tatagcgcac atgcagcttc
    28561 gccatttcgt cgtcggagag atccactacg ctgtagccgc catcgttgag catctgcaaa
    28621 atttctttgc gctcttcgag gccacggctc aggcgcacac cgacaaagat gcaggcgttt
    28681 ttggcatcgg caaaacggta gttgaactcg gtgaccgagc gcccgccaag cagctggcag
    28741 aacttcagga agctgccctt ctcttccggg atggttaccg ccagcaacgc ttcacgctgt
    28801 tcgcccagtt cgcaacgttc cgagacgtag cgcaggccgt gaaagttcac gttagcacca
    28861 gaaagaatat gcgccagccg ctcaccgcga atgttgtgct gggcgatata ttttttcatt
    28921 cccgccagtg ccagcgcgcc agagggttcc gctaccgcgc gcacatcttc gaacaggtct
    28981 ttcatcgccg cacatatggc atcgctatcg acggtgatga tgtcgtcgag atactcctgg
    29041 cataaacgga aggtttcgtc gccgatgcgt ttcaccgcaa caccttcggc aaacagcccc
    29101 acgcgtggca gatcaaccgg atgacctgca tccagcgccg ctttcaggca ggcggagtct
    29161 tccgcttcca cggcaatcac tttgatttgc ggcatcagtt gtttgatcag taccgccacg
    29221 cccgccgcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg ggcgtcctgc
    29281 tggagcagtt ccagcgccag cgtgccttgc ccggcgatca ccatcggatg gtcgaacggc
    29341 ggcacccagg tgaagccctg ctgctgtgac agttcgatcg ctttcgcttt tgcttcatca
    29401 aagttcgcgc cgtgaagaag cacttcgccg ccgaagccgc gcaccgcgtc gactttgata
    29461 tcggcggtgg cggttggcat gacgatcagt gccttcacgc ctaaccgtgc ggaagaaaac
    29521 gcgacgccct gcgcgtggtt acccgcagaa gcggtgatca cgccgtgcgc tttctgttct
    29581 tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct taaagctatg aactggctga
    29641 cggtcttcgc gtttcaccag aatcacgtta tcgagacgcg acgagagttt ttccattttt
    29701 tgtagcggcg tgacctgcgc cgcttcgtag accggcgcgc gcagcaccgc tcttaaatat
    29761 tcggcgcctt ccggggcgcc ggagagaggt tgtgagtctg ccatcattaa ccccccagtt
    29821 tcgatttatc acgcaccgcg cctttgtcgg cgctggttgc caggctggcg taggcacgca
    29881 gcgcaaagga aacctgacgt tcacgatttt tcggtgtcca ggctttgttg ccacgagctt
    29941 cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga atgccacggt
    30001 tcggaatgtc gatagcgatc aagtcaccat cttcaatcag gccaatgctg ccgccacttg
    30061 ccgcttccgg tgagacgtgg ccgatggaaa gaccagaggt gccgccagag aaacgaccgt
    30121 cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaaactg gttgggtaga
    30181 gcatttcctg catccccgga ccgcctttcg ggccttcata gcgaattact accacatctc
    30241 cggcgacaac tttaccgccg agaatcgctt ctaccgcgtc gtcctggctt tcgtagactt
    30301 tcgccgggcc agtgaatttg aggatgctgt catcgacgcc tgcggtttta acgatgcagc
    30361 cgttttctgc gaaattaccg tagagtaccg ccaggccgcc gtctttgctg taagcgtgtt
    30421 ccagcgagcg gatacagcca ttggagcgat cgtcgtccag cgtatcccag cggcaatcct
    30481 gcgagaatgc ctgtgtagta cgaatgcccg ccgggccagc gcggaacata ttttttaccg
    30541 catcatcctg ggtcacaatg atgtcgtatt gttccagcgt ttgcggcaac gtcaggccaa
    30601 gtacattttt cacatcacgg ttcagtaacc ccgcacgatc cagctcgccg agaataccga
    30661 taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc ggcgcgactt
    30721 tacacagctg cggtactttg cgggagagct tatcgatatc actcatggtg aagtcgattt
    30781 ccgcttcctg cgccgccgcc agcaggtgaa gaacggtgtt ggtagagccg cccatcgcga
    30841 tatccagcgt catggcgttt tcaaacgccg ccttactggc gatgttacgc ggcagtgcgc
    30901 tttcatcatc ctgttcgtag taacgtttgg tcaattcaac aatgcgttta ccagcattga
    30961 ggaacagttg cttgcgatca gcgtgggttg ccagcagcga gccgttgccc ggctgcgaca
    31021 ggcccagcgc ttcggtcagg cagttcattg agttagcagt aaacatcccg gagcaggaac
    31081 cgcaggttgg gcaggcggat cgttcaacct gatcgctctg ggagtcagaa actttcgggt
    31141 ctgcgccctg gatcatcgca tcaaccagat cgagcttaat gatctgatcg gacagtttgg
    31201 ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc aggcgcaggg
    31261 aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc atggcgtcgg
    31321 cgcagtgggc gttgaccata tattcaacgg aatcagcgat cagttcgcga gatggcagtg
    31381 aataaagcat gcccccgtgg cccatagcga tcccatcatc caccgcaatg gtgttgaact
    31441 ctttggcaac gccgcccgcc gcttcaattt gttcggcgac cagtttaccg agatcgcgca
    31501 gatgaacgtg acccggtaca aattgggtga acgagttcac aaccgcgata atcggcttac
    31561 cgaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc gccatattac
    31621 gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc ccagtgtctg
    31681 tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt aacttgatct
    31741 aaccagcccc atttatcttc ggtctcgcca gtgaagaggc cgaagaaggc ttgctgaatg
    31801 cgtttggtaa ccgggccgca acggccttca ccaacctgaa taccatctac actgcgcact
    31861 ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaggta cagggattcg
    31921 cgcgacagca cctgctcacg cacttcaatt cccagctctt tcgccagttt gatgatggca
    31981 tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gagtaaacag cacaccgtct
    32041 ttcacttcaa acaggttttc gcctgcgcct tcagagatat aaccattcac atccaacgcg
    32101 ataccttcct gataaccgtg gcggcgcgct tcgctaccca ccaacaggga agagaggtag
    32161 ttaccaccag ctttcgctgc ggttgggatg gtgtttggtg cagcacggtt ccaggaggaa
    32221 accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgcccc ccacgggaaa
    32281 gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttacgcccat gccaacatca
    32341 ccgacgaaaa tcagcggacg gatataggcg ctggtgagat tgtttttgcg gatcacgtcg
    32401 cgacaagctt ccatcagctc atcaatgctc tgcgaaaccg ggaaacgata gattttggcg
    32461 gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc tttgtgcgag
    32521 tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg cgacatcaca
    32581 tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta atcagctttc
    32641 ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg atgtggttgt
    32701 gctctggcag atagcaacgt gtgcgacgtc caccagttta tttaactgac taaacagtaa
    32761 gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg catcgctggc
    32821 ggcagccata ttcattgagc agacgtggaa accacgatga cgcaccacgc gtaaaacacg
    32881 ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca tcatgataat
    32941 ttctccaaca tttcagaatt actggcacca ggcggcacca gcggccagac gttctcaagt
    33001 tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt gtcgagtgcc
    33061 gcttcaacct ggtctttatg ggtgatgtgt tggccaggga tgccgaaggc gctggctaac
    33121 atgaggaaat cggggttatc ggtaagggtg gtttcgctgt atcgttcctg gaaaaacagt
    33181 tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat tttcaacggt
    33241 aactgcttgc gttttacggt gcccagctct tgcacattca tcataaaaga gccgtcaccg
    33301 gagatacaga cgacagtatc gttcggtcgc gcgacttgtg cgccaaccgc cgccggtaaa
    33361 ccgaaaccca tggtgcctaa gccgctggag gtgatgaagt tttccgggcg agtgtggacg
    33421 atgtgctggg cagcccacat ctggtgctgc cccacatctg tggtcacgat gcaatccgca
    33481 ggtttacgat ccgacagttg ttttaacaac aacggcgcgt agatagcgtc accaggatgg
    33541 tcgtaacgcc aggcatgttc atcacgcagt tgcgcgcagt gttgctgcca gtcatcgata
    33601 tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccctgtaa tgccacatgt
    33661 gcctgacgca gcttgttcat ttctgccgga tcgatatcca tatggataac gctggcgtgt
    33721 ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcgcc cacggctatc
    33781 agtagatcgc actcctgcac tgcgaagttc gccgctttgg tgccgtgcat ccccagcatg
    33841 cccagatagt acggataatc tgcttctact gcgcccagcc ctttcaacgt acaggtggca
    33901 ggcatttttg tggcagcgag aaattcacgc aaagccgaaa ctgcctgcgc catgcccacg
    33961 ccaccgccaa cgtacagcat cggtttttgc gcttttgcca gcatctgacg cgcttgctcg
    34021 acttcggcat gtgggaaagt cacttcgttt tcaacagtgg tgaaccacgg ttccagatcg
    34081 ccgctggcta actggatatc ttttggaata tcgaccaaaa ccggaccagg acgaccagag
    34141 ctggcgacgt caaacgcttc ggccatgata cgcggtaact cttccagtga ttgcaccagg
    34201 aaactgtgct tggtgcaggc taacgacaac cccagaatat ccacctcctg aaatgcgtca
    34261 gtgccgataa acggtgcgga tacctgaccg gtgatagcaa caacggggat ggaatctaac
    34321 agcgcgtccg caagcccggt tatcaggttg gttgcacctg ggccagacgt ggcgatgcaa
    34381 acgccagttt tgccggtagc acgggcataa ccgatagccg ccattgctgc accttgctcg
    34441 tgtcggcaca gcaagtgttc cacgccaccg tcatacaatg catcgtaaac cggcataatt
    34501 gcgccacccg gataaccgaa aacggtgttt acaccctgtg cccgcaacgc atgtaccacc
    34561 cactgtgcac cattcatagt tagttccccg tcctgaatct tgagaaacag aattttgtgc
    34621 tgttattcat tgtctgctcc tcagttatgt ttttaaggtc aaaaaaaacc cccggacctt
    34681 tcggtgcggg ggtcttagtt cgttaaggct tgatctctaa gcctttcctc gtccaagtgc
    34741 agccccgcat ggtgggataa taatcaccac cacgctaatc acgaccaggc taatcactcg
    34801 tagaagggct gtcattttgt cttttcttgc atcttgttcg aaggaatgcc taaagagtta
    34861 ccatagattt tgcgaatagc acaagatatt ttttggccga tttttatgat gtc
//
LOCUS       DACADF010000035        34863 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.035, whole genome shotgun sequence.
ACCESSION   DACADF010000035 DACADF010000000
VERSION     DACADF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.035"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..1970
                     /locus_tag="ISB30_001621"
     CDS             <1..1970
                     /locus_tag="ISB30_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAP1989262.1"
                     /translation="AGSQAGTVRVTAKVQGKTFTLSIKQTATTEPDSEVSAVLTAAPA
                     EQVVGYNINLQLAVKDSQGNAITGDNTLSFYALNQAEGVEFGTVTEKDGVYSATVTSK
                     RAGKITIGVKSDSHDFSGVKKEITFIEDRQQVTFSQFEASQNNALADGKQRNMVTVSL
                     ADRFGNVVPGYAVTLSLPAGITQVGGEHAVSTDENGNAIFALISSTPGSYVITAHAGS
                     QMSTELTVTFASNMTGASLSLTPESSSLISNIPANGKDAAVLDVQLTNTNASVNGQKI
                     QLITSSEGLSVPTNIVTDSTGHVSVPLTTVKAGEYTVTARVTDGSHSVESGSVKLTFV
                     PDVASAELNMSVSKQEIVADGSENATVNIQLVDANNNAFTGEVDLTITPSTGASLTSS
                     NLQLDAHGQATTQFTASKSGQYTIQAEYVLDGKRITASQNIDAVTDVKGAVLEITSSA
                     SSAVVSDTNNLVFTLLLKSKSGEALSGRALNVKTSGPSKYGALVVDKTTVTTDENGQA
                     TVSVHGRTVGSYKLTATLNELGSDVSAVKSFSLYADDANGVLTLTKDPGYETNDGSPV
                     GIYARFVDHFGNPLSGTVEFSAGSEDSPDSQRVKMEPATVTLHWTGNAASEFSTYESG
                     YHWIKAKITNSKNTYEKTIRTYVVKLPEKDS"
     gene            complement(2007..3050)
                     /locus_tag="ISB30_001622"
     CDS             complement(2007..3050)
                     /locus_tag="ISB30_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1989263.1"
                     /translation="MKINKILSSATLLYGMSMAMSVGSCATPVQTNLPGYTPGADIIS
                     VSPTRNQVDLIGDVVYSQIKGTRSVRQLHMSVLVPRTNDLKPAIIYYPGGGFMSSEHD
                     KFIEMRMALAEAGFVVAAVEYRTIPDTFPAPVEDGKAAIRYLREHASNYGIDPQRIGV
                     LGDSAGGWLAQMMGTTNGDKTFDKGDFLQQSSDVQAVATLYGISDLLNIGEGFPESVQ
                     EVHRSPAVTEALMINGPAFRNFAGAPITASKEKALNASPIGHMKGVKPPFLIMHGSKD
                     TLVSPEQSAKLFRMLKKNGDNAEYVLVEGAEHGDKTWYQPIIINRVVEWFTKNLGAPI
                     KTAPQQQNPNANL"
     gene            3400..3501
                     /locus_tag="ISB30_001623"
     CDS             3400..3501
                     /locus_tag="ISB30_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989264.1"
                     /translation="MRTHNPNSLLPSQMQKCTCVFLHLASDLCGGEA"
     gene            3498..3953
                     /gene="ybcN"
                     /locus_tag="ISB30_001624"
     CDS             3498..3953
                     /gene="ybcN"
                     /locus_tag="ISB30_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HAP1989265.1"
                     /translation="MNLPQDGIKLHRGNFIAIGQQIQPYLENGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            3953..4123
                     /locus_tag="ISB30_001625"
     CDS             3953..4123
                     /locus_tag="ISB30_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989266.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            4116..4406
                     /locus_tag="ISB30_001626"
     CDS             4116..4406
                     /locus_tag="ISB30_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAP1989267.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIKA"
     gene            4403..4765
                     /locus_tag="ISB30_001627"
     CDS             4403..4765
                     /locus_tag="ISB30_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP1989268.1"
                     /translation="MNTYCITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAIPVKISIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            4762..4902
                     /locus_tag="ISB30_001628"
     CDS             4762..4902
                     /locus_tag="ISB30_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HAP1989269.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPSSVLTDYRILKNYAKTLKGA
                     AA"
     gene            4899..5588
                     /locus_tag="ISB30_001629"
     CDS             4899..5588
                     /locus_tag="ISB30_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAP1989270.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQLYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     IFSQQDIAKLD"
     gene            5910..6215
                     /locus_tag="ISB30_001630"
     CDS             5910..6215
                     /locus_tag="ISB30_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP1989271.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            6202..6678
                     /locus_tag="ISB30_001631"
     CDS             6202..6678
                     /locus_tag="ISB30_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HAP1989272.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTLRESEV"
     gene            6895..7077
                     /gene="rzoD"
                     /locus_tag="ISB30_001632"
     CDS             6895..7077
                     /gene="rzoD"
                     /locus_tag="ISB30_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HAP1989273.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(7168..7461)
                     /gene="iss"
                     /locus_tag="ISB30_001633"
     CDS             complement(7168..7461)
                     /gene="iss"
                     /locus_tag="ISB30_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HAP1989274.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            7942..8268
                     /locus_tag="ISB30_001634"
     CDS             7942..8268
                     /locus_tag="ISB30_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HAP1989275.1"
                     /translation="MKRRLLLLFLLSVLAVGCSQQKADEPRQLVTVYPRYPEYAAANY
                     IKGLVEVKFDIGADGTVTRIVFLRSEPHNLFRDEVVKAMAKWRFEKNRPCQGVKRQFI
                     FTPSRP"
     gene            8475..8657
                     /locus_tag="ISB30_001635"
     CDS             8475..8657
                     /locus_tag="ISB30_001635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989276.1"
                     /translation="MLAVFQLVKNTCSRLLQCLQHAAAMRLAFTNHLSFLYQSLRELI
                     MEYQVNADVIGFGYAA"
     gene            9221..9766
                     /locus_tag="ISB30_001636"
     CDS             9221..9766
                     /locus_tag="ISB30_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP1989277.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     DVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            9741..11666
                     /locus_tag="ISB30_001637"
     CDS             9741..11666
                     /locus_tag="ISB30_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HAP1989278.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHCMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLHVDEAINKTYT
                     RRNGAEMSVSRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            11663..11869
                     /locus_tag="ISB30_001638"
     CDS             11663..11869
                     /locus_tag="ISB30_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lambda prophage-derived head-to-tail joining
                     protein W"
                     /protein_id="HAP1989279.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            11866..13467
                     /locus_tag="ISB30_001639"
     CDS             11866..13467
                     /locus_tag="ISB30_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP1989280.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRDRLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            13448..14767
                     /locus_tag="ISB30_001640"
     CDS             13448..14767
                     /locus_tag="ISB30_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HAP1989281.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAVAPQSAQARSDTALDRLMQGAPAPLAAGNLASDAVNDLLNTPV"
     gene            14777..15109
                     /locus_tag="ISB30_001641"
     CDS             14777..15109
                     /locus_tag="ISB30_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HAP1989282.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            15165..16190
                     /locus_tag="ISB30_001642"
     CDS             15165..16190
                     /locus_tag="ISB30_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HAP1989283.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLV
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQLEGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            16232..16630
                     /locus_tag="ISB30_001643"
     CDS             16232..16630
                     /locus_tag="ISB30_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAP1989284.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVTLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            16642..16995
                     /locus_tag="ISB30_001644"
     CDS             16642..16995
                     /locus_tag="ISB30_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1989285.1"
                     /translation="MADFDNLFDAAIVRADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISTDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            17007..17585
                     /locus_tag="ISB30_001645"
     CDS             17007..17585
                     /locus_tag="ISB30_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1989286.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIRLGNARVVLSRRRRRKKGQ
                     RSSLKGGDSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            17582..17977
                     /locus_tag="ISB30_001646"
     CDS             17582..17977
                     /locus_tag="ISB30_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1989287.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDVPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            17985..18725
                     /locus_tag="ISB30_001647"
     CDS             17985..18725
                     /locus_tag="ISB30_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1989288.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELSAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            18741..19163
                     /locus_tag="ISB30_001648"
     CDS             18741..19163
                     /locus_tag="ISB30_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAP1989289.1"
                     /translation="MFLKTESFEYNGVTVTLSELSALQRIEHLALMKRQAEPAGSDSN
                     RQVTVEDVIRTGAFLVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQAEDAGPAEPVSAGKCSTVS"
     gene            19145..19579
                     /locus_tag="ISB30_001649"
     CDS             19145..19579
                     /locus_tag="ISB30_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HAP1989290.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            19572..22133
                     /locus_tag="ISB30_001650"
     CDS             19572..22133
                     /locus_tag="ISB30_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP1989291.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYAARQEELNKALKDGKILQADYNTLMAAAKKDYETT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSVQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            22130..22459
                     /locus_tag="ISB30_001651"
     CDS             22130..22459
                     /locus_tag="ISB30_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1989292.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWQQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            22459..23157
                     /locus_tag="ISB30_001652"
     CDS             22459..23157
                     /locus_tag="ISB30_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAP1989293.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            23162..23905
                     /locus_tag="ISB30_001653"
     CDS             23162..23905
                     /locus_tag="ISB30_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1989294.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPKGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGEIHK
                     FRCVPHLTGRCFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLAAASTFV"
     gene            23842..24444
                     /locus_tag="ISB30_001654"
     CDS             23842..24444
                     /locus_tag="ISB30_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAP1989295.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDTGETELSARLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKPRTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            24505..27987
                     /locus_tag="ISB30_001655"
     CDS             24505..27987
                     /locus_tag="ISB30_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAP1989296.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLLNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKETIVDNGAHFDGEQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVEASARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PTFSLTPDGRLSARNADISGNVNANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVG
                     KAFPRNGSYASGTITVTVYDDQAFDRQIVVPPVLFRGGKHENFNSNNQQSYWYSTCKL
                     QVLKNGQEIFQQPATDVSRVFSSVIDMPAGHGHVTLTFNVSSYGANNWTPTTSISDLL
                     VVVMKKSTAGISIS"
     gene            28046..30067
                     /locus_tag="ISB30_001656"
     CDS             28046..30067
                     /locus_tag="ISB30_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1989297.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKAKRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVALLVEGFPPSHAGTITVYEDSKPGTLNDFLGAATEDDVCPEA
                     LYRFEKMVEEAARNAEAASQSAAAAKKSETAAASSRNAAKTSETNAGNSAKAAASSKT
                     AAQNAATAAERSETNARASEEASADSEEASRRNAESAAENAGVATTKAREAAADATKA
                     GQKKDEALSAATRAEKAADRAEAAAEVTAEPCANIVPPLPDVWIPFNDSLDMITGFSP
                     SYKKIVIGDDEITMPGDKIVKFKRASTATYINKSGQLKLAEVDEPRFERDGLLIEGQR
                     TNYLRNSNKPDSWTVHSALNKTFGTDKQGFNYATVTPTESIVGTTGGYTVHGVVAADR
                     FPLASGECFTFSCRVKGAKARCRLRVSVIIGGTDTFSADSYLDLDTRIATVSGNTSLI
                     TAKAEQQGEWTYYEATYTANTDIDTVNCAFYMTNKISNETFYDDSTLTMTTPQIELGN
                     TASSFIVTTMPTTRASDVVTIPSQNNLSTRPFTVLCEVSRNWSTPPNVAPRIFDVGGH
                     SIDDNYLSLGFVATGKISANVGMVQPQIASDGERFIVGVRAKSDLSVNAICNGNYTTN
                     LNGKIFGVTATSYRFGGQTAAGTRHLFGHIRNFRVWFKELNDRQIKEAV"
     gene            30064..30342
                     /locus_tag="ISB30_001657"
     CDS             30064..30342
                     /locus_tag="ISB30_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989298.1"
                     /translation="MKDLTLKFPGNREFKSFLSSLDWEEDEDLQNKLLVDEIGFTYTE
                     TGVTEEGEPVCVRNDGYFVNIRILDDLFDVSVFSDYVVELETPLREWS"
     gene            30355..30648
                     /locus_tag="ISB30_001658"
     CDS             30355..30648
                     /locus_tag="ISB30_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989299.1"
                     /translation="MDISPLLHALCAVAAQILVGLFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEMFGHGKRMNMPWWGGFDPRAWDVASLMDFAVPVVACLLVWLLVNRG"
     gene            complement(30740..31597)
                     /gene="sitD"
                     /locus_tag="ISB30_001659"
     CDS             complement(30740..31597)
                     /gene="sitD"
                     /locus_tag="ISB30_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAP1989300.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVLFIMTFAVTSINARKKGNS
                     HTQDLLSPN"
     gene            complement(31594..32451)
                     /gene="sitC"
                     /locus_tag="ISB30_001660"
     CDS             complement(31594..32451)
                     /gene="sitC"
                     /locus_tag="ISB30_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAP1989301.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(32448..33275)
                     /gene="sitB"
                     /locus_tag="ISB30_001661"
     CDS             complement(32448..33275)
                     /gene="sitB"
                     /locus_tag="ISB30_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAP1989302.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEDVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(33275..34165)
                     /gene="sitA"
                     /locus_tag="ISB30_001662"
     CDS             complement(33275..34165)
                     /gene="sitA"
                     /locus_tag="ISB30_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAP1989303.1"
                     /translation="MLLGGLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
BASE COUNT         8671 a         8339 c         9936 g         7917 t
ORIGIN      
        1 ctgctggttc gcaggctggt acggtacgtg taactgcaaa agttcaggga aaaacattta
       61 ctctcagtat taagcaaaca gcaacaacag aacctgattc tgaggtcagt gctgttttaa
      121 ccgctgcacc agcagaacag gtagttggtt acaatatcaa tctacaactg gcagtgaagg
      181 attcacaagg aaatgcaatc actggtgata acactctgag cttttatgct cttaaccagg
      241 cagaaggagt cgagtttggg actgtaacgg aaaaagacgg tgtctatagc gcgacagtaa
      301 cttcaaaacg agctggtaag attactatcg gtgttaaatc tgatagtcat gatttctcag
      361 gtgttaagaa agaaatcacc tttattgagg accggcagca ggttacattc tctcagtttg
      421 aagccagcca aaataatgca ttggctgatg gaaaacagag aaatatggta actgtaagcc
      481 ttgcggaccg tttcggcaat gttgttcctg gttatgctgt cacattgtct ttaccagcag
      541 ggataactca agttggtggt gaacatgctg tttcaactga cgaaaatggt aatgctattt
      601 ttgcgttaat aagctccact ccggggtctt acgtgattac agctcatgct ggttctcaga
      661 tgtctactga gttaacagtg acctttgcat caaatatgac cggagcttca ctgtcactga
      721 cgcctgaaag cagtagttta atttccaata ttcctgctaa tggtaaggat gcagcggtat
      781 tagatgtgca gctgacaaat acgaatgcgt cggtcaatgg gcaaaagatt cagctcatca
      841 cctcatctga aggattatct gtaccaacaa atatcgtgac tgattcaaca gggcatgttt
      901 ctgttccgct tacaacggtg aaagctggtg agtacacggt gactgcacgg gtgactgacg
      961 gttcccatag cgtggaatct ggcagcgtta agttaacttt tgtgcccgat gttgcttctg
     1021 cagaactgaa tatgagtgta tcaaaacagg agattgtggc cgatggcagt gaaaacgcaa
     1081 cagtaaacat tcagttggtt gatgcgaata acaatgcgtt tacaggcgag gtggatttaa
     1141 cgattacacc atcgacggga gcatcattga ccagtagtaa tctgcaactg gatgctcatg
     1201 gacaggcaac aacgcagttc actgcatcga aatcaggcca gtacacaatt caggccgagt
     1261 atgttctgga tgggaaacgc ataaccgcaa gccagaatat tgatgccgtg acggatgtga
     1321 agggggctgt gttggagatc acatcatctg catcttcggc tgtagtgagc gataccaaca
     1381 atctggtatt tacgttactg ttgaaaagta agtcaggcga agcattaagt ggtcgagcat
     1441 tgaatgttaa gacatctggg ccgtctaaat atggtgcgtt ggtggttgat aaaaccacag
     1501 taacgactga tgagaacggt caggctacgg taagcgtaca tggtcgaacg gttggttcgt
     1561 ataaattaac ggcgacctta aacgaacttg gcagtgatgt tagcgcggtg aaatcattct
     1621 ccctgtacgc tgatgacgca aatggagtat tgactctaac aaaagatcct ggttatgaga
     1681 caaacgatgg atctccagta ggtatttatg ctcgatttgt cgatcatttt ggaaacccat
     1741 tatctggtac ggttgagttt tctgctggta gtgaagatag tccagactct caacgggtca
     1801 aaatggagcc tgctacagta acgttacact ggacaggtaa tgcagcttcg gaattcagca
     1861 cctatgaaag tggttatcac tggattaagg ccaaaataac gaatagcaaa aatacatatg
     1921 agaaaacaat aaggacttat gttgtaaaac tacctgaaaa ggatagttaa caaaattgaa
     1981 tggggagggc ttagccctcc ctctttttac aggttagcgt ttgggttttg ttgttgggga
     2041 gctgttttta taggcgctcc caggttttta gtaaaccact caacgactct gtttataata
     2101 attggctgat accatgtctt atcgccatgc tcggcccctt ctaccagcac gtactcagcg
     2161 ttatcaccgt tcttcttcaa catcctgaat agtttggcgc tttgctcagg tgaaaccaga
     2221 gtgtctttgc taccatgcat aataagaaat gggggtttta ctcctttcat atgtccgatt
     2281 ggactggcgt ttagcgcttt ttcttttgac gctgtgatgg gggctcccgc aaaatttctg
     2341 aatgcagggc cattgatcat taaggcttcg gttacggcag gagagcgatg aacctcctgc
     2401 actgattcag ggaacccctc gccaatattc aacaagtcag aaatcccata aagtgtggca
     2461 actgcctgaa catctgagga ttgctgaaga aagtcacctt tatcaaaggt tttgtcacca
     2521 tttgtagttc ccatcatctg ggcaagccat ccaccggcag agtcacccag aactccgatt
     2581 ctttgaggat caatcccata attgctggca tgctctctca ggtaacgtat tgcagctttt
     2641 ccatcctcaa ctggtgctgg aaatgtatca ggaattgttc tgtattctac agcggccaca
     2701 acaaaaccag cttctgccag agccattctc atttcaataa atttgtcatg ttcagaagac
     2761 atgaatccgc cgccgggata ataaataatg gctggtttta aatcatttgt tcgcgggaca
     2821 agaactgaca tgtgaagctg tctgacagaa cgagttcctt ttatctggga ataaacaaca
     2881 tcaccaatga gatcgacctg gtttctggtc ggtgaaacac taatgatatc tgcacccggg
     2941 gtgtaaccag gaagattagt ctggacaggt gttgcacaac tcccgaccga catggccatt
     3001 gacataccat acaatagtgt tgcagatgat aatatcttgt taattttcat gttttacatg
     3061 ccttgactta tcggatgaaa ttctcttact aaatttaacg gcagtttaat agccgtacaa
     3121 ctcgtggtag ccagggaaat atcccagtcg tggttaaggt tgtacttgac ggctattatt
     3181 tcaacaatgc ttaaagtgag agcttaaggt tatgcgtatg ttttacagtc cgagtagatt
     3241 ctgaccaata gcttctttgt ataattctgt cagaaataaa tcttggcttg catgaagttt
     3301 gagaccttat cttgtttgat tatgaataat caattcgcca taattgtatc accggagcct
     3361 gaacaactcc ggtgacttct gcgctaaacg gggacgttta tgcgcacaca caatccaaac
     3421 tctcttctcc cttcacagat gcagaaatgc acctgcgttt ttttgcatct agcgtctgac
     3481 ctctgtggag gtgaagcgtg aacctaccac aagatggcat caaactgcat cgcggtaact
     3541 tcatcgctat cggccagcag atccagcctt atctggagaa cggaaaatgc tttcgcatgg
     3601 tgcttaaacc gtggcgtgag aaacgcagtc tttcccagaa tgcactcagc cacatgtggt
     3661 acagcgaaat cagtgaatac ctcatcagca gggggaaatc gttcgctacc gcagcatggg
     3721 taaaagatgc tctcaaacac acatacctcg gttatgaaac caaggacctg gttgatgtcg
     3781 taaccggcga aatcactact atccagtcgt tacgccatac ctccgatctc gataccggag
     3841 agatgtatgt cttcctgtgt aaggttgaag cctgggcggt gaatattggc tgccacctga
     3901 ctattccgca gagctgcgag ttccagctgc tgcgcgacaa gcaggaggcg taatggctac
     3961 accgcttatt cgtgtcatga acggacacat ctacagagta ccaaatcgtc gtaagcgtaa
     4021 gcctgagctg aaaccatccg aaataccaac actgctcgga tataccgcca gcctggttga
     4081 taaaaaatgg ttgcgactgg cagcaaggag gaatcatggc tgacttgaga aaagcagcgc
     4141 gtggtcggga atgccaggta agaatccctg gcgtatgtaa tggcaatcct gaaacgtcag
     4201 tactggcaca tatccggctg gctggattgt gcggtaccgg tatcaaaccg ccagacctga
     4261 ttgccaccat tgcatgttct gcctgccacg acgaaatcga ccgtcgcacg cattttgttg
     4321 acgctggata tgcaaaagaa tgtgcgctgg aaggtatggc gagaacacag gttatctggc
     4381 tgaaagaggg ggtaattaag gcgtgaatac ttactgcatc acactaccct ggccgccgag
     4441 caataatcgc tattaccgcc ataatcgcgg acgcacgcat atcagcgcag aggggcaggc
     4501 ataccgcgat aatgtcgccc gaatcattaa aaacgcaatg ctggatatcg gcctggctat
     4561 tcctgtgaaa atcagcattg agtgccacat gccggatcgc cgtcgccgtg accttgataa
     4621 tctgcaaaag gccgcttttg acgcactcac caaagcaggt ttctggctgg atgatgctca
     4681 ggtcgttgat taccgcgttg tgaagatgcc cgttaccaaa ggtgggaagc tggagctgac
     4741 tatcactgaa ctgggagatg aatgatgttt gagtcttata tggcagaacg tcttcgccgc
     4801 cgctgggtgc gcctgcgctt atatcgtttt cctagttctg ttttgaccga ttaccggata
     4861 ctgaagaatt acgccaaaac actgaaagga gctgccgcat gaatacccaa tatttacagt
     4921 atgtccgcga gcaactcatt gtggctaccg ctgatttgag cggagcaacg aaaggacagc
     4981 ttgaagcctg gctggagcat gcacaatttg atactggtac atacaaacga aagaagccgc
     5041 gcattctgga tgaggtaact ggcaggatga ttacgctgga taatccgccg atttccggta
     5101 agcagttgta cgcaaaaggt tcatccattg cactggtcag ccaggttgag ttctcaacct
     5161 cgtcatggcg ccgcgcggtt ctgtctctcg aagaacatca gaaagcgtgg ttgctgtgga
     5221 gttacagcga aagtgttcgc tgggaacatc aggtcaccat aacgcagtgg gcatggagcg
     5281 agtttaagac tctgttgggt accaggaaaa ttgcaggtaa aacactggaa cgtttgaaga
     5341 agttgatctg gctggcggca caggatgtga agaacgagct ggcagggcgt aagacctatg
     5401 aataccagga gctggcatca ctggtgggag tgacatcaaa aaactggtct gagacattta
     5461 ctgaacgctg ggttgcaatg aagcacattt ttctacagct tgatagccaa gctttattgc
     5521 ttttaacgaa aacacgttca aaacaaaaga ccatattttc acagcaagat attgcaaaac
     5581 tggattaaaa atcatatatt ttatgtaaat ctgatatttt gccaatgttg tacgcactgg
     5641 cagtaatcca aattcaagcc cgaggtttaa agccttgggc ttttctgttt ctgaacggtg
     5701 agtagccttc caacctaccc cagccagggt gtcttcagct gttgagttga tattgcttaa
     5761 ccctctgttg ccagctacat gctggctttt ttattccagg cttgcgggga gcatcaactc
     5821 cgtgctttgt cgttaaatta ccccgtgagc ctgatttctg acatttaacg tcccggcctt
     5881 ttgtcggcgg cgaaacattg gctattcata tgcacgaaaa agagagcctt gccggagcgt
     5941 tctggctcgt tttgctgatc atcgcaggtt ggggcggtct ggtccgctac ctgatagatg
     6001 tgaagcagag taaagcaacg tggagttgga taaatgctct ggctcaaata gtggtatcag
     6061 gattcaccgg tgttattggt ggcctgatca gcatcgaaag tggattcagt atttacatga
     6121 ttctcgcgac agcggggatt agtggtgcga tgggttcggt tgcactgacg tacttctggg
     6181 aacgactgac aggggtgaaa aatgcaaaat cttaatcctc agcgtaaagc cttcctcgat
     6241 atgttggcgt ggtcagaagg aacggataac gggcgacaac cgacacgtaa ccacggttat
     6301 gatgttattg ttggcggcga actgtttact gattactccg atcaccctcg caaacttgtc
     6361 acgctacacc ccaaactcaa atcaacagcc gccggacgtt atcagcttct ttcacgctgg
     6421 tgggatgctt accgtaaaca gcttggtttg aaagacttct cccccaaaag ccaggacgca
     6481 gtggcattac agcagattaa agagcgtggt gctttaccga tgattgatcg cggtgatatc
     6541 cgtcaggcaa tcgaccgttg cagcaatatc tgggcttcac tgccgggcgc tggttatggt
     6601 cagttcgagc ataaggctga cagcctgatt gcaaaattca aagaagctgg cggaacgctc
     6661 agagagagtg aggtatgagc agaataaccg cgattatctc cgctctggtt atctgcgtca
     6721 tcgtctgcct ttcatgggcg gttaatcatt accgcgataa cgcgattacc tacaaagccc
     6781 agcgcgacaa aaatgccaga gaactgacgc tggcgaacgc ggcaattact gacatacaga
     6841 tgcgtcagcg tgatgttgct gcgctcgatg caaaatacac gaaggagtta gctgatgcga
     6901 aagctgaaaa tgatgctctg cgtgatgatg ttgccgctgg tcgtcgtcgg ttgcacatca
     6961 aagcagtctg tcagtcagtg cgtgaagcca ccaccgcctc cggcgtggat aatgcagcct
     7021 ccccccgact ggcagacacc gctgaacggg attatttcac cctcagagag aggctgatca
     7081 ctatgcaaaa acaactggaa ggaacccaga agtatattaa tgagcagtgc agatagagct
     7141 gcccatatcg atgggcaact catgcaatta ttgtgagcaa tacacacgcg cttccagcgg
     7201 agtataaatg cctaaagtaa taaaaccgag caatccattt acgaatgttt gctgggtttc
     7261 tgttttaaca acattttctg caccaccaca aatttttgct gcatcgacag ttttcttctg
     7321 cccaattccc gaaacgaaga aatgatgggt gatggtttcc tttggtgtta ctgctgtagg
     7381 tttgtttcca acagtaaacg tctgttgagc acatcctgta ataagcattg ccagagcggc
     7441 agaaaacaac atttttttca tcttattatc ctgcattgtt aaaaacggca gaatcctatg
     7501 tgacaacaat taaacgatag ttaaatggat tgatgaaaat taaaactaca caggtgggct
     7561 cagactattg gaggaagttg gggacactca gaatcctgtg gaatgaaata aaccggtcta
     7621 tccgtccatt acccttttag ctgcgctgta tcgtcgccgt attcccgcat taaccatgac
     7681 cgtagcccga cggggaattc cttctgcgtg agtgtgcggg aataattaaa aacgatgcac
     7741 accgggtttt taccgcgtta atgattcgcg ggtttatccc ggtgcgatgg tggaagaaac
     7801 aggaagctgt attacagaaa gtgctactac tgtatcccga tgcgatgtat gtaatgtgag
     7861 tcagataatg gcacaggatg tggtgatgtg gcagtctgga acacaggata tattgtcaga
     7921 ataagacccg taggaataaa aatgaaaaga cgccttttac tactttttct gttatctgtc
     7981 ctggcagtgg gatgctcgca gcaaaaagct gatgagcccc ggcaattagt gacggtgtat
     8041 ccacgatatc cggaatatgc tgcagcaaat tatatcaagg ggctggttga ggttaagttc
     8101 gatattggtg ctgatgggac tgtgacacgg atcgtttttc tccgctcaga gcctcataat
     8161 ttgtttcgtg atgaagtggt gaaggccatg gcgaaatggc gatttgaaaa gaatcgcccc
     8221 tgtcagggag tgaagagaca atttatcttt acgccgtcac gtccttgatg cttccaggta
     8281 gagaggggct ggaagcagga gaaaaatgaa agagccagcg gttatatttt tgtcatggct
     8341 gacgaggaat gatggaagaa ggcgttgtat gccacacaac gcctcactgt tcatttcttc
     8401 tttttctctg gtggaacccg atgaataaga gttgcactgg tttccgatga gatggcgata
     8461 tactcgggca aagtatgctg gcagttttcc aactggtcaa aaatacctgc tctcgtctgt
     8521 tgcaatgcct gcagcatgcg gcggcaatgc gccttgcttt tactaaccat ctttccttcc
     8581 tctatcagtc gctgcgtgaa ctcatcatgg aataccaggt aaatgcggat gttatcggtt
     8641 ttggctacgc agcatagtac aaaacggaca ggtgcatccc gggacggggg aggcgtcaca
     8701 tgtccctgtg atggttgttc cgggtaatgt actgtgtggg gcataaaaat gtccgataat
     8761 tttactttct accgcagtta gttgattcgt tggtcctggt agcacattgg gcgaggattt
     8821 aaatgccagg caactgaagg atgatgtcgc aagggagata gcgagaatat ttctgatttt
     8881 catttgatga tgcctctgtg tgaaatgacg gtaaacgacg cacttgtgcc ggcacataat
     8941 agcaagcacc ataatagatc agattcgatt cttgctgtaa gtgataatta ttctcgtttt
     9001 cgggtccttt ccgtcgatcc aacaggttac ggggcggcga cctcgcgggt tttcgctatt
     9061 tatgaaaatt ttccggttta aggcgtttcc gttcttcttc gccgtaactt aatgttttta
     9121 tttaaaacac tccctgaaaa gaaaggaaac gacaggtgct gaaaacgggc tttttggcct
     9181 ctgtcgtttc ctttctctgt ttttgtccgt ggaatgaaca atggaagtca acaaaaagca
     9241 gctggctgac attttcggcg cgagtatccg tactattcag aactggcagg agcagggaat
     9301 gcccgttctg cgaggcggtg ggaagggtaa tgaggtgctt tatgactctg ccgacgtcat
     9361 aaaatggtat gccgaaaggg atgctgaaat tgagaacgaa aagctgcgcc gggaggttga
     9421 agaactgcga caggccagcg agacagatct ccagccaggg actattgagt acgaacgcca
     9481 tcgacttacg cgtgcgcagg ccgacgcaca ggagctgaaa aatgccagag actccgctga
     9541 agtggtggaa accgcattct gtactttcgt gctgtcgcgg atcgcaggtg aaattgccag
     9601 tattctcgac gggatccccc tgtcggtgca gcggcgtttt ccggaactgg aaaaccgaca
     9661 tgttgatttc ctgaaacggg atatcatcaa agccatgaac aaagcagccg cgctggatga
     9721 actgataccg gggttgctga gtgaatatat cgaacagtca ggttaacagg ctgcggcatt
     9781 ttgtccgcgc cgggcttcgc tcactgttca ggccggagcc acagaccgcc gttgaatggg
     9841 cggatgctaa ttactatctc ccgaaagaat ccgcatacca ggaagggcgc tgggaaacac
     9901 tgccctttca gcgggccatc atgaatgcga tgggcagcga ctacatccgt gaggtgaatg
     9961 tggtgaagtc tgcccgtgtc ggttattcca aaatgctgct gggtgtttat gcctacttta
    10021 tagagcacaa gcagcgcaac acccttatct ggttgccgac ggatggtgat gccgagaact
    10081 ttatgaaaac ccacgttgag ccgaccatcc gcgatattcc gtcgctgctg gcgctggctc
    10141 cgtggtatgg caaaaagcac cgggataaca cgctcactat gaagcgtttt tccaatggtc
    10201 gtggcttctg gtgcctgggc ggtaaagcgg caaaaaacta ccgtgaaaag tcggtggatg
    10261 tggcgggtta tgatgaactt gctgcctttg atgaggatat tgaacaggaa ggctctccga
    10321 cgttccttgg cgacaaacgt attgaaggct cggtctggcc aaagtccatc cgtggctcca
    10381 cgcccaaagt gagaggcacc tgtcagattg agcgtgcagc cagtgaatcc ccgcattgta
    10441 tgcgttttca tgttgcctgc ccgcactgcg gggaggagca gtaccttaaa tttggcgata
    10501 aagagacgcc gtttggcctc aaatggacgc cggatgatcc ctccagcgtg ttttatctct
    10561 gcgaacataa tgcctgcgtc atccgccagc aggagctgga cttcactgat gcccgttata
    10621 tctgcgaaaa gaccgggatc tggacccgtg atggcattct ctggttttcg tcatccggtg
    10681 aagagattga gccgccggac agcgtgacct ttcacatctg gacggcgtac agcccgttca
    10741 ccacctgggt gcagattgtc aaagactgga tgaaaacgaa aggagatacg ggaaaacgta
    10801 aaaccttcgt gaacaccacg ctcggtgaga catgggaagc gaaaatcggt gaacgtccgg
    10861 atgctgaagt gatggcagag cggaaagagc attattcagc gcccgttcct gaccgtgtgg
    10921 cttacctgac tgccggtatc gactcccagc tggatcgcta cgaaatgcgc gtatggggat
    10981 gggggccggg tgaggaaagc tggctgattg accggcagat tattatgggc cgccacgacg
    11041 atgaacagac gctgctgcat gtggatgagg ccatcaataa aacctatacc cgccggaatg
    11101 gtgcagaaat gtcggtatcc cgtatctgct gggatattgg cgggattgac ccgaccatcg
    11161 tgtatgaacg ctcgaaaaaa catgggctgt tccgggtgat ccccattaaa ggggcatccg
    11221 tctacggaaa gccggtggcc agcatgccac gtaagcgaaa caaaaacggg gtttacctta
    11281 cggaaatcgg tacggatacc gcgaaagagc agatttataa ccgcttcaca ctgacgccgg
    11341 aaggggatga accgcttccc ggtgccgttc acttcccgaa taacccggat atttttgatc
    11401 tgaccgaagc gcagcagctg actgctgaag agcaggtcga aaaatgggtg gatggcagga
    11461 aaaaaatact gtgggacagc aaaaagcgac gcaatgaggc gctcgactgc ttcgtttatg
    11521 cgctggcggc gctgcgcatc agtatttccc gctggcagct ggatctcagt gcactgctgg
    11581 cgagcctgca ggaagaggat ggtgcagcaa ccaacaagaa aacactggca gattacgccc
    11641 gtgccttatc cggagaggat gaatgacgcg acaggaagaa cttgccgctg cccgtgcggc
    11701 actgcatgac ctgatgacag gtaaacgggt ggcaacggta cagaaagacg gacggcgagt
    11761 ggagtttacg gccacttccg tgtctgacct gaaaaaatac attgcggagc tggaggtgca
    11821 gaccggcatg acacagcgac gcaggggacc tgcaggattt tatgtatgaa aacgcccacc
    11881 attcccaccc ttctgggacc ggacggcatg acatcgctgc gtgaatatgc cggttatcac
    11941 ggcggtggca gcggatttgg tgggcagttg cgggcgtgga acccaccggg tgaaagtgtg
    12001 gatgcagccc tgctgcccaa ctttacccgt ggcaatgccc gcgcagacga tctggtacgc
    12061 aataacggct atgctgccaa cgccatccag ctgcatcagg atcatatcgt cgggtctttt
    12121 ttccggctca gtcatcgccc aagctggcgc tatctgggca tcggggagga agacgcccgt
    12181 gccttttccc gcgaggttga agcggcatgg aaagagtttg ccgaggatga ctgctgctgc
    12241 attgacgttg agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcac
    12301 gcctttaacg gtgaactgtt cgttcaggcc acctgggata ccagttcgtc gcggcttttc
    12361 cggacacagt tccggatggt cagcccgaag cgcatcagca acccgaacaa taccggcgac
    12421 agccggaact gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct gggatattac
    12481 gtcagtgagg acggctatcc tggctggatg ccgcagaaat ggacatggat accccgtgag
    12541 ttaccgggcg gtcgtgcttc gttcattcac gtctttgaac ccgtggagga cgggcagacc
    12601 cgcggtgcaa atgtgtttta cagcgtgatg gagcagatga agatgctcga cacgctgcag
    12661 aacacgcagc tgcagagcgc cattgtgaag gcgatgtatg ccgccaccat cgagagtgag
    12721 ctggatacgc agtcagcgat ggattttatt cttggcgcga acagtaagga gcagcgggac
    12781 aggctgaccg gctggattgg tgaaattgcc gcgtattacg ccgcagcacc ggtccggctg
    12841 ggaggcgcaa aagtgcctca cctgatgccg ggtgactcac tgaacctgca gacggctcag
    12901 gacacggata acggctactc cgtgtttgag cagtcactgt tgcggtatat cgctgccggg
    12961 ctgggtgtct cgtatgagca gctttcccgg aattacgccc agatgagcta ctccacggca
    13021 cgggccagtg cgaacgagtc gtgggcgtac tttatggggc ggcgaaaatt cgtcgcatcc
    13081 cgtcaggcga gccagatgtt tctgtgctgg ctggaagagg ccatcgctcg ccgcgtggtg
    13141 acgttacctt caaaagcgcg cttcagtttt caggaagccc gaagtgcctg ggggaactgc
    13201 gactggatag gctccggtcg tatggccatc gatggtctga aagaagtaca ggaagcggtg
    13261 atgctgatag aagccggact gagtacctac gagaaagagt gcgcaaaacg cggtgacgac
    13321 tatcaggaaa tttttgccca gcaggtccgt gaaacgatgg agcgccgtgc agccggtctt
    13381 aaaccgcccg cctgggcggc tgcggcattt gaatccgggc tgcgacaatc aacagaggag
    13441 gagaagagtg acagcagagc tgcgtaatct cccgcatatt gccagtatgg cctttaatga
    13501 gccgctgatg cttgaacccg cctatgcgcg ggttttcttt tgtgcgcttg caggccagct
    13561 tgggatcagc cgcctgacgg atgcggtatc cggcgacagc ctgactgccg gagaggcacc
    13621 cgcggcgctg gcgttatccg ttgatgatga cggaccacga caggcccgca gttatcaggt
    13681 catgaacggc atcgccgtgc tgccggtgtc cggcacgctg gtcagtcgga cgcgagcgct
    13741 gcagccgtat tcggggatga ccggttacaa cggcattatc gcccgtctgc aacaggctgc
    13801 cagcgacccg atggtggacg gcattctgct cgatatggat acgccaggcg ggatggtggc
    13861 aggggcattt gactgcgctg acatcatcgc ccgtgtgcgt gacataaagc cggtatgggc
    13921 gctggccaac gacatgaact gcagtgcagg tcagttgctt gccagcgccg cctcccgacg
    13981 tctggtcacg cagaccgccc ggacaggctc catcggcgtc atgatggctc acagtaatta
    14041 cggtgctgcg ctggagaaac agggcgtgga aatcacgctg atttacagcg gcagccataa
    14101 ggtggatggc aacccctaca gccatctacc ggatgatgtc cgggaaacac tgcagtcccg
    14161 gatggatgca acccgccgga tgtttgcaca gaaggtgtcg gcatataccg gcctgtccgt
    14221 gcaggctgtg ctggataccg aggctgcagt gtacagcggt caggaggcca ttgatgccgg
    14281 actggctgat gaacttgtta acagtaccga tgcgatcacc gtcatgcgtg atgcactgga
    14341 tgcacgtaaa tcccgtctct caggagggcg aatgaccaaa gagactcaat caacaactgt
    14401 ttcagccact gcttcgcagg ctgacgttac tggcgtggtg ccagcgacgg agggcgagaa
    14461 cgccagcgct gcgcagccgg acgtgaacgc gcagatcacc gcagcggttg cggcagaaaa
    14521 cagtcgcatt atggggatcc tcaactgtga ggaagctcac ggacgcgaag aacaggcacg
    14581 cgtgctggcc gaaacccctg gtatgaccgt ggaaacggcc cgccgcattc tggcagttgc
    14641 accacagagt gcacaggcgc gcagtgacac tgcgctggat cgtctgatgc agggggcacc
    14701 ggcaccgctg gctgcaggta acctggcatc tgatgccgtt aacgatttgc tgaacacacc
    14761 agtgtaaggg atgtttatga cgagcaaaga aacctttacc cattaccagc cgctgggcaa
    14821 cagtgacccg gctcataccg caaccgcgcc cggcggattg agtgcgaaag cgcctgcaat
    14881 gaccccgctg atgctggaca cctccagccg taagctggtt gcgtgggatg gcaccaccga
    14941 cggtgctgcc gttggcattc ttgcggttgc tgctgaccag accagcacca cgctgacgtt
    15001 ctacaagtcc ggcacgttcc gttatgagga tgtgctctgg ccggaggctg ccagcgacga
    15061 gacgaaaaaa cggaccgcgt ttgccggaac ggcaatcagc atcgtttaac tttacccttc
    15121 atcactaaag gccgcctgtg cggctttttt tacgggattt ttttatgtcg atgtacacaa
    15181 ccgcccagct gctggcggca aatgagcaga aatttaagtt tgatccgctg tttctgcgtc
    15241 tctttttccg tgagagctat cccttcacca cggagaaagt ctatctctca caaattccgg
    15301 gactggtaaa catggcgctg tacgtttcgc cgattgtttc cggtgaggtt atccgttccc
    15361 gtggcggctc cacctctgaa tttacgccgg gatatgtcaa gccgaagcat gaagtgaatc
    15421 cgcagatgac cctgcgtcgc ctgccggatg aagatccgca gaatctggtg gacccggctt
    15481 accgccgccg tcgcatcatc atgcagaaca tgcgtgacga agagctggcc attgctcagg
    15541 tcgaagagat gcaggcagtt tctgccgtgc ttaagggcaa atacaccatg accggtgaag
    15601 ccttcgatcc ggttgaggtg gatatgggcc gcagtgagga gaataacatc acgcagtccg
    15661 gcggcacgga gtggagcaag cgtgacaagt ccacgtatga cccgaccgac gatatcgaag
    15721 cctacgcgct gaacgccagc ggtgtggtga atatcatcgt gttcgatccg aaaggctggg
    15781 cgctgttccg ttccttcaaa gccgtcaagg agaagctgga tacccgtcgt ggttctaatt
    15841 ccgagctgga gacagcggtg aaagacctgg gtaaagcggt gtcctataag gggatgtatg
    15901 gcgatgtggc catcgtcgtg tattccggac agtacgtgga aaacggcgtc aaaaagaact
    15961 tcctgccgga caacacgatg gtgctgggga acactcaggc acgcggtctg cgcacctatg
    16021 gctgcattca ggatgcggac gcacagctcg aaggcattaa cgcctctgcc cgttacccga
    16081 aaaactgggt gaccaccggc gatccggcgc gtgagttcac catgattcag tcagcaccgc
    16141 tgatgctgct ggctgaccct gatgagttcg tgtccgtaca actggcgtaa tcatggccct
    16201 tcggggccat tgtttctctg tggaggagtc catgacgaaa gatgaactga ttgcccgtct
    16261 ccgctcgctg ggtgaacaac tgaaccgtga tgtcagcctg acggggacga aagaagaact
    16321 ggcgctccgt gtggcagagc tggaagagga gcttgatgac acggatgaaa ctgccggtca
    16381 ggacacccct ctcagccggg aaaatgtgct gaccggacat gaaaatgagg tgggatcagc
    16441 gcagccggat accgtgattc tggatacgtc tgaactggtc acggtcgtgg cactggtgac
    16501 gctgcatact gatgcacttc acgccacgcg ggatgaacct gtggcatttg tgcttccggg
    16561 aacggcgttc cgtgtctctg ccggtgtggc agccgaaatg acagaacgtg gcctggccag
    16621 aatgcaataa cgggaggcgc tgtggctgat ttcgataacc tgttcgatgc tgccattgtc
    16681 cgcgccgatg aaacgatacg cgggtacatg ggaacgtcag ccaccatgac atccggtgag
    16741 cagtccggcg cagtaatacg tggtgttttt gatgaccctg aaaatatcag ctatgccgga
    16801 cagggcgtgc gcgttgaagg ctccagcccg tccctgtttg tccggactga tgatgtgcgg
    16861 cagctgcggc gcggcgacac gctgaccatc ggtgaggaaa acttctggat agaccggatt
    16921 tcgacggatg atggcggaag ctgtcatctc tggcttgggc gtggcgtgcc gcctgccgtt
    16981 aaccgtcgtc gctgaagggg ggatgtatgg ccataaaagg tcttgagcag gccgttgaaa
    17041 acctcagccg tatcagcaga acggcggtgc ccggtgccgc cgcaatggcc attaaccgcg
    17101 ttgcttcatc cgcgatatcg cagtcggcgt cacaggttgc ccgtgagaca aaggtacgcc
    17161 ggaaactggt aaaggaaagg gccaggctga aaagggccac ggtcaaaaat ccgcaggcca
    17221 gaatcaaggt taaccggggg gatttgcccg taatcaggct gggtaacgcg cgggttgtcc
    17281 tgtcccgacg caggcgtcgt aaaaaggggc agcgttcatc cctgaaaggt ggcgacagcg
    17341 tgcttgtggt gggaaaccgt cgtattcccg gcgcgtttat tcagcaactg aaaaatggcc
    17401 gatggcatgt catgcagcgt gtggccggga aaaaccgtta ccccattgat gtggtgaaaa
    17461 tcccgatggc agtgccgctg accacggcgt ttaaacagaa tattgagcgg atacggcgtg
    17521 aacgtcttcc gaaagagctg ggctatgcgc tgcagcatca actgaggatg gtaataaagc
    17581 gatgaaacat acagaactcc gtgcagccgt actggatgca ctggaaaaac atgacaccgg
    17641 ggcgacgctt tttgatggtc gccccgctgt ttttgatgag gaggattttc cggcagttgc
    17701 cgtttatctc accggcgctg aatacacggg cgaagagctg gacagcgata cctggcaggc
    17761 ggagctgcat attgaagttt tcctgcctgc tcaggtgccg gattcagagc tggattcgtg
    17821 gatggagtcc cggatttatc cggtgatgag cgatgtcccg gcactgtcag atttgatcac
    17881 cagtatggtg gccagtggct atgactaccg gcgcgacgat gatgcgggcc tgtggagttc
    17941 agccgatctg acttatgtca ttacctatga aatgtgagga cgatatgcct gtaccaaatc
    18001 cagtaatgcc ggtgaaaggg gccgggacca ccctgtgggt ttataagggg aacggtgacc
    18061 cttatgcgaa cccgctttca gacgttgact ggtcgcgtct ggctaaagtt aaagacctga
    18121 cgcccggcga actgagcgct gagtcctatg acgacagcta tctcgatgat gaagatgcgg
    18181 actggactgc gaccgggcag gggcagaaat ctgccggaga taccagcttc acgctggcgt
    18241 ggatgcccgg agagcagggg cagcaggcgc tgctggcgtg gtttaatgaa ggggataccc
    18301 gtgcctataa aatccgcttc ccgaacggca cggtcgatgt gttccgcggc tgggtcagca
    18361 gtatcggtaa ggcggtgacg gcgaaggaag tgatcacccg cacggtgaaa gtcaccaacg
    18421 tgggacgtcc gtcgatggca gaagatcgca gcacggtgac ggcgacaacc ggcatgacgg
    18481 tgacacccgc cagtgcttcc gtagtgaaag ggcagagcac tacgctgacc gtggcattcc
    18541 agccggaggg cgcaaccgac aagagcttcc gtgcggtgtc tgcggataaa acaaaagcca
    18601 ccgtgtcggt cagcggtatg accatcaccg tgaacggcgt tgctgcaggc aaggtcaaca
    18661 ttccggttgt atccggtaat ggtgagtttg ctgcggttgc agaaatcacc gtcaccgcca
    18721 gttaatccgg agagtcagcg atgttcctga aaaccgaatc atttgaatat aacggcgtga
    18781 ccgtcacgct ttctgaactg tcagccctgc agcgtattga gcatcttgcc ctgatgaaac
    18841 ggcaggcaga accggcggga tccgacagca accggcaggt tactgtggaa gacgtcatca
    18901 gaaccggcgc gtttctggtg gcgatgtccc tgtggcataa ccatccgaag aagacgcaga
    18961 tgccgtccat gaatgaagcc gttaaacaga ttgagcagga agtgcttacc acctggccca
    19021 cagaggcaat ttctcatgct gaaaacgtgg tgtaccggct gtccggtatg tatgagtttg
    19081 tggtgaataa tgcccccgaa caggcagagg acgccgggcc tgcagagcct gtttctgcgg
    19141 gaaagtgttc gacggtgagc tgagttttgc cctgaaactg gcgcgtgaga tggggcgacc
    19201 cgactggcgt gccatgcttg ccgggatgtc atccacggag tatgccgact ggcaccgctt
    19261 ttacagtacc cattattttc atgatgttct gctggatatg cacttttccg ggctgacgta
    19321 caccgtgctc agcctgtttt tcagcgatcc ggatatgcat ccgctggatt tcagtctgct
    19381 gaaccggcgc gaggctgacg aagagcctga agatgatgtg ctgatgcaga aagcggcagg
    19441 gcttgccgga ggtgtccgct ttggcccgga cgggaatgaa gttatccccg cttccccgga
    19501 tgtggcggac atgacggagg atgacgtaat gctgatgaca gtatcagaag ggatcgcagg
    19561 aggagtccgg tatggctgaa ccggtaggcg atctggtcgt tgatttgagt ctggatgcgg
    19621 ccagatttga cgagcagatg gccagagtca ggcgtcattt ttccggtacg gaaagtgatg
    19681 cgaaaaaaac agcggcagtc gttgaacagt cgctgagccg acaggcgctg gctgcacaga
    19741 aagcggggat ttccgtcggg cagtataaag ccgccatgcg tatgctgcct gcacagttca
    19801 ccgacgtggc cacgcagctt gcaggcgggc aaagtccgtg gctgatcctg ctgcaacagg
    19861 gggggcaggt gaaggactcc ttcggcggga tgatccccat gttcaggggg cttgccggtg
    19921 cgatcaccct gccgatggtg ggggccacct cgctggcggt ggcgaccggt gcgctggcgt
    19981 atgcctggta tcagggcaac tcaaccctgt ccgatttcaa caaaacgctg gtcctttccg
    20041 gcaatcaggc gggtctgacg gcagatcgta tgctggtcct gtccagagcc gggcaggcgg
    20101 cagggctgac gtttaaccag accagcgagt cactcagcgc actggttaag gcgggggtaa
    20161 gcggtgaggc tcagattgcg tccatcagcc agagtgtggc gcgtttctcc tctgcatccg
    20221 gcgtggaggt ggacaaggtc gctgaagcct tcgggaagct gaccacagac ccgacgtcgg
    20281 ggctgacggc gatggcacgc cagttccata acgtgacggc ggagcagatt gcgtatgttg
    20341 ctcagttgca gcgttccggc gatgaagccg gggcattgca ggcggcgaac gaggccgcaa
    20401 cgaaagggtt tgatgaccag acccgccgcc tgaaagagaa catgggcacg ctggagacct
    20461 gggcagacag gactgcgcgg gcattcaaat ccatgtggga tgcggtgctg gatattggtc
    20521 gtcctgatac cgcgcaggag atgctgatta aggcagaggc tgcgtttaag aaagcagacg
    20581 acatctggaa tctgcgcaag gatgattatt ttgttaacga tgaagcgcgg gcgcgttact
    20641 gggatgatcg tgaaaaggcc cgtcttgcgc ttgaagccgc ccgaaagaag gctgaacagc
    20701 agagtcaaca ggacaaaaat gcgcagcagc agagcgatac cgaagcgtca cggctgaaat
    20761 ataccgaaga ggcgcagaag gcttacgaac ggctgcagac gccgctggag aaatatgccg
    20821 cccgtcagga agaactgaat aaggccctga aagacggaaa aatcctgcag gcagattaca
    20881 acacgctgat ggcggcagcg aaaaaggact atgaaacgac actgaaaaag ccgaagcagt
    20941 ccggcgtgaa ggtgtctgcg ggcgatcgtc aggaagacag tgctcatgct gccctgctga
    21001 cgcttcaggc tgaactccgg acgctggaga agcatgccgg agcaaatgag aaaatcagcc
    21061 agcagcgccg ggatttgtgg aaggcggaga gtcagttcgc ggtactggag gaggcggcgc
    21121 aacgtcgcca gctgtctgta caggagaaat ccctgctggc gcataaagat gagacgctgg
    21181 agtacaaacg ccagctggct gcacttggcg acaaggttac gtatcaggag cgcctgaacg
    21241 cgctggcgca gcaggcggat aaattcgcac agcagcaacg ggcaaaacgg gccgccattg
    21301 atgcgaaaag ccgggggctg actgaccggc aggcagaacg ggaagccacg gaacagcgcc
    21361 tgaaggaaca gtatggcgat aatccgctgg cgctgaataa cgtcatgtca gagcagaaaa
    21421 agacctgggc ggctgaagac cagcttcgcg ggaactggat ggcaggcctg aagtccggct
    21481 ggagtgagtg ggaagagagc gccacggaca gtatgtcgca ggtaaaaagt gcagccacgc
    21541 agacctttga tggtattgca cagaatatgg cggcgatgct gaccggcagt gagcagaact
    21601 ggcgcagctt cacccgttcc gtgctgtcca tgatgacaga aattctgctt aagcaggcaa
    21661 tggtggggat tgtcgggagt atcggcagcg ccattggcgg ggctgttggt ggcggcgcat
    21721 ccgcgtcagg cggtacagcc attcaggccg ctgcggcgaa attccatttt gcaaccggag
    21781 gatttacggg aaccggcggc aaatatgagc cagcggggat tgttcaccgt ggtgagtttg
    21841 tcttcacgaa ggaggcaacc agccggattg gcgtggggaa tctttaccgg ctgatgcgcg
    21901 gctatgccac cggcggttat gtcggtacac cgggcagcat ggcagacagc cggtcgcagg
    21961 cgtccgggac gtttgagcag aataaccatg tggtgattaa caacgacggc acgaacgggc
    22021 agataggtcc ggctgctctg aaggcggtgt atgacatggc ccgcaagggt gcccgtgatg
    22081 aaattcagac acagatgcgt gatggtggcc tgttctccgg aggtggacga tgaagacctt
    22141 ccgctggaaa gtgaaacccg gtatggatgt ggcttcggtc ccttctgtaa gaaaggtgcg
    22201 ctttggtgat ggctattctc agcgagcgcc tgccgggctg aatgccaacc tgaaaacgta
    22261 cagcgtgacg ctttctgtcc cccgtgagga ggccacggta ctggagtcgt ttctggaaga
    22321 gcacgggggc tggaaatcct ttctgtggac gccgccttat gagtggcagc agataaaggt
    22381 gacctgcgca aaatggtcgt cgcgggtcag tatgctgcgt gttgagttca gcgcagagtt
    22441 tgaacaggtg gtgaactgat gcaggatatc cggcaggaaa cactgaatga atgcacccgt
    22501 gcggagcagt cggccagcgt ggtgctctgg gaaatcgacc tgacagaggt cggtggagaa
    22561 cgttattttt tctgtaatga gcagaacgaa aaaggtgagc cggtcacctg gcaggggcga
    22621 cagtatcagc cgtatcccat tcaggggagc ggttttgaac tgaatggcaa aggcaccagt
    22681 acgcgcccca cgctgacggt ttctaacctg tacggtatgg tcaccgggat ggcggaagat
    22741 ctgcagagtc tggtcggcgg aacggtggtc cggcgtaagg tttacgcccg ttttctggat
    22801 gcggtgaact tcgtcaacgg aaacagttat gccgatccgg agcaggaggt gatcagccgc
    22861 tggcgcattg agcagtgcag cgaactgagc gcggtgagtg cctcctttgt actgtccacg
    22921 ccgacggaaa cggatggcgc tgtttttccg ggacgtatca tgctggccaa cacctgcacc
    22981 tggacctatc gcggtgacga gtgcggttat agcggtccgg ctgtcgcgga tgaatatgac
    23041 cagccaacgt ccgatatcac gaaggataaa tgcagcaaat gcctgagcgg ttgtaagttc
    23101 cgcaataacg tcggcaactt tggcggcttc ctttccatta acaaactttc gcagtaatcc
    23161 catgacacag acagaatcag cgattctggc gcacgcccgg cgatgtgcgc cagcggagtc
    23221 gtgtggcttc gtggtgagaa cgccgaaggg ggaaagatat tttccctgcg tgaatatctc
    23281 cggtgagccg gaggcgtatt tccggatgtc gccggaggac tggctgcggg cagagatgca
    23341 gggtgagatt gtggcgctgg tccacagcca ccccggtggt ctgccctggc tgagtgaggc
    23401 tgacaggcgg ctgcaggtgc agagcgattt gccgtggtgg ctggtctgcc ggggtgagat
    23461 tcataaattc cgctgtgtgc cgcatcttac cgggcggtgc tttgagcacg gggtgacgga
    23521 ctgttacacg ctgttccggg atgcttatca tctggcgggg attgagatgc cggattttca
    23581 tcgcggggat gactggtggc gtcacggtca gaatctctat ctggataatc tggaggccac
    23641 agggctgtat caggtgccgt tgtcatcagc acaaccgggc gatgtgctgc tgtgctgttt
    23701 tggttcatcg gtgccgaatc atgccgccat ttactgtggt gatggcgagc tgctgcacca
    23761 tattcctgaa caactgagca aacgagagag gtataccgac aaatggcagc gacgcacaca
    23821 ctccctctgg cgtcaccggg catggcgcgc atctgccttt acggggattt acaacgattt
    23881 ggccgccgca tcgaccttcg tgtgaaaacg ggggctgaag ccatccgggc gctggccaca
    23941 cagctcccgg cgtttcgtca gaaactgagc gacggctggt atcaggtacg gattgccggg
    24001 cgtgatacag gtgaaacgga attatcagcc cgtcttaatg aaccgctggc aaatggtgct
    24061 gtgatccata tcgtgccgcg tctggcagga gccaaaagtg gcggtgtgtt tcaggctgtg
    24121 ctgggggcgg ctgttatggc ggttgctata tggatgccgg gggtaggaat tatggcgagt
    24181 aatctgctgt tttctctcgg tgccagtatg acgcttggcg gtgttgcaca gatgctggcc
    24241 cctaaaccca gaaccccccg cacacagaca acggataacg gcaaacagaa cacctatttt
    24301 tcttcactgg ataatatggt tgcccagggc aatgttctgc ccgttctgta cggtgaaatg
    24361 cgcgtgggat cacgtgtggt atctcaggag atcagcacag cagatgaagg tgatggtggt
    24421 caggttgtgg tgattggtcg ctgatgaaaa acgttttatg tgaaaccgcc tccgggcggt
    24481 tttatcgttt atggagcatg acgaatgggt aaaggcagca gtaaggggca taccccgcgc
    24541 gaagcgaagg acaacctgaa gtccacgcag ctgctgagtg tgatcgatgc catcagcgaa
    24601 gggccggttg aaggtccggt ggatggatta aaaagcgtgc tgctgaacag tacgccggtg
    24661 ctggacagtg aggggaatac caatatatcc ggcgtcacgg tggtgttccg ggccggtgag
    24721 caggagcagt caccgccgga gggatttgaa tcctccggct ccgagacggt gctgggtacg
    24781 gaagtgaaat atgacacgcc gatcacccgg accatcacgt cggcaaacat cgaccgtctg
    24841 cgctttacct tcggtgtaca ggcactggtg gaaaccacct caaagggaga caggaatccg
    24901 tcggaagttc gcctgctggt tcagatacag cgtaacggtg gctgggtgac ggaaaaagac
    24961 atcaccatta agggcaaaac cacctcgcag tatctggcct cggtggtggt ggataacctg
    25021 ccgccgcgcc cgtttaatat ccggatgcgc aggatgacgc cggacagcac cacagaccag
    25081 ctgctgaaca aaacgctctg gtcgtcatac accgaaatca tcgatgtgaa acagtgctac
    25141 ccgaacacgg cactggttgg cgtgcaggtg gactcggagc agttcggcag ccagcaggtg
    25201 agccgtaatt atcatcttcg cgggcgcatt ctgcaggtgc cgtcgaacta taacccgcag
    25261 acgcggcaat acagcggtat ctgggacgga acgttaaaac cggcatacag caacaacatg
    25321 gcctggtgtc tgtgggatat gctgacccat ccgcgctacg gcatggggaa acgtcttggt
    25381 gcggcggatg tggataaatg ggcgctgtat gtcatcggcc agtactgcga ccagtcggtg
    25441 ccggacggct ttggcggcac ggagccgcgc attacctgta atgcgtacct gaccacacag
    25501 cgcaaggcgt gggatgtgct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg
    25561 aacgggcaga cgctgacgtt cgtgcaggac cgaccatcag ataaggtgtg gacctataac
    25621 cgcagtaatg tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg
    25681 aaggaccgcc ataatgccgt tgaggtgaac tggattgacc cggataacgg ctgggagacg
    25741 gcgacagagc tcgtggagga tacgcaggcc attgcccgtt acggccgtaa cgtcacgaag
    25801 atggatgcct ttggctgtac cagccggggg caggcacacc gcgccgggct gtggctgatt
    25861 aaaacggagc tgctggaaac gcagaccgtg gacttcagcg tgggtgctga agggcttcgc
    25921 catgtaccgg gggatgtcat tgaaatctgc gatgatgact atgccggtat cagcaccggt
    25981 ggtcgtgtgc tggcggtgaa cagccagacc cggacgctga cgctcgaccg tgaaatcacg
    26041 ctgccatcct ccggtaccac gctgataagc ctggttgacg gaagtggcaa tccggtcagc
    26101 gtggaggttc agtccgtcac cgacggcgtg aaggtgaaag tgagccgtgt tcctgacggt
    26161 gttgctgaat acagcgtatg ggggctgaag ctgccgacgc tgcgccagcg actgttccgc
    26221 tgcgtgagta tccgtgaaaa cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg
    26281 ccggaaaaag agaccatcgt ggataacggg gcgcactttg acggcgaaca gagtggcacg
    26341 gtgaatggtg tcacgccgcc cgcggtgcag cacctgaccg cagaagtcac cgcagacagc
    26401 ggggaatatc aggtgctggc gcgatgggac acaccgaagg tggtgaaggg ggtgagcttt
    26461 atgcttcgcc tgaccgtggc agcggatgac ggcagtgagc ggctggtcag cacggccagg
    26521 acgacggaaa ccacataccg cttcaggcaa ctggcgctgg ggaactacag gctgacagtc
    26581 cgggcggtaa atgcgtgggg gcagcagggg gatccggcgt cggtatcgtt ccggattgcc
    26641 gcaccggcag cgccatcgcg gattgagctg acgccgggct attttcagat aaccgcaacg
    26701 ccgcatcttg ccgtttatga tccgacggta cagtttgagt tctggttctc ggaaaagcgg
    26761 attgcggata tcaggcaggt tgaagccagc gcgcgttatc ttggtacggc gctgtactgg
    26821 atagccgcca gtatcaatat caaaccgggc catgattatt atttttacat tcgcagtgtg
    26881 aacaccgttg gcaaatcggc atttgtggag gctgttggcc agccgagtga tgatgcatcc
    26941 ggctatctgg attttttcaa aggagagata gggaaaaccc atctggctca ggagttgtgg
    27001 acgcagattg ataacggtca gcttgcgcct gacctggctg aaatcaggac gtccattacg
    27061 gatgtcagca atgaaatcac gcagaccgtc aataagaaac tggaagacca gagtgcagcg
    27121 atccagcaga tacagaaggt tcaggttgat acaaataata acctgaacag catgtgggct
    27181 gtgaagctgc agcagatgca ggacggacgc ctttatatcg cgggtattgg tgccggtatt
    27241 gagaataccc ctgacggtat gcagagtcag gtgctgctgg cggcggacag gattgcgatg
    27301 attaatcctg cgaatggcaa tacaaagccg atgtttgttg ggcagggcga tcagatattc
    27361 atgaacgaag tgttcctgaa atatctgacg gctcccacca ttaccagcgg tggcaatccc
    27421 ccaacgtttt cactgacacc tgatggtcga ctttctgcga gaaatgcgga tatcagcggt
    27481 aacgtgaatg cgaactccgg gacgctcaat aatgtcacga ttaaccagaa ctgtcggatt
    27541 ctgggaaaac tgtcagctaa ccagattgaa ggtgatattg tcaaaacggt gggaaaagcc
    27601 tttccgagaa atggcagtta tgccagcggt acaataacgg tcactgtgta cgatgaccag
    27661 gcttttgacc gtcagatagt cgtccccccc gttctgtttc gcggtggtaa gcatgaaaac
    27721 ttcaacagca acaaccaaca gtcatactgg tattcaacct gtaagctgca ggtgctgaag
    27781 aacggacagg aaatctttca gcaacccgcg acggatgtca gcagggtatt ttcatcagtc
    27841 attgatatgc ctgccggaca cggccatgtc accctgactt tcaatgtttc ttcatatggt
    27901 gcaaataact ggacgccaac gaccagtatc agcgaccttc ttgttgtcgt gatgaagaaa
    27961 tcaacagccg gcatcagtat cagttgaatt ttaaaaccca aattcgggca ccagaaatgg
    28021 tgcctttttt atttgtggag tgaatatggc agtacagatt tcaggtgtgc tgaaagacgg
    28081 tgcaggaaaa ccgatacaga actgcaccat tcagctcaaa gcaaaacgta acagcaccac
    28141 agttgtggtg aacacggtgg cctcagaaaa tccggatgaa gccggacgtt acagcatgga
    28201 tgttgagtat ggccagtaca gcgtcgccct gctggttgaa ggttttccgc cttcacatgc
    28261 cgggaccatc accgtgtatg aggattctaa gccggggaca ttgaatgatt ttctgggcgc
    28321 tgcaacagaa gatgatgttt gtccggaggc actgtatcgt tttgaaaaga tggtggaaga
    28381 ggcggcacgc aacgctgaag ccgcctctca gagcgcagcg gcagcaaaga aatcagaaac
    28441 agcagcggca tcgtccagga acgcggcgaa aacatcagag acgaatgcag gtaacagcgc
    28501 gaaagcggca gcttcttcaa aaacagccgc acaaaacgca gcaacagcgg cagaacgttc
    28561 agagacaaat gcccgtgcgt cagaagaagc ctccgcagac agtgaagagg cttcccgccg
    28621 taatgcagag tcagccgctg aaaatgccgg agtcgccacc acaaaagcgc gggaggccgc
    28681 agcagacgca acaaaggccg ggcagaaaaa ggatgaggct ctgtcggcag cgacacgagc
    28741 tgaaaaggcg gcagaccgcg cagaagccgc agcggaagtg actgcagagc cctgtgcgaa
    28801 tatagtgccg ccgctgcctg atgtgtggat accgtttaac gattcactgg atatgattac
    28861 gggtttttcg ccatcttata aaaagatagt tattggtgac gatgaaataa caatgccagg
    28921 cgacaagatt gttaagttta aacgtgcttc aacagcaacg tatattaata agtccggcca
    28981 actcaagctt gctgaagttg acgaaccgcg atttgagcgc gatggcttat tgattgaagg
    29041 acagaggaca aattatctga ggaactcaaa taaaccagac tcatggactg ttcattccgc
    29101 actgaataaa acatttggca ctgataaaca ggggttcaat tatgccacgg tgacacccac
    29161 ggaaagtata gtgggaacaa caggtggcta tactgtgcat ggtgtggttg cagcagacag
    29221 attcccgctg gcaagtggtg aatgtttcac tttttcgtgc cgggttaaag gcgctaaagc
    29281 acgatgcagg ttaagagttt cagttattat tggtggaaca gataccttct ctgctgactc
    29341 ttatcttgat ctggataccc ggatcgcaac agtaagcggt aatacatccc ttataacagc
    29401 caaagctgaa caacagggcg agtggaccta ctatgaggcc acttatacag ctaatacgga
    29461 cattgatacc gttaactgtg ctttttatat gacaaataaa ataagtaatg agacattcta
    29521 tgatgactca acattaacca tgacgacgcc gcaaattgaa ctgggcaata cggcatcgtc
    29581 atttattgta actacaatgc caacaacacg cgcaagtgat gtagttacta tcccatcgca
    29641 gaataacctg tcaacacggc cttttacggt attgtgcgaa gtaagcagga actggagtac
    29701 accgcccaat gttgcgccaa ggatatttga tgttggaggg cacagtattg atgataatta
    29761 tttatcgctg gggtttgttg caacaggaaa gataagcgcc aacgtaggaa tggttcagcc
    29821 acaaattgcc tcagatggag aaaggttcat tgtgggtgtg agagctaaat ctgatttatc
    29881 agtaaatgca atatgcaatg gtaattatac aacaaacctt aatggtaaaa tatttggagt
    29941 tacagcaaca tcgtaccggt ttggtgggca gaccgcagca ggaacgcgtc atttgtttgg
    30001 acacatcaga aatttcagag tctggtttaa agaattaaat gacaggcaaa tcaaggaggc
    30061 agtatgaaag atttaacttt gaaatttcct ggtaacagag agtttaaatc cttcctgtca
    30121 tctcttgact gggaggagga tgaagacctc cagaataaac tgttagtcga tgaaattggt
    30181 ttcacctaca cagaaacagg ggtaacagaa gagggagaac ctgtctgtgt ccggaatgac
    30241 ggttattttg tcaacattcg cattcttgat gacttgtttg atgtttctgt attctctgat
    30301 tatgtcgtgg agctggaaac accgcttcgg gaatggagct gaaaggagga aataatggat
    30361 ataagcccct tacttcatgc actttgtgct gtggctgcgc agatactggt tggtcttttt
    30421 accggaaact gggcttacgg agcgatagcc ggttgtacgt tcttcattgc gcgtgaacat
    30481 acccaggcag aatatcgctg gattgaaatg ttcgggcatg gcaagcgaat gaatatgccg
    30541 tggtggggcg gttttgatcc gcgagcttgg gatgtggcaa gcctgatgga ttttgctgtg
    30601 ccggtggtgg cgtgtctgct ggtctggctg ttggttaatc gtgggtgaaa aaaggtgagc
    30661 tgtatatgca acggaggaag aaaccccgtt gctggaagcc aggaaaaagt atcgggtgtt
    30721 gctgtcctcg gatgttgtgc taattgggtg ataacagatc ctgtgtatgg gagtttccct
    30781 ttttgcgtgc gttgatgctg gtaacggcaa acgtcatgat gaacaaaacc gcgaataaga
    30841 cgacaatcgt cggagctggt gcgctgtcga taaaaaagga caaccagaca ccagtcattg
    30901 acactattac cgatatgcca gtcgccagta ggagcgcaat atgaaagcgt tgtgtcagga
    30961 gtaccgcaat ggcgccaggc gcaatgagca gagaaatgga gagaatgata cctaccgctt
    31021 tcagcgtcgc cacaatggtc agagagacca tgcacagcag cccataatgc agccatctcg
    31081 tatgcaaacc gcttacctgc gcctgttgat aatcaaagct gaacagcaaa aaatcccgcc
    31141 attttacgct aataacaagt gtaaccagcc cggcaataat catcgtctgg attatatcgc
    31201 cgatggttat cccgagcatg tcgccgaaaa gaatatggtc aagatgtacg tctggtttga
    31261 ctgcaatata caagatcaga cctgcggcaa acatgccaga aaaaacaatc cccatcactg
    31321 tatcttgctt gattcggctg ttgtctttca ggtatcccgt cgccaccgca cagaatactc
    31381 cggcgacgaa tgcccctgta gccaaaggta accccagaat ccaggcaagc acgatgccag
    31441 gaaaaaccgc atgactcatc gcgtcaccca tcagtgccca acctttcagg accagaaaga
    31501 ccgacagcag cgcacagggt atggaaacta ccagtgaaat cagcagtgcg tgactcataa
    31561 aggtgaactg cagtggctcc agtaataatg ccatcatgag cgatcctcca gcgccttatg
    31621 cgcacgccga cgatttgcca aaagcccatg ggtgggcgca aagacaaatg ctaaaagaaa
    31681 cagcagtgtc tgcgccacca cgatgattcc acctgtcgcg ccgtccagaa agtaactgac
    31741 ccacgcgccg aggaaactgg ttacactgcc aatagttaca gcaatcatga gcaggcgggg
    31801 aaaacgatcg gttaacaacc atgctgttgc accgggagtc accaccagac agatcaccag
    31861 aaaggcacca accgtttgca gcgccgcaac ggtcgaaacc gacagcaggg tgaagaagag
    31921 gagttttaat ctgcccggat gcaatccaat agcacgggcg tgattctcgt cgaaaaaggt
    31981 caccataaga tctttccatt tgaaaaaaag cacgatgatc gagagaatgc caatgatagt
    32041 gagttgcagg atatccgccg gatcgattgc cagaatattg cccaggacga tggtctgaat
    32101 gttcacagat gtcgggttca atgacaccat aaacagcccc aggccaaaaa aggacgagaa
    32161 gatcagaccg atgatggcat cttcttttaa gcgggtgcgt tggttaagaa acagcatgct
    32221 gcccgcagcc agtccgccgg aaaagaaggc tcccagagaa aacggaagcc ccagcatata
    32281 tgcccccgca acacccggga caatagagtg cgacaacgca tcgccaatca gcgaccagcc
    32341 ttttagcatc agatagcatg acaggaaagc gcagagaccg ccgaccatcg ccgagaccca
    32401 catcgcattg agcatatatt catagctgaa gggttccagt agcacgttca tctttcttcc
    32461 ctctgcaccg ctgacggtcg atgagccaca aaagggcgtt catcgtcagt aataatgctt
    32521 tcttccgagc cgttaagtgt gacatggcgc agtacgccgc taaaagccag ctctaggttc
    32581 tcggctgtaa aagtggtgtc tgtcggcccg cttgccagga cggtgccttt gaccatgacc
    32641 gtataatcgc aaaatgtcgt gacggatccg agattgtgtg ttgagacaag catcgttttg
    32701 ccttccgcgc gtaactccct caacaggctg atgattttgg cttcggtttt cacatcaacg
    32761 ccagtaaaag gttcatccag caggatcaca tcaccctgct gtgcaatcgc tctcgccaga
    32821 aagacgcgtt tcttttgtcc accggaaagc tcgccgattt gtcgatggcg aaaattcacc
    32881 atatcgacac gttccagcgc atcggtgaca atttgacgat ccctcttttt ggcgatacgc
    32941 aacataccca tatgcccata gcggcccatc atcaccacat cttccaccag tacaggaaat
    33001 gaccagtcaa catcttctga ctgagggacg taggcaacca ggtttttctg taacgcctgt
    33061 cgcgtgggaa tacccagaac agatatcttt ccgctggtca gacgcacaaa tcccataatc
    33121 gctttaaaca acgtcgattt tccggaaccg tttaccccaa ccagagcggc aatagatcca
    33181 cttggtactg tgaaggatgc atcgcgcagt gctgtgtgcc cgttacgcca ggtgacagta
    33241 acatcattaa cgacaatgcc tgcagattgc atcattattt ctcccttttc ccagctttaa
    33301 ttccctgtac cagcgtactg gtagtaacct taagaaggtc gatatacgtc ggtaccgggc
    33361 cgttttctgt gctcagggaa tcgacataga gcacaccgcc gtagtgtgcg ccggtttcac
    33421 gcgcaacctg acgcgctggt ttatcggaaa tcgtactctc gctaaagact gccgggatat
    33481 gatttttctt aactatatca acaaccttac gtacctgctg cggtgttcct tgttgatcgg
    33541 cattaatcgg ccacagataa agctctttta gccccaaatc gcgtgcgaga taagaaaaag
    33601 ccccttcact ggtgaccatc catcgctgat tctcagggag ttccgtaatc tgcttacgca
    33661 ggggggcaag ggtttgggta atcttggctt tataagtatc ggcattgcgt tggtaggttt
    33721 gtgcatttgc cggatcgtat tttatcaacg catcacgaat attatcgacg taaatcagag
    33781 cattatctgg cgacatccat gcatgggggt taggtttgcc ctcatagggt ccttcggtga
    33841 tccctacagg cgtcacaccc gaagagacaa ttacttctgg aaccccattg agatgctggt
    33901 aaaagcgttg gaaccacaat tccagattca taccattggc gagaatcagt tgtgccccct
    33961 gcgcacgttt aatatcgcca ggggtaggct gatactcatg aatttctgca ccaggcttgg
    34021 ttatggatga gacttctgca gcatctcccg ccacgttttt ggccatatcc gcgatgatgg
    34081 taaatgttgt aatgaccttg aatttttcag ttgcacttgc ctgaaatgcg atcgagcagg
    34141 tgagtgcgag cccccccaag agcatggtta ctttttttat cgagtgcata atttagtacc
    34201 tatagtgagc aaaattagaa caaatatagc aacggctatg ttttatagca cagcctatac
    34261 aaaatgaaaa tgattatcat tagcagcttt gaggcccaaa acagggatta ggttgtttcc
    34321 tgctggcgac gcgttattga ggaaaaaagt aaggcagcta gtaggctttg aaataacgtt
    34381 ttgcggttgg ctgatgcaat ttcgagagtg catgtattga atgatttcta gccgttactg
    34441 attttcgtat tttttgtatg ggaggatttg cacctccccc caccgagcct ccatgacttt
    34501 actccactgt ctctagggct gctgtgtgcc aggagcggaa gcaggggggc cagagactcc
    34561 ttaaaagttg atagctcgtt ttgcagggag acttacacgg taagcgttat tccgaagagc
    34621 ttaaaattga agcagtcaaa cgggttgttg agcgtgttta tgcgctgcca tctgattaat
    34681 cgacggaagc agttccgttt ggtctatgat gcagttgtcc ggcaggcgct ctgtgacgca
    34741 aaagaacgat aggagtagca ttgaagtgaa cgaggttatg cttgtcttat attatcctct
    34801 attttaaaaa catcagctgg ttgtgtaaga gtttttattg ttttatcttg ttgactttgc
    34861 ttt
//
LOCUS       DACADF010000036        34405 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.036, whole genome shotgun sequence.
ACCESSION   DACADF010000036 DACADF010000000
VERSION     DACADF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.036"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..726
                     /locus_tag="ISB30_001663"
     CDS             <1..726
                     /locus_tag="ISB30_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAP1989304.1"
                     /translation="KYTAALCFATCVAFSSSAIADIVISGTRVIYKSDQKSVNIRLEN
                     KGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASSVLPKD
                     RESVFWFNVLEVPPKPDAATVANQSLLQLAFRTRIKLFYRPEALKGNPSEAPLALKWS
                     WSGSEGKAALRVANPTPYYVSFSSGDLEASGKRFPIDVKMIAPFSDEVMKVTGLNGKA
                     NSAKVHFYAINDFGGAIEGNASL"
     gene            761..3361
                     /gene="htrE"
                     /locus_tag="ISB30_001664"
     CDS             761..3361
                     /gene="htrE"
                     /locus_tag="ISB30_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HAP1989305.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNGAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGVYDVSVYVNDQPVINQSITFVAIEGKKNAQACITLKNLL
                     QFHINSPDINSEKAVLLARDEALGNCLNLTEIIPQASVRYDVNDQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRKNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGGNYDFKNRYIQRDIASLRSQFIIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     IEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSISAQRSWN
                     EDGDADDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDDQGNVIGNVGQGGQAFVRGIEQQGNINIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            3378..3950
                     /locus_tag="ISB30_001665"
     CDS             3378..3950
                     /locus_tag="ISB30_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1989306.1"
                     /translation="MIKTTPHKIAILMGVLLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKITNKTTESQADFKLVASGCSSGISWIDTTLTGNAL
                     SSSSKLIIPLSGDLSSTTSNIGMGFKKRTTDDATFLQPNNSADKIRWSTEEMQPDKGL
                     EMTVALRETAAGQGVPGIFRALATFNFIYQ"
     gene            3965..4567
                     /gene="yadL"
                     /locus_tag="ISB30_001666"
     CDS             3965..4567
                     /gene="yadL"
                     /locus_tag="ISB30_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HAP1989307.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEMGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTATDKASAVAVEVWSTETPATGRATQFSCVTPASQEVTISTA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4594..5190
                     /locus_tag="ISB30_001667"
     CDS             4594..5190
                     /locus_tag="ISB30_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1989308.1"
                     /translation="MHPTQRKLMKRIILFLSLLFPITCPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSGNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVTTGAKNVGVVLFSIQDPANIFNVLSSAGNSRSIYPVMTS
                     ALNNSSWKFYARMQKIDPALDVISGQVMSHILVDVHYE"
     gene            5242..6495
                     /locus_tag="ISB30_001668"
     CDS             5242..6495
                     /locus_tag="ISB30_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HAP1989309.1"
                     /translation="MKTFFKYFVFLVLSCSCFTAISKDTSFLVGNNAGVGDGNFNGPS
                     TAAQVTFRYASTTNNLVFYKPTQLGPTGVKLRWEQLDTASGGGFLYCNASDRANPDGL
                     MDIENAMADSGKTYGGHKLFKTSVPGLYYTLFIAKLWSAYSTKTTISDSGLYIGDTTP
                     QKFQWIITDPDLQHIGCDNAMRYDRFWAIGGVVHDLTIEFYTDTDFNPTTNQDVSLSS
                     NSTYLYAFKAYSPGSRVVDHSHHLYIDFDLLNVKLTNPTCFTAVLTGKSVSGSTVKMG
                     EYAPGQIKNGATPVPFDISLQNCVRVGDIETKLSSGKLGTENKQLLGNTLTGSGAAKG
                     VAVLIEGLANRKSALMILKPNDSTSVYKDNTGQAQDHDSDAIYPEDQGITYPLHFQAT
                     LKQDGNIAIEPGEFKATSTFQVTYP"
     gene            6608..7402
                     /gene="panB"
                     /locus_tag="ISB30_001669"
     CDS             6608..7402
                     /gene="panB"
                     /locus_tag="ISB30_001669"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP1989310.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAA
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7414..8265
                     /gene="panC"
                     /locus_tag="ISB30_001670"
     CDS             7414..8265
                     /gene="panC"
                     /locus_tag="ISB30_001670"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP1989311.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIIGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8347..8544)
                     /locus_tag="ISB30_001671"
     CDS             complement(8347..8544)
                     /locus_tag="ISB30_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1989312.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYKEGLQKVRLALRLLSKGM
                     SREDVAEMANLPLAEIDKMIN"
     gene            complement(8613..9533)
                     /locus_tag="ISB30_001672"
     CDS             complement(8613..9533)
                     /locus_tag="ISB30_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAP1989313.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGSTGYLHVVIEHQSKPDKKMTFRMMRYAIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNNPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEVRQEVRQEVIQEVRQEVRQEF
                     ALRFLSKGMSREDVAEMANLPLAEVDKLIS"
     gene            9807..10187
                     /gene="panD"
                     /locus_tag="ISB30_001673"
     CDS             9807..10187
                     /gene="panD"
                     /locus_tag="ISB30_001673"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAP1989314.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10191..11420)
                     /locus_tag="ISB30_001674"
     CDS             complement(10191..11420)
                     /locus_tag="ISB30_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP1989315.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGLGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTQVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11484..11924)
                     /locus_tag="ISB30_001675"
     CDS             complement(11484..11924)
                     /locus_tag="ISB30_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP1989316.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12029..12799)
                     /locus_tag="ISB30_001676"
     CDS             complement(12029..12799)
                     /locus_tag="ISB30_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1989317.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12796..13722)
                     /locus_tag="ISB30_001677"
     CDS             complement(12796..13722)
                     /locus_tag="ISB30_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1989318.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            13831..14493
                     /gene="can"
                     /locus_tag="ISB30_001678"
     CDS             13831..14493
                     /gene="can"
                     /locus_tag="ISB30_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAP1989319.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14534..15070)
                     /gene="hpt"
                     /locus_tag="ISB30_001679"
     CDS             complement(14534..15070)
                     /gene="hpt"
                     /locus_tag="ISB30_001679"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP1989320.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15276..17666
                     /locus_tag="ISB30_001680"
     CDS             15276..17666
                     /locus_tag="ISB30_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/quinate/shikimate family membrane-bound
                     PQQ-dependent dehydrogenase"
                     /protein_id="HAP1989321.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLSVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17713..19263)
                     /gene="cueO"
                     /locus_tag="ISB30_001681"
     CDS             complement(17713..19263)
                     /gene="cueO"
                     /locus_tag="ISB30_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAP1989322.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRNLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19429..19776
                     /locus_tag="ISB30_001682"
     CDS             19429..19776
                     /locus_tag="ISB30_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAP1989323.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            19882..20748
                     /gene="speE"
                     /locus_tag="ISB30_001683"
     CDS             19882..20748
                     /gene="speE"
                     /locus_tag="ISB30_001683"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAP1989324.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            20764..21558
                     /gene="speD"
                     /locus_tag="ISB30_001684"
     CDS             20764..21558
                     /gene="speD"
                     /locus_tag="ISB30_001684"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAP1989325.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21596..21958)
                     /locus_tag="ISB30_001685"
     CDS             complement(21596..21958)
                     /locus_tag="ISB30_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAP1989326.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22133..24730)
                     /gene="acnB"
                     /locus_tag="ISB30_001686"
     CDS             complement(22133..24730)
                     /gene="acnB"
                     /locus_tag="ISB30_001686"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAP1989327.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            25085..26848
                     /locus_tag="ISB30_001687"
     CDS             25085..26848
                     /locus_tag="ISB30_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAP1989328.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QTAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHDHHHHDDDDYHHHDGGHRDGNDWQ
                     HNGDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVTYHDQDMAKRFHQTDVNGGM
                     SATQLPALSRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGNYDDFRE
                     FSRHQPLTQQQKDAARQRYQSASPEQRQAAREKMQTSPQNQQQRDAARQRIQSASPEQ
                     RQVFKEKVQQRPLNQEQRDNARQRVQSASPEQRPFFREKVQESRPQRLNDSNRTARLN
                     NEQRSAVRERLSERGARRLER"
     gene            complement(26919..28343)
                     /gene="lpdA"
                     /locus_tag="ISB30_001688"
     CDS             complement(26919..28343)
                     /gene="lpdA"
                     /locus_tag="ISB30_001688"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP1989329.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28551..30443)
                     /gene="aceF"
                     /locus_tag="ISB30_001689"
     CDS             complement(28551..30443)
                     /gene="aceF"
                     /locus_tag="ISB30_001689"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP1989330.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAASAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30458..33121)
                     /gene="aceE"
                     /locus_tag="ISB30_001690"
     CDS             complement(30458..33121)
                     /gene="aceE"
                     /locus_tag="ISB30_001690"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAP1989331.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33282..34046)
                     /gene="pdhR"
                     /locus_tag="ISB30_001691"
     CDS             complement(33282..34046)
                     /gene="pdhR"
                     /locus_tag="ISB30_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAP1989332.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8605 a         8974 c         8256 g         8570 t
ORIGIN      
        1 aaatatacag cagcattatg cttcgcaacc tgtgtagctt ttagctcgtc agctatagca
       61 gatatcgtaa tttccggtac acgcgtaatc tataaaagcg accaaaaaag tgtcaacatt
      121 cgcctggaaa ataaaggcaa taacccatta cttgttcaga gttggctgga tactggtgat
      181 gacaatgctg agcctggcag tattaccgtc ccctttactg cgactccgcc cgtatcacgc
      241 attgatgcaa aacgcggcca aaccatcaaa ctgatgtaca ccgccagcag cgtcctgcca
      301 aaagacagag aaagtgtgtt ctggtttaac gtactagaag ttcctccaaa accagatgcc
      361 gcaacagttg caaatcaaag cctgctgcaa ttggcatttc gcacacgtat aaaactgttc
      421 tatcgcccgg aagcattgaa ggggaatccc tcagaagcac cattagccct gaaatggtcc
      481 tggtcaggtt ccgagggtaa agcagcatta cgagtggcca atccaacccc ttactacgtc
      541 tcttttagca gtggtgattt agaagccagc ggtaaacgct ttccgattga tgtgaaaatg
      601 attgcaccat tcagtgatga agtcatgaaa gtcactggcc ttaatggcaa agcaaattct
      661 gcaaaagtgc atttttacgc cattaatgat tttggtggtg ccattgaagg taatgccagt
      721 ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa aaccaactaa
      781 aaattatcac aatcatctga cccgtatcgc cacgttttgc gcgctgctgt attgcaatgg
      841 tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc agaatgcctc
      901 aaatattgat ctcagccggt atagtgaagg taaccccgct ataccgggtg tgtacgacgt
      961 tagtgtttat gtcaacgatc aacctgttat taaccaaagc attacatttg tcgcaattga
     1021 aggaaaaaag aacgcacaag cctgtatcac acttaaaaat ttattgcagt tccatattaa
     1081 ttctcccgat ataaacagcg aaaaagccgt tctgcttgcc cgggatgaag ctctcggcaa
     1141 ttgcctcaat ttgacggaaa ttatccctca ggcttccgtt cgttatgacg ttaacgatca
     1201 acgtctggat atagacgttc ctcaggcctg ggtaatgaaa aattaccaaa actatgttga
     1261 tccatcgtta tgggaaaatg gcattaatgc agccatgttg tcatacaacc tcaacggata
     1321 tcatagtgaa acccctggtc gaaaaaatga cagcatttat gctgctttta acggtgggat
     1381 gaatttgggt gcatggcggc tgcgtgcctc aggcaactac aactggatga ccgattctgg
     1441 cggtaattat gattttaaga atcggtatat acagcgtgat atcgcctcac tacgctctca
     1501 attcattatt ggtgagtctt atacgacggg cgaaaccttt gattccgtca gtatccgagg
     1561 cattcgctta tacagtgaca gccgcatgtt gcctccgaca ttagccagct ttgcgcctat
     1621 cattcatggc gttgccaata ccaacgccaa agtcactatt acgcaaggtg gctataagat
     1681 ttatgaaacg acggtgccgc caggcgcttt cgtcattgat gatctgagtc catcagggta
     1741 cggcagtgat cttattgtta ccatcgaaga atccgatggc tcaaagcgga cattctcgca
     1801 acctttttca tcggtcgttc aaatgttacg ccctggcgtt ggacgttggg atattagcgg
     1861 cggccaggtc ttaaaagatg atattcagga tgagcctaat ttatttcagg ccagctacta
     1921 ctacggtctg aataactatc tgacgggtta taccggtatt cagattaccg ataataacta
     1981 taccgctggt ttgttaggtc ttggtctgaa tacttcggtc ggtgcatttt ctttcgatgt
     2041 gactcactcc aatgttcgta taccggatga taaaacatac caggggcaaa gttatcgtgt
     2101 ttcctggaac aagttattcg aagaaacaag tacttcactg aatatcgcgg cctatcgcta
     2161 ttcgacacag aattatcttg gtcttaatga tgcactaact ctgattgatg aagtgaaaca
     2221 tccagaacaa gatcttgaac cgaaatccat gcgtaattac tcgcgcatga aaaatcaggt
     2281 tacagtcagt attaaccaac cgttgaaatt tgaaaaaaaa gattacggtt ctttttatct
     2341 ttctggtagt tggtccgatt actgggcttc cggacaaaat cgtagcaatt actccattgg
     2401 ctacagtaac agtgcatcct ggggcagcta cagcatcagt gcacagcgtt catggaatga
     2461 ggacggcgac gctgatgata gcgtttatct cagtttcacc attcctattg aaaaattact
     2521 tggcactgaa caacgtactt caggtttcca gagtatcgat actcaaatga gcagtgactt
     2581 taagggtaat aaccaactca acgtcagtag cagtggctat agcgataacg ctcgcgtcag
     2641 ttatagcgtg aatactggct atacgatgaa taaagccagc aaagatttga gttatgttgg
     2701 gggttatgcc agctatgaat caccgtgggg aactctggca ggttcagttt ctgcaaacag
     2761 cgataacagt cgtcaagttt ctctcagcac cgacggtggt tttgtattgc atagtggcgg
     2821 cctgaccttc agtaatgaca gttttagcga ctccgataca ctggcggtag ttcaggctcc
     2881 aggtgctcaa ggagcgcgaa taaattatgg caacagtact atcgatcgat ggggttatgg
     2941 tgtcaccagc gctctttctc cttatcatga aaaccgtatc gcgctggata tcaacgatct
     3001 tgagaacgat gttgaattaa aaagtaccag tgctgtagct gtgcctcgtc agggttctgt
     3061 cgtttttgct gattttgaaa ccgtgcaagg gcaatcagcc attatgaaca tcacacgtag
     3121 tgatggcaaa aatattccat ttgctgcaga tatttatgat gaccaaggca atgtcattgg
     3181 taatgttgga cagggtggac aggcttttgt tcgtggtatt gaacagcaag gaaatatcaa
     3241 cattaaatgg ctcgaagaaa gtaaacctgt aagttgtctt gcgcattatc aacaaagttc
     3301 agaagcagaa aaaatagcgc aatctattat tctgaatgga attcggtgtc agattcagta
     3361 actacaaggg acgtcaaatg ataaaaacaa cgccacataa aatagcgata ctgatgggag
     3421 tattattatc accctcagta tttgcaacgg atattaatgt tgagtttaca gccactgtca
     3481 aagcgacaac ctgtaacatc acacttactg gtaataatgt cacgaatgat ggcaataata
     3541 actacacttt gagaatccct aagatgggcc tggataagat cacgaataaa acgacagaat
     3601 ctcaggctga ttttaaactg gttgccagtg ggtgcagcag tggcattagc tggattgata
     3661 ccactctgac cggaaacgca ttatcaagct catccaagct tattataccg ctgtctggtg
     3721 atttatcttc gacgacaagt aatatcggta tgggtttcaa aaaacggact actgatgatg
     3781 ccactttcct tcaacctaac aacagtgcgg acaagatacg ctggagcaca gaagaaatgc
     3841 agcccgataa aggtcttgaa atgactgtcg cgctacgtga aaccgcagca gggcaaggcg
     3901 tgccggggat ttttcgcgcc ctggcgacgt ttaatttcat ctatcaataa gaaggataat
     3961 taagatgaca tttaaaaatt tacgttatgg attatccagc agtgtcgttt tggctgcctc
     4021 attgttcagc gtaccctctt atgcggcaac tgacagcatt gggctgaccg tgattaccac
     4081 cgtagaaatg ggtacttgta ccgctacatt agtaaatgac tctgatcagg acatttctgt
     4141 tgttgatttt ggtgatgtat atatttctga aatcaatgcc aagaccaaag taaaaacatt
     4201 caaactcaaa ttcaaagact gtgcgggtat ccccaataaa aaagcgcaaa taaaattaac
     4261 caagcgagcc acatgcgagg ggactgctaa tgacggtgca gggttcgcaa atggttccac
     4321 agccacagat aaagcaagtg ccgtcgccgt tgaagtctgg agcaccgaaa ctccggcaac
     4381 agggagagca acacaattta gttgtgtgac accagcatct caagaggtaa ctatttccac
     4441 tgctgccaat gcggttgttt attacccgat gagtgctcgc ctggtcgttg aaaaaaataa
     4501 aaccgtaagt aatgtcactg caggtaaatt ttctgcacct gccacattta cagtaaccta
     4561 taactaacag actgacagat tcaggaacta tatatgcatc ccactcaacg taagctgatg
     4621 aagagaataa ttctgtttct gtcattactg tttcccatta cctgtccagc gatcgctgga
     4681 caggatattg accttgttgc taatgtaaaa aacagcactt gcaaaagcgg aatcagtaac
     4741 cagggtaata ttgatcttgg tgtcgttggg gtgggatatt tttcaggtaa tgttactcct
     4801 gaaagctatc aaccaggtgg aaaagagttc actgtcactg tctcagactg tgcattacag
     4861 ggaactggcg atgtgctaaa tcagttacat attgatttta gagcccttag cggtgtcatg
     4921 gctgcaggct ccaggcaaat atttgccaat gaagtgacaa ccggggctaa aaatgtcggt
     4981 gtcgttctct tttccattca ggacccggcc aatatattca atgttcttag ttcagcggga
     5041 aattctcgtt caatctatcc agtaatgact tctgccctga ataattcgtc atggaaattt
     5101 tatgccagaa tgcaaaaaat cgatcccgcg ctggatgtca tatctggcca ggtgatgagt
     5161 catatattag ttgatgtgca ctacgaataa tatatcaaaa caaacgactg ctattatgac
     5221 agcagcgaat agtgggtaat catgaagaca ttcttcaaat actttgtttt tttagtacta
     5281 tcctgctctt gctttacagc gattagtaag gatacaagtt ttcttgttgg taacaatgcc
     5341 ggggtaggcg atggtaattt caacggcccg agcactgcgg ctcaggtcac ttttagatat
     5401 gcatcaacaa ccaataattt agttttttat aaacctactc aacttggccc gacaggtgtt
     5461 aaattacgct gggaacaatt agatacagcc agcggtggcg gttttcttta ctgtaatgcc
     5521 tctgacaggg caaatccaga tggactaatg gatattgaaa atgccatggc tgattctggt
     5581 aaaacctatg gtggacataa gttatttaaa acctctgtcc caggcttgta ttacactcta
     5641 tttattgcaa aactttggtc tgcatattca acaaaaacaa ctattagcga ctctggttta
     5701 tatattggag ataccacacc acaaaaattc cagtggatca tcacagaccc tgatctacaa
     5761 catatcggct gcgataacgc gatgcggtac gatagattct gggccatcgg cggtgttgta
     5821 cacgatctta cgattgaatt ttatactgat accgatttta accctacaac aaaccaggac
     5881 gtatcattgt ccagtaattc gacatatctt tatgcgttta aggcctacag tcccggttct
     5941 cgtgttgttg atcacagcca tcacctttat atcgactttg atctactcaa tgttaaatta
     6001 actaacccca cctgctttac tgctgtactt accggaaaat cagtaagtgg ttcgacggta
     6061 aaaatgggag aatatgcacc agggcagatt aaaaatggtg ctactccggt tccttttgat
     6121 atttctctcc agaactgtgt tcgcgttggc gatatagaga caaagctatc ttcagggaag
     6181 ctgggtacag aaaataagca actcctgggt aacactctta ctggaagcgg cgcagcaaaa
     6241 ggcgttgcgg tattgattga aggattagca aacagaaaaa gtgccctaat gatactgaaa
     6301 ccgaatgatt caacttctgt ttataaagat aatacgggtc aggctcagga tcacgattca
     6361 gatgcaattt acccagaaga tcaaggtata acatatccac ttcatttcca ggcgacgtta
     6421 aagcaagacg gaaatattgc tatcgaacca ggagaattta aagccaccag tactttccag
     6481 gtaacctatc cctgataaca ttgtggcacc gtctggtaat ggcggtgcca caatagataa
     6541 atcattgccc ccaccaatgt ggctgataaa atgccagaca gcacgacatt acttatcagg
     6601 atacgttatg aaaccaacca ctattgcttc attgcaaaaa tgcaaacaag aaaaaaaacg
     6661 tttcgcgacc atcactgctt acgattatag cttcgcaaaa ctgtttgccg aagaaggact
     6721 taacgtcatg ctggtcggcg attctctggg catgacggtt caggggcatg actccacgct
     6781 accggttacc gttgctgata tcgcctacca cactgccgcc gtgcgtcgcg gcgcgccaaa
     6841 ctgccttctg ttagcagacc tgccatttat ggcgtatgcc acgccggaac aagcctttga
     6901 aaacgccgca acggtaatgc gtgccggagc caacatggtc aaaattgaag gcggtgagtg
     6961 gctggtagaa accgtacaaa tgctgaccga acgtgccgtt ccagtttgtg gtcatttagg
     7021 cctaacccca cagtcagtaa atattttcgg tggctacaaa gtccaggggc gcggcgatga
     7081 agcggccgat cgactgctca gcgatgcatt agccttagaa gctgctgggg cacaactgct
     7141 ggtactggag tgcgtgccgg ttgaactggc gaaacgcatt accgaagcac tggcgatccc
     7201 ggttatcggt attggcgcag gcaacgtcac tgacgggcaa atcctcgtga tgcacgacgc
     7261 gttcggcatt accggcggtc atattcctaa attcgccaaa aatttcctcg ccgaaacggg
     7321 cgacatccgc gcggctgtgc ggcagtatat ggctgaagtg gagtccggcg tttatccggg
     7381 cgaagaacac agtttccatt gaggagtgac gttgtgttaa ttatcgaaac cctgccgctg
     7441 ctgcgtcagc aaatccgccg cctgcgtatg gaaggtaagc gcgtggcgct ggtgcccacc
     7501 atgggtaacc tgcacgacgg ccatatgaag ctggtcgacg aagccaaagc ccgcgccgat
     7561 gtggtcgtcg tcagtatttt cgttaaccca atgcagtttg accgcccgga agatctggca
     7621 cgttatccac ggaccttgca agaggactgc gaaaagctga acaaacgtaa agtggattta
     7681 gttttcgcgc cttcggtaaa agagatctac ccgaacggta cagaaaccca cacctatgtt
     7741 gatgttcctg gtctttccac catgctggaa ggtgccagcc gtccgggaca ttttcgcggc
     7801 gtttcgacta ttgtcagcaa gctgttcaac ctggtccagc cggacatcgc ttgcttcggc
     7861 gaaaaggact ttcagcaact ggcgctgatc cgcaaaatgg ttgccgatat gggctttgat
     7921 attgagatta tcggcgtacc gattatgcgc gccaaagacg gtctggcgct gagttcccgt
     7981 aacggttatc tgacggcgga acaacgcaaa attgcgcccg gtctgtacaa ggttttaagt
     8041 tcgattgccg acaaattgca ggctggcgaa cgggatctcg atgaaattat tgccattgcc
     8101 gggcaagaac tgaatgaaaa aggcttccgc gccgatgata ttcagattcg tgatgccgac
     8161 acattgctgg aagtctctga aaacagcaaa cgcgcagtga ttctggtagc cgcctggctt
     8221 ggcgatgctc gcctgatcga caacaaaatg gtcgaactgg cgtaatgctt aactggcgct
     8281 acggctgatg gcgccagcta ttcattgcca cgttatctgt aagcgattat tcataacgca
     8341 gacaacttag ttaatcattt tatcaatctc agcaagaggt aaatttgcca tctctgcaac
     8401 gtcttcccga gacattcctt tactcagaag acgcagggcc agtcttacct tctggagtcc
     8461 ctctttatat cccttctgaa atcccttctc ttcaaaccac tgcgccaacg tctgcctggt
     8521 ttctctccgt gtctttattt ccatatgttc ccttaattat ctggtcagtt aacaataaca
     8581 attatctggc tataactgat aatgcagata acttagctaa tcagcttatc aacctcagca
     8641 agaggtaaat ttgccatctc tgcaacgtct tcccgagaca tccctttact cagaaaacgc
     8701 agggcgaatt cctgtcttac ttcctgtcta acctcttgta ttacctcctg tcttacctcc
     8761 tgtcttacct cctgccttcc cctctcttca aaccactgcg ccagcgtcat catagactcc
     8821 cctcccgttt ccctgtctcg caacacacca taaaacaaat ccgcttgttc agtatgaccg
     8881 cgttgcagca tatagttttg catggcaact aactgacttc cgctagtgta cccttcgtca
     8941 atcagcgtga ccagttgctc cagcaataac attaagtcgc gctggcgaat atgcttttgc
     9001 agtagttcga gaatcgcaat ccgccggtgc tgcatgattt cgtcatccgg cgtgatggtg
     9061 atatccacca gcgggaaagg gttgttgtaa acgcgtcgcg ccagctccgg cgagtaaaac
     9121 atatcaaacc agcacattga gagcggataa ggtgtggcct cgccctgata aaacagaatc
     9181 ggcaccacca gcggcagctt atcgtgatcc gcctccagat gccggtgcat ggcggcaatg
     9241 gcatagcgca tcatgcgaaa ggtcattttc ttatccggct tgctttggtg ttcaatcaca
     9301 acatgcagat aacccgtact accctgcatc tgtacggaat agagcacgtc agtgctgtgc
     9361 cctttcaggc tttcttcaat aaaactcccc gactcaagat gcagcgtgtc gaggtcacac
     9421 agttcgcgta gctccgctgg caaatggata tccagaaagt cgcgagccgt ttccgcatgc
     9481 attaaaaatt gtttaaacac cgcatcatgc ggtgtggtac tcggtgcatc catctcccct
     9541 gccttccgtg ttgtcttgcg tgaaggcagt atccagtcaa ggtgtgccgt cgtcagccgc
     9601 ctctttagtg tttaccgagc agcgttcaga taaatttcaa ttggttacag gcgttacatg
     9661 gcacgcttcg gcacggtacg caaactgctg atgcaaatgc cgtcagatga gtagacacta
     9721 aacaaaaatc gggcaatact gcgtgagaat ttcctcaaag caggcatcgc ctgcttcgtt
     9781 aacgacaggg tagaaaggta gaagttatga ttcgcacgat gctgcagggc aaactccacc
     9841 gcgtgaaagt gactcatgcg gacctgcact atgaaggttc ttgcgccatt gatcaggatt
     9901 ttcttgacgc agccggtatt ctcgaaaacg aagccattga tatctggaat gttaccaacg
     9961 gcaagcgttt ctccacctat gccatcgcgg cagaacgcgg ttcgagaatt atttctgtca
    10021 acggtgcggc ggcccactgc gccagcgtcg gcgatattgt catcatcgcc agcttcgtta
    10081 ccatgccaga tgaagaagct cgcacctggc gacccaacgt cgcctatttt gaaggcgaca
    10141 acgaaatgaa acgtaccgcg aaagcgattc cggtacaggt tgcctgattg ttatccctgc
    10201 ggctggttac tcaccagccg cgacatcgtc tccagcgaat ctgttcttaa gatataaagt
    10261 cgttttagta acaacggatt atcccccggt tttactttgc ctttcatggt cgtcacagcc
    10321 aggtgaaatc cggcttcttt tgctgccttc acggctttgt cattaaatcc gccaaatgga
    10381 taagaaagat acaaaacatg tggattaaat tgcgccagag cgcggcgtga acgtgcaaaa
    10441 tcaaacagaa tattgtgctc actacggctc agcaatatag gccgccgata accatctatc
    10501 cgatgcaaaa aatgagtatg tgactggaaa tcaaatacat cgcgaatttc gttaagttca
    10561 gaaacgctca taaattgcag cgattttggg ttccatttct gcgggtgacg tttgatgcgc
    10621 gaggtaacaa taaacgccgt cgccttcatg ccatattgtt tcaacacagg atacgcatag
    10681 cggctcaccg acttgaggcc atcatcaaag gtaatcacca ccgctcgcgc agggagattg
    10741 atcttattct tcacgtaacc ttccagctgc gccatgctca gtgtcgcgta tcccctgtcg
    10801 cgcagccagg ccatctggtt attgaaagcg cgtaccgagg tggtcgtcga agtatggcga
    10861 aaacgggtgt tttcttcatc gcgcaaaata tggtgatagg ttagcaccgg caggccatta
    10921 tcgggttggg catccagcgc gctgatatag gccagtcgat cgccaatacg gatctgatac
    10981 caggtttgat ttaacctgtc tttcagttta tgcaaaatcg ggtagcgcaa attgtccgcc
    11041 agtactccaa atggcgcact gcccacactc ggcgcgttat agacctgcgt atctttccag
    11101 gtaactaagt tctgattact cagcggcttg ttgaggtcac ccaaaccgtc ttcaactttt
    11161 tgtcgcccct gaaccggctc gagatgccct ttatcgataa accctttgcc aaggccaaaa
    11221 ttaaatacgt aataacttgc ggcagtgggc tccacggcaa tgatttgtcc ggcccgaata
    11281 ttccccaccg tcaccatctt gtcaccaatt tgcgcccaga ccgcggcatt ttcgatggtt
    11341 tgcatataac gggcaggtaa cgcggcactg acacttgcgg taaacagcat cagcaggaga
    11401 ataacagctt gtttgtacat gaaaaaataa ccgagggtga aacaacgctt ttattgtagc
    11461 aaaagcgtca tcagaatcgc gggttaatac tcatacaaat tatgtttgag gacgaaaggc
    11521 ggattttttt gttgctggtg ccaaagacta ctcacctccg gcgcacccag ttcgacaata
    11581 cgctcgcgaa acgctgaact ggtcatggtt tcacggcaag ccatcatctg ttcaattaac
    11641 ggtaacgtga caccagaaat cacttcgcaa cgggagtgtt tgtggcttaa taatgaagcc
    11701 acgcggtacg gtggcgctcc ggctatatcg gttaagaaaa taacgccttc accagagtcc
    11761 gtttcatgca aggcatcgca catcatgcgg ctgagcatat tagagcttaa tccgcgccag
    11821 aaattcacgg cccggcactg aagaagtgcc ccatgttttt tctccagcgc atccagtatc
    11881 tcttgcgccc ggtcatcgtg acaggtaatt acccaaccta acatcgcctt cctccttagc
    11941 cgaaaatcag tctaccctga ccattgttga taaagggtga tagccgtcaa aactcctctc
    12001 ccccaaatcc agaggagagg aaatagcctt agctacgcaa accacgtcca cgttggatca
    12061 gcgaccaaca gatcaaataa aacgccacaa taaagaccac cagtacgcca aaggtagtga
    12121 ccagcggaac atcattgata ccgaggaagc cgtagcggaa accactgatc atataaacga
    12181 ttgggttcag gtgcgacagc ccttgccaga acggcggcaa caaagtcagg gagtaaaaca
    12241 ccccgcccaa atacgtgagt ggcgttaaca caaaggttgg caccaggctg atgtcatcga
    12301 acgttttagc aaacacgccg ttcagcaaac ccgcaaggga gaatagcacc gcagtgagca
    12361 ccagggttaa ggcaacgaat acccacgaat gcacctgaaa cggcacaaaa aacagtgaaa
    12421 ttgccgtcac cagaatacca acaaacagac cacgcgccac gccaccaccg acatatccgg
    12481 caataatgac atgagtcgga accggcgcta ccagcagctc ttcaatatta cgctggaact
    12541 tggcaccaaa aaatgatgac gcgacgttag cgtaggcgtt ggtgatcacc gacatcatga
    12601 tcagccccgg tacgatgaac tgcatgtagc tgaagccatg catatcgcca atacgcgaac
    12661 caatcaggtt accgaagata ataaagtaaa gggtcatggt gatgactggc ggcaccagcg
    12721 tctgcaccca gatacgcata aagcgatgga tctctttcgc ccagatgctt tttagcgcca
    12781 cccagtaaag atgcatcatg tgcgatctcc ttgtttttca ttaaccagtg aaacaaacag
    12841 ctcttccaga cggttagctt tgttacgcat acttaatacc tgaatgccct gctcacttaa
    12901 ctgcgtaaat acgctgttga tgccctgctc acgcagcact tcaacttcca gcgtggcggt
    12961 atctaccagt cgatactgat agccatcgag cttcggtaac ggacttttcg gtgcgagatc
    13021 gagaataaag gtttctgatt tcagcttcgc cagcagcgcc ttcatcgaag tattttccac
    13081 caactcaccg tgttgaataa tgccgatatt gcggcacagc atttctgctt cttccaggta
    13141 gtgtgtggtg agaatgatgg tggtgccttt gtcgtttaaa tccttcaaaa agccccacat
    13201 tgagcggcga agttcaatat ccacgcctgc ggtcggttcg tcgagaatca gtagtttagg
    13261 ttcatgcatt aacgcacggg caatcattaa acggcgcttc atcccgccag ataacatacg
    13321 cgcacgttcg ttgcgttttc cccatagatc gagttgttta agatactttt cgctgcgaat
    13381 gtacgcttct ttgcgctcca caccgtagta ccctgcctga ttcaccacaa tttgctgcac
    13441 ggtttcaaac gggttgaagt taaattcctg tggcaccaga cccaactgac gtttagcgtt
    13501 tacgacatct ttctcgagat cgtaaccaaa tacgctgacc cgcccggagg ttttatttac
    13561 cagagagctg ataataccga tagtggtcga ttttccggcc ccgttcggcc cgagaagcgc
    13621 ataaaaatca cccgcttcga cctgcaaatc gattccacga agcgcctgaa cgccgcctgg
    13681 ataggttttt ttaagctgtt gaagttccag tgcaatggtc ataaattttt acttacctta
    13741 cgttcttaca ctttatctgt ggtttaaatc gtcccggagt tgccctatat tagccaaacg
    13801 taattatttg gttacaggtc gttaacctcc atgaaagaca tagatacact catcagcaac
    13861 aatgcactat ggtcaaaaat gctggtggaa gaggatcccg ggttttttga gaaactggca
    13921 caagcgcaaa aaccgcgctt tctatggatt ggatgttccg acagtcgcgt tcctgcagaa
    13981 cgtttaaccg gtcttgagcc gggcgaactc tttgttcacc gtaatgttgc taacctggtc
    14041 attcacaccg acctgaactg cctttccgtg gttcagtatg cagtggatgt actcgaagtt
    14101 gaacacatta ttatctgtgg ccactacggt tgcggcggcg tacaagccgc agttgaaaac
    14161 ccggaactgg ggcttatcaa caactggctg ctgcatatcc gcgatatctg gttcaaacat
    14221 agctcattgc tcggcgaaat gccgcaagag cgccgtctgg ataccttgtg tgaactgaac
    14281 gtcatggaac aggtgtataa cctgggccac tccaccatta tgcaatcagc gtggaaacgc
    14341 gggcagaaag ttaccattca cggctgggcc tacggcattc acgacggctt gctgcgtgat
    14401 ctggatgtca cagcgactaa ccgcgaaacc cttgagcaac gttaccgtca cgggatctcc
    14461 aacctcaagc tgaaacacgc caaccacaaa taaaaatgcc atgccggatg caacacatcc
    14521 ggcaacttca cacttactcg tccagcagaa tcactttgcc gatatacggc agatgacggt
    14581 aacgctgtgc gtaatcaatg ccgtaaccca ccacaaactc atccgggatc gagaaaccga
    14641 taaattctac cgggacgttc acttcacgac gggacggttt atccagcagc gtacaaatcg
    14701 ccaacgactt cggttcgcgc aggcttaaga tctcacgcac tttcgacagt gtattccccg
    14761 agtcgatgat atcttcaaca atcagcacgt ccttgccacg gatatcttca tccagatctt
    14821 tgaggatttt cacatcacgg gtggtggaca tgccgctacc gtagctggag gcggtcataa
    14881 agtcgacttc atgagatacc tgaacttcac ggcacaggtc cgccataaac ataaatgagc
    14941 cacgcagcag acccaccagc accatatcgc tgccgctgtc tttgtaacgc tcagtaatct
    15001 gacgacccag ttcggcgata cgcgctttaa tctccgcttc ggggatcatt acttctacag
    15061 tatgtttcat atctataacc atatgattta aaaacaaatc acttcgcacg ggcaggtttt
    15121 ggcaccgcaa caaagcgcaa gacacgcagt ataccagtaa aactattcat tcgcggaatc
    15181 tgttaataaa gctcgaattg tgatgacgat cacacatgtt aaaacctata attaattgct
    15241 actaaaatat taatgaattg aaatggtgtc tctttatggc aattaacaat accggctcgc
    15301 ggcgattact cgtcacgcta acagcccttt ttgcagcgct ttgcgggctg tatctactca
    15361 ttggcggagg ctggctggtc gcgattggcg gctcctggta ctaccctatc gctggccttg
    15421 tgatgctcag cgtcgcctgg atgctgtggc gcagtaaacg cgccgcgctt tggctgtacg
    15481 cagccctgct gctcggcact atgatttggg gcgtctggga agttggtttc gacttctggg
    15541 cgctgactcc gcgcagcgac attctggtct tcttcggcat ctggctgatc ctgccgtttg
    15601 tctggcgtcg cctggtcatt cctgccagcg gcgcagttgc cgcactggtg gtcgcactgc
    15661 tgattagcgg tggtatcctg acctgggccg gatttaacga tccgcaggaa atcagcggca
    15721 ccttaagcgc cgataccacg ccagcagaag ccatctcccc cgttgccgat caggactggc
    15781 ctgcctatgg tcgcaatcag gaaggtcaac gcttttcacc tctgaaacaa attaacgctg
    15841 ataacgtcca caagctgaaa gaagcctggg tgttccgtac tggcgatgtg aagcagccga
    15901 acgatccggg cgaaatcacc aatgaagtga cgccaattaa agtgggcgac accctttacc
    15961 tgtgtaccgc gcaccaacgt ctgtttgcgc tggatgccgc cagcggtaaa gagaaatggc
    16021 attacgatcc tgagctgaaa accaacgagt ccttccagca tgtaacctgc cgtggcgtct
    16081 cttatcatga agccaaagca gaaactgctt cgccggaagt gatggcggat tgcccgcgtc
    16141 gtatcattct tccggtcaat gatggtcgtc tgattgcaat taacgctgaa aacggcaagc
    16201 tgtgcgaaac cttcgccaat aaaggcgtgc tcaatctgca aagcaatatg ccggacacca
    16261 aaccgggtct gtatgagccg acttcgccgc cgattatcac cgataaaacc atcgtgatgg
    16321 ccggttcagt caccgataac ttctcaaccc gtgaaacatc tggcgtgatc cgtggttttg
    16381 atgtcaacac cggggagctg ctgtgggctt ttgatccggg cgcgaaagat ccgaatgcga
    16441 tcccgtctga cgaacatacc tttaccttta actcaccaaa ctcgtgggca ccagcggcct
    16501 atgacgcgaa gctggatctg gtctatctgc cgatgggcgt caccacgcca gatatttggg
    16561 gtggtaaccg cacaccggaa caggaacgtt atgccagctc gattctggcg ctgaatgcca
    16621 ctaccgggaa actggcgtgg agctaccaga ccgttcacca cgacctgtgg gatatggatc
    16681 ttccggcaca gccgacgctg gcggacatca ccgttaatgg tcagaaagtg ccgattattt
    16741 acgctccggc gaaaaccggc aacatttttg tgctcgatcg tcgtaatggt gaactggtgg
    16801 ttccggcccc ggaaaaaccg gttccccaag gtgctgccaa aggcgattac gtgaccccta
    16861 ctcaaccctt ctcggaactg agcttccgtc cgacgaaaga tttgagcggt gcggatatgt
    16921 ggggtgccac catgtttgac cagttggtgt gccgcgtgat gttccaccag atgcgctatg
    16981 aaggcatttt tacgccgcca tctgaacagg gtacgctggt cttcccgggt aacctgggga
    17041 tgttcgaatg gggcgggatt tccgttgatc cgaatcgtga agtggcgatt gccaacccaa
    17101 tggcactgcc gtttgtttcg aaactgatcc cacgcggtcc aggcaacccg atggaacagc
    17161 cgaaagatgc caaaggcacg ggtacggaat ccggcatcca gccacagtac ggtgtaccgt
    17221 atggtgtcac gctcaacccg ttcctctcac cgtttggtct gccatgtaaa cagccagcat
    17281 ggggctatat ctcagcattg gatctgaaaa ccaatgaagt ggtgtggaaa aaacgtattg
    17341 gtacgccgca ggacagtatg ccattcccga tgcctgtacc ggtgccgttc aatatgggta
    17401 tgccgatgct tggcgggcca atctccacgg cgggtaacgt gctgtttatc gccgctacgg
    17461 cagataacta cctgcgcgct tacaacatga gcaacggtga aaaactgtgg cagggtcgtt
    17521 taccagcggg tggtcaggct acgccaatga cctatgaagt gaatggcaag cagtatgtgg
    17581 tgatctccgc aggcggtcac ggttcatttg gtacgaagat gggcgactat attgtggctt
    17641 atgcgctgcc ggatgatgtg aagtaaccat aatgcccgga gagatccggg catatttccg
    17701 aatacggtct ttttataccg taaaccctaa catcatcccc gtatcttcat gctccagcag
    17761 atggcagtgc gccatataag catgttcttt cggtgcatcg tgattaaact tcaccagcac
    17821 ttcgctgaca ttaccttcta ccttaacggt atctttccag cctgcgcgat gcgtcgctgg
    17881 cggtttgcca ttttctgaca agatacggaa ctgcgtaccg tggatatgga acggatgcag
    17941 catcatgtcg cccacgccag agataaccca acgttcgtac tgccctttcg ccgccgcaaa
    18001 catcggctta ttcatatcaa acgcctgacc gttgattttg ttggcatggt ggaaatcgaa
    18061 cttcccgctg tggttcatat gattcatatt gccgtgcccc atatggccca tcatctggct
    18121 gtgatccatc ccggccatgg cctgatcgcc atatttctcc atcagcatct gcatccccat
    18181 catatcgagc attgggtcca tagaaagttg cagattgcgt accgtcagcc cttccagcga
    18241 aggtaacgca ggcaggctac ttaatgtgtc tggcaaagca ccagaggcac taatggcaat
    18301 cggctgaatc cgcattaccg gatgcggctt gtcaaacggc gcaatcgcca tccccatctg
    18361 gctgaccggc agcgtcacca ggtcaaaggg tttgttatcg ttaacctcca ccagcacttc
    18421 aaaacgctcg cccatcagca ctggcagctc gttcaccttc accggttcag gtagcagacc
    18481 gccgtcgctg gcaatcacat aaagcgggcg attgtcgctg gtggcgaaat tgagcgagcg
    18541 ggcattacag ccattgagca aacgcaggcg cagccagcca cgcggggcag cgtgttgcgg
    18601 gtagattgca ccgttggtca gcaacgtatc gccaaaccaa cccacagcgg cggtcatcac
    18661 atccagttga taatcaatct gcccgtctgc gttaaatttt ttatcctgaa caatcaccgg
    18721 gacatcatca ataccccact gttttggcag cattaatttc aggatctcgt catcttcaat
    18781 caccaccaaa ccagccagcc ccatcgccac ctgacgtccg gtcttgccat gttgatgtgg
    18841 atggaaccag caggtagcgg caggttgatc aacgttcaac gtcaccgagc gcttgccacc
    18901 tggcggaata attccctgcg ggccgccgtc cacttcaccc ggtacttcca gcccgtgcca
    18961 gtgcaacgtc gtctcttccg tcagttggtt gtagatatca accgttaccg ctttgccgcg
    19021 ctgtaatttc accgccggcc ccagcagatt gccgttatag ccccaggtag ttgcggtttt
    19081 cccgccaaag gtggactgac ctgcgccaat agttaactga atgcgattac gggcatcggt
    19141 cgtgagcaaa tcagggattg gtaacgttgg gcgttccgcc gcaaatactg cgcggctcca
    19201 cagcggcaag gctgaagcca cccccagcgc gacggaatat ttcaagaaat cacgacgttg
    19261 catagttatt tccttattct taagcaggcg aaacaaaatc aaacgttgag catagtcctt
    19321 ccccttacgg gaaggtcaag ccgcagacac aatttaatcg acaataatgg tcgtcgcctc
    19381 gctcgcagtg tgctaacgtt tatcttcttt aagcccctgg tagaagcaat gaagacgttt
    19441 ttcagaacag tgttattcgg cagcctgatg gccgtttgcg caaacagtta cgcgctcagc
    19501 gagtctgaag ccgaagatat ggccgattta acggcagttt ttgtttttct gaagaacgat
    19561 tgtggttacc agaacttacc taacgggcaa attcgtcgcg cgctggtctt tttcgcccag
    19621 caaaaccagt gggatctcag taattacgac accttcgaca tgaaagccct cggtgaagac
    19681 agctaccgcg atctcagtgg cattggcatt cccgtcgcta aaaagtgcaa agccctggcc
    19741 cgcgattcct taagcctgct tgcctacgtc aaataatccc tgataccttt ttgtagaaat
    19801 attgaccgtg catccgcggt caatgttagc tattatgttg cgccattttt ttacgggtgt
    19861 taacaaagga ggtatcaacc catggccgaa aaaaaacagt ggcatgaaac gctacacgac
    19921 cagtttgggc agtactttgc ggtagataac gttctgtatc atgaaaagac cgatcaccag
    19981 gatctgatca tttttgagaa cgccgcattt ggtcgcgtaa tggcgctgga tggcgtagta
    20041 caaaccaccg agcgcgacga gtttatctat catgagatga tgacccatgt tccgctactg
    20101 gcccacggtc acgcgaaaca tgtgctgatt atcggcggcg gcgacggtgc catgctgcgt
    20161 gaagtaaccc gacataaaaa cgttgagtca atcacgatgg tggaaatcga tgcgggtgtc
    20221 gtgtcgttct gccgtcagta tcttcccaac cataacgccg gtagttacga cgatccgcgc
    20281 tttaagctgg tgatcgacga tggcgtcaat ttcgttaatc aaaccagcca gacctttgat
    20341 gtcattatct ccgactgcac cgatcctatc ggtcccggcg aaagtctgtt cacttcggca
    20401 ttttatgaag gctgcaaacg ttgcctgaat cctggcggta ttttcgttgc gcagaacggc
    20461 gtctgctttt tacagcagga agaggccatc gacagtcatc gcaaactcag ccattacttc
    20521 agcgatgttg gcttttatca ggcggcgatc ccgacctatt acggcggcat catgaccttt
    20581 gcatgggcga cagataacga cgccttacgc catctttcaa ccgaaattat tcaggcgcgt
    20641 tttctcgctt ctggcctgaa atgccgttat tacaatccgg cagtccatac ggcagctttt
    20701 gccttacccc agtatctgca agatgcactg gcttcacagc cgtcctaagg agaagataag
    20761 aaattgaaaa aactgaaact gcatggcttt aataatctga ccaaaagtct gagtttttgt
    20821 atttacgata tctgctacgc caaaactacc gaagagcgcg acggttatat tgcttatatc
    20881 gatgaactct ataatgccaa ccgtctgacc gaaatcctgt cagaaacctg ttccattatc
    20941 ggggccaata ttcttaacat cgcccgccag gattacgaac cacaaggtgc cagcgtcact
    21001 attctggtga gtgaagaacc ggtcgacccg aaacttatcg acaaaacaga acaccccggc
    21061 ccactgccag aaacggtcgt tgcccatctc gataaaagtc atatttgcgt acatacctac
    21121 ccggaaagtc atcctgaagg cggtttatgt accttccgcg ccgatattga agtctctacc
    21181 tgcggcgtga tttctccgct gaaggcgctg aattacctga tccaccagct tgagtccgat
    21241 attgtgacta ttgattatcg cgtgcgcggt tttactcgcg acattaacgg tatgaagcac
    21301 tttatcgacc atgagattaa ttcgattcag aactttatgt ctgacgatat gaaggcgctg
    21361 tatgacatgg tggatgtgaa cgtctatcag gagaatatct tccataccaa gatgttgctt
    21421 aaagagttcg accttaagca ctacatgttc cacaccaaac cggaagattt aaccgacagc
    21481 gagcgccagg aaattaccgc tgcgctgtgg aaagaaatgc gcgagattta ttacgggcgc
    21541 aatatgcccg ctgtttaacg tctcaggcgg agcacggagg ctccgcctga tttacttact
    21601 tctgctgcac gaaattacgg taagccgcca ccacctgaag aaaatcctca acgccacaca
    21661 gtgacaagct ctcttcatcg tagtagttca tcccctcttc catttcatcg ccagcgaatt
    21721 ccagttgatt ggcgcgaacc atcacctctt caccatccat ccatagcgtg tattcatgcc
    21781 ctgcccgttg ccaggaacgt tcgctacctt tcaatgcgtg cgccgcttgt tccacttcat
    21841 caagcaaggc caggttttct ttcacctctt cattaaacca gtgcccgacc acttcatgcc
    21901 ccatggacat gcgcaccttt accactccgg taatatcgcg cagaaattcg taatccatag
    21961 tgtgttcctc tgctcccggc aatgcgccga aatcgcattg cgccattgat gtaattatcg
    22021 cagtcttacc ggagaaaaac agcggaacgg cgatggttta gaaataaaaa atgcccggga
    22081 atctcattca ccgggcattg tgtcgtttat gcgcagcgcg tgcgcttact tattaaaccg
    22141 cagtctggaa aatcaccccg tccgcttttt cggtgtactg agaaagctgg tcgaagttca
    22201 gataacggta agtatcaacg gcagttttat ctacttgcgc cacgtaggtc tggtactctt
    22261 ccggcgtcgg cagtttgcca atcagcgccg caacagcagc cagttccgca gaagccagga
    22321 agacattcgc gccagtaccc aaacggttcg ggaagttacg ggtagaggtg gaaaccaccg
    22381 tcgcaccgtc tgctacacgc gcctggttac ccatacacag ggaacagcca gggatctcga
    22441 tacgagcacc actcttaccg aagacgctgt agtagccttc ttcggtcagc tgcgcagcgt
    22501 ccatacgcgt tggcggtgcc acccacaggc gggtcggcaa ctgacctttg tgcgcatcca
    22561 gcagtttacc agcagcacgg aagtgaccga tgttagtcat gcaagaaccg ataaatactt
    22621 cgtcgatctt ctcgccctgt accgcagaca gcggacgtgc gtcgtccggg tcattcggtg
    22681 cacagaggat tggctctttg atatccgcca gatcgatgtc gatcactgcc gcgtattccg
    22741 catctgcatc ggcttccagc agctcaggat tcgccagcca tttttccatg ccctgaatac
    22801 gacgctccag ggtacgacga tcgccgtaac cttccgcgat catccacttc agcaggacga
    22861 tgttagagtt caggtattca atgatcggtt ctttgttcag cttgatggta caaccggcag
    22921 cagaacgctc ggcagacgca tcggtcagct caaatgcctg ctcaactttc agatccggca
    22981 gaccttcaat ttccagaata cggccagaga agatgttttt cttacctttc ttctcaacgg
    23041 tcagcagacc ttgtttgatt gcatacagcg gaatggcgtg taccagatcg cgcagggtga
    23101 tacccggctg cattttgcct ttgaagcgta ccagaacgga ttccggcata tccagcggca
    23161 ttacgcctgt tgcggctgca aatgccacca gaccagaacc cgccgggaaa gagataccga
    23221 tcgggaaacg ggtatgggag tcaccaccgg taccgacggt atccggcagc agcatacggt
    23281 tcagccagga gtgaatgacg ccgtcccccg gacgcagcga cacaccgcca cggttcataa
    23341 tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg cttcggatac gccgcggtgt
    23401 ggcagaaaga ctgcatcacc aggtcagccg agaagcccag gcacgccagg tctttcagtt
    23461 catcacgggt catcgggccg gtggtgtcct gggaacctac agaagtcatt ttcggttcac
    23521 agtacgcacc cggacgaatg cctttcacgc cacaggcacg gcctaccatt ttctgcgcca
    23581 gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg acggaacaca tcactatgcg
    23641 gcaggccaag tgcttcacgc gctttggtgg tcaggccacg cccgataatc agcggaatac
    23701 ggccgccagc acgcacttca tcaatcagca cgtcggtttt cagttcgaag gttgccagca
    23761 gttcgccggt ttcgtggtta cgcacttcac ctttgtacgg gtaaacgtca attacgtcgc
    23821 ccatgttcag gttagagacg tcgacttcga ttggcagtgc acccgcgtct tccatcgtgt
    23881 taaagaagat tggtgcaatt ttaccgccga ggcacaaacc accgccgcgt ttgttcggca
    23941 catgtggaat atcatcgccc ataaaccaca gaacggagtt cgtggcggat ttacgcgatg
    24001 accccgtacc cacaacgtca ccgacgtacg ccagcgggaa acctttctgt tgcagagctt
    24061 caatctgttt gatcgggcca acaacaccag gctgatctgg ctcaatacct tcacgggcgt
    24121 ttttcagcat cgccagcgcg tgcagtggga tatccgggcg tgaccacgca tccggtgccg
    24181 gagaaaggtc atcggtgttg gtttcgccgg tcactttgaa gacggtaacg gtcagttttt
    24241 cagccagcgc cgggcgattc aggaaccatt cggcatccgc ccaggactgc attacctgct
    24301 tcgcatattc gttacctgct ttggctttct cttctacgtc atagaagtta tcgaacatca
    24361 gcagtgtgtg agaaagtgct ttggcagcga tcggtgccag tttggcatca tccagcgcgt
    24421 cgatcagcgg atgaatgttg taaccaccct gcatagtgcc cagcagttca atggcttttt
    24481 ccggagtcag cagtggggat ttggcttcac ctttggcgac agcagccagg aagcctgctt
    24541 tgacataggc ggcttcatcg acgccagggg gaacacggtt ggttaacaga tctaacagga
    24601 attcttcttc gcccgcgggc gggtttttca gcagctctac aagcgcggcc atttggtttg
    24661 catccagggg tttgggcgca atcccctcag cggcacgctc agctacgtgc ttacggtatt
    24721 cttctagcac gacggttctc ctcgctctca ttgtcatagt gcggcaggcg attctcttca
    24781 cgctcctgtg agacagcagt ttgtagggta aatgcccagt accgcaacgg gcagaatagc
    24841 aggattttga cgatgtgtta atctgtttac aaaaaagcaa cataaaaaag tggctgaatc
    24901 gttaaggatg gtctagagat tatttccctc cgtaaaaact tcgttaattc gcatggtgat
    24961 aatcaccttt ctcaacgaat cccaagatta gtcaaaattt aaactacggc ctctttatac
    25021 tcggattcac agcacctgcg ggtggcagtt cgccgaccat tgcgatttcc ttgagatccg
    25081 aattatgaaa atgactttgc cgtttaaacc ccatgtgctg gcgctaattt gcagtgccgg
    25141 gctctgtgcc gcctctgccg ggctatatat aaaaagccgc acagtggaag cgcctgtgga
    25201 agcgcaatcg acacaacaga ctgcgcctga catctccgca gttacgcttc cagcaacggt
    25261 ttccgtgcct cctgtaacac ctgccgtcgt caaatccgca ttcagcactg ctcaaataga
    25321 tcaatgggtt gcacctgtcg cgctataccc cgactctctg ctttcacaag tgttaatggc
    25381 atcaacctat ccggcaaacg ttgctcaagc agtgcaatgg tcgcacgata atccacttaa
    25441 acaaggcgat gctgctattc aggcggtatc tgaccagccc tgggacgcca gcgtcaaatc
    25501 actggtggcc tttccacagt tgatggcatt gatgggcgaa aacccgcaat gggtgcaaaa
    25561 cctgggcgat gcttttctgg cgcagccgca ggacgtgatg gactcggtac agcgattgcg
    25621 gcaactggcg caacaaaccg gctcgctgaa gtcatcaact gaacaaaaga tcatcacgac
    25681 aacgaaaaaa gtcgtaccgg taaatcagcc cgccaacgcc cctgccacac aatcaaatac
    25741 cgtttctacg tccagccccg tcgtcgcaga acctgcaccg accgtaataa ccattgagcc
    25801 agccaatccg gatgtggttt atattccgaa ctacaaccca aacgtggttt acggcagttg
    25861 ggccaatacc gcttatccac cagtttatct gccgccgcca gccggagaac cgtttgttga
    25921 cagctttgtg cgcggattcg gctatagcat gggcgttgct accacatacg cactattcag
    25981 cagcatcgac tgggacgacg acgatcatga ccatgaccat catcatcatg acgatgatga
    26041 ttatcatcac cacgatggcg gtcatcgtga cggtaatgac tggcaacaca acggcgacaa
    26101 catcaatatc gacgtcaaca atttcaaccg tatcaccggt gagcatctta ctgataagaa
    26161 tatggcatgg cggcacaatc caaactaccg taatggtgtg acctatcatg atcaggatat
    26221 ggcaaagcgg tttcatcaaa ccgatgtcaa cggcggaatg agcgccacgc agttacctgc
    26281 tctatcgcgc gacagccagc gtcaggcggc agcaagtcag tttcagcaac gaacacacgc
    26341 cgcccccgtc attacgcgag atacccaacg tcaggcagcg gcacagcggt ttaatgaagc
    26401 ggaacactat ggcaattatg acgacttccg cgagttcagc cgccaccaac cactgaccca
    26461 gcagcaaaag gacgccgctc gccagcgtta tcagtctgcc tcacctgagc agcgccaggc
    26521 ggcccgtgag aaaatgcaga ctagcccgca gaatcaacag caaagagatg cagcacgtca
    26581 gcgtattcag tccgcgtcgc ctgagcagcg ccaggtgttt aaggaaaaag tacagcagcg
    26641 cccactgaac caagagcaac gtgataacgc ccgccagcgt gttcaatcgg catcacctga
    26701 acaacgtccg ttttttcggg agaaagttca ggagagccgc ccgcaacgtc taaacgacag
    26761 taaccgtact gccaggctga ataacgagca gcggtcagca gtacgcgaac gtctctctga
    26821 acgtggggca agaagactgg aaaggtaaat tgcagacgta aaaaaagcgg cgtggttagc
    26881 cgctttttta attgccggat gttccggcaa acgaaaaatt acttcttctt cgctttcggg
    26941 ttcggcaggt cggtaatgct accttcgaac acttctgccg ccaggcccac agactcgtgc
    27001 agagtcgggt gcgcatggat ggtcagtgcg atgtcttcag cgtcacaacc catttcgatt
    27061 gccaagccga tttcacccag caactcgccg ccgttagtgc cgacaatcgc accaccgatc
    27121 acacggtgag attctttgtc gaaaatcagc ttggtcatac cgtctgcgca gtcggaagcg
    27181 atagcacgac cagaagcagc ccacgggaag gtggcggttt cgtagctgat gcctttctct
    27241 ttcgcttctt tctcagtcag acctacccat gcaacttctg gttcggtata ggcgatggac
    27301 gggataactt tcggatcgaa gtaatgtttt ttaccggcga taacttcagc ggcaacgtga
    27361 ccttcgtgaa cacctttgtg tgccagcatt ggctgaccga cgatatcgcc gatagcaaag
    27421 atgtgcggta cgttggtacg cagctgtttg tcaacgcgga tgaaaccacg gtcgtcaact
    27481 tccacgccag ctttgcctgc gtcgaggttt ttaccgttcg gcacacgacc aatcgctacc
    27541 agcacggcgt cgtaacgctg cggttcagcg ggtgcttttt tgccttccat cgtcacataa
    27601 atgccgtctt ctttcgcttc aacggcggta actttggttt ccagcatcag gttgaatttc
    27661 ttgctgatac gcttggtaaa gactttaacg atgtctttat cagctgccgg gatgacctgg
    27721 tcgaacattt caaccacgtc aatctgtgaa cccagcgcgt ggtatacggt gcccatttcc
    27781 agaccgatga taccgccacc cattaccagc aggcgttctg gtacttcttt cagttccagc
    27841 gcgtcagtgg agtcccagat acgcggatct tcatgcggaa taaacggcag ttgaatcggg
    27901 cgagaacccg ctgcaatgat cgcgttgtcg aagttgatca cggttttacc gttctcacct
    27961 tcaacttcca gggtgttagc cccggtgaat ttacccagac cgttgaccac tttgactttg
    28021 cggcctttcg ccataccagc cagaccaccg gtcagctgat tgatcacttt ctctttccag
    28081 gtacgaatct tgtcgatatc ggttttcggt tcgccgaaga cgataccgtg ttcagccagc
    28141 gctttggctt cttcgataac ttttgctacg tgcagcagtg ctttagaagg gatacagccg
    28201 acgttcaggc aaacaccgcc aagggtgttg taacgttcta cgattacggt ttccagacct
    28261 aaatcagcgc aacggaaggc agcggagtaa cctgcggggc ctgccccaag taccacgacc
    28321 tgagttttga tttcagtact catcatgacc tctatatatt tatctccggc gggtctgacg
    28381 ttttttttat gacgcccatt atccaccggg tcgttctatc cgtcggtatt ttacaaaatt
    28441 gttaacaatt tttaaacaac aaacggcaac cgatttgtct attcgctaat aacctcaata
    28501 cattcatgag attaccagaa aaaagccggc cgttgggccg gctcttttac ttacatcacc
    28561 agacggcgaa tgtcagacag cgtgttgtta atgatggtaa tgaaacgggc accatcagca
    28621 ccgtcgatca cgcggtggtc gaaggagaga gaaatcggca gcatcagacg cggcacgaac
    28681 tctttaccat tccacaccgg ctccatcgcg gacttggaaa cgccgaggat agccacttcc
    28741 ggcgcattca caatcggcgc gaagtgggta gtacccaggc cgccgatgct cgagatggtg
    28801 aagcaaccgc cctgcatttc gcccgcagtc agcttaccgt cacgcgcttt cttagaaata
    28861 gtcatcagct cgcgagacag ctcgatgatg cctttcttgt tgacgtcttt gaataccggg
    28921 acaaccagac cgttcggggt atccaccgcc acaccgatgt tgatgtattt cttcagggtc
    28981 agacgctgac cgtcttccga cagcgaactg ttgaagcgag gcatctgctc aagagctgca
    29041 gcaacggctt tcatgatgaa gacaaccggg gtgatcttca catccagctt acgtttcgct
    29101 gcttcttcgt tctgctgttt acggaacgct tccagctcag tgatatcggt tttgtcgaag
    29161 tgagtaacgt gcgggatcat cacccagtta cggctcaggt tagcaccaga gattttctga
    29221 atgcggccca gttccacttc ttcgatttca ccaaacttgc tgaagtccac tttaggccac
    29281 ggcagcatgc ctgggatacc gccgccagtt gccgccggag ctgcttctgc acgtttgatt
    29341 gcttctttca cgtaagcctg aacgtcttcg cgcaggatac gacctttacg gccagtgccc
    29401 ttcactttcg ccaggttaac gccaaactcg cgtgccagac ggcggatcag cggagtcgcg
    29461 tgaacgtaag cgtcgttttc agcaaattca gatttgcctt cggctttcgc agccggtgct
    29521 gctgccgggg cttcagcttt tgctgccgga gccggcgctg ccgcttcctg tttcgcagga
    29581 gctgccgcag gcgctgcgcc ttcaacttcg aagatcataa tcagcgaacc agttttcact
    29641 ttatcgccaa cgttgacttt cagttccttc acgacgcctg caaacggagc cggaacttcc
    29701 atagaagctt tatcgccttc tacggtgatc agtgactgtt cagcggcaac tttgtcgccc
    29761 actttcacca tcacttcggt cacttcaact tcgtcaccgc cgatatccgg aacgttaact
    29821 tctttcacgc cagctgctgg tgcagaggcc gctgccggag ccgcttcctg tttagccgcc
    29881 ggagctgccg cgccagcttc acccgcgact tcgaagacca taatcagcga gccggtagac
    29941 actttgtcac ccacgttcac tttgatctct ttcacggtac ctgcaaacgg agccggaact
    30001 tccatagaag ccttgtcgcc ttctacggtg atcagcgact gttcagcttc aactttgtcg
    30061 cccactttga ccaggatttc ggtcacttca acttcgtcgc tgccgatatc cggaacgtta
    30121 acgtcttttg ccgctgcagc cgctggtgct gctgccggag cggcttcttt cttctcttct
    30181 gcctgagcag gtgcagcgtc tgctgcaccg tcggcggaat cgaaaatcat aatcagtgcg
    30241 ccggtctggg ttttatcgcc aacagagact ttgatctctt taacgatacc cgcctgcgga
    30301 gatggtactt ccatagaggc tttgtcgcct tctacggtga tcagcgactg ttcggcttca
    30361 actttgtcgc ccactttgac caggatctcg gtgatttcaa cttcatcagc cccgatgtcc
    30421 ggtactttga tttcgatagc cattattctt ttacctctta cgccagacgc gggttaactt
    30481 tatctgcatc gatgttgaat ttggcgattg cgtcagcaac cactttctta tcgatttcgc
    30541 cacgtttagc cagttcgccc agcgccgcaa ccacgacata agaagcatca acttcaaagt
    30601 ggtgacgcag gttctcacgg ctgtcggaac gaccgaagcc atcagtaccc agtacgcggt
    30661 agtcgtcagc cggtacgtaa gtacggacct gctcagcgaa cagtttcata tagtcggtag
    30721 atgccactgc cggagcgtcg ttcatcacct gagcgatata cggaacgcgc ggagtttcca
    30781 gcgggtgcag catgttccag cgttcacaat cctgaccatc acgcgccagt tcggtgaagg
    30841 aagtcacgct ataaacgtca gaacctacgc cgtaatcttt cgccaggatc tctgccgctt
    30901 cacggacgtg acgcaggata gaaccggagc ccagcagctg aactttacct ttgctacctt
    30961 caatggtttc gagtttgtag atacctttac ggataccttc ctcagcacct tccggcattg
    31021 ccggcatgtg gtagttttcg ttcagcgtag tgatgtagta gtaaacgttc tcttgttttt
    31081 caccgtacat acgctccaga ccgtcatgca tgatgacagc aacttcgtaa gcgtaagccg
    31141 ggtcgtaaga gatacagttc gggatagtca gcgactgaat atggctgtga ccatcttcgt
    31201 gctgcagacc ttcaccgttc agggtggtac gaccggaagt accgccgatc aggaagccac
    31261 gcgcttgctg gtcgccagcc gcccagcaca gatcgccgat acgctggaaa ccgaacatcg
    31321 agtaatagat gtagaacggg atcatcggca gattgttggt gctgtaagag gtcgccgctg
    31381 ccagccagga acaacctgcg cccagctcgt tgatcccttc ctgcagaatc tgacctttct
    31441 cgtcttcttt atagtaagca acctgctcgc ggtcctgcgg ggtgtactgc tgaccgttcg
    31501 ggctgtaaat accaatctga cggaacagac cttccatacc gaaggtacgc gcttcgtcgg
    31561 cgatgatcgg taccagacga tctttgatcg acttgttctt cagcatcacg ttcagagcac
    31621 gaacgaaagc gatagtggta gagatctctt tgctctgctc ttccagcagc gcgccgaaat
    31681 cttgcaggct cggcagctcc agcttctcgg tgaagttcgg ctggcggctt ggcagataac
    31741 cgtgcagttt ctgacgttga gcgtgcagat aggtatgctc ttcagaacct tccgggaagg
    31801 tgatgtacgg cagtttttcg atatctgcat cagacaccgg cacattgaaa cggtcgcgga
    31861 tgtgacgcac gccgtccatg ttcattttct taacctggtg cgcgatgttt ttaccttcag
    31921 ccgcgtcgcc catgccgtaa cctttaatgg tatgagcaag gattaccgtc gctttgcctt
    31981 tggtttcctg cgctttcttg aatgcagcgt agattttctt cggatcgtga ccaccacggt
    32041 tcagtgccca gatctgctcg tcagtccagt ctgcaaccag tgctgcggtt tcaggatatt
    32101 taccgaagaa gtgttcgcga acatacgcac catctttcga tttgaaggtc tggtaatcgc
    32161 cgtcaacggt ttcgttcatc agctggatca gtttaccgct ggtatcttta cgcagcagtt
    32221 cgtcccaacg gctaccccac atcactttga tcacgttcca gccagcacct tcgaagatgc
    32281 cttccagttc gttgatgatc ttgccgttac cggtgaccgg gccgtcaaga cgctgcaggt
    32341 tacagttgat aacgaagacc agattatcca gtttttcacg ggtagcgatg gtgatcgcac
    32401 ctttggattc cggttcgtcc atttcaccgt cgccgaggaa cgcgtaaacg gtttgtttag
    32461 aggtatcttt caggccacgg tgttccagat atttcaggaa tttagcctgg taaatagcac
    32521 caatcggacc cagccccata gatacggtcg ggaactgcca gaattccggc atcagtttcg
    32581 ggtgcggata ggaagagagg ccattgccgt gaacttcctg acggaagtta tccagctgct
    32641 cctgagtcag acgaccttcc aggaaagcac gagcgtaaac gcctggggag atgtggccct
    32701 ggaagtaaac caggtcgccg ccatcctgct cgttgcgtgc acggaagaag tggttaaagc
    32761 acacatcata aatggttgcg gaagactgga aggacgccat gtggccgccc agttcgaggt
    32821 cttttttcga cgcacgcagc accgtcatga tggcgttcca gcggatagct gaacgaatac
    32881 ggcgttccag ttccagatta cccggatact ccggttgttc ttcaacgggg atggtgttga
    32941 tgtagttgct gataccggtg cctgcagcta cgttgactcc gcctttgcgg gcttcagcaa
    33001 gcagttggtc gatcagatac tgagcacgct caacaccttc ttcacggatg accgattcga
    33061 tcgcctggag ccagtcgcga gtttcgatcg gatccacgtc atttgggaaa cgttctgaca
    33121 tgggttattc cttatctatc taataacgtt gagttttctg gaacctgtcc cattgaactc
    33181 tcgccggaaa gctcaataag acaggttcta cgtttagttg ccgcgctttt atatgcgctt
    33241 gatttacaac atcttctgga taatttttac cagaaaaatc actaattctt tcgttgctcc
    33301 agacgacgca gagaacgctc acggcggctc tcttcacggc ttctgtcgag caaaatttct
    33361 tcgataaagg ccagatggcg atgcgatgct tcgcgcgctt cttccggctt accggccata
    33421 atcgcttcaa atatgcgggt gcggtgacta ctcaccagcg gcagcatctc gcgacgcgaa
    33481 tagagcaatt cgaagttctg gcggacattc tgagccaaca tcggctccat acaccttagc
    33541 agatgaagca gaaccacatt gtgggccgct tcggtgacgg caatctgata ctggagtacg
    33601 gcatttgatt ccgcgtccag atcgccagac tgctgcgcca gctctatggc atggtggagt
    33661 tcacggatgc gttccttgtc ttcatcggta ctacgcagcg cggcgtaata agcggcgata
    33721 ccttccaggg cgtgtcgtgt ttcgagcaag tcatactgtg attcaggatg gtcggagagc
    33781 agctccacca gcggatcgct gaagctttgc cagaggctgc tctggacaaa agtgccgcca
    33841 ccctggcgac gaagcaacaa gcccttcgct tcgaggcgtt gaatcgcctc acgcaaagag
    33901 ggacgggaga cgtcgaactg ttttgccagt tcgcgttccg gtgggagttt ttcgcccggg
    33961 cggagagtgc cttcgaggat caaaaactcc agttgctgct caatcacatc ggagagtttt
    34021 ggttggcgga ttttgctgta ggccatgagt tcctgtctta agccacttgc cgaagtcaat
    34081 tggtcttacc aatttcatgt ctgtgacgct aaagtaacaa agtattcacc ttatgtccat
    34141 acaggttttg attgaaatca tgaaactgtg cacattttaa caacttgaca tatataacgt
    34201 ttcaaagttg taactatgca caaatgttaa atttacccat caaggggtga ccttaacaga
    34261 attgaaacga aaaaatattt cctctgaacc gattcaccaa gcaacaaaac gcataacaat
    34321 tcaattatgt gaattaatat gtaaaatgaa tgttcaggga aggagagaaa ggtagtattt
    34381 acctatgttt tctttttaca ttgct
//
LOCUS       DACADF010000037        34200 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.037, whole genome shotgun sequence.
ACCESSION   DACADF010000037 DACADF010000000
VERSION     DACADF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.037"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            73..243
                     /locus_tag="ISB30_001692"
     CDS             73..243
                     /locus_tag="ISB30_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HAP1989333.1"
                     /translation="MLLKLDEETNRRLIKAKDRSRRSKTAEAYLRLKDHLERFPDFYN
                     SELAVPGGEKEE"
     gene            305..673
                     /gene="traA"
                     /locus_tag="ISB30_001693"
     CDS             305..673
                     /gene="traA"
                     /locus_tag="ISB30_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HAP1989334.1"
                     /translation="MNAVLSVQGSAVSEKTKPSFMSRVFNKKKALKVAKVALPVAAVA
                     AFFPDAAMATTTATDLMKSGDATVKGTFGKQSSVVKWVVLAEVVAGGIMYMMTKNVKF
                     LFGFAIISTFITIGMSVAGY"
     gene            687..992
                     /gene="traL"
                     /locus_tag="ISB30_001694"
     CDS             687..992
                     /gene="traL"
                     /locus_tag="ISB30_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HAP1989335.1"
                     /translation="MTGDELKRYRFPETLTNQSRWFGLYLDELIPAVICLGWGFWTSK
                     FIFGIASAVLVFWGIKKLKKGRGSSWLRDLIYWYLPTSLLKGFFHDVPDSCFRQWIK"
     gene            1012..1578
                     /gene="traE"
                     /locus_tag="ISB30_001695"
     CDS             1012..1578
                     /gene="traE"
                     /locus_tag="ISB30_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HAP1989336.1"
                     /translation="MEHGARQNTTRVIAVGFISLGTLLTLSLATNIIQGINNYRLQTE
                     QKVAVTPMLFRAPFAVSQNQADASYIEQLGLSFVALRLNVTPETVDAQHQQLLRYVLP
                     ASQNSLKVQLAEDAKRIKDNNVNSTFYMTSMRAWPAENRVDIRGELKTWIGDSKPYSE
                     IKSYVIQFSRVDGVSWLARFGEINNEKN"
     gene            1565..2305
                     /gene="traK"
                     /locus_tag="ISB30_001696"
     CDS             1565..2305
                     /gene="traK"
                     /locus_tag="ISB30_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HAP1989337.1"
                     /translation="MKKINPLFISGCLLLAAPAMSATLSGTLAPTVVPLTNGGQANIA
                     VSNTDPNLFTVPGDRITAINSLDGGLTNQEQTDSGGAILATVSKKPFTFIVETERGLN
                     FSIRAVPRAGSGRTIQLVSELAGTPGPAKAWEESNPYESLLVSLNRAVRQGSVPDEYQ
                     SVPVTSEVLQVPAGLRATADRVWVGHHLKVVRYSLDNVSLSARMVRESDFWQPGTRAV
                     MFSTPAGLLTAGGRMQIWVTTSDEGVKR"
     gene            2305..3729
                     /locus_tag="ISB30_001697"
     CDS             2305..3729
                     /locus_tag="ISB30_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraB"
                     /protein_id="HAP1989338.1"
                     /translation="MANFNTLIKRKQYIWLGLILAGGAAAVGGGLYLSDLNMSSDEEA
                     PAQGEPAPDMTGVVDSSFNSKVEQHATTEMQATAADLNKRFDSLQGEVELLSKARTAD
                     QIRIDKLSSDNEAMQNQLKSLGVKPAVSGGEPAPTPPSPPPGPEGEPQPASYPPQTGA
                     AVPPPTAFYPGNGMTPPPQVSYQSVPVPNQIQRKTFSYDKGKKTKSLPYIPSGSFAKS
                     MLIEGADANASVTGNESTVPMQLRITGRVEMPNSKTYDLTGCFVGLEAWGDVSSERAI
                     VRTRNISCIKGDKTIDQPINGHVSFMGKNGVKGEVVMRNGKILGWAWGAGFVDGIGQG
                     MERASQPAVGLGATASVGAGDVLKMGVGGGASKAAQTLSEYYIKRAEQYHPVIPIGAG
                     NEVTVVFQDGFQLKTIEELEAEKNGQQQAQNQEAEPAQQADAHHQSGGGMNGFNTDEM
                     LKKLGKLDPREFSPGAAPQGENNG"
     gene            3801..4385
                     /gene="traV"
                     /locus_tag="ISB30_001698"
     CDS             3801..4385
                     /gene="traV"
                     /locus_tag="ISB30_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HAP1989339.1"
                     /translation="MKELMLLIPLGSALLLTGCAGTETEFQCNATTSDTCMTMEQANE
                     KAKLKEERSDAKPAAAGLPQLAEGNFGTTSVNPYPLPPQPNLNRARVAAERERARAVY
                     LNNPTAKNEAIYFETGKRLQSLPVVKMSLPVVASTFTSPSTPPGNYPRPLRKGEETTS
                     LWIAPYVDSDDVYHQPATVLFVVKPSAWGQPRLN"
     gene            complement(4517..4642)
                     /locus_tag="ISB30_001699"
     CDS             complement(4517..4642)
                     /locus_tag="ISB30_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989340.1"
                     /translation="MPIATYRSVRIRLAYIFNNITTLKSILPGEKNPLSSTRPFK"
     gene            4708..4926
                     /locus_tag="ISB30_001700"
     CDS             4708..4926
                     /locus_tag="ISB30_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989341.1"
                     /translation="MHAFIFLVLMTPSSGGGIWDITPMPNTDVCIKMLRVLEARGGRG
                     YGSNTGECIEIKTREPLSSVIETLNEGG"
     gene            4927..5238
                     /locus_tag="ISB30_001701"
     CDS             4927..5238
                     /locus_tag="ISB30_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989342.1"
                     /translation="MAEYQLGFDGFVRLFFAMAIIICGYGYVIIGVFIRDTLSCILML
                     FGLTGLSALFSLMVFMDKNMWAGSPDLTLSVITGFFTATVVFYMFYVLIYYFDRMGAG
                     E"
     gene            5305..5709
                     /locus_tag="ISB30_001702"
     CDS             5305..5709
                     /locus_tag="ISB30_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989343.1"
                     /translation="MKAYYILGHNVAWLNGICLILFVIGVVGALAMVAIPEKFNLRVN
                     RGDTFIYCALIAVVGFSGMFVISIHSFSMDELEAGRHWKDDCRTLEVNMPTGAFTSPV
                     NKLDCDGIIINVPGGQYYSYIHQWELYKANSK"
     gene            complement(5696..5902)
                     /locus_tag="ISB30_001703"
     CDS             complement(5696..5902)
                     /locus_tag="ISB30_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989344.1"
                     /translation="MNNTLQLFWSSGLHIARIFNILHKKNTQLTQLKEELSKNQIAGQ
                     FLAVFPLFHPYRSIQFVNQHFILS"
     gene            6086..6484
                     /locus_tag="ISB30_001704"
     CDS             6086..6484
                     /locus_tag="ISB30_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989345.1"
                     /translation="MDMMLFLRKYGFLFIPPVYWLIYLGARLTYNGLAGEFSPDFFQG
                     IRHEGVYIICSLCVFYFFCLEVEVGDGEFSANILFLIVFFHVVLLFITLLICLWQGEV
                     LLDSIFMGQIIASMCLLFFIPVLSIIRRSF"
     gene            6556..9195
                     /gene="traC"
                     /locus_tag="ISB30_001705"
     CDS             6556..9195
                     /gene="traC"
                     /locus_tag="ISB30_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HAP1989346.1"
                     /translation="MSNNIIDAVTQTVNSLVSALKLPDESAKANDTLGSMNFPQFSRI
                     LPYKDYDSATGLFINNKTIGFMFEARPLPGADKSIVATLEHLLRSKLPRGVPVSFHLV
                     SSKLVGNDIDYGLREFRWSGKQAKKFNAITQAYYLRAAETKFPLPPALDLPLTLRNYR
                     VYISCCVPRKKNSTTQIVEMENQIKILRASLGGAYIPTRILDAAGLVELMRELINPDP
                     HEMYRVPYKLDPYQDLNYQCVDDSFDMQVTAGHLKIGRLGRDGKECVTRVTSYHLESD
                     PEMAFLWTSADNYANLLNPELSISCPFVITLTLMVEDQVKTQNEANMKFMDVEKKSKT
                     SYAKFFPNVIKEMQEWGDIRQRLATNQTSLVSYFFNITTYTADSTEASLAAEQQVLNS
                     YRKGGFQLIPARYHHLRNFLAMMPFKCGEGLFKELQAAGVVKRAETFQVANLLPIVAD
                     SPLAPAGLLAPTYRNQLAFIDLFYEGMNNTNFNMAVCGTSGAGKTGLIQPLIRSVLDS
                     GGFAWVFDMGDGYKSLCENMGGVYLDGDTLKFNPFANVLDDAHFDMSAERIRDQMSVM
                     ASPNGNLDEVHEGLLLQAVQAAWLSKRNHARVDDVVQFLQDAKDSDEYADSPTIRGRL
                     DEMIILLDQYTVNGIYGDYFNSDKPTLHDDARMVVLELGGLESRPSLLIAVMFSLIIY
                     IENRMYQSPRGLKKLNVIDEGWKLLDFKNEKVGQFIEKGYRTARRHTGAYITITQNIV
                     DFDSPTASSAARAAWGNSSYKAILKQSAKEFAKYNQLYPDQFSKLEKDMINGFGSAKE
                     QWFSSFMLQVEANCSWHRLFVDPLSRAMYSSKGPDFEYMQARRQEGVDIHDAVYGLAC
                     RNFKDEMAELESRIPVNDMEDKQ"
     gene            9195..9584
                     /gene="trbI"
                     /locus_tag="ISB30_001706"
     CDS             9195..9584
                     /gene="trbI"
                     /locus_tag="ISB30_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HAP1989347.1"
                     /translation="MKNNTEQPVSTTSLTFRRIPVIVALVLFALATSAFLSRVVLDYS
                     TPRVVAFDMKKTLDSFMDSVSQKQLTEAQSKALSDRFNDALEKSLAEYQQQHHVVILV
                     SPAVVQGAPDVTRNIQHDIARRMKGEE"
     gene            9584..10219
                     /gene="traW"
                     /locus_tag="ISB30_001707"
     CDS             9584..10219
                     /gene="traW"
                     /locus_tag="ISB30_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HAP1989348.1"
                     /translation="MIRLKDCLLAACLLSPLTQAADLGTWGDLWPVREQDMLQLITQR
                     LQSLQSSGQWGQTMDAFKQRVIENSQRPAPVEGIKRAEKYEQRWFDPSIRLTEDLKDN
                     EGRVFARKGEVVNPLKTVPFVQTLYFINGDDADQLAWMKRQVPETLMSKIILVRGSIP
                     DTSAALDSRIYFDQNGVLSKRFGLTAVPARITPAPSGERLNIETFPPVPHP"
     gene            10254..10655
                     /locus_tag="ISB30_001708"
     CDS             10254..10655
                     /locus_tag="ISB30_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989349.1"
                     /translation="MPMILALIILLTMLLLTLMWSTIRKQENVIRELSTRESWLEREV
                     ERLRTDNEKLYRWLRYNGEKVIDEMTCELDALSAVRQTNRDSYRRQLYLFDCLSLMVW
                     AGMEGAEDSTREKVRNKTDALRLVLREKTDE"
     gene            10652..11641
                     /gene="traU"
                     /locus_tag="ISB30_001709"
     CDS             10652..11641
                     /gene="traU"
                     /locus_tag="ISB30_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HAP1989350.1"
                     /translation="MKAIFFLICIAFSTFSGRAAADPSCEGRFVNPITDVCWRCIFPL
                     SLGSVQVGKGDLPDTSNPGSPLQLCPAPPPIFVRPGLAIGYWEPMAMTDVSRSPGCMV
                     NLGGFSINLGKTGMGTARKDDKQVNGAFYHVHWYKYPLTYWLNIITSAGCLEGGDMDI
                     AYLSEIDPTWVDSSLTTILNPEAILFANPIAQGACAADAMASAFHMPLDILFWCAGSQ
                     GSMYPFSGWVSNESSPLQSSLLVSERMAYKLHRQGQIMESIGKDKAVCYEYPSPIIPK
                     ERWRYQMVNMYPDSGQCHPVGRSVMRWEAGKNPPNTRKNYGYLMWRKRNCVFL"
     gene            11654..12292
                     /gene="trbC"
                     /locus_tag="ISB30_001710"
     CDS             11654..12292
                     /gene="trbC"
                     /locus_tag="ISB30_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HAP1989351.1"
                     /translation="MKLMNLFMVMLLLTGRAVLAETSSVTDREWLKQQENLSEQLRQH
                     PDKQLQQELEAQINRNPLPKSDRQFIDNLVSQQKAANQEKPAEGALYFVSFSIPEEGL
                     KRMLHETRQYGIPATLRGLVNNDMKTTTDAVLQLVKDGVTDGVQIDPTLYTQYSIRSV
                     PSLVVRCQAGYDVVRGNIHVKQALEKVAQTGDCAQVARQILDAPSNAAGSQP"
     gene            12351..14306
                     /gene="traN"
                     /locus_tag="ISB30_001711"
     CDS             12351..14306
                     /gene="traN"
                     /locus_tag="ISB30_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HAP1989352.1"
                     /translation="MKTVISVLTAHFFVLSAFIWLASPACADSGSDYKAGSDFAKQVQ
                     GNGLNSLKNFSGEQNLPGYTDNPDQTKYYGGVTASGDSSLKSDSALEFSQGDTGKTIT
                     ESFTNRPPDQISQDAPFIQAAKDTESRADSIVGDTGQSCTAQVVNRSEFTNHTCERDL
                     QVENFCTREATLKDNATTQKVNRTYQQVVTLNYARSTRQWSGNLTIPTNGRLLNASVD
                     GEPLVIPWIEECDSEGKVRDSCKSAVSESLTLFERTFPIDVISWPRSESMCSGGQNTH
                     CTKYTYDGKGKIHQSFGVDKAVIAGQNFSVSKTSRTVSSASQKPVQVTVTLVMEETET
                     VYAPEVVWVESCPFSKDEGKKTGEECISPGGTRTITLGGRDYSFTEACWKYKDTWLTQ
                     PADNGSCESLMKNTACTLSSRQCAFSSEEGTCLHEYATYSCETRTSGKQMICGGDVFC
                     LDGECDKATSGKSNDFGQAVSELAALAAAGKDVAALNGVDVRAFTGKAKFCKKFAAGF
                     SNCCKDSGWGQDVGLASCSSEEKALAKAKKDKLTVSIGEFCSKKVLGICLEKKRSYCQ
                     FDSKLAQIVQQQGRNGQLHIGFGGASSPDCRGITVAELQGIDFNKLDFTNFMDDLMKN
                     QKIPENDVLTNKTRERIKEIMSQQSAQ"
     gene            14338..14592
                     /gene="trbE"
                     /locus_tag="ISB30_001712"
     CDS             14338..14592
                     /gene="trbE"
                     /locus_tag="ISB30_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HAP1989353.1"
                     /translation="MKMSFHKKPVIDFVLRLTLTIIIISPVIYFSWDAVKGTSGSDYV
                     ESVVFILMAGFAMSVCYLFFSVLEKVINARKSKDESIYHD"
     gene            14570..14818
                     /locus_tag="ISB30_001713"
     CDS             14570..14818
                     /locus_tag="ISB30_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989354.1"
                     /translation="MNQYITIEKFIDILNEENLPQEHHVMVLAVLADISLHTDRFLIN
                     SSELVQMAAQYSPAFQKLPADRQAFISSVLSMPLFLIM"
     gene            14831..15157
                     /locus_tag="ISB30_001714"
     CDS             14831..15157
                     /locus_tag="ISB30_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989355.1"
                     /translation="MEEKKAYGLVMVFVGVFVFLLVSIMSYSLWRDRQVNAFMTTNRA
                     WGIQCDTVSQAAWVIRDGERVDLQINYLPLYCSGYRFEARDDAGKVQRQLDKYSVYQH
                     LSRQSH"
     gene            15178..15930
                     /gene="traF"
                     /locus_tag="ISB30_001715"
     CDS             15178..15930
                     /gene="traF"
                     /locus_tag="ISB30_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HAP1989356.1"
                     /translation="MRTIHAAMLAGCLCCALMPAFAKDAGWQWYNEKLKPRDEDRKAV
                     PAAPPPQMDILEKLATLQAATKRSLYEAILYPSSENFVKYFRLQNYWTQQAGLFSMSA
                     KKAMLENPELDYNLQYSHYNGTVKNQLAADYAEQRQAISTLAQHYGVMFFYRGREAID
                     GQLVQVIKNFRETYGLSVIPVSVDGVINPMLPDSRTDQGQAEQLGVKFFPAMMLVNPK
                     SGQVKPLSYGFISQDDLAKQFLYVSSDFKPNF"
     gene            15941..16180
                     /gene="traQ"
                     /locus_tag="ISB30_001716"
     CDS             15941..16180
                     /gene="traQ"
                     /locus_tag="ISB30_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HAP1989357.1"
                     /translation="MRNIRFPDLDITGMWVLAVGVFFHLIARLVRKQPELAVQAGEIF
                     GLGMVVFGGYRILNALIAEAEKEEKACEKQPDEPR"
     gene            16152..16709
                     /gene="trbB"
                     /locus_tag="ISB30_001717"
     CDS             16152..16709
                     /gene="trbB"
                     /locus_tag="ISB30_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HAP1989358.1"
                     /translation="MKNSLMNLAKTPLAALALSLALAGVAHAGTLDEVKSLWDPRGIS
                     GTETASPQSDTPQAVSGASQPRWLRLSNGKQVDLRDWKVVLFMQGHCPYCHKFDPVLK
                     QLAGQYGFSVFSYTIDGQGDDAFPEALPAPPDVMQTFFPNIPVATPTTFLVNVNTLAA
                     YPILQGATDAQGFMARVDTVFQMMH"
     gene            16755..17198
                     /gene="trbF"
                     /locus_tag="ISB30_001718"
     CDS             16755..17198
                     /gene="trbF"
                     /locus_tag="ISB30_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HAP1989359.1"
                     /translation="MNKYIRSTGLYAFLFPASLKDSGQTAAERIEQLKPEFIHRERRL
                     EIYLELFIVFLTAGALLLWIMRFLFNLCVDDWIASGDLRVKDLWNIMMYAIPYALIAV
                     GVGFFVAGVTLAIRNFFSYHLKTLFILRNDRVKKNAVRNGGQDAN"
     gene            17176..18555
                     /locus_tag="ISB30_001719"
     CDS             17176..18555
                     /locus_tag="ISB30_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HAP1989360.1"
                     /translation="MEDRMRIKLLSLFCGAALGLSFSAAADVNGDMNNFFNKLGFASN
                     TSQPQVWQGQAAGYASGGSLYARTQVKTIQLVSMTLPDINAGCGGIDAYLGSFSFINS
                     EQLQRFGKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMATDMNSMNLSSCQAAQGI
                     VGGLFPRTQVAQQKVCQDIAGESNIFSDWAASRQGCTVGGKMDSVQDKASDKDKERVM
                     KNINIMWNALSKNRLFDGNKELKEFVMTLTGTLIFGENSEITPLPARTTDQDLIKAMM
                     EGGTAKIYHCNDSDKCLKVVADATVTITANKALKNQIRALLSSIQNKAVADEKLTEQE
                     KGFISSTTIPVFKYLVDPQMLGVSNSLIYQLTDYIGYDILLQYIQELIQQARAMISTG
                     NYPQSTMDMIMENLNQASVQIAAFQSRVQVQQDALLVVDRQMSYMRQQVSARMMTRYQ
                     NNYHFGGNL"
     gene            18555..21404
                     /gene="traG"
                     /locus_tag="ISB30_001720"
     CDS             18555..21404
                     /gene="traG"
                     /locus_tag="ISB30_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HAP1989361.1"
                     /translation="MLEIYAIAGGDWLRGNLNAIAAFMGTSTWSTIEKMCIAISVLIV
                     AGNWVKKHNVMDLIGWVFSLTLVSMLVVIRTPVQIIDYSNVAQVYEVDNVPIGLAIPA
                     SLTTRVGNALIQSYEMVFALPDSVTYSKTGMLFGSNLVAKSTDFLSQNPQITTLFSDY
                     VQNCVMGDIFLNHKYSFEELLNSPDPYTLIFANPSPLRGVFDKNNQFQTCEEASRDLK
                     SALALDTQTGGKTWNYYVRQLFGGKPNPDLLFSQMIGDSYNYFYSSGQSAGQIIRQNV
                     TMNALRSGIQSYAARSGDTASLVNMANTSSLEKQRLAQATMGHQALRALPLMQTVIMG
                     LMIGMFPIMVMAAMFNMMTLQVLKGYVFALIWLQTWPLLFAILNSAMAYYAKQNGVPV
                     VLSELSQVQLKNSDIATTAGYIAVMIPPLSWGIVKSMGAAFSSAYSHFSSSGLSATSQ
                     AASGVVDGNYSFANMQMENVSGYSWGTNSTTSFGQMSRQLANGGMSTQTRDGSMVWDS
                     GGAMSKLPVDINVGRQIASAQQQMAREADVQAESALHGYNSSVTSAWNSLQQFGTNKG
                     NSTSTTTGADTTESSQDSMARSKMWNAVVANAKANNISNEESFQQLMDDSSKRTQSFD
                     AHVSGKWSSGDQLFGKLGKWGSGFSAEVGGKGSAGWGHSSGNTDNVGTSGRESNDSRH
                     DTSSQAAKDFKEASDYFTSRKTTTSGNITDNNASSRVDQFAASLSSAKNSYDQYTSSR
                     TRSHEYSEMASRTESMTGQMNENLTQQFANFVQHRAPQDAEAILTNTSSPEIAAQREA
                     LAREFVKEQVEPRVDAAYQQGRESIGQNMAGVSGGGDNGSVMADYGQNSRRIDVMTQD
                     AGIKDNVGQKVDRMITENKQQHQETRENIQRQGAEVKSENAEMEKNHKTKANEFKGDY
                     NERKSKVNSLPGADSQAELEEKAAKTQKDFLDGKR"
     gene            21407..21940
                     /locus_tag="ISB30_001721"
     CDS             21407..21940
                     /locus_tag="ISB30_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraS"
                     /protein_id="HAP1989362.1"
                     /translation="MMMTNKILIEEVDELKNVFSRGEMEIPSFWSCLWPGVIVAAWNM
                     ICALISVGGGYLSVKFIFWVLVFPGVISLIVLLGVASARSLFLSVPRTYRMRSQVYRF
                     ISRKVMVYALVYMALILALALFNRVYYASPFPFSFMILIATIFVWFVMNIDFGRYQLS
                     VLSSAINSFRAEKLGGG"
     gene            22126..22857
                     /gene="traT"
                     /locus_tag="ISB30_001722"
     CDS             22126..22857
                     /gene="traT"
                     /locus_tag="ISB30_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HAP1989363.1"
                     /translation="MKLNKFMAVMVVSSALVLSGCSAMGTAIKKRNLEVKTQMSETIW
                     LEPSNNKTVYLQIKNTSDKDMSGLQAKIASAVTSKGYQVVSNPDTAGYWIQANVLKAD
                     KMDLRESQGWLSRGYEGAVTGAALGAGITAYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKTQVQTDNVAVLRQGTSGAKVQTSTETGNQHKYQTRVVSNAN
                     KVNLKFPEAQPVLEDQLAKSIANIL"
     gene            23050..23739
                     /locus_tag="ISB30_001723"
     CDS             23050..23739
                     /locus_tag="ISB30_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989364.1"
                     /translation="MTISVPVRSLDTHELMPGDIYPHPAAGGIDSLSHREVCSLFSDH
                     IMYICSALVILLLVFIAGVFFRKIMTSSVERKRNTASVISDAKPEVQSKPEYFHLSIC
                     VEGPASTDDSALSSLASRVVREAENLYIPGVNRRDSADDVFAWSVQTQFPSDRLFGTR
                     IFPSTGWFERPDMIKHPVKCYCVCLQYTTETLPSTLYALVDEAGRQVAKSYIKGGHCP
                     GSEGYTFCITS"
     gene            23866..26178
                     /gene="traD"
                     /locus_tag="ISB30_001724"
                     /pseudo
     CDS             23866..26178
                     /gene="traD"
                     /locus_tag="ISB30_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229688.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
     gene            26178..31439
                     /gene="traI"
                     /locus_tag="ISB30_001725"
     CDS             26178..31439
                     /gene="traI"
                     /locus_tag="ISB30_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAP1989365.1"
                     /translation="MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGID
                     GKAVDKALFTELLKGKLPDGSDLTRIQDGANKHRPGYDLTFSAPKSVSVMAMLGGDKR
                     LIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNLIVAKFNHDTNRNQEPQIHTH
                     AVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVEKLGYETEV
                     VGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKDAIDPAEKMV
                     EWMNTLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKV
                     QFTYADLLARTVGQLEAKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDEL
                     AVKALSQEVQRQNHVSVTPDAFVVRQVPFSDAVSVLAQDRPVMGIVSGQGGATGQRER
                     VAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTGKSALQDGTAFIPGGTLI
                     VDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYRWQGGH
                     QTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEG
                     VLGEKDTTITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTGSSNM
                     LTLKDREGVRLDLKVSAVDSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVM
                     KVEDGQLTVQRPGQKTTQTLAMGAGVFDGIKIGHGWVESPGRSVSETATVFASVTQRE
                     LDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQLKTRSGETDLDAAIA
                     QQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVSMADIDTTIQAQI
                     RSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLT
                     AGQRAATRMILESTDRFTVVQGYAGVGKTTQLRAVMSAISLLPEETRPRVIGLAPTHR
                     AVGEMQSAGVDARTTASFLHDTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSL
                     IVAGGGRAVSSGDNDQLQPIAPGQPFRLTQQRSAADVAIMKEIVRQMPELRPAVYSLI
                     ERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPKQEKAIEKALSEGKTLPEGQP
                     ATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLPV
                     LVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKANQLITLTDSEGKERYISP
                     REASAEGVTLYRQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKL
                     TRTLTPKADQAQQHIDLAYAITAHGAQGASEPYAIALEGVAGGREQMASFESAYVALS
                     RMKQHVQVYTDNREGWIKAIKHSPEKATAHDILEPRNDRAVKTADLLFGRARPLDETA
                     AGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIWLSPLTDRDGRL
                     EAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
                     PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAR
                     EVAGEARKAGEPADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASE
                     RLQRERLQAVERDMVRDLNREKTLGGD"
     gene            31520..32041
                     /gene="traX"
                     /locus_tag="ISB30_001726"
                     /pseudo
     CDS             31520..32041
                     /gene="traX"
                     /locus_tag="ISB30_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
     gene            32235..33776
                     /locus_tag="ISB30_001727"
     CDS             32235..33776
                     /locus_tag="ISB30_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HAP1989366.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            33791..>34200
                     /locus_tag="ISB30_001728"
     CDS             33791..>34200
                     /locus_tag="ISB30_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP1989367.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKAL"
BASE COUNT         8390 a         8486 c         9692 g         7632 t
ORIGIN      
        1 tggtaatctt actttggagg tcgattggaa agagcgatta ggagcaacgc acgacctgcg
       61 cctggaaaaa atgtgctttt aaaattagat gaagaaacta atcgtcgact catcaaagca
      121 aaagacagaa gcagaagaag taaaacagct gaagcttatt tgagactgaa agatcatcta
      181 gaacgttttc cggactttta taactcggaa ctagccgtac caggaggtga aaaggaagag
      241 tgatttaaca tcatagtcaa aaatctgttt tatatatcaa ccattctttt taaaggaaat
      301 aaatatgaat gctgtattaa gtgttcaggg ctctgctgtc tctgaaaaaa ccaaaccgtc
      361 ttttatgtcc cgcgttttca ataaaaagaa agccctgaaa gtcgctaaag ttgcgctgcc
      421 ggttgctgct gtggcggcgt tcttcccgga tgctgcgatg gccaccacca cggcaaccga
      481 cctgatgaag tccggggatg cgaccgttaa aggcacgttt ggtaagcagt ccagcgtcgt
      541 caaatgggtg gttctggcag aagttgtcgc cggcggcatc atgtacatga tgaccaaaaa
      601 cgtgaagttc ctgtttggct tcgcgattat ttccaccttt atcaccatcg gtatgtcggt
      661 cgccggttac tgacaggaag cacgtgatga cgggagacga attaaagcga taccgtttcc
      721 cggaaacact gactaaccag tcccgctggt ttggcttata tctcgatgaa cttatcccgg
      781 ccgtgatttg tctcggctgg ggtttctgga ccagcaagtt catttttggc atagcttcag
      841 cggtactggt tttctggggt atcaagaaac tgaaaaaagg gcggggcagt tcctggctac
      901 gcgacctgat ttactggtat ctgccaacct cccttcttaa aggtttcttt catgatgttc
      961 cggactcctg tttccggcag tggattaaat aaatcaacgg ggaaataaca tatggaacat
     1021 ggtgccagac aaaataccac ccgcgttatc gctgtgggat ttatcagtct ggggacactc
     1081 ctgaccctca gtcttgcgac caatattatc cagggcataa ataattaccg tctgcagacg
     1141 gaacagaaag tggcggtgac accgatgtta ttccgtgcac cctttgcggt gtctcagaac
     1201 caggctgacg cctcttatat tgagcagctg ggcctgtcct ttgttgccct gcgtctgaat
     1261 gtcacgccgg aaacggttga tgcccagcat cagcagctac tccggtatgt attacccgcc
     1321 tcgcagaaca gcctcaaggt ccagctggct gaagatgcga aacgcattaa agacaataac
     1381 gttaattcca ccttttatat gacgtcgatg cgtgcctggc cggcagagaa ccgcgtggat
     1441 atccggggtg agctgaaaac gtggattggg gattccaaac cctacagtga aattaaaagc
     1501 tatgtcattc agttcagtcg tgtcgatggt gtcagctggc tggcacgttt tggagaaata
     1561 aataatgaaa aaaattaacc cgttatttat ttcaggctgt ctgctgttgg ccgcccccgc
     1621 gatgtccgcg accctcagcg gtacgctggc gccgacagtg gtccccctaa ccaatggtgg
     1681 tcaggcaaat attgccgtga gtaacaccga cccgaatctg ttcacagtac ctggcgatcg
     1741 catcacggcg ataaacagcc tggacggtgg cctgacaaac caggagcaga ccgacagcgg
     1801 cggtgccatt ctggcgaccg tcagtaaaaa accattcacc ttcattgtgg aaaccgaacg
     1861 cgggctgaac ttctccattc gtgccgtgcc ccgtgcgggc tcaggccgga caattcagct
     1921 ggtcagcgag ctggcgggta cccccggtcc ggcaaaagcc tgggaagagt cgaaccccta
     1981 tgaatcgtta ctggtttcgc tgaatcgcgc cgtgcgtcag ggcagcgtac ccgatgagta
     2041 tcagtctgtc cccgtcactt ctgaagtgct gcaggtgccg gctggtctgc gcgccactgc
     2101 cgatcgggtc tgggtcggtc atcacctgaa agttgtccgt tacagtctgg acaatgtctc
     2161 cctgtcggcc cgtatggtgc gtgaaagcga tttctggcag cccggaacac gtgccgtcat
     2221 gttcagtacc ccggcgggcc tgctgactgc cggtggtcgc atgcagatct gggtcacgac
     2281 gtcagatgaa ggagtgaagc gctgatggcc aacttcaaca cgctgatcaa acgtaagcaa
     2341 tatatctggc tgggactcat tctggcgggc ggcgcggcag cagtcggagg cgggctgtat
     2401 ctgtcagatc tgaacatgag ctctgatgag gaagccccgg ctcagggcga acccgcaccg
     2461 gatatgaccg gggtggtcga cagcagcttc aacagtaagg tggagcagca cgcgacaaca
     2521 gaaatgcagg cgactgccgc tgatctcaac aagcgttttg actccctgca gggggaagtg
     2581 gaactactga gtaaggcgcg aaccgctgac cagatacgta tcgacaaact cagcagtgac
     2641 aatgaagcca tgcagaatca gctgaagtcg ctgggcgtga aaccggctgt ctctggtggc
     2701 gagcccgcgc cgactcctcc gtcaccgcca cccggtccgg aaggggagcc gcagccagcc
     2761 agttatccgc cgcaaaccgg cgccgcggta ccgcctccga cggcattcta tccggggaat
     2821 ggtatgacac cgccgccgca ggtcagctac cagtctgtac cggtaccaaa ccagatacag
     2881 cgtaaaacgt tcagttacga caaaggaaaa aaaacgaagt cattgccgta cattccgtcg
     2941 gggagctttg caaaaagcat gcttatcgag ggggcagatg ccaacgcgtc tgttaccggg
     3001 aatgaatcaa ctgtaccgat gcagttgcgt atcaccggtc gcgtggaaat gccaaacagc
     3061 aaaacctacg acctgacagg gtgttttgtg ggccttgaag cctggggcga tgtctccagc
     3121 gaacgcgcca ttgtccgtac ccgcaatatc agctgtatca aaggtgataa aaccatcgat
     3181 cagcccatta acggccacgt ttcctttatg gggaaaaacg gggttaaggg agaggtggtg
     3241 atgcgtaacg gaaaaatcct cggctgggca tggggggccg gttttgttga cgggattggt
     3301 cagggcatgg aacgggcttc acagccggct gttggcctgg gggcaaccgc atctgttggc
     3361 gccggtgacg ttctgaaaat gggggtcggc ggtggtgcat ctaaagctgc gcagacgctg
     3421 agcgagtact acatcaaaag ggccgagcag tatcacccgg tcattccgat tggtgccggc
     3481 aacgaagtga ccgtcgtgtt ccaggatgga ttccagctga aaacgattga agaactggaa
     3541 gcggaaaaga acgggcaaca gcaggctcag aatcaggagg cggagccggc acagcaggct
     3601 gatgcacatc atcagtcagg cggcggtatg aacggattca atactgacga gatgctgaag
     3661 aagctgggca aacttgatcc gcgtgagttc tctccgggcg cagcgccgca gggggaaaat
     3721 aatggctagt tcgggcgatt aaggaaaata cccagctcag gatgaacctg aaaaaaaacc
     3781 gataaatagc gagaaatatt atgaaggaat taatgctgtt aattcctctg ggcagcgctt
     3841 tgctcttaac cgggtgcgcg ggcacagaga ctgaatttca atgtaatgcc acgacgtcgg
     3901 atacctgtat gacgatggag caggcaaatg aaaaagcgaa gctgaaggag gaacgcagtg
     3961 atgcaaagcc ggctgcggcc gggttgccac aactggctga gggtaatttc ggaaccacgt
     4021 cagttaatcc gtacccgttg ccgcctcaac ccaacctgaa ccgggccaga gtggcagcgg
     4081 agagagaacg agcacgtgca gtgtacctaa ataatccaac ggcaaagaat gaggcaatat
     4141 atttcgaaac cgggaaaagg ctccagtctt tgccggtagt gaaaatgtct ttaccagtag
     4201 tggcgtcaac gtttacgtca ccgtcaacac cgccaggtaa ttatccccgg ccattaagaa
     4261 aaggggagga gacaacgtca ttgtggatag cgccgtatgt ggattcagat gatgtatatc
     4321 accagccggc aacggttctt tttgtcgtaa aaccatcggc gtggggacaa cctcgcctta
     4381 attgattcac agcgccatta ctggcgctgc agtaagtttt catttctgaa ttataacgag
     4441 tgccctctgc gttgaaaggg atgggcggta gcgtgttcag acgaagatat tacgctcatg
     4501 tatctttatg taagggttat ttaaaagggc gggttgatga taaagggttt ttctctccag
     4561 gcaaaatcga cttcagtgta gtgatgttat tgaaaatgta agcaagtctg attctgacag
     4621 agcgataagt ggcgataggc aaacaactat cgcagaggca atgaaagaaa tattcattcg
     4681 ttaatatata aacagaggca ataaagtatg catgccttta tttttctggt gctgatgacg
     4741 ccgagtagtg gcggtgggat ttgggatatt actcccatgc ctaatacaga tgtctgcata
     4801 aaaatgctac gggtactcga agcacgtggg ggcagaggtt acggaagtaa tacaggagag
     4861 tgcattgaaa ttaaaacccg cgagcctttg agcagcgtga tagaaacatt gaatgagggt
     4921 gggtaaatgg ctgaatacca gttaggtttt gatggttttg ttcgactttt ttttgcaatg
     4981 gcaatcatta tctgcggtta cggctatgta attattggtg tttttataag agatacgctt
     5041 tcctgtattt taatgttgtt tgggcttaca ggtctcagtg ctttattttc attaatggta
     5101 ttcatggata aaaacatgtg ggccggctct cctgatttga cgctgtcagt aataacggga
     5161 ttttttacag ccacagtggt tttttatatg ttttatgtct tgatttatta ctttgatcgg
     5221 atgggggcag gtgaatagta agaccgatgg tcagtattaa caatctcacg ttatttgaaa
     5281 gtatgcagca taaggaataa gaaagtgaaa gcgtactata tacttggaca taacgttgca
     5341 tggcttaacg gtatatgcct gattcttttt gttattggtg tggtcggtgc gctggcaatg
     5401 gttgccatcc ctgaaaaatt caatcttcga gttaacagag gcgatacatt catatactgt
     5461 gcgcttatag cggtggttgg attttccggg atgttcgtta tctccattca ctcattttcg
     5521 atggatgaac ttgaagcagg tagacactgg aaagatgact gccggacact tgaggtaaac
     5581 atgccgacag gcgcttttac cagccctgtt aataaactgg actgtgatgg catcatcatt
     5641 aatgtgcctg gtgggcagta ttattcctat atccatcagt gggaattata taaagctaac
     5701 tcaaaatgaa atgctgatta acaaactgga ttgaccggta gggatggaac aatggaaaga
     5761 cagcaagaaa ttggccagca atttggtttt ttgatagttc ttcttttaat tgtgttaact
     5821 gggtattttt tttgtgcagt atattgaaga ttctagcgat atgcagcccg ctactccaga
     5881 acaactgcaa agtattattc attagacacc ttgtgcaggt gaggcgttac gtttagcaat
     5941 tcaaactaac acaggcactt cgtcagagcc attaactttc ggtgacgcaa aaaagatggc
     6001 atcagaatgc aagtataaag aagagattaa agcagtacgt gaaaaaaact taacgcactg
     6061 aacgctatga gcaaataatc aggggatgga tatgatgctg tttcttcgaa agtacggatt
     6121 tttgtttata cctccagtct actggctcat ctatctggga gccagactga cttacaatgg
     6181 actggcagga gagttttctc ctgatttctt tcagggtatt cgacatgaag gcgtctatat
     6241 tatctgtagt ttgtgtgtat tttatttttt ctgcttagag gtggaagttg gtgatgggga
     6301 gttctcagcg aacattctgt tcctgattgt tttctttcat gtggttttat tatttatcac
     6361 tctgcttatt tgtctgtggc aaggggaggt gttgttggat tcgatcttta tgggtcagat
     6421 tattgcttcc atgtgtttat tgttttttat tccagtgctt tccattataa gaaggagttt
     6481 ctagtgcgga tatgaatatt cttcttgcca tttattaccc ttgttatttt gaaatgctct
     6541 taaaggaacg cctgcatgag taataacatc atcgatgccg tcacccagac tgtgaattca
     6601 ctggtatcag cactgaagct gcccgacgaa tccgcgaaag ccaatgacac gctgggcagc
     6661 atgaatttcc cccagttcag ccgtattttg ccctataagg attatgattc cgcgaccggg
     6721 ctgtttatta ataacaaaac catcggtttt atgtttgagg cgcgtccttt acccggtgcg
     6781 gataaaagta tcgtcgccac actggagcat ttactgcgca gcaaacttcc ccggggcgtc
     6841 ccggtcagtt ttcatctggt ctccagcaaa ctggtcggca atgatatcga ctacggtctg
     6901 cgtgaattcc gctggagcgg taaacaggct aagaagttta acgccatcac ccaggcctac
     6961 tatctccggg cagcggagac gaagttcccc ctgcctcctg cactggattt acccctgacg
     7021 ctgcgtaact accgcgtgta catctcctgc tgcgtgcccc ggaagaaaaa ctccaccacg
     7081 caaattgtgg agatggagaa ccagattaaa atcctgcggg cgtcactggg cggtgcctat
     7141 attccgacgc gtatccttga tgctgccggg ctggtcgaac tgatgcgcga gctgataaac
     7201 ccggacccgc acgagatgta ccgcgtaccg tataagctgg acccctatca ggatctgaac
     7261 tatcagtgtg tcgatgacag ctttgatatg caggtcaccg ccggtcatct gaaaattggc
     7321 cgcctggggc gcgacgggaa agagtgcgta acccgggtga cgagctacca tctggagagt
     7381 gatccggaaa tggccttcct ctggacctcc gcggataact acgccaacct gcttaacccg
     7441 gagctctcca tctcctgccc ctttgtcatc acgctgacgc tgatggtgga ggaccaggtg
     7501 aagacccaga atgaagccaa catgaaattc atggacgtgg agaagaaaag caaaacctcc
     7561 tacgcgaagt tttttccgaa cgtgataaag gaaatgcagg agtggggcga tatccgccag
     7621 cgactggcga ccaaccagac ctccctggtg tcctacttct ttaatatcac cacgtacacc
     7681 gcagacagca cagaggcctc tctcgccgct gaacagcagg tgctcaacag ctaccgtaaa
     7741 ggcggtttcc agctgatccc ggcccgctac catcacctgc gtaactttct ggccatgatg
     7801 ccgttcaagt gtggtgaggg cctgtttaaa gagctgcagg cggcaggcgt ggtgaagcgg
     7861 gcggagacgt tccaggtggc taacctcctg ccgatcgtcg ctgacagccc gctggcgccg
     7921 gcgggactcc tggcgcccac ctaccgaaat cagctggcgt tcatcgacct gttctacgag
     7981 ggaatgaaca ataccaattt caacatggca gtctgcggga cgtccggggc gggtaaaacc
     8041 ggtctgattc agcccctcat tcgcagtgtg ctcgactccg gcgggtttgc ctgggtgttc
     8101 gacatgggcg atggctataa gtccctgtgt gaaaacatgg ggggcgtata cctcgatggc
     8161 gataccctca agtttaaccc gttcgccaac gtgctggatg atgctcactt tgacatgtca
     8221 gcagaacgta tacgcgatca gatgtcggtg atggccagtc cgaacggcaa cctcgatgaa
     8281 gtccatgaag gtctgcttct gcaggcagtt caggcggcct ggttaagcaa gcgtaatcat
     8341 gcacgcgttg atgacgtagt tcagttttta caggacgcga aggacagcga tgaatatgct
     8401 gattccccga caatccgggg ccgtctggac gagatgataa tcctgctcga tcagtacacg
     8461 gtgaatggga tctacgggga ctatttcaac tcagataaac caacacttca cgatgacgcc
     8521 cggatggtgg tactggagct cggtggcctg gagtcgcggc cctctctgct tatcgcggtg
     8581 atgttctcat tgattattta catcgagaac aggatgtatc agtcaccgcg tggcctgaaa
     8641 aaactgaacg tcatcgatga gggctggaag ctccttgatt tcaagaatga aaaagtgggg
     8701 cagtttatcg agaaaggcta ccgtaccgcg cgtcgacata ccggcgcata catcaccatc
     8761 acgcaaaata tcgtggactt cgactccccg accgcatcga gcgctgcacg cgctgcctgg
     8821 gggaactcgt cgtacaaggc cattctgaag cagtcagcca aagagtttgc caagtataac
     8881 cagctgtacc cggatcagtt cagcaagctg gagaaagaca tgattaacgg cttcggttcg
     8941 gccaaagagc agtggttctc ctcttttatg ctgcaggtgg aagcgaactg ctcctggcac
     9001 cgcctgttcg ttgacccgct cagccgggcg atgtactcct ccaaaggccc ggattttgag
     9061 tacatgcaag cgcgccggca ggagggggtc gatatccacg atgcggtata cggactggcg
     9121 tgccgcaact ttaaagacga aatggcagag cttgaatccc gaataccggt gaatgacatg
     9181 gaggataaac agtgatgaaa aacaacaccg aacaaccggt cagtacgacc tctcttacct
     9241 ttcgccggat acctgtcatc gttgctctcg tgcttttcgc cctggcaacc agcgcgtttc
     9301 tctcacgcgt ggttctggac tactccacgc cgcgggtggt ggcttttgat atgaagaaaa
     9361 cgctcgacag ctttatggac agtgtgagtc agaagcagct gacagaggcg cagtccaaag
     9421 cgctctctga tcggtttaac gacgcgctgg agaagagtct ggcggagtat cagcagcagc
     9481 atcatgtggt cattctggtt tcacctgccg tggttcaggg cgcgcctgat gtgacccgca
     9541 acatccagca cgacattgcc cgccggatga aaggagaaga gtaatgatac gactgaaaga
     9601 ctgtctgctc gccgcctgcc tgttatcgcc gctgactcag gcggctgatc tcggtacctg
     9661 gggggattta tggcccgtcc gggagcagga catgctgcag ctcatcacgc agcgtctgca
     9721 gtcgctgcaa tcctctggcc agtggggcca gacgatggat gcgtttaagc agcgggttat
     9781 cgagaacagt cagcgtccgg cgccggtgga aggcattaag cgtgcggaga aatacgagca
     9841 gcgctggttt gatcccagca tccgactgac cgaagacctg aaggacaatg aggggcgggt
     9901 gtttgcccgc aagggcgaag tggtgaaccc gcttaaaacc gtaccgtttg tccagaccct
     9961 gtacttcatc aatggtgacg atgccgacca gctggcctgg atgaagcgtc aggtgccgga
    10021 aaccctgatg agcaaaatta tcctggtgcg tggcagtatc ccggacacct ccgcagcgct
    10081 ggacagccgg atctatttcg accagaacgg tgtactgagc aagcgatttg gcctgacggc
    10141 ggtcccggcg cggatcacgc cggcgccctc cggtgaacgg ctgaatattg agaccttccc
    10201 gcccgtcccg cacccctgat ctggggctgc gttttattca aaagaggtaa ccgatgccga
    10261 tgatactggc cttaataatc ctgctcacga tgctcttgct gacgctaatg tggtccacca
    10321 tccgtaagca ggagaatgtc atacgggaac tcagtacaag agaaagctgg ctggagagag
    10381 aggttgagcg tctcaggacc gataacgaga agctgtaccg ctggctgaga tacaacggtg
    10441 aaaaggttat cgatgaaatg acttgtgaac tcgatgcact gtccgccgtt cgccagacga
    10501 acagggattc ataccgtcgc cagctttacc tcttcgactg cctgtccctg atggtgtggg
    10561 ccgggatgga gggcgccgaa gactctacac gggaaaaggt ccgcaataaa accgatgccc
    10621 tccgtcttgt tctcagggag aaaactgacg aatgaaagcc atattttttt taatctgcat
    10681 cgcgttctca acgttcagtg gccgcgccgc cgcagacccc tcctgtgagg gccggtttgt
    10741 taacccgatc accgatgtgt gctggcgctg catatttcct ttatcactcg gcagcgtgca
    10801 ggttggtaaa ggcgatctac ctgataccag caatccgggt agtcccctgc agctgtgtcc
    10861 ggcgccgcca ccgatttttg ttcgtcccgg cctggccatc gggtactggg agccgatggc
    10921 catgacggac gtcagccgtt cgcctggctg tatggtcaat ctggggggct tcagtatcaa
    10981 tctcggcaag accggtatgg ggaccgcgag aaaggacgat aagcaggtga acggcgcttt
    11041 ttatcacgtt cactggtaca agtacccgct gacctactgg ctgaacatca tcacctccgc
    11101 aggctgtctg gaaggcggag acatggatat agcctatctg tccgagattg acccgacctg
    11161 ggtggacagc agcctcacca ccattctgaa tccggaagcc atcctgtttg ccaaccccat
    11221 cgcacagggg gcgtgcgcgg ctgacgctat ggccagcgcg tttcatatgc cactcgatat
    11281 tctgttctgg tgtgccggct ctcagggttc gatgtacccg tttagcggct gggtaagcaa
    11341 cgagtccagt ccgctgcagt cctcgttact tgtcagcgag cgcatggcgt acaagcttca
    11401 ccggcaggga caaattatgg agagcatcgg gaaggacaag gcggtctgct atgagtatcc
    11461 gtccccgatc atccccaaag agcgctggcg ctatcagatg gtgaacatgt acccggacag
    11521 cggccagtgc catcccgtgg ggcgcagcgt gatgcgctgg gaggccggta aaaatccacc
    11581 caacacccgt aagaactatg gctatctgat gtggcgtaaa cgcaattgtg tctttctctg
    11641 acaggagtct ctcatgaaat taatgaatct gtttatggtc atgcttttgc tgaccggacg
    11701 agcagtgttg gctgaaacct cctctgttac agacagagaa tggctgaagc agcaggagaa
    11761 cctcagcgaa cagctgcgcc agcatccgga taaacagctg cagcaggagc ttgaggcgca
    11821 aatcaaccgt aatcctctgc ccaaatcaga ccgccagttt atcgataatc tggtcagcca
    11881 gcagaaagcg gcgaatcagg aaaagccggc agaaggggcg ctgtactttg tctccttctc
    11941 gatacctgaa gaggggctga agcggatgct gcacgaaacc cggcagtacg gcattccggc
    12001 gacactgcgt gggctcgtca ataacgacat gaagaccacc actgacgcgg tgctgcagct
    12061 ggtgaaggat ggcgtgaccg acggcgtgca gatcgacccg acactatata cgcagtacag
    12121 catccggagt gtgccgtctc tggtggtacg gtgtcaggcc ggatatgacg tggtacgcgg
    12181 taacattcac gtcaaacagg cgctggagaa agtggcacaa accggtgact gcgcacaggt
    12241 ggcccggcaa atacttgatg cgccctcgaa tgccgccggg agccagccat gaaagataaa
    12301 ctgtacttca tggcgttacc ggaaatggtg agggatgagg gagggtgtaa atgaagacgg
    12361 ttatttccgt gctgaccgcg catttttttg tcctgagcgc cttcatctgg ctggcatccc
    12421 ccgcctgcgc tgacagtgga agtgactata aagccggttc ggattttgcg aaacaggtgc
    12481 agggcaatgg tctgaactca ctaaaaaatt tcagtggtga gcaaaacctg ccggggtata
    12541 ccgacaaccc ggatcagaca aaatactacg gcggcgtcac tgccagcgga gacagctctc
    12601 tgaaaagcga ttcagccctg gagttcagcc agggagatac cggcaaaacg attacggagt
    12661 cgttcacgaa ccggccaccg gatcagatat ctcaggatgc gccgtttatt caggccgcaa
    12721 aggatacaga atcccgtgcc gacagcattg tcggtgatac cggacagtcc tgtaccgccc
    12781 aggttgtgaa ccgcagcgag ttcacaaacc atacctgcga acgtgatctg caggtggaaa
    12841 atttttgcac acgggaagcg accctgaagg ataacgccac cacacagaaa gttaaccgaa
    12901 cctatcagca ggtggtgacg ctgaattatg cccggagtac ccggcaatgg agtggcaacc
    12961 tgacgatccc gaccaacggt cgtctgctga atgcctcggt ggatggtgag cctctggtga
    13021 tcccctggat tgaagaatgc gattccgaag ggaaggtgcg ggatagctgc aagagtgcag
    13081 tgagtgaaag cctgaccctc tttgaacgaa cgttccccat tgatgtgatc agctggccac
    13141 gttcagaatc gatgtgttca ggcggtcaga acacgcattg tacgaaatat acctatgacg
    13201 ggaaggggaa gattcatcag tcgtttggtg tggacaaagc ggtcattgct gggcagaact
    13261 tttcagtaag caaaacatca cggaccgtat catcggccag ccagaaaccc gtacaggtca
    13321 cggtcaccct ggtgatggaa gaaaccgaaa ccgtctatgc acctgaagtc gtctgggttg
    13381 aaagctgtcc gttcagtaag gatgaaggga aaaaaacggg cgaggaatgt atcagtccgg
    13441 gaggaacgcg aaccatcacc ctcggaggca gggattactc gttcacggag gcctgctgga
    13501 aatataagga tacctggctc acgcagccgg cggacaacgg ctcctgtgaa agcctgatga
    13561 aaaatacggc ctgtactctg tcatcccgcc agtgcgcctt ctccagcgag gaaggcacgt
    13621 gcctgcatga atatgcaacg tattcctgcg aaaccaggac gagtggcaag caaatgatct
    13681 gcggcggtga tgtcttctgc cttgatggtg aatgtgacaa agccacgtcc gggaagagca
    13741 acgactttgg gcaggcggtg tctgagctgg cggcactggc tgccgcgggg aaggacgtcg
    13801 cggcactgaa cggggtggat gtccgggcat tcaccggaaa agcgaagttc tgtaagaagt
    13861 ttgctgccgg cttcagtaac tgctgcaaag acagtggctg gggccaggat gtggggctgg
    13921 caagctgcag cagcgaggaa aaggcgctgg ccaaagcgaa aaaagacaag ctgacggtca
    13981 gtatagggga attctgctct aaaaaggttc tggggatttg cctggagaaa aaaaggagct
    14041 attgccagtt cgactcaaaa ctggcgcaga tcgttcagca gcagggaaga aacggtcagc
    14101 tgcatatcgg ttttggcggc gccagcagtc ctgactgtcg gggcattacc gtcgcggaac
    14161 tccaggggat tgattttaac aagctggatt tcaccaactt tatggacgac ctgatgaaaa
    14221 atcaaaaaat accggaaaac gatgtgctca ccaataagac cagagaacga attaaggaaa
    14281 tcatgagtca gcaatccgcg caataaacca tgctgcctaa taaagagacc gataatcatg
    14341 aaaatgagtt ttcacaaaaa gcctgttatt gattttgtcc tgagattaac gctaacaata
    14401 atcattattt ctccggtgat ttatttctcc tgggatgcag tcaaagggac gtcgggaagt
    14461 gattatgtcg aaagtgtggt ctttattctt atggctgggt ttgccatgtc tgtatgttat
    14521 ttgtttttct cagtcctgga gaaagttata aatgcaagga aaagcaaaga tgaatcaata
    14581 tatcacgatt gagaaattta tagacattct gaatgaagag aatttacccc aggagcatca
    14641 tgttatggtg ctggctgttc ttgcggatat ctctctgcat acagacaggt tcctgattaa
    14701 ctcgtccgaa ctggttcaga tggccgctca gtactcacct gctttccaga aactgccagc
    14761 ggaccggcag gcttttatat cttcagtact gagcatgccg ctattcctta ttatgtgacc
    14821 aggagagtca atggaagaga aaaaagcata cgggctggtc atggtgtttg tcggggtgtt
    14881 tgtctttctc ctggtcagca ttatgtcgta cagcctgtgg cgggacaggc aggttaacgc
    14941 atttatgaca accaaccggg cctgggggat ccagtgcgac actgtttctc aggccgcgtg
    15001 ggtgatcagg gacggggagc gggtggacct tcagataaat tatctgcctc tgtactgcag
    15061 cggttatcgc tttgaagccc gtgatgatgc aggaaaggta cagcgccagc tcgataaata
    15121 cagtgtctat cagcatctgt cacgccagtc gcattaattc ataaaagggt atttgccatg
    15181 cgaacaatac atgccgccat gctggcgggg tgcctttgct gcgccctgat gccggctttc
    15241 gccaaagacg cgggctggca gtggtacaac gagaaactga agccccgtga tgaggacagg
    15301 aaagccgtac cagcggcacc accgccgcaa atggatattc tggagaaact cgccacactc
    15361 caggcggcca ccaaacggtc gttgtacgaa gccattctgt atccatcgtc tgaaaacttc
    15421 gtgaagtatt tccggctgca gaactactgg acgcagcagg caggtttgtt ctcgatgagt
    15481 gcgaagaaag ccatgctgga aaaccccgaa ctcgattaca acctgcagta cagccactac
    15541 aacggcacgg taaaaaacca gctcgctgcc gactatgccg aacagcgaca ggctatctcc
    15601 accctcgcac agcactacgg cgtgatgttc ttctaccggg gccgggaagc gatcgatggc
    15661 cagctggtcc aggtgattaa aaattttcgc gaaacctatg gcctgagcgt gataccggtg
    15721 tcggttgacg gagtgattaa ccccatgctt cccgacagcc ggacggacca gggccaggca
    15781 gagcagcttg gcgtgaagtt cttccccgcg atgatgctgg ttaacccgaa aagtggccag
    15841 gtgaagcctc tcagttacgg ctttatcagt caggacgacc tggcgaaaca gttcctctat
    15901 gtctccagcg actttaaacc gaatttctaa ggagtgtgtc atgcgaaata ttcgttttcc
    15961 ggatttagat atcaccggga tgtgggtgct ggccgtgggc gtgtttttcc acctgatagc
    16021 ccggcttgtc agaaaacagc ctgagctggc cgtccaggcc ggggaaatct tcgggctggg
    16081 tatggtggtg ttcgggggat accgcattct aaacgccctg attgctgaag ccgaaaaaga
    16141 ggagaaagcc tgtgaaaaac agcctgatga acctcgctaa gacgccgctg gcggcactgg
    16201 ctctttccct tgcactggcc ggggtggcac atgcgggaac gctcgatgag gtgaagtcgc
    16261 tctgggatcc gcggggtatc tccggtactg aaacggcctc cccgcagagc gacactcccc
    16321 aggcggtgag cggggcatct cagccacgct ggttacgact cagcaacgga aagcaggtgg
    16381 acctgcgcga ctggaaagtg gtgctgttca tgcagggaca ctgtccctac tgtcataagt
    16441 ttgacccggt gctgaagcag ctggccggac agtatggctt ctccgtgttc tcttacacca
    16501 ttgacggaca gggtgatgac gcgtttccgg aagcgctgcc ggcgcctcct gacgtgatgc
    16561 agaccttttt ccccaatatc ccggtggcca cgccgactac gtttctggtg aacgtaaaca
    16621 cgctggcagc gtacccgatc ctgcagggcg ccacggatgc acagggcttt atggccagag
    16681 tggatacggt atttcagatg atgcactgag tctctgttat atccggtctt agcaagtgat
    16741 taaggaaaat agccatgaac aaatatatac gttcgacggg actgtatgcg tttttatttc
    16801 cagccagttt aaaggactcc gggcagacag cagcagaaag aatagaacaa ctgaagccgg
    16861 aatttatcca ccgggaaagg cgtctggaaa tctatctgga attattcatt gtgtttctca
    16921 ctgcaggtgc tctgctgtta tggataatga gatttttatt taatctctgc gtggatgact
    16981 ggatagccag cggtgacctg cgagtaaaag acctgtggaa tattatgatg tacgccatcc
    17041 cttatgcgct gatcgctgtt ggggtggggt tctttgttgc cggagtgaca ttagcaatcc
    17101 gcaatttttt cagttatcac cttaagaccc tttttattct gagaaatgac agagttaaaa
    17161 agaatgctgt gcgtaatgga ggacaggatg cgaattaaac ttctgtcact tttctgcggt
    17221 gccgcgcttg gcctgtcatt tagcgccgcc gccgacgtga atggcgacat gaataacttc
    17281 ttcaataagc tcgggtttgc gtccaatacc agccagccac aggtctggca ggggcaggcg
    17341 gcgggttatg cctctggcgg ttcgctgtat gccagaacgc aggtgaaaac gatccagctg
    17401 gtcagcatga ccctgccgga tattaacgcc ggctgcggcg gcatcgatgc gtacctgggg
    17461 tcgttctcct ttatcaacag cgagcagctg cagcgctttg gcaaacaaat tatgtccaac
    17521 gccgcgggtt acttctttga ccttgccctg cagaccacgg ttccggagat caaaaccgca
    17581 aaggacttcc ttcaaaagat ggccactgac atgaacagca tgaacctcag ttcctgccag
    17641 gctgctcagg gaattgtggg tggcctgttt cctcgtaccc aggtcgctca acaaaaggtc
    17701 tgccaggaca ttgccggtga aagcaatatc ttttcggact gggccgcctc gcgccagggt
    17761 tgtacggtgg gcggcaagat ggacagcgtc caggataagg ccagcgataa agacaaagaa
    17821 cgtgtgatga agaacattaa catcatgtgg aatgccttat cgaagaaccg gctctttgat
    17881 ggcaataagg agctgaaaga gtttgtgatg accctgaccg gtacgctgat ttttggtgag
    17941 aacagtgaga tcaccccgtt gccggcacgc accactgacc aggatctgat taaagccatg
    18001 atggagggcg gcacagctaa aatctaccac tgcaatgact cggataaatg tctcaaggtc
    18061 gttgctgatg cgaccgtaac catcaccgcc aataaggcgc tcaaaaatca gattcgcgct
    18121 ttgttgagca gtatccagaa taaagcggtg gctgatgaaa aactgacaga gcaggagaaa
    18181 gggtttatct ccagcaccac cattccggtg ttcaaatacc tggtcgatcc gcagatgctc
    18241 ggggtgtcta actcacttat ttaccagctg acggactata tcggctacga catcctgctg
    18301 cagtacattc aggaacttat ccagcaggcc cgggcgatga tctcaaccgg taattatccc
    18361 cagtcgacaa tggatatgat catggaaaat ctcaaccagg cgagtgtgca gattgccgct
    18421 ttccagtccc gtgttcaggt ccagcaggac gccttgcttg tggttgacag acaaatgagt
    18481 tacatgcgcc agcaggtctc tgcccgcatg atgacccgct accagaataa ctatcatttt
    18541 ggggggaatc tgtaatgctt gagatatatg ccattgctgg cggcgactgg ttacggggca
    18601 acctgaacgc gattgcggcg tttatgggga ccagcacatg gtcgactata gaaaagatgt
    18661 gtatcgccat ctcggtactc attgtggcgg ggaactgggt aaaaaaacac aacgtaatgg
    18721 accttattgg ctgggtcttt tccctcaccc tggtctctat gctggttgtc attcgtaccc
    18781 ctgtgcagat catcgattac agtaacgttg cgcaggtcta tgaagtcgat aatgttccta
    18841 ttggtcttgc cattcctgcg tcgctgacaa cgagggtggg aaacgcgctt atccagagtt
    18901 atgagatggt cttcgcgctg ccggacagcg tcacgtacag taaaacgggg atgttatttg
    18961 gcagtaatct ggtcgcgaaa agtacggatt ttttgtcaca aaatcctcag attacgacgc
    19021 tgttttccga ttacgtacaa aactgtgtga tgggcgatat tttcctcaat cataagtact
    19081 cttttgaaga attactgaat tcacctgatc cgtataccct gatttttgca aacccaagcc
    19141 cactgcgtgg cgtgtttgat aaaaacaacc agttccagac atgcgaagag gcctcacggg
    19201 atctgaaatc ggctcttgcg cttgatactc agacaggcgg gaaaacctgg aactactatg
    19261 ttcgtcagct gtttggcggc aaacctaatc ccgatctgct tttcagccag atgattggcg
    19321 acagctacaa ttacttctac agctcgggac agagcgccgg gcagattatc cgccagaacg
    19381 tcacgatgaa cgccctgcgc agtgggatcc agagttatgc ggcccgcagc ggggacaccg
    19441 ccagtctggt caatatggct aacacatcat cccttgaaaa gcagcggctg gcacaggcca
    19501 caatggggca ccaggcgctt cgcgcacttc cactgatgca gaccgtcatt atgggtttaa
    19561 tgattgggat gttcccgatc atggtgatgg ctgccatgtt taacatgatg acgctgcagg
    19621 tgcttaaagg gtatgtgttt gcgcttatct ggctgcagac gtggccatta ctgttcgcca
    19681 tcctgaacag cgcgatggcc tactatgcga agcaaaatgg cgtgccggtt gttctctctg
    19741 agttgtcgca ggttcagctc aaaaactcgg atattgcgac caccgcgggt tatattgccg
    19801 tgatgatacc gcccttgtcg tgggggatcg tgaagagcat gggggccgct ttctccagtg
    19861 cctacagcca tttttcttct tcggggctca gtgcaacatc gcaggcggcc tccggcgtgg
    19921 tggacgggaa ttactctttc gccaacatgc agatggagaa cgtcagcggc tacagctggg
    19981 gtaccaacag tacaaccagt ttcggacaga tgtcgcggca gctggcgaat gggggtatga
    20041 gcacgcagac gcgggatgga tcgatggtct gggacagtgg tggtgcaatg tcaaaactgc
    20101 cggtggatat caacgtcggt cgtcagatag ccagtgcgca gcaacagatg gcccgtgagg
    20161 ctgatgtcca ggccgaaagc gcgctgcatg gctataacag cagcgtgaca agtgcctgga
    20221 atagtctgca gcaatttggc accaataaag ggaacagcac atcaacaacg acaggggccg
    20281 atactaccga gtcttcgcag gattcgatgg ccagaagcaa gatgtggaat gcggttgttg
    20341 ctaatgcgaa agccaacaac atcagtaatg aggagtcttt ccagcagttg atggatgatt
    20401 catcgaaaag aactcaatca tttgatgcac atgtatctgg taaatggagt tctggggatc
    20461 agctgtttgg caagttagga aagtggggca gtggtttttc tgctgaagtt ggtggtaaag
    20521 gaagtgcagg ctggggacac agctcaggta atacagataa cgttggaact tccggtaggg
    20581 aatcgaatga cagccgacat gataccagca gtcaggcggc caaagacttt aaggaagcat
    20641 ccgattactt taccagccgc aaaaccacca catcgggcaa tattaccgat aataatgcca
    20701 gctcgcgtgt tgaccagttt gccgcgtcac tctccagtgc caaaaacagc tatgaccagt
    20761 atacaagcag ccggacacgc agccatgagt acagtgagat ggcatcacgc acagagagca
    20821 tgactggcca gatgaacgaa aacctgactc agcagtttgc gaactttgtt cagcatcgtg
    20881 ctccacagga tgccgaagcc attctgacca ataccagttc tcctgaaatt gcggcgcagc
    20941 gtgaagccct ggcgcgtgaa ttcgtcaaag agcaggtaga acccagggtt gatgctgcgt
    21001 atcaacaggg gcgtgaaagc atcggacaga atatggctgg ggtgtcaggt ggtggtgaca
    21061 atggttcggt aatggccgac tacggacaaa attctcgtcg tatcgatgtt atgacacaag
    21121 atgcgggtat taaggataac gtgggtcaga aggtggatag gatgattaca gagaataaac
    21181 aacagcatca ggaaacccgc gaaaacatac aacggcaagg cgcagaggtg aaaagtgaaa
    21241 atgctgaaat ggaaaagaac cataaaacta aagcaaatga gtttaaaggt gattataatg
    21301 aaagaaagag taaggtaaat tctttacctg gagctgactc tcaggctgag ttagaggaaa
    21361 aagcggcgaa aacgcaaaag gattttcttg atgggaaaag atgaaaatga tgatgaccaa
    21421 taaaatcctc attgaagaag tagatgagct gaaaaatgtg ttttcacgag gtgaaatgga
    21481 gataccatct ttttggtcat gtctatggcc tggagtaatt gttgccgcct ggaatatgat
    21541 ttgcgcccta attagtgtag ggggcgggta tttgtctgtt aagttcatat tttgggtgtt
    21601 ggtgttcccc ggtgttataa gtcttattgt tctgcttggt gtggcaagtg cgcgttcact
    21661 ttttttatct gttcccagaa cctatagaat gcgctcgcag gtatatcggt ttatatctag
    21721 aaaagttatg gtctatgcac ttgtgtatat ggctttaatt ctagcgcttg cactatttaa
    21781 tagagtgtat tatgcctctc catttccttt ttcgttcatg attcttattg cgacgatttt
    21841 tgtatggttt gttatgaata ttgattttgg acgatatcaa ctttcggtac tctcatctgc
    21901 gataaactct ttcagagcag aaaagttggg ggggggatga tccagaggca aactatcaat
    21961 tatggtggtt tgttaacagc ctaaaagttt cctttgttat tattggtttc ttagactgta
    22021 attagttttc acatctcgtt taaaattcgt atatagtgaa tgcgcattcc gaaatcataa
    22081 gagaatgtgt attcactcgt attgattatg tcacaaggat tcaggatgaa gcttaataaa
    22141 ttcatggctg tgatggtcgt cagctctgct ctggtgttgt ctggatgtag tgcaatggga
    22201 accgctatta aaaagagaaa tctggaagta aaaacccaga tgagcgaaac aatctggctg
    22261 gaaccttcaa ataataagac ggtttatctg caaatcaaaa atacctccga taaggatatg
    22321 agtggcctgc aggcaaaaat tgccagcgct gtcacttcaa agggatatca ggtcgtcagt
    22381 aaccccgata cggcgggcta ctggatccag gcgaatgtgc tcaaggccga taaaatggac
    22441 cttcgtgagt ctcagggctg gttaagccgg ggttatgagg gcgctgtgac tggcgccgct
    22501 cttggggcgg ggattaccgc ttacaactcc agttccgcgg gagcaaccct gggagtaggg
    22561 cttgcagccg gtctggtcgg aatggcggct gacgcgatgg tggaagatgt gaactacacc
    22621 atgatcaccg atgttcagat cgcggaaaga accaaaacac aggttcagac tgataacgtg
    22681 gccgtattac gtcagggaac atccggagca aaagtgcaga caagtactga aacgggtaat
    22741 cagcataaat accagactcg cgtcgtctct aacgcgaata aagttaacct caagttccct
    22801 gaagcccagc ctgttcttga agaccagctg gcaaaatcga tcgctaatat cctgtaaacg
    22861 ttctattcca cggcccgggt actccgggcc agactggcat cacctttccg gcgtgtcaga
    22921 attcattcgt caaataatca gttaattcca catctaaacg gtgaacagtt gcgcaattta
    22981 tttgtgcatc tgtgaggcgt tgccgccttc ggcggagtct tttcgtcttt atttgaacag
    23041 aggttattta tgacaatatc tgtgcctgtc aggtctcttg atacacatga actgatgcct
    23101 ggcgatattt acccacatcc tgcagcgggg ggaattgatt ccctgtcaca tcgcgaggtc
    23161 tgttctctct tcagtgacca catcatgtat atctgcagcg cgttagttat tcttctgctt
    23221 gttttcatcg caggtgtctt tttccgaaaa ataatgacgt cttccgtgga gcgtaagcga
    23281 aacacggcgt cagttatttc cgatgcaaag ccagaagtac agagcaaacc ggaatacttc
    23341 catctttcca tatgtgtgga aggcccggcc agcacggatg actccgcctt gtcctctctt
    23401 gcctcccgtg ttgtccgcga agcagaaaat ctgtatatcc ccggcgtcaa tcgccgcgac
    23461 agcgctgacg acgtgtttgc ctggtccgtg cagactcagt ttccttctga caggctcttc
    23521 ggcacacgga ttttcccgtc aaccggctgg tttgaacgtc cggatatgat taaacatccg
    23581 gtgaaatgct actgcgtctg cctgcagtac acgactgaaa cattgccatc cacactgtat
    23641 gccctggtcg atgaggccgg acggcaggtg gcgaagtcgt atattaaagg cgggcactgc
    23701 cctggctcag agggctatac cttctgcata acctcttaat agaagtcagt atccggcctg
    23761 gccatcaatg gatccgggca tgactgtaat cctgttttgt gccggtgtat ttccgctgat
    23821 taatttgtcc ttccgaccct aatactttac tgacggatta gtgccatgag tttcaatgcc
    23881 aaagatatga cgcagggcgg tcagatcgcc aatatgcgct ttcgcatgtt tggtcagatt
    23941 gccaatatta ttttctacgt gctgtttatt ttattctggg tgctgtgcgg gctgatgctg
    24001 atgtatcggc tcagctggca aacctttgtg aacggctgcg tatactggtg gtgtacgacg
    24061 ctgggtccga tgcgcgatat tattcgttcc cagccggtat acaccattca gtattacggc
    24121 cagtcgctgg aatatacctc agaacaaatc ctggccgata aatatactat ctggtgcgga
    24181 gagcagctgt ggaccagttt cgtctttgca gccgtggttt ctcttgttat ctgtatcgtg
    24241 acgttcttca tcgccagttg ggtgctgggc cgccagggga aacagcagag tgaggatgag
    24301 aataccggcg ggcgccagct ttccgataag cccaaagaag tggcccgcca gatgaagcgt
    24361 gatggtatgg cctccgatat taaaattggc gatctgccta ttcttaaaaa ctccgaaatc
    24421 cagaacttct gcctccacgg taccgtgggt tccggtaaat cagaagtaat ccgccggcta
    24481 ctgaattatg ttcgtgcccg gggcgatatg gccatcattt acgatcgctc ctgtgagttc
    24541 gtcaaaagct attacgaccc gtcactggat aaaatcctca acccgctgga ctcgcgctgc
    24601 gcggcctggg atttgtggaa agagtgcctg acgctgccgg acttcgacaa catctccaac
    24661 actcttatcc cgatgggcac caaagaagac ccgttctggc agggctccgg tcgcactatc
    24721 tttgccgaag gcgcgtacct gatgcgtgaa gacaaggatc gcagctacga gaagctggtt
    24781 gataccatgc tgtccattaa aatcgacaag ctgcgcgcct atctgcagaa cacgccggca
    24841 gcgaacctgg ttgaagagaa aattgagaaa acggccatct ccattcgtgc cgtgctgacc
    24901 aactacgtca aggctattcg ctacctgcag ggcatcgaga agaacgggga gccgttcact
    24961 atcagggact ggatgcgcgg tgtccgtgag gaccgaccca atggctggct gtttatctcc
    25021 tccaacgctg acacccatgc ttccctgaag ccggttatct cgatgtggct gtcaattgct
    25081 atccgcggcc tgctggcgat gggggagaac cgtaaccggc gcgtgtggat tttcgccgac
    25141 gagctcccca cgctgcacaa gctgccggac ctggtcgaaa tcctcccgga ggcgcgtaaa
    25201 tttggtggtt gttatgtgtt cggtattcag tcctatgccc agctggaaga tatctatggc
    25261 gtcaagcctg ccgccacgct ctttgacgtg atgaacaccc gagccttctt ccgctccccg
    25321 agtaaagaga ttgcggagtt tgctgccggt gaaattggcg agaaggaaat cctcaaggcc
    25381 agcgagcagt actcctacgg tgctgacccg gtgcgtgatg gtgtatcgac cgggaaagaa
    25441 aaagagcggg aaacgctggt cagttactcc gatattcaga cgctgcctga cctgtcctgc
    25501 tatgtcacgc tgcccgggcc gtatccggcc gtgaaactgg ccctcaaata caaaccgcgt
    25561 ccgaaaattg ccgagggatt tattcctcgc actctcgatg cgcgggtgga tgcgcggctc
    25621 agtgcgttgc ttgaagcgcg ggaagctgaa ggcagtcttg ccagggcact tttcacgccg
    25681 gatgcgccgg catccgggcc cgctgataca aacagtcacg ccggtgaaca acctgagccg
    25741 gtcagtcagc ctgcgccagc agacatgaca gtatctccgg caccggtaaa agcgccgccg
    25801 acaacgaaga tgccggcaga ggaaccttcc gtcagagcaa cggagccttc cgtcctgcgg
    25861 ctcacgaccg taccgttgat caagccaaaa gcagccgctg ctgccgctgc agccgcaaca
    25921 gcatcctctg ccggtacccc tgctgcagct gcaggcggga cagaacagga gctggcgcag
    25981 cagtctgcgg aacagggaca ggacatgctg ccggccggca tgaacgagga cggtgtcatc
    26041 gaggacatgc aggcgtatga cgcctggctg gcagatgagc agacacagcg ggatatgcag
    26101 cggcgtgagg aagtcaatat caaccacagc caccgccacg atgaacagga ttatgtcgag
    26161 attgggggga acttctgatg atgtcgattg gctccgttaa atcggcgggc agtgccggta
    26221 attactatac ggataaggat aattactacg tcatagggag catggacgaa cgctggcagg
    26281 gtaagggggc tgaggcgctg gggatcgacg ggaaagccgt ggataaggcg ctgtttacgg
    26341 aactgctgaa agggaagctg ccggatggca gcgacctgac ccggatacag gacggcgcca
    26401 acaagcaccg gcccggttat gacctgacct tctcggcccc caaaagcgtg tcggtcatgg
    26461 cgatgctggg cggcgataaa cgccttatcg atgcccacaa ccaggcggta accgaagcgg
    26521 tgcggcaact ggagacgctg gccgcgaccc gggtcatgac ggacggtaaa agcgaaacgg
    26581 tgctgaccgg aaatctgatt gtggcgaaat tcaaccacga cacgaaccgt aaccaggaac
    26641 ctcagataca cacccatgcc gtcgtcatta acgccacgca gaacggggac aaatggcagt
    26701 ctctcggcac agataagatc ggtaaaacag gttttatcga gaacgtgtac gcgaaccaga
    26761 tagcgttcgg gaagctttac cgggaggcgt tcaaacccct ggttgagaag cttggctatg
    26821 agacggaagt tgtgggcaaa cacgggatgt gggagatgaa aggggtccct gtggaaccgt
    26881 tctccacccg gtctcaggag gtcagggagg ccgcagggcc cgatgcctcc ctgaagtccc
    26941 gggatgtggc cgcgctcgat acccgcaagt cgaaagacgc catcgatccg gcagagaaga
    27001 tggtggagtg gatgaacacg ctgaaggaga ccgggtttga catccggggg taccgtgagg
    27061 ccgccgatgc gcgagccgca gagctggccc gtgcacctgc cgcgccggtg aacactgacg
    27121 gtccggatat cacggatgtg gtgacgaaag cgattgccgg tctcagcgac cgcaaggtgc
    27181 agttcacgta tgccgacctg ctggcacgca cggtcggcca gctggaggcg aaggacggtg
    27241 tgtttgagct ggcgagaaag ggcatcgatg ccgccattga acgggagcag cttatccctc
    27301 tcgatcgcga gaaaggcctg ttcacctcaa acatccacgt tctcgatgaa ctcgccgtca
    27361 aagcccttag tcaggaggtg cagcgccaga atcatgtctc cgtcacgcct gatgcgtttg
    27421 tggtgcgcca ggtccctttc agcgatgccg tcagtgtgct ggcgcaggat cgcccggtaa
    27481 tgggtatcgt ttccggtcag ggcggtgcca ccggccagcg ggagcgggtg gccgagttaa
    27541 ccctgatggc ccgggagcag gggcgtgacg tccatatcct ggcggccgat aaccgctcac
    27601 gtgattttct cgccggtgat gtgcgtctgg ccggggaaac ggtgaccggt aaatccgccc
    27661 tgcaggacgg gacggcgttt attccgggcg gaacactcat cgtggatcag gcggagaagc
    27721 tgagcctgaa ggaaacgatt tccctgctcg atggtgcgat gcgccataac gtgcaggtcc
    27781 tgctctccga cagcgggaag cgcagcggga ccggcagcgc gctgacggtg ctgaaggaca
    27841 gcggcgtgaa tacgtatcgc tggcagggtg gccatcagac cacagccgac atcatcagtg
    27901 aaccggataa aggagcacgc tacagccggc tggcacagga gtttgccgtc agcgtgcgtg
    27961 aagggcagga aagcgtggcc caaatcagcg ggacgcgtga acagagcgta ctcaacggcc
    28021 ttatccgtga ctccctcaga caggaggggg tgctgggtga gaaagacacg accattacag
    28081 ccctgacgcc ggtgtggctg gacagcaaaa gccggggcgt gcgggattat taccgggagg
    28141 ggatggtgat ggaacgctgg gatccggaga cccggacgca cgatcgcttt gtcattgacc
    28201 gggtgaccgg gagcagcaat atgctgaccc tgaaagaccg ggaaggggtc cgtctggatc
    28261 tgaaggtttc cgcggtcgac agccagtgga cgctgttccg ggcagataca ctgccggtgg
    28321 cagaggggga acgtctggcg gtgctcggga agataccgga cacacgcctg aagggcgggg
    28381 aaagcatcac ggtgatgaaa gtggaggacg ggcagctgac ggtacagcgg ccggggcaga
    28441 aaaccacgca gaccctggcc atgggcgcgg gcgtgtttga cggtattaaa atcggccacg
    28501 gctgggtgga aagtcccggc aggtcggtta gcgagacggc aacggtcttt gcgtcggtga
    28561 cgcagcgtga gctggataac gccaccctca accagctggc gcagagcgga agtcatctcc
    28621 ggctgtactc ggcgcaggat gcggcgcgca cgacggagaa gctgtcgcgg cataccgcct
    28681 tcagcgtggt ctcagagcag ctgaagaccc gatccggcga gacggacctg gatgcggcca
    28741 ttgcgcagca gaaggccgga ctgcgtaccc cggcagaaca ggccattcac ctggccatcc
    28801 cgctgcttga aagtgagaaa ctgaccttct cccggcccca gctgctggcg acggcgctgg
    28861 aaaccggggg cgggaaggtt tcgatggcgg acatcgacac gaccatccag gcacaaatca
    28921 ggtcggggca gttgctgaat gtaccggtgg cgcacggcta cggcaatgac ctgctgattt
    28981 cccgtcagac ctgggacgcc gaaaaaagca tcctgaccca cgtgctggaa ggtaaagacg
    29041 cggtggcccc cctgatggac cgcgtgccgg ccagtctgat gacggacctg accgccggac
    29101 agcgggcggc gacccggatg attctggaaa gcacggaccg gttcacggtg gttcaggggt
    29161 atgccggggt ggggaaaacc acccagctcc gggcagtcat gagtgccatc agtctgctgc
    29221 cggaagagac ccgtccgcgc gtcatcggtc tggcgccgac gcatcgcgcg gtcggggaaa
    29281 tgcagagtgc tggcgtggat gcgcgcacga cggcatcgtt cctccacgac acgcagctgc
    29341 tacagcgcaa tggccagaca cccgatttca gcaacaccct gttcctgctc gacgagagct
    29401 caatggtggg gctggccgat atggcaaaag cccactccct catcgtcgcc ggcggtgggc
    29461 gggcggtgtc cagtggggat aacgaccagc tgcagcccat cgcgccgggc cagccgttca
    29521 ggctgacgca gcagcgcagc gccgccgacg ttgccatcat gaaagagatt gtacgccaga
    29581 tgcctgagct gcgaccggcg gtctacagcc tgattgagcg ggatgtgcac cgcgccctga
    29641 ccaccatcga gcaggtcacg ccggagcagg tgccgagaaa agaaggcgcc tgggcgccgg
    29701 gcagttctgt ggtggaattc acgccaaagc aggaaaaggc gatagaaaag gcgctgagcg
    29761 aggggaaaac cctgccggaa ggtcagcccg cgacgctcta cgaggcgctg gtgaaggact
    29821 ataccggacg gaccccggag gcacagtcgc agacgctggt gatcacccat ctgaataagg
    29881 accggcgggc gctgaacagt ctgatccatg acgcacggcg ggagaacggg gaaaccggca
    29941 aagaggaaat caccctgccg gtgctggtca cgagtaacat ccgtgacggt gaactgcgca
    30001 aactgtcgac ctggacggct cacaaggagg cggtggcgct ggttgataac gtataccacc
    30061 gtatcagcaa ggtggataag gctaatcagc tgatcaccct gacagacagt gaaggaaagg
    30121 agcgatatat ctcaccccgg gaggcatcgg cagaaggcgt cacgctctat cgtcaggaaa
    30181 agataaccgt gtctcagggg gaccgaatgc gcttcagcaa aagtgacctg gagcgcggct
    30241 atgtggccaa cagtatctgg gaggtgcagt ctgtttcggg tgacagtgtc acgctatctg
    30301 atggcaaact cacccgtacg ctgaccccga aggcggatca ggcgcagcag catatcgacc
    30361 tcgcctacgc cattacggcc cacggcgccc agggggccag cgaaccgtat gccatcgcgc
    30421 tggaaggggt ggccggtggt cgggaacaga tggccagctt tgagtctgcc tatgtggcgt
    30481 tatcccgcat gaaacagcat gttcaggtct acaccgacaa ccgtgagggc tggataaagg
    30541 ccatcaagca ttcacctgag aaagcgacgg cgcacgatat tcttgaaccc cgtaacgacc
    30601 gggcggtgaa aacagccgat ctgctgttcg ggcgggcaag gccactggat gaaaccgccg
    30661 cgggccgggc agccctgcag cagtccggtc tggcacaggg aagttcacca ggaaaattca
    30721 tttctccggg gaaaaagtac cctcagccgc atgtggcgct gccggcattt gataaaaacg
    30781 ggaaagccgc cggtatctgg ctcagcccgc tgaccgatcg ggacggacgg ctggaggcta
    30841 tcggcggaga gggacgaatt atgggcaatg aagacgcccg gttcgtggcg ctgcagaaca
    30901 gccgcaacgg agaaagcctg ctggccggga atatggggga gggcgtcaga atggcccgcg
    30961 acaatcccga tacgggggtg gtggtgcggc tggccggtga tgaccgcccc tggaaccccg
    31021 gggccatgac cggtgggcgg gtatgggctg aaccggcgcc ggtggctccg gtaccgcagg
    31081 ccggggctga cattatcctc ccgccagagg tactggcaca gcgggcggca gaagaacagc
    31141 agcgccggga aatggaaaaa caggcggaac agactgcccg ggaagtggcc ggggaggcgc
    31201 gtaaagccgg cgagccggcg gaccgggtga aagaggttat cggggacgtc attcgcggac
    31261 tggagcgtga ccggccgggt acggagaaaa cgacgctacc ggatgatcct cagttccggc
    31321 gccaggagga ggcgattcag caggtggcct cagaacgtct gcagcgtgag cgtctgcagg
    31381 ctgtggagcg ggacatggtt cgtgacctta accgcgagaa aacactcggg ggcgactgac
    31441 gcgcctgcgg cttgtctgct ccggcagcct ccccgattga aatcgggcag tctgccggag
    31501 cgctaaaagg aaaaaaaaca tgagagcaca acacacattt ccaggcgcga cagacagctg
    31561 gcttcagcgc ctgctgacct ggacaccggg acaaacggat atgattaaaa cggtcgcact
    31621 ggtactgatg gtgactgacc acaccggctt actgctggcc ggaaacaatg aagtgatgcg
    31681 cctgctggga cgtggttgtt ttccgctgtt cggtctggca tggggaatga atctggcacg
    31741 gcacgcggaa atacgccagt ctcaactgaa cagcctgtgg ggctgggcgc tggtcgccca
    31801 ggtgtcgttt atgctcatcg ggtacccgtg gtataccggg aatattctgt ttgcctttgc
    31861 cgttacgggc caggtcctgc ggtgggtcag ccagccgtcc tggtattaca ccctcccggc
    31921 ggctggactg ctggtcacct ggatacccct gtcaaccggc agctacggca tggccggagt
    31981 gggcatgctc accgccagct ggctgctttg tcgcgcacag catgcaccgg aacggctggg
    32041 atgcgtattt tcatgaaagg agatcactca ataacttcca tcgagatcgg gtaataacat
    32101 ttgaacagat cgctgaataa catcgatgga gatcactttt gactcatttt gttattcagt
    32161 gatctccatc aatgttattg gaacttcaca ggtgtgttga tctgtatctt ttgccattcc
    32221 ggtaaaggat acctatgcca acagttccaa tttctatgag aaaacttaaa gaaattctta
    32281 ggcttaaata cggtgttgga ctcagccatc gacaaattgg tcgtagtctt gcaatctccc
    32341 cttccgttgt atccagatat gctaatcggg cggctcaact tggcataaag cagtggccct
    32401 tacctacagg atgggatgat acaaaactaa aacatgcgtt ccttcagacc caggttaaga
    32461 tgaagaagca ctctctgcct gactgggcta cagtacaccg ggaactgcgt aataaatgcg
    32521 tgacgctgca gctactctgg gaagaatact gtgagcgtaa tccaggcggt ttttacagct
    32581 ataaccatta ctgccggatg taccgtgaat ggctcaaaac cacttcacca tcaatgcgtc
    32641 aggtacataa agctggcgaa aaacttttcg ttgattactg tggacctacc gttggcgtta
    32701 ccgaccctga gaccggagaa ataagaactg ctcaggtcat cgtagctgtt ctcggggcat
    32761 caagttacac atgggcagag gccacctggt ctcagcagct tgaagactgg gtgatgagtc
    32821 atgttcgctg cttccagtgg ttgggtggcg ttcctgaact tgttgttccg gacaatctga
    32881 aaagcgccac atccagggca tgtaagtatg atcctgacgt taaccctacc taccagcaga
    32941 tgcttgagca ttataatgtc gcagttttgc ctgcgcggcc acgtaaaccg aaagataaag
    33001 ccaaagctga agttggcgtt caggttgttg aacgctggat catggcccga atcaggcatg
    33061 agatcttcta cagccttgca tcgcttaatc agcgcattcg ggagttgctg gaaagactga
    33121 ataacaaaat aatgcagaag ttgggttatt cacgtgcaga actcttcatc cagcttgata
    33181 aacccgcact gaagcctctt cctgaagcca gttacagtta caccctggtg aagaaagtca
    33241 gagttcatgc cgattaccac gtggaaatcg acaaacatta ctactcggtt ccatgttcgc
    33301 tgttaggcca gcaactggaa gcatggatct ccggagaact ggtaagactc ttcaatcagg
    33361 ggcaggaggt tgctgtgcac ccgcgcaagc gtacttatgg ctacagtacc cgcaacgagc
    33421 acatgcctga agctcatcga cagcatgcca cctggacgcc agagcgtctt ctggaatggg
    33481 cggggcacat aggcagtgaa actcatagtt atgtgcttca tatactgaac tctcgtccac
    33541 atccggaaca aagctatcgc ttctgccttg gactcctgaa ccttcataaa aaatacagta
    33601 aagccagact taatgcagca tgtgcaagag ctctgaaaac aaaggtatgg cgtctgtcag
    33661 gtattaaatc gatcctggaa aaaggtctgg ataaacaacc tgttcaggat ccaaaaccag
    33721 atctgttatc cacgatggaa cacgaaaacg tacgcggcag tgagtattac cactgatacg
    33781 ggatccaatg atgaatcatc tttacgaaca actgaccgca cttaaactca ccggcttccg
    33841 tgatgcgctt aaaaagcaac ttgctcaacc gggcacatac caggagctgg gcttcgaaga
    33901 acgcctgtca ttactgacag cagaagaact aacctgccgt gaaaacagga aggcagagcg
    33961 tctgatcaaa catgcacggt tcagacttaa tgctgagtta tcaaagctgg attatcgtaa
    34021 caatagaggg ctggacaggg ccctcatccg ttcactcagt cagggaaact ggttaaccct
    34081 gaaacaaaat attttactga ccggggccac cggcagcggt aaaacgttcc tggcatgtgc
    34141 acttggtcat aatgcctgcc gacagggata caaggtctac tattatcgcc ttaaagcgct
//
LOCUS       DACADF010000038        33918 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.038, whole genome shotgun sequence.
ACCESSION   DACADF010000038 DACADF010000000
VERSION     DACADF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.038"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(100..1005)
                     /locus_tag="ISB30_001729"
     CDS             complement(100..1005)
                     /locus_tag="ISB30_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1989368.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNQQLMTRFVFN"
     gene            complement(1083..2306)
                     /locus_tag="ISB30_001730"
     CDS             complement(1083..2306)
                     /locus_tag="ISB30_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HAP1989369.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(2332..2721)
                     /locus_tag="ISB30_001731"
     CDS             complement(2332..2721)
                     /locus_tag="ISB30_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HAP1989370.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(2738..3694)
                     /gene="arcC"
                     /locus_tag="ISB30_001732"
     CDS             complement(2738..3694)
                     /gene="arcC"
                     /locus_tag="ISB30_001732"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP1989371.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAINFGTPQQQWLDAISVAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(3687..5111)
                     /locus_tag="ISB30_001733"
     CDS             complement(3687..5111)
                     /locus_tag="ISB30_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAP1989372.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMARYLEAQPVLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELTPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRISGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVAKENHHG"
     gene            complement(5108..6667)
                     /gene="fdrA"
                     /locus_tag="ISB30_001734"
     CDS             complement(5108..6667)
                     /gene="fdrA"
                     /locus_tag="ISB30_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HAP1989373.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALILAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIVQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSKEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNAQPLPAVETDVPCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNA"
     gene            complement(6762..7622)
                     /locus_tag="ISB30_001735"
     CDS             complement(6762..7622)
                     /locus_tag="ISB30_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAP1989374.1"
                     /translation="MVQVTLCASSLGYLFPQDETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGGMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(7628..8296)
                     /locus_tag="ISB30_001736"
     CDS             complement(7628..8296)
                     /locus_tag="ISB30_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP1989375.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSRVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(8553..9362)
                     /locus_tag="ISB30_001737"
     CDS             complement(8553..9362)
                     /locus_tag="ISB30_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAP1989376.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLGGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAEHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            9624..10388
                     /gene="udp"
                     /locus_tag="ISB30_001738"
     CDS             9624..10388
                     /gene="udp"
                     /locus_tag="ISB30_001738"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAP1989377.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(10423..11913)
                     /locus_tag="ISB30_001739"
     CDS             complement(10423..11913)
                     /locus_tag="ISB30_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HAP1989378.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(11926..12432)
                     /locus_tag="ISB30_001740"
     CDS             complement(11926..12432)
                     /locus_tag="ISB30_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HAP1989379.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(12449..13423)
                     /locus_tag="ISB30_001741"
     CDS             complement(12449..13423)
                     /locus_tag="ISB30_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HAP1989380.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASWPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPGGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRADAPWNSYEEFMTYAKANPGKVRIGN
                     SGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVKG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(13448..14077)
                     /locus_tag="ISB30_001742"
     CDS             complement(13448..14077)
                     /locus_tag="ISB30_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAP1989381.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFTAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYHPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(14067..15071)
                     /locus_tag="ISB30_001743"
     CDS             complement(14067..15071)
                     /locus_tag="ISB30_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAP1989382.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQPAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPSLQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDELHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEQGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(15095..15862)
                     /locus_tag="ISB30_001744"
     CDS             complement(15095..15862)
                     /locus_tag="ISB30_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP1989383.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            16079..17506
                     /gene="rmuC"
                     /locus_tag="ISB30_001745"
     CDS             16079..17506
                     /gene="rmuC"
                     /locus_tag="ISB30_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAP1989384.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYQTQVSIETDTRSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQNLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            17601..18356
                     /gene="ubiE"
                     /locus_tag="ISB30_001746"
     CDS             17601..18356
                     /gene="ubiE"
                     /locus_tag="ISB30_001746"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAP1989385.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18370..18975
                     /gene="ubiJ"
                     /locus_tag="ISB30_001747"
     CDS             18370..18975
                     /gene="ubiJ"
                     /locus_tag="ISB30_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAP1989386.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            18972..20612
                     /gene="ubiB"
                     /locus_tag="ISB30_001748"
     CDS             18972..20612
                     /gene="ubiB"
                     /locus_tag="ISB30_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAP1989387.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            20691..20960
                     /gene="tatA"
                     /locus_tag="ISB30_001749"
     CDS             20691..20960
                     /gene="tatA"
                     /locus_tag="ISB30_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAP1989388.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            20964..21479
                     /gene="tatB"
                     /locus_tag="ISB30_001750"
     CDS             20964..21479
                     /gene="tatB"
                     /locus_tag="ISB30_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAP1989389.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETVHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            21482..22258
                     /gene="tatC"
                     /locus_tag="ISB30_001751"
     CDS             21482..22258
                     /gene="tatC"
                     /locus_tag="ISB30_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAP1989390.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22300..23082
                     /gene="tatD"
                     /locus_tag="ISB30_001752"
     CDS             22300..23082
                     /gene="tatD"
                     /locus_tag="ISB30_001752"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAP1989391.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVACGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNAPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23079..23567)
                     /gene="rfaH"
                     /locus_tag="ISB30_001753"
     CDS             complement(23079..23567)
                     /gene="rfaH"
                     /locus_tag="ISB30_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAP1989392.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            23734..25227
                     /gene="ubiD"
                     /locus_tag="ISB30_001754"
     CDS             23734..25227
                     /gene="ubiD"
                     /locus_tag="ISB30_001754"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAP1989393.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25273..25974
                     /gene="fre"
                     /locus_tag="ISB30_001755"
     CDS             25273..25974
                     /gene="fre"
                     /locus_tag="ISB30_001755"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAP1989394.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26155..27318)
                     /gene="fadA"
                     /locus_tag="ISB30_001756"
     CDS             complement(26155..27318)
                     /gene="fadA"
                     /locus_tag="ISB30_001756"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAP1989395.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27328..29517)
                     /gene="fadB"
                     /locus_tag="ISB30_001757"
     CDS             complement(27328..29517)
                     /gene="fadB"
                     /locus_tag="ISB30_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAP1989396.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDVVVDDLLAKVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            29707..31038
                     /gene="pepQ"
                     /locus_tag="ISB30_001758"
     CDS             29707..31038
                     /gene="pepQ"
                     /locus_tag="ISB30_001758"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAP1989397.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31038..31652
                     /locus_tag="ISB30_001759"
     CDS             31038..31652
                     /locus_tag="ISB30_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAP1989398.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            31691..33142
                     /gene="trkH"
                     /locus_tag="ISB30_001760"
     CDS             31691..33142
                     /gene="trkH"
                     /locus_tag="ISB30_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAP1989399.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33154..33699
                     /gene="hemG"
                     /locus_tag="ISB30_001761"
     CDS             33154..33699
                     /gene="hemG"
                     /locus_tag="ISB30_001761"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAP1989400.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8056 a         8693 c         9016 g         8153 t
ORIGIN      
        1 aacagaccgg ttaattttca tgaattacgt caactgcaca aaaataattt aaacgggcgc
       61 tctaattaaa gcaagactta tatttttatc attgtattgt caattaaata caaatcgtgt
      121 cattaattgc tggtttctta aatattctat gcaccactga caggctttgc ccataccatc
      181 ggaacgagcg gcaagaaata acttggtggc gtgtttatgt tcgcgcattg gctttttaag
      241 caatttaccg ctgttcaata acggtaatgc cagatgatgt ggaatataac caataccgac
      301 gttttgttct attaaggcga ttgccgtagc gaagtcagga acaaaaatgg gtttttgtcc
      361 ttccaacaac caggcttgca tcggggggaa gttaacggag gtatcgcgga tacaaaccgc
      421 tggataatga cgcaattcac tgttttctaa tggatgccgc ttcgctgcca gtgggtggag
      481 tgggctgacg acaaaatccc attccatttg tccgaccggt tcgctaatga tgccttcact
      541 gcttggtact gcatgaggag caccgtagac cagatcagca cgcttactgt acagcgcatc
      601 ccagcagccg ttgtatacct caagatctac cgtcagtgtt gttgatgtga attgccgttc
      661 aaaatctcga ataaatgcca ccagcaaatc accaggaata atattgttta ccgcaattgt
      721 cagttcacgc tcaataccat cgtgaatcag cactgtgttg cgctttaatg cttccaggtc
      781 gccaagaata gttttgctat gttcgatgaa gtaacttccc gctggcgata attcaatata
      841 gcgtccctta cgaatgaaca gttcaacgcc caggctctct tccgctttct tgatggtgta
      901 actgatggcg gaagggactt tatttaactg ctcggctgcg gtggtaatgc tctggtgtct
      961 ggcgaccaga tgaataacac gaagcgtttc gtctgagatg aacataaggc tccctggtca
     1021 gtggtaaagt ggctgaatct ctccagattc agccgtaatc attgcctgag tatacccagg
     1081 aattagcggt gttgacttgc ccgactgaga atcgcatacg cgatacatgc caccagaatc
     1141 gagtctaccg aagggatcgt cgtcagggtg aataagcctt ttacggtcat aaagccaacc
     1201 gctgcgccta tgatccatgc tgcgaatgtc agcactttaa tctgtgactc ctgggctaac
     1261 actgactctt gatagccatt acggcgataa aggaaaaagt cagcgatata aattccggca
     1321 acgggtggcg tggcgatacc caggaacagc agaaacggaa taaaccacgc cataatatcc
     1381 atactgccta cgatggcgga aaggataccc agcgccacgg taatctgcca tttgggaaag
     1441 cgggtaagca gtgtggaaac caccagcgtg ccctggaaca tattgcccgc gttaccggtg
     1501 acgcaggcga aaatcaacaa cagcgccgca ggcagaactg caccgaatac cgccatcgca
     1561 cccagtaaag atccctgacc actaatggcg ctcggcgttg cacccgccca gtagagcaga
     1621 gggtaggcaa ccagaaacgt cacgcctgcg gcaatcagcg catgtttgcg gttatgcaca
     1681 aaactaccga aatcaggcag tgtggcaacc agcacaataa tggtgccaac aacggtggaa
     1741 acggcgacac ctgtattcat ctccacaatc gccgctggta ctgccacttc ggtatgggtt
     1801 gccacataga ggatgtaaca cagcaccagc gcgataaccg gcacggcaac ctgggcaatt
     1861 tttcccagaa cggcaaagcc aaatgccgta gaggcgacaa aaatgacaca gccagccgcc
     1921 actaacagtg ccagtggaac ttccagtcca tgttgagcca gtaagtcatg taccgaatga
     1981 ccaaacatat tggcggtaac ggcaatccag ccgaacagac ttatcgccat caaaatgtta
     2041 atggcgatgg cccctttttc gccaaacgaa tatttcacaa tgccatacgt tggcaggcgg
     2101 gctttttctc ccacgctaat ggtgatggct gaaagtaagg tcagaatgac gccgcctaac
     2161 aatacgacgc taattaaact gccgctggaa agttgattgc cgaggcttga ggatgacaac
     2221 agcacaggtg tgacggcaat acccagcaaa atcatggcaa tacgcaatcc tgatgccgta
     2281 ttcagattct cttctttttt acgcatagta tgttcccttt ataaatgcgc gttacacagt
     2341 atgaaaatgc ccggtgtatt tacgccagct acccagttct gtaatttccc gttggccttc
     2401 ggtcagccag ttttctgcca gtacgcccag cacttcgcta ccatcggcaa gtttgatttt
     2461 gccaattgcc agcccagcgg gttcgctcat taacagagcg gcaaaactgg ccagcggtaa
     2521 ttgccaaatt tctacgtcga cgtgtgttcc tccttcattc acgcgaatca ttcccggatg
     2581 gcgatcatta atactccaca accggtaatg ggcgtcagtt cggtcttcac ggacaaaaat
     2641 accgcccgct ttttgcatat ttgggttgag ttccaggccg cgcatcagcg ttccgttaac
     2701 ggctaaaagc gttgtagtca ttgtgttctc cagagaatta aagggttatc catgtaccgc
     2761 tctgatgcgc cagggctgct tttatggttt gcggtgaagt aatcaggccg ctggcctgtt
     2821 tgccttgttg ctggctggca ttgatgaaat caacaatggc ttccactttg ggttgcatac
     2881 tgccgacacc aaactgacct tcccgcaaca gcgtttgcgc ttcggcaacg ctgatagcgt
     2941 cgagccactg ttgttgtggt gtaccaaagt taatcgccac tttttctaca ccggtgggga
     3001 tcaccagcag atctgcgcct aactgactgg cgagcagcgc ggaggccaga tctttatcga
     3061 tcacggcttc tacacctttc agttgttgcc cgtctcgcac caccggaata ccgccgccgc
     3121 cgcaggcaat aaccagatat ccctggcgca ccaggtgagc gatggtgtcg tgctcaataa
     3181 tttccagtgg tgcaggagag ggaactgtac gccgccaacc acgcccggcg tcctccatca
     3241 gcgtccagcc gagttgttgt tggcgttgtt gggccgttgc ttcatcgaga aacgcgccaa
     3301 tcggtttact ggggctggcg aaagcatcgt catgtgggct gactcgcgtt tgtgtcacca
     3361 gggcaattac cggtttgttt atgccacggc gagcgagttc gttatgcagc gctttctgga
     3421 acatgtagcc aattgcacct tgtgtatcac ccacggcgta atcaagtgga accggagaaa
     3481 cttcgttact ggcgagttca gagcggcgca gaataaagcc cacctgcggg ccattaccgt
     3541 gggttagcac caggtcccat ccggcttcaa ccatatcaac gatatgctgc acgctttcca
     3601 tcactgcaac atattgatcg ggaatactat tgcgttgctc gtcctgaatg agcgcattgc
     3661 cgccaaccgc gacgacagcc agtggcttaa ccatgatggt tctcctttgc gacgatctcc
     3721 gccagtgcac gtacagcggc gacaaaacag gccatcggcg cagtggtgat cccggcacca
     3781 atttgcccga cccccgcttg tttgtgggcg atacccgtat tgatgaccgg taaaatgccg
     3841 cgatcggtca ctttacgcgc atcaatccct gctgcggtgg gggcaaaatt gagggctgga
     3901 agtgtaaatg cggggttacc gccgagggtg atggcttgca tccgacggct gttattggtg
     3961 gcatcggcag gggtgcctcc cacaaatttg acgatcgccg gagacgacgc catcgcgaag
     4021 ccacccacac ctgcggtttc agtaattgcg ctatcaccca ggtcagcagc agcgtcgtca
     4081 acgccaaatc cggggaagaa cagaccttca acggcattgg caggagcctg gaaccagcga
     4141 tcgccagtac cggaaatccg aataccaaaa ttcaccccgt tacgcgccat caccgtaacc
     4201 attgaactga acggtacatt ggcggcggca tccatcattg ctttacaggc ggccatcgac
     4261 agattgagga agaagtgatc gttaccggtg ataaatgcca cgacgcgctg gatatgttcc
     4321 tgtggaagag agcaggccag caatgccggg agcaggcgct taatcagcaa accggtagcg
     4381 gcagcattgc ggttatgaac ttcatccccc atatgcagcg cctgcgccat taatggcttg
     4441 agttccagct cgccgtgttg tgcgattgct gctttcattg ccggcgtcag ttcatcgcgc
     4501 atccaggcca gacgattgag aacttcatcg ttattggcgc caaaacggag aaccttaccc
     4561 agaccttcat tgaaattgct aaaagtacgg tggccgttgg ttttgttttc taccacccat
     4621 aacggcatcg acgggctgat gatgcctgcc attgggccaa ccgcgtgata atgatgacaa
     4681 ggggcaaggt cgatttcgcc tgcgtttatc tggttttcag cgtcttgctg gctggtggcc
     4741 caaccttcgt acagcatggc accaataatc gcccctttta ccggaccaca catctcttcc
     4801 caggcaatcg gcgggccaga gtgcaaaata cgtttttgtt cagccattgc tggaatggcc
     4861 tctttagcaa gcatgacatc caccagcacg ggctgtgctt cgagataacg tgccatggcg
     4921 atttggttgg cattttctac caggggatga cgtagcaacg atgccagatc caatccagcg
     4981 tcaatatcgc cttgtgctgg tggctgccag ttgagtgcaa ttgcgcttcc cccggcatgt
     5041 tgaatattgt cagcaaaact gtgtagtccc gcgttcacaa ccttcaaagg ctgattaaat
     5101 aaggtattca tgcgtttagc ccctttttct gcaactgagt ctgtgcaacg gtgctggccc
     5161 atagcgccgc ctgtgcattg ctgctggcga tgatgacccc tgcattttgt agtgcgctta
     5221 tctgctgact gcgtatttgc ggatcagctt ccgttccgca aacgtgcgca atcagcaacg
     5281 gagcgttatt catgtcgatg tgtgaaagtt ggtccagtaa ttcgctggca ggcgtcgtgg
     5341 aagcgccata accaagtacc aggtcgaata acacgacggc agtgtgtgag tcgttgagtt
     5401 cgttgagcag acgttgattg cgcagagtcg ggtcgatcat tggatgcggt ttgccacggg
     5461 tgaaatcatc gtcgcccata tcgatcaacg tgtgatcttc gctttgccag atatttgcca
     5521 gagcacgatt acccgcaact ggcgtattgg aagctgcgct gaatcccttt tgttggcaga
     5581 taagttgcgc ttcgtagcag aaggttcctc cggcgaaaac accgcgtatc gcctggcgat
     5641 gggctggcag gcgctggcag gcgttttgta acattgtgag atcgtcgcag ggtacatcgg
     5701 tttcgacagc cggaagtggc tgagcattca gtaacgccac agcgatgtcg gcggcgctgg
     5761 caagtgtggt ggcggcagtg atattgggac gtgccagatc atgcggattc gccccgagga
     5821 aattcaccac cacgggtttt ccacaagcct cggcgcgctc cagaatagtc cgggcaacga
     5881 tcggtgaagg cggttttgaa atcagcacaa ttacgcgagt ttcatcgtct tgcgccagcg
     5941 catctaaagc gaagagcatc gatattccac caatctcttt actgagatca tgtccgcctg
     6001 ttcccaacgc ctgagaaata cccgctccga gctggtcgat tcggcaagtc acttcctgta
     6061 gcccggtgcc tgacgcgcca ataacaccaa ttgctccgcg tttcaccaca ttagcgaagc
     6121 ctaacggaat gccgttaaca atggcggttc cacagtcagg tcccattacg atgcgctgac
     6181 gttctcgggc aagcgttttg atggattttt cctgcacgat actgacgtta tcgctgaaca
     6241 tcatcacgtt cattccgagg tttaacgctt taattgcttc ggcggcggcg tagtctcccg
     6301 gtacagaaat gagtgccagg ttggcttcgg gttcggcttc cagtgccatt tccaggctga
     6361 ccttttccgg tgttttaatg ccactatccg tttcatcatc gggtttgctg gtcagacgtt
     6421 gctgggccag tatcagtgct tcattgcaaa tatcttcttc acccatgact gcaatcacta
     6481 aatcattggg actggcgttg atttcgttac ccagacccgc atcgcgcaat tgttcgagat
     6541 tattgggcgt tcccatgacg accgaggcct gttgaatacc gggcaacttg ctgatttgcg
     6601 cggaaatctg catcagagaa actgagtcct gataaagatt actgaaaact ttatatttaa
     6661 ctttcatgct gttacgcctg tttcattccg cccgatagca aaattcgggc aaagcatccc
     6721 cgtgtagccc cataaacggg ctacgggaga aaaggttgaa atcaattcat ggtgttcagg
     6781 gttcgaaagc catgcagcat tcctgcaagg caatctactc ctgacgttgc gccaaattgc
     6841 atgacctgtt ctgcacagga tgcgattgtc attgccgatg cgctcccaac cagttgtgcg
     6901 agtaactggc aaatcggttc tgaaaaatgt ccctgaagcg cacgctccag ataatgacga
     6961 ctgatatccg tggtgttgtg cgcatgttga tcaattgcct gctgcaaccg gtattgatga
     7021 tcggcaagtg gtgcagggag ttgccataac cagagcgctg ccagatagcc caacaaatag
     7081 tcatctccat cgggcgttaa gcctttacca aagccgatca gttgtggcac ctggcgttcc
     7141 agcgcatctg gatgctccat aatcgctaat ccctggtgcg tgagactatc cggcaggtgc
     7201 aatgtgcttt gaacgttatg ctccagacaa aataaaagcc gttgcgatgc catcgttggg
     7261 tagtgctgga aaaccaaagg gaatgcctca agtgtcagca ttccccccac aaatggcgac
     7321 tgccagagcg ttgcgttatc gagcgccaca caaaatcgag gtgtccgcag caagcctggg
     7381 gttaactgaa tgggatctga atgggcaatc tctcttcgcc agtcccaaca ttcaggtagc
     7441 cagactctgg cagcatcagg gagattcaga tacgtttgcg cacacaacag agtgatgaat
     7501 gttcccgcgt ctgaatacag atttaccgca tgtttaaaag cgctatgtag acgtaaatct
     7561 tgcgtttcat cctgcgggaa aagatatccc aacgacgatg cgcaaagcgt aacctgtacc
     7621 atcggcgtta gccttccaga cctgcaataa tggctttgga atcagtaacc caaccgaaaa
     7681 tggcaccttg tgctttgatc atctcgaggg cgtatttctg gaattccggg aaataggagc
     7741 cgacacaatc ttccgggata atgcattcat agccacgatc atttgcttcg cgcacggtgg
     7801 tggttacgca aacttcggtt gttacaccac aaactatcag cgtcttgata ccgtggtttt
     7861 gcaaaatcag atgtaagtcg gtttgataaa acgcgccttt gccaggctta tcaatcaccg
     7921 gttcgcctgc caccgggtac agttcaggaa tgatgtcatg acctgcttca cctctgacca
     7981 gaatgcgtcc catcggtcct ggttcaccaa taaaggtttt tccgccgcgc gtcagtttgg
     8041 ctggcgggca gtcgcttaag tctgcacgat ggccttcacg agtatgaata accataattc
     8101 ctttttgacg tgcggctgcc agcacctctg tacatggtgc gatagccgtg cgtacccgtg
     8161 aaacatcatt gcccagtgcc tcaccgaagc ccccggcttc gacaaagtca cgctgcatgt
     8221 cgatcatgac cagtgcagtt gtgcgcggat caaaaggtaa ttcaaagggt tgagcctgaa
     8281 aaatggattg cgtcatggta aatctcctgt ctgatgttta aacgatccag ttgttctgag
     8341 aacgagtatt caggagaaaa gcggcacaaa acaggaaaaa agtttgccgc acagcaacga
     8401 aatttttgca ttcagcaggt ggtgtgatga cgaatgtgaa gggagtcaaa gagtggcata
     8461 cagcaacaaa ttttttgatg ctgtatggtt ttgagattaa aaaaatcgtt gaaattgtgt
     8521 gagcttaatc atcagattta gcgactatgg cgttactttt taacaccata ctgtgtaaac
     8581 cattccaaca ttcgctgcca gccgtctttc gcagactcgg catgatagct cggacgataa
     8641 tcggcgttga acgcatgctc tgcatccggg tacacgataa tctctgcttt cgcgtttgct
     8701 gcccgcagcg cctggcgcat cgtttccaca ctctcttgcg gaatgctgtt atcctggccg
     8761 ccatataagc cgagaaccgg cgcgttaaga tcggttgcga tatcaacagg ttgtttcggt
     8821 gaattcagcg acttatcgcc cgtcagtttg ccgtaccacg ccactgcggc ttttagctgt
     8881 ggattatgcg cggcatacag ccaggtgata cgtccacccc agcagaatcc ggtgatcatt
     8941 aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg cgacatgatc gagatcggcc
     9001 agcacctgcg agtcaggcac ttttgctacc agaccgccaa gcaacgtggg gatatcggca
     9061 aaatcattcg gatcgccttc gcggaagtaa agttcaggtg cgatagccag atacccctcc
     9121 agcgccagac ggcgacaaat gtcgcggata tgttcatgca cgccaaaaat ttcctgcact
     9181 acaatgacca ctggcagtgg gccatcgctt tgctttggtc tggcatggta agcaggcatg
     9241 ttatcccctt gtgaagggat agaggtgaag cccgccacaa tcgcgtcgtc cggagtctga
     9301 acgatggtcg aagcgagagg cgatgcagca ggtgcaaatc cagattgttg tgttgttgcc
     9361 atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt taactatagt cagcatgcaa
     9421 caaatcacat tgcctgaatc ggctcatctt ttatgcagtc ctgcagaatg aagagtgatt
     9481 tatgtgattt gcatcacttt tgatgggtaa atttatgcaa cacattttcg tcatggtgat
     9541 gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc tttttgcttc ttctgactaa
     9601 accgattcac agaggagttg tatatgtcca agtctgatgt ttttcatctc ggcctcacta
     9661 aaaacgattt acaaggggct acgcttgcca tcgtccctgg cgacccggat cgtgtggaaa
     9721 agatcgccgc gctgatggat aagccggtta agctggcatc tcaccgcgaa ttcaccacct
     9781 ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc taccggtatc ggcggcccgt
     9841 ctacctctat tgctgttgaa gagctggcac agctgggcat tcgcaccttc ctgcgtatcg
     9901 gtacaacggg cgctattcag ccgcatatta atgtgggtga tgtcctggtt accacggcgt
     9961 ctgttcgtct ggatggcgcg agcctgcact tcgcaccgct ggaattcccg gctgtcgctg
    10021 atttcgaatg tacgactgcg ctggttgaag ctgcgaaatc cattggcgcg acgactcacg
    10081 ttggcgtgac agcttcttct gataccttct acccaggtca ggaacgttac gatacttact
    10141 ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga gtggcaggcg atgggcgtaa
    10201 tgaactatga aatggaatct gcaaccctgc tgaccatgtg tgcaagtcag ggcctgcgtg
    10261 ccggtatggt agcgggggtt atcgttaacc gcacccagca agagatcccg aatgctgaga
    10321 cgatgaaaca aaccgaaagc catgcggtga aaatcgtggt ggaagcggcg cgtcgtctgc
    10381 tgaaataaaa aaacgctccc caacttcggg gagcgatatc agttattgac gccgtaaacg
    10441 cattattttg cggattattg gcgtcagtag cgtaaaggct gccagagtca gcaatgccag
    10501 agtgatcggt cgggtataca gaaagtcgag tgacccacca gaaagcgaca gcgcacggcg
    10561 aaaattactt tccgccattg gccccataat caatgccagc actacgggtg atgccggata
    10621 tcctcctttc tgcatgaaat agcccaccac gcctgcaatc agcataatgc caacatcaaa
    10681 aaggctattg ttcaacgcat aagtcccgac cacacaaagt gcgaggatca tcggtgtgag
    10741 aatattgccg ggaattttca ccacattacc gatgactttc aaagagagca gtccaagaac
    10801 tagtagcatg aagtaacaga agatcatgcc gacaaacagt gtataaacca tatcgccgtg
    10861 gtcggtaaac atcagcgggc ctggttgcat tccctgtaac gttaacgcgc ccagcatgat
    10921 ggcggttacc gcatcgccag ggatccccag cgtcagcata ggtaacaatg cgccaccggt
    10981 gcagccattc gcgcctgcct cacaggaggc cacagcttta atttcaccct taccaaagtt
    11041 ttctggtgtc ttactgaaac gacgagtttc gttataggca acaaaagctg caatatccgc
    11101 ccccgcaccg gggatcatgc cgataaaaac ccctaatcct gatgagcgca aaatggttaa
    11161 tgcgatgcgg cgtagtgttt gccagggcag taacagactg ccgatagcca ccttcgcccc
    11221 ctgacgaatt tgctggtttt gctccataga gcggaatgct tctgaagcag caaataaacc
    11281 aatcatcacc gggataaatg gcacattcat cagctccaca aatccacctg taaaccgtgg
    11341 aaaaccgccc attggatcaa ggcctatcgt tgcgatcagc aatccgccaa ccccggcaat
    11401 cagcccctta atcagcgact caccggaaat actggcaata atgctcagtc cgaaggttgc
    11461 cagggcgaag gactccgagg cactaaattt cagcgcccaa ctggcaagta ccggagagag
    11521 aaacatcagc acgataatgc tgagcgttcc gccactaaag gaagagaacg tggcggcact
    11581 caatgccagc cctgcttttc cctgcttcgt cagctcatat ccgtcaatgg cggtggctgc
    11641 ggcggcaggc gtaccgggaa ttttgagcaa aattgcggtg atagaaccgc catagacgcc
    11701 gccaaaaaag acgccgcaaa tcatcaacaa gccagaaaca ggctccatgc cgtaggtgaa
    11761 aggcagcaaa atcgccacac ccatggtcgc tgtcagacca ggcagagcgc caatcacaat
    11821 acccagcatc accccaaaag tggcaaacag cagcgcctga ggattattga gtaatgtcga
    11881 aaaaccctga gttaaaagtt cactttcaaa cattgctggc ccctgttatt cgaaaaatgt
    11941 gccgacaggc aatggcacct gtaacgcttc gacaaacagc aaccagagga ttgccgttaa
    12001 cagcagcgca atacccggtg tgagtaccca tcgcctgtag cccatcaacg tcataatgac
    12061 gatgaggaaa acaggagtag taatgagata accgcagtag ctcatcgcca caatataaaa
    12121 cgcggttgcc gcaattcctg ttgccgtttt cctgaatgcc ggtttttcct gtgcgttggc
    12181 gcaagaggct ggttttgcct gcaaaagatc gcgtatcacc agcaagacag cgagcacaat
    12241 cagggcacag gcatagaagg tagggaaggc acttgcaccg ggatccgttt caatggcagt
    12301 tgctggaaaa tcagcggcat taatcatcac cgcgccagca accacacaaa gtaacagcgc
    12361 caccaccatc gttgatgaaa ggcgcttgcg gtgcggtacg gcgagttgag caacagactg
    12421 ttttgtgttc atcaaccgta cctcaggttt attttttcag gcccaggcga gtcagcaact
    12481 cgtcaaagta cttttcctgt tcgctgattt gggtctggaa gctggcagcg tcaagccatg
    12541 catagttgag attcaacttt tgtagtgcat cctggaatgc aggctcttca gccgtctctt
    12601 ttgctgcctt tgccagaaca tccaccacat cctgcggcgt tttttgcgac acaatcaggc
    12661 cgcgccaggt gccgatggag agatcaacgc ctttctcttt taacgtcggg acgtcaggca
    12721 tggttttcat tcgctcatcc gccatcacta ccagtgtctt cagcttgccg cctttcacat
    12781 ggttgataac ttctcctggg cttacggaaa ccgcttcaat gtgcccgcct aacaggcctg
    12841 taatggcagg ggctgcgccg tcatacggga catgagaaaa ctttgcgccc gttttgtctt
    12901 ccagtgcagc tgccgccaga tgccagatag ctccggtgcc tgagttacca atgcgtactt
    12961 ttccgggatt cgctttggcg taagtcataa attcttcata gctattccac ggcgcatcgg
    13021 cacggactgt gatagcagcc ggatccgcat tcagacgggc aatgggttta aagtcgctgg
    13081 ttttaaaacg caccattccg aggctgggaa gggtggtcag ttcaaccgtg cctaaaccaa
    13141 ttttgtaacc gccagggcgg gcagcggcga tttcactcag gccgatagca ccgcctccgc
    13201 caggtttatt gataaccccg atgctgacgg gtaaatggtt tttggcggca tcagcaaagg
    13261 cacgggcaac cagatccgta ccgcctccgg cagcgtaggg aacgactaac tcgatctgtt
    13321 tggttggcca ggaggcagcc tgtacaggcg caatgctgag ggcagcgcct gcaagaagta
    13381 ccattgcagc catttttttc ggtaaggtaa gatgttttat cattgctttt atatccttat
    13441 ttatcagtta gttgctattt aaattgttcc atgcgttcat aaacagtact gcattttcat
    13501 agacttgctc tggcgtcatg ccggggtgat atagcgcaga accgaggcca aagccgtttg
    13561 cgccgtggct cagataacgc gccatggttt ccggtgtgat accgccaacc ggtaacatcg
    13621 gaacggattg tggaataacc gcacgccagg ctttggtaac ttctggcgta atttgttcag
    13681 ccgggaacag cttgatggca ttagcaccat gttcaatggc agtaaaggct tcggtgggcg
    13741 tggcgacgcc aggcgcacaa tacataccca gcgcacgggc gcgacggatc accgcagtat
    13801 cgcaatgcgg catcacgata atctggcccc cagcctcttt aaccgcaacc acctgctcta
    13861 ccgaaagcac agttccggct ccgaccaggc agtccttagg cagggcgtcg cgcagtaaac
    13921 tgatactttg caacgcctcc ggcgagttca taggaatctc aatcaggcgg aatccggcgg
    13981 cgtacagttt ttcaccgatg gcgacaatct catccggttt cacgccgcgt aaaatggcga
    14041 ctaacccggt tttttgcaaa agcgtattaa atggcatggg tacattccct ggcgttggtg
    14101 gacagtaacc cggcagcgtg ggcgattcgc cataatccct gttcagtggc attaggaatg
    14161 gcgtgtatct gtctgtcggg aaaaacgtgg ctgaatgctt gtgaatagcg gctgttcaga
    14221 tttgcgctac cgattaaggc gatcggtgtt gtggcgggta aatcacgtag caaactggat
    14281 gccagaccac ataattcatg gcctatcagt aatccggaaa ggtaatcccc ctgcgaggac
    14341 gacgtaagat tactggtcag caggcgcgaa cgggtcgaaa acagcgtcga taataatccg
    14401 gtctgatgat gctcttgtgc tgctgtcagc ccgtgggtga aggcatccca gtgcagttca
    14461 tctccaggat gcatggtggc accgagaatc gaatggcggg ataaaacatc aaataactcg
    14521 cccgtcataa aggttcggaa atcggtaatg gtgttgtttt caactactgc ccatttcgca
    14581 tgtgtgccgg gtaagcctat cagcactggc ataccctgat ggatcgcatt ttgcagggac
    14641 ggttccatcg agatagcacc gaagatttgt gtctcttcgc cacgcatcac ttcgggcatt
    14701 tcaccttctt taatcacgcc aggaatgatg tgcagcattg tctgctgctg gtggataaca
    14761 ggcgtcaact gcttcgccag accctcgaga gtgaccgggc aaggtaaata agctgctggt
    14821 tgccaccctt gcgcactgcc gagcatgccg caagccagta ccgggcatgg ggtttgtgcc
    14881 agccagtcgc cgcagacacg taaaaacgta tcgtggaaag cgtcctcacg tgttgctgcc
    14941 tgggagggta atttcatgat cccccagggg aattcacgtt tttcttgtgg atttggtgat
    15001 tcgccaaaac gccatgcccg aagcgatgac gttccccagt ccagggcaat aaatgagggt
    15061 gctcccggca ttagtcgttt cctttaatca tggtttattt atctttgcgg acgtgcttac
    15121 cccagcccag ttcagcggat atctcttctg cacaggattt gacgtaaggt gccagttctt
    15181 ccagacgcgc cagcgacata tacgtggtgg tactggcgac agaaatagcc gccacaattt
    15241 catcccgggc gttgtaaacc ggcgctgcaa cgcaacggat agtcggctcg ttctcttcca
    15301 gatcaaaagc aaaaccactg gcggcataac gacgcatgtt ctgaataaat gcgcccagtt
    15361 ttgtttcatc accacaggtc ttaaacagcg tgcgccactc ttcttctgtt cggttgagga
    15421 tcaatgcctt gccgatgccg gtgattgcca gtggcatgcg atggcctggg cgtgagcgca
    15481 tttccagccc gcgctgactg ttgatcttct ccagatacag cacttcgtcg ccttcaacga
    15541 tccccagatg cacggtgtcc agggtgtaac gggcaaggcg ttgcaaaacg ggctgggcaa
    15601 tttcgtacag cgaggtgctt tccagtgctc tggcaccgaa ttcgatcagt ttggcaccca
    15661 gttggtagcc ctgcgttggg acatggcgca ggtagcgttg ttccaccaga acggtgacta
    15721 aacgatgggt tgtgctccgg gtcgtgccgg tgaactcgcc gatacttttt aggtcatggc
    15781 agccgttata aacagcatta agaaccgcca gtccacgtaa taacgtttgt gcgcctgcgg
    15841 tggttgaaga ggtttctgcc atacgtttct cctgatgata ttgctgaagt gttccacata
    15901 atgacgtggg gaaaaggtga tatatcccac aatataaact tcatctcata ttatgggata
    15961 aaataatgaa aattaacgat cgttttgcgt agcgcctcgc aggaaacttc tttcaaactg
    16021 gacgtttgta cagcacaatt ctattttgtg cgggtaagtt gttgcgtcag gaggcgttgt
    16081 ggatttctca atcatggttt acgcagtcat tgcgttggtg ggtgtggcaa ttggctggct
    16141 gtttgccagt tatcaacatg cgcagcaaaa agccgagcaa ttagctgaac gtgaagagat
    16201 ggtcgcggag ttaagcgcgg caaaacaaca aattacccaa agcgagcact ggcgcgcaga
    16261 gtgtgaattg ctcaataacg aagtgcgcag cctgcaaagt attaacacct cgctggaggc
    16321 cgatctgcgt gaagtgacca cgcggatgga agccgcacag caacacgctg acgataaaat
    16381 tcgccagatg atcaacagcg aacagcgcct cagtgagcaa tttgaaaacc ttgccaaccg
    16441 tatttttgaa cacagtaatc gtcgggttga tgagcaaaat cgccagagtc tgaacagtct
    16501 tttgtcgccg ttgcgtgaac aactggacgg ttttcgccgt caggttcagg acagtttcgg
    16561 taaagaagca caagaacgtc ataccctgac tcacgaaatc cgcaatctcc agcaactcaa
    16621 cgcgcaaatg gcccaggaag cgatcaacct gacgcgtgcg ctgaaaggcg acaataaaac
    16681 ccagggcaac tggggcgaag tggtgctaac ccgcgtactg gaggcgtctg gtctgcgtga
    16741 agggtatgaa tatcagactc aagtgagtat cgaaaccgac actcgttcgc gaatgcaacc
    16801 ggatgtcatc gtgcggctgc cgcagggcaa agatgtggtg atcgacgcca aaatgacgct
    16861 ggttgcctat gaacgttatt tcaacgctga agatgactac acacgtgaaa gcgcgctaca
    16921 ggaacatatc gcgtcggtgc gtaaccatat ccgtttgctg ggacgcaaag attatcaaca
    16981 gctgccgggg ctgcgaactc tggattacgt gctgatgttt attcccgttg aacccgcttt
    17041 tttactggcg cttgaccgtc agccggagct gatcaccgaa gcgttgaaaa acaatatcat
    17101 gctggttagc ccgactacgc tgctggtggc gctgcgcact atcgccaacc tgtggcgtta
    17161 tgagcatcaa agccgcaacg cccagcaaat cgccgatcgt gccagcaagc tgtacgacaa
    17221 gatgcgtttg ttcatcgatg acatgtccgc gattggtcaa aatctcgaca aagcgcagga
    17281 taactatcgc caggcaatga aaaaactctc ttcaggtcgc ggaaatgtgc tggcgcaggc
    17341 agaagcgttt cgcggtttag gggtagaaat taaacgcgag attaatccgg atttggctga
    17401 acaggcggtg agccaggatg aagagtatcg acttcggtcg gttccggagc agccgaatga
    17461 tgaagcttat caacgcgatg atgaatataa tcagcagtcg cgctagccca ttgggggtag
    17521 ttaagccggg tagaaatcta gggcatcgac gcccaatctg ttacacttct ggaacaattt
    17581 tttgatgagc aggcattgag atggtggata agtcacaaga aacgacgcac tttggttttc
    17641 agaccgtcgc gaaggaacaa aaagcggata tggtcgccca cgttttccat tccgtggcat
    17701 caaaatacga tgtcatgaat gatttgatgt cattcggtat tcatcgtttg tggaagcgat
    17761 tcacgattga ttgcagcggc gtacgccgtg ggcagaccgt gctggatctg gctggtggca
    17821 ccggcgacct gacagcgaaa ttctctcgcc tggtcggaga aactggcaaa gtggtccttg
    17881 ctgatatcaa tgaatccatg ctcaaaatgg gccgcgagaa gctgcgtaat atcggtgtga
    17941 ttggcaacgt tgagtatgtt caggcgaacg ctgaggcgct gccgttcccg gataacacct
    18001 ttgattgcat caccatttcg tttggtctgc gtaacgtcac cgacaaagat aaagcactgc
    18061 gttcaatgta tcgcgtgctg aaacccggcg gccgcctgct ggtgcttgag ttctcgaagc
    18121 caattatcga gccgctgagc aaagcctatg atgcatactc cttccatgtg ctgccgcgta
    18181 ttggctcact ggtcgcgaac gacgccgaca gctaccgtta tctggcagaa tccatccgta
    18241 tgcatcccga tcaggatacc ctgaaaacca tgatgcagga tgccggattc gaaagtgttg
    18301 actactacaa tctgacggca ggggttgtgg cgctgcatcg tggttataag ttctgacagg
    18361 agaccggaaa tgccttttaa acctttagtg acggcaggaa ttgaaagtct gctcaacacc
    18421 ttcctgtatc gctcacccgc gctgaaaacg gcccgctcgc gtctgttggg caaagtgctg
    18481 cgcgtggagg taaaaggctt ttcgacgtca ttgattctgg tgttcagcga acgccaggtc
    18541 gatgtactgg gcgaatgggc aggcgatgct gactgcaccg ttatcgctta cgccagcgtg
    18601 ttgccgaaac ttcgcgatcg ccagcagctt gccgcactga ttcgtagtgg tgagctggaa
    18661 gtgcagggcg atattcaggt ggtgcaaaac ttcgttgcgc tggcagatct ggctgagttc
    18721 gaccctgcgg aactgctggc cccttatacc ggtgatatcg ccgctgaagg tatcagcaaa
    18781 gccctgcgcg ggggcgcaaa gttcctgcat cacggcatta agcgccagca acgttatgtg
    18841 gcggaagcca ttactgaaga gtggcgtatg gcacccggtc cgcttgaagt ggcctggttt
    18901 gcggaagaga cggctgccgt cgagcgtgct gttgatgccc tgaccaaacg gctggaaaaa
    18961 ctggaggcta aatgacgcca ggtgaagtac ggcgcctata tttcatcatt cgcacttttt
    19021 taagctacgg acttgatgaa ctgatcccca aaatgcgtat caccctgccg ctacggctat
    19081 ggcgatactc attattctgg atgccaaatc ggcataaaga caaaccttta ggtgagcgac
    19141 tacgactggc cctgcaagaa ctgggaccag tatggatcaa gttcgggcag atgttatcaa
    19201 cccgccgcga tctttttccg ccgcatattg ccgatcagct ggcgttattg caggacaaag
    19261 tcgctccgtt tgatggcaag ctggcgaagc agcagattga agctgcaatg ggcggcttgc
    19321 cggtagaagc gtggtttgac gattttgaaa tcaagccgct ggcttctgct tctatcgccc
    19381 aggttcatac cgcgcgattg aaatcgaatg gtaaagaggt ggtgattaaa gttatccgcc
    19441 cggatatttt gccggtcatt aaagcggatc tgaaacttat ctaccgtctg gctcgctggg
    19501 tgccgcgttt gctgccggat ggtcgccgtc tgcgcccaac cgaagtggtg cgcgagtacg
    19561 aaaagacctt gattgatgaa ctgaatttgc tgcgggaatc tgccaacgcc attcagcttc
    19621 gacgcaattt tgaagacagc ccgatgctct acatcccgga agtttaccct gactattgta
    19681 gtgaagggat gatggtgatg gagcgtattt acggcattcc ggtgtctgat gttgcgacgc
    19741 tggagaaaaa cggcaccaac atgaaattgc tggcggaacg cggcgtgcag gtgttcttca
    19801 ctcaggtctt tcgcgacagc tttttccatg ctgatatgca ccctggcaac atcttcgtaa
    19861 gctatgaaca cccggaaaac ccgaaatata tcggcattga ttgcgggatt gttggctcgc
    19921 taaacaaaga agataaacgc tatctggcgg aaaactttat cgccttcttt aatcgcgact
    19981 atcgcaaagt ggcagagcta cacgtcgatt ctggttgggt gccaccagat accaacgttg
    20041 aagagttcga atttgccatt cgtacggtct gtgaacctat ctttgagaaa ccgctggccg
    20101 aaatttcgtt tggacatgta ctgttaaatc tgtttaatac ggcgcgtcgc ttcaatatgg
    20161 aagtgcagcc gcaactggtg ttactccaga aaaccctgct ctacgtcgaa ggggtaggac
    20221 gccagcttta tccgcaactc gatttatgga aaacggcgaa gcctttcctg gagtcgtgga
    20281 ttaaagatca ggtcggtatt cctgcgctgg tgagagcatt taaagaaaaa gcgccgttct
    20341 gggtcgaaaa aatgccagaa ctgcctgaac tggtttacga cagtttgcgc cagggcaagt
    20401 atttacagca tagtgttgat aagattgccc gcgagcttca gtcaaatcat gtacgtcagg
    20461 gacaatcgcg ttattttctc ggaattggcg ctacgttagt attaagtggc acattcttgt
    20521 tggtcagccg acctgaatgg gggctgatgc ccggctggtt aatggcaggt ggtctgatcg
    20581 cctggtttgt cggttggcgc aaaacacgct gattttttca tcgctcaagg cgggccgtgt
    20641 aacgtataat gcggctttgt ttaatcatca tctaccacag aggaacatgt atgggtggta
    20701 tcagtatttg gcagttattg attattgccg tcatcgttgt actgcttttt ggcaccaaaa
    20761 agctcggctc catcggttcc gatcttggtg cgtcgatcaa aggctttaaa aaagcaatga
    20821 gcgatgatga accaaagcag gataaaacca gccaggatgc tgattttact gcgaaaacta
    20881 tcgccgataa gcaggcggat acgaatcagg aacaggctaa aacagaagac gcgaagcgcc
    20941 acgataaaga gcaggtgtaa tctgtgtttg atatcggttt tagcgaactg ctattggtgt
    21001 tcatcatcgg cctcgtcgtt ctggggccgc aacgactgcc tgtggcggta aaaacggtag
    21061 cgggctggat tcgtgcgttg cgttcactgg cgacaacggt gcagaacgaa ctgacccagg
    21121 agttaaaact ccaggagttt caggacagtc tgaaaaaggt tgaaaaggcg agcctcacta
    21181 acctgacgcc cgaactgaaa gcgtcgatgg atgaattacg ccaggctgcg gagtcgatga
    21241 agcgttccta cgttgcaaac gatcctgaaa aggcgagcga tgaagcgcac accatccata
    21301 acccggtggt gaaagacaat gaaactgtgc atgaaggcgt aacgcctgct gctgcacaaa
    21361 cgcaggccag ttcgccggaa cagaagccag aaaccacgcc agagccggtg gtaaaacctg
    21421 ctgcggacgc tgaaccgaaa accgctgcac cttccccttc gtcgagtgat aaaccgtaaa
    21481 catgtctgta gaagatactc aaccgcttat cacgcatctg attgagctgc gtaagcgtct
    21541 gctgaactgc attatctcgg tgatcgtgat attcctgtgt ctggtctatt tcgccaatga
    21601 catctatcac ctggtatccg cgccattgat caagcagttg ccgcaaggtt caacgatgat
    21661 cgccaccgac gtggcctcgc cgttctttac gccgatcaag ctgaccttta tggtgtcgct
    21721 gattctgtca gcgccggtga ttctctatca ggtgtgggcg tttatcgccc cagcgctgta
    21781 taagcatgaa cgtcgcctgg tggtgccgct gctggtttcc agctctctgc tgttttatat
    21841 cggcatggcg ttcgcctact ttgtggtctt tccgctggca tttggcttcc ttgccaatac
    21901 cgcgccggaa ggggtacagg tatccaccga catcgcgagc tatttaagct tcgttatggc
    21961 gctgtttatg gcgtttggtg tctcctttga agtgccggtg gcaattgtgc tgctgtgctg
    22021 gatggggatt acctcgccag aagacttacg caaaaaacgc ccgtatgtgc tggttggtgc
    22081 attcgttgtc gggatgttgc tgacgccgcc ggatgtcttc tcgcaaacgc tgttggcgat
    22141 cccgatgtac tgcctgtttg aaatcggtgt cttcttctca cgcttttacg ttggtaaagg
    22201 gcgaaaccgg gaagaggaaa acgacgctga agcagaaagc gaaaaaactg aagaataaat
    22261 tcaaccgccc gtcagggcgg ttgtcatatg gagtacagga tgtttgatat cggcgttaat
    22321 ttgaccagtt cgcaatttgc gaaagaccgt gatgatgttg tagcgcgcgc ttttgacgcg
    22381 ggagttaatg ggctactcat caccggtacc aatctgcgtg aaagccagca ggcgcaaaag
    22441 ctggcgcgtc agtattcgtc ctgttggtca acggcgggcg tacatcctca cgacagcagc
    22501 cagtggcaag ctgtgactga agaagcgatt attgagctgg ccgcgcagcc agaagtggtg
    22561 gcgattggtg aatgtggtct cgactttaac cgcaactttt cgacgccgga agagcaggaa
    22621 cgcgcttttg ttgcccagct acgcattgcc gcagaattaa acatgccggt atttatgcac
    22681 tgtcgcgatg cccacgagcg gtttatgaca ttgctggagc cgtggctgga taaactgcct
    22741 ggtgcggttc ttcattgctt taccggcaca cgcgaagaga tgcaggcgtg cgtggcgtgt
    22801 ggaatttata tcggcattac cggttgggtt tgcgatgaac gacgcgggct ggagctgcgg
    22861 gaattgttgc cgttgattcc ggcggagaaa ttgctgatcg aaactgatgc gccgtatctg
    22921 ctccctcgcg atctcacgcc aaagccatca tcccggcgca acgcgccagc ccatctgccc
    22981 catattttgc aacgtattgc gcactggcgt ggagaagatg ccgcatggct ggctgccacc
    23041 acggatgcca atgtcaaaac actgtttggg attgcgtttt agagtttgcg gaactcggtg
    23101 ttctttacgc tgtgcttaat ttctttattg ataagattaa gcaatagcat ggagcgagcc
    23161 tctccgtctg gctcggtgaa aatggcctga aaaccttcga atgcgccttc agtaataatc
    23221 actttatcac caggatacgg cgtcgatgga tcgacaatat ctttcggttt atacactgat
    23281 aactgatgaa tcaccgccga tgggactatc gctggcgacg cgccaaagcg cacgaagtgg
    23341 ctgacaccgc gggtcgcgtt gatagtcgtg gtatgaatca cttccggatc gaactccaca
    23401 aacaagtagt tggggaacaa tggctcactg actgcagtac gtttgccacg cacgattttt
    23461 tccagggtga tcatcggtgc caggcaattc acagcctgtc tttcgaggtg ttcctgggca
    23521 cgttgaagtt gcccgcgctt gcagtacagt aaataccagg attgcataat gactcttatc
    23581 cgtttaatcg gggcgcaagg atagcaaaag ctttacgcta agttaattat attccccggt
    23641 ttgcgttata ccgtcagagt tcacgctaat ttaacaaatt tacagcatcg caaagatgaa
    23701 cgccgtataa tgggcgcaga ttaagaggct acaatggacg ccatgaaata taacgattta
    23761 cgcgacttct tgacgctgct tgaacagcag ggtgagctaa aacgtatcac gctcccggtg
    23821 gatccgcacc tggaaatcac tgaaattgct gaccgcactt tgcgtgccgg tgggcctgcg
    23881 ctgttgttcg aaaaccctaa aggctactca atgccggtgc tgtgcaacct gttcggtacg
    23941 ccaaagcgcg tggcgatggg catggggcag gaagatgttt cggcgctgcg tgaagttggt
    24001 aaattattgg cgtttctgaa agagccggag ccgccaaaag gtttccgcga cctgtttgat
    24061 aaactgccgc agtttaagca agtattgaac atgccgacaa agcgactgcg tggtgcaccc
    24121 tgccaacaaa aaatcgtctc tggcgatgac gtcgatctca atcgcattcc cattatgacc
    24181 tgctggccgg aagatgccgc gccgctgatt acctgggggc tcaccgtaac gcgtggcccg
    24241 cataaagagc ggcagaatct gggcatttat cgccagcagc taattggtaa aaacaaactg
    24301 attatgcgct ggctgtcgca tcgcggcggc gcgttggatt atcaggagtg gtgtgcggcg
    24361 catccgggcg aacgtttccc ggtttctgtg gcgctgggtg ccgatcctgc cacgattctc
    24421 ggtgcagtca cccccgttcc ggatacgctt tcagagtatg cgtttgccgg attgctgcgc
    24481 ggtaccaaga ccgaagtggt gaagtgtatc tccaatgacc ttgaagtgcc cgccagtgcg
    24541 gagattgtgc tggaagggta tatcgaacaa ggcgaaactg cgccggaagg gccgtatggc
    24601 gaccataccg gttactataa cgaagtcgat agttttccgg tatttaccgt gacgcatatt
    24661 acccagcgtg aagatgcgat ttatcattcc acctataccg ggcgtccgcc agatgaacct
    24721 gcggtactgg gtgtagcact gaacgaagtg ttcgtgccga ttctgcaaaa acagttcccg
    24781 gaaattgtcg atttttatct gccgccggaa ggctgttctt atcgtctggc ggtagtgacg
    24841 atcaaaaaac agtacgccgg acacgcgaag cgcgtcatga tgggcgtctg gtcgttctta
    24901 cgccagttta tgtacactaa atttgtgatc gtttgcgatg atgacgtcaa cgcccgcgac
    24961 tggaacgatg tgatttgggc gattaccacc cgtatggacc cggcgcggga tactgttctg
    25021 gtagaaaata cgcctattga ttatctggat tttgcctcgc ctgtctccgg gctgggttca
    25081 aaaatggggc tggatgccac gaataaatgg ccgggtgaaa cccagcgtga atggggacgt
    25141 cccatcaaaa aagatccaga tgttgtcgcg catattgacg ccatttggga tgaactggct
    25201 atttttaaca acggtaaaag cgcctgatgc gcgtttgttt tgccctattt atcgatccga
    25261 cagagaaagc gcatgacaac cttaagctgt aaagtgacct cggtagaagc tatcacggat
    25321 accgtatatc gtgtccgcat cgtgccagac gcggcctttt cttttcgtgc tggtcagtat
    25381 ttgatggtag tgatggatga gcgcgacaaa cgtccgttct caatggcttc gacgccagat
    25441 gaaaaagggt ttatcgagct gcatattggc gcttctgaaa tcaaccttta cgcgaaagca
    25501 gtcatggacc gcatcctcaa agatcatcaa atcgtggtcg atattcccca cggagaagcg
    25561 tggctgcgcg atgatgaaga gcgtccgatg attttgattg cgggcggcac cgggttctct
    25621 tatgcccgct cgattttgct gacagcgttg gcgcgtaacc caaaccgtga tatcaccatt
    25681 tactggggcg ggcgtgaaga gcagcatctg tatgatctct gcgagcttga ggcgctttcg
    25741 ttgaagcatc ctggtctaca agtggtgccg gtggttgaac aaccggaagc gggctggcgt
    25801 gggcgtactg gcaccgtgtt aacggcggta ttgcaggatc atggtacgct ggcagagcat
    25861 gatatctata ttgccggacg ttttgagatg gcgaaaattg cccgtgacct gttttgcagc
    25921 gagcgtaatg cgcgggaaga tcgcctgttt ggcgatgcgt ttgcatttat ctgagatata
    25981 aaaaacccgc ccctgacagg cgggaagaac ggcaactaaa actttataca gtggcattac
    26041 tctacgttgc atccggcaaa cagtgcccga tgcaacgctg ccgcgtctta tcgggcctac
    26101 aaaagttctg aaccgtgggc cggataaggc gttcacgccg catccggcaa gtggttaaac
    26161 ccgctcaaac accgttgcaa tgccctgacc cagaccgata cacatcgtcg ccagaccaaa
    26221 ctggacgtct ttgtgttcca tcagattcag cagcgtggtg ctgatacgcg caccggaaca
    26281 acccagcgga tgacccagcg cgatcgcgcc accgttgagg ttgatcttct cgtcaatctg
    26341 ctccattagc ccgagatctt taatacacgg caggatctgc gcggcaaagg cttcgttcat
    26401 ctcgaatacg ccgatatcgc tggcagaaag ccccgctttt ttcagcgcca gcttcgaggc
    26461 cggaaccgga ccataaccca taatagatgg gtcacaaccc acaaccgcca tcgaacgcac
    26521 gcgggcgcgc ggcttaagac ctaattcacg ggcgcggctt tcactcatca ccagcatggc
    26581 agctgcgcca tcggaaagcg cagaagatgt acccgccgtt accgtaccgc tgacgggatc
    26641 aaacgccgga cgcagtgtgg cgagggcttc cacggtggtt tccgggcgaa tcacttcgtc
    26701 gtaattaaac tgcttcaata cgccgtcggc atcgtgacca ccggtcggga tgatttcatt
    26761 tttaaatgcg cctgactgcg tggcggccca cgcgcgggcg tgtgaccgcg cggcaaaggc
    26821 atcctgcatt tcacggctga taccgtgcat acgcgccagc atttctgccg ttaagcccat
    26881 catgcccgcc gctttggcga cattacggct caggccgggg tgaaaatcga cgccgtgact
    26941 cattggcaca tggcccatat gctccacgcc gccaaccaga catgcctgcg catcaccggt
    27001 catgatcatt cgtgctgcgt catgcagtgc ctgcatggat gaaccacaca agcgattaac
    27061 ggtaaccgcc gggacagagt gcggcacttc agccaacagt gccgcgttac gggcgatatt
    27121 aaaaccctgc tccagtgttt gctgcacaca accccagtaa atatcgtcaa gggccgccgc
    27181 ttccagtgcc gggttacgcg ccagcaggct acgcattaaa tgagcggaga gatcttccgc
    27241 acgcacgtta cgaaaagcac cgcccttcga acggcccatc ggggtgcgaa ttgcatcgac
    27301 aatgacaacc tgttccattg tgactcctta agccgttttc aggtcgccaa ccggacgggc
    27361 tggctcaacc gggggatagt acggttcgtt atgacgtgct ttattacgca gaccttccgg
    27421 tacttcatac agcgggccga ggtgctgata ttgctgtgcc atatcgaggt attttgcgct
    27481 gccgagggtg tccagccagc ggaacgcgcc gccgtggaac ggagggaagc ccaggccgta
    27541 gaccagcgcc atatccgctt ctgccggagt ggcgataatg ccctcttcca gacagcggac
    27601 cacttcgttg accatcggga tcatcatgcg ggcgataatc tcttcttcgc tgaaatcgcg
    27661 cttcggctgg ctcacttttg ccagcaggtc gtcaacgacg acatcttctt ctttcttcgg
    27721 cttacctttg ctgtcttctt tataacgcca gaaaccgagg ccgttcttct gaccaaagcg
    27781 gttggcatca aacagcgcgt cgatggcatc gcgataatct ttctgcatcc gctgcgggaa
    27841 gcctgctgcc atgacagcct gggcgtgatg cgcggtgtca atgcccacaa catccagcag
    27901 atatgccgga cccatcggcc agccaaactg tttttccatc actttgtcga tcttgcggaa
    27961 atccgcgccg tcgcgcagca actggctgaa accggcgaaa taggggaaca gtacacggtt
    28021 gacgaagaag ccggggcagt cgttaaccac aatcggcgtt ttgcccatct tgctcgccca
    28081 ggcgacgact ttcgcgatgg tttcgtcgga gcttttctcg ccgcgaataa tttctaccaa
    28141 cggcattcgg tggaccgggt taaagaagtg catcccgcag aagttttccg gacgttccag
    28201 cgcgttggcc agttcgctga taggaatggt tgaagtgttc gacgccagta cggtatccgg
    28261 gcgtactttc tgctcggttt ctgccagcac ggcttttttc actttcgggt tttcaacaac
    28321 cgcttctacc acaacatcca cgcggtcaaa cccggcgtag tcgagcgttg ggtgaattgt
    28381 ggagatcacg ccagccagtt tcagaccatc gatcttgccg cgctcaagct gcttgttcag
    28441 cagtttcgcg gcttcggtca tgccgagggt taacgacttg tcgttgatat ctttcatgac
    28501 aaccggcacg cctttccacg cagactggta agcgatgccg ccgcccataa tgcctgcgcc
    28561 cagcacagcg gcctgtttcg gggtttcaac atctttggtg agtttcttcg ctttaccttt
    28621 tacatattga tcgttaagga aaatgccgac cagtgcgcgg gcttcgttgg tatgcgccag
    28681 cgggacaaaa cttttgtttt ccaggtttaa ggcttcttca cgaccaaaac gggccgcagc
    28741 ttcaatggtt tttactgcgg tgatgggggc cggataatgt ttccccgccg tttgtgcgac
    28801 catcccttta gcgatggtga agctcatggt ggcttcaatc ttgctcagtt ttagcggttc
    28861 cagcttcggc tgacgtttcg ctttccagtc gaggtcgccg ttaatggcct ggcgtaaaat
    28921 cgccatcgcc ccttcaatca gtttttctgc tttgactaca ccatccacca gaccgatttt
    28981 cagcgcctga tccgcgccga catctttacc ggcggcaatg atttccagcg cactgtcagc
    29041 gcccagcata cgtggcatac gtacagaacc gccaaagccc ggcatgatgc ccagtttggt
    29101 ttccggcaga ccgatgcgca gatccggcgt cgccagacga taatcggtcg ccagcacgca
    29161 ttcgcagcca ccgcccagcg catagccgtt gacggcagca atggtcggca ccgggagatc
    29221 ttccaggcga ttaaacacgc tattggcaaa gtgcagccac tgacttaact gttcttcagg
    29281 aacgaggaac agggacaaaa attcggtgat atcagcaccg acgataaagg ctgctttgtt
    29341 cgaacgcagc agcagccctt ttagatctga ttgctgttcc agcacgccga tggcctcgcc
    29401 gaggctggcg acggtcgcag tgtcgagttt attaactgaa cctggggcat caaataccag
    29461 ttcggcaatg ccatcttcca gccagtcaag gtacagggtg tcgcctttgt aaagcatgtc
    29521 agtctcctga atccgcaagg tgatctggtc gtaccagatg catcgaagtg tgtattttgt
    29581 gttaaaaata tgcaaataaa agattaaaga aatgccgatc tgatcacgct cggcagaaat
    29641 cacgctctgg atgaacgatg tgctaagatg cggagactta aggtcaaaaa aacagaaggg
    29701 taaaaaatgg aatcactggc ctcactctat aaaaatcata tcgctacctt acaagaacgg
    29761 actcgcgatg cgctggcgcg cttcaagctg gatgcgttac ttattcactc cggcgaactg
    29821 ttcaacgttt ttctcgacga tcatccctat ccgtttaaag tgaacccgca attcaaagcg
    29881 tgggtgccgg taactcaggt gccaaactgc tggctgctgg tggatggcgt gaacaagccg
    29941 aaactgtggt tttatctgcc ggttgattac tggcacaacg tcgaaccgct accgacctcc
    30001 ttctggactg aagatgtaga agtgatcgcg ctgccgaaag ccgatggcat tggtagcctg
    30061 ctgcctgctg cgcgcggcaa tatcggttat atcggtccgg tgccggaacg tgcgctgcaa
    30121 ctgggtattg aggccagcaa catcaacccg aaaggggtga tcgactacct gcattactac
    30181 cgctccttca aaaccgagta cgaactggcc tgtatgcgtg aagcgcagaa aatggcggtc
    30241 aatggtcatc gtgcggcaga agaagcgttc cgttctggca tgagcgagtt cgatatcaac
    30301 attgcctatc tgaccgcgac cggtcatcgt gataccgacg taccttatag caacattgtg
    30361 gcgcttaacg aacacgctgc ggtgttgcat tacaccaaac tggaccacca ggcaccggaa
    30421 gagatgcgca gcttcctgct ggatgccggg gccgaatata acggctatgc ggctgatctg
    30481 acccgtacct ggtcggcaaa aagcgacaac gactacgcac agctggtgaa agacgtaaat
    30541 gatgagcaac tggcgctgat cgccaccatg aaagcaggcg tcagctatgt ggattaccat
    30601 atccagttcc atcagcgcat cgccaaactg ctgcgtaaac atcaaatcat caccgatatg
    30661 agtgaagaag cgatggtcga aaacgatctc accggaccgt ttatgccgca cggtatcggc
    30721 catccgctgg gcctgcaggt gcatgacgta gccggtttta tgcaggatga tagcggtacg
    30781 cacctcgcgg caccggcaaa atatccgtac ctgcgctgca cccgtattct ccagccgggc
    30841 atggtgttaa ccatcgaacc gggtatctac ttcatcgaat cgctgctggc accgtggcgt
    30901 gaagggcagt tcagcaaaca cttcaactgg cagaaaattg aagcactgaa accgttcggc
    30961 ggcattcgta tcgaagacaa cgtggtgatt cacgaaaaca acgtggaaaa catgacccgg
    31021 gatctgaaac tggcgtgatg gaaagctggt taattcctgc ggcaccggtc acggtcgttg
    31081 aagagatcaa aaagagccgc ttcattacgc tgttggcgca taccgatggc gttgaggcgg
    31141 caaaagcgtt tgttgaatcg gtgcgggcag aacaccccga tgcccgccac cattgcgtgg
    31201 cgtgggtggc gggtgcgccg gatgattctc aacagctggg tttctctgac gacggggagc
    31261 cggcgggaac ggcaggtaaa ccgatgctcg cccaactaat gggcagcggc gtcggggaaa
    31321 ttaccgctgt ggtggtgcgc tactacggcg gcatattgct tggcaccggt gggttagtga
    31381 aagcgtatgg cggcggcgtg aatcaggcgc tgcgccagct aacgacccaa cgcaagacac
    31441 cgttaaccga atatactttg caatgtgaat atagtcagtt aaccggcatt gaagcgttgc
    31501 tggggcagtg tgacggcaaa attatcaaca gtgattatca ggcattcgtt ctgctgcggg
    31561 tggcgcttcc ggcggcgaaa gtggctgaat tttccgcaaa gctggcggat tttagccgtg
    31621 gttcattgca attgttagcg attgaagaat aatccccact tcgttttgca gaactaagga
    31681 agcggcagag atgcattttc gcgccattac ccgaatcgtt ggactactgg ttatcttatt
    31741 ttcagggacc atgattatcc ctgggctggt agcactcatc taccgggatg gagcgggccg
    31801 cgcttttacc cagacctttt ttgtcgccct cgccattggc tctatgcttt ggtggccgaa
    31861 ccgcaaagag aagggtgaac tgaaatcccg tgaggggttt ctgatagtgg tgctgttctg
    31921 gaccgtgctg ggtagcgtcg gtgcgctccc ttttatcttc tcggaaagcc cgaacctcac
    31981 gattaccgat gcgttttttg aatctttctc tggcctgacc accacggggg ccactacgct
    32041 ggtggggctg gattcgctcc ctcacgccat cctcttttat cgccagatgc tgcaatggtt
    32101 tggcgggatg gggatcatcg tgttggcggt tgcgatactg cctatcctcg gcgtgggtgg
    32161 gatgcagctc tatcgcgcag aaatgcccgg cccgctgaaa gataacaaaa tgcgcccgcg
    32221 aattgcggaa acggcgaaaa ccctgtggtt gatttatgtc ttgctgaccg tcgcctgtgc
    32281 gctggcgttg tggtttgctg gaatggatgc ctttgatgcc atcggccata gctttgcgac
    32341 tatcgctatt ggcggcttct cgacacatga tgccagtatc ggttatttcg acagcccgac
    32401 tattaacact atcattgcta tcttcctgct gatctctggc tgtaactacg gtctgcactt
    32461 ttcactgtta agtgggcgta gtctgaaggt ttattggcgc gatccggaat ttcgcatgtt
    32521 tatcggcgta cagttttcac tggtggttat ttgtacactc gtactgtggt ttcataatgt
    32581 ctacagttcg gcgctgatga caattaacca ggcgtttttc caggtggtgt cgatggcgac
    32641 aaccgccggg tttacaactg acagcattgc ccgctggccg ctctttttgc cggtactgct
    32701 tttatgttca gcttttatcg gcggttgcgc cgggtcaacg ggcggtggcc tgaaagtgat
    32761 ccgcatcctg ttgctgttta agcaggggaa ccgtgagctg aaacgactgg tgcatccgaa
    32821 cgccgtctat agcattaagc tggggaatcg cgcactgccg gaacgtatcc tcgaagccgt
    32881 ttggggattt ttctccgcct atgcattggt gtttattgtc agtatgctgg cgattatcgc
    32941 cacgggcgtg gatgactttt ctgcctttgc ctcggttgtt gcaacattga ataacctggg
    33001 gccagggctt ggcgtggttg ctgataactt taccagtatg aacccggtgg ctaaatggat
    33061 cctgattgcc aacatgctgt ttggtcgtct cgaggtcttt acattgctgg tgctctttac
    33121 cccgactttc tggcgtgaat gatggagtaa tacgtgaaaa cattaattct tttctcaaca
    33181 agggacggac aaacgcgcga gattgcctcc tacctggctt cggaactgaa agaactgggg
    33241 atccaggcgg atgtcgccaa tgtgcaccgc attgaagaac cacaatggga aaactatgac
    33301 cgtgtggtta ttggtgcttc tattcgctat ggtcactacc actctgcgtt ccaggaattt
    33361 gtcaaaaagc atgcgacgcg actgaattcg atgccgagcg ccttttattc cgtaaatctg
    33421 gtggcgcgta aaccggagaa gcgtactcca cagaccaaca gctacgcgcg gaagtttctg
    33481 atgaactcgc aatggcgtcc cgatcgctgt gccgtcattg ccggggcgct gcgttaccca
    33541 cgttatcgct ggtacgaccg ttttatgatc aagctgatta tgaagatgtc aggcggtgaa
    33601 acggatacgc gcaaagaagt tgtctatacc gattgggagc aggtggcgaa tttcgcccga
    33661 gaaatcgccc atttaaccga caaaccgacg ctgaaataag cataaagaat aaaaaatgcg
    33721 cggtcagaaa attattttaa atttcctctt gtcaggccgg aataactccc tataatgcgc
    33781 caccactgac acggaacaac ggcaaacacg ccgccgggtc agcggggttc tcctgagaat
    33841 ctcaacagag aaaagcaaaa aaatgcttga ctctgtagcg ggaaagcgta ttatgcacac
    33901 cccgcgccgc tgagaaaa
//
LOCUS       DACADF010000039        33108 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.039, whole genome shotgun sequence.
ACCESSION   DACADF010000039 DACADF010000000
VERSION     DACADF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.039"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(182..472)
                     /gene="ilvN"
                     /locus_tag="ISB30_001762"
     CDS             complement(182..472)
                     /gene="ilvN"
                     /locus_tag="ISB30_001762"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAP1989401.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(476..2164)
                     /gene="ilvB"
                     /locus_tag="ISB30_001763"
     CDS             complement(476..2164)
                     /gene="ilvB"
                     /locus_tag="ISB30_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAP1989402.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVSASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFSIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKHPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(2270..2368)
                     /gene="ivbL"
                     /locus_tag="ISB30_001764"
     CDS             complement(2270..2368)
                     /gene="ivbL"
                     /locus_tag="ISB30_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAP1989403.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            2769..3953
                     /gene="emrD"
                     /locus_tag="ISB30_001765"
     CDS             2769..3953
                     /gene="emrD"
                     /locus_tag="ISB30_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAP1989404.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVSTSSLMVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(3961..4458)
                     /locus_tag="ISB30_001766"
     CDS             complement(3961..4458)
                     /locus_tag="ISB30_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP1989405.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(4455..4817)
                     /locus_tag="ISB30_001767"
     CDS             complement(4455..4817)
                     /locus_tag="ISB30_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAP1989406.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(4807..5154)
                     /locus_tag="ISB30_001768"
     CDS             complement(4807..5154)
                     /locus_tag="ISB30_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAP1989407.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLFIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(5214..6708)
                     /locus_tag="ISB30_001769"
                     /pseudo
     CDS             complement(5214..6708)
                     /locus_tag="ISB30_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(6705..8420)
                     /locus_tag="ISB30_001770"
     CDS             complement(6705..8420)
                     /locus_tag="ISB30_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP1989408.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKTAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            8587..9453
                     /locus_tag="ISB30_001771"
     CDS             8587..9453
                     /locus_tag="ISB30_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1989409.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(9543..11204)
                     /locus_tag="ISB30_001772"
     CDS             complement(9543..11204)
                     /locus_tag="ISB30_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAP1989410.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLHLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(11402..11830)
                     /gene="ibpB"
                     /locus_tag="ISB30_001773"
     CDS             complement(11402..11830)
                     /gene="ibpB"
                     /locus_tag="ISB30_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAP1989411.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(11942..12355)
                     /gene="ibpA"
                     /locus_tag="ISB30_001774"
     CDS             complement(11942..12355)
                     /gene="ibpA"
                     /locus_tag="ISB30_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAP1989412.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            12661..12993
                     /locus_tag="ISB30_001775"
     CDS             12661..12993
                     /locus_tag="ISB30_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAP1989413.1"
                     /translation="MIKNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEADAQETNNVI
                     PPAKMPAN"
     gene            complement(12997..14244)
                     /locus_tag="ISB30_001776"
     CDS             complement(12997..14244)
                     /locus_tag="ISB30_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAP1989414.1"
                     /translation="MAGTVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPS
                     GASFTGETIERVNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYY
                     DFHHRRGVIVESGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMH
                     ELDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYE
                     EGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEAGWKVAGDAPLSGTETTLPAPPR
                     GVVQRRLTFTHHRASPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEP
                     RQLTHNKTDIQSAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVV
                     FSPDGQWLAWMEDGQLWITETDR"
     gene            14312..15412
                     /gene="cbrA"
                     /locus_tag="ISB30_001777"
     CDS             14312..15412
                     /gene="cbrA"
                     /locus_tag="ISB30_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HAP1989415.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(15373..16710)
                     /locus_tag="ISB30_001778"
     CDS             complement(15373..16710)
                     /locus_tag="ISB30_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1989416.1"
                     /translation="MVSGFAMPKIRRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFIGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(16785..17933)
                     /gene="dgoD"
                     /locus_tag="ISB30_001779"
     CDS             complement(16785..17933)
                     /gene="dgoD"
                     /locus_tag="ISB30_001779"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP1989417.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(17930..18547)
                     /locus_tag="ISB30_001780"
     CDS             complement(17930..18547)
                     /locus_tag="ISB30_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAP1989418.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPNIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(18531..19409)
                     /locus_tag="ISB30_001781"
     CDS             complement(18531..19409)
                     /locus_tag="ISB30_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAP1989419.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSIPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENAVDAFAAGLERGLNAPAVLPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQQVITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRNIAHAVAN"
     gene            complement(19406..20095)
                     /gene="dgoR"
                     /locus_tag="ISB30_001782"
     CDS             complement(19406..20095)
                     /gene="dgoR"
                     /locus_tag="ISB30_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAP1989420.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            20374..21030
                     /locus_tag="ISB30_001783"
     CDS             20374..21030
                     /locus_tag="ISB30_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989421.1"
                     /translation="MKLNFKGFFKAAGLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVNMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(21074..21886)
                     /gene="yidA"
                     /locus_tag="ISB30_001784"
     CDS             complement(21074..21886)
                     /gene="yidA"
                     /locus_tag="ISB30_001784"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP1989422.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(22000..22407)
                     /locus_tag="ISB30_001785"
     CDS             complement(22000..22407)
                     /locus_tag="ISB30_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAP1989423.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQSNQSVSGEQVESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(22542..24956)
                     /gene="gyrB"
                     /locus_tag="ISB30_001786"
     CDS             complement(22542..24956)
                     /gene="gyrB"
                     /locus_tag="ISB30_001786"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP1989424.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(24985..26058)
                     /gene="recF"
                     /locus_tag="ISB30_001787"
     CDS             complement(24985..26058)
                     /gene="recF"
                     /locus_tag="ISB30_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP1989425.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(26058..27158)
                     /gene="dnaN"
                     /locus_tag="ISB30_001788"
     CDS             complement(26058..27158)
                     /gene="dnaN"
                     /locus_tag="ISB30_001788"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP1989426.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(27163..28566)
                     /gene="dnaA"
                     /locus_tag="ISB30_001789"
     CDS             complement(27163..28566)
                     /gene="dnaA"
                     /locus_tag="ISB30_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP1989427.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            28863..28943
                     /locus_tag="ISB30_001790"
     CDS             28863..28943
                     /locus_tag="ISB30_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989428.1"
                     /translation="MTIDKKWLNRSNKDPGRSLRFTRQPV"
     gene            29173..29313
                     /gene="rpmH"
                     /locus_tag="ISB30_001791"
     CDS             29173..29313
                     /gene="rpmH"
                     /locus_tag="ISB30_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP1989429.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            29330..29689
                     /gene="rnpA"
                     /locus_tag="ISB30_001792"
     CDS             29330..29689
                     /gene="rnpA"
                     /locus_tag="ISB30_001792"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP1989430.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            29653..29910
                     /gene="yidD"
                     /locus_tag="ISB30_001793"
     CDS             29653..29910
                     /gene="yidD"
                     /locus_tag="ISB30_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP1989431.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            29913..31559
                     /gene="yidC"
                     /locus_tag="ISB30_001794"
     CDS             29913..31559
                     /gene="yidC"
                     /locus_tag="ISB30_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP1989432.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            31665..33029
                     /gene="mnmE"
                     /locus_tag="ISB30_001795"
     CDS             31665..33029
                     /gene="mnmE"
                     /locus_tag="ISB30_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP1989433.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         7942 a         8903 c         8454 g         7809 t
ORIGIN      
        1 agtttttaga gaaatctatc aacactgctc caggtatttg gggccgtgaa gtgaattggc
       61 ctgatgatat gaataagaaa tcaatgttgt cttcaatgga cgaatatata ctctgaataa
      121 tttgagttgc agaatataag ctcgcccgga tgtaaatatc ccatccaggc gagaaaatca
      181 cttactgaaa aaacaccgca atcttgttaa acatcgtcgg gtcggactga ttacgctgca
      241 ctttcaccac atcttccagc ttgtcgattt ggcttatcat ctgctccaga cgctggtcgt
      301 cattgaccag tagccagatg tggcttttgt cgctgtcctg aatcggcagg cagagaatgc
      361 cttcaacgtt aaaagcgcgg cgggcaaaaa ggccacaaac gtgggtcatt acacccggat
      421 gattgcgaac ggtgagctcc agaatcacgt tatcatgagt tgtgttttgc atggcttatt
      481 cccccaccat ttcagtattc gccgcacctg gcggcaccat cgggtacact ttttcttcgg
      541 catcaatgcg cacatggatc agcgccgggc cagggcgatt gatgatttcc tgcaatgcag
      601 cctgcggatc ggcttcgtta ttcaaatcac aggtttcgag gccgaatccg gcggcaatct
      661 gcataaagtt gattttgccc ggataggtag cggcaaaaac gccctgcttg tagaacagac
      721 tctgttgctg atgcaccagc cccagcgctt cgttgttcat caaaatgatt ttaacatcca
      781 gctgattttc actggcggtc gccatctcct gaatattcat catcaggctg ccgtcgccgg
      841 agaagcacaa cactttgcgg tccgggttcg ccagcgccgc gccaatcgcc gcaggcaggc
      901 caaaacccat cgtgcccagc ccgccggagg tcagccactg gcgtgggcga ttgagcggat
      961 aggcttgcgc ggtccacatc tgatgctgac ccacgtcggt agtgataatc gcattgtcat
     1021 cgacacaggc ggcaacggcg ttgatcaggc cgtaatggct taatggatcg cacgctttcg
     1081 ggattggaca cgggaattca cgctgcaaat ccgccaccaa ctggtgccac tctgcacgcg
     1141 gttgcgcttc caccagcggg atcaactgcg ccagcacgtc atcaacatcc gcctgaattg
     1201 ccacatgcgg ttgcttgatt ttgcccagct ctgcgcggtc gatatcgaca tgaataattt
     1261 tggcattcgg acagaactgc tctgttttgc caatcgcccg gtcatcaaaa cgcgcaccga
     1321 gtacaatcag cagatccgcc tcctgcaaaa tatagttggt gctgcgcaca ccgtgcatcc
     1381 ccagcatacc cagcgacagc ggatgcgctt ttggcaacat acccagcgcc attaaagtca
     1441 tggtggtagg cagttgcgct ttctccgcca gttcacgcac tcgcgcaggc gcgttgatca
     1501 caccaccacc cagataaagc accgggtgtt tggcggcgtt aatcatcgcc gccgcgtcac
     1561 gaatactttc ttcgctaaag gcgggtgcgg cggctttttc tgccatagcg ggctgtgttt
     1621 caatctcaaa aactgccgtt tgcacatcct taggaatgtc tatccacacc gggccagggc
     1681 ggcctgattg cgcaatgctg aaggcatcgc tcatgacctg cgggagttct tcgatatgtc
     1741 tgaccagata gttgtgtttg gtgatgggga tagagatgcc gtaggtgtcg acttcctgga
     1801 aggcgtcggt gccgatcatc gaggcggaaa cctgaccagt gatgcaaatc agcgggatgg
     1861 agtccagcct cgcatcggca atggcggtca ccaggttagt cgcacccggt ccgctacagg
     1921 ccatacagac cgccggttta ccgtcggtgc gcgccattcc ctgggcgata aaccccgccc
     1981 cttgttcgtg gcgagccaga atatggcgga tttgcgtgct ttggcttaag gcatcgtaaa
     2041 caggcaggat agaaccgccc ggaatacccg tcacaatctt aatgccctgc tgttccagga
     2101 aatgaacgat aaattctgcg ccggtaaagc gcttacgcgt cgatgttgtg cccgaacttg
     2161 ccatgctcca gtccttttct tctgggccga ctttccggga ggtgcttaaa cgaaaaaccc
     2221 cgcccggttt gcgccggcgg ggttttggaa tcgtgtgttg ttccagtccc tacggcgcat
     2281 tgccgacgac caccaccaca cgcacgacga ccactgcggc ggatggcgca gttggtagta
     2341 gttttgcgtt gaacatggaa gtagtcatta gggaccttgt tggttttgtg tttaacaata
     2401 tttatacaag cacagcttta caggggagac aatggaaaat ttttcttccg gataaaaata
     2461 cgtggcagat cacattttga actgaaactc aggcaataaa gcatcatcac ctgaaagacg
     2521 gtcaggttgt tgacagagtg cgccttattc atgtcgaata cagcgtaaac actttactct
     2581 accaaccgca ccaccactgt ttccggcaac atttattgcc gcttttgttt acatattctg
     2641 ccgctaaaca attccccatt cctggcgtat atctggctaa cattcatcaa tgtgatagat
     2701 tcctctcccg catttatggg aatgcgtagt gacttattct aattattttt ataaaagcat
     2761 ccgtgataat gaaaaggcat agaaacgtca atttgttatt gatgttggta ttactcgtgg
     2821 ccgtcggtca gatggcgcaa accatttata ttccagctat tgccgatatg gcgcgcgatc
     2881 tcaatgtccg tgaaggggcg gtgcagagcg taatgggcgc ttatctgctg acttacggtg
     2941 tctcacagct gttttatggc ccgatttccg accgcgtggg tcgccgaccg gtgatcctcg
     3001 tcggaatgtc catttttatg ctggcaacgc tggtcgcggt ctcgacctcc agtttgatgg
     3061 tattgattgc cgccagcgcg atgcagggga tgggcaccgg cgttggcggc gtaatggcgc
     3121 gtactttgcc gcgcgattta tatgaacgga cacagttgcg ccacgctaac agcctgttaa
     3181 acatggggat tcttgtcagt ccgctgctcg caccgctaat cggtggtctg ctggatacga
     3241 tgtggaactg gcgcgcctgt tatctctttt tgttggttct ttgtgccggt gtgactttca
     3301 gtatggcccg ctggatgccg gaaacgcgtc cggtcgacgc accgcgcacg cgcctgctta
     3361 ccagttataa aacgcttttc ggtaacagcg gttttaactg ctatttgctg atgctgattg
     3421 gcggtctggc cggaattgcc gcctttgaag cctgctccgg cgtgctgatg ggcgcggtgt
     3481 tagggctgag cagtatgacg gtcagtattt tgtttattct gccgattccg gcggcatttt
     3541 ttggcgcatg gtttgccgga cgtcccaata aacgcttctc aacgttgatg tggcagtcgg
     3601 ttatctgttg cctgctggct ggcttgctga tgtggatccc cgactggttt ggcgtgatga
     3661 atgtctggac gctgctcatt cccgccgcgc tgttcttttt cggtgccggg atgctgtttc
     3721 cactggctac cagcggcgcg atggagccgt tccccttcct ggcgggcacc gctggcgcgc
     3781 tggtcggcgg tctgcaaaac attggttccg gcgtgctggc gtcgctctct gcgatgttgc
     3841 cgcaaaccgg tcagggcagc ctgggattgt tgatgacctt aatgggattg ttgatcgtgc
     3901 tgtgctggct accgctggcg acgcggatgt cgcatcaggg gcagcctgtt taagctcacg
     3961 tcaccgcagc atcgtcatca gctccatggg agaacgatgc tgctttatca gatcacgcat
     4021 cacccgcata tgcggcgcgg agtaagaata aaacgcctga tagcccgcac aaagcacgtt
     4081 gtctggttta tcttcccgag cgtgcgggca caggcgatgc cagtcgcacg cctggcagtc
     4141 acggcgcggt tgcgactttt cgctgccaca ccatttttgt aaggtggtgt caaaaagttc
     4201 aatcgaaata cgactgatat ctgcccgcac ccaacactcg aacagggcaa ttaaaaattt
     4261 cccccacagc tctgctgtca ccgaagcggc cgtcagatcg ccccggtcat caaattcaag
     4321 atggggaata agctgtactg agcggatgcc cagctgtttg aaattgtgat aaaaatcggc
     4381 aggttgcaga gcatccttat actctacaac cagtttatgc ctgtcctctt cagcatcaat
     4441 gacctgactt cctgtcatgc gactctctca ataaatacga taagttgatg tatatgcgtt
     4501 acagcaaaca gtattgcgag agataacacc gcgagggaga tcaaaaattt gtcacggact
     4561 acgtgaaatt gggaaaaatc gctattcgtg acatccatta aattgcgatt acgggtgtag
     4621 tgccagagga tcagcgccac aatggcgagg atgccaatcg aaatccagaa taacatgccc
     4681 gcctggtgcc agttgtgttt gatagccaac gccatcaacg cgccgtagcc cagcatggta
     4741 cgaaaccacg ccagtgatgt gcgttccggc tgcagccctg ggtcggcaat gcgtctggct
     4801 ttgcggctat ccggcataca acaccagtcc catgacgatc actgccacga ccatcaatat
     4861 taagctgatg atgaataagc tgttggtata gggcaggtcc tctttcaggc gcatcgcttt
     4921 ttcattgcgt aaccagcgca agtagccata catcgctaac ccgccggaaa acagacacaa
     4981 caacaacgcc agcagttcgc ggatgacggg cgtggcgaaa tctggcgcaa gttgatcaag
     5041 cccaaccccc gccgccagaa atcctagtgc agtacggatc cacgccagaa aagtacgctc
     5101 attagccagc gagaagcggt aatcgggcgc ttctccgagg cgggaaattt tcattgttgc
     5161 tccttgtaaa ctgttcctcg ccgaaaaatt aggccaggaa cagcaataat gcatcagaac
     5221 ttctgttttt tctcttccac cttcacccct tgtgtcggta aaccggtgtc atagtcgcgt
     5281 accactggag aataaccatc ctgtgggcgt ggacgaaacg cccccatcca gcgcggttgt
     5341 gcatctttgc gccacggacg caggttccat tgataactgc ggaacggatc gcgaattttg
     5401 tccatgtaat ccaataaggc gtcatgcatt ttgctgcgaa cgtcggcaaa atggatatca
     5461 tcgatcaggt tatgcatttc atttgggtca ttacggcgat cgtaaagttc atcactggtg
     5521 aagaggttga gcaccagttt aaagtcatcc gttacccagc aacgcaccgg aataaaaccg
     5581 ccaaagctgt catgctcaat ctcgtagcga ttaaattcca ccaacacgcc acgtggttct
     5641 tttacggcaa ggatattttc ccccggcaga atctctggtt tttcaatatc tgccagcgcc
     5701 atcattgtcg gcagtaagtc aatatgactg actggcgtat cgacctgtcg ccgctcccct
     5761 tgcggcgaac ggatgatcag cggaatgcgg gtgatgtcgt catacatcgc cgctccttta
     5821 ctgatcagct tatgtgcgcc catcatttcg ccgtgatcgg aggtataaat aacccacgta
     5881 ttttcacgtt gctctggcgt taaggcgttg atgacccgtc cgatttggtc atcaacaaag
     5941 tcattgcagg caaaatagag cggatggtga taaagcccgt catcaccgac tggcgatggc
     6001 atcgcctgcg cccataagcg gtgatgttcc ggtttgttcg ccaggtcatc ctctgctttc
     6061 tcacccagtt cgtagtaaaa atcagtgtat ttctctaaat actccaccgg acaggtgaac
     6121 gggtgatgcg gctcatcata cgaaatcacc atcaggaaag gttcgtcggc gcgcgcgggc
     6181 tgttgcagaa aatccaccgc ccgattgctg atgcggtgcg cccaggtgaa ggtttcgtca
     6241 atatggttcg cctgtaaatc ttcgacgctg tttaggccat tgcgccacag gctaatctct
     6301 ttttccgtca gttcactaag atagttcgcc ccatcgaacc agtaatcagc gtcccactcc
     6361 ggcggacact cgccaggtgc cgaaatagtc atgaccgtcg agatgccatt tgccgatgta
     6421 acaggtgtga tagccggcat ccttaaagta gcgccccata gtggagatgt ttttgcctgg
     6481 cgcgacgttg ttggtccacg ggccggactg gttagcgtag ataccggtga atagtccggc
     6541 gcgtgcaggc gtacaaaccg gtgaacaggt gtaggcggag ttaaagcgaa taccttccgc
     6601 cgccagacta tcaatatttt gcgtattcag cggcttaccg ctatagcaac cgaccatatt
     6661 ggtggcctgg gtatcggtca tgatgaacag aaaattgggg cgtttcatag gctatccttt
     6721 gcatcgggag taaaggttac ggctgggtcg tgacgatgcc gccagctgtc aaaaatcagg
     6781 tagacaatca ctacggcggc aatgaaataa ctgatcatcg ctaaccagcg cgtaccgtag
     6841 ccgccgaatt cagccagccc ggcatagacg ccaatcatcg cgaacaggat accaattgac
     6901 gcagtcttga cgtttttcca cggtttcatg tccaccgcaa acgcatcttt gaaggtgaac
     6961 ggcgtggcgc gcggtttgat aaaaccgatc accagcatca cgaccacgtt gatgcagaac
     7021 gtacaggcca gcacatagag gaaatggaag tcgaacttca ccagatagtt gatggtgatg
     7081 tagctgatta tgccaatccc catcgccact tttgccgcca gcgccggaat gcgcgggaag
     7141 aaaaagccca taatgatgat ggtaaccagc ggcacgttgt aaataccgtt gagctgtttc
     7201 atccagctat acagcccctg cggcgcgttg gcgatccacg gcgcgaccat caccgaaacg
     7261 atggcgataa agaaaccaaa tttgcgcccg acggtgacca gctgttgcgg ctcggcattc
     7321 tggttaatga tacgccggta aatacccata ctgaataacg tactggcgct attcagaaag
     7381 ccgttgaagg tactgatcac cgcaccaaat aacaccgcac cgaagaagcc caccagtggc
     7441 accggcagaa cgttattgac cagcgtcggg taggccatgt cggctttcgg taaatcctga
     7501 tacagatgaa acgcaatcaa ccccggcagc accagtacca gcgggtcgag cattttcagc
     7561 accgccgtta acagtgcccc cttttgccct tccgccaggc tttttgacgc cagcgtgcgt
     7621 tgcacgatgc cctgattggt acaccagtaa aaggtgttaa ccagaatcaa accggtaaat
     7681 gccgcgccaa tcggcaaggg atcggtcggg ccaccgactg agtttaattt ctcggcgtga
     7741 acggtggtaa gttgctcaat gccctgcata aagctgccct tgcccatcgc gatcaggcca
     7801 aataccggca ccatcaaccc gccaataacc agcccaatac cattgatgga gtccgccact
     7861 gccattgcgc gcagtccgcc gatcaccgca tacaaaattc ccgccagacc aagcaaaata
     7921 accagtaacc agatagccgc accatcggaa agttgtagcg actcacccac atgaaacagg
     7981 ctgttgagcg ccaacgcgcc ggaatagaga acaatcggca gaaagcagac gccggtggca
     8041 atgaggaagc agaagtcgat gataatacgc gtcgttttat cataacgttc ctccagaaaa
     8101 tcggggatgg tggcaatgcc gcgcttcaga tagcgcggta aaaagattag cgcgaggaag
     8161 atcagcgtca ctgcagaggt gacttcccag cccatcaccg acatgccgct tttgtaggcc
     8221 tgcccggaaa ggccgaccag ttgttccgtg gaaaggttgg ttagcattaa cgaagcggca
     8281 ataaccggcg cttttagtga acgtccggca agaaaatagc cttgttgcga tccggtatct
     8341 gttttacgga ccttccacca ggtgatgacc gccaccagca gcgtaaaacc gacaaaactc
     8401 aagatttgta acgaattcat ctcatagccc ttatttatgt ttatgtcccg gacgcatgaa
     8461 tatagggaga ggtaaaatgc gtcgggtcag gcagtcagat tcttaaaata caaacgtcgt
     8521 atccctgaac ggattgtttt ctgttaagtt caggttgtga gcataattcg ccggagggat
     8581 attaaaatga atggaaaatt gcaaagttcg gatgtaaaaa acgaaactcc gtacaatatt
     8641 cccttattga ttaacgaaaa tgtgatctcc agcggaattt ctctgatatc gctctggcat
     8701 acctacgccg acgagcatta tcgggtgatc tggccgcggg acaagaagaa accgcttatc
     8761 gccaactcat gggttgcggt gtataccgta caaggatgtg ggaaaattct tttaaagaat
     8821 gacgaacaaa taactctgca tggcaactgt attatatttt taaagccaat ggatattcac
     8881 tcttatcact gtgaaggttt ggtctgggaa cagtactgga tggaatttac ccccaccagt
     8941 atgatggata ttcccgtcgg tcagcaaagc gttatttata atggcgaagt ttataatcag
     9001 gaacttaccg aagttgctga gttaattact tcaccagaag caataaaaaa taatctggca
     9061 gtcgcttttc tgacgaaaat tatttatcag tggatttgtc ttatgcacgc agtcggtaaa
     9121 aaagatccac aacggcggca aattgaaaaa ttaattgcca ctttacatgc cagtctacaa
     9181 caacgctgga gcgtagctga tatggctgcc acgatcccct gtagcgaggc ctggttgcgt
     9241 cgtctgtttt tacgctatac cggcaagacg ccgaaagaat attacctcga tgcgcgtctg
     9301 gatctggcgc tatcgctatt aaagcaacaa gggaactcgg ttggcgaagt cgctgatacg
     9361 ctcaacttct tcgactcctt tcatttcagc aaagccttta agcataaatt tggttatgcg
     9421 ccgtcagctg tgctgaagaa tacggacagg taataaccag aatggcgagt gtgtaaatga
     9481 ctcgatgatg ttcagaaata ccaggcggag taccatttac ggcgactcca ccgggaaaag
     9541 agttaaccga tactccagaa gagcaccgcc agtaattggg gtgtgataat gcgcaggaac
     9601 atcactaacg gatagacagt ggcgtaagag agcgccgctg caccgctggt tggatgaaga
     9661 ttattagcaa acgccagcgc cggaggatcg gtcatggaac ctgccagcat cccgcacatg
     9721 gtcaggtaat tcattttggc taacatccgc gccagaatac caacagtaat cagcggaacg
     9781 gcggtgatca gggcaccata accaatccag cttagccctt cgccatccac cagtgtatgg
     9841 ataaaatcgc cgccggattt caggccaacg accgagagga acagtacgat ccccagctcc
     9901 cgcagcgcga ggttggcgct tggcggcata aaccagtaca gcttgccgat actgccgata
     9961 cgcccgagaa tcaacgccat aatcagcgga ccgcccgcca gccccagttt caacgcggcc
    10021 ggaaatcccg gcacaaagac gggaatagaa cctaacaata cgccaagccc gataccaata
    10081 aacactggca gcatctgaac ctgttgcagt ttttgctgcg cattccccag cacattggca
    10141 acggcatcaa ttgcggacgg acgccccacc aggttgagga tatcgccgaa ctgcaggctg
    10201 atatcgctac ttgcaactaa ttcaacacca gcacggttca ggcgcgagat aacaacgtca
    10261 tagcgttctt taaagtgcag gtcgcgaata cgtttaccga gcacgttttc attggtgacc
    10321 accacacgct cgacgcgcaa atcagtgcct ttcgtagaca gcgaggtatc gacctcctga
    10381 ccaatcacca gttgcgcatt atgtaaatcc gcaggctgcc ccaccagatg cagcaaatcg
    10441 cccagttgaa taacggtttc tggcgaaggc acttttaggg tttcttctcg tttcaatcgt
    10501 gagcaaataa ctttatcgcc attgagaatc ggcacatctt taatggctaa attatgcaaa
    10561 ttaggattct caactcggat gttaatcgtc ctgattagcg caccgccgtt ggttcgcgtg
    10621 gactcgtgct gctgggcctc agtctcaaca ttgacgcgga aaataacccg cagcatccac
    10681 atagtgaaca aaatgccgca aatcccaaat ggatacgcca tcgcatagct catccccatt
    10741 tgatcgacca tcgccatcgg tgtccccagg tcgcgcaaga tctgctgccc tgcccccagc
    10801 gctggcgtat tggtgaccgc gccggaaaaa atccccagca cgaccggcag tggaatatca
    10861 aacagtttat gcaggatggc ggtaaccaga ccaccgatga tgacgatcag aacagcaaac
    10921 aggttgaggt gtaatccgga gacgcgcaat gaggcaaaga agcccggccc tacctgaatc
    10981 ccgatggtat aaacgaacag gatcaggcca aattcctgaa taacatgcag catatcgcta
    11041 cttaatgtca tccccgcctg agaaacaaaa tggccgacga tgatcccgcc gaacagcacg
    11101 ccgccaatgc ccaatccaac accgcgaaac ttgacgttgc cgataaacaa cccgacgacc
    11161 gccaccaaag ccagaatact gaccgttaat gctatatcac tcatcgtcca tttctcttga
    11221 aataacattt taattgctac ggattatggc tgaatcggtg aagcagattt gccttatagc
    11281 gcacattatc acgggaaaga acgtgccgaa atatcttaaa caggcgaaat ttaacgcatt
    11341 gttgaagaaa aaagacaaaa aaaaagcccc accagaatgg cggggcaaag agaatagcta
    11401 gttagctatt taacgcggga cgttcgctga tagcgatacg ctgcgctgcg atgggttcag
    11461 gctcattacg aattaaatca atatgcagta aaccgtttac gaaggttgcg ccagagactt
    11521 ccatattttc agccagcgta aagctcaggc taaaaggctg attcataagc ccttgatgca
    11581 gccatttttt ctcttctttt ggctgctccg gtgtgccttt tacgctcagg cgcgtacctt
    11641 ccagttgaat ctctaaatct tcctgacgaa aacctgccag cgcgagggta atgcggtagt
    11701 ggttatcgtc gcttttctca atgttgtacg gcgggaagct ctggctttca ccggcgtttt
    11761 gcagtgcgtt ggccagtttg tcaaaaccga tccattgacg catcagtggg gataaatcga
    11821 agttacgcat agtcatttct ccttctaaga agcgagtaag tacctgcaaa tccgaagatt
    11881 cgcatatgct ccctgacggc gagcatggag atgtcaggcc gcgccaggcg gccttaggga
    11941 attagttgat ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga
    12001 tatacagcag accattcacc aggttagcac cacgaacatg aatgttctca gctaactgga
    12061 atttacgttc aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc tctttttgtt
    12121 cgtcggcgtg agcacctttc accaccagca gattatcctg ggcggtaatt tccagttcgc
    12181 tctcagcaaa accagccaca gcgatagcaa tgcggtaatg gttttcgtct accagttcaa
    12241 cgttatacgg agggtagccg ccattactct ggctctggtt gttttctaag tggttaaaca
    12301 aacggtcaaa tccaatagca gaacggtaaa gcggggataa atcaaagtta cgcataatca
    12361 atagctcctg aaatcagcga gaatctaaga ccttccacaa tggacaggtc aggtagccag
    12421 aacacccatc aggcgcgtcc tcatcggcta cgatgtaaaa atgggtctgg aaatgaactt
    12481 ttcaagcctt atcagcggac ttttttttgc agtttatggt ctattgcata gactgagggg
    12541 gcagcacgaa acgttaaagt gcgatagccg ccacagtgcg acgtaatgat gatgttattt
    12601 tcagcaaggg atcgctataa ctcatcaggc caacacccac gatcaacaga tgaatttatt
    12661 atgataaaaa atgtgttgtt agcgttcatg atatgcagcg gaatgacatt actcggagga
    12721 tgctccagcg tgatgtccca taccgggggg aaagaaggaa cgtatcctgg cacccgcgcc
    12781 agcgcaacca tgattggcga cgatgaaacc aactggggca caaaatcgct ggcgatactc
    12841 gacatgccat ttaccgcagt actggacaca atattgctac cgtgggatgt gttccgcaaa
    12901 gacagctcag tgcgctcgcg cgttgaaaaa agcgaagccg acgcgcaaga aaccaataac
    12961 gtcattcccc ccgccaaaat gcctgcaaac tgagagctag cggtcagttt cggtgatcca
    13021 caactgaccg tcctccatcc acgccaacca ttgaccatcc ggcgagaaaa ccacggcgtc
    13081 cgcagaaggt ggattggcgt ggttttcggt taaatactca acctcgccgc tttgcgcatg
    13141 ggcacaagca atccgattat ccagcacaaa tcccagccat tcgcctgatg gatgccagtt
    13201 aaatgcagac tgaatatccg ttttattatg ggttaactgg cgtggttcac cgccctgtgg
    13261 cgagataagc cacagttgca caatgccgtt atcatcacgc attaaaaacg cgatttgcgt
    13321 accctgcgga ttacagcgca cccagtggcg cgggacgttg actaatcccg gagaagcccg
    13381 atggtgcgta aaggttaaac gtcgctgcac gacaccacgc ggtggcgcgg gcagggttgt
    13441 ttccgttcca cttaacggcg catcacctgc cactttccag ccagcttcat cttgcggcaa
    13501 ctcgacaata aacagctccg gcactttctc gccctttggc gaaagtgtgt cgccaataaa
    13561 cgccagcgcg tgatttccta cccatccttc ttcataagca cgattgattt catcactgcc
    13621 aggctgtggc gtgggcgtgg ttttacttac cagcacgcac cagtggctac cgctgtattc
    13681 acgcggatgc tgcttttgta cgttgaccgg gccaaacggt gcagcaacac cgacatttcg
    13741 caaatccagc gccggatcga gttcatgcat tacatggtcg ttgtaggtaa agctcaccct
    13801 ttcaccgttt gggctaaaga catgtacatg actgccgccg cgcagcgctc ctggcgtata
    13861 tggcgcggta atatccattg catcgagatt gctcaccttg ccgctttcaa caatcactcc
    13921 gcgccgatga tgaaagtcgt aataccatgt ttcatcagga ttttccgggc cgtggatgaa
    13981 gacatacttc tctgacttcg gatgaacggt caccacgccg acgtatgcgc cctgtgacgc
    14041 gcgatagata acctcgacct cgccggtatg gatattcaca cgctcaatgg tctcgccggt
    14101 aaacgacgcc ccagaaggcc gcacgtcaaa caccagccac tggctgtcgg gcgtccaggt
    14161 attggtattg gtgagcaggt gattacgggg agcaaaggtt atctgtttca tggcgcggtc
    14221 ctgatacaaa acggttcccg ccatcatact tcacaaggac ttcactttca gacaatagtc
    14281 ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat ttcgacgtgg cgattattgg
    14341 cctcggcccg gcagggtcgg cgttggcgcg aaagttagca ggcaaaatgc aggtgatcgc
    14401 gctggataaa aagcaccagt gtggtactga aggtttcagc aaaccttgtg gcggtcttct
    14461 ggcaccggac gcgcagcgat cttttattcg cgatggactg acgcttcctg tcgatgtcat
    14521 cgccaacccg cagattttca gcgtcaaaac cgtcgacgtc gccgcatcgc tcacgcgtaa
    14581 ctaccagcga agctatatca atattaatcg ccatgctttc gacttgtgga tgaaatcgct
    14641 gatccccgcc agcgttgagg tttaccacga cagcctgtgt cgaaaaatct ggcgtgagga
    14701 tgataaatgg catgtcattt ttcgcgcaga cggctgggag cagcatatta ccgcccgcta
    14761 tctggtcggt gccgatggtg ccaactcgat ggtgcggcga catctctacc cggatcatca
    14821 gattcgtaaa tatgtcgcca tccagcagtg gttcgcagag aaacatccgg taccgttcta
    14881 ctcctgcatc tttgataatg cgataactga ctgttactca tggagtatca gcaaagacgg
    14941 ttattttatc tttggcggtg cctatcccat gaaagacggt cagacgcgtt tcacgacgct
    15001 aaaagagaaa atgagcgcct ttcagttcca gtttggtaag gcggtgaaaa gcgaaaaatg
    15061 cacggtgctg tttccctcac gctggcagga ttttgtctgc ggcaaggaca acgcctttct
    15121 gattggcgaa gcggcgggat ttatcagcgc cagctcgctg gaggggatta gctatgcgct
    15181 ggatagcgca gagattctgc gttcggtatt actgaagcag ccagagaagc tcaataccgc
    15241 ttactggcgc gccactcgca aactgcgttt aaaactcttc ggcaagatag taaaaagccg
    15301 atgcctgacc gcaccggctt taagaaagtg gattatgcgc agtggagtgg cacatattcc
    15361 acagttgaaa gattatccaa cgcgcttcac atcgcccacc agcaggatgt aagagagcgc
    15421 gccaatcaac gcgacggcgg agatataaac cagtgcaggc gcgaaaccgt aaccctgcgc
    15481 caggtacccc accaccagcg gaacggtgat gccgcccaga ccaccggcaa agttaaacac
    15541 gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa gagaccagcg accaggttat
    15601 cgaagcaaaa ccgttgccga agaatgccag cgccatcagg cacataatca tcatcggatc
    15661 gttggtgtag ttcgcaccca taatgcaggt ggagatcagc aagccgcaga taatcggcgt
    15721 tttacgcgca aagcccagtg aaaagccctt acgtaccagc agatccgcga cccagccaga
    15781 gagcaggacg ccaataaacg ccgcgaggaa tggcaccgtg gtcataaaac ctgctttcag
    15841 cgccgtgatt cctttttcct gggttaaata gttcgggaac caggttaaga aaaaccacag
    15901 tgtagaagcc accgcaaatt gcccaagata gacaccgatc agtttacgat ggaacaccag
    15961 tttccagtct ttggctgtta acggctgacg cgcctctttt ttcaccggcg catcgccatc
    16021 caccagaccg ccgccatcac gaatgtaatc cagttcagct ttgctgatgc ctttggtcag
    16081 acgcggcggc tgataaacct taaaccaaat cagcgaccag ataatgccga taccaccggt
    16141 gacaatgaac acccagtgcc agctcaacat ctcctgaatc cagatcagca gcggagtcag
    16201 aaacgccaga ccgacaaact gaccagacgt ataaaaacca acggcagaag cgcgttcatg
    16261 ttccgggaac caactggtta ccatccggtt attggtcggg aacgcgggcg cttcgaaaat
    16321 accggttatc gcgcgcagac caattaatga cattaagccc gtggcaaagc cctggaataa
    16381 cgtcgccact gaccagccaa atatcgcaat aaaataagtc acgcgtgaac ctacgcgatc
    16441 taaaaaccaa ccgccgggga tctgacatag cgtataaagc caggcgaagg ccgaaaatac
    16501 atagcccatt tgcgctttag taataccaaa ctcttcctga atatgggcag aagccacggc
    16561 caggttagca cggtcaacat aacaaatgac caccgtaata aagatcatca ccagcgtcag
    16621 ataacgccga cgccccggct ttgctgcatt aacgggaata tccatagcga gctttctcct
    16681 gattttgggc atagcgaagc cgctcaccat gcactgtaat ttacagaggg ttatattttt
    16741 gtattgctgt tttagtgccc gatgaggggc ttacgtggca ggaattacca ctctgctacg
    16801 ctgttatctt catgacgcca gagcggatta cgccagtccg gggcattttt actgaactca
    16861 ataactttag cttcgtcgat ttccacgcct aagcccggtt tcgttaatgg tttaaagaaa
    16921 ccgccgacca tgctgaagtc ctctttgttt ttcacaaagt cgagtaactc cgcgcctttg
    16981 ttgtaatgaa tgcccatact ttgttcctga aggacggcgt tataggaaac aaagtcgata
    17041 tgcaggcaag ccgccagtgc aatcggtccg agcggacagt gcggcgcaag ggtcacgtca
    17101 taggcttctg ccattccggc aattttgtag cattcggtaa taccgcctgc gtgggagaga
    17161 tccggttgca gaatcgaaat accgcctgcc tccagcacac gtttaaaatc gaagcgtgag
    17221 aacatgcgct cacccgccgc cagtggaata tgcgtttgtg ccgccagttt cgggtagtat
    17281 tcggcctgtt ccgccagcac cggctcttca ataaacagcg gacgatacgg ttccagctct
    17341 ttaatcagca ctttcgccat cggcgcgctg acgcgaccat ggaagtccag accaaactca
    17401 atctgattgc caaaagcttc acgaatttgt gccacggtgt tgactgccgc atctaccgcg
    17461 cgggagttat caattagccc cagttcttca caaccgttca gtttgaaggt atcgaagccg
    17521 atttcgcgta gcgttttaat gccgtcgata acatccgccg gacgatcgcc gccgacccaa
    17581 ctgtaggctt taattttgtc gcgaaccagg ccgcccatca gttgccagac cggcgcattc
    17641 agcactttgc ctttgatatc ccataaagcc tggtcgatcc cggcgatggc actcatcaga
    17701 atcggaccgc cacgataaaa tccggcgcga tacatcactt gccataagtc gttgatgcgt
    17761 gacgggtcct gaccaatcaa atagtcactc agctcgtgga ctgccgcttc caccgtgcga
    17821 gcgcgacctt caatcacggg ctcgccccaa ccgaccacgc cttcatcggt ttcaattttc
    17881 aggaacatcc agcggggagg taaacgatac gtggtaattt tggtgatttt cattgcactg
    17941 cctctcgata cgccttaaca aatgctgctg cctgctgcgc agtccgttct acggactgcc
    18001 cggcgcgata gagatcgcta cctaagcccg ctccggcaca gcctgcatct atccactgcg
    18061 ccaggttttc tggcgtcaca ccgccaacgg caaagaccgc gatgttcggt ggtaataccg
    18121 cttttaatgc tttgatgtat tgcggaccaa aagccgatga cggaaatatt ttcagtgcct
    18181 gcgcacccgc gtcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg gggcagacgg
    18241 tcatgccgta gcccaccgca cggcggatca cttcactatg gatattgggc gtaacgatga
    18301 gctgacagcc cattctggcg agcgcatcga cctgttcagg tttcagtacc gtacctgcgc
    18361 caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg aatgctctgc tcccattgtg
    18421 gagaattcag cgggatttca accgcgtcga acccggcgtc aatcaccgcg ccaacatgcg
    18481 ccagcgcctc gtcgggcgta atgccgcgca aaatggcgat cagcgggagt ttagtttgcc
    18541 actgcatgag cgatgttcct tataccagcc tgaaatgccg tgtcgcccgc caccgccgtc
    18601 acgtcacaac ccatcgcctg aaaggcttgc tggtagcgcg cggtcagcga tgttccggcg
    18661 acaagggtga tgacttgttg atgggccaca tagtcgcgca tactggcgac ctctgcgcca
    18721 atcaacaaac cagagagaaa ttcgctgacc tgttcgcggg gaagtgttcc cagcacatgc
    18781 gaggcgcgaa cttcaaaaag ctgtggcaat acggcgggcg cattaaggcc acgctcaaga
    18841 ccagccgcga aagcatcgac agcgttttcc tgcggcggca aacctgcgcc aatcaatgag
    18901 tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc gttaatttgc
    18961 tggctatcgg cctgcaccca tttgcaatgc gtaccgggca tgacataaag agaggaagga
    19021 gcgagagtgc gcgcgccgat caattgtgtt tcttcgccac gcatcacatt gtggttatcg
    19081 tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga cgttaattgt
    19141 tcgccaatag acgaaaaacg ggcaggaatg gataaatacg gcgcaacttt ccagccgaca
    19201 ttgctgccga ccattcctgc cattaccact ggcgtattct cttcacgcca gtcggtcgtc
    19261 acttctgcta acaccgcagc cggagatttt ccattcaggc gcgtgacgcc tgcttctgat
    19321 tgcctgctgt ccaggcagtg gtcgccctga taaagccagg cgcgcagatt ggtcgatccc
    19381 cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt gtcgagctgg
    19441 cgatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgcctt atcgcatcga
    19501 acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc atccaggttc
    19561 gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct cagttgttgt aacaccgggt
    19621 tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc
    19681 ggtcctgatt gttagcaatc atctcgttca gcgccgattc aatctgcgcc agatcgctgg
    19741 aggtcgcgcg ctctgctgcc caacgggcaa tagccggctc taccaggttt cgcacttcgc
    19801 tcattgcact gataagccgt gggtcatagt cgttttccag cacccattgc agtacgtcag
    19861 tgtcgaggta attccactgg ttacgcggag ccacaaacgc cccgcgataa cgtttcattt
    19921 caatcagccg cttcgccatc agcgaacgaa atacctcacg gatgatgttg cgcgaggttg
    19981 caaactcctc acagagttct gcctcagccg gaagcggcga gcctggcacg tatttgccgt
    20041 gaacgatctg tttacccagc gtaataacga tgcgatcggt tttattgaga gtcatggaga
    20101 gtccttgtgc tctgtatgtt cctctctact ttaccccgat cgatgcataa cgcggcaatt
    20161 ttgtagtacc agcgtgatga cgttcgcgtt tgctgtgcgc ataatgtagt acaaatttat
    20221 attgttgtac tacaatttag atcacaaaaa gaacaatcca taagaaaatg ttctacgtcg
    20281 ggcaaaaatc tgaaaaggga gatcaggcgc taaacaggag ggaaagaaga gtatgctttc
    20341 aacggcttag cactcttgtt taaaggatta atcatgaaat tgaattttaa aggatttttt
    20401 aaggctgccg gtttgtgttc tttggcattt gcgttgaccg gatgtatcac ctggggattg
    20461 gtttccaata ctgcaagccg tccacatgcc gagcaatggc ggtcagacac gattaagggt
    20521 ttatcgctgg ctgaagacag taacggtacg aaaggttatg tttttgttgg tgaatcgttg
    20581 gactatctat tgaccaccgg cggcgatgag gtggttaaca tgctgaacga tccggcaatc
    20641 catggtgaaa gaatcaccgt ttctgataac gcgaaattta ttttaagcag cagtaataag
    20701 aatttctctg gtgctattac gctctattac gactggaaca acgaagaaga taaggcactg
    20761 gcaacacaat atggttttat ttgtgatacc cggcgttgta catggatgct ggacggtttg
    20821 aaaggctcaa tccaccagaa aaataaaaaa gcggattact caaacgtgat ggttttccat
    20881 caacccttta cggtgggatt ttatgagtac aaagcaacag atggcgtccc gcgcggcctc
    20941 gttaatgcgc tgctgccagt cactcttacg ctggatatag taacgtcacc actccagttc
    21001 ctgattttat gcacgaccag aaattgttaa tcgctgcggg caaacgcatt catgccgttt
    21061 gcccgttcac tgattaattc agcacatact tctcaatagc aaatgccacg ccatcttcaa
    21121 ggttagattt ggtgacaaag ttcgccactt ctttcaccga aggaatagcg ttatccatcg
    21181 ccacaccgac gcctgcatat tcaatcattg cgatgtcgtt ttcctgatcg ccaatcgcca
    21241 tgatttcttc cggtttaata cctaacacgt cggccagtga tttcacaccc gtacctttgt
    21301 taacgcgttt atcgaggatt tcgaggaagt acggcgcact tttcagcacg gtgtattttt
    21361 ctttcacttc ctgcggaata cgcgcaatgg cctggtcgag gatggcgggt tcatcaatca
    21421 tcatcacttt caggaactgg gtattggggt ccattttctc cgcttcgcag aacaccagcg
    21481 gaatggtggc aacgaaagat tcatgcaccg tgtagtagct gatatctcgg ttggcggtgt
    21541 acagcgtggt gcggtccagg gcgtggaaat gagaaccgac ttcgcgagag agtttttcca
    21601 ggaaacggta gtcgtcatag ctgagagcgg tttgcgccac ggtgctacca tcagcggcct
    21661 tctgtaccag cgcgccgtta taagtaatgc agtagtcgcc cggctgttcc atatgcagct
    21721 ctttcaggta attgtgaaca cctgcataag ggcgacccgt cgttagcacg acattcacgc
    21781 cacgggcgcg agctgcggca atcgcatttt taacggcggg tgaaatggtg tgatcgggca
    21841 gcagaagggt gccatccata tcgatagcaa tgagtttgat agccatgagt tccccaggta
    21901 gattggttcc tgacccatgc taacgcgatt ccgctcaaaa atcagtacaa cacccgaggg
    21961 aaaaggggat gcaacgcgca ttgcgtgctc cccatttgct tagcggaaga gtttcccttt
    22021 cagcagttcc atacctgcgg aaagcagatc gttattggct tgtggtgaca cttcaccttg
    22081 cggtgagagt gcatcaataa tcttcggcaa ttgttctgcc agtaaactgg aagctgtact
    22141 ggtatccacg ccaagttttt gcccgagatc ggacaccgca tttgtgccga gcgccgattc
    22201 cacttgctcg ccgctaactg attgattgct ctgttgatta ctcagccagg ttgagagaat
    22261 ggcccctaag ccgccacttt gcagtttttc cagcagcacc tgaatgccgc cctgctcctc
    22321 aacccagctc aaaatcgcct gatatttccc cgcatcgcct ttcagaaagg caccgacaac
    22381 ttcatcaaaa agccccatga taatcacctg taaagcgtta cgtgttgacc caaaaagtat
    22441 agatctgcgg gatgataatt gcggattgca ggaataaaaa gggcggagat gatctccgcc
    22501 cggactgacg actaagtcat tttatcagca acaaaaagcc attaaatatc gatattcgcc
    22561 gctttcagag cgttctcttc gataaacgca cggcgcggtt caacggcgtc gcccataagc
    22621 gtagtgaaca actggtcggc agcaatcgca tctttaacgg taacgcgcag catacgacgg
    22681 ctttccgggt ccatggtggt ttcccacagc tgttccgggt tcatctcgcc cagaccttta
    22741 taacgctgga tggagaggcc gcgacgggac tctttcacca gccagtccag cgcctgctcg
    22801 aagctggcta ccggctgacg acgttcgcca cgttcgataa atgcatcttc ttccagcaag
    22861 ccacgcagtt tctcacccag cgtgcagata cgacgatatt cgccgccagt gataaattcg
    22921 tgatccagcg gatagtcagt atccacaccg tgggtacgca cgcgaacaat cggctcgaac
    22981 aggttttgtt cggcattggt atggacatca aacttccact ggctgccgtg ctgttcttta
    23041 tcgttcagtt cgctgaccag cgcgttcacc cagcgggtaa cggtttgctc gtcagaaagg
    23101 tcggtttccg tcagcgtcgg ctgatagata agctctttca gcattgcttt cggataacga
    23161 cgctccatgc ggttgatcat tttctgcgtc gcgttgtact cagacaccag tttctctaac
    23221 gcttcgccag ccagcgccgg tgcactggcg ttggtgtgca gcgttgcgcc atccagcgcg
    23281 atagagatct ggtactgatc catcgcttcg tcgtctttaa tgtactgttc ctgcttacct
    23341 ttcttcactt tgtacagcgg cggctgagcg atgtagacgt gaccacgttc aacgatttcc
    23401 ggcatctgac gatagaagaa ggtcaacagc agcgtacgaa tgtgcgagcc gtcgacgtcc
    23461 gcatcggtca tgatgatgat gctgtgataa cgcagtttgt ccgggttgta ctcgtcacga
    23521 ccgataccac aaccaagcgc ggtgataagc gtcgccactt cctgagaaga gagcatctta
    23581 tcgaagcgcg ctttctcgac gttgaggatt ttacccttca gcggcagaat cgcctggttc
    23641 ttgcggttac gcccctgctt cgcagagccg cccgcggagt ccccttccac taggtatagt
    23701 tcggaaagcg ctgggtcgcg ttcctggcag tctgccagtt tgcccggcag gcccgctaag
    23761 tcgagcgcac ctttacggcg agtcatttca cgcgcgcgac gcgccgcttc acgggcacgg
    23821 gcagcatcga taattttgcc aaccacgatt ttcgcgtcgg ttgggttttc cagcaggtat
    23881 tccgccagca gttcgttcat ctgctgttca accgccgatt tcacctcaga agaaaccagt
    23941 ttgtctttgg tctgtgagga gaatttcggg tccggcactt tcacggaaac gaccgcaatc
    24001 aggccttcac gcgcatcgtc accggtggcg ctgactttgg cttttttgct gtagccttct
    24061 ttgtccatgt aggcgttcag agtacgggtc atcgccgcac ggaaacctgc caggtgagta
    24121 ccgccgtcac gctgcggaat gttgttggta aagcagtaga tgttttcctg gaagccatcg
    24181 ttccactgca acgccacttc gacgccaata ccgtcttttt cggtggaaaa gtagaagata
    24241 ttcgggtgga tcggcgtttt gttcttgttc agatattcaa cgaacgcctt gatgccgcct
    24301 tcatagtgga agtggtcttc tttgccgtcg cgcttatcgc gcagacgaat ggaaacgccg
    24361 gagttgagga acgacaactc acgcagacgt ttcgccagaa tttcatattc gaactcggtc
    24421 acattggtga aggtttcaag gcttggccag aaacgcacca tggtgccggt tttttcagtc
    24481 tcgccggtaa ccgccagcgg ggcctgcggt acaccgtgtt cgtagatctg acggtgaatt
    24541 ttaccctcgc gctggataac cagctccagt ttttgcgaca gggcgtttac taccgaaaca
    24601 ccaacgccgt gcagaccgcc ggacacttta taggagttat cgtcaaattt accgcctgcg
    24661 tgcagaacgg tcatgatcac ttccgccgcc gatacgccct cttccgggtg aataccggtc
    24721 ggaatgccgc gcccgtcatc ctgtacagag acagagttat cggcgtgaat ggtgacgata
    24781 atttctttac agtgacccgc gagcgcttcg tcgatagcgt tatctaccac ctcgaatacc
    24841 atgtggtgca gaccggtgcc gtcatccgtg tcgccgatat acatacccgg gcgcttacgc
    24901 accgcatcca gccctttcag gactttgata ctggaggagt cataagaatt cgacatcaac
    24961 gtttctcgct catttattct tgggttaatc cgttatttta cccttttcca cggtaaacat
    25021 cttcgaattt tcgtccgaca tgtctattac gtgttcagca ctgatcgcgc tgacaaagac
    25081 ctgtgattgc gtcgctttta agcggctggc aagcagcccg cgacgctcat catcaagctc
    25141 agaggcaaaa tcatctatca ggtagagaca ccgccgcccg ctttcacggg tgaggaactc
    25201 tccttgcgcc agacgtaagg cgcacatcaa cagcttaagc tgtccacgcg ataaggtatc
    25261 ttccaccggc gcaccgtcgg cgcgaatgcg taagtccgct ttatgcgggc cgtgcgcggt
    25321 gtaggttaac tggcgatcgc gttcaaaatt acgttccagc acctcagcgt actctgtctc
    25381 tttctcccag ccgcgctgga aagagaaagt cagagaaaac tcagggagaa attgcttaca
    25441 ggtatcagcc atatcggctg cgataccggc gctatactcc gcgcgccagg tgctgatttg
    25501 ctccgccagc gggatcagtt ctttatccca cgggcgtagc tgttcgtaac gagtcacctg
    25561 gcgcagcgcc gcattgcgct gcttgagcaa tcgcttgaga ttgctccagg cggtgaaaaa
    25621 tccgggttcg ttgtgaaagc atccccagtc gaggaatgct cttctgtatt tggggccgcc
    25681 attgagtaaa gtaaaccctt ctggcgttat cagctgcatt ggcatcaggt gcgccagctc
    25741 cgcgacttta tgcccgtctg taccgtcaat acggactttg ctgtcgcctt gtttgtcttt
    25801 ggttaaacca atcgccgtct cacgctcttc gccctgtaat cgtccgtgga gaacaaacgc
    25861 ctcctgctcg tggcgaatga cgcgaccaat ctgcaaactg cgaaacgccc gaccgtggcc
    25921 gagcgtatag atggcttcca gcacgctggt tttgccactg ccgttggcac ctaccagaaa
    25981 gttaaagccg ggagataagg cgagatccgc ggtttcaatg ttgcggaaat cgcggatcaa
    26041 caagcgggtg agggacatta cagtctcatt ggcatgacaa cataagccgc gctctggctg
    26101 gccgcatctt caatctgcac gctggaaacc gaatcggtca gcatcatgcg gacgttttcg
    26161 catttcagtg cgttcagaac atccagcaca tagctgacgt tgaagccgat ttccatctcc
    26221 gcaccgctat aggtaacgtc gaggatctct tccgcttctt cctgttctgg gttgttggcg
    26281 gtgattttca gctggttttc gctgacatac agacgcacgc cgcggaattt ctcgttagag
    26341 agaattgccg cacgggcaaa cgcctgcttg agcagatcac agccagcttc cagatgcttg
    26401 tccgggttct tcggcagaac gcgacggtaa tccgggaagc gaccatccac cagtttcgag
    26461 gtgaagataa agtcgccaac gtgggcgcga atgttgttgc tgccaatctg cacgcgcagc
    26521 ggattgtcgc cgccgtcgag catacgcatc agttcaatta cgcctttacg cggcacgatc
    26581 accgaatggc ttggcaaaga ttgaccaatt ggcattgaac agactgccag acggtggccg
    26641 tcggtcgcca cggtgcgcag ttcttcacct tcggtttcaa acagcatacc gtttaagtaa
    26701 tagcgaacgt cctgatgtgc catagaaaac tgggtcgctt caatcagacg cttcatcgtc
    26761 gcctgcggca gggtaaattc gacttcgctc tgccagtcat cgaggttcgg gaaatctgcc
    26821 gctggcaggg tagacagcga aaaacggcta cgcccggagc gcaccagcat ccgctcacct
    26881 tccagctgca cggcaatttc cgcgccttca ggcagaccac ggcagatatc aaagaatttg
    26941 cgcgccggaa cggtcgtcgc ccctggttcg tgcggctgaa ccagcgcaac gcgtgccacc
    27001 atttccatct cgagatcagt accggtcagc gacaacgtac cgtcagcaac ctgtaacagc
    27061 agattaccga gaatcggtag cgtaggacga ccacctaacg gaccgctcac ctgttgtagc
    27121 ggttttaata aatgctcacg ttctacggta aatttcatag gtttacgatg acaatgttct
    27181 gattaaatta gaaaaatctt ctttgatatc gtggctctct tcacgcaact gctcgatctt
    27241 ccggcaggca tgaagcaccg tcgtgtggtc acgaccacca aaggcatcgc caatctccgg
    27301 cagactgtgg ttagtcagct ctttcgccag cgccatcgcc atctggcgcg gacgggccac
    27361 cgagcgggat cgacgcttgg aaaggagatc cgcgactttg atcttgtagt actccgccac
    27421 cgtcttctga atattgtcga tggtgaccag tttttcctgc aatgccagca agtcgcgcag
    27481 cgcctcacgc acgaagtcga tggtgatcgc ccgtccggta aagttggcgt tggcaatgac
    27541 gcggttcagc gcgccttcca actcacgtac gttagatcgt agacgcttgg cgataaagaa
    27601 cgccacttcg cccggcaaac gaatgtcgtt ttcgtcggcc tttttcatca ggatcgccac
    27661 gcgggtttcc agctctggcg gttcgatcgc cacagtcagt ccccagccga agcgggattt
    27721 caaacgatcc tcaacgccgt tgatctcttt cggatagcga tcggaggtga gaatgatctg
    27781 ttgattacct tccagcaggg cgttgaaggt gtggaaaaac tcttcctgag atcgttcttt
    27841 attagcaaaa aactgaatat cgtcgatcag cagtgcatct acggaacggt agtagcgttt
    27901 aaactcttcg atcgcgttgt tttgcagggc tttaaccatg tcctgaacaa agcgctcgga
    27961 gtgcatataa accactttgg cattcggctt gcgcgccata atgccgttac ccaccgcatg
    28021 cagcaggtga gttttaccca aacccgtgcc gccataaagg aacaacgggt tataagcacc
    28081 gccagggtta tctgccacct ggcgagccgc cgcgcgcgcc agttggttag atttaccttc
    28141 aacgaagtta tcaaacgtgt gtttgacgtt tacgttagaa cgataggtcg gttctgccgg
    28201 agccgggacg ttatcccaac ccgagcgcgt agaaggcgca gcacgttgcg gctgcgtttg
    28261 cgccacctgt gcaggggccg cgacgttgct cgtcactgcc gcttgtggcg tttgcgtcac
    28321 cggtttggtg ccgacttcaa aacgcagctg tggggcatcc gctccgcaga aactggttag
    28381 cagtccattg atattattaa ggtacttgtc ccgtacccaa tcgaggacaa aacggtttgg
    28441 cgcgtacagg gccagcgtgt tatcgctcag ttccgcctgc aatgggcgta tccacatact
    28501 gaattctgtg gctggtaact catcctgcaa tcgggcaaga cactgctgcc aaagcgaaag
    28561 tgacacggcg gactccactc gaacaaaagt cgataatgac taaggctgaa acattcatga
    28621 ttgttgacgt acgtcgaaaa gaccctgcat gagggtgacg cacgaaccgc tgtctgcggt
    28681 tatatgcccg atcaagatcc tgcaaaacga tcgggaccgc ggatcatagc ctaaactgcg
    28741 caagagatct tctgtttctc acagattttc ccgatttatc cacaggatga tccagaactc
    28801 gctaagtgta aacgatcctg ccgcaggccg ggcacgattt acgccgcata ttggaaaatt
    28861 taatgaccat agacaaaaaa tggcttaatc gatctaataa agatccagga cgatccttgc
    28921 gctttacccg tcagcccgta taatcctcca cccggcgcgc catgctggtt ttcactggtg
    28981 tgaggccgta cgttttcccc gcgaaaaggt gcggaaaagc gcggtaaata aggaaagaga
    29041 attgactccg gagtgtacaa ttattacaat ccggcctctt taatcaccca tggcttcggt
    29101 gtccatcgtt tcatttttcg gcggatatcc aataaagcca ttgaatttat tcaagtttag
    29161 gtagaaatcg ccatgaaacg cacttttcaa ccgtctgtac tgaagcgcaa ccgttctcac
    29221 ggcttccgtg ctcgtatggc tactaaaaat ggtcgtcagg ttctggcacg tcgtcgtgct
    29281 aaaggccgcg ctcgtctgac cgtttctaag taataaagct aacccctgag tggttaagct
    29341 cgcatttccc agggagttac gcttgttaac tcccagtcaa ttcacattcg tcttccagca
    29401 gccacaacgg gctggcacgc cgcaaattac tattctcggc cgcctgaatt cgctggggca
    29461 tccccgtatc ggtcttacag tcgccaagaa aaacgttcga cgcgcccatg aacgcaatcg
    29521 gattaaacgt ctgacgcgtg aaagcttccg tctgcgccaa catgaactcc cggctatgga
    29581 tttcgtggtg gtggcgaaaa aaggggttgc cgacctcgat aaccgtgctc tctcggaagc
    29641 gttggaaaaa ttatggcgcc gccactgtcg cctggctcgc gggtcctgat agccctcatt
    29701 cgggtctatc aacgcctgat tagtccgcta ctcgggccgc attgtcgttt cactccaacc
    29761 tgttcaagct acggaattga ggcattgcgc aggtttggag tgataaaagg cagttggttg
    29821 acggtgaaac gcgtattaaa atgccaccct ttacaccctg gtggtgacga tcccgtcccg
    29881 cccggaccat ttgataccag agaacactaa cgatggattc gcaacgcaat cttttagtca
    29941 tcgctttgct gttcgtgtct ttcatgatct ggcaagcctg ggagcaggat aaaaacccgc
    30001 aacctcaggc ccaacagacc acgcagacaa cgaccaccgc agcgggtagc gccgccgacc
    30061 agggcgtacc ggccagtggc caggggaaac tgatctcggt taagaccgac gtgcttgatc
    30121 tgaccatcaa cacccgtggt ggtgatgttg agcaggccct gctgccggct tacccgaaag
    30181 agctgaactc tacccagccg ttccagctgc tggaaacttc accgcagttt atttatcagg
    30241 cacagagcgg tctgaccggt cgtgatggcc cggataaccc ggctaacggc ccgcgtccgc
    30301 tatataacgt tgaaaaagac gcttatgtgc tggctgaagg tcaaaacgaa ctgcaggtgc
    30361 cgatgacgta taccgacgcg gcaggcaaca cgtttaccaa aacgtttgtc ctgaaacgtg
    30421 gtgattacgc tgtcaacgtc aactacaacg tgcagaacgc tggcgagaaa ccgctggaaa
    30481 tctccacctt tggtcagttg aagcaatcca tcactctgcc accgcatctc gataccggaa
    30541 gcagcaactt cgcactgcac accttccgtg gtgcggcgta ctccacgcct gacgagaagt
    30601 acgagaaata caaattcgat accattgccg ataacgaaaa cctgaacatc tcttcgaaag
    30661 gtggttgggt ggcaatgctg caacagtatt tcgcgacggc gtggatcccg cataacgacg
    30721 gtaccaacaa cttctacacc gctaatctgg gtaacggcat cgccgctatc ggctataaat
    30781 ctcagccggt actggttcag cctggtcaga ctggcgcgat gaacagcacc ctgtgggttg
    30841 gcccggaaat tcaggacaaa atggcagctg ttgctccgca cctggatctg accgttgatt
    30901 acggttggtt gtggttcatc tctcagccac tgttcaaact gctgaaatgg atccatagct
    30961 ttgtgggtaa ctggggcttc tccattatca tcatcacctt tatcgttcgt ggcatcatgt
    31021 acccgctgac caaagcgcag tacacctcca tggcgaagat gcgtatgctg cagccgaaga
    31081 ttcaggcaat gcgtgagcgt ctgggcgatg acaaacagcg cataagccag gaaatgatgg
    31141 cgctgtacaa agctgagaag gttaacccgc tgggcggctg cttcccgctg ctgatccaga
    31201 tgccaatctt cctggcgttg tactacatgc tgatgggttc cgttgaacta cgtcaggcac
    31261 cgtttgcact gtggatccac gacctgtctg cacaggaccc gtactacatc ctgccgatcc
    31321 tgatgggcgt aacgatgttc ttcattcaga agatgtcgcc gaccactgtg accgacccga
    31381 tgcagcagaa gatcatgacc tttatgccgg tcatcttcac tgtgttcttc ctgtggttcc
    31441 cgtcaggtct ggtgctgtac tatatcgtca gcaacctggt aaccattatt cagcagcagc
    31501 tgatttaccg tggtctggaa aaacgtggct tgcatagccg cgagaagaaa aaatcctgat
    31561 tcggtgagtt ttcgctaaaa taagggcggt cagttgaccg ccttttttct tttcgtaggg
    31621 cggataagca ccacacatcc gccacacaaa gcaacaggaa catcatgagc gataatgaca
    31681 ctatcgtagc ccaggccacg cctccgggac gtggcggcgt tggcatcctg cgcatctccg
    31741 gcctcaaagc ccgtgaagtt gccgaaaccg tgctgggtaa actgcctaag ccacgctacg
    31801 ccgattatct tccgtttaaa gacgccgatg gcagcgtgct cgatcagggg attgcgctat
    31861 ggttccctgg cccgaactcg ttcaccggcg aagatgtgct ggaactgcaa ggtcatggcg
    31921 gtccggtgat cctcgacctg ctgttaaaac gcattctgac cattcccggc ctgcggattg
    31981 cccgccctgg tgagttttcc gaacgcgcat ttctgaacga taaactcgac ttagcccagg
    32041 ccgaggcgat tgcggatctt atcgacgcca gctcggaaca ggcggcccgt tcggcgctaa
    32101 actcgctgca aggcgcgttc tccgcacggg tcaaccatct ggtggaagcc ctcacccact
    32161 tgcgcattta cgtcgaagcg gcaattgatt tcccggatga agagatcgat ttcctctccg
    32221 acggcaaaat tgaagcccag ctcaatgacg taattgccga tctcgacgcg gtacgcgccg
    32281 aagctcgtca gggtagtttg ttgcgcgaag ggatgaaagt ggtgattgcc ggacgtccta
    32341 acgccggtaa atcgagcctg ctgaacgcgc tggcgggccg tgaagcagca atcgtaaccg
    32401 atatcgccgg aaccacgcgt gacgtgctgc gtgagcatat ccacattgac ggaatgccgt
    32461 tgcatatcat cgataccgcc gggctacgtg aagccagtga cgaagttgaa cgtattggta
    32521 tcgagcgcgc atggcaggaa attgaacagg cagaccgcgt gctgtttatg gtcgatggca
    32581 ccacgacaga cgccgttgat ccggcagaga tctggccgga atttatcgcc cgtctgccag
    32641 cgaaactgcc gatcaccgtg gtgcgcaata aagccgatat caccggcgaa acgttgggga
    32701 tgagcgaagt gaacggtcac gcgttaattc gtctttcggc gcggactggc gaaggcgtgg
    32761 atgtgctgcg taaccatctc aaacagagca tgggctttga cactaatatg gaaggcggct
    32821 tcctggcgcg tcgtcgtcac ctacaggcgc tggaacaggc ggcagagcat ttgcagcaag
    32881 gtaaagcgca actgttaggt gcctgggcgg gtgaactgct ggcggaagag ttgcgcctgg
    32941 cgcagcagaa cttaagcgag atcaccgggg agtttacttc agacgaccta ctggggcgga
    33001 ttttctccag cttctgtatc ggtaagtaac cgcgcttgcg aagccgcatt ctgactgtca
    33061 gatgcggctt cgcttcattg ttagcactcc tgttattcct caaccctt
//
LOCUS       DACADF010000040        33049 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.040, whole genome shotgun sequence.
ACCESSION   DACADF010000040 DACADF010000000
VERSION     DACADF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.040"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            394..1461
                     /locus_tag="ISB30_001796"
     CDS             394..1461
                     /locus_tag="ISB30_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP1989434.1"
                     /translation="MDKSKRHLAWWGVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1458..4193
                     /gene="rbbA"
                     /locus_tag="ISB30_001797"
     CDS             1458..4193
                     /gene="rbbA"
                     /locus_tag="ISB30_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAP1989435.1"
                     /translation="MMHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGGVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4193..5317
                     /locus_tag="ISB30_001798"
     CDS             4193..5317
                     /locus_tag="ISB30_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAP1989436.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLIAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(5426..6277)
                     /locus_tag="ISB30_001799"
     CDS             complement(5426..6277)
                     /locus_tag="ISB30_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HAP1989437.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVSPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRSVK"
     gene            complement(6309..6578)
                     /locus_tag="ISB30_001800"
     CDS             complement(6309..6578)
                     /locus_tag="ISB30_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAP1989438.1"
                     /translation="MLTKTVEVRNSTGLHARPAACLAKAAKKCSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTALFRDRFGEAE"
     gene            complement(6568..8076)
                     /locus_tag="ISB30_001801"
     CDS             complement(6568..8076)
                     /locus_tag="ISB30_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAP1989439.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKATALLHVPCSAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLNRGYADYLA
                     GNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLISSVSLGLTMEFANC
                     FACFNVPGTSVLKVIGPATHNPLWLQLKADILQRPVEAIAFSEAVSVGALLTAAPDIP
                     PPQVTIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVD"
     gene            complement(8069..9427)
                     /locus_tag="ISB30_001802"
     CDS             complement(8069..9427)
                     /locus_tag="ISB30_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP1989440.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALFFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSSGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGIFLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIALVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(9504..9785)
                     /locus_tag="ISB30_001803"
     CDS             complement(9504..9785)
                     /locus_tag="ISB30_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP1989441.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(9782..10255)
                     /locus_tag="ISB30_001804"
     CDS             complement(9782..10255)
                     /locus_tag="ISB30_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP1989442.1"
                     /translation="MQGIQFQENFIQRLPAGLSVEQIIRQLAQPLVTAELVVPDFADH
                     VLEREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVVFADMGGDPDPVPVR
                     VIFLLALGESNKQLNALGWIMEMIQDTPFMCALLTMETTEIHTAILNKMKERGEI"
     gene            complement(10280..11026)
                     /locus_tag="ISB30_001805"
     CDS             complement(10280..11026)
                     /locus_tag="ISB30_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP1989443.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIV
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPVEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(11225..11626)
                     /gene="nikR"
                     /locus_tag="ISB30_001806"
     CDS             complement(11225..11626)
                     /gene="nikR"
                     /locus_tag="ISB30_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAP1989444.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(11632..12438)
                     /gene="nikE"
                     /locus_tag="ISB30_001807"
     CDS             complement(11632..12438)
                     /gene="nikE"
                     /locus_tag="ISB30_001807"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAP1989445.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARTSEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(12435..13199)
                     /gene="nikD"
                     /locus_tag="ISB30_001808"
     CDS             complement(12435..13199)
                     /gene="nikD"
                     /locus_tag="ISB30_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAP1989446.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(13199..14032)
                     /gene="nikC"
                     /locus_tag="ISB30_001809"
     CDS             complement(13199..14032)
                     /gene="nikC"
                     /locus_tag="ISB30_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAP1989447.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(14029..14973)
                     /gene="nikB"
                     /locus_tag="ISB30_001810"
     CDS             complement(14029..14973)
                     /gene="nikB"
                     /locus_tag="ISB30_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAP1989448.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(14973..16547)
                     /gene="nikA"
                     /locus_tag="ISB30_001811"
     CDS             complement(14973..16547)
                     /gene="nikA"
                     /locus_tag="ISB30_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAP1989449.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(16658..17245)
                     /gene="acpT"
                     /locus_tag="ISB30_001812"
     CDS             complement(16658..17245)
                     /gene="acpT"
                     /locus_tag="ISB30_001812"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAP1989450.1"
                     /translation="MYRIVLGKVSTLSAAPLPLGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(17300..18349)
                     /locus_tag="ISB30_001813"
     CDS             complement(17300..18349)
                     /locus_tag="ISB30_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP1989451.1"
                     /translation="METPQPDKTGMRILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            18448..19698
                     /locus_tag="ISB30_001814"
     CDS             18448..19698
                     /locus_tag="ISB30_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1989452.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFMSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            complement(19702..20259)
                     /gene="dcrB"
                     /locus_tag="ISB30_001815"
     CDS             complement(19702..20259)
                     /gene="dcrB"
                     /locus_tag="ISB30_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAP1989453.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(20332..20997)
                     /locus_tag="ISB30_001816"
     CDS             complement(20332..20997)
                     /locus_tag="ISB30_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAP1989454.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            21207..21452
                     /gene="tusA"
                     /locus_tag="ISB30_001817"
     CDS             21207..21452
                     /gene="tusA"
                     /locus_tag="ISB30_001817"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAP1989455.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(21554..23752)
                     /gene="zntA"
                     /locus_tag="ISB30_001818"
     CDS             complement(21554..23752)
                     /gene="zntA"
                     /locus_tag="ISB30_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAP1989456.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAATGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLSNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(23826..24452)
                     /locus_tag="ISB30_001819"
     CDS             complement(23826..24452)
                     /locus_tag="ISB30_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAP1989457.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            24593..24952
                     /locus_tag="ISB30_001820"
     CDS             24593..24952
                     /locus_tag="ISB30_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAP1989458.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(24955..25224)
                     /locus_tag="ISB30_001821"
     CDS             complement(24955..25224)
                     /locus_tag="ISB30_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAP1989459.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(25214..25810)
                     /gene="rsmD"
                     /locus_tag="ISB30_001822"
     CDS             complement(25214..25810)
                     /gene="rsmD"
                     /locus_tag="ISB30_001822"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAP1989460.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     EKGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            25960..27456
                     /gene="ftsY"
                     /locus_tag="ISB30_001823"
     CDS             25960..27456
                     /gene="ftsY"
                     /locus_tag="ISB30_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP1989461.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQEQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
                     AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGLRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            27459..28127
                     /gene="ftsE"
                     /locus_tag="ISB30_001824"
     CDS             27459..28127
                     /gene="ftsE"
                     /locus_tag="ISB30_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP1989462.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            28120..29178
                     /gene="ftsX"
                     /locus_tag="ISB30_001825"
     CDS             28120..29178
                     /gene="ftsX"
                     /locus_tag="ISB30_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAP1989463.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYASHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            29423..30277
                     /gene="rpoH"
                     /locus_tag="ISB30_001826"
     CDS             29423..30277
                     /gene="rpoH"
                     /locus_tag="ISB30_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAP1989464.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            30548..31651
                     /locus_tag="ISB30_001827"
     CDS             30548..31651
                     /locus_tag="ISB30_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP1989465.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLSWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            31800..32066
                     /locus_tag="ISB30_001828"
     CDS             31800..32066
                     /locus_tag="ISB30_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP1989466.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            32073..32600
                     /locus_tag="ISB30_001829"
     CDS             32073..32600
                     /locus_tag="ISB30_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP1989467.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTPDD"
     gene            complement(32597..32980)
                     /gene="panM"
                     /locus_tag="ISB30_001830"
     CDS             complement(32597..32980)
                     /gene="panM"
                     /locus_tag="ISB30_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAP1989468.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKR"
BASE COUNT         7884 a         8607 c         9002 g         7556 t
ORIGIN      
        1 ctatgtgata gtcatcaggt ttccctgtta cggaaacctg tgcaacagaa tctgtgaacc
       61 tgtacagaat atttttggaa aaaacaccag cgaacggaag aacatcgcga tgtatcacaa
      121 gttgatggca atcaaataga caacatgaat ataactgtta gtatttaatt gatttcactt
      181 atgagtatgc gtgtttcatt tacggccagg aatgctgatg atgacaacat tactacccgt
      241 tttcactaag ccttccccat tggcgctcaa tgctctgtgc gctggtcgta tttgccattt
      301 ccttcttatc ccggatggaa gaatccgctg aacgtcgttc ggtggcagtt gtaacacgtg
      361 tcgattgctt acagggatta tggcgggtcg attatggata agagtaagcg ccatctggcg
      421 tggtggggtg tcggggcact ggcggtggct gccgtcgtgg cgtggtggct gttgcgcccg
      481 gcgggtgtgc cggaaggctt tgctgtcagc aatgggcgca ttgaagcgac ggaagtggat
      541 atcgccagca aaattgccgg gcgtatcgac accattctgg tgaaagaagg ccagtttgtt
      601 cgagaaggtg aagtgctggc gaagatggat actcgcgtgt tgcaggaaca gcgactggaa
      661 gccatcgcgc aaatcaaaga agcacaaagt gccgttgccg ccgcgcaggc tttgctggag
      721 caacgccaaa gcgaaactcg tgccgcacag tcgctggtta atcaacgcca ggcagaactg
      781 gactccgtag caaaacgtca tacgcgttcc cgctcactgg cacaacgggg ggcaatttct
      841 gcgcaacagt tggatgacga ccgcgccgcc gctgagagcg cccgagctgc gctggaatcg
      901 gcaaaagctc aggtttctgc ttctaaagcc gctatagagg cggcgcgcac caatatcatt
      961 caggcgcaaa ctcgcgtcga agcggcacaa gccactgaac ggcgcattgc cgcagatatc
     1021 gatgacagcg aactgaaagc cccgcgtgac ggacgcgtgc agtatcgggt tgccgagcca
     1081 ggcgaagtgc tggcagcagg cggtcgggtg ctgaatatgg tcgatctcag cgacgtctat
     1141 atgaccttct ttctgccaac cgaacaggcg ggcacgctga aactgggcgg tgaagcacgt
     1201 ttgattcttg atgctgcacc agatctgcgt attcccgcga ccatcagttt tgtcgccagc
     1261 gtcgcccagt tcacgccaaa aactgtcgaa accagcgatg agcggctgaa attgatgttc
     1321 cgtgtcaaag cgcgtatccc accggaatta ctccagcagc atctggaata tgtcaaaacc
     1381 ggattgccgg gcgtagcgtg ggtgcgggtg aatgaagaac ttccgtggcc tgacgacctg
     1441 gtggtgaggt tgccgcaatg atgcatctgg aactggttcc cgtcccgcct gtcgcgcaac
     1501 tggcgggcgt gagccagcat tatggaaaaa ccgttgcgct gaacaatatc acactcgata
     1561 ttccggcccg ttgcatggtc gggctgattg gaccggatgg tgtcggtaaa tcgagcttgt
     1621 tgtcgttgat ttccggtgct cgcgtcattg agcagggcgg cgtgatggtg ctgggcggcg
     1681 atatgcgcga cccgaagcat cgccgcgacg tctgcccgcg catcgcctgg atgccgcagg
     1741 ggctgggcaa aaatctctac cacaccttgt cggtgtatga aaacgtcgat tttttcgccc
     1801 gcctgttcgg tcacgacaaa gcggagcggg aagtgcgaat taatgagctg ctgaccagca
     1861 ccggattagc accgtttcgc gatcgtccgg cagggaaact ctccggcggg atgaagcaaa
     1921 aacttgggct gtgctgtgcg ttaatccacg acccggaatt gttgattctt gatgagccaa
     1981 caacgggggt tgacccgctc tctcgcgccc agttctggga tctgatcgac agtattcgcc
     2041 agcggcagag caatatgagc gtgctggttg ccaccgccta catggaagag gccgaacgct
     2101 tcgactggct ggtagcgatg aatgccggag aagtgctggc aaccggtagc gccgaagagt
     2161 tgcggcagca aacgcaaagc gccacgctgg aagaagcatt tataaatctg ttaccgcaag
     2221 cacaacgcca ggcgcatcag gcggtagtga tcccaccgta tcaacctgaa aacgcagaga
     2281 ttgccatcga agcgcgcgat ctgaccatgc gttttggttc cttcgttgcc gttgatcacg
     2341 tcaatttccg cattccacgc ggggagattt ttggttttct tggttcgaat ggctgcggta
     2401 aatccaccac catgaaaatg cttaccggat tgctgcccgc cagcgaaggt gaggcgtggc
     2461 tgttcgggca gccggttgat ccaaaagata tcgatacccg ccgtcgtgtg ggctatatgt
     2521 cgcaggcgtt ttcgctctat aacgaactca ccgtgcggca aaacctggag ttacatgctc
     2581 gtctgtttca tatcccggaa gcggaaattc ccgcgcgagt ggctgaaatg agcgagcgtt
     2641 ttaagctcaa cgacgtcgaa gatgtattgc cggagtcatt gccgctcggc attcgccagc
     2701 ggctatcgct ggcggtggcg gtgattcatc gcccggagat gctaatcctc gatgagccga
     2761 cttctggcgt cgatccggtg gcgagggata tgttctggca gttgatggtg gatctctcgc
     2821 gccaggacaa agtgactatc ttcatctcca cccactttat gaacgaagcg gaacgttgtg
     2881 accgtatctc actgatgcac gccggaaaag tgctcgccag cggtacaccg caggagttgg
     2941 ttgagaaacg cggagccgcc agtctcgaag aggcatttat cgcttatttg caggaagcgg
     3001 cagggcagag caacgaagcc gaagcgccgc ctgtggtaca tgacactacc cacgcgccac
     3061 gtcagggatt cagcctgcgc cgtctgttta gctacagccg ccgcgaagcg ctggaactgc
     3121 gacgcgatcc ggtacgttcg acgctggcgc tgatgggaac ggtgatcctg atgctgataa
     3181 tgggttacgg catcagtatg gatgtggaaa acctgcgctt tgcggtgctt gaccgcgatc
     3241 aaaccgtcag tagccaggcg tggacgctca atctctccgg ttcccgttac tttatcgaac
     3301 agccaccgct caccagttat gacgagcttg atcgtcggat gcgtgcgggc gatatcacgg
     3361 tggcaattga gatccctccc aatttcgggc gcgatatcgc gcgtggtacg cccgtggaac
     3421 tcggtgtctg gatcgacggt gcgatgccga gccgcgccga aacggtaaaa ggctacgtgc
     3481 aggccatgca ccagagctgg ctacaggatg tggcgagccg acagtccaca cccgccagcc
     3541 aaagcgggct gatgaatatt gagacgcgct atcgctataa cccggacgta aaaagcctgc
     3601 cggcgattgt tccggcggtg atcccgcttc tgctgatgat gatcccgtca atgctaagcg
     3661 cccttagcgt ggtgcgggaa aaagagctag ggtcgattat caacctttac gtgaccccca
     3721 ccacccgtag cgaattttta cttggcaaac agctgccata catcgcgctg gggatgctga
     3781 actttttcct gctctgcgcc ctgtcggtgt ttgtgtttgg cgtaccgcat aaaggcagtt
     3841 tcctgacgct caccctggcg gcgctgctgt atatcatcat cgccaccgga atggggctgc
     3901 tgatctccac ctttatgaaa agccagattg ccgccatttt cggtacggca attatcacgt
     3961 tgatcccggc gacacagttt tccgggatga tcgatccggt agcttcgctg gaaggtccag
     4021 gacgttggat cggcgaggtt tacccgacca gtcattttct gactatcgcc cgcggaacgt
     4081 tctcgaaagc gctggatctg actgatttgt ggcaactttt tatcccgttg ctgatagcca
     4141 taccgctggt gatgggctta agcatcctgc tgctgaaaaa acaggaggga tgatgcgcca
     4201 tttacgtaat atttttaatc tgggtatcaa agagttgcgc agtctgctcg gtgataaagc
     4261 gatgctgacg ctgattgtct tctcgtttac ggtgtcggtg tattcgtcag cgaccgttac
     4321 gccaggatcg ttgaacctcg cgccgatcgc cattgccgat atggatcaat cgcagttatc
     4381 gaaccggatc gttaacagct tctatcgccc gtggtttttg ccaccggaga tgatcaccgc
     4441 cgatgagatg gatgccgggc tggacgccgg acgttatacc ttcgcgataa atattccgcc
     4501 taattttcag cgtgatgtcc tcgccggacg ccagccggat attcaggtga acgttgatgc
     4561 cacgcgcatg agccaggcat ttaccggcaa tgggtatatc cagaatatta tcaacggtga
     4621 agtgaacagc tttgtcgcgc gctaccgtga taacagcgaa ccgttggtat cgctggaaac
     4681 acggatgcgc tttaacccga accttgatcc cgcgtggttt ggcggggtga tggcgatcat
     4741 caacaacatt accatgctgg cgattgtatt gaccggatcg gcgctgatcc gcgagcgtga
     4801 acacggcacg gtggaacact tactggtgat gccgataacg ccgtttgaga tcatgatggc
     4861 aaaggtctgg tcgatggggc tggtggtgct ggtggtgtcg ggattatcgc tggtgctgat
     4921 ggtgaaaggc gtgctgggcg taccgataga aggctcgatc ccgctgttta tgctgggcgt
     4981 ggcgctcagt ctgtttgcca ccacgtcaat cggcattttt atggggacga tagcgcgttc
     5041 aatgccgcaa ctggggctgc tggtgattct ggtgctgctg ccgctgcaaa tgctttccgg
     5101 tggttccacg ccgcgcgaaa gtatgccgca gatggtgcag gacattatgc tgaccatgcc
     5161 gacgacacac tttgtcagcc tcgcgcaggc catcctctat cgtggtgccg gattcgaaat
     5221 cgtctggccg cagttcctga cgctgatagc gattggcggc gcgtttttta ccattgcgct
     5281 gctgcgattc aggaagacga ttgggacaat ggcgtaaata cagtgcccga tacgcaagct
     5341 tatcgggcct acgcgatggt gcaatttatt gaattctagt ggttttgtag gccggataag
     5401 gcgtcacgcc gcatccggcg aggatttatt ttaccgaccg atgcccctca ttcatcatca
     5461 ggattttgct atacacatct tcctcgacgg cgttttttgc tgatgccatc actcgtgcca
     5521 gattcgcctc gttgtgatta ttcacatacg ctttgccgac tgcggtgata aacgctttgc
     5581 gcagatcgct ggcgatattc actttagcga cgcgataatt aacgaaactg cgtaatatct
     5641 ccggggcgat accggagccg ccgtggataa ccagcggcac cgggctgact tcggcaatgc
     5701 gcttcagcag ggggatatcg atccgcggta tatcgtccag gccatgaacg ttaccaatag
     5761 aaactgccag catgtcgcaa ccggtgcgtt cgacaaaggt cttcaccttt tccggttccg
     5821 ttttgcaatc ggcctcgctg acgtgatcat cttctttgcc gagaatcgcc cccagctccg
     5881 cttcaaccgg cacgccataa gatttacaga aatcgacagc ttcctgggtg aaagcgatat
     5941 tctcttcaaa agagaatgcg gcaccgtcaa tcatgaccga ggtaaagccc gcgcgcaccg
     6001 cctgtttaac atcctcctgg gttttcccgt gatccagatg aagactgacc ggaacatcca
     6061 ttttatcggc gtgacgtttc acgatttcat atatgtaatc gtatcccgac agttgtgcat
     6121 tggtgggcgc aatctgaatg aagttgggta atcccgaacg ctcaatagcg tttaaaattg
     6181 aaattgtcgt ctccagattg gtggtattaa acgcgcccgc taatacccgt ttttcctgaa
     6241 tgcggtcgag cagtatccgt ccattcacta atggcatatt gaaaacctca ttttttaata
     6301 tcagttaatt attcagcttc accgaagcgg tcgcgaaata aggcggtgag agtctgtatg
     6361 gcctcgtttt catcatcacc gtcgcagcgt atttccaccg tttttccgcc ttttattccg
     6421 gcgcgcataa tattcatcat actggtggca ttaatatcat taccctcata gtgcagcgtc
     6481 actttacagc tacatttttt ggcggctttc gccagacagg ccgccggacg tgcatgcagg
     6541 ccagtactgt ttctgacctc cacggtttta gtcaacatag tgctcctccc gatgatgtaa
     6601 tggcatcacg ccttcttgct gtaatttcaa ctgataccag cttttccatt tgtgctgata
     6661 acgctgtaat tgatgatatc ttgcccgatt cggtaacaaa cgttgggcta tggtaacttg
     6721 cggcggcgga atatccggtg ccgcggttaa taaggctccg acagaaaccg cctcgctaaa
     6781 tgcaattgct tcaaccggac gctgtaaaat atctgccttt aattgcagcc aaagggggtt
     6841 atgggtcgcc ggaccgatca cttttaacac gctggtgcca ggcacattaa aacaggcaaa
     6901 gcagttggca aattccatgg ttaatcccag tgagacgctg gaaattaaca tgtcgatatt
     6961 taacgtatcg ccaagcccat aaaataatcc gcgggtgtgg cgatttttat agggtgaacc
     7021 cgaaccgcga agatgcggaa taaagacggg aatgtcatca agcgaccagt tccccgccag
     7081 gtaatccgca tacccacgat tgagcgacgc ggcgatctcc tcatcagtta gccggaacgt
     7141 gttgcgcagc cactccagcg cgaaaccgcc cacgggcaga gaagcaaaca gggtgaactg
     7201 gttggcaagc gagtaacagc cgtttgccag cttgtcacgt ttggcctgtt catccagcgt
     7261 cggttgtgta tccagttgca gaatgccttc cgtggtgccc gtcgagttaa ggatatcgcc
     7321 cggcatcatc tgcaacgccc gcgcacccac catatggtca tgtccggcca gcgtcacgct
     7381 gaccggttgc gagaacccaa gcgttttgca gagcgactcg ctgacccatc cggcgtgttc
     7441 gcctggctgc accaatggcg caaatgctga acagggaacg tgtaacaacg ccgtcgcttt
     7501 cgctgaccat tcgcctttgc gcacgtccag acacagtgta cggctggcta aggtgatatc
     7561 cgttcgctgt tcgccagtca gccgccagag cagcacttcc ggtgcatgta gccagcgcag
     7621 gccgcgacga ttgcgcagcg gataatgctc cagcaaccaa cgcattttga aggcagagta
     7681 attgctgtgc agtggtaatc cgcaaatgtc ataaagtgcc gcactgtctg cctcgctaag
     7741 cgttgccaga tactcttcac cgcgacggtc ataccatgcc agcattggcg tcaggatctc
     7801 gccatgttcg tcaagaaaca cccctgactc gccaaaactg gcaatgctga tccgcctgac
     7861 aggcagcggt gaagcggcgt tcagttgcgc tatcgcctgg cggacctcct gccagagggc
     7921 gtcgatatcg aaatcgacat cgccctgtgg ggagatctgt tttgccgtca cgaatttatg
     7981 cgcgcccaac gtcgttgcgt ccaggcagga aaagcaggtg actttgcaat tggtggtgcc
     8041 aatatcgata acgatagcgg cgctgttatc aggcataaac cgtgctccgc gtcattcgga
     8101 aaatgccata cagcaccagg gcaataaccg gcaccagagc aatggcgatg agattgccgt
     8161 tgaagatttc cagcagccag aagcggaacg ggttaccgcc atcaaggaaa ctggaaacct
     8221 ggccgctgga gccttcggca aatttgaagt taacgtcttt cgccattccg gtaataaacg
     8281 gcgcgatttt ggtagcaatc cacaggtcgg caataatcac cgggatcgcc gccagaacgg
     8341 tgcggaaaat gttgccacgg ctggctaaag caatcatcga cgccattacc gccaggttgg
     8401 caaggtcgcc aagcggcagg acttcattac ctgggaggac aaacgcgata aagaccgaga
     8461 ttggcgttag cagcagggcg gtggagataa tcgccggatt acctacggca acggcgatat
     8521 ccaggccgat atagagatcg tcacggtcag ggtatttggc attgagatac tttttaatgg
     8581 cttcagacag cggtaataaa ccttccatca gaatgcgaac catgcgtggc aggatgaaca
     8641 tcacaccgcc aatgctgatg ccaagcagca atactttttt gaaatcgtat ccggcaatta
     8701 cgccgagaaa aatgcccaga atagtgccaa ccatcatcgg ttcgccgaaa atgccaaact
     8761 ttttctgtac ggtttccgga tcgatatgaa tacgattgag gccggggata tggtcgatga
     8821 ttttgtcgac caacagaccg accggaaaga acaccaccga agagagcgtc ggcaaagaga
     8881 tcccttccag gccaaagtat ttttgtacca gtggcgcaga ccagtcggcg agtttaagta
     8941 ctaccaccgc agcaatcgcc gccgccagca aaccaaagaa caggctgccg gagctgtaat
     9001 aaaccagcgt accggcaagg gcaaaatgcc agtagttcca catatctacg tcaacggtgc
     9061 gggttttatt taacgccagc atcgccacgt taatcagaat agtcagggga ataacaaacg
     9121 gggcaattgg cgaaccccat gtaatagcgg aaagcggcgg ccagcctaaa tccaccaccg
     9181 gtaaattaat tccggtgcgt tcaaccatcg cctgggccgc tggcccgaca ttgctggtaa
     9241 gaatatcgaa aatggcataa ataccgacaa aaccaatgcc gaccgttaat gacgaacgta
     9301 aggctttagc gggttttact ttgaaaaaga gagcaagaat aaataacacc aacggcaaca
     9361 ttaccgtggg accaaatccc aatatatatt gcacaatatt atagagggta tgcgcgatat
     9421 cgttcataat aaacctcttt ttcaggcgag agcgcacctc gcgttagcgc ctgatgtagt
     9481 gtgttgaaat taattattat taattacttt tttaggatag aaacaatttg cgccaatact
     9541 ttctcttcgc caataccagt aataattggc aagccgttaa ccaccggcac gtcgagttca
     9601 taaggcactt tagtcgttga aacaaccagt gctacgccgt cggtatttcc aggtaaagag
     9661 gcaacgttgg tttgagaata attaacgtta agaccgtgct ctttacaata ttccatgact
     9721 ttttctgtta ctgccgtgga tgtggcaata cccgtggcgc agacaaataa aatggtttga
     9781 ctcatatttc acctcgttct ttcattttgt ttaagattgc tgtgtgtatt tctgttgttt
     9841 ccatcgtaag cagggcgcac ataaagggcg tatcctgaat catctccata atccacccca
     9901 gggcatttaa ttgtttgtta ctttcgccta aggcgagcaa aaaaatcacc ctgacaggta
     9961 cgggatcagg gtcgccgccc atatcggcaa acactaccgg ttccggcagg atgccgacgg
    10021 caattgcatt gtgcctgaca tgtttatggt ccgtgtgcgg aatggcgaca cacggtggtt
    10081 cggtaggcag tcccgttggg tacgtcgcct cacgttccag tacatgatcg gcaaaatcag
    10141 ggaccaccag ttcggcggta accaacggtt gcgctaactg acggatgatc tgttcaacgc
    10201 ttaaccctgc cggaagtcgt tgaataaaat tttcctgaaa ctgtatgcct tgcattttat
    10261 tcctcttaaa aaagtgtact taactcactt cccgccgttg cagcacggtg ccaagatagt
    10321 atttatttcc ttttaaccag acgttgccaa actcaacggg taatccgcgg gagaaaaaga
    10381 ctaactgctc cagatgcaaa acgggtgcat cttcactgat atctaaaaaa tgaccgcgtt
    10441 cattaccaat taatcgcgcg gcatagcgac tttccgagta acgaattttt ctttgcgaca
    10501 aactttctat tgttggaaat aaattgtgtt gattaaaatc gatttccacg atgccggggc
    10561 atagctcaat attgatacgg ttctctatca gcatcgcttt ttcatcacca attgaacgta
    10621 aacgctcaag gtaaagaata tcctgaccgg gcgttattct taatttctct gccacataac
    10681 gattagccgg ttcaatccgt gacgtaataa cttcagtggt aaaatgtatt ttctggcttt
    10741 ccagcgattc cgcgaatgac aataatcctt cacctaacgg atatgccacg ttctcttttt
    10801 tgacaaatgt tcccttcccc tgaacctgaa tcaacacgcc ttcttttacc agttgggcga
    10861 ccgctttttt aacggttccc cgactcactt ccagcatatc cataatctgg tattccgaag
    10921 gaatgcgatc gtcttccacc agatccccgg tataaatact ttctcttatc cactccacca
    10981 cttgttgata gagcggtatt tgtgacgact tactcaatga tttcatggcg cgcacctgaa
    11041 catttacagt tgtctatgga caatcatgga caacttcaag cgtatagtag gtgaacaggc
    11101 gaaaaagagg atcgaagcgt ctcacatttt aggggaccaa atcgtgatca aaaaaatgtg
    11161 acgtgagtcg atatttggca ggagtctact gcgccgtcag gaaataacgg cgcgttggga
    11221 ggtgttaatc ttccttcggc aggcactgca aatgcccgtg ccgcacgccg cgctgggcga
    11281 tgacgtcgtc ggcaaaatgt tgtacgtcac ccatgtcacc tttcaacacg gcgatttcca
    11341 gacagtcgtc gtgattaatg tgcacatgca gagtagcaac cgagagatcg tggtgatgat
    11401 gctgggtgga gacaatacgg ctggctaagt cacgtttttc gtgttcatac acatacgaca
    11461 gcaccgcgaa accttgtgtg ccgtgttgtt gagtggcttc ctgcgccaga gcgctacgca
    11521 gaatatcgcg gatcgcctcg gaacggttgt tataaccacg acgctggctc aggctgtcca
    11581 gcgtctccag taaatcgtca tcaagcgtga tggtgactcg ttgcatttgc gttaaacctt
    11641 ttctgtggtg cgacggcgca cggggaatgc gggtaatacc gcgttttgta gcacacgtcc
    11701 ggcgtcagag gaaaaggtta atttatctcc caccacctgg gtttcgacga tttgtccgtt
    11761 gtccatcacc atcacccgct gacagaagcg ttccaccagg cgtaagtcgt gggtgatgaa
    11821 caggcaggcg gtgccaaact gttgttgcag ctttttcagc aggcgaatga cgcccgcctg
    11881 taacacgaga tcaaggttcg aaacggcttc atccagaatc agcagtttcg gttcgaccgc
    11941 cagcgcgcgt gccagacaga cacgctggag ctggccgccg cttaactgcg gtgggcgttt
    12001 gtcgagcacg ctgtcgtcga gatcgaccgc cttcagcatt tcgctggtgc gcgccagttg
    12061 ttcggctttt ttcagcgata gcaggtggcg catcggttca cgcaggatct cgcgcacggt
    12121 tttgcgcgga ttcacggcgc tgatggagtc ctgaaacacc atttgaatat cacggcggaa
    12181 cgctttacgt tgcgcgcgat tgagcttcgc caacggttcg ccgcgccagc taatatttcc
    12241 ctgtgagggc gactctaacc ctaccagcag ccgcgcgagg gtactttttc cgcagccgct
    12301 gcgtcccagc agggcgacgg tttcaccgct tttcagggcc agggaaacgt tattcagcac
    12361 cgcctggtgc tgatgttttc cgctaaatcc accgtgcgca tagtgatggg aaaggtcgga
    12421 gacgttaagt aaagtcatga tgccagctcc ataccgtaga gggcgagatg cgcggaaacc
    12481 aggctgcgcg ttaccgcatg tttgggggcg ttaaacagcg tttctacatc gccctgttcg
    12541 acaattttac cgtgagacat taccgccaca tcgtctgcca gacgcgctac tacgcccata
    12601 tcatgggtaa ccagcagcat tcccggtgct tgtttttgca taatgctttc cagtagatcg
    12661 aggatgcgtg cttgtgctac cacgtcgaga tcggtggttg gttcatcggc gatgataaac
    12721 ggtgattcgc acagcaccgc catcgcaatc atcatgcgct gcaacatgcc gccgctcatc
    12781 tcgaacgggt agagcttcag cacgcgcgcg gcgttttcca gccccaccgc ttctatggca
    12841 gcggtaagcg tagcgtcatc ggcgggtttc cccagcgcca ggcaggtttc acgcgcgtgg
    12901 gtgtgcatgg tgtgcagcgg attaaaggca ctgcgcgggt tctgcatgat ggtggcaatt
    12961 ttgatgccgc gcagggcgca gggagaaacc ggttttccat cggccaaaat ttcccccgcc
    13021 gtctggcgaa cgcctgcggg cagaatgccc agcgtcgcgg cgcaggtcag cgacttcccg
    13081 ctgccactac cgccgactaa cgccagcaca cgcccacgtt gcagggttaa cgatacgccg
    13141 tgcaccagcg gctgcgcggc ctgtagcgcg atattacgga gttcaatctg ttgtggcatt
    13201 agtgtgcgtg ctccgtcacc agatgagggt ccagatgatc gcgcagtgcg tcacccacca
    13261 ggttaaaggc catcacgctg ataaacagcg ccagccccgg ccagaacatt tgcagcggct
    13321 gggtccagat atactgacgc gcgtcgttaa tcatcacgcc ccattcggcg gtcggcgcgg
    13381 tcacgccgag accgaggaag gacatccccg cgacgtgtag catcatatga ccgatatcca
    13441 gcgtcgccag caccagcagc gaggggatca ccgcacctgc cagatgatca ataaacaccc
    13501 gcacatggcc cgcgccagaa agccgtgatg ccagcacaaa ctcgcgctgg cgcagtgaaa
    13561 tcaccagact gcgcaccatg cgcgcgtacc atgcccagtg cgaaagggcg atagcgataa
    13621 ttacgttggt caacccggtg ccaagcacgc cgaccataaa gaacgacaga atcgaggttg
    13681 ggaaggtcat aaacatatcc gcgacgcgca tggtggcctg atcaacgcgc ccgccaatca
    13741 gcccggcgct gccgccaata atcagcccta atgtcagcac caacagcagg caggccatta
    13801 ccgagccgag cgacacgcgg gtcgctgcca tcagccgcga gaaaatatcg cgccctaaat
    13861 gatcggtgcc cagccagtgc tgcgcatccg gcgagagcag gcgcgacggc aaatcaatag
    13921 cctgagggtc atacggcagc caccactggc tggtgagcgc aatcagcgcc agcagggcga
    13981 taatgatcag cgccaggcga accgaccagc gggaggagag gaaaaagttc acgcgtgcgc
    14041 tccttcatga cgacgaatac gcgggtccag cgcggcgttg agcaaatcga caatcaaatt
    14101 acagaccaca aaaaccacca ccatcatcag cgtaaagcac tggatcaccg gatagtcacg
    14161 gttaaaaatc gccgacaccg catagcgccc gacgcccggc caggcaaaga tgttttcgat
    14221 aatcatcgtc ccgccaatca gttcgccaat gtgcatcccc acggcagtga tcatcggcag
    14281 cgaggcattg cgcagaatat ggcggcgttc ggtctgtttg tcattcaggc cgcgcagccg
    14341 cgcccaggtc acatgacgct gaccggcgac gtccagcata ctggcgcgca gcaaacgcgc
    14401 gttaatcgcc agcgacataa aggcgatgga taccgcaggc aaaatgatgt gctgccagcc
    14461 gccgtaaccc atcgcgggta gccattgcag atacaccgaa aacgccatca ccagtaaaaa
    14521 cgccagccag aagttaggca tcgacacgcc gagaaacgcg atgaaacgga cggcgaaatc
    14581 gggcaagcga tcgcggtggc gcgccgccca gataccgagc ggcacggagg tgagcagaat
    14641 taataccagc gccgcacccg ccagttccag cgtggcgggc aggaagttca gcatatcgtc
    14701 cagcaccggg cgttggctgg cgaatgagat accaaagtca agatgcagcg ccttccatag
    14761 ccaggtgcca tactggacgt acagcggctg atccagcccc agcatagtgc gggtagaggc
    14821 cagcatctcc ggcgtcggcg gcaggttaga taaacgcaaa taatcaagcg ccgggtcgcc
    14881 ggtgccgagg cgcagcatca aaaaaatgat caccgaggcg gcgagcacca tcgggatcag
    14941 cagcagaaag cggcgtaata cataacgcaa cattaaggtt tcaccggttt aatctgttcg
    15001 aacgggattt cggtggcgat cggcgcgtag gggatgttac ccagttccgg ttttgaaacc
    15061 accatcattg agatgtaact gataggcaga taaaccgcct cgtcatgcag acgggtcaga
    15121 atgtcgcgat acagcgcctg acgttgcgtt tcgtcatggg tcgccagcac ttcgccgatc
    15181 tctttatcaa tcagcggctt gtcggctaaa ccttgctgtg cctggaagtc agcgtgcgac
    15241 ggtacgcgca ttgaactcag gaaggcgtgt ggatcgtatg gcgcgcccca ggtgcggtgg
    15301 aaaatcatgc caaaacgacc gtcgcgctga cgagcataga tactgctctc ttcttcgcca
    15361 atcagcgaga catctgcgcc aatctggcgc atatcagcct gaatgatttc cgccatcgat
    15421 ttgcttaacg catcggtgcc gatgaacgac agttcaatgc gcagcggctg accatttttc
    15481 tcgcggatgt ctttgccagc aggcagcgtc caaccggctt tttccagcaa cgctttcgct
    15541 ttctgcggat cgtactggcg cggtttcagg ccgaggttgg cgtagggcac agaaggggca
    15601 aacagggtgt cggcgacctg ctgggtacca tacaacgcgt tatcaatcag cgattttttg
    15661 ttcaccgcgt aattaagagc ttcacgtact gccagctcgt tggtgggggc tttggcggta
    15721 ttgagcgcca gcatcacggt ttcgatcggc tgtgacagtt gagtgtggta agccgggttc
    15781 tggctaaagc gggcgaaggt atcgagcggt aataaccctt cgtttccgta cagcaggtcg
    15841 atatcgccag tttcaaacgc caccgcgcgg gtagtcgggt ccgggatcac gttaaaggtg
    15901 atctttttaa tcgccggttt ttcgccccag tagttttcgt tacggacgaa gacatcgtac
    15961 tgattcagtt tcgattcctg caaaacccac ggtccggtgc caatcggcgc ttttatcccg
    16021 ttcatggttt cgtgattttt aaattgtgac ggggcaataa agcggaaagg acgcggcagg
    16081 gccagttctt gcaggaaagg atagtaggcg cttttcaggg tgatttgcag ctcgtttttg
    16141 ctgagtgctt tgacatcaac aatctggttt gccagctcca gccaggcgtg acgttgacgg
    16201 ttatcgagca ctgcacggaa gttttctgcc gccgcctctg catcaaacgg ttcgccgttg
    16261 gagaatttca cgtcatcacg caaggtgaag gtccaggttt tgccatcttc cgaatgggtc
    16321 cagctttttg ccagccacgg gatcaccgaa ccgtctgcct gatatttcac cagcggttca
    16381 taaaccatgc tctgggcgaa catttggtta ggcgtgtaaa ggtgcgggtt tagtggcccg
    16441 acattcaccg gccaggcggt ggtgatttca tctggtgcag cggcatggac gataaaagac
    16501 gcacaagcca gcagcgcaaa tagagtgcgg cggagtgtag agaacatggt aaccccaatg
    16561 gattaaaata gatggcggaa ataagtatga cgattttaag tattcgtcat actgattacc
    16621 tgttctggat caatagatgg gcagtcgggt gggcggatca gttaactgaa tcgatccatt
    16681 gcacactgtc ggcggtgagc gtaaaggggg taggggtgca gatcgccagg cttaaatttt
    16741 ctagctgaca atggctgact gacagcgagg agttataggt gctgtctacg ctgacgattt
    16801 gccaggcgct gccgccacgc tgtttaacga tggcttcttt gcgcgtccag atgcgccaga
    16861 acatttccag ttgctgatca ggatgcacgg cgtccatctc ggcatgttcc ccgaggctga
    16921 atacggcgtt cgccagccag cgccagttgg cgcgcgggcg aatcacttcg atatcgcagc
    16981 cgacttcgcc ttcatcgctc aacagcaggg cgatatcgtc accgctatgg cttaagttga
    17041 accatagcgg cgtttccggc gcgaatgcag gtttgccttg ttcgccatag atgatctccg
    17101 gtagcgggga aagcgtgtgc gaaagcaatg cacgccccgc cagccagcgt tcgcgtcgtg
    17161 gaccttgcgg tgcttgctcg cgtaaaccca gtggcagagg agctgcgctt aaggtcgaaa
    17221 ctttccccag aactatccga tacatatcag ggccaacgtt taatggaaaa tgaaagtgcg
    17281 tatcgtaaca cttgtcgcct catcccggta accgactttt cggtctgcct ggccccagta
    17341 aaatcgccag tttgctgccg cctttggtgg tttccatcca gattttacac acgctggtta
    17401 atggcaccga aagtagcatc cctaccgggc cgagcagcca tccccaaatt aacaatgaaa
    17461 gaaataccac catggtggac atccccaggc gatggcccat catccgtggt tctaaaatat
    17521 tgccgatgac catatggacc actaaaaaca atgcgccgac cagaatacat tcgtaaatac
    17581 cattaaacag cagcacctga atcattggcg gtacggcgga aattaccgca ccgatattgg
    17641 gcacgtagtt gagcaaaaac gccagtactg cccacatcag cgcaaactgc acccccatca
    17701 gctccaaccc cagccagacg attacgcctg tccataaact gagtagcgtc tttaacgcca
    17761 gataatgcga aacgccttta agtgcgcggt gtagtccggc gatgtgaatc tgtggattat
    17821 tcaacgcgaa acgcattttg taagggacgt ggcgcacttc aaacagcata aaaactacgg
    17881 tcatcaccag caaaagcacg ctcgccattg ccccggaaag cccggtcatt agcgccgtgg
    17941 tgaaggtaac cactttttcc gagtccatcc gctgtagtat tcgctccggc gacatatgca
    18001 aattaagaaa aggcaacatc tcctgcaatt taaaaagttt gcgcgtcagc tccttattaa
    18061 acttcggcag catagagata aattcgttaa acgatgccgc cagtacgccg accagcgcgg
    18121 tgagtgcgat cagcatcacc acaaccacaa tcgtaatggc aacggggcgt tgtactcccc
    18181 gacgaataaa ccaggtgacg agcgggttga gaacgatggc aaaaaacagc gccagtaata
    18241 gctgcacaat gatatctgcc gctgcgtgaa tgcccgcgag gatcactacc agcgaggcca
    18301 acttgagcag aatgcgcatg cccgttttat cgggttgagg ggtttccatt ggggcttcct
    18361 tgtgactttt tgtattaagt gtagcgggag tcacgccagc aatattctga ttcttactgc
    18421 tgattttcac gtcagcacat ggtaaaaatg aaacactgtt gtaaaaatgt ggtgatcctc
    18481 atgcccgaac ccgtagccga acccgcgcta aacggattgc gcctgaattt gcgcattgtc
    18541 tccattgtca tgtttaactt cgccagctac ctcaccatcg ggttgccgct cgctgtatta
    18601 ccaggctatg tccatgatgt gatgggcttt agtgccttct gggcagggtt ggttatcagc
    18661 ctgcaatatt tcgccacctt gctgagccgt cctcatgccg gacgttacgc cgatttgctg
    18721 ggacccaaaa agattgtcgt cttcggttta tgcggctgct ttatgagcgg tctgggatat
    18781 ctgacggcag gattaaccgc cagtctgccc gtcatcagcc tgttattact gtgcctgggg
    18841 cgcgtaatcc ttgggattgg acaaagtttt gccggaacgg gatcgaccct gtggggtgtt
    18901 ggcgtggttg gttcgctaca tatcgggcgg gtgatttcgt ggaacggtat tgtcacttac
    18961 ggggcgatgg cgatgggtgc gccgttaggt gtcgtgtttt atcactgggg cggattgcag
    19021 gcgttagcgt taatcattat gggcgtggca ctggtggcca ttttgttggc gatcccgcgt
    19081 ccgacggtaa aagccagtaa aggcaaaccg ctgccgtttc gtgcggtgct tgggcgcgtc
    19141 tggctgtacg gtatggcgct ggcactggct tccgccggat ttggcgttat cgccaccttt
    19201 atcacgttgt tttatgacgc taaaggttgg gacggtgcgg ctttcgcgct gacgctgttt
    19261 agctgtgcgt ttgtcggtac gcgtttgtta ttccctaacg gcattaaccg tatcggcggc
    19321 ttaaacgtgg cgatgatttg ctttagcgtt gagataatcg gcctgctact ggttggcgtg
    19381 gcgactatgc cgtggatggc gaaaatcggc gtcttactgg cgggggcggg gttttcgctg
    19441 gtgttccctg cattgggcgt agtggcggta aaagcggttc cgcagcaaaa tcagggggcg
    19501 gcgctggcaa cttacaccgt atttatggat ttatcgcttg gcgtgaccgg accactggct
    19561 gggctggtga tgagttgggc gggcgttccg gtaatttatc tggcggcggc gggactggtc
    19621 gcaatcgcgt tattactgac gtggcgatta aaaaaacggc ccccggtgga aatacctgag
    19681 gccgcctcat catcttaaaa cttactgaat aaccagcgta ttaatgatgt tttctgcggt
    19741 ggtctgtgct ttttgctgat tgtcagcggg cagcgtaatt tgcatggtta gcagttgatt
    19801 acccacgtta cccagaataa cggaagagta cgccgtctgg cctttcgcgg agataatgct
    19861 gtctaactgc tgcattttgt gacctttcag ctcaatggct ttattggtta ccacttgcag
    19921 ctgcggatcg cggctacgtt gctgatcttc cagacgcttc gccagcaccg ccagatcttc
    19981 tttcggatca tcgcccatga tgacgatgac tgctttctgc ccggtggcgt cggaccagac
    20041 atgcatgtta ttggcctgcg ttcccagctt accgctctgg tcggtcatat ccgctggcag
    20101 cgagaaactt aacttgccat caagcaggtt gacgggattc ccggtcgcgt tactttctgc
    20161 gaccgaaccc tgcgccgtag cgttagtgtc tttatcatca caggccgcaa gccccataac
    20221 cagcaggcca attccgacat atttaaccag attgcgcatt gacttcttcc tttcgataaa
    20281 cggccataac ggctcattca tccatcttat cacaactctg ataacgaacc tttaactcgc
    20341 ctgcaaagcg ttgatttcgg atttatctgc cagtcttttc aacagcatat tgagtaatac
    20401 gccatacatt ggcaggaaga aaacgatact gattaacact ttgaaacagt aatcgaccag
    20461 cgcgatttcc atccagtgtt cagccataaa ggcatccggg ctacgccaga aggcaatgaa
    20521 gaagaaggcc agcgtgtcgc tgacgttacc gaacagtgtg gacgctgtcg gtgccagcca
    20581 ccagtgacga ctctgacgca ggcggttaaa aacgtgcacg tcgaggatct gccccagcgc
    20641 gtaggccatg aaactggcgg tggcgatacg ggcgacaaac aggttgaagt gggcgagcgc
    20701 gccgaatcct tgccatgacc ccatataaaa cagcgatgag atgacgtagg agatcagcaa
    20761 tgcggggatc attaccgcaa agatgatgcg acgagccagc ggcgcgccaa aaatacgcac
    20821 ggtcaggtca gtagcgagaa agataaacgg aaagctaaac gcgccccagg tggtatgaaa
    20881 accaaaaatg gagaccggaa gctgaaccag atagttactg gaggtgatca ccagaagatg
    20941 aaataacgat aaccagaaca acgccttata gcgttgcgtt tgcgagaaaa cgttcatatt
    21001 gtaccttttt gattaaccat tggggtgagg gaacccaata cgtgcgacac gctattttat
    21061 acgtgctgac ccttttttcg agccgccgca tgatactgct ttacgttgac aatgcaatgg
    21121 ttaattttca cgcaatcgtt aacctggttt gcttacggac tcacagggcg taaactagcg
    21181 ccgttttttt atgtgatgag aagaaaatga ccgatctctt ttccagccct gaccacacac
    21241 tcgacgcact tggcctgcgc tgcccggaac cagtaatgat ggtgcgcaaa acagtgcgca
    21301 atatgcagcc aggtgaaacg ttgctgatta ttgctgacga tccggcgacg acccgcgata
    21361 ttcctgggtt ttgtaccttt atggaacacg aactggttgc taaagagacg gatggactgc
    21421 cttatcgtta tttgattcgt aaaggcggtt gatgggggaa gattggcttc gatgccgcct
    21481 tttcccctca ccctaaccct ctccccagag gagcgagggg accgatcgcg ctcaatgttg
    21541 cgatcggttt gccttatttc ctgcgcaaca gccttaacgc attcgccgtt accagcaccg
    21601 tcgcaccggt atctgccagc actgccagcc acagtccggt catccctaac agcgtcgtga
    21661 cgaggaaaac gcctttcagc cccagcgcaa tagtgatgtt ctggcggata ttggcgtgag
    21721 tggcgcgtgc cagttcaatc atttgcacca gaccgcgcag gtggttatgg gttaatgctg
    21781 catcggcggt ttccagcgcc acgtctgtgc cgctacccat tgcaatcccg atggcggcag
    21841 ctttcatcgc tggcgcgtcg ttaataccat caccgaccat cgccagcggc gcatgttgat
    21901 tcagctcggt caccgctttg actttatctt ccggcaagag gcccgcttta aattccagcc
    21961 ccagttcccc ggcgattgcc gctgctgcgc gtggattatc gcctgtaagg atcacccctt
    22021 tgacgcccag cgcgttcagt tcactgatag cagttgcagc atcggcgcgc agggtatcct
    22081 gcaacgcaat gacacccagc acgtcatcgt tacgtactac cagcactacc gtttgcccgg
    22141 cgctttccag ttcattaatc aaaccagcaa atgcatcagc aggatgtttt ccggcagcgc
    22201 atatcaacac gcgctcaccg ttaacctgcg cttcaatgcc agacccgacc agcgcccgct
    22261 gtgattgggc ggtaggaatg gcgagttcag caacctgtgc ttcgcgcaca atggcctgtg
    22321 ccagtggatg cgtcgcgcct tgctcgaccg ccgccgccag tgtcagcagt tcagattcac
    22381 taatacccgt tgccggatga atcgcggtaa cgcgcggttt accgacggtc agcgtaccgg
    22441 ttttatcaaa cgccacctgg gtaacacgac ccagctgctc cagcgccgcg ccgcctttaa
    22501 tcaacgcccc acgacgcgct gctgccgcca gcccggaggt aatcgccgcg ggcgtggaga
    22561 taactaaagc acacgggcaa ccaatcagca gcagcgtcag ccctttataa atccactcct
    22621 gccagctggc ggcaaacagc agcggcggca ccagcgttac cagcagggcg accgccataa
    22681 tcgctggcgt gtaaatacgg ctgaaacggt cgataaaccg ctcaataggt gcgcggcgct
    22741 cttcggcttc ttcaatcagt ttcagaatcc ggtcaatggc gctggcaccc ggttctgaca
    22801 gcacttccag cgttaccaga cggtctacgc tggtggcgcc tgcaggaact ttatcgcccg
    22861 tcgcgcgctc caccggaatg gattcgccgg tcagggcact ttcatcaaaa ctggcaaacg
    22921 gtgagagcag tttaccgtcg gcaggcaaac gcccacctgc cgcaacttca atcacatcgc
    22981 caggccgcag gctgtttatt gccacctctt cacgctcacc gttactcaga cgcgtggcgg
    23041 tttctggctt cagcgccatt aacgcgctga ccccttgacg cgcgcgactg gcggcccagc
    23101 cttccagtcg ttcaccaatc aaaaacagca gcaacaccat cgcagcttca gccgttgcgc
    23161 caataaacag cgccccagta gcggctacgc tcattaaggt ttcaatggcg aagtagctgc
    23221 cagatttgat cagccgtaat gcctgacgag caattgggta cagcccaacc agcgtggtcg
    23281 cgataaacgc cagttgacca aacggatgat tgaactgctc cagcccccaa ctgattgcca
    23341 tcatcacgat aagcgtaatc agcggcagat tctctttcag gcgtgattct tgtggttcgt
    23401 cggaggcctg ttcatcgcgc agggaatagc ccgctttttg caccgcagat tcaacttgtg
    23461 cccggatgtc attgtcggca tccaccacca gtttttcggt ggcgaacaac acctgtacct
    23521 gattcacgcc tgcaagctgg cgcacggcat tttccacttt gcgcgcacag gcggcgcagt
    23581 ccataccgct gactttccag ctatagcggg tgccggagac gttttcagag agagttggcg
    23641 tgctggaaca tgcgccgtcg cagcaacagt cgttggtgtt ctgtaccgta gttagcggtt
    23701 tgaacgcagc aaattgaggg gctttcttgc cgtgattgtc aggagtcgac atggcatcct
    23761 ccggttaagt tttttctcat taaccgaagg atacactctg gagtcgactc cagagtcaag
    23821 ttttatcaga gatacagcga gcggacgatg aggaagtgac cagcaaagta gcaggccgca
    23881 gcaatcgcgt tatccgcacg gaagcgtcgg cgatagtgac tacccagcca gacaaagtta
    23941 ctgataaaca gcaacgaagc gccaacaaac gcagagagcg ccggagcggt cggacggaag
    24001 aaccacagtt cacctgccag ccacaccatc accagcgtca tgccgataaa cgtgcagata
    24061 ggccaacggt actcttccag gcgcgtccag ataatcgcca gtaacagcgc gcccagaacc
    24121 agcagcacca gtggcagagg ccagaagaaa gagagcgtca tctgactggc gaaatagatg
    24181 gtgtacagca ggtgcgagag gaaaaacgcg ccgatggcgt acatcagacg ttgacgtggc
    24241 aacagggtta gcgcatcgcc cagcagtgag gcacacagcc ccgccagcac cagatagcta
    24301 atggcgtcga acatcggcgc ttgccaggcc agcaacagca ggagaagaag ggttaacggt
    24361 ttaaacaccc agcgttgcca ggttggccca cgatacgatg catccacaga tagccatgcg
    24421 gaaagacaga cagcgataaa cgaccaaagc atcttagttc cttgaattcg ttattttttc
    24481 gtaagcggaa tgcctacggt cttgcttcca gtttagtggc gagggtggac ggatgacaac
    24541 accataactg gcagggtatg cttatttccg cattttccga tgagggataa agatgagtaa
    24601 accacctctt ttctttattg ttatcattgg cttaatcatc gtcgccgcat cgtttcgttt
    24661 tatgcaacaa cgacgggaaa aagctgataa tgatatggca ccgctccagc aaaagctggt
    24721 ggtggtgagc aacaagcggg aaaaaccgat taacgatcgc cgttcgcgcc agcaggaagt
    24781 gacgccggca ggcaccagta tgcgctatga ggcaagcttc aaaccgcaaa gcggaggaat
    24841 ggagcagacg tttcgcctcg acgcccagca gtaccacgcc ctgacagtgg gcgataaagg
    24901 tacgctgagc tataaaggaa cgcgctttgt cagctttgta ggcgaacaat aaggttattt
    24961 tttaacttta aatttcttct gccagaccag cagttcaaac acgccgaaaa ggaaaatccg
    25021 caccttttcg gcggtggtca tctgcgggcc atctttcggt aaagtggatt tcaatagcgc
    25081 cagctgcata ccatgcatca gcacggtaaa aatcagcgcc acgttaacga aaatattcag
    25141 cgggcgtggg aagggatgca ccaggttgag gattaaaaat ccccaaacac agagcattaa
    25201 caaacgacca atattaatca gcatcacttt ctccttgtgc ttcgcgttga tacagccgat
    25261 aagccacctg acccgccact ttttcccgat gtaatgacca gtttgccgga acagtgggca
    25321 gacccttttc gacttcgctt tcgacataaa tcagggcttc gtcagccagc cagccgttat
    25381 cttccagtaa atttatcgtc tcttctaaca agccacggcg gaacggtgga tcgacaaaca
    25441 cgatattatg cggtgtgcct ttttgcgcca ggaatgacat cgcgttgctg ttcaccacgc
    25501 gtgcattgcc tgcttttagt gtcgccagat tcttaattaa ctgctgagaa accgcacgat
    25561 ccatctcaat caacgtggcc cccgcagcgt agcgcgataa cgcttccagc cccagcgcgc
    25621 cgctcccggc gaagcaatcc agacattggg cgtcaacaat gaccggagcc agccagttaa
    25681 acaacgtttc gcgtacgcgg tcggtggtgg ggcgcagacc tggactatca ggaaccggga
    25741 gtttacggcc tcgccactgc ccgccaataa tgcggatttg gccgctgccg gaatgattcg
    25801 gttttttcat gataaattgc tcaatccgcc agataacaca tacttgcagg cggtgatgtg
    25861 aattaagtta agtgatagac tatttcatca tttttttagc tgctatgtac atagcgttaa
    25921 cgctgtgcca tgaagcaaca gcgaggagtg tagtcgcaaa tggcgaaaga aaaaaaacgt
    25981 ggcttttttt cctggctggg ctttggtcaa aaagagcaga ccccggaaaa agagacagaa
    26041 gttcagaatg aacaaccagt tgtagaagaa atcgttcagg agcaagagcc tgtgaaggct
    26101 tctgaacaag ccgttgaaga gcagccgcag gcgcatactg aagccgaggc ggaaactttt
    26161 gctgccgacg ttgtggaagt tactgaacag gttgctgaaa gtgaaaaagc gcagcctgaa
    26221 gcggaagtcg ttgcacagcc ggaaccggtc gtagaagaaa cgccggagcc agtggctatc
    26281 gaacgtgaag aactgccgtt gccggaagac gtcaacgccg aagcggtttc gccagaagag
    26341 tggcaggccg aagcggaaac cgtagagatt gtcgaagcgg cggaagaaga agcggctaaa
    26401 gaagaaatca ccgacgaaga gccggaggca caggcgctgg ctgccgaagt ggcagaagag
    26461 gcggtaatgg tggtttctcc ggcagaagaa gatcagccgg tggaagaaat cgctcaggag
    26521 caggaaaaac cgaccaaaga aggtttcttc gcgcgcctga aacgcagcct gttaaaaacc
    26581 aaagaaaatc tcggttccgg atttatcagc ctgttccgcg gtaaaaaaat cgacgatgat
    26641 ctgtttgaag agctggaaga acagctgttg atcgccgatg tgggcgtgga aaccactcgt
    26701 aaaattatca ccaatctgac agaaggcgca tcccgcaagc agcttcgtga tgccgaggcg
    26761 ctctatggcc tgctgaaaga agagatgggc gagattctgg cgaaagtcga tgagccgctg
    26821 aatgttgaag gtaaaacgcc gttcgtgatc ctgatggtgg gcgtcaacgg tgtgggtaaa
    26881 accacgacga ttggtaagct ggcgcgtcag tttgagcagc agggtaaatc ggtgatgctg
    26941 gcggcaggcg atactttccg tgcggcagcg gttgaacagc ttcaggtctg gggtctgcgc
    27001 aacaatattc cggtgattgc ccagcatacc ggtgcggatt ccgcttctgt tatcttcgat
    27061 gccattcagg ccgctaaagc gcgtaacatc gacgtcctga ttgccgatac cgccggacgc
    27121 ctgcaaaaca aatcgcacct gatggaagag ttgaagaaaa tcgtccgcgt gatgaagaaa
    27181 ctcgacgttg aagctccgca tgaagttatg ctgaccatcg atgccagcac cgggcagaat
    27241 gcggtaagcc aggccaaact gttccacgaa gccgttggct taaccggcat cacgctaacg
    27301 aaactggacg gcacggcgaa aggcggggta attttctcgg tggctgacca gtttggtatc
    27361 cctatccgct acattggtgt cggcgaacgt attgaggatt tgcgtccgtt taaggcggac
    27421 gactttatag aggcactttt tgcccgagag gattaacaat gattcgcttt gaacatgtca
    27481 gcaaggctta tctcggtggg agacaggcgc tgcagggcgt tacgttccat atgcagccgg
    27541 gtgagatggc gtttctgacc ggtcattccg gcgcagggaa aagtaccctc ctgaagctga
    27601 tctgtgggat tgagcggccc agcgccggga aaatctggtt tagcggccat gacatcacgc
    27661 gtctgaaaaa ccgtgaagtt ccgtttctgc gccgccagat tggcatgatt ttccaggatc
    27721 accatctact gatggaccgt actgtctatg ataacgtggc gatcccgctg attatcgccg
    27781 gtgccagcgg tgatgacatt cgtcgccggg tgtcggcggc gctggataaa gtcgggctac
    27841 tggacaaagc gaagaacttc cctattcagc tttccggcgg tgaacaacag cgtgttggca
    27901 ttgcccgcgc ggtggtgaac aagcccgcgg tactgctggc ggacgaaccg actggtaacc
    27961 tggacgacgc gctgtcggaa ggcattttac gtctgtttga agagtttaac cgcgttggag
    28021 taaccgtatt gatggcaacg cacgacatca acctgatttc gcgacgttcc tatcgcatgc
    28081 tcaccctgag cgatggtcac ttgcatggag gcgtgggcca tgaataagcg cgatgcaatc
    28141 aatcacattc ggcagtttgg cgggcgtctg gatcgcttcc gtaaatcggt cggcggctct
    28201 ggcgacggcg gtcgtaacgc gccgaaacgc gcgaaatcct cgccaaaacc ggtaaatcgc
    28261 aaaaccaacg ttttcaacga acaggtacgc tatgcctccc acggcgcatt gcaggatctg
    28321 aaaagcaaac cgttcgccac gtttttaacg gtgatggtta tcgccatctc cctgacgctg
    28381 cccagcgtct gttatatggt gtacaaaaac gttaaccagg cggcgacgca atattatccg
    28441 tcaccgcaaa tcactgttta tctgcaaaaa acgctggatg atgacgccgc tgcgggcgtg
    28501 gtggcacagt tgcaggccga gcaaggcgtg gagaaagtga attatctttc tcgtgaagac
    28561 gcactgggcg agttccgtaa ctggtctggt tttggtggtg cgctggatat gctggaagaa
    28621 aacccgcttc cggcagtggc ggtggtgatc ccgaaactcg attttcaggg gacggagtca
    28681 ctgaatacgc tgcgtgatcg tatcacgcag attaacggca ttgacgaagt gcggatggat
    28741 gacagctggt ttgcccgcct ggcggcgttg accgggctgg tcgggcgcgt ttcggcaatg
    28801 atcggcgtat tgatggtggc ggcagtattc ctcgtcatcg gtaacagtgt gcgcctgagt
    28861 atctttgctc gccgtgattc tattaacgtg cagaaactga ttggtgcgac ggatggtttc
    28921 atcctgcgtc cgttcctgta cggtggcgca ttgctgggat tttctggcgc attgttgtca
    28981 ctaattttgt cagaaattct ggtgctgcgt ttgtcatcgg cggttgcgga agtggcacag
    29041 gttttcggaa cgaagtttga tatcaatggc ttatcgttcg atgaatgcct gctattgctg
    29101 ctggtatgct cgatgattgg ctgggtggca gcgtggcttg ccacggtaca acatttacgc
    29161 cactttacgc ctgaataata aaagcgtgtt atactctttc cctgcaatgg attccgtagc
    29221 agggaaagag accccgttgt ctcttcccgg tattttatct ctatgtcaca ttttgtgcgt
    29281 aatttattca caaacttgca ttgaacttgt ggataaaatc acggtctgat aaaacagtga
    29341 atgataacct cgttgctctt aagctctggc acagttgttg ctaccactga agcgccagaa
    29401 gatatcgatt gagaggattt gaatgactga caaaatgcaa agtttagctt tagccccagt
    29461 tggcaacctg gattcctaca tccgggcagc taacgcgtgg ccgatgttgt cggctgacga
    29521 ggagcgggcg ctggctgaaa agctgcatta ccatggcgat ctggaagcag ctaaaacgct
    29581 gatcctgtct cacctgcggt ttgttgttca tattgctcgt aattatgcgg gctatggcct
    29641 gccacaggcg gatttgattc aggaaggtaa catcggcctg atgaaagcag tgcgccgttt
    29701 taacccggaa gtgggtgtgc gcctggtctc cttcgccgtt cactggatca aagcagagat
    29761 ccacgaatac gttctgcgta actggcgtat cgtcaaagtt gcgaccacca aagcgcaacg
    29821 caaactgttc ttcaacctgc gtaaaaccaa gcagcgtctg ggctggttta accaggatga
    29881 agtcgagatg gtggcccgtg aactgggcgt aaccagcaaa gacgtacgtg agatggaatc
    29941 acgtatggcg gcacaggaca tgacctttga cctgtcttcc gacgacgatt ccgacagcca
    30001 gccgatggct ccggtgctct atctgcagga taaatcatct aactttgccg acggcatcga
    30061 agatgataac tgggaagagc aggcggcaaa ccgtctgacc gacgcgatgc aaggtctgga
    30121 cgagcgtagc caggatatca tccgcgcgcg ctggctggac gaagacaaca agtccacgtt
    30181 gcaggaactg gctgaccgtt acggtgtttc cgctgagcgt gtgcgtcagc tggaaaagaa
    30241 cgcgatgaaa aaattgcgcg ctgctattga agcgtaattt ccgctattaa gcagagaacc
    30301 ctggatgaga gtccggggtt tttgtttttt gggcctctac aataatcaat tccccctccg
    30361 gcaaaacgcc aatccccacg cagattgtta ataaactgtc aaaatagcta ttccaatatc
    30421 ataaaaatgg ggtatgtttt agcagagtat gctgctaaag cacgggtagc tacgtataaa
    30481 acgaaataaa gtgctgcaca acaacatcac aacacacgta ataaccagaa gagtggggat
    30541 tctcaggatg aacataaagg gtaaagcgtt actggcagga tgtatcgcgc tggcattcag
    30601 caatatggct ctggcagaag atattaaagt cgccgtcgta ggcgcaatgt ccggtccggt
    30661 ggcgcagtac ggtgaccagg agtttactgg cgcagagcag gcggttgcgg atatcaatgc
    30721 taaagggggt atcaaaggca acaaactgca aatcgtaaaa tatgacgatg cctgtgaccc
    30781 gaaacaggcg gttgcggtgg cgaacaaagt cgttaacgac ggcattaaat acgtgattgg
    30841 tcacctctgc tcttcatcaa cgcagccagc gtctgacatc tacgaagacg aaggcatttt
    30901 aatgatcacc ccagcggcaa ccgcaccaga gctgaccgcc cgtggctatc agctgatcct
    30961 gcgcaccacc ggcctggact ccgaccaggg gccgacggcg gcgaaatata ttcttgagaa
    31021 agtgaaaccg caacgcattg ctatcgttca cgacaaacag cagtacggcg aaggtctggc
    31081 gcgtgcagtg caggacggcc tgaagaaagg caatgctaac gtggtgttct ttgatggcat
    31141 caccgccggg gaaaaagatt tctctaccct ggtggcgcgt ttgaagaaag agaatatcga
    31201 tttcgtttac tacggcggtt atcacccgga aatggggcaa atcctgcgtc aggcacgcgc
    31261 ggcagggctg aaaactcagt ttatggggcc agaaggcgtg gctaacgtct cgctgtctaa
    31321 cattgcgggc gaatcagcgg aagggctgct ggtgaccaag ccgaagaact acgatcaggt
    31381 tccggcgaac aaaccgattg ttgacgcgat caaagcgaaa aaacaggacc caagcggcgc
    31441 gttcgtctgg accacctacg ccgcgctgca atctttgcaa gcggggctga atcagtctga
    31501 cgatccggcg gagatcgcta aatacctgaa agcgaactcg gttgatacgg tgatggggcc
    31561 gcttagctgg gatgagaaag gcgatctgaa aggctttgag ttcggtgtgt ttgactggca
    31621 cgccaacggc acggcgactg atgcgaagta acagatagat aaatgaaaat acccgatgcg
    31681 ctgaactgcc atcgggtttt tttatattta tcgaaatggt tgtagaaaga ttgaacaatt
    31741 caggatatgc ttatgtacgg tgatatgccg tacatattat acggagcagg agagttcaca
    31801 tgtctgctgt taaaaagcag cgtattgatc tgcgtttaac tgacgatgac aaaagtatga
    31861 ttgaggaagc ggcagcgata tctaatcagt ccgtcagcca atttatgttg aacagcgctt
    31921 cgcagcgagc tgcggaagtg attgaacagc atcggcggat gattctcact gaggaatcct
    31981 ggacgcgggt gatggatgcg ctgagtaatc caccgtcacc aggtgaaaag ctaaaacgtg
    32041 cggcaaaacg tcttcaggga atgtaatact ttatggatga tctgacgata gagattctga
    32101 ccgatgatgc agattatgat ctacagcgat tcgactgcgg cgaggaagca ttaaatctct
    32161 ttctgacgac acatctggtt cgtcaacatc gcaataaaat tctgcgagcg tatatcctgt
    32221 gtcgcaacac tccagaacgt caggtgctgg gatattacac attatccggc agttgttttg
    32281 aacgagccgc actgccctcg aaatcgaaac agaaaaaaat tccctacaaa aatattccca
    32341 gcgtaactct tgggcgtctg gcaattgatc gttcattaca ggggcaggga tggggagcaa
    32401 cactggttgc tcatgccatg aaagttgtct ggtcagcctc tttagcggta ggtattcacg
    32461 gtctttttgt cgaggcgctg aatgaaaaag cccatacgtt ttatcagtcg ctgggcttta
    32521 tcccattggt cggagaaaac gaaaatgcgt tatttttccc gaccaaatct atagagctac
    32581 tttttacacc agacgattaa cgcttctccc agccaccctg ttgtgccgta aatcccagtg
    32641 ctcgcataaa cgccgtcatc acgccgcgat cttccacgcc agcatccgcc atccaccaac
    32701 atgaaacgcc aggattgtta cgcaaaacct cttccagcag atattgcccc acaccgcgac
    32761 gtcgggttac ttcccgcacg cgtagagaat ccagtgcccc ctcggtgccg cttaaggtta
    32821 cccgcacggc agcgagcagg cgctcgttaa aacgcgcggc gtagatgcgg tggttatcgt
    32881 caacctgtaa agaggaaggg gaatactccg gccagatctt ttgcaggtca atccggtcct
    32941 ggtcgctaaa gttttctaat cgaatgatgg tcagcttcat ggggaacccg tgtaaatcac
    33001 aaaagtggaa ccagtgtagc gaaataattt aatcggaggc tttctcttt
//
LOCUS       DACADF010000041        32749 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.041, whole genome shotgun sequence.
ACCESSION   DACADF010000041 DACADF010000000
VERSION     DACADF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.041"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            29..1582
                     /locus_tag="ISB30_001831"
     CDS             29..1582
                     /locus_tag="ISB30_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP1989469.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVDYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            1609..2079
                     /locus_tag="ISB30_001832"
     CDS             1609..2079
                     /locus_tag="ISB30_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017801221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP1989470.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            2097..2411
                     /locus_tag="ISB30_001833"
     CDS             2097..2411
                     /locus_tag="ISB30_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP1989471.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            2436..3527
                     /locus_tag="ISB30_001834"
     CDS             2436..3527
                     /locus_tag="ISB30_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009112672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP1989472.1"
                     /translation="MKENKITVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            3539..4390
                     /locus_tag="ISB30_001835"
     CDS             3539..4390
                     /locus_tag="ISB30_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP1989473.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            4471..5331
                     /locus_tag="ISB30_001836"
     CDS             4471..5331
                     /locus_tag="ISB30_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP1989474.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            5379..6311
                     /locus_tag="ISB30_001837"
     CDS             5379..6311
                     /locus_tag="ISB30_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP1989475.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            6292..6972
                     /locus_tag="ISB30_001838"
     CDS             6292..6972
                     /locus_tag="ISB30_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAP1989476.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CGEYGILR"
     gene            complement(7016..8725)
                     /locus_tag="ISB30_001839"
     CDS             complement(7016..8725)
                     /locus_tag="ISB30_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAP1989477.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLKNVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVADLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFAPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQVNVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(8846..10237)
                     /gene="xanP"
                     /locus_tag="ISB30_001840"
     CDS             complement(8846..10237)
                     /gene="xanP"
                     /locus_tag="ISB30_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAP1989478.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            10517..11722
                     /gene="gltS"
                     /locus_tag="ISB30_001841"
     CDS             10517..11722
                     /gene="gltS"
                     /locus_tag="ISB30_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAP1989479.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(11756..13837)
                     /gene="recG"
                     /locus_tag="ISB30_001842"
     CDS             complement(11756..13837)
                     /gene="recG"
                     /locus_tag="ISB30_001842"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP1989480.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13843..14532)
                     /gene="trmH"
                     /locus_tag="ISB30_001843"
     CDS             complement(13843..14532)
                     /gene="trmH"
                     /locus_tag="ISB30_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAP1989481.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            complement(14539..16647)
                     /gene="spoT"
                     /locus_tag="ISB30_001844"
     CDS             complement(14539..16647)
                     /gene="spoT"
                     /locus_tag="ISB30_001844"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP1989482.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16666..16941)
                     /gene="rpoZ"
                     /locus_tag="ISB30_001845"
     CDS             complement(16666..16941)
                     /gene="rpoZ"
                     /locus_tag="ISB30_001845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP1989483.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16996..17619)
                     /gene="gmk"
                     /locus_tag="ISB30_001846"
     CDS             complement(16996..17619)
                     /gene="gmk"
                     /locus_tag="ISB30_001846"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP1989484.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17877..19559
                     /gene="ligB"
                     /locus_tag="ISB30_001847"
     CDS             17877..19559
                     /gene="ligB"
                     /locus_tag="ISB30_001847"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAP1989485.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDVMIPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPHLMTDRLKDLATAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLV
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(19556..20173)
                     /locus_tag="ISB30_001848"
     CDS             complement(19556..20173)
                     /locus_tag="ISB30_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAP1989486.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(20425..21288)
                     /locus_tag="ISB30_001849"
     CDS             complement(20425..21288)
                     /locus_tag="ISB30_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP1989487.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            21415..22131
                     /gene="rph"
                     /locus_tag="ISB30_001850"
     CDS             21415..22131
                     /gene="rph"
                     /locus_tag="ISB30_001850"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP1989488.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22197..22838
                     /gene="pyrE"
                     /locus_tag="ISB30_001851"
     CDS             22197..22838
                     /gene="pyrE"
                     /locus_tag="ISB30_001851"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP1989489.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22875..23471)
                     /gene="slmA"
                     /locus_tag="ISB30_001852"
     CDS             complement(22875..23471)
                     /gene="slmA"
                     /locus_tag="ISB30_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAP1989490.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(23578..24036)
                     /gene="dut"
                     /locus_tag="ISB30_001853"
     CDS             complement(23578..24036)
                     /gene="dut"
                     /locus_tag="ISB30_001853"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP1989491.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24014..25234)
                     /gene="coaBC"
                     /locus_tag="ISB30_001854"
     CDS             complement(24014..25234)
                     /gene="coaBC"
                     /locus_tag="ISB30_001854"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAP1989492.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVCVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            25406..26074
                     /locus_tag="ISB30_001855"
     CDS             25406..26074
                     /locus_tag="ISB30_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP1989493.1"
                     /translation="MKNNVQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26291..26527
                     /gene="rpmB"
                     /locus_tag="ISB30_001856"
     CDS             26291..26527
                     /gene="rpmB"
                     /locus_tag="ISB30_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP1989494.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            26548..26715
                     /gene="rpmG"
                     /locus_tag="ISB30_001857"
     CDS             26548..26715
                     /gene="rpmG"
                     /locus_tag="ISB30_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP1989495.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            26813..27622
                     /gene="mutM"
                     /locus_tag="ISB30_001858"
     CDS             26813..27622
                     /gene="mutM"
                     /locus_tag="ISB30_001858"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAP1989496.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(27661..28140)
                     /gene="coaD"
                     /locus_tag="ISB30_001859"
     CDS             complement(27661..28140)
                     /gene="coaD"
                     /locus_tag="ISB30_001859"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP1989497.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRASQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLENVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28148..29425)
                     /gene="waaA"
                     /locus_tag="ISB30_001860"
     CDS             complement(28148..29425)
                     /gene="waaA"
                     /locus_tag="ISB30_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAP1989498.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            29839..30897
                     /gene="rfaQ"
                     /locus_tag="ISB30_001861"
     CDS             29839..30897
                     /gene="rfaQ"
                     /locus_tag="ISB30_001861"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAP1989499.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPANWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            30894..32018
                     /locus_tag="ISB30_001862"
     CDS             30894..32018
                     /locus_tag="ISB30_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP1989500.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            32011..>32749
                     /gene="rfaP"
                     /locus_tag="ISB30_001863"
     CDS             32011..>32749
                     /gene="rfaP"
                     /locus_tag="ISB30_001863"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAP1989501.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFGMPLRKILSFERNLL"
BASE COUNT         7830 a         8034 c         8803 g         8082 t
ORIGIN      
        1 gcttcttaat ctgtgataag ggtaaaaaat gcaaatgatt acctcgcgac aaaacagatt
       61 attacgattc cttttacctc gaagggaata tacgactatt gttacgattg ccggctattt
      121 aaatgtttcg gaaaaaacca ttcaacgtga tttacgttta cttgagcaat ggctgggaca
      181 atggagaata aatgttgaga agcgtgctgg cgcgggtgtg atgttaagcg cggagaatat
      241 tgctgatttg ctgcatcttg atcatttgtt ggtggcagaa tgtgaagaga ttgatggtgt
      301 aatgaataat gccaggcgcg ttaaaatagc gtcgcagtta ttaagtgaaa caccgaatga
      361 aacgtcgatc agtaaattgt cagaacgcta ttttatcagc ggagcctcta ttgttaatga
      421 cctgagagta attgagtcct ggcttgcgcc gttggggtta tcgttgatcc gcagcccaag
      481 tggtacgcat attgaaggta gtgaagggca agtccgacag gcaatggcat tactgattaa
      541 cggcattatt aaccataatg agccgcaagg tgtcgtgtat tcacgtctgg atcccggaag
      601 ctataaagca ttagtccatt attttggaga ggaagaagtg ttatttgtcc agtcattgtt
      661 actggatatg gaaaatgaat taagttggtc tttgggagaa ccttattacg ttaacatttt
      721 tactcatatc cttattatga tgtatcgcaa cacgcacggg aatgcgttat caagagaaga
      781 agatcaaacc aggcaatatg atgaaaatat ctttaatgtt gccagtcaga tgattcataa
      841 gatagaacaa cgaattgcac atacattgcc cgatgatgaa gtctggttta tttatcaata
      901 tatcatttca tcaggtgtgg cgattgatgg acaaaaagat gttagcatta tttcacatat
      961 gcaggccagc aatgaagcgc gtctgattac ctggcgttta attacggtat tcagtgacat
     1021 cgtggactgc gattttagtg aagacagcgc attatatgat ggcttattgg tgcatattaa
     1081 accgctgatt aaccgactaa attatcgtat tcatatccgt aatccattgc tggaagatat
     1141 taaagcagaa ttagcggatg tctggcggtt gacgcaatat gtggtgaatc aggtatttaa
     1201 aacctggggt gagaatgcgg tgagcgagga tgaagtggat tacctgaccg ttcattttca
     1261 ggctgcgatg gaacggcaaa ttgcccgtaa acgcgtatta ctggtctgtt caaccggaat
     1321 cggaacttcg catctactga aaagccgtat tctgcgagca tttcctgaat ggacgattgt
     1381 tgatgttatt tcagcagcga atttatcaca ggttttgcct gacaatatcg aactgattat
     1441 ttcgaccatt aatttgccta cagtcactat gccggtcgct tatgttaccg ctttttttaa
     1501 tgatgccgat attaagcggg tcactgaaat ggtgattacg gaaaaattac atcatgcgac
     1561 gtctcgggtc gttgaaattt aatgtcagaa ttttatgagg tatgacgaat ggacataaca
     1621 aaaatcctga atacgaatcg cgttatttta gatatgcaag cgacgaataa agcggaggcg
     1681 attgaagaat taactaatct tctcaaaaaa gatggtgcga tcgattgtcg ggaaaccttt
     1741 attaaagatg tctggcagcg cgaagcggaa ggctcgactg gttttgaaaa tcatattgcg
     1801 atcccgcatg gtaaatcttc agctgtgatt aatactacgc tggcaattgg ccgtacccgc
     1861 caggatattc cctgggaaac gctggatggc agtagcgtgc gctgtattat tttatttgcg
     1921 gtacgtcttg aagatcaaaa taccacccat atccgtttac tttcccaggt cgcgggcgcg
     1981 ctggcggatg atgacatcat agaacaattg ttagttgaga cgagtccgca aaaaatcatt
     2041 gatctcttta gtcagtatgc acaatccgat gtctgctaaa taacagggag tatgtcatga
     2101 atattgtctg tgttgccgcc tgtacggcgg gtattgctca tacctatatt gctcgtgaaa
     2161 agttaatcaa aggagcaaaa gctctgggcc ataccattaa agttgaaacc cagggaacca
     2221 ttggtacaga aaatgaatta caggcggcag atatctctgc tgctgacgtc gtgattcttg
     2281 ctgtagatgt gaaaataaaa ggagaagaac gcttcaccaa taaacgtatt gtgcgagtaa
     2341 agacagagat cgtcattaag tcgcccgttc aatttcttga aaaggtcgaa aaatcactgg
     2401 gaaacaaatg atccttatac tggagggatc ttaatatgaa agaaaataaa attacggttt
     2461 cacaggaaat taaaaaacat ttattaaccg gcatctcgtg gatgataccc cttattgtgg
     2521 ccgccgggat atgtattgcg ttggggcaag ttcttggcgg tacagatgtt ggtgaaaaaa
     2581 caggaacaat cccgtggatg cttaatcaga ttggcggctg gggaatgggg ctgattgtgc
     2641 cgctgattag cgcggcaatt gcctattcta ttgccgatcg tcccggattt gccccgggcc
     2701 tgattgtcgg ctttctctgt gggcaaatcc ataccggatt tattggcggt atgttgggtg
     2761 gtttcctggt gggctacacc attttgctac ttaaacgcta tatccgcctg ccgcagtcga
     2821 tgcaaggact catgccaatc atggtgttgc cggtattaag caccattatc ggtgggctgt
     2881 taatgatgac gctgattggt aaacccatcg cctggttaca agaagcactg attcatttgc
     2941 tcgagtcgat gcagggcgga tcgcgcttcc tgatgggggc catacttggc gcgatggcaa
     3001 ccttcgattt cggtggtccg gtcaacaaaa ccatgtcgct gttttccgat ggcttgctgg
     3061 tcagtggcgt ctacggacca gaagccgtga aatttgtcgg ctcaatcatc cctccgtttg
     3121 gcatcacact ttcgttctta ctgacacgcc ataaatacac ccgtgcggag cgcgaggcgc
     3181 tgaaagccgc gtttccgatg gggatctgca tgattactga aggagtcatt ccgatcgccg
     3241 cgcgtgacct actgcgagta gtgggatctt gtgtggtggc atcggcggtc gcgggcggct
     3301 taatcatggt gtggggcgtg gagagtccgg ttccgcatgg cggcatgttt gttgtgcctc
     3361 tgttcaccca tcctctgctg ttttgcctgg cgctgggtat aggcacggtg atttgtggcg
     3421 tgatgctttc actctggaag aaaccggtaa cggaacgcga cgaagagttt gacgaactga
     3481 gcgatcaaaa gctgaaagat gaagagatca ctttcaccct cgaataaccg gaggccctat
     3541 gtttgccgat atgaaaagta tggtgatgaa agcctggcat gaacattacg ccttgctggc
     3601 gatcaactgt atgaatctgg aaagtgctca cgcagctatc cgcgtggcgg agaagaatcg
     3661 cgctccgatc attctcaatc tctatcaggg gcatctggcg cattttcctg cacccgtcgc
     3721 ggcggcagtg gttaaaacgc tcgcggaatc tgcctcagta ccggtcgctc tggcgcttga
     3781 tcatggcaaa aatccggact gtattcgtca ggcgtttcgt gctggtttca gcggattaat
     3841 gattgatgct tctgcttttc ccctggagga aaacgtgcgc cagaccaggg cggtggtgga
     3901 actcgccgcc agcgcgcagc tctgcgtgga aggcgaactg ggccatctgg cagatgcgcc
     3961 gcgttacgac caggccgcca atgcagacct gatgacgcaa cccactgatg tggaaccgtt
     4021 tatcgcgcag acaggcattg atctactggc cgtctcggta ggcacggcgc acggcatgta
     4081 cgcgcctggc gttgtacctg ctattgattt tcagcgtctg gcggagattt tccactgctc
     4141 gagcgtgcct ctggcattac atggcggtag cggtacgccg ttcgatcagc tccagctctg
     4201 tacgactctg ggggtagcaa aaatcaacgt gggggcggcc atttttgaac gggggaaatc
     4261 cgcgctcctg cataccctct gtcacgatat atccattgaa ctggtggacg cgctgaaagc
     4321 tatggaactg gccttcgaag aagcgattac accgtattta caggcgagcg gcgctatcgg
     4381 caaagcctga ttcattttct gtcatggcct ttatgccagt gcaataacgc accggcaggg
     4441 cggactacac gctgaaactg gagatttaac atgttagttt cgatgaaaga attactgcaa
     4501 cctacccgcc agcacggctt tgccattggc gcatttaatg tggcggataa ctgtttcctt
     4561 cgcgcagtcg tggatgaagc agaagcgaca aatactccgg cgattattgc catccatccc
     4621 agcgaacatg attttgtggg cgatgccttt ttcgctcacg ttcgcgaaat tacccagcgt
     4681 agtccggtcc cgtttgtact gcacctcgat catggtgcct ctgtagagca tgttttgcgt
     4741 gcgattcgct gcggatttac ctcagtaatg atcgacggtt cgctgctgcc gtatgaagaa
     4801 aacgtggcgc tgaccgcaga agtggttcgc ctcgctcacg cggttggcgt gtcggttgag
     4861 ggtgaactgg gaaccatcgg acaaacagga acgtcggtag aaggtggtgt ttcacaagtg
     4921 acctacacag atccggcgca ggccgccgat tttattgctc gtacaggtgc cgacacactg
     4981 gctgtggcga ttggtacagc gcatggcatc tatccaaaag ggatgcagcc gaagctgcaa
     5041 atggacattt tgcgcgacat tgccggacgt attgatattc cgctggtgct acacggtggt
     5101 tcggcaaacc cggacgcgga gattgctgaa tcagttacgc tcggcgtcgg caaaatcaat
     5161 atctccagcg atatgaaata cgcctatttc cagaaagtgc gtgaaattct cgctaaagag
     5221 agctggtggg acccgaacgt catctatccg gatgcgatta acgcggcgcg ggaagtcatt
     5281 cgccacaaaa tgaaactgtt cggttcgctc ggtaaagcat cactgtacta agccagaagg
     5341 gcggggtgac ccgcctttta cgcaacaagg aacgagatat gcactatctg gggttggata
     5401 ttggtgggac caaaatcgcc gccgttgtca tggatgcgca tggctgggag attcgccgtt
     5461 accgctgccc gacgcaaaag tcgacatatc aacaatttgt ctcatgcgtt gtggcgctta
     5521 tcgagcagat taggcgggac gttcaacgac cgatgctgac agggatcgcc ttacccggca
     5581 gtatctcgcc actcactggc ctgattaaaa acgcgaatat tcaggtgatt aacggtcatg
     5641 cgttacaggc tgatttgcag caattgcttg ggcaaccggt ggtgatagcc aatgatggta
     5701 actgtttcgc gctatcagaa gcttgcgacg gtgccgggca agattatgac gtggtatttg
     5761 gtattacgct tggtacgggc tgcggcggtg gcattgccat caagcaacga ccgtttatag
     5821 gggcctgggg aaatgctgcc gaatgcggtc atatcacgtt gccaggctat acggagcagg
     5881 aagatggtcc atcagtcagt tgctattgcg gcaaacacaa ctgcgtggag tcgtttgttt
     5941 ccggcagcgg ttttagtgaa cgctatcaac agatgactgg taacttgctc actcctgcgg
     6001 cgattgtcac cctggcacaa cgtggtgatg cttgtgccat gcagcaggtg gcacgttttc
     6061 gccaacagct tgcccgcacg ctggcaacca tcgttaacgt tgttgaccct ggcgtgattg
     6121 tgatcggcgg cgggctttcg aatgtggaac tgcttatcac cgatctgaac gcagaagtcg
     6181 ctccactggt tttcaccgat caattcacca ctcccattgt aaaagcacag cacggcgaca
     6241 gtagcggaat gcgtggcgct gcctggcttg ctatgcgcaa cggagaagcc aatgaaacgt
     6301 tcacaaatta attatgttat tgataaagca catgccattg cgcagacctt ccgcgtttgt
     6361 ttgccagagt tcgcctattt tacggtggat gactggctgc agcgggaacg ggataactgg
     6421 caagaagtgg tcgacttaca gctcggttgg gatatcaccg attttggtcg tggtgatttc
     6481 aaccaaacgg gactaacact gctaacgatg cgcaacggcg cgctggggtc ggcggcatat
     6541 ccaaaaccgt atgctgagaa aatgttgcag atccaacaag atcagcagac tccctggcat
     6601 tttcacaccc acaaaatgga agatatcctc aatcggggcg gtggagactt atgcatgcag
     6661 ttggcctggg ccaacgaagc caatctctgt gatgatcaac gagtcattac cgtgagtgtt
     6721 gatggtcagc ggcgtaccat gaaacctggt gaaacgctgg tgctaaagcc ggggcagggc
     6781 atttgcctgc cgccgcgtct gtatcatcgc ttctgggcgg aaaaagcgtt tgttctcggc
     6841 tgggaaatct cgatggtaaa tgatgaccaa cacgataatt attttcttga gccgggagga
     6901 cgttttccgg cgatagaaga agatgaaccg gtgaaatggt tattgtgcgg tgagtatgga
     6961 attttgcgct aataacgggg aacgctagcg ctgatatccg ctagcgtcct tatttttact
     7021 gcaccggctc cgtcgacgta acttcaccac tggaaactac gcccgcattc atggtttgag
     7081 tcacttgctg ctttgcggca ttcacggcat gcaattgccc gctaaccgta ggtttcagcg
     7141 ggacattgac ctgaatatcg ccactggcgg taagctgaat attgccgtcg ccagtcaacg
     7201 gtaattcagg ccatccccaa tgttgcaaaa tattcaccgg cacaccgcgt ccattcaggc
     7261 tgatatgggt ctgacgttgt ggcgtttgtg aaacactggc ggtggcttcc agaatgcctt
     7321 tttcagtaaa tgcactcagt tcgctgatat tcaccgtgct gctgttggca gttagcgcca
     7381 gcgacggacg acggacatca acacgattaa acgttgcggc ggcggcattc agattcgccg
     7441 agccactcca gacgccccat ttatgatcgg taaccagcgt cagattggca ccgtaaccat
     7501 cgagcgcggt gagctgccac gggaagtcag ggtcgatatc aatgatcaga ttgcggctgg
     7561 cgctaaatct cttcagttgc aggctgttta accaaccggg tgtcgtttcc atccacaact
     7621 gttgccagtt tttgggcaag gtatattcca gtccggcaat tgccgcatca tcgaggatca
     7681 gcgttttccc gtcgcgttgc cagtttcctg acgttctgac cataccccct tcccagcggc
     7741 tggtgaactg gcgcagtgct acgccctgcg gggcaaattc cgcgtttata atcggatcaa
     7801 ataaatgcag cgagccataa ataaactcgc tggcgttcat cgacagttta ccttcctgtg
     7861 tctgccagtc atctttactg aaggtcatgt tgcgcaagct gagatcgagg tcggccaccg
     7921 cccagtccgg accttgcaaa cgagcgtcga tgacttccag gcgaccaatc tgcaacgacg
     7981 ggacagagcg taatggtgca aagaagtcgg ttagcgattt ttcgctttgt agacggatat
     8041 ccgccatgcg caggttttcc acttgccagc tgccgtcagc gttacgctgc gcgtttccgg
     8101 ttaatgtccc gcgggcgatg tcggcaccca ggttagtcag cgtaacgcga tcgttatcaa
     8161 tactgccttc aatcagtaca ttggtggcag gaacatcatt gagcgaaagc gatccggcac
     8221 taaactgaat ctgtgccttc gtacccagca ctttaccggc ttctggtgac cacggaacca
     8281 cgccgccatt tacccgctgc gcgctcagtt tccattcgct attcgggcta ttaaacgcca
     8341 tatcacgcag ttgcagacga tcggctttga acggtagcgg cgcggtctgg tcggtgagat
     8401 tcagcgtccc gttttccagc aggatggtat cgacatggcg tggttcggtc agttgccgac
     8461 tgcttagcgc aatgtcgaca ctttttgcta ccagggtcgc gggctggcca tcacggccaa
     8521 acgtgacgtt cttcagcacg atatgagatg gcgcggaaaa acggtgatcc atcgccccga
     8581 aggccagatg atagtcgcta ttctcggaaa cccatgcgct gatatgttct gctccccagc
     8641 gagtttgcag aagaaaatag aggccagcga tcaccactaa cagagcgatg agaatgtaga
     8701 gaagcagctt cccaataaat ttcatggtct tccatcccgt gaaatgcaca taagggagtt
     8761 atgcacgatt tacgcgcaat cctcaaggcg ggaatggtga aagaggtgag actgcggcgg
     8821 taattatctt accgccgcca gtgaattact gtttttctgg tgggaaaatc agattcagca
     8881 cgatggcagt aataccgccc gcggcaatcc cggaggagag caggtttttc agccattcag
     8941 gggcaaactg caaaatcagc ggctgctgag acacgcccag accgaccgcc agcgacagcg
     9001 cgataatcag aatcgcccgg cggttcagcg gctcacgaga aacgatacgc acaccggagg
     9061 cggcgatggt gccaaacatt acaagcgttg cgccgcccag aaccggttct ggaatgtgtt
     9121 gtacaaaacc gctcactgcc gggaacagac ccagcacgat caacatcagc gcgacgacaa
     9181 aaccgacata gcggctggca acaccggtta actggatcac cccgttgttc tgcccgaagc
     9241 aggagttcgg gaaggtatta aataccgccg agacaaacga gttcaggccg tttgccagta
     9301 cgccgccttt caggcgtttc atgtacagcg gaccggacac tggctgttcg gaaacgtcag
     9361 aggtcgccgt gatatcgcca atggtttcca gcgaagtgat cataaagacc agcatcagcg
     9421 gcagcagcag actccattca atgccaagac cgtaatagag cggcgttggc accataatca
     9481 gttcttgtgt catcggttcg ttgctttctg gcaacatgcc cataaaccac gccagcgcat
     9541 atccggccgc catcgcaatc accagtgagg ccacgcgtaa gtaagggtta cgttgacggt
     9601 taagcaggat aattaaggct aagaccacgc ctgccagcag cagatttttc ggtgcgccga
     9661 aggtgttatc gctcatggct gcgtaaccgc cgccaatgga ggtcagtcca acctgaatta
     9721 gcgacaggcc gataatcatc accacaacgc cagaaaccag cggcgtaata atgcggcgcg
     9781 ccagatgcag aacgcgggag atcaccatct cggtacaact tgccagcatc aacgtgccga
     9841 acaaagctgc catcatggta ggaacatcag caccaccggt cttcagcgct gtgccgccca
     9901 taatcagcgg agcaacaaag ttgaagctgg tgccctgaat agacaacaac ccggagccaa
     9961 ccggacccca ggccttaatt tgaataatcg atgccacacc ggaggcaaac agcgacatac
    10021 taataatgtg ttgcgtgtct tgtgccggta aacccagcgc ctggcagatt aatagcgctg
    10081 gcgtgatcac cgcaacgaac atcgccagca aatgctgaca ggcggcaaac agagtttgag
    10141 gaagcggcgg acgatcttca agacggtaaa tcagttcgct gttttgagtc tgcgcaaccg
    10201 gttgcgcatt ttctgactcg agggtggaaa cagacatcga aaacaatccc gtggtagcaa
    10261 agcgggcatt ttagctgacc gaaagagaaa agcaaacgtt tgccacatcc tcatctaaat
    10321 atttaaatga gataaatgta cttttttata taacttttag ttgttttctg cttaaaatcc
    10381 atgccatttt tctattttca gcgaagaatt cgaaccagga aagcatgact cgatactcgt
    10441 catatttcgc cttactgcaa atgcgcgatg aagtatgacg agtatgaaag agtgatgcgg
    10501 acacaaagga gtaactatgt ttcatctcga tactttagca acgcttgttg ccgcaacgct
    10561 gacgttgctg ctcgggcgca agttggtcca ttccgtctcc tttttgaaga aatacaccat
    10621 accggaacct gttgcgggtg gtttattagt ggcgctggcg ctgctgatac tgaaaaaaag
    10681 catgggctgg gaagtcaact ttgatatgtc cctgcgcgat ccgttgatgc tggctttctt
    10741 cgccaccatt ggcctgaacg ccaaccttgc cagtctgcga gctggtgggc gtgtggtggg
    10801 ggttttcctg attgtggttg ttggcctgtt ggtgatgcaa aatgccattg gcattggtat
    10861 ggctagcctg ttagggcttg atccgctgat ggggctgctg gccggttcta ttacgctttc
    10921 cggcggtcac ggtacgggtg ctgcatggag taaattgttc attgaacgtt atggtttcac
    10981 caacgcgaca gaagtggcga tggcctgtgc aacgttcggt ttggtgctgg gcggcttgat
    11041 tggcggtccg gtggcgcgct atctggtgaa acactccacc acaccgaacg gtattccgga
    11101 tgaccaggaa gttccgacgg cgttcgaaaa gccggatgtg ggccgcatga tcacctcgct
    11161 ggtgctgatt gaaactatcg cgctgattgc tatctgcctg acggtgggga aaattgttgc
    11221 gcaacttttg gctggtactg cttttgaact accgaccttc gtctgtgtac tatttgttgg
    11281 cgtgattctg agcaacggtc tgtcaatgat gggcttttac cgcgtctttg agcgagcggt
    11341 atccgtgctg ggtaacgtaa gcctgtcgtt gttcctggcg atggcgctga tggggctgaa
    11401 actgtgggag ctggcttcgc tggcgttgcc gatgctggcg attctggtgg tacagaccat
    11461 cttcatggcg ttgtacgcca tcttcgttac ctggcgcatg atgggcaaaa actacgatgc
    11521 ggcagtgctg gctgcgggtc actgtggttt tggcctcggt gcaacgccaa cggcaatcgc
    11581 caacatgcag gcgatcactg aacgctttgg cccgtcgcac atggcgttcc tggtcgtgcc
    11641 gatggtcggt gcgttcttta tcgatatcgt caatgcgctg gtgattaagc tgtatttgat
    11701 gttgccgatt tttgccggtt aaccgatgaa gcggcggtct tctcaccgcc gctttttacg
    11761 cattcgagta acgttctgtt tctggcatcc agcgttctat cagggctttt gcctgttgtg
    11821 ggtaacgttc gtgaatatgt cgcgccaggc gctgaacttc cgggatcatc gcctgatcgc
    11881 gcagtaaatc cgccacttta aattcagcat tacccgtctg acgtgtacct aacaattcgc
    11941 cagggccgcg aatctccaga tctttctgtg caatcacaaa accatcgttg ctgtcgcgta
    12001 gtacctgcag gcggatttgc gcggttttgg agagcggcgt tttgtagagc agcacgcagt
    12061 gagaagccac cgcgccacga cctacacgcc cgcgaagctg gtgtaactgc gccagaccca
    12121 gacgctccgg gttttcgata atcatcagac tggcattggg tacatcaaca ccgacttcaa
    12181 tgacagtagt ggcaaccagc aggtgtagct cgccttgttt aaacgatgtc atcaccgcct
    12241 gtttctcggc gggtttcatc cgtccgtgta ccaggccaac attcaactct ggtagcgcca
    12301 gtttcaactc ttcccaggtc gcttccgccg cctgcgcttc cagtaattcc gactcttcaa
    12361 tcaacgtaca aacccagtac gcctgacgcc cttcagtcat acaggcgtgg cgcacgcggt
    12421 caatgatgtc ggtacggcgg gtatcaggaa tagcgaccgt agtcactggc gtgcggcctg
    12481 gtggcagctc atctatcacc gaggtatcga gatcggcata cgcagtcatc gccagcgtgc
    12541 gggggatggg cgtggcggtc atgatcaact gatgcggatg gaagccctgc tgctggcctt
    12601 tctcccataa cgccagacgc tgatgcacgc caaaacgatg ctgttcgtcg ataatcacca
    12661 gcgccaggcc gttaaactgc acctgttcct ggaaaatggc gtgcgtacca acaatcatct
    12721 gcacctgacc gctggcgatg gcttcttgct gagccagtcg tgctttacct ttctgttttc
    12781 cggcgagcca gcctacttcg ataccgagcg gtgcaaacca gttacggaag ttattggcgt
    12841 gctgctcggc gagtaattcg gttggtgcca tcaatgccac ctgtttgccg tgcgcaatcg
    12901 cacgcaacgc ggcgagggcg gcgaccagcg ttttaccgga acctacatcg ccctgcacca
    12961 gacgcatcat cggcacatcc agcgccatat cgcgctcgat ctccgccact acgcgtgcct
    13021 gtgcgcccgt tggcttgaac ggtaaggcgg cgaggagttt atttttcagc gcgtcattgg
    13081 cgctcagcgg ctgagcatga aaacgttgtg caccggcacg taacgccaac atgctgaggt
    13141 tgtgcgccag caactcttcc agaatcagcc ggcgttgtgc cggatgctgc ccggtttcga
    13201 gatcgctaag ctgtagcgtc ggcggtgggc ggtgcaaagt gcgcaacgct tccggtagcg
    13261 tcatcattcc ttgtgacagt tccggcggca ggagttcttc aatggcgcat gtgtcgagca
    13321 gatccagcgc ctggtcggtt aatttacgca gcgtagcctg ctttacgcct tccgtagttg
    13381 ggtaaaccgg cgtgagcgtt tcctgtaact ctggcgtgct gagatcgccc tgtacgcggt
    13441 attccgggtg gatcatctcc gcaccatatt tgccgcgctt tgcttcgcca taggccagca
    13501 cgcggcggcc tgtcgccagg ctatttttca ttgccgcatt gaagttgaaa aagcgcatag
    13561 tgagaatgcc ggaaccgtcg ctgatctggc aggtcatcat ccgccgaccg ccgaaggaga
    13621 tattgcagtt cagcacttcg ccttccaccg tggcataaac acccggcagt agttcaccga
    13681 tggggtagag atgtgtgcga tcttcgtagc gcagaggaag gtgtaagagc aaatcctgta
    13741 cggtatgcag gttgattttc gccagcttgt tactaagcgc tgcgccaacg cccgttaggg
    13801 aactgagcgg gacagcatct aacaggcgac ctttcatcgc gcttaccctg cggcctgcat
    13861 ggtggaccac cagtcagcat cggcttcaat ctcgccttgc tgattaacgt gggggtaggg
    13921 cagacctttg cgttttgcga ctttcgccag caccggatag ccgccttcaa acagcaggcg
    13981 ttgttgctcg gcttccggca acatgctgtt ttcacgcagg tacatgcccg cattttgccg
    14041 ctggcgctgg gcttcgtaaa gaatgagggc tgaggcaacg gaaacattca gcgactgcac
    14101 catgccgatc atcggaatga tgatatcctg atccgccagg gccaacgctt cctgcgtgat
    14161 gcccgttttc tcctgtccca tcaaaataca ggtcgggcga gtgtaatcaa tttcgcggaa
    14221 atcaacagcg ttatcagaaa gatgggttgc cagaatctgc atgccctggc ctttcagatg
    14281 agcgacggca tcgccaatgg tgcggtgcgt tttcacctgt acccagctgt tactacccgc
    14341 agctgccgaa gccatggtgc gcatccggct gccaggccag acagcatgaa cttcatgtac
    14401 gccaacggca tctgcggtac gaataatcgc agaaacgtta tgaggtttgt ggacctgctc
    14461 catgcagacg gtcagatcag gctgccgcct ggcgagcatt tcgcagatgc gtgcataacg
    14521 tgttgggttc ataaaacatt aatttcggtt tcgggtgact ttaatcacgt ctggcatcac
    14581 gcggattttg cgcatgatat tcgccagatg cacacggtca cgggcggtca gacgaataaa
    14641 ggcgctatag acgcgaccat ctttctcttc cgtattcaaa ctttgaatat tcgaggtcgt
    14701 ggtgttaatt gccgccgtca ggtttgccag cgcgccctga tgattgaaca tctccacctt
    14761 gatttcggtg atgaattcct gcgccgtctc tttatcccat tcaacagcca taaacttctc
    14821 tggctctttc tggtagccac ggatgttacg gcaggattca tggtggatca ccagaccttt
    14881 accggggctg acgtgggcga taatcgggtc gccaggaatt gggcggcagc atttcgcaaa
    14941 ggtgatcagc acgccatcgg cacctttaat gggcagatgt ccgtgacttt gggttgccgg
    15001 tggaatggag gcgtccccat gttgcagatt tttcgcgacc accacgctca ttgcgttacc
    15061 aaggccgatt tctgccagca gatcgtcaag cgttgccagc ttcatgcggt ccagctcgcg
    15121 ctgaatattt tcctgcggga tttcattgag cttacggcta ccacccaacg catggttgag
    15181 cagacgacgg cccaggctta cagaatcatc acgcttgagg tttttcagca actgacgaat
    15241 tttggcgcgc gctttcgagc tgacgacaaa gttcagccac gcggcattcg ggcgagcacc
    15301 cggtgcggta atgatttcaa cggtttgacc gctagtaagc ggctgcgaca gcgggtaagg
    15361 ctggcggtcg acgcgtgcgc ccacgcaggc atggccgata tcggtatgca ccgcataagc
    15421 gaagtcgacg ggcgttgcac cggcaggcag ttcgacaatg cgcccttctg gtgtgaaaac
    15481 gtaaatctca tccgggaaga gatcggattt aacgctctcg ataaattcaa acgagctacc
    15541 ggcactctgt tgcagctcca gcaggctttg catccagcgc tgggcgcgta tttgcgcggt
    15601 agtgctggtt tcgccgtgct ctttataagc ccagtgcgcg gcaacaccca tctccgccat
    15661 ctgatccata tcttcggtac ggatctggac ctcaaccggc acgccgtgcg ggccaatcat
    15721 cgaggtgtgc aaagactgat agccgttcgc ttttggaatg gcgatatagt ctttcacgcg
    15781 gcccggacgc ggcttgtaca ggctgtgcat ctggcccagc acgcgataac aggtgtcaga
    15841 atcattgacg atcacgcgga aagcgtagat gtccatgatt gagtgaaaac gctgctcttt
    15901 gagcaccatt ttgcagtaaa tcgaataaag atgcttttcg cgaccactga cgcggcacgg
    15961 tattcccgct tcctgcaaac gcccttcgat ttcagaaaga atcttctgga tcatctcttt
    16021 acggttgccg cgcgcggctt tcaccacttc tttgattacg cgataacggt tgggatacag
    16081 cgcctcaaaa cccagctcct cgagttcggt tttaatgtgg tggataccta aacggtgcgc
    16141 cagcgggctg tatatttcga gagtttcacg ggcgatgcgg cgacgtttgt ccgggcgaag
    16201 tgagcccagc gtgcgcatgt tgtgggtacg gtcggcaagt ttgatgagaa tgacgcggat
    16261 atcctgcacc atcgccataa tcatcttgcg aaagttttcg gcctgcgcct ctttcttatc
    16321 gcggaacttg agtttatcaa gtttcgacac cccctctacc agctcggcga cgcttttacc
    16381 aaaaagctgt tccatgtcct ggtaggtggc gggtgtatct tcaatcacat catgcagcag
    16441 cgccgccatc agcgtttcat agtcgagttt catctcggcc agaatgcagg caaccgccac
    16501 cggatgcgtg atatagggtt caccgcttga acgtgtttgc ccctcgtgag catcacgtgc
    16561 aacgagatac gcctgccgca gacgcttgat ttggtcttcc ggcaggtagt tttgaatcag
    16621 ctgattcagg ctttcaaaca gatacaaggg cgacccgctt tgtgattaac gacgaccttc
    16681 agcaatagcg gtaacggctt gtaattcagc ggcttcctgc tcttgctgct cctggcgttc
    16741 gcgaacgtcg aggatctggt tgttgatcag accttcttcg atttcgcgca gcgcgattac
    16801 agtggtttta tcgttttctt ccggtaccag cggatccttt ccgcctacct gcatctgacg
    16861 agcgcgacgc gcggcgacca gtaccaggtc aaaacggtta ccaattttct ctacagcgtc
    16921 ctgaacagtt acgcgtgcca tacttaaaaa gctccacagg tgaagaaatg actgggcatg
    16981 atactgaaat caggttcagt ctgccaacaa tttgctgatt aaagcgtcat gacgctgctt
    17041 ttggcggctc atgcgcagac gttcggcgcg aataatggtc ttcaaatcgg tcaacgcggt
    17101 atcgaagtca tcattcacaa tcaggtaatc atattcggcg taatggctca tttctgcaac
    17161 agcttgcgcc atacgctttg caatgacctc ttcgctgtcc tgaccgcgac cgcgtagacg
    17221 gcggtccagt tcgattttag acggcggtaa aataaagata ctccgcgcgt gcggcatctt
    17281 ctggcgaatt tgctgcgcac cctgccagtc gatatcgaga aaaacatcga caccggtcgc
    17341 cagtacttgc tcaatggctt cacgcgaagt gccatagtaa ttaccaaaaa cttctgcgtg
    17401 ttcgaggaac gcatctctac taatcatttc tttaaattca tcatgattaa caaagaaata
    17461 atgttcaccg tggacttcac caggacgcgg ttggcgtgtg gtgtgtgaaa cagaaacctg
    17521 ggtgtcatac aacggttggg tttttaataa agcctgaatc aggctggatt tacccgcgcc
    17581 actgggggca gaaacaatat aaagcgtgcc ttgagccatg agtatctttc gtatgtgatt
    17641 agcgaaataa agcctacata cgagcttatt atacacggcg cggccatgtg acgtagccct
    17701 tgctcacact ttttgcgcaa agttgttgcg ttttgcgtag ctgcttccgc atttgcctgt
    17761 gattcactgc aataaagacg attctcagga agcgtcctcg cagactccgt ttttgccact
    17821 cggctttgcc ttcaccacat tgtatatctg cctgactatc tacagcggag gtgatgatga
    17881 aagtatggat ggcgatatta ataagtatct tgtgctggca atcatcggtg tgggcggtct
    17941 gtccggcctg gtcgccagcc agagcacagg aagaaatttc ccgcctgcaa cagcaaataa
    18001 aacagtggga cgatgactac tggaaggaag ggaaaagtga ggtggaagac ggtgtttacg
    18061 atcagttaag cgcccgtctt acgcagtggc aacgctgttt tgggaacgag acccgcgatg
    18121 tgatgatacc gcctttaaac ggtgcggtta tgcatccggt tgcccatacg ggtgtacgca
    18181 aaatggcgga taaaaacgca ttaagtcttt ggatgcgcga acgtagcgat ctttgggtgc
    18241 agccaaaagt tgatggcgtt gcggtaaccc tggtttatcg ggacgggaaa ctgaacaaag
    18301 caatcagtcg cggcaatggc ctgaaaggcg aggactggac gcagaaagtt cgcttaattt
    18361 ccgctgtgcc gcaaaccgtt agcgggcctt tagccaacag cacgcttcag ggggaaatat
    18421 ttctcaagcg taaggggcat atccaacaac aaatgggggg aataaatgcc cgcgcaaaag
    18481 ttgctggcct gatgatgcgc caggggaata gcgatacgct aaactcactg gctgtttttg
    18541 tctgggcatg gccggatgga ccgcatttaa tgaccgatcg tttaaaggat ctggctaccg
    18601 caggttttac tttaacgcag acgtataccc gcgcggttaa aaatgctgat gaggttgcgc
    18661 acgtacgcaa tgagtggtgg aaagcaaaat tacccttcgt caccgatggc gtggttgtgc
    18721 gagcggcgaa agaacccgaa tcccgtcatt ggctaccggg ccaggcggag tggctggtgg
    18781 cctggaaata tcaacctgta gctcaggttg ccgaagtgaa ggcgattcag tttgcggtgg
    18841 gtaagagcgg taaaatatcg gtggttgcgt cacttgtacc tgtcatgctg gatgataaaa
    18901 aagtccagcg ggtgaatatt ggttccgtca ggcgctggca ggagtgggat attgcgcctg
    18961 gtgatcagat cctcgtcagc cttgccggtc aggggattcc tcgcattgat gatgtggtct
    19021 ggcgcggagc agaacgtaca aaaccgacac caccagaaaa ccgctttaac tcgttgacct
    19081 gctactttgc ttctgatgtt tgtcaggaac agttcatttc acgcttagtc tggctggggt
    19141 caaaacaggt tctggggctt gatggcattg gtgaggccgg ctggcgcgcc ctgcatcaga
    19201 ctcatcgctt tgagcatatc ttttcctggc ttttattaac gccagagcaa ttacagaaca
    19261 cgccggggat cgcgaaaagt aaaagtgcgc agttatggca tcagtttaat ctggctcgtc
    19321 agcagccttt tactcgctgg gtgatggcaa tgggaatacc gctaactcgg gcggcgctta
    19381 atgccagtga tgaacggtcc tggtcgcaac ttttatttag cacggaacag ttctggcagc
    19441 agctgccggg gactggatcg gggcgcgcca gacaggttat tgaatggaag gaaaatgcgc
    19501 aaatcaagaa gctgggcagt tggctggctg cccagcagat cacaggtttt gaaccttagt
    19561 gaccttcgtg gctgtagtaa aagaccggta atcccagctt aagacgtagc gccagtaaac
    19621 gggcaaaaaa gccgaatacc agggtggaga taatcaccac atcatggtta gaaacatagt
    19681 gttgcagcgc gatgtacagc acggcggagg caaatgagac acccgcatac aactctttct
    19741 ggaataccag tgggatgcgt ttacagaaca tatcgcgcaa aacgccgcca aacacgccgg
    19801 tggttaccgc cgcgacaacg gcaataatcg ggccgtgccc catatccaga gcaacctgtg
    19861 cgccgatgat agaaaatacc accagtccga gcgcatcgag caccagaaac actttgcgca
    19921 gataaggcat tacaggagca actatagtgg tgagtactgc agcggtagcg acgataatca
    19981 catattccgg gtgtttgacc cagccgagcg gatagtggcc cagcagaata tcgcgcactg
    20041 accctccgcc aattgccgtg gcggtcgcaa taataattac gccaaatgta tccatgcgcc
    20101 gtcgtccggc cgccagcgcc cccgtcatgg cttcggcagt aatgccaacc aaatacaaaa
    20161 tgtgtaacag catggttctc tccctaaaaa tctgtctgga agggtagcga tttgtgcgca
    20221 ttgtcacgat tgagattttt taaggagaga atagattgat aatttgtagt tatgtgaaat
    20281 tgctattttc tgtagtaaga atgcattttt ctgattttgt gataagtaaa atgcattaga
    20341 aaaagtatgg agtaaataaa tatgaggacg aaactatatg cgtagcgccg ctgtaattaa
    20401 caacggccta cggagatatg tcgcttactc gatattctga atctgctcgc gcatctgttc
    20461 aatcaacact ttcagctcga tggcggagtt tgtcacttcg gcgttgatag atttcgacgc
    20521 aagagtgttc gactcgcggt tgaactcctg catcataaag tccagacgac gaccaacggc
    20581 ttcttttttc ttcagaatgt tgtaggtctc tttgacatgc gcttcgaggc ggtccagttc
    20641 ttcggcaacg tcaattcgtt gtgccagcag aaccaactcc tgctccagac ggttgttttc
    20701 cagttgcacc tgagcatctt ccagcttcgc taccagacgc tcacgctgcc attgcaggat
    20761 ttccggcata tgggcgcgga ctttgaccac ttcggcggtg acgccttcca gacgctgctc
    20821 aatcaacact ttcagcgcct gaccttcggt ttcgcgcgcg atgataaagt catccagggt
    20881 gccatccaac gccgcgagaa tttcagctgc aatcgcgtcg agatcttgct cctgggctgc
    20941 catcacgccc ggccagcgta gaatatcaac cgggttgatt tctccttcgt cgctctgcat
    21001 ttttacccag ttcgcggcag ttaccagctg tttagccagt ttttcgttga ggatcagctc
    21061 accttgcgcg ctaacatctg gctcatagcg cagagtacat tccactttac cgcgcgtcag
    21121 gcgagaacga atacgctcgc gaacgacagg ttcaaggcta cggaactgct ccggcagacg
    21181 aaagtaagtt tccagataac gctggtttac cgagcgcatt tcccaggttg cgctccccca
    21241 ttcacccttg atttcacgcc gggcgtaggc ggtcatactg cggatcatag acgttcctgt
    21301 ttataaaagg agaggtggaa ggattatagc catcgatgcc ttgtcaggat aggaataacc
    21361 gccggaagtc cgtataatgc gcagccacat ttgtttcaag ccggagattt caatatgcgt
    21421 ccagcaggcc gtagcaataa tcaggtgcgt cccgttaccc tgactcgtaa ctatacaaaa
    21481 catgcagaag gctcggtgct ggtcgaattt ggcgatacca aagtattgtg taccgcctct
    21541 attgaagaag gcgtgccgcg cttcctgaaa ggtcagggcc agggctggat caccgcagag
    21601 tacggtatgc tgccgcgttc tacccatacc cgtaacgcgc gtgaagcggc gaaaggtaag
    21661 cagggtggac gcacaatgga aatccagcgt ctaatcgccc gtgctcttcg cgcggcagta
    21721 gatttgaaag cactgggtga gttcaccatt acgctggact gtgacgtgct tcaggctgat
    21781 ggtggcacgc gtaccgcttc gattacgggt gcctgcgtgg cgctggcaga tgcgctacag
    21841 aagctggtgg aaaacggcaa gctgaaaact aatccaatga aagggatggt agccgcggtc
    21901 tctgtcggga ttgtgaacgg tgaagcggtt tgcgatctgg aatatgttga agactctgcc
    21961 gcagagaccg acatgaacgt agtgatgacc gaagacgggc gtatcattga agtgcagggg
    22021 acggcagaag gcgaaccgtt cacccacgaa gagttactca ccttgttggc gctggcccga
    22081 gggggaatcg aatccattgt agcgacgcag aaggcggcgc tggcaaactg atttttaagg
    22141 cgactgatga gtcgcctttt ttttgtctgt agaaaagtaa gatgaggagc gaaggcatga
    22201 aaccatatca gcgccagttt attgaatttg cgcttagcaa gcaggtgtta aagtttggcg
    22261 agtttacgct gaaatccggg cgcaaaagcc cctatttctt caacgccggg ctgtttaata
    22321 ccgggcgcga tctggcactg ttaggccgtt tttacgctga agcattggtg gattccggca
    22381 ttgagttcga tctgctgttt ggccctgctt acaaagggat cccgattgcc accactaccg
    22441 ccgtggcgct ggcggagcat catgaccttg acctaccgta ctgctttaac cgcaaagagg
    22501 caaaagacca cggtgaaggc ggtaatctgg ttggtagcgc gttacaagga cgcgtaatgc
    22561 tggtagatga tgtgatcacc gccggaacgg cgattcgcga gtcgatggaa attattcagg
    22621 ccaatggcgc gacgcttgca ggcgtgttga tttcgcttga tcgtcaggaa cgcgggcgcg
    22681 gcgaaatttc ggcgattcag gaagttgagc gtgattacaa ctgcaaggtg atctctatca
    22741 tcaccctgaa agacctgatt gcttacctgg aagagaagcc agaaatggcg gaacatctgg
    22801 cggcggttaa ggcctatcgc gaagagtttg gcgtttaacg aaactcgccg gatgaaaagt
    22861 catccggcgt catattactg caactgtgcg gcaattagcg gccagcgggc gtcaaaatca
    22921 tccgtcgggc ggtatttaaa ctcgctgcgg acaaatcgtg acagcatacc ttcacaaaat
    22981 gccagcaact ggcttgccag cagggtttca tcggtggtgt aaccttcacc ctcacgcatt
    23041 ctcttttcac gcaatacctg acgcaactgc gcttcaatac gctcgaacag ctggttgatg
    23101 cgcccttgca gacgatcctg ttcgaacatt agcgcatgac cagtgaggat gcgagtcagg
    23161 ccaggattac gctcaccaaa accgaggagc aacaacacga tcagacgcag gcgcgctgtg
    23221 gtgtctttct catctttcag aatcaggttg atacgagtaa tcaggctatc ttcgataaac
    23281 tcaatcaggc tatcgaacat gcgggtctta ctggggaagt ggcgatacag tgccgcttcg
    23341 gaaacgccga cagaggcggc cagttttgcc gtcgtgatac gttggcttcc atcgctggat
    23401 tccagcatca gcgccagaga ctgaagtatt tcctcgcgac ggttcctttt cgcagtttgt
    23461 ttttctgcca tgttacaaaa tacccctgaa aataagcact tgccaggcgg cacccacgct
    23521 atgaccgcaa acgaaatgtt tgcggctatg ttatgacgtt attcggatgc gtatgtgtta
    23581 ctgacgacca gagtgaccaa agccgccttc accgcggtcg gtggcgtcga aatcttccac
    23641 cagattaaat tcagcctgta ctaccggaac aaaaatcatc tgggcgatgc gttcgccagg
    23701 ttgaatggtg aagctgtcct gaccacggtt ccacacggaa atcatcaact ggccctgata
    23761 gtcagaatcg atcaatccta ccaggttacc aagcacgata ccgtgcttat gtcccaatcc
    23821 ggagcgcggt agcatcattg ccgccagtga aggatcggca atatgaatcg ccagcccggt
    23881 cggaaccagc gtagtgtcac ccggagccag ttctacggcg tcgtcgagac aggcacgcag
    23941 gtcaagtccg gcagagccag aggtggcata agtcgggagc ggaaattcct tcccaacgcg
    24001 cgggtccaga atcttaacgt cgattttttt catcataacg ggtcacgatc tcgtcgagta
    24061 ataattggcc aaggagctct ttgcgctcaa gcggtaagac tttatctccg tcctgccaga
    24121 aaaggtgtaa tgcgttgttg tcgctgttaa atccttgagt tggctgggaa acatcgttcg
    24181 cgcagatcag atcaaggttt ttacggatac gtttttgccg ggcgtattct tccacattat
    24241 ttgtttcggc ggcaaatcca acgacgtaag gtcgatggtc ttttagtgcg gcaacgcctg
    24301 cgacgatatc ggggttttta accattttta ttgttaattc atcaccctgc gtggcctgct
    24361 ttttgatttt ttctggggcc acggtagctg cgcgataatc cgccacggcg gcgcaaccga
    24421 taaaaatatt ttgctgctgt actgaagcat tcacggcggc ttccatttcc agcgcggtca
    24481 tcacatcaac acggttaaca aacggtggcg tcggtagtga aaccggacct gataccagcg
    24541 tgacgttcgc gccacgacgg gctgcggcgg cggcgatagc aaaacccatc ttgccggagc
    24601 tgtgattaga gatataacgc acgggatcga gcggttcacg cgtcggaccg gcggtaatca
    24661 taatgttcag atgtttcagg tcgttgacgg gcgaaaaatg cgctaccgcc atatccacaa
    24721 tagttaacgg atcgagcatt cgtcctgggc cgatatcacc acaagcctga ctgccgctgt
    24781 ctggccccca gatgagcaaa ccacgtgagg caagcacctc taaattatgc tgggtggcgg
    24841 cggcacggta catctgctgg ttcatggcgg ggagcacggc tacaggcgca ggtgtagcca
    24901 gacaaatcgt cgataccagg tcattcgcca ttccggctgc aacacgggca attaaatctg
    24961 ccgtggcagg ggcgagaatc actaaatcag cccattttcc cagctcaata tggcccatag
    25021 cggcttcggc tgccgggtcc agcagactgt cggaaacggg ataaccagaa accgcctgca
    25081 agctaagtgg ggtgataaag gcttttgccg cttcggtcat ggctacgcag acatcggccc
    25141 cgcgatcgcg caaacgacgc accagttcag gggttttata ggcagcaata ccgccgctaa
    25201 cgccgagaac gattttttta ccggccaggc tcatgatgat ttttcctgtt gggtgacacc
    25261 agaagttggc gattttatca caatactttt gttgtcgtgc cttcacctga gattcacttt
    25321 gcgaggcgct ttccaggatt gaaaactggc cgtcgattta aaggaacggc tatgacagga
    25381 tgcgagcacc acaaaggagg tgaaggtgaa aaacaatgta cagctgttga tgccgcgcga
    25441 aaaaatgctg aagtttggta ttagcgcctt aacggatgtc gagctgctgg cgctatttct
    25501 gcgtaccgga acgcgcggta aagatgtatt aaccctggca aaagagatgc tggagaattt
    25561 cggctctctt tatggcttgt taacctctga atatgagcaa tttagtggcg ttcatggaat
    25621 tggcgtggcg aaattcgccc agttaaaggg gattgccgaa ctggcgcggc gttactacaa
    25681 cgtacggatg cgtgaagaaa gccctttact cagcccggag atgacgaggg aatttttaca
    25741 aagccagctc acgggcgagg agcgggagat ctttatggtg atctttctcg actcccaaca
    25801 cagggttata acgcatagcc gtcttttttc cggcacgcta aaccatgttg aagtccatcc
    25861 tcgggaaatt atccgcgaag cgataaaaat aaacgcctcg gcgctgatcc ttgcgcataa
    25921 tcacccttcg ggttgtgctg aacccagtaa agcggataaa ctcattactg aacggataat
    25981 aaagagttgt cagttcatgg atttacgcgt gctcgaccat atcgtgattg ggcgtggaga
    26041 gtatgtttct tttgccgaac gcggctggat ttaacccgct atgcgcgatc cttcgggatc
    26101 tttgtctgtt cgggacttga gcacatcgct gagtcagcgt atactacgcc acctttgaga
    26161 atctcgggtt tggcatttgg gcctggcaat cgagagttca catagaactg cgatgaccgg
    26221 gctgtaaagc ctgacgaggc gccaataccc catacgaagc tcgagctaat ttgatttttg
    26281 gagaatagac atgtcccgag tctgccaagt tactggcaag cgtccggtga ccggtaacaa
    26341 ccgttcccac gcactgaacg cgactaaacg ccgtttcctg ccgaacctgc actctcaccg
    26401 tttctgggtt gagagcgaga agcgttttgt caccctgcgc gtatctgcta aaggtatgcg
    26461 tgtaatcgat aaaaaaggca tcgatacagt tctggctgaa ctgcgtgccc gtggcgaaaa
    26521 gtactaagta cttagaggaa ataaatcatg gctaaaggta ttcgtgagaa aatcaagctg
    26581 gtttcttctg ctggtactgg tcacttctat accactacga agaacaaacg tactaagccg
    26641 gaaaaactgg aactgaaaaa attcgatcca gttgttcgcc agcacgtgat ctacaaagaa
    26701 gcgaaaatca aataatttcc gctttaatgt aacaaaaaac ctcgccccgg cggggttttt
    26761 tgttatctgc ttgcccccat attgactgca tctgttcatt cctggagatg ctatgcctga
    26821 attacccgaa gttgaaacca gccgtcgcgg catagaaccg catctcgttg gtgcaaccat
    26881 tcttcatgcg gtggtgcgca acggacgctt gcgctggccg gtttcagaag agatctaccg
    26941 tttaagcgac caaccagtgc ttagcgtgca gcgccgggct aaatatctgc tgctggagct
    27001 gcctgagggc tggattatca ttcatttggg gatgtctggc agcctgcgca tccttccaga
    27061 agaacttccc cctgaaaagc atgaccatgt ggatttggtg atgagcaacg gcaaagtgct
    27121 gcgctacacc gatccgcgcc gctttggtgc ctggctatgg accaaagagc tggaagggca
    27181 caacgtgctg gcccatcttg gaccggagcc gcttagtgac gatttcaacg gtgagtacct
    27241 gcatcagaag tgcgcgaaga aaaaaacggc gattaaaccg tggctgatgg ataacaagct
    27301 ggtggtaggc gtagggaata tttatgccag cgaatcgctg tttgcggcgg ggatccatcc
    27361 ggatcggctg gcgtcatcac tgtcgctggc ggagtgtgaa ttgttagctc gggtgattaa
    27421 agcggtgttg ctgcgttcga ttgagcaggg cggtacaacg ctgaaagatt ttctgcaaag
    27481 tgatggtaaa ccgggctatt tcgctcagga attgcaggtt tacgggcgaa aaggtgagcc
    27541 gtgtcgggtg tgcggtacgc cgattgtggc gactaaacat gcgcagcggg caacgtttta
    27601 ttgtcggcag tgccagaagt aattgatgcg cgccggatgg cataccatcc ggcataaact
    27661 ctacgctaac ttcgccatca gcgcctgatg gacattctcc ggcaggaaat gggtgacatc
    27721 gccctgatgg cgcgccacct ctttcaccaa cgatgaagag ataaacgacc actctttcga
    27781 cggcatcaga aacacacttt ccagctccgg cattaagtgg cgattcatat gcgccagctg
    27841 catttcatat tcaaaatccg ccaccgcacg caggccacga atcagcaccg tagcgtgttg
    27901 attacgggcg aagttcgcca tcaaatcact aaacccgacc acttccacgt tctccagatg
    27961 cgcggttgcc tgctgtgcca gctccacacg ctcttccagg gtaaacatcg gttttttact
    28021 gggactagcg gcaatcgcca gaataacgtg atcgaacatt tgcgaggcgc gcgtcacgat
    28081 atcgatatga ccattggtaa tgggatcgaa agtacccgga taaatcgccc gtttttgcat
    28141 aacaacctca atgcgttttc ggtggcaggt aaggttccag cagttgaagc agacgctgta
    28201 gtgcgccctg gttttgatac agcacttcaa cggcatgacg gccatagaaa ctacggtaat
    28261 cggcgtcggt gagtaaagag gaaacctctt ttgcaagcgt agtggcatcg gtaacggtaa
    28321 tcagcccgct tgcctgctcc agccgcgcgc aaatgtcttt aaagttaaac gtatgcggcc
    28381 ccatcaatac cggaatagcg tgtgcggccg cttccagcgg attatgccca ccacgttcaa
    28441 ccagtgaacc gccaacaaag gcgagatcgg caatgccgta cagtaacatc aactcgccca
    28501 tcgtatcgcc aaccacaacc tgcgtgctgg tggaggggac ttcccctgaa gatcgtgtga
    28561 tatagcttag tccagcctgg cggacaaggt taatcgcatc cgggaagcgt tctggatgac
    28621 ggggtaccag gatgagcaat aaattcggga attgctgtaa caatgcctga tgtgcggcga
    28681 tcaccacact ctcttcgcct tcatgagtgc tggtggcaat ccataccggg cggtgtggtg
    28741 cccactggcg gcgcagcgtc acggctttag cagccaactg cggcgttaca gaaatatcga
    28801 atttcaggct accggtaacg gtcacctgat tattttttgc gcccagcgcc acaaaacgtg
    28861 caccatcttc ttcattttgg gcggcaatca gcgtaatacg acgcagcaag cggcggacga
    28921 atttacccag tttggcataa cctgcggccg agcgggcaga gagtcgcgcg ttagcgatca
    28981 ccagcggaat tttacgttta tgtagcgccg caatcaggtt aggccatagt tcggtttcca
    29041 taatcaacac cagtttaggg tcgactttat tcaggaaacg gttgagtgca tcgggcaggt
    29101 catacggcag ataaacgtgc tgaacatcct tcccgaaagc cgattgtacg cgctccgaac
    29161 cggttggagt catggttgtt acggtaatcg gtaaatcagg ataacgatga cgcagtgcgc
    29221 gcaccaacgg gatcgccgcc agagtttcac cgacggagac ggagtgcagc ataatgccgc
    29281 ctggttttag cggatggcgg taaaaaccgt aacgttcacc ccagcgtttt cgataggccg
    29341 gagccttacg tccgcgcacc cagagccgta tccagatcag tggctgaata aggtagagaa
    29401 gggcggtgta aagcaattcg agcatagtaa atagctgact tatggatgtg ctggggattc
    29461 tatgtattta gctgtggctt taccattact tttcccgttt ttgacttaaa tagcaattta
    29521 cccgccaatt cttgctgaac atctatactt tttttgtatt tttatgcgat tcattgattt
    29581 ctgcagtacg tgcaggaagg aatcgcagag caacagtaaa aagaatatgg ttgctaatga
    29641 ttttatttga ttgatttctt tattgaaaat caacaatgtg cttaaaaatc attcgggtcg
    29701 gattgccgcg aaagaagtga tacactagcg cgtcaatatc agtgcggtat cagtattcag
    29761 gtagctgttg agcctggggc ggtagcgtgc tttttctgct tcaccttaat cggataatct
    29821 caacaaaaag agttacttgt ggataagcca tttcgaagaa ttttgctcat taagatgcgt
    29881 tttcatgggg atatgttatt aactactccc gtcattagtt cgctgaaaaa aaattaccct
    29941 gacgcaaaaa tcgatgtgct gctttatcag gacaccatcc cgatcctgtc tgaaaatcca
    30001 gagattaacg cgctctacgg cataaaaaat aaaaaagcaa aagcctcaga aaaaattgcc
    30061 aacttttttc atctcatcaa ggtattacgt gccaataagt atgaccttat cgttaatctt
    30121 accgatcaat ggatggttgc tatactggtt cgcttattaa atgcccgtgt gaaaatttcc
    30181 caggattatc atcatcggca gtctgctttt tggcgtaata gtttcactca tttggtgccg
    30241 ttgcagggtg gaaatgtggt ggaaagtaac ttatccgtgc tgacgccatt gggacttgaa
    30301 tcgttggtga agcagacaac catgagttac ccgcctgcaa actggaaacg tatgcgtcgc
    30361 gaacttgatc acgctggtgt tggacaaaat tatgtggtta tccaacctac ggcgcggcaa
    30421 atcttcaaat gctgggacaa cgccaagttt tccgctgtga ttgatgcctt acatgctcgt
    30481 ggttatgaag tcgttctgac gtccggccca gataaagacg atctggcctg cgtcaatgaa
    30541 attgcgcagg gatgccagac gccaccagta acggcgctgg ccggaaaggt gaccttcccg
    30601 gaacttggtg cgttaatcga tcatgcgcag ctgtttattg gcgttgattc cgcaccggcg
    30661 catattgccg ctgcagttaa tacgccgctg atatcgctgt ttggtgcgac ggaccatatt
    30721 ttctggcgtc cctggtcaaa taacatgatt caattctggg cgggagatta ccgggaaatg
    30781 ccaacgcgcg atcagcgtga ccgaaatgag atgtatcttt cggttattcc ggcggcagat
    30841 gtcattgcag ctgtcgataa attacttccc tcctccacga caggtacgtc gttatgatcg
    30901 ttgctttttg tttatataaa tattttccct ttggcggttt gcagcgcgat tttatgcgta
    30961 ttgctcagac agtcgccgcc cgaggccatc atgttcgggt ttatacccag tcgtgggaag
    31021 gcgaatgccc tgatgtattt gaactgatca aagtgccggt taaatcgcat accaatcacg
    31081 ggcgcaatgc ggaatatttt gcctgggtgc aaaaacattt acgtgaacac cccgtcgata
    31141 gagttgttgg atttaataaa atgccggggc tggacgttta ttatgccgct gatgtttgtt
    31201 atgccgagaa agtagcgcag gagaaaggct ttttctatcg cctgacgtca cgttatcgcc
    31261 attatgctgc ctttgagcgg gcaaccttcg aacagggcaa gccgacacag ctgctgatgc
    31321 tgacagataa gcaaatcgcc gatttccaga aacattatca gactgaagcg gaacgttttc
    31381 atattctgcc accggggatt tatcctgatc gtaaatatag ccagcagcca gccaatagcc
    31441 gtgaaatctt ccgtaagaag aatggaataa ccgaacaaca atatttattg ttgcaggttg
    31501 gttcagactt cacgcgtaaa ggtgtcgatc gttccattga agcacttgct tcgttaccgg
    31561 attcgctgcg ccacaacaca ttgctatatg ttgttgggca ggataaaccg cgaaaatttg
    31621 aggcgctggc agaaaaacgc ggcgtgcgca gtaatgttca cttcttctcg gggcgcaacg
    31681 atgtctcgga attaatggcg gcggcggatt tattacttca tcctgcctac caggaagccg
    31741 cgggaattgt actgctggaa gcgataactg caggattacc ggtactaaca acagccgttt
    31801 gtggctatgc gcattatatt gttgacgcca attgcggcga ggctattgct gagccattcc
    31861 gccaggaaac attgaatgag attttacgca aagcgttaac gcaatcttca ttgcgccagg
    31921 cctgggcgga aaatgcgcga cattatgctg atacacaaga tttatacagt ctgccagaga
    31981 aagcggcgga catcataacg ggtggtctgg atggttgaac ttaaagagcc gtttgccacg
    32041 ttatggcgcg gtaaagatcc ttttgaggaa gttaaaacct tgcagggtga ggtatttcgt
    32101 gaactggaaa ctcgccgcac tctgcgcttt gaaatggcgg gcaaaagcta ttttctcaaa
    32161 tggcatcgcg gcacgaccct gaaagagata atcaaaaatt tactctcatt gcggatgcca
    32221 gtattaggcg cagaccgcga atggaatgcg attcatcgac tgcgggatgt cggcgttgat
    32281 actatgtatg gggtggcatt cggcgaaaaa ggcattaatc cgctgaccag aacctcgttt
    32341 attattaccg aagatctgac accaaccatc agtctggaag attactgtgc tgactgggcg
    32401 accaacccac cagatgttcg cgtgaagcgt atgcttatta agcgtgtcgc gacgatggtg
    32461 cgcgatatgc atgctgcggg cattaaccac cgtgactgtt atatctgtca tttcctgctg
    32521 cacttgcctt tttccggtaa ggaagaggag ttaaaaattt cagtaattga cctgcaccgg
    32581 gcgcagatac gtgcaaaagt accgcgtcgt tggcgggata aagatttgat tggtttgtat
    32641 ttttcatcaa tgaatattgg gctgacgcag agagatatct ggcgatttat gaaggtttat
    32701 tttgggatgc ctttacgtaa gatattatct tttgaacgga atttattga
//
LOCUS       DACADF010000042        32315 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.042, whole genome shotgun sequence.
ACCESSION   DACADF010000042 DACADF010000000
VERSION     DACADF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.042"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(575..664)
                     /locus_tag="ISB30_001864"
     tRNA            complement(575..664)
                     /locus_tag="ISB30_001864"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(628..630),aa:Ser,seq:cga)
     gene            758..1555
                     /gene="mtfA"
                     /locus_tag="ISB30_001865"
     CDS             758..1555
                     /gene="mtfA"
                     /locus_tag="ISB30_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAP1989502.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEILIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1656..1731
                     /locus_tag="ISB30_001866"
     tRNA            1656..1731
                     /locus_tag="ISB30_001866"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1689..1691,aa:Asn,seq:gtt)
     gene            1893..3155
                     /locus_tag="ISB30_001867"
     CDS             1893..3155
                     /locus_tag="ISB30_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1989503.1"
                     /translation="MSLTSAKIRTLKPSDKPFKVSDSHGLYLRVKPGGSRHWYLKYRI
                     SGKESRIALGTYPAISLSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(3349..4653)
                     /gene="ybtS"
                     /locus_tag="ISB30_001868"
     CDS             complement(3349..4653)
                     /gene="ybtS"
                     /locus_tag="ISB30_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HAP1989504.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(4681..5961)
                     /gene="ybtX"
                     /locus_tag="ISB30_001869"
     CDS             complement(4681..5961)
                     /gene="ybtX"
                     /locus_tag="ISB30_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HAP1989505.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(5954..7756)
                     /gene="ybtQ"
                     /locus_tag="ISB30_001870"
     CDS             complement(5954..7756)
                     /gene="ybtQ"
                     /locus_tag="ISB30_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HAP1989506.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWGHE"
     gene            complement(7743..9455)
                     /gene="ybtP"
                     /locus_tag="ISB30_001871"
     CDS             complement(7743..9455)
                     /gene="ybtP"
                     /locus_tag="ISB30_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HAP1989507.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVVRLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVME
                     NERQ"
     gene            9712..10671
                     /gene="ybtA"
                     /locus_tag="ISB30_001872"
     CDS             9712..10671
                     /gene="ybtA"
                     /locus_tag="ISB30_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HAP1989508.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQHRCNAETHLTA
                     QALRHALMRMHSGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFTPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            10862..16969
                     /gene="irp2"
                     /locus_tag="ISB30_001873"
     CDS             10862..16969
                     /gene="irp2"
                     /locus_tag="ISB30_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HAP1989509.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNCYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDTYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     VVRDGQQQVLEQTPRWVIPAHILHTPEEALQVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            17057..26548
                     /gene="irp1"
                     /locus_tag="ISB30_001874"
     CDS             17057..26548
                     /gene="irp1"
                     /locus_tag="ISB30_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HAP1989510.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLDALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLTPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIARHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAVVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPAHWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            26545..27645
                     /gene="ybtU"
                     /locus_tag="ISB30_001875"
     CDS             26545..27645
                     /gene="ybtU"
                     /locus_tag="ISB30_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HAP1989511.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPQVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            27708..28445
                     /gene="ybtT"
                     /locus_tag="ISB30_001876"
     CDS             27708..28445
                     /gene="ybtT"
                     /locus_tag="ISB30_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HAP1989512.1"
                     /translation="MCPFAGGSSSAFRHWQAEQLADCALSLVTWPGRDRLRHLEPLRN
                     ITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFETCRLLEQRGLAPQGLIISGCHA
                     PHLHSERQLSHRDDADFIAELIDIGGCSPELRENQELMSLFLPLLRADFYATESYHYD
                     SPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLSHVTGPVVIDGDHFYPIQQARSF
                     FTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            28449..30026
                     /gene="ybtE"
                     /locus_tag="ISB30_001877"
     CDS             28449..30026
                     /gene="ybtE"
                     /locus_tag="ISB30_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HAP1989513.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENLAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            30157..32178
                     /gene="fyuA"
                     /locus_tag="ISB30_001878"
     CDS             30157..32178
                     /gene="fyuA"
                     /locus_tag="ISB30_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HAP1989514.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         6975 a         9686 c         8927 g         6727 t
ORIGIN      
        1 ctcatacttg agtcgtgtct tttttattat gtctgtagaa aaaaactgag aaaaaggcga
       61 acaataaata cacaactcag ccatacagac tggcagcgca aatgtctcca ggagcaagtc
      121 atctgtcatg gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta
      181 cttgttagta tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat
      241 tatggacttt gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt
      301 gaccccttct ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc
      361 aatgtctaaa agaataatgg tgaccgataa gaaaacgact gaataactgt agattttcgc
      421 tcgaaacctt cctgtcagac ccatagcgaa tcaagtgctg aatgtcacag tatcgaacag
      481 aaaacagtga cgatctaacc cttcaagaat atcctacgat tgttctgttt aggaaaagca
      541 aggcgggaag tcgggagata agtcattgat aaagtggcgg agagaggggg atttgaaccc
      601 ccggtagagt tgcccctact ccggttttcg agaccggtcc gttcagccgc tccggcatct
      661 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
      721 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
      781 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
      841 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
      901 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
      961 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     1021 tgaaatctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     1081 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     1141 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     1201 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     1261 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     1321 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     1381 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     1441 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     1501 gagactgcat cacgctaatg atacagactc gttttcggcg acgaatgttc attaattaac
     1561 aactttgcag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     1621 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     1681 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     1741 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     1801 ttctcagtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctgggg
     1861 tcaggttagt tcgataatgg agtgaccccc atatgtccct taccagcgca aaaatccgca
     1921 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgcggg
     1981 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     2041 gcattgcgct gggtacctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     2101 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgtacgtg
     2161 gctcacgaac accggagaaa gtttttaaaa acgtggcgct ggcgtggcat aaaagtaaca
     2221 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     2281 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     2341 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     2401 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     2461 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     2521 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     2581 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     2641 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     2701 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     2761 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     2821 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     2881 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     2941 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     3001 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     3061 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     3121 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     3181 caaattacca ccacctccac aagaggtggt tctatataga cttttatcaa cttcatgacg
     3241 aaaaacacct attttatccc aaaagaagac aatagagtca cagaggaatc aaagagcgac
     3301 tcaattgtct ttggacagtc tggttgtgag gttagaaaac agttactcct acaccattaa
     3361 atagggcgca atgctcgcta atttctcccg ggtttctgtc agttcgcgtt ccggcgtaga
     3421 ttgcgcgatg attcccgccc ccgcctgtat ccagcagcgc tggctatcct gaaatacgga
     3481 acgcagaact agcgccgcat caaagcgcgt atcgtccagc agcaggattg cgccggaata
     3541 aagctctcgc ggcgtcttct caatttgcat aatcgcgttc agagcagcat ttttagggat
     3601 acctgaggcg gtaatcgacg gaaacagcac ggtaaacgca tcccaggcat ccttgttttc
     3661 cgcaagctga ccgctcacgc cagaccccag atgctgaacg ctgccgcgct ggcgaaccga
     3721 cattaaatct tcaaccacca cgctgcccgg ctggcaaacg gcctccagtt cagcaatagc
     3781 ttctttgaca gaaaggatat gctcaagcac ctctttactg tcgtgcagca gttctgcctc
     3841 tttcgcctta ttatgctccg ggtttcccat gcgatcgcgg gtgcccgcaa gcggttcagt
     3901 gaccacttta ttgcccgtca ctgacatcac cagttctggg ctaaagccca gcgcttcgcg
     3961 tccttcctga cggaacataa acgagcgcac aggcgtgttt gcctgccgcc cgtataacag
     4021 cgtggcgggc atatcaatcc gcgatggcag gggaatggcg cgcgagacaa tcactttgac
     4081 atactcgcca cggcggatct ccgcaacggc gcgcgcaacc tgttgtttat atgcctcacc
     4141 attgagggcg gtatccaccg ccagtggtgc gttctgcgtc gttgtaccgg cctcttttac
     4201 ccactcgcac aaacgtcggc acccgtcggc ggagtccgca taaacggtga catttccctt
     4261 ttcaaaaata agttcttcac ggggaacggt taacgtcagc agcggccact ccccggcgtt
     4321 aaaggcaatc ccccgcgcat gcgccgcaaa gttgaaccca acctgtccat atacccgacg
     4381 tcctttcacc tgaggatgcg ccataaaacg acgcgcgcag tcggcaatgg aatccaccgc
     4441 ccatttttgc tccccggcat catcaataaa tgtcgcctgc gttccgtcgg cattaatgtg
     4501 cagccgtgcc tggcaccctt tgcctaaata ccaacagggt tgacgctcat agacataaca
     4561 ctcttcttct gcgaactggc gtaaaacgcc agaaatcgtc ggtagccatt gttcctctgg
     4621 taacgccaga tgtagaaatt cactgatttt catcccttac ctctctgtgt tattcgcgga
     4681 tcaatcaatt gcgcttgtca tcagttctct tgccgaatgc agccggatat aagcaaccca
     4741 cgccgccagc aacgtgaatg ccccggcaaa caggaagcag gccgcatagc caaaatgttg
     4801 agcaacaacg ccaccgataa cgcctgccag aatggcgatt gccgcatcag tacattgaaa
     4861 gagggtgaag tcgacaccgg cctgcaaagg cgatgaaagc gacatcagcg tggcgtaaag
     4921 acagaccagc gcgcaggcca gcgaaatgga ctggacaatg accagacact ggagaatctg
     4981 cagcagcaga tgacccggcg ccatcatgag cgtcatcacc accgccagca gcgcgatccc
     5041 ctggacgcca taagccgtca acagcgctct gccgggtgag gtgtatttca tcagcaatcc
     5101 gccggccagc gttcctgcta tgcccgctgc aatattgccg ccgctgaaca gcgcgcccaa
     5161 tgcggacatg ctcaacccat gatccaacaa cagcggtgcc agaagaggca gcacaaaccg
     5221 catgcctgaa ttcagcatca ataccagcag taagcccagg cgcgcctgct tcctccttag
     5281 cgcataacct aacgccggaa catgcgggat agtcgctgtt cgcgtcggct ccgtaatgcg
     5341 ccacagcggg agtgacagcg ccataatcag caccgccatc atcagcatgg cgacaggcca
     5401 gccggatgcc gccgacagca tcatgaacac cccgccgccg cacatcattc ccagatagct
     5461 gccgccgacc tgcacgctgt ttccccagcc gtaacccgtg cgggtcagtt gatcgacaca
     5521 aaagccgtcg ctggcgatat caatggtgga ggcgaccgtg cctgccacca taaacagaac
     5581 aaataacgcg acgatttgcg tatcggggaa tcccccttct cgcccaaacc agccaatcgc
     5641 tgcggcaata gtcaggatcg ccgctagcgc gacctgacca cgcaggatta acatgcggga
     5701 acggcgttcc tggctaccgg gcggaagccg ccagcgctcg atccacggcg cccaaataaa
     5761 cttcagcgcc cagggcagca taaacagggt tgtcgcaccg gcaagcgcca gcgaaccgcc
     5821 agcagcgcgc accagcgcgg gtaaggactg catagacagc gccgatacca gactctgcgt
     5881 cacatagacg ccggcaatag caaaaatcac ccgcccggtc gtcaacgtca gcggtttcac
     5941 attcgactga acatcactca tgcccccact ctccagacgc ggaaaccccg tcgtcgcgcc
     6001 acacgcgcgc ggccatttgc gcctgccaca gcgcctgata acgtccatga tgtgagagca
     6061 attgcgcatg agtaccctgc tcaaccacct gtccctcttc catcaccaga atgtttccgg
     6121 ccccggcgat ggtggataaa cggtgagcga tgataatcac cgtccggttg taaaccaggt
     6181 tatcgatcgc tttttgcacc gccagctcgc tttcaatatc cagcgcggca gtcggttcat
     6241 cgagaataac gaccggcgcg tttttcaata acgctctggc aatggaaatc cgctggcgct
     6301 cgccgcccga tagctggccg cccatctctc ccattggcgt cagccagcct tgcggaagac
     6361 gggaaataaa ctcaaggcac tgcgccgcgc gggcggcctc ttctacctcc tgccgcgtcg
     6421 cctgtgggcg cgcgatacgg atattcgcca gcagcgtgtc atcaaacagc cagacatcct
     6481 ggaaaacaac agaaatcagg ctattgagct gttccggcgt caggcggcga atatcgacgc
     6541 cgccaataga aatctgccct tgctgcggat cggcatagcg cattaacagt ttggtgacgg
     6601 tagttttgcc tgcgccggag gcacccacca gcgcgctcat actggctgcc gggaacgtca
     6661 aagaaacatg attaagcgcg tggccgtcac cttcttcgta gcgatagctg acgttgtcaa
     6721 agcggatatc gtaacgttcc ggcatctcgc tttgctctgc gacgggaagc ggtgctatcg
     6781 ccataaactg ctcaatcctt tgcagggcgc tggcgatcag ttccacaacc gaggtgtagc
     6841 tgataaacat cgccatcggt tctgcgaagc gcataatcat cgcgacggcg gcaatcaaaa
     6901 aggcgaggtt cagagtgccc gtcactaccc agacgatccc ggataacacc accacctgta
     6961 ggcccaactc cacgacgctg gcgatcagca tcgtcgcgcc agcgccctga cggtgagtgc
     7021 gggtctgtaa gttttccagc gcattgaaat gcgccagcag cgcccggctt ttatcggcat
     7081 cgctgccgca ggtgcgcaat accatcatcc cctgagcaaa ttcaacgata tcgccgctca
     7141 gacgctggtg cgcttccccc agcgtctgca tttgtcgccg catcgccggg cggcgccagt
     7201 aataaaacgg caccagcaga gggaagatca gcaacatcac cagccccagc cgccagtcta
     7261 tccacaatgt cgccagcgac gccgtcagcg gcgtgacaat ggtgagcagc aaaatattgg
     7321 ctatcgcaat aacataattg aggttttcat ccacgctgcc cagcagcaag gcgttcattt
     7381 cacccgccct gccgcgctgg agcttctcca gcggcacgcg gcgtaactgc tcgccaagtc
     7441 gcaggcgcaa ctcatgggta gcctgcgcca gatgaccacg gtattcaaag cccaggccat
     7501 accagcgtag caccagcgtc acaattgcgg cgacgctgaa ggccatagcc cagttaagaa
     7561 gttgcggcgc gtctccccgt aacagcgcat caatgatcgg ataaagacag gcaaaggcga
     7621 tcccctgcat gaatgccgcc agcaacagcg ccagcatact gcgtcgcagc atcctggcct
     7681 gactgccaac gctggagata agctggcgcc agatgacgcg ccaggccagg ttatccgcag
     7741 gattattgtc tttcattttc catcacctct tcctgtgttc cacccggcac ccaatgctgc
     7801 gcctgctgat agtgttgcca gagccgttga tacaggccgc cctgcgccaa cagttgcgta
     7861 tggttcccca tttccctgag ctgtccgtcg gaaaacagca atatcacatc ggcctgagtc
     7921 accattgaga gacgatgcgc gaccatgatg accgtccggc cacgcatggc cgccgcgagc
     7981 gccttgataa gcgccgcttc gttttccggg tcagcaaacg ccgtcgcctc atcgagcacc
     8041 aggatggggc gatcctgcaa aagcgcccgg gcgatagtaa tgcgctgccg ctggccgccg
     8101 gagagaaata ccccacgttc cccgactcga gtgttgtaac cttctggcag agcgctaata
     8161 aaatcatggg cctgcgccac tctggccgcc gctataaccg cctccagcgg cgtatccggc
     8221 gcgcccagac gaatgttatt ggctatcgtg tcggcaaaaa ggaagttgtc ctgaaacaca
     8281 aacgagagtt gcttcatcag ggtgtccgtc tgcatatcac gcagatccac gccgccaata
     8341 cggatatggc ctttgtccgg gtcggcgtaa cgcagcagca agcgcaccac ggtgcttttt
     8401 ccggcgccgc ttggcccgac cagcgccaca atttgcccgg caggcacatg aaagctcacc
     8461 tcctgcaacg cggcgccagt acgcgcttgc ggataatgaa agctcacctg ctcaaaggta
     8521 atgctcgcct cttgcggctg ctggtcagac tgcggcagcg ataactccgg catcgcgaga
     8581 acctgataaa tacgctgaat gcttaaacgc gtttgcgcga ccaggttatt gagcatcatc
     8641 attggcatta cggcttcggc cattccgctg cccagcagta aaacggccac ccacgcgata
     8701 aaatcgaaac tgccatagtg caacaggccg tatcccgacc agatcaggac aaacagggtc
     8761 gggagaggat tcagaatcga gaaggaaaaa cgcgctgaaa aaccggcttt gcgataccag
     8821 gttttgagca catcgaccca ctcttcaagg gcgcgttgat agcgcaaaaa actggtgctg
     8881 ccgctatcaa acgttctcac cacgggcatg gcctgcacga actcaataac cgccgcgcta
     8941 acctgctcgc gcgccttatg ataacgctga gccatatctt ccgaacgctg gcgggcgagc
     9001 acgagaacaa ccgatccaaa cgccagtacc cccagcgtcg cgattgccag ccgccagtcc
     9061 agccagaaca agataacgat tgtcgccagc ggcatgatga tcgcgcgggc atagagcggc
     9121 gtgctgtcgg ccacaaaaat atgtaacgac ttcacgtcat ccagcatcac tttcgccact
     9181 gacccgctgc ccatttgctg taacacgcca ggcggaagct gcaatgcttt acgggctaac
     9241 ctgctgcgca gaatgttctc cagatgaaaa gccgcgtaat gagaggtgtt gaacgcctgt
     9301 aagcgcagaa caaaagttaa gaccacgcag ccgatcacgc cgcccagcgg ccaggccgga
     9361 atagcgtcag gcgtcgcgcg aatgtcacgc aggctccacg ccagaaaaag gagcgccccc
     9421 aggctggtta atgccgccag ccccgccagc gccatagcta aaataacctt cctgcgaacc
     9481 ggtgtaatca cctgccaaag aggaaatcgg gacagcgatt cagtattgga tgattgagat
     9541 gacatgggag taactgaatt tcctgatgaa tttttacgga cgccaaaaac gacaattcag
     9601 ggtggcgttc tgagaattaa tgtgaataat aaccattatc aataactcat ctaccccatt
     9661 cggttctatt tatacccgca ttggtctaag ccacagggag ataaccaggt catgacggag
     9721 tcaccgcaaa cgcaatctga aatctctatt caccagttgg tggtcgggaa acctgcaaac
     9781 gatggcaata ttcctgctca gtgtgaactg ctgcgctgtt cgttgcagga aggtatggat
     9841 attttgctct ggaggggcca ttttgcgcgg cctgagacgt tgcagctaca cgacgatctg
     9901 ggcaggatta acttctcgtg catcctggaa ggcacatcgc gctttgcgat tcagggactc
     9961 cggcgtcaca ccgactggga gcttgcgcgc aatcgccatt acattaccca tacgccagac
    10021 tgtcgcggca gcgcctccta ttgtggccga ttcgagagca ttaccctctc cttcagccct
    10081 gaaaccctcg cgctgtgggt gccggatatt agcgcggtca ttaagaataa gatcgactcc
    10141 cactgctgtt gccagcagca ccgttgtaat gctgaaaccc acttaacggc tcaggccttg
    10201 cgtcacgcgc tgatgcgcat gcacagcggc ttcagtcatg aacaaaagcc gtcgacgcta
    10261 tggttgcagg ggcaaagtct ggtcatgctc agtctggtgc tggatgaaca tcgcgaagac
    10321 gccagttgcc tctcctgcca cttcaccccg atggaacgcc agaaactgct gcgggcgaag
    10381 gatctgctgc tggctgatct gacgcaagcg ccgggcgtcg cggagctggc cagagagtcc
    10441 ggtctgagcg ttctcaaaat taagcgaggc ttccgcgttc tgttcaacaa cagcgtctat
    10501 ggcctgtttc aggctgaacg aatgcaggaa gccagaagac gcctggccaa cggcaatacc
    10561 tccgtcatga cggtggcggc cgatctgggc tatgccaacg ccagccattt ctctgccgcc
    10621 ttccagaaac agtttggcgt aactccttcg acctttaaac gcgggatgta accggggtcg
    10681 cgccccccta aactcctccc tgacagaggc ccggataccc catcagggcc tctgttcttt
    10741 ccgcctgtta ccaacccgaa acgggcataa ataaacccgt ttcgggtagc atccaattgt
    10801 taataattat tattctcata tgagtaatgc ttttcggtaa gacgtgccat caggaggaag
    10861 aatgatttct ggcgcaccat ctcaggattc gctgttaccg gacaaccgcc acgcggctga
    10921 ttaccaacaa ttacgcgagc ggctcataca ggaactgaat ttaacgccgc agcagttaca
    10981 tgaagagagc aacctgatcc aggccggcct ggattccata agattgatga gatggttaca
    11041 ctggtttcgt aaaaattgct accgccttac ccttcgcgag ctgtatgccg cccccacgct
    11101 ggcggcatgg aaccagttaa tgctcagccg gtcgccggag aacgcggaag aagaaacgcc
    11161 gcccgacgaa tcatcctggc cgaacatgac cgaaagtacc cccttcccat tgacgccagt
    11221 acagcacgcc tacctgacgg gccgcatgcc ggggcagacg cttggcggcg tgggttgcca
    11281 cctgtatcag gagtttgaag gccattgtct gacggcgtcg cagctggagc aggccatcac
    11341 gaccttgctg caacgccacc caatgctgca tatcgccttt cgccccgacg ggcagcaggt
    11401 ctggctaccg caaccttact ggaacggcgt caccgttcat gatttacgcc ataacgacgc
    11461 tgaaagccgc caggcctatc tggacgcact gcgccagcgc ctgagccacc gtcttttacg
    11521 cgtggaaatc ggcgaaacgt ttgattttca gctgacgctc ttgccggaca atcgccaccg
    11581 cctccatgtc aatattgacc tgctgattat ggatgcctcc agctttacgc ttttcttcga
    11641 tgagcttaac gccctgctgg ccggagaatc gctgccggct atcgacaccc gctatgattt
    11701 ccgctcgtat ttgctgcacc agcagaagat caatcaacca ctgagagacg acgcgcgcgc
    11761 ttactggctg gcgaaagcat cgacgcttcc ccccgcgccc gtcttgccgc tggcctgcga
    11821 acccgccacg ctacgtgaag tccgtaatac ccgacgccgc atgattgtcc cggcaacacg
    11881 ctggcacgcc tttagcaacc gggccggcga gtatggcgtg acgccgacaa tggcactggc
    11941 gacctgtttt tctgccgtgc tggctcgctg gggcggcctg acgcgtctgc tgcttaacat
    12001 caccttattc gaccgccagc cgctgcaccc ggcggttggc gcgatgcttg ccgacttcac
    12061 caatattctt ctgctggata ccgcctgcga tggcgatacc gtcagcaacc tggcgcgtaa
    12121 aaaccagctc acgtttacgg aggactggga gcatcgccac tggtccggcg tcgaattact
    12181 ccgtgaactc aaacgccagc agcgctaccc ccacggcgcc ccggtggtat ttaccagcaa
    12241 tctggggcgt tccctctaca gcagccgcgc agaatcgccg ttgggcgagc cggaatgggg
    12301 catctcgcaa acgccgcagg tctggataga tcatctggcg ttcgagcatc acggcgaggt
    12361 ctggctacaa tgggacagca acgacgcgct gttccctccg gcgttagtcg aaacattgtt
    12421 cgatacctac tgccagttga ttaaccaact ctgcgatgac gaaagcgcct ggcaaaagcc
    12481 gttcgcagat atgatgcccg ccagccagcg cgcgatacgc gaacgggtca acgccaccgg
    12541 cgcccccatt cccgaaggct tgctgcatga aggcattttc cgtatcgctc tgcaacagcc
    12601 gcaggcgctg gcggtaacgg acatgcgtta tcagtggaat tatcatgagc tgacagacta
    12661 tgcccgccgt tgcgcgggca ggttaatcga gtgcggggtt cagcccggcg ataatgtggc
    12721 tatcacgatg tcgaaaggtg caggacaact tgttgcggtt ctggccgtcc tgctggctgg
    12781 ggcggtttac gttccggttt cgctggatca gcctgccgca cggcgcgaga aaatctacgc
    12841 tgacgccagc gtccggctgg tgctcatttg tcagcacgac gccagcgccg ggtcagacga
    12901 tattcccgtc cttgcctggc agcaggccat tgaggcggag ccgatcgcca acccggtagt
    12961 acgcgccccc acgcaaccgg cctacattat ctacacctcc ggctctaccg gtacgccgaa
    13021 aggggtagtc atttctcacc ggggagcgct taacacctgt tgcgatatca atacccgcta
    13081 tcaggttggc ccgcatgaca gggtgctggc cctctccgcc ctacattttg atttatcggt
    13141 ttacgacatt tttggcgtac tgcgcgcggg cggcgcgctg gtgatggtga tggaaaatca
    13201 acggcgcgat cctcacgcat ggtgtgagct gatccagcgc catcaggtca cgctctggaa
    13261 cagcgtcccg gcgctgttcg atatgctgct gacctggtgt gaaggtttcg ccgacgccac
    13321 gccggaaaac ctgcgcgcag tgatgctttc cggcgactgg atcgggcttg acctccccgc
    13381 ccgttatcgg gccttccggc cacaaggaca atttatcgcg atgggcggtg ccaccgaggc
    13441 gtctatctgg tctaacgcct gcgaaattca cgacgtcccc gcccactggc gctccatccc
    13501 ttacggtttt ccgctaacca accaacgcta ccgggtggtg gatgaacagg gccgggactg
    13561 ccctgactgg gtgccgggtg aattatggat tggcggcatt ggggtcgcgg aaggctattt
    13621 caacgatccc ctgcgtagcg agcagcaatt tttgacgctc ccggacgagc gctggtatcg
    13681 caccggcgat ctcggctgct actggccaga tggcacaatc gagttcctcg gtcgtcgcga
    13741 caagcaggtc aaagtcggag gatatcgcat cgagctgggc gaaatcgaaa gcgcgctcag
    13801 ccagctggcg ggggtgaaac aagcaaccgt tctggcgatc ggcgaaaaag aaaaaacgct
    13861 ggcggcatac gttgttcctc agggcgaggc tttttgcgtt accgatcatc ggaacccggc
    13921 actgccgcag gcgtggcaca cgcttgcggg aacgttgccc tgttgcgcca tctcgccaga
    13981 gatctccgca gaacaggtag ccgatttcct tcagcatcgc ctgctaaaac tgaagccggg
    14041 tcacaccgct ggcgccgatc ctctccccct gatgaactca ctcgctatcc agccgcgctg
    14101 gcaggccgtg gtggaacgct ggttagcatt tctggtgaca caacggcgac tgaagcccgc
    14161 tgctgaaggt tatcaggtct gcgctggtga agaacgcgag gatgagcacc cgcacttcag
    14221 cggacatgat ttaacgttat cgcaaattct tcgcggtgcc cgtaacgaac tgtcgttact
    14281 gaacgacgcg cagtggtcgc cggaaagcct ggcctttaac catccggcca gcgccccgta
    14341 tattcaggaa ctggcgacaa tttgccaaca gcttgcacag cgcttacagc gcccggtgcg
    14401 cctgcttgag gtgggaaccc gcactggccg cgccgcagaa tcgctgttag cacagctcaa
    14461 cgccggacag attgagtatg tcgggcttga gcagagccag gagatgctgc tgagcgcccg
    14521 gcagaggctc gccccctggc ctggcgcccg tctgtccctc tggaatgcag acacgctggc
    14581 ggcgcacgct cactcggcgg acattatctg gcttaataac gccctgcatc gtctgctgcc
    14641 ggaagatccc gggctccttg cgacattaca acagcttgcc gttcccggcg cgctgctcta
    14701 cgtgatggag tttcgccagt taacgccgtc cgccctactc agcacgctcc tgttaaccaa
    14761 tgggcagccg gaggccttgc tgcataacag cgccgactgg gcggcattat ttagcgcggc
    14821 cggcttcaac tgtcagcatg gcgatgaggt cgcggggtta caacgcttcc tcgtacaatg
    14881 tcctgacagg caggtgcgcc gcgatccccg tcaacttcag gccgccctcg ccgggcgtct
    14941 gccggggtgg atggtgccgc aacggatcgt cttcctcgac gccttaccgc tgacggctaa
    15001 cgggaaaatt gactaccagg cgctgaagcg tcgtcatacc cctgaagcgg aaaacccggc
    15061 cgaagcggat ttaccccagg gcgacattga aaaacaggtt gccgccctct ggcagcaact
    15121 cttatcaact ggcaatgtca ccagagaaac cgacttcttc cagcaaggcg gcgatagcct
    15181 gctggcgacc cgtctgaccg ggcaacttca tcaggcaggt tatgaagcgc aattaagcga
    15241 cctgtttaat catccccggc tggcggattt tgccgccacg ctgcggaaaa ccgacgtccc
    15301 ggtcgaacaa ccattcgtcc actcccctga agatcgctac cagccctttg cgcttaccga
    15361 cgtgcagcag gcttacctag tggggcgtca gccgggcttt gccctgggcg gcgtcggctc
    15421 acatttcttt gttgaatttg aaattgccga tctggatctc acccggttgg agacggtctg
    15481 gaaccgatta atcgcccgcc acgatatgct acgcgccgtc gtgcgtgatg gacagcaaca
    15541 ggtgctcgaa cagacgcctc gctgggtaat acccgcacac atcctccata cgcctgaaga
    15601 ggcgttgcag gtgcgcgaaa aactggccca tcaggtactc aaccccgaag tctggccggt
    15661 attcgatctc caggtcggtt acgttgacgg gatgcccgcc cgcctgtggc tgtgtctgga
    15721 taacctgtta cttgacgggc tgagcatgca gattctgttg gcggagctgg agcacggcta
    15781 ccgctacccg caacagctgc ttccgccgct gcccgtcacc ttcagggatt atctgcaaca
    15841 accctcgcta cagtcgccca atccagattc tctggcatgg tggcaggcgc agcttgatga
    15901 tattcctccg gcgcctgcgt tgccgctgcg ctgcttgcct caggaggttg aaacaccgcg
    15961 cttcgcccgc ctgaacggcg cactggacag cacgcgctgg catcggctga aaaaacgggc
    16021 ggctgacgcc catctcaccc cgtcggccgt actgttgtcg gtgtggtcaa cggttctctc
    16081 tgcatggagt gcacagcctg agttcacgct taaccttacg cttttcgaca ggcgaccgct
    16141 gcacccgcaa atcaaccaga ttctgggcga tttcacctcg ctgatgctgc tgagctggca
    16201 tcccggcgaa agctggctgc acagcgcgca gtcactacag cagcggctga gccagaacct
    16261 caaccaccgc gatgtgtcag ccatccgcgt gatgcgtcaa ctggcgcaac ggcaaaacgt
    16321 gccagccgtt ccgatgcccg tcgtctttac cagcgcgctg ggctttgagc aggataactt
    16381 cctcgcccgg cgtaatctgc tcaaaccggt ctggggcatc tcccagacgc cgcaggtctg
    16441 gctcgatcac cagatttatg aatccgaagg cgaactgcgc tttaactggg attttgtcgc
    16501 cgcgctgttt cctgccgggc aggtggagcg ccagtttgaa cagtattgcg cattgctaaa
    16561 ccgaatggcc gaggatgaaa gcggctggca actgccgctc gccgcgctgg tgcctcccgt
    16621 taaacacgca gggcaatgcg cagagcgctc accgcgcgta tgccctgagc actctcagcc
    16681 acacattgcg gcggacgaga gcaccgtcag cctgatttgc gacgccttcc gcgaggtggt
    16741 tggcgagtct gtcacgcccg cagaaaactt ctttgaggcg ggcgcaacgt cgctgaatct
    16801 ggtgcaactg cacgttttgt tacaacgtca cgaattttcc accctgacgt tgcttgacct
    16861 cttcacccac ccttctcctg ctgccctggc cgattatctg gccggcgtcg ccacggtgga
    16921 gaaaacaaaa cgacctcgcc ctgttcgccg tcgtcagcgg cggatatagc gcgaagcaaa
    16981 ctgattttcc ccggaacgcc atcgcgaacg catggcgttc cattgacttt gtgaatctta
    17041 ggaaacggga ccgattatgg ataacttgcg cttctcttct gcgccgacag cagattccat
    17101 tgatgcatcg atcgctcaac actacccgga ctgcgaacct gtcgcggtta tcggctacgc
    17161 ctgccatttt cctgaatcgc cggatggcga aacgttctgg caaaatctgc tggaaggtcg
    17221 tgaatgcagc cgacgcttta cgcgcgaaga gcttctggcc gtcggtctgg atgccgccat
    17281 cattgacgat cctcattatg tcaatatcgg tacggtgtta gacaacgccg actgcttcga
    17341 cgccaccctg tttggctatt cgcgacagga agcggagtcg atggacccgc agcagcgcct
    17401 gtttttgcag gcggtctggc atgcgctgga acatgccggt tatgcccccg gcgccgtccc
    17461 ccataagacc ggcgttttcg cctcttcccg gatgagtacc taccccggtc gcgaagcatt
    17521 gaacgtgaca gaagtcgcgc aggtaaaagg tctgcaatct ctgatgggca atgataaaga
    17581 ctatattgcc acccgcgccg cgtacaaact caacctgcac ggcccggcgt tatcggtaca
    17641 gaccgcctgc tccagctcgc tggttgccgt gcatctggcc tgtgaaagcc tgcgcgcagg
    17701 cgaatccgat atggcggttg ccggcggcgt ggcgctctct ttcccccagc aggcaggcta
    17761 ccgctaccag cccggaatga ttttctctcc tgatggtcac tgtcgtccct ttgacgcctc
    17821 ggctgagggc acctgggccg gtaacggtct cggctgcgtg gtgctgcgtc gcctgagaga
    17881 cgcgctgctg tcaggcgatc cgattatctc ggtgatcctc tccagcgcgg tcaacaacga
    17941 cggcaacaga aaggtcggct ataccgcccc ttccgtcgca gggcaacaag cagtcatcga
    18001 agaggcgtta atgctggcgg ccatcgacga caggcaggta ggttacattg aaacccacgg
    18061 caccggcaca ccgctgggcg acgcgattga aattgaagcg ttacgcaacg tctatgcgcc
    18121 tcgcccgcag gatcagcgct gtgcgctcgg ttccgtgaaa agtaacatgg gccatctgga
    18181 taccgcggcg ggcattgccg gactgctgaa aaccgttctg gcagtcagtc gcgggcaaat
    18241 tcctccctta ctgaattttc acacccccaa cccggcgctg aaacttgaag agagcccctt
    18301 taccataccg gtgtcggcac aggcatggca ggacgaaatg cgctatgcgg gcgtctcctc
    18361 ctttggtatt ggcggcacca actgccatat gatcgtcgcc tcgctgcccg acgcgctcaa
    18421 cgcgcgcctc cccaatacgg atagcggcag aaaaagtacc gcgctgctgc tcagcgccgc
    18481 cagcgacagc gcgttgcggc ggctggcgac ggattatgcc ggggcgctga gagagaatgc
    18541 ggatgccagc tctctggcct tcacagccct gcacgcgcgc cgtctcgatc tccccttccg
    18601 cctggcggcg ccattaaacc gtgaaaccgc cgaggcgctc agcgcctggg ccggtgagaa
    18661 atcgggggcg ctggtttaca gcggccacgg cgccagcggc aagcaggtgt ggctgtttac
    18721 cggccagggc tcgcactggc gcactatggg tcaaacgatg taccagcact caacggcgtt
    18781 tgccgacacg ctggatcgct gtttttccgc ctgtagcgaa atgctcacgc cgtcactgcg
    18841 cgaagcgatg tttaaccccg attcggcgca gctggacaat atggcctggg cgcagccggc
    18901 gattgtcgcg tttgaaatcg cgatggcggc gcactggcgt gctgaaggac tgaagccaga
    18961 cttcgccatt gggcattccg tcggtgaatt tgccgctgcc gttgtctgcg gacactatac
    19021 gattgaacag gtcatgccac tggtttgtcg gcgcggcgcg ctaatgcagc agtgcgcaag
    19081 cggcgcgatg gtggcggtat ttgcagacga agacacgctg atgccgctgg ctcgccagtt
    19141 tgagctggat ctcgccgcca acaacggtac gcaacatacg gtattttccg ggccggaagc
    19201 ccgtctcgcg gtattttgcg ccacgctctc gcagcatgac attaactatc gtcgcctgag
    19261 cgtaaccggt gcggcgcact ccgctttact ggagccgata ctcgatcggt tccaggacgc
    19321 ctgcgcggga ctgcacgcgg agccggggca aataccgatt atttccacgc tcaccgccga
    19381 cgtcattgat gagtcaacgc tcaaccaggc ggattactgg cgccgacaca tgcgccagcc
    19441 ggtgcgtttt atccagagta ttcaggtggc gcatcagctc ggcgcccgcg tttttctgga
    19501 gatggggccc gatgcccagt tggttgcttg cgggcagcgc gaataccgcg ataacgcata
    19561 ctggatagcc agcgcccggc gtaacaaaga ggcgagcgat gtcctcaatc aggccctgct
    19621 ccagctttac gctgccggcg tcgccctacc gtgggccgac ctgctggcgg gcgatggaca
    19681 acgtatcgct gcgccatgtt atccgtttga tactgaacgt tactggaaag agcgcgtctc
    19741 cccggcctgc gagcctgccg acgcagcgct gtctgccggg ctggaggtgg cgagtcgcgc
    19801 cgcgacagcg ctcgatctcc ctcgcctgga tgcgcttaaa cagtgcgcca cgcgactgca
    19861 cgccatctac gtcgatcaac tggtacaacg ctgtaccggc gatgccattg agaacggcgt
    19921 ggacgccatg accatcatgc gccgtggacg tctgctgccc cgctaccagc agctactcca
    19981 gcgcctgctg aataactgcg tggtcgacgg cgattaccgc tgcaccgacg ggcgatacgt
    20041 ccgcgcccgc cccattgaac atcaacagcg ggaatcactg ctgacggaac ttgccggtta
    20101 ttgtgaaggt tttcaggcta ttcccgacac catcgcccgt gccggcgatc ggttatatga
    20161 aatgatgagc ggcgcggaag aaccggtggc gattatcttc ccgcaaagcg cctccgacgg
    20221 cgtggaagtg ctgtatcagg aattcagctt tggccgctat ttcaaccaaa tcgccgccgg
    20281 ggtattacgc ggcattgtcc agacgcgtca gccccgccag ccgttgcgta ttcttgaagt
    20341 tggcggcgga accggcggca ccaccgcgtg gctgctgccg gaactcaacg gcgttccggc
    20401 actggagtac catttcaccg atatctcggc gctgttcacc cgtcgcgccc agcagaaatt
    20461 cgccgactat gattttgtga agtatagcga gctggatctc gaaaaagagg cgcagtctca
    20521 gggtttccag gcacagtctt acgatcttat cgtggcagcg aacgtgattc acgccacccg
    20581 ccatattggc cgcacgctcg ataatctgcg ccccctgctc aagccgggcg ggcgcctgct
    20641 gatgcgcgaa atcacccagc caatgcgtct gtttgacttc gttttcggcc cgctggttct
    20701 tccgctacag gatctcgacg cccgcgaagg tgagttattc ctcaccaccg ctcagtggca
    20761 acaacagtgc cgccacgccg gattcagcaa agtggcgtgg ctaccgcagg atggcagccc
    20821 gaccgccggg atgagcgaac atatcattct cgccacgctg cccggtcagg cggttagcgc
    20881 cgtaacattc accgcgccat cagaacccgt gttggggcag gcgctgacgg ataacggtga
    20941 ttatctcgcc gactggtctg attgcgcagg tcagcccgaa cggtttaacg cccgctggca
    21001 ggaggcatgg cgtctgcttt cacagcgtca tggcgacgct ctgcctgtgg aaccgccccc
    21061 cgtcgccgcc ccggagtggc tggggaaggt tcgcttaagc tggcaaaacg aagccttttc
    21121 ccgcggtcag atgcgcgttg aagcccgtca tcctgctggc gagtggctgc cgctatcgcc
    21181 cgccgcgcct cttcctgcgc cgcaaacgca ttatcaatgg cgctggacgc ccctcaacgt
    21241 cgccagcatt gaccatccgc ttacctttag cttcagcgcc ggtacgcttg cgcgcagcga
    21301 cgagctggcg caatacggca tcattcacga tccgcacgcc tcttcacgac tgatgattgt
    21361 tgaggagagc gaggatacgc tggccttagc ggagaaagtg atagcagcgc tcaccgccag
    21421 cgcagccgga ttgattgtgg ttactcgccg cgcgtggcga gtcgaggaaa atgaagcact
    21481 ctctgcatcc catcacgcgc tatgggcctt gcttcgcgtc gcggccaacg aacagccgga
    21541 acggttgctt gccgccatcg atctcgccga aaacaccccg tgggaaacgc tgcatcaagg
    21601 gttgagcgca gtctcactat cacagcgctg gctcgccgca cggggtgaca ccctttggct
    21661 cccttcactg acgcccaata cgggatgcgc cgctgaatta ccggcaaacg tgtttaccgg
    21721 cgatagccgc tggcatctgg tgaccggagc gtttggcgga ttaggccgcc ttgccgtgaa
    21781 ctggctcaga gaaaaagggg cgcgacgcat cgccctgctg gcaccgcgcg tggatgagtc
    21841 atggctacgc gacgtggagg gcgggcagac gcgcgtctgc cgttgtgatg tgggcgatgc
    21901 cgggcaactg gccacggttc ttgacgatct ggcggccaac ggcggcattg ccggagcgat
    21961 tcatgccgct ggcgtattgg ctgacgcgcc cttgcaggag cttgatgacc accagctggc
    22021 tgccgttttc gcggtaaaag cgcaggcggc aagccaactg ttgcaaaccc tgcgcaacca
    22081 cgacggacgc tatcttattc tctactcttc cgctgccgcc accctcggcg cgccgggtca
    22141 gagcgcccat gcgctggcct gcggctacct ggacgggctg gcccagcagt tttccaccct
    22201 tgatgcgccg aaaacgctct ctgtcgcctg gggcgcatgg ggagaaagcg gtcgggcggc
    22261 cacgccggaa atgctggcga cgctcgccag ccgaggtatg ggcgcgttaa gcgatgccga
    22321 aggctgctgg cacctggaac aggcggtgat gcgcggcgcc ccgtggcgac tggcgatgcg
    22381 cgtttttacc gacaaaatgc ccccgttaca acaggctctg tttaacatca gcgccacaga
    22441 aaaagccgca acgccggtca ttcctcctgc tgatgacaac gcctttaacg gcagcctgag
    22501 cgatgaaaca gcggtgatgg catggctgaa aaagcggatt gcggttcagc taaggctgag
    22561 cgatccggcg tcactgcatc caaaccagga tctgttgcaa ctcggcatgg actcgctgct
    22621 cttccttgaa ctcagtagcg atattcagca ctacctgggt gtacgcatca atgcggaacg
    22681 ggcgtggcag gatctgtctc ctcatggact cacgcagctt atctgttcta agccagaggc
    22741 gacgcctgcc gcttcgcagc cggaagtatt gcggcacgac gccgacgagc gttatgcgcc
    22801 cttccctttg acgcccattc agcacgccta ctggctgggg cgaacccacc tcattggcta
    22861 tggcggcgtc gcctgtcacg tcctgtttga gtgggataaa cgccacgatg agttcgatct
    22921 cgccatactg gagaaagcat ggaaccagct catcgcacgc cacgatatgt tgcgtatggt
    22981 ggttgatgcc gacgggcagc agcgaatcct ggcgacaacg ccggagtatc acatcccgcg
    23041 tgacgatctg cgcgcgcttt ccccggaaga acagcgcatc gcactggaaa aacggcggca
    23101 tgaactgagc tatcgcgttt tgcctgccga ccagtggcct ctttttgagc tggtggtcag
    23161 cgaaatcgac gattgccatt accgtctgca tatgaacctc gaccttttgc agtttgatgt
    23221 gcagagtttt aaagtcatga tggacgacct ggcgcaggtc tggcgcggtg aaacgctggc
    23281 gccgctcgct attaccttcc gtgattatgt gatggctgaa caggcgcgcc gacagacatc
    23341 ggcatggcac gatgcctggg attactggca ggaaaaactg ccgcaactgc ccttagcgcc
    23401 agagctgccg gtggttgaga cgcccccgga aacgccacac ttcaccacct tcaaatcgac
    23461 gatcggcaag acagaatggc aggccgtgaa acagcgctgg cagcagcaag gcgtcacacc
    23521 gtctgccgcg ctgctcacgc tgtttgccgc cacccttgag cgctggagcc gtaccacaac
    23581 atttacgctg aacctgacgt tcttcaatcg ccagccgatc catccgcaaa tcaaccagtt
    23641 gattggtgat tttacctccg tcacgctggt tgattttaac ttctcagcgc cggtgacgtt
    23701 gcaagagcag atgcaacaga cccaacagcg cctctggcaa aacatggcgc acagtgaaat
    23761 gaacggtgtt gaggtgatcc gtgagctggg ccgcctgcgc ggatcacaac gtcaaccgct
    23821 gatgccggta gtgtttacca gtatgctggg gatgacgctg gaaggcatga ctatcgatca
    23881 ggcgatgagc catctgttcg gcgaaccctg ctatgtattc acgcaaacgc cgcaggtctg
    23941 gctggatcat caggtcatgg agagcgacgg cgagttgatg tttagctggt actgcatgga
    24001 caacgtgctg gaacccggcg ctgccgaggc gatgtttaat gactattgcg ccatcctgca
    24061 agccgtcatc gccgcccctg aaagcctgaa gactctcgcc agcggcatcg ccaggcacat
    24121 tccccgccga cgctggccgc tgaacgcgca ggcggactac gacctgcggg atattgagca
    24181 ggcgacgctc gaataccccg gcatccggca ggccagagcg gaaataaccg aacagggcgc
    24241 gttgacgctg gatatcgtga tggccgacga tccttcgcca tcagcggcga tgcctgatga
    24301 gcacgaactt acccaactgg cgctgccgtt gcctgagcag gcgcagcttg atgagctgga
    24361 ggcgacctgg cgctggctgg aggcgcgtgc gctacagggg atcgcggcta cgctaaatcg
    24421 tcacggcctg tttaccacgc cggagatcgc ccatcgcttt agcgcagtag tacaggcgct
    24481 gtccgcgcaa gcgtctcacc agcgtctgct gcgccagtgg ctacagtgtc tgacggaaag
    24541 agagtggtta atccgcgaag gtgaaagctg gcgctgccgc attccgctca gcgagattcc
    24601 tgagcctcag gaagcgtgcc cgcaaagcca atggagccag gcgctggcgc agtatctgga
    24661 aacctgcatc gcccggcacg acgccctctt ctccgggcag tgttctccgc tggaattgct
    24721 gttcaacgag cagcatcgcg ttaccgacgc gctgtatcgc gacaaccccg ccagcgcctg
    24781 tctgaatcgc tataccgcgc agattgccgc cttgtgcagc gcagaacgga ttctggaggt
    24841 tggcgccgga accgcagcca ctaccgcgcc ggtgctgaag gccacgcgga acacgcggca
    24901 gtcgtaccac ttcacggacg tctccgcgca gttcctcaat gacgccagag cccgtttcca
    24961 tgatgaatcg caggtgtctt atgccttgtt cgacatcaac cagccgctgg atttcaccgc
    25021 ccacccggag gcgggttacg acctgatcgt tgccgtcaat gtgctccacg acgccagcca
    25081 tgtcgtccag acgttgcgca gattaaaact gttgctgaaa gccggcggac gtttgctgat
    25141 cgttgaagcg acggagcgaa acagcgtatt ccagctggcg agcgtgggct ttattgaggg
    25201 attaagcgga taccgcgatt tccgccgccg ggatgagaaa ccgatgctca cccgctccgc
    25261 atggcaggag gttctcgttc aggccgggtt tgcaaacgag ctggcgtggc ccgcgcagga
    25321 atcgtcgccg ctgcgccagc atctgctggt agcgcgttcg cctggcgtaa atcgcccgga
    25381 taaaaaagcc gtgagccgct atttacagca gcgctttggc accggtctgc ccattttaca
    25441 gatccggcaa agagaagcgt tatttacgcc gctgcatgcc ccgtctgatg cgccgactga
    25501 gccagccaaa cccacgccag ttgccggggg gaatccggca ctggaaaaac aggtggctga
    25561 actctggcaa tcgctgctgt ctcgccccgt ggcaaggcat cacgactttt tcgaactggg
    25621 cggcgacagc ctgatggcga caaggatggt cgcgcagcta aaccggagag ggattgccag
    25681 ggctaacctt caggatctgt tcagccattc gacgctgagc gacttctgcg cccatctaca
    25741 ggcggctacg tcaggagagg acaacccgat acccctttgc cagggcgacg gtgaggaaac
    25801 cctgtttgtc ttccacgctt cagacggcga tatcagcgcc tggctgccgc tcgctagcgc
    25861 gttgaacagg cgcgttttcg gcctgcaagc aaaatcgccg cagcgctttg ccacgctcga
    25921 ccagatgatc gatgagtatg tcgggtgcat ccgtcgtcag cagcctcacg gcccttatgt
    25981 gctggcgggt tggtcgtatg gcgcgtttct tgcggcgggc gccgcacaac gcctgtacgc
    26041 caaaggcgag caggttcgga tggtgttaat cgatcccgtg tgccgacagg atttctgttg
    26101 cgaaaaccgg gcggccctgc tgcgcctgtt agccgaagga caaacgcctc tggcactgcc
    26161 cgaacatttc gaccagcaga cgcccgacag ccagcttgcc gactttatca gcctcgctaa
    26221 aacggccggt atggtgtcgc aaaacctgac gctgcaagcg gcagaaacgt ggctcgacaa
    26281 catcgcgcat ctgctgcgtt tactgactga gcatacgccg ggcgaaagcg ttccggtccc
    26341 ctgtctcatg gtgtatgccg ccgggagacc cgcgcactgg acgccagcag aaaccgagtg
    26401 gcagggctgg ataaacaacg ccgacgacgc tgtgattgaa gccagccact ggcaaatcat
    26461 gatggaagcc ccccacgttc aggcttgtgc gcaacacatt acgcgctggc tttgcgcaac
    26521 ctcaacgcaa ccggagaaca cgttatgatg ccgtccgcct ccccaaaaca acgcgtactg
    26581 attgtgggcg ccaaatttgg cgaaatgtac ctgaatgcct ttatgcagcc cccggagggg
    26641 ctggaactgg tcggcctgct ggcgcaggga agcgcccgtt caagagagct ggctcatgcg
    26701 tttggcattc cgctgtatac ctcgccggaa cagataacca ggatgccgga tatcgcctgt
    26761 attgtcgtgc gttcgaccgt cgccggagga accggtacgc agcttgccag acactttctg
    26821 acgcgcggcg tacacgtcat tcaggagcat cccctccacc cggatgacat cagttcctta
    26881 caaacgctgg cgcaggaaca gggctgctgc tattgggtaa acacgtttta tccgcatacg
    26941 cgcgcgggac gcacatggct tcgcgatgcg cagcagctgc gtcgctgtct ggctaagacg
    27001 ccgcaggtgg tccacgccac caccagccga cagttgctct actccactct ggatttgttg
    27061 ttgctggctc tgggcgttga tgccgccgcc gtggagtgcg acgtggttgg cagctttagc
    27121 gattttcact gtctgagact cttctggccg gagggagaag cctgcctgct gcttcagcgc
    27181 tatctcgatc ctgacgatcc agatatgcat agccttatca tgcaccgcct gctgctgggc
    27241 tggccggaag gtcatctttc gctggaggcg agctacggcc ccgtcatctg gtcatccagc
    27301 ctgtttgtcg cagaccatca ggagaacgcc cacagcctct accgcagacc ggagatcctg
    27361 cgcgatctgc cgggtctgac gcgcagcgcg gcgcccctga gctggcgcga ctgctgcgaa
    27421 accgtcgggc cggagggcgt cagctggttg ctacaccagt tgcgcagcca tctggcgggc
    27481 gaacatccac cagcggcctg ccaaagcgta catcagatcg cgttatcacg tttatggcag
    27541 cagattttac gtaagaccgg caatgcggag atcagacgcc tgacgccgcc gcaccacgac
    27601 cggttagccg gtttctacaa cgatgatgat aaggaggcgc tgtgacgcaa tctgcaatgt
    27661 gcatcccgct gtggcccgcc cggaacggca atactgcgca tctggtcatg tgccctttcg
    27721 ctggcggcag cagtagcgcg tttcgccact ggcaagctga gcaactggct gattgcgcgc
    27781 tttctctggt gacctggccg gggcgcgatc gccttcgcca tctggaaccg ctcagaaaca
    27841 ttacacaact ggcggcactg ctggcgaacg agctggaagc atccgtatcg cctgacacgc
    27901 cgcttttact cgccgggcac agcatggggg cgcaggtggc gtttgaaacc tgccgacttc
    27961 tggagcaacg ggggcttgcg ccacaaggac tgattatttc cgggtgccat gccccgcatc
    28021 tgcattctga acgccagctc agccatcgcg atgatgccga ctttatcgct gagctgatag
    28081 acattggcgg atgttctcct gaactgcggg aaaaccagga attaatgtcg ctgtttcttc
    28141 ctcttctgcg cgctgatttt tacgccaccg agagctatca ctacgactcg cccgacgtct
    28201 gtccgccgct gcgcacgcct gcgctgttat tgtgcggcag ccacgatcgc gaagcctcct
    28261 ggcagcaggt cgatgcctgg cgtcagtggc tgagccacgt tacaggcccg gtggtgattg
    28321 acggcgatca tttctatccc attcaacaag cccggtcctt ttttacgcag attgtccgcc
    28381 attttcccca cgcattttct gcaatgaccg cgtggcaaaa acagcccagt acttcagaaa
    28441 ggtgacgcat gaattcttcc tttgaatctc tgattgaaca gtatccctta cccattgccg
    28501 aacagttgcg ccactgggcg gcccgttatg cctcgcgaat tgccgtcgtt gatgcaaagg
    28561 ggtcgttaac ctacagcgcg cttgatgcac aagttgacga acttgccgca ggtctgtcat
    28621 cactgggttt gcgttcgggg gagcatgtaa ttgtgcagct tcccaacgac aacgcgtttg
    28681 ttaccctgct gttcgccttg ttaagactgg gcgttatccc cgtgctggcg atgccctcgc
    28741 aacgggctct ggatatcgac gcgctgattg agctggcgca acccgtcgct tacgttattc
    28801 acggggaaaa cctcgcagag ctggcccgac agatggcgca caaacacgcc tgcttgcgtc
    28861 atgttctggt cgctggagag accgtgagcg acgattttac gccgctcttc tcccttcacg
    28921 gtgagcgaca ggcatggccg cagcctgatg tttccgccac cgcgttgttg ttgctctcag
    28981 gcggcacaac cggcacgccc aaactcatcc cgcgccgaca tgccgactat agctataact
    29041 tcagcgcttc tgctgaactg tgcggcatca gccaacagag cgtgtatctc gccgtcctcc
    29101 cggtggcgca taactttccg ctggcctgcc ccggtattct gggaacgctt gcctgcggcg
    29161 gaaaagtggt gctgaccgac agcgccagct gtgatgaggt gatgccttta atcgcgcagg
    29221 aaagagtgac tcacgtcgcc ctggttccgg cgctggcgca attatgggtg caggccaggg
    29281 agtgggaaga cagcgacctt tcgtcgctgc gcgtcattca ggcaggcggc gcccggctcg
    29341 acccgacgct tgctgagcag gttatcgcca cctttgactg taccctgcaa caggttttcg
    29401 gtatggcgga aggcctgctc tgttttaccc ggctggacga tccgcatgcc accattctcc
    29461 acagccaggg gcgcccgttg tcccctctgg atgaaatccg catcgttgat caagacgaga
    29521 acgacgtcgc gccgggcgaa accgggcaat tgttaacgcg cggcccttat accatttcgg
    29581 gctattaccg cgcccctgcc cacaacgcgc aggcctttac cgcgcaaggg ttttaccgca
    29641 caggcgacaa tgtcaggctg gatgaggtgg ggaacctgca cgttgaggga cgcataaaag
    29701 agcagatcaa ccgcgccgga gaaaaaatag ccgcggctga agtggaatcg gcactgctgc
    29761 gtttagcgga agtgcaagat tgcgcggtgg tcgccgcgcc ggacacgctg cttggcgagc
    29821 ggatttgcgc gtttatcatc gcgcagcagg tgccaactga ctatcagcag ttgcgtcaac
    29881 aactgacccg tatggggctc agcgcgtgga aaattcctga ccaaatcgag tttctggacc
    29941 actggccgct caccgccgtc ggcaagatag acaaaaaacg cctgacggct ctcgccgtcg
    30001 accgttatcg ccattctgcc caataagcgc aaaccgaccc gaaacaggtt gaaataaacc
    30061 cgtttcgggt agcaccacta ttagaaatag ttatcatttt caattcacca ttgtcggtat
    30121 ttttggcgtt tcgccgtctt acagggactc acaacaatga aaatgacacg gctttatcct
    30181 ctggccttgg ggggattatt gctccccgcc attgctaatg cccagacttc acagcaagac
    30241 gaaagcacgc tggtggttac cgccagtaaa caatcttccc gctcggcatc agccaacaac
    30301 gtctcatcta ctgttgtcag cgcgccggaa ttaagcgacg ccggcgtcac cgccagcgac
    30361 aaactcccca gagtcttgcc cgggctcaat attgaaaata gcggcaacat gcttttttcg
    30421 acgatctcgc tacgcggcgt ctcttcagcg caggacttct ataaccccgc cgtcaccctg
    30481 tatgtcgatg gcgtccctca gctttccacc aacaccatcc aggcgcttac cgatgtgcaa
    30541 agcgtggagt tgctgcgagg cccacaggga acgttatatg gcaaaagcgc tcagggcggg
    30601 atcatcaaca tcgtcaccca gcagccggac agcacgccgc gcggctatat tgaaggcggc
    30661 gtcagtagcc gcgacagtta tcgaagtaag ttcaacctga gcggccccat tcaggatggc
    30721 ctgctgtacg gcagcgtcac cctgttacgc caagttgatg acggcgacat gattaacccc
    30781 gcgacgggaa gcgatgactt aggcggcacc cgcgccagca tagggaatgt gaaactgcgt
    30841 ctggcgccgg acgatcagcc ctgggaaatg ggctttgccg cctcacgcga atgtacccgc
    30901 gccacccagg acgcctatgt gggatggaat gatattaagg gccgtaagct gtcgatcagc
    30961 gatggttcac cagacccgta catgcggcgc tgcactgaca gccagaccct gagtgggaaa
    31021 tacaccaccg atgactgggt tttcaacctg atcagcgcct ggcagcagca gcattattcg
    31081 cgcaccttcc cttccggttc gttaatcgtc aatatgcctc agcgctggaa tcaggatgtg
    31141 caggagctgc gcgctgcaac cctgggcgat gcgcgtaccg ttgatatggt gtttgggctg
    31201 taccggcaga acacccgcga gaagttaaat tcagcctacg acatgccgac aatgccttat
    31261 ttaagcagta ccggctatac caccgctgaa acgctggccg catacagtga cctgacctgg
    31321 catttaaccg atcgttttga tatcggcggc ggcgtgcgct tctcgcatga taaatccagt
    31381 acacaatatc acggcagcat gctcggcaac ccgtttggcg accagggtaa gagcaatgac
    31441 gatcaggtgc tcgggcagct atccgcaggc tatatgctga ccgatgactg gagagtgtat
    31501 acccgtgtag cccagggata taaaccttcc gggtacaaca tcgtgcctac tgcgggtctt
    31561 gatgccaaac cgttcgtcgc cgagaaatcc atcaactatg aacttggcac ccgctacgaa
    31621 accgctgacg tcacgctgca agccgcgacg ttttataccc acaccaaaga catgcagctt
    31681 tactctggcc cggtcgggat gcagacatta agcaatgcgg gtaaagccga cgccaccggc
    31741 gttgagcttg aagcgaagtg gcggtttgcg ccaggctggt catgggatat caatggcaac
    31801 gtgatccgtt ccgaattcac caatgacagt gagttgtatc acggtaaccg ggtgccgttc
    31861 gtaccacgtt atggcgcggg aagcagcgtg aacggcgtga ttgatacgcg ctatggcgca
    31921 ctgatgcccc gactggcggt taatctggtc gggccgcatt atttcgatgg cgacaaccag
    31981 ttgcggcaag gcacctatgc caccctggac agcagcctgg gctggcaggc gactgaacgg
    32041 atgaacattt ccgtctatgt cgataacctg ttcgaccgtc gttaccgtac ctatggctac
    32101 atgaacggca gcagcgccgt cgcgcaggtc aatatgggtc gcaccgtcgg tatcaatacg
    32161 cgaattgatt tcttctgatt attgtaaaag ggataccgaa aaggtatccc ttttacacca
    32221 ctagttaaaa ccagtaactc agcagagtcg caaaaatatt aatccatagt gattatttaa
    32281 acaatgaaat tgcgattagg ataaatagat ttaac
//
LOCUS       DACADF010000043        31584 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.043, whole genome shotgun sequence.
ACCESSION   DACADF010000043 DACADF010000000
VERSION     DACADF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.043"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..475
                     /gene="agaB"
                     /locus_tag="ISB30_001879"
     CDS             <1..475
                     /gene="agaB"
                     /locus_tag="ISB30_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAP1989515.1"
                     /translation="SSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDDI
                     QQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDLK
                     DVNVGNMHFSEGKKQISSKVYVDDQDLTDLRYIKQRGVNVFIQDVPGDQKEQIPD"
     gene            514..1317
                     /locus_tag="ISB30_001880"
     CDS             514..1317
                     /locus_tag="ISB30_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylgalactosamine permease IIC component 1"
                     /protein_id="HAP1989516.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1307..2098
                     /gene="agaD"
                     /locus_tag="ISB30_001881"
     CDS             1307..2098
                     /gene="agaD"
                     /locus_tag="ISB30_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HAP1989517.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKNDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2099..2866
                     /locus_tag="ISB30_001882"
     CDS             2099..2866
                     /locus_tag="ISB30_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HAP1989518.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFHSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTADRPVTRGITLGLKDILNAREVLLLITGEGK
                     QDATERFLTAKVSTAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(2916..3779)
                     /gene="rsmI"
                     /locus_tag="ISB30_001883"
     CDS             complement(2916..3779)
                     /gene="rsmI"
                     /locus_tag="ISB30_001883"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP1989519.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEDDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            3844..5880
                     /locus_tag="ISB30_001884"
     CDS             3844..5880
                     /locus_tag="ISB30_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAP1989520.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELVNQLPQELNDNQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVNAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTASPDCVINRKLSWLQYQQGQVVPAS"
     gene            5838..6233
                     /locus_tag="ISB30_001885"
     CDS             5838..6233
                     /locus_tag="ISB30_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAP1989521.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFIEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            6253..6843
                     /gene="diaA"
                     /locus_tag="ISB30_001886"
     CDS             6253..6843
                     /gene="diaA"
                     /locus_tag="ISB30_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAP1989522.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            6853..7428
                     /gene="yraP"
                     /locus_tag="ISB30_001887"
     CDS             6853..7428
                     /gene="yraP"
                     /locus_tag="ISB30_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAP1989523.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(7541..8581)
                     /locus_tag="ISB30_001888"
     CDS             complement(7541..8581)
                     /locus_tag="ISB30_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP1989524.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(8654..9289)
                     /locus_tag="ISB30_001889"
     CDS             complement(8654..9289)
                     /locus_tag="ISB30_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP1989525.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNVIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            9417..9935
                     /gene="yhbO"
                     /locus_tag="ISB30_001890"
     CDS             9417..9935
                     /gene="yhbO"
                     /locus_tag="ISB30_001890"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAP1989526.1"
                     /translation="MSKKIAALITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVVIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIVIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(9915..10358)
                     /locus_tag="ISB30_001891"
     CDS             complement(9915..10358)
                     /locus_tag="ISB30_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAP1989527.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            10409..10711
                     /gene="yhbQ"
                     /locus_tag="ISB30_001892"
     CDS             10409..10711
                     /gene="yhbQ"
                     /locus_tag="ISB30_001892"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAP1989528.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVTEGAGFAELLSSLQTPEIKSD"
     gene            complement(10698..11201)
                     /locus_tag="ISB30_001893"
     CDS             complement(10698..11201)
                     /locus_tag="ISB30_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1989529.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLHCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(11195..11719)
                     /locus_tag="ISB30_001894"
     CDS             complement(11195..11719)
                     /locus_tag="ISB30_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAP1989530.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            11928..12923
                     /locus_tag="ISB30_001895"
     CDS             11928..12923
                     /locus_tag="ISB30_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP1989531.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQHAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            12932..13810
                     /locus_tag="ISB30_001896"
     CDS             12932..13810
                     /locus_tag="ISB30_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP1989532.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            13891..14898
                     /locus_tag="ISB30_001897"
     CDS             13891..14898
                     /locus_tag="ISB30_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HAP1989533.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(15016..16260)
                     /gene="mtr"
                     /locus_tag="ISB30_001898"
     CDS             complement(15016..16260)
                     /gene="mtr"
                     /locus_tag="ISB30_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAP1989534.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIVLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(16414..18303)
                     /locus_tag="ISB30_001899"
     CDS             complement(16414..18303)
                     /locus_tag="ISB30_001899"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAP1989535.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(18296..18376)
                     /gene="yrbN"
                     /locus_tag="ISB30_001900"
     CDS             complement(18296..18376)
                     /gene="yrbN"
                     /locus_tag="ISB30_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAP1989536.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(18483..19367)
                     /gene="nlpI"
                     /locus_tag="ISB30_001901"
     CDS             complement(18483..19367)
                     /gene="nlpI"
                     /locus_tag="ISB30_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAP1989537.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(19476..21611)
                     /gene="pnp"
                     /locus_tag="ISB30_001902"
     CDS             complement(19476..21611)
                     /gene="pnp"
                     /locus_tag="ISB30_001902"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP1989538.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKNAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(21858..22127)
                     /gene="rpsO"
                     /locus_tag="ISB30_001903"
     CDS             complement(21858..22127)
                     /gene="rpsO"
                     /locus_tag="ISB30_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP1989539.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(22276..23220)
                     /gene="truB"
                     /locus_tag="ISB30_001904"
     CDS             complement(22276..23220)
                     /gene="truB"
                     /locus_tag="ISB30_001904"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP1989540.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(23220..23621)
                     /gene="rbfA"
                     /locus_tag="ISB30_001905"
     CDS             complement(23220..23621)
                     /gene="rbfA"
                     /locus_tag="ISB30_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP1989541.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(23785..26457)
                     /gene="infB"
                     /locus_tag="ISB30_001906"
     CDS             complement(23785..26457)
                     /gene="infB"
                     /locus_tag="ISB30_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP1989542.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(26482..27969)
                     /gene="nusA"
                     /locus_tag="ISB30_001907"
     CDS             complement(26482..27969)
                     /gene="nusA"
                     /locus_tag="ISB30_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP1989543.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(27997..28449)
                     /gene="rimP"
                     /locus_tag="ISB30_001908"
     CDS             complement(27997..28449)
                     /gene="rimP"
                     /locus_tag="ISB30_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP1989544.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(28656..28732)
                     /locus_tag="ISB30_001909"
     tRNA            complement(28656..28732)
                     /locus_tag="ISB30_001909"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28696..28698),aa:Met,seq:cat)
     gene            29080..30423
                     /gene="argG"
                     /locus_tag="ISB30_001910"
     CDS             29080..30423
                     /gene="argG"
                     /locus_tag="ISB30_001910"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAP1989545.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(30431..>31584)
                     /locus_tag="ISB30_001911"
     CDS             complement(30431..>31584)
                     /locus_tag="ISB30_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAP1989546.1"
                     /translation="CQFAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQR
                     LLSIANTVPYFQLSVRDTGIDTYVLIVGESVRVDNMSLYGYTHSTTPQVEAQRKQIKL
                     FNQAISGAPYTALSVPLSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQ
                     NGTAVTSIAMRAMETVYVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAY
                     PQSSAVFQPQDDQDACYDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKN
                     VYFHGGREASQQAYHVPMFIWYSPVLGDGVDRTTENNIFSTAYNNYIINAWMGVTKPE
                     QPQTLEEVIAHYKGDSRVVDANHDVFDYVMLRKEFTEDNQGNPTPEGQG"
BASE COUNT         7574 a         8562 c         7919 g         7529 t
ORIGIN      
        1 gagcagtcca aatattctct taacccgtat tgataaccgt ctggttcacg gtcaggttgg
       61 cgtgacctgg acatccacca tcggtgcgaa tctgttggta gtcgtggatg atgttgtcgc
      121 taacgatgat attcaacaga aattaatggg tattaccgca gaaacctacg gctttggcat
      181 tcgtttcttt actatcgaaa aaaccattaa cgtcatcggc aaagctgcac cacatcagaa
      241 gatcttcctg atttgccgta cgccacaaac ggtacgtaaa ttggtagaag gtggtattga
      301 cctgaaagat gtcaacgtcg gcaatatgca tttctcggaa gggaaaaagc aaatcagcag
      361 taaagtttat gtcgatgacc aggatctcac ggacttacgt tatattaaac aacgtggcgt
      421 gaatgttttc attcaggacg ttcctggcga tcaaaaagaa caaatccctg actaaatctg
      481 aaatcgcctt aatattggtt tgaggtaata aaaatgcatg aaataaccct actccaggga
      541 ttatccctgg cggcgttagt tttcgttctg gggattgatt tttggctgga agccttattt
      601 ttattccgcc cgataatcgt ttgtaccctt acaggtgcta ttctcggtga tattcagact
      661 ggcttaatta ccggaggcct gacagagttg gctttcgccg gattaacccc tgcaggtggt
      721 gttcagccgc ccaaccctat tatggcgggt ctgatgacca ccgtcattgc atggtctacg
      781 ggcgttgatg ccaaaacggc aattggtctt ggcctgccgt ttagtttgtt aatgcagtac
      841 gtcattctgt tcttctattc cgctttctca ttatttatga ccaaagccga taaatgcgcg
      901 aaagaggcgg atacggcagc gttttcccga cttaactgga caacgatgct catcgtcgct
      961 tcagcgtatg cggtgattgc tttcctctgt acttacctgg cacaaggggc gatgcaggcg
     1021 ctggtgaaag cgatgcccgc ctggctgacc cacggctttg aagtggctgg cggtattctg
     1081 cctgccgttg gttttggctt gctgctgcgc gtgatgttca aagcgcaata tatcccttac
     1141 ctgatcgccg gtttcctgtt tgtttgctac atccaggtca gcaacctgtt gccggttgcc
     1201 gtgctgggcg caggctttgc ggtgtatgag tttttcaatg cgaaatcccg gcagcaagcg
     1261 caaccgcagc ccgttgccag taaaaatgaa gaagaggact acagcaatgg gatctgaaat
     1321 cagtaaaaaa gatatcaccc gtctgggctt tcgttcgtca ctgctacaag caagctttaa
     1381 ctacgaaagg atgcaggcgg gcggttttac ctgggcgatg ttgccgatcc tgaaaaagat
     1441 ttataagaac gacaaaccgg gcctaagcgc ggcgatgaaa gataacctcg aatttattaa
     1501 tacccaccct aatctggtcg gatttctgat ggggttatta atttcgatgg aagaaaaagg
     1561 agaaaaccgc gacaccatta aaggcctcaa agtggcactg tttggcccaa tcgccgggat
     1621 tggcgatgcg attttctggt ttactttgtt gccgattatg gcggggattt gctcatcgtt
     1681 tgccagtcag gggaacctgt tgggaccgat tttgtttttc gccgtttacc tgcttatctt
     1741 tttcctgcgc gtcggctgga cccacgtcgg ttattcagtc ggcgtgaagg ctattgataa
     1801 agtgcgagag aactcgcaga tgattgcccg ttcggcaacc atcctcggga tcacagtaat
     1861 cggcgggctg atcgcttcgt atgtgcatat taacgtggtg acatcgtttg ccatcgacag
     1921 tacccacagc gtcgcgctgc agcaggattt cttcgataaa gtcttcccga atattttacc
     1981 gatggcctac accctgctga tgtattactt cctgcgggtg aaaaaagcgc atccggtgct
     2041 gttaatcggc gtgacgtttg ttctttctat tgtttgttct gcattcggca ttttgtaaat
     2101 ggaacgaggc accgcgtctg gcggtgcctc tttacttaag gaatttcatc ctgtgcaaac
     2161 ccttcaacaa gttgaaaact atacggcgtt aagtgaacgt gccagcgaat atttattggc
     2221 cgtgatccgt agcaaaccgg atgccgtgat ttgcctggcg accggagcca cgccattact
     2281 gacgtatcat tatttggtag aaaaaatcca ccagcagcag gttgatgtca gccagctcac
     2341 cttcgtgaag ctcgacgaat gggtggatct gccattaacg atgcctggta cctgcgaaac
     2401 tttccttcaa cagcatatcg tgcagccgct ggggctacgt gaagaccagc tcatcagctt
     2461 tcactccgaa gagataaatg agacagagtg cgaacgggta acgaacctga ttgcgcgcaa
     2521 aggcggtctg gacttatgcg ttctcggatt ggggaaaaac ggtcatcttg ggctgaacga
     2581 accgggagaa agcctgcaac cggcctgcca tatcagtcaa cttgatgcca gaacacagca
     2641 acatgagatg ttaaaaaccg ctgaccgtcc cgtgactcgt gggatcacct taggcctgaa
     2701 ggatattctc aatgcccgcg aagttttgtt actgataact ggcgaaggaa aacaggatgc
     2761 gacagaacgt tttctcacgg ctaaagtctc caccgctatc ccggcttcat ttttatggct
     2821 gcataaccat ttcacatgcg tgattgatga aatgtgtcgt cgttaactcg cctggtacag
     2881 gcgttgcagc gctattcctg ctgcgacgcc tgtaatcact ctccctgctg ctccagcgca
     2941 tacttataca gcgcattttt cttcacgccg tgaatttctg cggccagcgc cgccgctttt
     3001 ttcagcggca gttctgcctg taacagcgcc agcgtgcgca gggcatcagc aggcaggtca
     3061 tcttcctgcg ctttatggcc ttcgacaatc agcaccattt cgcctttgcg acggttttca
     3121 tcttctttta cccacgccag cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt
     3181 ttggttagct cacgcgccag aaccacgtaa cgggattcgc ctaataccgc aacaatatct
     3241 tccaggctat ctaacagacg gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct
     3301 tcaatagctt ttagcgcatc acggcggcct tttgatttgg caggtaaaaa gccttcataa
     3361 caaaaacgat cggatggtaa acccgcggcg cttaacgcag tgatagcagc acacggccct
     3421 ggtagcggca ccacgcgaat ccccgcttca cgacaggtac gcaccagatg gtagccagga
     3481 tcgttaatta gcggcgttcc ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc
     3541 ttcgccagca gcgtttcggc tttttgttgt tcgttatgat cgtgcagcgc aaacaaccgg
     3601 gcattaatcc caaaatgttg cagcaataaa ccggtgtgac gcgtatcctc ggcggcaatc
     3661 agatcaacgg cctgtaatac ctctaatgca cgctgggtga tatccgccag attgccgatt
     3721 ggcgtcggta caatgtaaag ctgcccctga gaattatccg ccgattggtg ttgtttcatt
     3781 gtgtcgtccg tattgccgat ttaatattga gcattgcgta aaaaaaatat cactggatac
     3841 attatggtac cctcaacatt ttctcgtttg aaagccgcgc gttgtctgcc tgttgttttg
     3901 gcagccctga ttttcgccgg ttgtggcacc catactcccg atcagtccac tgcttatatg
     3961 cagggcacgg cgcaggctga ttctgctttt tacctgcaac agatgcagca aagttctgat
     4021 gataccagga tcaactggca attactcgcc attcgtgcac tggtgaaaga aggtaaaacc
     4081 gggcaggcgg ttgagttggt taaccaacta ccgcaagaac tgaatgataa tcagcgtcgc
     4141 gagaaaacac tgctggcggc agagattaaa ctggcgcaga aagattttgc tggcgcgcaa
     4201 aacttgctgg cgaaaatcac acctgccgat ttagaacaaa accagcaagc ccgttactgg
     4261 caggcaaaaa tcgatgccag ccaggggcgt ccttccattg atttactgcg cgcgttaatt
     4321 gctcaggaac cgctgctcgg tgcgaaagaa aagcagcaga atattgacgc cacatggcag
     4381 gcgctctcct ccatgactca ggagcaggcg aatacgctgg tgatcaacgc cgacgaaaat
     4441 attctgcaag gctggctgga tttgcagcgc gtctggtttg ataaccgtaa cgatcctgac
     4501 atgatgaaag ctgggatcgc cgactggcag aaacgttatc cgaacaaccc gggcgcgaaa
     4561 atgctgccaa cgcagttggt taacgtaaaa gcgtttaaac cagcatcgac caacaaaatc
     4621 gccctgctgt tgccgctgaa tggccaggca gcggtatttg gtcgcactat tcagcaaggc
     4681 tttgaagcgg cgaaaaatat cggcactcag ccagtggcgg cccaggtagc tgccgcacct
     4741 gccgcagacg tagcagaaca acctcagccg caaaccgtgg atggcgttgc cagcccggca
     4801 caagcttcgg ttagcgatct gaccggtgaa cagcctgcgg cccagccggt acctgtaagc
     4861 gccccggcga caagcaccgc agcggtaaac gcacccgcaa atccatccgc agagctgaaa
     4921 atctacgata cctcatcaca accacttagc cagatcttaa gccaggttca gcaggatggt
     4981 gcgagtattg tggtcggtcc gttgctgaaa aataacgttg aagagttgct gaagagcaac
     5041 accccgctga acgtgctggc actgaaccag ccggagaata tcgaaaaccg cgtcaatatt
     5101 tgttacttcg cgctttcgcc agaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac
     5161 cagggtaaac aagcgccgct ggtgctgatc ccgcgcagtt cattgggcga tcgcgtagcc
     5221 aatgcgtttg cgcaagagtg gcagaaactg ggtggcggca ccgttctgca acaaaaattt
     5281 ggttccacca gcgaattacg cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt
     5341 agcccgatta ctcccagagc gacaaccgac tccggcatga cgaccaacaa tccaacgctg
     5401 caaaccacgc caaccgatga ccagttcacc aataatggcg gtcgtgtcga tgcggtgtac
     5461 attgtggcaa cgccgggtga aatcgctttt atcaaaccga tgatcgccat gcgtaacggt
     5521 agccagagcg gtgcaacgct gtacgccagt tcccgcagtg cgcaagggac cgctggcccg
     5581 gatttccgcc tggagatgga aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt
     5641 aatctgccgt taatgcagca ggcgctcagc gcggtgaaca acgattattc gctggctcgc
     5701 atgtatgcga tgggtgtcga tgcctggtcg ttggcaaatc atttctcaca aatgcgccag
     5761 gttcagggtt ttgaaatcaa cggtaatacc ggaagcctga cggcaagccc ggattgcgtg
     5821 attaacagga agttatcatg gctacagtac caacaaggtc aggtagtccc cgccagttaa
     5881 ccaccaaaca gaccggcgat gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag
     5941 gactacggtt tatcgccgct aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc
     6001 gtgaaggccg gaccaccgtt tttatcgagg tgcgttaccg ccgctctgcg ctttatggcg
     6061 gcgcggcagc cagtgtgacc cgcagcaaac aacacaaatt attacagact gcccgtttgt
     6121 ggctcgcgcg tcataatggg agttttgata ctgtggattg ccggttcgat gtggtagcct
     6181 tcaccgggaa tgaggttgag tggattaagg atgcctttaa tgaccactca taattaaggt
     6241 ttaaggatta gcgtgcaaga aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa
     6301 attgcggcgg cagaggcgct tccggatgcc atctcccgtg cagccatgac gctggttcag
     6361 tctctgctca atggcaacaa aatcctctgt tgtggtaatg gaacttccgc tgccaatgca
     6421 cagcattttg ctgccagcat gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc
     6481 attgcactaa atactgataa tgttgtctta acggcaattg ccaacgatcg cttacatgat
     6541 gaagtgtatg caaaacaggt gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt
     6601 tccacccgtg gcaacagccg cgatattgta aaagcggtag aagctgccgt tacgcgtgat
     6661 atgaccattg tggcattaac cggctatgac ggcggtgaac ttgcaggttt gttagggcca
     6721 caggatgtgg agatccgcat tccttcgcat cgtagtgctc gcattcagga aatgcatatg
     6781 ctgacggtaa attgcctgtg cgatctgatc gataacacgc ttttccctca ccaggatgtt
     6841 taaggagaat acatgaaggc attatcgcca atcgcagtcc ttatttccgc gctgctgttg
     6901 caaggttgtg ttgccgctgc cgtagtgggt actgctgctg tgggtaccaa agccgcaact
     6961 gacccacgca gtgtcggcac ccaggtagac gatggtacct tggaagtgcg cgtgaacagc
     7021 gcattgtcga aagacgaaca aattaagaaa gaagcgcgca ttaatgtaac ggcctatcag
     7081 ggcaaagtgc tgctggttgg gcagtcacca aacgctgaac tttcggctcg cgccaaacag
     7141 attgctatgg gcgtagacgg tgccaacgaa gtgtataacg agattcgtca gggccagccg
     7201 attggtctgg gcgaagcgtc taacgatacg tggatcacca ccaaagtacg ttcgcagctg
     7261 ttaaccagcg acctggtgaa atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg
     7321 ttcctgatgg ggctggtgac tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg
     7381 gtaagcggcg tgaagcgggt aactacggcg tttacgttta ttaaatagca gcccttgtaa
     7441 tgcctgatgc gacgcttgca gcgtcttatc aggcctacaa cgatgcaaac cgtaggtcgg
     7501 ataaggcgtt tacgccgcat ccgacatgtt ttccctcaaa tcaaactaac agcgccagcc
     7561 cgccgacaat cacaccagac actgccacca tcgcccccgt cagccataag gctttcgccg
     7621 ggaacgcttt gcgcagcata atcagtgacg gcaaactcac cgccgggagc gtcatcaaca
     7681 atgccagcgc cggagcagtt cccatacctg ccagcatcat cgtttgtaca atcggaattt
     7741 ctgctgccgt gggaatgaca aacaagcatc ctgccaccgc catcgccacc acccacatca
     7801 ggctgttatc gacagcacca tcggcatggg ggaataacca gacgcgagcg gcacccaaca
     7861 ccagtactgc aaggatgtaa accgggatcg tactccagaa aagcgtccat agcgccctgc
     7921 cccagcggct aaaaaatcct ccctgtgctt ccggtatatc aatttcgacc ggtgcctgcg
     7981 tttgcggtgt ttcacgcacc catttttgca ccagcgtcgc aatcagcaac accatcacca
     8041 gccccgccac cagacgaatc gccgcaaaac cccagccgag gacgaacccc ataaacacca
     8101 gcgtcgccgg gtttaacaac ggattcccca tccagaatgc cagcgcaccg cccatcgaca
     8161 cctgttggcg acgcattccc gccgcgaccg gagccgcaca gcaggtacac atcatacccg
     8221 gcagcgaaaa cagcgttccc agcagcgtgc cgcgaaagcg cgattgccca agcgtacgca
     8281 acaaccagtc acgcgggatc agtacctgaa tcaacgaacc gaggatcacc cccagcaccg
     8341 ccgctttcca taccgcgagg aaatagatca tcgcgtaatc caacgccgcc tgccacggat
     8401 tagcatccgc ctgcgcaagg atagatttac cgatactgtg ggtttcggca gcagtaaacg
     8461 ctttgccgta gtaaggttgc catttcacat accagagacc ggcaatgaca acgagaaaga
     8521 aaagcgcggg tttccaccac tgaatgggcg ttgccgcctg agatgaagac tgaccagtca
     8581 tagcattccc caggaataag ttatgataat tgagcgcgtg aatattacgc tcactatcaa
     8641 ttcttgggaa taattattca gctcttttgc gtaattctga agagcttaaa atcgtcacac
     8701 cttcatgctc aggccgcatc gcctctgcca gcatgacccg cgccacgtct ctcgcatcaa
     8761 tggatttcca gttaccgggt aacaaacgga acagcggcgc aaaaagcgtt tcgttcatcc
     8821 gctgtttgct acgatcaccc agtaacatcg acgggcgagc aatggtcaat ttcggccagt
     8881 tctgggcgat taatgcttct tccatctccc ctttgacgcg gttatagaaa aacggcgagt
     8941 gggcattagc acccatagca ctgaccacca gcatatgctg cgcaccgagc cgccgcccgg
     9001 ttaatgcggt atccaccacc aacgtgtaat cggcatgaat aaacgcctct ttgctccccg
     9061 cttctcgccg cgtggtgccg agacaacaaa acacaatgtc gatgggatcg gtgacctgcg
     9121 ccagcgcgtc agtcagttgc ggatcatggg gattaaacac cccgggcata tcgcccaacg
     9181 gacgtcgcgt cggcgcagca atgacgttaa ctttcggttc attaatcaac atccgcagca
     9241 ggtgaccgcc caccagcccc gttgcgcctg taatcagtac ctgactcatc ttcgctcctt
     9301 tacagaattg tctgccttgc gctccccagc tcatgcacca ggcttaatag accatgaggt
     9361 aattatcccc gattgtggaa aattcgcctc atccaatgca acaacgcgga ggaagcatga
     9421 gtaagaaaat cgccgcttta atcactgatg aatttgagga ttcagaattc acttcacccg
     9481 cagacgagtt ccgtaaagcc ggacacgaag tgattaccat tgaaaaacaa gcgggtaaaa
     9541 cggtgaaagg caaaaaagga gaagccagcg tggtcatcga taaatccatc gatgaagtga
     9601 cgcccgcaga gttcgatgcc ctgctgctac cgggcggtca ttcaccagat tatctgcgtg
     9661 gcgacaaccg ttttgtcacc ttcactcgcg attttgtgaa cagcggcaaa cctgttttcg
     9721 ccatctgtca cggtccccag ttgctgatca gcgccgatgt gatccgtggt cgtaagctga
     9781 ccgccgttaa accgatcgtg attgatgtta aaaacgcggg cgcggaattt tacgatcagg
     9841 aagtggtggt cgataaagat cagctggtta ccagccggac accggacgat ctgccagcgt
     9901 ttaaccgcga ggcgttacgc ctgctcggtg cctgagttac gtagccaaat cattttttta
     9961 ccaaagccca gcgtgttgtc ggtgaatttg atttcatcga gccggatttc ccataccggg
    10021 gccgacagca ttctggcaac cggaaagcga cgattgtacg ccttgcgtgc gaggtcgctt
    10081 tcctcgcctt ccagtctgcg gatctcacct ttaaactgca cgccgcgaat taacgctacc
    10141 gttttcggct gaccgtttac cgttccggca attgccgcct gcggcccgct catctgcgca
    10201 tgacgcgttt tttcttccgt caaaatgtag aaggcgactt tctgcgcatc aaaaagataa
    10261 aaggcattgg cgcaccacag ttccccttcc tgctgcacac accaggtgac aacatgttgt
    10321 tttgccagcc aacggctgat ggcggtgagt gtttccattg ctgttctctt ttatactgtg
    10381 ggcctgacct taacatgcga cgcagacgat gacaccctgg tttctttact tgatccgtac
    10441 cgccgacaat aagctttata ccgggatcac cacggatgtc gaacgccgct atcagcagca
    10501 ccaaagcggc aaaggggcga aagcactgcg agggaaagga gaactaacgc tggcgttttc
    10561 cgcgccagtg ggcgaccgtt cgctggcact acgagcggaa tatcgcgtta aacaactgac
    10621 taaacggcaa aaagagcgtc tggtaacgga aggcgcaggg tttgcagaac tgttgagcag
    10681 cctgcaaacc ccggagatta aaagcgattg aaatgctcgt gatactcgac cagaccagtt
    10741 acgccgttca gcgcgtcatc cgctaaacga tgtacctgga aggcgctttc agtgcccggc
    10801 cagcggcaat gcagatcgtg atgcgccgcc agttcaaagc cgaaacggct gtacagcgcc
    10861 ggatcgccca gcgtcaccac tgcggcatag ccgaactcat taagcgaatc gagtccttca
    10921 tagaccagtt ggcgtgccag cccctgtcca cggtattttt catcaaccgc cagcggtgcc
    10981 atgccgaccc attgcaggtc ttcgccctgc acatcaaccg gactaaaagc cacatagcca
    11041 atgacctgac cttcgtcatc tgtcgccacc agccccagcg tcagaaagcc atcttcacgc
    11101 agatcgtgaa ccagcttcgc ttccgcatca ctttcgaatg aacgacgcag cagggcatca
    11161 atacccggcg catcaatggg aatttctact cgaattagca tggttcacct accgatgtct
    11221 gtttggtttc aggcgcggtt ttcattcccg cctcaacaaa atccgccagt tgcagcagca
    11281 tcatgcgtaa ggctttcggc atttgctcca gttcaatggc gtccatcagg tttttcacat
    11341 acagccccag ctccgtatcg ccttcaatca ccagccgacg ctggaagaag agcgtatccg
    11401 gatcttgttt acgcgccgca atcatcagca gatcgctggc gtcggcacta aaactcacat
    11461 cagcttgcgc gttctggcta acgaccagtt tgccattcac caccgaggta aaccattgca
    11521 ggtcaatatc acgcacatga atacttaacc agcgaccttc aagaaactcc agctcgccat
    11581 catccagcgc ctgacggaat tgccagctta agacctgctc aagaacctgg cgttttagcg
    11641 caaatggcgt cagttttacc ggtacactca acagagatgg ccccaaatgc acaatacggg
    11701 aacgcagttt atccaacacg agttttactc cctgtttcaa caatcatcct attttgccat
    11761 atcagaaaaa taacatagcg gtataaatca acaattccat atgaaattgc tgctaccacc
    11821 aatacaactt taactgcctt aaatcaaaaa ttgtcgcagc aaggttaact aaaatcccag
    11881 ttcgttaaca tttttgcgtt ttgatagcgc aaccttcagg aaaaattatg gagctgctct
    11941 gccctgccgg aaatctcccg gcgcttaagg cggccatcga aaacggcgca gatgctgttt
    12001 atatcgggct aaaagatgat accaatgccc gtcacttcgc cggccttaac tttaccgaga
    12061 aaaaattgca ggaagcggtg agttttgtcc atcaacatcg ccgcaaactt cacatcgcga
    12121 ttaacacttt tgcgcatccg gacggttacg cccgttggca gcacgccgtg gatatggcgg
    12181 cgcaactggg tgccgacgcg ctgatcctcg ccgacctcgc catgctggag tacgccgccg
    12241 aacgttatcc gcatattgag cgccacgtat cggtgcaggc ttcggcgacc aatgaagagg
    12301 cgattaactt ttatcatcgc cattttgacg ttgcccgcgt ggtactgccg cgcgtgttgt
    12361 cgattcatca ggtgaaacag ctggcacggg tcacacctgt accactggaa gtgtttgctt
    12421 tcggcagcct gtgcattatg tcggaaggcc gttgctatct gtcgtcgtat ctgacgggtg
    12481 aatcgcccaa caccgtgggg gcatgttctc cggcccgttt cgtgcgctgg cagcaaacgc
    12541 cgcaagggct ggaatcccgc ctgaacgaag tgctgatcga ccgttatcag gacggcgaaa
    12601 acgcaggtta tccgacgctg tgtaaagggc gttatctggt ggacggcgag cgctatcacg
    12661 cgctggaaga accaaccagt ctcaataccc tggaactgct gccggagtta atggcggcga
    12721 atattgcttc ggtgaaaatt gaaggccgcc agcgtagccc ggcgtatgtc agccaggtgg
    12781 cgaaagtctg gcgtcaggct atcgaccgtt gtaaggccga tccgcaaaac ttcgtaccgc
    12841 aaagcgcgtg gatggagacg ctcgggtcga tgtccgaagg cacgcaaact acccttggcg
    12901 cgtatcaccg taaatggcag tgagaaaagc aatgaaatat tccttagggc cggtgctatg
    12961 gtactggcca aaagagacgc tggaagactt ttatcagcag gccgccgcca gcagcgcaga
    13021 cgtgatttat cttggtgaag cggtatgcag caagcgccgg gcaaccaaag ttggcgactg
    13081 gctggagatg gcaaaatcac tcgccggaag tggtaagcag attgtgctct ccacgctggc
    13141 gctggtgcag gcatcctctg aactgggcga actgaaacgc tatgttgaga acggtgagtt
    13201 tctgattgaa gccagcgatc tcggcgtggt gaatatgtgc gctgaacgca aactgccgtt
    13261 cgtcgccggg cacgcgctga actgctacaa cgcggtgacg ctgaaaatat tgcttaaaca
    13321 gggcatgatg cgctggtgta tgccggtgga gctttcccgc gactggctgg tgaatctgct
    13381 taatcagtgc gatgagttgg gcattcgcaa ccagtttgaa gtggaagtcc tgagctacgg
    13441 tcatctaccg ctggcctact ccgcacgctg ctttaccgcg cgttcggaag accgcccgaa
    13501 agatgagtgt gaaacctgct gcattaagta tccgaacggg cgcaacgtac tgtcgcagga
    13561 aaaccaacaa gtgtttgtac tcaacggcat tcagaccatg agcggctacg tttacaacct
    13621 cggtaacgag ctggcatcca tgcaggggct ggtcgatgtg gtccgcctgt caccgcaggg
    13681 tactgacact ttcgcaatgc tcgatgcctt ccgcgctaat gaaaatggcg ctgcgccact
    13741 accgttgacg gcaaacagcg actgtaacgg ctactggcgg cggctggccg ggctggagct
    13801 gcaagcgtaa gtatatagct cactttgtta acaactttaa ctactcttta atgcagtatt
    13861 aaagactaat cggtaacaaa gtgagctgtt atgactgata aaaccattgc gttttcgcta
    13921 ctcgatctgg cccccatccc cgaaggttct tcagcgcggg aagcgttctc ccactctctc
    13981 gacctcgccc gtctggctga aaagcgcggc tatcatcgct actggctggc agaacaccac
    14041 aatatgaccg gcattgccag tgctgcaacg tcggtgttaa ttggctatct ggcggcgaat
    14101 accaccacgc tgcatctggg gtctggcggc gtgatgttgc ctaaccactc accgttggtc
    14161 attgccgaac agttcggcac gcttaatacg ctctatccgg ggcgaatcga tttggggctg
    14221 ggtcgcgcgc cgggtagcga ccaacggaca atgatggcgc tacgtcgtca tatgagcggc
    14281 gatattgata atttccctcg cgatgtcgcg gaactggtgg actggtttga tgcccgcgat
    14341 cccaatccgc atgtgcgccc tgtaccgggc tatggcgaga aaatcccggt gtggttgcta
    14401 ggctccagcc tttacagtgc acaactggcg gcgcagcttg gtctgccgtt cgcgtttgcc
    14461 tcacacttcg cgccggatat gctgttccag gcgctgcatc tttatcgcag caacttcaaa
    14521 ccgtcggcac ggctggagaa accgtatgcg atggtgtgca tcaatattat tgccgccgac
    14581 agtaaccgcg acgctgagtt tctgtttacc tcaatgcagc aagcctttgt gaagctgcgt
    14641 cgcggcgaaa ccgggcaact gccgccaccg attcaaaata tggatcagtt ctggtcaccg
    14701 tccgaacagt atggcgtgca gcaggcgcta agtatgtcgc tggtgggcga taaagcgaaa
    14761 gtgcgtcatg gcttgcagtc gatcctgcgc gaaaccgacg ccgatgagat tatggttaac
    14821 gggcagattt tcgaccacca ggcacgcctg cattcgtttg agctggcgat ggatgtgaag
    14881 gaagagttgt tggggtagtg tgttttaacg taggaagcct tatccgagat ggcaacgctg
    14941 tcctacgtag gcctgataag cgaagcgcat caggcattgt gtaggcagca gaaatgtcgg
    15001 ataaggcacc tctgattact gatacaccgg cagcacatta aagctcgata agatatgcac
    15061 cagtgcgttg ccgacgccaa acaccagaat cagcacaatc atcggcttgc cgccccatac
    15121 acggaatttc gggctaccaa agcgtttacg cgatgcgcgg gccagcagcg ccggaacaat
    15181 tgccgcccag atggtagccg ctaagccagc ataaccaatg gcgtacagga atccgttagg
    15241 aaacagcagg ccacccacaa ccggcggggc aaaggtcagc aacgctgttt tcaaacggcc
    15301 cacagccgag tcgtcgaaac caaacagatc tgccagatag tcaaacaggc ccagcgttac
    15361 gccaaggaac gaactcgcta ccgcaaagtt tgagaacaca accagcagaa ggtccaggct
    15421 acggctgttc agtacgccgc ttaacgcctg taccagcaca tcaatattac cgcccttctc
    15481 ggcgatgccg ataaactccg gacgcgggat gttacccatc gtcgccagca accagatggt
    15541 atacagcgcc agcgccatca gcgtaccgta caccaggcat ttcacgatgg ttttcggatc
    15601 tttgccgtaa tacttcatca ggctcggtac gttaccgtga taaccaaacg atgccagaca
    15661 gaacggcagc gtcatcaaca gatacggtgc ataagacgca ttgctttcgg cgacgttgaa
    15721 caatgtcgca ggctgcacat gccccagcag gctaccgaag gtgaggaaga aggtaatgac
    15781 tttcgccccc agcacaatcg ctgtcatgcg actgacggct ttggtgctca accacaccac
    15841 aaacgccacc aacagtgcaa aaccaaaacc cgctgcccgt gccgggacgt ttagcgacat
    15901 ctctgcgaag gtgtgatgca gaatcgaacc actggcagaa atataggcat aggtcaggat
    15961 gtagagcaca aaggcaatgg aaatgccgtt gaccacgttc cagcctttgc cgagcaggtc
    16021 tttggtgatg gtgtcaaagc tcgaaccgat tcggtaattc aggttagctt ccagaatcat
    16081 caagccggaa tgcagcatac agaaccaggt gaaaatcagc gccgccattg accagaagaa
    16141 ccacgccccg gacatgacca ctggcagaga aaacatccct gcgccaataa tggtgccgcc
    16201 gataatcacc acgccgccaa gcagcgacgg tgacgtttgg gtggtggtta gtgttgccat
    16261 gagggcttct ctccagtgaa aaatagtgcg actgcgttgt tatgcattgc actgtaccag
    16321 tacatgagta caaaaaacag aaaaaaagcc ccgatgggta aaatcggggc tgtatatatt
    16381 attttacaga ttgtgttcgc tgttcagcga tgattacgca tcaccaccga aacgacgacg
    16441 accggtagag tcatcacgac gcggtgcgcg gccttcacga cgttcgccgc taaaacgacg
    16501 cccatcacca cggccacctt cacggcgttc accgctgaag ttacgaccgc cttcacgacg
    16561 ttcgccaccg aaaccacgac caccgccacg acgctcaccg ccagtatgcg gctgtgcatc
    16621 gcccagtaac tgcatgttca ttggcttgtt gagaatgcga gtacgcgtaa agtgttgcag
    16681 cacttcaccc ggcatacctt tcggcagttc gatggtggag tgagaagcaa acagcttgat
    16741 gttaccgatg taacggctgc tgatgtcgcc ttcgttagca atcgcgccaa cgatatgacg
    16801 aacttcaaca ccatcatcgc ggcccacttc aatacggtac aactgcatat cgccaacatc
    16861 acgacgttcg cgacgcggac gatcttcacg gtcgccacgc gggccacggt cgttacgatc
    16921 gcgcggacca cggtcatcac ggtcacggaa ttcacgtttc ggacgcatcg gcgcatctgg
    16981 cggaacaatc agagtacgtt caccctgtgc cattttcagt agtgcggctg ccagagtttc
    17041 gagatccagc tcttcacctt cggcagtcgg ctgaattttg ctcagcagtg cacggtattg
    17101 atccagatcg ctgctttcca gctgctgctg tactttagcg gcaaattttt ccagacgacg
    17161 tttgcccagc agttctgcgt tcggcagttc tacttccgga atggtcagct tcatggtgcg
    17221 ttcgatgttg cgcagcagac gacgctcacg gttctcaacg aacagcagtg cacgaccagc
    17281 acgacctgca cgaccggtac gaccgatacg gtgaacgtaa gactcagaat ccatcgggat
    17341 atcgtagtta accaccaggc tgatacgctc aacgtccagg ccacgggctg caacgtcggt
    17401 cgcaatcagg atgtccagac gaccatcttt caggcgttcc agtgtctgct cacgcagcgc
    17461 ctggttcatg tcaccgttca gcgcggcgct gttgtagccg ttacgctcaa gagcttctgc
    17521 cacttccaga gtcgcgtttt tggtacgaac gaagataatc gccgcatcaa aatcttccgc
    17581 ttccaggaaa cgtaccagtg cttcgttttt acgcataccc cagacagtcc agtagctctg
    17641 gctgatgtca ggacgggtag tcacgctgga ctgaatgcgc acttcctgcg gctctttcat
    17701 aaagcggcgg gtaatgcgac gaatcgcttc cggcatggtt gcagagaaca gagcggtctg
    17761 atgaccttcc gggatctgcg ccataatggt ttcaacgtct tcgatgaagc ccatgcgcag
    17821 catttcgtca gcttcatcca gaaccagacc gctcagttta gagaggtcca gagtgccacg
    17881 tttcaggtgg tccagcagac ggcccggtgt accgacaacg atctgcggcc cctgacgcag
    17941 ggcgcgtaat tgcacgtcat aacgttggcc gccgtacagg gcaaccacgt ttacgccgcg
    18001 catgtgttta gagaaatccg tcattgcttc agcaacctgt accgccagtt cgcgggtcgg
    18061 tgccagcacc agaatctgtg gtgctttcag ctcaggatca agattctgca gcagaggtaa
    18121 agagaatgct gcagtttttc cgctccccgt ctgggccata cccagaacgt cgcggccatt
    18181 cagcagatgt ggaatacact ctgcctgaat tggagatggt ttttcgtaac ccagatcgtt
    18241 aagggcttca aggataggag ccttcaggcc cagatctgca aaagtggttt cgaattcagc
    18301 catgtagtac gtgtgcctca aaattaatgg cggccagtct acataactca tcatgaaatt
    18361 gatcagcaat tttcattgaa aagtgtgaac cggctcaaag taggtgtatt aacgaacaac
    18421 aacgccctca cccgttaagg tgatggcaat caaaaaagat tacgggctga tgtgtacgtc
    18481 agctattgct ggtccgattc tgccaggtca tcttggtcct ggcccaggag cgataattcc
    18541 aacaatgcgt atcggtgctc aacaaagtta tgaacgttgt tggcaaccgc cagtttgaac
    18601 agtgccgtgg cgctgtccaa atcccccaga cttaggtagt acttacctaa atagaagttg
    18661 gtttcactga gatgctcagc gagcgaggtg ttatccgttg cgtccgcctt gagcctttcc
    18721 attaacgttt gttcgctaat gttgcccagg tagaactcga caatgttcca tccccactgt
    18781 tccttatccg atttttcgaa gtgctgtttc aacacttctt tagcctgctt ctcatcgagc
    18841 ttctgctcgg cgagataaag ccacagacta cggaaaggat cattgggatc gtcttgataa
    18901 aacgccagca gatcatcttg cgctaacttg tcacgaccgc cgtaatataa tgcgatcccg
    18961 cgattcaagt gcgcgtagtt gtaagttgga tcaagctcaa gtacagaatc aaacgcttca
    19021 taggcagcat caaaattgcc tgcctgcgtt aaatatatgc ctaagtaatt gaatacttca
    19081 ggcatatccg gtcggattgc cagcgcttgc gaaaaatcgt tacgcgctaa tgccctcaga
    19141 ccgagactat catacaacac tccgcgctca tataaaagct gtgcgcgttc gtcatcggtt
    19201 aaagcccgac tggcaaggat ttgttccata cgtgccagaa tcacttcctg ctgtaaagtc
    19261 ggttgcaatg gtaccgcgag gacttcactt ttacgccagg aagtattact gcatcctgca
    19321 agcgtaagtg ctgtcgcaac gaaacaccag cgcaaaaaag gcttcatttc ccactcccga
    19381 agaccacggt tgaatgaacg tcctgttccc ggttgctaac aaggcgtcct gcccggttaa
    19441 aagccccccg ccgcagcgga gggcaaatgg caaccttact cgccctgttc agcagccgga
    19501 gcttccggtg ctgcagcagg ttgagactgc tcagtcgctt ctttaatgct cagacggata
    19561 cggccctggc gatcaacttc cagaactttc accggtactt cctgacccat ctgcaggtaa
    19621 tcggtcactt tctcaacgcg tttatcagcg atttgagaga tgtggaccag accttcttta
    19681 ccgccgccga tggcaacaaa tgcgccaaag tcaacgattc gggtcacttt accattgtag
    19741 acgcggccta cttcgatttc tgcagtgatc tcttcgatac gacgaatagc gtttttcgcc
    19801 ttctcgccgt cggtcgctgc gatcttcaca gtaccgtcat cttcgatttc gatggtagtg
    19861 ccagtttctt cggtcagagc acggattaca gaaccgcctt taccgataac gtctttgatc
    19921 ttgtctggat tgatcttgat ggtatggata cgcggagcga actcagagat atcaccgcgc
    19981 ggcgcattga tcgcctgttc cattacgccc aggatatgca gacgcgcacc tttagcctgg
    20041 ttcagtgcaa cctgcatgat ctctttggtg ataccttcaa ttttgatatc catctgcaat
    20101 gcagagatac cgtcacgaga acccgcaact ttgaagtcca tatcgcccag gtgatcttcg
    20161 tcgcccaaaa tgtcagacag tacaacgtag ttgtcgcctt ctttcaccag acccattgcg
    20221 atacccgcaa cggcagcttt gatcggcaca ccggcgtcca tcagcgccag agacgcgccg
    20281 cacacggaag ccatagaaga ggaaccgttg gattcggtga tttcagacac aacacgtacg
    20341 gtgtacggga atttgtccat atccggcatt actgccagca cgccgcgctt cgccagacga
    20401 ccgtgaccaa tttcacgacg cttcggagaa ccgaccatgc cggtttcgcc tacggagtac
    20461 ggagggaagt tgtagtggaa caggaaggta tcggtacgtt cgcccatcag ttcatcaaga
    20521 acctgcgcgt cacgagcagt acccagcgtt gcggtaacca gcgcctgcgt ttcaccacgg
    20581 gtgaacagcg cagaaccgtg agtacgcggc agcacaccag tacgcacatc cagaccacgg
    20641 atcatatctt tttcacgacc gtcgatacgc ggttcgccgg ccagtacgcg gctacgaaca
    20701 acatttttct cgatagcgtg cagaatttca cccagttcgt tttcgtccag ggtttcgtct
    20761 tcagcaagca gtgtcgcgat ggtttcagat ttgatgacat caacctgcgc ataacgctct
    20821 tgtttgtcgg tgatgcggta agcatcgctc agacgagctt cagccagcgc agcaacgcgc
    20881 gcgttcagcg cttcgtttac cggctccggc tgccaatccc aacgcggttt accggcttct
    20941 ttcaccagtt cattgatgtt ctgaataaca acctgctgtt gttcatgacc gaacaccact
    21001 gcgcccagca tttggtcttc gctcagcagt tcagcttcag attcaaccat cagcacggca
    21061 gcttctgtac cggcaacaac caggtccagt ttgctctctt tcagttcgtc ctgagtcggg
    21121 ttcagtacgt actggtcatt gatgtaacct acacgggcag caccaatcgg gccattgaac
    21181 ggaataccag acagagacag tgctgcggaa gcaccaatca tcgcgacgat atccgggtta
    21241 acctgcgggt taacagaaac cacggtggcg ataacctgaa cttcgttgac gaagccttcc
    21301 gggaacagcg ggcgaatcgg gcggtcaatc agacgcgcga tcagggtttc gccttcgctt
    21361 gggcggcctt cacgacggaa gaagctaccc gggatacgac cagcagcgta ggtacgctcc
    21421 tgatagttaa cggtcagcgg gaagaagtcc tgacctggtt tggctttttt ctgaccaaca
    21481 acggtaacaa ataccgcggt gtcatccatg ctaaccataa cagcggcagt agcctgacga
    21541 gccatcatgc cggtttccag agtcacggtg tgttggccgt actggaattt acgaacgatc
    21601 ggattaagca atgtaatatc ctttctcttt cttagacagt accttacggt actggtgtta
    21661 atacccgatc ttctgcgcat cctcgcgact aatgacaacc ctaacccagc tctatgtggg
    21721 taaagcctct cattagccgc gcgaacctct gcaacggaag atcattcata gcaacaatac
    21781 attagtttcc agtgaattgc tgccgtcagc ttgaaaaaag gggccactca ggcccccttt
    21841 tctgaaactc gcaagaatta gcgacgcaga cccaggcgct cgatgagctg ggtgtaacgt
    21901 gctacgtctt tacgtttcag gtagtcgagc agtttacgac gctgagaaac catgcgcagc
    21961 agaccacgac ggctgtggtg atcttttttg tgctctgcaa agtggccctg caggtggttg
    22021 atctgtgcag tcagcagtgc tacctgaact tcggtagaac cggtgtcgtt tgcgtcacga
    22081 ccaaactcag aaacgatttt agctgttgct tcagtactta gagacatttt aaaactccaa
    22141 agtatataga atgaaaggac gccgatctct aattcagcga tcccagtgta cgttacgcaa
    22201 agtgttaaac aatttacgcg acgttaagcg gcagtattct actcgtagcg acctgttatc
    22261 gcaagacggt taacattacg ccgggtattc aaccaccagg cgacgaggcg caacgcggcc
    22321 ttcatcgtca atttcgccca taccgataaa cttgccgttc tcaccctctg taacgcgaac
    22381 cagtccttcc agcggcgcac cggatgtacg aactgggtta ccatttttga agtaaacaga
    22441 agacgttaac ggaagattca ccactgggta gtccgaagct ggactgtcca ttggcatcag
    22501 taatgggtca agtaactccg cagccggaat atcctgctgt tcggcttgct caaccagttc
    22561 acgcagatgt tccagggtca ccatccgttc aaccggatat ttacttaccg ccagacgacg
    22621 caagtaaata acatgcgcgc cacaaccgag tttttcgccg agatcgtcaa tgatagtgcg
    22681 gatataagtg ccttttgagc agtgaatttc cagctccagc tcattgcctt catggcgaat
    22741 aaacagcaat tcataaacgg taatcgggcg cgcttcacgt ggaacttcaa tgccctgacg
    22801 cgcgtattcg tacagttttt tgccctgata tttcagtgct gaatacatcg aaggaatctg
    22861 ttcgatatcg ccacggaagg tatccagcgc cgccgccagt tgctctgcac taaaggttac
    22921 cggacgttct tcaacgattt gtccgtcggc atcagaagta tcggtacgct gtccaagacg
    22981 cgcaatgacc cgataacgtt tgtcggagtc cagcagatac tgggaaaact tcgtcgcttc
    23041 cccgaggcaa attggcaaca tgccggtcgc cagcgggtcc agcgcaccgg tatgcccggc
    23101 acggttggcg ttataaatcc gtttcacttt ttgcagcgca tcgttgctgg acatcccctg
    23161 cggtttatcc agcaacaaaa cgccgttaat gtcgcgaccg cgacgacgag gacgactcat
    23221 tagtcctcct tgctgtcgtc cgggttaaca cgacgttctt cgtcatgttt gaccacgctg
    23281 gtcaccaggt ttgacatgcg catcccttca accagagagt tgtcgtagaa gaaggtcagt
    23341 tccggcacga tacgcaggcg catcgctttc cccagcaggc tgcggatgaa accagaagct
    23401 tcctgcaacg ctttgatgcc cgctttaacc gcgtcttcat ctttgtcgtt gaggaacgtc
    23461 acatatactt tggcatacgc caggtcgcga gacatttcga caccggaaac ggtggtcatc
    23521 atgcccagac gaggatcttt aatttcacgc tgcaggatga gagcgatctc tttttgcatt
    23581 tcctgcgcta cgcgctgcgg gcgaccaaat tctttcgcca taataaattc tcctgacaaa
    23641 aaaggggctg ttagcccctt tttaaaatta atttcaggtg gaagggctgt tcacgttggc
    23701 ctgataagac gcgccagcgt cgcatcaggc aatccataca ggatgcggca taaacgcctt
    23761 atcccgcatg gaaccctaaa aaccttaagc gatggtacgt tggatctcga tgatttcgaa
    23821 tacttcgatc acatcgccag tgcggacatc gttgtagttc ttaacgccga taccacattc
    23881 cataccgtta cggacttcgt taacgtcatc tttgaagcgt cgcagggact ccagctcgcc
    23941 ttcgtagata accacgttgt cgcgcagaac gcggatcggg ttgtgacgtt taaccacacc
    24001 ttcggtaacc atacaacctg cgatagcacc aaatttcggt gatttgaaca cgtcacgaac
    24061 ttccgccaga ccgataatct gctgtttcag ttccggagac agcataccgc tcatcgccgc
    24121 tttcacttcg tcgatcaggt tatagatgac ggagtagtaa cgcagatcca ggctttccgc
    24181 ttcaatcact ttacgtgcag aggcatcagc acgtacgttg aagccaacca ggatggcgtt
    24241 ggacgccgca gccagggtag cgtcggtttc ggtgatacca cctacgccag aaccgatgat
    24301 cttcacttta acttcgtcag tagacagttt cagcaaggag tcagaaattg cttccacaga
    24361 accctgtacg tctgccttca ggacgatatt cacttcgtga acttcgcctt cggtcatgtt
    24421 ggcgaacatg ttctcgagtt tagatttctg ctgacgcgcc agtttaactt cgcggaattt
    24481 accctgacga taaagtgcaa cttcacgcgc tttcttctcg tcacgtacaa cggtaacttc
    24541 atcacccgca gccggtacgc cggacaggcc gaggatttcc accggaatgg acggacccgc
    24601 ttccagcact tcctgaccca gttcgttacg cattgcacga acacgaccgt attcgaagcc
    24661 acacagaacg atatcgccct tgtgcagagt accttcacgt accagaacgg tagcaaccgg
    24721 accacgacct ttatcgagga aggattcgat aaccgcaccg ctcgccatac ctttacgtac
    24781 cgctttcagc tccagaactt ccgcctgcag caggatagca tccagcagtt catcaatacc
    24841 ggtacccgct tttgcagata cgtgtacgaa ctggctttcg ccgccccact cttctggcag
    24901 gatgccgtac tgggagagtt cgttcttaac gcgatccgga tcggcttctg gtttatcgat
    24961 tttgttcaca gcaaccacca ccggaacacc cgccgctttc gcgtgctgga tagcttcgat
    25021 ggtctgcggc attacaccgt cgtctgcagc aacaaccagg actacgatgt ccgttgcctg
    25081 cgcaccacgt gcacgcattg aagtaaacgc ggcgtgcccc ggggtgtcca ggaaggtgat
    25141 catgccgttt tcagtttcaa cgtggtatgc accaatgtgc tgggtaatgc cgcccgcttc
    25201 gccagaggcc actttcgttg aacgaatgta gtccagcaga gaggttttac cgtggtcaac
    25261 gtgacccatg atggtcacaa ccggcgcgcg cggttcagcc gcagcacccg tgtcacggtc
    25321 gctcattacc gcctcttcca gctcgttttc acgacgcagg ataactttgt ggcccatctc
    25381 ttcagcaacc agctgtgcgg tttcctgatc gataacctgg ttgatggttg ccattgcgcc
    25441 cagtttcatc atcgctttga tgacctgaga gcctttaacc gccatcttgt tcgccagttc
    25501 gccaacggtg atggtttcgc cgatcacaac gtcacggtta acggcctgag ctggcttctg
    25561 gaagccttgc tgcagcgaag aacctttacg ttttccgccc ttaccgccac gtactgctgc
    25621 acgagcttct tcacgatcag cttttgattc agcgtgtttg ttgcctttct tcggacgcgc
    25681 tgctttcgcg ttacgaccac ggccacggcc gccttcgact tcacgatcgc tttcgtcttc
    25741 tgcctggcga gcatgttgag aagtagtgac gtgataatcg ctggaatctt cagtcggttc
    25801 cgcgttatca gtccatttgt tttcttccgc catacgacgt gcttcttcag caacgcgacg
    25861 tgcttcttct tcgagtttac gacgcgcttc ttcttcggct ttacgcttga gctctgcagc
    25921 ttcctgctca cggcgggctt tttcagcctg ggcgttttta gtcatatcgt cttgttgatt
    25981 gctcactttg tctttttccg cagcttcacg tttcgcttgt tcagcagctt cacgcttagc
    26041 ttgttctgcg gcctcacgtt cagctttttg ttgcgcctcg cgtttagccg attcttctgc
    26101 ctcacgacgg gcttgctctt ccgcttcacg ctgcgcttgc tcttccgctg caaggcgctc
    26161 agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg cggacttcga tttgtaccga
    26221 tttgcttttt ccaccggtac caggaatgtt aagggtgctg cgtgttttac gttgcagcgt
    26281 caatttgtcc gggcctgaat ttttctgatt caggtggtca atcaaagtct gtttctcttg
    26341 tgcagacaca gagtcgtcag cagacttccg gatacctgca tcagcaaatt gctgtaccag
    26401 gcgttccacg gaggtctgtc gctctgcggc cagcgtttta atcgttacat ctgtcatgct
    26461 gttccttcct gctacagttt attacgcttc gtcaccgaac cagcaaatat tacgggcagc
    26521 cataatcagt gctccggctt tttcgtcggt caacccttcg atatcagcca gatcatcaat
    26581 gccctgttcg gcgagatctt ccagcgtaca aacgccacgg gcggccagtt tgaatgccaa
    26641 atcacgatct accccttcaa ggttcagcag atcatcagcc ggtttgttat caccgaggct
    26701 ttcttcctgg gcctgtgcaa tggtggccag tgcattttta gcacgctcgc gcagtgcttc
    26761 aacggtcggc tcatcaaggc cttcgatttc caacagctct ttcatcggca cataggccag
    26821 ttcttccagc gtcgagaagc cttcttctac cagaacagtc gcgaagtctt catcgatgtc
    26881 gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct tagcctgcag
    26941 gtcgtcaacg gtcatcacgt tgagttccca accgctcagc tgcgaagcca gacgcacgtt
    27001 ctgaccgtta cggccaatcg cctgcgccag gttaccggct tcaacggcga tatccatggt
    27061 gtgtttatct tcatccacca cgatagaagc aacgtctgcc ggtgccattg cgttaatcac
    27121 gaactgcgcc gggttatcat cccacaggac gatatcgata cgctctccgc ccagttcagt
    27181 agaaaccgcc tgaacacgcg cgccacgcat acctacgcaa gcacctaccg gatcgatacg
    27241 tttatcgttg gttttcaccg caattttcgc acgagatccc gggtcgcgag ccgctgcttt
    27301 aatttcaatc acttcttcgc cgatttccgg cacttcaata cggaacagtt cgatcagcat
    27361 ttccggcttg gagcgggtga cgaacagctg cgcgccacgc gcttccgggc gaacggaata
    27421 gagcacgcca cgaacgcggt cgccagggcg gaagttttca cgcggcagca tatcttcgcg
    27481 caggatcacg gcttcagcgt tgttgcccag atccagagag atgttgtcgc ggtttacttt
    27541 tttcaccacg ccggtgatga tttcaccttc gtgttcacgg aactgatcaa ccaccatcgc
    27601 acgttcggct tcacgcactt tctgcacgat aacctgtttt gccgtctggg tagtgatacg
    27661 atcaaaggta acagactcaa tctgatcttc aacgtaatcg cccaagttca ggctttcatc
    27721 ttcataacgt gcggcttcaa gggtgatttc cttggtcggc tgggtgactt catcaacaac
    27781 taaccagcga cggaaggtgt caaaatcacc gcttttgcga tcgatctgta cgcgaacgtc
    27841 gatttcttgt tcatattttt tctttgttgc tgtcgccagc gcgctttcca atgcttcgaa
    27901 aatcttctcg cgaggtagcg ccttttcatt ggatacggct tcaactacag ccaaaatttc
    27961 tttgttcatc gcgggctttt cacctcatcc agactattaa aagtggggaa ccaggttcgc
    28021 cttctggata ttactcagcg cgaacacttc atctttacct tcgacggtaa ctgtgatcat
    28081 ttcaccgtct accgctttga taacgccctg ccatttacga cggttttgta ccgccatacg
    28141 gagaaccaga gtcacctctt ctccgacaaa acgggcgtag tgttcagccg tgaacagtgg
    28201 gcgatcgaga cccggtgagg agacttccag gttataagca acggtgatgg ggtcttcaac
    28261 atccagcaca gcacttacct ggtggctcac atcagcacaa tcatcaacat tgatgccatc
    28321 ttcactatca atatagatgc gcagtgtgga tgtgcgaccg cgaataaatt cgatgccaac
    28381 cagttcaaaa cccagggcct caactggcgc agtaatcatc tctgttaatt tttgctctaa
    28441 tgtggacaag cccaccccca agacataaaa aaagggccta aagcccagtt attctgtagt
    28501 cagataacaa aaaaccccga taaatcgggg ctttatataa ctgaacccta taaccgcaac
    28561 tgcggtctgg agcactttcc agaaggattt tttcaaatcc cactacgaag gccgaagtct
    28621 tcacagtata tttgaaaaag gactctaagg gaaagtggtt gcgggggccg gatttgaacc
    28681 gacgaccttc gggttatgag cccgacgagc taccaggctg ctccaccccg cgcctgaaac
    28741 gtggcaaatt ctactcgttt tgggtaaaaa atgcaaatac tgctgggatt tggtgtaccg
    28801 agacgggacg taaaatctgc gggcattata gtgatccacg ccacattttg tcaacgttta
    28861 ttgctaatca cgtgaatgaa tatccagttc actttcattt gttgaatact tttaccttct
    28921 cctgctttcc cttaagcgca ttattttaca aaaaacacac taaactcttc ctgtctccga
    28981 taaaagatga ttaaatgaaa actcatttat tttgcataaa aattcagtga gagcagaaat
    29041 ccaggctcat catcagttaa ttaagcaggg tgttatttta tgacgacgat tctcaagcat
    29101 ctcccggtag gtcaacgtat tggtatcgct ttttccggcg gtctggacac cagtgccgca
    29161 ctgctgtgga tgcgacaaaa gggagctgtt ccttatgcat atactgcaaa cctgggccag
    29221 ccagacgaag aggattatga tgcgatccct cgtcgtgcca tggaatacgg cgcggagaac
    29281 gcacgtctga tcgactgccg caaacaactg gtggccgaag gtattgccgc tattcagtgt
    29341 ggcgcatttc ataacaccac tggtggactg acctatttca acacgacgcc gctgggccgc
    29401 gccgtgaccg gcaccatgct ggttgctgct atgaaagaag atggcgtgaa tatctggggt
    29461 gacggcagca cctataaagg aaacgatatc gaacgtttct accgttacgg tctgctgacc
    29521 aatgctgaac tgcagattta caaaccgtgg cttgatactg actttattga tgaactgggc
    29581 ggccgtcatg agatgtctga atttatgatt gcctgcggtt tcgactacaa aatgtctgtc
    29641 gaaaaagctt actccacgga ctccaacatg cttggtgcaa cgcatgaagc gaaggatctg
    29701 gaatacctca actccagcgt caaaatcgtc aacccaatta tgggcgtgaa gttttgggat
    29761 gagagcgtga aaatcccggc agaagaagtc acagtacgct ttgagcaagg tcatccggtg
    29821 gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga tgctggaagc taaccgcatc
    29881 ggcggtcgtc acggcctggg catgagcgac cagattgaaa accgtatcat cgaagcaaaa
    29941 agccgcggca tttatgaagc ccctgggatg gcactgctgc acattgcgta tgaacgtttg
    30001 ttgaccggta ttcacaacga agacaccatt gagcagtatc acgcgcatgg tcgtcagttg
    30061 ggccgtctgc tgtaccaggg acgttggttt gactcccagg cgctgatgct gcgggactct
    30121 ctgcaacgct gggttgccag ccagatcact ggtgaagtta ctctggagct gcgccgcggg
    30181 aacgattatt caatcctgaa taccgtctca gagaacctga cctacaagcc agagcgtctg
    30241 acgatggaaa aaggcgactc ggtgttctcg ccagatgatc gtatcggtca actgaccatg
    30301 cgtaacctgg atatcaccga tacccgcgag aaacttttcg gttatgccaa aactggcctg
    30361 ctctcctcct ctgccgcttc aggggtgccg caggtggaga atctggaaaa taaaggccag
    30421 taattcgaca tcaaccctgc ccttcagggg tggggttacc ttgattatcc tctgtaaact
    30481 cctttctgag catcacataa tcgaaaacat catggtttgc atccacaacc cgcgagtctc
    30541 ctttatagtg tgcaatcact tcctcaagcg tttgcggctg ttccggcttt gttaccccca
    30601 tccacgcatt aataatgtaa ttattgtaag ctgtcgaaaa gatgttgttt tccgttgtgc
    30661 gatccacgcc atccccaaga acggggctat accagataaa catagggaca tgatatgcct
    30721 gctggctagc ctccctgcct ccatgaaaat agacgttctt cttcgtaggg tcgcgttcca
    30781 ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc ttttaataat tcaaaaacct
    30841 gacctagcaa actatctgtg taatgaatgg agttgtcata gcaggcatcc tgatcgtcct
    30901 gcggttgaaa cacagcgctg gattgcggat aggcgctaca agccggttca tggcttccat
    30961 ttaaatgaag aacaatcagt ttcttttgct gcgtcttttg ctgtaacgct tgcgataaat
    31021 gcggcaacaa caattcatca aatcctctga catagactgt ttccatggcg cgcatggcga
    31081 tactggtaac tgctgtacca ttctgccgaa aagcggattg cgagcttagc cagaaagtct
    31141 gaaatcctgc ttgattagcc atattaataa tgttgtccgg ataattatga atgtcatgac
    31201 tcaaaacaga atcagcagtt aaagaaaggg gaaccgacag cgcagtgtaa ggtgcaccgc
    31261 ttattgcttg attaaacagt ttgatctgct ttctttgtgc ttcgacttgc ggtgtcgtag
    31321 agtgtgtata tccatacaaa gacatattgt cgacacgtac agactccccc acaatcaaca
    31381 cgtaggtatc aatacctgta tccctgactg ataattgaaa atacggcacc gtgtttgcaa
    31441 ttgagagtaa tctttgatgc tctttcgctg ctaaagcaaa ataattgaga ttgaaaaacg
    31501 gcgtataggt agcaaatcgc gacgctagta tatatggact gaacgcctct ttattttttg
    31561 catctttata agcaaattga caag
//
LOCUS       DACADF010000044        30953 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.044, whole genome shotgun sequence.
ACCESSION   DACADF010000044 DACADF010000000
VERSION     DACADF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.044"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            164..2836
                     /gene="rcsD"
                     /locus_tag="ISB30_001912"
     CDS             164..2836
                     /gene="rcsD"
                     /locus_tag="ISB30_001912"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAP1989547.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            2853..3503
                     /gene="rcsB"
                     /locus_tag="ISB30_001913"
     CDS             2853..3503
                     /gene="rcsB"
                     /locus_tag="ISB30_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAP1989548.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3703..6552)
                     /gene="rcsC"
                     /locus_tag="ISB30_001914"
     CDS             complement(3703..6552)
                     /gene="rcsC"
                     /locus_tag="ISB30_001914"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAP1989549.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVNKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            6719..8545
                     /gene="atoS"
                     /locus_tag="ISB30_001915"
     CDS             6719..8545
                     /gene="atoS"
                     /locus_tag="ISB30_001915"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HAP1989550.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            8542..9927
                     /gene="atoC"
                     /locus_tag="ISB30_001916"
     CDS             8542..9927
                     /gene="atoC"
                     /locus_tag="ISB30_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HAP1989551.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRVIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            10123..10785
                     /gene="atoD"
                     /locus_tag="ISB30_001917"
     CDS             10123..10785
                     /gene="atoD"
                     /locus_tag="ISB30_001917"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HAP1989552.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            10785..11435
                     /locus_tag="ISB30_001918"
     CDS             10785..11435
                     /locus_tag="ISB30_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HAP1989553.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            11432..12754
                     /locus_tag="ISB30_001919"
     CDS             11432..12754
                     /locus_tag="ISB30_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HAP1989554.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            12785..13969
                     /locus_tag="ISB30_001920"
     CDS             12785..13969
                     /locus_tag="ISB30_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAP1989555.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLNPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(14043..14819)
                     /locus_tag="ISB30_001921"
     CDS             complement(14043..14819)
                     /locus_tag="ISB30_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAP1989556.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYFGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(14824..16473)
                     /locus_tag="ISB30_001922"
     CDS             complement(14824..16473)
                     /locus_tag="ISB30_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAP1989557.1"
                     /translation="MNWYRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSVLPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     SALVRSCGLYFAPQRLHIGADMWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATALNRVLPVPTQVASGQCVLVDLFARYPLKKVTEEKST
                     TAVKLGVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNADREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRHATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(16474..20868)
                     /locus_tag="ISB30_001923"
     CDS             complement(16474..20868)
                     /locus_tag="ISB30_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAP1989558.1"
                     /translation="MRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRI
                     VVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIVKP
                     SRYVQNSQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPL
                     GKQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEIL
                     WTDGLGVMTRGVTDGSGTLQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLY
                     IFTDRPLYRAGDRVDVKVMGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDA
                     RNGGQGSFRLPENAVAGGYELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTG
                     EAVSGKLQLLYPDGEPVKDARVQLSLRAQQLSMVGNDLRYAGRFPVSMEGSETVSDDN
                     GHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGE
                     SVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELQSGGKSFTVNFAKPGNYNLTLRDK
                     DGLILAGLSHAVSGKDSTSHSGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLER
                     DRVEQQSLLSHPANWLTLQRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAG
                     IKVAVPQLDIRVKTDKTHYQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEI
                     APNIGKFFYPLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREE
                     VDTAAWMPSLTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLY
                     MKWSMPTVYRMGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQ
                     NIQQSGLLSAELQQNGQVQDSISTKLSFVDNRWPVEQQKNVMLGGGDNALMLPEQASN
                     IRLQSSETPQEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQ
                     MIQDNRLRLMQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQH
                     MLDSYAEQADNMPLLHRALVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTS
                     DINDSMILDTPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAH
                     TVVLLNSGGDATQAAAILSGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAK
                     HKLTGGGEYWRWVGQGVPDILSFGDELPPQNVQVRWREPAKTAQQSNIPVTVERQLYR
                     LIPGEEEMSFTLQPVTSNEIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERT
                     TWGISVNKPNAGKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVL
                     PPARYVRSYAPAQQSVAPGSEWIGMQVK"
     gene            complement(21012..21635)
                     /locus_tag="ISB30_001924"
     CDS             complement(21012..21635)
                     /locus_tag="ISB30_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAP1989559.1"
                     /translation="MRHGLLALICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(21632..23320)
                     /locus_tag="ISB30_001925"
     CDS             complement(21632..23320)
                     /locus_tag="ISB30_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAP1989560.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYSGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWETRFGLEARTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFLALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(23469..26096)
                     /gene="gyrA"
                     /locus_tag="ISB30_001926"
     CDS             complement(23469..26096)
                     /gene="gyrA"
                     /locus_tag="ISB30_001926"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAP1989561.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            26243..26965
                     /locus_tag="ISB30_001927"
     CDS             26243..26965
                     /locus_tag="ISB30_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAP1989562.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(27105..30863)
                     /gene="yfaL"
                     /locus_tag="ISB30_001928"
     CDS             complement(27105..30863)
                     /gene="yfaL"
                     /locus_tag="ISB30_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HAP1989563.1"
                     /translation="MRIISLRKEYLSLLPSMIASLFSANGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNIQDWSIADGQWLIFSGMVNNASGGAVFLQHSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDKT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEMSIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQALDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAVSLQNGIAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTTVV
                     INPITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVNPTPPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDHAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNRGYAVGLTSSWYQHGNQKQGAWLDSWLQ
                     YAWFNNDVSEQDDGVDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQMRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     AVKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
BASE COUNT         7459 a         8329 c         7595 g         7570 t
ORIGIN      
        1 ttgtacagca aaatggcgct tgtacatcta tttcccccaa tgccggatga taaatatcac
       61 tggagaatag agaatcatca atcaggtaag agtctggaat ttcacactgt accctttata
      121 ctgccctatc acttcgcgaa gttttaacag gtcataaaca cgaatgcgtc agaaagagac
      181 aacggccacg acccgctttt cactcctacc ggggagcatt acccgcttct ttttactgtt
      241 gatcattgtg ttactggtga cgatgggtgt aatggtacaa agcgccgtta acgcctggct
      301 gaaagataaa agttaccaga ttgtcgacat tacccacgca atccaaaagc gcgtcgatac
      361 ctggcgttac gtgacctggc agatctacga caacattgcc gcgacgacct ccccctcctc
      421 cggcgaaggt ttacaagaga cgcgcctgaa acaggatgtc tactatctgg agaaaccacg
      481 ccgcaaaacg gaagcgttaa tctttggctc tcacgacaac tcaacgcttg agatgactca
      541 acggatgtcc acctatctgg acacattgtg gggcgcagaa aatgtaccgt ggtcgatgta
      601 ttacctgaat ggtcaggata acagtctggt gctgatctca accctgcccc tcaaagatct
      661 cacctccgga tttaaagaat cgaccgtcag cgacattgtt gattcacgtc gtgcagagat
      721 gttgcaacag gccaacgccc tcgatgaacg cgaaagtttt tctaacatgc gccgcctggc
      781 ctggcagaac ggtcattact ttaccttgcg taccacgttc aaccagccgg gacatctggc
      841 aacggtcgtg gcttttgatc tgccgattaa tgatttgatc ccaccgggta tgccgctgga
      901 cagtttccgc cttgagccag acgcgacggc aacgggaaac aatgaaaatg agaaagaagg
      961 gacggatagc gtcagtatcc actttaacag tacgaagatt gaaatctcct cggcactcaa
     1021 ctctaccgat atgcgcctgg tctggcaggt tccttacggc accttactgc tggatacgtt
     1081 gcaaaacatt ctgctgccac tgctgctgaa catcggtttg ctggcgctgg cgttatttgg
     1141 ctataccaca ttccgccatt tctccagccg cagtacagaa agcgtcccca gcacggcggt
     1201 caataacgaa ttgcgcattt tacgggcaat taatgaagag atagtctcac tgctgccgct
     1261 cggcctgctg gttcacgatc aggaatcaaa ccgcacagtc ataagtaaca aaattgccga
     1321 tcatttgctg ccgcatttga atctgcaaaa catcaccacc atggcggaac agcatcaggg
     1381 gattattcag gcgacgatca ataacgagct gtatgagatc cgcatgttcc gcagccaggt
     1441 tgcgccgcgc acacaaattt tcattattcg cgatcaggat cgcgaagtgc tggtaaacaa
     1501 gaaactcaag caggcgcagc gtctgtatga gaaaaaccag caggggcgga tgacctttat
     1561 gaaaaacatt ggcgatgcgc tgaaagaacc cgcacagtcc ctggcggaga gcgcggctaa
     1621 actcaacgcc ccggaaggca aacaactggc gaatcaggct gatgtgctgg tgcgactggt
     1681 cgatgaaata cagttagcga acatgcttgc ggacgatagc tggaaaagtg agacggagct
     1741 gttctccgtg caggatttaa ttgatgaagt agtgccttca gtgttgcctg ccatcaagcg
     1801 taaaggtctg caactgctga ttaacaatca tctgaaagca cacgatatgc gccgcggcga
     1861 tcgcgatgcc ttacgacgta ttttgctgct actgatgcaa tatgccgtga cctcaacgca
     1921 attaggaaaa atcacccttg aggttgatca ggatgagtcc tccgaagacc gcctgacgtt
     1981 ccgcattctg gacactggag aaggcgtaag cattcatgaa atggataatt tgcacttccc
     2041 gtttatcaac cagacccaaa acgatcgcta tggcaaggcg gacccgctgg cattctggct
     2101 gagcgatcaa ctggcgcgta aactgggcgg tcatttaaac atcaaaacgc gggatgggct
     2161 tggtacacgc tactctgtgc atatcaaaat gctcgcagct gacccggaag ttgaagagga
     2221 agaagagcga ttactggatg atgtctgcgt aatggtggat gttacttcgg cagaaattcg
     2281 gaatattgtc actcgccagt tagaaaattg gggtgcaacc tgtatcacac ccgatgaaag
     2341 attaattagt caagattatg atatcttttt aacggataat ccgtctaatc ttactgcctc
     2401 tggcttgctt ttaagcgatg atgagtctgg cgtacgggaa attgggcctg gtcaattgtg
     2461 cgtcaatttc aatatgagca acgctatgca ggaagcggtc ttacaattaa ttgaagtgca
     2521 actggcgcag gaagaggtga cagaatcgcc tctgggcgga gatgaaaatg cgcaactcca
     2581 tgccagcggc tattatgcgc tctttgtaga cacagtaccg gatgatgtta agaggctata
     2641 tactgaagca gcaaccagcg actttgctgc gttagcccaa acggctcatc gtcttaaagg
     2701 cgtatttgcc atgctaaatc tggtacccgg caagcagtta tgtgaaacgc tggaacatct
     2761 gattcgtgag aaggatgttc caggaataga aaaatacatc agcgacattg acagttatgt
     2821 caagagcttg ctgtagcaag gtagcctatt acatgaacaa tatgaacgta attattgccg
     2881 atgaccatcc gatagtcttg ttcggtattc gcaaatcact tgagcaaatt gagtgggtga
     2941 atgttgtcgg cgaatttgaa gactctacag cactgatcaa caatctgccg aaactggatg
     3001 cgcatgtgtt gattaccgat ctctccatgc ctggcgataa gtacggtgat ggcattacct
     3061 taatcaagta catcaagcgc catttcccaa gcctgtcgat cattgttctg accatgaaca
     3121 acaacccggc gattcttagt gcggtattgg atctggatat cgaagggatc gtgctgaaac
     3181 aaggtgcacc gaccgatttg ccgaaggctc tcgccgcgct gcagaaaggg aagaaattta
     3241 ccccggaaag cgtttctcgc ctgttggaaa aaatcagtgc tggtggttac ggtgacaagc
     3301 gtctctcgcc aaaagagagt gaagttctgc gcctgtttgc ggaaggcttc ctggtgaccg
     3361 agatcgctaa aaagctgaac cgcagtatta aaaccatcag tagccagaag aaatctgcga
     3421 tgatgaagct gggtgtcgag aacgatattg ctctgctgaa ttatctctct tctgtgacgt
     3481 tgagtccggc agataaagac taatcacctg taggccagat aagacgcgtc agtgtcttat
     3541 ctggcatttg cacggattgc cggatgcggc gtaaacgcct tatccgtcct acgattccca
     3601 ttatttcaac agattacatt aaagtaggcc agataagacg cgtcagcgtc gcatctggca
     3661 tttgcactga atgccagatg cggcataaac accttatccg tcctacgatt cccgcgattt
     3721 cctgaccctc tcggcatata ccgtcagcgt ctgttttatc acatccagcg ttaccggctt
     3781 cgacaggcag ctgtccatac cggactccag acaccgctgc ttctcttcag ccaacgcatt
     3841 agcagttact ccgattaccg gcaacgtcag tcccaactga cgaatgcgtt gcgtcaagcg
     3901 gtaaccatcc atatttggca tattgacgtc gctaagcacg atatcaatat gattcttgtt
     3961 aagtacatta agcgcatcga cgccatcatt cgcggtttta cattgatagc ccaacgatcc
     4021 caactgatct gccagcagac gccggttaat cggatgatca tccacgacca gaatcatcat
     4081 atcgtcatta tcgctgaccg ctttgtccgt cgacggcaga gcgttagcag gatcgtcgct
     4141 ctccatctcg atcaaataaa tacgcgccaa caatgccggt agctcatgcg gagcagccac
     4201 actatgtacc cactcccctg gcgctttctc cagcggaata ccaatatggc gacgacagaa
     4261 ggtcactacc gctctgccct gccatttttt gttcactacc tcgtcagtaa ttaacacatc
     4321 ttcgggagtc ggttcctgcc cttcgtatgt tgtaacgacg atgccgctgc gctgcaaact
     4381 ggtttccagg aactgacaga gcgacgcatt gcggaccgcc agccagcagc gtttaccact
     4441 caacccttcc acaccttttt tctgcgggta ctgagcgccg tataacggaa tacgcacggt
     4501 aaactggctg cccattcccg gttctgaatc taccgagatg tcgccgtcca tcatgctgat
     4561 cagtttttca caaatcgcca gacccagacc agtcccctgg aagttacgct gtacgcccgt
     4621 tccgacctgg aagaagggat caaacaagcg caccacttct ttcgccggta tccccacgcc
     4681 ggtatcgcga acacggatag agagataatc gccatcagcg cgaacatgca aaactataca
     4741 gccggtatcg gtgaatttta tggcgttact caacaggttg gagatgacct gctgtaaacg
     4801 catcgggtcg ccatttaagg ccactggcac atccggttca ataaagcagt acaagcctaa
     4861 ctgcttgcgt accaccagcg gtaaatagtt ggcggtgatg tggttcatca cttcacgcgg
     4921 tgaaaactca cgcggttcga tcttcaactg ttctgattca attttcgaga aatcgagaat
     4981 atcgctgata attttcaaca acaggctgga agagttgttc attgccgtca ccagacgatc
     5041 gacgcctttc ggtaactctt tggtttgcaa cagatccagg ttaccgataa tgccatacag
     5101 cggcgttcgc agctcatgac tgacggtggc caggaacatc gattttgact ggctcgcctg
     5161 ttccgctgct tgtgccatct cctgcaacga ctcttccatc ttcacgcgcg aagaaacatc
     5221 caccagcaca caaatggcca cgttttcatt acgatagcgc gaatggacga agctgatttg
     5281 cagattggta ttgttgctgg tcaggacatc aacaaaattg acctgctgcc cacagataat
     5341 ttgtgtcagc cgttggcggt cctcatgcgt aagcatattg agataagtat gcgccagttc
     5401 gttacttaaa atattgacgc catcagcggt acgcaaaatg cagataccca ctggcgcgga
     5461 ggcgacaatc ttgcgattaa actgctcatg ttcttccagt cgcagggcgt cgctttccgc
     5521 cggaatgaaa atacgccgct cgtacatccg cgcgagagta aacaatgcag ctccggcaag
     5581 cacattcagc aaaattgcgt taaggatcaa catgcgaatg cgttccagca ccttatcaac
     5641 tggcactgaa taaacgatgc ttagcgatga gggtggcaga tttttcttca gcaccagttc
     5701 ccggaaccct tccgtatagc caaaccagga gcgttcctgc atccagcgag gatcgccctt
     5761 aattttgctt tctggtccgg taagcgaaat cagggtatga ccattttcat caagaatggt
     5821 aacccccatc ggcaacgtac caggtaagaa aaagttctcc atccggatgg tttgctcgac
     5881 acccaaaagc gcctgcaacc ggttcgccag ataaactggc gtcaaggcgt aaaaataccc
     5941 gacgccaggg cgcggacctt cgctgatcca gtagaggtta ctgccactat catcttgtgg
     6001 tgcatttcga tatttattaa tgcgttcatg caaagctttt agcgcggtat cgcgttccac
     6061 tggcatatca cgcagaccga aattggccat gcagaggtta tcgctgccga ttaaaaatac
     6121 ccggtttaga tcgtaagccg cagaaaaatt atcgcgccag tagcgcataa accacgccaa
     6181 tgactccaga gaacctcgcc aggtgttact cattgcggaa caatcagagt cagcaaacag
     6241 cggttcaaac gcaggcacat ccgtctgcgt ttctcgtcca cgcggggaaa gcacaccgtt
     6301 ttctgccgat aagcgatttt cagctatgta cttcagctct ttcatcacat cagaggtgcg
     6361 ttgaataaag cgctgagcct gatcggaact cagattaaat tcctgacgaa tttccgattc
     6421 tcgctgatgt aacgcattaa cgatgtaaaa aacggatgaa aaagcaatca acagccagag
     6481 cactaacgcc aatgctctga acatgtagcg cgaggctttc agggttgtac gaaaagaagc
     6541 aaggtatttc aaaggggcga agctccgcct caggtgaccg atggagtgtg gttaaggtag
     6601 cggtaaaagc gtgttaccgc aatgttctct cttctctgga atatgataca ccgccgagaa
     6661 atcatcacct taacctctga taatcgtcat ataccggaca agactagtgg atttcagcat
     6721 gcattatatg aagtggattt atccacgccg cttacgcaat caaatgatcc tgatggcaat
     6781 cctgatggtc attgtcccaa cgcttactat tggttatatc gtagaaacgg aaggacgttc
     6841 agcagtctta tctgaaaaag agaaaaaact ttctgccgtg gtcaacctgc ttaatcaggc
     6901 gctaggcaat cgctacgatc tctacatcga cttaccgcgt gaggagcgta tccgcgcatt
     6961 aaatgcagaa cttgccccca ttaccgaaaa tatcactcac gccttccctg gcatcggcgc
     7021 tggttattac aacaaaacgc tggatgcgat aatcacctac gcgccttcag cgctatatca
     7081 gaataatgtg ggcgttacca ttgccgcaga tcaccctggt cgcgaagtca tgcgtacaaa
     7141 tacccctttg gtttattcag gcaggcaggt gcgcggcgat attttgaatt caatgattcc
     7201 cattgagcgt aatggtgaaa tcctcggcta tatctgggcc aatgaattaa ccgaagatat
     7261 tcgccgccag gcctggaaaa tggatgtgag gattatcatt gtgctcaccg ccggtttgct
     7321 gataagcctg ctgttgattg tccttttctc ccgtcgcctg agcgccaata tagatatcat
     7381 caccgatggc ctctcgactc tggctcaaaa tattcccact cgattaccac aattgcccgg
     7441 tgaaatgggg caaatcagtc agagtgttaa caacctcgcc caggcactgc gtgaaacgcg
     7501 aacacttaac gatctgatta ttgaaaacgc tgccgatggc gtcattgcca ttgaccgcca
     7561 gggtgatgta accaccatga acccagcagc agaagtcatc actggctatc aacgtcatga
     7621 actggtaggg cagccttact ccatgttgtt cgacaatact cagttctaca gtcccgtact
     7681 ggatacgctg gaacatggca ccgaacatgt tgcactggag atcagttttc caggccgtga
     7741 ccgcaccatt gaactcagtg tcacgaccag tcgtattcat aacacgcacg gtgaaatgat
     7801 aggtgctttg gtgattttct ctgatttaac tgcccgcaaa gaaacccagc gccgcatggc
     7861 gcaagcagaa cgcctcgcca cactgggtga gctgatggct ggcgtggcgc atgaagtacg
     7921 taatccgcta acggctattc gtggttatgt acagatcttg cgccaacaaa cccgtgaccc
     7981 aatacatcag gaatatctgt ccgtagtact caaagaaatc gattcaatta acaaagttat
     8041 tcagcaattg ctcgaatttt cacgcccacg ccacagtcaa tggcaacaag tcagcctcaa
     8101 tgcattggtt gaagaaactc tggtactggt acaaaccgcc ggcgtacaag cgcgggtcga
     8161 cttcataagc gaactggata atgaattaag cccgattaac gccgatcgtg aactgctcaa
     8221 acaggtacta ctgaatatcc tgatcaatgc cgtccaggct atcagtgcac gagggaaaat
     8281 tcgcattcga acctggcagt atagcgactc gcaacaggcc atttcgatag aggacaacgg
     8341 cagtggcatt gatctctcgc tgcaaaaaaa gatcttcgat ccctttttca ctaccaaagc
     8401 ctcaggaacc gggcttggtc tggcgttaag tcaacgcatc attaatgccc atcagggtga
     8461 tattcgcgtc gccagtttgc cgggctacgg cgcaaccttc acgcttattt taccgatcaa
     8521 cccgcaggga aatcagactg tatgactgct attaatcgca tccttattgt ggatgatgaa
     8581 gataatgttc gccgtatgct gagcaccgct tttgcactac aaggattcga aacacattgt
     8641 gcgaacaacg ggcgcacagc attacacctg tttgccgata ttcaccctga cgtggtgttg
     8701 atggatatcc gcatgccaga gatggacggc atcaaggcac taaaggagat gcgcagccat
     8761 gagacccgga cacccgttat tctgatgacg gcctatgctg aagtggaaac cgccgtcgaa
     8821 gcactacgct gcggagcctt cgactatgtt atcaaaccgt ttgatctcga tgagttgaat
     8881 ttaatcgttc agcgcgcttt acaactccag tcaatgaaaa aagagatccg tcatctgcac
     8941 caggcactga gcaccagctg gcaatggggg cacattctca ccaacagccc ggcgatgatg
     9001 gacatctgca aagacaccgc caaaattgcc ctttctcagg ccagcgtctt gattagcggt
     9061 gaaagcggca ccgggaaaga gttgattgcc agagcgattc actacaattc gcggcgggca
     9121 aaggggccgt tcattaaagt caactgcgcg gcactgccgg aatcgttgct cgaaagtgaa
     9181 ctgtttggtc atgaaaaagg tgcatttact ggtgcacaaa ccttgcgtca gggattattt
     9241 gaacgtgcca acgaaggtac tctgctcctc gacgaaattg gcgaaatgcc gctggtactg
     9301 caagccaaat tactacgcat tctgcaggaa cgggaatttg aacggattgg cggccatcag
     9361 accataaaag ttgatatccg cgtcattgct gccaccaacc gcgacttgca ggcaatggtg
     9421 aaagaaggca ccttccgtga agatctcttt tatcgcctta acgttattca tttaatactg
     9481 ccgcctctgc gcgatcgccg ggaagatatt tccctgttag ctaatcactt tttgcaaaaa
     9541 ttcagtagtg agaatcagcg cgatattatc gacatcgatc cgatggcaat gtcactgctt
     9601 accgcctggt catggccggg aaatattcga gagctttcca acgttattga acgcgccgtc
     9661 gtgatgaatt caggcccgat cattttttct gaggatcttc cgccgcagat tcgtcagcca
     9721 gtctgtaatg ctggtgaagc aaaaacagcc cctgtcggtg agcgtaattt aaaagaggaa
     9781 attaaacgcg tcgaaaaacg catcattatg gaagtgctgg aacaacaaga aggaaaccga
     9841 acccgcactg ctttaatgct gggcatcagt cgccgtgcat tgatgtataa actccaggaa
     9901 tacggtatcg atccggcgga tgtataatgc caaaacttgc tatgcagaaa tttgcacagt
     9961 gcgcaatttt ctgcatagcc gctcattctc cttatgaacc cccatctaat ttatcctttt
    10021 ctattcaatt agttaaataa caacattcaa taatctcatt ctggcactcc ccttgctatt
    10081 ccctgactgt acccacaacg gtgtatgcaa gagggataaa aaatgaaaac aaaattgatg
    10141 acattacaag acgccaccgg cttctttcgt gacggcatga ccatcatggt gggcggattt
    10201 atggggattg gcactccatc ccgcctggtt gaagcattac tggaatctgg tgttcgcgac
    10261 ctgacattga tagccaatga taccgcgttt gttgataccg gcatcggtcc gctcatcgtc
    10321 aatggtcgag tccgcaaagt gattgcttca catatcggca ccaacccgga aacaggtcgg
    10381 cgcatgatat ctggtgagat ggacgtcgtt ctggtgccgc aaggtacgct aatcgagcaa
    10441 attcgctgtg gcggagctgg acttggtggt tttctcaccc caactggtgt cggcaccgtc
    10501 gtagaggaag gcaaacagac actgacactc gacggtaaaa cctggctgct cgaacgcccg
    10561 ctgcgcgccg acctggcgct aattcgcgct catcgttgcg acacacttgg caacctgacc
    10621 tatcacctta gcgcccgcaa ctttaacccc ctgatagccc ttgcggctga tatcacgctg
    10681 gtagagccgg atgaactggt cgaaaccggc gagctgcaac ctgaccatat tgtcacccct
    10741 ggtgccgtta tcgaccacat catcgtttca caggagagca aataatggat gcgaaacaac
    10801 gtattgcgcg ccgtgtggcg caagagcttc gtgatggtga catcgttaac ttagggatcg
    10861 gtttacccac aatggtcgcc aattatttac cggagggtat tcatatcact ctgcaatcgg
    10921 aaaacggctt cctcggttta ggcccggtca caacagcgca tccagatctg gtgaatgctg
    10981 gcggacaacc gtgcggcatt ttacccggtg cagccatgtt tgatagcgcc atgtcatttg
    11041 cgctaatccg tggcggtcat attgatgcct gcgtgctcgg cggtttgcaa gtagacgaag
    11101 aagcaaacct cgcgaactgg gtagtgcctg ggaaaatggt gcccggtatg ggtggcgcga
    11161 tggatctggt gaccgggtca cgcaaagtga tcatcgccat ggaacattgc gccaaagatg
    11221 gttcagcaaa aattttgcgc cgctgcacca tgccactcac tgcgcaacat gcggtgcata
    11281 tgctggttac tgaactggct gtctttcgtt ttattgacgg caaaatgtgg ctcaccgaaa
    11341 ttgccgacgg gtgtgattta gccaccgtgc gtgccaaaac agaagctcgg tttgaagtag
    11401 ctgccgatct gaatacgcaa cggggtgatt tatgattggt cgcatatcgc gttttatgac
    11461 gcgttttgtc agccggtggc ttcccgatcc actgatcttt gccatgttgc tgacattgct
    11521 aacattcgtg atcgcgcttt ggttaacacc acaaacgccg atcagcatgg tgaaaatgtg
    11581 gggtgacggt ttctggaact tgctggcgtt tggtatgcag atggcgctta tcatcgttac
    11641 cggtcatgcc cttgccagct ccgctccggt gaaaagtttg ctgcgtactg ccgcctccgc
    11701 cgcaaagacg cccgtacagg gcgtcatgct ggttactttc ttcggttcag tcgcttgtgt
    11761 catcaactgg ggatttggtt tggttgtcgg tgcaatgttc gcccgtgaag tcgcccggcg
    11821 agtacccggt tctgattatc cgttgctcat tgcctgcgcc tacattggtt ttctcacctg
    11881 gggtggcggc ttctctggct caatgcctct gttggctgca acaccgggca acccggttga
    11941 gcatatcgct gggctgatcc cggtgggcga tactctgttc agtggtttta atattttcat
    12001 cactgtggcg ttgattgtgg tgatgccatt tatcacccgc atgatgatgc caaaaccgtc
    12061 tgacgtggtg agtatcgatc cgaaactact catggaagag gctgattttc aaaagcaact
    12121 accgaaagat gctccaccgt ccgagcgact ggaagaaagc cgcattctga cgttgatcat
    12181 cggcgcactc ggtatcgctt acctggcgat gtacttcagc gaacatggct tcaacatcac
    12241 catcaatacc gtcaacctga tgtttatgat tgcgggtctg ctgctacata aaacgccaat
    12301 ggcttatatg cgtgctatca gcgcggcagc acgcagtacc gccggtattc tggtgcaatt
    12361 ccccttctac gcggggatcc aactgatgat ggagcactcc ggtctgggcg gactcattac
    12421 cgaattcttc atcaatgttg cgaacaaaga caccttcccg gtcatgacct tttttagttc
    12481 tgcactgatt aacttcgccg ttccgtctgg cggcgggcac tgggttattc aggggccgtt
    12541 cgtgatgccc gcagcccagg cgctgggcgc tgatctcggt aaatcggtaa tggcgatcgc
    12601 ctacggcgag caatggatga acatggcaca accgttctgg gcgctgccag cactggcaat
    12661 cgccggactc ggtgtccgcg acatcatggg ctattgcatc actgccctgc tcttctccgg
    12721 cgtcattttc gtcattggtt taacgctgtt ctgacggcaa ccctacagac agaaggaata
    12781 taaaatgaaa aattgtgtca tcgtcagtgc ggtacgtact gctatcggta gttttaacgg
    12841 ttcactcgct tccaccagcg ccatcgacct gggggcgaca gtaattaaag ccgccattga
    12901 acgtgcaaaa atcgattcac tacacgttga tgaagtgatt atgggtaacg tgttgcaagc
    12961 cggactgggg caaaatccgg cacgtcaggc gctgctaaaa agcggactag ctgaaacggt
    13021 gtgcggattc acggtcaaca aagtgtgcgg ttcaggtctg aaaagcgtgg cgcttgctgc
    13081 acaggcgatt caggcaggtc aggcacagag cattgtggcg gggggtatgg aaaatatgag
    13141 tttagcgccc tacttactcg atgcaaaagc acgctctggt tatcgtcttg gagacggaca
    13201 ggtttatgac gtaatcctgc gcgatggcct gatgtgcgcc acccatggtt atcatatggg
    13261 gattaccgcc gaaaacgtgg ctaaagagta cggaattacc cgtgaaatgc aggatgaact
    13321 ggcgctacat tcacagcgta aagcggcagc cgcaattgag tccggtgctt ttacagccga
    13381 aatcgtcccg gtaaatgttg tcacccggaa gaaaaccttc gtcttcagtc aagacgaatt
    13441 cccgaaagcg gattctacgg ctgaagcgtt aggcgcattg cgcccggcct tcgataaagc
    13501 aggaacagtc accgccggga acgcgtcagg tattaacgac ggtgctgccg ctctggtgat
    13561 tatggaagaa tctgcggcgc tggcagcagg cctgaatccc ctggcacgca ttaaaagtta
    13621 tgccagcggt ggcgtgcccc ccgcattgat gggtatgggg ccagtacctg ctacgcaaaa
    13681 agcgttacaa ctggcggggc tgcaactggc ggatattgat ctcattgagg ctaatgaagc
    13741 atttgctgca cagttccttg ccgttgggaa aaccctgggc tttgatcctg agaaagtgaa
    13801 tgtcaacggc ggggccatcg cgctcggaca tcctatcggt gccagtggtg ctcgtattct
    13861 ggtcacacta ttacatgcaa tgcaggcacg cgataaaacg ctggggctgg caacactatg
    13921 cattggtggc ggccagggaa ttgcgatggt gattgagcgg ttgaattaac caataaaaac
    13981 gcccgatagc gaaagttatc gggcgttttc ttgaacatcg acggcgaagg ttaccccatt
    14041 aatcaccagt caaaactttt caccagcgtc agctcgccag cattacgcat cggtacaata
    14101 aaagtttcct gtttctcatt gaccgaccct tcatcggtga tcagcgtcag ttgggccgta
    14161 gttaattccg tttcactgcg cccgccgaag tagttgatat acacctgata acggccgtga
    14221 actggcgctg gcatggcgaa aatctcgggt ccgtaccctg tcgtgacatc catatccagt
    14281 gcgccactgt ttttcagcac tgtgttaccg taccaggcat gttcaccgtc aggcgtaacg
    14341 acatgaagat cgaggtcggt attgtccgta tcccaggaga gaaccagccg taaacgcgcg
    14401 cgaatcgccc cagcacccgg cgttgagtaa aactgcattt tttgtcggct ttgcccatct
    14461 gggctgatga cctgcacgct gttgctgcct tcagtgaaaa tataagggcg tgcaaaagaa
    14521 ccatctgatt caattctctg cggcatactg gcaccgttga ccaccagccg gccctgctga
    14581 actttacccg ccgcagcata attctttatt ttgccgcgga tctgcgctga gatattttga
    14641 tcatctgcca tgttgacgga cgacgccggg taattgatcg tttgtgaaaa actggcatct
    14701 tcgccctctg ccggatgcca gccagagagc ggtgcatcaa tctcgacttc ctgcacacct
    14761 tcactgtgag cgacaggcga aagcgccacc agtaacaacg gaagaaaaat ctttcgcata
    14821 gcattagtcc attaataatt gtcgggtcag cgtttcgata tagttctcat caagcccggt
    14881 gggataacca tcaaaggcca ggtgcagata ctcatgggtt aaatccagcc gatcctgaag
    14941 cgtaaagaaa ttacgaataa acagccgttt ctgctgccga tcggtatagg ggaaacctga
    15001 gactaaacga cagaccgcaa acacgtctgg ttcgttgtat cccgtctcac cttgtaatat
    15061 acgccgccac tgcggcattt ttttcgccag ccaggctttc gctttgggta ataactggca
    15121 ggttgagcgt ggcgctcccc agcggctaag gctgttgtcg ggataagcaa acgcgagtat
    15181 ctgatcataa cgctctccct gccccgcctg ggcagtagcg tgccgccagg aaagcgtccc
    15241 ttcggtagcg cgactgccat gataatgaac cggatcgccc gcatagatga gatcctgcgt
    15301 ccaggcggtc atcgttcgcg cccccgtggt agctggcgaa gcagaaacac gctgcgtagc
    15361 gctgctgtcc gggatggtca ggcaatcgcc ttcccgatcg gcgttttgct gtaaaaacgt
    15421 gcgaatcgcc acggtcatgg ctttcgctgc ctcaggaggc gtacttttcg cctcgcgatc
    15481 cagaactctg gcaacgtact cttcacgatc gagatgcgac tgtaatttca gtttgccctt
    15541 taccgtcagt aacgttgtct caccgtggct gacaaatgtc atgtggttac cattggcaaa
    15601 cgtcacacga tagcgcccat tgagcacgcc tagtttgact gccgtcgtac tcttttcctc
    15661 agtaactttc ttcagcggat aacgagcaaa cagatccacc agcacacatt gcccactggc
    15721 aacttgcgtc ggcactggca aaacccgatt aagcgccgtc gcataacggg tcagtaccgt
    15781 tttgctggtg ccactgccag taacccacag cggtgtacca tcggttaacc agccagcaaa
    15841 accaccctga cggatttcct gtttatcatc ggcaaaccag ctccaggttt tcacccgtac
    15901 ccgactaccg agcatggatg cgacaccgat tttcgcctcg tccagcacca catccagtaa
    15961 gacttcctga gctttgtttt gcgcaggtaa cgtcgctaat gaatcaagca gcgatttaac
    16021 cgttaccgag gtttctggct tcagtgtagt aagagacgct aaccacgcag gtgcctgacg
    16081 ctgttgccag tattgccccc acatatctgc gccaatatgc aaccgctgcg gcgcaaaata
    16141 gagtccacag gaacgtacca gcgcggaatc gcgcgttatg ctctccccag cctgacaaca
    16201 ataaacctct tccggggagt tgccgcgaca ctggtatggc tgctccggtt gatgggtatc
    16261 ctccagccag gcgtaaacat acagtttcca caaactccct aatggcgtgg tgagcgtgtc
    16321 aggtaatacc gagactttgg tcacgcctga tgacgacagc tgatacagtt gatcgtgctg
    16381 cgcaccacgc agcgccagct gtaacggcgt ctcctccgca agcgttggca gagttaccaa
    16441 cgccaatagc cagacgattc tgtaccagtt cacttatttc acctgcatcc cgatccattc
    16501 gctccctggc gcaacacttt gctgtgcagg tgcataggaa cgcacataac gggcaggcgg
    16561 cagaacgaat tgcccttttt gcgagaaacg cagcaaatgg cggaaggtga ccgttcccgt
    16621 cagttctttt accggcacca tataggccag ttcacccatt tcattgcgcg ctttttccag
    16681 cagttgtcct tgctgttttc cggcgttcgg tttattgacc gaaatgcccc aggttgtacg
    16741 ctcaacgtca gcccccggcg gcagcggtac ttccacctga ccatagcgca gaacggcatc
    16801 ctgctcgctg gtaagcgtga tttcatcgag atacagcgca tcgctgtcaa tctcattgct
    16861 ggtgacaggc tgtaaggtaa agctcatctc ttcttcaccg gggataagcc gatacaactg
    16921 gcgttcaacg gtcaccggaa tattactttg ttgagccgtt tttgccggtt cacgccagcg
    16981 gacctgcacg ttttgcggcg gtaattcgtc accaaaagag agaatgtccg gcacgccctg
    17041 accaacccaa cgccagtatt cgcctcctcc ggttaactta tgtttggccc atgcgcccgc
    17101 aggcgcaggt aacacaaccg gaggcatcgt cgccatatat ttcgccagcc agttcatggc
    17161 caacgcgcgc tcaatggtgg attgctcagc ggttaaacca cttaaaatag cggccgcctg
    17221 ggtagcgtcg cctccactat tgagcagcac aaccgtgtgc gccagcggtt ggttgctgtt
    17281 tgctgcttta tcccacgcat attgctgaac ctgtggcatc acagtggact tcaactgcgc
    17341 ttttttcagc aacgtcatgg ttaagacgtt tgccaccgca tctgccagtg gagattcagg
    17401 tgtatcgagg atcatgctgt cattgatatc gctggtgtct tcctcagaga aatcttcagt
    17461 tttagttccg cgccgggcga tagcttcatc caaccctttc aacaacgttt tgcacggcag
    17521 attcatctcc tgtgcccacg ccagcaccag cgcccgatgc aataacggca tgttgtccgc
    17581 ctgctcggca taactgtcga gcatatgttg ccagtattcc ggttgctgcg ttacgccgag
    17641 cgcctggctg gcctgccagt cggcgtacca tgcccatgcc gtgaggaagg cgtcgccatt
    17701 gccatcttca ccccaccagg taaagcgcgc tccgggcccc gccagttgca tcagccgcag
    17761 acggttatcc tgaatcatct gacgaatgtc gttagcggcg gcactttgat gatcggcaag
    17821 cgaacgccag gcgagactga gcgggatcag acggctaccg gtgttgatca cgccacccca
    17881 cggttcatcg actaacgcat caagattgtt acggaaaatc tcctgcggcg tttcgctact
    17941 ttgtagccgg atattgctcg cctgctcggg caacatcagc gcattatcgc caccgccgag
    18001 catgacattt ttctgttgtt caacgggcca cctgttatcc acaaaagaca gttttgtgct
    18061 aatgctgtcc tgtacttgcc cattttgttg cagttctgca cttaacaagc cagattgctg
    18121 aatattctgc gtcagcgaaa tataattcgc ccctttgtgc agcgtcagcg tctggcgcat
    18181 ctcagcacct gcaaatttag tcaccagcgc caccggttcg ttatcctgct gactgaagat
    18241 aaacagtcct gccgccggtt tgtcacccat gcgatatacc gttggcatac tccacttcat
    18301 gtagagattt ttttccgaac gcaaataagc acgcccctgc ccgaccagcc cgtcgccgtt
    18361 catcccacgc gcggtgatgc gccagcgggt taacgaatca ggcatcagga acgtgaaata
    18421 cgctttgcct tgtttatcgg ttgtgagtga cggcatccag gccgcggtat ccacctcttc
    18481 acgccgtgga cgttccagca tttttactcg ccgctcgctg cggttagtcg cgccaggcgc
    18541 aaccggctcg ctggagagcg cctgatcgta gctgataaac gacagactgg agctggtacg
    18601 cacattgtta cgccccagcg gatagaaaaa tttgccgata ttcggcgcga tttccggttg
    18661 cagcgcgtag atcatttcat cgaccacgcc taccgttaac tgcgcagaaa caggtttacc
    18721 tttcagcgac gaggttaatt cgacattgac cagttcacca ggctggtaat gggttttgtc
    18781 cgttttcacc cggatatcca gctggggaac ggcaacttta atcccggcgt tctgaaaact
    18841 gtactgaccg ttacgggtat acagcaccga aaaagtgatg ttaggcgcaa aggaattgct
    18901 caccggtacg cgggcttcat actgggtatc gtttaaacgt tgtagcgtta gccagtttgc
    18961 cggatgcgat agcagcgact gctgttccac gcgatcgcgt tccagcgtca ataatgcttc
    19021 gtcaattggc tccgggaagg taatcagcat cttcgcggtt tcgccaggct gatacagtgt
    19081 tttatctgcc acgatatcta ccgtaccact atgcgacgtg ctgtccttac cgctgactgc
    19141 atggcttaac ccggcgagaa ttaagccgtc tttatcgcgt aatgtcagat tgtagttgcc
    19201 aggtttagcg aaattgacgg taaaggattt gccgcctgac tgtagctctc cgctgtgact
    19261 cgtgcggtct tcgagacgca accattcata cgtaacagga acctgttttg aagattccag
    19321 cgcggcataa cggaacacaa ccgactcgcc gctattactg tattgtgcgg cggtacttaa
    19381 agagtaatgc gccaggccac gttcaatgag gatctctttg gtggtggtaa ctcgatacgc
    19441 cgcgccgtca ctggcggaga cggttaacaa ataacggctc ggtttatcgg cggcggggag
    19501 attcaacgcc acatgaccat tatcgtcgga aaccgtttca ctgccttcca tcgacaccgg
    19561 gaaacgtccg gcataacgca aatcgttacc gaccattgat aattgctgag cgcgcaaact
    19621 taactgcacg cgggcatctt ttaccggctc gccatccggg taaagcagtt gcagtttgcc
    19681 gctgaccgct tcgccagttt tgaactcttt tttgtcgaga gctaaaccaa tctcaaaatg
    19741 gggtttgatg tagtttgcca cgcgaaaact gctgctatag acctgtttgc ggtaagcaag
    19801 acgtaactca taacctccgg cgacggcatt ttctggcagg cggaaactcc cctgcccgcc
    19861 attacgcgca tccagcgtga cattgacggt ttgcaacaga ctgccgttgg cgtccagcac
    19921 cgaaagcttc gccggtgcgc tgacgatggg ggatgaatgc aacggatcgt ggaactcgcg
    19981 acccatcact ttaacatcga cacgatcgcc tgcgcgatat agcgggcgat cggtaaagat
    20041 atacaagcgg gtgttgtaga tttcgctttc gtagaaaaag ttctcggaga caaaaacgcc
    20101 gccttcagca tccttaccca gaatgtatga acgttctggc gatatatgtt gtaactgcaa
    20161 ggtaccgctg ccatcggtca caccgcgggt catcacgcca agaccgtcag tccacaagat
    20221 ctctgagccg ggcttcgctt caccctgttt tttccccgcg gtccacacca gaagctcgtt
    20281 acctgacact ttgctaagcg ccacggtatc ggaaacaaac accaccgtcg tcgcccgata
    20341 cccacccacc atcgcttcga cgagatacag tcccggctct tgtttgccga gaggaatata
    20401 gatgttgccc ggctgcggcg agatgaaatt gctggatgcg ccttccagtt ttaccccttg
    20461 ctgcggctcg accggttttg cctgccatag tggatagcgg aactgttcca caagtggata
    20521 ttttttcagc ggtgaaaact ggctgttctg tacataacgg gaaggtttaa caatggcatt
    20581 accgagctgt aattcgggta acgcctgagt cacattctgc cgtgactgag aagagaaagt
    20641 acgctgcatc acgcggcgag atttgccgta ccagttatcc cacagccagg tcagcgtatt
    20701 gttcagtccg tcgcccagat attgcggttg caccacaatg cgatgcaggt ttttctgctg
    20761 gcgcaaaaat gccatcgggt caggaatacg atacaggcga acgtccacgc cgccgtactc
    20821 ttccatctga tagcgccgat aatcacgccc cggcgcttcc agtcgcactt tcgcctcttc
    20881 actgctgcta aaactgctgt cagcgagcaa aaagaatgtt cccccggcgg gcggcacata
    20941 gttactggaa ggaagcgaat cctcagcatt agcaagccct gttcccacaa gagacaggga
    21001 caagcacgct gctatcgcgc cagaaaattt aaacgataaa tgccaatgaa attgggattg
    21061 gaatcgttgg gtatccatcg ggtgtccttc catgtcataa gttgttgcag actgactgcg
    21121 cgcattccgt tgtcagtttt cgtggcgctt ccggtgtggt agatgatgta acgccccatc
    21181 cagaccatta agtgctgggc atcgccctga tcgaaaaaaa tcatatcgcc aggcagtgcc
    21241 tggtttatgt cctggccaat aaactggctg ttgtactgaa tcaaattaat cgcggtcaca
    21301 taggggccgg ttttcccatt cccctgattc cagttttgcg ccagttgacg ctgttcaggt
    21361 gttagcgtca tctctggcgg caaatactgg ttagataaac cgttactttt tagccattta
    21421 ctgtcgtgaa ctttcagcgc ctcgttcgcc gcaaatcgca ccaggcccgc acaatcctgc
    21481 tgataccagc gtgggcttgg cccctggcgg agctgttcct gtgcaatgcg cacaaaccac
    21541 gcgcgaaaca gcccggattg ttcgacattt agcatttcgc tatgggcaac aacacaatac
    21601 agccagcaaa tcagcgccag cagcccgtgt ctcatagcgg ttgccaggtt atgggtagcc
    21661 actgccaggc gacaccgggt tccatctggg ccagcttcat gacataacgc ggttgttgag
    21721 ataaagcgtc cagcttcggc attaataaag tttgtgcggc gttataaaaa accggttcga
    21781 gattcttcgg cagactggtc agcgtttcgt tacgcagcag tttcgccatt ccttgtggat
    21841 tgatatagag cggaacgatg ccatcagtgg gtattacatc caccattgcc gggcgggttt
    21901 tattcagtgt ttgcagggcg ttattaacca gcgtgtcatc gagggagaaa agcagcgttt
    21961 tgttttgcat cgccagcgac acgcggaaaa aataatccga cattaattga tcgggttgcg
    22021 ccgcctgcgc tttcggatac tgaccgtatc gggaactcac ttcgcgacgc cagatttgcg
    22081 cttcgccctg ctgagtctgg cttaccggca aaacaccttc tggcgctttg ctttcgtgcg
    22141 caccaatatt ttgcgtaaac agtttccctg gcaattgcgc ctgttcggca gtgccatcaa
    22201 actgaccaac aaacagtggg gtttgcaatt ttgagtcttc ataccagcac agccccgcgc
    22261 caccgtctaa cgccccattc agcttgtcgt tttcctggct gatgtgcgaa agcatctctt
    22321 cggcaatacc gtgtgaatac ggcaccgcca cacagaagct ggctccggca ggcatactgt
    22381 tccataccgg cgtaaaatcg aaactggcat ctacgctggc ggattcatcg tttaacgcca
    22441 gaaaactgtg ccagccgtcg ttacccattt cgaagcgtac gccagcaaaa gaaggcatta
    22501 atcgctggta gccaaacccc agcaacctgg cgctgactac gatgcgctgg cgtactggcg
    22561 ttttttcggc agtacgcgct tccaggccaa agcgggtttc ccagcgtttt ttaccactca
    22621 acaaatcact tgcgatcgcc gtggcttcgg tatcctgttg gtcatcttta aacagcatat
    22681 ccgaactgga aaaagccaat attttgtcct gataagtcgc aaacatcagg acgttattgc
    22741 cgttatagcg caactgataa acaggaatag tttcactatt tatcttgatg ctactgattt
    22801 ccgttttact taactggctg tcgctggtag cggcaaacag caatggctcc agcagcttgc
    22861 ttaaaccact tcgctggatc agcaccatat aatgagaaag atggccttgt ttatcgtgcc
    22921 acagtgccgc ctgcgcgggc tggtctaaga gtgacgaaaa gagcttatct ttcagcgtga
    22981 gatcgtgttc atagacaatg cggcgaatgc tgccttcaat gcccagacga tcggcatgat
    23041 tctgataata gaaaacgaaa tcttcgctca gaacatcgtg gagaaacgga atggtaagga
    23101 gatctttggg aagctggctc agagagtcgc tgtcgagaaa gaggtccggc tcattgagat
    23161 cgatttgcag attgttgtgc accaccagtg gcgacaacgt tttttctggc ccactgccag
    23221 agtattgtaa tgcccatacg ccagcggata gcagcgctat tgccccaaaa ccaacaaggc
    23281 cataaaaccg ccagcctttc gccttttttt caccactcat tgccacattc cttgtgtata
    23341 gccagccatt ttttacgggc acagccaaac tttaccgcgc cctaatacga caaaagccca
    23401 gactttacag cctggacttt tcaattcaaa aaagggagat ggctcccttt tggcatgaag
    23461 cgatgaaatt attcttcttc tggctcgtcg tcaacgtcca cttccggagc gatttcatcg
    23521 tccccttccg cggcactgcc gtcgatggta tccagatctt cctcgtcaac cggttcagca
    23581 acacgttgca gacccaccac gttttcatct tccgaagtac ggataaggat tacgccctgg
    23641 gtgttacggc ctacgatgct gatttccgac acgcgagtac gtaccagcgt accggcgtcg
    23701 gtgatcatca tgatctggtc gcaatcatct acctgcaccg cgccaaccac taaaccgtta
    23761 cgttcggtaa ccttgatgga gataacccct ttcgtcgcac gcgacttggt tgggtattcc
    23821 gccactgcgg tacgtttacc ataaccgttc tgcgttgcgg tgaggattgc gccatcgcca
    23881 cgaggcacga tcagagagac gactttatcg ccttcgccta aacgaatacc acgaacaccg
    23941 gtggtgttgc agcccatcgc acggacagaa gactctttaa agcgcaccac tttaccttca
    24001 gcggagaaca gcattacttc gtcttcgccg ctggtcaggt caacgccgat cagctcatcg
    24061 ccgtcaacca gtttgatcgc cactttaccg gcggtacgca gacggttgaa ctcggtgagg
    24121 acggttttct tcacagtacc gttagcggtc gccatgaaga ctttcacgcc ttcttcaaac
    24181 tcggtcaccg gcaggatcgc ggtgatacgt tcgtcctgtt ccagcggcag caggttgacg
    24241 atcggacgac cacgtgcgcc acgagtagct tctggcaact ggtagacctt catcgaatag
    24301 acgcggccac ggctggagaa gcacagaata tggtcgtggg tgttcgccac cagcagtcgg
    24361 tcaataaagt cttcttcttt aatacgtgcg gcagatttac ctttcccgcc acgacgctgc
    24421 gcttcgtatt cagaaagcgg ctgatacttc acgtaaccct ggtgagaaag cgtgaccacc
    24481 acatcttcct gtgtgatcag atcttccagg ttgatgtcgg cgctgttagc ggtgatttcg
    24541 gtacgacgtt tgtcaccgaa ctgttcacga accagctcca gctcttcacg gatcacttcc
    24601 atcagacgat cggcgctacc cagaatacgc aacagctccg caatctgatc cagcagctct
    24661 ttgtattcgt cgagcagttt ttcgtgctca aggccggtca gtttctgcaa acgcagatcc
    24721 agaatcgcct gagcttgctg ttcggtcagg tagtacagac catcacgcac gccgaactct
    24781 ggctccagcc attccggacg cgcagcatca tcgcccgcac gttccagcat cgcagcaacg
    24841 ttgcccagct gccacggatt agcaaccagc gcagttttcg cttctgcagg cgtcggcgca
    24901 tgacggatca gttcgatgat cgggtcgatg ttcgccagcg ccacggctaa tgcttcaaga
    24961 atatgagcac gatcgcgagc tttacgcagt tcgaaaatgg tacgacgggt caccacttca
    25021 cggcggtgac gaacaaacgc cgcgatgatg tctttcaggt tcatgatctt cggctgacca
    25081 tggtgcaatg ccaccatgtt aataccgaaa gaaacctgca actgggtctg ggagtagagg
    25141 ttgttgagca ccacttcacc gaccgcatcg cgtttgactt caatcacgat gcgcataccg
    25201 tctttgtcag actcgtcacg cagcgcgctg atgccttcca cgcgtttttc ttttaccagt
    25261 tccgcaattt tttcgatcag gcgcgctttg ttcacctgat acggaatttc gtggacgata
    25321 atggtttcac gtccggtttt ggcgtcaact tctacttcgg cacgggcacg gatatatacc
    25381 ttgccgcgac cggtacggta agcttcttca ataccgcgac gaccgttaat gattgccgcc
    25441 gtcgggaagt ccggccccgg gatgtgttcc atcagccctt caatgctgat gtcttcatca
    25501 tcgatatacg ccagacaacc gttgatgact tccgtcaggt tgtgcggcgg aatgttggtt
    25561 gccataccta cggcgatacc ggaagaaccg ttcaccagca ggttaggaat tttggttggc
    25621 atgacgtccg gaattttttc cgtgccgtca tagttatcaa cgaaatcgac cgtctctttt
    25681 tcgagatcgg ccatcagttc atgggcaatt ttcgccagac ggatttccgt ataacgcatt
    25741 gccgccgcag agtcgccgtc gatggaaccg aagttaccct gaccgtctac cagcatgtaa
    25801 cgcagcgaga atggctgcgc catacggacg atcgtgttat aaaccgccaa gtcaccatgg
    25861 ggatggtatt taccgattac gtcaccaacg acacgggcag attttttata ggctttgttc
    25921 cagtcattgc ctagtacgtt catggcgtaa agtacgcgac ggtgtaccgg cttcaggcca
    25981 tctcggacat ctggcagcgc acggccaaca atgaccgaca tcgcataatc cagataggag
    26041 ctcttcagct cttcctcaat gttgaccggt gtaatttctc tcgcaaggtc gctcatctaa
    26101 ccgctatccc tctactgtat cccggattca aaggtcgcaa attataacac agccgcgcag
    26161 tttgaggtaa acctatacgc tttattcaca tccaatgcct gatatactcg tttgtcttgc
    26221 caattacgga gtagaagtgc caatgaatgc cgaaaaatcg ccggtaaacc acaacgtaga
    26281 ccacgaagag atcgctaaat ttgaagccgt cgcctcccgc tggtgggatc ttgaaggtga
    26341 gttcaaaccg ctgcaccgca ttaacccgct gcgtctgggc tatattgccg agcgtgctgg
    26401 cggtttattt ggcaaaaagg tgctcgatgt cggttgtggc ggcggcattc tggccgagag
    26461 tatggcgcgg gagggcgcga cggtgaccgg tctggatatg ggctttgagc cattgcaggt
    26521 ggcaaaactg cacgcactgg aaagcggcat tcaggtggat tacgtgcagg aaaccgtgga
    26581 agagcacgcg gcaaaacatg ccggacagta tgacgtggtg acctgtatgg agatgctgga
    26641 gcacgtcccc gatcctcagt cagtggtcag agcctgtgcg caactggtga aaccgggcgg
    26701 cgatgtcttt ttctcgacgc ttaaccgcaa cggcaagtca tggctgatgg cggtggttgg
    26761 tgctgaatat attttgcgca tggtgcctaa aggtacacat gatgtgaaga agtttattaa
    26821 accggcagaa ttgctgggct gggtggatca gaccagtctt aaagaacggc atatgaccgg
    26881 gctgcattac aaccctatca ctaacacctt taagctcggc cccggcgtgg atgtgaacta
    26941 tatgctgcat acgcagaata agtgaggttg ttgtttggat tcatccggca accgcgccaa
    27001 gcctgatgcg acgcttgccg cgtcttatca ggcctacaaa tgctccccgt aggccagata
    27061 aggcgtttac gccgcatccg gcaacagtgc cggtcagaca gcgattacca tttcactgtc
    27121 atcgacaaaa accctgccat ctgggcaaaa tcatcactcc ctttctgcca cgccacgctg
    27181 ccgcgcagcg aaactcgctg actgatattg cccgttattc ccacttttat ttcaccccgt
    27241 tgcttcaccg catcgtcact gatagtgctg ccatcctctt caatttccgt cgcatgggga
    27301 tcgtgataat aattcagatc taacgttggc gtgacaccaa cagcagtacg ccattcgctg
    27361 tgtaaaccca accgcatctg aatatcatca ccctgcgatt gtgacacgcg cgcatggtta
    27421 gcggcggtaa aatcatcctg ctgcacgccc tgataaatca cctgcgcctg cggttcaatc
    27481 accacaccac gccccggtaa ccactgataa ccggcttcca gcgaggcgat aatccccgac
    27541 gagtggtaat gatccactcc atcgtcttgt tcggaaacat cattgttaaa ccacgcatat
    27601 tgcagccagc tatccaacca ggccccctgc ttctgattac cgtgctgata ccagcttgag
    27661 gtcagcccaa cggcataacc gcggttctgg ttatcggcgc gagttccggt catattcgag
    27721 cggctgtcgc cctggttatc gctgtagcca ccaacaatcc caagcatcca ctcgccatca
    27781 tcaccccagt gcccgctaaa caggttgccg ctaagctgca ccgtagaagt gtcttcatgc
    27841 tgagccaact gccctgccgc cgtgtaatga taacgcccgc cgataacgcg taaattcagc
    27901 gtctgaccat cgccacctgc gtgatcgcgt cgctccatca caaatgcctg atttgccgca
    27961 cgtaggttat taaaatatcc accaactttg gcattcaaca ccggctggta agcaggcgta
    28021 ggttcagggt cgggggttgg gtcgggatcc ggcgtgggat caggatctgg agtcgggtct
    28081 ggatcaggcg gcggcgtcgg attgacttct tgtgatcgca gataccagtc gttgttatct
    28141 tccaccagcg tgtagtcata cgcccccata ttgacgtagc cgcttcctgc cagactgaac
    28201 tgcgcattgt tttgaaactg cgtgggatca gctgcgaaat caaccacttt aatacctgta
    28261 gatgtcggct caccaatccc tgtaatggga ttaatcacaa ccgtggtatt gccagcagta
    28321 ttaccgttca acaccaactg gtcgcttgcc gagtcatcgc cgtttaattc gctgtcgagc
    28381 agtagcgtac cgccgccggt ataatcacca tttaccgtca gcgtgtcgcc agcgatgcca
    28441 ttttgcagac tgacagcacc ggacgtattg gtcagattgc cattaaccgt cacgtcggaa
    28501 agataagtca ggctgtcagt ttcaaggttg gtattaagct cacctgtcaa cgtcacgcca
    28561 tcggtggcat tcaccagcgt accgtcgccg ctaaacagcg aggcatccag cgcctggttc
    28621 gtatcctgct gcgtcaatcg caacacagta ccatcggtta tgtcgatatc gccgccagag
    28681 gtggcatcga ttgagcgaat atcctgattt tgcccggttt caaaaacacc gtcttccccc
    28741 acccacagtg aagaaacata aggaataata ttctccgctt caccttttag cgttccgtct
    28801 tccacacgta ccgcagacgt cgcagtgccg ctggcggtca actccagcgt cccgccgcca
    28861 cttttagtca acgtgcttac gctatccagt tgttgcccgg tactatccgc catcaacccg
    28921 ccccattgcg tatctacgcc atcctcaacg tgtatttcgc catcagcgcg catttcaata
    28981 ttcctgccct gacctgttgt agccgagcga gtgtcaacat cagtcgtcac atccatttca
    29041 ctgtgttgtt ggggatctgt gaagatcact gaacggttgt aacttgtgtc ccccaattgc
    29101 gaattatcgc tgacttcaag cgtaccggag gcgatttgcg tattaccgag ataaccgttg
    29161 ttattcgcca gcgttacgcg ctgatccccc gcattaatga cgacataaac gccattctgg
    29221 ctggcatcac tgccgccaat atgtaaatca tcgccactgc tgatatcaac gacatcatta
    29281 ctgccaatca ctgtcccccc gctgccgctg atttgcagac cgtcattgta tgagctatcg
    29341 ccatcccagg tggtgaaatc agagagatcc aatactccgc catttagcac gatcgattgc
    29401 ggatcgctct gcattgcccg taaatcggcc tgattccccg ccagcgacaa tacggcgtta
    29461 tcttccacca caatatcgcc ggttaatgcc atcgactgcg cccctgtcag gagatagctg
    29521 ccatcttgcg ctaccgtcaa ctgtccttca ccctgaatcg agccggaaaa accgccctgg
    29581 tttacggtaa cattgccgcc tgcgtcgata ttcaaaatgc cattagcaaa accggttaat
    29641 gagtgcacaa acgtttgttg tgtattgcca acattcagtt ctgcctgata ctcagaatga
    29701 acagtgctgc cgaccatcag accaaagcaa tcctgcggat cgctctgaca gtgcgtgtcg
    29761 ccgacattca ttaactcatc gctgcgcccc aacgtcacct cgccattctg gatactcatc
    29821 tcgccggtaa acgcattatt gtcggcattg aggactaact ctcccgctcc ctctttcacg
    29881 atgactcccg ttccggcaag agaatcaatc gcgccatcgt tgctggtatt gcctattacc
    29941 agcgttttat cggcggcaat gttaaattca gccacactat gaccgatata cataaagccc
    30001 cccgccgctg ccgtcgccga atggtcatat cccgaggcgc tgttattttc atcaatcatc
    30061 acaccattgt tttggtcata attatcatca acagaaatat caataagata cggggatgtg
    30121 gtgctgctgg tataaattgc tccgccataa ccttctgcat agttattggt aaaggaggta
    30181 ttattaatta tcgtttttcc tgaaagatgt gtcttatccg tactattatc cgtaacatca
    30241 atggcgccgc catccccatc actataactt gttgatgtat atgcctggtt attattaaaa
    30301 gcatcatcac ttaaatagac gtcattattg atggtataaa ttgcaccgcc tttgccgtca
    30361 ttagcgatgt tattgtgaaa tacggcgtta gtgactcgta aatcggcggc accggtatca
    30421 ttggtaccgg cagaatagat tgctccgcca tagcctcctg caacattact gtcgaaaata
    30481 acattcgcaa gatttatcgt tgagttttct ttagcaaaga tagccccgcc attattatat
    30541 tcgccactaa ccgagttatc agcaaacagg gtcatccctg tttcattttg tggtgatatc
    30601 gtaaattcag cactgtgctg caaaaatact gcgccaccgc tggcattatt gaccataccc
    30661 gaaaaaatca accattgtcc atccgcgatg ctccagtcct gtatattacc cgaaaggctt
    30721 tgcctgctgg cctgacagct cgctttgata tcatatccct ggcatgaatc aatgaccgct
    30781 gcggcaccgt tagcagagaa aagagatgca atcattgacg ggagtaaaga taaatactcc
    30841 ttgcgtagag agataatccg catattcatc taaccatcat tttctataag atggcgtatt
    30901 taatcgcatt atgcgatata gggtattttt cttttgtaaa ttctgccccc gat
//
LOCUS       DACADF010000045        30934 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.045, whole genome shotgun sequence.
ACCESSION   DACADF010000045 DACADF010000000
VERSION     DACADF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.045"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            369..2000
                     /gene="oppA"
                     /locus_tag="ISB30_001929"
     CDS             369..2000
                     /gene="oppA"
                     /locus_tag="ISB30_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAP1989564.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRAALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2086..3006
                     /gene="oppB"
                     /locus_tag="ISB30_001930"
     CDS             2086..3006
                     /gene="oppB"
                     /locus_tag="ISB30_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAP1989565.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3021..3929
                     /gene="oppC"
                     /locus_tag="ISB30_001931"
     CDS             3021..3929
                     /gene="oppC"
                     /locus_tag="ISB30_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAP1989566.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3941..4954
                     /gene="oppD"
                     /locus_tag="ISB30_001932"
     CDS             3941..4954
                     /gene="oppD"
                     /locus_tag="ISB30_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAP1989567.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMDICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4951..5955
                     /gene="oppF"
                     /locus_tag="ISB30_001933"
     CDS             4951..5955
                     /gene="oppF"
                     /locus_tag="ISB30_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAP1989568.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6008..6337)
                     /locus_tag="ISB30_001934"
     CDS             complement(6008..6337)
                     /locus_tag="ISB30_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAP1989569.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6372..7832)
                     /gene="cls"
                     /locus_tag="ISB30_001935"
     CDS             complement(6372..7832)
                     /gene="cls"
                     /locus_tag="ISB30_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP1989570.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7975..8148
                     /locus_tag="ISB30_001936"
     CDS             7975..8148
                     /locus_tag="ISB30_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAP1989571.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8203..9456)
                     /gene="kch"
                     /locus_tag="ISB30_001937"
     CDS             complement(8203..9456)
                     /gene="kch"
                     /locus_tag="ISB30_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAP1989572.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILSKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9756..10052)
                     /locus_tag="ISB30_001938"
     CDS             complement(9756..10052)
                     /locus_tag="ISB30_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAP1989573.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10276..10992
                     /gene="tonB"
                     /locus_tag="ISB30_001939"
     CDS             10276..10992
                     /gene="tonB"
                     /locus_tag="ISB30_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAP1989574.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            complement(11032..11430)
                     /gene="yciA"
                     /locus_tag="ISB30_001940"
     CDS             complement(11032..11430)
                     /gene="yciA"
                     /locus_tag="ISB30_001940"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAP1989575.1"
                     /translation="MSTAHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11535..12074)
                     /locus_tag="ISB30_001941"
     CDS             complement(11535..12074)
                     /locus_tag="ISB30_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAP1989576.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12104..12847)
                     /locus_tag="ISB30_001942"
     CDS             complement(12104..12847)
                     /locus_tag="ISB30_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAP1989577.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(12875..12964)
                     /locus_tag="ISB30_001943"
                     /pseudo
     CDS             complement(12875..12964)
                     /locus_tag="ISB30_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13203..13841
                     /gene="ompW"
                     /locus_tag="ISB30_001944"
     CDS             13203..13841
                     /gene="ompW"
                     /locus_tag="ISB30_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAP1989578.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAASPFRHKVGTATTGDIATVHHLPP
                     TLMAQWYFGDSGSKFRPYIGAGINYTTFFNNDFNDTGEGLGLSDLSLKDSWGVAGQVG
                     LDYLINRDWLLNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            complement(13887..15017)
                     /locus_tag="ISB30_001945"
     CDS             complement(13887..15017)
                     /locus_tag="ISB30_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1989579.1"
                     /translation="MARPRKYKTNVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(14995..15243)
                     /locus_tag="ISB30_001946"
     CDS             complement(14995..15243)
                     /locus_tag="ISB30_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP1989580.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            complement(15308..17779)
                     /locus_tag="ISB30_001947"
     CDS             complement(15308..17779)
                     /locus_tag="ISB30_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP1989581.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGSQQNTDASDTTAQ
                     PDLVKVTADEVNKIMQAASISQPDANKLLAASRGEFVEGISDPNDPKWVKGIETRDSV
                     HQNQSETEQYDQKAEQNSQNALQNEPETKQPEPVVQQEEEKVCTACNQTGGGNCPDCG
                     AVMGDATYQETFNEESQDEAREKDPEEIENAGLPNKECTEGDQHANDNNEIGETANPL
                     IKVNSHRKSTSTSRLWHHLMIDLETMGKNPDAPIISIDAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDVIKRWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFENTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPNQADF"
     gene            complement(17872..18063)
                     /locus_tag="ISB30_001948"
     CDS             complement(17872..18063)
                     /locus_tag="ISB30_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HAP1989582.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            complement(18060..18248)
                     /locus_tag="ISB30_001949"
     CDS             complement(18060..18248)
                     /locus_tag="ISB30_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HAP1989583.1"
                     /translation="METLLPNVNTSEGCFEIGVNISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            complement(18814..19032)
                     /locus_tag="ISB30_001950"
     CDS             complement(18814..19032)
                     /locus_tag="ISB30_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989584.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARTKQAE
                     NLSALEQDITRLEKAAALSIARMVTYPR"
     gene            complement(19192..19347)
                     /locus_tag="ISB30_001951"
     CDS             complement(19192..19347)
                     /locus_tag="ISB30_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAP1989585.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(19620..20336)
                     /locus_tag="ISB30_001952"
     CDS             complement(19620..20336)
                     /locus_tag="ISB30_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1989586.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            20386..20601
                     /locus_tag="ISB30_001953"
     CDS             20386..20601
                     /locus_tag="ISB30_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1989587.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
     gene            20598..21023
                     /locus_tag="ISB30_001954"
     CDS             20598..21023
                     /locus_tag="ISB30_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAP1989588.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            21046..22008
                     /locus_tag="ISB30_001955"
     CDS             21046..22008
                     /locus_tag="ISB30_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1989589.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGPMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASYDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            22015..22761
                     /locus_tag="ISB30_001956"
     CDS             22015..22761
                     /locus_tag="ISB30_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP1989590.1"
                     /translation="MKNIAAVGVLERIRRLAPQGSVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMVKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            22783..23553
                     /locus_tag="ISB30_001957"
     CDS             22783..23553
                     /locus_tag="ISB30_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HAP1989591.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRKGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            23569..23994
                     /locus_tag="ISB30_001958"
     CDS             23569..23994
                     /locus_tag="ISB30_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAP1989592.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRLSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNVRYFRRY"
     gene            24169..24834
                     /locus_tag="ISB30_001959"
     CDS             24169..24834
                     /locus_tag="ISB30_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP1989593.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            25015..25227
                     /locus_tag="ISB30_001960"
     CDS             25015..25227
                     /locus_tag="ISB30_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP1989594.1"
                     /translation="MSHKCRLASYVPKGKGKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRLRTGQTEVAVFTAYEPEE"
     gene            25395..25667
                     /locus_tag="ISB30_001961"
     CDS             25395..25667
                     /locus_tag="ISB30_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989595.1"
                     /translation="MAHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            25669..26724
                     /locus_tag="ISB30_001962"
     CDS             25669..26724
                     /locus_tag="ISB30_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAP1989596.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAHQNLFAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            26725..27105
                     /locus_tag="ISB30_001963"
     CDS             26725..27105
                     /locus_tag="ISB30_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP1989597.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     GQPVSGGRLGVKIYPIMHEEQVKK"
     gene            27102..27923
                     /locus_tag="ISB30_001964"
     CDS             27102..27923
                     /locus_tag="ISB30_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAP1989598.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCRGEKFIEGEVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKIREVVKVSCPECKGKGEISTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
     gene            28150..28347
                     /locus_tag="ISB30_001965"
     CDS             28150..28347
                     /locus_tag="ISB30_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAP1989599.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATAGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            28499..29548
                     /locus_tag="ISB30_001966"
     CDS             28499..29548
                     /locus_tag="ISB30_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAP1989600.1"
                     /translation="MANTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLVDTYTSLNRQYVELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRAIGVELET
                     GRFEQTVREVQGLIV"
     gene            29598..29673
                     /locus_tag="ISB30_001967"
     tRNA            29598..29673
                     /locus_tag="ISB30_001967"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29631..29633,aa:Ile,seq:cat)
     gene            29774..29850
                     /locus_tag="ISB30_001968"
     tRNA            29774..29850
                     /locus_tag="ISB30_001968"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29808..29810,aa:Arg,seq:tct)
     gene            30350..30481
                     /locus_tag="ISB30_001969"
     CDS             30350..30481
                     /locus_tag="ISB30_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAP1989601.1"
                     /translation="MIAYCFCEWHCCSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(30762..30917)
                     /locus_tag="ISB30_001970"
     CDS             complement(30762..30917)
                     /locus_tag="ISB30_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001450624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene biosynthesis protein"
                     /protein_id="HAP1989602.1"
                     /translation="MYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQICPY
                     GLKQQSP"
BASE COUNT         7916 a         7188 c         7875 g         7955 t
ORIGIN      
        1 gatgcaagct atagattctg gtacggtcaa taaaagagaa ttgcttaaca attttgcaaa
       61 atgtattggc gagtaagaac cgcatttggt actttccggg caaccgccag gcgattcttt
      121 attggtaatg agaataatta acaattaaag accgtcgcga aagaataatg tgtctcgaca
      181 ggggagacac agtacgaatc gacataaggt gatcgtctga atcaccagaa taaataaagt
      241 cggtgatagt aatacgtaac gataaagtaa cctgacagca gaaagtctcc gagcctgtgc
      301 agggtcccaa tccgggatta cacatgctgg ttaataccag taattataat gagggagtcc
      361 aaaaaacaat gaccaacatc accaagagaa gtttagtagc agctggcgtt ttggctgcgc
      421 ttatggcagg gaatgtcgcg ctggcagctg atgtacccgc aggtgtcaca ctggcggaaa
      481 aacaaacgct ggtacgtaac aatggttcag aagttcagtc attagatccg cacaaaattg
      541 aaggtgttcc ggagtctaat atcagccgag acctgtttga aggcttactg gttagcgatc
      601 ttgacggcca tccggcacct ggcgtcgctg aatcctggga taataaagac gcgaaagtct
      661 ggaccttcca tttgcgtaaa gatgcgaaat ggtctgatgg cacgccagtc acagcacaag
      721 actttgtgta tagctggcaa cgttctgttg atccaaacac tgcttctccg tatgccagtt
      781 atctgcaata tgggcatatc gccggtattg atgaaattct tgaagggaaa aaaccgatta
      841 ccgatctcgg cgtgaaagct attgatgacc acacattaga agtcacctta agtgaacctg
      901 ttccgtactt ctataaatta cttgttcatc catcaacttc accggtgcca aaagccgcta
      961 tcgagaaatt cggcgaaaaa tggacccagc ctggtaatat cgtcaccaac ggtgcctata
     1021 ccttaaaaga ttgggtcgta aacgaacgaa tcgttcttga acgcagcccg acctactgga
     1081 acaacgcgaa aaccgttatt aaccaggtaa cctatttgcc tattgcttct gaagttaccg
     1141 atgtcaaccg ctaccgtagt ggtgaaatcg acatgactta taacaacatg ccgatcgaat
     1201 tgttccagaa gctgaaaaaa gagatcccgg acgaagttca cgttgatcca tacctgtgca
     1261 cttactatta cgaaattaac aaccagaaac cgccatttaa tgacgtgcgt gtgcgtaccg
     1321 cactgaaatt gggtatggac cgcgatatca ttgttaataa agtgaaagcg cagggcgaca
     1381 tgcccgccta tggttacact ccaccgtata ctgatggcgc aaaattgact cagccggaat
     1441 ggtttggctg gagccaggaa aaacgtaacg aagaagcgaa aaaactgctg gctgaagcgg
     1501 gttataccgc agacaaaccg ttgaccatca acctgttgta taacacctcc gatctgcata
     1561 aaaagctggc gattgctgcc tcttcattgt ggaagaaaaa cattggtgta aacgtcaaac
     1621 tggttaacca ggagtggaaa acgttcctcg acacccgtca ccagggcact tttgatgtgg
     1681 cccgtgcagg ctggtgtgca gactacaacg aaccaacttc cttcctgaac accatgctgt
     1741 cgaacagctc aatgaatacc gcgcattata agagcccggc ctttgacagc attatggcgg
     1801 aaacgctgaa agtgactgac gaggcgcagc gtgcagctct gtacactaaa gcagaacaac
     1861 agctggataa ggattcggcc attgttcctg tttattacta cgtgaatgcg cgtttggtga
     1921 aaccgtgggt tggtggctat accggcaaag atccgctgga taatacctac acccggaata
     1981 tgtacattgt gaagcactaa tggcaatacg tggggcagga gtgtcctgct ccacggtgtc
     2041 tgatttttat cgcattacag aaggcacagg ccagaaggta gggcaatgtt aaaatttatt
     2101 ctacgtcgct gtctggaagc gattccgacg ctatttattc ttattactat ttcgttcttt
     2161 atgatgcgcc tcgcgccggg cagtcctttt accggcgaac gtactttacc accggaagtg
     2221 atggccaata tcgaagcgaa atatcatctt aatgatccga tcatgacaca gtatttcagc
     2281 tacctgaaac aactggcgca tggcgatttc ggtccatcgt ttaaatataa agattattcg
     2341 gtcaatgacc tggttgcatc cagttttccc gtttctgcca aactgggagc tgcagcattt
     2401 tttcttgcgg taatactggg tgttagtgct ggcgttattg ccgcattaaa gcaaaacacc
     2461 aaatgggact ataccgtgat ggggctggca atgaccgggg ttgttatccc cagttttgtg
     2521 gttgcgccat tattagtcat gatatttgcg atcattttgc attggctgcc gggcggtggc
     2581 tggaatggtg gagcgcttaa attcatgata ttgccaatgg tggcgttatc actcgcctat
     2641 atcgccagta ttgcgcgtat tacccgtggc tctatgattg aggttttaca ctccaacttt
     2701 attcgtactg cccgggcgaa agggttacct atgcggcgga tcattttacg ccacgcatta
     2761 aaacctgctc tgttacccgt gctctcctat atggggcccg catttgtcgg cattattacc
     2821 ggttcgatgg tcatcgaaac catttatggt ttaccgggga ttgggcaatt gttcgttaat
     2881 ggcgcattga accgtgacta ttccttagtg ttaagtctga ccattctggt tggcgcgtta
     2941 accattttgt ttaatgccat tgtcgatgtg ctatatgcgg ttatcgaccc gaaaatccgt
     3001 tactgatact ggagctcgcg atgatgttaa gtaagaaaaa cagcgagacg ctggaaaatt
     3061 tcagtgaaaa gctggaggtc gaagggcgca gcttgtggca ggacgcacgt cgacgtttta
     3121 tgcataaccg tgcggcggtt gccagtctga tagtgctggt gctgatcgcg ttatttgtaa
     3181 tcctggcacc gatgctttcg cagtttgcct atgacgatac tgactgggcg atgatgtcca
     3241 gcgccccgga tatggagtcc ggtcactact ttggtactga ctcatccggt cgcgacctgc
     3301 ttgtgcgcgt tgcgattggc gggcgtatct cactcatggt cggtgttgct gcggcactgg
     3361 tggcagtggt cgtggggaca ctttatggtt cgctttccgg ttatctgggc ggtaaagtgg
     3421 attcggtaat gatgcgtctg ctggaaatcc tcaactcctt cccattcatg ttcttcgtca
     3481 ttttgctggt gacctttttc ggtcaaaaca tcctgctgat tttcgtggcg attggcatgg
     3541 tttcctggct ggatatggct cgtattgtgc gtgggcaaac cctgagtctg aagcgcaaag
     3601 agtttattga ggcggcacaa gttggcggtg tatcgacgcc gggcattgtt attcgccaca
     3661 ttgtgccgaa cgtactcggt gtggtggtgg tctatgcgtc gcttctggtg cccagcatga
     3721 tcctctttga atctttcctt agcttcctgg gattaggtac acaagagccg ttaagcagct
     3781 ggggtgcatt gctgagtgat ggcgcgaact cgatggaagt ctctccatgg ttactgttgt
     3841 tcccagcggg attcctcgtg gtaacgctgt tttgtttcaa ctttatcggc gatggcttgc
     3901 gtgatgccct cgacccgaaa gatcgttaag gagtgcagcc atgagcgtaa ttgaaactgc
     3961 aactgtgccg ctcgcacaac aacaggctga cgcactgctg aacgtgaaag atttgcgtgt
     4021 cacctttagt accccggatg gcgacgtcac ggcggtcaat gatttgaatt tttcgctacg
     4081 tgccggagaa acgctgggca tcgtaggtga gtctggttcg ggcaaatcgc aaaccgcatt
     4141 cgcgttgatg ggcctgttag ctgccaacgg acgtattggc ggatcggcaa ccttcaatgg
     4201 gcgtgaaatc ctcaatttgc cagagcatga actcaataaa ctgcgagctg aacaaatctc
     4261 aatgattttt caggacccaa tgacttcgtt gaatccctat atgcgcgtcg gtgagcagtt
     4321 gatggaagtg ctgatgctgc ataagaacat gagcaaagct gaggcgtttg aagaatcggt
     4381 gcggatgctc gatgcggtaa aaatgccgga agcgcgtaaa cgcatgaaaa tgtacccgca
     4441 cgaattttct ggcggcatgc gtcagcgagt catgattgcg atggcattgc tatgtcgacc
     4501 taagctgctg attgcggatg aacccactac tgcgctggac gtcaccgtac aggcgcagat
     4561 catgacgcta ttgaatgaac tgaagcggga atttaatacc gccatcatta tgattaccca
     4621 cgatcttggc gtggtggcgg ggatctgcga caaagtgctg gtaatgtacg ccgggcgcac
     4681 gatggaatat ggcaacgcgc gcgatgtctt ttatcaaccc gtacatcctt attctatcgg
     4741 tttgctcaac gcggtgccgc gtctcgatgc ggaaggtgaa acaatgttga ctattccggg
     4801 taatccgcca aacctgctgc gattaccgaa aggttgcccg ttccagccac gttgtccgca
     4861 tgcgatggat atttgtagta gcgcaccgcc gctggaagag tttacgcctg gccgtctgcg
     4921 tgcttgcttt aaaccggtgg aggaactgtt atgaatgctg taaccgaagg aagaaaagtc
     4981 ctcctcgaaa tcgccgatct gaaagtgcac tttgaaatca aagatggcaa acagtggttc
     5041 tggcaaccgc cgaaaacgct caaagccgtc gatggtgtaa ctcttcgcct gtatgaaggg
     5101 gaaacattag gtgtggtagg ggaatcggga tgcggtaagt ccacctttgc tcgcgccatc
     5161 attggcctgg ttaaagcgac cgacggtcat gttgcctggt taggtaaaga gttgctgggc
     5221 atgaagcccg atgaatggcg tgccgtacgc agtgatattc agatgatttt ccaggatccg
     5281 ttggcatcgc tgaacccgcg tatgaccatc ggcgagatca tcgctgaacc actgcgtact
     5341 tatcatccga aaatgtcacg ccaggaagtt cgcgagcgcg tgaaggcgat gatgctgaaa
     5401 gtcggtttgt tgcctaacct gattaaccgc tatccgcatg agttctccgg tgggcagtgc
     5461 cagcgtatcg ggattgctcg cgctcttatt cttgaaccga agctgattat ctgtgatgag
     5521 ccggtgtcgg cgctggacgt gtcaattcag gcgcaggtgg tcaacctgct ccagcagttg
     5581 caacgtgaga tgggattgtc attaattttt atcgctcacg acctggccgt ggtaaaacac
     5641 atttccgatc gtgtgctggt gatgtatctc ggccatgcgg tagaactggg gacctatgat
     5701 gaggtctacc acaatccgct acatccttac accagggcat tgatgtcggc agtccccata
     5761 cctgatccgg atctggagaa gaacaaaacc atccagttac tggaagggga attaccgtcg
     5821 ccgatcaacc cgccttccgg ttgtgttttc cgtacccgtt gtccgattgc aggtccggag
     5881 tgcgccaaaa cacgtcctgt gctggaaggg agtttcagac acgccgtttc ttgcctgaaa
     5941 gtcgatccgc tttaaaagca ataagggctg acagttgtca gcccttattc acgccaaaag
     6001 cgattattta ttcccgccag atgatatggc aaagtttgtg atctttttcg cggcataaca
     6061 gaatgcgcgc gaaaacatcg ttgatttcac catcttcact gtccgccaga ccaatcacca
     6121 cttcggcaaa aaagtccggg ttcaaatcga aatcaacatg ttcctgccag tcttccgccg
     6181 gatcgaataa ctccgcgccg ccgcgctctt caaactgaag attgaacagc agaacatcag
     6241 ctggatcgag attgtcggca gccagttcga gaaaaatatc gtaggcctgc tcaagcgttt
     6301 catcttcagt caggcgattg tttagatcca tatccatagt cactacctgt ttaacctctg
     6361 ttggcgacgt tttacagcaa cgggctgaag aagtaaaaca gtcgctcggc gacacgttgc
     6421 cataatggac gttttagcca taaacgggca tcgagcagac gagaacgcga aatataatcg
     6481 tcctgaacgg cggcgaggtc tgcaccaaaa cctttatcgt cgattgccag ggtaatctcg
     6541 aaatttagcc acagactacg catatcaagg ttaacggtgc caaccagact tagttctcca
     6601 tcgaccagca cgctcttggt atgcagtaac ccgccttcaa actgataaat tttaacccca
     6661 gcagccagca gttctgtaaa gaatgcgcga ctggcccagc cgaccagcat cgagtcattt
     6721 tttcgcggaa ggataatact gacatccacc ccgcgctgcg ccgccgtgca aatcgcatga
     6781 agtaaatcat cgcttggcac aaaatagggc gttgtcatga tcaaatattc acgagccgaa
     6841 taagcagcag tcaataatgc ctggtgaatg agatcttccg gaaagccggg gccagaagca
     6901 attgtgtgaa tggtgtgacc actggcctgc tcaaacggca taatattgac atctggtggc
     6961 ggcggcagaa tacgtttccc ggtttcaatc tcccagtcgc aggaataaat aatccccatc
     7021 gcggtggcga tggggccttc catacgcgcc atcagatcaa tccattgccc tacacccgca
     7081 tcttgtttga agtagcgagg gtcgaccata ttcatgctgc cggtgtacgc gatgtaatta
     7141 tcgatcatga tcatcttgcg atgttggcgc aggtccatac ggcgtaaaaa cacacgcatc
     7201 agattgacct ttaaggcttc gacaacttca ataccggcat tacgcattag ctcgggccac
     7261 gggctgcgga aaaaagccac actcccggcg gagtcgagca tcaatcggca atgaataccg
     7321 cgccgtgcag cggccattaa tgattcagcc acctggtccg ccatgccgcc gggctgccag
     7381 atataaaaca ccatctcaat attatggcgc gcgagctgga tgtcgcggat taacgcctgc
     7441 atcacatcat ctgactcggt catcagttgt agctgattcc ctttgacccc agcgatcccc
     7501 tgacgacgct cgcaaagctt gaataatggc gcagcgacac tgctattttc ttcggcgaag
     7561 atatgcttac aggctttaag gtcgttaagc cattttgcgg tggaaggcca catcgctctg
     7621 gcgcgctcag cgcggcgttt gcctaaatgg agctcgccaa cggcaagata ggcaataatt
     7681 ccgactaacg gcagaatgta aataatcaac agccaggcca tcgcggaggg aactgcgcgt
     7741 cgtttcatta gaatgcgtaa agttacgcct gcaatgagca accagtatcc cagaatggcc
     7801 aaccaactca ccaacgtata aacggttgtc atagattaga aatccttttg aaagcgcatt
     7861 gttatgagtt tacgcatatg gattcatctg gcaaataaaa acgagggaaa agcactggtc
     7921 agcgtcaggg gcgggggtat aatgaccatt ctgttgttgc atagagtagt taacatgaag
     7981 cggagtagaa cggaagtggg gcgctggcgc atgcagcgtc aggctagccg acgtaaatcg
     8041 cgttggcttg aggggcagtc gcgccgaaat atgcgtatcc acagcatcag gaagtgcatt
     8101 ctaaacaaac agcgtaactc gttattgttt gcgatctaca atatctaaat gtactgggca
     8161 ccattgcggt gcccaataag tttactcttc cgtctttaaa ctctattttt gcgccgattc
     8221 tttactgtca gctttcgttt cttgttcatc gttatcgctg aaaatgccat gaccggagtt
     8281 caacaacatt gaaacgagca tatcattatt aatctcttca ccatttaaaa ctcgcgccag
     8341 aatttcgctg ccaaacagtt gtggtgagag aatgatatcc ggatgtacca tcttaatctt
     8401 attcaggttt ttgctatcac tgacggcaag aactgttttg acatcactgc tcatatcttt
     8461 tgccgagagt acaacaaacg cgttatctgc atcgttatca ctcagcgcca gaatggcccg
     8521 gcaacgatcg attcccgctt tctttaacac tgaactgtca ttactgtcac cggggataac
     8581 atcggcgtta tcgcctaagc gttgctcaag ttgcttgata tcatcttcag gcaagttgct
     8641 gataaccgtt acgttttgtc cgcgttgatt cagttgcaga atcgtattga tggcgagaat
     8701 cgaatgtccg caaacaataa aatgatcttt acgatgcatt gtatgattgt ttccttttac
     8761 aagtttgttg aatcccccgc ggataagcgg accaaaaatt gaggtcatag atgtggcaaa
     8821 aacggtaatg ccggaaataa tgaccgaaat agtgaacaat cgtgctgatt cagaaacagg
     8881 gacaatatcg ccatagccga cggttgacat ggtttctatc gaaaaataga acgcggtcat
     8941 caaactttct attcgcggat taaaaccttc gcttaaataa agcgcaccgt aagtggagta
     9001 aaacagtaac gtcgtgaaac taataaatgc gaaaattgtc ccggctgctg cgctactgtg
     9061 ggagaagtct ttgctcagta tcagcaaaaa caccagcgta aaaatgcaaa atccaatact
     9121 aaatttcagc cagggataaa aatgcagagt gtaaattagc gctatcaaca acaaaatgat
     9181 actgattgcc catgcgagct ttgcccggaa cagtaagcca atggagttca ggacaagaaa
     9241 tacgccgagc ataaatagtg gggcgtttgc cagcaaggag agatccagct ctgaaaatgc
     9301 tttgatatgg aaaatatcaa gcaaattgac cgacatacca tagattgttt taaaaataag
     9361 caatccattt aaaaagacgg ccagcgcgag aatgtcgtgc cgtagggtca cccataactt
     9421 tgttgctgtt tgtttgaatg tagtccagtg actcactgat acctctgtaa ccacaatttt
     9481 gtttaattat ctcaacgtgc acattttctg aacatacatg cagctcgtga atgtgttaaa
     9541 caatagtaga tgactttatc cgcagtaatc aaaggttttg agtgagcaac tctgcgtcag
     9601 gaaagggcat tttcctgaat atgttcacat catataaaga aatacagata aagttattat
     9661 ctgcttgtgg tggtgaatgc actgaccgac ctataaggaa aggccaaaca agaacacggt
     9721 tgcaaaaacc gtgcccttaa atattgaatt tctattcaga acactttctt aaatggtttc
     9781 actgaaacgt gttcatagac tcctgccgct acgtacgggt cagcatcggc ccaggcctga
     9841 gctgcttcca gcgactcaaa ttcagcaata acggttgagc cagtaaatcc cgcagcccct
     9901 ggatcgttac tgtctaccgc tggcattgga ccggcggtca acaaacgacc ttcatcatgc
     9961 agtaactgta aacgtgctag atgtgccgga cgaacggaaa ggcgtttttc gaggctatca
    10021 gctttgtctt gagcgtaaat aacatacaac acaggcacaa ctccttgttc gggaaagttg
    10081 taaagtacgt tatttgaaag ggcgaagatc tgcaacggaa agatgatgtc tttgttaaga
    10141 ccatgcataa agtaaggtca attacgccaa aaatgacatt ttcactgatc ctgatcgtct
    10201 tgccttattg aatatgattg ctatttgcat ttaaaatcgg ggcctggttt ttctactgaa
    10261 atgattatga cttcaatgac ccttgattta cctcgccgct tcccctggcc gacgttactt
    10321 tcggtctgca ttcatggtgc tgttgtggcg ggtttgctct atacctcggt acatcaggtt
    10381 attgaactac ctgcgcctgc gcagccgatt tctgtcacga tggttgcgcc tgctgatctt
    10441 gaaccgccac aagccgttca accaccaccg gagccggtgg tagagccaga accggaacct
    10501 gagccgatcc ctgaaccgcc aaaagaagct ccggtggtca ttgaaaagcc gaagccgaaa
    10561 ccaaagccaa aaccgaagcc ggtgaaaaag gtggagcagc caaaacgcga tgtcaaaccc
    10621 gtagagtcgc gtccggcatc accgtttgaa aatacggcac ccgcgcgccc gacatcaagt
    10681 acggcaacgg ctgcaaccag caagccggtt accagtgtgg cttcaggacc acgcgcatta
    10741 agccgtaatc agccgcagta tccggcacga gcacaggcat tgcgcattga agggcaggtt
    10801 aaagttaaat ttgatgtcac gccagatggt cgcgtggata acgtacaaat cctctcagcc
    10861 aagcctgcga acatgtttga gcgtgaagtg aaaaatgcga tgcgcagatg gcgttatgag
    10921 ccgggtaagc caggcagtgg gattgtggtg aatatcctgt ttaaaattaa cggcactacc
    10981 gaaattcagt aagcataaag tcaaaagcct ccgaccggag gcttttgatt attactcaac
    11041 gggtaaggcg cgaggttttc cttcgggatc aaccgcgaca tacttaaata atgcttctgt
    11101 tgctttatag cgttgcccga ttggttctga cgccactttt ttcacccaca cttcaatatt
    11161 aatgctgact gatgtcgtcc ctttctggac acagcgtgca tagcagcaca ccacatcgcc
    11221 gaccgcaacc ggccgtaaga aagtcattcc ttcaacccgc acggtcacta cacgaccgtg
    11281 ggcaatttct ttcgccagaa tagcgccgcc aatatccatt tgtgacatta accaaccacc
    11341 aaagatgtca ccattggcat tggtatcggc gggcatggct aaagtacgta aaacaagatc
    11401 gccctgaggg acgttatgtg ctgtagacat ggtaaaaccg acttaaaagg aaaatcacag
    11461 gcgcgatgct actatgattt tcctgaggag aacagaggag atacagcgcc agccccgaag
    11521 gactggcagt ctggttagga tttatcttcc tgcggcatgt ggcggtagat atagataccg
    11581 cttaacaatg tgaagattaa ggtaagggcg gtcaggccaa agactttaaa gttgacccaa
    11641 atattttgcg gcagccagaa tgcgatgtag atgtttgcca ggccgcaaag gataaagaaa
    11701 acagcccagg ccagattcag cttcgaccat accgattgcg gcagggtgag ttctttaccc
    11761 agcatccgct gaattagcgg ctttttcatc acccattgac tgactaacag ggcacccgca
    11821 aacagggcat aaatgacagt aaccttccat ttaataaact catcattgtg aaagaacagc
    11881 gtcaagccac cgaagacgac caccagaaca aaagtgatca gggccatctt ctcaacctta
    11941 cgaaagcgaa cccagctata gataagcaca atcgccgtgg cgacgatcag cgccgcagta
    12001 gccgcataga tgtcataaat cttgtaaaac gcgaaaaaga caaccagcgg taaaaaatca
    12061 agaaactgct tcattttatg attccgtaat caaatgctta aggttattgg cgaatcaaca
    12121 tatacaggcg gaacagatag atgagcaata cggctgaaat caggttgctc aaggtgttcg
    12181 ccagtacggc accaatttcc ggggttaatg cggcaaaaga agaggcaaaa agcagcaaca
    12241 gtgtttttgc cagcaaccag ctcagtactg cgggtgccac cagacgcata ttcgcccatg
    12301 tcagccgcat actgctacgc atcgaggcaa aaacgcccat tttgtcctgt accagcatca
    12361 ccggtgccag agccagtaaa atggccatga taattcccgg aacgaccacc agcatgatgc
    12421 caatctgtac taaaagggta gtcagaaaaa tcagaataaa taactttggc aatatcggcg
    12481 cactggcacc aatagctcgc agcgcactga ctctctgacc cgcagacacc agctggataa
    12541 ttaatattac gcccccggcg agaatggcgt taccgattaa tcctgaaaaa gtggacgccg
    12601 ctgaagcctg cagcaaaatt tgttgctgtt ccggtgacat attctgaacc aggtcgaaca
    12661 acccactact gccgctaacg ggtacgccgt cattgagctg cgcaagctgt gcatcactgg
    12721 gtgagaaaac atgccctaac accactgtga taaacgcaca tagcaacgat accaacagaa
    12781 tggtcataaa ttgattacgg aagaaatttc cggtgtcacg gtatacggac tgcgccgtga
    12841 tagacatgca ctctccttga gttttatgca ggtgttaatt agcgggcaat tgtaccctgg
    12901 ttaagccccg ggtggcagca tcaggtcttg tatggaaaag catatctttg taaagcggag
    12961 gtaattgctg gccttcaggc gtgggtaaac cgctgcaagt ggcgagcgag ttaatatttg
    13021 cgtagcgaaa tatataaaaa ttgatttaaa tcacattaac caggattttc aatgcaactt
    13081 ctaaattaat ctagatcaat aaagggtgaa ttaccatatg taatgtgatc tgtgtaggat
    13141 catttgttac cccaatgtgg gtatattcgt cacgttttta taaccataac gacggagcgg
    13201 atatgaaaaa gttaacagtg gcggctttgg cagtaacaac tcttctctct ggcagtgcct
    13261 ttgcgcatga agcaggcgaa ttttttatgc gtgctggttc tgcaaccgta cgtccaacag
    13321 aaggtgctgg tggtacgtta ggaagtttgg gtggattcag cgtgaccaat aacactcaac
    13381 tgggcctgac gtttacttat atggcgaccg acaacattgg tgtggaatta ctggcggcat
    13441 cgccgtttcg tcataaagtt ggcacagcca caaccgggga tattgcgaca gtccatcatt
    13501 taccgccaac actgatggca caatggtatt ttggcgattc tggcagtaaa ttccgtcctt
    13561 acattggtgc gggtattaac tacaccacct tttttaataa tgacttcaat gacaccggtg
    13621 aaggtcttgg tttatctgac cttagtctga aggattcatg gggcgtggcg gggcaagtag
    13681 gcctggatta tctcatcaac cgtgactggt tgctgaatat gtcagtctgg tacatggata
    13741 tcgataccac ggctaagtac aaatctggtg ttactaccgt aaaagactcg gtacgactgg
    13801 atccgtgggt gtttatgttc tccgcaggat atcgttttta attttctctg caaaacctct
    13861 gcaaaacccc tctgcaaaac tggtcatcaa atgaccagtt ttttccattc cttaccgcgt
    13921 gcgtcgttgt aaatatcggt cattttttga ttcgaatggc ccagcaaaat tttggtatca
    13981 accccctgct ctctgaacaa tcgctctgat aaagatctct gctcatggaa agagggtggg
    14041 gtgccattag cacgccagtt gtaatccaca gaatcccggg cttttttaaa tgcaacggtt
    14101 aatgttgctg gcttaaccat cccgccgcgc ttagctgtcc ctttcgcgtg atggtggtgc
    14161 aatagccacg gactaagaac gcaatcgcgg caggatgaca ccacatcatc tagggtgaga
    14221 tttaatttat cgcaacgcag agccagaggg atggcaatcc gggttcctgt tttttgctgt
    14281 tcgacatgaa gataaccatc ccggatatcc gaaaattgca ttttgcaaat atctgaaagg
    14341 cgctggcctg tcatcagtgc cagcagcata ccgcgctgta aaaagtaacc atccttttcc
    14401 gctgcgttat aaatcatcat ccactcatca aaagtcagtc gctgtcttga tatccgcacc
    14461 tgcggttttt ttgccgattc tgcagggtta aagcctggcg ggacatcgcc cgtttgctga
    14521 gcttcccgga aaacatcgat cagtaccttc ctgaaaattt gtcccattct gttatgtcct
    14581 ctggccttgt actcttccag tactgatacc acatctttta cggttatggc atctaacggt
    14641 ctggtgccaa aacgttcatc aaataccctg agaggggccg ctttctgttt cagcgtgttg
    14701 agtttgatct cgccgttttc atatctttcc tgttgaattt ttctgtaatt attcagaaaa
    14761 atggtaacgg ttgatgaacc gccggtatca ctaataattt tctcctgcag actgagcatt
    14821 tgttccattt gctgccgggc aagacggctg ttcgcttctg ctgcaatagt ttctgccagt
    14881 ttctggtcaa tactgccgag accgtgattt ttgcctgtta tgggatgcct gtaacgccag
    14941 taaactttgt tatttctttt gtcaaaatac ggagataatc ccggaacatt ggttttatat
    15001 tttcgcgggc gcgccatctt ccagtatcct cttcaaagca gggtgatctg tggcgatcac
    15061 ctccggcttg tttaccattc cgacaaagcg agcttgcgga tccactcgcc agcgtcttcc
    15121 aacttttttg gggagaggaa atatcattcc ggctttagcg tatttactta acgtactcgg
    15181 agttgggacc ggttcactga attcctcttt tgcccactca gtgagcagaa taagtcttgc
    15241 catgagcgtc gttcgctaat catggtcgcc gccactatag ctggtgggca acgaccgggg
    15301 ttgaacatta aaaatcagcc tgattcggga tcagtttttg ccagataact gaaacgtatt
    15361 ttgcctggta acgggcgtca tcaagtgcat tatggcgctc accttcgaat ggaatagccg
    15421 ttctggcatc gaagtctatg gctttcccca gctcaacgat tgtgcgtaca tcgcgatcgt
    15481 tgtagtaacg ccatgggcag gggatcccct gccgttcgta tgaacggcgc aaaatcgtgt
    15541 tttcgaagtt ggctccattt ccccagacct gaacaaaaaa ttcaccggag ttttcgtcga
    15601 taaattcccg caattgtaac agtgcatcat ctaacgggat ttcatcggtc ataatggcag
    15661 actgcgcttc acgtgattgc ttcagccacc ttttaatgac gtcacgatca atgactccgc
    15721 cagcagtttc cagatcgata gtcttactaa attccggtcc catatctccg gtttgcggat
    15781 cgaaaaatat tgcatctatt gagataatcg gggcatcagg attttttccc atggtttcaa
    15841 ggtcaatcat cagatggtgc cacaacctgc tggtggatgt gcttttacga tgactgttca
    15901 ccttaattaa gggatttgct gtctcgccta tttcattatt gtcattggca tgttgatcgc
    15961 cttcggtgca ctccttgttc gggagtccgg cattttctat ttcctctgga tctttttccc
    16021 gggcttcatc ctggctttct tcgttgaatg tctcctggta ggttgcgtca cccatcactg
    16081 cgccacaatc agggcagtta ccaccaccag tctgattgca tgcggtacaa actttttcct
    16141 cttcctgttg cacgactggt tcaggttgtt tcgtttctgg ctcgttttgt aacgcatttt
    16201 ggctgttttg ttccgctttt tggtcgtact gttccgtttc tgactggttc tggtgcacag
    16261 aatcgcgggt ttcaatcccc ttcacccatt tcggatcatt cgggtcgcta atcccttcaa
    16321 caaattcacc acgtgatgca gcgagcaact tattagcgtc aggctggctg atactggctg
    16381 cctgcataat tttgtttact tcgtcagcgg taacttttac caggtctggt tgtgcggtcg
    16441 tgtcagatgc atcagtattt tgttgtgaac ctgtgtatgt gccgtttttg cgggcaaaat
    16501 attcttcttt cgtgatttca gttgccccgg cagccagcgc cttatccaga ccagaaagtt
    16561 tgtttgcgcg accgtatttt tcgccatcct tgtcggtgaa gaggaagtag aacggcccct
    16621 cacgctctac agatggttcg acttccactt tgcattcggt tttttcgttg tccggaattg
    16681 ccgtttccac tgcatcagtt tctggtactg gcgacgagag agtatcagtt gcgctctgat
    16741 ttcttccttc atcttcaaac acgccctttg tagtcaggta ttcagtaatg tatttgttca
    16801 gtgccacagg gtctttgtga atgtcgatcg gacgttcacg gacaaggcca aaaatagtct
    16861 ggcggtcgta gcgaagggca tcaggctgtt tgcgcattga tgccgagata cgcttccagt
    16921 cttcgcggtc gttgtcgata acttcatttt ttgcccagcg atggatgctg ccgtcaatgt
    16981 ttccggcatc cacatcacca ggccagagag cgtaggccag ttcgtcatcc agtgttttcc
    17041 atgtctgctt gtattcgcga tgaatggcag caatgaccgg gctgattttt cctgttgaat
    17101 tttcagtgta ctgttgattg gctctggcgc gggcgagatc aacaacagac gtgtattttc
    17161 cggtttcctt gcgttcacct tcacgacgtt ttttccagat gcgcatctct gcctgaattt
    17221 cgggccattt ggcaccaggc ttacatttat gcttaaccca cccgatggca tgcagcttaa
    17281 gctccggata catggcgtta acttctggca ttttcatcaa cgcttcaacg atatgtccgt
    17341 cgaatgttgc catgtcttcc tgcaacaatt cctgtgcgct aataaccata tcaacggtga
    17401 tgttttcaca tgtgtcgaac ttaaccatga cagcgttctg tacttcaggg gccagcttgt
    17461 caaaagtgac gttcatcgga tcggattcag tctcaaccgg gacaaaggaa gcagactcct
    17521 catcccagcg gttttcctgc atatattcag catcccagga atcgagggca gggcggggta
    17581 taccgggttt atcctcgcag acaagaaatt tataagcgca gtcctgagca gccggataat
    17641 gttccaggaa ttgccagtga aattttgcgc gggcgcgacg ttcatcaccg gcttcaatgg
    17701 cagtggctac agcgacggca ccttcttcct ttattgcctg ttcgtccgga atggcggcgc
    17761 aaataaagac tttactcatt ttgttttacc tcattacaga tttaagggtg aacaaatccc
    17821 tgccattgct ggcatataag aatgaaaccg gatatttatt acggaactgt tttaaagacc
    17881 tgccgggatt tcgttattat cctggtgaat aactttatcg accgggtaac agttaccggg
    17941 aattttctgt tcggttgctg cagtcacaca ctcctgcatt gtcctgtgaa cactgactgc
    18001 aatatcaact ggctctccgg aaacaagaaa aactgtcaga acaagtgcaa atgctgtatt
    18061 cattgccagc atcctttttg tatcggacgt aaacgggcca gcattgaaag aatgcatatt
    18121 ttatttaata gctcccgttc gtgttttctc ttgttaatgg catcttcagt aaatacaggg
    18181 ttactgatat tgacaccaat ttcaaaacaa ccttcagacg tattaacgtt tggtaataac
    18241 gtttccatta tcgcgtcctc aacaatgaat tttgtgatgc ggtgcctggt gcctccaggt
    18301 gacgttaacc agttaacaat taacgccgga tacagagaat ccacccataa cactgttttt
    18361 ggttttaact gttccgcgtg cgctcagccg cattcaccac atcacaaaat tcactttaaa
    18421 aagggcggca gagcagtcac ggagtaaaac tgataccgcc aaacgtcacc agaaaattga
    18481 taacagaggg cgttgcagcg gagttgtcac ttaagcgtat ggtcaacctg acaacccggt
    18541 gtcctcaacg gaggaaggaa taaccccgcc atacttaccg ccgcgccatt tcgcggagtg
    18601 ccacaaccgg aagcgcacgg tcgaactaaa tttaacgaca ccgtacagag agaccaattt
    18661 cgccgtgcgc tttcgcttta tgccctgact tttcagggac atatcctttc agtaaactgt
    18721 cagtgccgga tgttcacccg tgtccggcgc acgcactcca cctgacccgt ggagaactcc
    18781 ttaattacca accttagctt cgttggttag ctattaacgc gggtatgtaa ccattctggc
    18841 aatgcttaat gccgctgctt tttccagcct ggtgatatcc tgctccagag cggacagatt
    18901 ttcagcctgc ttagtcctgg cttcattggc ccatttcaga tcctgcgctg cattaatttt
    18961 ctggcgcatc cactcataaa gttcatcatc ggtatagtct ggcgcgatga tgacgggttc
    19021 tcgtttctgc atactgattc ctcgcggtgc tgcttcgctt atcagccgtt agattttgcc
    19081 gagctggaaa gcgcctgttt aaactcactg aagctgagag cttcttcgcc ttcggcaagg
    19141 ccttcgaagt attcttcgta agccttttcc atgattgtgt cgaaatccat atcactcacc
    19201 tgagtttctt tccagccagc gacgggcacc attttcggtt ttaaacgttt tgcttttggt
    19261 atacgtcatt gcggtgaacg tgccgtcctg gttggggaac acgccacata ccagagattc
    19321 gctgttgcca agatcgatag tatccatgct gacctcattt ccccttaacg ccgggggagc
    19381 ggaacaaaaa cctgctgcat agttattaaa gttgaaccct gccgtcatgt tcttacgcct
    19441 cgggctggct acttaccccc tgaccactgc ttggtaactc gaagtattgc ccggcgttct
    19501 gtggggcggg gtgggttggt atgttgttaa ggtaacaaga gttacctttc gagtcaatac
    19561 aatgttgcaa aaggtacatt tgagggcata aaaaacccgc aatgaatgcg ggttctgact
    19621 cagtctaagt attgatgtat ttgtgaaact ttacctttaa tggtgtaacc accattcagt
    19681 tcgatgggtt tgtaaagcgg attcagtgac aacagataga tgtttggtcc gtcaatcgca
    19741 acttttttta gtgttacgtt tggcgttcct tccaattgga ttaagattat ttttcccacc
    19801 agttctctaa tgttacttga gcatggtgtg atcagcacgg tagatccgtc ggggatggtt
    19861 gggaggccgt tagagtttgt catcgcatct ccctcaacat gcaataaaaa agagttttca
    19921 gcggtttttg tcatgacatc aacccagttc ttaataccag gaatcttggt tactggacaa
    19981 ctcatatccc aataaccagc ctgttcccac gttaaaacgg gcaaccgggc gatgttgtca
    20041 ctaatgtaag ggtactgatt cagacgcaga tcatcggttt tatcgtgacc gtcctttcca
    20101 taaagaatcc attcaggaga tttggaaagc aattttgaca gtagatacaa attctcaccg
    20161 tcaggttttg aagagccatt ttcccatttt gttacggata cacgagatat gccgattgct
    20221 ttcgcaacct gctgttgggt taatccaacg tcttttcgac gattccgaat acgttcgctg
    20281 atagtgtttt tcatgtaacc aatgttacta ccaagtgatg ttgctatggt tgacattgtt
    20341 atgtaactat tgttaccctt ctgctcgaaa taacaggaga gttttatgtt caaagatgat
    20401 gttctgcgct atttcaaaaa aaagcgacta gtagctgagg ctcttggaat ttcacatgtg
    20461 gctgttgtgc ggtggaaagc agttattccc aaacttcgcg caatggaact ggatgaaatt
    20521 actaacggtg aattgaaata caacccagaa ctttacaaga agcaggatag cacctcgaac
    20581 gaaggaaaga atgattcatg aaaatcaagc atgaacacat ccgcatggcg atgaatgcct
    20641 gggcgcatcc ggacggcgaa aaagtgccgg ctgcgaaaat taccaaagcg tatttcgagc
    20701 tgggaatgac gttcccggaa ctgtatgacg acagccatcc ggaagccctg gcccgtaata
    20761 cccagaaaat tttccgttgg ctggataaag acacccctga tgctgttgaa aaaatgcagg
    20821 ctctgttacc ggcgatcgaa aaggcgatgc cgcctttgct ggtggcccgt atgcgcagcc
    20881 acagttctga atattaccgt gagatcgtcg aacggcggga tcggctggtg aaggatgtcg
    20941 atgattttgt tgcgtcagcg gttgttttgt atgaccagat gaatcgcggc ggcccggcag
    21001 ggaatgctgt ggtgatgcac taaaagcacg gtgttcgggg gttttatgag cagcaagctt
    21061 catggtcttg tctgggaagg gtgcgccttc accggcatga tcttatccag ggtggcggtt
    21121 atggcccgtc ttgcagacta cagcaatgac gagggcgtgt catggcctgc cattgaaact
    21181 atccggcgtc agatcggtgc aagaagtgaa tccacagtga aatcggctat tgcagaactg
    21241 gcgaaagagg gctggctgac gaaggaagag cgtaaggtcg gtgggcgtaa tgtaagcaat
    21301 atctatcggc ttaatgtgga aaaactcgaa gcagctgcgg cggcggcgcg tgagtcatat
    21361 aaaccgaaaa gaaaaattag cccggcaaaa aatgacccgt taacagttga cccgtcaaat
    21421 attgacccct caacggttga cccgtcaaat tttgatggat caactgttga taaaaaactg
    21481 ccgattaggg ggccgatgat tgaccccgat ccgtcagtat taaaacctga tccgtcagat
    21541 aaaagatctt cttgtccgga cgcttcgcaa ccggacccgc agacggctga acaggatttt
    21601 ttaacccgac accctgacgc ggttgtgttc agtgcgaaaa aacgccagtg gggaagtcag
    21661 gaagatttgg tgtgcgcaca gtggatctgg ggacgaatcg tgagtcttta cgagcaggcg
    21721 gccagctatg atggcgagat cactagaccg aaagaaccca actggacagc atgggccaat
    21781 gacgttcgca caatgcggat gctggatggc agaactcaca gacaaatttg tgaaatgttt
    21841 gggcgtctcc agcgggattc gttctgggta aaaaacatca tgagtccggc aaaactccgg
    21901 gaaaaatggg atgaactggt tatccgcctg gggcgttcgc ctgcgcagcg ttgcgtgaat
    21961 cacatttctg aaccggacac tgaaataccg ccgggattca gggggtgacg tgtcatgaaa
    22021 aacattgcgg cagttggggt tcttgaacgt attcgcagac ttgcaccaca ggggtcggtt
    22081 ccaccgtacc ggacggtgga ggagtggcgg gaatggcaac ttgctgaagg acgaaaacgc
    22141 agcgaggaga ttaaccgcca gaatcgccag ttgcgggtgg aaaaaatcct gaatcgttcg
    22201 ggcatccagc ctctgcacag caaatgctcg tttgcaaatt atcaggtgca gaacgacggg
    22261 caaaaatacg cgctgagcca ggccaaatcc atagctgacg aactgatgac cgggtgcacg
    22321 aattttgtgt tcagcggtaa aaccggcacc gggaaaaatc accttgcagc ggcgatgggc
    22381 aaccggctga tggtgaaggg gcgcagcgtg attatcgtca ccgtgtctga cgtcatgagc
    22441 gtgttgcatg acagctacga caacggcaaa tccggggaaa aatttttaca ggagctttgc
    22501 ggggttgatt tgctggtcct ggatgaaata ggcgttcagc gggagacgaa aaacgagcag
    22561 gtggtattgc accagataat tgatcgccgg acagcatcac tgtgcagtgt cgggatgtta
    22621 acaaacctga atcatgccgc aatgagtacg cttcttggtg agaggattat ggaccgcatg
    22681 accatgaacg gtggtcgatg ggtgacgttt aactgggata gctggcgtcc aaatgtcagc
    22741 aatatgaggg ttgtgaagta attttgtccg gaggaaattt taatggaaac cgtatctgac
    22801 gcactgaaag cactgaaaaa agcctcttca catgtggtgg cagctcgcct tggaatcagt
    22861 cgtgaagagg ctgtcaacga gctgtgggaa ctcaaaagaa aaggcgtcgt tgataaaact
    22921 ggtcacacct ggtttctggc tggcgaaggt gaatcccggg taaccgaaga gcggccagta
    22981 aaatctgaag cacaggatat gctgaccggg gaggtcgaac aaaaagttac cgcagacatg
    23041 atgattgagt ttatcggtca ggatggggct aaaacgtgtg aggaactggc gggtaagttc
    23101 ggtgtcagta ctcgcaaggt tgcttccacg ctggcggtgg taaccgcaac ggggcggctg
    23161 gcacgcgtta atcagaacgg taaatttcgt tactgcatgc cgggcgataa tttaccagca
    23221 gagccgaaag ccgcgctggt aacggaaagt gatggtaagg cctttcctca gccagcaggt
    23281 gctgcgttac cagtccggga agccgcaaca caggaagaaa ttaaaacaga aactgtggcg
    23341 gacattgtgc agccgttgcc atcgtttacc gaaacgcaag cagatgagct gatttttccg
    23401 tcccttcgca gggcaaacct ggcgctgcgc agggcgaaaa gtgatgttca gaagtgggag
    23461 cgagtctgcg ccgcgctgcg ggagctgaac aagcaccggg atattgttcg acagattact
    23521 gattcttccc gccgtgttgt atcggaaaag tgattgccgg aggcgcttat ggcaaaagta
    23581 tttacacaag aagagcggga aaaaattaaa gggcaggttg ttgaacttgt acgtctgagc
    23641 ggtcgcgaga cgttgcggca actggaagcc aagacaggtg cgacaagata tctgatgagt
    23701 gttctcgcca gagagctggt tgccagtggc gatgtataca actctggtta cgggttattc
    23761 ccgtctgaac aggcgcgtaa ggactggcaa aatgctcgca aaaaactctc aagggcaaag
    23821 gtgaagaaac ctgcagtggt tgatccggac cttatctggt cgttaccaga cggcgaaata
    23881 cgccgctacg acaggcgcct gaatataatc tgtcgcgagt gccggaagag cgaagctatg
    23941 cagcgtgtac tggcatttta tcaaggaaat gttaggtatt ttagacgtta ctagattaaa
    24001 gagcattagt tcagatgtga attgacattt tcatggcgca gggtagagcc agcgtggttg
    24061 tccgctttgc gtcaaaacca gatattacca gatttagaca tatattcccg atagccctgc
    24121 tctgatgcta cactctgtgc tattttcatg accccaataa aaatatttat gactattgct
    24181 gatttcaaac ggcctaaatt ggagctccca aacggggcaa acaaactact actgcactct
    24241 tgctgtgctc catgttccgg tgaagtgatg gaggcgcttc aggcctcggg aatcgactac
    24301 accatctttt tctacaaccc gaacattcat cctcagaaag agtatttaat tcgtaaggat
    24361 gaaaatattc gctttgctga acaacacggc gtgccgttta tcgatgctga ttacgacacc
    24421 gacaactggt ttgaacgtgc caaaggaatg gaatgggagc ctgagagggg gatccgttgt
    24481 accatgtgtt ttgacatgcg ttttgagcgg acagcgttgt acgctgctga aaatggtttc
    24541 agtgtgatca gcagttcact gggcatttca cgctggaaaa atatgcagca ggttaacgag
    24601 tgtgggcggc gagctgttgc gcattatccg ggtatggtgt actgggatta taactggcgc
    24661 aagcagggcg gctcgtcccg tatgattgaa atcagcaagc gcgaaaaatt ctatcagcag
    24721 gaatattgtg gctgtgtgta ttctctgcgc gataccaatc tacaccgcaa atctcaggga
    24781 cgccctctta tcaaaattgg ccaactccac tacggaaaag aagagaagga gtgattttat
    24841 ggatcacctt tctgattgat ttcatattgg cgaggtgacg tgagttaagt agaatggctg
    24901 cgggtgcttg aggctatctg tctcaggcat gaacactgaa aggcagatag agaaaagccc
    24961 cagttaacat ttcgcgtcct gcaagacgct taacattaat ctgaggccca atctatgtct
    25021 cacaaatgta ggttagcctc ttacgtgccg aaaggcaagg ggaagcaggc tatgaagcag
    25081 caaaaggcga tgttaatcgc cctgatcgtc atctgtttaa ccgtcatagt gacggcactg
    25141 gtaacgagga aagacctctg cgaggtacga ctccgaaccg gccagacgga ggtcgctgtc
    25201 ttcacagctt acgaacctga ggagtaagag acccggcggg ggagaaatcc ctcgccacct
    25261 ctgatgtgtc aggcatcctc aacgcacccg cacttaaccc ggttcggcgg gttttgtttt
    25321 tttctggcat tctggtttac aattcgcacg tcagcctgaa cacctgacac ctgctgcgcc
    25381 agcagagaaa acagatggcg cacaaaacca aatttcacaa ttctgatacc gaccttgcca
    25441 tccggcatgg gcggcgttca cacgcattta aaaccgactg gtaccaacac ccaccatgta
    25501 ctgaagaaca ggccgaatgg ctaattcata actaccgcag acgcggatac gagattaaga
    25561 aagccctcag cctcgattat cgtcactgga taatctccgt caggcttcct tactctgaac
    25621 gcccaccgcg tccgtcccgc acattccagc aacgcatctg gaggtaacgt gcgggtatta
    25681 cttcgacctg ttctggtacc ggaactcggg ctggtgatcg ttaagccggg ccgtgaatcc
    25741 atgccggtat tccacaatac ccgggtactg gtggagccgg aaccgaaaag catgcgtaat
    25801 ctgccgtccg gggtcgttcc tgccgttcgc cagccgctgg cggaggataa atcattactg
    25861 ccatttttca gcgacgaacg agtgattcgt gctgctggtg gcgctggcgc attgtctgac
    25921 tggttactgc gccatgttaa atcctgccag tggccacacg gcgattatca ccacagtgaa
    25981 accgtcattc accgttatgg taccggcgca atggtgttgt gctggcactg cgacaaccag
    26041 ctgcgcgacc agacctccga atcactcggg caacttgctc accaaaacct gtttgcatgg
    26101 atgattgacg tcatacgcca tgcaatgaat ggctcgcagg aacgggaatt atcgctggct
    26161 gaattatcct ggtgggcggt ccgcaatcag gtggcggacg cgctaccgga agcggtatta
    26221 cgtcgttcgc tggggttgcg tgcggaaaaa atccgctcaa tgtaccgtga aagcgacatc
    26281 gtaccgggag agcagaccgc caccagcata ctgaagcagc gcacaaaaaa tcttgcgccg
    26341 ctgcctcacg cccaccagca aaacccgcca caggaaaaga cggtggtcag cattgccgtt
    26401 gatccggagt caccggctca gtatctccag cgccagaaat cacaacggga agagatgcct
    26461 gtatacacgc gttgggtaaa aacgcagaaa tgcatgacgt gtggcaatca ggcagatgat
    26521 ccgcatcaca tcattggtca tggactggga gggatgggaa caaaggctga tgatttgttt
    26581 gttattccgc tgtgccgtaa atgccatagc gaactacacg ccggggtaaa agattttgaa
    26641 gaaaaacacg gcagccagct gttgttgctg attcgttttt taatgcacgc gagaaattcg
    26701 ggtgttctga agtggaaagc ataaatgact gaacgcatag aatttgtttt gccttacccg
    26761 ccaacggtga acacttactg gcgtcgtcgt ggcagcacat attttgtatc aaaagccggg
    26821 gagcgttatc gccgggcagt ggcgcttatt gttcgccagc agcggctgaa attaagcctg
    26881 tccggaaggt tggcaataaa aattattgca gaaccaccgg ataagcgccg ccgtgatctg
    26941 gacaatattc tgaaagcgcc gctggatgcg ctgacgcatg cggggttgct aatggacgat
    27001 gagcagtttg atgaaatcaa tatcgttcgt ggtcagccag tatctggtgg acgtctgggg
    27061 gtgaagattt accccataat gcatgaagag caggtcaaaa aatgaaactg gaagatttac
    27121 cgaaatacta ctccccaaaa tcccccggcc tgactgatgc atcggcctca acgtcgaaag
    27181 atgcgctgag tatcactgat gtgatggccg cgcagggcat gacacagaat cgggctgaga
    27241 tggggttttc tgcgttcctg gggaaaatgg gcatcagtat gaatgacagg gcgcgggcaa
    27301 cagaattact ggcagattat gcactcagtc ggtgcgatcg tgtggcggcg ttgagaaaac
    27361 ttccggcaga aataaaaccg gtagtgatgc gcattatggc ttcgtacgct tttgaggatt
    27421 atgcccgcag cgcagcgagt aaaaagcagt gcccctgttg ccgaggggaa aaatttattg
    27481 aaggcgaagt ttttacaaac aaggttcagt atccggatgg caagccgcca gtatgggcaa
    27541 agtgtacgaa aggtgtgtat ccgtcttact gggaagaatg gaaaaaaatt cgggaggtgg
    27601 tgaaagtttc ttgtcctgaa tgtaaaggga agggggagat ttccactgcc tgtaaagact
    27661 gccgtgggcg tggtgtcgcc attcatcgtg aagagtcggt aaaacgtggt atgcctgtta
    27721 tcagagactg ccagcgttgt ggtggtcgtg gctgtgaaag actaccatca acggaggcat
    27781 ttaatgccat atgcaaagtg acgagtgcta tcacgcttga tacgtggaaa aaatcagtga
    27841 aacgctttta cgatacgttg gtggttcggt ttgacattga agaggcatgg gcggagcggc
    27901 agttaaagag ggtaacgcga tagtgttgtt gatttttccc gaatctgtgg taaatttgct
    27961 ctaacgatgg gcgttttatg cctgacgtta gaagattttt tacaccccgc cgcctggcgg
    28021 gttttttatg actgaaatcg cgtcagtaca gtaaacgcgc tggtggcggt gaatacctgt
    28081 ctttcagctt gctggctttt tcgacaagag ttattggtgt gtcacgttaa ccggaaaagg
    28141 gaaaaagaca tgctgaaaca gcaggatatg acagaaaccg ccagagtggt gtttaatgaa
    28201 ttaagcgtca ccgaaccggc gacagccggg gagattgcgc agaatactta cctttcacgc
    28261 gaacgctgcc agttaatact gacccagctg gttatggcgg gtctggcaga ctatcagttc
    28321 ggttgttaca gacgccttcc gcagtgaagg cttttttatt tgtggtaaat gggcggctgg
    28381 tgggtgttag gggcacccac cagccatctg ctcatgcgtt ggggtcacaa gcaaacctca
    28441 ggcccatctg ctttgcgcaa aagcggtatg agcctatcag agaagtgctt attgatctat
    28501 ggctaatact gtaaaaatat ccagttgtga gttaatcaac gccgactgcc tggaatttat
    28561 ccggtcgtta cccgaaaatt ctgttgacct gatagtcacg gacccgccgt actttaaagt
    28621 gaagcctgag ggctgggata accagtggaa gggcgacgat gattacctga agtggctgga
    28681 ccagtgtctg gcgcagttct ggcgggtgct gaaacctgcc ggaagtcttt acctgttctg
    28741 tggtcatcgc ctggcatctg atatcgaaat catgatgcgt gaacgcttca gtgtgctgaa
    28801 ccatattatc tgggcgaagc cgtccggacg ctggaacgga tgcaacaagg aaagcctgcg
    28861 ggcgtatttc cccgccacag agcgcattct gttcgcggaa cattatcagg ggccgtatcg
    28921 tccgaaagat gccgggtatg cggcgaaggg cagtgcactg aaacagcatg tgatggcccc
    28981 gctgatttct tactttcgtg atgcgcgcgc ggccctgggg ataacggcaa aacagattgc
    29041 agatgccaca ggaaagaaaa acatggtgtc gcactggttc agtgccagtc agtggcagct
    29101 accgaacgaa agcgattatc tgaaattaca gtcgctgttt gcccgggtgg cagaagagaa
    29161 acatcagcgc ggtgaactgg aaaagcccca ccaccagctg gtggatacgt atacgtcact
    29221 gaaccggcag tatgtggagc tgcagagtga atataagcat ctgcggcggt attttggtgt
    29281 gacggcgcag gtgccgtaca cggatgtgtg gacacataaa ccggtgcagt tctatcccgg
    29341 gaaacatccg tgcgaaaaac cggcagaaat gctgcagcag ataatcagcg caagcagtcg
    29401 tccgggtgac ctggttgcag atttttttat gggctcaggt tcaacggtaa aagcggcact
    29461 ggcgctcggg cgtcgtgcga ttggcgttga gctggagacc ggacgttttg agcagacagt
    29521 cagggaagtt cagggtttaa tcgtttgaaa cggatgagat tgcagaatta attacgcacc
    29581 attattattc tgctcccggc cctttagctc agtggtgaga gcgagcgact cataatcgcc
    29641 aggtcgctgg ttcaaatcca gcaagggcca ccatcacaaa ccgccattag cttatcagga
    29701 agagcagacg acacgataac agggttgttg gtgcgggggc gggtccccga tggcggtcca
    29761 ttatcggtat tcagcgttgt tagctcagcc ggacagagca attgccttct aagcaatcgg
    29821 tcactggttc gaatccagta cagcgcgcca tattcattct tccagattcc ttccggcaga
    29881 gccttatact gaaatatacc tggctcagga tattgttgaa aatattatat gtttgtcaaa
    29941 aataaaagtt ctgttaagtg ttgattgagt gtttgttata cggtctaatg gttttttcag
    30001 cattaaatat ttatcattca tatggtgtgg gtagagtgaa tattgatgag gcgtcggggt
    30061 gtttcatcct taggcagcgt attgatatag tcaatgcagc acgagcaaag gccttcagcc
    30121 gtttgacagt tttgttctgt actcctgatc gtctttcggg aagagacgtt attattctga
    30181 atagtgatgc tatacagagg gtttgcgatg agttcatggt ggctaattca gaattattta
    30241 ctcttgttca ggagtacaac agaatagcca ggacctgtgg tatggatgaa cttcggatta
    30301 ctcatctggg gtagatacat atctggatta tcaccggtta cggtaaaaag tgattgctta
    30361 ctgtttttgt gaatggcatt gctgcagccg gataatgtca gtgctggctg acggtgtgct
    30421 ggtggcgggt gtggtggttg ttgctttccc gttgctgaaa aagaaaacgc cagactgtta
    30481 gccgggtatc agttagcggg agaaattttt aaatacttca caattcaggc ggttgattgt
    30541 tgtctggttt gcggggagtt tgttaaaaga aactggcatg gtgaatcccc ctgtgcggag
    30601 gggcaatcag cgagtaggta tatgggataa tcgcggattc aggtgctggt actgaattca
    30661 ccgggaggca cccggcacca tgcaatggca catagcgcca ctctccagcc cctctccgga
    30721 ggggctgttt atattgattt tgtcagatgt gagtaaactc cttatggact ttgttgtttt
    30781 agtccataag gacatatttg cagagtgcaa cggttattaa agcattcatt caatacgtta
    30841 tctgtatttg tagggcattc ctggctgttt ttgattaaat tccagaatgt tttattgaat
    30901 ggtactacgt tgtaaatggt tacaggtagc actt
//
LOCUS       DACADF010000046        30458 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.046, whole genome shotgun sequence.
ACCESSION   DACADF010000046 DACADF010000000
VERSION     DACADF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.046"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..1847)
                     /gene="gss"
                     /locus_tag="ISB30_001971"
     CDS             complement(<1..1847)
                     /gene="gss"
                     /locus_tag="ISB30_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAP1989603.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPQGQQ
                     WTRELEMVVENGGYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDQTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIV"
     gene            2052..2918
                     /gene="yghU"
                     /locus_tag="ISB30_001972"
     CDS             2052..2918
                     /gene="yghU"
                     /locus_tag="ISB30_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAP1989604.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFASINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(2988..3236)
                     /gene="hybG"
                     /locus_tag="ISB30_001973"
     CDS             complement(2988..3236)
                     /gene="hybG"
                     /locus_tag="ISB30_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAP1989605.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3249..3590)
                     /gene="hypA"
                     /locus_tag="ISB30_001974"
     CDS             complement(3249..3590)
                     /gene="hypA"
                     /locus_tag="ISB30_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAP1989606.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3583..4071)
                     /gene="hybE"
                     /locus_tag="ISB30_001975"
     CDS             complement(3583..4071)
                     /gene="hybE"
                     /locus_tag="ISB30_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAP1989607.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDSV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVANPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4064..4558)
                     /locus_tag="ISB30_001976"
     CDS             complement(4064..4558)
                     /locus_tag="ISB30_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAP1989608.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(4558..6261)
                     /gene="hybC"
                     /locus_tag="ISB30_001977"
     CDS             complement(4558..6261)
                     /gene="hybC"
                     /locus_tag="ISB30_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAP1989609.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6258..7436)
                     /gene="hybB"
                     /locus_tag="ISB30_001978"
     CDS             complement(6258..7436)
                     /gene="hybB"
                     /locus_tag="ISB30_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAP1989610.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7426..8412)
                     /gene="hybA"
                     /locus_tag="ISB30_001979"
     CDS             complement(7426..8412)
                     /gene="hybA"
                     /locus_tag="ISB30_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAP1989611.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINSPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8415..9533)
                     /gene="hybO"
                     /locus_tag="ISB30_001980"
     CDS             complement(8415..9533)
                     /gene="hybO"
                     /locus_tag="ISB30_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAP1989612.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMVESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9722..10009)
                     /locus_tag="ISB30_001981"
     CDS             complement(9722..10009)
                     /locus_tag="ISB30_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAP1989613.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10128..11015)
                     /locus_tag="ISB30_001982"
     CDS             complement(10128..11015)
                     /locus_tag="ISB30_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAP1989614.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKSNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGISGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDSRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11172..12212
                     /gene="gpr"
                     /locus_tag="ISB30_001983"
     CDS             11172..12212
                     /gene="gpr"
                     /locus_tag="ISB30_001983"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP1989615.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12252..12746)
                     /locus_tag="ISB30_001984"
     CDS             complement(12252..12746)
                     /locus_tag="ISB30_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAP1989616.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            12937..13821
                     /gene="yghA"
                     /locus_tag="ISB30_001985"
     CDS             12937..13821
                     /gene="yghA"
                     /locus_tag="ISB30_001985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAP1989617.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13860..14285)
                     /gene="exbD"
                     /locus_tag="ISB30_001986"
     CDS             complement(13860..14285)
                     /gene="exbD"
                     /locus_tag="ISB30_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAP1989618.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14292..15026)
                     /locus_tag="ISB30_001987"
     CDS             complement(14292..15026)
                     /locus_tag="ISB30_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAP1989619.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15278..16465
                     /gene="metC"
                     /locus_tag="ISB30_001988"
     CDS             15278..16465
                     /gene="metC"
                     /locus_tag="ISB30_001988"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAP1989620.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGKIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16605..17264
                     /locus_tag="ISB30_001989"
     CDS             16605..17264
                     /locus_tag="ISB30_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP1989621.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17304..18203)
                     /locus_tag="ISB30_001990"
     CDS             complement(17304..18203)
                     /locus_tag="ISB30_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1989622.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18397..19560
                     /gene="yqhD"
                     /locus_tag="ISB30_001991"
     CDS             18397..19560
                     /gene="yqhD"
                     /locus_tag="ISB30_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAP1989623.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPSRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19665..20492
                     /gene="dkgA"
                     /locus_tag="ISB30_001992"
     CDS             19665..20492
                     /gene="dkgA"
                     /locus_tag="ISB30_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAP1989624.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20692..21618
                     /locus_tag="ISB30_001993"
     CDS             20692..21618
                     /locus_tag="ISB30_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAP1989625.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALILNDNLTLPGNIGWLDYDPVCDCQDFGNLVLESVAITQTD
                     ADHADAAVRFRIFQDDKEKTSQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     TIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21669..21926
                     /gene="yqhH"
                     /locus_tag="ISB30_001994"
     CDS             21669..21926
                     /gene="yqhH"
                     /locus_tag="ISB30_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAP1989626.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(21968..24187)
                     /locus_tag="ISB30_001995"
     CDS             complement(21968..24187)
                     /locus_tag="ISB30_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAP1989627.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKS
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICSHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            24439..25188
                     /locus_tag="ISB30_001996"
     CDS             24439..25188
                     /locus_tag="ISB30_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP1989628.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIISLQENAIAAESDRFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
     gene            25511..26983
                     /locus_tag="ISB30_001997"
     CDS             25511..26983
                     /locus_tag="ISB30_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP1989629.1"
                     /translation="MSGNESIAAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVAGGIH
                     TPRDGIAAAIHKLAEPQVKIVSLTITEKGYCLDPQTRSLDLTNGLIQHDLQNPDAPLS
                     AIGVIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKA
                     GAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITHQLMINE
                     QARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIDVVEKSQSVADPMNDQFQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
     gene            26980..27996
                     /locus_tag="ISB30_001998"
     CDS             26980..27996
                     /locus_tag="ISB30_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP1989630.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVGICGTDIHAF
                     AGRQPFFSYPRVLGHEICAEAVSRGSQCQTAQSGQRYSVIPCIPCGECAACREKKTNC
                     CERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
                     EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINVFDPTQEGFIA
                     ALSEVFGGELACVVLDATGNKASMSHDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKE
                     TTLLSSRNATREDFALVIELMRSNKIHENLMKNQAFNFFSVGEDYQRNVVENKNMVKG
                     VITF"
     gene            28007..29017
                     /locus_tag="ISB30_001999"
     CDS             28007..29017
                     /locus_tag="ISB30_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HAP1989631.1"
                     /translation="MTTVYVSEENLKSLVHHKLHTAGLDADTAQQVTDVLIHADITGV
                     HSHGVMRVEHYCTRLAAGGLNKAPQFSIEQISPSVAILDSDDGMGHSALISATEHTIK
                     LALEEGLGFVSVKNTSHCGALSYFAEMVTNKGLVAIVMTQTDTCVAPHGGAERFLGTN
                     PIAFGFPVENSHPMIVDMATSATAFGKILHAKETGKHIGEGLAIDKDGYGTTDPHKIE
                     NLLPFGQHKGSGIALAIDALTGMLMNANFGNHIVRMYGDYDKMRKLASLVIAIDPKKL
                     GNPVFAKTMAQMVTELHAVKPAPGIEKVLAPNDPQMHYKEKCQQEGIPVPAGIFHYLA
                     EN"
     gene            29090..30073
                     /locus_tag="ISB30_002000"
     CDS             29090..30073
                     /locus_tag="ISB30_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP1989632.1"
                     /translation="MKALRPLTASLMLLTSCLLISNTTLAKTTLKLSHNQDKSHAVHK
                     ALSYLADKTKEYSNGELVIRIYPNATLGNERESLELMNSGALQMVKVNAASLESFAPD
                     YSLFSLPFLFRDRDHYYRVLQSDLGKKILKSSESKGFVGITYYDGGARSFYSNKPITK
                     PEDLAGMKIRVQQSPSAIAMMKALGGVATPMAQGELYTALQQGVVDGGENNTVVYSDM
                     RHAEVAKVYSRDEHTMVPDVLIISTDVLNKLGDKERTALLKAADESMMQMKDVIWPAA
                     EKEAYDKMKGMNATVVDVDKSAFKERVKPLYDEFKAKDAQSAKNLELVESM"
     gene            30115..>30458
                     /locus_tag="ISB30_002001"
     CDS             30115..>30458
                     /locus_tag="ISB30_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP1989633.1"
                     /translation="MRTLTHILNKILAGCCCIILAIMVFCVTWQVIARFIFNAPSTVL
                     DEFTQILFMWMILLGGVYTAGLKKHLAIDLLAQKLPAASVLTLDSFIQIIITVFAVIF
                     MIYGGNIVVEKA"
BASE COUNT         7748 a         7851 c         7538 g         7321 t
ORIGIN      
        1 acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc accgcgcaaa
       61 cacgtcccac cgtagttgcc gccaacggtg aaggtacata cctgaatgta tttaccgtcc
      121 actttcggca aacaccacag ttgctggtaa atatttttct gttcggcaaa tttaccactg
      181 gtctgatcaa gcacctcttc atgatggctg accaggtcaa tgttgctacc acagcgcccg
      241 gcaatcggtt taacggcata accggttttc accagctcat cattcacggt gaagtcagta
      301 tccaacagat aacgatggtg cgggaacaga gaccagagga tcggcagaat cgctttgtta
      361 ccggggatca ccgtccatag cggctcaaaa accaacactt ccgggcgcag caatacgtcg
      421 ataagacgca cttcattttg tggatgcccg gtacggatcg gcactgcggc aaattcacgg
      481 tcgctcactt cgcggatttg atcaaatgcc gtttcccacg cccaggtttt ccatacgcag
      541 ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc cagttcatcc
      601 aacccacgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc cataaactgc
      661 gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg acgtgcacga
      721 ctgtgtttcc acgcgccagc cagttcgtta atcagtcctt ccgcaggatt gaagccgttg
      781 cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt atgacaggag
      841 gcagaatcgg cgttgtattc gtagactttc aggccacgtt catccatgca gaaatccata
      901 cgaccagtga tcatatgatg acggcgacgc tgccaggaga gacgcaaacg tggccagagg
      961 attttcggaa tgtcaaacag cgccagcagg ttgtcatctt tcagcacctt gtcggtggcg
     1021 tgaagataca tcaggtgcag ctcgttggtg gctttaatca gctcctgctc ggcactctcg
     1081 gtaatggtgt agtaatgata cggatcctga ttgatcacct ggccgttggc ctgtacatag
     1141 gcgttttgca gcggatcttt ttcatccagc catttaccgt caaactggcc tttgttttcc
     1201 agacgcgccc cgctgatttt cagcagttcg cctgcaattt ccggctgtgg caagctgtat
     1261 tcggtatctt ccgtctggat catccagccc agaatagtgg tgtcatcgaa ggtgtctttc
     1321 agggtatagc cgccgttttc gaccaccatc tccagctcgc gtgtccactg ttgcccttgc
     1381 ggcaacgggg tatgaatcac gttctgttcc gcaatacgga ctttgttgcc atgcaattgg
     1441 gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca gataagaagc
     1501 gcccccgcta ccggtgcacg cggcgaaccg ttaggaaatg cctgcaatgg caggatgttg
     1561 tcattgacca cttcacgcag gaagcgcagt gagaaaatct cccatgccat acccacgtca
     1621 gtgaagacta caccgtagtt cagaaagaga aaacggcgag caaattcaac gcattgccat
     1681 ttgtggccca tatattcatc gtcgatatag ctacggaata cggcgtcatc ttcgtattcc
     1741 tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg ggcgtagccc
     1801 aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac tttaccttta
     1861 acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct cagcgacatt
     1921 aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc aaagggttaa
     1981 aaaattcaca ttctgtacag caagtgacct gctacactgc ttcaacacta ccactcagaa
     2041 ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg tgggataaat
     2101 ccgctggcgg cgcgttcgcc agtatcaatc gcccggtttc tggtccgacg catgaaaaaa
     2161 cgctgccggt gggcaaacat ccattgcaac tttattcgct gggaacgccg aacggtcaga
     2221 aagtaacgat tatgcttgag gagctgctgg cactgggcgt tactggtgca gagtacgacg
     2281 cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc gaagtgaacc
     2341 caaactcgaa gatcccggcg ctgcgcgacc atacccataa tccaccgatc cgcgtgtttg
     2401 agtctggatc gattctgctt tatctggcgg agaaatttgg ctacttcctg ccgcaggatt
     2461 tggcaaagcg tactgaaacg atgaactggc tgttctggtt acagggcgcg gcaccgttcc
     2521 tcggcggtgg ttttggtcac ttttaccatt acgcgccggt gaaaattgag tacgccatca
     2581 accgctttac catggaagcc aagcgcctgc tcgacgtgct ggataagcaa ctggcgcagc
     2641 acaagtttgt tgcgggcgat gagtacacca ttgcggatat ggcgatctgg ccgtggtttg
     2701 gcaacgtggt gttaggcggc gtgtatgatg ccgcagagtt tctggatgcg ggcagttata
     2761 agcacgtgca acgctgggcg aaagaagtgg gcgaacgtcc ggcggtgaag cgtgggcgta
     2821 ttgttaaccg caccaacggg ccgctgaacg agcagttgca tgagcgccat gacgccagtg
     2881 atttcgagac gaatacggaa gataagcgtc aggggtaagt cgcaacattg gcaggcttat
     2941 cgctatgtag ccggataaag catttacgcc gcatccggct ttaatcatca cgcgctggta
     3001 atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt tggcttcatc ttcgtcgatg
     3061 atgctcatgg caaatccgac gtgtaccagc acccactggc ccagcagatc ggcagggtta
     3121 ccttcacaaa tcagggcgat attcacgtcg cgcttgatac cacatacttc aacctgcgca
     3181 agctggtgaa tatcttcacc gacagccagc acctggcctg gaacgccaat acacattgtt
     3241 aactccagtt attcaacttc aatacttttg acgatcagcg aatcgccggt atcgacacgc
     3301 aaccgctcgc catgacagag cggacactgc gcatcgtgct gatgaatctc caccacctgg
     3361 ctgcaatccc agcaccaggc ctgggcgggt ttatagacga tatgtaaatc gcacccttgc
     3421 gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga cggcgctctc ctcaacgcag
     3481 gagagcgcgc caatttccag ccacacggcg gtgacgcgct taacatcgtg ctgctccgcc
     3541 tgccgttgga taatttcaac ggcgctctgg caaagcgaca actcatgcat tttcgccact
     3601 cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt acatccggat tagcgaccgg
     3661 tagcgaaagg atcatccgcg cgcagtcatc cgtcaggcgt tggccctctt caatcgacat
     3721 gctatgcgaa agcggtgaca tcagcgaaca ggagagatat tgagaaacac tgtccagttc
     3781 gccaacggta aaggtcatag tgccatacgg cagttgcaga ccaatttttt cactcacttt
     3841 gcgcagcggc cagagttgat ccgggccggg gaagataact gcactcagca tccacggggt
     3901 gatcacacac cccgtccact gaccttcgaa cagcgtaaag tcagaaacat acaccggcat
     3961 tgagggatgc agaaaagaga gatcgtgcat cgaacgccgg gcaatttctt caaacgctgc
     4021 ctgtacttgc gccttcgggg aggtctggaa acctgctatc tcttcagtca tgagtcgcct
     4081 cccgtgggat agcttccacg cctgattcgc gcagcgcagc cagaacctgc tcaagcgcag
     4141 gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg cggctccagc gattccggga
     4201 tcacaccgac cagcgtcagc tttttcggaa actcgccggt gaagcgcagg gccgacaata
     4261 cgtcggccag gccaagctga tgcggagaga ttttgttggt aaacaacgcc ggaacttctt
     4321 catcccgcag gatcatcatc gttcccggcg cgttcttttt cgacacaatg gcatccgcaa
     4381 taatcagatg atcgcgattt gccatgtcgc caagcagctc cattcccgcc gtgccgccat
     4441 cgaggatctc aacataatcc ggcagaatgt atcgttgctc taacgcttca acaatccgca
     4501 caccgatggc ttcatcggtc agcaaaatat tgccgacccc taagactaaa atacgcatta
     4561 cagaaccttc actgaaacca cttcgttgcc gtcggcatcc actacgtgta ccgcacaggc
     4621 catgcacggg tcgaaggagt gaatggtacg caccacttcc agcggtttat tcggatcggc
     4681 aaccggtgta cccaccagcg actgctcgta aggaccgacg tcatcattga agttacgcgg
     4741 accagagttc caggttgatg gaacaaccgc ctggtagttg ctgatgatac cgtctttaat
     4801 aaccatccag tgagagagca taccgcgcgg cgcttcgagg aagccaacac ctttgaactc
     4861 acccgttgcc ggaatgttcg gcttcacaaa ggtggtgtga tcgcctttgc cgatattggt
     4921 gatcagtgca ctgtattggt tttgcaggat atcctgcaat tcacagcagt gaacggtacg
     4981 accaataata cggcccagcg tggagtgcag ctgtgccact tccagcgtgt tgccagtcag
     5041 tttctgataa atcgcaacga tttcattcag tttgttctgg gtagattcgc gacctgccgc
     5101 cagtttcacc agcatattag ccagcggacc cacttctacc gttttgccgt agaaagtcgg
     5161 cgatttcacc caggaatatt taccgtcatc agaccaaccg tcgtaagccg gaatggtggt
     5221 accttcccac ggtgcctgcg gcgcttcgtc tttataccag gagtgcttcg cgctttcctg
     5281 aatgcctttg atcaggtatt catcggaatg agaagtgatc ggacgatacg aggacagatc
     5341 cgcattctca atgtagccgc ccgggaacag gaagctgccg tttttgctgt cggtcgggaa
     5401 ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc gtcagccatt ccgggtagaa
     5461 cgcggcgata actgcggtat caaccttata aacctgttca acaaagtcgc tcagcttgtc
     5521 gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc acgcccaaac cgtcgaggtt
     5581 gattgggtta gcgaccccgc ctaccgccag gttctgaatg tgcggcgttt taccgcccag
     5641 cagcgccacg acgcggttag cgtcacgctg gcactccagc gcctgcaggt agtgcgctac
     5701 cgcaatcagg ttcacttccg gcggcagttt catcgccggg tgaccccagt agccattggc
     5761 gaaaataccc aactgaccgc tggcaaccag atctttaatc ttgttctgaa ctttggtgaa
     5821 ctcttccgga ctgttcagat gccaggtcga aacgcctttc agcatttcag aggctttggt
     5881 tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc agcgccgaaa gctgatagaa
     5941 atgaacgata tggtcatgcg tggtgtgcgc agccagaatg atgttacgga tgtattgcgc
     6001 gttaaccgga acgtcgatat tcagcgcact ttctgccgca cgaacggaag acagcgcgtg
     6061 agtggtagta catacgccac agatacgttg cacaatcatc catgcatcgc gcggatcgcg
     6121 gtttttcacg atctcttcca tgccgcgcca catggtaccg gaagcccatg ctttcgaaac
     6181 gacgccattt tcgatttcgc aatcgatgcg taaatgcccc tcaatacggg ttaccggatc
     6241 aatagtaatt ctctggctca tgctttgctc gcctcatgac gattatgatc gttttgtttt
     6301 aaaggaggaa gtatcggcag tagacgaatg agtacgatgt aagcgcaaat ctcaatagcc
     6361 acaaaaccaa tagaaatcaa cagttcttcc caggtcggga agtaggcgta accgccgccc
     6421 gggttgaatg ccaccagcga ataggtcaga cgccaggttg cacaacctaa cagtgcgctc
     6481 agtgctgaca ggaacagcat gcgggaatca ttacgcagct tcgccacacg cagaacgacc
     6541 agcgggaaga gcatcagcag gacttcaatc cagaacatca cggagtagaa gtcaccggca
     6601 aacgctaacg acagcttgtc gcgatagata agctcgccaa agcgcagcac gatgaaaatc
     6661 gccagcaaca cactgatggt gttggtcagc ttgacgaaca ggctcttttc atccggaccg
     6721 ttgccacgca gacccgcctg caccagtgaa ccttcaaaga tgacaatcga gaagcccatg
     6781 atgaacgccg tcagcagcga gaacagcggc aacatttcat agctctgcca cagcggatgc
     6841 accttgtagc ccgccgagat catcagcgac cccattgaag actggtgcat ggtcggcagc
     6901 agcgcaccga gcgcgatgat gaagaacatc accttgttca gacgctgtag cgacaccttc
     6961 cagcccagac gttcaaacag tgccggagca aactccagtg ccatcacgcc gatatagatg
     7021 gtcatacaga ccgccgtctc gaacagtacc gagttcacgt tgaagtgacc cggaatgtag
     7081 aagtacggca ggttccagta gcgacccacg tcgatagtga tcgacaagcc acctagtgag
     7141 taaccaaaca gactcgccaa cagcgccgga cgcaccagcg gatggtattg cccacggtta
     7201 aagacgtata ccgcccacgc cagcgcccag ccgccacagg caaagccggt gccgatcaac
     7261 aggtcaaacg cgatccacac gccccacggg aagccgccgt tcaggtcaga gacagagccc
     7321 agaccgaaca ccagacgctt cacaatcagg agcatacaga tgacgattaa cggtccaaaa
     7381 atcatgaccg gtttactgat gattttgccg cccagcggtt gtggatcatg actcatgatc
     7441 gtctcctccg tcgtgatggt cgtttttggt gttgcgacga accagcacgg ttaagcccgc
     7501 cagcacagcc agtggtagca tcatgccttt atacagggtg tgttgaatat tttcggaacg
     7561 cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga ttttcataag gcacacccgt
     7621 cagtaccaga acctgagtac cgccgccctc tttctcgccg tacagatgcg gataatattt
     7681 cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc tgacgcggat agtggtattc
     7741 gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc atcagctctt cacgcgtacc
     7801 aaaaatcacc gcgcccgccg ggcacacttc tacgcagcca ggcagaccgc ctttatcgag
     7861 acgttccaca cctttctggt tgcacaactc gcacttatgc agcgcaccaa acgggttgtt
     7921 gtagtcgtac ttcggcacgt tgtatgggca ggcgaccatg cagtaacggc agccggtgca
     7981 cacatctttg tcgtaatgga caatgccggt tttcggatct tttttcagcg cagagaccgg
     8041 gcacacagag acacagttcg gatcgacgca gtgcatacac tgtttcttaa tgtacgcgta
     8101 gccattctcc tcctggtctt tgttgacccc tgtgccgctg gtccacacct gaatgatgtt
     8161 attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc tgttcccctt gcgggttacg
     8221 ttcaggggag ttgatatcct gacacttggt gacgcaagcc tgacagccta cgcacaaggt
     8281 cgagtcgtac aacatcccca gcgatcccgg aattggcggg cggttttcag cagccgcatg
     8341 actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag gaggctgctt taataaaatt
     8401 acgtctgttc acggttattc tccccgtgag tcagcgttat ctttcttttg ctgacgaccc
     8461 agttcacgca ccgccatcac gctgacaccg gcaaccagcc caaccactcc gccgagcaaa
     8521 ccaatagcgc ctgccgagac gttgccgcct tctttagcgt taacgtccgg tttctgtgaa
     8581 cgcggagtct ggttttcgac gttggcaagc tgatggatgc ctttatggaa gccgatacct
     8641 tcttcgttac agccatagca cgggtgacca atcgctaccg gccacacgcc gccaacatcg
     8701 cagaattgca gcgttgagca gttgccgtaa gtttctggcc ctttacagcc gaggtggtac
     8761 aggcaccagc cttcgcggtg gccttcatca ccgaactctt tggcaaaacg accagcatcg
     8821 aagtgcgggc gacgttcaca gtgttcgtga atcagacgac cataggcgaa agtcggacgg
     8881 tttttgtcat ccagtttcgg cggtttgccg taagtgatga tgtgcgcaac ggtcgccagg
     8941 aagttgtgcg ggttcggcgg gcagccagga atattgataa cggttttgcc cggtagaact
     9001 tcttgcaggc tgactgcgcc agttgggtta actccagctg cggcaacacc gccccacgca
     9061 gagcaggaac cgatagcgat aatggctgct gcgccttccg ccgctttgcg gatgtgatcc
     9121 acaatcggct caccggcaac catgcaataa ataccgttat cttttaatgg gatggaacca
     9181 tccaccacta acacatactg ccctttgtac ttctcgagag cgttatgttt gttctcttcg
     9241 acctgatgac cgaaggcggc ggaaagcact tcgtgatact ccagagagat agtctccagc
     9301 acgaggtttt ctaccgttgg atgcgttgca cgaagcagag attccgtaca accggtgcac
     9361 tcctgcgcgc caatccagat aactggcgga cgctgcgggt tagtaaccga ttcgaccatc
     9421 tctgcagcgg ctttgctact taaccccatg gtggcggcta atgctgcaca aagcttcatg
     9481 aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt tatctccagt catttatagt
     9541 tattccgttg cgaagacctg gcatatattt tgcctcaatc gcaaaatcaa taatgcgatc
     9601 gatgcgccat ttaccacaca tttattatgg ttatcgatat catgatactg cggcggggca
     9661 ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag caagataata cgtatgtttg
     9721 atcaattttc taggcgataa ccggccataa aaaagcgcaa cgtagaacag gaattattct
     9781 cacgaaagaa atccattacc atctctttat ccagtccata acggcgcgtc agaatacccg
     9841 cttcccaggc gctaagctgg cgatctccgg tcttttcgta catccggtgt gagtagccta
     9901 atacaaaacc gcgtttataa tcggcacaga attttgtcac attaaccgca ctgtcggcat
     9961 gcgttgcttt taatcccgcc attaagcctt taccaaaatg gttattcatc gttcacctca
    10021 ttcgctatat attgtcatat atagcgatat tttaggcaaa gaacgacatt tgcgcacgct
    10081 attttttcat gcttgtttaa gacgtaatcc tgtaagagat aaacccatca ggagagatat
    10141 ttatcgaacc atttcagcgt cctttgccag gcaagatcgg cggcagattt gtcataacgg
    10201 ggcgtggaat cattatggaa tccgtgatta acccccggat atatatacgc ctcatagacc
    10261 ttattattgg ctttcaacgc cgcctcgtaa gcgggccagc cctcgttgat tcggctatcc
    10321 agttcagcgt agtggagcag taaaggcgct tcaatcttcg ccacatcggc agtgggtgcc
    10381 tgacgcccat aaaacggcac cgcgcaggcc agttccggat acgcgactgc tgccgcgttc
    10441 gatacgcccc cgccatagca aaatccgcta atacccactt tgcctgtcgc ttgcggatag
    10501 cgttgcataa actcaattgc ggcaaagaaa tcattcatca gtttggttgg atcaacctgc
    10561 tgttgcagtt cgcggccttt atcatcgtta ccaggatagc cacctactga gcttaaaccg
    10621 tctggcgcca gggcgatgta cccagccttc gccactcgcc gcgtcacatc ttcgatatac
    10681 ggattcagcc ctcgattctc atgcaccact accacggctg gcgttttgcc gctcattttt
    10741 gcgggcttca ccagataacc ccgtacctcg ccgtgaccat ttggcgaggg atacgtgatg
    10801 tactcagcaa caatttccgg gtcggtaaac tctacctgag tcgccaacgc ataatttgac
    10861 ttgagcaaat caaacagcgc taatgccgtc atcccgccca ccgcatactt cgccgcaagg
    10921 ttgaggaact cacgtttcga gattttcccg tgagcgtaat agtcgtagta atccagcaac
    10981 tcttgtggga aatctttggc ggttaaacgc ggcatcgttg cactcctcaa ctggtgtttt
    11041 tttaagcaaa gcataagcgc gtgtttttgc ccagtttttc gtcactctgt gagccagact
    11101 acgggatacg cgctggcgaa tcgctaaact agaaacattg tttcgaaatt gaacgatgga
    11161 aaggagaggt catggtctgg ttagcgaatc ccgaacgtta cgggcagatg cagtaccgct
    11221 attgcggaaa aagtggttta cgcctgcccg cgttatcgct cggtttatgg cacaatttcg
    11281 gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg taaagcgttt gatttaggca
    11341 ttacgcactt tgatttagcc aacaactacg ggccgcctcc aggaagcgca gaagagaact
    11401 ttggtcgcct gctgcgcgag gattttgccg cttatcgcga tgaactgatt atctctacca
    11461 aagccggata cgatatgtgg cccggcccat acggctctgg cggctcacgt aaatacctgc
    11521 tcgccagcct cgaccaaagc ctgaagcgta tggggcttga gtatgtcgat atcttttact
    11581 ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc ctctgcgctg gctcatgctg
    11641 tacaaagcgg taaggcgctg tatgtcggga tctcctctta ctcgccagag cggacgcaaa
    11701 aaatggtcga gttgctgcgc gagtggaaaa ttccgctgtt aattcatcaa ccttcgtaca
    11761 atttactgaa ccgctgggtg gataaaagcg gcctgctgga taccctgcaa aataacggcg
    11821 tgggctgcat tgcctttact ccactggcac agggattgct gaccggaaaa tatctcaacg
    11881 gtattccgca agattcacgg atgcatcgtg aagggaataa agttcgtggt ctgacgccga
    11941 aaatgctcac cgaagccaac ctcaacagcc tgcggttatt gaatgaaatg gcacagcagc
    12001 gtggacaatc aatggcgcaa atggcgttaa gctggttgct gaaagatgat cgcgtgacgt
    12061 cggtattgat tggtgccagc cgcgcggagc aacttgagga gaacgtgcag gcgctgaata
    12121 atctgacatt tagcaccgag gagctggcgc agattgatca gcatatcgcc gatggcgagc
    12181 tgaatctgtg gcaggcgtct tccgataaat gacctgttaa taacgggcgc gagaaccgcg
    12241 cccgaatcag atcagtgatt atgacgagtc agtcggtcaa gatagcccat cacaaatgca
    12301 gagagcacaa acgtcagatg gataatgacg taccacatca gtttgttatc cgggacattt
    12361 ttcgcatcca taaagacgcg cagtaagtga atggaagaaa ttgccacaat cgacgctgct
    12421 actttgtttt tcagcgacgt tgcatccatt ttccccagcc agttcagctt ctctttgttc
    12481 tcggagatat ccagttgtga gacgaaattc tcgtaaccgg aaaacatcac catcaccagc
    12541 aaaccgccaa ccagcgtcat atccaccagc gacagcaaca cgaggatcaa atctgattcc
    12601 gccatcgaga agatattcgg cagtacgtga ataatctcct ggaagaactt cagcgccagg
    12661 gcaactaacg ccagcgaaag gccaaagtac acgggggcaa gcagccagcg agaagcatac
    12721 attgcatttt caagaaaacg ttccatacat tcctgtcgtg tagttaaaac gagcaacagt
    12781 atatcgcaat taagtaaaca gcacgcaaca gcaaatcaac atatacgtta aacctttgtc
    12841 gcataattct gagggttagg gatcttcctg gttcccttat caacgcatcg agagcgtcta
    12901 cccttaaagg acgttgaacc tgaagggaga aaaatgatgt ctcatttaaa agacccgacc
    12961 acgcagtatt acactggtga atatcccaaa cagaaacaac cgacgccagg catccaggcg
    13021 aagatgacac cggtaccgga ttgcggcgag aaaacctatg ttggtagcgg tcgcctgaaa
    13081 gatcgtaaag cactggtgac agggggcgat tccggaatcg gtcgcgctgc cgccattgct
    13141 tacgcgcgtg aaggggctga cgtggcgatc agttatcttc ccgtggaaga agaagacgct
    13201 caggatgtga aaaagatcat tgaagaatgc ggacgcaaag ccgttctgct gccaggcgat
    13261 ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag cacacaaggc gttaggcggg
    13321 ctggatatta tggcgctggt agccgggaaa caggttgcca ttcctgatat tgcagacctc
    13381 accagcgaac agtttcaaaa gacctttgcc attaacgttt tcgcgttgtt ctggctaacc
    13441 caggaagcga tccccctgct accgaaaggt gcaagtatca tcaccacttc ctcaatccag
    13501 gcataccagc caagcccgca tttactggac tatgcggcta cgaaggcggc aattctgaac
    13561 tatagccgtg gcctggcaaa acaggtcgcg gagaaaggta ttcgggtgaa tattgtcgcg
    13621 ccaggcccga tctggacagc actgcaaatt tctggcggac aaacgcagga taagatcccg
    13681 cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac cggcggagct ggcccctgta
    13741 tatgtttacc tggcaagtca ggagtcgagc tacgtcaccg cagaagtgca cggtgtctgc
    13801 ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa aagcaaccgg gcatcatttt
    13861 tatttcgctt tcgcagtttc ttcgccgacc agacctattt tcaggtaacc cgcctgatgc
    13921 agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttatccgc tcggaagaag
    13981 atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg
    14041 gtgaccggat cgttaccaat aaacatcgag ttgtctgctt tcaccgacag ataaaccggt
    14101 ttttccgggc gcggctgcgg cgtactggta gaagcaggca aattcacctt cacatctacc
    14161 gtcgctaacg gtgccgccac cataaagata atcagcaaaa ccaacatcac gtcgataaac
    14221 ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt aagatgcatc
    14281 gccatcggac attatcctgc gcgtaatttt tgtgcgacac gaaccggatg tgcagcggcg
    14341 ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc aacatcaccc
    14401 agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac gaccgcagga
    14461 atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcaatacc cggcgcaacg
    14521 actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat gatgccccag
    14581 accgtaccaa acagaccaac gaacggcgaa atcgcgccga tggttgccag atagccgtta
    14641 ccacggccca tttgacgacc tactgcggcg acccgacgct ccagacggaa gctggtacgt
    14701 tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca
    14761 ttgagcaaat gcaggctcaa gcttttgcta ccaaaatcag cggcgatatc gttggcctgg
    14821 tttaaggaac gcgcttcagc cagcaattgc tgctcgcgct taagacgacg cttctgattg
    14881 aagaactcca cgctcttact gaagaagatt gcccaggtga ctacggaggc caaaataagc
    14941 ccaatcatca cgcacttaac gacaatatcg gcgtgctgat acatacccca gacggagagg
    15001 tccgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca caaaatctgt
    15061 gcataataat atcaaaacga cgtcgaattg atagtcgttc tcattactat ttgcatactg
    15121 ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt
    15181 tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata ttcatgctag
    15241 tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa agcttgatac
    15301 tcaactggtg aatgcaggac gcagcaaaaa atacacgctc ggtgcggtaa atagcgtgat
    15361 tcagcgcgcg tcttcgctgg tctttgacag tgtggaagcc aaaaaacacg cgacacgtaa
    15421 tcgcgccaat ggcgagttgt tctatggacg gcgtggaacg ttaacccatt tctccttaca
    15481 acaagcgatg tgtgaactgg aaggtggcgc aggctgtgtg ctatttccct gcggggcggc
    15541 agcggttgct aattccattc ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa
    15601 caccgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac
    15661 gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc agccaaacac
    15721 taaaatcgtg tttctggaat cgccaggttc catcaccatg gaagtccacg atgttccggc
    15781 gattgttgcc gccgtacgca gtgtggtccc ggatgccatc attatgatcg acaacacctg
    15841 ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta ttcaggccgc
    15901 caccaaatat ctggttgggc attcagatgc gatgattggc actgcggtgt gcaatgcccg
    15961 ttgctgggag cagctgcgag agaatgccta tctgatgggc cagatggtcg atgccgatac
    16021 cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgcc aacatcatga
    16081 aagcagtctg aaagtggctg aatggctggc agaacatccc caagtagcgc gagttaacca
    16141 ccctgctctg cctggcagta aaggccacga attctggaaa cgagacttta caggcagcag
    16201 cgggctattt tcctttgtgc ttaagaaaaa actcaatgat gaagagctgg cgaactatct
    16261 ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat cgttgatcct
    16321 ggcaaatcaa ccagaacata tcgccgccat tcgcccacaa ggcaagatcg attttagcgg
    16381 gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg ccgatctgga
    16441 cgccggtttt gcgcgaattg tataacattg ccacttttgg acaattttgc agacatttta
    16501 ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga ttcaggagta
    16561 caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct gttattcaag
    16621 atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggaccct catattgtta
    16681 gcgttgttta ctttgttatg tttgccacgc tgtttttaga aaacggcttg ctgcccgcct
    16741 catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc cagggggtta
    16801 tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc tgctggctaa
    16861 gttatattca ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg ctggcacagc
    16921 ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt ctgctggcgc
    16981 tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg gcgggaattt
    17041 ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt aagcggattg ctgtgggtca
    17101 gcgtggtaac cagtttcggc tatgccttaa gtatgattcc gttcgttaaa cgacatgaag
    17161 atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct ggcttgttgg
    17221 gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt tccccgttcc
    17281 cggttgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct tgccgcatct
    17341 tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga tgcgctttca
    17401 tagccgacgc gcatcgccgc cgcgctggcc ttcatgccgt cgtggatgat catcatccgc
    17461 gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt gacagactta
    17521 aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg ctcgacgctc
    17581 aggttttcgg tgtatttatt ctcaatccgt ttcagcacgc gactaatcag gctgaagtga
    17641 gtctggcggc tgaccagtgc cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac
    17701 agaatttcgc ggataatctg tttgccgaga atgcgcgcat ccagcggtcg ctccatcaca
    17761 tccagtaacc gctctgccgc gcataaaatc tcttctgata acgtggcgga gttaataccg
    17821 ctggctgcca ttgacggctg aaaatgttca tcttcgccaa tgtccatcaa cagttcctgt
    17881 aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga cgtggcatag
    17941 gtttcgcact caaacggcaa cggcaccgtc agcaggagat attcattagc gtcataacga
    18001 aacacgcgtt cattgatata accgatttta tgtccggaaa agagaattat gatgccaggc
    18061 tcgtacatca ccggtgtgcg tgcgaaaggc gtctcgccat acaacaaacg cacatcgggc
    18121 aacagctctg acaaactatt ttctttattt ttcagtttat taactttatc cgccagcaag
    18181 cggcaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg gcaatttgat
    18241 tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga cattggcaga
    18301 aatgagcatt gagaggtacg gcgctggcga ccacaatgaa aaacatcagg cagaccgatc
    18361 tctgccctca tattgaccca gcaaagggag caagtaatga acaactttaa tctgcacacc
    18421 ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca aattcctcac
    18481 gatgctcgcg tattgattac ctacggcggc ggcagcgtga aaaaaaccgg cgttctcgat
    18541 caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat tgaaccaaac
    18601 ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacaaaa agtgactttt
    18661 ctgctggcgg ttggcggcgg ttctgtactg gacggcacca aatttatcgc cgcagcggct
    18721 aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggcaa agagattaaa
    18781 agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga atccaacgca
    18841 ggtgcggtga tctcccgtaa aaccacaggc gacaagcagg cgttccattc tgcccatgtt
    18901 cagcccgtat ttgccgtgct cgatccggtt tatacctaca ccctgccgtc gcgtcaggtg
    18961 gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac caaaccggtt
    19021 gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctgat cgaagatggt
    19081 ccgaaagccc tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat gtgggcggcg
    19141 actcaggcgc tgaacggttt gatcggcgct ggcgtaccgc aggactgggc aacgcatatg
    19201 ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact ggctatcgtc
    19261 ctgcctgcac tgtggaatga aaaacgcgat accaagcgcg ctaagctgct gcaatatgct
    19321 gaacgcgtct ggaacatcac tgaaggttca gacgatgagc gtattgacgc cgcgattgcc
    19381 gcaacccgca atttctttga gcaattaggc gtgccgaccc acctctccga ctacggtctg
    19441 gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat gacccaactg
    19501 ggcgaaaatc atgacattac gctggatgtc agccgccgta tatacgaagc cgcccgctaa
    19561 gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag ttcacaacac
    19621 ctcaccggag cctgctccgg tgagttcata taaaggagga acgtatggct aatccaaccg
    19681 ttattaagct acaggatggc aatgtcatgc cccagctggg actgggcgtc tggcaagcaa
    19741 gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat cgctcgattg
    19801 ataccgccgc agcctacaag aacgaagaag gtgtcggcaa agccctgaaa aatgcctcag
    19861 tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac aagcgccccc
    19921 gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac ctctatttaa
    19981 tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc atgatcgaat
    20041 tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc catcacctgc
    20101 aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa cttcacccgc
    20161 tgatgcaaca acgccagcta catgcctgga acgcgacaca caaaatccag accgaatcct
    20221 ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt cgcgatctgg
    20281 cagataaata cggcaaaacc cctgcgcaga ttgttatccg ctggcatctg gatagcggcc
    20341 tggtggtgat cccaaaatcg gtcacacctt cacgcattgt cgaaaacttt gatgtctggg
    20401 atttccgtct cgacaaagac gaacttggcg aaattgcaaa actcgatcag ggcaagcgtc
    20461 tcggtcccga tcctgaccag ttcggcggct aacgtgcaaa ttatcccggt ggcggtaatg
    20521 ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc gttgctactc
    20581 ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga agctcttaaa
    20641 agaacaaccc atctatatcc gggataaacg caacggagag gtgaaaaaca gatgaaaata
    20701 atacttctgt ttttagcagc cctggcaagt tttaccgtac acgcacagcc cccctcacag
    20761 accgtagaac aaacagtccg gcatatttat cagaactata aatcagatgc cactgcccct
    20821 tattttggtg aaaccggaga gcgagcgata acttctgcgc gtattcaaca ggcgcttatc
    20881 ctgaacgaca atcttacgct gccgggcaat attggctggc tggattatga tccggtttgt
    20941 gattgtcagg attttggcaa tctggtgtta gaaagcgtag cgataaccca aactgacgcc
    21001 gatcatgccg atgccgctgt gcgctttcgt atctttcaag acgataaaga aaagacctcg
    21061 cagacactga aaatggtggc ggaaaatggt cgttgggtca ttgacgatat tgtcagcaat
    21121 catggcagcg tcttacaagc ggttaatagc gagaatgaaa aaacgctggc cactatagct
    21181 tcgttgcaaa aagaacagcc ggaagccttt gttgccgaac tctttgaaca tattgctgat
    21241 tatagctggc cgtggacgtg ggtggtttcc gactcttacc gccaggcggt caatgccttc
    21301 tataaaacca ccttcaagac ggccaacaat cccgatgaag atatgcaaat agaacggcaa
    21361 tttatttacg acaatccgat ctgttttggc gaagagtcgt tattttcccg cgttgatgaa
    21421 attcgagtcc tggagaaaac caccgattcc gcccgcattc atgttcgctt tacgctgacc
    21481 aatggcaaca acgaagagca agaactgatt ttacagcggc gcgaaggtaa gtgggaaatc
    21541 gctgatttta tccgcccgaa cagcggcagc ctacttaagc agattgaggc aaaaaccgcc
    21601 gccagattaa aacaatgagc tgaattaaat aacaattagc cggaacaata aataaaaggg
    21661 aacactatat gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc tgcttgctca
    21721 gtggctgcgt caatgagcaa aaggtcaatc aactggcgag caatgtgcaa acattaaatg
    21781 caaaaatcgc ccggcttgag caggatatga aagcattacg cccacaaatc tatgctgcca
    21841 aatccgaagc taacagagcc aatacgcgtc ttgatgcaca ggactatttc gactgcctgc
    21901 gctgcttgcg tatgtacgca gaatgataaa aaaatccccg gcagcattct gttaccgggg
    21961 atatttttta acgtccaacc gccgctttag ggcgtttctt cgcaccagca ttctgcaggc
    22021 gagattgcgt agacgacgct ttttttgccg tagcaggcgt ctgacgctgg gtcgccatcg
    22081 gcgtatgttt agtcaacgcc ggacgggtat tgcggttctg gcgacgagcc tcacgcatct
    22141 cttcaatagt tggcgcaggc actaagcaat cgcgacggct gccaatcaga tgctttttgc
    22201 ccatcgcttc cagcgcctgg cggattaacg gccagtttgc cggatcgtgg taacgcaaca
    22261 acgctttatg caaacgacgc tgtttgtcgc ccttcggcac gaagacgtct tcactcttat
    22321 aaccaatctt cgccagcggg tttttgccgg tgtaatacat ggtggtcgag ttagccagcg
    22381 gcgacggata gaagttctgc acctggtcga ggcggaagcg atgctttttc agccatagcg
    22441 ccagattcac catatcttca tcacgcgtac ccgggtgcgc ggagatgaaa tacgggatca
    22501 gatactgttc tttacctgcc tgtttcgagt aagtatcgaa cagctcttta aagcggtcat
    22561 agctgcccat gcccggcttc atcatcttcg ataacggccc ttcttcggta tgttccgggg
    22621 caatcttcag ataaccgcca acgtgatggg tcgccagctc tttgatatag cgcggatctt
    22681 ctacagcgat gtcataacgc acaccagagg caatcaggat ctttttaatg cctttcagat
    22741 cacgcgcacg gcgatagaga ttgatggtcg gttcatgatt agtgtccatg tgcgaacaaa
    22801 tatccggata aacgcacgac aaacgacggc aggtttgctc agcgcgtggc gatttgcagc
    22861 gcaacatata catgttggca gtaggcccac cgagatcgga aatcacgccc gtaaaacctg
    22921 gaacagtgtc gcggatcgct tcgatctcat taataatcga atcttcggaa cggctctgaa
    22981 taatgcgccc ttcgtgctcg gtaatagaac agaaagagca gccgccaaag cagccacgca
    23041 taatgttgac cgagaaacga atcatttcgt aagccggaat tcgggcatta ccgtaagccg
    23101 gatgcggcac gcgcttgtac ggcagcgcaa agacgctatc catctcttcg gtagaaagcg
    23161 gaatagcagg cgggttgatc catacatagc ggtcgccatg tttttgcatc aatgcgcggg
    23221 cgcagcctgg gttagtttcg tggtgcagaa tgcgcgaagc atgggcgtac agcactttat
    23281 cgcccttcac tttctcgaaa gaaggcagca acacgtaggt tttttcccac ggtttcgggc
    23341 gtggtggctg cacggttaca gatttggctt cctgcttttt cggagctacc ggtttgttat
    23401 ccgcgcacgg caaatcttcg ccatacggat gcgggattgg gtcgattttc ccaggggtat
    23461 caagacgggt ggaatccaca ccgctccagc ctggcaacgc ctcttttacg ataatcgcgg
    23521 tattacgcac atcgcggatt tcactaatcg gctcgcccat cgccagacgg tgcgccactt
    23581 ccaccagcgg acgctcaccg ttaccaaaca tcagcatgtc ggctttcgaa tccaccagca
    23641 cggaacggcg cacggtatcc gaccagtaat catagtgcgc ggtacggcgc aggctggcct
    23701 caataccgcc gaggatcacc ggcacatctt tccacgcctc tttacaacgt tgggtataaa
    23761 ccagtgtggc gcgatccgga cgcttacccg cgacattatc tggcgtgtag gcatcgtcat
    23821 gacgtaaacg gcgatcggcg gtatagcggt tgatcatcga gtccatgttg ccagcagtaa
    23881 caccgaaaaa cagattcggt ttacccagac gcataaagtc gtctttgctg ctccagtctg
    23941 gctgggcgat gatcccgacg cgaaagccct gcgcttccag catacgaccg caaatcgcca
    24001 tcccgaagct tgggtgatcg acatacgcgt cgccagtaac caaaatgatg tcgcagctat
    24061 cccagccaag ttgatccatc tcttcacgtg acatcggcaa aaacggtgcc ggtccaaaac
    24121 aggcggccca gtactgcggc caggagaaca ggtcgcgatc cggttggatc agggagatag
    24181 agctcatttt gcttccagaa atggtaaaaa aataatcaaa ggccggggat tatacgctgt
    24241 atcacgcgcg gaaatgaagt gatgttgatg aacggtggcg aaataccctg gtgaggtttt
    24301 tcactgcccg acttgggcaa gcacatttaa ggctggcggt atcgtggcaa ctgccgctac
    24361 tatttatgcc gatcagcaac gttcaaatca tattgcacga cgatcggcta tccgaggaca
    24421 cccacagtaa ggcaaggtat ggaacaggtt attacaaagc gccgctatta cgatattggt
    24481 ctacagattg aagagctgct gtattcaggc gtattcaaag ccggagaacg cttaccttca
    24541 gaacgtgaac tgagcgagcg gtttaatacc agccgaacta ccattcgtga agccatcatt
    24601 atgctggaac tgaaaggcgt attgaatgtg aagcaagggt cagggatttt ttttgtcgac
    24661 tccaccgata agctcaacca gaaatcatta atgccctatt cagaaattgg cccgtttgaa
    24721 ctgttgcagg cgcgtcaggt gattgagagc aatattactg gttttgcagc cagccagatt
    24781 agctttaacg agttacaaga gttaaagaaa atcattagtt tgcaggaaaa cgctatcgct
    24841 gcggaaagtg atagatttga agacctggat catcgttttc atagcattat tgccgaagcg
    24901 acgcaaaatc gggtactcat caaacaagcc gctgaattat ggcgggcagt gcgtacagaa
    24961 aacccccgct ggaagaaact taactacaag tacctgcatg aaaagcattt gcgtctgcaa
    25021 tggctggaag atcaccgggc tattttcctg gctctgcaac agaaagattc agagctcgcc
    25081 agggaagcgt cctggagaca tctggaaaac agcaaaaatg aactgatcaa aatcttcaaa
    25141 caagacgctt ccatcagcga ttttgatgac tttttcttcg ctcgttaacc ttcattagtc
    25201 cagagctggc gggaaaaata acgccggctc tgcctttcgc cctccactgg tacacgctct
    25261 ctttttaatt ggtctaataa atcgactatc atcagatatc ttagattagc ggcaattatc
    25321 ctaaaaactg acctctaaaa aactttcctt tcattttatt catataactc attgatacag
    25381 atataaataa cgccttgttg ttctgtttcg tacttttacc tttctctgca agcgtgatgc
    25441 atctctctgt ttttggtcta gtgaattatg taaattggtc tacacacaag tcgtcaggag
    25501 taagagcata atgtcaggga acgaaagtat cgctgccgcc cactatgatc aggtaaccac
    25561 ctggcctcgg gacgggttac aggcagatat tgtccatatt ggttttggcg cttttcatcg
    25621 cggacaccag gctgtctaca cggatctcac taatcaactt tcggacaccc gctgggggat
    25681 ctttgagatc aacctgtttg gtgatgctca actgatcgaa aacttaaatg cgcaaaatgg
    25741 gctgttttcg gttgtggaaa catctgcatc gcaatctacc tcacgccttg tgcgttcggt
    25801 ggctggcggt attcataccc ccagagatgg cattgccgca gcaatccata aactggctga
    25861 acctcaggta aaaatcgttt cattaaccat caccgagaaa ggttattgcc tcgatccgca
    25921 aacacgttca cttgatctca ccaatggatt aatccaacac gatctacaaa acccggatgc
    25981 gcctctgtca gctattggcg tgatcgtctg tgctttgcaa caacgtaaag cggcaggact
    26041 cgccgctttt agtgtgctct cctgtgacaa cttgccagac aatgggcatc tgacgcgcaa
    26101 tgccgttctc ggttttgccc gacaactgga ccagcccctg gctcagtgga tcgaagaaaa
    26161 tgtctcattt ccaggcacga tggtagatcg cattgttccg gcaatgactg aatcgcaatt
    26221 cgccttactg gaaacgaaaa ccggttatgc cgatccctgc gggatcgtct gcgaatcatt
    26281 tcgtcagtgg gtgatcgaag ataattttgt gcgcggacga ccggaatggg ataaagccgg
    26341 cgcgatgttt gtcagcaatg ttcagcctta tgaagagatg aagttacgca tgttaaatgg
    26401 tagccattca tttctggctt ataacggctc tttggcaggc tatgagttta tctggcaatg
    26461 catggaagac gctaattttc gttccattac ccaccaactg atgattaatg aacaagcccg
    26521 aacacttaat ccagacttaa atatcaacat ccaggaatac gccgacctgt taattgaacg
    26581 ctttagcaac cgtaacgttg cgcaccgtac cgggcaaata gccatggacg gttcacaaaa
    26641 gcttccccag cgtgcactga cgccctggct gaaattacat cagcaaaagc aaaacaatgc
    26701 tgttctgtca ctgctcgttg ctggttggtt gcattatgtc attgatgttg ttgagaaaag
    26761 ccagtctgtc gctgatccaa tgaatgacca atttcaggcg ctaataaagg aacaacaaga
    26821 cgcatggcaa caggcgctcg cattactgca ccttagcgcc atatttggtg atttaagcaa
    26881 ccatcagcca tttataaatg aaataaaaat cgcctttgca aatataaaaa acaaaggcat
    26941 caaggccacc atcagccaat tattatcgga tgagcagaaa tgaaaacatt aatttgtcag
    27001 cagcctggcg ttatggaata tgtggaaaag gatattccca caccagcaga taatgaagtg
    27061 ctgttaaaaa tcaaagctgt gggtatttgt ggtactgata ttcacgcttt tgccggcaga
    27121 cagccttttt ttagctaccc acgtgtatta ggtcatgaaa tatgcgccga agcggtttcg
    27181 cgaggcagcc agtgccaaac agcacaatca ggccagcgct attccgtcat cccatgcatt
    27241 ccatgtggcg agtgcgcagc ctgtcgggaa aagaaaacga actgctgcga acgtgtttcg
    27301 ctgtatggcg tgcatcagga tgggggcttt agtgagtacc ttgcggtacg tgaagacaac
    27361 cttgtgcctc tccctgacga ggtcagcgac agtgccggag cattggttga atgtttcgcc
    27421 attggtgcac atgccgttcg tcgggcagag atcaaggctg aacaaaacgt actggtgatt
    27481 ggcgctgggc caatcggtct ggctaccgca gccatcgcca gggctaaagg ggcgcatgtt
    27541 gttgttgctg atattgactg tcaacgtcgc cagcacgttg tggatcatct ggcaattaat
    27601 gtcttcgacc caacacagga aggttttatt gccgcgctta gtgaagtatt tggcggcgaa
    27661 ctggcttgcg tagtcctgga tgcgacggga aataaagctt caatgagtca tgatgtaaat
    27721 cttattcgtc atggcggcaa aattgttttc atcggtttgt acattggtga acttgttatt
    27781 gacgatccga ccttccataa aaaagagaca acgttactca gcagccgcaa tgccacacgg
    27841 gaagattttg cgttggtgat tgaactgatg cgcagcaata aaattcacga aaatttaatg
    27901 aaaaaccagg cgttcaattt ctttagtgtt ggcgaagatt accagcgtaa cgttgtagaa
    27961 aataaaaata tggtcaaggg tgtgatcact ttttaaggga acatatatga caactgttta
    28021 tgtcagtgaa gaaaatttaa aatcactggt gcaccacaaa ttacataccg ccgggctgga
    28081 tgccgacact gcacaacagg tgacagatgt tttaatacat gccgatatta caggtgtgca
    28141 ttcacatgga gttatgcgtg tcgaacatta ttgtacccgc cttgctgccg gagggttaaa
    28201 taaagccccg cagtttagta ttgagcaaat ttcaccgtca gtggccattc tcgactctga
    28261 cgacggaatg gggcattcgg cattaattag cgccactgag cacactatta aactggccct
    28321 ggaagagggt ctcggttttg ttagcgttaa aaatacgtct cactgtggcg cgttatctta
    28381 ttttgcagag atggtcacca acaaagggtt ggttgctatc gtaatgacgc aaaccgatac
    28441 ctgcgtggct ccccatggcg gcgccgagcg ctttttgggt actaacccca tcgcctttgg
    28501 tttcccggtg gaaaacagcc atccgatgat tgttgatatg gcaaccagtg ccacggcttt
    28561 tggcaaaata cttcatgcaa aggaaacagg aaaacatatt ggtgaagggc tggcgataga
    28621 taaagacggt tatggcacca ctgatccgca taagattgaa aacctgctac ctttcggcca
    28681 acacaaaggt tcaggcattg cgctggctat tgatgcactg actggcatgc tgatgaatgc
    28741 gaattttggc aaccatattg ttcgcatgta tggtgattat gacaagatgc gtaagctggc
    28801 aagtttggtt attgccatcg atccgaaaaa gctcggcaat cctgtttttg caaaaaccat
    28861 ggcgcaaatg gtcacggagt tgcatgccgt taaaccggct cccggtatcg aaaaagtgtt
    28921 agcgccgaac gatccgcaaa tgcactacaa agaaaaatgc caacaggaag gtattccggt
    28981 tccagcagga atattccatt atctggcaga gaattaatca ctacttttta ttataacgat
    29041 agaaaagcgc tgtatttatc gtttctgtta tattaaccag gagcaggata tgaaagcact
    29101 tcgtcctctg acggcatcgc ttatgctgtt aaccagttgt ttattaatat ctaataccac
    29161 actggcaaaa acaacattaa aactgagtca taatcaggat aaaagccatg ctgtccataa
    29221 agcattaagc tatttagcgg ataaaaccaa agagtattct aatggtgaat tagttattcg
    29281 catttaccca aatgcgacat taggcaatga gcgtgaatca ctggaattaa tgaactccgg
    29341 tgcattacag atggttaagg ttaatgctgc atcactagaa tcatttgccc cggattacag
    29401 ccttttcagt ctgccgttct tattccgcga ccgtgatcac tattatcgtg ttctgcaaag
    29461 tgatttaggt aaaaaaatac ttaagtcatc agaaagcaaa ggttttgttg gtattacgta
    29521 ctatgacggc ggtgcgagaa gtttttattc caataaaccg attacaaaac ccgaagattt
    29581 agcgggaatg aaaatcaggg ttcaacaaag ccccagcgcc attgcaatga tgaaagcact
    29641 cggtggtgtc gctaccccga tggcgcaagg cgaactctac accgcacttc agcaaggagt
    29701 ggttgatggc ggagagaaca ataccgttgt ctattccgat atgcgtcacg ccgaagtcgc
    29761 aaaagtctat tcacgtgatg aacacaccat ggtacctgat gtgttaatta tcagcaccga
    29821 cgtgttgaat aaacttggtg ataaagagcg cacggcatta ttaaaagcag ccgatgagtc
    29881 aatgatgcag atgaaggacg tcatctggcc tgcggctgaa aaagaagcct acgacaaaat
    29941 gaaagggatg aacgcaacag ttgttgatgt tgataaatcc gctttcaaag aacgcgtcaa
    30001 accgttatac gatgaattca aggcgaaaga tgcacaatca gccaaaaacc ttgagctagt
    30061 agaaagtatg taaccctaat gggcaggtaa ttcctgcctt ttcatggaag acttatgcga
    30121 acactcactc atattcttaa taagatcctt gccggatgtt gctgcattat tctcgccatt
    30181 atggtgttct gcgtaacctg gcaagttatt gcacgtttta tttttaacgc tcccagcact
    30241 gtgcttgatg agttcaccca gattttattt atgtggatga ttttacttgg cggtgtatat
    30301 actgcggggt taaaaaaaca tctggccatt gatttgttgg ctcaaaaact tccggctgca
    30361 tctgttctga cgcttgacag ttttatccaa attattatta cggtctttgc ggttattttc
    30421 atgatctatg gcgggaatat tgttgttgaa aaagcaca
//
LOCUS       DACADF010000047        29788 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.047, whole genome shotgun sequence.
ACCESSION   DACADF010000047 DACADF010000000
VERSION     DACADF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.047"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..144
                     /locus_tag="ISB30_002002"
     CDS             <1..144
                     /locus_tag="ISB30_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP1989634.1"
                     /translation="VEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAK
                     VLS"
     gene            374..757
                     /gene="secE"
                     /locus_tag="ISB30_002003"
     CDS             374..757
                     /gene="secE"
                     /locus_tag="ISB30_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP1989635.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            759..1304
                     /gene="nusG"
                     /locus_tag="ISB30_002004"
     CDS             759..1304
                     /gene="nusG"
                     /locus_tag="ISB30_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP1989636.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1463..1891
                     /gene="rplK"
                     /locus_tag="ISB30_002005"
     CDS             1463..1891
                     /gene="rplK"
                     /locus_tag="ISB30_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP1989637.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1895..2599
                     /gene="rplA"
                     /locus_tag="ISB30_002006"
     CDS             1895..2599
                     /gene="rplA"
                     /locus_tag="ISB30_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP1989638.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     IRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2891..3388
                     /gene="rplJ"
                     /locus_tag="ISB30_002007"
     CDS             2891..3388
                     /gene="rplJ"
                     /locus_tag="ISB30_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP1989639.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3455..3820
                     /gene="rplL"
                     /locus_tag="ISB30_002008"
     CDS             3455..3820
                     /gene="rplL"
                     /locus_tag="ISB30_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP1989640.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4140..8168
                     /gene="rpoB"
                     /locus_tag="ISB30_002009"
     CDS             4140..8168
                     /gene="rpoB"
                     /locus_tag="ISB30_002009"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP1989641.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8245..12468
                     /gene="rpoC"
                     /locus_tag="ISB30_002010"
     CDS             8245..12468
                     /gene="rpoC"
                     /locus_tag="ISB30_002010"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP1989642.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12709..13842)
                     /gene="thiH"
                     /locus_tag="ISB30_002011"
     CDS             complement(12709..13842)
                     /gene="thiH"
                     /locus_tag="ISB30_002011"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAP1989643.1"
                     /translation="MKTFSDRWRQLDWDDIHLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYAELKQLGLDGVMVYQETYHEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(13839..14609)
                     /gene="thiG"
                     /locus_tag="ISB30_002012"
     CDS             complement(13839..14609)
                     /gene="thiG"
                     /locus_tag="ISB30_002012"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAP1989644.1"
                     /translation="MLHIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14611..14811)
                     /gene="thiS"
                     /locus_tag="ISB30_002013"
     CDS             complement(14611..14811)
                     /gene="thiS"
                     /locus_tag="ISB30_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAP1989645.1"
                     /translation="MQILFNDQPMQCVAELTVHELLEQLEQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(14795..15550)
                     /gene="thiF"
                     /locus_tag="ISB30_002014"
     CDS             complement(14795..15550)
                     /gene="thiF"
                     /locus_tag="ISB30_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAP1989646.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIRLMA
                     LQQRLTGETLKDAVAQADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLTLRRASGCPVCGGCNADPV"
     gene            complement(15543..16178)
                     /gene="thiE"
                     /locus_tag="ISB30_002015"
     CDS             complement(15543..16178)
                     /gene="thiE"
                     /locus_tag="ISB30_002015"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAP1989647.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDQR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16178..18073)
                     /gene="thiC"
                     /locus_tag="ISB30_002016"
     CDS             complement(16178..18073)
                     /gene="thiC"
                     /locus_tag="ISB30_002016"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAP1989648.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARKGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEVFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18154..18254)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18306..18782)
                     /gene="rsd"
                     /locus_tag="ISB30_002017"
     CDS             complement(18306..18782)
                     /gene="rsd"
                     /locus_tag="ISB30_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAP1989649.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKYPA"
     gene            18877..19650
                     /gene="nudC"
                     /locus_tag="ISB30_002018"
     CDS             18877..19650
                     /gene="nudC"
                     /locus_tag="ISB30_002018"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAP1989650.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19690..20754
                     /gene="hemE"
                     /locus_tag="ISB30_002019"
     CDS             19690..20754
                     /gene="hemE"
                     /locus_tag="ISB30_002019"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAP1989651.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVASILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20764..21435
                     /gene="nfi"
                     /locus_tag="ISB30_002020"
     CDS             20764..21435
                     /gene="nfi"
                     /locus_tag="ISB30_002020"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAP1989652.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALEWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21478..22068
                     /locus_tag="ISB30_002021"
     CDS             21478..22068
                     /locus_tag="ISB30_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAP1989653.1"
                     /translation="MLQNPIHLRLERLASWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22255..22527
                     /gene="hupA"
                     /locus_tag="ISB30_002022"
     CDS             22255..22527
                     /gene="hupA"
                     /locus_tag="ISB30_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAP1989654.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22540..23235
                     /locus_tag="ISB30_002023"
     CDS             22540..23235
                     /locus_tag="ISB30_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAP1989655.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGQKQQLSNDQIDLYRYRAAQIREISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23237..23656)
                     /gene="zraP"
                     /locus_tag="ISB30_002024"
     CDS             complement(23237..23656)
                     /gene="zraP"
                     /locus_tag="ISB30_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAP1989656.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMGHW"
     gene            23894..25270
                     /gene="zraS"
                     /locus_tag="ISB30_002025"
     CDS             23894..25270
                     /gene="zraS"
                     /locus_tag="ISB30_002025"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAP1989657.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGLHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25267..26592
                     /gene="zraR"
                     /locus_tag="ISB30_002026"
     CDS             25267..26592
                     /gene="zraR"
                     /locus_tag="ISB30_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAP1989658.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26589..27878)
                     /gene="purD"
                     /locus_tag="ISB30_002027"
     CDS             complement(26589..27878)
                     /gene="purD"
                     /locus_tag="ISB30_002027"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP1989659.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27890..29479)
                     /gene="purH"
                     /locus_tag="ISB30_002028"
     CDS             complement(27890..29479)
                     /gene="purH"
                     /locus_tag="ISB30_002028"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP1989660.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKCLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERIPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         6971 a         7731 c         8216 g         6870 t
ORIGIN      
        1 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
       61 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      121 gttgtagcaa aagttctgag ctaattgccg ataacatttg acgcaatgcg cactaaaagg
      181 gcatcatttg atgccctttt tgcacgcttt cgtaccagaa cctggctcat cagtgatttt
      241 ctttgtcata atcattgctg agacaggctc tgttgagggc gtataatccg aaaagctaat
      301 acgcgtttcg atttggtttg cctcgcgatc gcggggtgaa aatgtttgta gaatacttct
      361 gacaggttgg tttatgagtg cgaataccga agctcaagga agcgggcgcg gcctggaagc
      421 gatgaagtgg gtcgttgtgg tggcattgct cctggtagcg attgtcggca actaccttta
      481 tcgcgacatt atgctgccgc tgcgtgcgct ggccgtagta attctgattg ctgcagcggg
      541 tggtgtcgcg ctgttaacga caaaaggtaa agcaaccgtt gcttttgccc gtgaagcgcg
      601 taccgaagtc cgtaaggtca tttggccgac tcgccaggaa acattgcaca ccacgctgat
      661 tgtggctgcg gttaccgcag taatgtcact gatcctgtgg ggactggatg gtattctggt
      721 tcgcctggta tcctttatca ctggcctgag gttctgagat gtctgaagct cctaaaaagc
      781 gctggtacgt cgttcaggcg ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg
      841 agcatatcaa attacacaac atggaagagt tgtttggtga agtcatggta ccaaccgaag
      901 aagtggttga aatccgtggc ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct
      961 acgtcctcgt tcagatggtg atgaacgacg cgagctggca cctggtgcgc agcgtaccgc
     1021 gtgtgatggg cttcatcggc ggtacttccg atcgtcctgc gccaatcagc gataaagaag
     1081 tcgatgcgat tatgaaccgc ctgcagcagg ttggtgataa gccgcgtccg aaaacgttgt
     1141 ttgaaccggg tgaaatggtc cgtgttaatg atggtccgtt cgctgacttc aacggcgttg
     1201 ttgaagaagt tgattacgag aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg
     1261 cgacccctgt agagctggac ttcagccagg ttgaaaaagc ttaacccagc gatcaaaaaa
     1321 gcggcgattt aatcgttgca caaggcgcga gattggaata caatttcgcg ccttttgttt
     1381 ttatgggcct tgcccgtaaa acgatttttt atatcacggg gagcctctca gaggcgttat
     1441 tacccaactt gaggaattta taatggctaa gaaagtacaa gcctatgtca agctgcaggt
     1501 tgcagctggt atggctaacc cgagtccgcc agtaggtccg gctctgggtc agcagggcgt
     1561 aaacatcatg gaattctgca aagcgttcaa cgcaaaaact gattccatcg aaaaaggtct
     1621 gccgattccg gtagtaatca ctgtttacgc tgaccgttct ttcactttcg ttaccaagac
     1681 tccgccggca gcagttctgc tgaaaaaagc ggctggtatc aagtctggtt ccggtaagcc
     1741 gaacaaagac aaagtgggta aaatttcccg cgctcaactg caggaaatcg cgcagaccaa
     1801 agctgccgac atgactggtg ccgacattga agcgatgact cgctccatcg aaggtactgc
     1861 acgttccatg ggcctggtag tggaggacta agaaatggct aaactgacca agcgcatgcg
     1921 tgttatccgc gagaaagttg atgcaaccaa acagtacgac atcaacgaag ctatcgctct
     1981 gctgaaagag ctggcgactg ctaaattcgt agaaagcgtg gacgtagctg ttaacctcgg
     2041 catcgacgct cgtaaatctg accagaacgt acgtggtgca actgtactgc cgcacggtac
     2101 tggccgttcc gttcgcgtag ccgtatttac ccagggtgca aatgctgaag ctgctaaagc
     2161 tgcaggtgct gaactggtag gtatggaaga tctggctgaa cagatcaaga aaggcgaaat
     2221 gaactttgac gttgttattg cttctccgga tgcaatgcgc gttgttggcc agctgggcca
     2281 ggttctgggt ccgcgcggcc tgatgccaaa cccgaaagtg ggtactgtaa cgccgaacgt
     2341 tgctgaagcg gttaaaaacg ctaaagctgg ccagattcgt taccgtaacg acaaaaacgg
     2401 catcatccac accaccatcg gtaaagtaga ctttgacgct gacaaactga aagaaaacct
     2461 ggaagctctg ctggttgcgc tgaaaaaagc aaaaccgact caggcgaaag gcgtgtacat
     2521 caagaaagtt agcatctcta ccaccatggg tgcaggtgtt gcagttgacc aggctggcct
     2581 gagcgcttct gtaaactaat gcctttacgt gggcggtgat tttgtctaca atcttacccc
     2641 cacgtacgct aacgaaagtt agcagctaaa aagatttgtt cgttggagcc tggcctatcc
     2701 aggcctccgt cgaagaccgc aggagtttcg caagaaactt aatcccctgc gtagacggtg
     2761 acagaacgct aagattattc ttttatattc tggcttgttt ctgctcaccg taattaagac
     2821 gctctctccg tttggaggag tgaagtgagt tccagagatt ttctctggca aacatccagg
     2881 agcaaagcta atggctttaa atcttcaaga caaacaagcg attgttgctg aagtcagcga
     2941 agtagccaaa ggcgcgctgt ctgcagtagt tgcggattcc cgtggcgtaa ctgtagataa
     3001 aatgactgaa ctgcgtaaag caggtcgcga agctggcgta tacatgcgtg ttgttcgtaa
     3061 caccctgctg cgccgtgctg ttgaaggtac tccgttcgag tgcctgaaag acgcgtttgt
     3121 tggtccgacc ctgattgcat actctatgga acacccgggc gctgctgctc gtctgttcaa
     3181 agagttcgcg aaagcgaatg caaaatttga ggtcaaagcc gctgcctttg aaggtgagct
     3241 gatcccggcg tctcagatcg accgcctggc aactctgccg acctacgaag aagcaattgc
     3301 acgcctgatg gcaaccatga aagaagcttc ggctggcaaa ctggttcgta ctctggctgc
     3361 tgtacgcgat gcgaaagaag ctgcttaatc gcagttatct ttttaacgca ttcgcttacg
     3421 tataaactta ttctgatatt caggaacaat ttaaatgtct atcactaaag atcaaatcat
     3481 tgaagcagtt gcagctatgt ctgtaatgga cgttgtagaa ctgatctctg caatggaaga
     3541 aaaattcggt gtttccgctg ctgctgctgt agctgtagct gctggcccgg ttgaagctgc
     3601 tgaagaaaaa actgaattcg acgtaattct gaaagctgct ggcgctaaca aagttgctgt
     3661 tatcaaagca gtacgtggcg caactggcct gggtctgaaa gaagctaaag acctggttga
     3721 atctgcaccg gctgctctga aagaaggcgt gagcaaagac gacgcagaag cactgaaaaa
     3781 agctctggaa gaagctggcg ctgaagttga agttaaataa gccaaccctt ccggttgcag
     3841 cctgagaaat caggctgatg gctggtgact ttttagtcac cagccttttt gcgctgtaag
     3901 gcgccagtag cgtttcacac tgtttgacta ctgctgtgcc tttcaatgct tgtttctatc
     3961 gacgacttaa tatactgcga caggacgccc gttctgtgta aatcgcaatg aaatggttta
     4021 agcgtgatag caacaggcat tgcggaaagt gttccatttt ccggtcaaca aaatagtgtt
     4081 gcacaaactg tccgctcaat ggacagatgg gtcgacttgt cagcgagctg aggaacccta
     4141 tggtttactc ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag
     4201 ttctggatgt accttatctc ctttctatcc agcttgactc gtttcagaaa tttatcgagc
     4261 aagatcctga agggcagtat ggtctggaag ctgctttccg ttccgtattc ccgattcaga
     4321 gctacagcgg taattccgag ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg
     4381 acgtccagga atgtcaaatc cgtggcgtga cctattccgc accgctgcgc gttaaactgc
     4441 gtctggtgat ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag
     4501 aagtctacat gggcgaaatt ccgctcatga cggacaacgg tacctttgtt atcaacggta
     4561 ctgagcgtgt tatcgtttcc cagctgcacc gtagtccggg cgtcttcttt gactccgaca
     4621 aaggtaaaac ccactcttcg ggtaaagtgc tgtataacgc gcgcatcatc ccttaccgtg
     4681 gttcctggct ggacttcgaa ttcgatccga aggacaacct gttcgtacgt atcgaccgtc
     4741 gccgtaaact gcctgcgacc atcattctgc gtgccctgaa ctacaccaca gagcagatcc
     4801 tcgacctgtt ctttgaaaaa gttatctttg aaatccgtga taacaagctg cagatggaac
     4861 tggtgccgga acgcctgcgt ggtgaaaccg catctttcga catcgaagct aacggtaaag
     4921 tgtacgtaga aaaaggccgc cgtattactg cgcgccacat tcgccagctg gaaaaagacg
     4981 acgtcaaact gatcgaagtc ccggttgagt acatcgcagg taaagtggtt gctaaagact
     5041 atattgatga gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc
     5101 tgctggctaa gctgagccag tctggtcaca agcgtatcga aacgctgttc accaacgatc
     5161 tggatcacgg cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga
     5221 gcgcactggt agaaatctac cgtatgatgc gccctggcga gccgccgact cgtgaagcag
     5281 ctgaaagcct gtttgagaac ctgttcttct ccgaagaccg ctatgacctg tctgcggttg
     5341 gtcgtatgaa gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtatcctga
     5401 gcaaagacga catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg
     5461 aagtcgatga tatcgaccac ctcggcaacc gtcgtatccg ttctgttggc gaaatggcgg
     5521 aaaaccagtt ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc
     5581 tgggcgatct ggataccctg atgcctcagg atatgatcaa cgccaagccg atttccgcag
     5641 cagtgaaaga gttcttcggt tccagccagc tgtctcagtt tatggaccag aacaacccgc
     5701 tgtctgagat tacgcacaaa cgtcgtatct cggcactcgg cccaggcggt ctgacccgtg
     5761 aacgtgcagg cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa
     5821 tcgaaacccc tgaaggtccg aacatcggtc tgatcaactc cctgtccgtg tacgcacaga
     5881 ctaacgaata cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg
     5941 acgaaattca ctatctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact
     6001 ccaacctgga tgaagaaggc cacttcgtag aagacctggt aacttgccgt agcaaaggcg
     6061 aatccagctt gttcagccgc gaccaggttg actacatgga cgtatccacc cagcaggtgg
     6121 tatccgtcgg tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga
     6181 tgggtgcgaa catgcaacgt caggccgttc cgactctgcg tgctgataag ccgctggttg
     6241 gtacgggtat ggaacgtgct gttgccgttg actctggtgt aactgcggtt gctaaacgtg
     6301 gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga
     6361 tgtatccggg tgaagcaggt atcgacatct acaacctgac caagtacacc cgttctaacc
     6421 agaacacctg tatcaaccag atgccgtgtg tgtctctggg tgaaccggta gaacgtggcg
     6481 acgtgctggc agacggtccg tccaccgacc tcggtgaact ggcgcttggt cagaacatgc
     6541 gcgtagcgtt catgccgtgg aatggttaca acttcgaaga ctccatcctc gtatccgagc
     6601 gtgttgttca ggaagaccgt ttcaccacca tccatattca ggaactggcg tgtgtgtccc
     6661 gtgataccaa gctggggccg gaagagatca ccgctgacat cccgaacgtg ggtgaagctg
     6721 cgctctccaa actggatgaa tccggtatcg tttacattgg tgcggaagtg accggtggcg
     6781 acattctggt tggtaaggta acgccgaaag gtgaaactca gctgacccca gaagaaaaac
     6841 tgctgcgtgc gatcttcggt gagaaagcgt ctgacgttaa agactcttct ctgcgcgtac
     6901 caaacggtgt atccggtacg gttatcgacg ttcaggtctt tactcgcgat ggcgtagaaa
     6961 aagataaacg tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt
     7021 ctgaagaact gcagatcctc gaagcaggtc tgttcagccg tatccgtgct gtgctggtag
     7081 ccggtggcgt tgaagctgag aagctcgaca aactgccgcg cgatcgctgg ctggagctgg
     7141 gcctgaccga cgaagagaaa caaaatcagc tggaacagct ggctgagcag tatgacgaac
     7201 tgaaacacga gttcgagaag aaactcgaag cgaaacgccg caaaatcacc cagggcgacg
     7261 atctggcacc gggcgtgctg aagattgtta aggtatatct ggcggttaaa cgccgtatcc
     7321 agcctggtga caagatggca ggccgtcatg gtaacaaggg tgtaatttct aagatcaacc
     7381 cgatcgaaga tatgccttac gatgaaaacg gtactccggt agacatcgta ctgaacccgc
     7441 tgggcgtacc gtctcgtatg aacattggtc agatcctcga aacccacctg ggtatggctg
     7501 cgaaaggtat cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac
     7561 tgcgcgaatt catccagcgt gcgtacgatc tgggcgctga tgttcgtcag aaagttgacc
     7621 tgagtacctt cagcgatgaa gaagttatgc gtctggctga aaacctgcgc aaaggtatgc
     7681 caatcgcaac gccggtcttc gacggtgcga aagaagcaga aattaaagag ctgctgaaac
     7741 ttggcgacct gccgacttct ggtcagatcc gcctgtacga cggtcgcact ggtgaacagt
     7801 tcgaacgtcc ggtaaccgtt ggttacatgt acatgctgaa actgaaccac ctggtcgacg
     7861 acaagatgca cgcgcgttcc accggttctt acagcctggt tactcagcag ccgctgggtg
     7921 gtaaggcaca gttcggtggt cagcgtttcg gggagatgga agtgtgggcg ctggaagcat
     7981 acggcgcagc atacaccctg caggaaatgc tcaccgttaa gtctgatgac gtgaacggtc
     8041 gtactaagat gtataaaaac atcgtggacg gcaaccatca gatggagccg ggcatgccag
     8101 aatccttcaa cgtattgttg aaagagattc gttcgctggg tatcaacatc gaactggaag
     8161 acgagtaatt ctcgctcaaa caggtcactg ctgtcggggt aaaacccggc agcggattgt
     8221 gctaactccg acgggagcaa atccgtgaaa gatttattaa agtttctgaa agcgcagact
     8281 aaaaccgaag agtttgatgc gatcaaaatt gctctggctt cgccagacat gatccgttca
     8341 tggtctttcg gtgaagttaa aaagccggaa accatcaact accgtacgtt caaaccagaa
     8401 cgtgacggcc ttttctgcgc ccgtatcttt gggccggtaa aagattacga gtgcctgtgc
     8461 ggtaagtaca agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg cgttgaagtg
     8521 acccagacta aagtacgccg tgagcgtatg ggccacatcg aactggcttc cccgactgcg
     8581 cacatctggt tcctgaaatc gctgccgtcc cgtatcggcc tgctgctcga tatgccgctg
     8641 cgcgatatcg aacgcgtact gtacttcgaa tcctatgtgg ttatcgaagg tggtatgacc
     8701 aacctggagc gtcagcagat cctgactgaa gagcagtatc tggacgcgct ggaagagttc
     8761 ggtgacgaat tcgacgcgaa gatgggggcg gaagcaatcc aggccctgct gaagagcatg
     8821 gatctggagc aagagtgcga acagctgcgt gaagagctga acgaaaccaa ctccgaaacc
     8881 aagcgtaaaa agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac
     8941 aaaccagagt ggatgatcct gaccgttctg ccggtactgc cgccagatct gcgtccgctg
     9001 gttccgctgg atggtggtcg tttcgcgact tctgacctga acgatctgta tcgtcgcgtc
     9061 attaaccgta acaaccgtct gaaacgtctg ctggacctgg ctgcgccgga tatcatcgta
     9121 cgtaacgaaa aacgtatgct gcaggaagcg gtagacgccc tgctggataa cggtcgtcgc
     9181 ggtcgtgcaa tcaccggttc taacaagcgt cctctgaaat ctttggccga catgatcaaa
     9241 ggtaaacagg gtcgtttccg tcagaacctg ctcggtaagc gtgttgacta ctccggtcgt
     9301 tctgtaatca ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg
     9361 gcgctggagc tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg tcttgctacc
     9421 accatcaaag ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg
     9481 gacgaagtta tccgcgaaca cccggtactg ctgaaccgtg caccgactct gcaccgtctg
     9541 ggtatccagg catttgaacc ggtactgatc gaaggtaaag ctatccagct gcacccgctg
     9601 gtttgtgcgg catataacgc cgacttcgat ggtgaccaga tggccgttca cgtaccgctg
     9661 acgctggaag cccagctgga agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc
     9721 ccggcgaacg gcgaaccaat catcgttccg tctcaggacg ttgtactggg tctgtactac
     9781 atgacccgcg actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa
     9841 gcagaacgtc tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc
     9901 accgagtatg aaaaagatgc taacggtgaa ttagtggcga aaaccagcct gaaagacacg
     9961 actgttggcc gtgccattct gtggatgatt gtaccgaaag gtctgcctta caccatcgtc
    10021 aaccaggcgc tgggtaaaaa agcaatctcc aaaatgctga acacctgcta ccgcattctc
    10081 ggtctgaaac cgaccgttat ttttgcggac cagatcatgt ataccggctt tgcatatgca
    10141 gcgcgttctg gtgcatctgt tggtatcgat gacatggtca tcccggagaa gaaacacgaa
    10201 atcatctccg aggcagaagc agaagttgct gaaattcagg agcagttcca gtctggtctg
    10261 gtaactgcgg gcgaacgcta caacaaagtt atcgatatct gggctgcggc gaacgatcgt
    10321 gtatccaaag cgatgatgga taacctgcaa actgaaaccg ttattaaccg tgacggtcag
    10381 gaagagaagc aggtttcctt caacagcatc tacatgatgg ccgactccgg tgcgcgtggt
    10441 tctgcggcac agattcgtca gcttgctggt atgcgtggtc tgatggcgaa gccggatggc
    10501 tccatcatcg aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt actccagtac
    10561 ttcatctcca cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa aacggcgaac
    10621 tccggttacc tgactcgtcg tctggttgac gtggcgcagg acctggtggt taccgaagac
    10681 gattgtggta cccatgaagg tatcatgatg actccggtta tcgagggtgg tgacgttaaa
    10741 gagccgctgc gcgatcgcgt attgggtcgt gtaactgctg aagacgttct gaagccgggt
    10801 actgctgata tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg
    10861 gaagagaact ctgtcgacgc ggttaaagta cgttctgttg tatcttgtga caccgacttt
    10921 ggtgtatgtg cgcactgcta cggtcgtgac ctggcgcgtg gccacatcat caataaaggt
    10981 gaagcaatcg gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca gctgaccatg
    11041 cgtacgttcc acatcggtgg tgcggcatct cgtgcggctg ctgaatccag catccaggtg
    11101 aaaaacaaag gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa
    11161 ctggttatca cttcccgtaa caccgaactg aaactgatcg acgaattcgg tcgtaccaaa
    11221 gaaagctaca aagtacctta cggtgcggta ctggcgaaag gcgatggcga acaggttgct
    11281 ggcggcgaaa ccgttgcaaa ctgggacccg cacaccatgc cggttatcac cgaagtaagc
    11341 ggttttgtac gctttactga catgatcgac ggccagacca ttactcgtca gaccgacgaa
    11401 ttgaccggtc tgtcttcgct ggtggttctg gattccgcag aacgtaccgc aggtggtaaa
    11461 gatctgcgtc cggcactgaa aatcgttgat gctcagggta acgacgttct gatcccgggt
    11521 accgatatgc ctgcgcagta cttcctgccg ggtaaagcga ttgttcagct ggaagatggc
    11581 gtacagatca gctctggtga caccctggcg cgtattccgc aggaatccgg cggtaccaag
    11641 gacatcaccg gtggtctgcc gcgcgttgcg gacctgttcg aagcacgtcg tccgaaagag
    11701 ccggcaatcc tggctgaaat cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa
    11761 cgtcgtctgg ttatcacccc ggtagacggt agcgatccgt acgaagagat gattccgaaa
    11821 tggcgtcagc tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac
    11881 ggtccggaag cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt gactcgttac
    11941 atcgttaacg aagtacagga cgtataccgt ctgcagggcg ttaagattaa cgataaacac
    12001 atcgaagtta tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc gggcagctcc
    12061 gacttcctgg aaggtgaaca ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg
    12121 gaagcgaacg gtaaagtggg tgcaacttac tcccgcgatc tgctgggtat taccaaagcg
    12181 tctctggcaa ccgagtcctt catctccgcg gcatcgttcc aggagaccac tcgcgtgctg
    12241 accgaagcag ccgttgcggg caaacgcgac gaactgcgcg gcctgaaaga gaacgttatc
    12301 gtgggtcgtc tgatcccggc aggtaccggt tacgcgtacc accaggatcg tatgcgtcgc
    12361 cgtgctgcgg gtgaagctcc ggctgcaccg caggtgactg cagaagacgc atctgccagc
    12421 ctggcagaac tgctgaacgc aggtctgggc ggttctgata acgagtaatc gttaatccgc
    12481 aaataacgta aaaacccgct tcggcgggtt ttttatgggt gttattcaga gggcaaagtc
    12541 ttcacgggaa atattaagaa aggcagggcg aaacgaggaa gaagcttttc gccctggaca
    12601 acattcctgc tgacggcact accataaaaa aacacaacgc tttcaacaac cttgcgtttt
    12661 tcatcttccg tagcggctcg gaataaattt acgttcttac attctgtctc atggcctttg
    12721 cgatgggcgt cccagatagc tgtcccagtc tttccatacc ggctgcaaac cctgagcggt
    12781 taacgcggca gcaaccgctt ccggtctgcg atcgtcgtgc ggtgagaact gttccagctc
    12841 ggggtggttg tcggcatagc cacctggctg cgttttcgaa aaagcgctga cgttattaat
    12901 tgccagcgga atcacgcgat cgcgaaacca cggtgattcc cgcgtggaga gtgacagttc
    12961 aatctccggt gcaagcagcc ggaaagcgca gatggcttgc actaactggc gttcatccat
    13021 aatcgacgca ggctcaatgc cgccagtaca tggacgcagg cgtgggaagg agacagagta
    13081 gcggctttgc cagtaatgct gttgcagcca tagcaaatgt tcggcaacca tatagcagtc
    13141 aactcgccag ctgtcggaaa ggccgattag cgcgccgagg cctatcttat caatccccgc
    13201 acgccctagc cgatccggcg tttccagccg ccagaagaag tcctgttttt tacctttcag
    13261 atgatgatgg gcataagtcg cctcatgata tgtctcctga taaaccatta ctccatcgag
    13321 gccaagctgt tttaactcgg catattccgc ctccgccagc ggttgcactt ccatctgtag
    13381 tgaagagaac tgttcacgca gggcagggag atgacgacga aagtaatcca tccccacttt
    13441 cgcctgatgt tcaccagtga ctaatagcag atgttcaaag cccatctccc gtatagcggc
    13501 gctttccctg gcaatatccg cttcatccag cgttttgcgc ttgatgcggt tgctcataga
    13561 aaatccgcaa taagtacagt cgttagcgca aagattggaa agataaagcg ggacgtagaa
    13621 gctgacgacg ttgccaaatc gctgacgagt cagacgttgc gctcgctggg ccagtggttc
    13681 cagatagcca ctggcggcgg gtgatagcaa tgccatcatg tcgtcgcggg tgagttgcga
    13741 ggcatttagc gcccgctcta cgtcagcagc cgttttgccg ttgatacgca ggtggatgtc
    13801 atcccagtcc agttgtcgcc agcgatcgct gaaggttttc atgccgatgc ctccagaaat
    13861 ccagtcagcg ggctggtggc atgagcaaaa tgactgcggc tgcccggtcc ggactgacgt
    13921 gccagcaggc ctgcttctac cgccagacga aatgccttcg ccatgttgac gggatcgtcc
    13981 gcgacggcaa tcgccgtatt cactaacacc gcgtcggccc ccatttccag cgcctgcgcg
    14041 gcatggctgg gaacgccgat gccagcatcg acaaccaccg gaacggtggc ctgctggata
    14101 ataatctcca gcatggcgcg ggtttccagt ccctgattcg agccaatcgg cgcgccgagc
    14161 ggcatcaccg ctgcacagcc gacttcttcc agacgtttac acaataccgg atcggccccg
    14221 cagtaaggca gcacgacaaa tccctgttgt accagcgttt cggcggcttt cagggtttcg
    14281 atgggatcgg gcaacagcca gcgggcgtca gggtgaatct ccaattttag ccagtttgtg
    14341 cctaacgctt cacgtgccag atgggcggcg aaaatggctt cttccgcggt tttcgccccg
    14401 gaagtattcg gcagcagggc cacacccgcc gcgataagcg gttcgaggat agcgtcgttg
    14461 tgctggcgca agtcgacacg tttcatcgcc agcgtcacca gttggctgcc ggaagcgcga
    14521 atagactcca ccatcagttg tgaagaggcg aattttccgg tgccagtaaa cagatgtgaa
    14581 tcaaacgttt tgtccgcaat atgtaacatc tcaaccccct gcaataactt gaaaaagcag
    14641 gatctggtcg ccatcctgca cgatatgttg cgcccattgt tcccgcggga cgatttgctg
    14701 attaatcgcc agtgccgcac ctgcttgttg ttgttcgagt tgctccagca gttcgtgaac
    14761 agttagttcg gcgacacact gcatcggttg atcgttaaac aggatctgca ttgcatcctc
    14821 cacataccgg gcaaccgctg gcgcggcgca acgtcagact gcgccactgg ctcgatttac
    14881 cgtcgaacag tcggagttct cccgcaggcg tctctatacc acttaataac ttaatcgctt
    14941 ccagtgcctg taaagtgccc ataaccccga ccaccgggcc aaccacgccc gccgtgcggc
    15001 agttgcgttc tggctcctgg ttatctggcc acaggcagcg gtaacatccc tgctcccagg
    15061 gcggcgtcag taccatcaac tgaccgccaa atccgaccgc gctggcggtg ataagcggcg
    15121 tgttgagtgc cacgcaggtg gcattaatct cctggcgagt cgccatattg tcagtacagt
    15181 cgagcaccac atcggcttgt gcaaccgcat cttttaacgt ctcacccgtt aaccgttgtt
    15241 gtaatgccat cagtcgaata tcgggattca actgtgtcag tcgctgttgg ctgacctgtg
    15301 atttcgggcg atcgatatct tcagtggtaa agaggatttg tcgttgcaga ttgcttaaat
    15361 gcacatcgtc gtcatctgcc agtaccagcg tcccgacgcc agcccccgcc aggtatagcg
    15421 cagcaggtgt acccagcccg ccaagaccaa taatcagcac ctggctgtcg agcagttttt
    15481 gctgcccgtc cagagcgata tcgtcgagca ggatttggcg gctataacgc ataaagtcac
    15541 ggtcattcat cgccaactcc tgcaatttcc agcagctgtg ccgttgccaa acgccagtct
    15601 gcggcttgag taatggcgct gacgacggcg atactgccga cacccgttgc tatcaccgca
    15661 ggcgcgcgcg ccagactgat accgccaatc gccacggtgg gataatccgc caggcgctca
    15721 acatgccgtg ccagctgttc cagtccctgc ggtgcagaag gcatctgttt ggtttgtgtc
    15781 ggaaacacat gtcccagcgc gatataagaa ggacgtgccg ccagcgcgac gtcgatctcc
    15841 atatcgtcat gagtcgaaac gcccagccgc aggcccgctg cacggatggc attgagatcg
    15901 gtggcttgca aatcttcctg ccccagatgg acgccatacg cctgatgctt aatcgccagc
    15961 cgccagtaat cgttgataaa caatcgcgcg ttatagcggc ggcccagcgc aattgccgcc
    16021 accacatcgg cttctacctc ttcatcgcgc tgatctttga tgcgtagctg gagagtacgt
    16081 acgcctgcat ccaacagacg ttcgatccac tgtacgctgt caaccaccgg gtacagtcct
    16141 aaacgaaagg gtacaggagg aaactcaggc tgatacatca cgcttcctcc ttacgcaggt
    16201 agatttctcc gcctctggcg cggaagttct ccgacatatc cgccattccc acttcaatgg
    16261 tttgcgcggc ggcgtaatca cgcacttcct ggctgatttt catcgagcag aatttcggtc
    16321 cacacatgga gcaaaaatgg gcgactttac cggactcttg cggcagggtt tcatcgtgat
    16381 aagcgcgggc agtaaacggg tcgagggcca gattaaactg gtcttcccag cgaaattcga
    16441 agcgggcttt cgacatggcg ttatcgcgaa tttgcgcgcc cggatgccct ttcgccaggt
    16501 cggcggcgtg agcggcaatc ttataggtga taagcccctg tttaacatct tctttatttg
    16561 gcaggcccag atgctctttc ggcgttacgt agcagagcat cgcgcagcca aaccagccaa
    16621 tcatcgccgc gccaatccct gacgtgaagt ggtcatagcc cggcgcgata tcggtagtta
    16681 gcggccccag agtgtaaaac ggcgcttcgt ggcagtgctc taactcctcg gtcatattgc
    16741 ggcgaatcat ctgcatcggc acgtggcctg ggccttcaat catcacctgc acgtcatatt
    16801 cccaggcgat tttggtcagt tcgcccagcg tatgcagctc ggcaaactgt gcttcatcgt
    16861 tggcgtcctg aatagaaccg gggcgcaggc cgtcgcccag cgacagcgaa acgtcataag
    16921 cagcacagat ttcacaaatt tcgcggaagt gttgatagag gaaattttcc tgatgatggg
    16981 agaggcacca tttcgccata atcgaaccgc cgcgagagac gataccggtc aggcgtttcg
    17041 cggtcatcgg cacgtagcgc agcagcaccc ccgcatggat agtgaagtaa tccacacctt
    17101 gctcggcttg ttccagcagg gtgtcgcgga agacttccca ggtaagatct tcagcgatcc
    17161 cgttcacctt ctccagtgcc tggtagatcg gcactgtacc gatcggcacc gggctgttac
    17221 gcaaaatcca ctcgcgggtt tcgtgaatat agcgaccagt ggaaagatcc atcacggtat
    17281 ccgctcccca gcgcgtggac cataccagtt tttccacttc ttcttcgatg gaagaagtga
    17341 ccgccgagtt gccgatattg gcgttaactt ttaccaggaa gttacggcca ataatcatcg
    17401 gctccgattc cggatgatta atgttggccg ggataatcgc acgtccggca gcaacttcat
    17461 cacggacaaa ttccgcagtg atattttccg gcagacgtgc gccaaagctc attcccggat
    17521 gctggtggcg taaaacttcg ctacgtatgc gctcgcgacc catattctcg cgaatggcga
    17581 tgaattccat ttctggcgtg atgataccct tgcgggcgta gtgcagttgg gtgacgcggc
    17641 gtcctgcttt ggcgcgtttt ggcgttaata cgccgctaaa acgcagctcg tcaaggccat
    17701 catctgccag ccgcgcttta gtgtaatcgg aactgcgtac ggtaagttct tcggtatcgc
    17761 cgcgcgcatc gatccacggc tggcgtagtt ttgccagccc ttgctgcacg ttaatggcaa
    17821 tctgcggatc gccatacggg ccggaggtgt cgtagaccgg aatcgcttcg ttttcttcgt
    17881 actgcggctg ttctttgctg ccgccaatta gcgtcgggct aagctggatc tcacgcatcg
    17941 gcacgcgcac gccggggtgt gtgccagtga tgtaaatgcg ttttgagttg ggaaaggcgg
    18001 agccttccag ggtgtcgata aaatgttggg cctgggcgcg ctgttcgcgg cgggtcagtt
    18061 ttgttgcaga catagctcat tccaaaaagt taaggacgtg gcttgtcaga cgacggatga
    18121 agcaagagac gatcgccgca aaggcgatgc gatagcagat taactcttgt tcccttcgca
    18181 ggtattagcc tgatcaggtt ccgcggatcc cgaataaacg gtctcagcca gttaaggcac
    18241 tccgacaaga aattagcccc acaaacgggg cgttgaatgt aaattacgcg ttaacagcgc
    18301 agaactcaag caggatattt gacgcgggcg gcgtcaagca ccagtagaat cagcttatct
    18361 tccagcacaa agcgcgcttc cagtgcctcg ccgatgtcag ataaaacctg ttgaaactca
    18421 aggtaattat catgatcgat ggcggtttcc aggctggaat cgtagtcatc cataatctgt
    18481 tgagtattgg cttcgagttg cggccaaatc ttcgcggcgc gcgcgagttg cccgttgcct
    18541 tccagcttat gaagaatgcg ctcataaata ctgaaatgtc cggcagacaa gtaatcgacc
    18601 aggctctgac aaaaatcatc aagggctttt tcgtttagcc tcatgtacga ttctttgcca
    18661 ggcttaatgc caaccagatt gtagtaagcc acgagcagat gcttacgtac atgtagccag
    18721 cgatcaacca gtttgttact tcctctgacg cgctccgtca ggttatcgag ctggttaagc
    18781 atgattgact ccgcaagttt gtattcaaaa actgctctgt gagaaatgta aaaaccatgt
    18841 taaacatgcc agtgatgcaa aggtagtgca agagctatgg atcgtataat tgaaaaatta
    18901 gatcacggct ggtgggtcgt cagccatgaa caaaaattat ggttgccgaa gggagaattg
    18961 ccatatggcg aagcggcaaa tttcgatctt gtgggtcagc gcgcactaca aatcggcgaa
    19021 tggcaggggg aacctgtttg gttagtacaa cagcagcggc gtcacgatat ggggtcggta
    19081 cgtcaggtca ttgatctcga tgttgggctg tttcaactgg ccggacgtgg cgtgcaactg
    19141 gcggagtttt accgatcgca taaatactgt ggttactgcg ggcatgaaat gtatccaagc
    19201 aaaaccgaat gggcgatgct gtgcagccat tgccgtgagc gttactaccc gcaaatcgcc
    19261 ccctgcatta ttgttgccat ccgtcgcgat gattcgatcc tcctcgccca gcatacccgc
    19321 catcgtaacg gtgtccatac agtacttgcc ggattcgtcg aagtgggtga aaccctcgag
    19381 caggcagtcg cgcgggaagt gatggaagag agcggaatta aagttaaaaa cttgcgttac
    19441 gtgacctccc aaccgtggcc gtttcctcag tcgttaatga ccgcgtttat ggcggaatat
    19501 gacagcggcg agatcgtgat cgacccgaaa gaattgcttg aggcgaactg gtatcgctat
    19561 gacgatttgc cgttactccc gccgcccggc accgtagcgc gccgtctgat agaagatacg
    19621 gtggcgatgt gtcgggcaga gtatgagtga tgatacactg accgcctgac gcactaagga
    19681 acagccaaaa tgaccgaact taaaaacgat cgttatctgc gggcgctgct gcgccagccc
    19741 gttgatgtca ctccagtatg gatgatgcgc caggcgggtc gctatctacc ggaatataaa
    19801 gccacgcgcg cccaggcggg cgattttatg tcgctgtgca aaaatgccga gctggcgtgc
    19861 gaagtgactt tgcaaccgct gcgtcgctat ccgctggatg cggcgatcct cttttccgat
    19921 atcctcaccg tgccagacgc gatgggttta gggctctatt ttgaagccgg agaaggtccg
    19981 cgttttacct cgccagtcac ctgcaaagcc gatgtcgata aactgccaat tccggacccg
    20041 gaagatgagc tgggttacgt gatgaacgcg gtgcgtacca ttcgtcgcga actgaaaggc
    20101 gaagtgccgc tgattggttt ttccggcagc ccgtggacgc tggcgaccta catggtggaa
    20161 ggcggcagca gcaaagcgtt caccgtgatc aaaaaaatga tgtatgccga tccacaggcg
    20221 ctgcacgcgc tactcgataa actggcgaaa agcgtcactt tgtatctgaa tgcgcagatt
    20281 aaagccggtg ctcaggcagt gatgattttc gacacctggg gcggcgtgct taccgggcgc
    20341 gattatcaac agttctcgct ctattacatg cataaaattg ttgatggttt actgcgtgaa
    20401 aacgacggtc gccgcgtacc ggtcacgctg tttaccaaag gtggcggaca gtggctggaa
    20461 gcgatggcag aaaccggttg cgatgcgctg ggcctcgact ggacaacgga catcgccgat
    20521 gcgcgccgtc gcgtgggcaa taaagtcgcg ctgcagggta atatggaccc gtcgatgctg
    20581 tatgcgccgc ctgcccgcat tgaagaagaa gtggcgtcta tacttgccgg tttcggtcac
    20641 ggcgaaggcc atgtctttaa ccttggtcac ggcattcatc aggatgtgcc gccagaacat
    20701 gctggcgtgt ttgtggaggc agtgcatcgc ttgtctgaac agtatcaccg ctaaggagcg
    20761 attatggatc tcgcgtcatt acgcgctcaa caaattgaac tggcttcttc tgtgatccgc
    20821 gaggatcgac tcgataaaga tccgccggat ctgatcgccg gagccgatgt cggatttgag
    20881 cagggcggag aagtgacgcg agcggcgatg gtgctgctga aatacccctc gcttgagctg
    20941 gtggagtata aagtggcccg catcgccacc accatgcctt acattccagg ttttctctcg
    21001 ttccgcgaat atcctgcgct gctggcagcg tgggagatgc tgtcgcaaaa gccggattta
    21061 gtgtttgtcg atggtcatgg gatctcgcat cctcgccgtc ttggcgtagc cagccatttt
    21121 ggcttattgg tggatgtgcc gaccattggc gtggcgaaaa aacggctctg cggtaaattc
    21181 gaaccgctct ccagcgaacc gggcgcgctg gcccctctga tggataaagg cgagcagctg
    21241 gcctgggtct ggcgcagcaa agcgcgctgt aacccgttgt ttatcgctac cggccatcgg
    21301 gtcagcgtgg acagcgcgct ggagtgggta caacgctgca tgaaaggcta tcgtctgccg
    21361 gagccaacgc gctgggcgga cgcggtggcc tcggaacgtc cggcgttcgt gcgctataca
    21421 gcaaatcagc cctaattcag gtaaactgcg gataatttcc gtatttgaga actcaacatg
    21481 ttacaaaacc caattcatct gcgtctggag cgcctggcaa gctggcagca tgtcactttt
    21541 atggcctgct tatgcgaacg catgtacccc aattacgccg tattctgtca gcaaaccggc
    21601 ttcggcgatg ggcagattta ccgccgcatt ctcgatctca tttgggaaac gctgaccgtc
    21661 aaagatgcaa aagtaaattt cgacagccaa ctggagaaat ttgaagaagc gattccttca
    21721 gccgacgatt tcgatctgta cggcgtttat ccggcaatcg atgcttgcgt ggcgttaagt
    21781 gaactggttc attcgcgttt aagtggcgaa acgctcgaac acgcggtgga agtgagtaag
    21841 acctccatca cgaccgttgc gatgctggaa atgactcagg ctggtcgcga aatgagcgat
    21901 gaagagctca aagaaaaccc agctgtagag caagaatggg acattcagtg ggaaatattc
    21961 cgacttttag ctgagtgtga agaacgcgat atcgagctga taaaaggcct tagggcagac
    22021 ctgcgtgagg cgggtgagag caatattggt ataatttttc agcaataaga ccagaaaacg
    22081 tgatttaacg cctgatttgt cgtacctgga gtcttccctt tcgccccccg tctggtctac
    22141 atttgggggg cgaaaaaaag tggctatcgg tgcgtgtatg caggagagtg ctattctggc
    22201 atttccgtcg cactcgatgc ttagcaagcg ataaacacat tgtaaggata acttatgaac
    22261 aagactcaac tgattgatgt aattgcagag aaagcagaac tgtccaaaac ccaggctaaa
    22321 gctgctctgg agtccactct ggctgcaatt actgagtctc tgaaagaagg cgatgctgta
    22381 caactggttg gtttcggtac cttcaaagtg aaccaccgcg ctgagcgtac tggccgcaac
    22441 ccgcagaccg gtaaagaaat caaaattgcc gcagctaacg taccggcatt tgtttctggc
    22501 aaggcactga aagacgcagt taagtaagat tgcgtggcag tgaacagttt taacgaaggg
    22561 gtggtttcac cccttttgtc tttctggcgt cgatcattga tgctggcagg tgcgctgttt
    22621 ctcactgcct gtagtcacaa ctcttcactt cctcccttta ccgccagtgg atttgctgaa
    22681 gaccagggcg cggtacgcat ctggcgaaaa gacagcggcg ataatgtgca tcttcttgcc
    22741 gtgtttagcc cgtggcgcag tggcgatacg acgacgcgag agtatcgctg gcagggcgat
    22801 aacctcacgc ttatcaatat caacgtttac agcaaaccgc cggtaaacat ccgggcgcgt
    22861 tttgacgatc gcggtgactt gagctttatg caacgtgaat ccgacgggca aaagcagcag
    22921 ctttccaatg atcagatcga tttataccgt tatcgtgccg cgcagatccg cgagattagc
    22981 gatgccttac gccaggggag agtcgtgctg cgccaggggc gctggcatgc gatggagcag
    23041 actgtgacca cctgcgaagg gcaaaccatt aaacctgatt tagattcgca ggcgatagcg
    23101 catatcgagc gccgccagag ccgttcttct gttgatgtca gcgtggcatg gctggaagcg
    23161 cccgaaggtt cgcaattact gttggtggca aactctgatt tctgtcgctg gcaacccaac
    23221 gagaaaacgt tctgatttac cagtggccca tacccatatg accgccacca ccgcagccgc
    23281 cgtaacccat tccggtaccg cgcggaatac ccgcttcagc catcgcaata tctcgtttca
    23341 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    23401 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc tttgtcacca
    23461 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    23521 tttgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    23581 caaatgccga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    23641 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttt
    23701 cgtgccaacg gcgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    23761 actcgcctgt tgtgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    23821 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgggca
    23881 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    23941 cgcgatcctc cccgtggtca ttgttgggct ggtggggctg tttgcggtga cagtgattcg
    24001 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24061 tatccgtgct ctcgaatccg gctcgcgcgt cggaatgggg atgcgcatgc atcatgcgca
    24121 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24181 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24241 ttccccgcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    24301 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    24361 tggaatgcac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caatttttat
    24421 cgctttcgac gccagtaaca ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    24481 tatcctcttc gccctggcga cggtcttgct ggcaagcgtg ttgtcattct tctggtatcg
    24541 ccgatatctg cgctcgggcc agctgttgca ggatgaaatg aagcgcaaag agaagctggt
    24601 ggcactgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    24661 taaagggctg gcgaaatact ttgccgaacg cgcgccagca gggggagaag cgcatcaatt
    24721 ggcgcaggtg atggcgaaag aagccgaccg tttaaaccgc gtggtaagcg agctgctgga
    24781 actggttaag ccaacgcatc tggctttgca ggcggtggag ctcaacacgc tgattagcca
    24841 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    24901 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    24961 tctctatctc aacgctattc aggcgattgg tctgcatggc gtgattagcg tgacggccag
    25021 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcgga
    25081 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggactggg
    25141 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg ttgcaagcca
    25201 ggaggggaaa ggcgcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25261 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    25321 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    25381 cgacaggcgc tggagcaggt gcgggaacag gttttcgatc ttgtgctttg cgatgtgcga
    25441 atggcagaga tggacggcat cgccacgcta aaagagatca aagcgttaaa tccggcgatt
    25501 ccggtactga ttatgaccgc gtactccagc gtcgagacgg cggtagaggc actgaaaacc
    25561 ggggcgctgg attacctcat caagccgctg gatttcgata acctgcaggc aacgctggaa
    25621 aaggcgctcg cgcatacgca cagtatcgac gctgaaacgc ctgcggtgtc tgccagccag
    25681 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    25741 gcgccatcgg aagccacggt gctgatccac ggcgattccg gtaccggtaa agagctggtc
    25801 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    25861 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcatgaaaa aggggcgttt
    25921 accggagccg acaaacggcg agaagggcgc tttgttgagg cggacggcgg cacgctgttt
    25981 ctcgatgaga ttggcgatat ctcgccgatg atgcaggttc gtctgctgcg tgcgattcag
    26041 gagcgcgaag tgcaacgtgt cggcagcaac cagactatct cggttgatgt ccggctgatt
    26101 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26161 tactatcggc tgaacgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26221 attcctctgc tggctggcca ttttcttcag cgctttgccg aacgtaatcg caaggcggta
    26281 aaaggcttta cgccccaggc gatggatctg ctgattcatt acgactggcc gggaaatatt
    26341 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    26401 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggtaca aagtcaggat
    26461 attcagccgc tggtggaagt cgaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    26521 ggcaacaaaa ccgaagccgc ccgccagtta gggatcacgc gcaaaacgct attggcaaaa
    26581 ctgtcgcgtt agttctgctc gcgttcgata gcgcgccagc cgatatcttt ccggcagaag
    26641 cagtcgtccc aatggatatc ggtcattaag gcataagcgc gtttctgcgc ttctgccacg
    26701 gtatgaccca gcgcggtaac gcacagtacg cgcccgccgc tggtcactac ctgctcgtca
    26761 tccgccagtt tcgtgcccgc gtggaacact ttgccgtctg ccacttcttc cagcggcagg
    26821 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    26881 ccgagagaag cgcgttcatc ccactcggac gttttctcgt ccagcttgcc ttcacaggcc
    26941 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27001 tcgccaaagc ggcagttaaa ttcaataacc tttggattac cctgtttgtc gatcatcagg
    27061 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27121 cagatgatac gttccatggt gcgctgatga acttcatcgg tcactaccgg cgcaggagag
    27181 taagccccca ttccgccggt gtttggcccg gtatctttat cgcctacgcg tttgtgatcc
    27241 tggctggtgg ccatcggtag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    27301 tcaccgtcga ggaactcttc gataacaata cgatgacccg cgtcgccaaa cgcgttgccc
    27361 gccagcatat cgtgaacagc tgcttcagct tcttccagcg tcatcgccac gataacgcct
    27421 ttcccggctg ccagaccgtc agctttaatg acgattggcg cgcctttctc acgcagatat
    27481 gccagcgcag gttctacctc ggtgaagttc tggtattccg ctgtgggaat gttatggcgg
    27541 gccagaaagt ctttggtgaa tgctttagaa ccttccagtt gtgcagcacc tgcggttggg
    27601 ccgaagattt tcagcccggc ggcgcggaag gtatcgacta cgcctttcac cagcggcgct
    27661 tccgggccga cgatggttag atctaccttt tcgttttgtg cgaaatccag cagcgccggg
    27721 atatcggtta cgccgatagc gacgttctgt agcgcgggtt ccagcgcagt gcctgcattg
    27781 cccggagcaa caaaaacagt ttcaaccagc ggcgactgag ccgctttcca ggccagcgcg
    27841 tgctcgcgcc cgccgttacc gatcactaat actttcatct gtcgctccat taatggcgga
    27901 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaattactt
    27961 cgtcatcacg gatagaaccg ccaggctgga ttacgcaggt cacgcccgca gcggctgcgg
    28021 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc cattgacgaa cctttcactt
    28081 ccaaaccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28141 ggcctgcgcc aataccgatg gtcatattgt ttttggcata gacgatagcg ttggatttca
    28201 cgaacttcgc cactttccag cagaacagcg catcacgaag ttcctgttca gtcggctgac
    28261 gtttggtgac gacgcgcagt tcttccgcac cgaccatccc cagatcgcga tcctgaacca
    28321 gtagaccgcc gttcacgcgt ttgaaatcaa gacccggaat acgttcgccc cactgaccgc
    28381 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    28441 acggcgcaat aatcacttcg acgaactgac gagaaatgat ggcctgcgcg gtttcagcat
    28501 ccagttcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    28561 cgcgatcgta agcatcaaga atggaattgc cgatagccac gccgcaaggg ttggcgtgct
    28621 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    28681 cggcgatgtt gttataagag agagctttac cctgaacctg ggttgcggta gcaacggagg
    28741 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    28801 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    28861 ctttgctttc accgtggtaa gccggaacca tgctgccgaa gtagttggca atcatgctgt
    28921 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    28981 gcgatccttc gttggcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29041 cgattgcgac atctttatgg ttcttggcgg cggagcgcac catcgttggg ccgccgatat
    29101 cgatgttctc taccgcatct tccagcgagc agccttcacg agcgacggtc tgggcgaacg
    29161 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29221 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    29281 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacatt
    29341 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    29401 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    29461 ctggacgacg ttgttgcatg gtaaatcccc tggatttgac tattacagag agcgttagct
    29521 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    29581 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    29641 ttttgacagt ggctaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    29701 tgtgtgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    29761 tgcaattttt ctattgcggc ctgcggag
//
LOCUS       DACADF010000048        29765 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.048, whole genome shotgun sequence.
ACCESSION   DACADF010000048 DACADF010000000
VERSION     DACADF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.048"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..528
                     /locus_tag="ISB30_002029"
     CDS             <1..528
                     /locus_tag="ISB30_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HAP1989661.1"
                     /translation="QISAASNCWNNHVGIIIGHNGEDFLVAESRVPLSTITTLSRFIK
                     RSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLRKLYHTGFKYESSRQFCSKFVFDTYK
                     EALCIPVGEIETFGELLNSNPNAKLTFWKFWFLGSIPWERKTVTPASLWHHPGLVLIH
                     AVGVETPQPELTEAV"
     gene            complement(530..1051)
                     /gene="ruvC"
                     /locus_tag="ISB30_002030"
     CDS             complement(530..1051)
                     /gene="ruvC"
                     /locus_tag="ISB30_002030"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAP1989662.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1086..1826)
                     /locus_tag="ISB30_002031"
     CDS             complement(1086..1826)
                     /locus_tag="ISB30_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP1989663.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1855..2307)
                     /gene="nudB"
                     /locus_tag="ISB30_002032"
     CDS             complement(1855..2307)
                     /gene="nudB"
                     /locus_tag="ISB30_002032"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAP1989664.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2300..4072)
                     /gene="aspS"
                     /locus_tag="ISB30_002033"
     CDS             complement(2300..4072)
                     /gene="aspS"
                     /locus_tag="ISB30_002033"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP1989665.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGSLRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4382..4948
                     /locus_tag="ISB30_002034"
     CDS             4382..4948
                     /locus_tag="ISB30_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP1989666.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPVALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            4945..5763
                     /locus_tag="ISB30_002035"
     CDS             4945..5763
                     /locus_tag="ISB30_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP1989667.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRYAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5816..6211
                     /locus_tag="ISB30_002036"
     CDS             5816..6211
                     /locus_tag="ISB30_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989668.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6252..6995
                     /gene="cmoA"
                     /locus_tag="ISB30_002037"
     CDS             6252..6995
                     /gene="cmoA"
                     /locus_tag="ISB30_002037"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAP1989669.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            6992..7963
                     /gene="cmoB"
                     /locus_tag="ISB30_002038"
     CDS             6992..7963
                     /gene="cmoB"
                     /locus_tag="ISB30_002038"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAP1989670.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPTQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(7999..10428)
                     /locus_tag="ISB30_002039"
     CDS             complement(7999..10428)
                     /locus_tag="ISB30_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAP1989671.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDHIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKNDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYIRYGDFRADPVK
                     NALGTPSGKIEIYSRTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNALK
                     LTAFDQPAIQA"
     gene            complement(10453..11553)
                     /locus_tag="ISB30_002040"
     CDS             complement(10453..11553)
                     /locus_tag="ISB30_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAP1989672.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDISSQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGDLATVTPGTD
                     LTVVDVSGKQPIVRLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDSALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(11941..12687)
                     /gene="cutC"
                     /locus_tag="ISB30_002041"
     CDS             complement(11941..12687)
                     /gene="cutC"
                     /locus_tag="ISB30_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP1989673.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLTELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(12701..13267)
                     /locus_tag="ISB30_002042"
     CDS             complement(12701..13267)
                     /locus_tag="ISB30_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP1989674.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPLQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            13483..15216
                     /gene="argS"
                     /locus_tag="ISB30_002043"
     CDS             13483..15216
                     /gene="argS"
                     /locus_tag="ISB30_002043"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP1989675.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(15269..15661)
                     /locus_tag="ISB30_002044"
     CDS             complement(15269..15661)
                     /locus_tag="ISB30_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HAP1989676.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTMAFSGIPAAEPL
                     RFIWEVPGGGRLTPPLKIQRNEVIVNYR"
     gene            complement(15661..17739)
                     /gene="flhA"
                     /locus_tag="ISB30_002045"
     CDS             complement(15661..17739)
                     /gene="flhA"
                     /locus_tag="ISB30_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAP1989677.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNSVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEMGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(17732..18880)
                     /gene="flhB"
                     /locus_tag="ISB30_002046"
     CDS             complement(17732..18880)
                     /gene="flhB"
                     /locus_tag="ISB30_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAP1989678.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRTFSAQTGAELLKAILKTILVGSVTG
                     FFLWYHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(19069..19713)
                     /gene="cheZ"
                     /locus_tag="ISB30_002047"
     CDS             complement(19069..19713)
                     /gene="cheZ"
                     /locus_tag="ISB30_002047"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HAP1989679.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVIDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQGQVDDLLDSLGF"
     gene            complement(19724..20113)
                     /gene="cheY"
                     /locus_tag="ISB30_002048"
     CDS             complement(19724..20113)
                     /gene="cheY"
                     /locus_tag="ISB30_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HAP1989680.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(20128..21177)
                     /locus_tag="ISB30_002049"
     CDS             complement(20128..21177)
                     /locus_tag="ISB30_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HAP1989681.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(21180..22040)
                     /gene="cheR"
                     /locus_tag="ISB30_002050"
     CDS             complement(21180..22040)
                     /gene="cheR"
                     /locus_tag="ISB30_002050"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAP1989682.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRALGLADFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVSLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(22059..22285)
                     /locus_tag="ISB30_002051"
                     /pseudo
     CDS             complement(22059..22285)
                     /locus_tag="ISB30_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22331..23992)
                     /gene="tar"
                     /locus_tag="ISB30_002052"
     CDS             complement(22331..23992)
                     /gene="tar"
                     /locus_tag="ISB30_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HAP1989683.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQSELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLAAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLAATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(24137..24640)
                     /gene="cheW"
                     /locus_tag="ISB30_002053"
     CDS             complement(24137..24640)
                     /gene="cheW"
                     /locus_tag="ISB30_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HAP1989684.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(24661..26625)
                     /gene="cheA"
                     /locus_tag="ISB30_002054"
     CDS             complement(24661..26625)
                     /gene="cheA"
                     /locus_tag="ISB30_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAP1989685.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVDVSPKISTSPMLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSMQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(26630..27556)
                     /gene="motB"
                     /locus_tag="ISB30_002055"
     CDS             complement(26630..27556)
                     /gene="motB"
                     /locus_tag="ISB30_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HAP1989686.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKRPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(27553..28440)
                     /gene="motA"
                     /locus_tag="ISB30_002056"
     CDS             complement(27553..28440)
                     /gene="motA"
                     /locus_tag="ISB30_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAP1989687.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(28567..29145)
                     /gene="flhC"
                     /locus_tag="ISB30_002057"
     CDS             complement(28567..29145)
                     /gene="flhC"
                     /locus_tag="ISB30_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HAP1989688.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(29148..29498)
                     /gene="flhD"
                     /locus_tag="ISB30_002058"
     CDS             complement(29148..29498)
                     /gene="flhD"
                     /locus_tag="ISB30_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HAP1989689.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         6999 a         8101 c         7296 g         7369 t
ORIGIN      
        1 caaatatcag ctgcatcaaa ttgctggaat aatcacgtcg ggatcattat cggtcataac
       61 ggtgaagact ttctggttgc agaaagccgt gttcccctct caaccatcac tacgctatcc
      121 cgttttatta aacgctctgc taatcaacgc tatgctataa agcgattaga cgccggacta
      181 acagaacaac aaaaacaacg aattgttgaa caggttcctt cccggctacg caaactttac
      241 cacaccggtt ttaaatacga atcttcgcga cagttctgtt caaaatttgt ttttgatact
      301 tataaagagg cgctatgtat tccggtgggt gaaatagaga cgtttggaga attattaaat
      361 agcaatccga atgcaaaact cactttctgg aaattctggt tcttaggttc tattccgtgg
      421 gagcgtaaaa ccgtcacgcc agccagtttg tggcatcatc cgggtttggt gttgattcac
      481 gcggtgggag ttgaaacgcc tcagcctgaa ctgaccgagg cggtataact taacgcagtc
      541 gccctctcgc caggttcagt cgcgattcac tcatttgcat cgcattctga ctaacgtggc
      601 agtgggtgat ggcaatcgcc agcgcatcgg cggcatccgc ctgtggatta gcgggcagtt
      661 tcagcaaggt gcggaccata tgctgcacct ggcttttttc ggcactacca atacctacca
      721 ctgtttgctt tacctgacgt gccgcatatt caaataccgg caattcctga ttcaccgccg
      781 ccacaatcgc cacgccgcgc gcctgcccca gtttcagggc tgagtcagcg ttcttcgcca
      841 taaagacttg ttcaatggcg aaataatcag gctggaattg ggtgatgatt tccgtcacgc
      901 ccgcatagat gagcttcaga cgagacggta aatcatccac tttggtgcgt atgcatccgc
      961 tacccaggta ggacagttgc ctgcctacct ggcggatgac gccatagccg gtcacgcgcg
     1021 aacccgggtc aataccgaga ataatagcca tcacgcgtct ccgttttgct gtttagcagg
     1081 cctcatcaga gagtcgctgc aacctcatca gagatttcac cgttatggta aacttcctgc
     1141 acgtcgtcgc aatcttccag catatcgatc agacgcatca gtttcggtgc ggtttctgca
     1201 tccatatcag ctttggtgga cgggatcatg gaaacttccg cgctgtctgc tttcagacct
     1261 gccgcttcca gagcgtcgcg tactttgccc atttcttccc atgcagtgta gacatcaatc
     1321 gcgccgtcat cataggtcac aacgtcttca gcaccggctt ccagggctgc ttccatgatg
     1381 gtgtcttcat cgcctttctc gaaggagatc acgccttttt tgctgaacag ataagctacg
     1441 gaaccatcag taccgaggtt accgccacat ttgctgaatg catgacgcac ttcagcaacg
     1501 gtacggttgc ggttgtcaga cagacattca atcatgattg ccgtgccgcc aggaccgtaa
     1561 ccttcgtaga tgatggtttc catgtttgca tcatcatcac cgcccacacc tcgtgcaatt
     1621 gcgcggttca gagtgtcacg ggtcatgttg ttagacagtg ctttatcaat tgctgcacgc
     1681 agacgcggat tagcgtccgg atcaccaccg cccagcttag ccgcggttac cagctcacga
     1741 atgattttag tgaagatttt accgcgctta gcatcctgcg cagctttacg atgtctggtg
     1801 ttggcccatt tactatgacc tgccataaaa atatctccag atagccctgc ctgttcaggc
     1861 agcgttaatt acaaactgtt caatcgcctg ccggttgctc caggacttag tgagcgccgc
     1921 cgcagcaggc gcatcaagcc acttgtaagc cagatgttca gtgaaaacga tctggcgctc
     1981 gtgcgggagc gcaagacaga accatgattc cgtattacgc gtcacgcccg gcgcatagcg
     2041 atgacgtaaa tgtgaaaaaa tttcaaactc taccgtgcgc tgacagtcaa ttaaggtcag
     2101 ttgttcagcg acaacatcaa tggtgacctc ttcctttact tcgcgcatgg cagcttgcgg
     2161 cgcggtttca ccctcttcca cgctgccggt taccgactgc cagaaatcgg gatcgtcacg
     2221 ccgctgcaac atcagcaccc gtttcgtatc ttgtgcgtag atgaccacta agatcgaaac
     2281 gggacgctta taagccatat cagttattct cagccttctt cacaacctga atgctcagct
     2341 cagccagtgc agtcgggtta gcaaagctcg gtgcttcagt catcagacac gccgccgccg
     2401 tggttttcgg gaaggcgata acgtcacgga tattgtcggt gccggtcagc agcatggtca
     2461 gacggtcaag accgaatgcc agacctgcgt gcggcggagt accgtatttc agggcgtcga
     2521 gcaggaagcc gaatttctcg cgctgttcct cttcgttgat acccagaata ccaaacaccg
     2581 tctgctgcat atcaccatta tggatacgca cagaaccacc gcccacttcg taaccattga
     2641 tgaccatatc gtaagcgtta gccaccgcat tttccggtgc agctttcagt tctgctgccg
     2701 tcatgtcttt cggtgaggtg aacggatggt gcattgctgt caggccgcct tcaccgtcgt
     2761 cttcaaacat cgggaagtcg ataacccaca gcggtgccca tttgctttcg tcggtcagac
     2821 caaggtcttt acccactttc aggcgcagtg agcccatcgc gtcggcaaca attttcttgt
     2881 tgtcggcacc gaagaaaatc atatcgccat cttgcgcgcc agtacgctcc aggatggctt
     2941 cgatgatttc tgcattaagg aacttcgcta ccgggctgtt gataccttcc agacctttcg
     3001 cgcgttcgtt aactttgatg taagccagac ctttcgcgcc gtagatttta acgaagttac
     3061 cgtattcgtc gatctgctta cgggtcaacg atgcgccgcc cggaacacgc agagcggcaa
     3121 cacggccttt cggatcgttc gccggacctg caaatactgc aaactcaaca gatttcagca
     3181 gatcggcaac gtcggtcagt tccatcgggt tacgtagatc tggtttatca gaaccataac
     3241 ggcgttctgc ttctgcaaag gtcattaccg ggaaatcgcc cagatccacg cccttcactt
     3301 ccagccacag atgacgcacc agcgcttcca tcacttcacg cacttgcggc gcggtcatga
     3361 aagaagtttc cacatcgatc tgagtaaatt caggctgacg gtcagcacgc aggtcttcgt
     3421 cacggaagca tttaacgatc tgatagtagc ggtcaaagcc ggacatcatc agcagctgtt
     3481 tgaacaactg tggggattgc ggcagtgcgt agaatttacc tttgtgcaca cgagaaggca
     3541 ccaggtagtc acgcgcgcct tccggcgtgg ctttggtcag catcggagtt tcgatgtcga
     3601 ggaagccgtg gtcatccata aaacggcgca ccaggctggt gattttagcg cgggttttca
     3661 ggcgctgagc catttccggg cgacgcaggt cgaggtagcg gtatttcaga cgcgcttctt
     3721 cggtgttgac gtggttagag tcaagcggca gaacatctgc acggttgatg atagtcagcg
     3781 aggacgccag tacttcgatt tcgccagtcg ccatatcgcg gttaatattt ttttcgtcac
     3841 gcgcacgtac ggtgcccgtg acctgaatgc agaactcatt acgcagttca gaggccagct
     3901 ttaacgcgtc cgcacgatcc ggatcgaaaa atacctgcac gataccttcg cggtcgcgca
     3961 tatcgatgaa gatcaggcta ccaagatcac gacgacggtt gacccaacca cacagagtca
     4021 cctgctgccc cacgtgggac aaacggagct gtccacaata ttctgtacgc atgagatatc
     4081 ccttaactta gctgccggcg gatgccccct gctgcgcagg tgaccaagtc gcagcgttag
     4141 ctgtatgtca caactgaatg aaaaaaggcg gctattatac tggaaattct gccgcaccgt
     4201 aagagcctgg cccgcgctgg aacgcctcgt taccacttta tatcgggcct gaaatcagac
     4261 tctacgccag tttgctataa aggtgttgcc cgaactcata aaaattaaca aaatttgtcg
     4321 ttccgccatc ggctcatcgt attaaggtga gaggcacgat tttgttttat caggagtcat
     4381 catgcttgaa cttaatgcta aaaccaccgc gctggtggtg attgatttac aagaaggcat
     4441 cttgcctttt gccggtggtc cacatactgc cgatgaggtg gttaatcgcg ccgggaagct
     4501 ggcggcgaaa tttcgcgcga gcggtcagcc cgtgtttctg gtgcgtgttg gctggtctgc
     4561 cgattatgct gaagcattaa aacagccggt tgatgcccct tcccccgcta aagtgttgcc
     4621 cgaaaattgg tggcaacatc ctgtagcatt aggtgcaacc gacagcgata tcgaaatcat
     4681 caaacgtcaa tggggtgcgt tttacggtac ggatctggaa ttgcagttgc gccgccgggg
     4741 tatcgataca atagtgttat gtgggatctc gaccaatatc ggtgttgaat ccaccgcccg
     4801 caatgcctgg gaactcggtt ttaatctggt gattgccgaa gatgcctgta gcgccgctag
     4861 cgccgagcag cacaataaca gcattaatca tatctacccg cgcatcgccc gtgtgcgtag
     4921 cgttgaagag atcctccacg cgttatgatt tacatcggtt tgccacaatg gtcgcatcct
     4981 aaatgggtgc ggttggggat caccagcctt gaagagtatg cccgccactt taactgtgtg
     5041 gagggtaaca ccacgcttta tgccctgccg aaacccgagg ttgtcctgcg ctggcgtgag
     5101 cagaccacag atgacttccg cttctgtttt aagtttccgg cgactatttc gcatcaggca
     5161 gcattacggc attgcgatga tttagtgact gaatttttga cccgcatgtc accgttggct
     5221 ccgcgcattg gacaatactg gctgcaactg cctgccacat tcggcccacg ggagctgcct
     5281 gcgctttggc attttctcga ttctcttccc ggcgaattta attatggtgt ggaagtccgc
     5341 catccacagt ttttcgccaa aggggaagag gaacaaacgc ttaatcgcgg tttacatcag
     5401 cgcggcgtta atcgggtgat tttagacagc cgcccggttc atgcagcacg tccacacagt
     5461 gaagctattc gctacgctca acgaaaaaaa cctaaagttc cggtacatgc tgtactgacg
     5521 gcgaaaaatc cactgatccg ttttatcggt agtgatgata tgacgcaaaa ccgggaatta
     5581 tttcaggtct ggttacaaaa attagcgcag tggcatcaga ccactacgcc ttatcttttt
     5641 ttacatacgc cagatattgc ccaggccccg gaactggtac ataccctgtg ggaagactta
     5701 cgtaaaacgc ttccagagat cggagcagtt ccggctattc cacagcaatc ttctcttttc
     5761 tgaatttgcc acctatcata gacaggtgcc atcggccatt ttaaagggag tttgtatggt
     5821 aagcgcgctg tatgccgttt taagtgcgtt gttattaatg aagttctctt ttgatgtcgt
     5881 tcgcctgcga atgcagtacc gcgttgccta tggcgacggc ggttttagcg aactgcaaag
     5941 cgctattcgc attcatggta acgcggtgga atatattccc atcgcgattg tgttgatgct
     6001 gtttatggaa atgaatggcg cagaaacctg gatggtgcat atttgcggca tcgttttgct
     6061 tgctggtcgt ctgatgcatt attacggttt tcatcaccgt ctgttccgct ggcgacgttc
     6121 tggcatgagc gccacctggt gtgcgctgtt gctgatggtg ctggcgaatc tttggtatat
     6181 gccctgggag ttggttttct ccctgcgtta gcgcacaata cgccactttc tttttcccgg
     6241 atttttacgt tatgtctcac cgcgacacgc tattttctgc ccctatcgcc agactgggcg
     6301 actggacctt tgatgaacgg gtagctgaag tcttcccgga tatgatccag cgttccgttc
     6361 ccggctattc caatattatt tccatgattg gtatgttagc cgagcgcttc gttcaacctg
     6421 gtacgcaggt ttacgatctg ggttgttctc tgggcgcggc gacactctcg gtgcgtcgca
     6481 acattcatca tgataattgc aaaattattg ccatcgataa ctccccggcg atgattgaac
     6541 gctgccgtcg tcatattgac gcctataaag ctcctacgcc agtagacgtt attgaaggtg
     6601 atattcgcga tatcgccatt gaaaacgcat cgatggtggt gctgaatttt accctgcaat
     6661 tcctggaacc ttccgagcgc caggcgttac tggataaaat ttatcaagga ctgaacccgg
     6721 gcggcgcgct ggtgctttcg gaaaaattca gtttcgaaga tgccaaagtt ggtgaactgc
     6781 tgttcaacat gcaccacgac tttaaacgtg ccaacggtta cagcgaactg gagatcagcc
     6841 agaaacgcag catgctggaa aacgtgatgc tgaccgattc cgtggaaacc cataaagcac
     6901 gcctgcataa agccggtttt gagcatagcg agctgtggtt ccagtgcttt aactttggtt
     6961 cactggtggc attaaaagca gaggacgctg catgatcgac tttggtaact tttattctct
     7021 gattgccaaa aatcatcttt cacactggct cgaaacgctg cccacgcaga ttgctaactg
     7081 gcagcgcgag cagcagcacg ggctgtttaa gcagtggtcc aacgcggtgg aatttctgcc
     7141 tgaaattaaa ccgtatcgtc tggatttatt gcatagcgta accgccgaaa gcgaagagcc
     7201 actgagcgcc gggcaaatta agcgcattga aacgctgatg cgcaacctga tgccgtggcg
     7261 caaagggccg ttctcactgt atggcgtcaa catcgatacc gaatggcgtt ccgactggaa
     7321 atgggatcgc gttctgcccc atctttctga tttaaccggg cgcaccattc ttgatgtcgg
     7381 ctgcggcagc ggttatcaca tgtggcgcat gattggcgct ggcgcgcacc tcgcggtggg
     7441 gatcgaccct acacaactgt ttctgtgcca gtttgaagcg gtgcgtaaac tactgggtaa
     7501 cgatcagcgc gcacatttgt taccgttagg tattgaacaa cttccggcac tgaaagcctt
     7561 tgataccgtc ttttcgatgg gcgtgcttta tcatcgtcgt tcaccgctgg agcatctctg
     7621 gcagttaaaa gaccaactgg tgaatgaagg cgaactggtg ctggaaacgc tggttattga
     7681 tggcgatgag aacacggtgc tggtgccggg cgatcgttac gcacaaatgc gtaatgtcta
     7741 tttcattcct tccgcgctgg cgctgaaaaa ctggctgaag aagtgtggtt ttgttgatat
     7801 tcgcattgcg gatgtgagcg ttaccaccac agaagagcag cgacgcaccg aatggatggt
     7861 caccgagtct ctggccgatt ttctcgaccc gcatgatccg ggtaaaacgg tggaaggtta
     7921 tcctgcgcct aaacgcgcgg tgctgattgc gcgcaagccg taaaaattta gcgcccgttt
     7981 ccgggcgctt tttacgtctt atgcctgaat agctggctga tcaaacgccg ttaacttcag
     8041 cgcattgccc gtatattttt cgatatacac cagcgcagag ttaccggcac aggcattcgc
     8101 cagctgcgag ctggggatat ccgccgttaa cacgttcgca ctaccgtttt tacacaagcc
     8161 attttccaga tctggccatg caccttcatg cacgcatacc acgccttttt tgatcccgtc
     8221 agtcaccacc gcgcctgtca gaatctgacc acgtttgttc cagacacgca ccagatcgcc
     8281 attcgcaata ccaaagcgag cagcatcttc ggtgtgaata gtgattggtt cacgatcggc
     8341 aaccgcatat tttttacgca gttccgcata gttaagctga ctgtgtaaac ggtgtgccgg
     8401 atgtgcggtc agaagctgca actgcttctc gtcggcggta cccttccact catcaggcgc
     8461 aagccaggtt gggtgtgccg ggcaatcctt atagccaaat ttttccagcg ttctggagta
     8521 aatctcaatt ttgccgcttg gcgtacccag cgcatttttc accggatcgg cgcggaaatc
     8581 accataacga atgtactgtt cgttcttctc gctgcggcgc atttcgatca gtttattttg
     8641 ctgccagaag gcattaaaca tcggcatcgt cacgcgttgc gcacgggcac ctttctgagc
     8701 ggcatcatag aaaaatttca gccacgccat ttcatcttta ccttcggtat agatctcttt
     8761 tccgccaggt ttgagtaatt cagcaagatc ggcaaacacg tcaaaatcgt tgcgcgcttc
     8821 aaattgcgga gcgacagcct gcttcatcgg cacaatatgc tggttgctgt aatcaccggt
     8881 catcgtcagg tcattgcgct caaacgatgt ggtgatcggt aatacgatat ctgcgtgttt
     8941 ggctgctgcg gtccagtagc attcagaaac gacgatcatc tccggtttct gccatgcttt
     9001 aatcagacga ttagtatcct ggtgatgggt aaagttaccg ccacccgccc accagatcat
     9061 tttgatattc ggatacgttt gttctttacc gttatgctga tatttgccgc caggattttc
     9121 cagcgcatcg acgatacggg caacaggaat cgccgtcatt ccgccatcat cagcggcctc
     9181 gctggcctgt ccggcaatcg ccgcagacat ttctggtagc acgccgccaa cacgcgtcgg
     9241 gttgccaccg ttggagtaat gataagagaa accaaacccg ccgcccggtg taccaatttg
     9301 ccctaacatc gccgccagcg tcaccagcat ccagtgtttc tgttcaccgt attgctggcg
     9361 ctgaattccc cagcctgcca tcagcattgt gcgatttgcc gccatcagtt ctgcaagttt
     9421 cacaatctgc gcttccggca cgccagtaat ttccgccgcc caggccgcac tcttcggcgt
     9481 attatcgttc ttgcctgtca gatactcttc aaactgcgga tacccggtag tgtatttttc
     9541 aaggaacact ttatcgtgtt tgccttgtgt catcagggta tgcgcaatcc ctaacatcag
     9601 tgccacgtcg gtgcccatat tcggtgcgat ccaggtggca ttatcgccaa agaattcgat
     9661 ggtttcggag cggataggat caatggcaat cactggtttg ccagattttt tcagctgatg
     9721 gaagtattcc agcccttgtt catcggtact gctccaggca atttttaagg tattcagcgg
     9781 gttcatgccc cacaacacca ccacctgact gttttccaga atcagcggcc aggaagtctg
     9841 ctgttcatag acctccacag aaccgaccac atgcggcatg atcacctgtg ccgctccggt
     9901 tgaataatcg ccgctatgcc cggaataacc gcccgccagg ttcatataac gttgcagtaa
     9961 ggtttgcgct ttatgcaaca cgccagaaga acgccagccg taagatccgg caaaaatggc
    10021 cgatggtccg ttagctttac gaatatgatc gtgttgctca tgaatcagtt ttaatgcttg
    10081 ttcccagctc acctgtacat aggtatcttc gccacgacct ttcgccggtt gcagtggatt
    10141 atcgagatag ctttttctca ccatcggatg ctgaatacgc gccgtggtgt gtacctgatc
    10201 cgccgccgta gactgtaagg agttcggtat ggtcttcgcc agcgcgcctg tcgaagaaac
    10261 aatcttgccg tccttcactt ctacgttcat cgctccccaa cgtcccgcgg tgagaatttt
    10321 gccgcccttc tcttctgccc atgcaggtaa cggtgcagcc gatgtcacca ccagcgcccc
    10381 agcggcaata ccgctatgtt taataaattc gcgtcttgtt aatgtcataa cttcctccct
    10441 gatcaacgag gatcactgtt tctcggtaat atctttggcg ttgtactgga agtaccgcgt
    10501 caaaatgtcc agttcgtttt cgctcatgct ggttcgcgca cccattcctt tggcaatgga
    10561 cggccatgca ttgacggtgt aatggtcggc ggcaataggg gcatggcagc cagcgcaata
    10621 ggtatcgtca agtttttcag cgtattgcca tagtggttta cggtccgcta atgcggaatc
    10681 ggttagcgca ccctgtaaag acgcctgacg ccattgattg ccgtattcgt cagcctgcca
    10741 ttccccgttt accgttagcg ccttgatacc ttcttcactt aatgtggcga gcgccagccg
    10801 ttgacctgcc gccaggtaga gcgtgttttc actgccctgc atttgataac cctgcaaccg
    10861 aacaattggc tgtttaccac tgacatcaac gacggtgaga tcggtaccag gagtcacggt
    10921 agccaggtcg cctatgcgag aagttttgaa aggataaata tgtgcgccat tagtaactga
    10981 agtagcggcc tgactttcca gctcatgcgc cgcgttgtca tccattttta tttctggcgg
    11041 gaaatgggca atgcctttat ggcaatcgat acaagtttcg ccgtcctttt gcgctttatt
    11101 atgcattttc tgcgcagatt cactttgcga ggaaatatcc atggcatcaa aagaatggca
    11161 actacggcag gttgcagagt cagttgcttt taattctttc catactgttt cggccatttc
    11221 ctggcgatga gcttcgaact tatcgtcact gtctattttg ccgctaacaa attcatgata
    11281 aatatcttta gatgctttta atttagcaaa taaataatcc atccctgact ttggaatatg
    11341 gcaatcggca cattccgcgc gtataccttt ttggttcgaa aagtggacag ttccctgata
    11401 ttcctcaaaa ggtttactca tcgagtggca ggaaaggcaa aatgcagtat ccgacgtttt
    11461 atgtaaggct ttttgcgcca gcaataaccc gccgccacca accacaaccg ctatcagcaa
    11521 aaacaataac ccaatgcgtt ttttccctcg catagattcc tctaattaaa ttccaggaca
    11581 ccgataccat tgtataaata aatatacaac gaattgacta cttgcttaat tcggcacaac
    11641 actgcctgaa ataatcgata aagaatatga tttattacaa tgtaatcatt aattgctaag
    11701 gaataaccca gttgttattt agaatatata tttgtcagtt ttggcaattt aaatcacata
    11761 atgaatgtga atatatagaa tatttctagc acaatgctat gcgcactgtc acccgctcaa
    11821 cagatcacgg caatcgttcg ttttttatac tactcaggga taaaaaaagg cccctgttga
    11881 aattgcaggg gcctggtacg agcaagcatc atattgggcg acatgatgca acggtaaaaa
    11941 tcatttggcc tgatggcgtt caatgattcc tttcatttca gcaaccgccg ccccgtctac
    12001 gacataacgc gaatactcgt ccgcgtgtgc atctgatgac atggacaatc cttgattacg
    12061 ataacgcatc ggtgaggctt gccacgctcc cgcggagcta tggacttcca gcactccggc
    12121 atcgaggaag tggtgcaggt tttccgcacg gactcctgct ccggccataa tgattggagc
    12181 atcacgatgg gcaataagtt ccataatttt tgataaacct tgcagtgcgt ctgatttttg
    12241 ccctgatgtc agtacccggg caatgcccaa ttccgtaaga ttattgagtg tatttaaagg
    12301 gttagcgcac atatcgaagg cgcgatgaaa agtcactgcc agcggaccgg cagcagccat
    12361 tattttttcc attcgtggca tatcgacatt cccgtcaaca tcgagaacgc ccgtcaccag
    12421 tccaggaaaa cctaattcgc ggactgtgcg cacatcttca agaatggcgg caaactcacc
    12481 gtcgctgtag caaaaatcac caccgcgtgg gcggataatc ggatgcactg ggatcgtcac
    12541 ccgctggcgc acggatttca gtacccccaa cgacggcgtt aggcccccct cttttggagc
    12601 tgcgcataat tcaactctgt cagcgccgtt ttgctgcgcc gttagcgcgc attccatgct
    12661 gtaacagcaa atttccagta atgccatttt tgctccttag ttacgccgac tgctcgctgg
    12721 caacgatctc ttcaatggac cacggatgaa acttaatggt tgttttacca tcggtgaccg
    12781 ccagcgtcgg attcggtaat cgttcatgct caccttttgg cgaactggtt ttaactgaga
    12841 tccccggcag gctgagtcct tcatcagaaa gcaatgccag cgcacgggca ttcagcgttt
    12901 ctggatcacc cggtagaacg atttcaatat gttcccaacc ttcgtgcggg taacgttttt
    12961 ccccgggcca cggtagctcc acaatggaaa actgccagtg cgcaacctgt agcggttcat
    13021 gcaatttaaa caaacaaatc ggtctgccat tgatcatatt ttctgacaaa agctcgccgc
    13081 actgttcaaa cccgcgacgc cagcgttcag cggtgacgtt ttgatggcaa cgcaaagaaa
    13141 tgtgatcggc agtcagcgga gtgatatcca accccagacg gcgggaaagt tcatctaatg
    13201 cgtgggtaaa tcgcggtaaa tccgatgcaa tatcctgcag ctcgtcgata gattgccagt
    13261 tcgccataat cattcttcgt ctttcattaa aagcgttaat ttaccctgtt gccctgcgcc
    13321 aaccaaccgc tgatttcacg ccgcttctga tgcaatagtg aaaacggcaa tacgccacgc
    13381 gcacgttgct gacgaaaaca gccatttgca gtatactccc gccctaattt ctttaactgg
    13441 tgcgggtaat ttttgctcgc ttcatcaatg taaggtattc cggtgaatat tcaggctctt
    13501 ctctcagaaa aagtccgtca ggccatgatt gcggcaggcg cgcctgcgga ttgcgaaccg
    13561 caggttcgtc agtcagcaaa agttcagttc ggcgactatc aggctaacgg catgatggca
    13621 gttgctaaaa aactgggtat ggcaccgcga caattagcag agcaggtgct gactcatctg
    13681 gatcttaacg gtatcgccag caaagttgag atcgccggtc caggctttat caacattttc
    13741 cttgatccgg cattcctggc tgaacatgtt cagcaggcgc tggcgtccga tcgtctcggt
    13801 gttgctacgc cagaaaaaca gaccattgtg gttgactact ctgcgccaaa cgtggcgaaa
    13861 gagatgcatg tcggtcacct gcgctctacc attattggtg acgcagcagt tcgtactctg
    13921 gagttcctcg gtcacaaagt gattcgcgca aaccacgtcg gcgactgggg cactcagttc
    13981 ggtatgctga ttgcatggct ggaaaagcag cagcaggaaa acgccggtga aatggagctg
    14041 gctgaccttg aaggtttcta ccgcgatgcg aaaaagcatt acgacgagga tgaagagttt
    14101 gccgagcgtg cacgtaacta cgtggtaaaa ctgcaaagcg gtgacgaata tttccgcgag
    14161 atgtggcgca aactggtcga catcaccatg acgcagaacc agatcaccta cgatcgtctg
    14221 aacgtgacgt tgacccgcga tgacgtcatg ggtgaaagtc tctacaaccc aatgctgccg
    14281 ggtatcgtgg cggacctgaa agccaaaggt ctggcagtag aaagtgaagg cgcgaccgtt
    14341 gtattccttg atgagttcaa aaacaaggaa ggcgaaccga tgggcgttat cattcagaag
    14401 aaagatggcg gctatctcta caccaccact gatatcgcct gcgcgaaata tcgttatgaa
    14461 acactgcatg ccgatcgcgt gctgtattac atcgattccc gtcagcatca acacctgatg
    14521 caggcatggg cgatcgtccg taaagcgggc tatgtaccgg aatccgtacc gctggaacac
    14581 cacatgttcg gcatgatgct gggcaaagat ggtaaaccgt tcaaaacccg cgctggcggt
    14641 acggtgaaac tggccgatct gctggatgaa gccctggagc gtgcacgtcg tctggtggca
    14701 gaaaagaacc cggatatgcc agccgacgag ctggaaaaac tggctaacgc ggttggtatt
    14761 ggtgcggtga aatatgcgga tctctccaaa aaccgcacca cggactacat cttcgactgg
    14821 gacaacatgc tggcgtttga gggtaatacc gcgccataca tgcagtatgc ttacacgcgt
    14881 gtattgtccg tgttccgtaa agcagaaatt aacgaagagc aactggctgc agctccggta
    14941 atcatccgtg aagatcgtga agcgcaactg gcagctcgcc tgctgcagtt tgaagaaacc
    15001 ctcactgtgg ttgcccgtga aggcacgccg catgtgatgt gtgcttacct gtacgatctg
    15061 gccggtctgt tctctggctt ctatgagcac tgcccgatcc tcagcgcaga aaacgaagaa
    15121 gtgcgtaaca gccgtctgaa actggcacaa ctgacggcga agacgctgaa gctgggtctg
    15181 gatacgctgg gtattgagac agtagagcgt atgtaatcgc gttatacggc aacagccgat
    15241 gtcattgcag acatcggctt ttttatcatt aacgataatt tacaatcact tcattccgtt
    15301 gaatctttag cggcggagtt agccgcccac cgccaggcac ttcccagata aagcgcaacg
    15361 gttctgccgc cggtataccg gaaaaggcca tggtggttcc actctgcccc tccaattcga
    15421 cacaacgaga ttgtgaacac aagcgaaccc gcagtcctgc aggtgtcgga ccgataagct
    15481 gataacgcca cgccaccagc gtcatcaatc ctgaagcagg ttgtcgcgta gaaagaggcg
    15541 ctgacgacat cgattcaccg cgatgattta gcgtaacgcc cacgctactc gcctgccaca
    15601 tcccctcccc ggcggcttgc accagcagcg gaaacaataa tattgctaat aaggctctca
    15661 ttatttgccg ccaatcgtcg ccgtcatacg gatatgtcgg ttatctgata gttccagatt
    15721 cgacagcacc actaactgcg gcaagctgcg gcgcaggaag cgagacaata atggtcgtag
    15781 cgcgtggttc accaacaata ccggcggcgc acccaacatc tcctgacggg atagcgcttc
    15841 ctgagtttgc gccagtaaac gatccgccag ccctggctcc agtccaccac cgccttgcaa
    15901 cgcctgtagc aacaaacgtt ccagcggtgt atcgaggcca ataacatgga cttcatcttt
    15961 gccaggaaac cactgctggg taatcgcccg tcccaacgct acgcgcacga cagcggttaa
    16021 ttcatgcgga tcgctttgga tgggcgcatg ttccgccagc gtttcgagaa tggtgcgcat
    16081 atcgcgaatc ggcacttttt catcgagcag attttgcagc actttatgca gtgtggtaag
    16141 cgtgacgacg ccaggaacga gatcttccgt cagctttggc atctcctggg cgacgcgatc
    16201 cagcagctgt tgtgcctcct gacgaccaaa cagctctgcg gcatgctggc taatgaggtg
    16261 gttaagatgc gttgccacca ccgtgctggc ctcaaccacg gtgtacccct gaatctgcgc
    16321 ctgttcttta agcgcacttt caatccagat agcattcagg ccaaatgccg gatcaacggt
    16381 cgcttcacca ggtaatgttc cggcggcagt gcctggatta attgccagcc agcgccccgg
    16441 ataagcatca ccactcccca tttcgacacc tttcatcaag atacgatagc gggctggttg
    16501 caaatccatg ttatcgcgga tgtgcaccac tgggggtagg aagcccatct cctgggcaaa
    16561 tttcttgcgg atactgcgta tacggcccaa caactcacca tcctgctgaa aatcaaccat
    16621 cgggatcaac cgatacccca cttccattcc cagagaatct tccagttgta catcgttcca
    16681 cgttgcttcg actaccgaat tattctctgc catttttacc ggttttggtt cggcaggcgc
    16741 tttttgttca cgtccgcgga tccaccaggc cagccccagc aatccggcag tgaacagcaa
    16801 aaataccagg tttggcattc caggcaccag gccgagtaaa ccgagcacgg cggcgcttaa
    16861 taacataacg cttgggttac tgaaaagctg attcaccatc tgctcgccaa catcctgatc
    16921 ggtgctgaca cgcgtaacga tgacccccgc ggcggtagaa atcaccaacg ccggaatttg
    16981 tgccaccaga ccgtcgccaa tggtcaatag cgtataactt tccgccgcgt gccccatgct
    17041 catgccatgt tgcagcacac cgaccagcaa tccgccgaca acgttaatga ccatgatgag
    17101 gatcccggcg atggcatcgc cgcgaacaaa cttacttgcc ccgtccatcg agccgtaaaa
    17161 atcggcttcc tgagtcactt cggagcggcg ttttttcgct tcatcttcac caatcaatcc
    17221 ggcgttaagg tcggcgtcaa tcgccatctg cttacccggc ataccatcga gaacaaagcg
    17281 cgcgcccact tccgcaatac gcccggcacc tttggtgata accataaagt tgatgatcac
    17341 gagaatgacg aacaccacga taccgatagc gaaattgcca ccgacgagga agtgaccgaa
    17401 cgcttcgacc acctttccag ccgccgccgc gccggtatgc ccttccatca aaatgatacg
    17461 ggttgaagcc acgttaagtg ccagacgcaa cagcgtggta aacaacagaa tggtcggaaa
    17521 cgcagcaaac tcgagcgtgc gctgggtaaa catcgccacc agcaacacca tgatcgacaa
    17581 ggcaatattg aaggtaaaca gcaggtcgag tatgaatgcg ggcagtggca gcaccatcat
    17641 cgacaagatc aacaggatca aaatcggtcc ggcaaggatc tgccattgtg tcgatttcag
    17701 gtttgcgggc aggcgcagca tcgcggccag attactcatg ggtcggtttc tcgttaataa
    17761 aatccagggc ttccggcacc ggaagatgag taggttgtac agggcgctgt ccaccagcca
    17821 gacgccagcg tttcagttgc cagacccagg ccagcacttc cgccaccgcg gcgtacagtt
    17881 gacccgggat ttgttgaccg atctccgcat gtcgatacag cgcacgcgcc agcggcggcg
    17941 cttcaagcgt cgggacgtta ttttcagcgc caatttcacg aatgcgcagc gcgaccagcc
    18001 ctgcaccttt agcgaccact ttcggtgcgc tcattttgtt ttcgtcatac tgcaacgcta
    18061 ccgaatagtg ggtcgggtta ttgacaatga catccgcttt cggcacatcg gccatcatcc
    18121 gacgccgtgc ggccgctcgc tgcatctgac ggatccgccc tttaacatgg gggtcgcctt
    18181 cgctttgttt gaactcatca cgaatatcct gccgtgacat tcgcagcttt ttcaggtggc
    18241 tgaagatttg gaaaaagacg tcaaatccca ccattggaat gacaccaagc accaccagca
    18301 gcgcgcatag ccctaccaga tccattgcat tacccatggc ggtaatcgga gactcggcca
    18361 ttaagcgcat catctgcggc cagtgatacc agagaaaaaa ccccgtcacg ctgccaacca
    18421 ggatggtttt caaaattgcc ttaagcaact ccgcgccagt ctgtgccgag aacgtccgtt
    18481 taatgcccgg tagcgggttg agtttggaaa acttcggctg caaggatttt ccgctaaata
    18541 ccagcccccc cagcataacc ggagaaataa tcgccaccag caccacgccg ctaatcagcg
    18601 gtagcagcgc cagcatggct tctctgatca gcagaataat ctgcccgagg atcagattcg
    18661 ggtcattgat aatactgtga tcaaaatgta gccctgcgga gagcatgccc gacaatcgac
    18721 gggccagcga cacaccgcca aaccagataa cactaacgcc caccagcaaa atcagcagtg
    18781 aagtcagttc acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag
    18841 gtgtgggggc ctctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag
    18901 tattaagcgt aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac
    18961 aggcgggatt tagggctttt actgccacac atcaagcata gtatgcgttt gtcggatgcg
    19021 gcgtcatcgc ctgatatgac gtggtcacgt cacatcaggc aatacaaatc aaaatccaag
    19081 actatccaac aaatcgtcca cctgaccctg actggctacc acaccggctt tgctggtatc
    19141 gacctgaggt ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg
    19201 gatgttttcc aacaacacca tcagcaactg acgttcgatc tcctgaatga catccatcat
    19261 ccgcttaatg acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag
    19321 ttgcgcgtta gtaaagctgg tatgcgcggg gacatctgcc agaaattgcc gtgtatcgat
    19381 caccagttca cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg
    19441 ggttaacgct tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact
    19501 gttcagcgcc cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg
    19561 cgcatcgggg atggcttccg ctgcttcggc aatggcctga tccagcccca gttcccgcaa
    19621 actgtcgcgc agcatacgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg
    19681 ctcgtcagca ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct
    19741 caaagatttt gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc
    19801 cactggcccc cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca
    19861 ttaacactgg caatgccgac atcgcgccat ccgcacgaat cgttttcagc aactccaggc
    19921 catccatatt gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact
    19981 tattgagagc gtcgacgcca tcttccgctt cctcaacatt attgaatccc agctctttca
    20041 gcaggttacg cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt
    20101 ctttatccgc catttcacac tcctgattta aatacgtatc gcctgtccgg cactaatttt
    20161 tgccagcatt tgctgactta cctggctaag atcgaccact tcgcagacac cacccatatt
    20221 gatggcctcg cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca
    20281 tgcccccgcc tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggttag
    20341 gattacccca accgcattac gccccgcctg tttggccaca gaatggaaca acacatctac
    20401 cgatggccga tggcgattaa ccgccgggcc atcgtgaatt ttgatttgat aatttgcgcc
    20461 actacgcgcc agctccatat gccgatcgcc cggcgcgata taggcatgtc ccggcaagac
    20521 gcgctctcca tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc
    20581 aaaagagcgg gtgaaaccgg gcggcatatg ctgggtaatt aacagtgccg gactggaaag
    20641 cggcaacggt tgcagtacgt gacgaattgc ctcagttccc cccgttgaag caccaatcgc
    20701 aatcagtttt tcagaactca acaacggccc cgccttcagc gttgtcggtg ccgacaatgg
    20761 cttatgtgct gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct
    20821 atacgccagc attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctatcgcccc
    20881 caactccagc gcgcgcagcg tgacttctga ccctttgccg gtcagggaag aaaccatcac
    20941 aacgggcatt ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg
    21001 catttcaaca tccagcgtca atacatcggg attgaatttc ttaatcaaat cacgcgcaac
    21061 cagcggatca ggggcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt
    21121 catgatctgg cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt
    21181 taatccttac ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcaaggtgg
    21241 ctaaagtttt cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cgaaacaaag
    21301 cggcgcaaaa tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt acgacagaag
    21361 atcgcatcaa acggccccgg cacggtgtac tgtttcgcca gcaaattcag cggggcaaaa
    21421 tcaacatagt tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct
    21481 cgcatgaaat accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgataa
    21541 ataccgcttc tggctttttc cagcacttcg gtgtcgatat cgctggcaaa cactttccag
    21601 cgtccgggcg cggtgcccaa tgtatcagcc agcgtcatcg ctatgctgta cggctcttcg
    21661 ccggttgaag ccgccgcgct ccatacgcga tactcgccag aacggcgacg tgcgtgatcc
    21721 gcgagcagag gaaaatggtg tgcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa
    21781 ttgataaacg cctgccactc accgctgtgc tgattagatt ccagcaagtt caggtaatga
    21841 ccgaaatccg ccagtcccag cgcacgcaaa cgacgaacca gtcggttgta aaccatgtcg
    21901 cgtttatggt cagccagaac gatcccggcg cgttgataga tcaattgact tatccgccga
    21961 aaatgcgcgt cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca
    22021 cagggcagag atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt
    22081 tgtaactgcg ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca
    22141 cgcgacgaaa gatggtcggc ctgattcagt cgttgcaaat cgtcgcgaaa tgcccaaaag
    22201 acatgccgtt actgccaatc tgcaagatcc cgcagagaat caaaattaaa aacagcgtgg
    22261 tcgaaattcg aatacgatta aacatcaacg ctccccatca ggcggcaatg accgctttag
    22321 taaatactcg tcaaaatgtt tcccagtttg gatcttgttc agcaattcgc agtcgtggct
    22381 gtgccggtgg ttgctcactg gcaggacggg atggtgtttg cggtttattg gtgagtgggc
    22441 tggctgccag acggaacgcg gaaaccgctt gcgttaaacg actcgcctgt tcttccagcg
    22501 cagctgcggc ggcagctgat tcctgcacca gcgatgcgtt ctgttgcgtg acgcgatcca
    22561 tttccgaaac cgccaatgcg acttgatcga tgccacgact ctgttcatcc gatgctgatg
    22621 caatctcgcc cataatgtca gtcacgcgag tgacagcatt gacgatattg ttcattgttt
    22681 cccctgcgct ttcgaccagc accgaaccgg tatcaacgcg cgagacggag tcttcaatga
    22741 gggctttgat ctcttttgcc gcctgggcgc tgcgactggc aagattacgc acttcgcccg
    22801 ccaccacggc aaaaccacgg ccttgttcac ccgctcgcgc ggcttcaaca gcggcattca
    22861 gcgcgaggat attagtctgg aaggcaatac catcgataac gctgataatg tcggcaattt
    22921 tcttcgaact atcggcgatc tcatgcatcg ttttcactac gccatccacc actttgccgc
    22981 cgtgctgggc ggtgtcggag gcactttgcg ccagttgcga ggcctggcgg gcgttatcgg
    23041 cgttttgctt cactgtcgcg gcgagctgct ccatgctggc ggcagtttct tccagcgcgg
    23101 atgcctgctg ttcagtacgg gaggaaagat cggtgttgcc cgccgcaatt tcacgggtac
    23161 cggcatagat ggcatctgaa ccttcgcgca catgagtgac ggtgtcagtc aaagagcgtt
    23221 gcatatgtga aacgctctgc gccaggtcgc ccatttcact gcgcccgtca atggtcaggg
    23281 tattcgccag gttaccaccg gcaatttcgc gaatatgcgc aataattttt gccagcggtg
    23341 taagcaacat acggcgaatg ccgtaccacg ccgccagcag aatcaatacc accaccagcg
    23401 cgataaccgc cagttgccac tgggcaaatc ggtaatcatc tgcgttgtca gtgacgatat
    23461 cgcgatacag tttttcactg ctgagggcgt actgagcaaa cgcttctccc attgcatttt
    23521 gcattccctg ggttggctga gcgaaataag ctccagtatt gccataatca agataatcaa
    23581 tcagttcagt taacgctgtg tgatagtttt tatatttttc atcaatatta cgactggtag
    23641 cgaccatttc aggtaacggt gccatgcttt tgaatttttt ataatgagtc gcggcctggg
    23701 ccaatgtttt cctggcgcta tcgagcaatt caactttggc attactttgc tgattggatg
    23761 aatccatcat catccgcacc gctgaacgac tcaggttaat gcgcgtttgt agcattaaat
    23821 cccatgttga cgtcagctcg ctctgctgtt cccgtaattg attggaaacc acaaagctct
    23881 tctggctatg gtgaagggaa gaaaaaaaca gactgccgga aataagctgt aacagtgcga
    23941 atacccccag caccattacc aacagcgtga ctacgcggat acggttaatc ataaggcacc
    24001 ttcctgaaaa caagttgatc tcgttatcgg caaggagggg ggaaacttta ttgctgatgc
    24061 cacccgccgc gaaattgaaa taaaaaaccc gatgcgcaga tcatcgggtt catttcaatt
    24121 gaggacatcg ggagaattac gccacttctg acgccgcgct atctaacagc gccatctctt
    24181 cgctgttcag cagtttttcg atgttcacca gaatcaacat gcggtcgccc agtgcgccca
    24241 gtcctgtgag atattcggtt gaaagcgtca cggcaaattc cggtgccgga cgaatttgct
    24301 ccgccgtcaa tgaaagcacg tctgagacgc cgtcaaccac gatgccgacc acccgctgtc
    24361 cgagattcag gacgataact accgtgttgt cgttatagtc cacatccacc tggctgaact
    24421 taattcgtaa gtcaacaatc ggcacaataa cgccgcgcag attcgtgacg cctttgataa
    24481 acgctggcgt gttcgcaatc cgtgtcacct gatcgtagcc acggatctcc tgcactttca
    24541 gaatatcaat accgtactct tcatcaccaa gggtaaatac cagaaattcc tggcctgacg
    24601 gctcgctggc cagctttgtt acattcgtca taccggtcat attgttacct ttttactcat
    24661 tcaggcggcg gtgttcgcca tacgttgttc gcggtttatc gcctgcaagg cggagacatc
    24721 aacaatcagt gccacgctgc cgtcgccaag aatggtcgca gcagaaatgc cggggacttt
    24781 gcgatagtta ctttcaaggt ttttaaccac aacctggtgt tgaccaatta attgatccac
    24841 tagcaaggcg tagcggcgac cgccactttg taagatcacc acaattccct gggtggcttc
    24901 ggttttcgcg cccgcgacgt tgaacacttt ccacagttcg acgataggca gatattcacc
    24961 ccgcacttcc agcacccgct cgccgccagc cagtggatgc agatcggctt cacggggttg
    25021 cagtgattcc atgacagcat tcagcggcag aatgaaaact tcatccgcaa cgcgtacgga
    25081 catgccgtca agaattgcca gcgtcagcgg cagtaaaatg cggatcgtag tgccagtacc
    25141 ctgcatcgac tggatctcga catgaccgcc catctcctgg atattacgtt taacgacgtc
    25201 catgccgacg ccacgcccgg agacgtcggt gacctgctcc gccgtggaga agccaggtgc
    25261 aaatatcagc attgcaactt cgtcgtcgct catgttttcg ctgacggtca gaccttgcga
    25321 ggccgctttt gccagaattc gttcacggtt tagccccgcc ccatcgtcgg tcacttcaat
    25381 gcaaatgttg ccgccctgat gttcggcaga cagaattaaa tttccgacgc tgtttttacc
    25441 tgcagcgagc cgtttttctg gcagttcaat accgtgatcg aggctattgc gtaccaggtg
    25501 ggtcagcggg tcgataatgc gttcaatcag gctcttgtcg agttcagtag aactgcccac
    25561 cagcgtcagt tctacctgct tgccgagttt tcccgccaga tcacgcacca gccggggata
    25621 gcgactaaag acatattcca tcggcatcat gcgaatcgac atcactgatt cctgcaaatc
    25681 acgggcgtta cgttgtaact gccccatgct ggtaatcaaa tcaccatgat taaccgggtc
    25741 cagttcgctg gaacgctggg caagcatgga ctgggtgatg accagctcac cgacgaggtt
    25801 aattaattga tcaacctttt ctaccgctac acggatgctg gtggattcac tgctgcgcgt
    25861 cgttttttcc cgctccacgc gaccggttgg cgcttgttcg gctgccagtt taagcattgg
    25921 tgaggtggat atttttggcg agacgtctac tgtttcaaag gtaatctgat cggcttcaat
    25981 cacaaaacag agtaccgctg tgatgtcatc ttcggcgatg tcgcccggta atattgccga
    26041 gagggaatca gcccctttca ccacgtcagt taacgttgtc agatgcccca gctcttcttc
    26101 cagcaggtcg acttcgccgg ccttcaggcg cgaaaggata attcgtcgcg gtgactgact
    26161 acgactctgc tcatcttgtg gttcactttt ggcaaccaca cttaatcggg tcactgcgga
    26221 tggcgtttcg cctttcgctt ctaatgccag ttgacgcaag gcctggcaga tataatcgaa
    26281 gctggcggca tccggctctt gcgactgttt ataagcgtcg agctgttctt gcatgatgtc
    26341 cttcgtttcc aaaaacagat tgataatgtc ggtgttgagt tgcatctcac ctcgtctggc
    26401 ttcatcgagc aggttttcca tcagatgcgt ggtttcctgc aaaacgctga agccaaaggt
    26461 tcctgcccct cctttgatcg agtgggcagc ccgaaagatg gcattcaatt gttcggcatc
    26521 tggcgcttcc ggctgcaaaa ccagcagatg ctgctccata tcagccaaca gttcgtccgc
    26581 ttcatcaaaa aatgtctgat aaaaatcgct tatatccatg ctcacgctgt cacctcggtt
    26641 cggctgatgg cattgtggga acactgacct gtggtgcaac ctcaggtttt tccagggcgc
    26701 ttactggctc attctggctt tcggcgtttt catgcaaaat ggcctgttcg gcttgtttgt
    26761 tcagtaccag caggctgatg cgacggttga cggcatcatc aggtccgcga tcgcttaagc
    26821 gcatcgttgc cgccatgccg acgacacgta acactttgcc gccattcaac ccgccaacca
    26881 tcagttcgcg gcgggatgca ttggcacgat cggcagaaag ctcccagttg ctgtaacctt
    26941 tctcaccgct ggcgtaggga aaatcatcgg tatgacccga gaggctaatt cggttgggaa
    27001 taccgttcag tacgggcgcg atggcgcgca gaatgtcgcg catatagggt tcgacgtcgg
    27061 cactgccagt tctaaacatc gggcgattct ggctatcgat gatctgaata cgtagacctt
    27121 cctggaccag atcgattttg agatgtggac gtaacgcccg cagtttcgga tcggactcaa
    27181 tcaactggtc gagatcgcca cgcaatttcc gcaatcgact ttgctccatg cgttttttca
    27241 gctcttcgat gttcggccgc ttattcactt ccccctggct ttgggtgtaa tcatcaccac
    27301 cgccgggaat tgggctttca ctattagaaa tgcgatcgcc gcccgtaacc gctgtcgcca
    27361 gcggagtccg gaagtactcc gcaatctgaa tcagctcttt tggactggag atagagatca
    27421 gccacatcac cagaaaaaag gccatcattg cggtcataaa gtcggcataa gcaatcttcc
    27481 acgatccatg tgctgccccg tggcttttgg ctttacgtcg tttgacgaca ataatcggat
    27541 gcgcttgatt cttcatgctt cctcggttgt cgtctgttgt tgcggatttt tcaccgcacg
    27601 cacatgctct tccagctcaa taaacgacgg acgttcgctg gaatagagcg ttttgcgacc
    27661 aaactcaacg gcgataggcg gtgcgtaacc gttcagattt gaaagcagag tgactttgac
    27721 gcactgcatc attttgctgg tttcggcgct tttctgacgt aaaacactcg ctaatgggga
    27781 aataaatccg taagccaata aaataccgag gaaagtcccc accatcgcat gagcgataag
    27841 cgcccccagc tcggcggcag gacgatcggc tgaacctaac gcgtgaacta cccccattac
    27901 agccgcaaca ataccaaaag ccggaagtga gtccccgacc agcgccagac tgttcgccgg
    27961 gacttctgcc tcgctttcgt gcgtctcaat ttcttcatcc atcagggctt cgatttcgaa
    28021 ggtgttcatg tgaccgctga taatcaggcg cagataatcg acgataaatt caagcatgac
    28081 gctatccgca aggatgcgtg ggtagctggc gaagatctcg ctctcacggg gattttcaat
    28141 atcgcgttcc agcgaaaaca tccccatctg ccgcgatttc gccatcaacc gataaagcag
    28201 agccagcaga tccatataca ttgctttggt gtatttggag cgacgaaaca gcaacggcag
    28261 cgccttcagc gtgcctttaa tcgctttgcc attattgccg acgataaacg acccaatccc
    28321 tgcaccggca ataatcacca gttcagcggg ttgatagagt gctccaaggc ttccaccggt
    28381 catcaaataa ccgccgaaaa ctgtaccgag aacaaccagg taacctaata agataagcac
    28441 gacatcatcc ttccactgtt gaccatgaca ggatgttcag tcgtcaggcg ttaacacgcg
    28501 ataggggcaa aaaaaagcag cggtacgtcg ttaccgctgc tggaatgttt cgccacaccg
    28561 tatcagttaa acagcctgta ctctctgttc atccagcagt tgtgggataa tatcggcagg
    28621 attctgggaa agtttacgtc tttttactgc ccgggatggc ggctgacata agctgcaggc
    28681 aaagctgcca acaggctggt gagcatgggt aataaaattg ccgccgcagc agttgcagct
    28741 ggaaagttgc agtaatccac tttcaacaaa ccgcaccaat gtccaggcac gggttaatgc
    28801 cagcagcggt ccttcttctg cttgcgggca ctgttcaagg tataaacggt aggctttgat
    28861 caccgcatcg acgccattac acaagccagt tttcagtaaa aactgccatg cattacagaa
    28921 catcgaagca tgaacatttt gttcccaggt cataaaccag tcggttgaaa atggcagcat
    28981 gcctttcggc ggtgggcttc cgcgcagttc tttatagagt tttatcagac gtccgcgact
    29041 taactgtgtt tcgctttcca gcatctgcaa acgagcgccc agggtgatca attccattgc
    29101 cagctgaata tcccgcgctt cctgaacaat gcttttttca ctcatgatca ggcccttttc
    29161 ttgcgcagcg cttcttcagg ctgattaaca tcattcagca agcgtgttga gagcatgatg
    29221 ccggtatgaa tttgctggag atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc
    29281 tggtggctgt caaaacggaa gtgacaaacc agttgattgg tttctgccag cttaaccatt
    29341 tgcggaagag tcagtgccgc taacgttgtc gccatttctt catttatgcc gagacgaaac
    29401 atagcggacg ctttgtcctg aacaatcaga cgctgtgcaa gtagtaaata tgacaagttg
    29461 atgtcataaa tgtgtttcag caactcggag gtatgcatta ttcccaccca gaataaccaa
    29521 ctttattttt atgcggtctc accgcacccc gtgatgtcgc cgggaagccc cggtaaaaaa
    29581 taattagcat tagaatagtt gcgataagct gcaataagca gacccacctt tttggtttaa
    29641 taagtcctta caaatagaag ggggtcttta cacttatcta agatttttcc taaatcgacg
    29701 caactgtact cgtcactaca cgcacataca acggaggggg gctgcgattt tcaataatgt
    29761 gtgat
//
LOCUS       DACADF010000049        29339 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.049, whole genome shotgun sequence.
ACCESSION   DACADF010000049 DACADF010000000
VERSION     DACADF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.049"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            108..1691
                     /locus_tag="ISB30_002059"
     CDS             108..1691
                     /locus_tag="ISB30_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAP1989690.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2143..3996)
                     /gene="asmA"
                     /locus_tag="ISB30_002060"
     CDS             complement(2143..3996)
                     /gene="asmA"
                     /locus_tag="ISB30_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAP1989691.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTLSLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4018..4599)
                     /gene="dcd"
                     /locus_tag="ISB30_002061"
     CDS             complement(4018..4599)
                     /gene="dcd"
                     /locus_tag="ISB30_002061"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAP1989692.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4691..5332)
                     /gene="udk"
                     /locus_tag="ISB30_002062"
     CDS             complement(4691..5332)
                     /gene="udk"
                     /locus_tag="ISB30_002062"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP1989693.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5650..8967
                     /locus_tag="ISB30_002063"
     CDS             5650..8967
                     /locus_tag="ISB30_002063"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP1989694.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGKINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQVAAHSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPQARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELIELLENSPL
                     SPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9074..9922)
                     /gene="alkA"
                     /locus_tag="ISB30_002064"
     CDS             complement(9074..9922)
                     /gene="alkA"
                     /locus_tag="ISB30_002064"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAP1989695.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSVGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYVCFPTPQR
                     LAVADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10056..11408
                     /gene="yegD"
                     /locus_tag="ISB30_002065"
     CDS             10056..11408
                     /gene="yegD"
                     /locus_tag="ISB30_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP1989696.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALNQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALTEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11421..13361)
                     /gene="yegI"
                     /locus_tag="ISB30_002066"
     CDS             complement(11421..13361)
                     /gene="yegI"
                     /locus_tag="ISB30_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HAP1989697.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVESVAKIYHTS
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVMIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGNFVLAKVWAMVMASVAPPALQLPLPDHFQAAGRPLPSGLLRREYI
                     ILIEIALSGLSLLLCGLQTEPRYIILVPVLAAIWIIGSLTSKAYKVEVQRRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRDLAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVRGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLQAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13358..14119)
                     /gene="pphC"
                     /locus_tag="ISB30_002067"
     CDS             complement(13358..14119)
                     /gene="pphC"
                     /locus_tag="ISB30_002067"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HAP1989698.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQIQVAWLNDQQPLLSVFV
                     ADGAGSASQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLDTFTSTERAHKVAAFTDGIQRLALNMLDNSPHMPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14116..14775)
                     /locus_tag="ISB30_002068"
     CDS             complement(14116..14775)
                     /locus_tag="ISB30_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP1989699.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGSAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14993..15049)
                     /locus_tag="ISB30_002069"
     CDS             complement(14993..15049)
                     /locus_tag="ISB30_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAP1989700.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            complement(15321..15377)
                     /locus_tag="ISB30_002070"
     CDS             complement(15321..15377)
                     /locus_tag="ISB30_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAP1989701.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            15656..16903
                     /gene="mdtA"
                     /locus_tag="ISB30_002071"
     CDS             15656..16903
                     /gene="mdtA"
                     /locus_tag="ISB30_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HAP1989702.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKAASREDTKKGARS"
     gene            16903..20025
                     /gene="mdtB"
                     /locus_tag="ISB30_002072"
     CDS             16903..20025
                     /gene="mdtB"
                     /locus_tag="ISB30_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAP1989703.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSESVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20026..23103
                     /gene="mdtC"
                     /locus_tag="ISB30_002073"
     CDS             20026..23103
                     /gene="mdtC"
                     /locus_tag="ISB30_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAP1989704.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLVISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRMRFSRKPKQAVTE"
     gene            23104..24519
                     /locus_tag="ISB30_002074"
     CDS             23104..24519
                     /locus_tag="ISB30_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1989705.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMASIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24516..25919
                     /gene="baeS"
                     /locus_tag="ISB30_002075"
     CDS             24516..25919
                     /gene="baeS"
                     /locus_tag="ISB30_002075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAP1989706.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPIDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            25916..26638
                     /gene="baeR"
                     /locus_tag="ISB30_002076"
     CDS             25916..26638
                     /gene="baeR"
                     /locus_tag="ISB30_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAP1989707.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26818..27150
                     /locus_tag="ISB30_002077"
     CDS             26818..27150
                     /locus_tag="ISB30_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP1989708.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27297..28658
                     /locus_tag="ISB30_002078"
     CDS             27297..28658
                     /locus_tag="ISB30_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAP1989709.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29158..>29339)
                     /locus_tag="ISB30_002079"
     CDS             complement(29158..>29339)
                     /locus_tag="ISB30_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989710.1"
                     /translation="YTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVKLKTEG
                     AGLHGVARLTFKCGE"
BASE COUNT         6767 a         7862 c         7747 g         6963 t
ORIGIN      
        1 ctcaaataat atgagataaa tagtgctgca acattgcatt tttgccccga tttatccatg
       61 atcgaattgt gacatttgtc atacaacgaa taggttttgt acttactatg gaatggattg
      121 ccgatccgtc tatctgggct gggttaatca cgctgattgt gatcgaactg gtcctcggca
      181 ttgataacct ggtctttatt gccatcctcg ccgaaaaact gccgccgaag cagcgcgacc
      241 gcgcacgggt taccgggctg ctgctggcga tgttaatgcg cctgttactg ctggcgtcaa
      301 tctcctggct ggtcaccctg actcaaccgc tgttcagctt ccgctcgttt acctttagcg
      361 cccgcgattt aatcatgctg tttggtggtt tcttcctgct gttcaaagcc acgatggagc
      421 tgaacgaacg gctggaaggg aaagacagca ataaccccac gcaacgcaaa ggtgcgaagt
      481 tctggggggt ggtgacacaa attgtggtac tggacgccat cttctcactc gactcggtga
      541 ttaccgccgt cgggatggtc gaccatttac tggtcatgat ggccgccgtg gttatcgcta
      601 tcagcctgat gttgatggcc agcaagccgt taacgcaatt tgttaacagt cacccgacca
      661 tcgttattct ctgcctaagc ttcctgttga tgattggctt tagcctggtg gcagaaggtt
      721 tcggcttcgt cattccgaaa ggctacctgt acgctgctat tggtttctcg gtgatgatcg
      781 aggcgctcaa tcagttggct atctttaacc ggcgacgttt tctttccgct aatcagacgc
      841 tgcgccagcg gactaccgaa gcggtaatgc gcctgcttag tgggcaaaaa gaagatgcgg
      901 aactggacgc cgaaaccgcg tctatgctgg tggatcatgg taaccagcaa atctttaatc
      961 cgcaggaacg gcggatgatt gagcgggtac ttaatcttaa ccagcgtacc gtcagcagca
     1021 ttatgacgtc gcgccacgat attgagcata tcgatctcaa cgcgccggaa gacgagatcc
     1081 gccagttact ggagcgaaat cagcatacgc ggcttgtcgt tactgatggc gatgacgcag
     1141 aagatttgct cggtgttgtt cacgttatcg acctgctaca acagtcactg cgcggcgaac
     1201 cgctcaacct gcgggtgttg attcgccagc cgctggtgtt cccggaaacc ttgccgttgt
     1261 tacctgccct ggagcagttc cgtaatgccc gcacacactt tgcttttgtg gtggatgagt
     1321 ttggctcggt ggaagggatt gtgacattaa gtgacgtcac tgaaaccatt gccggtaact
     1381 taccgaacga agtggaagag atcgacgccc gccatgatat tcagaagaat gcggacggtt
     1441 cctggacggc gaatggtcat atgccgctgg aagatctggt gcaatatgtg ccgttgccgc
     1501 tggatgaaaa acgtgaatat cacaccattg ccgggctatt gatggaatat ttgcaacgta
     1561 ttccaaaacc tggcgaagaa gttcaggtgg gggattattt gcttaaaacg ttgcaggtgg
     1621 aaagccatcg cgtgcagaag gtgcagatta ttccgctgcg taaggatggc gagatggagt
     1681 acgaggtgtg atggcatcgg ccattttacc caatgctgcg taacgcctta taacttcagc
     1741 aatgaagcgt tgtctgtggt acatggacat cacagcgggg gtgtgggtcc ggctgtaaat
     1801 cgccgaatcg tttcctgtag gccggggcag cccacatgga tgtgggctga gggcgcgttt
     1861 tacagggatg ttacctcgcg cccgtacccg atagccgaaa ggaataagat gagggaaccg
     1921 cgccagcggc gatttaccgc cgggagcccg ggttgccagg gtggtggcga ttgagccacc
     1981 ctggcacgtt caccggcttg agcgttccat agtagcgagg aacatcaagt gaacggaaca
     2041 gccttcgatc ttacatattc agaatataat ggtgccgcca cgaacgcctg atgcgacgct
     2101 ttacgcgtct tatcaggcct acaggacagg tcacctggta acctacatct tctccagcaa
     2161 cttcttcaca tctttgccat tgcgggaatc tttattccgc tcggcccagt cgttaagacg
     2221 gcgttttgct tcatcctgta gatgtttacg cagtaactga tccacttgca ggctgtaatt
     2281 aagttgctgc cagttgccat aaacccgcag cggtactggc gtttctttga ggaaatcaat
     2341 cagtttgctt tccccgttcc agccacccac gacccgaata tcaaactggg tgtcgcaggt
     2401 ttgatctgcc agattcaaca tgccttcccc tgtcagcgcc agcacaggcg attgaccttg
     2461 catgtcgttt aacgtcacga cgccatcctt caacgtcaaa tcggtggtaa agcggtcaag
     2521 acgcgtcacg ttatcgaagt tttcagcggc cttcacatca ccaccattac gttctaccgc
     2581 ttgctgaatc atctgctgga agttcatccc ttccatgcgt gtatcagtca tttcgacatg
     2641 tgcctgtcct tgccagttgt ggcggaatgc gtcggcatct atgtcagcac cggagaaatc
     2701 accagccagt gacatctttc cggtcaacga aatcggatag ttaaacgcct tcagaatggt
     2761 gccaatctca acgttttcca gccgtggctg gaagtttatc cgcggattta ttgatgtcgc
     2821 gtccaacgtg cccggcagtg aaacctgtcc accgttaagt ttgccctgca gttgagtaat
     2881 ttccagcaaa cccgacttgt tggtcatttg cgtggcaaca tctgtaaaat tcattccgcg
     2941 ccagcgcacg ttactggcct gcaacaaaat atcagccgta aagccttgca gtccctgata
     3001 ggccggttca tcaatacgcg aagaaatgac cgggcgcggc aacgttgatt ggctctgccc
     3061 ttgctgcgcg gcaccgttct caccgttcgc tgtttcatta agggggatga ggttgtcaag
     3121 attcagttgc gggaattgca gtctcagctg ccattccggt ttctctgtca acgtgacctg
     3181 tgcttgcccg ctcagcgtac tgtcattggc ggtcaaactg atttggttaa atgaaaggcg
     3241 tttatgtgac tcctgccact gggcctggaa actcccctgc ccctgaatac cttgttttgg
     3301 taaatcggcc ccctgcaact gccagttaat ttgttcaata gccgccgtta aatcatgcgg
     3361 ataatcagaa gcatctactg taccgttaag ggataatgtg agatcgcgct gatcgcgatt
     3421 aacccgcccg gaaaactcaa atgagccacg atgttgggga tcttgttcca tttgcaggcg
     3481 gatattgcgg attgtcacct gctcgtcatc ttcatgctgg aacaccagaa cgctgtccgc
     3541 caccttaaga ctggatatat caaacgacca tccgcgatcg tctgacagat ccggcaaggt
     3601 attgtcgcgc ggtgcaaccg gagcgtcttc actgcgcacc gcttccgtct gcggcgtcag
     3661 ttggatcact gcccctttta gcatcacctg cttaacgctc agttgatgac tcagtagtgg
     3721 taaaagcgcc acgtccagac gcatgttgtc ggcgcgaacc agtggctggc ttgcgccctg
     3781 ggcggtgaga gacattcgcc cggagaggat actaagctgc ggccagacgt gccaacgcag
     3841 tggcccgtcg agctgcaatt gataaccgct acgtgcagca acttgcttga ccatatagtc
     3901 gcggaaatca ttcggattca ccagcaacac taacgcagat aacccggcca ccagcacgac
     3961 caggagtatc atcagcgtcg tcagaaatcg tctcatggta tcctcaatgg gcctgaatta
     4021 gtctttatcg attcggctgg ctaccgcgcc ctgctggttg cgatatttcg catcttcacg
     4081 gcggttgtaa ggtcgcgccg ccgggccgga gagcggctca aagctcagcg caccaattaa
     4141 catgcccgga cgcagcgcca gcggcagctt accggagttg taaaactcca gcacaatgca
     4201 gccagaccag cccggatcga tacggtgcgc ggtgacgtgc accatcagcc ccagacgcgc
     4261 cagtgaagaa cgcccgtcca gccagcccac cagatcggct ggcagcgtca ccgactccag
     4321 cgtcaccgcc agcgccagct ctcctgggtg aagataaaac gcctcgccct cgtcgagcac
     4381 gatttcatcg ctcatcacgc ggtcaagcgc ggcgctcact tcatctttgg gaccgctcag
     4441 atcgataaac gctgccgtgt gaccacggaa ggtacgaaat ttattgccca ggcgcacatc
     4501 caccgtcgcg ccgttaatac gctccactgg cggacgtggg ttgatcgaca aacggccttc
     4561 atcaagccag gcttcaatat ctcggtcaca cagacgcatg gcattttctc ctttcgagca
     4621 tcactccctt aacgccaatt acgtcaaggg cataccaggt tatcactgaa cggtacacaa
     4681 tttatcaagc ttattcaaag aactgactta ttttcgcttt caatatatcg atcgcgatgc
     4741 ggtttttccc gccgcgcggc acgataatgt ccgcatattg tttagaaggc tcaatgaatt
     4801 gcaggaacat cgggcgcacg gttttttgat attgcgccat cactgaatcc attgaacgcc
     4861 cacgctcgtt aacgtcacgc ttgatgcggc gcatcagaca gatatccagc ggggtatcaa
     4921 cgaaaatgga gaagttaagt tcgtcacgta aacgcgcatc cgtcagcaac aaaatgcctt
     4981 cgagaatgat gaccttcttc ggctcaaccg tcaccgtttc tttcatacgc gtatgttcaa
     5041 catagctgta aaccggcagg tcaattgccg agccgcgttt caacgcttgt aaatgctcaa
     5101 gcagcaggct gtgatccatc gcgctgggat ggtcgtagtt ggtcttaacg cgttcttcca
     5161 tcgacagatg gctttgatct ttgtaatagc agtcttcggg aattacgccg atgtgttcat
     5221 caccgacttg ctcacgtaat tcacgataaa gggtactggc aataagactc ttgccggaag
     5281 ccgatgcgcc agcgataccg ataatgacgc actgatgaga ctgatcagtc atatatttag
     5341 cgacctgatt aacctggatg ttaggaaggg ggcgacgaag cgccaaacgc ggcaattata
     5401 gggatttcat ccgcctgata ccagtcgaat agcgttgccg cgcgctcaga gttaattgtt
     5461 gacgaagaat tcccggtggc aaattacgtt gatcagtttt atacaaggta aaaaatgtta
     5521 tacgcagttg cgcaaattat ccgcctttac gtcactttat gagcaattcg catataaaat
     5581 gtaaaacttt tgtactagca tgaacacaga aacgaatact gacgacctgg ccttgcggat
     5641 aaagcggtaa tgagcaaaca atcacagcat gtattaattg ccctgcccca cccgctgctt
     5701 cacctggtca gtttaggttt agtctcgttt atcttcaccc ttttctcact tgagctttcg
     5761 cagtttggca cccaactcgc cccactgtgg ttcccgacgt ccatcatgat ggtggcgttt
     5821 tatcgccatg ccgggcgcat gtggccggga attgcgctga gctgctcgct gggaaatatc
     5881 gccgcatcca tcctgctttt ttccaccagc tcgctgaaca tgacctggac gaccatcaat
     5941 attgttgaag ccgtggtcgg ggcagtgctg ctacgtaaat tgctgccgtg gtataacccc
     6001 ttgcaaaatc tggctgactg gctgcgtctg gcactcggca gcgccattat tccgcctctg
     6061 ttggggggtg ttctggttgt cctgctgacg cccggagacg atcctctcag ggcatttttg
     6121 atatgggtac tgtcagaatc catcggcgct ctggcactgg tgccgctggg attgttattt
     6181 aaaccacact atctgctgcg ccatcgcaac ccaaggttgc tttttgagtc gctgctcacg
     6241 ttagctatca cgctgacgtt aagctggctt tcgatgctgt atctgccgtg gccttttact
     6301 ttcattattg tgctgttgat gtggagcgcc gtgcgcctgc cacgaatgga agcctttttg
     6361 atcttcctta ccacagtgat gatggtatcg ctgatgatgg ccgcggatcc ctctctgctt
     6421 gctacgccgc gtacgtacct gatgagtcat atgccgtggc taccgttttt gctgatcctg
     6481 ctgcccgcca acatcatgac catggtgatg tatgcctttc gtgcggaacg caaacacatt
     6541 tccgaaagcg aaacccgctt tcggaacgcg atggaatatt ccgccatcgg catggcatta
     6601 gtgggcaccg agggacaatg gctgcaatcc aacaaagcgc tctgccagtt tctcggttac
     6661 agtcaggaag aactgcgcgg actcaccttt cagcaactga cctggccgga ggatctcaat
     6721 aaagatctgc aacaggttga aaagctgatc agcggtaaaa taaacaccta ttcaatggaa
     6781 aaacgctact acaaccgcaa tggcgatgtt gtctgggcgt tgcttgccgt ctcactggtg
     6841 cgccacacgg atggcacgcc gctctatttt atcgctcaga ttgaagacat taacgagcta
     6901 aaacgcaccg aacaggtgaa ccagcaactg atggagcgca tcacgctggc taacgaagcg
     6961 ggcgggattg gcatctggga gtgggagcta aagccgaata tttttagctg ggataagcgg
     7021 atgttcgagc tgtatgaaat tcctccgcat atcaaaccga actggcaggt gtggtacgag
     7081 tgcgtgctgc cggaagatcg ccagcacgcc gaaaaagtga ttcgcgattc attgcaatca
     7141 cgctctccct ttaaactgga atttcgcatt accgtgaaag acggcattcg ccatatccgc
     7201 gccctcgcta accgggtact gaataaagaa ggcgaagtcg aacgcctgct cggcattaat
     7261 atggatatga ccgaggttaa acagcttaac gaggcattgt ttcaggaaaa agagcgcctg
     7321 cacattacgc tggattccat cggtgaagcc gtggtctgta ttgatatggc gatgaaaatt
     7381 acctttatga atccggtggc ggagaagatg agcggctgga cgcaggaaga agcgttaggt
     7441 gttccgctcc tgacggtgtt gcatattact tttggcgaca acggaccatt aatggagaac
     7501 atttacagtg ccgacacctc acgttccgcg attgaacaag atgtggtgtt gcactgccga
     7561 agcggcggca gctacgacgt gcattacagt attacgccgt taagtactct ggacggcagc
     7621 aatattggtt cggttctggt gattcaggac gtcaccgaat cgcgcaaaat gctgcgccag
     7681 ctgagctaca gcgcctccca tgatgcactg acgcatctcg ccaatcgagc cagttttgag
     7741 aagcaactac gcatcctgct gcaaacggta aacagtacac atcagcgaca tgcactggtg
     7801 tttatcgatc ttgatcgctt taaagcggtg aatgacagcg ccgggcacgc cgctggcgac
     7861 gctttactgc gcgaactggc gtcattgatg ctgagtatgc tgcgctccag cgacgtgctg
     7921 gcgcgactcg gtggcgatga atttggtctg ctgctgccag attgtaatgt cgaaagtgcg
     7981 cgttttatcg ctacacgcat tatcagcgcc gtgaatgact atcactttat ctgggaagga
     8041 cgtgtacatc gggtaggtgc cagtgcaggg attaccttga ttgatgacaa caatcatcag
     8101 gcggctgaag tgatgtcgca ggctgatatc gcctgttatg cctccaaaaa tggtggccgg
     8161 ggccgggtga cgatttacga accgcagcaa gttgccgcac atagcgaacg ggctgtgatg
     8221 tcgcttgatg aacagtggcg gatgattaaa gagaatcagt tgatgatgat cgcccacggt
     8281 gtcgcttcgc cgcggatccc gcaagcgcgt aatttgtggc tgatttcact taagctctgg
     8341 agttgcgaag gcgagattat tgatgaacaa acatttcgtc gtagcttcag cgatccggca
     8401 cttagccatg ctcttgaccg acgggtattc cacgattttt tccagcaggc cgcaaaagcg
     8461 attgccagta aaggcttaag catcgccctc cccctttccg ttgccggttt gagtagcgcc
     8521 acgctggtga atgaactaat tgagctgctg gaaaatagcc ctctatcacc acggttatta
     8581 catctgatta ttccggcaga cgcgatttta gatcacgcag aaagcgtgca aaaactgcgg
     8641 ctggcgggat gtcggatcgt attcagtcag gtgggccgcg atctgcaaat cttcaactcg
     8701 ctgaaagcga atatggcaga ttacctgcta cttgatggtg agttatgcgc caacgtgcag
     8761 ggaaatttga tggatgagat gctgattacg atcattcagg ggcacgctca gcggctcggg
     8821 atgaagacca tcgccgggcc agtcgtttta cccttagtga tggatacgct ttctggcatc
     8881 ggcgtcgatc tgatttatgg cgatgtgatt gccgatgccc aaccgctgga tttgctggtg
     8941 aatagcagtt atttcgcgat taactaaggg agcgtttgcc cattgcctga tgcgacgcta
     9001 acgcgtctta tcaggcctac aaatcgctca ttccccaggc cggataagct cgcaccgcat
     9061 ccggcaacca acgtcatgct tcgtctggtt gccagccttc cgtataccag atatgcaaca
     9121 acgcataaga acgccagggc ttccagcgtt cggcataacg gcggatttgt gccggtgtca
     9181 ttcccggaaa tcgctgttta atcagataat catccggcag aaaaacatct ttcgcctgcc
     9241 agccacgcaa agcaaaataa tttgccgtcc agcgcccgat acccggaaag gtttgcagcg
     9301 ttttcatcgc ttgttctacg tcgcccggta ttgtcattgg taaggtgccc tccagcgccg
     9361 catttgccag atgaatcagc gcctctgccc gtttcaacgg catacctaac gcttttaatg
     9421 cctgcgggtc ggctactgcc agccgctgag gcgtggggaa gcagacataa tccggaaaat
     9481 catccagccg ttcgccataa agctgtgcca ctctggaggt caattttgcc gccatcgcca
     9541 cgctcaccag ttggcctaaa atcgcccgca cgccctgctc aaaagcgtca acacaaccag
     9601 gtaaacgcaa tccgggccgc cccgcgccta actttcccaa cgcaccgtta acaatctgcg
     9661 ggttacattg cagatcaaac aggcggctca ttttcgccag acactcagcg gcaacaggtt
     9721 ctaaacctac acttaaattt atgtgcagag tatggcgggc tatatccgga atagcagtca
     9781 ccacgccgcg atattcgcct accgccagac tacgggcata ataactgtcc gcgaccgttt
     9841 ccacgccgct caccgcacgg gcggcgagaa atcccaacat ccacgaccag tcatacggcg
     9901 gctgccagtt cagggtatac atcgcatctc cttgttaatc cgctttcagc ataaacgtta
     9961 ttcagacgtt acgctttgct ttcatattcc ggttgtcgcg acggcaacat ttcgctaaag
    10021 tcacgcccct tcttcaccgg catggggatt atttcgtgtt tattggtttt gattacggta
    10081 cagcaaactg ttcagtggcg gtcatgcgcg acggtaaacc gcatttgcta aaaatggaaa
    10141 acgacagcac gctgctgcct tcaatgcttt gcgcgccaac gcgtgaagcg gtaagcgaat
    10201 ggctgtaccg ccatcatgat gttccggcag acgacgatga aacgcaggcg ctgttgcgtc
    10261 gggcgattcg ttataaccgc gaagaagata tcgatgtcac ggcgaaaagc gtgcagttcg
    10321 gtctttcttc actggcacaa tacattgatg atccggaaga agtgtggttt gtgaaatcac
    10381 caaaatcgtt cctcggtgcc agcggcttaa aaccgcagca ggtagcgctg tttgaggatc
    10441 tggtctgcgc aatgatgttg cacattcgcc aacaggcgca ggcgcagctg ccggaggcaa
    10501 ttactcaggc ggtgattggt cgcccgatca acttccaggg gctgggcggt gatgaagcaa
    10561 acacccaggc gcaagggatt ctggaacgcg cggcgaagcg tgccgggttt aaggatgtgg
    10621 tattccagta cgagccggtc gcggctgggc tggattacga agccaccttg caggaagaaa
    10681 aacgagtgct ggtagtggat atcggcggtg gtacgactga ctgttcattg ctgctgatgg
    10741 ggccgcaatg gcgttcgcgt ctcgatcgtg aagccagcct cctgggtcat agtggttgcc
    10801 gtattggtgg taacgatctg gatatcgcac tggcgtttaa gaacctgatg ccgttactgg
    10861 gcatgggggg cgaaaccgaa aaaggcatcg ccctgccgat tctgccgtgg tggaatgcgg
    10921 ttgccatcaa cgacgtacct gcgcaaagtg atttctacag tagtgccaac ggtcgtctgc
    10981 ttaacgatct ggtacgcgat gcccgcgaac cggaaaaagt ggccctgtta cagaaagtct
    11041 ggcgtcagcg tttaagctat cgcctggtac gtagcgcaga agagagcaaa attgcgcttt
    11101 caagcgtagc ggaaacccgc gcctcactgc cgtttatcag cgatgaactg gctacgctga
    11161 ttagccagca agggctggaa agcgccctca accagccact ggcgcggatt ctggaacagg
    11221 tgcaactggc gctggataac gcccaggaaa aaccggatgt tatctatctg accggcggta
    11281 gcgcccgttc accgctgatt aaaaaagcgc tgacagaaca gttgccgggc attccgattg
    11341 caggtggcga tgactttggc tccgtcaccg ccgggctggc acgctgggcg gaagtggtgt
    11401 ttcgttaatt gaaaactggc tcagcaacgg cttaaatcag cctgcgcctg agccagtttt
    11461 tctgccgcct gacgtaacgg ttccatcaac ggcatggtcc gtgctggagc ttgaaggcgg
    11521 aatcgctgca actctgtcgc accgacagtc agcgccgatt ccagccgatg tcgtttggcg
    11581 gccatttttg ccattaccgc ttgtctgtct gcaggcgtta ccgcttcgtt cggcctgaaa
    11641 acaaaacggc gttcgcaact cgctttccag tcgataaccg cctgggtcag atgatcacca
    11701 aaacccctaa cttgcttaac gctccgtcgg gtaacgtccg ctgctgtttc aataccaaag
    11761 gaccgtaacg ccgctttacg cgcagggcca acgccgggaa tagaggcaac atcaataaaa
    11821 aatccctcca gaaacttctg cttctgccgt tcccttgcgg tgtcgtgaag tgctgccaga
    11881 tcgcgctttt cttcttccgg taacccaaga atttcgtcct tcattttttc gagcatcgcc
    11941 cgtttggcga taaaaccttc cagcccgccc aactgttgga tctggctgac taaatggtca
    12001 tagtccattt ttgcgcgatt aaaagcctct cttcgtcgct ggacttctac tttgtacgct
    12061 ttgcttgtca ggctgccaat aatccagata gccgccagca cgggaaccaa aatgatataa
    12121 cgcggttctg tctgaaggcc gcaaagcagc agcgataacc ctgacagtgc gatctcaatc
    12181 agaatgatgt attcgcgcct taacaggcct gaaggaagcg gcctgccagc agcctggaaa
    12241 tgatcgggta atggcaattg caatgctggc ggtgctactg acgccatcac catcgcccag
    12301 actttcgcca gcacaaaatt cccgccggtg gtaatgacct cttcgccgag atcaataaaa
    12361 tagataacac cttgattatc cagcgcacac catgggcagt cagccaaatg accgggataa
    12421 acatgcattg ccgaaacggt acatttcttt aactgttggc gtagtaaatc aagtgccgct
    12481 acccacgctt ttgccgtcgg acgcccggtt gccacgccac tttccgtgaa tgcctgctga
    12541 aacatggctt caacatcgcc cggtaacatc gacagcggaa tcgatcgtgg cggcggtttt
    12601 aaaccacgtc gctgattatc tgacgcgtag gcataacgga aatgggcaat atccgtctcc
    12661 agcgcattac ccgcatcaga gataagcggt acaccggaat aaggatgccg cccaccaaac
    12721 aagacgtgaa atatcagcaa cgcaaggcca aaattatcgt gattttctgt gcgttcaaag
    12781 ccgacaaatg atggcatggt ttgcagctct ggcggcgtaa aatgcgacac gccgacttcg
    12841 cataaatgca gtgtaccatt ggcattaatc tgaaaggagt cgctatcaat catcaccact
    12901 ttgctgtcgc gacctaccat aaagctgttc tggtttacgt cacccacgac gtgcccgtgc
    12961 tcgtgaaccg tagcaaaaga tgaagcaata ttgcgcgcaa catagagtag aaaatcccac
    13021 gcacaatgag ggtaactctg gcgacgatgt gccgggctat agatcatatg aatcggttct
    13081 ttaccagaaa cttttggcat cataaaaccg ataacttttc cacctcgtcc accgtgaagc
    13141 gttgcctgcg gccaggcgac ataattcaac aactgcgcgt cagctgtcgc agccataaag
    13201 gcaagtttgt cctgttttaa ggcgggaggc gacgtgtgat aaatcttggc gacgctctcg
    13261 acaaactcct cgatatcata aaccgcacct tcgccgcctt tgcccagttc acggcctggc
    13321 gtaacgcact caccagtagc agtatataaa gtgggtttca tggtaaccac agcgccagtg
    13381 ccaacgtttt atcatcatcc gtacgctcgt ttaccgccgg gctggacaaa aactgcttta
    13441 acaattcagg taataaatcg agttgttcct gcgttgctga cgccagtcca ttgaaaaacg
    13501 gggtaaagaa aggcatatgc ggagaattat ccagcatatt taacgccagt cgctgaatac
    13561 catccgtaaa tgctgcaact ttgtgcgcac gctcagtgct ggtgaaagta tccagtctgg
    13621 aaacggcatc ttcatcagta atgaagtgcg tcatattggc atattccccc accatcggcg
    13681 tgagcggcag ttgcaaaccg tgacccaaat ctaccaccac gccgccatcg ccaatctgca
    13741 taatcaaggt gccattagct gatgaaatca atcccaaaaa cgtgcaggca aaatcgcgaa
    13801 ccgctaattc tttggcttcc gcctcggcaa aaagtcgctg acgaatggtc agcacaatgt
    13861 ccgttgcgag gacatcatta agccccaatt ccccgccctg cactttttgc gacatataag
    13921 ccatcgcttc attgacggcg agcatcgcac cttcgcctcc ctgcgaggcg ctacctgcgc
    13981 catcagcgac aaataccgac aataatggct gctggtcatt tagccaggca acctgtatct
    14041 ggcaggcatc ctggcaaggc aagtccgcac tgatgtgcga cgttccaacg gctgatgcat
    14101 agaccagtcg ccagctcaca ctgacgtcca gccttttggc gcttccagca caacttccgt
    14161 tcccggcgtg gagcgggaaa ccgaacgcag cgaactggat aaccagctaa acagttcacg
    14221 gaattgtaat ccctgcagtg gcaaaggctg acgaacgctg atttgtgcca gcgtcttcat
    14281 atccgccccc tgaacgccga tggtaaagaa ggcaaacttc ttatcttctt ccccctgaaa
    14341 cactttgttg gcggcagcct gccactcatc ggttggtgca ccatcggtga tcaggaaaat
    14401 ccacggacga taataggaaa taccattggc acgatattca cgtttccgct cctcaaccat
    14461 atccagggct ttggtaatgg cagagcccat tggtgtatcg ccctgggcaa acaggatggg
    14521 cgggaaaaaa ttagcggcgc tggtaaaggg ttgttccaca tgcaccgggc cgaacgtcac
    14581 aatgccgagt tccactcttt ttaaggccag cgaatcggca agcagctcat cgcgaaaggt
    14641 aaccaatccg gcgttaagtt cattaatggg tctaccgttc atagaaccag aaacatccag
    14701 cagcaaaatg caggggcaac gtggttccgg gttactggca aaatcgctgg tggcaaacgt
    14761 gatttgttcg ctcataagaa atcctttctt agagaattat tgaggtccgg tatcataccg
    14821 actgcaataa tgaaataaga gcaaacgaag aaactttcag aattaggccc ggcggcgcag
    14881 aagattttgc tattcgcctc gcaagaacgt gggcttacag attgacaaca agggttgagt
    14941 gacataattt caggagtgag ggtagagcgg ggtttccccc gccctggtag tcttagtatg
    15001 ctgggtagct aatgactaag agcaccacga tgatgagtag cttcatcatg accctttcct
    15061 tatttaaggc cccttcctcg ggaggggctt tcccgtttca gcgtcccgct gaaatcttcg
    15121 gcttacctcc tttcgccctg tatgcagtct atcgctgact tgcggataaa atagtttcaa
    15181 gtgtgattat tgcgtaacga ctcgcaaaga atatttgctg gcaggatcgc agactgcaaa
    15241 gtctgcggat tgacaattgg accgtgaagg catactttca ggaatgaggg ttgagcgggg
    15301 tttcccccgc cctggtagtc ttagtaagcc gggaagctta tgactaagag caccacgatg
    15361 atgatccact tcatcatgac cctttcctta tttatggccc cttcctcggg aggggctttc
    15421 ccgtttcagc gtcccgctga aatcgtcggc ttatctcctt tcgccatgca agcagtctat
    15481 cgctaacgcg tagataaaat agtttcatgt gttattactg gatgcgtgct cgcaaaagtg
    15541 cccgtcattc agacgattcc cgacagtgtt tcataattcc tccatttttc tcccttattg
    15601 gctggctaca ctagtatcat tccgcgaaac gtttcaggaa gagaaactct taacgatgaa
    15661 aggcagttat aaatcccgtt gggtaatcgt aatcgtggtg gttatcgccg ccatcgccgc
    15721 attctggttc tggcaaggcc gcaatgactc ccagagtgca gcccctgggg cgacgaaaca
    15781 agcgcagcaa tcgccagcgg gtggtcgccg tggtatgcgt gccggcccat tagctcctgt
    15841 tcaggcggcg accgcagtag aacaggctgt tccacgttac ctcaccgggc ttggcaccat
    15901 taccgccgct aatactgtta cggtgcgcag ccgcgtggac ggtcaactga tggcgttaca
    15961 tttccaggaa ggccagcagg tcaaagccgg cgatttactg gcagaaattg acccaagcca
    16021 gttcaaagtt gcattagcac aagcccaggg ccaactggca aaagataaag ccacgctcgc
    16081 caacgcccgc cgtgacctgg cgcgttatca acaactggca aaaaccaatc tcgtatctcg
    16141 tcaggaactg gatgcccaac aagcgctggt cagtgaaacc gaaggcacca ttaaggctga
    16201 tgaagcaagc gtcgccagcg cgcagttgca actcgactgg agccgtatca ccgcaccagt
    16261 tgatggtcgc gttggtctca agcaggttga tgttggtaac caaatctcca gtggcgacac
    16321 caccgggatt gtggtgatca cccaaacaca tcctatcgat ctggtcttta ctctgccgga
    16381 aagcgatatc gccaccgttg tgcaggcgca aaaagccgga aaaccgctgg tggtagaagc
    16441 ctgggatcgc accaactcga agaaattaag tgaaggcaca ctgttaagcc ttgataacca
    16501 aatcgatgcc actaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc
    16561 gctgtttccc aatcagtttg ttaacgcacg catgttagtc gacaccgaac aaaacgccgt
    16621 ggtgatccca accgccgccc tgcaaatggg caacgaaggc cattttgtct gggtgctgaa
    16681 tagcgaaaac aaggtcagca aacatctggt gacaccaggc attcaggaca gtcagaaagt
    16741 ggtgatccgc gcaggtattt ctgcgggcga ccgcgtagtg acggacggaa ttgatcgcct
    16801 gaccgaaggg gcgaaagtgg aagtggtgga agcccagagc gccactactc cggaagaaaa
    16861 agccgccagc cgcgaagaca cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg
    16921 agcagcacag gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg
    16981 atggtggcga tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg
    17041 ccggaagtgg actatccaac cattcaggtg gtcacgcttt atccaggtgc aagcccggat
    17101 gtcatgacct ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg
    17161 aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta tcactttaca gttccagcta
    17221 acattgccgc tggatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac
    17281 ctgctaccga gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatcca
    17341 ccgatcatga cgctcgctgt cacctcaacc gccatgccga tgacgcaggt ggaagatatg
    17401 gtggaaaccc gcgtcgcgca gaaaatctcg cagatttccg gcgtagggct ggtgacactt
    17461 tccggcgggc agcgtccggc ggtacgcgta aaactcaacg ctcaggcaat tgccgctctc
    17521 ggcctgacca gcgaaagtgt gcgcaccgcc attactggcg cgaacgttaa ctcagcgaaa
    17581 gggagccttg acggcccttc tcgcgcggtc acgctgtcag cgaatgacca gatgcaatcc
    17641 gccgaagagt atcgccagct aatcatcgcc taccagaacg gcgcaccaat tcgtcttggc
    17701 gatgtcgcaa ccgtagagca aggagcagaa aacagctggc tcggcgcgtg ggcgaacaaa
    17761 gaacaggcca ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc
    17821 gacagcatcc ggcagatgct accacagctc accgagagtc tgccgaaatc ggtgaaggtg
    17881 acggtacttt ctgaccgtac caccaatatc cgcgcatcgg tcgacgatac ccagtttgag
    17941 ctgatgatgg ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg
    18001 gcgaccatca ttcccggtgt tgctgtaccg ctgtcgttaa ttggcacctt tgcggtcatg
    18061 gtgtttctcg atttttcaat caataacctg acgttgatgg cgttaactat cgccaccgga
    18121 ttcgtggtcg atgacgccat cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc
    18181 gaaaaaccgc tggcagccgc gctcaaaggc gcgggtgaaa tcggctttac cattatctcg
    18241 ctgaccttct cgctgattgc ggtattgatc cccctgctgt ttatgggcga tatcgtcggg
    18301 cgactgttcc gcgaatttgc tattaccctg gcagtcgcga ttttgatctc agcagtggtg
    18361 tcgctgaccc tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gctgcgtaaa
    18421 cagaaccgct tctcccgtgc ctcggagaaa atgttcgaca ggataatcgc cgcctatggt
    18481 cgtggactgg cgaaagtgct gaatcatccg tggctgacct taagcgtagc gctcagcacg
    18541 ctgctgctta gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac
    18601 aacggcataa ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc
    18661 cagcggcaac gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagtttgacc
    18721 tcatttgttg gcgttgatgg cactaacccg tcgctgaaca gtgcgcgttt acagatcaat
    18781 ctcaaaccac tggatgaacg agatgaccgg gtgcaaaaag tcatcgcccg tctgcaaacg
    18841 gcggtggata aagtgccggg cgtagatctc ttcctgcaac caacgcagga cctgactatt
    18901 gatactcagg tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg
    18961 ctcagtacct gggtgccaca gttgatggaa aaactccagc agctgccaca gctttctgat
    19021 gtctccagcg actggcagga caaagggctg gtggcgtatg tcaatgtcga cagggacagc
    19081 gccagccgtc tggggatcag catggcggat gtcgataacg ccctgtacaa cgcgtttggt
    19141 cagcgactga tttccactat ttatactcag gctaaccagt atcgcgtggt gctggaacac
    19201 aacaccgaaa ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac
    19261 ggtggcgtgg tgccgttaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc
    19321 atcaaccatc tggatcagtt cccggtaacg actatctcct ttaacgtgcc ggataactat
    19381 tcgctgggcg atgcggtgca ggcgattatg gacacagaga agacgctgaa tctgccggtg
    19441 gatatcacca cgcagttcca gggcagcacc ctcgccttcc agtcggcgct gggcagcact
    19501 gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag
    19561 agctttattc acccgatcac cattctctcg acgctaccca ccgcaggggt cggcgcgctg
    19621 ctggcgttaa tgattgccgg tagcgaactg gatgtgattg ccattatcgg cattattttg
    19681 ctgatcggta tcgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag
    19741 cgcgagcaag gcatgtcgcc gcgcgaagct atttaccagg cctgtctgtt gcgttttcgt
    19801 ccgatcctga tgaccactct ggcggctctg cttggtgcgc tgccgctgat gttgagtacc
    19861 ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc
    19921 agccaggtgc tgacgctgtt taccacgccg gtgatttatc tgttgttcga ccgtctggca
    19981 ttgtggacca aaagccgttt tgcccgtcat gaagaggagg cgtaagtgaa gttttttgcc
    20041 ctcttcattt accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc
    20101 atactgggtt tccgtatgct gccagtcgcc ccgctgccgc aggtcgattt tccggtgatc
    20161 atggtcagcg cctcgctgcc cggtgcgtca ccagaaacaa tggcgtcttc cgttgccacg
    20221 ccgctggagc gctcacttgg gcgcattgcc ggagtcagcg aaatgacctc cagcagttcg
    20281 ctcggcagca cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg
    20341 cgcgatgtgc aggcggcgat caacgccgcg caaagtctgc tacccagcgg gatgcccagc
    20401 cgtccgacct atcgcaaagc gaacccatcg gatgcgccaa ttatgatcct cacgctgaca
    20461 tccgatactt attcgcaggg tgaactgtac gatttcgcct cgacacagct ggctccgacg
    20521 atttcgcaaa tcgacggtgt cggtgatgtc gatgttggcg gcagctcgct gcccgccgta
    20581 cgcgtcgggc tgaatccgca ggcgctgttt aatcagggcg tctcgctgga cgacgtacgc
    20641 accgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggtactcac
    20701 cgctggcaga tccagactaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt
    20761 attcattaca acaacggcgg agcggttcgt ctgggcgatg tggcgacggt gaccgactca
    20821 gtacaggatg tgcgcaacgc cgggatgacc aacgccaaac cggctatttt gctgatgatc
    20881 cgcaagctgc cggaagccaa tattatccag acggtagaca gcatccgcgc ccgtctgccg
    20941 gaattacaat ccaccatccc ggcagcaatt gatctgcaaa ttgcccaaga tcgctcccct
    21001 actatccgcg cgtcgctgga agaggttgag cagacgctgg taatctcagt tgcactggtg
    21061 attctggtgg tgtttttgtt cctgcgttcg ggtcgcgcca ccattattcc cgccgttgcg
    21121 gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc tgtgtggttt cagtctcaat
    21181 aatctttcgt taatggcgct cactatcgcc actggctttg tggtggatga cgccatcgtg
    21241 gtgctggaaa acattgcccg tcatctggaa gcggggatga aaccgctaca ggccgcgcta
    21301 caaggtactc gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg
    21361 ttcctgccgc tgctgttgat gggcggattg ccgggtcgcc tgttacgcga gtttgccgtg
    21421 acgctttctg tcgccattgg tatttcgctg ctggtttccc tgacgttaac gccgatgatg
    21481 tgcggctgga tgctaaaagc cagcaagccg cgcgagcaaa agcgactgcg tggctttggt
    21541 cgcatgttgg tagccctgca acaaggctat ggcaagtcgc tgaaatgggt gctcaatcat
    21601 acccgtctgg tgggcgtggt gctgcttggc accattgcgc tgaatatctg gctgtatatc
    21661 tcgatcccga aaaccttctt cccggagcag gacaccggcg tactgatggg tgggatacag
    21721 gctgaccaga gtatttcgtt tcaggcgatg cgcggtaagt tgcaggactt catgaaaatt
    21781 atccgtgacg atccggcggt ggataatgtc accggcttta ccggcggttc acgggtgaac
    21841 agtggaatga tgtttatcac ccttaaacca cgcggcgagc ggagcgaaac ggcccaacag
    21901 attatcgatc gcttgcgtaa aaaactggct aaagaaccgg gagcgaatct gttcctgatg
    21961 gcggtacagg atattcgcgt tggcggacgt caggctaacg ccagttacca gtacacgttg
    22021 ttatccgacg acctggcggc actgcgcgaa tgggagccga aaatccgcaa aaaactggcg
    22081 acattgccag agctggctga tgttaactcc gatcaggaag ataatggcgc ggagatgaac
    22141 cttatttacg atcgcgacac catggcgcga ttgggaattg acgtgcaagc cgccaacagc
    22201 ctgttaaata acgccttcgg tcagcggcaa atctcgacca tttaccagcc gatgaaccag
    22261 tacaaagtgg tgatggaagt ggatccgcgc tatacccagg acatcagtgc actggaaaaa
    22321 atgttcgtta tcaataacga aggaaaagcg atcccgctgt cgtatttcgc taaatggcaa
    22381 ccggcgaacg ctccactgtc ggtgaatcat cagggattat cggcggcctc gaccatttcg
    22441 ttcaacctgc cgaccgggaa atcgctctcg gacgccagtg cggcgatcga tcgcgcaatg
    22501 acccagcttg gtgtgccttc gacggtgcgc ggcagttttg ccggaacagc gcaggtgttc
    22561 caggagacga tgaactcaca ggtgatcctg attatcgccg ccatcgccac ggtatatatc
    22621 gtactgggta tcctttacga gagttatgtg catccactga cgattctctc caccctacct
    22681 tcggcgggcg tcggagcgct gttggcgctg gagctgttca atgccccgtt cagcctaatt
    22741 gcgctgatag ggatcatgtt attaatcggc atcgtgaaga aaaacgccat tatgatggtc
    22801 gattttgcgc ttgaagccca acggcacggt aacctgacgc cgcaggaagc tattttccag
    22861 gcctgtctgc tgcgttttcg cccgattatg atgactaccc tggcggcgct gtttggtgcg
    22921 ctgccgctgg tattgtcggg cggtgacggc tcggagttgc ggcaaccatt ggggatcacc
    22981 attgtcggcg gtctggtcat gagccagctt ctgacgctgt ataccacgcc ggtggtgtat
    23041 ctctttttcg accgtctgcg gatgcgtttt tcgcgtaaac ctaaacaagc ggtaaccgag
    23101 taaatgacag atcttcccga cagcacccgc tggcaattgt ggattgtggc tttcggcttc
    23161 tttatgcagt cgctggacac caccatcgtt aacaccgcgc ttccctcaat ggcgcaaagc
    23221 ctcggggaaa gtccgttgca tatgcacatg gtcattgtct cttatgtact gaccgtggcg
    23281 gtgatgctgc ccgccagcgg ctggctggcg gacaaagtcg gcgtgcgcaa tattttcttt
    23341 accgccatcg tgctgtttac tctcggttca ctgttttgcg cgctttccgg cacgctgaac
    23401 gaactgttgc tggcacgcgc gttacagggc gttggcggcg cgatgatggt gccggtcggc
    23461 aggttgacgg tgatgaaaat cgtaccgcgc gagcaatata tggcggcgat gacctttgtc
    23521 acgctgcccg gtcaggtcgg gccgctgctt ggcccggcgc tcggcggtct gctggtggag
    23581 tacgcatcgt ggcactggat ctttttgatc aacattccgg tgggaattat tggcgcgatc
    23641 gccacactga tgttaatgcc gaactacacc atgcagacgc ggcgctttga tctctccggc
    23701 tttctgttgc tggcggttgg catggcggta ttaaccctgg cgctggacgg cagtaaaggt
    23761 acaggtttat cgccgctggc gattgctggc ctggtcgcag ttggcgtggt agcactggtg
    23821 ctttatctgc tgcacgccca aaataacaac cgcgccctgt tcagtctgaa actgttccgc
    23881 actcgtacct tttcgctggg cctggcgggg agctttgccg gacgtattgg cagtggcatg
    23941 ttgcccttta tgacaccggt tttcctgcaa attggcctcg gtttctcgcc gtttcatgcc
    24001 ggactgatga tgatcccgat ggtactcggc agcatgggaa tgaagcgaat tgtggtacag
    24061 gtagtgaatc gctttggtta tcgtcgggta ctggtggcga ccacgctggg cctgtcgctg
    24121 gtcaccctgt tgtttatgac taccgctctg ctgggctggt actacgtttt gccgttcgtc
    24181 ctgtttttac aaggaatggt caactcgaca cgtttctcct ccatgaacac cctgacgctg
    24241 aaagatctcc cggacaacct ggcgagcagc ggcaacagtc tgctgtcgat gattatgcaa
    24301 ttgtcgatga gtattggcgt caccatcgcc gggctgttgc tgggactttt tggttcgcag
    24361 catgtcagcg tcgacagcgg caccacacaa accgtcttta tgtacacctg gcttagcatg
    24421 gcgtctatca tcgcccttcc ggcgttcatc tttgccagag tgccgaacga tacgcatcaa
    24481 aatgtggcta tttcacggcg aaaaaggagc gcgcaatgaa gttctggcga cccggtatta
    24541 ccggcaaact gtttctggcg attttcgcca cctgcattgt cttattgatc acgatgcact
    24601 gggcggtacg tatcagtttt gagcgcggct ttatcgatta catcaagcat ggtaatgaac
    24661 aacggctgca aatgctcggc gatgcgcttg gtgagcagta cgcccagcac gggaactggc
    24721 gcttcctgcg taataacgat cgctttgtat ttcagatcct acgttcactg gagcatgata
    24781 acaacgaaga taagcccggc cccggtatgc cgccacacgg ctggcgcaca caattttggg
    24841 tggttgatca aaacaacaaa gtgctggttg gcccgcgagc accagttcca cccgacggca
    24901 cacggcggcc cattatggtc aatggtgcgg aagttggcgc ggtgatcgcc tcccctgttg
    24961 aacgactgac ccgtaatact gatatcaatt ttgacagaca acagcggcaa accagttggc
    25021 tgattgtggc tttatctacc ttattagcgg cgctggcgac attcccactg gcgcgcggtt
    25081 tgctggctcc ggtaaaacga ctggtggatg gcacgcacaa actggcagcg ggtgatttca
    25141 ctactcgcgt agcccccacc agcgaagatg agttgggcag actggcagag gatttcaatc
    25201 aactcgccag cacactggag aaaaaccagc agatgcgccg cgattttatg gccgatattt
    25261 ctcatgaact gcgcacgcct ttagcagtac tgcgcggcga actggaagcc attcaggatg
    25321 gtgtgcgtaa attcacgccg gagacgctgg cttctttaca ggcagaagta ggtacactga
    25381 caaaactggt tgacgatctc catcaattgt ctatgtctga tgaaggcgct ctcgcctatc
    25441 aaaaagcacc gatagatttg atcccactgc tggaagtggc gggaggcgca tttcgcgaac
    25501 gattcgccag ccgtggcctg aaattgcaat tttccctgcc agacagtatt accgtatttg
    25561 gcgatcgcga tcgtttaatg cagttgttca ataacttact ggaaaacagc ctgcgctaca
    25621 ctgacagcgg cggcagcctg aaaatctctg ccgagcagca cgacaaaacg gtgcgcctga
    25681 cctttgccga cagtgcgccg ggtgtcagtg acgatcagct acaaaaattg tttgaacgtt
    25741 tttatcgcac cgaaggttcc cgcaaccgcg ccagcggtgg ttccgggctg gggctggcga
    25801 tttgcctgaa cattgttgaa gcacataacg gccgcattat tgccgcccat tcgccttttg
    25861 gcgggcttag cattacagta gagttaccgc tggaacggga tttacagaga gaagtatgac
    25921 cgagttacca atcgacgaaa acacaccgcg tattttgatc gtggaagatg aaccgaagct
    25981 ggggcagttg ctcattgatt atctgcgtgc tgcgagctat gcgccaacgt tgattagcca
    26041 cggcgatcag gtactgccat atgtgcgcca gacaccaccg gatctgatcc tgttggatct
    26101 gatgctccct ggcaccgatg gcctgacgct gtgccgggaa attcgtcgtt tttctgacat
    26161 tccgatcgtg atggtgacgg caaaaatcga agagatcgac cgcctgctgg ggttggagat
    26221 tggcgcagat gactatatct gcaagccgta cagcccacgg gaagtggtag cgcgcgtcaa
    26281 aaccattttg cgccgttgca aaccgcagcg tgagttgcag caacaggatg ctgaaagccc
    26341 gttgattatc gacgaaggtc gttttcaggc ttcctggcgc ggtaaaatgc ttgacctgac
    26401 gcctgcagaa tttcgactgc tgaaaacgct ctctcatgaa ccaggaaaag tgttctcccg
    26461 cgagcagttg ctgaaccatc tttatgacga ctaccgcgtg gtaaccgacc gtaccatcga
    26521 cagccacatt aaaaacctgc gccgcaagct ggaatctctc gacgccgaac agtcatttat
    26581 ccgcgccgtt tacggcgtcg gttaccgctg ggaagccgac gcctgccgca tcgtttaaca
    26641 tttttacctg ccggatctgg ccttcagacc agatccggca acattatccc acgcatagtc
    26701 agcaaactga catgatctct ccgccctggc aggcaccaga aaatcgctca tactttaatc
    26761 ggcaaacagt ccttttagat gctgttttcg atacgcaatt tcaatcaagg agtcattatg
    26821 gctggttggt ttgaactcag taagagcagt gataatcaat tccggtttgt gctaaaagcg
    26881 ggcaatggtg agactatcct caccagcgag ctgtatacct caaaagcctc tgcggaaaag
    26941 ggcatcgcgt cggtacgtag caacagcccg caagaagaac gctatgagaa aaaaacggca
    27001 agcaacggca aattctattt caatctgaaa gccgccaatc atcaaattat cggctccagc
    27061 cagatgtacg ccaccgcgca atctcgtgaa accggaattg cctccgttaa agcaaatggc
    27121 acaagccaga cggtgaaaga caatacgtaa ttacgatgcc gggcgtaggt tgttggcgtc
    27181 cggcgcttta ccagttacct ccacagcgct acaatgcccg cccttaaagt gggggcactc
    27241 ccctgaccgc ttcatcaggt gaagcggatc tgacctgtca tcagaacgag agaattatgt
    27301 ttaaaccgga actcctttcc ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt
    27361 atggcgcaga tgctgtttat gcgggccagc cgcgttactc cctgcgtgtg cgcaacaacg
    27421 aattcaacca cgaaaatctt caactcggca ttaacgaagc ccacgcgctg gggaaaaagt
    27481 tttatgtcgt ggtcaacatt gcaccgcaca acgccaagct gaaaaccttt atccgtgacc
    27541 tgaaaccggt ggtggaaatg gggccggatg cgctgattat gtccgatcca gggctgatta
    27601 tgctggtgcg tgagcacttc cctgaaatgc cgatccatct ctcggtacag gctaacgccg
    27661 tgaactgggc gacggtgaaa ttctggcagc aaatgggcct gacccgcgtg atcctctctc
    27721 gcgaactgtc actggaagag attgaagaga tccgcaatca ggtgccggat atggaaatcg
    27781 aaatcttcgt tcacggcgcg ctgtgcatgg cctactccgg tcgctgcctg ctctctggct
    27841 atatcaacaa gcgcgacccg aaccagggca cctgcaccaa cgcctgccgc tgggagtaca
    27901 acgtccagga agggaaagaa gatgacgttg gcaacatcgt acacaagtac gagccgattc
    27961 cggtgcaaaa tgttgagccg acgctgggta tcggcgcgcc aaccgacaaa gtgtttatga
    28021 tcgaagaagc ccagcgtccg ggcgagtata tgaccgcgtt tgaagatgag cacggcactt
    28081 acatcatgaa ctcgaaagat ctgcgcgcca tcgcccatgt tgaacgcctg accaaaatgg
    28141 gcgtgcattc gctgaaaatc gaaggccgta ccaaatcttt ctactattgc gcacgcaccg
    28201 cgcaggttta ccgtaaagct atcgatgacg ccgctgcggg caaaccgttc gataccagcc
    28261 tgctggaaac tctggaaggt ctggcgcatc gtggctatac cgaaggtttc ctgcgtcgtc
    28321 atactcacga cgattatcag aactacgaat acggttattc agtttctgac cgccagcagt
    28381 ttgttggtga gtttaccggt gagcgcaagg gggacctcgc ggcggtagcg gtgaaaaata
    28441 aattctccgt tggcgacagc cttgagctaa tgacgccgca aggcaacatt aactttaccc
    28501 ttgagcacat ggaaaacgcc aaaggtgaag ctatgccggt cgcaccaggc gatggttata
    28561 ctgtgtggct cccggtgccg caggatcttg agctaaatta cgcgctgctg atgcgtaatt
    28621 tctccgggga aaccacgcgt aacccccacg gtaagtgatt aatttcgatt atttttcccg
    28681 gatggaaaat tcttagaaac cgatcacata cagctgcatt tattaaggtt atcatccgtt
    28741 tcgctgaaaa acataaccca taaaatgcta gctgtaccag gaaccacctc cttagcctgt
    28801 gtaatctccc ttacacaggc ttatttttta tgcataagcc ctatccctgg tcaccgtctt
    28861 ccattgacca catcgataga atctccctta atagcacgat gcctttcact tatcgacatc
    28921 gtgctcgcac aggttccggt tacgcacagc cagaacgcgc atgtttgacg cttaccaaaa
    28981 aatgaaccta aagcattgga atatttttga catcattttc tgatggctgc ataaaataaa
    29041 aattctgctt tagtttcatc tatctgtttg tcattattac tcacattcaa taatggtgtt
    29101 gaagaatatc ccatcacaga taaaataaat atgtgcatgg tagtcttgaa tctattctca
    29161 ctctccacat ttgaatgtca gacgagcaac gccatgtaat cctgcacctt ctgtcttcaa
    29221 tttaactatc tgcatttttt tgccctgagt aacacagaaa tgggctgcat cattttttac
    29281 tatattttct gcaccagata ttctgcccct ggctaaagaa gcttcggctt cggtgtagt
//
LOCUS       DACADF010000050        29161 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.050, whole genome shotgun sequence.
ACCESSION   DACADF010000050 DACADF010000000
VERSION     DACADF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.050"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(177..1652)
                     /gene="ampG"
                     /locus_tag="ISB30_002080"
     CDS             complement(177..1652)
                     /gene="ampG"
                     /locus_tag="ISB30_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAP1989711.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1696..2274)
                     /locus_tag="ISB30_002081"
     CDS             complement(1696..2274)
                     /locus_tag="ISB30_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP1989712.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2579..2896
                     /gene="bolA"
                     /locus_tag="ISB30_002082"
     CDS             2579..2896
                     /gene="bolA"
                     /locus_tag="ISB30_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAP1989713.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3240..4538
                     /gene="tig"
                     /locus_tag="ISB30_002083"
     CDS             3240..4538
                     /gene="tig"
                     /locus_tag="ISB30_002083"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP1989714.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTEEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4784..5407
                     /gene="clpP"
                     /locus_tag="ISB30_002084"
     CDS             4784..5407
                     /gene="clpP"
                     /locus_tag="ISB30_002084"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP1989715.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5533..6807
                     /gene="clpX"
                     /locus_tag="ISB30_002085"
     CDS             5533..6807
                     /gene="clpX"
                     /locus_tag="ISB30_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAP1989716.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVIANRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6995..9349
                     /gene="lon"
                     /locus_tag="ISB30_002086"
     CDS             6995..9349
                     /gene="lon"
                     /locus_tag="ISB30_002086"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAP1989717.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9558..9830
                     /gene="hupB"
                     /locus_tag="ISB30_002087"
     CDS             9558..9830
                     /gene="hupB"
                     /locus_tag="ISB30_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAP1989718.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10022..11893
                     /gene="ppiD"
                     /locus_tag="ISB30_002088"
     CDS             10022..11893
                     /gene="ppiD"
                     /locus_tag="ISB30_002088"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP1989719.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREAAIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12044..12415
                     /locus_tag="ISB30_002089"
     CDS             12044..12415
                     /locus_tag="ISB30_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989720.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNMAVLTL"
     gene            12533..12931
                     /locus_tag="ISB30_002090"
     CDS             12533..12931
                     /locus_tag="ISB30_002090"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAP1989721.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12983..13678)
                     /gene="queC"
                     /locus_tag="ISB30_002091"
     CDS             complement(12983..13678)
                     /gene="queC"
                     /locus_tag="ISB30_002091"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAP1989722.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(13743..15443)
                     /locus_tag="ISB30_002092"
     CDS             complement(13743..15443)
                     /locus_tag="ISB30_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAP1989723.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTVL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSLRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15543..16361
                     /gene="cof"
                     /locus_tag="ISB30_002093"
     CDS             15543..16361
                     /gene="cof"
                     /locus_tag="ISB30_002093"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAP1989724.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16514..16972
                     /locus_tag="ISB30_002094"
     CDS             16514..16972
                     /locus_tag="ISB30_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP1989725.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            17002..18774
                     /locus_tag="ISB30_002095"
     CDS             17002..18774
                     /locus_tag="ISB30_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAP1989726.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLVQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            18767..20548
                     /locus_tag="ISB30_002096"
     CDS             18767..20548
                     /locus_tag="ISB30_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAP1989727.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     SLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTLLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20729..21067
                     /gene="glnK"
                     /locus_tag="ISB30_002097"
     CDS             20729..21067
                     /gene="glnK"
                     /locus_tag="ISB30_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP1989728.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21097..22383
                     /gene="amtB"
                     /locus_tag="ISB30_002098"
     CDS             21097..22383
                     /gene="amtB"
                     /locus_tag="ISB30_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAP1989729.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22432..23292)
                     /gene="tesB"
                     /locus_tag="ISB30_002099"
     CDS             complement(22432..23292)
                     /gene="tesB"
                     /locus_tag="ISB30_002099"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAP1989730.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23510..24082
                     /locus_tag="ISB30_002100"
     CDS             23510..24082
                     /locus_tag="ISB30_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989731.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24113..24424)
                     /locus_tag="ISB30_002101"
     CDS             complement(24113..24424)
                     /locus_tag="ISB30_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAP1989732.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            24587..24683
                     /gene="ffs"
                     /locus_tag="ISB30_002102"
     ncRNA           24587..24683
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ISB30_002102"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24803..25156
                     /locus_tag="ISB30_002103"
     CDS             24803..25156
                     /locus_tag="ISB30_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HAP1989733.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DIPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25198..26748)
                     /gene="pdeB"
                     /locus_tag="ISB30_002104"
     CDS             complement(25198..26748)
                     /gene="pdeB"
                     /locus_tag="ISB30_002104"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAP1989734.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDELAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWYRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26912..27382)
                     /locus_tag="ISB30_002105"
     CDS             complement(26912..27382)
                     /locus_tag="ISB30_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989735.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27498..28049)
                     /gene="maa"
                     /locus_tag="ISB30_002106"
     CDS             complement(27498..28049)
                     /gene="maa"
                     /locus_tag="ISB30_002106"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAP1989736.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28223..28441)
                     /gene="hha"
                     /locus_tag="ISB30_002107"
     CDS             complement(28223..28441)
                     /gene="hha"
                     /locus_tag="ISB30_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAP1989737.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28467..28841)
                     /gene="tomB"
                     /locus_tag="ISB30_002108"
     CDS             complement(28467..28841)
                     /gene="tomB"
                     /locus_tag="ISB30_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAP1989738.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         7360 a         6983 c         7857 g         6961 t
ORIGIN      
        1 gagaaaataa ggactcatca gctgaatcgt gtaaaaaaaa tagaattcat aaatggcgtc
       61 aataatttcc agacgctaag ccgcacaaaa gaacaaatat caataagcga tgctgatgtt
      121 tatttagccg taataattac ggcgagtgat tactacagct aaataatatt tacagattac
      181 gtcagatgtg tttttcgtag cgccagataa tccagcaaac caccaagcac gacgccagag
      241 agggcgacta aaaccccgac ttccagcagc gcaggcagga aagagaagtg cgtcaaatcc
      301 agcgcgtcca tcgtcaacag cagtaaccac acggccaaca ggctgatgcc cgccgccagt
      361 gtccacatgg caaaggcata acctgcagga tattcggtac gggagataaa gttgtcattt
      421 actcgtgtat attcaagcgt ctggcggcaa accagcagca aaataagccc tggtacggca
      481 gcggcgacgg agaatagata gaatgtcgac cagccgtgtg cttcaacaaa ccaacccgcc
      541 acggggccga cataaactcg ccctacagca gaaagcgctg agagcagggc aaattgagta
      601 gcggaaaatg acttattaca cagcgtcatt aacagcgcga caaaggctga tgtacccatc
      661 ccgccacaga ggttttcgaa aaagacggct gcacccatgc tgtagagatg cttatcagta
      721 atcgacagca gccagtaacc ggcgttagat gcaccttgta aaatgccgaa aatcagcagt
      781 gcacggaaca gcgacaggcg ctgcatcaaa atcccaccgt acaatgcgcc aacaatggtc
      841 gccagtaagc caagcgtttt gttaaccacg cctacttcac ccgcatcaaa cccgacgccg
      901 cgaatcaaaa acgtggttgt caggctcata gcgaatgcgt cgcccagctt atacagcacg
      961 ataagaagca aaataagcca ggcattattg cgaccaaaga aatctcgcag aggtgcaaca
     1021 accgcttgtt ccagcgtttt gggcacagga atggtgtcgg tgggttctgg tgcaagcaac
     1081 gtcgcgataa tacaggggat caacagcgcc gccatcaacc agtacatgcc ctgccagccc
     1141 agccatttat ctgccagcca cagggctaag ccgccggaaa ccagcatccc taaacggtaa
     1201 cccagcacgc tgattgccgc gcccgcacca cgttcttctg ccgggagcac atcggttttc
     1261 cacgcatcga agacaatatc ctgggaggca gagcaaaaag cgatcaccac cgccagtgcc
     1321 gccatccagc ggagctgggt gcctggttcg agaaaaccca tcgccgcaat ggcgactaat
     1381 aacagaattt gcgtggcgag cagccaaccg cgccgccgcc cgaaaaatgg gggcgtgtag
     1441 cggtccatca gtggtgacca gaggaattta aaaacgtaag cctggcctac cagagagaag
     1501 aaaccaatgg ttttgagatc gatattctcg accgtcatcc aggcctgtaa ggtgccggac
     1561 gtcagggcga gcggtagccc ggaagcaaaa cccaggatca gcaatatggc tgaacgcggc
     1621 tgttgaaaaa tacgtaaata ttgactggac atgggcgtct acaggcccgg cttgcaccgg
     1681 gccagagggc agaaattaac gcgcgttctg cttgatgaat tcgtggatgc tggtgtcctg
     1741 agacatatca gcgatggtat cggtcagcac actgttaacc gcatcggcga tatttttgtt
     1801 ggaggcctgg aacgcacctt caacgttgta gctggcacga tagtttttgg tcattttgtt
     1861 gccattctgc gcggtagcga tgatggcgat atccgctttg gtcgcgatgt tgtagcgcac
     1921 gttgccctgg gacacgtcag catacagttg gctaacgatg atttgcagat taaccgggcc
     1981 attcggacca accatgtatc cacgggcggt catttgtttt tccagcactt cttgcagcag
     2041 gaaacgcaga tcgcgggagg cggtcagggt aacgatttga ttatcgcggg tgacttttgc
     2101 cagcgcctga tcggtacgct gatcggcacc attaatgctt acggtgacgc ccatcaggct
     2161 tggatcctgc tgtggcagtg taatcgtcgg ggaaacttcg atagttgttg gcggttttgc
     2221 gcatcctgca agcataaaca gagcaactaa cgggaagagg atttttttga acatgttcgg
     2281 gctctcagag actcttaagc gtgtttggta aaaattcccg ccatcataac attgccaacg
     2341 gcgaggggaa gtgggtaagg catgtaaatt catcatgttg acgaaataat cacccctggt
     2401 aaaagaaacg ctgatgcgag gcctgtgttt caatctttaa atcagtaaac ttcatacgct
     2461 tgacggaaaa accaggacga aacctaaata tttgttgtta agctgcaatg gaaacggtaa
     2521 aagcggctag tatttaaagg gatggatgac atctcagcgt tgtcggagga gatatttcat
     2581 gatgatacgt gagcggatag aagaaaaatt aagggcggcg ttccaacccg tattcctcga
     2641 agtagtggat gaaagctatc gtcacaatgt cccagccggc tctgaaagcc attttaaagt
     2701 tgtgctggtc agcgatcgtt ttacgggtga acgttttctg aatcgtcatc gaatgattta
     2761 cagtacttta gcggaggaac tctctactac cgttcatgcg ctggctctgc atacttacac
     2821 tattaaggag tgggaagggt tgcaggacac cgtctttgcc tctcctccct gtcgtggagc
     2881 aggaagcatc gcgtaaaaac gcatttgcaa ctgtcggcgc ttttccagta tgttgctaaa
     2941 gattttatga aaaacggcct gcgggccgtt ttgttttgtc tggattttgc gctttttgcc
     3001 cagcattcag acgaaaatcg cccgggaatt gtgaaaaaat acgcgacagc gcgcaataac
     3061 cgttctcgac tcataaaagt gatgccgcta taatgccgcg tcctatttga atgctttcgg
     3121 gatgattctg gtaacaggga atgtgattga ttataagaac atcccggttc cgcgaagcca
     3181 acaacctgtg cttgcggggt aagagttgac cgagcactgt gattttttga ggtaacaaga
     3241 tgcaagtttc agttgaaacc actcaaggcc ttggccgccg tgtaacgatt actatcgctg
     3301 ctgacagcat cgagaccgct gttaaaagcg agctggtcaa cgttgcgaaa aaagtacgta
     3361 ttgacggctt ccgcaaaggc aaagtgccaa tgaatatcgt tgctcagcgt tatggcgcgt
     3421 ctgtacgcca ggacgtcctg ggcgacctga tgagccgtaa cttcattgac gccatcatta
     3481 aagaaaaaat caatccggct ggcgcaccga cctatgttcc gggcgaatac aagctgggtg
     3541 aagacttcac ctactctgta gagtttgaag tttatccgga agttgaactg cagggtctgg
     3601 aagcgatcga agttgaaaaa ccgattgttg aagtgaccga cgctgacgtt gacggcatgc
     3661 tggatactct gcgtaaacag caggcaacct ggaaagaaaa agacggcgct gttgaagcag
     3721 aagaccgtgt gaccatcgac ttcaccggtt ctgtagacgg agaagagttc gaaggcggta
     3781 aagcgtctga tttcgtactg gcgatgggtc agggtcgcat gatcccgggc tttgaagacg
     3841 gtatcaaagg ccacaaagct ggcgaagagt tcaccatcga cgtgaccttc ccggaagagt
     3901 accacgcaga aaacctgaaa ggtaaagcag cgaaattcgc tatcaacctg aagaaagttg
     3961 aagagcgtga actgccggaa ctgactgaag aattcatcaa acgtttcggc gttgaagatg
     4021 gttccgtaga aggtctgcgc gctgaagtgc gtaaaaacat ggagcgcgag ctgaagagcg
     4081 ccatccgtaa ccgcgttaag tctcaggcga tcgaaggtct ggtaaaagct aacgacatcg
     4141 atgtaccggc tgcgctgatc gacagcgaaa tcgacgttct gcgtcgccag gctgcacagc
     4201 gtttcggtgg caacgaaaaa caagctctgg aactgccgcg cgaactgttc gaagaacagg
     4261 ctaaacgccg cgtagttgtt ggcctgctgc tgggcgaagt tatccgcacc aacgagctga
     4321 aagctgacga agagcgcgtg aaaggcttga tcgaagagat ggcttctgcg tacgaagatc
     4381 cgaaagaagt tatcgagttc tacagcaaaa acaaagaact gatggacaac atgcgcaatg
     4441 ttgctctgga agaacaggct gttgaagctg tactggcgaa agcgaaagtg actgaaaaag
     4501 aaaccacttt caacgagctg atgaaccagc aggcgtaatt tacgtagcat aacgcgctaa
     4561 attcgcacaa aggcccgtca ccgccaggtg gtgggctttt ttttgtcatg aattttgcat
     4621 ggaaccgtgc gaaaagcctc tttcggtgtt agcgtaacaa caaaagattg ttatgcttga
     4681 aatatggtga tgccgtaccc ataacacagg gactagctga taatccgtcc ataaggttac
     4741 aatcggtaca gcaggttttt tcaattttat ccaggagacg gaaatgtcat acagcggcga
     4801 acgagataac tttgcacccc atatggcgct ggtgccgatg gtcattgaac agacctcacg
     4861 aggtgagcgc tcttttgata tctattctcg tctacttaag gaacgcgtca tttttctgac
     4921 tggccaggtt gaagaccaca tggctaacct gattgtggcg cagatgctgt tcctggaagc
     4981 agaaaaccca gaaaaagata tctatctgta cattaactct ccaggcgggg tgattactgc
     5041 cgggatgtct atctatgaca ccatgcagtt tatcaagcct gatgtcagca ccatctgtat
     5101 gggccaggcg gcctcgatgg gcgctttctt gctgactgca ggggcaaaag gtaaacgttt
     5161 ctgcctgccg aattcgcgcg tgatgattca ccagccgttg ggcggctacc agggccaggc
     5221 gaccgatatc gaaattcatg cccgtgaaat tctgaaagtt aaagggcgca tgaatgaact
     5281 tatggcgctt catacgggtc aatcattaga acagattgaa cgtgataccg agcgcgatcg
     5341 cttcctttct gcccctgaag cggtggaata cggtctggtc gattcgattc tgacccatcg
     5401 taattgatgc cagaggcgca actgtgccgc tatacttatc cagggcggca caacgctgta
     5461 agcggcttgc gcctgagaat ggcatttgcg tcgtcgtgtg cggcacaaag aacaaagaag
     5521 aggttttgac ccatgacaga taaacgcaaa gatggctcag gcaaattgct gtattgctct
     5581 ttttgcggca aaagccagca tgaagtgcgc aagctgattg ccggtccatc cgtgtatatc
     5641 tgcgacgaat gtgttgattt atgtaacgac atcattcgcg aagagattaa agaagttgca
     5701 ccgcatcgtg aacgcagtgc gctaccgacg ccgcatgaaa ttcgcaacca cctggacgat
     5761 tacgttatcg gtcaggaaca ggcgaaaaaa gtgctggcgg tcgcggtata caaccactac
     5821 aaacgtctgc gcaacggaga taccagcaat ggcgtcgagt tgggcaaaag taacattctg
     5881 ctgatcggtc cgaccggttc cggtaaaacg ctgctggccg aaacgctggc gcgcctgctg
     5941 gacgttccgt tcaccatggc tgacgcaacc acgctgaccg aagccggtta tgtgggcgaa
     6001 gacgttgaaa acatcattca gaagctgttg cagaagtgcg attacgacgt acagaaagcg
     6061 cagcgcggga ttgtctacat cgatgaaatc gacaagattt ctcgtaagtc agacaacccg
     6121 tccattaccc gagacgtttc cggtgaaggc gtacagcagg cactgttaaa actgatcgaa
     6181 ggtacggtag ctgctgttcc gccgcaaggt gggcgtaaac atccgcagca ggaattcttg
     6241 caggttgata cctctaagat cctgttcatc tgtggcggcg catttgctgg cctggataaa
     6301 gtgattgcca atcgtgttga aaccggctcc ggcattggtt ttggcgcgac ggtgaaagcc
     6361 aagtccgaca aagccagcga aggtgaactg ctggcgcagg ttgaaccgga agatctgatc
     6421 aagtttggtc tgatccctga gttcattggt cgtctgccgg ttgtcgcaac gttgaatgaa
     6481 ctgagcgaag aagctctgat tcagatcctc aaagagccga aaaacgccct gaccaagcag
     6541 tatcaggcgc tgtttaatct ggaaggcgtg gatctggaat tccgtgacga ggcgctggat
     6601 gctatcgcta agaaagcgat ggcgcgtaaa accggtgccc gtggcctgcg ttccatcgta
     6661 gaagccgcac tgctcgatac catgtacgat ctgccgtcca tggaagatgt cgaaaaagtg
     6721 gttatcgacg agtcggtaat tgatggtcaa agcaaaccgt tgctgattta tggtaagccg
     6781 gaagcgcaac aggcatctgg tgaataatta accattccca tacaattagt taaccgaaaa
     6841 ggggggattt tatctcccct ttaatttttc ctctattctc ggcgttgaat gtgggggaaa
     6901 catccccata tactgacgta catgttaata gatggcgtga agcacagtcg tgtcatctga
     6961 ttacctggcg gaaattaaac taagagagag ctctatgaat cctgagcgtt ctgaacgcat
     7021 tgaaatcccc gtattgccgc tgcgcgatgt ggtggtttat ccgcacatgg tcatcccctt
     7081 atttgtcggg cgggaaaaat ccatccgttg tctggaagcg gcgatggacc atgataaaaa
     7141 aattatgctg gtcgcgcaga aagaagcttc aacggatgag ccgggtgtaa acgatctttt
     7201 caccgtcggg accgtggcct ctatattgca gatgctgaaa ctgcctgacg gcaccgtcaa
     7261 agtgctggtc gaggggttac agcgcgcgcg tatttctgcg ctctctgaca atggcgaaca
     7321 cttttctgcg aaggcggagt atctggagtc gccgaccatt gatgagcggg aacaggaagt
     7381 gctggtgcgt actgcaatca gccagttcga aggctacatc aagctaaaca aaaaaatccc
     7441 accagaagtg ctgacgtcgc tgaatagcat cgacgatccg gcgcgtctgg cggataccat
     7501 tgctgcacat atgccgctga aactggctga caaacagtcc gttctggaga tgtccgacgt
     7561 taacgaacgt ctggaatatc tgatggcaat gatggaatcg gaaatcgatc tgctgcaggt
     7621 tgagaaacgc attcgcaacc gcgttaaaaa gcagatggag aaatcccagc gtgagtacta
     7681 tctgaacgag caaatgaaag ctattcagaa agaactcggt gaaatggacg acgcgccgga
     7741 cgaaaacgaa gccctgaagc gcaaaatcga cgcggcgaag atgccgaaag aggcaaaaga
     7801 gaaagcggaa gcagagctgc agaagctgaa aatgatgtct ccgatgtcgg cagaagcgac
     7861 cgtagtgcgt ggatatatcg actggatggt acaggtgccg tggaatgcgc gtagcaaggt
     7921 caaaaaagac ctgcgtcagg cgcaggaaat ccttgatacc gaccattatg gtctggagcg
     7981 cgtgaaagat cgtatccttg agtatcttgc ggttcaaagc cgtgtcaaca aaatcaaggg
     8041 tccgatcctc tgcctggtag ggccgccggg ggtaggtaaa acgtccctgg gtcagtccat
     8101 cgccaaagcc accgggcgta aatatgtccg tatggcgctg ggcggcgtgc gtgatgaagc
     8161 ggaaatccgt ggtcaccgcc gtacttacat cggttctatg ccgggtaaac tgatccagaa
     8221 aatggcgaaa gtgggcgtga aaaacccgct gttcctgctc gatgagatcg acaaaatgtc
     8281 ttctgacatg cgtggtgatc cggcctccgc actgcttgaa gtgctggatc cagagcagaa
     8341 cgtagcgttc agcgaccact acctggaagt ggattacgat ctcagcgacg taatgtttgt
     8401 cgctacgtcg aactccatga acattccggc accgttgctg gatcgtatgg aagtgattcg
     8461 cctctccggt tataccgaag atgaaaaact gaacatcgcc aaacgtcacc tgctgccgaa
     8521 gcagattgaa cgtaatgcat tgaaaaaagg tgagctgacc gtcgacgata gcgccattat
     8581 cggcattatt cgttactaca cccgtgaggc gggcgtgcgt ggtctggagc gtgaaatctc
     8641 caaactgtgt cgcaaagcgg ttaagcagtt actgctcgat aagtcattaa aacatatcga
     8701 aattaacggt gataacctgc atgactacct cggtgttcag cgtttcgact atgggcgcgc
     8761 ggataacgaa aaccgtgtcg gccaggtaac cggtctggcg tggacggaag tgggcggtga
     8821 cttgctgacc attgaaaccg cgtgcgttcc ggggaaaggc aaactgacct ataccggttc
     8881 gctcggcgaa gtgatgcagg agtctattca ggcggcgtta acggtggttc gtgcgcgtgc
     8941 ggaaaaactg gggatcaacc ctgattttta cgaaaaacgt gacatccacg tccacgtacc
     9001 ggaaggtgcg acgccgaaag atggtccgag tgccggtatt gccatgtgca ccgcgctggt
     9061 ttcttgcctg accggtaacc cggttcgtgc cgatgtggca atgaccggtg agatcactct
     9121 gcgtggtcag gtactgccga tcggtggttt gaaagaaaaa ctactggcag cgcatcgcgg
     9181 cgggattaaa acagtgttaa ttccgttcga aaataaacgc gatctggaag agattcctga
     9241 caacgtaatt gccgatctgg acattcatcc tgtgaagcgc attgaggaag ttctgactct
     9301 ggcgctacaa aatgaaccgt ctggcatgca ggttgtgact gcaaaatagt gacctcgcgc
     9361 aaaatgcact aataaaaaca gggctggcag gctaattcgg gcttgccagc ctttttttgt
     9421 ctcgctaagt tagatggcgg atcgggcttg cccttattaa ggggtgttgt aaggggatgg
     9481 ctggcctgat ataactgctg cgcgttcgta ccttgaagga ttcaagtgcg atataaatta
     9541 taaagaggaa gagaagagtg aataaatctc aattgatcga caagattgct gcaggggctg
     9601 atatctctaa agctgcggct ggccgtgcgt tagatgctat tattgcttcc gtaactgaat
     9661 ctctgaaaga aggggatgat gtagcactgg taggttttgg tacttttgcc gttaaggaac
     9721 gtgctgcccg tactggccgc aacccgcaga ccggtaaaga gatcaccatc gctgctgcta
     9781 aagtaccgag cttccgtgca ggtaaagcac tgaaagacgc ggtaaactaa gcgttgtccc
     9841 cagtggggat gtgacgaagt tcaagggcgc atctactgat gtgccttttt tatttgtatt
     9901 cggtgacttt ctgcgtcttg tgggctgaca attgcccccg tttcttgtca caataggcct
     9961 ttgcgcgcat cgatacgttg cgtgaggtac acagtcatct acagcggagt gttgttacac
    10021 catgatggac agcttacgca cggctgcaaa cagtctcgtg ctcaagatta ttttcggtat
    10081 cattatcgtg tcgttcatat tgaccggcgt gagtggttac ctgattggcg gaggcaataa
    10141 ctacgccgca aaagtgaatg accaggaaat cagccgtggg caattcgaga acgccttcaa
    10201 cagcgagcgt aatcgcatgc agcaacagct gggcgatcaa tactccgagc tggcagcgaa
    10261 cgaaggctat atgaaaaccc tgcgtcaaca ggtgctgaat cgtctgatcg acgaggcgct
    10321 tctggatcag tacgctcgtg agctgaaact gggtatcagc gatgagcagg ttaaacaggc
    10381 gattttcgcg accccagcct tccaggttga cggcaaattt gataacagcc gctataacgg
    10441 tatcctcaac cagatgggga tgaccgccga tcagtacgcc caggcgctgc gtaaccagct
    10501 cactacccaa cagctgatta acggcgttgc cggtaccgat tttatgctga aaggtgaaac
    10561 cgacgagctg gcggcactgg tagctcaaca acgcgtggtg cgtgaagcgg ctatcgatgt
    10621 taacgcgctg gcggcgaagc agcctgtgac cgaacaggaa attgccagct actacgaaca
    10681 aaacaaaaac aatttcatga cgccggaaca attccgcgtg agttacatca agctggatgc
    10741 cgcgacgatg cagcaaccgg ttagcgatgc ggatatccag agctactacg atcagcatca
    10801 ggatcaattc acccagccgc agcgtacccg ttacagcatc atccagacca aaactgaaga
    10861 tgaagcgaaa gcggtacttg atgagctgaa taagggcggt gattttgctg cattagccaa
    10921 agaaaaatct gccgatatta tctctgcccg taacggcggt gatatgggtt ggttagaaga
    10981 tgccactatc ccggacgaac tgaaaaatgc gggtctgaaa gaaaaaggcc aactgtctgg
    11041 tgttatcaaa tcttcggtcg gtttcctgat tgtacgtctg gacgacattc agccagcgaa
    11101 agtgaaatcg ttagacgaag tacgtgacga cattgcggcg aaagtgaaac acgaaaaagc
    11161 cctcgatgcg tactacgcgc tgcagcagaa agtgagcgat gcggcaagca acgacaccga
    11221 gtctctggcc ggtgcagagc aagctgccgg cgttaaagcc actcagacgg gttggttcag
    11281 caaagataac ctgccggaag agttgaactt caagccagtt gccgacgcta tcttcaacgg
    11341 cggtctggta ggtgaaaacg gcgcgccggg catcaactct gacatcatca ccgtagacgg
    11401 cgaccgcgca ttcgtgctgc gcatcagcga gcacaaaccg gaagcggtga aaccgttggc
    11461 agatgttcag gaacaagtta aggcactggt tcagcacaac aaagctgaac aacaggcgaa
    11521 agtggatgct gagaaactgc tggttgattt gaaagccggc aaaggtgcgg aagcgatgca
    11581 ggctgccggt ctgaaatttg gcgagccgaa aaccttaagc cgttccggtc gtgacccgat
    11641 tagccaggct gcgtttgcac tgccgctgcc agcgaaagac aaaccgagct acggtatggc
    11701 gaccgatatg caaggtaatg tggttctgct ggcgctggat gaagtgaaac aaggttcaat
    11761 gccggaagat cagaaaaaag cgatggtgca gggtatcacc cagaacaacg cacaaatcgt
    11821 ctttgaagct ctgatgagta acctgcgtaa agaggcgaaa atcaaaattg gcgatgcgct
    11881 ggaacagcaa taatcctgaa gccgcctcgc aaaaaaatgc tttgcagctg taacaagaaa
    11941 aggtcgcttt cgcggccttt tccatttctg aacattgcca tttgtttact gttttcactg
    12001 ccgttaaggt gattccactg ttaacaaaca aggagaaaac agtatgaaac acggaattaa
    12061 agcactgctc attaccctgt ccctggcctg tgccggaatg tctcatagtg ctctggcggc
    12121 agctcctgcg gcaaaaccga caacggtaga aaccaaagcg gaagctcctg cagcacaaag
    12181 taaagcagca gtaccggcga aagccagtga cgaagaaggc acccgggtca gcattaataa
    12241 tgccagcgcg gaagagttag cccgcgcgat gaatggcgtt ggcctgaaga aagcgcaggc
    12301 gattgtcagt tatcgcgaag agtacggtcc gtttaaaacg gtggaggatc taaagcaggt
    12361 gccggggatg ggcaattcgc tggtggaacg taatatggcg gtattaaccc tgtaattaat
    12421 ttgcatagtg gcaatttttt gccagactga agaggtcata ccagttatga cctctgtact
    12481 tataacagtt cttataacag ttcttataac aacaacgtaa ggttattgcg ctatgcaaac
    12541 acaaatcaaa gttcgtggat atcatctcga cgtttaccag cacgtaaaca acgcccgcta
    12601 ccttgaattt ctcgaagaag cccgctggca tgggttggaa aatagcgaca gttttcactg
    12661 gatgacggcc cataacatcg ctttcgtcgt ggtcaatatc aatattaact atcgccgccc
    12721 ggcggtatta agcgacctgt taaccattac cagtcagttg cagcaattaa acggtaaaag
    12781 tggcatctta agccaggtca ttacactgga gccggaagga caggtggtag cggatgcgct
    12841 tattacgttt gtttgtattg atcttaaaac gcagaaagca ttagctctgg aaggggaatt
    12901 gcgcgaaaag ctggagcaga tggttaagta aacgttttgt ggtgccggat gctcaagccg
    12961 catccggcga cacccggaat aattacttca acccggtttt ctgcttcatc gctgccatca
    13021 ccgtcggttt atcggccaga taatgattca aaccgttggc acgtaaatta cacgccgcac
    13081 aatgaccgca accgtcgcct ttaatgccgt tataacaggt cagcgtttcg ttacggacta
    13141 aatccagctt gccgtaataa tctgccagcg cccaggtttc cgctttatca atccacatca
    13201 gcggcgtttc aaaacgaata tctttcgcca tgcccaaact gacggcatgg tttagtgctt
    13261 tcacaaactc atcgcgacaa tccgggtagc cggagaaatc cgtttcgcag acgccggtaa
    13321 ttacggcttc tgcttttacc tgatacgcat atattgccgc cagcgtcagg aacaaaatat
    13381 tacgccctgg gacaaacgta ttcgggatac catcggcttc aggttcataa tcaggcaccg
    13441 gaatgctgtc acgcgtcagg ctgctgaccg ccagctcgtt gagcagcgtt acatcaagaa
    13501 ccttatgcgc gcgtgccccc agtttcagcg ccagttcgcg tgccacgtcg atttctgcgc
    13561 gatggcgttg accgtaatcg aacgtcacgc aatggacttc atcatattgt tgtaatgcct
    13621 gcaccagaca ggtggtggaa tcctggcctc cactgaacac aacgacagca cgtttcatag
    13681 ataatcctgc ttgaacaata aaagcgttat ggtaacgcct gcgattaacc cggaccagct
    13741 attcattgcg acggcgcggg aagccaggct tcggtaaatt caaaccagcc gcgcggtgtc
    13801 agtcgcacac cgttcacgcc gggaggggcg ctaatgcgat agtgatagtt gaacagcggc
    13861 gtcagcgtcg catctgccat taactggcta aaaaccgctt tcagggcatt aaatcggttt
    13921 tcctcatcag gcattacttg cactgcgtcc agtgtcgatt gcaaatgtgc atatgctgga
    13981 gcgtcgaaaa catgtggcca cagtggatcg caacgcagcc attgctccag ggtatattcc
    14041 ggtgcttcgc caattaatct gtcgcccatc atgaggtctg cgtgtgccag tagggtcgtg
    14101 tcgtcccagt tttttgcgtt atgaaaaata attgtgagtt cacagccttc cgctgccagt
    14161 gtcgcctgta ggcgttctgc catggtatga agttctatcg gtaggtgata aaccagcgtc
    14221 aaggttttcg gtagtttaac ttcatccggt acctgccaat gcggaatagt ccagccaggc
    14281 agtaatgcat gactggcggt gatcaggttt tctccgactt ctaacgtttg taataaaccg
    14341 gattgatgaa taatggagat tacttttcgc gcctgccaga gggacagtcg gagacttttg
    14401 cgcaatgtta aatagcaaaa acctaaactg atgccgctac tgacctggct gacccgttgc
    14461 agctcctccg gtttgccaat ggtgatttgc acgggatgcc gacaactggt tcccaaatct
    14521 ttttcgaaaa gcggcggagt tatccagtac tcaaccgctt taagcagcgg atgacgtaaa
    14581 tggtaataat catggctctc caggcgcacc agttccgctg tgaattgtgt taagcgaaaa
    14641 ggacctgtgc cgatcagtgg gaattgcgga tgcgccagat ggctgcaata gctcgccagt
    14701 cggtgcgcaa gccagtaatc ggggcgatgt aaaaagaagg tcagacactg cggatgggtg
    14761 acttcaatac gcttcacgct aataaataat tgatccagtg ctggcagttg taacagcatc
    14821 aataatcgct ggtgtaagtg tgaggctttt actgcatcgc cgttatgcca gtgtagcgtt
    14881 gaacgaagat aaaagtccca gcgtaacccg tcagtagagg tttcccagtg atgtgctaaa
    14941 tcgccaatcg ggcgctgagt attattatcg aagcgggtca ggccggaaaa tatctgcccg
    15001 gccagatgct gctcggcacg gccgggcaaa aagcccggtt gtagcggttc gagcgggcga
    15061 tagtagggaa tacgcaacgt gggcgtatcg ttttgccatt gtccgcccat aaacggctgt
    15121 aacagagtgc gcagctcacc tggggccagt tgcgccagct ccagcacatc ttgctgcttt
    15181 ccggtttcca gcgcctgttc catcatcgca ttgcgtagcg attctggcgt gaccagaaag
    15241 cgtagttgac cgcgctttcc gcgtcctgac tgcgcctgcc actccagcca tcccgcctcc
    15301 tgtgcctgac gcaacaccgt acgaacatgg cgttcgctgc aaaaacagcg ttcggccagt
    15361 tcgctgacgg tgacggtttg cggctttccg gcggaaggtt gccacagacg ttgatactgg
    15421 ttaagacggt tgagcagtcg catataaacc cggaacaata ttatttaact attcactatt
    15481 acttccgtat atatcaggtg atactcaatc accattaact gtgtcacaga gtggagaaag
    15541 aaatggctcg tctggcagca tttgatatgg atggcacttt attgatgcct gaccatcatt
    15601 taggtgagaa aaccctctct actttggcgc ggctgcgtga acgcgacatt accctcactt
    15661 ttgccacggg gcgtcatgcg ctggagatgc agcatattct cggggcgcta tcgctggatg
    15721 catatttgat tactggcaac ggaacgcgcg tgcattctct ggaaggtgaa cttttacatc
    15781 gtgatgattt acctgcggat gtcgcggagc tggtgctgta tcagcaatgg gatacccgag
    15841 caagcatgca tatcttcaat gacgatggtt ggtttaccgg gaaagagatc cctgcgttgt
    15901 tgcaggcatt tgtctatagc ggttttcgtt atcagataat cgatgtcaaa aaaatgccac
    15961 tcggcagcgt caccaagatc tgcttctgtg gcgatcacga cgatcttaca cgcttgcaga
    16021 tccagctata cgaagcatta ggcgagcgtg cacatttatg tttttccgcc acggattgcc
    16081 tcgaagtgct gccggtgggc tgcaataaag gcgctgcatt gacggtgctg acccaacatt
    16141 taggtttatc gttacgcgat tgtatggcct ttggtgatgc gatgaacgat cgcgaaatgt
    16201 taggcagcgt cggtagcgga tttattatgg gcaatgcgat gccgcaactg cgcgcggagc
    16261 tcccgcattt accggtgatt ggacattgcc gaaatcaggc tgtctctcac tatttgacgc
    16321 actggctgga ctatccacat ctaccttatt cccccgaata acgagatccc ttccagcacc
    16381 gggcaattgc ccggtttttt ttgcgttgaa tttgtcattt tgtgccgtgg tgcttaaacc
    16441 gcacagaata aattgtcgcg atttcacctt taaaatagaa ttaaaagaga aaaaaattct
    16501 ctgtggaagg gctatgttag ataaaattga ccgtaagctg ctggccttgc tacagcagga
    16561 ttgcaccctc tctttgcagg cactggctga agccgttaat ctgacaacca ccccctgctg
    16621 gaagcgtctg aaacggctgg aggacgacgg tattcttatc ggcaaagtcg ccctgctgga
    16681 tccggaaaaa ataggcctcg gcctgaccgc ctttgtgctg ataaaaacgc aacatcacag
    16741 cagcgaatgg tattgccgct ttgtcacggt ggttaccgaa atgccagaag tgctggggtt
    16801 ctggcgcatg gctggtgaat acgattatct gatgcgcgtc caggttgccg acatgaaacg
    16861 ctacgacgag ttttataagc gtctggtaaa cagtgtaccg gggctgtcgg acgtcacttc
    16921 cagcttcgcg atggaacaga ttaaatacac cacttcttta cccatcgaat aaatatccag
    16981 aatcaagtca ggacacaacg cgtgcgatta tttgctcaat taagctggta tttccgtcgg
    17041 gaatggcgtc gctatctcgg ggctgtcgcc ttgcttgtca ttatcgcgat gctgcaactg
    17101 gttccgccaa aagtggttgg tattgttgtt gatggcgtga cagaacaaca ctttactacc
    17161 gggcagatcc tgatgtggat cgccaccatg gtgctgattg ccgttgtggt ttatctcctg
    17221 cgttacgtct ggcgcgtatt gctgtttggt gcgtcttatc aactggctgt tgaactgcgt
    17281 gaagattatt accgccagct aagccggcag catcctgagt tttacctgcg tcatcgcacg
    17341 ggcgacctta tggcccgtgc gacaaatgac gtcgatcgcg tcgtgtttgc cgccggagaa
    17401 ggggtgctga cgctggtgga ttcactggta atgggctgcg ctgtgttgat tatgatgtct
    17461 acgcaaatta gctggcagtt gaccttattt gccctgttgc cgatgccagt gatggcgatc
    17521 atgatcaagc gcaacggcga tgccttgcat gaacgcttta agctggcaca ggcggcgttt
    17581 tccagtctta atgaccgcac ccaggaaagc ctcaccagta tccgcatgat caaagccttt
    17641 ggtctggaag atcgtcagtc ggcgttattt gccgcggatg ccgaagatac cggcaaaaaa
    17701 aacatgcggg tggcgcgtat tgatgctcgt ttcgacccga ccatctatat cgcgattggt
    17761 atggcgaact tgctggcgat cggcggcggt agttggatgg tggtgcaggg cagtttaacg
    17821 ctgggccagc tcaccagttt tatgatgtat ttaggtctga tgatttggcc aatgctggcg
    17881 ctggcatgga tgtttaacat tgtggaacgt ggcagcgcgg cgtatagccg aattcgtacc
    17941 atgctggccg aagcgccagt cgttattgat ggtagtgata aggtgccaga aggtcgtggt
    18001 gaactggatg tgaatattcg ccagtttacg tatccgcaga ctgaccatcc tgcgctggaa
    18061 aacgtcaatt tcgccctgaa acccggtcag atgctgggta tctgcgggcc gactggttcc
    18121 ggcaaaagta ccctgttgtc gctcattcag cgtcatttcg acgtcagcga gggtgatatc
    18181 cgttttcatg atattcctct gacgaagtta caactcgata gctggcgtag ccgtctggcg
    18241 gtggtcagcc agacaccatt ccttttttcc gacaccgtgg cgaacaacat cgcgttgggt
    18301 tgcccgaatg ccacccagca agagattgaa cacgtagcgc ggttagccag cgtacatgac
    18361 gatattttgc gtctgccgca aggttacgac acagaagtgg gcgagcgcgg tgtgatgctt
    18421 tctggcggac aaaaacagcg tatctccatt gctcgagcgt tattagtcaa cgcggaaatc
    18481 ctcatccttg atgatgcgct ttcagcggtg gacggacgca cagagcacca gatcctgcat
    18541 aacctgcgtc agtgggggca gggaagaacg gtaatcatca gtgcccatcg cctttctgca
    18601 ctgacggaag ccagtgaaat tattgtgatg cagcacgggc atatcgccca gcgtggcaat
    18661 catgatgtgc tggtacaaca aagcggctgg tatcgcgata tgtatcgcta tcaacaactg
    18721 gaggcggcgc tcgacgacgc cccggaaatt cgcgaggagg ccgtcgatgc gtagttttag
    18781 ccaactgtgg ccgactctca agcgtctgtt agcgtacggt tcaccgtggc gtaaaccgtt
    18841 ggggattgcg gtcctgatga tgtgggttgc ggcggcggca gaagtcagtg ggccgctgct
    18901 tatcagctat tttatcgaca atatggtagc gaaaaataac ctgccgttga aagtggttgc
    18961 agggctggcg gcggcgtatg ttgggctgca actgtttgca gccgggctac attacgcgca
    19021 gtcgctgctg tttaatcggg cggcagtagg cgtagtgcaa cagttgcgta ccgacgtgat
    19081 ggatgctgcg ttacgccagc cattaagcga gtttgatacc caacccgtcg ggcaggtgat
    19141 ttcccgcgtc actaatgaca ctgaagtgat ccgcgatctc tacgttaccg tagtggcaac
    19201 tgtcctgcgt agcgccgcac tggtgggcgc gatgctggtg gcgatgttca gcctcgactg
    19261 gcgaatggcg ctggtggcga taatgatttt cccggtggtg ctggtggtaa tggtgatata
    19321 tcagcgttac agcacgccga ttgtccgtcg tgtgcgcgct tatctggcgg atatcaacga
    19381 cggctttaac gaaatcatca atggcatgag cgttatccag cagttccgtc agcaggcgcg
    19441 atttggcgaa cgtatgggtg aggccagtcg ttcacactat atggcgagga tgcaaaccct
    19501 gcgcctcgac ggttttctgc tgcgtccgct gctgagtctg ttttcatcgc tcattctttg
    19561 tggcttgttg atgctgtttg gcttctccgc cagcggcacc attgaagtgg gcgtgttgta
    19621 tgcgttcatc agttatttgg ggcgacttaa cgaaccctta atcgaactga ccacgcagca
    19681 ggcgatgctg caacaggctg ttgttgctgg tgagcgcgtt tttgagctga tggacggacc
    19741 gcgccagcaa tatggcaatg atgatcgctc gttgcagagc ggcaccatcg acgtcgataa
    19801 cgtgtcattt gcttatcgcg atgacaatct ggtgctaaag aacattaatc tctctgtgcc
    19861 ttcgcgcaac tttgtggcgc tggtcgggca taccggcagt ggcaaaagca ccctcgccag
    19921 tttattgatg ggctattacc cgctaacgga aggtgagatt cgccttgatg gtcgtccatt
    19981 aagtacgcta agtcacagcg cgctgcgcca gggcgtggca atggtgcagc aagatccggt
    20041 ggtgctggcg gataccttcc tcgccaacgt gacgctggga cgtgatatct ctgaagaacg
    20101 cgtctggcag gcgctggaaa ccgtgcagct ggcggagctg gcgcgtagca tgagtgatgg
    20161 tatttacacg ctgcttggcg agcaggggaa taatctctca gtcgggcaaa agcaactgct
    20221 ggcactggcg cgcgtgctgg tggagacgcc gcaaatcctg atccttgatg aggcaaccgc
    20281 cagcattgac tccgggactg aacaggcgat tcaacatgct ctggcggcgg tgcgtgaaca
    20341 tactacgctt gtggtgattg ctcaccgctt atcgactatt gttgatgccg acaccattct
    20401 ggtgcttcat cgtgggcaag ccgtggagca gggcactcac cagcaactgc tggcggccca
    20461 gggacgatac tggcagatgt atcaactgca acttgcgggc gaagagctgg cagccagcgt
    20521 gcgtgaagag gaatcattga gcgcctgaat agcgcaatat ttcatcgttg gtgcaaaaat
    20581 gtaacgcact gtgcactgtc atagtgcgtt ttcattttca atcttcttaa cttcctgctc
    20641 tctttctcgt ttttcatttc tggcacaccg cttgcaatac cttcttcgtg tagcagaacc
    20701 attaccgaat tctgaccgga ggggatctat gaagctggtg accgtgataa tcaaaccatt
    20761 caagctggaa gacgttcgtg aagcgttatc ttccattggt attcagggcc tgaccgtcac
    20821 cgaagtgaaa ggttttgggc gtcagaaagg gcatgccgag ctgtaccggg gggcggaata
    20881 cagcgtcaac ttcctgccaa aagtaaaaat tgatgtggcg attgctgatg accaactcga
    20941 tgaagtgatc gatatcgtca gcaaagcggc ttacaccgga aaaattggcg acggcaaaat
    21001 cttcgtcgct gaattgcaac gcgtcattcg tattcgtacc ggcgaagccg acgaagcggc
    21061 gctgtaatct ctggcacaca gcaacaggaa cgaaaaatga agatagcaac gataaaaact
    21121 gggcttactt cactggcgat gctgccggga ctggtaatgg ctgcacctgc ggtggccgat
    21181 aaagccgaca atgcatttat gatgatttgt actgcgctgg tgctgtttat gactattccg
    21241 gggattgccc tgttttatgg agggctgatt cgtggcaaaa acgtgctgtc gatgctgacg
    21301 caggtgaccg tgacgtttgc actggtctgt attctctggg tggtttacgg ctactcgctg
    21361 gcgtttggcg aaggcaacaa cttcttcggc aacattaacg ggttgatgct gaaaaacatt
    21421 gaactgacgg cggtgatggg cagcatttat cagtatatcc acgtggcgtt tcagggatcg
    21481 tttgcctgca ttaccgtagg cttgatagtt ggggcgctgg cggaacgaat ccgcttctca
    21541 gctgtgttga tttttgtggt ggtatggctg acgctctctt acattccgat tgcgcatatg
    21601 gtctggggcg gcggtttgct ggcttctcac ggtgcgctgg atttcgcggg tggcacggta
    21661 gtgcacatta acgccgcaat cgccggtctg gtgggcgcgt atctgatagg caaacgcgtg
    21721 ggcttcggca aagaggcgtt taaaccgcac aacctgccga tggtcttcac cgggactgcc
    21781 attctctata tcggttggtt tggctttaac gccgggtcag cgggcacggc gaatgaaatc
    21841 gcggcactgg catttgtgaa tactgtggtc gcaaccgcgg cggcaattct tggctggatc
    21901 ttcggtgaat gggcgctgcg tggtaagcct tcactgctgg gagcgtgttc tggcgcgatt
    21961 gccggtctgg tcggcgtgac gccagcctgt ggttacattg gggttggcgg cgcgttgatt
    22021 atcggcgtgg tagctggtct ggcgggcttg tggggcgtta ccatgctcaa acgcttgctg
    22081 cgggtggatg atccctgcga tgtcttcggt gtgcacggcg tttgtggtat tgtcggctgt
    22141 atcatgaccg ggatttttgc cgccagctcg ctgggcggcg tgggcttcgc tgaaggtgtg
    22201 acgatgggcc atcagttgct ggtacagctg gaaagcatcg ccattacgat tgtctggtct
    22261 ggtgtggtgg catttatcgg ctacaaactg gcggatctga cggttggtct gcgtgtaccg
    22321 gaagagcagg agcgagaagg gctggacgtc aacagccacg gcgagaatgc ctataacgcg
    22381 taacaagcac tgcaaaaaac agccggacgg ttttcacctc cggctatttt tttaattgtg
    22441 attacgcatc accccttcct gaacggtcga ggcaaccagc acgccgtcct gggtataaaa
    22501 ctcaccgcgc acaaagccgc gtgcgctgga cgccgaggtg ctttccacgc tatacagcag
    22561 ccattcattc aaattaaacg ggcgatggaa ccacatggaa tggtcaatgg tggcaatctg
    22621 aatccccggt tcgagaaaac cgatgccgtg cggctgtaga gctaccggca ggaaattaag
    22681 atcagaagca taaccgagca gatactgatg aacgcgcaga tcatccggca cgctaccatt
    22741 tgcgcggatc cacacctgac gatgcggttc tgcgacgtga cctttcagtg ggttatgaaa
    22801 ctccaccgga cggacttcca gcggacgatc gcagatgaat ttatctttca gcactggcgg
    22861 cagcaggtgc gccagcgatt gggcgatttg cgtttctgaa ggcaggccat caggcgctgg
    22921 tgcggacggc attgtttttt gatgttcgaa acccgcttct ggtgcctgga aagaggcagt
    22981 catataaaaa atcggtttgc cgttttgaat agcagcaacc cggcgggcgc tgaagctgtt
    23041 accgtcacgc agtgtttcga catcataaat aatcggcttc ttactatcgc cagggcgaag
    23101 aaagtagctg tgaaaagaat gtaccagccg ctcgtcaggg acggtctctt ttgcagcata
    23161 caaggcctga cccacgacct ggccgccaaa tacctggcgt aaacctaaat cttcactctg
    23221 gccgcgaaag agtccttcct caattttttc cagatttaac aatgtcagta aattttttag
    23281 cgcctgactc atataactct ccagtaacaa agctgccgca gcaagccaaa gtgagttgag
    23341 tataacgcaa atttgccact ggtccgatgg gtgcaatggt ctgaattacg ggctaattac
    23401 aggcagaaat gcgtgatgtg tgccacactt gttgacgtta ctattttgtt aaccactctt
    23461 ccggcgagga aagttagccc gctggtgcat tgataataag gagaagtgaa tgaaactcgt
    23521 gcacatggcc agtggtttag cggttgcgat tgcgttggcg gcttgcgcag ataaaagcgc
    23581 ggatattcag acgccagcac cggctgcaaa tacgtctatt tcagcaacac aacaacctgc
    23641 tatccagcaa ccgaatgtct ccggtaccgt ttggatccgt cagaaagtcg cactgccgcc
    23701 tgatgccgtg ctgaccgtga cactttctga cgcgtcgtta gccgatgcgc cgtcgaaagt
    23761 gctggcgcag aaagcggtgc gtaccgaagg taaacagtca ccattcagct ttgttctgcc
    23821 atttaacccg gcagatgttc agccgaacgc gcgtattctg ttgagtgcgg cgattaccgt
    23881 gaatgacaaa ctggtattta ttaccgatac cgttcagccg gtgatcaacc agggcggaac
    23941 taaagccgac ctgacattgg tgccggtgca gcaaaccgcc gtgccggttc aggctagcgg
    24001 tggcgcaacg actaccgtac cttcaacttc accaacccag gtgaatccgt cttcggcagt
    24061 tccagctcca acgcaatatt aagcgaagtt aaccctctcc gcccggagag ggttagtagt
    24121 tccagcgata aagctgcaag tcgatttgcc cgcttcccga caccatcaca ccttctgcca
    24181 gtaatgcctg tcgctgacgc tgtaaatccg gtccggtcag tgaaatggtg ccgtggcgat
    24241 taaccacccg gtgccagggc aaggtgctgc cttcggggag acgctttaac acaccgccaa
    24301 cctggcgcgc ggcacggggc gagcccgcca gtttcgccac atcaccgtaa gtggtgacat
    24361 agccttcggg aatagcggct acgatttgcc agacgcgttg gggaaatgaa tcttcttttt
    24421 ccatcttttc ttcctgaggt aatttttcag cataatctgg aaaaacgccc gagtgaagcc
    24481 gcattgcgca agaaaccagc atctggcacg caatgggttg caattagccg gggcagcagt
    24541 gataatgcgc ctgcgcgttg gttctcaacg ctctcaatgg gggctctgtt ggttctcccg
    24601 caacgctact ctgtttacca ggtcaggtcc ggaaggaagc agccaaggca gatgacgcgt
    24661 gtgccgggat gtagctggca gggcccccac ccatttctgc ctcctaccat ttcgtcaaaa
    24721 aatcccaaca tggctaaact ttaaccacaa ctgacgtcgc aagaattgtc tggctgcgca
    24781 gtacgcttcg gaggtatgtc tgatgaagta tgttgatggt tttgttgttg ccgttcctgc
    24841 cgataaaaag gatgcctatc gggaaatggc cgctaaggcc gcgccattat ttaaagagtt
    24901 cggggcgctt cgtattgtcg aatgctgggc cagcgatata ccggatggta aagtgaccga
    24961 ttttcgtatg gcggtgaaag cggaagagaa tgaagaggtt gtctttagct ggattgaata
    25021 cccttcaaaa gaggtccgcg acgctgctaa tcaaaagatg atgtcggacc cacggatgaa
    25081 agagttcggc gagtccatgc cgtttgatgg taagcgaatg atctatggcg gattcgagtc
    25141 aatcatcgac gagtaggtag cgtgacgggc tgtgcggcgt gccgtcagcc ctataattca
    25201 caaatgttgc tcggcccagc gtaagaaatc ctctttcggt aatgccttgc tgtagagcca
    25261 gccctgaccg tagtgcacgc catgctggcg tagccactct tcttgtttac tggtttcgat
    25321 tccctccgca accattttca gtttcagtgt ttttgccatt tcgatgatat gtggcgtgac
    25381 attcttatat tccagcgcat caacgaaaga tttatcgatc ttcagaatgt cgacatccag
    25441 atcctgtaaa tagcttaaac ttgaataccc tgtaccaaaa tcatcgagat aaatttcatg
    25501 gcccgcctcc cggtagcgag aaattatcgg ggcgctggtt ttcggatcgg caaactcgcg
    25561 ttcagtgagt tcaagcgcga tctgtctggg attaacctga tagtgattga tcatttcacg
    25621 cagcaattgc gggatttttt ccgaggtgag cacggtggat tcaagattga tcgaaatatg
    25681 ctgctgagga tgctgacgca gccagtcgcc catatcttca aagacgcttc ttataatcag
    25741 tagcgtcaat ggctcagaaa ggcccgtttg ctgtgccagc ggaataaaac tatctggtga
    25801 caaccaacta ccgtctgtct gcggccagcg cgccagtgcc tcagcaccga caattttgcc
    25861 attggctaag gagacaatcg gctgatagtg cacgcaaata tcacgatttt cgatagcatc
    25921 ctgcagccga tgatgcggtg actggatacg gcgcaaaata cgcagcacaa acatcgctgc
    25981 cagcaggcca atcaccaacc cgagcggtaa ccaaataaag acttgccgat accagccttt
    26041 ctgcaacatt ttcgttgaag cccaggtgat gatcgaaata ttcatctccg gtaagggcag
    26101 gatatcgtaa atgattccat tattttcgat atgctcaccg ggtgtttttt gtagcctggt
    26161 aataattccc tgagcaagtt catcgctgct ggttatgaca acgttatggg cattgccaat
    26221 aatggcggca tcaatttgcc atgagctata gggaatgaca tcaataaagg aagcggggtc
    26281 gatcatgacg acataatgtg ccgttcccat ggcgaccatg taacggataa tgcctaaatc
    26341 gttatgcgat gttaaccaga cacgataacc atctttcgaa attttacctg gctcggggaa
    26401 ggtatcgggc gggctttcat gctccagaga cgaacattgg ggaacgttgt tatcgatata
    26461 aaccacttcc tgaatatagc gataactgta agatacccga cgcatttcca tgagatgggc
    26521 ttcgctacag gcagcgcctt gccatctttc cagctcttgc agcgcatctt tcccttgtgt
    26581 cgccacctta ttggcccgaa tagcgacgcg ggaggaatac gtattcagct cttcaataaa
    26641 tgacgtttct acctgctgat gggccagcca gatgcttaag ccgacaggca gcaatactga
    26701 aagaatcagt actcccgaaa taaggccgac cagatgtcgt gttctcacca gcatatcctt
    26761 attaacacag tgtagggaaa gtatatctga ttgtggaaaa aggctggcag gagataagga
    26821 ggtagagctg gtatttcatg aagtgatggg tggaagcaat cgttgtcatt tgaccaggcg
    26881 tagagcttta cgcctggtat gtacccctaa cttaagatgt tgattcggct cgagcgaggg
    26941 taaaaatatc gtaaaaagat agttcacctt tacggacgat cattttttgc agttgttctt
    27001 tatgaacatc cgcgacacgt ggagagttca ggatggcttc aaccagcgcg gcaggtacaa
    27061 agcgcgtgtt gtaccattcg ccgttatagc aaacgcggaa atccagcacg tcgatatctt
    27121 catagcggta gcgtgggtag acatcgaaaa agaagaaacc attaagcagg ataaataata
    27181 caaccagtag ccagacagag cctgaaagcg tttcggattg caacatcacc gccagggtag
    27241 caaaaaaagc aacgtacata ccgataaaca gccccggatg tttacggata aaactgatac
    27301 taaagcgggg tttgttgtcg cgtttttcgc gggtattcag agactcaatc gtttcggtca
    27361 gcaggcgttg tatttcggtc atcgtttgcc ttcgtgatat ctgcaacttt ccagggaaca
    27421 ccctatttta acgtgggaat agtagagggg aagtaacaga tttgccacga ttttgcataa
    27481 cagttgagaa aagctggtta caatttttta atgattctgg ctggattacc gcccacgaca
    27541 acgttgtccg ggacatcttt tgtgacaact gcacctgagg ctaccacgac gttatcacca
    27601 atggtcacac cagggttaat gactgcacgt ccgccaatcc agacgttatt accgatggtg
    27661 acgggtttcc ccagttcagc accgctatta cgtgctacag ggtcgatggg atgtgttgcc
    27721 gtgtagatat gaacgcctgg tgccaacata cagttatcac caatgcgaat agggcagaca
    27781 tcaagcatca cgcaatcgaa gttggcgaaa aaattattgc cgagaaaaat gttatagcca
    27841 tagtcacagc gaaacgttgg ctcaatataa gcctctgtca cctgaccgaa taaatcagca
    27901 agaatttgct ggcgtaatgt gtgctcttcc gccagggaat gattgtatcg gtgaataagc
    27961 cgacgagcgc gcaggcgatc gcgagataac gcctcatctg ccgagtgata caactcacca
    28021 gcaatcatct tttctttttc tgtgctcatt actgacttcc ggttgctgaa aatgcggcaa
    28081 cacagtaaga cgaaaagtca gtgaagagaa cgttcccatt acatctgtgt gatgaagatc
    28141 acatagggta tgccttcatg gcgaaaaaaa gtataaaatt cttaataaac agccggttat
    28201 agctccgaaa gcgagttatt tattagcgaa taaatttcca tactgaggaa gggatcttgt
    28261 cgtacagttt attcatggtc aattcggcga ggcggtgatc tgcggctgag taaaataccg
    28321 ccagttcatt atctgataat tcgtatttat ttttctcgat aacacgctcc agcgtgtcaa
    28381 ttgtctggca acgacgtaaa cgcattaaat aatcggtttt cgttaaaggt ttttcggaca
    28441 tacttctacc catggttgta ataattctaa caagataaac tcgcaggatt ctctttcgtc
    28501 gcattgacaa aacaacggaa tagtcgatta cctgacttcc gccatttctg aagatcctgc
    28561 atattaatac cataactact gaacaacata aaggtgtcat ccagatattc gtcgatctgc
    28621 tcaatgagct tattgtcttc attatactta attttgtaat taagtgcgaa ggtcgcaata
    28681 tgctcaatca gttcattcaa ctggaggttg atcgccgagg ttgggtcgtt aacccagcca
    28741 tgattgcttt cttcaaggtt tgcaaggcag tcatgataca gggtttcaca gagaaactta
    28801 agctgtgcga tatcatgtct tttgggtgag tattcatcca taacgcgtcc ccttcttagc
    28861 ggttgaacta acggacacct ttcgggatgg aaaaaactta ctgacctgga cttgccctct
    28921 cgtttgttag cttaactata agccactctt tgcaggtttt catcgcatta ttacgaaaaa
    28981 ttacaattag gaaggaagcg ggacaggact cactttttca tactagcacc aacccgcagc
    29041 aggtaaaagc agtttgctcg aactgttcat aacatttcag aattatcgcc agaagaaatt
    29101 ggtgacacta ataccaggat tgctctgaat atgacgtaat aaccgaggaa tgaataaaga
    29161 a
//
LOCUS       DACADF010000051        28940 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.051, whole genome shotgun sequence.
ACCESSION   DACADF010000051 DACADF010000000
VERSION     DACADF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.051"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            90..332
                     /locus_tag="ISB30_002109"
     CDS             90..332
                     /locus_tag="ISB30_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989739.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            447..1085
                     /locus_tag="ISB30_002110"
     CDS             447..1085
                     /locus_tag="ISB30_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAP1989740.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1082..1819
                     /locus_tag="ISB30_002111"
     CDS             1082..1819
                     /locus_tag="ISB30_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAP1989741.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSDAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALMTRMAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQS
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1819..3915
                     /locus_tag="ISB30_002112"
     CDS             1819..3915
                     /locus_tag="ISB30_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAP1989742.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDPREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSIGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3962..4240)
                     /locus_tag="ISB30_002113"
     CDS             complement(3962..4240)
                     /locus_tag="ISB30_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989743.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4455..4865
                     /gene="psiE"
                     /locus_tag="ISB30_002114"
     CDS             4455..4865
                     /gene="psiE"
                     /locus_tag="ISB30_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAP1989744.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4959..5849)
                     /gene="malG"
                     /locus_tag="ISB30_002115"
     CDS             complement(4959..5849)
                     /gene="malG"
                     /locus_tag="ISB30_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAP1989745.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5864..7408)
                     /gene="malF"
                     /locus_tag="ISB30_002116"
     CDS             complement(5864..7408)
                     /gene="malF"
                     /locus_tag="ISB30_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAP1989746.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNDQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7562..8752)
                     /gene="malE"
                     /locus_tag="ISB30_002117"
     CDS             complement(7562..8752)
                     /gene="malE"
                     /locus_tag="ISB30_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAP1989747.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFETDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            9117..10232
                     /gene="malK"
                     /locus_tag="ISB30_002118"
     CDS             9117..10232
                     /gene="malK"
                     /locus_tag="ISB30_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAP1989748.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10304..11644
                     /gene="lamB"
                     /locus_tag="ISB30_002119"
     CDS             10304..11644
                     /gene="lamB"
                     /locus_tag="ISB30_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAP1989749.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            11877..12797
                     /gene="malM"
                     /locus_tag="ISB30_002120"
     CDS             11877..12797
                     /gene="malM"
                     /locus_tag="ISB30_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAP1989750.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVSGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSVPDIPDPIARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            12976..13473
                     /gene="ubiC"
                     /locus_tag="ISB30_002121"
     CDS             12976..13473
                     /gene="ubiC"
                     /locus_tag="ISB30_002121"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAP1989751.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            13486..14358
                     /gene="ubiA"
                     /locus_tag="ISB30_002122"
     CDS             13486..14358
                     /gene="ubiA"
                     /locus_tag="ISB30_002122"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAP1989752.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARTLFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14513..16936)
                     /gene="plsB"
                     /locus_tag="ISB30_002123"
     CDS             complement(14513..16936)
                     /gene="plsB"
                     /locus_tag="ISB30_002123"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAP1989753.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTKQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            17107..17475
                     /locus_tag="ISB30_002124"
     CDS             17107..17475
                     /locus_tag="ISB30_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP1989754.1"
                     /translation="MANNTTGFIRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17585..18193
                     /gene="lexA"
                     /locus_tag="ISB30_002125"
     CDS             17585..18193
                     /gene="lexA"
                     /locus_tag="ISB30_002125"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAP1989755.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            18212..19591
                     /gene="dinF"
                     /locus_tag="ISB30_002126"
     CDS             18212..19591
                     /gene="dinF"
                     /locus_tag="ISB30_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAP1989756.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNDTWFVAT"
     gene            19707..19916
                     /locus_tag="ISB30_002127"
     CDS             19707..19916
                     /locus_tag="ISB30_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP1989757.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(19958..20473)
                     /gene="zur"
                     /locus_tag="ISB30_002128"
     CDS             complement(19958..20473)
                     /gene="zur"
                     /locus_tag="ISB30_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAP1989758.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            20629..21624
                     /gene="dusA"
                     /locus_tag="ISB30_002129"
     CDS             20629..21624
                     /gene="dusA"
                     /locus_tag="ISB30_002129"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAP1989759.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            21758..22000
                     /gene="pspG"
                     /locus_tag="ISB30_002130"
     CDS             21758..22000
                     /gene="pspG"
                     /locus_tag="ISB30_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAP1989760.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQHYDRWRY"
     gene            complement(22165..23148)
                     /locus_tag="ISB30_002131"
     CDS             complement(22165..23148)
                     /locus_tag="ISB30_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAP1989761.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVKNKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSMHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDARRAHEILESRATQGSSLLIP"
     gene            complement(23254..25290)
                     /locus_tag="ISB30_002132"
     CDS             complement(23254..25290)
                     /locus_tag="ISB30_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAP1989762.1"
                     /translation="MIDETLTALTDIKMPHYAPLAQASGSIDLNGTILPEYCCNTLVL
                     GSGAAGWRAAVELKRQNVDVMVASSKAFWGTSACSGSDKQTLHTANTRANGDHFLTLS
                     NTLAAGGAMDHDTAYVEAVGSVNTCEVLKYLGLDLPEDLFGATLRYQTDHDEFGRATS
                     CGPRTSRLMVKVLAQEAMRLNIPLCDHTTAIHILTSGEGENRRVVGVIAIDKACRDNP
                     WRMVVIRCQHLVLATGGPGELYRDSVYPVNCFSALGMALEAGITLVNLTESQFGIGTP
                     RSQFPWNLSGTYMQAMPRIYSQDHSGRQYNFLAAYYPTTRMLASAIFRKGYQWPFHAE
                     RMLEYGSSLLDVAVYEETQKGRDVFLDFLREPEPAADGTSFNLQELDDDVIDYLQQNH
                     ALLAQPLARLTQMNPLAIRLYQMHGHDLTASPLKFTLNNQHLNGGIEVDIWGRTSLTG
                     CYAAGENAGTHGVTRPGGAALNAGQVFARRCALHIAHQKSQDRPLERQQILSTINEAQ
                     QYLNQGLLRDDVRETIQNTMSHHAAILCHTQGINTAAQTLATLCEDIRREGIQCDENS
                     LAQSFQWRQSAQLALAVLRSLQCYVENGGGSRGARAIYDGEAGIAPQTPGGPLLAWRF
                     RPENQDARQYMICVCREANDYQTWTRPCRERGVLTLTNFERQWQNWIAATPFQY"
     gene            complement(25301..26581)
                     /locus_tag="ISB30_002133"
     CDS             complement(25301..26581)
                     /locus_tag="ISB30_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP1989763.1"
                     /translation="MNMTTSVPQTPSLFRRIMTKLAAFAPGIFLIGYSIGTGSITTMA
                     KAGANYGVSLMWAVALSCIVTWFLIRIYGRYASVTGERALTAFRRHIHPGVAMLFLIG
                     LTVAVSGSIMGVMGILADVMATWSTTWFSGGISALAWAIAMVIMINVMLWTNSNAGFD
                     KILSALVGIMGLAFVINFFIMMPPVADLLAGFIPSVPDPVAGDNSTPFLIISGMVGTT
                     VAPAMFLMRTTLVKSNGWGVDNLPHQNRDAIVSAGMMFIISAAVMAAAAGTLYQTGTG
                     LNNASQMVIILEPIAGSAAVGLFVIGIVAAGLSSQFPNLLLTPWLLADWRDTSNEVSP
                     GYRILMTVMSLLCLVVPLFHARPVLVMIASQAFNAIILPLTVLCMFWLANKPSLMGKY
                     RCSRWENVMFIAISVFTLIMGYMAMSGLIESLMK"
     gene            complement(26608..27387)
                     /locus_tag="ISB30_002134"
     CDS             complement(26608..27387)
                     /locus_tag="ISB30_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAP1989764.1"
                     /translation="MNNGTIDLQRNGFIATLVINRPAKLNALTPEMLEQLLAHCHTLE
                     TDSEIRVVLLTSASEKAFCVGADIHRWTSLSALDMWRNWIRRGHQAFDALAQLPQPVI
                     ALLHGLAYGGGLELAATADIRICSAEAQMALPETGLATVPGWSGTQRLTALLGRSLVK
                     ELVFTGEPLDAQRALSCGLVNRVVAREALHDAGLQLAQRIAQRAPVAVQIAKQVIDAG
                     EGIAVASTLEALAGAFSATTEDAHEGSQAFTEKRQPHFQAR"
     gene            complement(27380..>28940)
                     /locus_tag="ISB30_002135"
     CDS             complement(27380..>28940)
                     /locus_tag="ISB30_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HAP1989765.1"
                     /translation="RTITAPEAADLIVDGAVVTVSSSSGMGCPDAVLAAIGERFQRAG
                     HPQNITSIHPIAAGDMYGVKGIDHLAQPGLLAKVIAGSYPSGPSSMTPPLIWQMINDN
                     AIPAWNLPSGILFDMHREAAAHRPGVLTQTGMDTYVDPLLQGGAMNEKAAQQSLVERV
                     RFANDDWLFFPSIVPQVAIIRATTADERGNLTYEHEGAYLGPLEQATAVRNNGGIIIA
                     QVKRQVAAGSLKPKEVRIPGVLVDYIVIAPEQTQTTQTQYEPAISGEISRPLSAFRYM
                     EHGPARVIAQRVAQELQSGDAVNIGFGISANVPRILLEQGRHGDVTWLLEQGAIGGVP
                     LLEFQFGCASNAEAFLPSPQQFTYFQGGGFDLTLMSFLQIGADGSVNVSHLPARPHVT
                     AGCGGFIDITSHAKRIIFSGFFNAGAQLQLEEGQLRIIKEGKAKKLVQDVAHVTFSGK
                     RAIRLGQQVQYITERCVLELTPDGLLVTEIAPGIDPERDILAQSDAPLRLADDLKLMN
                     PLYFQQGSHHE"
BASE COUNT         6842 a         7413 c         7752 g         6933 t
ORIGIN      
        1 ccgtaattcg catgctttag ttgtgtatac tcgatcccgc ccgaaatgtt ttgggtaaat
       61 ctccattcat tcaatgaagg gaaattgtta tgaaaaaagt tctgtatggc atttttgcca
      121 tatctgcgct tgcggcgact tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag
      181 gatcggcagc aacgtcggtt tcggcgggta gttcctccgc gaccagcgtc agcaccgtaa
      241 gctcggcggt gggtgtcgcg ctcgcggcaa ccggtggcgg tgatggttct aataccggga
      301 ccaccacaac cacgaccacc agtacccagt aataaagtat gtatccccaa aataattcga
      361 gtcatcgcat ctgaagcttg aagtatgaag gggattaacc ataaccacac tccggtgtgg
      421 ttattctgcc cctctggaga agagtcgtga agcgaccagc actcattctt atctgcctgc
      481 tattacaggc ctgttcagcc acgactaaag agctgggcaa ttcactgtgg gacagtctgt
      541 tcggtacgcc aggcgtacag ctgacggacg atgatattca aaatatgccc tacgccagcc
      601 agtacatgca gcttaatggc gggccgcagt tatttgtggt gctggccttc gctgaagacg
      661 gacaacaaaa atgggtcact caggatcagg ccacccttgt cacacaacat ggtcgtctgg
      721 tgaagacttt gcttggcggc gacaacctga ttgaagtgaa taaccttgcc gccgacccgc
      781 tgattaaacc cgcacaaatt gttgatggtg caacctggac gcgcacgatg ggctggaccg
      841 agtaccagca ggtacgctac gccaccgcac gctcagtctt caaatgggat ggcaccgata
      901 ccgtcaaagt cggcagcgat gaaaccccgg ttcgcgtgct ggacgaagaa gtctccaccg
      961 accaggcgcg ctggcataac cgctactgga tcgacagcga agggcaaatt cgccagtcgg
     1021 aacagtatct cggcgcggat tacttcccgg tgaaaaccac cctcatcaag gcggcaaaac
     1081 aatgattaaa caaactattg tcgcattgat tttgagtgtg ggggcgtcat cggtcttcgc
     1141 ggcgggaacc gtcaaggtat tcagcaatgg cagcagtgat gccaaaacgc tgacgggtgc
     1201 agagcattta atcgatctgg taggccaacc gcggctggca aacagctggt ggcctggtgc
     1261 ggtgattagc gaagagctgg ccacggcggc agcattgcgt cagcagcagg cgttgatgac
     1321 acggatggca gaactggccg cagactccag cgccgacgat gccgccgcaa ttaacgcctt
     1381 acgccagcaa attcaggcgt tgaaagtgac gggcaggcaa aaaatcaatc ttgatcctga
     1441 tatcgtccgc gttgccgaac gcggtaaccc gccgttgcag ggcaactaca cgctgtgggt
     1501 cggaccgccg ccgtccacgg tcacgttgtt cgggcttatc agtcgtcctg gcaagcagtc
     1561 attcactcca ggtcgcgacg tggcgagcta tctctctgac cagagcctgc tcagcggtgc
     1621 ggatcgcagc tacgcgtggg tggtttaccc ggacggacgc acgcaaaaag cgccggtggc
     1681 ttactggaac aagcgtcacg tagagccgat gcccggcagc attatttatg ttggcctcgc
     1741 ggactccgtc tggagtgaga cacctgatgc ccttaacgcc gacattcttc agactctgac
     1801 gcagcggata ccccaataat gaaaaaaaga catctgctta gcttactggc gctgggtatt
     1861 agcacagctt gctacggcga aacatatcct gcgcccattg gtccgtcgca atcggatttc
     1921 ggtggcgtag gattattaca aacgcccacc gcgcgcatgg cgcgggaagg ggagctgagc
     1981 ctgaactatc gcgataacga tcagtaccgt tattactcag cttcagtgca actcttcccg
     2041 tggctggaaa caacgctgcg ctacaccgac gtgcgcaccc ggcagtacag cagcgtcgaa
     2101 gcgttctctg gcgatcaaac gtataaagat aaagccttcg atctcaaact gcgtttgtgg
     2161 gaagagagtt attggctgcc gcaagtggcg gttggtgcgc gggatatcgg cggtacgggg
     2221 ctgtttgatg cggaatatct tgttgccagc aaagcctggg ggccgttcga ttttacactc
     2281 ggcctgggct ggggatatct gggcaccagc ggtaacgtga aaaatccgct ctgttcagcc
     2341 agtgataaat attgctatcg cgataacagc tacaaacagg cgggatctat cgacggcagc
     2401 cagatgttcc acggcccggc ctcactgttt ggcggcgtgg aataccagac gccctggcaa
     2461 ccgctgcgtc tgaaactgga gtatgaaggc aataattatc agcaggattt tgccggaaag
     2521 ctggagcaaa aaagtaagtt taacgtcggg gcgatttatc gcgttaccga ttgggccgac
     2581 gttaacctta gctatgaacg tggcaacact tttatgtttg gcgtcacgct gcgcaccaac
     2641 tttaacgatc tgcgcccgtc ttacaacgat aacgcccgcc cgcaatatca accgcagccg
     2701 caggatgcca ttttgcagca ttcggtggtg gcgaatcagt taacgctgtt gaaatacaat
     2761 gccggacttg ccgatccgca gatccaggcg aaaggcgata cgctgtatgt taccggcgag
     2821 caggtgaaat atcgtgatcc gcgcgaaggg atcatccgtg ctaatcggat cgtgatgaac
     2881 gatctgccgg atgggataaa aacgatccgc attacggaaa atcgccttaa tatgccacag
     2941 gtgacgacgg aaaccgatgt cgccagcctg aaaaatcatc tcgccggaga gccgttgggc
     3001 cacgaaacga agctggcgca aaaacgcgtc gagccagtgg ttccgaagtc caccgagcag
     3061 ggctggtata tcgacaaatc acgctttgat ttccatatcg atccggtgct gaaccagtcg
     3121 atcggtggcc cggaaaactt ttacatgtat cagctgggcg tgatgggaac ggcggatttg
     3181 tggctgacgg accatctgct gaccactggc agcctgtttg ctaaccttgc caacaactac
     3241 gacaagttta actacaccaa tccaccgcag gactcgcact taccgcgcgt gcgtacccat
     3301 gtgcgcgagt atgtgcagaa tgatgtctat gtgaataacc tgcaagccaa ctacttccag
     3361 catctgggca acggcttcta cggtcaggtc tacggaggtt atctcgaaac catgtttggc
     3421 ggcgtggggg cagaagtgtt gtatcgcccg ctggatagca actgggcgtt tggtctggat
     3481 gccaactacg ttaaacagcg cgactggcgt agcgcaaaag atatgatgaa attcaccgat
     3541 tacagcgtga aaaccggaca tctgaccgcg tactggacgc catctttcgc ccaggacgtg
     3601 ttagtgaaag cgagcgtcgg gcagtatctg gctggggata aaggcggcac gctggagatc
     3661 gctaaacgct ttgacagcgg cgtggtggtt ggcggctatg ccacgatcac gaatgtttcg
     3721 aaagaggagt acggcgaagg ggacttcacc aaaggcgtgt atgtctctgt accgctggat
     3781 ctcttctcgt ctggcccgac acgcagccgt gcggcgattg gctggacgcc gctgacgcgt
     3841 gacggtggtc agcaacttgg gcgtaagttc cagttgtatg acatgaccag cgaccgtagc
     3901 gtcaatttcc gctaagtcat gggaaaggtg ccaggtttcg cactcactgg cacctttatt
     3961 cttaatggcc tgtcgagcca tcaggaacgc agttcacccc cggtttgcga tctggtgtgt
     4021 aaaggcgaaa acattctgtc ttcctgtccg tcgatatcgg ttgcaggtct ggttgcctga
     4081 gcgactgggc catcattggg ctggagacat tgctttgcgg taggtcggct ttatcagcag
     4141 taccgagcgg accagcataa gcaggaagaa cagagactga taacatcaaa gcagcaaaat
     4201 aaggcttcat ttttaccacc tttatcaggt tacgtttcat ttgttccaga ggaacattgt
     4261 cgatttttcg cgcattgctg gtggctggga atcacctgaa tgggtgattt ttttgggctg
     4321 tgtgttttgc cagatgagac attgatggga gatgcccact taattgagct gaacaaaaca
     4381 tacacaaaaa atatagatct ccgtcacatt tttgcgttat acaggaagct cgccactgtg
     4441 aaggaggtac tgctatgacg tcactctctc gtccgcgcgt ggagtttatc tccaccattt
     4501 tgcagaccgt actcaatctt ggcctgttgt gcctcggcct gattttggtt gtcttcctcg
     4561 gcaaagaaac ggtgcatctg gctgatgtgc tgttcgcgcc agaacaaacc agtaaatatg
     4621 agctggtaga aggtctggtg gtttactttc tctatttcga atttatcgcg ctgattgtga
     4681 agtactttca gtccggtttt cacttcccgc tacgttactt tgtctatatt gggatcactg
     4741 ccattgtgcg cttgatcatc gtcgatcata aatcgccact ggatgtgttg atctactcgg
     4801 cagcgatcct gttactggtg atcaccctgt ggttgtgtaa ttcgaaacgg ctgaagcggg
     4861 agtaaaaaaa ggcgctccgt ggagcgccga ataacagtca caagttggga taacgtaagt
     4921 tgagggtgca gcggcataac attggcagaa caacatcttt aacctttcac accacctgcc
     4981 gtcaggccgt tgaccagcca gcgttgagcc agcaagaaga cgatggtgat cggtaatgca
     5041 gacatcacgg cagcggcggc aaagtcaccc cacaggtagt tttgcgggtt gaggtattgc
     5101 tgcatcccca cggccagggt gtagctgttt acgtcacgca gtaatagcga cgcgaccgga
     5161 acttcagtaa tggcagcgat aaacgacagg atgaacacaa ccgccagaat cggtactgac
     5221 agcggcaaca ggacaaggcg gaaggcctgc cacggtgtcg caccatccag cgccgccgct
     5281 tcttccagcg aactgtcgat ggtttcgaaa taacctttga tggtccagac atgcagcgca
     5341 atcccgccca gatacgcgaa aattacgccg ccgtgagtat tcaggccaat gaatggaatg
     5401 tactcaccca gacgatcaaa caacgcatac aacgcgacca gtgaaagtac tgccgggaac
     5461 atctggaaaa tcagcattcc tttcagcagc gtcgctttgc ctggaaagcg catacgggcg
     5521 aaagcgtagg cgcaggtggt ggagagcgcc acaatgccaa tcgcggaaat cccggcgacc
     5581 tttaccgagt tccacagcca cagcagtacc gggaatggcg gtggcgtaat gcgaccatca
     5641 gcctgttcca cgctaaaacc taacgccagt ttccagtgat cccaggagat ttgctccggg
     5701 atcagactac cggtcgcgaa gttcccctga cgcagcgaga tggcgacgac catcagcagc
     5761 gggaacataa ttgccgcgat aaaaagtagc agtagcaggt gagtaataaa caaacgtgct
     5821 ttttgcgatt tcggttggac cattgccatt tttgttatct cccttaatca aacttcattc
     5881 gcgtggcttt caggttcact atcgccagcg cgcccaccag caggaagatc agcgtggcaa
     5941 ttgctgctgc cagaccgaag tcctgacccc cgccgccttc aaaagcgata cggtaggtgt
     6001 agttaacaag caggtcggta taaccggctg gcgtggtcgt gccaagacga tccgggccgc
     6061 cgttggttaa cagttgaatc agcacgaagt tgttaaagtt aaaggcgaag ctggcgatca
     6121 tcagcggcgt cagcggttta atcagcagcg gcagcgtaat cttaaagaag ttctggaatg
     6181 gacctgcgcc atccattgct gaggcttcat acaaatcgtc cggaatcgct ttcagcaagc
     6241 ccatgcagag gatcatcatg tacggataac ccagccaggt gttgacgata atcagcatcg
     6301 tgcgggcggt ggtcggatcg ctgaaccagg cgggcttcac gccaaacagc gcgctcaaca
     6361 tcatgttgat ttcaccaaaa ctctggttaa acaacccttt gaaaatcaag attgaaatga
     6421 acgatggcac cgcgtagggc agaatcagca ggacgcgata gaccgctttg ccgcgcaacg
     6481 cttcccactg caccagacac gccagaacca tgccgaccgc caccgttaaa aagacagtga
     6541 tcagcgagaa caccacggtc cagacgaaaa tggcgaggaa cggtttctga atgccttcgt
     6601 cggtaaagac gcgggtaaag tttttccagc cggtggtcac ggtgtaaccg gggcttagct
     6661 tttcatcacc ccagttaccg tcggcagtga tggactggta aaaaccaatc tggtcgtttg
     6721 gacgatattt tacgccactc tgattattgg tcagcgtgcc gtcaccgtcg agtgtgtaga
     6781 gcggctgcgt gccagaaaac tggcgcaggg agctcatcat cactttgttg ccatccggca
     6841 gaatggcggt aatgtcactc agtgcctgac gattctgggt aattacgcgc agattcgcgc
     6901 gttcgccttc gggctgggcg gtcgtttctt tcagttgcag tttttgctcg ccgccaaatt
     6961 taaaagcgtc ggagaggtaa tttttgccgg tttcgccgtc gctgagcgcc agttgccact
     7021 catcgcccgc cggataaaga ccaaaattat agattttgcc tgcttgccag gagcgatcta
     7081 gcaacacttc ctgcgcacgt tcaaaagtca gctggttagt gctgctgtag ttggtgaagg
     7141 caatggcgat ggtgcagacc agagggaaga ggacgaataa tcccattcca gccattcccg
     7201 ggtaaacata gcgccaggcg taggctttac gattggcgaa aatatacagc cccgctgagc
     7261 tcaatatcag cgtggtaatg gcgaacaggt attccccttg tgcgtacatt aaaacaacaa
     7321 ggtaacccac cagcaggccg agcagaccta acactgacca tttcagcgcg tcgctttgcc
     7381 accaatgttt ctttttaatg acatccatgg ggttcttcct cattccagga cggataaggc
     7441 tttcatacct tatccgacaa caactgcctg atgcgacgct gacgcgtctt atcaggccta
     7501 catacgtttc aatttcgtag gccggataag gcgttcacgc cgcatccggc atttcacagc
     7561 attacttggt gatacgagtc tgtgcatctt tcagcgcagc atccacagtc tgacgaccgc
     7621 tggcggcgtt gatcaccgca gtacgcacgg cataccagaa agcggacatc tgcgggatgt
     7681 tcggcataat ttcacctttc tgagcgttat ccatggtggc ggcaatacgt ggatctttcg
     7741 caagttcatc ctgataggac ttcagcgcta ctgcacccag cggtttgtct ttattgaccg
     7801 cttccagacc ttcgtcagtc agcagatagt tttcgaggaa ctctttcgcc agctctttgt
     7861 tcggactggc ggcgttaata cctgcgctca gcacgccaac gaacggttta gatggttgac
     7921 ctttgaaggt cggcagtacc gttacaccat aattcacttt actggtgtcg atgttggacc
     7981 atgcccacgg gccgttgata gtcatcgctg tttcgccttt attaaaggca gcttctgcga
     8041 tggagtaatc ggtgtctgca ttcatgtgtt tgtttttaat cagatcaacc aggaaggtca
     8101 gacccgcttt cgcgccagcg ttatccacgc ctacgtcttt aatgtcgtac ttgccgtttt
     8161 cgtacttgaa cgcataaccc ccgtcagccg caattagcgg ccaggtgaag tatggttctt
     8221 gcaggttgaa catcagcgcg ctcttacctt tcgctttcag ttctttatcc agcgccggga
     8281 tctcttccca ggtttttggc gggttcggca gcagatcttt gttataaatc agtgataacg
     8341 cttcgacagc gatcgggtaa gcaatcaact tgccgttgta acgtacggca tcccaggtaa
     8401 acggatacag cttgtcctgg aacgctttgt ccggggtgat ttcagccaac aggccagatt
     8461 gagcatagcc accaaagcgg tcgtgcgccc agaagataat gtcagggcca tcgccagttg
     8521 ccgcaacctg agggaatttc tcttccagtt tatccggatg ctcaacggtg actttaattc
     8581 cggtatctgt ctcgaatttc ttaccgactt cagcgagacc gttatagcct ttatcgccgt
     8641 taatccagat taccagttta ccttcttcga ttttggcgag agccgaggcg gaaaacatca
     8701 tcgtcgttaa tgcggataat gcgaggatgc gtgcacctgt ttttattttc ataatctatg
     8761 gtccttgttg gtgaagtgct cgtgaaaaca cctaaacgga ctctagtttc tttatacggc
     8821 aacctctttc catcctcctt gcccctacgc cccatcgttg ctttgtgtga tctctgttac
     8881 agaattggcg gtaatgtgga gatgcgcaca taaaatcgcc acgatttttg caagcaacat
     8941 cacgaaattc cttacatgac ctcggtttag ttcacagaag ccgcgttctc atcctcccgc
     9001 ctcctccccc ataaaaaagc cagggggtgg aggatttaag ccatctcctg atgacgcata
     9061 gtcagcccat catgaatgtt gctgtcgatg acaggttgtt acaaagggag aagggcatgg
     9121 cgagcgtaca gctgcaaaat gtaacgaaag cctggggcga ggtcgtggta tcgaaagata
     9181 tcaatctcga tatccatgaa ggtgaattcg tggtgtttgt cggaccgtct ggctgcggta
     9241 aatcgacttt actgcgcatg attgccgggc ttgagacgat caccagcggc gacctgttca
     9301 tcggtgagaa acggatgaat gacactccgc ctgcagaacg cggcgttggt atggtgtttc
     9361 agtcttacgc gctctatccc cacctgtcag tagcagaaaa catgtcattt ggcctgaaac
     9421 tggctggcgc gaaaaaagaa gtgattaacc agcgagttaa ccaggttgcg gaagtgctgc
     9481 aactggcgca tttgctggat cgcaaaccga aagcgctctc cggtggtcag cgtcagcgtg
     9541 tggcgattgg ccgtacgctg gtggccgagc caagcgtatt tttgctcgat gaaccgctct
     9601 ccaacctcga tgctgcactg cgtgtgcaaa tgcgtatcga aatctcccgt ctgcataaac
     9661 gcctgggccg cacaatgatt tacgtcaccc acgatcaggt cgaagcgatg acgctggccg
     9721 acaaaatcgt ggtgctggac gctggtcgcg tggcgcaggt tgggaaaccg ctggagctgt
     9781 atcactatcc ggcagaccgt tttgtcgccg gatttatcgg ttcgccaaag atgaacttcc
     9841 tgccagtaaa agtgaccgcc accgcaatcg atcaagtgca ggtggagctg ccgatgccga
     9901 atcgtcagca agtctggcta ccggtcgaaa gccgtgatgt ccaggtggga gccaatatgt
     9961 cgctgggtat tcgcccggag catctactgc cgagtgatat cgctgacgtc atccttgagg
    10021 gtgaagttca ggtcgtcgag caactcggca acgaaacgca aatccatatc cagatccctt
    10081 ccattcgtca aaacctggtg taccgccaga acgacgtggt gttggtagaa gaaggtgcca
    10141 cattcgctat cggcctgccg ccagagcgtt gccatctgtt ccgtgaggat ggcactgcat
    10201 gtcgtcgact gcataaggag ccgggcgttt aagcacccca caaaacacac aaagcctgtc
    10261 acaggtgatg tgaaaaaaga aaagcaatga ctcaggagat agaatgatga ttactctgcg
    10321 caaacttcct ctggcggttg ccgtcgcagc gggcgtaatg tctgctcagg caatggctgt
    10381 tgatttccac ggctatgcac gttccggtat tggctggaca ggtagcggcg gcgaacaaca
    10441 gtgtttccag actaccggtg ctcaaagtaa ataccgtctt ggcaacgaat gtgaaactta
    10501 tgctgaatta aaattgggtc aggaagtgtg gaaagagggc gataagagct tctatttcga
    10561 cactaacgtg gcctattccg tcgcgcaaca gaatgactgg gaagctaccg atccggcttt
    10621 ccgtgaagca aacgtgcagg gtaaaaacct gatcgaatgg ctgccaggct ccaccatctg
    10681 ggcaggtaag cgcttctacc aacgtcatga cgttcatatg atcgacttct actactggga
    10741 tatttctggt cctggtgccg gtctggaaaa catcgatgtt ggcttcggta aactctctct
    10801 ggcagcaacc cgctcctctg aagcgggggg ttcttcctct ttcgccagca acaatattta
    10861 tgactatacc aacgaaaccg cgaacgacgt tttcgatgtg cgtttagcgc agatggaaat
    10921 caacccgggc ggcacattag aactgggtgt cgactacggt cgtgccaacc tgcgtgataa
    10981 ctatcgtctg gttgatggcg catcgaaaga cggctggtta ttcactgctg aacatactca
    11041 gagtgtcctg aagggcttta acaagtttgt tgttcagtac gctactgact cgatgacctc
    11101 gcagggtaaa ggtctgtcgc agggttctgg cgtcgcgttt gataacgaaa aatttgccta
    11161 caatatcaat aacaacggtc acatgctgcg tatcctcgac cacggtgcga tctccatggg
    11221 cgacaactgg gacatgatgt acgtgggtat gtaccaggat atcaactggg ataacgacaa
    11281 cggcaccaag tggtggaccg tcggtattcg cccgatgtac aagtggacgc caatcatgag
    11341 caccgtgatg gaaatcggct acgacaacgt cgaatcccag cgcaccggcg acaagaacaa
    11401 tcagtacaaa attaccctcg cacaacaatg gcaggctggc gacagcatct ggtcacgccc
    11461 ggctattcgt gtcttcgcaa cctacgccaa gtgggatgag aaatggggtt atgactacac
    11521 cggtagctct tcaaccaacc catactacgg caaggctgtg tcggctgatt tcaatggcgg
    11581 tagcttcggt cgtggcgaca gcgacgagtg gaccttcggt gctcagatgg aaatctggtg
    11641 gtaatagcaa aaaactgggc cggataaggt gtttgcaccg catccggcaa tcaatgcctg
    11701 atgcgacgct gttgcgtctt atcaggccta caaccgctgc cgaaagtagg ccggataagg
    11761 cgtttacgcc gcatccggca taaaaacagc tttaaggggc gaaagcccct ctgattatcg
    11821 ggtttagcgc gctattgcct ggctaccgct gagctccaga ttttgaggtg aaaacaatga
    11881 aaatgaataa aagtctcatc gctctctgtt tatcagcagg gttgctggca agcgcacctg
    11941 gaatcagcct tgccgatgtt aactacgtac cgcaaaacac cagcgacgcg ccagccattc
    12001 catctgccgc gctgcaacaa ctcacctgga caccggtcga tcaatctaaa acccagacga
    12061 ctcaactgtc gaccggcggc caacaactga acgtttccgg catcagtggt ccggttgctg
    12121 cgtacagcgt cccggcaaac attggtgaac tgacactgac gctgaccagc gaagtgaaca
    12181 aacaaaccag cgtatttgcg ccgaacgtgc tgattcttga tcagaacatg accccatcag
    12241 ccttcttccc cagcagttat ttcacctacc aggaacccgg cgtgatgagt gcagatcgtc
    12301 tggaaggcgt tatgcgcctg acaccggcac tggggcaaca gaaactctat gtactggtct
    12361 tcaccacgga aaaagatctc cagcagacaa cccaactgct cgacccggca aaagcctatg
    12421 ccaaaggcgt aggtaactcg gtgccggata ttcctgaccc gattgcccgt cacaccactg
    12481 atggtttgct gaaactgaaa gtgaaaacga actccagctc cagcgtgttg gtaggaccac
    12541 tatttggttc ttccgctcca gctccggtta cggtaggtaa cacggcggca ccagctgtgg
    12601 ctgcacccgc tccggcaccg gtgaagaaaa gcgagccgat gctcaacgac acggaaagtt
    12661 attttaatac cgcgatcaaa aacgctgtcg cgaaaggtga tgttgataag gcgttaaaac
    12721 tgcttgatga agctgaacgc ctggggtcga catctgcccg ttccaccttt atcagcagtg
    12781 taaaaggcaa ggggtaatta cgctccacag tgctgatttt gcaacaactg gtgcgtctcc
    12841 tggcgcacct ttttttctcc tcctgtacta ttgttgcgtt tttgttgcgc attagttcac
    12901 ttaatttgct ttacatctcc cgtaaatatt tttctgcgat acaatgcctt tacgttatgt
    12961 aacggagagt tcggcatgtc acaccccgcg ttaacgcaac tgcgtgcgct gcgctatttt
    13021 acagagatcc ctgccctgga gccgcaactg ctcgactggc tgttgctgga agattccatg
    13081 acaaaacgtt ttgaacagca gggaaaaacg gtgagcgtga cgatgatccg cgaagggttt
    13141 gtcgagcaga atgaaatccc cgaagaactg ccactgctgc cgaaagagtc tcgttactgg
    13201 ttacgtgaaa ttttgttatg tgccgatggt gaaccgtggc ttgccggtcg taccgtcgtt
    13261 cctgtgtcaa cgttaagcgg gccggagctg gcgttacaaa aattaggtaa aacgccgtta
    13321 ggacgctatc tgttcacatc atcgacatta acccgggact ttattgagat aggccgtgat
    13381 gccggactgt gggggcgacg ttcccgcctg cgattaagcg gtaaaccgct gttgctaaca
    13441 gaactgtttt taccggcgtc accgttgtac taagaggaaa aaaatatgga gtggagtctg
    13501 acgcagaata agctgctggc gtttcatcgc ttaatgcgta cggataagcc aattggcgcg
    13561 ttactgctgc tctggccaac attatgggcg ttgtgggtgg cgacaccggg cgttccccag
    13621 ctctggatcc tggcggtgtt tgtcgcgggc gtctggctga tgcgcgctgc tggttgcgtg
    13681 gtgaatgatt atgctgaccg caagtttgat ggtcatgtta agcgcacggc gaaccgacca
    13741 cttcccagcg gcgcggtgac agagaaagaa gcgcgtacgc tgtttgtagt gctggtactg
    13801 atttcatttt tactggtgct gacgctgaat acgatgacta ttctgttgtc aattgccgcg
    13861 ctggcgctgg cgtgggtata cccgtttatg aagcggtata cccatctacc gcaagtggtg
    13921 ctgggcgcgg cgtttggctg gtcgattcca atggcttttg ccgctgtgag tgagtcggtg
    13981 ccattgagtt gctggttaat gttcctcgcc aatattctct gggcggtggc ttacgacacg
    14041 cagtatgcga tggttgaccg cgatgatgat gtgaagattg gcattaaatc caccgcaatc
    14101 ctgttcggcc aatacgataa attgattatt ggtattttgc agattggcgt actagcgctg
    14161 atggcgatca tcggtgagtt aaatggctta ggctggggat attactggtc aattctggta
    14221 gctggcgcgc tgtttgttta tcaacaaaaa ctgattgcca accgcgagcg tgaagcctgc
    14281 tttaaagcat ttatgaataa taactatgtt ggtctggtac tgtttttagg gctggcaatg
    14341 agttactggc atttctgatg atgtaaaaaa gccggatgat catccggctt tcttctgggt
    14401 tgcctgatgc gctgcgcttc tcaggcctac acaacatatc gcaatttatt gaatttgcag
    14461 atcatggtag gccgaataag gcgtttgcgt cgcatccggc aattctcact gattaccctt
    14521 cgccctgcgt cgcactctca atcgtcaaac gcacgtctga tgtaatcaac tccgccagca
    14581 actgatatac cttcatcgtt tctgccggtt cggcgtcgcc gctatcgctg atataccctt
    14641 catcacgcag cgtcagcacc agagaactga acaccgcctt gtcgaagaat tccggcgcgt
    14701 tgataccgtg cagcacggag agacgttgcg cgacggtgcg gctctctttt tccagcgtac
    14761 cgcggttgat cgacgggttg gcactcaaca gccagaaggt gatggcataa cgttgcagcg
    14821 tttcgcgcgc gcctgcggcc agcagctgta gcgtgcgaga atgcgccggg ttgatatgca
    14881 actcatcatc ttgcagggta atcagcccct gacgttgcat ctcatttgcc agcgcatcaa
    14941 taacgtccgg taactcgtcg cgatcccagc gcaggaacag ttccgctttc agcattgggt
    15001 aaagcacatt gacgtgctcc atcaatacgt cgcgggagat gtggcgatgc tgggtgacaa
    15061 ttgccgccat cagcgaaggc agcaccaaca tatgcgcaat gttgttgcga tagtaggtca
    15121 tcagcaccgc ttgctcgcgc ggcagaatga tgatgtcgcc gatagtgtct ttctcgactt
    15181 caaacttgtt catttgcagc gcgtgatcga taagctcgct ggcgctggct gaaggaacgg
    15241 tagagtccgt ggagtaaggc acgttgcgca tcagatccag gtagcagttg agttgcttgg
    15301 ttaactgctc gcgggtgagt gagcgctgac gtgacgccag aagcgcagta cagcacaggt
    15361 tcatggcgtt cgccgcgcct gcgttgttaa tgcgtaccat cagatcggca gcaatattat
    15421 tgaccgtcgg cgttaaccag gccggacgca ccgcttcgat gggatcgata gattcacgcc
    15481 agtctggtac gtgctggtta aggtaggtca tcaacggcat tggttcaccg aagttgacgt
    15541 aaccctgacc gagattacgc agcttgctta aaccgcgcag catctgcggc aggctctctt
    15601 tctctttcgt cgcaccgcgc agttctttgg cgtaagtacc cacttccatg acgtgctcat
    15661 aaccgatata gatcggaatc agcgtaatcg gacgagtgcc gccacgcagc atcgcctgaa
    15721 tggtcatcga tagcgtaccg gttttcggat ccagcaaacg ccccgtacgg gaacgaccgc
    15781 cttccacgaa atactcgacg gaataaccac ggctgaacag ttcgccgaga tactcacgga
    15841 aaacggtgga ataaagttta ttgcctttaa acgtacggcg aataaagaac gcccccagac
    15901 ggcggaaaat cggcccggcc ggccagaagt tcaggttgat cccggcggcg atatgcggcg
    15961 gcaccagccc ctggtgatac agcacgtaag aaagcagcag gtagtccatg tgactgcggt
    16021 ggcaaggcac atacactagc tcatggccgt cgtgggccag ctggcgaacg cgctcggcgt
    16081 tatggacgtt gataccctgg taaagtcggt tccaggtgaa gcccagaatg cggtcagtca
    16141 ggcggatcat ctcgtaagag aaattcgccg caatctcttc catcagcgca atcgcgttct
    16201 gctgcgcttt ttcatgggag atttttttgc tgcgtgcttc atcttctacc gctttcgcaa
    16261 tggcgcggga ggcgagcagc ttattaaaca gatcctgacg agcaggcaga cgtggaccca
    16321 cggcagccag acgttgacgg gcaaagtgca tacgcgccac gcgcgccagt ttctgagcga
    16381 tagttttatc cgtgccgtgt tcatccgcca tacggcgcag tgaaactgac ggcgagaaac
    16441 gcacaaaact gtcgcgaccg agccacagta cagcgaaaaa tttctgtacg ccgttaagca
    16501 tacgcagcgg cgggttcact tcgccttttt cacgccccgg cgcgcgacca aacatcaccg
    16561 atactggcac catctgcaca tccagatttg ggttgctgcg gtgcaaatcg agatagtcgt
    16621 ggaacagctt aatagactct tctttcggcg tgtaataggt gaacacacgc ggcccgccgt
    16681 gaatgaacac atagcgcggc agtagcgtgc cgtcgatttc cagcggttct aacgggtcag
    16741 gcaggtcatg tgccagacac tgggcgcgca acgtcagcaa gtcagctttc gagttgtacg
    16801 gtaaaacgta cataattgga cgagaggtat ccagccccag ttccggggca ggatccgccg
    16861 gaatagactt gctttttacc aggatgctta atggtaaatt cagtaatttg tagtaaattc
    16921 gtggccagcc ggacataaac gatgtaaagc ctctggttaa taatgcaaat gcgcggcaag
    16981 gatagcagaa agtcatggga aattctgtgg tatccgctca tgtttcgcgc ggcgttacgc
    17041 aaacccgaat catcggattt aacggtacac tgatattgac gctcataatg taaaaaggtt
    17101 ctttcaatgg ccaataatac cactggattc atccgaatta ttaaagctgc tggctattcc
    17161 tggaaaggtt tacgcgctgc atggatcaac gaagcggcat tccgtcagga aggcgtagcg
    17221 gtattgttag cggtggttat cgcctgctgg ctggatgtgg acgcgattac ccgcgtgctg
    17281 cttatcagct ccgtgatgct ggtgatgatt gtggaaattc tcaatagcgc catcgaagct
    17341 gtggttgacc gaattggctc tgaataccat gagctttccg gacgcgctaa agatatggga
    17401 tctgccgcag tgctgattgc cattatcgtc gccgtgatta cctggtgcat cctgttatgg
    17461 tcgcattttg gataaccctt ccagatttcg ataaatctct ggtttattgt gcagtttatg
    17521 gttccaaaat cgccttttgc tgtatatact cacagcataa ctgtatatac acccaggggg
    17581 cggaatgaaa gcgttaacgg ccaggcaaca agaggtgttt gatctcatcc gtgatcacat
    17641 cagccagaca ggtatgccgc cgacgcgtgc ggaaatcgcg cagcgtttgg ggttccgttc
    17701 cccaaacgcg gctgaagaac atctgaaggc gctggcacgc aaaggcgtta ttgaaattgt
    17761 ttccggcgca tcacgcggga ttcgtttatt gcaggaagag gaagaagggt tgccgctggt
    17821 aggtcgtgtg gctgccggtg aaccgcttct ggcgcaacag catattgaag gtcattatca
    17881 ggtcgatcct tccttgttca agccgaatgc tgatttcctg ctgcgtgtca gcgggatgtc
    17941 gatgaaagat atcggcatta tggatggcga tttgctggca gtgcataaaa ctcaggatgt
    18001 acgtaacggt caggtcgttg tcgcacgtat tgatgacgaa gttaccgtta aacgcctgaa
    18061 aaaacagggc aataaagtcg aactgttgcc agaaaacagc gagtttaaac caattgtcgt
    18121 tgaccttcgt cagcagagct tcaccattga ggggctggcg gttggggtta ttcgcaacgg
    18181 cgactggctg taacatatct ctgagaccgc gatgccgcct ggcgtcgcgg tttgtttttc
    18241 atctctcttc atcaggcttg tctgcatggc attcctcact tcatctgata aagcactctg
    18301 gcatctcgcc ttacccatga ttttctccaa tatcaccgtt ccgttgctgg gactggtcga
    18361 tacggcggta attggtcatc ttgatagtcc ggtttattta ggcggcgtgg cggttggtgc
    18421 aacggcgacc agctttctct ttatgttgtt gctgttttta cgcatgagca ccaccgggct
    18481 gactgcacag gcttatggtg ccaaaaatcc tcaggcatta gcccgtgcgc tggtgcaacc
    18541 gttgctgttg gcgttggggg ccggggcgtt aattgcgctg ctgcgtacgc cgattatcga
    18601 tctggcgctg catattgttg gtggtagtga agcagtcctg gaacaggcgc gacgctttct
    18661 tgaaatccgc tggttaagcg caccggcgtc gctggcgaat ctggtattac ttggctggtt
    18721 actcggcgtg caatatgccc gtgcgccagt aattttgtta gtggtcggca atatcctcaa
    18781 cattgtgctg gatgtctggc tggtgatggg gctgcatatg aacgtgcagg gcgcggctct
    18841 ggcgacggtt attgcggaat atgcaacatt gctgattggt ctgctaatgg tgcgtaaaat
    18901 tctcaaacta cgcggaatct ccggcgaaat gctgaaaaca gcctggcgag gaaacttccg
    18961 tcacttgctg gcgcttaacc gcgatatcat gctgcgctcg ctgttgctgc aactctgttt
    19021 cggcgcgatc actgtacttg gcgcgcgact ggggagtgac attatcgctg ttaacgcggt
    19081 tctgatgacg ctactcacct ttaccgccta tgcgctggat ggttttgcct acgcggttga
    19141 agcacattcc ggtcaggcat acggtgcgcg cgacggtagt caattgctgg atgtctggcg
    19201 ggcagcgtgc cgccagtcgg ggatcgtagc gttactgttt tcggtggttt atttgctggc
    19261 aggggaacac atcattgcgt tgctgacgtc gttaacccag attcagcagc tggctgaccg
    19321 ctatcttatc tggcaggtga ttttgccgtt ggtcggcgtc tggtgttatc tgctggacgg
    19381 catgtttata ggcgcaacgc gtgccgccga aatgcgtaac agtatggcgg tggccgccgc
    19441 aggttttgca ctgacgctcc ttacgctgcc gtggctgggg aatcatggtt tgtggctggc
    19501 gttaaccgtc tttctggcgt tacgcgggct ttctctggcg gctatctggc ggcgtcactg
    19561 gcgtaacgat acctggtttg tcgcaacgtg acggttaaaa attctgaata aataatccta
    19621 agccaaattg ctgactacac ttaatctcac gttcagaaga aaagtgaacg tactctcatt
    19681 cacaacctaa cgatgaggtc ttgattatga ataaagatga agccggcggt aactggaaac
    19741 agtttaaagg taaagtgaaa gagcaatggg gcaaactgac cgatgatgat atgacgatca
    19801 ttgaaggaaa acgtgatcag ttagtgggta aaatccagga acgctacggt tatcagaaag
    19861 atcaggcaga aaaagaggtt gatagttggg aaaaacgcca tgattaccgc tggtaattaa
    19921 tccctcctgc ccgacatgta caaggatgta caccctttta gcgaggtttc tttttcacct
    19981 gaatagagtg gtcatgatgg cactgttcag gatgacgaca cgcttctact tctacacatg
    20041 ctgaacataa tccatgtgct tcaataacat tatgccgcag ggcaaaaccc atttttgccg
    20101 ccagcgtatg catgatgtct tccacgcctt cagcacattc ttctttcacc gcgccgcagc
    20161 gatcgcaaat aaacatggct gaagtatggg tgggctgatc gaacagatga cagagcacat
    20221 aactgttggt ggattccacc ttatgcacaa aaccttgttc aagcagaaac tccagcgcgc
    20281 gataaaccgt tggcggcttg gcttgcggtt cagcttcgcg cagcaaatca agcagatcat
    20341 aagcgctgat agcgccatcc tgcagactca tcaggcgcaa cacttcgaga cgctgtgggg
    20401 tcaggcgcac attacgctgt gcgcagattt tttcagcctg cgctaataac tcctgcgttg
    20461 tggtcttttc cattgggcac ctcgacagtg gggttaagaa tgtccttact ttaccatagt
    20521 tctcacttaa acgccgagaa cagggtgtca tggctgtcac attgcctgat cgcatattcg
    20581 ttatgctata gtagcgcccc caatttacca gatgcttaaa aagtcgccat gccagcagaa
    20641 tcacacactg tttatcccgc ttaccgtttc tcgattgccc cgatgcttga ctggacggac
    20701 agacattgcc gttatttcct gcgtctgctt tcccgcaata cgttgctgta taccgaaatg
    20761 gtgaccacag gggcgattat tcacggtaaa ggtgattacc tggcgtacag tgaagaagaa
    20821 catccggtag cgttgcaact cgggggtagc gatccggcgg cgctggcaca gtgtgcgaag
    20881 ctggcagaag cgcgcggata tgatgagatc aacctgaatg tcggctgccc gtctgaccgg
    20941 gtgcagaacg gcatgtttgg tgcgtgtctg atgggtaatg cgcagctggt tgccgactgc
    21001 gtgaaagcga tgcgcgatgt ggtgtcgatt ccggtgacgg tgaaaacgcg tattggcatc
    21061 gatgatcagg acagctatga atttctctgc gatttcatca acaccgtttc cggcaaaggt
    21121 gagtgtgaga tgttcatcat ccacgcacgt aaagcctggc tttctgggtt aagtccgaaa
    21181 gaaaaccgtg agatcccgcc gctcgattat ccgcgtgtgt atcaactgaa gcgtgacttt
    21241 ccgcatctga cgatgtcgat taacggtggt atcaagtcgc tggaagaagc taaagcgcat
    21301 ttgcaacata tggatggcgt gatggtcggg cgcgaggcgt atcagaatcc ggggattctg
    21361 gcggcggtag accgagagat ttttggttcc tcggataccg atgccgatcc ggtggcggta
    21421 gtgcgcgcca tgtatccgta cattgagcgt gaactcagcc aggggacgta tctcggccat
    21481 attacccggc atatgctggg cttgttccag ggtattcctg gcgcgcggca gtggcgacgt
    21541 tatttaagtg aaaatgccca taaagcgggt gcagacatta atgtgctgga acacgcgctc
    21601 aaactggtgg cggataagcg ttaacttttc accaaaaagt agtcaaattc accacgccct
    21661 gcgcaccgtc gcggggcgtt ttgctgttaa atcaatagat tatttttggc atgattcttg
    21721 taatgccagc aagagatttc atatttggga gagcatcatg ctggaactac tttttgtaat
    21781 tggctttttt gtcatgctga tggtcactgg tgtttcgttg ctgggtatta tcgccgcgct
    21841 gattgtggcg acggccttta tgttcctcgg cggtatgctg gcattgatga ttaagttgct
    21901 gccgtggctg ctgttggcgg ttgcggtggt gtgggtgatc agggcgatta aagcaccaaa
    21961 agtgccgaaa tatcagcatt atgaccgctg gcgttactaa gggattgtgc ggatgatcac
    22021 aacctaaggt tttatcctta gaacaaaata ggaattgata atcaaatctg tcactattgc
    22081 gtgtcaaaca aattcatcga gctgtaccct acatacagcc gaactataaa agaaagggct
    22141 tcccaggtgg aagcccaatt tcttttatgg aatcaacagg ctggaaccct gcgtcgcccg
    22201 gctttccaga atctcatgcg cacgccgcgc atccttcagc ggatatttct gctgctcggc
    22261 gacatcgacc ttaatcacac cgctggcaat caaagagaac agttcattac tggcctcggt
    22321 taattcctcc cgcgtggtga tatagccttg cagggaaggg cgtgtcacat acaacgagcc
    22381 tttctgattg agaatgccta agttcacacc ggtaaccgca cctgatgagt tgccaaaact
    22441 caccattaag ccgcggcgtt gcaggcaatc cagcgaccgt tcccaggtgt ctctgcccac
    22501 tgaatcgtac accacgcgca ctttcttgcc accggtgatc tcttttaacc gctcgaccag
    22561 attctcttca cgatagttaa taacctgcca tgcgcccgct tttagcgcac tctgcgcttt
    22621 ttgcgcggtt cccacggtgc cgataagttt cgcgcccagg gcttttgccc actggcaggc
    22681 aatcaagcca acgccgccag ccgctgcgtg gaacaggaac tgttcatcgg gtttaatttc
    22741 ataggttttg cgcagcagat aataaaccgt taagcctttc aggaaggatg ccgcagcttg
    22801 ctcaaaagaa attgccgcag gcagaatcgc cgctttatcc gcattaatgt tatgcataga
    22861 gctgtaagcg cctaatgccg attgtgcata gactacgcga tcgcctgcct taatatgctt
    22921 tacaccactg ccgactttac tcacgatgcc tgccgcttcg gtgcctaatc cgctgggtag
    22981 cgatggtggc gggtaaaggc cgctgcggat atacgtgtcg ataaaattga tgccgatggc
    23041 tttatttttg acctggattt cattctccgc cggatcggca ggagtgaact ccacggcctg
    23101 aagtacttcc gggccaccgt gcttgtgaaa ttcgatgcgc gttgccattt aaactccttt
    23161 actgagcgac atatctgttt taagcctaac agtagtcgcc aggccgcgcg gggatttttt
    23221 ccgggctgcg gtcacaaccc ggaatatctc tgatcagtat tgaaacggcg tcgccgcaat
    23281 ccagttttgc cactggcgct caaagttggt tagcgttaat acgccccgtt cacggcacgg
    23341 gcgagtccag gtttggtaat cattagcttc ccgacaaaca caaatcatat attgcctggc
    23401 atcctgattt tctggccgaa aacgccaggc caggagtggc ccacctggtg tttgcggtgc
    23461 aataccggct tcgccatcat agatagccct ggcaccacga ctcccaccac cgttttcgac
    23521 ataacattgt aaagaacgca gtacagccag cgccagttgc gcactttgcc gccactggaa
    23581 agattgtgcc aggctgtttt catcgcattg aataccttcc cggcgaatat cttcacaaag
    23641 tgttgccagg gtttgcgcgg cagtgttgat cccctgtgta tgacacaaaa ttgcggcatg
    23701 gtggctcatg gtattttgta tcgtttctcg cacgtcgtca cgtagcagcc cctgattcag
    23761 gtactgttgc gcctcattaa tcgtactgag aatttgttgc ctctcgagag gccggtcctg
    23821 gcttttctga tgagcaatat gtaatgcaca gcggcgggcg aaaacttgcc cggcattgag
    23881 cgctgctcca cctgggcgcg tgacaccatg tgttccggca ttttctccgg cagcataaca
    23941 accagttagc gatgtacgtc cccaaatatc aacttcaatt ccgccattaa gatgttgatt
    24001 atttagcgta aacttgagcg gtgaggctgt gagatcatga ccgtgcatct gatacaaacg
    24061 aatagccaga ggattcatct gtgttaatct cgccagcggt tgtgccaaca gggcgtgatt
    24121 ttgttgcaga tagtcgatga catcatcatc cagttcttgc aggttgaatg acgttccgtc
    24181 cgctgcgggt tctggttccc gtagaaaatc aagaaaaaca tcgcgccctt tttgagtctc
    24241 ttcataaact gcgacatcca gcaggctgga gccatattcc agcattcgtt ccgcgtgaaa
    24301 cggccactgg taacctttac gaaatattgc ggaggccagc atacgagtcg tggggtaata
    24361 agctgccaga aaattatact gacgcccgga gtggtcctgc gaatagatgc gcggcatcgc
    24421 ctgcatgtaa gtaccggaaa gattccacgg aaactggctg cgcggtgtac cgataccaaa
    24481 ctgactttcc gtaagattaa ccagagttat acctgcttcc agtgccatgc ccaacgcact
    24541 aaaacagttc accggataaa cactgtcacg ataaagttca cctggtccgc ccgtggctaa
    24601 aaccagatgc tggcagcgaa tcacgaccat tcgccaggga ttatcccgac aggctttatc
    24661 gatggcgatc acccccacga ctcgtcgatt ttccccttca ccactggtca ggatatgaat
    24721 cgccgttgtg tgatcgcaaa gaggaatatt aaggcgcatt gcctcctggg ccagcacctt
    24781 caccatcagg cgcgatgttc gaggaccgca gctagtggca cggccaaatt catcgtgatc
    24841 ggtttgataa cgcaacgttg cgccaaagag atcttcaggc agatccagac ccagatattt
    24901 cagtacttca caggtgttca ccgatccgac cgcctcaaca tatgcggtgt catgatccat
    24961 cgcaccacct gccgccaggg tgttacttaa tgtcagaaaa tggtcaccat ttgcacgcgt
    25021 attcgcggtg tgaagcgttt gtttatcgga accagaacag gcggaagtgc cccagaaggc
    25081 tttgctggag gcgaccatta catcgacatt ttgccgtttc agctcgacgg cagctcgcca
    25141 tcctgccgcg ccactgccca gaaccagcgt gttgcagcag tactctggta aaatggtacc
    25201 gttaaggtcg atactaccgc tggcttgagc cagcggtgcg taatggggca ttttgatatc
    25261 agtgagcgcc gttagcgttt cgtcaatcat tgcgtcccac ctatttcatc agactttcga
    25321 ttaagccgct cattgccata tagcccatga ttagcgtaaa tacgcttatg gcaataaaca
    25381 tgacgttttc ccagcgtgag caacgatatt tgcccattaa cgaaggcttg ttcgccagcc
    25441 agaacataca taaaacagtc agcggcagaa taatggcgtt aaaggcctgg ctggcaatca
    25501 tcaccaatac cgggcgtgcg tggaacagcg gcacaaccag acaaagcaga gacataaccg
    25561 tcatcaggat acggtaacct gggctaactt cgttgctggt atcacgccag tcagccagca
    25621 gccagggcgt taacagcaaa tttgggaact ggctggaaag cccggctgca acaataccaa
    25681 taacaaagag ccccacggca gctgagccag caataggttc gagaataatg accatttgcg
    25741 aggcattatt taaacctgtg ccagtttggt acaacgtacc cgcagccgca gccatcaccg
    25801 ccgcactgat gatgaacatc attcctgcac tgacaatggc atcacgattt tgatgaggca
    25861 aattgtccac accccaacca ttacttttta ccagggtcgt gcgcataaga aacattgctg
    25921 gtgctacggt ggtacccacc atgccagaaa ttatcagaaa tggcgtactg ttatcgccag
    25981 cgacaggatc gggaacagac ggaataaatc cggccagcag atctgcgacc ggcggcatca
    26041 taatgaaaaa attaatcaca aacgccagcc ccataatgcc aaccagcgcg gagaggattt
    26101 tgtcgaaccc ggcgttgctg ttggtccaga gcatcacgtt aatcataatg accatcgcga
    26161 tagcccaggc gagagctgag ataccgccac taaaccaggt cgtggaccat gttgccatta
    26221 catccgccag aatacccatt acccccataa tggaaccgct cacggcaacg gttaacccga
    26281 tcagaaacaa catcgcaacg ccgggatgga tatggcgacg aaatgcagtc aacgcgcgtt
    26341 caccagtgac ggaagcataa cgaccataaa tacggattaa aaaccaggta acaatgcaag
    26401 aaagcgccac ggcccacatg agcgagacac catagttagc acctgcttta gccatagtgg
    26461 tgatgctgcc tgttccgatg ctgtagccaa taagaaagat gccaggtgca aaggccgcga
    26521 gttttgtcat tattcgccga aataaagaag gcgtttgggg aacagaggtt gtcatattca
    26581 ttatctttct ttccttgtaa gaggcgatca gcgtgcctga aagtgcggtt gacgcttctc
    26641 agtaaatgcc tgactgcctt catgagcatc ttcagtcgtg gcggaaaaag cgccagccag
    26701 tgcttcaagc gtcgaggcca cagcaatacc ttctccggca tcaataacct gcttggcgat
    26761 ctgcactgca acgggagcac gctgggcgat acgttgtgcc agttgcagtc cggcgtcgtg
    26821 taacgcctcc ctggcgacaa cgcgattcac cagcccgcag gacagtgccc gctgtgcatc
    26881 cagtggttca ccggtaaata ccagttcttt caccagtgag cgcccaagca gtgccgtcag
    26941 ccgctgcgtt cctgaccagc caggcactgt cgccagcccg gtttctggta gtgccatttg
    27001 tgcttcggcg ctacaaatac ggatatcggc ggtagcagcc agttccagac caccgccata
    27061 agccaggccg tgcaacaggg cgattacggg ctgtggcagt tgtgccagag catcaaatgc
    27121 ctgatgaccg cggcggatcc agttgcgcca catatcgagt gcagaaagag aggtccagcg
    27181 atgaatatcc gcgccgacac aaaacgcttt ttcactggca ctggtcagca gtactacgcg
    27241 gatctccgaa tcagtttcca acgtgtggca atgcgccagc agttgttcga gcatctctgg
    27301 ggttaatgcg ttgagtttcg ccgggcgatt aatcaccaaa gtggcaatga aaccgttgcg
    27361 ctggaggtca atggtgccgt tattcatggt gacttccttg ttgaaaatag aggggattca
    27421 ttagtttgag atcgtctgcc agacggagtg gggcatcgct ttgggcgaga atgtcgcgtt
    27481 caggatcaat acctggggca atttctgtga ccagtagccc gtctggggtt aattccagaa
    27541 cgcagcgttc agtaatgtac tgtacttgtt gcccgagtct tattgcgcgt ttgccggaga
    27601 acgtcacgtg ggcgacgtcc tgtaccagtt ttttggcttt gccttcttta atgatacgca
    27661 gctgaccctc ttccagttgc agttgggcac cggcattgaa aaagccagag aaaataatgc
    27721 gtttcgcatg tgaggtgata tcaataaatc caccgcatcc ggcagtgaca tgtggacgag
    27781 cggggagatg tgagacgttc actgagccat ctgcgccgat ttgtaaaaac gacatcagag
    27841 tgagatcaaa accaccaccc tggaaatagg tgaactgctg gggagagggc aaaaatgctt
    27901 ctgcattaga ggcacaaccg aactgaaatt ccagcagtgg aactccgcca atcgcgccct
    27961 gttccagcag ccaggtgacg tcaccgtggc gaccttgttc cagtaaaatg cgtggtacgt
    28021 tggcagaaat accaaagcca atatttaccg catctccact ttgtagttct tgcgcgactc
    28081 gctgggcaat aactcgcgcc gggccatgtt ccatataacg aaaggctgac agcggacgac
    28141 tgatttcgcc ggaaatggca ggttcatatt gcgtttgtgt cgtttgtgtt tgttccggcg
    28201 caatcacgat gtaatccacc agtacacccg gaatacgcac ctcttttggt ttcagacttc
    28261 cggcggcaac ctgtcgtttt acttgcgcaa ttatgatgcc accattattg cgaacggcag
    28321 tagcctgttc cagtggcccc agataagccc cttcatgttc ataagtcagg ttgccgcgtt
    28381 catcggcagt ggtagcgcga atgatcgcca cctgcggcac gatactgggg aaaaacagcc
    28441 agtcgtcgtt agcaaatcga acacgttcaa ccagagattg ttgcgccgct ttttcattca
    28501 ttgcaccgcc ctgcaacagg ggatccacat aggtatccat gcctgtttgt gtcagcacgc
    28561 caggacgatg agccgccgct tcccggtgca tatcaaataa gatcccggaa ggcagattcc
    28621 aggcggggat cgcgttgtca ttgatcattt gccagattaa cggaggcgtc attgatgatg
    28681 gaccggaggg gtaggagcca gcgatcactt ttgccagtaa gcccggttgc gccagatgat
    28741 cgatgccttt tacgccatac atatcaccag cggcgatagg gtggatggag gtgatgtttt
    28801 gcggatgtcc ggcacgttga aagcgttcac ctattgctgc cagcaccgca tccggacacc
    28861 ccattccact ggaggaggag acggtgacaa ctgcgccatc aacaatcaaa tctgcagctt
    28921 ccggtgcagt gatcgtgcgt
//
LOCUS       DACADF010000052        28319 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.052, whole genome shotgun sequence.
ACCESSION   DACADF010000052 DACADF010000000
VERSION     DACADF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.052"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..299)
                     /locus_tag="ISB30_002136"
     CDS             complement(<1..299)
                     /locus_tag="ISB30_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP1989766.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQM"
     gene            complement(370..2484)
                     /gene="fusA"
                     /locus_tag="ISB30_002137"
     CDS             complement(370..2484)
                     /gene="fusA"
                     /locus_tag="ISB30_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP1989767.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2581..3051)
                     /gene="rpsG"
                     /locus_tag="ISB30_002138"
     CDS             complement(2581..3051)
                     /gene="rpsG"
                     /locus_tag="ISB30_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP1989768.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3148..3522)
                     /gene="rpsL"
                     /locus_tag="ISB30_002139"
     CDS             complement(3148..3522)
                     /gene="rpsL"
                     /locus_tag="ISB30_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP1989769.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3648..3935)
                     /gene="tusB"
                     /locus_tag="ISB30_002140"
     CDS             complement(3648..3935)
                     /gene="tusB"
                     /locus_tag="ISB30_002140"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAP1989770.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3942..4301)
                     /gene="tusC"
                     /locus_tag="ISB30_002141"
     CDS             complement(3942..4301)
                     /gene="tusC"
                     /locus_tag="ISB30_002141"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAP1989771.1"
                     /translation="MKRIAFVFSTAPHGTTAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWICAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4301..4687)
                     /gene="tusD"
                     /locus_tag="ISB30_002142"
     CDS             complement(4301..4687)
                     /gene="tusD"
                     /locus_tag="ISB30_002142"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAP1989772.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4687..5409)
                     /locus_tag="ISB30_002143"
     CDS             complement(4687..5409)
                     /locus_tag="ISB30_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1989773.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5576..6388)
                     /gene="fkpA"
                     /locus_tag="ISB30_002144"
     CDS             complement(5576..6388)
                     /gene="fkpA"
                     /locus_tag="ISB30_002144"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAP1989774.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6609..6827
                     /locus_tag="ISB30_002145"
     CDS             6609..6827
                     /locus_tag="ISB30_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAP1989775.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6876..7466)
                     /gene="slyD"
                     /locus_tag="ISB30_002146"
     CDS             complement(6876..7466)
                     /gene="slyD"
                     /locus_tag="ISB30_002146"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP1989776.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7561..7761)
                     /locus_tag="ISB30_002147"
     CDS             complement(7561..7761)
                     /locus_tag="ISB30_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAP1989777.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7771..9576)
                     /gene="kefB"
                     /locus_tag="ISB30_002148"
     CDS             complement(7771..9576)
                     /gene="kefB"
                     /locus_tag="ISB30_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAP1989778.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSSASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9576..10127)
                     /gene="kefG"
                     /locus_tag="ISB30_002149"
     CDS             complement(9576..10127)
                     /gene="kefG"
                     /locus_tag="ISB30_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAP1989779.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10258..12171
                     /locus_tag="ISB30_002150"
     CDS             10258..12171
                     /locus_tag="ISB30_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1989780.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGQAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12171..13193
                     /locus_tag="ISB30_002151"
     CDS             12171..13193
                     /locus_tag="ISB30_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP1989781.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWHRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRIYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13187..13405
                     /locus_tag="ISB30_002152"
     CDS             13187..13405
                     /locus_tag="ISB30_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAP1989782.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13459..14328
                     /locus_tag="ISB30_002153"
     CDS             13459..14328
                     /locus_tag="ISB30_002153"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAP1989783.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14383..14787)
                     /locus_tag="ISB30_002154"
     CDS             complement(14383..14787)
                     /locus_tag="ISB30_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAP1989784.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15089..15721
                     /gene="crp"
                     /locus_tag="ISB30_002155"
     CDS             15089..15721
                     /gene="crp"
                     /locus_tag="ISB30_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAP1989785.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15772..17862
                     /locus_tag="ISB30_002156"
     CDS             15772..17862
                     /locus_tag="ISB30_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989786.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17929..19149)
                     /gene="argD/dapC"
                     /locus_tag="ISB30_002157"
     CDS             complement(17929..19149)
                     /gene="argD/dapC"
                     /locus_tag="ISB30_002157"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAP1989787.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVVGA"
     gene            complement(19235..19798)
                     /gene="pabA"
                     /locus_tag="ISB30_002158"
     CDS             complement(19235..19798)
                     /gene="pabA"
                     /locus_tag="ISB30_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAP1989788.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19830..20432)
                     /locus_tag="ISB30_002159"
     CDS             complement(19830..20432)
                     /locus_tag="ISB30_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAP1989789.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNKLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYLEKEGNAL
                     MQDLEEEKYLTSLEKDKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEVGESE"
     gene            complement(20422..20589)
                     /locus_tag="ISB30_002160"
     CDS             complement(20422..20589)
                     /locus_tag="ISB30_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAP1989790.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20694..21266)
                     /gene="ppiA"
                     /locus_tag="ISB30_002161"
                     /gene_synonym="rotA"
     CDS             complement(20694..21266)
                     /gene="ppiA"
                     /locus_tag="ISB30_002161"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAP1989791.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21537..22718
                     /gene="tsgA"
                     /locus_tag="ISB30_002162"
     CDS             21537..22718
                     /gene="tsgA"
                     /locus_tag="ISB30_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAP1989792.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22980..25523
                     /locus_tag="ISB30_002163"
     CDS             22980..25523
                     /locus_tag="ISB30_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAP1989793.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVARIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25520..25846
                     /gene="nirD"
                     /locus_tag="ISB30_002164"
     CDS             25520..25846
                     /gene="nirD"
                     /locus_tag="ISB30_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAP1989794.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
     gene            25973..26779
                     /gene="nirC"
                     /locus_tag="ISB30_002165"
     CDS             25973..26779
                     /gene="nirC"
                     /locus_tag="ISB30_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAP1989795.1"
                     /translation="MFTDTITKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26798..28171
                     /gene="cobA"
                     /locus_tag="ISB30_002166"
     CDS             26798..28171
                     /gene="cobA"
                     /locus_tag="ISB30_002166"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAP1989796.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6852 a         7579 c         7151 g         6737 t
ORIGIN      
        1 tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc cgggcagtct
       61 acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt gagaagtgtt gatggtgata
      121 ccacgagcct tttcttccgg cgcgttatcg atctggtcga atgcacgagc agcaccgccg
      181 taggttttag ccagtacggt ggtgattgca gcggtcagag tagttttacc gtggtcaacg
      241 tggccgatag taccaacgtt aacgtgcggt tttgtacgtt caaatttttc tttagacacg
      301 gctatattcc ttactatagt gctctcccct tcaggagaga gcacgggact ttggtattaa
      361 cccttaggct tatttaccac gggcttcaat tacggcctga gcaacgttac tcggcgcttc
      421 atcatacttc aggaattcca tagtgtatga tgcgcgacct ttggtcagag aacgcagctg
      481 agttgcatat ccgaacattt cagacagcgg tacttcagcg tggatcttaa cgccagtaac
      541 ttcagattcc tgacctttga gcataccacg acgacggctc aagtcaccga taacgtcacc
      601 ggtgttctct tcaggagttt ctacttcaac cttcatgatc ggctcaagca gaactggttt
      661 cgctttctta aagccttctt taaaggcgat agaagcagcc agtttaaacg ccagttcaga
      721 ggagtcaacg tcatggtaag aaccgaagtg cagacgaata cccatgtcta ctaccgggta
      781 gcctgccagc ggacctgctt tcagctgttc ctggatacct ttatcaacgg ccgggatgta
      841 ttcgccaggg attacaccac ctttaatgtc gttgatgaac tcgtagcctt tcgggtttga
      901 acccggctcc agcgggtaca tgtcgataac aacatgacca tactgaccac gaccaccaga
      961 ctgtttcgcg tgtttacctt caacatcggt aactttctgg cggatagttt cacggtaagc
     1021 aacctgcggt ttacctacgt tcgcttcaac gttgaattca cgcttcatac ggtcaacgat
     1081 gatgtcgagg tgcagttcgc ccatacccgc gatgatggtc tggttagatt cttcgtcagt
     1141 ccatacacgg aaagacgggt cttctttagc cagacggccc agagccagac ccattttttc
     1201 ctggtcagct ttggttttcg gttcaactgc gatggagatt accggctcag ggaattccat
     1261 acgttccaga atgatcggcg catccgggtc acacagggtg tcaccagtgg ttacgtcttt
     1321 cagaccgatt gcagctgcga tgtcgcccgc gcgaacttct ttgatctctt cacgtttgtt
     1381 agcgtgcatc tgaacgatac gaccgaaacg ctcacgtgca gctttcacgg agttcagtac
     1441 ggtatcacca gagttaacca caccggagta cacacggaag aaggtcaggt taccaacaaa
     1501 cgggtcggta gcgattttga acgccagtgc agagaacggc tcgtcatcac ttgcgtgacg
     1561 ctcagccgga gtgtctttac cgtcgtccag gataccgttg atcgcaggta cgtcaaccgg
     1621 ggatggcagg taatcaatta ccgcatccag catcgcctga acacctttgt tcttgaacgc
     1681 agaaccacag gttaccagga tgatttcgtt gttcagaacg cgctgacgca gagcaccttt
     1741 gatttctgct tcagtcagtt cttcaccacc cagatatttt tccatcagct cttcagaagc
     1801 ttcagctgcg gattcgatca ggttctggtg ccattcgtta gccagttcaa ccatgtctgc
     1861 cgggatatct tcgtattcga aggttacgcc ctggtcagcg tcgttccagt tgatagcttt
     1921 cattttcacc aggtcaacaa caccggtgaa atgttcttca gcaccaatcg ccagctgcag
     1981 cggaaccggg ttcgcgccca gacgggtttt gatctggtta acaactttca ggaagttcgc
     2041 acccatgcgg tccattttgt taacgaacgc aatacgcgga actttatatt tgtttgcctg
     2101 acgccatacg gtttcagact gcggctgaac accaccaact gcgcagtaaa ccattaccgc
     2161 accatcgaga acacgcatgg aacgttctac ttcgattgtg aagtcaacgt gccccggggt
     2221 gtcgatgatg ttgatgcgat gcggctcata ctgcttagcc ataccagacc agaatgcagt
     2281 agtcgcagcg gaagtgatgg taataccacg ttcctgctcc tgctccatcc agtccatggt
     2341 tgcagcgccg tcatgaactt caccgatttt atggtttaca ccggtgtaga acagaatacg
     2401 ttcggtagta gtggttttac cggcgtcgat gtgcgcactg ataccgatgt tacggtagcg
     2461 tgcgatgggt gttgtacgag ccatttgttt cctcgtttat cttttaggcg ttcaatttaa
     2521 gtagcccaaa gcgggctgct tactggaagc gcccgcctgg tgactaaaac tccgaaggga
     2581 ttaccaacgg tagtgtgcga acgccttgtt ggcttcggcc atacggtgaa cgtcttcacg
     2641 tttcttaact gcagtacctt tgttttctgc agcatcagaa agttcgttcg ccaggcgcag
     2701 agccatggat ttatcaccgc gtttacgagc agcttcaacg atccaacgca ttgccagagc
     2761 attacgacga accggacgga cttcaactgg tacctgataa gtagaaccac caacgcggcg
     2821 agacttaact tctacagtcg ggcgcacgtt ttcgagagct acttcgaatg cttccagttc
     2881 agatttacca gagcgctgag ccagggtctc cagcgcgctg tatacgatag attcagcagt
     2941 agatttttta ccatctacca tcaggatatt tacaaattta gccagcagtt ctgatccgaa
     3001 cttcggatcc ggcagaattt tacgctgacc aatgacgcga cgacgtggca tggaaatact
     3061 ccgttgttaa ttcaggattg tccaaaactc tacgagttta gtttgacatt taagttaaaa
     3121 cgtttggcct tacttaacgg agaaccatta agccttagga cgcttcacgc catacttgga
     3181 acgagcctgc ttacggtctt taacaccgga gcagtcaagc gcaccacgta cggtgtggta
     3241 acgaacaccc gggaggtctt taacacgacc gccacggatc aggatcacgg agtgctcctg
     3301 caagttgtga ccttcaccac cgatgtagga agtcacttcg aaaccgttag tcagacgaac
     3361 acggcatact ttacgcagcg cggagttcgg ttttttagga gtggtagtat atacacgagt
     3421 acatacgcca cgtttttgcg ggcatgcttc cagcgcaggc acgttgcttt tcgcaacttt
     3481 gcgagcacgt ggtttgcgta ccagctggtt aactgttgcc attaaatagc tcctggtttt
     3541 agcttttgct tcgtaaacac gtaataaaac gtcctcacac aatatgagga cgccgaattt
     3601 tagggcgatg ccgaaaaggt gtcaagaaat atacaacgat cccgccatca ccaggccatc
     3661 tggctggagt gcttaaccgt aagtctgacg aaatcagtat agtcaatggg aatgatgtcg
     3721 ttcgaaattc gaccagtcaa accgcgggca ataaggtctt cgttcagagc atagacctta
     3781 atgggggcat tacgcagact ttcaaggtag cggttgccgt caactgcggc agttacgcca
     3841 tcttgcaata atagcagttc gtcaccttca ctgagcagac gcagcagcgc agcaaagtcc
     3901 gtcagccagg gtgaacgatg taatgtgtgc agcataacgc ctcaaaacct caaaataaca
     3961 tcgtagttgg cgagttcgcg gcgtaaggca tctgcttcga gcggcgtggc ttcgaccaca
     4021 aagggtgtct gcggatctaa cccacgttcg cgcagtgaag ccgcgcaaat ccagcactgt
     4081 tcaatatcgt acagacccaa caatttaaaa gtggcaatgt aatcacgcgc cagcactgca
     4141 tcgggctttt gtcctggcag cagctgaaaa acgccatcag caataaagaa gacggccaga
     4201 tcgtcagtta atgcggaagt tgccagtaaa gcatctaaac cttcccggcc tgcagttgta
     4261 ccatgaggcg cagtagaaaa aacaaacgca attcgtttca tcagaactgt accaccctgt
     4321 cacaggtcag cgaggcttcc gccagcgcgc caagtccgct taaggtaaat cccggctgaa
     4381 ggtttgacga agcaagcccc agtcttccgg cctccgtttc atcaacaacg ccacggcgta
     4441 atgctgccgc tacgcagata ttcagcgcca caccatgttg cgcattcaac tgttgccagc
     4501 cgcgcacaag gtcaaattcg tcgcttgccg gtgaggtcaa ttggttagcg ttatagaccc
     4561 cttcccgata gaaaaaaacg ctgcttaact catggccttc tgctatcagc gcctgcgcaa
     4621 actgaaaagc actactcgcc tgttgcgtac cgtatgctgg cccggtcacc acgatggcaa
     4681 aacgcattac ttatcttgcc cctggaaatc accgctcttg aactggcgga tgtagagata
     4741 gacagtgtgt ttggagatgt tcaggcggtc agcaacctgg ttgatcgcgt ctttaatatc
     4801 gaagatccct ttctcgtaga gatttagcac gatctgacga tttttggcgt tattagaaac
     4861 attgcgatcg gcattcactt cttcgatggt gaactccagc gtttgggtaa ccagatcttc
     4921 aacagaagag gcaaagttga cgcttgaacc gacatccgga gtttccggcg gcacaaaagt
     4981 gctcataatc tgcgagaagg gaacatcaag attcatattg atgcacagca gaccaattac
     5041 acgctgttcg cggttacgaa tcgcgatagt cagggacttc attaatacgc cgcttttggc
     5101 gcgagtaaag tagcatttag aaacgctgct atccgctccc gtcatatcgt gcagcatacg
     5161 tagcgccagg tcagtaattg gcgaaccaat cttccggcct gtatgttcac cgttagcaat
     5221 gcgaatggct gaacatttta gatcctgcaa agagtgcaaa acgatttcac agtgggagcc
     5281 aataagcatc gctaacccgt ccaccaccgc ttcgtaggat ttcagaatat caaaatcggt
     5341 ctggtcaaaa ggacgttgat ccagtaaatc caactcactg gtttcgttgg ttaaaagcga
     5401 cctggacatg aaaaaaaaca ctccttttca ggagcctgtc gttaactttt cagggcaggc
     5461 tcattaatga tgcgggtaac taaattaata cagcggaggt tccgctttcc agcactaatt
     5521 atatccggcc tgtaataaaa aaaccgccgc ctggtcaggc ggcggttctt aatgcttatt
     5581 ttttagcaga gtctgcggct ttcgcatcag cttccggctt tgcatcagcc ttcggcgctg
     5641 gtttcacatc cagcagctct acgtcaaaca ccagggtaga attcggtggg atccccggaa
     5701 cacccgcttt gccgtaagcc agttctggtg gaataaccag tttgatctta ccgcctttct
     5761 tgatgttctt cagaccttct gtccaacccg gaataacacc gtccagacgg aaagagagcg
     5821 gttcaccacg ggtgtaagag ttgtcgaact ctttaccgtc gatcagcgta cctttgtagt
     5881 tcactacaac agtatcgctg tctttcggtg cttcgccttt accggcttct actacctgat
     5941 aaaccagacc agtggaagag gttttcacac ctttctcttt ggcaaatttc tcgcggtact
     6001 ctttaccttt tgcttcgtta tcagccgcgt ctttttccat cttcgcctga gcagaagact
     6061 tcacgcgagc ttcgaatgct tgcagagtct gttcgatctc ttggtcggaa agtttgctct
     6121 tatcagcaaa tgcatcctga acaccagcga tcagctgatc tttatccagt ttgatgccca
     6181 gtttttcttg ttctttaaga gagttttcca tgtaacgacc cagcgaagca cccagtgcat
     6241 aagctgattt ctgatcgtca tttttgaacg ctgctttgct gtcagcagtt gtagcaggtt
     6301 ttgcagcttc agcagcaaaa gtgattggtg catgcagggc aacggccatt gtggtcgcca
     6361 gcagcgttac tttaaacagt gatttcatcc atatctccag gatcggggca tctcacccca
     6421 gggttaacta ttatcagaag ggtactataa agcgttgtcg aacaaatcta catacagaca
     6481 cgccctatta tcatctattt tcagactctt tttgtttaaa ttagtttcga tgaccgcgaa
     6541 atgagtgctg tctcgggcag caaagttaag tagaatccgc ggcgaccatt cgacaaaaga
     6601 ggtgaatcat gcaggattta tcattggaag cacgcctggc agagctggag agccgactgg
     6661 cttttcagga aatcaccatt gaagaactga acgtcacggt gaccgctcat gaaatggaga
     6721 tggcgaaact gcgcgatcat ctgcgtctgc tgaccgagaa gttaaaagcc agccagccgt
     6781 cgaacatcgc gtcgcaggct gaagaaacgc ctccgccaca ttattgaggc gtaaaaaaag
     6841 cgggaattcc ccgctttttt gtcacctttt cggtattagt ggcaaccgca accgccgttg
     6901 cctttaccgc cacagcagcc ttcgccaccg tgttcatgac cgtgatcgtg gccatgacca
     6961 ccgcagcaac cgtcgtggtc gtgatcgtgg tggtgatcgt gcgcgccgtg aacgtgacca
     7021 tgagccagtt cttcttcggt cgcttcgcga atcgccacaa cttcaacgtt gaatttcagg
     7081 ttctgaccag ccagcatgtg gttaccatca accactacgt gatcgtcttc aaccgcagtg
     7141 atttcaaccg gtaccggacc ctggtcggtt tcagccagga aacgcatacc tacctgcagt
     7201 tcatcaacgc ccataaatac gtctttagga acgcgctgca ccaggttttc gtcgtactga
     7261 ccgtaagcgt cattcgcgcc aacagcgaca tcaaatttgt cgccaacttc atgaccttcc
     7321 agcgccgttt ccaggccaga gatcagggaa ccgtgaccat gcaggtagtc cagcggcgca
     7381 ctcaccggag actcatcaac caacacaccg tcttctgtac gtacctgata ggccaggctg
     7441 accaccaggt cttttgctac tttcatgata tctcctgagc atgggaagaa tagtggcgca
     7501 gattgtagcg gaattctgca gccgtgtact cactagctta aaaaaactcg gcgcatatcg
     7561 ctagtccgga tgaaaaatcc cgatcacttg ctcatctttg cgaacgtgat cgcgggcttc
     7621 tttgtctgct tctcgcatct gatgtccaca cttaacacat tcaacgatat caatattatt
     7681 ttcgcgccac atcgccattg aatcctgagc ctgacaggcc gggcattttg cgcccgcaat
     7741 aaaacgtttt cggattgcca tctttaccct ctactcaaat tcatcccagc cgtccagctg
     7801 gcgtcgttct tgttgcattt cacgctggaa aatctcttcc agttcgcgtc gggcttccct
     7861 ggcgcgagaa atttgtacgg tatcagcatg catcgggatt aactcccgca gcattcgcat
     7921 atccaggcgg cgaaaatgca gttgcgcgcg ctgcgcctga tgcggatgca tgccaagcgt
     7981 gaccagcgtc ttgcgcccca gctctaacgc actggagaat gtttcacggg aaaactgcgt
     8041 cacccctgcc tgtaataact catgcgcttc cacacgtccg cgcgctcgcg caagaatatg
     8101 caaatgcgga aagtgctgct ggcatatttc caccagcttc atagtgtctt ccggctcgtt
     8161 acaggtaatg acgatagact cagcggcctc tgcacccgca gaacgtaaaa gatctacctg
     8221 cgtggcgtcg ccgtaataaa ctttgtagcc atatttgcgc atcaggttaa cggcactgat
     8281 atcccgctcc agtacagtaa tgcgcatttt atttgccatc agcaaacgac caatcacctg
     8341 accaaaacgc ccaaagccca cgacaataac ctgcggttta tcatcgttga cccatggttt
     8401 ttcgtcttct tcttccggtc cgttaaactg gcgggatagc catttatcta ccagcttcat
     8461 cagcagcggc gtggtcatca tggaaagcgt caccgtcacc agcaacagcg ccatctggtc
     8521 gccctggaat aagcgttgtg aagaagcgga agaaaagagg acaaaggcaa actcaccgcc
     8581 ctgactcaac acgccagcaa actgcatccg ctctgagcta cgcacgccat acaatcgcgc
     8641 cagcagatac agcacgagaa ttttcaccgc caccagcaca accacactta tcactaccca
     8701 caacagatgg gtataaagca ccccgaggtt gagtgacatg ccgacagaga taaagaacaa
     8761 accgagcagc aagcctttga agggatcgat agccgtttcc agttcatggc gatattcact
     8821 ttccgccagt agcacacccg caataaacgt accgagcgcc atcgacaacc ccagcgcatc
     8881 cataaacaat gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca cttcccgcac
     8941 accagaagct gcaataaaac ggaataccgg acgcagtaaa tagcgcccgc caatcagcat
     9001 gccgacaaac gccagcacct tcatgccgat cttcatccag tcgaaatgtt cgtctgccga
     9061 acccgccaac aacggcacta acgccagtgc cgggatcacc gccagatcct gaaacagcag
     9121 taccgagaaa cctaactggc cggattcgct gcgattcatc cctttttcac gcatcaactg
     9181 caatgccatt gcggttgaag acatcgcaag gccaatgcca ccgaccaccg ccgcctgcca
     9241 ggcgaaatcc gtcagcatca ataatcccgc cagcaacgcc gcgcttaaca gcacctgtgc
     9301 cgcgcctacg ccaaaaatcg aacgccgcag ttgccaaagc ttggagggat tcaactcaag
     9361 gccgatgata aacatcagga ataccacgcc gagttccgaa aagtggagga tctcatcgac
     9421 gtcgctaata aaccccagcc cccacgggcc aatcgcaatc cccgccagca aatatcccaa
     9481 cactgcacca atacccagcc gcgatgccag cggcaccgca gccaccgccg cgaagagaaa
     9541 cagcactcct gcgagtaaaa aatcagaacc ttccatcagc ggcctcctgg agacaacgga
     9601 tttgccagcc agtcaccgta ggctctggcg tggctcgcca gctcctgtgc gctttgccgt
     9661 ctcgcccagt aaataatgat ggggcttaac caatgcatcc ggcacatgcc cgccgccagt
     9721 tcaaaggggc gcagaacatc gctcatcgga tagcgattca acgcgtcata acggtaagca
     9781 ctttccggct ccccggtggt aatcacgcta cgccagtact ttcccgccag ttggtttccc
     9841 cccggtccgc tggcaaaacc acggcttaat acccggtcca gccactcttt cagtagcgcc
     9901 gggcagctat aggtataaag aggatgctga aagacaatca cctcgtgctc gcgcagtaat
     9961 gcctgctcac gagggatatc aataaaaaag tcgggatagt gcgcgtaaag gtcgtgcacg
    10021 gtaacattgc tgagctgcgt ggccggttta agcagaaccc ggtttgccac cgagtcctga
    10081 gattccggat gggcatacag cagcaaaact ttcgctggct gagacatcat ccccctcccg
    10141 gtatggtttt tgtgtatagt cgctgttttg ggctaccatt gcgcccggtg cggcagctcg
    10201 cccatacatt acattatcat aatgataagt taacatagtc tgaacatacg gcgccttatg
    10261 attgtttttt cctcgttaca aattcgtcgc ggcgtgcgcg tcctgctgga taatgccacc
    10321 gccaccatca accctgggca gaaagtcggc ctggtgggta aaaacggctg tggtaaatct
    10381 accctgctgg cattgctgaa aaatgaaatc agcgccgacg gcggcagcta cacctttccg
    10441 ggaagctggc aactggcgtg ggtgaatcag gaaacaccgg cgttaccgca agcggcgctg
    10501 gaatatgtca ttgacggcga ccgtgaatat cgtcaactgg aagcgcagct acacgacgcc
    10561 aacgaacgta acgacgggca agccattgca accattcatg gcaagctgga tgctattgac
    10621 gcatggagta ttcgctcccg tgccgccagc ctgctgcacg gcctcggttt cagcaatgaa
    10681 caactggagc gcccggtaag tgatttctcc ggtggctggc gtatgcgtct taaccttgcc
    10741 caggcgctga tttgccgttc agacttgctg ctgctcgacg aaccgactaa ccacctcgat
    10801 ctcgatgccg ttatctggct ggaaaaatgg ctgaagagct atcagggcac gctgatcctg
    10861 atctctcacg accgcgactt cctcgatccg atcgttgata aaattattca tatcgaacaa
    10921 caaagcatgt tcgagtacac cggcaactac agttcgtttg aagtacagcg cgccacccgt
    10981 ctggcgcagc aacaagcgat gtacgaaagc cagcaggaac gcgtagcgca tctgcaaagt
    11041 tatatcgacc gtttccgtgc caaagccacc aaagcgaagc aggcccagag ccgtattaag
    11101 atgctggagc gtatggagct gattgccccg gcgcacgtcg acaacccgtt ccgctttagc
    11161 ttccgcgcgc cggaaagcct gcctaatccg ttattaaaga tggaaaaagt cagcgcaggc
    11221 tatggtgatc gcattattct cgactcgatt aaactgaatc tggtccccgg ctcgcgcatt
    11281 ggtctgctag gccgcaacgg cgcgggtaaa tcgacattaa tcaaactgtt agccggtgaa
    11341 cttgcgccag tcagcggtga aattggtctg gcgaaaggga tcaagctcgg ctacttcgcc
    11401 cagcatcaac ttgaatacct gcgcgccgac gaatcgccta ttcaacatct ggcacgttta
    11461 gcgccgcagg agctggaaca aaaactgcgt gattacctcg gcggctttgg tttccagggc
    11521 gataaagtaa ccgaagaaac gcgccgcttt tccggtgggg aaaaagcccg cctggtgctg
    11581 gcattaattg tctggcagcg tccgaatctg ctgctgctcg acgaaccgac taaccacctt
    11641 gacctcgaca tgcgtcaggc actcactgaa gcattaatcg agttcgaagg cgcgctggtt
    11701 gtcgtctcgc acgaccgtca tttgctgcgt tccaccactg acgatctcta cctggttcac
    11761 gatcgtaaag tcgaaccgtt cgacggcgat ctggaagatt atcaacagtg gttgagcgac
    11821 gtacaaaagc aggaaaacca ggccgacgaa gcgccaaaag agaacgcgaa cagcgcccag
    11881 gcacgtaaag atcagaagcg tcgggaagcg gagctgcgtg cgcaaaccca gccactgcgt
    11941 aaagagattg cccgtctgga aaaagagatg gagaagctga acgcgcaact ggcgcaggcg
    12001 gaagagaaac tcggcgacag cgaactgtat gaccagagcc gtaaagcgga gttgaccgcc
    12061 tgcctgcaac agcaagccag cgccaaatcc ggcctggaag agtgcgaaat ggcgtggctg
    12121 gaagcccagg agcagcttga gcagatgctg ctggaaggcc aaagcaactg atggcgcaga
    12181 taacgacgac cgatgccaat gaatttagca gcagtgctga attcacccct atgcgcggct
    12241 ttagcaattg tcatctgcaa accatgctgc cgcgtctgtt tcgtcgcaag gtgaaattca
    12301 ccccgcactg gcatcgactg gagttgcccg acggcgattt tgtcgatctc gcatggagtg
    12361 aagatcctgc acaggcgcgg cataaaccgc gtttagtggt atttcacggg ctggaaggca
    12421 gtctcaatag cccttacgct cacggtctgg ttgaggcggc gcaaaagcgc ggctggctgg
    12481 gcgtggtgat gcattttcgc ggatgcagcg gtgaaccaaa ccgtatgcac cgcatttacc
    12541 attcgggcga aaccgaagac gccagttggt ttttacgctg gctgcaacgc gagtttggtc
    12601 atgcgccaac ggctgccgtc ggctattcgc tcggcggtaa tatgctggcc tgtttgctgg
    12661 caaaagaagg taatgatctc ccgattgatg cggcggtgat tgtctctgcg ccgtttatgc
    12721 tggaggcctg tagttaccat atggaaaagg gcttttcccg catttatcag cgttacttgc
    12781 tgaacctgtt aaaagccaat gccgcgcgca agctggcagc ctaccccgga acactgccga
    12841 ttaatctcgc gcagttaaaa tcggtacgtc gcatccgtga atttgacgat ctcatcaccg
    12901 ccagaattca cggctacgct gacgctatcg actattatcg tcagtgtagc gccatgccga
    12961 tgctcaatcg gatagccaaa ccgacactga ttattcacgc caaagacgat ccgtttatgg
    13021 atcatcaggt gatcccgaaa ccggaaagtc tccccccgca ggtggagtat cagctgactg
    13081 aacatggcgg tcatgttggc tttattggcg gtacattact tcatccgcaa atgtggctgg
    13141 agtcacgcat tcctgactgg ttaacaacgt atctggaggc gaaatcatgt tgattccgtg
    13201 gcaagacctc tcccccgaaa cgctggaaaa tttaattgaa agctttgtgt tacgtgaagg
    13261 caccgattat ggtgaacatg agcgtacact tgaacagaaa gtcgccgacg tcaaacgcca
    13321 gctacagtgc ggagaagcgg tgctggtatg gtcggagctg cacgaaacgg tcaatatcat
    13381 gccgcgcagt cagtttcgcg aataacctgc aaccgtcggc ggacgagata ataatgatag
    13441 ctaagtcatg gagttactat gtctgccaaa catccggtca ttgcggtaac aggatccagc
    13501 ggcgcgggga ccaccaccac cagcctcgcg tttcgtaaaa tattcgcgca gttaaatctg
    13561 catgcagccg aggtggaagg cgacagtttt caccgttata cccgcccgga aatggacatg
    13621 gcgatccgca aagcgcgcga cgccgggcgg catatcagct acttcggccc cgaagcgaac
    13681 gacttcggcc ttctggaaca aaccttcatt gaatacggtc agagtggcaa agggaaatct
    13741 cgcaaatatc tgcataccta cgatgaagcc gtaccgtgga atcaggtacc ggggacattc
    13801 accccctggc aatctttacc ggaacccact gatgtactgt tttatgaagg tttacacggc
    13861 ggcgtagtca cgccacagca taacgttgcg cagcatgtgg acttactggt cggcgtggtg
    13921 cctatcgtta accttgagtg gattcaaaaa ctgatccgcg acaccagcga gcgcgggcac
    13981 tcacgagaag cagtgatgga ctcggtagtg cgttcaatgg aagactatat caactacatc
    14041 acaccgcagt tttcccgcac ccatcttaac ttccagcgcg ttcccaccgt cgacacctca
    14101 aacccgttcg cggcaaaagg tatcccgtca ctggatgaaa gctttgtggt gatccatttt
    14161 cgtaatctgg aagggatcga tttcccctgg ctgctggcga tgttgcaagg ctcattcatt
    14221 tcccacatca atacgttagt ggtgcctggc ggcaaaatgg gtctggcaat ggaattaatt
    14281 atgctgccgc tggtgcaacg attgatggaa ggaaagaaaa tcgagtaacg ctgctattac
    14341 gccggataaa atactatccg gcttcacaac gggatagtta aatcacgcgg caaccacttc
    14401 atacgagtga gtaatattca ccgctttttc cagcatcagc gccactgagc aatatttctc
    14461 ggcagaaaga tcaaccgcac gcgcaaccgc tgcgtctttc agatcgcgac cggtgacgat
    14521 aaaatgtaga ttaatgtgcg taaacaggcg tggtgcctct tcgcggcgct cagaggtcaa
    14581 ttttacttca caatcgacca catcctgacg ccctttttgc aggatagaaa ccacatcgat
    14641 ggcactgcaa ccacccgccg ccatcaacac catttccatc gggcttggtg ctttatcgcc
    14701 tgagttgccg tccattaaaa tctggtgacc agaggcggat tcgcccagaa aagttaaccc
    14761 ttcgacccac ttcactcgcg cttgcatttt tgctactcca ctgcgtcaat tttcctgaca
    14821 gagtacgcgt actaaccaaa tcgcgcaacg gaaggcgacc tgggtcatgc tgaagcgaga
    14881 caccaggaga cacaaagcga aagctatgct aaaacagtca ggatgctaca gtaatatatt
    14941 gatgtactgc atgtatgcaa aggacgccac attaccgtgc agtacagttg atagcccctt
    15001 cccaggtagc gggaagcata tttcggcaat ccagagacag cggcgttatc tggctctgga
    15061 gaaagcttat aacagaggat aaccgcgcat ggtgcttggc aaaccgcaaa cagacccgac
    15121 tctcgaatgg ttcttgtctc attgccacat tcataagtac ccatccaaga gcacgcttat
    15181 tcaccagggt gaaaaagcgg aaacgctgta ctacatcgtt aaaggctctg tggcagtgct
    15241 gatcaaagac gaagagggta aagaaatgat cctctcctat ctgaatcagg gtgattttat
    15301 tggcgaactg ggcctgtttg aagagggcca ggaacgtagc gcatgggtac gtgcgaaaac
    15361 cgcctgtgaa gtggctgaaa tttcgtacaa aaaatttcgc caattgattc aggtaaaccc
    15421 ggacattctg atgcgtctgt ctgcacagat ggcgcgtcgt ctgcaagtca cttcagagaa
    15481 agtgggcaac ctggcgttcc tcgacgtgac gggccgcatt gcacagactc tgctgaatct
    15541 ggcaaaacaa ccggatgcta tgactcaccc ggacggtatg caaatcaaaa ttacccgtca
    15601 ggaaatcggt cagattgtcg gctgttctcg tgaaaccgtg ggacgcattc tgaagatgct
    15661 ggaagatcag aacctgatct ccgcacacgg taaaaccatc gtcgtttacg gcactcgtta
    15721 atcccgtcag agtggcgcgt tacctggtag cgcgccattt tgtttccccc gatgtggcgc
    15781 agactgattt atcaccccga tatcaactat gcacttcgac aaacgctggt gctatgtttg
    15841 cccgtggccg ttgggttaat gcttggcgaa ttacgattcg gcctgctctt ctccctcgtt
    15901 cctgcctgtt gcaatattgc gggccttgat acgcctcata aacgtttttt caaacgctta
    15961 atcattggtg cgtcgctgtt tgccacctgt agcttgctga cacaggtgct actggcaaaa
    16021 gatgttcccc tgcccttttt gctgaccgga ttaacgctgg tacttggcgt cactgctgag
    16081 ctggggccat tgcacgcaaa attgcttccc gcatcgctgc tcgccgccat ttttaccctc
    16141 agtctggcgg gatacatgcc ggtctgggaa ccgttgctca tctatgcgct gggcactttg
    16201 tggtacggat tgtttaactg gttttggttc tggatctggc gcgaacaacc gctacgcgag
    16261 tcactaagtc tgctgtaccg tgaactggca gattactgtg aagccaaata cagcctgctt
    16321 acccagcaca ccgaccctga aaaagcgctg ccgccgctgc tggtgcgcca gcaaaaagcg
    16381 gtcgatctaa ttacccagtg ctatcagcaa atgcatatgc tttccgcgca aaataatacc
    16441 gattacaagc ggatgctgcg tattttccag gaggcgctgg atttgcagga acatatttcg
    16501 gtcagtttgc atcagccgga agaggtgcaa aagctggtcg agcgtagcca tgcggaagaa
    16561 gttatccgct ggaatgcgca aaccgtcgcc gctcgcctgc gcgtgctggc tgatgacatt
    16621 ctttaccatc gcctgccaac gcgttttacg atggaaaagc aaattggcgc actggaaaaa
    16681 atcgcccgcc aacacccgga taatccggtt gggcaattct gctactggca tttcagccgc
    16741 attgctcgtg tgctgcgcac ccaaaaaccg ctctatgccc gtgacttact ggccgataaa
    16801 cagcggcgaa tgccattact tccggcgctg aaaagttatc tgtcactaaa gtctccagcg
    16861 ctacgcaatg ccggacgact cagtgtgatg ttaagcgttg ccagcctgat gggcaccgcg
    16921 ctgcatctgc cgaagtcgta ctggatcctg atgacagtat tgctggtgac gcaaaatggc
    16981 tatggcgcaa cccgtctgag gattgtaaat cgctccgtag gaaccgtggt cgggttaatc
    17041 attgcaggcg tggcgctaca ctttaaaatt cccgaaggtt acaccctgac cttgatgctg
    17101 attaccaccc tcgccagcta cctgatattg cgcaaaaact acggctgggc gacggtcggt
    17161 tttactatta ccgcagtgta taccctgcaa ctgttgtggc tgaacggtga gcagtacatc
    17221 cttccgcgtc ttatcgatac cattattggt tgtttaattg ctttcggcgg tactgtctgg
    17281 ctgtggccgc agtggcagag cgggttattg cgtaagaacg cccatgacgc tttagaagcc
    17341 tatcaggacg cgattcgctt gattctgagc gaggacccgc aacctactcc actggcctgg
    17401 cagcgaatgc gggtaaatca ggcgcataat actctgtata actcattgaa tcaggcgatg
    17461 caggaaccgg cgtttaacag ccattatctg gcagatatga aactgtgggt aacgcacagc
    17521 cagtttattg ttgagcatat taatgccatg accacgctgg cgcgggaaca ccgggcattg
    17581 ccaccggaac tggcacaaga gtatttacag tcttgtgaaa tcgccattca gcgttgtcag
    17641 cagcgactgg agtatgacga accgggtagt tctggcgatg ccaatatcat ggatgcgccg
    17701 gagatgcagc cgcacgaagg cgcggcaggt acgctggagc agcatttaca acgggttatt
    17761 ggccatctga acaccatgca caccatttcg tcgatggcat ggcggcagcg accgcatcac
    17821 gggatttggc tgagtcgcaa gttgcgggat tcgaaggcgt aatacaggct aaaccatcgg
    17881 atgaggtgtt cacgccgcat ccgacatctt ttgcctgatg cgtcttattt acgccccaac
    17941 caccttcgcc accgcgtggg cgaaacgatg catcccttca tcgatatccg catcttccac
    18001 caccagcgac ggcgcaaaac gcatcacatc aggcccggca ttcagcacca ttacgccttc
    18061 ctctgcaccc gcatacagga aatcacgcgc ccgacctttg tactgtggtt tcagctctgc
    18121 gccaatcaac agccccatac cgcgaatatc gctaaacaca tcgtactgct gatcgatctt
    18181 ctgcaaatgg tcaacaaaac gctggcgttt cgcctgaata ccttccagca cttcaggggt
    18241 attgatgata tcaaacgccg cgcccgctac tgcacaggcc agaggattac cgccgtaggt
    18301 ggaaccgtga gaaccaggat gaaacgcgga agcaatttcc gccgtggtca gcatggcgct
    18361 aatcgggaag ccgccgccta acgctttcgc agaggtcaga atatccggcg tcacgccgta
    18421 gtgcatgtaa gcaaacaaat cgccggtccg ccccatcccg cactgtactt catcaaacac
    18481 caataatgcc tgatgttgat cgcacagctc gcgcaagccc tgcaaaaact ctggcgtcgc
    18541 tgccgtcaca ccgccctcac cctggatcgg ctcaaccacc accgcacagg tgtgatcatc
    18601 catcaccgct ttcactgcgt ggagatcgtt aaagggaacg tggatgatgt ctgccggttt
    18661 tggcccaaag ccatcggaat attttggttg cccgcccacc gagacggtaa acagcgagcg
    18721 accatgaaaa gcgttatgga aggcgataat tttggttttg aacgggctat gacgcacaca
    18781 ggcgtaatgg cgtgccagtt taaaggcggt ttcgttagct tccgtgccgg agttcataaa
    18841 caccacgcgt tcggcaaacg ttgcctcaat cagtttacga ccaagacgca gcgccggttc
    18901 attggtgaaa acgttactga tatgccacag agtttcgccc tgggttttta acgcgttcac
    18961 cagcgcagga tggcaatggc ccaacgccgt aactgcaatg ccacccgcga aatcgacata
    19021 ctccttgcct tgctgatccc agattcggct gccctgacct tttaccggaa taaactctgc
    19081 cggagcataa atcggcagga tcacttcatc gaaagtcgcg cgtgtaattg ctgtttgttc
    19141 aattgccatc tcatgatcac cctgttacgc ataaaccaat gtgaaattat aatcacaaaa
    19201 tatgcataaa aaatcactta atagcaatca gaaatcagcg atgcaggaaa ttagccagca
    19261 gttgatgtcc ttgttcgcta agaatacttt ctggatggaa ttgcacacct tccagatccc
    19321 actggcgatg gcgaatcccc ataatctcgc gggtttcgct ccaggccgtc acttcaaagc
    19381 actccggtaa tgagtcaggt tccaccacca gcgaatggta gcgtgtcacg gtgagtggat
    19441 ttgccagccc cttaaatacg cccacaccat tatgtgtaat cggcgaggtt ttgccgtgca
    19501 tgacctttgc ggcgcgcaca actttgccgc caaatgcctg tgccattgcc tggtgaccga
    19561 ggcagacgcc aagaatcggc aaacgcccgg catagtgacg aataacgtca agggagatcc
    19621 cggcttcatc tggcgtacag gggccgggtg agatgacaat tttttgtggt ttaagggcgt
    19681 cgatatccgc caacgtcaac gcatcgttgc gcttaaccaa cacatccgcc cccagttcac
    19741 aaaagtactg gtagaggttc caggtaaaag aatcgtagtt atcaataagc aggatcatgg
    19801 cggctccggt acgaaagaac cgcgctattt tactcagatt ccccgacttc gcttaccact
    19861 ttgctaaata tcatctgcag cgcggtgaga tcccccattg ccccattctg atttgcctga
    19921 ttccaggcct ctttttcgat accctgccag ctcagttgat atccggcatg aatcgccagt
    19981 tgctcgaaga agatccgctg tgccaaacca cttcccaccc ggaagggatg cagcacgttg
    20041 atttcacaat agtaatgcgc caaccgctcg acgaatttat ctttctcaag gctagtcaga
    20101 tacttctctt cctccaaatc ctgcatcagg gcattgccct ctttttcgag ataggcaaag
    20161 tggcagaacg gcgtatctcc ctgataaata tcaacttcac gcagttgccc tgcccagtcg
    20221 aaaatatcct gatacagctg gcgatggata gtgcgcaaat gcggtaaacc gcgcaccagc
    20281 ggaccaagct caatggtcgc agcacgcagc gccgtcattt cgtaagcggc ctgttccagc
    20341 cgctgctgct ggcggatgtt gagcttgtta cgcatgatat cgagacccgg gtagagatat
    20401 ggatcgcgcc cttcgccgaa tttatcgctc atagtgcctc ctcagcgctt caaggcgcgc
    20461 taaagcctct gcggcagtaa gagtgactaa aggcatctcg acgccttcaa gacggcgact
    20521 ggcctggaaa ttacgattac gctgaagctc ccagagacgg gacttttgct tatcggtgag
    20581 tttcttcact tgatgcctcc ctgaacgtgt ccatttgcca caagtataag cggcaaatcc
    20641 gggttacgcc gggagagcag aagcgcgggc aagattgccc gcgcgagaaa tcattacggc
    20701 aggactttag cggaaaggat aactaccggt tttgacggca cattctggta cggaccaacg
    20761 tcatgagtcg gcacctggga aatcttatcg gcaacgtcca tgcctttcac cactttacca
    20821 aataccgcgt aaccgaaatc acgctgaccg tggtcaagga aggcgttatc ggcaacgttg
    20881 ataaagaact ggctggtggc gctgtctttg tcagcggtac gcgccatcgc gatggtgcca
    20941 cgcgtgttgc gcaggccgtt atcagcttca tttttgattg gcgggtttgg ttttttctgc
    21001 tgcatctgct cggtgaaacc gccgccctga atcataaagc caggaatgac gcggtgaaag
    21061 gtcgtgttgt tataaaaacc actgttcaca taatcgacaa agttttgcac agacactggc
    21121 gctttttgtt tatccagctc cagttcgatg ttaccagctg aggttgtcaa caatacgtgc
    21181 gggtcccctt tcgctgccat tgctgcggga gaaagagcag aaagagcgaa aacagcagcc
    21241 atcgccgcca gggtcgattt gaacatgaga tttccttaac aaacagagca gaaaaaaagc
    21301 aagtgaagag attctaaata gccttcaaga acaaggccat ccctttacct aattttacgt
    21361 atctgaaata tatgtaacct gaggataact ttttattccg tgatcaaaat cacctcttaa
    21421 aatgcaattt agcaaccgat tgcaataaaa catttaatca gatcacaaaa tcgcctaaaa
    21481 tcgcccgctc gcggcatagc ccgctatgcc attttatttt tgaacgcagg ccaatcatga
    21541 ctaacagcaa tcgcatcaag ctcacatgga ttagctttct ttcctacgca ctgaccggtg
    21601 cgttggttat tgtcaccggg atggtgatgg gaaatatcgc cgattatttc aatctgcctg
    21661 tttccagcat gagtaacacc ttcaccttcc tcaatgccgg cattttaatc tctatcttcc
    21721 tcaacgcctg gctgatggaa atcgtcccgt tgaaaacgca gttacgtttt ggctttctcc
    21781 tgatggtgct ggcggttgcc ggtttgatgt tcagccacag cctggcgctg ttctcggcgg
    21841 cgatgttcat tctcggggtg gtcagcggca tcaccatgtc gattggtaca ttcctggtaa
    21901 cacaaatgta tgaaggacgt cagcgcggtt cacgcctgtt atttaccgac tccttcttca
    21961 gtatggccgg gatgattttc ccaatgatcg ccgcatttct gctggcgcgt agcattgagt
    22021 ggtactgggt ttatgcctgc atcgggctgg tgtatgtcgc tatttttatt ctgaccttcg
    22081 gctgtgagtt cccggcgctg ggcaaacatg cgcccaaaac ggatgctccg gtagagaaag
    22141 aaaagtgggg gatcggcgta ctgtttctct ctgttgcggc gctgtgctac atcctcggtc
    22201 agttaggttt tatctcctgg gtgccggaat atgccaaagg cctgggcatg agcctgaacg
    22261 acgcaggcac gctggtaagt aacttctgga tgtcgtacat ggtcggcatg tgggcattca
    22321 gctttattct tcgcttcttt gatttgcaac gtatcctgac cgtactggct ggtctggctg
    22381 cgattctgat gtacgtcttt aacaccggaa caccggcaca tatggcgtgg tcaattctcg
    22441 ccctgggctt cttctccagc gcgatctata ccaccatcat caccctgggt tcacagcaga
    22501 ccaaagtacc gtcgccaaaa ctggttaact ttgtcctgac ctgcgggacc atcggtacta
    22561 tgttgacctt tgtggttacc ggcccgattg ttgaacatag tggtccgcag gcggcgctgc
    22621 tgacggcaaa cggtctgtac gctgtcgtct ttgtgatgtg cttcctgtta ggtttcgtca
    22681 gccgtcaccg tcagcataac accctgacct ctcattaatt gttcatgccg ggcggcacta
    22741 tcgtcgtccg gccttttcct ctcttaccct gctacgtgca tctatttcta taaacccgtt
    22801 caatttgtct gttttttgca caaacatgaa atatcagaca attccgtgac ttaagaaaat
    22861 ttatacaaat cagcaatata ccccttaagg agtatataaa ggtgaatttg atttacatca
    22921 ataagcgggg ttgctgaatc gttaaggtag gcggtaatag aaaagaaatc gaggcaaaaa
    22981 tgagcaaagt cagactcgca attatcggta acggtatggt cggccatcgc tttatcgaag
    23041 atcttcttga taaatctgat gcggccaact ttgatattac cgttttctgt gaagaaccgc
    23101 gcatcgctta tgaccgcgta cacctctcgt cttacttctc tcaccacacc gccgaagagc
    23161 tgtcgctggt gcgcgaaggc ttctacgaga aacacggcat caaagttctg gtcggcgaac
    23221 gcgctatcac catcaaccgt caggagaagg tgattcactc cagcgccgga cgtaccgttt
    23281 tttatgacaa gctgatcatg gcgaccggtt cctacccgtg gatcccgcca atcaaaggtt
    23341 ctgatactca ggactgcttt gtctatcgca ctattgaaga cctcaacgcc attgaatcct
    23401 gcgcccgtcg cagcaaacgc ggtgccgttg ttggtggcgg cctgttaggt ctggaagccg
    23461 ctggcgcgct gaaaaactta ggcattgaaa cccacgttat cgaatttgcc cctatgctga
    23521 tggcggaaca gcttgatcag atgggcggcg agcagctgcg tcgcaaaatc gaaagtatgg
    23581 gcgtgcgcgt tcacaccagc aaaaacactc ttgagattgt gcaggaaggt gttgaagcgc
    23641 gtaaaaccat gcgttttgcc gacggcagcg aactggaagt cgactttatc gtcttctcta
    23701 ccggtatccg tccgcgcgat aagctggcaa cccagtgtgg tctggacgtt gctccgcgtg
    23761 ggggtatcgt cataaatgat tcctgccaga cttccgatcc ggatatctac gccatcggtg
    23821 aatgcgcaag ctggaacaac cgtgtatttg gtctggtagc accaggctac aaaatggcgc
    23881 aggtcgccgt tgaccatatt cttggtagcg aaaacgcctt tgaaggtgca gaccttagcg
    23941 ccaagctgaa actgctgggc gtagacgtag gcggtattgg tgatgcgcac ggtcgcacgc
    24001 ctggcgcacg tagctacgtt tacctcgacg aaagcaaaga gatctacaaa cgcctgattg
    24061 tcagcgaaga caacaaaacc ctgctcggtg cggtactggt gggcgatacc agcgactacg
    24121 gcaacctgct gcaactggtg ctgaacgcta tcgaactgcc ggaaaacccg gattctctga
    24181 ttctgccggc gcactcgggt agcggtaagc cgtctatcgg tgttgataaa ctgccggaca
    24241 gcgcgcaaat ctgctcctgc ttcgacgtca ccaaaggcga cctgattgct gctatcaaca
    24301 aaggctgcca caccgttgcg gcgctgaaag ccgaaaccaa agccggtacg ggctgcggcg
    24361 gttgtattcc gctggtcact caggtactga acgcggaact ggcgaaacag ggtatcgaag
    24421 ttaacaacaa cctgtgtgaa cactttgctt attcgcgtca ggaactgttc catttgatcc
    24481 gcgttgaagg cattaaaacc ttcgaagaac tgctggcgaa acacggcaaa ggttacggtt
    24541 gtgaagtttg taaaccgacc gtcggttcgc tgctggcctc ctgctggaac gaatacattc
    24601 tgaagccgga acatactccg ctgcaggatt ctaacgacaa cttcctcgct aacatccaaa
    24661 aagacggcac ctactcggtg atcccgcgtt ctccgggtgg tgaaatcacc ccggaagggc
    24721 tgatggcagt ggctcgtatc gcgcgtgaat ttaatctcta caccaaaatc accggctccc
    24781 agcgcctggc gatgtttggc gcacagaaag acgatctgcc ggagatctgg cgtcagctga
    24841 ttgaagcagg tttcgaaacc ggtcatgcct atgcgaaagc actgcgtatg gcgaaaacct
    24901 gcgtgggtag cacctggtgc cgctatggcg ttggcgacag cgtcggcctc ggcgtggaac
    24961 tggaaaatcg ctacaaaggc atccgtacgc cgcacaaaat gaagttcggt gtctccggct
    25021 gtacccgtga atgttcagaa gctcagggta aagacgtggg tattatcgcc actgaaaaag
    25081 gctggaacct gtatgtttgc ggtaacggcg gcatgaaacc gcgtcatgcg gatctgctgg
    25141 cagcggatat cgatcgcgaa acgctgatta aatatcttga ccgcttcatg atgttctaca
    25201 tccgtactgc cgacaaactg acgcgtaccg cgccgtggtt agaaaacctc gaaggtggca
    25261 tcgattacct gaaagcggtg atcattgacg acaaactggg gctgaacgca catctggaag
    25321 aagagatggc gcgcctgcgt gaagcggtag tgtgtgagtg gactgaaacg gtcaataccc
    25381 cgtctgcgca gactcgcttc aaacacttca tcaacagcga caagcgtgac ccgaacgtgc
    25441 agatggtgcc agagcgcgaa cagcaccgtc cggcaacgcc gtatgaacgt atcccggtaa
    25501 ctctggtgga ggacaacgca tgagccagtg gaaagacatc tgcaaaatcg atgacatcct
    25561 gcctgaaacc ggcgtctgcg cgctgttagg tgacgaacaa gtagcaattt tccgcccgta
    25621 tcacagcgac caggtgtttg cgatcagcaa catcgatccg ttcttcgagt ccagcgtgct
    25681 gtcacgcggg ctgattgcgg aacaccaggg cgagctgtgg gtcgccagcc cgctgaaaaa
    25741 gcagcgtttt cgcttaagcg acggcttgtg catggaagac gaacagtttt ccgtcaagca
    25801 ttacgacgcg cgagtgaaag acggcgtggt gcagctgcgc ggttaatatt ttaacgggag
    25861 gcgcaatgcc tccccctttt tgcatggtcc tgtaataacc ttcggtatat tgcaggacat
    25921 tttttaaact ttttgtttta ttttttgttt ttatttttta aaggataatc aaatgtttac
    25981 agacactatt actaagtgtg cggctaacgc tgcgcgcatt gcacgcctgt cggcaaataa
    26041 cccgctcggc ttttgggtca gctccgccat ggcgggcgcg tatgtgggtc ttgggatcat
    26101 cctgattttc acgctcggta atttgctcga cccgtccgta cgtcctctgg tgatgggtgc
    26161 gacctttggt atcgccttaa cgctggtgat tatcgccggt tctgaactgt tcaccggaca
    26221 caccatgttc ctcacctttg gagtgaaagc gggcactatc agccacgggc aaatgtgggc
    26281 aatcctgccg caaacctggc tgggtaacct ggtcggttcc gtctttgttg ccatgctcta
    26341 tagctggggt ggcggtagcc tgctgccggt cgataccagc atcgttcact ccgtcgcgct
    26401 ggctaaaacc actgcaccgg caatggtact gttcttcaaa ggtgcattgt gtaactggct
    26461 ggtttgcctg gcaatctgga tggcgctgcg cactgaaggg gcggcgaaat ttatcgctat
    26521 ctggtggtgt ttgctggcat ttatcgcgtc cggctacgag cactccatcg ctaacatgac
    26581 gctgttcgcc ctctcctggt tcggcaacca cagcgaagcc tacacgctgg cgggtattgg
    26641 tcataacctg ctgtgggtga cgctgggtaa tactttatcg ggtgccgtat tcatgggatt
    26701 gggttattgg tatgctacgc cgaaagcgaa tcgtccggtt gcggacaaat ttaatcaaac
    26761 tgaaacggct gccggttaat tattaagggg tttttacgtg gatcatttgc ctatattttg
    26821 ccaattacgc gatcgcgact gtctgattgt cggcggtggt gatgtcgcgg aacgcaaagc
    26881 aaggttgctg ttagacgcag gcgctcgctt aacggtgaat gcattagcgt ttattccaca
    26941 gttcaccgca tgggcagatg caggcatgtt aaccctcgtc gaagggccat ttgatgaaag
    27001 ccttctcgac acctgctggc tggcgattgc agcgacggat gatgacgcgc ttaaccagcg
    27061 cgtcagcgaa gccgctgaag ctcgtcgcat cttctgtaac gtggtcgatg cgccgaaagc
    27121 cgccagcttt attatgccgt cgattattga ccgctcgccg ctcatggtag cggtctcctc
    27181 tggcggcacc tctccggttc tggcgcgcct gttgcgcgaa aaactcgaat cactgctgcc
    27241 gttacatctg ggccaggtag cgaaatacgc cgggcaatta cgcgggcgag tgaaacaaca
    27301 gttcgccacg atgggtgagc gtcgccgttt ctgggagaaa ttgttcgtta atgaccgcct
    27361 ggcgcagtcg ctggccaaca acgatcagaa agccattact gaaaccaccg aacagttaat
    27421 caatgaaccg ctcgatcatc gcggtgaagt ggtgctggtt ggtgcaggtc cgggcgatgc
    27481 cgggctgctg acactgaaag gtctgcaaca aattcagcag gcagatgtgg tggtctacga
    27541 ccgtctggtt tctgacgata ttatgaatct ggtacgccgc gatgctgatc gcgttttcgt
    27601 cggcaaacgc gcgggatacc actgcgtacc gcaggaagag attaaccaga tcctgctgcg
    27661 ggaagcgcaa aaaggcaaac gcgtggtgcg gctgaaaggc ggcgatccgt ttatttttgg
    27721 ccgtggtggc gaagagctgg aaacactgtg caacgcgggt attccattct cggtggttcc
    27781 gggtattacc gcagcctctg gttgctctgc ctattcgggt attccgctca cgcatcgcga
    27841 ttatgcccag agcgtacgct taattactgg acacttaaaa accggtggcg agctggactg
    27901 ggaaaacctg gcggcagaaa aacagacgct ggtgttttat atggggctga atcaggctgc
    27961 aactattcag caaaagctga ttgaatacgg tatgcctggt gaaatgccgg tagcaattgt
    28021 cgaaaacgga acggcagtca cgcagcgcgt gattgacggt acgctcacgc agctgggcga
    28081 actggcgcag caaatgaaca gtccatcgct aattattatt ggtcgggttg ttggcctgcg
    28141 cgataaattg aactggttct ctaaccatta atttaaccag ggccagagaa gtctggcctt
    28201 tttcgtttac aactctcttt atatattcag cagataaaaa tataacccct taataaatat
    28261 tgtctgctaa tgggttatat tcaccgttac agcaacttac aaaattaagc caatcgctc
//
LOCUS       DACADF010000053        28247 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.053, whole genome shotgun sequence.
ACCESSION   DACADF010000053 DACADF010000000
VERSION     DACADF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.053"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            381..1520
                     /locus_tag="ISB30_002167"
     CDS             381..1520
                     /locus_tag="ISB30_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAP1989797.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTLGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1526..1969
                     /gene="wzb"
                     /locus_tag="ISB30_002168"
     CDS             1526..1969
                     /gene="wzb"
                     /locus_tag="ISB30_002168"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAP1989798.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYALLERSAHQWAQALNAEQV"
     gene            1972..4134
                     /gene="wzc"
                     /locus_tag="ISB30_002169"
     CDS             1972..4134
                     /gene="wzc"
                     /locus_tag="ISB30_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAP1989799.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTIIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLTGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKVLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4263..5102
                     /gene="wcaA"
                     /locus_tag="ISB30_002170"
     CDS             4263..5102
                     /gene="wcaA"
                     /locus_tag="ISB30_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HAP1989800.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5105..5593
                     /gene="wcaB"
                     /locus_tag="ISB30_002171"
     CDS             5105..5593
                     /gene="wcaB"
                     /locus_tag="ISB30_002171"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAP1989801.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITIGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5590..6807
                     /gene="wcaC"
                     /locus_tag="ISB30_002172"
     CDS             5590..6807
                     /gene="wcaC"
                     /locus_tag="ISB30_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAP1989802.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSVLNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6782..8002
                     /gene="wcaD"
                     /locus_tag="ISB30_002173"
     CDS             6782..8002
                     /gene="wcaD"
                     /locus_tag="ISB30_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAP1989803.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNYRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNIHPK"
     gene            8083..8829
                     /gene="wcaE"
                     /locus_tag="ISB30_002174"
     CDS             8083..8829
                     /gene="wcaE"
                     /locus_tag="ISB30_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HAP1989804.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLHFVSEPDNGIYDAMNKGIEMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8845..9393
                     /gene="wcaF"
                     /locus_tag="ISB30_002175"
     CDS             8845..9393
                     /gene="wcaF"
                     /locus_tag="ISB30_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAP1989805.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGHPAVVIRKRVETE"
     gene            9420..10541
                     /gene="gmd"
                     /locus_tag="ISB30_002176"
     CDS             9420..10541
                     /gene="gmd"
                     /locus_tag="ISB30_002176"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAP1989806.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10544..11509
                     /gene="fcl"
                     /locus_tag="ISB30_002177"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10544..11509
                     /gene="fcl"
                     /locus_tag="ISB30_002177"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAP1989807.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWQENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11512..11991
                     /locus_tag="ISB30_002178"
     CDS             11512..11991
                     /locus_tag="ISB30_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAP1989808.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGSDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11988..13211
                     /gene="wcaI"
                     /locus_tag="ISB30_002179"
     CDS             11988..13211
                     /gene="wcaI"
                     /locus_tag="ISB30_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAP1989809.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAAMVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13214..14650
                     /gene="cpsB"
                     /locus_tag="ISB30_002180"
     CDS             13214..14650
                     /gene="cpsB"
                     /locus_tag="ISB30_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAP1989810.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14755..16125
                     /gene="cpsG"
                     /locus_tag="ISB30_002181"
     CDS             14755..16125
                     /gene="cpsG"
                     /locus_tag="ISB30_002181"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAP1989811.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16181..17575
                     /gene="wcaJ"
                     /locus_tag="ISB30_002182"
     CDS             16181..17575
                     /gene="wcaJ"
                     /locus_tag="ISB30_002182"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAP1989812.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17577..19055
                     /gene="wzxC"
                     /locus_tag="ISB30_002183"
     CDS             17577..19055
                     /gene="wzxC"
                     /locus_tag="ISB30_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAP1989813.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDLLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIIIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19524..20804
                     /gene="wcaK"
                     /locus_tag="ISB30_002184"
     CDS             19524..20804
                     /gene="wcaK"
                     /locus_tag="ISB30_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAP1989814.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDKQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKAVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20801..22021
                     /gene="wcaL"
                     /locus_tag="ISB30_002185"
     CDS             20801..22021
                     /gene="wcaL"
                     /locus_tag="ISB30_002185"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAP1989815.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATSLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDSDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            22032..23426
                     /gene="wcaM"
                     /locus_tag="ISB30_002186"
     CDS             22032..23426
                     /gene="wcaM"
                     /locus_tag="ISB30_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAP1989816.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPAQQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGKGRFVLQDGS
                     QVTGEGGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMHNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23601..24494
                     /gene="galF"
                     /locus_tag="ISB30_002187"
     CDS             23601..24494
                     /gene="galF"
                     /locus_tag="ISB30_002187"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAP1989817.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDNASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQAVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            24867..25952
                     /gene="rfbB"
                     /locus_tag="ISB30_002188"
     CDS             24867..25952
                     /gene="rfbB"
                     /locus_tag="ISB30_002188"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP1989818.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYFFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSGLDDEKKKNFRFHHISTDEVYGDLPHPDEVNSNETLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNSLEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVFTICDL
                     LDEIVPKEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            25952..26851
                     /gene="rfbD"
                     /locus_tag="ISB30_002189"
     CDS             25952..26851
                     /gene="rfbD"
                     /locus_tag="ISB30_002189"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP1989819.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPKFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEEDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            26909..27787
                     /gene="rfbA"
                     /locus_tag="ISB30_002190"
     CDS             26909..27787
                     /gene="rfbA"
                     /locus_tag="ISB30_002190"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP1989820.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDNNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            27792..>28247
                     /gene="rfbC"
                     /locus_tag="ISB30_002191"
     CDS             27792..>28247
                     /gene="rfbC"
                     /locus_tag="ISB30_002191"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP1989821.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQRIFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSDLAEVLYKTNQYYAPSHEKNIIWNDLL"
BASE COUNT         7027 a         6817 c         7637 g         6766 t
ORIGIN      
        1 accttgcctg ctattccgtt agctgtaaca cttcctcctg cattattgga gagccaatat
       61 tcaattacca taatgtcacc ttatattcgg aagttaattt ccgcaataac cctttctcat
      121 caacgactgt atgcagagag ggtgtgaaaa attaagctaa atcagtgtgt tggtagctaa
      181 aaagccaggg gcggtagcgt gtctggatgc ctgaaagacc agtctgaatt attctgtcaa
      241 tagcctgcgg atgaaatcaa ttttttttag gactgatgcc agttaaattt ttattcaatt
      301 aactgcaagg taaatgtgca tggcgacatt gctattaata gtgcacagga taattactct
      361 gccaaagtga taaataaaca atgatgaaat ccaaaatgaa attgatgcca ttattggtgt
      421 cagtaacctt gataagcggt tgcacagtac ttccgggcag caatatgtcg acgctgggga
      481 aagacgtcat caaacagcag gacgctgatt tcgatctcga caaaatggtg aatgtttatc
      541 cgctgacccc gcgcctgatt gatcaattac gcccacgccc gaatgtagcg cgccccaata
      601 tgacgctgga aagcgagatc gcgaattacc agtatcgcgt cgggccgggg gacgttctta
      661 atgtcaccgt ctgggatcac ccggaactca ccacgccagc cggtcagtac cgcagctcca
      721 gcgacaccgg caactgggta cagcctgacg gcaccatgtt ctacccgtat atcggcaaag
      781 ttcacgtggt ggggaaaacg ctcgctgaaa tccgcagtga tattaccggg cgcttagcga
      841 cgtacatcgc tgacccgcag gtggacgtta atatcgccgc cttccgctcg caaaaagcct
      901 atatctccgg tcaggtgaat aaatccggtc aacaggccat taccaacgtg ccactgacca
      961 ttctcgacgc catcaacgcc gcaggtggcc tgaccgacac cgctgactgg cgcaacgtgg
     1021 tgctaacaca caatggtcgt gaagagcgca tttctttgca ggcgctgatg caaaacggcg
     1081 acctcaatca gaaccgactg ctttaccccg gcgatattct ttacgtgcca cgtaatgatg
     1141 atctgaaagt gtttgtgatg ggtgaagtga agaaacagag caccctgaaa atggacttta
     1201 gcggcatgac cctgactgaa gccctgggca atgctgaagg catcgacatg accacctcca
     1261 acgccagcgg catctttgtc attcgtccgc tgaaaggcga gagcgggcgt aacggcaaga
     1321 ttgccaatat ctaccagctg gatatgtccg atgcgacgtc gctggtaatg gcgacagaat
     1381 tccgcctgca accttatgac gtggtgtatg tcaccaccgc cccggtatcc cgctggaacc
     1441 gtctgatcaa tcagttgctg ccaactatta gcggtgtccg ttacatgacg gatacagcca
     1501 gcgacattca taactggtaa tcgtcatgtt taacaacatc ttagttgtct gtgtcggcaa
     1561 tatttgccgt tccccgacgg cggaacgctt actgcaacgt tatcacccgg agctaaaagt
     1621 ggagtccgct ggactcggcg cgttggtcgg taagggcgct gatcctaccg ctatcagcgt
     1681 cgccgcagaa catcaactgt ccctggaagg tcactgtgca cggcaaatca gccgccgcct
     1741 gtgtcgcaac tacgacctga ttctgaccat ggaaaagcgc catatcgaac gcttatgcga
     1801 gatggcaccc gagatgcgcg gcaaagtgat gctgtttggt cactgggata acgaatgtga
     1861 aatccccgat ccatatcgca aaagccggga aacgtttgca gcggtgtacg cattacttga
     1921 acggtctgcc caccagtggg cgcaggcatt gaacgcagag caggtataag aatgacagaa
     1981 aaagtaaaac aacatgccgc tccggtaacg ggcagtgatg aaatcgatat tggtcgcctg
     2041 gttggcacca tcattgaagc acgctggtgg gtgattggca ttaccgccgt attcgccctc
     2101 tgtgccgtgg tttacacctt cttcgctacg ccgatataca gcgccgacgc actggtacaa
     2161 atcgaacaaa gcagcggcaa ttcgttagtg caggatatcg gatcggcatt agccaacaaa
     2221 ccgcctgcat cggacgccga gatccagttg attcgttcgc gtctggtgct tggtaaaacg
     2281 gtggacgatc tcgaccttga tattgcggtg agcaaaaaca cgttcccgat tttcggtgcg
     2341 ggctgggagc gcctgacggg acgccagaac gagacggtga aagtgaccac ctttaaccga
     2401 ccgaaggaga tggcggatca ggtgtttacg cttaatgtgc tggacaacaa aaactacacc
     2461 ctgagcagcg atggcggctt tagcgcccgt gggcaagcgg gccagatgct gaaaaaagaa
     2521 ggcgtcacgc tgatggttga agccattcac gccagcccgg gcagtgagtt taccgtcacc
     2581 aaatactcca cgctggggat gatcaaccaa ctgcaaaaca gcctgacggt aacggagaac
     2641 ggcaaagacg caggtgttct gagcctgact tacaccggtg aagatcgcga acagatccgc
     2701 gacattctta acagcatcgc ccgtaactat caggaacaaa atattgagcg caaatcggcg
     2761 gaagcgtcga aaagcctcgc tttcctcgcg caacagttac cggaagtacg tagccgcctt
     2821 gatgttgccg aaaacaaact gaatgccttc cgtcaggata aagattcagt tgatctgccg
     2881 ctggaagcga aagcggtgct cgattcgatg gtgaacatcg acgcgcagtt gaacgaactg
     2941 acctttaaag aggcggaaat ctccaagctg tacaccaaag ttcacccggc gtaccgcacg
     3001 ctgctggaga aacggcaggc gctggaagac gaaaaagcca aacttaatgg tcgcgtaacg
     3061 gcgatgccga aaacccagca ggaaattgtg cgtctgaccc gcgatgtcga gtctggtcag
     3121 caggtctata tgcaactgct gaataaagag caggagctga aaatcaccga agccagcacc
     3181 gtcggcgatg tgcgcattgt cgacccggca atcacccagc caggtgtact gaaaccgaag
     3241 aaagtgctga ttatccttgg ggcgattatc cttggcctga tgctctctat cgtgggtgtg
     3301 ctgctgcgct cgttgtttaa tcgcggtatc gaaagcccgc aggtgctgga agaacacggt
     3361 atcagcgttt atgccagcat cccgctgtcg gagtggcaga aagcgcgcga tagcgtcaaa
     3421 accatcaaag ggattaaacg ctataaacag agccagctac tggcggtggg gaatccaacc
     3481 gatctggcga ttgaagccat ccgtagtctg cgtaccagtc tgcacttcgc gatgatgcag
     3541 gcacagaaca atgtgttgat gatgaccggg gttagcccgt caatcggtaa aacctttgtc
     3601 tgcgccaacc tggcggcggt gattagccag accaataaac gggtgttgtt gatcgactgc
     3661 gatatgcgca aaggctacac ccacgagctg ttgggcacta ataacgttaa tggcctgtcg
     3721 gaaattctga ttggacaggg cgatattact accgctgcta aaccgacctc tattgccaaa
     3781 tttgacctga tcccgcgcgg tcaggtaccg ccaaatcctt ctgaactgtt aatgagcgaa
     3841 cgctttgccg aactggtgaa gtgggcgagt aaaaactacg acctggtgtt gattgatacg
     3901 ccgccgattc tggcagtgac cgatgcggca attgttggtc gtcatgtcgg aaccacgttg
     3961 atggtggcgc gttatgcggt caacacattg aaagaagtgg aaaccagtct gagccgcttt
     4021 gagcaaaacg gtattccggt gaaaggggtg attctgaact ccatcttccg ccgcgccagc
     4081 gcgtatcagg attatggcta ttacgaatac gaatataagt cggatgcgaa ataacgagat
     4141 ccgctgtcgg atgcgatgct gacgcatctt atccgaccta cgttcacgcc gcatccggca
     4201 acagaacctg taaaccagct tataaaccgg cagaacatct cgatactccg ggagaacaac
     4261 ctatgaaaga caatccgctg atttcaatct atatgccgac ctggaaccgc caacaactgg
     4321 cgattcgggc gataaaatcg gtcctgcgcc aggactacaa caactgggag atgatcatcg
     4381 tggatgattg ctccacctcc tgggaacaac tacaacagta cgtcaccgcc ctcaacgatc
     4441 cgcgtatcac ttacattcac aacgacatta acagcggggc gtgcgcggta cgtaaccagg
     4501 cgattatgct ggcgcaaggg gaatacatca ccgggatcga tgacgatgat gaatggacac
     4561 ccaaccgcct gagcgtcttc ctcgcccata aacagcaact ggtcacacac gcctttttgt
     4621 acgctaacga ctacgtttgc cagggcgaag tttattccca accggcaagc ttgccgctgt
     4681 atcccaaatc gccgtattca cgccgcctgt tttacaaacg caatatcatt ggcaatcagg
     4741 tctttacctg ggcgtggcgt ttcaaagagt gcttgttcga taccgaactg aaagcggcac
     4801 aggattacga catcttcctg cggatggtgg tggagtacgg cgaaccgtgg aaagtggaag
     4861 aggcgacgca gatcctgcat atcaatcacg gcgaaatgca gatcacctcg tcaccgaaaa
     4921 aattctccgg atacttccac ttttaccgca agcacaaaga caaattcgac cgcgccagca
     4981 aaaaatatca gctgtttacc ctctaccaga tccgcaataa acgcatgacc tggcgcactt
     5041 tgctgacgct gctgtcggtg cgtaacggca aacgtctggc tgatggtatt cgggggcgct
     5101 aaagatgctg gaagatctgc gcgccaacag ctggagttta cgcccgtgct gcatggttct
     5161 tgcctatcgt gccgctcatt tttgttcggt gtggcgcaaa aagaacgtcc tcaacaatct
     5221 ttgggcggcc ccgctgctgg tgctgtatcg cattatcacc gaatgctttt tcggttatga
     5281 aatccaggcc gccgcgacca taggccgacg ctttactatc catcacggtt acgccgtagt
     5341 gatcaataaa aacgtggtcg cgggggatga tttcaccatt cgtcacggcg tcactatcgg
     5401 caatcgtggc gccgataaca tggcatgtcc acacattggc aacggcgtcg aactcggtgc
     5461 caacgtcatt attcttggtg atatcacgat tggtaacaac gtcaccgtgg gcgcgggcag
     5521 cgtggtgctc gactctgtcc cggacaacgc gctggtggtg ggagaaaaag cgcgagtgaa
     5581 ggtaattaaa tgaatattct gcaatttaat gtgcgactgg cggaaggcgg ggcagcaggt
     5641 gtggcattag atctccacca gcgcgcgctg caacaggggc tggcgtcaca ttttgtctac
     5701 ggctacggca aaggcggcaa agagagcgtc agccatcaga actatccgca ggtcatcaaa
     5761 catacgccac ggatgaccgc gatggcgaac attgcgttgt ttcgtctgct taatcgcgat
     5821 ctgttcggca atttcaatga gttatatcgc accattactc gcacaccggg cccggtggtt
     5881 ctgcattttc atgtgctgca cagctactgg ttgaatctta agagcgtggt gcgcttttgc
     5941 gaaaaagtga aaaaccataa accggatgtc actctggtct ggacgctaca cgaccactgg
     6001 agcgttaccg gacgctgcgc ctttaccgac ggttgcgaag gctggaaaac gggctgccag
     6061 aaatgcccga ccttaaataa ctatccaccg gtgaagattg atcgcgcgca ccagctggtg
     6121 gcgggtaaac gccagttatt ccgcgagatg ctggcactgg gctgtcagtt tatttccccc
     6181 agccagcatg tggctgatgc tttcaatagt ctgtacggtc cagggcgttg ccggattatc
     6241 aataatggca ttgatatggc aaccgaagcg attctggcgg acttgcctcc ggtacgcgaa
     6301 acccagggca agccgaaaat cgcggtggtg gcgcatgacc tgcgttacga cggcaaaact
     6361 aaccagcaac tggtacgcga gatgatggcg ctgggtgaca aaatcgaact gcataccttt
     6421 ggtaagttct cgccgttcac cgctggtaac gttgtcaatc acggctttga aactgacaag
     6481 cgcaagttaa tgagcgtgct caatcagatg gatgcgctgg tgttcagttc tcgcgtcgat
     6541 aactacccgc tgattttgtg tgaggcgtta tcgattggcg tgccggtgat cgccacccat
     6601 agcgatgcgg cgcgggaagt gctgcaaaaa tccggtggta aaaccgtcag cgaagaagat
     6661 gtgctgcaac tggtgcagtt aagcaaaccg gaaatcgcac aggcgatatt tggtaccacg
     6721 ctggctgaat tcagccagcg cagccgcgcc gcctacagtg gacaacagat gctggaggag
     6781 tatgtcaact tctatcagaa tctgtagcta cctgctgctg ccgctgatct acctgctggt
     6841 taacgtcaaa atcgcccagc ttggcgaaag tttccccatc accatcgtca cttttttacc
     6901 tgtcctgtta ctgctgtttt tagagcgcat cagcgttaaa aaattgatga ttgccttggg
     6961 gattggcgcg ggacttacgg cgtttaacta cctgtttggt cagtcgctgg atgccagtaa
     7021 atacgtcact tcaaccatgc tgtttgtcta tattgtgatc attattggca tggtgtggag
     7081 tattcgcttt aaaacgattt cgccacacaa ctatcgcaag atattacgtt tcttttatct
     7141 ggtggtcggg ctggtggtgg tgctggcggc ggtggagatg gcgcaaatta tcctcaccgg
     7201 cggtagcagt attatggagt cgatttcgaa atatctgatt tacagcaaca gctatgtgct
     7261 gaatttcatt aaattcggcg gcaagcgcac gacagcactt tatttcgaac cggcattttt
     7321 cgctctggca ttaatctcaa tttggctcag catcaaacag tttggtatca aaacgcctaa
     7381 aacagatgct atgattctcg cagggataat attatccggt tcattttcag gggttatgac
     7441 ctttatcctt ttttatttgc tggagtgggc atttcaatat ctgaataaag aggcgattaa
     7501 gaaaaagtta ccgttagcat tgatttctct ggctgtattc ctggttggtg tggtaatcgc
     7561 gttcccttat atttccaccc gtctgggcga tttaggtaca gaaggatcgt catcgtatta
     7621 tcgtattgtc ggtccgctgg tgatggtcgg ttattcttta acccatattg acggtgtagt
     7681 cagatttggc tcactttatg aatatgtcgc atcattcgga atatttaacg gtgcggatgt
     7741 cggaaaaacc atagacaatg gtttgtatct gctgattatt tatttttcct ggttcgcagt
     7801 gtttttatcg ctgtggtaca tgggaaaagt gataaaaatg atgatcaacg cttttggtga
     7861 taaccgcaat tttcgcgtgc aattatatct ttttactccg gtatcgctgt tttttaccgg
     7921 ttcgatattt agcccagaat atgcgttttt aatcgtctgc ccgtttattt tgcgaaaagc
     7981 gttaaatata caccctaaat aattcgagtt gcaggaagtg ggtgagcgag gaaaaccaac
     8041 gcacatgcag cttgaagtat gacgggtatt acgaggtaaa acatgttgct tagcataatc
     8101 actgtcgcgt ttcgtaacct cgaaggaata gtcaaaacac acgcctcgct ggcgcatctg
     8161 gcgcaggcgg acgatatcag cttcgaatgg attgttgttg atggcggttc caacgacggc
     8221 acccgtgagt atctggaaaa tctcaatggt atctataact tacactttgt cagcgaacca
     8281 gataacggta tctacgacgc catgaacaaa ggtattgaaa tggcgcaagg caagttcgcg
     8341 ttgtttctca actcgggcga tatttttcat caggatgccg catattttgt ccgtaagtta
     8401 aaagtgcaaa aagataacgt gatgatcacc ggcgatgcgc tgctggattt tggcgacggg
     8461 cataaaatta aacgtagcgc caaaccgggt tggtatattt accacagcct gcccgccagt
     8521 catcaggcga tatttttccc ggtgtccggc ttgaagaaat ggcgttatga cctggagtat
     8581 aaagtttctt ccgattacgc gctggcggcc aaaatgtata aagccggtta tgcatttaaa
     8641 aaactcaatg gcctggtgtc tgaattttct atgggtgggg tatctaccac caataatatg
     8701 gaattgtgtg ctgacgccaa aaaagtccaa cggcaaatat tgcacgtgcc tggcttttgg
     8761 gctgaattat cctggcattt acgccaacgt acgacctcaa agacgaaagc cttatataac
     8821 aaaagctgaa tataagggaa aacgatgcag gatttaagtg gattctcggt gccgaaaggg
     8881 ttccggggtg gcaacgctat taaagtgcaa ttgtggtggg cagtacaggc aacaatattt
     8941 gcctggtcgc cacaagtatt gtatcgctgg cgggcatttt tattacgttt attcggcgca
     9001 aaaataggaa aaaacgtagt cattcgtccg tcagtaaaaa ttacctatcc gtggaaatta
     9061 acgttaggtg attacgcgtg ggtcggcgat gaggtcaatt tatataccct cggtgaaata
     9121 accattggcg cacattcggt gatatcgcaa aaaagttatt tatgcactgg tagccacgac
     9181 catgcaagtc aacatttcac cattaacgcc acgcctattg tgattggcga gaaatgctgg
     9241 ctggcaaccg atgtctttgt tgcccctggc gtcacgatcg gcgacggcac cgtcgtgggt
     9301 gcgcgaagca gtgtttttaa atcgcttccg gcaaatgtgg tttgtcgggg gcaccccgca
     9361 gtggtgatac gcaaacgcgt tgaaactgaa taaattcaaa aaatacagag gaataagaca
     9421 tgtcaaaagt cgctctcatc accggtgtaa ccggacaaga cggttcttac ctggcagagt
     9481 ttctgctgga aaaaggttac gaggtgcatg gtattaagcg tcgtgcatcg tcattcaaca
     9541 ccgagcgcgt ggatcacatt tatcaggatc cgcacacctg caacccgaaa ttccatctgc
     9601 attatggcga cctgagtgat acctccaacc tgacgcgcat tttgcgtgaa gtgcagccgg
     9661 atgaagtgta caacctgggg gcaatgagcc acgttgcggt ttcttttgag tcaccggaat
     9721 ataccgcaga cgtcgatgcg atgggtacgc tgcgcctgct cgaggcgatc cgcttcctcg
     9781 gtctggaaaa gaaaacccgt ttctatcagg cttctacttc tgaactgtac ggtctggtgc
     9841 aggaaattcc gcagaaagag accacgccgt tctacccgcg atctccgtat gcggttgcca
     9901 aactgtacgc ctactggatc accgttaact accgcgaatc ctacggcatg tacgcctgta
     9961 acggtattct cttcaaccat gaatccccgc gccgtggcga gactttcgtt acccgcaaaa
    10021 tcacccgcgc aatcgccaac atcgcccagg ggctggagtc gtgcctgtat ctcggcaata
    10081 tggattccct gcgtgactgg ggccacgcca aagactacgt aaaaatgcag tggatgatgc
    10141 tgcaacagga acaaccggaa gatttcgtta ttgccaccgg cgttcagtac tccgtgcgtc
    10201 agttcgtgga aatggcggcg gcacaactgg gcatcaaact gcgctttgaa ggcacgggtg
    10261 ttgaagagaa gggcattgtg gtttccgtca ccgggcatga cgcgccgggc gttaaaccgg
    10321 gtgatgtgat tatcgccgtt gacccgcgct acttccgtcc ggcagaagtt gaaacgctgc
    10381 tcggtgaccc gaccaaagcg cacgaaaaac tgggctggaa accggaaatc accctcagag
    10441 agatggtgtc tgaaatggtg gctaatgacc ttgaagcggc gaaaaaacac tctctgctga
    10501 aatctcacgg ctacgacgtg gcgatcgcgc tggagtcata agcatgagta aacaacgcat
    10561 ttttattgct ggccatcgcg ggatggtcgg ttccgccatc aggcggcagc tcgaacagcg
    10621 cggtgatgtg gagctggtat tacgcacccg cgatgaactg aacctgctgg acagccgcgc
    10681 ggtgcatgat ttctttgcca gcgaaagcat tgaccaggtc tatctggcgg cggcgaaagt
    10741 gggtggtatt gttgccaaca acacgtatcc ggcagatttc atctaccaga acatgatgat
    10801 tgagagcaac atcattcacg ccgcgcatca gaacgacgtg aacaaactgc tgtttctcgg
    10861 atcgtcctgc atctacccga aactggcaaa acagccgatg gcagaaagcg agttgttgca
    10921 gggcacgctg gagccgacca acgagcctta cgccattgcc aaaatcgccg ggatcaaact
    10981 gtgtgaatct tacaatcgcc agtacggacg ggattaccgc tcagtcatgc caaccaacct
    11041 gtacgggcca cacgacaact tccacccgag taattcgcat gtgatcccag cattgctgcg
    11101 tcgcttccat gaggcgacgg cacagaatgc gccggacgtg gtggtgtggg gcagcggtac
    11161 gccgatgcgc gaatttctac acgttgatga tatggcagcg gcgagcattc atgtcatgga
    11221 gctggcacac gaagtctggc aggagaacac ccagccgatg ctgtcgcaca ttaacgtcgg
    11281 cacgggcgtt gactgcacta tccgtgagct ggcgcaaacc atcgccaaag tggtgggtta
    11341 caaaggtcgg gtggtttttg atgccagcaa accggatggc acgccgcgca aactgctgga
    11401 tgtgacgcgg ctgcatcagc ttggctggta tcacgaaatc tcactggaag cggggcttgc
    11461 cagcacttac cagtggttcc ttgagaatca agaccgcttt cgggggtaat gatgttttta
    11521 cgtcaggaag actttgccac ggtagtgcgc tccactccgc ttgtctctct cgactttatt
    11581 gtcgagaaca gtcgcggcga gtttctgctt ggcaaaagaa ccaaccgccc ggcgcagggt
    11641 tactggtttg tgccgggagg gcgcgtgcag aaagacgaaa cgctggaagc cgcatttgag
    11701 cggctgacga tggcggaact ggggttgcgt ctgccgataa cagcaggcca gttttacggt
    11761 gtctggcagc acttttatga cgataacttc tccggctcgg attttaccac tcactatgtg
    11821 gtgctcggtt ttcgcttcag agtagcggag gaagagctgt tactgccgga tgagcagcat
    11881 gacgattacc gctggctgac gccggacgcg ttgctcgcca gtgacaatgt tcacgctaac
    11941 agccgcgcct attttctcgc tgagaagcgt gccggagtac ccggattatg aaaatactgg
    12001 tctacggcat taactactcg ccggagttaa ccggcatcgg caaatacacc ggcgagatgg
    12061 tggaatggct ggcggcacaa ggtcatgagg tgcgggtcat taccgcgccg ccttactacc
    12121 cgcagtggca ggtgggcgag aactattccg cctggcggta caaacgggaa gagggggccg
    12181 ccatggtgtg gcgctgcccg ctgtacgtgc caaaacagcc gagcactctg aaacgcctgt
    12241 tgcatctcgg cagttttgcc gtcagcagtt tctttccact gatggcgcaa cgtcgctgga
    12301 agccggatcg cattatcggc gtagtgccaa cgctgttttg cacgccggga atgcgcctgc
    12361 tggcgaaact ctctggtgcg cgtaccgtgc tgcatattca ggattacgaa gtggacgcca
    12421 tgctggggct gggccttgcc ggaaaaggca aaggcggcaa agtggcacag ctggcgacgg
    12481 cgttcgaacg tagcggactg cataacgtcg ataacgtctc cacgatttcg cgttcgatga
    12541 tgaataaagc catcgaaaaa ggcgtggcgg cggaaaacgt cattttcttc ccaaactggt
    12601 cggaaatcgc ccgttttcag catgtcgcag acgccgatgt tgatgccctt cgtaaccagc
    12661 ttggcctgcc ggataacaaa aaaatcattc tttactccgg caatattggt gaaaagcagg
    12721 ggctggaaaa cgttattgaa gctgccgatc gcctgcgcga tgaaccgctt atttttgcca
    12781 ttgtcgggca gggcggcggc aaagcgcggc tggaaaaaat ggcgcagcag cgtggactgc
    12841 gcaacatgca attttttccg ctgcaatcgt atgacgcttt acccgcactg ctgaagatgg
    12901 gggattgcca tctggtggtg caaaaacgcg gcgcggcaga tgccgtattg ccgtcgaaac
    12961 tgaccaatat tctggcggta ggcggtaacg cggtgattac cgcagaagcc cacaccgaac
    13021 tggggcagct ttgcgaaacc tttccgggca ttgcggtttg cgtggaaccg gaatcggtcg
    13081 aggcgctggt ggcggggatt cgtcaggcgc tcctgctgcc caaacacaac acggtggcac
    13141 gtgaatatgc cgaacgcacg ctcgataaag agaacgtgtt acgtcaattt ataaatgata
    13201 ttcggggata attatggcgc agtcgaaact ctatccagtt gtgatggcag gtggctccgg
    13261 tagccgctta tggccgcttt cccgcgtact ttaccccaag cagtttttat gcctgaaagg
    13321 cgatctcacc atgctgcaaa ccaccatctg ccgcctgaac ggcgtggagt gcgaaagccc
    13381 ggtggtgatt tgcaatgagc agcaccgctt tattgtcgcg gaacagctgc gtcaactaaa
    13441 taaactcacc gagaacatta ttctcgaacc ggcggggcgt aacaccgcac ctgccattgc
    13501 gctggcggcg ctggcggcaa aacgtcatag cccggagaac gacccattaa tgctggtgct
    13561 ggcggcggat catgtgattg ccgatgaaga cgcgttccgt gccgccgtgc gtaatgccat
    13621 gccgtatgcc gaagcgggca agctggtgac cttcggcatt gtgccggatc tacctgaaac
    13681 cggatatggc tatattcgtc gcggtgaagt gtcggcgggt gagcaggata cggtggcctt
    13741 tgaagtggcg cagtttgtcg aaaaaccgaa tctggaaacc gcgcaggcct atgtggcaag
    13801 tggcgaatat tactggaaca gcggtatgtt cctgttccgt gccggacgtt atctcgaaga
    13861 actgaaaaag tatcgtccgg atattctcga tgcctgtgaa aaagcgatga gcgccgtcga
    13921 tccggatctc gattttattc gtgtggatga agaggcgttt ctcgcctgtc cggaagagtc
    13981 ggtagattac gcggtcatgg aacgcacggc agatgccgtt gtggtgccga tggatgcggg
    14041 ctggagtgat gtcggttcct ggtcctcttt atgggagatc agtgcccaca ccgccgaggg
    14101 caacgtttgc catggcgatg tgattaatca caaaactgaa aacagctatg tgtatgccga
    14161 atctggcctg gtcaccaccg tcggggtgaa agatttggtg gtagtgcaga ccaaagatgc
    14221 agtgctgatt gctgaccgta acgcggtgca ggatgtgaaa aaagtggtcg agcagatcaa
    14281 agctgatggt cgccatgagc atcgggtgca tcgcgaagtg tatcgtccgt ggggcaaata
    14341 tgactctatc gacgcgggcg accgctacca ggtgaaacgc atcaccgtga aaccgggcga
    14401 agggttgtcg gtacagatgc atcatcaccg cgcggaacac tgggtggttg tcgcgggaac
    14461 ggcaaaagtc actatcgacg gtgatatcaa actgcttggt gaaaacgagt ccatttatat
    14521 tccgctgggg gcgacgcatt gcctggaaaa cccggggaaa attccgctcg atttaattga
    14581 agtgcgctcc ggctcttatc tcgaagagga tgatgtggtg cgcttcgcgg atcgctacgg
    14641 acgggtgtaa acgtcgcatc aggcaatgaa tgcgaaaccg cgatgtaaat aacgacaaaa
    14701 acaaaattgg ccgtttcggt cagggccaac tattgcctga aaaagggtaa cgatatgaaa
    14761 aaattaacct gctttaaagc ctatgatatt cgcgggaaat taggcgaaga actgaatgaa
    14821 gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc tcaaaccgaa aaccattgtg
    14881 ttaggcggtg atgtccgcct caccagcgaa accttaaaac tggcgctggc gaaaggttta
    14941 caggatgcgg gcgtcgacgt gctggatatc ggcatgtccg gcaccgaaga aatctatttc
    15001 gccacgttcc atctcggcgt ggatggcggc attgaagtta ccgccagcca taacccgatg
    15061 gattacaacg gcatgaagct ggtgcgcgaa ggggctcgcc cgatcagcgg cgataccgga
    15121 ctgcgcgacg tccagcgtct ggcagaagcc aacgactttc ctcccgtcga tgaaaccaaa
    15181 cgcggtcgct atcagcaaat caatctgcgt gacgcttacg ttgatcacct gttcggttat
    15241 atcaacgtca aaaacctcac gccgctcaag ctggtgatta actccgggaa cggcgcggcg
    15301 ggtccggtgg tggacgccat tgaagcccgc tttaaagccc tcggcgcacc ggtggagtta
    15361 atcaaagtgc ataacacgcc ggacggcaat ttccccaacg gtattcctaa cccgctactg
    15421 ccggaatgtc gcgacgacac ccgcaatgcg gtcattaaac acggcgcgga tatgggcatt
    15481 gcttttgatg gcgattttga ccgctgtttc ctgtttgacg aaaaagggca gtttatcgag
    15541 ggttactaca ttgtcggttt gctggcagaa gcgttcctcg aaaaaaatcc cggcgcgaag
    15601 atcatccacg atccacgtct ctcctggaac accgttgatg tggtgactgc cgcaggcggc
    15661 accccggtaa tgtcgaaaac cggacacgcc tttattaaag aacgtatgcg caaggaagac
    15721 gccatctacg gtggcgaaat gagcgcccac cattacttcc gtgatttcgc ttactgcgac
    15781 agcggcatga tcccgtggct gctggtcgcc gaactggtgt gtctgaaagg aaaaacgctg
    15841 ggcgaactgg tgcgcgaccg gatggcggca ttcccggcaa gcggtgagat caacagcaaa
    15901 ctggcgcaac ccgttgaggc gattaaccgc gtggaacagc attttagccg tgaggcactg
    15961 gcggtggatc gtaccgatgg catcagcatg acctttgccg actggcgctt taatctgcgc
    16021 tcctccaaca ccgaaccggt ggtgcggttg aatgtggaat cacgcggtga tgtgccgctg
    16081 atggaagcgc gaacgcgaac tctgctgacg ttgctgaacg agtaatgtcg gatcttccct
    16141 tacccccact gcgggtaagg ggctaaaaac aggaacaacg atgacaaatc taaaaaagcg
    16201 cgagcgagcg aaaaccaatg catcgttaat ctctatggtg caacgctttt cagatatcac
    16261 catcatgttt gccgggctat ggctggtttg cgaagtgagc ggactgtcat tcctctacat
    16321 gcacctgttg gtggcgctga ttacgctggt ggtgttccag atgctgggcg gcatcaccga
    16381 tttttatcgc tcatggcgcg gtgttcgggc agcgacagaa tttgccctgt tgctacaaaa
    16441 ctggacgtta agcgtgattt tcagcgccgg actggtggcg ttcaacaatg atttcgacac
    16501 gcaattgaaa atctggctgg cgtggtatgg gctgaccagc atcggactgg tggtttgccg
    16561 ttcatgtatt cgtattgggg cgggctggct gcgtaatcat ggctataaca agcgcatggt
    16621 cgcggtggcg ggggatttag ccgccggaca aatgctgatg gagagcttcc gtaatcagcc
    16681 gtggttaggg tttgaagtgg tgggcgttta ccacgacccg aaaccgggtg gcgtttctaa
    16741 cgactgggcg ggtaatctgc aacagctggt cgaggatgcg aaagcgggca agattcataa
    16801 cgtctatatc gcgatgcaga tgtgcgacgg cgcgcgagtg aaaaaactgg tccatcaact
    16861 ggcggacacc acctgttcgg tgctgctgat ccccgatgtc tttaccttca acattctcca
    16921 ttcccgtatc gaagagatga acggcgtacc ggtggtgccg ctgtatgaca cgccgctttc
    16981 cggggttaac cgcctgataa aacgtgcgga agatattgtg ctggcgacgc ttatcctgct
    17041 gctgatctcc ccggtgctgt gctgcattgc gctggcggtg aaactgagct caccggggcc
    17101 ggttattttc cgccagactc gctacggcat ggatggcaag ccgatcaaag tgtggaagtt
    17161 ccgttccatg aaagtgatgg agaacgacaa agtggtgacc caggcgacgc agaacgatcc
    17221 gcgcgtcacc aaagtgggga actttctgcg ccgcacctcg ctggacgaat tgccgcagtt
    17281 tatcaatgtg ctgactggcg ggatgtcgat tgtcggtccg cgtccgcacg cggtggcgca
    17341 taacgaacag tatcgccagc tcattgaagg ctacatgctg cgccataaag tgaaaccggg
    17401 cattaccggc tgggcgcaga ttaacggctg gcgcggcgaa accgacacgc tggagaaaat
    17461 ggaaaaacgc gtcgagttcg accttgagta catccgcgaa tggagcgtct ggttcgatat
    17521 caaaatcgtt ttcctgacgg tattcaaggg cttcgttaac aaagcggcat attgatatga
    17581 gcttacgtga aaaaaccatc agcggcgcga agtggtcggc gattgccacg gtgatcatca
    17641 tcggcctcgg tctggtgcaa atgactgtgc tggcgcggat tatagataac caccagttcg
    17701 gtttgcttac cgtgtcgctg gtgatcatcg cgctggcaga tacgctttct gacttcggta
    17761 tcgctaactc cattattcag cgaaaagaaa tcagccacct tgaactcacc acgctgtact
    17821 ggctgaacgt cgggctgggg atcgtggtgt gcgtggcggt gtttttgttg agtgatctca
    17881 tcggcgactt gctgaataac ccggacctgg caccgttgat taaaacattg tcgctggcgt
    17941 ttgtggtgat cccccacggg caacagttcc gcgcgttgat gcaaaaggag ctggagttca
    18001 acaaaatcgg catgatcgaa accagcgcgg tgctggcggg ctttactttt acggtggtta
    18061 gcgcccattt ctggccgctg gcgatgaccg cgattctcgg ttatctggtc aatagtgcgg
    18121 tgagaacgct gctgtttggc tactttggcc gcaaaattta tcgccccggt ctgcatttct
    18181 cgctggcgtc ggtggcaccg aacttacgct ttggtgcctg gctgacggcg gacagcatca
    18241 tcaactatct caacaccaac ctttcaacgc tggtgctggc gcgtattctc ggcgcgggcg
    18301 tggctggggg atacaacctg gcgtacaacg tggccgttgt gccaccgatg aagctgaacc
    18361 cgatcatcac ccgcgtgttg tttccggcgt tcgccaaaat tcaggacgat acagaaaagc
    18421 tgcgcgtcaa cttctacaag ctgctgtcgg tggtggggat tatcaacttt ccggcgctgc
    18481 tggggctaat ggtggtgtcg aataactttg taccgctggt atttggcgaa aagtggagca
    18541 gcattattcc ggtgctgcaa ttgctgtgta ttgtggggct actgcgctcc gtgggtaacc
    18601 cgattggttc gctgctgatg gcgaaagcgc gggtcgatat cagctttaaa ttcaacgtat
    18661 tcaaaacctt tctgtttatt ccggcaatca tcattggtgg gcagatggcg ggcgcaatcg
    18721 gcgtcacgct tggcttcctg ctggtgcaga ttatcaacac cattctgagc tatttcgtga
    18781 tgattaaacc ggtgctcggt tccagttatc gccagtacat cctgagtttg tggctgccgt
    18841 tttatctctc gctgccgacg ctggtggtca gttatgcgct gggcattgtg ctgaaagggc
    18901 aactggcgct ggggatgctg ctggcggtgc aaatagccac gggcgtgctg gcgtttgtgg
    18961 tgatgattgt gctgtcgcgc catccgctgg tggtggaagt gaagcgtcag ttttgtcgca
    19021 gcgaaaaaat gaaaatgctt ttacgggcgg ggtgaatggt tgttttcggt tccggcaacg
    19081 gttgccggat gcggcgtaaa acgccttatc cgtcctgggg ggctgggctt ggatcttgta
    19141 ggtctgataa gacgcgttag cgtagcatca ggcactgaat gccggatgcg gcgtgaacgc
    19201 cttatccggc ctacggttct gtgctcggat cttgtaggcc tgataagacg cgtcagcgtc
    19261 gcatcaggca ctgaatgccg gatgcggcgt aaaacgcctt atccgtccta cggctctgtg
    19321 ctcgggtctt gtaggcctga taagacgcgt cagcgtcgca tcaggcactg aatgccggat
    19381 gcggcgtgaa cgccttatcc gtcctacggt tctgtgctcg gatcttgtag gcctgataag
    19441 acgcgtcagc gtcgcatcag gcaaaaaaca gcaatgaaaa caacaaacac agcctcacct
    19501 ggcatcaaat ttatgaggaa aaaatgaaat tacttattct gggcaaccac acttgcggca
    19561 atcgcggcga cagcgccatc ctgcgcggct tacttgatgc catcaatatc ctcaatccac
    19621 atgccgaagt ggacgtgatg agtcgctatc cggtcagttc ttcctggctg ctcaaccgcc
    19681 cggtaatggg cgatccgctg ttcctgcaaa tgaaacaaca caacagtgca gcgggcgttg
    19741 tcgggcgcgt taaaaaagtc ctccgtcgcc gttaccaaca tcaggtactg ctctcacgcg
    19801 tcaccgacac tggcaagttg cgtaatatcg ccatcgccca gggattcact gacttcgtgc
    19861 gcctgctgtc aggttacgac gccattattc aggtcggcgg atcgtttttt gtcgatctct
    19921 atggcgtgcc gcagtttgaa catgcgcttt gcacgtttat ggcgaaaaag ccgctgttta
    19981 tgattggtca cagcgtcggc ccgttccagg ataagcaatt taaccaactg gcgaactacg
    20041 tttttggtca ctgcgacgcg ctgatcctgc gcgaatcggt cagccttgat ttgatgaaac
    20101 gcagcaatat cactaccgca aaagttgaac atggcgtcga taccgcgtgg ctggtcgatc
    20161 accacacaga agacttcacc gccagctatg ctgttcaaca ctggctggac gttgccgcac
    20221 aacagaaagc ggtggccatt accctgcgtg aactggcacc gttcgacaaa cgcctcggca
    20281 ccactcaaca agcgtatgaa aaagcctttg ccggggtggt caatcgcatt ctcgacgaag
    20341 gctatcaggt gattgcgctc tccacctgta cgggcatcga cagctataac aaagacgacc
    20401 gcatggtggc gctcaatctg cgccagcaca tcagcgatcc tgcccgttac cacgtggtga
    20461 tggatgaact caacgatctg gaaatgggca aaattctggg ggcctgtgaa ctcaccgtcg
    20521 gtacgcgcct gcactccgcc attatctcaa tgaactttgc caccccggcg attgccatca
    20581 actatgaaca taaatccgcc gggatcatgc agcagctggg actaccggag atggcgattg
    20641 atatccgtca tttattagac ggcagcctgc aagcgatggt tgcggatacc ttaggccaac
    20701 ttccggcgct gaacgcacga cttaacgaag ccgtcagtcg cgaacgtcag acaggaatgc
    20761 agatggtgca gtctgtgctt gagcgcatcg gggaggtgaa atgaaggtcg gcttcttttt
    20821 attgaaattt ccgctgtcgt cggaaacctt cgtcctcaac caaatcactg cgtttattga
    20881 tatgggcttt gaggtggaga ttgtcgcgtt gcaaaaaggc gacacacaaa acacccacgc
    20941 ggcatggacg aaatacaacc ttgccgccag aacccgctgg ttacaggacg aacctacggg
    21001 caaagtggcg aagctgcgcc accgagccag ccagacctta cgcggcattc atcgtaaaaa
    21061 tacctggcag gcgctcaacc tcaaacgcta tggcgcagag tcgcggaacc tgattttgtc
    21121 tgccatttgt ggtcaggtcg caacatcgtt acatgccgat gtctttatcg cacattttgg
    21181 ccctgcgggg gtaaccgcag caaaactccg cgaactgggt gtcattcgcg gcaaaattgc
    21241 cactatcttc cacggcattg atatctccag tcgggaggtg ctcaaccatt acactcccga
    21301 atatcagcaa ctgtttcgcc gtggcgacct gatgctacca ataagcgatc tgtgggccgg
    21361 aaggctgcaa aaaatgggct gcccgaggga aaaaatcgcc gtatcgcgca tgggcgtgga
    21421 catgacgcgc tttagcccgc gtccggtgaa agcgcctgca acgccgctgg aaatcatttc
    21481 cgtcgcacgc ttaaccgaga aaaaaggcct gcatgtggcg attgaagcct gccgtcagtt
    21541 gaaagagcag ggcgtggcat ttcgctatcg catcctcggc attggcccgt gggaacgacg
    21601 cctgcgcacc ctcatcgaac aatatcaact ggaagatgtg gtggagatgc cgggctttaa
    21661 accgagccat gaagtgaaag agatgctcga tgacgcggat gtcttcctgt tgccatcggt
    21721 tacgggtgcg gatggcgata tggaaggtat tccggtggcg ctaatggaag cgatggcggt
    21781 cggtattccg gtggtttcta ctctgcatag cggaattcct gaactggtgg aggctgacaa
    21841 atccggctgg ctggtgcctg agaacgatgc tcgcgcactg gcgcaacgac tggcggcgtt
    21901 tagccaactg gacagcgacg aactggctcc tgtcgtcaaa cgtgctcgcg aaaaagtcga
    21961 acacgatttt aaccagcagg tgatcaatcg agaactcgcc agcttgctgc aggctttata
    22021 gaggagaacg catgccattt aaaaaactct cccgacgcac cttcctgacg gcaagttctg
    22081 tgcttgcctt cctccatacg cctttcgccc gcgcacttcc cgctcaacaa agcgttaaca
    22141 ttaacgacta caaccctcac gactggatcg cctcatttaa acaagccttc agcgaagggc
    22201 aaacggtcgt cgtgcccgcc ggattggttt gtgacaatat caacaccggc atcttcattc
    22261 ctcctggcaa aacgttacac atccttggaa gcctgcgcgg caacggcaaa gggcgatttg
    22321 tcttacagga cggcagccag gtgacagggg aggggggcgg cagtatgcat aacatcaccc
    22381 tggatgtgcg cggctctgac tgcaccatca aagggctggc gatgagcggc tttggtccgg
    22441 taacgcagat ttatatcggc ggcaaaaaca aacgggtcat gcacaacctg accatcgata
    22501 acctgaccgt tagccacgct aattacgcca tcttacgcca gggatttcat aaccagataa
    22561 tcggtgccaa catcaccaac tgtaagttca gcgacttaca gggcgacgct attgaatgga
    22621 acgtggcgat taacgacagt gatattttga tatccgacca tgtcatcgag cgcatcaact
    22681 gtactaatgg caaaatcaac tggggcattg gcataggcct tgcgggaagc acttatgata
    22741 ataattaccc agaagatcag gcagtgaaaa actttgtcgt ggcgaatatc acgggatcgg
    22801 attgtcggca actgatccat gttgaaaatg gtaaacattt tgttatccgt aatatcaaag
    22861 ctcgtaatat cacgccggat ttcagtaaga aagcaggtat tgataacgcc acagttgcta
    22921 tttatggttg tgacaacttt gtgattgata atattgaaat gattaatagt gccgggatgt
    22981 taatcggcta tggggtaatt aaaggcaaat atctctcgat accgcagaat ttccgagtga
    23041 atgatattca attggataat actcatcttg cttataaatt gcgcggcatc caaatctccg
    23101 ccgggaatgc tgtctccttt gtggcgctga ctaacattga gatgaagcgt gcgtcgctgg
    23161 agttacacaa caaaccgcaa catcttttta tgcgtaatat caatgtgatg caggaatcct
    23221 cagttggacc agcattgagc atgaactttg acatgcgcaa agacgttcga ggcgtgttta
    23281 tggcgaaaaa agaaacactg ctgtctcttg caaatgttca tgcggtgaat gaaaaagggc
    23341 aaagctccgt cgatatcgac aggattaatc accatattgt taatgtggaa aagattaact
    23401 ttagattgcc ggaacggaga gagtagattt gcgaccattc ctggaaaaat ggagtcatac
    23461 ttaggaacaa tgctactgca atccacaacg aagcggcgta acatcacaag taattcagta
    23521 atcaattcag ggtaattgat gctggcgaaa aaaatcgaac aagctataat tcagcaacca
    23581 ttttacaggt ggatgaaata atgacgaatt taaaagcagt tattcctgta gcgggtctag
    23641 ggatgcatat gttgcctgcc actaaggcga ttcccaaaga gatgctaccg atcgtcgaca
    23701 agccaatgat tcagtacatt gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc
    23761 tggtaactca tgcgtccaag aacgcggtcg aaaaccactt cgatacatct tatgaattag
    23821 aatctctcct tgaactgcgc gtgaagcgtc aactgctggc ggaagtacag tccatctgtc
    23881 cgccgggcgt gaccattatg aacgtgcgtc agggcgaacc tttaggttta ggccactcca
    23941 ttttgtgtgc gcgacctgcc attggtgaca acccatttgt cgtggtactg ccagacgttg
    24001 tgatcgacaa tgccagcgcc gacccgctac gttacaacct tgctgccatg attgcacgtt
    24061 tcaacgaaac gggccgcagc caggtgctgg caaaacgtat gccgggtgac ctctctgaat
    24121 actccgtcat tcagactaaa gagccgctgg atcgtgaagg taaagtcagc cgcattgttg
    24181 aatttatcga aaaaccggat cagccgcaga cgctggactc agacatcatg gccgtaggtc
    24241 gctatgtgct ttctgccgat atttggccgg aactggaacg tactcagcct ggtgcatggg
    24301 gacgtattca gctgactgat gccattgctg agttggcaaa aaaacaagca gttgacgcaa
    24361 tgctgatgac tggggacagt tacgactgcg gaaagaaaat gggttatatg caagcgtttg
    24421 tgaagtatgg gctgcgtaac ctgaaggaag gggcgaagtt ccgtaaaggt attgagaagc
    24481 tgttaagcga gtaacaaatt cctgactgga agtgtcggtc agtaagtaat taaaaacggc
    24541 agtgaagatt catagcataa gttttgctca ttgaatctca ctgccgttgt cttttttata
    24601 agtacaataa aaacagcatg ttagcttgca atcatagttc aaatttttcc aggattttcc
    24661 ttgtttctac agctgtttgg taagacaatt agcgtttgaa tttttcggat ttggcacgag
    24721 tgggttacgc tcgtcacatc gtggaaatgt acgcagtgct ctggtagcgg ttaagccagg
    24781 ggcggtagcg tgcattaatg catctattaa tcaaatctgg agcagtctat ttcacagcat
    24841 gctctctggc tatatggaat aaaaaagtga agatacttgt tactggtggc gcaggattta
    24901 ttggttctgc tgttgttcgt cacataataa ataatacgca agatagtgtt gttaatgtcg
    24961 ataaattaac atacgccgga aacctggaat cacttgcaga tgtttctgat tctgaacgct
    25021 atttctttga acatgcggat atttgtgatg cagctgcaat ggcacggatt tttgctcagc
    25081 atcagccgga tgcagtgatg cacctggcag ctgaaagcca tgttgaccgt tcaattacag
    25141 gccctgcggc atttattgaa accaatattg tgggtactta tgtcctttta gaagcggctc
    25201 ggaattattg gtctggtctg gatgatgaaa agaaaaaaaa cttccgtttt catcatattt
    25261 ctactgatga ggtgtatggt gacttacccc atccggatga agtaaatagc aatgaaacgt
    25321 tgccgctatt tacggaaacg acagcatacg cgccaagtag tccatattct gcttctaaag
    25381 cttccagcga tcatttggtt cgcgcatgga aacgtactta tggtttaccg accattgtga
    25441 ctaattgctc gaacaactat ggtccttatc atttcccgga aaagcttatt ccactggtta
    25501 ttcttaattc actggaaggt aaggcattac ctatttatgg caaaggagat cagatccgcg
    25561 actggttgta tgtagaggat catgctcgag cgttatatac cgtcgtaacc gaaggtaaag
    25621 cgggcgaaac ttataacatt ggtggacaca acgaaaagaa aaacatcgac gtagtgttca
    25681 ctatttgtga tttgttggat gagatagtcc cgaaagagaa atcttaccgc gagcaaatta
    25741 cttatgttac cgatcgtccg ggacacgatc gccgttatgc gattgatgct gagaagattg
    25801 gtcgcgaatt gggatggaaa ccacaggaaa cgtttgagag tgggattcgt aaaacggtgg
    25861 aatggtacct gtccaataca aaatgggttg ataatgtgaa aagtggtgcc tatcaatcgt
    25921 ggattgaaca gaactatgag ggccgccagt aatgaatatc ctcctttttg gcaaaacagg
    25981 gcaggtaggt tgggaactac agcgtgctct ggcacctctg ggtaatttga ttgctcttga
    26041 tgttcactcc actgattact gtggtgattt tagtaatcct gaaggtgtag ctgaaaccgt
    26101 aagaagcatt cggcctgata ttattgtcaa cgcagccgct cacaccgcag tagacaaagc
    26161 agaatcagaa ccgaagtttg cacaattact gaacgcgacg agtgtcgaag cgatcgcgaa
    26221 agcagccaat gaagtcggcg cctgggttat tcactactct actgactacg tatttccggg
    26281 gaccggtgaa ataccatggc aggaggagga tgcaaccgca ccgctaaatg tttacggtga
    26341 aaccaagtta gcgggagaaa aagcattaca agagcattgt gcgaagcacc ttattttccg
    26401 gaccagctgg gtctatgcag gtaaaggaaa taacttcgcc aaaacaatgt tgcgtctggc
    26461 aaaagagcgt gaagaattag ccgttattaa tgatcagttt ggtgcgccaa ctggcgcaga
    26521 gttactggct gattgtacgg cacatgctat tcgtgtggca ctgaataaac cggaagtcgc
    26581 aggcttgtac catctggtag ctagtggtac cacaacgtgg cacgattatg ctgcgctggt
    26641 ttttgaagag gcgcgcaaag caggcattcc ccttgcactc aacaagctca acgcagtacc
    26701 aacaacagcc tatcctacac cagctcgtcg tccacataac tctcgcctta atacagaaaa
    26761 atttcagcag aactttgcgc ttgtcttgcc tgactggcag gttggcgtga aacgaatgct
    26821 taacgaatta tttacgacta cagcaattta atagtttttg catcttgttc gtaatggtgg
    26881 agcaagatgt attaaaagga atgatgaaat gaaaacgcgt aaaggtatta ttttggcggg
    26941 tggttctggt actcgtcttt atcctgtgac gatggccgtc agtaaacagc tgttaccgat
    27001 ttatgataaa ccgatgatct attacccgct ctctacactg atgttagcgg gtattcgcga
    27061 tattctgatt atcagtacac cacaggatac tcctcgtttt caacaactgc tgggtgacgg
    27121 gagccagtgg ggcctgaatc ttcagtacaa agtgcaaccg agtccggatg gtcttgcgca
    27181 ggcgtttatt atcggtgaag agtttattgg tggtgatgat tgtgctttgg tacttggtga
    27241 taatatcttc tacggccacg acctgccgaa gttaatggac gtagctgtta acaaagaaag
    27301 tggtgcaacg gtatttgcct atcacgttaa tgatcctgaa cgttatggtg tcgtggagtt
    27361 tgataataac ggtactgcaa ttagcctgga agaaaaaccg ctggaaccaa aaagtaacta
    27421 tgcggttact gggctttatt tctatgacaa tgacgttgtg gaaatggcga aaaaccttaa
    27481 gccttctgcc cgaggtgaac tggaaattac cgatattaac cgtatttata tggaacaagg
    27541 acgtttgtct gtcgctatga tggggcgtgg ctatgcatgg ctggatacag ggacgcatca
    27601 aagtcttatt gaagcaagca acttcattgc caccattgaa gagcgccagg gactaaaggt
    27661 ttcctgtccg gaagaaattg cttatcgtaa agggtttatt gatgctgagc aggtaaaagt
    27721 attagccgaa ccgttgaaga aaaatgctta tggtcagtat ctgctcaaaa tgattaaagg
    27781 ttattaataa gatgaacgta attaaaactg aaattcctga tgtgctgatt tttgaaccaa
    27841 aagtttttgg ggatgaacgt ggcttctttt ttgagagttt taatcagagg atttttgaag
    27901 aagcagtagg tcgtaaggtt gagtttgttc aggataacca ttctaagtcc agtaaaggtg
    27961 ttttacgtgg tcttcattat cagttagaac cttatgctca aggaaaactg gtgcgctgtg
    28021 ttgttggcga ggtttttgat gttgcggttg atattcgtaa atcgtcacct acatttggga
    28081 aatgggttgg ggtgaatttg tctgctgaga ataagcgtca gttgtggatt cctgagggat
    28141 ttgcacatgg ttttttggtg ctgagtgatt tagcagaagt tttatataaa acgaatcaat
    28201 attatgctcc atcacatgaa aaaaatatta tatggaatga cctcttg
//
LOCUS       DACADF010000054        28161 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.054, whole genome shotgun sequence.
ACCESSION   DACADF010000054 DACADF010000000
VERSION     DACADF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.054"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            100..363
                     /gene="yhcN"
                     /locus_tag="ISB30_002192"
     CDS             100..363
                     /gene="yhcN"
                     /locus_tag="ISB30_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAP1989822.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(418..690)
                     /locus_tag="ISB30_002193"
     CDS             complement(418..690)
                     /locus_tag="ISB30_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989823.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(782..2749)
                     /gene="aaeB"
                     /locus_tag="ISB30_002194"
     CDS             complement(782..2749)
                     /gene="aaeB"
                     /locus_tag="ISB30_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAP1989824.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPCMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            complement(2755..3687)
                     /gene="aaeA"
                     /locus_tag="ISB30_002195"
     CDS             complement(2755..3687)
                     /gene="aaeA"
                     /locus_tag="ISB30_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAP1989825.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(3695..3898)
                     /locus_tag="ISB30_002196"
     CDS             complement(3695..3898)
                     /locus_tag="ISB30_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAP1989826.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            4081..5010
                     /gene="aaeR"
                     /locus_tag="ISB30_002197"
     CDS             4081..5010
                     /gene="aaeR"
                     /locus_tag="ISB30_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAP1989827.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(5132..6577)
                     /gene="tldD"
                     /locus_tag="ISB30_002198"
     CDS             complement(5132..6577)
                     /gene="tldD"
                     /locus_tag="ISB30_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAP1989828.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPVGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVNNLTVGGTA"
     gene            complement(6733..10533)
                     /locus_tag="ISB30_002199"
     CDS             complement(6733..10533)
                     /locus_tag="ISB30_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAP1989829.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTSITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLSVNVKVDGNLNSFELTGQAGADNYFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFARLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(10601..12070)
                     /gene="rng"
                     /locus_tag="ISB30_002200"
     CDS             complement(10601..12070)
                     /gene="rng"
                     /locus_tag="ISB30_002200"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAP1989830.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(12060..12653)
                     /gene="yhdE"
                     /locus_tag="ISB30_002201"
     CDS             complement(12060..12653)
                     /gene="yhdE"
                     /locus_tag="ISB30_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAP1989831.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(12662..13150)
                     /gene="mreD"
                     /locus_tag="ISB30_002202"
     CDS             complement(12662..13150)
                     /gene="mreD"
                     /locus_tag="ISB30_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP1989832.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(13150..14253)
                     /gene="mreC"
                     /locus_tag="ISB30_002203"
     CDS             complement(13150..14253)
                     /gene="mreC"
                     /locus_tag="ISB30_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP1989833.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(14319..15362)
                     /locus_tag="ISB30_002204"
     CDS             complement(14319..15362)
                     /locus_tag="ISB30_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAP1989834.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(15667..17607)
                     /gene="csrD"
                     /locus_tag="ISB30_002205"
     CDS             complement(15667..17607)
                     /gene="csrD"
                     /locus_tag="ISB30_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAP1989835.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLLSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     LVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17759..18733
                     /locus_tag="ISB30_002206"
     CDS             17759..18733
                     /locus_tag="ISB30_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP1989836.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVTDLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            19710..20180
                     /gene="accB"
                     /locus_tag="ISB30_002207"
                     /gene_synonym="fabE"
     CDS             19710..20180
                     /gene="accB"
                     /locus_tag="ISB30_002207"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP1989837.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            20191..21540
                     /gene="accC"
                     /locus_tag="ISB30_002208"
     CDS             20191..21540
                     /gene="accC"
                     /locus_tag="ISB30_002208"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP1989838.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRSDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(21632..22678)
                     /locus_tag="ISB30_002209"
     CDS             complement(21632..22678)
                     /locus_tag="ISB30_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP1989839.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGVVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDSIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVMRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSITPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(22675..23637)
                     /locus_tag="ISB30_002210"
     CDS             complement(22675..23637)
                     /locus_tag="ISB30_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1989840.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLMEIETFIQRHDMSIREAAQ"
     gene            complement(23659..24648)
                     /locus_tag="ISB30_002211"
     CDS             complement(23659..24648)
                     /locus_tag="ISB30_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1989841.1"
                     /translation="MTEKTLASPKPSGIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFVLLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(24649..26148)
                     /locus_tag="ISB30_002212"
     CDS             complement(24649..26148)
                     /locus_tag="ISB30_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP1989842.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKPDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(26209..27099)
                     /locus_tag="ISB30_002213"
     CDS             complement(26209..27099)
                     /locus_tag="ISB30_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP1989843.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLSGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            complement(27135..27989)
                     /locus_tag="ISB30_002214"
     CDS             complement(27135..27989)
                     /locus_tag="ISB30_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HAP1989844.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLRVDEKDSAYTSPAAA
                     TEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
BASE COUNT         7080 a         7547 c         6694 g         6840 t
ORIGIN      
        1 gcgtggtatt aattagttca ataattagtg tatacttgat tttgtgatat gggtcacgaa
       61 acaaaggccc agctaaaaga ttatgtcgag gtaaaaatca tgaaaatcaa aaccactgtt
      121 gctgcattaa gcgtactttc tgttctctct ttcggtgcat tcgctgccga ctccattgat
      181 gctgcacaag cacaaaatcg tgaagcaatc gggaccgtat ccgtaagtgg tgtggcgtct
      241 tcgccaatgg atatgcgtga aatgctgaac aaaaaagcgg aagagaaagg cgcaacggcc
      301 taccagatta ctgaagctcg tagtggtgac acctggcacg ctacggctga actgtacaaa
      361 taaaccctca tcgtcttgtc cgacgatatt gcccccggtt cgggggcttt ttttgcgcta
      421 atgacgaaca ttaaaacgca aatgcccttc cagctcttcc tctgcctcat caaacagcaa
      481 tattaacgcg ccaaaacggc gacgcgtttt ctctcccaga tgaacaaatt caatctcaag
      541 tggtagcggc aggacatcgt tcattaacac atcccatagt gagtcgagat cgcgtacctt
      601 atctttcgcc agaccaaagg tttggctaaa gtcacgataa aaatcctcct gactctcaat
      661 ctcatcaaaa tcaaaggtat aaatattcat ctgttgccac cgtcacgttt cgcggcgagt
      721 tctgccgttc atcgctaagt atagccatga aaaaaccgac gcttcaggcg tcggttttga
      781 cttaactatc ggtcaacgca tgttgatact tatggagcat acccgtaagc cgatgtactg
      841 gttccgtcac ctgcggtggc gcttgccaga tgcgtagctt ttcctggtag atttccagtt
      901 cttccagcaa ctggccaaag taccgacgac gtttatcatc gctacgggca gatatcacat
      961 ggtctgctgt gcggcgcatt tgtcggtgaa acgccgataa atcctcgtta accgggatcg
     1021 gcgcatcacg caggcgctgg tgcgcgataa tcatcgtcag cgccaggcga aatttcggca
     1081 aatcccctgg gaacttattc atcagcaaaa acagctgctg ataaagtgcc gggaggtgat
     1141 tctctttacg acgtgccaca ttggtagtca tcgcggaaac agcggcagaa acaaactgat
     1201 taagcagtac acgtccagtc ctgtcgcgcg atttatcccg caccagcaaa ataacggtga
     1261 acgcgagcac acagccgacg atttgcccta atgcgctgtc gagaaactga ctgaaatgga
     1321 aagtcatcgg gttatccagc acgataatgt ttatggtgct ggccaacgcc cccatcgagc
     1381 ccaatctccg tttctgtact tctatgccga ggaaaaatcc cagcactgcc aggctaatac
     1441 acagcagcaa catgctctgt tgggtattag ggataatcac caaaaagtag agcagcccta
     1501 acggcagcgc tgccagcgtc ccgtagataa agtcgatcgc caccatgcat ggattcggca
     1561 aacgcattgc cagtgacgtc acgaccgcaa tcatcaccat tgcaccactg ccggaagtcc
     1621 agcccgtcca cagccagaaa agcgtgccca gaatgcaaga aagtgtggtt cgccagaagt
     1681 taaccattgc atgatgacgt tcggctgact ctacttttac ttccggttcg ccttgcagga
     1741 tctcttcttc ggtggcgttg atttttgtgt tactgataac gccgcgcttg agaagctgat
     1801 aacgcgttgc cgccgcgacc caactataaa tggtaacagg cgtttcccgt tccccggtcc
     1861 aggcgatcac tctccgcagg cgtttgagct gcttgtggac gtcctgcgca gtttctaccg
     1921 gcgtgtcaaa aaattcgcgg aaagtatcag tgatcaattc cgggcgcgta ttctgaataa
     1981 gataagtttc gcaggattgg gtaatcagcg tcagcgatag cgtattgatc gcttttaaac
     2041 gtcgattagc ccgcgcccag cgggaagatt ccatattcag gttgctgcgc ataccttgta
     2101 acgccgtagt acgtcgcacc agatcgcccc aggctttatc gacaacttca ccatcgccat
     2161 gcttgataca gagttgcatt aattgatatt gcgcgaccag caaactttcc agctctcgat
     2221 ccacttcttg tttgatcgat cgcggagaaa agagcaaatc cgccataatt gcacacacaa
     2281 taccgataac gatctcgcta caacgttcga cggcaaactg cggcgtaagc aatggttccg
     2341 gctgaatggt gatcacaatg atcagcgcgg tataaccggc cagcccccac gcatacgagt
     2401 tttctattcg taccagcgag gatatccagg tacaaaaacc ggcccagata cagcacacca
     2461 gaatcatcaa taatggtgct cggatcatcg caatgatgat taccagtccg gcaatacagc
     2521 caataaatgt gccgatgatg cgcaaaaagc cacgatagcg aatagcgcca gaatacggtt
     2581 cacctcccgc agcaaaggcc ggaccggcgg caacaatcgc cgctgtcagt accgcccagc
     2641 gtggcgtttc cagctggaag tgaaagccaa caaacagcgc cagtacaatg gcggtcgcca
     2701 gttttaccgc aaagcgaata tgttgattag caatggagaa aatacccatc gtgattaacc
     2761 aaactcacgc aggcgatgag ccattttacg gaagaacgaa tcctgacttt cgtcgcgatc
     2821 ttgtttgcca gtgactacca ccgtagcagt ggtgcccgca ggccagatgt tctcttgctg
     2881 gttgtcgaga cgaatacgta ccggaacacg ttgcgccaaa cgtacccatt caaggttaga
     2941 gtcgatggtc gccatccctt tgtcgtcacg cgtgctgctg gcattggtga cccctgcggc
     3001 aacactatca acagtccctt tcagcacttt gttactgcca agcggcgtga tctccgcacg
     3061 atacccaggg cgcacccctt ccagcttagt ttcttccata taggccagta catagaagga
     3121 gttctgtttc accagcgcaa ccgccgttga tccacgggta ataaactcac cggtatagac
     3181 gttgaggttg gtcacccagc catctgctgg cgcgcggatc accgtgcgtt caagatccag
     3241 ttttgccaga tcgcgggtcg cctgcgcttt cgctaactga tgcagaaccg tttgtagtac
     3301 gttgttggcc tggtcgatct cttcgcggga catcgcctgc acaccgagac ggttacgacg
     3361 tccggcctcc tggcgtttct cctgtgccag tacctgataa taagcaacat cggcttgcgc
     3421 ttcctcaagc gccttttgat agcgcggctg gtcgatggtg aacagtacct gtcctttttt
     3481 caccagctgg ttatcatgaa cattcacctg ggtaatgagt ccagaaacgt ccggcgcgat
     3541 cgcaacgacg tcagcgctaa agcgcgcgtc acgcgtccac ggggattcgg tgtaatagac
     3601 ccaggcatta aaaattgcga tgaaggccag aatgactaat acgaccgtga tggccgtacg
     3661 ggagaatttt cttattagtg ttttcacttc aacctcaaac gaacagtcgc gatatcaaat
     3721 aaaacaagca gcaatagagc gcggtgttga acaacgccgg atgccagaca aagtcgtaga
     3781 tacctgttgg cacaagtacc cggcgcacca gccagaaaat cgccagtgat aaaagcaatt
     3841 cgaaaaatat cggtgggaag gacagcccaa acaccacgat aacgggaaac agactcatgt
     3901 tgaccttggt tgtatagaga gagcaggcgt tattattttc agcatctgtc gccgcagaga
     3961 agggcataga aagccgggcg agagcaacat tgctgtagat tgatatttaa tatattaacg
     4021 taactgttat gctgttatct atattatgtg atctaaatca cttttaagtc agagtgaata
     4081 atggaacgac taaaacgcat gtcggtgttt gccaaagtag ttgaatttgg ctcttttacc
     4141 gccgccgcca gacagctaca gatgagcgtt tcgtccatca gtcagacggt gtcaaaactg
     4201 gaagatgagt tgcaggtaaa gctgttaaac cgtagcacac gcagcattgg cctgaccgaa
     4261 gccggtagaa tttactacca gggctgccgt cggatgctcc atgaagtgca ggatgttcat
     4321 gagcaactgt atgccttcaa taacaccccc atcgggacgc tgcgcattgg ctgttcttca
     4381 actatggcac aaaatgttct cgccgggctg acagcaaaaa tgctgaaaga atacccaggt
     4441 ttgagcgtca atctggttac cggaattcct gcccccgacc tgattgccga cggtctggat
     4501 gtggtgatcc gcgtcggcgc gttgcaagat tccagcctgt tttcccgccg tctgggcgcg
     4561 atgccaatgg tggtgtgcgc cgcaaaaagc tatctcacac aatacggcat accggaaaaa
     4621 cccgccgatt taagtagtca ctcatggctt gaatacagcg tgcggcccga caatgaattt
     4681 gaactgatcg caccggaagg gatctcgact cgcctgatcc cacaaggaag atttgtgact
     4741 aatgatccga tgacgctggt gcgctggctg acggcgggtg ccgggatcgc ctacgtgccg
     4801 ctgatgtggg tgatcaacga gatcaatcgt ggggagctgg agatcctgct gccacgttac
     4861 cagtcagatc cacgcccggt ttatgcgtta tataccgaaa aagataagct gccgctgaag
     4921 gtacaggtcg tgatcaactc gctgacggat tattttgttg aggtcggtaa attgtttcag
     4981 gagatgcacg ggcgcgggaa agagaagtaa ttaatattgt actgttacgt tgtacaaacc
     5041 tgtgccgacg ggttccccta accccagagg ggcgagggga ccgttcgtgc acggagaagg
     5101 attaattatc cttctgaaaa tactgaaatt attacgcagt accgccaaca gtcaggttat
     5161 taactttcag cgttggctgg cccacgccaa ccggcaaact ttgcccttct ttaccgcaga
     5221 cacccacgcc gttatccagt ttcaggtcgt tgccaaccat cgaaatctgc tgcatggttt
     5281 cgataccgga accaatcaac gttgcgcctt tcaccggctt agttacttta ccgttttcaa
     5341 tcagatacgc ttctgaggtg gagaaaacga atttgccgga ggtgatatcc acctgaccgc
     5401 caccaaagtt cggtgcatag ataccgtact caacggattc aataatttcc tgcggggtcg
     5461 atttacccgg cagcatatag gtgttggtca tacgcggcat gggcagatgg gcgtaggatt
     5521 cacggcgacc gttgccagtc ggcgtcatcc ccatcaaacg cgcgttgagt ttatcctgca
     5581 tgtagccttt cagaatgccg ttctcaatca gcacgttgta ctggcctggc gtaccttcgt
     5641 catcaatcgc caccgaacca cggcgatcga ccatcgtgcc atcatcaacc acggtacaca
     5701 gttctgaagc caccaactcc ccgacctgtc cactaaatac tgaagtgcca cggcggttga
     5761 agtcgccttc cagaccgtga cccaccgctt catgcaacag cacgcccggc caacctgcgc
     5821 caagtactac cggcatggtg cccactggtg cagcaacggc agaaagattg accagcgcca
     5881 tacgcactgc ttcttttgcc cacgcatccg cacggacttc gccgtcgaga tcggcaagga
     5941 agaactcata accaaaacga ccgccgccac cactggcacc gcgttcgcgt ttgccatctt
     6001 cttcgaccaa aacgctcacg gaaagacgca ccagcggacg gacatccgcc gctagtgtgc
     6061 cgtcggtggc cgcaaccaga attaattcat agacaccact gaggctggca gtcacttcct
     6121 gtacgcgctt gtccgcttcg cgggcaacct tatcgacgcg acgcaggata tccaacttct
     6181 cttcacggct catgctttgc agcggatcta ccgaggtata taacgggcta tgctctaccg
     6241 cgcccagcgt ctgtactttg ccatcaccac tatcacggac gatggtgcgc gccgcttgtg
     6301 cactctgttc cagcgccagc aggctgattt ggtcagcata agcaaatccg gttttttcac
     6361 cactgattgc acgcacacca acgccctgat cgatgttgta agaaccatct ttaataatac
     6421 ggtcttctaa aacccaggat tcgtgatagc tcgactgaaa atagagatcg ccataatcaa
     6481 ggcgacgttc ggccagttga ccgaggatcg cgaacaagtc ctgatgtttc aggccgttcg
     6541 ccgctagcaa ttgttcactt accaggttca gactcatcgt ttttgctact cgttagttac
     6601 tgcagtagag gattttttac ggctgccgga tgcggcgtga acgccctgtc cggcctacgg
     6661 ttatgttctg gctcgttggc ctttggcaac gattatcata tgagattggg gcaattacgc
     6721 gccctcgtca aatcattgcg ctttttcttt acgcggttgt cgcaacactt cgttgatttg
     6781 cggatcgtcc agcggacccg aaatgtgata gcgcaaaatg gagactttgc tccacagcgg
     6841 tcccagcact ttactggcgg caaacactgc cgcgccaaca atggggttaa ccgcaaaggc
     6901 cgcagccaca cccaccgtcg cagaaatctc tggtgcgaca accgcttcca tattcaggtc
     6961 gcgacgtacc agatttaccg accctttcat ggcgatgtcc gcttccaggc catccaccag
     7021 cgtgtcgtcg gtgtgcataa cgccgtcttt aatccacgcg gtgctgcgaa tggagtcaaa
     7081 atagaaccct tcgccaaaag tgtctctgaa atcaaaacgc agcttacgca tcagggcatc
     7141 tacgctcaat aagcgcagca attgcccggc atgtccggta ttgatttcgg taatttcgcc
     7201 tttacccagt tgagtatgaa tgatgccatt caacgtcgcc tcatctggct gccagggtgc
     7261 tttgcgccag tgtaaatcgt aatccacatt aaatgacgac tggcgtatgg gcgtcgtgac
     7321 accaaaaaat tctgcggcgg catcaatttt ctgcccgcgc agttttcctt tcagcgaggt
     7381 tcgttcattc cccggattgt taacccattc accatcggca gtgagccgcg caaaaccagt
     7441 atcaatcagt ccattagtca gcgttaacgt atcgccagaa atggtgatat cactgtcaat
     7501 gcgaccgaat ttttgccccc agaaccagca ctctgcgcat cgtatttgtg cgtctggcca
     7561 gccacggaag ctaatgcgtt ccgttgtcgg gaaaggtgat gactgtgttg aatcaccacg
     7621 agttttcgcc acgcttgggt tgtaataaag atatttgata ttcgccagcc acggcgcgtt
     7681 attacgcatc gccagcgtgg cgttgatttc acgcccttgc gcctcaacca gggtgccatt
     7741 tgccgtcggt tgcgaaacaa tactcaggtt attccattgc tgatttccca gcgacaacat
     7801 aggcgtacgt aacgttatgt gttgtgggaa actcgctgca ccaccgacac tctccgccgc
     7861 gcctttctgg aacagggcca gccactcggc accattcatc ggcggcatat tgagttcaac
     7921 gccactttgt tccggcaacg gcgggagcgt tttactgtct gccgcccaaa tagcacgatc
     7981 gagcgtcagc ttttgaccga gcaaccagcg gctattgaaa tagttatccg caccagcctg
     8041 tccggttaat tcaaagctgt tgagattgcc atcaacctta acgtttaccg atagtggttc
     8101 acccgcaggt ttggctaacg gtgaaggtaa gtgactgctc acattcttca aatcgccgtt
     8161 cagctctacg ttatacgtcg cgccagcatg ataaggcagc tcaatgccca ctttaccatc
     8221 ccacgccacg ctgccactta atgcttcgtt caccgctgca ggcagaacgc cggttttcgc
     8281 tggttgccag ttaccgttga gattcaccgc gacctggtag gcttttgccc cttctttggt
     8341 ggaaaaatcc acgttcaacg gctgattaaa ccagcttgct gtcagtggtt cactttgcag
     8401 atcgccattg ataaagctga atttaccgct caaatttttc agggtgctgt cgagtggttt
     8461 gataaacaga ctgttattac gcagcgtcac ttcacctttc gcggttacca actcgccgtt
     8521 cagcgggata tcaagatgta agcgagcatt cacatcgccg tcgagctgga gttcttgcag
     8581 ggtcgcaccc agggaatctt tcagcggtgt ctcatcaaag taagggccaa cggctttacc
     8641 cggaccttta atgtcagcgt caatcagcag tttttctttt gagtagtcag ggatcactgc
     8701 ggtgagatta ctcgcgcgca cgccgcccag attaacgcca tcggttttca tccataaacc
     8761 gtcgttaata aagtccagtt caatatcaag gttagttaat gcaggccagt ccggctggaa
     8821 ggcaaacttc gcgttgcgca gcggcaccag cacttcaaac tgaccttcgt tgtgtttata
     8881 ggggaagagt tgcggattgc caccataaac cagcgtcgcg ttatccgctt caccgccctg
     8941 aatcgcgcca cttaagtaat caaccaggtc tttacccatc aagttttccg ggaaatagcg
     9001 ccaggcttgt gaaccatcat cggtactgat gccagccaga atacccagcc agggttcatc
     9061 gttagcaggt tgcaggtaac gaaaaccgcc gcgcgcatgg acggctttgg ctttaacgtc
     9121 aatattacgc ccatccagct ggaaaccttt gtcattgttc agccagctta tcgttgcctg
     9181 gccgtcggcg atttccagtg gcgcacggaa taccgtttcg taaggcatct ttgcctgctt
     9241 catcgacgca gtaagcaaac cattttctac gctgccggaa agcgtcccgg agaagtgttc
     9301 cgcacccggt aataatttcc attgcttcca ggccagatcg ctccacgatg cctgaaaacg
     9361 ggtcttgtct gccgcctgaa gcgggatatc cagcgccaga gtgttaatct tgccgctcgg
     9421 ttgtgtggag cgccaaacat cacccagtgc aggtgaaagt ttcgcgacca gcgggcgtac
     9481 gccctccagc cctgccagct ccagattact ggcacgaatc cggagttcgt cactgcgttt
     9541 attgtctttg ccgccaacgt cctgttccgg tatccaggcc agcgtcaatg ctccgctcgg
     9601 ccagggtttg ccgtccatcg tgatccgtgt atcgggaata gagaactgcc atcccggatt
     9661 ttcacgcgta atatgcgcag tcagattatc caccgacagc gtatgcgttt ccttctcgcc
     9721 caaccagctg gcaccgccct gtttcagcca gacgtcaccg ccggttacat cgcctttgtc
     9781 gatcgtcatc cagccttcaa gggagaactg tgccgtttcc agcgcaatat tgtcctgcat
     9841 ccatttaccg agccacggct tcaggtcgat gtcatccgcc tggagccaga cgcgaccatt
     9901 gcttaacaac ccctcatcat cgcgcaaatc catacgcacc tgcatcacgc cgtgctgtcc
     9961 ggtaaggctg gagaggctta ccaggccttc cgcacggtgt cgacgtggat cgttcagcca
    10021 ggtgagctgt gggatcgcca gctcggcgcg ctgaccggat ggcgtcagga aactgacttc
    10081 actgtcgcgg agatcgaaat ggtcaaattg acgaagaaac agatcgctga tgtgactggc
    10141 ttccaggcta tcattaccac cgccgctggt gatagaagtg ttggtgcgaa agcgcagctg
    10201 ccagaaagtg aggtcgcgaa actgccagcg catatgtaac aggctctgcc agacatccag
    10261 cgccagggta acgcgtttaa ccgaaaattc gccgccatct tttagttctg cacggatgtc
    10321 gtgcgcttca agcgtcggac caaaattctg ccagctggct gagagctgac tggcttctac
    10381 cggcatgcca gtcgcggatt ctattttgtt gaggatttcc ggacgccagg cgtcaagatg
    10441 cggtaaagca atacgcaggc cgctaaccag cagggcagcg atcacaacga gcgcggctcc
    10501 agtaagcagt aaaatccccg gcaatcgcct cacccgtcac tccttgtctg ctaaaaatgt
    10561 gactcaaaaa ccctttgccg gatggcggcc cagcatctgt ttacatcatt acgacgtcaa
    10621 actgctcctg gttatagagc ggttcaattt gtactttaac ctgtttgcca acgaaaattt
    10681 ccacttccgc cagcgagtgt gactcttcgc ctttcaaggc ttcggctact gccggagaag
    10741 catagaccag gaaacggtcg gagtcgtaag catggtggac acgaacaatc tcgcgcatga
    10801 tttcatagca taccgtttcc acggttttca ccgttccgcg accgtggcag gttgggcatt
    10861 cgttacacag tacgtgctca atgctttcgc gggtgcgttt acgcgtcatc tccaccagcc
    10921 ccagcgccga aaaaccatta acgctggttt tcacccgatc tttgctcaac gcctgctcca
    10981 gcgagtgcag cacgcggcga cggtgatctt cattattcat atcgatgaaa tcaataatga
    11041 taatcccgcc cagattgcgc aaccgtaact ggcgagcgat agcctgcgtc gcttcaatat
    11101 tggtattgaa aatggtgtcg tccagattgc gatgaccgac aaacgccccg gtattgatgt
    11161 ccacggtggt catcgcttcg gtctggtcga taatcagata accaccggat ttcagttcta
    11221 ctttgcgttc cagcgctcgc tggatttcgt tttcgacatc aaagagatcg aaaatcggct
    11281 ggcgtccggt gtaatgctcc agcttgctgg tcatctcggg aatgtactct gaggtaaact
    11341 ccagcaatgc ttcgtaagtc aggcgtgagt caacgcgaat gcggtcgagt tcggcgtcgg
    11401 cgaaatcacg cagaacacgc tgcgccagcg ccagttcgcc gtacagctga taacgggtct
    11461 gcgggcgttt tttacgctcc ataactttgg tccagacgcg tttcagataa gcagcatcgg
    11521 aggccagttc ggcctcgcca accccttccg ctgcagtacg gatgataaac ccgccctgct
    11581 cgtcgcaata ctctgcgacc acttttttca ggcgttcacg ttctgattcg ctttcaattc
    11641 gttgggatac cccaacgtga gaagcccctg gcataaacac cagatagcga gaaggcagcg
    11701 tgatatcggt ggtcaggcgc gcacccttag tgccaagcgg atctttcacc acctgcacca
    11761 tcagatcttg cccctgacgg accagttccg agatgtcgcg caccgtgaat tgcttttgtt
    11821 cttcacccgc cacacattcg gtgtgcggca tgatgtcaga tgcatgaaga aacgcggctt
    11881 tatccagccc aatatctaca aaagccgcct gcatacccgg aagtacacga cttacacgac
    11941 ccttgtagat attgcctact attccgcgtc gcgcctcacg ttcaatatga atttcctgca
    12001 gaataccgcc atcaatatac gccactcgag tttccgaagg cgttacgttt accaacaatt
    12061 cagccgtcat gtttatccct tttctcacgc agtgcgttaa aattacttaa taattcatac
    12121 gtttcaacca gcggtaagcc gactacggcg tgaaagctgc catttatctt cctgacaaaa
    12181 cagccaccca gcccctgaat accgtatgca cctgctttat ctaacggttc gccgctggcg
    12241 acatagcccg cgatgtcttc gtctgttaac gttctgaaag tcacatcggt gaccaccagg
    12301 caatcgagaa tgtgctggct gtcggccaac gccactgccg tcatcacctg atgggtctga
    12361 cccgataatt tgcgcaacat ctgcgccgca tgctctgcgt cgcgcggttt ctcgagcact
    12421 tctccattca ggataacgat agtatccgca cccagcaccg ggagatcctg cgccgtttgc
    12481 gcgacacccg cctgtgcttt ctcgcgcgcc agacgcacaa catactgctg cgcgctctcc
    12541 tgcggctgac gctgctcctc aatgcccgta acaatacgtt caaaggtcac gccaagttgc
    12601 gcaagtaact cctgacgacg cggagaaccg gaagctaaat acagagaagt catagaaacc
    12661 tttattgcac tgcaaactgc tgacggactt tacgcatcag caagaaaatc cacggccaga
    12721 gaacgccatt gactacacta ctccagaaca cttccggtct gaaagagacg ttaatcacta
    12781 aaaactctgc ccagaaaaca ataatatcca ccaccagcga aagcaacatg acgaccagcg
    12841 cctgctgcca taatgcgagg ttgcggaaaa gctggtattt cagcgccacc aggtaagcaa
    12901 tgatgctcat cgccaatacg cgtacgccaa gcgtcgagcc gctgatcaga tccagtatgg
    12961 cacccatcac aaaacctgtg cccacattta cgcgatgggg caaggccagg atccaataca
    13021 acaagatgag taacacccag tttggccgga aaacaatcag gttgtccggc catggcatga
    13081 tttgcagcaa cagcgcaatg aggaaagaga gccagattac ccagcgtccc tggctacgat
    13141 agctcgccac tattgtcctc ccggcgcacg cgcaggcggt tgagcaccac tttgcggcgg
    13201 cgtagccctt tgtggagagc gattagcagc gggctgtgtg ggcgcagcag gcgcagtaac
    13261 tgcatttcct gtcgccggtt gctgcggagt cggctgagtg atccccgttg ccggttcagg
    13321 taactttggc cccatcgcgt ctggcgaagg caatacctgc ggcatcatct gcatcagacg
    13381 ttcattagca acacgatgca cctcttccgg cgtcatcggg ttagcgccgt tacgatccgc
    13441 gccccacagc agcagcagat aacgcaaacg ttgcagtcct gcagtcggac gcgcctgaat
    13501 cacagtataa gcgcgctggg tatcgagttt tacggaagag acaaccgcga ccggatagcc
    13561 ttccgggaaa cgaccgccca gaccggaagt caccagcaca tcaccaacac gaatatccgt
    13621 attcgctggc agatgctcaa gctgcaaatc atccgtacaa ccgttaccgg ctgcaattac
    13681 gcggatatcg ttgcgcagca cctggattgg cagcgcgtgg gtcgcatcac aaatcagcag
    13741 cacgcggctg gtcagtttag cgacggccac cacctgacca acaacacctt tgtcgctgat
    13801 aaccggctgg ccttcataaa cgccattaac gctaccttta tcgataacaa cttgatcgct
    13861 ataaggatcg ttaaccgtgg agataacctg agtcaccatt ttctgctcat cctgacgcag
    13921 cggggaaccc agcagctcgc gcagacgcgc gttctcctgt ttgtattgtc caagcatcag
    13981 cagttcactg tttttcagca acagttcctg acgtaacgcc cggttttcaa gttctagttg
    14041 gtcacgcgag gccagcgtct gcgatacgcc atccagcaat tcacgaggag cattggaaac
    14101 aaagtagaaa ggactgacgg cggtatccat ataagtacgg atttgactga acgtccccag
    14161 gcggctgtcg gcaataataa tgccgagcgc caccagcacc gccagaataa ggcgaatctg
    14221 tagcgacggg ccacggctaa aaattggctt cataagttat gcgtattctc gtatcagacc
    14281 aggcagggta aacagacact tcccctgcct gcatccgatt actcttcgct gaacaggtcg
    14341 ccgccgtgca tgtcgatcat ttccagcgct ttgccgccac cgcgcgccac acaggtcagc
    14401 gggtcttcag caacaacgac tggaatgccg gtttcttcca ttaacaaacg gtcaaggtta
    14461 cgcagcagtg cgccaccacc ggtgagcacc atgccgcgct cggagatgtc ggaagccagt
    14521 tccggcgggc actgttccag tgcaaccatt accgcgctca caataccggt cagcggttcc
    14581 tgcagtgctt cgaggatttc gttggagttc agggtaaaac cgcgtggaac accttctgca
    14641 aggttacggc cacgaacttc gatttcacgg acttcatcgc ccgggtaagc cgaaccgatt
    14701 tcgtgcttga tacgttctgc ggtggcttca ccgatcagag aaccgtaatt acgacgtaca
    14761 tagttgatga tagcttcgtc gaaacggtca ccaccaatgc gcacggaaga ggagtaaacc
    14821 acgccgttca aggagataac agcaacttca gtagtaccac cgccgatatc aaccaccata
    14881 gaaccggtcg cttcagaaac cggcaggcca gcaccaattg cggcagccat cggttcttca
    14941 atcaggaaga cttcacgggc accagcgccc tgcgcggatt cacgaattgc gcggcgttca
    15001 acctgggtcg cgccaaccgg cacacaaacc agaacgcgcg ggcttggacg cataaagctg
    15061 ttgctgtgca cttgtttgat gaagtgctgg agcatttttt cagtcacgaa gaagtcggcg
    15121 ataacgccgt ctttcattgg gcgaatggca gcaatattgc ccggcgtacg gcccagcatc
    15181 tgcttcgcgt catgacctac tgcagctacg cttttcggtg aaccggcacg atcctgacga
    15241 atggccacca cggaaggctc attcaatacg atgccttgtc cttttacata aatgagggta
    15301 ttcgcagtac ccaggtcaat ggacaagtca ttggaaaaca tgccacgaaa ttttttcaac
    15361 atactaaggg ataatcctga aagctggggc ggaaaagaaa atccgcttac tttaccaacc
    15421 acacgcagca gcgacaaggc gcaaaaatca tctgctacgg tgaaaattag tgcagttcgt
    15481 ttcctttgtt acaaatctct gcctgagtcc agaaaggctt aatgcatcag cagcattcct
    15541 cgcctgtttg caaccgcgta aggtcattca tctgcatatg tgctgcaaca atctggcgag
    15601 cagacaagca cactcccatg agacgcagcg cgcgttattc tacgtgaaaa cggattaaac
    15661 ggcaggttaa accgagtatc tttgtgaata ttttttcacg ttagtatcaa gtggctgtga
    15721 ggacgcgaaa aaatcccctt gcccacctgt aacaccgcgc tgaatcaggg tctgccactc
    15781 gcttcgcgaa cgcacgccgg tggcgtaaac ctgggtgctg gtcccggagc aggcttccac
    15841 caggctttga accagcagct ggttctccgt tcgcttctca atgtttctga ccagccccgg
    15901 atggagcttg agtaactcaa cattaagttc tttgatccaa ctggtgctta ccagcgtcaa
    15961 accagcctgg ttgacggcta cccgtacccc taaagcattc actaaacgaa taacaggttg
    16021 taagcgactg atatgttgac caacatcggc ctctgcaagt tcaataatta tgcgtcttcg
    16081 ttgcgatttt tcacattgca ttaacgtatc gcgcagccaa cgctgaaaac gcgggcgaat
    16141 cagcgactca acggtaacct gaatcgccag attttcctct ggccagtaac gcaatagtgg
    16201 aataagacgg ctgattagca agcggtcata ctcttccgat aaaccaaact gcaagaccat
    16261 cggcatatac tctgccgagc taacctcttc attaccatcg aagatgcggc acatgagttc
    16321 acgatgatga acccggcctt cgcgagtaac cgccggtttt tgataaaggc gcgggccgcc
    16381 gcgactgagc atttgctcga taagcgtacg ccagcgaaca ttaccgcgtc ctttttcagg
    16441 caacgagtca tcgtaaatag cccagctatt gccgccctgc aatcccgcat tacgcgtggc
    16501 agactctgca tgttccatta cctgctcggt atcctgacca ctacgccagg cacagatacc
    16561 aatgtggatc atatcgtcgc gatcgagcat tttattgttc ggcaaggtat cgacggcttt
    16621 gattaactga ctggcgatgc tctctgcctc ttttagcgtc cggtgcggta acagcgcagc
    16681 aaaatcactg cgatggtaac gcgccagcag tgcgccaggg tagcgcatca taaatgtcga
    16741 tagcagattc gtcagagaga agaattgttc ttcaaccagg ctgtgccccc aggtatcact
    16801 caacatattg aaatccggca gacgaatcat catcacgatc ccgtgggtac ctactttctc
    16861 ttgatcttcc agtaacgttg ctaactgatt atcgaaaaag agtcggttat tgaggccggt
    16921 tttcatgtcc tgggcggcat aagagcggat cagcgtatca agacggctgt gttgttcgcg
    16981 tgcgttctga atttcacaaa gcagcgtatc cagcgcactg ctggttctgg gcggccattc
    17041 atagatggtt cccaacacat tagagccacg ctcaccgttt aagatacgag tagcccgggt
    17101 ttccagcaat tcttgcccgg caagttgccg ttgtaaccag cgtaccgcca ggaagagcat
    17161 aagaatgata aagccaatcg cccccgtgag cggcgcggtg gtcattaaag aatggaaata
    17221 gttgcccatc ggatcctgat aaaccagacg cagcgacatc cccggatgct ttatcaacgg
    17281 aacgctcagt tcacgaaaca gatcgttggt gccaaccgga cgataactac catttctggc
    17341 cagggtataa acctgcttat caccatggag caggtctaca cgaacgatat ctgccgacat
    17401 cattaattcg gtaatttgtg gccttaatgt gctgaagtca ttcgacagta ggtgggtatc
    17461 aatcgccgtc gccaccgcct gaacacgatg actaaactta tactgaatgg cgttgtagaa
    17521 acttagcgaa cagcccagca aagtcacaaa aattgttaac ccggtgagca gcgtgacaaa
    17581 ggccgaaaat ttcgtcgtta atctcatcct tgtgttaact ccgatagtga ggaagcgggc
    17641 atactagcaa atcagattta tctcgcaatt tattgcgctt catcggcttt gcttttccat
    17701 tagcgagtat agtcttcaga aattattttc caatccatca tgcacatgag gaccacttat
    17761 gcaggcgtta cttttagaac agcaagacgg caaaactctc gcatcagtac agactctgga
    17821 cgaaagtcgc ctgccggaag gcgatgtcac ggttgatgtt cactggtcga gcctgaacta
    17881 caaagatgcg ctggcgatta ccggtaaggg aaaaatcatc cgtaattttc cgatgattcc
    17941 tgggatcgat tttgccggaa ctgtacgcac cagcgaagat ccgcgttttc atgccggtca
    18001 ggaggtgtta ctcactggct ggggcgttgg tgaaaaccac tggggtgggc tggcggaaca
    18061 ggcgcgagtg aaaggtgact ggctggttgc catgccgcaa gggctggacg cgcgtaaagc
    18121 aatgattatc ggtactgccg gttttaccgc catgctgtgt gtgatggcgc tggaagatgc
    18181 cggtgttcgc ccgcaggatg gggagattgt cgtgacgggt gccagtggtg gcgtcggcag
    18241 taccgccgtg gcgctgctgc ataagttggg ttatcaggtc gttgctgttt ccggtcgcga
    18301 aagtacccat gaatatctga aaagtttagg tgccagccgt attcttcctc gcgatgagtt
    18361 tgccgaatcc cgtcctctgg aaaaacaagt ctgggctggg gcaattgaca ccgttggcga
    18421 caaagtgctg gcaaaagtgc tggcgcaaat gaattacggc ggctgcgtgg cggcctgtgg
    18481 tctggcgggt ggttttactc tgccaaccac ggtcatgcca tttattctgc gtaatgtccg
    18541 tttgcaaggg gtggattcag taatgacgcc accagcacgc cgtgcacaag cctggcagcg
    18601 actggtcacc gatttaccgg aatcattcta tacccaggcg gcaaaagaga tatctctggc
    18661 agaggcaccg aagtttgccg aggccatcat taataaccag atccagggtc gcacgctggt
    18721 gaaggttaac taaccattta gcagggaata ataagagagg gaactcattt tgaaatcatt
    18781 attcaatcag ttaacgggaa aagcggttag ccggacagct ttcgtcgaac atcttggtta
    18841 tgaagttgta caacatcatc caaactggaa agtcatgatt tcgactgacc ataaattgat
    18901 gcgcattgat gctccaccaa acagccatta ttgatacgcc tccgtcgcct gttaggttta
    18961 tggtgctttg ccttggcgac gctacgctta atctgttact gatttctggt accatggggg
    19021 gaataatctg attttatttg attacaaatt aaccacttaa acctattgaa tgcggagcat
    19081 tgtcagtcgg ttaattttgc gtgctttagc attcacatct ttccggacga tgcagtgaaa
    19141 attgggtaat ccccagcaac cgctgcgtaa tgtcgtctat cttgtcgcga tcctggcatc
    19201 cctacattat ttgtggtctg tgaagattat ctcattgcag cccccatctt cgcagggctg
    19261 gctttacagc gtttcacctt acgttataag aagttccgtc gatgatggcg ctaatttcgt
    19321 gaattgtgcg gcttgttgca aattacacgg tgttgaaggt tatttacatg ttagctgttg
    19381 attatcttcc ctgataagac cagtatttag ctgccaattg ctacgaaatc gttataatgt
    19441 gcgacctcgt cctccctgac gcagtttttg cactgcggaa aaggtgacat tggcgcaacg
    19501 aaggtatatt ttgttttttg ccggaggata gcagcagatc gctgcacaat gtccgtcaag
    19561 tctaacattg acactctggg gcaaaataga ccggcgtccc ggcctgctgg aatttatcgc
    19621 tatgcataca gctgtcgggg catacgcttt acagacggcg gtgaaacgcc tgtcacaatc
    19681 acactaaaca aagagtacgg aacccactca tggatattcg taagattaaa aaactgatcg
    19741 agctggttga agaatcaggc atctccgaac tggaaatttc tgaaggtgaa gagtcagtac
    19801 gcattagccg tgcagctcct gccgcaagtt tccctgtgat gcaacaagct tacgctgcac
    19861 ctatgatgca gcagccagct caatctaacg cagccgctcc ggcgaccgtt ccttccatgg
    19921 aagcgccagc agcagcggaa atcagtggtc acatcgtacg ttccccgatg gttggtactt
    19981 tctaccgcac cccaagcccg gacgcaaaag cgttcatcga agtgggtcag aaagtaaacg
    20041 tgggcgatac cctgtgcatc gttgaagcca tgaaaatgat gaaccagatc gaagcggaca
    20101 aatccggtac cgtgaaagca attctggtcg aaagtggaca accggtagaa tttgacgagc
    20161 cgctggtcgt catcgagtaa cgaggcgaac atgctggata aaattgttat tgccaaccgc
    20221 ggcgagattg cattgcgtat tcttcgtgcc tgtaaagaac tgggcatcaa gactgtcgct
    20281 gtgcactcca gtgcggatcg cgatctaaaa cacgtattac tggcagatga aacggtctgt
    20341 atcggccctg ctccgtcagt aaaaagttat ctgaacatcc cggcaatcat cagcgccgct
    20401 gaaatcaccg gcgcagtagc aatccatccg ggttacggct tcctctccga gaacgccaat
    20461 tttgccgagc aggttgaacg ctccggcttt atcttcatcg gcccgaaagc agaaaccatt
    20521 cgcctgatgg gcgacaaagt atccgcaatc gcggcgatga aaaaagcagg cgttccttgc
    20581 gtaccgggtt ctgacggccc gctgggcgac gatatggata aaaaccgcgc cattgctaaa
    20641 cgcattggtt atccggtgat tatcaaagcc tccggcggcg gcggtggtcg cggtatgcgc
    20701 gtagtgcgca gcgacgctga actggcacaa tccatctcca tgacccgtgc ggaagcgaaa
    20761 gcagctttca gcaacgatat ggtttacatg gagaaatacc tggaaaatcc tcgccacgtc
    20821 gagattcagg tactggctga cggtcagggc aacgctatct atctggcgga acgtgactgc
    20881 tccatgcagc gccgccacca gaaagtggtc gaagaagcac cggctccggg cattaccccg
    20941 gaactgcgtc gctacatcgg cgaacgttgc gctaaagcgt gtgttgatat cggctatcgc
    21001 ggtgcaggta ctttcgagtt cctgttcgaa aacggcgagt tctatttcat cgaaatgaac
    21061 acccgtattc aggtagaaca cccggttact gaaatgatca ccggcgttga cctgatcaaa
    21121 gaacagctgc gtatcgctgc cggtcaaccg ctgtcgatca agcaagaaga agttcacgtt
    21181 cgcggccatg cggtggaatg ccgtatcaac gccgaagatc cgaacacctt cctgccaagt
    21241 ccgggcaaaa tcacccgttt ccacgcacct ggtggttttg gcgtacgttg ggaatctcat
    21301 atctacgcgg gctacaccgt accgccgtac tatgactcaa tgatcggtaa gctgatttgc
    21361 tacggtgaaa accgtgacgt ggcgattgct cgcatgaaga atgcgctgca ggagctgatc
    21421 atcgacggta tcaaaaccaa cgttgatctg cagatccgca tcatgaatga cgagaacttc
    21481 cagcatggtg gcactaacat ccactatctg gagaaaaaac tcggtcttca ggaaaaataa
    21541 tttgtgacgc gcggtgtttt catcgcgctt agcaaagaac gtaatcaacg ccgggcctgt
    21601 taatgctacc cggcgttttt attgccgtta actaacggta gcctgcaatt tccgctttca
    21661 acgcgctaat ttcagcatcg ccatcaacaa ttcccagctc aaagcagtat ttctttgttt
    21721 cccctggctc aataaagggc agacgcccct gcgctcgttc tttatcacga ccgtcaggaa
    21781 aactattact gacctccagt cccataacat attcacccgc gcctaaattt ttccactgga
    21841 taaagttggg tagctgcatg gtgtcgaatt tctcgtaaat tccccacccc agttcggcgt
    21901 tcaccagcgc aactttactc atcccctgac tgtcgctatt caacgtgaga tagtaaacct
    21961 gctcgtcaaa accgggctgt ggggtacaaa tctccagatg actggttaac ccttccgccg
    22021 catgcggagt tcgtggcgtt atgcttttag ccgacagcaa gatttctgcc tgtggcgata
    22081 ataaaggcca gccgtaatta aagtgataaa gaatttgcag cggctggcgg gtgaaaccat
    22141 gattagtcac cttgtcttca atgcgcatta cattttcgcc ataacggcag cgaatggtcc
    22201 tttccagcgt cagattttca ccgaacaatc gtgtttcccg tactttgcca ttgatgacaa
    22261 attcaatgct gtcgtcggtc cgttctgtgc gataccccac ctcttctgct ggagtcgcag
    22321 ccaaacgccc gtgtaacccc agcgtttctc cttcatcctc acagggtgtg ccaatgtagc
    22381 taagtccgca ggtagtcagt aaccccgcgt aaaagctacg taaaaacccg ttgccctgag
    22441 actcaaaaaa agcgggggcg acaacaccgt tgggggtgat aaaagagagc gcagtgtcct
    22501 tgtagctggc ccatgcaata tccagcgcgc gatcttttac caccgtaaat gccagtcccg
    22561 caccagtacg gacatcaaac gcttctgtac cctttgctct gccactacgt aactcatagt
    22621 gtttaattcc ggcaacctgc gagagatcgc caatccacga ccaaatttcg tttttcattg
    22681 tgctgcttcc cgaatggaca tgtcatggcg ctggatgaac gtttcgattt ccataaggcg
    22741 agacgttccc tccattgggc cacgtcgggt taccgccagt gcaccgcagg cgttggcata
    22801 ttgtaatgcc cgatgggcgt caaaccctag ctggcggcag gcaatccacg cgccaccaaa
    22861 gcagtctcct gcaccggtcg ggtcaacttc ttccaccgga tagctctcaa catgaaactg
    22921 ctcattcgcg gagtagtaac tggctccttg attgccgcgc ttcacaatca cctctttaac
    22981 accttcttca agaaaaccag cgattgcacg ctccggcgtc gagtgtggcg acaagagcaa
    23041 cacttccccc tcactgggca tatagatatc cgttaactca agaacaaagt ggagggcatc
    23101 gcgcatttct ggaatatcaa gcatttcttt acgaatattc gggtcgaaag aaataacgcc
    23161 accatttgct ttaacgatgg ttactgcttt ttttactgcg tctaccatat ggaatgaaaa
    23221 cagggatgaa cccataatat gaaaatgggt acaatccttc agaatatttt catcaacatg
    23281 ctgtgccgat aatttaccgc aagcggcatt cttgatatta aaaataaaat cgcgatcgcc
    23341 agagttatga taagtaacaa aggcactacc ggtggcttcc agaggtaaga ctgaaatccc
    23401 cctgatgtcc acaccgtcag cagccagacg atgtatatta atatcaccaa agccatcatt
    23461 ccccacgcag ctaatgatgc cgcacggaac gccaagacgt gtcacctggt cgataaaaat
    23521 agcgggcgca ccactgggat aagggccgtt ccagatacct ggttgatcaa atggctggcc
    23581 gattttactc gccataattt caaccagaat ttctccaatt gtgaagacct tactcatatg
    23641 attaattcct tgaaatgatt agctgcgttt tcttttattt cttacgtcaa aataaacggc
    23701 tacaagaatg actaatcctt taaccaccat ctggtaaaaa taaggaacat tcattaagtt
    23761 catgccatta ttaataacgc cgatggttaa tgcgccgatc accgtcccca ccacggttcc
    23821 atagccgccg ttcaggctgg tgcctcccag tacagctgcg gcaatagcat ccagttcgta
    23881 gctcaacgcg gaattcggtt gcccggaata gaggcgtgag gcaaggatca tcccgctgat
    23941 acctgccagc acggctgtaa tcataaagac tttaattttc aagcgggtga catttatccc
    24001 tgaataaatt gccgcttctt tattaccacc agtgatataa atttttcgtc caaaggttgt
    24061 tttagtaaga atgaagtggt ttatcagcaa taagatgatt aatatccata taggaatagg
    24121 tatatagaga aattctccat tccccagcgc cagaaaggca tcatctttaa tcatgatggg
    24181 cataccatcg gtcactatat aagcgatacc acggaagatc cccatagtag caacggtgac
    24241 aataaacgaa gggatccccg ctatagccgt caagccacca tttatcatcc ccagagccag
    24301 tgctgcaata atggataatg ggaccaataa aacaaatgga attccccatg aagccccaag
    24361 cgcagcaaat gtgccaatca atgcaatggt tgaaccgacc gataaatcaa tatcgccaag
    24421 caaaagcaca taagtcattc cataggcggt aattgcaatt atcgaaacct gaagaataat
    24481 attagtaatg ttattggcgg taaaaaaact gtcattaata aaactgaaca gaatgattaa
    24541 caccaccatt ccactgagga taccaccata ttgcctgaag acttttttaa aattatttgc
    24601 aatgggaatt cccgaaggtt tcggactggc aagagttttc tctgtcattt aacgtactcc
    24661 tgttgcagcg gtcattacct gtgtctgcgt taaattttca catgaataaa tatcggcaat
    24721 agttttatta cgcattacca ccagacgatc gctaagtgcc atgacttctg gcagttcaga
    24781 ggacaccaga ataattgctg tccctgccgc agccagttga cgtatcacgt tatagatttc
    24841 aaattttgcg ccgacatcca ctccgcgcgt tggctcatcg agcaataaaa tacgcggagt
    24901 tttctccagc cacttcgata acacgatttt ctgttgatta ccgccgctca gtgctcccac
    24961 tgcctgatgc acagaactca ctttagtgtt cattctggca attgattgca gcgtcttcgc
    25021 ctcttcttgt cggtgattga tgagtcccat ccgcgaagcg ttatattgaa acgccacatg
    25081 gtgcgtgttc atattgacgt cgtgcatcag cactaaccct tcttccttgc ggttttcggt
    25141 cacaaaagca atgccatgat caatagcctg tttgggatta gcgatcctga cttccttacc
    25201 atcaatcaac acagtcccgt gatacgaaac gaggccaaat agcgccttca tcacatcgga
    25261 acgcccggcc cccaccaggc caaaaaatcc gaggacttcc cccggacgca cagcgaaact
    25321 gacctcttta aaaagcgaag gatgggaaag attttttact tccagttttg caggaacatt
    25381 ttcatccggt ttttccccaa gacgtgctgg ccaaatattt ttcatttccc gaccaaccat
    25441 ttgggcaatc aagtgctgga tatcactgtt tttcttttca tgcgtagcga tatactcacc
    25501 gtcgcggatc accgaaatat catctgaaat ccgcataatt tcatccatgc gatgagagat
    25561 ataaataacc gcacagcctc gctgctttaa tttttcaata atgttaaaca ggatctccgc
    25621 ttcattatca cttagcgaag atgtcggttc atccataatc acgacatccg ctgcatagct
    25681 taaggcttta gcaatttcta ccagttgcct atgtgccatc gacatattgc cgaccatttc
    25741 cagagggtct agtgggatgc ccagttccgc gaggaggttc tggcaatctg cgagcatttt
    25801 tttatcgtta atgatgccaa aacgtcgcgg ttcattattg gcataaatat tttccgccac
    25861 ggtcatatta ttactcaggc tcagttcctg aaaaataatc gcaatcccta actggcgaga
    25921 atgctgcgga ttattaatgg tgatggtttc accacgtagt gttatcgttc cctgggttgg
    25981 cggataaaca ccggccagta ttttcattaa tgtcgattta ccggcaccat tctcgcctaa
    26041 taaagtatgc acccgccctt tacgtaaaga gagtgtaata tcttttaatg cctgaacatg
    26101 accaaatgtc ttcgcaatat gcgaaacatc aagaataaca tccgtcataa tgacctcggt
    26161 gtcacaacaa aacgccaata acgttttgtt gtgaggtgaa gatatttact atttacctgt
    26221 ataaacgccg ggttgtaccg ggatcatttt ttctaccggt ttaccattga ggtgatcaac
    26281 agcggcatca atggctttag cacccatttg atctggatat tgccgaatca cggccgcaaa
    26341 cgttttttca ctgtcaacgg catcacgcgc ttctttcatg ccgtcaaaac caatgatttt
    26401 gatgttgtcg ttatgcgcag attttgctgc gataaccgct gccagcgccg catcatcacc
    26461 aaagccaaac agaccattca ggttagcatt tgcctgcata atgttttgcg cagtcgttaa
    26521 cgcttcagct cgcgtaatac caggctgttc agcaacaatt ttaatatccg gtgtatcgct
    26581 taaccctttc ttaaaaccat caacgcgcgc aaccaccgat tgtagtgccg gataagaaat
    26641 aatggctaca gtacctttac cactcaaaag tttacccatt aacttgcctg cttcaacgcc
    26701 tcccgcatag ttatcggtcg caacatgcga tacgaccgga acgccttccg cagcgacgtc
    26761 aacggtgata acgggaatac cggccttatc ggctttaatt atcgctgcct ggacgccttt
    26821 cgaatcgacc ggagagataa taatggcatc cactttttta gtaataaaat cctcaacatc
    26881 agaaagttgt ttgtttaaat cctgattggc aatagagaca cttaaattga cattgtcttt
    26941 ttttgcctct tctttcatgg cattcgcaag atcaatatag aaaggatgtt gttgtgttaa
    27001 taatgacgca ccaattttat actcttttgc caacgaactt tgcgagaaca atgccaggct
    27061 caccaacata gctgaaccaa gtaatttaat atttttcata aacaatcctc attatttttg
    27121 tagggtacaa cgtcttaaat ttttccttca ctaccacaaa cgccaataat acgagttacg
    27181 acttctttca tggcggcctt tgcgggctgc atatagtgac gtggatcatt tgccgcagga
    27241 ttgttgtaga aatatccttt taacgcatcg gcaaacgcaa ttttcaattc tgtcgcgaca
    27301 ttcactttac aaataccgcg acgaatacag tgctgaacgt cctgttccga taagcccgaa
    27361 gcaccatgca acaccagcgg aatctccact cgctgacgaa tctgttccag ccgcacaaaa
    27421 tccagtcgtg gttcatgcgc atataaacca tgtgctgtgc cgattgctac cgccagggag
    27481 tcaatttggg tacgttgaac aaactctgta gctgcggctg gcgaggtata agcggaatct
    27541 ttttcatcca cccgcagatc gtcctcctgg ccgccaagac gccctagctc ggcctcaacg
    27601 ctaacatcaa agcgatggca aaaggtggta acttcttgca ctcgcgcaat gttgtcttca
    27661 aatggcaaat gggagccatc aatcatgaca gaacggatcc ctgcgcgaac cttgttaaaa
    27721 atgtcatcac tctcttcatg atggtcgaga tgcaggacta acggcaggtt gtactcgcga
    27781 gccagcgatt gtactaacga aattaaactt cccacgccac cataacgata tgtaccgggt
    27841 gttcccgcca gcataacggg agagcgaagt tgtgcagcgc tctcaacaac tacctgtaaa
    27901 gtttcaaggt tatgaatgtt gaaagcaggg acggcatatc cctctgactg ggcttttttc
    27961 agcatgtttt tgcttgaaat aatgtacatc gcagctcctg ttatttgtcg ttcgccttac
    28021 aaaatgaaga aagagaatct tcactttcat tttgaaagca aactagcaca gcaaaagcag
    28081 caaaaagcgt gatgcatgtc gaacatcatc aaaataactt acaaaaaata acttaattta
    28141 tttttcactt tgcaagtgaa c
//
LOCUS       DACADF010000055        27940 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.055, whole genome shotgun sequence.
ACCESSION   DACADF010000055 DACADF010000000
VERSION     DACADF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.055"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            56..826
                     /locus_tag="ISB30_002215"
     CDS             56..826
                     /locus_tag="ISB30_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAP1989845.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            841..1782
                     /locus_tag="ISB30_002216"
     CDS             841..1782
                     /locus_tag="ISB30_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAP1989846.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            1833..3119
                     /locus_tag="ISB30_002217"
     CDS             1833..3119
                     /locus_tag="ISB30_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP1989847.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFNGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            3116..3403
                     /gene="fixX"
                     /locus_tag="ISB30_002218"
     CDS             3116..3403
                     /gene="fixX"
                     /locus_tag="ISB30_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAP1989848.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKRDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            3462..4793
                     /locus_tag="ISB30_002219"
     CDS             3462..4793
                     /locus_tag="ISB30_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1989849.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWTLPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            4901..5431
                     /gene="kefF"
                     /locus_tag="ISB30_002220"
     CDS             4901..5431
                     /gene="kefF"
                     /locus_tag="ISB30_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAP1989850.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            5424..7286
                     /gene="kefC"
                     /locus_tag="ISB30_002221"
     CDS             5424..7286
                     /gene="kefC"
                     /locus_tag="ISB30_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAP1989851.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRRAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFVLSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            7478..7957
                     /gene="folA"
                     /locus_tag="ISB30_002222"
     CDS             7478..7957
                     /gene="folA"
                     /locus_tag="ISB30_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAP1989852.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            8043..8276
                     /locus_tag="ISB30_002223"
     CDS             8043..8276
                     /locus_tag="ISB30_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP1989853.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            8279..8563
                     /locus_tag="ISB30_002224"
     CDS             8279..8563
                     /locus_tag="ISB30_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein CcdB"
                     /protein_id="HAP1989854.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLIDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIK"
     gene            complement(8590..9438)
                     /gene="apaH"
                     /locus_tag="ISB30_002225"
     CDS             complement(8590..9438)
                     /gene="apaH"
                     /locus_tag="ISB30_002225"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAP1989855.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLSE
                     GEAVAS"
     gene            complement(9445..9822)
                     /gene="apaG"
                     /locus_tag="ISB30_002226"
     CDS             complement(9445..9822)
                     /gene="apaG"
                     /locus_tag="ISB30_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAP1989856.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            complement(9825..10646)
                     /gene="rsmA"
                     /locus_tag="ISB30_002227"
     CDS             complement(9825..10646)
                     /gene="rsmA"
                     /locus_tag="ISB30_002227"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP1989857.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVGVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            complement(10643..11632)
                     /gene="pdxA"
                     /locus_tag="ISB30_002228"
     CDS             complement(10643..11632)
                     /gene="pdxA"
                     /locus_tag="ISB30_002228"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAP1989858.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTVGLLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELREQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            complement(11632..12918)
                     /gene="surA"
                     /locus_tag="ISB30_002229"
     CDS             complement(11632..12918)
                     /gene="surA"
                     /locus_tag="ISB30_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAP1989859.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            complement(12971..15325)
                     /gene="lptD"
                     /locus_tag="ISB30_002230"
     CDS             complement(12971..15325)
                     /gene="lptD"
                     /locus_tag="ISB30_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAP1989860.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDSPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            15580..16395
                     /gene="djlA"
                     /locus_tag="ISB30_002231"
     CDS             15580..16395
                     /gene="djlA"
                     /locus_tag="ISB30_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAP1989861.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGSGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            complement(16545..17204)
                     /gene="rluA"
                     /locus_tag="ISB30_002232"
     CDS             complement(16545..17204)
                     /gene="rluA"
                     /locus_tag="ISB30_002232"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAP1989862.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(17216..20122)
                     /gene="rapA"
                     /locus_tag="ISB30_002233"
     CDS             complement(17216..20122)
                     /gene="rapA"
                     /locus_tag="ISB30_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAP1989863.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(20287..22638)
                     /gene="polB"
                     /locus_tag="ISB30_002234"
     CDS             complement(20287..22638)
                     /gene="polB"
                     /locus_tag="ISB30_002234"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAP1989864.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPLLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQVKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKTLIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(22713..23408)
                     /gene="araD"
                     /locus_tag="ISB30_002235"
     CDS             complement(22713..23408)
                     /gene="araD"
                     /locus_tag="ISB30_002235"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP1989865.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGPIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(23523..25025)
                     /gene="araA"
                     /locus_tag="ISB30_002236"
     CDS             complement(23523..25025)
                     /gene="araA"
                     /locus_tag="ISB30_002236"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP1989866.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(25036..26736)
                     /gene="araB"
                     /locus_tag="ISB30_002237"
     CDS             complement(25036..26736)
                     /gene="araB"
                     /locus_tag="ISB30_002237"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAP1989867.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELCVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRNIGGIYSSEWFWAKI
                     LHVTRQDNAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEVWAQNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            27075..27920
                     /gene="araC"
                     /locus_tag="ISB30_002238"
     CDS             27075..27920
                     /gene="araC"
                     /locus_tag="ISB30_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAP1989868.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGIVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAHEWYHQWVYFRPRA
                     YWHEWVNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
BASE COUNT         6220 a         7339 c         7730 g         6651 t
ORIGIN      
        1 tgtaattaac accaccgata tgaacgacgt ttccttcatg atttctggag atgcaatgaa
       61 gattattact tgctataagt gcgtgcctga tgaacaggat attgcggtca ataatgctga
      121 tggttcgtta gacttcagca aagccgatgc caaaataagc caatacgatc tcaacgctat
      181 tgaagcggct tgccagctaa agcaacaggc agcagaggcg caggtgacgg ccttaagtgt
      241 gggcggtaaa gccctgacca acgccaaagg gcgtaaagat gtgctatcgc gcggtccgga
      301 tgaactgatt gtggtgattg atgaccagtt cgagcaggca ctgccgcaac aaacggcgag
      361 cgtacttgct gcagccgctc agaaagcagg ctttgatttg atcctctgtg gcgatggttc
      421 ttccgacctt tatgcccagc aggttggttt gctggtaggc gaaatcctta atattccggc
      481 agttaacggc gtcagcaaaa ttatctccct gacggcagat accctcaccg ttgagcgcga
      541 actggaagat gaaacagaaa ccttaagcat tccgctgcct gcggttgttg ctgtttccac
      601 tgatatcaac tccccacaaa ttccttcgat gaaagccatt ctcggcgcgg cgaaaaagcc
      661 agtccaggta tggtcggcgg cggatatcgg ttttaacgca gaggcagcct ggtcagaaca
      721 acaggttgcc gcgccgaaac agcgcgagcg tcagcgcatc gtgattgaag gcgacggcga
      781 agaacagatc gccgcatttg ctgaaaatct tcgcaaagtc atttaattac aggggatgct
      841 atgaacacgt tttctcaagt ctgggtattc agcgataccc cttctcgtct gccggaactg
      901 atgaacggtg cgcaggcttt agctaatcaa atcaacacgt tcgtcctcaa tgatgccgac
      961 ggcgcacagg caatccagct cggcgctaat catgtctgga aattaagcgg caaaccagac
     1021 gagcggatga tcgaagatta cgccggtgtc atggctgaca ctattcgcca gcacggcgca
     1081 gacggtctgg tgctgttgcc aaacacccgt cgcggcaaat tactggcggc gaaactgggt
     1141 taccgcctta acgcggcggt gtctaacgat gccagcgctg ttagcgtgca ggacggtaaa
     1201 gcgacagtga aacacatggt ttacggcggt ctggcgattg gcgaagaacg cattgccacg
     1261 ccgtatgcgg tactgaccat cagcagcggc acgttcgatg cggctcagcc agacgcgtca
     1321 cgcactggcg aaacgcacac cgtggagtgg caggctccgg ctgtggcgat tacccgcacg
     1381 gcaacccagg cgcgtcagag caacagcgtc gatctcgaca aagcccgcct ggtggtcagc
     1441 gtcggtcgcg gtattggcag caaagagaac attgcgctgg cagaacagct ttgtaaggcg
     1501 ataggtgcgg agttggcctg ttctcgtccg gtggcggaaa acgaaaaatg gatggagcac
     1561 gaacgctatg tcggtatctc caacctgatg ctgaaacctg aattgtacct ggcggtgggg
     1621 atctccgggc agatccagca catggttggc gctaatgcgt cgcaaaccat tttcgccatc
     1681 aacaaagata aaaatgcgcc gatcttccag tacgcggatt acggcattgt tggcgacgcc
     1741 gtgaagatcc ttccggcact gaccgcagct ttagcgcgtt gatccactct ggcagggctg
     1801 cattccggcc ctgccgctga cagggagctc ttatgtccga agatatcttt gacgccatca
     1861 tcgtcggtgc agggcttgcc ggttcggttg ccgcactggt gctcgcccgc gaaggtgcgc
     1921 aagtgttagt tatcgagcga ggcaattccg caggtgccaa gaacgtcact ggcgggcgtc
     1981 tctatgccca cagtctggaa cacattattc ctggtttcgc tgactccgcc cccgtagaac
     2041 gcctgatcac ccatgaaaaa cttgcgttta tgacggaaaa gtcagcgatg actatggact
     2101 actgcaatgg cgacgaaacc tctccatccc agcgttctta ctccgttttg cgcagtaaat
     2161 ttgatgcctg gctgatggag caggccgaag aagcgggcgc gcagttaatt accgggatcc
     2221 gcgtcgataa cctcgtacag cgcgatggca aagtcgtcgg tgtagaagcc gatggcgatg
     2281 tgattgaagc gaaaacggtg atccttgctg atggggtgaa ttccatcctt gccgaaaagc
     2341 tggggatggc aaaacgcgtt aaaccgacgg atgtggcggt tggcgtgaag gaactgatcg
     2401 agttaccgaa gtcggttatt gaagaccgtt ttcagttgca gggtaatcag ggcgcggctt
     2461 gcctgtttgc gggatcaccc accgatggcc tgatgggcgg tggcttcctt tataccaatg
     2521 aaaataccct gtcgctgggg ctggtttgtg gtctccatca tctgcatgac gcaaaaaaat
     2581 cggtgccgca aatgctggaa gatttcaaac aacatccggc cgttgcaccg ctgatcgcgg
     2641 gcggcaagct ggtggaatat tccgctcacg tagtgccgga agcaggcatc aacatgctgc
     2701 cggagctggt cggtgacggc gtattgattg ccggtgatgc tgccggaatg tgtatgaacc
     2761 tcggttttac cattcgcggt atggatctgg cgattgccgc cggggaagca gcagcaaaaa
     2821 ctgtgctttc agcgatgaaa agcgacgatt tcagtaagca aaaactcgcg gaatatcgtc
     2881 agcatcttga gagtggcccg ctgcgcgata tgcgcatgta ccagaaacta ccggcgttcc
     2941 ttgataaccc acgcatgttt aacggctacc cggaactggc ggtgggcgtg gcgcgtgacc
     3001 tgttcaccat tgacggcagt gcgccggaac tgatgcgcaa gaaaatcctc cgccacggca
     3061 agaaagtggg cttcatcaat ctgatcaagg atggcatgaa aggagtgacc gttttatgac
     3121 ttctcccgtc aatgtggacg tcaaactggg cgtcaataaa ttcaatgtcg atgaagagca
     3181 tccgcacatt gttgtgaagg ccgatgccga taaacaggcc ctggagctgc tggtgaaagc
     3241 gtgccccgca ggtctgtaca agaagcggga tgacggcagt gtgcgcttcg attacgccgg
     3301 atgtctggag tgcggcacct gtcgcattct ggggctgggg tcggcgctgg aacagtggga
     3361 atacccgcgc ggcacctttg gtgtggagtt ccgttacggc tgatgttggt ttgatacgta
     3421 atgccgtact gactctcatt gcaaaaaaac aggaataacc catgcaaccg tccagaaact
     3481 ttgatgatct taaattctcc tctattcacc gccgcatttt gctgtgggga agcggtggtc
     3541 cgtttctgga tggttatgta ctggtaatga ttggcgtggc gctggagcaa ctgactccgg
     3601 cgctgaaact ggacgctgac tggattggct tgctgggcgc gggaacgctc gccgggctgt
     3661 tcgttggcac atcgctgttt ggctatatct ccgataaagt cggacggcgc aaaatgttcc
     3721 tcattgatat catcgccatc ggcgtgatat cggtggcaac gatgtttgtc tcatcccccg
     3781 tcgaactgtt ggtgatgcgg gtacttatcg gcattgtcat tggtgcggat tatcccatcg
     3841 ccacctcaat gatcaccgag ttctccagta cccgtcagcg ggcgttttcc atcagcttta
     3901 ttgccgcgat gtggtatgtc ggcgcgacct gcgccgattt ggtcggctat tggctttatg
     3961 atgtggaagg cggctggcgc tggatgctgg gtagcgcagc gattccctgt ttgttgattt
     4021 tgattggtcg attcgaactg cctgaatctc cccgctggtt gttacgcaaa gggcgagtaa
     4081 aagagtgcga agagatgatg ataaaactgt ttggcgaacc ggtggctttc gaggaagagc
     4141 agccgcagca aacccgtttt cgcgatctgt ttaatcgccg ccattttcct tttgttctgt
     4201 ttgttgccgc tatctggacc tgccaggtga tccccatgtt cgccatttac acctttggcc
     4261 cgcaaatcgt tggtttgttg ggattggggg ttggcaaaaa cgcggcactg gggaacgtgg
     4321 tgattagcct gttctttatg ctcggctgta ttccgccgat gctgtggcta aacactgccg
     4381 gacggcgtcc attgttgatt ggcagctttg ccatgatgac gctggcgctg gcggttttgg
     4441 ggctgatccc ggatatgggg atctggctgg tagtgatggc ctttgcggtg tatgcctttt
     4501 tctctggcgg gccgggtaat ttgcagtggc tctatcctaa tgaactcttc ccgacggata
     4561 tccgcgcctc tgccgtgggc gtgattatgt ccttaagccg tattggcact attgtatcga
     4621 cctggacact gccgatcttt atcaataatt acggtatcag taacacgatg ctaatggggg
     4681 cgggtatctc gctatttggc ttgttgattt ccgtagcgtt tgccccggag actcgaggga
     4741 tgtcactggc gcagaccagc aatatgacga tccgcgggca gagaatgggg taatttgttc
     4801 agatttctct cttttctgaa tcaatattat tgactataag ctgcgtgaat atatgactac
     4861 actttgtggg aaaacaaagg cgtaatcacg cgggctacct atgattctta taatttatgc
     4921 gcatccatat ccgcatcatt cccatgcgaa taaacggatg cttgaacagg caaggacgct
     4981 ggaaggcgtc gaaattcgct ctctttatca actctatcct gacttcaata tcgacattgc
     5041 cgccgaacag gaggcgctgt ctcgtgccga tctgatcgtc tggcagcacc cgatgcagtg
     5101 gtacagcatt ccaccgcttc ttaaactttg gatcgataaa gttttctcgc acggctgggc
     5161 ttacggtcac ggcggcacgg cgctgcacgg caaacatttg ctgtgggcgg tgacgaccgg
     5221 cggcggggaa agccattttg agattggtgc gcatccgggc tttgatgtgc tgtcgcagcc
     5281 gctacaggcg acggcaatct actgcgggct gaactggctg ccaccgtttg ccatgcactg
     5341 cacctttatt tgtgacgacg aaaccctcga agggcaggcg cggcactata agcaacgtct
     5401 gctggaatgg caggaggcac atcatggata gccatacgct tattcaggcg ctgatttatc
     5461 tcggttcggc ggcgctgatt gttcccattg cggtacgtct cggtctggga tcggttcttg
     5521 gctacctgat cgccggctgc attattggcc cgtgggggct gcgactggtg accgatgccg
     5581 aatctattct gcactttgca gagattggcg tggtgctgat gctgtttatt atcggcctcg
     5641 aactcgatcc acaaaggctg tggaagctgc gtgcggcagt gttcggcggt ggcgcattgc
     5701 agatggtgat ttgcggcggc ctgctggggc tgttttgtat gttacttggg ctgcgctggc
     5761 aggtcgcaga attgatcggc atgacgctgg cgctctcatc aacggcgatt gccatgcagg
     5821 cgatgaatga acgcaatctg atggtgacgc aaatgggccg cagagccttt gcggtgctgc
     5881 tgttccagga tatcgcggcg atcccactgg tggcgatgat cccgctactg gcagcgagta
     5941 gtgcctcgac gacgatgggc gcatttgttc tctcggcgtt aaaagtggcg ggcgcgctgg
     6001 tgctggtggt attgctgggg cgctatgtca cgcgtccggc actgcgtttt gttgcccgct
     6061 ctggcttgcg ggaagtgttt agtgccgtgg cgttattcct cgtgtttggc tttggtttgc
     6121 tgctggaaga ggtcggcttg tcgatggcga tgggcgcgtt tctggcgggc gtactgctgg
     6181 caagctcgga ataccgtcat gcgctggaaa gcgatatcga accatttaaa ggtttgctgc
     6241 tggggctgtt tttcatcggc gttggcatgt cgatagactt tggcacgctg cttgaaaatc
     6301 cgttgcgcat tgtcattttg ctgctcggtt tcctcatcat caaaatcgcc atgctgtggc
     6361 tgattgcccg gccgttgcag gtgccaaata aacagcgtcg ctggtttgcg gtgctgttag
     6421 ggcagggcag tgagtttgcc tttgtagtat ttggtgcggc gcagatggcg aatgtgctgg
     6481 agccagagtg ggcgaaatcg ctgaccctgg cggtggcact gtcgatggcg gcaacgccga
     6541 ttctgctggt gatcctcaat cgccttgagc aatcttctac tgaagaggcg cgtgaagccg
     6601 atgagatcga cgaagaacag ccgcgggtaa tcattgccgg attcggtcgt tttgggcaga
     6661 taaccggacg tttactgctc tccagcgggg tgaaaatggt ggtgctcgat cacgacccgg
     6721 accatatcga aacgctgcgt aaattcggta tgaaagtgtt ttatggcgat gccacgcgga
     6781 tggatttact ggaatctgcc ggagcggcga aagcggaagt gctgattaac gccatcgacg
     6841 atccgcaaac caacctgcaa ctgacggaga tggtgaaaga acatttccca catttgcaga
     6901 ttattgcccg cgcccgcgat gtcgaccact acattcgttt gcgtcaggca ggcgttgaaa
     6961 agccggagcg tgaaaccttc gaaggtgcgc tgaaaaccgg gcgtctggca ctggaaagtt
     7021 taggtctggg gccgtatgaa gcgcgagaac gtgccgatgt gttccgccgc tttaatattc
     7081 agatggtgga agagatggca atggttgaga acgacaccaa agcccgtgcg gcggtctata
     7141 aacgcaccag cgcgatgtta agtgagatca ttaccgaaga ccgtgaacat ctgtcattaa
     7201 ttcaacgaca tggctggcag ggaaccgaag aagggaaaca tactggcaat atggcggatg
     7261 aaccggaaac gaagccctca tcctaataaa gagtgacgta aatcacgctt tacagctaac
     7321 tgtttgtttt tgtttcattg taatgcggcg agtccaggga gagagcgtgg actcgccagc
     7381 agaatataaa attttcctca acatcatcct cgcaccagtc gacgacggtt tacgctttac
     7441 gtatagtggc gacaattttt tttatcggga aatctcaatg atcagtctga ttgcggcgtt
     7501 agcggtagat cgcgttatcg gcatggaaaa cgccatgccg tggaacctgc ctgccgatct
     7561 cgcctggttt aaacgcaaca ctttaaataa acccgtgatt atgggccgcc atacctggga
     7621 atccatcggt cgtccgttgc caggacgcaa aaatattatc ctcagcagtc aaccgggtac
     7681 ggacgatcgc gtaacgtggg tgaagtcggt ggatgaagcc attgcggcgt gtggtgacgt
     7741 accagaaatc atggtgattg gcggcggtcg cgtttatgag cagttcctgc cgaaagcgca
     7801 aaaactgtat ctgacgcata tcgacgcgga agtggaaggc gacacccatt tcccggatta
     7861 cgagccggat gactgggaat cggtattcag tgaattccac gacgccgatg cgcagaactc
     7921 tcacagctat tgctttgaga ttctggagcg gcggtaattt cgtatattag aattccggca
     7981 tcactgatac gtgcagatat ccgtgtatac tagacgtata aattgtacag gagcacgaga
     8041 tcatgactgc aaaacgtacc acacaaagtg tgaccgtcac cgtcgaccgt gagttagtca
     8101 atcgcgctcg tgatgcaggc ttaaatatga gcgccaccct tacggttgcg ctcaatgctg
     8161 aacttaaaaa acatgcagca acacgttggc gtgaagagaa cgcagaagct atcgctgcgt
     8221 taaatcaatt ggctgatgaa accggatgtt tctctgatga gtaccggagc ttctagcgat
     8281 gcaatttacg gtataccgca gtcgcggcag gaacgccgcg tttccctttg ttattgatgt
     8341 taccagcgac attattggtg agattaatcg ccgtatcgtt attccattaa cacctattga
     8401 acgatttagc cgtattcgcc caccagaacg gcttaaccca atccttttac tgatagacgg
     8461 caaagaatat gtgctcatga cgcatgagac tgcaactgtt ccagttaacg cgctgggaac
     8521 gaaattttgc gatgccagcg cgcaccgaac gttgattaaa tgagcattag attttatgct
     8581 cgacgggatt taagacgcta ccgcctctcc ctcactcaaa tccttatgcc ggttcgacgg
     8641 ctggacaaaa tactgtttat cttcccagcg caggcaggtt aatgtaccac cccagcagca
     8701 gccggtatcc agcgcgtaaa taccttccgg cgtacctttg ccttccagcg atgcccagtg
     8761 accaaaggcg atgttgtact cttcagcgac ggggccagga atcgcaaacc acggtttcag
     8821 tggggcaggg gcctcttccg gcgattcttt gctgtacata tccagttgac cgttcgggaa
     8881 gcaaaaacgc atacgggtaa aggcgttggt gataaaacgc agtcttccca gcccccgcaa
     8941 ttccggtgac cagttatttg gcatatcgcc gtacatggca tcaaggaaga agggatagga
     9001 gtcactcgac agcaccgctt ctacatcgcg cgcgcactct ttggcggtct gcagatccca
     9061 ctgcggcgtg atcccggcgt gggccatcac caactttttc tcttcgtcga tttgcagcaa
     9121 aggctggcga cgcagccagt taagcagctc gtcggcatcc ggcgcttcca gcagcggtgt
     9181 caggcgatct ttcggtttat tgcggctgat cccggcaaat accgccagca gatgcagatc
     9241 gtgattaccc agcaccagac gtacgctgtc gcctaaggat ttcacatagc gcagaacatc
     9301 cagcgaaccc gggccgcgcg cgaccagatc gcccgtcagc cagagggtat ctttcccagg
     9361 ggtaaattct actttatgca gcaatgcgat cagttcatcg taacaaccat gaacgtcgcc
     9421 aataaggtat gtcgccatat tcttttaatg aatgagtgtg ggaacggcga gtcggaatac
     9481 gggaatgtcg atgctgaaag ggacgccatt ttcatcgatc atttcgtagt gaccctgcat
     9541 ggtgcccagc ggggtttcaa tgattgcacc gctggtgtac tggtactctt cgccaggcgc
     9601 gataagtggc tggacgccaa ccactccttc gccctggact tcggtttcac ggccattgcc
     9661 attggtgatc agccagtaac gccccaacaa ctgcactggc gctcgcccca gattgcgtat
     9721 ggttacggta taagcaaaaa cgtaacgttc attatcaggt gaagattggg cctcaatgta
     9781 gacgctttga acctgaatac acactcgggg cgaattgatc atcgttaact ctcctgcaaa
     9841 ggcgcgttct ccgccagata gttcgccatc tggcaatatt gcgcgacaga gatattttcc
     9901 gctcgcatcg ccgggtcgat ccccattccc gttaacaccc cgacgctaaa caggttgccg
     9961 aggctgttac gaatggtttt acgacgctgg ttaaaggctt cggtggtgat gcggctcaac
    10021 acgcgaacat ctttaaccgg gtgaggcatc gttgcatgag gaaccaggcg cacgacggcg
    10081 gaatccactt tgggtggtgg tgtaaaggct gacggcggta cttccagcac cgggatcacg
    10141 ttgcaatagt attgcgccat gacgcttaat cgaccatacg ccttgctgtt cggtcctgca
    10201 accagacgat tcaccacttc tttttgcaac ataaagtgca tgtcggcaat ggcatcagta
    10261 tagctaaaca gatggaacat caacggcgtg gagatgttat aaggcaggtt gccgaaaaca
    10321 cgcagcggct ggcccatttt cgcggccagt tcaccaaagt taaaggtcat cgcatcctgc
    10381 tgataaatcg tcagtttcgg gcctaagaat ggatgcgttt gcagacgtgc cgccagatcg
    10441 cggtcaagtt cgatgaccgt cagctggtcc agacgttcgc cgaccggttc ggtcaatgcc
    10501 gccagaccgg ggccgatttc gaccatcgcc tggcccttct gcgggttaat ggcagaaaca
    10561 atgctgtcga tcacgaactg atcgttgaga aagttttgcc cgaagcgttt acgggctaag
    10621 tggccctggt ggactcgatt attcattggg tgttaacaat cattttgatg gcgagattaa
    10681 gcgccgtaat aaaactgccg acatcggctt taccacgtcc cgccagttca agcgcggtgc
    10741 cgtggtccac tgatgtgcga ataaagggca ggcccagcgt aatgttcaca ccgcgcccga
    10801 agccctggta ttttagcacg ggaagaccct gatcgtggta catcgccagc acggcgtcgg
    10861 cgttatcgag atatttcggc tgaaacaggg tatcggcagg cagcggcccg ttgagtttca
    10921 tcccctgttc ccgcagctcg tcgagtaccg gaataatggt gtctatctct tccgtaccca
    10981 tatgaccgcc ttcgcccgcg tgcggattca gcccgcagac cagaatgcgc ggttcggcaa
    11041 taccaaattt ggtccgcaaa tcgtgatgca aaatagcaat cacttcgtgc aaaagggcag
    11101 gggtgatagc atctgcgata tcgcgcagcg gtaaatgcgt cgttgccagc gccacgcgaa
    11161 gttcttcggt cgccagcatc atcaccactt ttttcgcctg cgaacgctct tcgaaaaact
    11221 cggtatgacc ggtaaacgga atgcctgcgt cgttaatgac gcctttatgc acggggcctg
    11281 tgatcagcgc agcaaattca ccgttcagac agccatcaca cgctcgcgcc agcgtttcca
    11341 ccacatagtg cccattttca accgctaaca gccccacagt gacagattca cgtagcgcga
    11401 caggaagtag cgttaatgtg cccgcagttt gcggttgtgc aggggagttg ggggaataag
    11461 ggcggagggt gagcggcaaa ccgagcatcg ctgcccggtc ggtaaggaga gtggcatcgg
    11521 cacaaacaac cagttcgacc ggccactcac gctgtgcaag ctggacaatt aagtccgggc
    11581 caatcccggc gggctcgccg ggagtgatca caacacgttg ggttttaacc attagttgct
    11641 caggatttta acgtaggcgc tggcacgttg ttcctgcatc cagcttgctg cttcttccga
    11701 gaacttacgg ttcatcagca tgcggtatgc acgatctttc tgtgccgcgt cggttttatc
    11761 gacattacgg gtatccagca gttcgattaa atgccagccg aatgaagagt gaaccggtgc
    11821 actcatttga cctttgttca ggcgggtcag ggcgtcacgg aaggccggat cgaaaatatc
    11881 tggtgtagcc cagccgagat caccgccctg gttagcagag cctggatcct gagagaactc
    11941 tttcgctgcg gcagcaaaag tcgttttacc actcttgata tcggcagcaa tctgttccag
    12001 tttcacgcgg gcctgttcgt cagtcatgat cggcgacggt ttcagcagaa tatggcgagc
    12061 atgaacttcg gtcaccgaga tatttttgct ttcgccgcgc aggtcgttaa ctttcagaat
    12121 atggaagcca acgccggaac gaatcgggcc aacaatgtcg cctttcttcg cggtgcttaa
    12181 tgcctgggcg aagatccccg gcaactcctg aatacggccc cagcccatct ggccgccgtt
    12241 cagcgcctgc tggtcggcag aatgagcaat cgccagctta ccgaaatcag cgccgttacg
    12301 cgcctgatcg acaatcgcgc gcgcctggct ttccgcttcg ttcacctgat cagaggtcgg
    12361 gttttccggc agcgggatca ggatgtggct caggttcagc tcagtgctgg cgtcgttttg
    12421 gttacccact tgctgcgcca gggattcgac ttcctgcggc aggatggtga tgcgacgacg
    12481 cacctcgttg ttacgcactt cagagataat catctctttg cggatctggt tacgataggt
    12541 gttgtagttc agaccatcgt aagccagacg gctgcgcatc tgatccagcg tcatgttgtt
    12601 ctgtttcgcg atgttagcaa tcgcctgatc cagctgctca tcggagattt tcactcccat
    12661 tttctgcccc atctgcagga taatttgatc catgatcaaa cgttccatga tttggtggcg
    12721 cagcgtcgcg tcatcaggaa gttgttgcct tgcctgagca gcgttcagtt ttaccgactg
    12781 cattaatcca tcaacgtcgc tttccagcac gacgccgtta ttgacgacgg ctgcgacttt
    12841 atcgactacc tggggggcag cgaaactggt attcgcgatc atggcgatac cgagaagcag
    12901 cgttttccag ttcttcatac tttttccatt tcaattaacc gcactgcgga ttacgtggta
    12961 aatcaacaaa tcacaaagtg ttttgatacg gcagaatgtt cgaacgcagc atctcttgcg
    13021 tacccagacc gtagttggag ctcaggccgc gaagttcgat gttaaagccg attgcgttgt
    13081 catataccgc atgttgttta tcgttatccc aaccgttcag cttccgctcg taaccgacgc
    13141 gaatcgcata gcagcaggag ctgtattgca cacctaacat agagtcggct tgcttgttcg
    13201 cattggtgtc gtagtagtag gccccaacaa tggaccaacg atcggcaatt ggccagctgg
    13261 cgacggcacc tacctgcgaa ataccattct tatattgctc agcagtggaa tagtacttag
    13321 gcagcgtagc ctgaatatat tccgggctgg cgtaacggta attcagctgt accagacggt
    13381 cttcatcacg acggtattca atgctggagt tactggtcgc tacgttatcc agacgtgtat
    13441 cgtactgaat cccgccacgc aatccccaac gatcggagat gcgccagtaa gtatcgcctg
    13501 cccataccag tgagcccgtt ttgtcgtcat tctcccatgt tatgttgtca tcgccagtgc
    13561 gagactccgt gaaatagtag atttgaccaa cggaaatatt aaaacgttca acggcagcat
    13621 catcatatat gcgagatgtg acaccggtcg tcacctggtt agcggaggca atacggtcaa
    13681 gaccgccgta agtccggtcc cggaacaggc cggagtagtc agattgcagc agcgaagagt
    13741 cgtagttata gattttgctc tgatcgcgat atggcacata cagatactgc gcgcgtggtt
    13801 ccagtgtctg ggtatagccc ggcgccagca tttccatatc acgctcgaag accattctac
    13861 cgtctacctt gaactgcggc attacacggt tagcggactc tgccagcctg gtcgtgtttc
    13921 tggagttata ccagtcaaga ttggtttgct gataatgggt tgccagcaac ttcgcttcgg
    13981 tattgatgct gccccagtta ttagagagcg gcagattgat ggtcggttcc aggtgaacac
    14041 gggttgcttc aggcatgtcg tctctggtgt taacaaagtg cactgcctga ccgtaaatac
    14101 gcgtatcaaa cggaccaaca tcattctggt agtagttaac gtctaactgc ggctctgccg
    14161 agtagctact ggtgttctgc tcgctgaaga cctggaactg cttggttgaa acggtggcat
    14221 tgaagttttg caccgcatag ccaacgctga atttttgcgt tgcgtagccg tcggtactgg
    14281 aaccgtattt gttatcgaag tcattgaagt agctaggatc gctgactttg gtgtagtcga
    14341 cgttgaaacg ccacacctga tccatgaccc cggagtggtt ccagtagaac aaccaacgac
    14401 gtgaactgtc atcgttcggg tgttcatctt tatagacttt atctgaaggc agatagtcca
    14461 gttccatcaa gccagcgccc gcctgggaga ggtagcggaa ttcgttctcc cacatgatgt
    14521 tgccacgacg atgcatataa tgcggcgtga tggtggcatc catatttggc gcgatgttcc
    14581 agtaatatgg caggtagaac tcaaagtagt tggtggtggt gtacttggcg ttcgggatca
    14641 agaaaccaga gcgacgtttg tcacccaccg gcaactgcaa atacgggcta taaaagatcg
    14701 gtaccggacc caccttaaag cgggcgttcc agatctccgc aacttgttct tcgcggtcat
    14761 gaataatttc gctacctacc acgctccagg tgtcagaacc cggcagacag gaggtaaagc
    14821 taccgttatc cagaatggta tagcggtttt cgccacgttg tttcatcagg tccgctttac
    14881 cgcgcccctg gcgacccacc atctggtaat caccttccca gacgttggta tctttggtgt
    14941 tcagattcgc ccagcctttc ggccctttga ggatcacctg gttatcgtcg taatggacat
    15001 taccgagcgc atcaacggta cgtaccggct ccggttgtcc tggtgcctct ttttgatgga
    15061 gctgcacttc gtcggcctgc agacggctgt taccctgcat gatatccacg ctgccagtaa
    15121 acacggcgtc atccggggag tcccctttcg cgtggtcagc attgatagtc acgggtaagt
    15181 cattggtatc gccctgtacc agaggacggt catagcttgg cacgcccaac atgcactgtg
    15241 aggcgaggtc ggctgccagt ccctgttgac tataaagggc ggtggcaatc atggtggcca
    15301 ggagagtggg gatacgtttt ttcatacgtt gattttattg ttccatcatc ggtaacgttg
    15361 cgcgtgacaa acggtcagag actaacgtac tcgtcatctc tacgctagtg ttaatcctgt
    15421 ccgaatagcg tcagtggtgt taggcacggc attgaatgac aggtatgata atgcaaatta
    15481 taggcgatgt cccacaattg accgcagccg gaaaacggta aaagcacctt tatattgtgg
    15541 gagatagccc tgatatccgt gtgtcgattt ggggaatata tgcagtattg gggaaaaatc
    15601 attggcgtgg ccgtggcctt actgatgggc ggcggctttt ggggcgtagt gttaggtctg
    15661 ttaattggcc atatgtttga taaagcccgt agccgtaaaa tggcgtggtt cgccaaccag
    15721 cgtgagcgtc aggcgctgtt ttttgccacc acttttgaag tgatgggcca tttaaccaaa
    15781 tccaaaggtc gcgtcacgga ggctgatatt catatcgcca gccagttgat ggaccgaatg
    15841 aaccttcatg gcgcttcccg tactgcggcg caaaatgcgt tccgggtggg aaaatcagat
    15901 aattacccgc tgcgcgaaaa gatgcgccag tttcgcagtg tctgctttgg tcgttttgac
    15961 ttaattcgta tgtttctgga gatccagatt caggcggcgt ttgctgatgg ttcactgcac
    16021 ccgaatgaac gggcggtgct gtatgtcatt gccgaagagt taggcatctc ccgcgcccag
    16081 tttgaccagt ttttgcgcat gatgcagggc ggtgcacagt ttggcggcgg ttatcagcag
    16141 caatctggca gtggtaactg gcagcaagcg cagcgtggcc cgacgctgga agatgcctgt
    16201 aatgtgctgg gcgtgaagcc gacggatgat gcgaccacca tcaaacgtgc ctaccgtaag
    16261 ctgatgagtg aacaccatcc cgataagttg gtggcgaaag gtttgccgcc tgagatgatg
    16321 gagatggcga agcaaaaagc gcaggaaatt cagcaggcct atgagctgat aaagcagcag
    16381 aaagggttta aataagtttc aacgtgtagg cctgataaga cgcggtaggc cggataagac
    16441 gcgccagcgt cgcatccggc gagcagcatc agacaatcgg cgcacaattg ccggatgtgg
    16501 cgtgaacgcc ttatccggcc taccgaactt cgccagcgtg actattaaaa atccgctggt
    16561 gctttaaacg tcatactatt gccatacgcc ggatgggtaa tcgtcagcat ctctgcatgt
    16621 agcaacaaac gcggtgccat tgctctcgct tctggtgatg cataaaaacg atcgccgaga
    16681 atcggatgac ccagcgccag catatgcaca cgcagctgat gcgaacgccc ggtaatcggt
    16741 tttaacacca ctcttgccgt gttatccgcc gcatattcca ccacttcata ctccgtctgc
    16801 gcaggtttac ctgtttcgta acagactttc tgtttcgggc ggtttggcca gtcgcaaatc
    16861 agcggcagat ccaccagacc ttccgcaggg gatggatgcc cccagacgcg ggccacatac
    16921 tgttttttcg gctcgcgctc gcggaactgg cgttttaact cccgctccgc ggctttggtc
    16981 agcgccacta caatcacgcc gctggtagcc atatccagac gatgcaccga ttctgcctgc
    17041 ggataatcac gctgaatgcg cgtcatcacg ctgtctttgt gctcttccag acgacccggc
    17101 acagacaaca aaccgctcgg cttgttgacc accataatat ggtcatcctg atacaggaca
    17161 accagccagg gttcctgcgg cggattgtag ttttccatcc ccatcttcgg ctccgttact
    17221 gatgcgtcac aacgatcaaa cgcagggcat ccagacgcca gcctgcctga tccaggcttt
    17281 ccattacctg ctgacggttg ctctcaatgg cggtcagttc gtcgtcacga atgttcgggt
    17341 taactgcacg cagcgcttcc agacgagaca gctcggcaga cagtttttcg tcggcttcgt
    17401 tacgcgctgc atcaatcaat gcacgggcag atttctcgat ctgcgcttcc cccagctgaa
    17461 ggatagcgtg aacatcctgc tgcaccgcgt taaccagctt gctgccggtg tgacggttaa
    17521 ccgcgttaag ctgacggtta aaggtttcaa attctacctg cgccgccagg ttgttgccgt
    17581 ttttatccag cagcatacgt accggcgtcg gtggcaggaa gcggttgagc tgcaactgct
    17641 tcggagcctg ggcttcaacc acataaatca gttccaccaa cagcgtacct accggcaacg
    17701 ctttgttttt caacagtgaa atcgtgctgc taccggtatc gccagaaagg atcagatcca
    17761 gaccgttgcg gatcagcgga tgctcccagg taataaactg tgcatcttca cgcgccagcg
    17821 ccacttcacg gtcgaaggtg atggtgatgc catcttccga caggccaggg aagtccggca
    17881 ccagcatatg atcggacggc gtcagcacga tcatgttgtc gccgcgatcg tcctgattga
    17941 taccgataat atcgaacagg ttcatggcga aggcgatcag attggtatcg tcatcctgct
    18001 cttcaatgct ttctgccagt gcctgggctt tttcgccacc gttggaatgg atctccagca
    18061 ggcggtcacg cccctgttcc agttgtgctt tcagcgcttc gtgctgctcg cggcagtttt
    18121 tgattagatc gtcaaagcct tcggtctcat ccggtgaagc cagatagtta atcagatcgt
    18181 tgtatacgct atcgtaaata gtgcgtccgg tcgggcaggt gtgctcgaac gcatccagac
    18241 cttcgtgata ccagcgcacc agcaccgact gagccgtttt ttccagataa ggcacatgga
    18301 tctgaatatc gtgcgcctga ccgatacgat ccagacgccc aatacgctgc tccagcagat
    18361 ccgggttgaa tggcaggtca aacatcacca tgtggctggc gaactggaag ttacgtcctt
    18421 cagaaccgat ttccgagcac agcagtacct gcgcaccggt gtcttcttca gcaaaccagg
    18481 cggcagcgcg gtcacgttcg ataatcgaca tgccttcgtg gaacactgcg gcgcgaatac
    18541 cttcacgttc gcgcagtacc tgctccagtt gcagcgcagt ggccgctttg gcgcagatca
    18601 ccagcacttt ctgagagcga tgactggtca ggtagcccat cagccattca acgcgcggat
    18661 cgaagttcca ccaggtggcg ttatcgcctt caaattcctg ataaatacgc tccggataga
    18721 gcatatcgcg agcgcgatct tccgcacttt tgcgtgcgcc cataatgccg gagactttaa
    18781 tagcggtctg atactgcgtc ggcagtggca gcttaatagt atgcagctcg cgtttcggga
    18841 agcctttcac accgttacgg gtgttacgga acagcacgcg gctggtaccg tggcgatcca
    18901 tcagcatcga aaccagttcc tgacgggcgc tctgggcatc ttcgctgtcg ctgtttgctg
    18961 cttgcaacag cggctcgata tcctgctcgc cgatcatctc accgagcatg ttcagttcgt
    19021 cattgctgag tttgttaccc gccagcagca tggcaacggc gtccgcaacc ggacgataat
    19081 ttttctgctc ttcaacgaat tgcgcaaaat cgtggaaacg gttcgggtcc agcagacgca
    19141 gacgggcgaa gtggctttcc atccccagct gttccggggt tgcggtcagc agcagaacgc
    19201 ccggcacgtg ctctgccagt tgttcaatgg cctgatattc acggctcggc gcatcttcgc
    19261 tccacaccag gtgatgcgct tcatcgacca ccagtagatc ccactcggct tcacagagat
    19321 gttccagacg ctgtttgcta cgacgggcaa aatccagcga gcaaatcacc agctgctcgg
    19381 tatcgaacgg gttgtaagca tcgtgctgag cttcggcata acgctcatca tcaaacagag
    19441 caaagcgcag gttgaaacgg cgcagcattt ctaccagcca ctgatgctgt aaggtttccg
    19501 ggacgataat cagcacacgt tcagcagcgc cagagagcag ttgctgatgc aggatcatcc
    19561 cggcctcaat ggttttccct aaacccactt cgtcagccaa caggacgcgc ggcgcgtggc
    19621 ggcgaccaac atcatgagcg atgttgagct ggtgcgggat caggctggta cgctgaccgc
    19681 gcaggccgct gtacggcata cggaactgtt cgctggaata cttacgcgcg cgataacgca
    19741 gcgcaaagcg gtccatacgg tcaatctgcc cggcaaacag acggtcctgc ggtttgctga
    19801 acaccagttt gctatcaagg aaaacttcac gcagggccac gccggactct tcagtatcaa
    19861 ggcgagtacc gatataggtc agcaagccat tttcttcttt tacttcttcg acttgcatct
    19921 gccagccgtc atggctggta atggtatcac cagggttgaa catcacgcgg gtcacggggg
    19981 aatcactgcg tgcgtacaga cggttttcac cagtagatgg gaaaagtaaa gtgacagttc
    20041 gcgcatccac cgcgacaacg gttccaagtc ccaattcgct ttctgtatcg ctgatccagc
    20101 gttgaccaag tgtaaaaggc atatgtgttc ggctctatat ctttaattgc aggcaataac
    20161 cacccgctac cgtgcttatg aggtagtggt gttattcagg tccaggaatg gaaagggcgc
    20221 tatggtactg gatggcaaag cattcgtcac gcatcaaaat ggtatctggc gaactctttt
    20281 ttttgctcaa aatagcccaa gttgcccggt cataagtgta gcaaaattat cctcaataaa
    20341 agggagtatt ccctccgcta cgggttgtag ctggcgggtc agatagtgtt cgtaatccag
    20401 tggtgaacgt tggtagtcca gcggctccgg gccgttggtg gtccatacgt acttaatggt
    20461 gccgcggttc tgatattgca aggggcgacc gcgcttatgg ttttcttcat cggcaaggcg
    20521 agcggcgcgt acatgaggcg gcacattacg ctgatattcg ctcagcggac ggcgaaggcg
    20581 tttacggtaa accagccgcg catccagttc acccgccatc agtttgtcga tggtttcacg
    20641 cacgtattcc tgatatggct cgttgcggaa gatacgcagg taaagctcct gctgaaactg
    20701 ctgggccagt ggcgtccagt cggtgcgcac ggtttccagc cctttaaaca ccatccgctg
    20761 tttgtcgccc tcctgaatca gtccggcgta gcgctttttg ctgccggtat cggccccgcg
    20821 aatggttggc atcagaaaac ggcagaaatg ggtttcatac tccagttcca gtgcgctggt
    20881 cagccgttgt ttttgcagcg tttccgccca ccaggcgtta acgtgctgca ccagtgcacg
    20941 accgattttc gctgcttctt cttccgaatg tgcacctttc agccagacaa acgttgagtc
    21001 ggtatcgccg tagataacgt catagccctg cgcttcaatc aacgttttgg tttgccgcat
    21061 gatctgatga ccacgcatgg tgatcgacga tgccaggcgt ggatcgaaga aacggcaggc
    21121 ggtggtgccg agcacaccat aaaaggcatt cataatgatc ttcagcgcct gcgaaagcgg
    21181 cttattaccc tgacgtttgg cttcatcgcg cccgtgccag atgttagtca caatctccgg
    21241 caggcaatgt ttctcccgtg agaaccacgc atcgagaaaa ccttcggtac tgtgctctgg
    21301 atcaggctgc gccatgcctt ccaccagccc gacgggatca atcagaaagg tgcggatgat
    21361 cgacgggtac aggcttttgt agtccagcac cagcaccgaa tcataaagcc caggtcgcga
    21421 atccatcacg tagccgccag ggctggcgtg cggcggcact tcgccgagat taggcgcgac
    21481 ataaccagcg cgatgcatac ggggaaaata gagatgacca aacgctgcca ccgaaccgcc
    21541 gtgtcgatcc accggcaggc cgttcaccgt cgcccgttcg agcaaaaacg gcataatttc
    21601 agttttgtgg aagatctgcg tcaccagctc gcaatctttc aggttataag ttgccagcgc
    21661 aggtttatct tcggcgaaac ggcggtcaat ttcgtccatt cgatcccacg ggttatcgat
    21721 agattttcct tcgcctaata actcctgagc gacggtttcc agcgagaatg aagagaaatt
    21781 ccagaacgcg gatttcagcg cctcgatacc gtcgataatt agccgacctt taacctgagc
    21841 aaaaaacaca ccgtttttaa agccgtgctc gcgccactcc agctcactgt tatcacgccc
    21901 cagccgcagc ggaatacggt aacgctcggc atgtttttgc agcattcgca gatcgaactg
    21961 caccacgttc caaccgatga tcacatcagg atcgtagttg gcaaaccagg cgttaagttt
    22021 ttccagtagc agcgggcggc tggcgacgta ttccagttca aaatcgagcg cggaggcgtc
    22081 gccattctct ggccccagca tataaacgat acgctgcccg caaccttcca ggccgatgca
    22141 gtacagctca ccgtggcggg tggtttcaat atcaatagaa acccacttga gcggcggacg
    22201 atagtcggga tgcggtttca gacgggcatt aacgatagcg ccattgcgca tatcaccctc
    22261 gacccacacc ggtgaggtga taaatcgctc catcagatag cgttctggcg ggcgcacatc
    22321 ggcctcgtag acggtaacgc caccttcacg caggcgcttt tcgtaattca tcaactggcg
    22381 atgggcgcga cagtaaaggc catacaccgg ctggcggtga aaatctttta acgccagcgg
    22441 cgtcaggcga aagccttgtt caccctgcaa aatatgctga gcgcggggaa cctgatcggc
    22501 aggaataaac gccacggact cttgcggcgc aaggaaaacc tgcaacggcc cgttgtccgt
    22561 cgccagccag aaggaaactt cggtcccttg cggggtgtcc cgccagtgtc gggttaagat
    22621 aaaacctgcc tgcgccacgc tgaaaatcca ataaaaaacc aggcttgatt atagcctggt
    22681 ttcgtttgat tggctgtggt tttatacagt cattactgcc cgtaatatgc cttcgcgcca
    22741 tgcttacgca gatagtgttt atccagcagc gtttgctgca tatccggtaa ctgcggcgct
    22801 aactgacggc agaatatacc catataagcg acctcttcca gcacgatggc attatgcacc
    22861 gcatcttcgg catttttgcc ccatgcaaat gggccgtgag aatggaccag gacgccgggc
    22921 atttgcgctg catcgatacc ctgtttttcg aaggtttcta cgatgacgtt accggtttcc
    22981 cactcatatt caccgttgat ttctgcgtcg gtcattttgc gggtgcaggg aatgggaccg
    23041 tagaaatagt cggcgtgggt ggtaccggtt gccggaattg actgacccgc ctgcgcccag
    23101 atggtggcgt ggcgcgagtg tgtgtgcaca atgccgccaa tggacgggaa tgcctgatag
    23161 agcagccggt gagttggcgt gtcggaggag ggctttttcg taccttcaac cacttcaccg
    23221 gtttcgatgc tgaccacgac catatcgtca gcggtcatga tgctgtagtc gacgccggaa
    23281 ggtttgatca caaagacgcc gcgcccgcga tcaacggcgc tgacgttgcc ccaggtgagc
    23341 gtgaccaggt tgtgttttgg cagcgccagg ttggcttcta atacctggcg tttgagatct
    23401 tctaacatgt tgactccttc gtgccggatg cgatttgctt atccggccta caaaatcgca
    23461 gcgtgtaggc ctgataagac gcgtcggcgt cgcatcaggc gttacatacc tgatgcggct
    23521 acttagcgac gaaatccgta atacacttcg ttccagcgca gcgcgtcttt aaacgctgga
    23581 aggcgggtat cgttatcaat caccgtgatt tcaatgtcgt gcatctcggc gaactggcgc
    23641 atatcgttga ggttcagcgc atggctgaag acggtatggt gcgcgccacc ggcaaggatc
    23701 cacgcttcgg aagcggtcgg cagatccggt tgcgctttcc acagcgcatt cgccaccggc
    23761 agtttcggca gggagtgcgg tgttttcacc gtgtcgatgc agttaaccag cagacgataa
    23821 cgatcgccga gatcaatcag gctggcgaca atggccggac cagtttgagt attgaagatc
    23881 aggcgagccg ggtcgtcttt accgccaata ccaaggtgct gaacgtcgag gatcggtttc
    23941 tcttccacag cgatcgacgg gcagacttcc agcatatggg agccgagtac caggtcatta
    24001 cctttctcga agtgataggt gtagtcctcc ataaaggagg tgccgccctg cagaccggtt
    24061 gacatcacct tcatgatgcg aagcagggcg gcagttttcc agtcgccttc gcccgcaaag
    24121 ccgtagccct gctgcatcag acgctgtacg gccagaccag gaagctgttt caggccgtgc
    24181 aaatcttcaa aggtagtagt gaaagcgtgg aagccaccct gttccaggaa acgcttcatc
    24241 cccagctcaa tacgcgccgc ttccagcacg ttctgtcgtt tttcgccgtg gatttgtgtg
    24301 gcaggtgtca tggtgtagca gctttcgtac tcatcgacca gcgcgttaac atcgccgtcg
    24361 ctgatggagt tcaccacctg caccagatcg ccaaccgccc aggtattgac ggagaaacca
    24421 aacttgatct gtgcggcaac tttatcgcca tcggtgaccg ctacttcacg catgttatcg
    24481 ccaaaacggc agactttcag atgacgggta tcctgtttag agaccgcctg acgcatccag
    24541 gagccgatac gctcatgtgc ttgtttatcc tgccagtgac cggtaacaac ggcatgttgc
    24601 tgacgcatac gcgcgccgat gaagccgaac tcgcgaccgc catgcgcggt ctggttcagg
    24661 ttcataaagt ccatgtcgat gctgtcccac ggcagcgccg cgttgaactg ggtgtggaat
    24721 tgcagcaacg gtttgttgag catggtcagg ccgttgatcc acattttggc cggggagaaa
    24781 gtgtgcagcc acaccaccag accagcgcaa cgatcgtcgt aattcgcgtc gcggcaaata
    24841 gcggtgattt catccggcgt ggtgcccagc ggtttcagca ccagtttgca gggcaacttc
    24901 gcttctgtat tcagtgcatt aacaacgtgt tccgcatgtt gcgtcacctg gcgcagagtc
    24961 tccgggccgt aaagatgctg gctgccaatt acaaaccaca cttcataatt atcaaaaatc
    25021 gtcattgtcg tgtccttata gagtcggaac ggcctgggca gcctgtgccg gggcggaagt
    25081 tggaagatag tgttgttcgg cgctcatcgc ccattgctga tagcggcgat aaagctgttc
    25141 aaagcgttgt gcctgttcgc tgcgcggttg cagggttttc tctaccgcac tggccatttt
    25201 ttgctgggct gatgggatgt ctgcgtgcac tttcgcggcg acagcagcaa aaatcgccgc
    25261 accgagcgca cagcactggt cagaggcaac aatttgcagc gggcgattca gcacgtcgca
    25321 gcaggcctgc ataatgacct ggtttttccg cgcgatgccg cccagcgcca tcacgttatt
    25381 gacggcgatc ccctgatcgg taaagcactc catgattgcg cgtgcgccaa aggcggtggc
    25441 agcaatcaaa ccgccgaaca gcagcggagc gtcggtagcg aggttcagat cggtaatcac
    25501 ccctttcagg cgttggttag cgttcggtgt gcggcggccg ttaaaccagt cgagcaccac
    25561 cggtaggtga tccagagacg gattttgagc ccatacttcg gtcagcgccg gaagcagttg
    25621 tttctggctg gcgttgattt gctctttcag ttccggatgc tgggcggcaa gctgttccag
    25681 cggccagccg agtacgcgac caaaccaggc gtagatatcc ccaaacgccg attggcctgc
    25741 ttccagaccg ataaatccag gcaccacgct gccatcaacc tgaccgcaaa tacctttcac
    25801 tgcccgctcg ccaacgctct gtttgtcggc aatcagaatg tcacaggtgg aagtaccgat
    25861 aacttttacc agtgcgttag gctgtgcgcc tgcgccaact gcgcccatat ggcagtcaaa
    25921 cgcgccgccg gaaatcacta cgctttcagg caggccgaga cgctgcgccc attccgggca
    25981 taaggtgccc accggaatat cggcagtcca ggtatcagtg aacagcgggg aaggcaaatg
    26041 gcgattgagg atcgggtcca gctcatcaaa gaaactggct ggcggcaggc caccccagct
    26101 ttcgtgccac agagatttat gcccggcgct gcaacgtccg cgacgaatat cctgcgggcg
    26161 ggtggtaccg gaaagcagag ctggcaccca gtcgcacagc tcaatccatg atgcggcaga
    26221 ttgtgccacg gcgttgtcct ggcgagttac atgcaggatt tttgcccaga accattcgct
    26281 ggaataaata ccgccaatat tgcgggagta gtcaacgttg cccggcgtgt gacataagcg
    26341 ggtaatctct tccgcttctt caaccgcggt gtggtctttc cacaatacga acatcgcgtt
    26401 cgggttctcg gcaaactccg ggcgcagcgc caacacgttg ccgtcggcgt caatcggtgc
    26461 gggcgtcgag ccggttgtgt caacgccaat cccgaccaca gctgcgcgct gttcgacgca
    26521 aagctctgca agcacagttt tcagcgccgc ttccattgac tcaatgtagt cacgcggatg
    26581 atgacggaac tggttattcg gggcatcaca aaattgcccg ttctgccaac ggggatacca
    26641 ctctacgctg gtggcgatct cttcaccagt ggcgcattcc accgccaaag ctcgcacaga
    26701 atcactgcca aaatcgaggc caattgcaat cgccatcgtt tcactccatc caaaaaaacg
    26761 ggtatggaga aacagtagag agttgcgaaa aaaaacgtca ggctggatcc gctaatctta
    26821 tggataaaaa tgctatggca tagcaaagtg tgacgccgtg caaataatca atgtggactt
    26881 ttctgccgtg attatagaca cttttattac gcgtttttgt catggctttg gtcccgcttt
    26941 gttacagaat gcttttaata agcggggtta ccggttgggt cagcgagaag agccagtaaa
    27001 agacgcagtg acggcaatgt ctgatgcaat atggacaatt ggtttcttct ctgaatggcg
    27061 ggagtatgaa aagtatggct gaagcgcaaa atgatcccct gctgccggga tactcgttta
    27121 acgcccatct ggtggcgggt ttaacgccga ttgaggccaa cggttatctc gattttttta
    27181 tcgaccgacc gctgggaatg aaaggttata ttctcaatct caccattcgc ggtcagggca
    27241 tagtgaaaaa ccagggacga gaatttgtct gccgaccggg tgatattttg ctgttcccgc
    27301 caggagagat tcatcactac ggtcgtcatc cggaggctca cgaatggtat caccagtggg
    27361 tttactttcg tccgcgcgcc tactggcatg aatgggttaa ctggccgtcg atatttgcca
    27421 ataccggttt ctttcgcccg gatgaagcgc accagccgca tttcagcgac ctgtttgggc
    27481 aaatcattaa tgccggacaa ggggaagggc gctattcgga attgctggcg ataaatctgc
    27541 ttgagcaatt gttactgcgg cgcatggaag cgattaacga gtcgctccat ccgccgatgg
    27601 ataatcgggt acgcgaggct tgtcagtaca tcagcgatca cctggcagac agtaattttg
    27661 atatcgccag cgtcgcacag catgtctgcc tgtcgccgtc gcgtctgtcg catttgttcc
    27721 gccagcagtt agggattagc gtattaagct ggcgcgagga ccagcgcatc agtcaggcga
    27781 agctgctttt gagcaccacc cggatgccta tcgccacagt cggtcgcaat gtcggttttg
    27841 acgatcaact ctatttctcg cgagtattta aaaaatgcac cggggccagc ccgagcgagt
    27901 ttagggctgg atgtgaataa taagaaagaa gggggagtct
//
LOCUS       DACADF010000056        27835 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.056, whole genome shotgun sequence.
ACCESSION   DACADF010000056 DACADF010000000
VERSION     DACADF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.056"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(80..1048)
                     /gene="astE"
                     /locus_tag="ISB30_002239"
     CDS             complement(80..1048)
                     /gene="astE"
                     /locus_tag="ISB30_002239"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAP1989869.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPRFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1041..2384)
                     /gene="astB"
                     /locus_tag="ISB30_002240"
     CDS             complement(1041..2384)
                     /gene="astB"
                     /locus_tag="ISB30_002240"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAP1989870.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKKLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEKKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVSGFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWCY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            complement(2381..3859)
                     /gene="astD"
                     /locus_tag="ISB30_002241"
     CDS             complement(2381..3859)
                     /gene="astD"
                     /locus_tag="ISB30_002241"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HAP1989871.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSFSDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLLPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRPLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(3856..4890)
                     /gene="astA"
                     /locus_tag="ISB30_002242"
     CDS             complement(3856..4890)
                     /gene="astA"
                     /locus_tag="ISB30_002242"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAP1989872.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSESGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(4887..6107)
                     /gene="astC"
                     /locus_tag="ISB30_002243"
     CDS             complement(4887..6107)
                     /gene="astC"
                     /locus_tag="ISB30_002243"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HAP1989873.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWEQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQLSQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLER
                     FAAACEHFVSRGSS"
     gene            6553..7359
                     /gene="xthA"
                     /locus_tag="ISB30_002244"
     CDS             6553..7359
                     /gene="xthA"
                     /locus_tag="ISB30_002244"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP1989874.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPISVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     ITVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            7526..8236
                     /locus_tag="ISB30_002245"
     CDS             7526..8236
                     /locus_tag="ISB30_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP1989875.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            8241..8918
                     /gene="ydjY"
                     /locus_tag="ISB30_002246"
     CDS             8241..8918
                     /gene="ydjY"
                     /locus_tag="ISB30_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAP1989876.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            8932..9639
                     /locus_tag="ISB30_002247"
     CDS             8932..9639
                     /locus_tag="ISB30_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP1989877.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            9639..10187
                     /locus_tag="ISB30_002248"
     CDS             9639..10187
                     /locus_tag="ISB30_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP1989878.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10197..11363
                     /locus_tag="ISB30_002249"
     CDS             10197..11363
                     /locus_tag="ISB30_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP1989879.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEVAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTLQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            11336..12871
                     /locus_tag="ISB30_002250"
     CDS             11336..12871
                     /locus_tag="ISB30_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1989880.1"
                     /translation="MATPLRYALIFLLWAMIAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPSNTLASFLPLTGVLCVVLLAILADQSTINSQALINSLTMGLVAAFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVVKWVGYVRQ
                     GLR"
     gene            12871..13524
                     /locus_tag="ISB30_002251"
     CDS             12871..13524
                     /locus_tag="ISB30_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP1989881.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPVLDMAQWSENYNKLR"
     gene            13591..14898
                     /locus_tag="ISB30_002252"
     CDS             13591..14898
                     /locus_tag="ISB30_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAP1989882.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(14907..15527)
                     /locus_tag="ISB30_002253"
     CDS             complement(14907..15527)
                     /locus_tag="ISB30_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP1989883.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            15614..16021
                     /gene="nudG"
                     /locus_tag="ISB30_002254"
     CDS             15614..16021
                     /gene="nudG"
                     /locus_tag="ISB30_002254"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAP1989884.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKIEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALRYPLAPADIPLLEAFMASRAARPAD"
     gene            complement(15987..16259)
                     /locus_tag="ISB30_002255"
     CDS             complement(15987..16259)
                     /locus_tag="ISB30_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAP1989885.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            16495..17838
                     /gene="gdhA"
                     /locus_tag="ISB30_002256"
     CDS             16495..17838
                     /gene="gdhA"
                     /locus_tag="ISB30_002256"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP1989886.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(17915..19870)
                     /locus_tag="ISB30_002257"
     CDS             complement(17915..19870)
                     /locus_tag="ISB30_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP1989887.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETYKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPPSEEAGNGVII"
     gene            complement(19875..20918)
                     /gene="selD"
                     /locus_tag="ISB30_002258"
     CDS             complement(19875..20918)
                     /gene="selD"
                     /locus_tag="ISB30_002258"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAP1989888.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGVSFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(21035..21586)
                     /locus_tag="ISB30_002259"
     CDS             complement(21035..21586)
                     /locus_tag="ISB30_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAP1989889.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            21747..23603
                     /gene="sppA"
                     /locus_tag="ISB30_002260"
     CDS             21747..23603
                     /gene="sppA"
                     /locus_tag="ISB30_002260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP1989890.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEVQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23719..24429
                     /locus_tag="ISB30_002261"
     CDS             23719..24429
                     /locus_tag="ISB30_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HAP1989891.1"
                     /translation="MNKAPSLIAAIVLGLGISACGYFVGDGVKHLKTNNRYVNVRGLS
                     EKEVRADTAELTIAINFKGNIPGELFPKLEDAQKKIVAELNAQGINEKEITLGQWTSK
                     RTDSFYLKDDPTMPRYNADGSVTIKTHNVAAVEKVVAKLNELQVATEGAIAESKVAYR
                     FNGIGALRAEMIAAATKDARNAALQFATDSGSQVGSISDASQGVFQIFASGSDEDDPT
                     AINKTVRVVTTVTYALQD"
     gene            24561..25577
                     /gene="ansA"
                     /locus_tag="ISB30_002262"
     CDS             24561..25577
                     /gene="ansA"
                     /locus_tag="ISB30_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP1989892.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLSKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQKLDTETIRKAMSQNLRGEL
                     TPDD"
     gene            25588..26229
                     /gene="pncA"
                     /locus_tag="ISB30_002263"
     CDS             25588..26229
                     /gene="pncA"
                     /locus_tag="ISB30_002263"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAP1989893.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(26322..27680)
                     /locus_tag="ISB30_002264"
     CDS             complement(26322..27680)
                     /locus_tag="ISB30_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1989894.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGRIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWMPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
BASE COUNT         6677 a         7504 c         7095 g         6559 t
ORIGIN      
        1 agcaaataac caggattaat ctgtattaat ttataagaaa gcaacttaat acccgcagaa
       61 tgatttctgc gggtaagtat tagcttattt tttcgagcat taatcccgca cgtaatccca
      121 acgctaccaa cggattaggg aataacacat actctacatc atgggttacg gtaaaacgtt
      181 cctccccgtc ctgcgccagc aatgttcctt tctcaaacgg cataaaattc agcgtgtcat
      241 ttgccatatg catttcgaag gacggcgagt ggcgagtaat ttgcgaaacc acccgataac
      301 ggagcggcgg tgttctcacg ataccgacac tctcaccaga tagcagcgca gcaattgcgc
      361 tggcagttac tgcaaactgg cgaagatcgt tttgtccaaa gggcaacgct ttgccgagtt
      421 ccagcgtaca ggccagcgcg ccaaaatgtc tggcgctgaa atgggtaaac gtaccaccag
      481 gctcctgatg gaacaccagt gcctccagac ccgccgcacc cagccacgtc agaaatttct
      541 cgtcccaggg aatgtcgcgt tgcggtaata caccgaaccg cggatgcaag gagccacgaa
      601 ttgcggtatg gagatcaagg tgccagcgca cagattcttt gccctggtca taaaaatctt
      661 ccaggcactg ttccagttcg cgcgcccgac aggtttcccc gctttcagca aatagctgcc
      721 aacgaccgcc aaacattcgg ttcatatcgc tatggcaata acgtttccct tgcttcagcg
      781 caggaggatt cccgaggatc accagcaacc gccaacgtaa cgggatctcg ccgtgagata
      841 tcgcgccaag caacgcatcc agcatttcca caggtgccgt ctcattaccg tgtattcccg
      901 ctgaaatcac cagtgcgcct tgcggtggcg ttaatggcgt cagttccagc acgccatcgc
      961 ccagccagcg ccagcgaacg ccgttgattt ctcgctcggt gataaccggt tttttacccg
     1021 ttaaggtcag agcaagaaaa ttatccattt ccccctccct cgcgctggaa tggataaacc
     1081 gaaccaagat ccaataattg agtcagtgta tccagcgctt cccgtccttc gcgcagcaat
     1141 tgtggatcgg caagatccgc cgcagtaaga cgatcgcggt agtaacgatc cacccagtca
     1201 ttgagcgcat taaacagcgt atcgttcatc atcaccgccg gattcaccgc ccggcgttct
     1261 tcttctgtca acaccacgcg cagccgcagg cacgccgggc caccgccatt cgccatgctt
     1321 tcacgtaaat caaaaacttt tagtgagctt attgggttat cggcagcaag caattcattg
     1381 agataacacc atactccagc atgttcccga cattcctgag gcagcaccaa catcatggaa
     1441 ccatcatcgc ggctcagcag ttggctgtta aacagataag tagacaccgc atctgacacg
     1501 gaaacctgcg ccgtgggcac ctctattgcc ataaaaccac tgacccgtgc acgcagattt
     1561 gccagtaact gtgactggcg agcgaacgcc tgttgatggc aaaacagcac ctggcggtta
     1621 ctcacggcaa tcacgtcatt atgaaaaacg ccctggtcga taacgtccgg gttttgctgg
     1681 gcgaaaatca cctgttgggg attcacctga ttcaaccttg ccaccgcctc gctggcttca
     1741 cgggtctgcc gcgccggata acgggaaggc cgggtatcat tgccctcttc tcgcccgtag
     1801 acaaaaagtt gcatacccgg ttcaccgtaa tgaccgccga gacgattgtg gtttgccgcc
     1861 ccctcatcac cgagcaaggc tacctgcggc aacgccgaat ggacgctaaa tttcttttcg
     1921 tcgttaaaaa tcgcttttaa cagcgattcg gtgacggggg cttccagcga acggtgaaat
     1981 ttattgttca ggttagcaac agtgagatgc actttgccat ccagcgtatc ggcggatggc
     2041 gcgatcgttg ccgcattggc tacccacatt ggcgaagcgg aactgacgct ggaaagccag
     2101 tgcggtgcct ggcgtgcaac tttttccagt acctgctcat cgctaccact gaatcccaac
     2161 tgacgcagca ccggaataaa cggacgctcg tgcggcggga tcacggcctg ggggaatccc
     2221 gcatcggcaa gttttttcat tttcagtaag ccctgttttg ccgccagtcg cgggttagac
     2281 acctgaaaac ggtgacgggt agaggcttca ttaccaaacg acaggcccgc gtaatggtgc
     2341 gtcagcccta ccagcccgtc gaaattgact tcccaggcgt tcatcgcacc acctcatcgg
     2401 aaaaatccag cccggggtta agcgttgcgg gcaacgttaa cgagtccgac tccagacttg
     2461 ccatcggcca ggcacaataa tctgcggcat accaggcgct ggggcgatga ttgccggaag
     2521 cgccaatgcc gccgaatggc gcggtactgg cagcaccggt aagcggtttg ttccagttaa
     2581 caatccccgc ccgtgcctcc agcaacagtt gatcgaattt ttcccgctcg ggggaaacca
     2641 gaccgcaaga gaggccgaag cgagtgttat tcgccattcg aatcgcttca tcgaaattgt
     2701 cataacgcca gacgcgcaat aacggcccaa acacttcttc atccggcacg ccagtaacgc
     2761 ctgtcatttc aatgatcccc ggcgtcagca acgatgtccc tgcttgtaat aagcgcggcg
     2821 caagcagggg ccgtccgccc atcgcctcca gttcctgcca ggcagtaacc acctgctgtg
     2881 cggcctgctc agaaatcagc ccgccaataa acggctgcgg ttcgtcatcc caagtgcccg
     2941 gcgttaaccg ctggctgacg gcaaccaggc gagcaagaaa tgcatcgccc tgcgttccac
     3001 ttttcagcaa taaacggcgg gcgcaggtgc agcgttgccc ggcagtgaca aacgccgact
     3061 gaatggtcag atggacagcg gcatcgatat ccgccacctc atcgataatt agcgggttat
     3121 taccgcccat ctcaagggcg agaattttct ccggctgacc ggaaagctgg cgatgcagct
     3181 ggtagccggt attggcgctg ccggtaaaca gcaaaccgtc gagatcctcc agcgcactca
     3241 gcgcctgacc cgtttcacgc ccgccctgca ccaggttcag cacgcccggc agcaagccag
     3301 cctgctgcca taaacgcatt accgcttcgc cactccacgg tgtcagttcg ctgggtttaa
     3361 aaataatggt gttacctgcc agcaatgccg gaacaatatg tccgttcggc agatgaccag
     3421 ggaaattata cggtccaaac actgccagta cgccgtgcgg acgatggcgc aagctcgccg
     3481 cgccgtccgg catttcgcta cgctgctcac cggtacgaac gtgatacgcc ttaattgata
     3541 tcgcgatttt attgatcatc gccgtcactt cggttgccgc ttcccagcgc ggcttgccgg
     3601 tttctctggc aataatcgcg gttaattcag ctttattgct ttccagcaat ccggcaaagc
     3661 gttcaactct tacctgacga tcgctaaatg agagcctcgc ccagcgcgga aacgccgctc
     3721 gggcagcccg acaagcctgc tcgacctgag cagcatcggc atcattgccc tgccataaca
     3781 cctcgcccga taccggatta cgcttcacac gcaatgcgcc ctggcccgtt acccagtcac
     3841 cgttaatcca taaagtcatg ctgttttctc ctctgcgcac aggcgcacca gacgaacgcg
     3901 atcccctgcg tggcatttga gggcatccag ttgtgcggcg gttaaaatca aacgctcggt
     3961 cgctggatcg gtacgcacca gcaccacgcg gaaatggtga taattttcat tggcgaccag
     4021 gcaggccgga aaatcgccct gcgcaggctg cccttcggcc acttccacca gccgactttt
     4081 acggatggcg cgcacgcggt cgatatcaca ctcaagtgtc ggcccgccgt caaagatgtc
     4141 gatatagtta cggtagcgaa aaccttcttt ctccagcacc gcacgggcag gtgcggtttg
     4201 cggatgtacc tgaccgatga cgtcctgtgc ttcctgggat aaaaagtgcg tatagatcgg
     4261 atgtttcggc atcagttctg caataaatgc cttttgcccg gtgccgcaga gaaaatcagc
     4321 gcggctaaaa tccatcgaaa agaagcgttt accgaggctt tgccagaacg gtgaatagcc
     4381 gtgttcgtca atcaccccgc gcatttcggc gaccactttg tcattaaact tgtcgcgaaa
     4441 agccgccata aacataaagc gcgatttcga cagcaaatag ccgttgccct ctttgcgcca
     4501 ctccgggtcg agaaacagcg tgcacagctc gctgctgccg gtgtgatcgt tactgagaaa
     4561 cagcgtcggc aatgcgttat agacattcag ctctttcgaa gcgtgaacca gcgtgccgac
     4621 gcgatagttg taccagggat cgttcagccc aaccgccacc tcaatggcgc aaatccccgc
     4681 cacagtgcct gactcgctat cttccagtac aaacacataa ccctgctcac ttttgggcag
     4741 ttcgccttgc caggttttga ttgccctttc aatacgcacc gaaagcgtgg cttcattggc
     4801 gggaagcgac gtcaggccgc cgcccgtttt gctggcaagc tgcatcagcg ccgagacatc
     4861 tgagcgctca acgggacgaa tgaccatcat gatgaacctc ggctaacaaa gtgttcgcaa
     4921 gcagctgcaa agcgctccag tccggtcgtc acctcttctt cgctgacatt gagcgctggc
     4981 gcaaaacgca ccacgttgcc acccgcaatc agcaccatca cgcctgcttt cgccgcttcc
     5041 tgagagagct gtttcgcttg cccggcgtaa tcggcattca gtacacagcc aatcaaaaga
     5101 cctaagccgc gaacttcact gaacaaactg tagtggtgat taatggaatt tagacgctca
     5161 acaaaccagt cgtgacgctg tttaaccccg ttaagcatct ctggcgtgtt gatgagatcc
     5221 agcactttgc ctgccaccgc cgaggccagc ggattgccgc cataggtggt gccgtgagtg
     5281 ccaacggtca tcacgctggc gcactcttcg gttgccaaca acgcaccaac cgggaaaccg
     5341 ccgcccagcg ctttggcggt agttaacaga tcaggcgtca cgccgtagtg catataggca
     5401 tacagttccc cggtgcgccc gacaccggtt tgtacttcat caaagataag caacgcattg
     5461 tggcggtcac acaattcacg cagaccttgt aaaaatgcgt tgctggctgg caccacaccg
     5521 ccttccccct ggatgggttc gacaatcacc gcacaggtgg cgtcgtcaat cagcgcactg
     5581 gcagagttaa tatcgttata tgcagcatga cgaatatccg gcggcagtgg cgcaaaatcc
     5641 tgtgaatagg ctggctgccc acccgcactg acagtaaaca gcgtgcgacc atgaaacgca
     5701 tttttgaacg ccacgatgcc gctcttatgg ctgccgtagc ggtcgtgagc gaatttacgc
     5761 gccagtttta gcgccgcttc gttggcttcc gcaccggagt tacaaaagaa gacgcgatcg
     5821 gcaaacgtgg cgtcgatcaa ttttttcgcc agtcgcagta ccggctcgtt ggtgtaaccg
     5881 ttgccggtat gccagaactt actcgcctgt tcgttcagcg cctcacgcag ttccggatgc
     5941 gcatggccca gcgcgttcac cgcaatgcca cccgcgaagt cgatatactc tttcccctgc
     6001 tgctcccaca agcgcgaacc ttcgccacgt accggtataa acggtgccgg agcgtaaaca
     6061 ggtatcatcc attcatcaaa gttttcacgc gtaattggct gagacatagc gacctctaca
     6121 gtaaataatt agttatttat atgttaataa taagtaatgt ttgcgctgta aatgtagatt
     6181 gcagggttcg tgccagccag tgataaaagt gcataaacgg cggaggctaa ctggaaatca
     6241 agcagttata accacatcat atgcatttaa agtgcatata aagtgaatac gtttgcgatg
     6301 tgggtgaata aaaagaataa aaaacgcaat gttatgcaga agtaaaatat aattctggaa
     6361 ttgtgatcat tgacgaaatt tactggaaat tactgcgcca ttctgacgca acgcgcacca
     6421 aaagcgggca ttttttgcgc catcgttgac atcattaaca accatcgatc aaatcactta
     6481 acaacaggcg gtaagcaaag cgaaattctg ctaccatcca cgcactcttt atctgaataa
     6541 atggcagcga ctatgaaatt tgtctctttt aatatcaacg gcctgcgcgc cagacctcac
     6601 cagcttgaag ccatcgtcga aaagcaccaa ccggatgtga ttggcctgca ggagacaaaa
     6661 gttcatgacg atatgtttcc gctcgaagag gtggcgaagc tcggctacaa cgtgttttat
     6721 cacgggcaga aaggccatta tggcgtggcg ctgctgacca aagagacgcc gatttccgtg
     6781 cgtcgcggct ttcccggtga cgacgaagag gcgcaacggc ggattattat ggcggaaatc
     6841 ccctcgccgc tgggcaatat caccgtgatc aacggttact tcccgcaggg tgaaagccgc
     6901 gaccatccga taaaattccc ggcaaaggcg cagttttatc agaatctgca aaactacctg
     6961 gaaaccgaac tcaagcgtga aaatccggtg ctgattatgg gcgatatgaa tatcagtcct
     7021 ggcgacctgg atattggcat tggcgaagag aaccgtaagc gctggctgcg taccggaaaa
     7081 tgttctttcc ttccggagga acgcgaatgg atggagaggc tgatgagctg ggggttggtc
     7141 gataccttcc gccatgcgaa tccgcaaaca gcagatcgtt tctcatggtt tgattaccgc
     7201 tcaaaaggtt ttgacgataa tcgcggtctg cgcatcgacc tgctgctcgc cagccaaccg
     7261 ctggcagaat gttgcgtaga aaccggcatc gactatgaaa ttcgcagcat ggaaaaaccg
     7321 tccgatcacg cccccgtctg ggcgaccttc cgccgctaat ttagctgatc tcctggctca
     7381 aactgggtca ggagaattaa ccttgagaaa aatcaacaaa ctgtcagtaa tgatttgttg
     7441 cccgccgtcc tttgttatac cgtcactgcg tttttagttg tctgaccact tctctattat
     7501 caagtttgat ataggaaact ccacgatgaa cgctgagcgt aaatttcttt ttgcctgtct
     7561 tatttttgcg ctggtcattt acgctatcca cgctttcggt ttattcgatc tgctcaccga
     7621 tttaccccac ttacagacac tcattcgcca gagcggattg ttcggctata gcctctatat
     7681 tctgttattc atcattgcca ccctctttct gttaccagga agcatactgg tgatcgcggg
     7741 aggaatagtt tttggcccgc tcttagggac actactatca ttaattgccg ccacgctggc
     7801 ctcctcgtgc tcattccttc tggcgcgctg gctgggacgt gatttactgc tgaaatacgt
     7861 tggccatagc catacctttc aggccattga aaaaggcatt gcgcgtaacg gtattgattt
     7921 tcttattctg acccgcttaa tcccgttgtt tccttacaat attcaaaatt acgcttacgg
     7981 attaaccaca atcgccttct ggccttatac ccttatttcg gcactcacga ccctacccgg
     8041 tattgttatt tataccgtga tggcaagcga tctcgccaat gaaggcatta cgctgcgctt
     8101 tattttacaa ctctgtctgg cgggcctggc actgtttatt ctcgtccagc tcgcaaaact
     8161 ctacgcccga cacaaacatg tggatctgtc tgcttctcgc cgcagcccac tttctcaccc
     8221 aaaaaatgaa ggatagaacg atgttgcaac attattcagt gtcatggaaa aaaggactgg
     8281 ctgcactctg cttactggct gttgctgggc ttagcggctg cgatcaaaaa gagaatgcgg
     8341 ctgcaaaagt ggaatacgac ggactttcga acagccaacc gctgcgtgtc gatgccaata
     8401 accatacggt aaccatgctg gtgcaaatta atggtcgttt cctcaccgac gacactcgtc
     8461 acggtattgt gtttaaagat ggctccaacg gacataaatc gctgtttatg ggttatgcga
     8521 ccccgaaagc attttatgaa gccctgaaag aggcaggtgg tacgccgggc gaaaacatga
     8581 caatggataa taaagaaacg actcatgtca caggcagcaa actggatatt tcggtcaact
     8641 ggcaaggggc ggcaaaagcg tattccttcg atgaagtgat tgttgacagt aatggcaaga
     8701 aactggacat gcgctttggc ggtaatttaa cggcagcaga agagaagaaa acgggttgcc
     8761 tggtgtgtct ggatagctgc ccggtcggca tcgtcagcaa tgcaacatac acttatggtg
     8821 cggtggaaaa acgcggcgaa gtgaaattca aaggcaatgc ctcagttctc ccggcggata
     8881 acacgctggc aacggttacc tttaaaatca ccgaataaag caggataaag gatgatgaag
     8941 atgcaatcgc gtaaaatctg gtactaccgt ataaccctca tcatcctgtt gttcgctgtg
     9001 ctgctggcat gggcgctgct tcccggcgtc catgagttta tcaatcgcag cgttgcggcg
     9061 tttgccgccg tggaccaaca gggtatagaa cgctttattc agtcttacgg cgcactagcg
     9121 gcagttgtct cgttcttgtt gatgatttta caggccattg ctgcaccgct acctgcgttt
     9181 ttgatcacct ttgccaatgc gtcgctgttt ggcgcgttct ggggcggctt actgtcgtgg
     9241 accagttcga tggccggcgc ggcgctgtgc ttttttatcg ccagagtgat gggccgcgaa
     9301 gtggtggaaa aattaaccgg caaaaccgta cttgacagta tggacggctt tttcactcgc
     9361 tacggcaaac acaccatcct ggtatgtcgg ttattgcctt ttgtcccttt cgatccaatc
     9421 agctatgctg ccggtttgac ttcaatacgt tttcgctcgt tttttatcgc caccgggctt
     9481 ggtcaattac cggcgactat tgtttattcc tgggcgggca gcatgttaac aggcggtact
     9541 ttctggtttg tcaccggact gtttattctg tttgccctga ccgtggtgat ttttatggcg
     9601 aagaaaatat ggcttgagcg ccagaagagg aatgcctgat gggtttaccg ccgcttagca
     9661 aaattccttt cattttacgt ccgcaggcgt ggctgcatcg tcgccattac ggcgaggtgc
     9721 taagcccgat tcgctggtgg gggcggatcc cgtttatctt ttatctggtg tcgatgtttg
     9781 tcggctggct ggagcgcaaa cgctcaccgc tcgatccggt agtacgatcg cttgtcagcg
     9841 cgcgcattgc gcaaatgtgc ctgtgtgagt tttgcgtgga tatcaccagt atgaaagtcg
     9901 ccgagcgtac cggcagcacc gataaactgc tggcagtggc tgactggcgg caaagcccgc
     9961 tctttagcga tgaagaacgg ctggcgctgg agtacgccga agccgcaagc gtaacgccgc
    10021 caacggtcga tgatgctctg cgcgcccgac tggctacgca ttttgacgct caggcgctca
    10081 ccgaactgac ggcattgata ggcctgcaaa atctgtcagc ccgttttaat tctgccatgg
    10141 acattcccgc tcaggggctg tgccgtattc ctgaaaaacg ttcttaagga gagaagatgc
    10201 gccattgtgg gtggttgctg ggattgttat cgctgttttc tttggcaaca catgccagtg
    10261 actggcagga aataaaaaat gaggccaaag gacaaaccgt ctggtttaac gcctggggcg
    10321 gcgataccgc aattaaccgc tatctcgact gggttagcgg cgagatgaaa acccattacg
    10381 ctataaacct gaagattgtt cgtctcgcgg atgccgcaga cgcggtgaaa cgcattcaga
    10441 ccgaagttgc tgccggacgt aaaacgggcg gttcggtgga tctgctctgg gtgaacggcg
    10501 aaaacttccg caccttaaaa gaggccaatt tattacaaac cggctgggcg gagactctgc
    10561 ccaactggcg ctatgtcgac acacagcttc cggtgcggga agatttttca gtgcctacag
    10621 aaggggctga atcgccctgg ggcggcgcac aactgacgtt tatcgcccgc cgcgatgtta
    10681 cgctacagcc accacaaacg ccgcaagcct tactggagtt tgctaaagcc aatcccggca
    10741 cggttaccta tccgcgccca ccggacttta ccggcacggc gtttcttgaa cagttgctga
    10801 ttatgctaac gccagatccc acagcattaa aagaagcgcc ggacgatgcc actttcgccc
    10861 gcgtcactgc gcccttgtgg caatatcttg atgcactgca tccgtatttg tggcgcgaag
    10921 gaaaggattt cccgccttcg cccgcgcgga tggatgctct gctgaaagct aacacattgc
    10981 gcctgtcgct gacctttaac cccgcgcatg cgcagcaaaa aatcgccagc ggcgatttgc
    11041 ctgcaagcag ttacagtttt ggttttcgcg aggggatgat tggcaacgtc catttcgtca
    11101 ccattcctgc caacgcgaat gccagtgctg cggcgaaggt agttgccaat ttcctgctct
    11161 cacccgatgc gcaactgcgt aaagcagatc ctgctgtctg gggcgatcct tctgttctcg
    11221 acccgcaaaa actgcctgac gggcagcgcg aaacattgca atcgagaatg ccgcaggatc
    11281 tgccgccggt actggctgaa ccgcacgcag gttgggtaaa tgcgctggaa caagaatggc
    11341 tacaccgtta cggtacgcat taatcttttt gctgtgggcg atgatagcgg tgatttatgc
    11401 accgctgatc ccggcagctc tcacgctgat atcgcctgct ttgtcgttga cgcactggca
    11461 ggcgttattt gccgatccac agttaccgca ggcattactg gcgacgctgg tgtcgacaac
    11521 cattgcggcg gtcggggcgt tgttgattgc cctgctggtg attgtggcgt tgtggccagg
    11581 gccgaaatgg cagcgtatgt gcgcccgtct gccgtggctg ctcgccattc cccatgtggc
    11641 ttttgccacc agcgcccttc tgctctttgc tgacggaggg ctgctttatg actatttccc
    11701 gtatttcact ccgccaatgg acaaattggg tattgggctg ggcctcaccc tggcggtgaa
    11761 agaaagcgca tttctgctgt ggatcttagc ggcagtattg agcgaaaaac ggctgttgca
    11821 gcaactcatt gtgctggatt cgctgggcta cagccgctgg caatgcctga actggctgct
    11881 gttgccctcc gtcgcgtctg cgttagcaat ggcgatgctg gcgattgtcg cctggtcgct
    11941 gtcggtcgtg gatgtggcga ttattcttgg gccaggtaat cccccgacgc tggcggtaat
    12001 tagctggcag tggttaactc agggcgacgc cgatcaacaa acgaaaggcg cactcgccag
    12061 cctgctgctg atgctgttac tcgccgccta cgttttgctg ggctatctgc tatggcgtgg
    12121 ctggcggcgt actattcccc gcgtagatgg cgttcgtaag cctgccacgc ctttattgcc
    12181 aagcaatacg ctggcgagtt ttttaccctt aaccggtgtg ctgtgtgtgg ttctgctggc
    12241 gatcctcgcg gatcagtcga cgatcaatag tcaagcgctc atcaacagcc tgacaatggg
    12301 gctggtggcg gcattcatcg ctttgctctt gttactgctg tggctggaat gggggccaca
    12361 gcgtcgccag ttgtggctat ggttgcccat tttattaccc gctctgccgc tggtggcggg
    12421 ccagtacacg ctggcgctat ggctgaacct ggatggaagc tggacggcgg tggtctgggg
    12481 gcatctgctg tgggtaatgc cgtggatgct gtttatcctg caacccgcct ggcagcgcat
    12541 tgattcacgg ttaattttga ttgcgcaaac actgggctgg tcgcgggcca aaatcttctt
    12601 ttacgtaaaa tgcccactca tgttgcgccc tgcgctgatt gccttcgcgg tgggattttc
    12661 agtcagtatt gcgcagtata tgccgacgct gtggctgggc gcggggcgtt ttccgacgct
    12721 caccactgaa gcggtggcat taagcagcgg cggcagcaac ggtattctcg ccgcccaggc
    12781 tttatggcaa ctgctgttac cgcttattat ttttgccctg accgcgttag tcgtaaaatg
    12841 ggtaggttat gtcagacaag gactccgcta atgctctgcg tgaaaaatgt ttcgctacgt
    12901 ttaccagaaa gccgcttgct gacaaacgtt aactttacag tagataaagg tgacattgtc
    12961 acgttaatgg gaccatccgg ctgcgggaaa tccactctgt tttcgtggat gattggtgcg
    13021 ctggccggac agttttcttg tacaggggag ctatggctta atgagcaacg gattgacatg
    13081 ctacccaccg cacagcgtca gattggcatt cttttccagg atgcactgtt atttgaccag
    13141 ttcagcgtcg ggcaaaattt actgctggcg ctaccggcga cacttaaggg gaatgcccga
    13201 cgtaatgccg tgaatgatgc acttgagcgt tcaggccttg acggagcgtt ccatcaggat
    13261 cctgccactt tgtctggcgg tcagcgagcg cgcgttgctc tgcttcgcgc ccttctcgct
    13321 cagccaaaag cgttgctact ggatgagcca ttcagccgtc ttgatgtggc cctgcgcgat
    13381 aattttcgcc attgggtgtt cagcgaaatt cgcgccatgg cgatccctgt tgtgcaggtg
    13441 acgcacgatc ttcaggatgt tcctcctgat agccccgttc tggatatggc gcagtggtca
    13501 gaaaattaca acaaactgcg ataacgcaaa gtttttcccg atgcgtcagt tcagaatggc
    13561 gctctcaaaa ctacaatgtc gggattttcg atgaaacgtg tttctcaaat gaccgcgctg
    13621 gcaatggctt tagggctggc ttgcgcttct tcgtgggccg ctgaactggc gaagcctctt
    13681 acacttgacc agcttcaaca acaaaatggc aaagcgatag atactcgccc cagcgcgttt
    13741 tataacggct ggccacaaac cttaaatggc ccttccggtc atgaacctgc cgccttaaac
    13801 ctctctgcta gctggctcga caaaatgagc accgaacagc tcaacgagtg gatcaagcaa
    13861 cataacctga aagccgatgc cccggtggca ctgtacggta atgacaaaga tgtcgacgcc
    13921 gtcaaaacgc gactgcaaaa agcaggtttt acgcatatct ccatcctgag tgacgcgcta
    13981 agcgaacctt cccgtctgca aaaactgccg cactttgaac agctggtata tccgcagtgg
    14041 ctgcatgacc tgcaacaagg taaagaggtt acggcgaaac ctgccggtga ctggaaagtc
    14101 attgaagcgg cctggggcgc tccaaaattt taccttatca gccatattcc cggcgctgac
    14161 tacatcgaca ccaacgaagt ggaaagcgaa ccgctgtgga acaaagtttc tgatgaacaa
    14221 ctgaaagcga tgctggcaaa acacggcatt cgccatgaca ccacggtcat tctgtacggg
    14281 cgtgacgtat acgctgcagc gcgtgtggcg caaattatgc tttatgccgg cgtgaaagat
    14341 gtgcgcctgc tggacggagg ctggcaaacc tggtccgacg cgggtctgcc cgttgagcgc
    14401 ggtacgccac cgaaagtgaa agcggaaccg gatttcggcg tgaagatccc ggcacaaccg
    14461 caactgatgc ttgatatgga acaagcgcgt ggactgctgc atcgccagga tgcatcgctg
    14521 gtgagcattc gttcgtggcc agaatttatc ggtacgacca gcggttacag ctatattaaa
    14581 ccaaaaggtg aaatagccgg agcacgttgg ggacacgcag gtagcgactc gacgcatatg
    14641 gaagatttcc ataacccgga tggcaccatg cgcagcgccg atgatataac cgctatgtgg
    14701 aaagcatgga atatcaaacc agatcagcaa gtttcattct actgcggcac tggctggcgc
    14761 gcgtcagaaa cctttatgta cgcacgcgca atgggctgga ataacgtctc cgtttacgac
    14821 ggtggctggt acgaatggag cagcgatcca aaaaatccgg tagcaaccgg tgaacgcggc
    14881 ccggacagca gtaaataaca ttgcaattac tgacgctgga gcgacttcag cgtcagatac
    14941 ccactccaca cccgcgtaaa ggtcgtcatc cagcacaacg caccaaatac ccacgcaaac
    15001 cacggaaaac aggccggaaa gaggcaactc agaacaaaca gcaaaatcgt ctcgctgcct
    15061 tcggttaagc cgcccagata ataaaacgat ttatgcgcat aaccaggatt atcaatctga
    15121 tgtttcgccg ccagcgcagc aaaagcgaga aaactgctgc ccgtgccgat aaacgcaaac
    15181 agcagccagc cgcctgccag ggcgttttgc tccggtgcgg cgaggataaa cccaaaaggc
    15241 accagcgcgt aaaaaagaaa atcgagagaa atatcgagaa agccgcccgc gtcggtaagc
    15301 cctctgcgcc gcgccagcgc accgtccaga ccatcaagca gcctgttcaa caaaatggcg
    15361 actagtgccg ccagatacca gcccagcgcc agaaaaggca gtgccagcac gccgatggca
    15421 aatcccacta atgttaaacc atctggcgta atgcccggtt tatcaagaac ccgcacgcac
    15481 tgatgcaaca acggtttaat ccggggatga agatggcggt ctagcacagg cactccttaa
    15541 atataaagcc tttctgattg agcaacagtg cggatattat ggcatttttc gcttatctgc
    15601 ccgtgtgtaa tttatgaaaa tgattgaagt tgttgccgcc atcatcgaac gtgatggcaa
    15661 aattttactc gcgcaacgcc ctgcccagag cgatcaggcg ggattatggg agtttgccgg
    15721 tggtaaaatc gagccggatg aaagccagcg gcaggcgctg gtgcgtgagt taaacgaaga
    15781 actgggcatc gaagcaactg ttggtgacta tgttgccagc catcagcgag aagtttcggg
    15841 gcggattatc catcttcatg cctggcacgt acccgacttc cacgggacgt tacaggcaca
    15901 tgaacatcag gcgctggtct ggtgctcacc tgaagaggcg ctgcgatatc cgctggcccc
    15961 tgctgacatt ccattattag aggcgtttat ggcttcacgc gccgccagac cagcggatta
    16021 gtgcttaggg ttttgtcatc ccgttggcat tgcagcagta ttccttcggc tttaattact
    16081 gccccttcag aataattttg atcctgataa acgcagcact gagtacaggg ctgcgctgac
    16141 tgcccgcctg aactgaacac ttctggcggt acgtttacct ccacgtccgg atgataatgc
    16201 gggttagcca gtgtaattaa tggaaatgct aatactacgg cgatcaatgc tcgactcaca
    16261 gggaactcct taacgttatt gtctcttcta cagataacgg tagccgggcg gcaaaacttt
    16321 agcgtctgag ttatcgcatt tggttatgag attactctcg ttattaattt gctttcctgg
    16381 gtcatttttt tcttgcttac cgtcacattc ttgatggtat agtcgaaaac tgcaaaagca
    16441 catgacataa acaacataag cataatcgta ttaatatata agggttttat atctatggat
    16501 cagacatatt ctctggagtc attcctcaac catgtccaaa agcgcgaccc gaatcaaacc
    16561 gagttcgcgc aagccgttcg tgaagtaatg accacactct ggccttttct tgaacaaaat
    16621 cccaaatatc gtcagatgtc attactggag cgtctggttg aaccggagcg cgtgatccaa
    16681 tttcgcgtag tatgggttga tgatcgcaac caggtgcagg tcaaccgtgc atggcgtgtg
    16741 caattcagct ccgccatcgg tccgtacaaa ggcggtatgc gcttccaccc ttcagtaaac
    16801 ctttccattc tcaaattcct cggcttcgag caaaccttta aaaatgccct gactactctg
    16861 ccgatgggtg gtggtaaagg cggcagcgac tttgatccga aagggaaaag cgaaggcgaa
    16921 gttatgcgtt tctgccaggc gctgatgact gaactgtatc gccacctggg cgcggatact
    16981 gacgttccgg caggtgatat cggcgttggt ggtcgtgaag tcggctttat ggcggggatg
    17041 atgaaaaagc tctccaacaa taccgcctgc gtcttcaccg gtaagggcct ttcatttggc
    17101 ggcagtctta ttcgcccgga agctaccggc tacggtctgg tttatttcac agaagcaatg
    17161 ctaaaacgcc acggtatggg ttttgaaggg atgcgcgttt ccgtttctgg ctccggcaac
    17221 gtcgcccagt acgctatcga aaaagcgatg gaatttggtg ctcgagtgat cactgcgtca
    17281 gactccagcg gcactgtagt tgatgaaagc ggattcacga aagagaaact ggcgcgcctt
    17341 atcgaaatca aagccagccg cgatggtcga gtggcagatt acgccaaaga atttggtctg
    17401 gtctatctcg aaggccaaca accgtggtct gtaccggttg atatcgccct gccttgcgcc
    17461 acccagaacg aactggatgt tgacgccgcg catcagctta tcgccaatgg cgttaaagcc
    17521 gtcgccgaag gggcaaatat gccgaccacc atcgaagcga ctgaactgtt ccagcaggca
    17581 ggcgtactgt ttgcaccggg taaagcggct aatgctggcg gtgtagcaac atcgggcctg
    17641 gaaatggcgc aaaacgctgc gcgcctgggc tggaaagccg agaaagttga cgcacgtttg
    17701 catcacatca tgctggatat ccaccatgcc tgtgttgagc atggtggtga aggtgagcaa
    17761 accaactacg tgcagggcgc gaacattgcc ggttttgtga aggttgcgga tgcgatgtta
    17821 agccagggcg tgatttaaga caattgtgcc ggatacagcg caaacgcctt atccggccat
    17881 aaaaatcttc agtatcagat ggttgaaaga acctttatat tatcactcca ttgcccgcct
    17941 cttctgatgg cgggcttttt ttcggcgctc tcttgcgtgg cgaatccttt ttcttccctt
    18001 ctccaccgcc gggagccatt attccccgaa actgccttac cgacgtgcgc tttgcctgat
    18061 caatcaactg gtacaatgtc cccaccagcg gctgcataaa gtcctgatag cgacactgct
    18121 tttcgctgat ttgcgtcagc accgattccc agtgcgcggt catgtccggt cgcgtcgcca
    18181 tttctggcag cgaatggaat agcgcttttc cggcgtcggt ggagtggata tagcgccctt
    18241 ttttggtcag gaaaccccgc ttgaacaaca gttcaataat cccggcacgc gttgcttctg
    18301 tccccagacc atcggtcgca cgaaggatct ttttcagatc tttgtcctgc acaaagcgcg
    18361 cgatcccggt catcgccgaa agcagtgttg catcggtaaa atggcgcggc ggctgggttt
    18421 gccgctctac cacttcgcct ttttcgcaca acaattcatc gcctttcgcc accacaggca
    18481 gcggcgtgcc gtcgttttct tcatcgcgct ctttgctgcc taacagcgtg cgccagcctg
    18541 cttcagcaag aaaacgggct ttagcgacaa atttgccttt ggcaatgtcc agttcgataa
    18601 cacacttgcg gaacaccgca tccgggcaga actgcatcaa gtactgacgg gcaatcaggt
    18661 tatagacctt cgcttcgttc tccgtcaggt tgatcgcaga actccgtgcg gtcggaatga
    18721 tggcgtggtg cgcatcgacc tttttgtcat cccaacagcg gttgcgtata tctggatcta
    18781 ccactggctg cggcaacaga tccggtgcat gaacgctgat agcattcatc accgcgtggc
    18841 gtccggcaaa atgttcttct ggcaaatagc ggcaatcgga acgcggataa gtgattagct
    18901 tgtacgtttc gtacagcttc tggcagatat caagcacgtt ctgcgcgctc agaccaaaac
    18961 gtttagccgc ttcaatctgc aacgccgaaa gcgaaaaagg cagcggcgcg gattctgatt
    19021 cccgtttatc gttatagctg gtgacaatag ctggttgacc actaatacgg ttaaccacat
    19081 gctccgccag tggacgatgt aacaagcgcc cttcttcatc ctggtacggt tcacacgctt
    19141 cgctcggttg ccagatagcg gtaaaccgct catcggcagg tgtcacgata tgtgctttga
    19201 cttcaaagaa gtctttcgcc acgaagtttt caatctcttc gtcgcggcgc accaccagtc
    19261 cgagtacggg cgtctgcacg cgtcccacgg aaagcacacc ctgataaccg gcattgcggc
    19321 cgagaatggt atacgcacgg gtcatattaa tgccgtacag ccagtcagcc cgcgcccgcg
    19381 ccagtgcaga aacgcacagc ggtacaaact cgctattgga gcgaagacga tcgatcgccc
    19441 gctcaaccgc ctgcgggttc aagtcgttta tcaaacaacg ctgtacctgc tggcgctttt
    19501 ccggtgccag ttgcaggtag tccagcactt catccaccag cagttgccct tcgcgatccg
    19561 ggtctccggc gtgaacgatt tcgctggctt catgcaggaa gcgtttgatg acattaagtt
    19621 gtttagtcac ggagggtcgg ggctgtaatt gccacttttc cgggacaatt ggcaaatccg
    19681 caagattcca gcgcgcatag cggctgtcgt aggcgtctgg ctgcgcctgc tcaagcaggt
    19741 gaccgataca ccaggtcacc acctgaccat taccgcactc gataaagcca tcgcctttcc
    19801 ggtgcggttt aggcaggaca tcagcaatgg cgcgcgccag actcggtttt tcggcaataa
    19861 acaaccgcat tgaattaacg gatctcaacc atggcacgac cgccgcgcgc tggcaccagt
    19921 tcgccaatcg ccgtcagttc aatgccaaac tcggcggctg tagctttgac ctcattttct
    19981 gcttccggca tgaccgccag cagcaaaccg ccagaagttt gcggatcgca cagcagatcg
    20041 cgcacttcac gcggcatttc acccatcaga tgaccgtagc tggcaaagtt acgttcggtg
    20101 ccgccaggca ctgcgcccaa cttaatgtac tcttcaaccc ccggcaattt cgggatcgcg
    20161 tcatagtcga cgcgcgcctg cacacctgcg ccctgacaca tttcgcttaa atgccccagt
    20221 aaaccaaaac ccgtaacgtc ggtcatcgct tttacgcctt cgatgttggc aaaggacacg
    20281 cctgcgatgt tcatccggca catcacttcc gtcgccagtc cctgatgttc tggtttcagc
    20341 agtgattttt tctcagccgt ggtaagaacg ccaatcccca gcggtttcgt caggaacagt
    20401 ttgcatccgg cttgtgcggt actgtttttc ttcacccgct cggtcggtac gatccccgtt
    20461 accgccagac caaaaatcgg ctccggcgca tcgatggagt gaccgccagc cagcgcaata
    20521 cccgcctgac ggcatgcata gcgtccacct tcggtcactt cgcgggcaat ttccggggaa
    20581 agtttgttaa tcggccagcc gaggatcgca atcgccataa tcggtttgcc gcccatagca
    20641 aagatatcgc tgatggcgtt agtcgccgca atgcggccaa aatcgaaagg attatcaacg
    20701 atcggcataa agaagtcggt ggtactgata acgctggtgc cattgcccag atcgtacacc
    20761 gccgcatcgt cgcgggtttc attacccaca agcaaattcg gatcaacaaa cttcgcctgc
    20821 tcactgtgca ggatggtttc caacactttt ggggaaattt tacagccgca accagcgccg
    20881 tggctgtatt gggtcaaacg aatcgagttc tcgctcatgg acatctcctg tcaatgcaat
    20941 ccgggtatgg taaccctcat tccatgaagt gataagtgag agtgtctgaa ttcctgcgcc
    21001 tttgctcaca atccagacag tttcgcgaca attatcagaa ataagtcaca aacggcgtcg
    21061 ggtccgggac gtttatcgac gtagatgctt tcaactgcgg cgtaccgagg tagagaaaac
    21121 cgacaatttt atcctgctca cggcaaccga atgcttcacg cactaccgga ctttcagtta
    21181 atgcaccact gcgccagatg ccgccaaacc cctgggcaac tgctgccatt tgcatcgcca
    21241 tgaccgcgca tcccgcagac atttcctgtt cccagcgcgg gactttatga ttttcttcac
    21301 atttcgccac caccgttatg atgagcggtg cgcggaacgg cgcattacgc gctttgtcga
    21361 ttgctttgtt atcactaccg gcagcaatcg ccccctgctc cagtacggcg ctgaaacgct
    21421 cgcgcccttc cccttcaatc acaaagaaat gccacggttg catggactta tggtccggcg
    21481 cacgcatacc cgcacgcagg atgttttgca gttgttcacc cgttggcgcg ggttcagcca
    21541 ggcgggaggc gctacggcga ttgatcaata gttcgagtgc atccatttga ttaactcctg
    21601 tcatgatatt tattcacaaa attaacacga gagtggattt tgttacagcc cagtccgcaa
    21661 ttcctgctga caagtgccgg ttgggtcatt acgataacca catctattgc gcctgaaaca
    21721 ggtgtgacct taagttggga gaatacatgc gaaccctttg gcgatttatt gccggatttt
    21781 ttaaatggac gtggcgtctg ctgaatttcg tccgtgaaat ggtacttaac ctgttcttta
    21841 tttttctcgt gctggttggt gtggggatct ggatgcaggt cagtggtggt gattcgaaag
    21901 aaacggccag tcgtggcgca ctgctgctgg acatttctgg tgtgatcgtc gataaacccg
    21961 acagttctca gcggtttagt aaattaagcc gccagctgct tggtgccagt tccgatcgtc
    22021 ttcaggaaaa ctcactgttt gatatcgtca acaccattcg ccaggcgaag gacgaccgca
    22081 atatcaccgg tattgtgatg gatctgaaaa acttcgcagg cggcgaccag ccgtctatgc
    22141 agtacatcgg caaagctctg aaagagtttc gtgacagcgg gaaaccggtt tatgccgttg
    22201 gcgagaacta cagccagggg caatattatc tcgccagttt cgccaataaa atttggctgt
    22261 ctccgcaagg cgtggttgat ctgcacggct ttgccaccaa cggtctgtac tacaaatcgt
    22321 tactggataa gctgaaagtt tccacccatg tgttccgcgt gggtacgtat aaatctgccg
    22381 ttgaaccgtt tattcgtgat gatatgtcgc cggcagcccg cgaagctgac agccgctgga
    22441 ttggtgagct gtggcaaaac tatctgaata ctgttgccgc taaccgccag atccctgctc
    22501 agcaggtatt ccctggcgcg caagggttgc ttgagggttt aaccaaaacc ggtggcgata
    22561 ccgcgaaata tgcgctggaa aacaagctgg tcgatgcact ggcatcgagt gccgaaatcg
    22621 aaaaaactct gaccaaagag ttcggctgga gtaaaactga taaaaattat cgcgccatca
    22681 gttattacga ttacgcattg aaaacgccgg cagataccgg tgacagtatc ggtgtcgtct
    22741 tcgctaatgg cgcaattatg gatggcgagg aaactcaggg gaatgttggc ggtgatacca
    22801 ctgcggcaca aatccgcgac gctcgccttg acccgaaagt gaaagcgatt gttctgcgtg
    22861 ttaatagtcc cggcggcagc gttaccgcgt ctgaagtgat tcgcgctgaa ctggcagcag
    22921 cccgggcagc gggtaagcct gtggttgtat cgatgggtgg catggcggca tctggtggtt
    22981 actggatttc cacaccagct aattacattg tggctaaccc cagcaccctg accggttcta
    23041 tcggtatctt cggcgtgatc accaccgtag aaaatagtct ggattcgatt ggtgttcata
    23101 ctgatggtgt ctcaacttca ccgctggcgg atgtttctat caccagggca ctgccgccgg
    23161 aagtgcagca gatgatgcag ttaagcattg agaatggcta taaacgcttt atcacgctgg
    23221 ttgctgatgc gcgtcattca acgccggagc agattgataa aatcgcccag ggccacgtct
    23281 ggaccggtca ggatgcaaaa gctaacgggc tggtcgatag tctcggggat ttcgatgatg
    23341 cggtcgccaa agcagcagag ctggcaaaag tgaaacagtg gcatctggaa tactacgttg
    23401 atgaaccgac cttcttcgac aaagtgatgg acaacatgtc tggttctgtc cgggcaatgt
    23461 tgccagatgc gttccaggcc atgttacctg caccgctggc ctcggtagcc tctaccgtta
    23521 aaagtgaaag cgacaagctg gccgcgttta acgacccaca aaaccgttat gcgttttgcc
    23581 tgacctgcgc caacgtgcgt taagtcctgc aaaaatgtgg ccgatatgct gtcggccaca
    23641 ctcctgaatc ggactgtctt tcactccctc tctccactac attcccttgc tcatttagca
    23701 tctgatatgg agtcatcagt gaataaggcc ccctctttaa ttgccgctat cgtcctcgga
    23761 ctggggatta gcgcctgtgg ttattttgtt ggcgatggtg ttaaacattt aaaaaccaat
    23821 aaccgttatg taaatgtgcg tgggctttct gaaaaagaag ttcgcgcaga tacggcagaa
    23881 ttaacaattg cgattaactt taagggtaat attcccggcg aactgttccc gaaactggaa
    23941 gacgcgcaga aaaaaattgt tgccgaactc aatgctcagg ggattaacga gaaagagata
    24001 accctgggcc aatggacaag caagcgaaca gattcctttt atctgaaaga tgatcccaca
    24061 atgccacgct ataacgcaga cgggtcagtg acgataaaaa cccataatgt tgctgctgtc
    24121 gaaaaagtcg tcgcgaaatt aaacgaactg caagtcgcta cggagggcgc tatcgctgag
    24181 agcaaagtcg cctatcgctt taatggtatt ggcgcactgc gcgctgagat gattgccgcc
    24241 gccaccaaag atgcacgcaa cgcagcatta caatttgcaa cggactcagg tagccaggtg
    24301 gggtcaatta gcgatgcctc acaaggcgtg ttccagatct tcgccagtgg cagtgatgaa
    24361 gatgatccta cggcgattaa taaaacggta cgcgtagtca ccaccgtaac ctatgcgttg
    24421 caggattaac cacttttctc cggcgggaca ctaatccctc cgttctgccg gacttttctc
    24481 atatccacag agtatccagg tgcgctcgct gcccctcacg tatatacttt tgctctttcg
    24541 atatcattca tatcaatatc atgcaaaaga aatcaattta cgtcgcctac acgggcggga
    24601 ccatcgggat gcagcgttcc gagcagggtt atattccggt gtcaggtcat ctacagcgcc
    24661 aactggcgct gatgccggaa ttccatcgcc cggagatgcc agatttcacc attcatgaat
    24721 atacgccatt gatggattct tctgatatga cgccagaaga ctggcagcat attgctgaag
    24781 atattaaagc gcactatgac gactatgatg gttttgtcat tctgcacggc acagacacga
    24841 tggcgtatac cgcctctgca ctgtcgttca tgctcgagaa tctcagtaaa ccggtcattg
    24901 tgacagggtc acaaatcccg ctggctgagt tacgctctga cggacaaatt aatctgctga
    24961 atgcgttgta cgttgcggcg aattatccta tcaacgaagt aactctcttt tttaataatc
    25021 gactgtatcg tggcaaccga accaccaaag cccatgccga tggttttgat gcgtttgcct
    25081 ctccaaacct tcctccgtta ctggaagcag gtatccatat tcgtcgtttg aatacgccac
    25141 ccgccccgca cggcgaaggg gaattgatcg ttcatccaat cacgccgcaa ccaattggcg
    25201 tagtgacgat ttatccgggg atttctgctg acgtcgtgcg caattttctg cgccaacctg
    25261 tgaaagcatt gattctgcgc tcatatggcg tgggtaatgc gccacaaaac aaagccttcc
    25321 tgcaggaatt acaagaagcc agcgatcgcg gtattgtggt ggtcaacctg acacaatgta
    25381 tgtccggtaa agtgaacatg ggtggttatg ccaccggtaa cgccctcgcc catgccggcg
    25441 ttattggcgg tgcagatatg actgtagaag ccacgctaac caaactgcat tacctgctta
    25501 gccagaaact tgatactgaa accattcgca aggccatgag ccaaaacctg cgtggcgaac
    25561 tgacgccgga tgattaagga gactgcaatg ccccctcgcg ccctgttact ggtcgattta
    25621 caaaatgatt tctgtgctgg tggcgcgctc gccgtgccgg aaggtgacag tacggtggat
    25681 gtcgctaacc gcctgattga ctggtgccag tcgcgcggtg aagcagttat cgccagtcag
    25741 gactggcatc cggcgaatca cggcagtttt gccagtcagc acggtgtaga gccttatacg
    25801 ccaggccaac tcgacggttt gccacaaacc ttctggcccg atcactgtgt gcagaacagt
    25861 gaaggcgcac aattacatcc gttactgaag caaaaagcga tcgcagcggt gttccataaa
    25921 ggcgaaaatc ctttagttga cagttacagt gccttttttg ataacggtcg tcggcagaaa
    25981 actgcgctcg atgactggtt gcgcgctcat gtaatcaatg aattgatcgt tatgggtctg
    26041 gctactgact attgcgttaa gtttaccgtg ctggacgctt tacagttagg ctataaggtg
    26101 aacgtgatta ccgatggttg tcgtggcgta aatatccagc cacaggacag tgcgcacgcg
    26161 tttatggaaa tgtcagcagc tggggcaacg ctatatacgc tggcagactg ggaagaaacg
    26221 caggagtaat tttacgctgg cctacaattc tgtactggca ttgtaggcca gataaaacac
    26281 atcagtgata catctggtaa ttgacgccat caacgaaaga tttaattcac ctcagaaatc
    26341 tcttccagcg acaccttccg cgtttcgatg ccaaaaatgg agagaaccag cgcacagagc
    26401 actaacatga caccaagcac cataaacacc gtgatcgacc cataatgtgt taataatgcc
    26461 gcaacgccat agggcgtgaa gactgcgacg atccgtccta cggcattaac gaaacccgaa
    26521 ccacgcaggc gtaaatgcgt tggccaaagt tccgggatat aaaccgccga cgcgaagcaa
    26581 acatacatgt ataaaaagaa gatcatcacc aggccataga ttaaaatcgc ccactctgtt
    26641 gtctgaattg aatagatata gcctaacacg gcaataataa taagtaaggc ggaaccaaat
    26701 aatcgacgag ggaaatgatc aataattaat gccgcaataa atatccctac cggagcgcca
    26761 atcataataa cagcggtcat taatattgat ttatcgacat caatgccgga gttaacaaat
    26821 atggtcggca tccatacggt gatggtataa agcgaaatgt tcattgcaat taaaacagta
    26881 atcgcgacta atgtacgtcg taacatttcc cctttaaaca ggagccagaa ggtacccttg
    26941 attaccttaa ccttgctgtt gctctggttc aaagtaagcg ggggtaaacg aatactcttc
    27001 tctctttcaa tttgctgctc tacttcacga agttggcttt ctgcacctgc gattcgccct
    27061 ttccctgcca accatcgtgg cgactcaata aagtatttac ctgagagaaa ccaggctaac
    27121 agtatgccaa taccgcccag cagaaacatt atccgccaac taaaaaaagc gataaccacc
    27181 acgcctatcg ccgcagacag catgggcgac cagtttccaa caaatgagag ccgcgcggac
    27241 cattttccac gcaccgtcgc ggggataaac tccgtaaatg aggcatagcc aaccataatc
    27301 agcgccccca ttcctgttcc catcaggaag cgaaagaaga tgagccagta catatcaggg
    27361 acaaaagcgg cccctgttgc cgcaataccg acgatgagaa gatttatgcg aaacgccctg
    27421 cgccgcccaa agtagtcacc aataaaccca ccggtaagag agccgatgaa ataaccaaac
    27481 attaatgccg aggtaaaggc ggcattgagg aaattatttg accatccatt gcttaccagc
    27541 tttgctaaga cgacattacc ggagtaactc aaaaaccccg ttaataacag actaaagctt
    27601 attataccaa atatacgata atgaaaccgg gccaaaggca agcggtccag tcttgcgcca
    27661 atttgatcat attgttccat tgggtgcctc ggatttgcta aaaaaggtag caattgcggc
    27721 aaattcactt gccgcatctt tatcatcatt ttatttccag gcgcaggaac aaataatatt
    27781 gacaatgtgt gcttaaatta ccacaccagc tggatattat tgtctttgca aaatg
//
LOCUS       DACADF010000057        27750 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.057, whole genome shotgun sequence.
ACCESSION   DACADF010000057 DACADF010000000
VERSION     DACADF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.057"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            125..628
                     /gene="dps"
                     /locus_tag="ISB30_002265"
                     /gene_synonym="pexB"
     CDS             125..628
                     /gene="dps"
                     /locus_tag="ISB30_002265"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAP1989895.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1032..1778
                     /gene="glnH"
                     /locus_tag="ISB30_002266"
     CDS             1032..1778
                     /gene="glnH"
                     /locus_tag="ISB30_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAP1989896.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1917..2576
                     /gene="glnP"
                     /locus_tag="ISB30_002267"
     CDS             1917..2576
                     /gene="glnP"
                     /locus_tag="ISB30_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAP1989897.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2573..3295
                     /gene="glnQ"
                     /locus_tag="ISB30_002268"
     CDS             2573..3295
                     /gene="glnQ"
                     /locus_tag="ISB30_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAP1989898.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3412..5637
                     /gene="ybiO"
                     /locus_tag="ISB30_002269"
     CDS             3412..5637
                     /gene="ybiO"
                     /locus_tag="ISB30_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAP1989899.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYCKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKTVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(5634..6560)
                     /gene="rlmF"
                     /locus_tag="ISB30_002270"
     CDS             complement(5634..6560)
                     /gene="rlmF"
                     /locus_tag="ISB30_002270"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAP1989900.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6836..7096
                     /locus_tag="ISB30_002271"
     CDS             6836..7096
                     /locus_tag="ISB30_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1989901.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            7361..9643
                     /locus_tag="ISB30_002272"
     CDS             7361..9643
                     /locus_tag="ISB30_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HAP1989902.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQKSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGFSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPTVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9685..10362
                     /gene="ybiX"
                     /locus_tag="ISB30_002273"
     CDS             9685..10362
                     /gene="ybiX"
                     /locus_tag="ISB30_002273"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAP1989903.1"
                     /translation="MMYHIPGVLSPQDVARFREHLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSHYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10436..10702
                     /gene="ybiI"
                     /locus_tag="ISB30_002274"
     CDS             10436..10702
                     /gene="ybiI"
                     /locus_tag="ISB30_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAP1989904.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            10967..11227
                     /locus_tag="ISB30_002275"
     CDS             10967..11227
                     /locus_tag="ISB30_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1989905.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(11365..12450)
                     /locus_tag="ISB30_002276"
     CDS             complement(11365..12450)
                     /locus_tag="ISB30_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAP1989906.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKVVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(12590..13552)
                     /gene="ybiB"
                     /locus_tag="ISB30_002277"
     CDS             complement(12590..13552)
                     /gene="ybiB"
                     /locus_tag="ISB30_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAP1989907.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATISDGLARVNQAF"
     gene            complement(13580..15730)
                     /gene="dinG"
                     /locus_tag="ISB30_002278"
     CDS             complement(13580..15730)
                     /gene="dinG"
                     /locus_tag="ISB30_002278"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAP1989908.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGALLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            15834..16034
                     /locus_tag="ISB30_002279"
                     /pseudo
     CDS             15834..16034
                     /locus_tag="ISB30_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1768 domain-containing protein"
     gene            complement(16267..17628)
                     /gene="rhlE"
                     /locus_tag="ISB30_002280"
     CDS             complement(16267..17628)
                     /gene="rhlE"
                     /locus_tag="ISB30_002280"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HAP1989909.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAILGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            17857..18528
                     /gene="cecR"
                     /locus_tag="ISB30_002281"
     CDS             17857..18528
                     /gene="cecR"
                     /locus_tag="ISB30_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAP1989910.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            18528..19526
                     /gene="hlyD"
                     /locus_tag="ISB30_002282"
     CDS             18528..19526
                     /gene="hlyD"
                     /locus_tag="ISB30_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAP1989911.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            19519..21255
                     /locus_tag="ISB30_002283"
     CDS             19519..21255
                     /locus_tag="ISB30_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1989912.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            21248..22381
                     /locus_tag="ISB30_002284"
     CDS             21248..22381
                     /locus_tag="ISB30_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1989913.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANAIAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLIVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            22392..23498
                     /locus_tag="ISB30_002285"
     CDS             22392..23498
                     /locus_tag="ISB30_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1989914.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(23460..23870)
                     /locus_tag="ISB30_002286"
     CDS             complement(23460..23870)
                     /locus_tag="ISB30_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989915.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            24003..24764
                     /locus_tag="ISB30_002287"
     CDS             24003..24764
                     /locus_tag="ISB30_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP1989916.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGESVLVAGDFNDWRQKANHPLKVLAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            24761..26002
                     /gene="clsB"
                     /locus_tag="ISB30_002288"
     CDS             24761..26002
                     /gene="clsB"
                     /locus_tag="ISB30_002288"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAP1989917.1"
                     /translation="MKCSWREGNMIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSTARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            26002..26958
                     /locus_tag="ISB30_002289"
     CDS             26002..26958
                     /locus_tag="ISB30_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAP1989918.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVSYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(26994..27707)
                     /locus_tag="ISB30_002290"
     CDS             complement(26994..27707)
                     /locus_tag="ISB30_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP1989919.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPQMYVAIGLLLLSMYGLSKDINKINTAIAGVIYLFLISLVAIVVASLAP
                     VSAIIVVFSTAGSMFLISMLAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELTGKSHSEELWSPVRCAFIGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
BASE COUNT         6561 a         7364 c         7228 g         6597 t
ORIGIN      
        1 tttgtttttc acgcttgtta ccactattag tgtgatagga acagccagaa tagcggaaca
       61 catagctggt gctatactta gtctcgttaa ttactgggac ataacatcaa gaggatatga
      121 aattatgagt accgctaaat tagttaaatc aaaagcgacc aatctgcttt atacccgcaa
      181 cgatgtctct gacagcgaga aaaaagcaac agtagagttg ctgaatcgcc aggttatcca
      241 gtttattgat ctttctttga ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt
      301 cattgccgta catgaaatgc tggatggctt ccgcactgca ctgatcgatc atctggatac
      361 catggcagaa cgtgcagtgc agctgggcgg tgtagctctg gggaccactc aagttatcaa
      421 cagcaaaact ccgctgaaaa gttacccgct ggacatccac aacgttcagg atcacctgaa
      481 agagctggct gaccgttacg caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc
      541 gaaagatgac gacaccgcag atatcctgac cgccgcgtct cgcgacctgg ataaattcct
      601 gtggtttatc gagtctaaca tcgaataaat ccatcgctga tggtgcagaa ctttagtaca
      661 cgataaaagc ggcttcctga caggaggccg ttttgttttg tagcccgcct caacgcactt
      721 atttagtgca tccatctgct atccccagct ggttaagtaa attttttgta tccacatcat
      781 cacacaatcg ttacataaag attgtttttt catcaggttt tacgctaaat aatcactgtg
      841 ttgagtgcac aattttagcg caccagtttg gtgccccaga atggtgcatc ttcagggtat
      901 tgccctataa atcgtgcatc acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa
      961 ctggcacgat tttttcatat atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa
     1021 aaggaaatgc tatgaagtct gtattaaaag tttcactggc tgcactgacc ctggcttttg
     1081 cggtttcttc tcatgccgcg gataaaaaat tagttgtcgc gacggatacc gccttcgttc
     1141 cgtttgaatt taaacagggc gataaatacg tgggctttga cgttgatctg tgggctgcca
     1201 tcgctaaaga actgaagctg gattacgaac tgaagccgat ggatttcagt gggatcattc
     1261 cggcactgca aaccaaaaac gtcgatctgg cgctggcggg cattaccatc accgacgagc
     1321 gtaaaaaagc gatcgatttc tctgacggct actacaaaag cggcctgtta gtgatggtga
     1381 aagctaacaa taacgatgtg aaaagcgtga aagatctcga cgggaaagtg gttgctgtga
     1441 agagcggcac tggctccgtt gattacgcga aagcaaacat caaaactaaa gatctgcgtc
     1501 agttcccgaa catcgataac gcctatatgg aactgggcac caaccgcgca gacgccgttc
     1561 tgcacgatac gccaaacatt ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag
     1621 cggtaggtga ctctctggaa gcacagcaat acggtattgc gttcccgaaa ggtagcgacg
     1681 agctgcgtga caaagtcaac ggcgcgttga aaaccctgcg cgagaacgga acttacaacg
     1741 aaatctacaa aaaatggttc ggtactgaac cgaaataata acgctgcgcc tgtaaagcgc
     1801 actggcagtt ccctctcccc gccggggaga ggattagggt gaagggcgca aacccgctcc
     1861 ggggccatta attaccctga atttgattat ttacaccacg gtaacaggaa caacatatgc
     1921 agtttgactg gagtgccatc tggcctgcca ttccgcttct gattgaaggt gccaaaatga
     1981 ccctgtggat ttcggtcctc ggtctggctg gcggtctggt gatcggattg ttggcaggtt
     2041 ttgcacgcac cttcggtggt tggatagcca accacgtcgc gctggtcttt attgaagtga
     2101 tccgcggcac acctatcgtc gtccaggtga tgtttatcta tttcgccctg ccgatggcgt
     2161 ttaacgattt acgcatcgac ccgtttactg cggcggtggt caccatcatg atcaactccg
     2221 gcgcgtacat tgcggaaatc acgcgtggtg cggtgctgtc tattcacaaa ggttttcgtg
     2281 aagcaggact ggcgctcggt ctttcacgtt gggaaaccat tcgctacgtc attttaccgc
     2341 tggcactgcg tcgtatgctg ccgccgctgg gtaaccagtg gatcatcagc attaaagaca
     2401 cttcgctgtt tattgttatc ggcgtggcgg aactgacccg tcaggggcaa gaaattattg
     2461 ccggtaactt ccgcgccctt gagatctgga gcgccgtggc ggtgttctat ctgattatta
     2521 ccctggtgct gagctttatt ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga
     2581 atttaaaaac gtctccaagc attttggtcc aacccaggtg ctgcacaata tcgatttgaa
     2641 cattgctcag ggcgaagtcg tggtgattat cgggccgtcc ggttccggta aatcgaccct
     2701 gctgcgctgc atcaacaaac tggaagaaat cacctccggc gatctgattg tcgatggcct
     2761 gaaggttaac gatccgaaag ttgatgagcg cctgattcgc caggaagcgg gtatggtgtt
     2821 ccagcagttt tacctcttcc cgcatctgac ggcgctggaa aacgtcatgt ttggcccgct
     2881 acgcgtgcgt ggcgcgaata aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa
     2941 agtcggtctg gcagagcgtg cacatcacta cccttccgaa ctttctggtg gtcaacagca
     3001 gcgtgtggcg attgcccgcg cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc
     3061 gacttccgct cttgacccgg aactgcgcca tgaagtgctg aaggttatgc aggatctggc
     3121 tgaagaaggg atgacgatgg tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc
     3181 ttcgcgcctg atctttatcg acaaaggccg gattgcagaa gatggcgatc cgcaggtgtt
     3241 gatcaagaac ccgccgagcc agcgcttgca ggaatttttg cagcacgtct cttaacttct
     3301 ctgcctccct ttcccccgaa agggaggcac accagattcc tctcatttaa aattgcctct
     3361 cccccagcat ctatacttat ctttttgctc tgttttctca ctggaggagt catgcggtgg
     3421 atcctgttca tcctcttctg cctgctgggc gcacctgccc acgcggtatc catacccggc
     3481 gttacaacca caacgacaac ggactcaacg actgaaccgg ccccggaacc ggatatcgaa
     3541 caaaaaaaag cggcctatgg cgcactggcg gatgtgctgg ataatgacac ctcgcgtaaa
     3601 gagttgatcg accagttgcg caccgtcgcc gcgacgcccc ctgcggaacc ggtaccgaag
     3661 atcgtgccgc cgacgctggt tgaagagcaa accgtgctgc aaaaggtcac cgaagtcagc
     3721 cgccattatg gtgaagccct ttccgcccgc ttcgggcaac tttatcgcaa tatcaccggc
     3781 tccccgcata agccgtttaa tccacaaacc ttcagcaatg cgctgaccca cttttcaatg
     3841 ttagcggtat tagtgtttgg tttttactgg ctgattcgtc tgtgcgcact gccgctgtat
     3901 tgcaaaatgg gccagtgggc gcggcaaaaa aatcgtgagc gcagtaactg gttgcagctt
     3961 ccggcgatga tcatcggggc gtttattatc gacctgctgt tactggcact gacattattt
     4021 gtcggccagg tattaagcga caacctgaat gcgggcagtc gcaccatcgc tttccaacaa
     4081 agtttgtttc tcaacgcctt tgccctgatt gaatttttca aaaccgtact acgcctgatt
     4141 ttttgcccaa acgtagctga actgcgcccg ttcacgattc atgacgagac cgcccgttac
     4201 tggagtcgtc gcctgagctg gttaagcagc ctgattggct atggtctgat tgtggctgtg
     4261 ccgattatct ctaatcaggt gaatgtacag ataggtgcgc tggcgaacgt catcattatg
     4321 ctgtgcatga ccgtctgggc gttgtacctg atttttcgta ataaaaaaga gatcacccag
     4381 catttgctca actttgcgga gcattcgctg gcctttttca gcctgtttat ccgcgccttt
     4441 gcactggtgt ggcactggct ggcaagcgcc tattttatcg tgctgttttt cttttcgttg
     4501 ttcgatccgg gcaacagcct gaaatttatg atgggtgcaa cggtgcgcag cctggcgatt
     4561 attggtatcg cagcatttgt ttccggtatg ttttcccgct ggctggcgaa aaccatcacc
     4621 ctctcgccac atactcagcg aaattatccg gagctgcaaa aacggctgaa tggatggctt
     4681 tcggcggcac tgaaaacagc gcgtattctg acagtctgcg tggcggtaat gctgctgttg
     4741 agcgcatggg gattgttcga tttctggaac tggctgcaaa acggtgcggg gcagaaaacc
     4801 gtagatatcc tgatccgtat cgcactcatt cttttcttct cggcggttgg ctggacggtg
     4861 ctcgccagtt tgatagaaaa ccggctggct tcggatattc atggccgccc gctacccagc
     4921 gcccgtacgc gtaccctgct aacgctgttc cgtaacgcgc tggcggtgat tatcagtacc
     4981 atcaccatca tgattgtgtt gtcggaaatc ggcgtcaata tcgcgccatt gctggcaggt
     5041 gccggtgcat tgggactggc tatctctttt ggttcgcaaa cgctggtgaa agatattatc
     5101 accggggtat ttattcagtt tgaaaacggc atgaacactg gagatttggt gactatcggg
     5161 ccgttgaccg gcactgtgga acggatgtca attcgctccg tgggcgtgcg ccaggatacc
     5221 ggggcgtatc acatcattcc gtggtcttcg ataaccacct ttgctaactt cgtccgcggt
     5281 attggttcgg tagtggcaaa ctacgatgtt gatcgccatg aagatgctga taaagccaat
     5341 caggcactga aagatgcggt agcagaattg atggaaaacg aagaaattcg cgggctggtt
     5401 attggtgaac cgaattttgc cgggattgtc ggcttaagca ataccgcgtt tacactgcgt
     5461 gtttcgttca ccacgctgcc actcaaacag tggacggtac gctttgccct cgacagccag
     5521 gtgaaaaaac atttcgacct ggcgggtgtt cgcgcgccgg tgcagactta tcaggtgctg
     5581 ccagctccgg gcgcggcccc ggctgaaccg ttgccgccgg gggagccaac gctttaacgc
     5641 tggcgattga caaaacggcg gcgctgctcg tcgttcataa aggtccaggc tataaagcgg
     5701 ctctgctttt gcccctgggc catctctttt tttaccacct tcaccgcacc tacgtccgtc
     5761 aaagcacgat acaacggcgg taagttttca ccacgagata ccagcgaggt aaaccacatc
     5821 acctgcttcg cgaagccttt gctctcttca atcatctttt tgataaaagc gacctcaccg
     5881 ccttcacacc acaactcctg ttgctgacca ccaaagttca gtgcatcgtc tttgttcagc
     5941 cccaggttac ggcgtttacg ttcactacct gcccgtgccg cagcggcgga atcgtggaat
     6001 ggcgggttac acaaggtcgc atcgtattgc tcgtttttat ggatgatgcc gttaaaaatc
     6061 gccccactct ctttttgccg acgcagacga atggcgcggt taagccccgg attagcactg
     6121 atgatcgcct gcgcactgct taacgcctgg ctgctggttt cactaccggt aaaacgccag
     6181 ccatattcat gtacgccaat cagcggataa atacagttcg caccaacgcc gatatccaga
     6241 atgctggcat tagctggaat tgttccgctt gcttcggcca gtaaatcggc aaggtgatga
     6301 atataatccg cccggcccgg tactggtggg cagagaaaac cgtccgggat atcccagttc
     6361 gctacggcgt aaaaatgggc cagcaacgcc ttattgagcg ccttcaccgc cagcggattg
     6421 gcaaagtcta cgctttgctc cccggcgggt gtaagcgtga ggaattgcct gagttcagga
     6481 ttgacctgac aaagcgtggc gagatcgtag cggctgtgat gacggttgcg cggatgcaac
     6541 cccggtttct gggcggacat agcattctcc tttaagcagc ggcgtaagat acccgcataa
     6601 cgcgacaggg taaatatctg gctgcgttta tcatttctaa aaaccttcac atggctatgg
     6661 tttatggcaa ttcaccataa aaaacatcat catgcatgtc attgatttgt cttattttgt
     6721 aaaaacacat cgttcaaaaa ataagcgcat ctctgtcaat tttttgcgtc attttcatcg
     6781 cgcaggcgac ctacacttat ctggcactac ccgttgtatt tatgaggatg tcattatgaa
     6841 aaagtgcctc acactactga ttgccaccgt cctgagcgga atctctctca cggcttatgc
     6901 cgcgcaaccg atgagtaacc tggacagtgg tcaactacgg cccgccggta ccgtttcggc
     6961 aactggcgca tcaaacctaa gcgatctgga ggataaactg gcagaaaaag cgcgcgaaca
     7021 aggcgcgaaa ggttatgtca ttaattccgc aggcggaaat gaccagatgt tcggtactgc
     7081 aatcatctac aaataacccc accaacgtaa ctgcattcgc ctctggatgc agttttcgtc
     7141 caatttgtaa gcaaaaaaca tcaaatatga acgcaatgta aataaatgta tttctttttc
     7201 gcgcaatggg tgatagaaaa tcgcgccaag tgataatgct tatcaatatt attatcactt
     7261 gcacgtacgc tatcacggga ttaacagtgg catcgcatcc gcagagatgc tttctcgtga
     7321 cagtgaaaat ttcaccatat aagaaaaagt cacctgcaaa atggaaaaca atcgcaattt
     7381 ccctgccaga caatttcatt cgctcacgtt ctttgccggt ctttgtattg gtattacgcc
     7441 tgtggcacag gcactcgctg ccgaagggca agctaacgcg gatgacacgc tggttgtcga
     7501 agcatcaacg ccttcgcttt atgcgccaca aaaatctgcc gatccgaaat tctcgcgtcc
     7561 ggtagcggat actacccgca cgatgacagt gatttctgaa caagtgatta aagatcaggg
     7621 tgcaaccaac cttaccgacg cgctcaaaaa cgtccccggc gtgggtgcgt tttttgcggg
     7681 tgagaacggt aactccacca ctggcgacgc catttatatg cgcggtgccg atacctctaa
     7741 cagtatttat atcgatggca ttcgcgatat cggcagcgtc tcgcgcgaca ccttcaatac
     7801 cgagcaggtc gaagtaatta aagggccgtc cggcaccgac tacgggcgca gcgcaccgac
     7861 aggctcgatc aatatgatca gcaaacagcc gcgcaatgat tccggcattg acgcctccgc
     7921 cagtattggc agcgcctggt tccgccgcgg cacgctggac gtcaatcagg tcattggtga
     7981 taccactgcg gtgcgcctga atgtaatggg cgaaaaaacg cacgatgccg gacgcgacaa
     8041 agtcaaaaat gagcgttacg gcgtcgcccc ttctgtcgct tttggccttg gtacagcgaa
     8101 tcgtttgtat cttaattatc tgcatgtcac ccagcacaac acgccagacg gcggcattcc
     8161 gaccatcggt ttaccgggtt tttctgcccc atctgcggga acagcggccc tgaatcattc
     8221 cggaaaagtt gatacgcata acttttacgg cacggattcc gattacgacg attcgaccac
     8281 cgacaccgct accatgcgtt ttgagcacga catcaacgat aacaccacca ttcgcaacac
     8341 tacgcgttgg tcgcgcgtaa agcaggatta cctgatgacg gcgattatgg gtggagcgtc
     8401 gaatattacc cagcccacca gcgatgtgaa tagctggacg tggtcacgca cggcgaatac
     8461 caaagatgtg agtaacaaaa ttctcaccaa ccagaccaac ctgacctcga cgttctatac
     8521 cggcgctatc ggtcatgatg tcagtaccgg cgtggaattt acccgcgaaa cacagactaa
     8581 ctatggcgtt aatccggtga cgttaccgac ggtaaatatt tatcatcctg acagcagcat
     8641 tcatcccggc ggcctgacgc gcaacggcgc aaacgccaat ggtcagacgg ataccttcgc
     8701 aatttatgcc ttcgatacgc tgcaaatcac ccgtgatttt gagctgaacg gtgggatccg
     8761 tctggataat tatcatactg aatatgacag tgccaccgcc tgcggcggca gcgggcgcgg
     8821 tgccatcacc tgccctgctg gcgtggcaaa aggttctccg gtaaccaccg tcgacaccgc
     8881 caagtcgggc aatctggtga actggaaagc cggggcgctg tatcacctga cggaaaacgg
     8941 caatgtttat attaactatg ccgtttccca gcagccgccg ggcggcaaca acttcgccct
     9001 tgcgcagtct ggcagcggta acagtgccaa ccgcaccgat tttaaaccgc aaaaagccaa
     9061 caccagcgag attggcacca aatggcaggt tctggataaa cgcctgctac ttaccgccgc
     9121 gctgttccgt actgatattg aaaatgaagt tgagcaaaat gatgatggga cttattcgca
     9181 atatggtaag aaacgtgtcg aaggctatga gatatccgtg gccgggaata tcacccccgc
     9241 gtggcagatg attggcggct atacccagca aaaagcaact atcaaaaacg gcaaagatgt
     9301 tgcccaggat ggttcctcat cgctgccgta taccccggaa cacgccttca ccttatggag
     9361 ccagtatcag gcaaccgatg atatttctgt tggtgcgggc gcacggtata tcggcagtat
     9421 gcataaaggt tcagacggcg cggtgggaac gccagcattt accgaaggtt attgggtcgc
     9481 cgacgccaaa ctggggtatc gggttaatcg caatctcgac ttccagctaa acgtctacaa
     9541 cctgtttgat accgattacg tcgcctcaat caataagagc ggctaccgtt atcacccggg
     9601 cgagccaaga accttcttgc tcacagccaa tatgcatttc tgattcagat gtggggcgca
     9661 ggccccactt tttggagaac ttgtatgatg taccacattc ccggcgtgtt atcgccacag
     9721 gacgtcgccc gttttcgcga acacctggaa caagccgaat gggtggatgg acgcgtcacc
     9781 accggcgcac aaggtgcgca agttaagaac aatcaacaag tagacacccg cagcgccctg
     9841 tacgccgccc tgcaaaatga ggtgctgaac gcggttaacc aacatgcttt gttctttgcc
     9901 gcggccttgc cgcgtaccct ttccacgccg ctgtttaatc gctatcagaa caatgaaacc
     9961 tatggttttc atgtggatgg cgcagtacgc agccatccac aaaacggctg gatgcgcact
    10021 gacctttctg ccacgctgtt tttaagcgat ccagaaagct acgacggcgg cgaactggtc
    10081 gttaatgaca ccttcgggca acatcgggta aaactcccgg caggcgatct ggtgttgtat
    10141 ccctccagca gcctgcattg cgtgacaccc gtaacccgcg gcgtgcgagt ggcatcattt
    10201 atgtggatcc agtcgatgat ccgcgatgat aaaaagcgcg ccatgctgtt tgaactggac
    10261 aacaatattc agtcgctgaa aagccactac ggcgaaagtg aagagatcct gtcgctgctc
    10321 aatctttatc ataatctgct gcgggaatgg tcggagatct gacgcttgaa ctgcccgagt
    10381 tcgtccctat attagctaca cttaactgta caagcattga tatggggagg acgatatggc
    10441 atccggttgg gctaatgatg acgccgtcaa cgaacagatc aacagtacaa ttgaagatgc
    10501 gattgcccgc gctcggggtg aaattccgcg tggcgaaagc ctgtatgaat gtgaagagtg
    10561 cggtgcccct atcccgcagg cccgtcggga agccattcct ggcgtgcgct tatgcattca
    10621 ttgtcagcag gagaaagatt tacaaaaacc agcttataca ggatataatc gcagaggttc
    10681 gaaagacagc cagttacgtt aactacaact ggcgggatgt atagcgaata taatttgtat
    10741 tttgcgtggc attccgtgcc tttaacgcta ccgtgactgc cacgcggtat aaaacaagtc
    10801 cataagtaca acaaataaat ggtttatcag tatgttagat atcaatcaat ttatttgaac
    10861 aaggtggtca attctcttcg attttatctc tcgtaaaaaa acgtgatact catcacatcg
    10921 acgaaacaac gtcacttata caaaaatcac ctgcgagaga ttaattatga aaactatcaa
    10981 tactgttgtt gctgctatgg ctctttcaac tctgtcattt ggcgtgttcg cggcggaacc
    11041 ggtaacggca tcccaggcac agaacatgaa taaaatcggc gtggtttctg ccgatggcgc
    11101 atccaccctc gatgccctgg aagcgaaact ggctgagaaa gccgcggcgg ccggtgccag
    11161 tggatacagc atcacttctg ccaccaacaa caataaatta agcggtactg cggtaattta
    11221 taagtaattc gtaagctgga tcaggcgttt acgctacgtt cagcacaagt atttccctgt
    11281 ctgtttgccg acagacgcat atgctctaac cctcatgatc ctatgttacc cttgtttgcc
    11341 cgtccgccac tggacgggct ttttttagct ggctagctgc tgacagaaag cctgcaacgt
    11401 ctcttccggc ataccaatct gccgcgccgc atcacaaatg gcctgccagc ttcccgcatc
    11461 cagtggaatc ccctgctcct gccgttcgcg acgcgtgttc acttcccact cgcccggtag
    11521 caaaatcggc ttatcgtcat catgcggcga ggctttcacc cactcggcaa aggcttcggt
    11581 ctgcgcacta caatccggcg cgccgaacag ttccgggttg ataatgatag tggtcatgca
    11641 gttaagaatg gcatcgggac tggtttgtaa cgtttcctga tgcgtcgttt taccgccaga
    11701 aagcgccccg ccaagaattt cacacatcgc tgcaagggcg tagcctttat gttcggcaaa
    11761 ggtcaacagc gaacccaacg gcgactcctg cattaccgcc ggatttgtcg tcggcacgcc
    11821 gttaacgtca atcaggcaac ctggcggcac ggggacgcct ttatgccagg cgacgcgggt
    11881 tttgccaaat gcaatggcgc tggtggcgta atcgagcaac agcgggaaat tatctttacg
    11941 agggaaaacc acacagaacg gattggtgcc aaagcggctg tcgcgaccgt ggaacggcgc
    12001 gaccattgga ataccgacca cgctaacaaa gtggatagag acaaaccccg ccgctgcaca
    12061 ctgctccgcc cagtaaccga tacggccgat atgatgcgag ttatgtagcg ccacggcggc
    12121 aatgccgtgc tgatgcgctt tctcaatccc cagcgccatc gcttcgtgtg ccgcgacctg
    12181 accaaatgcg cgatcgccat cgagcgtgac cgccgccccc gcttctttaa cgactttggc
    12241 atgatggtta atttgcagat gcccctgact ccaggagcgc acatagcttg ggatcatgcc
    12301 aataccatgt gaatcatgcc ctgccaggtt tgccgcgatc aaatgatcgg cgactaattt
    12361 cgcttcctgt tcctcactac ccatctgacg aaatacagcc tgaataaaac tgtgcagcgt
    12421 ctgagcatca aagcgatggc cactttccat ggttaactcc tgtttatgtt atgtgtttgt
    12481 tgttttttat gctgcacgcc gggcgcggga aggtcaataa agatgcagga atttcatatt
    12541 gcggtggggt aaaagccgga tgacatggct catccggctg agataaaaat taaaatgcct
    12601 gattaacgcg cgccaggccg tcgctgatag ttgccgcttc acccgtagcc atcaggcagc
    12661 aagccatctg gattttcagc gattcgggaa tcggttcgct gccagccagg caacgctcaa
    12721 tccactgcgc cgtggtttcc ggatcttttg cttgtggcag taactcgcta ccagcagtgt
    12781 cctgtttttc atacagtacc cgcatccctt cacggtcaat gagattgatc tgcgggcagc
    12841 gctgcggatt agcatacact tcaccttcag taccatgcat aagtagcgcc cgcccgccga
    12901 tatcgctaaa gaacttcgcg acgcgtccaa tgtattccgg atgcgaaacg ctggaaagac
    12961 gcagtgcctc accttcggca aatggcgtcg ccagtttcgc cagggtatgt gcactgttac
    13021 gcacgcccat ccgccagcgc atcgccagtt gtttttccag cggcgggcaa aacgcgccga
    13081 caggcataaa gacaggttga tgttcgtcga gcttcgcctg cgcctgcccg ccgtgcaacg
    13141 ttggcgtaat acccatcagt tcgaaaatgg tttcggtcag cacgcgggtt ggatcttcgc
    13201 taaccccgtg aaccaccaca ggaaaaccga gtttgtgcag cagaatcgcc agcaacggcg
    13261 tcaggttggc ctgtttacgc gcgccgttgt aactggggat gacaatcggc atcggcttgc
    13321 ctgcaggcgg cgtcagcttg atggtgtgat tttgcatggc ttcgtaaaag ccgagcatct
    13381 ctgcttcccc ttcccctttg atacgtagcg caatcaatac gccgcccaac tcaaggtcag
    13441 ggacttcacc attgagcata tgggcataca gaccgcgcgc agtatcccgg tctaaatcgc
    13501 gcgcgtggtt tttcccgcgc ccgatctctt taatgatttt gcgatagtcc atttacgact
    13561 ccttacctga ctcacatcat taacgccgcc gacggcgtgg ggatttcgtt ttttctttct
    13621 ttttaactat accttcaggg acttccggtt gctctatcgg aaataccggt aatgcatcca
    13681 gtagtcgctt gccataattt tttgtcagca agcgtttgtc gtagataacc acttcccccc
    13741 agcaaccgtg gcttcgaatc agtcgcccaa cctgctgaat caggttaaac gaagcgctcg
    13801 gcaggctttg cacctcaaac ggatagcggt taaggctttt cagccattca ccttcggtga
    13861 tcaccaccgg actgtcgatg ggcggaaaag cgattttgtg gatatgcacc tggctgagca
    13921 gagcgccttt cagatcaaga ccttcggcaa aggactgtaa gcccaccagc acgctacgct
    13981 caccgttggc aacgcgtttg cggtgcagtt caactaagcg ataacgtggc tgatcgccct
    14041 gcaccagcaa catcaggcgt aaatccgtca catagtcgag aaagcgctgc atcgcacgcc
    14101 cgctggcaaa cagcaccaac ataccgagat gttttttgct ctccacctgc tcacggaaaa
    14161 aggccgccat ttcagcaatg tgctgttctt cgttgtcgat ggaaggctca acgcgcatcc
    14221 ggggaataac aattttgccc tgttcgcagt ggttaaaggg cgaatccagc gccacaaagc
    14281 ggtcacccgc tttctctttc agcccactca tctcctgcaa acgcgaaaaa ctgttcagcg
    14341 aacgcaaggt tgcggaggta acaataatgt gcggaatact gcgccacagc agcctttcca
    14401 gttggtcgct aacacgaata cccacacagt gaaaccacag atgtagctgc ccttcgcgct
    14461 cttcccgcgt cgcccatttg gtcaccggcg caccggaaga ttgcgccaga gacgccagcc
    14521 gccagagttt gctttgcgcc tcgaacatcc ccaacgcgcg gttcatctgc aaaatcaacc
    14581 gatgcaggcg tacaatgtca tggctgccgg ttttctcact taaatcgtta agaaataact
    14641 ccgccagacc acgcagcatc tcggtgagtt ttgccagccg ctggcagatc tccagcactt
    14701 catcaggcag ttcgcccatc gcaaaacggt gctctgcctc ctgcccggca ggcatgtaga
    14761 gattgagaat gttgtttaac gaggcgataa gctcatacag ctcttcacaa tgcgcattca
    14821 aacgttcagg gatcgccagc ggtgggatgg tcttcgggcg aaactgctcc atgcaggtgg
    14881 cgaccagttt ggtgaacagg tccagttgga gccggtacca gggcgcagtg atttcggcgc
    14941 tcatctccag cgcatcccgc gccacatccg gcaggtgatg accttcgtcc agcaccagca
    15001 gtaaattttt cgggtcaggc aataccgctt cgctttccat tgccgccatc accagcgcat
    15061 ggtttgccac caccacttcc gcttcctgaa tttcccgacg agcgacaaaa aacgggcatt
    15121 cgcgatagta gtaacagttg cggttgaggc agctggcttt gtcggtactc aaacgacgcc
    15181 agagatcatc atctatagcg atatcagtat gatcgcgcag gccatcccat ttataagtgt
    15241 cgagatcgcc cttcagcttc gcacaacgtt tttgctcttc ctgattgttc ggcgtcagtt
    15301 cgtcgtcaag aaacgccagc agatcctgtt gcgtgggctc agtgctggcg agcgctgtca
    15361 gattacgcgg gcaaacgtag cgcccacgcc caaaagcggc agtgaattta agatcgggaa
    15421 tgatcttttt cagcagcggt aaatctttgc tgtagatctg atcctgcaat gccacgttgg
    15481 cggtacttac caccagcgtt ttttgctctt cgcgggcaat ggcgatgccg gggatcaaat
    15541 aggagagcgt tttcccaacg ccggtaggag cttcaatcgc cagatgccgc ccttcttctc
    15601 cggccagcgt tttggcgacg tccgcaatca tctgccgctg cggcgcacgg ggaataaagt
    15661 cggggatctg ttcctgaagc gccttatacc aggcggcaat ttgcgcttta agcgcggcgg
    15721 ttaatgccat gagaaaacct gaaatactgt ataaacagcc aatattgtgg catttttcgg
    15781 ttttcagggg taatggtttt tctgtttctg gaaagcggct cgcaataacc agcaggatga
    15841 tgcgtaaagc gcttcgcgct aaattcgaac aacatgcaga actgcgtaca ctcttgctgg
    15901 caaccgcgtc cgcaaaactg gttgagcata cgcaaaatga tgcttactgg ggagatggcg
    15961 gtaatggtca gggcaagaat cgcctggggt accttttaat ggcgttgcgc ggacaattgg
    16021 ctgcggagaa gtaacgcatc gggtggcttg tgggcgttaa aacagagaaa tctttttgcc
    16081 gcgctcgcaa atggctgaca agtgcttgtt gtgaaatgcc ggatgcggcg cgagcgcctt
    16141 atccggccta caaaaacatg cacattcaat atattgcaga gttcacgtag gcctgataag
    16201 cgtagcgtat caggcagttt tgtgtttgtc atcagtctca tgccgggcat agcccggcat
    16261 aaaagattac tgcgcagcgg caggtttacg cggacggcgg cgacgttgtt gttcgcctgc
    16321 cggtttggca tcgccgagac gacgagacgg tttttctgcg ggcttcgcgt taccagactt
    16381 cgcaccactt tccgcacggc gtggttgctg ttgaccgcga ccaccgccct gtccacgacc
    16441 gccgccacgt tgctggcgac cgttttggat cggttcggct ttgattgacg ggtccggctc
    16501 atagcccaga atcgcaatgc gcgggatctc ttttttcagc agtttttcga tatcacgcag
    16561 cagtttgtgt tcatcaacac acaccagcga caatgcttcg ccagttgccg ccgcacggcc
    16621 agtacgcccg atacggtgaa cataatcttc cggtacgttt ggtaactcat agttgacgac
    16681 gtgcggcaac tcttcgatat ccagaccacg cgcagcgatg tcagttgcca ccagtacacg
    16741 aatatcgccc gatttaaaat cggccagcgc acgagtacgc gcaccttgcg atttattacc
    16801 gtggatcgcc gcactacgga tgccatcttt attaagctgt tcagccagat ggttagcgcc
    16861 atgtttggta cgggtaaaca ccagcacctg ctgccagttc cctttcccaa tcatatgcga
    16921 cagcaattcg cgtttgcgtt tcttatcgac aaagtgaacg tgctgggtca cctgatcaga
    16981 cgcagtattg cggcgtgcca cttcgatttc cagcgggttg tgcaacagtt tttccgccag
    17041 ggctttaata tcgtcagaga aggtcgcgga gaacaacagg ttctggcgct tcgctggcag
    17101 ttttgttaac acgcgacgga tatcgtggat aaagcccatg tcgagcatgc ggtcagcttc
    17161 atcaaggacg aggatttcaa cctgatccag cttcactgca ttttgatgtt ccaggtccag
    17221 cagacgtccc ggcgttgcca ccagcacatc aacgccgccg cgcagcttca tcatctgcgg
    17281 gttaatgctg acaccgccaa acaccaccag cgaacgaatg ttcaggtatt tgctgtaatc
    17341 acggacgttt tcgccaatct gcgccgccag ttcacgggtc ggggtaagaa tgagcgcacg
    17401 caccggacga cgccctttgg cgtgcggctg gcgagtgatc aggtgttgca acagcggcag
    17461 cgtaaagccc gctgttttac ctgtaccggt ctgggcgctg gccatcaggt cgcggccttc
    17521 cagcaccgca gggatcgcct gctgctgaat aggggtgggt tcacggtaac cctgctcggc
    17581 aacggcgcgc aggatatcag ggcttaaacc caaagaatcg aaagacataa ctactccgaa
    17641 ccgccccgac cgttaccggt gtagttttca gggagatact gaaaaggaaa atgacaaaaa
    17701 ccacaatgtc atgagggagc ggagtgtagc agtttttgtg acgcagcgca taaattatcc
    17761 ctgtgactgg cgtagctaaa attcttagcc atactggaca actcccctat ggagtcataa
    17821 tcttaatcaa tcatttgatt aagaatggac tgtgcgatga ataatcctgc catgacaatc
    17881 aagggtgaac aggcgaaaaa acagctgatt gctgcggcac tggcacagtt tggtgaatat
    17941 ggaatgaacg ccaccacccg cgagatagcc gcccaggccg ggcagaatat cgctgccatc
    18001 acctactact tcggttcgaa agaagatttg tacctcgcct gcgcccagtg gattgctgat
    18061 tttattggcg agcagttccg tccgcacgcc gaagaagccg aacgtttgtt cgcgcaacca
    18121 cagccggatc gggctgccat ccgtgaactg atccttcgcg cctgcagaaa catgattaag
    18181 ctgctcaccc aggatgacac cgtcaacctc agcaaattta tctcccgtga gcagctctct
    18241 cccacggcag cctaccacct ggtgcacgaa caggtgatta gtccgctaca cagtcacctt
    18301 acacgcctga ttgccgcctg gaccggctgc gacgctagcg atacccgcat gatccttcat
    18361 acccatgcgt tgattggtga gattctggcg tttcgtcttg gcaaagaaac tattctgtta
    18421 cgtaccggct ggaccgcgtt cgatgaagaa aagaccgaac tgatcaacca gacggtgacc
    18481 tgtcatattg acctgatttt gcaaggatta tcgcaaagga gtttgtagtg atgaaaaaac
    18541 ctgtcgtgat cggactggcg gtagtagtac ttgccgccgt ggttgccgga ggctactggt
    18601 ggtatcaaag ccgccaggat aacggcctga cgctgtatgg caacgtggat attcgtacgg
    18661 tgaatcttag tttccgtgtt ggggggcgcg ttgagtcgct ggcggtggac gaaggtgatg
    18721 cgatcaaagc gggccaggtg ctgggcgaac tggatcacaa gccgtatgag attgccctga
    18781 tgcaggcgaa agcgggtgtt tcggtggctc aggcgcagta tgacctgatg cttgccgggt
    18841 atcgagatga agaaatcgct caggccgccg cagcggtgaa acaggcgcaa gctgcctatg
    18901 actatgcgca gaacttctat aaccgccagc aaggactgtg gaaaagccgc accatttcgg
    18961 caaatgacct agaaaatgcc cgctcctcgc gcgaccaggc gcaggccacg ctgaaatcag
    19021 cgcaggataa attgcgtcag taccgttccg gtaaccgcga acaggacatc gcccaggcga
    19081 aagccagcct cgaacaagca caggcgcaac tggcgcaggc ggagctgaat ttgcaggact
    19141 caacgttgat agcaccgtct gatggcacgc tgttaacgcg cgctgtagag ccaggcacgg
    19201 tgcttaatga aggtggcacg gtatttaccg tctcgctgac gcgtccggtg tgggtacgcg
    19261 cctatgttga tgaacgtaat cttgaccagg cccagccggg gcgcaaagtg ttgctttata
    19321 ccgatggtcg cccggacaag ccgtatcacg ggcagattgg tttcgtttcg ccgactgctg
    19381 aatttacccc gaaaaccgtc gaaacaccgg atctgcgtac cgacctcgtc tatcgcctgc
    19441 gtattgtggt gaccgacgcc gatgatgcgt tacgccaggg aatgccggta acggtgcaat
    19501 tcggtgacga ggcaggacat gaatgatgcc gttatcacgc tgaacggcct ggaaaaacgc
    19561 tttccgggca tggacaagcc cgccgtcgca ccgctcgact gcaccattca cgccggttat
    19621 gtgacggggc tggttgggcc ggacggtgcg ggcaaaacca cgctgatgcg gatgctggcg
    19681 ggattactga aacccgacag cggcagtgcc acggtgattg gctttgatcc gatcaaaaac
    19741 gacggcgcgc tgcacgccgt gctcggctat atgccgcaga aattcggcct gtatgaagat
    19801 ctgacggtga tggagaacct caatctgtac gcggatttgc gcagcgtcac cggcgaggca
    19861 cggaagcaaa cttttgctcg cctgctggag tttacgtctc ttggcccgtt taccggacgc
    19921 ctggcgggca agctctccgg tgggatgaaa caaaaacttg gcctggcctg taccctggtg
    19981 ggcgaaccga aagtgttgct gctcgatgaa cccggcgtcg gcgttgaccc tatctcacgg
    20041 cgcgaactgt ggcagatggt gcatgagctg gcgggcgaag ggatgttaat cctctggagt
    20101 acctcgtatc tcgacgaagc cgagcagtgc cgtgacgtgt tactgatgaa cgaaggcgag
    20161 ctgctgtatc agggagaacc gacggccctg actcaaacca tggccggacg cagctttctg
    20221 atgaccagcc cgcacgaggg caaccgcaaa ctgttgcaac gggcattgaa actgccgcag
    20281 gtcagcgacg gcatgattca ggggaaatcg gtacgtctga tcctcaaaaa agagaccaca
    20341 ccagacgata ttcgccatgc cgacgggatg ccggaaatca acattaacga aactacgcca
    20401 cgttttgaag atgcgtttat tgatttgctg ggcggtgccg gaacctcgga atcgccgctg
    20461 ggcgcaatat tgcatacggt ggaaggtaca cctggcgaga cggtgatcga agcgaaagag
    20521 ctgaccaaaa agtttggcga ttttgccgcc accgatcacg tcaactttgc cgtcaaacgc
    20581 ggtgaaatct ttggtttgct cgggccaaac ggcgcaggga aatcgaccac ctttaagatg
    20641 atgtgcggtt tgctggtacc gacctctggt caggcgctgg tgctgggcat ggatctgaaa
    20701 gagagttccg gtaaagcgcg ccagcatctc ggctatatgg cgcaaaaatt ttcgctctac
    20761 ggcaacctga cggtcgaaca gaatttacgc tttttctctg gtgtgtatgg cttacgcggt
    20821 cgggcgcaga acgaaaaaat ctcccgtatg agcgaggcgt tcggcctgaa aagtatcgcc
    20881 tcccacgcga ccgatgaact gccattaggt tttaaacagc ggctggcgct ggcctgttcg
    20941 ctgatgcatg aaccggacat tctgtttctc gacgaaccga cgtccggcgt tgatcccctc
    21001 acccgccgtg aattttggct acatatcaac agtatggtag agaaaggcgt cacggtgatg
    21061 gtcaccaccc actttatgga tgaagcggaa tattgcgacc gcatcggcct ggtgtaccgc
    21121 gggaaattaa tcgccagcgg cacaccggac gatttgaaag cgcagtcggc caacgatgag
    21181 caacccgatc ccacgatgga gcaagccttt attcagttga tccacgactg ggataaggag
    21241 catagcaatg agtaatccga tcctgtcctg gcgtcgcgtg cgggcgctgt gcgtcaaaga
    21301 gacgcggcag atcgttcgcg atccgagtag ctggctgatt gcagtagtga tcccactgct
    21361 actgctgttt atttttggat acggcattaa cctcgactcc agcaagctgc gcgtcgggat
    21421 tttactggaa caacgtagcg aagcggcgct ggatttcacc cacaccatga ccggttcgcc
    21481 ctacatcgac gccaccatca gcgataaccg tcaggaactg atcgccaaaa tgcaggcggg
    21541 gaaaattcgc ggtctggtgg ttattccggt ggatttcgcc gaacagatgg agcgcgccaa
    21601 cgccatcgca ccgattcagg tgattaccga cggcagtgag ccgaataccg ctaactttgt
    21661 gcaggggtat gtcgaaggga tctggcagat ctggcaaatg cagcgagcgg aggataacgg
    21721 gcagactttt gaaccgctta ttgatgtaca aacccgctac tggtttaacc cggcggcgat
    21781 aagccagcac tttattatcc ccggtgcggt gactattatc atgacggtca tcggtgcgat
    21841 tctcacctcg ctggtggtgg cgcgagaatg ggaacgcggc accatggagg ctttgctctc
    21901 tacggagatt acccgcacgg agctgctgct gtgtaagctg atcccttatt actttctcgg
    21961 gatgctggcg atgttgctgt gtatgctggt gtcggtgttt attctcggcg tgccgtatcg
    22021 cgggtcgctg ctgattctgt tttttatctc cagcctgttt ttactcagta ccctggggat
    22081 ggggctgctg atttccacga ttacccgcaa ccagttcaac gccgctcagg tggccctgaa
    22141 cgcggctttt ctgccgtcga ttatgctttc cggctttata tttcagatag acagtatgcc
    22201 cgcggtgatc cgcgcggtga cgtacattat tcccgcacgt tatttcgtca gcactctgca
    22261 aagcctgttc ctcgccggga atattccagt ggtgctgata gtgaacgtgc tgtttttgat
    22321 cgcttcggcg gtgatgttta tcggcctgac gtggctgaaa accaaacgtc ggctggatta
    22381 gggagaagag catgtttcat cgcttatgga cgttaatccg caaagagttg cagtcgctgc
    22441 tgcgcgaacc gcaaacccgc gcaattctga ttttacccgt gctgattcag gtgatcctgt
    22501 tcccgttcgc cgccacgctg gaagtgacca acgccaccat cgccatctac gatgaagata
    22561 acggcgagca ctcagtagaa cttactcaac gttttgcccg cgccagcgcc tttacccatg
    22621 tgctgctgct gaaaagcccg caggagatcc gcccgaccat cgacacacaa aaggcgttac
    22681 tactggtgcg tttcccggct gacttctcgc gcaaactgga taccttccag accgcgccgt
    22741 tgcagttgat cctcgacggg cgtaactcca acagtgcgca aattgccgcc aactacctgc
    22801 aacagatcgt caaaaattat cagcaggagc tgctggaagg aaaaccgaaa cccaacaaca
    22861 gcgagctggt ggtacgcaac tggtataacc cgaatctcga ctacaaatgg tttgtggtgc
    22921 cgtcgctgat cgccatgatc accactatcg gcgtaatgat cgtcacttca ctttccgtcg
    22981 cccgcgaacg tgaacaaggt acgctcgatc agttgctggt ttcgcccctc accacctggc
    23041 agatattcat cggcaaagcc gtaccggcat taattgtcgc caccttccag gccaccattg
    23101 tgctggcgat tggtatctgg gcgtatcaaa tccccttcgc cggatcgctg gcgctgttct
    23161 actttacgat ggtgatttac ggtttatcgc tggtgggatt cggtctgttg atttcatcac
    23221 tctgttcaac acaacagcag gcgtttatcg gcgtgtttgt ctttatgatg cccgccattc
    23281 tcctttccgg ttacgtttcg ccggtggaaa acatgccagt gtggctgcaa aatctgacgt
    23341 ggattaaccc tattcgccac tttacggaca ttaccaagca gatttatttg aaggatgcaa
    23401 gtctggatat tgtgtggaat agtttgtggc cgctactggt gataacggcc acgacagggt
    23461 cagcggcgta cgcgatgttt agacgtaagg tgatgtaact tcttatcttt cgccagcaaa
    23521 gacaccaccg ccgggccggc aaggattgcc agccctgcaa ttgccagcaa aaagttgttt
    23581 tccagtacgc gggacaacag ccacagcaca atcagcgctg caccaaaata ccaggtcgtg
    23641 gtcagctctt ccagcacgtc tgccacctca cgccagcgtt gctctgggtg gcgctgaaac
    23701 accagcatca tcactaccgt gacacaatac agtgcgcata atcctacgcc gacgtgaacc
    23761 agagttttac tcatccaacc caccaccagc agcgccacta ccagtaaatg caacataatc
    23821 tgccagcaac ttaggccagt tgcgacacga acacgttgtt gccacttcat ggcttctctc
    23881 ctgaagaatt tatctctact tattcagagt accgaacttt acggaaaatt ccgtaagacc
    23941 gcaccttttt gtgacgccga ctacactatt tcaatgagat aagaggcaaa gacaggaaaa
    24001 ttatgcccga tcaaacacaa caattttcgt tcaaggtgct caccatcaat attcacaaag
    24061 gctttaccgc gtttaaccga cgcttcattt taccggaact tcgcgacgcc gtgcgtaccg
    24121 tcagcgccga tattgtttgc ctgcaggaag tgatgggcgc gcacgaagtt catccgctgc
    24181 atgtggaaaa ctggcccgat acctcgcact acgagtttct cgccgacacc atgtggagcg
    24241 atttcgccta cggtcgcaat gccgtatacc cggaaggaca tcacggtaac gccgttcttt
    24301 cacgttatcc cattgaacat tatgagaatc gtgatgtttc ggtcgatggt gcggaaaagc
    24361 gcggcgtgct ctactgccgc attgtgccgc cgatgaccgg gaaagcgatt catgtgatgt
    24421 gcgtacatct gggcctacgt gaggcgcacc gtcaggcgca gcttgcgatg ctcgccgaat
    24481 gggtgaatga gctaccggac ggcgaatcgg tattggtagc gggtgatttc aacgactggc
    24541 ggcaaaaagc taatcatccg ttaaaagtgc tggccgggct ggatgagatt tttacccgcg
    24601 cccacggacg tccggcgcgc acgtttccgg tgcaatttcc tctactacga ctggacagga
    24661 tctacgtcaa aaatgccagc gccagcgcgc caaccgcgtt gccgctgcgg acatggcgac
    24721 acctttctga tcatgcccct ttaagtgcgg agattcattt atgaaatgta gctggcgcga
    24781 aggcaatatg atccagttgc tggaaaacgg cgagcaatat tatcccgcgg tgtttaaggc
    24841 gattggcgag gcgcaagaac gcatcattct tgaaacgttt atctggtttg aggatgacgt
    24901 cggcaaacag ctgcatgcgg cgctattggc agcagcgcaa cgcggggtaa aagcggaagt
    24961 cttgctggat ggctacggtt cgccggatct cagcgatgaa tttgtcaatg aactgacggc
    25021 agctggcgtg gtgttccgct actacgatcc ccgccctcgc ctttttggta tgcgcaccaa
    25081 tgtgtttcgc cggatgcatc gcaaaattgt ggtgatcgac gcgcgtatcg cctttattgg
    25141 cgggctgaat tactccgccg agcatatgtc cagctacggc ccggaggcca aacaggatta
    25201 cgcggtacgc ctcgaagggc cgattgtcga agatattctc cagtttgagc tggaaaacct
    25261 gccaggacaa agcacggcgc gacgctggtg gcgacgccat cataaagcgg aagagaaccg
    25321 ccagccggga gaagcgcagg tattgctggt ctggcgcgat aacgaagaac atcgcgatga
    25381 tatcgaacgt cactatctga aaatgctcac tcaggcgcgg cgggaagtga ttatcgccaa
    25441 cgcctacttc ttccccggct atcgtttttt acacgccttg cgtaaagcgg cacgacgcgg
    25501 ggtgcggatc aaactgatca ttcagggcga accggatatg ccgattgtca gagtcggcgc
    25561 gcgtttgctg tataactatc tggttaaagg tggcgttcag gtgtttgagt accgccgccg
    25621 cccgctccac ggcaaagtgg cattgatgga cgatcactgg gcgacagtag ggtccagtaa
    25681 tctcgatccg ctcagtttgt cactgaatct cgaagcaaat gtcatcatcc acgatcgtaa
    25741 ttttaaccag acgctgcgcg ataatctgaa cggcattatc gccgcagatt gtcagcaggt
    25801 cgatgagacc atgctgccga aacgcacctg gtggaacctg actaaaagcg tgctggcgtt
    25861 ccactttcta cgccacttcc cggcgctggt gggctggctt ccggcgcaca cgccacgtct
    25921 ggcgcaggtt gatccccccg cacaaccgac aatggaaacg caggatcggg tagaaactga
    25981 aaacacgggg gtaaaaccct gatgagtaaa tcacacccgc gctggcgctt agcaaagaag
    26041 atcctcacct ggctgttttt tatcgcggtg attgtgttgt tggtggtcta cgccaaaaaa
    26101 gtggactggg aagaggtctg gaaggtcatc cgagactaca atcgcgttgc actgctcagt
    26161 gcggtcgggc tggtggtcgt cagctatctg atttacggtt gctacgacct gctcgcccgt
    26221 ttctactgcg gtcacaaact ggcgaagcgc caggtgatgc tggtgtcgtt tatctgctac
    26281 gccttcaacc tgacactcag tacctgggtc ggcggcattg gtatgcgcta tcgtttgtac
    26341 tcccgactgg ggttaccggg cagcactatt acgcggattt tttcgctcag tattaccacc
    26401 aactggctag gctatatttt gctagcgggg gttatcttta ccgcaggcgt ggtggagttg
    26461 ccggatcact ggtatgtcga tcaaactacg ctgcgtattc tcggcattgg cttactgatg
    26521 attatcgcgg tttatttgtg gttttgcgct ttcgcgaagc accgccatat gaccatcaaa
    26581 gggcaaaagc tggtgctgcc ttcgtggaaa ttcgccctcg cccagatgct gatttccagc
    26641 gttaactgga tggtaatggg agcgattatc tggctgttac ttggtcaaag cgtaagctat
    26701 ttctttgtac tgggcgtgtt gctggttagt agtattgctg gcgtcatcgt gcatattccg
    26761 gcgggaattg gtgtgctgga agcggtgttt atcgcgctac tggctgggga gcatacctcc
    26821 aaaggcacaa ttatcgccgc cctacttgct taccgtgtgc tgtattactt tatcccactg
    26881 ctgctggcgc tgatctgtta cctgttgctg gaaagccagg cgaagaagct acgggcgaaa
    26941 aatgaagcgg cgatgtgaag tatagttaag ttctttagta tgtgcattta cggctaatga
    27001 aaaaaccgcg tatgcctttg ccagacaagc gttatagcta tagcagcaag aatcccaaag
    27061 aaaccgataa aatagtaata gagtgttaat gcacctataa aagcacaacg aaccggactc
    27121 cataattctt cactatggct ttttcctgtc agttcaagga gcttatttcg tccatgcgag
    27181 ataatgcctg accataagac aatcattaag caacttatta cccaaatggg tcgttcactc
    27241 attaatgtgg cattcacgat gattaccagg gccaaccctg tcaacgtcat cataatgata
    27301 aaacgatgag aaccaggatc aaatttaaac aatagacctg ccagcatact gattaaaaac
    27361 atcgatcccg cagtgctgaa cacgacgata atggcagata caggcgctaa actcgcaacg
    27421 acaatcgcca ccaaagagat taaaaacaaa tatattacgc ccgcgatggc ggtatttatc
    27481 ttgttgatat ctttacttaa gccatacata gaaagtaata ataatcctat agcaacatac
    27541 atttgcggtg aatcatattt tgttgccgta atttcgagat acttagcgcc ccaggccagc
    27601 cctgcggttg ccaccaaacc caaagcggta atgagccata tgccgtttaa tatcctggct
    27661 tcgtgctgaa aatctaattt attgctattt tgcgagtatg attccattct tttagttgtc
    27721 cttagaaaaa acattaatat tcaccatcaa
//
LOCUS       DACADF010000058        27643 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.058, whole genome shotgun sequence.
ACCESSION   DACADF010000058 DACADF010000000
VERSION     DACADF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.058"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(27..1706)
                     /gene="bcsG"
                     /locus_tag="ISB30_002291"
     CDS             complement(27..1706)
                     /gene="bcsG"
                     /locus_tag="ISB30_002291"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAP1989920.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPHYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPIVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1703..1894)
                     /gene="bcsF"
                     /locus_tag="ISB30_002292"
     CDS             complement(1703..1894)
                     /gene="bcsF"
                     /locus_tag="ISB30_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAP1989921.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1891..3462)
                     /gene="bcsE"
                     /locus_tag="ISB30_002293"
     CDS             complement(1891..3462)
                     /gene="bcsE"
                     /locus_tag="ISB30_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAP1989922.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3735..3923
                     /locus_tag="ISB30_002294"
     CDS             3735..3923
                     /locus_tag="ISB30_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989923.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            3935..4687
                     /gene="bcsQ"
                     /locus_tag="ISB30_002295"
     CDS             3935..4687
                     /gene="bcsQ"
                     /locus_tag="ISB30_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAP1989924.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDASPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4684..7302
                     /gene="bcsA"
                     /locus_tag="ISB30_002296"
     CDS             4684..7302
                     /gene="bcsA"
                     /locus_tag="ISB30_002296"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HAP1989925.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGTRRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYIYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            7313..9652
                     /gene="bcsB"
                     /locus_tag="ISB30_002297"
     CDS             7313..9652
                     /gene="bcsB"
                     /locus_tag="ISB30_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAP1989926.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9659..10765
                     /gene="bcsZ"
                     /locus_tag="ISB30_002298"
     CDS             9659..10765
                     /gene="bcsZ"
                     /locus_tag="ISB30_002298"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAP1989927.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKENLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10747..14220
                     /gene="bcsC"
                     /locus_tag="ISB30_002299"
     CDS             10747..14220
                     /gene="bcsC"
                     /locus_tag="ISB30_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HAP1989928.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEALLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPMQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            14302..16290
                     /gene="hmsP"
                     /locus_tag="ISB30_002300"
     CDS             14302..16290
                     /gene="hmsP"
                     /locus_tag="ISB30_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAP1989929.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQDLVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16473..17759
                     /locus_tag="ISB30_002301"
     CDS             16473..17759
                     /locus_tag="ISB30_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAP1989930.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            17979..19475
                     /locus_tag="ISB30_002302"
     CDS             17979..19475
                     /locus_tag="ISB30_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP1989931.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(19571..20500)
                     /locus_tag="ISB30_002303"
     CDS             complement(19571..20500)
                     /locus_tag="ISB30_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1989932.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSATICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            20732..21499
                     /gene="pdeH"
                     /locus_tag="ISB30_002304"
     CDS             20732..21499
                     /gene="pdeH"
                     /locus_tag="ISB30_002304"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAP1989933.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEELPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            21569..23629
                     /locus_tag="ISB30_002305"
     CDS             21569..23629
                     /locus_tag="ISB30_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAP1989934.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLSFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(23808..25130)
                     /locus_tag="ISB30_002306"
     CDS             complement(23808..25130)
                     /locus_tag="ISB30_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAP1989935.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFTPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(25523..26536)
                     /gene="yhjD"
                     /locus_tag="ISB30_002307"
     CDS             complement(25523..26536)
                     /gene="yhjD"
                     /locus_tag="ISB30_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAP1989936.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGVAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(26584..27555)
                     /locus_tag="ISB30_002308"
     CDS             complement(26584..27555)
                     /locus_tag="ISB30_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1989937.1"
                     /translation="MLEKTINNAICTLLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKSLLLPRLSEFLYLHPGIELELSSHDRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGSMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEYLD"
BASE COUNT         6566 a         7021 c         7638 g         6418 t
ORIGIN      
        1 gggaaactct gcggcctttt tcgttcttac tgcgggtaag gcacccagtc gccgccgttc
       61 aggcgaacgt acggtttatc ctggtattga ataactactg catttgagtt ctcggagacc
      121 ggtgctgttt gtggtaaccc actggtgagt tttttccagt caacattgtc ttcggtgaaa
      181 atcttgccat caagaacgcg aaccaccaga tcggagatag ccaggaagct gctcggttgg
      241 tcgatgacaa tcggagcccc ctgatgtggt gccttcatgc cgaagaattt caccccaacg
      301 gggacgtctg tgatagacgg gctagggata tcacgtagac cagatacctg cattctgtcg
      361 cccttcagcg cgccgccgtg ttccggcacc acgaccacca tcactttacg acccgatttc
      421 tccagttcag taaagaaggc gtccagttca tcaaagaatt tttgcgcccg cgctttgtaa
      481 tccgctgttt tgctgacgcc cggataatgg ttgccgtcat gcagtggaag cgtgttgtag
      541 aacgtggcac tacggctgtt tttatctttt tcggtaacgt ccagccaacg gttaagcacg
      601 gcggtatcgt cataaaccgg cgaaccatca aagcccagca aaataaccgg cagatttgtt
      661 tgatccatca attcgctctg catgccgcca ttttcgcgaa cttccttcaa aaaaccaccg
      721 aactggccgt tatgtcccat catcaggtgc tgggtaaagc ccagtttcga caggttatca
      781 aacagatagc agtcgttatt tgccggttga tacagattag tgtgcgaagt ctgcccgcag
      841 ctggcgcgca gtaaacggat cgccgccggg ccactgtagg aggtggcgga gttaaagttc
      901 ttgaactcaa tatcgaaatg cgaccacagt ggatgcgaca tcaatccggc ggcttcaata
      961 tccgaccagg aaagcgaaca gatattaatc accagtagtt caaatggctg agcatcagcg
     1021 ggcagcgaag acgggaagat cgatttacgt ttcgcctccg cgttatagaa attattcagc
     1081 caggcgttaa ggtttgctgt tgttggcggt gcagtttgtg cgggcatatc acccactatc
     1141 ggtgcgccac ccgtcgccgc aacggttgcc gctgcgttac cacccgtcgt tgttacagtg
     1201 gtcgttggtt gaccggctgg ccacaaggag aaacttggtc ccgccagggt aagtacgttc
     1261 agccatagca gaatggcaac cacaaaaacg gtaatgcgaa tccattgtga caggaataac
     1321 caggccacga ataaaacaaa aatggcccca atcatctgcc agttaataaa gcgtgtgaca
     1381 agatcgatta aataatcggt actgaacccc gccacctgcg aaccctggct cattatgctt
     1441 tccggaccag gcaaccaggt gtcatgccag aacaaagcaa agccgatcgg cagggcaatc
     1501 cagtggcgca agcgatgcag gctatagtgc ggaatgggca tcagtagaaa cgcggcaaac
     1561 accaaattga ggagcggatg gaagttaaga tatcccgccc acaacaggcc gaacttaacc
     1621 agaaaataga agttccagcc ggaaaggccg cgccagtatt gccagaggga agaaggcatg
     1681 gcggtatttt gcgtaaattg agtcattttt tggttgccct ggctttttcc gtgcggcgta
     1741 acgtcccggc cggtttaaca taacgaggtt tagcaaagaa caaacgtaag gtgtcgcgaa
     1801 tgcgtcgcaa agagtgccgc gccagatagc ccagcgggaa aaatatcagt gcgcaaacga
     1861 caataatttc aatgatatcg ctgatggtca tcatgatgag cgctccacag catcatctaa
     1921 cagtcgcatt ggttctggta ttcgtcgcca gtggcgacca tcatgctcgg cattgatgac
     1981 cggtttagaa ctttgcgcta aaggcagcgg catgccccat tgttctgggg caagcaggcg
     2041 catctgcacc agctcggcac tgatttgatc atcttcaaac cagaccattc ggtttgagaa
     2101 aatgtcgcca gtaggcaatg ggaaaatatg attcaacgcg gtatccagat cgttaatccg
     2161 acagaatgag agaaacagca ccagccgatt accgccaatg gtcatgatat cgccggtacg
     2221 gttagggcga catagcgtca gggcttgctc aacgcggata cccggtaccg gacgtagggc
     2281 aaccagaacg cctttaccgt gggcaggtaa tagagggtta ttcatcatgt tgttgacggc
     2341 attacagaac acatcccact tctggaaacc acgcagtttg agcggctggg tcattgacag
     2401 caaggtagtg atatcttccg gcacatagcg actaaacttc tgcccttgca cgctttcgat
     2461 catcgtcaga cagcgggaga gtggcgcatt ccacgggata accatatttg caccgcaggc
     2521 caataataaa cgttcatcgg tggcgcgcag gctggcggta ttttcccgta cgaggatttt
     2581 catcgcacta ccgcgctggc gacgcagagt atgaatgctg cgggccagtg gctcgatttg
     2641 cgcattttgc tgtaaagaaa agaccaccgt cgccgcctga gcggtacggg cttcattgaa
     2701 caggacttcg ttattgttga acagttgcca gtgttccgat agcggcggcg caccttccag
     2761 tacagcaaca ttactcagaa tgcgtttttc gtcgctgcgt ggttggattt ccgcctcttg
     2821 gtgttgaact aatgtccagc aaccattttg ttgctgaacg ctaagttgct gacgggcgct
     2881 gacccctttt tcgttgcacc agaaagcaat atccagcaaa tgttggtcgc cctgaaaacg
     2941 caaactggca agaccaaaaa gtgaccgata ttcctcaagt aacaatgaaa attgtttatc
     3001 gttattattt ccggaattaa ttaccaaaag cgaacaatgg tttagccggc tccatttatt
     3061 cattttatcc aaccatgagc gaagccgctc ggcaggaatg ttttgccatg cattatttgc
     3121 gcaaacaaga ataaaaaggt aattatgggg atcaatagaa cactgcaaat cacgggccaa
     3181 atagtataga cctttttcat gatttagcat cgaaaataat gttatttttt ccggcccttg
     3241 agaatcatct aattgaaaga ttttcgctgg atcgctgtcc atgctaatga ccgcgacgtg
     3301 tgcggtttca gcctgggatg caattgtttg attcgccaga ctgatagcat cttcatggcg
     3361 atcgacgtta aaccaccaga cgccgcctgc tggcatatgt cgcagctcat cccataatga
     3421 tgagataccg atagagaata cagggtccac aatgtccctc atgtcaacat tttgtctgat
     3481 tatcagttta ctagcgaaag ctaagaaata aacctaccat tgaaattaaa acttatcaga
     3541 tttagcatgt gaatcaattt agcaggtcac aagacatgtg attttagtct tttcgttttt
     3601 aactttttct gttgcataac ggaaagtcaa aaagtgagca cattcccgtc tcgccgtaaa
     3661 caataaaccg acgaaatgct cacagttaga attgtctcat gaacggtacg gttatttcat
     3721 agggatcaag aaaaatgaat aacaatgaac cagatactct gcctgatccc gcgataggct
     3781 atatcttcca gaatgatatt ttggcgttaa agcaggcatt ttcactgcct gatattgatt
     3841 atgccgatat ttcccaacgc gaacagttgg ccgcggcatt aaaacgctgg ccgttgctgg
     3901 cagagtttgc gcaacaaaag taggggattg gtgaatggcc gtactgggat tgcagggggt
     3961 tcggggaggc gtggggacaa caaccatcac cgccgcatta gcctggtcat tacaaatgtt
     4021 gggagaaaat gtcctggtgg tcgatgccag cccggacaac ttgttgcgcc tgtcatttaa
     4081 cgttgatttt acccaccgtc agggttgggc cagagctatg ctggatggcc aggactggcg
     4141 tgacgccggg ttgcgctata cctcgcagct cgatttgctg ccttttggtc agttatcgat
     4201 tgaagaacaa gaaaatccac agcactggca aacccggctg agcgatattt gctccggctt
     4261 acagcaacta aaagccagcg ggcgttacca gtggatttta attgacttac cgcgtgatgc
     4321 ctcgcagata acccaccagc tgctgagttt gtgcgatcac tcgctggcaa tcgtcaatgt
     4381 ggatgccaac tgccatatcc gactgcatca gcaagcgctg ccggatggtg cacatatttt
     4441 gattaatgac ttccgtattg gcagccaggt tcaggacgat atttaccagc tttggttgca
     4501 aagccagcgc cgattgctgc cgatgctcat tcatcgtgat gaagcgatgg ctgaatgtct
     4561 ggcagctaag caaccagtcg gtgaatatcg cagtgatgcg ctggcggctg aagagatact
     4621 gacgctggcg aactggtgcc tgttgaatta ttccgggctg aaaacgccag tcgggagtgc
     4681 atcatgagta tcctgacccg gtggttgctt atcccgccgg tcaacgcacg gcttattggg
     4741 cgttatcgcg attatcgtcg tcacggcgcg tcggctttca gcgcgacgct cggctgtttc
     4801 tggatgatcc tggcctggat ttttattccg ctggagcacc cgcgctggca gcgtattcgc
     4861 gcagaacata aaaacctgta tccgcatatc aacgcctcgc gtccgcgtcc gctggacccg
     4921 gtccgttatc ttattcaaac atgctggtta ctgatcggtg catcgcgcaa agaaacgccg
     4981 aaaccgcgca ggcgggcatt ttcaggtcta caaaatattc gtggacgtta ccatcaatgg
     5041 atgaatgagc tgcctgagcg cgttagccat aaaacacagc atcttgatga gaaaaaagag
     5101 ctcggtcatt tgagtgccgg gacgcggcgg ttgatcctcg gtatcatcgt caccttctcg
     5161 ctgattctgg cgttaatctg cgttactcag ccgtttaacc cgctagcgca gtttatcttc
     5221 ctgatgctgc tgtggggggt agcgctgatc gtacggcgga tgccggggcg cttctcggcg
     5281 ctgatgctga ttgtgctgtc gctgaccgtt tcttgccgtt atatctggtg gcgttacacc
     5341 tcgacgttaa actgggacga tccggtcagc ctggtgtgcg ggcttattct gctcttcgct
     5401 gaaacgtacg cgtggattgt gctggtgctc ggctatttcc aggtcgtatg gccgctgaat
     5461 cgtcagccgg tgccattgcc gaaagatatg tcgctgtggc cgtcggtgga tatctttgtc
     5521 ccgacttaca acgaagatct caacgtggtg aaaaatacca tttacgcttc gctgggtatc
     5581 gactggccga aagacaagct gaacatctgg atcctcgatg acggcggcag ggaagagttt
     5641 cgccagtttg cgcaaaacgt gggggtgaaa tatatcgccc gtaccactca tgaacatgcg
     5701 aaagcgggca acatcaacaa tgcgctgaaa tatgccaaag gcgagttcgt gtcgatattc
     5761 gactgcgacc acgtaccaac gcgatcgttc ctgcaaatga ccatgggctg gttcctgaaa
     5821 gagaaacagc tggcgatgat gcagacgccg caccacttct tctcgccgga tccgtttgaa
     5881 cgcaacctgg ggcgttttcg taaaacgccg aacgaaggca cgctgtttta tggtctggtg
     5941 caggacggca atgatatgtg ggatgccact ttcttctgcg gttcatgtgc ggtgattcgc
     6001 cgtaagccgc tggatgagat tggcggtatt gctgtcgaaa cggtaaccga agatgcgcac
     6061 acttctctgc gtctgcaccg tcgtggctac acctcagcgt atatgcgtat tccgcaggca
     6121 gcggggctgg cgaccgaaag tctctcggcg catatcggtc agcgtattcg ctgggcgcgc
     6181 gggatggtgc aaatcttccg tctcgataac ccgctcaccg gtaaagggct gaagtttgct
     6241 cagcggctgt gctatgtcaa cgccatgttc cacttcttgt cgggcattcc acgactgatc
     6301 ttcctgactg cgccgctggc gttcctgctg tttcatgcct acatcatcta cgcgccagcg
     6361 ttgatgatcg ccctgttcgt gctaccgcat atgatccatg ccagcctgac taactcgaag
     6421 atccagggca aatatcgcca ctctttctgg agtgaaatct acgaaacggt gctggcgtgg
     6481 tatatcgcac caccgacgct ggtggcgctg attaacccgc acaaaggcaa atttaacgtc
     6541 accgccaaag gtggactggt ggaagaagag tacgtcgact gggtgatctc gcggccctac
     6601 atcttccttg ttctgctcaa cctggtgggc gtagcggttg ggatctggcg ctacatctat
     6661 ggtccgccaa ccgagatgct caccgtggtc gtcagtatgg tgtgggtatt ctacaacctg
     6721 attattctcg gcggcgcagt cgcagtatcg gtagaaagca aacaggtacg ccgatcgcac
     6781 cgcgtggaga tgacgatgcc cgcagcaatt gcccgcgaag atggtcacct cttctcgtgt
     6841 accgttcagg atttctccga cggtggtttg gggatcaaga tcaacggtca ggcgcagatt
     6901 ctggaagggc agaaagtgaa tctgttgctt aaacgcggtc agcaggaata cgtcttcccg
     6961 gctcaggtgg cgcgcgtgat gggtaatgaa gttgggctga aattaatgcc gctcactacc
     7021 cagcaacata tcgattttgt gcagtgtacg tttgcccgtg cggatacatg ggcgctctgg
     7081 caggacagct acccggaaga taagccgctg gaaagtctgc tggatattct gaagctcggc
     7141 ttccgtggct accgccatct ggcggaattt gcgccttctt cggtgaaggg catattccgt
     7201 gtgctgactt ctctggtttc ctgggttgta tcgtttattc cgcgccgccc ggagcggagc
     7261 gaaacggcac aaccatcgga tcaggctttg gctcaacaat gatgataacg cgatgaaaag
     7321 aaaaatattc tggatttgtg cagtggctct ggggatgagt gcgttcccct ctttcatgac
     7381 gcaggcgacg ccagcaacgc aaccactgat caatgctgag ccagctgtag ccgcccaggc
     7441 ggagcaaaat ccgcaggtgg ggcaagtgat gccgggcgtg cagggcgcag atgcgccagt
     7501 cgtggcgcag aacggtcctt cccgtgatgt gaagctgacc tttgcgcaaa ttgcgccgcc
     7561 gccgggcagc atggtgctac gtggcattaa cccgaacggc agcattgagt ttggtatgcg
     7621 cagcgatgaa gtggtgacga aggcgatgct caacctcgaa tacacgccat cgccatcatt
     7681 actgcctgtc cagtcgcagt taaaggttta tctcaatgat gaactgatgg gcgtgctgcc
     7741 agtgaccaaa gaacagttgg gtaaaaaaac gctggcacaa atgcccatta acccgctgtt
     7801 tattactgat ttcaaccgtg tgcggctgga gtttgtcggc cattatcagg acgtgtgcga
     7861 aaacccggcc agcaccacgc tttggctgga tgttgggcgg agcagtggac tggatctgac
     7921 ctatcagacc ctgaatgtga agaatgacct gtcacacttc ccggtgccat tctttgaccc
     7981 gcgtgataac cgcaccaaca ccttgccgat ggtctttgcg ggtgcgccgg atgttgagct
     8041 gcaacaagca tctgccattg tcgcctcgtg gtttggttcg cgttctggct ggcgtgggca
     8101 gaacttcccg gtgctctata accaactgcc ggatcgcaat gccattgtct ttgccaccaa
     8161 tgacaaacgc ccggacttcc tgcgcgatca tccggcggta aaagccccgg tgattgagat
     8221 gattaaccat ccgcagaatc cttacgtcaa actgctggtg gtgtttggtc gtgacgacaa
     8281 agacctgttg caggcagcga aaggtatcgc tcagggtaac attctgttcc gtggtgaaag
     8341 tgtggtagtg aatgaagtga aaccgctgct accgcgtaaa ccgtacgatg cgccgaactg
     8401 ggtgcgtacc gatcgtcctg tcacctttgg tgaactgaaa acctatgaag aacaattaca
     8461 atccagcggt cttgagccag cagcgattaa cgtttcgcta aacctgccgc cggatctcta
     8521 cctgatgcgc agtaccggca ttgatatgga tattaattac cgctacacca tgccgccggt
     8581 gaaagacagt tcgcggatgg atatcagcct gaataaccag ttcctgcaat ccttcaacct
     8641 gagcagcaaa caggaggcga accgcctgct gctgcgtatt ccggtattac aaggtttgct
     8701 ggatggcaaa acggatgtct ctattccggc gctgaaactg ggcgcgacca accagttgcg
     8761 gttcgacttt gagtatatga acccgatgcc gggcggttcg gtggataact gtattacctt
     8821 ccagccggtg cagaatcatg tggtgattgg tgacgactcc accatcgact tctcgaagta
     8881 ttaccacttc atcccgatgc cggatctacg cgcctttgct aacgcgggct tcccgttcag
     8941 ccggatggcg gatttgtcgc aaaccatcac cgtgatgccg aaaacgccaa acgaagcaca
     9001 gatggaaacc ttgctgaata ccgttggctt tatcggtgcg cagacgggct tcccggcgat
     9061 taacctgacg gtgaccgatg atggcagcac cattcagggc aaagatgccg acatcatgat
     9121 cgtcggtggt atcccggaca aactgaaaga cgataagcag atcgacctgt tggtgcaggc
     9181 gaccgaaagc tgggtgaaaa cgccgatgcg ccagaccccg ttccccggta ttgtgccgga
     9241 cgagagcgat cgcgcggcag aaacccagtc aacgctgacc tcttccggtg cgatggcggc
     9301 ggtgattggc ttccagtcgc cgtataacga ccagcgcagc gtgattgcgc tgctggcaga
     9361 tagcccacgc ggttatgaaa tgcttaacga tgcggtgaac gatagcggca aacgcgccac
     9421 catgttcggt tcggtcgcgg tgatccgcga gtccggtatc aacagcctac gtgttggcga
     9481 tgtttattac gtaggtcatc tgccgtggtt cgagcgcttg tggtatgcgc tggcaaacca
     9541 tccgattctg ctggcggtgc tggcggctat cagtgtgata ttgctggcgt gggtactgtg
     9601 gcgtctgctg cgtattatta gtcgtcgtcg tcttaacccg gataacgagt aattgaagat
     9661 gaatgtgttg cgtagtggac tcgtgacgat gctgctgctg gctgccttta gtgttcaggc
     9721 agcctgtacc tggcctgcct gggagcagtt taaaaaggat tacatcagtc aggaagggcg
     9781 cgtcatcgac cccagcgacg cgcgcaaaat caccacctcc gaagggcaaa gttacggcat
     9841 gttctttgcc ctggcggcta acgaccgtgc agctttcgat aatcttctcg actggacgca
     9901 gaacaatctc gctcagggtt ctttaaaaga aaatttgccc gcctggctgt ggggcaagaa
     9961 agagaacagt aagtgggaag tgctggacag caattcggcc tccgatggtg atgtctggat
    10021 ggcctggtcg ttgctggagg cggggcgttt gtggaaagag cagcgttata ccgacatcgg
    10081 cagcgcattg ctaaaacgta tcgcgcggga ggaagtggtg acggtgcctg ggctgggctc
    10141 catgttgtta ccgggcaaag tgggttttgc tgaggataac agctggcgtt ttaaccccag
    10201 ctacctgccg ccgacgctgg cgcagtattt cacccgcttt ggcgcgccgt ggaccacgct
    10261 gcgcgaaacc aatcaacgtt tattgctgga aaccgccccg aaaggctttt cgccagactg
    10321 ggtgcgctat gagaaagaca aaggctggca gctaaaagcc gaaaaaacgt tgatcagcag
    10381 ctacgacgct atccgcgttt acatgtgggt aggcatgatg cctgacagcg atccgcaaaa
    10441 agcgcggatg ctcaaccggt ttaaaccgat ggcgacattc actgagaaaa acggttatcc
    10501 gccggaaaaa gtggatgtgg ctacggggaa agcgcagggt aaaggaccgg tcggtttttc
    10561 tgccgccatg ctgccctttt tacaaaaccg cgatgcgcag gccgttcagc gccagcgcgt
    10621 ggccgataac tttcccggca gcgatgccta ttacaactat gtgctgaccc tgtttggaca
    10681 aggctgggat caacaccgtt tccgcttctc gacaaaaggt gagttattac ctgactgggg
    10741 ccaggaatgc gcaaattcac actaaacata ttcacgcttt ccctcggtct ggccgtcatg
    10801 ccgatggtcg aggcagcacc aaccgctcag caacagttgc tggagcaagt tcggttaggc
    10861 gaagcgaccc atcgtgaaga cctggtgcaa cagtcgttgt atcggctgga acttattgat
    10921 ccgaataacc cggatgtgat tgctgcccgt tttcgttctc tgttacgtca gggcgatatt
    10981 gatggcgcac aaaaacagct cgatcggctg tcgcagttag cgccgagttc aaatgcgtat
    11041 aaatcgtcgc ggactacgat gctgctttcc acgccggatg gtcgtcaggc actgcaacag
    11101 gcaagattgc aggcaactac cggtcatgcg gaagaagctg tggcgagtta caacaaactg
    11161 ttcaacggtg cgccgccgga aggtgacatt gctgtcgagt actggagtac ggtggcgaaa
    11221 attccggctc gccgtggcga agcgattaat cagctaaagc gcattaatgc ggatacgccg
    11281 ggcaacacgg gcctgcaaaa caatctggcg ctattgctgt ttagtagcga tcgccgtgac
    11341 gaaggctttg ccgtcctgga acaaatggcg aaatcgaacg ccgggcgcga aggggcctct
    11401 aaaatctggt acgggcagat taaagacatg cccgtcagcg atgccagcgt gcaggcactg
    11461 aaaaaatatc tctcgatctt tagcgatggc gatagcgtgg ctgctgcgca atcgcaactg
    11521 gcagaacagc aaaaacagct ggccgatcct gctttccgcg ctcgtgcgca aggtttagcg
    11581 gcggtggact ctggcatggc gggtaaagcc attcccgaac tacaacaggc ggtgcgggcg
    11641 aacccgaaag acagtgaggc tctgggggcg ctgggccagg cgtattccca gaaaggtgat
    11701 cgcgccaatg cagtggcgaa tctggaaaaa gccctcgcgc tggatccgca caacagcaat
    11761 aacgacaaat ggaacagtct gctgaaagta aaccgctact ggctggcgat ccagcagggc
    11821 gatgccgcgc tgaaagccaa taatcctgac cgggcagaac ggctgttcca gcaggcacgt
    11881 aatgtcgata acaccgacag ctatgcagtg cttgggctgg gcgatgtggc gatggcgcgc
    11941 aaagattatc ccgccgccga acgctattac cagcagacct tgcgtatgga cagcggcaac
    12001 actaacgccg tgcgcgggct ggcgaatatt taccgccagc agtcgccaga aaaagccgaa
    12061 gcgtatatcg catcgctctc tgccagccag cggcgcagca ttgatgatat cgaacgcagc
    12121 ctgcaaaacg accgtctggc acagcaggca gaggcactgg aaaaccaggg gaaatgggcg
    12181 caggcggcag cacttcagcg gcaacgactg gcgctggacc ccggcagtgt atggattact
    12241 taccgacttt cgcaggatct ctggcaagcc ggacaacgca gccaggccga tacgttaatg
    12301 cgcaatctgg cgcagcagaa gccgaacgac ccggagcagg tttacgctta cgggctgtat
    12361 ctctccggtc acgaccagga cagagcggcg ctggcgcata tcaacagcct gccgcgcgcg
    12421 cagtggaaca gcaatattca ggagctggtt aatcgactgc aaaacgatca ggtgctggaa
    12481 accgctaatc gcctgcgaga aaacggtaaa gaggcagaag cggaagcgct gctgcgccag
    12541 caaccgcctt cctcgcgtat tgacctcacg ctggctgact gggcgcagca gcgacgtgat
    12601 tacaccgccg cccgcgcggc ttatcagaac gttttgacgc gcgagcccac taacgccgat
    12661 gccattcttg gtctgacgga agtggatatt gctgccggtg acaaagcggc ggcacgtagc
    12721 cagctggcga aactgcctgc cactgataac gcctcgctga acacacagcg gcgcgtggcg
    12781 ctggcacagg cgcagcttgg cgataccgca gcggcgcagc agacatttaa taagttgatc
    12841 ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga tggtactgcg tgatggtgcg
    12901 aagtttgaag cgcaggcggg cgatccaatg caggcgctgg aaacctacaa agacgccatg
    12961 gttgcatccg gtgtgaccac gacgcgtccg caggataacg acacctttac ccggctgacc
    13021 cgtaacgacg agaaagatga ctggctgaaa cgcggcgtgc gcagcgatgc ggcggacctc
    13081 tatcgccagc aggatcttaa cgtcactctc gagcacgatt actggggttc gagcggcacc
    13141 ggtggttact ccgatctgaa agcgcacacc actatgttgc aggtggacgc gccctattct
    13201 gacgggcgga tgttctttcg cagtgatttc gtcaatatga acgtcggcag tttctccact
    13261 aatgccgatg gcaaatggga tgacaactgg ggcacctgta cattacagga ctgtagcggc
    13321 aaccgcagcc agtcggattc cggtgccagc gtggcggtcg gctggcgaaa tgacgtctgg
    13381 agctgggata tcggtaccac gccgatgggc ttcaacgtgg tggatgtggt cggcggcatc
    13441 agttacagcg atgatatcgg gccgctgggt tacaccgtta acgcccaccg tcggcctatc
    13501 tccagttctt tgctggcctt tggtgggcaa aaagactctc cgagcaatac cgggaaaaaa
    13561 tggggcggcg tgcgtgccga cggcgtgggg ctaagtctga gctacgataa aggtgaagca
    13621 aacggcgtct gggcatcgct tagcggtgat cagttaaccg gtaaaaatgt cgaagataac
    13681 tggcgcgtgc gctggatgac gggctattac tataaggtca tcaaccagaa caatcgccgc
    13741 gtcacaatcg gcctgaacaa catgatctgg cattacgaca aagacctgag tggctactca
    13801 ctcggtcagg gcggttacta cagtccgcag gaatacctgt cgtttgccat accggtgatg
    13861 tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg cgtctggctc gtggtcgcat
    13921 tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc tgatcccgac cgactggcag
    13981 gaagaagctg cgcggcaatc caacgatggc ggcagcagtc agggctttgg ctacacggcg
    14041 cgggcattac ttgaacgacg tgttacttcc aactggtttg tcggcacggc aattgatatc
    14101 cagcaggcaa aagattacgc acccagccat ttcctgctct acgtacgtta ttccgccgcc
    14161 ggatggcagg gtgacatgga tttaccaccg cagccgctga taccttacgc cgactggtaa
    14221 gttttcagat agcgcctctc ttaatgccgc tgcgatcggg tatactcggg cggcaatctg
    14281 ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt taacaatcaa gcagatggca
    14341 atggtggcag ccgttgtcct ggtgttcgtt tttatttttt gcaccgtttt gctgttccat
    14401 ctggtccagc agaatcgcta taacacggct acgcaactgg aaagcattgc tcgctctgtc
    14461 cgcgaaccct tatcttctgc cattttgaaa ggcgatattc ccgaagcgga agctattctt
    14521 gccagcatta aaccggcagg cgtggtcagc cgtgccgatg tagtgctgcc caaccagttc
    14581 caggcgctgc gtaaaagttt tattccagag cgtccggtgc cggtaatggt tactcgcctg
    14641 tttgagctac cggtgcaaat ctcgctgggc gtttactcgc tggaacgtcc ggcaaacccg
    14701 cagccaattg cctatctggt gctacaggcg gattccttcc gcatgtataa gttcgtgatg
    14761 agcaccctct caacgttagt gaccatttac ttacttttgt cgttaatatt gactgtggcg
    14821 attagctggt gcattaaccg cctgattttg catccattac gcaatattgc tcgcgaactt
    14881 aacgccatcc caccccagga tcttgttggt caccaactgg cattaccgcg tctgcatcag
    14941 gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca accagcaatt gctgcagcgc
    15001 cattatgaag aacagaacga aaatgcgatg cgcttcccgg tgtcggattt gccgaacaaa
    15061 gccttgctga tggagatgct ggagcaggtg gtggcgcgta aacaaaccac cgcgctgatg
    15121 atcatcacct gtgaaaccct gcgtgatact gcgggtgtgc tgaaagaggc gcaacgagaa
    15181 attctgctgc tgacgctggt ggaaaaactc aaatcggtac tgtcgccacg catgatcctc
    15241 gcgcagatta gcggttatga ctttgctgtc attgccaacg gtgtacagga accgtggcac
    15301 gctatcacat taggtcagca agtgctcact atcatgagcg agcgcctgcc gattgaacgt
    15361 attcaactcc gtccgcactg tagcattggc gtggcgatgt tctacggcga tctcaccgcc
    15421 gaacagcttt acagtcgcgc tatttctgcg gcatttaccg ctcgccataa aggcaaaaat
    15481 cagattcagt tctttgatcc gcagcagatg gaagccgccc agaagcggtt gacggaagag
    15541 agcgatatcc ttaatgcact ggaaaatcat cagtttgcga tttggttaca gccacaggtc
    15601 gagatgacca gcggtaaact ggtcagtgcg gaagtgttac tgcgtatcca gcaaccggat
    15661 ggcagttggg acctgccgga tggcttaatc gatcgcattg agtgctgtgg gctgatggtt
    15721 accgtcggtc actgggtgct ggaagagtcc tgtcgattgc ttgctgcctg gcaagagcgc
    15781 ggcattatgc tgcccttgtc ggtaaacctc tcagcgctgc aactgatgca tccaaatatg
    15841 gtggcggata tgctggaact gttaacccgc tatcgcattc agccgggaac actgattctg
    15901 gaagtgacag aaagccgacg tattgacgat cctcatgctg cggtggcaat cctccgtccg
    15961 ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg gcatgggcta cgcagggctg
    16021 cgtcagctgc agcatatgaa atcgttgcca atcgacgtac tgaaaatcga caaaatgttt
    16081 gttgaaggtt tgccggaaga tagcagcatg attgctgcaa ttatcatgct ggcgcagagc
    16141 ctgaacctac aaatgattgc cgaaggcgtg gagactgaag cacaacgcga ctggctggca
    16201 aaagcgggcg ttggtattgc ccagggcttc ctttttgctc gcccactccc tattgaaatc
    16261 ttcgaagaga gttacctgga agaaaaatag ctaccccaaa ctgattacaa aactttaaaa
    16321 agtgctggtt tgtgcgagcc agctcaaact ttttaacctt tttgtttcaa ttatgatcca
    16381 ggtacatttc tgtgatgttg tctgggtgtt attttaaggc cgcaggtacc ccataacctt
    16441 acaagacctg tggttttact aaaggacacc ctatgaaaac ctctctgttt aaaagccttt
    16501 actttcaggt cctgacagcg atagccattg gtattctcct tggccatttc tatcctgaaa
    16561 taggcgagca aatgaaaccg cttggcgacg gcttcgttaa gctcattaag atgatcatcg
    16621 ctcctgtcat cttctgtacc gtcgtaacgg gcattgcggg catggaaagc atgaaggcgg
    16681 tcggtcgtac cggcgcagtc gcactgcttt actttgaaat tgtcagtacc atcgcgctga
    16741 ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc cggaatgaac gtcgatccgg
    16801 caacgcttga tgcgaaagcg gtagcggttt acgccgatca ggcgaaagac cagggcattg
    16861 tcgccttcat tatggatgtc atcccggcga gcgtcattgg cgcatttgct agcggtaaca
    16921 ttctgcaggt actgctgttt gccgtactgt ttggttttgc gctccaccgt ctgggcagca
    16981 aaggccaact gatttttaac gtgatcgaaa gtttctcgca ggtcattttc ggcatcatca
    17041 atatgatcat gcgtctggca cctattggtg cgttcggggc aatggcgttt accatcggta
    17101 aatacggcgt cggcacactg gtgcaactgg gacagctgat tatctgtttc tacatcacct
    17161 gtatcctgtt tgtggtgctg gtattgggtt caatcgctaa agcgactggt ttcagtatct
    17221 tcaaatttat ccgttacatc cgtgaagaac tgctgattgt actggggact tcatcttccg
    17281 agtcggcgct gccgcgtatg ctcgacaaga tggagaaact cggctgccgc aaatcggtgg
    17341 tggggctggt catcccgaca ggctactcgt ttaatcttga tggcacatcg atatacctga
    17401 caatggcggc ggtgtttatc gcccaggcca ctaacagtca gatggatatc gtccaccaaa
    17461 tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc ggcaggggta acgggtagtg
    17521 gttttatcgt gctggcagcg acgctctctg cggtgggcca tttgccggta gcgggtctgg
    17581 cgctgatcct cggtatcgac cgctttatgt cagaagctcg tgcgctgact aacctggtcg
    17641 gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa agaactggac cacaaaaaac
    17701 tggacgatgt gctgaataat cgtgcgccgg atggcaaaac gcacgaatta tcctcttaat
    17761 ctcaccactt atgcccgcac tcccttcccc gagtgcgggt gtttgcgcat aattgacccc
    17821 tgtgcctgaa cttaccacct gggtgagttc aggtttattt cacttcttcc gtgacatttt
    17881 catgttcttg cggtctaaca cgaagtgttt ttaacgtcat ttagactgac cgacaacggg
    17941 cagtttttta ttaccaggat agttgatcag gggttcacat gcagggcaca aaaattcgac
    18001 ttttagcggg cggtttgctg atgatggcca ctgctggcta tgtgcaggca gatgcgctcc
    18061 agcctgatcc agcatggcaa caggggacgc tttccaacgg tttacagtgg caagtgctga
    18121 ctacacccca gcgtcccagc gatcgtgttg aaattcgcct gctggttaat accggttcgc
    18181 tcgccgaaag tacacaacag agcggttaca gtcacgccat ccctcgtatt gcgctaacgc
    18241 aaagcggtgg ccttgacgca gcgcaggcgc gttcattgtg gcagcagggg atcgacccta
    18301 aacgcccgat gccgccggta attgtctctt atgacaccac gctgtttaat ctgagtttgc
    18361 ccaataaccg taacgacttg ctgaaagagg cgctctctta tctggcaaat gccactggca
    18421 aactgactat cacgccagaa accatcaacc atgcgttgca aagtcaggac atggtggcaa
    18481 cctggcctgc cgatactaaa gagggctggt ggcgttatcg tctgaaagga tcaaccttgt
    18541 taggtcacga tcctgccgat ccgctgaaac aacccgttga agcggaaaag attaaagatt
    18601 tctatcagaa atggtacacc ccggatgcaa tgacgctgct ggtggtggga aacgtggatg
    18661 cgcgctcggt cgtcgaccaa atcaataaaa cgtttggcga actgaaaggc aaacgtgaaa
    18721 caccggctcc ggtgccgacg ctttctccgc tgcgtgcgga agcggtgagt attatgaccg
    18781 acgcggtgcg tcaggaccgg ttatctatca tgtgggatac gccgtggcag ccgattcgtg
    18841 aatcagccgc actgctgcgc tactggcgtg cggacctggc ccgtgaggcg ctgttctggc
    18901 atgttcagca agcgttaagc gccagtaaca gtaaagacat cggtcttgga tttgactgtc
    18961 gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga atcaccaaac gacaagctga
    19021 acagcaacct taatctggtg gcgcgtgaac tggcgaaggt tcgcgataaa ggtctgccgg
    19081 aagaagagtt caatgcgtta gtggcgcaaa agaaactgga gctgcagaag ctgtttgccg
    19141 cctatgcgcg agctgatacc gatattctga tggggcagcg gatgcgttcg ttgcaaaatc
    19201 aggttgtcga tatcgcgccg gagcaatatc agaaactgcg tcaggatttc cttaacagcc
    19261 tgacggtgga gatgttaaat caggatctgc gtcagcagtt gtcgaatgat atggcgttaa
    19321 tactgctgca gccgaaaggc gagccggaat ttaacatgaa agcgttgcag gcggcctggg
    19381 atcaaatcat ggccccatcg actgcggctg ccgccacatc tgtcgccacg gatgacgtac
    19441 atcctgaagt gacggatatt ccacctgcac agtaatgcct gatggtgatg agttagcaca
    19501 accatcaggt atttatggat gagctggata ggggggtaac actctatcca gccttttgca
    19561 tatgctgctt ttacgctggc atcgcttcac gcgggataat cgcgccgcga tactgaataa
    19621 cggtacttgc ggtcaggtgc ccgcgtttcg ctgcgtcttc cgcactgccg cccgtcaaac
    19681 gtaccgccag ataaccggca ctgaaagagt cgccagctgc ggtggtatcg atcacttttt
    19741 ctttcggcag ttttaccgcc ggaacatcca ctaacccttc accagcaatg gacaccaggc
    19801 aagaatccgc cccgcgtttc accaccactt ctttcacgcc cgcgttatgg gtgcgcgcaa
    19861 tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc atcgtccagc gtcaggaagg
    19921 cgatatccgt gcattccagc atttgttggt acacctgctg tgtctcttct ttgctggccc
    19981 acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc gccgttggcg cggcattcgc
    20041 gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa gatcgccagg ctaatcccgc
    20101 tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat cgtcgcggat tgctcactct
    20161 ccagccagaa tttggcggcg gcttcgttac gccagtagta gaacgtacgt tcgccggtgc
    20221 tgtcggtttc aatgtagtaa aggcccggca gacggttttc catccgttgg gtcagggaag
    20281 tatcaacgtt ctcgccgcgc caggcgtcca gcatctgctg gctaaaactg tccgttccca
    20341 gcgccgttac gtaatgaacg gttaatgccg caggatcgac ctgacgggcg atatagacgg
    20401 aagtgttcag ggtatcgccc ccgaaaccgc gcttaacgtc cgcgcctttc tcggaaagct
    20461 caatcatgca ttcgccaatc acggcaatct ttttggacat agttgtgaac ctgatctgta
    20521 aaaaattagc gttagtttgc gctgtggtgg tttgctggtc aaccatatta aaacagtgtt
    20581 ccattatttt tttgagccag aacgtattcc tgaaagattc cgttgtggag tggggaaatt
    20641 taccccggcc aggcggtaaa gttctgcctt tgcgcgccga taatctttgt cgagtccggg
    20701 cagcatcact tttaaacaca ggacatcttt gatgataagg caggttatcc agcgaataag
    20761 caaccctgaa gcaagcatcg agagcttgca ggaacggcgt ttttggttgc agtgtgagcg
    20821 tgcttacacc tggcagccga tctatcaaac atgcgggcgg ttaatggccg tggagctatt
    20881 aacggtggtc acgcatccct tgaacccttc gcaacgcctg ccgccggatc gctattttac
    20941 tgaaatcacc gttagccatc ggatggaggt tgtgaaagag cagattgatt tgctggcgca
    21001 aaaagccgac ttctttgtag agcacggcct gctggcatcg gtcaatattg atggccctac
    21061 gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccag attgaggagc ttccctggct
    21121 gcgtttcgaa ctggtggaac acatccgcct gccgaaagat tcaacctttg cctcgatgtg
    21181 tgaatttggc ccgctgtggc tggatgattt tggtaccggg atggcaaatt tctctgcact
    21241 aagtgaagtg cgttatgact acatcaaaat cgcgcgagaa ctgtttgtga tgctgcgtca
    21301 gtcaccggaa ggacgcacac tcttttctca gcttttacat ctaatgaatc gctattgtcg
    21361 cggggtgatt gtcgagggcg tagaaacacc agaagagtgg cgtgatgttc agaactcgcc
    21421 cgcattcgcc gcacaaggct ggtttctttc acgcccggca ccgatagaaa cgctgaatac
    21481 ggcggttctg gcgctataag ctgcctcatt ttccgcctgg ctggactatc tttaggactg
    21541 gcacagaaag aatatgagga agcgaacgat gagcaaggca ggcaaaataa ccgctgcgat
    21601 ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt ttgattgcaa cttttgactg
    21661 gaatcgactc aaaccgacca tcaaccagaa agtctctgcg gagttgaatc gtccgttcgc
    21721 tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa caagaaactg gctggcgcag
    21781 ctgggtgccg tggccccatg tacacgcgga agacatcatt cttggcaatc caccggatat
    21841 tcccgaagtc acaatggtgc atttgccgcg agtagaagcg acgctggccc cgctggcgct
    21901 gctgaccaaa acggtctggt tgccgtggat caagctcgaa aagcccgacg cgcgcctgat
    21961 tcgcctctcc gaaaagaaca ataactggac gtttaatctc gccaacgatg acaacaaaga
    22021 cgcgaatgca aagccgtcgg cgtggtcgtt tcggctggat aatattctgt tcgatcaagg
    22081 gcggatcgcc attgatgaca aagtaagcaa agcggatctg gaaatttttg tcgatccgtt
    22141 aggcaagccg ctgtcgttca gcgaagttac cggatcgaaa ggtaaagcgg ataaagaaaa
    22201 ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt tataacggtg aaccgctcac
    22261 tggtacggga aaaataggcg gtatgctggc gctgcgtggc gaaggaacgc cgtttccggt
    22321 acaggctgat ttccgctctg gtaacacccg tgttgctttt gatggcgttg tgaatgaccc
    22381 aatgaagatg gggggtgtcg atttacggct taaattttct ggcgattcgc tgggtgatct
    22441 ctatgaactg acgggcgttc tgctgcccga tacaccgccg tttgaaacgg atggtcggct
    22501 ggtagcgaaa atcgatactg aaaaatcgtc ggtctttgat tatcgcggtt ttaatgggcg
    22561 cattggcgat agcgatatcc acggttctct gatctacacc accggcaagc cacgaccaaa
    22621 actggaaggt gatgtcgagt cgcggcaatt gcggctggcg gacctgggac cgttgattgg
    22681 cgttgattcc gggaaagggg cagaaaagtc gaaacggtct gaacagaaga agggcgaaaa
    22741 aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc ttcgaaaccg ataaatggga
    22801 cgttatggat gccgatgttc gcttcaaagg gcggcgcatt gagcatggca gcagcctgcc
    22861 gattagcgat ctttctactc atatcatcct caaaaatgct gacctgcgcc tgcaaccgct
    22921 gaaatttggc atagcgggcg gcagcattgc ggcgaatatt catctggaag gcgataaaaa
    22981 gccgatgcag gggcgggcag atattcaggc tcgtcgactg aaactgaaag aactgatgcc
    23041 cgatgtagaa ctgatgcaga agacgctggg ggaaatgaac ggtgacgcgg aactgcgcgg
    23101 tagcggtaac tcggtggcgg cgcttttagg caacagtaac ggcaacctga aactgttgat
    23161 gaatgacggg ctggtgagcc gcaacctgat ggagattgtt gggctgaatg tcggcaacta
    23221 cattgtcggt gcgatatttg gtgatgatga ggtgcgggtg aactgcgcgg cggcgaatct
    23281 ggatattgcc aacggcgtgg cgcgcccgca gatttttgct ttcgatactg agaacgcgtt
    23341 gattaacgtt accggtacgg caagttttgc ttcggaacag ctggatttga ctattgatcc
    23401 ggagagtaaa gggattcgga ttatcacatt gcgttcgccg ctgtatgtac gtgggacgtt
    23461 taaaaatccg caggctgggg tgaaagccgg gccgttgatt gctcgcggag ccgttgcggc
    23521 ggcactggca acgctggtaa caccggcggc ggcgttactg gcgctgatct caccttccga
    23581 aggggaggct aatcagtgca ggacaatttt gtcgcagatg aagaagtgat gataattgcc
    23641 ggatgtctga catccggcaa ttaaacagat ttagattgtt ggcaaagtgt gctttgttca
    23701 tgccggatgc ggcgtgaacg cctccggctt acaaatcctt gcgaattcaa tatattgtat
    23761 ggaaattctg gcctgataag cgtagcgcat caggcttttc actcttatta caacgactga
    23821 tgtcgcgtct catgggtcag cagcagggcg attaacgtca agccagccat cgccgccaga
    23881 tatagcccca ccgcacctaa cccgtagtta gtctgcaacc aggctgcgat atacggcgca
    23941 acggaagccc cgagaatcga cgatacgttg taagagaacg atgctccggt gtaacgcact
    24001 tctgtcggga acagctctgg taacagcgcg cccatcggac cgaaggtcag ccccatcaga
    24061 cttaacccca gcagcagaaa ggcaaaaacc agaatcgggt tgccagaacc gagcaaaggg
    24121 gtaaaggcga atagcgcgaa caggatgatc agcgtggtga tgatcaccat gcttttacgg
    24181 cgaccaaagg catcagccag taatccggcg actggcacca tcacgccaaa accaataact
    24241 gccatcatca gcatccacaa cacttcgtta cgcggcaggc caagcccaac tggcgcggcg
    24301 gcggtactaa aggtcataga gtagaccgtc atgatgtaaa acagcgtata ggttgccagc
    24361 ataatgaacg tacccagtac ggttacgcga acatgtttgg tcagcagcgt acccagcggg
    24421 atcttcacct gttttttcgc tttagcgact ttctcaaaca ccggcgactc atgcagcgac
    24481 acgcgaacat acaggccgat aatgaccagc accgccgaga agataaatgg cacacgccag
    24541 ccccagctca taaactgctc gtcggtcagt agccaggaaa gcagcaagaa agtgccgttg
    24601 gcaaagaaga agccaatcgg tgcgcccagc tgcgggaagg agccgtacaa tgcacgtttg
    24661 cgcggtgggg cgttttcagt cgccagcagc gccgcgccgc cccattcacc acctaagccc
    24721 agaccctgac caaatcgagc cagcgccagc agcagcgggg cgaaaatacc aatcgtggca
    24781 tagcctggca gcagaccaat caccacggtc gaaatcccca tcgttagcaa cgaggcgacc
    24841 agcgtcgctt tacgcccaac gcgatcgcca aaatgaccaa aaacggcaga gccaatgggg
    24901 cgcgcgacga aggcgatggc gaaggtggcg agcgactgta gcgttgctgc cgttggatcg
    24961 ccctgcggga agaagatatg cggaaacaca ataacggccg cagtggcgta aatgtaaaag
    25021 tcgaagaact caatggccgt gccaatgaga gaggcgacaa ggactttatt acgcgagttg
    25081 atcggcgtgt attcttgctc gtggtcgagt gttgtggctg ttgcttgcat aatcttttct
    25141 tattttttgg ctaacgaata gccatattac gcacagcaaa agtgatattt caatgtgtaa
    25201 caaatcgcgg aaatggtcaa aaatcaggca aaaagggaat aatgtttagg ccagtgattt
    25261 ttcttctatg aaatgcttca cataatttta taaatggtgg attaaactgg ttttaggtta
    25321 aataaaagtt accaactgga tttatatgct ggggttatga gttggaatga aattgtttgc
    25381 tcctgaatag atggctaaag aggctctggg aaagagtttc aagaggttat gtgcgcatta
    25441 tagccggatg cgatgctggc gcatcttatc cggcctacgg ggagccaggc cggataaatt
    25501 cgtacgccgc atccggccta ttttaaggct gtgttttccc cggcattcgc gggtcgtctt
    25561 tatattcggc ggtggcaatc cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga
    25621 aaaatgccat cagccccagc acggaaccaa atgctgcgcc agagggtgat ttcatcaacg
    25681 atggcagggt gtaggtcatc acgattttaa tcacttcaaa accaatagca gccagaaatg
    25741 ttccgcggat cagggctttt ttgcgcggac ggtggcgcgg caaccgccag aagatccaga
    25801 agaaaagcag atagttggcg aagatggaaa tcgccacgcc aatcaaccgc catgtcggtt
    25861 tcagccactc aatgctgttc aggtgcaggg cgctaataat catttgctgc gcagaaccgg
    25921 caaccgaggt gatagaaagc gtcacaatca gcgcaatcaa caaaccaatc agcgaaataa
    25981 aatcacgcag atattttacc cagaacttct cctgatcttg cggcgagcgt tcccagacat
    26041 cgcgtgactg ggcgcgaatc gcttcacgca ggttacccat ccagttgatg ccggaataaa
    26101 gtgccactgc cagaccgaca agccctacag tcgtacgctg ctgaacggcg gtgttgatgg
    26161 tgtttttcaa tgtggcggct agcgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga
    26221 tatcctgtag caacatcgga tgggaggcga gcacaaagcc ccctgcggca aacgacacca
    26281 tcaaaatggg gatcatcgac aaaaatgaga aataggtgat agccgcacca aactggttac
    26341 ccagccgatc gttaaagcgt tctgtcgcgc gaatcaggtg cgcaataacc ggttggcgtt
    26401 ggactttctc ggcagtggtg gtgacggttt ccagcgcctg gctaactttg ccgcctttct
    26461 cgctattatc caactgctta atcggctcgt gctccagatc ctgggtggga cgtttgatct
    26521 cgttttcctg cgtcatccgc tcttttgtcc ttctgtcgtg gtcattctta aaagtatagt
    26581 cggtcagtcc aggtactctt tcattacgcc agccagccac tgcataaaca ggtttacacg
    26641 ccgggaaagc tcccgacgct gcggataaac cagtgaaagg gacagcggct cggcacggta
    26701 gccaggcaac acttcaataa gccgcccggc acgcagggct tcgcgcacgg caatgcgcgg
    26761 gatctgaata atccccagac cggcaagacc ggcggcgaga tagttttcgc tactgtttac
    26821 cgtcaacatg ctgccagact taaaccactg gacgccatta acgctggcaa cctcaaaacc
    26881 taacggatgt acacccaggt gcggtgtata acgcactatt gcgtgtgaag tcagatcatc
    26941 ggggctttgc ggataaccaa agcgcgtcag atagtgcgga ctggcacagt tgaccatggt
    27001 cagtttaccg aggggacggg cgataacgcc atcttccggt aatgcgccag tgcgtatcac
    27061 gcaatcaaaa ccatcatgaa gaatatctac cggacggtca tggctactca gttccagctc
    27121 aattcccgga tgcagataga gaaattccga caagcgcggc agtaacaggc ttttcgcgat
    27181 tcctgacggg atgtcgatgc gtaatttacc gctgatactg gtggcatcct gttgaaacag
    27241 accgtccagt tcgctgagat tactcaacac atcttttgct cgttgataat aggtcatgcc
    27301 ttctggcgtg agtttgaccc gtcgcgtggt gcgctggaga agctgggtgc caagttgatg
    27361 ttccagtgcc tgtatctggc gcgaaacact tccctttggc aaagcaaaga aatccgccgc
    27421 gcgggaaaaa ctttccagct ccgcgacttt gatgaacaac tgcattgcgt gaattttatc
    27481 catttctttg accgattgtt gttcacaacg aaacagtagc gtgcatatcg cattgtttat
    27541 tgttttttct aacatctaat gccgggtttc acaatgttgt catgatgaat gtggcgattt
    27601 ctcagcagga aaagggagga atagctttat gagaaaggta aat
//
LOCUS       DACADF010000059        27522 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.059, whole genome shotgun sequence.
ACCESSION   DACADF010000059 DACADF010000000
VERSION     DACADF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.059"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(110..388)
                     /locus_tag="ISB30_002309"
     CDS             complement(110..388)
                     /locus_tag="ISB30_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989938.1"
                     /translation="MSTFNTRKDRDRNGKAKPHKHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            complement(747..1037)
                     /locus_tag="ISB30_002310"
     CDS             complement(747..1037)
                     /locus_tag="ISB30_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1989939.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1038..1349)
                     /locus_tag="ISB30_002311"
     CDS             complement(1038..1349)
                     /locus_tag="ISB30_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP1989940.1"
                     /translation="MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1578..2486
                     /locus_tag="ISB30_002312"
     CDS             1578..2486
                     /locus_tag="ISB30_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1989941.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2550..3491)
                     /gene="fabY"
                     /locus_tag="ISB30_002313"
                     /gene_synonym="yiiD"
     CDS             complement(2550..3491)
                     /gene="fabY"
                     /locus_tag="ISB30_002313"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAP1989942.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3536..3973)
                     /gene="dtd"
                     /locus_tag="ISB30_002314"
     CDS             complement(3536..3973)
                     /gene="dtd"
                     /locus_tag="ISB30_002314"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP1989943.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQDMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3970..4842)
                     /locus_tag="ISB30_002315"
     CDS             complement(3970..4842)
                     /locus_tag="ISB30_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAP1989944.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4836..5435)
                     /gene="yihX"
                     /locus_tag="ISB30_002316"
     CDS             complement(4836..5435)
                     /gene="yihX"
                     /locus_tag="ISB30_002316"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAP1989945.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLRAEGFSPDD
                     TVFFDDNADNIEGANRLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5576..6463)
                     /locus_tag="ISB30_002317"
     CDS             complement(5576..6463)
                     /locus_tag="ISB30_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAP1989946.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLTSPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQVGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMTSSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(6453..7802)
                     /locus_tag="ISB30_002318"
     CDS             complement(6453..7802)
                     /locus_tag="ISB30_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAP1989947.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIASFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7901..9334)
                     /locus_tag="ISB30_002319"
     CDS             complement(7901..9334)
                     /locus_tag="ISB30_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1989948.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9404..10414)
                     /locus_tag="ISB30_002320"
     CDS             complement(9404..10414)
                     /locus_tag="ISB30_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP1989949.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQQLKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10447..11334)
                     /locus_tag="ISB30_002321"
     CDS             complement(10447..11334)
                     /locus_tag="ISB30_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP1989950.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(11359..12237)
                     /gene="yihU"
                     /locus_tag="ISB30_002322"
     CDS             complement(11359..12237)
                     /gene="yihU"
                     /locus_tag="ISB30_002322"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAP1989951.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNTQAVDALVTQG
                     ATAAQTPAEAAASAEFIITMLPNGDIVRQVLLGEKGVCETVSSDALVIDMSTIHPLQT
                     DALIRELQEKGINMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARKTGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12410..13306
                     /locus_tag="ISB30_002323"
     CDS             12410..13306
                     /locus_tag="ISB30_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1989952.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            13346..14134
                     /locus_tag="ISB30_002324"
     CDS             13346..14134
                     /locus_tag="ISB30_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1989953.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFTA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(14242..16065)
                     /gene="typA"
                     /locus_tag="ISB30_002325"
     CDS             complement(14242..16065)
                     /gene="typA"
                     /locus_tag="ISB30_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAP1989954.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            16438..17847
                     /gene="glnA"
                     /locus_tag="ISB30_002326"
     CDS             16438..17847
                     /gene="glnA"
                     /locus_tag="ISB30_002326"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAP1989955.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            18021..19070
                     /locus_tag="ISB30_002327"
     CDS             18021..19070
                     /locus_tag="ISB30_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HAP1989956.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            19082..20491
                     /gene="glnG"
                     /locus_tag="ISB30_002328"
     CDS             19082..20491
                     /gene="glnG"
                     /locus_tag="ISB30_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAP1989957.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESNVPE
                     STSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            20603..20713
                     /gene="yshB"
                     /locus_tag="ISB30_002329"
     CDS             20603..20713
                     /gene="yshB"
                     /locus_tag="ISB30_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAP1989958.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(20946..22319)
                     /gene="hemN"
                     /locus_tag="ISB30_002330"
     CDS             complement(20946..22319)
                     /gene="hemN"
                     /locus_tag="ISB30_002330"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP1989959.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(22508..23017)
                     /gene="yihI"
                     /locus_tag="ISB30_002331"
     CDS             complement(22508..23017)
                     /gene="yihI"
                     /locus_tag="ISB30_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAP1989960.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            23599..24231
                     /locus_tag="ISB30_002332"
     CDS             23599..24231
                     /locus_tag="ISB30_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP1989961.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(24613..27399)
                     /gene="polA"
                     /locus_tag="ISB30_002333"
     CDS             complement(24613..27399)
                     /gene="polA"
                     /locus_tag="ISB30_002333"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP1989962.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         6807 a         7217 c         6785 g         6713 t
ORIGIN      
        1 atcaggatgg tgtacccgaa aaagtcagca gctttaccgc accaggggtt aaggtgaaat
       61 ctaccattca taacgaccgg tcagtgccgc accaaccggt ctttaattgt tagtagtagt
      121 aaatttgtgg ataacagtcc gggaaccaga cgattccttc cggatcatcc ccctgcatta
      181 cccgatgaca ttggcgtcga tcatctgcgc gacggcgaag cgtagtatag agccgcttcc
      241 attcatgatc gcccggtgta ctgtagttta cccaacaatc cattgaaggg tgacgccatt
      301 tacctgtaag tgttgggatc cctgaatgct tatgcggctt cgctttacca ttacgatccc
      361 tatctttacg cgtattaaac gtggacattt ttagtctcct gttaagagtt ctaaaaatgt
      421 gcttccttaa aatagttcat cattttccct taattcgcat agcgtgcttt tgcacgaatt
      481 tccgatacgc ggggaccatt atgatagcgt cgatgcccac acatccaaca agaacaaata
      541 cagggagtac gaaagcaaat acctgatgct tttttgcttc catcgcctgc agtgcgataa
      601 cgacagcgga ttttttttaa tcgcaccata tgatggcgac gttcagcacg attacgggtc
      661 atagcaaaac tcctgaacag gcggcactat gccgctttca taggagttct gaatgctggt
      721 gctttctgta tggcataacc atgcctctaa cggttaagtg cttgcagcac atgttgcgga
      781 tcattggcaa tcgctcgcag caaagccttt gcaggcccgg tcggtgaacg tcgcccctgc
      841 tcccagttac gcaacgtgtc tacgcttacg gaaataagat cggcaaattt agattgtgac
      901 aaccctgaag cgcggcgtat ttctttgatt ttcatagcat caacttcaaa agtccgtgaa
      961 ggctgtcgcg ttccttcaat aatttcattc atctgggcca tgctgtgagt caaccgctca
     1021 aaaagtgcct tatccattta ccacctcgta tttaacaacc ggagcattgc tttttcctgg
     1081 ggagacagat catctttaat acccttacga taaataagca gtaatctgat ctcataatgt
     1141 ttaacctgat gaaagtaaat aaccctgaca ccagcacgtt tcccctttcc tcctgagacc
     1201 catcgaacct ttcttaaccc accagtttca ggtattacct caccatagtc aggattcaag
     1261 gccaggaaaa actgaaaacg actgaattca tcgtcgttca gcagtttttg aacatcttca
     1321 gtaaaaattt ccgtttcgat gaagagcata attatacgcc attggcttag agcaacaatc
     1381 ctttttgtac gtcattgacg taccattgaa accactagct tactgatagt gctccagcaa
     1441 aaccacgtct ctgccgggtt tgtctttaca tttgcgaggc gctttccaga tttttcacgc
     1501 ccgtgaattt ccctgtatcc atccagcaaa aacgggtgta taaaactgta cattcataca
     1561 gcaaaatgag ttctactatg gcactggaaa aaggaattgc agagttagtc gaagagttta
     1621 tcgctgcggg tcgcccctca tcgcgccaac aaagtattac gcagcgcaga gagggctata
     1681 tcgccagcgc tgtccttgcg ggtgagacag aaacgcgggt agatattcag actatcgaac
     1741 ttgaagggat gaccttacga atagtgtcac cgctgaatgc cccgacatta ctcccgacga
     1801 taatttatta ctacggtggc tgttttgtta gcggcgggtt cgcaactcac gacaaccagc
     1861 tgcgccagtt agcctattac gggcaatgtc gggttattgc cgtccagtat cggctggcac
     1921 cggaacatac tttccccgcc gcccatgatg atgcacaacg aggtgcagaa ttagtccggc
     1981 aacacgccga acgactcggc gttgataaac aacgaataac tcttgccggt gacagtgcgg
     2041 gagggcatct ggcgctggta actgctttgc gattgaagcg tgctggcgag tggcaaccag
     2101 cacaactcat cttaatttat cctatgcttg atgcgactgc gcatttcgaa agctacatac
     2161 gcaatggtca cgattacatc atcacacgcg acactttact gagcggcttt gagatgtacc
     2221 tgccaggcat tgaacgcagg catcctgaag ccagccctat ttggcgcaat gatttcaatg
     2281 gcttgccacc tgtacatatc attaccgctg aatacgaccc actttgcgat gaaggtgaag
     2341 cgttgtatca ccgcatgaca gggcaaggag tgcaatgcac ctgccaacgc taccttggcg
     2401 ttatccacgg attttttcag ttaggtggta tcagtgaggc tgcgcgatcg gcgttacggg
     2461 atgttgcctg gagagcggga aggtaactcc ggaccagggc ccagaaaagg ttttcagatt
     2521 gaaaaccttt tcagtcaagt aaaacgcccc tactcttctt cgttcccgcc ctcttcatac
     2581 gggccaaatg gcttcgcggg cagaacgata tacgtgcctt caaacactgc acccggcgtc
     2641 tcgtcgccaa agatttctac ttgcatctgc acccgtgctt ttcgtccgcg cgccagacgg
     2701 tcgagatcgc cacttaaggc accaaggtcg gctaccgcat ggggttttcc gctaatcggc
     2761 ttgctgtagc ggatatgcgc atccgccaga ataatcgttc cgccgaggtg gcgttcgcgc
     2821 agcatcagcc agataagccc ccaaccggtg agcgtagcca gcgagaataa actcccggca
     2881 aacagcgtgt gatgcggatt ctgattgccg gtttccggca tggtggtgat aaatttttgc
     2941 ccggtatatt gctgaatgcg cacacccatt ttttcgctta acgggatatg ttcataccac
     3001 gcttgctgga gctgggcgca ccagtcgccg cgatgaagaa tatcatccag agtggcgacg
     3061 ggtttaatca tcagaaaatg gcgaatcggc gtggtggttg gcgtggtgat ttcaccctga
     3121 ttaacaaacc ccagcttggc gaaaaactcc accgcgtctt cacgggcgct acaggtcacg
     3181 cgcttaacgc cttcctgacg tgccaccgac tccagggtca tcgccatcag cgtgcctaac
     3241 cctttgtcct gcacgtcggg atgaacggcc ataaagcgaa tggacgcttc attgtcggca
     3301 ttaatataca gtcggcctac cgctaccaga ttaccctgct cgtcgacgac catctggtga
     3361 tgcgccatcg catcccacgc gtcgcgttcc gaaccttttg gttgatgcag gggcttacgc
     3421 aacatttccc agcgaaactg atagtaacgc tctaattctt cttctgtttg tggaacccga
     3481 aggtgataca tagcgatact ctctcttgtt tcccgtgaca accctggaag ctggctcata
     3541 cctgcaacca gaatgtcacg gggccatcat tgaccagcga tacctgcata tccgcagcga
     3601 agcgtcctgt ttgcgtgttc atatcttgct gacggcagcg ttcgacgaaa tagtcatata
     3661 acgcctctgc gcgatccggt gatgcgcctt tggagaaact tgggcgcatc ccccgttcgg
     3721 tatctgcggc aagggtaaac tgggaaacca ccagtacact gccgcccgcc tgttgcacgt
     3781 tgagattcat cttgccttcg gcatcgctaa agatgcggta gccgagtaca cgctcgcaca
     3841 gacggtttgc tttctgttcg tcgtcatcct tttcgacacc caataacacc aaaagtcccg
     3901 cgccaatttc acccgtcact tctccctcca cggtgacgct ggcacgggtt acgcgttgaa
     3961 ttaatgcaat catggttcgt cgtcttcttc ttgttcagca gcttgtttta atttgcggta
     4021 ttccccgaga gtgacagtaa tttccgcgcc aagcaagacg atacaccatg tccagtagac
     4081 ccaaacaaag agaatgggga tcaccgccag cacgccgtaa atgagctgat atgacgggaa
     4141 catggtgata taaagcgcga aacctttctt tcctgcttcg aacaggagcg cggcgacaaa
     4201 cgcgccgaca atcgcgtcgc ggttaggtac gcgaatggta ggaacgatgc tgtacagcaa
     4261 ccaaaaggag atccacgata acagcagcgg aaaaatacgc agcacgttgt cgatgacagt
     4321 attgagatcg ctcgcccagc gcagggaaag cagataggag ctgatcgcca gactggcccc
     4381 tgccagcagc ggccccagcg ttaaaatcat ccagtacacg gcaaacgagt aaattttggg
     4441 tcgcgctcgt ttactgcgcc agatggtatt caacgcgcta tcgatggagt acatcaacaa
     4501 taacgccgtg acgatcagcc cgcacgcccc gacggcggtc attttgttgg aattggcgac
     4561 aaactgttcg atatatcgct ggataacatc gccagtagca ggcagaaagt tggcaaaaat
     4621 aaagtgacgc aactgaatgc tgacgtcgga aaacatggga aaagcggcga aaagcgcaaa
     4681 aacaacggca accagcggca ctaatgagag caacgacaca taggcaaggt tacctgccag
     4741 ggttgtcatg ttgtcctcat caatgcgttg ccagagtagt tttagccagg cccatagtgg
     4801 acgggtacga tgcctggctt tgtcctgaat ggtttttagc ataacacctt cgcgaaatag
     4861 tccgggatgg tggttttatc tttcaccaga atactagtaa tgcctagccg attagctcct
     4921 tctatattat cggcgttatc gtcgaaaaag accgtatcgt ctggtgaaaa accttctgcc
     4981 cgcaaaacat gctgataaat tcgtgcttca ggtttgcgca tacccaagtc ttgcgacaga
     5041 tagatatggt cagcagcatc acggatttcc gggtattcct ccggccagaa ggtagtatgc
     5101 aggcggttag tattggaaag caccaccacg cgatgcccct gctcacgcag tttatgcatg
     5161 atggcgatca cttccggtcg tagcgcaaca aataccgcct gccaaccgtg ggagaactgc
     5221 tcatagctta gcggcagggc catctcatga cacagtgcct ctgcgaacgc ttcgtcgcta
     5281 atttccccac gctcatgctg atgaaacgcc tcgcccatgt ggaaactctt cttaagcgta
     5341 gccagtggaa cacgcgttaa atcgctccag gcccccagca cacggttaaa gtcgatatcg
     5401 acaatcacat tacctaaatc aaagatatag agcatttttg cctcctttcg cgccacaaga
     5461 aattaactgt agcgggaaag gatgggtttg gctatgtggc aataagggta atcttgtgat
     5521 gctgattgcg aaaaagcaga gtaatttcac tggctttttc gcaatagatg aatggttaag
     5581 attcaggtgt aaattgccaa cgagtttcga tgaccagcgg cttatctggt gtcacaaatt
     5641 ccggcgttag atttattgcg ttaggtgcct gagtcagcgg gttaatacag gtggcatcgg
     5701 gctgtttatc aaataccacc agactgtttg cagaagaggt catggtcatc gcgagtttac
     5761 caggccacag taacttaacc ttcaccccgt cataaaaacc aaaacagtca tcccagggac
     5821 caacttgagg agagatacga tttccagttg gcagttcatc actacccgct tcttcttgcc
     5881 agtccgcatc aaaaagcacc tgcaacgatt ctgtattttg cggagtcagc tttttcgcaa
     5941 accacggatg ccagcctgcg gaggccggga atgtatcggc atatgaatgg acttccagtt
     6001 gcaaaaccag cgcatcgttc tccagcaaaa aagtctgaat cacttcccct tgccagggcc
     6061 acggtgaagt caggggcata cgtaaagtca gggaatctgc tgttttgtcg atgatttccc
     6121 acgttgagta acaagccata ccatgcaacg catgtggtgg tttattggcg ggtaaagaat
     6181 aacattgccc accagcattc agcgtcgcat tccccagtcg ccctgcccac ggcaccattg
     6241 ggaaacagcc atactgaaaa gctctgcgct gcggctgcca ttggcgtaat acttcagaac
     6301 caaatgcctt taacgaggtt atcctggcac catcatgcgg ataaatctgc aggtgaatat
     6361 tgcctttttc cagcgtcact aactctccta caggcccagg ccatggcgcg tcaaacgcct
     6421 gttgtagcgc caggtaatgc tcgttttttc gttcagtttt catgagattg ttccctgatg
     6481 tagcggtaag aagcggtttc ttgttcatcg gttggctcaa tcagccagac gcaaacaaag
     6541 gaaagaactg agacagcaaa taaaaacaga gcgacatacc acggttttcc tccccctagc
     6601 gccagcaaac ttgaggcgat aagggggctt agtccgccaa gaatcgcgga aaattgataa
     6661 gcaaaagaaa ggccggtata tcgtactttg gtaccgaaca tgcgggtgaa aagtgttggc
     6721 tgcacggcaa acatcatcgt cggacctaag ttgtagccaa ggatcatact gcaccagatg
     6781 atgagcgtac ttttgctatc cagtaataag aagaaaggaa acgcgaacag gacacaaaac
     6841 cctgctcccg acaaatacag acttttttga cctatacgat ctgataacca tccaaccaat
     6901 ggacaactga aaataccgat cgccgaggca atcaacatac ccgtcagggg aatatctcgc
     6961 gacaatgcta attggctgga aatatagaca ataccaaagg cgttaataat attagaggag
     7021 acactttccg ccagcctcgc acccagtgcc aataaaatat ttcgtggatg ttttttgaat
     7081 aattcaatca acggaggaac ggacttttct ttattattcg tcgttttttg cttttgaaaa
     7141 tccagcgttt cttcggtatg caggcggata aacagcccaa cgatcaacat caccgcagaa
     7201 agccaaaacg gaatgcgcca tccccaggag agaaactgct ccgaagtaag aaaatggtta
     7261 cagagagcaa aaataccggt tgccagcata atacccacag acgcagccgt ttgcgggagt
     7321 gaacctaaaa atccgcgtcg ggattcgggc gctgattcta tggtcatcaa tgccgctccg
     7381 ccgtactcgc caccaagacc aaagccctga attaaacgta gcaccattaa tatgagcggt
     7441 gcccagatcc caatttcctg atacgtcgga ataaaaccaa tcaaaaatgt ggagcaaccg
     7501 acaatagcca acgtccagat aagcgttatt tttcgcccta ttttatcgcc aaaatgacca
     7561 aagacgatgc cacctaaagg acgagtcaga aaaccgacac caaaggtcgc aaaggaagct
     7621 ataagaccta taaaaggatc gctgtctgga taaaacagcg gtgcaaatac cagtcctgct
     7681 gccgtgccaa aaataaagaa gtcataccat tccattaacg cgcctgcgaa actgcccata
     7741 acgacacgtc tcatggcttt accggaaagc ccttcacgcg gtgcgggtaa cttttcagac
     7801 ataattttcc tctcctggta ggcgagaaat ctgggatacg aaccactaaa cgtgggcagt
     7861 ttgaattaat gcgcatcggc ttaaaccgat gcgcattaaa ttatttcttc aattcagcca
     7921 gaacctctgc gtcctcagca tcaacatttg cacccagtgg ttcccaatta atcaatacac
     7981 aggtcaggaa tcccaggaat ggcacgattg ccaaccagta aaacgcttcg gttttcaacg
     8041 caccccacag catcggccag aaaatgagtc ctgcaattgc ccctgtgcgc ataatagcgc
     8101 gggcaaatcc gaccccagta gggcgaatag cgggaggata agagagtgtg gcaatggtca
     8161 tgcccagccc accaggaccc gctgaatgga agaaaataat tgatcccaat aaccctaaag
     8221 acagccaggg attagtcgtt gccagtgcgc ctaacgacag caatgctaag gtcacaacag
     8281 caaatccata catcgactgt cggcgagtac ccagtcgttg gagaataaac gagccgatca
     8341 tcccgcctgt caccccacaa agggcattca cgacggcaga accggtcaac gcgccagtaa
     8401 gcccgccact tattattccc gccaacgtaa gaggaagata aacacctacg gcgttatatt
     8461 gccacgcctg catggttgcc acgacacagc caagaatggt gcgtttgcgg taacgatcat
     8521 taaataagat gccatatcca ccactgtatt tattctctgc tttttccgag aatttcgctt
     8581 ctgttgtacc cgatgccgca acgcgagcct gaacaccata acgttttccc agaatgtcgc
     8641 acgcttcctg gtaacgcccg acgcgtgcgg cccacattgc ggattcacca ataaagaagt
     8701 aacgcaaaat catgaagatg actgcaaaaa tagctccgat gaatattccg taacgccaca
     8761 attgttcttc agcgataccc gacagaagca gcggcaaaag caccaaataa acaacggttg
     8821 tcgaaacata ccaggccatt tgccagaggt tgaccgacgt tccggttttc ttcgacgttg
     8881 aaccacgaag ctctgccacg gcattggttg ccaaagggaa gtcgatgcct aaaccaaagc
     8941 ccatgatgac gcggcacacc agcacccacc agatactggg agcaatagct accgctgcag
     9001 caccgatggt gcacagcccc atgccaataa taaatgcccg cttttgccca aagcggttta
     9061 ttactcggtt cgctaataaa ccgccaatta acgcggcgat taatactgat gcattgacgg
     9121 ttgcagcaag acccggcgaa atgccgaatt gagcggcaat gtatttatta ccaaacccca
     9181 ccatcgccgc ctggtaggca tcaatcaata tgccgcccaa agcaataaaa ataacgccga
     9241 tactggtgtt gattgcagag ttcttattaa caatatcaat tacgtcctgt ggccgactaa
     9301 ttgaataagt tgcaggactg gtgatttgac tcatcgcagt gtccttttta attgttgtag
     9361 ggtggcgctg acgtgacgca gcgccagaca gagatttaga aaatcattcc ggcgcaaatt
     9421 gcagcatgac ctttatggag tcattttgct gcgcgcgttc gaatgccgcc gctgcatcct
     9481 caatggcaaa taaatcggta accagttcac tggtatcaaa ccgcccatcc gccagccatt
     9541 cgataacagc atcgaaatct tcagggatgt acatgccaga gttgagcaag tcacgctcga
     9601 aacgttgcat catcggtaat ggtatttcac gcggcccact tggtacgccc atgcacacga
     9661 cggtgcctcc ggcatataca cgggtacatg catcgttaag cgtggcctgc gctgcaacga
     9721 catcaatcgc ccccgtaaat cgcacatctg cggccagctc acctggtgcc caaacttctg
     9781 ctgcgcccag ttttaacgcc agcgcgcgtt tggcagcgtc aggttcaatc acggtaatct
     9841 tacctgcccc cataatgcgc agtgcctgca cgatggataa gcctatcgtt ccggcaccaa
     9901 tcaccagtac gctttccaga gaagctttcg gcattcggtt aacgcagtga cgagcacaag
     9961 ccgccggttc ggcaaaggcc aacactttta gcggtaaaga ggctggtgcg acatggcaat
    10021 tacgcgcagg aacaatgtgt tgtgagcgag caaagcccgg tgcgcgaaaa ccagcaacct
    10081 gcggtggttc acaaagatta aaacgtcctg ctttgcaggc tcggcattcc atgcaagcca
    10141 tgatgggatc gaccacaaca tgatcgcccg gctgtacatt tttaacgtcg ctgcccactt
    10201 ccgtaacgcg cgctgcaatt tcatgaccgg ggacgacagg cggtttggca aacggatgac
    10261 cgcctttcag aacatgcaga tccgaaccgc atatcccgta gaacacgggc gcaatccgta
    10321 cttcgtgcgg tttaagttgc tggtgtggta cttcaacata ttgagtggtg actttcccaa
    10381 tatctgaaaa taaaacctgg gtaattttat ccattgttat tctccagaat cgatatccat
    10441 aggggattag cgttgcgcga taccttcatc gacaatttca gccagccgtt gcaagcgtgg
    10501 aacggagaca tcgcgcagca tggtttgtgg atcttgtgca ccaacaaccg acgcccaaac
    10561 cgctcgtcca gccaggaagc ccgacgcacc acctttcatg gcaatactta ctgcacggcc
    10621 aaaaatatcg gcatcaacgc cagaagaaag aattacccac ggcatcttca tctgatcgtt
    10681 aagttgctga caggcagcaa gtaaggtttt ttcatcgcct ttgcccccta atggcatctc
    10741 ggctttgtaa agatcagcct ctgttccacc aagctctgcg gcggcggcaa caatggcgct
    10801 ttcacgatca aagtcccaac cgcgacgagg tggacgcacc acaggttcaa taatgctaac
    10861 cagcccggca ctacggcagc gacgaacaaa tttatcaacc attgccagac gttcctctgc
    10921 gggttcatct tcacgccaga gtacaagtaa tttcattgcc ttagccccca tttcacgggc
    10981 tttatgcggg tcgacggaca tatctatttc aacgctatca accgggatgc cattacctgg
    11041 gataaaaaga tcggcagcga caatcaggcc gcaagaatta gccacagcgc cctgctcgac
    11101 tatttgttca agacaaaatt gtttatccgc cagaacggca gaagcataag gagaaaggat
    11161 tcgggtcgcg gcaactttaa aatcggtgag tacactgtcg gcgatgggtt ttggctgccc
    11221 tgctgccgca aacatcaggc gcatggcctc acgctgatcg acagctaaca tggcgaatcc
    11281 accacctggg cgggagatat ctttcagcgt aaatggggtg aagtgggatg acatagaaac
    11341 tcctgttaat agataaaatt aaagaccagc ctgctggcga acctgattta gcagggcgct
    11401 ccagtcctgc cgccctttgc cgtcttgact tgctgcggcg taataacttt ctgctgcaat
    11461 ccccatattg agcggcgccc ccgttttccg agcgacgtca actgcaatac gcagatcttt
    11521 caatgccagg tccaccatga aggcgggaga aagatcgcct ttcagcactt tgccaggcca
    11581 ggtggtcgta aaatgtcctt tccctgctgc cgtaccactc atcaccttaa ttgcgacatc
    11641 aagatttaat cccagtgact cacataacac agcggcttcg gaggacagcg cattaagcgc
    11701 aatgctcata tagttgttaa ttagcttcac gcggatcccc attcctgggc caccagcctc
    11761 gaccagctca ttacccatgc acatgagaat cgggcgtgca cgcgcaatct gctcatcagt
    11821 gccgcccgct aaaatcagca atgtgccatc aatcgcattc actgacgttc tgcccacggg
    11881 ggcgtccatc atattgatgc ctttttcttg taattcccgt attaatgcat cggtctgtaa
    11941 gggatgaata gtcgacatat caatcactaa cgcgtcagat gaaacagttt cacagacgcc
    12001 tttttccccc aacagcacct gtcgaacaat gtccccattg ggaagcatag tgatgataaa
    12061 ttcagcgctt gctgccgctt ccgctggggt ttgtgccgca gtggccccct gcgtaactag
    12121 cgcatcgacg gcctgtgtat tgacatcaaa tacctgtaat gaatgccctt tttgcaatag
    12181 attgttagcc attgggctac ccatctgccc cagcccgaga aatgcaatgg ttgccatgaa
    12241 tactctcctg tctgacattg acaaaataat gacaattgaa aattcgtttt cgtctgtttt
    12301 tgagcatatt tgtaatttat gttcaaaaac acgacacgca tcactaattt gatcatttgt
    12361 gtcatttaaa ttgaccaaag acgacaaaat taaacaggac aagacaaata tgaccagaat
    12421 tgcatgcgtg ggtatcacag ttcaggacag aatttattct ttgcccactc tgccggaggg
    12481 tggcgggaaa tatcaggcaa atcattatct tgagattggt ggcggcccgg cagcaacagc
    12541 cgctgttgca atcgcaaagc ttggcgtgga ggttgatttc atcggtcgcg tcggcgatga
    12601 cagttgtgga aataccttac ttgcagaact tgagggttgg ggcgtcaata ccgcattttg
    12661 ccgccgatat cctaacgccc gttcgtcgca atcagcgatc ctcgttgatc agcatggcga
    12721 acggattatc gttaattatc caagtcctga tctcggtact gatgccgaat ggttagaagc
    12781 gatcgatttc tctcgttatg atctgattct ggccgatgtt cgctggcata gcggtactga
    12841 aaaagcattc tcgctagcgc gccttgcggg tgtgacaacg cttttagacg ccgatatgac
    12901 accgcaggat atttctccac ttgtcgcgct tgccgatcac gccgtgttct caacaccagg
    12961 gctgaaacgc atgactggcc tgcagtcacc tgaagaaggt ttgttccagg caacgacaca
    13021 aacagcaggt aaagtgtatg ttacgctggg ttctgaagga tcgttgtgga ttgaagatgg
    13081 tcatttgtgt caacaggagg cattttctgt caatgtggtt gataccactg gagcaggcga
    13141 tgtttttcac ggcgcactcg cggtagcgtt ggcagaaaaa atgccaacaa aagaggctat
    13201 tcgttttgcc agtgcggttg ccgcgatgaa atgtacccag cctggcggtc gggccggcat
    13261 tcctaatcgt gagcaaaccg aatcattttt gtcactttat gcgtaaaatg gtaaacatct
    13321 accgaggagg ggcaagggag tattcatgag tatcatcgaa gtgacaggta atccacgcca
    13381 tgaccaactc gttcatctaa tcgcagaacg cggttatatg aatattgaag aactggcaca
    13441 actgctggat gtttcaacgc aaaccgtgcg ccgggatatc cgaaaactca gtgagcaggg
    13501 cttgatcacc cgtcatcatg gaggggccgg gcgcgtctcc agcgtcatga ataccgcttt
    13561 tgagcaacgg gaactttcgc tcaccgccga gaaacgggcg atcgctgagg cagtcgccga
    13621 ttaccttccc gaacgctgta ccgtctttat caccatcggt acaaccgtag aagccgttgc
    13681 cagggcatta ctcaaccggc gtgatttacg cattatcact aatagcctgc gtgtggcaca
    13741 gattctttat aagaatcagg atattgaagt gatggtgccg ggaggcactt tacgcgctca
    13801 taacggtggg attatcggcc caggagccgt ggattttatt gaaggcttcc gcgcagatta
    13861 tttaatcacc agtataggtg ccattgaaca tgacggcacc ctactggaat ttgatgtcaa
    13921 tgaagcgtta gtcgcgagaa cgatgattaa acatgcgcgg aatacattgt tagtggccga
    13981 tcatacgaag tttaccgcgt ctgctgccgt ttcaattggc aatgcacgga atgtcagggc
    14041 tttttttact gatgccccgc ctcccaattc tttctgccag ttgttaagtg aagagaatgt
    14101 tgaactggtg gttgccgagc aagaagtatc ctgaaattga aacttcggaa aatccgctgg
    14161 gaatttcagc ggattttgtt ttccataagc caaaaaaaaa cctgccatcg ctggcaggtt
    14221 ttttatgact gaaaaacgaa attaatcgtc tttcggtgcg cggttagcgc gacgacgatc
    14281 gttttccgtc aggtgacgtt tacgaatacg gatagaggtc ggagtcactt ctaccagttc
    14341 gtcatcatcg atgaactcca gagcttgttc cagagtcatg cggataggcg gaaccagaac
    14401 aacggcttcg tcagtaccgg aagcacgcat gttggtcagt ttcttaccgg tcaggcagtt
    14461 tacagtcagg tcgttagagc ggctgtgaat accgataatc tgaccttcgt aaacttctgc
    14521 accgtgaccg aggaacagct taccgcggtc ctgcagaccg aacagtgcga acgcgaccgc
    14581 tttaccctga ccgttagaga tcagtacgcc gttctgacgc tggcccactt cacccggacg
    14641 tacgtcgtcg tagtggctga aggtggagta cagcagacca gtaccggaag tcatggtcat
    14701 gaactcagaa cggaagccaa tcagaccgcg gcttgggatc acgtagtcga gacgtacacg
    14761 gcctttaccg tctggattca tgtttttcag gtcgccttta cgttcgccca gcgcctgcat
    14821 tacagaaccc tgatgctgtt cttcaacgtc cagagtcacg ttttcatacg gctcttgttt
    14881 acgaccgtcg atttcacgga agataacttt cggacgggat accgccagtt cgaaaccttc
    14941 acgacgcatg ttttcgatca gaacagacag gtgcagttcg ccacgaccag aaacgcggaa
    15001 cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg ttgtgtacca gttctttgtt
    15061 cagacgatcc aggatctgac gagacgttac gaacttacct tctttaccgc agaacggcga
    15121 ggtgttaacg cagaagaaca tagaaacggt cggctcatca acggagagtg ccggcagcgc
    15181 ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc agttcgccca ggcccgtgat
    15241 cgccacgata tcgccagctt ccgccagatc ggtttcgata cgttccagac cgaggtggcc
    15301 cagcacttta ccgactttcg cgttgcgggt tttgccttcg ctatcgatga tagtaacctg
    15361 ttggttcggc ttcactttac cgcgcttgat gcggccaatg ccgataacgc caacatagct
    15421 gttgtaatcg agctgagaaa tctgcatctg gaacggaccg tcaaggtcaa cgtccggcgc
    15481 aggaacgtgg tcaacaatcg cctggtacag cggggtcatg tcttccgcca tatcttcgtg
    15541 gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg atcgggaagt ccagctgctc
    15601 gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga tccacaaccc aatcaggacg
    15661 cgcgccaggg cggtcaactt tgttgataac aacaatcggc ttcaggccgt aagcaaacgc
    15721 ttttttggtt acgaagcgcg tttgcggcat cgggccgtca aatgcgtcaa ccaccagcag
    15781 cactgagtct accatggaca ttacacgttc aacttcacca ccgaagtcgg cgtgccccgg
    15841 ggtatcaacg atgttgatac ggtaatcatt ccatttgata gcggtgtttt tcgcgaggat
    15901 ggtaatccca cgctctttct ccaaatcgtt ggagtccatc acgcgctctt gggtttcggc
    15961 acgagagtcg aacgtaccgg attgttggag cagcttgtct accagggtgg ttttaccatg
    16021 gtctacgtgc gcgatgatgg cgatattacg caatttttcg atcacaactt tgcctcaggc
    16081 attagaaata gcgcgttatt gtacacggat taatcgcact acaaaacagg atcacaaaca
    16141 tcctccgcaa acaagtattg cagagtccct ttgtgatcgc tttcacggag cataaaaagg
    16201 gttatccaaa ggtcattgca ccaatatggt gcttaatgtt cccattgaag cactatattg
    16261 gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg ttaagcatga taacgccttt
    16321 taggggcaat ttaaaagttg gcacagtttt cgctttatct tttttacggc gacacggccc
    16381 atttaattgc agatttcgtt accacgacga caatgaccaa tccaggagag ttaaagtatg
    16441 tccgctgaac acgtactgac gatgctgaac gagcacgaag tgaagtttgt tgacttgcgc
    16501 ttcaccgata ccaaaggtaa agaacagcac gtcactatcc ctgctcatca ggtgaatgcc
    16561 gaattcttcg aagaaggcaa aatgtttgac ggctcctcga ttggcggctg gaaaggcatt
    16621 aacgaatccg acatggtgct gatgccagac gcatccaccg cagtgattga cccgttcttc
    16681 gccgattcta ccctgattat ccgttgcgac atccttgaac ctggcaccct gcaaggctat
    16741 gaccgtgacc cgcgctctat tgcgaagcgc gctgaagatt acctgcgttc tactggcatt
    16801 gccgacaccg tactgttcgg gccagaacct gaattcttcc tgttcgatga catccgtttc
    16861 ggatcgtcta tctccggttc ccacgttgct atcgatgata tcgaaggtgc atggaactcc
    16921 tccacccaat atgaaggtgg taacaaaggt caccgtccgg cagtgaaagg cggttacttc
    16981 ccggttccgc cggtagactc agctcaggat atccgttctg aaatgtgcct ggtgatggaa
    17041 cagatgggtc tggtggttga agctcatcac cacgaagtcg ctaccgcagg tcagaacgaa
    17101 gtggctaccc gcttcaatac catgaccaaa aaagctgacg aaattcagat ctacaaatat
    17161 gtcgtgcaca acgtggcgca ccgtttcggt aagaccgcga cctttatgcc aaaaccgatg
    17221 ttcggtgata acggttccgg tatgcactgc cacatgtcgc tgtctaaaaa cggcgtcaac
    17281 ctgtttgctg gcgacaaata cgcaggtctg tctgaacagg cgctgtacta cattggcggc
    17341 gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc cgaccaccaa ctcttacaag
    17401 cgtctggtcc cgggctacga agcaccggta atgctggctt actctgcgcg taaccgttct
    17461 gcgtctatcc gtattccggt ggtttcttct ccgaaagcac gtcgtatcga agtacgtttc
    17521 ccggacccgg cggctaaccc gtacctgtgc tttgctgccc tgctgatggc cggtcttgat
    17581 ggcatcaaga acaagatcca tccgggcgaa gccatggaca aaaacctgta tgacctgccg
    17641 ccagaagaag cgaaagagat cccacaggtt gcaggctctc tggaagaagc actgaacgaa
    17701 ctggatctgg atcgcgagtt cctgaaagcc ggtggcgtgt tcacggatga agcgatcgat
    17761 gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc gtatgactcc gcatccggta
    17821 gagtttgagc tgtactacag cgtctaatag ttgaagttgt actacccggc gcaacaacgc
    17881 cgggatttag ttgccgtgga aactttcagc ccatctctgc atgggctttt ttctccgtca
    17941 attctctgat gcttcgcgct ttttatccgt aaaaagctat aatgcactaa aatggtgcaa
    18001 cctgttcagg agactgcttt atggcaacag gcacgcagcc cgatgctggg cagatcctta
    18061 actcgctgat taacagtatt ttgttaatcg atgacaacct ggcgatccat tacgccaacc
    18121 ccgccgcgca acaactgctc gcccaaagct cccgcaaatt gtttggtaca ccgttaccgg
    18181 aactgttgag ctacttctca ttaaatatcg agctgatgca agaaagtctg gaggcggggc
    18241 aaggttttac cgataacgaa gtgacgctgg tcatcgacgg gcgctcgcat atcctttctg
    18301 tgacggctca gcgtatgccg gacggcatga tcctgctgga gatggcaccg atggataacc
    18361 agcgccgctt aagtcaggaa cagctacagc acgcccagca ggttgctgcc cgtgatttag
    18421 tgcgcggcct ggcacatgag attaaaaatc cgcttggcgg tttacgtggc gcggcgcagc
    18481 tgctcagcaa agcattacct gatccgtcac tactcgaata taccaaagtg attatcgaac
    18541 aggcggaccg gctgcgaaat ctggtcgacc gtctgttggg gccgcagctg cccggtacgc
    18601 gcattaccga aagtattcac aaagtggctg aacgcgtggt gacgctggtg tcgatggaac
    18661 tgccgaacaa cgtgcggttg attcgggatt acgaccccag cctgccggaa ctggcgcacg
    18721 acccggatca aattgaacag gtcttgctga atattgtgcg caatgcgcta caggcgctgg
    18781 ggccggaggg tggtgaaatc attctacgta cccgcacagc gtttcaactg accttacacg
    18841 gcgagcgcta tcggctggcg gcgcggattg atgtggaaga taacgggccg ggcattccgc
    18901 ctcatttgca ggatacgctg ttttacccga tggtcagcgg ccgcgaaggt ggcaccgggc
    18961 ttggcttatc catcgcccgt aatttgattg atcagcattc aggcaaaatt gaatttacca
    19021 gttggccagg tcataccgag ttctcggttt acctgcctat caggaaataa aggtgacgtt
    19081 tatgcaacga gggatagtct gggtagtcga tgacgatagt tccatccgtt gggtgcttga
    19141 acgtgcgctc gctggagcgg gtttaacctg tacgacattt gagaacggcg cggaagtact
    19201 ggaggcgctg gcgagcaaaa cgccggatgt gctgctttca gatatccgta tgccgggaat
    19261 ggacgggctg gcgctgctca agcagattaa acagcgccat ccgatgcttc cggtcatcat
    19321 tatgaccgca cattccgatc tggatgctgc cgtcagcgcc tatcaacaag gggcatttga
    19381 ttatctgccc aaaccgtttg atatcgacga agccgtggcg ctggttgagc gcgccatcag
    19441 tcattaccag gaacagcagc agccgcgtaa tgttcagctt aacggcccaa cgaccgatat
    19501 catcggtgaa gcgccagcca tgcaggacgt gttccggatt atcggtcggc tttcgcgttc
    19561 ttctattagc gtgctgatta acggcgaatc cggcaccggt aaagaactgg tcgctcatgc
    19621 cctgcatcgc cacagtccgc gagccaaagc gccgtttatc gcgctcaata tggcggctat
    19681 cccgaaggat ttgatcgaat ccgaactgtt tggtcacgag aaaggcgcat ttaccggcgc
    19741 gaataccatt cgtcaggggc gttttgaaca ggctgatggc ggcacgttat ttctcgatga
    19801 aattggcgat atgccgctag atgtgcagac gcgtttgctg cgcgtgctgg cagacggtca
    19861 gttttatcgc gttggcggct atgcgccggt gaaagtagat gtgcggatta tcgctgccac
    19921 tcaccagaat cttgaacagc gggtgcagga aggcaagttc cgtgaggatc tgttccaccg
    19981 cctgaacgtc atccgcgttc atctgccgcc gctgcgcgaa cgtcgggaag atattccccg
    20041 tctggcgcgc cattttttac aggttgccgc gcgagagctg ggcgtagaag cgaagttgct
    20101 gcatccggaa accgaagccg cgctgacgcg tctggcgtgg ccaggcaacg tgcgccagct
    20161 ggaaaacacc tgtcgctggc taacggtgat ggctgccggg caggaagtgt tgattcagga
    20221 tttgcctggc gaactgtttg aatcaaacgt accggaaagc acttcacaca tgcagccgga
    20281 cagttgggca acgctgttag cacagtgggc agacagagcg ctgcgttccg gtcatcaaaa
    20341 cctgctttcc gaagcacagc cagagctgga acggacgtta ctgaccaccg cgctgcgaca
    20401 tacgcagggg cataaacagg aagcggcgcg gctacttggc tggggtcgca acaccctgac
    20461 gcgtaagtta aaagagctgg ggatggagtg attcacggct tgtgtgtaaa gattgattat
    20521 tgagcgcaaa ttgctggtat tttgcgcttt actgttccga taagttcagt atgatcttgc
    20581 ccggaaaacg gggagagtca ttatgctgga atcaataatt aatctggtat cgagtggcgc
    20641 agttgacagc cacacaccgc aaactgctgt tgctgccgtg ctgtgtgccg cgatgatagg
    20701 gctgtttagc tgaagctcgt tgtttacgcc ggatgcggag aaagtcgtag gcctgataag
    20761 acgcaaccag cgtcgcatca ggcgttgatt gccggatgcg gcgtaaacgc cttatccggc
    20821 ctacaaaagc aataatttca acagattttg caggcctgat aagcgtagcg catcaggcaa
    20881 tcttgttctc agcacattga tggcgctttc gttttctact ttgtaaacga agcgccattc
    20941 acaatttaaa tcacccgaga gaactgctgc atccgcgctt tctggcgcag ataggtatca
    21001 aagcacatgc aaatgttgcg gatcagcaag cgacctttcg ccgtcacctg tattcccttc
    21061 tcatccacat ccaccagccc atcttttgct aacggggcga gcagcttgag atcttccgca
    21121 aagtaatcag cgaagagcaa atcccactgt tgctcaatag gggcgtaatc cagacggaag
    21181 ttgcagatga gcgacttaat cacatcgcgg cgaatacagt catcacgcgt tagcgcaata
    21241 ccacgccaca gcgcattgcc ttgttcatcc acttgctgat agtactgctt caactctttc
    21301 tggttctgcg cgtagcagtc gccaatcatg ctgatggcgg aaacgcccat ccccagcaga
    21361 tcggtatcgc cctgagtggt gtagccctgg aagttacgat gcagcacgcc ttcacgctgg
    21421 gccaccgcca gctcgtcatc cggacgggca aagtgatcca taccgataaa ctgatagccc
    21481 gattgtgtca ggaaggcgat ggtttcctgc aggatatcga gtttttgctg cggactcggc
    21541 aggtcagcat ctttgatttt gcgctgggcg gcaaaaatgg tcggcaaatg cgcgtagtta
    21601 aagacgctca gacggtcagg gttcagttcc gccacacgct tcagggtaaa ggcgaaactc
    21661 tccggcgtct gtttcggcag gccgtaaatc aggtcaatgt tggtggaggt aaagccaatc
    21721 tcacgcgcat ggttaagcag tgcaaagatg aactcttcat cctgctcgcg gttaaccaga
    21781 cgttgcactt ctttgttgaa gtcctgcacg cccatgctca gccgattaaa gccttcggcg
    21841 cgtaaatgat cgagtacatc cagttcgatt tcccgcggat cgacttcgat cgagatctcc
    21901 gcatcggcat tgaactggaa gttttcgcgc agcagcttca tcagacggct gatttgcgct
    21961 ttattcagat acgtcggcgt tccgccgccc cagtgcaatt ggctgacgtg acgcccggcg
    22021 aacagcggtg cacgatggac gatttcttgc tccagcgcgt ccagatactg atcggccttg
    22081 tgctgctggc gagtaacaat cttattgcaa ccgcagaagt aacaaagctt atggcagaac
    22141 ggaatatgta catagagaga taatggacgc tcaggatagc gcgccacagc ttgtaaaaac
    22201 gcctgttcgc cgaagtcttc tgaaaactcc agcgcggtcg ggtacgaggt gtatcgtggc
    22261 ccggaatagt tatatttctg gatcagggcc agatcccagt cgatttgctg tacagacatg
    22321 ctcactcctt ccgatggcgt ctctggcggc tacggcgtac cggcggatgc ccgtttcgag
    22381 tcatcagccg gttgcgcagc cacttttgtc gccgcgacaa ccgctgtagt ttaacgaata
    22441 accacaccag ataacatata accggaaggg ttatcagcag aacgggaagg cccacggcgg
    22501 caatccgtta gttgccccgc agcagacgca tcatgtcttc ttgcttctcg tcttcttcct
    22561 cttcttcgtc atcgtcataa gagaggccga gtttctgcat caactcatca atacgatcca
    22621 gtttggcatc cacccaggat tgctcttcgg cgctcagggt ttcgcctgct tccagacgtt
    22681 ccagcagcgc atccagacgc tcatccgttt ccagtaactc caactccgcc tgcggtgaaa
    22741 gcataggttt ctcactcttc ggtttgtgct gtttggtgac tttttcagtc acgcccaatg
    22801 gaataggggt tttactgcca atacgtggat cttttggtgc gttctgacct ttgctgcctg
    22861 acgtggtgtt accgcccgct gcgcggctgc ccggcgcatg accacgacgt tttttctgac
    22921 gcttacggtc acgagcttcc tgatccagct cctcgcgtgt ttttcgacgc gcttttgcat
    22981 gacctttgct gcgtgagttt gaagatgatg gtttcataat ggttcgtctt ttagccgttt
    23041 tattcagtat agatttgcgg cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag
    23101 attactctgt cgcctttttt cctgactcat aacccttaac gggtcttaca atccttgcag
    23161 gccgccaaca caccctgttt ttccattagc cggaacaatc cgtgttgatt ccatttccat
    23221 ttaattacgt ctccggacgt ttatcttcct gacaatcctc tgtcttcgcc tcctggcgct
    23281 cctgaccctc atcctgagtc attgttcctg ttagcgtcct cgctctatgg gtgctacttt
    23341 acgcctttgc tttaaataaa caatcaaccg ccatcagtat ttgcacattt tcagagtttt
    23401 atataaaagc aattcactga tttatatagc ttatttgaca tacgtcgcag aattttctct
    23461 gctatttcgc ataaagacta tcggcaatgt ctcacaaagc atatggcttt tacttacaac
    23521 gctcacgccg aaaaatccgc cttttattat ggttcaggta taatcgccgc aaagctttga
    23581 ctgatggaga cgaccgcttt gactaatttg aattatcaac agacgcattt tgtgatgagt
    23641 gcgcctgata ttcgccacct accttccgat accggaattg aagtggcttt tgcaggccgt
    23701 tccaacgcag gtaaatccag cgcgctgaac acgctgacta accagaaaag cctggctcgt
    23761 acctcaaaaa ccccagggcg cacccagctt atcaacctgt ttgaagtggc tgacggcaag
    23821 cgtctggttg acttgcctgg gtacggttat gcggaagtcc cggaagagat gaagcgcaaa
    23881 tggcagcgcg cgctcggcga gtacctcgaa aaacgtcaga gcctgcaagg tctggtggtg
    23941 ctaatggata ttcgccatcc gctgaaagat ttggatcagc agatgattga gtgggcggta
    24001 gacagcaata tcgccgtgct ggtactgctg accaaagcgg acaaactggc aagcggcgca
    24061 cgtaaagcgc aattgaatat ggtgcgtgaa gcggtgctgg cgtttaacgg tgatgtgcag
    24121 gttgaaacgt tttcttcgct gaagaaacaa ggcgtggaca agctgcggca gaaactggat
    24181 acctggttta gcgagatgca gcctgtagaa gaaacgcagg acggcgaata attttcttgc
    24241 cttaatgctt gtgccggatg tggcgtatcc ggcccgtaaa ttcaatagat tacaattcta
    24301 ccgttggcct gataaacagg gcgcatcagg cattacggat cttttctttc gcccaataaa
    24361 aaacgcccca gtcattactg actggggcgg ctaaaatatt cagccaaatc cgattacgtg
    24421 aagtaaaagg tctgaaagat agaacatctt acctctgtac cctacgcgac taactttact
    24481 cttttttgtt cagttcagaa agcatttttt gtaatttttt ttcagttgtt gcataggaat
    24541 ttctaatagc catcacaaaa cgtgacagct tatgttgctt acttacgaaa aaagggcatg
    24601 ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca ctccccactt ccaccagcaa
    24661 cggcacatcc agacgggtac agttttccat cagttgatga atctgcttcg cgacggcatc
    24721 gacatcatct ttatggactt caaataccag ttcatcgtgt acctgcatga tcatacgcac
    24781 acgcggttgc tcagcctgta gccacgcatc aacggcaatc atcgcccgtt tgataatgtc
    24841 ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc
    24901 cccattgctg gatttgatat ccggcagata cagacggcgt ccgtccagcg tttcaacgta
    24961 gccctgctct ttcgcctgag cacgggtgcg ttccatatac tccagcacgc cagggtagcg
    25021 ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt ggaatgttca attgccgcgc
    25081 cagaccgaaa gcactcatgc cataaatcag accaaagttg atcgctttcg cgctacgacg
    25141 ttgctcgctg gtgacggttt ccagtggcaa accaaacact tctgccgccg ttgcacggtg
    25201 gatatctttt ccttccgcga atgcggtcag caagcctttg tcacgcgaga gatgcgccat
    25261 aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc acataatcct ctggcgcaat
    25321 aaacgcctgg cggatacggc gaccttcttc gttacgcacc ggaatgtttt gcaggttagg
    25381 atcggttgac gataaacgtc ccgttgcggt tactgcctgg tgataagagg tatgtacacg
    25441 cccggttttc gggttgatca tcaacggcag cttgtcggtg taggtcgatt tcagcttcgc
    25501 cagaccacga tattccagaa tcacttttgg caacggatag tccagcgcca gttcttccag
    25561 tacctcttcc gacgttgacg gcgcgccacc cggagttttc ttcagcggtt taatgccctg
    25621 tttttcaaac agaatggttt gtaactgctt ggtggaagaa aggttaaatt cttcacctgc
    25681 aatttcatgc gctttctttt ccagctcagc cagacgcaag gtgagctctt cagagtgatt
    25741 gtgcagcact ttcggatcga tcttcacacc gttacgttca atgcgtgaaa gcaccggcac
    25801 cagcggcatt tcgatattct cgaagacgtt caacggccct ttgtgttttt gcagatccgg
    25861 ccacattttc agatgcaact gcaaggtgac atctgcatct tcggcggcgt aacgtccggc
    25921 ttcttcgagg gcaatctggt taaaggtcag ttgatttttt cctttaccag caatctcttc
    25981 aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat cgtgacgccc
    26041 ggcaacgcta ttgagaatgt aggactccag catggtatca aacgcaatcc cacgcagctc
    26101 aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg ttttgcccga ccttcagcgc
    26161 cttttcatct tccagcagcg gttttagcaa ctcgagtgca cgctcgcgag agatttgatc
    26221 gggcgcatca agataatcat gagcaaccgg aatatatgcc gctacgcctg gctcgatagc
    26281 aaaagaaagc ccgaccaggt tagcagagat gttatcaagg ctgtcggttt cggtatcaaa
    26341 tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt cttcatcaag
    26401 gatggtgacg tagttgtcat aagaaatcac cgttgccgtc acttctggcg cttcgtctgc
    26461 aacactggtt tcctgcggcc tggctgcggg tttcgcccct ttagcctgta gccatttgcc
    26521 cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc ctaacaactc
    26581 ttccgccgcc ggtggctgca cttccagttg ttcacaggtc agctccagtt caacgtcggt
    26641 tttaatcgtc gccagctggt atgagagata agcaacttct ttgttttgct cgagcttggc
    26701 tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt ttttctggct cggcatacag
    26761 cgtatccagc ccgccaagac cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg
    26821 tacgccaggg atgttatcgg aggagtcacc catcagcgcc aggaaatcga tgatcagttc
    26881 tggcggcacg ccgtacttat tcaccacctc ttccggtccg aggatggtat tcgtcatggt
    26941 gttgataagc gtaatatttg gcgtcaccag ctgcgccata tctttatcgc cagtgctgat
    27001 cagcaccgga cgcccggctt tttcggcttc gcgcgccaga gtaccgataa cgtcgtccgc
    27061 ttctacgcca gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg cgtgcagagg
    27121 ttcgatttgt gctcgcagat cgtccggcat tggcgggcga tgtgatttgt aatgttcaaa
    27181 cagttcatca cgaaaggttt ttcccttggc gtcaaagacc accgctgcat gcgtcggttt
    27241 atattgcatg atcagactgc gcagcatgtt gaggacacca tacatcgcac cggtcggctc
    27301 gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat aagatgaacc
    27361 atctacaagg ataagtggat tttgggggat ctgaaccata atgtccgtgc ctgtttatca
    27421 gattatgggt aaaggatgcc acagacagga tgaaaacatg agtttttcac cgcttttcgt
    27481 gaaaatcttt tgtagatctt ctggatcgct cgcaaatcgt ca
//
LOCUS       DACADF010000060        26954 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.060, whole genome shotgun sequence.
ACCESSION   DACADF010000060 DACADF010000000
VERSION     DACADF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.060"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            61..504
                     /gene="cmtB"
                     /locus_tag="ISB30_002334"
     CDS             61..504
                     /gene="cmtB"
                     /locus_tag="ISB30_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HAP1989963.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMASLLDKNYISENYIQ
                     SIKDSTISNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGKDEPIKLLIG
                     LSAADANSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            532..1920
                     /locus_tag="ISB30_002335"
     CDS             532..1920
                     /locus_tag="ISB30_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAP1989964.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLFCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFTESANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1935..3212
                     /locus_tag="ISB30_002336"
     CDS             1935..3212
                     /locus_tag="ISB30_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAP1989965.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVVVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPARVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHIVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3209..4174
                     /gene="yggF"
                     /locus_tag="ISB30_002337"
     CDS             3209..4174
                     /gene="yggF"
                     /locus_tag="ISB30_002337"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAP1989966.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4196..4705
                     /gene="fumE"
                     /locus_tag="ISB30_002338"
     CDS             4196..4705
                     /gene="fumE"
                     /locus_tag="ISB30_002338"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAP1989967.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPRFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4702..5415
                     /locus_tag="ISB30_002339"
     CDS             4702..5415
                     /locus_tag="ISB30_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAP1989968.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDVHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDVLHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(5387..6064)
                     /locus_tag="ISB30_002340"
     CDS             complement(5387..6064)
                     /locus_tag="ISB30_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAP1989969.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LILQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(6058..6735)
                     /locus_tag="ISB30_002341"
     CDS             complement(6058..6735)
                     /locus_tag="ISB30_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP1989970.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            complement(6723..7430)
                     /locus_tag="ISB30_002342"
     CDS             complement(6723..7430)
                     /locus_tag="ISB30_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP1989971.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPHSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQCARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQCVARY
                     AMILLMLAEFGAWIWLR"
     gene            complement(7431..8009)
                     /locus_tag="ISB30_002343"
     CDS             complement(7431..8009)
                     /locus_tag="ISB30_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP1989972.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPIIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8032..8463)
                     /locus_tag="ISB30_002344"
     CDS             complement(8032..8463)
                     /locus_tag="ISB30_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP1989973.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQLCALSGYDELHISPVK"
     gene            8835..9854
                     /gene="epd"
                     /locus_tag="ISB30_002345"
     CDS             8835..9854
                     /gene="epd"
                     /locus_tag="ISB30_002345"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAP1989974.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9904..11067
                     /gene="pgk"
                     /locus_tag="ISB30_002346"
     CDS             9904..11067
                     /gene="pgk"
                     /locus_tag="ISB30_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP1989975.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11281..12360
                     /gene="fbaA"
                     /locus_tag="ISB30_002347"
     CDS             11281..12360
                     /gene="fbaA"
                     /locus_tag="ISB30_002347"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAP1989976.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12551..13411
                     /locus_tag="ISB30_002348"
     CDS             12551..13411
                     /locus_tag="ISB30_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAP1989977.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13550..14185
                     /gene="argO"
                     /locus_tag="ISB30_002349"
     CDS             13550..14185
                     /gene="argO"
                     /locus_tag="ISB30_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAP1989978.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14278..15018
                     /locus_tag="ISB30_002350"
     CDS             14278..15018
                     /locus_tag="ISB30_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAP1989979.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15186..15764
                     /locus_tag="ISB30_002351"
                     /pseudo
     CDS             15186..15764
                     /locus_tag="ISB30_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15771..16082
                     /locus_tag="ISB30_002352"
     CDS             15771..16082
                     /locus_tag="ISB30_002352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989980.1"
                     /translation="MRFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGH
                     FLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTN
                     H"
     gene            complement(16079..17134)
                     /locus_tag="ISB30_002353"
     CDS             complement(16079..17134)
                     /locus_tag="ISB30_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAP1989981.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLATHRGYD
                     SDNPRVAGDVGKAGVAIDTVEDIKVLFDQIPLDKMSVSMTMNGAVLPVLAFYIVAAEE
                     QGVTSDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISG
                     YHMGEAGANCVQQVAFTLADRIEYIKAAISAGLKIDDFAPRLSFFFGIGMDLFMNVAM
                     LRAARYLWSEAVSGFGAQDPYNNVIRTTIDHCAHPMYRDYLHRYLENASGGHIHHDLS
                     HVFDLHRNLIATGSMLG"
     gene            complement(17337..18230)
                     /locus_tag="ISB30_002354"
     CDS             complement(17337..18230)
                     /locus_tag="ISB30_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAP1989982.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            18371..18601
                     /locus_tag="ISB30_002355"
     CDS             18371..18601
                     /locus_tag="ISB30_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1989983.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            18657..19316
                     /gene="rpiA"
                     /locus_tag="ISB30_002356"
     CDS             18657..19316
                     /gene="rpiA"
                     /locus_tag="ISB30_002356"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP1989984.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            19572..20804
                     /locus_tag="ISB30_002357"
     CDS             19572..20804
                     /locus_tag="ISB30_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP1989985.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            21033..21092
                     /gene="ibsC"
                     /locus_tag="ISB30_002358"
     CDS             21033..21092
                     /gene="ibsC"
                     /locus_tag="ISB30_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAP1989986.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(21193..21795)
                     /locus_tag="ISB30_002359"
     CDS             complement(21193..21795)
                     /locus_tag="ISB30_002359"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP1989987.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNKLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(21816..21999)
                     /gene="ssrS"
                     /locus_tag="ISB30_002360"
     ncRNA           complement(21816..21999)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ISB30_002360"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22041..22370)
                     /gene="zapA"
                     /locus_tag="ISB30_002361"
     CDS             complement(22041..22370)
                     /gene="zapA"
                     /locus_tag="ISB30_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP1989988.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            22538..23116
                     /gene="ygfB"
                     /locus_tag="ISB30_002362"
     CDS             22538..23116
                     /gene="ygfB"
                     /locus_tag="ISB30_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAP1989989.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23142..24467
                     /gene="pepP"
                     /locus_tag="ISB30_002363"
     CDS             23142..24467
                     /gene="pepP"
                     /locus_tag="ISB30_002363"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAP1989990.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            24464..25642
                     /gene="ubiH"
                     /locus_tag="ISB30_002364"
                     /gene_synonym="visB"
     CDS             24464..25642
                     /gene="ubiH"
                     /locus_tag="ISB30_002364"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAP1989991.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQYGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERREDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            25665..26867
                     /gene="ubiI"
                     /locus_tag="ISB30_002365"
                     /gene_synonym="visC"
     CDS             25665..26867
                     /gene="ubiI"
                     /locus_tag="ISB30_002365"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAP1989992.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGIGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRACCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKALQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
BASE COUNT         6521 a         6558 c         7279 g         6596 t
ORIGIN      
        1 tgcacgagta ttttattttt accgccggac ggtaccctaa aaataaaata aggataattt
       61 atgcggctta ttgattattt tccagaatca tcaatctcag ttatacattc agcaaaagac
      121 tggcaggaag ctatcgattt ctcgatggca tcattgctgg ataaaaacta tatcagcgag
      181 aattacattc aatctattaa agactccacc atcagcaatg gtccttatta tattctcgca
      241 ccaggcgtgg caatgcctca tgcgcgaccg gaatgtgggg cgcttaaaac cgggatgtct
      301 ttgacgttac ttaaacaagg cgtttatttc ccggggaagg acgaaccgat taaattactc
      361 atcgggcttt ctgctgccga tgccaattcg cacattggcg ctattcaggc gttaagtgaa
      421 ttactgtgcg aagaagaaat actcgaacaa ctcttaacag catcatcaga aaaacaatta
      481 gcggacatta tcagccgcgg ataattactc tctcctctac aggataatat tatggaaaac
      541 aagtctgctc gtgcaaaggt ccaggctttt gggggctttt tgactgcaat ggtcatcccc
      601 aatatcggtg cttttattgc ctggggattt ataacggcat tatttattcc caccggctgg
      661 ctgccgaatg aacatttcgc caaaattgtt ggtccgatga ttacctattt attgcccgtg
      721 atgattggtt ctaccggtgg tcatctggtc ggcggtaaac gcggcgcggt catgggcgga
      781 attggtacga ttggtgtgat cgtcggcgct gaaatcccga tgttccttgg ctcgatgatt
      841 atggggccgc tcggtggtct ggtgattaaa tatatcgaca agtcgctgga aaaacgcata
      901 cctgctggtt ttgagatggt tatcaataac ttctcattag gtatcgcggg gatgctcttt
      961 tgtctgctgg gttttgaagt tatcggtccg gcggtgttaa ttgctaatac tttcgtcaaa
     1021 gagtgtattg aggcgctggt acatgcgggt tatctgcctt tgctgtcagt catcaatgaa
     1081 ccggcgaaag tgcttttcct taataatgcg attgatcagg gcgtctatta tccgctggga
     1141 atgcaacagg cttcggttaa cggtaaatcc atcttcttca tggtggcctc caacccaggt
     1201 cctggtctgg ggctgctgct ggcgtttacc ttgtttggta aagggatgag taaacgttct
     1261 gcgcccgggg cgatgattat tcacttcctc ggtgggatcc acgaactgta tttcccgtat
     1321 gtgctgatga agccgctgac cattattgcc atgattgcgg gcggtatgtc tggcacctgg
     1381 atgtttaact tactggacgg tggtctggtg gctggcccaa gtccggggtc tatctttgct
     1441 tacctggcac tgacgccgaa aggctcgttc ctggcgacaa ttgccggtgt tacggtaggt
     1501 accctggtgt cctttgctat cacttcgctg atactgaaga tggaaaaaac ggtggaaacg
     1561 aagagcgaag atgagtttac cgagtcagcc aatgcggtta aggcgatgaa acaagaaggt
     1621 gcattctcgt taagccgtgt taagcgtatt gcctttgttt gcgatgcagg gatgggctcc
     1681 agtgcgatgg gcgcaaccac cttccgtaaa cgcctggaaa aagcggggct ggcaatagaa
     1741 gtaaaacatt acgccataga aaacgtgcct gcggatgcgg atatcgtcgt tactcacgcc
     1801 agtctggaag ggcgcgtgaa acgtgtgacg gataaaccac tgatattgat taataactat
     1861 attggcgatc caaaactcga cactttattt aatcaattaa ccgctgaaca taaacactga
     1921 ttacagaggt aaaaatgaaa accaaagttg ctgctattta tggcaagcgg gatgtccgtc
     1981 tgcgcgaatt tgaactgcca gaaattactg ataatgaact gttagtgagt gtaatttctg
     2041 acagcgtttg tttatccacc tggaaagcgg cgttgcttgg tagtgaacat aaacgcgtcc
     2101 ccgacgattt agaaaatcat ccggttatta ccgggcatga atgtgctggg gttgttgtcg
     2161 aagtgggtaa aaatctcact ggcaaatata aaaaagggca gcgttttgta ttgcaaccgg
     2221 cgatggggct accaagcgga tattcagccg gatacagcta cgaatatttt ggtggcaatg
     2281 ccacctatat gattattccc gaaatcgcga ttaatttagg ctgtgtatta ccgtatcacg
     2341 gctcttattt tgctgcggcg tcgctggcag agcctatgtg ctgcattatt ggtgcttatc
     2401 atgccaatta tcacaccacg caatatgttt atgagcatcg tatgggcgtc aaacctggcg
     2461 gcaatattgc cctgctggcg tgtgctggcc cgatgggcat tggcgctatc gattacgcca
     2521 ttaacggcgg catacaaccg gcgcgggtgg tggtagtcga tatcgacgac aaacggctgg
     2581 cgcaggtgca gaagttgctg ccggtggagc tggcggccag taaaggcatt gagctggtgt
     2641 atatgaatac caaagggatg agcgatccgg tccagacgct gcgggcgctg acggatgatg
     2701 ccggatttga tgacgtattt gtctatgcag cggtgccttc agtcgtcgag atggctgatg
     2761 aattactggc tgaagatggc tgcctgaact tctttgccgg tccgacggat aaaaacttca
     2821 aagtgccgtt taatttctac aacgtccatt acaacagtac gcacattgtc ggcacgtctg
     2881 gcggttccac ggacgacatg aaagaggcga ttgcccttag cgccacgggg cagttacagc
     2941 cgtcgtttat ggtgacccat attggtggcc tggatgcggt gccagaaacc gtgctcaatc
     3001 tgccggatat ccctggcggt aaaaaactca tttataacgg cgtgaccatg ccgctgaccg
     3061 ccattgccga ttttgccgaa aaaggcaaaa ccgatccgct gtttaaagag ctggcgcggc
     3121 tggttgagaa aacgcacggt atctggaatg aacaggccga gaaatatctg ctggcacaat
     3181 ttggcgttga tattggggag gccgcgcaat gatgtccctg gcgtggccat tatttcgcgt
     3241 tacggaacag gcagcgttgg ctgcctggcc gcaaaccggc tgtggcgaca aaaataaaat
     3301 tgatggcctt gcggtcaccg cgatgcgcca ggcattaaac gacgttgctt ttcgtgggcg
     3361 agtggttatc ggcgaggggg aaattgacca tgcgccgatg ctgtggattg gcgaagaggt
     3421 gggcaaaggc gatgggccag aagtcgatat cgcggtcgat cccattgaag gcacgcggat
     3481 ggtggcgatg gggcagagca atgcgctggc ggtcatggct ttcgcccctc gagatagcct
     3541 gttacatgcg cccgatatgt atatgaaaaa gctggttgtt aatcgactgg ctgccggggc
     3601 gatagatctg tctttgcctc tggcggacaa cttgcgtaac gtggcgcggg cgttaggtaa
     3661 accgctggac aagctgcgca tggttacgct ggataaaccg cgcctgagtg ccgcaattga
     3721 ggaagcaaca cagctcggcg tgaaggtttt tgccctgccg gatggcgatg tcgccgccag
     3781 cgtgctgact tgctggcagg ataatcctta cgatgtgatg tacaccatcg gtggcgcacc
     3841 ggaaggtgtt atttctgcct gtgccgttaa agcattaggc ggggatatgc aggccgagct
     3901 gattgacttt tgtcaggcta aaggggatta cacggaaaat cggcagattg cagagcagga
     3961 gcgcaagcgt tgcaaggcaa tgggcgttga cgttaaccgt gtatactcgc tcgatgaact
     4021 ggtgaggggg aacgatatcc tctttagcgc tacgggcgtg acgggcggcg agttggtgaa
     4081 cggtatccaa cagacggcga atggggtgcg gacgcagaca ttactgatcg gcggcgcgga
     4141 ccaaacgtgt aatataatag actccctgca ttgatggtga tctatcggaa gaccaatggc
     4201 gacgctgaca gaagatgatg tgcttgagca actgaatgca caggacaatt tattttcatt
     4261 tatgaaaact gcgcattcta ttttgctcca ggggatacgc cagtttctgc cgtcgctgtt
     4321 tgtcgataac gatgaagaga tcgtcgaata tgcagtgaag ccgttactcg cccaaagcgg
     4381 cccgcttgac gatattgatg ttgcgctgcg tttgatttat gcgctgggga aaatggataa
     4441 atggctgtac gccgatatca cgcatttttc ccagttttgg cattacctga acgaacagga
     4501 tgaaacgccc agatttgctg atgacataac ctgggatttt atcagcaacg tcaatagcat
     4561 aacccgcaat gcgacgctct atgacgcgtt gaaagcgatg aagttcgccg atttcgccgt
     4621 ctggtcagag gcgcgtttta gcgggatggt caaaacggcg ctgacgctgg cagtaacgac
     4681 caccttaaag gaattaacgc cgtgaaaatc gaactgacgg tgaatggact aaaggtacag
     4741 gcgcagtact ctgatgatga aattgaaaat gtacataaac cattgctgcg tatgctggcg
     4801 gccttacaga cagtaaatcc gcagcggcgt acggtggttt ttctttgcgc tccgccgggg
     4861 acgggtaaat ctaccctaac caccttctgg gaatatctcg cgcaacaaga cccggaactg
     4921 cccgctattc agacgctgcc gatggatggt tttcaccatt acaatagctg gctggatgtg
     4981 catcaattgc gacccttcaa aggcgcacca gagacattca acgttgcgaa actggcggaa
     5041 aatctgtgcc gggttgtgga aggggattgt acgtggccgc agtacgatcg acaaaagcat
     5101 gatcctgttg aagatgtgtt gcacgttacc gcaccactcg tcatcgtcga aggaaactgg
     5161 ttgttgttgg acgacgagaa gtggtgccag cttgcgcaat tttgtgattt ctcgatcttt
     5221 atcaaagctc ccgcatctgc gttgcgggag cgtctggtgg ggcgcaagct ggcgggaggg
     5281 ttatcgctgg cagacgcaga agccttttat gaccgtaccg acgggccgaa tgttcgccgg
     5341 gtgctggaag agagtctgcc tgcaaatttg accttaatga tgaccgctac gggtgaatat
     5401 cgtctgatgg attaacgttc ctgattacac catcttccag tcgcaccaca cgagagaaat
     5461 gacgccgggt aaatgcggcg tcatgactga ttgctacgac gctcgtgcct cgctggcggc
     5521 acttttccag ccagctttca aacacgctca accaattttc gtcaaaatcc cgactcggct
     5581 cgtccagtag caatagtggc ggagagagtg cttccaggca ggcgacggcg accatccgtc
     5641 gctgcgcact atgcagatct aacggatgcg cgctggcagt gtctgctaac tgacagcatt
     5701 gcagggcagc attggtacgt tgagtgattt catccgccgg gcatttttgc agtatcaaac
     5761 caaatgcgat ttcgtcagca acggtgctgt gaaaaagctg attttccgct tcctgaaaca
     5821 gtacgccaat ctttgccgcg cgctggcggt ttttcaggtt tttcatcgct tgttgttgca
     5881 gcataaccgt accggctgtg ggcgtaagaa gacctgccat tactcgtaac agcgtcgatt
     5941 ttcccgcgcc attgtcgccg gtcagcgcca gccattcacc ttgtttgagt tgcagagaaa
     6001 tgtcgcaaag acaatccgta gccgcgccag gccagcgata agatatctga tttaacgtta
     6061 acatagtggc tggagcgctc cgttttgcaa ttgccacgct tgctggcagt ggttcaggaa
     6121 aggtgtgtga tgacgttcaa aaagaataat cagcgaacca cgttcaagcg cccagtgttg
     6181 taagcgctgc aacagcattt cgcgggcctg cggcgtcagg cggctaaagg cttcatcaag
     6241 aattaagagc tttggctgca tcgcgatggc gcaggcgatg accacacgct gtgtctctcc
     6301 accggagagt gttgccgggt gacgatggcg caacggctgg cactcggtca gggccagggc
     6361 ggcgtcaatc cgcgccataa tctctttttc agcaagacac agattttctg gcccaaaggc
     6421 aacttcttct tccacgctaa aggtacagcc ggagagctgc aaatagggcg attgctggac
     6481 gagctggatg gtggcggatt gttcattaag cgggagacgc ccaatgggcg taccgagaag
     6541 cgtccccgtg ccggttatct ctcctggtag aaagtccgga taccagcccg ccatcagctg
     6601 tgccagcgta cttttgccac tgccgttgtc accaaagatg gcgaccatcc ccggtttgac
     6661 atagtgaaag tcataacaga acggaggatg cgttgagtgt gtcgggcaat agcgaaactg
     6721 ttctaacgta accatatcca cgctccgaat tcagccagca ttagcaatat catggcgtaa
     6781 cgtgctacgc attgtagggt gctatcggct ggtgcccata acgtagtgcg cctattgttg
     6841 attcgaaatg cccgcatatc cagcgccgca ccacggacag caagatcgtt tagcgcattg
     6901 tgtgttagcg gaataattaa cgcgggcatc gcacgtaaac gctgatacca gccctcatcc
     6961 agcggcacgc cgcgggcgca ctgcgcttca tgaataatcg ccaattgccg ttttaactgc
     7021 tcaacgacca ataacggccc ggcaaacaga taagcgacgc ccggcggcag gcgagaagca
     7081 aacaaagcgc ggataaaacg ttgcacgggg acgtactgca tccacaactg tgaggttgaa
     7141 acaatcgcca gaatccgcaa ccagagcgta atagcatgcg cccaccgttc tggagagtgt
     7201 ggcgtgccgc ttagccattc tgtcagccag ccaccgtgca ccagccacag tccagccccc
     7261 agcgagaaca tcagccagac gacatatttt gcccgccgcc gcgtggcctt tagcgcaata
     7321 aggcagaaaa aggtcgccgc gctatagact ggcaatattg tctgtgtagg caggataagc
     7381 gtggtgcagg ccgccagcgc ccacagcgtt aaggatgtaa aaggatgcat ttagcgcacg
     7441 gcagccattg ccggaaaatg gcgcgtggtg cgaatcggca gctgacgcag taaaagccag
     7501 actatcaccg ctgtaagaat tttatcgacc aggttcgcgc ctatcaccgt aatcgccacc
     7561 gactccacca ggttttgccc catcgagtgc atccaggcaa cgaacaaatc ggctccgctt
     7621 cctgtcacgc cgccgaacag cgcagtacgc agcggtacag cgacgaccgt taccgcaagc
     7681 gtaataatca cgccgctaac aacgactttt ggcagggtgc gaaaccaacc cgctcgcgcc
     7741 agccaaccgg cgaccaggcc gatgaccatc gcgacgggcg caaatgcggc ggcgatcggg
     7801 tcggtcagca atccccagag taaattggtc agtaaaccgg tcagcatccc gattatcgga
     7861 ccaagcagta ccgcgctaat aagtgtgccg atggagtcga gaaaaatagg taatttaacc
     7921 atgctggcca gttgcccgcc gatcatattg atggcgatag agataacgat caacaccagt
     7981 gcctgactgg aaaagtggcg acgcgccata agaaaacctc tctgaaaata gttatttaac
     8041 gggggaaata tgcaactcgt cgtaaccaga aagcgcgcaa agctggcggc taaacgccag
     8101 ttcctcaagg cagccgatca ccagttcaag cgtggtccgc acggtacagc cgggcatcat
     8161 atgaccgcca agcatcgtgc cgtgtggatc ggaaacgcag agatgaagat gttcaccact
     8221 ctgttccagc gtgccgttca atgcgatgac ttcgaactta ccgcttagca gggcagtgtt
     8281 ttcttgcccg gcatagcgta aggcgacgtc agtcagactg ccggtacaac ctgcaatcca
     8341 cgcggcgtga agttgttgtt gctgcgcgaa ggcgcgtaat tgggagagaa cttcctgccc
     8401 ggggagcagg cgcagcgcgt aaaagcgtgc ggaggaagaa tatgggtagg gcaatgtcat
     8461 cattggagta ctccgaaact cggacgtatt gcggataata tcgacgctgc atcagtcata
     8521 ttctgatata agtcagggtt tgtgaccgca caacgaaaag atattgctac gcttattcgt
     8581 gattctgcaa agtggaataa attccacagg ggcggggatg aaaacgtgac ataagtcaga
     8641 taattgtctt ctttgactgg acaaacattc cttttattcc acgtttcgct tatcctagct
     8701 gaagcgtttc agtcgattaa atgttcgaca attaaccaat cagtcgcagt ttgcgacagg
     8761 taaggtttcc ccgaacgatt tgctggatta ctctgtctgg caaatttgct cagggaaaac
     8821 cttgcaggag atctatgacc gtacgcgtag cgataaatgg cttcggtcgc atcgggcgta
     8881 atgtggttcg tgctttgtat gaatccggac gccgtgcgga aattaccgtg gtggcaatca
     8941 acgaactggc ggatgctgcg ggcatggcgc atttgttgaa atatgacacc agccatggcc
     9001 gttttgcatg ggaagtacga caggaacgcg atcaactttt tgttggtgat gacgccatcc
     9061 gtgtattgca tgaacgttca ctgcaatcgc tcccctggcg tgaacttggc gttgatgtag
     9121 tcctcgactg caccggcgta tatggctccc gcgagcatgg tgaagcacac attgccgccg
     9181 gggctaaaaa agtgctcttt tcacatcctg gcagtaacga tctcgacgcg accgttgttt
     9241 atggcgtcaa tcaggatcaa cttcgtgcgg aacaccgcat cgtttctaat gcttcctgta
     9301 ccacgaattg cataattccc gtcatcaaat tgttagatga tgcgtacggt attgagtctg
     9361 gcactgtgac cacaattcac tccgccatgc atgatcaaca ggttattgat gcataccatc
     9421 ctgacctgcg tcgcactcgg gcagccagtc agtcgatcat tccggtcgat actaaactgg
     9481 ccgccggtat cacacgattt tttccgcaat ttaacgatcg ctttgaggcg attgcggtac
     9541 gtgtgccaac cataaatgtg acggcaatcg atttaagcgt gacggtgaag aaacctgtaa
     9601 aagccaatga agtcaacctg ttgctgcaaa aagcagcaca aggtgcattt catggtatag
     9661 ttgactatac ggaattgccg ttggtctctg tagattttaa ccacgatccg cacagtgcca
     9721 ttgtcgatgg cactcaaacc cgggtcagtg gcgcacacct gatcaaaacg ttggtctggt
     9781 gcgataacga atggggcttt gctaaccgaa tgctcgacac gacgttagct atggctactg
     9841 ttgctttcag gtaagacgca agcagcgtct acaaaacttt tagaatcaac gagaggattc
     9901 accatgtctg taattaagat gaccgatctg gatcttgctg ggaaacgtgt atttatccgt
     9961 gcggatctga acgtaccagt aaaagacggg aaagtaacca gcgacgcgcg tatccgtgct
    10021 tctctgccga ccatcgaact ggccctgaaa caaggcgcaa aagtgatggt aacttcccac
    10081 ctgggtcgtc ctaccgaagg cgagtacaac gaagaattct ctctgctgcc ggttgttaac
    10141 tacctgaaag acaaactgtc taacccggtt cgtctggtta aagattacct cgacggcgtt
    10201 gacgttgctg aaggtgaact ggttgttctg gaaaacgttc gcttcaacaa aggcgagaag
    10261 aaagacgacg aaactctgtc caaaaaatac gctgcactgt gtgacgtgtt cgtaatggac
    10321 gcattcggta ctgctcaccg cgcgcaggct tctactcacg gtatcggtaa attcgctgac
    10381 gttgcgtgcg caggcccgct gctggcagct gaactggacg cgctgggtaa agcactgaaa
    10441 gaacctgctc gcccgatggt ggctatcgtt ggtggttcta aagtatctac caaactgacc
    10501 gttctggact ccctgtctaa aatcgctgac cagctgatcg ttggtggtgg tattgctaac
    10561 acctttattg cggcacaagg ccacgatgtg ggtaaatccc tgtacgaagc tgacctggtt
    10621 gacgaagcta aacgtctgct gaccacctgc aacatcccgg ttccgtctga tgttcgcgta
    10681 gcaaccgagt tctctgaaac tgcaccggct accctgaaat ctgttaacga tgtgaaagct
    10741 gacgagcaga tcctggatat cggtgatgct tccgctcagg aactggctga aatcctgaag
    10801 aatgcgaaaa ccattctgtg gaacggtccg gttggcgtgt tcgaattccc gaacttccgc
    10861 aaaggtactg aaatcgtggc taacgctatc gcagacagcg aagcgttctc catcgctggc
    10921 ggcggcgaca ctctggcagc aatcgacctg ttcggcattg ctgacaaaat ctcctacatc
    10981 tccactggcg gcggcgcatt cctcgaattc gtggaaggta aagtactgcc tgcagtagcg
    11041 atgctcgaag agcgcgctaa gaagtaaaaa atcacagggc agggaaacct gcccttgttt
    11101 cagcgcgctt ttagagcacg cactttttca tactctaaat aattcgagtt gcaggaaggc
    11161 gacaagagag tgaatcccag gagcttacat aagtaagtga ttggggtgag cgaacgcaga
    11221 cgcagcacat gcaacttgaa gtatgacgag tataaggccc gacgatacag gacaagagac
    11281 atgtctaaga tttttgattt cgtaaaacct ggcgtaatca ctggtgatga cgtacagaaa
    11341 gttttccagg tagcaaaaga aaacaacttc gcactgccag cagtaaactg cgtcggtact
    11401 gactccatca acgccgtgct ggaaaccgct gctaaagtta aagcgccggt tatcgttcag
    11461 ttctccaacg gtggtgcttc ctttatcgct ggtaaaggcg tgaaatctga cgttccgcag
    11521 ggtgctgcta tcctgggcgc gatctctggt gcgcatcacg ttcaccagat ggctgaacat
    11581 tatggtgttc cggttatcct gcacaccgac cactgcgcga agaaactgct gccgtggatc
    11641 gacggtctgc tggacgcggg tgaaaaacac ttcgcagcta ccggcaagcc gctgttctct
    11701 tctcacatga tcgacctgtc tgaagaatct ctgcaagaga acatcgaaat ctgctctaaa
    11761 tacctggagc gcatgtccaa aatcggcatg actctggaaa tcgaactggg ttgcaccggt
    11821 ggtgaagaag acggcgtgga caacagccac atggacgctt ctgcactgta cacccagccg
    11881 gaagacgttg attatgcgta taccgagctg agcaaaatca gcccgcgttt caccatcgca
    11941 gcgtccttcg gtaacgtaca cggtgtttac aagccgggta acgttgttct gactccgacc
    12001 atcctgcgcg actctcagga atatgtttct aagaaacaca acctgccgca caacagcctg
    12061 aacttcgtat tccacggtgg ttccggttct actgctcagg aaatcaaaga ctccgtaagc
    12121 tacggcgtag tgaaaatgaa catcgatacc gatacccaat gggcaacctg ggaaggcgtt
    12181 ctgaactact acaaagcgaa cgaagcttac ctgcagggtc agctgggtaa cccgaaaggc
    12241 gaagatcagc cgaacaagaa atactacgat ccgcgcgtat ggctgcgtgc cggtcagact
    12301 tcgatgatcg ctcgtctgga aaaagcattc caggaactga acgcgatcga cgttctgtaa
    12361 gtcattcttt tctgcttatc tcaaggcccg ctctgcgggt ctttttttcg ccaaaagcaa
    12421 ggtgatgcag atgagaaatc tgtgatctat ttggcaaaat tatgctttat tgtttaccct
    12481 tgtcagactg cccgccataa ggcggcggag tgtatttctc cattttgagt cagttgaaaa
    12541 ggaatattga atggaagatt tgaatgttgt cgatagcata aacggcgcgg gaagctggct
    12601 ggtggctaac caggcgctgc tgctaagtta tgcagtaaac atcgtggcgg cactcgcgat
    12661 catcatcgtt ggtttgatta tcgcgcggat gatttccaac gcggtgaatc gcctgatgat
    12721 ctcccgtaaa atcgatgcca ctgttgctga ttttctttct gcattagtcc gttacggaat
    12781 tatcgccttt acgctaatcg ctgcactggg gcgcgtgggc gtacaaacag cgtcagtcat
    12841 tgctgtactc ggtgccgcag gcttagctgt tggtctggct ttgcaggggt cactgtctaa
    12901 cctggccgct ggcgtgttac ttgtcatgtt ccgcccgttc cgtgccggtg aatatgttga
    12961 cctcggcggc gtagccggca ctgtgctgag tgtgcagatt ttctccacca ccatgcgtac
    13021 tgcagacggt aaaattatcg ttattccgaa tggtaaaatt attgccggga atattattaa
    13081 cttctcccgc gagccagctc gccgtaacga atttattatt ggcgtggcgt atgattccga
    13141 tatcgatcag gttaagcaga tcctgaccga tattatccag tctgaagatc gcattttgaa
    13201 agatcgcgaa atgactgtgc gtctgaacga acttggtgca tcgtcgatta atttcgtggt
    13261 ccgagtctgg agcaacagcg gcgatctgca aaacgtgtac tgggatgtgc tggagcgtat
    13321 taaacgtgaa tttgatgccg ccggtatcag cttcccgtat ccgcaaatgg atgtgaactt
    13381 taagcgggtg aaagaagaca aagctgcgta atcaacgctg aggccagata atactctctg
    13441 gcctctctct tattagtttt tctgattgcc aattaatatt atcaatttcc gctaataaca
    13501 atcccgcgat atagtctctg catcagatac ttaattcgga atatctaacg tgttttctta
    13561 ttactttcaa ggtcttgcac ttggggcggc tatgatccta ccgctcggtc cacaaaatgc
    13621 ttttgtgatg aatcagggca ttcgtcgtca gtaccacatt atgattgcct tactttgtgc
    13681 tatcagcgat ttggtcctga tttgtgcggg gatttttggt ggcagcgcgt tattgatgca
    13741 gtcgccgtgg ttgctggcgc tggtcacctg gggcggcgta gccttcttgc tgtggtatgg
    13801 ttttggcgct tttaaaacag caatgagcag taacattgag ttagccagtg ccgaagtcct
    13861 gaaacaaggc agatggaaaa ttatcgccac catgttggca gtgacctggc tgaatccgca
    13921 tgtttacctg gatacttttg ttgtgctggg cagccttggc gggcaacttg atgtggaacc
    13981 aaaacgttgg tttgcgctcg ggacaattag cgcctctttc ctgtggttct ttggtctggc
    14041 tattctcgca gcctggctgg caccgcgcct gcgcacggca aaatcacagc gcattatcaa
    14101 tctggtagtg ggatgtgtta tgtggtttat tgccttgcag ctggcgagag acggtattgc
    14161 tcatgcacaa gccttgttca gttaggcgct atctgatgga aaaataaaac agaggcgcta
    14221 agcttgcctc cagaggtcct gaattttggg caagtcaatt aacacggaga gactaacgtg
    14281 aagttcaaag ttatcgccct ggcggcatta atgggtatta gcgggatggc agcgcaggct
    14341 aacgaattgc cggatggacc gcatattgtc acctccggta cggcaagcgt ggatgcggtg
    14401 ccagacattg ccactcttgc gattgaagtt aacgtggccg cgaaggatgc cgctactgcc
    14461 aagaaacagg cagatgagcg cgtcgcacaa tacatttcct tccttgagct caatcagatc
    14521 gcgaaaaaag atatcagctc agcgaactta cgcactcagc cagattatga ttatcaggat
    14581 ggtaaaagta tcctgaaagg ctaccgcgca gtgagaacgg tggaagtcac gctccgtcag
    14641 ttagacaaac tgaattcctt gctggacggc gcgctaaagg cgggtcttaa cgaaattcgt
    14701 tctgtgtcgc tgggcgtggc gcagccggat gcctataaag acaaagcgcg taaggcagcg
    14761 attgataacg cgattcatca ggcacaggaa ctggcgaatg gctttaatcg taaactgggg
    14821 ccggtatata gcgtgcgcta ccatgtttcc aactatcagc ccagcccaat ggtgcggatg
    14881 atgaaagctg atgctgcacc ggtgtccgca caggaaactt acgagcaggc cgctattcag
    14941 tttgatgatc aggtcgatgt agtcttccag ttagaacctg tggatcaaca acctgctaaa
    15001 acacctgcag cacaataata cttgtagccc gacgtattcg tcgggctaat gcctttaccc
    15061 gatattgcct cctttttctg taattttctg taaaaccatg acgtaatcac ctatctttgc
    15121 tcgcggtttc atcaaggata ggctgactga aacggttaag cggacaagga tgcgggagag
    15181 gggggatgga catttttatt tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca
    15241 ataatattgc ccgggcggca gaaaaaatcc atatgacggc ttcgccattt ggcaaaagca
    15301 ttgccgcgct ggaagaacaa attggctata cgctatttac ccgcaaagat aataatatca
    15361 gcctcaataa agccggacag gaactgtatc aaaaactgtt cccggtttat caacgacttt
    15421 ctgcgattga taatgaaatc cataactcag gacgtcgtta acgagaaatt gtgataggca
    15481 tagataatac ttatccgaca attatttttg atcagttgat tagtctcgga gataagtacg
    15541 aaggcgtgac catccagcca gttgagttca gcgaaaatgg cgttatcgat aatctttttg
    15601 accgtcagtt agattttatt atctccccac agcatgtatc tgcacgagtt caggaactgg
    15661 aaaacctgac tatcagtgaa ctaccaccgc tgcgattggg ctttctcgtt tctcgccgtt
    15721 atgaagggcg acaggagcaa gagctgcttc aggagctgcc ctagctacaa atgcgctttc
    15781 aaaaccgggc taacttcgaa gcaatgattg atgcgaatat gcgtccttgt gggatcaatc
    15841 caaccattat ttatcgtccc tatagtttta tggctaaaat cagcgccgtg gagcggggac
    15901 attttctgac ggtgatcccg cattttgcct ggcgactggt gaatccggca acgctgaaat
    15961 attttgatgc gccgcacagg ccgatgtata tgcaggaata tctttattca atcagaaatc
    16021 atcgttatac cgccacgatg cttcagcata ttgctgaaga tcgtgacggg acaaaccatt
    16081 aacccagcat cgatccggtt gcaattaaat tacggtgtaa gtcgaagacg tggctaagat
    16141 cgtggtgaat atgtccgccg gacgcatttt ccagatatcg atgcagataa tcccgataca
    16201 tgggatgtgc acaatgatca atggtggtgc ggataacgtt gttatacgga tcctgtgcgc
    16261 caaatccact gaccgcttcg ctccataaat aacgtgccgc acgcaacatg gcgacgttca
    16321 taaacagatc catgccaatg ccgaagaaaa acgacaggcg aggggcgaag tcatcaattt
    16381 tcagcccggc ggagattgct gctttgatgt actcaatcct atcggcgagc gtaaatgcta
    16441 cctgctgcac gcagttggca cccgcttcac ccatgtggta accgctgata ctgatggtat
    16501 taaatcgcgg catgttgccg gaacaccagg cgatgatgtc tgcgataata cgcattgacg
    16561 gttttggtgg gtaaatatag gtgttgcggc aaagatactc tttgaggata tcgttttgaa
    16621 tggtgccggt cagtttatcg gatgtaacac cttgctcttc tgcggcgacg atataaaacg
    16681 ccagtactgg tagcactgcg ccattcatgg tcatcgaaac cgacatttta tccagcggga
    16741 tctggtcgaa caggactttt atatcttcca cggtgtcgat agcgacgccc gctttgccga
    16801 cgtcgcccgc cacgcgtggg ttatcggagt cgtagcctcg gtgggtggcc aggttacggc
    16861 gataaaaagc gttggactct tttgctgtcg agaaaccagc atactgacgg atggtccacg
    16921 gttgggcggt atacatagtg gcacgcgggc cacgaacgta ggggggcaaa ccaggaaggg
    16981 tacccgtcac ctccagatta tcgagatcgg cttcggtata cagcggcttg atggcgatcc
    17041 cttccgcggt ttgctgaacc agcgagtcga cagttttctc ccgacggctc aattccttgt
    17101 tggcaagctg ttgccactcc tgcacgttag acattatttg ctccgtaaaa cgcaatcaaa
    17161 atgagggtat ttgcgcttac ggtgagcttt ttacgtgggt ttgtcgccag caaaaaaatg
    17221 cattcagacg tcgttttttg ccgcctgaga tttgtctatt ttgcgatgtg attcaaggaa
    17281 tacaaaaaga agtgaggata acgcctgata tgtgctatca ggcgttattt gatggattaa
    17341 tcctgacgaa gaactttgtg accataatcg agcaacgcat cagtgacttt acgcatcatg
    17401 cggctttcag gagcaaagcg gtgccagtag agcatccggc gttgaaatag ccctggcgtt
    17461 aagtcaatca gttcgccgct ggccagctct ttctcgattt gcaggtgcgg gatcatacag
    17521 caggtggtgc cctgacgagc aagttgcacg aacgcttccg aagaattaac gatatggcag
    17581 ggtacgctgc caggaggcag atcgaaattt tgctgcaaaa aggcctggtg catatcgtca
    17641 agatggtcaa acgcgaccac tggcgctttc agtaatgccg aacgcgttac gccgttaggg
    17701 aaatattttt cggcaaatgg ttttgagctg acgaacagat agtcgagcgc accgagttta
    17761 tcgacaagac aactcggcag cgcctgatgt tgaatactca ctgcgccgac cacttcgccg
    17821 cgacgcagac gttcctgagt gcgggtttca tcttctacct gcaagttgag gcggatgggc
    17881 gaatcagcca acacaggagc cagtgccgga agcaaccacg tcgccagact gtcggcgttg
    17941 accgccagtg aaagcagcag cggagtcgaa ccggtttgtt catcgcccag ccactcttct
    18001 tccagcagct ccacctggcg tagtagcgcc aacagttttt gcccttgttc cgtcgggcgc
    18061 ggcggtacgg tacgcactaa caacggctgc ccgaacatat tttccagttg cttaatgcgc
    18121 tgtgagacgg ctgattgtgt aatgcacagc ttttgtgccg cgcgctcaaa tcctcgttca
    18181 cgtatcaccg catccagtgc ctgtaatgtt ctgtagtccg ggcgtttcat tgctgttgtc
    18241 tgctcctggt aaatgctttt tcctgcacta tgacacaatt ttgtgtcggg ttgcatatac
    18301 cgaagccgtt ggcgggtaac atttgtatca gtggttaacg cgcagcttaa aagatcagag
    18361 atttgcgcta atgcatttcg ctcagcgagt tcgcgccctg gttgtgttga atggcgtggc
    18421 gttattgcct cagtttgcct gtaaacaggg gcttgcgaac ggggaactgg tgcgcctgtt
    18481 cgcaccgtgg agcggcatac ccagaccgtt gtatgcttta tttgcggggc gaaaagggat
    18541 gcccgccatt gcgcgatatt ttatggatga gttaaccacg cggcttgcca acggggtctg
    18601 aatcgctttt tttgtatata atgcgtgtga aatttcatac cacaggcgaa acgatcatga
    18661 cgcaggatga attgaaaaaa gcagtaggat gggcggcact tcagtatgtt cagcccggca
    18721 ccattgttgg tgtaggtaca ggttccaccg ccgcacactt tattgacgca ctcggtacaa
    18781 tgaaaggcca gattgaaggg gccgtttcca gttcagatgc ttccactgaa aaactgaaaa
    18841 gcctcggcat tcacgttttt gatctcaacg aagtcgacag ccttggcatc tacgttgatg
    18901 gcgcagatga aatcaacggc cacatgcaaa tgatcaaagg cggcggcgcg gcgctgaccc
    18961 gtgaaaaaat cattgcttcg gttgcggaaa aatttatctg tattgcagac gcttccaagc
    19021 aggttgatat tctgggcaaa ttcccgctgc cagtagaagt tatcccgatg gcacgtagtg
    19081 cagtggcgcg ccagctggtg aaactgggcg gtcgtccgga ataccgtcag ggcgtagtga
    19141 ccgataacgg caacgtgatc ctcgacgtcc acggcatgga aatccttgac ccgatagcga
    19201 tggaaaacgc cataaatgcg attcctggcg tggtgactgt tggtttgttt gctaaccgtg
    19261 gcgcggacgt tgcgctgatt ggcacacctg acggtgtcaa aactattgtg aaatgatctg
    19321 acgggggaac ctcccccgtt aaaaaaattc tcttcattaa atttggtgac atgtgtcacg
    19381 tttttaccag gcaattgtcg attgctctaa ataaatcctc taaaccagca tattcatcca
    19441 agaaatactt ttgcgtgata tttcctcaac atcgcgacgc aaacgttcat attgccgcaa
    19501 tattattttt tgatatgttg aaaggcggat gcaaatccgc acacaacatt tcaaaagaca
    19561 ggattgggta aatggcaaag gtatcgctgg agaaagacaa gattaagttt ctgctggtag
    19621 aaggcgtgca ccaaaaggcg ctggaaagcc ttcgtgcagc tggttacacc aacatcgaat
    19681 ttcacaaagg cgcgctggat gatgaacaat taaaagaatc catccgcgat gcccacttca
    19741 tcggcctgcg atcccgtacc catctgactg aagacgtgat caacgccgca gaaaaactgg
    19801 tcgctattgg ctgtttctgt atcggaacaa accaggttga tctggatgcg gcggcaaagc
    19861 gcgggatccc ggtatttaac gcaccgttct caaatacgcg ctctgttgcg gagctggtga
    19921 ttggcgaact gctgctgcta ttgcgcggcg tgccggaagc caatgctaaa gcgcatcgtg
    19981 gcgtgtggaa caaactggcg gcgggttctt ttgaagcgcg cggcaaaaag ctgggtatca
    20041 tcggctacgg tcatattggt acgcaattgg gcattctggc tgaatcgctg ggaatgtatg
    20101 tttactttta tgatattgaa aacaaactgc cgctgggcaa cgccactcag gtacagcatc
    20161 tttctgacct gctgaatatg agcgatgtgg tgagtctgca tgtaccagag aatccgtcaa
    20221 ccaaaaatat gatgggcgcg aaagagattt cgctaatgaa acccggctcg ctgctgatta
    20281 atgcttcgcg cggtactgtg gtggatattc cagcgctgtg tgacgcgctg gcgagcaaac
    20341 atctggcggg ggcggcaatc gacgtattcc cgacggaacc ggcgaccaat agcgatccat
    20401 ttacctctcc gctgtgtgaa ttcgacaatg tccttctgac gccacacatt ggcggttcga
    20461 ctcaggaagc gcaggagaat attggcctgg aagttgcggg taaattgatc aagtattctg
    20521 acaatggttc aacgctctct gcggtgaact tcccggaagt ctcgctgcca ctgcacggtg
    20581 gtcgtcgtct gatgcacatc cacgaaaacc gtccgggtgt gctgactgcg ctcaacaaaa
    20641 tttttgccga gcagggcgtc aacatcgccg cgcaatatct gcaaacttcc gcccagatgg
    20701 gttatgtagt tattgatatt gaagccgacg aagacgttgc cgaaaaagcg ctgcaggcaa
    20761 tgaaagctat tccgggtacc attcgcgccc gtctgctata ctaaatccct ttctctgaaa
    20821 atcaacgggc aggtcactga cttgcccgtt attttatccc ttctccacac cgttgccgcg
    20881 ttgcgcattc tactttgcga gtctgctcca gaaaataaat caaagtcaga tcaatgcgtt
    20941 gtgtttattt aatggggatt tacgatggcg ggactgtaca gggctgtaac ggtaaagccc
    21001 tcaccgaagc gagggcttga aggagaaggg ttatgatgcg acttgtcatc atactgattg
    21061 tactgttact cataagtttc agcgcttatt aacagtcagt cacaggggag gagcaatccc
    21121 cccttaccct tactcactaa attaggttaa agaatcaacg atgtcaatca gggcgatgca
    21181 ggtgtgtcgc cctcaccact cccagacttt cgacggtgta accaccgcag gcagtgggat
    21241 atcccactct tcaacgggga gtttttccac caactgacaa tcatgcgcat aacccaccgg
    21301 ttgcatttta tagtgctgcc agttttgtaa ggtccgatca taaaaaccac cgcccattcc
    21361 caggcgctga ccgtactcat caaaggcgac cagcggtgta atcaacacgt ctaatcggga
    21421 aaggggtagc acgtcacgca catccagttt tggctcatgg atcttcaact tgttcatcac
    21481 cagttcgctt tgtggatggt aattcaggaa cagcaaatta ccggcactaa agggatgcaa
    21541 aactggaaga tatacgcgct taccggcgcg ccagagttgt tctatgagtg gctgggtgtc
    21601 gagttcgcca tcaaaagaga ggaatacagc gaccgtatgt gccatcacca ccgggggata
    21661 agtcatcatc cgggtagcgg cttgttgacc catttcctgc tgttgttccg gcgttaacgc
    21721 acgacgacgt tgccgaatca ttttgcggat ttcttgtcgg gataatgtca gtgggagttc
    21781 tggtaattgc gtcatggctg gtgccagata agaagggaat ctccgagatg ccgccgcagg
    21841 ctgtaaccct tgaacccttg gttcaaggtg aatgtgtcgt cgcagtttta aggcttctcg
    21901 gacggaccga gcatgctcac caaccgcgga gcgccacatt cttgtggtat gaaatatcgg
    21961 ctcaggggac tggcccgctt gcgaacatct cagagaaatt ttgtcttcac ggttactcta
    22021 ccacagtaaa ccgaaaagtg tcattcaaag ttttggttag ttttttcggt gatgcgacct
    22081 tgttcaagta acgcttgttc tatggtctgc tgcagcatcc gaatacgctg ttccatactt
    22141 gccgcgtagt cacgagtctt cgctttttct tgcgctaact catagctgat attcagtgcg
    22201 gcaatgaaga ccaactgttc agtatttgtg actctagtgc gttctttcag atcttgcaac
    22261 cgttggttca gatcgtccgc tgcctgattc aacgcatccc tttggtcagg cgggcagttc
    22321 acacgcagtg aacggccaaa aatttggata tcgacgggtt gtgcagacat gccaccttcc
    22381 tgctgattga ctgcgctgcc ttcgtcttta gacccatggt ctgcgaaggg gcgacactat
    22441 agctaccctg atgagaagag acaagccctt ttctggtcca ccagggccca aagtggtagc
    22501 atatcatgaa tattcctccc tttgacgacg aatgcttatg tctatacaga acgaaatgcc
    22561 tggttacaac gaaatgaacc agtatctgaa ccaacaaggg acgggtctga cccctgctga
    22621 gatgcatggt ttaatcagtg ggatgatatg tggcggtaac gatgacagct catggctgcc
    22681 gctacttcac gacctgacga acgaaggcat ggctttcggt catgagctgg cacaggcact
    22741 gcgcaaaatg cactctgcca ccagcgatgc cctgcaggat gacggcttcc tttttcagct
    22801 ttatctacct gatggcgatg atgtcagcgt tttcgatcgg gctgatgcgc tggctggttg
    22861 ggtcaatcac ttcctgcttg gtcttggcgt tacgcaaccg aagctggaca aagtgaccgg
    22921 cgaaaccggt gaagccatcg acgatctgcg taacatcgcg cagttgggtt acgacgaaga
    22981 cgaagatcag gaagagcttg aaatgtcgct tgaagagatc atcgagtacg tccgtgttgc
    23041 cgcgctgtta tgccacgaca cctttactca tccgcaaccg accgcgccag aagtacaaaa
    23101 accgactcta cactaaaaac aaaaaacgta aggagagtgt tatgagtgat atatcccggc
    23161 aagaatttca gcgtcgccgt caggccctgg tagagcaaat gcaacccggc agcgccgcgc
    23221 tgatttttgc tgcaccagaa gtaacacgta gcgccgacag cgaatacccc tatcgtcaga
    23281 acagtgactt ctggtacttc accggcttta acgaaccgga agcggtgctg gtgctgatta
    23341 aaagcgatga cactcataac cacagcgttt tgtttaaccg cgttcgcgac ctgacggcag
    23401 agatctggtt tggtcgtcgc ttaggccagg atgccgcgcc agagaaactg ggcgttgacc
    23461 gcgcactggc attcagcgaa atcaatcaac aactttatca actacttaat ggtctggatg
    23521 tggtttacca cgcccagggc gaatatgcat atgctgatga aatcgtgaac agcgcgcttg
    23581 agaaactgcg taaaggttcg cggcaaaatc tcaccgcacc ggcaacgatg atcgactggc
    23641 gtcctgttgt tcatgaaatg cgcctgttca aatcaccaga agagattgcc gtacttcgcc
    23701 gcgcgggtga aatcaccgca ctggcgcata cacgggcgat ggaaaaatgc cgtccgggga
    23761 tgttcgagta ccatctggaa ggcgaaattc accacgaatt taaccgccac ggtgcgcgtt
    23821 acccttccta taacaccatt gttggcagcg gtgaaaacgg ctgcattttg cattacaccg
    23881 aaaacgagtg tgaactgcgt gacggtgacc tggtgttgat tgacgcgggt tgtgaataca
    23941 aaggttacgc tggcgacatt acccgcacct tcccggtcaa cggcaaattc actcaggctc
    24001 agcgtgaaat ctatgacatt gtgctggagt ctcttgaaac cagcctgcgc ctgtatcgtc
    24061 cgggaacttc cattcaggaa gtcactggtg aagtggtgcg catcatggtt agcggtctgg
    24121 taaaactcgg catcctgaaa ggtgatgttg atgaactgat cgctcagaac gcccatcgtc
    24181 ctttctttat gcatggcctt agccactggt taggactgga tgttcatgac gttggtgttt
    24241 atggtcagga tcgctcgcgt attctggaac cgggtatggt actgaccgta gaaccaggac
    24301 tgtatattgc gccggatgca gaagtgccag aacagtatcg cggtatcggc attcgtattg
    24361 aagacgatat tgtgattacc gaaaccggta atgaaaacct caccgccagc gtggtgaaaa
    24421 agccggaaga aatcgaagcg ttgatggcag ctgcgagaaa gcaatgagcg taatcatcgt
    24481 cggtggcggc atggcgggcg cgacgctggc gctggctatt tcccggttaa gtcacggggc
    24541 gctgccagta catttgattg aagcgaccgc gccagagtca catgcgcatc cgggctttga
    24601 tggacgagct attgcgctgg cggcgggtac ctgtcagcaa ctggcgcgca tcggcgtctg
    24661 gcaatctctg gctgattgcg caactgccat caccaccgtg catgttagcg atcgtggtca
    24721 cgctggattt gtcaccctcg ccgcagaaga ttaccaactg gcggcgctgg ggcaggttgt
    24781 cgaattgcac aatgtcgggc aacggctgtt tgcattgctg cgcaaagcac ctggcgtaac
    24841 gctgcattgc cctgatcgcg tggctaacgt tgcccgtact cagagccacg ttgaagtgac
    24901 gctggagagt ggcgagacgc tgacgggccg cgtgctggta gccgctgatg gcacccattc
    24961 agcgttagcc accgcgtgcg gtgttgactg gcagcaggag ccttacgaac aactggctgt
    25021 gattgccaac gttgcgactt ccgttgcgca tgaagggcgc gcttttgaac gctttacaca
    25081 atatggcccg ctggcgatgt tgccgatgtc tgacggacgc tgttcgctgg tctggtgtca
    25141 tccactggaa cggcgcgaag aggtgctgtc gtggagcgac gagaagttct gccgtgaact
    25201 ccagtcggcc tttggctggc gactggggca aattacccac gccggaaaac gtagtgctta
    25261 tccgctggcg ttaacccgcg ccgccaaacc tattacccat cgtaccgtgc tggtggggaa
    25321 tgcggcgcaa actctgcatc ccatcgccgg gcaaggattt aacctcggta tgcgtgatgt
    25381 gatgagtctt gcagaaaccc tgactcaggc gcaggaacgc agagaagaca tgggtgatta
    25441 cggcgtattg tgccgttatc agcagcgtcg acagcgcgat cgcgaagcaa ccatcggcgt
    25501 cacggacagc cttgtacatc tttttgccaa ccgttggacg ccgctggttg tcgggcgcaa
    25561 catcgggctg atgacgatgg aattatttac cccggcacgc gatgtgctgg cgcagcgcac
    25621 tcttggttgg gtggcgcgtt gaaggcggtt ttaaggaata aacaatgcaa agtgttgatg
    25681 tagccattgt tggcggcggc atggtcgggc tggcggttgc ctgcggctta cagggtatcg
    25741 gcttacgcgt tgccgtactg gagcagcgcg taccggaacc tctggcggcg gatgcaccac
    25801 cacaactgcg cgtttcggct atcaatgccg ccagcgaaaa attactcacc cgtcttggcg
    25861 tctggcagga cattctctct cgtagggcct gttgctatca cggcatggaa gtgtgggaca
    25921 aagacagctt tggtcacatc tcgtttgacg atcaaagcat gggctatagc catcttgggc
    25981 atatcgttga aaactcagtg attcactacg cgctgtggaa caaagcgctg cagtcgtcag
    26041 atatcactct gttggccccc gcagaattac agcaggtcgc ctggggtgaa aatgaaacct
    26101 tcctgacgtt gaaagatggc agcatgttaa cggcgcgtct ggtgattggc gcggacggcg
    26161 ctaattcctg gttgcgcaac aaagccgata ttccgctgac tttctgggat tatcagcatc
    26221 acgcgctggt ggcgaccatt cgcacggaag aaccgcatga tgcggtggcg cgacaggttt
    26281 ttcatggcga aggcattctg gcctttttgc cgcttagcga tccgcatctt tgctcgattg
    26341 tctggtcact gtcgccagag gaagcgcagc ggatgcagca ggcaggcgaa gacgaattta
    26401 atcgcgcgtt aaatatcgct tttgataatc gcctgggctt atgcaaggtt gaaagtgagc
    26461 gcctggtgtt cccgctaacg gggcgttatg cgcgccagtt tgccgcgcac cgtctggcgc
    26521 tggtgggcga cgccgcgcat accattcacc cgttggcggg gcagggggta aatctcggct
    26581 ttatggatgc tgcagagctg attgctgaac tgaaacggtt gcatcgtcag ggtaaagaca
    26641 tcgggcagta catttacctg cgtcgctatg agcgtagccg caagcacagc gcggcgctga
    26701 tgctggctgg tatgcaggga ttccgcgatc tgttttccgg ggctaatccg gcgaaaaaat
    26761 tgctgcgtga tattggtctg aaactggctg atacgcttcc tggcgttaag ccacaactta
    26821 ttcgccaggc aatgggatta aacgatttgc ctgaatggct gcgttaaaaa tttctcttct
    26881 gctgtttatt tgatacccat cacactttca tctcccggtt ttttcgccgg gagattttcc
    26941 tcatttgaaa taaa
//
LOCUS       DACADF010000061        26939 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.061, whole genome shotgun sequence.
ACCESSION   DACADF010000061 DACADF010000000
VERSION     DACADF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.061"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            102..2282
                     /gene="katG"
                     /locus_tag="ISB30_002366"
     CDS             102..2282
                     /gene="katG"
                     /locus_tag="ISB30_002366"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAP1989993.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            2376..3281
                     /gene="yijE"
                     /locus_tag="ISB30_002367"
     CDS             2376..3281
                     /gene="yijE"
                     /locus_tag="ISB30_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAP1989994.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(3308..3925)
                     /locus_tag="ISB30_002368"
     CDS             complement(3308..3925)
                     /locus_tag="ISB30_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAP1989995.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(4201..5304)
                     /gene="gldA"
                     /locus_tag="ISB30_002369"
     CDS             complement(4201..5304)
                     /gene="gldA"
                     /locus_tag="ISB30_002369"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HAP1989996.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(5315..5977)
                     /gene="fsa"
                     /locus_tag="ISB30_002370"
     CDS             complement(5315..5977)
                     /gene="fsa"
                     /locus_tag="ISB30_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP1989997.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(5989..8490)
                     /gene="ptsP"
                     /locus_tag="ISB30_002371"
     CDS             complement(5989..8490)
                     /gene="ptsP"
                     /locus_tag="ISB30_002371"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP1989998.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            8799..9878
                     /locus_tag="ISB30_002372"
     CDS             8799..9878
                     /locus_tag="ISB30_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAP1989999.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            9893..10213
                     /locus_tag="ISB30_002373"
     CDS             9893..10213
                     /locus_tag="ISB30_002373"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAP1990000.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            10264..12561
                     /locus_tag="ISB30_002374"
     CDS             10264..12561
                     /locus_tag="ISB30_002374"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP1990001.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGPRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVLLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGAVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            12527..13405
                     /locus_tag="ISB30_002375"
     CDS             12527..13405
                     /locus_tag="ISB30_002375"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAP1990002.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIKQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            13407..13748
                     /locus_tag="ISB30_002376"
     CDS             13407..13748
                     /locus_tag="ISB30_002376"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAP1990003.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(13735..14586)
                     /locus_tag="ISB30_002377"
     CDS             complement(13735..14586)
                     /locus_tag="ISB30_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1990004.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(14812..16545)
                     /gene="cptA"
                     /locus_tag="ISB30_002378"
     CDS             complement(14812..16545)
                     /gene="cptA"
                     /locus_tag="ISB30_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAP1990005.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(16728..19379)
                     /gene="ppc"
                     /locus_tag="ISB30_002379"
     CDS             complement(16728..19379)
                     /gene="ppc"
                     /locus_tag="ISB30_002379"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAP1990006.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(19681..20832)
                     /gene="argE"
                     /locus_tag="ISB30_002380"
     CDS             complement(19681..20832)
                     /gene="argE"
                     /locus_tag="ISB30_002380"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAP1990007.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQSVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIMGQSGHSSDPSRGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHKLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            20986..21990
                     /gene="argC"
                     /locus_tag="ISB30_002381"
     CDS             20986..21990
                     /gene="argC"
                     /locus_tag="ISB30_002381"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAP1990008.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDIGEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            21998..22774
                     /gene="argB"
                     /locus_tag="ISB30_002382"
     CDS             21998..22774
                     /gene="argB"
                     /locus_tag="ISB30_002382"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAP1990009.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSIKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            22835..24208
                     /gene="argH"
                     /locus_tag="ISB30_002383"
     CDS             22835..24208
                     /gene="argH"
                     /locus_tag="ISB30_002383"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAP1990010.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYSNATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSLVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            24475..25392
                     /gene="oxyR"
                     /locus_tag="ISB30_002384"
     CDS             24475..25392
                     /gene="oxyR"
                     /locus_tag="ISB30_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAP1990011.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(25375..26775)
                     /gene="sthA"
                     /locus_tag="ISB30_002385"
     CDS             complement(25375..26775)
                     /gene="sthA"
                     /locus_tag="ISB30_002385"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAP1990012.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         6100 a         6848 c         7511 g         6480 t
ORIGIN      
        1 atatgtaaga tctcaactat cgcatccgtg gattaattca attataactt ctctctaacg
       61 ctgtgtatcg taacggtaac actgtagagg ggagcacatt gatgagcacg tcagacgata
      121 tccataacac gacagccacc ggcaaatgcc cgttccatca gggcggtcac gaccagagcg
      181 caggtggggg cacaaccacg cgcgactggt ggccaaatca actccgtgtt gacctgttaa
      241 accaacattc taatcgttct aacccgctgg gtgaggactt tgactaccgc aaagaattca
      301 gcaaattaga ttactacggc ctgaaaaaag atctgaaagc cctgctgaca gaatctcagc
      361 cgtggtggcc tgccgactgg ggcagctacg ctggtctgtt tattcgtatg gcctggcacg
      421 gcgcgggtac ttaccgttca atcgacggtc gcggcggcgc gggtcgtggt cagcaacgct
      481 ttgcaccgct gaactcctgg ccggataacg taagcctcga taaagcgcgt cgcctgttgt
      541 ggccaatcaa acagaaatat ggtcagaaaa tctcctgggc cgacctgttt atcctcgcgg
      601 gtaacgtggc gctggaaaac tccggcttcc gtaccttcgg ttttggtgcc ggtcgtgaag
      661 acgtctggga accggatctg gatgttaact ggggtgatga aaaagcctgg ctgactcacc
      721 gtcatccgga agcgctggcg aaagcaccgc tgggtgcaac cgagatgggc ctgatttacg
      781 ttaacccgga aggcccggat cacagcggcg aaccactttc ggcagcagca gctatccgcg
      841 cgaccttcgg caacatgggc atgaatgacg aagaaaccgt ggcgctgatt gcgggtggtc
      901 atacgctggg taaaacccac ggtgccggtc cgacatcaaa tgtaggtcct gatccagaag
      961 ctgcaccgat tgaagaacaa ggtttaggct gggcgagcac ttacggcagc ggcgttggcg
     1021 cagatgccat tacctctggt ctggaagtgg tctggaccca gacgccgacc cagtggagca
     1081 actatttctt cgagaacctg ttcaagtatg agtgggtaca gacccgcagc ccggctggcg
     1141 caatccagtt tgaagcggta gacgcaccgg aaattatccc ggatccgttt gatccgtcga
     1201 agaaacgtaa accgacgatg ctggtgaccg acctgacgct gcgttttgat cctgagttcg
     1261 agaagatctc tcgtcgtttc ctcaacgatc cgcaggcgtt caacgaagcc tttgctcgtg
     1321 cctggttcaa actgacgcac agggatatgg ggccgaaatc tcgttacatc gggccggaag
     1381 taccgaaaga agatctgatc tggcaagatc cgctgccgca gccgatctac aacccgaccg
     1441 agcaggacat tatcgatctg aaattcgcga ttgcggattc tggtctgtct gttagtgagc
     1501 tggtatcggt ggcctgggcg tctgcttcta ccttccgtgg tggcgacaaa cgtggtggtg
     1561 ccaacggtgc gcgtctggca ttaatgccgc agcgtgactg ggatgtgaac gccgcagccg
     1621 ttcgtgctct gccagttctg gagaaaattc agaaagagtc tggcaaagct tcgctggcgg
     1681 atatcatcgt gctggcaggt gtggttggcg ttgagaaagc cgcaagcgcc gcaggtttga
     1741 gcattcatgt accgtttgcg ccgggtcgcg ttgatgcgcg tcaggatcag actgacattg
     1801 agatgttcga actgctggaa ccaattgctg acggtttccg taactatcgc gctcgtctgg
     1861 acgtttccac caccgagtca ctgttgattg ataaagcaca gcaactgacg ctgaccgcgc
     1921 cggaaatgac tgcgctggtg ggcgggatgc gcgtactggg tgccaacttc gatggcagca
     1981 aaaacggcgt cttcactgac cgcgttggtg tattgagcaa tgacttcttc gtgaacttgc
     2041 tggatatgcg ttacgagtgg aaagcgaccg acgaatcgaa agaactgttc gaaggccgtg
     2101 accgcgaaac tggcgaagtg aaatacacag ccagccgtgc ggatctggtc tttggttcta
     2161 actctgtcct gcgtgcggtg gcggaagtct acgccagcag cgatgcccac gagaagtttg
     2221 ttaaagactt cgtggcagcg tgggtgaaag tgatgaacct cgaccgtttc gacctgctgt
     2281 aatctgacct tcttcagcga ctgcctttca tgcagtcgct gaagtttctt taccggcgta
     2341 tagtgtccac aggaaaacta cacactggat ctctcatgtc tgccgcagga aagagcaacc
     2401 cactggcaat cagtggcctg gttgtgctca cacttatctg gagttatagc tggattttca
     2461 tgaagcaagt caccagctac atcggtgcct tcgacttcac cgccttacgc tgcattttcg
     2521 gcgctctcgt tttattcatc gttcttttat tacgtggtcg cggaatgcgc ccgacgccgt
     2581 ttaaatacac cttagccatt gccctgttac aaacctgcgg gatggttggc ctggcacagt
     2641 gggcgctggt cagcggcgga gcggggaagg tggcaatcct gagctatacc atgccgttct
     2701 gggtggtgat tttcgccgcg ttgtttctcg gtgaacgcct gcgacgtggg caatatttcg
     2761 cgatactgat tgccgctttc ggcttatttt tggtgttgca gccgtggcaa ctcgatttct
     2821 cttcgatgaa aagtgccatg ctggcaatcc tctccggtgt gagttggggg gcgagcgcga
     2881 ttgttgctaa acgtctgtat gcccgccatc cgcgcgtgga tttattgtcg ctaacatcct
     2941 ggcagatgct gtatgcggcg ctggtgatga gcgtggtcgc tttactggtg ccgcaacgtg
     3001 aaattgactg gcaacccacc gtgttctggg cgctggccta cagtgcgatt ctggcgacag
     3061 cgctggcgtg gagcttatgg ttgtttgtat tgaaaaactt gcctgccagt attgccagct
     3121 taagcacact ggccgttccc gtttgcggcg tactcttttc ctggtggctg ctcggtgaga
     3181 atccgggggc agttgaaggt agcggtattg tgctgattgt actggcactg gcgctggtga
     3241 gccgtaagaa aaaagaagcc gtcagtgtaa aaaggatctg aattttttct tcatgtgggg
     3301 agatctctta tttagcaaaa taccgatagt gccccaccat ccgccagcta aacagtacat
     3361 cttcttcctg cgcgcctgcg ccaatgttat gtatcaccag cggcgtaccg tcgcgggcga
     3421 agccatctga caccacccca atatgtgcca gcccgttatc taatcgccag gagacaatat
     3481 cgcccgcttg atagtcactg gggttcttgc tggtggggcg tgttttatcg tggcgggtaa
     3541 accaggtttc cagattaggc acccgacggt gatcgatgtt gctgtccggg cgctttaact
     3601 gccatttttg cgggtacgca gcaaaattct tcgccatatc ttcgtgcacc agtttctgca
     3661 aatcgacctt ctggctgcgc aatgcacgga tcaccacatc ggaacatacg ccgcgttctt
     3721 gcgggacatc accgccagga taggtaagct gcacatacgc cggatcgtaa aatagcgtgc
     3781 tgccgatttg ctgtctggcg ccgtctgcga tggcaagatt ggtgttggcc tggatttgta
     3841 ccacggttgg tggaacggcg ggagatttta aggagtaact ggtaaatacc gtcagaagac
     3901 tgaataacgc cagtgaagct ttcatctgtt tcgtccgtga aaataagagt cacagattcg
     3961 accttccggg ctaacgattg ttctgtggag aaaatgtcca gaagcggata aaaaaacacc
     4021 cgaacaacgt ggaagtttca gattactggc acagcgtgcc ggatgcggcg tgaacgcctt
     4081 atccggtcta caaaagtacg taaatttact atattgcaga gattgcgtag gcctgataag
     4141 cgtagcgcat caggcaattt tgcgttcaaa ctcccggaca agccgggagt ttggagtaga
     4201 ttattcccac tcttgcagga aacgctgacc atactggtcg gctaccagca gtgcggcgta
     4261 aacctgatct ggcgtcgcgc cgccaggcat gttgtggatg gtttcacctt ctgcacatgc
     4321 cgcttctgcc acaattcgca tttttgccgg gacatcttct ttaatatcca gttgagcgag
     4381 agttattggc aaacctaccg catggctaag cgcagctacg gtttcgattt cctccaccgg
     4441 cgcgttttcc agaaccagct gcgtcagcgt accgaatgcc actttttcac catgataata
     4501 gtgatgcgcg tccgggatag cggtcaggcc gttatgtacc gcgtgtgccg ccgccagccc
     4561 gccgctttca aaaccaacgc cactcaaata ggtgttcgct tcaatcacgc gctccagcgc
     4621 cggagtcact acatgctgtt cggcggcaag catcgctttt tcgccttctt ccagcagggt
     4681 gttgtagcac agttcagcca gtgccagcgc tgcctgagtg cacttgccac ccgccatggt
     4741 ggtcgcgccg ctacgggaac aggcacgcgc ttcaaaccag gttgccagcg catcgccaat
     4801 acccgccgct aacagacgcg cgggcgcgcc agcgacgatt ttggtgtcga caatgaccat
     4861 attcgggtta tttggcaaca gcagatagcg gtcaaactca ccctcatcgg tgtagataac
     4921 agacaatgcg ctgcacggtg cgtcggtaga agcgatagtc ggtgcgatcg ctaccggaac
     4981 acccatgaaa tgtgccagtg ctttggcagt atcgagggtt tttccgccac caataccgag
     5041 aattgcgcca cactgcgcag tctccgcgat gccacgcaga cggtcgatct cattttgcga
     5101 acattcaccg ccaaacggcg caatttctac taccagtcca gcatctttaa agcttttctc
     5161 gacagtggat tgagcaaaac ctaaaacaaa tttgtcaccc accactaacc agcgttccgc
     5221 cagtggcttc aggtactcgc ccagacgatt aattacatct gcgccctgga tgtatttacc
     5281 tggtgattga ataatgcggt ccataattac tcctttagag atgagtagtg ccaaatgcgg
     5341 cattccagtc gtgctcgaac ttctctatag ctgactctac cgcaggggtg ttaagcattt
     5401 gttgcgctac atctaagggc agtgtgatgg attcacatcc tgccagcaaa caatccagcg
     5461 cctgacgtgg tgttttaaag ctggcagcca gcaccatgct ttctggcgca tgcatttcca
     5521 gtaacgcttg caactcctgt acagtacgaa tgccgtcacc gccctgggca tctacgcggt
     5581 taacgtatgg agcaacgtat ttggctccag ccagcgccgc cagtaatcct tgcgcggcgc
     5641 tgtacactgc ggttcccagc gtagtaatgc cttctttctt cagcatttta attgctgcca
     5701 gaccttcaga ggttaccgga attttcacca caatgcccgg aatagcgtcg cgcaggcgtt
     5761 tcgcttcttt caccataccc tgcgcgtcgc ggctcatggt ctgagcaaac agaatgccct
     5821 catcaccgat cgctttttgc aggcgcggca gcacttccca gatggactcc ttgctggcag
     5881 cgataatgct cgggttagtt gtcaccccgg caatcgggaa tatgcgtgcc agacgttcga
     5941 cttctgcgac gttagcggtg tccagataca gttccatgat gtttcctttt acagttccag
     6001 ttcatgttgc agcaggctgg cgatagcgtc ggcagaggcg gcgttaacca gcgcattacg
     6061 gaattcttcg tgcatgatgc gacgagcgag gcgcgagaaa atgcgcatat gttgatcgcc
     6121 cgcggcgtgt ttgttcaggg ttaacataat gatgaattgc gcttcatcat cgccccagcg
     6181 cactggcgct tgcagacgcg ctacgctgat ggtggattgc tcaatgtgtt ccgatttact
     6241 gtggggaatg gcgaaactaa agcccagtcc ggtagagaaa acggcctcgc gcgcccacaa
     6301 gtcggcttcc agtttacgcg gatagcggca gcggcccgcc agcagcaggt tatcggtcat
     6361 gcctttgagc acttcttctt tgctgcgcca gtcgctttcc agtgtgatgc actcggcggt
     6421 gaccagcggt gcgtcctgtt gggtcatgcg gaattgcgcc agcaggtgtt ccacttccag
     6481 cgaagtacgg caggccattg cctggttgag caactggcgg cactcacggc tatcaagttg
     6541 cgccatccgc gctttcgccg ccggaattga tggtgcgctc atgctgagtt catccagccc
     6601 taaaccgacc agcaacggca gcacggaacc tttcgctccc agttcaccgc acagaccaat
     6661 ccatttgccc tggcgatgca ccgcctgcac ggcgtaatcg agcgcccgca agaaggccgg
     6721 attcaggctg ttgtagtgac gagtcacctt agcgttatcg cgatccactg ccagtagata
     6781 ttgcgtcagg tcattactac caatactaaa gaaatcaatc tcttcgcagc attgatcgat
     6841 gatgaacatc accgacggca cttccagcat gatgccgagc tggatcttct catcaaacgg
     6901 aatgtgttcg ttacgcagtt gctgtttggc ttccgccagt ttttctttca cccataagat
     6961 ctcttccatt gaggagatca tcgggatcat gattttcagg ctgccgtgag cagaggcgcg
     7021 caggatcgat cgtagttgtg tggtaaacaa cgacgcgtat tcttcataaa tacgcaccgc
     7081 gcgataaccg aggaacgggt tagcttctgc gggaatgttc agataatcaa cgggtttgtc
     7141 accaccaatg tccatagtgc gcacaataat gctgcgtccg ttggcagact ccagcgcctg
     7201 acaaaagatg ttgtacagct cgctttcacc cggtgcactg gtgcgatcca tatagagcat
     7261 ttcagtgcgg aacaaaccaa cgccttccgc accattgccg aatgcggcct gcgcttccac
     7321 ggagtgagcg atgttagcgg caatttcaat gcggataccg tcagcggtac gcgcttgctg
     7381 ggtcagccag acacgctgtt gctcacgcag agcgtcctgt acgcgggctt cttgctgata
     7441 ataacgggcc acggattccc ctggctcaac cacaatcgcc cctgcgttgc cgtcgatata
     7501 aatcgtctga tgctgccacg gagtaagggc atcaatatcc acacctacca gcgtcggaat
     7561 gttgaacgaa cgggcaagga tcaccgtatg tgatgtggtg ccgccacttt tcagtaacag
     7621 acctttgaga tgatttttat ccagttcgag gaactggctg ggggtcagtt catcagccat
     7681 acaaatggcg ggctgcgtca gtttgcccgg tgccgggaag cgttgctcac cgtagatttg
     7741 ctggagtaac tggaagcaga cgtcgcgtac gtccagggca cgttcttgca ggtaactgct
     7801 gctggaacgg gcgaactctt cgcaaaagtg attcgcactg gcaacaatcg cttcggcgca
     7861 gcttaatccg gcgctgacac ctgccagtaa atgttcgcgc agggaagtat cgccagccag
     7921 ggatcggtgc gcttccagaa tcgcgctggt agcaccgtcg ctatccagca gacgaaactc
     7981 tatgtttttc agtaccagcg tcagaccgtt ttccagtgcg gattgttcgg cgtcaacgcc
     8041 tttggctgcg ggaagattac ccagcgcatt gagatctaaa gaagagatcg gcgtcagaat
     8101 gccgcctgcg ctaccgctgc acacggtgcg agcgcggata atttgcggat tcagattggt
     8161 cagtgaaacc ggcagtggtt ccagttcgtc agattttact tccgccagcg gtgcgtcgca
     8221 gtgggggaat tcatcgcgca gccattggct taaacgctgg tgcgcttcct gttcgtcggc
     8281 cccggaaatc agtaactggc agttatcgcc caccagcgta tcggtgccaa tcagcgcaag
     8341 ggcgcttttg gcgttgccct tgcggtcagt gcgcaggtta tgccactcaa tttgtgatga
     8401 aaaagtatta cacagcgttt caacgtggct tgccggacgc gcatggacgc cgttaggtag
     8461 ctcacaaata aattccacaa tcagggccat tgcctttctc ccggtacggt tttctgttta
     8521 caggataaga gcgcacggca acggcctgcc atgtgacaaa tctgccaaaa gctggacaaa
     8581 tgtaatgtaa ccgtcaattt gcgacgcgtc tcacaagacg ccgttttgcg gcgtacttcc
     8641 ggattatcgc aagttatgag gcggatcgca tttttgtact gatattacaa aagtccagta
     8701 aatggccttt ttatccactg tttgctccgc ctgcgattgc ctattgtttc ttccatcaaa
     8761 aatatggaca tgggctttcg caagcccggg agcagcttat gaatgagttg gtgcagatcc
     8821 tgaaaaatac ccgtcagcat ttaatgacgg gcgtttcaca catgattccc ttcgtggtat
     8881 cgggcggtat tttgctggcg gtttccgtca tgttgtatgg caaaggcgca gtgccggatg
     8941 ccgtagccga tccgaatctg aaaaaactgt ttgatatcgg cgttgcaggt ttgacgctga
     9001 tggtgccttt cctcgccgct tacatcggtt attccattgc agaacgttct gcgctggctc
     9061 catgcgctat cggtgcctgg gttggtaaca gctttggcgc gggcttcttt ggtgcgctga
     9121 tcgccgggat tatcggcggc atcgtggtgc attacctgaa gaaaattcca gtgcataaag
     9181 ttctgcgctc ggtaatgcca atcttcatca ttccaatcgt cggcacactg attaccgctg
     9241 gcgtcatgat gtgggggctg ggcgagccgg tgggggcgtt gaccaacagc ctgacccagt
     9301 ggcttcaggg gatgcagcag ggcagcattg ttatgctggc ggtgatcatg ggtctgatgc
     9361 tggcgttcga tatgggcggc ccggttaaca aagtggccta tgccttcatg ctgatttgcg
     9421 ttgctcaggg tgtttatacc gtggtggcta ttgccgctgt tgggatttgt gttccaccgc
     9481 tggggatggg gctggcgacg ctgattggtc gtaaaaattt ctccgcagaa gagcgcgaaa
     9541 ccggcaaggc ggcactggtg atgggctgtg ttggcgttac tgaaggggcg attcctttcg
     9601 ccgctgccga tccgctgcgt gtcattcctt ccatcatggt cggttcagtt tgtggtgcgg
     9661 taactgcggc gctggtcggt gcgcagtgct atgcaggctg gggtggtctg attgtgctgc
     9721 cggtggttga aggtaagttg ggttacatcg cggcagtggc tgtcggggcg gttgtgacgg
     9781 cagtctgcgt gaacgtgctg aaaagtctgg cgcgtaaaaa tgggtcttca actgaagaga
     9841 aggaagacga cctggatctg gattttgaaa ttaattaatt gaggaacgag ccatgacgaa
     9901 aattatcgca gtaaccgcat gtccttcagg tgttgcccat acttatatgg cggcagaggc
     9961 gctggaaagc gccgcgaaag cgaaaggctg ggaagtaaaa gtagaaaccc aggggtcgat
    10021 tggtctggaa aatgaactga ctgcggaaga cgtggcaagc gccgatatgg tgattttgac
    10081 caaagatatc ggcatcaaat ttgaagagcg ttttgctggc aagactatcg tgcgtgtcaa
    10141 catcagcgac gcggtaaagc gtgctgacgc cattatgagc aaaattgaag ctcacctggc
    10201 acaaaccgct taagttctta tcccgcccgc aagggcgggt tcgctttccc acaggagttc
    10261 ctcatgacga atcgtatctc tcgcctcaaa actgcactgt ttgccaatac ccgtgaaatc
    10321 tcgctggagc gggcgttgct ttataccgcc agccatcggc aaaccgaagg ggagccggtg
    10381 ataatgcgtc gggcgaaagc aacagcgtat atccttgaac acgttgaaat ttcgattcgt
    10441 gatgaagaac tgattgccgg taaccgcacc gtaaaaccac gcgccgggat tatgtcgccg
    10501 gaaatggacc cttactggct gctgaaagaa ctggatcaat tcccgacgcg tccgcaggac
    10561 cgctttgcta tcagcgaaga agataaacgt atctaccgcg aagagttgtt cccgtactgg
    10621 gaaaaacgtt cgatgaaaga tttcatcaac gggcagatga cggatgaagt aaaagccgcg
    10681 accagcacgc agattttcag catcaaccag acggataaag gccaggggca cattattatt
    10741 gattacccgc gcctgctgaa tcacgggctg ggggagctgg tggcacagat gcagcaacat
    10801 tgtcagcaac agccggagaa tcacttttat caggcagcgc tgttactgct ggaagcctcg
    10861 cagaaacata ttttgcgtta cgccgaactg gcggaaacga tggcggcaag ctgcactgat
    10921 ggcccgcgtc gcgaagaatt gctgactatt gcggagatct cccgccataa cgccgaacat
    10981 aagccgcaga cgttctggca ggcgtgccag ttattctggt acatgaacat cattctgcaa
    11041 tacgaatcca acgccagttc gctatcgttg gggcgcttcg accagtatat gttgccgttc
    11101 tatcagacat cattaaccca gggcgaagat ccggcattcc tgaaagaact gctcgaatct
    11161 ttatgggtga aatgcaacga catcgtgctg ttgcgctcca ccagtagcgc gcgttatttc
    11221 gccggtttcc ctaccggcta taccgcactg ctcggcgggt taaccgagaa cggacgtagc
    11281 gcggtgaacg tgctttcgtt cctttgcctt gacgcttatc aaagcgtgca attaccgcaa
    11341 ccgaacctcg gcgtgcgcac taacgccttg atcgacacgc cgttcctgat gaaaaccgca
    11401 gaaaccattc gcctcggcac cggtattccg caaatcttta acgatgaagt ggtagtgcca
    11461 gcgttcctca accgtggcgt tttgctggaa gatgcgcgcg actattccgt agtgggctgt
    11521 gtggaattat ctattcccgg cagaacctac ggcttgcatg acatcgcgat gtttaacctg
    11581 ctgaaagtga tggaaatctg cctgcatgaa aatgaaggca acgctgcgct gacttatgaa
    11641 ggtttactgg aacagatccg cgccaagatc agccactaca tcaccctgat ggttgagggc
    11701 agcaatattt gtgatatcgg ccatcgcgac tgggcacctg taccgctact ctcgtctttt
    11761 atcagcgatt gtctggaaaa aggtcgcgat attaccgatg gcggcgcgcg ttataacttc
    11821 tccggcgtac aggggatcgg tatcgccaac ctgagcgatt ctctccatgc gttgaaaggg
    11881 atgatttttg atcaacagcg tttaagtttt gacgaattgc tgtcggtatt aaaagccaac
    11941 ttcgccacgc cagaaggcga aaaagtccgc gctcgcttaa ttaaccgctt cgagaaatac
    12001 ggtaacgata tcgacgaggt ggataacatt agcgccgaac tgttacgcca ctactgcaaa
    12061 gaagtggaaa aataccagaa cccgcgcggc ggctacttca cgccgggatc gtataccgtt
    12121 tctgctcatg tcccgttggg agcggtggtt ggcgcgacgc cagacggtcg ctttgccgga
    12181 gagcaactgg cagacggtgg cttgtcaccc atgctgggtc aggacgcaca aggaccaacg
    12241 gcggtactga aatcagtcag taagctcgat aacacactgc tttctaacgg tacattgctg
    12301 aacgtgaaat tcactccggc gaccctcgaa ggtgaagcgg gattacgcaa actggccgac
    12361 ttcttacggg cgtttaccca gcttaagttg cagcatattc agtttaacgt ggtgaacgcc
    12421 gacacgttac gggaagcgca acagcgccca caagattatg ccgggctggt ggtgcgcgtt
    12481 gccggataca gcgccttctt tgtcgaactg tcgaaggaga tccaggatga catcatccgc
    12541 cggacagcgc atcagctgta acgttgtgga aacgcgccgc gatgatgtgg cgcgcatttt
    12601 caacattcag cgttattcac tgaatgacgg cgagggcatt cgtacggtgg tcttttttaa
    12661 aggctgtccg catctttgcc catggtgtgc taatccggag tcgatctccg gcaaaatcca
    12721 gacggtgcgc agagaggcga aatgtctgca ctgcgcgaaa tgtttgcgtg atgcggatga
    12781 atgcccctcc ggggcatttg aacggattgg ccgcgatatc agccttgacg cgctggaacg
    12841 ggaagtgatg aaagatgaca ttttctttcg cacgtccggc ggcggcgtca cgctttctgg
    12901 cggcgaagtg ttaatgcagg cggagtttgc gacccgtttt ttacagcgac tgcggctatg
    12961 gggtgtgtca tgcgccatcg aaactgccgg agacgcgcca gccagcaagc tgttaccgct
    13021 ggcgaaattg tgcgatgaag tgttgttcga tttaaaaatt atggatgcgg ctcaggcgcg
    13081 ggatgtggtg aagatgaacc tgcaacgcgt gctggagaat ctgcgtttgc tggtgagtga
    13141 gggtgtcaac gtgatcccgc gtttaccgct gatccctggt ttcacgctca gccgggagaa
    13201 tatgcagcag gcgctggacg tgctgatccc gctgaatatc aagcagatcc atctgttacc
    13261 gtttcatcag tacggcgagc cgaaataccg cctgctgggg aaaacatggt cgatgaaaga
    13321 ggttgctgcg ccgtcgtcag ccgatgtggc aacgatgcgt gaaatggcag aacgggccgg
    13381 atttcaggtt accgtgggag gttaaaatgg catatctggt ggcagtaacc gcctgcgtaa
    13441 gcggcgtggc gcatacttat atggcggcgg aacggctgga aaagttgtgc cagttagaga
    13501 agtggggcgt cagcattgaa actcagggcg cgttgggaac ggagaatcgt ttagcggacg
    13561 aggatatccg ccgggcggat gttgcgctgt taattacgga tatcgagctt gccggtgctg
    13621 agcgatttga acattgtcgc tacgtgcaat gcagcatcta cgctttcctg cgtgagccgc
    13681 agagggtgat gagcgcagtg cgcaaagtgc tttctgcgcc gcagcaaacc catcttattc
    13741 tggagtagtc ggtttttctg tcagttggct gtggtactgc cgacgatatt ccgacggcga
    13801 gcgttcggta tttttacgaa acagacggca gaagtagttg ctgtcgacaa agccgcaggc
    13861 gtgcgccact tcttttacct tcaggtcata gcctttcaat aatgtcttag cgtgctccag
    13921 tcgcgtgtga ttcaggtatt cgttaaaacc aatggccccc gttttttgaa acaggtgcga
    13981 gaggtaattt ggcgaaatat agaacgcctg tgcgacagat tcgcgggtaa gtgcggaggc
    14041 atagcgttcg tcgatataat cacgaatagc ttcgaataga gcctggctgc gtgaggcggt
    14101 ctgtatttgg ctgccgagca aatcgcggca gtggctaagc aggctggcga cgataagtct
    14161 ggccgtttgc tgctcctgcg actgcatttg catttcattg agcgtttgta gcagaaaaga
    14221 accaatgcgt gggccgcgtc gggcgacgtg ttgcttcgcc agattttgat attgtttgcc
    14281 atcccattgc acgacgctga agccgagttg ctgtttgcca aatagcacgc taaaggtcgt
    14341 ggcgggggct tgccattgtg gaaaattcca gccaccagca gggacgtaca gcacatcgcc
    14401 gggaactaac gctgctccag cgccggtatc aataaactca ccttccagca caatttccag
    14461 acgtggaaaa tcgacctgat acgcaagatc gggcaccggg ccgttgctgc tggcaaagta
    14521 aatctggcgc agggacagcg ggccgttgat caggcgggag agcagatagc tgacgtcgtg
    14581 atacatgtta ttcccttagg tatgtagacg ggaaaacagt aactggcatt atgaaaaaag
    14641 gccacctgaa aagtgtggcc tgatgggagt gagatgtaaa cattgcggca gtttcagtcg
    14701 acttgtattg ccggatgcgg cgtaaacgcc ttatccggcc taccgccgcg atctgtaggc
    14761 cggataagac gcgtcaagcg tcgcatccgg caaataacat ctggctgata attactgatt
    14821 acccacctga tcgccatacg gcagagtgtc gtaatcgatc agtgcgtttt tcttatacgg
    14881 gttaccaatc cagcgggtag tttctttgaa ctgcggattc accactgaac gggttggatc
    14941 gtaaccgtcg taagataagc ccgccaaatc tgaccaggtg tggatcagtt ccgccaggct
    15001 gtatttacga tcaacatcct gtgagaaatc acgcggatga gtcgcttgcc atttttctga
    15061 cgtccacagc aggaacggaa tggtgtacat atgacgcgtc ggattgtctt cattacgccc
    15121 ctgagttttg tgcggcggcg tgtcgtaaac ctcttcaccg tggtcagaga aataaaccag
    15181 gaaaccgttc ggatcggtcg ctttaaagtc tttaatcagg ctggcaacca cgtgatcgtt
    15241 gtacaggtta gcattgtcat aatcattata tgactccagc tcttctgcgt ttaatcccgg
    15301 tggaacatgc tctgtattac cgtcaaattt gccctgatct tccggataac ggtacttgta
    15361 cttgatatgc gtacccagca gatgaacgat gatcagtttc ttcggcgcag ggtccttcag
    15421 cacttcctgg aacggcttca gcacgttggt gtcgtattca cgcgcactct gcgtacgttg
    15481 ctggttcatg tagtactgct tgtcggtctg gcgcgaaaat accgtcagca tcgtattgcg
    15541 ggcggtcatt gtctgctggt tggtgatcca gaaggtttta taacccgcct gtttcatcat
    15601 gttcatcagc gacggctgcg tcagatacag atccgggttc ttttcattgg caaaggtcag
    15661 cgcctgttgc aggatttcaa tggtgtacgg acgagacgta accacgttat taaacacggt
    15721 cagattcgga tcggttttat gcagcgcatc cagctccggc gtggtttcac gcggataacc
    15781 gtacaggctc atgcgtccac gctgggtcga ttcgccaatt accagcacta aggtgcgcgg
    15841 ttcgttaccc gattcatctt tgaaattagc cagcggcggc aatgcattat tttcattcag
    15901 tagctttgtt agcgagttta gttgctgacg atactgataa tagccggtca ggaattgcca
    15961 cggtgcggca ggctccatac gcgatgccag gttatccaac gttttctcaa acggcttgtt
    16021 tttgataaac gtattcatgg cgatcggatg cagaatcaag ccataaagta gggcaaaaga
    16081 gacaacataa cgccacggct ttggaatata gaccgggcgc aggcgtgtcc acagcagaac
    16141 tgccaccgcc gtataggcca gcgcgataag cacaattttc aggctgaaat actggcttaa
    16201 atactcgctg gcttcgttgg tgttggtttc gaacatcaca aacagaacgc tctgcgagaa
    16261 ctcctgaccg tagatgacgt aatagcacag cgccgccaga gaggccgccc atagcaccac
    16321 gccgatcact gcggcaataa ttttaatccg cttcggaaag aggaataccg ggatcaacca
    16381 cagcgaactg aataacagcg agtcgcgaat gccgttagtg ccactataac cactgatgta
    16441 aataatggcc tgtagcagag tagagaaaaa ccaaaagtag agcagtgccc aacccagggc
    16501 tttccagcta aaaaggggtt tagcctggac ttctgtggaa tgcatagtaa gaacctgtct
    16561 tgaaaaaaat atcgccgaat gtaacgacaa ttccttaagg atatctgaag ggatatccag
    16621 attttgaata aaatgcagac agaaatatat tgaaaacgag ggtgttagaa cagcagtatt
    16681 tcagaaaacc ctcgcgcaaa agcacgaggg tttgcaggag aggaagatta gccggtatta
    16741 cgcatacctg ccgcaatccc ggcaatagtg accattaacg cctgctcgac gcgaggatcc
    16801 ggttcatggc cttctttttc tgcctggcgg gagcggtgca gcaactcggc ctgcaatacg
    16861 ttcagcgggt cggtgtaaat attccgtagc tgaatagact ctgcaatcca cggcagatcg
    16921 gccatcaggt gagaatcgtt ggcaatcgcc agcaccactt tgatgtcttc ttcttgcaga
    16981 ttgcgtaact ctttacctaa cggccacagt gctttgtcga ccaggcgttg gtcatagtat
    17041 tctgccagcc acaggtctgc tttggcgaag accatctcca gcatgccgag acgcgtcgag
    17101 aagaatggcc aatcgcggca catggcttcc agctcgctct gtttgccatc ttcgaccact
    17161 ttttgcagcg ccgtacctgc acccagccag gcggggagca tcaggcggtt ttgcgtccag
    17221 gcaaaaatcc acggaatggc gcgcagcgac tcgacgccgc cggtcgggcg acgtttcgcc
    17281 ggacgtgaac ccaacggcag tttacccaat tcttgttccg gcgtagcgga gcggaagtaa
    17341 ggcacaaaat ctttgttttc acgtacgtag ccacggtaga gatcgcagga gatgactgac
    17401 agttcatcca taatgcgacg ccagctctct ttcggctcag gcggtggcag caggttggct
    17461 tccagaatcg ccccggtgta cagcgacagg ctgctgacgg tgatttctgg cagaccatat
    17521 ttaaagcgga tcatctcgcc ctgttcggtc acgcgcaggc cacctttcag gcttcctggc
    17581 ggttgtgaca gcagcgccgc atgggcaggt gcgccgccgc gaccaatgga accgccgcga
    17641 ccgtggaaca acgtcagctc aatacccgct ttttcgcagg ttttgattaa tgcatcctgt
    17701 gcctgatatt gcgcccagga agctgccatc acgcccgcat cttttgctga gtcggaatag
    17761 ccaatcatca ccatctgttt gccctgaatc aggccgcgat accagtcgat attcagcagc
    17821 tgggtcatga catcgttggc gttgttcagg tcatcgaggg tttcaaacag cggagcaacc
    17881 ggcatcgcaa acccgatacc cgcttctttc agcagcaggt ggacagccag cacgtcggac
    17941 ggcgttttcg ccatcgagat cacgtaggcg gcaatggagc cttgcggcgc ttcggcaatc
    18001 acctggcagg tatcgagcac ttcgcgcgtt tcggcgcttg gttgccagtt gcgcggtaga
    18061 agcggacgtt tggagttcag ttcgcggatc aggaacgcct gtttgtcggc ttctgaccag
    18121 ctctcgtagt cgccgatacc gaggtagcgg gtcagctcgc ccagcgcttc ggtatggcgg
    18181 gtgctctcct gacggatatc aatacgcacc agcggtacgc cgaaacattt cacgcggcgc
    18241 agggtgtcga gcagatcgcc attggcgata atacccatgc cacacgcctg aagtgactgg
    18301 tagcaagcgt agagcggttc ccacagttct tcgttttgtg tcagcagacc ttctggtttt
    18361 ggcagttctt cgcctttcag gcgcgcttcc agccatgcct gtgtcgccat caggcgagaa
    18421 cgcaggtttt tcatcagata gcgatacggt tctgcggcac cctcttcgcc aaccagtgcc
    18481 agcagttcag gggtggcttc aaccatcgac agttcagaaa ccagaacctg aatatctttc
    18541 aggaacaaat cggtggcttt ccagcggctg agtagcagga cgtggcgggt gatatcggca
    18601 gtgacgttcg gattgccgtc gcggtcaccg cccatccacg aggtaaaacg taccggaaca
    18661 aattcgacgg gcagtttgta gccgaggttc tcttccagtt gttcgttcag ttcgcgcagg
    18721 taattcggta cgccttgcca caggctgttt tctactacgg caaagcccca tttggcttca
    18781 tctaccgggc ttgggcgcag tttacggatt tcatcggtat gccatgactg ggcgatcaac
    18841 tggcgcaggc ggcgcatcag ctggttgtgt tcgtagtcgg cgatatcttt gttatcgagc
    18901 tgttttaaac aggcgttcac ttccaccatt ttgtggatca gtgtacgacg ggtaatttcg
    18961 gttgggtgag ccgtgaggac cagttccagc gacagcgatt ccactgcttt tttgatggtg
    19021 tcttcgctca gttccggctg gtttttcagt ttacgcaggg tgcgggcgat cacttccggg
    19081 ttgctggcag cttcgccttt cggcgaaatg ctgtggtatt gctcagcggt gttggccagg
    19141 ttcagaaact gactaaacgc acgcgcaacg ggcagcagct cgtcgttcga caaattttgt
    19201 aaggtggtga gcaactcctg gcggttagca tcattgccag cgcgtgaaga tttggacaac
    19261 ttacggatag tttctacgcg ttcaagaatg tgttctccca acgcatcctt gatggtttct
    19321 cccagcactt tgccgagcat actgacatta ctacgcaatg cggaatattg ttcgttcata
    19381 ttaccccaga caccccatct tatcgtttga tagccctgta tccttcacgt cgcattggcg
    19441 cgaatatgct cgggctttgc ttttcgtcgt cttttataaa gccacgtaaa agcggtgacg
    19501 tcaaatgctg cgaaatcgct tcagcaaacg aataaatagc aggaatttac gccattaaat
    19561 tcacgacgct ttaaataagc gtaacttatg ggaatgttaa aaaatcgcct cgagtaacac
    19621 caaaggtgta ggtcggataa gatgcgcaag catcgcatcc gacattgttt ggcctacgtt
    19681 ttaatgccag caaaaatggt gaatcacctg ggtgatcagt tcgcgggtgg gcttgataaa
    19741 cctcgtctcc agatattcat caggctgatg agcctgatta attgagccag gccccagaac
    19801 cagcgtcggg cataacgttt gaataaacgg cgcttcggta cagtaattca ccacttcggt
    19861 ctttgctccg agcaatttct caaccacttc aaccagttta tgattcggtg ggcattcata
    19921 gccagggatc ggcgggtgca gctcgtcgac cgtcagacga cccggccagc gttcgctcac
    19981 tggagccaat gcatcgttga gcaaaccatt aagttcattg agtgtcatgc ctggcagcgg
    20041 acgaatatcc atatgcaact cacaacaagc gcaaatacgg ttagaggcgt cgccgccgtg
    20101 aatatgcccg aggttgagtg tggggtatgg cacggtaaac gcttcatagt gatagcgttc
    20161 tttcaggtta tcgcgcaatt gcaaaatatg gccgatggcg tcgtgcatca gttcgatagc
    20221 gttaaccccg cgcgacggat cgctggagtg gcccgactga cccataattc gaatggcatt
    20281 tgagatgtgg cctttatgtg cgcgtaccgg ttgcagcgac gttggctcgc caatgatggc
    20341 gcaatccggg cgcagggcgg tagtttcggc aaaataacgc gctccggcca tactggtttc
    20401 ttcatcggca gtcgccagaa tgtagagcgg ttttgcaagc ttcgtgacgt cgacatcgcg
    20461 tagcgcatca aggataaacg caaaaaagcc tttcatgtcg gcggtgccta agccgtaaag
    20521 cttgccgtca tgctccgtca acgtaaatgg atcgcgcgtc cagcgtccgt catcaaatgg
    20581 caccgtatcg gtatgccccg ccagcaacaa accgccagcc ccctgtccgc aactggccag
    20641 catattaaat ttgttgcgag ttcctggcac agactgcact tccacattga agcctaaatc
    20701 cttaaaccag tccgccagca gagtgattaa atctgcattg ctttgatcga gtgcctcttc
    20761 cgtggcgctt attgaaggtg tggcaatcag agcacggtaa atctcgataa atggcggtaa
    20821 tttgtttttc attgttgaca cacctctggt catgatagta tcaatattca tgcagtattt
    20881 atgaataaaa atacactaac gttgagcgta ataaaaccca ccagccgtaa ggtgaatgtt
    20941 ttacgtttaa cctggcaacc agacataaga aggtgaatag ccccgatgtt gaatacgctg
    21001 attgtgggtg ccagtggcta cgctggcgca gagctagtga cctatgtaaa tcgccatccg
    21061 catatgaaca taaccgcttt gactgtttca gcgcaaagca atgatgcggg aaagttaatc
    21121 tccgatttgc atccgcagct aaaaggcatc gttgatctgc cgttgcagcc gatgtcggat
    21181 atcggcgagt ttagcccagg ggtggacgtg gtgtttctcg ccaccgccca tgaagtcagc
    21241 cacgatttag cgccgcaatt tcttgaagca ggctgtgtgg tgttcgacct ttccggcgcg
    21301 tttcgtgtta acgatgtcgc cttctatgaa aaatattacg gctttaccca tcaatacccg
    21361 gaactgctgg aacaggcagc ctacggtctg gcggagtggt gcggtaataa attaaaagaa
    21421 gcgaacttga ttgccgtgcc aggctgttat ccgacggcag cacagctggc gctgaaaccg
    21481 ttgattgatg ccgatcttct tgacctcaat cagtggccgg tgatcaacgc caccagcggc
    21541 gtgagcggtg caggacgtaa ggcagcgatt tcaaatagtt tttgtgaagt tagcctgcaa
    21601 ccgtatggcg tctttaccca tcgccatcaa ccagagatcg ccacacacct cggtgctgac
    21661 gttatcttca ccccgcatct gggcaatttc ccgcgcggca ttctcgaaac cattacctgc
    21721 cgcctgaaac cgggtgtgag ccaggtgcaa gtggcgcaag cgttacagca ggcgtatgcc
    21781 cataaaccac tggtgcggct gtatgacaaa ggcgttccgg cgctgaaaaa tgtcgttgga
    21841 ctgccattct gcgatatcgg ttttgccgtt cagggcgagc atctgattat tgtggcgacc
    21901 gaagacaact tactgaaagg cgcggcggca caagcggtgc agtgcgccaa tattcgtttc
    21961 ggctatgcgg aaacgcagtc tcttatttaa gggtgcaatg atgaatccat taattatcaa
    22021 actgggtggc gtactgctgg atagtgaaga ggcgctggaa cgtctgttta gcgcactggt
    22081 gaattatcgc gagtcacatc agcgtccgct ggtgattgtg cacggcggcg gttgtgtggt
    22141 ggatgagctg atgaaagggc tgaatctgcc ggtgaaaaag aaaaatggcc tgcgggtgac
    22201 gcctgcggat cagatagaca ttatcaccgg agcactggcg ggaacggcaa ataaaaccct
    22261 gctggcatgg gcgaagaaac atcagattgc ggccgtaggt ttgtttctcg gtgacggcga
    22321 cagcatcaaa gtgacccagc ttgatgaaga gttaggccat gttggactgg cgcagccagg
    22381 ttcgcctaag cttatcaaca gtttgctgga gaacggttat ctgccggtgg tcagctccat
    22441 tggcgtaaca gacgaagggc aactgatgaa cgtcaatgcc gaccaggcgg cgacggcgct
    22501 ggcggcaacg ctgggcgcgg atctgatttt gctctctgac gtcagcggca ttctcgacgg
    22561 caaagggcaa cgtattgccg aaatgaccgc cgcgaaagca gaacaactga ttgagcaggg
    22621 cattattact gacggcatga ttgtgaaagt gaacgcggcg ctggatgctg cgcgcacgtt
    22681 gggccgtccg gtagatatcg cctcctggcg tcatgcggaa cagcttccgg cactgtttaa
    22741 cggtatgccg atggggacgc ggattttagc ttaagttttg ttggccggag gcgcagcttt
    22801 ccggcattga atttcaaaat aaggaaacag agttatggca ctttggggcg ggcgttttac
    22861 ccaggcagca gatcaacggt tcaaacaatt caacgactca ctgcgctttg attaccgtct
    22921 ggcggagcag gatattgttg gctctgtggc ctggtccaaa gccctggtaa cggtcggcgt
    22981 gttaaccgca gaagagcagg cgcaactgga agaggcgctg aacgtgctgc tggaagatgt
    23041 tcgcgccagg ccacaacaaa tccttgaaag cgacgccgaa gatatccata gctgggtgga
    23101 aggcaaactg atcgacaaag tgggtcagtt aggcaaaaaa ttgcataccg ggcgtagccg
    23161 taatgatcag gtagcaactg acctgaaact gtggtgcaaa gataccgtta gcgagttgct
    23221 gacggctaac cggcagttac aatccgctct ggtcgagacc gcgcagaaca atcaggacgc
    23281 ggtgatgccg ggttacaccc acctgcaacg cgcccagccg gtgacgttcg cgcactggtg
    23341 cctggcctac gttgagatgc tggcgcgtga tgaaagccgt ttgcaggatg cgcttaagcg
    23401 tctggatgtc agcccgctag gctgtggcgc actggcagga acggcctatg aaatcgaccg
    23461 tgaacagtta gcaggttggc tgggctttgc ttcggcgacc cgtaacagtc tcgacagcgt
    23521 ttctgaccgt gaccatgtgt tggaactgct ttctgctgcc gctatcggca tggtgcatct
    23581 gtcgcgtttt gctgaagatc tgattttctt taacaccggc gaagcggggt ttgtggagct
    23641 ttctgaccgt gtgacttccg gttcatcatt aatgccgcag aagaaaaacc cggatgcgct
    23701 ggagctgatt cgcggtaaat gcggtcgggt gcagggggcg ttaaccggca tgatgatgac
    23761 gctgaaaggt ttgccgctgg cttacaacaa agatatgcag gaagacaaag aaggtctgtt
    23821 cgacgcgctc gatacctggc tggactgcct gcatatggcg gcgctggtgc tggacggcat
    23881 tcaggtgaaa cgtccgcgtt gccaggaagc ggcgcagcag ggttactcca acgctactga
    23941 actggcggat tatctggtgg cgaaaggcgt accgttccgc gaggcgcacc atattgtggg
    24001 tgaagcggtg gtggaagcca ttcgtcaggg caaaccgctg gaagatctgc cactcagtga
    24061 gttgcagaaa ttcagcctgg tgattggcga agatgtctat ccgattctgt cgctgcaatc
    24121 gtgcctcgac aagcgtgcgg caaaaggcgg tgtctcaccg caacaggtgg cgcaggcgat
    24181 tgcttttgcg caggcgcggt tggggtaaga acatttatat gtataaattt gagcctggct
    24241 tatcgctggg cttttttatg gcaaaaaaaa gcggatcctg gagatccgca aaagttcacg
    24301 ttggctttag ttattcgagc tgagaaactc tcgaaacggg ctgtgacttc aagggttaaa
    24361 agaggtgccg ctccgtttct gtgagcaatt atcagtcaga atgcttgata gggataatcg
    24421 ttcattgcta ttctacctat cgccatgaac tatcgtggcg atggaggatg gataatgaat
    24481 attcgtgatc ttgagtacct ggtggcattg gctgaacacc gccattttcg gcgtgcggca
    24541 gattcctgcc acgttagcca gccgacgctt agcgggcaaa ttcgtaagct ggaagatgag
    24601 ctgggcgtga tgttgctgga gcggaccagc cgtaaagtgt tattcaccca ggcgggaatg
    24661 ctgctggtgg atcaggcgcg taccgtgctg cgtgaggtga aagtccttaa agagatggca
    24721 agccagcagg gcgagacgat gtccggaccg ctgcatattg gtttgattcc cacggttgga
    24781 ccgtacctgc taccgcatat tatcccgatg ctgcaccaga cctttccaaa gctggaaatg
    24841 tatctgcatg aagcacagac ccaccagtta ctggcgcaac tggacagcgg caaactcgat
    24901 tgcgtgatcc tcgcgctggt gaaagagagc gaagcattca ttgaagtgcc gttgtttgat
    24961 gagccaatgt tgctggctat ctatgaagac cacccgtggg cgaaccgcga atgcgtaccg
    25021 atggccgatc tggcagggga aaaactgctg atgctggaag atggtcactg tttgcgcgat
    25081 caggcaatgg gtttctgttt tgaagccggg gcggatgaag atacacactt ccgcgcgacc
    25141 agcctggaaa ctctgcgcaa catggtggcg gcaggtagcg ggatcacttt actgcctgcg
    25201 ctggctgtgc cgccggaacg caaacgcgat ggggttgttt atctgccgtg cattaagccg
    25261 gaaccacgcc gcactattgg cctggtttat cgtcctggct caccgctgcg cagccgctat
    25321 gagcagctgg cagaggccat ccgcgcaaga atggatggcc atttcgataa agtgttaaaa
    25381 caggcggttt aagccattta gcgcagctac ccgataggct tccgccatcg tcgggtagtt
    25441 aaaggtggtg ttgacgaagt actcaatagt gttgccgcca cctttctgtt ccataatcgc
    25501 ctgaccgata tgaataattt cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc
    25561 ttttgtttcc cgatggaaca aaattttcag cgtgcccacg ttcatgccga cgatttgtgc
    25621 acgtgccaga tgtttaaact gggcgcggcc tacttcatat ggcactttca tcgcggtcag
    25681 ctgctgttcg gttttgccta cagagctgat ttccgggatg gtgtaaatgc cggtagggat
    25741 atcttcaatc agatgtgcgg tggcttcacc tttcaccagc gcctgcgcgg caatgcgccc
    25801 ctggtcatag gctgccgacg ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac
    25861 gtgcggctgt gcggtctgat acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc
    25921 cagcccaatg ttctgtaacg ccagcgaatc ggtattaccg gtgcgaccgt tggcatagag
    25981 cagacagtca gctttcagtt ttttacccga cttcagatgc atgatcacac cgtcatcaca
    26041 gccttcgatc ttctcgtact cttcgttgtg acgaatcact acgccactgt tccagaagtg
    26101 ataggagaga gaatctgaca tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt
    26161 gatcagatcc acttttacat ccataccgcg gaagatcgac gcatattcac agccgatcac
    26221 tccagcaccg taaataagta catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct
    26281 gtcataaatg cgtggatggg tgaaatcaac atctgttgga tgatatggac gagaaccgca
    26341 ggcaataaca aatttttcag cagttagtgt ttcgacgctg ccgtccgggc aatccagcgc
    26401 caacgtatgc tcatcaacaa agcgagcgtt cccctgcaat atttcacagt gattacgttc
    26461 gtaaaatccc tgacgcatgc gcgtttgttg attaatcacg ttatcggcat ggttaaggat
    26521 atcggcaaaa gaagagcgga gcagtcggga atggtcgctg tatagtgggt tttgattgaa
    26581 ttctataatg cggctgacgg cgtgacggag agctttcgac gggatggtgc cccagtgggt
    26641 gcaaccgccg ccaacatttt gataacgctc gataactgcg acgcgcgcac cttgcttaac
    26701 caggcccatc gcagcgcctt cgccgccggg gccggaacct attactatgg catcgtaatc
    26761 gtaggaatgt ggcatggtag ggcttacctg ttcttataca taaaagcaac agaatggtaa
    26821 cattttatcg cgggtaagcc aattgatccc cgtcatttat ctggctatat cgtgagcggc
    26881 ctttgctttg tcttcctcct tcttatttta tttattcact attaatcgct ttttcttat
//
LOCUS       DACADF010000062        26473 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.062, whole genome shotgun sequence.
ACCESSION   DACADF010000062 DACADF010000000
VERSION     DACADF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.062"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            21..1457
                     /gene="gspA"
                     /locus_tag="ISB30_002386"
     CDS             21..1457
                     /gene="gspA"
                     /locus_tag="ISB30_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HAP1990013.1"
                     /translation="MREKHQTWRLCYLLGEAGSGKTRLAQQLQKDKHRRVITLSLVVS
                     WQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLIIIENAHLNHRR
                     ILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEHELKASILEGQN
                     IDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTCVFTAKQWRMIYRVL
                     GDNRPRKMQLAVVMSGTIIALTCGWLLLPSFTAPLPVPAWLIPVTPVVKQDMTKDIAH
                     VVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTLVDQNLPWIASL
                     KVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLWKMSPEGESTIT
                     RDSSEEEILWLETMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLKTDGVVGFSTLV
                     HLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1459..1878
                     /gene="gspB"
                     /locus_tag="ISB30_002387"
     CDS             1459..1878
                     /gene="gspB"
                     /locus_tag="ISB30_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HAP1990014.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEDNTVEESDEKAGLRERVKNALNELER"
     gene            2116..2427
                     /gene="rpsJ"
                     /locus_tag="ISB30_002388"
     CDS             2116..2427
                     /gene="rpsJ"
                     /locus_tag="ISB30_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP1990015.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2460..3089
                     /gene="rplC"
                     /locus_tag="ISB30_002389"
     CDS             2460..3089
                     /gene="rplC"
                     /locus_tag="ISB30_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP1990016.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3100..3705
                     /gene="rplD"
                     /locus_tag="ISB30_002390"
     CDS             3100..3705
                     /gene="rplD"
                     /locus_tag="ISB30_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP1990017.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3702..4004
                     /gene="rplW"
                     /locus_tag="ISB30_002391"
     CDS             3702..4004
                     /gene="rplW"
                     /locus_tag="ISB30_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP1990018.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4022..4843
                     /gene="rplB"
                     /locus_tag="ISB30_002392"
     CDS             4022..4843
                     /gene="rplB"
                     /locus_tag="ISB30_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP1990019.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4860..5138
                     /gene="rpsS"
                     /locus_tag="ISB30_002393"
     CDS             4860..5138
                     /gene="rpsS"
                     /locus_tag="ISB30_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP1990020.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5153..5485
                     /gene="rplV"
                     /locus_tag="ISB30_002394"
     CDS             5153..5485
                     /gene="rplV"
                     /locus_tag="ISB30_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP1990021.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5503..6204
                     /gene="rpsC"
                     /locus_tag="ISB30_002395"
     CDS             5503..6204
                     /gene="rpsC"
                     /locus_tag="ISB30_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP1990022.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6217..6627
                     /gene="rplP"
                     /locus_tag="ISB30_002396"
     CDS             6217..6627
                     /gene="rplP"
                     /locus_tag="ISB30_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP1990023.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6627..6818
                     /gene="rpmC"
                     /locus_tag="ISB30_002397"
     CDS             6627..6818
                     /gene="rpmC"
                     /locus_tag="ISB30_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP1990024.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6818..7072
                     /gene="rpsQ"
                     /locus_tag="ISB30_002398"
     CDS             6818..7072
                     /gene="rpsQ"
                     /locus_tag="ISB30_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP1990025.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7237..7608
                     /gene="rplN"
                     /locus_tag="ISB30_002399"
     CDS             7237..7608
                     /gene="rplN"
                     /locus_tag="ISB30_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP1990026.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7619..7933
                     /gene="rplX"
                     /locus_tag="ISB30_002400"
     CDS             7619..7933
                     /gene="rplX"
                     /locus_tag="ISB30_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP1990027.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7948..8487
                     /gene="rplE"
                     /locus_tag="ISB30_002401"
     CDS             7948..8487
                     /gene="rplE"
                     /locus_tag="ISB30_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP1990028.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8502..8807
                     /gene="rpsN"
                     /locus_tag="ISB30_002402"
     CDS             8502..8807
                     /gene="rpsN"
                     /locus_tag="ISB30_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP1990029.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8841..9233
                     /gene="rpsH"
                     /locus_tag="ISB30_002403"
     CDS             8841..9233
                     /gene="rpsH"
                     /locus_tag="ISB30_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP1990030.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9246..9779
                     /gene="rplF"
                     /locus_tag="ISB30_002404"
     CDS             9246..9779
                     /gene="rplF"
                     /locus_tag="ISB30_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP1990031.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9789..10142
                     /gene="rplR"
                     /locus_tag="ISB30_002405"
     CDS             9789..10142
                     /gene="rplR"
                     /locus_tag="ISB30_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP1990032.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10157..10660
                     /gene="rpsE"
                     /locus_tag="ISB30_002406"
     CDS             10157..10660
                     /gene="rpsE"
                     /locus_tag="ISB30_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP1990033.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10664..10843
                     /gene="rpmD"
                     /locus_tag="ISB30_002407"
     CDS             10664..10843
                     /gene="rpmD"
                     /locus_tag="ISB30_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP1990034.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10847..11281
                     /gene="rplO"
                     /locus_tag="ISB30_002408"
     CDS             10847..11281
                     /gene="rplO"
                     /locus_tag="ISB30_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP1990035.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11289..12620
                     /gene="secY"
                     /locus_tag="ISB30_002409"
     CDS             11289..12620
                     /gene="secY"
                     /locus_tag="ISB30_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP1990036.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12652..12768
                     /gene="rpmJ"
                     /locus_tag="ISB30_002410"
     CDS             12652..12768
                     /gene="rpmJ"
                     /locus_tag="ISB30_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP1990037.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12915..13271
                     /gene="rpsM"
                     /locus_tag="ISB30_002411"
     CDS             12915..13271
                     /gene="rpsM"
                     /locus_tag="ISB30_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP1990038.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13288..13677
                     /gene="rpsK"
                     /locus_tag="ISB30_002412"
     CDS             13288..13677
                     /gene="rpsK"
                     /locus_tag="ISB30_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP1990039.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADVVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13711..14331
                     /gene="rpsD"
                     /locus_tag="ISB30_002413"
     CDS             13711..14331
                     /gene="rpsD"
                     /locus_tag="ISB30_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP1990040.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14357..15346
                     /gene="rpoA"
                     /locus_tag="ISB30_002414"
     CDS             14357..15346
                     /gene="rpoA"
                     /locus_tag="ISB30_002414"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP1990041.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15387..15770
                     /gene="rplQ"
                     /locus_tag="ISB30_002415"
     CDS             15387..15770
                     /gene="rplQ"
                     /locus_tag="ISB30_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP1990042.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15877..16245
                     /locus_tag="ISB30_002416"
     CDS             15877..16245
                     /locus_tag="ISB30_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HAP1990043.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEHRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16256..16681
                     /gene="zntR"
                     /locus_tag="ISB30_002417"
     CDS             16256..16681
                     /gene="zntR"
                     /locus_tag="ISB30_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HAP1990044.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16737..16955
                     /locus_tag="ISB30_002418"
     CDS             16737..16955
                     /locus_tag="ISB30_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HAP1990045.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(16952..17365)
                     /gene="mscL"
                     /locus_tag="ISB30_002419"
     CDS             complement(16952..17365)
                     /gene="mscL"
                     /locus_tag="ISB30_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP1990046.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17495..18871)
                     /gene="trkA"
                     /locus_tag="ISB30_002420"
     CDS             complement(17495..18871)
                     /gene="trkA"
                     /locus_tag="ISB30_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAP1990047.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18893..20182)
                     /gene="rsmB"
                     /locus_tag="ISB30_002421"
     CDS             complement(18893..20182)
                     /gene="rsmB"
                     /locus_tag="ISB30_002421"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP1990048.1"
                     /translation="MKKQRNLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVQLETPAPVH
                     ALPGFEEGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRNPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20234..21181)
                     /gene="fmt"
                     /locus_tag="ISB30_002422"
     CDS             complement(20234..21181)
                     /gene="fmt"
                     /locus_tag="ISB30_002422"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP1990049.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            complement(21196..21705)
                     /gene="def"
                     /locus_tag="ISB30_002423"
     CDS             complement(21196..21705)
                     /gene="def"
                     /locus_tag="ISB30_002423"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP1990050.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21835..22959
                     /gene="dprA"
                     /locus_tag="ISB30_002424"
     CDS             21835..22959
                     /gene="dprA"
                     /locus_tag="ISB30_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP1990051.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22931..23404
                     /locus_tag="ISB30_002425"
     CDS             22931..23404
                     /locus_tag="ISB30_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAP1990052.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLSLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23433..23975
                     /locus_tag="ISB30_002426"
     CDS             23433..23975
                     /locus_tag="ISB30_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990053.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACGYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            23980..24552
                     /gene="tsaC"
                     /locus_tag="ISB30_002427"
     CDS             23980..24552
                     /gene="tsaC"
                     /locus_tag="ISB30_002427"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAP1990054.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKRRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24557..25375
                     /gene="aroE"
                     /locus_tag="ISB30_002428"
     CDS             24557..25375
                     /gene="aroE"
                     /locus_tag="ISB30_002428"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP1990055.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLTPINDFI
                     NTLNTFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIHALGMHELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25372..25629
                     /locus_tag="ISB30_002429"
     CDS             25372..25629
                     /locus_tag="ISB30_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAP1990056.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLESFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25605..26159)
                     /locus_tag="ISB30_002430"
     CDS             complement(25605..26159)
                     /locus_tag="ISB30_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP1990057.1"
                     /translation="MSEVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPAGNPLTIGEDVTIGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYMDQGNHTQP"
BASE COUNT         6755 a         6238 c         6910 g         6570 t
ORIGIN      
        1 gagaagtcat tctttcctgg ttgcgtgaga agcatcagac ctggcgtctg tgctatttgt
       61 tgggtgaggc tggaagtgga aaaacccggc tggcgcagca actacaaaaa gataaacatc
      121 gccgtgtgat tactttaagc ctcgttgttt cctggcaagg taaggccgca tggatcgtta
      181 ccgacgataa tgcggctgaa cagggctgcc gtgacagcgc ctggacgcga gatgagatgg
      241 cgggtcaatt actgcatgca cttcatcgaa ctgatagtcg ctgccctctt attattattg
      301 aaaatgctca cctgaatcac cgccgcatac ttgatgatct ccaacgcgcg atatctctta
      361 ttcctgacgg tcaatttttg ctgatcggca gacccgatcg aaaagtcgaa cgtgatttta
      421 aaaaacaggg cattgaactt gtctcgacag gtcgtctgac ggagcacgag cttaaggcaa
      481 gcatccttga aggtcagaat attgaccagc ccgatctcct tttaaccgcc agagttctga
      541 aacggatagc tttattatgt cggggagatc gcagaaagct ggcgcttgcc ggcgaaacaa
      601 ttagcttatt acagcaagcc gagcagacct gcgttttcac tgcaaaacaa tggcgaatga
      661 tttaccgcgt attaggtgat aaccgccccc ggaaaatgca gcttgccgtg gtgatgagtg
      721 gaaccattat cgcacttacc tgtggttggc tactgctgcc ctctttcaca gctccgttac
      781 ccgttcctgc gtggctgata cccgtaacgc cagtagttaa acaagatatg actaaggata
      841 ttgcccatgt ggtgatgcgc gatagtgagg cgctaagcgt gttatacggc gtatgggggt
      901 acgaggtccc agccgattcg gcctggtgcg atcaggcagt aagagcgggg ctggtatgta
      961 aaagtgggaa tgcttcttta caaacattgg tagatcagaa tttgccctgg atcgcatcgc
     1021 ttaaagtcgg ggacaaaaaa ttgcctgttg tggtcgttcg cgtcggtgat gctaccgttg
     1081 atgtactcgt agggcagcaa acctggacgc tgacacataa atggtttgag ttggtatgga
     1141 cgggtgatta tcttctgttg tggaaaatgt cgccggaagg agagagtacc attacgcgtg
     1201 atagcagcga ggaagagatt ctctggctgg aaacgatgtt aaaccgcgca ttgcatattt
     1261 cgacagaatc ttcggcagag tggcgtccct tgttggtaga aaaaataaag caatttcaaa
     1321 aaagccatca cttgaaaact gacggtgttg ttggcttttc aacgttagtg catctctggc
     1381 aggtggcagg ggagagtgcc tacttatatc gggatgaagc aaatatttcc ccggaaacaa
     1441 cagtgaaggg gaaatgacat gtttgaattc tatatcgccg cgcgtgagca gaaagaaaca
     1501 ggtcaccctg gtatcttctc ccgacaaaaa cacagcacca taatatatgt catttgttta
     1561 ttgctgattt gcctgtggtt tgcaggaatg gtgttggttg gtgggtatgc ccggcagcta
     1621 tgggcacttt ggatagtaaa agcagaagtc actgtagatg ctgaaacgcc cgcttttaaa
     1681 caatcaacac aacactattt tttcaaaaaa cagccactcc ccgtcgtcga atccgtcgag
     1741 gaggaagatg acccaggagt cgcggttgag aatgccccct cttcttctga agacgaagac
     1801 aatactgtag aagaaagcga cgagaaagcc ggtttaagag agcgtgtcaa aaatgcgctg
     1861 aacgaacttg agagataacc caaaggctgt ttactttact agcaatacgc ttgcgttcgg
     1921 tggttaagta tgtataatgc gcgggcttgt cgtagttgac agcaggttca atctgaacct
     1981 cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg taagaacggt
     2041 tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt ataaaataat
     2101 tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc gtttgatcat
     2161 cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ctaagcgcac tggtgcgcag
     2221 gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct gatctccccg
     2281 cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg tctggttgac
     2341 atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct ggctgccggt
     2401 gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag gttgaaacaa
     2461 tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa gacggcgttt
     2521 ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt aaagacctgg
     2581 ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct aaccgtgtga
     2641 ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt ggtctgtggg
     2701 aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc gttgaactgt
     2761 ttgctgacgt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt ttcgcaggta
     2821 ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc ttgtctcacc
     2881 gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa ggcaagaaaa
     2941 tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta gtacgcgttg
     3001 acgctgagcg caacctgctg ctggttaaag gcgctgtccc gggtgcaacc ggtagcgacc
     3061 tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt attgaaagac
     3121 gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa cgaagcgctg
     3181 gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg tgctcagaag
     3241 actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg caccggccgt
     3301 gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac ctttgctgct
     3361 cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc gctgaaaagc
     3421 atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt ctctgtagaa
     3481 gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga agatgtgctg
     3541 atcatcaccg gtgagctgga cgagaacctg ttcctggctg cgcgcaacct gcacaaggtt
     3601 gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga caaagtcgta
     3661 atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt gaagaacgtc
     3721 tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg atggaaaaat
     3781 ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc aaagctgctg
     3841 tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt aaagggaaag
     3901 ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct tacgtcaccc
     3961 tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga ggagtaatac
     4021 aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag ttaaagtggt
     4081 taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa acagcaaatc
     4141 cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg gccacaagca
     4201 ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag ttgttgaacg
     4261 tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca aagacggtga
     4321 acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc agtctggcgt
     4381 tgatgctgca atcaaaccag gtaacaccct gccgatgcgc aacatcccgg ttggttctac
     4441 tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt ccgctggtac
     4501 ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc gttctggtga
     4561 aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca atgctgagca
     4621 tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc gtccgaccgt
     4681 tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag gtcgtaactt
     4741 tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga cccgcagcaa
     4801 caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga ggataagcca
     4861 tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag aaggtagaga
     4921 aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt tcaacgatct
     4981 ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac gttccggtat
     5041 ttgtaaccga cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact cgtacttatc
     5101 gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag agatggaaac
     5161 tatcgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg ttgctgacct
     5221 gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca acaagaaagc
     5281 ggctgtactg gtcaagaaag ttctggaatc tgccattgct aacgctgaac acaacgatgg
     5341 cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc cgagcatgaa
     5401 gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca ccagccacat
     5461 cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca gaaagtacat
     5521 cctaatggta ttcgcctggg tattgtaaaa ccatggaact ctacctggtt tgcgaacacc
     5581 aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct gactaaggaa
     5641 ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag catccgtgta
     5701 accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga cgtagaaaaa
     5761 ctgcgtaagg tcgtagcgga catcgctggc gttcctgcac agatcaacat cgccgaagtt
     5821 cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca gctggaacgt
     5881 cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg tctgggcgct
     5941 aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc acgtaccgaa
     6001 tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga ctacaacacc
     6061 tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt caaaggcgag
     6121 atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca gcctaaaaag
     6181 cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa gcgtacaaaa
     6241 ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga tgttagcttc
     6301 ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcacgtca gatcgaagca
     6361 gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat ccgtgtgttc
     6421 ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg taaaggtaac
     6481 gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat ggacggtgtt
     6541 ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc gattaaaacc
     6601 acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag aagagcgttg
     6661 aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg cgtatgcagg
     6721 ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc gatgtcgcac
     6781 gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa tccgtactct
     6841 gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta tcgaacgttt
     6901 tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc acgtacatga
     6961 cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc gtccgctgtc
     7021 caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt aatacagtac
     7081 actctctcga tacgaataaa cggctcagaa atgagccgtt tattttttct acccatatcc
     7141 ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac ctgattttcg
     7201 ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca gactatgctg
     7261 aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct gggtggctcg
     7321 caccgtcgct acgcaggcgt aggcgatatc atcaagatca ccatcaaaga agcaattccg
     7381 cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac caagaagggt
     7441 gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt tcttctgaac
     7501 aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg tgagcttcgt
     7561 agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg agcgaatcat
     7621 ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag ataaaggtaa
     7681 acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag gtatcaatct
     7741 ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca tcgttgaaaa
     7801 agaagccgct attcaggttt ccaacgtagc aatcttcaat gcgacaaccg gcaaggctga
     7861 ccgtgtaggc tttagattcg aagacggtaa aaaagtccgt ttcttcaagt ctaacagcga
     7921 aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta caaagacgaa
     7981 gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt ccctcgggtc
     8041 gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa actgctggat
     8101 aacgcagcag cagacctggc agcaatctcc ggtcaaaaac cgctgatcac caaagcacgc
     8161 aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa agtaactctg
     8221 cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt acctcgtatc
     8281 cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta cagcatgggt
     8341 gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg cgttcgtggt
     8401 ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc tctgctggct
     8461 gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag caatcaatga
     8521 aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa cgcgctgaac
     8581 tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac gctgttctca
     8641 agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc tgccgtcaaa
     8701 caggtcgtcc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag gtccgtgaag
     8761 ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg tcaccaattg
     8821 aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg ctgacccgta
     8881 tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc aagctgaaag
     8941 tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa gttgaaggcg
     9001 acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct gttgtagaaa
     9061 gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat gagctgccga
     9121 aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt atgactgatc
     9181 gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc taatcggagg
     9241 aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt gacgtaaaaa
     9301 tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt actctcaacg
     9361 atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt gatggttacg
     9421 cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg gttatcggtg
     9481 ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt gcagcggtta
     9541 aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat cagctgcctg
     9601 cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc gctgataagc
     9661 aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag ccttacaaag
     9721 gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag aagaagtaag
     9781 gtaacactat ggataagaaa tctgctcgta tccgtcgtgc gacccgcgca cgccgcaagc
     9841 tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac atttacgcac
     9901 aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta gaaaaagcta
     9961 tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg ggtaaagctg
    10021 tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt tccgggttcc
    10081 aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc cttcagttct
    10141 aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc aggaaaagct
    10201 gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct ccttcacagc
    10261 tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta aagcgcgtga
    10321 agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga ttaacgtcgc
    10381 gctgaataac ggcactctgc aacaccctgt taaaggtgtt cacacgggtt ctcgcgtatt
    10441 catgcagccg gcttccgagg gtaccggtat catcgccggt ggtgcaatgc gcgccgttct
    10501 ggaagtcgct ggggttcata acgttctggc taaagcgtat ggttccacca acccgattaa
    10561 cgtggttcgt gcaactatcg atggcctgga aaatatgaat tctccagaaa tggtcgctgc
    10621 caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa agactattaa
    10681 aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa cgctgcttgg
    10741 cctgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg ctattcgcgg
    10801 tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc gtttaaatac
    10861 tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg gtatcggttc
    10921 tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aaatctcgtt ctggcggtgg
    10981 cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc cgaaattcgg
    11041 cttcacttct cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc tggctaaagt
    11101 agaaggcggt gttgtagacc tgaacacgct gaaagcggct aacattatcg gtatccagat
    11161 cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg ttcgtggcct
    11221 gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa tcgaggaata
    11281 agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt ggcttaggcg
    11341 agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt attggctctt
    11401 ttattccgat ccctggtatt gatgccgctg tacttgccaa actgcttgag caacagcgag
    11461 gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt gcttctatct
    11521 ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagcta ctgacggtgg
    11581 ttcatccaac gttggcagaa attaagaaag aaggggagtc tggtcgtcgt aagatcagcc
    11641 agtacacccg ctacggtact ctggtgctgg caatattcca gtcgatcggt attgctactg
    11701 gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt gcattctact
    11761 tcaccgctgt tgtaagtctg gtcacaggaa ccatgttcct gatgtggttg ggcgaacaga
    11821 tcactgaacg aggtatcggc aacggtattt caatcattat cttcgccggt attgtcgcgg
    11881 gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac ctgcacttcc
    11941 tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt gtatttgttg
    12001 agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt cgtcgtgtct
    12061 atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta atcccggcaa
    12121 tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc gggggcggta
    12181 ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa ccgctttatg
    12241 tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg ttggttttca
    12301 acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca ggaattcgtc
    12361 cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc ttggttggtg
    12421 cgctgtacat tacctttatc tgcctgatcc cggagttcat gcgtgatgca atgaaagtac
    12481 cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg gactttatgg
    12541 ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag aaggcgaacc
    12601 tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa aatgaaagtt
    12661 cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga tggtgtcatc
    12721 cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt ttttcgcata
    12781 tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt tgtatgtctg
    12841 tgcgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta catattaaat
    12901 agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat aagcatgccg
    12961 taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc atcctggctg
    13021 cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa atcgacacgc
    13081 tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa atcagcatga
    13141 gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt cgtggtctcc
    13201 cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg cgcaaaccga
    13261 tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc acgtaaacgt
    13321 gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa caacaccatc
    13381 gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg tggttccggt
    13441 ttccgtggtt ctcgcaaatc cactccgttt gcagcacagg ttgcagcaga gcgttgcgct
    13501 gacgtcgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg tccgggtcca
    13561 ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac taacattact
    13621 gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg cgtataacgc
    13681 ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc taagctcaag
    13741 ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc gatcgatacc
    13801 aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg tctgtctgac
    13861 tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt gctggagcgt
    13921 cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg tgaaaacctg
    13981 ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt cggtgccact
    14041 cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg tcgtgttgtt
    14101 aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga gaaagcgaag
    14161 aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa gccaacctgg
    14221 ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga gcgttctgat
    14281 ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta aagcttagta
    14341 ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc gcctggttga
    14401 tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag agcgtggctt
    14461 tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc cgggttgcgc
    14521 ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag aaggcgttca
    14581 ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag ttcagggcaa
    14641 agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg cagccgatat
    14701 cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc acctgaccga
    14761 tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt atgtgccggc
    14821 ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc tggtcgacgc
    14881 atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg tagaacagcg
    14941 taccgacctg gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg atcctgaaga
    15001 ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg ttgacttacg
    15061 tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga tcctgctgcg
    15121 ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag cagaagctat
    15181 ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa cgcctaacct
    15241 tggtaaaaaa tctcttactg agattaaaga cgtgctggct tcccgtggac tgtctctggg
    15301 catgcgcctg gaaaactggc caccggcaag catcgctgac gagtaaccgg atcacaggtt
    15361 aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt caactgaacc
    15421 gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg gttcgtcatg
    15481 aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt gagccgctga
    15541 ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc cgtactcgtg
    15601 ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg agccgtgccg
    15661 gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaatgcg ccgatggctt
    15721 acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa tctgaagcaa
    15781 cgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gcatccccac ttatctacaa
    15841 tagctgtact ctttttgttc atcccctgga gtatttatgt ggttacttga ccagtgggca
    15901 gagcgtcata tagcagaagc gcaagcgaaa ggtgagtttg ataacctggt aggtagcggc
    15961 gaaccattga tactggatga tgattctcat gtgccaccgg aattacgtgc ggggtatcgc
    16021 ttgctgaaga atgctggttg cttaccgcca gaacttgagc ataggaggga agcaatacag
    16081 cttctggata ttctcaaagg tatccgtcat gatgatccgc aatatcaaga ggttagtcgt
    16141 cgattgtcat tactggaatt gaagctgcga caagctggat tgagtaccga ttttttacgc
    16201 ggcgattatg ctgacaagtt gttgaacaaa atcaacgata actagtggag tatgtatgta
    16261 tcgcattggt gagctggcaa aaatggcgga agtaacaccc gacacgattc gttattacga
    16321 aaagcagcag atgatggagc atgaagtacg caccgaaggt gggtttcgcc tgtataccga
    16381 aagcgatctc cagcgattga aatttatccg ccatgccaga caactaggtt tcagtctgga
    16441 gtcgatccgc gagttgctgt cgatccgtat cgatcctgaa caccatacct gtcaggagtc
    16501 aaaaggcatt gtgcaggaaa gattgcagga agtcgaagct cgtatagccg agttgcagag
    16561 tatgcagcgt tccttgcaac gccttaatga tacctgttgt gggaccgccc atagcagtgt
    16621 ttattgctcg attcttgaag ctcttgaaca aggggcgagt ggcgttaaga gtggctgttg
    16681 attttttgca ctggcaggat tacactcgcg ccgttaaata accaattgga gtttttatga
    16741 gccgatatca gcatactaaa gggcagataa aggataatgc gatagaagca ttactacatg
    16801 atcccttatt ccgacagcga gtagagaaaa ataagaaagg gaaaggcagt tacatgcgaa
    16861 aaggaaaaca tggcaatcgg gggaactggg aggccagtgg caagaaagta aatcactttt
    16921 ttaccactgg ccttctgctt tcagacgctt gttaagagcg gttattctgc tctttcagca
    16981 aatcacgaat ttctgtcagt aatacttctt ctttagttgg tgcaggtgtt gctgctgctg
    17041 gttcttcttt tttccgattc agtttgttga ttagcttaat cgccataaag atggcaaagg
    17101 ccactatcag aaaatcaaag acgttttgaa tgaagacacc gtaatgcatc acaacagcag
    17161 ggatatcccc ttgggcctcg cgtagcgtga cagcaaactg tttaaagtcg atcccgccaa
    17221 ttaataagcc cagaggtggc atgatgatat cggcaaccag tgaagataca atcttcccga
    17281 atgccgctcc gataatgaca cccactgcca aatccaccac gttcccgcgc atcgcaaatt
    17341 cgcgaaattc tttaataatg ctcatgttat tctccctatg aagccgacaa ccataagtct
    17401 aacaaatgtt aagccatttt cctgccagga atggatttaa ttaatcctta ataatcaaag
    17461 ggaaataata aggcgtcatt agacgcctta ttaattacaa gaagaaagga cttggctgga
    17521 agagtctttc gacgtcggta ataaactttt tatctgtgag gaacataatt acgtgatcgc
    17581 cttgctcaat gcgcagattg tcattggcaa tcatcacatc gtttccacgt accaccgctc
    17641 caataatcgt tcctggcggt agcttgattt cgtcaatgac tctgccgaca acgcgtgagg
    17701 tgctttcatc accgtgagca acggcttcaa tagcttctgc tacgccgcgg cgtaatgagg
    17761 aaacaccaac aatatctgct tttcgcacat ggctaagcaa cgcagaaata gttgcttgtt
    17821 gtggtgaaat cgcaatatcg atcacgctcc cctgaaccag atccacataa gcgcgacgtt
    17881 ggatcaatac catcaccttt ttcgcaccca tacgtttggc aagcatggcg gacatgatat
    17941 tggcctcgtc atcgttggtg acggcaataa acagatcaac ttgatcgata tgttcttcgg
    18001 ccagcagttc ttgatccgac gcatcaccaa aaaagacgat cgtattctgc aacttttccg
    18061 ccagttcggc agcgcgctgc tgattacgtt cgatgagttt aacgctgtaa tctttttcca
    18121 gacgacgcgc cagccctgca ccgatattac cgccaccaac cagcatgatc cgcttatacg
    18181 gtttttccag tcgctgtaat tcactcatca ccgcacggat atgctgtgaa gcggcaataa
    18241 agaacacttc atcaccagct tcaacaatgg tcgaaccttg cggacgaatg gggcgatcgt
    18301 ggcggaaaat tgctgccaca cgagtatcga tatgtggcat atgttcgcgc atggtcgaaa
    18361 gtgcattacc aatcagcggg ccgccataat aggctttaac cacagccagg ctgactttac
    18421 cctcagcgaa gttcaccacc tgcaatgcgc cgggatactc aatcagtcgg taaatattat
    18481 cgataaccaa ctgctctggt gcgatcagat gatcaatcgg tacagcatct gaatgaaata
    18541 gtttatcggc atcgcgcacg tagtctggtg agcggatacg agcgatgcga ttaggggtgt
    18601 tgaaaagtga gtaggctacc tggcaggcaa ccatattggt ttcatctgaa ctggttacag
    18661 caaccagcat atcggcgtcg tcggcacctg cctcccgcaa tacgcgtgga tgagagccat
    18721 gcccctgcac gacccgcagg tcaaatttat cctgtaaggt ccgcagacgc tcaccgttgg
    18781 tatcgacaac agtaatatcg ttgttctcgc caaccaggtt ttccgccagt gtgccgccaa
    18841 cctggccggc acccagaatg ataattttca tcagtcgcga cccgttatct catcactttt
    18901 tgattagctt agcgtaaaag aagccgtcgc cctcttcggc accgggcaaa ttttgtttac
    18961 ccggttgctc tggtgttcct gtttcacaaa gttcggcatc agcggtacgt tgcaaaaagg
    19021 ctttaatctg caggctattt tcttccggta acaccgaaca ggtggcatag accagggttc
    19081 caccggattt taaatgtggc caaatggcat cgagtatttc agactgcaat tgcgcgagtt
    19141 ccgggatatc gcgatcacga cgtaaccatt taatatccgg atggcgacga atcacccccg
    19201 ttgctgaaca aggcgcatct aataaaatgc gatcaaactg ttgctcgcca caccattggg
    19261 aagggttgcg gccatcacct tgtttcacgg tcgccttcat accaaggcgt tttaaattgt
    19321 cgtaaacgcg agagaggcgc tgctcatcaa tatcgaccgc cagaacttgc gcctctggag
    19381 caacctcaag aatatgcgtt gttttaccgc ctggggcggc gcaaagatcc agaatgtgtt
    19441 caccattttg tggagcaagc caggtcatgc aaccttgtgc tgaagcatcc tgaacggtaa
    19501 cccatccttc ttcaaaaccg ggtagcgcat gaacaggtgc aggtgtttcc agctgtacag
    19561 catcagggta atccgcatgc gggaaacctt tcattcctgc ttcatccagc aatgcaagcc
    19621 agctgtcgcg ggaatgatgc gtacgattaa cgcgcagcca cattggcgga cgctggttat
    19681 tggcttcgac gatggattgc cactgctcgg gatacgcctt ttgcagacgc ttcagcaacc
    19741 aggaaggatg cagataacgt gcatcactgg cattaaactc ggctaataac tcatcttgct
    19801 gacgctggaa ctgacgtaat acaccgttaa tcaatccctt aagttgcggg cgcttaattg
    19861 caactgcgcc ttcaaccgtt tcagccagcg cggcatgagg tggaatgcgg gtataaagca
    19921 gttgatacaa accaaccata atcaggtaat gaacagtacg ctgtttgcct gtcatcggac
    19981 gggccattag cttattaatc agccagtcta actgtgagag cgtacgcagt acgccaaagc
    20041 ataactcctg gagaagagct ttatctttat cggaaacttt ttgctggagc ggtggcagaa
    20101 tgttgctcag tgattgccct tgctcaacga cttgttcaat ggcctgggcc gccatgctac
    20161 gtaaattacg ttgttttttc ataagtataa atataaagat gcccggtaag accgggctta
    20221 gaagagtgga ctatcaggcc agacggttgc ccggtacaaa ccattcccga cgagaattca
    20281 ggaggtcttg cacgctcatc gctttcttac ctgcaggttg cattgagagc agattcagga
    20341 tgccatcacc tgtcgctacc tgaatgcctt gtttattggc ttcaaggatt gttcctggcg
    20401 cagcctttgt tgtcgtatcg atgacagatg ctttccagac tttaaccggc tgcccttcaa
    20461 tttccagcca gctcattggc catggattga aagcgcgaat gcaacgttca agctgtgctg
    20521 ccgaaagtga ccagtcaata cgcgcttctt ctttactcaa cttttcggcg taagtgacaa
    20581 gagtttcgtc ctgaacttct ggtttcgccg tgccgtctgc cagttgtttc aacgtggtga
    20641 taagcccttg tgggccaagc tctgccagct tgtcgtacag cgtaccactg gtatcttctg
    20701 cagtaatcgg gcaggagagc ttatagagca tgtcaccggt gtctaaacca acatccattt
    20761 gcataatggt cacaccagtt tctgcatcac ccgcccatag tgagcgttgg attggtgctg
    20821 caccgcgcca gcgtggcagc agtgaaccat gaacgttgat acagccaaga cgcggcatct
    20881 ccagcacagc tttaggcaga attaaaccat aggcgacgac gaccataaca tcagcctgca
    20941 gatcggcgac cagttgctgg ttttcttgtg gacgcaggga aacaggttga aaaacgggca
    21001 gacctttgtc ctcagccaga actttaaccg ggctgggcat cagtttttta ccgcgtcctg
    21061 ccggtcggtc tggctgggtg aacacgccaa cgatgttatg accagaagac aacagcgcgt
    21121 cgagatgacg cgctgcaaag tcaggtgtac ccgcaaaaat aatacgtagt gattctgaca
    21181 cgttaattct tatccttaag cccgggcttt cagacgatcc agtttttcaa ctttctgacg
    21241 aatacgttgt tgtttcagcg gtgacagata atccataaac agtttgccga ccaggtgatc
    21301 catctcatgc tgaatacaga tggctaacag accgtctgct tccagttcaa atggtttacc
    21361 gtcgcggtcc agggcgcgaa ttttaacttt ctctgcgcgt ggcactaaag cacgttgttc
    21421 agggatcgac aggcaacctt cttcaatgcc tgtttcgccg cttttttcta aaagctccgg
    21481 attgattaac accagccgtt cgtcacggtt ttccgaaaca tcaattacaa tgatacgttg
    21541 atggatatca acctgggttg ccgccaggcc aataccttct tctgcgtaca tcgtctcgaa
    21601 catatcatcg acgatacgct gaatttctgc attcacttct tctaccggtt tagcaacttt
    21661 gcgaagccgc tcgtccggaa tatgtaacac ttgcaaaact gacataaatc tccagagatg
    21721 tgttcaggag ttagaaagat tatttcttct attctagaca aatccccctc tgattgacag
    21781 catcactgac caatcgcaaa gattgctaag gctgcttatg gcagggagat aaggatggtc
    21841 gatacagaaa tttggctgcg tttaatgagc atcagcagct tgtatggcga tgatatggtc
    21901 cgtatagctc actggctggc aaaacagtcg catattgatg cggttgtatt gcagcaaaca
    21961 gggcttacat tgcggcaggc acaacgcttt ctttcatttc cgcgaaagag tatcgaaagc
    22021 tcactttgtt ggttggagca acccaaccat catttaatcc ctgcggacag cgaattttat
    22081 cctcctcaac ttctggcgac gacagattac cccggcgcac tgtttgttga aggagaactg
    22141 cacgcgttgc attcatttca gcttgccgta gtggggagtc gggcgcattc atggtatggc
    22201 gagcgatggg gacgattatt ttgcgaaact ctggcgacgc gtggagtgac aattacgagt
    22261 ggactggcgc gtggaatcga tggtgtggcg cataaagcgg ccttacaggt aaatggcgtt
    22321 agcattgctg tattggggaa tggacttaat accattcatc cccgccgcca tgctccactg
    22381 gctgccagtc tacttgagca aggtggtgct ctcgtctcgg aatttcccct cgatgttcca
    22441 ccccttgctt acaatttccc acgaagaaat cgcattatca gtggtctaag taagggtgta
    22501 ctggtggtgg aagcggcttt gcgcagtggt tcgctggtga cagcacgttg tgcgcttgag
    22561 caggggcgtg aagtttttgc tttgccaggt ccaataggga atccgggaag cgaagggcct
    22621 cactggttaa taaaacaagg tgcgattctt gtgacggaac cggaagaaat tctggaaaac
    22681 ttgcaatttg gattgcactg gttgccagac gcccctgaaa attcatttta ttcaccagat
    22741 caggaagacg tggcattgcc atttcctgag ctcctggcta acgtaggaga tgaggtaaca
    22801 cctgttgacg tcgtcgctga acgtgccggc caacctgtgc cagaggtagt tactcaacta
    22861 ctcgaactgg agttagcagg atggatcgca gctgtacccg gcggctatgt ccgattgagg
    22921 agggcatgcc atgttcgacg tactaatgta tttgtttgaa acctatattc acacagaagc
    22981 tgagttgcgt gtggatcaag acaaacttga acaggatctt accgacgcag gatttgatcg
    23041 agaagatatc tacaatgccc tgctttggct ggaaaagctt gcggattatc aggaaggact
    23101 ggcagaaccg atgcaactgg cctctgatcc actctccatg cgtatttata cccctgaaga
    23161 gtgtgaaaga ctggatgcca gctgtcgtgg ttttttgctt ttccttgagc aaattcaggt
    23221 gcttagcctt gaaacccgtg aaatggtgat agagcgagtg ctcgcgctgg ataccgcaga
    23281 gttcgacctg gaagacctga aatgggtaat cctgatggtg ttgttcaata ttccgggttg
    23341 cgaaaatgcg taccagcaaa tggaagaatt actctttgaa gtgaatgaag gtatgctgca
    23401 ttaatcattt ctttaattca gcataagttg ttatggcgaa atcagcactg ttcacggtgc
    23461 gtaataatga gtcctgccca aagtgcgggg ctgaactagt tattcgatcc gggaaacacg
    23521 gtccgtttct tggatgctca cagtatccgg catgtggcta cgtccgtcct ctgaaatctt
    23581 cagcggatgg acatattgtc aaagttctgg aggggcaggt ttgccctgca tgtggcgcaa
    23641 atctggtatt acgccaggga cgctttggta tgtttattgg ttgcagtaac taccctgaat
    23701 gcgaacatac cgaacttatc gataaaccgg acgaaacagc aattacatgc ccccaatgcc
    23761 ggacgggcca tctggttcag cgccgctccc gttatggcaa aacatttcac tcctgtgatc
    23821 gctacccgga gtgtcaattt gccattaact tcaaacccat agctggagaa tgccctgagt
    23881 gccattatcc gctactcatc gaaaagaaaa ccgcgcaggg tgtaaaacac ttttgtgcca
    23941 gtaaacaatg tggaaagccg atttcggcgg aataataacg tgaataataa cctgcaagga
    24001 gacgctatcg cagctgcgat agatgtcctc aatgaagaac gtgtcatcgc ctatccaacg
    24061 gaagccgttt tcggtgtcgg gtgcgatcct gatagcgaaa cagcagtgat gagactgttg
    24121 gagttaaaac ggcgtccggt tgataagggg ctgattttaa tcgcggcaaa ttacgagcag
    24181 cttaaaccct atattgatga caccatgctg actgacgcgc agcgtgaaac cattttttcc
    24241 cgctggccag gtcctgtaac ctttgtcttt cccgcgcctg cgacaacacc gcgctggttg
    24301 acgggccgct ttgattcgct tgctgtacga gtcaccgacc atccgttggt ggttgctttg
    24361 tgccaggctt atggtaaacc gctggtttct accagtgcca acttgagtgg attgccacct
    24421 tgtcgaacag tagacgaagt tcgcgcacaa tttggcgctg cgttcccggt tgtgcctggt
    24481 gaaacggggg ggcgtttaaa tccttcagaa atccgcgatg ccctgacggg tgaactgttt
    24541 cgacaggggt aacataatgg aaacctatgc tgtttttggt aatccgatag cccacagcaa
    24601 atcgccattc attcatcagc aatttgctca gcaactgaat attgaacatc cctatgggcg
    24661 tgtgttgaca cccatcaatg atttcatcaa cacactgaac actttcttta gtgctggtgg
    24721 taaaggtgcg aatgtgacgg tgccttttaa agaagaggct tttgccagag cggatgagct
    24781 tactgaacgg gcagcgttgg ctggtgctgt taataccctc aagcggttag aagatggacg
    24841 cttgctgggt gacaataccg atggtatagg tttgttaagc gatctggaac gtctgtcttt
    24901 tatccgtcct ggtttacgta ttttgctcat cggcgctggt ggagcatctc gcggcgtact
    24961 actgccactc ctttccctgg actgtgcggt gacaatcact aatcggacgg tatcccgcgc
    25021 ggaagagttg gctaaattgt ttgcgcacac tggcagtatt cacgcgttgg gtatgcacga
    25081 actggaaggt catgagtttg atctcattat taatgcaaca tccagtggca tcagtggtga
    25141 tattccggcg atcccgtcat cgctcattca tccaggcatt tattgctatg acatgttcta
    25201 tcagaaagga aaaactcctt ttctggcatg gtgtgagcag cgaggctcaa agcgtactgc
    25261 tgatggttta ggaatgctgg tggcacaagc ggctcatgcc ttccttcttt ggcatggcgt
    25321 tctgcctgat gtagaaccgg tcatcaaact attgcaacag gagttgtcag cgtgaatcag
    25381 gccatccagt ttccggatag ggaagagtgg gacgagaata aaaaatgtgt atgttttccc
    25441 gctctcgtga atggtatgca actgacatgt gcgatctctg gcgagagtct ggcgtatcgc
    25501 tttactggag atacaccaga acagtggtta gagagttttc gtcagcatcg ctgggacctg
    25561 gaagaagaag cggaaaactt aattcaggaa caaagtgaag atgatcaagg ttgggtgtgg
    25621 ttaccctgat ccatatattc gtccttccat ttcacgtagt tatttgcgga atagcgtaac
    25681 ccagccttct cttcatcact taacgggcgg atctgtttga cggggctacc gagatacaga
    25741 tatccgctct ccagccgttt attttgtggg accaggctac ctgcaccaat catcacatca
    25801 tcttctacta ttgcgccatc aagtaaaatt gagcccatcc caaccaaaac tcgattgcca
    25861 atggtgcagc cgtggagcat caccttgtga ccaatagtga catcttcgcc aatggttaat
    25921 gggttgccag ctgggttgta cgaggattta tgagtgacgt gcaacatact gccatcctgg
    25981 atattggtgc gtgctccgat ctgtacataa tgtacatctc cacgaatcac aacgagcggc
    26041 cagatcccca catcatcagc cagacgaacg tcaccaatca cgacgctgct atcgtcgatc
    26101 attacgcgct gaccgatttg tggaaaaaga tcgcggtatg ggcgtaaaac ctcagacata
    26161 ctcacctcaa caataaatga tttactaatg actttgggta cattattggc cttgtgcaag
    26221 tcttttagta tgcaaaaaag caccgttttg tgtgcgaatg cagcaaaaag ggtgaaaaaa
    26281 caacaaacag aaaaaaagat caaaaaagta cttgtgcaaa aaattgggat ccctataatg
    26341 cgcctccgtt gagacgacaa cgtgaaacac ttcacaagat agtcgtaaca acaaagagaa
    26401 aaatcctgaa attcagggtt gactctgaaa gaggaaagcg taatatacgc cacctcgcga
    26461 cagtgagctg aaa
//
LOCUS       DACADF010000063        26088 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.063, whole genome shotgun sequence.
ACCESSION   DACADF010000063 DACADF010000000
VERSION     DACADF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.063"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            47..388
                     /locus_tag="ISB30_002431"
     CDS             47..388
                     /locus_tag="ISB30_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990058.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            434..871
                     /locus_tag="ISB30_002432"
     CDS             434..871
                     /locus_tag="ISB30_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAP1990059.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(913..1908)
                     /locus_tag="ISB30_002433"
     CDS             complement(913..1908)
                     /locus_tag="ISB30_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP1990060.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVINAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAVSLLLSMLVQRIDRNRLVS"
     gene            2083..2382
                     /locus_tag="ISB30_002434"
     CDS             2083..2382
                     /locus_tag="ISB30_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990061.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2477..3388
                     /gene="glyQ"
                     /locus_tag="ISB30_002435"
     CDS             2477..3388
                     /gene="glyQ"
                     /locus_tag="ISB30_002435"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP1990062.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3398..5467
                     /gene="glyS"
                     /locus_tag="ISB30_002436"
     CDS             3398..5467
                     /gene="glyS"
                     /locus_tag="ISB30_002436"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP1990063.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            5789..5941
                     /gene="hokA"
                     /locus_tag="ISB30_002437"
     CDS             5789..5941
                     /gene="hokA"
                     /locus_tag="ISB30_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAP1990064.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQENYELAAFL
                     ACKLKE"
     gene            complement(6129..6341)
                     /gene="cspA"
                     /locus_tag="ISB30_002438"
     CDS             complement(6129..6341)
                     /gene="cspA"
                     /locus_tag="ISB30_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAP1990065.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(6622..6912)
                     /locus_tag="ISB30_002439"
     CDS             complement(6622..6912)
                     /locus_tag="ISB30_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAP1990066.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            7346..8056
                     /locus_tag="ISB30_002440"
     CDS             7346..8056
                     /locus_tag="ISB30_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAP1990067.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(8106..9080)
                     /gene="ghrB"
                     /locus_tag="ISB30_002441"
     CDS             complement(8106..9080)
                     /gene="ghrB"
                     /locus_tag="ISB30_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAP1990068.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVALPHIGSATHETRYGMAACAVDNLINALQGKVEKNCVNPHVVD"
     gene            complement(9184..9843)
                     /locus_tag="ISB30_002442"
     CDS             complement(9184..9843)
                     /locus_tag="ISB30_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAP1990069.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGSHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            9996..12329
                     /locus_tag="ISB30_002443"
     CDS             9996..12329
                     /locus_tag="ISB30_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAP1990070.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLAP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTVTAFEPPASS"
     gene            complement(12298..12738)
                     /locus_tag="ISB30_002444"
     CDS             complement(12298..12738)
                     /locus_tag="ISB30_002444"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1990071.1"
                     /translation="MIREAQRSELPALLELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(12735..13298)
                     /gene="tag"
                     /locus_tag="ISB30_002445"
     CDS             complement(12735..13298)
                     /gene="tag"
                     /locus_tag="ISB30_002445"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP1990072.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            13456..14154
                     /locus_tag="ISB30_002446"
     CDS             13456..14154
                     /locus_tag="ISB30_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1990073.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            14383..15591
                     /locus_tag="ISB30_002447"
     CDS             14383..15591
                     /locus_tag="ISB30_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1990074.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            15915..17606
                     /gene="eptB"
                     /locus_tag="ISB30_002448"
     CDS             15915..17606
                     /gene="eptB"
                     /locus_tag="ISB30_002448"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAP1990075.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            17698..17774
                     /locus_tag="ISB30_002449"
     tRNA            17698..17774
                     /locus_tag="ISB30_002449"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17732..17734,aa:Pro,seq:cgg)
     gene            18518..20125
                     /gene="dppA"
                     /locus_tag="ISB30_002450"
     CDS             18518..20125
                     /gene="dppA"
                     /locus_tag="ISB30_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAP1990076.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            20432..21451
                     /gene="dppB"
                     /locus_tag="ISB30_002451"
     CDS             20432..21451
                     /gene="dppB"
                     /locus_tag="ISB30_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAP1990077.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            21461..22363
                     /gene="dppC"
                     /locus_tag="ISB30_002452"
     CDS             21461..22363
                     /gene="dppC"
                     /locus_tag="ISB30_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAP1990078.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            22374..23357
                     /gene="dppD"
                     /locus_tag="ISB30_002453"
     CDS             22374..23357
                     /gene="dppD"
                     /locus_tag="ISB30_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAP1990079.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            23354..24358
                     /gene="dppF"
                     /locus_tag="ISB30_002454"
     CDS             23354..24358
                     /gene="dppF"
                     /locus_tag="ISB30_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAP1990080.1"
                     /translation="MSTHEATPQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCIQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(24388..25659)
                     /locus_tag="ISB30_002455"
     CDS             complement(24388..25659)
                     /locus_tag="ISB30_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAP1990081.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSRVLTLAICTFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SQLEKLRGFKAWMILLYGILLCLSPLLKLIE"
BASE COUNT         6349 a         6388 c         6810 g         6541 t
ORIGIN      
        1 ttcgttggtt actcgtaatg tatcgctggt agatatggag atcgttatga aaacctcaaa
       61 gactgtggca aaactattat ttgttgtcgg ggcgctggtt tatctggttg ggctatggat
      121 ctcatgccca ttgttaagtg gaaaaggcta ttttcttggc gtgttaatga cggcaacttt
      181 tggcaactat gcatatcttc gcgcagaaaa acttgggcaa ctggatgatt tttttatcca
      241 cgtctgtcag ttggtagcgt taatcactat cggtctcttg tttatcggtg ttttaaacgc
      301 acctatcaat gcttatgaaa tggtgatcta tcccatcgcc ttttttgtct gcttgtttgg
      361 tcaaatgcgt ttgtttcgct cggcatgagc aacataaagt tcttacacat tcaggaatga
      421 aaggaatact gtgatggaca acaaaatatc aacctattct ccggcattta gtattgtttc
      481 atgggtagcc cttattggcg gtattgttac ctatctgtta ggactatgga atgcagagat
      541 gcagttaaat gaaaaagggt attattttgc cgtactggta ttaggtttgt tttccgcggc
      601 gtcttatcaa aagaccgttc gggacaagta tgaaggcata cctaccaccc ccatttatta
      661 tatgacttgc ctgacggtct ttattatctc tgttgcgttg ttgctggtag gtctatggaa
      721 tgcaacgtta ttactcagcg aaaaaggctt ttatgggctg gctttcttct taagcttgtt
      781 tggtgcagta gcggtgcaga agaatattcg tgatgccgga ataaacccac caaaagaaac
      841 acaggttacc caggaagaat acagcgaata attcacgtaa gcccggtcag tccaatgtga
      901 ccgggcttct acttaactca ctaatctgtt tctgtcgatt cgttgtacca gcatagaaag
      961 taataaactc actgccagcg tcgcacaaaa gatccaaata atatccagta ttggccagtt
     1021 tttaagctca atcccccggg tgcgcagtgc atggataatc aatgcatgga acccgtatat
     1081 acctaacgaa tggcgggaga ttaagccaag tccgcaaatg gtacgcgtat acagcgtgtt
     1141 tttaaccaga gtcaataacg cgattgcgca gataaaaacc atcggcccac agtaaagata
     1201 ccaggtatcg gcaaaatttc cgcgccactg taattcatat aatgtcccac gagagataat
     1261 aaaaaccccc gtcgcaaaca gtgcggcgct cacccacgac agtgctttat gctgtgtgtc
     1321 catcatccct atagcgcggc ccaacatgcc ataaaggatg tagtaaaaag tatcgccatt
     1381 gatatataag ttaattggca gccattcaaa accgtcgatt ttctgcggca cggtgtttgg
     1441 gttagcgatg ataccaatca ccaccattag caccagcaac atttttccgc cgacgttctt
     1501 cacctgaatc agcggtgaaa ccagataaat cactgcaatc gcgaagaaaa accataaatg
     1561 gtaaaacact ggcttttgca gcaggttttt cagcgctaac tccacattga tggaggtaaa
     1621 cagcgcaatg tagagcagcg cgattgcgct ataaaaaaac aggcataagc cgatacgcaa
     1681 gaaatgacgc gactgggcgc tgcgttcgcc aaaaaagaga tagccggaaa tcatgaaaaa
     1741 tagcggcacg ctgacacgag aggcagaatt cagaacattg gcgatatccc aggtgatggg
     1801 gctaacacta tgagcattga tcacatacca ggtagtggtg tgaatcatca ccaccattaa
     1861 acacgctatc cctcgcaggt tatcaatcca gtaaattttg ggctgcatct gtgtctctgt
     1921 atctggttaa aaaaagtctg accgataaat cattcagttg gcgcactgga ataatctgag
     1981 ttttatcact acagcttata gaggcttaag gaaattcgta agatatcagc cactataccg
     2041 atataaataa taagactcac ctgcaaacca gacggtaatt taatgatgat gaacgctttc
     2101 tttccggcaa tggcgcttat tgtgctagtg ggttgttcta caccgccacc cgtgcagaaa
     2161 gcacaacggg taaaggttga tcctctgcgt tcgttgaata tggaagcgtt atgcaaggat
     2221 caggcggcaa aacgttataa caccggcgag caaaaaatcg acgtcaccgc cttcgaacag
     2281 ttccagggaa gctatgaaat gcgcggttat accttccgga aagagcagtt tgtctgttct
     2341 tttgacgcgg atggccattt tttgcatctt tccatgcgtt aagccctgct ttttcccgtt
     2401 tcgtactgta tatcttccat ccagcgggta tactgatccc ttcctttaaa tccacacgta
     2461 tccagcacga aataatatgc aaaagtttga taccaggacc ttccagggct tgatcctgac
     2521 cttacaggat tactgggctc gccagggctg caccattgtt caaccattgg acatggaagt
     2581 cggcgcggga acctctcacc caatgacctg tctgcgcgcg ctggggccag aaccgatggc
     2641 ggctgcttat gttcagcctt ctcgtcgccc gaccgatggt cgctacggcg aaaaccccaa
     2701 ccgtttacag cactactatc agttccaggt ggtcattaag ccatcgccgg acaatattca
     2761 ggagctgtac ctcggttctc tgaaagagct gggcatggac ccgaccatcc acgacatccg
     2821 tttcgtggaa gataactggg aaaacccgac gctgggtgcc tgggggctgg gctgggaagt
     2881 gtggctaaac ggcatggaag tgacgcagtt cacttacttc cagcaggttg gtggtctgga
     2941 atgtaaaccg gttaccggtg agatcaccta cggtctggaa cgtctggcca tgtacattca
     3001 gggcgtagac agcgtttacg acctggtctg gagcgacggc ccgctgggta aaaccaccta
     3061 cggcgacgtg ttccatcaga acgaagtgga gcagtccact tacaacttcg aatacgcgga
     3121 tgtggacttc ctgttcacct gcttcgagca gtacgagaaa gaagcgcagc aactgctggc
     3181 gctggaaaat ccgctgccgc tgccagccta cgagcgtatt ctgaaagccg cccacagctt
     3241 caacctgctg gatgcgcgta aagccatctc cgtcaccgag cgtcagcgct atattctgcg
     3301 cattcgcacc ctgaccaaag cagtggcaga agcatactac gcttcccgtg aagccctcgg
     3361 cttcccgatg tgcaacaaag ataagtaaga ggcggctatg tctgagaaaa cttttctggt
     3421 ggaaatcggc actgaagagc tgccaccaaa agcactacgc agcctggctg agtcctttgc
     3481 tgcgaacttt actgcggagc tggataacgc tggcctcgca cacggcaccg ttcaatggtt
     3541 tgctgctccg cgtcgtctgg cgctgaaagt cgctaacctg gcggaggcgc aaccggatcg
     3601 tgaaatcgaa aaacgtggtc cggcaattgc tcaggcgttc gacgctgaag gcaaaccgag
     3661 taaagcggca gaaggttggg cgcgtggttg cggtattacc gttgaccagg ctgagcgtct
     3721 gactaccgat aaaggcgaat ggctgctgta tcgcgcccat gtgaagggcg aaagcaccga
     3781 agcactgctg ccgaatatgg ttgcgacttc gctggcgaaa ctgccgatcc cgaaactgat
     3841 gcgttggggc gcaagcgacg tgcactttgt gcgtccggtt cacaccgtga ccctgctgct
     3901 gggcgacaaa gttattccgg ctaccattct gggtattcag tccgatcgcg tgattcgcgg
     3961 ccaccgcttt atgggcgagc cggaattcac tatcgacaat gccgatcagt atccagaaat
     4021 tctgcgtgag cgcgggaaag tcatcgccga ttacgaagaa cgtaaggcga agattaaagc
     4081 cgatgctgaa gaagcagcac gtaagattgg cggtaacgct gatttaagcg aaagcctgct
     4141 ggaagaagtg gcttcgctgg tggagtggcc ggtcgttctg accgcgaaat tcgaagagaa
     4201 attcctcgcg gtgccgtctg aagcactggt ttacaccatg aaaggtgacc agaagtactt
     4261 cccggtgtat gcgaacgacg gcaaactgct gccgaacttt atcttcgttg ccaacatcga
     4321 atcgaaagat ccgcagcaga ttatctccgg taacgagaaa gtcgttcgtc cacgtttggc
     4381 ggatgccgag ttcttcttca ataccgaccg taaaaaacgt cttgaagata acctgccgcg
     4441 cctgcaaacc gtgttgttcc agcaacagtt ggggacgctg cgcgacaaaa ctgaccgcat
     4501 tcaggcgctg gccggctgga ttgctgaaca gattggcgct gacgttaacc acgctacccg
     4561 tgcgggcctg ctgtctaagt gtgacctgat gaccaacatg gtcttcgagt tcaccgacac
     4621 ccagggcgtt atggggatgc actacgcgcg tcacgatggc gaagcggaag acgtagctgt
     4681 cgcgctgaat gagcagtatc agccgcgctt tgccggtgat gacctgccgt ctaacccggt
     4741 agcctgtgcg ctggcgattg ctgacaagat ggataccctg gcgggtatct tcggtatcgg
     4801 ccagcatccg aaaggcgaca aagacccgtt tgcgctgcgt cgtgccgcac ttggcgtgct
     4861 gcgtattatc gttgagaaga acctcaacct tgatctgcaa acgctgaccg aagaagcagt
     4921 gcgtctgtat ggcgataagc tgactaatgc caacgtggtt gatgatgtta tcgactttat
     4981 gctcggtcgc ttccgcgcct ggtatcagga cgaaggttac accgttgaca ccatccaggc
     5041 ggtactggcg cgtcgtccga ctcgtccggc tgatttcgat gcccgtatga aagcggtatc
     5101 gcacttccgt accctggaag cagctgcagc actggcggcg gcgaacaagc gtgtatctaa
     5161 catcctggcg aaatctgatg aagtgctgag cgatcgcgtg aatgcctcta ctctgaaaga
     5221 gccggaagaa attaaactgg cgatgcaggt tgtggtgcta cgtgacaagc tggagccgta
     5281 ctttgcggaa ggtcgttacc aggatgcgct ggtcgaactg gctgagctgc gtgaaccggt
     5341 cgatgctttc ttcgataaag tgatggtcat ggttgatgat aaagaattgc gtatcaaccg
     5401 tctgaccatg ctggaaaaac tgcgcgaact gttcctgcgc gttgcggata tttcgctgtt
     5461 gcagtaataa cgccgttatt aaaaagcctg ccatctggca ggcttttttt atcgctaaat
     5521 aatacagcaa cctttaataa tcttctgctg aataaagatt atctcatata ttaattttat
     5581 gagatttttt taggattata tcaaggagaa gaaacgaact tattaagctg gaatagccac
     5641 gggtgcttga ggctgtttgt ctcaggtatt caccgaaagg cagacagaga aaagccccac
     5701 ctgactataa atcaaagtga ggctacccta tgcctgaaca ccataaggtt agcctcttac
     5761 tcgttggaaa tcaacacagg aggctgggat gccgcagaaa tatagattac tttctttaat
     5821 agtgatttgt ttcacgcttt tatttttcac ctggatgata agagattcac tgtgtgaatt
     5881 gcatattaaa caggagaatt atgagctggc cgcgttttta gcctgcaaat tgaaagagta
     5941 agaatcttcg gcgggaagtt attcccgcct ttcttacggc gttacgcatt ctcattacac
     6001 ccaaatttat tcttcacaaa aataataata gattttatta cgtgaccgat tatttatttc
     6061 ctgaaaaaca aataaaaaaa tccccgccaa atggcaggga tcttagattc tgtgctttta
     6121 agcagagatt acaggctggt tacgttacca gctgccgggc ctttagcgcc gctttcgatg
     6181 gtgaaggaca ctttctgacc ttcgtccaga gatttgtaac catcgttctg gatagcagag
     6241 aagtgtacga acacgtcttt agagccatcg tcaggagtga tgaagccgaa gcctttgtca
     6301 gcgttgaacc attttacgat accagtcatt ttaccggaca tagtgtatta cctttaataa
     6361 ttaattgtgc ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt
     6421 gcactacgag gggtatcaac gataactctt gaagggactt gccttactac actgctttaa
     6481 tggtctgtac gtcaaaccgt tgatgtgcat taagccacgc attggtgggt gatgcaacaa
     6541 ttatttttca tatttatgat taatcggttg gaaaacggtc ctgttatcag gaccgtaaac
     6601 agaaataaag tggataaaag cctattccat caactgctta cttaatgccg ggttggcttg
     6661 aatcaaacgc atcaatttta gttcggcact tgaaggcttc acgcgtctgg attcccattc
     6721 ctttaccatg gcgactgata cgcccaaaac ccgggcgaaa tcatcgattt ttaatcctgt
     6781 cccttttcgt aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt
     6841 ctgcgtttca tctttaaaaa caatctgttc caggctgctc aacagctcat gcattggatc
     6901 tttatattcc attgaaaact cctcaaatca cactgcggga tcgtgaattg catcgaagct
     6961 cattaagaat agtcgggaaa aacagacaca gcggggtgta gtcagggcat actctgacta
     7021 tgattcgatt cagccagtca tttagcgtga cttatagcca cctaacgatc tgataaatgc
     7081 cgatatatct cttaccactc ccggtttgta aagatttcga aaatctaacg ttctgcgaag
     7141 gcgtaaatct ctggctacaa aaaaggcaac ggcaatatca ttttttaaca atgaaacaac
     7201 aaattaacct tgtttcattt tctccatgat tttaccagga agattatgta gaaagggtat
     7261 cctgcaagcc tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct
     7321 ttttttgggt gaaaggagta agaaaatggc gacaggaaag tcctgctctc gctggtttgc
     7381 gcctcttgcg gcgttattaa tggtagttag cctgagtggg tgttttgata aagaaggcga
     7441 tcagcgtaaa gcgtttatcg acttcctgca gaatacagtg atgcgtagcg gtgaacgtct
     7501 accaaccctg actgccgatc agaaaaaaca gtttggtcct tttgtctctg attacgcgat
     7561 tctgtatggt tattctcagc aggtgaatca ggcgatggat tccggtctgc gcccggttgt
     7621 agacagcgtt aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg gtccgctgcg
     7681 tgaaatgaac ggttcgctgg gtgtgctggc gcaacagctg caaaatgcga agttgcaggc
     7741 cgatgctgcg cactctgcgt taaaacagag tgatgacctg aaaccggtct ttgatcaggc
     7801 attcaccaaa gtggtaacga cgccagctga tgcattgcaa ccgttgattc cggcggcaca
     7861 aactttcacg caacaactgg tgatggttgg agactacatt gctcagcaag gtactcaggt
     7921 gagctttgtc gcaaatggca tccagttccc gacttcacag caggcaagtg aatataacaa
     7981 actgattgcg ccattaccgg cacaacatca ggcgtttaat caggcctgga ctacagcagt
     8041 tactgcaacc caataaagag taaaagcccg agcgattatc gggctttact cctcgcagtc
     8101 gcggcttagt ccacgacgtg cggattcaca cagttcttct caacctttcc ttgtaacgca
     8161 ttaatcaaat tatccacggc acaggcggcc atgccataac gcgtctcatg ggtggcagat
     8221 ccaatatgcg gtaatgcgac gacgttggcc attgagagca acggcgaatc tacggacagt
     8281 ggctcttgtt cgaagacatc cagcccggcg gcgtgaattt cccctttctg caatgcagca
     8341 atcagtgcat tttcatcaac caccgggcca cgtccggcat taatgaaaat ggcggaggat
     8401 ttcattttgg cgaattgttc tgcgccaaac agatgatgcg tctcatccgt taacggcagg
     8461 atcaggcaaa cgaaatctga ctcttgtaac agagtatcca aatcgcagta gcgggcgttg
     8521 aagcgttctt ctgcttcttt atggtggcga cgcgcgttat agaggatggg catgttgaag
     8581 ccaaagtgcg cacgttgtgc cagcgccatg ccgatccgtc ccatcccgac aatgcccagt
     8641 gttttatggt gaacgtcagt gccgtaccag tccgggccta tactcgcggt ccattcgcct
     8701 gcttttaccc gttctgctac ctccacaacc cgacgagcgg tagacaacac cagcgccatc
     8761 agcgtatcgg cgacggtttc tgttaatacg gttggtgtgt gcatcagcag aatttttcgg
     8821 gcggtaagcg catcgacatc aaaattgtca tagccgacgg agatcgttga tgtggcacgc
     8881 agtttcggca ttttttccag caatgcggca tcaacattct catttgaacc cagcaagcct
     8941 tcagcttcgg caaaaattgc tgcattttgt tcgacggttt gtgggctgag gtttgctacc
     9001 tggtgaacgg tgaaatgttc ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag
     9061 aggataacgg acggcttcat gcttctctcc attcactgat aaggcgatca ccttagcctg
     9121 aaatatgatg aaggaaaact gaagaatgcc aggtgatttc tgcatcacct gacatgaaag
     9181 ggattacagt gggcttaagg taatttctac acggcggttt tgcgccttac cttctgcggt
     9241 gctgttgctg gcgattgggt tagccgggcc aaggccctga gtacggatgc ggctggcgtc
     9301 cacgccctgg gtgatcaacg cgctggcaac ggaatccgca cgctgctggg agagacgcat
     9361 gttcaggtcg tgactccccg tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg
     9421 atactctttc agtaccattg ccacgccggt cagggtgtta gcgcccgccg gtttcagggt
     9481 tgcgctgctg ctgtcgaagg tcacattgtt cggcatattg aggataatgt tatccccgct
     9541 gcgggttacg ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc
     9601 catgtaataa ccaacgccgc cgcccagagc tgcgcctgct gctgcgccaa tgagcgcgcc
     9661 tttaccgcga tctttcttcg aagaagagag cgcaccaata cccgcgccaa cgagagagcc
     9721 cagacctgcg ccgatagcag atttacctgc ttcgcgttcg ccggtgtaag ggttagttgt
     9781 acagccagat accgccagag cgccactcac tacggcggca ataagataaa cacgtttctt
     9841 cattgttaat ccttaataac ctttttattc tttgccacgg gttccgtggc gggagattat
     9901 gccgcgtgag catgaagatt attcctggga atactcggaa atttgtaagt aatatttaac
     9961 tgctcaatac atctaacctt tcaggagtct tcggtttggc caactcatcc tcacgatatt
    10021 ccgttctgac tgccgcccac tgggggccta tgctggttga aaccgacggc gaaaccgtgt
    10081 ttagctcgcg tggcgcgtta gccacaggaa tggaaaactc cttgcagagc gcggttcgcg
    10141 accaggttca cagcaatacg cgggtgcgat ttccaatggt gcgcaaaggc tttcttgcgt
    10201 caccggaaaa tccgcaaggc attcgtgggc aggatgaatt tgttcgcgtg agttgggatg
    10261 aggcgctgga gcttattcac catcaacata aacgcattcg tgaggcttat ggtccggcat
    10321 cgatttttgc tggttcctac ggctggcgtt caaacggcgt gctgcataag gcctcgacat
    10381 tattacaacg ctatatggcg ttggcaggcg gttatactgg gcatctgggg gattattcga
    10441 ccggcgcggc acaggcgatc atgccgtatg tcgtgggtgg cagcgaagtt tatcaacagc
    10501 aaaccagttg gccgctggtg ctggaacata gcgatgtcgt ggtgctgtgg agtgctaacc
    10561 cactcaatac gctgaaaatt gcgtggaatg catccgatga gcaggggctt tcttactttt
    10621 ccgcactgcg tgacagcggg aaaaagctga tctgcattga tccaatgcga tcggaaaccg
    10681 tcgatttctt tggcgataaa atggagtggg tggcaccgca catgggcacc gatgttgcgt
    10741 tgatgctggg gatcgcccat acgctggtgg aaaatggttg gcacgacgaa gcgtttctgg
    10801 cgcgttgcac cacaggttat gctgtcttcg cctcttattt gctgggcgag agtgacggaa
    10861 tagcgaaaaa cgccgaatgg gcggcagaga tttgtggtgt tggcgcagcg aaaatccgcg
    10921 agctggcggc tattttccac caaaatacaa ccatgctgat ggcaggctgg ggaatgcagc
    10981 gccaacagtt tggtgagcaa aaacactgga tgatcgtcac gctggcggca atgttggggc
    11041 aaatcggcac acccggcggc ggttttggtc tttcttacca ttttgccaat ggtggtaacc
    11101 ccacgcgccg cgctgcggtg ctctcttcca tgcagggcag cttgccgggt ggcaccgatg
    11161 cggtagataa aatccctgtc gcccgcattg ttgaagcact ggaaaaccct ggtggcgcat
    11221 atcaacataa cggtatggac cgacatttcc cggatattcg ttttatctgg tgggcgggtg
    11281 gtgccaactt tactcatcat caggatacca atcgcctgat ccgtgcctgg caaaaaccgg
    11341 agctggtggt gatctccgaa tgcttctgga cggctgcggc gaaacacgcg gatatcgttc
    11401 tgcctgcgac cacctctttt gagcgtaatg atctcaccat gaccggtgat tacagtaatc
    11461 agcatctggt gccgatgaag caagtggtgc cgccacgcga tgaagcgcgt aatgattttg
    11521 atgtttttgc cgagttgagt gaacgttggg agaagggcgg ttatgcacgt tttacggaag
    11581 gaaaaagtga gctgcaatgg ctggaaacgt tttataacgt tgcccggcag cgcggggcaa
    11641 gccagcaggt tgaattgccg ccatttgctg agttctggca agccaaccag ttaattgaga
    11701 tgccggaaaa cccggacagt gagcggttta ttcgcttcgc cgattttcgc cgcgatccgc
    11761 aggcgcatcc attaaaaacc gccagcggta agattgaaat cttctcgcag cgtattgccg
    11821 attacgctta tccggattgc ccggggcatc caatgtggct ggcgccggac gaatggcagg
    11881 gtaatgccga accggaacag ttgcaggtac tttctgctca tccggcgcat cgtctgcaca
    11941 gccagctgaa ttacagttct ctgcgcgaat tgtacgcggt ggcaaatcgt gagcctgtca
    12001 ccattcatcc tgacgatgcc cagacgcgcg gcatacaaga tggcgatact gttcggctgt
    12061 ggaactcacg cgggcaaatt cttgccggcg cggtcattag cgagggaatt aaacctggcg
    12121 tgatttgtat tcacgaaggc gcatggccgg atttggattt aaccgctgac ggcatttgta
    12181 aaaacggcgc agtgaacgtg ctgaccaaag atctccccag ctcgcggctg gggaatggct
    12241 gtgcgggtaa tacggcgctg gcatggctgg aaaaatacaa cggtccggaa ctgacagtta
    12301 cagcgtttga accaccggcc agctcataat ccaggtgggt agttgggttt catcctgcca
    12361 tgcgcaatcg acaatgtgaa aaccctgcgc atggtagaaa tctatcgccg gttgattttt
    12421 ttgataaacc tccagcatca gatggggatg gcgctgctgc acatactgca tcagcgcctt
    12481 accaataccg cgcctgacgg ccttcggtgc gacaaacatt gctgccagaa atcggccttc
    12541 cataatgctg acaaaaccga gaagcttacc gtcttcttcc cagacccagt tttgcgcgtt
    12601 ggcaagatac gcatcccgca ccagcggaat gcagtcacgc cagtaactcg attttataaa
    12661 gggatgcccc caggttgtac tttccagcca cagttcgagg agcgcgggga gttctgaacg
    12721 ttgcgcttcc cgaatcatgg tttatttccc ggatggcaac agcagccaac cacatgatca
    12781 ttcaccagcc cacatgcctg cataaaggag taacagattg tggtaccgac aaacttaaaa
    12841 ccacgttttt ttagtgcctt agatagggcg tcggaggcgg gtgtagatgt ggggatttcg
    12901 ctcaacgttg tggcttgtgt cacctgtggt tgatgattga caaacgacca gacaaagtcg
    12961 gcaaacggtt cgccgttctg ttccatttgc aggtacgccc gcgcattacc aataattgcc
    13021 tgaattttcc cacgatggcg gataatcccg gcgtcctgta ccagtctttc gacatcctct
    13081 tcctgcattg cggcaacttt caccggatcg aactgatgaa agcatgcgcg atagttttcg
    13141 cgttttttga ggacggtgat ccacgataac ccagcctgct gcccttccag gcagatcatt
    13201 tcgaacagtt ttttactgtc agtttcaggc acgccccatt cattatcatg gtaggcaata
    13261 taaagcgggt cctgactcac ccagccgcaa cgttccatac tttccctcgc tatgtacccg
    13321 atcgtaaaaa tttcagccgc ttttcttgac gcatcggtga aaaagtcaat atttaataca
    13381 gggctactat gcccgatatc cttcatacaa tgacaataat attgccgaat tcggctcttc
    13441 cctggagtcg tgttaatgat cataaaaaaa agcggtggtc gctggcagct aagcctgctt
    13501 gcgagcgtgg taatcagtgc cttttttctc aacacagctt acgcctggca acaagaatat
    13561 atcgttgata cgcaacccgg acattccaca gagcgttaca cctgggatag cgatcatcaa
    13621 cctgattaca acgatatttt gtcgcaacgt attcaaagta gccaaagggc gctgggactg
    13681 gaagtcaatc tggcggaaga aacccctgtg gatgtgacca gcagtatgag tatgggctgg
    13741 aattttcctt tatatgagca ggttacaacc ggcccggtcg cggcattaca ttacgatggc
    13801 acaaccacct cgatgtataa cgagtttggc gacagtacta ccacgctggc cgatccgtta
    13861 tggcatgcca gcgtgagtac cttaggctgg cgtgttgact cccggcttgg cgatctccga
    13921 ccctgggcgc aaatcagcta taaccagcaa tttggcgaga atatctggaa ggcgcaatca
    13981 ggcctgagcc ggatgacggc gacaaaccag aacggcaact ggctggatgt caccgtaggc
    14041 gctgatatgt tgctcaatca aaatattgct gcctatgctg cgctatctca ggcagaaaat
    14101 accactaaca atagcgacta tctgtatacg atgggggtta gtgccagatt ttaacgttat
    14161 agatacaatt gaaacccata aataacaact aatgtggcga tagtggggac taacttacta
    14221 aataataaat ttggcgaata attgtcgagt cattcattcc tgaactaatg catttcattc
    14281 cgttctgatg gcatttcatg ccgtttttcc ccaggcataa agcgcacttc gttatggttg
    14341 tcagcagaga tttttccttt ttattactgc aggaatactg ccatgacacc ttcaaattat
    14401 cagcgtactc gctggctgac actcatcggt actatcatta cccagtttgc tctggggtcg
    14461 gtttatacct ggagtctgtt taacggtgcg ctttctgcca agctgggggc tcctgtaagc
    14521 caggttgctt tctctttcgg cttgttaagt ctggggctgg caatttcgtc ttctgttgcg
    14581 ggcaaattgc aggaacgttt tggtgttaaa cgcgtcacta tggcttccgg cattttgctg
    14641 ggattaggtt tcttcctgac agcacattcc aacaacctga tgatgctgtg gttaagcgcc
    14701 ggtgtgttgg ttggtctggc ggatggtgcg ggttacctgt tgacgctctc taactgcgtg
    14761 aagtggtttc cggagcgtaa gggacttatc tctgcttttg ctatcggttc ttatggtctg
    14821 ggcagcctgg gttttaaatt tatcgacacg cacctgctcg aaacggtggg tctggaaaaa
    14881 acctttgtta tttggggcgc gattgtactg gtgatgattg tctttggcgc aacgttaatg
    14941 aaagatgcgc cgaagcagga agtgaaaacc agcaatggtg tggtggagaa ggactacacc
    15001 ctggcagagt cgatgcgtaa accgcagtac tggatgttag cggttatgtt cctgactgcg
    15061 tgcatgagtg gtctgtatgt gattggtgta gcgaaagata tcgctcaaag tctggcgcat
    15121 cttgatgcaa tttccgcagc caatgctgtg acggttattt ccatcgccaa cctttctggt
    15181 cgtctggtgc tgggcattct gtctgacaaa atcgcccgta tccgtgttat caccattggt
    15241 caggtgattg cgctggtggg tatggcggcc ctgctgtttg caccattgaa tgcagtgacg
    15301 ttctttgcag cgattgcctg tgtggcattt aactttggtg gcacgattac ggtgttcccg
    15361 tcactggtca gtgagttctt cggcctcaat aacctggcga aaaactacgg cgtcatttac
    15421 ctcggtttcg gtatcggcag catttgtggt tcgattatcg cttcactgtt tggcggattc
    15481 tatgtgactt tctacgtgat tttcgccctg ctgattctgt cactggcgct ttctacgacc
    15541 attcgccagc cagagcagaa aatgttgcgt gaggcgcatg gctcccttta atgctcagcc
    15601 gtgagctact gggcttcagc ttcagtgtgc atcaaacgca tcaacgaaat gtataaacaa
    15661 taacgcgtaa aatttgggcc agttaactgg ccctttttac tgctttttgt aacgctaaag
    15721 tctcttttca aacttgcatt tttgtaaatt tgtgcttcgt gcacactctt tccccacact
    15781 ttttcccttt gctgtggtct acttattcgc gcgtgtagat tttacttatc tgactacctc
    15841 cgcacttttc ccctgcctgg cctgaaaagc cactaagcag ggtgttatca cctgtttgtc
    15901 cagggtttgt ttgcatgaga tacatcaaat cgattacaca gcagaagctg agctttttgc
    15961 ttgcaatcta tattggcctt tttatgaatg gcgcggtttt ttaccgccgc tttggcagct
    16021 atgcgcacga ttttaccgtc tggaaaggca tttctgctgt tgttgaactg gccgccaccg
    16081 tactggtgac cttcttttta ctgcgtctgc tttcgctgtt tggccgccgc agctggcgta
    16141 ttctggcatc gctggtggtg ctcttttctg caggtgccag ctattacatg accttcctta
    16201 atgtagtcat tggttatggc atcatcgcat ccgtcatgac caccgatatc gacctgtcaa
    16261 aagaagttgt tggtctgaac tttattctct ggttaatcgc cgtaagcgcg ttgcctctta
    16321 tccttatctg gaataaccgc tgtcgttaca ccttgctccg acaactgcga accccagggc
    16381 aacgtattcg cagcctggcg gtcgtcgtac tggcaggtat tatggtttgg gcaccgattc
    16441 gtttgctgga tatccagcag aagaaagtgg agagggcgac aggtgttgat ttgccgagtt
    16501 atggcggcgt cgtcgcgaac tcttatctgc catcaaactg gctttcggca ttaggtcttt
    16561 atgcctgggc gcgggtcgat gaatcttccg ataacaattc tctccttaac cctgcgaaga
    16621 agtttacgta ccaggctcca caaaacgttg atgatacata tgtcgttttc attattggtg
    16681 aaaccacgcg ctgggatcat atgggcattt tcggctatga acgcaatacc acaccgaaac
    16741 tggctcagga gaaaaatctg gcggcgttcc gtggttactc ctgtgatact gcaaccaaac
    16801 tctcactgcg ttgcatgttt gtacgtcagg gtggagcaga agataatccg cagcgcacat
    16861 taaaagaaca gaacattttc gcggtattga agcagttagg attcagttct gatctctacg
    16921 ctatgcaaag cgaaatgtgg ttctacagca atacgatggc ggacaacatt gcttatcgtg
    16981 agcagattgg tgcggagcca cgtaaccgtg gcaaaccggt agatgatatg ttgctggtag
    17041 acgaaatgca gcaatcgctg gggcgcaacc cggatggtaa gcatctgatc attctgcata
    17101 ccaaaggctc acactttaac tacacccagc gttacccgcg cagctttgcg cagtggaagc
    17161 cggaatgtat tggtgttgat agcggctgta ccaaagcgca gatgatcaac tcatacgaca
    17221 actcggtgac ctatgtggat cactttattt ccagcgtgat tgatcaggtt cgcgataaga
    17281 aagcgattgt gttctatgca gctgaccacg gtgagtcaat taatgaacgc gagcacctgc
    17341 acggcacgcc gcgtgaactg gcaccgccgg aacagttccg tgtgccgatg atggtctgga
    17401 tgtcggataa atatctggaa aatccggtca atgcgcaggc atttgcgcag ctgaaaaaag
    17461 aagccgacat gaaagtgcca cgccgtcatg tagagctgta cgacaccatc atgggttgtc
    17521 ttggctatac ttcaccggat ggtggaatta acgaaaacaa caactggtgc cacatcccgc
    17581 aggcaagaga ggcggcggct aactaagagg cctgctgact ttctcgccga tcaaaaggca
    17641 ttttgctatt aagggattga cgagggcgta tctgcgcagt aagatgcgcc ccgcattcgg
    17701 tgattggcgc agcctggtag cgcacttcgt tcgggacgaa ggggtcggag gttcgaatcc
    17761 tctatcaccg accaaattcg aaaagcctgc tcaacgagca ggcttttttg catctgtaac
    17821 tcatgaggaa gagaaccccc gggggcgggg aggttcgact cgacgtaggc ctgataagac
    17881 gcaccagcgt cgcatcaggc aaatgtaccg gcccattgtc ggatgcggcg cgaccgcctt
    17941 atccgatcta cacaccacac atttccctgt caaatttgcg ccccatatct cactatccat
    18001 taacggattt gtgacagaaa ccattgtctt tttttatatt tgcttaaatc tttttttgcg
    18061 ttacttatgg ctggcgctgg cattttccgc tgtgcgtttt agcgttcatg gtattaatcg
    18121 ctcagatatc cacctttacc tcagattttt ttcattaaat gcataaaact taatcaccaa
    18181 atgttgctaa atattaagct gattgttctg gttgtgttgg cgtagcacaa atttctctgc
    18241 tgcaaatagc tgtttgaagt ttttttaatc tttgtttgtg gattctcacc gttggtgtaa
    18301 atcccggttg gctgtattga cgttttcaca ttctgttgac agattgtagg tcacgagggg
    18361 cattttatgg aggatccgca ctgttacact gatgttaatt agtacggcat ccccacctca
    18421 taacgttgac ccgaccgggc aaaaaacaaa aaaggtcagg cagcgacaac ccactgcaaa
    18481 gggttaaaac aacaaacatc acaattggag cagaataatg cgtatttcct tgaaaaagtc
    18541 agggatgctg aagcttggtc tcagcctggt ggctatgacc gtcgcagcaa gtgttcaggc
    18601 taaaactctg gtttattgct cagaaggatc tccggaaggg tttaacccgc agctgtttac
    18661 ctccggcacc acctatgacg cctcttccgt accgctttat aaccgtctgg ttgaatttaa
    18721 aatcggcacc actgaagtca tcccgggcct cgctgaaaag tgggaagtca gtgaagacgg
    18781 taaaacctat accttccatc tgcgtaaagg tgtgaagtgg cacgacaata aagaattcaa
    18841 accgacgcgc gaactgaatg ccgatgatgt agtgttctcg ttcgatcgtc agaaaaacgc
    18901 gcaaaacccg taccataaag tctctggcgg cagctacgaa tacttcgaag gcatgggctt
    18961 gccagagctg atcagcgaag tgaaaaaggt ggacgataac accgttcagt ttgtgctgac
    19021 tcgcccggaa gcgccgttcc tcgctgacct ggcaatggac ttcgcctcta ttctgtcaaa
    19081 agaatatgct gacgcgatga tgaaagccgg tacaccggaa aaactggacc tcaacccaat
    19141 cggtaccggc ccgttccagt tacagcagta ccagaaagat tcccgtattc gctataaagc
    19201 gtttgatggc tactggggca ccaaaccgaa gatcgatacg ctggtcttct ctattactcc
    19261 tgacgcttcc gtgcgttacg cgaaattgca gaagaacgaa tgccaggtga tgccgtaccc
    19321 gaacccggca gatatcgccc gcatgaagca ggataaatcc atcaacctga tggaaatgcc
    19381 ggggctgaac gtcggttatc tctcgtataa cgtgcagaaa aaaccactgg atgacgtgaa
    19441 agttcgccag gctctgacct acgcggtgaa caaagacgcc atcatcaaag cggtttatca
    19501 gggcgcgggc gtatcagcga aaaacctgat cccgccaacc atgtggggct ataacgacga
    19561 cgttcaggac tacacttacg atcctgaaaa agcgaaagcc ttgctgaaag aagcgggtct
    19621 ggaaaaaggt ttctccatcg acctgtgggc aatgccggta caacgtccgt ataacccgaa
    19681 tgctcgccgc atggcggaga tgattcaggc agactgggcg aaagtgggcg tgcaggccaa
    19741 aatcgtcacc tacgaatggg gtgagtacct caagcgtgcg aaagatggcg agcatcagac
    19801 ggtaatgatg ggctggactg gcgataacgg ggatccggat aacttcttcg ccaccctgtt
    19861 cagctgcgcc gcctctgaac aaggctccaa ctactcaaaa tggtgctaca aaccgtttga
    19921 agatctgatt caaccggcgc gtgctaccga cgaccacaat aaacgtgttg aactgtacaa
    19981 acaagcacag gtcgtgatgc acgatcaggc tccggcactg atcatcgctc actccaccgt
    20041 gtttgaaccg gtacgcaaag aagttaaagg ctatgtggtt gatccattag gcaaacatca
    20101 cttcgaaaat gtctctatcg aataattaaa gccatacaag actgatggca aaggcaaaac
    20161 tgcctgatgc gctccgctta tcaggcctac gaaaactctg caatgtattg agtttgcacg
    20221 attttgtagg tcggataagg cgtttgcgcc gcatccggca taaacaaagc gctctttgtc
    20281 aacaatctgc atacccggtg gcgctgtgcc tctgcgtgca gcgccatccg gcagcaatac
    20341 ttcattccct gtcccacaag ggcgggaatt gatttgtgag caatacagac acgcagttcc
    20401 aggctgcggg tcactataga gaatccgggt tatgttgcag tttattctcc gacgtttggg
    20461 actcgtcatc cccacgttta tcggtattac ccttctcaca tttgcctttg tccacatgat
    20521 cccgggcgat ccggtgatga tcatggcggg cgaacgtggg atctccccag agcgtcacgc
    20581 gcaattgctg gctgaactcg gcttagataa accgatgtgg cagcagtatc tccattacat
    20641 ttggggcgta atgcacggtg atttaggcat ttccatgaaa agccgaattc cggtatggga
    20701 agagttcgtg ccgcgctttc aggccacgct ggaacttggc gtctgcgcga tgatttttgc
    20761 gaccgcagtc gggattccgg ttggtgtgct ggctgcggtt aaacgtggtt ccattttcga
    20821 tcacacagcg gttggcctgg cgctgaccgg ctactcgatg ccaattttct ggtggggcat
    20881 gatgttgatc atgctggttt cggtgcactg gaacctgacg cctgtctccg gtcgcgtgag
    20941 cgacatggtg ttcctcgatg attccaatcc gttaaccggt tttatgctga tcgacaccgc
    21001 catctggggt gaagacggta actttatcga tgccgtcgcc catatgatcc tgcccgccat
    21061 tgtgctgggt actattccgc tggcggtcat tgtgcgtatg acgcgctcct cgatgctgga
    21121 agtgctgggc gaagattata tccgcaccgc gcgtgccaaa ggtcttaccc ggatgcgggt
    21181 gattatcgtc catgcgctgc gtaacgcgat gctgccggtg gtgaccgtta tcggcctgca
    21241 ggtgggaaca ttgctggcgg gggcgattct gactgaaacc atcttctcgt ggccgggtct
    21301 ggggcgctgg ttgattgacg cactgcaacg ccgcgattat ccggtagtgc agggcggcgt
    21361 attgctggtg gcgacgatga ttatcctcgt caacctgctg gtcgatctgc tgtacggcgt
    21421 ggtgaacccg cgtattcgtc ataagaagta agggggcatc atgtcacagg ttactgaaaa
    21481 taaagtgatt agcgcaccgg tgccgatgac cccgttacag gagttctggc actattttaa
    21541 acgcaacaaa ggcgcggttg tcgggctggt ttacgtcgtc atcgtgctgt ttatcgcgat
    21601 ctttgccaac tggattgcac cctataaccc agcggaacag ttccgcgatg cactgcttgc
    21661 cccgcctgcc tggcaggaag gcggcagcat ggcgcacttg ctgggcactg atgacgtggg
    21721 gcgtgatgtg ctgtcgcggc tgatgtacgg cgcgcgcttg tcgctgctgg ttggctgtct
    21781 ggtagtggtg ttatcgctga ttatgggcgt tattctcggc ctgatcgctg gttactttgg
    21841 cggtctggtc gataacatca tcatgcgcgt ggtcgatatc atgctggcgc tgccaagtct
    21901 actgctggca ctggtgctgg tggcgatttt cggcccgtcg attggtaacg ccgcgctggc
    21961 gctaaccttc gttgcgctgc cgcattatgt gcgcttaacc cgcgccgccg tgctggtgga
    22021 agttaaccgc gattacgtca ccgcgtctcg cgtggcgggt gccggggcaa tgcgccagat
    22081 gtttattaac atcttcccga actgccttgc gccgctgatt gttcaggcgt cgctcggttt
    22141 ctctaacgcc attctcgata tggctgctct tggtttcctc ggcatgggcg cgcagccgcc
    22201 aacgccggag tggggcacca tgctctctga cgtgttgcag ttcgcgcaaa gcgcctggtg
    22261 ggtcgtgacc ttcccgggtc tggcgatcct gctgacggtg ctggcattta acctgatggg
    22321 tgacggtctg cgtgacgcgc tcgatcccaa actgaagcag taagaggttc gagatggcgt
    22381 tattaaatgt agataaatta tcggtgcatt tcggcgacga aagcgcaccg ttccgcgctg
    22441 tagaccgcat cagctacagc gtaaaacagg gtgaagtggt cgggattgtg ggtgagtccg
    22501 gctctggtaa atcggtcagt tcgctggcga ttatggggct gattgattat ccgggccgcg
    22561 taatggcgga aaagctggag tttaacggtc aggatttgca gcgtatctca gaaaaagagc
    22621 gccgcaacct ggtgggtgcc gaagtggcaa tgatcttcca ggacccgatg accagcctta
    22681 acccgtgcta caccgtgggt ttccagatta tggaagcgat taaggtgcat caaggcggca
    22741 acaaaagtac ccgccgtcag cgagcgattg acctgctgaa tctggtcggt attcccgatc
    22801 cggcatcgcg tctggatgtt tacccgcatc agctttccgg cggcatgagc cagcgcgtga
    22861 tgatcgccat ggcgattgcc tgtcggccaa aactgctgat tgccgatgaa ccaaccactg
    22921 cgctggacgt gaccattcag gcgcaaatta tcgaactgct gctggagcta cagcagaaag
    22981 agaacatggc gctggtgtta attacccatg acctggcgct ggtggcggaa gcggcacata
    23041 aaattatcgt gatgtatgca ggccaggtag tagaaacggg cgatgcgcac gctatcttcc
    23101 atgcgccgcg tcacccgtat actcaggcat tgctgcgtgc gctgccggaa tttgctcagg
    23161 acaaagaacg tctggcgtcg ttgccaggtg tcgttcccgg caagtacgac cgcccgaacg
    23221 gctgcctgct taacccgcgc tgcccctacg ccactgacaa atgccgtgct gaagaaccgg
    23281 cgctgaatat gctcgctgac gggcgtcagt ccaaatgcca ttacccactt gatgatgccg
    23341 ggaggccgac actatgagta cgcatgaggc caccccgcaa caaccgctgt tgcaggctat
    23401 cgacctgaaa aaacattatc cggtgaagaa aggcatgttc gcgccggaac gtctggtgaa
    23461 ggcgctggat ggcgtttcgt ttaaccttga acgtggcaaa acgctggcag tagtaggtga
    23521 atctggctgc ggtaaatcga ccctaggtcg gttgctgacg atgattgaaa cgcccaccgg
    23581 cggcgaactg tattaccagg ggcaggatct gctcaagcac gatccgcagg cgcagaagct
    23641 acgtcggcag aaaatccaga ttgtcttcca gaacccttac ggttcgctga atccgcgtaa
    23701 aaaagttggg cagattctcg aagagccgct gctgatcaac accagcttaa gcaaagaaca
    23761 gcgtcgggaa aaagccctgt cgatgatggc gaaagtcggc ctgaaaaccg agcattatga
    23821 ccgctatccg catatgttct ccggcggtca gcgtcagcgt atcgccattg cccgaggtct
    23881 gatgctcgac ccggatgtgg tgattgccga tgagccggtt tccgcgctgg acgtctcagt
    23941 gcgtgcgcag gtgctgaatc tgatgatgga tttgcagcag gaactggggc tatcttatgt
    24001 ctttatctcg cacgatctgt cggtggtgga gcacattgct gatgaagtga tggtgatgta
    24061 cctgggccgc tgcgtggaga aggggacgaa ggaccaaatc ttcaataacc cgcgccaccc
    24121 gtacactcag gcgctgctct ccgcgacacc acgcctgaac ccggacgatc gtcgcgaacg
    24181 catcaaactt accggtgaac tgccaagccc gctgaatcca ccgccaggct gcgccttcaa
    24241 cgcccgctgt cgtcggcgct tcggcccctg catccagttg cagccgcagc taaaagacta
    24301 cggcggtcaa ctggtagctt gttttgccgt tgatcaggat gaaaatccgc agcgttaact
    24361 cacaaaaccg gggccatgcg ctccggttta ctcaatcaac ttcaacagtg gcgacaagca
    24421 taacaaaatg ccgtacagca gaatcatcca ggctttaaac ccgcgaagtt tttccagttg
    24481 tgagacttta tagatcagga aaaacggaat gaggcacgac acaatgccat ataacgggct
    24541 tcccaactga aagaacacca gtaccgatac acgaaacgaa acccaaatcg tcaacgtaat
    24601 gacgataaaa gtgcagatcg ccagagtcag cacgcgtgag ttaattttcc tggtatcaat
    24661 aattcggctt aacagattga gaataatgcc tttaatggcc tcgtggaaac cgagataaat
    24721 gccaaagaat gcagtcagta cggcaaagat attcagcacc gtagaggtga tatgaatgat
    24781 atgcccaggg atcacctgcg cggccagtgc cagtgccgag atattttgtt caaaggcaga
    24841 aacggcttct tcgtggctaa ttgagaaggt aaacgaaaag gcaaaaaaca ggatcaccgc
    24901 gatgagcgtg acataactaa ttcggtgggt acgcagcgcg agccgggttg ccagtacttt
    24961 atccgcttcc cgtttacgat aggcaatatt cattgggtta agtacctgaa taaataccgc
    25021 ggaaaagaag caaaatggaa tagtaagcag gacatcgcgg aaaaagtcgg aggcttgcgg
    25081 gaaggcggtt atattggcga aattccagtg cgggatcatc gcaaaaccga acacgacaat
    25141 aatccctact ttgaccacca ccattggtcc ggaaatctta aacagtaatc gttcaccacc
    25201 agacgcaatc gccaccagta cggcgaaaat ggcgacttta taaaatagag attgagaaag
    25261 atcggcatcg gttaaaccga aggttttcag gtacgaggcg ctgtcgaaaa ccacggagag
    25321 agagtagata aaaatcccgt ggataatcat caaaaagtag ataaccccga ggaaaattcc
    25381 ccagttcttc cccaggtaat gactgataat atcggtgtag tcattacagg aatcgctttc
    25441 agaaagggtt tttaaataaa tgtcctgtac tacccaggtg gcaggataag caatgatcgc
    25501 tgcggtaata aatacccaaa ttcccttcaa gccaatttgt actggcatca gcacggttcc
    25561 ggcaccaatc gccatgccta tgcataataa aacccagccg aagtcgtagc gtgtaaacgg
    25621 caatttctga tcgtgtttcg gtagtgtgtt gtgctgcata aagtcctgtc cagaaagaag
    25681 tcggcctcgc aaaaatgaat aaacccagtt tctcttgcgc aacaggtgga gaagtcgaat
    25741 acgcagtatg taaaaaatga ggcaacagat tcactgggga ggtcgcgagg aggcgcaatt
    25801 ctacaaaatt gcgcatttca ctactagtct ggagtcagcc cggatatgag ctgcaactat
    25861 tgcaaataaa gaaattaagc tggaaggcat cacgcaatga ggaagattgc cgggtgctga
    25921 cggcaaaaag caatcgtaaa atcagcggca agccggaagg gttccggtga ggcgcaatgt
    25981 tgcgggggct ttatccctgg tggcattggt tgctggagag agaaaacccc cgcacgttgc
    26041 aggtatgcac ctgacaacat cacgggggcc attcttgcat ctagacca
//
LOCUS       DACADF010000064        25921 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.064, whole genome shotgun sequence.
ACCESSION   DACADF010000064 DACADF010000000
VERSION     DACADF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.064"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(118..258)
                     /locus_tag="ISB30_002456"
     CDS             complement(118..258)
                     /locus_tag="ISB30_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990082.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            354..1070
                     /gene="arcA"
                     /locus_tag="ISB30_002457"
     CDS             354..1070
                     /gene="arcA"
                     /locus_tag="ISB30_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAP1990083.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1130..2482)
                     /gene="creD"
                     /locus_tag="ISB30_002458"
     CDS             complement(1130..2482)
                     /gene="creD"
                     /locus_tag="ISB30_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAP1990084.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDLRKQNLKLNVALNLSGTGDLSVVPGGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLVLLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDELRIWK
                     "
     gene            complement(2540..3964)
                     /gene="creC"
                     /locus_tag="ISB30_002459"
     CDS             complement(2540..3964)
                     /gene="creC"
                     /locus_tag="ISB30_002459"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAP1990085.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEINVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3964..4653)
                     /gene="creB"
                     /locus_tag="ISB30_002460"
     CDS             complement(3964..4653)
                     /gene="creB"
                     /locus_tag="ISB30_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAP1990086.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFGVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4666..5139)
                     /gene="creA"
                     /locus_tag="ISB30_002461"
     CDS             complement(4666..5139)
                     /gene="creA"
                     /locus_tag="ISB30_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAP1990087.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5350..6219
                     /gene="robA"
                     /locus_tag="ISB30_002462"
     CDS             5350..6219
                     /gene="robA"
                     /locus_tag="ISB30_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAP1990088.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6216..6863)
                     /gene="gpmB"
                     /locus_tag="ISB30_002463"
     CDS             complement(6216..6863)
                     /gene="gpmB"
                     /locus_tag="ISB30_002463"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAP1990089.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6915..7442
                     /gene="yjjX"
                     /locus_tag="ISB30_002464"
     CDS             6915..7442
                     /gene="yjjX"
                     /locus_tag="ISB30_002464"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAP1990090.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            complement(7521..7847)
                     /gene="trpR"
                     /locus_tag="ISB30_002465"
     CDS             complement(7521..7847)
                     /gene="trpR"
                     /locus_tag="ISB30_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAP1990091.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(7937..9874)
                     /gene="sltY"
                     /locus_tag="ISB30_002466"
     CDS             complement(7937..9874)
                     /gene="sltY"
                     /locus_tag="ISB30_002466"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAP1990092.1"
                     /translation="MEKAKHVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10081..11748
                     /gene="ettA"
                     /locus_tag="ISB30_002467"
     CDS             10081..11748
                     /gene="ettA"
                     /locus_tag="ISB30_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAP1990093.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11869..13101)
                     /gene="nadR"
                     /locus_tag="ISB30_002468"
     CDS             complement(11869..13101)
                     /gene="nadR"
                     /locus_tag="ISB30_002468"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAP1990094.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13122..14504)
                     /gene="radA"
                     /locus_tag="ISB30_002469"
     CDS             complement(13122..14504)
                     /gene="radA"
                     /locus_tag="ISB30_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP1990095.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKTPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14553..15521)
                     /gene="serB"
                     /locus_tag="ISB30_002470"
     CDS             complement(14553..15521)
                     /gene="serB"
                     /locus_tag="ISB30_002470"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAP1990096.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMSGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            15627..16271
                     /locus_tag="ISB30_002471"
     CDS             15627..16271
                     /locus_tag="ISB30_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAP1990097.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16299..17315
                     /gene="lplA"
                     /locus_tag="ISB30_002472"
     CDS             16299..17315
                     /gene="lplA"
                     /locus_tag="ISB30_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HAP1990098.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFQEQEKELRELSAWMA
                     GAVR"
     gene            complement(17316..18647)
                     /gene="yjjJ"
                     /locus_tag="ISB30_002473"
     CDS             complement(17316..18647)
                     /gene="yjjJ"
                     /locus_tag="ISB30_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HAP1990099.1"
                     /translation="MSELTDLLLQGPRSAPELRQRLAISQATFSRLVARENRVIRFGK
                     ARATRYVLLRPYRGIERIPVWRVDDAGKAHKFADIRLCWPQGSCLVTGADGDEQWFDG
                     LPWYLTDLRPQGFLGRAWGRKLAAQLNLTDDIRLWQEEDVLYALTVFNGEYTGGWLVG
                     EGNYQRWITAQRPAEIPLDQKLTRYEQLASDALAGEIVGSSAGGEQPKFTCYAQTPSG
                     NKHVLVKFTVPQQTAVSQRWGDLLIAESIAAQILRDGGIHAIESTVLVTSNRQVFLEA
                     ERFDCKGNDGRLPIVSLEAVQSEFISSPGSWPEAMRHLCEQQLVTHQSVAQTEVIWAF
                     GRLIANSDMHAGNLSFYLSEPPFALTPVYDMLPMAYAPNSAGMLRDAAIEVKFDLNIS
                     KSAWLTAIPLAQQFWQTVARDPRISEAFRHIAQEMPEKIRQIEEKVTRMGG"
     gene            complement(18814..19533)
                     /gene="deoD"
                     /locus_tag="ISB30_002474"
     CDS             complement(18814..19533)
                     /gene="deoD"
                     /locus_tag="ISB30_002474"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP1990100.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(19590..20813)
                     /gene="deoB"
                     /locus_tag="ISB30_002475"
     CDS             complement(19590..20813)
                     /gene="deoB"
                     /locus_tag="ISB30_002475"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP1990101.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     HGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20865..22187)
                     /gene="deoA"
                     /locus_tag="ISB30_002476"
     CDS             complement(20865..22187)
                     /gene="deoA"
                     /locus_tag="ISB30_002476"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAP1990102.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEHRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(22265..23044)
                     /gene="deoC"
                     /locus_tag="ISB30_002477"
     CDS             complement(22265..23044)
                     /gene="deoC"
                     /locus_tag="ISB30_002477"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP1990103.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            23302..24852
                     /locus_tag="ISB30_002478"
     CDS             23302..24852
                     /locus_tag="ISB30_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAP1990104.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAHGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNVCECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLIDPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24824..25687
                     /gene="yjjW"
                     /locus_tag="ISB30_002479"
     CDS             24824..25687
                     /gene="yjjW"
                     /locus_tag="ISB30_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAP1990105.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSSGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         6087 a         7188 c         6623 g         6023 t
ORIGIN      
        1 tgacgaaagc tagcatttag atacgatcat ttcatcaaac tgttaacgtg ctacaattga
       61 acttgatata tgtcaacgaa gcgtagtttt attgggtgtc ctgcccctct tagcctgtta
      121 tattgctgtt aaaatggtta ggatgacagc cgtttttgac actgtcgggt cctgagggaa
      181 agtacccacg accaagctaa tgatgttgtt gacgttgatg gaaagtgcat caagaacgca
      241 attacgtact ttagtcatgt tacgccgatc atgttaattt gcagcatgca tcaggcaggt
      301 cagggacttt tgtacttcct gtttcgattt agttggcaat ttaggtagca aacatgcaga
      361 ccccgcacat tcttatcgtt gaagacgagt tggtaacacg caacacgttg aaaagtattt
      421 tcgaagcgga aggctatgat gttttcgaag cgacagatgg cgcggaaatg catcagatcc
      481 tctctgaata tgacatcaac ctggtgatca tggatatcaa tctgccgggt aagaacggtc
      541 ttctgttagc gcgtgaactg cgcgagcagg cgaatgttgc gttgatgttc ctgactggcc
      601 gtgacaacga agtcgataaa attctcggcc tcgaaatcgg tgcagatgac tacatcacca
      661 aaccgttcaa cccgcgtgaa ctgactattc gtgcacgcaa cctgctgtcc cgtaccatga
      721 atctgggtac tgtcagcgaa gaacgtcgta gcgttgaaag ctacaagttc aatggttggg
      781 aactggacat caacagccgt tcgttgatcg gccctgatgg cgagcagtac aagctgccgc
      841 gcagcgagtt ccgcgccatg cttcacttct gtgaaaaccc aggtaaaatt cagtcccgtg
      901 ctgaactgct gaagaaaatg accggccgtg agctgaaacc gcacgaccgt actgtagacg
      961 tgacgatccg ccgtattcgt aaacatttcg aatctacgcc ggatacgccg gaaatcatcg
     1021 ccaccattca cggtgaaggt tatcgcttct gcggtgatct ggaagattaa tcggctttac
     1081 caccgtcaaa aaaaacggcg ctttttagcg ccgtttttat ttttcaacat tatttccaga
     1141 tacgtaactc atcgtccatg gtaacttctt tactggcttt cattttcggc agtgaaaacg
     1201 cataccagtc gatattacgg gtcacaaaca tcatgccggc cagcgccacc accagcacac
     1261 tggttcccaa caacagcgcg ctatcggcag agttgagcag tccccacatc acaccatcca
     1321 gcaacaacag cacgagggta aacaacatgc tattacgcca gcctttcaat accgcctgca
     1381 aataaatacc gttcattaac gccccaatca gactggcgat tatccatgcc acagtaaaac
     1441 cggtatgttc agaaagcgcc agcaagagca aataaaacat caccaatgaa agccccacca
     1501 gcaaatattg cattgggtgt aaacgttgcg cggtgagcgt ttcaaaaaca aagaatgcca
     1561 taaaagtcag cgcaatcagc agaatggcgt acttagtcgc ccggtccgtt aattggtatt
     1621 gatcggctgg cgtcgtcact gcgacgctaa acgcggggaa gttttcccag ccggtatcat
     1681 tgcctgaagc aaaacgctca ccgagattat tagcaaacca gctactttgc cattgcgctt
     1741 gaaaacctga ctcgctaact tcccgtttgg ctggtagaaa atcacctaaa aaactgggat
     1801 gcggccagtt gctggttaag gtcatttcgc tattacgccc gccaggcacc acagaaagat
     1861 cgccggtacc gcttaaattc agcgccacat tcagcttcag gttctgcttg cgcaagtccc
     1921 cttcgggtaa agggatatgc acgccctgcc cgccttgctc taacccggtg ccgggttcaa
     1981 tggtcagcgc cgtaccgtta acttcaggcg ctttcaccac accaatacca cgcgcatccc
     2041 cgacgctaat cacaataaat ggcttgccta aggtgatatt tggcgcgtcg agttcgctaa
     2101 gacgcgaaac atcgaaatcg gcttttagcg ttaaatcact gtgccagacc tgaccggtat
     2161 agatgcctat cttgcgttct tccacattct gattgccatc aaccatcaat gactcaggta
     2221 accaaaaatg aataaaactt cgtttccgct ccacggtttt atcgtcttcc tgcaccgtat
     2281 aaagctcggt cacaggaata gcgatgagcg gcccaacgag tttttgcggc ccgctggtac
     2341 tttggcgaat cacatcttcc acatcgctac ggtaatcggc acgttcgaca atcacctgcc
     2401 gaatcagcat tatcggaatc aacaacagca atactgcacc aaacaaggtg gtcattttcc
     2461 agaacagggg ggatttcaac atagtcttct cctttgcaat gacagcagga tacgaaggct
     2521 atgtgggaag aatttgaagc tatgtgaagt gacggtgaag tcgaagcgag gccagcacgc
     2581 caccttcctg cacgttgcgc agcgtgactt cgccgttaaa caaacgggcg acctcactga
     2641 caaacgccaa ccccagaccg ctgctttttt gcccatttgc acgcggcaaa gagtaaaagc
     2701 gttcaaaaat acgtgacagc gcgtagtcag gaatcccact accggtatcc agcaccttaa
     2761 gggtgacgta ttcctgatcc acttcggcgc ttagcgttat gcgaccgctc tcgggagtaa
     2821 aatcgatggc gttatccagt aaattcccca gcgcctgctc cagtaacgcc ggttcagcag
     2881 caacgttaat ctcagtaggc ataacatgca aagtgatgtt tttttctgcc aactgcacgg
     2941 tgcgcgcttc gctgacgcgg cgaaataatg ccgccacatc aacagcagtc agaacgactt
     3001 cctgacgatt ctccagtctt gcctggcgta gtaacgtttc caccagtgcc tgcattcgcg
     3061 cattttgcgt cagaatgttg tcggtaaaac gagccaccac ttccggcggc ggaccttcgc
     3121 gtaaaatttc cgccgcgcca cgaatcgccg ccagtgggct ttttagctca tgagttaacg
     3181 cataaacata ctgctcaata tagtttttcc cttccagctt cacgcgcata ctttccagcg
     3241 cctgcgcgag tttacgcagc tcgctactac cgagatcggg gagaggaacg ggcttattgt
     3301 cagtgacgga atcagcatag cgagtgagcc gggcaataga gcggttgatc caccaaacca
     3361 tgcctgcgcc aatcaccagt gcaatcccca acaaaatggc gctggcccat aaaattcgcc
     3421 gctcgctacg cttaatgacc ggggccatcg ccgcgttcgg tttgcctacg ctcaaaacgc
     3481 caataagccg cgagccgtcc ataatcggcg cggcaacata catcacagaa ctttcgggat
     3541 cggcaggatt ttgcaacgtg ctgcgcgcac catactgacc acgcaacgtt agccagacgt
     3601 cattccagcg cgaataatcc tgtccaacgg ctttatttgc cgaatcaaac aatactttgc
     3661 cctgcgcatc ggtcatatag acatgatatt cgttgcgcac tttgttaatg ccaccgatat
     3721 tggcgcgaaa cgggcgatgt tgtagctgat taaacgcctg cgccagttgc ccatgcgttg
     3781 ggtccccaga gagcaaatcg ggacgcgcca gctccgccag caacgttgcg gtgtcgatta
     3841 acgtcccctc ggttgctctt cgcacgcccg gtttaacttc tttgacaaaa atagccagta
     3901 cgaaccaggc cgccaccgcc accagtaaaa aatagcccag caacaaccgc atgccgatac
     3961 gcattacagg cccctcaggc tatatcccat gccgcgatga gtattaatcg gtgaaagatc
     4021 ggggttgatg gcgcgcagct tggcacgcag cgttttaatg tgggtatcga cggtgcgatc
     4081 gtaggtgtcc tgcgcatctt cccacacgct atccattagt tgctggcggg accagacgcg
     4141 gcccggcgac ttgagtaacg tcttcagcaa taaaaactca taccgcgtca gcgttaatgg
     4201 cgtgtcaaac cagctgatct gcgccgcagg ttcattcaat tcaaaatgac caatacggat
     4261 aacgggagac ggcgtcgaga acttcttcac ccgacgcagt aaggtgcgca ctctggcgca
     4321 cacttcgcga ggtgaaaacg gtttagccac gtagtcgtca gcgccaattt ccagcccaag
     4381 caggcgatcg acctcttcac ttcgggccgt caggaacagt acaggtaacg ccggatggag
     4441 cgccagtaac tggcggcaca attcaaagcc gctgatatca ggcaaaccaa catcgagaat
     4501 catgacatcg ggaacctgct ggcgagcttt atccagcact ggcaagcctc gctcaaagac
     4561 ctcgacgcca aaaccttcct gttgcaacat gtagaccagc gtatcggcta tcccttgctc
     4621 gtcttctact aaccagaccg tttcccgttg cataaatcgc ctctgttatt gccgccacgg
     4681 catgacagga accgcgctaa tcgcgttttt cggcgaacct tctacaactt tgtcggagta
     4741 agccagataa gcgagcgcgt tgcgtttagc gtcataaaag cgcacgacct gtaacgactt
     4801 aaagaccagg gacgtgcgtt ttttgaatac tacctcgccc tgagctttgc cgtttttaat
     4861 acgatccgac agttcaatcg gcccgacttg ttgacaagaa atggccgcat cggaggtatc
     4921 ttctgccagg cccaatcccc ctttaatacc accggttttc gcccggctca cataacaggt
     4981 gacatttttc acatcgggat cgtcaaaggc ttcaacaaca attttgtgat ccgggccgat
     5041 cattttaaat acggtgtcga ccgaaccaat ctcttctgca tgagccaatg gccccagcat
     5101 tatcaggctt aaagaaagga ttaaatgctt gtatttcata ttgttaccat ttttatctat
     5161 tacacgagca attagttcgt cacggtaaat aacatattga ttaagaacaa aatctcaata
     5221 cttttatttc cgtcaagccc taaaacatac tcgactaaga aaaaaacact gaatgctaaa
     5281 acagcaaaaa atgctattat ccaattacct gatgtcaggt gctcgttgtt gaaaggatga
     5341 ggatatttta tggatcaggc cggcattatt cgcgaccttt taatctggct ggaaggtcat
     5401 ctggatcagc ccctgtcgct cgacaatgta gcggcgaaag caggttattc caagtggcac
     5461 ttacagagaa tgtttaaaga tgtcactggc catgctattg gcgcgtatat tcgtgctcgt
     5521 cgtttgtcga aatcggcggt cgcactacgc ctgactgcgc gtccgattct ggacatcgcg
     5581 ctgcaatacc gcttcgactc tcaacagaca tttacccgcg cattcaagaa gcagtttgcc
     5641 cagactcctg cactttaccg ccgttctcct gaatggagcg cctttggtat tcgcccgccg
     5701 ctgcgtctgg gtgaattcac tatgccagag cacaaatttg tcaccctgga agatacgccg
     5761 ctgattggtg ttacccagag ctactcctgt tcgctggagc aaatctctga tttccgccat
     5821 gaaatgcgct accagttctg gcacgatttt ctcggcaacg cgccgaccat tccgccggtg
     5881 ctctacggcc tgaatgaaac gcgtccgagt caggataaag acgatgaaca agaggtattc
     5941 tataccaccg cgttagccca ggatcaggca gatggctatg tactgacggg gcatccggtg
     6001 atgctgcagg gcggcgaata tgtgatgttt acctatgaag gtctgggaac cggcgtgcag
     6061 gagtttatcc tgacggtata tggaacatgc atgccaatgc tcaacctgac gcgccgtaaa
     6121 ggtcaggata ttgagcgata ctacccggca gaagatgcca aagcgggaga tcgcccaatt
     6181 aatctgcgct gtgaactgct gattccgatc cgtcgttaac gctgcagctc atctaatgca
     6241 ggggcgtcca gatgcgagat gtcccctgcg gtttcaacca cccagccaga cgccagccac
     6301 aggctttcct gataatccac gcgcgaaata gaacagttac gcagacgtaa gcggcgctct
     6361 gcccatgctg gtaatccgag aatcgtactc accaggcatc ccagtgcaat accatggctc
     6421 accagtagcg gtcggcttcc ctgcggtaag tcccggcaag attccagtgc cgcattaacg
     6481 cgatcgctga gctcctgcat tgactcgcct tcaggaatac gcccgtcaac ggtgccattg
     6541 accagttgcc gacgccagtt ctcttcttct tcggtgagtg aatcgatatt tcttgtttcc
     6601 agtacaccca tatttaattc acgcaggcga gaatcaaata tgatgtcaca gccgcaggcc
     6661 tgggcgatga tttccgccgt gcgccgggtg cgtcctaaat cgctactgat gatatgcgta
     6721 atgccaagct ctttggcacg ggttgccacc tgcatcgcct gttgctcacc tttggcggtc
     6781 agcgggctgt cagactggcc ctgaatacgt cgctcggcgt tccactgcgt ttcaccgtgg
     6841 cggactaggt atacctgtaa catgcttttt ttccgtatac tgctgtaacg ttaattttct
     6901 gagtaatgct gattatgcac caagttgtct gtgcgaccac caatcccgct aaaattcagg
     6961 ccattctgca ggcatttcac gagatcttcg gcgaaggatc ctgccatatt gcatccgttg
     7021 ccgtcgagag cggtgtaccg gaacagccct ttggcagtga ggaaacgcgc gctggcgcac
     7081 gaaatagggt agccaatgcc cgccgtttac ttcctgaggc tgatttttgg gtggcgattg
     7141 aagctggcat cgatggtgac agcactttca gttgggtggt gattgaaaac gccagccagc
     7201 gcggcgaggc gcgttcggct actttgccgt tgcctgcggt gattctggaa aaagtgcgtg
     7261 aaggtgaggc gcttggtccc gtgatgtcgc gttataccgg tattgatgag attggtcgta
     7321 aggaaggggc gatcggcgtg tttaccgccg ggaaactcac tcgcaccagc gtctatcacc
     7381 aggcggtgat cctggcgctg agtccgtttc ataatgccgt gtatcagcca ttgcaggcct
     7441 gattaggcgt tcacgccgca tccggcattc ggtgcacgat gcctgatgcg acgctgacgc
     7501 atcttatcag gcctacaaaa tcaatcgctt ttcagcaaca cgtcttccag ccactggcgc
     7561 agctcgacgg gcgcggcttt cagactatta gatccacgcg taatcgtcgc gatgcccgcg
     7621 ccgagttcat tttttaactc acgctggctc atttcgccgc gcaacagctc ttcgacaata
     7681 cgcacgcgag tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt taacaacggt
     7741 aaatggagat cgttttggta ggcattctta agcaggtcga caaaacgtaa ccactcctgg
     7801 tgacgctgtt ctgccatcgc tgctgaatag ggtgattgtt gggccatagt atgtcgccat
     7861 tgttagcggg ggaagcaaaa tgcctccccc ggttgtactc gctaaagagt acgatagcat
     7921 atcataaacg tgtggatcag taacgacgtc cccattccgt ggcgctcatc aacgtcggtt
     7981 tatcccccat gaaatagcgg tagtaagcgt cataagccag cacgtttttc acataaccgc
     8041 gcgtctcgga gaatggaata ctctcgacaa atgccactgc gtcgatacgc cctgcgctgt
     8101 tgccaagcca ggtccgcacc cgccctggtc cggcgttata agctgctgag gagaaaatac
     8161 ggttattgcc aaactgctga taaacatatt gcaggtaact ggtgccaatg ttgatgttcg
     8221 tttccggatc cagcaattgc ccagggctgc tataaccggg aatagagaac atcttcaccg
     8281 tatgggtcgc ggtaccaggc ataatctgca tcaggccgct ggcccctacc ggtgatttca
     8341 ctttcggatt ccaggcactc tcctgacgag caatcgccat cgcatagctt tgcgggatct
     8401 ccttaccact ggtgtagcgt ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca
     8461 gatgatccca cagcttcccg gcgatcgttg cctgaacgct aagatcccac cattggttat
     8521 tgaaagcata ccgagccagt tgagcctgct ctgtttttga cttgctcttc accagattgg
     8581 cccactcgct acgcgcggtg ttatcgagat tccagtacat caactcgcgc acgcgcgcca
     8641 tctccggccc ctgagtcagg gcgctgtcaa cattctgcgg cgctttatca atcttcagct
     8701 catactcttc gccgatgcgt tgtgcagcaa ccatcgggta gaaaccacgc tgttgcatga
     8761 gttgatgcaa aatctctttt gcttcagctt cacgtccgcg ttccagcagt aaatccgcct
     8821 gccagtaacg ccattcatct ttctctttcg cttccatagg cagacgcgcc agccaggtat
     8881 tcaggccgcg acgatcgccg gtgccaagcg ccattcgcac acggcgttca ataagcgaag
     8941 tagattgcga gcgcataatc gcgtcatcgc gccatttcgc ctgctcgtcg gtgacatcgt
     9001 tgcccatcaa acgccaggcg acaatatcgc gcagctcctg aatctgatct tcattaagct
     9061 gctgtgcctg agcaagcgac gggatcatca gccgtgcatt ttcagcatcc tgccgcgcca
     9121 cactggcaaa cgccaccgcc gccatttgac gggtaaaatc ggtcgcgccg gttgtacgcg
     9181 cgaaggtcag taccgtatta gggttgttcg ccagtgaaat gattgccgaa gcgatagtct
     9241 ggtaatcggc aggcatctgc cctgccagca ctgttaccag gccggtgtta cccgctttca
     9301 tcgccagacg gatacgctct aaatacgcca gcggatcttg tttacctgac gcacgccaga
     9361 cgctaaataa cttgtcacag gcgttaggct ggctcttgcc ggttagccac agctctttcg
     9421 ccccttgcca ggcttcttca ctctgcccgg tgttccattt cgcatagtag taattacatt
     9481 gcgcttcggt agttccgggc ttttccgggc taaaggctaa caagccacgc cagtcttcac
     9541 gccgcgccag ttcattgacg aaacgagatt gcagcgtgcg agcgggagga agcgtggggt
     9601 tagcgcgaac aaagttagtg accgtcaccg ccggttgatt catcaggtca tcagtgatct
     9661 ggcggtattc caggtaggga taaagtgggt aatctttcag tccaggcatc atttgttcga
     9721 ccacatccat ttgtcgatta tcccaggcct gcttaatttg cgcgtaacgg ctacgctgct
     9781 catccagtga gtcggctcgc gccacgctgc tgaccgtcag cagacagaca ccggcagcca
     9841 acagccgcca ggtaacgtgt ttggcttttt ccacaagcgc atcctctaag tgtaaataca
     9901 tcaatgcagc gtcatgccgc gtaatagatt tatgctaacc agtcattgcc gtttacgcca
     9961 cgttacggac acttttttac ttttacgggg tgatcgactg gctgggatta gacagggaaa
    10021 caggctacac ttcgcctttg aaaactcatc atcacgataa acatagaggc gaagtccaac
    10081 gtggctcaat tcgtttatac catgcatcgt gtcggcaaag ttgttccgcc gaaacgtcat
    10141 attttgaaaa acatctctct gagtttcttc cctggggcaa aaattggtgt cctgggtctg
    10201 aacggcgcgg gtaagtccac cctgctgcgc attatggcgg gcattgataa agacatcgaa
    10261 ggtgaagcgc gtccgcagcc agacatcaag attggttatc tgccgcagga accgcagctg
    10321 aacccggaac acaccgttcg tgagtccatt gaagaagcgg tttctgaagt ggttaacgcc
    10381 ctgaaacgcc tggatgaagt gtatgcgctg tatgccgatc cggatgccga ttttgacaag
    10441 ctggccgctg aacaaggccg tctggaagag atcattcagg ctcacgacgg tcacaacctg
    10501 aacgttcagc tggagcgtgc ggcggatgcg ctgcgtctgc cggactggga tgcgaaaatc
    10561 gctaaccttt ccggtggtga gcgtcgtcgc gtggcgttgt gccgcctgct gctggaaaaa
    10621 ccagacatgc tgctgctcga cgaaccgacc aaccacctgg atgcggaatc cgtagcgtgg
    10681 ctggagcgct tcctgcacga cttcgaaggc accgtggtgg cgattaccca cgaccgttac
    10741 ttcctcgata acgttgcggg ctggatcctc gaacttgacc gcggtgaagg tattccgtgg
    10801 gaaggtaact actcctcctg gctggagcag aaagatcagc gtctggcgca ggaagcttca
    10861 caagaagcgg cgcgtcgtaa gtcgatcgag aaagagctgg agtgggtacg tcagggaact
    10921 aaaggccgtc agtcaaaagg taaagcacgt ctggcgcgct ttgaagaact caacagcacc
    10981 gaatatcaga aacgtaacga aaccaacgaa ctgtttattc cacctggacc gcgtctgggc
    11041 gataaagtgc tggaagtcag caacctgcgt aaatcctatg gtgatcgtct gctgattgac
    11101 tccctgagct tctcgatccc gaaaggagcg atcgtcggga tcatcggtcc gaacggtgcg
    11161 ggtaaatcga cgctgttccg tatgatctct ggtcaggaac agccggacag cggcaccatc
    11221 actttgggtg aaacagtgaa actggcgtcg gttgatcagt tccgtgactc aatggataac
    11281 agcaaaaccg tttgggaaga agtttccggc gggctggata tcatgaagat cggcaacacc
    11341 gagatgccga gccgcgccta cgttggccgc tttaacttta aaggggttga tcagggtaaa
    11401 cgcgttggcg aactctccgg cggtgagcgc ggtcgtctgc atctggcgaa gctgctgcag
    11461 gttggcggca acatgctgct gctcgacgaa ccaaccaacg acctggatat cgaaaccctg
    11521 cgcgcgctgg aaaacgccct gctggagttc ccgggctgcg cgatggttat ctcgcacgac
    11581 cgttggttcc tcgaccgtat cgccacgcac attctggatt accaggatga aggtaaagtt
    11641 gagttcttcg aaggtaactt taccgagtac gaagagtaca agaaacgcac gctgggcgca
    11701 gacgcgctgg agccgaagcg tatcaagtac aagcgtattg cgaagtaatg cgtaaaatgc
    11761 cggatgcggc gtaaacgcct tatccggcct acaaaccaag caatttcaat acgttgtgaa
    11821 tctccaggcc tgataagacg cggtaagcgt cgcatcaggc attggttctt acctctgctc
    11881 ccccatcatc tcccgcacca gctccacgca gcgcagaaaa cggctgtcgt aatcgtcctc
    11941 ttcaacccgc acgaattcga tattgttctc ttccagcatc tccaccagca agttctggaa
    12001 ctctttgcga tccaccgaac tgccgaggct gcgtaaacca tccgccaccc acggcgtgtt
    12061 gttctccagc aggatcacca gatcgaaacg gtattcatca atcaacgcct gcacaaacgg
    12121 atgctcacgc ccttcgtact ttttgcagaa cgcctgggta gtgacaaaat cggtgtcgat
    12181 aaacgccact ttattggcat atttcactgc aaaatcaata tattgcgcat gtcccagcgc
    12241 gattttatcg tagtcggaat actgcaacgc gatctcatcg ccaccgaggt gcgaaaagac
    12301 ataatcacga ccatattccc acgcactggt agtgttgaag atattggcaa gtttgtttac
    12361 cagggtggat ttaccgctcg attcaccacc gaggatcgcc acggtacgca caaagaatgg
    12421 cttcacttcc gtaggaatat attcccagta gcggaacggg ttttcgcgga tctgcgcacc
    12481 gctgatactc ataaaggtac gcttcggatc gaccagcacc gtctcgatcc ccagatgttc
    12541 catatactgt ggcgcatcgg cttcttccga ggtgtagatc aggtccggct gaatcccttt
    12601 ttcagccata aactttttga tgccgttgct ccatacatcc cagccgtgtg gatatggctc
    12661 catgccctct tcattgaaag catgaatacg gatatttttc tgatatttaa aggtttgcaa
    12721 tagccagcgc agacgatccg gcaccgtggg ctgctgcgac atggcgctgt cttcgaacaa
    12781 tgcgcggtcg cgggtatcgt caaaacccat aatgatatgc agctcgtcaa cctggctaca
    12841 ggcgcgctgg ataaggtaga tatgtccggt atgcagcggg taaaacttac cgaatacgac
    12901 accgatcgtt ttcttctgcc ggggaaattc aagtcccaaa taacggtgca atgcctccag
    12961 cttttgtgcg ctggggcttt taattttggc attcagtaac tggcttaaat accctttggt
    13021 cataccgctg gcatcagcta cctgctgtag cgtgcagccc tgttgcttga tggcagtttt
    13081 caggtaatca aatgacgaca tatctccctc cgtatctctc attataagtc gtcgaacacg
    13141 ctaagcgcgt cggagagttt tttaacgcca aaaatctgca tcccttccgg cgtttttttc
    13201 ggcacgttag ccgccggaac aatcgcccgg cgaaaaccgt gtttcgccgc ttctgagatt
    13261 cgttcctggc cgctgggcac cgggcggatc tcccctgcca gcccgacttc accaaacacc
    13321 accagatcct gcggtagcgg tctgtcacgc aggctggaaa ccatcgccag cagtaacgct
    13381 aaatcggcac tggtttccgt caccttcacg ccaccgacca cgttaacaaa cacatcctga
    13441 tcggccattt gtagaccacc gtgacggtga agcaccgcca gcaggattgc cagacggttt
    13501 tgctccagcc cgactgccac gcggcgtgga ttcgccatca tcgagtgatc gacgagcgcc
    13561 tgaatctcca ccagcagcgg acgcgttcct tcccacacta ccatcactga gctaccggag
    13621 gtcacttcat cgccgcgact taagaaaatt gccgaagggt tgctgacttc acgcagcccc
    13681 tgctcggtca ttgcaaagac acccagctca ttcaccgcgc cgaagcggtt tttatggctg
    13741 cgtaaggtgc gaaaacggga gtcggcatcg ccatccaaaa gcaccgaaca gtcgatacag
    13801 tgttccagca ctttagggcc agccagcgag ccatctttgg ttacgtgccc aaccatgaca
    13861 atcgccacac cgcgcgtttt ggcgaagcgc gtcagataag ccgccgtttc acgcacctgt
    13921 gccacgctgc caggcgatga ttgtacatcc gccatatgca tcacctggat ggagtcaatt
    13981 accatcagct tcggttgctc ttcttcggca atcaggcaga tctgctcgat gctggtttcc
    14041 gacagcatat taagattgtc agtcggcagg ccaaggcgat gagcgcgcat tgccacctgt
    14101 tgcagcgact cttcgccggt gacatacagc gttttcatct gctgcgccag tttgcacagc
    14161 gtttgcagca acagcgtgga tttccccgca ccagggttac cgccaatcag aatggcgctt
    14221 cccggcacca cgccgccgcc taatacgcgg tcgaactctt taaatccggt ggaaaaacgc
    14281 ggcagctctt caaggctgat gtcggaaagt ttctggactt ttgccactcc ggcgctaccg
    14341 gcatagccac tgagacgctc gttacgcgcc accgttggcg acgctgcaag acgcacctcg
    14401 gtgatggtgt tccaggcatg acaggcactg cactgcccct gccagcgcgg ataatcggcc
    14461 ccgcattcat tacaaacaaa ggcgcgtttt ggagcttttg ccacgatgta cctcgttaat
    14521 gctgtgccgc ccgcaggatg acgggcggac aattacttct gattcaggct gccagagagg
    14581 atgcagaata cccccatcag gtcagcgtga cggatggtga cttccgtctt ttcattcact
    14641 tttggcttgg catggtaggc aatccccagc cctgccgctt tgatcatcgg caggtcattg
    14701 gctccatcgc caatcgccac ggtctgcgcc agcgggattt catactcctg cgcgaggcgg
    14761 gtcagggttt tcgctttata ttgcgcgtca acgatgtcgc cgatcacatt gccggtaaat
    14821 ttaccgtcca tgatctccag ttcattggct accacggctg tcaggcgcag cttgtcgcgc
    14881 aggtattcag caaagaaagt aaagccgccg gaggcaatcg ccactttcca gcccagcgtt
    14941 tccagcttga gtaccagctg cgttaagcct gacatcagcg gcagattttc acgcacctgt
    15001 tgcagaatat tggcgtcagc gcctttcagc gtcgccacgc ggctgcgcag gctggcggta
    15061 aaatcgagtt cgccgcgcat cgcccgttcg gttacctccg ccaccctctc gcccgttccg
    15121 gccagtttgg caatttcatc aatacattca atctggatgg cggtggaatc catgtccatc
    15181 accagcaaac ccggcgtgcg caggtgtggg attttcccca gcggtgcgac atccagttgc
    15241 gcttcatggg ccaggcgcgt agcccgcgca gtgagtgaac ctgccagacg aatcacctga
    15301 taatcttcca cgcaccaggc ggcaacaatc accatagccg cgcccagttt gctctggtat
    15361 tgggtcagac gttgtttatc cagcccacga ccatacagca gccagccgct acgacctgcg
    15421 tggtaatcca gtggcatcac ttcatcacca cttaatgaaa gaggcagacc aggccataaa
    15481 gagacatctt caggcaagtc gcaccaggta atgttaggca ttaaggctcc tgtaaaatcg
    15541 ttcgaagcag ggaaaataac gcatgaggct accttgtatc cattgcttct ggcaacatta
    15601 agtctcaaat tttcaaaggg tggaagatgg ctcgcacaaa actgaaattc cggctgcatc
    15661 gggcagtgat tgtcctgttc tgtcttgcct tgttagtggc gctgatgcag ggggcgtcat
    15721 ggtttagtca aaaccaccag cgacagcgta atccgcaact ggaagaactg gcccgcaccc
    15781 tggcgcggca ggtgacgcta aacgttgcac cgctgatgcg taccgactca ccggatgaaa
    15841 aacgcattca ggcgatcctc gatcagttaa cggatgaaag ccgcattctc gacgcaggcg
    15901 tatatgatga acaaggcgat cttatcgccc gttctggcga aagcgtcgac gtgcgcgacc
    15961 ggctggcgct cgacggcaaa aaagcgggcg gctattttaa tcagcagatt gtcgaaccca
    16021 ttgcgggtaa aaacggaccg ctcggctatc tgcgcctgac gctcgacacc catacgctcg
    16081 ccaccgaagc ccaacaggtg gataacacca ctaacatttt acgcctgatg ttgctgctct
    16141 cgctggcaat cggcgtagtg ctgacccgca cgctgctaca gggtaaacgc acccgctggc
    16201 agcaatcgcc cttcctgtta accgccagca aaccggtgcc ggaagaggaa gaaagcgaga
    16261 aaaaagagtg acccattact acaagaaagg aaatcgttat gtccacatta cgcctgctca
    16321 tctctgactc ttacgacccg tggtttaacc tggcggtaga ggagtgtatt tttcgccaga
    16381 tgcccgccac gcagcgcgtt ctgtttctct ggcgcaatgc cgacacggta gtgattggtc
    16441 gtgcgcagaa cccgtggaaa gagtgtaata cccggcggat ggaagaagat aacgtccgcc
    16501 tggcgcggcg cagtagcggt ggcggcgcgg tgttccacga tctcggcaat acctgtttta
    16561 cctttatggc tggcaagccg gagtacgata aaactatctc cacgtcgatt gtgctcaatg
    16621 cgctgaacgc gcttggcgtc agcgccgaag cttccgggcg taacgatctg gtggtgaaaa
    16681 ccgccgaagg cgaccgcaaa gtctcaggct cggcctatcg cgaaaccaaa gatcgtggct
    16741 tccaccacgg cactttgctg ctcaatgccg accttagccg cctggcaaac tatctcaatc
    16801 cggataaaaa gaaactggcg gcgaaaggca ttacctcagt gcgttcccgc gtgaccaatc
    16861 tgaccgaatt gctaccgggg atcacccatg agcaggtttg cgaggccata accgaggcct
    16921 ttttcgccca ttatggcgag cgcgtggaag cggaaatcat ctccccggac aaaacgccag
    16981 acttgccaaa cttcgccgaa acctttgccc gccagagtag ctgggaatgg aacttcggtc
    17041 aggctccggc attctcgcat ctgctggatg aacgctttac ctggggcggc gtggaactgc
    17101 atttcgacgt tgaaaaaggc catatcaccc gcgcccaggt gtttaccgac agtctcaacc
    17161 ccgcgccgct ggaagccctc gccgggcgac tgcaaggctg tctgtaccgc gcggatatgc
    17221 tgcaacagga gtgcgaagcg ctgctggttg acttccagga acaggaaaaa gagctacggg
    17281 agttgtcggc atggatggca ggggcggtgc ggtagttacc cacccatgcg ggtaactttc
    17341 tcttcgattt gccggatttt ttccggcatt tcttgcgcaa tgtggcgaaa cgcctcgctg
    17401 atacgcggat ctctggcgac cgtttgccag aactgctgcg ccagcgggat cgccgttaac
    17461 catgcgcttt tactgatgtt aagatcaaac ttcacctcaa tggcagcatc acgcagcatt
    17521 ccggcgctgt ttggcgcata ggccatcggc agcatgtcgt agacgggtgt cagcgcaaat
    17581 ggtggttcag ataaataaaa tgataaattg cctgcgtgca tatcgctgtt ggcgataagt
    17641 cgcccaaacg cccagatcac ttctgtttgc gccacgctct ggtgagtgac aagttgttgc
    17701 tcacacaaat gccgcattgc ctccggccac gatcccggag aagagataaa ctcactttgc
    17761 accgcctcca gcgacacaat gggcaagcga ccatcgttac ctttgcagtc aaagcgttcc
    17821 gcttccagga atacctgcct gttacttgtt acaagcaccg ttgactcgat ggcgtggatc
    17881 ccgccgtcac gcaggatttg cgcggcaata gattcagcaa ttagcaggtc cccccaacgt
    17941 tggctgactg cggtttgctg tggcacggtg aatttcacca acacatgttt attgcctgac
    18001 ggcgtttgtg catagcaggt aaattttggc tgctcgccgc ccgcagaaga acccacaatt
    18061 tctccagcca gagcatcgct tgccaactgt tcgtaacggg tgagtttttg atccaaagga
    18121 atttccgcag ggcgttgtgc agtaatccat cgctgataat tcccctcccc gaccaaccaa
    18181 ccgccagtat attcaccgtt aaatacggtc agggcgtaga gcacatcttc ttcctgccag
    18241 agacgtatat catcagtcag attcagttgc gcggctaact ttctgcccca cgcgcgccct
    18301 aaaaagccct gcggtcggag atcggtcaaa taccagggca aaccatcaaa ccactgttcg
    18361 tcgccatctg cgcctgttac cagacaactt ccctgtggcc agcacaaccg gatgtcggcg
    18421 aatttatgcg cctttccggc atcgtccacc cgccagacgg gaatacgctc aattccgcga
    18481 taaggacgca gcagtacata tcgcgttgcc cgtgctttac caaagcgaat cacccgattt
    18541 tctctggcaa caaggcgtga gaacgtcgcc tgactgattg ccagacgctg gcgcaattcc
    18601 ggggcagaac gcggcccttg cagtaaaaga tcggtcagtt cgctcattaa tgaatagatt
    18661 cgtgaataga tacttgaata gatttattaa catatttcac tgaaaagtat agcgaaaata
    18721 ttcaagctgg agagataaat gaataaatac taaaaagccg gagcagtctc ccactccggc
    18781 ttcacacgac aatcgccttt cagcaaaaca caattactct ttatcgccca gcagaacgga
    18841 ttccagcgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg cagtggtctg
    18901 ctcgtgagtg cggatgtggt cagacacggt gcagatggtc agggctttcg cgccaaattc
    18961 tgcagcgacg ccgtagatac ccgccgcttc catttccacg ccgaggatgc cgtatttttc
    19021 catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga acaggttacc
    19081 aacgcgagca tcaacgccca gtgcttttgc cgcgtctacc gcgttacgca ccatgtcgaa
    19141 gtcagcgata gcggcaaagt catggtcttt aaaacggatg cggttaactt tggaatcggt
    19201 gcaggcaccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa cagcgccaca
    19261 ggaacccacg cggataattt tcttcacgcc gaaatcagtg atcagttctt tggtgtagat
    19321 ggagcaggac gggataccca taccgtggcc cattacggaa attttgcggc ctttgtaagt
    19381 accagtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt caaggaaagt
    19441 ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt cagcaaaatc
    19501 gcccatttct gcattaatgt gtggagtagc catcgtttta tcctttttgt gacatattaa
    19561 aggcggagtc actgctccgc ccaaatccat cagaacatgg ctttgccata ttccatatca
    19621 gaagtaccaa aatattttgc cagagtctgg ccgatatccg cgaaggtttc acgatgaccc
    19681 agcgaacctg gtttcacttt cgggccgtaa accagtaccg gaatgtgttc acgcgtgtgg
    19741 tcagtaccgg tccaggtcgg gtcacaaccg tggtcagcag tgagaatcag aatatcgtca
    19801 tcgcgcagca aagacatcag ctccggcaga cggcggtcga acagttccag acccgcagca
    19861 taaccggcga cgtcgcggcg gtggccccag gaagagtcga agtcaacgaa gttggtgaag
    19921 acgatggtgt tatcacccgc ttctttcatc tctttgatgg tggcgtcaaa cagcgcgtcc
    19981 agcccggtcg ctttcacttt tttggtgata ccgcagttgg catagatgtc tgcaatttta
    20041 ccgaccgaaa ccacctggcc gtgtttttca tcaaccagtt tctgcagcac ggtcggtgct
    20101 ggcggctcaa cagccaggtc gtgacggtta ccagtacgct ggaagttacc ggctttgtcg
    20161 ccgataaacg gacgagcgat aacacgaccg atgttgtagc cgccgttggt cagctcttca
    20221 cgggcgattt cgcacagctc gtagagttta tccaggccaa aggtttcttc atggcaggca
    20281 atctggaaca cggagtcagc ggaggtatag aaaatcggct tgccggtttt catatgctct
    20341 tcgcccagtt gatccagaat gaccgtaccg gaagagtggc agttaccgag gtaacccggc
    20401 agattagcgc gttcgaccag tttatccagc aactcttgcg ggaagctgtt ttcgtgatcg
    20461 gagaagtatc cccactcaaa cagaaccggc acaccagcaa tttcccagtg accagacggg
    20521 gtgtctttac cggatgacat ttcgtgcgcc catgcgtacg cgccgataac ttcagcgttg
    20581 ccatccattc ccgccggaat gaaaccggta gaaccttcgt gtgctttcgc cagcccaaga
    20641 cgggtcagat ttggcagatt gagcgggcct ttacgaccgt ggtcagcttc gcctttggca
    20701 caagcttctg cgatatgacc cagggtgtca gccccgacgt caccaaagcg ttctgcatct
    20761 tctgtagcgc cgatgccgaa tgagtccagc accataataa atgcacgttt catatgttct
    20821 ccgtactcag tgcttcaaat ttaaatgatc ggatcagtat accgttattc gctgatgcgg
    20881 cgatagacag ttggtgtgct ttccggtgct ttatcggcaa gtttaattgc cgctttcacc
    20941 gctttcgccg cttcctgcca gctgttttcg tctttcgcgt ggataactgc cagcggacgc
    21001 tgaccgtcta cctggtcgcc cagacgcgcc atatcagtaa agccgacgct gtaatcgatg
    21061 gtgtcagatg cctgacggcg tccgccgccc attgcaacca ctgccatccc cagtgcgcgg
    21121 gtatccattt cactgacaaa cccttcggta tcagcataga ctgctttcgt cagcatcgct
    21181 gtcggcagat acttcgcgta gttctcaacg aagtcggtcg ggcctttttg tgccgcgacc
    21241 atacgaccaa agacttctgc cgctttaccg ttgtccagca ccgcctgcaa tttcgcgcgc
    21301 gcttcggcgt catttttcgc cagtttgccg gagatcagca tctccacgca cagtgccatc
    21361 gtgacatcaa acagacgcgg gttacgatgt tcacccgtca ggaactgcac cgcttcacga
    21421 acttcaaccg cgttacctgc actggaggcc agtacctgat tcatatcggt gagcagcgcg
    21481 gtggtgcgca cgccagcgcc gttagccacg ccaacaatcg cttcggcaag ggcttcagag
    21541 agttcgtagg tcggcataaa cgcgccgcta cccactttca cgtccatcac cagcgcgtcc
    21601 agaccttccg ccagtttctt cgccagaata gaggcggtga tcagcgggat tgaatccacg
    21661 gttgcggtaa tatcgcgggt cgcgtagaaa cgtttatccg ccggagccag tgagctggtc
    21721 tggccgataa tcgccacgcc gacgtcttta ataatttcgc ggaaacggtt gtcatccggg
    21781 aaaatgtcga agccagggat ggattccagt ttgtcgagcg taccgccagt atgaccgagg
    21841 ccgcgaccgg agatcatcgg aatatagccg ccacaggcag cgaccattgg ccccaacatc
    21901 agcgaagtca catcgccgac accgccggtc gagtgtttat caacaatcgg gccattcaga
    21961 tgcaggcttt tccagtcgag aacggttcct gaatctcgca tcgccatggt cagcgagaca
    22021 cgctcaggca ttgtcatatc gtggaagaaa atggtcatcg cgagggcggc aatctgccct
    22081 tcggagatag tgttgtcgcg aatgccgttg ataaagaaac gaatttcttc atcactcagc
    22141 gcatgaccat cacgtttttt acgaataatt tcttgtgcga gaaacaaggt aacctcccgt
    22201 ggaaaagaga cgaggtaatc ggggaaccgg atgagcatcg ctgcatcatc cggcaaatca
    22261 agccttagta gctgctggcg ctcttaccgt cgccgtggcc cagagctttc agcaggcttg
    22321 ccagcaagct ggaagcacca aagcggtagt gacgcgcatc agcccagtca gcaccgaaca
    22381 gttcatctgc aatggcgaga tatttctgcg catcttccgc agtacgcacg ccgcctgccg
    22441 gtttgaaacc aacggttttt tctacgccca tatcgcggat cacttccatc atgatgcgcg
    22501 cgctttccgg cgtcgcattc acagccactt taccggtaga ggttttgatg aagtcagcac
    22561 ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttc agttcgccgg
    22621 tttcaataat cactttcagc agtacgttcg ccgcagcgca agcctcttta caggctttca
    22681 ccaggtcaaa accaacctgc tcgttacctg ccatcagcgc gcggtacggg aacaccacgt
    22741 caacttcatc ggcaccgtag gcgattgccg cacgggtttc tgccagcgcg atttcgatgt
    22801 cgtcgttacc gtgtgggaag ttagttaccg tagcgatacg gatttccggg gtgccctgct
    22861 ctttcagagt tttgcgagca atcgggataa agcgaggata gatacagata gcggcggtat
    22921 tgccgaccgg agttttggcc tgatgacaca gtgcgatcac tttctcatcg gtgtcgtcgt
    22981 cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct ttcagatcag
    23041 tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg ttagtattct
    23101 aacatctact ccgcaacaca cttcgatgta catcacaatt aaggaaatct acttacaagt
    23161 ttgcatcact gtaatgcgat ctggttcaaa taattcactt tcaaatgaat gcgtcagtgg
    23221 tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg cgcatcacct
    23281 tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca acaacgttgc
    23341 cagcaaattg tcaccagccc ggtacttagc ccggagcaga agcgccattt tctggcactg
    23401 gaagcagaaa acaatctgcc ttacccacag ctgcctgccg aagcccgccg cgcgctggat
    23461 gagggggtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg ctatgtctta
    23521 cccgattacg ctcgttttct ggcgcacggt tccgaatggc tggagctgga aggcgcgaaa
    23581 gaccttgatg acgcactctc tctgctgacc attctttacc accatgtacc gtcggtcaca
    23641 tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt tagaattcta
    23701 acacaagacg agatcgatat tcgaataaaa cgtttctggc gttacctcga cagaaccctg
    23761 ccagacgcct ttatgcatgc caatatcggt ccgtctgatt cgcccattac ccgtgcaata
    23821 ttacgtgcag acgcagagct gaagcaggtt tcaccgaacc tgacctttat ctacgatcct
    23881 gatatcaccc ctgatgacct gctgctggaa gtggcgaaaa acgtctgtga atgtagcaaa
    23941 ccgcacatcg ccaacggtcc ggtgcatgat aaaattttca caaaaggggg ctacgggatt
    24001 gtgagctgtt acaactcact gccgctggcg ggtggtggca gcacgctggt acgccttaac
    24061 ctgaaagcca ttgccgagcg cagcgaatcg ctggatgact tctttacgcg cactctaccg
    24121 cactactgcc aacagcagat cgccatcatc gatgcgcggt gtgaattcct ctatcaacaa
    24181 tcacacttct ttgagaatag cttcctggtg aaagaagggt tgattgatcc tgaacgtttt
    24241 gtgccgatgt ttggcatgta tgggctggcg gaagcggtta acttgctgtg tgaaaaagaa
    24301 gggattgccg cacgctacgg taaagaggcc gccgcaaatg aagtgggtta tcgcattagc
    24361 gcgcaactgg cggagtttgt cgccaatacc cccgtgaaat atggctggca aaaacgcgcc
    24421 atgttacacg cacagtcggg gatcagttcc gatatcggca ccacgccggg cgcgcgtttg
    24481 ccatatggtg atgagccaga tccgattacc catctgcaaa ctgtcgcccc gcatcatgct
    24541 tattattatt ccggcatcag cgacattctg acgctcgacg aaaccatcaa acgtaatccg
    24601 caggcgctgg tacagctttg cctcggtgcc tttaaagccg gaatgcgtga atttaccgcc
    24661 aatgtcagcg gtaacgatct ggttcgcgtt accggttata tggtgcgttt gtcggattta
    24721 gaaaaatatc gcgccgaagg ttcacgcacc aacaccacct ggctgggaga agaagccgca
    24781 cgcaacactc gtattctgga acgccagccg cgcgtgataa gccatgaaca gcagatgcgc
    24841 tttagtcagt aagattatcc ccttctcctg cgttgacggg ccaggcagtc gtctggctct
    24901 gtttttgcag ggctgcaatc tgcgctgcaa aaactgtcac aatccgtgga cgatgggacg
    24961 ttgcaatgac tgtggggagt gcgtgccaca gtgtccgcat caggcgttgc agattgttga
    25021 cggcaaagtg gtgtggaacg ctgtggtttg cgagcagtgt gatacctgcc tgaagaggtg
    25081 tccgcaacat gccacgccaa tggcgcaatc catgagcgtg gacgaagtgc ttagccatgt
    25141 ccgcaaagcg gtgctgttta ttgaagggat aacggtaagc agcggcgaag ccacgaccca
    25201 actgccgttt gtagtggcgc tgtttactgc tatcaaaaac gatccgcaac tgcgccacct
    25261 cacctgcctg gtggacagta acggtatgtt gagcgaaacc ggctgggaaa aattactccc
    25321 ggtgtgcgac ggcgcaatgc tcgatctcaa agcgtggggg agcgaatgtc atcagcaatt
    25381 aaccggacgc gataatcagc agattaagcg cagcatctgt ttgctggcag agcgcggtaa
    25441 gctggcggaa ctgcgtttgc tggtgatccc tggccaggtg gattatttgc aacacatcga
    25501 agaactggcg gcgtttatca agggactcgg cgatgttccg gtacgcctga acgcgtttca
    25561 tgcccacggc gtgtatggcg aggcgcaaag ctgggcgagc gccacgccgg aagacgttga
    25621 gccgttggct gatgcgttaa aggtgcgcgg ggtgagccgg ttgatatttc cggcgctcta
    25681 tttgtgacat gccggatacg gcctacggtt cggcacagac ttgtaggcat gataagacgc
    25741 gacaagcgtc gcatcaggca ttatgcgccg actgccggat gcggcgtgaa cgccttatcc
    25801 ggcctacggt tcggcacaga cttgtaggca tgataagacg cgtcagcgtc gcatcaggca
    25861 ttgtgcgcca actgccggat gcggcgtgaa cgccttatcc ggcctacggt tcggcacaga
    25921 c
//
LOCUS       DACADF010000065        25811 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.065, whole genome shotgun sequence.
ACCESSION   DACADF010000065 DACADF010000000
VERSION     DACADF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.065"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(166..813)
                     /gene="ychE"
                     /locus_tag="ISB30_002480"
     CDS             complement(166..813)
                     /gene="ychE"
                     /locus_tag="ISB30_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAP1990106.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1290..3965
                     /gene="adhE"
                     /locus_tag="ISB30_002481"
                     /gene_synonym="adhC"
     CDS             1290..3965
                     /gene="adhE"
                     /locus_tag="ISB30_002481"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP1990107.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4090..5137
                     /locus_tag="ISB30_002482"
                     /pseudo
     CDS             4090..5137
                     /locus_tag="ISB30_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5423..6040)
                     /gene="tdk"
                     /locus_tag="ISB30_002483"
     CDS             complement(5423..6040)
                     /gene="tdk"
                     /locus_tag="ISB30_002483"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP1990108.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQAYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            6644..7057
                     /gene="hns"
                     /locus_tag="ISB30_002484"
     CDS             6644..7057
                     /gene="hns"
                     /locus_tag="ISB30_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAP1990109.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7202..8110)
                     /gene="galU"
                     /locus_tag="ISB30_002485"
     CDS             complement(7202..8110)
                     /gene="galU"
                     /locus_tag="ISB30_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP1990110.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8312..9325)
                     /gene="rssB"
                     /locus_tag="ISB30_002486"
     CDS             complement(8312..9325)
                     /gene="rssB"
                     /locus_tag="ISB30_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAP1990111.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISQCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9417..10322)
                     /gene="rssA"
                     /locus_tag="ISB30_002487"
     CDS             complement(9417..10322)
                     /gene="rssA"
                     /locus_tag="ISB30_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAP1990112.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10435..10893
                     /locus_tag="ISB30_002488"
     CDS             10435..10893
                     /locus_tag="ISB30_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAP1990113.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDGGDIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10943..11785
                     /gene="purU"
                     /locus_tag="ISB30_002489"
     CDS             10943..11785
                     /gene="purU"
                     /locus_tag="ISB30_002489"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAP1990114.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDNLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11945..12029
                     /locus_tag="ISB30_002490"
     tRNA            11945..12029
                     /locus_tag="ISB30_002490"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11979..11981,aa:Tyr,seq:gta)
     gene            complement(12046..13137)
                     /locus_tag="ISB30_002491"
     CDS             complement(12046..13137)
                     /locus_tag="ISB30_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HAP1990115.1"
                     /translation="MNLFNPDRLITFPADDPQLVKQLQSGTKVYLAKSGDHRYADGWA
                     FVKMAVLIIACFSCYLLALFQRQWGLYLLWYLAMMFCAMLLAVNVVHDASHDAFLRGK
                     KTNARLNRWVAFPMGLDPDCWRVRHVRFHHGFTNIEFYDPDTAENGILRQTPWQRWRP
                     FMRQQHRYWPLVAALTFPWYIWVVDWLDRAGFTPVTRHLALRGFAGWGYFLAGKLAHC
                     ALCLIIPWLLSDFSFMTILLTYLLSQLLASLIFVTLIIGTHWAKGHTQLPPEEGKMPV
                     GRLAHTFATTFDWTPQPAWLGYWLGGINLHLTHHLFPHWHHRHYSALSRIIAQIASQQ
                     GLDYQLLTLADLLRLQQQFLRRMGEKPKV"
     gene            complement(13124..14248)
                     /locus_tag="ISB30_002492"
     CDS             complement(13124..14248)
                     /locus_tag="ISB30_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HAP1990116.1"
                     /translation="MSDLLFPIISMVLLVLAEAGVLQFTGRQKVDWLDVIFNINSGHM
                     MLWLFRCLEVLCYGLVVNHFSLGLFDNVPVILLWLFTILAWDFGFYWLHRWHHEMRPL
                     WAVHVVHHQGEHYNLSLGVRNSWYSSLTSIPFFMLLAMLGVPLQVFLAVSIIHYSWQF
                     FNHNALTPKLGWLEKVLITPSHHRVHHLNQKQYADTNYGGTFLLWDKLFGSYSAGPEY
                     DVGYGVKNSRLSCNPMRESNLPFLRLMGFCSFGKAIEGRYQSSPWAVGAGVILLFMLV
                     LGYIQQYGYGIEFITSEQAVLFVLLAAGSVALGGMSDGQHWGMILWCSVTLSLLYFIV
                     YMCSWSTLLWKGLVGILLAHCLLFVIGVGRKSLRKQYEPV"
     gene            complement(14241..14843)
                     /locus_tag="ISB30_002493"
     CDS             complement(14241..14843)
                     /locus_tag="ISB30_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HAP1990117.1"
                     /translation="MSNSLLRLKQMLLGWGTVGVIYNFSDYLQGEGYQLTPSIIDNLI
                     AFSPSAVWLYLSFFFIVPLGYLSTPLCHLRWLARSMQISALVAGVFYLLWPTTMHYPS
                     FDQMGISAGALNRLIAIDSSQNCFPSLHAALTLLAVWAIAKKGNRWLTLMSVVWAIAI
                     AFSILQLRRHLFIDLVGGALLASICGWICATCEKRKTQYE"
     gene            complement(14840..16126)
                     /locus_tag="ISB30_002494"
     CDS             complement(14840..16126)
                     /locus_tag="ISB30_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HAP1990118.1"
                     /translation="MIPLMLIWRYFRTRRLHFTDREALENWQAKRLHQFRQNVLSHSP
                     WFQRYLTLPFNQWPMMDKALMMTHFDEMNTAGLKRDELLDCAMRSEQSRDFKPCVGKF
                     SVGLSSGTSGRRGLFVVSPHEQQMWAAGVLAKVLPDDLFAKERVALFLRADNNLYQSV
                     NNRWLSLDFYDLLAPFQAQLKLLQQRAPTIIVAPAQVLRALALEVMAGELTLKVKKVI
                     SVAEVLEPQDRELLRNVFHNVGEIYQATEGFLASTCRCGTLHLNEEFVHIEPQWLDEH
                     RFVPIVTDFTRTTQPIVRYRLDDVLVASEQPCPCGSATMAIARIEGRQDDQLQLPTRS
                     GDIQTVFADACSRVLAMTLPLTVDYRLLQTGSAQLTLIADCELWMLEHCREALNALFI
                     RQDIAVGQLIWSLELQTVPVSFTAKRRRIVRQWGRE"
     gene            complement(16123..16929)
                     /locus_tag="ISB30_002495"
     CDS             complement(16123..16929)
                     /locus_tag="ISB30_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP1990119.1"
                     /translation="MAKITAFETGWCTHISCIALKGAGLKVCKFPARAWLLEVGDKRW
                     LWDTGYSSWFEHYTQSGVFRLYRQVTPVYFDPQQALVRQLSATGLAAKDVDAIILSHF
                     HADHIAGLRDFPDVSCICSGEGWRQVRTLRGVTALRQAFVPGLIPENFDSSLRFIESF
                     ASVNLPAQLAPFECGYLLPESNGEIILVPLPGHAAGHIGAFVLTDNGWTLLAGDAAWA
                     PANYRELRGPSPLAHLVMSDSHAYYHTLGQLHQLWQNGVVEIRLCHEGDL"
     gene            complement(16907..17923)
                     /locus_tag="ISB30_002496"
     CDS             complement(16907..17923)
                     /locus_tag="ISB30_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP1990120.1"
                     /translation="MKILVTGATSGLGRNAVQYLLQSGESVVATGRNCEVGRSLTNAG
                     AQFVALDLTEATEDDCARLMDGCDAVWHCAAKSSPWGDKTAFWLANVKVTHTLAQAAG
                     RLNIPRFIHISTPAVYFDFQHHYDLPETYLARAFSSHYASSKYAAEQVIAGAVSRYSA
                     TRFILLRPRGLFGPHDQVIVPRLMQQLQQGGGCLRLPRGGDALLDLTFVQNVVYAMRL
                     ATNVEGLRSGSIYNISNHQPQQLSVMLDALLHQQLGLSYRIAAVPWSLLSLIARGLEL
                     YGKCVNQEPVITRYSAGTLCFDMTLSAQKAITELGYRPRFSMEQGIAITGQWLREHGK
                     NNRI"
     gene            complement(17920..18927)
                     /locus_tag="ISB30_002497"
     CDS             complement(17920..18927)
                     /locus_tag="ISB30_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP1990121.1"
                     /translation="MARPNQPTISAALKIIATGIALPPDQITSKQLDVQLNKPVGYVE
                     RRSGICWRYFARNDASQAELAAEALHNALVAAALNPSDIDLLICASAIAVQALPCSAI
                     HILKAAAMPVGVAGFDINSSCVSFISALEVAAGLLNCGAYRRIAIVSADIASRGIDWD
                     HEESSLIFGDGAACAIVERGDGRSGIIASLIEMYPEGSELCEIRAGGTRRNPRSGVSD
                     SDFLFHMQGKPLFRFASSLIEDYFSRLLQKSSLQLSDIGTVVPHQASHLSLEHMRKRL
                     RVPESALIDVYRFYGNQVAASIPTALHEAVISGRFTADKPVMLIGTAAGLTLAGMVLL
                     P"
     gene            complement(19055..19732)
                     /gene="narI"
                     /locus_tag="ISB30_002498"
     CDS             complement(19055..19732)
                     /gene="narI"
                     /locus_tag="ISB30_002498"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAP1990122.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(19732..20442)
                     /gene="narJ"
                     /locus_tag="ISB30_002499"
     CDS             complement(19732..20442)
                     /gene="narJ"
                     /locus_tag="ISB30_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAP1990123.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(20439..21977)
                     /gene="narH"
                     /locus_tag="ISB30_002500"
     CDS             complement(20439..21977)
                     /gene="narH"
                     /locus_tag="ISB30_002500"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAP1990124.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(21974..25717)
                     /locus_tag="ISB30_002501"
     CDS             complement(21974..25717)
                     /locus_tag="ISB30_002501"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAP1990125.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         6636 a         6848 c         6216 g         6111 t
ORIGIN      
        1 taacatgatg gtgaggagaa gcagttaagg aaatccttat aactaactgt aatttgagaa
       61 gaatattttt acctattcct tttgcttttg ataactgcgc aggaacaaag taaaaataat
      121 gacgaagtaa acggagcatg gcagctccgt ttcatttgaa aggaattaat taagcaggcc
      181 ggggaaaatc cccttaatac cagtaacgat aaattcaatc cccaacgcca tcagcaatag
      241 ccccataata cgcgtaatca cgttgatgcc ggtctggcgt aaaactcgta ccagccacgg
      301 tgccatgcga aacaatcccc agcaacataa agcgaacaat gcaatagcca caaagaaacc
      361 aaacagatag ctaatgctgt gataacgcgt accccagacg atggtagaac tgatcgcccc
      421 tggccccgcc atcaacggca acgccaatgg caccacacca atgctttcac gcaccgcggt
      481 ttctgatttt tcttgcttgt tctgtttatc ctcaccaagc ttgccgctga tcatcgacat
      541 cgctattgtc accaccagga tgccaccggc gatacggaat gaatcaattg atataccaaa
      601 aagttgtaga atcgtgtcgc cgagaaaaag cgagatccac aagataatgg ccacagacag
      661 gttggctgta aggttagttt tgtttcgcgc tgccgctgtc tgataactgg tcatgctgat
      721 aaagacggga ataatcccta ccgggttgac cagcgcaaat aacccgatga aaaatttgaa
      781 gtaaacggga aaatcaaaaa aggtctgaat cacggttagc tccgaagcaa aagccggata
      841 atgttagcca taaataaggt tgaaaagacg cgctgacaat acgccttttg acagcatttt
      901 tcacctccta actacttaaa attgctatca ttcgttattg ttatctagtt gtgcaaaaca
      961 tgctaatgta gccaacaaat catactacaa tttgttaact gttagctata atggcgaaaa
     1021 gccatgctga aaggtgtcag ctttgcaaaa atttgatctt gatcacgtaa tcagtaccca
     1081 gaagtgagta atcttgctta cgccacctgg aagtgacgca ttagagataa taactctaat
     1141 gtttaaactc ttttagtaaa tcacagtgag tgtgagcgcg agtaagcttt tgatttccat
     1201 cggttaagca aatcatcacc gcactgacta tactctcgta ttcgagcaga tgatttacta
     1261 aaaaagttta acattatcag gagagcatta tggctgttac taatgtcgct gaacttaacg
     1321 cactcgtaga gcgtgtaaaa aaagcccagc gtgaatatgc cagtttcact caagagcaag
     1381 tagacaaaat cttccgcgcc gccgctctgg ctgctgcaga tgctcgaatc ccactcgcga
     1441 aaatggccgt tgccgaatcc ggcatgggta tcgtcgaaga taaagtgatc aaaaaccact
     1501 ttgcttctga atatatctac aacgcctata aagatgaaaa aacctgtggt gttctgtctg
     1561 aagacgacac ttttggtacc attactatcg ctgaacccat cggtattatt tgcggcatcg
     1621 ttccgaccac taacccgact tcaactgcta tcttcaaatc actgatcagc ctgaagaccc
     1681 gtaacgccat tatcttctcc ccgcacccgc gtgcaaaaga tgcaaccaac aaagcggctg
     1741 atatcgttct gcaggctgct atcgctgccg gtgctccgaa agatctgatc ggctggatcg
     1801 atcaaccttc tgttgagctg tctaacgcac tgatgcacca cccagacatc aacctgatcc
     1861 tcgcgactgg tggtccgggc atggttaaag ccgcatacag ctccggtaaa ccagctatcg
     1921 gcgtaggcgc gggcaacact ccagttgtta tcgatgaaac tgctgatatc aaacgtgcag
     1981 ttgcatctgt actgatgtcc aaaaccttcg acaacggcgt aatctgtgct tctgaacagt
     2041 ctgttgttgt tgttgactct gtttatgacg cagtacgtga acgtttcgca acccacggtg
     2101 gctatctgtt gcagggtaaa gagctgaaag ctgttcagga cgttatcctg aaaaacggtg
     2161 cgctgaacgc ggctatcgtt ggtcagccag cctataaaat tgctgaactg gcaggcttct
     2221 ctgtaccaga aaacaccaag attctgatcg gtgaagtaac cgttgttgat gaaagcgaac
     2281 cgttcgcaca tgaaaaactg tccccgactt tggcaatgta ccgtgctaag gatttcgaag
     2341 acgcggtaga aaaagcagaa aaactggttg ctatgggcgg tatcggtcat acctcttgcc
     2401 tgtacactga ccaggataac caaccggctc gcgtttctta cttcggtcag aaaatgaaaa
     2461 cggctcgtat cctgattaac accccggctt ctcagggtgg tatcggtgac ctgtataact
     2521 tcaaactcgc accttccctg actctgggtt gtggttcctg gggtggtaac tccatctctg
     2581 aaaacgttgg tccgaaacac ctgatcaaca agaaaaccgt tgctaagcga gctgaaaaca
     2641 tgttgtggca caaacttccg aaatctatct acttccgccg tggctccctg ccaatcgcgc
     2701 tggatgaagt gattactgat ggccacaaac gtgcgctcat cgtgactgac cgcttcctgt
     2761 tcaacaatgg ctatgctgat cagatcactt ccgtattgaa agcagcaggc gttgaaactg
     2821 aagtcttctt cgaagtagaa gctgacccga ccctgagcat cgttcgtaaa ggtgcagaac
     2881 tggcaaactc cttcaaacca gacgtgatta tcgcgctggg tggtggttcc ccgatggacg
     2941 ctgcgaagat catgtgggtt atgtacgaac atccggaaac tcacttcgaa gaactggcgc
     3001 tgcgctttat ggatatccgt aaacgtatct acaagttccc gaaaatgggt gtgaaagcga
     3061 aaatgatcgc tgtcaccacc acttctggta caggttctga agtcactccg tttgcggttg
     3121 taactgacga cactactggt cagaaatatc cgctggcaga ctatgcactg accccggata
     3181 tggcgattgt cgacgccaac cttgttatgg acatgccgaa gtccctgtgt gctttcggtg
     3241 gtctggacgc agtaactcac gccatggaag cttatgtttc tgtactggca tctgagttct
     3301 ctgatggtca ggctctgcag gcactgaaac tactgaaaga atatctgcca gcgtcctacc
     3361 acgaagggtc taaaaatccg gtagcgcgtg aacgtgttca cagtgcagcg actatcgcgg
     3421 gtatcgcgtt tgcgaacgcc ttcctgggtg tatgtcactc aatggcgcac aaactgggtt
     3481 cccagttcca tattccgcac ggtctggcaa acgccctgct gatttgtaac gttattcgct
     3541 acaacgcgaa tgacaacccg accaagcaga ctgcattcag ccagtatgac cgtccgcagg
     3601 ctcgccgtcg ttatgctgaa attgctgacc acctgggtct gagcgcaccg ggcgaccgta
     3661 ctgctgctaa gatcgagaaa ctgctggcat ggctggaaac actgaaagct gaactgggta
     3721 ttccgaaatc tatccgtgaa gctggcgttc aggaagcaga cttcctggcg aacgtggata
     3781 aactgtctga agatgcattc gatgaccagt gcaccggcgc taacccgcgt tacccgctga
     3841 tctccgagct gaaacagatt ctgctggata cttactacgg tcgtgattat gtagaaggcg
     3901 aaactgcagc gaagaaagaa gccgctccgg ctaaagctga gaaaaaagcg aaaaaatccg
     3961 cttaatcagt agcgctgtct ggcaacataa cggccccttc tggggtaatg ccagtcagtt
     4021 aaggatcagt tgaccgatcc agtggctgtg taagaatccg gaaacgctca ctcgtttccg
     4081 gattttttta tgctcattgg acaggccctt gatttggtat cccgttacga ttctctgcgt
     4141 aacccactga cttctctggg ggattacctc gaccccgaac tcatctctcg ttgccttgcc
     4201 gaatcaggta ctgtaacgct acgcaagcgc cgtcttcccc tcgaaatgat ggtctggtgt
     4261 attgttggca tggcgcttga gcgtaaagaa cctcttcacc agattgtgaa tcgcctggat
     4321 atcatgctgc cgggcaatcg ccccttcgtt gcccccagtg ccgttattca gacccgccag
     4381 cgtctgggaa ttgaggctgt ccgccgcgtg ttcacgaaaa cagcgcagct ctggcataac
     4441 accacgccgc atccgcactg gtgcggcctg accctgctgg ccatcgatgg tgtgttctgg
     4501 cgcacaccgg atacaccaga gaacgatgca gccttccccc gccagacaca tgccgggaac
     4561 ccggcgctct acctgcaggt caaaatggtc tgccagatgg aactgaccag ccatctgctg
     4621 acggctgcag ccttcggcac gatgaagaac agcgaaaatg agcttgctga gcaacttata
     4681 gaacaaaccg gcgataacac cctgacgtta atggataaag gttattactc actgggactg
     4741 ttaaatacct ggagcctggc gggagaacac cgccactgga tgatccctct cagaaaggga
     4801 gcgcaatatg aagagatcag aaaactgggt aaaggcgatc atctggtgaa gctgaacacc
     4861 agcccgcagg cacgaaaaaa gtggccggga ctgggaaatg aggtgacagt ccgcctgctg
     4921 accgtgacgc gcaaaggaaa agtctgccat ctgctgacgt cgatgacgga cgccatgcgc
     4981 ttccccggga cgtataccgg agctgatgcg cgatcttgca agtatgggac aactagtgaa
     5041 attaccgaca agaagagaaa gggccttccc gagagtgata aaggagaggc cctggaaata
     5101 ctccacagcc ccgaaaaaga gccagtcagt tgcttaactg actggcatta ccccttctgg
     5161 ggccgttttt tattttccca aagcaacttt tccataaacc ggcagcattg tccttcatca
     5221 tatttgcgac gatgtataac gcctacacac agcgatattg tactttacag gttacaagtc
     5281 aatgtcggtg tataagcgct ctgtgaggcg tataacggcg ttaaaaaact gccgagaagg
     5341 gtgtatagcc cggaagaagt gcgtaaaacg aactgacagg gataaaagcg ccctgctcac
     5401 cctgtcagta aagaaattct tattaatcgt ggcgatgcct ttcctgaata gccgttaatg
     5461 agccgacttc taacgcctct ttatagtgtt tacggcatac agaaacgtag cgttcattac
     5521 caccaataac cacctgctca ccttcgttat aaggtctgcc tgcttgatca agacgcagca
     5581 ccatgcttgc tttacggcca caaaaacaga tggtttttaa ttcaaccagt ttgtcggacc
     5641 atgccagtaa gtattgactg ccaataaata attcacctcg aaaatcggta cgtagaccat
     5701 aacaaagtac gggtatatcg agttgatcga caacctccga taattcatat gcttgttgtc
     5761 tggttaaaaa ctggcattca tcaaccagta cgcaatgaat tgcctgctgt tcatgttccg
     5821 cacgaatctc atcaaataat gatgaatttt ggttaaataa ttttgcaggt gatgacaaac
     5881 ctatacgcga actgactttc ccggcaccaa agcgatcatc aatttctgcc gtatatacga
     5941 cagtgcgcat gccgcgttcc tggtaattgt atgaagattg caacaatgct gtagacttac
     6001 ccgcattcat tgcggaatag tagaaatata gctgtgccat cagccacagg ccctcaatga
     6061 tatgaaataa agttggctgg agtttatcat aattcgtcag tttttcagct caacagtatg
     6121 cacagaagtg gtatatgcgt tctcccttac gaagccttgc ataatccttc tgagctatca
     6181 ttacaactgc ctcgcttgtt ataagcgggt aaatgactgc cggtaactat tcacaatctt
     6241 taacctgttg cgcatgtaat agccctctgt tgacctccag gagatagtgc aatactaagt
     6301 ccctgctctt attgcgactg ttctactttt catcattcgc ttaataggga attctcgtaa
     6361 acacgactaa tacagaagac tgaaaggtcg tcagcctacg attatctccc cataaaatgt
     6421 gacatgaatc aggaagtttt aacctcacgt gctgcgaaat catcggtgca aatagggcta
     6481 tatgccgcgt cttttctggc taattttatg aaaagatatt tattggcggc acaaaataaa
     6541 gaacaatttt gaattcctta cattcctggc tattgcacaa ctgaatttaa ggctctatta
     6601 ttacctcaac aaaccacccc aatataagtt tgagattact acaatgagcg aagcacttaa
     6661 aattctgaac aacatccgta ctcttcgtgc gcaggcaaga gaatgtacac ttgaaacgct
     6721 ggaagaaatg ctggaaaaat tagaagttgt tgttaacgaa cgtcgcgaag aagaaagcgc
     6781 ggctgctgct gaagttgaag agcgcactcg taaactgcag caatatcgcg aaatgctgat
     6841 cgctgacggt attgacccga acgaactgct gaatagcctt gctgccgtta aatctggcac
     6901 caaagctaag cgtgctcagc gtccggcaaa atatagctac gttgacgaaa acggcgaaac
     6961 taaaacctgg actggccagg gccgtactcc agctgtaatc aaaaaagcaa tggatgagca
     7021 aggtaaatcc ctcgacgatt tcctgatcaa gcaataatct tttgtagatt gcacatgcta
     7081 aaaatcccgc catcggcggg attttttatt gtccggttta agacaattta ataagaataa
     7141 gctataaaaa aaacggcgtc gattgctcaa caccgtttcg tggaaaacac cgatacggat
     7201 gttacttctt aatgcccatc tcttcttcaa gccaggcttt aaattccgtg ccaagggtgt
     7261 tatgacgaat accgtattca acgaaggcct gcatgtaacc taatttatta ccgcagtcat
     7321 ggctcttccc tttcatatga taggcttcaa ccgtttcttt ttcgatcagc atatcaattg
     7381 cgtcggtgag ctgaatttca tcaccagctc caggaggggt ttttgccagc aacggccaaa
     7441 tatccgcgct aagtacgtaa cgacccacaa tagcgagatt agacggcgca acatccgctt
     7501 ttggtttttc aaccacgcca accatcggta cgctttcacc cggcgctaat tcaacgcctt
     7561 tgcaatccac aacgccatat gcggtcacat cagcaaccgg ttcaaccatg atctggctat
     7621 gacccgtttc atcaaagcgg cggatcatct ctgccaggtt atcctgtgat aaatcggatt
     7681 catattcatc cagaataacg tcaggcaaaa taacagccac aggttcatca cccactaccg
     7741 ggtgagcaca caataccgcg tgtcccaggc ctttcgccag cccctgacga acttgcataa
     7801 tagtcacgtg cggcggacaa atagactgca cttcatcaag cagttgacgt tttacacgtt
     7861 tttccagcat tgcttccagt tcaaaactgg tatcaaagtg gttttcaata gagtttttag
     7921 atgagtgagt aaccagcaca atttcagtaa tgccagccgc aatacattca ttcacgacgt
     7981 attgaattaa tggcttatcg acaagtggca gcatctcttt cgggatggct ttcgtcgccg
     8041 gcaacatcct ggttcctaat cccgctacgg ggataacggc ttttttgact ttcgtattaa
     8101 tggcagccat ttaaattctc ctggactgtt cgtgttttga acgtgttcat atttctgtat
     8161 cgcatcccag tatatcagca ccccgaaggg gctaagttct gaaaaggagg cgagttagca
     8221 taattaagac aattacttat acgaatagcg acgatagtac caccagccga cattagcagg
     8281 taatgcaaat ttagcccgtg ttatcgtttg ctcattctgc agacaacatc aagcgcagcc
     8341 gaccaccggt tccccatatt tggcattgcc aggcatcgca tcgctggctc aattgattca
     8401 aataagcgtt acctaaagtg cctaacggaa caccattact gatttgcacc tgatgttcgc
     8461 cggtatttaa cgtcgcattc agacccgcag aaaccagaat gagatttttc agttcgcgat
     8521 gataatagcc aactaatagc ggaaactgcc ccggcagatt ggcctgacgg agtaaatggt
     8581 ttacctgctt caataacgcg cccaactctg gcaaccgttg attttggtgt gcaagctgtt
     8641 cctgtaataa tccgttaaac aatgcgcgta ataacaaggc agcaagtacg ccattatgtc
     8701 cagctcgggt gacatcaagg caataaaatg ccagatcgtt ttccgaaagt gcggcaatat
     8761 caagcaccag gccgggtttg tccgcggcaa ctaattgacg ataattaacc cggcattggg
     8821 aaatcacctg ctgaaccggc ggttgtagtt cctgtaataa tttcgccgct gcagcagggt
     8881 tatcaaccat tgcatcccag tcgcgaaaaa gcctttcctc ttcctcaacg cgggaattaa
     8941 acatgctggg atagagacag gcaaaaacca tctcgcgcaa gcgattcaga tctttaaccg
     9001 gtttcagcaa aacatcttca acgcccagac gtaacgcttt ggcaatatct gccatatttt
     9061 cagtggcaga tatcaccaga actggtgtct ggtcacctct gttgcgtata tgttccagca
     9121 gtttaagccc gttcattcgt ggcatcgcga tatcacatat catcaggtct ggagtgaaac
     9181 ctcccagcaa ctcaagggca tccaccccat cagccgccag taccgttgtc gctcccaatg
     9241 aggaaaacca cgaatccaga agcgagcgaa atacctgctc atcttcaaca atgagaatct
     9301 gttttccgac caatggctgc gtcatgttct ctcccctgac tggctttact caatagtggc
     9361 atgctattgc cacctacgcc tgtcagaatt aacttaagtg tagataaaaa ttctggtcaa
     9421 atgttggtgc gtaccaacgg caaaagttcg tccattttct tttccactgc cagctgtccg
     9481 gccgcaatgg cagcgtgcgc gcgatggaaa tcaagcgtag atatttgcgg gcaaacaggt
     9541 tgaatcagaa tatcgggcgg atcacctgcc atgcggttcc ttttaaggcg gttctccagc
     9601 acctggatag aagtggtcat aatctctgtt gccgttggcg ctgtcaccgc ccgacgtgtc
     9661 gttatgctgc ctaacctttc tttcagacgc gcatgccacg gcagagaatc accattctcg
     9721 ctattttctt cactgacatt aaaggagagc aaatcttgtt gcatcaaatg agcatcgtgc
     9781 tgcaggtcga ccgctatcac aatatcagcc cccaatgcac gcgtgaggga aataggaatt
     9841 gggttaacta cagcgccatc aaccagccag tagccgttat gcgcaacggg tgccattagt
     9901 cctggaatac tgcatgatgc acgaatagca agatggagat cgccttcagt aaaccataat
     9961 tcacgcccag tacttaaatt ggtggcaaca gccgcgaagc gacgggaaca attttcgatc
    10021 tctgtttccg gcattatttc gcgatattga ttgaagacac gctcgccgcg cagtaaccca
    10081 ccgcgctgcc aggagagatc catcaggcgt aaaacatccc aataactgaa agaggtcacc
    10141 caatcttcca gcgcagataa tcgatcgcat gcataggcag cgcccaccag cgaaccaatt
    10201 gaacatcctg caacgatatc aatttcaata cccacttttt ttagcgcatt aattacgcca
    10261 atatgcgacc aacctcttgc cgcgccagat cccagcgcca gccctatttt tatctttctc
    10321 attattcccg caagattccc ctggaatgca atcgtagcca catcgctact gctcggttaa
    10381 catagtgcta ctcaggcggt taatacgccg ttattgtttc cagggagatt atttgtgtct
    10441 cagctttgtc cctgtggtag tgctgtcgag tatagcctat gttgccaccc ttatgtgtct
    10501 ggtgaaaagg ttgcacctga tccagaacat ctcatgcgtt cgcgttactg cgcttttgtg
    10561 atgcaagacg cagattattt aataaagacc tggcatccat cttgtggagc agcggcgtta
    10621 cgtgccgaat tgattgccgg atttgcgcat actgaatggt tgggattgac cgtatttgaa
    10681 cattgctggc aggacggcgg cgatatcgga tttgtcagtt ttgttgctcg atttaccgag
    10741 ggtggcaaaa caggtgcaat aattgaacgt tcgcgatttt tgaaagaaaa cggtcaatgg
    10801 tactatattg acggtacacg tccgcagttt ggccgcaacg atccctgccc ttgtggttca
    10861 ggtaaaaaat ttaaaaagtg ctgcggccaa taatggttga cggtacggtt tagcaaacac
    10921 tctcaacaag gtttttccag caatgcattc actccaacgt aaagttctgc gtactatttg
    10981 tccggaccaa aaaggtctga tcgcacgtat taccaatatt tgctacaagc acgagttaaa
    11041 tatcgtgcag aacaatgaat ttgtagatca tcgtaccggg cgctttttta tgcgcacgga
    11101 actggaaggg atttttaatg attccaccct gctggcagat ctcgatagcg cattgccaga
    11161 aggctccgtg cgtgagctga atcctgccgg tcgtcgccgg atagtgattc tggtcactaa
    11221 agaagcacat tgccttggcg atttgctgat gaaagccaat tacggcggcc tggatgtcga
    11281 aatcgcggca gttattggta accacgataa tttacgttct ctggttgagc gttttgatat
    11341 tccgtttgag ctggtaagcc atgaagggtt aagccgcaac gagcacgatc aaaagatggc
    11401 ggatgccatt gatgcttatc aacctgacta tgtggtgctg gcgaagtata tgcgggtatt
    11461 aacgccggaa tttgtggctc gcttcccgaa taagatcatc aatattcacc attccttcct
    11521 gccagcgttt atcggcgcac gtccttatca ccaggcctat gagcgtggcg tgaagattat
    11581 tggcgcaacc gctcactatg tgaatgacaa tctggacgaa ggtccaatca tcatgcagga
    11641 cgttattcat gtcgatcata cctacacagc tgaagatatg atgcgcgcag gtcgtgacgt
    11701 cgagaaaaac gtcttaagtc gtgcgctata caaagtactg gcgcagcgcg tctttgttta
    11761 cggtaatcgg acgattattc tttaatcgtc agccaaaata actggttagc tttaatccgt
    11821 tacggatgaa aatcacgcaa ccagttcatt tttctcaacg taacacttta cagcggcgcg
    11881 tcatttgata tgatgcgccc cgcttcccga taagggagca ggccagtaaa aagcattacc
    11941 ccgtggtggg gttcccgagc ggccaaaggg agcagactgt aaatctgccg tcatcgactt
    12001 cgaaggttcg aatccttccc ccaccaccat cactcgattc cattatcaaa ccttcggctt
    12061 ctcccccatt cgccgtaaaa actgctgctg tagcctcaac aaatccgcta acgtcaacaa
    12121 ctgataatcc agaccttgct ggctggcaat ttgcgcaata atgcggctta gcgccgaata
    12181 atggcgatga tgccagtgag gaaacagatg atgggtaagg tgcagattaa tcccacccag
    12241 ccagtagcca agccatgcag gttgcggcgt ccaatcaaag gtggtagcaa aggtatgcgc
    12301 cagtctgccc acgggcattt tgccctcttc tggtggtaac tgcgtatgtc ccttcgccca
    12361 gtgcgttcca ataatcaacg tcacaaaaat caatgacgcc agcagctggc tgagcagata
    12421 agtcagcagg atggtcataa aactaaaatc agacaaaagc cagggaatta tcagacacag
    12481 cgcacaatgc gccagtttcc cggctaagaa atatccccag ccagcaaagc ctcgcagggc
    12541 cagatgtcgc gtgaccggcg taaaccctgc gcgatcgagc cagtccacta cccagatata
    12601 ccagggaaag gtgagcgcgg cgaccagtgg ccaatagcga tgttgctggc gcataaaagg
    12661 tcgccaccgt tgccacggcg tctggcgaag aatgccattt tctgctgtat ctggatcgta
    12721 aaattcaata ttggtaaaac cgtgatgaaa gcgaacgtgg cgcacccgcc agcagtccgg
    12781 atctagcccc ataggaaaag caacccaccg gttaagccgg gcattggtct ttttaccacg
    12841 caaaaacgca tcatgcgaag cgtcatgcac cacattgacc gctaacaaca tggcgcaaaa
    12901 catcatcgcg agataccaca acagatacag tccccattga cgttgaaata acgccagtaa
    12961 gtaacaggag aaacaagcaa taatcaacac cgccattttg acgaatgccc atccgtcggc
    13021 ataccgatga tcgcctgatt tcgccagata aactttcgta ccgctttgca gttgtttcac
    13081 cagttgtgga tcatcggcag gaaaagtgat taacctgtcg ggattaaaca ggttcatact
    13141 gtttccttag cgatttacgt cctacgccga ttacaaataa caggcaatgg gccagcagaa
    13201 tacccactaa tcccttccag agcagagtac tccatgaaca catatagaca ataaaataga
    13261 gcagtgacaa cgtcacagag caccacaaaa tcatacccca atgctgcccg tcgctcatgc
    13321 cccccagcgc aacggagcct gccgccagca gaacaaataa tacggcttgc tcgctggtaa
    13381 taaactcaat gccatagcca tattgttgaa tgtagccaag caccagcata aacagcagga
    13441 tcacccctgc ccctaccgcc catggagaac tctggtagcg accttcaatc gctttgccaa
    13501 atgagcaaaa gcccattaag cgtaaaaatg gcagattact ctcgcgcatc gggttacagg
    13561 acaagcgact attttttacg ccgtagccta cgtcgtattc cggcccggca ctatacgaac
    13621 cgaaaagttt atcccacaac aaaaaggtgc caccgtaatt ggtatcggca tattgctttt
    13681 gattcagatg atggacgcga tgatgcgaag gcgtgatcag cactttttcc agccagccca
    13741 gtttcggcgt tagtgcgtta tggttaaaaa attgccaact atagtgaata atagaaaccg
    13801 ccaaaaagac ctgtaacggc acaccaagca tcgccagcag cataaaaaac gggattgagg
    13861 ttaacgagga gtaccacgag ttccgcacgc ctaatgacag gttgtagtgt tccccctgat
    13921 gatgcaccac atgtaccgcc cacagcgggc gcatttcgtg atgccagcga tgtagccagt
    13981 aaaagccgaa atcccacgcc aggatagtaa acaaccacag caggatcacc ggcacattat
    14041 caaacagtcc aaggctgaag tgattgacca ccaggccata gcagagcact tccaggcagc
    14101 gaaataacca cagcatcata tgaccggagt tgatattaaa aataacgtcc agccagtcca
    14161 ctttttgccg cccggtgaat tgcaacacgc ccgcttcggc taacaccaac agcaccatcg
    14221 atatgatggg gaataataaa tcactcatat tgtgtcttcc ttttctcgca tgttgcgcat
    14281 atccaaccgc aaatcgacgc cagcaacgcg ccgcctacta aatcgataaa taaatgtctg
    14341 cgtaattgca gaatcgaaaa ggcgattgct atcgcccaga ccacgctcat tagcgtcagc
    14401 caacggttcc cctttttagc aatcgcccat accgccaaca gcgttagcgc ggcgtgtaag
    14461 gagggaaaac agttctggct tgagtcaatg gcgatcagcc tgttgagtgc gcccgcggat
    14521 atccccatct gatcaaacga gggataatgc atggttgtgg gccagagaag ataaaatacg
    14581 ccagccacca gtgcggaaat ttgcattgaa cgggccagcc agcgcaggtg gcaaagtggc
    14641 gtggataaat aacctaacgg cacaatgaaa aaaaacgaga gatacagcca cacagcggag
    14701 ggggaaaacg caatcaaatt atcaattatt gacggtgtta attgatatcc ctcaccttgc
    14761 aggtagtcgc taaagttgta gataacccca acagttcccc agcccagcag catctgtttc
    14821 aggcgtagca gtgaattgct cattcccgcc cccactgacg cacaatgcgc cgccgcttgg
    14881 ctgtaaaaga gactggcaca gtttgcagtt ccagcgacca aattaattgc ccgacagcga
    14941 tatcctggcg tataaacaag gcattcaacg cctcccggca atgctccagc atccacagct
    15001 cgcaatcggc gattaacgtc aattgtgctg acccggtttg tagcaggcga taatcgactg
    15061 ttagcggcag tgtcattgcc agcacacgac tacaggcatc ggcaaaaacg gtctgaatat
    15121 cgccggatct tgttggtagt tgcagttggt catcctgccg cccttcgata cgcgcaatcg
    15181 ccattgttgc actgccacag gggcatggct gttcgcttgc caccagcaca tcatccagtc
    15241 gataacgcac tatcggctgt gtggtgcggg taaagtctgt gacgatcggc acgaaacggt
    15301 gctcatccag ccattgcggt tcaatgtgaa caaactcttc gttgagatgt aacgttccac
    15361 accggcaggt ggaagcgaga aagccttctg tcgcctgata aatttccccc acattgtgga
    15421 acacgttgcg cagtaattcc cgatcctgcg gctccagaac ttccgccacc gaaatcactt
    15481 ttttgacctt tagcgtcaat tcgccggcca tcacctccag cgccagtgcg cgtaatacct
    15541 gagctggcgc gacaataatc gtgggcgctc gctgttggag aagtttgagt tgcgcctgaa
    15601 atggcgctag cagatcgtaa aaatcgagac ttaaccagcg attattcacg ctttgataca
    15661 ggttgttgtc cgcgcgcaga aacagcgcca cccgctcttt ggcaaataaa tcatcgggca
    15721 acacttttgc cagcacaccg gcggcccaca tctgttgctc gtgcgggcta acaacaaaaa
    15781 gtccacgacg cccggatgta cctgatgaaa gcccaacgct gaacttacca acgcagggtt
    15841 tgaaatcacg actctgctca ctgcgcattg cacaatcgag aagttcgtcg cgctttaaac
    15901 ctgcggtatt catctcatcg aagtgcgtca tcattaacgc tttatccatc attggccact
    15961 gattaaaggg caacgtcaga taacgctgaa accacgggct atgagataag acattttgcc
    16021 ggaactgatg cagccttttt gcctgccagt tttccagtgc ctcgcgatcg gtgaaatgca
    16081 gcctgcgggt acggaagtag cgccagatca acatcaacgg gatcataaat ccccctcatg
    16141 gcacaaacga atctccacca caccgttttg ccaaagctga tggagttgcc caagcgtgtg
    16201 gtagtaggcg tggctgtcgg acataaccag atgcgccagt ggtgaaggcc ctcgcagctc
    16261 ccggtagtta gctggcgccc atgccgcatc gccagccagt aaagtccagc cgttgtcggt
    16321 taacacaaac gcgccaatat gtccggcagc atgtcctgga agcggcacga gaataatttc
    16381 gccattgctt tctggcagca aatagccgca ctcaaacggt gccagttgcg cgggcaaatt
    16441 caccgatgcg aaactttcaa taaagcgcag tgaggagtca aaattctccg ggattagccc
    16501 tggcacaaag gcctggcgca gcgcagtgac accacgcaga gttcttactt gccgccagcc
    16561 ttcaccagaa cagatgcagg aaacatcagg gaaatcacgc aggccagcaa tatgatcagc
    16621 gtgaaaatgc gacagaataa tcgcatcaac atctttcgct gcgaggccgg tggcagaaag
    16681 ttgtcgcacc agcgcctgtt gcggatcgaa ataaaccggc gtgacctggc gataaagacg
    16741 aaacacacca gattgcgtgt agtgttcgaa ccacgatgaa tagcctgtgt cccacagcca
    16801 ccgtttgtcg cccacctcca gcagccaggc gcgggcgggg aatttgcaca ctttcagccc
    16861 tgcccctttc agcgcaatac agctaatatg ggtacaccag cccgtttcaa atgcggttat
    16921 ttttgccatg ttccctcagc cattgtcccg tgattgcaat accttgttcc atcgaaaagc
    16981 gtgggcgata tcccagctct gtaatggcct tttgcgcact cagcgtcata tcgaaacaga
    17041 gtgttccggc gctgtatcgt gtgataacag gttcctgatt aacgcacttc ccatacagtt
    17101 ccaggccacg cgcgatcaga gaaagcagcg accagggaac agcagcaatg cggtaactca
    17161 agcccagttg ctggtgcagc aaggcgtcaa gcatcaccga tagctgctgc ggctggtgat
    17221 tgctgatgtt gtaaatactg cccgagcgta agccttcaac gttcgttgcc agccgcatgg
    17281 catacaccac gttttgcacg aacgtgagat ccaataacgc gtcgcccccg cgcggcaaac
    17341 gtaagcaacc gccgccctgt tgcaattgct gcatcagacg aggaacaatt acctggtcat
    17401 gggggccaaa caaaccacgg ggacgcaaaa gaataaatct cgtggcagaa tatcgagaaa
    17461 cagcgccagc aataacctgc tcagcggcat atttactgct ggcataatgg ctggagaatg
    17521 ctcgcgcaag ataagtttct ggcaggtcat agtgatgttg aaaatcaaag taaaccgcag
    17581 gcgtggagat atggataaac cggggaatat tcagcctgcc tgccgcctgt gccagagtgt
    17641 gggtcacttt gacattggca agccagaacg ccgttttgtc gccccagggc gaggactttg
    17701 ccgcacagtg ccagacggca tcgcagccgt ccatcaggcg ggcgcagtca tcctcggttg
    17761 cctcggttaa atcgagagcc acaaactgtg cgcctgcgtt tgtcaggctt ctgccaacct
    17821 cacaatttcg ccctgttgcc acaaccgact cacccgattg caacaaatat tgcacggcat
    17881 ttcgcccaag gccgctggtc gcccctgtta cgaggatctt cacggtaaaa gcaccatgcc
    17941 agccagcgtt agtcccgctg ccgtgccaat caacattaca ggtttatccg ctgtgaaacg
    18001 accgctaatc actgcttcat gcagcgccgt ggggatagag gccgcgacct gattgccgta
    18061 aaaacgatac acatcgatga gcgcagattc cggcactcgc agccgtttac gcatatgttc
    18121 aagggaaaga tgactggcct gatgcggaac gaccgtgccg atatcagaca gttgcaggct
    18181 gcttttttgc agcaaccgag aaaaataatc ttcaataagc gaagaagcaa aacgaaacag
    18241 cggtttcccc tgcatatgaa aaagaaagtc gctatcactc acaccagagc gaggattacg
    18301 ccgcgtaccg ccagcacgaa tttcacacag ttcgctgcct tcgggataca tttcgatcag
    18361 gctagcgata atcccgctgc gcccatcgcc ccgctcaaca atcgcgcacg ccgcgccatc
    18421 accaaaaatt aatgaagact cttcatgatc ccagtcgata ccgcgtgaag caatatctgc
    18481 cgaaacgatg gcaatgcgtc gatatgctcc acagtttaat aatccggcag cgacttcaag
    18541 agcggaaata aagctcacac agctactgtt gatatcaaac ccggcaacgc ctacgggcat
    18601 ggctgcggct ttaagaatat gaattgcgct acagggtaat gcctgtacgg caatggccga
    18661 tgcacaaatt agcagatcaa tatcagaagg atttaaggct gccgctacta acgcattatg
    18721 taaagcctct gccgccagtt cggcctggct ggcgtcgttg cgggcaaaat agcgccagca
    18781 aataccggaa cgtcgctcaa catagccaac tggcttattt aactgaacat ccagttgttt
    18841 agaagtgatt tgatcagggg gtaaggcgat ccccgtggcg attattttta acgccgcgga
    18901 gatcgtcggt tgattaggtc gagccacggt cagtgctcct tcactgctcc ataggtggac
    18961 caatcatata gatgaagtac gtttccctac aatccgaaaa atcggcagtg tgtaaccgcc
    19021 gatgtttggt ttagataatg tttggtttag ataattagtg acgagcgcgc accagctgat
    19081 acttacgcgt cagatactcc accgggacac tccagatgtg taccagacgc gagaacggga
    19141 acagcaggaa taacgtcatc cccaacacca ggtgcagacg gaagataaac gccacgccat
    19201 caagatgttg agaagcgcca ccgtggaagg tcactaccga ctgcgcccag ccaaccagtt
    19261 tcatcatctc gctaccgtcc atatgctgag cagagaacgg aatggtcagc aggcccagcg
    19321 cgcactggat aacgagcagc gacaggatca ggatatccgc tccggtagtc gttgcacgca
    19381 cgcgtgggct gaacagacgg cgtttcaaca acagcacgcc gccaatcaga cacagtacgc
    19441 cgctggcacc accagcaaac atcgccattt tctgcttcac ttcaatcggc agccaggctt
    19501 catacatcca gtgcggcgtc agcataccga agaagtgacc gacaaaaatc cccagaatac
    19561 cgatatggaa caggttcgac gccagattca tccctttgcg atccagcatc tggctggacg
    19621 ccgcgcgcca ggtatactgc ccgtagtcat aacgcagcca gctaccaatc aggaagaccg
    19681 ccccggctat gtacgggtag atatcaaaga agaacatatt caggaattgc attagtgctg
    19741 tcctccggtg gtgatattca gatattgcgg cgcgacggca ccggcaaagc gacgctgatg
    19801 agcagtgatt gctgaatcac cgcagccttt gtcagcaaag aatttaacct gctcttcttc
    19861 ccagactgca tccagcgcct gcggcgtatc gtcgcgcgct tcgtcggcaa ttttttccgc
    19921 cactttgtcg ctgtcgattg cggtattcgc cagtttcagc agcagatcaa acagcacggc
    19981 ataacggctt tcacgctgtt gcagacgcgc actcagcaat gccagaatcg gcgcaatatc
    20041 tttcaaaccc tccacggctt cgctttgcgg cagctgcgcc aggtactcca gatacagcgg
    20101 cagatggtcg ggcaattcgc ggctgtttaa ctgcaagccg tgctgctcgt actgcgccag
    20161 cagatccacc atcgcctggc cgcggtcacg ggattcaccg tgaacatgtt caaatagcaa
    20221 cagtgacgtg gcgcggccac ggtcgaacag ttcgctgtac tgcgcctggg catcgagcgg
    20281 atccatcgtc gttaaatcgc gcaggaaaat gcccagcgca tgggcatcct cttttggcag
    20341 atttttcgac gcggcaatcg cctcaaacat ctcttgttga tgctgccata aggcagcatc
    20401 cggatattca aggagacgcg atacaatcac gagttcgatc atgggtgcgg ctccgttttg
    20461 ctggttacgt cgatggcatc gatacggcgg ctgttgaaca gattgaattt ggtatctgaa
    20521 ccgtggcagc catcaccaaa ggtaaagccg cagccatttt tctccgggaa ggcttcccgc
    20581 gccagttcac gatgactgct cggcaccaca aagcgatctt cgtagttggc aatagccaga
    20641 taacgataca tctcctgtgc ctgggcttcg gtcagaccga cctcttccag cgcacgggta
    20701 tcgactttac cgtcaacggt ttcagcacgt ttgtaatgac gcatcgccag catacgtttc
    20761 agcgcgcgca gtaccggttt ggtatcaccg gcggtcagca gattcgccag atactgtacc
    20821 ggaatacgca ggctttcgac gtctggcaga atgccgttgc tgcccagttc acccgcgtct
    20881 gccgcagact gaatcggcga cagaggcggc acgtaccaga ccatcggcaa tgtgcgatat
    20941 tccggatgca gcggcagcgc cagtttccat tccatcgcca ttttataaac tggcgactgc
    21001 tgcgcagcct caataacgct cagcggaata ccgtctttaa tcgcctgctc gatgactttc
    21061 ggatcgttcg gatcgaggaa cacatccagc tgacgctggt agagatcttt ctcgttctcg
    21121 gtacttgccg cacgttcaat cgcgtcggca tcgtacagca gcacgccaag ataacggata
    21181 cgaccgacac aggtttctga acatattgtc ggctggccgg cttcaatacg cggatagcag
    21241 aagatgcact tctcagactt accgctcttc cagttgaagt agattttttt gtacgggcat
    21301 ccggtgatgc acatacgcca gccacggcat ttatcctggt cgatcaggac gatgccgtct
    21361 tcttcacgct tgtaaatcgc accgctcggg caggtcgcca cacatgccgg gttcagacag
    21421 tgttcgcaca ggcgcggcaa atacatcatg aaggtgtttt cgaactggct atacatcgcc
    21481 ttctggatgt tgtcgaagtt cttgtctttc gccagcttgt caaactcacc gcccaggtca
    21541 tcttcccagt tcggcccttt ttcgattttc gccatccgtt cgccggtaat cagcgaacgc
    21601 ggacgggcaa tcggctgtga tttgctgcct tccggcgcgg tatgcaggtt ctgataatca
    21661 aaatcaaacg gctcgtaata atcgtcgatc cccggcagat gcgggttagc gaagatttta
    21721 cccagcagca tggcacggtt acccatgcgc ggctgcagtt tgccgttgat tttacggatc
    21781 cagccgcctt tgtatttttc ctggttttcc cagtcagtcg ggaagccctg gcccggcttg
    21841 gtttccacgt tgttgaacca cgcgtattcc acgccttcac ggctggtcca gacgttttta
    21901 caggtcactg aacaggtgtg gcagccgatg cacttatcga gattcagcac catgccgact
    21961 tgtgaacgaa ttttcatttt acgctctcct gtacctggtc attgccttcg ccatctaacc
    22021 agtcaatgtt cttcatctta cgcactacaa caaactcatc gcggttagaa cctacggtgc
    22081 cgtagtagtt aaagccatat gccagatggg catagccgcc gatcatatgc gtcggtttcg
    22141 gcgtgatacg ggtgaccgag ttatggatac caccacgctg ttgggtaatt tccgaaccag
    22201 gcaggttaac gatacgttcc tgcgcgtggt acatcatggt catccctgcc ggaacacgct
    22261 ggctgacaac cgcacgggca gtcagtgcac cgttgctgtt gaagacttca atccagtcgt
    22321 tatcggcgat acccagctct ttggcatcgg cttcactcag ccagaccacc ggaccaccgc
    22381 gacctaaagt cagcatcaac aggttgtcgc tgtaggtgga gtggataccc cacttctggt
    22441 gtggcgtcag gaagttgagc gctttttccg ggttgccatt ggatttctgg cccatcactt
    22501 ctttcaccga acgggtgtcg atcggcggac gataaaccag caggctttca ccgaaatcac
    22561 gcatccattg gtgatcctga tacagctgct gacggccaga gagcgtacgc cacgggatca
    22621 gctcgtgaac gttggtgtaa ccggcgttgt aagaaacgtg ttcatcttca aggccagacc
    22681 atgtcgggct ggagatgatt ttgcgcggct gtgcctgaat atcgcggaag cgaatcttct
    22741 cgtcttcttt attcagcgcc agatgcgtat ggtcacggcc ggtaaactcg ctcagggcag
    22801 cccaggcttt tacagctacc tgaccgttgg tttccggtgc cagcgtcagg atcatctctg
    22861 ccgcatcaat tgcggtattg agcatcggct gaccttttgc cggaccttcc gcttttgtgt
    22921 agttgagctt acgcagcaga tccatctcgc tctgggtgtt ccaggcaatc cctttaccgc
    22981 cgttaccgat tttctccatc agcgggccga tagaggtaaa gcgttcgtaa gttgccggat
    23041 aatcgcgctc tacgaccata atgtgcggtg cggttttacc tgggatcagg tcgcattcgc
    23101 cttttttcca gtctttcaca tccagcggtt gcgccagttc agcggcagag tcgtgctgga
    23161 taggcagcgt gacgatgtca gtttctttac ccagatgacc aacgcacact tcggagaatt
    23221 tcttcgcgat ggctttgtag atttcccagt cgcttttcgc ttcccaggcc ggatcgaccg
    23281 ccgcagacag cgggtgaata aacggatgca tatccgaagt attcatgtcg tctttttcgt
    23341 accaggtcgc cgtcggcaaa atgatgtcgg aatagagaca ggtgctcgac agacggaagt
    23401 ccagcgtcac caccagatcc agcttgcctt ccagaccgtt gtcctgccag tccacttctt
    23461 ccggcttcac gccgccctgc tgccccaggt ctttgccctg gataccgtgc tccgtcccca
    23521 gcaggtactt gagcataaac tcatgacctt taccggaaga accgagcagg ttagaacgcc
    23581 agatgaacag gttacgcggg tggtttttac cgttttccgg ctgttccgca gcgaaacgga
    23641 tagaaccctc tttcagggat ttcaccgtat agtccaccgg attcatcccg gctttttctg
    23701 cttcgcgagc gatagtcagc gggttagtgc ctaactgcgg cgcagacggc agccagccca
    23761 tgcgttctgc acggacgtta aagtcgatca agtgtccggt atagcgagat ttatccgcca
    23821 tcggcgacag cagttcttcc gcagtcacgg tttcataacg ccactggctg gagtggttat
    23881 agaaataaga ggtactgttc atgtgacgcg ccggacgctg ccagtcgagg gcaaacgcca
    23941 gcggctgcca gccggtttgc ggacgcagtt tttcctgacc tacatagtgc gcccagccgc
    24001 ccccgctctg accgacacaa ccgcagaaaa tcagcatgtt gatcagacca cgatagttca
    24061 tgtccaggtg ataccagtgg ttcagccctg cgccgacgat aatcatcgaa cgaccgtgcg
    24121 ttttatcagc gttatcggca aattcacggg cgatgcgaat aatctggctg cgagaaacgc
    24181 cggtaatctg ctctgcccag gcaggggtgt aagctttcac atcgtcatag ctggttgcac
    24241 agttaacgtc gttcaggcca cgttccagac cgtagtttgc cagcgtcaga tcataaacgg
    24301 tggtcaccag ggcggtgctg ccatcagcca gttgcaggcg tttcaccggc agcttatgca
    24361 gcagcacgtt ttccagttcc actttgttga agtgttccgt gccatcgcca ccaaagtacg
    24421 ggaagcccac ctcagcgatc tcatcctggc tacccagcag gctcagttgc agctcggttt
    24481 cttcgccagt tttgccgtcg cgctgctcaa gattccattt gcccttctcg ccccagcgga
    24541 agccaataga accgttcggc gcaaccattt caccattggt attaaaggcg acagttttcc
    24601 attccggatt gttttcctgg cccagtgcat ccaccagatc agcagcgcgc agcatacgac
    24661 ccgcagcgta gtaaccgtcg cgctcttcca gcatcaccag catcggcatg tcggtgtagc
    24721 ggcgcacata gtcggtgaaa tactggcttg ggttgtcgag gtggaattca cgcagcatta
    24781 cgtgacccat tgccagcgcc attgccgcat cggtgccctg tttcggtgcc agccacagat
    24841 cgcacagttt ggcgatttca gcgtagtctg gtgtgacggc aacagttttg gtccctttgt
    24901 aacgcacttc agtaaagaag tgagcatccg gggtacgcgt ctgcggcacg tttgaacccc
    24961 aggcgatgat gtagctggag ttgtaccagt cagcagattc cggtacgtca gtttgctcac
    25021 cccaggtttg cggagacgca ggaggcaggt cgcagtacca gtcgtagaag cttaagcaag
    25081 taccgccaat cagcgagaga tagcgcgcac ccgatgcgta agaaaccatc gacattgccg
    25141 gaattggcga gaagccagcg acacggtccg ggccgtaatt tttgatggtg taaacgttag
    25201 atgcggcgat cagttcgttc acttcctgcc aggaagaacg aacaaaacca ccgcgtccac
    25261 gcgcctgctt aaagcttttc gctttatcgg cgtcttcaat gatagaagcc catgcctcaa
    25321 ccggatcgct atgcagcgcc ttcgcttcac gccacatttt catcaggcgt ttgcgcatca
    25381 tcgggtattt caggcggtta gcgctgtaaa gataccagga gtagctggca ccgcgcgggc
    25441 agccgcgagg ttcatggttt ggcaggtccg ggcgagtacg cggatagtca gtctgctggg
    25501 tttcccaggt gaccagaccg tttttgacgt agattttcca gctgcaggag ccggtgcagt
    25561 ttaccccgtg ggtagagcgg acgattttgt catgctgcca acgctggcga tatccatcct
    25621 cccagtcacg gttggtattg agaagctggc catgcccatc ggcaaaggtt tcacccttct
    25681 gtttgaagta gcgaaaccgg tccaggaatt tactcatcgg ttttctcctg tgggagcctg
    25741 tcggccctct gataaatcga cattgctaat gtttaagggc aaggtaacgc tctgaaacag
    25801 cgtgggaatt g
//
LOCUS       DACADF010000066        25630 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.066, whole genome shotgun sequence.
ACCESSION   DACADF010000066 DACADF010000000
VERSION     DACADF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.066"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            116..589
                     /locus_tag="ISB30_002502"
     CDS             116..589
                     /locus_tag="ISB30_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP1990126.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCENYCGGIWNLYTLNNGGAFMAPEPDDYDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            605..1081
                     /locus_tag="ISB30_002503"
     CDS             605..1081
                     /locus_tag="ISB30_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAP1990127.1"
                     /translation="MQQISFLPGEMTTRERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1144..1365
                     /locus_tag="ISB30_002504"
     CDS             1144..1365
                     /locus_tag="ISB30_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAP1990128.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1384..2028
                     /locus_tag="ISB30_002505"
     CDS             1384..2028
                     /locus_tag="ISB30_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HAP1990129.1"
                     /translation="MSRIITTTVYTLHELSSTAQEKARDWYRQHHADSNWYENVYEDF
                     RAVCGIFGIDLRQRVFRLSNGRFMEEACIWFSGFCSQGDGACFEGRWHWQPATARRLR
                     EYATQDHELHRIADALQAVQKRNFWQLQAEIHHRGRYCHPYSMDITVTRNSPTGQAMT
                     ADAEAAVSEALRDLAFWLYRQLENEYDRLTSDAAVDEALLINEYTFTEAGLRAG"
     gene            2044..2412
                     /locus_tag="ISB30_002506"
     CDS             2044..2412
                     /locus_tag="ISB30_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAP1990130.1"
                     /translation="MSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            2501..2875
                     /gene="cbtA"
                     /locus_tag="ISB30_002507"
     CDS             2501..2875
                     /gene="cbtA"
                     /locus_tag="ISB30_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HAP1990131.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTSDNYRTVNNITLGKYPEAK"
     gene            2872..3066
                     /locus_tag="ISB30_002508"
     CDS             2872..3066
                     /locus_tag="ISB30_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990132.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            3079..3192
                     /locus_tag="ISB30_002509"
     CDS             3079..3192
                     /locus_tag="ISB30_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAP1990133.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(3481..3627)
                     /locus_tag="ISB30_002510"
                     /pseudo
     CDS             complement(3481..3627)
                     /locus_tag="ISB30_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            3681..3863
                     /locus_tag="ISB30_002511"
     CDS             3681..3863
                     /locus_tag="ISB30_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAP1990134.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVTSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(3964..4293)
                     /locus_tag="ISB30_002512"
     CDS             complement(3964..4293)
                     /locus_tag="ISB30_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAP1990135.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(4465..5523)
                     /locus_tag="ISB30_002513"
     CDS             complement(4465..5523)
                     /locus_tag="ISB30_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP1990136.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVLETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(5721..6194)
                     /gene="sbmC"
                     /locus_tag="ISB30_002514"
     CDS             complement(5721..6194)
                     /gene="sbmC"
                     /locus_tag="ISB30_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HAP1990137.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQMVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(6313..7479)
                     /gene="dacD"
                     /locus_tag="ISB30_002515"
     CDS             complement(6313..7479)
                     /gene="dacD"
                     /locus_tag="ISB30_002515"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HAP1990138.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            7688..9115
                     /gene="sbcB"
                     /locus_tag="ISB30_002516"
     CDS             7688..9115
                     /gene="sbcB"
                     /locus_tag="ISB30_002516"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAP1990139.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVILVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(9226..9684)
                     /gene="tnpA"
                     /locus_tag="ISB30_002517"
     CDS             complement(9226..9684)
                     /gene="tnpA"
                     /locus_tag="ISB30_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP1990140.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKCRAIGCILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMPYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            complement(9901..11259)
                     /gene="plaP"
                     /locus_tag="ISB30_002518"
     CDS             complement(9901..11259)
                     /gene="plaP"
                     /locus_tag="ISB30_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAP1990141.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQIVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(11249..11311)
                     /gene="yoeI"
                     /locus_tag="ISB30_002519"
     CDS             complement(11249..11311)
                     /gene="yoeI"
                     /locus_tag="ISB30_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAP1990142.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(11526..12455)
                     /locus_tag="ISB30_002520"
     CDS             complement(11526..12455)
                     /locus_tag="ISB30_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1990143.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(12501..13325)
                     /locus_tag="ISB30_002521"
     CDS             complement(12501..13325)
                     /locus_tag="ISB30_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1990144.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(13408..13662)
                     /gene="yoeB"
                     /locus_tag="ISB30_002522"
     CDS             complement(13408..13662)
                     /gene="yoeB"
                     /locus_tag="ISB30_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAP1990145.1"
                     /translation="MKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAACRYHY"
     gene            complement(13659..13910)
                     /gene="yefM"
                     /locus_tag="ISB30_002523"
     CDS             complement(13659..13910)
                     /gene="yefM"
                     /locus_tag="ISB30_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAP1990146.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKDIIE"
     gene            14193..14243
                     /gene="hisL"
                     /locus_tag="ISB30_002524"
     CDS             14193..14243
                     /gene="hisL"
                     /locus_tag="ISB30_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAP1990147.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            14389..15288
                     /gene="hisG"
                     /locus_tag="ISB30_002525"
     CDS             14389..15288
                     /gene="hisG"
                     /locus_tag="ISB30_002525"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAP1990148.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            15294..16598
                     /gene="hisD"
                     /locus_tag="ISB30_002526"
     CDS             15294..16598
                     /gene="hisD"
                     /locus_tag="ISB30_002526"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAP1990149.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            16595..17665
                     /gene="hisC"
                     /locus_tag="ISB30_002527"
     CDS             16595..17665
                     /gene="hisC"
                     /locus_tag="ISB30_002527"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAP1990150.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            17665..18732
                     /gene="hisB"
                     /locus_tag="ISB30_002528"
     CDS             17665..18732
                     /gene="hisB"
                     /locus_tag="ISB30_002528"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAP1990151.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGAQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            18732..19322
                     /gene="hisH"
                     /locus_tag="ISB30_002529"
     CDS             18732..19322
                     /gene="hisH"
                     /locus_tag="ISB30_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAP1990152.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIEED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGTAGAKLLKNFLEM"
     gene            19322..20059
                     /gene="hisA"
                     /locus_tag="ISB30_002530"
     CDS             19322..20059
                     /gene="hisA"
                     /locus_tag="ISB30_002530"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAP1990153.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVTGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVFLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            20041..20817
                     /gene="hisF"
                     /locus_tag="ISB30_002531"
     CDS             20041..20817
                     /gene="hisF"
                     /locus_tag="ISB30_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAP1990154.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            20811..21422
                     /locus_tag="ISB30_002532"
     CDS             20811..21422
                     /locus_tag="ISB30_002532"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAP1990155.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNILNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLATVIENLRKRHQ"
     gene            complement(21608..22585)
                     /gene="wzzB"
                     /locus_tag="ISB30_002533"
     CDS             complement(21608..22585)
                     /gene="wzzB"
                     /locus_tag="ISB30_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAP1990156.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQATPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSSYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGVGIVLGRNALRNYNAK"
     gene            complement(22731..23897)
                     /gene="ugd"
                     /locus_tag="ISB30_002534"
     CDS             complement(22731..23897)
                     /gene="ugd"
                     /locus_tag="ISB30_002534"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAP1990157.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGTIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(24144..25550)
                     /gene="gndA"
                     /locus_tag="ISB30_002535"
     CDS             complement(24144..25550)
                     /gene="gndA"
                     /locus_tag="ISB30_002535"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP1990158.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQRAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         6502 a         6645 c         6323 g         6160 t
ORIGIN      
        1 gccgaatatc attcatccct gatattaccg ggtctcacgg tcagtgatga acgtgatgtt
       61 gccagctccc gacctttact ccctctcatt tattcacatt acaaggattt catacatgaa
      121 aacattgtct cagaacacca cctcttcggc ctgtgcaccg gagactggcc tgcaacaact
      181 ggttgccacc atagtccctg atgaacagcg catcagcttc tggccgcagc attttggcct
      241 cattccacag tgggtgaccc tggagccccg tgtcttcggc tggatggacc gtctgtgcga
      301 aaactactgc ggtggtatct ggaatctgta caccctgaac aacggcgggg catttatggc
      361 ccctgaaccg gatgactatg atgacgaaac atgggtactg ttcaatgcca tgaacggtaa
      421 ccgcgctgaa atgagcccgg aagccgccgg cattgccgcc tgtctgatga cgtacagtca
      481 tcacgcctgc cgtacggagt gttatgccat gacggttcat tattaccgtc tgcgggatta
      541 cgccctgcag catccggaat gcagcgccat tatgcgcatt attgactgaa agggggccgg
      601 aacaatgcaa cagatttcct ttctgcccgg agaaatgaca accagagagc gcagcctcat
      661 tcagcgggcc ctgaaaaccc tggaccgcca tcttcatgaa cccggcgtgg ccttcacctc
      721 cacccgtgcg gcacgggaat ggctgattct gaacatggcg ggactggagc gtgaagaatt
      781 ccgggtgctg tatctgaata accagaatca gctgattgcc ggtgaaaccc tcttcaccgg
      841 caccatcaac cgcacggaag tccatccccg ggaagtgatt aaacgcgccc tgtatcacaa
      901 tgccgcagcc gtggtgctgg cgcacaatca cccgtccggt gaagtcacac ccagtaaggc
      961 agaccggctt atcaccgaac gtctggtaca ggcactgggc ctggtggata tccgggtgcc
     1021 ggaccatctg atagtcggtg gcagccaggt tttctccttt gccgaacatg gtctgcttta
     1081 acccgtcaca accacatcac acctgttttc acttttatct tctgtcttca gaggtatccc
     1141 actatgaaaa tcatcacccg tggcgaagcc atgcgtattc accgtcagca tcctgcatcc
     1201 cgtctttttc cgttctgtac cggcaaatac cgctggcacg gcagtgctga agcgtatacc
     1261 ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt ttgctgaacg ccgtaaggac
     1321 agttttggcc cgtatgtccg gctgatgagc gtcactctga actgagtcag taagggcatt
     1381 ctgatgagca gaattatcac caccaccgtg tatacactgc atgagctatc ctccaccgca
     1441 caggaaaaag cccgcgactg gtaccgtcag catcatgctg acagcaactg gtatgaaaac
     1501 gtgtatgagg acttcagggc ggtctgtggc attttcggaa ttgacctccg ccagcgcgtt
     1561 ttccggctca gcaacggcag gtttatggaa gaggcgtgca tctggttcag cggattctgc
     1621 agccaggggg acggagcctg ttttgaagga cgctggcact ggcagccagc aacagccaga
     1681 aggctccggg agtatgccac acaggaccat gaactccatc gtatcgcgga tgccctgcag
     1741 gcggtgcaga aacgtaactt ctggcaactg caggctgaaa tccaccaccg gggacgttac
     1801 tgccatccgt acagcatgga tatcactgtt acccggaaca gtccgactgg tcaggctatg
     1861 acagccgatg cggaagccgc tgtcagcgaa gccctgcgcg acctggcctt ctggttgtac
     1921 cgtcagctgg aaaacgagta cgacaggctg acctctgatg ccgctgtgga tgaggccctt
     1981 ctcatcaacg aatacacctt caccgaagcc ggtctgcgcg ccggctgacc tgaggacttt
     2041 atcgtgtcag acaaactcca tgagacaaat tatcccgacg accataacga ccgcctctgg
     2101 tggggactgc cctgcaccgt gacgccctgt ttcggggcac gtctggtgca ggagggtaac
     2161 cggttgcatt accttgccga ccgcgccggt atcagaggcc tgttcagcga tgcagatgcg
     2221 taccacctgg accaggcctt tccgctgctg atgaaacaac tggaactcat gctcaccagc
     2281 ggtgaactga atccccgcca tcagcatacc gtcacgctgt atgcaaaagg gctgacctgc
     2341 aaagccgata ccctcagcag ttgtggttac gtttatctgg ctgtttatcc gacgcccgaa
     2401 atgaaaaatt aactctccag aatagccttc tgctacggcc tggtgttttc accacgccac
     2461 ttttccattt ttatatctgc atatcaggaa aatcttcagt atgaaaacat tacctgtatt
     2521 acccgggcag gcggccagtt ctcgcccgtc tcctgttgaa atctggcaga tactgctgtc
     2581 ccgactgctg gaccagcact atggcctcac actgaatgac acaccgtttg ccgatgaacg
     2641 tgtgattgag cagcatattg aggcaggcat ttcactgtgt gatgcggtga actttctcgt
     2701 ggaaaaatac gcgctggtgc gtaccgacca gccgggattc agcgcctgta cccgctctca
     2761 gttaataaac agcatcgata tcctccgggc tcgcagggcg accggcctga tgaccagcga
     2821 caattacaga acggtaaata acattaccct gggtaagtat ccggaggcga aatgatgaca
     2881 ctggaagccg acagcgttaa cgtacaggcg ctggatatgg ggcacattgt cgttgacatt
     2941 gatggtgtta atatcactga actgattaat aaggccgctg aaaacggtta ttcactccgc
     3001 gtggtggatg accgtgactc taccgaaaca ccggcaactt atgccagccc tcaccagttg
     3061 ctgtaagaca atgcagtgat acagggtata gtgccattaa ccacagaaac agcactgttg
     3121 tcctgtatta agcaggcaag agaagatgtc cttaccctgc gccatctgca gcttctgcac
     3181 cagaaccggt gaatctcact caacaggctt catttcccct gacgccagcc tgaatacagc
     3241 tggcgttttc atttataaac agaaaggaaa accgataata atggaatgga cgccccgacc
     3301 atgaagcaac ggtaggatat tactgataaa ccttccagtg gaggtgcgtt atggccgttt
     3361 taacgattag cattgacctg acaaaaaatg aattccaaat ccacggtctg ggtaggaaca
     3421 gaaaaatcta agctcagaaa acggattaaa gtaagcgtca acggagcacc gtattgacgc
     3481 ttatttattg gtgagaacta cgttccatgg caggagttcg tcaacacggt tggagggcca
     3541 ttccggcagt atgctcagaa tatggcgcag atacgcttcc ggatcgatac cgttcagacg
     3601 gcaggtgcct gcttttccgg caacaagcag gaatctcagc gctgaagcag gaattagagg
     3661 taaaaacgcc ctaccgggcc atgaatcacc cggtcattgg agtagtaacc aaagcagatt
     3721 tagccagcat ggaacaaata tctttggtga caagttggtt acgggaagct ggagcgcaca
     3781 acgtgttagt aaccagtgca gttaataaca atggagttac agaacttttt gccttgctgc
     3841 atacagaaga aggctgttgt taatttcgat tggttctcag atgagaatga tgacggtgat
     3901 acacagataa gaatcaatga gcaatgaggg ttgccgggca accctcattg aataaaacgg
     3961 gaattacttc gcttcgccgt ttttcatttc gcccatagct ttcagctttt tggagatatc
     4021 gcggcgttct ttggagagct cggcattttt gatgatgtaa tcgtcaacgc gatcttcata
     4081 gtcacctttc atgctggcga tgatgccctg gattgcttca acgctcatcc ctggcttgat
     4141 gtaatcgctc aggttgtcca gcaacaggac gcgcttctgg ttgtcacgga tctttttctc
     4201 aacgtcctga atttcacgtt gcagtttgtt cttacgacgg aacagacgaa caaattccag
     4261 tacgtcctgg aatgaaggct tggtagtttc catttttata cccctgataa tgtgagagtc
     4321 ggattcgttt aagcaaccgc tattcgttag ggccaacatt actcatagct gccgcagatg
     4381 acaatgcttt tatccctttt cactatcata ccctttatcc ttgctgaatc gaagcagcag
     4441 caagatgatt ctgaagttca ggaattattt gcgcaaggcc cggcaaatca gattcgatag
     4501 caactcaagc tgatgtgccg tttccatgtt cagccacaca taaccataga ttggtgtttc
     4561 caagactttc aggtcatggt ggttatcgag caactgacgc agctcttcca cagcatcgtt
     4621 caatttttcc gtattggcaa aaaccggctg cggattacct tcgtacagcg catgaacaag
     4681 gctcagcagt atttgctgca tcatgtgctg ggtatcacga agtttttgcg cgttcaataa
     4741 cacgaaatgg ctggggcgcg tggcccagta tgcattgatt tgcaactcca gcatacaaac
     4801 cagattgcga ttaatggtct ggataccttc atatatcgat ttgggaatac gggtttcttt
     4861 gctggcgggc gcaatcagcc cacgcatttt cacggcatcg gtcaggagtt tttgtagatg
     4921 gctttccaga cgtgggcgtt cgagtaagtt cggtgagaat gcagattgat agacccgatt
     4981 atactcggtc agacttttcg ccagttgaat gcgccagtgg atgaacgccc gttgtggcca
     5041 gataccggta aacaacattg ccagtaaaga gccgaggatc acatcgccgc ttcgccataa
     5101 cgccgtatca atttcacctg tcggggaacc cacaacaatt gccagcgtca ccccaatcaa
     5161 taatccttga tacggtttct tgcccagcgc cagccaaccg caaagaaaca tcgccgccgc
     5221 gcaccagact aacatcagcg gtaacgaaat taactccagt tgcagagcga taagacctaa
     5281 aatcgaaccc aacaccgtac cgccaatacg ttcgaaggca cgagggacaa cgttccccca
     5341 gaacgagatt ggccccataa tcaccaccat ggtgaccagc ggccaggtgc tttccgggat
     5401 agtaaacagg cggataatga gaaaagtgag caggaatgcc agcgcgaccc gagtaccatg
     5461 cacaatgcgg taatggcggt atacccggat ttcaaacggg cttaatgact tatcggcacg
     5521 cacgcagaaa ctccgtaatg aagatgaaaa gcgctcataa cacatctgtc ggccagaagg
     5581 cgcgcctctg agttatccga cggacatacc gggaacagtg taatttcccg gcaggagcga
     5641 aaaatttgaa aggcgcatct tactcttttc gctttcaaaa aaagagtggt cttcgcgtta
     5701 acacaccgcc ctgagatgaa ttagtgatgt tttggctgca ccgcaacata catttcgata
     5761 tcccagtacc catcttccgc gccattgttc aagtaaacct cgaagcaggg ctttggtaac
     5821 atttcataag cactgtcctg caagaggcta ttaaagaact gataccaggg tttagcaaaa
     5881 tcatcaccga ctacacgagc caccgccacc gcatactgac cacctgaaat ttctgtcaga
     5941 atgacgccct cactgttttc ggggagcgta aagttattcg gtaccgtcac gacggtgtcg
     6001 cagcgtaatt tttcggcggg tgtttcatct ggattgtcgt aatagacagc aacccactcc
     6061 ttcggcacaa tatttttgct atctatccac atcatcaact gctcaaaacc tttctttacc
     6121 atctgctccc acgggccaac aagatggaaa cctgcaacgg tacgtttatc ttcctgctta
     6181 atctcgtagt tcatgacgcc tccactaaca ctgtttttat atacagtata gttgcaatat
     6241 taaaaccaca aggaatgagt gttgattatg cgagcagact cgcactcctg ccagtctgct
     6301 gcaaaagaaa ggtcaggcct tatggtggaa ataatcactc aggcgggaaa acatgctgcc
     6361 ttccccgaca gattccaggg taaccagcgg ccagtgcgcc acctgtttat cacggtcgta
     6421 aagttcaatt tcccctaccc gctgatgggc gctaattggc gcggtgagtt ctttaccatc
     6481 aagggtatat ttggctttga tatgtggaat ttcggctttc ggtagcacca tccagaactc
     6541 ttgttccgtt cccagggcga tattttcttt atcaccatac cagatgcgtt ccgtaccgac
     6601 ctttttccca cggtgcaaaa tttgcaccgt agtaaagttt tgttgccccc aacgcagtaa
     6661 ttttcttgcc tcttcctcac gaccttttgc gctgtcagcc cccattacca ctgcaatgag
     6721 gcgacgctgc ccgtctacag ccgaagcaat aagattaaac ccggcaccag aagtatggcc
     6781 cgttttcagg ccgtcaacat tcatggtttt atcccacaat aacccgttac ggttttgctg
     6841 ggtgataccg ttccaggtga ggcttttctc actgtacata tgataaaact cgggctcgcc
     6901 gtggatgata gcgcgagaaa gcacggctaa atcataagcc gagctatgct ggccaggtgc
     6961 atccagacca tgcactgttt caaaatgcgt atccttgaga tgcagcttct cggcatagtt
     7021 gttcatcatt tcaacaaact gccgttgccc accggcaata tagtcagcca gagcaacaca
     7081 agcgtcattt ccggaatcca caattaaacc acggcttaaa tcacgtaccg atacgcgatc
     7141 gccctctttc aaaaacatca gtgaagaacc gacaaacacc ggattatctt tcgcccacgc
     7201 atcacgcccc acggtgacaa tatcgtctgg cgtaatgcga tgactatcga tagcgcgatc
     7261 cacgacatag cccgtcatca gctttgtcag gctggcggga ttgcgctgtt gatgctcatt
     7321 acccgcggtg agaatctgac cggtggtgta atccatcaat acccaggacc cggcatgaat
     7381 ctctggaggc tgaggtgaaa aaggaatgtt ttccgccgca aaaccagacg ataaattaaa
     7441 aacgaacaaa gaagcagcaa taataagacg gcgtttcaac agcaaaccct caggtgtttc
     7501 aaatagctgt tctttttacg gaaatactta tgaactggct ggaataaagt gcaagaaaat
     7561 gtgactacta tctcattttt atctgacatg atctgttgcc actcgctgcc aaattgtcgc
     7621 gctaaagctg attagcacgg tgatatttga tactctggca gacagcagaa ataacggatt
     7681 taacctaatg atgaatgacg gtaagcaaca atctaccttt ttgtttcacg attacgaaac
     7741 ctttggaacg caccccgcgt tagatcgccc tgcacagttc gcagccattc gcactgataa
     7801 cgaattcaat gtcatcggcg aacccgaagt cttttactgc aagcccgcgg atgactattt
     7861 accccagcct ggagcagtat taattaccgg tattaccccg caggaggcac gggcgaaagg
     7921 agaaaacgaa gccgcgtttg ccgcccgtat tcactcgctt tttaccgtac cgaagacctg
     7981 tattctgggc tacaacaatg tgcgtttcga cgacgaagtc acacgcaacg ttttttatcg
     8041 taatttctac gatccttacg cctggagctg gcagcatgat aactcgcgct gggatttact
     8101 ggatgttatg cgtgcctgtt atgccctgcg cccggaagga ataaactggc ctgaaaatga
     8161 tgacggtcta ccgagctttc gccttgagca tttaaccaaa gcgaatggta ttgaacatag
     8221 caacgcccac gatgcgatgg ctgatgtgta cgccactatt gcgatggcga aactggtaaa
     8281 aacgcgtcag ccacgcctgt ttgattatct ctttacccat cgtaataaac acaaactgat
     8341 ggcgttgatt gatgttccgc agatgaaacc cctagttcac gtttccggaa tgtttggggc
     8401 atggcgtggc aataccagct gggtggcacc gctggcgtgg catcctgaaa atcgcaatgc
     8461 cgtaattttg gtggatttgg caggagacat ttcgccatta ctggagctgg atagcgacac
     8521 attgcgcgag cgtttatata ccgcaaaagc cgatcttggc gataacgccg ccgttccggt
     8581 taagctggtg catatcaata aatgtccggt actggcccag gcgaatacgc tacgcccgga
     8641 agatgccgac cgactgggaa ttaatcgtca gcattgcctc gataacctaa aaattctgcg
     8701 tgaaaatccg caagtgcgtg aaaaagtggt ggcgatattc gcggaagccg aaccgtttac
     8761 accttcagat aacgtggatg cacagcttta taatggcttt ttcagtgacg ccgatcgtgc
     8821 agcaatgaaa attgtgctgg aaaccgagcc gcgtaattta ccggcactgg atatcacttt
     8881 tgttgataaa cgaattgaaa aactgttgtt caattatcga gcgcgcaact tcccggggac
     8941 gctggattat gccgagcagc aacgctggct ggagcaccgc cgccaggtct tcacgccaga
     9001 gtttttgcag ggctatgctg atgaattgca gatgctggca caacagtatg ccgatgacaa
     9061 agagaaagtg gcgctgttaa aagcgctttg gcagtacgcg gaagagatcg tctgaaaata
     9121 aatatcctcc ggcatagccg gaggtttttc aaatgcgcct ttaaggctct cttaccagcc
     9181 gcgccctaac aggcgcacac gatctgacat ttgcatccaa cttcgttact tacggcccgt
     9241 aaacgggctg cccggatagg gaatcgataa ctgctctccc attttatcct cttcaagctg
     9301 gtgctttatg taatcctgta tcttcgccgt gttcttaccc accgtatcga cgtaataccc
     9361 tctgcaccag aactccctgt tcctgtattt gaatttcaaa tcaccaaact gctcgtaagg
     9421 catcagactg cttttccctt tcagatatcc cataaagcct gatacgctca ttttgggcgg
     9481 gatctccaca agcatatgga tatgatctgc acagcattca gcttccagaa tccgtacact
     9541 tttccactca cacagctttc tcaaaataca gcctattgct ctacacttct ctctgtagaa
     9601 cacctgtctt cggtattttg gcgcaaatac tatgtgatat ttacagttcc atcgggtgtg
     9661 cgctaagctc ttttcgttcc ccattgggac ccccttttga tttcttgttt gacacttgca
     9721 gttgccagac cgcaaggtgt tttaacaaat caaaaggggt tttaataact ggctcaaagc
     9781 tgaaagcttt ccggaacccc cagcctagct gggggttttc tgtgcacaac aaaaaagccg
     9841 gagcgacaaa ctccggcttt ttgagattta ccggatgatt atgtcatccg gtttgtaaga
     9901 ttacgctacg tcttcgtact gcggaaccgg gttacggaaa ctcttggtta cgcaagccag
     9961 gtaaatcaga ccgatagcag cccagatcag acccagaacc attgagcttt cttccaggtt
    10021 aacccacagc gcaccaacgg tcagcgcacc acacatcggc aggaacagat actggaagtg
    10081 atctttcagc gttttgttac gcttctcacg gatccagaac tgcgagatga ccgacaggtt
    10141 aacgaaggtg aacgccacca acgcaccaaa gttaatcagc gccgtagcca ttaccaggtc
    10201 gaagttgatt gccagcaggg caatcgcgcc aaccaggatg atattcatcg ccggagtacg
    10261 ccatttcggg tgtacataac cgaagaagct tttcgggaac acgccgtcac gacccattac
    10321 gtacatcaga cgcgcaacgc ctgcgtgcgc cgccataccg gacgccagta cggtaatggt
    10381 ggagaagatc agcgcgccaa cctggaacgc tttacccgca acgtacagca tgatttcagg
    10441 ctgtgacgca tccggatctt taaaacgaga gatatccggg aagtacagct gcaggaagta
    10501 agttgcaaag atgaagatca tgccgccgat cagcgcggtc aggaaaatcg cacgcgggat
    10561 cacgcgctct gcatctttgg tttcttccga caggttgctg atgccgtcaa aaccggtaaa
    10621 ggagaagcac aggattgtcg ccccggtaat catcgggata acatgcgcat cgccagacca
    10681 gaacggacga gtgctcgcca gcgtaccagc gccttcacct tcaaatacgc cataaacaac
    10741 catgcccaga atcaccgcga tcagcactat ctgcaacacg acgattacag tgttgaagtt
    10801 cgctacggat ttcagactac gcaggttaaa ggcggtcata aaggccacca gagccaccac
    10861 aaacatccac gatggaatgg aaggcaccag agcttcaaaa tagattttcg ccagcagaat
    10921 gttgatcatc ggcgcgaaca gatagtcgag cagagaagac caacccacca taaagccgac
    10981 agtcgggcta atggatttct gggcgtaagt gtatgcagag ccagcagaag gatagcggcg
    11041 aaccagcttc ccgtagctca gagccgtaaa caggatcgca atcaatgcga acgcataggc
    11101 tgtcggcaca tgaccatccg tgaggcctga aacgatacca aatgtatcaa acagcgtcat
    11161 cggctgcata taggctagac ccatcattac aaccggaact aacgtaagcg ttttacgcaa
    11221 ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg gtcattctcc tttacggtga
    11281 taaccgtcgc gtaagcaaaa aattgcccca tttttttgga ttcctcagcg acaacaactg
    11341 tcgattttta gtaaatatct atccggtacg aagcccggcc tcttggtatg aatgattggt
    11401 ttgaagcaaa aaataaccga cgctaattaa acgtcggttt ttagtcattt tttgacagcg
    11461 gcgcattgtg cctaaatggg ggggaaatga caagagaatg agaggcttgc cataataatt
    11521 tttctttaaa tggctgattt tccgtcatca gattttgcat aaacaccggc gatcgcacta
    11581 tttgctaaaa tttcatctcg ccaccagccg ctggcaagcc ctgcggtctg ttcattccag
    11641 ccaatccaga cgggttcaaa gagagtttgg gcgaccactt tcttctcgat taacgcgcca
    11701 ctatcgagaa aacgttgcgc cagataccgt ggtaaatagc cgcagccaag gccgctaatt
    11761 tgcagctcca gcttggtttt aaaatcgaaa acggtaatgg cttcttgttc gtcgagaagt
    11821 tgtgaggccg tagaagcgcc tgcctgcgca gtgtccccca ccacaatcgc ccgatatcgt
    11881 ttaatgatac gacggtttaa cggttcttct tcctgggcta acggatgatg aggcgcgacc
    11941 gcaaagactt gttcaagatc gcccaaccgc gagaaaccaa actcactgga agatggtggc
    12001 tcatgcatcg cgccaacgat aatatccgct cgcccctgag tcagggcatc ccaggaacca
    12061 ccgagtacgc cattgataaa cttcaggcgc gtcacgctat gatgttgata aaaagcttca
    12121 atgagaggcg caagcagaga aaaaggaaac gtgtcgtcca cgccaatcac cagctcgttt
    12181 tcccagcctt catgcagttt gatcgcctgc ttttccagtt ctcgcacggt atgcagaact
    12241 tcccgccctt tctctaataa catttttccg gtacgggtga atttagcgcg gtgtccgcta
    12301 cgatcaagca attgaatatt aaggtcgctt tccagcttgt gaacggtgta actcagagcg
    12361 gacggcgtct tgtagagttt ggctgacgcc gccgcaaaac tgccttcttt ttctaaggca
    12421 tcgaggatca tcaacacatc cagcagtggt ttcatacacg cccccttgca gtgcatgcgg
    12481 cgtcctgctg gcgtgatcgt ttactccagc ggcattacca gcggatcggg atactggtat
    12541 tcaaatccca gttcattaca aatccgactg ccatcaataa tcttaccttt gccgctgtcc
    12601 agactatttc tgaactgcgg aggttccagc cccagtaaac gggccatctg cggatagaaa
    12661 acattacgcg cagggtgagc tggcgcacat atattataga tgtgtccgcc tttaggtgcc
    12721 tgtaacaaca gagtgatagc gccaataaca tcttctaaat ggactaaatt aacaccatgt
    12781 tcaccatcag gggcggtttt tccggcaaag aagcgtccgg gatgacgtcc cggtccgacc
    12841 aggcccgcaa gacgcagaat atcgaccgaa gtaccgggta aattgtgcag ccagtcttcg
    12901 agttcttcta atactcgtcc actgttggtt accggattac gcggggtggt ttctttcacc
    12961 gtgccttgtg cgtcgccata gacagacgtc gagctggtaa aaataatacg gggaatacga
    13021 tgagccagcg cgctatccac taactcttgt accgcttgta aatagaactc atcgccgggg
    13081 ccgctacgac gtgccggaag cgtaatgacc agcgcatcgg catccataag ggcatccaga
    13141 tcgtcagaat cgcaaactaa ctcaggctcc atgcgaagca gatagctatc aatgccactc
    13201 attcgggccg cttcgacacc atcttgtgtg gttttactcc cggtgacttg ccagcctcgc
    13261 gctgaaagtg acatcgccag cggcatacct aaccacccta acccgacaat tgcgaccttt
    13321 ttcatccgtt atctcctgat acaccttaga tctataaggc tacgctagcg tatcaaaact
    13381 gacaattcat tctatgaatg aatctcttca ataatgataa cgacatgctg caatgagcag
    13441 tgaatcatca gtaaccgcgt ataccagaag gtgctcctct gtaatgcgtc gggaccagaa
    13501 acctgacaag ttatgtttca ggggttctgg tttcccctta ccttcaaatg gtgttctgcg
    13561 ggtatctttg ataagttcat tgatcttttt aacaattcgc ttatctgctt cctgccagta
    13621 cagataatca tcccatgatt cctcagacca gattagtttc actcaataat gtccttttcc
    13681 gttcctttgt ctgatttcag gctatcgatt gagtccatca atctccgggc gttagcgggg
    13741 gaacgcagta gataagccgt ctcttccagc gagttgtatt cttcgagtga catcagaaca
    13801 caagcctctc cattctgacg agtaatgagg atcggggcat gatcttcaac ggctttcatc
    13861 attgttgccg acaaattctg acgcgcttcg ctgtagctaa ttgtacgcat gtaaatctcc
    13921 tcttttgtac agttcattgt acaatgatgg gtgttaatta actatttatt aattagtttg
    13981 tagatcaaga cattgccagc gagacgaaaa atcagacttt gctctttttg gtgctgtcaa
    14041 gttagaggac agtcctctta gcccccttct ttccccgctc attcattaaa caaatctatc
    14101 gccataaaat atataaaaaa gcccttgctt tctaacgtga aagtggttta ggttaaaaga
    14161 catcagttga ataaacattc acagagactt ttatgacacg cgttcaattt aaacaccacc
    14221 atcatcacca tcatcctgac tagtctttca ggcgatgtgt gctggaagac attcagatct
    14281 tccagtggcg catgaacgca tgagaaagcc cccggaagat caccttccgg gggctttttt
    14341 attgcacgat tgataacggt tcagacaggt ttaaagagga ataagaaaat gacagacaac
    14401 actcgtttac gcatagctat gcagaaatcc ggccgtttaa gtgatgactc acgcgaatta
    14461 ctggcgcgct gtggcattaa aatcaatctt cacacccagc gtctgatcgc gatggcagaa
    14521 aacatgccga ttgatattct gcgcgtgcgt gacgacgata ttcccggtct ggtaatggac
    14581 ggcgtggtag accttgggat tattggcgaa aacgtgctgg aagaagagct gcttaaccgc
    14641 cgcgcccagg gtgaagatcc gcgctacttt accctgcgtc gtctggattt cggcggctgc
    14701 cgcctttcgc tggcaacgcc ggttgatgaa gcctgggacg gtccgctctc cttaaacggt
    14761 aaacgtatcg ccacctctta tcctcacctg ctcaagcgtt atctcgacca gaaaggtatc
    14821 tcttttaaat cctgcttact gaacggttct gtcgaagttg cgccgcgtgc cgggctggcg
    14881 gatgcgattt gcgatctggt ttccaccggt gccacgctgg aagctaacgg cctgcgcgaa
    14941 gtcgaagtta tctaccgctc gaaagcctgc ctgatccagc gcgatggcga aatggaagaa
    15001 tccaaacagc aactgatcga caaactgctg acccgtattc agggtgtgat ccaggcgcgc
    15061 gaatcaaaat acatcatgat gcacgcaccg accgaacgcc tggatgaagt catcgccctg
    15121 ctgccaggtg ccgaacgccc aaccattctg ccgctggcgg gtgaccaaca gcgcgtagcg
    15181 atgcacatgg tgagtagcga aaccctgttc tgggaaacga tggaaaaact gaaagcgctg
    15241 ggtgccagtt caatcctggt cctgccgatt gagaagatga tggagtgatc gccatgagct
    15301 ttaacacaat cattgactgg aatagctgta ctgcggagca acaacgccag ctgttaatgc
    15361 gcccggcgat ctccgcttct gaaagcatta cccgcaccgt taacgatatt ctcgataacg
    15421 tgaaaacgcg tggcgatgag gccctgcgtg aatacagcgc gaagtttgat aaaaccacgg
    15481 ttaccgcgct gaaggtgtct gctgatgaga tcgccgccgc cagcgaacgc ctgagcgacg
    15541 agctaaaaca ggcgatggcg gtggcagtaa agaatattga aaccttccac actgcgcaaa
    15601 aactgccgcc ggtagatgta gaaacgcagc caggcgtgcg ttgccagcag gtcacgcgcc
    15661 cggtggcttc agtgggtttg tatattcctg gcggctccgc cccgctcttc tcaacggtat
    15721 taatgctggc gactccggcg cgtattgcgg gctgtaaaaa agtggtgctg tgctcaccgc
    15781 cgccgattgc cgatgagatc ctttatgcgg cgcaactgtg cggtgtgcag gacgtgttta
    15841 acgtcggcgg cgcacaggcc attgccgcgc tggcgtttgg tacggaatct gtgccgaaag
    15901 tggacaaaat cttcgggccg ggtaacgcct ttgtcaccga agcaaaacgc caggtaagcc
    15961 agcgtctgga cggtgcggcg atcgatatgc ccgcaggccc gtcggaagtg ctggtgattg
    16021 ctgacagcgg cgcaacgccg gatttcgtgg cttctgattt gctctcccag gctgaacacg
    16081 gcccggactc acaggtgatt ttactgacac ctgatgctga tatggcgcgt cgcgttgccg
    16141 aggccgtcga acgccagttg gcagaattgc cgagagctga aaccgcccgt caggcactga
    16201 gcgccagccg cctgatcgta accaacgatt tagcgcagtg cgtagagatc tccaatcagt
    16261 acggtccgga gcacctgatc attcagaccc gcaacgcccg cgatctcgtc gatagcatta
    16321 ccagcgccgg ttcggtattt ctcggtgact ggtcaccgga atcggcaggt gattacgcct
    16381 ccggcaccaa ccacgtgctg ccgacttacg gttacaccgc cacctgttcc agcctcgggc
    16441 tggcagattt ccagaaacgc atgaccgtac aggaactgtc gaaagaaggt ttctccgcgc
    16501 tggcttcgac cattgaaaca ctggccgccg ccgagcgcct gaccgcccac aaaaatgccg
    16561 ttactttgcg tgttaacgtc cttaaggagc aagcatgagc accgtgacta ttactgattt
    16621 agcgcgtgaa aacgtccgca acctgacgcc gtatcagtcg gcgcgtcgtc tgggcggtaa
    16681 cggcgacgtc tggctgaacg ccaacgaata tcccacagcc gtggagtttc agcttactca
    16741 gcaaacgctc aaccgctacc cggaatgcca gccaaaagcg gtgattgaaa attacgcgca
    16801 atatgcaggc gtaaaaccgg aacaggtgct ggtcagccgt ggcgcggacg aaggtattga
    16861 actgctgatc cgcgcttttt gcgaaccggg taaagacgcc atcctctact gcccgccaac
    16921 gtacggcatg tacagcgtca gcgctgaaac cattggcgtc gaatgccgca cagtgccgac
    16981 gctggaaaac tggcaactgg acttacaggg catttccgac aagctggacg gcgtaaaagt
    17041 ggtctatgtt tgcagcccca acaaccccac cgggcaactg atcaacccac aggattttcg
    17101 caccctgctg gagttaaccc gcggtaaggc gattgtggtt gccgatgaag cctatatcga
    17161 gttttgcccg caggcatcgc tggctggctg gctggcggaa tatccgcacc tggctatttt
    17221 gcgcacactg tcgaaagctt ttgctctggc ggggcttcgt tgcggattta cgctggcaaa
    17281 cgaagaagtc atcaacctgc tgatgaaagt gatcgccccc tacccgctct cgacgccagt
    17341 tgccgacatt gccgcacagg cgttaagccc gcagggaatc gtcgccatgc gcgaacgggt
    17401 ggcgcaaatt attgcagaac gcgaatacct gattgccgca ctgaaagaga tcccctgcgt
    17461 ggagcaggtt ttcgactctg aaaccaacta cattctggcg cgctttaaag cctccagtgc
    17521 agtgtttaaa tctttgtggg atcagggcat tatcttacgt gatcagaata aacaaccctc
    17581 tttaagcggc tgcctgcgaa ttaccgtcgg aacccgtgaa gaaagccagc gcgtcattga
    17641 cgccttacgt gcggagcaag tttgatgagt cagaagtatc tttttatcga tcgcgatgga
    17701 accctgatta gcgaaccgcc gagtgatttt caggtggacc gttttgacaa actcgccttt
    17761 gaacctggcg tgatcccgga gctgctgaag ctgcaaaaag cgggctataa gctggtgatg
    17821 atcaccaatc aggatggtct gggagcacaa agtttcccgc aggcggattt cgatggcccg
    17881 cacaacctga tgatgcagat cttcacctcg caaggcgtgc agtttgatga agtgctgatt
    17941 tgcccacacc tgcccgccga tgagtgcgac tgccgtaagc cgaaagtaaa actggtggag
    18001 cgttatctgg ctgagcaagc gatggatcgc gctaacagtt atgtgattgg cgatcgcgcg
    18061 accgacattc aactggcgga aaacatgggc attaatggtt tacgctacga ccgcgaagcc
    18121 ctgaactggc caatgattgg cgagcaactc actaaacgag accgttacgc ccatgtagtg
    18181 cgcaacacca aagagacgca aattgacgtc caggtgtggc tggatcgcga gggtggcagc
    18241 aagattaaca ccggcgttgg cttctttgat cacatgctgg atcagatcgc cacccacggc
    18301 ggtttccgta tggaaatcaa cgttaaaggc gacctctata tcgacgatca tcacaccgtc
    18361 gaagataccg gcctggcgct gggggaagca ttaaaaattg ctcttggcga caaacgcggt
    18421 atttgccggt ttggttttgt gctgccgatg gacgaatgcc ttgcccgctg cgcactggat
    18481 atctctggtc gcccgcacct ggaatataaa gccgagttta cctaccagcg tgtgggcgat
    18541 ctcagcaccg agatgatcga gcacttcttc cgttcgctct catacaccat gggcgtgacg
    18601 ctacacctga aaaccaaagg taaaaacgat caccaccgtg tagagagcct gttcaaagcg
    18661 tttggccgca ccctgcgcca ggccatccgc gtggaaggcg acaccctgcc ctcgtcgaaa
    18721 ggagtgctgt aatgaacgtg gtgatccttg atactggctg cgccaacctg aactcggtga
    18781 agtctgccat tgcgcgtcac ggttatgaac ccaaagtcag ccgtgacccg gacgtcgtgt
    18841 tgctggccga taaactgttt ttacccggcg ttggcaccgc acaagcggcg atggaccagg
    18901 tgcgcgagcg cgagctgttt gatctcatca aagcctgtac ccaaccggtg ctgggcatct
    18961 gcttagggat gcaactgctg gggcggcgca gcgaagagag caacggcgtc gacttgctgg
    19021 gcatcatcga agaagacgtg ccgaaaatga ccgactttgg tctgccactg ccacatatgg
    19081 gctggaaccg cgtttacccg caggcaggca accgcctgtt tcaggggatt gaagacggcg
    19141 cgtactttta ctttgttcac agctacgcaa tgccagtcaa tccgtggacc atcgcccagt
    19201 gtaattacgg cgaaccgttc accgcggcgg tacaaaaaga taacttctac ggcgtgcagt
    19261 tccacccgga gcgttctggt accgctggcg ctaagttgct gaaaaacttc ctggagatgt
    19321 gatgattatt ccggcattag atttaatcga cggcaccgtg gtgcgtctcc atcagggcga
    19381 ttatggcaaa cagcgcgatt acggtaacga cccgctgccg cgcttacagg attacgccgc
    19441 acaaggtgcc gaagtactgc acctggtgga tctgaccggg gcaaaagatc cggctaaacg
    19501 tcaaatcccg ctgattaaaa ccctggtcac gggcgttaac gtcccggtgc aggttggcgg
    19561 cggcgtgcgt agcgaagaag acgtggcggc gttactggaa gcaggcgttg cgcgtgtggt
    19621 ggttggctcc accgcggtga aatcaccaga aatggtgaag ggctggtttg aacgttttgg
    19681 tgccgatgcc ttagtgctgg cgctggatgt ccgtatcgac gagcaaggca acaagcaggt
    19741 ggcagtcagc ggctggcaag agaactcggg cgtttcactg gaacaactgg tggaaaccta
    19801 tctgcccgtc ggcctgaaac atgtgctgtg taccgatatc tcgcgcgacg gcacgctggc
    19861 gggatctaac gtctttttat atgaagaagt gtgtgccaga tatccgcaag tggcgtttca
    19921 gtcctccggc ggcattggcg atattaacga tgtggcggcc ctgcgtggta ctggtgtgcg
    19981 cggcgtaata gttggtcgtg cattactgga aggtaaattc accgtgaagg aggccatcgc
    20041 atgctggcaa aacgcataat cccgtgtctc gacgttcgtg atggtcaggt ggtgaaaggc
    20101 gtacagtttc gcaaccatga aatcattggc gatatcgtgc cactggcaaa acgctacgcc
    20161 gaagaaggtg cagatgaact ggtgttctac gatatcaccg cttccagcga tggccgtgtg
    20221 gtagataaaa gctgggtatc tcgcgtggcg gaagtaatcg acattccgtt ttgtgtggcg
    20281 ggtgggatta agtctctgga agatgccgcg aaaattcttt cctttggcgc agataaaatc
    20341 tccatcaact ctcctgcgct ggcggacccg acgttaatta ctcgcctggc cgatcgcttt
    20401 ggcgtgcagt gtattgtggt cggtattgat acctggtacg acgccgaaac cggtaaatat
    20461 catgtgaatc aatataccgg cgatgaaagc cgcacccgcg ttactcagtg ggaaacgctc
    20521 gactgggtac aggaagtgca aaaacgcggt gccggagaaa tcgtcctcaa catgatgaat
    20581 caggacggcg tgcgtaacgg ttacgacctc gagcagctga aaaaagtgcg tgaagtttgc
    20641 cacgtcccgc tgattgcctc cggtggcgcg ggcaccatgg aacactttct cgaagccttc
    20701 cgcgatgccg acgttgacgg cgcgctggca gcttccgtat tccacaaaca aataatcaat
    20761 attggtgaat taaaagcgta cctggcaaca cagggcgtgg agatcaggat atgttaacag
    20821 aacaacaacg tcgcgaactg gactgggaaa aaaccgacgg tctgatgccg gtgattgtgc
    20881 aacacgcggt atccggcgaa gtgctaatgc tgggctatat gaacccggaa gccttagaca
    20941 aaaccctcga aagcggcaaa gtcaccttct tctcgcgcac taaacagcga ctgtggacca
    21001 aaggcgaaac gtcgggcaat attctcaatg tggtgagtat tgccccggac tgcgacaacg
    21061 acacgttact ggtgctggca aatcccatcg gcccgacctg ccacaaaggc accagcagct
    21121 gcttcggcga caccgctcac cagtggctgt tcctttatca actggaacaa ctgctcgccg
    21181 aacgtaaatc tgccgacccg gaaacctcct acaccgccaa actgtatgcc agcggcacta
    21241 aacgcattgc gcagaaagtg ggcgaagaag gcgtggaaac cgcactggca gcaacggtac
    21301 atgaccgctt tgagctgacc aacgaggcat ctgacttgat gtatcacctg ctggtgttgt
    21361 tgcaggatca ggatctggat ttagcgacgg taattgagaa cctgcgtaaa cggcatcagt
    21421 gagttgcgtg ctgaccggat gcgatgttgt tgtcgcatcc ggagccattg cccgatgcgc
    21481 aagcttatcg tgcctacaca ttatctcaac acacgtaggc cggataagat gcgccagcat
    21541 cgcatccggc aaaaaaaacg ggcatggtgt caccaccctg cccgtttctc ttaaatgcac
    21601 aataatatta tttcgcgttg taattacgca gcgcgttacg ccccaacaca atccccacgc
    21661 caaccatgcc gcccagcagc accgccagaa tcaaggtaat tgcctttttc gggctatcgc
    21721 gacgaatagg taacgtcggt ttcattacat agcggtaagc atgaatatca agtttatcaa
    21781 cgtcaagatt gtcgatatcc agcaggtttt gacgagtctg atagtagctt gatgagaaca
    21841 ccaacggacg ggtcgcctcg tgcttaatca tcgactccag cgcttcgctc cccaaaagga
    21901 acatagtatc ctgggttacg tcctgcgtct gttgaatctg cggctttgtc acctgcgcct
    21961 gattcgcata ctgcaacgct tcctgaatct gacggatacg cagatctttc tgttcctgcg
    22021 cgaccacttc ctgggttctt aaagagtcct gcaagttttt ccgtcccaga gcaatgttgt
    22081 ccttgagatc cttttctagc tcttgattca ctttatcatc aacttgctga atgtattggg
    22141 ccaacttcat ttgtgcgccc tctgcagttt gcccaacata agaaacagtc aatggtaatt
    22201 gctggttctt aacagaaggt tcgatggtaa gtttttctgg ctcttcctga ttatccagcg
    22261 tttctgctaa tgcagagaag gcagaactga agcgaccaat taacgtctcc tgcaaatccg
    22321 atactttcgg tgtagcctga ccatagataa cattcatggc attgttatag ccagcaattt
    22381 gccccacgtc gggctgagtg ataattgctg ttgacgtcca tttctccttc gctactgcca
    22441 aataaccaat agccagggca atagccacaa tgacggaaat gatgattgtc atcttgccac
    22501 gccacaactg cactagtaaa tcaatcaaat caatctgttc cgggtcatgg ttttgcccag
    22561 aaacattatt attttctact ctcatcatta ccctaactga cggaaatttc caagtccgca
    22621 tagtttagcg ataatcctcg tgaatgctat aggataagtg ataaaaataa tgaattgttg
    22681 aggaagtaac aaaagacaat cagggcgtaa atagccctga taacaagatg ttaatcgcta
    22741 ccaaagagat cgcgggtgta taccttatct gccacatcct taagctcttc tgccatacga
    22801 ttggagataa tgacatcagc ttgttgtttg aaagtggcga gatctcgttc caggcgagag
    22861 ttgaagaatg agtcttcttt catcaccggt tcgtaaatga tcacttcaac gcctttcgcc
    22921 ttgatacgtt tcataatccc ctgaatggaa gacgcgcgga agttgtctga accgctcttc
    22981 ataatcagac gataaatacc caccacttgc ggcttgcgtg acaaaatggc atcggcaata
    23041 aaatctttac gcgtgcggtt agcatcgaca attgccgaaa tcaggttatt cggcacagac
    23101 tgataattcg ccaataactg tttggtatct ttcggcaaac aataaccacc ataaccaaac
    23161 gacggattgt tgtaatggtt gccgatacgc gggtcgagac aaacaccttc gataatctga
    23221 cgcgtattca gacccaaact ttctgcatag ctatccagtt cgttaaagta cgccacgcgc
    23281 atcgccagat aggtattagc gaaaagttta atcgcttctg cttcagtgga gtcggtaaac
    23341 agggtcggga tattttgctt aatcgtgcct tcctgtaaca acgcagcaaa acgttctgcg
    23401 cgttctgaac gctcaccgat gacaatacgt gacggatgaa ggttatcgta aagggcttta
    23461 ccctcacgga gaaattccgg ggagaatata atattttcag tacgatattt tttatgcatc
    23521 gctgcggtaa aaccaacggg aaccgttgat ttgatgacca taaccgcata agggtttatc
    23581 tcaactacgt ctttaattac tgattctaca ctggatgtat tgaaataatt agttttagga
    23641 tcatagtcgg ttggagtggc gatgatgaca taatcagcat cccggtaggc ttcattttta
    23701 tctaatgtgg cattaaagtg tattttatct gattgcaaaa actgctgaat ttccttatca
    23761 acaataggag atatccgatc attcagcata gcaacgcgtg acggtaaaat atctaatgcc
    23821 acaacctcat gattttgtgc gattagaagc ccgtttgaca agcctacata gccagtaccg
    23881 gaaatggtaa ttttcatttc atgctctcag aattaactta actgtgaatc atgatgtttt
    23941 tagcatcctg acaagagtta aaagttttaa cgctacgtcg atagctggtc aacgcaacga
    24001 aaaacaattc agaatttctt tataagaata gctgatattt attaagttaa tattaagcag
    24061 tgaaatttag tctataagat aaatggcaaa aaaagcccgg cacaatatac gccgggcctc
    24121 aatttattgt tagttaagtc agattaatcc agccattcgg tatggaacac accttcttta
    24181 tcgatgcgct tataagtatg tgcaccgaaa tagtcacgct gtgcctggat caggtttgca
    24241 ggcagaacag cggcacggta gctgtcataa taggcaaccg cagcggcgaa ggtcggaacc
    24301 gggataccgt tctgtactgc ataagcaacg acatcgcgca gcgcctgctg atagtcatcg
    24361 gcgatttgct taaagtacgg agccagcagc aggttagcga tctgcggatt ttcggcgtat
    24421 gcatcggtga ttttctgcag gaactgcgca cggatgatgc agccagcacg gaaaatcttc
    24481 gcgatttcgc cgtagttcag atcccagttg tattcttcag acgcagcacg caactgagag
    24541 aagccctgag cgtaagaaac gattttgccc agatacagcg cacgacgaac cttctcgata
    24601 aactcagctt tgtcgcctgc tcgctgcgct tgcgggccag agagaacttt agacgcggca
    24661 acgcgctgat ctttcagaga agagatataa cgtgcaaaca cagactcggt aatcagcgac
    24721 agcggttcgc cgagatccag cgcgctctgg ctggtccatt tacccgtacc tttgtttgca
    24781 gcttcatcca gaatcacatc aaccaggtag ttaccgtctt catctttttt ggtgaagata
    24841 tctttggtaa tgtcaatcag gtagctgctc agttcaccgt tattccactc ggtaaaggtc
    24901 tgcgccagtt cttcgttggt gaggttcagg ccacctttaa gcagagaata ggcttcagca
    24961 atcagctgca tatcaccgta ttcaataccg ttgtgaacca tcttcacata gtggcctgcg
    25021 ccatcggcac caatataggt aacgcatggc tccccgtctt cagccactgc ggcgattttg
    25081 gtcaggatcg gcgcaaccag ttcataggct tctttctggc caccaggcat aatggaaggt
    25141 cctttcagcg cgccttcttc accgccagaa acaccagtac cgatgaagtt aaagccttct
    25201 gcagaaagct cacgattacg acgaatagtg tcctggaaga aggtgttacc accatcaatg
    25261 atgatgtcac ctttatcgag gtacggctta agggaatcaa tagcagcatc cgtgcctgca
    25321 cctgctttca ccattaacag gatgcgacga ggcgtttcca gagattcaac aaactctttc
    25381 accgtatagt aaggaaccag ttttttgcct ggattttcgg caatcacttc ttccgttttt
    25441 tcacgggaac ggttgaaaat agagacggta taaccacggc tttcgatgtt gagcgcaagg
    25501 ttgcgcccca tcactgccat accgacgacg ccgatctgtt gctttgacat tgtttactcc
    25561 tgtcagggcg ttaaaaaatt ggtatataaa gcaaagatct ttaaataaag ataccatttc
    25621 agtagtaaaa
//
LOCUS       DACADF010000067        24884 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.067, whole genome shotgun sequence.
ACCESSION   DACADF010000067 DACADF010000000
VERSION     DACADF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.067"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            41..874
                     /gene="xapA"
                     /locus_tag="ISB30_002536"
     CDS             41..874
                     /gene="xapA"
                     /locus_tag="ISB30_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HAP1990159.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGVPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            935..2191
                     /gene="xapB"
                     /locus_tag="ISB30_002537"
     CDS             935..2191
                     /gene="xapB"
                     /locus_tag="ISB30_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HAP1990160.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            2244..2402
                     /locus_tag="ISB30_002538"
     CDS             2244..2402
                     /locus_tag="ISB30_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HAP1990161.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            2443..3327
                     /gene="xapR"
                     /locus_tag="ISB30_002539"
     CDS             2443..3327
                     /gene="xapR"
                     /locus_tag="ISB30_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HAP1990162.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(3438..3513)
                     /locus_tag="ISB30_002540"
     tRNA            complement(3438..3513)
                     /locus_tag="ISB30_002540"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3478..3480),aa:Lys,seq:ttt)
     gene            complement(3518..3593)
                     /locus_tag="ISB30_002541"
     tRNA            complement(3518..3593)
                     /locus_tag="ISB30_002541"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3558..3560),aa:Val,seq:tac)
     gene            complement(3640..3715)
                     /locus_tag="ISB30_002542"
     tRNA            complement(3640..3715)
                     /locus_tag="ISB30_002542"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3680..3682),aa:Val,seq:tac)
     gene            complement(3760..3835)
                     /locus_tag="ISB30_002543"
     tRNA            complement(3760..3835)
                     /locus_tag="ISB30_002543"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3800..3802),aa:Val,seq:tac)
     gene            4094..5509
                     /gene="gltX"
                     /locus_tag="ISB30_002544"
     CDS             4094..5509
                     /gene="gltX"
                     /locus_tag="ISB30_002544"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP1990163.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            complement(5560..5952)
                     /locus_tag="ISB30_002545"
     CDS             complement(5560..5952)
                     /locus_tag="ISB30_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAP1990164.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(5954..6298)
                     /locus_tag="ISB30_002546"
     CDS             complement(5954..6298)
                     /locus_tag="ISB30_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAP1990165.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
     gene            6534..6609
                     /locus_tag="ISB30_002547"
     tRNA            6534..6609
                     /locus_tag="ISB30_002547"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6567..6569,aa:Ala,seq:ggc)
     gene            6649..6724
                     /locus_tag="ISB30_002548"
     tRNA            6649..6724
                     /locus_tag="ISB30_002548"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6682..6684,aa:Ala,seq:ggc)
     gene            6931..9120
                     /locus_tag="ISB30_002549"
     CDS             6931..9120
                     /locus_tag="ISB30_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HAP1990166.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLTFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGHFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(9170..10372)
                     /gene="nupC"
                     /locus_tag="ISB30_002550"
     CDS             complement(9170..10372)
                     /gene="nupC"
                     /locus_tag="ISB30_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAP1990167.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            10708..11946
                     /gene="mntH"
                     /locus_tag="ISB30_002551"
     CDS             10708..11946
                     /gene="mntH"
                     /locus_tag="ISB30_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAP1990168.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(12087..12413)
                     /locus_tag="ISB30_002552"
     CDS             complement(12087..12413)
                     /locus_tag="ISB30_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAP1990169.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12528..13784)
                     /locus_tag="ISB30_002553"
     CDS             complement(12528..13784)
                     /locus_tag="ISB30_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAP1990170.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            13988..14953
                     /gene="glk"
                     /locus_tag="ISB30_002554"
     CDS             13988..14953
                     /gene="glk"
                     /locus_tag="ISB30_002554"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAP1990171.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            15172..15498
                     /locus_tag="ISB30_002555"
     CDS             15172..15498
                     /locus_tag="ISB30_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAP1990172.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            15520..16767
                     /locus_tag="ISB30_002556"
     CDS             15520..16767
                     /locus_tag="ISB30_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP1990173.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            16782..17867
                     /gene="ypdF"
                     /locus_tag="ISB30_002557"
     CDS             16782..17867
                     /gene="ypdF"
                     /locus_tag="ISB30_002557"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP1990174.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISVIRPGVRCQQVDDAARRV
                     ITEAGYGKFFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAVPKTVLLTGEA"
     gene            17867..18904
                     /gene="ypdE"
                     /locus_tag="ISB30_002558"
     CDS             17867..18904
                     /gene="ypdE"
                     /locus_tag="ISB30_002558"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP1990175.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILREEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDSDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            18929..21424
                     /gene="ptsP"
                     /locus_tag="ISB30_002559"
     CDS             18929..21424
                     /gene="ptsP"
                     /locus_tag="ISB30_002559"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP1990176.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKLTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(21427..22284)
                     /locus_tag="ISB30_002560"
     CDS             complement(21427..22284)
                     /locus_tag="ISB30_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1990177.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPTSLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVLLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWIQDNYAQLLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(22297..23031)
                     /locus_tag="ISB30_002561"
     CDS             complement(22297..23031)
                     /locus_tag="ISB30_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP1990178.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSCSKVKEFRQLMHL"
     gene            complement(23046..24743)
                     /locus_tag="ISB30_002562"
     CDS             complement(23046..24743)
                     /locus_tag="ISB30_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP1990179.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         5867 a         6064 c         6644 g         6309 t
ORIGIN      
        1 aaaaaggcgt tagattcccc cctacaaaaa aaggatatat atgtatcagg ctcagttttc
       61 tcataaccca ctgtattgcg tagatattat caagacttat aaacctgatt tcacgccacg
      121 agtggccttt attttaggtt ccgggctggg cgcgctggcc gatcagattg agaacgcggt
      181 cgcaatttcc tacgaaaagc tgcctgggtt cccggtaagt accgtacacg gtcatgcggg
      241 tgagctggtg ctggggtatc tccagggggt gccagtggtg tgcatgaaag gtcgcggaca
      301 tttctacgaa ggtcgtggga tgaccatcat gacggatgca atccgtacct ttaagttgct
      361 gggctgcgag ttgctgttct gcaccaatgc ggctggctca ctgcgccctg aagtgggggc
      421 cggcagtctg gtcgcattga aagatcacat caacaccatg ccgggaacgc cgatggtggg
      481 tcttaatgat gaacgttttg gtgagcgctt cttctcgctg gcgaatgcct acgatgcgga
      541 ataccgcgca ctgttacaaa aagtggcgaa agaagagggg gtccctctga cggagggcgt
      601 gttcgtctcg tatccggggc cgaatttcga gactgcggcg gaaattcgca tgatgcaaat
      661 tattggtggg gatgttgttg gtatgtctgt ggtgcctgag gttatttcag ctcgccattg
      721 cgaacttaaa gtcgttgcgg tctctgcgat taccaacatg gcggaaggtc tgagtgacgt
      781 gaagctttct catgcccaaa cgctggcagc agcggaactc tcaaagcaaa actttattaa
      841 tcttatttgc ggctttctgc gcaaaattgc ctgagtaaaa caacaataaa cggccttact
      901 gagtgaggcc atatgatgcg acaaggaaac gattatgagc atcgctttgc gcttaaaggt
      961 aatgtccttt ttgcaatatt ttatctgggg gagctggctg gttaccctcg gttcttacat
     1021 gattaatacc cttcatttca ccggcgctaa tgttggcatg gtttacagtt ccaaagggat
     1081 cgccgcgatt attatgcctg gtataatggg gatcatcgca gacaaatggc tgcgtgcaga
     1141 acgtgcatac atgctgtgtc acctggtgtg tgcgggcgta cttttttatg cggcatccgt
     1201 aactgatccg gatatgatgt tttgggtgat gttagtcaat gcgatggcgt ttatgccgac
     1261 tattgcgtta tcgaacagcg tctcttattc ctgtcttgcc caggcagggc ttgacccggt
     1321 gaccgctttc ccgcccattc gcgtttttgg tacggtgggg ttcattgtcg ccatgtgggc
     1381 agtaagcttg ctgcatctgg aattgagtag tctgcaactg tatatcgcgt ccggtgcgtc
     1441 attgctgctg tcggcttatg cgctgacttt gccgaagatt ccggttgcgg agaaaaaagc
     1501 gactacatcg cttgccagca agctgggtct ggatgccttc gtgctgttta aaaatccacg
     1561 catggctatc tttttcctct tcgccatgat gctgggtgcg gtgctgcaaa ttaccaacgt
     1621 ttttggcaac ccgttcctgc atgatttcgc ccgtaatcct gagtttgctg atagcttcgt
     1681 ggtgaaatac ccgtccattt tactgtcagt ttcacagatg gctgaagtcg gtttcattct
     1741 gacgataccg ttctttttga agcgtttcgg tattaaaacc gtcatgctga tgagtatggt
     1801 ggcgtggacg ctgcgctttg gcttctttgc ctatggcgat ccatcaccaa ccgggtttat
     1861 tttgctgttg ctgtcgatga ttgtttacgg ttgtgcattc gatttcttca atatttctgg
     1921 ttcggtcttt gtcgaacagg aagttgattc cagcattcgt gccagcgccc agggcctttt
     1981 tatgacgatg gtaaatggcg tcggcgcatg ggttggctcg attctgagcg gtatggcagt
     2041 ggattacttc tcggtggatg gcgtaaaaga ttggcatacc atttggctga tgtttgcagg
     2101 gtatgctctt ttcctcgcag tgatattttt ctttgggttt aaatataacc atgaccctga
     2161 aaagataacg catcgttcag ttacacatta aaaggattcg cagctctgtt ccttggggct
     2221 gcttttatga taaaggttaa ttagtgaaag atattttatt ttcttctggc gttggttttg
     2281 gcattggtgc tttgtttaca atcgtgcgat tacctattcc tgttccaaat gtattgccgg
     2341 gtatattatc catcgtgttt atgtatgtcg gatatctggt ggtgaaatac tttatgccat
     2401 gataatttaa tacgatgtat ttattatatg gagcacttaa ttatggaacg cgtatataga
     2461 acagatctta agttgctccg ttattttctt gccgtagcgg aagagttgca ttttggtcgc
     2521 gcagcagcgc gtttaaatat gtctcagcct ccgctcagta ttcatattaa agagctggaa
     2581 aatcaactcg gcacgcagct ttttattcgc cattcgcgca gcgtcgtact gacacacgcg
     2641 ggcaaaatct tgatggaaga atcgcgtcga ttgctggtga atgcaaataa tgtattggct
     2701 cgggttgaac aaataggtcg gggagaagca gggcggattg aactcggggt tgtgggaacg
     2761 gcaatgtggg gacggatgcg cccggttatg cggcgattcc ttagagaaaa tcctaacgtt
     2821 gaagttctgt ttcgcgaaaa gatgcccgcg atgcaaatgg ccttgctgga acgccgcgaa
     2881 cttgatgccg ggatctggcg aatggcgaca gaaccaccga ctggttttac cagcttacgg
     2941 ttgcatgaat cggcgtttct ggtggcgatg cctgaagagc atcatctctc atcattttcc
     3001 accgtcccgc tggaagcgct acgtgacgag tatttcgtta caatgccgcc cgtgtacact
     3061 gactgggatt ttttgcagcg agtttgccag caggtgggat tttcaccggt tgttattcgc
     3121 gaagttaatg aaccgcaaac ggtactcgcc atggtcagta tgggtattgg tatcacattg
     3181 atagcggaca gctacgcgca gatgaactgg cccggtgtca tattccgtcc gctcaaagaa
     3241 cgcatcccgg cagacttata tattgtttat gaaacgcagc aggtgacgcc cgcgctggtt
     3301 aaactgctgg cggcgttggc gcagtagatg agaggcacgg gaactgccgg acatagttgg
     3361 tctgataagc gaagcgcatc aggcaatttc gcaaattgca gatagcaaaa aaacgccttt
     3421 agggcgcttt tttacattgg tgggtcgtgc aggattcgaa cctgcgacca attgattaaa
     3481 agtcaactgc tctaccaact gagctaacga cccgaagtgg tgggtgatga cgggatcgaa
     3541 ccgccgaccc cctccttgta agggaggtgc tctcccagct gagctaatca cccacttcgg
     3601 tacttcacat tgttacaaga aatctagctg gcgagaaagt ggtgggtgat gacgggatcg
     3661 aaccgccgac cccctccttg taagggaggt gctctcccag ctgagctaat cacccacttc
     3721 tcaatttctt gctacacggc ggagactaca taaagtagtt ggtgggtgat gacgggatcg
     3781 aaccgccgac cccctccttg taagggaggt gctctcccag ctgagctaat cacccccgct
     3841 gtgtggagtc gcattatagg gagagttcaa aatgagtcaa cgcattttct aaagaaattg
     3901 ttcgttcgtc gtaaatttaa gcaagatgat cgcaaaacag accgtgttgc gcaatttgtc
     3961 aacgaaaaca ataatgcgta aggttgcaac ccgaactaca ttgaggaatc aggcgggagt
     4021 gatagaatat cgcccactca atttttccag gatttgccgg ttgtcggcat ctttctaaac
     4081 gtaaggccat ttcatgaaaa tcaaaactcg cttcgcgcca agcccaacag gctatctgca
     4141 cgttggcggc gcgcgtactg ctctttactc ctggcttttt gcacgtaacc acggcggtga
     4201 gttcgtgctg cgtattgaag acaccgatct tgagcgttcc acgccggaag ctatcgaagc
     4261 cattatggat ggcatgaact ggctgagcct ggagtgggat gaaggtccgt actaccagac
     4321 caaacgtttt gatcgctaca acgcggtgat cgatcagatg ctggaagagg gcacagctta
     4381 taaatgctat tgctctaaag agcgcctgga agcgctgcgc gaagagcaaa tggcgaaagg
     4441 tgagaagccg cgttatgacg gtcgctgccg ccacagtcat gagcatcacg ctgatgatga
     4501 gccgtgtgtc gtacgttttg ctaacccgca ggaaggttct gttgtttttg acgatcagat
     4561 ccgtggtccg atcgagttca gcaaccagga gctggatgat ctgattatcc gccgtaccga
     4621 tggttcccca acctataact tctgtgtggt tgtcgatgac tgggatatgg aaatcaccca
     4681 cgttatccgt ggcgaagacc atatcaacaa cacaccgcgc cagattaaca ttcttaaggc
     4741 tctgaaagcg ccggtaccgg tttatgcgca cgtttctatg atcaacggcg atgacggtaa
     4801 aaaactgtcc aaacgtcacg gggcggtcag cgtaatgcag tatcgtgatg atggttatct
     4861 gccagaagca ctgctgaact atctggtgcg tctgggctgg tcccacggcg atcaggaaat
     4921 cttcactcgt gaagagatga ttaaatactt cactctgaat gccgtcagca aatctgccag
     4981 tgcgttcaac accgacaagc tgctgtggct gaaccatcac tacattaacg cgttgccgcc
     5041 ggagtatgtt gctactcact tacagtggca cattgagcag gaaaatatcg atacccgtaa
     5101 cggcccgcag ctggctgatc tggtgaaact gttgggcgaa cgctgcaaga cgctgaaaga
     5161 gatggcacag agctgccgtt atttctacga agattttgct gagttcgatg ccgacgccgc
     5221 gaaaaaacat ctgcgtccgg tagcgcgcca gccgctggaa gtggttcgtg acaaactgac
     5281 tgcgattact gactggaccg ctgaaaacgt tcatcacgct attcaggcga cggcggatga
     5341 gctggaagtg ggtatgggta aagttggtat gccgctgcgt gtcgccgtaa ccggtgcggg
     5401 gcagtctccg gcgctggatg ttaccgttca cgcaatcggt aagacccgca gtatcgagcg
     5461 tatcaacaaa gcgctggctt ttattgctga acgcgaaaat cagcagtaat tagcgcataa
     5521 aagataaacg gcaggagaga tacccctgcc gttttttatt tatgctgatt catctatgtc
     5581 gagacgagca agaaaattac gaattccttc gcgtgacaaa aattgcgcaa ctttttcttg
     5641 ctcactgtga gtcatgttgt ctattgcgct gatgatttgc cgataagcag gagtgcgaat
     5701 acccggtgcg tattcggcaa ggggttcttc ggcttccaga agcatcggtg tgttatggaa
     5761 ggctggcgtg ttctgaataa actcgcaaac ttgcgtatcg accagaataa gtctggcacg
     5821 accgcctttc acgccgggaa atttttccgt tttccagcct ttttctctga tccaacggtt
     5881 aacagtttgt ttagccacac cgaggcattc agccagttct tcggtggtca ttttattgcg
     5941 taatcttttc atattatttg ctatcgcgaa tccctaagcg ttgtaacaat ccgataatcc
     6001 cttcccgcag aagcaaggat gtaaactgtt tttgttcaac tggcgtcatc tctttcgcca
     6061 gtgtcaccag caacacttca agcggcgcat caccagaaag ggctggcgct tctccctgac
     6121 cttctggacg ttcagcattg cgaatatatt cacgaacctg ttcattgacg tgaaccagtc
     6181 gagccttgcc accctggacg cctggttttg gtgacgttgt ccagccttcc ttgcgtaccc
     6241 atttattaat ggtctggcgg ctatagccgg tcagtctggc aagttcatct ggcgtcatcc
     6301 tctccttgaa cataacaatt ccctgaatgt atgtgggtct attagtagtt cattttataa
     6361 caccacttta caggaaaccg tgacatgaat aactactgaa tgcgagcggt tttccaaaat
     6421 tgttcatttg cctgattttt cagcgattga aactacacgt ggcaatttgc cgttgacaca
     6481 ttcgggcgga attcatatga tgccgcccgt caactcgaca agctttacgt gatggggcta
     6541 tagctcagct gggagagcgc ttgcatggca tgcaagaggt cagcggttcg atcccgctta
     6601 gctccaccaa attttgcacc cagcaaattt ggtacgtaaa cgactcgtgg ggctatagct
     6661 cagctgggag agcgcttgca tggcatgcaa gaggtcagcg gttcgatccc gcttagctcc
     6721 accaaatttc aaaccctcgc tgcaaagcgg gggttttttg tctctgcctt ttgccgcttt
     6781 tgtaatacag tctacatcct ggcagtgccg tgtggtgaat gcatcactga ctgcaaaaac
     6841 agcaacaggc tggcccctgt ttgcttcgcg atgcgaataa acttattatt tgtgtgcctg
     6901 aaaaccccga tcagtgagag tagtgtactc atgtttgtgg agcataacct gataaaaaat
     6961 atcaagatat tcacactagc gtttacgctc accgtggtac ttattcagct atcccgttta
     7021 atttcgccac ttgctgttat ccattccaat tatatctttc tggcgtggat gccactgtgc
     7081 gtaatgctgt caatcttgtt tatctttggc tggcgtggtg tcgttcccat tttatgtggg
     7141 atgttttgca ccaatctgtg gaaccttcat ctcacttttt tacagaccgc cgtcatgatc
     7201 ggcagccagg cgtttgcagt cctgtgtgcc tgtgcaatat tacgctggca attggggacg
     7261 cgttggcgtt atggattgac cagccgatac gtctggcaac gtctgttctg gcttggtttg
     7321 gtggcgccga ttggcatcaa atgcagcatg tatcttgtgg gaaatttctt tgattttccg
     7381 ctaaagatat ctaccttttt cggcgatgcg gatgccattt tcacggtcgt tgatttgcta
     7441 agccttttca ccgcagtgct gatttacaac atgctttttt actatctcac ccgcatgatt
     7501 gtaagtcccc actttgcaca gatattgtgg cgtagggata tcgctccgtc gttgagcaaa
     7561 gagaaacgcg catttacctt aagctggctg gcagctctta gcgtgctgct acttctgatg
     7621 tgcacaccgt atgaaaatga ctttattgcc ggttacttgg tacctgtttt ctttatcatc
     7681 tttaccctcg gtgtcggtaa gcttcgctat ccgtttttaa atctcacctg ggctgtttca
     7741 acgctttgcc tgctgaatta caaccagaac tttttgcaag gggtactaac cgaatattcg
     7801 ctggcattta ttctcgcagt actgatttcc tttagtgttt gtctgctcta tatggtgcgt
     7861 atttatcatc gtagtgaatg gcttaaccgc cgctggcatt tgcaggcgct gaccgatccg
     7921 ttaacgctcc tacccaactt tcgtgcgttg gaacaagcac cggagcaaga ggcgggcaag
     7981 agtttttgct gcctgcgcat tgataatctt gagtttatga gtcgtcatta cggcttaatg
     8041 atgcgcgttc actgtatccg ctcaatttac cgtacgttgc tgccgttgat gcaggaaaac
     8101 gaaaagttgt atcaattgcc gggtagtgaa ctgctgttag tgctgagcgg gccggaaacg
     8161 gaagggcgac tccagcatat ggttaacatc ctgaatagtc ggcaaattca ctggaacaat
     8221 accgggctgg atatgggcta tggtgctgcc tgggggcatt ttgatggaaa tcaggaaacc
     8281 ctgcaaccct tgttggggca gttaagctgg ctggcagagc aatcctgcgc acatcatcat
     8341 gtgctggcgc tggatagcag agaggagatg gtttccgggc aaaccactaa acaggtgcta
     8401 ttgctgaata ccattcgcac ggcgttagat cagggtgatt tgctgctcta cgcccagcca
     8461 attcgcaaca aagagggtga aggttatgat gagatcctcg cgcgactgaa gtatgacggc
     8521 ggcattatga ccccggataa gtttctgccc cttattgctc agtttaacct tagcgcgcgt
     8581 tttgatttgc aagtgctgga atccttgttg aagtggctgg caacacaccc ttgcgacaaa
     8641 aaaggtccac gattttcagt caatttaatg ccgctcacgc tgctgcaaaa gaatattgcc
     8701 gggcggatta ttcgtctgtt taagcgttat tacatctccc cgcaggcggt cattcttgag
     8761 atcaccgagg agcaggcgtt ttctaacgca gaaagcagca tgtacaacat cgagcagctg
     8821 cataagtttg gtttccggat tgcgattgat gactttggca ccggatatgc caactacgaa
     8881 cggttaaagc gtttgcaggc tgatatcatc aaaattgatg gcgtctttgt gaaagatatc
     8941 gtcacgaaca cgctggatgc gatgattgtg agatcaatta ccgatctggc gaaagcgaag
     9001 tcattgagtg tggtcgcgga gtttgtcgag acgcaacagc agcaggcgct attgcataag
     9061 ctcggggtgc aatatctgca agggtatttg attggtcgcc cgcagccatt agccgattaa
     9121 caggcgtaaa aaagccgggg aattatcccc ggctttttta tgtatggtct tacagcacca
     9181 gtgctgcgat tgacgcagac agcacactca ccagggtaga gccgtaaacc agtttcagac
     9241 cgaagcgaga aaccacgtta ccttgctctt cattcaggcc tttaaccgca cccgcgataa
     9301 tcccgattga agagaagtta gcgaaggaaa ccaggaacac agagatgatg ccttccgcac
     9361 gcggagagag cgtggaagca attttctgca gatccatcat cgcaacgaat tcgttggaaa
     9421 ccagtttggt cgccatgata ctgcccactt gcagtgcttc actggaagga acacccatca
     9481 cccatgcaat cggatagaag atgtaaccca ggatgccctg gaaggagatg ctgtagccaa
     9541 accagccagt cacggtggca aacagtgcgt tcagcgcggc gatcagggcg ataaagccga
     9601 tcagcatcgc cgcaacgata atggcaacct tgaaacctgc cagaatgtat tcacccagca
     9661 tttcgaagaa gctctgacct tcgtgcaggt tggacatctg aatattttct tcactggcat
     9721 caacacggta agggttgatc agcgacagca cgataaaggt gctgaacatg ttcagtacca
     9781 gcgccgcaac gacgtatttc ggctccagca tggtcatgta tgcaccaacg atggacatcg
     9841 acacggtgga catcgccgtt gctgccatgg tgtacatacg attgcgggag attttgccga
     9901 ggatatcttt ataggcaata aagttttcag actgacccag aatcagggag ctgacggcgt
     9961 taaaggattc cagtttgccc atgccgttga ctttggagag caggaaacca attgcgcgga
    10021 taatcaccgg caacacgcga atgtgctgga gaataccgat cagtgcagag ataaagacga
    10081 ttgggcacag cactttcagg aagaagaatg ccaggccttg atcattcatg ctaccaaaga
    10141 cgaagttagt cccttcgttg gcaaaaccga gcagtttttc gaacatttcg gagaagcctt
    10201 tcacgaagcc cagaccaacg tcggagttca ggaagaacca cgccagtaac acttcgataa
    10261 caagcagttg aataacataa cggatacgaa tttttttgcg gtcgctgctt accagcagtg
    10321 cgagaatcgc aacaacggca agtgccagta caaaatgaag gacgcggtcc atatttgctc
    10381 caaatatgag gcaggttaaa tttccgtgca cattctatgt aacatggtta aggaaaacga
    10441 gatctgacac acactataat gacgtcctgt gacaagcttc aaaaatagtg atctgtcata
    10501 catttctgct atgttgtgta tggaagcgga aggttatgta aatgtgctaa tattaaacat
    10561 aaccataaca ttcgagtcat ctatcttttc gtagtcataa tcctggtcta tcagagaaat
    10621 catcacaacc catttaaatg aatttgataa tcattctcat ttaacgtagc atgaaacata
    10681 gcaaaggcta tgttttagag gcacaagatg acgaactatc gcgttgagag tagcagcgga
    10741 cgggcggcgc gcaagatgag gctcgcatta atgggacctg cgttcattgc ggcgattggt
    10801 tatatcgatc ccggtaactt tgcgaccaat attcaggcgg gtgccagctt cggctatcag
    10861 ctactgtggg ttgtcgtttg ggctaacctg atggcgatgc tgattcaaat cctctctgcc
    10921 aaactaggga ttgccaccgg taaaaatctg gcggagcaga ttcgcgatca ctatccgcgt
    10981 cccgtagtgt ggttctattg ggttcaggca gaaattattg cgatggcaac cgacctggcg
    11041 gaatttattg gtgcggcgat cggttttaaa ctcattcttg gtgtttcgtt gttgcagggc
    11101 gcggtgctga cggggatcgc gactttcctt attttaatgc tgcaacgtcg cgggcaaaaa
    11161 ccgctggaga aagtgattgg cgggttactg ttgtttgttg ccgctgctta cattgtcgag
    11221 ttgattttct cccagcctaa cctggcgcag ctgggtaaag gaatggtgat cccgagttta
    11281 cctacttcgg aggcggtctt cctggccgcg ggcgtgttag gggcgacgat tatgccgcat
    11341 gtgatttatt tgcactcttc gctcactcag catttacatg gcggttcgcg tcaacaacgt
    11401 tattccgcca ccaaatggga tgtggctatc gccatgacga ttgccggttt tgttaatctg
    11461 gcgatgatgg ctacagctgc ggcggcgttc cacttttctg gtcatactgg tgttgccgat
    11521 cttgatgagg cttatctgac gctgcaaccg ctgttaagcc atgctgcggc aacggtcttt
    11581 ggattaagcc tggttgctgc cgggttgtcc tcaacggtgg tggggacgct ggcggggcag
    11641 gtagtgatgc agggcttcat tcgctttcat atcccgctgt gggtgcgtcg tactgtcacc
    11701 atgctgccgt catttattgt cattctgatg ggattagatc cgacacggat tctggttatg
    11761 agtcaggtgc tgttaagttt tggtatcgcc ctggcgctgg ttccactgtt gattttcacc
    11821 agtgacagca agttgatggg cgatctggtg aacagcaaac gcgtaaaaca gacaggctgg
    11881 gtgattgtgg tgctggtagt ggcgctgaat atctggttac tggtaggaac ggcactggga
    11941 ttgtagttga atgagcgtcg catttggcac cattggcgga tgcggcgtaa atgctttatc
    12001 cgccctacgc gttaaagagc tggtttgtag gcctgataag acgcgctagc gtcgcatcag
    12061 gcattgtgct ccattggcga atgtgtttaa tggcggtgtc catgtccgcg gccttttcca
    12121 cgatgatcgt cacgatcgcg ccagccttca cgatagccac gctcatacgc tttacgctta
    12181 tcccagccac gatggtagcc attatcatga cgccaccagc ggtttttacg ccattcataa
    12241 ttgcggtgcc agtagtcacg gtcgcgccag tgaccaccgt cccagtaatt accgtaatga
    12301 tcgcgatcgc caatttgtaa tttgattgat ggcagtaggg tgatttcacc tgcgtttgcc
    12361 gcaagcgggg taaatgccat cagggcggcc gccagaaaca gtgacctgaa cattgttatt
    12421 ctccttcacg atcgaagccg tcagcggcct gttaacgcaa tattacgggg aggtaaagcg
    12481 ccgtatcatc gccataactc ttaaatcata aaggagagca ttttttgcta aacgttatcg
    12541 tggggtggct gttgcttcgg acgattgacc atcatcatcg gcttacctgc taataacagc
    12601 catgccggga gcatgacgat acagagcagc ggtagcaatg tggtattggg tacaacgact
    12661 gccgccataa aaagacttaa ccagccatca cgtgttacca ccagcacgat gccgagaata
    12721 gcgcaggaaa cggttattgc cgctggaacg gcgggtacgt gctcatgcag catcaaccct
    12781 aatgccacgc cgacaaacac tgccgggaag attcgcccac cgcgaaagcc actggcggca
    12841 gcaacgacca gggcggcaag ttttatcacc gccagcaaaa agtaatcgct ggtgctgaaa
    12901 gcctgatttg ccaccatctg ctgcatctca tccagccctt taaacagcga aaccggtcca
    12961 ccaataaccc ccagaatacc gagaataaat ccgccaatac ccagcacgaa caccggattt
    13021 ttcatttgat gcatcatcgc gtgcaaccgt ggtaagcacc atacggcgac catccctgct
    13081 gcgatggcaa tcgccgcgac aattgcaccg ctgagaatat cggtcatttc catctgtccg
    13141 taatgcgcaa tgggcagtga aaaatgagga tggaaaaata atccggtagt aagtgcacca
    13201 gctgctgccg ccattaacgg cgcaaagaga cgatcccata gcggaacttc attactgcca
    13261 tttaaggttt gcgaaaatat caatgccgcc gcaacaggtg tgccaaacag cgcgccgatg
    13321 gttccggcag aggctaaaat agtccactcc attcggttga cgcgcggtaa cagacgagcg
    13381 ccaatggcga cggcaagggc gatattgacg gtcatgatcg gatgttccgg ccccaggctg
    13441 acgccgccag caagaccgag aattaatgct acgataagtc ctggtagcgc agagggcgga
    13501 accggtgcgc cgatcagcgg ttcacaggcg gggtctggtc cggcatgacc ctggctgaaa
    13561 cggataacca accccaccgc aatacccgtt agcgttaata caccgatgat ccagagaggt
    13621 gaatcctggg ctatccccag agttcccggc agtcgttgcc agagcaaatt ctgtaatacc
    13681 gaggcgattt tcatcaccac aatcagaata agactggacg caatcccaat tgccacggcg
    13741 gggagcgata ataacaacat ggttctggct cgcggatgga gcatgttcta ttccttatgc
    13801 ggggtcagat acttagtttg cccagcttgc aaaaaggcat cgctgcaatt ggtgctgaaa
    13861 cgataaagta attgtgtgac ccagatcgat atttacaggg agcctgcctt tccggcgttg
    13921 ttgttatgcc cccaggtatt tacagtgtga gaaagaatta ttttgacttt agcggagcag
    13981 ttgaagaatg acaaagtatg cattagtcgg tgatgtgggc ggcaccaacg cacgtcttgc
    14041 tctgtgtgat attgccagtg gtgaaatctc gcaggctaag acctattcag ggcttgatta
    14101 ccccagcctc gaagcggtca ttcgcgttta tcttgaagaa cataaggtcg aggtgaaaga
    14161 cggctgtatt gccatcgctt gcccaattac cggtgactgg gtggcaatga ccaaccatac
    14221 ctgggcgttc tcaattgccg aaatgaaaaa gaatctcggt tttagccatc tggaaattat
    14281 taacgatttt actgctgtgt cgatggcgat cccgatgctg aaaaaagagc atctgattca
    14341 gtttggtggc gcagaaccgg tagaaggtaa gcctattgcg gtttacggtg ccggaacagg
    14401 gcttggggtt gcgcatctgg tccatgtcga taagcgttgg gtaagcttgc caggagaagg
    14461 cggtcacgtt gattttgcgc cgaatagtga agaagagggc attatcctcg aaatattgcg
    14521 tgcggaaatt ggtcatgttt cggcggaacg cgtgctttct ggccctgggc tggtgaattt
    14581 gtatcgcgca attgttaaag ccgacaaccg cctgccagaa aatctcaagc caaaagatat
    14641 taccgaacgc gcgctggctg acagctgcac cgattgccgc cgcgcgttgt cgctgttttg
    14701 cgtcattatg ggccgttttg gcggcaatct ggcgctcaat ctcgggacat ttggcggcgt
    14761 gtttattgcg ggtggtatcg tgccgcgctt tcttgagttc ttcaaagcct ctggtttccg
    14821 cgccgcattt gaagataaag ggcgctttaa ggaatatgtc catgatattc cggtatatct
    14881 catcgtccat gacaatccgg gtcttctcgg ttccggtgca catttacgcc agaccttagg
    14941 tcacattctc taaatccttc ctgttatatc gggaggtaac tctcccgata atcttttaaa
    15001 tcatacagtt aattaaattt ttctttgtgt cccctcacaa ggccgacctg cgtcacactt
    15061 ccgtacagcg ggattaattc tccagtaaat gcattatttg tctggtaacg gcgatttgtt
    15121 ttgcacgttc ataatttcac tcgtcaacta tcactgaacg aggaattaac gatgagtaag
    15181 aaactgattg ccttatgtgc ctgcccaatg ggcctggctc acacttttat ggccgctcag
    15241 gcgctggaag aagcggcggt agaagccggt tatgaagtga aaattgaaac ccagggcgcg
    15301 gacggtatcc agaatcgcct gacggcgcag gatatcgccg aagcgaccat catcatccac
    15361 tccgtggcag ttaccccgga agataacgag cgtttcgaat cacgcgacgt ttatgaaatc
    15421 actttgcagg acgcaattaa aaacgctgcg ggaatcatca aagaaatcga agagatgatt
    15481 gcctctgaac aacagtaata ttcgacaggg ataattgtta tggccattaa aaaacgcagt
    15541 gcaaccgttg tgcatggcgc atccggtgcg gcagcggcag ttaagaatcc gcaggcctct
    15601 aaatccagct tctggggtga actcccgcag catgtgatgt cagggatttc acgcatggtg
    15661 ccgaccttaa ttatgggcgg tgtgatcctc gctttcagtc agctgattgc ttatagctgg
    15721 cttaaaattc ctgctgatat cggcatcatg gatgctctta atagcgggaa attctccggt
    15781 ttcgacctct ccttacttaa atttgcctgg ctgtcgcagt ccttcggcgg cgtgttgttt
    15841 ggttttgcaa ttccgatgtt tgctgctttt gtggcgaact ctatcggtgg caaactggcg
    15901 ttcccggctg gttttatcgg cggcttgatg tctacccagc cgacgcaact gctgaacttc
    15961 gatcccagca ctatgcaatg ggcgacctct tcgccggtgc cgtccacctt cattggtgcg
    16021 ctgattattt ctatcgttgc cggttacctt gtgaaatgga tgaaccagaa aatccagtta
    16081 ccggatttcc tgttagcgtt caaaaccaca tttttgctac cgattctttc cgccattttt
    16141 gtcatgctgg cgatgtacta cgtcatcacc ccctttggtg gctggatcaa cggcggtatc
    16201 cgtaccgtgc tgaccgctgc aggtgagaag ggcgcactga tgtacgcaat ggggatcgcc
    16261 gcggcgacag caatcgacct tggtggcccg atcaacaaag cggcaggttt cgttgccttc
    16321 agctttacca ctgaccacgt attaccggtc actgcacgtt ctatcgctat cgttattccg
    16381 ccgattggtc tgggtctggc gaccattatt gaccgtcgtt taaccggcaa acgcttgttc
    16441 aacgcccagc tttatccgca gggtaaaacc gccatgttcc tggcgtttat ggggatcagt
    16501 gagggtgcga ttccgtttgc actggaaagc cctatcaccg ccattccgtc gtatatggtc
    16561 ggcgcgattg tcggctcaac cgccgctgtc tggctgggcg cagtgcaatg gttcccggag
    16621 tccgctatct gggcatggcc gctggtcact aacctcggcg tctacatggc ggggatcgcg
    16681 ctgggggcag tcataactgc gctgatggtg gtgttcctgc gtctgatgat gttccgtaaa
    16741 ggcaaattgt taatcgatag cctgtaagaa ggacaatcct gatgacatta ctcgcttcgc
    16801 tgcgcgactg gcttaaggcg caacaactgg atgcggtgct tctctcctca cggcagaaca
    16861 aacagccgca tctggggatc tccaccggat caggttatgt ggtgattagc cgtgaaagtg
    16921 cgcacattct ggtggattcg cgctattacg cggatgtaga agcccgcacg caaggttacc
    16981 agctgcattt gctcgacgcg atgcacacgc ttgccaccat cgtcagacaa atcattgccg
    17041 atgagcagtt gcaaacgctc ggttttgaag gccagcaggt gagttgggaa accgcgcacc
    17101 gctggcagtc cgaactcaat gcgaaactgg tgagcgccac gccggatgtg ctgcggcaaa
    17161 tcaaaacgcc agaagaggtg gagaaaatcc gccttgcctg cgggattgcc gatcgtggcg
    17221 cagagcatat tcgccgcttt attcaggcgg gaatgagcga atgcgagata gccgctgaac
    17281 tggagtggtt tatgcgccag cagggcgcag aaaaagcctc ttttgacacc attgtcgcca
    17341 gcggctggcg tggggcgctg ccgcacggca aagccagcga caagattgtt gcagcgggcg
    17401 agtttgtcac tctcgatttc ggtgcgctgt atcagggcta ctgctctgat atgacgcgca
    17461 ccttgctggt gaatggcgaa ggggtgagcg ccgaatctca tccgctgttt aacgtctatc
    17521 agattgtcct gcaggcacag ctcgcggcaa tctctgtaat tcgccccggc gtgcgctgcc
    17581 agcaggttga cgatgccgcg cgccgcgtga ttaccgaggc cggttatggc aagtttttcg
    17641 gccataacac cggtcatgct atcggtattg aagtccatga agaaccgcgt ttttcaccgc
    17701 gggacaccac gacgctacag ccaggtatgt tactgaccgt ggagccaggg atttatttgc
    17761 cagggcaagg gggcgtgcgc atcgaagatg tcgtgctggt caccccgcaa ggcgcagaag
    17821 tgctctacgc cgtgccgaaa acagtgttgc tcacgggaga ggcataatgg atttatcgct
    17881 cttaaaagcg ttgagcgagg cagatgcgat cgcctcctca gaacaggaag tacggcagat
    17941 cctgcgggaa gaagcggatc gcctgcaaaa agaagtgcga tttgacggcc tgggatcggt
    18001 gctgatccga ctcaatgagt cgacaggtcc gaaggtgatg atctgtgcgc atatggacga
    18061 agtgggattt atggtgcgca gcatctcccg cgaaggggcg atcgatgtgt tgccggttgg
    18121 caatgtacgc atggctgccc gccagcttca gtcggtgcgt atcactaccc gtgaagagtg
    18181 caaaattcca ggcctgcttg acagcgaccg gcaggggaat gacgtcagcg ccatgcgcgt
    18241 ggacattggc gcgcgctcct gtgacgaagt gatgcaggca ggaattcgtc caggcgatcg
    18301 cgtcaccttc gataccactt ttcaggttct ccctcaccag cgggtgatgg ggaaagcctt
    18361 tgatgaccgc ctcggttgct atctgctggt gacgttactg cgtgagctgc acgacgccga
    18421 actgcctgcg gaagtgtggc tggtggcaag ttccagcgaa gaggtgggat tacgcggcgg
    18481 gcaaactgcc acccgcgcgg tgtcgccgga tgtcgccatt gtgcttgata ctgcctgctg
    18541 ggcgaaaaac tttgattatg gcgcagctaa ccatcgccag attggtaacg ggccgatgct
    18601 ggtgttaagc gacaagtcac tgattgcgcc gccaaaactt accgcctgga tcgaaaccgt
    18661 ggcggcagaa attggcgtac cgttgcagtc agatatgttc agcaatggcg gcacggacgg
    18721 tggggcggtg cacttaaccg gcactggcgt acccacagtg gtgatggggc ctgcaacccg
    18781 ccacggacat tgcgccgcat cgattgctga ctgccgtgac attttgcaga tggagcaact
    18841 tttatctgcc cttattcaac gtcttacgcg tgagacggtt gttcaactga cggatttcag
    18901 atgatctcct gattaacccg gagcggttat gttaacgatt caatttctct gtcctttgcc
    18961 taacggtcta catgctcgtc cggcgtggga acttaaagag cagtgcagcc agtggcaaag
    19021 cgaaatcact tttattaacc atcgccagaa cgcaaaggcc gatgcgaaaa gctcgctggc
    19081 gctgattggc actggcactt tatttaatga cagttgcagc ctgaacatta gcggtagcga
    19141 tgaagagcag gcgcggcgag tgctggaaga gtatatccag gtgcgcttta tcgacagcga
    19201 tagcgtccag ccgacgctgg cagaactgac ggcgcatccg ctgccgcgtt cattaagccg
    19261 cctgaacccg gatttactgt acggcaacgt gctggcaagc ggcgttggcg tgggtacact
    19321 gaccctgtta cagagcgaca gcctcgacag ttatcgggtg atccctgcga gtgcgcaaga
    19381 ttccacccta ctggagcaca gtctggcaac gcttgccgag caactgaacc agcaattgcg
    19441 tgagcgtgac ggcgaaagca aaactatcct cagcgcccat ttgtcgctga ttcaggatga
    19501 tgaatttgca ggcaatatcc gtcacctgat ggcagaacag catcaggggc tgggggcggc
    19561 gatcatcagc aatatggagc aggtttgtgc caaactttct gcttctgcca gcgattatct
    19621 gcgcgagcgg gtcagcgaca ttcgcgatat cagcgaacag ttgctgcata tcacctggcc
    19681 ggaactaaaa ccgcgcaata atctggtgct tgaaaaactg accattctgg tggctgaaga
    19741 tttaacccca agccagtttt tgagcctcga tttaaaaaat cttgcgggca tgattctgga
    19801 gaaaaccggg cgcacctcgc ataccctgat cctggcccgc gcttcggcga tcccggtact
    19861 gagcggactg ccgctggatg cgattgcccg ttatgccggg caacctgcag tgcttgacgc
    19921 ccagtgcggc gtgctggcga ttaacccgaa tgacgcggtg agcggttatt atcaggtcgc
    19981 gcagacgctg gcggataaac gccaaaaaca acaggcgcaa gctgccgcgc agctggccta
    20041 ttcccgtgat aacaaacgta ttgatattgc ggcgaatatt ggcaccgctc tggaagcccc
    20101 aggcgcgttt gctaacggcg cggaaggcgt tgggctgttt cgtaccgaaa tgctctatat
    20161 ggatcgcgac agcgcgccgg acgagcagga acagtttgaa gcgtaccagc aggtgctatt
    20221 ggcggcgggc gacaagccga ttatcttccg cacgatggac atcggtggcg ataaaagcat
    20281 tccttacctc aacattcccc aggaagagaa cccgttcctc ggctatcgcg cggtacgtat
    20341 ttacccggaa tttgctggcc tgttccgcac tcaattgcgg gcgattttgc gcgctgccag
    20401 tttcggcaac gcccagttga tgatcccaat ggtccacagt ctcgatcaga tcttatgggt
    20461 gaagggtgag atccaaaaag cgatcgttga gcttaagcgc gatggcctgc gtcatgcaga
    20521 gacgattacg ctgggtatca tggtggaagt gccgtcggtg tgctacatca tcgaccactt
    20581 ctgcgatgag gtcgatttct tcagtatcgg ttctaacgat atgacccagt atctgtatgc
    20641 agtcgatcgt aataacccgc gcgtatcgcc gctgtataac ccgattacgc catcgttcct
    20701 gcgcatgttg cagcagattg ttaccgctgc gcatcagcgc ggcaaatggg taggtatttg
    20761 cggtgaactg ggcggtgaaa gtcgttatct gccgctactg ctagggctgg ggctggatga
    20821 gctaagcatg agtagcccgc gtattccggc agtgaaaagc cagcttcgtc aactggatag
    20881 cgaggcgtgt cgggaactgg cgcgtcaggc gtgcgagtgc cgcagcgcgc aggaaattga
    20941 agcgttactt accgcgtttg cgccggaaga agacgttcgc ccactgctgg cactagagaa
    21001 tatctttgtt gatcagtcct ttagtaataa agagcaggcg atccagttcc tgtgcggtaa
    21061 cctcggtgtt aacgggcgta ctgaacatcc gttcgagctg gaagaagatg tctggcagcg
    21121 ggaagagatt gttactactg gcgttggttt tggcgtggcg atcccgcaca ccaaatcaca
    21181 gtggatccgt cattccagta tcagcattgc ccggttggtg aaaccggttg actggcagtc
    21241 agaaatgggc gaagtcgaac tggtgatcat gctgacgctg ggcgctaacg aagggatgaa
    21301 tcatgtgaaa gtcttctcgc agctggcgcg taaactggtg aataaaaact tccgccagtc
    21361 gctgtttgcc gcgcaagatg cacaaagtat tctgacgctg ctggaaacgg aattaacctt
    21421 ctgacgttag ccctgaaaac gtgcgctata ctctcccggc gtcagaccaa actgacgccg
    21481 gaaaacgcga caaaaatagt cgctatccgg aaacccgcaa cgctgcgcca cttcatgaat
    21541 cgacagatga tatttttgca aaatcatcct cgcttttgcc attcgtaccc agcgaacata
    21601 ctcgataaaa cgcattgttc catgctgagc aaacagtttt gacagatgat ttggcgtgat
    21661 attaaaaaac tgcgccacgc tctcgcgggt gagcagctgg gcataattat cctgaatcca
    21721 gttacaaatg ctgtgataga gaaaatcgcc gcgcggttgc gaattgcccg gcggcatatt
    21781 caccacgttg cggcaaagat gcagcaaact taacaccagt ggctgaatga tattctgctc
    21841 cagcggcgaa cggctaagat gggtgagggc ggtaagcatc gcttcacctt caccgcgttg
    21901 caggctggga agctggatct ggcgagcagg atgtaacaac gacgtggtgc ggctatcgta
    21961 aaacgataac ccaagccagg tcggtgcgaa caccaggctt aacagcagca ccggtttgtt
    22021 gttgaccggt aaattagccg cgcgtgctgg aataaacagc atttctcctt ccaccatacg
    22081 ttgctgcttt gcttccagca gattgccgta ttcgccgcgt agcacgatat ccagacgggg
    22141 aaaatcaata cataaactgc ccactggcaa tgaggtaggc tggctggcaa accagacgcg
    22201 ccccagtagt tgcgggttaa gcaccaggcc gctgaacaga tcggcgaaaa attgttgatc
    22261 ggcgggcaag cctggtgctt tcatgaaagt tcccgattaa agatgcatta actggcggaa
    22321 ttctttaact ttgctgcagc tgaccggcac ttcaaaatcc agatctttca gccgcagaat
    22381 ataggtgtta ttaaaccacg gttcgatctc gcggattttg ttcagattga cacaaaatga
    22441 gcgatggcag cggaaaaaat gcgacggcgg cagcttgctg caaaactcgg taatgttcat
    22501 cggcattacg taggattcac gccgcgtata gacaaaggtc attttctcat gcgcttcggc
    22561 gtaatagata tcgttaatgg gcgtgacgat gattcgctca tctttcacca gattaatggt
    22621 gtcattctca cgcgttaccg tcgcggcagg cgtactgctg gtctgctgtt gttgccaggc
    22681 tgcttccagt ttttgcagca tcccggtaat gcgtgactcc tgatacggtt tgagaatgta
    22741 gtcaaacgcc tccagttcaa acgcttctac cgcatgttct ttccacgcgg tgataaacac
    22801 aataaacggt ttatgggcga actggctgat gttttgtgcc agcaacacac catccagcga
    22861 cggaatattg atatccagaa aaatggcgtc gacgcggtta tgctgcaaaa acttcaacac
    22921 gtccagaccg tcgtcaaagg tgccgacaat ctccatctgg ctgtgctctt taattagcca
    22981 gctcagttcc tgttgtgcca ggaattcgtc ttcaacaatg atgactttca caatatcact
    23041 ccggctcaaa gcaataaggt agcctgtgag gcgactgggg tgcgttggtt aggaatgtaa
    23101 aacgcaattt ccgttcctgg ttccaggcgg cggatatgca gcccctcgcc atacaataac
    23161 ttcacgcgat gatggacatt cagcaggcca attttattgc ccggcatttc gttcgcttcg
    23221 acccgctcaa tcacctttgg atcgatgccg tgcccggtat ctcgcaccgc aatgcgtacc
    23281 cgattaccgc actcagcaac gctgatggtg acaacgcctt tacctttgca aggctggata
    23341 ccgtggacaa tggcgttttc caccaacggc tggatcagca ggctgggaat gcagcaattc
    23401 acctcttcat caatatcata gataaccgtc agcttgtcgc caaagcgggc ctgctcaatg
    23461 gcaatatagt ctttaatttg atacagctct tttttgatat cgatttgctc atcgtctttt
    23521 aattcaatgt tatagcgcag atagcgcgac aaattaaaga tcagctggcg agcagtatcc
    23581 ggattcagac ggattgacga tgaaatagcg ttcaaagcgt taaacaggaa atggggatta
    23641 attttgcttt gcagggcgcg aagctctgcc ttatttgcca tttcacgtag ctgctcggcg
    23701 cgtgacacct caagttgcgt ggagatgatt tgtgacagac cgaccgccat ttcctgtaat
    23761 gacgaggtta tctgatgcgc gtggcagtag taaattttca gcgttccggt cacaacgcct
    23821 ttctcccaca atgggatcac cagcatggaa tgaatctctg gtgtgcggtg ggcttcatca
    23881 ttgtttttaa tgatgatttt cccgtaattc atcgcctgac gggtagtcgg gctaatgaag
    23941 tcatcgccat tctgatagtt atgttcaccc acgccaacgt aggccagcac atgatcggta
    24001 ttagtcatcg ccaccgcatc ggcgtgaatg tcatcgcgga taatttcgca gaccttgcgt
    24061 aatgactcgc tattgacatg gcgaaacagc ggcagcgttt tgttggcgat atccagcgcc
    24121 agcttggcct gccgcgcggc gctggcctct ttttctccct caacactttg taccagcaac
    24181 acaataaagc cgatacagac gctgccgagg atcattggaa tcccgatttt agagacgata
    24241 tcgatcccca atgcggtagt tggtgcccag acaatcacca gaatcatggt caacgtttca
    24301 cacaacatgc cgccgagaat accgacgcgc cagcgctgtg ctttggggat tttcaggttg
    24361 atccagcccg atatacaacc cgccagaatg ctggtgataa agcaggggat cgccgtcacg
    24421 ccgccgatgt caattaaata acggtgaata cccgcaatca cgccggtaat gatgcccacc
    24481 cacgggccaa acagaatccc gcctgacatc accgcgataa tacgtacgtt caccagcgag
    24541 ccttctacgg gaacgccgga ccaggtgctg aacagggcga acagcgagaa aatggcggta
    24601 acggcgagca actcttttgg ggagtgcgcc gacttgtgca acagttcgcg aaacaggcgg
    24661 atacggatga gaaagaacag gcagataagc attaacgctg cccgatcgaa gaccgccagc
    24721 agcatgttga atatttcgtg cacgggtgac tctgaataag gattaaacct ctatgatagg
    24781 taacctgaag gctgatgacc agcaggcctt ttttgaggaa aaaccacaat tttaaaaggg
    24841 gattgaaaga taaatggtgt attagcaatg catctcttga taat
//
LOCUS       DACADF010000068        24807 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.068, whole genome shotgun sequence.
ACCESSION   DACADF010000068 DACADF010000000
VERSION     DACADF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.068"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(142..747)
                     /locus_tag="ISB30_002563"
     CDS             complement(142..747)
                     /locus_tag="ISB30_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990180.1"
                     /translation="MPSDDDFNSRFKQEVARRLKDSLNLHRQLAEEVEPEDDETPVQS
                     PDEASPQPKVSHAPKKTAVRIRGIARKRAAEARKMAVAASSKRIPTATPSQQVTRTES
                     KILTEFTSVKQVNSLDTEHERKQLNNKDFEAEIALKKRYGKWFLIILAFQLLIMNGVF
                     IADGAGKLEFEDLTLQLYMGGTLTEVFGLVLVVTKYLFKRK"
     gene            complement(757..1245)
                     /locus_tag="ISB30_002564"
     CDS             complement(757..1245)
                     /locus_tag="ISB30_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP1990181.1"
                     /translation="MATNPREHARRVLDTFWCGRGFPVDPAKIAHDMGLDVFITDLPG
                     KVSGALIKQKDQDPAIFLNSDDNKVRQRFSCGHELGHYIARQASHSDEYEYVDLRGET
                     ASAGTHPDEIFANKFAAELLMPVDEVRKLHADGQPSYIMAHYFGVSDDAMTYRLKNLR
                     LG"
     gene            complement(1644..1877)
                     /locus_tag="ISB30_002565"
     CDS             complement(1644..1877)
                     /locus_tag="ISB30_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990182.1"
                     /translation="MGTLGNQPCRSRYEFDDFNLANRAAALTKLAKETGVSVDTLIKL
                     EIAMQLNRLNSILIENGDKMDENLAGIGEILQK"
     gene            2121..2762
                     /locus_tag="ISB30_002566"
     CDS             2121..2762
                     /locus_tag="ISB30_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990183.1"
                     /translation="MKTFYEDWPETFVSRLDMLRALDDRGSTRRLYLERTGAIFDALA
                     EEIRTVVAGHPEIDVSELDIGPLYRYYKRGEKGNPLADLLIELAPPTCERVRISPEVY
                     IIPYLFFALLIAQGADNDARDFFNMMMRPLIIAYRFKQLARYLGTKGGGRPQHRLKSE
                     AIELADRFFTENPTAPLSRGVQYISGIFVAKYSDPPAASTIRKWLIPIYRSDK"
     gene            2914..3093
                     /locus_tag="ISB30_002567"
     CDS             2914..3093
                     /locus_tag="ISB30_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990184.1"
                     /translation="MNIQESIGELPETCRAVIKRKDGHIVGVRVLTDDERIASLMAFL
                     ELAEIAGYTITPPDA"
     gene            3171..3767
                     /locus_tag="ISB30_002568"
     CDS             3171..3767
                     /locus_tag="ISB30_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAP1990185.1"
                     /translation="MAQHSFIRVSGGSLIPATPDTQRWLTERVKPGAVVYADFKQARN
                     PAFHRKFFSLLNLGFDYWHPSGGAISPAERELVHGYVKLLAYYGGHGDVMAELADQYL
                     LDESEKRAGSISAVKSFEAFRAWAIMEAGFYDVHQMPDGSLMRVPRSISFAAMDDLEF
                     GQLYSAVLDVLWNYILFRTFASQEAAENAAAQLLDYTS"
     gene            3764..4057
                     /locus_tag="ISB30_002569"
     CDS             3764..4057
                     /locus_tag="ISB30_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAP1990186.1"
                     /translation="MKKIDLRKAARSRACTVRIPGVCNHNPETSVLAHYRLAGTCGTA
                     IKPHDMQGAIACSACHDAIDGRTKTDYEHDSLLLMHAEGVFRTLAIWRDEEFI"
     gene            4057..4728
                     /locus_tag="ISB30_002570"
     CDS             4057..4728
                     /locus_tag="ISB30_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAP1990187.1"
                     /translation="MSNEYLLEYTRIKLRAALRDLSGGSKGQLEALCEHPPADKNAYP
                     RKHIHRVQLEDRTVDALVTPVYALESFSRRRPAPPMSDFEFADSSWRRSVNSLDASQQ
                     AWLRYCYGGNLAFKHQTAICEAVWSRYKGNIPASTQRKVVKRLLSLVWLSVQAVAAAN
                     KREDFKEMAGSTLAGMLSVSRSTWCETYSLHWVGMKEAVRALDEVALLATLHHYQNHL
                     DDVCV"
     gene            4841..5224
                     /locus_tag="ISB30_002571"
     CDS             4841..5224
                     /locus_tag="ISB30_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990188.1"
                     /translation="MSEPLTAGVAAGSAGVTFAALFPEATPAVMICALAGAALYVLSS
                     GQHRFWKQVIFALISFVGGVYCAETASAIITGILNAVLSHLNPPVTVKVSPAIGALVA
                     SVISVTSLLRIMSQARLWKSDKGMK"
     gene            5224..5496
                     /locus_tag="ISB30_002572"
     CDS             5224..5496
                     /locus_tag="ISB30_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP1990189.1"
                     /translation="MTLHSVLINANAIICLMLALRLMFFQKTGRYRFFISLTAYLAIL
                     SAAWIALRIFYGKYTQVDPAEFFLNLTICIAVWRARGNISKITGDR"
     gene            5496..5975
                     /locus_tag="ISB30_002573"
     CDS             5496..5975
                     /locus_tag="ISB30_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HAP1990190.1"
                     /translation="MTDPKWLIEARKNLGIREMKGKQHAAEIVQYWKDIKRGGIKDDE
                     TPWCAAFTGAMLERAGIRSTRFESANSYLDWGNELKEPAYGCIAILSRSGGGHVGFVV
                     GKNAAGDLMILGGNQADEVNIKAFPRSRVTGYRWPAGQTDVPQSLPLVNAEKSISEA"
     gene            5983..6177
                     /locus_tag="ISB30_002574"
     CDS             5983..6177
                     /locus_tag="ISB30_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990191.1"
                     /translation="MKKLLLAVAFFTLAGCTHSTYTEATRADGSSIKHVMIAPGTKIT
                     TANGGCIDSTGAEASCLGGK"
     gene            complement(6237..6482)
                     /locus_tag="ISB30_002575"
     CDS             complement(6237..6482)
                     /locus_tag="ISB30_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990192.1"
                     /translation="MNREKIEALMSVILKEADPKKFPEKLSEDAIVSFYRANSSYLNC
                     STLREWLSGEAEPSDEILQKIMVQNNWRATKWSNQLP"
     gene            6851..7417
                     /locus_tag="ISB30_002576"
     CDS             6851..7417
                     /locus_tag="ISB30_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990193.1"
                     /translation="MASQAEVAAHLLLSDRRLRDLAKLPGAPVPQGRGDWELDAWRHF
                     YIRYLRSNRRDTTSNDEPEAGDNSPEKNREQWLKNEERQERILMARVKRRILAKRYAP
                     IELISVAVSRVAVELRTRVESWPPRLKKVWPEMPQEASSVLREELAIALNELSDIRID
                     FSDYDVSDIERDLDRVESLARDDTDDGC"
     gene            7404..9266
                     /locus_tag="ISB30_002577"
     CDS             7404..9266
                     /locus_tag="ISB30_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HAP1990194.1"
                     /translation="MTGVEWADKYFYLPEGSSHIAGHWTTQPVQVVMLNMMTNDAIKI
                     VSVRKSARLGYTKILVAALLYFAEHKKRSAVVYQPIDDESDGFVADEVDPAIAEMPVI
                     QKIFPDWDKSNERNNLQRKEMSGAILDFRGASAPGNFRRLTKQVVEGDEVDGWPLEVA
                     KKGKGEGSPIELALVRIKGAAYPKAIFGSTPTVTGKSHIEMLEDAADLTFRFYLTCPH
                     CGEEQVLVFGFDGIEYGLKWDNSLQTNEAKSSSAYYQCCHCPEHFYYRDLEKMEFGGR
                     WIAEDCTWTRDGIHFFDHDGGVVRAPKHAAIVINALYSLNLDGWGEIVSEWLKAKGDP
                     LKEKTFHNTTLGELWSDVASEQLEHDILVNRREKYASQVPDGVVYITGGIDSQTSGRY
                     ECYVWGWGAEEECWLIDKTIVLGRYDEEDTLQRVDGVIRKQYRRSDGTTIGVSRWAWD
                     TGGIDAQVVYNRSLKLGPLWVIPIKGASSYGQPVVNMPRTRNANKVYLSLIGTDTAKD
                     LLAMRLQLEPDSKSATPGAIHFPNDDEIFSTTEAKQLVSEVLIPKLINGRVVYRWDNQ
                     GRRNEALDCWVYGLAALRISKIRFQLNLETLAEQRKKSQNKLSLEEMARMLGGS"
     gene            9266..9499
                     /locus_tag="ISB30_002578"
     CDS             9266..9499
                     /locus_tag="ISB30_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990195.1"
                     /translation="MTSREVLTERLLEAEIALHKLLTGKSTVSLSHGDSAGNNRSYQY
                     SQASIEQLRTYIIELKSQLGLSTGRRRPVGVRL"
     gene            9496..11070
                     /locus_tag="ISB30_002579"
     CDS             9496..11070
                     /locus_tag="ISB30_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP1990196.1"
                     /translation="MTAPQQLLGPDGKTPLRRYAGYNGGGPGFGGQLIDWNAPQQSAD
                     AALLPNFYRGNARADDLVRNNGVASNAVQLHQDHIVGNLFKLSYRPNWRYLGISREDA
                     RALARDVEVAWTEYAEDPHCTIDIERKRTFTMMIREGVATHAFNGETCVQPVWESSAG
                     SVFRTRFKMVSPKRIRNPGYAADTQFRRAGVDIDKNGAAVGYWIAEDTYPLGGVGKCR
                     RIPAQLSSGRHAFIHIFEPLEDGQTRGDNIFYSVMERLKMLDTLQQTQLQSAIVKAMY
                     AATIESELDSQQAFEYIAGAGTDIDSNPLNSFIQSYVTYYNGANIKLGGVKVPHLHPG
                     DKLSLQTAQNADAGFSSLEKSLLRYVAAGVGASYEELSRDYSQVSYSSARASANVSWR
                     FFMGRRRFIAARQASLMFCCWFEEALARGIITLPRSAVRSFYEARNSWTNALWIGAGR
                     MAIDGLKEVQESAMRITTGLSTYQNELALQGQDYEEVMEQQEYEIQRRREMGLSEPSW
                     SVSHPSNTNDNGWGGN"
     gene            11070..12377
                     /locus_tag="ISB30_002580"
     CDS             11070..12377
                     /locus_tag="ISB30_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HAP1990197.1"
                     /translation="MPWNNFPHLAARAFNQPLLLEPTYARVFFSALSDRFGTGRLIDT
                     ASGEVMNSDEMNALAMGWDSSERTRQKSYRVERGIAVLPVTGTLVHKLGYINPVSGMS
                     GYNGIAKRLQQAISDPDVRGVLLDIDSPGGEVAGAFDTADLIARAREQKPVWALASDT
                     ACSAAYLLASACSRRLITQTGTVGSIGVLMAHRCVEKALEIAGVDVTLIYAGAHKVDG
                     NPYSQLPGDVRDEFQLSINSTREQFAQKVSDYTGLKKSRVLATEAAVFIGADAIKSGL
                     ADQLVNYADAIAVMADALKPKMERFMSGTETTAETTTTEQTATTTTVAQVEFNTEQIR
                     ADAASSELARVMAIINCPEAVGREAQAKALAGVPGMTVGQAQVVLAAAPKTAQARTET
                     ALDTLMSTESPATIQDAGSTTATGTTANVSMLVAAGRSILGDE"
     gene            12377..12706
                     /locus_tag="ISB30_002581"
     CDS             12377..12706
                     /locus_tag="ISB30_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HAP1990198.1"
                     /translation="MTTETYSPDDFILGPDLVVTTVGHFQGGINVPRLTPIMLDATVG
                     TFKVWDGSVGKAIGLTATAVNTGSSSADCSYYKSGSFRYTAINWGTVTDVAKRKSAFA
                     GTPISVG"
     gene            12765..13799
                     /locus_tag="ISB30_002582"
     CDS             12765..13799
                     /locus_tag="ISB30_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HAP1990199.1"
                     /translation="MSDSFTTSELITATQQVFKFNPLFLKLFFRETYTFTSEEVFLDK
                     IPGKVNMAVYCAPMITGKVDRTRGYSTNHFKPGYTKPKHTINPNMSIKRAAGEQIGQP
                     ETPVERRAKIIMQNLLDEELSISQLEEFQAVQAVLYGKYTVSGSNIETYEIDMSRSAT
                     NNVTQSGSTAWSTQDAETYDPSDDIESYADLASGAVNVIIMDGKAWKQLKRFKKFWTA
                     LDTRRGSNSQLEVALKNLGDVVSFKGYYGDTALFVYKGQYLDPVTDTETRYMPDNTMI
                     LGNTKNRGLRTYGAIQDEDALKEGISEATRYPKVWTTTGDPAVTQTMTQSAPAMVLTD
                     ADAFVVVKIA"
     gene            13834..14253
                     /locus_tag="ISB30_002583"
     CDS             13834..14253
                     /locus_tag="ISB30_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP1990200.1"
                     /translation="MSTKTELLARIDDLSAQLGRELPRSGTIAELESIVAGAESELDI
                     LNEQSGDAGGIEITVNTASGTDNELAQSIASAIVSQSQEQELAPATRRVKLRNTLDVY
                     HYVNGRRVREIVAAGREIVVDSPEVADLIAADHVYAL"
     gene            14250..14630
                     /locus_tag="ISB30_002584"
     CDS             14250..14630
                     /locus_tag="ISB30_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990201.1"
                     /translation="MSYYDDLRAGDEEMIREWGRPVKLRGKTDPIIAIFNEPYARVDV
                     PHAGFITGTVTSLTALSDDVAGVVARDVVQVPKQRSIEPDGSVTWSGWTDYVVKEPQP
                     DGTGLTNIFLEPHTSSENSEYSKY"
     gene            14662..15342
                     /locus_tag="ISB30_002585"
     CDS             14662..15342
                     /locus_tag="ISB30_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990202.1"
                     /translation="MADLRSNAQMFDIDVSALEQLRVEISATQHQMLMVYNRALNRTA
                     KHMHRISAGMILTALAAKNHKAVDKRIKPFIKRRNFTKEGAGDLSSAKLWYGLNDFRV
                     SELKGRLQNPRRQKQPRDLETGLFLKTKKGARGATFTPKSAGLAMMSWPDSFVAKRYG
                     AKSVWIRLDRGGIEEARVPVHDALEDAIDDYIFENIGPVFMGFFEKDLRGRVKGNVHV
                     DPKTGKRL"
     gene            15339..15875
                     /locus_tag="ISB30_002586"
     CDS             15339..15875
                     /locus_tag="ISB30_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990203.1"
                     /translation="MSGLDAFDEYLDRVKGAVLQIPFIKTFGIYPEIPAGFETPALFL
                     EISNWSQSDESVPGSIQSVELSCNLYLLREFAADQYGLKSQNAALYMTSWINGRMFGP
                     GTKPAKFSDAEPCDWIKNGQSVGSHSVQCVSFTQVVGVGPDIFDYPSQGTLKNIYVGI
                     APDIGAEHEGDYYGPIGR"
     gene            15856..16758
                     /locus_tag="ISB30_002587"
     CDS             15856..16758
                     /locus_tag="ISB30_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HAP1990204.1"
                     /translation="MAQSDDEYAAAENARRLRDAVKRGTIAAVKMNPPRCRVSFGGEH
                     QSGWLQWFTHATSERVDWSAPSVGDPVTVVSEGGDTRNGVVMLGLHIDDKAPPSNDPH
                     DHVTAYCDGATMTYNTKNHTLTWQGVPDGVVKILGESEIEIFGRADVTINSENVVNIH
                     GGKLINADADIINVTATDTINAHADLVNVIATSSVSVTAANRISLTAQTISAWAPGGI
                     TLAGPTHITETLVVDKLATFRNDISVTGDNGGTGNITTRGSVLAGQEVQDRQGTITEV
                     RTTYNGHTHICPDGETQQPNQPMA"
     gene            16761..17102
                     /locus_tag="ISB30_002588"
     CDS             16761..17102
                     /locus_tag="ISB30_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP1990205.1"
                     /translation="MLGMDRNTGKLLSGTDHIRQSIVDILTTPLGTRVMLPEYGSKLF
                     DLVDNPTDPSLAMRIIMESAGAIARWEPRVRIDRINVLAVDIGKITILIIATDIETQQ
                     RLEFNNMELIF"
     gene            17099..18019
                     /locus_tag="ISB30_002589"
     CDS             17099..18019
                     /locus_tag="ISB30_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP1990206.1"
                     /translation="MITSTVQNSIVKTIDMSLLPPPAFVKTPLFSDVKSNLLSELQIL
                     YPQFNALLESDPAVKLLEIVAYREIIITARVNQGMLAVLLAFAKGSDLDQIGANFDCL
                     RLLITPANPDVIPPTEAVYESDDEYRHRIQLSWYARNTAGSTNAYNYFALSSDPDVLS
                     AQAYGPPVTQPGYVDMYVLSRTGDGTPPQSLLNTVNAALSPDDTRPLTDFVTVKPASN
                     LNYRVEAVIVAGLGPDQNVLLNGAQSDLAIYVDTQHKIGATAALSGIYDAIHRDGTER
                     VILISPTEDVIAGVGQAPYCTEIKLSVQMG"
     gene            18022..18648
                     /locus_tag="ISB30_002590"
     CDS             18022..18648
                     /locus_tag="ISB30_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HAP1990207.1"
                     /translation="MTSQSVLPPNAITPERALEVVLSHVGDLPGDIRIIKNPDLCPAN
                     LLPWLAWEYAVTYWNPDWSEQQKREIIKAAAWQNKHRGTRGAVERALLTVGFESKMRE
                     WFEATPRDDPYTFAIKIYLLKSMGLDLELLNTFIAQIFDAKNCRSLLKEINFETGVDG
                     EFFIAGTPYAKIGVNVPADGDGGVKINGGLYISGSPIVSLNVEIGPNG"
     gene            18641..19825
                     /locus_tag="ISB30_002591"
     CDS             18641..19825
                     /locus_tag="ISB30_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1990208.1"
                     /translation="MAENKIYAVLTDRGAQLEAAALASGVPVVLNKFVIGDANGNDDV
                     TPDPARTALIHETYRGDIKSAENSGNQVIFTLYVPPDTGGYTIREVGILTDKGELYSV
                     ARSPDILKPTDSNGALISITYKYTLAVSSTSTVNVVVYDNYVTPEEADKKYLQISKNL
                     SEIPANGETAQQAARKNIGIDGDVAYRDKGNAFTHPNTFKDDVIFEDVLSSKKQININ
                     KPGTDLTLFSFAGLSDVSGDYLDFSTRGENNQKVFYRLRGVHYDGLVLLCGGQTYKYW
                     NEANLKPVKTINGESPDENGNIDISLSPDPSPEIGVVGAVGTYALVAINHAPQILPGS
                     IWAGGDLYYASINNTGSYNTDIATKPFYDTPLIGSWRAMAYSPGGVGDIGTACFLAIR
                     IA"
     gene            19825..20214
                     /locus_tag="ISB30_002592"
     CDS             19825..20214
                     /locus_tag="ISB30_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990209.1"
                     /translation="MISVDSFRKITNPVFNDEHGLSINIICDIDGIGNDIPFTAMKND
                     CEEHGRVLYERALSGDYGSISKFVKKEQTQKLQTTLRTVGDLSIEAFSIQCAVDAGTA
                     SDAQIENLAELKEAIATRIGENREGIK"
     gene            20211..21713
                     /locus_tag="ISB30_002593"
     CDS             20211..21713
                     /locus_tag="ISB30_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1990210.1"
                     /translation="MSVTNFLHGIRTLEYDDGTKEINTVDISVIGIVGTAPDSSYPTC
                     ASLLWGSELADNLVEFSTVMPGADGNNWIVEIVDLGINGNVATPGYSTLPDGSRKLTM
                     ITDGAMTPSKMYDQNQQYKEGLQIGEYINVTFGGDHAGTGTVFALPPTNLSGGKDESF
                     KYNVPTLIAGSQKKAGLLGGSGTLPPAVSEILNQEDAIIVVVRVEEDSDETKMRQNVV
                     NGINALLTSAQINQVTPRILIAPDYSANDYIAEQLEVVTNKLRGVGYIDSPRGATPAD
                     VVNRRQRYGGRMEILRPRVYSTSDVSGLSRPYSAIAAGLRARIDNEKGFWWSKSNQNI
                     YGITGLEQVDDFIIGETNCTANLLNASQVSTIIRYDGFRHWGNYLCSLSPQWSFECVR
                     RTADVIEDSIARAMMTDFIDRPIDLHLGTDVVESINAYLHKLEEQGAINGGRAWLDEE
                     LNTKESLAAGNLYINVDFGPKSPAQTITLMYRINNDYTVEALASLFKETV"
     gene            21731..22243
                     /locus_tag="ISB30_002594"
     CDS             21731..22243
                     /locus_tag="ISB30_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1990211.1"
                     /translation="MADSNTYRAFALFVQGERVLNCTEYTPVDMKIIEDDFKTGAMDT
                     AITLDGGMEKMSASFKVSGSDSGVMGYLGLIPGAKTRFEIRSAYTDNYGVNFERIDTY
                     EGLITAITDDAQGTDSKSAVGQSVTIAPSYYKRVQNGKIIYEIHPAKMKRVINGVDVL
                     AGVARILHVY"
     gene            22256..22537
                     /locus_tag="ISB30_002595"
     CDS             22256..22537
                     /locus_tag="ISB30_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAP1990212.1"
                     /translation="MESLLDSMTITLSRPFIIKGESRDTITIREPKLRDRIMFSNDKS
                     GLEERTATMLARLANLEREDLYALPACDYDQMEAAFNELVKPPKDRHQI"
     gene            22646..24286
                     /locus_tag="ISB30_002596"
     CDS             22646..24286
                     /locus_tag="ISB30_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990213.1"
                     /translation="MAISQNFKTQVVFGGRIDPSFRRGTTELNDAIRQTSSTVGKLTK
                     SQDKLKDKIAAMKLAGKDVSDLSAQYQKLDRRIKATTQDQEALNTQLAKKQRLEKWTG
                     RAKGAAKWGGRAAAGAVRATGRGIKWGTLGAAGLIGGAAAGALAMNAETSEKLGLAKS
                     YGVGVEKYAAWENIGKAAGLNGENIGDLSEELTNKIGEIGNEKSLNPMLFQIGLTKKR
                     MAGWDREKQFNEVMRRISEMKDEKQAASLADQLMGGEANKIMTYMKATGKSWEQTMSD
                     AQKSNLLTKEGAEGAARAHVSVTNLWGSITSGLADTLGKIGGELAPTFDSVRETFTSW
                     FKDNQGGFVSTITEWVKPENMKKMWDGIVNFGEGCVKFGKIIWAVVKKLEWLIPDEKS
                     DEEQKTYNDEYNRAYQEFMDSGGKYSPNAGLTADNIAKAKAEEAVENMRHPERQEQAK
                     SQAESMLAFVNPLSGMVNKNTAPAALDSSTLNIDTLKQAVSTPAPEQNNKIEINIVGA
                     TDPQSIQQSAASGVLDGLRQAASSYNRGAMFDKPAAAG"
     gene            24322..24711
                     /locus_tag="ISB30_002597"
     CDS             24322..24711
                     /locus_tag="ISB30_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1990214.1"
                     /translation="MLGLGDFIFAISTVAYNKLQRSDAWRWAQQTRFGKNDALQITGR
                     PNPTITIDGKINALFLDGCGVGLLTDLRALGNTGEPQQLVLGTGEVKGYWVLRELTET
                     QNSFLMGGTPKSQDFSLTLEYYGASLD"
BASE COUNT         6651 a         5657 c         6500 g         5999 t
ORIGIN      
        1 tgacgttaga gcaaaagaat ggtgattttt ttgatgtgta ttacgttgga actgatggga
       61 ataactatct gttttcaacg cactgctaat ttatcaaggc cctttgatca gggccttcga
      121 aagactgttg gaccgagaga gttacttgcg tttaaaaagg tattttgtca cgaccagcac
      181 gaggccgaat acctctgtca aagtaccgcc catgtacaac tgtagtgtta aatcctcaaa
      241 ttccagctta cctgcgccgt cggcaataaa tacaccattc attatcagaa gttggaaagc
      301 aaggataatc aggaaccatt tcccgtatcg tttttttaga gcgatttctg cttcaaaatc
      361 tttgttgttg agttgtttgc gctcatgctc ggtatcaagt gaattcacct gtttcacaga
      421 tgtgaactcg gtcaagattt tactctcagt acgggtgact tgttgagagg gtgtagcagt
      481 tggaattctt ttgctagacg cagctacagc cattttgcga gcttcggcag ctctttttct
      541 ggctattccg cgaatacgga cagccgtttt tttgggggcg tgcgaaacct ttggctgagg
      601 tgaagcttcg tcaggagact gaaccggagt ttcatcgtcc tccggctcaa cctcttctgc
      661 taactgacgg tgtaggttga gggaatcctt cagtctgcgt gctacttcct gtttgaaacg
      721 gctgttaaaa tcgtcatccg atggcatttt aaagcgttac cccaacctca agttctttaa
      781 acgataagtc atcgcatcgt cagatacgcc aaaataatgc gccattatat aacttggttg
      841 accgtcagcg tgcagttttc tgacctcatc taccggcatc agaagttctg ctgcgaactt
      901 gtttgcaaaa atttcatcag gatgcgttcc ggcgcttgcg gtttcgccac gtaaatcaac
      961 gtactcatat tcatcgctat gcgatgcttg ccgggcgatg taatgcccca actcatgccc
     1021 acatgaaaaa cgttgacgaa ctttgttgtc atcactgttt agaaagatcg ctggatcttg
     1081 atctttttgt ttaatcagtg cgccagaaac tttcccagga agatcggtaa taaaaacatc
     1141 taatcccatg tcgtgagcaa ttttcgctgg atcaacaggg aaaccacgcc cgcaccagaa
     1201 cgtgtccagc actcgccggg cgtgctctct tgggttggta gccatagctc ctccagttac
     1261 tgcattacga ttattggttg aaatatagac agcatgttcc gtaccacatt cctgtaaaag
     1321 tgcaaattct gcacttctgg tacgatatta actgttagta gtaaagtctt ccagataaat
     1381 cactttaaaa tcttgtgaat tgcttgcttt ttacgctgta ttgtggatgg ttcaatgttt
     1441 ccagtaaaaa catacatgat aatcatgagt ttagtgtttt tttaggcgtc tatatgtttc
     1501 tggaaaatac aggtctaacc tgatcttatc gttgagattt tttgtactca gtatgcgtga
     1561 acgattcgac caggtgactg tgtacgtgaa gaagaccgcc cttaatattt taaatgtgca
     1621 acccctcaga agaggggcta ctctcacttc tgcagtatct cgccaatacc tgccagattc
     1681 tcgtccatct tgtcaccgtt ttcgataagg atggagttga gccgattcag ttgcatagca
     1741 atttcaagtt tgattaaggt atccaccgat acaccagttt cttttgctaa cttagtaaga
     1801 gccgcagctc gattggctaa attaaaatca tcgaactcat aacgacttct gcacggctgg
     1861 ttgcctaacg tacccatagt gattcctcat gatataaaga cccaacaata taaattacca
     1921 ggcacatcat aagatagggt tccagcccat attttcaaat atttgcaagt atcaccgttc
     1981 agtctaaaaa gttggatgat gatttaacga gccataacaa aaccctgcct cggaggtttt
     2041 ttctttttta tagaactcag cggcgtgaaa acgaataata aattgtgcaa atagtgcagt
     2101 ttttattatt cgaggtaatt atgaaaacct tctatgagga ctggcctgaa actttcgtta
     2161 gccggttaga tatgttacgg gcgttggatg atcgcggttc gacccggagg ctctatcttg
     2221 aacggaccgg ggcaatattt gacgctctgg cagaagagat acgtacagtc gttgccgggc
     2281 atcctgaaat cgacgtgagt gaacttgata tcgggccgct gtatcgctat tacaaacgtg
     2341 gagaaaaggg gaatccgctg gctgacttac ttattgagtt ggctccacca acttgtgaac
     2401 gggttcgtat atctcctgaa gtgtacataa tcccgtatct gttttttgca ctgttgatag
     2461 cgcaaggcgc tgacaacgat gcccgcgatt ttttcaatat gatgatgcga ccgttaatca
     2521 tcgcctaccg tttcaaacaa ctggcgcgat acctgggaac aaagggcgga ggacgaccac
     2581 agcacagatt aaaaagcgaa gccattgaac tggctgatcg tttttttact gaaaacccga
     2641 cagcgccatt atcgcgtggc gtgcaataca tatccggtat ttttgtggcg aaatactctg
     2701 acccacctgc agcgtcgacg attagaaaat ggttaattcc aatttacagg agtgataaat
     2761 aatgaccata aacggtttaa tcccctataa aaccgtttaa taaaattcac gacgtgaata
     2821 taattgttca ttattccctc attgtatttg gcgttatgac aaatgccata aaatactgta
     2881 taaatataca ggttgtggca ttggggggaa gacatgaata ttcaagaatc tataggcgaa
     2941 ctaccggaaa catgccgggc tgttatcaag cgtaaggacg gacacatcgt tggtgtgcgt
     3001 gttctgaccg atgatgagcg gattgccagc ctgatggcgt ttctcgagtt ggcagaaata
     3061 gctggatata ctattacacc ccctgacgcg taaaacacgg tataatatcg gtgctggatt
     3121 gaacacccgg caccattttc tgaacactgc cgcgccacct ggagttaacc atggcgcagc
     3181 attcatttat cagggtatcc ggcggttcgc taatacccgc gacaccagac acgcaacgct
     3241 ggttgactga acgagtcaaa ccaggtgctg ttgtgtatgc agatttcaaa caggcgcgta
     3301 atcccgcgtt tcatcgtaaa tttttctcac ttctcaacct gggctttgat tactggcatc
     3361 cgtccggagg ggctatttct cctgcggagc gcgaactggt tcacggctac gttaagttac
     3421 tggcgtatta cggtggacac ggtgatgtca tggcagagct ggctgatcag tatcttctcg
     3481 atgagtcgga aaagcgcgcg gggagtatca gtgcggtgaa gtcgttcgag gcgtttcgcg
     3541 catgggcgat tatggaagct gggttttatg acgttcatca gatgccagac ggcagtttga
     3601 tgcgcgtacc tcgttcaatc tcgtttgcag cgatggacga tcttgagttc ggtcaactgt
     3661 attcagccgt tttagatgtg ctgtggaatt atattttgtt ccgcacattt gcctctcagg
     3721 aggccgctga aaatgccgcc gcgcagctgc tggattacac atcatgaaaa aaatcgacct
     3781 gagaaaagct gcacgcagtc gcgcttgtac tgtgcgtatc cccggtgtgt gcaatcacaa
     3841 tcctgaaacc agcgtactgg ctcattatcg tctcgccgga acgtgcggca cagccatcaa
     3901 acctcacgat atgcagggcg ctatcgcctg cagtgcgtgt cacgatgcca tcgacggacg
     3961 tacaaaaacg gattacgagc acgactcatt gttgttgatg cacgctgagg gagttttcag
     4021 aacactggct atctggcgcg atgaggagtt tatctgatga gtaacgagta tttattggaa
     4081 tatacccgca taaaactgcg cgctgcattg cgggatttgt ctggtggttc taaggggcaa
     4141 ctggaagcat tgtgtgagca cccaccggca gacaaaaacg catacccacg caaacatatt
     4201 caccgtgtgc agctggagga ccggaccgtt gatgctctgg ttacgccagt ttacgctctg
     4261 gaaagtttca gcagacgtcg ccccgcgccg ccgatgagtg attttgaatt tgctgattca
     4321 tcctggcggc gttctgtgaa ctcgctggat gcaagtcagc aagcgtggtt gcgttattgc
     4381 tacggtggta acctggcatt caaacaccaa acagctattt gtgaggctgt ctggagtcgc
     4441 tataaaggaa acatccccgc gtcaactcag agaaaagtag ttaagcgcct gctttcgttg
     4501 gtgtggttgt ccgtgcaggc ggtcgcagca gcaaataaac gcgaggattt taaggagatg
     4561 gccggatcta ccctagcagg aatgctgtcg gtttctcgtt ccacctggtg cgaaacatac
     4621 tccctgcact gggtaggaat gaaagaggcg gtgagagcac ttgatgaagt ggcacttctt
     4681 gcaactttgc atcattatca gaaccattta gacgacgttt gcgtataatg cttgcaaaaa
     4741 cgaacaaaat aggccatatt tgacgctaat ttggtatgtt gccaaatttc taagaacctc
     4801 gccacggcgg ggttttgtcg tttctgaatc aggaaaaatc atgtctgaac ctctaaccgc
     4861 tggcgttgct gctggctcgg cgggggtgac gtttgctgcg ttatttcctg aggcaacacc
     4921 tgcagtgatg atctgtgcgc tggcaggcgc agctctctat gtgttgtcgt ccgggcagca
     4981 tcgattctgg aagcaggtta ttttcgcact catttcgttt gttggcggtg tgtattgcgc
     5041 tgagacagcg tcagccatca ttaccggtat tctgaacgcg gtgctgagtc acctgaaccc
     5101 tcccgtaaca gtaaaagtct ctcccgccat tggtgcactg gttgcatcag taatcagcgt
     5161 tacatcactg ttgcggatca tgtcacaagc tcgtttatgg aaatcagata aggggatgaa
     5221 ataatgaccc tgcactctgt cctcatcaat gccaatgcaa ttatttgtct gatgttggca
     5281 ctacggttga tgttttttca aaaaacaggc cgctatcgtt ttttcatctc actaactgct
     5341 tacctcgcga ttctgtctgc tgcgtggata gccctacgaa ttttttacgg gaaatatacg
     5401 caggttgatc ccgcagagtt ctttctcaac ctcaccatct gtattgctgt ctggcgggca
     5461 cgagggaata tttcaaaaat aacaggagac aggtaatgac ggatcctaaa tggctaatcg
     5521 aggctcgaaa aaacctcggc attcgggaaa tgaaagggaa gcaacatgct gcagaaattg
     5581 tgcagtactg gaaagatatc aaacgcggcg gcattaagga tgacgaaaca ccgtggtgcg
     5641 ctgctttcac cggggcaatg ctggaacgtg cgggtattcg ctcaacgcgt tttgagtctg
     5701 cgaattctta tctcgattgg ggtaatgaac tgaaggaacc cgcctatgga tgcattgcta
     5761 ttctgtctcg gtctggcggt ggtcacgtcg gctttgttgt cgggaaaaat gccgccgggg
     5821 atttaatgat tttggggggc aaccaggcag atgaagtaaa tatcaaagct tttcctcgtt
     5881 cccgcgtaac aggctaccgc tggccagcag gtcaaacgga tgttccacaa tcacttccat
     5941 tggtgaatgc tgagaaatct atctcagaag cgtaggtaaa acgtgaaaaa acttctctta
     6001 gctgtcgcgt tcttcacgct ggcaggctgc acacattcga catacaccga agcgactcgc
     6061 gctgacggca gcagtattaa acacgtgatg atcgcgccgg gtacgaagat tactactgcg
     6121 aacggtggtt gtattgattc aaccggggca gaggcttcat gccttggtgg aaaatagggt
     6181 aatttacttt aacgagaggc cgcctttttg ttttaaggcg gcacacattc aacagattaa
     6241 ggtaactgat ttgaccattt tgttgcgcgc cagttgttct gaaccataat cttctgaagt
     6301 atctcatctg aaggctctgc ttcgccgctt agccattctc ttaaagttga acaatttaga
     6361 taagaggagt tcgcacgata aaaagatacg atggcgtcct ctgataactt ctcaggaaac
     6421 ttttttgggt cagcttcttt taatattacg ctcattaagg cttcaatttt ttctctgttc
     6481 attttttgcg ttctcatggt tgttgttttt ctgtaatgat atcaataagt tcaagatata
     6541 tacatatatg gaaaacttgt tcggtagtct atcgatacag cacaaaagga tggctcgctg
     6601 ccaaagtgaa agcttacgct gataatcaga tacacaaagc tgatgtatgc aaacaagtac
     6661 gccgcgaggc tacagttgag tttgatgaac ctgatgcgag cccctaattc ctgtcctgaa
     6721 agggagatag ccccctggtc gcgggtcctt tccgaaatcc aaaacaccga gggtcggtag
     6781 acgcgcaaaa actcgctcgt ttttagtatt ttttcatttt gggtatttcc ggttccggtg
     6841 aggattttta atggcaagtc aggctgaggt tgcagcacat ttactgctat ctgatcgtcg
     6901 tctgcgcgat ctcgcaaaac ttccgggagc accagtccca caaggacgtg gtgattggga
     6961 gctagacgca tggcgccatt tttatattcg ttatctccgg agcaatagac gcgacacaac
     7021 tagtaatgac gaaccggaag cgggggacaa ttctcccgaa aaaaatcgcg agcagtggct
     7081 gaaaaatgag gaacgacagg agcgaatcct gatggcacga gtaaaacgcc gcattctcgc
     7141 taaacgctac gcgccaattg aattaattag cgtcgctgta tctcgcgtcg ccgttgaatt
     7201 acgtacccgc gtcgagtcgt ggccaccacg cctgaaaaag gtctggccgg agatgccgca
     7261 agaggcgagc agtgtattac gagaggagct ggcgatagcc ttaaatgagt tgtcagacat
     7321 acgaatcgac ttcagcgatt acgatgtcag cgatatcgaa cgcgatctcg acagggttga
     7381 atcccttgcg cgtgacgata ccgatgacgg gtgttgagtg ggctgataaa tatttttatc
     7441 tccctgaggg ctccagccac atcgctgggc actggacgac tcagccggtc caggtagtga
     7501 tgctcaatat gatgacgaac gacgcgataa aaatcgtgtc tgttcgcaaa tcagctcgtc
     7561 tcggttatac aaaaatactc gtcgcggcgc tgctctattt cgctgagcac aaaaaacgta
     7621 gtgccgtggt ctatcagcct atcgatgacg aatcggatgg atttgtcgcc gacgaggttg
     7681 accccgctat cgccgaaatg ccagtgattc agaaaatttt ccccgactgg gataaaagca
     7741 acgagcgtaa caatctccag cgtaaagaaa tgagcggcgc gattcttgat tttcgcggcg
     7801 cgagtgcacc aggaaatttc cggcgactaa cgaaacaggt tgtcgagggt gacgaagttg
     7861 acggctggcc gctggaagtt gccaaaaaag gcaaaggcga gggctcgccc atcgaactgg
     7921 cgctcgttcg aattaaaggg gcagcgtacc cgaaggcgat ttttggttcg acgccgaccg
     7981 ttaccggcaa aagtcatatc gaaatgctcg aagacgctgc cgatctgacg tttcgttttt
     8041 acctgacatg tccgcattgt ggcgaggagc aggtcctggt atttggtttc gacggtatcg
     8101 aatacggcct caaatgggat aacagcctgc agaccaatga ggcgaaatcg tcgtccgcgt
     8161 attaccagtg ctgccactgc ccggaacatt tttactatcg cgatctcgaa aaaatggagt
     8221 tcggggggcg ctggatagcc gaggactgca cctggacccg agacggcatt catttttttg
     8281 atcatgacgg tggcgtcgtt cgcgcgccga aacacgctgc gatcgtgata aacgccctgt
     8341 attcactaaa cctcgatgga tggggcgaga ttgtcagcga gtggctgaaa gcgaaaggcg
     8401 acccgctcaa ggaaaagacg tttcataaca cgacgctcgg cgaactctgg agcgacgtgg
     8461 ccagcgagca gctggagcac gatattctgg ttaatcgccg ggaaaaatac gccagccagg
     8521 ttcctgacgg tgttgtttat ataaccggcg gcatcgactc tcagacgtcc gggcgctacg
     8581 agtgttatgt gtggggctgg ggagccgagg aggagtgctg gctgattgat aaaacaatcg
     8641 tcctcggtcg ctacgacgag gaggacacgc tgcagcgcgt cgacggagtg attcgcaaac
     8701 aataccggcg cagcgacggg accacaatcg gcgtcagtcg ctgggcgtgg gataccggtg
     8761 gtatagatgc gcaggtcgtt tataaccgct cgctgaaact cggtccgctg tgggtcattc
     8821 caatcaaggg cgcgagctca tacggccagc cggtcgtaaa tatgcctcgt acccgtaacg
     8881 cgaataaagt gtatttgtcg ttgattggga cagatacagc gaaagacctg ctcgcaatgc
     8941 gcctgcagct ggaacccgac tccaaatcgg cgacaccagg tgcgattcat tttcccaacg
     9001 acgacgaaat attcagcacg acagaggcaa aacagctcgt ctccgaggtt ctgatcccga
     9061 aactcattaa cggtcgcgtc gtttatcgtt gggacaacca gggccggcga aatgaggcgc
     9121 tcgactgctg ggtgtacgga ctggcagcgc tgcgtatcag taaaattcgt ttccagctca
     9181 atctcgagac gctcgctgag caacggaaaa aatcacaaaa caaactgtct ctcgaggaga
     9241 tggccagaat gctcggaggg agctaatgac gtcgcgcgag gttttaacag aacgactgct
     9301 ggaagcggaa atcgctctgc acaaattatt aacgggtaaa tcgaccgtgt cgctgtctca
     9361 cggcgattca gcaggaaata accggagcta tcaatactcg caggcgagta ttgagcagct
     9421 ccgaacgtac attatcgagc tgaaatcaca gctcggtctg agtacgggac gccgtcggcc
     9481 cgtgggagtt cgattatgac tgctccgcag cagctgctcg ggcctgacgg taaaacgcca
     9541 ctacgccgtt acgcggggta taacggcggc ggtcccggtt tcggtggcca gctgattgac
     9601 tggaacgcac cacagcagag cgccgacgca gcactgttgc cgaattttta tcgcggtaac
     9661 gcgcgagcag acgatctcgt tcgcaataac ggcgtcgcgt cgaacgccgt gcagctgcac
     9721 caggatcata tcgtcggcaa tctgtttaag ttgagttatc gccctaactg gcgttacctc
     9781 ggcatatctc gcgaggacgc cagggcgctg gcgcgtgacg tcgaggtagc gtggactgaa
     9841 tacgccgagg accctcactg cacgattgat atcgagcgga aacggacgtt cacaatgatg
     9901 atccgcgagg gcgtggctac tcacgctttc aacggtgaaa cctgcgtaca accagtctgg
     9961 gagagtagtg ccggcagcgt ttttcggacg cgattcaaaa tggtctcacc gaaacggatt
    10021 cgaaatccgg gttacgcagc tgacactcaa tttcgccgcg ccggtgttga tatcgataaa
    10081 aacggggccg cggtcgggta ctggatagcc gaggatactt accctctcgg cggagtcggt
    10141 aaatgccggc gtatcccagc acagctcagc agcggcagac acgcattcat ccacatattc
    10201 gagccgctcg aggacggaca gacccgcggg gacaatattt tttacagcgt catggagcgc
    10261 ctgaaaatgc tcgacacact gcagcaaaca caactgcaga gcgccattgt gaaggcgatg
    10321 tatgccgcga caatcgaatc agagctcgac agccagcagg cgtttgaata tatcgccggc
    10381 gcgggtactg atatcgactc gaacccgctg aattcgttta ttcagagtta cgtgacgtac
    10441 tacaacggcg caaatatcaa gctcggtggt gtgaaagttc cccacctgca tcccggcgac
    10501 aaactcagtt tacagaccgc tcagaatgct gatgctggtt ttagttcact ggaaaaatca
    10561 ctcctgcgtt acgttgcagc tggcgttggc gcgtcatacg aagaacttag ccgggactac
    10621 agccaggtca gttactccag cgcccgggcc agtgctaacg tcagctggcg atttttcatg
    10681 gggcgtcgac gttttatcgc cgcgcgacag gcgtcgctga tgttctgctg ctggtttgaa
    10741 gaggcgctgg cacgcggaat tatcaccctg ccacgctcag ctgtccggtc gttttatgag
    10801 gcgcggaact cctggacgaa cgcgctctgg atcggcgctg gtcgcatggc gattgatggg
    10861 ctgaaagagg ttcaggagag cgccatgcgc atcacaactg gcctcagcac gtatcagaac
    10921 gaactggcgc tgcagggaca ggattacgag gaggttatgg agcagcagga atacgaaatt
    10981 cagcgccggc gtgaaatggg gctgagcgaa ccgtcatggt ctgtatctca cccctcaaat
    11041 accaacgata acggctgggg aggtaactga tgccgtggaa caattttccg cacctcgccg
    11101 ccagggcgtt caatcaaccg cttttgctgg agcccaccta cgcgcgggta tttttttcgg
    11161 cgctgagcga ccggttcggt accgggcgac tgattgatac agcgtctgga gaggtaatga
    11221 acagcgacga aatgaacgcg ctcgcgatgg ggtgggacag cagcgagcga acacgccaga
    11281 aatcgtatcg cgtggagcgt ggtatagccg ttctgccggt taccgggacg ctggttcata
    11341 aattgggtta tatcaaccca gttagcggga tgagcggtta caacggaatc gcaaaacgcc
    11401 tgcagcaggc gatttctgat cccgatgtaa gaggggtcct gcttgatatt gactcccctg
    11461 gcggtgaagt cgccggcgcg tttgatactg ctgatttaat cgcccgggcg cgagagcaaa
    11521 aacctgtgtg ggcgctggcc agcgatacgg cctgcagcgc tgcctatttg ctggcgtcag
    11581 cgtgttcgcg ccggctgata acgcagacag gcacagttgg ttcaatcggt gtcctgatgg
    11641 ctcaccgctg cgtcgaaaaa gcgctggaga ttgctggcgt cgacgtgacg ctgatttacg
    11701 ccggcgcgca caaagttgac gggaacccgt attcccagct gccaggcgac gttcgcgacg
    11761 aattccagct gagtattaac agcacgcgcg agcagttcgc gcaaaaagtc tcggattata
    11821 ccgggctgaa aaaatccagg gtgctggcca cggaagctgc agtatttatc ggcgcggacg
    11881 cgattaaatc tggtctcgct gatcaactcg ttaattacgc ggacgctatc gcagtgatgg
    11941 ccgacgcact gaaaccaaaa atggagcgct ttatgtctgg tacagaaacc acggcggaga
    12001 ccacgaccac agaacaaacc gcgaccacga ctactgtcgc gcaggttgag tttaacacgg
    12061 agcagattcg cgcggacgcc gcatcaagcg aactggcgcg tgtgatggcc atcatcaatt
    12121 gtcccgaagc tgttgggcgc gaggcgcagg caaaagcgct cgctggcgtc cccgggatga
    12181 cggtcgggca ggcgcaggta gtcctcgctg cagcacctaa aacggcgcag gcgcggacag
    12241 aaacggcgct cgatacacta atgagcactg aatcaccagc gactattcag gatgctggca
    12301 gtactacggc aacaggaaca accgcaaacg tctcgatgct ggttgctgca gggcgttcaa
    12361 ttttagggga tgaataatga ctacagaaac gtatagcccg gacgatttta ttcttgggcc
    12421 tgaccttgtt gtcacgaccg tcggtcattt tcagggcggg atcaacgtac cccggctgac
    12481 accgattatg ctcgacgcta ctgtcgggac gtttaaggtt tgggacggct cagtcggtaa
    12541 agctattggc ctcactgcca ccgcagttaa tactggctcc agttctgcag actgctcgta
    12601 ctataaatca ggctcgttcc gttacacggc gattaactgg ggtacggtta ccgacgtagc
    12661 taaacgcaaa tcagcatttg cgggtacgcc gatcagcgtc ggctgatagc aaaaaactca
    12721 aaacaagccg cctccgggcg gtttttttat aacaggatat ataaatgagc gattcgttta
    12781 ctacgtcaga actgattacc gcgacacagc aggtatttaa gttcaatccg ttgtttttaa
    12841 agctgttttt ccgtgagacc tacacgttta cgagcgaaga ggtttttctg gataaaatcc
    12901 cgggaaaagt gaacatggcc gtgtactgcg cgccgatgat caccggcaaa gttgaccgca
    12961 ctcgcggcta ttcaacgaac catttcaaac cgggttacac gaaacccaag cacacgatca
    13021 atccgaatat gagcatcaag cgcgcagccg gtgagcagat tggccagccg gaaacgccgg
    13081 tcgaacgtcg tgcaaaaatc atcatgcaga acctgcttga tgaggaactg agcatcagcc
    13141 agctcgaaga gttccaggcg gtacaggctg ttctgtacgg taaatacact gtttccggca
    13201 gcaatatcga gacctatgag atcgacatga gccgcagcgc gacgaataac gtcactcagt
    13261 ccggttcgac agcctggtcg actcaggacg cggaaacgta tgacccgagc gacgatattg
    13321 aatcctacgc ggacctcgcc tccggtgcgg ttaacgtgat catcatggat ggtaaagcct
    13381 ggaagcagtt gaagcgtttt aaaaaattct ggacggcact ggatacgcgc cgtggctcaa
    13441 acagccagct cgaggtcgcg ctgaaaaacc tgggcgacgt tgtcagcttt aaaggctact
    13501 acggcgacac tgcgctgttt gtctacaaag ggcaatacct cgacccggta acagacactg
    13561 aaacgcgtta tatgccggat aacacgatga tccttggcaa cacaaaaaac cgcgggctcc
    13621 gcacttatgg cgcaattcag gacgaagacg cgctgaaaga gggtatttcc gaagcgacgc
    13681 gctatccaaa agtctggacc actaccggtg atccggcagt gacgcaaaca atgacgcaat
    13741 ccgcgccagc aatggtcctc acggacgccg acgcgttcgt tgtcgtaaaa atcgcgtaag
    13801 agtcgaaagg ctcttttttg ttaaggaatc aacatgagca caaaaacaga attgctggcg
    13861 cgtattgacg atctgagcgc tcagctcggt cgcgaattac cgcgcagcgg gactattgcg
    13921 gaactggaat caatcgttgc cggcgctgag tcagagctcg atattctgaa cgagcaatcc
    13981 ggtgacgcgg gaggcatcga gattacagtg aatactgcgt ccgggaccga taatgaactc
    14041 gctcaatcaa tcgcgagcgc tatcgtttca cagtcacaag agcaggagct cgcaccggct
    14101 acgcgccgcg tcaagttgcg taacacgctg gacgtttatc actacgtgaa cgggcgtcgc
    14161 gttcgcgaga ttgttgccgc aggccgtgaa attgttgttg attcaccgga ggtcgcagac
    14221 cttatcgcag ctgatcacgt ttacgctcta tgagctacta cgatgacctc cgggccggcg
    14281 acgaggagat gatccgtgag tggggacggc ccgttaaatt acgcggtaaa accgacccca
    14341 ttatcgctat tttcaacgag ccttacgcgc gcgttgacgt tcctcacgct ggttttatta
    14401 ccggtacagt aacgagcctg acggcgctct cggacgacgt cgctggcgtt gttgcgcgtg
    14461 acgtcgtcca ggttccaaag cagcgcagca tcgaacctga cggctctgtt acctggtctg
    14521 gctggaccga ttacgtggtt aaagagcctc aacctgatgg cacagggctc acgaatattt
    14581 ttttagagcc tcacacgtcc agcgaaaaca gcgagtattc aaaatactaa gtgggacctc
    14641 ggtcccactt aagggggaac cgtggccgat ttacgtagta acgcgcagat gttcgatatc
    14701 gatgtttcgg cgctggagca gctcagagtc gaaatcagcg cgacgcaaca tcaaatgtta
    14761 atggtataca acagggcgct gaaccgaact gcaaaacata tgcaccgaat ttcagcagga
    14821 atgattttga ctgcactggc agctaaaaat cataaggccg tagataaacg aatcaagccg
    14881 ttcattaagc gccgtaattt tacaaaagaa ggggcaggag acctgagcag cgccaagctc
    14941 tggtacggtc tgaatgattt ccgggtatct gagctaaagg ggcgattaca aaatccacgg
    15001 aggcaaaaac agcctcgcga tcttgagacc ggcctgtttt tgaaaaccaa aaaaggcgct
    15061 agaggggcaa cttttacacc caaaagtgca gggctggcga tgatgagttg gcccgattca
    15121 ttcgtagcga aacgctacgg tgcgaaaagt gtctggattc gactggatcg cggaggaatc
    15181 gaagaggctc gcgtacctgt gcacgacgcg ctggaggatg ctatcgatga ttatattttt
    15241 gaaaacattg gccctgtttt tatgggcttt tttgagaaag atttgcgcgg tcgcgtgaaa
    15301 ggtaatgtcc acgtagaccc aaaaacaggt aaacgattat gagcggactg gatgcatttg
    15361 acgaatatct tgatcgcgtt aaaggcgcgg ttttacaaat accgttcatc aaaacattcg
    15421 gaatttatcc ggaaattccg gctggatttg aaacgccagc tctgttcctg gaaatcagca
    15481 actggtcaca gagtgacgaa tcggttccag gctcaattca gtcagtcgag ctgtcgtgta
    15541 atttgtattt gctgcgtgaa tttgcagctg atcagtacgg actgaaatcg cagaatgcag
    15601 cgctctatat gacgagctgg atcaatgggc gaatgttcgg tcccggaact aaaccggcaa
    15661 aattcagcga cgcggaaccg tgcgactgga ttaaaaacgg gcagtcagtc ggttcgcatt
    15721 cagttcagtg cgtgtcgttc acgcaggttg taggcgttgg tccagatatt ttcgattatc
    15781 cctcacaggg gaccctgaaa aatatatacg tcggaatagc accagatatc ggcgcagaac
    15841 atgagggcga ctattatggc ccaatcggac gatgaatatg ctgcagcgga gaacgcgcgc
    15901 aggctgcgtg acgcagttaa acgcggcacg atagctgcag ttaaaatgaa tcctccacgc
    15961 tgccgagtct catttggtgg cgaacaccag tcaggctggc tgcagtggtt tactcacgcc
    16021 acctcagaac gcgtcgactg gagcgcacca tcagtgggcg atcccgttac tgttgtttct
    16081 gagggtgggg atacgcggaa cggtgtagtt atgctcgggc tgcacattga cgacaaagct
    16141 ccgccaagca atgaccccca tgatcatgtc actgcatact gtgacggggc tacgatgacg
    16201 tataacacaa aaaatcacac tctgacatgg cagggcgtac cggacggcgt ggtaaaaata
    16261 ctcggtgagt ctgaaataga aatatttgga cgcgcagatg ttactattaa tagcgaaaat
    16321 gtagttaata tccatggtgg gaaattaatt aacgcagacg ctgatattat taatgtcaca
    16381 gcgacagata caattaacgc acatgctgat ttagtgaacg ttatagcaac gagttctgtt
    16441 agtgttactg ctgcgaacag aatatcgctg acagctcaaa caatcagcgc gtgggctccg
    16501 ggtggtataa cactagctgg tccaacgcat attaccgaga cattagtagt agataaatta
    16561 gcgacattcc gtaacgatat ttctgtcact ggagataacg gtggaacagg taatatcaca
    16621 actcgcggta gtgtgttagc agggcaagag gtgcaggacc gacaaggcac aataactgaa
    16681 gtccgcacaa cgtataacgg acacactcat atatgtccgg acggggaaac tcaacaaccg
    16741 aaccaaccaa tggcgtaaag atgcttggaa tggaccgtaa caccggtaaa cttttatcgg
    16801 ggactgatca cattcgtcag tctatcgttg atattttaac gaccccgctg gggacgcgtg
    16861 taatgctgcc ggaatatggc agcaaattat ttgatcttgt tgataatccc acagatccgt
    16921 cgcttgctat gcgaataatc atggaaagcg ctggcgcaat agcacgatgg gaaccacgcg
    16981 tcagaattga cagaataaat gtgttagcag tggatatcgg gaaaataaca atattaatta
    17041 tcgcaacaga tatcgaaaca caacagcgat tagagtttaa taatatggag ctgatatttt
    17101 gataacatca acagttcaga attcgatagt gaaaactatt gatatgagtc tgctgccgcc
    17161 gccagcattc gttaaaaccc cgttattttc agatgttaaa tctaatctac tgtcagagct
    17221 gcagatatta tatccacaat ttaacgcact cctggagtca gacccggccg ttaaactgct
    17281 ggaaatagtt gcatacagag aaatcattat tacagctcgg gtaaatcagg ggatgctcgc
    17341 tgtattgctc gcgtttgcaa aggggagtga cctcgaccag attggcgcta attttgactg
    17401 cctgcggctg ttgataacgc cagctaatcc cgatgtaatt cccccgactg aggctgttta
    17461 cgagagtgac gacgagtatc gacaccgaat acagctatca tggtatgcgc ggaatactgc
    17521 cggcagtacg aacgcctata actatttcgc gctgtcgagt gatcccgacg ttctgtcagc
    17581 tcaggcgtac ggcccgccgg tgacccagcc tggatacgtc gatatgtatg tcttgtcgcg
    17641 aaccggcgac ggcactccgc ctcaatcact actgaataca gtgaatgcgg ccctgtctcc
    17701 tgacgatact cgaccgctga ctgatttcgt gactgtaaaa cctgcgtcga acctgaatta
    17761 ccgggttgag gccgttatcg tagccgggct cggacccgac cagaacgttt tgcttaacgg
    17821 tgctcagagc gatttggcga tatatgtgga tacgcaacat aaaatcggag cgacggcagc
    17881 tctgtcagga atttacgacg cgatacatcg tgacggcact gaacgtgtga ttctgatatc
    17941 gccaacagag gatgtgatcg ctggcgtcgg gcaggctccg tactgcacag aaatcaaact
    18001 cagcgtgcaa atggggtagc catgacaagt caaagcgtac tgcctccgaa cgcaataacc
    18061 cctgagaggg cgcttgaggt tgttttatct cacgtcggcg atctgcctgg cgatattcga
    18121 attattaaaa atcccgattt gtgccctgca aatctgttgc cgtggctggc gtgggagtac
    18181 gcggtcacct actggaaccc cgactggagt gagcagcaaa aacgcgaaat tattaaagca
    18241 gctgcgtggc agaataagca ccgcggaacg cgtggagctg ttgaacgagc gttattaact
    18301 gttggctttg agagcaaaat gagagagtgg tttgaagcca cgcccaggga cgacccgtat
    18361 acattcgcaa ttaaaatata tctgctaaaa agcatggggt tagatttaga actattgaat
    18421 acatttattg cacaaatatt tgatgcaaaa aattgtcgtt ccttattaaa agaaataaat
    18481 ttcgaaacgg gtgtcgatgg tgagtttttt atagccggta caccttacgc gaaaataggc
    18541 gtaaacgtcc cagcagacgg cgacggcggt gtaaaaatta acggcgggct atatatatcg
    18601 ggctcaccaa ttgttagttt aaatgtggag atagggccaa atggctgaaa ataaaattta
    18661 cgcagtactg acagaccgtg gcgcacaact tgaagccgct gcgctggcat cgggtgtacc
    18721 cgtggtgctg aataaattcg ttattggcga cgcaaacgga aacgacgacg taacaccgga
    18781 cccggcacga actgcattaa ttcacgagac gtatcgtggg gatattaaat ccgcagaaaa
    18841 tagcggtaac caggttatct ttacacttta cgtaccgcca gacactgggg gctatactat
    18901 ccgtgaggtg gggatattaa cagataaagg cgagctgtac tctgtggcgc gctcgccgga
    18961 cattttaaaa cctacggaca gcaacggtgc gctgatttca atcacgtata aatacacact
    19021 cgcggtgtcc agcacatcta cggttaatgt tgtagtatat gacaactacg taactcccga
    19081 ggaagctgac aaaaaatatt tgcagataag taaaaactta tctgaaattc ccgcaaatgg
    19141 tgaaactgct cagcaggctg cgcggaaaaa tattggtata gatggggacg ttgcatatag
    19201 agataagggt aatgcattta ctcatccaaa tacttttaaa gatgatgtga tttttgaaga
    19261 cgttttatcc tctaaaaaac aaataaatat aaacaaaccg ggcactgatt tgacgttgtt
    19321 ttcatttgct ggtttatcag atgtgtcggg cgattattta gatttttcga cccgtggaga
    19381 aaacaatcag aaggtttttt atagattacg cggagttcac tatgatggcc tggtgttact
    19441 gtgcggcggg cagacgtata aatactggaa tgaggctaat cttaaaccgg ttaaaacgat
    19501 taacggggag tcgccggatg aaaatggcaa catagatata agtctttcac cggacccatc
    19561 accggaaata ggtgttgttg gcgcggttgg tacttatgcg cttgtcgcta ttaatcatgc
    19621 accacaaata ctcccgggct ccatatgggc tggaggggat ttatattacg ctagtataaa
    19681 taataccggt tcatataaca cggatattgc cacaaaacca ttttatgaca caccacttat
    19741 tggctcatgg cgagccatgg cgtattcacc cggtggagtc ggtgatattg ggactgcctg
    19801 ttttttagct attaggattg cgtaatgata agcgttgatt catttagaaa aataacaaac
    19861 cctgttttta atgacgagca tgggttatct ataaatatta tttgcgatat tgacgggatt
    19921 ggaaacgaca ttccattcac cgcaatgaaa aatgattgcg aggaacacgg tagggtttta
    19981 tatgaaagag cgctgagcgg ggattatggg agcatttcta aatttgtaaa aaaagagcag
    20041 acgcaaaaac tccagactac attacggact gttggcgatc tgtcaattga ggcattctca
    20101 attcagtgtg cagtagatgc cggaaccgca tctgacgcgc aaatcgaaaa tttggccgag
    20161 cttaaagaag caatagcgac gcgaattggt gaaaatagag aaggaataaa atgagcgtaa
    20221 ctaatttttt acacggtatt cgcacgctcg aatacgacga cggcacaaaa gagatcaaca
    20281 cagtagatat ttccgtgatt gggattgttg gtactgctcc cgattccagc tacccaactt
    20341 gtgcatcact attatgggga tcagagctcg cagacaatct tgttgagttt tcgactgtca
    20401 tgccaggggc cgacggaaac aactggattg ttgaaattgt tgatttggga attaacggga
    20461 atgtagcaac tccaggatac agcacactgc ccgacggttc gagaaaatta acaatgatca
    20521 cggacggtgc catgaccccg tcaaaaatgt acgatcagaa ccagcagtat aaagaaggac
    20581 tccagattgg agaatatatt aacgtcacat tcggtggtga tcacgcagga acggggactg
    20641 tattcgcgct ccccccaaca aatttatccg gtggtaaaga cgaatcgttc aaatataatg
    20701 ttccgacgtt aattgcaggc agtcaaaaaa aagctggatt gctcggtggg tctggaaccc
    20761 ttcctcccgc tgtatctgaa atactaaatc aggaggatgc cattattgtt gttgtccgcg
    20821 tcgaagagga cagcgacgaa acaaaaatgc ggcagaacgt cgttaacggg attaacgcat
    20881 tgttgacgtc tgcgcaaata aatcaagtaa cgccgagaat tttaatcgct ccagattata
    20941 gtgctaacga ttatattgca gaacaacttg aagtggtgac aaataaatta cgaggcgttg
    21001 gatatatcga ttcaccgcga ggcgctacgc ctgctgacgt tgtaaatcgt cgtcagaggt
    21061 acggcggcag aatggaaatt ctgcgccctc gtgtttattc tacgagtgat gttagtggtt
    21121 tatctcgtcc gtattcagct atcgctgctg gtttacgagc cagaattgat aacgaaaaag
    21181 ggttttggtg gagcaaatcg aatcaaaata tctacggaat tacaggactc gaacaagtcg
    21241 atgattttat aatcggtgaa acgaactgca cagcaaatct gctgaacgcc agccaggtca
    21301 gcacaattat tcgctatgat ggattcagac actggggtaa ctatctgtgt agcctcagtc
    21361 cccaatggtc ctttgaatgc gttcgtcgga cagctgacgt aattgaggat tcaatagccc
    21421 gggcaatgat gaccgatttt attgatcgcc cgatagacct gcacctcgga acagacgttg
    21481 tagagtcgat aaacgcgtat ctgcataaat tagaggagca gggcgcgatt aacggaggac
    21541 gggcgtggtt agatgaagag ttaaatacaa aagaaagtct ggccgctggt aatctttata
    21601 ttaatgttga ctttggaccg aaatccccgg cacaaacaat tacgttaatg taccgcatta
    21661 ataatgatta cacagttgaa gctctggcct ctctttttaa agaaacagtc taataatctg
    21721 gagaattgat atggcagata gtaacaccta tcgtgcattt gcgttgttcg tccagggaga
    21781 acgagtttta aattgcactg aatacactcc cgtcgatatg aaaataatcg aagatgattt
    21841 taagacgggc gctatggata cagctatcac tctggacggg ggaatggaga aaatgtcagc
    21901 cagttttaaa gtctctggct ccgattctgg tgtgatgggg tatttgggat taataccagg
    21961 tgctaaaaca aggttcgaaa ttcgaagtgc atatactgac aattacggtg tcaattttga
    22021 acgtattgat acatatgagg ggcttattac ggcgattact gatgacgcgc aggggactga
    22081 ttcaaaatca gcagttgggc aatcagtaac gattgcgccg agctattata aacgagttca
    22141 aaatggaaaa attatttacg agattcatcc tgcaaaaatg aaacgagtaa ttaatggtgt
    22201 tgacgttctt gcgggcgttg ctcgtattct ccatgtatat taaaaggcaa ataaaatgga
    22261 atcattatta gacagtatga ctattacgct ctctcgcccg tttattatta agggggagag
    22321 ccgcgataca attactattc gcgaaccaaa attacgcgac cgcattatgt tcagtaacga
    22381 taaaagtggt cttgaagagc ggacagcgac aatgctggcg cgcctggcaa atctggagcg
    22441 tgaggattta tatgcactgc ctgcgtgcga ttacgatcaa atggaggccg catttaacga
    22501 actggtaaag cctccgaaag accgacatca gatatagtta ttctgattcc gtttattgcg
    22561 aaaaaacttg ccatcccgcc cgatacgcaa atggacctgc cgtaccgggt ttttaatttc
    22621 tatgtaaacg aggtcatgaa aatcgatggc tatttctcaa aactttaaaa cgcaggttgt
    22681 attcggtggg cgaatagacc cgtcgtttcg tcgcggtaca acagaactca atgatgcgat
    22741 tcggcagacg tcgtctactg tcgggaaatt aacgaaaagt caggataaat taaaagacaa
    22801 aatcgcagca atgaaactgg caggtaaaga cgtttctgat ttatctgctc agtatcaaaa
    22861 gctggaccgt cgaataaaag cgacaacgca ggaccaggag gcgctgaata ctcaacttgc
    22921 taaaaaacag cggctggaga agtggacggg tcgcgcaaaa ggagctgcaa aatggggcgg
    22981 acgagccgcc gcaggtgcgg tgagggctac ggggcggggt atcaaatggg ggacacttgg
    23041 cgcggcaggg ctcattggcg gtgctgcagc tggtgcgctg gcaatgaatg cagagacgtc
    23101 agaaaaactc ggtctggcga agtcctacgg cgttggcgtc gaaaaatacg ctgcgtggga
    23161 aaatattggt aaagccgcgg gcctgaacgg cgaaaatatc ggggatttgt ctgaagaact
    23221 tacaaataaa atcggcgaaa tcggaaacga aaaaagcctg aatcccatgt tatttcaaat
    23281 tggcctgacg aaaaaacgaa tggctggctg ggatcgggaa aaacagttta atgaggttat
    23341 gcgtcgcatc tctgaaatga aagatgagaa gcaagcggcg agcctcgctg accagctcat
    23401 ggggggcgag gctaataaga tcatgacgta tatgaaggcg acgggtaaaa gctgggaaca
    23461 gacaatgtct gacgcgcaaa agtcgaattt gttaacaaaa gagggtgcgg aaggggcggc
    23521 gcgtgcgcat gtatcagtga caaatctgtg gggctctatt acgtcgggac ttgcagatac
    23581 actcggtaaa atcggggggg aactcgcgcc gacgtttgac tcagtacgtg aaacatttac
    23641 atcctggttt aaggataatc agggtggttt tgtcagcaca attactgagt gggtaaaacc
    23701 agaaaatatg aaaaaaatgt gggatggcat cgtcaatttt ggagaaggtt gcgtcaaatt
    23761 cgggaagatt atatgggctg tcgtaaagaa actggagtgg cttattccgg atgaaaaatc
    23821 agatgaagaa cagaaaactt ataatgacga atataatcgg gcatatcaag agtttatgga
    23881 ttcgggaggt aaatattcac cgaacgctgg attgactgct gataatattg cgaaggcaaa
    23941 agccgaagag gctgtcgaaa atatgcggca tccagaacgg caagaacagg caaaatctca
    24001 ggcagaatca atgctcgcgt ttgttaatcc attatccggg atggtaaata aaaataccgc
    24061 gcctgcagca ctggattcgt cgacgttaaa tattgatacg ttgaagcagg cagtctcgac
    24121 acctgcgccg gagcaaaaca ataaaatcga aatcaatatt gtcggtgcga cagatccgca
    24181 gtcaatacaa caatctgcag cgtcgggggt gctcgatgga ctgagacagg ctgcgagttc
    24241 gtacaatcgt ggcgcgatgt ttgataaacc agctgcggcg gggtgacaat gagtgatgca
    24301 ataaacggtg ctgaggatat catgctcggc ctgggtgatt tcatttttgc tatatcaaca
    24361 gtagcgtaca acaaactgca acgtagtgac gcctggcgct gggctcagca aacacgattc
    24421 gggaaaaacg acgcgttgca gataaccgga cgaccgaacc ccacaattac tatcgatgga
    24481 aagataaacg ctttatttct tgacgggtgc ggcgtcggcc tgttgacaga tttgcgagcg
    24541 ctcgggaaca cgggagaacc ccagcagcta gtgctgggga ccggggaggt gaaagggtac
    24601 tgggtgctgc gagaactaac tgaaacgcaa aacagctttt tgatgggcgg aacacctaaa
    24661 tcgcaggact tttcactcac gctcgaatat tatggagctt cgctagatta atacaaaatc
    24721 aagcgctacc agagcactta acaagtataa aaataataaa aaaaactacg aacataaatg
    24781 taaaacaacc tgatggtatt ttttttg
//
LOCUS       DACADF010000069        24710 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.069, whole genome shotgun sequence.
ACCESSION   DACADF010000069 DACADF010000000
VERSION     DACADF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.069"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..389)
                     /locus_tag="ISB30_002598"
     CDS             complement(<1..389)
                     /locus_tag="ISB30_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HAP1990215.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARTLGEQLLVGVSSDALNIAK
                     KGRAPVYPQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDD
                     FSCVCEVVYFPRTPSVSTTGIIEVIRG"
     gene            complement(392..826)
                     /gene="fliJ"
                     /locus_tag="ISB30_002599"
     CDS             complement(392..826)
                     /gene="fliJ"
                     /locus_tag="ISB30_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HAP1990216.1"
                     /translation="MRQIIDTLAQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAASVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(829..2169)
                     /gene="fliI"
                     /locus_tag="ISB30_002600"
     CDS             complement(829..2169)
                     /gene="fliI"
                     /locus_tag="ISB30_002600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HAP1990217.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESSGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTRLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPIAKKAEGQ"
     gene            complement(2162..2872)
                     /locus_tag="ISB30_002601"
     CDS             complement(2162..2872)
                     /locus_tag="ISB30_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein H"
                     /protein_id="HAP1990218.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTASISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPT
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALQETLTPESQGE"
     gene            complement(2876..3886)
                     /locus_tag="ISB30_002602"
     CDS             complement(2876..3886)
                     /locus_tag="ISB30_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAP1990219.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINHFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLGQIQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAERT
                     AE"
     gene            complement(3864..5510)
                     /gene="fliF"
                     /locus_tag="ISB30_002603"
     CDS             complement(3864..5510)
                     /gene="fliF"
                     /locus_tag="ISB30_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HAP1990220.1"
                     /translation="MNAQIKKLTQAFPAFRLHLVDNKRWVLMAGVGLAVAVTAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEDALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLQASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTRASVANVLDSLVGTGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPIAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTTQ
                     LTALLNNAAGIDAQRGDNLSLSLLNFVPQAVPVEPVIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPLMKRLTAVRAPVITPEPEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(5515..5856)
                     /locus_tag="ISB30_002604"
     CDS             complement(5515..5856)
                     /locus_tag="ISB30_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAP1990221.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPSMTFSSTDPDVSF
                     NRIMYGALGHVDQFQQVAEQQQTAVDTGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(5871..6857)
                     /locus_tag="ISB30_002605"
     CDS             complement(5871..6857)
                     /locus_tag="ISB30_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAP1990222.1"
                     /translation="MSELIATAASIINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENDNAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQDVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYATVLVKNITLSESTRRALLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPETDIADHSELQWPVQPAVHIAETRDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKQGIEVFS"
     gene            7244..8095
                     /locus_tag="ISB30_002606"
     CDS             7244..8095
                     /locus_tag="ISB30_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HAP1990223.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKSHNGFPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESSFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLAQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENNHE"
     gene            8088..8459
                     /locus_tag="ISB30_002607"
     CDS             8088..8459
                     /locus_tag="ISB30_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990224.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            8456..9208
                     /gene="fliP"
                     /locus_tag="ISB30_002608"
     CDS             8456..9208
                     /gene="fliP"
                     /locus_tag="ISB30_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016149597.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAP1990225.1"
                     /translation="MKRRTQLTLGLALLALAPLALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTLL
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GNAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            9211..9483
                     /gene="fliQ"
                     /locus_tag="ISB30_002609"
     CDS             9211..9483
                     /gene="fliQ"
                     /locus_tag="ISB30_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliQ"
                     /protein_id="HAP1990226.1"
                     /translation="MLTVDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            9485..10267
                     /gene="fliR"
                     /locus_tag="ISB30_002610"
     CDS             9485..10267
                     /gene="fliR"
                     /locus_tag="ISB30_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HAP1990227.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYMPVLDNSALTARVRI
                     ILSLALAIIITPLIPHPIPHNLLSLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            10257..11396
                     /gene="flhB"
                     /locus_tag="ISB30_002611"
     CDS             10257..11396
                     /gene="flhB"
                     /locus_tag="ISB30_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAP1990228.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPQVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFAAFLSLQALPFKLAINDGLSLYASVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDQSRAAAPFVVAKGTDEIALYIRQVATENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            11380..13473
                     /gene="flhA"
                     /locus_tag="ISB30_002612"
     CDS             11380..13473
                     /gene="flhA"
                     /locus_tag="ISB30_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAP1990229.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGAGAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RNQLLASPSVLYTATGIMFVLAVVPGMPHFPFLMFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTMTETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALM"
     gene            complement(13693..14190)
                     /gene="rayT"
                     /locus_tag="ISB30_002613"
     CDS             complement(13693..14190)
                     /gene="rayT"
                     /locus_tag="ISB30_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAP1990230.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLQNRRNQLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTQDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAEDVTDFSAG
                     ERIIL"
     gene            complement(14366..15115)
                     /locus_tag="ISB30_002614"
     CDS             complement(14366..15115)
                     /locus_tag="ISB30_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP1990231.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKANDRRWQA
                     LAENNHFLSSNHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            15427..16167
                     /locus_tag="ISB30_002615"
     CDS             15427..16167
                     /locus_tag="ISB30_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAP1990232.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(16138..16905)
                     /locus_tag="ISB30_002616"
     CDS             complement(16138..16905)
                     /locus_tag="ISB30_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAP1990233.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(17110..17688)
                     /gene="lpcA"
                     /locus_tag="ISB30_002617"
     CDS             complement(17110..17688)
                     /gene="lpcA"
                     /locus_tag="ISB30_002617"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAP1990234.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            17928..20372
                     /locus_tag="ISB30_002618"
     CDS             17928..20372
                     /locus_tag="ISB30_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAP1990235.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(20415..20888)
                     /gene="ivy"
                     /locus_tag="ISB30_002619"
     CDS             complement(20415..20888)
                     /gene="ivy"
                     /locus_tag="ISB30_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAP1990236.1"
                     /translation="MGRISPGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            21042..21812
                     /gene="yafV"
                     /locus_tag="ISB30_002620"
     CDS             21042..21812
                     /gene="yafV"
                     /locus_tag="ISB30_002620"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAP1990237.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESDSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFTFR"
     gene            complement(21911..21987)
                     /locus_tag="ISB30_002621"
     tRNA            complement(21911..21987)
                     /locus_tag="ISB30_002621"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21951..21953),aa:Asp,seq:gtc)
     gene            complement(22120..22851)
                     /gene="dnaQ"
                     /locus_tag="ISB30_002622"
     CDS             complement(22120..22851)
                     /gene="dnaQ"
                     /locus_tag="ISB30_002622"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP1990238.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGTCLWRA"
     gene            22916..23383
                     /gene="rnhA"
                     /locus_tag="ISB30_002623"
     CDS             22916..23383
                     /gene="rnhA"
                     /locus_tag="ISB30_002623"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAP1990239.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCKVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(23380..24102)
                     /locus_tag="ISB30_002624"
     CDS             complement(23380..24102)
                     /locus_tag="ISB30_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP1990240.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            24136..>24710
                     /gene="gloB"
                     /locus_tag="ISB30_002625"
     CDS             24136..>24710
                     /gene="gloB"
                     /locus_tag="ISB30_002625"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAP1990241.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLRKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKV"
BASE COUNT         5505 a         6631 c         6638 g         5936 t
ORIGIN      
        1 tgccccctaa tcacctcaat gatccccgtc gtcgataccg acggcgtgcg gggaaagtag
       61 accacctcac atacacacga gaaatcgtca aacttacctg cccagtcatc gcccatcacc
      121 aaaatatccg cactgtaccc gcgcaaatat tccgctttct gctccagcga ctcctccaga
      181 aatacgccat ccacgcacgc cagcccggcg ataatcgcca tgcggtcatc ctgagggtaa
      241 accggagccc tgcccttttt ggcgatattt aaggcatcgg atgatacgcc gaccagcagc
      301 tgctcgccca gcgtgcgagc gcgttgtaaa aggcgcagat gacccacatg aaacacatcg
      361 aaggtgccga aggtgatgac cgttttcatg gctactccgc taatttgtgc cgcaaccagc
      421 actggacggc gatgtcatcc accgcttttt gcgctttcgc cgtggcgctt tcctgttgct
      481 cgcggcgttt gtcgtccagc gtcagggcta ccactttctc ttcgcacgcc gccgccgtca
      541 ggtttttctg cattcgcgta gctttgatgt tggccagcgt tttttcctgc tcttgccagg
      601 cgatcacctt gcgcagtgtg cctttgtagc cgctgacgtt tttcagcgac tccaccgacg
      661 ccgccgcgcc cgcagatgtt ttctcgacaa ggtaacctaa tgctttgata ttgttatcat
      721 agccagcgca aatctgtttt tgctttgcca gctcgacggt tttgtctttg accgatttat
      781 cccgcaggcg ctgcaactgc gccagggtgt cgatgatttg tcgcatggtt attgcccttc
      841 tgcctttttg gcgatgggaa agaggatttg caggcggctt tgcaccagtt cgagggaggc
      901 gggttcgcgc atctcctggc gcaaaaatcg ctcgatagcc gggaacattt tcaccgcttt
      961 gtcgacgctg gcgtccgcgc ctgccacgta gccgccgagc gggatcagcg gtttgatctc
     1021 catataggcg gcgtagtttt gctttaacgc gcgggcggat tgctgatgtt ccaggcgggt
     1081 gacctggttc atgcagcggc tgatcgactg gccgatatca atagccggat agtgccccgc
     1141 ttccgccagt ttgcgggtta atacgatatg tccgtccagc actgcgcggg cgcagtcgac
     1201 tattggatct tgctggtcgt cgccttccgc cagcaccgtg tagatggcgg tcatcgatcc
     1261 tgagctttcg ctgttgcctg cgctctcgac cagtttgggg atcatgccga atgccgatgg
     1321 tggatagcct ttggtggcgg gtggttcacc gagtgagaga gcaatttcac gctgcgccat
     1381 ggcgtagcgg gtcagggaat ccaccagcag taaaacgtga tgcccgcggt cacgaaacca
     1441 ggcggcgatg gagtggcaca gttcggtggc tttcaggcgc atcagcggcg attcatcggc
     1501 aggcgcgaca acgacgatag actttgctag cccgtctgcg cccagcgagt ggtcgataaa
     1561 ctctttcact tcgcgcccgc gttcaccaat caacccgacc accacgatgt cggctttagt
     1621 ctggcgggtg atcatcccca gcagcacgct tttcccgacg ccgctgcccg ccatcagccc
     1681 gacacgctgg cctttgccga tggtcagcag gccgttaatc gctttcacgc ccacgtccag
     1741 cggttcgtcc actgaacggc gggttaaggg attgaccgag ggtggcagcg gcggcagaag
     1801 gtcgttgccg ttcaggcggc ctttgccatc cagcggttcg ccgagaccgt taaccacgcg
     1861 gcctaaccag ctttcgccaa tcaggatgtc gtgggctttt tcttccggga agacgcgtgc
     1921 gcctgccatc aggccgaccg gctgtttaaa cggcattaag taggtgatgt cgcgattaaa
     1981 gcccactgcc tgcgcgtcga tcagtgtgtg attggcgctt tcgatgcggc acagttgccc
     2041 ggtcattagc gggcagccga cgctctccag cagaatgccg ttcaggcgca ccagccgccc
     2101 ggcgacgcgg gccagcggga tcgactccag cgagcgcagg gcgttatcga aagcagaaaa
     2161 atcactcacc ctgcgactcc ggcgttagcg tctcttgcag cgcggtcatg cactgttcaa
     2221 ggcgatgctc acagccgatg tccagttcag atttgtcggt aatcacccgg cattcgcctg
     2281 gcggcagatc cggcgaaggc gtcagccccc attcgctgac tttttccggc accgcgtcgc
     2341 gcaggcggtt aaactcttct gtactcagca tcacttgcag ggtctccggc atcgtcggga
     2401 aggccgccag cgcctcttcc accagcgcca gtaactgcgt cggttgcagc gccagttcgc
     2461 agcggatcac ctgacgggtg actttctcca ccagttgcag caaatcttcc cgctgtttac
     2521 gttcaatatg cgcgaggaag tcgttcactt taccggtgat ggcttccagc ggctgcgccg
     2581 cctcaacaaa ctgtttgcgt ccttcctgct gcccggcgag gctgccttcg gtgtagccct
     2641 gacgccgccc ctcggcaaaa ccttgttgat gaccttcgct aaagccctgc tcctgacctt
     2701 cggtcattcc ctgagcaaac cctttttgca gcccttcctg aaaaccgtcc atcaactgac
     2761 gctggtaatc ggcggcgctg atactggcag taagcggttc tccggtcacc gcacgctggc
     2821 gcggcgggaa gcggtgcagg cgatagcgcc cgcgcagtgt ttcaatggtc atgaattact
     2881 ccgccgtccg ttccgcaaag agctgtagct gaatttcgcc cgcttcttcc agctcacggg
     2941 cgatgcccat gatttcgcgg cggatctgtt cgatacggct aaccggcacc ggacccagac
     3001 gcgcggtgat cgcttccagt tgctgcgcct gacgttttgg cattaccgcg taaatcgagc
     3061 ggcgcagcag ggcttcggtg cctttcagcg ccaccgccca gtcttccatc ggcacttcat
     3121 caagcagacg ccgacgcact tcttcgttct ggcggctgag gataaagaag tcgtacatct
     3181 catcctggat ctgccccaac acctcttcgt cgcgttcgcg catctggtcg aggatcacct
     3241 gctgattgcc ctggaagcgg ttgacgatat ccgccgcctg tttgatgcct tttaccttcg
     3301 agccatgttc cgaaagtacc gacaggccac gttcaatcag gcggtccagc tcatccacca
     3361 cgtcgcggtt gacgtcgttg agtttcgcca cccggtagag aatttcgttt tgcaccgatt
     3421 cgttgagata agagagcacc gctgccgaga tttccggcgt cagaaacgcg aggaacaccg
     3481 cctgcaattg cagatgttct tcggaaatca gcatcgccaa ctggcgtggt tccacccatt
     3541 gcaggcgcgc catccgcgag cgaatctcat cgccatagat gccgttgatc acgctgctgg
     3601 cgatctcggt acccaacgcc ttgttaagaa tgccctgcaa catggatcgc gatgcgccgt
     3661 tgatcccgct ctgttcgcgg aactcgtcaa agaagtggtt gatcacttta cgcgccatgc
     3721 tggttttgac gccggaaagt cgcgccatgt tttcgctcag gcgcaccact tcttcgcggc
     3781 tgagtttttg catcaccgtc gccgctgcct cttcgcccag acacagcagc aggatcgccg
     3841 cctgttccag gtagctgttg ctcttatttg ttgtcaattc gctcattgct ggtgatccat
     3901 tgtctgagaa cttcggctac gcgatcggtg tcgctcatgg ccagtttttg caggaactcg
     3961 agtttcactt cgaggccaga actttgtgac ggcaggctgt catcgccggg cagcgacggc
     4021 aggtcgacgt ttttgcgctc ctcttccggc atggcaatcc acggttcgct gacggcttcc
     4081 ggttctggtg tgataaccgg cgcacgtacc gccgtcagcc gtttcatcaa cggacgcacc
     4141 acgaagaaca gcagcaacag cgccagcagg ccgcagccaa tcagccgcac ccaggccagt
     4201 acgctgtcat ctttccacag tggaataacc ggttcgacag gcaccgcctg cggtacaaag
     4261 ttgagtaacg ataaggagag attgtcacca cgctgggcgt cgatcccggc ggcattgttc
     4321 agcaacgcgg tcagttgcgt ggtttgctcc ggcttccagt ttttcagcgc cggagcgttt
     4381 tgattgagta ccaccgccac gttgaggcgt ttcaccgcga agccgggatg ctgaatatgc
     4441 tggacgctgc ggtcgtaaga gtagtcgcgc ttactttcgc tgtgtttgga aagcgcttcc
     4501 ggcgagcggt tttcttccgt accgttggtc atctgattcg ctgcaatcgg cggacggtta
     4561 ctgagcgaac caggcacgcc catcgccacc tgattggtgt cgctgtccag cacgctctct
     4621 tcgcggttga ttttcggcgc gtcaccgtag tgttcctggg tttcgtcgat ggtgctgagg
     4681 tcgagatccg gcatcacact gacgcggtaa ttccccgtgc caaccagcga atccagcacg
     4741 ttcgccacgc tggcacgggt tttgtcctga atatctttga ggatctgatc gcgtttacgg
     4801 gtcgcggcag aaaccgcctc gcctgcgcca atgccgtcgg tcagcagatt tcccgcctga
     4861 tcgacgacgc taactttgct ggcctgtagc cccggaatac tgccggacac cagatgcaca
     4921 atggcgttca cctggtccat attcagcttc gcgccgtaat gcaagcgcac caccaccgag
     4981 gcgctgtttt gcggctcatc gctgaccaca aacgagcttt cttcgttgag cgccagatgc
     5041 acccgcgcgc tttccaccgc gtccagcgac atgatgctct gcgccagctc cccttcgaga
     5101 ctgcgtttat agcgcacgtt ctgcacgaac tgactgctgc cgagcacttc gtctttatcc
     5161 atcagctcgt agccgctggg caatatcgcc tgcacgcctt tcgcggcaag ggtcattcgc
     5221 gttttcgaca gtgcatcttc cggcaccaga atctgcccgc tctgcgggtc gatgcggtag
     5281 gtgagctttt cgccgtccag caccgtgaca atctgcgaca cgggcagatt ttcctggcgt
     5341 ccgtagagcg aaacgtagcc gcgattaccg gtccacagca cgctgacgat aatcgccgtc
     5401 accgccacgg caagccccac tcccgccatc aacacccagc gtttgttatc tacgagatgc
     5461 agtcgaaacg ccggaaacgc ctgggttaac ttctttattt gtgcattcat agggttagat
     5521 cgacatactc atcaggtcgt taaacccggt ggcgatttta ttgcgcacct gcaccagcgc
     5581 ggagaaagag agcgacgcct gctggctggc gatcatcgca cctgccaggt catcactttt
     5641 gcccgtgtcg acagccgttt gctgctgctc cgccacctgc tggaactgat caacgtgacc
     5701 aagcgcgccg tacataatgc ggttaaacga cacgtccggg tctgtggaac tgaaggtcat
     5761 ggagggtagc accggcgcct gggcgttggc ctgcatccgc tgcacgtcct gcatgatttg
     5821 cgcctgcatg gtgctgttga ttgtggtaat gctcattttt aacttccggg tcaggagaaa
     5881 acttcgatac cttgcttgcg catggaggcc agccgatagc gcagtgcgcg cggggtaatg
     5941 cctaacaggt cggcgatttt gctgcggttg ccctgatatt tacgcatcag gtcggcgata
     6001 tattgatact gcgcacttcg tccgtgctgg cctaaatcgc gggtttcggc aatgtggacg
     6061 gcgggctgaa caggccattg cagttcgctg tggtcggcaa tgtcagtttc cggcaggcct
     6121 aaggcatccg ggtaaatcac gccgtcgcgg tttaagatca ttcctctctg aatggcgttt
     6181 tccagttggc gaacatttcc gggccagcgg taattaagta gcgcccggcg tgtggattcg
     6241 gacagggtga tatttttcac cagcaccgtg gcatattttt taataaacga ctctgccagc
     6301 ggaataatat cgttcagacg ttcacgcagt ggcggcatgg ttataggaat aaccgccaga
     6361 cgataataaa gatcttcacg aaaacgtcca gcagccacgt cctgttccag atttttattg
     6421 gtgcaggcaa tcaggcgaaa attgagtttg atttgtctat tgctgcctaa tcgctcaacc
     6481 tgctgttcct gcaatacgcg taatattttg gcctgtaatg ccagcggcat atcgccaatt
     6541 tcatcgagca ataaggtgcc gttattagcc agttccattt ttcctggcac gctggcaatt
     6601 gcgccggtaa atgcgccttt gtcgtagcca aataaggtcg cttccagcat attttccggg
     6661 atcgccgcac agttcacgcc gatatagggc gcgttatcat tttcaccaaa ggctacggtg
     6721 tgaatatatt tcgccacgca ctctttcccc gcgccggttt cgccctgaat gagcaccggt
     6781 acgttaaagg cggcgacacg ctttgccaga gtaaaagcgt taatgatgga agcggcagtt
     6841 gcaataagtt cagacatagg gtgaacgata taagtagcga cagaaaacag gatgttctga
     6901 agcgtatcac cggtgggatt aacaagttcg aattaaacga taataaaaat attatttgtt
     6961 tataaacaac tagttagcaa tgtaggccgg ataaggcgtt tacgccgcat ccggcaattg
     7021 cgtgccgatg cctgatgcga cgctgcgcgt cttatcaggc ctacagtttg tgcaatgttt
     7081 tatgacgatg cccgatatct cgcccgttaa taatttaatt attttttcac cctattaata
     7141 atttgcccct ttcactcccc ctctatttaa tacctcacct tatgtcaccg ccggatttgc
     7201 gtgcacagaa acgcccatac cattacagca gagaaagtaa gccatgctga agtacagtaa
     7261 aacgccaggc atctttaaat tagaaggtaa tcggcttgga cgcccctacc atcgcctgcc
     7321 gaccctgttt acggggaatt ttgatgtaat tgattcgcat cttggcagct actttctcaa
     7381 aaagcatcgc agcaatatca ccctaaaaaa gattggctgc gaaatggata ttattaataa
     7441 aaatgccgag ctgatggttt cccaggtcgg gcatctggcg tttgatattg atcgctcctt
     7501 gctgttaatg ctgctgggca atttttatgg tctggagtcg tctcttgaag aagcgaaatc
     7561 acacaatgga ttcccgacca aaacggaaac acgtttaaaa aaccgcctcg cgctggatat
     7621 ctgcacgctg atttttaatc tgcaaacctc cggtattgcg ctgaaattaa aactggacag
     7681 cagcacggta attacccact gggcgtatca attaaccttt acccttgcgg gcgatgaaga
     7741 gagcagtttt cgtattttgc tcgacgacgc ccacaccgat tttattttga atctgatccg
     7801 ccacagcgag cacagtaaac cgcagcaggt ggcgaaatcc gttaataaac cggccctgat
     7861 taaagaaatc atccgctccc tgccgctgac cctgaacgtc aaaatcgccg agctatcgat
     7921 gaatgtcgcg gatctggcac agattaaagc cggggatatt ttacccatat cactgggaga
     7981 aacgttcccg gtggcgattg ggcaatctga attatttagc gccctgattg tggaagacaa
     8041 agacaaactg tttttgtcgg agctggcggg aaaaaatgag aacaaccatg agtaagcaag
     8101 aagatatttt agccggggat tttggcctca ccgatgaagt tgcgcctgtc gccaaatccg
     8161 ccgacgccga aacgctggtc acccgcctgg aagaccgatt ttccgactcg atgacgctac
     8221 tgaaacgcat tccggtgacc ttaacgctgg aagtatcgtc ggtggaaatc atgttagccg
     8281 acctgcttaa catcgacgac gacacggtga ttgaactgga taaactcgcc ggggagccgc
     8341 tggatattaa agtgaacaat atcctgctgg gcaaagcgga agtggtggtg gtcaacgaga
     8401 agtacggcct gcgcgtgctg gaatttaaca cccgcgatat caacgacctg gcgccatgaa
     8461 acgccgcact caactgacgc tgggcctggc gctgctggcg ctcgccccgc tggcgctggc
     8521 ccagggcggc gatatcgcac tgcttaacgt ggtcacccac ggcaacactc aggagtacag
     8581 cgtcaaaatt caggtactga ttctgatgac cctcgtcggt ttgctgccga cgatggtgct
     8641 gatgatgacc tgtttcacgc gttttattat cgtgctgtca ctgctgcgcc aggcgctggg
     8701 gctgcaacaa acgccgccaa accgcattct gattggtatt gcgctctcac tcaccctgct
     8761 ggtaatgcgc cccatctggc tcaatattta cgaccacgcg gtagtgccgt tcgaaaacga
     8821 tcagattacc ctgaccgatg cgttaagcac cgccgcgacg ccgctaaaac gctttatgct
     8881 ggcgcagacc gacaaaaaag cgatggcgca aatcatgacc atcggcggcg cgaagggcaa
     8941 tgccgccgac caggatctct ccatcgtggt gcccgcctat gtactgagcg agctgaaaac
     9001 cgccttccag attggcttta tgatttacat cccgtttctg gttatcgacc tgattgtcgc
     9061 cagcgtattg atggcgatgg ggatgatgat gctgtcgccg ctgattgttt cgctaccctt
     9121 taaactgatg ctgtttgtgc tgattgacgg ctggtcgctg accatcggca cgctcaccac
     9181 cagcattcgc gggctggggc tgggctgatt atgttgaccg tagacgtcgc cgccgacatt
     9241 gtcgccagcg gcattaaagt agtgattttg ctggtttcgg tactggtggt gccaagcctg
     9301 ctggtcggcc tgctggtgag cgtgttccag gcggtgacgc agattaacga acagaccctg
     9361 agctttttgc caaggctgat tgtcacgctg gtggtgctgg gcgtgtgcgg caaatggatg
     9421 attatccagt tgcatgatct ctgcattcac ctgtttaccc aggccgcgtt gctggttcat
     9481 tgagatgcgc acaagcgacg ttacgcaact gatggatctg gcgctggggc tgtggtttcc
     9541 cttcgtgcgc attatggcgt ttctgcgcta tatgccggtg ctggataaca gcgccctgac
     9601 cgcgcgcgtg cggattattt tgtcgctggc gctggcgatc atcatcacgc cgttgatccc
     9661 ccatcccatt ccccacaatc tgctatcgct caacagcctg atcctgacgg tggagcaaat
     9721 tttatggggc atgttattcg ggctgatgtt tcagtttttg tttctggcgc tacaactggc
     9781 cgggcaaatt ctctcgttca atatggggat gagcatggcg gtgatgaacg acccgagcag
     9841 cggcgcatcg accaccgtgc tggcggagct aatcaacgtc tacgcgatcc tgctgttttt
     9901 tgccatggac gggcatctgc tgctggtaag cgtgttgtac aagggcttca cctactggcc
     9961 gattggcaac gccctgcatc cgcaaaccct gcgtaccatc gcgctggcct ttagctgggt
    10021 gctggcgtcg gcatcgctgc tggcgctgcc gaccactttt atcatgctga ttgtgcaggg
    10081 ctgctttggc ttgctcaacc gtattgcgcc gccgctgaac cttttttctc ttggctttcc
    10141 gatcaacatg ctggcggggc tggtctgttt tgccaccctg ctctacaacc tgccggatca
    10201 ctatctgcat ctggcgaatt ttgtcttaca acaactcgac gcgctgaaag gtcactatgg
    10261 cggatagcag cagcgaagaa aaaacagaga aaccctcggc gcaaaagctg cgtaaagcgc
    10321 gtgaagaggg gcaactgccg cgctcgaagg atatggggct ggcggcgagc ctgtttgcgg
    10381 cgtttgtggt gatctccagc agttttccgt ggtatgccga ttttgtgcgc gagagcttta
    10441 tcagcgtgca tcagtatgcg caggagatta ataacccgca ggtgatcggc cagtttctgc
    10501 gccatcacct gttaattctc ggcaagttta tcctcacctt gctgccgatg cctgctgccg
    10561 cgctgctctc ttcgctggtg cccggcggct ggctgtttct gccaaaaaaa atcctcccgg
    10621 atttcagcaa aatcagtccg ttaaaaggca ttgggcggct gttttccagt gaacatctgg
    10681 cggaaacggg aaagatgacg gtgaagtctg tggtggtgct ggtgatgctg tgggtaagcc
    10741 tgcgcaataa ctttgccgcc tttctcagcc tgcaggcgct gccgttcaaa ctggcgataa
    10801 atgacggttt atcgctctac gccagcgtga tgcgcaactt tgtcattctg tttatcttct
    10861 tcgcgcttat cgacgtgccg ctggcgaaag ccctgttcac caaagggctg aagatgacca
    10921 aacaggagct gaaagaagag tacaaaaatc aggaaggtaa gccggaagtg aaggcgcgtg
    10981 tgcggcgact gcaacggcaa ctggcgatgg ggcaaatccg caaagtggtg ccgaaagcca
    11041 acgtggtgat caccaacccg acccactacg ccgtggcgtt acagtatgac cagtcgcgcg
    11101 ccgccgcgcc gtttgtggtg gcaaaaggca ccgatgaaat cgccctctat attcgccagg
    11161 tcgccacgga gaatcaggtg gaagtggtcg agttcccgcg gctggcacgc tcggtttact
    11221 acaccaccca ggtgaaccag caaattccgt ttcagcttta ccgggccatc gcccacgttc
    11281 tgacctacgt cctgcaaatg aaacactggc gcgagggcgc acagccgcgc ccggccctga
    11341 acagacatat ttccattccc aaagaggtgc ttaaactgga tggcgaaaac aactaaatcg
    11401 ttccttgcgc tgctgcgcgg cggcaatctc ggcgtgccgc tggtcatact gtgtattctg
    11461 gcgatggtta ttctgccgct gccgcctgcg ctgctggata ttctgttcac cttcaacatt
    11521 gtgctggcgg tgatggtact gctggtggcg gtgtcggcga aacgaccgct ggaattcagc
    11581 ctgttcccga ccattttgct gatcaccacc ttaatgcgcc tgacgcttaa cgtcgcctct
    11641 acgcgcgtgg tgctgttgca cgggcatctc ggcgcgggcg cggcgggtaa ggtgatagag
    11701 tcgtttggtc aggtggtgat cggcggcaac tttgtcgtcg gtttcgtggt gtttatcatc
    11761 ctgatgatca tcaactttat cgtcgtcacc aaaggggccg agcgtatttc cgaggtttct
    11821 gcccgcttta ccctggacgc gatgcccggc aaacagatgg caattgacgc cgatcttaat
    11881 gccgggttga tcaaccaggc acaggcgcag acccggcgca aagatgttgc cagcgaggcc
    11941 gatttttacg gcgcgatgga cggggcatcg aagtttgtgc gcggggatgc catcgccggg
    12001 atgatgattc tggcgatcaa cctgatcggc ggcgtctgta ttgggatctt caaatacaac
    12061 ctgagcgccg atgctgcctt ccagcaatat gtgttgatga ccatcggcga cggcctggtg
    12121 gcgcagatcc cttccctgct gctctccacc gcggcggcga ttatcgtcac ccgcgtcagc
    12181 gacaacggcg atatcgccca tgacgtgcgt aatcagttgc tggcaagccc gtcggttctc
    12241 tacaccgcca ctggaattat gttcgtgctg gcggtggtgc cgggaatgcc gcatttcccg
    12301 ttcctgatgt tcagcgccct gcttggtttt accggctggc ggatgagcaa gcgcccgcaa
    12361 gcggcggagg cggaagagaa aagcctcgaa acgctaaccc gtacgatgac agaaaccagc
    12421 gagcaacagg tcagttggga aactattccg ctgatcgaac ctatcagttt aagcctcggc
    12481 tacaaactgg tggcgctggt ggataaagcg cagggtaatc cgctcaccca gcggattcgc
    12541 ggtgtacggc aggtgatatc cgacggtaac ggagttctgc tgccggagat ccgtattcgg
    12601 gaaaacttcc gcctcaagcc cagccagtac gccattttca tcaacggcat taaggctgat
    12661 gaagcggata ttccggcgga taaactgatg gccctgccct ccagcgaaac ctacggcgag
    12721 attgacggcg tactggggaa tgacccggcg tacgggatgc cggtcacctg gattcaaccc
    12781 gcgcagaaag cgaaggcgct gaatatgggg tatcaggtga tcgacagcgc cagcgtgatt
    12841 gccacccatg tgaataagat tgtgcgcagc tatattcctg acctctttaa ctatgacgat
    12901 attacgcagc tgcataaccg tttgtcgtcg atggcaccgc gactggcgga agatttgagc
    12961 gcggcactca attacagcca gttgctgaaa gtgtaccggg cgctactgac tgaaggcgtt
    13021 tccctgcgcg atatcgtcac catcgccacc gtgctggtcg ccagtagtgc ggtgaccaaa
    13081 gatcatattc tgctggcggc agatgtgcgc ctggcgctgc ggcgcagcat tacccatccg
    13141 ttcgttcgca agcaggagct gacggtgtat acgctgaata atgagctgga aaatctgctg
    13201 accaacgtgg tgaatcaggc gcaacagggc gggaaagtga tgcttgatag cgtgccagtg
    13261 gacccgaata tgctcaacca gttccagagc acgatgcctc aggtgaagga gcagatgaaa
    13321 gcggcgggga aagatccggt gctgctggtg ccgccgcaac tgcgcccctt gctggcgcgc
    13381 tacgcaaggt tgtttgcgcc ggggctgcat gtgctgtcgt ataacgaagt gccggatgag
    13441 ctggagttga agataatggg ggcgttgatg taggcatccg gcatcggtgc atattgccgg
    13501 atgcggcgtg aacgccttat ccggcctacg ggtgagcacg gttttgtcgg catgataaga
    13561 cgcgtagcgg cgcatctggc accggtgcac aactgccgga tgcggcgtga acgccttatc
    13621 cggcctacgg gtgagcgcgg ttttgtcggc atgataagac gcatctgcgt cgcatcaggc
    13681 atcggcgcac agctataaaa tgatacgctc cccagcacta aaatccgtta cgtcctccgc
    13741 ccaatcgatg ggatataacc ctcgcgcgac atcgcgatga aacgttgaga atggccaatc
    13801 actcacttgc tttacccaac catgttttac tggatttata taaatataat caacatgatg
    13861 gcgatagtct tgggtattgc gaatggcatg ctcccaaaaa cgcggttgcc agatattttt
    13921 caatccacaa gcatgggtaa attgcttttt aatttcccgc cagcgcgagg aaaaatcatc
    13981 atcgccttca ggtaatgtcc atatgcaatg catatgctcc ggcaaaacta cccaagcgtt
    14041 aatttcaaaa ggtctgtctc gcttaacgtt aataatagcg ttacggagcg tctgaaactg
    14101 ggtggtcaaa agttggtttc gacgattctg taaatttacc gtgaaaaacc aggttccccc
    14161 cttgatgtaa taacgacgat attcagacat aacgactcct ttttctcaac ggtaagaaga
    14221 aaaggaaaag tcggcaatga tgttgtgtta aatcgggaaa gcggctcgca aaaaatgcac
    14281 agatttatag gcatgataag acgcgtagcg tcgcatcagg caccggtaca caaccgccgg
    14341 atgcggcgtg aacgccttat ccggcctaca aaatcgtctc ttccgtcaaa atccgccgcg
    14401 cgcccaaaaa atgatcctgc cagaaaggtt cagctaaccg acttacccga atggtttccc
    14461 cggtgcgcgg tgactcgata aactgaccgt ctccaagata tacgcccata tggtcggcta
    14521 tctcacggct gtggatatgg aaaaacagca aatctccccg gcgcaggtcg ttattcgcca
    14581 caatcgttgc ccgacggtag tggtacatct cattggccgt gcgcgggagc ttagcctcaa
    14641 ggatcttgtt gtaggcataa aaaaccaacc cgctacagtc gaaaccttta tcaggccgcg
    14701 tgccccccca gacataaggc ttgccaagtt gctgttccag gcggtgaatc gccacttcgg
    14761 tgatgttatg caggtggttg ctgctgagga aatgattgtt ttccgccagc gcctgccagc
    14821 gtctgtcgtt cgccttgagc ggcgcaggaa accattctgg atgctgtttt atctgctccc
    14881 gattatgctg gcgcagcgcc cttctgcttt tagcattgcc ttccacaata tagctggcct
    14941 gctttttcag atgggtttgg tactgtttta ataaacgcgc acggttttgc atccgctggc
    15001 gggcggcgta gctaaaagag atatgtgatg tggtggcgct ggcgagtaaa gggagcaaaa
    15061 cgcataacga aaacacgcct ggatgcagaa atctgccgtg caagaacgaa gacatgaagg
    15121 acataaacca gctgaatgaa ggtgagaatt ttaaaagacc ggaggagtaa acgggattat
    15181 taattgctta tttgcatatt actattgcgg tttcataatc ttaggaaata agaaataaat
    15241 ctacaatcca tgcgcttttc ggactcttga aaactgcacc tgtgctaaat ttgtaaaccc
    15301 tgcgccgaca ggcttgcgta aaactctttt ggtgataatg tgagccttcc aaatccagac
    15361 tattccgatt tgggattcat tttgccgtcc tggcgtgtgt aaccgtttta tcaaggaata
    15421 agcagtatgc gtaaaatcgc attaattctt gcgatgttgt tgatcccatg cgtttcgttt
    15481 gccggtttgc tgggcagcag tagctcgacc acgccagtga gcaaagagta taagcagcag
    15541 ttgatgggat cccctgtcta catccagatc ttcaaggaag aacgtacgct cgatctctac
    15601 gtcaaaatgg gcgagcaata tcaactgctc gacagctata aaatctgtaa atattccggc
    15661 ggcttagggc caaaacagcg tcagggcgat ttcaaaagcc cggaagggtt ttatagcgtc
    15721 cagcgtaatc agttaaaacc agacagccgt tactacaaag cgattaatat tggattccca
    15781 aacgcctatg accgtgcgca tggttatgaa gggaaatacc tgatgattca cggcgattgt
    15841 gtttccatcg gctgctacgc gatgaccaat cagggtattg atgagatctt ccagttcgtt
    15901 actggtgcgc tggtgtttgg tcagccgagc gtgcaagtga gtatttatcc gttccgcatg
    15961 accgacgcca atatgaagcg ccataaatat tccaacttta aggacttctg ggagcaactg
    16021 aagccgggct acgactactt tgagcaaacc cgtaagccac cgaccgtttc tgtggtcaat
    16081 ggccgttacg tggtcagcaa gccgttaagc cacgaagttg tgcagccaca actggcatca
    16141 aactacacgc tccccgaggc aaaataagcg ccattcacct ggcataatct tttgccaggt
    16201 ttcattgccc gtcagcggct gtgtcgcaat caccgtgacc acatcattcg gtgtggtctg
    16261 cgagctgaag tcgatttcca catcctgatc cagcaacgtt gccacgccaa acggcgcgcg
    16321 gcgggtgatc cagtgtaaat tagtcgagca atacgccatt acatagcgcc cgtcagaaag
    16381 cagcatgttg aaaacgccct tctgccgcag ttcatccgcc agtgaggcga tatatttaaa
    16441 cactgccgcc atgttgcccg gtgtgcgcgg gtaacgctgc gttaatttat gcaggagcca
    16501 gcaaaaggct ttttcgctat cggtttcacc gaccgggcgg aagttgccgg tttccagtga
    16561 tttgtagccc gtcagttgtc cattatgggc ataagtccag ttacgccccc ataactcgcg
    16621 ggtaaacggg tgagtatttt ccagcgccac ctcgccccga ttagcctggc gaatatgagc
    16681 caccaccgaa caggatttta tcgggtagtc ctggacaagt ttggcgatgg gggaattaaa
    16741 gctgggttgc ggatctttaa atgtgcgaca gcctttacct tcgtaaaagg taatgcccca
    16801 gccatcttta tgtggcccgg ttccaccacc acgctgtaca agtccggtga aactaaagca
    16861 gatatcggta gggacgttgg cactcatccc gagcaattcg cacatgggta acctccacaa
    16921 cagcgggagc gcatggcccc cggcgaagat tgatttaaga attttcaggt cggatgtggc
    16981 gtgaacgcat tattcggcct acggttcggg tgcagattgt aggcatgata agacgcgtca
    17041 gcgtcgcatc aggcatcaga gcacaaatgc cggatgcggc gtaaacgcct tatccggcct
    17101 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
    17161 aatgtggatc tcctgaatgc ggtcggcata accaaagtgc ggtacgcgaa tttcgatatc
    17221 cgccgtgcca gccattttgc cgccgtcttt accggtcagg gtgatcactt tcatcccctt
    17281 ctcacgcgcc gccgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
    17341 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtaa cgggagaaaa tatcattgaa
    17401 accaaaatcg ttaccgacgc aggaaatatg actaacgtca gaaatggcaa tcgccgggta
    17461 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tggcgtcgca
    17521 atgggagccg ccgttgccac aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
    17581 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
    17641 cgtttccgcc gcttcgttca gttcattacg aataagatcc tggtacatga ggatatcctt
    17701 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
    17761 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
    17821 agctttgttg taattatatt gtaaacgcat tgctaaatgt ttttacatcc actacaacca
    17881 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
    17941 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
    18001 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgcgggt ctgtggtcgg
    18061 cgtgggtact agtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
    18121 agtcgatgat ttccgcaccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
    18181 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
    18241 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gctgaagagc
    18301 aagcgtttct cgacggtccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
    18361 ctcatgagct ggcggacctg ccgccggagt tgtgggcgta ccttaaagag catcgtttct
    18421 tcgcgatgat catcaaaaaa gagtacggcg ggctggagtt ctcggcttat gcccagtctc
    18481 gtgtgctgca aaaactctcc ggcgtgagcg ggatcctggc aattaccgtc ggcgtgccga
    18541 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaaaatcact
    18601 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgacc agccccgaag
    18661 cgggttccga tgcgggcgcg attccggata ccgggattgt ctgcatgggc gaatggcagg
    18721 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctacattacg ctggcaccga
    18781 ttgcgaccgt gctgggtctg gcgtttaaac tctccgaccc ggagaaatta ctcggcggtg
    18841 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
    18901 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
    18961 tgttcgtgcc gatcgattac atcatcggcg ggccgaaaat ggccgggcaa ggctggcgga
    19021 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
    19081 gcgtgaaatc ggtagcgctg gcaacgggtg cgtatgcgca cattcgccgt cagttcaaaa
    19141 tttctattgg taagatggaa gggattgaag agccgctggc gcgtattgcc gggaacgcct
    19201 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggt gaaaaacccg
    19261 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcaa tcgattatcg
    19321 acgcaatgga tatcaccggg ggtaaaggca ttatgctcgg gcagagcaac ttcctggccc
    19381 gtgcttacca gggggcaccg attgccatca ccgttgaagg ggcaaatatt ctgacccgta
    19441 gcatgatgat cttcggtcag ggggcgattc gttgccatcc gtacgtgctg gaagagatgg
    19501 aagcggcgaa gaacaatgac gtaaacgcgt tcgataaact gctgttcaaa catatcggtc
    19561 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
    19621 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
    19681 acctcgccct gctttctgat gtctcgatgg cggtgctggg cggcagcctg aaacgtcgtg
    19741 agcgcatctc agcccgtctg ggggatattt taagccagct ttacctcgcc tctgccgttc
    19801 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
    19861 tacaggatgc gctgtatcag gcagaacagg cgatggacga tttactgcaa aacttcccga
    19921 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
    19981 caccgtctga caagctggat cataaggtgg cgaagatttt acaagtgccg aatgccaccc
    20041 gttcacgtat tggtcgcggt cagtacctga ctccaagcga gcataacccg gttggtttac
    20101 tggaagaggc gttggtggat gtgattgccg ccgacccgat tcatcagcgg atctgtaaag
    20161 agctgggtaa aaatctgcca tttacccgtc tggatgaact ggcgcacaac gcgctgacga
    20221 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
    20281 gtagtattaa cgttgatgac tttgatccag aagagctggc gacgaagccg gtaaagttgc
    20341 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
    20401 tttttatcgg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
    20461 aacgccgcga acagcaccgt tttgccatca atcgaaagcg catcattcac attcagccag
    20521 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
    20581 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgagg tttgcacgcg
    20641 ctcatcacct gatacgtctc atctcccaat gtcacggttt gtgcgggagt ataagtaccg
    20701 cctttcatca cccaggcagg cagcttatgc ccttgcacca tctgattaaa cgccgctttg
    20761 gtggtttcgc cctttgcaag actgctaatg gttaaatcat cctgcgccat tgcactggtg
    20821 gcgatgacca gagcggcgac tgtcgttatt gccttaaaca tcattcctcc cgggcttatc
    20881 ctgcccatgt gattaactcc ctaaagcctg gcgataacac gataatttgc aaatcagata
    20941 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
    21001 gttagagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
    21061 cgcttttgca gcaaccactg gtgtggatgg atggtcctgc caacctgcgc cattttgatc
    21121 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accggagatg tttaccagcg
    21181 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg aactggatga
    21241 ccgctaaagc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
    21301 ctgattcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
    21361 agcgtcatct gttccgcatg gcagatgagc atctgcatta taaagcaggc aatgagcgag
    21421 tgattgtgga atggcgcggc tggcgtattt taccgctggt gtgctacgac ttacgttttc
    21481 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
    21541 caccgcgctc tctgcactgg caggcattac tgacagcccg cgcgattgag aatcaggcgt
    21601 atgtagcggg atgtaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
    21661 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
    21721 gcattgatgc ggagctatcg atgatggcat tgcgggaata tagagaaaag tttccggcat
    21781 ggcaggatgc ggatgagttc acatttcgtt agagggtgaa tatggctacg agataagttt
    21841 cgtggctatt tttctctgga acttaaacga cgaaatcccg aatcagtaat gattcgggct
    21901 ttctgaatat tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg
    21961 tattctaacc gactgaacta ccgctccgcg ttgtgttccg ttgggaacga ggcgaatatt
    22021 acggattgcc tcacccttcg tcaacggttt ttctcatctt ttgaatcgtt tgctgcaaaa
    22081 atcgcccaag tcgctatttt tagcgccttt tacaggtatt tatgcccgcc agaggcaggt
    22141 tccgcccttc ttctgcacca gatcaagacg ggcttcatgt gctgcaagct cttcatctgt
    22201 cgcaaaaaca acgcgtaact tacttgcctg acgcacaatg cgctgaattg ttgtttcacc
    22261 ttgttgctgt tgtgtctctc cttccatcgc aaaagccatc gacgtttgac caccggtcat
    22321 cgccagataa acttccgcaa ggatctgggc atcgagtaat gccccatgca gcgttcgttt
    22381 actgttatct atttcgtagc gggcacataa cgcatcgagg ctgttgcgtt taccgggaaa
    22441 cattttcctc gccaccgcaa ggctatcggt gaccttacag aaggtattgg tcttcggaat
    22501 atcgcgctta agcaacgaaa actcgtagtc cataaagccg atatcaaacg ctgcgttatg
    22561 gatcaccaac tccgcgccgc ggatatagtc catgaactca tcggctacct cggcaaacgt
    22621 gggcttatcg agcaaaaatt catcggcaat accatgtacg ccaaaggctt ccggatccac
    22681 cagccgatcg ggtttgagat aaacatggaa gttattgccc gtcaggcgac ggttcaccac
    22741 ttcaacggca ccaatctcaa tgatcttgtg gccttcatag tgcgcaccaa tctggttcat
    22801 accggtggtt tcagtatcga gaacgatctg gcgtgtaatt gcagtgctca tagcggtcat
    22861 ttatgtcaga cttgtcgttt tacagttcga ttcaattaca ggaagtctac cagagatgct
    22921 taaacaggta gaaattttca ccgatggttc gtgtctgggt aatcctggac ctgggggtta
    22981 cggcgctatt ttacgctatc gcggacgcga gaaaaccttt agcgctggct acacccgcac
    23041 caccaacaac cgtatggagt tgatggccgc tattgtcgcg ctggaggcgt taaaagaaca
    23101 ttgcaaagtc attttgagta ccgacagcca gtatgtccgc cagggtatca cccagtggat
    23161 ccataactgg aaaaaacgtg gctggaaaac agcagacaaa aaaccagtaa aaaatgtcga
    23221 tctctggcaa cgtcttgatg ctgctctggg gcagcatcaa atcaaatggg aatgggttaa
    23281 aggccatgcc ggacacccgg aaaacgaacg ctgtgatgaa ctggctcgtg ctgcggcaat
    23341 gaatcccaca ctggaagata caggctatca agtcgaagtt taagcctgtg gtttacgaca
    23401 ttgccgggtg gcaccaaccg cctggcgaat tcgcggttta ttcttgctct gtttcatcgg
    23461 atttagcgtt aaaggaatag tccgtttccg ggcaacaata agttgtaagc aaccaagcgc
    23521 aggaatatgc gcattcaata gttttcctcc gtgtttgttc cacgggagaa cgtggaaacg
    23581 gctggcgtgt agcacttcaa aattcaacaa agagagccag tccagctgcc gcatcagagt
    23641 aaacatccgg ctgttatagg gcgatgtttt gcgcaatacc ggcacaagtt tgcgtaatcc
    23701 cattaaactg atgggattga agccactaat caccagccaa ccatcatcaa tcagtacccg
    23761 atcggcttca cgcaataaac gatgcggatc agtgcaccac ggcaatgtat gtgccagtag
    23821 acaaacatca acggatttat cggcaaaagg aagatgaagt gggtccgcct gcacctggac
    23881 gggcattcct tgcgcagaaa cattcacttg atgagaaacc gcgcacgctt cgcaattgat
    23941 ttctgcgctt aaattgccaa tcttaagcag atgaaaacca tacattttag tgaaccacgg
    24001 gttgagctga cgctccagcg ccttgcgata aagctctccc cagggcaaat cgccccagca
    24061 atcaggagcc acgacagttt gagggactct tgccggtttc atcacaacct tccgtttcac
    24121 actgagaggt aatctatgaa tcttaacagt attcccgcct ttgatgacaa ttacatctgg
    24181 gttttgaatg atgaagcagg tcgctgtctg attgtcgatc ccggggacgc agagccagta
    24241 ttaaacgcca tttccgccaa taactggcaa ccggaggcca tatttctcac ccaccatcac
    24301 cacgatcacg ttggcggcgt aaaagaactg gtggaaaagt ttccgcaaat tgtggtgtat
    24361 ggaccacaag agacacaaga taagggaaca acacaggtag tcaaagatgg cgaaactgcc
    24421 ttcgttttgg ggcatgaatt tagtgtaatt gccacaccgg gtcacacttt aggacatatc
    24481 tgttacttca gtaaacctta tctattttgc ggcgacacgc tgttttctgg tgggtgtggt
    24541 cggttgtttg aagggacacc atcacaaatg tatcaatcac ttagaaagtt aagtgcgtta
    24601 cctgacgaca cattggtatg ttgtgctcat gaatatacct tatcaaatat gaagtttgct
    24661 ttgagtattc ttccgcacga tttgtccata aatgattatt atcgtaaagt
//
LOCUS       DACADF010000070        24062 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.070, whole genome shotgun sequence.
ACCESSION   DACADF010000070 DACADF010000000
VERSION     DACADF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.070"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            134..2377
                     /gene="pdeF"
                     /locus_tag="ISB30_002626"
     CDS             134..2377
                     /gene="pdeF"
                     /locus_tag="ISB30_002626"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAP1990242.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLHIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNVPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2416..3957)
                     /gene="ppx"
                     /locus_tag="ISB30_002627"
     CDS             complement(2416..3957)
                     /gene="ppx"
                     /locus_tag="ISB30_002627"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAP1990243.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3962..6028)
                     /gene="ppk1"
                     /locus_tag="ISB30_002628"
     CDS             complement(3962..6028)
                     /gene="ppk1"
                     /locus_tag="ISB30_002628"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAP1990244.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            complement(6200..6838)
                     /gene="purN"
                     /locus_tag="ISB30_002629"
     CDS             complement(6200..6838)
                     /gene="purN"
                     /locus_tag="ISB30_002629"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP1990245.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6838..7875)
                     /gene="purM"
                     /locus_tag="ISB30_002630"
     CDS             complement(6838..7875)
                     /gene="purM"
                     /locus_tag="ISB30_002630"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP1990246.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8200..8826
                     /gene="upp"
                     /locus_tag="ISB30_002631"
     CDS             8200..8826
                     /gene="upp"
                     /locus_tag="ISB30_002631"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP1990247.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8912..10201
                     /gene="uraA"
                     /locus_tag="ISB30_002632"
     CDS             8912..10201
                     /gene="uraA"
                     /locus_tag="ISB30_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP1990248.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10251..10997
                     /gene="hda"
                     /locus_tag="ISB30_002633"
     CDS             10251..10997
                     /gene="hda"
                     /locus_tag="ISB30_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAP1990249.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11135..11494)
                     /gene="arsC"
                     /locus_tag="ISB30_002634"
     CDS             complement(11135..11494)
                     /gene="arsC"
                     /locus_tag="ISB30_002634"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAP1990250.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11515..12978)
                     /gene="bepA"
                     /locus_tag="ISB30_002635"
     CDS             complement(11515..12978)
                     /gene="bepA"
                     /locus_tag="ISB30_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAP1990251.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13191..14252
                     /locus_tag="ISB30_002636"
     CDS             13191..14252
                     /locus_tag="ISB30_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP1990252.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14350..14820)
                     /gene="bcp"
                     /locus_tag="ISB30_002637"
     CDS             complement(14350..14820)
                     /gene="bcp"
                     /locus_tag="ISB30_002637"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAP1990253.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14820..15392)
                     /locus_tag="ISB30_002638"
     CDS             complement(14820..15392)
                     /locus_tag="ISB30_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAP1990254.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15538..16416
                     /gene="dapA"
                     /locus_tag="ISB30_002639"
     CDS             15538..16416
                     /gene="dapA"
                     /locus_tag="ISB30_002639"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP1990255.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16433..17467
                     /gene="bamC"
                     /locus_tag="ISB30_002640"
     CDS             16433..17467
                     /gene="bamC"
                     /locus_tag="ISB30_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAP1990256.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17680..18393
                     /gene="purC"
                     /locus_tag="ISB30_002641"
     CDS             17680..18393
                     /gene="purC"
                     /locus_tag="ISB30_002641"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP1990257.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18561..19424
                     /locus_tag="ISB30_002642"
     CDS             18561..19424
                     /locus_tag="ISB30_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAP1990258.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19439..21454
                     /locus_tag="ISB30_002643"
     CDS             19439..21454
                     /locus_tag="ISB30_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAP1990259.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLYQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGGNEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPTRQREGTGRQLIAGALQYIHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVRTERLRDRITQWQFFH"
     gene            21528..22226
                     /gene="ypfH"
                     /locus_tag="ISB30_002644"
     CDS             21528..22226
                     /gene="ypfH"
                     /locus_tag="ISB30_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP1990260.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVNAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22307..22507)
                     /locus_tag="ISB30_002645"
     CDS             complement(22307..22507)
                     /locus_tag="ISB30_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAP1990261.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22535..23662)
                     /gene="dapE"
                     /locus_tag="ISB30_002646"
     CDS             complement(22535..23662)
                     /gene="dapE"
                     /locus_tag="ISB30_002646"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAP1990262.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFINELVAIE
                     WDQGNDFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23666..24022)
                     /locus_tag="ISB30_002647"
     CDS             complement(23666..24022)
                     /locus_tag="ISB30_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAP1990263.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         5595 a         6000 c         6337 g         6130 t
ORIGIN      
        1 tatcaattgg aactttcata tgacacttag aatcattgct ctctatagtg atgaataatc
       61 atcattcgaa gtcaggtggg atgcctgtct gaatacacct ccttcaggat gtgggggatt
      121 ctgctgagca tctatgaaac tgaatgcaac ttatataaaa atacgtgata aatggtgggg
      181 gcttccgctg ttcctgcctt ctttaatctt gcccattttc gcccacatta atactttcgc
      241 gcatatttct tccggtgaag tttttctctt ttatctgcca ctggcactga tgatcagcat
      301 gatgatgttt ttcagctggg cggcattgcc agggatcgcc ttagggattt ttgtccgcaa
      361 atatgcagag ctgggttttt acgaaacgct ctcattaacg gctaatttta ttatcattat
      421 cattctctgt tggggcggtt acagggtttt taccccccgg cgtaacaacg tttcacatgg
      481 tgatagccgt ttaatttccc agcgactatt ctggcagatt gtgtttcctg caacgctgtt
      541 tctgatactt ttccagtttg ctgcgtttgt agggttactg gcgagcagag aaaatctggt
      601 cggcgtcatg ccctttaacc tcgggacctt aatcaattat caggccttgc tggtgggtaa
      661 tctgattggt gtcccgctgt gctactttat cattcgggtg gtgcgaaatc cgttttattt
      721 acgtagctat tattcgcaat taaaacagca ggttgatgcc aaagttacca aaaaagagtt
      781 cgcaatctgg ctactggcat taggtgcctt attgttgctg ttatgcatgc cgttaaatga
      841 aaaaagcacg atttttagca ccaattacac cttgtcatta ttgctgcccc tgatgatgtg
      901 gggagcgatg cgctatggtt ataagttgat ttcattgctc tgggcggtcg tgttgatgat
      961 cagcatccat agctatcaaa attacattcc catttatcct ggctatacca cgcagctgac
     1021 cataacctcc tccagttatc tggtattctc ttttattgtc aattatatgg ctgtactggc
     1081 aacccgtcag cgagctgtag tcagacgcat tcagcggctt gcgtatgtgg acccggtggt
     1141 tcatctgcca aatgttcgcg ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct
     1201 atgctattta cacatccctg gcatggaaat gctggttaag aactatggca tcatgctgcg
     1261 gattcaatac aagcaaaaac tttctcactg gctgtcacca ttgctggaac cgggtgaaga
     1321 tgtttatcag ctttcgggta acgatctcgc gctgcggctg aatacagaat cgcaccagga
     1381 gcgcattacc gcactggata gccatctcaa gcaatttcgt ttcttttggg atggaatgcc
     1441 gatgcaaccg cagattggcg tcagttactg ctatgtgcgc tcgccagtga atcatatcta
     1501 cctgctgctg ggagagctaa atacggtggc cgaactttcc atcgtgacca acgtcccgga
     1561 aaatatgcag cgtcgcggag caatgtattt gcaacgcgaa ttgaaagata aagtcgcgat
     1621 gatgaatcga ctacagcagg cgctggaaca caaccatttt ttcctgatgg cccagccgat
     1681 taccggtatg cgtggtgatg tttaccatga aattcttctg cgcatgaagg gtgagaatga
     1741 tgagctgatc agccccgata gcttcttacc ggtcgcgcac gaatttggtt tatcgtcgag
     1801 tatcgacatg tgggtcattg agcatacgct gcaatttatg gctgaaaaca gagcgaagat
     1861 gcccgctcac cgttttgcta ttaatctgtc tccaacctcg gtatgtcagg ctcgttttcc
     1921 tgttgaagtc agtcagctgc tggctaaata tcagattgaa gcgtggcaac ttatttttga
     1981 agtcaccgaa agtaatgctc tgaccaatgt taagcaggcg caaatcacct tgcagcatct
     2041 tcaggaatta ggctgccaga ttgcgattga tgatttcggc accggctacg ccagctatgc
     2101 gcggcttaaa aatgtgaatg ccgatctgct taaaattgac ggcagtttta tccgcaatat
     2161 tgtgtcaaat agtctggatt atcagatagt ggcgtcgatt tgccacctgg cgcgaatgaa
     2221 gaaaatgcgg gtagtggcag agtacgttga aaacgaagag atccgcgagg cggtgctctc
     2281 tttggggatc gattatatgc agggttatct tattggtaag ccgcaaccgt taattgatac
     2341 gctgaatgaa atcgaaccca ttcgcgaaag tgcctgaata atgcgggccg acatttctcg
     2401 tcggcccgca aagcattaag cggcgatttc tggtgtactt tcttcttcaa ttttcaaccg
     2461 ccagccagcc acgccttccc agtattcttg ctccttttcc agatcaagca gtaccagcgc
     2521 attctgacta aaccagtcat gcgggaaacg cagtgtccag tgactgtcat cagtaatcag
     2581 cgtcaatgtt ggcggtgtgg tggttgcctg acgttgatta ttgagtaata cgccaaggcg
     2641 caatagctga atcagtggca ggaactgttt cttcttaaac aaggtaaagc gcggcagatc
     2701 gtcgagttta atcgctttac ggtgatagcg caccagtgtc gccatcatca gctgctgttc
     2761 ctgattaaaa cccggcaagt cgctgttttg cagaatataa gcggagtggc gatgcaaacc
     2821 gctgtggttg atattcaacc cgacctcatg cagcatggcg gcccatcgca gtagcgcctc
     2881 cagttgtgga tgcgccagct tcggttgctg ttcccgccac tgttcgtaca tttgcatagt
     2941 ggtatccagc acccgtcggg cctgttcgct gtcgatgtga tactggttgg cgaggctgct
     3001 ggcagtgcga ctacgcacat cctgatgacg gaaacgtcct tccatttcat acagtacgcc
     3061 ttcgcgtaac gccccgtcag aaaggcgcag ttcacggatg gctaaagcat caaacacacc
     3121 gcataaaatc gccagtcccg gaacgaagac tgttttccgt tcttcggaaa gacccggtaa
     3181 actcagcgat gcgaaattac ggtgacgtaa aacttctttt accagttttt ccagacgttc
     3241 cggggtaatt atcccgtctt tctcgcccat ttccattaac acttcatggg cggcttttat
     3301 ggtaccggaa gcgcccattg ccacgttcca gccctgaatt cgaaattgcc aggttaacgt
     3361 ttccagtttt tgtgctgccg ccatgcgagc gcgctgaaaa ttctctttat tgatgacccc
     3421 gccagggaaa taaagctggg caaagctgac acaacccatc cggcggcttt caacgagaat
     3481 cggttcgaaa ttttcaccaa tcaccagttc tgtagaaccg ccgccaatat caataaccag
     3541 tttgcgacct ttttccggtt gggtatgttc cacgcccata aaaatcagac gggcttcttc
     3601 attaccggaa ataatttcga tcgggtaggg aatgactttt tccgcgcgtt tcagaaagtc
     3661 agtggcgttc agcgcctgac gcagcgtatg ggtaccaact atacagacgc tggcagggga
     3721 aaacccttgt agcctttcgg caaatagcga cagacagttc aaaccgcgcg tcattgcctc
     3781 ttcactcaac atattatctg gccccaggcc gtccgccaga tgcacccgct gtttcaggcg
     3841 gccaataatc tgcatggcac catctaccac acgggctatg accatgtgaa aactgtttga
     3901 accaagatcg accgcagcaa actcctgcgg acgaggggat ttatcgtgta ttggcatagg
     3961 gttattcaga ttgttcgagt gatttgatgt agtcgtaaat cgccaactgc gcccgtactt
     4021 tgcggcgatt gccgcgggga acgtagcgat tactgagttc tttatcgata taacgtgctt
     4081 tgaccgtatc gctgaacaat atgtcgatga tgtccagcac ccgctgcttc aggcgcggat
     4141 cgagcagcgg cgttgccact tcaatacgat aatcaatatt gcgcgtcatc cagtcggcgg
     4201 aagaaaggta gaccttttta tcgccgccat tttcaaaaat ataaacccgg tcatgttcaa
     4261 ggtaacggtc aacaatactg atggcacgaa tgttgtcgct aatgccttcc agattgggga
     4321 tcagagaaca cattccgcgc accagcagat taaccggtac gccggagctg gaggccgcat
     4381 acagacgatc aaccagacct ttatcgacaa ggttatttag cttcagggtg ataccactgg
     4441 gcagcccttg ctgcgcgttg gcgatctcgc ggtccaccat ttcatacaac agacggcgag
     4501 agttttgcgg cgacaccatt aaataatcaa atgtcaccgg acggtatggg ttttcaataa
     4561 agttgaacac ccgccgtact tcgttagtga tgcgcgcatc ggcggtcagc aacgaatagt
     4621 cagtataaag acgcgcggtt ttttcgttaa agttcccggt cccgatgtgt gcgtaacgca
     4681 ccacttcacc gttttcttta cgtgaaatca ggaacagttt ggcgtgaatt ttcagccccg
     4741 gcgcagagaa gataacgtgc acgcctgctt cggtcaggcg cttcgcccag tgaatgttgg
     4801 cttcttcgtc gaaacgtgcc tgtaactcaa ccaccacggt gactttctta ccgttgtgtg
     4861 cggcgtggat catcgagtcg atgatgcgtg aatctttcgc cacgcggtaa atgttaattt
     4921 taatcgccag tacgctcggg tcgaacgaag cctgacgcag cagttccagt acatgctcaa
     4981 aggtatgata aggataatag agcagcacat cgcgctcacg aatggcatca aaaccgttgc
     5041 ggaactgggc tttatcaaac caaatgtggc gtaaacgtgg cagtggtttg ttcaccagat
     5101 tggctttgcc gacattgggg aaattaataa agtctttaaa attatgataa cgaccgccgg
     5161 ggacgatgga gtcgtagcgg gaaatagtca gtttttcgcg taacacttca accagcgcat
     5221 tgggcatatc gcgctgataa acaaaacgca ccggctcagc agttaaacgc tgcttgagac
     5281 tggaagacat caactccatc aggctggctt ccatctcatg cactaaatcg tattcggcat
     5341 cgcgggtcat cttcattgaa taggcattca gcgcgtcata atcaaagaag cctttgaaaa
     5401 tatcatcaag gcagtaacgc agaatgttat ccagaagaat catcggcttg cgtcgacgcg
     5461 gcgcttctgg cggtaaattc acaaagcgcg gcactttatc tgatgggatc tccagcagcg
     5521 cgtaacggat ggtatcgcca cggataattt ccaccgccag ataggtgtaa tcatctttca
     5581 ggaactgcac taaatcagtg tcaggattga ttaaaatcgg cgtaatgtgc tgacgcagat
     5641 actgcttaaa ataatgacgc agccagtttt gttgattgac ggagagctgg cgttcattaa
     5701 tcaggaagat ctggttgcgc gccatctcta gcaacaactc gttgtagagg ccgtcgaatt
     5761 cctgatcggc tttcagcacc cgggactgaa ttttgcccag taaatggcgg gaatgagagt
     5821 tggaaccttg ttcttcgcta ataatgatgc gtcgcttcag ttcagcgaag cggactttat
     5881 agaactcatc aaggttattg gaatagatcc ccaggaaacg catcctttca atcagcgggt
     5941 tagatttgtc cgccgcttcc tgaagcacgc gttcattgaa cgataaccaa ctgagctctt
     6001 tttcgatgta tagcttttcc tgacccatta ccacttttac tccgttttat tcacgggaca
     6061 ctgcctggat attatggcga gcaatattgg cagatgtcca actatgccag aaaaggggga
     6121 aaaaaactgc tggagcttgc gatttacagg tgaattgaaa aacgccagcg gcagagctgg
     6181 cgctttaact acgggggcat tactcgtcgg cagcgtagcc ctgcggcggc agacgttgac
     6241 catccagcca cgcggcgttt tcgtgcattt tcagacgacc atcggcaaac cagctaatca
     6301 ccagtggata aatagcgtgt tcctgggttt gcacgcgggc ggtgatgtca tcttccgtat
     6361 caccagcaaa taccgggact ttcgcctgta aaataaccgg gccaccgtcc agttcatcgg
     6421 tgacgaaatg caccgatgta ccgtgctctt catcgccatt ttccagcgcc tgacgatggg
     6481 tgtgtaatcc gggatatttc ggcagcagag aagggtgaat gttcagcaaa cgcccagcat
     6541 agtgggagac aaacgccggg ctgagaatgc gcataaaacc agccagcacg accacatcgg
     6601 gtgcgtacat gtcgatttca tgaatcaact cccggtcata ggcttcacga ctgtcaaacg
     6661 cgctggtgat gagcgtatgc gttgcaatac ccgcctggcg ggcgcgttca aggccgaacg
     6721 cgtcggcctt attgctgaaa actgcccgta cggtgccttt aattttgttg gttttgcagg
     6781 cgtcaataat tgcctgcaaa ttacttccgt tgccggaaat aagcaccaca atattcatta
     6841 ttcgataacc acgcgttgtt cggaatcaga ggctttgatg ataccgattt tccacgcgtt
     6901 ttcaccgttg gcattgagca gggcgagggc tttgtccact tccggagcag gcagggcaat
     6961 aatcataccg acgccgcagt tgaaggtgcg atacatttca tggcgctcaa cgttacctgc
     7021 cgtttgcagc cagttgaaca cttccggcca ctgccaggaa gattcatcaa tcactgcctg
     7081 ggtattatct ggcaatacgc gcggaatgtt ttcccagaag ccgccgccgg tcaggtgcgc
     7141 aatggcatgc acatcgacct tttcaatcaa tttcagcact gacttcacgt aaatgcgggt
     7201 cggtgccagc agatgatcgg ctaatggctt accatcaagt tcggtggttt gcggatcaca
     7261 accgctgact tcaagaattt tgcgcaccag cgaatagccg ttcgagtgcg ggccgctgga
     7321 gccgagtgca atcagcacat cgccgtcgct gactttagat ccgtcgatga tttctgattt
     7381 ttctaccacg ccaacgcaga aaccagcgac atcgtaatct tcaccgtgat acatccccgg
     7441 catttccgcc gtttcgccac ccaccagtga acagcccgat tgcagacaac cttccgcaat
     7501 gccgctgatc accgctgaag cggtatcaac atccagtttt ccggttgcgt aatagtcgag
     7561 gaaaaacagc ggttccgcac cttgcaccac caggtcatta acgcacatgg cgaccagatc
     7621 aataccaatg gtgtcgtgac gttttaagtc catcgccaga cgcagcttgg tacctacgcc
     7681 gtcagtgccg gaaaccagca caggttcacg atatttttgc ggcaatgcac acagcgcacc
     7741 gaagccgccc agaccgccca tcacttccgg acgacgcgtt ttcttcacta cgcctttgat
     7801 tcttccaacc agagcattac ccgcgtcaat atcaacaccg gcatctttgt agctaagaga
     7861 ggttttatcg gtcactgctt gggtccccac gcgttacttg cggtagaaaa ataaaattcg
     7921 gcgcaattct aacagggaaa gcaaacgttt gcgagactgc tttacacaac ctttttgcac
     7981 gtcttttccc caggcgcgcg gcgaaagaat acttgtacca gggcaaagga tcgttttcgg
     8041 atggaataat cttctttcat aactatttga atataaaata actttatctc aaaccgttat
     8101 cattttgact aaagtcaacg aaaataatat tgccgccttg aagaaaggag gtataatccg
     8161 tcgatttttt ttgtggctgc ccctcaaagg agaaagagta tgaagatcgt ggaagtcaaa
     8221 cacccactcg tcaaacacaa gctgggactg atgcgtgagc aagatatcag caccaagcgc
     8281 tttcgcgaac tcgcttccga agtgggtagc ctgctgactt acgaagcgac cgccgacctc
     8341 gaaacggaaa aagtaactat cgaaggctgg aacggcccgg tagaaatcga ccagatcaaa
     8401 ggtaagaaaa ttaccgttgt gccaattctg cgtgcgggtc tggggatgat ggacggtgtg
     8461 ctggaaaacg ttccgagcgc gcgcatcagc gtcgtcggta tgtaccgtaa tgaagaaacg
     8521 ctggagccgg taccgtactt ccagaaactg gtttctaaca tcgatgagcg tatggcgctg
     8581 atcgttgacc caatgctggc aaccggtggt tccgttatcg cgaccatcga cctgctgaaa
     8641 aaagcgggct gcagcagcat caaagttctg gtgctggtag ctgcaccaga aggtatcgct
     8701 gcgctggaaa aagcgcaccc ggacgtcgaa ctgtatactg catcgattga ccagggactg
     8761 aacgagcacg gatacattat tccgggcctc ggcgatgccg gtgacaaaat ctttggtacg
     8821 aaataaagaa taaaaataat taaagccgac tttaagagtc ggcttttttt tgagtaaagc
     8881 gcctataaca cataatacag aggataatac tatgacgcgc cgtgctatcg gggtgagtga
     8941 aagaccgcca cttttacaga caatcccgct tagtttgcaa catttgttcg ccatgtttgg
     9001 tgcaaccgtc ctggtgcccg tcttatttca tattaacccg gcgactgtac tgttatttaa
     9061 cggtatagga acactgctgt atctcttcat ctgtaaaggg aaaattccgg cttatcttgg
     9121 ttccagcttt gcctttattt caccggtatt gttactgttg ccgttggggt atgaagtcgc
     9181 gctgggcggc tttattatgt gcggcgtgct gttctgcctg gtttctttta tcgtgaagaa
     9241 agcggggacc ggctggctgg acgtgctgtt tccacctgcg gcaatgggcg caatcgttgc
     9301 cgtcatcggt ctggagctgg cgggcgtagc tgccggtatg gcgggtttac tcccggctga
     9361 agggcaaacg ccagactcca aaaccatcat catctctatt accaccctgg cggtcacggt
     9421 tttaggttcc gtgctgtttc gtggtttcct ggcaattatc ccgattttaa ttggcgtgct
     9481 ggtggggtac gcgctctctt tcgcaatggg aattgtcgat accacgccga ttattaatgc
     9541 tcactggttt gcgctgccga ccctctatac gccgcgcttc gagtggtttg ccattctgac
     9601 tattctgcca gcggcgttag tggttattgc cgaacacgta gggcacctgg tagtaacggc
     9661 taatatcgtc aaaaaagatc tgctgcgcga tccaggtctg caccgttcga tgtttgctaa
     9721 cggcttgtcg accgtgatct ccggcttctt tggctccacg ccaaacacca cttacggaga
     9781 aaacattggc gtgatggcga tcacccgtgt ttacagtacc tgggttatcg gcggtgcggc
     9841 gattttcgct atcctgcttt cctgcgtcgg taaactggct gctgctatcc agatgattcc
     9901 attgccggtg atgggcggcg tttcgctgct gctttacggt gtcatcggtg cttccggtat
     9961 tcgtgtgttg atcgaatcga aagtggatta caacaaagca cagaacctga tcctgacttc
    10021 cgtgattttg atcatcggcg tcagtggcgc gaaggtaaac attggggcgg cggagctgaa
    10081 aggtatggcg ctggcgacca tcgtcggtat cggcttaagt ttgatcttca aactgattag
    10141 tatgctgcgc cctgaagaag tggtgctgga cgcagaagac gcggatataa cagacaaata
    10201 atgtaccgac cgggcagtgt tgctgcccgg ttcaaacatc atgggattct gtggtaaact
    10261 tctcgcgatt ttgtgaaatc ctggttgagg tatctctgaa cacaccggca cagctctctt
    10321 tgccacttta tcttcctgac gacgaaacct ttgcaagttt ctggccgggg gataactcct
    10381 ctttactggc cgcgctgcaa aacgtgctgc gtcaggaaca tagcggttac atctatctct
    10441 gggcacgcga aggcgcgggg cgcagccatc tgctgcacgc ggcttgcgcg gaattgtcgc
    10501 agcgtggcga tgcggtgggc tatgtcccgc tggataaacg cacctggttt gttccggaag
    10561 tgctcgacgg tatggagcat ttgtcgctgg tctgtattga caacattgag tgtattgccg
    10621 gcgatgagtt gtgggagatg gcgattttcg atctctacaa tcgaattctg gaatcgggca
    10681 aaacacggtt gttgatcacc ggcgatcgtc caccgcggca gttgaatctg ggattaccgg
    10741 atctcgcgtc gcgtctcgac tgggggcaga tctacaaatt gcagccactt tctgatgaag
    10801 ataagttgca ggcgctacag ttacgcgcgc gtttgcgtgg ttttgaactg ccggaagatg
    10861 tggggcgttt cttgctgaag cggctcgaca gagaaatgcg cacgttgttt atgacgctgg
    10921 atcagctgga tcgtgcgtcg attaccgcgc aacgcaagct gaccattcct tttgtgaaag
    10981 aaattctgaa gttgtagata aggtgtttat tgtcggatgc ggcgcgaacg ccttatccga
    11041 cctacgcgaa gttaatccat agtattcgta ggccggataa gacgcggcaa gcgtcgcatc
    11101 cggcaatgtg cttcaactag cgatggagca catcttaacc cacgatttcc agtacctgct
    11161 ccggtggacg accaatccgt gctttgccat tcgcgaccac aatcgggcgt tccattagtt
    11221 tcggattatc caccatcgcc tgaatcagcg cctcttcgct aagtgaactg tcagccaggt
    11281 tcagttcttt ataaagatcc tctttttggc gcatcaattc acgagcgcta ttcatcccca
    11341 gcattttcag caaatcacgc agcgttgccg catcggcggg tgtctcaagg tagagcacca
    11401 cttctggctc cacgccgttt tctttcagca ggttcagcgt ttcccggctc tttgaacagc
    11461 gtgggttatg gtagattttt acctgtttgg tcattgttct tcctttaatg cgaattacat
    11521 cttggtataa ggcttaaagc gttcctgcag ctggcgcaac tggtcgatgc gcgcatcgta
    11581 acgcgcttgt tgcaggctgc ctaatttcac ctgcgaactg gcgctactca acaaggaaat
    11641 agcctgatcg agtcgcccgg cgagcgcata accttctgct cgcgcagcca gttcctgatc
    11701 gcggttattt agtgcggctt ccgcctgtgc cagtaaatcc cagccgttgc tgtcatcttt
    11761 attgttaaaa gtgtagcgat tcagaatatt ggccgcttcc tgtggttgcc ccccttgcag
    11821 ataagcgttc gccaggttga gctgcaacac cggattggtg cgcaaatcgc gggcattttt
    11881 caggcgattg atagcgtcat tggctttgtt ttgcccaaga tcgatatcgg tagccagatc
    11941 gagataccat gcgttgccag gttctgctgc cagtaacggt tgcagcgttt tacgcgcttc
    12001 gtcgtattta ttggcttcca tcgcctgtag agcacgacca tattgcgccg ctcgttgctg
    12061 acgaacgttt cctttcgccc attcatccag caaatcactg gtgagctggt tacgtccgga
    12121 attatacatc cccagtgtgc gcgctttcgc cagatagaaa tcttccgacg actgcaccac
    12181 catcgggcgc atctgattag cacggttgcg ggcatctgcc agacgacttt ccggcaacgg
    12241 gtgagtcaat aaaatttccg gcggacgcga ggagtaacgc gcctgatcga gtaatttttc
    12301 gaggaaggtc ggcatcgcct gcggatcgaa tcccgagcgt tgcagcacct gaataccaat
    12361 gcggtccgct tcctgttcat tttgctgggt gaaactgatc atcccctggc gcgttcccgc
    12421 cagtgtaccg gtcagcgccg ccatccccgc ctgcggactg gccatcgcca gtaaaataga
    12481 acctaacgcg ccgacccagg tcagcggcgc gctgcgctgc tgatcttcca tcgctcgcgc
    12541 caggtgacgt tgggtgacgt gggagatttc gtgcgccata actgaagcca gttgactttc
    12601 gttatcggaa taacggaaca gggcagagtg cagcaccacg ttgccgccaa agaaagcaaa
    12661 ggcgttaatt tcgtcgttgt tgatcagaaa gaaatgaaac ggtgtcttaa ccgaattggc
    12721 atgcgaaacc agacgcattc ctagcgaatt aatatattgc gttaatagcg ggtcattaat
    12781 taacggcgcg ctgccgcgta actggcggac ataatagtcg cccatctgca tttcctgacc
    12841 aatggaaagt gtgcttcctg cggaggttcc catatccggc aaggtgtctg cgctgtcggc
    12901 aaacgccggg gctacctgac caatggtcat agcagcaatg agggttgcaa ccaggttttt
    12961 tttcaactgc ctgaacataa cctctatcct gtatttctgg agatagtcat ttgaccgata
    13021 ctgtgaaata tcgttcccac cgcgccgcct ttgagtgtaa cgacgaagat tagcccactc
    13081 aaataacttt tcagtttcct gaatccgtca taaaaagcga agtctttttt gtgacatttc
    13141 gatacaattg cggatcgcaa tcccgctctt gaggttcagg taaggtctgt atgctcgaaa
    13201 tgttgatgca atggtatcgc cgccgtttta gcgacccgga agcgattgcc ttgctggtta
    13261 ttttagtcgc cggatttggc attatctttt tctttagtgg cctgcttgct ccgttgctgg
    13321 tggctattgt gctggcctat ttgctggagt ggccaaccgt gcgcctgcaa tctattggct
    13381 gttcccgccg ctgggcgacg tcgattgtat tggtggtttt cgtcggtata ttgctactga
    13441 tggcgttcgt ggttctgcct attgcctggc agcagggcat ctacttaatc cgcgatatgc
    13501 cggggatgct caataagctt tctgactttg ccgccacgtt gccgcgccgc tatccggcgt
    13561 taatggatgc gggcattatt gatgcaatgg ccgaaaatat gcgcagtcgg atgctgacca
    13621 tgggcgattc ggtggtgaaa atttccctcg cctcgttggt cggtttgctg accattgccg
    13681 tctatctggt gctggtgcca ttgatggtct tcttcctgct gaaagacaaa gagcagatgc
    13741 tgaacgccgt tcgccgggtg ctgccgcgca accgtggact ggcaggacag gtgtggaagg
    13801 agatgaatca acaaatcacc aactatatcc gcggcaaagt gctggagatg atcgtggtag
    13861 ggatcgccac ttggctgggg ttcttgctct ttgggctgaa ctattcgctg ctgctggcgg
    13921 tgctggtcgg cttctcggta cttattccgt acattggcgc atttgtggtg accattccgg
    13981 tggttggcgt ggcgctattc cagtttggtg ctggcacgga attctggagc tgcttcgcgg
    14041 tgtatctgat tattcaggcg ctggacggca acctgttagt accggtgttg ttctccgaag
    14101 cggttaacct gcatcctctg gtgattattt tatcggtggt gatcttcggt ggtttgtggg
    14161 gattctgggg cgtattcttc gctattccat tggcgacgct gatcaaagcc gtgattcacg
    14221 cctggcctga tgggcaaatc gcgcaagact gaccttcagc gaaaaatttg aaacgagtgt
    14281 tgcagggcag tgagaagatt tgagatgtgc tggccgcaag cgcagccagc acggaatgga
    14341 gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt gattgctggt
    14401 tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga agctgatgcg
    14461 atgaatacca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc cgaattgttc
    14521 gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt tttccgcaaa
    14581 acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc ccgctttttt
    14641 caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg gggtcatggc
    14701 tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat taacttgttc
    14761 tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca gtggattcat
    14821 tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat actgccttgt
    14881 gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc gtccgcagat
    14941 gcggggctgt gggcagttat ctgaatatgt aactgcgcag cccgttcatt ttcagcaggt
    15001 tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag tgctgtgaag
    15061 cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga tgctggcatt
    15121 ggtggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag ttcggcacct
    15181 ttcaacggta acgttgattc aatcagggtg attgcattcc atgaaccgga aagcagcatg
    15241 ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt acagccgcaa
    15301 ctactgacat gacgggtgat ggtgttcact attccagggc gatcggcacc caacgcagtg
    15361 atcaccagat aatgttgcga tgacagtgtc aaactggtta ttccttcaag gggtgagttg
    15421 ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc tgtctgcttg
    15481 cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg atggcccatg
    15541 ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa tgtctgtcgg
    15601 gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc gatcgtttct
    15661 gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga tgtggtgatg
    15721 atgacgctgg agctggctga cgggcgcatt ccggtgattg ccgggaccgg tgctaacgct
    15781 actgcggaag ccattagcct gacgcagcgc ttcaatgaca gcggtatcgt cggctgcctg
    15841 acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca tttcaaagcc
    15901 atcgctgaac atactgacct gccgcaaatt ctgtataatg tgccgtcccg tactggctgc
    15961 gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat cggaatcaaa
    16021 gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc agatgatttt
    16081 gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg cggtcatggg
    16141 gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg caaactggca
    16201 gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc attacacaac
    16261 aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga actgggtctt
    16321 gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg tcgtgagacg
    16381 gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt tgatggctta
    16441 ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt tattactcgc
    16501 tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag cctacctgga
    16561 agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg tgacctccgg
    16621 cgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc tggacattcg
    16681 tccaccagcc cagccgttgg cactggtttc tggcgcgcgt acccagttca cgggcgatac
    16741 cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg tggttagcgt
    16801 gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga cactgaccac
    16861 cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc gttatcaaat
    16921 ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgctga acctggaaca
    16981 ggcgggcaaa ccggttgcag acgcggcgtc catgcagcgt tacagcacgg agatgatgaa
    17041 cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg cgcaaaatcg
    17101 tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac caatgctggt
    17161 cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gctgcgctgg aaaaagtggg
    17221 catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt ataagccgct
    17281 gtctgacagc gaatggcacg aactgggcgc gagcgatcca ggtctggcat ccggtgacta
    17341 taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg atccgaaagg
    17401 tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg ctgcgtttag
    17461 caagtaaaaa tacagggctg gagtcatccg gccctttttt ctgatatgat acgcaaacgt
    17521 gtgcgtcggc agaaaaacgc gattttagcg gtaattcgca cgaaatttgt ttgtcggacg
    17581 taggtcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc agacagcaaa
    17641 agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca agctgagttg
    17701 tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt ggtgctcgaa
    17761 ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga tcgcaaaggt
    17821 atggtgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc gggtatcccg
    17881 actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct ggatatggtg
    17941 ccggttgagt gtgtcgtgcg taaccgtgct gctggctctc tggtgaaacg tcttggaatc
    18001 gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa cgacgccatg
    18061 cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag caaagagaac
    18121 ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa actgttcgat
    18181 gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa aggcgaagtg
    18241 gttctgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga aacgctggag
    18301 aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc ctatgaagcc
    18361 gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc ttgccgtgct
    18421 gctggcacgg caagacaacc gctctcataa gatgagtatt gaacgctcat tcctccgcca
    18481 tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat ttctataatg
    18541 aatataatgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac aatgttgaag
    18601 acaggcgcaa tagctccggc ggtccgtcaa tgggcggtcc cggttttcgc ctgccaagcg
    18661 gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat ggcgttgatt
    18721 taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacgcgg tcaattagcc
    18781 ctaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg gaagacacct
    18841 ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg gtcatgtacc
    18901 gtggaatgac gcgtaccggc tgcggggcgg gccagtccat aatggggcca ttctattgcc
    18961 cggcggatgg cacagtttat atcgatctct ccttctatga tgacatgaaa gacaaacttg
    19021 gcgcggatgg cgattttgcc caggggtacg ttatcgctca tgaagtcggt catcatgtgc
    19081 agaaactgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg acgcaggcgg
    19141 aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc ggtgtctggg
    19201 ggcatagtat gcagcagcaa ggtgttctgg aaaccggcga tctggaagag gcgctgaacg
    19261 cggcgcaggc catcggcgat gaccgtttac aacagcaaag tcaggggcga gtagtaccag
    19321 acagtttcac tcatggcact tctcagcaac gctacagctg gtttaaacgt ggtttcgaca
    19381 gcggcgatcc ggcacaatgc aatacttttg gtaaaagcat ttaacttccg gggcagggat
    19441 ggctgaactg actgcgcttc acacattaac agcgcaaatg aaacgtgaag ggatccgccg
    19501 cttgctggtg ttgagcgggg aagagcgttg gtgttttgat catgcgctta agttgcgtga
    19561 tgccttacct ggcgactggc tgtggatttc gccgcagcca gatgctgaaa accactgttc
    19621 tccctcggca ctacaaactt tacttgggcg cgagttccgg catgcggtat tcgacgcccg
    19681 ccacggcttt gatgccgctg cctttgcggc acttagcgga acgttgaaag cgggaagctg
    19741 gctggtgttg ttactccctg tatgggatga gtgggaaaac caacctgatg ccgactcgct
    19801 gcgctggagt gattgccctg accctattgc gacgccgcat tttgtccagc atttcaaacg
    19861 cgtacttacg gcgaataacg acgctatcct ctggcggcaa aaccagccgt tctcgttggc
    19921 gcattttact ccccgtactg actggcaccc cgctaccggc gcaccacaac cagaacaaca
    19981 gcaactctta cagcagctac tgaccatgcc gccgggtgtg gcagcggtaa ctgctgcgcg
    20041 tgggcgcggt aagtcggcgt tggcagggca actcatttct cgtattgcgg gcagtgcgat
    20101 tgtcaccgct cccgcaaaag cggcaacgga tgtactggca caatttgcgg gcgagaagtt
    20161 tcgctttatt gcgccggatg ccttgttagc cagcgatgag caagccgact ggctggtggt
    20221 cgatgaagcc gcagctatac ctgcgccgtt gttgtatcaa ctggtatcgc gttttcctcg
    20281 aacgttgtta actactacgg tgcagggcta cgaaggcacc ggacgtggtt ttttgctgaa
    20341 attttgcgct cgctttccgc atttacaccg ttttgaacta caacagccga tccgttgggc
    20401 gcagggatgc ccgctggaaa aaatggttag tgaggcactg gtgtttgacg atgaaaactt
    20461 cacccataca ccacaaggca atatcgttat ttccgcattt gaacagacgt tatggcgaag
    20521 cgagccagaa acgccgttaa aggtttatca gttattgtct ggtgcgcact accggacctc
    20581 gccgctggat ttacgccgca tgatggatgc accaggtcaa cattttttac aggcggctgg
    20641 cggaaacgag attgccggag cgctgtggct ggtggatgag ggcggattat ctcaagaact
    20701 cagtcaggcg gtatgggcgg gttttcgtcg cccgcggggt aatctggtgg cccagtcgct
    20761 ggcggcgcac ggcagcaatc cactggcggc gacattgcgc ggacggcggg ttagccggat
    20821 agcagttcat ccgactcgtc agcgggaagg cacagggcga cagcttattg ctggtgcttt
    20881 gcaatatatt catgacctcg actatctttc ggtgagtttt ggttacaccg aggagttatg
    20941 gcgtttctgg caacgctgcg gttttgtgct ggtgcgaatg ggtaatcatc gtgaagccag
    21001 cagcggttgc tatacggcga tggcgctgtt accgatgagt gatgcgggta aacagctggc
    21061 tgaacgtgag cattaccgtt tacgtcgcga tgcgcaagct ctcgcgcagt ggaatggcga
    21121 aatgcttcct gttgatccac taaacgatgc cgtcctttct gacgacgact ggcttgaact
    21181 ggccggtttt gctttcactc atcgtccgct attaacgtcg ttaggttgct tattgcgtct
    21241 gctacaaacc agcgaactgg cattaccggc gctgcgtggg cgtttacaga aaaacgccag
    21301 tgacgcgcag ttatgtacca cacttaaact ttcaggccgt aagctgttac tggttcgcca
    21361 gcgggaagag gccgcgcagg cgctattcgc acttgatgat gttcgcactg agcgtctgcg
    21421 cgatcgcata acgcaatggc aattttttca ctgactcctt caattttctc ccatggtcat
    21481 tgtgagtcag cggcgtaaaa ttaatgacat cagttaagga gttcgccatg aaacatgacc
    21541 attttgttgt tcaaagcccg gataaaccag cacaacagtt gttactgctt ttccacggtg
    21601 tcggggataa cccggtggca atgggggaaa taggtagctg gtttgcgccg ctgtttcctg
    21661 atgcgctggt ggtgagtgtc ggtggcgcgg agccgagcgg taatccggcg gggcgtcagt
    21721 ggttttcggt gcagggtatt acggaagata atcgccaggc gcgtgtgaat gccatcatgc
    21781 cgacgtttat tgagacggtg cgctactggc agaaacaaag cggagtaggg gctaacgcca
    21841 cggcgctaat cggtttctca caaggcgcga ttatggcgct ggagagcatt aaagccgaac
    21901 cgggccttgc ttcgcgcgtc attgccttca acggacgcta cgcgagcctg ccggaaaccg
    21961 cgtctacagc cacaacaatt cacttgattc atggcggtga agatccggtt atcgatctgg
    22021 cgcatgcggt tgcggcgcag gaagcgttaa tcagtgccgg tggtgacgtg acgctggata
    22081 tcgtggagga tttaggccat gcgatcgaca atcgcagtat gcaatttgcc ctggatcact
    22141 tgcgttatac cattccgaaa cattatttcg acgaagcgct aagcggcggt aagcctggcg
    22201 atgatgatgt gattgaaatg atgtaataat agccgggtag gtctgataag actgtatcag
    22261 gcatcatggc cggatgcggc gttaacgcct tatccggcca cttagcttac ttcttcggtt
    22321 gatccttttt cggccagtcg tcgtcatcgt cccacttatc gttgaaatca cgatgcgggg
    22381 gaagctccgg cttgttggcg agaaatttct tatggtcgac gcgcttgaga tctttaatca
    22441 cgttcagcag gacgcctacc aaaaagacaa tcaccagaat ccaccaatat ttagccagcc
    22501 agtccatgct tatttcctct tgcagaacca ctcatcaggc gacgagctgt tccatgatac
    22561 gttgatacat acgggcaagt agctgcaggt cggcagcgtt cacacattca ttgattttat
    22621 gaatcgtggc attgaccggc ccgagttcca ccacctgcgc ccccatgcgg gcaataaagc
    22681 gcccgtcgga cgttccgcct gtggtcagta gctgcggttt aatttcatta tagtgctcaa
    22741 ccgcgttaac gaccgcatcc accagtttac cgcgcgcggt caaaaatggc tgcccggaaa
    22801 gccaccaatc caccgtatag cgcagttgat gtttttcaag cagggcaagc acctgcgctt
    22861 tgatcatctc atccgtcagt tcggtgctaa agcggaagtt aaactgcaca aacagttcac
    22921 ctggaataac gttgttactg cccgttcccg cctgaatatt ggcaatctgc atactggtcg
    22981 ccgggaagaa gtcattaccc tgatcccact caatagccac taattcatta atgaaaggtg
    23041 ctgcgcgatg taccggattg tcagccagat gtgggtaggc aacatgcccc tgaacgccat
    23101 gaatggtaag gttgcaggtt aacgatccgc gacgaccatt tttcaccaca tcacctacca
    23161 cttcgatact cgacggttcg ccaaccagac agtaatcgag acgctcatta cgcgccatta
    23221 acgcttcgac gacttttacc gtaccgttgt gggcactagc ttcttcatca gaggtgatca
    23281 gaaatgccag tcgccctgta tggttgggat gttgtgcgac aaagcgttct gccgctacca
    23341 ccatcgccgc cagcgagcct ttcatatctg ccgcacctcg cccaaataac atgccgtcac
    23401 gaatggttgg ttcaaatggt ggattgatcc aacggtcggc gtcgccaggc ggcaccacgt
    23461 cggtatgccc ggcaaaggct aacgtttcac cctgcccgcg ccatgcccaa aaattctgcg
    23521 tatcggcaaa gtccatgcgt tcaacggtaa aaccgatcgc ctgcaaacgt tcaatcaaca
    23581 aagcctggca tcctgcgtca tcaggactca gggaagggcg gcgaataagc tgttgtgtca
    23641 gctcaataac cgggcacgac atagactaca cctcatggaa aaattgctga taactggatt
    23701 cactgaaacc cagcagcata ggcttaccag gcgcgcagag caatggacgt ttgataattg
    23761 caggcatttc agtcattaat gtcgccgcag aggccgcatc ggtgatttta ttgcgggtgg
    23821 tttcgtccag tttacgccag gttgtaccac gggtgttgag taacgcttcc cagcctaatt
    23881 cgttgataaa accgttcaat aattcgctgt ccaatccatc gacgcggtaa tcatgaaaac
    23941 gatagtcgat gttattggct tccagccaac ggcgagcctt tttaatggtg tcacaatttt
    24001 tgatgccgta aagtgtaacc ataatgaatc ctttttcgat ttatgagaat aaatactcat
    24061 tt
//
LOCUS       DACADF010000071        23683 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.071, whole genome shotgun sequence.
ACCESSION   DACADF010000071 DACADF010000000
VERSION     DACADF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.071"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(197..1087)
                     /gene="metF"
                     /locus_tag="ISB30_002648"
     CDS             complement(197..1087)
                     /gene="metF"
                     /locus_tag="ISB30_002648"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAP1990264.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRII
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(1469..3022)
                     /locus_tag="ISB30_002649"
     CDS             complement(1469..3022)
                     /locus_tag="ISB30_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP1990265.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELMGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSNRPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3155..4285
                     /locus_tag="ISB30_002650"
     CDS             3155..4285
                     /locus_tag="ISB30_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAP1990266.1"
                     /translation="MSTLFTINACKSFGCRNLGQPTSTDYSWPDYRLGYPALHCRACG
                     SYPPLFDEQQFRDWLTVHLSTFATEKGHFCPVCYGTETICYGHNPQGSQRIQCRNCKK
                     VWTPKQYQKEITTPEIIETVALLVPFQGVSSGQKLYVLISFDALRGNILHLSTNYTQH
                     QAGESLHYRYRGNAEPELHDNNIVQRVDMREAQFLRRSQFDEIQYGSAALKRNAKGAI
                     LRPVITAHGHFRVLNILFPTVKTHVISHECFLRGAIITAWADLFRQQQGEIWFIEEEI
                     ADDTDNMPWRFQGKTYHGWWKNQWQLWVQGKNRKMVCALTGGKSSKAEMLSLATSRHF
                     IDWLHKQAVFTHSAPLSAGRVTQILLSLAQDYNNCASRLISD"
     gene            complement(4249..6681)
                     /locus_tag="ISB30_002651"
     CDS             complement(4249..6681)
                     /locus_tag="ISB30_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAP1990267.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADNLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPSAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6684..7844)
                     /gene="metB"
                     /locus_tag="ISB30_002652"
     CDS             complement(6684..7844)
                     /gene="metB"
                     /locus_tag="ISB30_002652"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP1990268.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            8121..8438
                     /gene="metJ"
                     /locus_tag="ISB30_002653"
     CDS             8121..8438
                     /gene="metJ"
                     /locus_tag="ISB30_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAP1990269.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            8498..9106
                     /locus_tag="ISB30_002654"
     CDS             8498..9106
                     /locus_tag="ISB30_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAP1990270.1"
                     /translation="MKIRLLIPGLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEDSKYIVRRVKGELNAEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(9167..9379)
                     /gene="rpmE"
                     /locus_tag="ISB30_002655"
     CDS             complement(9167..9379)
                     /gene="rpmE"
                     /locus_tag="ISB30_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAP1990271.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            9582..11780
                     /gene="priA"
                     /locus_tag="ISB30_002656"
     CDS             9582..11780
                     /gene="priA"
                     /locus_tag="ISB30_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP1990272.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDDFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILASPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            11936..12961
                     /gene="cytR"
                     /locus_tag="ISB30_002657"
     CDS             11936..12961
                     /gene="cytR"
                     /locus_tag="ISB30_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAP1990273.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSRAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            13053..14012
                     /gene="ftsN"
                     /locus_tag="ISB30_002658"
     CDS             13053..14012
                     /gene="ftsN"
                     /locus_tag="ISB30_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAP1990274.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            14105..14635
                     /gene="hslV"
                     /locus_tag="ISB30_002659"
     CDS             14105..14635
                     /gene="hslV"
                     /locus_tag="ISB30_002659"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAP1990275.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            14645..15976
                     /gene="hslU"
                     /locus_tag="ISB30_002660"
     CDS             14645..15976
                     /gene="hslU"
                     /locus_tag="ISB30_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAP1990276.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            16043..16969
                     /gene="menA"
                     /locus_tag="ISB30_002661"
     CDS             16043..16969
                     /gene="menA"
                     /locus_tag="ISB30_002661"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP1990277.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            17062..17547
                     /gene="rraA"
                     /locus_tag="ISB30_002662"
     CDS             17062..17547
                     /gene="rraA"
                     /locus_tag="ISB30_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAP1990278.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(17632..17877)
                     /gene="zapB"
                     /locus_tag="ISB30_002663"
     CDS             complement(17632..17877)
                     /gene="zapB"
                     /locus_tag="ISB30_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAP1990279.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            18302..19147
                     /gene="glpF"
                     /locus_tag="ISB30_002664"
     CDS             18302..19147
                     /gene="glpF"
                     /locus_tag="ISB30_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HAP1990280.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            19170..20678
                     /gene="glpK"
                     /locus_tag="ISB30_002665"
     CDS             19170..20678
                     /gene="glpK"
                     /locus_tag="ISB30_002665"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP1990281.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGMEEYIRNNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            20849..21859
                     /gene="glpX"
                     /locus_tag="ISB30_002666"
     CDS             20849..21859
                     /gene="glpX"
                     /locus_tag="ISB30_002666"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAP1990282.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            21956..22702
                     /gene="fpr"
                     /locus_tag="ISB30_002667"
     CDS             21956..22702
                     /gene="fpr"
                     /locus_tag="ISB30_002667"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAP1990283.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(22707..23135)
                     /gene="uspD"
                     /locus_tag="ISB30_002668"
     CDS             complement(22707..23135)
                     /gene="uspD"
                     /locus_tag="ISB30_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAP1990284.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(23162..23461)
                     /locus_tag="ISB30_002669"
     CDS             complement(23162..23461)
                     /locus_tag="ISB30_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAP1990285.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
BASE COUNT         5752 a         6160 c         6170 g         5601 t
ORIGIN      
        1 cgtaattaag actttttgat tatgtccgat ttaggacaaa tgctttataa aaaagggttt
       61 tagatttgta tccttcaagt aatgagggag aaaacaaggc tgataacctt attttcccgc
      121 cctcatttcg aggcagcatt ttgtgctctg tttaaaattt gtgaacactg tgtgattttc
      181 acaaaaggca caatacttat aaaccaggtc gaactcccag tgtatggcaa atcgcataac
      241 tcatttcagc gcggttaagc gtatagaagt ggaaatcttt cactccttca cggcttaaaa
      301 tcttcaccat atccatggcg atattcgcgc ccaccagctt gcgggtttca gcatcgtcat
      361 ccaggccgtc gaacatttgt gccatccacg ccggaatacg cacgttggtc atgtcggcaa
      421 atttcttcgc ctgtttaaag ttcgaaaccg gcaaaattcc cggaatgatt tccacatcaa
      481 tgcctgccga gacgcagcga tcgcgaaaac gcaggtagct ttcgacatcg aagaagaact
      541 gagtaatggc gcggttggct ccggcatcca cttttcgtct caggttgagt aaatcggcct
      601 gggcactttt cgcttctgga tgtacttccg gatacgccgc cacggagata tcgaaatctg
      661 ccacttcttt taacagcgtc accaggtcag aagcatacat ttctggctta ccacttcccg
      721 gcggcaggtc gccacgcagc gccacgatat ggcgaatacc gttattccag tagtcgcgtg
      781 caatgatccg cagctcggca ggcgtcgcat caatgcaggt aagatgcggt gccgcttcca
      841 ggccggtgcg atctttaatg cctttaataa tgctgtgcgt acggtcgcgc tcgccggagt
      901 ttgcgccata ggtcaccgat acaaacttcg gtttcaggct gctaaggcga tcgatggagt
      961 tccacagggt ctgctccatt tcactggtac gcggcgggaa aaattcgaac gaaacgttaa
     1021 tctgcccctg gacttctgcc agactctgat tcagggcatc ccgctggctg gcgtgaaaaa
     1081 agctcatacc ttacctcatc aatcgcttat cgttatatgt tgtgttgtgc acatctatcc
     1141 gtttagacgt ccagatgtaa agatgaagga aaagccgaag ggcgtcaacc gaaaaataac
     1201 caatgatggt gaggaaaaat cagggaagat gaaaaaaact catcttaatt agaggcgata
     1261 aaagatttct ctatagcgca atccacctcg cacgttttga ctcatattct gacaacacag
     1321 cgacacctgg gctaattcga aaaacaacgt cctgccaccg gatggtgatg ttaaggcatc
     1381 tgattcggcc tgcgccctcg cgtaggccga atacaggatc tgcgcaatca acgggtaaca
     1441 aacagttgag tcaattcagt gcgcactttt atttgatatc cttcacgcgc ataccgttga
     1501 gctgttcatc cgtaatggtg ttgcccgctt tgaagtaatc aaccaccgcg tgataaacgt
     1561 aataaccgcc cgttgtgtta cgcgctttcc cttcggtaaa ggcggtaaaa ccatctccac
     1621 catcagcaag gaaactctga gtagcaatgt gataaaccgt cgcatcttca atgggtttgc
     1681 cattcaaagt cagtgtgatt acccgctgac caaccggcct gttgctgtcg tacttcattt
     1741 ccaggccttt cgatacctgt aaaacgccat tgcttaaact tgcgccgtgt tccatcagac
     1801 tgcgtaattg tttacccatg agttccatcg tcaccagttc gttggggaac ggaaacgtac
     1861 tgatgacgcc gcccatcgtg atcgcccctg cggggatctc attgcggata ccaccagagt
     1921 ttgttaacgc cagttgggtg tttttacccg ccgctaccaa taaagcgtca gccgccaggt
     1981 tcccgaggga agccgattca ccataggcac gttttaattc aaccggcgat tgcgccaccg
     2041 tttgttgcac cacttcatca agctttttat tccagccgtc gatcacctgt ttcgtttgcg
     2101 gatcgggctt ccactcgtca gcgtaaatgg ttttaagctc gaagtttttc accgtaaaat
     2161 tatgcggctt ctctttgtag tcgagaacca gtttgcccac atcaatcccg ccgctgtcag
     2221 tggagaggat gagcgtattg ccgactttaa tcggttccgg cgtacccaca tgtgcatgtc
     2281 cggtgatcaa aatatccagc cctttcacct gacttgccgt ctgaatatct ttatccaacg
     2341 cgcgacgcac atctgtgcct cccatactgg actggcgggc tggaacacct tcgtggatca
     2401 gggcgacggt caaatcaacc ttgcctttga gttcatcgat ataacgttgt agccatttaa
     2461 tttcatcgcg cgcttcaata cccacgcgcg tggcagcaga taccgtatca ttaaaggcaa
     2521 atactccgtg caaaccaata acgcctattt tcacaccgtc tttttcgatg atggtatagg
     2581 gcttatccca gaatgattta ctgctgttct gataaaaaat attgccctgc acaataggga
     2641 attttgcctg gctcaactgt aataatgtgt tgtcccagcc gtggtcaaat tcatggttgc
     2701 ctattgtgac ggcatcaaac ggcatagtat tcataatatc gataatagcc ttacctttgg
     2761 tcaggctgct gatatatggc ccggtaaaat agtcgcccgc atcaaaaaac catgtcgctt
     2821 tattttttgc tttttcttgc ttaaccagcg tagtgatatt tgcccagccg ccaatatccc
     2881 gtttaccgtc agcgatccac ggcactttat aaggctcgac atgtgcatgg aggtcgttgg
     2941 tataaatgat ggtgacatct ttagcgcaag cccagaatgg cagtgttaat gcgatacccg
     3001 cagcaagtat tttaattttc ataaacagtc cctttcagtg agataaagga ttgctacatt
     3061 acgttctgaa agacttattt atgtgagagt aatcacatcc gcaggggata gttgccggac
     3121 aaaatcaacg accttgtgaa atcgttggtg atatatgtcg actctgttta cgattaatgc
     3181 ctgtaagtcg tttggttgcc gtaatttagg gcagccaaca tcgacagatt atagctggcc
     3241 cgactatcga ctgggttatc cggcactgca ctgtcgggca tgtggcagtt acccgccgtt
     3301 gtttgacgag caacaatttc gtgactggtt gacagttcac ttatctactt ttgcaacaga
     3361 gaaaggccat ttttgcccgg tatgttatgg cacagagacg atttgttatg gtcacaatcc
     3421 acagggttcg caacgcattc aatgccgtaa ctgcaaaaaa gtatggacac ccaaacaata
     3481 tcagaaagaa ataacgactc ccgaaataat cgaaacggtt gctttgctcg tgccatttca
     3541 gggagtcagc agcgggcaga aactgtatgt tttaatcagt tttgatgctc ttcgcggtaa
     3601 tattcttcat ctttctacta attacacgca gcatcaggct ggagaaagtc tgcattatcg
     3661 atacagagga aatgcagaac cagagttaca cgacaataat attgtacaac gcgttgatat
     3721 gcgcgaagca caatttctac gccgcagtca gtttgatgaa attcagtatg gcagcgcagc
     3781 gctcaagcgc aatgccaaag gcgctattct gcgtccggtt atcactgcac acgggcattt
     3841 cagagtactg aatattttat tccctacagt aaaaacacat gtaatttcac acgagtgttt
     3901 tttaagaggt gccattatta cggcctgggc tgatctgttt cgccaacagc aaggtgaaat
     3961 ttggtttatt gaagaggaaa tagctgacga taccgataat atgccctggc gttttcaggg
     4021 aaaaacttat cacggctggt ggaaaaatca gtggcaactc tgggttcagg gaaaaaatcg
     4081 taaaatggta tgtgcattaa cgggcggaaa gagtagcaaa gcagaaatgc tctctttagc
     4141 aaccagcaga cattttatcg actggctaca taaacaagca gtatttactc acagcgcccc
     4201 gctttccgca gggcgcgtta cgcaaatact gctttctctg gctcaggatt acaacaactg
     4261 cgccagtcgg ttaatatccg actgaatcgc cccggcggtg acgtcgcgcc ctgcacctgg
     4321 cccgcggatc accagcgggt tgtcgcgata ccagcggctt tcgatggcaa agacgttatc
     4381 gcatggcagt agtgatgcca gcggatggtc ttcacgcacc gcttccacgc ccacacgcgc
     4441 tttgccattg gcatcgaaac gcgccacgta gcgcagcacc agccccattt cgcgggccgc
     4501 ttccagtcgt tgcaccatct gctcgttaag ttcatcgcca ttttcaaaga aatggtcgat
     4561 gctgccgcct tcgcaatggg caggcaccag tgattcaaca cgtacctggt ccggttcaat
     4621 gttgtaaccc gcttcacgcg ccagaatcac cagcttgcgc attacgtctt tgccggaaag
     4681 gtcatcacgc gggtcaggtt cggttaagcc ttgctgccac gcctgatcca ccagttcggt
     4741 aaacggcacg ctaccgtcga attgcaggaa cagccaggag agcgtgccgg agaagatccc
     4801 actgatcgac aaaatggtat cgccgctgtc gatcagatcg cgcacggtat ggttgatcgg
     4861 caaacccgca ccgacggtgg cgttgtacag ccagtgacgc ccggtttttt cgaaggcgtc
     4921 gtggatctgg cgatatttat tgctgtcgct tgctcctgcc agtttgttgg cactgatgac
     4981 gtggaaaccg tggctggcga aatcaagata ctggtcagcg agctgctggc tggcggtaac
     5041 gtccagcacc actaaatcat catacggatg ggcgcgcatc cacaggaaca gcgactcttc
     5101 atcctgctcg accgcttcat cgttgaagaa ggctaacgcg cggctggcgt ccagaccgtc
     5161 atagctcaac aggctacggc ggctgtccac cacgcctgcc agcacaaatt caaagccggt
     5221 acgtgccgaa agcgtactct gctcacgggc gaacagttcc agccagcggg aaccgatatt
     5281 gcccttgccg aacaatacca ggccgatgcg tttttccgcg cggaagacgg actgatgcag
     5341 cccctgaatc aggctttcgg tcgggccggt gcgcagtact gccaccaggc tgatgccgtc
     5401 atcggactgc caggtaaatt cgaccggctg gcctttcagt tgctgccaga agcggtggca
     5461 atgcagcggg ttacgggtga cgcccgcccc aaccatcgcc accagcgcca gcccctgacg
     5521 caggcgcagt tcgccaggta atcccgcttc gtcgaggatt ttcagcgcac tgtcggccac
     5581 ttctgaggtg tagcaaaatt gcagcaactg gcgatcgtta tgcacgccaa ccgccagtgg
     5641 acgtacctga gcacgtttca ggatctggtc gatctcttta tgcgccagtt tgaaatcctg
     5701 gctggcgggc acctgaaact caatcaagca gacatcatca tggctggtga caatacgcgc
     5761 accagtaccg gaggccagca cgcgttcaat gcgcgtggaa ccttgatccg gcgtgtagct
     5821 acagcgcagt tgcaggtcga tttcgctgcc agaaacgggc tgtaaagtac gggcatgaag
     5881 aacgggagcc gccagacgcg ccagttcgct ggcttcatcc agacgcagca acggcagcag
     5941 gcaggcatct ttcactttac gcgggtcggc gctatatacc ccggcgacgt cgctccagat
     6001 ggttacgcga gaaacacccg ccagcgcacc gatttgtgtc gcggaatagt cggaaccgtt
     6061 acgccccagc agcaccgttt caccggcgtt gttacggctg ataaacccgg tcaccaccag
     6121 acgtttgccc ggatgttgta ccagtagctg ttgcagcaac gggtaagaaa gcccttcatc
     6181 aacctgcggt tgcgcagcgc gttcagcgcg taaaaactcg cgggcatcaa gccaggccga
     6241 tggcagccct tgttgattaa gcaccgcaga catcaggcgc gccgaccaca cttcaccgtg
     6301 gcccaccact tccgcataca ccgcatcgtt aataccgctg tcgagcaacg ccgccaggcg
     6361 ctcaagatcg ctgacaaaag cgctaatgag gttatcggct tcttcggcgg gtagcaggcc
     6421 gctaatcaga tcgcactgat aacgacgtaa tgtttgttga acctgatgcg cagagagacg
     6481 atcggtctgg cttagtttca accagttaat caactggtta gtggtgctac cggcggcgga
     6541 aaccaccatc atatcgtcag gctgagagta ctccgccata atgcccgcga cacgcaaata
     6601 acacttcaca tcagccagac tactgccacc aaatttatgc agttgacgac ctttcgcccc
     6661 tgcctgcgca atcacactca tttttacccc ttgtttgcag cccggaagcc attttccagg
     6721 tcggcaatta aatcttcgcc atcttcaata ccggtggaga tacgcagcag cgtctcggag
     6781 atcccggcgg cagcacgcgc ttctggtgcc atgcctgcat gtgtcatggt tgcggcgtga
     6841 gagattaaac tttccactcc ccctaatgat tccgccagcg taaacaacga cagcccgccc
     6901 aggaaacgac gcagcgtctg ctcatcgcca tccagttcaa aactcaacat tgcgccaaag
     6961 cctttttgct ggcgcgcggc aatttcatgc ccctgatttt ccggcagcga cgggtgatac
     7021 agtttcttca ccaacggctg ggtttgcaga tatttcacaa tcgcctgcgc gttgcgctgc
     7081 gccagctcca tacgcggcac cagcgttcgc aacccgcgca gcagcagata gctgtcaaac
     7141 gcgccgcccg tcacgccaat attgtttgcc caccaggcaa gttcagtgac aacgtccggg
     7201 tctttagcaa tcactacgcc tgccaccacg tcagagtgac cgttcagata tttcgtgcat
     7261 gaatgcaaca ccagatcggc acctaatgcc agtggatttt gtaatgccgg gcttaagaag
     7321 gtgttatcca ccacgctcac cgccccgact tcccttgcca gatggcaaat tttcgcaata
     7381 tccacgacgc gtaacaatgg attacttggg ctttctacca gtaccagttt gggtttttct
     7441 gccagcgctg cccgtaatgc ctgttcatcg ctttgatcaa caaacaacac gcgatagcaa
     7501 ccgcgtttcg ccaggctgtc gaacaggcga tagctaccgc cgtagcagtc gtgcggcgca
     7561 accagcagat cgccaggttt caaaaagacg gtcgttacca ggtgaatcgc ggacatgccg
     7621 gtattagtaa gtacggcacc ggcaccgcct tccagttctg ccagcgcacg ctgaaccaca
     7681 tcgcgcgttg ggttgccgcg acgcgagtaa tcatgcgcac gtggttcatt aaatccggta
     7741 aagttatagg tactggaaag atggatggga gggacaacgc aaccatactg ttcgtcgtca
     7801 tttaacccgc tacgcactgc gatggtggcc tgtttacgcg tcatgtgatg aagttccctg
     7861 ggctttgtcg gtgaaatgtc aggcaccaga gtaaacattg tgttaatgga cgtcaataca
     7921 tctggacatc taaacttctt tgcgtataga ttgagcaaat cccaaatagc cgttaaaatt
     7981 atatgcatta tcacgccgac aggtgcatta cacgatgtca cggtaacgcc tgtacggtaa
     8041 actatgcggg tttacggtca gtacccacat taactgtgtg gtctggtctc aatttattga
     8101 cgaagaggat taagtatctc atggctgaat ggagcggcga atatatcagc ccatacgctg
     8161 agcacggcaa gaagagtgaa caagtcaaaa agattacggt ttccattcct cttaaggtgt
     8221 taaaaatcct caccgatgaa cgcacgcgtc gtcaggtgaa caacctgcgt cacgctacca
     8281 acagcgagct gctgtgcgaa gcgtttctgc atgcctttac cgggcaacct ttgccggatg
     8341 atgccgatct gcgtaaagag cgcagcgacg aaattccgga ggcggcaaaa gagatcatgc
     8401 gtgagatggg gattaacccg gagacgtggg aatactaaag cggaaaagag cggcgcggag
     8461 ttaaccagcc gcttttttca tacatggacg tttaacgatg aaaatcaggc tactgatccc
     8521 cggcctgctg gtttctgtgc ctgcatttgc ctggcaacca caaaccggcg acatcatctt
     8581 tcagatctct cgctcatcgc aaagtaaagc gatccaactg gcgacccatt ccgattacag
     8641 ccacaccggt atgctggtga tacgcaacaa aaagccctac gtctttgaag ccgtcggccc
     8701 ggtaaaatat accccgctga aacagtggat cgcccacggt gaagatagca agtacatcgt
     8761 gcgtcgcgtt aaaggcgagc taaatgccga acaacagcaa aaactggcgc aaacggcaaa
     8821 acgttatctc ggtaaaccgt atgactccag cttctcgtgg agcgacgatc gccagtactg
     8881 ttcggaagtg gtgtggaagg tctaccagaa tgcgctggga atgcgtgtgg gcgagcagca
     8941 gaagctgaaa gagttcgatc ttagtaaccc gctagttcag gcaaagctca aagaacgcta
     9001 cggcaaaaat attccgcttg aggaaacggt agtttcacca caagccgttt tcgacgcgcc
     9061 acaactcacc acggtcgcca aagaatggcc gctgttttcc tggtaatacg accaaaattc
     9121 aggcacaaaa aaagcgccgt gcggcgcttt tttcggaaat ccggtcttat ttgctgcccg
     9181 ggatgttgaa acgcttgttg aagcggtcaa cacggccacc ggtagcaaca tcacgctgtt
     9241 tgccagtgaa gaacgggtgg cacttgctgc acacgtcgag gttcaggtca tgaccaacgg
     9301 tggagcggat tttcattacg ttaccgcaag agcagctagc agtaatttct tcgtatttcg
     9361 ggtgaatatc ttttttcatg ggaaaacctc ggtttaaggc cgcgtcgctc ttccagccct
     9421 aacgccagac accacgcgat gttaaaagta tagcttcaat acgatcattt cgtacgaagg
     9481 cgcgaaatca tacagaaatt aaccagcgta tgcaaactga tccgcactct tctacggcaa
     9541 tgtgtatact aacccaccga atttcaagtc aggatgatgc tatgcccgtt gcccacgttg
     9601 ccttgcccgt tccgcttcct cgtacctttg actatctgct gccagaaggc atgacggtta
     9661 aagccgggag tcgcgtgcgc gtgccgtttg gcaaacaaca ggagcgcatc ggggttgtgg
     9721 tgtcagtgag cgatgtcagt gagctgccgc ttaacgagct gaaagcggta gttgaagtgc
     9781 tggacgttga gccggtattt actcactccg tctggcgatt actgctctgg gcggcagatt
     9841 actatcatca tccgattggc gatgtgctgt ttcatgcctt gccgatttta ctgcgccagg
     9901 ggcggcctgc ggcaaacgcg cctatgtggt actggtttgc cactgaacaa ggtcaggcgg
     9961 tggatctgaa cagcctgaaa cgttcgccaa agcaacaaca ggcgctggca gcgttacggc
    10021 aaggcaaaat ctggcgcgat caggttgcca cgctcgaatt taatgatgcc gcgttgcagg
    10081 cactgcgcaa aaaaggtctg tgtgatttag caagtgaaac accagagttt agcgactggc
    10141 gaacgaacta tgccgtttct ggtgagcggt tgcggttgaa taccgaacag gccaccgccg
    10201 ttggcgcaat tcatagcgcg gcagatactt tttctgcctg gctgctggcg ggcgttaccg
    10261 gttccggtaa aacggaggtt tatctcagtg tactggaaaa cgtgctcgct cagggcaaac
    10321 aggcgctggt gatggtgccg gaaatcggcc tgacaccgca aactatcgcc cgttttcgtg
    10381 aacgttttaa tgccccggtg gaagttctgc attccggcct gaacgacagc gagcgtcttt
    10441 cggcgtggtt gaaagcgaaa aatggcgagg cggcgattgt catcggcacc cgctccgcgc
    10501 tgtttacgcc gtttaaaaat ctcggcgtga ttgttattga tgaagagcac gacagttcct
    10561 acaaacagca ggaaggctgg cgctatcatg cccgcgacct ggcggtgtat cgcgcgcata
    10621 gcgagcaaat cccaattatt cttggctccg caacgcccgc actggaaacg ttatgcaacg
    10681 tgcagcagaa aaaataccgc ctgctgcgcc tgacccgtcg ggcgggcaat gcgcgcccgg
    10741 caattcaaca tgtgctggat ttaaaaggtc agaaggtgca ggcaggtctg gctccggcgt
    10801 tgatcacccg tatgcgccag catttacagg ccgacaacca ggttatcctc tttcttaacc
    10861 gccggggttt tgcgcctgcg ctactgtgcc acgactgtgg ctggattgcc gaatgcccgc
    10921 gttgcgatca ctactacacg ctgcatcagg cacaacagca tttgcgctgt caccactgtg
    10981 acagccaacg cccggtgccg cgccagtgcc cttcctgcgg ttccacacac ctggtacccg
    11041 tggggctggg caccgaacag cttgaacaga cgctcgcgcc gctgttcccc ggcgtgccca
    11101 tttctcgtat cgaccgcgat accaccagcc gcaaaggggc gctggaacag caactggcgg
    11161 aagtgcatcg cggcggcgcg aggatcttga ttggcacgca aatgctggcg aaaggtcacc
    11221 actttccgga tgtgacgctg gtagcgttac tggatgtgga cggcgcgctg ttttctgccg
    11281 attttcgctc ggcagagcgt ttcgctcagc tttacaccca ggtcgctggt cgtgccgggc
    11341 gtgcgggtaa acagggcgaa gtggtgctgc aaacgcacca tccggaacat cctctgttgc
    11401 aaacgttgct ctataaaggc tacgacgact ttgccgaaca ggcgctggct gagcgacgga
    11461 tgatgcagct gccgccatgg accagccatg tgattgtgcg tgcggaagat cataacaatc
    11521 agcacgcgcc gctgttcctg caacagttgc gtaatctgat cctcgccagc ccgcttgcag
    11581 acgacaaact gtgggttctc ggtccggttc cggcactggc ccctaagcgt ggtggccgct
    11641 ggcgctggca aattttgttg cagcaccctt cccgcgtgcg attacagcac atcatcaatg
    11701 gtacgctggc gcttattaat acaataccgg attcccgtaa agtgaaatgg gtgctggatg
    11761 tcgatcccat cgagggctaa accgctcacg atgcgaggcg gatcgaaaaa ttcaatattc
    11821 atcacacttt tcatgaaaat tctgtaaccg ttttcacgcg ctatctgcta aaaatgttgc
    11881 cgatgtgaag taaacatgga tgtagtacgc ctgacgtgcc aggcgaggag tgagtgtgaa
    11941 agcgaagaag caggaaactg ccgcgaccat gaaagacgtt gccctcaagg caaaagtctc
    12001 tacagcgact gtctcccgag cattaatgaa tcccgataaa gtctcccagg ccacccgtaa
    12061 tcgggttgaa aaagcggccc gggaagtggg ttatttaccg cagcctatgg ggcgcaacgt
    12121 caagcgtaat gaatcccgca ccattctggt gattgtcccg gatatctgcg accctttctt
    12181 tagcgaaatt attcgcggta tcgaagttac ggcggcaaat cacggatatc tggtgctgat
    12241 tggcgactgt gcgcatcaaa atcagcagga aaaaaccttt atcgatttga tcatcaccaa
    12301 gcaaattgat ggcatgttgt tgctgggttc aaggctgccg tttgatgcca gcattgagga
    12361 acagcgtaat ctgccgccga tggtgatggc gaacgaattt gcaccggaac tggagctgcc
    12421 taccgttcat atcgacaatc tgaccgccgc atttgatgca gtaaattatt tatatgagca
    12481 agggcataaa cggattggct gtatagccgg tcccgaagag atgccgctgt gtcactaccg
    12541 cctgcaaggc tatgttcagg cgctgcgtcg ctgcggcatt atggttgatc cgcaatacat
    12601 cgcccgtggc gacttcacct tcgaagccgg aagcagagca atgcaacagc tgcttgatct
    12661 tccacaaccg cctactgctg tcttctgcca tagcgatgtg atggcgctcg gcgcactttc
    12721 tcaggcaaaa cgtcaggggc tgaaagtccc ggaagacctt tccataatcg gttttgataa
    12781 catcgacctg acgcaatttt gtgatccgcc gctgacaacc atcgcgcagc cgcgttacga
    12841 aatcggtcgg gaagctatgc tgttattgct tgatcaaatg caggggcaac acgttggcag
    12901 tggctctcgt ttaatggact gcgaacttat catccgggga tcaacacgcg cgttacctta
    12961 aagtaaacct ttaagactgc cgtgactggt caaaggcccg ccgcttaagt aacatggcgg
    13021 gctgacgaac gaataaatac agcgaaacga tagtggcaca acgagattat gtacgccgca
    13081 gccaaccggc accttcgcgg cgaaaaaaga gcacctcacg gaaaaagcaa cgaaatctgc
    13141 ctgcggtttc tcccgctatg gtcgctattg ctgccgccgt tcttgtgacc tttatcggtg
    13201 gtctgtactt cattacgcat cacaagaaag aagagtccga gacgctgcaa agccagaaag
    13261 tgaccggaaa cggactacca ccaaaaccag aagaacgctg gcgctacatt aaagagctgg
    13321 aaagtcgcca gccgggtgtg cgtgcgccca cagaaccttc tgccggtggt gaagtgaaaa
    13381 cgccggagca actgacacca gaacaacgcc agcttcttga acaaatgcag gccgatatgc
    13441 gccagcagcc aacgcagctg gttgaagttc cgtggaatga gcagacgccg gaacagcgtc
    13501 agcaaacgct acagcgccag cgtcaggcgc agcaattagc ggagcagcaa cgcctggctc
    13561 agcagtcgcg tacgactgaa caaagctggc agcagcagac gcgtacgtcg caagccgcgc
    13621 cggtacaggc gcaaccgcgc cagtctaaac cggcttctac tcagcagccg taccaggatc
    13681 tgctgcaaac ccctgcgcac acgaccgcgc agtcgaagcc gcagcaagct gcgccggttg
    13741 ctcgtgccgc tgacgcgcca aaaccgacgg cggagaaaaa agacgaacgc cgctggatgg
    13801 tgcagtgcgg ttcgttcaga ggcgcggaac aggcagagac ggtacgtgct cagctggcgt
    13861 tcgaaggctt tgactcaaaa attaccacca acaatggctg gaatcgtgtg gtgattggcc
    13921 cggtgaaagg caaagagaac gcagacagca ccctcaatcg cttgaagatg gcgggtcata
    13981 caaactgcat tcggctcgcc gccgggggtt gaaaccctca aaatcccccc catctataat
    14041 tgcattatgc cccgtacttt tgtacggggt ttgtactctg tattcgtaac caaggggtca
    14101 gctcgtgaca actatagtaa gcgtacgccg taacggccat gtggtcatcg ctggtgatgg
    14161 tcaggccacg ttgggcaata ccgtaatgaa aggcaacgtg aaaaaggtcc gccgtctgta
    14221 caacgacaaa gtcatcgcgg gctttgcggg cggtactgcg gatgctttta cgctgttcga
    14281 actgtttgaa cgtaaactgg aaatgcatca gggccatctg gtcaaagccg ccgttgagct
    14341 ggcgaaagac tggcgtaccg atcgcatgct gcgcaaactt gaagcactgc tggcagtcgc
    14401 ggatgaaact gcatcgctta tcatcaccgg taacggtgac gtggtgcagc cagaaaacga
    14461 tcttattgct atcggctccg gcggccctta cgcccaggct gcggcgcgcg cgctgttaga
    14521 aaacactgaa cttagcgccc gtgaaattgc tgaaaaggcg ttggatattg caggcgacat
    14581 ttgcatctat accaaccatt tccacaccat cgaagaatta agctacaaag cgtaaggatc
    14641 tcccatgtct gaaatgaccc cacgcgaaat cgtcagcgaa ctggataagc acatcatcgg
    14701 ccaggacaac gccaagcgtt ccgtggcgat tgctctgcgt aaccgctggc gtcgcatgca
    14761 gctcaacgaa gagctgcgcc atgaagtgac cccgaaaaat atcctgatga tcggcccgac
    14821 cggtgtcggt aaaactgaaa tcgcccgtcg tctggctaag ctggcgaatg cgccgttcat
    14881 caaagttgaa gcgaccaaat tcaccgaagt gggctatgtc ggtaaggaag tggattctat
    14941 tattcgcgat ctgaccgatg ccgccgtgaa aatggtacgc gtccaggcta tcgagaaaaa
    15001 ccgttatcgc gctgaagaac tggcagaaga acgtattctc gacgtactga tcccaccggc
    15061 taaaaataac tggggacaga ccgaacagca gcaggagccg tccgctgctc gtcaggcatt
    15121 ccgcaaaaaa ctgcgtgaag gccagcttga tgacaaagaa attgagatcg atcttgccgc
    15181 agcaccgatg ggcgttgaaa ttatggctcc tccgggcatg gaagagatga ccagccagct
    15241 gcagtccatg ttccagaacc tgggcggtca gaagcaaaag gcgcgtaagc tgaaaatcaa
    15301 agacgccatg aagctgctga ttgaagaaga agcggcgaaa ctggtgaacc cggaagagct
    15361 gaagcaagat gctatcgacg ctgttgagca gcacgggatc gtgtttatcg acgaaatcga
    15421 caaaatctgt aagcgcggcg agtcttccgg tccggatgtt tctcgtgaag gcgttcagcg
    15481 tgacctgctg ccgctggtag aaggttgcac tgtttccacc aaacacggga tggtcaaaac
    15541 tgaccacatt ctgtttatcg cttctggcgc gttccagatt gcgaaaccgt ctgacctgat
    15601 cccggaactg caaggtcgtc tgccaatccg cgttgagctg caggcactga ccaccagcga
    15661 ctttgagcgt attttgaccg agccgaatgc ctctatcacc gtgcagtaca aagcactgat
    15721 ggcaactgag ggcgtaaata tcgagtttac cgactccggt attaagcgca tcgcggaagc
    15781 ggcatggcag gtgaacgaat ctaccgaaaa catcggtgct cgtcgtttac ataccgttct
    15841 ggagcgttta atggaagaga tttcctacga cgccagcgat ttaagcggtc aaactatcac
    15901 tattgacgca gattatgtga gcaaacatct ggatgcgttg gtggcagatg aagatctgag
    15961 ccgttttatc ctataatcgc gttcaatcat tttcatcatt gtttgatggg gctgaaaggc
    16021 cccattttta ttggcgcgta ttatgactga acaacaaatt agccgaactc aggcgtggct
    16081 ggaaagttta cgacctaaaa ccctccccct cgcctttgct gcaattatcg tcgggacggc
    16141 gctggcatgg tggcaaggtc acttcgatcc actggtcgcc ctgctggcat tgattaccgc
    16201 cgggctatta cagatccttt ctaacctcgc caatgattac ggcgatgcgg taaaaggcag
    16261 cgataaacct gaccgcattg ggccgctacg cggcatgcaa aaaggggtca ttacccagca
    16321 agagatgaaa cgggcgctca ttattaccgt cgtgctcatc tgtctctccg ggctggcact
    16381 ggttgcagtg gcgtgccata cgctggccga ttttgtcggt ttcctgattc ttggcgggtt
    16441 gtcgatcatt gccgctatca cctacaccgt gggcaatcgt ccttatggtt atatcggttt
    16501 gggtgatatt tccgtactgg ttttctttgg ctggttgagc gtcatgggga gctggtattt
    16561 acaggctcat acattgattc cggcactgat ccttccggca accgcatgcg gcctgctggc
    16621 aacggcagta ctgaacatta ataacctgcg tgatatcaat agcgaccgcg aaaacggcaa
    16681 aaacacgctg gtggtgcgct taggtgcagt aaacgcgcgt cgttatcatg cctgcctgct
    16741 gatgggctca ctggtgtgtc tagcgctgtt taatctcttt tcgctgcata gcctgtgggg
    16801 ctggctgttc ctgctggcgg caccgttgct ggtgaagcaa gctcgttatg tgatgcgcga
    16861 aatggacccg gtggcgatgc gaccaatgct ggaacgcact gtcaaaggag cgttactgac
    16921 taacctgctg tttgttttag ggatattcct aagccagtgg gcagcataac tgacaaatat
    16981 caattaacaa ttgatgattt tgccaacagc ccacatagcg cgatatactg aaaattctcg
    17041 cagcaactga atgttaagcc tatgaaatac gatacttccg agctttgtga catctatcaa
    17101 gaagatgtta acgtcgtgga accgctgttc tccaactttg gcggacgggc gtcgtttggc
    17161 ggacaaataa tcacggtaaa atgtttcgag gacaacgggt tgctgtacga tctgctcgaa
    17221 cagaatggcc gtggtcgtgt tcttgtcgtc gatggcggag gttctgttcg ccgcgcactg
    17281 gtcgatgctg aactggcgcg tctggcagta caaaatgaat gggaaggcct ggtcatttac
    17341 ggcgcggtgc gtcaggtaga tgacctggaa gagttggata tcggcatcca ggcgatggcg
    17401 gcaattccgg ttggtgccgc tggcgaaggc attggcgaaa gcgatgtccg cgtcaatttt
    17461 ggcggtgtca ccttcttctc cggcgaccat ctttatgccg acaataccgg gattattctt
    17521 tcagaagatc cgctggatat tgaatgataa aaaaggcacc gcaaggtgcc ttttttctgc
    17581 gttacctgtt ggcctacaca gtaaagaaat tacgcgaaag atgaagtgta atcagacctc
    17641 ttccatgcga cccagcaggg cctgcagacg ttcctgccag ccgttctgct gttctttcag
    17701 atggttgttc tcacgctcca gctcttcgcg ctgatgctgg gcattttgaa cttcctgcga
    17761 cagtgagttg tttttttctt tcagctcttc gatttccatc tgcaacagag tgatggtatc
    17821 aatcgcctgc tgtacttttg cttccagttt ctcaaacact tctaatgaca ttgtcatacc
    17881 tctcctgaat tgcaaggcgt tgatggataa aaatcctcgt cccgattacc ggtgacgcct
    17941 taataaatac gagcgcactt tagttagctc cgattgtatg aagccgcgcc atcgctgtcc
    18001 agcggcacgc cttgcagatt acggtttgcc acacttttca tccttctcct ggtgacataa
    18061 tccacatcaa tcgaaaatgt taataaattt gttgcgcgaa tgatctaaca aacatgcatc
    18121 atgtacaatc agatggaata aatggcgcga taacgctcat tttatgacgg agcacacaca
    18181 ttttaagttc gatatttctc gtttttgctc gttaacgata agtttacagc atgcctacaa
    18241 gcatcgtgga ggtccgtgac tttcacgcat acaacaaaca ttaactcttc aggatccgat
    18301 tatgagtcaa acatcaacct tgaaaggcca gtgcattgct gaattcctcg gtaccgggtt
    18361 gttgattttt ttcggtgtgg gttgcgttgc agcactaaaa gtcgctggtg cgtcttttgg
    18421 tcagtgggaa atcagtgtca tttggggact gggggtggca atggccatct acctgaccgc
    18481 aggggtttcc ggtgcgcatc ttaatcccgc tgttaccatt gcattgtggc tgtttgcctg
    18541 tttcgacaag cgcaaagtta ttccttttat cgtttcacaa gttgccggcg ctttctgtgc
    18601 tgcggcttta gtttacgggc tttactacaa tttatttttc gacttcgagc agactcatca
    18661 cattgttcgc ggcagcgttg aaagtgttga tctggctggc actttctcta cttaccctaa
    18721 tcctcatatc aattttgtgc aggctttcgc agttgagatg gtgattaccg ctattctgat
    18781 ggggctgatc ctggcgttaa cggacgatgg caacggtgta ccacgcggcc ctttggctcc
    18841 cttgctgatt ggtctactga ttgcggtcat tggcgcatct atgggcccat tgacgggctt
    18901 tgccatgaac ccagcgcgtg acttcggtcc gaaagtcttt gcctggctgg cgggctgggg
    18961 caatgtcgcc tttaccggcg gcagagacat tccttacttc ctggtgccgc tttttggccc
    19021 tatcgttggc gcgattgtag gtgcatttgc ctaccgcaaa ctgattggtc gccatttgcc
    19081 ttgcgatatc tgtgttgtgg aagaaaagga gaccacaact ccttcagaac aaaaagcttc
    19141 gctgtaatat gactacggga caattaaaca tgactgaaaa aaaatatatc gttgcgctcg
    19201 accagggcac caccagctcc cgcgcggtcg taatggatca cgatgccaat atcattagcg
    19261 tgtcgcagcg cgaatttgag caaatctacc caaaaccagg ctgggtagaa cacgacccaa
    19321 tggaaatctg ggccacccaa agctccacgc tggtagaagt gctggcgaaa gctgatatca
    19381 gttccgatca aattgcagct atcggtatta cgaaccagcg tgaaaccact attgtctggg
    19441 aaaaagaaac cggcaaacct atctataacg ccattgtctg gcagtgccgt cgtaccgccg
    19501 agatctgcga acatctcaaa cgcgatggca tggaagagta cattcgtaac aacaccggtc
    19561 tggtgattga cccgtacttc tctggcacca aagtgaagtg gatcctcgac catgtggaag
    19621 gctctcgcga gcgtgcgcgt cgtggtgaat tgctgtttgg tacggttgat acgtggctta
    19681 tctggaaaat gactcagggc cgtgtccatg tgaccgatta caccaacgcc tctcgtacca
    19741 tgttgtttaa catccatact ctggactggg acgacaaaat gctggaggtg ctggatattc
    19801 cacgcgagat gctgccagaa gtgcgtcgtt cttccgaagt atacggtcag actaacattg
    19861 gcggcaaagg cggcacgcgt attccaatct ccgggatcgc cggtgaccag caggcggcgc
    19921 tgtttggtca gttgtgcgtg aaagaaggaa tggcgaagaa cacctacggt accggctgct
    19981 ttatgctgat gaacactggc gagaaagcgg tgaaatcaga aaacggcctg ctgaccacca
    20041 tcgcctgcgg cccgactggc gaagtaaact atgcgctgga aggtgcggtg tttatggcgg
    20101 gcgcgtccat tcagtggctg cgcgatgaga tgaagttgat taacgacgcc tacgattccg
    20161 aatattttgc caccaaagtg caaaacacca atggtgtgta tgtggttccg gcctttaccg
    20221 ggctgggtgc gccatactgg gatccgtatg cacgcggggc gattttcggt ctgactcgtg
    20281 gggtgaacgc taaccacatt atacgcgcga cgctggagtc tattgcttat cagacgcgtg
    20341 acgtgctgga agcaatgcag gccgactccg gtattcgtct gcacgcgctg cgcgtggacg
    20401 gcggtgcggt ggcgaacaat ttcctgatgc agttccagtc cgatattctc ggtacgcgcg
    20461 ttgagcgccc ggaagtgcgc gaagtcaccg ctttgggtgc ggcctatctt gctggtctgg
    20521 cggttggctt ctggcagaat cttgacgagc tgcaagagaa agcggtgatt gaacgcgagt
    20581 tccgtccagg catcgaaacc actgagcgta attaccgtta cgcaggctgg aaaaaagcgg
    20641 tcaaacgcgc gatggcatgg gaagaacacg acgagtaata atgattgcct gatgcgacgc
    20701 ttgcgcgtct tatcaggcct acgagtcgga tacacatccg gcatcacgca gactctgaaa
    20761 ccgtgcgggg gcaaccccgc acacatcaat aatcccgccc ttcccctgtg ctacacttcg
    20821 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    20881 cgtcaccgaa tcagcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    20941 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca acattgacgg
    21001 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    21061 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    21121 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    21181 tttcctcaac gcgccagata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    21241 caccattgat ctgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    21301 caaaccgttg agcgaactga cggtaacgat tctggcaaaa ccgcgccacg atgccgttat
    21361 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggacggcg acgttgcagc
    21421 ctcaattctc acctgtatgc cggacagtga agtcgatgtg ctgtacggca ttggcggcgc
    21481 gccggaaggc gtggtttctg cggcggtgat ccgcgcatta gatggcgaca tgaatggtcg
    21541 tctgctggca cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    21601 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gtttgggcga
    21661 tatggcgcgc agcgacaacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    21721 ggaaggcatt agccgcaaag gcaatatcgc caccaccgaa acgctgctga tccgtggtaa
    21781 atcacgcacc attcgtcgca ttcagtccat ccactatctg gatcgtaaag acccggaaat
    21841 gcaggtgcac attctctgag tgatttgatc gattgagcct tccagtcctt cgggactgga
    21901 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    21961 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    22021 caccgttcac gctcccgtgc ttccgtttac cgccgggcaa ttcaccaaac ttggccttga
    22081 aattgaaggc gaacgcgttc agcgtgccta ctcttacgtt aactcgcccg acaatcccga
    22141 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccgcgac tggcggcgct
    22201 aaaaccgggt gatgaagtgc aagtggttag tgaggcggca ggcttctttg tgctggatga
    22261 agtccctgac tgcgaaacac tgtggatgct ggcgactggt acggcgattg gcccttattt
    22321 atcgattctg caattgggca aagatttaga acgcttcaaa aacatggtgc tggttcacgc
    22381 cgcacgatat gccgccgact taagctatct acctctgatg caggaactgg aaaaacgcta
    22441 cgaggggaaa ctgcgcattc agacggtggt cagtcgggaa acggctgcgg gttcgctcac
    22501 tgggcggata ccggcgttaa ttgaaagtgg tgaactggaa agcgcggttg gcctgccgat
    22561 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    22621 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    22681 gacagcggag cattactggt aagcggttac ttgtcgataa acggcacgat caagagatcc
    22741 gcactcaact tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    22801 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    22861 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    22921 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    22981 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    23041 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    23101 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    23161 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaagg
    23221 tgaaggccgc atcaagctca acttgccctt ccagatcttt aatcttatag gccacgctgg
    23281 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaggact
    23341 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    23401 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    23461 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    23521 aaggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    23581 tgaagagttc agaaggattt tccgggcatt gtcacttgca tcgtggaaat aacaatcccg
    23641 gaaaaaaata gcgataagag atatattaaa ggttaattac ttg
//
LOCUS       DACADF010000072        23490 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.072, whole genome shotgun sequence.
ACCESSION   DACADF010000072 DACADF010000000
VERSION     DACADF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.072"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..562
                     /locus_tag="ISB30_002670"
     CDS             <1..562
                     /locus_tag="ISB30_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAP1990286.1"
                     /translation="KSKIEELEKQQEQYKNKAELFEKQQNNITNSTRILASIVKTVKE
                     KQDEFVFTEFNPLQTQYFILNNGSVGLAGRVLAIDAVENGSVIRISLVNLLSVPVSNI
                     GFHATWGNEKPTDAKALAKWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYL
                     KLSINMANLQFDTVQPAETRQRKNKK"
     gene            606..4949
                     /locus_tag="ISB30_002671"
     CDS             606..4949
                     /locus_tag="ISB30_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAP1990287.1"
                     /translation="MNKIFKVIWNPATGSYNVASETAKSRGKKSGRSKLLISALVAGG
                     LLSSFGALADNYEGQPVDYGNGSAGDGWVAIGKNSKANTFVNGSGASTALGYDAIAEG
                     EYSSAIGSRTHAIGGASMAFGVSAQAIGDRSVALGASSVATGGRSMALGRYAKTNGFT
                     SLAIGDTALADGEKTIALGNTAQAYEIMSIALGDHANASKEYSMALGASSVASAANAI
                     AVGRDSAANGTDSLALGRLSLASAANAIAMGAESEAAENATAIGFNADAIGKSSLALG
                     DNASAGETNSIAIGQGSEASKLDSIALGSNSRSAGENAIALGNNSNAGGKNSLAFGFN
                     TTANGDNAVAIGANSSAGADNTVSVGSSSLKRKIVNMGNGDINNVSSDAINGSQLYAI
                     SKSVSDRLGGYHDDPDNVINSDGTLKAPTYYLQSGQYNNVGEALQSIDNNTLHWDSKS
                     NKYSASHTVFNANGSVKSTSAKNIITDVADGTISATSSDAVNGSQLYNLKQDALLWDG
                     TAFSAKHGTSNTNSKITNVADGAVSASSKDAVNGSQLYDLKQDALLWDGTAFSAKHGT
                     SNTDSKITNVLAGTVSSTSTDAINGTQLQSTASTITSYLGGGAAMSNDGVFTGPQYNI
                     GGKNYTNVGAALTALDNDALLWDATANSNTGAFSAAHGTLKTASKITNVANGDILANS
                     YDAINGSQLYSLADSFTSYLGGGADINNAGVLIAPTYTIGSKTYNNVGDALNAINSSF
                     STSISDALLWDSTANKFSAKHGGTNSIITNVADGTISSTSSDAVNGSQLYDTSKYIAD
                     ALGGDAEVNADGTMTAPTYSIANEDYHSVGEALDALDDNALLWDEDAGAYNASHDGNA
                     SKITNVAAGDLSTTSTDAVNGSQLNATNILVTQNSQMINQLAGNTSETYIEENGAGIN
                     YVRTNDNGLAFNDASASGIGATAVGYNSVAEGDSSVAIGQGSSSSVDTGIALGSSSVS
                     SRVIVKGSRNTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVT
                     VRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLV
                     LADAINGIAIGSNARANHADSIAMGNGSQTTRGAQTNYTAYNMDAPQNSVGEFSVGSE
                     DGQRQITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNTQVTNLDTRVTNIENG
                     IGDIVTTGSTKYFKTNTDGADANAQGKDSVAIGSGSIAAADNSVALGTGSVADEENTI
                     SVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGN
                     GGTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIA
                     SAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGE
                     YSAALGAGIQW"
     gene            5367..7022
                     /gene="lldP"
                     /locus_tag="ISB30_002672"
     CDS             5367..7022
                     /gene="lldP"
                     /locus_tag="ISB30_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAP1990288.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFTSGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7022..7798
                     /gene="lldR"
                     /locus_tag="ISB30_002673"
     CDS             7022..7798
                     /gene="lldR"
                     /locus_tag="ISB30_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAP1990289.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFVGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            7795..8985
                     /gene="lldD"
                     /locus_tag="ISB30_002674"
     CDS             7795..8985
                     /gene="lldD"
                     /locus_tag="ISB30_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAP1990290.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            9033..9506
                     /gene="trmL"
                     /locus_tag="ISB30_002675"
     CDS             9033..9506
                     /gene="trmL"
                     /locus_tag="ISB30_002675"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP1990291.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(9559..10380)
                     /gene="cysE"
                     /locus_tag="ISB30_002676"
     CDS             complement(9559..10380)
                     /gene="cysE"
                     /locus_tag="ISB30_002676"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP1990292.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10460..11479)
                     /gene="gpsA"
                     /locus_tag="ISB30_002677"
     CDS             complement(10460..11479)
                     /gene="gpsA"
                     /locus_tag="ISB30_002677"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP1990293.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11479..11946)
                     /gene="secB"
                     /locus_tag="ISB30_002678"
     CDS             complement(11479..11946)
                     /gene="secB"
                     /locus_tag="ISB30_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAP1990294.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12010..12261)
                     /gene="grxC"
                     /locus_tag="ISB30_002679"
     CDS             complement(12010..12261)
                     /gene="grxC"
                     /locus_tag="ISB30_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAP1990295.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12402..12833)
                     /locus_tag="ISB30_002680"
     CDS             complement(12402..12833)
                     /locus_tag="ISB30_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP1990296.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13077..14621
                     /gene="gpmM"
                     /locus_tag="ISB30_002681"
                     /gene_synonym="pgmI"
     CDS             13077..14621
                     /gene="gpmM"
                     /locus_tag="ISB30_002681"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAP1990297.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRTFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14631..15914
                     /gene="envC"
                     /locus_tag="ISB30_002682"
     CDS             14631..15914
                     /gene="envC"
                     /locus_tag="ISB30_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAP1990298.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15918..16877
                     /locus_tag="ISB30_002683"
     CDS             15918..16877
                     /locus_tag="ISB30_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAP1990299.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQLVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(16864..17898)
                     /gene="waaH"
                     /locus_tag="ISB30_002684"
     CDS             complement(16864..17898)
                     /gene="waaH"
                     /locus_tag="ISB30_002684"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAP1990300.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(18137..19162)
                     /gene="tdh"
                     /locus_tag="ISB30_002685"
     CDS             complement(18137..19162)
                     /gene="tdh"
                     /locus_tag="ISB30_002685"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP1990301.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(19172..20368)
                     /gene="kbl"
                     /locus_tag="ISB30_002686"
     CDS             complement(19172..20368)
                     /gene="kbl"
                     /locus_tag="ISB30_002686"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP1990302.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPELIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMHE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            20582..21514
                     /gene="rfaD"
                     /locus_tag="ISB30_002687"
     CDS             20582..21514
                     /gene="rfaD"
                     /locus_tag="ISB30_002687"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAP1990303.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            21524..22570
                     /gene="rfaF"
                     /locus_tag="ISB30_002688"
     CDS             21524..22570
                     /gene="rfaF"
                     /locus_tag="ISB30_002688"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAP1990304.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            22574..>23490
                     /gene="rfaC"
                     /locus_tag="ISB30_002689"
     CDS             22574..>23490
                     /gene="rfaC"
                     /locus_tag="ISB30_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAP1990305.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSATL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPRENLI"
BASE COUNT         5850 a         6111 c         6105 g         5424 t
ORIGIN      
        1 aaaaagtaaa atagaggagc ttgagaaaca acaagaacaa tataaaaata aagcagaact
       61 gtttgaaaaa caacaaaata atatcacaaa cagcaccaga atacttgcca gcattgtaaa
      121 gacagtcaaa gaaaaacagg acgaatttgt cttcaccgaa tttaacccgc tacaaaccca
      181 atacttcatt ttaaataacg gctcggtcgg tttggcaggc cgggtgctgg ctattgatgc
      241 ggttgaaaat ggcagtgtca ttcgtatttc gctggttaac ttattaagcg ttcccgtatc
      301 aaatattggc tttcatgcga catgggggaa tgagaaaccc accgatgcca aagctctggc
      361 gaaatggcaa caattactat tcaacaccac cctgaattcc actctgcaat taatgcctgg
      421 tcaatggcaa gacattaatc tgacattaaa aggcgtctcg cctaataacc tgaagtatct
      481 gaagttgtcc atcaatatgg ctaatcttca gttcgacact gttcaacctg ctgaaacccg
      541 gcagcggaaa aacaaaaaat aaatgcagct tctgcgctta tatcaaggaa tagagagcat
      601 caataatgaa caaaatattt aaagttatct ggaatccggc gacaggcagt tacaacgtcg
      661 ccagcgaaac ggcgaagagc cgtggcaaaa agagcgggcg cagtaagctg ttaatttctg
      721 ccttggttgc aggtgggctg ttatcgtcat ttggggcact ggctgataac tacgaggggc
      781 agccagtgga ttatggcaat ggctcagcag gtgacggctg ggttgctatc ggtaaaaatt
      841 caaaagcaaa tacctttgtc aatggctctg gtgcgagcac tgcgctaggt tatgacgcga
      901 tagctgaagg tgagtacagc tcagctatag gctcacgaac tcatgcgatt ggtggtgcat
      961 caatggcctt tggggttagt gcacaagcaa taggtgacag aagtgtcgcg ctgggtgcat
     1021 cttcagtagc aactggcggt cgttcgatgg ctcttggtcg ttacgcaaag acgaatggtt
     1081 ttacatctct tgctattggg gacaccgccc ttgccgatgg tgaaaaaact attgcgttag
     1141 gaaatacggc tcaagcttac gaaattatga gcatcgccct cggtgatcat gctaatgcct
     1201 caaaagaata ttcaatggcg ctgggagcaa gtagcgttgc atctgcggct aacgccattg
     1261 ccgtaggccg cgacagtgct gctaacggca cagacagcct tgccttaggc agactatctc
     1321 tcgccagcgc agcaaacgcg attgctatgg gtgcagagag cgaagctgct gaaaatgcga
     1381 cggcgattgg ctttaacgcg gatgcgatcg ggaagtccag cctggcgctg ggagacaacg
     1441 ccagcgctgg tgaaaccaac tccatcgcta taggtcaagg cagcgaggca agcaaacttg
     1501 attcaattgc gctgggaagt aacagcaggt cagcgggtga gaacgccatt gcactgggca
     1561 acaacagtaa tgcggggggc aaaaacagcc ttgctttcgg ttttaacacc accgcaaatg
     1621 gtgataacgc agtcgccatt ggtgcaaatt cttccgcagg tgctgacaat accgtgtctg
     1681 taggtagctc tagtttaaag cgcaaaattg tcaatatggg taatggtgac atcaataatg
     1741 tcagctccga tgccatcaat ggatcacaac tttatgccat cagtaagtct gtatccgaca
     1801 ggcttggagg atatcatgat gatcctgata atgttataaa ctctgacggg acgcttaaag
     1861 ctcccacata ttatctacaa agtggccaat acaataacgt gggagaggct ctccagagca
     1921 tcgacaataa cacgttgcac tgggacagca aaagtaataa atacagtgcc agccatactg
     1981 tctttaatgc aaatggctca gttaaaagca cctcggcgaa aaacatcatt accgacgtcg
     2041 ctgatggcac gatttcagcc accagttccg acgccgttaa cggttcgcag ttgtataatc
     2101 tgaaacaaga tgccttgcta tgggatggca ctgcgtttag cgccaaacat ggcaccagca
     2161 atactaacag caaaatcacc aacgttgcgg atggtgctgt ttcagcctcc agtaaagatg
     2221 ccgttaacgg ttcgcagttg tatgatctga aacaagatgc cctgctatgg gatggcactg
     2281 cgtttagcgc caaacatggc accagcaata ctgacagcaa aatcaccaac gtactggcag
     2341 gcacagtatc ttccaccagc accgatgcca ttaatggtac acaacttcaa agcactgcaa
     2401 gcaccattac gtcctatttg ggcggcggcg cggcaatgag taatgacggc gtatttaccg
     2461 gtcctcaata taatattggt ggtaagaatt acaccaatgt tggcgcagct cttaccgctc
     2521 tggacaatga cgccctgttg tgggatgcaa ccgctaattc aaatactggc gcattcagtg
     2581 ctgcacacgg aacactcaaa accgccagca agattaccaa cgttgcaaat ggtgacatat
     2641 tagccaacag ttatgatgct attaatggct cacaacttta tagtttagct gattcattta
     2701 cgtcatacct tggcggtggt gctgatatca acaatgcggg tgttctcata gccccgacct
     2761 atactatcgg ttcgaaaacg tacaacaacg taggtgatgc ccttaacgcg attaactcat
     2821 cattcagcac ctcaatcagt gatgccctgc tgtgggattc tacagcgaac aaattcagcg
     2881 ccaaacacgg tggtaccaat agcattatta ctaacgttgc agacggtaca atttcctcta
     2941 ccagcagcga tgctgtaaat ggctcgcaac tctatgacac gagcaagtac attgctgatg
     3001 ccctgggtgg tgacgcagaa gttaacgctg acggcaccat gactgcaccg acttactcta
     3061 tcgctaatga agattaccac agcgtaggtg aagccctgga tgcgctcgat gataacgcgc
     3121 tgctgtggga tgaagacgca ggtgcctaca acgccagcca tgatggcaat gccagcaaaa
     3181 ttaccaacgt tgcggcaggt gatctctcca caaccagtac cgatgctgtt aacggttccc
     3241 agttaaacgc aaccaatatt ctggttacgc aaaatagcca aatgattaac cagcttgccg
     3301 gtaacaccag cgaaacctac atcgaggaaa acggtgcggg tattaactat gtacgtacca
     3361 acgacaacgg cttagcgttc aacgatgcca gcgcctcagg tattggtgct acggctgtgg
     3421 gttataactc tgttgccgaa ggtgatagca gcgtggccat tggtcaaggt agcagcagct
     3481 ccgttgatac cggcattgcc ttgggtagca gctccgtttc cagtcgcgtg attgttaaag
     3541 gttctcgtaa cactagtgta acggaaaatg gcgtagttat cggttatgac accactgacg
     3601 gtgagttgct cggtgcattg tcaattggtg atgacggtaa ataccgtcaa atcatcaacg
     3661 tcgccgatgg ttccgaagcc catgacgccg ttacggttcg ccagctgcaa aatgccattg
     3721 gtgcagtcgc taccacgccg actaaatact tccacgctaa ctcaacggct gaagactcac
     3781 tggcagtcgg tgaagactcg ctggcaatgg gcgcgaaaac catcgttaat ggtaatgcgg
     3841 gtattggtat cggcctgaac acgctggttc tggctgatgc gatcaacggt attgctatcg
     3901 gttctaacgc acgcgcaaat catgccgaca gcattgcaat gggtaatggt tctcagacta
     3961 cccgtggtgc gcagaccaac tacactgcct acaacatgga cgcgccgcag aactccgtgg
     4021 gtgagttctc tgtcggcagt gaagacggtc aacgtcagat caccaacgtc gcagcggggt
     4081 cggcggatac cgatgcggtc aacgtgggtc aattgaaagt aacggacgcg caggtttccc
     4141 agaataccca gagcattact aacctgaaca ctcaggtcac taatctggat actcgcgtga
     4201 ccaatatcga aaacggcatt ggcgatatcg taaccaccgg tagcactaag tacttcaaga
     4261 ccaacaccga tggcgcagat gccaacgcgc agggtaaaga cagtgttgcg attggttctg
     4321 gttccattgc tgccgctgac aacagcgtcg cgctgggcac gggttccgta gcagacgaag
     4381 aaaatacgat ctctgtgggt tcttctacca accagcgccg tatcaccaac gttgctgccg
     4441 gtgttaatgc caccgatgcg gttaacgttt cgcaactgaa gtcttctgaa gcaggcggcg
     4501 ttcgctacga caccaaagct gatggctcta tcgactacag caacatcact ctcggtggtg
     4561 gtaacggcgg tacgactcgc atcagcaacg tctccgctgg cgtcaacaac aacgacgcag
     4621 tgaactatgc gcaactgaag caaagcgtgc aggaaacgaa gcaatacacc gatcagcgca
     4681 tggttgagat ggataacaaa ctgtccaaaa ccgaaagcaa gttgagcggt ggtatcgctt
     4741 ctgcaatggc gatgaccggt ctgccgcagg cttacacgcc gggtgccagc atggcctcta
     4801 ttggtggcgg tacttacaac ggtgaatcgg ctgttgcttt aggtgtgtcg atggtgagcg
     4861 ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt gaatactccg
     4921 ccgcactcgg tgccggtatt cagtggtaat gatccattaa cgaatgggta aacgccacat
     4981 tgcctgatgc gctacgctta tcaggcctac ggggtgattc agcacattcc tgtaggtcgg
     5041 ataaggcgtt cacgccgcat ccggcaagat aaggcactcc gggtcaacaa tctaagggca
     5101 attctctgat gaggattgcc cttttcttta ccagacatct cccaccacaa gaattggccc
     5161 taccaattct tcgcttatct gacctctggt tcacaattcc ccaattaaaa ctcacatcaa
     5221 tgttgccaac acgtaacatt tagttaacca ttcattgtca ttacccctac acaacacaat
     5281 tggcagtgcc acttttacac aacgtgtgac aaagagatgg gcaacagact cattacacgg
     5341 cgtgtgtgga ctgcaggaga cctgcaatga atctctggca acaaaactac gatcccgccg
     5401 ggaatatctg gctttccagc ctgatagcat cgcttcccat cctgtttttc ttctttgcgc
     5461 tgattaagct caaactgaaa ggatacgtcg ccgcctcatg gacggtggca atcgcccttg
     5521 ccgtggcttt gctgttctat aaaatgccgg tcgctaacgc gctggcctcg gtggtttatg
     5581 gcttcttcta cgggttgtgg cccatcgcgt ggatcattat tgcagcggtg ttcgtctata
     5641 agatctcggt gaaaaccggg cagtttgaca tcatccgctc gtctattctt tcgataaccc
     5701 ctgaccagcg cctgcaaatg ctgatcgtcg gtttctgttt cggcgctttc cttgaaggag
     5761 ccgcaggctt tggcgcaccg gtagcaatta ccgccgcatt gctggtcggc ctgggcttta
     5821 aaccgctgta cgccgccggg ctgtgcctga ttgttaacac cgcgccagtg gcatttggtg
     5881 cgatgggtat tccgattctg gtcgccgggc aggtaacagg catcgacagc tttgagattg
     5941 gtcagatggt ggggcgtcag ctgccgttta tgaccattat cgtgctgttc tggatcatgg
     6001 cgattatgga cggctggcgc ggtatcaaag aaacctggcc tgcggtcgtg gttgcgggcg
     6061 ggtcgtttgc catcgctcaa tacctcagct ctaactttat tgggccggaa ctgccagaca
     6121 ttatctcttc gctggtatcg ctgctctgtc tgacactgtt cctcaaacgc tggcaacccg
     6181 tgcgcgtatt ccgcttcggt gacttagggg cgtcacaggt tgatatgacg ctggctcaca
     6241 caggttacac ggcaggtcag gtgctgcgcg cctggacacc gttcctgttc ctgaccgcca
     6301 ccgtaacact gtggagtatc ccgccgttta aagccctgtt cacttcaggc ggtgcgctgt
     6361 atgagtgggt gatcaacatt ccggtgccgt acctcgataa actggttgcc cgtatgccgc
     6421 cagtggtcag cgaagcgaca gcgtatgccg ccgtgtttaa gttcgactgg ttctctgcca
     6481 ccggtaccgc cattctgttt gctgccctgc tctcgattgt ctggctgaag atgaaaccat
     6541 ctgacgctat cagcaccttc ggcagcacgc tgaaagaact ggctctgcct atctactcca
     6601 tcggcatggt gctggcgttc gcctttatct cgaactattc cggtttgtca tcaacactgg
     6661 cgctggcact ggcgcacacc ggtcatgcat tcaccttctt ctcgccgttc ctcggctggc
     6721 ttggggtatt cctgaccggg tcggatacgt catctaatgc tttgtttgcc gctttgcagg
     6781 ctaccgccgc acaacaaatt ggcgtttctg acctgttgct ggttgctgcc aacaccaccg
     6841 gtggtgtcac cggcaaaatg atctctccgc aatctatcgc tatcgcctgt gcggcggtag
     6901 gcctggtggg caaagagtct gatttgttcc gctttactgt caaacacagc ctgatcttca
     6961 cctgtatggt gggcgtgatc accacgcttc aggcctatgt cttaacgtgg atgattcctt
     7021 aatgattgtt ttacccagac gcctgtcaga cgaggttgcc gatcgtgtgc gggcgctgat
     7081 tgatgaaaaa aacctggaag cgggcatgaa gttgcccgct gaacgtcaac tggcgatgca
     7141 actcggcgtg tcgagaaatt cactgcgcga ggcgctggcg aaactggtaa gcgaaggtgt
     7201 gctgctcagt cgacgtggcg gcgggacgtt tattcgctgg cgtcatgaca catggtcgga
     7261 gcaaaacatc gtccagccgc tgaaaacgct gatggccgat gatccggatt acagtttcga
     7321 tattctggaa gcccgctacg ccattgaagc cagcaccgca tggcatgcag caatgcgcgc
     7381 cacacctggc gaaaaagaaa agattcagct ttgctttgaa gcaacgctaa gtgaagaccc
     7441 ggatctcgcc tcacaagcgg acgttcgttt tcatctggcg attgccgaag cttcacataa
     7501 cattgtgttg ctgcaaacca tgcgcggttt cttcgatgtc ctgcaatcct ctgtgaagca
     7561 tagccgccag cggatgtacc tggtaccccc ggttttttct cagctgaccg aacaacatca
     7621 ggcggtcatt gacgccattt ttgtcggtga tgctgacggg gcgcgtaaag caatgatggc
     7681 gcaccttagc tttgttcaca ccaccatgaa acgattcgat gaagatcagg ctcgccatgc
     7741 acgaattacc cgcctgcctg gtgaccataa tgagcattcg agggagaaaa acgcatgatt
     7801 atttccgcag ccagcgatta tcgcgccgca gcgcaacgca ttctgccgcc gttcctgttt
     7861 cactatatgg atgggggggc atattctgaa tacaccctac gccgcaacgt ggaagatttg
     7921 tcagaagtgg cgctgcgcca gcgtattctg aaaaacatgt ctgacttaag cctggaaacg
     7981 acgctgttta atgagaaatt gtcgatgccg gtggcgctag gtccggtagg tttgtgtggc
     8041 atgtatgcgc gacgcggcga agttcaggct gccaaagccg cggatgcgca tggcattccg
     8101 tttactctct cgacggtttc cgtttgcccg attgaagaag tcgctccagc tatcaaacgt
     8161 ccgatgtggt tccagcttta tgtgctgcgc gatcgcggct ttatgcgtaa cgcactggag
     8221 cgagcaaaag ccgcgggttg ttcgacgctg gttttcaccg tggatatgcc gacaccggga
     8281 gcgcgttatc gtgatgcgca ttctgggatg agcggcccga acgcggcaat gcgccgctat
     8341 ttgcaggcgg tgacgcatcc gcaatgggcg tgggatgtgg gcctgaacgg tcgtccgcat
     8401 gatttaggta atatctcggc ttacctcggc aaaccgaccg gactggaaga ttacatcggc
     8461 tggctgggga ataacttcga tccgtccatc tcatggaaag accttgagtg gatccgcgat
     8521 ttctgggatg gcccgatggt gatcaaaggg atcctcgacc cagaagatgc gcgcgatgca
     8581 gtacgttttg gtgctgatgg aattgtggtt tctaaccacg gtggccgcca gctggacggt
     8641 gtgctctctt ctgcccgtgc actgcctgct attgcagatg ccgtgaaagg tgatatcgcc
     8701 attctggcag atagcggaat acgtaacggg cttgatgtcg tgcgtatgat tgcgctcggt
     8761 gccgacaccg tactgctggg tcgtgctttc ctgtatgcgc tggcaacagc gggccaggcg
     8821 ggtgtagcta acctgctaaa tctgatcgaa aaagagatga aagtggcaat gacgctgact
     8881 ggcgcgaaat cgattagcga aattacgcaa gattcgctgg tgcaggggct gggtaaagag
     8941 ttgccggcgg cattggctcc gatggcgaaa gggaatgcag cttaagggtt agacgaatat
     9001 ctgctatcct gcccccgcac taagggggca gtatgctaaa catcgtactt tacgaaccag
     9061 aaattccgcc aaatactggc aacatcatcc gtctttgcgc taataccggc tttcgtctgc
     9121 atatcatcga accgatggga tttgcctggg acgataagcg cctgcgccgc gcggggctgg
     9181 actatcacga gtttaccgcc gttacgcgtc atcatgacta tcgcgcgttt ctcgaagcag
     9241 aaaatcccca gcgtctgttt gccctcacca cgaaaggtac gcctgctcac agcgccgtaa
     9301 gctatcagga tggcgattat ctgatgttcg gcccggaaac acgcggcctg ccagcgagca
     9361 ttcttgatgc cctgcccgct gaacaaaaaa ttcgtattcc aatggtgccg gacagccgca
     9421 gcatgaacct gtccaatgcg gtgtcggtcg tggtgtatga agcctggcgg cagttggggt
     9481 atccgggagc ggtattgaga gattagttac tgtaggccgg atagatgatt acatcgcatc
     9541 cggcacgatc acaggacatc agatcccatc accatactca aacgtatggt taatgccgtt
     9601 gaaatgctga tccatatcca ttgacggttt atcgctatcc ggcttgccga caatgcgtgc
     9661 cggaacgcca gcggcagtag tgtgcggcgg caccggttgc agcaccacgg aacctgcgcc
     9721 aatcttcgcg ccgcgcccaa cttcaatatt gccgagaatt ttcgcgcccg cgccaatcat
     9781 cacaccttca cgaattttcg ggtgacggtc gccaccagat ttacccgtac cgccaagcgt
     9841 cacagattgc agaatcgata cgtcgttttc aatcaccgcc gtttcaccaa cgacgatgcc
     9901 tgtcgcgtgg tcgagcatga taccgcgacc aatttttgct gccgggtgaa tatcaacctg
     9961 gaacgtcaca gaaacctggt tttgcagaaa aatagccagt gcgcgacgcc cctgattcca
    10021 caaccagtga ccgatgcgat acgcctgcaa agcatgaaaa cccttcaggt atagcaacgg
    10081 ggttgagtat ttatccaccg ccgggtcacg ggtacgcacc gcctgaatat cacaggccgc
    10141 agaggcgatc atttccgggt cggcggcgta ggcttcttcc accacttcac ggatagcaat
    10201 agccggcata atgggcgatg acagcttgtt cgccagcatg tagctcagtg cactgccaag
    10261 gttttcgtgc ttgagtagcg tcgcgtggta aaaactggcc agcattggct cacagtccgc
    10321 cagcgttctg gcttcagctt taatattgtt ccagacaatt tccagttctt cacacgacat
    10381 tgcttactcc acacgatgag ataatgaccg acccgttctg cgcgggccgg gtcatagtgg
    10441 taacaaaggt tccctgggtt tagtggctgc tgcgctcgtc cttgcgtgca cgacctagta
    10501 aagtcaatgc tgcctcgcgc gcgttttttc cgcaatataa tacttgataa atttcctcgg
    10561 ttattggcat ttcaacgccg aagcgatgcg ccagttcgcg gacttctttc gtattgcggt
    10621 agccttccac cacttgacca attttctcct gcgcgctttg tacatccatg ccctgaccga
    10681 gcatcatgcc aaaacggcgg ttacgcgact ggttgtcggt acaggtaagc accagatcgc
    10741 ccagccccgc catgcccata aaggtggcag gatcggcacc cagcgccgcg ccaagacgcg
    10801 acatctcagc cagcccacga gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac
    10861 cgtcggacat ccccgcgcca atggcaatga cgtttttcac cgcgccgcca agctgcacac
    10921 cgatgaaatc cgggttgctg taaacgcgga aacttttgcc acagtgcagc aattgctgga
    10981 ggtcatcggc aaaggtctgg tccgtcgagg ccagcgaaat ggccgtcggt aaacctgccg
    11041 ccagttcttt cgcaaacgtt gggccagaga taaccgccag cggaatttga tcgcctaagg
    11101 cctcacgcgc cacgtcctgt aacagacgtc cggtttctgc ttccagccct ttggttgccc
    11161 acaccagacg cgcatcagga cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga
    11221 catggctggg tacgacgacg agaatattac ggctggctgc cagcgcagtg gcgagatcgc
    11281 tttcaagatg gagcgtatcg ggaaaaggca catcggggag aaacgcggcg ttacagcggt
    11341 cgcgttcaag cgttgcgata tgttcagggt catggcccca gaggacaacc tcgtggccat
    11401 ttcttgccag ggtgatggca agagcggtgc cgtacgagcc ggcaccgatc acagtcattg
    11461 aagcattacg ttggttcatc aggcatcctg atgttcttca gtaccttcgc cagcctgctg
    11521 ctgcaaatag ttcatgaaca gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa
    11581 tgtaccgcgg gataccatgc tggtgatgca ctcacgagca tacgggaaca gaatgttcgg
    11641 gcagtatgcg cccaggcaat gtgccatctg ggtgccttcg atacccgcga tggagaaaat
    11701 accgccctgc tgaacttcac acaggaacgc ggtttcttcg cccagggagg ccgttacggt
    11761 aacacgcagt accacttcgt aaacgtcatc tgccagttga gtagatgccg tatccagatc
    11821 aagtttaact tctggttgcc aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa
    11881 agaaatatcc ttggtataaa tacgttggat ctggaaagtc atttcggtgt tgttttgttc
    11941 tgacatgtgt agaaaaccct taagtgttgt ccttaaaata ctgcgcaatg taatgccaac
    12001 gttcacacgt tatttcagca ggggatccag tccaccacgt gcatccaatg catacaagtc
    12061 atcacagcca ccaatgtgct gtgcgtcaat aaaaatctgt ggaaccgtgg tgcgaccgct
    12121 gcgtttgatc atctcttcac gcttagcggc attgccatcg atcggcaact cctgaaaact
    12181 cacgcccttg ctgcttagca gtgcttttgc acgatggcaa tacgggcagg tttctttggt
    12241 atagatttca acattggcca taaattagct cctgtttttt accctgaaat ttcatgttgc
    12301 agggcggcag caaaatggag tgtccccaga agcttatatc aataagtcgc tgggggccta
    12361 attgcagcta acggcctgca tcatgaaaga cgacaggtaa attatttgcc gcgcaccaaa
    12421 ggcaagtttt cgccagccca gccagcgacg ccttctttca gtacgaatac ttgtgcaaaa
    12481 ccagctttcg tcagcgcgtt tgcaggctcc tggcactgca tgccagaacc atctaccacg
    12541 ataaccggtt tgtctttgtg cttctcaagc tcaccaacat tgttggcttt gatttcgctc
    12601 ggcaacaggt tgatagaacc tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa
    12661 tccacaacca cagcgtcttc tttgttgatc agacgcgtag cttcaccacg agtaatcacc
    12721 ttcactttcg aggtcaggct cttaaacgta gtcacaagaa ccgccaccag taacgcgatc
    12781 caggcgatac tcagtatggg atgacggcca acaaattgca taatttcttg catggggggt
    12841 aacaactccc gacgtagtga ttaaaaaacc aggaaaggag tatacctgcg cagtgcggca
    12901 aatacagcca gcgcgttaac tggaatgcaa ttttgcgggg cgctacgaaa aaaaagcgta
    12961 aatctggcat ctgcttgccg cgagcggtcg tatattttga tcttcagagg ctattttatc
    13021 gattcagctg tagtaaaatt acgcaaattt tgactcttga gtatgaggtt gtcgcaatgt
    13081 cggtttctaa aaaacctatg gtactggtga ttctggatgg ctatggctat cgcgaagaac
    13141 agcaggataa cgccattttt agtgctaaaa ccccggtaat ggatgcactg tgggccaatc
    13201 gtccgcatac cctgatcgac gcttccggtc tggaagtcgg tctgcctgac cgtcagatgg
    13261 gtaactccga agtaggccac gttaacctgg gtgcaggccg catcgtgtat caggacctga
    13321 ctcgtctgga cgttgaaatc aaagatcgca ctttctttgc taatccggtg ttgactggtg
    13381 cggtagataa agcgaaaaac gcaggcaaag cagtacacat tatgggtctg ctctccgcag
    13441 gtggtgtaca cagccacgaa gatcacatca tggcgatggt agaactggca gctgaacgcg
    13501 gcgcagaaaa aatctaccta cacgcattcc ttgacggtcg cgacactccg ccgcgcagtg
    13561 ctgaatcctc gctgaaaaaa ttcgaagaga aatttgccgc gctgggtaaa ggccgcgtag
    13621 cgtccatcat tggtcgttac tacgcgatgg accgcgataa ccgttgggat cgcgttgaaa
    13681 aagcttatga cctgctgact ctggcgcaag gtgagttcca ggccgatacc gccgttgccg
    13741 gtttgcaggc tgcttatgct cgcgacgaaa acgacgagtt cgtgaaagcg accgttattc
    13801 gtgcagaagg tcagccagat gcggcaatgg aagacggcga tgcgctgatt ttcatgaact
    13861 tccgtgctga ccgcgcacgt gaaatcactc gtgcattcgt aaatgccgat tttgacggct
    13921 tcgcgcgtaa gaaagtggtt aacgtcgatt tcgtgatgct gaccgaatac gctgccgaca
    13981 tcaaaaccgc agtggcttac ccacccgctt ccctggttaa caccttcggc gagtggatgg
    14041 cgaaaaacga caaaactcag ctgcgtattt ccgaaaccga aaaatatgcc cacgttactt
    14101 tcttcttcaa cggtggcgta gaagagtcgt tcaaaggcga agatcgcatt ctgatcaact
    14161 caccgaaagt ggctacctac gatctgcaac cggaaatgag ctccgcagag ctgaccgaaa
    14221 aactggttgc ggcaatcaag agcggcaaat acgacaccat tatctgtaac tatccgaacg
    14281 gcgacatggt aggtcacacc ggggtgatgg aagcggcggt taaagcggtt gaagcgctgg
    14341 atcactgcgt ggaagaagtc gcgaaagcgg ttgagtccgt gggtggacaa ctgctgatca
    14401 ccgctgacca cggtaacgct gaacagatgc gcgatccggc aacgggtcag gcacacacag
    14461 cacacaccaa cctgccagtt ccgctgattt acgttggtga taagaacgtg aaagcggttg
    14521 aaggcggcaa actttctgac atcgcgccga ccatgttgtc gctgatgggt atggaaatcc
    14581 cgcaagagat gactggtaag ccgctgttca tcgtggaata atccctcccc atgaggggaa
    14641 aggcgattaa taccatgaca cgggccgtga aaccgcgcag gtttgcaatc aggcccatca
    14701 tctacgccag cgttctgagc gctggcgtat tgttgtgcgc cttttccgcc cacgcggatg
    14761 agcgtgacca actcaaatcc attcaggctg acatcgccgc gaaagagcgc gcggtacgcc
    14821 aaaagcaaca acaacgcgca agcctgctcg cacaattgaa aaagcaggaa gaagcgatct
    14881 ctgaagccac ccgtaaactg cgcgaaacgc aaaacacgct taatcaactg aataaacaga
    14941 ttgatgagat gaacgcgtcg attgccaaac tggagcagca aaaagccgcc caggagcgca
    15001 gcctcgccgc gcaactggat gccgcgtttc gtcagggtga acataccggt attcagctga
    15061 ttctcagcgg tgaagaaagc cagcgtgggc agcggttaca ggcttatttc ggctatctca
    15121 accaggcgcg acaagaaacc attgctcagt tgaaacaaac gcgtgaagaa gtcgctatgc
    15181 agcgtgccga actggaagag aaacaaagcg agcaacaaac gcttttatat gagcagcgcg
    15241 cccaacaggc gaagctgacc caggcgttga gcgagcgtaa aaagacgctg gcagggctgg
    15301 agtcttccat ccagcaaggt cagcaacagt tgagcgagct gcgcgccaac gaatcccgcc
    15361 tgcgtaacag cattgcccgt gcggaagccg cggcgaaagc gcgtgctgaa cgtgaagcgc
    15421 gcgaagccca ggcggttcgc gaccgccaga aagaagcgac gcgcaaaggc accacctaca
    15481 agccgaccga aagcgaaaaa tcgctgatgt cccgtaccgg tggtctgggc gcaccgcgcg
    15541 gtcaggcatt ctggccggtt cgcgggccaa cgctgcatcg ctatggcgag cagctacagg
    15601 gtgaattacg ctggaaaggg atggtgattg gtgcctctga aggtactgaa gttaaagcga
    15661 ttgccgacgg ccgggtgatt ctggctgact ggctgcaagg ctacggtctg gtggtggtgg
    15721 ttgagcatgg taaaggcgac atgagtcttt acggctataa tcagagcgca ctggtgagcg
    15781 ttggttcgca ggttcgcgcg ggccagccaa ttgcactggt gggcagcagt ggcggtcagg
    15841 gtcggccttc actctatttc gaaattcgcc gccagggtca ggcggtcaat ccacagccgt
    15901 ggttgggaag ataagttttg tttccatttc gtcgtaacgt tcttgcattt gccgctctgt
    15961 tggcgctctc ctcccccgta attgctggca aacttgccat cgtcattgat gattttgggt
    16021 atcgcccgca caacgaaaac caggtgctgg cgatgccttc cgctatctcc gtcgctgtat
    16081 tacccgattc accgcacgcc agagagatgg cgaccaaagc acataacagc ggtcacgaag
    16141 tgttgattca tctaccgatg gcaccactga gtaaacagcc gctggagaaa aatacgctac
    16201 gcccggagat gagcagcgac gaaattgagc gcattattcg tagtgcggtc aataacgtgc
    16261 cctatgccgt ggggatcaac aaccacatgg gcagcaagat gacctctaac ctgtttggta
    16321 tgcagaaagt gatgcaggcg ctggagcgtt acaatcttta ctttctcgac agcgtaacca
    16381 tcggtaatac ccaggcgatg cgcgccgcgc aaggcactgg cgtgaaggtg atcaaacgga
    16441 aggtgttcct cgacgattcg cagaacgaag cggacatccg tgtgcaattc aatcgcgcaa
    16501 ttgacctagc gcgtcgcaac ggttcaacca ttgccattgg gcatcctcac ccttcaacgg
    16561 tacgcgtgct acaacagctg gtttataacc tgccgccaga cattacgctg gtgaaagcca
    16621 gcagcttgct gaatgaaccg caggttgata cgtctacacc gccgaaaaac actgtgcccg
    16681 acacaccgcg taatccgttc cgtggcgtga agctgtgcaa accgaagaag ccgctggaac
    16741 ctgtttatgc taatcgcttc tttgaagtat taagcgaaag catcagccag agcacgctga
    16801 tcgtttactt ccagcatcag tggcaaggct ggggcaaaca gcccgaagcg gcaaaactta
    16861 acgctagcgc aaattaaagg cgctacgcgt aatgcgatgg tgcgaccgcg ttttgtcgcg
    16921 ccattgccat aagcggaaag accacagtaa cgcctggtaa cccaccttca cgctgcggac
    16981 attggtaatc aggcgcttat acataccgga agtaaatatc tcggcaatca tccgttggcg
    17041 ggtaagaata tccggctctt tgcgcaccgc atggcaaaca cgcaatgctt cgtaagttat
    17101 ttgctgatga aattccggat aaatcgtaat tttgtcagca taatttcgat ttaatttctc
    17161 cagcaggcgg gtaatcttaa tatagtgacg ttgataatta aggtttttat tcccttgtct
    17221 atgcaaccga ctcactgacg tattatgcag ataatattta tataatgatt gctcggtata
    17281 tcgcgcacgc agcgcgttaa acataaattc tgttgtccag acaatatcct gatgatgtaa
    17341 tccggcaata aatttaatgt tatttttaac aataacatcg cggcgataaa cccccatcca
    17401 gaccacgtgt gtccaacggc gggaagaaag tcccatccgc agccagtccg gaccggttaa
    17461 tacaccggtt gagcgaaggc gatcggtggg gatggattgc caggtttcac ccgtttcacg
    17521 aaaacaccaa tcagcgttgc actgcgccac gtcgaggtca tcctctaacg ccatggtcat
    17581 cagcgtttcg tacatggtgg gatagacctc atcgtcagca tcgacaaaag cgacatattt
    17641 tcccgttgcc acttcaatcc cacgattacg cgccaccgat gcgccagcat tcgcctgatg
    17701 caacaaacga acgtgcggat agttttctgc gtaatgcttt gctatttcaa cagaattatc
    17761 cgttgaacca tcgttaataa taatgatttc cagagcagtc catgtttgcg taattaaaga
    17821 ttccatacaa gtgcggaaat catcgcccgc attatataac gggataataa cactaagttt
    17881 attggtgctg ttcatcataa attaccgacg acctctgaag ttatcctgac gttttacagg
    17941 ctttgtatta agaaactatt aagcctgtga gatattattt ttaccggtga gagagtcact
    18001 tcagggtaga gagataatga gagcagctat ataagagatc agtgcaatta cgaactcttt
    18061 attacactga caggatttta catatttaac ggattatcct caggcaaaag ggctggaata
    18121 ccagcccttg ttcgtgttaa tcccagctca gaataacttt cccggactgg cccgaacgca
    18181 tagcgtcaaa gcccttctgg aaatcatcga tagagaaacg atgggtaatg atcggcgaga
    18241 gatcgaggcc agactgaatc agcgccgcca tcttgtacca ggtttcaaac atctcacgac
    18301 cgtaaatacc tttaatgaac aagcctttaa agattacttt ggtccagtcg atagacatat
    18361 cggacggtgg aatacccagc atcgcaatac gaccgccgtg gttcatggtg tcgagcatgg
    18421 tacgaaacgc tggcggcgca ccggacattt ccagaccgac atcaaagcct tcggtcatgc
    18481 ccagttctgc catcacatca ttaaggtttt ctttagcgac gttaaccgca cgggtgatac
    18541 ccattttgcg tgccagctca aggcggtatt cgttaacatc agtgatcacc acattgcgtg
    18601 caccaacgtg tttcgccacc gccgcagcca taatacctat cggacctgca ccagagacca
    18661 gcacatcctc gccaaccaga tcgaacgaca gcgcggtatg cacggcgtta ccgaagggat
    18721 caaaaatgga agccaggtcg tcggagatat tgtctgggat tttgaaggcg ttgaacgccg
    18781 gaatcaccag atactcggcg aagcagcccg gacggttaac accgacgccg atcgtgttgc
    18841 ggcacagatg ggtacgaccg ccacgacagt tgcgacagtg accgcaggtg atatggcctt
    18901 cgccagaaac gcgatcgcca atcttgaagc ctttcacttc ctgaccaata cctaccactt
    18961 caccgacata ttcatggccc acgaccatcg gcaccgggat ggttttttgc gaccactcat
    19021 cccagttata gatgtgaacg tcagtcccgc agatggctgt tttacggatt ttaatcagca
    19081 gatcgttatg cccgagttcc ggtacaggaa cgtcggtcat ccagatgccc tcttccgctt
    19141 tcagtttgga taacgctttc atctcacacc ctcaggcgat aacgcccagt tgtttaccaa
    19201 tacgcgtgaa tgcttctact gcacgcgtaa tttgctcagg ggtatgcgcc gcagacatct
    19261 gggtacgaat acgcgcctga cctttaggaa cgaccggata gaagaaaccg gtgacgtaaa
    19321 tgccctcttt ttgcagttca cgggcaaatt tttgcgccac tacagcatcg ccaagcatga
    19381 ccggaataat ggcgtgatcg gcacccgcca gggtaaagcc cgccgccgac atctgctcac
    19441 ggaactgacg cgcgttcgcc cacagacggt cacgaagctc actgcccgct tcgaccatct
    19501 ccagcacttt aatggaagca gccacaatgg caggtgccag tgagttggag aacaggtaag
    19561 gacgagaacg ctgacgcagc cactcaacca cttctttacg cgctgcggtg taaccaccag
    19621 aagccccgcc caacgcttta ccaagcgtac cggtgataat atcgacccgc cccatcacat
    19681 cgcagtattc atgggaacca cgaccatttt caccgacaaa accgaccgcg tgggagtcgt
    19741 ctaccatcac cagggcatcg tatttatctg ccagatcgca aacgcccttc aggttggcaa
    19801 tcacgccgtc catcgagaac acaccatcgg tggcaatcag cacatgacgc gcaccggctt
    19861 cacgcgcttc tttcagacga gcttccagct catgcatatc gttgttggca taacgatagc
    19921 gtttagcttt acacagacgc acaccatcaa taatagacgc gtggttcagt gcgtcggaga
    19981 taatggcgtc ctccgcgctc agaagcgttt caaacaggcc gccgttagca tcaaagcagg
    20041 aagagtagag aatcgcatct tccatcccca ggaatgccgc cagtttttgt tcaagctctt
    20101 tatggctatc ctgggtgccg cagataaagc gcaccgaagc catcccaaaa ccatgagagt
    20161 ccattcccgc ttttgccgcc gcaatcagct ctggatgatt tgccagtccg agatagttat
    20221 tggcgcaaaa gttaataacg tggcttccat cagccacagt gatatctgct tgctgcgcag
    20281 acgtaataat gcgctcttct ttaaacaacc cttccgcccg tgcggtttcc agatcgttgg
    20341 ttaactgctg ataaaattct ccacgcattg cgattctcca gacagggcaa attccagcac
    20401 atattaccca aacttctagg tcgcgacgag atgacgcgtt aacacttctg caaaattcag
    20461 gataaataac gtccatcaat ctcacttttg gcgaatggct gaagccaacg ccgtgtagtg
    20521 atatgataga agtattcgtg tctgagattg tctctgactc cataattcga aggttacagt
    20581 tatgatcatc gttaccggcg gcgcgggctt tatcggcagc aacatcgtta aggccctgaa
    20641 tgataaaggc atcaccgata ttctggtggt ggacaacctg aaagacggca ccaagtttgt
    20701 gaacctggtg gatctggata tcgcggacta tatggataag gaagacttcc tgatccagat
    20761 tatggctggc gaagagttcg gcgatgtcga agcgattttc cacgaaggcg cgtgctcttc
    20821 caccaccgag tgggacggca agtatatgat ggataacaac tatcaatact ccaaagagct
    20881 gctgcactac tgtctggagc gcgaaatccc gttcctgtat gcctcttccg cagccactta
    20941 cggtggacgc acctccgact ttattgaatc ccgcgaatat gaaaaaccgt tgaatgtcta
    21001 cggttactca aaattcctgt ttgatgaata tgttcgccaa atcctgcctg aagcgaactc
    21061 gcagattgtt ggcttccgtt atttcaacgt ttatggacct cgtgaaggcc ataaaggcag
    21121 tatggcgagc gtcgctttcc atctcaacac ccagcttaat aacggtgaat caccgaagct
    21181 gttcgaaggt agcgagaact ttaaacgtga cttcgtctat gtgggcgacg tggcagatgt
    21241 aaacctgtgg ttcctggaaa atggcgtttc cggcatcttc aacctcggca ccggtcgtgc
    21301 ggaatccttc caggcagtag cagatgctac gcttgcttat cacaagaaag gccaaatcga
    21361 atacattccg ttcccggata aactgaaagg ccgctaccag gcgttcactc aggcagatct
    21421 gacgaatctg cgcgcggcgg gttacgataa accgttcaaa accgttgccg aaggcgtaac
    21481 ggaatacatg gcctggctga atcgtgacgc ataagagctc tgcatgaaaa tactggtgat
    21541 cggcccgtct tgggttggcg acatgatgat gtcgcaaagt ctctatcgca cgctccaggc
    21601 gcgctatccc caggcgataa tcgacgtgat ggcaccggca tggtgccgtc cattattatc
    21661 gcggatgccg gaagttaacg aagctatccc tatgcctctc ggtcacggag cgctggaaat
    21721 cggcgaacgc cgcaaactgg gtcatagcct gcgtgaaaag cgctacgacc gcgcctacgt
    21781 cttacctaac tccttcaaat ccgcattagt gcctttcttc gcaggtattc ctcatcgcac
    21841 cggctggcgc ggcgagatgc gctacggttt actcaacgat gtacgcgtgc tcgataaaga
    21901 agcctggccg ctaatggtgg aacgctatgt cgcgctggcc tatgacaaag gcattatgcg
    21961 tacagcacaa gatctgccgc agccattgtt atggccgcag ttgcaggtga gcgaaggtga
    22021 aaaatcatat acctgtaatc aattttcgct ctcatcagaa cgtccgatga ttggtttttg
    22081 cccgggtgcg gagtttggtc cggcaaaacg ctggccacac taccactatg cggagctggc
    22141 aaagcagctg attgatgaag gttatcaggt ggttctgttt ggctcggcga aagatcatga
    22201 agcgggcaat gagattcttg ccgctttgaa taccgagcag caggcatggt gtcggaacct
    22261 ggcgggggaa acacagcttg atcaagcggt tatcctgatt gcagcctgta aagccattgt
    22321 tactaacgat tctggcctga tgcacgttgc ggcggcgctc aatcgtccgc tggttgccct
    22381 gtatggtccg agtagcccgg acttcacacc accgctatcc cataaagcac gcgtgatccg
    22441 cctgattacc ggctatcaca aagtgcgtaa aggtgacgcg gcggagggtt atcaccagag
    22501 cttgatcgac attactcccc agcgcgtact ggaagaactc aacgcgctat tgttacaaga
    22561 ggaagcctga cggatgcggg ttctgatcgt taaaacatcg tcgatgggcg atgttctcca
    22621 tacgctgccc gcactcactg atgcccagca ggcaatccca gggattaagt ttgactgggt
    22681 ggtggaagaa gggttcgcac agattccttc ctggcacgct gccgttgagc gagttattcc
    22741 tgtggcaata cgtcgctggc gtaaagcctg gttctcggcc cccataaaag ctgaacgcaa
    22801 agcgtttcgt gaagcgctac aagcagagaa ctatgacgca gttatcgacg ctcaggggct
    22861 ggtaaaaagc gcgacgctgg tgacgcgtct ggcgcatggc gtaaagcatg gaatggactg
    22921 gcaaaccgct cgcgaaccgt tagccagcct gttttacaat cgtaaacatc atattgcaaa
    22981 acagcagcac gccgtagaac gcacccgcga actgtttgcc aaaagtctgg gctatagcaa
    23041 accacaaacc cagggcgatt atgctatcgc acagcatttc ctgacgaacc tgcctacaga
    23101 tgctggcgaa tatgccgtat ttcttcatgc aacaacccga gatgataaac actggccgga
    23161 agaacactgg cgagaattga ttggtttact ggctgattca ggaatacgga ttaaacttcc
    23221 gtggggcgcg ccgcatgagg aagaacgggc gaaacgactg gcggaaggat ttgcttatgt
    23281 tgaagtattg ccgaagatga gtctggaagg cgttgcccgc gtactggccg gggctaaatt
    23341 tgtagtgtcg gtggatacgg ggttaagcca tttaacggcg gcactggata gacccaatat
    23401 cacggtttat ggacctaccg atccgggatt aattggtggg tatgggaaga atcagatggt
    23461 ttgtagggct ccaagagaaa atttaattaa
//
LOCUS       DACADF010000073        23377 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.073, whole genome shotgun sequence.
ACCESSION   DACADF010000073 DACADF010000000
VERSION     DACADF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.073"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..369
                     /locus_tag="ISB30_002690"
     CDS             <1..369
                     /locus_tag="ISB30_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1990306.1"
                     /translation="IKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISP
                     INYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFK
                     NCFAEQEILSEFPQPVSLAG"
     gene            complement(412..1563)
                     /locus_tag="ISB30_002691"
     CDS             complement(412..1563)
                     /locus_tag="ISB30_002691"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAP1990307.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1577..3172)
                     /locus_tag="ISB30_002692"
     CDS             complement(1577..3172)
                     /locus_tag="ISB30_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase YdiF"
                     /protein_id="HAP1990308.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3319..4077)
                     /gene="aroD"
                     /locus_tag="ISB30_002693"
     CDS             complement(3319..4077)
                     /gene="aroD"
                     /locus_tag="ISB30_002693"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP1990309.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4108..4974)
                     /gene="ydiB"
                     /locus_tag="ISB30_002694"
     CDS             complement(4108..4974)
                     /gene="ydiB"
                     /locus_tag="ISB30_002694"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAP1990310.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMEPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4986..6251)
                     /locus_tag="ISB30_002695"
     CDS             complement(4986..6251)
                     /locus_tag="ISB30_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1990311.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(6478..7692)
                     /locus_tag="ISB30_002696"
     CDS             complement(6478..7692)
                     /locus_tag="ISB30_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1990312.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHTLKENIAPGG"
     gene            complement(7792..8148)
                     /locus_tag="ISB30_002697"
     CDS             complement(7792..8148)
                     /locus_tag="ISB30_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HAP1990313.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGNSESWRQWENGKCAIP
                     DYVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8284..8391)
                     /gene="rprA"
                     /locus_tag="ISB30_002698"
     ncRNA           complement(8284..8391)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ISB30_002698"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8577..9689)
                     /gene="ydiK"
                     /locus_tag="ISB30_002699"
     CDS             complement(8577..9689)
                     /gene="ydiK"
                     /locus_tag="ISB30_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAP1990314.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            9903..12959
                     /locus_tag="ISB30_002700"
     CDS             9903..12959
                     /locus_tag="ISB30_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP1990315.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            12956..13366
                     /gene="menI"
                     /locus_tag="ISB30_002701"
     CDS             12956..13366
                     /gene="menI"
                     /locus_tag="ISB30_002701"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAP1990316.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSVREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13466..13654
                     /locus_tag="ISB30_002702"
     CDS             13466..13654
                     /locus_tag="ISB30_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990317.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14203..14571
                     /gene="sufA"
                     /locus_tag="ISB30_002703"
     CDS             14203..14571
                     /gene="sufA"
                     /locus_tag="ISB30_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAP1990318.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14580..16067
                     /gene="sufB"
                     /locus_tag="ISB30_002704"
     CDS             14580..16067
                     /gene="sufB"
                     /locus_tag="ISB30_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP1990319.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTVISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16077..16823
                     /gene="sufC"
                     /locus_tag="ISB30_002705"
     CDS             16077..16823
                     /gene="sufC"
                     /locus_tag="ISB30_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP1990320.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16798..18069
                     /gene="sufD"
                     /locus_tag="ISB30_002706"
     CDS             16798..18069
                     /gene="sufD"
                     /locus_tag="ISB30_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP1990321.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVCIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPTKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTVRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALCDEGLKQQVMARIGQRLPGGAR"
     gene            18066..19286
                     /gene="sufS"
                     /locus_tag="ISB30_002707"
     CDS             18066..19286
                     /gene="sufS"
                     /locus_tag="ISB30_002707"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAP1990322.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGVLYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALAQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19299..19715
                     /gene="sufE"
                     /locus_tag="ISB30_002708"
     CDS             19299..19715
                     /gene="sufE"
                     /locus_tag="ISB30_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAP1990323.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19863..20867
                     /gene="ldtE"
                     /locus_tag="ISB30_002709"
     CDS             19863..20867
                     /gene="ldtE"
                     /locus_tag="ISB30_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAP1990324.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(20930..21166)
                     /locus_tag="ISB30_002710"
     CDS             complement(20930..21166)
                     /locus_tag="ISB30_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAP1990325.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(21477..22889)
                     /locus_tag="ISB30_002711"
     CDS             complement(21477..22889)
                     /locus_tag="ISB30_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase I"
                     /protein_id="HAP1990326.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         6067 a         5625 c         5891 g         5794 t
ORIGIN      
        1 ataaaagata ttcttttcta tctgaataat aactatcgcg aaaaaatcac tttagagcag
       61 ttatcgaaaa aatttcgtgc cagcgttagt tatatctgcc atgaatttac caaagagtat
      121 cgtatttccc ctattaacta tgttattcaa cggcgtatga cggaagcgaa atggtcactc
      181 actaatactg aattatcaca ggcagagatc tcctggcgtg tgggttatga aaatgtcgat
      241 cactttgcca aactgttttt gcgccatgtc ggctgttcgc ccagcgatta ccgcaggcaa
      301 tttaaaaact gttttgcgga acaagaaatc ctatctgaat ttcctcaacc agtaagtctt
      361 gccggataaa tctggcggcg cagtttgtta agaagctgcg ccgcctgcag attatttgtt
      421 ctgataatct ttcaggatct gccgacctgc tacgtaaatc ataatttcgt ctgtaccgcc
      481 gccgatacgt tcacaacgga catcacgcca gaagcgggag acgcgcgcct catcggtata
      541 gcccagaccg cccatgattt gaatcgcatc atcaatgact tccattgcgg tacgtgcgca
      601 gtacagtttt gccagcgccg cgctggtgcg cagtgactga tgctgatcgg cttgccatgc
      661 cactttcaac accatgttgc gcatgttgtc gatcttaatc gccatcagcg ccagtttttc
      721 ctggatcatc tggttatgac caatgggctt accaaaagcg atacgctggt tggcatagcg
      781 ggcggcatct tcgaatgcgc attcggcaaa gccggtgctg cgcgcggcgt tgatcaggcg
      841 ctccatctca aagttgtaca tcacattgag gaaacccatt ccttcttcgc ccaccatgtc
      901 gctctcttca acttccacgt tgtcgagata gacttcgcag gtgctgagca tatgccagcc
      961 gattttatgc agtggattga ttttaatgcc gggcttactg gagtcgaccc accacagagt
     1021 aaaggccttt ttcggatctt tcggttgcgg atcgcgcgcc aacaccagca tatatggata
     1081 ctctttcgca ccggtaataa aggttttctg tccgttgatg taaaccttgc cgtttttacg
     1141 cgtgtaagtg gtagtggcac tgttgttgtc tgagcctgcg cctggctccg tcaatgccag
     1201 ggcataggcg ggatcacccg tttccagggt gctctctgcc gttttacgta gctgctctgc
     1261 agaaccgaaa cggcgcatac tgtgaataca ttgaccgttg gtaatcaaaa atgccggagc
     1321 accgcatttt gacacttcca tcagcgccag catttgggtg acgtaatccg cagggatacc
     1381 accaaattct tccggcacgc caagcatgga aataccgtta tccgccagtg cccgcataaa
     1441 ctcacgcggg tatgtcccgt tttgatcgca ggtgcggaaa tactcttccg gaaagttagt
     1501 cgtaatcagt tcgcgaatac tggccagcag cagttcttgt tcttcagtta aagagaaatc
     1561 catcattata ctcctgttaa tgagccgctt caggcaggac aaaacccatc gccgcatcga
     1621 taaataatct ttcgtccatc agtttgagtt ctggcgaaat cactggggtg aaatccattt
     1681 tgtcgagaat atctttttgt aaatcgacgc caggagcgat ttcgattaaa tgcaggccgt
     1741 cttcttttag cgtgaatacg gcgcgctcag tgatataacg aacatccagc cctcgctcga
     1801 gagcgatttt tccgctgaaa gttatttccg gtagttcccg aataaatttc ttcacccgtc
     1861 cttcctggac gatatttaat ttgccgtcgg taatttctgt ttttaaactg cccgcagtta
     1921 atgtgccgca gaaaacgatt ttcttcgaag tggcactgat atcaataaat ccaccggtgc
     1981 ccatgatttt accattgaat ttatgcacgc cgacgttacc gtgctggtcg acttcagcaa
     2041 aactcaaata acaaacatcc agaccaccac cgtgataaaa atcaaactgg gacgtcatat
     2101 ccagaatggc gcgggtattc acgttcgcgc caaaggcgat cccctgtgaa gtaataccgc
     2161 caatcggacc tgtttctacc gtcagaataa agtcatcagc acaaccttct tcccgggcga
     2221 ccaggccaat gccgtcagca ataccgacgc cgacattccc cactgcgcct ttgcgcattt
     2281 cgaataatgc acgccgtgca actaatttac gttggtttag gggcaggcta agtttggtgc
     2341 tgtcatcaag ggtgaagtca ccagaaataa agcggttaac cggcgcaccg ccatacagtt
     2401 gggtttgatc cggatcgacc accacaatat ccaccagata acccggaata cggacagatt
     2461 taggatgcag cgtggctttc ttaaccattt tctgcacctg catcatcaca ataccgccgt
     2521 tattgtgcac cgcctgggca ataaccaatg cgtcgagata catcacctca tcttcaaaag
     2581 tggcgtagcc ttcgctgtcg caggtggtag cgcgaatgaa ggcgatatct ggcgcaatcg
     2641 ctttgtaata gagatattct ttgttatcaa actcgaccag tttaatcagg tcttctttag
     2701 tgacttcatt cagtttgccg ccttgctggc gtggatcgac aaatgttccg atgccaatat
     2761 cactaataat accaggctgg tgggctgcgg cggcgcgtaa ggtttgtgta agtacacctt
     2821 gtggatagtt ataagcaata attttatttt gttctgcgag atcagaaata cgcggtgatt
     2881 gtccccagtg accgcataat gcccatttca ccaggccttc ttgcgccagc ggactaatac
     2941 cacggtcggc gcgatcgcca agccctgttg gactaataat cgataaatta cgtggtgttt
     3001 gagtctgttt atatttatca gcaagagcag taattaacgt ggtggcttcc agaataccgc
     3061 cgccagcgcc taacacacaa agtgttgctt cgtcgggaat ataattcacc gcttcctgtg
     3121 ccgataggac cggcactcgt ccattaatac gaggtggttt tacaggtttc attgttaatt
     3181 cctgattcag gtgtgatttt ccctacgcca gatggagaat tagcgcacag agactcacga
     3241 aatattttta ttaaatacgg ttaaaaatac tttttttagt tcggcgggag agggtattcc
     3301 cgccgaaaat attattgctt atgcctgatg taaaatagtt aataccgtac gcaaatcatt
     3361 taccgagatt tgccctggcg cagaggcttt ttttaccgca ccaaaagttg ccgccgaccc
     3421 aaatacttca ccagccagac gagaaattac gccagttttt gccatcgaca tcgtgatgat
     3481 tggacgatcg gcatactgct cctgcatctc cagggtcgcg gcaagcaacg tcagcacatc
     3541 gctggtactt tgcggcatca gcgcaatctt aggaatatcg gcgtcgaagg actgcatttt
     3601 gcgcagacgg gcaatgattt cttcggcttc cggcgtttta tggaagtcat ggttggacat
     3661 gacaactttc acatcatgcg cgtgggcgta ggcgacggtt tctttaacct gatcatcgcc
     3721 ggtaaataac tccagatcga tcatatcaac caggccgctg tcgatggctg cacgattgag
     3781 tgcaatataa gcctcggtgg aaatcgcctg ctcgccgcct tctttggcac tgcggaaggt
     3841 aaacagcagc ggtttttctg gcatggtttc acggagaatt tttgctgccg ccatgacaga
     3901 ctccacattg gagaggtcgg catagtggtc cacacgccat tccagaatat caaagtccgc
     3961 ttcacgatag gcgagagctt cggatttcac gctggcgata tctttcgcca tcagcgagac
     4021 gatgatttta ggtgcgcccg taccaatgac gagatctttt acagttacgg ttttcatttt
     4081 ttaccctttc tgcacgcggt cagcctgtca ggcaccgaac cccatgacct gtttaacata
     4141 ttccagaggg aaatctttgc cagtccataa cgtgaactgt tcagcccctt gccacaacaa
     4201 catgccgtat ccatcaatcg ttttgcaacc cgcttgttgc gcctgctgca ataacttcgt
     4261 catatgaggg ttatacacgc attcagtgac cagaagtccc gggtgtaaca gactgatatc
     4321 attaaccaat gattcattct caaggggttc catacccact ttggtgccat tggttaaaat
     4381 gtcggcggaa gccagggctt ctgcaaaggc ttgctgatcg gcgagatcgg tgactgtcac
     4441 gacgcaatcg gtgttttcgt taacccgctg cgcgaaggcg agagctttat cgaagaactc
     4501 atcccgacgg ttaaagagtt taatttcttt taaaccttca attgccccct gcgcgccaat
     4561 tgccgttgag gcaccaccgg cccctaatag caccatcgtt ttgcctttga tatcaaaacc
     4621 gctctcttta atggcgcgaa tatgacccgt gccgtcggtg ttatagccac gcagatagcc
     4681 atcatcattt acgatggtgt tgatggcacc caccagtttg gccgctggcg ttaattcatc
     4741 aacatattca cacgccagtt gtttgttcgg cattgataca ccagttccgc gcattttgag
     4801 ggcttttaat ccttcaattg ctccgggaaa actatcgtta tccacttcaa aggccatata
     4861 ggtaaatggc aatcccgctt tttctaaggc tttattctgc atttcgggcg ataaactgtg
     4921 gcggatggga taggccatca acccaatcaa ttcgtatttt gcggtaacat ccataattaa
     4981 ctcctttaac ctctatgctt aattgtgttt aaccgggtgc taaatttatg ctcgccaaac
     5041 cgcacatcat tttcaggaat aatgaaaacg cggtaatagc ggataaaaac aataattgcg
     5101 gtaatcaggg ccagcagcgc gaaagtaaaa tcgagaacaa tgatatattg caggccgatg
     5161 ttcgacagat aaccggtaat cagcggaata acaaagttag ccagtccacc catcatcata
     5221 tatatactgg tgactttggc tttgcttttt gggaaaaact ctgacatgac cgaaacgccg
     5281 agctgtaaaa tgccgccagc tgctgagaaa ccgataacaa aggctccggc attacacacc
     5341 aatggagatg ggtacaggta gataatggca gctgttattg ttgccagtgc agagttaaat
     5401 acattagccc agatgggccg gaccattttt ttcagcagtg cggcaaaaat aaagacacag
     5461 accagcgaac ccatactgta ataagagatg gtttttaatg cctcagcttc tgacatacca
     5521 gcaaaagcca tcgcatattt gggcatccac accacaatca cataaaaggt cgagaatgcg
     5581 gctacaccga acagtactga tgaaacacct tccagccaga ctaacggttt gctgttcatt
     5641 tgcggtaatt cattagctac gctggcgtcc accaactggc tggggaattt gcttttcaac
     5701 agcatcagcg tgatcagtac aaacagaata cccggaataa tcagcccata gccgtaccag
     5761 atgttattga gcaacatata gctcaccagc attggataga acatttgccc gaatgacacc
     5821 atcgctttaa ccagtatgac cgccgaacca gaggctttcg gaaagcattc catgagcgcg
     5881 gggtagccac ccgtatccag cgctgagtta gcgataccta cgcacactgc cagacagtag
     5941 gcgagagtta aattcgggca agcaggaata ccaaagaaga atagcagata cattattact
     6001 gccattaata tcaccgcccg acgaccaaac ttatcggaga tcactccgaa gaataaaata
     6061 ctgatcaatc gccccaatcc gataccggaa attaaatagg caatgcccgc gttgtcagtg
     6121 gaaaactttt ctgccaaaga tgtcatgttt tgggcaagcg taataacact aatgccgtgc
     6181 aggaaatagc tgaagtaaat acaaagaaca gccaggataa atggcgtgct gaaagcctta
     6241 ttttgagaca tgttcacagc tccttgctga aagataattt cgttaccgta cgtattgttc
     6301 tccgacggtt tacataatta ctatactgaa gatgattttc cctgggtaca tggcataaag
     6361 tttatgtttc caggaatatt gcatgtcata gtttgttttt gatcgctatt ttccttagtt
     6421 agcgcagaaa gagttattta tccgtaagat cttgcataac ctgaaaagaa tattgcatta
     6481 cccacccgga gcgatatttt cctttagcgt gtgatgccgc gtttctttgc gactgcgtaa
     6541 gccaataaac agtgccagta taaagccgat agcagcgatg gcggtatcga accacataat
     6601 atcggcaata cttctttgcg aaagatgagc cgtaatcaac ggaatggtaa atgttgcaat
     6661 gctgcccgca ctgtaataga tccccgtagc tttaccttta gcgtagggaa aacgttccgc
     6721 cattaacgtc aggccaattt gtacaacacc gcctgcggag gtaaaaccga tgacgaaagc
     6781 aaatattatc accacataaa atgtgggatg caggcagacg gtgagcaggg cgataaatga
     6841 gataaaggtg tacagcatca gtaatgttgt tgggcgaacg gtattacgaa tgagtggagc
     6901 agtaataaat acacacagca gcgaacccac ggtgtagata ctgagtaatt tgatcgacat
     6961 ggtgtatgac atgccagcga caaattgtcc gtattgtgcc agccactggc taaccagata
     7021 aaacgttgcc attgagatat agccatatag ggaataactg gctaaatcga taattgaaca
     7081 gcgatgttcc gtagagctgg tggttttctt tatgacaggt aagtgacgac ccggatgggg
     7141 tgggaacgta caacggtata aaaacagagc gttaataaac ataatgcctg cggcaatcat
     7201 aaaggaccaa ccgaaccaca gttcagccca cactaacagg ctgataatta gcggtaataa
     7261 aaattgtccg ctggaaacaa atgctttaat taaaatattg gctgtacccg gtgagcgtgg
     7321 aaaagcttcc atcaaactgg gataggtgcc tgcatcgaga aaactgtttg ccattcccgc
     7381 cagaaagcca aaaacataag cgataatgat gttattggtg tgcaggatgc caaaaaagaa
     7441 ggccatatag cagcacatcc cgagcatgat aaaagggcgg cgaccaaagc gatcggataa
     7501 taatcctgca aaaagcaaga cacttaatcg accaatgccc agtgatgaga taactatcga
     7561 gacgcccgcg gcattagtct gccaaagcgt ctccagcgag gccatattca ggctcatcaa
     7621 aatgacgccc ataccatgca ccaggtaatt aaaatacaac ccaagtgcgg tagggtaata
     7681 gggatttttc ataggttcta cttagcaaag gtgctttaaa aagtgtagcg atgttgtgtc
     7741 agtatccgtt cattattgtt gtaacaacga caattccttt ctgtggagaa attaacaaag
     7801 ccgctccaga tcgtgtgcat ataattcagc ggctaccgat tgatagatct tccagtcaat
     7861 aaaattgcca tcagggtaga cttgttgaaa cgcggttaag tcggggaaaa aacgcatggt
     7921 gttgttgccg atgcggttat ttattttctc tatgattgca tgaagatgtt tttttcgttg
     7981 ctgacgcata gccaacagtt gctcgactac ataatcagga atggcgcatt tgccattttc
     8041 ccactgacgc cagctttcac tgttaccggt ctgggcaatc caggtcgcgc attcatcaat
     8101 agtcatggca aaaatatggc gaagtgcctg gagttcataa gcgttcatcg tgtaatggct
     8161 gcatgaaata aacaataaaa tataaataac attatcagcc tgctgacggc ttgaagagag
     8221 tcacagtatc ttgtgcaaca ttattggaaa gagtgagggg cgaggtagcg aagcagaaaa
     8281 atgttaaaaa aaagcccatc gtgggagatg ggcaaagact acacacagca attcgttgtt
     8341 tcactcaggg gatttccatg cttataaatc aatatgttga tttataaccg tgagctaata
     8401 gtaggcatac ggactttttg cgtcgatcag attcgtctca ttagtttaaa aactgtaaag
     8461 aatttgcgac aggtactacc agctgaatag cggtaagcag gaaaatatgg ggaagagcaa
     8521 gcaaatatca attagcaggg gttaacaatc aggcggcaga gccgcctgat gagaaattac
     8581 ttattcgatt tctcgatttc gccaagttct tcgagaattt cttccggttg gtccgtcggc
     8641 ggtgggactt cttccaccca cgcggcaaac agacgccagg aaacggctaa cagaaccgga
     8701 ccaataaaca gaccgatcat cccgaaagca atcaaaccac caataacgcc agagaggatc
     8761 aggatcagcg gtaaatcggc acccatgcga attaacattg ggcggatgac gttatcgagc
     8821 gtgccaacca caccgctcca aactaacaat accgttcccc aggtggtatc gccagtccag
     8881 tagagccaga taatcgccgg aatcagtacc ggcaacggtc caagctggac aaggcaggag
     8941 aggatcatta acaccgttag caaagttgca taaggtacgc cggatacggc gaggccgata
     9001 ccgccaagca ctgcctgtac taacgccgtc accaccacac ccagcgccac cgcgcggata
     9061 gcctgtgccg ccagcagcac ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa
     9121 tggcgaatgc cttgtgccac ctgttcaccg cgccagtaca gcagggcgct gaagagaagc
     9181 atcagcgcac aatgcaccat aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg
     9241 gtggtggtgc caatataagg acggactttc gccatgatcg ccgtcccccc catatccagc
     9301 aaattgtgcc agcctgcata tagcttcgcg ccaatcaccg gaatggtatt aagccacgcc
     9361 agatcgggta acgtcatgtc accactggaa atggctttaa ttagcgggcc gctgccgtcg
     9421 acgatactgt taaccagcaa agcaataggg atgataaaca ccatcactaa taacagcgtc
     9481 atcaccagaa cggcgagaga gcggcgacca aacatgatct tttgcaaacg taacaatacc
     9541 ggccaggtgg cgataaccac tgtaccggcc catgcaaagc cgagaataaa gggttgtaca
     9601 atccacagac atgccacaat catgatggct aaaaacagca ccgaaagcag aatttgtgcg
     9661 acatccctgg gctgacgaac atttaccata actacttttc accttttttg tgcgtcaaat
     9721 cgtcgacgcg acgtgaacac gcgaaactca cctgcataat gatgagcaat ttcagcggtt
     9781 tttaacaggt cgattctgcc cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa
     9841 caactcaggc aacacgcaaa cgattaatct ctcatcacag accgcaggaa agggtcaata
     9901 taatgattcc acagatttcc caggcacccg gcgtcgttca actggtgctt aattttttgc
     9961 aagagctgga gcaacagggt tttaccggcg atacggcgac aagttatgcc gatcgtctga
    10021 caatgtcgac tgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt
    10081 caaccgcaga tgtagcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgttga
    10141 tctttacccc ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta
    10201 ttgttgatat gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg
    10261 tgcgcgttga ggccggggtg attaaagacc aactcaatca gtacctgaaa ccctttggct
    10321 acttttttgc gccagaactt tcgaccagca accgggcaac gctcggcggg atgatcaata
    10381 ccgatgcatc cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg
    10441 tacgcgcggt gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgaactgg
    10501 cggaaacgct gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac
    10561 gttgccgtca gcaacgccag ttaatcatcg acaacttccc caagcttaac cgctttctta
    10621 ccggttacga tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca
    10681 ttctgacggg ttcagaaggg acgctggcct ttattaccga agcgcgactg gatattacgc
    10741 gcttgcctaa agtacgccgt ctggtgaacg tcaaatatga ctcttttgac tccgcgctgc
    10801 gtaacgcgcc gtttatggtt gaggcgcgcg cgctttcggt agagacggtg gactcaaaag
    10861 tgctcaacct tgcacgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg
    10921 tgcctgataa agagatgctt gggctgaaca ttgtggaatt tgccggtgat gatgaggcac
    10981 tgattgacga gcgagttaat gcactctgtg tgcggcttga tgagctgatt gccagccaac
    11041 aggcaggagt gatcggctgg caggtgtgcc gcgagctggc gggtgttgaa cgtatctatg
    11101 cgatgcgcaa aaaagccgtt ggtctgcttg gcaatgccaa aggtgctgct aagccaattc
    11161 cgtttgctga ggatacctgc gtaccgccgg aacacctggc ggattatatt gctgaatttc
    11221 gcgcgctgct cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg
    11281 tcttgcacgt ccgtccggcg ctggatatgt gcgatccgca acaagagatt ttgatgaagc
    11341 aaatctctga tgatgtagtg gcgcttaccg cgaaatacgg tggtttgttg tggggcgaac
    11401 acggcaaagg ttttcgcgcc gaatacagcc cggcgttttt cggtgaggaa ctttttgcag
    11461 aactgcgtaa agtgaaagcg gcatttgacc cgcataaccg actcaacccc gggaagattt
    11521 gcccgccaga aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat
    11581 tcgatcgaca gatccccatt gcggtacgcc agcagtggcg tggtgcaatg gagtgtaacg
    11641 gcaacggttt atgcttcaac tttgatgctc gtagtccgat gtgtccgtcg atgaagatca
    11701 cccagaaccg gattcattca ccgaaagggc gcgctacgct ggtacgtgaa tggctgcgtt
    11761 tgttggcgga tcgcggcgtt gacccactca aactggaaca agaacttcct gaatccggag
    11821 tcagtttgcg gacgttaatt gcccgcacgc gcaatagttg gcatgcgaat aaaggcgaat
    11881 atgacttctc acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcgtgtt
    11941 cgactcagtg ccccatcaaa attgatgtgc cggagtttcg ctctcgtttc ctgcaactct
    12001 atcacacccg ttatttacgc ccgctgcgcg accacctcgt cgctacggtt gagagttacg
    12061 caccgctgat ggcacgcgcg ccgaaaacct ttaatttctt cattaaccag ccgctggtgc
    12121 gcaaactctc ggaaaaacat atcggcatgg ttgatttgcc gctgctgtcg gtcccctcgc
    12181 tacaacaaca aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc
    12241 tcaatgcaga gcagaaagcg cgcacggtgc ttgtggtgca ggaccccttt accagctatt
    12301 acgatgctca agtggtggcg gattttgtcc gtctggtcga aaaattaggt ttccagcccg
    12361 tgttactgcc attttcgcca aatggcaaag cccagcatat caaaggtttt cttaatcgtt
    12421 ttgcgaagac ggcgaaaaag acggcggatt tcctcaaccg tatggcgaag ctgggtatgc
    12481 caatggtagg cgtcgacccg gcgctggtac tttgttatcg cgatgaatat aaactggctc
    12541 tgggcgagga gcgtggcgag tttaacgtct tactggcgaa tgaatggctg gcaagcgcac
    12601 ttgagtcaca gccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta
    12661 ccgaagttac cgccttgccg ggcgcgccag cacaatgggc ggcaatattt gcccgttttg
    12721 gcgcgaaact ggaaaatgtc agcgtggggt gctgcggcat ggcagggact tacggacatg
    12781 aagcgaaaaa tcataaaaat tcgctcggga tctatgagtt atcctggcat caggcgatgc
    12841 agcgactgcc gcgtaatcgc tgtctggcga ccggatattc ctgccgtagt caggtaaaac
    12901 gggttgaagg cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat
    12961 atggaaacga aaaatcaccc tggaagcact gaatgctatg ggggaaggaa acatggtggg
    13021 gttgctggat attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt
    13081 agactcgcgg acaaagcagc ctttcgggtt gctgcatgga ggtgcatccg tggtactggc
    13141 cgaaagtatc ggttccgttg ccggttattt atgtaccgag ggtgagcaaa aagtggttgg
    13201 tctggaaatc aatgctaacc acgtccgctc agtacgagaa gggcgggtgc gcggcgtatg
    13261 taaaccgttg catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa
    13321 agggcgtttg tgctgttcgt cacgattgac gaccgctatt ttgtgacgtt gtcaccagaa
    13381 aagtgtgacg catattgctt ttggttatta tctgcacagt aaacggtggt atactgtgca
    13441 ggtcttgtct aaaagcgagg atgctatgtc tactcaactt gatcccaccc aactggccat
    13501 tgaattttta cgccgtgacc aaagcaatct ttcacctgcc caatatctca agcgtctgaa
    13561 acaattagaa cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga
    13621 aatctatttc gcctggcgtc tgggcgtgca ttgatcctga tttaatgtcg cgagttcgcc
    13681 aaatcggcct ggattaatct tcgtccaggt tcgagggcct tcttaatact gcctatctac
    13741 ccgcctgata acgttcaaaa ataatacaaa tcgcagtttg tgttaaaaca gtgggttagc
    13801 tttatgagat tatccttatc gccccttcaa gagctaagcc actgtgagtg ccggagataa
    13861 gcgccggatg gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt
    13921 tctgaacgct gcactttttt acctatccct taaatagtca ttttcaatgc aactttttct
    13981 ttttcatcac agccttaatg ctccttagat aataatcatt atctaacaat gagataccta
    14041 attcttagcg tgcctgttaa cccacaaatc agggtctatg cttattaaaa gcaggtaaaa
    14101 agcatgtttt acgctaaagc ccctgcgttt gctgggttga actgataatc attatcacta
    14161 acatgctgtt atacgctgaa agcgatgaag tgaggtaaat cgatggacat gcattcagga
    14221 acctttaatc cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata
    14281 cacatccgcg aactggtggc aaagcagccg ggtatgatcg gcgtgcgctt aggcgtgaag
    14341 caaacgggct gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac
    14401 gatctgctgt ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaagc gatgcctttt
    14461 attgatggca cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac
    14521 aaccctaaag cccagaatga atgtggctgt ggcgaaagct ttggggtata ggcggtacta
    14581 tgtctcgtaa tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaatt
    14641 ataaagaagg attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag
    14701 aggtggtgcg cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc
    14761 tcaacgccta tcacgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg
    14821 acaagctgaa ttatcaggat tacagctact actcggcacc atcgtgcggt aattgtgacg
    14881 acaactgcgc gtctgaaccc ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta
    14941 aagaggtgga ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg
    15001 cggtggatgc cattttcgac tctgtttcgg ttgccactac ttatcgcgaa aaactggcgg
    15061 agcagggaat tattttttgt tcctttggcg aggcgatcca cgatcacccg gaactggtgc
    15121 gtaaatatct cggcaccgtg gtgccgggga atgacaactt ctttgccgca cttaatgcgg
    15181 cggtagcctc tgacggtacg tttatttatg tgcctaaagg cgtgcgttgc ccgatggaac
    15241 tttccaccta ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc accattctgg
    15301 tggccgacga agacagctac gttagctaca ttgaaggctg ttccgctccg gtgcgtgaca
    15361 gctatcagtt acacgcggcg gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat
    15421 attctacggt acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg
    15481 tcaccaagcg tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa
    15541 ccgggtcagc gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg
    15601 gtgagtttta ctcagtggcg ctgaccagcg gtcatcaaca agcggatacc ggcaccaaga
    15661 tgatccacat cggtaaaaac accaaatcga ccgttatctc gaaagggatc tctgccggac
    15721 atagtcagaa cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca
    15781 atttcactca gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt
    15841 atgtcgaatg tcgcaataac agcgctcagc tggagcacga ggcaacgaca tcacgtattg
    15901 gtgaagatca actgttttac tgcctgcaac gcgggatcag cgaagaagat gccatctcga
    15961 tgattgttaa cggtttttgc aaagacgtgt tctcggaact gccgctggaa tttgccgttg
    16021 aagcacaaaa actcctcgcc atcagccttg agcatagcgt cggataagga ataaacatgt
    16081 taagtattaa agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc
    16141 tcgatgttcg tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta
    16201 ccttatcggc aacgcttgcc gggcgagaag attatgaagt gacgagcggc acggttgagt
    16261 ttaaaggcaa agatttgctt gcgctgtcgc cggaagatcg tgcaggtgaa ggcatcttta
    16321 tggccttcca gtatccggtg gaaattcccg gcgtcagtaa ccagtttttc ctgcaaacgg
    16381 cacttaatgc ggtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg
    16441 atttgatgga agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg
    16501 taaacgttgg tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc
    16561 tggaaccgga gttatgcatt cttgatgagt cagactccgg gctggatatt gacgcattaa
    16621 aagtggttgc cgatggcgtg aactcgctgc gtgacggcaa gcgttcattc atcattgtta
    16681 cgcactatca acgcattctc gactacatca agcctgatta cgtccatgtg ctgtatcagg
    16741 gacgaattgt gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg
    16801 gctggcttac cgaacagcag taacgcactg caacagtggc atcatttgtt tgaagctgaa
    16861 ggggcgaaac gctccccgca agcacagcag catttacaac aattgctgcg taccggactg
    16921 ccgacacgta aacatgaaaa ctggaaatat acgccgctgg acgggctgac caatagccag
    16981 tttgtctgca ttgcggggga gatatcccca cagcagcgtg atgccttagc gttaacgtta
    17041 gacgccgtgc ggctggtgtt tgtcgatgga cgttacgtgc ccgcactgag cgatgcaact
    17101 gaaggcagcg gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt
    17161 caggcggaag tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc
    17221 gtgaagcgcg gtcaacggcc gacaaagcca ttgctgttaa tgcatatcac ccagggcgtg
    17281 gcaggtgaag aggtgaatac tgcccattac cggcatcatc tggatctggc ggaaggtgcc
    17341 gaagcaacgg tgatcgaaca ttttgtcagc cttaatgatg ctcgtcactt caccggcgca
    17401 cggttcacta tcaacgtcgc agcgaacgcc catttgcagc atatcaagct ggcgtttgaa
    17461 aacccgctca gtcaccactt tgctcataac gatttgttgc tggctgatga tgccaccgca
    17521 tttagccaca gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc
    17581 aatggcgaaa acagcacggt gcggatcaat agcctggcga tgccggtgaa aaacgaggtg
    17641 tgtgatactc gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac
    17701 aaaactatcg tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag
    17761 cacgccatca aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg
    17821 gaagtggata ctaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc
    17881 gcgacggtgg ggcgtattga tgacgaacag atgttttatc tgcgctcgcg cgggatcaat
    17941 cagcaagatg cccagcagat gatcatctac gcctttgctg ccgaactgac ggaagcactg
    18001 tgtgatgagg ggcttaaaca gcaggtgatg gcccgaatcg gtcaacgact gccaggaggt
    18061 gcaagatgac tttttccgtc gacaaagtgc gggccgactt tccggtgctt tctcgtgagg
    18121 taaacggttt gccgctggct tatctcgaca gtgccgccag tgcgcagaaa ccaagccagg
    18181 tgattgacgc cgaggctgag ttttatcgtc acggctacgc ggcggtgcat cgcggtattc
    18241 ataccttaag cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt
    18301 ttattaatgc ccgttcggcg gaagagctgg tgttcgtccg cggcacgacg gaagggatca
    18361 atctggtcgc caatagctgg ggcaacagca atgtgcgagc gggcgataac atcatcatca
    18421 gtcagatgga gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg
    18481 cagagctgcg tgtgatcccg ctcaaccccg acggtactct gcaactggag acgctgccta
    18541 cgctgtttga tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggga
    18601 cagaaaatcc gctggcggag atgatcacac ttgcgcatca acatggcgca aaagtgctgg
    18661 tggatggcgc tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgatt
    18721 tctacgtgtt ttccgggcat aaactgtatg gtcccaccgg cattggcgtg ctgtatgtca
    18781 aagaagcctt gttgcaggag atgccgccgt gggaaggggg cggttctatg attgccaccg
    18841 ttagcctgag tgaaggcact acctggacca aagcgccgtg gcggtttgaa gccggtacgc
    18901 ccaataccgg aggtattatt ggccttggcg cggcgctgga gtatgtttcg gcgctggggc
    18961 ttaataacat agccgagtat gaacagaatc tgatgcacta cgcgctagca cagctggaat
    19021 ctgtaccgga tctcactctc tatggcccac agaacaggct tggcgttatt gcttttaatc
    19081 tcggtaaaca ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc
    19141 gtaccggaca tcactgcgct atgccattaa tggcctatta caacgtcccc gcgatgtgtc
    19201 gggcgtcgct ggcaatgtac aacacccatg aagaagtgga tcgtctggta actggattgc
    19261 aacgtattca tcgtctactg gggtaaaagg gaggctctat ggctgtattg ccggataaag
    19321 aaaaattgct gcgtaacttt ttacgctgcg ccaactggga agagaaatat ctctacatta
    19381 ttgaactggg acagcgtctg ccagaattac gtgacgaaga caaaagcccg caaaatagca
    19441 tccagggctg ccagagtcag gtgtggattg taatgcgcca gaatgcgcag ggaattattg
    19501 aattgcaggg cgacagcgat gcggccattg taaaagggct tattgcggtc gtctttattc
    19561 tttacgatca aatgacgccg caggatattg tcaatttcga tgtgcgcccg tggtttgaaa
    19621 aaatggcgct cacccaacat ctcaccccat cacgctcaca aggtctggaa gcgatgattc
    19681 gcgcaattcg cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt
    19741 tggctcacat tgtcgggtgg tcaatgttta cactggcctg cacaggagtg ttttggtttc
    19801 aggtgaacta agcgccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta
    19861 caatgaaacg cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg
    19921 ctgcctgggc ggttgattat ccgctaccac caaccggcag ccgactggtt gggcaaaatc
    19981 aaacgtatac ggtgcaagaa ggggataaaa accttcaggc catcgcccga cgttttgata
    20041 ctgcggcaat gttgatcctt gaagccaata acactatcgc cccggtgcca aaacccggta
    20101 cgacgataac tattccttca caactgttat tacctgatgc accgcgccag gggataattg
    20161 tgaaccttgc agaactgcgc ctttattatt atccgccggg agaaaatatc gtgcaggttt
    20221 atccgatagg tattggtttg caggggctgg aaacgccggt gatggaaacg cgtgtggggc
    20281 agaaaatccc taacccaacc tggacgccta cggcgggcat tcgtcaacgt tcgctggagc
    20341 gtggcattaa attaccgccg gtcgttcctg ccgggccaaa taacccgcta ggacgttacg
    20401 cactgcgcct cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca
    20461 gcgtcggttt gcgcgtcagt tcgggctgta ttcgcatgaa tgcaccggat attaaagctt
    20521 tattctccag cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt
    20581 ccgtggaacc taacgggatg cgttatgttg aagtacatcg accactatcg gcagaagaac
    20641 agcagaacgt tcagacaatg ccgtacacgc tgccagcagg ctttacgcaa tttaaagaca
    20701 ataaggctgt agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg
    20761 ttgcggtgag cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc
    20821 agaatgggga accagagcaa gggaatatgt tacgcgcgac gcagtagcgg taaatagcag
    20881 gcaaaaaaat ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt
    20941 atttagtagc catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct
    21001 gaacgtcgga acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag
    21061 tctgaacgtc agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag
    21121 tagaacccag gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct
    21181 agattgagtt aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa
    21241 tatttttttg atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct
    21301 gggttttaga taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttagata
    21361 aagtgagatt atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa
    21421 taaaaaaagc gcccatcagg acgcttcgat atacaaatta attcacaaaa gcaatattac
    21481 aggacgtgaa cagatgcggt gttagtagtg ccgctcggta ccagtgcacc agaaaccata
    21541 actacgacat cacctttgtg tgccagaccg ctctgcagag ccagttcttt acccagacgg
    21601 tagaagtcat cagtagaagt gatctcttta accagctgcg gcacaacgcc tttgctcagt
    21661 actaactggt gagccgtttt ttcgttggtg gtcagtgcca ggatggtggc atccgggaag
    21721 tatttacgta ctgcgcgagc agatttaccg ccctgagtag caaccacgat cagcggagca
    21781 tccagttttt cagcagtttc aacggcacca cggcataccg cttcggttat acgcagttta
    21841 cggttgtcat tgttgaactc gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg
    21901 gtcgccatga tagaaaccgc ttccagcggg tatttacctt ttgcggattc accagacagc
    21961 atcactgcgt cagtaccgtc gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc
    22021 gggcgtgggt ttttgatcat ggaatccagc atctgggtcg cagtgataac gactttacgt
    22081 gcacggatac atttttcgat catcatcttc tgggcgaaga taacttcttc taccgggatt
    22141 tctacaccca ggtcgccacg cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg
    22201 aagttgttga ggccttcctg gttttcgatt ttggagatga tgtggatgtt ttcgccgccg
    22261 tgcgctttca ggtgctcacg gatttcgata acgtcagaac gcttacgaat aaaggaagca
    22321 gcaacaaagt ctacgccttg ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc
    22381 agtgctggca gggcaatgga aacgccaggc aggttcacac ctttgttttc gcccaggtcg
    22441 ccgttgttca gcactttaca gataactttg ttaccttcaa tggcggtaac ttccataccg
    22501 atcagaccgt cgtcaaccag tacggtgttg ccaacagaca ggtcagtagt gaaaccttca
    22561 tacgttaccg caaccatttc gctgttgccg ataacagatt tatcagtagt gaaagtaaag
    22621 gtctgacccg ctttcagaga aacgtcgtta ccgccttcca gtttcatggt gcggatttcc
    22681 ggacctttgg tatcaagcag gatagcggcg gttttaccag ttttgctcat cacgttgcgc
    22741 agattctgaa tgcgctgacc gtgttctgca tagtcaccat gagagaagtt cagacgcata
    22801 acgttcatgc cagcgtccag cattttagct aacatctctt cagattcggt tttcggtccg
    22861 atggtgcaaa caattttggt ctttttcatg acagtcttag tctttaagtt gagaaggatg
    22921 ggagaaactt gctttctggg cgcactgccg gaaagcgaat ccggtattac gaaagtggtt
    22981 gtgcctcgct ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt
    23041 ctcagcccaa cgtgagtgcc tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa
    23101 accattcaag agtcaattgg cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa
    23161 aacagcgttt caaaaaatat acaacttgtt agacaataaa ttgttatcaa agattcagcg
    23221 ttccagggaa aaggttacgc aaaaacttcg ttgtttgcgc aacgtctggt gccggggttg
    23281 tcagtgtgag gataagtgaa agttcatccg gtgtataaat acatcatcgc tcaattgcta
    23341 cagttaaaat aatttatcac gttatttgtc tgatttt
//
LOCUS       DACADF010000074        23071 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.074, whole genome shotgun sequence.
ACCESSION   DACADF010000074 DACADF010000000
VERSION     DACADF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.074"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..676)
                     /locus_tag="ISB30_002712"
     CDS             complement(<1..676)
                     /locus_tag="ISB30_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990327.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSW
                     NPAYNCFDRPGKKCISFTLHINGKDHDLLAVDKIHVDLNWRYLNEYLDHISANDEVLF
                     LKQGHEI"
     gene            complement(853..1764)
                     /gene="gsiD"
                     /locus_tag="ISB30_002713"
     CDS             complement(853..1764)
                     /gene="gsiD"
                     /locus_tag="ISB30_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAP1990328.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1767..2687)
                     /gene="gsiC"
                     /locus_tag="ISB30_002714"
     CDS             complement(1767..2687)
                     /gene="gsiC"
                     /locus_tag="ISB30_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAP1990329.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGILLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2705..4243)
                     /gene="gsiB"
                     /locus_tag="ISB30_002715"
     CDS             complement(2705..4243)
                     /gene="gsiB"
                     /locus_tag="ISB30_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAP1990330.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKEVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4263..6134)
                     /gene="gsiA"
                     /locus_tag="ISB30_002716"
     CDS             complement(4263..6134)
                     /gene="gsiA"
                     /locus_tag="ISB30_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAP1990331.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQESSIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6121..7086)
                     /gene="iaaA"
                     /locus_tag="ISB30_002717"
     CDS             complement(6121..7086)
                     /gene="iaaA"
                     /locus_tag="ISB30_002717"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP1990332.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACLMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7290..8525
                     /gene="moeA"
                     /locus_tag="ISB30_002718"
     CDS             7290..8525
                     /gene="moeA"
                     /locus_tag="ISB30_002718"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAP1990333.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEARSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASPLKKSPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            8525..9274
                     /gene="moeB"
                     /locus_tag="ISB30_002719"
     CDS             8525..9274
                     /gene="moeB"
                     /locus_tag="ISB30_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAP1990334.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9350..10012)
                     /gene="fsa"
                     /locus_tag="ISB30_002720"
     CDS             complement(9350..10012)
                     /gene="fsa"
                     /locus_tag="ISB30_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP1990335.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAVGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            10143..11042
                     /locus_tag="ISB30_002721"
     CDS             10143..11042
                     /locus_tag="ISB30_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAP1990336.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLSYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11048..13480
                     /locus_tag="ISB30_002722"
     CDS             11048..13480
                     /locus_tag="ISB30_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAP1990337.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13626..14441
                     /gene="ybiV"
                     /locus_tag="ISB30_002723"
     CDS             13626..14441
                     /gene="ybiV"
                     /locus_tag="ISB30_002723"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAP1990338.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHITLDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMSNAAENIKQIARYATDDNNHEGALNVIQAVLENKPPFNL"
     gene            complement(14641..16233)
                     /locus_tag="ISB30_002724"
     CDS             complement(14641..16233)
                     /locus_tag="ISB30_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAP1990339.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            16452..17372
                     /gene="ldtB"
                     /locus_tag="ISB30_002725"
     CDS             16452..17372
                     /gene="ldtB"
                     /locus_tag="ISB30_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAP1990340.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(17430..18548)
                     /locus_tag="ISB30_002726"
     CDS             complement(17430..18548)
                     /locus_tag="ISB30_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAP1990341.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGGQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPVLQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(18545..19012)
                     /locus_tag="ISB30_002727"
     CDS             complement(18545..19012)
                     /locus_tag="ISB30_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAP1990342.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            19198..19326
                     /gene="mntS"
                     /locus_tag="ISB30_002728"
     CDS             19198..19326
                     /gene="mntS"
                     /locus_tag="ISB30_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAP1990343.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            19598..21181
                     /locus_tag="ISB30_002729"
     CDS             19598..21181
                     /locus_tag="ISB30_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAP1990344.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(21230..21745)
                     /gene="ompX"
                     /locus_tag="ISB30_002730"
     CDS             complement(21230..21745)
                     /gene="ompX"
                     /locus_tag="ISB30_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAP1990345.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(21798..21863)
                     /locus_tag="ISB30_002731"
     CDS             complement(21798..21863)
                     /locus_tag="ISB30_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990346.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            22098..22985
                     /gene="rhtA"
                     /locus_tag="ISB30_002732"
     CDS             22098..22985
                     /gene="rhtA"
                     /locus_tag="ISB30_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAP1990347.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         5699 a         6271 c         5629 g         5472 t
ORIGIN      
        1 tgatctcatg accttgcttt aaaaatagaa cttcatcatt agcgctgatg tgatcaagat
       61 actcgttcag atatcgccag tttaaatcga catgaatttt atctaccgct aacaaatcat
      121 gatctttgcc gttgatgtgt agcgtaaaag agatacattt tttacccgga cgatcaaagc
      181 aattgtatgc cgggttccag ctgttgttag agtcatctga agccatcgac cacggtcgtt
      241 tcgatgggtt aaaatcggca aaaataaccc gccctgactc gtcgacaagc ggtccattta
      301 cttgttcctg atgataaaca acgattttat cattcgccct ccgcgaaaag agataatatt
      361 tattatcatc aagtaccagt cccaccgagt ctaaaaatgg catcatttcc attttcagaa
      421 tcacggcacg ttgaatctcc tctgtatttt ttaataaata cagtgagtcc tttaatgtca
      481 gggccgaacg ttcaatatct ttaaagaaat agccaacgtt tttctgaata agttcaaagc
      541 ggctatcggc acgttttacc gcgcgttccg ataccagttg atagttaatg atgaaaaaaa
      601 gaccaataaa tgacaatgct gccaataaaa ataaaattat cagccttatc tttatctttt
      661 cgtataaact cagcatgtta atacatttaa aaaaataact gtctgaacgc tgtgccgctg
      721 caaaatagtc ccttgctgcg gctttttaac caaattaccc gattctacaa aatcagcaga
      781 aagttacaac acttgcttag ggcaataatt tcctgtgagg tgattaccct ttcaagcaat
      841 attcaaacgt aactatcctt taattttcgg atccagcgca tcgcgtaaac catcgcccaa
      901 caaattgaac gccagtacgg tcagaaaaat agccagggcc ggaaatacag cgacatgcgg
      961 cgcgataacc atatccgctc gtgcctcatt gagcattgca ccccactctg gtgtcggcgg
     1021 ctgcgcaccg aggccgagaa atgacaggct ggcggcagag ataatcgagg tgccaatgcg
     1081 catggtgaaa aacaccacga tagaagagac ggtcccaggc aagatatgac gcaacaaaat
     1141 ggtcatatcg ctggcaccaa tactgcgtgc tgactcgata aaggtttgct gtttcaacac
     1201 cagcgtgttg ccgcgcacca gccgggcaaa cgcggggatg gaaaaaatgg cgactgcgat
     1261 aatcacgtta gcaatgccgc ttcccaacac cgcaacaaca gcgatcgcca gtaaaatacc
     1321 cgggaaggca aacagcacat cgcaaatgcg catgatcagc cgatcccacc agccttcata
     1381 atatccagcc agcaagccca gcaacgtccc gatcgccgca ccgataaaca cggcaaacac
     1441 gcccgccgcc agcgagattt gcgcaccaac caggacacgg ctgaaaatgt cgcgccccag
     1501 tgaatcgaca ccaaaccagt gctgcaaaga aggtccgtta ttcagattgt cataatcaaa
     1561 ataattttcg gcgtcatagg gagcgatcca gcgtgcaaaa atggccacca caatcaataa
     1621 aataacgaac aatgcggcgg tcatcgccat atgctgacgg cgaaatcgtc gccagaattc
     1681 atgccacggt gtacgtacct ggtcaggttt gaccagtggc atggcgttta acaccgcctg
     1741 acgtcgccag ttaaatagtc gcatccttac ttgtacctga tagccgggtt aatggcggcg
     1801 taaagcacat ccaccactaa gttgataaga ataaattcca gcgagaaaag cagaatttcc
     1861 gcctgaatca ctgggtaatc gcgcatttct acggagtcaa cgagtaagcg tccaagtccg
     1921 ggccagttga aaactttctc caccacgatg gaaccaccga gcaagaagcc aaactgtaag
     1981 cccatcatgg tcactaccgg gatcatcgcg ttgcgtagcc cgtgtttgag gacaacccag
     2041 gtttcgctca cccctttcgc cctcgcggta cgcatataat cttcgcttaa aacgtcgaca
     2101 aacgacgcgc gggtaaagcg cgccatcacg gcggccactg ccgcgccgag cgtcagggag
     2161 ggtaaaatgt agtgctgcca actgtctgct cccacggtag gcagccagcc cagttcaacg
     2221 gagaatacct gaattaaaag tatccccaga gcaaatgccg gaaacgagat ccccgacacc
     2281 gcaatggtca tactcaatcg atccggccaa cggttacgcc agacggcggc gataattccc
     2341 gccgccatac caaatatcac cgcccagacc atactggtta tggtcagcca gagcgttggc
     2401 ataaagcggc tggcaatctc atcggcaacc ggacgacgcg acaccatcga caggccaaaa
     2461 tccccctgca cagcattgct gatatagtgc cagaactggt gatacagcgg ctgatccaac
     2521 cccaactgct gacgcaccag ttctataacc tgcgcatcag cttcgggccc ggcaatcaat
     2581 cgcgccggat cgccgggcag catatggaca aataaaaaca ccagcaccga gacgataaac
     2641 agcgtcggaa tcaaccccag taagcgtttg ataacgtaat taagcattcc actccctgcg
     2701 ttgcttattg caaatccgcg tcttcaaagc tgaagccggt gtctggcatg atccaaaaac
     2761 cggtcaggtt tttactgtgt gccgacacca gtttttctac caccagcggg atccacggcg
     2821 actcttgcca gatgatatcc tgcgccgcct tatacaagcg ggtcttttcc gccggatcat
     2881 tagttttcag tgcctgagcc aggaagtcat ccacctgttt attgctgtaa aacgcggtat
     2941 taaacagcgt tggtggccag ttctgcgagg caaacagcgg cgatagagcc cagtcggctt
     3001 cgccggttga agccgaccag ccagtgtaga acatccgcac gccgctctct ttttgccctt
     3061 taccttcaac ttctgccgcc cgctgtccgg catccatcgc agtcacctgg gctttaatcc
     3121 caacctgcgc taactgctgc tgggtaaatt gcagcacttt ctgcgcggtg ctgtggttat
     3181 gtgacgacca cagcgtggta ctgaaaccgt tgggatatcc cgcctctttc agtaattcgc
     3241 gcgctttcac tggatcgtaa ggccacggtt tataactttg cgcgtaggcg atactcggcg
     3301 gtaccacacc agtagctggc gttgcatagc ccgcgaaggc cactttcacc agcgccgggc
     3361 ggttaatggc gtaattcagc gcctcacgga ccttcgggtt atcgaacggc ttttgcgtca
     3421 cgttcatact gatataacgc tgcataattg acggactggc catcaactcg atatttttgt
     3481 ttttctccag cagtgcggcc tgctcgtagg gaatggggaa agcaaactgc gcttcaccgg
     3541 tttgcagcat tgccgcgcgg gtgttgttat ccgccaccgg acgccaggtt atgctgtcca
     3601 gtttgggcaa tcctggctgc cagtaacccg cgaatttttt caccttcaca aaatcggtct
     3661 gattccaggt atccagttca tacggcccgg ttcccaccgg atgaaaacca atctccttgc
     3721 catatttttc cagcgctgcc ggtgatatca tcgcggtcgc cggatgggca agaatattaa
     3781 taaacgctga gaatggctgt ttgagggtaa tctttaccgt tgtcggatcg atcgcttccg
     3841 ttttggcgat attcttatac aggttatagc gtttaagatg attcgccgga tcgctggccc
     3901 ggtccagatt cgctttcacc gccgctgcgt tgaaatcggt gccatcctgg aatttaatgc
     3961 cttcccgcaa tttcacggtg taagtaaggc cgtcatcgga aacggtgtaa ctctccgcca
     4021 gcacgttttt cagtttcatc tctttatcca gaccgaacag cccctggtaa aacgatttcg
     4081 ctacggcctg agataacgtg tcatttgcgt catacggatc gagcgtggtg aagttcgatc
     4141 ccaccgccac caccacctct ttggcagcga atgcacaaga tgccatcaac gctgtcgcaa
     4201 tgcccagcgc cactaaccca ctacggtgta cagctcttgc cattttattc tccgcctgaa
     4261 tgttatctac gcatgaatgc gtattctgat tgtggttgtg cgacgtaatg ccccggcccg
     4321 acgcattgca acgagacggc tgcaacctct tcgccacgca gatgaatatt gctgggaaga
     4381 tcgtccgaca gcagtacacg ctgcggtcgt tgtcgggacg gttcagcgac cggaactgcc
     4441 gccagtaatt tacgcgtata aggatgctgc ggattttcaa agaccgcgcg ccgtggacca
     4501 atttcaacaa tttgcccgag atacatcacc gccacacgat gactaatccg ctcaaccacg
     4561 gccatatcgt gggagataaa cagatacgca atgccgaaat cacgctggag atcgagcaac
     4621 aagttgataa tctgcccgcg aatagaaaca tccagcgccg agacggcttc gtcggcaatg
     4681 atcacttttg gattcaatgc caacgcgcga gcaatgcaga tgcgctggcg ctgaccgccg
     4741 gaaaactcat gtgggtaacg ccaggcatgt tccggtaaca ggcccacgcg ctccagcaac
     4801 cacgcaacgc gtgcagccgc ttctttacct ggcaataaac cgtgtacgcg cagcggttcg
     4861 agaatcgaat caccgatagt ctgacgtggg tccagcgaag cgtaagggtc ctgaaaaata
     4921 aactgaatat cgcggcgcaa tgcctgaagt ttaccgggtg acaaggtatc gattcgctga
     4981 ccgttaaaga taatttcgcc gccctgcgat tcgaccaggc gcaacaacgc ccgcccggta
     5041 gtggatttac cgctgccaga ctcgcccacc agcgatagcg tttcgccagg ccagagatca
     5101 aaactgactt tctcaacggc atgcacttcc cgcgttacgc gattcaacaa accgctgcgc
     5161 aaagggaaac gggtgaccag attacgcacc cgtaaaacag gttcgccatc caccaccgtt
     5221 ttctgctcga tggatgactc ctgtttcgct ggatgttcaa gcgatatcaa tgggaaacgt
     5281 cggggataat ctaacccttt catcgcacca agttgcggaa cagcagctaa cagcgcacgg
     5341 gtataaggat gttgcggtgc atgaaaaatc tgttcgacgc tacccgtttc caccgcctcg
     5401 ccctgataca tcaccagtac ccgatcggca atctctgcca ccacacccat atcgtgagtg
     5461 ataaagataa cgcccatcga catctctttt tgcaatactt tgattaattg caggatctgc
     5521 gcctgaatag tgacatccag cgcggtggtt ggctcatcgg caatcagcac cgccgggcgg
     5581 catgacagcg ccatcgcaat catcactcgc tggcgcatcc cgccagagag ttgatgcgga
     5641 taacgtgaaa gaatggtttg tgcctccgga atgcgtacct gatccagcat ccgcttcgcc
     5701 tcgaccatcg cttcttcacg actggcgttc tgatgcagac gaatagattc agcaatctgt
     5761 tcacccacag taaataccgg gttcagcgat gtcatcggct cctgaaatat catcgccata
     5821 tccgcaccgc gcacatggcg catttgtgca gcgctctgct cgctaagttc aattacttcg
     5881 cgactgcgcc gctgcaacag cattttatcg cactgcacta aaccgcccgc ctgttccaac
     5941 aggcgcatca atgccagcgc agtcactgac ttaccggagc cggattcgcc aacaattgcc
     6001 agcgtctcac cacgttgcag gctaaaagag agattgcgga ccgcagctat tttctgctgc
     6061 tcctgcataa aggcaatatt cagattttca accgccagca catcaccggc atcaagttca
     6121 tcactgtgtg gcaacggtgt cccctttttc acggtagata ccggtggttg gcgtatcgcc
     6181 tgcgtagccc caggcgcgat acattccttc ggtgttaaac ggtagcgcga cattcccttc
     6241 atggtcgata gcgattaagc caccgctacc gccaagcgca gggagttttt ccattactac
     6301 ccgctcgcag gcttccgcga gacttaatcc gccgtaatcc attaacgcgg cgatatcata
     6361 tgccgccagc gcgcggatga agacttcgcc cgtaccggta caggaaaccg ccacactggc
     6421 gttattggca tagcatccgg cccccactaa gggactatcg ccaactcgtc cgggtaattt
     6481 attggtcatt ccgcccgtgg acgtggctgc cgccagattg ccgtctaaat ccaacgccac
     6541 ggcccctacg gtgcccattt tctgtttttc atccagtggc gcaccgctat ggtcgaggac
     6601 tgttgcccct tcctcgcgcg ctgccattag ttgttcataa cgcaaaggtg tggagaaaat
     6661 ctccggcgag acgcgctcca tgccatgagc aaacgcaaaa ttttctgccc cttcgccaat
     6721 catcatcaca tgcgggcttt gctccatcac cagccgggcg gcaagaaccg gattacgcag
     6781 atgactaacg cccgccaccg caccggcttt cagggtgtta ccatccatca gacaggcgtc
     6841 cagttcatgg gtttcatcac gcgtaaagac agcgccaatt ccggcgttaa acagtggaca
     6901 ctcttccagc agacgcaccg cttccgtcac cacatccagc gcactttcgc ccgctaccag
     6961 cattttctgc ccggtttcaa caatggcaga caacgcctcg atgtagcgta attcctgttg
     7021 cagactcatc tgcgcgcggc taattgcacc tgcgccacca tgaattgcaa tgactgcttt
     7081 gcccatagta ttcgtccatt aagagtggct cgccactttt gctataaata tcagaatttt
     7141 tatgaatgac tcattcaatt tttgaatata gaaagatgga tatggcatgt aaaggcagga
     7201 gccagcgcta cgcccgaggt ggcatacagt ctgtggcgtt tttctgacat aataggcaaa
     7261 ttcgattttg cctccgcagg agtgttttca tggaatttac caccggattg atgtcgctcg
     7321 acaccgcgct taatgagatg ctttctcgcg ttaccccact gaccgcccag gaaacgctgc
     7381 cactggttca gtgttttggc cgtattctgg cgagcgatgt cgtttcgcca ctggatgtcc
     7441 cggggtttga taactccgct atggacggct acgcggtgcg tttagccgat attgcctccg
     7501 ggcaaccgct gcccgttgct ggtaaatcct ttgccggtca gccgtaccat ggtgaatggc
     7561 ctgcgggtac ctgcattcgt attatgaccg gtgcgccggt gccggaaggc tgcgaagcgg
     7621 tggtgatgca ggagcagact gaacaaacgg acaatggcgt gcgttttact gctgaagcgc
     7681 gtagcgggca aaatattcgc cgtcgcggtg aagatatctc tgcaggtgcg gttgttttcc
     7741 cggcggggac tcgcctgact accgctgaac tgccagtgat tgcttcgcta gggattgccg
     7801 aagttccggt gattcgtaaa gtgcgtgtag cgcttttttc taccggtgat gaactccagt
     7861 tgcccggtca gccgctgggc gacggccaaa tctacgatac caaccgtctc gccgtacacc
     7921 tgatgttaga acagttggga tgcgaggtaa tcaacttagg gattatccgc gacgatcccc
     7981 atgccctgcg cgccgcattt attgaagccg acagccaggc ggatgtggtg atcagttccg
     8041 gcggtgtttc agtgggtgaa gcggattaca ccaaaacgat tcttgaagag ctgggggaga
     8101 tcgccttctg gaaactggcg attaaaccgg gtaaaccgtt cgcgttcggt aaactttcta
     8161 atagttggtt ctgcggcctg ccgggcaacc cggtttcagc gacgctgacc ttctatcaac
     8221 tggtacagcc tttgctggca aaactaagcg gcaataccgc cagcggcctg cccgcgcgcc
     8281 agcgtgtacg cacggcatcg cctctgaaga aatcgccagg acgccttgat ttccagcgcg
     8341 gcgtgctgca acgcaacgcc gatggcgaac tggaagtgac gaccaccgga catcagggtt
     8401 cacatatatt tagctccttt agcctcggca actgctttat cgtgctggaa cgcgatcgcg
     8461 gcaatgtgga agtgggcgaa tgggtggaag tagaaccgtt taacgcgttg ttcggaggcc
     8521 tgtaatggcg gaactcagcg atcaggagat gctgcgctac aaccggcaaa tcattctgcg
     8581 cggttttgat tttgacggtc aggaagcatt gaaagattct cgcgtgctgg tcgtcggcct
     8641 tggtggactc ggctgcgcag cttcgcagta tctggcaagc gccggtgtcg gcaacctgac
     8701 gctgctcgac ttcgacacgg tttcgctctc caatctgcaa cgccagacgc tgcacagtga
     8761 tgccacggtc gggcaaccaa aggtggaatc cgcccgtgac gccctgacgc ggatcaaccc
     8821 acatatcgcg attacgccag tcaatgcgct gcttgatgac gctgaacttg ctgcgatgat
     8881 tgcaaaacac gatctggtgc tcgactgtac ggataacgtt gcagtacgta atcaactgaa
     8941 cgcaggctgt tttgccgcga aggtgccgct ggtttccggc gcggcaattc gtatggaagg
     9001 gcaaatcacg gtcttcactt atcaggacgg cgaaccgtgc tatcgctgcc ttagccgttt
     9061 gtttggcgag aacgcgttaa cctgcgtaga agcaggcgtg atggcaccgt taatcggcgt
     9121 aattggttcg ttgcaggcaa tggaagcgat caaactgctg gcaggttatg gcaaaccagc
     9181 cagcgggaaa atcgtgatgt acgatgcgat gacctgtcag tttcgcgaaa tgaaactgat
     9241 gcgcaatccg gggtgtgagg tgtgcgggca gtaactgcca cctccaggcc agataagacg
     9301 cggcactccg ccacgtcatc agcaatttat gaggatgcag aacgtccggt taaatcgacg
     9361 ttctgccaaa cgctccctgc cagtcctgct caaacttcgc cacagcggca tcaaccgccg
     9421 gatagctaat catctgttgt gccacatcca gtggcagagt aattgattca caccctgcca
     9481 gtaagcagtc cagcgcctga cgcggggttt tgaaactcgc ggctaacact ttcgcctgcg
     9541 gcgcatgcat tttcaataac tggtgtaagt cggtcacagt ctgaataccg ctaccgccct
     9601 gagcatcaat acgattaacg taaggcgcaa catattccgc acctgccagc gccgacagta
     9661 gcccttgtgc tgcgccatat accgcggttc ccagcgtcgg aatcccttcc gcttttaaca
     9721 tcttaatagc tgccagcccc tctgcggtca ccggaacttt caccacgata tccgcaataa
     9781 tagaacgcaa tttacgtgcg tcattcacca tcccttcagc agtggtagcc attacctggg
     9841 caaacagacg cccctgaccg cccatcgcct cgtgaagttg cggaagcaca acctccagcg
     9901 gttttttacc cactgcgata atgcttgggt tagtggttac acccgccagc ggaaaaatac
     9961 gtgacagcgc cttcaccgca acaacgtctg aagtatccag atacagttcc ataaccatcc
    10021 tcaaaatgta ctttttgtct gaatgtagac tatcacggga aaactctctg aacagttgac
    10081 gcacatcaat ccaactttca ttcgaaagta ttttaatgtt tgaacgaaac agagaggcaa
    10141 ctatgatttt caatattcaa cgctactcga cccatgatgg ccccggtatc cgcacggtcg
    10201 tatttcttaa aggctgttcg ctgggctgcc gctggtgtca gaacccggaa agccgcgccc
    10261 gcacgcagga tctgctgtat gacccacgac tctgtctgga aggctgtgag ctgtgcgcta
    10321 agaccgcacc ggaagtgatt gagcgcgcgt tgaatggttt gcttattcat cgggaaaagt
    10381 taaccccgga gcattttacg gcgctaaccg actgctgccc gacacaggcg ttaaccgtgt
    10441 gtggtgaagt gaaaagcgtt gaggagatca tggcaaccgt tttgcgcgat aaaccgtttt
    10501 acgatcgcag cggcggcggc ttaacacttt ctggcggaga accctttatg cagccggaaa
    10561 tggcgacagc gctactgcaa gccagccacg aagccggtat tcatactgcg gtagaaacct
    10621 gtctgcatgt gccgtggaaa tatatcgccc cttctctgtc ctatattgat ctgtttcttg
    10681 ccgatttaaa acacgttgcc gacgcgccgt ttaaacagtg gaccgacggt aacgccgcca
    10741 gggtgctgga taatctgaaa aaactcgccg cagcgggcaa aaaaatcatt atccgcgtgc
    10801 cactgattca gggctttaat gccgacgaaa cctctgtgaa agccattacc gattttgccg
    10861 ccgacgagct gcacgttagc gaaattcatt tcctgcccta ccacacgctg ggcatcaaca
    10921 aatatcactt acttaatctg ccctatgacg ccccggaaaa accgcttgat gcgccagaac
    10981 tgctcgactt tgcccagcag tatgcctgcc agaaagggtt aaccgcgacc ttacgaggat
    11041 aacaaccatg accacactga aactggacac gctcagcgat cgcattaaag cgcacaaaaa
    11101 tgcgctggtg catattgtga aaccgccggt ctgtaccgag cgcgcgcagc actataccga
    11161 gatgtatcaa caacatctcg ataagccgat cccggtacgt cgcgcgctgg cactggcgca
    11221 tcacctggcg aatcgcacca tctggatcaa acatgatgaa ttgatcattg gcaaccaggc
    11281 aagcgaagtt cgcgccgcgc cgatcttccc ggaatatacc gtctcctgga tcgaaaaaga
    11341 gattgatgat ctggcagatc gtcccggtgc tggttttgcg gtgagtgaag agaacaaacg
    11401 cgttctgcat gaagtgtgcc cgtggtggcg cggtcagacc gtacaggatc gctgctacgg
    11461 catgtttacc gatgagcaaa aaggtctgct ggcgaccggc atcattaaag cggagggcaa
    11521 tatgacctcc ggcgatgcgc acctggcggt caatttcccg ctactgctgg aaaaaggtct
    11581 tgatggtctg cgtgagaaag tggcggaacg tcgctcgcgc atcaatctga cggttctgga
    11641 agatctgcac ggcgagcaat tcctgaaagc gattgatatc gtgctggtgg cagtcagtga
    11701 acacattgaa cgtttcgctg ccctggcgcg tgaaatggcc gcgaccgaaa cccgcgaaag
    11761 ccgtcgcgat gaactgctga cgatagcaga aaactgcgat cttatcgccc accagccgcc
    11821 gcagactttc tggcaggcgc tgcaactgtg ttacttcatc cagttgattt tgcagattga
    11881 atctaacggt cactcggtat cgtttggtcg tatggaccag tatctctatc catactatcg
    11941 ccgtgacgtt gaactgaacc agacactgga tcgcgaacac gccatcgaga tgctgcatag
    12001 ctgctggttg aaattgctgg aagtgaacaa aatccgctcc ggctcacact caaaagcctc
    12061 tgcgggaagt ccgctgtatc agaacgtcac cattggcggg caaaatctgg ttgatggtca
    12121 accgatggac gcggtaaatc cactctctta tgcgatcctc gaatcttgcg gtcgcctgcg
    12181 ttcgactcag cctaacctca gcgtgcgtta ccatgcgggt atgagtaacg atttcctcga
    12241 cgcctgtgtt caggtgatcc gctgcggctt cgggatgccg gcgttcaaca acgacgaaat
    12301 cgtcatcccg gaatttatta aactcggtat tgaaccgcag gacgcttacg actacgcagc
    12361 aattggttgt atcgaaaccg ccgtcggtgg caaatggggc tatcgctgta ccggcatgag
    12421 ctttatcaac ttcgcccgcg tgatgctggc ggcgctggaa ggcggtcgtg atgccaccag
    12481 cggcaaagtg ttcctgccac aagaaaaagc gttgtcagcg ggtaacttca acaacttcga
    12541 tgaagtaatg gacgcatggg atacgcaaat ccgttactac acgcgtaaat caatcgaaat
    12601 cgaatatgtc gtcgacacca tgctggaaga gaacgtacac gatattctct gctcggcgct
    12661 ggtcgatgac tgtattgagc gagcgaaaag tattaagcaa ggcggcgcga aatatgactg
    12721 ggtttctggc ctgcaggtcg gcattgctaa cctcggcaac agcctggcgg cagtgaagaa
    12781 actggtattt gaacagggcg cgattggtca gcaacagctg gctgccgcac tggcggatga
    12841 cttcgacggt ctgactcacg agcagctgcg tcagcggctg attaacggtg cgcctaagta
    12901 cggcaacgac gatgatactg tcgatacgct gctggctcgc gcttatcaga cctatatcga
    12961 cgaactaaaa cagtaccata acccacgcta cggtcgtggt ccggttggcg gcaactatta
    13021 cgcgggtacg tcgtctatct ccgctaacgt accctttggc gcgcagacta tggcaacacc
    13081 ggacgggcgt aaagcgcata ccccgctggc agaaggcgct agtccggcct ccggtactga
    13141 ccatctcggc ccaacggcgg tcattggctc agtaggtaaa ctgcctacgg ccgcgattct
    13201 cggcggcgtg ctgctcaacc agaaactgaa tccagcaacg ctggagaacg aatctgacaa
    13261 gcagaaactg atgatcctgc tgcgcacctt ctttgaagtg cataaaggct ggcatattca
    13321 gtacaacatc gtttcccgcg agacgctgct ggaggcgaaa aaacatccgg atcagtatcg
    13381 cgatctggta gtgcgcgtcg cgggctattc cgccttcttc accgcgctct ctccagacgc
    13441 tcaggacgat atcatcgccc gtactgaaca tatgctgtaa tcttctcagc ccggcggcgg
    13501 cgtcgccggg ctaaatcatt cacatcatca atttcagcct cgctttcatt cgaaatataa
    13561 tttgtgctct gcgtcacatt gttattacac tgttacggtt tcattttcga gccaggagca
    13621 cacctatgag cgtaaaagtt atcgtcacag acatggacgg tacttttctt aacgacgcca
    13681 aaacgtacaa ccaaccacgt tttatggcgc aatatcagga actgaaaaag cgcggcattg
    13741 agttcgttgt cgccagcggt aatcagtatt accagcttat ttcgttcttt cctgagctaa
    13801 aggatgagat ctcttttgtc gcggaaaacg gcgcactggt ttacgaacat ggcaagcagt
    13861 tgttccacgg cgaactgacc cgacatgaat cgcggattgt tattggcgag ttgctaaaag
    13921 ataagcaact caattttgtc gcctgcggtc tgcaaagtgc atatgtcagc gaaaatgccc
    13981 ccgaagcatt cgtcgcactg atggcaaaac actaccatcg cctgaaacct gtaaaagatt
    14041 atcaggagat tgacgacgta ctgttcaagt tttcgctcaa cctgccggat gaacaaatcc
    14101 cgttagtgat cgacaaactg cacataacgc tggatggcat tatgaaaccc gtcaccagtg
    14161 gttttggctt tatcgacttg attattcccg gtctacataa agcaaacggt atttcgcggt
    14221 tactgaaacg ctgggatctg tcaccgcaaa atgtggtagc gattggcgac agcggtaacg
    14281 atgcggagat gctgaaaatg gcgcgttatt cctttgcgat gagcaatgct gcggaaaaca
    14341 ttaaacaaat cgcccgttac gctaccgatg ataataatca tgaaggcgcg ctgaatgtga
    14401 ttcaggcggt gctggaaaat aaaccgccgt ttaatctttg attatcacca attcagttta
    14461 ttgagaaagt gcatgtcatt ataccggata cggcgtgacc gccttatttg gcatacaaaa
    14521 tcatgaaaat tcaatatatt gcgatacact accaggcctg ataagcgtag cgcaccaggc
    14581 aatcttgcgt ttgtcatcag tttcagtagc cctgaccata tcagggctac tgataataac
    14641 ttactcgatc cctttactac gcaggtaatc ttcgtaatta ccgctaaagt cgatcacgcg
    14701 ttccggggtg atttccagaa tacgggtcgc cagggagctt acaaactcac ggtcgtgaga
    14761 aacaaagatc agcgtgccct gatacagttc cagcgccatg ttcagcgact caatggattc
    14821 catatccagg tggttggtcg gttcgtccat gatcagaatg ttcggcttct gcatcattaa
    14881 cttaccaaac agcatccgcc ctttttcccc accggaaagc actttagctg gctttttgat
    14941 gtcgtcctgg ctgaacagca aacgaccgag aatactgcgc accgcctgct cgtcatcgcc
    15001 ttcctgcttc cactgactca tccattcgaa cacggtcaga tcattttcaa actcatattc
    15061 gtggtcctga gcgtagtaac caatgcgcgc gttttcagac cattttacgg tgccgctgtc
    15121 cggttgcaga tcgcccacca gcgttttcag cagcgttgat ttaccgacgc cgttggtacc
    15181 cagtaccgcc agtttttcac ccacttccag cagcaggttg agatttttaa acagtggacc
    15241 gttatcaaac cctttagtca gaccttccac ttctagcgcg ttacggaaca gtttcttatc
    15301 ctgttcaaaa cggatgaacg ggttctgacg gctggaagct ttcacctctt ccagtttgat
    15361 tttatcaatc tggcgcgcgc gcgaagttgc ctggcgagat ttcgaggcgt tggcgctaaa
    15421 gcggctaacg aaagattgca actcagcaat ctgcgctttc ttcttcgcgt tatcagccag
    15481 cagacgttca cgcgcctgag tcgccgccgt catgtactca tcgtagttgc ccggataaac
    15541 gcgcagctcg ccgtaatcca gatccgccat gtgggtacaa accatgttaa ggaagtgacg
    15601 gtcgtgcgag atgatgatca tggtgctgtc acgctcgttc agcacctgtt ccagccagcg
    15661 aatggtgtcg atatcgaggt tgttggtcgg ttcgtcgagc aggagaatgt cgggatcagc
    15721 aaacagcgcc tgagccagca gcacacgcag cttccagcca ggagcaactt cactcatcgg
    15781 gccgtagtgc tgttccactg gaattcccac gccaagcaac agttcaccag cgcgagcttc
    15841 cgcagagtaa ccgtccattt cgccgtattt cacttccaga tcggccactt tatagccgtc
    15901 ttcttcactc atttccggca aagcatagat gcggtcacgc tcctgcttca cttcccacaa
    15961 ctctttatgc cccatgatca ccgtatccag cacagtgaac tcttcaaagg caaactgatc
    16021 ctgacgcagc ttaccaatgc gttcgttggg atcgagggaa acgttaccca acgtcggctc
    16081 taagtcgccg ccgaggatct tcataaaggt ggatttacca ctaccgttcg cgccaatcag
    16141 gccgtaacgg ttgccgccgc cgaatttgac ggaaatgttt tcaaacaacg gcttactgcc
    16201 gaactgcatg gtgacgttac tggaaactaa cacaggcgta tcctgaaaag agatatgaca
    16261 aaccgcgcat tatgccataa gtctgaaaga agatcctgct cctcgcttaa ggtctacact
    16321 caagccaaag gcaaaaaatg tgatttcgta cacatctgat ttcactgtga gctggaatga
    16381 acttataatg cgcttccagt actctaatat tctcaaccca atggcctgcc aggcacaaaa
    16441 tctcgcttaa catgaatatg aaattgaaaa cattattcgc agcggccttc gctgttgtcg
    16501 gcttttgcag taccgcctct gcggtaactt atcctctgcc aaccgacggg agtcgcctgg
    16561 ttggtcagaa tcaggtgatc accattcctg aaggtaacac tcagccgctg gagtattttg
    16621 ccgccgagta ccagatggga ctttccaata tgatggaagc gaacccgggt gtggatacct
    16681 tcctgccgaa aggcggtact gtactgaaca ttccgcagca gctgatcctg ccggataccg
    16741 ttcatgaagg catcgtcatt aacagtgcag agatgcgtct gtattactat ccgaaaggga
    16801 ccaacaccgt tatcgtgctg ccgatcggca ttggtcagtt aggcaaagac acgcctatca
    16861 actggaccac caaagttgag cgtaaaaaag caggcccgac ctggacgccg accgctaaaa
    16921 tgcacgcaga gtaccgcgct gcgggcgaac cgcttccggc ggtcgttccg gctggtccgg
    16981 ataacccgat ggggctgtat gcactctaca tcggtcgcct gtacgctatc catggcacca
    17041 acgccaactt cggtatcggc ctgcgtgtaa gtcatggttg tgtgcgtctg cgtaacgaag
    17101 acatcaaatt cctgttcgag aaagtaccgg tcggtacccg cgtacagttt attgatgagc
    17161 cggtaaaagc gaccaccgag ccagacggca gccgttatat tgaagtccat aacccgctgt
    17221 ctaccaccga agcccagttt gaaggtcagg aaattgtgcc aattaccctg acgaagagcg
    17281 tgcagacagt gaccggtcag ccagatgttg accaggttgt tctggatgaa gcgatcaaaa
    17341 atcgttccgg gatgccggtt cgtctgaatt aatcttcaaa acgtaaagca aaggcggact
    17401 cataatccgc ctttttaatt tgacagacct tatcccgccg ggatcataac caacaaaaca
    17461 tatcccacca gcgccgccca caacagcatc ggtatcgaat aaagatgaaa acgccaccag
    17521 atacggcgat cgttcgccat ccgtaacgca atcaaatttg ccagcgatcc gggtaataaa
    17581 ccaaagccac ctacgtttac cgcccatgcc agtaataaag acggtggcac ataattgagc
    17641 agcaaaatgg tactcggcac gttactgatc acctgcgata aaccgatcgc cgttagccat
    17701 aatccgggtt cggataaatg actcacgtta cttaacaccc cctgcaaaac tggcagttgg
    17761 gttagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
    17821 ctgagcacca cgcgacgcgc cagcagcgca aagcccgccg ccacaatcac cagtccccac
    17881 agctcttgtt ttaactccag cgccgtcaga aagacgatat acagccccag acaactccac
    17941 accagccgcg gtttccactc cggcgtttgc cctcctgtat ggtactgcag cgcctttcca
    18001 gggaaacaac accagcacaa cagcaggagt gtcagcatca ttgcgccagc cagcggtgcc
    18061 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
    18121 gggttgccaa ttggcgtcag tagcgaacca gcattgactg ccagcgcctc aaaaataatc
    18181 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaactata
    18241 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag taccataaac
    18301 atcgccagcc gacgctccgt agcaaaacgg cgcaccattt tgcgccccag cacatcaaaa
    18361 taaccgctta actcgacacc tttagtcagc agcatcaggc cgcttaaggt gatgatggtg
    18421 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gaacgaaaaa acttaatccg
    18481 atgccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
    18541 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa ggcatccagc gtctcttcac
    18601 taacatggtg ctccatacct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
    18661 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
    18721 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
    18781 gtcgcttaag cattttagcc accgtcggtt gcgaaacccc cagacgagca gccatgtcta
    18841 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaaca taatcatcaa
    18901 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
    18961 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attatgcttc
    19021 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
    19081 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
    19141 tgtatgtaat gcaatcatcc ctcaaggatc gatgggatta gcaagtcagg aggtcttatg
    19201 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
    19261 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaggataa accttccgat
    19321 aaatagcggc gtcgcggtac gccgcttcac tcctgctttt atgcaggcac aacgcgtttt
    19381 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
    19441 cggtagttga cctgaacagc agctcgctct atcttcttgc agccctgcgt atattgcggc
    19501 tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga cccttgacgc
    19561 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
    19621 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
    19681 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
    19741 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag
    19801 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa
    19861 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
    19921 tccgtggtac agctacctgg tcggcatatt tatttttgcg ctcggcgtaa tagcaatcag
    19981 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gcctggtatt
    20041 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggcg gcgtatttaa
    20101 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
    20161 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
    20221 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggag aaagcgcgcg
    20281 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
    20341 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
    20401 cttaacgctc aatcgcgttg tcgatggcaa accacagttt caggataact ttgtcaccct
    20461 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggtcaaa tcggcgaata
    20521 cgataccgcc atcgccagca tcgccaaacg agcagatgaa gtgtacttcc tgaaagaagg
    20581 taattttgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga ccgctcaagt
    20641 gctggcgcaa gagcactcgc aaccgcagct gattgttcta catctgatgg gctcgcatcc
    20701 gcaggcctgc gaccggacac aaggaaaata cgaaaccttt gtgcaatcga aagaaacgtc
    20761 gtgctatctc tataccatga cgcaaacaga cgatttactg cgcaagctgt acgatcagtt
    20821 acgcaacagc ggcagcagct tctcgctggt ttacttttct gaccacggtc tggcctttaa
    20881 agagcgcggc aaagacgtgc aataccttgc tcatgatgat aagtatcagc aaaatttcca
    20941 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
    21001 ctcggccaat gacttcttag gctttttctc gcagtggacg ggaattaaag cgaaggaaat
    21061 aaatatcaaa tacccgttta tatctgaaaa gaaagccggg ccgatataca tcaccaactt
    21121 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaaacctta
    21181 aaacaaaaaa atccgccccg agaggcggat ttttatatca ccgaagtgat tagaagcggt
    21241 aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag
    21301 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
    21361 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac
    21421 ccacacccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
    21481 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
    21541 tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
    21601 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
    21661 ttacagtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
    21721 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
    21781 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tatgatgtct aacgggccat
    21841 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
    21901 agaactgcgt aagtattacg catattttcc ctgtcattca tccagattat tcctaatcac
    21961 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
    22021 acaaatgctc aaccagcatt gggtatatcc agtacactcc acgctttact taggtttaga
    22081 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaatag
    22141 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
    22201 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattaggc acgctgatcc
    22261 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacga ttaccgctgt
    22321 tgttttacgg cgtttcgctg ggtggaatga attatctttt ttatctttct attcagacgg
    22381 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcactgttct
    22441 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
    22501 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
    22561 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
    22621 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtc ccaattggag
    22681 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggg ctggctgtcg
    22741 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
    22801 cacgcacatt tggtacgcta atgagcatgg aaccggctct ggctgccgtt tccgggatga
    22861 ttttcctcgg agaaacactg acccccatac agctgctggc gctcggcgct atcatcgccg
    22921 cttcaatggg ttctacgctg acagtacgca aagagagcaa aataaaagaa ttagacataa
    22981 attaaattta catttctgca tggttatgca taaccatgca gattttctcg ctacttttcc
    23041 tctacaccgt ctttatatat cgaattatgc a
//
LOCUS       DACADF010000075        22179 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.075, whole genome shotgun sequence.
ACCESSION   DACADF010000075 DACADF010000000
VERSION     DACADF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.075"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            75..290
                     /gene="bdm"
                     /locus_tag="ISB30_002733"
     CDS             75..290
                     /gene="bdm"
                     /locus_tag="ISB30_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HAP1990348.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            392..529
                     /gene="sra"
                     /locus_tag="ISB30_002734"
     CDS             392..529
                     /gene="sra"
                     /locus_tag="ISB30_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HAP1990349.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            685..2382
                     /locus_tag="ISB30_002735"
     CDS             685..2382
                     /locus_tag="ISB30_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP1990350.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            2516..3526
                     /gene="adhP"
                     /locus_tag="ISB30_002736"
     CDS             2516..3526
                     /gene="adhP"
                     /locus_tag="ISB30_002736"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAP1990351.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATKMGADLAINSRTEDA
                     AKIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLEDINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3712..3989
                     /locus_tag="ISB30_002737"
                     /pseudo
     CDS             3712..3989
                     /locus_tag="ISB30_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Killer protein"
     gene            3989..4273
                     /locus_tag="ISB30_002738"
     CDS             3989..4273
                     /locus_tag="ISB30_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAP1990352.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4323..4976)
                     /locus_tag="ISB30_002739"
     CDS             complement(4323..4976)
                     /locus_tag="ISB30_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAP1990353.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4969..5853)
                     /gene="fdxH"
                     /locus_tag="ISB30_002740"
     CDS             complement(4969..5853)
                     /gene="fdxH"
                     /locus_tag="ISB30_002740"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAP1990354.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDEEEGNHE"
     gene            complement(5866..8913)
                     /gene="fdnG"
                     /locus_tag="ISB30_002741"
     CDS             complement(5866..8913)
                     /gene="fdnG"
                     /locus_tag="ISB30_002741"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8326..8328),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAP1990355.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9145..10026
                     /gene="yddG"
                     /locus_tag="ISB30_002742"
     CDS             9145..10026
                     /gene="yddG"
                     /locus_tag="ISB30_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAP1990356.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAVYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10299..11687
                     /locus_tag="ISB30_002743"
     CDS             10299..11687
                     /locus_tag="ISB30_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HAP1990357.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            11782..15522
                     /locus_tag="ISB30_002744"
     CDS             11782..15522
                     /locus_tag="ISB30_002744"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAP1990358.1"
                     /translation="MSKLLDRFRYFKQKGEIFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFAFDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLSLKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            15519..17063
                     /gene="narH"
                     /locus_tag="ISB30_002745"
     CDS             15519..17063
                     /gene="narH"
                     /locus_tag="ISB30_002745"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAP1990359.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            17063..17758
                     /gene="narW"
                     /locus_tag="ISB30_002746"
     CDS             17063..17758
                     /gene="narW"
                     /locus_tag="ISB30_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAP1990360.1"
                     /translation="MQILKVLGLLMEYPDELLWECKDDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILTLLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            17755..18435
                     /gene="narI"
                     /locus_tag="ISB30_002747"
     CDS             17755..18435
                     /gene="narI"
                     /locus_tag="ISB30_002747"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAP1990361.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            18515..19408
                     /locus_tag="ISB30_002748"
     CDS             18515..19408
                     /locus_tag="ISB30_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HAP1990362.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRSKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLTGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKSETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIDF"
     gene            complement(19504..20349)
                     /gene="nhoA"
                     /locus_tag="ISB30_002749"
     CDS             complement(19504..20349)
                     /gene="nhoA"
                     /locus_tag="ISB30_002749"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HAP1990363.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVIGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            20524..21093
                     /locus_tag="ISB30_002750"
     CDS             20524..21093
                     /locus_tag="ISB30_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP1990364.1"
                     /translation="MSQFIPVELHHASCLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEGNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGMFVTSGKRVTAG"
     gene            complement(21164..>22179)
                     /locus_tag="ISB30_002751"
     CDS             complement(21164..>22179)
                     /locus_tag="ISB30_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HAP1990365.1"
                     /translation="EGWEDIEDFGETHLDFLKQYGDFDNGIPVHDTIARVVSCISPAK
                     FHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVIGQI
                     RTDEKSNEITPIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGR
                     LNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKK
                     LCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVM
                     NEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGS
                     GLS"
BASE COUNT         5255 a         5795 c         5670 g         5459 t
ORIGIN      
        1 tgccatgctg aatgtgcttt ataaatctgc gatccgtagc agacaccata aatacacaga
       61 cacggagaat cactatgttt acttattatc aggcagaaaa ttcaacagca gaacccgctc
      121 tggttaatgc cattgagcaa ggccttcggg cagagcacgg tgttgtcact gaagatgaca
      181 ttctcatgga gctgaccaaa tgggtggaag cttctgataa cgacatcctc agtgatatct
      241 accagcaaac gatcaattat gtggtcagtg gtcagcaccc tacgctttaa ggtgctatgc
      301 ttgatcggca acctaattta ggggtttagc acgtgtttct tcgctacggc gatgttgtcc
      361 ttaaaactag caacaggatt gaggagttaa aatgaaatcg aaccgtcagg cacgtcatat
      421 tcttggactg gaccataaaa tttctaacca gcgcaaaata gttaccgaag gtgacaaatc
      481 cagcgtggta aataatccca ccggcagaaa acgccccgct gaaaagtaat tcataaccat
      541 cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg gtgtttttgt ttttatctgc
      601 tttatacttg gtgccgacgc cctggcggta atgcaaagac gataaaagcc cccagggatg
      661 gatattcaaa aaagagtgag tgacatggaa ccaaaaacaa aaaaacagcg ttcgctttat
      721 atcccttacg ctggccctgt attgctggaa tttccgttgt tgaataaagg cagcgccttc
      781 agcatggaag aacgccgtaa cttcaacctg ctggggttac tgccggaagt ggtcgaaacc
      841 atcgaagaac aagcggaacg agcatggatc cagtatcagg gattcaaaac cgaaatcgac
      901 aaacacatct acctgcgtaa catccaggac actaacgaaa ccctcttcta ccgtctggta
      961 aacaatcatc ttgatgagat gatgccagtc atttacaccc caaccgtcgg cgcagcctgt
     1021 gagcgttttt ctgagatcta ccgccgttca cgcggtgtgt ttatctctta ccagaaccgc
     1081 cacaatatgg acgatattct gcaaaacgta ccaaaccata atatcaaagt gattgtggtg
     1141 actgacggcg aacgtattct ggggcttggt gaccagggca tcggcgggat gggcattccg
     1201 atcggtaaac tgtcgctcta taccgcctgt ggcggcatca gtccggccta tacccttccg
     1261 gtggtgctgg atgtcggcac caacaaccaa cagctgctta acgatccgct gtatatgggc
     1321 tggcgtaatc cgcgtatcac tgacgatgaa tactatgaat tcgttgatga atttatccag
     1381 gctgtgaaac aacgctggcc ggacgtactg ttgcagtttg aagacttcgc acaaaaaaat
     1441 gcgatgccgt tacttaaccg ctatcgcaat gaaatttgtt cttttaacga tgacattcag
     1501 ggcaccgcgg cggtaacagt cggcacactg atcgcagcca gccgcgcggc aggcggtcag
     1561 ttaagcgaga aaaaaatcgt cttccttggc gcaggttcag cgggatgcgg cattgccgaa
     1621 atgatcatcg cccagaccca gcgcgaagga ttaagcgagg aagcggcgcg gcagaaagtc
     1681 tttatggtcg atcgctttgg cctgctgacc gacaagatgc cgaacctgct gcctttccag
     1741 accaaactgg tgcagaagcg cgaaaatctc agtgactggg ataccgacag cgatgtgttg
     1801 tcactgctgg atgtggtgcg taatgtaaaa ccagatattc tgattggcgt ctcaggacag
     1861 accgggctgt ttacggaaga gatcatccgt gagatgcata aacactgtcc gcgtccgatc
     1921 gtgatgccgc tgtctaaccc gacttcacgc gtggaagcca caccgcagga cattatcgcc
     1981 tggactgaag gtaacgcgct ggtcgccact ggcagtccgt ttaatccagt ggtatggaaa
     2041 gataaaatct accctatcgc ccagtgtaac aacgccttta ttttcccggg catcgggctg
     2101 ggtgttattg cttccggcgc gtcacgtatc accgatgaga tgctgatgtc ggcaagtgaa
     2161 acacttgctc agtattcgcc gctggtcctg aacggcgaag gtctggtact accggaactg
     2221 aaagatattc agaaagtctc ccgcgcaatt gcctttgcgg ttggcaaaat ggcgcagcag
     2281 caaggcgtgg cggtgaaaac gtctgccgaa gctctgcaac aggccattga tgataatttc
     2341 tggcacgccg aataccgcga ctaccgccgt acctccatct aagcctgcgc ccggtagtga
     2401 gggctaccgg gcaattttcc tctccctttt tcagatctca tccatactgg gtagtggcga
     2461 ataaatctca tttgcctcac ctgctatgca gaacatcatc cgaaaaggag gaactatgaa
     2521 ggctgcagtt gttacgaagg atcatcatgt tgacgttacg gataaaacac tgcgctcact
     2581 gaaacatggc gaagccctgc tgaaaatgga gtgttgtggt gtatgtcata ccgatcttca
     2641 tgttaagaat ggcgattttg gtgacaaaac cggcgtgatt ctgggacacg aaggtattgg
     2701 tgtggtggca gaagttggcc ctggtgttac ttcattaaaa ccaggcgatc gtgccagcgt
     2761 ggcgtggttc tatgaaggat gcggtcattg cgaatactgt aacagtggta acgaaacgct
     2821 ctgccgttca gttaaaaatg ccggatacag cgttgatggc gggatggcgg aagagtgcat
     2881 cgtggtcgcc gattacgcgg taaaagtgcc agatggtctg gactcggcgg cggccagcag
     2941 cattacctgt gcgggggtca ctacctacaa agccgttaag ctgtcaaaaa ttcgtccagg
     3001 acagtggatt gctatctacg gtcttggcgg tctgggtaac ctcgccctgc aatacgcgaa
     3061 gaatgtcttt aacgccaaag tgatcgccat tgatgtcaat gatgaacagt taaaactggc
     3121 aaccaaaatg ggtgcagatt tagcgattaa ctcacgcacc gaagacgccg ccaaaattgt
     3181 gcaggagaaa gccggtggcg ctcacgcagc ggtggtgaca gcggtagcta aagctgcgtt
     3241 taactcggcg gtggatgctg tccgtgcagg tggtcgtgtc gtggctgtcg gtctgccgcc
     3301 ggagtctatg agcctggaca tcccacggct ggtgctggat ggtattgaag tggtcggttc
     3361 gctggtcggc acgcgtcagg atctaaccga agccttccag tttgccgccg aaggcaaagt
     3421 ggtgccgaaa gtcgccctgc gtccgttaga ggacatcaac accatcttta ccgagatgga
     3481 agaaggcaaa atccgtggcc gtatggtgat tgatttccgt cgctaagagg tctttgctgc
     3541 gactgccatg ttcgggtcgc agcctgtttc gcgagacgct ttccgtaatt tcactcttct
     3601 tcttttcctc ccccatattt cccctcacaa ttcgcgaaaa cgtagcgaaa tttgttgcgc
     3661 tgcacggaac aatagtgata gtgttccttt tgagggaaca aggatgaaga gatgataatg
     3721 aatttcagac acaagggatt gcgtgacttg tttcttctcg gcaaaacctc cggcgttata
     3781 ccgacgcaag tcaaacgatt acgccaccac tcgctgtgat tgatgcagca tgctgtcttg
     3841 ctgatatcga tatgcccggt taccgactac atccgttaag cggcgatcgc gatggaattt
     3901 gggcgatatc tgtctcgggc aactggcgaa tcacatttga attcgtcaat ggcgatgcat
     3961 acatactgga ttacgaggac tatcactgat gaaaatggcc aatcatcccc gcccggggga
     4021 cattattcag gaatcactgg acgaacttaa tgtcagcctg cgcgagtttg ccagagcaat
     4081 ggaaattgcg ccctcaacgg caagccgatt gctgaccgga aaagcagctt tgacgccaga
     4141 aatggcaata aaactttccg tggtgatcgg cagttcgccg caaatgtggc tgaatctgca
     4201 aaatgcctgg agtctggcag aggcggaaaa aacggtggat gtgtcgcgac tgcgccgtct
     4261 ggttacgcaa taaaaaatgg cgcttcttaa agcgccattt tcagcttaaa gttcaaaaga
     4321 gattatatcc cttcttcact ctcttttttc gcttctgcct tctcgatttc acgataccag
     4381 cgcggatggt gtttcttcgc ccagcgacgg ctcaccttcc cttcgatcat ccctttaatc
     4441 gatcctttca cccagaatgc catatacata tggatcagga tggcgtggat caggatgata
     4501 cccgcagccg catggatcag caggctataa cggacaacct gtatcgggaa gtactgcgca
     4561 aagtacggac gccagataat cactccggtc accagcagca cgaaaatcat gctcatgatt
     4621 gaccagaaca tcatcttttg cccggcgttg tacttaccga catccgccac tttgtgctca
     4681 ttgcctttta atacttcgac aatgttcaac agccacggaa tatctttctt atccgggatg
     4741 ttgtgatgca caaaacgcac aaacataaac atcagcgcga cgaaaatcgc aatgccgaag
     4801 aacgggtgca aaatgcgtcc catctgcggc gtaccgaagg tttgcgtcag ccattgcagc
     4861 gtcgggaaga agaacgaaat cccggacagc gccaccagga agaagcaaat caccacggtc
     4921 cagtgacagg cgcgatcaat aaatttggtg cgcacaatca ttttcgactt actcatgatt
     4981 tccctcctcc tcatcgtcca cttccttatt cgggccaata ccgatgtagt ggaaaatcaa
     5041 cccggcaaaa gtggcaataa agccagccgc tgccagcggt ttcaacgcgc ctttccacag
     5101 gcttaccgag gtatcgatct tcggatcttt cggcagacca tgatacagct ccggctgatc
     5161 ggcgtgatgc agcacgtaca taacgtgcgt accaccgacc ccttccgggt tgtagacgcc
     5221 agcatgttcg taaccacgcg ctttcagttt cgccacgcgc tgttccgcca gctccagcat
     5281 ctccttcttg gtgccgaagt ggatagcccc ggtcggacag gttttcacac aagccggttc
     5341 ctggccgacg ctgacgcgat cgacacagag cgtgcattta tatacccggt tatcctcttt
     5401 gttgaggcgc ggaatattaa acggacaccc ggcaatgcag taaccacagc cgatgcagtt
     5461 ttccgactgg aaatcgacaa tcccgttagc gtactgaatg attgcaccag cagacgggca
     5521 tgccttcagg cagcccggat cttcacagtg catacagccg tctttacgga tcagccactc
     5581 cagcttgccg ttctgttcgg tttcgctaaa gcgcatcacc gtccaggact tggcgctcag
     5641 atcggcgggg ttatcgtaaa ccccgacgca gtgccccact tcatcacgga tgtcgttcca
     5701 ctccgaacac gccacctgac aggctttaca gccgatacaa gtggaaacgt cgataagttt
     5761 cgcgacttct gctttgtaat cacgcacctg agaaggcggc gtgatggagt tagttgcgga
     5821 ccttttgata atgtcctgcg tttccatagc catctgttcg cccccttacg ccttctcgat
     5881 gtttactaag aacgctttat attccggcgt ttgcgagttt gcatcaccga cattcggcgt
     5941 cagagtgtta gcgatataac ctttacgcgc gacaccttca aagccccagt ggattggaat
     6001 acccaccgtt tcaacctgct gaccgtttac attcagcggt ttcagacggc gcgttaccac
     6061 agccaccgcg cggataaagc cacgcttgct ggagacagtg acacgatcgc cattggcaat
     6121 gcctttcgcc gccgccagcg tttcgctgat ttccacaaac tgttccggct gagcaattgc
     6181 gttgagcaat gcgtgcttgg tccaggtgtg gaagtgctcc gtcagacgat aggtcgtacc
     6241 cacatacggg aactgctctt ttttacccat ccgcagcgcg tcttgttcat acagacgaac
     6301 aaccgggtta gacaccacgt tcggatgcaa cgggttagtg cccagcggcg tttcaattgg
     6361 ctcgtagtgt tccgggaacg gaccttccgc cattttgttg atggcaaaca ggcgtcccat
     6421 cccttccggt tgcatgataa acggcccggt tggcgtaccc ggcgcggcat tgccgaagtc
     6481 cggaatatcg ttacccgtcc acttgctacc gttccactgg atcagcatcc gtttcggatc
     6541 ccacggttta ccgttgatat ccgccgaagc acggttgtag agtacgcgac ggttgagcgg
     6601 ccacgcccag gcccatccca gcgtattccc cagaccggac gggtcggagt tatcgcggtt
     6661 agccatctgg ttgccctgct cggtccagct accggtgtag atccagcaag aagatgcggt
     6721 tgtaccgtca tcacgcagat gtgcaaagct actcagcaac tgacctttct tcgcaatcag
     6781 cacgccatta gcgtcataga gatcttccag cgcatagccg ttattctctt tcgccacttc
     6841 atcagactgc ggttcgtgcg gctgcttgta gttccagctc atcttcatca gcggttctac
     6901 gcctttacca ccttcggcct ggtacagctc gcgcagatga tggtagatac ccgccagaat
     6961 ttcgccgtcg ttacgtgctt cgcccggcgc gtcctgacct ttccagtgcc actgcaacca
     7021 gcgaccggag ttagcgatag aaccatcttc ttcagcaaag caggtcgaag gcagacggaa
     7081 tacttcagtc tgaatagatg ccggatcgac atcgttcgac tcgccgtggt tctgccagaa
     7141 ggtagaggtt tcagtcacca gcggatcgat aaccaccatg tacttcagct tgctcaggca
     7201 gctcaccact ttgtttttgt ccgggaagga cgcaaccggg ttaaagccct ggcagaaata
     7261 accggtgact ttaccttcat ccatcatgtt gaaatacttg atgacgtcgt aggtctggtc
     7321 ccacttcggc agccagtcgt agccccagtt gttctctttc tgcgcggcat cgccgtagaa
     7381 agatttcatc aggctaacga aaaacttcgg atagttgctc cagtagttca cctgatcagc
     7441 cagcgtcgct ttcggcgtgt tcgcttccag atacgactgc aaatcaacct gtttttctga
     7501 cggcagcgtc agataacctg gcaggctggt agagagcagg cctaagtcag tcaggccctg
     7561 aatgttggag tgaccacgca atgcgttcac gccgccaccg gccataccca tgttaccgag
     7621 cagcaactgg atcatcgcca tcgtacggat gttctgcgca cccacggtgt gctgcgtcca
     7681 gcccagcgcg tacaggaagg tggttgtgcg atccggtgcg ctggtggagg ccagcacttc
     7741 acacactttc aggaagtcgg cttttggcgt accgcaaatg ttttctacta cgtccggcgt
     7801 gtagcgggaa acgtgcgctt tcagcagatt ccatacacag cgcggatgag tcagcgtgtc
     7861 atcgcgtttc gcatagccgt tttcatcgaa ctgatagttc caggacgact tatcgtattg
     7921 acgtttttca gcgtcgtagc cgctgaacag accgtcttcg aaagcaaaat catcacgcac
     7981 cagcaggctg gcgttggtgt aatgcttaac gtattcggcg ttgattttgt tgttttcgat
     8041 caggtagcgc aaaacgccag acaggaacgt aatgtccgta ccggaacgaa taggtgcgta
     8101 aatatccgcc acagaagcgg tacgcgtaaa acggggatcg acaacgatca aggttgcatc
     8161 gttgttgttt ttcgcttcca tcgcccagcg gaaaccgacg ggatgcgctt cagcagcgtt
     8221 accgcccatc accattacga cgttagcgtt tttgatatcc acccagtggt tggtcatcgc
     8281 accgcgacca aatgttggag caagacttgc taccgttggt ccgtgtcaga cgcgcgcctg
     8341 gttgtctacc gccagcatcc cgagggagcg ggcaaatttt tgagtcagca tcccggtttc
     8401 gttgctggca ccggaagcac acagcatacc ggtagaaagc caacggttta ccgttacgcc
     8461 ctgctcgttc ttttcaataa agttagcgtc acggtcggct ttcatcagct tcgcaatacg
     8521 ggagaatgct tcttcccagc taatgcgctg ccatttgtca gaacctggcg cacgatattc
     8581 cgggtagcgc agacggtttt cgctgttgac ataatccagc aaaccggccc ctttcgggca
     8641 cagcgcacca cgacttaccg gatgatccgg gtcaccttca atgtgataaa tcgcttctct
     8701 ggcgtttttc gcgccatcac ccaggctata catcaatagc ccgcaaccta cggaacagta
     8761 tgtgcaggtg ttacggatct ctttcgcgcg taataatttg tagtttcgcg cctgagccag
     8821 tgcttgcttc ggggcaaagc ccaatgctgc tactgttgtt cccgccatac cgcccgcgca
     8881 gattttaaaa aattgtctgc gactgacgtc cattgctttc ctcttttttc aggggtatta
     8941 ctgcgcgagg aaactaacag taaaattctg aatctttttg cgcgaaatca aaaagaacac
     9001 gtgtttgcca caaaataatc cgcctgcgca aattgaccta cctcaatagc ggtagaaaaa
     9061 cgcaccactg cctgacaggc cagttaaaaa aatgctataa aattcagctt aatttttaac
     9121 ggcaagagag acaaaacagc gagcatgaca cgtcaaaaag caacgctcat agggctaata
     9181 gcgatcgtcc tgtggagcac gatggtagga ttgattcgcg gtgtcagtga gggactcggc
     9241 ccggtcggcg gcgcagctgc tatctattca ttaagcgggc tgctgttaat cttcacggtt
     9301 ggatttccgc gtattcggca gatcccgaaa ggctatttac tcgccgggag tctgttattc
     9361 gtcagctatg aaatctgtct ggcgctttcc ttagggtatg cggcgacccg tcatcaggcg
     9421 attgaagtgg ggatggtgaa ctatctatgg cccagcctga caattctctt tgccattctg
     9481 tttaatggtc agaaaaccaa ctggctgatt gtaccaggat tattattagc cctggtcggc
     9541 gtctgctggg tgttaggcgg tgacaatggg ttacattatg atgaaattat caataatatc
     9601 accaccagcc cattgagtta tttcctggcg ttcattgggg cgtttatctg ggcagtctac
     9661 tgcacagtaa cgaataaata cgcacgcgga tttaacggaa ttaccgtttt tgtcctgcta
     9721 acgggagcaa gtctgtgggt ttactatttt cttacgccac aaccagaaat ggtatttagc
     9781 acgcccgtca tgattaaact catctctgcg gcatttacct taggatttgc ttatgctgca
     9841 tggaatgtcg gtatattaca tggcaacgtc accataatgg cggtaggttc gtattttacg
     9901 cccgtccttt cctcagcgct tgcagccgtg ctgctcagcg ccccgctgtc gttctcgttc
     9961 tggcaaggcg cgctaatggt ctgcggcggt tccctgctct gctggctggc gacacgtcgt
    10021 ggctaaaata attgtcgggt ctacggcccg gcagttaatc agataaattc tgacaatatt
    10081 aaaaaataaa tttcaaatac cagaaagtca ttttagttac atttagtaac acatatctgt
    10141 ctgtttaatc cacattctgc ccccgcagtt acgctttctt atctattctt ctaatcagca
    10201 cttcacctga ttcatcctta attctaataa ctggctattg cgccttacct cctgttttgt
    10261 ctttttttca ttaccaatgt gtgcatgtga ggaacaatat ggcactgcaa aatgagaaaa
    10321 atagtcgtta tcttttacgc gactggaaac cagaaaatcc ggccttctgg gaaaataaag
    10381 gaaagcatat tgcacgaaga aacctctgga tatcagtcag ttgtctactt cttgccttct
    10441 gtgtctggat gctatttagc gcagttactg ttaatctcaa taaaatcggt tttaatttta
    10501 ctaccgatca actcttttta ttaaccgcat taccctccgt ttctggcgca ttattgcgtg
    10561 tcccctactc ctttatggtg cctatattcg gtggacgccg atggacggtt tttagtactg
    10621 caatcctgat tattccttgc gtctggctcg gaattgccgt gcagaatccg aatactcctt
    10681 ttgggatatt tatcgttatc gctttgctat gcggttttgc aggtgcgaac tttgcttcga
    10741 gcatgggcaa tatcagtttc ttctttccaa aagccaaaca agggagcgct cttgggatta
    10801 atggcggatt aggaaactta ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt
    10861 ttgtccccgt atttgccttt ctcggcgtca atggcgtacc gcaggccgac ggttcggtga
    10921 tgtcgctggc gaatgccgca tggatttggg tgccattact ggcgattgcc acgatcgccg
    10981 cctggtcagg gatgaatgat atcgccagtt cacgcgcctc aattgccgac cagctccctg
    11041 tcttacaacg cctgcatctc tggctgctga gcctgcttta ccttgccacc ttcggttcgt
    11101 ttatcggttt ttctgcgggt tttgccatgc tggcaaaaac ccagttcccg gatgtgaata
    11161 ttctgcgcct ggcgttcttt ggcccattta tcggtgccat cgcgcgatcg gttggtggtg
    11221 ctatttccga taaattcggc ggcgtgcggg tgacgttgat caacttcatt tttatggcga
    11281 ttttcagcgc cctgctgttc cttaccttac cgggcacagg ctccggtaat ttcatcgcat
    11341 tttacgccgt atttatgggg ctgtttctga ccgcgggtct gggaagtggt tctactttcc
    11401 agatgatcgc cgtcatcttt cgccagataa caatttatcg ggtgaagatg aaaggcggta
    11461 gtgatgagca agctcaaaga gaagccgtca ccgaaacggc agccgctctg ggctttatct
    11521 cagccattgg cgcagtgggc ggctttttta ttccgcaggc gtttggcatg tcgctcaata
    11581 tgaccggctc tccggtgggc gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc
    11641 tactgacctg gctggtttat ggtcggcgga agttcagcca aaaataagag atcgatgcct
    11701 gacacgtctt atcaggctac aaccgtgtcg catccgacaa ggcatcagat cccaattcat
    11761 tcctggagca ggagttatgt catgagtaaa cttttggatc gctttcgcta cttcaaacaa
    11821 aagggcgaaa tctttgccga tggtcacgga caggtgatgc atagcaaccg cgactgggag
    11881 gacagctatc gccagcgttg gcagttcgac aaaatcgtgc gttccaccca cggtgttaac
    11941 tgtacaggct cctgtagctg gaaaatctac gttaaaaatg gtctggtgac ctgggaaatc
    12001 caacagaccg attacccgcg cactcgccct gacctgccga atcacgaacc tcgcggctgc
    12061 ccgcgtggcg caagttactc ctggtatctc tacagcgcga accgcctgaa atacccgctc
    12121 attcgtaaac gactgattga actgtggcgc gaagccctca agcaacacag cgatccggta
    12181 ctggcgtggg catcgattat gaacgatccg caaaagtgcc tgagctacaa acaagtgcgt
    12241 ggacgcggtg gttttatccg ctccaactgg caggagctaa atcagctgat tgccgccgct
    12301 aacgtctgga ccatcaaaac ttacggcccg gatcgcgttg ccggtttctc gccgattccg
    12361 gcgatgtcga tggtttctta cgctgccgga acacgctatc tgtcgctgtt aggtggcacc
    12421 tgtttaagct tctacgactg gtattgcgac ctgccgcccg cctcgccgat gacctggggc
    12481 gagcaaaccg acgtaccgga atctgccgac tggtataact ccagttacat catcgcctgg
    12541 gggtctaacg taccgcagac acgtacgccg gacgcccatt tctttaccga agtacgctac
    12601 aaaggcacta aaaccatcgc cattacccct gactactctg aagtggccaa attgtgcgac
    12661 cagtggctgg caccgaaaca aggcactgat agtgccctgg caatggcaat gggccatgtc
    12721 attttaaaag agtttcatct cgataatccc agcgattatt ttatcaacta ctgccgccgc
    12781 tacagcgaca tgccgatgct ggtaatgctg gaacctcgcg acgacggcag ctacgttccc
    12841 gggcgcatgg tccgcgcatc tgacctggtg gatggactgg gcgaaagcaa taatccgcag
    12901 tggaaaaccg tggcagttaa taccgcaggt gaattggtag tgccgaacgg ttcgattggt
    12961 tttcgttggg gagaaaaagg caaatggaac ctggaatcca ttgccgccgg tacggaaacc
    13021 gaattgtcgt taaccctgct cggtcaacat gacgccgttg caggcgtggc cttcccctac
    13081 tttggaggca ttgaaaatcc gcattttcgc agcgtaaaac acaatcctgt cctggtgcgc
    13141 caattgcccg ttaaaaatct gacattagcc gatggtagca cctgtccggt ggtcagcgtt
    13201 tatgatctgg tactggcaaa ttacggcctc gatcgcgggc tggaagatga aaacagcgcg
    13261 aaagattacg ctgaaatcaa accttacacc cctgcctggg gcgagcaaat taccggcgta
    13321 ccgcgccagt atattgaaac catcgcccgt gaatttgccg ataccgctca taaaacgcat
    13381 gggcgctcga tgattattct cggcgcaggt gttaaccact ggtatcacat ggacatgaac
    13441 taccgcggga tgatcaatat gctgatcttc tgcggttgtg tcgggcaaag cggcggcggc
    13501 tgggcgcact atgtcggtca ggaaaaactg cgcccacaaa ccggctggtt gccgctggcc
    13561 tttgcgttcg actggaaccg tccaccgcgc cagatgaaca gcacctcgtt tttctacaat
    13621 cactccagcc agtggcgcta tgaaaaagtc accgcgcagg aattgctctc accgctcgcc
    13681 gatgccagta aatacagcgg tcacctgatt gatttcaacg ttcgcgccga acgtatgggc
    13741 tggctccctt ctgcgccgca gctggggcgt aacccgctct ccctgaaagc tgaagccgac
    13801 aaggccggat tatcccccac agaatttacc gcccaggcgc tgaaatcggg cgatttacgt
    13861 atggcctgcg aacaaccaga tagcggcagc aatcatccgc gtaatttgtt tgtctggcgt
    13921 tctaacctgc ttggctcctc cggcaaaggc cacgagtata tgcagaagta tctgctgggg
    13981 accgaaagcg ggattcaggg agaggaactc ggtgccagcg acgggatcaa accagaagaa
    14041 gtcgagtggc aaactgcggc gattgaaggc aagctcgacc tgctggtgac gctcgatttc
    14101 cgcatgtcca gtacctgcct gttctccgat atcgttctgc ccaccgccac ctggtacgaa
    14161 aaagacgata tgaatacctc ggatatgcat ccgtttattc atccgctttc tgcggcggtc
    14221 gatccggcgt gggaatcacg cagcgactgg gaaatctaca aaggtatcgc taaagccttt
    14281 tcgcaagtgt gcgtggggca tctcggcaaa gaaaccgacg tggtattaca accactgctg
    14341 catgactccc cggcagagct ttcacagccg tgtgaagtgc tcgactggcg caaaggcgag
    14401 tgcgatctga ttccaggcaa aaccgcgccg aatattgtgg cggtggagcg cgactaccct
    14461 gctacctatg aacgctttac ctcgctcggg ccattgatgg acaaacttgg caacggaggt
    14521 aaagggattt cgtggaatac ccaggatgaa attgatttcc tcggtaaact caattacacc
    14581 aagcgtgatg gcccggcaca ggggcgtccg ctgattgata ccgcgattga tgcctcagaa
    14641 gtgattctgg cgctggcacc ggaaaccaac ggccatgttg cagtcaaagc gtggcaagcg
    14701 ctgggcgaga tcaccggacg cgaacatacc catctggcgc tgcacaaaga ggacgagaag
    14761 attcgctttc gcgatattca ggcgcagccg cgtaaaatta tctccagccc cacatggtct
    14821 ggtctggaaa gcgatcacgt ctcctataat gcgggataca ccaacgttca tgagctaatt
    14881 ccgtggcgca cactgtcggg ccgtcaacag ctttatcagg atcatccgtg gatgcgtgcc
    14941 tttggtgaaa gcctggtggc ttatcgcccg cctatcgaca cccgcagtgt cagtgaaatg
    15001 cgccaaatcc cgccaaacgg cttcccggaa aaagccctta acttcctgac accgcaccag
    15061 aaatggggca ttcactcaac ctacagtgaa aacctgctaa tgctgacgct ctctcgcggt
    15121 ggaccgattg tctggatcag cgaaaccgat gcccgtgaac tgaccattgt cgataacgac
    15181 tgggtggaag tgtttaacgc caatggcgcg ctgacggccc gcgcggtggt cagccagcgt
    15241 gtaccgccgg gcatgaccat gatgtatcac gctcaggaac gcattatgaa tattcctggt
    15301 tcggaagtaa ccggcatgcg cggcggcatt cataactcgg tcacccgcgt ttgtccgaaa
    15361 ccaacacata tgattggcgg ttacgcgcag ctggcctggg gctttaacta ctacggcact
    15421 gtcggctcta accgcgacga attcatcatg atccgcaaga tgaagaacgt taactggctg
    15481 gatgatgaag gtcgcgatca ggttcaggag gcgaaaaaat gaaaatacgt tcgcaagtcg
    15541 gcatggtgct taacctcgat aaatgtatcg gctgccatac ctgttcggtg acctgtaaaa
    15601 acgtctggac cggtcgcgaa ggcatggagt acgcatggtt taacaacgtc gaaaccaaac
    15661 cgggcattgg ttatccgaaa aactgggaag atcaggatga gtggcaaggt ggctgggtgc
    15721 gtgatgtgaa tggcaagata cgcccgcgtc tgggcagcaa gatgggcgta ataaccaaaa
    15781 ttttcgccaa cccggtagtg ccgcagattg acgattacta cgaacctttc accttcgact
    15841 acgaacattt gcatagcgcg ccggaaggca aacatattcc caccgcccgt ccgcgttcac
    15901 tgattgacgg caaacggatg gacaaagtga tctggggccc aaactgggaa gaactgctgg
    15961 gcggcgaatt tgaaaaacga gcccgcgacc gcaacttcga ggccatgcaa aaggagatgt
    16021 acgggcagtt tgaaaacacc ttcatgatgt acctgccgcg cctgtgcgaa cactgcctca
    16081 atcccagctg cgtggcgacc tgccccagcg gcgctatcta caaacgcgaa gaagacggca
    16141 ttgtgctgat tgatcaggat aaatgccgtg gctggcgttt gtgcattagc ggttgtccgt
    16201 acaaaaaaat ctacttcaac tggaaaagcg gcaagtcaga aaaatgtatc ttctgttatc
    16261 cgcgaattga gtccggtcag ccaacagtct gctccgaaac ctgcgtgggt cgcatccggt
    16321 atctgggcgt gctgctctac gacgccgacc gcatcgaaga agcagcgagt accgaacgag
    16381 aggtcgatct ctatgaacgc cagtgcgaag tgttcctcga tccgcacgat ccctcagtga
    16441 tcgaagaagc cctgaaacaa ggtattccac aaaacgtgat tgacgctgcc cagcgttcgc
    16501 ctgtctacaa aatggcgatg gactggaaac tggcgctacc gctgcaccct gaataccgca
    16561 ccctgccgat ggtctggtac gttcctccgc tgtcaccgat tcagtcctac gctgatgcgg
    16621 gaggtttgcc gaaaagcgaa ggcgtgctgc ccgccatcga aagcctgcgt attccggtgc
    16681 aatatctcgc caatatgttg agtgccggtg ataccggtcc ggtactgcgg gcgctgaaac
    16741 ggatgatggc gatgcgccac tatatgcgtt cacaaaccgt ggaaggcgtt actgatactc
    16801 gtgccatcga cgaagtaggt ctgagcgtcg cccaggtcga agagatgtat cgttacctcg
    16861 ccattgccaa ctatgaagat cgtttcgtca tcccgacaag ccatcgggaa atggcgggcg
    16921 atgccttcgc agaacgcaac ggctgcggtt ttacctttgg cgacggttgc cacggttcgg
    16981 acagcaaatt caacctgttc aacagtagcc gtatcgatgc catcaacatc accgaagtgc
    17041 gcgacaaagc ggagggcgaa taatgcagat cctcaaagtg ctcggcctgt tgatggagta
    17101 cccagacgag ttgttgtggg agtgtaagga cgacgcgctg gcgcttatcc gccgcgacgc
    17161 gccgatgctc accgattttg cactcgacct gctcaacgcc ccgctgctgg ataaacaggc
    17221 cgaatggtgt gaagtgtttg accgcgggcg caccacgtcg ctgctgctgt ttgaacatgt
    17281 tcatgccgag tcccgcgatc gcggccaggc aatggtggac ctgctggcgg agtatgaaaa
    17341 ggtcggtctg caactgaatt gtagggaact gcccgattat ctaccgctgt atctggagta
    17401 tttaagcgta cttccggacg atcaggcgaa agaaggatta ctcaacgttg cgccgatcct
    17461 caccctgctt ggcggacgct taaaacaacg cgaggcaccg tggtacgcgt tatttgacgc
    17521 cctgttgcaa ctggcaggaa gccctctgtc aagtgacagt gtcacaaaac agattcgcag
    17581 tgaagagcgt gacgacaccc gccaggcgct ggatgcggtg tgggaagaag aacaggtgaa
    17641 gtttattgaa gataacgcca cggcgtgtga cagctcgccg cttaatcaat atcagcgacg
    17701 ctttagccag gatgtcgcgc cgcagtatgt cgacatcagt gcgggaggtg ggaaatgatt
    17761 cagtatctga acgtcttttt ttacgatatc tacccgtact tgtgtggcac ggtttttatc
    17821 cttggaagct ggctgcgcta cgactacggg cagtacacct ggcgcgccgc ctccagccag
    17881 atgctggata aacgcgggat ggtgctgtgg tcaaacttgt tccatatcgg cattttgggg
    17941 attttcttcg ggcacctgtt cggcatgtta acgccacact ggatgtacgc gtggtttctg
    18001 ccggtggcgg taaaacagca gatggcgatg atcctcggcg gaatatgcgg ggtattgacg
    18061 ctgattggcg gtgcggggct actggtgcgt cgtttaacaa atccgcgtat cagggcgacg
    18121 tcttctggtg cggatatcct gatcctcgcc attttgttaa ttcagtgcat tctcgggtta
    18181 accaccattc ctttttcagc acaacatccg gacggcagtg aaatgatgaa gctggtgggc
    18241 tgggcgcaaa gcattgtcac tttccagggc ggtgcatcta cgcatcttga tggtgtcgcc
    18301 tttatattcc gcgttcatct ggtgcttgga atgaccatct tcctgctctt cccgttcacc
    18361 cgactggtgc atgtctggag cgcgccgttt gagtacttta ctcgtcggta tcagattgtg
    18421 cgttcgcggc gttaattctc acgcgggccg gatagccctt cgctctccgg ccacttgcag
    18481 gctatgatag ttctccagcc acaggagaac gttaatgaaa ccgcaagtgt accacgtcga
    18541 tgcttttacc tctcaaccgt ttcgcggcaa ttctgccggt gttgttttcc ccgccgataa
    18601 tctcagtgaa gcgcaaatgc agcttatcgc ccgcgagtta ggtcattcgg aaactgcttt
    18661 tctgctgcac agcgacgaca gcgatgtgcg catccgctac tttacgccaa cggttgaagt
    18721 gccaatttgc ggtcacgcga cggtggctgc gcactatgta cgttcaaagg tattaggttt
    18781 gggaaattgc acggtctggc aaacgtcgct ggcaggaaaa catcgtgtga ctatcgaaaa
    18841 gcaaaatgat gattatcgta tttcgctgga acaaggtacg ccaggctttg agccaccact
    18901 gacaggtgaa acacgtgcag cgattatcaa cgcgcttcat ctcactgagg acgatattct
    18961 gccaggcttg ccaattcagg tggcaacaac aggacactca aaagtgatga tcccgttgaa
    19021 accagaagtg gatatcgacg ccctttcgcc tgatcttgct gcgttgaccg ctatcagcaa
    19081 acagattggc tgcaatggtt tcttcccgtt ccagatccgc ccaggcaaaa gcgaaaccga
    19141 cggtcgcatg ttctcgcctg cgattggtat tgtggaagat ccggtgaccg ggaatgccaa
    19201 tggtccgatg ggcgcatggt tagtacatca caacgtattg ccccacgatg gcaacgtgtt
    19261 gcgtgttaaa ggccatcagg ggcgcgcatt ggggcgtgat ggtgtaattg atgtgacggt
    19321 gacaattcgc gataaccaac cggagaaagt taccatttcc ggcacggcgg tgattctatt
    19381 ccatgctgaa tgggccattg atttttgatg ccgttgcctg atgcgcttcg cttatcaggc
    19441 ctacaaccgg actaatgtag gccggataag gcatttacgc cacatccggc aacctgacat
    19501 aaattatttt cccgcctccg ggtgcgtatc aaacgccgcc atcaccagcg ctaactcatc
    19561 cacagtaaag ccatgtttcg catcatctac gcccagacca aactgttcct gcatcacggc
    19621 atataacgac gccacatctg gtagatttcg ctgctcaact gcgtgcccat tttcataatg
    19681 ggtaaaatga aaattggtca gcgttagctt gccaccgtcc ggcaaatggc gacacatcag
    19741 caaatgatgg cgaaaatgcg actgcggcca gtgcgccgac cagaaattgc ctatcacata
    19801 atcgctttgt tgctgctcgc agagatcaaa acggtacatc gactgccagt gctgatgatg
    19861 attaaactgc aatacccaat catcaccctc ttgcaacaac cgatactctc cgtgtggcgt
    19921 ggtttgcatg atatccggca ttaaacgaat cggcgcggtt agcgtctgcc caccgaaacc
    19981 gacatcagca atccattttt ccccttccag ttccaccaac agcaaacgat gggtgcgcgg
    20041 cggtaatgct ggcggatttg ataacactac gcgacctaac aagctgcgaa cgttaaaccc
    20101 cagctcgcgt aacacccgct caaacacgcc attctgctca aaacagtaac cgccacgacg
    20161 ggctatcacc agtttctctt ccagcgattg atcatcaagc tgtatttccc tcggcagcaa
    20221 aacgtcgaga ttttcaaacg gaatggtgca attgtgtttc aggtgcaatg cacgcagcgt
    20281 atcaatattg gccgcagcag ctcccaacca gttaatacgg gcaaaataat gattcagaat
    20341 gggcgtcatc aggcgtttcc cttagtggtt ttctcaactt ataacctgga atagacgccg
    20401 cactggctat tttgtgcgat cgcggacatg catcacgttc gcaaatcgcc aatcccgtta
    20461 atttcgttac ttattgcgtt acgagctacg cttttaaaac acccttcata gcgaggcgat
    20521 atcgtgagcc aattcattcc cgtcgaatta caccatgcca gctgtctgtt aaatcatggc
    20581 ccaactgtca tgatcaccag tttcgatgag caatcccagc ggcgtaacat tatggcagca
    20641 gcctggtcga tgcccgtaga gtttgaaccg ccacgcgtgg cgattgtggt agataaatcg
    20701 acatggacca gggagttgat tgaaggtaac ggtaaatttg gcatcgttat cccgggcgtt
    20761 gcggcgacta actggacgtg ggcggtggga agtgtgtcgg gtcgtgaaga agataagttt
    20821 aattgctacg gcattccggt agtgagaggc ccggtgcttg gtttgccgct ggtcgaggaa
    20881 aaatgtctgg cgtggatgga gtgtcgcctg ctacctgcga cttccgcgca agaaaaatac
    20941 gatacgctat ttggcgaagt ggtatctgca gcggcagacg cacgagtatt tgtcgaaggc
    21001 cgctggcagt ttgatgatga taagctcaat acgttgcatc atttaggtgc tggcatgttt
    21061 gttaccagtg gtaagcgagt tacggcaggt taagcattac atgctgtaac cgggtttctt
    21121 tatcaaagcc tccatttggg gcgcgatttc ggccagggca agattacgaa agcccgctcc
    21181 ccgcaaggac tgacgcgaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta
    21241 accctgcctt gaatacctta tcattcgtta aaatattaat agcgatgtgc cgtatccctg
    21301 aaaataattc tgcggcgttt cctcttctta ttttgcagtc gtcttcattc attaccacgt
    21361 ccagacgcca gtgcagctta ttctccacgt gccagtggtt tcggattgct gtggcgaact
    21421 tctctgcggt taaatcagca gaactgatat aatatctgac cgtcatttct ggctctttct
    21481 tttgttctgc tattatggac cgaaaggaga ctgccacgca taatttcttc agtcctttcc
    21541 attcgaacgt gaaatcaata agttcatcag ggacatcgca aacaatatga agacggattt
    21601 cttctctgcc gtgactcttt tcactaatgg cgtaactgtc atgttctgga ttatttaatt
    21661 ctttcagtgg aaatttttcc tcaaaggctt tattaagccg cccctgattt ccttttacag
    21721 cgaataaata atcaccgccc tgtttttgta tcttctctgc aatatctttc tggcaaccca
    21781 tcgcatcagt tgtgatgatt tttcctttaa tatccagcat gttaagaagt tcagggatag
    21841 gtgtaatttc attggatttc tcatccgtcc tgatctgtcc gatgaccaga ctgtgcattg
    21901 ttgagaacgc actaatgaca tgaatcgctc ccttgcggcg actcttgtca taagaatgcc
    21961 ggagtgtttt tccatcaatt gcgatgacgt ctttatcatc tgaagaatgg cagtcacgca
    22021 tccagttaat aaagcactcg tgaaattttg caggactgat acaggataca actctggcaa
    22081 tggtatcgtg aacaggaata ccattatcaa aatcaccata ttgcttcaaa aaatcgagat
    22141 gtgtttcccc aaaatcctct atatcttccc agccttctg
//
LOCUS       DACADF010000076        21572 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.077, whole genome shotgun sequence.
ACCESSION   DACADF010000076 DACADF010000000
VERSION     DACADF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.077"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            9..821
                     /locus_tag="ISB30_002752"
     CDS             9..821
                     /locus_tag="ISB30_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAP1990366.1"
                     /translation="MVPQLQQQVTDLTSSLNSQEKKELTRKLESIFNNTQVQIAVLII
                     PTTKDETIEQYAARVFDNWRLGDAKRNDGILIIVAWSDRTVRIQVGYGLEEKVTDALA
                     GDIIRSNMIPAFKQQQLAKGLELAIIALNNRLTSQHQYSVNPSESESASSSDHYYFAI
                     FWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDLFLFSDKTFSIAVFFFFFTFT
                     TIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSGGGGSSGGGGASGRW"
     gene            complement(881..2179)
                     /gene="eno"
                     /locus_tag="ISB30_002753"
     CDS             complement(881..2179)
                     /gene="eno"
                     /locus_tag="ISB30_002753"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP1990367.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2267..3904)
                     /gene="pyrG"
                     /locus_tag="ISB30_002754"
     CDS             complement(2267..3904)
                     /gene="pyrG"
                     /locus_tag="ISB30_002754"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAP1990368.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4132..4923)
                     /gene="mazG"
                     /locus_tag="ISB30_002755"
     CDS             complement(4132..4923)
                     /gene="mazG"
                     /locus_tag="ISB30_002755"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAP1990369.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQIAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5019..7253)
                     /locus_tag="ISB30_002756"
     CDS             complement(5019..7253)
                     /locus_tag="ISB30_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP1990370.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7301..8602)
                     /gene="rlmD"
                     /locus_tag="ISB30_002757"
     CDS             complement(7301..8602)
                     /gene="rlmD"
                     /locus_tag="ISB30_002757"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP1990371.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8659..11415
                     /gene="barA"
                     /locus_tag="ISB30_002758"
     CDS             8659..11415
                     /gene="barA"
                     /locus_tag="ISB30_002758"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAP1990372.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFYHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11646..12986)
                     /gene="gudD"
                     /locus_tag="ISB30_002759"
     CDS             complement(11646..12986)
                     /gene="gudD"
                     /locus_tag="ISB30_002759"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAP1990373.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDQCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13007..14347)
                     /locus_tag="ISB30_002760"
     CDS             complement(13007..14347)
                     /locus_tag="ISB30_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAP1990374.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVGAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14349..15701)
                     /locus_tag="ISB30_002761"
     CDS             complement(14349..15701)
                     /locus_tag="ISB30_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1990375.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16135..16584)
                     /locus_tag="ISB30_002762"
     CDS             complement(16135..16584)
                     /locus_tag="ISB30_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP1990376.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(16602..17384)
                     /gene="truC"
                     /locus_tag="ISB30_002763"
     CDS             complement(16602..17384)
                     /gene="truC"
                     /locus_tag="ISB30_002763"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAP1990377.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLA
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17384..17713)
                     /locus_tag="ISB30_002764"
     CDS             complement(17384..17713)
                     /locus_tag="ISB30_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAP1990378.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18335..18880)
                     /gene="syd"
                     /locus_tag="ISB30_002765"
     CDS             complement(18335..18880)
                     /gene="syd"
                     /locus_tag="ISB30_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAP1990379.1"
                     /translation="MDDLTARALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18948..19796
                     /gene="queF"
                     /locus_tag="ISB30_002766"
     CDS             18948..19796
                     /gene="queF"
                     /locus_tag="ISB30_002766"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAP1990380.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQLAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19908..21272
                     /gene="ppnN"
                     /locus_tag="ISB30_002767"
     CDS             19908..21272
                     /gene="ppnN"
                     /locus_tag="ISB30_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAP1990381.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5305 a         5753 c         5142 g         5372 t
ORIGIN      
        1 aaccaactat tgtcccacaa ttacagcaac aagttacgga tcttacgagt agcttaaact
       61 cacaagaaaa gaaagaactg acgcgcaagc tagaatctat tttcaataat acccaagtac
      121 aaattgctgt attaattatt cccacaacca aagacgagac aattgaacaa tatgctgcaa
      181 gggtttttga caattggcgt ttaggagatg ccaaacgcaa tgatgggata ctgatcattg
      241 ttgcctggtc ggatcgcact gtccgcatcc aggtaggtta tggactggaa gaaaaggtaa
      301 ccgatgctct ggctggagat atcatccgta gcaacatgat acccgcattt aaacaacaac
      361 aattagctaa aggattagag ttagctataa tcgctttgaa taaccgactc acttcacaac
      421 accaatattc ggttaatcct tcagaaagtg aatcagcatc ttccagcgat cattactact
      481 ttgctatttt ttgggtattt gcagtgatgt tcttcccttt ctggtttttt catcaaggca
      541 gtaatttttg tcgcgcatgt aaaagcagtg tctgtatttc agcaatctat cttttagatt
      601 tattcctgtt ctccgataaa actttttcca ttgctgtatt tttcttcttt tttactttca
      661 ccaccattat ggtctttacc tgtttatgtg tacttcagaa aaaagcatct ggtagaagtt
      721 atcattcaga caacagcggt tccggtggcg gctcaagtgg cggcggattt agcggcgggg
      781 gcggttcttc tggcggtggc ggcgcgtccg gtcgttggta actcaaccat aaaaaacgcc
      841 agcccgaagg ctggcatttt taaatcagat aaagtcagac ttatgcctgg cctttgatct
      901 ctttacgacc gttgtacggt gctttttcgc ccagagcttc ttcgatacga atcagctggt
      961 tgtatttagc aacacggtca gaacggctca tagaacccgt tttgatctgg cctgcagcag
     1021 taccaacagc caggtcagcg atggtagcgt cttcagtttc gccagaacgg tgagagataa
     1081 ctgcagtgta gccagcgtct ttcgccatct tgattgcagc cagagtttcg gtcagagaac
     1141 cgatctggtt gaatttgatc aggatggagt tagcgatacc tttttcgata ccttctttca
     1201 ggatcttggt gttggttacg aacaggtcgt caccaaccag ctggattttg tcgcccagaa
     1261 ctttggtttg gtatgcgaaa ccgtcccagt cagattcgtc cagaccgtct tcgatggaaa
     1321 cgatcggata ctgtttggtc agttcttcca ggaagtgagt gaattcttca gaggtgaatg
     1381 ctttgttgcc ttcgccagcc agaacgtatt taccgtcttt gtagaattca gaagctgcgc
     1441 agtccatcgc cagagtgatg tctttgccca gttcataacc agcagcttta acagcttcag
     1501 cgataacagc cagagcttca gcgttggaac ccaggttcgg cgcatagcca ccttcgtcac
     1561 caacagcagt gttcatgcct ttcgctttca gaacttttgc caggtgatgg aaaacttcag
     1621 aacccatgcg gatggcttct ttcacagttt tcgcgccaac cggctgaatc atgaattcct
     1681 ggatatcaac gttgttgtca gcgtgctcac caccgttgat gatgttcatc atcggaaccg
     1741 gcatagagta tttgcccgga gtgccgttca gttcagcgat gtgctcgtac agcggcatac
     1801 ctttagcagc tgcagcagct ttggcgttag ccagagatac agccaggatt gcgtttgcgc
     1861 cgaatttgga tttgttttca gtgccgtcca ggtcgatcat gatcttgtca atgccagcct
     1921 gatctttagc atctttgcca atcagcgcct gagcgatcgg gccgttaacc gcagcaacag
     1981 ctttggttac gcctttgccc aggaaacggg atttgtcgcc atcgcgcagt tccagagctt
     2041 cacgggaacc agtagaagca cctgacggag cagctgccat accgacgaaa ccaccctcca
     2101 gatgtacttc ggcttcaaca gtcgggttac cacgggagtc gatgatttca cgaccgatga
     2161 tttttacgat tttggacatt aggttttcct caagtcacta gttaaactga aactccagac
     2221 aaacaacgcg tacccagggt acgcgttgcc gttctaactt ttttacttac ttcgcctgac
     2281 gtttctggaa ctcgctggcg gcttttacaa agccagcaaa cagcgggtga ccatcacgtg
     2341 gagtagaagt aaactccgga tggaactggc aagccacgaa ccacggatga ttcggaactt
     2401 cgatgatctc gaccaactga tcatccccgg aacggcccgc aacgcgcaga cctgcatctt
     2461 caatctgttt caacagcatg ttgttgactt cgtaacggtg acgatgacgc tcaacaattg
     2521 tcggcgcatt gtacagctgg cgaaccaggc tatcgtcaac caactggcac tgctgtgcgc
     2581 cgagacgcat ggtaccgccg agatcgctct tctcgctacg aacttcaacg ttgccgtttt
     2641 catcgcgcca ctcggtaatc agcgccacaa ccgggtactt acagtctggc acaaattccg
     2701 tagagttggc gttctccatg ttggcaacat ggcgagcgta atcaattaac gccacctgca
     2761 tacccaggca aatgcccaga taaggaatat tgttctcacg cgcaaaacgc gcggtcgtaa
     2821 tcatgccttc tacgccacga tagccgaaac cgccaggtac gaggattgcg tccagacctt
     2881 tgaggatttc aacgccgcgc gtttcaacat cttgtgaatc gatcagtttg atgttgacgc
     2941 tgacacgatt cttcagccca ccgtgtttca gtgcttcgat cacggatttg tatgcatccg
     3001 gcagctcaat gtatttgccg accataccaa tggtgacttc acttaccgga ttcgcttctt
     3061 cgaagataac ctgttcccat tcggacagat tcgcttccgg gcagtttaag ctgaatcgtt
     3121 tacaaatata atcgtccagc ccctgagatt tcaacaggcc cggaatttta tagatggaat
     3181 cgacgtcttt cagagaaata accgcttttt ccggaacatt acagaacaat gcaatcttcg
     3241 cacgttcgtt cgctggaacg gcgcgatctg aacgacaaat caggatatca ggctggatac
     3301 cgattgaaag cagctctttt acagagtgct gagtcggttt ggttttgact tcacctgacg
     3361 ctgccatgta cggcaccagc gtcaggtgca taaacagggt gtgctcacgg ccaatttcaa
     3421 cagccatctg gcgaatcgct tcgaggaacg gcagggattc gatatcaccg acagtaccgc
     3481 cgatttcgac cagtactacg tcatgacctt cgccgccttc cagcacgcgc tctttgatag
     3541 cgttagtgat gtgcggaata acctgtacgg ttgcgccgag atagtcaccg cggcgttctt
     3601 tacgcagaac gtcagagtag atacgacccg tggtgaagtt gttgcggcgg ctcattttgg
     3661 tacgaatgaa acgctcgtag tgccccaggt ccaggtcggt ttcagcgccg tcttcagtaa
     3721 cgaacacttc cccgtgttgg attgggctca tagtaccggg atcgacgttg atgtacggat
     3781 ccagtttcat gatggtcaca ttgaggccac gggcttcaag aatggctgcg agggaggctg
     3841 cggcaatgcc tttacccaga gaggatacga ccccgccggt cacaaaaata tagttcgttg
     3901 tcatgctgaa cctgagaagt taggttgaaa agacgatgga ataaccagga cgggaaagca
     3961 gtatacccga acatgacctg tgccacaaac tttcattatc cctcctcttc gccagcgcac
     4021 tattgaaatc aggagtgaga aaatagcccc tttggggtaa atgtttttga cgcaaatcaa
     4081 gcgcttgtca tttaaaaaat gacacaaatc gcgcttgacc acgtaattcc cttagagatc
     4141 aatttcctgc cgttttacct gttgccagac ttcttccatt gtttcgaggt caacacctgt
     4201 catttccagt ccacgcgcgg caacaatacg ctccacttcg cgaaaacgac gctcgaattt
     4261 ttcgttcgct ttttgcaatg cgatttccgc ttttgtccct aaatgacgag ccagattgac
     4321 cgtggcaaac agcaggtcac ccatttcctc ctccagttta gcctggtcga caacagcctg
     4381 ccgcgcctcg tacatcacct cgtcgatctc ttcgtagact ttatcgacta ccggaccaag
     4441 cgtcgtccag tcgaaaccaa cgttggcgca acgtttctgg attttttgcg cacgcattaa
     4501 agccggtaaa ctacgaggaa tatcgtccag cgccgaatgc tgcgctttct gcgcgcgctc
     4561 ttcggttttg atttgctccc aacgggcaag cacttcacta ctattttcgg cagaactgtc
     4621 agcaaaaaca tgcggatggc gacgctctaa tttatcgcta atagcagcgc aaatatcatt
     4681 aaagtcaaag cgcccttctt cctgagctat ttgtgcgtaa aacaccacct ggaacagcag
     4741 atcgcccagt tcaccgcgca gatcgtcaaa atcttcacgg gcgatggcgt ccagcacctc
     4801 gtaggtttct tcaagggtgt aaggcgcaat ggtggcaaat gtctgctctt tatcccacgg
     4861 gcagccgttt tccgggtcgc gcaggcgctg catgatggtg agcaaacggt cgatttgatt
     4921 cattgaatta tcctgaaaat tgcgggtccg tcaggtgtaa taccttcgag taaaagatat
     4981 cgctggatac ggctgacgcc atatccagct atgtaagatt aactcccgtg caaccgacgc
     5041 gcgtcgataa catccggtac ctggttgagt ttacccagca cgcgacccag cacttgcagg
     5101 ttgtaaatct caatggtcat gtcgatggtc gccagttgct gtttggtgtc gctacggctg
     5161 gcaacgccaa gtacgttcac cttctcgttg gcgagaatgg tggtgatatc acgtaacaac
     5221 ccactacgat cattagccac cacgcggacc accagcgaat atccggcgga gtagctctcg
     5281 ccccataccg cgtcaacaat gcgttctggc gcatgggagc gcagttccgc caattgttcg
     5341 caatcggcgc ggtgtactga aataccgcgc ccctgggtaa tgaagccgac aatctcatct
     5401 ccaggaatcg gctggcagca gcgcgcgatg tggtgcatca ggttaccgac cccttctacc
     5461 accacacgac cgttatcttt actgcggttt tgcggcgtgt agcttttttg ctgaagctgt
     5521 ttcagcgcgg cggcgtcctg ctcttcggca ctcggcttat taaattgcga ttgcaggaag
     5581 ttcaccatct gattgagacg gatatccccg ccaccaatcg ccgccagcaa ctcgtcgaca
     5641 tcattgaagt tgtaacgcgg cagcagatgt ttttctgctt ctttcaggct gatccccaga
     5701 tgttccagct cgtcgtcaag gatttgccgc ccagccagaa tgtttttgtc acggtcctgt
     5761 ttacggaacc aggcgtgaat tttcgaacgc ccacggctgg ttgtgacgta accgaggttt
     5821 gggtttaacc agtcacggct ggggttcggc tgtttctggg tgataatttc aatctggtcg
     5881 cccatctgca gctggtaggt gaacggcaca atgcgcccgc caatttttgc cccgatgcag
     5941 cggtgtccga catcactgtg gatgtggtaa gcgaagtcca gcggcgttga tcccgcaggc
     6001 aaatcaacga catcaccttt cggcgtaaag acgtacactc ggtcgtcaaa gacctgacta
     6061 cgtacttcgt cgagcatttc gccggaatca gccatctctt cctgccacgc aatcagttta
     6121 cgcagccagg caatccggtc ttcatgtccc gaacgtgcgc cgccagcagc cgcgccctct
     6181 ttatatttcc agtgcgcagc aacacccaac tctgcatctt catgcatctg tttggtgcgg
     6241 atttggatct caacggtttt gccacccggc cccagaacca cggtatgaat agactgataa
     6301 ccgtttggtt tcgggttagc gacgtaatcg tcaaactcat ccggcaggtg gcgatagtga
     6361 gtgtgcacta tccccagtgc ggcataacaa tcctgtaaac gctcggcgac aatacgtacc
     6421 gcacgcacat caaacagctc atcgaaggcg aggttctttt tctgcatttt gcgccagatg
     6481 ctgtagatgt gtttcggacg gccatacact tcggctttaa cgccttcagc tttcatctca
     6541 gcgcgcagat ggccgacaaa ctcttcgata tagtgttcgc ggtcgagacg gcgttcatgc
     6601 aacagttttg cgatgcgttt gtattcggtc gggtggagat aacggaagca gtaatcttcc
     6661 agctcccatt tcagttgccc aatcccaaga cggttcgcca acggcgcgta gatattggtg
     6721 cactcttttg ccgccagtac acgttcatct tccggcgcat cttttacttc gcgcagatga
     6781 gcaatacgct ccgccagttt gatgactacg cagcgaaaat catcgaccat cgccaataac
     6841 atccggcgaa cgttatcgac ctgttcggag gaaacagaat cagtgtgcgt cgctttcagc
     6901 tggcggatcg ccgccatatc acgcacgccg tgaataaggt taacgaccga cttaccgacg
     6961 ctctcgcgca gcacatcttc gctgactacg ttggcatcag ccagagggaa tagcagcgcc
     7021 gcccgcagcg tgtcaatgtc catacttaat gtcgagagga tctccaccat ctcaacacca
     7081 cgccacaata acagactggc atccggatgc ccctgcgtct gttgcagaca atacgcccag
     7141 gtttcggcta agcactcaca cgacttctgg ctggtaatac ccagacttgc gatccatttt
     7201 tccggatcaa attcaccagc cttattgata tgtgcacttc ttaccgcaac catcgtcctc
     7261 tcctttaggg accagacctg ccgaaatcgg caaatcgtaa ctatttaacg cgcgagaaaa
     7321 gtaccatcga ttccagatgt cccgtgtgtg ggaacatatc cagcatcgcc agtcgcgcaa
     7381 tggtatatcc tgcttttaat aacgcttcgc tatcccgagc cagcgttgca gggttacagg
     7441 atacataaac tatacgaata ggttccagtt ttataatttg ctgcataaca cctgcggcac
     7501 ctgctcgcgc cgggtccaac aacactttat cgaagccgtt tttcgcccac ggctgctttg
     7561 tgacatcttc ttcaagattt tcgtgataaa acgtcacatt ctgtaagcca ttaagacgcg
     7621 cattctgctg gcctttttcc accagcgccg gaacaccttc tacaccgacc acactggcag
     7681 cttgtgtcgc caatggcagt gtaaagttgc ccataccgca gaacagatcc agtacgcgat
     7741 cttcaggttc cacatccagc cattccaacg cacgcgctac cattttttgg ttcacacccg
     7801 cattgacctg aataaaatcg cgcgggctaa aagttaagcg caacccattt gagtcatacc
     7861 agggcatctc accagagacg gtttcgagta tctcactatc gggggcgaga tacagatcca
     7921 ggccttcaga atgcgaaaag cattccagtt tttcgcgatc tgccgaactt agcggtgcgg
     7981 tatggcgcaa aatcattagc gtgccgctgg ttgcctgtac cagttcaaca tgaccaagat
     8041 ggcgcatagc ttgtaagctg cccaggcatg ccctgacttt gggcagcaat gcttctagtt
     8101 ggggcactaa aatggggcat tgtttaacgt cgacaatgtc actggagccc gctttgcgaa
     8161 accccatctg aagttgctgt gttttcggta agtagttcag acttaaacgc gcgcgacggc
     8221 gatagcccca aggaacatcg gcgatcactt cagagacttc gtgtttcatt aatcgggcga
     8281 gtgccgcact tttgcttcgc tgctgtaaat ccacgctggc gtgttgttgc tgacagccgc
     8341 cgcatacgcc aaaatgagga cagcgtggcg tttcgcgttc cgggctatcg cttaaccggc
     8401 gtacgacttt agcgcgggca tactgttttt tatcttcagt aacagtaact tccgcgtttt
     8461 cctgcggcaa taatccgggg ataaataatg ttttgccgtt atgtcgcgcc acaccctgac
     8521 caaaagagtc gaggtcgttg actgaaacgg ttatgatctg acgcgtcgtc gtgcgtcgtt
     8581 ttgcagagta gaattgcgcc attggcgaga ctttctcaat ttaacagtgt gaccttaatt
     8641 gtcccataac ggaactccat gaccaactac agcctgcgcg cacgcatgat gattctgatc
     8701 ctggcaccga ccgtccttat tggtttattg ctgagtatct ttttcgtcgt gcatcgctat
     8761 aacgacttgc agcgtcaact ggaagatgcc ggtgccagca ttattgagcc gcttgcagtt
     8821 tctactgaat atggcatgag tctgcaaaat cgcgaatcta tcggtcagtt aataagcgta
     8881 ctgcatcgtc gccattctga tattgttcgc gcgatttcgg tttatgatga aaataaccga
     8941 ctctttgtca cctccaattt tcatcttgat ccctcatcaa tgcagctcgg cagcaacgtg
     9001 ccgtttcctc gccagctcac tgtcactcgt gacggcgata ttatgatcct ccgcacgccg
     9061 attatttctg agagttactc ccccgacgaa tcgcccagta gcgatgccaa aaatagtcaa
     9121 aatatgttgg gatatattgc gcttgagctg gatcttaaat cggttcgctt gcagcaatat
     9181 aaagagatct ttatttccag cgtgatgatg ctgttttgta tcggtattgc gcttattttt
     9241 ggctggcgct taatgcgcga tgtaaccggt ccgattcgca acatggtgaa taccgtcgac
     9301 cgcatccgtc gcgggcaact cgacagccgg gtggaaggat ttatgctcgg cgagctggat
     9361 atgctgaaaa acggtatcaa ctcgatggca atgtcgctgg cggcttatca cgaagagatg
     9421 caacacaata tcgaccaggc gacgtccgat ctgcgtgaga cgctggagca aatggagatt
     9481 cagaacgttg agttggatct ggcgaaaaag cgcgcccagg aagcggcgcg tattaaatcc
     9541 gagtttctgg caaatatgtc acacgagctg cgtacaccac tgaatggtgt tattggcttt
     9601 acccgcctga cgctgaaaac agaattaaca ccaacgcagc gcgatcacct gaatacgatt
     9661 gaacgttcgg caaataattt gctggcaatt attaatgatg ttctcgactt ctcgaaactg
     9721 gaagccggta agctgattct ggaaagtatt ccgttcccac tgcgcagcac gctggatgaa
     9781 gtcgttaccc ttctggcaca ctcttcccac gataaagggc tggaattgac gctcaatatt
     9841 aagagcgacg tccccgataa cgtgattggc gacccgctac gtttacagca aatcatcact
     9901 aacctggtgg ggaacgcgat taaattcacc gagaatggca acatcgatat tctggtggaa
     9961 aaacgtgcgc tgagtaatac caaagtgcaa attgaagtgc agattcggga taccggcatt
    10021 ggtattcctg aacgcgatca atcgcgctta ttccaggcct tcagacaggc tgatgccagt
    10081 atttcccgcc gtcatggtgg caccgggctg gggctggtga ttacacaaaa actggttaat
    10141 gaaatgggcg gtgatatttc gttccatagc ctgccgaatc gtggttcaac tttctggttc
    10201 cacattaatc tcgatctgaa cccgaacatt attatcgaag ggccatccac ccagtgcctc
    10261 gctggtaagc gcctggccta tgtcgaacca aactccgcag cagcacaatg cacgctggat
    10321 attttaagtg aaacgccgct ggaagtggtt tatagcccaa cgttctccgc gctgcctccc
    10381 gcgcattacg acatgatgtt gttaggcatc gcggtgacct tccgcgagcc gctaacaatg
    10441 caacatgagc gattagcgaa agcggtatcg atgaccgatt tcctgatgct ggcacttcct
    10501 tgccatgcac aagtcaatgc tgaaaaactt aagcaagatg gtatcggcgc gtgtctgctg
    10561 aaaccattaa cacctacgcg cctgttgcct gccctgacgg aattttatca tcacaaacaa
    10621 aacacgcttt tgcctgtaac cgatgaaagt aagctggcaa tgacagtcat ggcggttgat
    10681 gacaaccccg ctaacctgaa acttatcggc gcattgctgg aagatatggt gcaacatgtg
    10741 gaactttgcg atagcgggca tcaggcagtt gaacgggcga aacagatgcc gttcgattta
    10801 atcttaatgg atattcaaat gccggacatg gacggcattc gggcatgtga gctcatccac
    10861 cagctcccgc atcagcagca aacgccggtt atcgcggtaa cggcgcatgc gatggccggg
    10921 caaaaagaga aactgcttgg cgcggggatg agtgattatc tcgccaaacc gatcgaagaa
    10981 gagcgattgc ataatttgct gttgcgctac aagcctggca gcggtatttc ctctcgcgtc
    11041 gtgacgcccg aagtcaacga aattgtggtc aacccgaatg cgaccctcga ctggcaactg
    11101 gcactacgcc aggcagcagg aaaaaccgat ttagcgcgcg atatgctgca aatgttactc
    11161 gatttcctgc ctgaagttcg caacaaagtt gaggaacagc tggttggaga aaacccggaa
    11221 ggcctggttg atttgattca taaactgcat ggcagttgcg gctatagcgg tgtaccgcgt
    11281 atgaagaatc tctgccaact gatcgaacaa cagctacgta gcggtactaa agaagaagat
    11341 ttggagccgg agctgctgga actgttggac gagatggata atgtagcgcg cgaagccagc
    11401 aaaattctcg ggtaatggac gtcttgtcga gtagcaaccg gatttttatg acgctggttt
    11461 cagattggtg acaagtgcct gtgtttatgc cggatgcggc gttaacgcct tatccggcct
    11521 gcaaaaccaa ttaaattcaa tgaattaaaa aatgatgtag ccctgatacg tagcgcaaca
    11581 ggcaattttg cgtttggcat cagtctcaaa ccggctccag atagagccgg ttttggtttt
    11641 ctgtcttaac gcaccatgca cggacgcttg ttatcgaacg tccagccggg gatcaggtac
    11701 tgcattccca tcgcatcgtc acgcgcgcca agcccgtgtt tctgatacag ctcgtgcgct
    11761 ttcatcactt gatccatatc gatttctaca cccagccccg gtttttctgg cacctgaacc
    11821 agaccgcctt tgatctctaa cggttctttg gtcaggcgct gattaccttc ctgccagatc
    11881 cagtgcgtat caatcgcggt gattttaccc ggtgcggcag cggcaacatg ggtaaacatc
    11941 gccagggaaa tatcgaagtg gttgttggag tgtgaacccc aggtcaggcc aaattcatga
    12001 cacatttgcg ccacacgtac cgaaccttgc atcgtccaga aatgcggatc cgcaagcggg
    12061 atatcaacgg attgcaacga gagcgtatgg cccatctgcc gccagtcggt ggcgatcata
    12121 ttggttgcgg tcggcaggcc agttgcgcga cggaactctg ccatcacttc acgcccggag
    12181 aaaccttgct ccgcaccaca cggatcttct gcataagcca gcgaaccttt caggtatgta
    12241 ccgattttaa tcgcttcgtt cagcgaccag gcaccgttag gatcgagcgt aatacgcgcc
    12301 tgcgggaaac gttgcgccag tgccacaata gattctgctt cttcttcccc ggccagtaca
    12361 ccgcccttca gtttgaaatc gttgaagcca tatttttcat atgccgcttc cgccaggcgc
    12421 accaccgcat ccggcgtcat cgcttcttcg tgacgcaggc gataccagtc acattgatca
    12481 tccggctggc tttgatacgg cagcggtgtg gctttgcgat taccgacgaa gaacagataa
    12541 ccgagcattt cgacttcgct acgctgttgg ccatcgccca gcagcgatgc cacgtttacc
    12601 cccagatgct gccccaacag atccagcatt gccgcttcta tcccggtaac tacatgaata
    12661 gtggtacgca aatcaaatgt ctgcaaaccg cgcccaccgg catcacgatc ggcaaacgta
    12721 ttacgcacca gcgtcagaac gtttttgtat tcacccagcg ttttacccac caccagcgga
    12781 atcgcatctt ccagcgtttt acggattttc tcgccgccgg gaatttcccc tacgccagtg
    12841 tgaccggaat tatctttgat aatcacaata ttacgcgtga agaacggtgc atgtgcacca
    12901 ctcagattca tcagcatact gtcatgaccc gccaccggga tgacctgcat ttcagtaaca
    12961 acaggcgtcg taaattgaga actcatattt atgtcctttt tcagaatcaa tgacggccga
    13021 aaacggggcg tttacggtca aaggtccagc cggggatcag gtactgcatc gggcctgcgt
    13081 cgttacgcgc accgccaggc agacgtttat aggcctcatg tgccttttgt acctgttccc
    13141 agtccagttc cacgcccaga ccgggcgcat caggaacggc aatttttccg tttttaatct
    13201 ccagcggatt tttggtcaga cgacaatcgc cctcctgcca aatccagtgg gtatcgatag
    13261 cggtaggatt acccggtgcc gccgcgccca catgggtaaa catcgccaga gagatatcaa
    13321 agtggttatt agaatggcag ccccaggtca gcccccagtc gtcgcaaagt tgcgccacgc
    13381 ggactgcacc ggaaagcgtc cagaagtgcg gatcggcaag tggaatatct accgcattga
    13441 gcatcaccgc atgacccatt tcgcgccagt tggtggcaat catgttggtc gcgacgggca
    13501 agccggtcgc ccgtcgaaac tccgccatca cttcacgacc ggagaaaccc tgctcagcgc
    13561 cgcacgggtc ttccgcatag gtaagaacat cattcagccc tttgcacaga gaaatggctt
    13621 catcaagcag ccaggcacca ttgggatcaa cggtaatccg cgcatccggg aagcgtttct
    13681 tcaatgcacg aacagtgtcg atttcttgct cgccaggtaa cacgccaccc ttaagtttga
    13741 aatctttaaa gccatagcga tcctgtgagg cttccgccag acgcacaacg gcttcgctgt
    13801 tcatcgcttt ctgatgccgc aactgatacc actcatggtt gcccggcgta ttttccagat
    13861 aaggaagatc ggttttggtc cgatcaccga tataaaacag ataaccgaga acggtaatag
    13921 catcacgttg cttgcctggc cctaacagtt cgcagaccgg aacattcagc gcctgaccta
    13981 acaggtcaag caaggcggct tccagcgccg ctacggcatt aacgcgcaat tcaaaagtcc
    14041 aggcaccttt gccgaaggta tcaaaatcgg ctgcctgatt acctttatgc acctgctgaa
    14101 ccactttatt caggcgcgca acttcctggc ccagcaccat cggaatagcc ccgaccagtg
    14161 tttgataaat cacctctccg cccggcgctt caccaatgcc ggtatgcccg gcattatcgg
    14221 tgagtaccac aatattgcgg gtgaaatatg cgttatgtgc accaccaata ttaagcaaca
    14281 tgctgtcatg tccggcgacc ggaatgactt tcatatcagt aataacagga ctggattgtg
    14341 tcgtcatctt attgccccgc gacaggtttc agctcaatac gcttgatatc gcccaccagc
    14401 accaggtagc tgagtaccgc gattaaggca tgaacaccaa cataaatcag cgccccatta
    14461 aaggagccag tcgtgccaac gatataacca attgcgattg gcgtgacgat gccagaaatg
    14521 ttaccaaaca tgttgaacag gccaccggaa agaccgctga tctcttttgg cgcagtatca
    14581 gccattacag cccaacccag cgcaccgatc cccttaccga agaaggccag cgccataaag
    14641 ccgatgatca tccactcaac gttgacgtag ttgcagaaca ccatcaccat cgacaacaac
    14701 atgcccatca cgatcggtgt tttacgcgca atgttcagtg aacccgtgcg acgcatcagc
    14761 cagtcggaaa taatcccccc caacacaccg ccgataaaac cgcaaaccgc cggaacggaa
    14821 gccacaaagc ccgctttcag aatcgacatc ccgcgcgcct gcaccagata aaccgggaac
    14881 caggtaatga aaaagtaagt cagggcgttg atacagtact gaccgatata aacgccgatc
    14941 atcatccgtg acccgagcag ctgtttgatc tgcccccact tcacgctgaa cggcacttta
    15001 actttggtgt tttgctgatc catattgatc agcgcgccac ccgcggcgat gtactccagc
    15061 tctttctgat ttacgcccgg atgttgattt ggctcgtgga tgactttcaa ccagataaag
    15121 ctgatgacaa tgcccagacc gcccataaag aagaagacgt gtgaccagcc cacttcatga
    15181 gtcagccagc ccataatcgg cgcgaagata accgttgcga agtattgggc ggagttaaaa
    15241 atcgacaccg ccgttcccct ttcctgcgcc ggaaaccagg ccgcaacaat gcggctgttg
    15301 ccagggaaag atggcgcttc agcaagcccg accaggaagc gcagtgtaaa cagggccacg
    15361 ataatgccga atccactaaa gatatcgacg aagccttgca gcaaggtaaa catcgaccag
    15421 ataaagatcg accagaagta gacgcgtttt gaaccaaaac gatccagcaa ccagccacca
    15481 gggatctgcc cgataacata agcccatgag aaggcagaga acacatagcc cattcccacg
    15541 ggatcaaggc cgatatcttt ggccatttcc gaaccggcaa tagaaagcgt agcgcggtcg
    15601 ccgtagttga aggatgtgac gataaacaac atcaccacta tccagtaacg agcattcgtg
    15661 cgtttttcca cactgctcgc agcctgactt aaagaactca ttgttgcact cctgaaaatt
    15721 cgcgttagcc acgctcactc tggactgcga catcgccagg aaatcagagg tgacgtaggg
    15781 tgtttttgcc gtttttatag gtcgttcgcc gaatacggcg cgtgtttata tctggcaata
    15841 gcagtataaa aagcgcgcca tagcggctca ccgtgcaaca acacaacatt aatgcgttca
    15901 atgaggcccg attttggcat tagccctgga cggtggaatc cacttcacgg aaatgaaaac
    15961 aagaacaaga aaggaagggt taaaacgaag aaataaagaa gagtatgaaa tggatcgctt
    16021 gactccaggc aaacgccagt aaaaatccgc gctatgaagc agtttttact ggcatttgcc
    16081 tgaaaagatt cgattcagca ccgctaaaac ggcatttacc gctcgctgaa catatcagga
    16141 caacatcgtg ccccaatgtt caacccacgg atttgattcc gtttccggtt ccgggttctc
    16201 actggcgtca atcaacagca tttcgccaac ccgctgagcg ctctgttcct gcaacaaggc
    16261 atcgaactgt ttgccgccat tgcagaaatt cacataacta ctgtcgccga gcgcaatcac
    16321 gccataacgc agattcggct ggaagcccag ggtatcttta attccctgaa agagcggcac
    16381 aatgctatca ggaaggtcgc cctgcccggt cgtggacgta accaccagaa catacttatc
    16441 ctgatagggc agccagtcgc ttaattcagg atcttcaaat accgttgctt tgtggccctg
    16501 cgcggtcaga atcgcttccg cttcttcggc cactaacagc gaatttccgt acatagtgcc
    16561 gacaaaaata ccaatttccg ccataccgtt ttctccctgg attaggaact tatctctcca
    16621 tcctgacccg atggcgcact gaactcaacc ctttcatttt caggaagcag accccgccag
    16681 ccaaattgtg ataacgcctg catccaggtg tcgtccaggc ccgcgtgaat agccagcggc
    16741 tcgccagtaa aaggatgcgt caatgacaac tgactggcat gcagcattaa ccgctgaagg
    16801 ccaaaatgct cagcaccgct gcgattctgg cgtaaatcac catgtttgct gtcgccaata
    16861 atcgggtgcc gcaaatgggc aagatggcga cgaagctggt gcttgcgtcc agttttcggc
    16921 tccagttcca ccaggccgta gcgcgtggtc gggtaacgtc cagtcgctac cggcatttct
    16981 acggtcgcca gaccgcgata atgcgtcact gctggctgcg ggcctttatc ttcgcgggca
    17041 aatttatcag cgattttgtc cagttcttcc accagtggat aatccagcac cgcttcttcc
    17101 atcaaccagc cacgcacaat cgcatggtaa cgtttctgga tttggtgctg ttcaaactgt
    17161 tgtgccagca gccgtccggc ttcgctggat aatcccatca acaacacacc agaagttggt
    17221 cggtccagac gatgagcagt aaaaacatgc tggcctatct ggtcacgcac ggtttgcatg
    17281 accactactt tctcgtcgcg atccagccag ctgcggtgaa ccagccagcc ggagggttta
    17341 tttaccgcaa ccagccattc atcctgatag agtatttcca gcattagctc gcatcatccg
    17401 caaaaagagc atccagtttc tccagctcag ccagaataag cgcgcgttgc ggatgatcag
    17461 tcgccagcgc catttcataa tagggcgcaa cagcaaaagc gcccggtaac ggctggttgt
    17521 tatctaacaa atcgtgcatt cgcgggatca gcacccactg caaccactcc agtggttcca
    17581 tggtgtccat aaagaacggt tgggtactat taaattgatg cggctggggt tcatcgtttc
    17641 gccagtgctg atgttcacgc agtaacgctt caagcgcctg caactggaga cgaacgcggt
    17701 catgagtggt cataaagcaa cctcaataag aaaaactgcc gcgaaggata gcaggaataa
    17761 aaaaagggag cactgtattc acagtgctcc cggttcgttt cgcagcattc cagctacttt
    17821 tgttgctccc tgctcatcct tgacaacttt tcctctggcc ttgcggccaa tcgttcatcc
    17881 tgaactattg cttcctgctc acaccacccc gatgtgatac ttcatcctga agtgtccctg
    17941 gccttcctga cccaccgaat catcctgacc ggttctcatt ctccatcctg gaggtgtcct
    18001 ttaacgcgtc ctgcgtcatc ctcttcgctt catccagaag cctttccctg aaacaccatc
    18061 ctggtgtgtc cttcagaagt gtcatcatcc tgatgttcac ttcgttgtct gactccctgt
    18121 cgacgaagat agaatcgtct ttttctcgaa gtcttacaag gcgcttacag acaatgcctt
    18181 aaggaaaatt tcatatgaaa gttaagaatt aacaagcaac atgttgtttt taaatgagaa
    18241 taacgattct tatcttttta agtattcgct tatttcctgg cgatctccta cagagtctgt
    18301 aagagatctc tcacaatcag atggggtatt tggattacag aagaggctta agttgattaa
    18361 ggaattccgc aagatttgag gctaaatgcg tccgtttacg tgttcccagc gtctctttgc
    18421 acacttcacc ggagagatta cacaccgaga tgacctctaa ctcttcttcc agcgtcgcga
    18481 taaatagcgt gggcggcagt tttagccttt tctgagtcac cagatgacca atcagatttt
    18541 cctgcacccg acggaagtcg tcttcactcc aggtttgcag caatgtcagt ttgatatcgc
    18601 caaactgcgc gtgcatatcc ccggcaaact gagtcgtata aaaggtatga atcgtgggtt
    18661 gtatcacaat atcaaaagcg cgttcaaccg cgtttacatt ttgttccccg gtaaacggct
    18721 ggggttgcca gtatacggca tcttcggtag ttgaaataat gcacggtgaa ggaacgccgt
    18781 acagttcctc acttaacggc cagcttttat gctcttcatg ccacgcatcg cagtagcgtg
    18841 cagtaaaatc tttcagggct cgtgcggtca aatcgtccac taatttctct cttcacttaa
    18901 accagataca cttgtcgttt agtttatctg gtttatgacg gtgaaacatg tcttcttatg
    18961 caaaccatca ggcacttgcg ggcctgacac ttggaaaatc aaccgattac cgggatacct
    19021 atgacgccag cctactgcaa ggcgttccac gcagcctgaa tcgcgacccg ctgggtctga
    19081 aagcggataa cctgcctttt cacggtacgg atatctggac gctgtatgaa ctttcctggc
    19141 tgaatgcgaa aggtttgccg caactcgctg tcggtcatgt tgaacttgat tacaccagcg
    19201 taaatctgat tgagtcgaag agttttaagc tctatctcaa cagttttaac cagacgcgtt
    19261 ttaataactg ggatgaggtg cgccagacgc tagagcgcga cttaagcact tgcgcccagg
    19321 gtaaggttag cgtggcgtta tatcgtcttg atgaactgga aggccagccg ataggtcatt
    19381 tcaatggcac ctgcattgat gaccaggata tcactatcga taactatgaa ttcactactg
    19441 actatctgga gaatgccacc agcggtgaaa aagtagtgga agagacgctt gtcagccacc
    19501 tgctgaaatc aaactgcctg atcacccatc aaccagattg gggttccatc caaattcagt
    19561 atcgtggacg ccaaattgac agagaaaaac tgctgcgtta cctggtctct ttccgtcatc
    19621 acaacgagtt ccacgaacag tgcgtggaac gcatctttaa tgacctgtta cgcttctgcc
    19681 agccagagaa attgagcgtt tacgcacgtt atacccgtcg tggcggtctg gacattaacc
    19741 cgtggcgcag taatagcgat tttgtcccat cgaccacaag actggttcgg caataaattt
    19801 tttctcaatt ttgcgtgctg gattcacgca gaaggttgtg aaaggtcatc aggcagggct
    19861 attgtaatca aagggaatga cgatattcgt cccataagga gtttttcttg attacacata
    19921 ttagcccgct tggctccatg gatatgttgt cgcagctgga agtggatatg cttaaacgca
    19981 ccgccagcag cgacctctat caactgtttc gcaactgttc acttgccgta ctgaactccg
    20041 gtagtttgac cgataacagc aaagaattgc tgtctcgttt tgaaaatttc gatattaacg
    20101 tcctgcgacg tgaacgcggc gtaaagctgg aactgataaa tcccccggaa gaggcttttg
    20161 tcgatgggcg aattattcgc gctttgcagg ccaacttgtt cgcggttctg cgtgacattc
    20221 tcttcgttta tgggcaaatt cataacaccg ttcgttttcc caacctgaat ctcgacaact
    20281 ccgtccacat cactaacctg gtcttttcca tcttgcgtaa cgctcgcgcg ctgcatgtcg
    20341 gtgaagcgcc aaatatggtg gtctgctggg gcggtcactc aattaacgaa aacgagtatt
    20401 tgtatgcccg tcgcgtcggg aatcagttgg gcctgcgtga actgaatatc tgcaccggct
    20461 gtggtccggg agcgatggaa gcgccgatga aaggtgctgc ggtcggacac gcgcagcagc
    20521 gttacaaaga cagtcgcttt attggtatga cagagccgtc gattatcgcc gctgaaccgc
    20581 ctaacccgct ggtcaacgaa ttgatcatca tgccagatat cgaaaaacgt ctggaagcgt
    20641 ttgtccgtat cgctcacggt atcattatct tccctggcgg tgtgggtaca gcagaagagt
    20701 tgctgtattt gctgggaatt ttaatgaacc cggccaacaa agatcaggtt ttaccattga
    20761 tcctcaccgg cccgaaagag agcgccgact acttccgcgt actggacgag tttgtcgtgc
    20821 atacgctggg tgaaaacgcg cgccgccatt accgcatcat cattgatgac gccgctgaag
    20881 tcgctcgtca gatgaaaaaa tcgatgccgc tggtgaaaga aaatcgccgc gacactggtg
    20941 acgcttacag ctttaactgg tcaatgcgca ttgcgccaga tttgcaaatg ccgtttgagc
    21001 cgtctcacga gaatatggct aatctgaagc tttacccgga tcaacccgtt gaagtgctgg
    21061 ctgccgacct gcgccgtgcg ttctccggta ttgtggcggg taatgtaaaa gaagtcggta
    21121 ttcgcgccat tgaagagttt ggtccttaca aaatcaacgg cgacaaagag attatgcgtc
    21181 gtatggacga cctgctacag ggttttgttg cccagcatcg tatgaagttg ccaggctcag
    21241 cctacatccc ttgctacgaa atctgcacgt aatctcccct cccccggtga cgtttgttgc
    21301 cgggttttat cgtttttggt cacttactca tcaactcatt tcatttgtta tatgaatgtt
    21361 tcttaccaac ctcacgcgac aaatatcacc acagttaata tgtcatacaa tttatgttga
    21421 aacgcaaacg tttccctatt ttcataaacc acattttatc gccatgaaaa agaaattatc
    21481 gccatgatta actaaaagta ttgatttttc cagttcaacc tacatatatt gcgcgcccca
    21541 gaagaagtca gatgtcgttt aatgggcaaa ta
//
LOCUS       DACADF010000077        21004 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.078, whole genome shotgun sequence.
ACCESSION   DACADF010000077 DACADF010000000
VERSION     DACADF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.078"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            121..1143
                     /locus_tag="ISB30_002768"
     CDS             121..1143
                     /locus_tag="ISB30_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAP1990382.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQHITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1143..2123
                     /locus_tag="ISB30_002769"
     CDS             1143..2123
                     /locus_tag="ISB30_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP1990383.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            2120..2878
                     /locus_tag="ISB30_002770"
     CDS             2120..2878
                     /locus_tag="ISB30_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1990384.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            2888..3700
                     /locus_tag="ISB30_002771"
     CDS             2888..3700
                     /locus_tag="ISB30_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP1990385.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            3697..4551
                     /locus_tag="ISB30_002772"
     CDS             3697..4551
                     /locus_tag="ISB30_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAP1990386.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CISGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAASI"
     gene            4577..6547
                     /locus_tag="ISB30_002773"
     CDS             4577..6547
                     /locus_tag="ISB30_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAP1990387.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(6597..6851)
                     /locus_tag="ISB30_002774"
     CDS             complement(6597..6851)
                     /locus_tag="ISB30_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP1990388.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7052..7786
                     /locus_tag="ISB30_002775"
     CDS             7052..7786
                     /locus_tag="ISB30_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAP1990389.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLNKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADNVRD"
     gene            complement(7788..8399)
                     /gene="emtA"
                     /locus_tag="ISB30_002776"
     CDS             complement(7788..8399)
                     /gene="emtA"
                     /locus_tag="ISB30_002776"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAP1990390.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8499..9413
                     /gene="ldcA"
                     /locus_tag="ISB30_002777"
     CDS             8499..9413
                     /gene="ldcA"
                     /locus_tag="ISB30_002777"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAP1990391.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTEEGTQLTISGHPVLKM"
     gene            9508..11244
                     /locus_tag="ISB30_002778"
     CDS             9508..11244
                     /locus_tag="ISB30_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAP1990392.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11301..12371)
                     /gene="dadX"
                     /locus_tag="ISB30_002779"
     CDS             complement(11301..12371)
                     /gene="dadX"
                     /locus_tag="ISB30_002779"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAP1990393.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHQEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12381..13679)
                     /locus_tag="ISB30_002780"
     CDS             complement(12381..13679)
                     /locus_tag="ISB30_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAP1990394.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVDQAAFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEALSVARYSRGFTPSRPGHLHGAHS"
     gene            14009..15541
                     /locus_tag="ISB30_002781"
     CDS             14009..15541
                     /locus_tag="ISB30_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAP1990395.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(15593..16312)
                     /gene="fadR"
                     /locus_tag="ISB30_002782"
     CDS             complement(15593..16312)
                     /gene="fadR"
                     /locus_tag="ISB30_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAP1990396.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            16534..18075
                     /gene="nhaB"
                     /locus_tag="ISB30_002783"
     CDS             16534..18075
                     /gene="nhaB"
                     /locus_tag="ISB30_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAP1990397.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAALLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRNQRTRQDKIRLIVQAIIGVWLVTALALHLVEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTVAGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            18221..18751
                     /gene="dsbB"
                     /locus_tag="ISB30_002784"
     CDS             18221..18751
                     /gene="dsbB"
                     /locus_tag="ISB30_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAP1990398.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(18796..20064)
                     /locus_tag="ISB30_002785"
     CDS             complement(18796..20064)
                     /locus_tag="ISB30_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP1990399.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(20064..20483)
                     /gene="umuD"
                     /locus_tag="ISB30_002786"
     CDS             complement(20064..20483)
                     /gene="umuD"
                     /locus_tag="ISB30_002786"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAP1990400.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIIIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            20680..>21004
                     /locus_tag="ISB30_002787"
     CDS             20680..>21004
                     /locus_tag="ISB30_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
                     /protein_id="HAP1990401.1"
                     /translation="MEAFAQKGILIKVLDDGIKKLCSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETARFYVDYDDLMLSLLKE
                     AAQKMI"
BASE COUNT         4965 a         5501 c         5310 g         5228 t
ORIGIN      
        1 aagtgttatt taagaaacaa gcaaatcatt ctgtctcttt agacaaattt tgacatcaaa
       61 catgatcttt ttcgctatat gtgtaagtat atagcggttt taattatcag gatattactt
      121 atgtcactaa ccagacggag gtttacacag attcttgcgt cgacattgtt cctgcatcat
      181 ctgccgtcct ttgcacaatc agtcaaattc tgggcctcac tgacgcttcc cgaagcccaa
      241 cacattacac gcatcgtcag cgcaggcgcg cccgccgatt tactattgct ggctgtcgcg
      301 ccagaaaaaa tggtcggttt ttcctctttt gattttgccc gtcaggcatt aattccattg
      361 ccagagcaca ttcgccagtt ccccaggctg ggacgactcg ccgggcgcgc cagcacactc
      421 tcgctggaag ggcttatggc gttacatccc gatttggtcg ttgattgcgg caatacggat
      481 gaaacctgga tctcccaggc acggcaggtt agcgaacaga cacaaatacc ctggttattg
      541 cttaacggga aactggaaca atcagcagaa cagttaacaa cgcttggcaa aacgttaggc
      601 gaagagcacc gcgccgcaga acaagccaat ctcgccagcc gcttcgttgg tgaagctcag
      661 gcattcgcca cctcacccgc cgctaatctc agcttttatg ctgcgcgcgg tcctcgaggg
      721 ctggaaacgg gcttacaggg ttcgttgcat accgaggcgg cggaattatt aggtttgcac
      781 aacgtcgcgc aaatagccga tcgccacggt ctgacacagg tttccatgga aaatctcctg
      841 cgctggcagc cggatattat tctggttcag gaggccgtta ctgcagattt tattcgtcgt
      901 gatcctctct ggcagggcgt gaaagcggtt gcggaacaac gcatcttatt tttaagtggc
      961 ctgccctttg gctggctgga tgccccgccg ggaatcaacc gtcttctggg attacgcaga
     1021 cttcacgcct ggctggatcc cgccatcaat cgccagttta aaagtgacat gcagcattac
     1081 gcccaactgt tctggcattg ttcactcagt gacgccgact atcaaaaatt ggtggcgagc
     1141 taatgagaac cgtaaacgga tgtattttgc tgctggcagc tatcagtatc acttttgccg
     1201 ccgtaagtgg ggcttatcat ctcgatatgc agcaactgct ggcgctcatt cttcgtcaag
     1261 aaaatgttcc tgtgcaggaa caaattgtct tctggcaaat ccgcgtgccg cgtattcttg
     1321 ctgcgctgtt tctgggcgcg gcgctggcag gggccggaac cacctatcag ggaatgttcc
     1381 gtaatccctt agtgtcaccg gatattttag gcgtctccgc cggtgccgga cttggtgcct
     1441 gcgcggcaat cctgtgggga ttatccattg tttacatcca gctgtacgca ttttgcggcg
     1501 ggctgatggt ggtcgcgggc gtctggttga ttacgcgtcg ggtgacccgc cacgatccga
     1561 tcttaaccct ggtgctggta ggcattgcgt taggaacgtt gtgcggagcg gggatatcgc
     1621 taataaaaac gctggccgat ccttacaccc agttaccgtc aattaccttc tggttgctgg
     1681 gggggctgtc caccgtgacc ttacgcgatc tctgctatgc tgcaccgatc attctcatag
     1741 gctccttgcc cctgtttttt ctccgctggc gcatgaattt actcacgctt tccgacgacg
     1801 aagcccgttc gctgggactg aacgtgacac gtctgcgctt tggattgatt gtctgcgcca
     1861 cactcatcac cgccagcacg gtagcaatag caggcataat tggttgggtc ggcctggtcg
     1921 ttccgcacat tgcccgcttg ttgaccggtc ataaccatca gcagctgttg cccatggcaa
     1981 tgtgtactgg cgcgattctg ctcctgctta ccgatacgct ggcacgcagc attggaacaa
     2041 cagaaatccc acttggcatt ctgaccgctt ttgttggtgc accgtttttc ctgtttttgt
     2101 tgttacgcgg aggtcgccag tgactttgct tgccgtccgt catgcgtctc tgggttactc
     2161 ccgccatccg gtgctacgcg acgtgtcctt caccctgtcg caaggaacaa tttgttgtct
     2221 gttaggagcc aatggttgcg gcaaaaccac actcatgcgc tccattcttg gcgttatacc
     2281 gttattaaaa ggcgagatct tactggatag cctccctgtg cagacactca gccatcgcca
     2341 gcgcgcgcag gctattgcct gggtgccgca ggcgcacgac ggcatttttg cttttagtgt
     2401 gctggatatg gtgttgatgg gcctggcccc caccataggc gctttttccg ttcccggtaa
     2461 gcaagaacgc ctgaaagcaa cggagcaact ggaaaagctg ggcattttgc atctggcgac
     2521 acgacgctgg aatacgctta gcggcggtga acgtcagctg gctttaatcg cccgtgcact
     2581 cgtccagcaa ccgcgcttgt tattactgga tgagcctgcc tccagcctcg atttcggtca
     2641 tcagatccaa cttctggaca cgctggcgca gctcaaaaat aacggcatga caatgttaat
     2701 gtcgactcat catccgctcc atgcaaacgc cattgccgac agcattatcc aggtagagcc
     2761 agatggacga gtaacacaag gcttaccgac cgaacagtta acaacgaaaa aactggctgc
     2821 cctgtaccgg gtttctgccg accagattca ccatcacctc tctgcaatta agcactaagg
     2881 aaaaatcatg ctcattgacg atattgattt tgcagacctt taccttcagc aactcaggct
     2941 ggcccatcgc acagaaaaaa cgccggatca ctgggatcaa cgggcggaaa aaatggcgga
     3001 aaattgcgcc tcgcctacgg acagctacct gcaacaactg acctcaaaaa ttgacctgca
     3061 aggcgcgcaa accttattcg atatgggctg cggaccgggt accgtatcgc tggcgctggc
     3121 ggacaaactc acgacgattt atggcgttga ttacagtcag gggatgctga acgttgccgc
     3181 ccgtcgtgcc gccgcgctaa aggcagacaa tgtccactgg atccagcgcg cctgggaaga
     3241 ggactggagt gacctgccac gctgcgatat tgccgtggct tcccgctcca ctctggtggc
     3301 tgatatgcgc caggcaatga gcaaactgaa taatcaggca cggcttcgcg tctatacgac
     3361 tcatctggtc agcacctcgt tcgtctctcc agccattcag cgggcggctg gtcgggaagt
     3421 gatcgaatta cccaactata tctttgcact caacgtcctg tatcagatgg gaatttatgc
     3481 tcacgttgat tttatccgcg gacaaaattg ccaacaggat aacagcacct gggaacgttt
     3541 tgagcagaac gtcagctgga gtctgggcgc gcttaacgac gatgagcgtg agcggttata
     3601 tcgctggtat caacaacaag atgctcgtgc cctcgcccct gccagccgcg actgggcgct
     3661 catctggtgg gatagcgtac cgcaggagac actgcgatga ttttcctttc tcaggcacaa
     3721 atcgatgcgt tattgctgga agatatccag ggcggtgacc tgaccacgcg agcattaaat
     3781 attggacacc agcatggcta tatagagttt tttctccgtc agggcggctg catcagcggc
     3841 atttctgtcg cgtgtaagat gttaactacg ctgggattaa ctattgatga cgcggtcagc
     3901 gacggttcac aagcgaacgc gggtcagcgg ctaatccgtg cgcaaggtaa tgccgcagca
     3961 cttcatcagg gatggaaggc agtccagaat gtgctggagt ggagttgcgg tgtttctgat
     4021 tatctcgctc aaatgctggc gttacttcgt gaacgttacc ctgatggcaa tatcgcctgc
     4081 acccgaaaag caattccggg cacccgctta ctggcctcgc aggcaattct ggctgccgga
     4141 ggactgattc atcgtgccgg atgtgcggaa accatattat tgtttgccaa ccaccgccat
     4201 tttcttcatg acaatcagga ctggtcaggc gcaatcaatc agttacgtcg ccacgcaccc
     4261 gagaagaaaa tagttgttga agccgacacg ccgaaagagg caatcgccgc gttacgcgcg
     4321 caaccagacg tacttcagct cgacaaattt agtccgcaac aggcaactga aattgcgcaa
     4381 atagcaccgt cgctggctcc ccactgcacg ctggctctga ccggcggaat taatctgaca
     4441 acactcaaaa attacctcga ctgcggcatt cgccttttta ttacctccgc gccttattac
     4501 gcgacaccag ctgacattaa agtcagtctg caacccgcag cctctattta attctttgtt
     4561 aaacggaatt tattctatga gactcaaaaa acgttatctc tgcacagcat tatcacttgc
     4621 ctttacccag caggccgtag cggctcagga gagtgacacg ctgaccgtat ggtccagtcc
     4681 ggtatcatca acgacgacca ccgttctcga tcaacccacc atgaaggccc tggataaaca
     4741 gaatgtcgct caggcattaa gtgtcgtccc cggcgtggtg ctgcaaaagt caggtagccg
     4801 caacgaagaa caggtcaaag ttcgtggctt tgatagtcgt caggtgccgg tctatttcga
     4861 cggtgtgccc atttatgttc cctatgacgg caacctcgat ctggcgcgga ttctgaccaa
     4921 caatttgggg gcagttgaag tttccaaagg gtattcgtcg ctgcttcagg ggcctaatca
     4981 gatgggcgga gccattaata tcaccaccca gaagccaaca aaacctctgg aagcaagtct
     5041 gggatatcgc cagggatgga gccgtagcca ggacaatgcc tacgatatgc atgcttcatt
     5101 tgccgccagc agcgatctgg ggtatttgca agtcagcggt agccagctaa agcaggattt
     5161 tctcggcctg ccgcatggtg taaataatga tattgcaggc aaacacggca agatgattaa
     5221 ttcatcggct gatgataaac gcggcattgt gaagctcggt tttacaccac gcgaaaacga
     5281 tgaatacaca ttgacttaca ttaagcagga tggtgaaaaa gataacccgc catacagtgg
     5341 aaatagtggt cagaaatcac gctactggca gtggccagag tatgacaaag aaagttttta
     5401 ttatcaggga acgacccaac taaacgatcg ttttaccctg aaaagtcggc tgtatcgcga
     5461 cacctttgaa aatacgctaa tgatgtacaa ctcgctggct gatttgaaaa ataaaaaagg
     5521 cagctacagc cattattccg attacagcga cggtgccggg ttacaactgg cagccgatgt
     5581 gcgtgaaaac gatctgctgt cgtttgccgt taactggaaa gatgacgtac accgggaaaa
     5641 aggtgcgccg cacgccgctt acgatcgcta tgaagatcgt acctggtcgc tcgccagtga
     5701 atatcaatgg gctgctgccg ataatgtcga tgtcgtggct ggaatcagct atgactggcg
     5761 cgatagcgta gaagcgaaaa aacatgagaa agatggcagt atcacccatt atgacgacaa
     5821 caatcagtca gcttttaact ggcaggtgat ggggaaatac cactttgcca atgaagacac
     5881 gctggcgctt tcgtactatg accgcacacg ctttccgacg ctgaaagaac gctataccac
     5941 gtccaaacct gcgtataacc agatagcgat tgttaacccg cagctcaagc cggaacgcgc
     6001 gcgcggggtg gatttaacct ggaatggtgc cttcacgcac gactggggat ttgaggtcag
     6061 cgtttactat aaccgggtga gtgatgccat cctctcgcac aatatcgatg ccgataccat
     6121 tcaaaatcag aacagcggca cggtggatta cagcggtctg gatgccggta ttaaggggaa
     6181 aatcagcaat atactggatg taggattgag ctacgccctg atccacgctg atgccaaacg
     6241 caaagacatc ggcaagataa ccgatctgcc aacgcagaca atgaccgcat ggatgactct
     6301 caaaccgtgg gagccgttaa gcgtaacgct gtcggaagag gcgcgttcct ccagctacag
     6361 caacagtgac ggttcacaaa aagccgccgg ttttgcggtg actcacattc gagccgatta
     6421 caccttaggt catggcttca gcgttaatgc gtcggtcaat aacctgtttg ataccaaata
     6481 tgcctacagt gaagggttta ttgaagaagg tcgcaatttc tgggcaggta tcgagtatac
     6541 gttctgatgt gaaagcggat agcggcatct gtatcgctat ccgccttatg aaaatcttat
     6601 tctcttcgca ggaggcggaa tacgcccagc accaggatag cgcccaccac cgccaccagg
     6661 aagctgtgca aattaaaacc gctgatggag ccgccaatgc caaacatggt cgccagccag
     6721 ccgccgacca ccgcaccgac tatcccaaga atacaggtca ggaaaaatcc accaccatct
     6781 cgccccggca tgattagctt ggcgataatg ccggctatca ggccaaaaat aatccaggca
     6841 ataattccca tgttcaggcc ctcgtttcga tgaatcggac gcaagaaatg cttacgtcaa
     6901 cgctctaagt ataggcaagg gcgggatcga tgctgggcat atgtcggctg atttaagaac
     6961 aacatgtaaa aaatattcgc gcatttgtat taagttttgt taactgcgac cgataaacca
     7021 aagacagttt gtcagtcagg agtttttccg cgtgagtcat taccatgagc agttcctgaa
     7081 acaaaatccg ttagccgtcc tgggcgtgtt acgcgatttg aacaaagccg caattccttt
     7141 gcgtatcagc tggaatggcg ggcagctgat cagcaaaata ctggcaataa ctccggataa
     7201 actggtgctg gatttcggca gccaggccga agacaacatc gccgtgctaa aggcgcagca
     7261 cattaccatt accgccgaaa ctcagggtgc gaaagtcgag tttactgttg aacaactaca
     7321 gcagagtgaa tacttgcagc ttccggcatt tattaccgta ccgcctccca ccttatggtt
     7381 tgtacaacga cgccgatatt tccgcatcac cgccccactc catccgcctt atttttgcca
     7441 gaccaaactg gcggataaca gtacgttacg tttccgcctg tatgatttgt cgttaggcgg
     7501 catgggcgca ttactggaaa cagcaaagcc tgccgaatta cacgaaggca tgcgcttcgc
     7561 tcagattgaa gtcaacatgg ggcaatgggg tgtttttcac tttgacgccc agttaatctc
     7621 catcagcgag cgcaaagtga ttgatggcaa aaatgaaacc atcaccactc cccgtctgag
     7681 cttccgtttt cttaacgtca gcccgacggt ggagcggcaa ttacagcgga ttattttctc
     7741 tctcgagcga gaagcccgga aaaaagcgga caatgtgcgt gactgattta catcgcgtcc
     7801 agtgcctgct caagtttgta gatatagcgc ggagcctgcg gcgcaggatg atttcgcgct
     7861 acgtgatcga ggaactcatc agcatccaga tcgttgattt tgctgatcgc ctttttccga
     7921 tctgacgaga aagtccgtag cagcgcacct gccccgttag cgtatgacac caccagcgca
     7981 tattgcagta ccttcggatc ttcaatgcct gccagcgggc cggtttccag aatattcagg
     8041 taagccgctc ccattgaaat attacgctcc ggatttttca gctcgctggt cgtcggctca
     8101 ccgctccagc ccatacgacg atatacatca cgtccggagg ttgaagcttt tatctgcatc
     8161 aaaccaatgg cattcgattt actcaccgcg ttcggattac cacccgattc gatagcgata
     8221 atcgccgtga tcaattgtgg atcgacgccc caggctgcac cggctttctg gcttattggc
     8281 atccactgca tcgcacgttg caccggaact ttcgcgttcc acggcgggtt cgtatagtca
     8341 tgctttgatg aacaacccgc taataacaca atcaaaaagg caaaccatct caatttcact
     8401 tcttctatcc ttatagcctg gaatcgccgt tgaggtgaca acggcacacc taagcggcat
     8461 gatatacatt tcgtttctgt tgtcagcaag gaattgccat gtctctgttt cacttaattg
     8521 ccccatcggg ttactgcatt aaacagcacg ccgcgctgcg tggtatccag cgccttaccg
     8581 acgcggggca tcaggtcaat aacgtcgagg ttattgcccg tcgttgtgag cgttttgcgg
     8641 gtacggaaac ggagcgtctg gaagatctca attctcttgc cagactgact acccccaaca
     8701 ccatcgtgct ggctgtacgc ggcggttacg gtgccagtcg tttactggca gatattgact
     8761 ggcaggcact ggtggctcgc caacaacatg acccgttact catttgcgga catagcgatt
     8821 ttaccgccat tcagtgcggt cttctggcgc aaggcaatgt catcaccttt agcggcccca
     8881 tgctggtagc aaactttggc gcggatgaac taaatgcctt taccgagcac cacttctggc
     8941 tggcgttacg caataaaacg ttcaccattg aatggcaagg ggaaggcccg acatgtcaga
     9001 ccgaaggcac gctgtgggga ggcaatcttg cgatgctgat ttcactgatt ggtacaccgt
     9061 ggatgccaaa aattgagaac ggtattctgg tgctggaaga tattaacgag caccctttcc
     9121 gcgtcgaacg catgctgttg cagctctatc atgctggaat tttgccacgt cagaaggcga
     9181 ttattctcgg tagctttagc ggcagcacac ctaatgatta tgacgcaggt tacaacctgg
     9241 agtcagtgta cgcgtttttg cgttcccgcc tgtcgattcc actcattacc ggcctcgatt
     9301 ttggtcatga acaacgcacg gttacgctgc cgttgggcgc acatgccatt ctgaataaca
     9361 ccgaggaagg cactcagttg acgattagcg gtcatcctgt tcttaaaatg taaaaaaatc
     9421 acgcttcagg gctaagtaaa acgctgtctc tgccgctaat atggtagggt ttatatcaga
     9481 gacagcgtaa tcaggagtaa ccgaccgttg gatgccacaa caataattag ccttttcatc
     9541 ttaggatcca tccttgtcac cagcagtata ttacttagtt cattttcttc ccgtcttggc
     9601 attcctattc tggttatttt tctggcgatc ggcatgttgg cgggggttga tggcgtcggc
     9661 ggtatcccgt ttgataatta ccccttcgcc tacatggtaa gtaacctggc gctggcgatt
     9721 attttgctcg acggcgggat gcgcactcag gccagttcct ttcgtgtggc gttaggaccg
     9781 gcactgtcgc tggcgacgct gggcgtactt atcacctctg gtttaaccgg catgatggcg
     9841 gcgtggctgt ttaatcttga tttgattgaa ggattattaa tcggcgctat cgtcggctcc
     9901 accgatgctg cagcggtctt ttctctgctg ggtggtaagg ggcttaacga acgtgttggc
     9961 tcgacgctgg aaattgaatc cggcagtaat gatccaatgg cggtctttct gacgattact
    10021 ctgattgcga tgatccagca gcatgaaagc agtgtcagct ggatgttcgt ggtcgatatt
    10081 ctgcagcaat ttggcctcgg gattgtcatc gggcttggcg gtggttattt actgctgcaa
    10141 atgattaatc gcatcgccct gcccgccgga ttatatccat tgctggcatt aagcggcggt
    10201 attttaattt ttgctttaac cacggcgctg gaaggcagcg gtattctggc tgtttatctg
    10261 tgcggttttc tgctgggtaa tcggccgatt cgcaaccgct acggcatcct gcaaaatttc
    10321 gacggcctcg cctggctggc gcaaatcgcc atgttcctgg tgctggggct attggttaac
    10381 ccaagcgatc tgctgcctat tgccattccg gcgctcattt tgtccgcatg gatgattttc
    10441 ttcgcccgtc ctctttcggt atttgccgga ttgctaccgt tccgtggctt caatctgcgt
    10501 gagcgcgtgt ttatcagctg ggtaggatta cgcggcgcgg tgccgattat tctggctgtg
    10561 ttcccgatga tggcggggct ggagaatgcg cgactgttct ttaatgtcgc cttctttgtg
    10621 gttctggttt cactgctatt gcagggaaca tcactctcgt gggcggcgaa aaaagccaaa
    10681 gtggtcgttc cgccagtggg acgtccggtg tcacgcgttg gcctggatat tcatccggaa
    10741 aatccgtggg agcagtttgt ttatcaattg agtgccgata aatggtgcgt gggcgcggca
    10801 ctgcgtgatt tgcatatgcc aaaagagacg cgtattgcgg cactgtttcg tgataaccag
    10861 ttgcttcatc ccaccggcag cacccgactg cgcgaaggcg atgtgttgtg tgtaattggt
    10921 cgggaacgcg atctcccggc gctcggtaaa ctgttcagcc agtcgccgcc ggtcgcgctg
    10981 gatcaacgct tctttggtga cttcattctc gaagccagcg ccaaatatgc tgatgtggcg
    11041 ctgatatatg gtctggaaga cgggcgagaa tatcgcgata agcagcaaac gctgggtgaa
    11101 atcgtccagc agttgttagg cgcggcaccg gttgtcggtg accaggtaga gtttgccggg
    11161 atgatctgga cggtggccga gaaagaagat aatgaagtgt tgaagattgg tgttcgggta
    11221 gcggaggaag aagccgaatc ttaagtttaa tgtgtttcgt aacgctggat acgacgctgg
    11281 tcgcatccag cgataacaag ttacaccgtc acaaccggga cgcgtaacgc cagcgcgcac
    11341 atcaactcat agcctaccgt tccggcagcg gcggcgacat catcaatttt gatctccttg
    11401 ccccacagct caactggcgt accaataccc gcttgcgggc aaggcgttaa atcgaccgcc
    11461 agcatatcca tcgagactgt ccccaccgtc atggtgcgaa cgccgtccac taaaacaggg
    11521 gtaccggtag gcgcgtggcg cggataaccg tcggcgtatc ctgcggcgac aatgccgatt
    11581 cgctgttcat cgcgcgcagt atagcgaccg ccgtagccca cacgctcgcc cgcttttagc
    11641 gtctggacac caataatctc actgcttagc gtcatcaccg gacgtaatcc ggtattggcg
    11701 atatcacgcc actgaccgga cggcgaagca ccatacaaaa taatgccagg ccgaacccag
    11761 tcaaaatgcg cttcctggtg ccacagagtc gccgccgaat tagacaacga acgccgacac
    11821 tccagccctt ccgccgcctg ctcaatacgc gccatcgcgc tggaaattcc atcaggatgt
    11881 tccgcttcgg caaaatgcga catcagggtc atttcgccaa cattcgccat cgcccgcaac
    11941 tgctgccaga cggtaagcac gcgatcgggc tggaagccca accgattcat cccactgttc
    12001 actttaagat aaatatccaa cggtgctttt agccgcgcat tttgcagtgc tttgagctgc
    12061 cagttgctgt gtacgcaggt ggtcaggcgg tgctggtcat aaatctccag atcctgagca
    12121 tggaaaaatc cttccagcat caggatcggt cctttccagc cgcgctcacg taacgttatt
    12181 gcctcttcca ggttaagcaa tgcaaagcca tcggtagccc cgagcgcgct ccagatacgc
    12241 tcaataccgt gcccgtaagc gttcgctttt accaccgacc agacgcgcgc gtgcggcgcg
    12301 gcctggcgga caatggacag attctgtttt aatgcctgca gatcgaggct ggcctgtatc
    12361 ggacgggtca tctcgtttcc ttagctgtgt gcgccatgta aatggcccgg gcgtgatggc
    12421 gtaaatccac ggctgtagcg cgctacgctt agagcttcat acgggatcgc tggcgtgcgc
    12481 cccgagagca gatcgcttaa caactgaccg gaaccgcaag ccatcgtcca gccgagcgtg
    12541 ccgtgaccgg tattcaacca cagattttta aagcgtgtac gcccgacaac cggcgtgccg
    12601 tctggcgtca tcgggcgcag gccagtccag aaagccgcct gatcaacatg accgccgcgt
    12661 ggatagagat cgcgaaccac catctccagc gtttcacgac gcggttgcaa cagctcggtg
    12721 ttaaaaccaa caatttccgc cattccgccg acacgaatgc ggttatcgaa acgggtaatg
    12781 gcgattttgt aggtttcatc aagaatagtg gataccggcg caccatcttc ctgcgcaatt
    12841 ggaatggtca gcgagtagcc tttcagcgga tacaccggaa tatcaacaat gcctttgagc
    12901 atcgccgtcg agtaagaacc aaacgccatc acatacgcat cggccttaat cacttcatca
    12961 ccacacttca cgccgtagat ttgctcgccg tcgcaaagca gttggtcaac gggcgtatta
    13021 aagcggaatt taacccccgc ctgctccgcc atccgcgcca gattctgggt aaacagctgg
    13081 cagtctccgg tttcatcatt gggcagttgc aggccgcccg tcagtttgtg cgctacttct
    13141 gccaacgcgg gctccacttc cgccaggcgg ctggattcca gcagctgata cggtacgcca
    13201 gcatcttcca gcacggcgat atcgcgggtc gcgttttcat actgttgttc ggtacggaac
    13261 agttgcagcg tcccaccctg acgcccttca tactgaatat tggtttcggc gcgcaatgct
    13321 ttcaggcaat cacggctgta ttccgccaga cgtaccatcc gccctttgtt ttccatgtag
    13381 tggctggtgt cgcagttacg taacatttgc cacatccatt tcaactggaa ctgcgtaccg
    13441 tcgagacgaa ccgccagcgg cgcatggcgc tggaacatcc atttaatcgc ttttaaaggc
    13501 acacctggtg ccgcccacgg tgcagcatat ccgggggaga tttgcccggc attcgcggca
    13561 ctggtttcca gggcggcccc cggctcccga tcaatgacgg tgacctcatg tcctgcctga
    13621 tttaagtacc aggcgctggc aacgcctacc acaccacttc ccagtatgac aactcgcata
    13681 gccactccga taacggtaga tacagtaaaa gaataataat ctaattacat cttgataact
    13741 cagttgaaaa tattattcaa catacggctt ttttatggtg agctggctca catctccctg
    13801 actctgcggc aatatggcgt ctttagtatc tcctgctgaa cgcccttttt atccagcata
    13861 aaattccgtt cagaagcgga ttagtggcac tctgataata agaaatcgca aagaaaaaaa
    13921 ttctccggcg acgcggtttt taacaggtgt tctatgcttg aaatgaggtg ctccacacag
    13981 agagcgccga caacaatgag gtgcgcgtat ggcgacgatc gattctatga ataaggacac
    14041 cacacgtttg agcgatggac ccgactggac gttcgacctg ctggatgttt atctggcaga
    14101 gatagaccgg gtggcgaaac tctaccggct ggatacctac ccgcaccaga ttgaagtgat
    14161 aacctcagaa cagatgatgg atgcttactc cagcgtcggc atgccaatta actatccgca
    14221 ctggtcattc ggtaaaaagt ttatcgagac tgaacggctg tataagcacg gtcagcaagg
    14281 actggcctat gaaatcgtca ttaactctaa cccgtgtatc gcttacctga tggaagagaa
    14341 caccattacc atgcaagcgt tggtgatggc tcatgcctgc tatgggcata actctttctt
    14401 caaaaacaat tacttattcc gtagctggac cgacgccagt tcgattgtcg attatctgat
    14461 ttttgcccgt aaatatatta ccgagtgcga agaacgttat ggcgttgatg aagtagaacg
    14521 gcttctggac tcgtgccacg cgctgatgaa ctacggcgtg gaccggtaca aacgcccgca
    14581 aaaaatctcg ctgcaagaag agaaagcccg gcagaagagt cgcgaagagt atctgcaaag
    14641 tcaggtcaat atgctctggc gtaccctgcc gaagcgcgag gaagagaaaa cggttgctga
    14701 agcgcgccgc tatccgtccg aaccacaaga aaacctgctc tattttatgg agaaaaatgc
    14761 gccactgctg gaatcatggc agcgtgaaat cctgcgtatt gtgcgtaagg tgagccagta
    14821 tttttatccg caaaaacaga ctcaggtgat gaacgaaggc tgggcgacct tctggcacta
    14881 caccatcctt aaccatctgt atgatgaagg gaaagtaacg gaacgtttta tgctggagtt
    14941 tttgcacagc cacaccaatg tggtcttcca gccgccctat aacagcccgt ggtacagcgg
    15001 catcaaccca tatgccctcg ggttcgccat gttccaggat attaaacgga tttgtcagtc
    15061 gccgacggaa gaagacaaat actggttccc ggatatcgcc ggttccgact ggctggaaac
    15121 gctgcatttc gcgatgcgtg atttcaaaga tgagagtttt atcagccagt tcctgtcacc
    15181 gaaagtgatg cgtgatttcc gcttcttcac cgtactggat gatgatcgac ataattatct
    15241 ggagatttcc gctattcata atgaagaagg ttatcgggaa atccgtaacc ggttatcgtc
    15301 gcaatataac ttaagtaatc tggagccgaa tattcagatc tggaacgtgg atttgcgcgg
    15361 tgaccgttcg ctgacgctgc gttacattcc acataatcgc gcaccgttgg atcgggggcg
    15421 caaagaagtg ctgaagcatg tgcatcgcct gtggggattt gatgtgatgc tggaacagca
    15481 aaacgaagac ggcagcatcg agttgctgga acgttgcccg ccgagaatgg gaaatctgta
    15541 aaaacaacga aaaacccctc gtttgagggg tttgcttttt aaacggaagg gattatcgtc
    15601 cctgaatggc taaatcaccc ggcagatttt tctgcatccg gtgccaaatc tcgccactct
    15661 catgcccata gcgacgcact gtttcatata cctgatcgtg tgcgccttca ctgcacaacg
    15721 ccgacagttt gtgatagaag cccagcgcca gactgcgcgc ttccggattg gcgaaatagt
    15781 gacgaccaat gcgcgtatac agccctttca tcccgttaag gatcagaccg taaatcgggt
    15841 tgccggaagc aaacgccagg ccgcggaaaa tgttgtaatc cagctcggca aaggcgtcgg
    15901 cgtgatcggc cacttcatta gcggttgcca gcacttcctg cgctttatcg ggatgttgac
    15961 gaaacgcggt gcgaataaaa atagttgaaa tattggtacg caccgacagc aaattatcaa
    16021 taagctgcgg cacactttcg tggtccagtc gcgccagtgt ttcaaggata tttaaaccag
    16081 aggtttccca gaaattattc accttcgtcg gcttgccgtg ttgaatggtc aaccagccat
    16141 ctcgtgccag acgttgtaac acttcacgta acgtagtacg cgttacgcca attaattctg
    16201 aaagttcacg ttctgcgggc aaaatagtcc cgggagggaa gcggttattc cagatacttt
    16261 caataatgta ctcttccgcg aaacccgccg ggctttgcgc cttaatgacc atagtgagat
    16321 ttccataaca cagcaaaaca aagttggact catcatacca gagggctaac tacgctgata
    16381 gaagacaaaa agaagaaaaa agggatcaag gcttcatttt ctgcatctct ttccccttga
    16441 ctatcaagac ttgcctcact cctgtctgac cgttaagctt tgcgtttaaa cataaaaaca
    16501 tcattgatta tttctaaggt aagggaaacc atcatggaga tctcctgggg ccgcgcacta
    16561 tggcgcaatt ttttgggcca gtcccccgac tggtacaaac tcgccctcat cattttctta
    16621 atcgtaaatc cgttaatttt cctcatcagc cctttcgtcg ctggctggtt gctggtagcg
    16681 gaatttattt tcactctggc gatggccctg aaatgctacc cgctgctccc cggtggtctg
    16741 ttggctatcg aagcggtatt catcggcatg accagcgcgg aacatgttcg tgaagaagtg
    16801 gcggcaaatc ttgaagtctt gctgttactg atgtttatgg tggcgggtat ctattttatg
    16861 aaacagctgt tgttgttcat atttacccgt ttgctgttaa gcattcgctc caaaatgctg
    16921 ctgtcgctct ctttttgcgt ggcggcggcg ctcctctccg cgttcctcga tgccttaacc
    16981 gtcgtggcgg tggtgatcag cgttgcagtc ggtttttatg gtatttatca ccgcgtagcc
    17041 tcttcccgta ctgaagatac cgacctgcaa gacgatagtc atatcgacaa gcattacaaa
    17101 gtggttctgg aacagttccg tggctttctg cgtagtctga tgatgcatgc cggtgtcggc
    17161 accgcattag gcggcgtgat gaccatggtg ggcgaaccac agaacctgat catcgctaaa
    17221 gcggctggct ggcattttgg cgatttcttc ctgcgcatgt cgccggtgac cgtcccggtt
    17281 ctgatttgtg gcctgttaac ctgcctgctg gtagagaagc tgcgttggtt tggctacggt
    17341 gaaacgctgc cggagaaagt ccgcgaagta ctgcaacagt ttgacgatca aagccgcaac
    17401 cagcgtaccc gtcaggataa aatccgtctg attgtccagg cgattatcgg cgtctggctg
    17461 gtgactgcgc tggcactgca tctggtggaa gttggtttga ttggtttgtc agtcattatt
    17521 ctggcgacat cattgaccgg tgttaccgat gaacatgcta tcggtaaagc cttcaccgaa
    17581 tctctgccat tcaccgcact gttgacggtg tttttctcag tagtcgcggt gattatcgac
    17641 caacaactgt tttcgccgat tattcagttt gtgttgcagg catctgaaca tgctcagctg
    17701 tcgttgttct atattttcaa cggtctgctg tcatccattt cggataacgt cttcgtgggg
    17761 acgatttata tcaacgaagc gaaagcggca atggaaagtg gcgctatcac gttgaagcaa
    17821 tatgagctgc tggcggtcgc cattaatacc ggtaccaatc tgccctccgt cgctacgccg
    17881 aacggtcagg ctgcgttcct gttcctgctg acctctgcac tcgcgccatt gattcgtctc
    17941 tcttatggcc gcatggtgtg gatggccctg ccttacaccc tcgtactgac ggttgccggg
    18001 ttactctgcg tcgagtttac gcttgctcct gtaaccgaat ggtttatgca aatgggctgg
    18061 atagcaacgc tttgataaca acttaccggg catataaatg cccggtttgc cttttcgccc
    18121 gataattgtc caattgcgtc ctatttttgc tgcctcctgg tggcgcagcg aatgaattgg
    18181 tttaaactgc gcactctatg catattgcag ggaaatgatt atgttgcgat ttttgaacca
    18241 atgttcacaa ggccggggcg catggctgtt gatggcgttt actgctctgg cactggaact
    18301 gacggcgctg tggttccagc atgtgatgtt actgaaacct tgcgtgctct gtatttatga
    18361 acgctgcgcg ttattcggcg ttctgggtgc tgcgctgatt ggcgcgatcg ccccgaaaac
    18421 tccgctgcgt tatgtagcga tggttatctg gttgtatagt gcgttccgcg gtgtgcagtt
    18481 aacttacgag cacaccatgc ttcagctcta tccttcgccg tttgcgacct gtgattttat
    18541 ggctcgtttc ccggaatggc tgccgctgga taaatgggtg ccgcaagtgt ttgtcgcctc
    18601 tggcgattgc gccgagcgtc agtgggaatt tttaggtctg gaaatgccgc agtggctgct
    18661 cggtattttt atcgcttacc tgattgtcgc ggtgctggtg gtgatttccc agccgtttaa
    18721 agcgaaaaaa cgtgacctgt tcggtcgcta atccattcgg cgctcctgcg ggagcgcttt
    18781 ttcctgccgc tatgtttatt tgacccgcag taaatcagaa cttcgcgttg tataacatgg
    18841 tgaaagcatg gcacgcttca tctgccattg ttgctggatc ccctgcccgg caaaatagag
    18901 tgttcctctg ccctctttag cattcagcgt atccattacc gccatcaatt gctcactccc
    18961 ggggcgtggt gcgttgtcat cgaataaatt gagctgcgcg actccctgac tgaagaaatc
    19021 ccccagcatc acgcccgctt tttggtagcg atggcccgct tgccagatgg catccagaga
    19081 tcgcgtagcg gcgttaatga tatccctgct gtcctgagtg ggcgtcagca gttttaccga
    19141 tgcgctattg ccgtaataag gttcattgaa cgcaaatggt gacgtcttaa taaacgtgga
    19201 gataaaccga caatattgat gctcgctgcg aagtttttcc gccgcccggg cagcgtaact
    19261 acaaatggcc tgccgcatcg acggataatc cgtgatgcgt tcaccaaacg agcgggaaca
    19321 gataatttcc tgcttcgtcg gtgcaaactc ttccagttgc aaacagggtt caccgcgcag
    19381 ttcacgcacc gttctttcga gcacgacatt aaaatgttta cggataaacc ggatatctga
    19441 atctgccaaa tctagaacgg ttttgatccc catcgcgtcc agttttttgc tgatccgccg
    19501 tccaatcccc cagacttcat ccacggggag agcagacatt aatttacgct ggcgttccag
    19561 atttgataaa tccaccaccc cacccgtctg ccgctgccat ttttttgccg catgattggc
    19621 aagcttagcc agcgttttgg tctgggcgat ccccacacca acagtaagat gggtacgttg
    19681 tagcaccgtt gcgcgaattt ctctgccaaa atcagtcaga tcgcgacaat tatgcacacc
    19741 tgtcagatcg cagaatgcct catcaatact gtaaatctcg acgcggggcg atagttcttc
    19801 cagcgtcgac atcacccgat tactcatgtc tgcgtaaagc tcataattgc tgctaaagca
    19861 aaccacgcca cagcgacgaa acagatcttt ttgtttgaac cagggatcgc ccattttaac
    19921 gcccagcgcc tttgcctcag cgtttcgggc gataacgcaa ccgtcattat tcgatagcac
    19981 aaccaccggt ttaccccata aatcagggcg aaacaccgtc tcacagctgg cataaaacgc
    20041 gtttacatca cagagggcaa acatcagcgc atcgccttaa cgacgtggat caccacacca
    20101 aagacatcca gcgtatcttc gctactgatg gtaatgggcg agtacgcact gttcatagga
    20161 ataagctgta ccgtcgggcg tagttgcaat tttttcaccg taaactcgcc gtcaacagca
    20221 gcgatgataa tatcaccatg gctggcggta atagcgctat cgacaatcag taaatcaccg
    20281 tcactaatcc caccatcaat catagaatca ccacttgctt tgacgaaata agtcgcgctg
    20341 ggatgctgga tcaacagttg attcagatcg atgcgctgtt caacgtaatc tgcggccggt
    20401 gaaggaaagc cacactgaac aagatcgcta aatagcggaa aagtcacaat ttcgcggaga
    20461 tccgcaggct tgataaacaa cataataatc tgcctgaagt tatactgttt ttatatacag
    20521 tagtctgttc ttgtcagcag atcaatactg attcaggcta tcaatatttg tcgctgcata
    20581 gactgctgat ttttcgttct cttatcctgt gctcacgcgg ccttctggcg acagcgtcca
    20641 tccagcagaa aggaaaaata tcacctggtt taaaatagaa tcgaagcatt cgcccagaaa
    20701 ggcattctca ttaaggtact ggatgacggc atcaagaaac tgtgttctat tgctgcgggc
    20761 gtagttgaag ggaaactgat tccagaattg aagaacaagt taaaatctgt gcagagtttc
    20821 tttaccaccc tgtctaacac ggttaaacaa gcgaataaag atatcgatgc cgccaaattg
    20881 aaattaacca ccgaaatagc cgccatcggg gagataaaaa cggaaactga aacagccaga
    20941 ttctacgttg attatgatga tttaatgctt tctttactaa aagaagcggc ccaaaaaatg
    21001 atta
//
LOCUS       DACADF010000078        20988 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.079, whole genome shotgun sequence.
ACCESSION   DACADF010000078 DACADF010000000
VERSION     DACADF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.079"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..403)
                     /locus_tag="ISB30_002788"
     CDS             complement(<1..403)
                     /locus_tag="ISB30_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP1990402.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFIPTLILVMMLIDLGAFVGTQGENKYGKDTQ"
     gene            504..1103
                     /locus_tag="ISB30_002789"
     CDS             504..1103
                     /locus_tag="ISB30_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAP1990403.1"
                     /translation="MKPGCTLFILLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            1211..1978
                     /gene="tpiA"
                     /locus_tag="ISB30_002790"
     CDS             1211..1978
                     /gene="tpiA"
                     /locus_tag="ISB30_002790"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP1990404.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(2033..2788)
                     /gene="cdh"
                     /locus_tag="ISB30_002791"
     CDS             complement(2033..2788)
                     /gene="cdh"
                     /locus_tag="ISB30_002791"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAP1990405.1"
                     /translation="MKKAGLLFLVMIVIAVVTAGIGYWKLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(2895..3884)
                     /locus_tag="ISB30_002792"
     CDS             complement(2895..3884)
                     /locus_tag="ISB30_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1990406.1"
                     /translation="MKKWGVGLTFLLVATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(4204..5166)
                     /gene="pfkA"
                     /locus_tag="ISB30_002793"
     CDS             complement(4204..5166)
                     /gene="pfkA"
                     /locus_tag="ISB30_002793"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP1990407.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(5347..6249)
                     /gene="fieF"
                     /locus_tag="ISB30_002794"
     CDS             complement(5347..6249)
                     /gene="fieF"
                     /locus_tag="ISB30_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAP1990408.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(6398..6898)
                     /gene="cpxP"
                     /locus_tag="ISB30_002795"
     CDS             complement(6398..6898)
                     /gene="cpxP"
                     /locus_tag="ISB30_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAP1990409.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7048..7746
                     /gene="cpxR"
                     /locus_tag="ISB30_002796"
     CDS             7048..7746
                     /gene="cpxR"
                     /locus_tag="ISB30_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAP1990410.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEATFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7743..9116
                     /gene="cpxA"
                     /locus_tag="ISB30_002797"
     CDS             7743..9116
                     /gene="cpxA"
                     /locus_tag="ISB30_002797"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAP1990411.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9287..9961)
                     /gene="yiiM"
                     /locus_tag="ISB30_002798"
     CDS             complement(9287..9961)
                     /gene="yiiM"
                     /locus_tag="ISB30_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAP1990412.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSVAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10110..11093)
                     /gene="kdgT"
                     /locus_tag="ISB30_002799"
     CDS             complement(10110..11093)
                     /gene="kdgT"
                     /locus_tag="ISB30_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAP1990413.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11353..11973)
                     /gene="sodA"
                     /locus_tag="ISB30_002800"
     CDS             complement(11353..11973)
                     /gene="sodA"
                     /locus_tag="ISB30_002800"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAP1990414.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQAYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPIVGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12258..13292
                     /gene="rhaT"
                     /locus_tag="ISB30_002801"
     CDS             12258..13292
                     /gene="rhaT"
                     /locus_tag="ISB30_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HAP1990415.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13289..14227)
                     /gene="rhaR"
                     /locus_tag="ISB30_002802"
     CDS             complement(13289..14227)
                     /gene="rhaR"
                     /locus_tag="ISB30_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAP1990416.1"
                     /translation="MVFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVSFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(14211..15047)
                     /gene="rhaS"
                     /locus_tag="ISB30_002803"
     CDS             complement(14211..15047)
                     /gene="rhaS"
                     /locus_tag="ISB30_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAP1990417.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHCVLQQVRQLVAQLEQQEEGNDLPSTASREILFMQLLLLLRKS
                     SLQESVENSASRLNLLLAWLEDHFADEVNWDAVAAQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15335..16804
                     /gene="rhaB"
                     /locus_tag="ISB30_002804"
     CDS             15335..16804
                     /gene="rhaB"
                     /locus_tag="ISB30_002804"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP1990418.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQHGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            16801..18060
                     /gene="rhaA"
                     /locus_tag="ISB30_002805"
     CDS             16801..18060
                     /gene="rhaA"
                     /locus_tag="ISB30_002805"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP1990419.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLLDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRTYEKKILSRRG"
     gene            18327..19151
                     /gene="rhaD"
                     /locus_tag="ISB30_002806"
     CDS             18327..19151
                     /gene="rhaD"
                     /locus_tag="ISB30_002806"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP1990420.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            19161..19475
                     /gene="rhaM"
                     /locus_tag="ISB30_002807"
     CDS             19161..19475
                     /gene="rhaM"
                     /locus_tag="ISB30_002807"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP1990421.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATIEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(19516..20910)
                     /locus_tag="ISB30_002808"
     CDS             complement(19516..20910)
                     /locus_tag="ISB30_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAP1990422.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
BASE COUNT         5221 a         5509 c         5218 g         5040 t
ORIGIN      
        1 cctgggtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca ccaagatcga
       61 taagcatcat caccagaatc agtgttggga taaaacgccc caccgcccat tgccataccc
      121 ccggcaaaat tgcccagtta cccgccagca gcatccacgc cacaatcatc agaaatgccc
      181 atgcgccgga acgcccgcga tcatgcaagc gcttaacggt tactgccgct gttggccaaa
      241 gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta cccgccagtg
      301 agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa tcacggcgtc
      361 caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt ccttgatggt
      421 tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt aatcggagcc
      481 agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttatt ctcttatgtt
      541 ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca acgaccgcgc
      601 cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt cgagaagact
      661 ttaacagcca gaatcccagc ctgccactga acgaatttcg tgccatcgac agcagtcccg
      721 acaaagccaa tctcactcgt gcagcaagta aaattaacga gaacttgtat gcttctactg
      781 cgctggagcg cggtacctta aaaatcaaaa gcattcaaat aacctggcta cccatccagg
      841 ggccagagca aaaagccgcg aaagcgaaag ctcaggcata catggcagcg gtgatccgca
      901 cactcacccc attaatgacc aaaactcaaa gccagaaaaa actgcagtcg ctactaacgg
      961 cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat gttgtcgcgg
     1021 acaacggcga aaaggggctg accttcgctg ttgaaccgat taagctggcg ctatctgaat
     1081 cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga atctctatac
     1141 tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct tataagcgtg
     1201 gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca
     1261 catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg ctggctgtgc
     1321 ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgtgaag ctgaaggcag
     1381 ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat tcaccggtga
     1441 aacctctgct gctatgctga aagacatcgg cgcacagtac atcatcatcg gccactctga
     1501 acgtcgtact taccacaaag aatctgacga actgatcgcg aaaaaattcg cggtgctgaa
     1561 agagcagggt ctaactccgg ttctgtgcat cggtgaaacc gaagcggaaa acgaagcggg
     1621 caaaactgaa gaagtttgtg cacgtcagat cgacgcggta ctgaaaaccc agggtgctgc
     1681 ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta ctggcaaatc
     1741 tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca ttgctaaagt
     1801 tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa acgcgtctaa
     1861 cgctgcagaa ctgtttgctc agccagacat cgacggcgcg ctggttggcg gtgcttctct
     1921 gaaagctgac gctttcgccg tgatcgttaa agctgcagaa gcggctaaac aggcttaagt
     1981 ctgacaggtg ccggatttca tatccggcac ttactttcct aaactcctcg ccttaacgca
     2041 aaatctcaca ctgatgatcc tggatttctt cggctgaagc acggttcagc gtcagtagat
     2101 ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc gccagcccgt
     2161 agcttcccat atgttcccgc gcctcaggca cttcttccgc cagcatcata aatgggctgc
     2221 gttgtaccag ttcgctttcc gttacccgac gcgccaggta ctcatgccca cgcaaaccac
     2281 ctggcagtgg caaccagcgg ctactgatgt tcgccagatt gttatccagc tgttcgcgca
     2341 catcaggacg aatacaagaa atatgaatat gaaaatggtt ttgcgtacgc ccggtgcggg
     2401 agttgatcgc cagagatacc gcgcgatcgg gaacaggctg gccgtatttt tggctcataa
     2461 aatcacgcgc ctgccaggct aaccagaaaa aattaggcgt tgacggatcg gtcaacaatg
     2521 ggctttcagt accgttaata cggtacgttg gcattaacag atattgcagt gggccatgac
     2581 gatcttttaa aaccacgtat ccggcgttgg gtttgacttc cgcacatggc gacgggtttt
     2641 ggttttcctg ctggttgggc aaacattgct caagaactat cttacgtaac gtgtctgact
     2701 cttcaccggt taatttccag taaccaatac cggcagtcac aacggcgata actatcatca
     2761 ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa gagatgcaag
     2821 ggtaacgcaa aatcgtgaca aataaaaaac ccagtggcga gaaaccaccg ggcttgataa
     2881 ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt agcaaaatgc
     2941 tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa cttcaatttt
     3001 ggaaacgcat tttcgtactt tttcgctacc tcagcgtcgc gcggacggta gtagtttttc
     3061 gctgcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc cgccacctct
     3121 ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc tgcgaggata
     3181 gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc tgccagcaga
     3241 gcttcgtttt cccaggcaat cagtacatcg ccaattccgc gctcgacaaa agtgttagtg
     3301 gatccacgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg aacaaaatcc
     3361 tgtgcttttg cctgatcgtt gttgttgtga tgaagcgcgt agccccaggc tgccaggtaa
     3421 ttccagcgcg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac acccggttta
     3481 atcagatcgt tccagtcatg gatctgcttc ggattaccct tacgtaccag gaaaacaatg
     3541 gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt atcaatccgc
     3601 ccgcgttccg caattgcgtc cacgtcatag gccagagcca gcgtgacaac atcagcttca
     3661 ataccgttga ttaccgacgt cgcttgtttg cctgaaccac cgtgtgactg acgaatcacc
     3721 acgttatcgc cagtttgctg tttccagtgg gcgctgaatg ccttgttgta ctgttcgtac
     3781 aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc cataacgctg
     3841 gttgccacca gcaaaaatgt taaccctacg ccccactttt tcatcgcccg actctcttat
     3901 gttgtgttgt gatgagcaaa gcgtgccaga aggttgacca aacattaaag aataaaaaaa
     3961 gattggctat aacttgcgag tatatgttgg gggattaaaa aggcggaaga aatccgcctc
     4021 atattgctga caaagtgcgc tttgtccatg ccggatgcgg cacaaacgcc ttatccggcc
     4081 tacaaaagtt tgcaaattca atagattaca gaattcatgt aggcctgata agcgaagcgc
     4141 atcaggcctt tttgcttctg tcatcggttt caggctaaag gaatctgcct ttttccgaaa
     4201 tcattaatac agttttttcg cgcagtccag ccagtcgcct ttgaacggac gcttcatgtt
     4261 ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac ctacgcaacg
     4321 accaccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg aagccagaat
     4381 acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag ttgcgcgggt
     4441 ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac acatatgttc
     4501 ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga tttcgtttac
     4561 caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc caccggcaat
     4621 ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca cggaaatacg
     4681 ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg tgctcagcgc
     4741 agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag tgcccggcag
     4801 gccgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg aaccgtcacc
     4861 gccgataacc accagcgcgt cgatcccacg ttttttcagg ttttcgatag ccacggcgcg
     4921 gatgttctcg tcgcggaatt ccgggaaacg cgcagaaccg aggaacgtac cgccacggtt
     4981 gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat acagacccag
     5041 atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac gaacaacccc
     5101 gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac cgattttctt
     5161 aatcatgact acctctgaac tttggaatgc aaaatgaaat ctgttgccgg aagtcttctt
     5221 gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc tgctgaattg
     5281 attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg ctctctttta
     5341 tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac aggggtcctg
     5401 atggataatc acatccgatc ccggaaaacg ccgtaaaata gcctgctcta cctgatccgc
     5461 caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa tctgaataaa
     5521 gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac ccggccagga
     5581 agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat ccagtaatga
     5641 ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc cgatccccaa
     5701 tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg ccagcagaat
     5761 tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca ccgcctggct
     5821 ttgcgtccgg cgcaccaccc aacgctgaaa cgagacaagg ataatcgtac aaattagcgc
     5881 cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag ataccagatg
     5941 ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac tttgcgccag
     6001 cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg caggttgcag
     6061 ggaatatcgc accaccagta aattcgtcaa cgacgcaccg atatccacca gcgaatccac
     6121 cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt taatcagcaa
     6181 tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc gtccataaga
     6241 ttgattcata aatactcccg ctatcaactg acggtagtat aacggaagca aatcatctgc
     6301 aatgcattaa gcagcaggca aattgaggat aaaaaaaacc cccacatcat gtgggggaag
     6361 acagggatgg tgtctatggc aaggaaaaca gggtttacta ctgggaacgt gagttgctac
     6421 tactcaatag cttcaacgat gaactttttt gccattgcgt cacgtcgcgc aactgctcca
     6481 ttcgttgttg atgtttctcg tttaaaaccg cttgctgctc cggcgttaac aggcgataca
     6541 tttggttgcg gacttttgcc atctcaacct gacgagcaat ttgctcattc gccatttttt
     6601 ctgcctgtgc gcgcacagcg ttttcatcaa aattttctgc ggtgacaagg cgatgcattg
     6661 tctccagttc gctaacatta acaggaggct gttcgtgccg ggcctgttgc ataagatctc
     6721 gcatctgctg acgctgatgt tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac
     6781 tgcgctgcgt aagttcttca cccggatgcc agttatcgcc tgaaccgact tcagcagcgt
     6841 ggcttaatga actgactgcc agcgttgagg ccatgacggc agcggtaact atgcgcatca
     6901 tttgctccca aaatctttct gtcgcgattc aacgatagag agtttacgat tcaggctgca
     6961 aacatgcgtc agggggtgta aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt
     7021 aatttctgcc tcggaggtat ttaaacaatg aataaaatcc tgttagttga tgatgaccga
     7081 gagctgactt ccctattaaa ggagctgctc gagatggaag gcttcaacgt gattgttgcc
     7141 cacgatgggg aacaggcgct tgatcttctg gacgacagca ttgatttact tttgcttgat
     7201 gtaatgatgc cgaagaaaaa tggtatcgac acattaaaag cacttcgcca gacacaccag
     7261 acgcctgtca ttatgttgac ggcgcgcggc agtgaacttg atcgcgttct cggccttgag
     7321 ctgggcgcag atgactatct cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt
     7381 cgcgcgatcc tgcgccgttc gcactggagc gaacaacagc aaaacaacga caacggttca
     7441 ccgacactgg aagttgatgc cttaagtctc aatcctggtc gccaggaagc caccttcgac
     7501 gggcaaacgc tggagttaac cggtacggag tttaccctgc tctatttgct ggcacagcat
     7561 ctgggtcagg tggtttcccg tgagcattta agccaggaag tgctgggcaa gcgcctgacg
     7621 cctttcgacc gcgctatcga tatgcacatt tccaacctgc gtcgtaaact gccggatcgt
     7681 aaagatggtc acccgtggtt taaaaccttg cgtggtcgcg gctatctgat ggtttctgct
     7741 tcatgatagg cagcttaacc gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg
     7801 tgttgatgtt ggttttgatg ttacccaagc tcgattcacg ccagatgacc gagcttctgg
     7861 atagcgaaca gcgtcagggt ctgatgattg agcagcatgt cgaagcggag ctggcgaacg
     7921 atccgcccaa cgatttaatg tggtggcggc gtctgttccg ggcgattgat aagtgggcac
     7981 cgccaggaca gcgtttgtta ctggtgacca ccgaaggccg cgtgatcggc gctgaacgca
     8041 gcgaaatgca gatcattcgt aactttattg gtcaggccga taacgccgat catccgcaga
     8101 agaaaaagta tggccgcgtg gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata
     8161 attaccaact ttatctgatt cgtccggcca gcagttctca atccgatttc ataaacttac
     8221 tgtttgaccg cccgctatta ctgctgattg tcaccatgct ggtcagtacg ccgctgctgt
     8281 tgtggttggc ctggagtctg gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag
     8341 ttgcccaggg aaacttacgc cagcacccgg aactggaagc ggggccacag gaattccttg
     8401 ccgccggtgc cagttttaac cagatggtca ccgcgctgga gcgcatgatg acctctcagc
     8461 agcgtctact ttctgatatc tctcacgaac tgcgcacccc gctgacgcgt ctgcaactgg
     8521 gtacggcgtt actgcgccgt cgtagcggtg aaagcaagga actggagcgt attgaaaccg
     8581 aagcgcaacg tctggacagc atgatcaacg atctgttggt gatgtcacgt aatcagcaga
     8641 aaaatgcgct ggttagcgag accatcaaag ccaaccagtt gtggagtgaa gtgctggata
     8701 acgcggcgtt cgaagccgag caaatgggca agtcgttgac ggttaacttc ccacctgggc
     8761 cgtggccgct gtacggcaac ccgaacgccc tggaaagtgc gctggaaaac attgttcgta
     8821 atgctctgcg ttattcccat acgaagattg aagtgggctt tgcggtagat aaagacggta
     8881 tcaccattac ggtggacgac gatggtcctg gcgttagccc ggaagatcgc gaacagattt
     8941 tccgtccgtt ctatcggacc gatgaagcac gcgatcgtga atctggcggt acaggtttgg
     9001 ggctggcgat tgttgaaacc gccattcagc agcatcgtgg ctgggtgaag gcagaagaca
     9061 gcccgctggg cggtttacgg ctggtgattt ggttgccgct gtataagcgg tcataacgtc
     9121 aaccttcagg taaaaaaatg cctgatgcac tacgcttatc aggcctacaa aacctgttga
     9181 atttatgggt tttgtaggct ggataaggcg tttacgccgc atccggcatt tgagcaggat
     9241 gcctgatgcg acgctgatag cgtcttatca ggcctacact ccggggttat tttccccaca
     9301 aacggcggga aaaatcttcg atcttgccgc tcagtcggcg cttttgcatc gttcgcgtcc
     9361 agcttttcga taaccctgca acggagagta aacggtgata ctgatcgtca tcaaacggca
     9421 tatgccatgc gatggcggca gcctcctgca cggtcacatc gctgacacgg gaaaccaact
     9481 ccagtggcgc atcagcagat accaaccctg gtgcaattac gctatacaac caccccactt
     9541 tacccgtgtt ttgcatcaac tgcgctatat cgctgatatc aaaatggtaa ttgagtttat
     9601 agcaaggtga gcgtggctgg ctgacctgaa ttaatgcctc tccccagcgg aaaatatcac
     9661 caatataaac attactttcc gtcagaccgt cggttgagag attttcgcca aacgcaggcg
     9721 caacaaacag ctccgcctgt tccggaaatt cccgcgccca gtagagataa tgctcacgag
     9781 gataatgaca cagcgctctg tctggcccgc cgtggatctt tttctccgcc tgttcgtcac
     9841 cttccagccc caggtccgtc agcatcaact caccatcaac ctggattttg gcaattgcgc
     9901 tgggtttgcc ttcgggataa gcctgaatct tgcctgtgta tacatcaacc ggatatcgca
     9961 tctgttccct ctccacgaga aatttcccca ttagaccata tttattttat gtcaggcgtg
    10021 ctgttttcgc ggtaagtatc caggcagaaa cgcaattgaa aaccggacga acgttagttc
    10081 atccggctgt ggggattaag gaagctggtt tatttcaccg tacctaaaat ttcgattttc
    10141 gccgctctgg ctttgacttt acgtgaccag atagaggtaa gaattggcac cagaatcgaa
    10201 gtcacaatga ccgccgtcgc taccagcgaa gttgctgccg gagccatagg tttaaacgca
    10261 ggcaccattt ctgcaatcag cacaggtgtc gctaccgcgg ccccagcgga actggaagcg
    10321 gcaattccgg cagtgccatc gccaccgcca atcaatttat cggcgataat caacggaata
    10381 ccggtcacga taattactgc cacacccaac aggatcccca gcaaacctgt ctgggcaatt
    10441 acagtcaaat caatggtatt gcccagtgcg aaggcaaaga atggaatcag cgtctgcacc
    10501 gctttgctga aaaattctcg taactcaggg tcaaggttcc ccagggcaaa gcccaccagg
    10561 aacggcaata ctgcgccgac gaaaacatgc ggttcaaacg aggcaatccc ggcagtgccc
    10621 agaataatca tcgtcatgag cggcccggac tccaacgaca tcaacacaaa tgccccagct
    10681 tcttcttttg tgccgtactg ctgcatgatg gaagcgtaaa gtccgccgtt ggtcatatcc
    10741 atcgccgcca ccagcgccaa cgttgaaagt ccggcaaaaa atccaacttc aacaccatgt
    10801 tccggaataa tgcgcgaggc aattgccgca accacccacg cgacggcaat tttagttacc
    10861 accagcgtac cggatttacg cagtaccgtt cccgtcgcgc ttaattttat tgacgccccc
    10921 atgcaaaaaa accacaccgc cagaatgggc accgtaccgg taatcatccc gttggtgaat
    10981 gatccaaaat atttccccgc ccccggcgag aaggtgtggc acagtgcgcc aaggaatagc
    11041 gggacgagca tcatcccccc cgggattttc tcaatcgagc gttttatctg catttccatc
    11101 acctgtcggc atatttttaa gggctgatgc gtcattcctc aacggagcat cagtgttgat
    11161 ggaaacgaag ataaacattc atttgatcaa taaaagtgat caaaaccaca tcaattgaaa
    11221 cgatgtttta tttttatcgg atcgttgttt ttattttgaa agtgatcaca aaaaaaccac
    11281 cgccgttggc gatggttcat tttttaagct gcaatgcggc gtaaacgcct cattgcagca
    11341 ggcgtcaaat gattattttt tcgccgcgaa acgtgccgct gcttcgtccc agttcaccac
    11401 gttccagaac tctttaatgt agtccggacg gcggttctgg aatttcaggt agtaagcatg
    11461 ttcccacaca tccaggccca caatcgggaa gccggaagcg ccagaaatgg cttcgcccat
    11521 cagcggagag tcctggttag cagtagaaac caccgccagt ttatcgcctt tttgcaccag
    11581 ccatgcccag ccggaaccaa agcgggaagc tgccgctttt tcaaattctg ctttgaaatt
    11641 atcaacggaa ccgaaatcac gttcgatagc cgctttcagg tcgccctgca gggtggtgcc
    11701 ttttttcaga cctttccaga acagactgtg gttagcgtga ccgccagcgt tattgcgcag
    11761 tacggttttc ttgtctgctg gcagctggtc cagtttggtg atcagctctt caaccggcag
    11821 gttagcgaat tctggcaggc tttccagcgc cgcattggcg ttgttaacgt aggcctgatg
    11881 gtgtttggtg tggtggattt ccatggtctg cttatcgaag tgcggttcca gggcatcgta
    11941 agcatacggc agggatggca gggtataact catattcatc tccagtgtgg tcgggcggcc
    12001 gattgttaat gccgcgtaag cagttggttc attatagtta attaaatgat attgaaaatg
    12061 attatcaatg ccgtactttt cataagggta tggttttgca ggaaaatgcc caagatgtga
    12121 agcaaatcac ccacttaatg ccgtgattgc cagtaaatcg gcagcggcgg caacaggcga
    12181 aaggttaatc gacagctcaa tttttacact catctcgtcg gagatgtgac gcgacgaaaa
    12241 atgatgagga taagaagatg agtaacgcga ttacgatggg gatattttgg catttgatcg
    12301 gcgcggccag tgcagcctgt ttttacgcgc cgttcaaaaa agtaaaaaag tggtcatggg
    12361 aaaccatgtg gtcagtcggt gggattgttt cgtggattat cctgccgtgg gccatcagcg
    12421 ccctgttact accgaatttc tgggcgtatt acagctcatt tagtctctct acgctgctgc
    12481 ctgtttttct gttcggcgct atgtggggga tcggtaatat caactacggc ctgaccatgc
    12541 gttatctcgg catgtcgatg ggaattggca tcgccattgg cattacgttg attgtcggta
    12601 cgctgatgac gccaattatc aacggcaatt tcgatgtgct gattaatacc gaaggcggac
    12661 gcatgacgtt gctcggcgtt ctggtggcgc tgattggcgt agggattgta actcgcgccg
    12721 ggcagttgaa agagcgcaag atgggcatta aagccgaaga gttcaatctg aaaaaagggc
    12781 tggtgctggc ggtgatgtgc ggcattttct ctgccgggat gtcctttgcg atgaacgccg
    12841 caaaaccgat gcatgaagcc gctgccgcgc tgggcgtcga tcccctgtat gtcgctttgc
    12901 caagctatgt tgtcatcatg ggcggcggcg caatcatcaa cctcggcttc tgcttcattc
    12961 gtctggccaa agtgaaagat ttgtcgctaa aagccgactt ctcgctggca aaaccgctga
    13021 tcattcacaa tgtgttactc tcagcactgg gcgggttgat gtggtatctg caattctttt
    13081 tctatgcctg gggccacgcc cgcattccgg cgcagtatga ctacatcagc tggatgctgc
    13141 atatgagctt ctatgtattg tgcggcggta tcgtcgggct ggtgctgaaa gagtggaaca
    13201 atgcaggacg ccgtccggta acggtgttga gcctcggttg tgtggtgatt attgtcgccg
    13261 ctaacatcgt cggcatcggc atggcgaatt aatctttctg cgaattgaga tgacgccact
    13321 ggctgggcgt catcccggtt tcccgggtaa acaccaccga aaaatagtta ctatcttcaa
    13381 agccacattc ggtcgaaata tcactgatta acaggcggct atgctggaga agatattgcg
    13441 catgacacac tctgacctgc cgcagatatt gattgatggt cattccagtc tgctggcgaa
    13501 attgctgacg caaaacacgc tcactgcatg atgcctcatc acaaaattta tccagcgcaa
    13561 agggactttt caggctagcc gccagccggg taatcagctt atccagcaat gtttcgctgg
    13621 atgttggcgg caacgaatca ctggtgtaac gatggcgatt cagcaacatc accaactgcc
    13681 cgaacagcaa ctcagccatt tcgttagcaa acgacacatg ctgactactt tcatgctcaa
    13741 gctgaccgat aacctgccgc gcctgcgcca tccccacgct acctaagcgc cagtgtggtt
    13801 gccctgcgct ggcgctaaat cccggaatcg cgccctgcca gtcaagattc agcttcagcc
    13861 gctccgggca ataaataata ttctgcaaaa ccagatcgtt aacggaagcg taggagtgtt
    13921 tatcgtcagc atgaatataa aagagatcgc cacgggtaat gcgataaggg cgatcgttga
    13981 gtacatgcag accattaccg cgccagacaa tcaccagctc acaaaaatca tgtgtatgtt
    14041 cggcaaagac atcttgcgga taacggtcag ccacagcgac tgcctgctgg tcgctggcaa
    14101 aaaaatcatc tttgagaagt tttaactgat gcgccaccgt ggctacctcg gccagagaac
    14161 gaagttgatt attcgcaata tggcgtacaa atacgttgag aagattcgcg ttattgcaga
    14221 aaaccatccc gtccctgacg aatatcacgc ggtgaccagt taaactctcg gcgaaaaagc
    14281 gtcgaaaagt ggttactgtc gctgaatccg cagcgatagg cgatgtcagt aacgctggcc
    14341 tcgctgtggc gtagcagatg tcgggctttc atcagtcgca ggcggttcag gtaacgctga
    14401 ggcgtcagtc ccgtttgctg cttaagctgc cgatgtagcg tacgcaatga aagagaaaat
    14461 tgagccgcca ctgcatccca attcacctca tcggcaaaat ggtcctccag ccaggccaga
    14521 agcaagttga gacgtgatgc gctgttttcc acgctctcct gcaaactgct tttacgcagt
    14581 aagagcagta attgcataaa cagaatttca cgactggcgg tcgagggtaa atcatttccc
    14641 tcttcttgtt gttccaactg tgcaaccagt tggcgcacct gctgtaatac gcaatggtta
    14701 acgcgccagt gagacggata ctgcccatcc tgctcttgtg gcagcaactg attcagcccg
    14761 gcgagaaact gaaatcgatc cggcgagcga tacagcacat tggtcagaca cagattatcg
    14821 gtatgttcat acagatgccg atcatgatcg cgtacgaaac aaaccgtccc accggtgatg
    14881 gtatagggct gcccattaaa tacatgaata cccgtgccat gttcgacaat cacaatttca
    14941 tgaaaatcat gatgatgttc aggaaaatcc gcctgcggga gccggggttc tatcgccacg
    15001 gacgcgttac cagacggaaa aaaatccaca ctatgtaata cggtcatact ggcctcctga
    15061 tgtcgtcaac acggcgaaat agtaatcacg aggtcaggtt cttaccttaa attttcgacg
    15121 gaaaaccacg taaaaaacgt cgatttttca agatacagcg tgaattttca ggaaatgcgg
    15181 tgagcatcac atcaccacaa tgaagcaaat tgtgaacatc atcacgttca tctttccctg
    15241 gttgccaatg gcccattttc ctgtcagtaa cgagaaggtc acgaattcag gcgcttttta
    15301 gactggtcgt aatgaaattc agcaggatca cattatgacc tttcgcaatt gtgtcgccgt
    15361 cgatctcggc gcatccagtg ggcgcgtgat gctggcgcgt tacgagcgtg aatgccgcag
    15421 cctgacgctg cgcgaaatcc atcgttttaa caatggcctg catagccaga acggttatgt
    15481 cacctggaat gtggatagcc tggaaagtgc cattcgcctt ggattaaaca aggtgtgcga
    15541 ggaagggatt cgtatcgata gcattgggat tgatacctgg ggcgtggact ttgtgctgct
    15601 cgaccagcac ggtcagcgcg tgggtctgcc cgttgcttac cgcgatagcc gcaccaatgg
    15661 cctaatggcg caggcacaac agcaactcgg caaacgcgat atttatcaac gtagcggcat
    15721 ccagtttttg cccttcaata cgatctatca gctacgtgca ctgacagagc agcaaccaga
    15781 acttattcca cacattgctc acgctttgct gataccggat tactttagtt atcgcctgac
    15841 cggcaagatg aactgggaat ataccaacgc cacgaccacg caactggtca atatcaatag
    15901 cgacgactgg gacgagtcgc tactggcgtg gagcggtgcc aacaaagcct ggtttggtcg
    15961 cccgacgcat ccgggtaatg tcataggtca ctggatttgc ccgcagggta atgagattcc
    16021 ggtggtcgcc gttgccagcc atgataccgc cagcgcggtt atcgcctcgc cgttaaacgg
    16081 ttcacgcgcc gcttatctct cttctggcac ctggtcattg atgggcttcg aaagccagac
    16141 gccatttacc aatgacacgg cgctggcagc caatatcacc aacgaaggcg gggcggaagg
    16201 tcgctatcgg gtgctgaaaa atattatggg cttatggctg cttcagcgag tgcttcagga
    16261 gcggcaaatc aacgatcttc cggcgcttat cgccgcaaca caggcacttc cggcctgccg
    16321 cttcatcatc aatcccaatg acgatcgctt tattaatcct gacgagatgt gcagcgaaat
    16381 tcaggctgcg tgtcgggaaa cggcgcaacc gatcccggaa agtgatgctg aactggcgcg
    16441 ctgtattttc gacagtctgg cgctgctgta tgccgatgtg ttgcatgagc tggcgcagct
    16501 gcgtggtgaa gatttctcgc aactgcatat tgtcggcggc ggctgccaga acacgctgct
    16561 caaccagctg tgtgccgatg cctgcggtat tcgggtgatc gccgggcctg ttgaagcctc
    16621 gacgctcggc aatatcggca tccagttaat gacgctggat gaactcaaca atgtggatga
    16681 tttccgtcag gtcgtcagca ctaccgcgaa tctgaccacc tttaccccta atcctgacag
    16741 tgaaattgcc cactatgtgg cgcagattca ctctacacga cagacaaagg agctttgcgc
    16801 atgaccactc aactggaaca ggcctgggag cttgcgaaac agcgtttcgc ggcagtaggg
    16861 attgatgtcg aggaggcact gcgccaactt gatcgtttac ccgtttcaat gcactgctgg
    16921 cagggcgatg atgtttccgg ttttgaaaac ccggaaggtt cgctgaccgg tggtattcag
    16981 gctactggta attatccggg caaagcgcgt aatgccagcg agctgcgtgc cgatctggaa
    17041 caggctatgc ggctgattcc ggggccaaaa cggcttaatt tgcatgccat ttatctggag
    17101 tcagacacgc cagtctcacg tgaccaaatc aaaccagagc acttcaaaaa ctgggttgaa
    17161 tgggcgaaag ccaatcagct cggtctggat tttaacccct cctgtttttc acatccgcta
    17221 agcgccgatg gctttacact ttcccatgcc gacgaccgca ttcgccagtt ctggattgac
    17281 cactgtaaag ccagccgccg tgtttcagcc tattttggtg agcaactcgg cacaccgtcg
    17341 gtgatgaaca tctggatccc ggatggcatg aaagatatca ccgttgaccg tctcgccccg
    17401 cgtcagcgtt tgctggcagc actggatgaa gtgatcagcg agaagctaaa ccctgcgcac
    17461 catatcgacg ccgttgagag caaattgttt ggcattggcg cggagagcta cacggttggc
    17521 tccaatgagt tttacctggg gtatgccacc agccgccaga cggcgctgtg cctggacgcc
    17581 gggcatttcc acccgactga agtgatttcc gacaagattt ccgccgccat gctgtatgtg
    17641 ccgcagttgc tgctgcacgt cagccgtccg gttcgctggg acagcgatca cgtagtgctg
    17701 ctggatgatg aaacccaggc gattgccagt gagattgttc gtcacgatct gctcgaccgg
    17761 gtgcatatcg gcctcgactt cttcgatgcc tctatcaacc gcattgccgc gtgggtcatt
    17821 ggtacacgca atatgaaaaa agccctgctg cgtgcgttgc tggaacctac cgctgagcta
    17881 cgcaagctgg aagcggcggg cgattacact gcgcgtctgg cgctactgga agagcagaaa
    17941 tcgttgccgt ggcaggcggt atgggaaatg tattgtcagc gtcacgatac gccagcgggt
    18001 agcgaatggc tggagaacgt gcggacttat gagaaaaaaa ttttgagtcg ccgcgggtaa
    18061 acactgccgg atgcggcaca agtgccttat ccggcctacg ggtcggcaat agttgtaggc
    18121 ctgataagac gcgacagcgt cgcatcaggc attgattgcc ggatgcggca cgagtgcctt
    18181 atccggccta cgggtcagca acagttgtag gcctgataag acgcgacagc gtcgcatcag
    18241 gcattgattg ccggatgtgg cgcgagtgcc ttatccggcc tacaggtcgt gcatccgaca
    18301 acaccgaatt aacaggaaca cagaacatgc aaaacataac ccagtcctgg tttgtccagg
    18361 gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc aacggcggca
    18421 acctgacgct gcgcctggat gacgccgata tcgcaccata tcacgacaat tttcaccaac
    18481 aaccgcgcta tatcccgctc agccagccca tgcctttact ggcaaataca ccgtttattg
    18541 tcaccggctc cggcaaattc ttccgtaacg tccagcttga tcctgcggct aacttaggca
    18601 tcgtaaaagt cgacagcgac ggcgcgggct accacattct ttgggggtta accaacgaag
    18661 ccgttcccac ttctgaactt ccggctcact ttctctccca ctgcgagcgc attaaagcca
    18721 ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc gccctcacct
    18781 atgtacttga aaacgacacc gcggtcttca ctcgccaact gtgggaaggc agcaccgagt
    18841 gtctggtggt attcccggat ggcgttggca ttctgccgtg gatggtgccc ggcacggacg
    18901 aaatcggcca ggcaactgca caagagatgc aaaaacattc gctggtgttg tggcccttcc
    18961 acggtgtgtt cggcagcggg ccgacgctgg atgaaacctt cggtttaatc gacaccgcag
    19021 aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa cagaccatca
    19081 gccgtgaaga gttgatagcg ctcggccagc gtttcggcgt tacaccactc gccagtgcgc
    19141 tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta aaccccgacg
    19201 cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa gcagtgctga
    19261 aatctcacgg tgcgcataac tacgccatct atctcgacaa agcgcgtaat ctgctatttg
    19321 ccacgataga gattgaatct gaagagcggt ggaatgcggt tgccagtaca gatgtttgcc
    19381 aacgttggtg gaaatatatg accgacgtta tgcccgctaa tgcggataac agcccagtca
    19441 gtagcgaact gcaagaagtg ttttacctgc cttaatgagt tgcggtgccg gaaaatatcc
    19501 agcaccgccc tttcatcaga agaagtattt aaagcgaacg cgtgcgccgt aacggtcatc
    19561 actatcgtcg ccgtgtatct tatcgccgtc gctatccaga atggagtaat acgtgccgag
    19621 gtaaatatta aagttctcca tatccatcac attagcgaac tgataagacg catgaatggt
    19681 gtgaatgttg tatgttcctt catcatcaat ccagcaatcg ttatcacaaa ccacgccact
    19741 aaattcatca atcttattat gtgcgtagat ataacccagc tcgaaacgtt tccacagcgc
    19801 gttaatccca gcggtgaaat ctttctcatt attagcatct aaataggcgg tattaagatt
    19861 aaccacgatg ccattttccg gatcggtttt caggccattc caggtcatgc tcatgccata
    19921 accggtacga tcggactgat cgacaaaatt acccttgcta tcggtataac cataagcatt
    19981 atttaccacg ttcgcttcca tcgctgcgga aacggtgaat tcttccgtcg gcgaccaggc
    20041 aattaccgga cgcagataag caacattttt ctgctgttcc atatcgcgtc catgataatt
    20101 accgtcgtta tataaagatg ttccgtcttc cagtaacgtg tttaattcaa aataccagtt
    20161 atcgagttgt ttgctgacga ggaaattacc gccagcgtta gaacgcccgc ggccctcttt
    20221 catcatatag atataaccgc tgccatcgtc ataaagatcg ttcgcagtat taccggaatg
    20281 ttcaacaaag gtatcctgat tcagcgggaa catatcgtag gcttcaaaac ggcctacttt
    20341 gaccttccag tcgttttctt tgccaaagaa gaaaaccgca tcatcgatat ttactgaacc
    20401 gtgcatatcc cccagcggtt gtgcggagaa cccagcgaaa tagccattat ccagttttcg
    20461 cataccatca aaacctaaca gaatacgacc attgagattc cactgttcat tagtgggatc
    20521 gctattcaca tcagcgttgg tcattgccag caggccatga ttactttccg catccatatt
    20581 aaattctacg tcaccgtaga tttttaaatc gccataccct gaaagcagga gattaggcgg
    20641 ggtggcgttt ttcttctctg actccgtcgt tgattgaccg tttaccggcg tgttgccctg
    20701 tgcggtttta atttcgcgga tctcggcggc ttgttgtgtc tgaagctgtt taacctgccc
    20761 ctctgctttt gctgcccgca tttctgccgc ttccaggcgt tcttccagca atgccatgcg
    20821 ttgttccagc gttaaatgct gtgtttttgc cataccggta ctacatgcca ataataaacc
    20881 gatggctaaa gataatgtcg attttttcat tatttacgtt ccctgaaata aaggagaccc
    20941 tacgctttcc aaaaggaata cgtatataga gaaatataag gtgtgcac
//
LOCUS       DACADF010000079        20862 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.080, whole genome shotgun sequence.
ACCESSION   DACADF010000079 DACADF010000000
VERSION     DACADF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.080"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            279..845
                     /gene="yqaB"
                     /locus_tag="ISB30_002809"
     CDS             279..845
                     /gene="yqaB"
                     /locus_tag="ISB30_002809"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAP1990423.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            842..1270
                     /locus_tag="ISB30_002810"
     CDS             842..1270
                     /locus_tag="ISB30_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP1990424.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1343..2899
                     /gene="gshA"
                     /locus_tag="ISB30_002811"
     CDS             1343..2899
                     /gene="gshA"
                     /locus_tag="ISB30_002811"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAP1990425.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            3049..3564
                     /gene="luxS"
                     /locus_tag="ISB30_002812"
     CDS             3049..3564
                     /gene="luxS"
                     /locus_tag="ISB30_002812"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP1990426.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3615..4727)
                     /locus_tag="ISB30_002813"
     CDS             complement(3615..4727)
                     /locus_tag="ISB30_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP1990427.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALITLPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHGLPLWLLERPEPERLLLTAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYISEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWNQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(4724..5431)
                     /locus_tag="ISB30_002814"
     CDS             complement(4724..5431)
                     /locus_tag="ISB30_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HAP1990428.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINTDYWQDLQTITQLVHTEK
                     LDGENNCLNRYGVFARSHATPTQSAWTYKIRQRWQLLKNDLGNLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGTFDPWDTQTCQPCTLEGIVSRNHDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFVYGEQS"
     gene            complement(5689..7227)
                     /gene="emrB"
                     /locus_tag="ISB30_002815"
     CDS             complement(5689..7227)
                     /gene="emrB"
                     /locus_tag="ISB30_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAP1990429.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(7244..8416)
                     /gene="emrA"
                     /locus_tag="ISB30_002816"
     CDS             complement(7244..8416)
                     /gene="emrA"
                     /locus_tag="ISB30_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HAP1990430.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(8543..9073)
                     /gene="emrR"
                     /locus_tag="ISB30_002817"
     CDS             complement(8543..9073)
                     /gene="emrR"
                     /locus_tag="ISB30_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAP1990431.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQGGVVLEAMS"
     gene            9044..9145
                     /locus_tag="ISB30_002818"
     CDS             9044..9145
                     /locus_tag="ISB30_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990432.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            complement(9164..9499)
                     /gene="ygaH"
                     /locus_tag="ISB30_002819"
     CDS             complement(9164..9499)
                     /gene="ygaH"
                     /locus_tag="ISB30_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAP1990433.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(9489..10226)
                     /locus_tag="ISB30_002820"
     CDS             complement(9489..10226)
                     /locus_tag="ISB30_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP1990434.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(10350..11534)
                     /locus_tag="ISB30_002821"
     CDS             complement(10350..11534)
                     /locus_tag="ISB30_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1990435.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGSWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(11726..12718)
                     /gene="proX"
                     /locus_tag="ISB30_002822"
     CDS             complement(11726..12718)
                     /gene="proX"
                     /locus_tag="ISB30_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAP1990436.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNANTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEDDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(12775..13839)
                     /gene="proW"
                     /locus_tag="ISB30_002823"
     CDS             complement(12775..13839)
                     /gene="proW"
                     /locus_tag="ISB30_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAP1990437.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPAAAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPSQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(13832..15034)
                     /gene="proV"
                     /locus_tag="ISB30_002824"
     CDS             complement(13832..15034)
                     /gene="proV"
                     /locus_tag="ISB30_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAP1990438.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDVELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(15390..16349)
                     /gene="nrdF"
                     /locus_tag="ISB30_002825"
     CDS             complement(15390..16349)
                     /gene="nrdF"
                     /locus_tag="ISB30_002825"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP1990439.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(16359..18503)
                     /gene="nrdE"
                     /locus_tag="ISB30_002826"
     CDS             complement(16359..18503)
                     /gene="nrdE"
                     /locus_tag="ISB30_002826"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP1990440.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISERYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(18476..18886)
                     /gene="nrdI"
                     /locus_tag="ISB30_002827"
     CDS             complement(18476..18886)
                     /gene="nrdI"
                     /locus_tag="ISB30_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP1990441.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(18883..19128)
                     /gene="nrdH"
                     /locus_tag="ISB30_002828"
     CDS             complement(18883..19128)
                     /gene="nrdH"
                     /locus_tag="ISB30_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP1990442.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(19375..19704)
                     /locus_tag="ISB30_002829"
     CDS             complement(19375..19704)
                     /locus_tag="ISB30_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAP1990443.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            19856..20200
                     /locus_tag="ISB30_002830"
     CDS             19856..20200
                     /locus_tag="ISB30_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAP1990444.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(20237..20686)
                     /gene="alaE"
                     /locus_tag="ISB30_002831"
     CDS             complement(20237..20686)
                     /gene="alaE"
                     /locus_tag="ISB30_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAP1990445.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         5170 a         5303 c         5446 g         4943 t
ORIGIN      
        1 ttctccgtaa ccttcagcaa tgaaggtatc gaagaggtag cggtattaac cgcaggcacc
       61 agggaggata acgttgcgcc agcagcggcc cgaagggcga acgccagtga gtcatcctcc
      121 cggatgcacc atattatcca aaaaatttcc ttcaaaaata tctcatttct aaataactac
      181 tttatccatt tcctgaattt tcttctcctt ctccatcagc gacaaaatca agcaattacg
      241 ctaaagtagc gtcatatttt tcgacttgcg agaagaccat gtacgagcgt tatgcaggtt
      301 taatttttga tatggatggc accatcctgg ataccgagcc tacgcaccgt aaagcgtggc
      361 gcgaagtatt agggcactac ggtcttcagt acgatgttca ggcgatgatt gcgcttaatg
      421 gctcgcccac ctggcgtatt gctcaggcga ttattgagct gaatcaggcc gatctcgacc
      481 cgcatgcgtt agcgcgtgaa aaaacagaag cagtaagaag tatgctgctg gatagtgtcg
      541 agccgcttcc tcttgttgaa gtggtgaaaa gctggcatgg tcgtcgccca atggctgtag
      601 gaacggggag tgaaagcgcc atcgctgagg cattgctggc gcacctggga ttacgccgtt
      661 attttgacgc cgtcgtcgct gccgatcacg tcaaacacca taaacccgcg ccagacacat
      721 ttttgttgtg cgcgcagcgt atgggcgtgc aaccgacgca gtgtgtggtc tttgaagatg
      781 ccgatttcgg tattcaggcg gcccgtgcag caggcatgga cgccgtggat gttcgcttgc
      841 tgtgagtgaa gcgttatcgc ttttctcgtt gtttgccagt agttttctca gcgctacact
      901 attacccggc aactccgaag tcgtgctggt ggcaatgttg ctttccggag tgagtcatcc
      961 ctgggtttta gttttaacag caacaatggg taatagcctt ggagggttaa ctaacgttat
     1021 ccttgggcgt ttctttccat tgcgtaaaac atcgcgctgg caagagaaag ctaccggctg
     1081 gctgaaacgc tatggtgcag tcacgctatt attaagctgg atgcccgtgg ttggcgattt
     1141 actgtgtctg ttagcgggat ggatgcgcat ctcgtgggga ccggtaatct tttttttgtg
     1201 ccttggtaaa gcgttacgct atgttgcagt tgcagcagct accgttcagg gcatgatgtg
     1261 gtggcactaa ttgtaggcct gcacatatgg tcaccattac agttatgcta attaaaacga
     1321 ttttgacagg cgggaggtca atttgatccc ggacgtatca caggcgctgg cctggctgga
     1381 aaaacatcct caggcgttaa aggggataca gcgtgggctg gagcgcgaaa ctttgcgtgt
     1441 taatgctgat ggcacactgg caacaacagg tcatcctgaa gcattaggtt ctgcactgac
     1501 ccataaatgg attactaccg attttgcgga agcattgctg gaatttatta caccagtgga
     1561 tggtgatatt gaacatatgc tgacctttat gcgcgatctg catcgctata cggcgcgcaa
     1621 tatgggcgat gagcggatgt ggccgttaag tatgccatgc tacatcgcag aaggtcagga
     1681 catcgaactg gcacagtacg gcacttctaa caccggtcgc tttaaaacgc tgtaccgtga
     1741 agggctgaaa aatcgctacg gcgcgctgat gcaaaccatt tccggcgtgc actataattt
     1801 ctctttgcca atggcattct ggcaggcgaa gtgcggtgat atctcgggcg ctgatgccaa
     1861 agagaaaatt tctgcgggtt atttccgcgt tatccgtaac tactatcgct tcggttgggt
     1921 cattccttat ctgtttggtg cgtctccagc gatttgttct tctttcttgc aaggaaaacc
     1981 aacgtcgctg ccgtttgaga aaaccgagtg cggtatgtat tacctgccgt atgcgacctc
     2041 tcttcgtttg agcgatctcg gctataccaa taaatcgcaa agcaatcttg gtatcacctt
     2101 caatgatctc tacgagtacg tggcgggcct taaacaggca atcaaaacgc catcggaaga
     2161 gtacgcgaag attggtattg agaaagacgg taagcggctg caaatcaaca gcaatgtgct
     2221 gcagattgaa aacgaactgt acgcgccgat tcgtccaaaa cgcgttaccc gcagtggtga
     2281 gtcgccttct gatgcgctgt tacgtggcgg cattgaatat attgaagtcc gctcgctgga
     2341 catcaacccg ttctcgccga ttggtgtaga tgaacaacag gtgcgattcc tcgacctgtt
     2401 tatggtctgg tgtgcgctgg ctgacgcacc ggaaatgagc agtagcgaac ttgcctgtac
     2461 acgcgttaac tggaaccggg tgatcctcga aggtcgcaaa ccgggcctga cgctgggtat
     2521 cggctgcgaa accgcgcagt tcccgttacc gcaggtgggt aaagatctgt tccgcgatct
     2581 gaaacgcgtc gcgcaaacgc tggatagcat taacggcggc gaagcgtatc agaaagtgtg
     2641 tgatgaactg gtcgcctgct tcgataatcc cgatctgact ttctctgccc gtatcttaag
     2701 gtctatgatt gatactggta ttggcggaac aggcaaagcg tttgctgaag cgtaccgtaa
     2761 tctgctgcgt gaagagccgc tggaaattct gcgcgaagag gattttgtcg ccgagcgcga
     2821 ggcgtctgaa cgccgtcagc aggaaatgga agccgctgat accgaaccgt ttgcggtgtg
     2881 gctggaaaag cacgcctgac agaaaagaaa aaggccactc gtgagtggcc aaaatttcat
     2941 ctctgaattc agggatgatg ataacaaatg cgcgtctttc atatactcag actcgcctgg
     3001 gaacaaagag ttcagaaaat tttaaaaaaa attaccggag gtggctaaat gccgttgtta
     3061 gatagcttca cagtcgatca tacccggatg gaagcgcctg cagttcgggt ggcgaaaaca
     3121 atgaacaccc cgcatggcga cgcaatcacc gtgttcgatc tgcgcttctg cgtgccgaac
     3181 aaagaagtga tgccagaaag agggatccat accctggagc acctgtttgc tggttttatg
     3241 cgtaaccatc ttaacggtaa tggcgtagag attatcgata tctcgccaat gggctgccgc
     3301 accggttttt atatgagtct gattggtacg ccagatgagc agcgtgtcgc tgatgcctgg
     3361 aaagcggcaa tggaagacgt gctgaaagtg caggatcaga atcagatccc ggaactgaac
     3421 gtctaccagt gtggcactta ccagatgcac tcgttgcagg aagcgcagga tattgcgcgt
     3481 aacattctgg aacgtgacgt gcgcatcaac agcaacgaag aactggcgct gccgaaagag
     3541 aagttgcagg aactgcacat ttagtcagtt taagcagaag aggccgggct ggtgaaaaca
     3601 ccgcccggct tttgttacct gtcagtcatc cgatattcca cgctatgtgc ttcatcaggt
     3661 tgaggaactt ccagccttga ggccatccgc atcacgaccg cttcaggaac tgcatactcg
     3721 cgtctggcat tttgctggtt ccattgcgcc catggcactt ccagataaac gatttttact
     3781 cgtgcgccgt aagccgtaaa cagactgatg agctggctgc gcagctggcg ggtaatgttc
     3841 gttgcattcc agatgaacgg ctttttctgg cgtaaaaaaa cgcgcgcctc ttcttttgcc
     3901 tgttgcacaa tacgcccggt ggcggttttg tcgtccggac tggcattgat tcgccgccgc
     3961 atatcatcaa ggctgataac gtccattccc tggcactgtt cactgatata gcgatctttg
     4021 cccatacccg gtaagccaca cagcaaaata acctcaaaag gttcagcctc ccagggaaca
     4081 aaatcaggag aactttgctc atgagtcagg tagtgccagc gtgcggaatc agaaacgaac
     4141 gggcgcattt ttccccagca ttgttgctca tggcaaaaca gctcgaacag atcgatgcgt
     4201 tccagcattg attgctgatc cgggctttgg cgaccaagca gatcggcacg agcaagcaaa
     4261 gcgagcagac gcgtgtcgat gcgcattgca gccgtgagca acaaacgctc cggttccgga
     4321 cgttccagta accatagcgg taacccatgc agacgcacca gtgcaacaat ttgctcgcgc
     4381 agcacgaacg gcgttgggat atcgcgccat aaaatttgtc gagccgtcag ttcgccccga
     4441 cgagcatgac caggagactg aatacgtccg ttttcctgca cggtagtgct gcgcttttca
     4501 acatcatgca ataacgccgc cgcccataaa acttcctgct gttgagcggg gagttgctgg
     4561 aattctggca gagtaatgag ggcgttaagc accatttcgg tatgtacgcc gacgtcgcct
     4621 tcaccatgat gctcagggtc ttgtggcgtg tgatgcatct cctcaaccca gctaaaacgc
     4681 tgacgtagcg ccgaccagcg tttatcgtcg gtaagttgcc aaatcatgac tgttctccat
     4741 aaacgaactc atgggccatc cgcgcccgtt gccagtggcg tttccagtgc acagtggttt
     4801 tgacatgatt tttgcgcaca tacttgaaga cgttatgcga aaagtcagcg acagaaaacg
     4861 catcatgatt tcggctgaca atgccttcca gcgtacaggg ttgacaggtt tgcgtatccc
     4921 agggatcgaa tgtgccgcgc gcattggtca gcgccagaaa atcacgttgc cagctttttt
     4981 catcattccc cggctgcggg ccggagattt cgggaacgca ggggaaatca aacagagcgg
     5041 cgtaaaactg cacctcttcc cagctcagcc acatatcctg gcagcgaacg gcaaacaggt
     5101 aaaagtcctg ttccagcgcg cggtattcaa tggaatgaac ggcgtagaga ttttcaccaa
     5161 acaactccag gttgccgaga tcgtttttca gcagttgcca gcgttggcga attttatagg
     5221 tccatgccga ctgcgtaggc gtggcatggg agcgggcaaa cacgccgtag cggttaaggc
     5281 agttattttc gccatcgagt ttttctgtgt gcaccaattg cgtgatggtc tgtaaatcct
     5341 gccagtaatc ggtgttaatg cggtcatcgc tggtggtgcc tggagagaag gggtaatgcc
     5401 aggttctccc gtatttacgt tgggtattca taataatcca ttattttgtg tgtaataaat
     5461 gtacgcccgc gagggtgcga gttttggggt taaaagaaaa atgtgagtat cacccgcgaa
     5521 agcgagggcg aaggaaaaag agaattaaca acctgagcga atgctcagaa ccggctaatg
     5581 gtgaatggaa gagtttgggg cggcgaaaag atacaccgaa tgagtgtata aaacaaccgt
     5641 ttgcagaatg agcaaataaa aagggagccg cagctccctt tggcaacctt agtgcgcacc
     5701 gcctccgccg ccacctgcgc caaatggcgg tttagcaaac cacaccagcc ccagcaggac
     5761 gaggaatatc ccggctgaca tccagaagat ctcattggcg gaaataatca gcccctgatt
     5821 ggttatctgc tgggcaatcc agcctgatgc ctgctgttgc gtcataccaa gcccttccag
     5881 ttgactgtac atcgcctgag cattcgggtt gaacgggttt accgactcag tcaactgtgc
     5941 atggtgcagc gactcgcggt tggtccacat ggtcgtggtt atcgacgtgc cgatagaccc
     6001 cgccagcgtt cgcgtaaagt tggagaggct cgatgccgcc gccagtcgtt ccggtggcaa
     6061 accggacagc gtaatggtgg tcagcggcat aaagaagcag gccaccgcaa aaccctggat
     6121 aaactgtggc caggccgacg cgccaaaatc catacccggt tcaaaggtat aggcacgcca
     6181 gtagaagcag acggcataca taataaagct gaaggttacc agtcggcgca tatccagttt
     6241 atgcgcgaag cggccgataa tcggcgacag gatcaccgga ataatcccta ccggcgcaga
     6301 ggccagccct gcccacgtcg ccgtgtaacc gtagacctcc tgcaacaact gcggcagcag
     6361 aacaatcgcg ccgaagtaga gcatatacgc gaggctgata cacaagcagc cgatggtgaa
     6421 gttgcgcgac ttaaacaacg acagatcgac tatcgggtta tcgtcggtca gctcccagac
     6481 aatcaggaag cagatagcca ccaccgccac cacggtgagg atgataattt cctgtgatga
     6541 gaaccagtcc agctctttac cgcggtcgag cataatctgc aggctgccga taccaataac
     6601 cagcagtgcc agccccacgg catcaatccg ccgccgttcg gtgcgggttt cacgtccgcg
     6661 cagagtttgc agtgtcatca acaccaccgc cacgccaatc ggcacgttga tgaagaatat
     6721 ccagccccag tggtaattat cactgatata accgccgagg atcgggccgc aaattggcgc
     6781 aacaatcacc gtcatcgacc acaacgccag cgcgatcgag cgtttggctg gcgggtagtt
     6841 attcagcaat agactttgcg aaagcggaat taacggcccg gcgacaatcc cctgaatcac
     6901 gcggaagaag atcagcatat tcaggctgct ggagacacca cacgcccacg acgcaatagc
     6961 aaaggcgatg gtggaccaga ggaacagttt cacttccccg acgcgctttg ccagccagcc
     7021 ggtaagcggg atcgagatgg cattcgccac cccgaaagaa gtgattaccc acgttccctg
     7081 gctgagcgat gagcccagat tcccggcgat agtggggatc gccacgttag caatggtgga
     7141 gtccagcacc tgcatgaatg tcgccagtga cagcgcaatc gtcataatga ccagttgtgc
     7201 gccttccagc ggtttttgct gttgcatcac acgcacctct ggattagcca gcgttagctt
     7261 ttacgatatc gtcgatcagt ttattgacag gtgccaggct gatttcacgc gcggtgctta
     7321 ccgcgaccgg tgtggaacgt actttgtttg ccagtacctg accgtcacgg ttagtggtat
     7381 tgacactcac cagcgtggat aaaccgatac gcagcgggta ttgctccagc tgtttctggt
     7441 ccagttcaat acgcacaggc agacgctgaa cgactttgat ccagttaccg gtggcgtttt
     7501 gcgccggaag cagtgagaac gcgctacctg tgcccatatc cagaccaacc actttaccgg
     7561 tgtatttcac atcatcgccg taaatatcgg tggtgatggt gaccggttga ccgatacgca
     7621 tattggcaat ctgcgtctct ttaaagttgg catccaccca catattggtg gctggaacga
     7681 ccgccatcag cggcgtcgtt gggctaattt gcgccccagg ctgtaccgcg cggcgggaga
     7741 cataaccggt catcggactg acaatacggg tacgctccag cgccagccag gcgttacgta
     7801 cttcggtggc agcctgttgt acggcaggct gatcttccag tttcgtcccc agaatcatcg
     7861 cctgattggc attgtattgt tgaatagcga cgtccagttg cgcctgggca ctggtgacgg
     7921 cgtcgcgggc gtgttgcagc tcttcgcgac caatcaggtt ggcattgccc agcggcacac
     7981 ggcggttgta gtcgctttgt gctttcgcga gggcgatttt ttgcacctca atattcgcct
     8041 gcaactgttt gctgttaatc atcagctggt gggtttggcg aacgctggaa gccagtgcag
     8101 ttttggcttt ttcaaacgcc tggcgagcat ctgtcgggtc gagagtgacc agcacgtcgc
     8161 cttcttttac aaaatcggtg ttatcggccc agactttcgt cacgctgcca gacacctgag
     8221 acataatttg aatctgattc cctgccacgt atgcgtcatc ggtttcttcg aaatgacgca
     8281 gtaccaaaaa ccaataaatc cctatcgcta cggcaataat tataaagagc aaggtgagaa
     8341 ggaggagcag acgcttacgt ttgccgctct tctttaccgg ttgctgcggg gtttgagtct
     8401 ccgcatttgc gctcatattg ttctccacga tcttcttaac tcatcggctg agccgacctg
     8461 cttgccaaaa aggccagcat gttgcgatgc tggccggtta ttttttcttt tataaatctg
     8521 gatttttgag cgagatgacg cgttagctca tcgcttcgag aaccacaccg ccctgttcca
     8581 tctggtcgag acgggagagc aatttgcggg tgatttgctc gagctggtct ttttctgttg
     8641 tgctgagcgc ggaccagagt tgatgcaggc agttatgctg cggcggtaaa acctcgcgca
     8701 aaaactcgtg acctttttcc gttaattgca gatgcaggca gcggcgatcg ttatcgcttt
     8761 cacgacgttc gatccaaccg cgtttttcca gttcatcggc aatacgcgtc gcgttggtac
     8821 gggatgatcc aagagcacaa ctcaattcag aaggctgaat actgtggttt tcctgagact
     8881 ccagcgtaat caacgccata aacaacgtct cgttaatccc ctgagccttc agcattttat
     8941 tgcggttttc cagcagcttg ctttgcatat gcatgcaaag acgggtcaga aggatctcct
     9001 gataaggaaa atcttcgtgg cggctggcgc gaaattttag catttgttca atgggcgtaa
     9061 acgaactatc catttgggta tgacctcatt aatttcagcc gatatagtaa cgacagtgac
     9121 aaataaagta aatgtattgt tttagaaaaa tgattcttgt ggattatata atcgccatca
     9181 ctttccaggc gagcccatag gccagcgcac taagcagtgt tgggatgata atgctgcgtg
     9241 ttttatagaa actggcaccc agtaccgcga agccgaccag tgtgggcacg aaacggcgtg
     9301 tatcgtgcat cacttctggt gcggtagaga caaccagcag agcgcatatc gaggcgatgc
     9361 caatggtgtc gagcaaaata cctaccgcgc cacgtttggt tgggcgggca ttacccacac
     9421 gcaggcgcag cggcagatag cggaagcaat aattcgccgc gccaaccagt aacccaagca
     9481 gcagaacctc atagctcatc gggcgctcct tgccagaatg cctggattaa cgcagtgagg
     9541 cagccacaga caatgcctgc cagaatggcg acgggaatag aaaatagcgt tacgcctgca
     9601 agggcaccaa ctaacgctgc ggtaacgcaa agagattgtt tgcgctggaa agaggcgagc
     9661 aggaaactca taaagagtgc cggaagcata aaacctaatg ctgcttcaac ggcgggataa
     9721 ccttgcagca agccgctgcc ggagaatgcc cctattaccg taccaaatac ccacgatgac
     9781 catgaactga aggcaatgcc gatcatccag ttttcgctcc agcggcgatt attgcgtacc
     9841 agttttgcgg ttgcggcggc aaaaacctca tccgtcaggc caaacgccca cagggcggtt
     9901 ttcgattttt gcagacgctg aataatacgg ctacgcagtg acgggccata caaaacatgg
     9961 cgaacatcca ttgccatgac ggtcagtgca gcaacccaca aactactccc ggctgccagc
    10021 atcgcggtaa tgacgaactg gctcgcgcct gcataaatga tgcaggagaa aaaaacgctt
    10081 tcgagaggag agaatcccag acgggtcgca ttcagaccga acgcaaaggc caccggaata
    10141 taactaataa caatcggtaa actgtctttg catccttcca tgaaggtcgc cgaaccagga
    10201 gcaggctgtg gagtagggct ttccatagag tgtacgctta acgattgtta atattataaa
    10261 tagactgaat gaatatctta accttatcag actgatgggc ttcttaacac ccttataagt
    10321 gtaaagccac gaaaacggtt gctgattgct taatttgcgg cttcctgacg atgaaaacct
    10381 cgccaccaga ccagtaagtt aaccagggca atcgtcgcgc cagccagaca cacgccagcc
    10441 caactgccat gttgccaggc tgaggctgaa attagcgaac cagcggcacc gccaataaag
    10501 tagctggtca tgtaacctgc ggtcaggcga ttacgcgcat cagggtgtat tcgataaatt
    10561 accgtctggt tagtgatatg cacgccctgc acggtgaggt ccagcaccag gatgccgata
    10621 atcagcgcca gtacggaagt gtgaccaaac cagattgcca gccatgaaag taacagcaga
    10681 agcagaccga aagttgttgt gtggtgcgat ttgcccttgt cggcaaaacc gcccgccgga
    10741 cgagcgccca acgctccggc agctcccgca agtccaaaca gaccaattac accatcgctg
    10801 tagttaaaag gtggagcggc aagcaaaaag gccattgagg tccagagaat gctgaagttg
    10861 gcaaaggtca ggcagcccag caacgcgcgg gtgcgcagga tcttatcgct gataaacatg
    10921 ctgaaaacgg aaccaagcaa ctgtgggtag ttgaggtggg tttctgattt catttgtggc
    10981 agaccacgcc ataatgccag cgccatcagt gccattaaca ccgaggcaac ccaaaagacg
    11041 gtgcgccagc cgccgaggtt tgccagcaat ccggcaacgg tccgtgcgag taagatcccc
    11101 aatagcaggc cgctcataat ggtgccaacc actttgccgc gtttgtccgg tgaggccagc
    11161 gtcgctgcca gtggaaccag aatttgtgcc acgaccgaga ataaaccggt taatgccgta
    11221 ccgaggatca tcatcgccag tgactgactg ctggcagtga ttaacatacc gcccgctgcc
    11281 agtaaggtca tcgagacaat caggcggcgg cgttcaaaca tatcaccgag gggaacaaga
    11341 aacagcagac ctgcggcata gcccaactgc gcggcggtaa caataaagcc cgccgaactg
    11401 gcggaaaggg aaaagttacg cgcgatggtg tcgagcaatg gctgggcgta atagttactg
    11461 gctaccgcca gaccggtggc gatagacatc agcacgatca gcgccgggct aagctcatga
    11521 ttaggtttag tcattatttt tgttcatcag gtgatgatta agctggaaat caataatagc
    11581 ggatgttgat gaatatggga tgaaagatag attgttgaaa tgtgcggtgt ggtttgtagg
    11641 tatgataaga cgcgccagcg tcgcatcagg cattgtgtac agagcggcgg atgaggcaaa
    11701 aacgccttat ccgcccgaat aaaaattact tctgcgctgc cagcgcttca ttcacccagc
    11761 catcgaactg ctgctggtgg gctttgatcc aaccatcaac gtggccctga atatcgtctt
    11821 ctgaggcttt gccgtcatgc atgatggcgt tctgggcgtt aatatctgcc actggtaact
    11881 gcataatggc aaacagtttc gctgctgccg ggtttttctc ggcccaggct ttgttggcaa
    11941 cgatatgcat ggtactgacc gggaagccgt aattcgcgcc attcggcagt ttggtattgg
    12001 catttttatc gcccggcagt gcggagaacg gcacctgcaa ccagaccaca tctttccccg
    12061 gtttcagttc gttactcacc cagtacggcg tccaggtgta gtaaaacacc ggcttaccct
    12121 ctttgtaacg actgatggtg tcagccatca tcgctgcgta gttcccctga ttatgcgtca
    12181 cggtattggt cagtccatac gcggcaagct ggtggttgat cgcaccttcg cagccccagc
    12241 ctgggttaca gccggttaaa tccgctttac cgtcgccgtt ggtatcgaac agtttggcga
    12301 tcttcggatc tttaagttgc gcgatgttgg taattttata ctggtcggcg gttttcttat
    12361 cgatcaggta accttgtgcc gcgccgttaa caaatacccc ttcacgataa aatttcttat
    12421 cgccaccggc agcttcgtac atgttgtcat gcagtggcgt ccagttcacg gcggtgaagg
    12481 ttgcatcgcc ggaagcaagc gaggtgtagc caacgttgta atccacttcg ctgggtttat
    12541 tgacggtata acctaatttc tccagcgcgc ggctgaccag cagcgtctgg aaggtttctt
    12601 cggtgatggt gctttgaact ggattaactg taatgccttt gcccggcagg tcggcagcaa
    12661 aagtttgtgt agagataagc gtggcaaacg ctgtcgcaaa aagtacgcta tgtcgcattg
    12721 ttattccttt ttttggcagg gtgatatgcc cggtgttacc gggcaagtgc agagttactt
    12781 aatgaatggg cgggtcagca gaccgacagg gccagtggtg taccagcgac ggttgccgcg
    12841 actacgtgag tcgcgcccaa cggcctgcgt caggcgatcg aggataatgg cgaggatcac
    12901 aatcccgacg ccgccaacgg tggcaagccc catatccaga cgaccgatac cgcgaagtac
    12961 catctgaccc agcccgccga cggcaatcat cgaagcgatg accaccatag aaagggccag
    13021 catcagcgtc tggttaacgc ccgccataat ggttggcatc gccagcggta actgaacttt
    13081 gaacagcatc tggctcgggc tggcaccgaa tgagcgcgag gcttcaatca gatccgccgg
    13141 aacctggtta attcccagaa tcgtcagacg gataatcggc ggcagtgcaa agatgatcgt
    13201 caccaccacg cccggcacgt tgccgatacc aaacagcatg acgattggca ccagataaac
    13261 aaacgcgggc gtggtctgca tggcatcaag cagtggacga ataattttcg ccgctcgcgg
    13321 acttctcgcc agccagatcc ccaacggcaa acctatgacg atacagaaca gcagggcggt
    13381 taacaccagc gccagggtaa ccattgcctg tgaccaggca ccgattgcgc cgatggcaat
    13441 cagcgaaacc agcgtcgcca cgcccattcc gaccccggaa atctgccagg cgatgagagc
    13501 gaaaacgata atcgccaccg gcgcgggcat acccagcagc aattgctgga aaccgttgag
    13561 gatgtaatca accggaacgc gcacgccctg aaagacggga cgaaaatggg taacgaccca
    13621 gtcgatccct tcagtgaccc aactgtcgag cgggatcaga gttttatgga acggatcgag
    13681 aatattaaaa tgctcgacgt ttggcgcagg cgtactggtc agccagtcag caccaccgcc
    13741 gtcagtcggt gcagcagccg gtgtacccca ggcgtctgcg gattgtgcag cactgtccgc
    13801 cgctggcgtg gtatcccacg gattattttg atcagccatt atttaccccc tcacgatcta
    13861 aagcgcgcag cagcattcct ttcgaaatga tgccgacata ctgttggtcc tcgtcgacca
    13921 cgggcaccgc acagggtgcc tgtccgacat gagagagcaa ctcgctaaga ggcgtttgtg
    13981 catcgaccgc taacggcgca tcaatcagcg ccgcatcaag accttgctgc tgcgttaacg
    14041 cggctttaag cgaatcgata gacacagcgc cgacaaactt attaccgcgt tcgatgacgt
    14101 agccatattc gcgatcttca tcctgcaata atttcagtgc cgaacgcggg ccgaagccgg
    14161 gggttttacg aattaagccg ttcggtgtac gacgagcaat atctttcgca ctgaacacct
    14221 ggctaatatc gacgccacgg aagaaggtac gaacataatc attcgccgga ttattaagaa
    14281 tttcatccgg tgtaccaacc tgcaccactt caccattttg cataatggca attcggtcac
    14341 caatacgcat agcttcatcg agatcgtggg aaataaagac aatggtgcgc tgatgtttcg
    14401 cctgtaattt taccagctca tcctgcatct cggtgcgaat taatggatcg agtgccgaga
    14461 aggcttcgtc cattaataat atatccggat taatcgctaa cgcgcgggct aatcccacac
    14521 gttgacgcat cccgccagag agttcatccg ggtagctgtg ggcataattt tccagcccga
    14581 cctgacgcag tgcatcaagg gctttttccc ggcgttcttc ggcattaatt ccggccagtt
    14641 ccataccgaa cgcagtattg tccagcacgg tcatatgcgg cattaaggca aaggactgga
    14701 agaccatcgc aatctttttt ctgcgcacct cacggagttc gacgtcggat attttggcaa
    14761 tatccacacc atcaatcagc acttgcccgc gggtgggttc aatcaggcga ttgagaaggc
    14821 gtaccattgt ggatttaccc gaaccggata atcccatgat gacaaatatc tcgccttctt
    14881 caatggccag actggcgtct tttacgccaa gcgatagccc agttttttcc agaatttgtt
    14941 cttttgaaag tccttgttcg atatatttga aagctcgctg tggatgctca ccaaatattt
    15001 tataaagatt tttaatttct aatttaattg ccatgcaata gaaagattcc tgttatttgt
    15061 ttatgtcgat acattaccca atggaaatag tcactttttt ctaccctaac atactgagaa
    15121 tctgaggcaa cccctgatgg caaatgtggc atgaatattg atgtgagcaa atcgccggaa
    15181 tttcccatga tataagggct gagagcaaat cgagaaaagt tgatattttt tgtcaaagat
    15241 agcaaaaaaa cgcctgaata ttgtggtttc aggcgaataa tgcaacagag tatcagtgta
    15301 gatcaccaca aattatttgc gtgatataaa gctatttggc gggaattatt tccctgcagc
    15361 gggtagtgat atttttgaaa ataaccccct cagaaattcc agtcttcatc ttctgtttcg
    15421 accgcttttc ccatcacata tgaagagcct gaaccggaaa agaaatcgtg attttcatcg
    15481 gcattcggcg aaagcgcggc gaggattgcc ggattcactt ccgccatttc tgcgggaaat
    15541 agcggttcgt agcccagatt catcaacgcc ttattggcgt tgtaacagag aaacgctttc
    15601 acatcgtcag cccacggggt ttcggcgtac agctcatcgg tgtattgcaa ctcgttgtca
    15661 tagagttcca gcaacaaatc gaaggcgaaa ctcttcaatt cttcacgtcg tgcctgggag
    15721 atcttttcca ggtttttctg atatttatag ccgatgtagt aaccgtggac tgcttcatcc
    15781 cggataatca gacggatcag gtctgcggta ttggttagct ttccgcggct ggaaaaatac
    15841 atcggcagcc agaaaccgga atagaacaaa aaagattcaa gaaacacact ggcgattttc
    15901 tttttcagcg gatcatcacc gcgataatgt tgctgaataa tctgtgcttt tcgctgcaac
    15961 ggcgcgtttt cttcactcca ggcgtaggcg gcatcgacat ctttggtctg gcacagcgtc
    16021 gagaaaattg aactgtaaga gcgggcatga accgcttcca taaagctgat atttgataat
    16081 accgcctctt catggggggt gagtgcatcg ggcatcagcg agggcgcgcc gataacattt
    16141 tgcagcgtgt cgagcagcgt caggccagta aaaacgcgca ttgttagttg ttgttctacg
    16201 acacttaatg tctgccatgc aggaatatcg ttcgacagtg gcaccttttc tggtagccag
    16261 aaattgctgg tcaggcgatt ccacacctcc agatctttat cgtcagatat cttgttccag
    16321 ttgatggcgc tgatacgtga gagtttcata gatattcctt aaagtgcaca ggagacgcag
    16381 ccttcaattt cagtgccttc cagcgccatc tgacgcaggc gaatgtaata gagcgttttg
    16441 atacccttgc gccaggcgta aatctgcgct ttgttgatat cgcgagtggt ggcggtatcg
    16501 gggaaaaaca gcgtcagcga cagcccctga tcgacatggc gagtcgcttc cgcgtaggtg
    16561 tcgatgatct tttctgcgcc gatttcgtaa gcgtcctgat acagcgccag attttcgtta
    16621 gtcataaacg gggcagggta gtaaacgcgc ccggttttgc cctctttgcg tatctctact
    16681 ttcgccacaa tcggatgaat actcgacgtg gcatggttga tataagagat agaaccggtt
    16741 ggcggcaccg cctggagatt ctggttgtag ataccatagc gcatcacgtc gtcgcgcagc
    16801 tgcgcccaca tctcacgggt aggtaacgta ataccgctac gggcaaacag ttcgccaact
    16861 ttcgccgttt tcggctgcca gttactttgc agatattggc tgaaatattc accactggca
    16921 tagcgtgact gtttgaaccc ggcgaaggtt tcaccgcgtt cgcgcgccag caacatcgag
    16981 gtacgcagtg cgtgccaagt gatggtatag aaatagagat tggtgaaatc gagtgcttcc
    17041 ggcgaaccat aagcgatgcc ttctcgcgcc agatagccgt gtaaattcat ctgccccagt
    17101 ccgatggcgt gcgaggcggc atttccggct tcgatggacg gcacgctgcg gatatgactc
    17161 atatctgaca ccgccgttaa accgcgtacg gcagtctcta ccgtgcgggc aaagtcgggg
    17221 gaatccatgg tgtgggcaat attcaacgac cctagattgc aggaaatatc atggcctgta
    17281 cgggcatagt cgagattctc gtcatattct gaggcgctgt taacctgcaa aatttctgag
    17341 cagagattgc tcatatttat gcgcccggcg atagggttag cacggttaac cgtgtcttca
    17401 tacatgatgt acggatagcc agactcaaac tggatctctg ccagtcgctg gaagaaatca
    17461 cgagcgttga ggtatttttt gcgaatgcgt tcatcggcaa ccagttcgtc atagcgttcg
    17521 ctgatggcga catcggcaaa cggtttgcca taaacccgct caacgtcata gggcgaaaac
    17581 agcgccatct gcgcattctc ttttgccaga tggaaagtga tatccgggat caccacgccc
    17641 agcgacagtg ttttaatgcg gattttttcg tcggcatttt cccgttttgt atcgagaaaa
    17701 cgcagaatat cgggatgatg agcatgtaaa tagactgccc cagccccctg acgcgcgccg
    17761 agttggttgg cataggaaaa tgcgtcttcc agcattttca tcaccggaat cacgccagaa
    17821 gattgatttt caatacgttt aattggcgcg cccgcttccc gcagattcga cagcaaaaat
    17881 gctacgccgc cgccgcgttt cgacagttgt agtgcggaat ttactgcccg gccaatcgac
    17941 tccatattgt cttcaatacg cagcagaaaa caggaaacca gttcgccgcg ctgctgctta
    18001 ccgcagttga ggaatgttgg cgtggctggc tgaaagcgtc ctgacagcat ctcatcggtg
    18061 agctgcgtcg ccagtgtttc atcgccttgt gccagcgtca gcgccaccat cgtgacgcga
    18121 tcggcaaaat cttccagata acgcttaccg tcgaatgtct tcaacgtata gctggtgtaa
    18181 aacttccatg ctcctaggaa tgtctggaaa cgaaaaccgc tggcgtgcgc gtgggcaaac
    18241 agcgtaatga caaaatcgcg agagtagcga ttaaggacac tttcatcata gtaaccttcg
    18301 ttaaccagcc agttcaggcg ctgctgctgg ctactgaagg ccacactgtt cggacgcaca
    18361 tgcgtcgtca taaaggcgtc tactgcctgg cgatctttat cgaactgaat gcgacctgcg
    18421 ctatcgtaaa ggttaagcat cgcattcaga gcgtgataat ccatcgtttc ctgcgtcagg
    18481 cgttctgcgg ttgtcgttgc caaaattcag ttactccttt acgaacgttt tcgatatcgc
    18541 tttgcgtacc catgagttca aagcggtaca gccacggcac gccgcatttc cgggcaatca
    18601 catctccggc gcggccatat gcctcaccaa agttgcgatt acccgaagca ataacgccgc
    18661 gcagcaacgc ccggttatgc tcgtcgttta aaaagcgaat gacctgtcgt ggcaccgcgc
    18721 cagccgtacc gccgccgccg taagagggca cgatcaggat gtaaggctcg tctacctgaa
    18781 tccgttcccg ctcattgagc gggatgcgca ccgcgggcag ccctaaacgt tcgataaaac
    18841 gctgcgtatt ttcggagctg ctggaaaagt agacgagctg gctcatgcac tggccgcgtg
    18901 tggcgctgga tgcagacgat tgatcatgtc cgggcggaaa ccagaccagc taagatcgcc
    18961 tgcaatcaca accggcaact gacgaaagcc ctgagcacgc agcgcttctg ccgcttcagg
    19021 aacgcgatcg acattaatca tttcaaaatc aaagccccgg ttttccatcg cccgtttagt
    19081 ggcgtggcac tgaacgcaat cgttacgagt gtaaatagta atgcgcatga ttcgtatttc
    19141 cgtttaaaat gaagatacgg cgcgatgata cgcgtcgggt tgtctctctg ttgatacaga
    19201 gatactagat gtagttgaaa aaagattcaa ccacacaata tatagcaaat aatggtcagg
    19261 gttaccctgg ggcgagcgtg acggggtagg gggatttttg tgattcacca ggcaaaaaga
    19321 aaccccgaag ccaggcttcg gggtcaaaga cgcgtattta ttatcatttt tgcactacga
    19381 tttgcgcatg cttaacagtg cgccgataaa aatacccacc gctgccgctg tacccacact
    19441 acaccagggt ctttcacgaa caaaagaatc cgcgcagcca acggcatcgc gcgcggcttg
    19501 ctggacgcga gtacgaccat gcattcgcgc cctggtttct ttcagtagag cctgcgcttt
    19561 actacgagcg gcttcggctt cccctttggc gtcgctgccc caggacttca atacagattc
    19621 gagactgtca gctaattgat tgacatcatt ctggatatcc tgcacgccat catcaacgtc
    19681 gttgcggttc ggtcggttaa acatatgatc ccccattaga attcgatgta attttcagtg
    19741 tagaccataa ttttcttaac tgaggttcat cgcggcttta tggacttttc tgaaagcgtt
    19801 gtgaatgcgc aatcaggtaa ggtaggcccg cggttgataa taacgaggat caaatatgta
    19861 tttacgacca gacgaggtgg cgcgcgtact tgaaaaagtc ggttttactg tcgatgtggt
    19921 aacccagaaa gcgtatggtt accgccgtgg ggaaaattat gtctatgtta atcgcgaagc
    19981 gcgaatggga cgtaccgccc tggtgattca tccaacatta aaagaacgta gttcaacact
    20041 ggctgaaccc gcttccgata ttaaaacttg cgatcattac cagcaatttc cgctctattt
    20101 agcgggtgaa cgtcatgagc attacggtat cccgcatggc tttagttcgc gtgttgcgct
    20161 tgaacgttat ttgaatggtt tatttggcga agccagttaa agacgcgact ggcgatgcca
    20221 gtcgcgaaaa gaagggtcag gcttttacct gctggtaacg gctgacttta aacagtcggc
    20281 ggcaataatc gaggaagtag ccataaaccg cccccatcaa catcgaaaca acgatgtttg
    20341 aactgactgc cgccataatc tgatgccagt ctgcgcccac cactaacaag atcgccacat
    20401 acaccggtga ctggaacgtc acataagcca ggatatccgc cagatttttt atccagcctg
    20461 acgggctaac tttgcgtgcc gcgcgcataa acagatcgcg gtacataccg tatggccatg
    20521 caattaagat gttcaccgga atcgctacca atctggaata aaaagactgt tcgaagctca
    20581 ttccggagag gaaaacttca atacacatgt tcacgacaga acagtaaaca accatcgcga
    20641 acgtatctgc aactgcatga cgcaagcgtg actgcggtga gaacatgcta atgctcctta
    20701 ataaaagcgt aatgggatgt taatggagat gaaagcagtg gatctcattg ccatctgcat
    20761 ttttgcatag cgtatatcgc tggctatgac taatcaatta gtgaaaaatt ttcatttaat
    20821 ctgtttttgt agataaaata ttccgtaacg gcgcgttttt tg
//
LOCUS       DACADF010000080        20413 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.081, whole genome shotgun sequence.
ACCESSION   DACADF010000080 DACADF010000000
VERSION     DACADF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.081"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            372..1005
                     /locus_tag="ISB30_002832"
                     /pseudo
     CDS             372..1005
                     /locus_tag="ISB30_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143067.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            complement(1369..1444)
                     /locus_tag="ISB30_002833"
     tRNA            complement(1369..1444)
                     /locus_tag="ISB30_002833"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1409..1411),aa:Thr,seq:cgt)
     gene            complement(1559..2812)
                     /gene="proA"
                     /locus_tag="ISB30_002834"
     CDS             complement(1559..2812)
                     /gene="proA"
                     /locus_tag="ISB30_002834"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP1990446.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADARANGLSEAMLDRLTLTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALNSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADTAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2824..3927)
                     /gene="proB"
                     /locus_tag="ISB30_002835"
     CDS             complement(2824..3927)
                     /gene="proB"
                     /locus_tag="ISB30_002835"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP1990447.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            4216..5271
                     /gene="phoE"
                     /locus_tag="ISB30_002836"
     CDS             4216..5271
                     /gene="phoE"
                     /locus_tag="ISB30_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAP1990448.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(5310..5711)
                     /locus_tag="ISB30_002837"
     CDS             complement(5310..5711)
                     /locus_tag="ISB30_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAP1990449.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(5769..7013)
                     /gene="frsA"
                     /locus_tag="ISB30_002838"
     CDS             complement(5769..7013)
                     /gene="frsA"
                     /locus_tag="ISB30_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAP1990450.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILEVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     ALRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(7105..7563)
                     /gene="gpt"
                     /locus_tag="ISB30_002839"
     CDS             complement(7105..7563)
                     /gene="gpt"
                     /locus_tag="ISB30_002839"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP1990451.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            7824..9281
                     /gene="pepD"
                     /locus_tag="ISB30_002840"
     CDS             7824..9281
                     /gene="pepD"
                     /locus_tag="ISB30_002840"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAP1990452.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFNLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(9337..9951)
                     /locus_tag="ISB30_002841"
     CDS             complement(9337..9951)
                     /locus_tag="ISB30_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HAP1990453.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(9948..11099)
                     /locus_tag="ISB30_002842"
     CDS             complement(9948..11099)
                     /locus_tag="ISB30_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAP1990454.1"
                     /translation="MEWYMGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQ
                     RVVGMPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEK
                     RLSDLDDVAEESWLEENLPSAFAQHPWHNSLGSIGGGNHFAELQQIDQIIDAELFARA
                     GLDAQHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARI
                     NRQLIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGS
                     RGDYSWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICR
                     DKQLIFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(11277..11729)
                     /locus_tag="ISB30_002843"
     CDS             complement(11277..11729)
                     /locus_tag="ISB30_002843"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP1990455.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(11726..12781)
                     /gene="dinB"
                     /locus_tag="ISB30_002844"
     CDS             complement(11726..12781)
                     /gene="dinB"
                     /locus_tag="ISB30_002844"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP1990456.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12852..13775)
                     /gene="lafU"
                     /locus_tag="ISB30_002845"
     CDS             complement(12852..13775)
                     /gene="lafU"
                     /locus_tag="ISB30_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAP1990457.1"
                     /translation="MRKTGNHRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(13778..14641)
                     /gene="motA"
                     /locus_tag="ISB30_002846"
     CDS             complement(13778..14641)
                     /gene="motA"
                     /locus_tag="ISB30_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAP1990458.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(14654..15370)
                     /locus_tag="ISB30_002847"
     CDS             complement(14654..15370)
                     /locus_tag="ISB30_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HAP1990459.1"
                     /translation="MLQDVFESEEFAATLVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWQELAEEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(15390..15857)
                     /locus_tag="ISB30_002848"
     CDS             complement(15390..15857)
                     /locus_tag="ISB30_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HAP1990460.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWSVWHHYAGKGKPTTAAQTES
                     VEALDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMTAPYKDLLVKKVVFQ"
     gene            complement(15865..16926)
                     /locus_tag="ISB30_002849"
     CDS             complement(15865..16926)
                     /locus_tag="ISB30_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HAP1990461.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPVFTLPKMVVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTSLPPQLQELIGQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTPPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLTV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(16923..17234)
                     /locus_tag="ISB30_002850"
     CDS             complement(16923..17234)
                     /locus_tag="ISB30_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HAP1990462.1"
                     /translation="MEKQRRQLFALVEAMNDALDKQRWRRLPALYQQLMRQFHDYAAA
                     ETDAAQLDAVKAQLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(17239..17631)
                     /gene="fliS"
                     /locus_tag="ISB30_002851"
     CDS             complement(17239..17631)
                     /gene="fliS"
                     /locus_tag="ISB30_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAP1990463.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAR
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(17654..18970)
                     /gene="fliD"
                     /locus_tag="ISB30_002852"
     CDS             complement(17654..18970)
                     /gene="fliD"
                     /locus_tag="ISB30_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAP1990464.1"
                     /translation="MINPRTIAQEIAYADVATQATNLQEKQSELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ASGPAITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSSSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDAMKSDPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(19380..20294)
                     /gene="lafA"
                     /locus_tag="ISB30_002853"
     CDS             complement(19380..20294)
                     /gene="lafA"
                     /locus_tag="ISB30_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HAP1990465.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
BASE COUNT         4717 a         5562 c         5101 g         5033 t
ORIGIN      
        1 aacctgacca atacgcatat cactaattgt gaaatcatat gccccactcc tgatgttgtg
       61 gcgttattcg agaataaaat gatgccaatt tataataaac tggcatcaaa cactcaagag
      121 aatagtcatt taatccaact ccgcgactgg ctcctccctc tacttatgaa cggacaggtc
      181 acggtcaaat aatccccggc gtggttttgc gaggcgcatt ccagatccgc attgcgccgc
      241 actcaccgca gcaaaaccgc ttgccgttca taaccgatcg cagtatcttt ctcccgcgcc
      301 tgcaaaatca ggcgtcggga ttagcctcct gaaaaccgtt acaggcgaca cttgacgcac
      361 cagcgtcttt tttgtgtcgt gcgttcggct acacctcaat ggcgggccgg gcgggggcgt
      421 cgcaagacgc gccggtttcc tgtaacgccg gtaaggctaa ccccgtccgg ttccgccccc
      481 atgagattag cctctctagt ggcggacata ataaaccgtt acaggagacg ccctatggct
      541 acaatcctca tctctgactg cgatttactc gatctattgt ccgctccctt caatgccacc
      601 acggatttca ccgtcctggc cgactactgc gcccgcttcg ccgaaaccct gatagaaacc
      661 gacgatccgg cgctgcggct ggcgctctgc ggacggcttc acgcggctct tgcgctattg
      721 caacccacgc tgcacgaccc catcccgccg catctgattg aaagcctgac cgttgatacc
      781 cttcccgcca caccagagca ctttatttcc gaaccagatt ccttgtgcga atactgcctc
      841 gcgctgctcc agctactgct ggaacgtcca gcagagccaa aaacggaaca aaccctgacc
      901 gggcttctgt gtgagctggt ctggaatttc acggaaggac tcaaatcccc gcgctggctg
      961 cgtaccgccg acggcgtaaa atttatcagt gaggtggccg catgatgcaa caggactgac
     1021 attccgccga cattattgcg gtgctgaaaa agcggggaac gcctctggcg gcacagagaa
     1081 aaactcattg cggaagcgct gtaaatatcg ccagaaatca tctggtcctc aaggtacttt
     1141 gatagcaaag gcaagctggc tggttcagtc cggagtctct cttcttgcac ttaaagagat
     1201 gggaggatgg gaaactcttg aaatggttcg tcgttaggct cacctttcag ccaggcattt
     1261 caccgggcac gcgagcaaaa tcgatgcgat tataagtcgc aatggcacaa atacggcaca
     1321 agaggagaac gtggtgtatt taaatatggg gtaactactt gattttaatg gtgccgataa
     1381 taggagtcga acctacgacc ttcgcattac gaatgcgctg ctctaccaac tgagctatat
     1441 cggccctgaa aggacatgtt cacgaacgtg aatcacggtg gacaaggtta aaactaaccg
     1501 ggcgatgcgt caatggcctt gtgaatcaaa tggctacttt tgcatcaccc ggttttattt
     1561 acgcacgaat ggtgtaatca ccaatgccga tccacttgta agtggtcagt gcttccagcc
     1621 ccattgggcc gcgcgcgtgg agtttttgtg tgcttaccgc cacttccgcc cccagtccaa
     1681 actggccgcc gtcggtaaaa cgcgtagagg cgttaacgta aacagcggag gaatccactt
     1741 cgttaacaaa acgctgggcg ttgcgcatat cgcgggtcag gatcgcatcg gagtgttgcg
     1801 tgccgtgttc acgaatatgg gcaatggcgt catccagatc gctaacgatt ttgacgttca
     1861 aatctaatga cagaaactca tcgtcatact cttcggcttt aacagccacc accttcgcgg
     1921 ggcctgcctg caactgcgcc agcgcggctg tatcagcgtg taacgtcacg ccgctttccg
     1981 ccatttgttt gcttaatgcg ggcaggaagc tatcagcgat gtttttattc accagcaacg
     2041 tttctaccgt attacacgtg ctcggacgct gagttttcgc gttgacaatt actttcaggg
     2101 cttcagcgat ctctgcgctt tcatcaacgt aaatatggca tacgcctata ccgcctgtga
     2161 tcaccgggat tgtcgactgc tcgcggcaca gtttatgcaa accagccccg ccgcgcggga
     2221 tcagcatgtc gatgtattta tccatacgca gcatttcact gaccagcgca cggtcaggat
     2281 tatcaatcgc ctgcacggca cccgccggta agccacaaga gttcagggcg tcctgaatca
     2341 ccgccaccgt tgcagcgtta gtgcgacacg tttctttgcc gccgcgcagg atcactgcgt
     2401 tacctgtttt caggcacagc gaagcgacat caaccgtcac gttcgggcgc gcttcataaa
     2461 tcacgccaat aacccccagc ggtacgcgac gacgctcaag acgcaggccg ctgtccagta
     2521 cgccgccatc gattacctgc cccaccggat cggcgaggtt gcacacctgg cgcacatcat
     2581 cggcaatgcc tttcagccgt gcgggcgtca gtgtcagacg gtcaagcatc gcttcgctaa
     2641 ggccattggc acgcgcgtct gcaacatcct gcgcgttagc gttgaggata ctttcgcttt
     2701 gtgcttccag ttcatcggcg attttttcca gcacacgatt tttttcgcgg ctggagagtt
     2761 gcgctaattt atacgaggct tgcttcgcgg caatgcccat ttgttccagc atcggccagc
     2821 tccttaacgg gtaatcatgt catcacggtg aacggcaacc gggccgtatt catatcccag
     2881 tattgcatca atttcttgcg agtggtgtcc ggcaatacgg cgtaatgcat cgctgttgta
     2941 acgactgacg ccgtgggcga tatcgcgacc ttcgaggttg caaatacgga tgacttcacc
     3001 acgcgagaaa ttgccagtca cgcttttaat gcctttcggc aacagggagc tgccgcgttc
     3061 cagaatggcg gcagttgccc cttcatctac cgtgatttca cccgcaggcg gcgcaccgaa
     3121 aatccagcgt ttacggtttt caagcggagt cgcctgggca tggaacagcg taccgacgga
     3181 aatgccttcc atcacatcac caataacgcc cggcttgctg cccgcggcaa taatggtgtc
     3241 gatacccgca cggcaagcca cgtcagcggc ctgcaatttg gtgctcatac cgccagttcc
     3301 gaggcctgaa acgctatcac cggcaatcgc gcgcagtgcg tcatcaatgc cgtacacatc
     3361 tttaatcagt tctgcctgcg gattgctgcg cgggtcagcg gtatacaaac ctttttgatc
     3421 ggtcagcagc aacagtttat cggcacccgc cagaatcgcc gccagcgcag aaaggttatc
     3481 gttatcaccg accttaatct ctgccgtagc gacagcatcg ttctcattaa ttaccggaac
     3541 gatattgtta tcgagcaacg cacgcagggt atcgcgggcg ttcaggaagc gttcacgatc
     3601 ttccatatca gcacgggtca gcaacatctg cccgacatga atgccgtaaa ttgaaaacag
     3661 ctgttcccac agttgaatca gtcgactctg ccctaccgcc gccagcagct gttttgaagc
     3721 gatagtcgct ggcaattccg ggtaccccag gtgctcacgt ccggcggcaa tcgcacccga
     3781 cgtcacaata acaatccgat gcccggcggc atgtaactgc gcgcactggc gaacaagttc
     3841 aacgatatgg gcacggttca ggcggcgcga tccgcctgtt agcacactgg tgccgagttt
     3901 taccaccagc gtctggctgt cactcatgat tctctgccat tcaattttag gaaaaaatga
     3961 tatcaaacga acgttttagc aggactgtcg tcggttgcca accatctgcg agcaaagcat
     4021 ggcgttttgt tgcgcgggat cagcaagcgt agcggcagtt gtttacgctt ttattacaga
     4081 tttaataaat taccacattt taagaatatt attaatctgt aatatatctt taacaatctc
     4141 aggttaaaaa ctttcctgtt ttcaacggga ctctcccgct gaatattcgc gcgttaatta
     4201 aaatcaggaa tgaaaatgaa aaagagcact ctggcattag tggtgatggg cattgtggca
     4261 tctgtatccg tacaggccgc agaaatatat aacaaagacg gtaataaact ggatgtctat
     4321 ggcaaagtta aagccatgca ttatatgagt gataatgaca gtaaagatgg cgaccagagt
     4381 tatatccgtt ttggttttaa aggcgaaaca caaattaacg atcaactgac tggttatggt
     4441 cgttgggaag ctgagtttgc cggtaataaa gcggagagtg atactgcaca gcaaaaaacg
     4501 cgtctcgctt ttgccgggtt gaaatataaa gatttgggtt ctttcgatta tggtcgtaac
     4561 ctgggcgcgt tgtatgacgt ggaagcctgg accgatatgt tcccggaatt tggtggcgac
     4621 tcctcggcgc agaccgacaa ctttatgacc aaacgcgcca gcggtctggc gacgtatcgg
     4681 aacaccgact tcttcggcgt tatcgatggc ctgaacttaa ccctgcaata tcaagggaaa
     4741 aacgaaaacc gcgacgttaa aaagcaaaac ggcgatggct tcggcacgtc attgacatat
     4801 gactttggtg gcagcgattt cgccatcagt ggagcctata ccaactctga tcgcaccaac
     4861 gagcagaacc tgcaaagccg tggcacaggc aagcgtgcag aagcatgggc aacaggtctg
     4921 aaatacgatg ccaataatat ttatctggca actttctatt ctgaaacacg taaaatgacg
     4981 ccaatctctg gcggctttgc caataagacg cagaactttg aagcggtcgc tcaataccag
     5041 tttgactttg gtctgcgtcc atcgctgggt tatgtcttat cgaaagggaa agatattgag
     5101 ggcatcggtg atgaagatct ggtcaattat gtcgatgttg gtgctacgta ttatttcaac
     5161 aaaaatatgt cagcgtttgt tgattataaa atcaaccaac tggatagcga taacaaattg
     5221 aatattaata atgatgatat tgtcgcggtt ggcatgacct atcagtttta atggatattg
     5281 ccggatgtga tgcatccggc acatttcatt cacgccgtta acttcaccgg ttcatcacga
     5341 aaatcatctg ccggttccag cttcagattc agcgtcgcca gcagctcacg cagcttctcg
     5401 tgaaattcac gcagggtgtg ctccagtcgt tcaaccactt cggcatcttt tatctgaaca
     5461 ctcgtccagt cgcctgcttt atcgaacaga ccaaactggt aactgtaggt aaaacgggat
     5521 tcctgcgctt ccagttccat ccaccagccc cagaactcac gcacttccgg tgccggtttc
     5581 acgttgacgc atacagccag acaatcgaaa aagaatcgat tatcttcgca cttaccttca
     5641 cgaatatacg ggcctagtgc ggtaaatttt ttgactaatc tgctcttcgg gtgtccactc
     5701 ggtaacgtca ttgcaatctc ctgttgtgat gcaactgttt taccaaattg gtaaaattta
     5761 gcaaattttt aacacaaacg tttttcgatc cagtctgtga tttcctgaag gcctttgtca
     5821 aaattccgat acaccgggtt aaatgggatc tctaataatt taccgtcagc agatgacgac
     5881 gtgattaagc gtgagtcctc ttccgggctg aacggatcgt tcttccagta gcctgataac
     5941 attggcgttg ggcagcgacg tcccagcaac ccttgcactt ttaatgaata gcgattcagc
     6001 tccacgcgca acgcttcgtc agaagcatca tgcatcccca aacgactcgc cagaacgtca
     6061 agatacattt ccggcacctg ttgctggcac ttaaaatcac tcaacagggt atgaactacc
     6121 ggaccaagac aggcaaccgc tttcagacgc ggcgattcaa ggtatgccag acgtacggca
     6181 acgttagcgc cgaaacggaa accaaaggcc gcgacgcgag tgtgatccac ccacggtacg
     6241 ttaggcaacg cctttaaaac gtgctgatgc aacaggctgg agtcctgggt gagcttccat
     6301 tttgaagaaa agcccaccga cggcatatca atagtcagca tcgcaatgcc gcgcagcgca
     6361 aaataacgtt catacaggct gtaatagtct gtctgcatcg catccagacc accacacatt
     6421 aatactgtcg ggaacgggcc atcgcctttc ggcatatgca aaaagccggt gatgggcgca
     6481 ccgccgggta cggtaaactc catctgccgc atcgtgcccg gcagacgctg agcggcctct
     6541 tcataggcgc ggtttgacaa agcctgcgct tgctcggcca ggtcatctcc tttcagatga
     6601 ggataggcag caatgttgta caacgtagcc gcatgcagcc agtgtcgacc gctgagttgc
     6661 ggatcttctt ccgcacaggc tttttgttgc cacaccatcg cctgggtagc ccactcataa
     6721 atccagttgc cgccgcggta gccaatcacc gtatcgtata aatcatcgtc ggtacgttcg
     6781 gcatcgctca tcacaatacg cgcctggact tcgaggattt cacgggggtc aatgccgcgc
     6841 cagatccaca tcagacggtt aatcatgcga taccagtgag ggatggtttt accatcaagg
     6901 gccgactgca caggcagttg tgcgccgtga ctaaagcggc ggactagcgt cgaggtttca
     6961 ggatgtttaa agcggggttt gaacagggtt tcgctcaggt ttgcctgtgt catagatgca
     7021 gcctccagaa tacttactgg aaactattgt aacccgcctg aagttaaaaa gaacaacgcc
     7081 cggcagtgcc aggcgttgaa aagattagcg accggagatt ggcgggacga atacgacgcc
     7141 catatcccac ggctgttcga tccaggtatt ttgcgggata tcaacaacat aatcatcaac
     7201 cagcggacga ccagccggtt ttgcgaagat ggtgacaaag tgcgcttttg gatacatttc
     7261 acgaatcgca accgcagtac caccggtatc taccaggtca tcaataacga tgaagccttc
     7321 gccatcgcct tctgcgcgtt tcagcacttt aagctcgcgc tggttgtcgt gatcgtagct
     7381 ggaaatacaa acggtatcga catgacgaat acccagttca cgcgccagta acgcacccgg
     7441 taccagaccg ccacggctta cggcaataat gcctttccat tgttcagaag gcatcagtcg
     7501 gcttgcgagt ttacgtgcat ggatctgcaa catgtcccag gtgacgatgt atttttcgct
     7561 catgtgaagt gtcccagcct gtttatctac ggcttaaaaa gtgttcgagg ggaaaatagg
     7621 ttgcgcgaga ttatagagat ctggcgcact aaaaaccagt atttcacatg agtccgcgtc
     7681 tttttacgca ctgcctctcc ctgacgcggg ataaagtggt attctcaaac atatctcgca
     7741 agcctgtctt gtgttgacaa cattttctgc taaccctgtg acctgcaata ctgttttgcg
     7801 ggtgatcgac aaggagactt aacgtgtctg aactgtctca attatctcca cagccgctgt
     7861 gggatatttt tgccaaaatc tgttctattc ctcacccgtc ctatcatgaa gagcaactcg
     7921 ctgaatacat tgttggttgg gcaaaagaga aaggtttcca tgtcgaacgc gatcaggtag
     7981 gtaatatcct gattcgtaaa cctgctaccg caggtatgga aaatcgtaaa ccggtcgtct
     8041 tgcaggccca cctcgatatg gtgccgcaaa aaaataacga caccgtgcat gacttcacta
     8101 aagatcctat ccagccttat attgatggcg aatgggttaa agcgcgcggc accacgctgg
     8161 gtgcggataa cggcattggt atggcctccg cactggcggt tctggctgac gaaaacgtgg
     8221 ttcacggccc gctggaagtg ctgctgacca tgactgaaga agccggtatg gacggtgcgt
     8281 tcggcttaca gagcaactgg ttgcaggctg atattctgat taacaccgac tccgaagaag
     8341 aaggtgaaat ctacatggga tgtgcggggg gtatcgactt cacctccaac ctgcatttag
     8401 atcgtgaagc ggttccagct ggttttgaaa ccttcaattt aaccttaaaa ggtctgaaag
     8461 gcggtcactc cggcggtgaa atccacgttg gcctgggtaa tgccaacaaa ctgctggtgc
     8521 gcttcctggc gggtcatgca gaagaactgg acctgcgtct tattgatttc aacggcggca
     8581 cactgcgtaa cgccatcccg cgtgaagcct ttgcgaccat tgctgtcgca gctgataaag
     8641 tcgacgccct gaaatctctg gtggatacct atcaggagat cctgaaaaac gaactggcag
     8701 aaaaagagaa gaatctggca ttgctgctgg actccgtagc gaacgacaaa gctgcattaa
     8761 cggcgacatc tcgcgatacc tttattcgtc tgctgaacgc caccccgaac ggagttatcc
     8821 gtaattccga tgtagccaaa ggcgtggttg aaacctccct gaacgtcggt gtggtgacca
     8881 tgaccgacaa taacgtagaa attcactgcc tgatccgttc tctgatcgac agcggtaaag
     8941 actacgtagt gagcatgctg gattcgctgg gtaaactggc aggtgcgaaa accgaagcaa
     9001 aaggcgcata tcctggctgg cagccggacg ctaactctcc ggtaatgcat ctggtacgtg
     9061 aaacctatca gcgtctgttc aacaagacgc cgaacatcca gattatccac gcgggcctgg
     9121 aatgtggtct gttcaaaaaa ccgtatccgg aaatggacat ggtgtctatc gggccaacca
     9181 tcaccggtcc acactccccg gatgagcaag ttcatatcga aagcgttggc cattactgga
     9241 cactgctgac tgaactgctg aaagaaattc cggcgaagta attatttgat ttgctgccgg
     9301 atggcgttta acgccttccg gcagtttcat ccttcattat ccttcgataa aagccatccc
     9361 tgtgaacgtt cgtcgcgggt tgccacgttc aatctggtga tggaacattc gccgctgcga
     9421 tttcagcgcc gcgctatttt cctgttgctg ttgttccagc ttccaggcaa tcagtaatcg
     9481 cgccagccgt ttgttagcat gctgactgcg ctctgactga accttcacgc taataccgga
     9541 tgccagatgc gtggcgcgta ccgccgagtc ggttttattg acatgttgac cgcccggccc
     9601 cgacgaacgc agcgtctcat aacggattgc atccgattgt tcctgctcat cagcggtaaa
     9661 acgcccaatg cccagaaacc agtttttgcg cccatgatga ggccgatacg gactcggaca
     9721 aatccactga atagtgccgc accagctttc gcttagagcc catgcgttat cgccatccag
     9781 agaaatcagc gccgaacgca gtgtgtcaga gtagcgaccc gtttctgttt ccagcaccgt
     9841 taccgcgacg tcctgtcggg tagcttcttt aatcagcctg tccagtgctt ttttcactgc
     9901 gagacaacat tcttccggcc cctgagcaga ggagagttgt agcaagatca tccttttttc
     9961 cctccactgt ttttgagcgt cagcaccggg cgcagtcgcg cgacaggaat aattaaccca
    10021 gccagcacca gacattgcac cacgctttca gccgatttat aggcttgtgg cgcttcttca
    10081 aagatgagtt gtttatcgcg acaaattacc cgactgccaa gttcagtccg cgagagttgc
    10141 gtcgccgtgt atttcgctgc cagacgccct ttacactcgg tgcgccccca tttacgcccc
    10201 gccccatgcg ccagcgagtg gagcgttttt tcgttcgcaa caggtttaac cagccaggag
    10261 taatcgccgc gtgaaccggg aataatcacc agaccgttgt catccggcgt ggcgccttta
    10321 cgatgcaacc agccctgttg atcgccgatt tggcacgcgc taacaaagtt atgcgccaca
    10381 tccagaaccg gacttccggt ggccttaact tgttgcatta tgcgcaaagc gatcagctgg
    10441 cgattaatac gcgcaaacgc cagcgcatcg tcatgttccg caatataccg tagcgcgtcg
    10501 tcactgcctt caggcaaacc atgatgcgaa aacgaggcaa tatgccgctg taaaatagac
    10561 tgccccaatc ccctcgagcc gctatgaacc agtagttgca gatgctgcgc atccagacca
    10621 gcccgtgcaa acagttccgc gtcgataatt tgatcaatct gttgcagttc tgcgaagtgg
    10681 ttaccgccac cgatggagcc aagcgagttg tgccacggat gctgtgcaaa tgctgacggc
    10741 aggttttcct ccagccagct ttcttcagca acgtcatcca gatctgataa tcgcttttca
    10801 aacttatcgg cgttgtattt gcgagcgaga atatctgttt gccatagcgc catgccgcag
    10861 ccgatatcat tgccgaccag tgccgggtaa aaacgaccaa cagagaagaa cgctgcgcca
    10921 atcgggtagc cgcgtccggg atgtaaatct ggcatcccca caacgcgctg catgttgggt
    10981 aaatttgccg tggtgtgtaa ttgttggatc gctaaactct cgatccacag gtcatcagat
    11041 gcgatggtaa ataccgcatc ggataaggga cgaatatatt tgcccatgta ccattccatt
    11101 tatgaataaa aatcaggaaa tggcagacga atccagggca agaaaaaaga attaatcctg
    11161 gaaaggtctg caaagagtca ttgataaagg atttgtcatg atttacctcc ttgcagtttg
    11221 tggaataaga aaacgcgcac atactagacg ggcgttattt tcattgcaag ctggatttaa
    11281 tgttgcggtt tatatcgcat ataaaaatta gtaaaccacg ttccccggca ttcaacgcgc
    11341 tgctgcttaa ctgtctgaaa accataacgt tcaaaaaagg gttttgcggt tatgctggcg
    11401 tccaccgtaa gttcggattc agacttaatc aatggtttta acaaagcgct ggcaactccg
    11461 cgtcgggtgt attccggttc aacaaataac atatcgatat aatgtccaat gcgggtaata
    11521 aaaccaaccg gttgtgcatt aataactgca acccgcactt gtgatttcgc gagtttctcc
    11581 ttccagcgag attcgtcaat ctgcgcccag gcggcaattt gctgtggaga atagtgctga
    11641 ctcgccgtca tcgtaacagc tcgaaggaaa atagcgcata gttgctgaaa atcaccaggc
    11701 tgatagtttc ttatttgtat gttattcata atcccagcac cagttgtctt tccatttgcg
    11761 ggtcaagcaa cgtcacatgc agccccacca gtcgcacacc gcgcccgccg cggcgttcat
    11821 cccaggtttt acgcgcggtg gcgattagat cagatttatt cagccgcggc cagacgtgtt
    11881 cctgggtggt ttgctgaaaa tcgtcgaact ttaatttcac cccctggcgg gcaatcagca
    11941 aatcaggttt tacctttgcc aaacgacgct ccagttcagg gtaaagccgc tcgatgatcg
    12001 cttcacattc agaccagtga tgaatatctt ccgccatcgt acgttctaca ccgacggatt
    12061 ttcgcaacct ttcgctgtta acgtcgcgtt cgtcaatgcc ctgactacgc tcccacaaaa
    12121 tgcggccaaa tttgccaaag cgtttaagca gcatcaccag atcacacttt tgcacatcac
    12181 cgcaggtccg cagccccatc gcttccagtt ttgccgccga gactttgccg acgccgggaa
    12241 tttttgccag cggtaaggtt tgtaaaaatg tcggaacttc tgccggcgta atcacaaact
    12301 ggccgttggg tttattcatg tcggaggcga ttttggcgag aaactttact ggtgccacgc
    12361 ccgcagacgc cgtcagttgc agctcgttga agatcgtctg gcggatttcc tgggcgatga
    12421 gggtcgcaga accgtggcaa tggacgctat cggtgacatc gagataggct tcatccagtg
    12481 ataacggttc aatgcgcgag gtatagcgcg agaaaatttc gcggatatga tttgaggctt
    12541 ctttgtaggc gtcaaagcgc cctggaagca aggtgagatg cgggcataat ttgagcgcca
    12601 tccctgtcgg catagcgcta cgtacgccaa atttacgcgc gggataattg gcggtgctga
    12661 tcaccccccg tcgttcgcgg ctgccgccaa tagcaatagg gatatcgcgc agggcgggat
    12721 tgtcgcgcat ctccaccgct gcgaaaaagc agtccatatc cacatgaatg attttacgca
    12781 ttgctcacct ctcaatactg gtaaagtata cagtgatttc agggtttgag aaatgcgtaa
    12841 agattcagca tttaacgcgt tcgctgcgaa agcacctgct gcttatccag cttttgcacc
    12901 agcggctgca ccactttatc cccatgctga ccaaagtatt gatacagcgt atcggacgca
    12961 cttttggtca gcaccataat ctcaatgcgc cggttgcccg cgctttgcgg atttttggca
    13021 tccagcagca tctggtccgc cattgcactt acctgcatca ctttatcttc cggcattccg
    13081 gcctcttcca gcacccgacg agctgaaagc gcgcgatcgc ccgaaaggtt ccagttgttg
    13141 tagatattgt ttttgtaggc catcgcatcg gtatgcccgg taataataat tttgttatcg
    13201 agcgagtcga acactggcgc aagctccacc agcaaagttt taaagaacgg cataatcttc
    13261 gcgctgccgc gttcgaacat attgcggttc tggtcgtctt taatcagcac gcgtaatccc
    13321 tggggaacaa tctccatctc cagattcgct tccatatgcg catcacgggc gatggtattg
    13381 atactggttg ccagctcgcc aagctcggtg gcggatttct tcttcagcag cgccgttttc
    13441 tcgttgacgt cgcgcgcttt tttctccgtc tcttccggca ccgccacggt ggcaggtttt
    13501 ggcggatgag acgggctgat tttatttaac ggcgacaacc caccaccgtt aaaaatcgac
    13561 tgcccgtgca gcgcggcgat aatgctttca cgctcggatt tactgacgct gttgacaatc
    13621 cacagcgtca taaatagcgc catcattgcc aaagtaaagt cggcgaacgc caccttccac
    13681 gccccgccgt gatgcccggc atgattcttt ttgatctgcc gccggacgat ggtggttttt
    13741 gccccacgtt cgcggtggtt gcccgtcttt ctcatgctta ttcttgctcc agcatcgcat
    13801 tcacccacgc gtcgaggttg gcgaaggtcg gtttggacgc cagatgcagc agcttacgcc
    13861 cggcgtccac cgccagcagc gtcggtttgc cgcctgcctg cgccaccagc acagtgcgca
    13921 cgcattccag caaagaatgt tccgcccgcg cctgctgttc catggcgttg gctagcgggt
    13981 ccatcaggca gtagcagata aacacgccta aaaacgtccc caccagagcc gccgccactt
    14041 tcaggccaat cagagcgata gatccatcaa tcgactgcat ggtgatgatg atgcccagca
    14101 ccgccgcgca aataccaaat cccggcatcg cttcggcggt acgttgcagc gagcgcgacg
    14161 gcgttaacag ggactcttcc gcggtgtcca gttcctgatc gagaatgcct tccagctcat
    14221 gggcgtcgat tttacccatc gccatcaggc gaaagttatc agcaataaag gtcaccagcc
    14281 gcttctgggt taacaccagc gggtatttct ggaaaatggt gctctcttcc ggctgctcaa
    14341 tatgctgatc caacatccgc agaccgccgt tttgcaccat ttcgagcaac tcatacaggc
    14401 acattaatag ctgacgctga aactccggcc cgagctgttt tttactgatc acgcctttaa
    14461 tttgatgggc aatctctttc agcacatgtt tcgggttacc aataatcatc gccccgaaac
    14521 cggcacccaa aataatgatc atttccgccg gttgccagat agccactaat tgcccgcccg
    14581 cttcaaaata accgccaaat acgcagccga gcaccaccag taaacctaat attttttgca
    14641 tgatttcttc actttaattc ggatagacaa aatcacgaat tttctgcgcg atctgtttat
    14701 tcagttgaca aattcgggcc tcggttagcc ctaataccag cgcaatctct ttgagattca
    14761 tctcatgctg gtaatacatg gagaggatca gttgctcttt ttcgcttaat tgtgaaagcg
    14821 cgtgttgcaa catttgctga gtgacaaact gatcttccag ttcccggcca gcaagcggaa
    14881 tgcctggccc ttcgccgttc agcagttcgt ccaggctggc gagcgtttcc gccccttcca
    14941 gttgctgata ttcgagataa tcttcatgcg agatcccttc ctcagccagc tcctgccact
    15001 ccggttcgtg ccccagtttt ttacgcgttt cgcgaatcag gtctttcatc tgatgatatt
    15061 tctgtcgcag ctggcgcggt cgccagtcga gagcgcgcag ctcatccagg atcgcaccgc
    15121 gaatacggtg cacggcatag cccgcaaaac cttcgtccgg cagcccatag cgacgaatgg
    15181 cgttcaacag ccccattaac gccgtttgtt ccatatcctg acggtcaata atgcaattac
    15241 actgcggggc aagttgccgc accactttac gcaccaacgg taaataggcc tgcaagtagt
    15301 ggctctcctg ctgcggagtt aataccagcg tggctgcaaa ttcttctgac tcgaatacat
    15361 cctgtagcat gtttatatcc gcctggtgat tactggaaga ccactttttt caccagcaaa
    15421 tccttatacg gtgcggtcat tttgcgacgc tgaagatctt tctttaacgc gtcagaaatc
    15481 agtttgcgga tggcagatat tttcatgccg cgcaaatctt caaatttcat ttccgtcagg
    15541 cattcaaccg caatactttg atacagcggt tcctgatgct ttattttttc cgcctctttg
    15601 tcactggcga ccaccatcac taattccgcc gacaaataat gatccgcgcc gttgttgtca
    15661 tgcaaagtga caatggtttc cggcagcgtc acaaatacgc tcttgctctc atccagagct
    15721 tccaccgatt ccgtttgcgc ggcggtagtc ggttttcctt ttccggcata atgatgccag
    15781 acactccatc ctgctccagc gccgaccacc aacgccagaa cggcgctgac cacgcctgcc
    15841 accacgattt tcttcatatt gcctttacac tttgatcaaa acggattcgt tctgttgaaa
    15901 ttgaggcgca tcatcaagct gtaaggcggc actcacctct tcctgctgat ggcccgactg
    15961 ttgttgctgc tgcgactgcc cttcggagga aacctgtacg ttcacctcca taaaattttg
    16021 cgccgtcaga tgctggcgga gatcgtcgct aaactgctgc aatgcgcggc agacttcgct
    16081 ttgattcgca ccgatatgga ccgtcagttt gccgttatcc aactgtacgg cgatctcaag
    16141 cttgccaagc gacggtggat cgagacgaat ggtggagatc tgctgttgtt tgttgagctg
    16201 aaagtggatc tgatctttca acagcgtcgt cagtttttcg cccagttcgt ccatcgaaag
    16261 cgtggctggt ttggcgacaa tctcctgatg atcgctgacc aactctggcg tcttaacctg
    16321 caacaccgca cgcgcaataa ccggttcgct gtccggcact ttttccgttt tacgctcgac
    16381 ctgcgggcgc gcggtcacac gggttagttc aggtttcggg cgagctggcg gtgttgagac
    16441 gcgcggctgc gtcggcgcaa tggcatgtaa atcttcactg gcgtaagtcg ccagtctggc
    16501 ctgctgttcg ggtttcgcct gcggcaactg accgatcagt tcctgcaact gcggcggcaa
    16561 cgacgtgagt tcctgctgct gcgtcgggcg ttgcggcgcg ttttgcacca ccgctttcgt
    16621 caattgctgg ataaccggcg atgttgcgac gtttcgcggc tgcggcgtgt cctgatgcgg
    16681 cgcggcaggt tgcggaagta acagcgccat tagcgcctgc atcgccaccg gatcgttctc
    16741 ctgcgggcgc gtcgcctgtt gctgactggt tttagcgcta tgaacgcgct ccgccaccgc
    16801 cgcgaccacc attttcggca gggtaaacac gggcgcgttt tcaccgctca ccggcggtaa
    16861 aatatccgcc ttcaacgaag cggtttccgc aagcgttccg agcgtggcaa gcaatgccgg
    16921 attcatgact tctccgaaag taaattaaca attgagtagg ccagtacgcc ttcctgatgc
    16981 cgctgatgct gttccatacg cgctttcaac acttctgcgc gctgctcgcg ccgtttaatc
    17041 agggcattaa acgcgccgta cagttgtgcc ttcaccgcat ccaactgcgc cgcgtcggtt
    17101 tccgccgccg cgtaatcgtg aaactggcgc atcaactgct gatacagtgc cggaaggcgt
    17161 cgccagcgtt gcttgtcgag ggcgtcattc atcgcttcga ccagtgcaaa aagttgcctg
    17221 cgttgttttt ccatcgtctt aacccagctt gcccgaaagc ccttcccagc cttcgcgcag
    17281 gttgcttaag atcagcatca cttcatcgat tttctctgcc gacagttcgc cgctggcttc
    17341 atacaagcga tacacacagt ggtcatagag gcgcgaaagg ttgaccacca gctcgccgcc
    17401 ggtttcaaat tccagcgaac tggtgagcgc attgagaatg tcgatgcatt tattgatgct
    17461 ctgggctttc ttctcgaaac gccgcccttc aatgtggctt ctggcgcgtt ccagctcgtc
    17521 catcaggcca gaaaacagca ccagcaccag ttcaagaggc gatgccgccg cggtacgcgc
    17581 cgcgagatcg atctctttgt attgcgaata accgtcctgt acttggtaca tctttaagga
    17641 tattccttct gaattacgca aatgccgcca tactggcttt catggtgtac acctcaacca
    17701 acgtattggt gtactcctcg agataacggt cgtagttagc gttataggta tcggtaagct
    17761 gatcgccttc gtcctgaatt ttgctcatct gatcatcaat gttctgctgg cgcagggtga
    17821 tgatgccgtt gctggagtcc gtgtaggtgt taatcaggtc gtccatctgc gccaccatgc
    17881 tgttgtcgcc aacgaaaata gaggtcaggc cgtcgggatc gctcttcatc gcgtcgttga
    17941 actggtcgga gtcaatctgt aagtggccgt gagaatcgag cgtgatgcca tagtcgacaa
    18001 tcgacacgcc gttgtagctg gcatgggcga tgtcatcgag ctggtttgcc agtgagctga
    18061 gtccagcatc ccccgcaaag accccggcgc tactactgtc atcaccgtgg gtggttagcg
    18121 aatcaaccgt atcaatcaag gtgttatagg catcgacaaa ggtctgtact ttctcctggc
    18181 tggcgctgga atcttcactg atgttaaagg tggtgagatc gctgtcagaa tcgctgactt
    18241 cggtaaaagt catggtgaca ccggggatca catcatcaaa cgtgttgctg ctgttggtga
    18301 tagccggtcc gctggcgcta ccgagatgaa taatcgcgtc ctgagcggag gaaacatccg
    18361 tggcaacggg cgcactggtg ctatcgttgc tggcatcatg ccccgttacg ctcaccgaaa
    18421 acgcgctctg cgccccggtg ctatcggagg tgagcataat cgtggtggtg ccgtcggttt
    18481 ttaccagtgc cgccgacacg cccggattgt catcggagtc gttaatcgca ttcaccagct
    18541 cgctggcgtc gataaaaccg tcaccgtcac cgttctgatc tgccgccgac agatcgatat
    18601 ccatagtgct gtcgcccatc gtcagttcaa aagtaccggt agcggaaaag gcgtcgtcgc
    18661 ccatactgaa cgtggtttgc tgcccctgcg ccagttgctc aacgaaaaac gagtagcttc
    18721 ccgcctgcgc ctgggaattg gcggaaacgg tcgccgagtc attattgctg gtggcggcaa
    18781 aagtcaccgg gccgtcggta tcgctgttca gcgcgtcaac ggcgctctgg aaatcgctca
    18841 acgccgagct gagcgagtcc aggccgctgc tttcagcatc cagctcactc tgcttctcct
    18901 gcaaattcgt cgcctgagta gcgacatccg catacgcgat ttcttgcgca atggttcttg
    18961 ggttgatcat tgatgactcc ggataacaat gtcaggttat tggcaaaagg cgtgccaata
    19021 ttttttatgt gaataatcaa tagattgaaa aacaggcgga aatgacgttt ccggtctggc
    19081 ggaaaaaagc ctgccgcaga aagcacaaca ccgcccacag gcggtgttgt tcgtggccgg
    19141 ataaggcgtt tacgccgcat ccggctatcg gtgcacagtg cctgatgcaa cgctgacgcg
    19201 tcttatcagg cctacgagtc cgcgcctgtt tttatgcgtg tgcccatttg taggccggat
    19261 aaggcgttca cgccgcatcc ggcaatcggt gcacaacgcc tgatgcgacg ctgacgcgtc
    19321 ttatcaggcc tacgagtccg cacatatttt tatgcttgtg cccgtttgtc ggcccgaact
    19381 tactgcagca ggctggaaac catgctggac atgctgttgg actgtttcag catggtgata
    19441 ccggtctgca tcagcacctg ttgtttggtc atattggacg cttccgtcgc gtaatcggta
    19501 tccatgatgt tgccgatagc cacttcggtg ttgtcctgca tgctggtcag gttgttggcg
    19561 gtgtcattca gacggttgat agatgcaccc aacttggact gaatctgaga aacgtcatcc
    19621 atcgcggtag aaatgctggt gatcatctgg ttagccgagc cagacgcagt cagttcagta
    19681 ccgccagaaa cgcctgcggt gccagtggtg tcagcctgat ccgcactaaa ggagttgctg
    19741 atagcagaaa ggtcagtcac cagcgtgttc agttggcttg aaatgttcac ggtcatggtg
    19801 tcggaagatt ccgcgcccac ctggaaagtt accgcgctct ggaacagacc gtcagtaccg
    19861 gtgttgctgg tgccggaaac accgaacagg ttggtgccgc cgtaagtagt gttttgcagc
    19921 atgtcagaca tctgctggcc cagttcgtcg tattcgtcct gcatcgcctg caggtcgtcg
    19981 tcgctgtagg tgccgttcgc tgcctgcgtt gacaggtctt tcatacgccc cagcacgtca
    20041 gacatttcgt cgaacatgct gtcagcggtt tgcagcatcg cggtagcatc attgatgttg
    20101 ctcagcgcca cacccatacc gctggactgt gccgtcagac ggttagcaat ttgtttgcct
    20161 gcggcgtcat cggcagaaga gttaatccgg ttacctgtcg ccaggcgttc catagaagta
    20221 gacagagaag agctgctttt gctgattgca ttaacggcgg ccattgaggc gttattggtg
    20281 ttaatagata acatgatact gctccttggg attaatcgtg ttcgtttcga taccctgtaa
    20341 aaacgacacc tcgggagcaa acttaaaatc gccgttaaaa ttttttcgcc acgtattatc
    20401 cgatgccact ttt
//
LOCUS       DACADF010000081        19612 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.082, whole genome shotgun sequence.
ACCESSION   DACADF010000081 DACADF010000000
VERSION     DACADF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.082"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            148..690
                     /locus_tag="ISB30_002854"
     CDS             148..690
                     /locus_tag="ISB30_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990466.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            790..1992
                     /locus_tag="ISB30_002855"
     CDS             790..1992
                     /locus_tag="ISB30_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAP1990467.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEEQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2212..2994
                     /locus_tag="ISB30_002856"
     CDS             2212..2994
                     /locus_tag="ISB30_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP1990468.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWERATPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3009..4214
                     /gene="rhmD"
                     /locus_tag="ISB30_002857"
     CDS             3009..4214
                     /gene="rhmD"
                     /locus_tag="ISB30_002857"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HAP1990469.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYREYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4271..5560
                     /locus_tag="ISB30_002858"
     CDS             4271..5560
                     /locus_tag="ISB30_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1990470.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPGQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5578..6381
                     /gene="yfaU"
                     /locus_tag="ISB30_002859"
     CDS             5578..6381
                     /gene="yfaU"
                     /locus_tag="ISB30_002859"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HAP1990471.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(6422..6643)
                     /locus_tag="ISB30_002860"
     CDS             complement(6422..6643)
                     /locus_tag="ISB30_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990472.1"
                     /translation="MTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQEGMHEQAIKIA
                     LRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(6656..7651)
                     /locus_tag="ISB30_002861"
     CDS             complement(6656..7651)
                     /locus_tag="ISB30_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAP1990473.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGEASPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRDFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQ
                     QGKLEGLQKGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLAATQLTDADIPNCH"
     gene            complement(7844..9034)
                     /gene="glpC"
                     /locus_tag="ISB30_002862"
     CDS             complement(7844..9034)
                     /gene="glpC"
                     /locus_tag="ISB30_002862"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAP1990474.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMDTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9031..10290)
                     /gene="glpB"
                     /locus_tag="ISB30_002863"
     CDS             complement(9031..10290)
                     /gene="glpB"
                     /locus_tag="ISB30_002863"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAP1990475.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10280..11908)
                     /gene="glpA"
                     /locus_tag="ISB30_002864"
     CDS             complement(10280..11908)
                     /gene="glpA"
                     /locus_tag="ISB30_002864"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAP1990476.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGAAVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12181..13539
                     /gene="glpT"
                     /locus_tag="ISB30_002865"
     CDS             12181..13539
                     /gene="glpT"
                     /locus_tag="ISB30_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAP1990477.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13544..14620
                     /gene="glpQ"
                     /locus_tag="ISB30_002866"
     CDS             13544..14620
                     /gene="glpQ"
                     /locus_tag="ISB30_002866"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP1990478.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDVL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            14823..15473
                     /gene="inaA"
                     /locus_tag="ISB30_002867"
     CDS             14823..15473
                     /gene="inaA"
                     /locus_tag="ISB30_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAP1990479.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15527..15781)
                     /gene="yfaE"
                     /locus_tag="ISB30_002868"
     CDS             complement(15527..15781)
                     /gene="yfaE"
                     /locus_tag="ISB30_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAP1990480.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(15781..16911)
                     /gene="nrdB"
                     /locus_tag="ISB30_002869"
     CDS             complement(15781..16911)
                     /gene="nrdB"
                     /locus_tag="ISB30_002869"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAP1990481.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(17000..19285)
                     /gene="nrdA"
                     /locus_tag="ISB30_002870"
     CDS             complement(17000..19285)
                     /gene="nrdA"
                     /locus_tag="ISB30_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAP1990482.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4511 a         4860 c         5326 g         4915 t
ORIGIN      
        1 ttattttttc ggcgtttaat tatttaagaa gtatttagtt acaataagtg actagatccc
       61 ttttttcgct cattgaatcc tctgcgccag gcagtacaat tccgcagctt tgggaatttt
      121 gattaattgc ttgtgggaaa gaggataatg aaaaaaattg cgctagcagg tctggccgga
      181 atgctgttgg tttctgcatc ggtcaatgca atgagcatca gcggccaggc gggtaaagaa
      241 tacaccaata ttggtgtcgg ttttgggact gaatcgacgg gcctggcgtt aagcggtaac
      301 tggacacata acgacgacga cggtgatgcc gcgggcgtgg ggctggggtt gaatctgcct
      361 ctcgggccat taatggcgac cgttggcgga aaaggcgttt acaccaaccc gaacgatggt
      421 gatgaaggtt atgcagcggc agttggcggt ggtttgcagt ggcaaattgg tgacagcttc
      481 cgcttgtttg gtgagtatta ctactctccg gattcgctct ccagcggaat taaaagttat
      541 gaagaagcga atgctggcgc gcgttacacc attatgcgtc cagtcagcat tgaggctggc
      601 taccgttacc tgaacctggc aggtaaaaat ggcaatcgtg ataacgccat tgctgatggt
      661 ccgtatgttg gggtaaatgc cagcttctga cgccttccgg cgcggcgttc tgtcgcgcca
      721 ttcagtttct tactacgccc tgtctccctt tgcgttatag tgattcgata acaaaagcag
      781 gagataacca tgttaaaagt ggaaatgtta tccaccgggg atgaagtgtt acacgggcaa
      841 atcgttgaca ctaacgctgc ctggctggcc gattttttct ttcatcaggg gctgccatta
      901 tctcgccgca atacggtggg ggataacctt gatgacttag tcaccattct tcgcgaacgt
      961 agtcagcacg ccgatgtgtt gatcgttaac ggcgggctgg gaccgaccag tgatgattta
     1021 agcgcactcg ccgctgcgac agcaaaaggt gaaggcctgg tgctgcatga agcctggctc
     1081 aaagagatgg aacgctattt ccatgaacgg ggacgagtaa tggcaccgag caaccgtaaa
     1141 caagcggagc tgcctgccag tgctgaattt atcaataacc cggtaggcac tgcctgtggt
     1201 tttgccatac agcttaatcg ttgcctgatg ttctttaccc ccggcgtacc gtcagaattt
     1261 aaggtgatgg tcgagcacga aatcctgccg cgcctgcgcg agcgtttttc tttaccacag
     1321 ccgccggttt gcctgcgttt gactaccttt ggtcgttcgg aaagcgatct ggcacaaagc
     1381 ctggacactc tacaactgcc gccgggcgta acaatgggct atcgctcctc aatgccgatt
     1441 atcgaactga aactcaccgg accggcaagc gaggaacagg cgatggaaaa actgtggctg
     1501 gacgttaaac gtgttgccgg acagagcgtg attttcgaag gcaccgaagg actgcccgcg
     1561 cagatcagtc gcgaattgca gagccgccag ttcagcctga cgctgagcga gcaattcacc
     1621 ggtggtttat tggctttgca actttctcgc gcaggtgctc cattgctggc gtgtgaagtg
     1681 gttccttcac aggaggaaac cctggcgcaa actgcgcact ggattacaga acggcgggcg
     1741 aaccattttg ctgggttggc actggctgtt tctggtctgg aagatgagca tctcaacttt
     1801 gcactagcca cgccagacgg cactttcgct ttgcgtgtac atttcagtgc cacacgctac
     1861 agtctggcta ttcgccagga agtgtgcgca atgatggcgc ttaatatgct acgccgttgg
     1921 ttaaacggcc aggacatcgc cagcgagcat ggctggattg aggttgttga gtccatgacc
     1981 ttatctgtct gattccgcca ctctgtgccg gtttactgca ccggcacatt ccccctccgg
     2041 caaaatttgt ccaacctgtt gtactcaata agaaaaaatc cgtgcgctga ttcacaaatt
     2101 tgtgcgtaat ttctccagtc cgctggacga gaaacgggat ttgatgcgct tcacaatttt
     2161 actccccctg aacaacgaga ctgattccag tatctaagac tggagtccag tatgttggaa
     2221 tcaagcaaag ttcccgccct gacacgggct attgatattc tcaatttgat tgcccggatt
     2281 ggcccctgta gtgctgcgac gatcattgac acattgggga tccctaaaag cacagcctat
     2341 ttgctgctta atgaactcag gcgtcagcgt tttcttagcc ttgatcacca ggaaaatttt
     2401 tgtttgtgga ccaggctggt ggagctttcc ggtcatgcgt taagcaagat ggatctccgg
     2461 gaactggctc gaccgcgttt gacgcaactg atggatacca ccgggctgtt atgtcacctg
     2521 gggattattg ataacggcag cgcttattac attttgaaag tggagtcatc agccactatc
     2581 agcgtgcgtt cccatgaagg aaaaagcctt tcgctttatc gctccggaat aggtaaatgc
     2641 ctgcttgcct ggcaacctgc agctgtacag caaagtatta tcgaagggct ggtctgggaa
     2701 cgggcgacac ccacaacgat tactcatcca caacggctct acgaagaact ggcgcgtatt
     2761 cgccgccagg gctggagcta tgacaacggc gaagattacg ccgatgtccg ttgtgtcgct
     2821 gcgccggtgt ttaacgccaa taacgagctt accgctgcga tttcggtggt cggtacccgc
     2881 ttacaaatca atgaagaata ccgtgattat ctggcgggca aggccattgc ctgtgccagg
     2941 gatatttcac gtttgttggg atggaaaagt ccctttgact tacaggcctc ataacaatgg
     3001 agaacatcat gaccctacca aaaatcaaac aggttcgcgc ctggtttact ggcggtgcga
     3061 cagcagaaaa aggcgctggc ggcggtgatt atcacgacca gggggcgaat cactggattg
     3121 atgatcatat tgccaccccg atgagtaaat accgcgaata cgagcagtcc cgccagtcat
     3181 ttggcattaa cgttcttggc acgttgattg ttgaagttga agcagaaaac ggccagaccg
     3241 gattcgctgt ttcgacagcc ggtgaaatgg gctgttttat tgtcgaaaaa catcttaacc
     3301 gtttcattga gggtaaatgc gtcagtgata tcaaactgat ccacgatcaa atgctcaatg
     3361 ccaccctgta ttactccggc tctggtggcc tggtgatgaa tacgatttcc tgtgtcgatc
     3421 tggctctgtg ggatctgttc ggcaaagtgg tcgggcttcc ggtttataaa cttttaggcg
     3481 gcgctgttcg tgatgagatt cagttctacg ccacaggtgc gcgtccggat ctggcaaaag
     3541 agatgggctt tatcggtggc aaaatgccga cgcactgggg gccacatgat ggcgatgcgg
     3601 ggatccgcaa agatgccgct atggtcgcgg atatgcgtga aaaatgcggt gaggatttct
     3661 ggttaatgct cgactgctgg atgagtcagg acgtgaatta tgccaccaaa ctggcccacg
     3721 cttgcgcgcc ctataacctg aaatggatcg aagagtgcct gccgccacag cagtatgaag
     3781 gttatcgcga actgaaacac aacgcaccag cggggatgat ggtcaccagc ggtgagcacc
     3841 acggcacact gcaatctttc cgtacgcttt cagaaaccgg tatcgacatt atgcagccgg
     3901 atgttggctg gtgcggtggc ttaaccacgc tggtggaaat tgccgcaatc gccaaatcca
     3961 gggggcaact ggtggtgcca cacggttcgt ctgtttactc ccaccatgcg gtgatcacct
     4021 tcaccaatac gccattcagc gaattcctga tgaccagccc ggattgttca acgatgcgtc
     4081 cgcaatttga tccgattctg cttaatgagc cagtaccggt gaatggtcgt attcataaat
     4141 cagtgcttga taaacccggt ttcggcgtcg aactcaatcg tgactgcaat ttgaaacgcc
     4201 cctacagcca ctaatcactc cgggcgttgc tgtgacaacg gtaacgccca catattctaa
     4261 ggagcttttt atgagcaccg ctttgcttga cgccgtggtg aagaaaaacc gcgcgcgttt
     4321 aattccgttt atgttggcgc tgtatgtgct ggcgtttctc gaccgttcga atattggttt
     4381 tgccaaacag acctaccaga ttgataccgg gctgagtaat gaagcttatg cgctgggagc
     4441 aggcattttc tttgtggtat acgcgtttct gggggttccg gcgaatcttt tgatgcgcaa
     4501 actgggggcc agaacctgga ttggtacgac aacactgctg tggggatttc tttcggctgc
     4561 catggcatgg gccgatactg aagcgaaatt tctgatcgtt cgcactctgc ttggtgctgc
     4621 ggaggccggg tttttccctg gtatgattta tctcacttcg caatggtttc cgcagcgtaa
     4681 tcgcgccagc attatggggc tgttctatat gggcgcaccg ctggcgttaa cactgggatc
     4741 accgctttcc ggcgcgctgc tggagatgca tggatttatg gggcatcccg gctggttctg
     4801 gatgtttgtg attgaaggat tgctggcagt cggcgctggg gtattcacat tcttttggct
     4861 tgatgacaca ccggggcagg cacgttttct gagtaaacaa gaaaaaacgt tgcttatcaa
     4921 tcaactggca agtgaagaac aacagaaagt gacttctcga ttgagcgatg cgctgcgtaa
     4981 tgggcgagtc tggcaactgg cgattatcta cctgaccatt caagtggcgg tttacggatt
     5041 aattttcttc ctgccgaccc aggttgctgc attgctgggg acaaaagtgg gctttacggc
     5101 gtcggtggtc accgccattc cgtgggttgc ggccttgttt gggacctggc ttattccgcg
     5161 ctactccgat aaaaccggcg aacggcgtaa tgtcgcagcg ctgacattac tggcggcggg
     5221 cattggtatt ggtctctccg ggctgctttc tccagtactg gcgatcgtag cactgtgtgt
     5281 tgcagccatc gggtttattg ccgtgcaacc ggtgttctgg acgatgccga cacaacttct
     5341 ttccggtacg gcgctggctg cggggattgg ttttgtaaac ctgtttggtg cagtgggcgg
     5401 gtttattgcc ccgatcctgc gcgtgaaagc agaaacgtta ttttccagtg atgcggcggg
     5461 attactgacg ctggcagcgg tggcggtcat cggttcgctg attattttca ctctgcgtgt
     5521 aaatcgcact gttgcgcaga ccgacgtggc acatcattaa atagtttaag gaacacgatg
     5581 aacgcattat taaccaatcc ctttaaagaa cgtttacgca agggcgaagt gcaaattggt
     5641 ctgtggttaa gctcaacgac tgcctatatg gcagaaattg ccgccacttc cggttatgac
     5701 tggttgctga ttgacgggga gcatgcgcca aacaccattc aggatcttta tcaccagcta
     5761 caggcggtag cgccctatgc cagccaaccc gtgatccgtc cggtggaagg cagtaagccg
     5821 ctgattaaac aagtcctgga tattggcgcg caaactctac tgatcccgat ggtcgatact
     5881 gccgatcagg cacgtcaggt ggtgtctgcc acgcgctatc ctccctacgg tgagcgtggt
     5941 gttggggcca gtgtggcacg ggctgcgcga tggggacgca ttgagaatta catggcgcaa
     6001 gtcaacgatt cgctttgtct gttggtgcag gtggaaagta aaacggcact ggataacctg
     6061 gacgaaatcc tcgatgtcga agggattgat ggcgtgttta ttggacctgc ggatctttct
     6121 gcgtcgttgg gctacccgga taacgccggg cacccggaag tgcagcgaat tattgaaacc
     6181 agcattcggc ggatccgtgc tgcgggtaaa gcggctggtt ttctggctgt ggctcctgat
     6241 atggcgcagc aatgcctggc gtggggagcg aactttgtcg ctgttggcgt tgacacgatg
     6301 ctctacagcg atgccctgga tcaacgactg gcgatgttta aatcaggcaa aaatgggcca
     6361 cgcgtaaaag gtagttattg atatcaaagg cccatgggga tcggctgtgg gcctgtgtta
     6421 attagtggtt attcgctgcc agatcggctt cgcttagctg agtggccgcg agcacctgat
     6481 cacgatcaat gccctgttcc agcatgcgca aagcaatttt aatggcttgt tcatgcatgc
     6541 cttcctgcaa accttctaat ttaccttctt gccagccttc aatcttaccc tcctgccagc
     6601 caatttgatg tccttcctgc cgtagccgtt cagcaatagt catcaatctc tccttttaat
     6661 gacagttcgg aatatcagca tcagtgagtt gggttgccgc cagcacaatc tcacgttcaa
     6721 agccctgctc cagcatgcgc aaggcaattt taatggcttg ctcatgcatg ccttcctgca
     6781 aaccttctaa tttacctttc tgcaaaccct ccaatttacc ttgctgcaaa ccttctaatt
     6841 taccttcttg ccagccttca attttacctt cttgccagcc aatttgatgt ccttcctgcc
     6901 gtagccgttc agcaatagtc attaatctct ccttttgtag tggtgaacgt tcggcaatct
     6961 cctgaataaa gcgggtgaaa cgggaggtat cgccgcattg cagcagataa ttaaacagtg
     7021 tttgtagctg gctgtcatta gtgaagtctc taaccaaaag cgtggtgatc ctgtcgacca
     7081 taccgattaa atcgcggtcg cgaatatgct tttgaatcag ttccagcaga gctatacgcc
     7141 gatgttgcat gatctcatcg tcaggtacga tggtgatatc caccaacgga aaagcttcgg
     7201 tgtacaactg ccgggcaagt tgcggatcgt caaactcatc cagccagttg agtgagtacg
     7261 ggtagggcga ggcttcgcca tgataaaaca gcaatggcac caccagcgga actctgtcat
     7321 agcctttatc caggtgacgc tgcatggcgg cagtggcata gcgcattagc cgaaaagcca
     7381 tattcttttc tgcagagctt tgatgttcaa tcacgcagta gatatagccg tcaccctcgc
     7441 tggtttccac ggaccacaaa acatccgagt agtaagcgcg taaacttttt tcaataaaac
     7501 tggtgggttc caggcgtaag gtttgcaggt tgcaaagctt gcgcagcggc tctggtagat
     7561 gtatttccag aaaatcccgt gcggtttcgg gtgtgaacat aaaggtttta aacaccgcat
     7621 catgcgggga ggaggttgtt gattcggtca tggttgtccg tcacctgata tttatgatga
     7681 cgcgacatta cggttatccc aggcctttga tatctgccag tttcagcctt tgcgagacgt
     7741 tttcagagga attttcactg cgttgcaacg catacattgg gcacggaatc gtgtacgcaa
     7801 aaatgcccgg taacaacatg cttaccgggc atcagaaagg agtttaagcc aacgcctgtg
     7861 ccagcagcgt aatcggatgt tcgcagcgaa gacttgtgga catctcaatc tgccatttgc
     7921 aggtttcgca gtcggtgacc accagatctg cgccgctctc ttctatctgg cggaacagtg
     7981 gtgcgccgat ggcttgtgaa gtggggtagt tctctttttt gaaaccgtaa gttcccgcaa
     8041 taccgcagca ctgggaatcc agcaccgtta actcaagccc cgggatttta cgcaacagct
     8101 ccagggtgta gagcgtccag cccatttttt ccatatggca cggagtgtga taaaccactt
     8161 tcagcggcag cggtttcagc ggtaacgttt tgccttcgtc cagcttgcgc cacagccagc
     8221 gggtcgccag ttcgatatga tcgcgcaagc ctttgttgtc gacattcagc acttctgggt
     8281 attcgtcgcg cagggcaaat gtacacgttg aggaggtggc aatcaccgga atgcctttta
     8341 ctcccacagc ttcgcggatc gactctacat tcgtaattgc ctgtttgcgt gctttatcgg
     8401 taaaaccgtt ggcgatcagc ggtacgccgc agcatttttc tttactgagc agttgtacgc
     8461 cggtatccat cgcgttgagc actttaatta aatctttgcc tagttgcgga tggttgtagt
     8521 taacgaagca gccgtgaaag aaagcgacct ggtctttata ctgcgcttgc tgagccgcca
     8581 cgctgcgata ccagcgacgg aacgtgccga aggagtattt cggtagcgtg cggcgatgat
     8641 cgatttttaa cgccgcatca agcagctgac gcaccggttt cagcgaggta gcggtgttga
     8701 cgatgggtgc gaacggcgtg gatacgctac ccatcaggtc ggtatgactc aacacaaaat
     8761 tacgcagcga cgggcgcgtg gtgtcatatt tcgcccgcgc gcgctggata atatcgccaa
     8821 tcttcacatc ggacgggcag gcgacttcac aacgtttgca gttgatgcaa tatttcagcg
     8881 cctcgtcata cagtgcgcca tctttcaaac gcagacgctc gccatccggc ccggcttgtt
     8941 ttggccccgg ataacccgga ttcacccggc tcaccgggca ggcggtggtg cagacggtgc
     9001 acttaatgca gttttcgaag ctggtgtcgt tcattgttgg cctcctgcgc gttgggcaat
     9061 ctgttgagcg gcatgtaaag cactgacggc acaaacaccg ccgccgcatc cctgggcgat
     9121 gggatcaaat ccgcccagca ccgaaccgat ggcaaacagg ttctcaatgg tttgccctgc
     9181 ctgcgacggg cgtagtgcct catcagtggt tacaccgaac tgctgccacg gctgcggcgc
     9241 aaaaaaatcg cccttatacc attcaccccg cgtagcggtt tgtagcacat caaggccgag
     9301 aatcggctcg cgaatgccgt cacgttccgc taccagtccg ccgctaaaga aactgccgct
     9361 cgcaagcacc gcaaatcgtg ggcgcagcgg gatatcggcg tgattgcgcg tccagatttc
     9421 attcactaca ccatttttac aggtcacttt tttcacttca tcgcccggca tccacacgcc
     9481 gccctggcgc acaaactggc gctgtaactg gttttgcaga cgaatgccca gcacggaagg
     9541 cggcagcgtt ggcaaaagca tcaatgaaca aggtagtttt tcattcaacc aatgccacag
     9601 tttgtcatcg gccaggccga agcaggcggg catcaggatc atttcgcagg tattggcgac
     9661 cggaataagc gcatcaagta acaacggcca gttttcttca ttatcaagga agcgggcaat
     9721 attcaccgcc cggaactcgg tggcgttatt gcgcagcaca tccagttccg gcagttcaat
     9781 ttcagcggtt tcaactttaa gatccaattc gcgcaatgaa gccgccgcaa gatgcgcctg
     9841 aaaatccatc aggccgctaa ttcccactac acatattttc ttcgcgggca gcggccagac
     9901 agggacttct ggcgaactta gccaggttga gcgcagagtg ccgagcggcg taatccgctg
     9961 gtgagccagt tctacgctgc cctgcaattg cgcaccgctt tcagcgatta atgcctgcgc
    10021 ctggcaagcg agatcgagca cgcgttgagg cccgagaaga gtgtaaggat gggcaggtgc
    10081 ctgctgacgc aaagattcca gtccactgtg aatgtctgtc accggttgac catccggcag
    10141 atggctcagc aaatccagcg atccagatga gaaatggagc gcgctttgac cacgagtgac
    10201 aatggcacag cgcaggccgt gtttttgcag ttgcaggcca cagagtaatc cggcgaggcc
    10261 gccgcccata atgacagtat caaagcgcat ctttctgctc cttctccaga ccacacaatc
    10321 cctgataaac ccagcgggta aattcgcttt cgcgcagtgc atctccccag gcgatgggtt
    10381 gcacgccttt ccagcgttcg tttaggaagg tggaaagttg ctcaatagat tgcgcggacg
    10441 tcgtgacgtt aaaacgttgc agcagtccgg cagcgcggca ggcgcagagc tcgccctggc
    10501 aggtgcccat ccccacacgg gtacgacggc gtaaatccag caggctatta acgtttaaat
    10561 tttctaccgc gtactgcact tcacccgcag ttaccgcttc gcactcacat accaggctac
    10621 ggtgcagacg gccttcgctc agccaggcag gcgtgcgatc gccatgacga taaaccgcag
    10681 aaccgcgcag cggggcaggt agggagatga ctttacgcaa ggtaacttca gcgggttcct
    10741 gtgaaccagg aagtgccaga tcggcggtcg tacaggggcg cgtgttgccc agtttgcggc
    10801 atacggcatc ggtagcccat tccgccatca gccgataggt catcagtttg ccaccggtga
    10861 tggtgataaa cccgtccaga ccatcgcgtt cagcatggtc gagcagcacg atgccacggc
    10921 tgacgttacg tccgctcgga tcgtcatcgc tggcaaccag cgggcgtacg ccagagtagg
    10981 cccgcaaaat gcgcgttttc gccatcacgg gggccagttt ttccccttca cgcagcagaa
    11041 tatcaacttc ttctgccgtt actcgattat cgtcaatctc gttgtaatca atacgtaaag
    11101 aggtggtgcc aatcagcgaa atggtatcgc caggcaccag aatatcggcg tcggaaggtt
    11161 tgcggcagcg gttgatcaca tgctgattaa tgcggtgatc catgatcagc agcgatcctt
    11221 tcgccgggaa catgcgaatg cgcagatcgg catattcggc aatgtgttgc ccccagatcc
    11281 cggcggcatt aactacgaca ggtgcatgaa gtgcctgaat ttcgccggtg agatggttac
    11341 gtacacgaac gccgcacacc gccgcgcctt cacgaatcag ccccgtgact tcatgagcgg
    11401 taaggataac ggcaccgtgt tctttggcat ccagcatgtt tgctgctgtc agacgaaatg
    11461 gatcaacggt gccatccgga actttcaccg cgccaatcag tgccgggtta acggcaggtt
    11521 cgataatgcg cgcttgctgc ggatcaatgg cttctgcgct gatccctgct tcttcgcagg
    11581 cgcggataaa agtggcctgg aaggagaggt catcttccgg cagggtgata aacaggccgt
    11641 tggttggttc tacgcagtga cgtgcaatgc gtttcaggat ctggttttca ctaatacatt
    11701 cgcgggccga ttccgcatcg gttaccgcat agcgcgcacc gctgtgcagc aggccgtggt
    11761 tacgcccggt ggcaccggtt gcgatgtcgt ggcgctcaac caaaatcacg cgcagcccgc
    11821 gcagggcaca gtcgcgggca atcccggctc ccgttgcgcc accgccaatg ataatcacgt
    11881 cacttgattg cgagtcgcga gttttcattg ttatccctca gaagttcgtt ttttaccatt
    11941 tagccatagt aaaaacatga attgtttgat ttcgcgcata ttcgctcata attcgaaagt
    12001 gaaacgtgat ttcatgcgtc attctgaaca tttcaaccat cgtatttaat aatgtgtgcg
    12061 gtaattcaca tttaatttat gaatgttttc ttaacatcgc ggcaactcaa gaaacggcag
    12121 gttctcccac tgaatcaggc tgttaatcat aaataagacc acgggccacg gaggctatca
    12181 atgttgagta tttttaaacc agcgccacac aaagcgcgct tacctgccgc ggagatcgat
    12241 ccgacttatc gtcgattgcg ctggcaaatt ttcctgggga tattctttgg ctatgctgct
    12301 tactatttgg ttcgtaagaa ctttgcgctc gctatgcctt atctggttga gcagggattc
    12361 tcacgcggtg atttaggttt tgccctttcg gggatctcga ttgcttatgg attttcgaaa
    12421 ttcatcatgg gttcggtatc ggatcgctcg aatccgcgcg ttttcctgcc cgcaggtttg
    12481 attctggcgg cggcagtgat gttgtttatg ggctttgtgc catgggcgac gtcgagcatt
    12541 gcggtgatgt ttgtactgtt gttcctctgc ggttggttcc aggggatggg gtggccgccg
    12601 tgtggtcgta ctatggtgca ctggtggtcg cagaaagaac gtggcggtat tgtgtcagtg
    12661 tggaactgtg cgcacaacgt cggtggtggt attccgccgc tgctgttcct gctggggatg
    12721 gcctggttca atgactggca cgcggcgctc tatatgcccg ctttctgcgc cattctggtg
    12781 gcactgttcg cctttgcgat gatgcgcgat accccgcaat cctgtggcct gccgccgatt
    12841 gaagagtaca aaaatgatta tccggacgac tataacgaaa aagcggaaca ggagctgacc
    12901 gcgaagcaga tcttcatgca gtacgtgctg ccgaacaaac tgctgtggta tatcgccatc
    12961 gccaacgtgt tcgtttatct gctgcgttac ggcatcctcg actggtcacc gacttatctg
    13021 aaagaggtta agcatttcgc gctggataaa tcctcctggg cctactttct ttatgaatat
    13081 gcaggtattc cgggcaccct gctgtgcggc tggatgtcgg ataaagtctt tcgtggtaac
    13141 cgtggggcaa ccggcgtctt ctttatgaca ctggtgacca tcgcgactat cgtttactgg
    13201 atgaacccgg caggtaaccc aaccgtcgat atgatttgta tgattgttat cggcttcctg
    13261 atctatggcc ctgtgatgct gatcggtcta catgcgctgg aactggcacc gaaaaaagcg
    13321 gcaggtacgg cagcgggttt taccgggctg tttggttacc tgggcggttc ggtggcggcg
    13381 agcgcgattg ttggctacac cgtggacttc ttcggctggg atggcggctt tatggtaatg
    13441 attggcggca gcattctggc ggttatcttg ttgattgttg tgatgattgg cgaaaaacgt
    13501 cgccatgaac aattactgca aaaacgcaac ggaggctaat ggcatgaaat tgacgctgaa
    13561 aaaccttagc atggcgatca tgatgagcac tatagtcatg ggaagcagtg caatggcggc
    13621 ggacagcaac gaaaaaatag tcatcgccca tcgcggtgcc agtggatatt tgccggagca
    13681 tacgctgcca gcaaaagcga tggcgtatgc gcagggagcg gattatctgg aacaggattt
    13741 ggtgatgacc aaagacgacc atctggttgt tctgcatgac cattatctcg atcgtgttac
    13801 tgatgttgcc gatcgtttcc cggatcgggc gcgcaaagac ggtcgttact acgcgataga
    13861 tttcacgctg gatgaaatta agtcgctgaa atttaccgaa ggtttcgata ttgaaaacgg
    13921 taaaaaagtg cagacttatc cggggcgttt cccaatgggt aaatctgact tccgggtgca
    13981 cacctttgaa gaagagattg aatttgttca ggggttaaac cactctaccg ggaaaaatat
    14041 cggtatctat ccagaaatta aagcgccgtg gttccatcat caggaaggga aggatattgc
    14101 ggcaaaaacg ctggaagtgc tgaagaaata tggttacacc ggtaaggacg acaaagttta
    14161 tttgcaatgt tttgatgctg atgagctgaa gcgtattaag aatgagctgg aacctaaaat
    14221 gggtatggat ctcaatctgg tacagctgat tgcctatacc gactggaatg aaacgcagca
    14281 gaaacagccg gacggaagct gggttaatta caattacgac tggatgttta agccgggtgc
    14341 catgaaacag gtggcggaat atgctgatgg tattggtccg gattaccata tgttgattga
    14401 ggagacatcg cagccgggta atatcaaact cactggcatg gtgcaagatg ctcagcagaa
    14461 taaactggta gtgcatcctt ataccgtgcg gtcagataaa ctgcctgaat acactactga
    14521 tgtgaatcag ttatatgatg ttctgtataa caaagcgggt gtaaatgggc tatttactga
    14581 cttccctgat aaggcagtaa aattccttaa taaagagtaa attaactacc ggatggcact
    14641 tatgtgctat ccgataccac tcttcattaa agtctgtggg ttatccttaa ttgcaattat
    14701 ttagagcgag atgacttgtt cccggcataa gatactcgtc aatacaacac gtattattaa
    14761 gattttcctc agctaaacat ctatagtcat tgtcgttaat aaaaaacgac aggcgatagg
    14821 gtatggcagt ttcagcaaaa cacgacgaat ttaatcactg gtgggcaaca gagggcgact
    14881 gggttgaaga gcctaactat cgccgtaatg gtatgagcgg ggtgcaatgc gtcgagcgca
    14941 acggcaaaaa gctgtatgta aagcgcatga cgcatcatct gtttcattcc gtacgttatc
    15001 cgttcggccg accaacgatt gtccgtgagg ttgcggtaat taaagaactg gaacgggccg
    15061 gtgtcatcgt gccgaagatt gtttttggtg aagcggtgaa aattgagggt gaatggcgcg
    15121 cgttgctggt gactgaagat atggcggggt tcatcagcat tgctgactgg tatgcccggc
    15181 atgcagtatc gccttattct gacgaagtac ggcaagccat gttgaaagca gtggcgctgg
    15241 cgtttaagaa aatgcatagc gttaatcgtc agcatggctg ttgttatgtt cgccatatct
    15301 atgtgaaaac agaaggtaag gcagaagctg gatttcttga cctggagaaa agtcgtcgcc
    15361 gcttgcgtcg tgataaagcg attaatcatg acttccgcca gctggaaaaa tatctggagc
    15421 cgatccccaa agcggactgg gagcaggtga aagcatatta ctacgctatg taatcgttgt
    15481 aggccagata agacgtataa gcgtcgtatc tggcgttaaa accaattcac atctcgattt
    15541 caatatcgcc ttttgcccgg caacaacagg gcaaaatttc ccccggctga ataaaagcta
    15601 acggttcggc aatccagtcc acctggcctg cgaccagtcg ggtgcgacag gagccgcagt
    15661 aaccttcgcg gcactggtac tcaaccgcca cattgtggga ttccagcgcc gccagaaggg
    15721 aagggtgttc atcctggcac agcagttgtg tgccagtaat gcgcagggta acgcgggcca
    15781 tcagagctgg aagttactca aatcgtcggt gtccacttcc gcgtcaatct gcccgaccag
    15841 ataagaactg acttccactt cctgcggagc aacctgcacg ttatcagaca ccagccaggt
    15901 gttgatccac gggatcgggt tggagcgcgt ctggaacggc agatccaaac cgactgcctg
    15961 catacggata ttggtgatgt attcaacgta ctggcagaga atgtctttat tcagaccaat
    16021 catcgaacca tcacggaaca gataatccgc ccagtctttc tcttgctgtg ccgcctggac
    16081 aaacaggtca tagcactcct gcttacattc ttcggcaatt tccgccatct ccggatcatc
    16141 cgcgccgctg cgcagcagat tcagcatatg ctgggtgccg gtcaggtgca gggcttcgtc
    16201 gcgggcaatc aggcgaataa ttttggcgtt accttccatc agctcacgtt cagcgaatgc
    16261 gaaagagcag gcaaagctga cgtagaagcg gatcgcttcc agcgcgttaa cgctcatcag
    16321 gcagagatac agttttttct tcagctcgcg caggctaacg gttacggttt taccgttaac
    16381 ggtgtgggtg ccttcgccca gcagatgcca gtagctggtc atttcgatca gttcatcgta
    16441 atagctggag atcccctccg cacgtttctg gatctgctcg ttggtaacga tatcgtcaaa
    16501 gacaacagac ggatcgttaa cgatattacg gatgatatgg gtataggagc gggaatgaat
    16561 cgtttctgag aacgcccagg tttctaccca ggtttccagt tccgggatcg agatcagcgg
    16621 cagcagtgcc acgttcggac tgcgaccctg aatggaatcc agcagcgtct gatatttcag
    16681 gttgctgata aagatgtgtt tttcgtgttc aggcagtgcc tggtaatcga tacggtcgcg
    16741 ggagacgtca acttcttccg gacgccagaa gaaagagagc tgcttttcga tcagcttttc
    16801 gaagatgtca tatttttgct gatcgtagcg agccacgttg accggctgac caaagaacat
    16861 tggttctttg agctgatcat ttttcgtctg tgaaaaggtg gtatatgcca tgaatgtgtc
    16921 ctgtggtttt attacccctc acccgtcccc tctcccgcaa ggggagaggg gacggtttgg
    16981 tgcaaagact gggggattat cagatcttac atgcgccgct ttcgcagcca tcgtcctgga
    17041 ttgacggcac cagatcgtcc tgcgcgtctt ctgcgccgtc acgggtgttc tgataataca
    17101 gcgttttgac cccgaatttg taggcagtga gcaggtcttt cagcaactgc tgcatcggca
    17161 cttttcctga cgggaaacgt gacggatcgt agttggtgtt agcagagatc gactgatcga
    17221 taaatttctg catgataccc accagttgca ggtagccatc gttacccggc atttcccaca
    17281 acagctcata ggcgtcgtgc agatgctcgt agtccggcac cacctggcgc agaataccgt
    17341 ctttcgacgc tttgatgctg acgtaaccgc gcggcggttc aataccgtta gtggcgttag
    17401 agatctgcga agaagtctcg gacggcatca gggcagaaag cgtggagtta cgcaggccgt
    17461 gcgttttgat tgactcacgc agtgcttccc agtcgtaatg cagcggctca ttagcgatgg
    17521 catccagatc tttcttatag gtatcgatcg gcaggatccc tttcgcgtat gtggtttcgt
    17581 taaaccacgg gcacgcgcct tgctctttcg ccagctcatt agacgctttc agcaggtaat
    17641 actgaatggc ttcgaaggtt ttatgcgtca ggttgttggc gctgccgtcg gagtagcgtt
    17701 taccgtgctt cgccaggtag taagcgaagt tgatcacacc aatacccagc gtacgacgac
    17761 ccatcgctcc acgtttggcg gccgggatcg ggtaatcctg ataatccagc agcgcgtcga
    17821 gtgcgcgtac tgccagaatt gccagctctt ccagttcatc caggctgtta attgcgccca
    17881 ggttgaaagc agacagcgta cacagtgcga tttcaccgtt ctcgtcgttg acgtcgttca
    17941 gcggtttggt cggcagggcg atttccaggc acaggttaga ctggcgcact ggcgcgatgg
    18001 ccggatcaaa cgggctatgg gtattgcagt ggtcaacgtt ctgaatatag atacgaccgg
    18061 tagacgcacg ttcctgcatc atcagcgaga acagctcaac ggctttcaca cgctgcttgc
    18121 ggatgctgtc gtctttctca tatttggtat acagacgttc aaactcttcc tgatcggcga
    18181 agaacgcgtc gtacagcccc ggtacgtcgg acgggctgaa cagagtgata tcttcacctt
    18241 tcagcagacg ggtatacatc agtttgttga tttgaacccc gtagtccata tgacgcacgc
    18301 ggttgccttc cacaccacgg ttgtttttca acaccagcag gctttccact tccagatgcc
    18361 acatcgggta gaacagcgtt gccgcaccgc cgcgcacacc gccctgagag caggatttca
    18421 ccgctgtctg gaaatgtttg tagaacggaa tacaaccggt atggaacgct tcgccaccgc
    18481 gaatcgggct acccagcgca cgaatacgcc cggcgttgat gccgatcccg gcacgctggg
    18541 aaacgtattt aacaatcgcg ctggaggtgg cgttaatgga atccaggctg tcgccgcact
    18601 cgatcagcac gcatgagctg aactgacgag tcggggtacg cacgccggac atgattggcg
    18661 tcggcagcga aattttaaat gtggaaaccg cgtcgtaaaa acgcttcacg tattgcaggc
    18721 gcgtttcacg cgggtagttc gagaacaggc acgcggcaac cagaatataa aggaactggg
    18781 cgctttcgta gatttcgccg gtcacgcggt tctgtaccag atatttgcct tccagctgct
    18841 taacggcagc ataagagaag gtcatatcac ggtcgtgatc gataaacgtg tccatctgct
    18901 tgaactcttc ttccgtgtag tcttccagca gatgattatc gtatttgccc atctcgacca
    18961 ttttcaccac atggtcgtac agcgcaggcg gctcaaactg accgtaggct tttttacgca
    19021 ggtggaagat cgccagacgt gcggcgagat actgataatc cggcgcatca cgggagatca
    19081 ggtctgcggc agccttgata atggtttcgt ggatgtcaga ggtcttgata ccgtcataaa
    19141 actgaatgtg ggagcgcagc tcgacctggg aaatcgaaac gttatgcagt ccttctgccg
    19201 cccaatccag aacgcgatgg attttgtcga gattgatgcg ctctgtgcta ccgtcgcgct
    19261 ttgtcaccag cagattctga ttcatgtatg tcgtacctgt ttttcaatag cttcatgcag
    19321 actacctgta gtgagggaag ctgctaagaa cactatatat agggggtatg tttgggattc
    19381 actgcaagat agtgtgaaac tggccctctt gcaagtgcat aactttgtgg ataactcagg
    19441 aaggaaaaag tggcttccgc gcaccttagg tcagacaagg tgtccgggaa agtcaatggg
    19501 aagagaaaaa tttgttaaaa ataaccgttc ggtgaattct tgaacggtaa aataggatag
    19561 gtaaattgat atagatggtt tattaatctt gttgctaatg attgccaaaa tt
//
LOCUS       DACADF010000082        19357 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.083, whole genome shotgun sequence.
ACCESSION   DACADF010000082 DACADF010000000
VERSION     DACADF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.083"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            296..3559
                     /locus_tag="ISB30_002871"
     CDS             296..3559
                     /locus_tag="ISB30_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP1990483.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYMNPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEASIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERNNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVKGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDYIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSSLN"
     gene            3774..4112
                     /gene="symE"
                     /locus_tag="ISB30_002872"
     CDS             3774..4112
                     /gene="symE"
                     /locus_tag="ISB30_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HAP1990484.1"
                     /translation="MTDTHSIALPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVMEGCIVLTAQPPAIEESELMQSLRQVCKLSVRKQRQVQEF
                     IGVISSKTPR"
     gene            complement(4155..4319)
                     /locus_tag="ISB30_002873"
     CDS             complement(4155..4319)
                     /locus_tag="ISB30_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HAP1990485.1"
                     /translation="MEFYENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            4496..5908
                     /locus_tag="ISB30_002874"
     CDS             4496..5908
                     /locus_tag="ISB30_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP1990486.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCGSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRTNCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(6151..7107)
                     /locus_tag="ISB30_002875"
     CDS             complement(6151..7107)
                     /locus_tag="ISB30_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAP1990487.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLEYASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPATARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLAVLLVTGCANDSQITALLNYIL
                     LTGDEARFNEFISELIRRMPQHRERIMTIAERIHNDGWLLGRERGRKEGKEEGEKSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKRDPWIEY"
     gene            complement(7104..7316)
                     /locus_tag="ISB30_002876"
     CDS             complement(7104..7316)
                     /locus_tag="ISB30_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990488.1"
                     /translation="MRLIKRVRWKGEYLPGTGNVKCEPVSGKMYRLQRNKNSLESTSG
                     NEKLPGENGFVSRYSRAILSTGDGSP"
     gene            7574..8806
                     /gene="mdtM"
                     /locus_tag="ISB30_002877"
     CDS             7574..8806
                     /gene="mdtM"
                     /locus_tag="ISB30_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HAP1990489.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATIALSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANTIVARFIK
                     DPTEPRFIWRAVPIQLAGLALLIVGNLLSPHVWQWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            8847..10127
                     /locus_tag="ISB30_002878"
     CDS             8847..10127
                     /locus_tag="ISB30_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HAP1990490.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARTDAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHHVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            10243..11394
                     /locus_tag="ISB30_002879"
     CDS             10243..11394
                     /locus_tag="ISB30_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HAP1990491.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLHKTVEERFGHE
                     ISEDALRDAIVLKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRHQHNIPYIAIETDYSTSDIGQLSTRVAAFIEML"
     gene            11404..12171
                     /locus_tag="ISB30_002880"
     CDS             11404..12171
                     /locus_tag="ISB30_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990492.1"
                     /translation="MAYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            12168..12425
                     /locus_tag="ISB30_002881"
     CDS             12168..12425
                     /locus_tag="ISB30_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HAP1990493.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            12490..13350
                     /locus_tag="ISB30_002882"
     CDS             12490..13350
                     /locus_tag="ISB30_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HAP1990494.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLAGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            13418..14596
                     /locus_tag="ISB30_002883"
     CDS             13418..14596
                     /locus_tag="ISB30_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1990495.1"
                     /translation="MPSPSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSMLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(14609..15163)
                     /gene="kptA"
                     /locus_tag="ISB30_002884"
     CDS             complement(14609..15163)
                     /gene="kptA"
                     /locus_tag="ISB30_002884"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAP1990496.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKVGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            15413..16096
                     /locus_tag="ISB30_002885"
     CDS             15413..16096
                     /locus_tag="ISB30_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990497.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            16093..16554
                     /locus_tag="ISB30_002886"
     CDS             16093..16554
                     /locus_tag="ISB30_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990498.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            16567..17739
                     /gene="iadA"
                     /locus_tag="ISB30_002887"
     CDS             16567..17739
                     /gene="iadA"
                     /locus_tag="ISB30_002887"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP1990499.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            17804..18715
                     /gene="hypT"
                     /locus_tag="ISB30_002888"
                     /gene_synonym="qseD"
     CDS             17804..18715
                     /gene="hypT"
                     /locus_tag="ISB30_002888"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAP1990500.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(18708..19100)
                     /gene="iraD"
                     /locus_tag="ISB30_002889"
     CDS             complement(18708..19100)
                     /gene="iraD"
                     /locus_tag="ISB30_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAP1990501.1"
                     /translation="MMRQSLQAVLPEISGNKTSPLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDESLTFDLVWDNGGWRSATLENVS"
     gene            complement(19097..19180)
                     /locus_tag="ISB30_002890"
     CDS             complement(19097..19180)
                     /locus_tag="ISB30_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAP1990502.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         4645 a         4857 c         4867 g         4988 t
ORIGIN      
        1 ttacctctaa ttattccatc aataaatata attgagaaat ttgatgaatt aacaagcaat
       61 ttcagagaga tgatgaataa ctcattaatt caaaacgaat atctcaccaa cctccgaggc
      121 accttgctcc cgaaactcat ctccggcgag ctatccctgg aagatcttcc ggatctcacc
      181 accgatacag aagccgcata acgcattttg cccctgtaaa atcaggggct ttctggtaag
      241 gttttctact gatacgggaa tgcttaccag aaattagcca gggttggagc gcgatatgag
      301 tctctctttc agtgaagcaa aattagaaca agcgatcatt gaactgttac aggatcaggg
      361 atatcaacac ctgatcggcg ataatgtccc acgttcgagc ctcgatcagg tcattatcga
      421 agacgatctc cgtcattatt tagcggcacg ctaccagcct gatggcatta ctgaagaaga
      481 gattcagcga ctgatcaaac agttcaccac gcttccggcc tccgatcttt atgaaagcaa
      541 caaaacattt tgtcgctggc tggcaaatgg ttttctgttc aaacgcgacg atcggcaaca
      601 aaaagatctc tatattgaat tgctcgacac ccggcatcta cctgccgcac tgcgccagat
      661 atttgacgcc gaagatgtcc cgttgcaaca ggcggcggaa ctcccgcctt cctatatgaa
      721 tccgccgctt aaccggatta agattgttaa ccagcttaaa atctccggca aagataatca
      781 gactcgtatt cctgacggca ttctctatat caatggtctg ccattggtcg tctttgaatt
      841 taaaagtgcg gtgcgcgagc aggaagccag tattggcgat gcctggaagc agctctgtaa
      901 acgctatcgc cgggatattc cgcaactgtt tatctacaac gcgctctgca ttattagcga
      961 tggcgtaaat aaccggatgg gcaacctgtt tgcgccctat gaatactttt actcatggcg
     1021 caaagtcacc ggtaatgaaa accgtgaaca ggacggaatt ccatcattgc actcaatgat
     1081 tcaggggctg tttcatccgg tacgtctgct ggatgtgatt aaaaacttta tctgcttccc
     1141 ggataaagcc aagcacgaag taaaaatttg ctgccgatat ccgcagtact atgccgcccg
     1201 caaactctat tacagcatca agcaagcgcg taaacctttc ggtagcggta aaggcggcac
     1261 ttactttggc gcaacgggct gtggtaaaag ttacaccatg caatttttaa cgcgtctttt
     1321 gatgaagagc gtggagtttg ccagcccgac cattgttttg atcaccgacc gcaccgatct
     1381 ggacgatcag ctttctgcgc aaatgtgcaa cgccaaaaat tacattggcg acgacaccat
     1441 cgttcccgtt accagccgtg aagatttgcg taatcaactg gcaggacgca atagtggcgg
     1501 agtctttctg acaacgatcc ataaattcac cgaagacacc gaactccttt ctgaacgcaa
     1561 caatatcatt tgcatctcgg acgaagcaca tcgtagccag gttaacctcg accagaaagt
     1621 catcgttgat aaagaaagcg gaaaagtgcg caaaacgtat ggctttgcga aatacctgca
     1681 cgattcactg ccaaacgcca cctatgttgg ctttaccggc acaccgattg acgcgacgct
     1741 cgatgtcttc ggtgaggtga tcgacagtta caccatgacc gaatccgttc aggatgaaat
     1801 cactgtacgc atcgtgtacg aaggccgtgc ggctaaagtg atcctggact ccagcaaact
     1861 ggaagaagtc gaaaagtatt acgaagagtg cgcaaacgca ggcaccaatg agtggcaaat
     1921 cgacgaaagc aaaaaagcca ccgccaccat gaatgcggtt ctgggtgatg aagatcggtt
     1981 aaaagccctc gcagaagatt ttgccaaaca ttatgaaaaa cgcgtagccg aaggttccac
     2041 cgtaaaaggc aaagccatgt ttgtttgtgc cagccgtgaa attgcctggg atttctaccg
     2101 tcagcttaaa gctattcgcc ctgcctggtt tgaagtgaag caagcacccg atggcgtcgt
     2161 cctgacggaa caggagcaga aagagttacc gccttcagaa atggtgaaga tggtcatgac
     2221 gcgcggtaaa gatgacgacg aggcgcttta tgatttactg ggcacaaaag aatatcgcaa
     2281 agagctggat aagcagttca aaaacgctaa atcgaatttc aaaattgcca ttgttgttga
     2341 tatgtggctg accggtttcg acgtcccgga actggatacc atctatattg ataaaccctt
     2401 acaaaaacat aatcttatcc agactatttc tcgcgttaac cgtaaactgg aaggcaaaag
     2461 taaagggtta gtggtggact acatcggcat taaaagtcag atgaaccagg cactggcaat
     2521 gtattcccgc attgatgcca ccaactttga agatattcag caatcggtaa tagaagttaa
     2581 aaaccatctc gatttgttgg ggcaagtctt ttacgaattt gacagtcggg attattttag
     2641 tggtgagcca caagcgcaat tatcctgcct caaccgcgcg gcggaatttg ttctgcgtac
     2701 ccagaaagtt gaacgtcgtt ttatgggact ggttaaacgc atgaaagccg cctacgacgt
     2761 ctgctgcggc agcgaagcat tatcacagac agaacgtgac tatattcact attatcttgc
     2821 tgttcgttca attgttttca aactgacgaa aggtgacgca ccggatgtta cccagatgaa
     2881 tgcacgcgtt cgtgaaatga ttgcagaagc gttaaaagct gatggcgtag aagaaattta
     2941 ttttcttggc gataaaaaag cggaatccat cgatattttt gacgaagatt atctggcgcg
     3001 aattaacaag atcaaacttc cggcaacgaa gatccagtta ttacaaaaat tactggaaaa
     3061 agcgatcagc gacttcaaga aagtgaacca gttgcaaggg attaacttca cccgccgctt
     3121 ccaggcgatt atagatcgtt ataatgagcg gcgagaagat gatgtactca acggtgaaga
     3181 attcgataca ttcagtcagg aaatgaccga tattatctat gatattaaaa ccgaaatggg
     3241 cacctgggcc gatttaggca ttgatattga agaaaaagcc tttttcgaca ttcttgctca
     3301 tatgcgcgat aaatatcagt tcacctatga cgatgaaaaa atgctgtcac tggcaaaaga
     3361 gatgaaaagc gtggttgaca acacatctaa atatcctgac tggagtaaac gcgatgatat
     3421 taaagcgaaa ctgaaagttg aacttattct gcttctacac aagcataagt tcccgccagt
     3481 agcgaatgat gatgtttata tgggtgtgct ggcacaggca gagaatttta aagaacacca
     3541 tatgagttct ttaaattaat tatgaaggcc ggatacactt tgttatccgg ccattctgaa
     3601 ttaacgttaa tttcccttcc agatctttcc agtaatgctt ttattatttt ctatttttag
     3661 ctggactttt cccatattta ctgatgatat atccaggtat ttagcgcgat gcggatgtgt
     3721 tggcgcacac ccgcaccgct catcacaatc actatttata gggaatagca gttatgactg
     3781 acacgcattc tattgcacta ccgtttgaag cagaagtctc cccggcaaat aaccgtcaat
     3841 taaccgtgag ttatgcgagc cgctacccgg attacagccg tattcccgcc atcaccctga
     3901 aaggtcagtg gctggaaacc gccggttttg ccaccggcac ggcggtagat gtcaaagtga
     3961 tggaaggctg tattgtcctc accgcccaac cacccgccat cgaagagagc gaactgatgc
     4021 agtcactgcg ccaggtgtgc aaactgtcgg tgcgtaaaca aaggcaggtg caggagttta
     4081 ttggggtgat tagtagcaaa acgccacgct aagtttctct ccccttcccc acacgggaag
     4141 gggaattttc cccctcactc cacatcctcc ctgcgtaacc caatatccct caaccgctca
     4201 tcactcatct gctgtaacac tcgtcgggtc tgcatttgcc gccgccaacg cctcaccgcc
     4261 tgccagagtt gcacaagtct gataaacggc gttttggctt tgttttcgta aaattccatc
     4321 tgctgcctcc tcatctttca gaggcttcat catccgtctt ctggcagttc acaatacaga
     4381 tgcaaatatt agttttattt aacatacaga cagattaaaa cagcatctgc atccggtttt
     4441 ttagcctcat ctgtaccggt ttttctctct gtatggtcca aataaggaat acagcatgac
     4501 gcgttatcaa catctggcga ctctacttgc cgagcggatt gagcaggggc tgtatcgtca
     4561 cggggagaaa ttgccgtcgg tgcgcagctt aagtcaggag cacggcgtca gcatcagcac
     4621 cgtgcagcag gcgtaccaga cgctggaaac gatgaagctc atcactccgc agccgcgttc
     4681 ggggtatttt gtcgcacaac gtaaagccca gccgccagtg ccgccgatga cgcgtccggt
     4741 gcagcgcccg gtggaaatta cccagtggga tcaggtactg gatatgctgg aggcgcatag
     4801 cgacagttcc attgttccgt taagcaaaag cacgccggat gtcgaaacgc ccagcctgaa
     4861 accgctgtgg cgggagctaa gccgggtggt gcagcataat ctgcaaaccg ttctcggtta
     4921 tgacttgtta gccggtcagc gagtgttgcg agagcagatt gcccgcctga tgctcgacag
     4981 cggctcggta gtcaccgccg atgacatcat cattaccagc ggctgccata actcgatgtc
     5041 gctggcgtta atggcggtgt gtaaaccggg cgatattgtc gcggtcgaat ccccctgtta
     5101 ttacggttcg atgcagatgc tgcgtggcat gggcgtgaaa gtgattgaaa tcccaaccga
     5161 tccagaaaca ggcatcagcg ttgaagcgct ggaactggcg ctggagcagt ggccgattaa
     5221 aggcatcatt ctggtgccaa actgtaataa tccgctggga tttattatgc cggacgcgcg
     5281 caaacgggcc gttctctctc tcgctcagcg tcatgatatt gtgatttttg aagatgatgt
     5341 ctacggtgaa ctggcaacgg agtatccgcg cccgcggacc attcattcct gggatatcga
     5401 cgggcgagtg ctgctgtgcg gctcgttcag taaaagtatt gcacctggcc tgcgcgtggg
     5461 ctgggtcgca ccggggcgct atcacgataa actgctgcat atgaaatatg ccatcagcag
     5521 ctttaatgtg ccgtccacgc aaatggcggc ggcaacgttt gtgctggaag gtcactatca
     5581 tcgccatatc cggcggatgc ggcagatcta tcagcgcaat ctggcacttt atacctgctg
     5641 gatacgggaa tattttccct gcgaaatctg tattacgcgc ccgaaaggcg gatttttact
     5701 gtggatagaa ttgcctgaac aggtcgatat ggtctgcgtc gcgcggcagc tgtgccgcat
     5761 gaaaatccag gtggcggcag gctcgatttt ctcggcttcc ggcaaatacc gtaattgtct
     5821 gcgcaccaac tgcgctttgc cgctcagcga aacctatcgc gaagcactga agcaaattgg
     5881 cgaggccgtg tatcgggcaa tggaataaat tcgacatagt ggcgaagcgc ctgatttact
     5941 ctcacgaata gagtgtgact cgttttatct ttgcagggta atcgccacgt tccagcatgg
     6001 cttaggcaaa gtgcgctttg ttcataccgg atgcgatgtg aacgccttac caggtctaca
     6061 aaatctttaa agttaaatat atttaatggg ttatgtagac ctgataagcg tagcgcatca
     6121 ggctgctttg cgtttgtcat cagtctctga ttagtactcg atccatggat cgcgcttgct
     6181 ttcaggcaag ggctgatcta atgcctgcat ttgctctgcc gaaagtccgg tatatcgttg
     6241 tatccattca ggatccgccc cattctgcaa caacaatcgg aggaggctct tttccccttc
     6301 ttctttccct tctttcctcc ccctctccct tcccaacagc catccatcat tatgaattcg
     6361 ctctgcaatc gtcattattc gctccctgtg ttgtggcatt cgacggataa gttcgctgat
     6421 aaactcatta aaacgcgctt catcgccagt cagtaaaatg taatttaaca gcgcggttat
     6481 ctggctgtca ttagcgcacc ctgtaaccag taatactgcc agttgatcaa taagccccat
     6541 tagatcgcgc tggcgaatat gcttttggat caactccagc agggcgactc tgcgatgctg
     6601 cacaatctcg tcgtctggca cgacagtgac atccaccagc gggaacgctg cggtataaag
     6661 cttccgtgcg gtagccgggt cggcaaattc atccagccag cacaaagacc aggggtaagg
     6721 actacggcta ccgtgataaa acagcatcgg gatgaccagc ggtagcggcc ggcgtttatc
     6781 atgctcgata tggcgctgca tcaccgccat ggaatatcgc atcaggcgaa aggccatatg
     6841 gatatcctcg cggctctgat gttcaatcac cacataaata tagccatctc cttcgcgggt
     6901 ctttaccgac cacagaatat cggaatgtag cgcccgcaat ttttcatcga caaagctggc
     6961 gtattccagt tttaagctgt cgagatcgca cagttcacgt aaatctttgg gtaagtggat
     7021 ctccataaaa tcccgcgcgg tgtcagggtg ggtgagaaaa gatttaaata atgcgtcatg
     7081 cggcgtgctg gtcgtgaagt ttgtcatggc gatccgtcac ctgtagataa gatggcccga
     7141 ctgtagcgac taacaaaacc attctcaccc ggcaatttct cgttccccga ggtgctttcc
     7201 aggctatttt tgttcctctg caaacgatac atttttccgg aaacaggctc gcattttaca
     7261 tttcctgtac ccggcagata ttcccctttc caccgcactc ttttgattaa ccgcaaattt
     7321 taattacact taaggtgtat attttctatg caacccatca attcaagagg tgtaatgtgc
     7381 tgatgactat ttgtaatcgt tatacattct gacctggagt cagaaggtat ttctctgtct
     7441 gtatgttcac aggcagtgtg gttgattaca tgaaattcag tacatttgcc cgtctcgcct
     7501 gcctttcgtc attaccgacg gtattgaatt tcgttttccc cattgggatt ctccggacaa
     7561 ggagttgttt gttatgccac gtttttttgc ccgccatgcc gccacgctgt ttttcccgat
     7621 ggcgttgatt ttgtatgact ttgctgcgta tctgtcgacg gacctgatcc agccagggat
     7681 cattaatgtg gtccgtgatt ttaatgccga tgtcagtctg gccccggctg ccgtcagtct
     7741 ctatctcgct ggaggcatgg cattgcagtg gctgctgggg ccgctttccg acaggattgg
     7801 ccgcaggccg gtgctgatta ccggggcgtt aatttttacc cttgcctgcg ccgcgacaat
     7861 gttcaccacg tcaatgacac aatttcttat cgcgcgtgca attcagggca ccagtatctg
     7921 ttttattgcc accgttggtt atgtcacggt gcaggaggcg ttcggacaga caaaagggat
     7981 caagttgatg gcgattatca cctccatcgt actgattgcg ccgattattg gcccgctttc
     8041 cggcgcagct ctgatgcact ttgtgcactg gaaagtcctt tttgccatca ttgcggttat
     8101 gggttttatc tcatttgtcg gcttgctgct ggcgatgcca gagacggtga agcgcggcgc
     8161 ggttccgttt agcgccaaaa gcgtcttgcg cgattttcgc aatgtctttt gcaaccggct
     8221 gttcctcttt ggcgcggcaa ccatcgccct cagctatatc ccaatgatga gctgggtggc
     8281 tgtctcgccg gtgatcctga tcgatgcggg cggcttaaca acttcgcagt tcgcctggac
     8341 gcaggttccg gtattcggcg cggtgattgt cgccaacacc atcgtggcgc gatttatcaa
     8401 agatccgacc gaaccgcggt ttatctggcg tgccgtgccc attcaactgg ccggcctcgc
     8461 actgttgatt gtcggcaatt tgctgtcgcc acacgtctgg cagtggtcgg tgctcggcac
     8521 cagtctgtat gctttcggga ttggtttaat cttcccgacc ttattccgct ttacgctgtt
     8581 ttccaataac ttaccgaaag ggaccgtctc cgcatcactg aatatggtga tcctgatggt
     8641 gatgtctgtc tcggtcgaaa tcggccgctg gctgtggttt aacggcggtc gcttgccgtt
     8701 tcatctgtta gccgtcgtag cgggcgttat cgtcgttttc accctggcgg gattgctcaa
     8761 tcgcgtgcgc cagcatcagg ctgccgagct ggcggaggag cggtgatttt tgcgcgatcc
     8821 tgccgtcagg ctctattctt aacgttatga ataaactcat tgaactcaga cgcgccaaaa
     8881 tgttggcgct ctctttactg cttatcgccg ctgctacctt tgtcgttacg ctgtttttgc
     8941 cacccaattt ttgggtgagc ggcgtgaagg cgattgctga agcggcgatg gtcggcgcgc
     9001 tggcggactg gtttgcggtg gtggcgctgt ttcgccgcgt gccgattccg atcatttctc
     9061 gtcatacggc gattatcccg cgtaataaag accggattgg cgaaaatctc ggtcagttcg
     9121 tgcaggaaaa atttctcgat acccaatcgc tggtggcatt gattcgacgt cacgaaccgg
     9181 cgttgttgat tggcaactgg tttagtcagc cagaaaacgc ccgccgcgtt ggtcagcatc
     9241 tgttgcagat catgagtggt tttcttgaac tgaccgatga tgcgcgtatt cagcgcctgc
     9301 ttaagcgcgc agtccatcgg gcgattgata aggtcgatct ttccggcacc agtgcgttga
     9361 tgctggagag tatgaccaaa aacgatcgtc atcaggtgct gctggatacg ctgatcgcac
     9421 agttgatcgc ccttctccag cgcgataaat cgcgcaagtt tatcgcccag cagattgttc
     9481 gctggctgga gagtgagcat ccactgaaag ccaaaatttt gcccactgaa tggctgggcg
     9541 aacatagcgc ggagttggtt tctgacgcgg tgaattcttt gcttgatgat attagtcgcg
     9601 atcgtgcgca tcagatccgc catgcgtttg atcgcgccac ctttgccctg atcgacaagc
     9661 tgaaaaacga tcctgaaatg gcagcgcgaa ccgatgccgt aaaaagctat ctgaaagaag
     9721 atgaagcttt taaccgctat ctcagtgaat tgtgggggga tttacgggaa tggctgaaag
     9781 cggatatcaa cagtgaagat tctcgtgtga aagaacgcat cgcacgagcg ggtcaatggt
     9841 ttggcgaaac gttaattgcc gatgatgcct tgcgggcgtc gttaaatggt catcttgaac
     9901 aagccgcgca ccacgtcgcg cctgagtttt ccgcattcct gacgcgccac atcagcgata
     9961 cggtaaaaag ctgggatgcg cgggatatgt cgcggcaaat agaattaaat atcggcaaag
    10021 atctgcagtt tattcgtgtt aacggtacgc tggttggcgg atgtattggg ctgattttgt
    10081 atttgctgtc gcagctcccg gctttgttcc ccctcggcaa tttttagaaa tccatgaaaa
    10141 actaagtggc taaccgcgag agagatcaaa taaaccgcac taaaagaatg gtaataatgt
    10201 ggcttctttt cattatctcc cgtggtacgg ggaaggaaaa tcatgtcact tgtcaccgat
    10261 ctacccgcca ttttcgatca gttctctgaa gctcgccaga aaggctttct caccgtcatg
    10321 gatctcaagg agcgcggcat tccgctggtt ggtacctact gtacttttat gccgcaagag
    10381 atcccaatgg cagccggtgc ggttgtggtt tcactctgtt ccacctctga tgaaaccatt
    10441 gaagaagcgg aaaaagacct gccgcgcaat ctctgcccgc tgattaaaag cagctacggc
    10501 ttcggcaaaa ccgataaatg cccctacttc tacttttctg atctggtggt cggtgaaacc
    10561 acctgcgacg gcaaaaagaa aatgtatgaa tacatggcgg aatttaagcc cgttcatgtg
    10621 atgcaactgc caaacagcgt taaggacgat gcctcacgcg cgttatggaa agccgagatg
    10681 ctgcgcttgc ataaaacggt agaagaacgt tttgggcacg agattagcga agatgctcta
    10741 cgcgatgcca ttgtgctgaa aaaccgcgaa cgtcgcgcac tggccaactt ttatcatttg
    10801 gggcagttaa atcctccggc gcttagcggc agcgacattc tgaaagtagt ctacggcgca
    10861 accttccggt tcgataaaga ggcgttgatc aatgaactgg atgcaatgac cgcccgcgtt
    10921 cgtcagcagt gggaagaagg ccagcgactg gacccgcgtc cgcgcatttt aatcaccggc
    10981 tgcccaattg gcggcgcagc agagaaagtg gtgcgcgcaa ttgaagagaa tggcggctgg
    11041 gttgtcggtt atgaaaactg caccggggcg aaagcgaccg agcaatgcgt ggcagaaacg
    11101 ggcgatgtct acgacgcgct ggcggataaa tatctggcga ttggctgctc ctgtgtttcg
    11161 ccgaacgatc agcgcctgca aatgctcagc cagatggttg aagaatatca ggtcgatggc
    11221 gtagttgatg tgattttgca ggcgtgccat acctacgcgg tggaatcgct ggcgattaaa
    11281 cgtcatgtgc gtcatcagca caacattcct tatatcgcta ttgaaacaga ctactccacc
    11341 tcagatatcg ggcagctcag cacccgtgtc gcggccttta ttgagatgct gtaaggagtg
    11401 gcagtggcat attcgattgg cattgattcc ggctcaaccg ccaccaaagg gatcttactg
    11461 gcagacggcg tgattacgcg ccgtttcctc gttccaacac cctttcgccc ggcaacagca
    11521 attactgaag cctgggaaac tctgcgcgaa gggttagaaa caacgccgtt tctgacgctc
    11581 accggctacg gacggcaact ggtggatttt gccgataaac aggtgacgga aatctcctgt
    11641 cacgggctgg gcgcacggtt tcttgcgcca gcaacgcgcg cagtaatcga catcggtggt
    11701 caggacagca aagtgattca gcttgatgat gacggtaacc tgtgcgattt cctgatgaat
    11761 gacaaatgcg cggcgggcac cgggcgtttc ctggaggtga tctcgcgcac gcttggcacc
    11821 agcgtcgagc aactcgacag cattaccgaa aatgtcatac cgcacgccat cacgagtatg
    11881 tgcacagtgt ttgctgaatc agaagtgatc agcctgcgct ctgcgggcgt agcgccagaa
    11941 gcgattctcg ccggagtgat taacgcgatg gcgcggcgga gcgccaattt cattgctcgt
    12001 ctctcctgtg aagcgccgat tctgtttact ggtggcgtta gccactgtca aaggtttacc
    12061 cacatgctgg aaagccatct gggaatgccg gtacaaactc accctgatgc gcaattcgct
    12121 ggtgcaattg gcgcggcggt gatcggtcag cggcagagaa aacgcgcatg aaagagtatc
    12181 tgtttttatt tcactccacg gtcggcgtca tacaaacccg caaggcgttg caggcagcgg
    12241 gcatgacttt tcgcgtcagc gatattcccc gtgatttacg cggcggctgc gggttatgca
    12301 tttggctgac ctgtccgcct ggcgaggaaa ttcaatgggt gatccctggg catacggaat
    12361 cagtttattg tcagcaggat ggtggctggc gctgcattgc acattacggg atttctccgc
    12421 gttaaggatt cattaagatc gctacgtata cttctcgata tcttattgat ttgccttaaa
    12481 gggattaccg tgacaaaaag tatttcgtta agtaaacgca tcttcgttat tgtgattctt
    12541 tttgtcattg tggcagtgtg tacattcttc gtgcaatctt gcgcccgtaa aagcaatcat
    12601 gcagcatcat ttcagaatta ccacgccacc attgacggca aagaaattgc aggcataacg
    12661 aataatatct cttcgctgac ctggtcggca caaagcaata ctctttttag cactatcaac
    12721 aaacctgccg ccattgttga aatgaccacc aatggcgatt tgatccgcac aattcctctg
    12781 gattttgtta aggatctcga gacgatcgaa tacattggcg ataatcagtt tgtcattagc
    12841 gatgagcgcg attatgccat ttatgtgatt tcactgaccc caaactcgga agtaaaaatc
    12901 ctcaaaaaaa taaaaatccc gctgcaggaa tccccaacca attgcggttt tgaaggattg
    12961 gcctattctc gccaggatca cactttctgg ttttttaaag agaaaaaccc gattgaggtc
    13021 tacaaagtta acgggctgtt aagtagcaat gaattgcaca tcagcaaaga caaggcacta
    13081 caacggcaat ttactttaga tgatgtgtcc ggcgcagaat ttaaccaaca aaaaaatacg
    13141 cttctggtgc tgtctcatga atctcgcgca ctccaggaag taacgctggc tggcgaggtt
    13201 ataggtgaaa tgtcactaac caaaggcagt cgaggtttgt cgcataacat caaacaggcc
    13261 gaaggggttg cgatggatgc ctcaggtaat atttatatcg ttagcgaacc aaatcgtttt
    13321 tatcgcttta cccctcaatc atcgcattaa caggctggca tgtttcacct ttttgttatt
    13381 aacttgttaa caacaaaaat gacaaacaag gttaaacatg ccttcgccct cgcatcccgt
    13441 agaacgcttt tctttcagca ccgcgctttt cgggatgctg gttctgacct taggtatggg
    13501 tttaggccgc ttcctttata cgcctatgtt gcccgtcatg atggcggaag gatcgttttc
    13561 atttagccag ctctcgtgga ttgccagcgg caactatgcg gggtatctgg ctggcagtct
    13621 gctattttcg tttggcgcat ttcaccagcc atcgcgcctg cgcccatttc tgttggcttc
    13681 tgccctggcg agcgggttgt tgatcctcgc aatggcatgg ttgctgccat ttattctggt
    13741 gttattgatt cgcgtcctgg cgggtgtcgc cagcgccggt atgctgattt ttggttcgat
    13801 gctgattatg cagcacacgc gccatccttt tgtgctggca gctttgtttt ctggcgttgg
    13861 cattggcatc gcactgggca atgaatatgt tctggcaggc ctgcattttg acctctcctc
    13921 gcaaacgtta tggcaaggcg ccggggcgct ttctggcatg atgctgattg cacttacgct
    13981 tttaatgccc tcgaaaaaac acgccatcac accaatgcca ttggcaaaaa cggagcaaca
    14041 gataatgagc tggtggttac tggctattct gtacggcctg gcgggatttg gttatatcat
    14101 cgtcgcgact tacctgccgc tcatggcgaa agatgcaggc tcaccattgt taaccgccca
    14161 tctctggacg ttagtcggct tatcaattgt gcctggttgc tttggctggc tatgggcagc
    14221 aaaacgttgg ggggctttgc cctgcctgac ggcgaatttg ctggtgcagg ctatctgtgt
    14281 gctgcttact ctcgccagcg actcgcctct cttgcttatc atcagcagtc ttggttttgg
    14341 cggtaccttt atgggcacga catctctggt gatgactatc gcccgccaac tgagcgtacc
    14401 gggaaatctc aatcttttag gctttgtgac gctcatttac ggcattggac aaatcctcgg
    14461 cccggcgctg accagtatgc tcggcaatgg aacatcggcg cttgccggcg ccactctctg
    14521 cggcgcggcg gcgttgttta ttgctgcgct tatcagcaca gtacaattat ttaagctgca
    14581 agtggtcact tcatgaggag tgtttccctt accactcaag aaattcgacc gcaaccgttg
    14641 atgtcagcca gaccccgttt tccgcttgcc agaaagggag gcctcgtttc gccatttcct
    14701 gtgctttgac ggttaaaata accggcgatc cgtgtcttgc tcccactttg cgtgccgttg
    14761 cttcatcagc agagagatgt acgtagtgac gctcacctgc aatcagcccc tgttttttta
    14821 tctcgtcaag gaaacggctt gccgtaccgt ggtagagaaa ctgtggcggt gttttttcgg
    14881 caaaggaaat agcgacctgc gaagtcgaat gcccctgaac cgcgcggatg cacctaccat
    14941 cactggaata actaaaacgc tttttatcgc tggtggcaac cacggtatcc agcaatgcac
    15001 gggttaaccg cttgcccact ttttgggcac aaagtatgag cttatcgata tccgcccatc
    15061 cttcacggtc cagtacgata ccaatcgcct ccggtttgtg tcggaggaca aaacttaaaa
    15121 acttgctcgt atccgccagc tctttttcgt tgtactttgc catcgcgttt tcgtccctgg
    15181 attatctgtt ttttatttac tatgtcattg tcactgggtt tatgaatttt ctgcaactca
    15241 atcctgcgta gaaccttttc cacacagcgt aattttaccg ctatgcaaaa aacgcatagt
    15301 cagaaacata cttgcatttt ccccataccc acatggctga taaggttttt cagctccagg
    15361 aattttcctg gctcaagcaa cacacaaaca caaaaaaaca atggggattg ttatgacaca
    15421 acaaggggat gctgtagccg gggagctggc gacagaaaaa gtgggcatta aagggtatct
    15481 ggcttttttt ctcaccataa tctttttttc cggtgttttt tccgggacag acagttggtg
    15541 gcgcgttttt gatttttccg ttctgaacgg atcgtttggg caactgcctg gtgcgaacgg
    15601 agccaccacc tcgtttcgcg gcgcgggtgg cgcgggggct aaagatggct ttctctttgc
    15661 gctggagctg gcaccgtcag tcattctctc tctggggatt atttcaatta ccgatggtct
    15721 tggcggatta cgcgccgcgc agcagttaat gacgccagtg ctaaaaccgt tgttgggcat
    15781 tccagggatc tgctcgctgg cgctgatcgc taacctgcaa aacaccgatg ccgccgcagg
    15841 tatgaccaaa gagctggcgc aggaaggtga aattaccgaa cgcgataaag tgattttcgc
    15901 cgcatatcaa accagcggta gcgccattat caccaactac ttctcttccg gcgtcgcggt
    15961 gtttgccttt ctgggcacgt ccgtaatcgt acctttagca gtcatcctgg tgtttaaatt
    16021 tgtcggagcc aacattttgc gcgtctggct taacttcgaa gaacgtcgca acccaacgca
    16081 aggagcgcaa gcatgacaac tcaggtccgc aaaaacgtca tggatatgtt tatcgatggt
    16141 gcgcgccgtg gctttactat cgccacgacc aacctgctgc caaacgtggt gatggcgttt
    16201 gtcatcattc aggcgctgaa aattaccggc ctgctcgact gggtggggca tatctgtgaa
    16261 cccgtaatgg cgctgtgggg attacccggt gaagcggcaa ccgtcctttt agccgcatta
    16321 atgagtatgg gtggcgcagt cggtgttgcc gccagtctgg caaccgctgg cgcattaacc
    16381 ggtcacgatg tcaccgtttt actgcccgcg atgtatttaa tgggcaaccc ggtgcaaaat
    16441 gtcggtcgct gcctggggac agccgaagtg aatgccaaat attacccgca tatcattacg
    16501 gtctgcgtga ttaacgcatt gctgtcgatc tgggttatgc agcttattgt ttaacaagga
    16561 gttaccatga ttgattatac cgcagccggt tttaccctgc tgcagggagc gcatttgtat
    16621 gcgccggaag atcggggaat ttgcgatgtc ctcgtcgcta acggcaaaat tatcgccgtt
    16681 gccagtaata tcccttctga cattgtaccg gactgcacgg ttgtcgatct cagcgggcag
    16741 atcctctgcc caggttttat tgatcaacac gtccatttga ttggcggtgg cggcgaagca
    16801 ggtcccacga cgcgcacgcc ggaagtggcg ttaagtcgtc tgacggaagc aggcgtcacg
    16861 tcagtggttg gtctgctggg caccgactct atctctcgcc acccggaatc cctgctcgcc
    16921 aagacccgtg cgctcaatga agaaggcatc agcgcctgga tgctgaccgg cgcttatcat
    16981 gtcccttccc gcactattac gggttccgtg gaaaaagacg tggcgattat cgatcgcgtg
    17041 attggcgtga aatgcgccat ctctgatcac cgttctgccg caccggacgt ttatcacctg
    17101 gctaatatgg cggcggaatc ccgcgttggc ggtttgctcg gcggtaaacc tggcgtcacc
    17161 gtgttccaca tgggcgacag caaaaaggcg ttacagcctg tctatgacct gctggaaaac
    17221 tgcgatgtgc cgattagcaa gttgctgccg acccacgtta accgcaacgt tccgttgttt
    17281 gagcaggcgc tggagttcgc gcgcaaaggc ggcaccatcg atatcaccag cagcattgac
    17341 gaaccagtcg cccctgccga aggtattgcc cgcgccgttc aggcgggtat tccgctggca
    17401 cgcgtcaccc tcagctccga cggcaacggt agccagccgt tcttcgatga cgaagggaat
    17461 ttaacccata tcggtgttgc cggttttgaa acgttgctgg aaaccgtgca ggtgctggtc
    17521 aaagattatg atttcagtat cagcgatgcc ctgcgcccgc tcaccagtag cgtagccggt
    17581 ttccttaacc tgagcgggaa aggcgaaatt ctgccaggca atgacgcaga cttactggtc
    17641 atgacgccag aattacgcat tgagcaggta tacgctcgcg gcaagctgat ggtcaaagac
    17701 ggcaaagcct gcgtgaaagg aacgtttgaa acggcataaa cggttgtcag ccgcccgaat
    17761 ggacgtagaa tgcccgcaag ggcggctgac agagtaaaac gtaatggatg actgtggtgc
    17821 ggttttgcat aatattgaaa ctaaatggct ttatgatttt ctgaccctgg aaaaatgccg
    17881 caatttttcc caggcagcag tcagtcgcaa cgtctcgcaa ccggcattca gccgccgcat
    17941 ccgtgcgctg gaacaggcga ttggcgttga attgtttaac cgccaggtga cgccgctgca
    18001 actctcggaa caaggtaaaa tattccattc gcagatccgc catctgttgc aacagttaga
    18061 gagcaacctt gcagaactcc gtggtggcag cgattacgcg caacgcaaaa ttaaaatagc
    18121 cgctgcacac tctctttccc tcgggctgtt accgtccatt atcagccaga tgccgccgct
    18181 ctttacctgg gcaattgaag ctattgatgt cgatgaagcg gtcgataaac tgcgtgaagg
    18241 gcaaagtgac tgtatttttt cctttcacga cgaagatttg ctggaagcgc cgtttgacca
    18301 cattcgctta tttgaatctc aattgttccc cgtttgcgcc agtgacgaac acggagaagc
    18361 actttttaac ctcgcgcagc cacactttcc gttactgaat tacagccgca actcctacat
    18421 ggggcgattg attaatcgca ccctgacgcg ccacagtgag ttaagtttca gcaccttttt
    18481 tgtctcttcg atgagcgagc ttttaaagca ggttgccctc gacggctgtg ggattgcctg
    18541 gctaccggaa tacgccatcc aacaagaaat tcgcagcggg cagctcgttg tactgaatcg
    18601 ggacgaactg gtgatcccga ttcaggccta tgcataccgg atgaataccc gcatgaatcc
    18661 agttgccgaa cgcttctggc gtgaactgcg cgagctggag attgtgctta gctgacattc
    18721 tccagcgtcg cactgcgcca accgccatta tcccagacca gatcaaacgt tagcgattca
    18781 tcgtcgtgat acagcattat ctcaaggctg atcgccagag tgtggcagta cgcttctttc
    18841 aaattaacct gtaacgccgc aatccgtggc tcatgaaata acagtgcaga acgcagctga
    18901 cggcaatacc agctctggag atgtttgtcg ctcgggttat gcacttgcca ctcgggcatg
    18961 cctgaaacaa gtaatgacgg taatgccgaa tgaggagaat taaaaagagt gaggagatcg
    19021 ctgcatactg atttacgcag cggtgacgtt ttattgcctg aaatttcagg taaaacagcc
    19081 tgaagtgatt ggcgcatcat tttgcgcact cctgacgttt agcaacatat gcggcttgcc
    19141 ctgaacaccg ggcaccactg tattgatgct cattttccat agccattccc ttttgttcat
    19201 tacatccatg tcagtctgag acaggcctga atcctgtcag caaagtataa aaccttactt
    19261 gcctatgaat atctactgac ttttcaggaa aaaagtcatg atttgataaa aatcatcaaa
    19321 ctcaaaaaaa aaccgctagt atattctgaa tattccg
//
LOCUS       DACADF010000083        19246 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.084, whole genome shotgun sequence.
ACCESSION   DACADF010000083 DACADF010000000
VERSION     DACADF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.084"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            206..1303
                     /gene="mltA"
                     /locus_tag="ISB30_002891"
     CDS             206..1303
                     /gene="mltA"
                     /locus_tag="ISB30_002891"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAP1990503.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1380..2186
                     /gene="tcdA"
                     /locus_tag="ISB30_002892"
     CDS             1380..2186
                     /gene="tcdA"
                     /locus_tag="ISB30_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAP1990504.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2237..2680)
                     /gene="csdE"
                     /locus_tag="ISB30_002893"
     CDS             complement(2237..2680)
                     /gene="csdE"
                     /locus_tag="ISB30_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAP1990505.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2680..3885)
                     /gene="csdA"
                     /locus_tag="ISB30_002894"
     CDS             complement(2680..3885)
                     /gene="csdA"
                     /locus_tag="ISB30_002894"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAP1990506.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEASRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4077..4304
                     /locus_tag="ISB30_002895"
     CDS             4077..4304
                     /locus_tag="ISB30_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAP1990507.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4655..5572
                     /locus_tag="ISB30_002896"
     CDS             4655..5572
                     /locus_tag="ISB30_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAP1990508.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGENPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5591..5986
                     /locus_tag="ISB30_002897"
     CDS             5591..5986
                     /locus_tag="ISB30_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAP1990509.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5979..7079
                     /gene="rlmM"
                     /locus_tag="ISB30_002898"
     CDS             5979..7079
                     /gene="rlmM"
                     /locus_tag="ISB30_002898"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAP1990510.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7123..7854)
                     /gene="fucR"
                     /locus_tag="ISB30_002899"
     CDS             complement(7123..7854)
                     /gene="fucR"
                     /locus_tag="ISB30_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAP1990511.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7912..8334)
                     /gene="fucU"
                     /locus_tag="ISB30_002900"
     CDS             complement(7912..8334)
                     /gene="fucU"
                     /locus_tag="ISB30_002900"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAP1990512.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8336..9754)
                     /gene="fucK"
                     /locus_tag="ISB30_002901"
     CDS             complement(8336..9754)
                     /gene="fucK"
                     /locus_tag="ISB30_002901"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HAP1990513.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAVVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATSIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9863..11638)
                     /gene="fucI"
                     /locus_tag="ISB30_002902"
     CDS             complement(9863..11638)
                     /gene="fucI"
                     /locus_tag="ISB30_002902"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAP1990514.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11671..12987)
                     /gene="fucP"
                     /locus_tag="ISB30_002903"
     CDS             complement(11671..12987)
                     /gene="fucP"
                     /locus_tag="ISB30_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAP1990515.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13534..14181
                     /gene="fucA"
                     /locus_tag="ISB30_002904"
     CDS             13534..14181
                     /gene="fucA"
                     /locus_tag="ISB30_002904"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAP1990516.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14209..15357
                     /gene="fucO"
                     /locus_tag="ISB30_002905"
     CDS             14209..15357
                     /gene="fucO"
                     /locus_tag="ISB30_002905"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP1990517.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTDEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15462..16307)
                     /gene="xni"
                     /locus_tag="ISB30_002906"
     CDS             complement(15462..16307)
                     /gene="xni"
                     /locus_tag="ISB30_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311685.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAP1990518.1"
                     /translation="MRGLFPISHPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKAGRPP
                     MPEELHNEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(16329..17696)
                     /gene="sdaB"
                     /locus_tag="ISB30_002907"
     CDS             complement(16329..17696)
                     /gene="sdaB"
                     /locus_tag="ISB30_002907"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAP1990519.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17754..19043)
                     /locus_tag="ISB30_002908"
     CDS             complement(17754..19043)
                     /locus_tag="ISB30_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAP1990520.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4706 a         4765 c         5095 g         4680 t
ORIGIN      
        1 gtggatgcgc actatactct gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttggcat gattttgaac aaaaacaaat cactagagcc tattttttgt gcaaaatttg
      121 cgtcagggag aggctgctca tttcattaaa cgcggtggtt tgttatcttc gttgcgcctt
      181 attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg
      241 cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg gacagcaata
      301 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      361 gccgattaat gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      421 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      481 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgtcgacaa
      541 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
      601 tcagggcgag ttccagtatc cgatttaccg tatgccgcca aaacgtggtc gtctgccgtc
      661 tcgtgcggag atctacgcag gagcgctgag tgataaatat attctcgctt acagtaactc
      721 cctgatggat aacttcatta tggatgtgca gggtagcggg tatatcgact ttggtgatgg
      781 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
      841 taaggtgttg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
      901 tcactggggc gaaacacaca gtgaagctga ggttcgcgag ctgctggaac agaacccgtc
      961 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1021 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1081 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1141 gatggtggcg ctggatgtcg gtggcgcaat caaaggccaa cacttcgata tctatcaagg
     1201 gatcgggccg gaagccggac accgcgcagg ttggtacaac cactatggac gtgtctgggt
     1261 gctgaaaacc gccccgggcg caggtaacgt ctttagcggc tgatgtgata ttctgtatgc
     1321 cgtttttacg gatatgacag ggtgaggggt aacctcaccc tttttaattc tgaggtgcta
     1381 tgtctgtggt aattagtgat gcatggcgtc agcgttttgg tggcacagcg cgtctgtatg
     1441 gtgaaaaagc attgcaactg tttgctgacg cgcatatttg tgtggttggt atcggtggtg
     1501 tcggttcctg ggcggcggaa gcgctagcgc gcacggggat tggtgcaatc acgcttatcg
     1561 atatggatga tgtgtgcgtc accaatacca atcggcaaat tcacgctctg cgcgataacg
     1621 tcgggctggc aaaagcggaa gtcatggcgg agcgtattcg ccagattaac ccggagtgcc
     1681 gtgtaacggt agtggatgac ttcgtgacgc cggataacgt agcgcaatat atgaatgccg
     1741 gttattcgta cgtgattgat gctattgata gtgtacggcc caaagcggcg ctgattgctt
     1801 attgtcggcg caataaaatc ccgctggtca cgaccggtgg cgctggtggg cagattgacc
     1861 cgacgcagat tcaggttacc gatctggcga aaacgattca ggacccgttg gcggcgaagt
     1921 tgcgcgagcg cctgaaaagc gattttggcg tagtgaaaaa cagtaaaggt aagctcggcg
     1981 tggattgcgt gttctctacc gaagcgctgg tgtacccgca gtcagacggc accgtctgtg
     2041 cgatgaaagc cacggcggaa gggccaaagc ggatggattg tgcctcagga tttggcgcgg
     2101 cgacgatggt gaccgccact tttggctttg ttgcggtttc tcatgcgctg aagaagatga
     2161 tggcgaaagc ggcgcgtcag gggtaaacaa tcaggtcgga tgcgacgcga acgtcttatc
     2221 cgacctggtt ctgtgcttaa acctgcttcg ccgcagcgat aatcgcctcg cttaacgcat
     2281 tcaacccctg gctgcgtgag gcgctaagtt gcgcacgtaa tcccagctca tcaaacaatg
     2341 ccagcggcga ctgtgcctgc aattcggcag cggttttccc ctcaacggca gtcaacaaca
     2401 ccgccaacag gccgcgcaca atgcgccctt cgctgtcgcc aaagaaatgc atcttgccgt
     2461 tttcagccac ggtatatccc agccagacgc ggttttcgca tccggcaatc tctttagcct
     2521 gcgcttttaa ctcctctggc aaagccggaa gctgtttccc cagcatgatc aactgccgat
     2581 atttatcttc ccattgcgtc aacggtgcga aggtattgcg taacgtttct gcggttacgg
     2641 ttgtgccgaa cggatgtccg gcgaattgcg ggtttgtcat taatccacca ataattccag
     2701 cgcgcggtca acggcattca ccagcgcatc cacatcactc tttgtattat atggcgcaaa
     2761 tgaggcgcgc agtgtgccgg ttacgcctaa ttctgccagt agcggctgag cgcaatgctg
     2821 cccggcgcgc agggcaatac cgtactccgc cagcagtgtc accatatcgc tgtgatgaac
     2881 tccagcaaaa tcaaaggcca gcaggctgga atcctggcag cggaatgaac gaaagcctgg
     2941 acgtttcgcc agcgcttctt ctgcaagcgt tgctaagcta cggctccagc tttcggcttg
     3001 gttgatatcg taatctgtca gccattccag cgccgcgctt aatcctatga cgccagcgac
     3061 atttggcgtt cctgcttcca gtttccacgg cgcagattga gtcgtgaaac cgtcaaaact
     3121 cacttcgtga accattttgc cgccgcccag ccagggcgac atcgcttcca gcagttctgg
     3181 tttgccatac agcacgccga tgcctgtcgg accatacagt ttgtgacctg aaaaagcata
     3241 gaaatcaata tccagttgct gaacatccgc ggggaaatgc accgccccct gagcaccatc
     3301 aaccatcacc accatcccgg ctgaatgagc aaaagtaatc gctctcgcca gatccgggca
     3361 accgccagtg acgttcgaca tctggcccaa cgccagaatc cgactacggg gagtaatcag
     3421 ttctggcaac aaatcgacat ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt
     3481 ggcccccgtt tgttgggcga ccatcagcca ggggacgagg ttggcgtggt gttctgccac
     3541 actgacaata atttcatcgc ccggttgcag acgcggacgg gcatagcatt gcgccaccat
     3601 gttgatggat tcagtggtgc cgcgcgtcca gacgatattt ttgtcatccg atgcattcag
     3661 taattgtgcc actttttccc gcgcagcttc gtaacgcgcg gtcaggcgtt gggcttcggc
     3721 aaactggctg cgatggacgt ttccggcgct caggctataa aactgtcggg aggcttcaac
     3781 cacggcttca ggtttaagcg cggtcgcggc gctgtcgaga tagacgcccg catcctgtag
     3841 cgcgggaaac tgggcgcgaa actgcgcggg attaaaaacg ttcatggtac tcctcggctt
     3901 gatgcaccga tcgtgacgca aaattcactt tgatacaagg agagtgctaa ccatcagaat
     3961 tatctggata tcctggcgaa gccgcgtcag taacatatgc tgaatagtgt taggcgttaa
     4021 ttttagcctg ttaatcaata ctattaagat ttattaatcg ctttaaggag aagatgatga
     4081 aaaagactgc cgcaattatt tctgcctgta tgctgacttt tgccctgagc gcctgttccg
     4141 gttcgaacta tgtgatgcac accaatgacg gacgtaccat cgtctctgac ggcaaaccac
     4201 agactgataa cgataccggt atgatttcgt ataaagacgc taatggcaac aaacagcaga
     4261 tcaaccgtac cgacgtgaaa gagatggtcg aactggatca gtaattcgcg atcgcaaggt
     4321 aaaaaaaagc accgcaatta ggcggtgcta cattaatcac tatggacaga cagggtaaat
     4381 gtacaggaag tgaaaaaagg tagctttgct accatggtct gaatcgcaga ccaattgcaa
     4441 acacaacaac acaacatcac aaccgtaagc caaaagttca ccagaacacg cattccgata
     4501 aaacttttcg ttccggctca ggaagtgccg ccactatagg tatttgctgg tagaagctca
     4561 acggacaatt tataatggct cagattaaaa aaactaatag gttacacagt gtaatctaat
     4621 tgttaaattc atttaacatc aaagtttaaa agccatgtct aaacgattac caccgctaaa
     4681 tgccttacga gtttttgatg ccgcagcacg ccatttaagt ttcactcgcg cagcagaaga
     4741 gctttttgtg acccaggccg cagtaagtca tcaaatcaag tctcttgagg attttctggg
     4801 gctaaaactg ttccgccgcc gtaatcgttc actcctgctg accgaggaag ggcaaagcta
     4861 tttcctcgat atcaaagaga tattttcgca attaaccgaa gcgacgcgta aactccaggc
     4921 ccgtagcgct aagggggcgt tgacggtcag tttactcccc agtttcgcca ttcattggtt
     4981 ggttccgcga ctttccagct ttaattcagc ttatccggga attgatgttc gaatccaggc
     5041 ggttgatcgt caggaagata agctggcgga tgatgttgat gtggcgatat tttatggtcg
     5101 gggcaactgg ccggggctac gggtggaaaa actgtacgcc gaatatttat tgccggtgtg
     5161 ctcgccgcta ctgctgactg gcgaaaaccc cttgaagacc ccggaagatc tggctaaaca
     5221 tacgttatta catgatgcgt cacgccgtga ctggcagaca tatacccgac agttggggtt
     5281 aaatcatatc aacgttcagc aagggccaat ttttagccat agcgccatgg tgctgcaagc
     5341 ggctatccac gggcagggag tggcgctggc aaataacgtg atggcgcaat ctgaaattga
     5401 agccggacgt cttgtttgcc cgtttaatga tgttctggtc agtaaaaacg ctttttatct
     5461 ggtttgtcat gacagccagg cagaactggg taaaatagcc gcctttcgcc aatggatcct
     5521 ggcgaaagcc gctgctgaac aagaaaaatt ccgctttcgt tatgaacaat aatttacgta
     5581 gggtacgacc atgaccagcc gttttatgct gattttcgcc gccattagcg gcttcatttt
     5641 tgtggctctg ggcgcttttg gcgcgcatgt gttaagtaaa accatggggg ccgttgagat
     5701 gggctggatc cagaccggcc tcgaatacca ggcgtttcat acgctggcga tcttaggtct
     5761 ggcggtggca atgcagcgtc gcatcagtat ctggttttac tggagtagcg ttttcctcgc
     5821 gttaggcacg gtgctgttca gcggcagcct ttattgcctg gcgctgtccc atctgcgttt
     5881 gtgggcgttt gttacaccgg ttggcggcgt gagcttcctt gcgggttggg cgttaatgtt
     5941 agttggtgct atccgtttaa agcgcaaggg cgtaagtcat gaataaggtt gtattgctgt
     6001 gccgtccggg ctttgaaaaa gagtgcgccg cagaaattac cgataaagcc ggccagcggg
     6061 aaattttcgg ttttgcccgc gtgaaagaga atgcgggtta tgtgatttat gaatgttatc
     6121 aacctgatga tggcgataag ttaatccgtg agctgccgtt cagttcatta atttttgccc
     6181 gccagtggtt tgtagtgggg gaactcctgc agcatttgcc gccagaagat cgtattaccc
     6241 ccattgttgg catgttgcag ggtgtagtag agaagggcgg tgaactgcgt gttgaagttg
     6301 ccgataccaa cgaaagcaaa gagttgctga aattctgccg taaattcacc gtgccgctac
     6361 gcgctgcctt gcgtgatgcg ggggtgctgg cgaactatga aacgccgaag cgtccggttg
     6421 tgcatgtatt ctttattgca ccaggctgtt gctataccgg ttactcatac agcaacaata
     6481 attcgccgtt ctatatgggc attccgcgcc tgaaatttcc ggcagatgcg ccgagtcgtt
     6541 ccacgctcaa actggaagag gcatttcatg tgtttattcc cgcggatgag tgggatgaac
     6601 gcctggcgaa cgggatgtgg gcggtggatt tgggcgcttg cccaggcggc tggacctacc
     6661 aactggtgaa gcgcaacatg tgggtttatt ccgtcgacaa cggcccgatg gcgcaaagtc
     6721 tgatggatac cgggcaggtg acgtggctgc gggaagacgg tttcaaattc cgtccgacac
     6781 gcagcaatat ctcctggatg gtatgcgata tggttgaaaa accggcgaaa gttgcggcat
     6841 taatggcgca gtggctggtt aatggctggt gccgtgaaac tatcttcaat ctcaaactgc
     6901 cgatgaaaaa acgctacgaa gaagtgtcac acaatctggc gtatattcag gcacagcttg
     6961 atgaacatgg cataaatgct cagattcagg cgcggcagtt gtatcacgat cgcgaagaag
     7021 tgacggtgca cgtccgccgt atctgggctg cggtgggtgg tcgtcgcgac gagcgataac
     7081 aggcaaggcc ggggcgtcgt ctccggcctg ctaccctttc cgtcaggctg ttaccaaaga
     7141 agttgcaacc tggcgctcat cagaaataat gtgcgtgacc tcatccagat gcccgatgcg
     7201 ggcttcccct gaacgattaa atttactttt atcaatcaat aacaacgatt gcgcggcacg
     7261 ttttaataac attgatttgt aatcagcgtt gatggcattg gagtcccaca gtgcgccgct
     7321 gctatcgatc ccttcacaag aaaaaataaa cagatcgatt tccagcgatt taagttggga
     7381 aatcagcgag ggattgacgt aacagccata tttgcgctca agtgtgccgc cggaactgat
     7441 cagttgaatg cgttcgcgtt taccgagttc atggcaaatc ggatggctat tggtgaagac
     7501 ctggatgttg atgtcaggca actggcgtgc cagataccag caggttgaac tggcgtctaa
     7561 ggctatcacc atcccttctt caatccacgc gagcgcctcg cgcgcgatat ctgctttatg
     7621 cgcataatgg cttttcagcc ggatgtgaaa gggatcgcca ctgtcttgat tttgacggtg
     7681 gatatattta gcgcgcccat gattgcgcag aattttaccc tgcgtctgta attcattgag
     7741 atcgcgacga atggtttctt tactgacctt tagctgttca gagagagctt ccgtggtcag
     7801 gctggtatgg ttcagcagca ggtcgactat cgcttgctgg cgtgccgctt tcatattttt
     7861 caccccatca ttcgataacc gcccgcgtca tgcgggcgta ccggtatgag attacggtgt
     7921 tacccctttt tttaaaagaa tattcccgta cttcgctcgt tcgcctgtga taacgatcgc
     7981 aaaggctttt tgcgcccgtt cataaaacgc aaaacgattg atgcggatga tgtcaggaca
     8041 cggggcttgt agtgaaagcg cattacggta acgtcgttct acttcaggat cgagagtgtc
     8101 accttctacc gccgccatca tcaccagcgg tggtgcataa ctgtccagtt caaataacgg
     8161 gataatcgcc tggagcaagt cgctcaccaa caggccatca gcgcggatca cctgcggtcc
     8221 catcgaatgg gcgggaaagt gagcatcgga aaaaataatt tcatctccat gccccatctc
     8281 tgccagcact ttcaatagtt cgggagaaat taacggcgaa attgttttca gcatttcaca
     8341 cctcctctat aaattcaggt tcagtttgcg ggtagaaata acggtactga taatgaatct
     8401 gcgcgcgggc ttcttccggg ctgttaaatt cccctacgcc ataccaacca aacagcgcag
     8461 ctcctgcgac ggtcgtttcg gcatcgtcga gaacttttac cggaatatca agcatattgg
     8521 ctttaatctg attccacaat gtgttgcgac ttcctccgcc gactaacaat aattcagagg
     8581 ctttaaagtg cccgattttt tccagcatct gtagattgcg ctgtaattgc gtggttaacc
     8641 cttccagcgc cgcgcgatag aaatgccccc gcgtggtatt aagcgttacg ccttgccagc
     8701 cagcgttctg gcacgacaat aaatcacact gcattttcac tccatctgcg ccaggcgcga
     8761 tcaggcgagc ttcttcaatc aacatttgcc agggagtttc agccgtccag aacagttttc
     8821 tcacccattc cagcacgccg gatgccagcc attgcatacc tgggttatac aaccctgcct
     8881 ggctatccag ttcgcaggta gaaccggcgt actgacttaa cagcgaagta tcaacctggg
     8941 cgctgcggac cattaaaatt tcccatgtac cggaagagag cacgggttca ttttgttcgg
     9001 caccagcgcc aaaaagggcg aactgggtat cgtgacctgc ggaaatcacc ggtatgccaa
     9061 cgggtaagcc gagcattgct gcggcgctgt tctgtagcgt accaatctgt tcacccgctt
     9121 ccaccagacg agggaagagt cggcgtggaa tactggtggc ttgtaaaatt tgcggactga
     9181 aatcgcgttg ctggatatcc agcatctggc tggttccggc catcgtaata tcagtagtga
     9241 attcgccggt taaacggtgg ttaatcagcg acgaaataaa gagccaggcg tgcgcgcgtt
     9301 ccagcagttg tggatgattt tctttcaacc acaccaactt atataacgta ttgaaactaa
     9361 aggctccgac tccagaaata gcctgcaact gctgtgcgga gatcaaccgt tcaatattgt
     9421 ccattaccac ggctgttcgc ggacatttcc agctaatgat cggatagagc agattgcctt
     9481 gcttatctac cagagcgcca tccacaccaa aggtggtgac ggcgatacca cggatgtggc
     9541 aatcagtcag ttcactattg atttgccgac agcaatcagc aaagcgttgc aaaatggcgt
     9601 ctaaagacca ctggtgccag gtgttgtttt ccatcgcgat atcgctggca ttaggcgttg
     9661 aggcgcgggc aacaattttt ccctgccgat taaccgcgat ggccctgaca ttggtcgcgc
     9721 cacagtcgag taccaggata acttcttgtt tcataatcgc tcctgcaata tagccggata
     9781 acattgctta tccggctaac cactcttgct tgttcaggcc cggtagccgg agcgaccggg
     9841 catcacatca gggagtcatg tattaacgct tgtacaacgg gccgtagttc tggcaagcgc
     9901 ggtaatcctg gccttcaata tccatgccgt gcgcggccca ggcagaagga cgatacactt
     9961 tggtctcttc aacgttgtgc atacataccg ggatacgcag catggaggca agagtgataa
    10021 agtctgcgcc aacgtggccg atggtcagaa ctccgtggtt agcgccccag ttcgccatta
    10081 ccgagtacac atccgtaaac ggccctttac cggtgaggcg cggtgcaaac caggtggttg
    10141 gccaggttga gttggtgcgt ttgttgagga tgtcatgcac atccttcggc aattccacgc
    10201 tccagccttc cgcgatttgc agtaccggtc ccaggccttt gatgatgttg acacgagtca
    10261 tggtgaacgg gacgccgcct tcggtaagga agcgggaaga gtaaccgccg ccacggaagt
    10321 attcgtggat cgccgggcac cattcggtag cggcgaggca agcgtcagcc tcttgctgag
    10381 agatttccca gtgcggcttc atcgtcggtt taccttcgct gtcgcgttgt ttacaggagc
    10441 cgtccagcgc agcagaaccg gagttgatca aatggatgat gccgtgttct gccagcccgt
    10501 ccagtttatg ccccgttaca cgctcaattg cttctggtga ccagtaggta cggacatcgg
    10561 caaatacctg agcggtgccg gtgagctggt ggcccattaa cattgccacg ccgttaagac
    10621 tgtcgttttc ggtcgccacg acaaacggtt tgcgcacgcc attccagtca aatgaactgt
    10681 tgaggatcgc ttcagcggta tcgccattgg gatattgatc ggtccagtga cgttgcccct
    10741 ggaagcctgc agcgatggcg ttgtagccaa gtgattcttc cacgcgacca atatcggcca
    10801 gtttgctatt gccttgcatc atgtcgcgga tacacatcgc catcagtaaa ctttcgcgca
    10861 gaactgcgcg gctttgctcg gcattgcgtt gatactgttt gttattttca tcttcgccat
    10921 agcggaagtt tttatcagcc caggccagcg ccatttccag ttcggcttcg tcgtaaatct
    10981 tctgatcgat acggcgacgc agttcggtca tatccaccgc ctggactttc attcccagcc
    11041 aggattcaaa gaagttgtga tcaacgatgg aaccggcgat acccatcgaa acgccgccca
    11101 gcgacagata acttttacct ttcatgctgg cgactgctaa accggcgcgg gcaaagcgca
    11161 gcagtttttc ttcaacatcg gcaggaatcg atgtgtcatc ggcatcctga acgtcatgac
    11221 cgtaaatgga gaatgccggg atgcctttct ggctgtgagc tgccagagcc gctgccaggt
    11281 aaacagcgcc gggacgttca gtgccgttaa agccccaaat ggctttcggg cgggttggat
    11341 ccatgtcgat ggtttcactg ccatagcacc agcaaggcgt taccgtaatg gtgaggccta
    11401 cattctgact gctgaatttt tcttcgcaag cagcggcttc agccataccc gcgatacagg
    11461 tatcggaaat gacacactcg acggcagctc cgcaggcatg gcgcagtttc tcggtcagca
    11521 gtgcagccgt ggctttcgcc atattcattg tttgttcttc aagcgactca cgaacaccca
    11581 tgcgacgacc gtcaataacc gggcggatac caattttcgg taagctgatt tttttcatta
    11641 ctgattcctc actttattcg gaaaatatgt tcagttagtt gccgtttgag aacggaaacg
    11701 ggcaaagata aagatgactg cgaagcagag tgccgggatc agttcggcag tggggatgtt
    11761 gcccgctgcg tcactgacaa accccatgac cggagtgaca ataccgccgc caataatggt
    11821 cataacgatg aaggacgaac cgtatttggt gtcctggccg agattcttaa tgcccagcga
    11881 gaagattgtt gggtactgaa tcgacataaa ggcactgcat aaagtcaggg ctattaagcc
    11941 cacatgaccg ccagcgaagg ctgagatcag gcacagtgcc atagcgatta atgcgtaggc
    12001 tgccaggact ttgtgtggcg cgaagcgact gatgagccag gtaccggtga aacgaccaat
    12061 aaagaagcac accatggttc cggttaaata gttagcggca aagcctgctg tcatacctgg
    12121 aatttcttct acagcgtagc gaattaaata gctccagcag gccgtttgtg cgccaacgta
    12181 gcagaattgc gctaatactg cccagcgcca gtggcgaata cgcgccaggc gagaaagcga
    12241 tgcggagaac gatccttgtt tggcgtcact gtgattatca ctctgcaatg ccgggaattt
    12301 cgtcagcatg atcagcaggg cgaccagtaa cacgatagcc acgatgatca tataaggtgt
    12361 ctgtaccgat aataccaagc tgtgtttata cgcactcaat tgctctggag acattttatc
    12421 gagaacgtct tgcgattgat gtggcacgtt agacaaaata agactttgcc caaagacaac
    12481 cgcgataatt gcgccaaatg agttaaatgt ttgcgcaaga tttaagcgga agtgaccact
    12541 gctttccggc cctaataccg taacaaaagg gtttgcggca gtttccagac aacctaatcc
    12601 ggctgcaata ataaataggc caactaaaaa caaggtgtag ttcattattt ctgcggcggg
    12661 ccagaataat gcagcgccga aggcatataa aaacaatccg gtaataatcc ctgctttata
    12721 actgagtttt ttcatcaata tcccggcagg gattgggata atgaaataac caaagtaaaa
    12781 ggccgattgg atcaggccag cctggaaatt tgtcagcgta aaagcctgct ggaattgagg
    12841 caataaaatg tcgttaaggt tattggctac cgcccaaaga aaaaacagtg agcacagcag
    12901 cgcgaatgga ataatgtaac ttctgctttg ccctgcatct ttatctaccg cacggtaact
    12961 ctgcgtttgt attgatgtgt ttcccatagc atcctcttag gttcagaagc ttaagcacgt
    13021 aatcggttat gttttcaaaa gtgcaaaacc agcgagttcg ggcttcatat cagtgaatat
    13081 ttgcctgtct gaaatgaact catggattca ttttcaaata aaaaacgctc tgtaatatga
    13141 cggcggtcac acttattagc cggagggtga ctttatgtga ctaccatcac tttaataatc
    13201 gaaatttcat gcccgtttca gaaaataaat cataaaaaac acaaaccaga tgaccgtatt
    13261 aattgtttgt gccgaaaacg ggcattcctg gcagggagga gtagaaaaat gaccgttttg
    13321 ataaatattt ttaagagcgg tcatttttaa tggggttata aaaagagaac ttgcagttat
    13381 ttactgtgat tagttgaacc aggtcacaaa atataagctt gtgaatttct ttctggttaa
    13441 tcaggaagaa tatggtgagt aattaaacgg ctaattcaat agtgtgaaag gaacaacatt
    13501 attgccctgt tttgaatcag agagaggtag ctaatggaac gaaataaact tgctcgtcag
    13561 attattgaca cttgcctgga aatgactcgc ctggggctga accagggaac agcgggtaac
    13621 gtcagtgtac gttatcagga tgggatgctg attacgccta caggtattcc atatgaaaaa
    13681 ctgacggagt cgcatattgt ctttattgat ggcaacggca aacatgagga aggaaaactc
    13741 ccctcaagcg agtggcgttt ccatatggcg gcctatcaaa gcagaccgga tgccaacgcg
    13801 gttgttcaca atcatgccgt tcattgtacg gcagtttcca ttcttaaccg accgatcccc
    13861 gcgattcact acatgattgc ggcggctggc ggtaattcta ttccttgcgc gccttatgcg
    13921 acctttggaa cacgcgaact ttctgaacat gttgcgctgg ctctcaaaaa tcgtaaggca
    13981 actttgttac aacatcatgg gcttatcgct tgtgaggcga atctggagaa agcgttatgg
    14041 ctggcgcatg aagttgaagt gctggcacaa ctttacctga cgaccctggc gattacggac
    14101 ccggtgccag tgctgagcga tgaagagatt gccgtagtgc tggagaaatt caaaacctac
    14161 gggttacgga ttgaagagta atttcgtaaa gcaacaagga gaaggatgat ggctaacaga
    14221 atgattctga acgaaacggc atggtttggt cgaggtgctg ttggagcttt aaccgatgaa
    14281 gtgaaacgcc gtggttatca gaaggcgctg atcgtcaccg ataaaacgct ggtgcaatgc
    14341 ggcgtggtgg cgaaagtgac cgacaagatg gatgccgcag gactggcatg ggcgatttac
    14401 gacggcgtag tacctaaccc aacaattact gtcgtcaaag aagggctcga tgtattccag
    14461 aatagcggcg cggattacct gatcgctatt ggtggtggtt ctccacagga tacgtgtaaa
    14521 gcgattggca ttatcagcaa caacccggag tttgccgatg tgcgtagcct ggaagggctt
    14581 tccccgacca ataaacccag tgtaccgatt ctggcaatcc ccaccacagc aggcactgcg
    14641 gcagaagtga ccattaacta cgtgatcact gacgaagaga aacggcgcaa gtttgtttgc
    14701 gttgatccgc atgatatccc gcaggtggcg tttattgacg ctgacatgat ggatggtatg
    14761 cctccagcgc tgaaagccgc gacgggtgtc gatgcgctca ctcatgctat tgaggggtat
    14821 attacccgtg gcgcgtgggc gctaactgat gcgctgcaca ttaaagcgat tgaaatcatt
    14881 gctggggcgc tgcgaggatc ggttgctggt gataaggatg ccggagaaga aatggcgctc
    14941 gggcagtatg ttgcgggtat gggattctcg aatgttgggt tagggttggt gcatggtatg
    15001 gcgcatccac tgggcgcgtt ttacaacacg ccacacggtg ttgcgaacgc catcctgtta
    15061 ccgcatgtca tgcgctataa cgctgacttt accgatgaga agtaccgcga tatcgcgcgc
    15121 gttatgggcg tgaaagtgga aggtatgagc ctggaagagg cgcgtaatgc cgccgttgaa
    15181 gcggtgtttg ctctcaaccg tgatgtcggt attccgccac atttacgtga tgttggtgta
    15241 cgcaaggaag acattccggc actggcgcag gcggcactgg atgatgtttg taccggtggc
    15301 aacccgcgtg aagcaacgct tgaggatatt gtagagcttt accataccgc ctggtaaatg
    15361 tgctgatgtg ataatgccgg atgcgacgct agccgcgtct tatccggcat ataagtgcat
    15421 taaattcaat gtgttgataa tcgtttgcat ccggcacgcc gttaccttac caaccgcaat
    15481 tgctgtaaat tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga
    15541 aacgccatct ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga
    15601 ttctcatata tcccttccag actctgaaac tcgaccagta gctgcgtggc gctttttggt
    15661 ccgatccccg caacacccgg tacctttgaa ctgctgatcc ccgcaagccc ccagtaatcg
    15721 ggcaactgct gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt
    15781 ttctggaagt aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct
    15841 gtcgaaacaa tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag cgtggcggct
    15901 aagtcatcgg cttcgttgcc gctggcggac cagcacggga cgccgcgttg ctcaaaggcg
    15961 gcgcgtaatg caggcatctc gttgtgcaac tcttccggca ttggcggtcg acccgctttg
    16021 taatctggta aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg
    16081 accgcgtggg ttggctggct gtgcataatg agctgatcga gcgcgtgctg gcaggtttcg
    16141 acacagggcg aaccctgaac ggcatgaatg cgacgaataa gattcagtgc atcgacaata
    16201 agcaaatgaa cagccacgat aaccccctta aagatcaggc gataagggta acattcgatg
    16261 ccagaacagg ctatggctgg atgagaaatc gggaagaggc ctcgcaaaaa gaggcctctg
    16321 gagagcgatt aatcgcaggc aacgatcttc attgccaggc cgccgcgaga ggtttcgcgg
    16381 tacttggcgt tcatatcttt acctgtttcg tacatggtct cgataacttt atcgaggcag
    16441 acgcgcggct cgctggtacg gcgcagcgcc atacgcgcgg cgttcaccgc ttttactgcc
    16501 gcaatggcgt tacgctcgat gcacggtacc tgcacctgtc cagcaaccgg gtcacacgtc
    16561 agaccgaggt tatgctccat ggcgatttcc gccgcgatgc acacctgcgc cgggcttgcg
    16621 cctaacagtt ctgccagacc agctgccgcc atagagcacg ccacgccgac ttcaccctgg
    16681 cagccgactt cggcaccaga aatcgaggcg ttcatcttat aaagagaacc aatggcgctg
    16741 gctaccagca ggtaacgcgc cagtgagtta gcgttcactt cgcggataaa cttgtcgtag
    16801 tacgccagca cggccgggat aatcccgcac gcaccgttag tcggcgcagt caccacgcga
    16861 ccgccagcag cgttctcttc gttcactgcc agtgcaaaca tgttgatcca gtcaactacc
    16921 gccatcgggt cagtggtggt tttatcctgg ctgaccagca tccggcgtag tgccgcagca
    16981 cggcgtggaa cgcgcagttt gccaggtaac acgccttcgg tagaaatacc gcgttcaata
    17041 ccgccacgca tgacttccca gacgttcgcc aggtgctgtt ccagctcttc tttgctgtgc
    17101 agcgccagct cgtttttcat catcaggcca gagagtgaca gcccggtttc ctgacaatgt
    17161 ttttgtagat cggcagcgga actgtacgga taaggaactt caaccggtgc gctatcctgc
    17221 tgaccaaaat gctcttcatc aacgataaag ccaccgccaa tagagtaata agtctggctg
    17281 taaacgactt tatcgcccgc cagcgcggta atgcgcatac cgttttcatg cagagaaagg
    17341 ttgtcggcat ggaagttcat gcactgatca accgggaact ccacttcatg ctgaccgttt
    17401 gccagcatca ggcgaccatg agtattcaca tcctgaataa aaccagggat ggaatcgata
    17461 tccacggtat ccggcaggtt acccgccaga cccataatta tggcgatatc tgtgtggtgg
    17521 cctttaccgg tcagagagag cgagccgtac acgtcaacca ccacgcgggt cacgtcttta
    17581 agcaggttac gggcaatcag atcgtcggta aattgtttac ccgctttcat tggtccaacg
    17641 gtatgagaac tggaagggcc aatgccgatt ttgaaaatat cgaatacgct aatcatagga
    17701 aatacatcgc gttaaaacgg aggaagcgcc gcccgaaagc ggcgcgaaag gacttagctg
    17761 aacagagagt agaagattgc ggagattgca atcagaccca tcacgacaac gaatacgttg
    17821 ctgatgtgac cgctgtactt acgcattgcc ggtactttct gaattgcgta catcggcatc
    17881 aggaacagga tcatcgcgat gattgggccg cccagggttt caatcatacc caggatgctc
    17941 gggttcaggg tggcaacaat ccaggtcgtt accagcatga acagcgcagt gatacggttc
    18001 agcttgttga tttcgataga cttaccttta ccacgcagag atttaatcac cataccgttg
    18061 aagccttcac gtgcgcccag gtagtggccg aggaaggatt tggtgatagc gataatcgcg
    18121 ataatcggag ccatccacgc gatgatcggc gcgttaaagt ggttagccag gtaagacaga
    18181 atcgagatgt tctgctcttt agccgcagcc aggtctgccg gagtcaggct cagtacgcag
    18241 ctgaagacga agaacattac ggtcagcacc atcatgatgt gtgcgaatgc caggatctta
    18301 gagcatttct gttctgccat atcgccgtac tcttcacgct tcgcaacggc gaaagaagag
    18361 atgatcggag agtggttgaa cgagaacacc attaccggaa ttgccagcca cagggtcatc
    18421 cacagaccgt ttccggttgc agatgcagtg tccagagaca gcgtttccag tgctgcgccg
    18481 ttccactgcg ggatcaggta cagagccagc agcatcagta cgccaacaaa cgggaatacc
    18541 agaatactca tcgctttaac gatcatctgc tcaccgaagc gaacgatggt catcataccc
    18601 acgatcagga tcagcgacag aatcgcacgt ggcggtggcg tcatacccag ttggtgagac
    18661 atgaagcttt caactgtatt ggtgattgcc acgctataaa ccagcaggat cgggtagata
    18721 gcgaagaagt agagcagagt aatcagttta cctgcgccaa taccaaagtg ttcttctaca
    18781 acctcggtga tgtcttcgcc cgggttttta ccagacagta cgaagcgagt caggccgcgg
    18841 tgagcaaaaa acgtcatcgg gaatgcaagg atagccatga tgatcagcgg gatcataccg
    18901 ccaacaccgg cgttgattgg caggaacagc acgcccgcgc cgattgccgt gccgtaaagg
    18961 cccagcatcc acatggtgtc tgtcttgcgc caggcactac gagagtcttt cgacgcaatc
    19021 gtgctggttt gagtcgtttc catctatttc tcctggagga atgcaaataa ttcagctttt
    19081 agccagttca gcccgggaag gtgcctgacc gtaaaaatga aatctgtcta tcggcgatgg
    19141 cttaatcatt ttccaccgta ataatttgag ggcggaaaga tacacacaag ttatgtatct
    19201 atcagtgatc ttgatcccaa atacaataat ctacttggaa gataga
//
LOCUS       DACADF010000084        19205 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.085, whole genome shotgun sequence.
ACCESSION   DACADF010000084 DACADF010000000
VERSION     DACADF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.085"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            162..554
                     /locus_tag="ISB30_002909"
                     /pseudo
     CDS             162..554
                     /locus_tag="ISB30_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            607..1002
                     /locus_tag="ISB30_002910"
     CDS             607..1002
                     /locus_tag="ISB30_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990521.1"
                     /translation="MENMMKTMREHFPTRTVVIEQSLDMFGVKRNEDGTLLFPRHSDK
                     RFPIARIRKRLAAYGWRGERLNKEVARIVAGPDIYAERNEYNIIFPYGWEKKQLTREM
                     RRMFPRKTGRRVLIMLDELHHFSDLREKP"
     gene            999..1610
                     /locus_tag="ISB30_002911"
     CDS             999..1610
                     /locus_tag="ISB30_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008455304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2913 family protein"
                     /protein_id="HAP1990522.1"
                     /translation="MTLTEKSGHLAWCALVALALARQEGGVLSPAQENLFLIRWLATA
                     LKQRRFPREINPDIEWLLKQRRQLGVSAKLASKLNYLWRSCTGELSEQNDLFRLTYAL
                     ETAKDMHWNYRLLSDREWSGRNAVALSAGVNGIYLSRANLDVAFDDSGRQINPLTARL
                     TGNVVGVMKVFNRCGWQAEPESGASLPHQYSLMAGQGVPGKGD"
     gene            complement(1607..2557)
                     /locus_tag="ISB30_002912"
     CDS             complement(1607..2557)
                     /locus_tag="ISB30_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011147726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAP1990523.1"
                     /translation="MKKKPGTPTPHDATFRQFLANPDVARDFMMLHLPAELRALCDLT
                     TLKLESGTFVEDDLRQYVSDILWSMKTTTGDDGYIHLLLEHQSSADKNMAFRQLRYAV
                     AAMQRHLEAGHKKLPLVIPVLFYHGSRSPYPFSTNWLDCFSNPEQAGKVYTTGFPLVD
                     VTVIDDDEIMNHRRMAALTLLMKHIRQRDMMELLDRLPRVMVEWISPEQVRVLINYMV
                     NAGEAPAPEFMRALAERLPQHEDELMTIAQQLEQIGIEKGLQQGLQQGLQLGEQRGIE
                     KGEREATLKIARTMLQNGIDRNTVMKMTGLTEDELAQIRH"
     gene            complement(2704..2904)
                     /locus_tag="ISB30_002913"
     CDS             complement(2704..2904)
                     /locus_tag="ISB30_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990524.1"
                     /translation="MAERISQREQELLEEFLCTVLDDFSKGSITHHQAVSGIATLYTA
                     ATEGRREEALEYLREGRKLLRQ"
     gene            complement(2958..3590)
                     /locus_tag="ISB30_002914"
     CDS             complement(2958..3590)
                     /locus_tag="ISB30_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP1990525.1"
                     /translation="MFVFGYLRASTSEQNASRAKNALKAFANQNGHRIAGWYIDNVSG
                     TTMNRPELIRLLGDAEPGDVILIEQVDRLTRLDDAGWETLRKQITDKQLAIVSLDLPT
                     SHLALSSTVSDDFTRSMLKAVNGMMLDMLAAIARKDYEDRRRRQAEGICKAKTEGKYR
                     GRVADAQKHELIRTLRLAHGKSLRETARLAGVSKMTVIRVCNGNHKQDTD"
     gene            3953..5158
                     /locus_tag="ISB30_002915"
     CDS             3953..5158
                     /locus_tag="ISB30_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP1990526.1"
                     /translation="MKRDYGSVGTIALRASALLQAMSRDIEEQRKEFNLTDYHQTYTR
                     NAVAKLPKLSRRIVELAMKEMEEDGYIFNKKQIGNVEQYALTIKNVIDIYAHRQIPKY
                     RDIHKEPYVIFVVNLKGGVSKTVSTVTLAHALRVHQDLLRHDLRILVIDLDPQASSTM
                     FLDHTHSIGTVLETAAQAMLNDLDVETLREAVIRPTIIPGVDVIPASIDDGFVASQWE
                     SLVAEHLPGLKPSEVLRKTIIDRIAGDYDFVFIDTGPHLDPFLLNGLAASDLLLTPTP
                     PAQVDFHSTLKYLTRLPEMLERLEDEGVEPRLSASIGFMSKMTNKRDHETSHSLAREV
                     YASNILDSSLPRLDGFERCGESFDTIISANPVSYPGSAEALKKARTEAERFTKAVFDR
                     IEYIRGESK"
     gene            5155..6126
                     /locus_tag="ISB30_002916"
     CDS             5155..6126
                     /locus_tag="ISB30_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP1990527.1"
                     /translation="MKKVLARGRVLGNSNSEFARMLEGDGDVKTFTLKSGKQAKFVKT
                     VVLSGEIESKTFVDASVNGRDQAMLTRESVSDISRTIKLQQFFPAIGREVNGRIEILD
                     GTRRRAACIFNNVKFEILVTKDDISLADARQLAQDIQTAREHSLRELGKRLEVTYGTS
                     MTKEEIALKENLSPAKVTRAFQAAAVPDEMVAVFPVINDISLSDYQFLLKLAEEANNK
                     QTSVTELMEKVQHRLKTMPDYPAIDKSKILAAIRVESKSLTTRPTRTVQTEKLREFSD
                     RNQFARKKTDSKKRLVVYEFSRISAEAQSEIDDAIKRILKRLPESSE"
     gene            complement(6262..7533)
                     /locus_tag="ISB30_002917"
     CDS             complement(6262..7533)
                     /locus_tag="ISB30_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP1990528.1"
                     /translation="MFALADVNSFYASCEKVFRPDLRNRPVVVLSNNDGCVIARSAEA
                     KRLGIKMGVPWFQLKTAQFPEPVITFSCNYELYASMSNRVMSHLEELAPRVEQYSIDE
                     MFLDIRGIDSCINYEDFGRQLREHVRSGTGLTIGVGMGPTKTLAKSAQWASKEWRQFG
                     GVLALTSGNPKRTDKLLSRQPVEEIWGVGRRISKTLNTMGITTALQLARANPVFIRKN
                     FSVVLERTVRELNGESCISLEEAPPPKQQIVCSRSFGERVTTYEAMRQAVCQYAERAA
                     EKLRGERQFCRHIAVFVKTSPFAVNEPYYGNVASEKLMTPTQDTRDIIAAAVKALDRI
                     WVNGHRYAKAGCMLNDFTPTGVSQLNLFDDTQPRSNSNQLMKVMDGINHSGLGKVWFA
                     GRGIAPEWQMKREMLSPAYTTRWSELPVAKL"
     gene            complement(7533..7955)
                     /gene="umuD"
                     /locus_tag="ISB30_002918"
     CDS             complement(7533..7955)
                     /gene="umuD"
                     /locus_tag="ISB30_002918"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAP1990529.1"
                     /translation="MILFVTPASEPALSTAPLFTERCPAGFPSPAADYTEDELDLNAY
                     CIRRPSATYFVRAIGDSMKDMGLHSGDLMVVDKAEKPMQGDIVIAETDGEFTVKRLQL
                     KPRIALLPMNPAYPTLYPEELQIFGVVMAFIHKTRGTN"
     gene            complement(8135..8806)
                     /locus_tag="ISB30_002919"
     CDS             complement(8135..8806)
                     /locus_tag="ISB30_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015493079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HAP1990530.1"
                     /translation="MCGRFTQYNSREEYLAFLGEEAERDIPYDPEPVGRYNVAPGTRV
                     LLLSERRSQLHLDPVHWGYAPEWWNKPALINARSETAAGSRMFRPLWEHGRAIVFADG
                     WFEWKQQNGVKQPFFIYRKDGNPLLLAAIGKPPFENGNDQEGFLIVTAAADEELLDIH
                     DRRPLVYSPDAARKWLSENTTGKEAEDIAREGSLPAEDFTWHPVSRAVGNPGNQGREL
                     IEPLT"
     gene            9165..9842
                     /locus_tag="ISB30_002920"
     CDS             9165..9842
                     /locus_tag="ISB30_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAP1990531.1"
                     /translation="MSRFIQGNCVHIMSGFPDNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACHEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPPLPQNPLNDVIAWKYSGNRHHPTEKPVTSLQPLIESF
                     THPGAIVLDPFAGSGSTCVAALQAGRRYIGIELLEQYHRAGQQRLAAVRRAMQYPAAN
                     DEFPEAA"
     gene            9842..10063
                     /locus_tag="ISB30_002921"
     CDS             9842..10063
                     /locus_tag="ISB30_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990532.1"
                     /translation="MNYAGHEKLRAEVAEVANSMCDLRATLNGMEHRYRFDSDVLAER
                     LTRQTLFRINALFMAAYNEMLELDACFKD"
     gene            10074..10493
                     /locus_tag="ISB30_002922"
     CDS             10074..10493
                     /locus_tag="ISB30_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAP1990533.1"
                     /translation="MYGTCETLCRLLREQYPAETPLNLIVWSPADIEALADGMEYAVS
                     EQDTRAVLARMDAIPEEQRLESGVSAGAVMDLIEQVKEAVPAVMVPADLLETLLTTAE
                     QALWHREWTARDCNHPVPESVTRRLADAAKVRALLKK"
     gene            10547..11326
                     /locus_tag="ISB30_002923"
     CDS             10547..11326
                     /locus_tag="ISB30_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990534.1"
                     /translation="MQETTTLNALVMRRARDLIADYGWPDHTDVDQRDPVNKPGWISI
                     YVRLDAANIVHLLPLLCTGDVPADLQSAMTKISGTPAQIILSGSRYADAPQLPEDGTQ
                     IAFPWAGEWLTEPEIQAVTDCLSRAVRDVSRQVWEDARRIKAALNTRGETLFYRQTRN
                     FRLVVKENDMPCWLDDDDNLPVVLDAILNKGARYSSVEFFVISDKVDQILACGQMCDV
                     LRIPGEPPRRWMDLTLLHEVMAEARAEISLVRNALSAIRPV"
     gene            11731..12237
                     /locus_tag="ISB30_002924"
     CDS             11731..12237
                     /locus_tag="ISB30_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP1990535.1"
                     /translation="MRTTTTTPAVYVGTYHKYNCGSIFGKWFDLTEFDGREDFYEACQ
                     ALHADEWDAEFMFQDWEGIPSQFVSECSIDWDFIAAYKRAEEEGREAAFIAWAEYTGE
                     CDYDAFDDAYRGEAESEEDYAREMVEDNGLLNEVPEPLRSYFDFEAWARDLFSSGYVF
                     HDGYVFSN"
     gene            12280..12471
                     /locus_tag="ISB30_002925"
     CDS             12280..12471
                     /locus_tag="ISB30_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990536.1"
                     /translation="MKLAPETREILRQYKALINARRRDAGQRELTTAQVMDEICEYMT
                     CQCAVYIGGHFILQGGKGQ"
     gene            12664..12918
                     /locus_tag="ISB30_002926"
     CDS             12664..12918
                     /locus_tag="ISB30_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAP1990537.1"
                     /translation="MSKWNIASFSKEDQDKVSVDKVAAAVAWQERMNRPVVPELAERE
                     QPEHLRQYFRERLDVHRQNSQQLPRANAPEYHKPGDEQQK"
     gene            12953..13270
                     /locus_tag="ISB30_002927"
                     /pseudo
     CDS             12953..13270
                     /locus_tag="ISB30_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525572.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13446..13658
                     /locus_tag="ISB30_002928"
     CDS             13446..13658
                     /locus_tag="ISB30_002928"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990538.1"
                     /translation="MTQHQGLQHPYQDAPPLSRRGLRSLHPGENQRPPGCKGQRRFAP
                     ALSVVAAGSCSFLAWFFHPFESFRRG"
     gene            complement(13676..13825)
                     /locus_tag="ISB30_002929"
     CDS             complement(13676..13825)
                     /locus_tag="ISB30_002929"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990539.1"
                     /translation="MTFCGDLLNAHAGCYLHPFESRSPQGRKPARAMADVRGAVYRVP
                     FTLDS"
     gene            13896..14126
                     /locus_tag="ISB30_002930"
     CDS             13896..14126
                     /locus_tag="ISB30_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP1990540.1"
                     /translation="MSEALAVLPDDTFTREQAEVVAAQYTNVAIEDDQGTHFRLVVRQ
                     NGVMVWRAWNFEADTGKWLNRYIERYGIRKQQ"
     gene            14197..16206
                     /locus_tag="ISB30_002931"
                     /pseudo
     CDS             14197..16206
                     /locus_tag="ISB30_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211744.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            16249..16680
                     /gene="psiB"
                     /locus_tag="ISB30_002932"
     CDS             16249..16680
                     /gene="psiB"
                     /locus_tag="ISB30_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HAP1990541.1"
                     /translation="MRTILTPDVLKTMSCDEFEDWRDSGEDFRRELTHAVMRDLSCPE
                     NWDVNGEYRSEFGGFFPVQIRFTPSHGNYHIAVCSPGAISPAWMVVFVPASGRPFSVI
                     RTLSGYQPELVSHTVSLTARLDADGYSQASIISILAAEGAA"
     gene            16677..17405
                     /locus_tag="ISB30_002933"
     CDS             16677..17405
                     /locus_tag="ISB30_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HAP1990542.1"
                     /translation="MIPANLSLVPFSPERRAAMEAIAEVERRRSRGAVSTMYPYASAF
                     FRRLTGSTRITIREIHHFAPVLTARELRGSRDSWLSAIDALIESRGTRCWLPLPVGAG
                     QRLFPEVAFQQTERVRCQDALRDEKYTRQRHKERCQREQAYQALAGQAEIELAFHTPE
                     TVSSWSARWSGTELRQYDLEEMFWRWSERFPSLEPMERWTMESQPFWTVMAETNALAR
                     ESPGSVRQLERWMVPNKLTARSQV"
     gene            17787..18146
                     /locus_tag="ISB30_002934"
     CDS             17787..18146
                     /locus_tag="ISB30_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015493069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990543.1"
                     /translation="MRYWILVALLNGLFTLVIHYAAPEMLGGTAYYAGVAICALIALV
                     ITWRDPPGMLDNVLLFLLKWGIVASGLAFFIWGIGYKLEFWGDHNPDEIGGAERCSAS
                     GQWLGVLEAKVTNYTPA"
     gene            18772..19146
                     /locus_tag="ISB30_002935"
     CDS             18772..19146
                     /locus_tag="ISB30_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990544.1"
                     /translation="MNTQNVNVKTATKESSERWVKTSSLRTDGFVRNIHSRNPFDVIR
                     ADVVLARMEIQANRGCGLHYEIYESRLLGMAMHYLAELPLKDRPAFMGAAAKRGYMLT
                     TAEEERADGECADLMIELAADY"
BASE COUNT         4215 a         5116 c         5403 g         4471 t
ORIGIN      
        1 aatctgctcc atgcgggaga ggaaaatctc ttttcgggtc tgacggcgct tagtgctgaa
       61 ttcactatcg gcgaaggtga gttgatggct catgatgtcc ctctgggatg cgctccggat
      121 gaatatgatg atctcatatc aggaacttgt tcgcaccttc catcagtgac gatgatctgg
      181 tcagtgcgcc atcgtggccc gatattgcgc agcagctgca gcaccatatc ggccggcgcc
      241 cgctggttat tttcaatgct gagtttgata cgcgcattct gaagcagaca gcggcagcgc
      301 ataacgaccg tgccagctgg ctggactctc tgacggtgta ctgcgccatg cgacttgcag
      361 cggggtatta cggccccacc aatcgctacg gtaccatatc gctttccggc gctgtcagtc
      421 aggctgggct gagctgggcc ggggaggcac actccgccgt cactgacgca gttatgacgg
      481 ctcgggtggt aaacaatatt gccggatact ggcgtgagat ccagtgtgaa atgaatgatg
      541 gcgcagggag ataacgctgc tctctggctg gtcagacagc tgcaggtatt aattcaggag
      601 tctggaatgg aaaatatgat gaaaactatg cgtgagcatt ttcccacgag aacagtggtg
      661 atcgagcagt ctctggatat gtttggcgtc aaacgaaatg aggacgggac gttactcttc
      721 ccccgtcact ccgataaacg ttttcccatt gcacgtatcc gtaagcgact ggcggcttat
      781 ggctggcgag gcgaacgtct gaataaggag gtagcgcgta tcgtcgcggg ccctgatatt
      841 tacgctgaga gaaatgaata caacattatt ttcccttacg gatgggagaa gaagcagctg
      901 acgcgtgaga tgcggcgaat gtttccccgt aagacgggcc gacgtgtgct gattatgctg
      961 gatgaattac atcatttttc tgacttgcgg gagaagccgt gacgctgaca gaaaaatcgg
     1021 gccatctggc atggtgtgcc ctggtggcgc ttgcgctggc caggcaggag ggtggcgttc
     1081 tctcgccggc acaggaaaat ctctttctca tccgctggct ggcgactgcg ctgaagcaac
     1141 ggcgtttccc ccgggagata aatccggaca tcgagtggct gttgaaacag aggcgccagc
     1201 tgggcgtcag cgcgaagctg gccagcaagc tgaattacct ctggcgttcc tgcaccggcg
     1261 agttgtccga gcagaacgac ctgttccggc tgacgtatgc tctggaaaca gcgaaagata
     1321 tgcactggaa ctaccgtctg ctgagcgatc gggaatggtc aggccgaaat gctgtggcgc
     1381 tcagtgcggg cgtgaacggc atttatctct cacgggccaa cctcgatgtc gcttttgacg
     1441 acagcggccg acagataaat ccgcttacag cacgtctgac gggtaacgta gtgggggtga
     1501 tgaaggtgtt taatcgctgt ggctggcagg cagagcctga gtctggtgcc tccctgcccc
     1561 accagtattc tctgatggcc ggacagggag tgccaggaaa gggggattag tggcggatct
     1621 gcgctagttc gtcttcagtc agaccggtca ttttcatgac ggtattgcgg tcaattccgt
     1681 tctgcagcat ggttcgtgca attttaaggg tggcttcgcg ctcgcctttt tcaataccgc
     1741 gttgttcacc taactgcagg ccctgctgca gaccctgctg caggcccttc tcgatgccaa
     1801 tctgttcaag ctgttgtgcg atggtcatca gttcatcctc gtgttgcggc agacgctctg
     1861 ccagcgcccg cataaattca ggtgcaggcg cttcacctgc atttaccatg tagtttatca
     1921 gaacgcgcac ctgttcgggg gaaatccatt ccaccattac ccggggtagc ctgtccagca
     1981 gttccatcat gtcccgctgg cggatatgtt tcatcagcag ggtcagtgcg gccatccgtc
     2041 tgtggttcat tatctcatca tcatcgatga cggtgacatc aaccagcggg aatcctgtcg
     2101 tgtatacttt tccggcctgt tccgggttac tgaaacaatc cagccagttc gtggagaacg
     2161 ggtacggact ccggctgccg tggtaaaaca gaaccgggat gaccagcggc agttttttat
     2221 gacccgcttc aagatgacgt tgcatggcgg cgacagcata tctgagctga cgaaaggcca
     2281 tatttttatc cgccgaactc tggtgttcca gaagcagatg gatgtacccg tcatcgccgg
     2341 tggtggtttt catgctccag agaatgtcgc tgacgtactg acgtaagtcg tcctcaacga
     2401 aggtaccgga ctccagcttc agcgtggtca aatcgcacag cgcgcgtaat tcagccggga
     2461 gatgtaacat catgaagtca cgggcgacat cgggattcgc caggaactgg cgaaatgtcg
     2521 cgtcatgggg tgtgggtgta cctggtttct tcttcatcag tcctgggctc tgaaaaatga
     2581 cccggagatg ttatcacagc ccgggaaaag ctaatattgg cggtgaacgg aattgccatt
     2641 ccttcagggg accgttccgg ggtggttcgg tccccttcct aaaccatctt tactcatcat
     2701 caattactgc cttagcaact ttcttccctc tctcagatat tctaaagcct cttctctccg
     2761 tccttctgtg gcagctgtat ataatgtcgc gataccgctg acagcctgat ggtgggttat
     2821 actgccttta ctgaaatcat ccagaacagt acaaaggaat tcctccaaca actcctgttc
     2881 tctttgagat attctttcag ccatattcgc tccataagct taattgtttg ttctgaacct
     2941 aacgtgaata agtatcgtca atcagtatcc tgcttatgat tgccgttaca gaccctgatt
     3001 actgtcatct ttgatacgcc agccaggcgg gctgtttcac gcagtgattt tccgtgggcc
     3061 agacgcagag tacgtatcag ttcatgtttc tgtgcatcgg ccaccctgcc ccggtatttt
     3121 ccttcagttt tcgctttaca gattccctct gcctggcgac gtcgacggtc ttcgtagtct
     3181 tttctggcaa tagcggcgag catatccagc atcatgccat ttacggcctt cagcatgctt
     3241 cgggtgaagt catcacttac tgtgctggat agcgccagat ggctggttgg taaatcaaga
     3301 ctgacgatgg ccagctgctt gtcggtgatt tgctttctga gtgtctccca gcctgcatca
     3361 tccagtcgag taagcctgtc gacctgttca atgagaatca catcaccagg ttccgcatca
     3421 ccaagaagcc tgatcagttc aggacggttc attgtcgtac cggagacatt gtcgatatac
     3481 cagccagcaa tacggtggcc attttggttt gcaaatgcct tcagtgcatt ttttgcccgg
     3541 ctggcattct gctctgaagt ggatgcccgg aggtaaccaa aaacgaacat attttgtcct
     3601 tctggtaact tttaagtggt cttgcttatg atggtatcac ttatgtggta acaataatat
     3661 tcgccggtat tttttcgggt ggtatatata gagcataccc caatgtgaca ggctgacgcg
     3721 cccacggagc ttgtccaacc cctcgcctgc tcagatgcaa gctctgaaca gactcacggt
     3781 tttttctttg agtacagcct cagttgtctt tttgagctac gtgataggta ggtattaacg
     3841 tacaataatt taatgtcgta tcattatatg ttgtacgtta ttatttattg tgtacaatgg
     3901 cgtgagcttc tacgccatca atgtgcaagg attccatctt taggggacaa aaatgaaacg
     3961 cgattacggt agtgtgggta ccatagctct tagagcaagc gctttacttc aggctatgag
     4021 tagggatatt gaggaacaaa ggaaagaatt taatctgaca gattatcatc agacatatac
     4081 tcgtaacgct gtcgcaaaac taccaaagct gagccggcgt atcgtagaac tggccatgaa
     4141 agaaatggaa gaagacggct atatatttaa taaaaagcag attggtaacg tcgaacaata
     4201 tgctctgacg ataaagaatg ttatcgatat ctacgcccac cgacagatac ctaaatatcg
     4261 cgatatccat aaagaaccct acgttatttt cgttgtaaac cttaaaggtg gtgtatcaaa
     4321 aacagtcagt acagttactc ttgcacatgc gttgcgtgta catcaggacc tgctgcggca
     4381 tgatcttcgc atccttgtaa tagacctcga cccacaggcc tccagtacta tgttcctgga
     4441 tcacacccat agcattggta cagtccttga aacagctgct caggcaatgc tgaatgatct
     4501 cgatgttgaa acattgcgag aagcagttat tcgccccacc attattcctg gtgttgatgt
     4561 catccctgct tctattgatg acggctttgt ggcgagccag tgggagtctt tagtagcaga
     4621 acatttacca ggacttaaac cttctgaagt cctcaggaaa acaattattg accgtatcgc
     4681 tggtgactat gattttgtct tcatagatac tggcccccat cttgatccgt tccttctgaa
     4741 tggtctggca gcgagtgatt tgcttctgac ccctactcca ccggcacagg ttgatttcca
     4801 ttcaaccctt aaatatctga cacgtttgcc tgaaatgctg gagcgtcttg aagacgaagg
     4861 tgtggaacca cgcctgagcg cgagtatcgg tttcatgtca aaaatgacta ataagcgtga
     4921 tcacgaaact tcgcatagcc tggcccgcga agtgtatgca agtaatattc tggactcgtc
     4981 actgccacgt ttggatggat tcgaacgatg tggtgaatct tttgacacta ttattagtgc
     5041 taaccctgtt tcataccctg gtagtgctga agcgttgaaa aaggcacgta ctgaagcaga
     5101 acggtttacg aaagcagttt ttgaccgcat tgaatacatc cggggagagt ctaaatgaaa
     5161 aaggttttag ctcgtgggcg agttctcggt aatagcaatt cagagtttgc ccgtatgctt
     5221 gaaggtgacg gagatgtcaa aacgtttact cttaaatccg gcaagcaggc caaatttgtc
     5281 aaaacggttg tattaagcgg agagattgaa tcaaagacgt tcgttgatgc atcggtcaat
     5341 ggacgagatc aggctatgct tacgcgtgag tccgttagtg atatttcccg gacgataaag
     5401 ctgcagcaat tcttcccggc tattggacgg gaggttaatg gacgaattga gatattagat
     5461 gggacccgac gccgtgccgc ctgcatattt aataacgtta aattcgaaat cctagtaact
     5521 aaagatgata tatcactcgc cgatgcacgg cagttagcgc aagatatcca gacagctaga
     5581 gaacatagcc ttcgagagtt ggggaaacga cttgaagtca cctatggaac tagcatgacg
     5641 aaagaagaga ttgcgttgaa agagaatctc tctccggcga aagtgactcg cgcgtttcag
     5701 gccgcagcag tgccagatga aatggttgca gtattcccgg tgataaatga tatttctttg
     5761 tcggattatc agtttttgct aaaactggct gaagaagcaa acaacaagca aacatccgta
     5821 acagaactga tggaaaaagt tcaacatcgg ttgaagacca tgccagatta tccggcaatt
     5881 gataaaagca aaattctggc ggctatcagg gttgagagca aatcgttgac aacccgccct
     5941 accagaacgg ttcaaactga gaagctgaga gaattttcag atcgcaatca gtttgccaga
     6001 aagaaaactg attcaaagaa gcgactggtt gtttatgagt tttcccgcat ttccgctgag
     6061 gcacagtcgg agattgatga tgcaataaaa cgtattctga aaagacttcc tgagtcaagt
     6121 gagtaaggga aaaggatgcc ggagagcatc ctttttttat gtttttcacc atgccgattt
     6181 caatgttatt attatgattg taaaggaaaa ttttaaattc tttcacactg aaatcaccaa
     6241 gtttttcaac cttttcgtga ctcataattt cgctactggc agttcagacc agcgagttgt
     6301 ataggccggt gaaagcatct cccgtttcat ttgccactca ggtgcgatac ctcgtcccgc
     6361 aaaccatact ttccccagac cggaatggtt aataccgtcc atgactttca tcagctgatt
     6421 actgttgctt cggggctgag tatcatcgaa caggttcaac tgcgaaaccc cggtaggagt
     6481 gaaatcattg agcatgcaac ccgcttttgc atatcgatgg ccattcaccc agatccgatc
     6541 cagtgctttc accgctgcgg ctatgatgtc cctggtatcc tgcgtgggag tcatcaactt
     6601 ctcactggcc acattcccgt aatacggttc gttcaccgcg aacggtgacg tcttcacaaa
     6661 tacagcaatg tgcctgcaga actgtcgctc gccgcgcagt ttttcagctg cacgctcagc
     6721 atactgacaa actgcctggc gcatggcttc atacgtcgtg acacgctccc caaaggaccg
     6781 gctgcagaca atctgctgtt ttggcggcgg cgcttcttca agcgaaatac agctttctcc
     6841 gttgagttca cggacggttc tctccagcac aacgctgaaa ttcttcctga taaatacagg
     6901 gttcgcgcgc gccagctgca gtgccgtagt gatgcccatc gtattaagcg tttttgagat
     6961 cctgcgacct actccccaga tttcttccac cggctgtcgt gagagcagtt tatccgttcg
     7021 ctttggattg cctgaagtca gagcaagcac gcccccaaac tgccgccatt ccttagacgc
     7081 ccattgtgcg cttttagcca gcgttttggt cggccccatc ccgacgccga ttgtcagccc
     7141 cgtaccggag cgcacgtgct cgcgcagctg ccggccgaag tcctcataat tgatgcagct
     7201 gtctataccg cggatatcca gaaacatctc gtcgatgctg tactgctcca cgcgcggcgc
     7261 cagttcttcg agatgagaca taactctgtt cgacatagac gcatagagct cgtaattgca
     7321 cgagaacgtg atgaccggct ccgggaattg tgccgtcttc agctggaacc aggggacgcc
     7381 cattttaata cccagccgtt tcgcttcggc actgcgcgcg atgacgcagc catcattgtt
     7441 actgagcacc acaacgggcc tgttacgcag gtccggacga aatactttct cacagctggc
     7501 gtagaagctg ttgacgtcag ccaaagcgaa catcaattcg taccccgcgt cttatgtatg
     7561 aatgccatca ccacgccgaa tatctgcagt tcttccggat aaagagtggg gtaggcggga
     7621 ttcatcggca gcagggcgat acggggttta agctgcaggc gcttaacggt gaattcgcca
     7681 tctgtttccg cgatgacaat atccccctgc atgggttttt cagctttgtc gacaaccatg
     7741 agatcgccgg agtgcaggcc catatctttc atcgaatcgc caatggcccg gacaaagtag
     7801 gtcgcgctgg ggcgacggat acagtaggcg ttcagatcca gttcgtcttc ggtatagtcc
     7861 gcggccggtg acggaaaacc agccggacag cgctctgtga aaagtggtgc ggtggacagt
     7921 gctggctctg atgcgggagt gacaaaaagg atcatggcat acctcttaaa atacctgttt
     7981 ttacatacag tgttatttaa gatatttccc gtcctgcaag gagaattttt cctgttcctg
     8041 ccaggttatt gttctggcag ataaaacagg cagtgggtta tccacagaaa cggtgcataa
     8101 ccctgtgagt cgatgccggg tatctcaccg tctgctaagt cagtggctct ataagttccc
     8161 ggccctggtt cccgggattg ccaacagccc ggctgacggg gtgccatgtg aaatcttcgg
     8221 cgggaagact gccctcacgg gcaatgtctt ccgcctcttt accggttgtg ttttcgctga
     8281 gccatttacg tgcggcatcc ggtgagtaca cgagtggtcg ccggtcgtga atgtccagca
     8341 gttcctcatc cgcagcggcc gtgacgatca ggaatccttc ctggtcattg ccgttctcaa
     8401 aaggtggttt cccgatggca gcgagcagga gcggatttcc gtccttacgg taaatgaaaa
     8461 acggctgttt cacgccgttt tgctgtttcc actcaaacca gccgtcagca aaaacaatgg
     8521 cccgtccgtg ctcccagagt ggcctgaaca tccggctgcc ggccgcagtt tcacttctgg
     8581 cgttgatgag agcgggcttg ttccaccact ccggcgcata accccagtga acaggatcaa
     8641 gatgcagctg cgagcggcgt tcactcagca gtaatacacg ggttccgggc gcaacgttgt
     8701 agcggccgac gggctccgga tcatacggga tgtcccgttc tgcttcttct ccgagaaagg
     8761 ccaggtactc ctcgcgggag ttgtactgcg taaaccgacc gcacattatc gtttctccgg
     8821 tgtagatttc gcatgcgaaa tctgtgtttt catcagttca gaaaataggc cgtttttcag
     8881 gttcctgcca tcgtgtggcc gccgtgccgc tactgtagcc gggccgcttc gaatgcaagg
     8941 gttcgctctg ccggtgtcct cacccggtcg cagtttattt caaaggcttt acccgctgtc
     9001 ttcccgtcgt tttccgtctc aattttcgcc gtcaaaccgt ccagccagcg tgggcttttt
     9061 cactgcgctg cggcttgcgg caccgcccct gcattctcca cttttcgccc ggctgtcgta
     9121 tcggcacggc gtaactggcg ccgctttgag aaaggagaac tactatgtcc cgatttatcc
     9181 agggtaattg cgtccatatc atgtccggct ttccggataa tgctgtcgat ttcattctca
     9241 ccgacccgcc atatctcgtc ggttttcgtg accgtcaggg gcgtaccatc gccggcgata
     9301 aaactgatga atggctgcaa cccgcctgcc atgagatgta tcgcgtgctg aaaaaagacg
     9361 cgctgatggt gagcttctac ggctggaacc gcgtcgatcg ctttatggcc gcctggaaaa
     9421 atgcgggatt cagcgttgtc ggccacctgg tgtttaccaa aacctacaca tcgaaggccg
     9481 catatgtggg ctatcgccac gaatgcgcct acatccttgc aaaaggccgt ccgcccctgc
     9541 cgcaaaaccc gttgaatgac gtaattgcgt ggaaatattc aggcaaccgg caccacccga
     9601 ccgaaaaacc cgtaaccagc ctgcaaccgt tgattgagtc cttcacacat cccggcgcga
     9661 ttgtgctcga cccctttgcc ggcagtggct ccacctgcgt ggccgccctc caggctggcc
     9721 gtcgttacat cggtattgaa ttgcttgagc agtatcaccg ggccggacag cagcgtctgg
     9781 ccgccgtccg gcgcgccatg cagtacccgg ccgctaacga cgagttcccg gaggccgcgt
     9841 aatgaactat gcaggacacg aaaaactccg cgcagaagtg gcggaagtcg ccaacagcat
     9901 gtgtgacctg cgggcgacgc tgaacgggat ggagcaccgc tatcgctttg actctgatgt
     9961 gctggccgaa cgcctgaccc gtcagaccct ttttcgcatc aatgccctgt ttatggcggc
    10021 atacaacgaa atgcttgagc tggatgcctg ctttaaggac taaggagaaa aacatgtacg
    10081 gaacctgtga aaccctctgc cgcttgttgc gtgagcagta tcccgcagaa acccctttga
    10141 acctcattgt ctggtccccg gcggatatcg aagcactggc cgacggaatg gaatatgccg
    10201 tttcggagca ggacacaagg gcggtactgg cgcgtatgga cgccattccg gaggaacaac
    10261 ggcttgagtc gggcgtgtct gccggtgcgg tgatggatct gattgaacag gtgaaagagg
    10321 ccgttccggc ggtgatggtg ccggcggacc tgcttgaaac cctgctgacc actgccgaac
    10381 aggcgttatg gcacagagaa tggaccgccc gtgactgcaa tcatccggtc ccggaaagcg
    10441 ttacccgtcg cctggctgat gcggcgaaag ttcgcgcctt actgaaaaaa tgaaatcaac
    10501 acgccgcccg ggcggcgtgt actgaccccg ttcagaggaa cccattatgc aggaaaccac
    10561 cacgttaaac gcccttgtta tgcgccgtgc gcgcgatttg attgctgatt atggatggcc
    10621 tgaccatacc gatgttgatc agcgtgatcc ggtcaataaa ccgggctgga taagcattta
    10681 tgtccgtctg gatgcggcaa atattgttca tctgctgcct ttgctttgta ccggtgacgt
    10741 acccgcagat ctgcaaagtg ccatgacaaa aatatccggg acgccagcgc agattatttt
    10801 atccggcagc cgctatgccg acgcgccgca gcttccggag gacggaacac agatagcttt
    10861 cccctgggcc ggggaatggc tgacggagcc ggagattcag gctgtaacgg attgcctgtc
    10921 ccgcgccgtg cgggatgtat cccggcaggt atgggaggat gcgcgccgga taaaggcggc
    10981 actgaacacg cgcggggaaa cgctgtttta tcgccagacc cgaaacttcc gtctggttgt
    11041 gaaggaaaat gatatgccct gctggctgga cgatgatgac aatttgccgg tggtgctcga
    11101 tgccatcctg aacaaagggg cacgctacag tagcgtcgag ttctttgtta tcagcgacaa
    11161 agtggatcaa atcctggcat gtggccagat gtgcgatgtg ctgcgtattc cgggcgagcc
    11221 tccgcgccgg tggatggatt taaccctgtt acatgaggtc atggcagaag cgcgtgcaga
    11281 aatcagtctt gtccgcaatg ccctgtcagc aatccggcca gtttagcgaa cagcaggggg
    11341 cggcggcccc ctgaatctgg cacgaaggat aaggatgtcg ccggctccgc ttttacggct
    11401 cttcctccac gttgttccgg cttcgcgttg agtaacgtcc cgtgcggcct gcggcaaacg
    11461 ccgtgcctct actgtagccg ggccgcggct gatgcaaggg aagcgtaaac gccgtcacct
    11521 tcacccggtc gcagtttatt tcatacgtta tccgccgtct tcccgtcgtt ttccggctca
    11581 gttttcgccg tcaaaccgtc cagcctgctc ctgctttttt actgcgctgc ggcttcggtg
    11641 acgcccttgc atcctccgct tttcgcccgg ctgtcgtgtc ggcacggcgt aagccggaac
    11701 tcagacgaca tcgactaggg aggatttttt atgcgcacta caaccaccac acccgctgtt
    11761 tacgtgggca cgtatcacaa atacaactgc ggcagcattt tcggcaaatg gtttgatctg
    11821 acggaatttg acggcaggga ggatttttac gaggcctgtc aggcgctgca tgccgatgaa
    11881 tgggatgccg agtttatgtt tcaggactgg gaaggtatcc cgtcgcagtt cgtctctgaa
    11941 tgctccattg actgggattt tatcgccgct tacaaacgcg ccgaagaaga gggcagggaa
    12001 gccgctttta tcgcctgggc ggagtatacc ggcgaatgtg actatgacgc gtttgatgat
    12061 gcgtaccgcg gcgaggcgga aagcgaggaa gactatgcgc gggagatggt ggaggacaac
    12121 ggcctgttaa atgaggtgcc ggagccgttg cgcagctatt tcgattttga agcctgggcg
    12181 cgtgacctgt tcagcagcgg ttatgtgttc catgacggct atgtcttcag caactgatta
    12241 atgtccggcg ggcaaacccg ccggttaagg agaaaacgta tgaaacttgc accggaaacc
    12301 cgcgagatcc tgcgacagta caaagccctg attaatgccc gccgacgtga tgccggtcag
    12361 cgggaactga ccaccgcgca ggtgatggat gaaatctgcg aatacatgac ctgtcagtgt
    12421 gccgtgtaca tcggcggtca tttcatctta cagggtggaa aaggccagta agtatgatgg
    12481 caggcgtccg ggccggaggg cgcctgtcag cccgcgcgaa aaaccgggca ggctgcgcct
    12541 gccggagaga aacttcgccg cgcctgcggc gccgcgcact ctcctctcca ttgtcagtcg
    12601 ctgttgcccg ggaatgtgcg tattatcagg gatatcttcc tgtaaagcag accggtgatg
    12661 catatgtcga agtggaatat cgcctctttc tcaaaagaag atcaggataa ggtctcggtc
    12721 gataaagtcg ccgctgccgt tgcctggcag gaaaggatga acaggcccgt ggtgccggag
    12781 ctggctgaac gggaacagcc tgaacatctt cgccagtatt tccgtgaacg gcttgacgtt
    12841 catcggcaga acagtcagca gctgccgcgc gcaaatgcgc ccgaatatca taaacccgga
    12901 gatgagcagc agaagtgatt cttatagtca gtgtgtccag ggaggaaatt ttatgcgtat
    12961 gtctctgatg tttgcggccg tgactctttt aaccggtatt aacctgttgt ttattccgtt
    13021 tcagcctgta ccggctctat gggggggaat cttggccgtt gcttttatcc tgattaatgt
    13081 cgcggcggat aaactgcgtc tctgaggtgg cgttgcatgg attatcgttg ccggcatcat
    13141 cataatgttg atgctgaagg ggaaggaacc gggggacgaa ttaagagtgc tgatcaccgt
    13201 ttttgctttc ctggcttcgg cctggtatag cgttgtgcag tacagaaaag aaaagcattt
    13261 tcgctcctga tgttcccggc tggcatcaag ctggatgatc gtcagccatc attttagccg
    13321 catgtaatcc attcttatcc tcccgttttc tgttttctgc ccgttcccgt tttcattgcg
    13381 ggcgtccggg cgggctgtcg tgaacggagc cggtaaaccg tctccgccct tcgggcttcc
    13441 atcccatgac gcaacaccag ggcttgcagc atccttatca ggatgcgccg cctctgtcgc
    13501 gccgtggcct gcgctccctg caccccggcg aaaatcaacg gccgccgggc tgtaaggggc
    13561 aacgccgttt cgcgcccgcg ctttccgtcg ttgcggcggg ttcctgctca ttccttgcct
    13621 ggttttttca cccttttgag tcctttcgcc ggggctgaga caagcacagc cgctgtcagc
    13681 tgtccagggt aaatggcacg cggtaaaccg cgccccggac atccgccatc gcccgcgctg
    13741 gtttacgccc ctgcggcgaa cggctctcaa acgggtgaag gtaacaacca gcgtgggcgt
    13801 tcagcaggtc cccgcaaaac gtcaaaaccg cggccccggc aactcaacgg gtgatggttt
    13861 tttaaaagca aaacaggttc agaaggaggg gcaacatgag cgaggcgctg gcggttttac
    13921 ccgacgacac ctttacccgc gaacaggctg aggtcgttgc ggcgcagtac accaacgtgg
    13981 caattgagga cgatcagggt acacattttc gtctggtcgt tcgtcagaac ggcgtaatgg
    14041 tctggcgcgc gtggaatttt gaagcggata ccgggaaatg gctcaaccgt tacatcgaac
    14101 gttacggtat tcgtaaacag cagtaaataa gaaggtgccc tgccggagcg gtaactccgc
    14161 agggccggac aatcatcaat cagtgaggta tcaactatgt cagtaaccga tgttaaagca
    14221 aaagcaccca aaaaagcgag cagcaaaaaa attacgaagg cacaggaggc tgccctgaag
    14281 atcgcccttg aggccgctgc ggtcgagtac gttccgctgt ccgatctcgt taaatccccg
    14341 ctgaacgtgc gctccatccc ctattccgtg gacagcgttc gcggtctggc cgactccatc
    14401 gagacgctcg gactgctgca gaaccttatt gttcacaccc tcgcggacgg acaatccggc
    14461 gtggccgcag gtggccgccg attaactgcc ctgcaactgc tggcgcagga agggcgtctc
    14521 gctgctgacc ataccgttat ggtgaagcgt gtttccgacg acattgccgc ccttgcctcc
    14581 gtcgccgaga acgagcagcg cgccgccatg catcccgccg agcagattgc aggtttccgt
    14641 acgctggcag ggcagggcaa aacccccgcg cagactggcg acgcgctcgg cttcggctcc
    14701 cgtcacgtgc agcgcatgct gaagctggca aacctcgccc cctccctgat ggagaagctt
    14761 gcacaggacg aactgaccgt tgagcagtgt caggcgctct gtctcgagga cgatcctgcc
    14821 cgtcaggtcg aggtattcga aaacgtgaag gccagctagt caaacgcgcc cgcacacctg
    14881 attaaacgcg ctattaccga aaccgagatg cgcaccgaca acgccaaatt tcgttttatc
    14941 gggcgcgatg cctacgaggc ggcagggggt tacgtccgtg aagatctgtt cagccaggac
    15001 gagggcgacg gtacggctga cagcgtgctg gttgagcgtc tggtgcagga aaagctggag
    15061 cgtatcgcgc aggacattca gcagcgggag ggctgggcat ggagtcgcgg acgcgcagcc
    15121 cgcatctggt accacggcga agacggtaag gagttcgtgc agcctgctga acccgatccg
    15181 gtgtacaccc ctgagcagca gcagcgtctt ggtgcgctgc gggagcagta cgatacatat
    15241 gacagcgttt gcgacgagtc agatgcgatc gaagcggaca tcctcgccat tcaggcggcg
    15301 gcagaagcca gcgcgtggac tgacgacatg aagtcaggcg cgggagtgat ggtcagcctg
    15361 tacgaagggc aggtgtacgt gcagcgcggt gtgcgcctga aagcggatat gcctgaagaa
    15421 accgtaacca gcagcgtaac ggtgccattc acctcacacc agcccgacgc cgcagagggg
    15481 atcagtgttc cgctgctcac gaaaatgacc tccgaacgta cgctggcagt ccaggcggcg
    15541 ctgatgcagc agcccgaaaa ggcggtggcg ctgatggtct ggcgcatgtg tacctgcgtc
    15601 ttctcgggct gcctgaccac gacgcatccg ttccgtatca gcctcactgt atctcacggc
    15661 agcctgacgg agaacgcccc gtccggtaag gatggcgcag cgtttaacat cctgatgacc
    15721 gaacgggcaa ggctgaaagc cctgctgccg gaagggtggg agaaggactt caccaccttc
    15781 tttgcccttg acggcggggt gttgatgtcg ctgatggcct tctgcacggc ctgttccgtt
    15841 gacggggtgc agacccgcga tatggggcac acctcgcgca gtaccctcga tgcggtcgag
    15901 gcggcaatcg gcttccacct gcgcgactgg tggcagccga cgaaggacaa ctacttcggc
    15961 agcctgaaac atccgcagat tgtcgcctcc ctgaaagagg cggggctgac gggcgcggcg
    16021 ggtgacgcgg agaagatgaa gaagggcgat gctgcagcgc atgcggagca ctttatgcag
    16081 cacacccgct gggttccggc atggctcaaa ggaccggagt cagcgggtga atccggtgca
    16141 gacgacgcgg cttcagatac cgacagccct gacaacgaca ccaccaacac ggcacacgcc
    16201 gcctgataac ggagcgccgc cctgcggggc ggcgataagg agatacccat gcgcactatt
    16261 ctgacccccg atgtactgaa aacgatgtcc tgtgatgagt ttgaggactg gcgggacagc
    16321 ggcgaagact tccgccgcga actgacccac gccgtcatgc gtgacctgtc ctgccctgaa
    16381 aactgggatg tgaacggcga gtaccgcagc gagttcggcg gcttcttccc cgtacagata
    16441 cgctttaccc cctcgcacgg caattaccat attgcggtgt gcagtcctgg tgccatcagt
    16501 ccggcgtgga tggtggtgtt cgtgcccgcc agcggtcgcc cgttctccgt cattcgtacc
    16561 ctgagcggct accagccgga gcttgtcagc cacaccgtca gcctgacggc gcgtctcgat
    16621 gcggacggat acagtcaggc cagcatcatc agtattctgg cagcggaggg cgcggcatga
    16681 tacctgcaaa cctgtcactg gttcccttca gccctgagcg acgcgccgcg atggaggcca
    16741 tcgcggaggt tgaacgccgg cggtcgcgcg gcgcggtgtc cacgatgtat ccgtatgcca
    16801 gcgccttttt ccggcggctc acgggcagca cgcgcatcac tatcagggaa atccatcatt
    16861 ttgcgccggt gctgacggcg cgggagctgc ggggcagcag ggacagctgg ctcagtgcca
    16921 tcgatgcgct gattgaaagt cgcgggacgc gttgctggct gccgcttccc gttggtgcgg
    16981 gtcagcgcct ctttccggag gtggcctttc agcagaccga gcgggttcgc tgtcaggacg
    17041 ccctgcggga tgaaaaatac acccgacagc gtcacaaaga acggtgccag cgtgagcagg
    17101 cgtaccaggc gctggcgggg caggcggaga ttgagctggc attccatacg cctgaaacgg
    17161 tcagcagctg gagcgcacgc tggtcgggga ctgaactgcg ccagtacgac ctggaagaga
    17221 tgttctggcg ctggagcgag cgtttcccgt cactggagcc aatggagcgc tggacgatgg
    17281 agagtcagcc tttctggacg gtgatggctg aaacgaatgc gctggcaagg gaaagtccgg
    17341 gatctgtccg gcagctggag cgctggatgg tacccaacaa gctgacagcg cggagtcagg
    17401 tatgaagtgg cgttactcgc tgcgctggaa gcgtcccggt ccctgtcccg gtgaacctga
    17461 actggcgtca gaagtggtgg aggccgggaa gccagcccct gagtccgtga tgtcactgtg
    17521 ggtggcagga gccggatatg ctgtctgcgt tgattttctg tatgaacggc cgatcaggcg
    17581 ctggagcgat gagcgaaagg cggccacgcg gcgacggaat ctggcgcgtc gtgttaacag
    17641 gatcgcgccc ctgtttgcag atgaactcat tgagcgggaa ctgacggtgc gaccggatta
    17701 ttttcgggga aagtcgcctc actgagtctg atatcatatc ctgccagaat acggtcggca
    17761 gggtgcttaa aagggataat ttagagatga gatactggat actggtcgcg ctgttaaatg
    17821 gcctgtttac gctggtcata cattacgcgg cgccggagat gttgggaggc acggcgtact
    17881 atgccggggt ggcaatctgt gccctgattg cgctggttat cacctggcgc gatccgccag
    17941 gtatgctgga caacgtgctc ttgtttctcc ttaagtgggg aattgtggct tcaggtctgg
    18001 cgttttttat ctggggtatc ggatacaagc tggaattctg gggagatcat aaccccgatg
    18061 aaattggcgg tgctgagcgg tgttctgctt ctggtcagtg gctgggtgta ctggaggcga
    18121 aggtaacaaa ctatacgcca gcgtgaggca ggaaagtaaa aagagcggtt atgcctgcgg
    18181 tataactgct cttttatatg gtcctcacgc tttgtaattt cctgctgtga atcacctctg
    18241 aagaacgaac gctgcgaatt atggtggcag ttgtctgtat catcttttca aatttgcctg
    18301 ctgtctgacg gggtcactgg ccatgactgc gggcgacctc cctgacggga accggggctg
    18361 tcgtaaacgg agccggtaaa ccgtctccgc cctgacgggc ttccatccca ctgtcgcaac
    18421 gtcaacaccg gctcgcagtg accgttccgg tcacgccgct ttcaccatgc cggcgataaa
    18481 gccgccggca ctcaggtgac gccacgtcgt tccgccgtgt cgggtgtgtt catcctctcc
    18541 ccgtacctct ggcgagccct ctcccgttaa gtccccgggc ggagggcttg tcgtagcacc
    18601 gtcgtgtcgt catccgaagg gtgaaatgca ctccgccaaa actttttgcg agggaccgca
    18661 aaaactttag tctccgccct tctcctgccg ccccgccgtt gctcctttgc cctttgccgc
    18721 cagggaactc accgagagat ggataaacca ccagacagga ggaaaagcag gatgaacaca
    18781 cagaacgtca acgtcaaaac cgccaccaaa gaatcttccg aaagatgggt gaaaacctcg
    18841 tccctgcgta ctgatggctt tgtgcgcaat atccattccc gtaacccgtt cgatgttatt
    18901 cgcgcggacg tggtgctggc ccgtatggaa atacaggcta accggggatg tggcctgcac
    18961 tacgagattt atgagtcccg tctgctgggt atggctatgc attacctggc agaactgcca
    19021 ctgaaagacc gtccggcgtt tatgggggcg gcagcgaaac ggggttacat gctgacgact
    19081 gcggaagaag aacgtgccga tggtgaatgt gctgacctga tgatcgagct ggcggcggat
    19141 tactgattac ttaaccggct ggcggcaggg gatgccgcca gtcacaccgg agaaacatta
    19201 tgagc
//
LOCUS       DACADF010000085        18990 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.086, whole genome shotgun sequence.
ACCESSION   DACADF010000085 DACADF010000000
VERSION     DACADF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.086"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            209..979
                     /locus_tag="ISB30_002936"
     CDS             209..979
                     /locus_tag="ISB30_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAP1990545.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            970..1443
                     /locus_tag="ISB30_002937"
     CDS             970..1443
                     /locus_tag="ISB30_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP1990546.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1550..2089
                     /gene="dnaT"
                     /locus_tag="ISB30_002938"
     CDS             1550..2089
                     /gene="dnaT"
                     /locus_tag="ISB30_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAP1990547.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2092..2829
                     /gene="dnaC"
                     /locus_tag="ISB30_002939"
     CDS             2092..2829
                     /gene="dnaC"
                     /locus_tag="ISB30_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAP1990548.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2878..3372
                     /locus_tag="ISB30_002940"
     CDS             2878..3372
                     /locus_tag="ISB30_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAP1990549.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3626..5917
                     /gene="opgB"
                     /locus_tag="ISB30_002941"
     CDS             3626..5917
                     /gene="opgB"
                     /locus_tag="ISB30_002941"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAP1990550.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLTWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6057..7079)
                     /locus_tag="ISB30_002942"
     CDS             complement(6057..7079)
                     /locus_tag="ISB30_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP1990551.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEVVGLGKNVADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7218..8132
                     /locus_tag="ISB30_002943"
     CDS             7218..8132
                     /locus_tag="ISB30_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP1990552.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFEQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENMRYAH"
     gene            8349..9710
                     /locus_tag="ISB30_002944"
     CDS             8349..9710
                     /locus_tag="ISB30_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1990553.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9759..11423)
                     /gene="tsr"
                     /locus_tag="ISB30_002945"
     CDS             complement(9759..11423)
                     /gene="tsr"
                     /locus_tag="ISB30_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAP1990554.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLHDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVMPAAPRKMAVADSGENWETF"
     gene            11800..13950
                     /gene="btsT"
                     /locus_tag="ISB30_002946"
     CDS             11800..13950
                     /gene="btsT"
                     /locus_tag="ISB30_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAP1990555.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            14000..14203
                     /locus_tag="ISB30_002947"
     CDS             14000..14203
                     /locus_tag="ISB30_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAP1990556.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            14214..15170
                     /gene="yjiA"
                     /locus_tag="ISB30_002948"
     CDS             14214..15170
                     /gene="yjiA"
                     /locus_tag="ISB30_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAP1990557.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVISTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(15204..15506)
                     /locus_tag="ISB30_002949"
     CDS             complement(15204..15506)
                     /locus_tag="ISB30_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HAP1990558.1"
                     /translation="MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(15493..15780)
                     /locus_tag="ISB30_002950"
     CDS             complement(15493..15780)
                     /locus_tag="ISB30_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HAP1990559.1"
                     /translation="MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIEN
                     GEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIISARKADRKERSRYEHG"
     gene            15977..17143
                     /locus_tag="ISB30_002951"
     CDS             15977..17143
                     /locus_tag="ISB30_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAP1990560.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIRDQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRGMVTREGMSYCITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            17207..18826
                     /locus_tag="ISB30_002952"
     CDS             17207..18826
                     /locus_tag="ISB30_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004720069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP1990561.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPANTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTEDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEALGYGE"
     gene            18816..>18990
                     /locus_tag="ISB30_002953"
     CDS             18816..>18990
                     /locus_tag="ISB30_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP1990562.1"
                     /translation="MGSKWCIYRLGDIAEFSYGKMPKKEFVGTGDYVIFSGYKYTESY
                     PEKNTSAGELIVVA"
BASE COUNT         4619 a         4984 c         4745 g         4642 t
ORIGIN      
        1 caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt
       61 cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta
      121 acctacctca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
      181 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacgcg
      241 gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga
      301 tgagctttcc tcacgactgg gtcgggcgtt gggaatggac agcgtcgaaa gttctatctc
      361 ttcgaacgcc atagtgctga caactattaa agatgggcaa tgcctgacat cgacacgaaa
      421 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
      481 tgcggagcat catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
      541 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
      601 taaactcaat aacggtggct gggatggtgc cgtcatcacc ttctttgcca gtacgaccgc
      661 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccacagatca acttttgcct
      721 taccgctttc gccgccacca ccatttccgg attgcttttg caactcccca ctttcagcga
      781 tacacccacc attgcgatgg ccgccagcgt tctgctgctg gtgccgggct ttccgttgat
      841 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
      901 cgccagtctg ctgacgctgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
      961 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     1021 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgtgcgggc gttacgctgg
     1081 tgtgcgctgc ttggcgcgat tggccatggt tcacgaatga tcttaatgac cagcgggctg
     1141 aatattgagt ggtcaacttt tatggcttct atgctggtcg gcaccattgg tattcaatgg
     1201 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     1261 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     1321 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     1381 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     1441 taaaattgcc tcgcttaagc acacggatga gagacagcct cctctcctcc gtgtgttact
     1501 ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta tgtcttccag
     1561 agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc aaaccgttct
     1621 ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg cgttttatgc
     1681 cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg cgcgtccggg
     1741 aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc ccgttgctgt
     1801 ccccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgact ttatccgcct
     1861 ggcggcgcta tggggcgtgg cgctaagaga gccggtgacc accgaagaac tggcctcatt
     1921 cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc aacaaaaact
     1981 ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac gagatgtgaa
     2041 tacggtcagc gaacctgaca gccaaattcc accaggattc agagggtaac gatgaaaaac
     2101 gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa acctgcattc
     2161 aaaacgggtg aagagctact ggcctggcag aaagaacaag gggcgatccg ctccgccgct
     2221 ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc cggtattcgt
     2281 ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg gcagatgaat
     2341 gcgttaagca aagcgcgcca gtatgtcgaa gagtttgacg gcaacatcgc cagcttcatc
     2401 ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg caacgagctg
     2461 ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc ggcgatgaaa
     2521 gataccttca ggaatagcgg taccagcgaa gaacaactgc ttaacgatct gagcaacgtt
     2581 gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga aaaagtgatc
     2641 atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat gctgaccaac
     2701 agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg tatgcgcctg
     2761 ggtaacagtt tgtgggtgat cttcaactgg gatagctacc gtagccgtgt aacaggtaaa
     2821 gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg agtaatgatg
     2881 atgaaaactg ttaaacatct tctgtgctgt gccattgccg ccagcgcatt aatttccacc
     2941 ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga acttggcaac
     3001 caaaacagca cgacacagga aggcggttgg tcgctcgcgt cattaactaa cttgcttagc
     3061 agcgggaacc aggccttaag cgcagataac atgaacaacg ccgcaggcat tctgcaatac
     3121 tgcgcgaagc aaaagctggc ttcggtaacc gatgccgaaa acatcaagaa ccaggtgctg
     3181 gaaaagctgg gcctgaacag tgaagagcaa aaagaagaca ccaactatct ggatggcatt
     3241 cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataacat cggaacgact
     3301 ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca ggggctgaac
     3361 ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac gcggctctct
     3421 cctgcccttt tccctgctcc tggacggttt tacccctgtt acacgggaag cgatcgcgat
     3481 caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga cactagaatt
     3541 gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa agcttaaacg ttggctaaac
     3601 ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc tttttctcgc
     3661 ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg cagccacatt
     3721 aacagtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg actattttac
     3781 tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga ccggtgctgg
     3841 cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacag cggtgttcgg
     3901 tgcgctgggc tggatcctgc gccgtcgtcg ccatcatccg caccattttg gttacagcct
     3961 gctggcgctc ttactggcgc tgggttcggt ggacgccagc ccggcatttc gtcagataac
     4021 ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt attataaaga
     4081 gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctatg gcgaaagtct
     4141 cgagcgaacc tattttgata acgaggcttt cccggatctc acgcctgaac tgggcgcgtt
     4201 gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccaggaacgg attacacgat
     4261 tgcgggcatg gtggcttctc agtgcggcat accgctgttt gccccctttg aaggcaacgc
     4321 ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa
     4381 ctcgggttat cagaactatt tcgtgcaggg cgcaaatctg cgttttgccg gtaaagatgt
     4441 gttcctgaaa tcgcacggct tcgaccactt atacggctca gaagagctga aaagcgtggt
     4501 agccgacccg cactatcgca acgactgggg attctacgac gataccgttc tcgatgaagc
     4561 gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt ttaccctgac
     4621 agtcgatacc catcacccgg atggttttat ttctcgtacc tgtaaccgca aaaaatatga
     4681 ttttgacggt aaaccgaatc agtcattcag cgcggtaagt tgcagccagg agaatatcgc
     4741 gacgtttatc aacaaaatca aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc
     4801 ttctgaccat ttagcgatga acaacacggc gtggaaatac ctcaataagc aggatcgcaa
     4861 taacctgttt tttgtcattc gtggcgacaa gccgcagcaa gagacgctgg cagtgaagcg
     4921 taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata actatctcgg
     4981 acttggtcgt agcagtttat ccgggcagtc gatgtcggaa atcttcctca atatcaaaga
     5041 gaaaacattg acgtggaagc cagatatcat ccgcctgtgg aaattcccta aagagatgaa
     5101 agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc atttccgttt
     5161 gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc
     5221 agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg tctgggttga
     5281 ccgttgctat aagatggcac aactctgggc tccggaactg gcactttcca ccgactggtg
     5341 tgtctcgcag gggcagcttg gcggtcagca aattgtccag catgttgaca aaacaacatg
     5401 gaagagcaag acggcattta aagatacggt catcgacatg gcgcgttaca aaggcaatgt
     5461 cgatacgctg aagattgttg ataacgatat tcgctacaaa gccgacagtt tcatctttaa
     5521 cgtcgccggt gcgccagaag aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg
     5581 gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca agcatccgct
     5641 gccgaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg ccagccgtcc
     5701 tattccggta cgcgtaggca atgaagaaca aactcttgtg ctgggcaatg aagtgaccac
     5761 caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg tgccgccgga
     5821 acctgtctca accaacgaag ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg
     5881 catggtggaa attaaagttg tggaacgtga agggtaattc ctgactgatg tgtaggtcgc
     5941 ataaggtgcg acctacacac cttctccggc ctacaaaaca tcattgagaa acagaaaaaa
     6001 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6061 aaagtaatta cgcctttaat taactcacga ttgttaatca catcgcgctc gtaggtttct
     6121 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cggcagtgat tttcccttcc
     6181 gccataagtc gaccgacttt agcgaaatct tccggcgtgg cgttgcggct gcccatcatc
     6241 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6301 acaaatacca ccgtgccacc gtgacgaatc agattcacgg tgttattcat cgcatgttga
     6361 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgcagctgc
     6421 gcgtcaaaat cttcggctga cgggtccagc acgggcaact ccagacgcgt tgccacatgt
     6481 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     6541 gccgccgcgc ccagaccgat tggccccgct ccgaccacca gcacctgctc gccgggagca
     6601 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgcggccgcc
     6661 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     6721 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     6781 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     6841 tttttaagat cagcaacatt tttacccagc ccaacaacct ccccacatat ttcatggcct
     6901 aaaacccgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     6961 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggaatctcg
     7021 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7081 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7141 cggaaaatct gttttgtgaa aacacgcgca taaaaatgtt ttaaatcggg ttttaatgga
     7201 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7261 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7321 cggaaatgta caacatcagc cgcactacag tgcgtcatat tctcagccac ttacgcgaat
     7381 gcggcgtctt aacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7441 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7501 ttttcacgat gataaaccag cgccagttac gcccagggga aactttttct gaactgcaac
     7561 tggctcgggc cgcgggtgtc agcccggtgg ttgtaagaga atatctttta aaattcggcc
     7621 gttataatct tattcaaagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     7681 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     7741 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     7801 acaggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     7861 attttcactc cttactgctt tcagctgccg acaatatctt ttttgaacaa tcgcttgaga
     7921 tcatatcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     7981 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8041 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8101 ccattaatga gaacatgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8161 cgggtgcacg gttttatcaa aagataaata ccggaaaatt cccgcttacc tatgctcaca
     8221 atcagtcgac taaaaatgtt tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc
     8281 cgttaatgtc aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg
     8341 agtccggcgt ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt
     8401 cgtcggcaga tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac
     8461 gcattcaaac caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc
     8521 gcagttcgct gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg
     8581 aaatcggcgc tttgctctcc gtgttttcac tcgcttacgg gattgcgcaa cttccttgcg
     8641 gcccactatt ggatcgtaaa ggcccgcgcc tgatgttggg actggggatg ttcttctggt
     8701 cactgttcca ggcaatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta
     8761 tcggtatggg aattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact
     8821 ggttcaacat caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg
     8881 gcgttgccgt aagcccaccg attctggcgg cgatgatgct ggtgatgggc tggcgcggga
     8941 tgtttattac cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc
     9001 gcaaccgcga gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg
     9061 tcaatgcccg ccgagatccg ctcagttttg ccgaatggcg cagcttgttc cgtaaccgca
     9121 caatgtgggg aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg
     9181 cctggcttcc tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga
     9241 tggcggctat ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg
     9301 actggctggt caaaggggga atggctccga ttaaaagccg taagatctgc attattgccg
     9361 ggatgttctg ttctgccgcc tttacgctgg tcgtaccgca agcgacaaca tccatgacag
     9421 cggttctgct gattggtatg gcactgttct gtattcactt tgccggaaca tcctgctggg
     9481 gcttgatcca cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact
     9541 ttgccagctt catctgcgcc tcttttgcgc cgatcattac tggttttatt gttgatacca
     9601 cccactcatt ccgtctggca ctaatcatct gcggttgcgt caccgcagcg ggggcactgg
     9661 cgtacatctt cctggttcgt cagccgatca acgacccacg gaaagattaa tctccctatg
     9721 cccgataaca ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctca
     9781 ccgctatctg ccacggccat tttacgcggc gcagctggca tcacggtttt taccacagcc
     9841 gatgtttcac gctgctgttg ctgctgttgc tgaatccgga acactgccac tgcttcggtc
     9901 aggcgactgg cctgctcttc cagcgcggcg gcggcggcgg cagattcttc caccagcgcg
     9961 gcgttctgtt gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca
    10021 cggctctgct catcagaagc agaagcaatt tcgcccataa tgtccgtcac gcgggtcaca
    10081 gcactgacaa tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc
    10141 actttcccca ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc
    10201 tgggccagat tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca
    10261 cgcgctgctt caaccgccgc gttcaaagcc agaatattgg tctggaaggc aatgccgtca
    10321 attacgctga taatatcggc gattttctgc gaactggtgg agatatcacg catagtctgc
    10381 accacgttat ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taacgccagg
    10441 tggctggcct ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg
    10501 ctggctgccg tctcttccag cgaagcagcc tgttgctcgg tgcgcgaaga gagatcgtta
    10561 ttgccggtag cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca
    10621 ccgacggtac gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc
    10681 tcattagagc catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg
    10741 ctgtcaatca ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg
    10801 aagatgaccg ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag
    10861 gaggcattgt tatcgctgac ggcgatatca tggagccgat cgttttgttc catgtaagcc
    10921 acatactgct tctcgaaacc gtcctgatat ccctgagtcg gctgatcaaa gaactcgttg
    10981 attttgcctg cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa
    11041 ttacgtttga tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg
    11101 taatccgccc agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca
    11161 gcaacggttg aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg
    11221 ttgagggtgt tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc
    11281 tggcgaatgg tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaagaac
    11341 agaccgcctg atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc
    11401 acaattttga tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg
    11461 cctggaaagg aaaaacttta tgaatatccc gcggagatta cgccgagtaa ttttattcac
    11521 actctgaatt taaaaagcca ttatttacat aaattattca caatataaat atgtgatatg
    11581 aatcacatat ttatcgtcac ttaaacgacg cctttgccgc tcaaccgcaa aactgaccgc
    11641 ttacatccct aaaataacca ctcagttatt taccttactt tacgcgcgcg taactctggc
    11701 aacatcacta caggatagcg gtcaatttac ctcctcaaac gcaacgcaaa cctagaacgg
    11761 cttcggccaa ctattaatca atacatgcca ggttttacta tggatactaa aaagatattc
    11821 aagcacatac cctgggtgat tctcggaatc atcggtgcat tctgcctcgc ggtagttgca
    11881 ttacgtcggg gggagcacgt cagcgccctg tggatcgtgg tcgcctctgt atcggtgtat
    11941 ctggtggcgt atcgctacta cagtctgtac atcgcccaga aggtgatgaa actcgacccc
    12001 acgcgcgcga cgcctgcggt tattaacaac gacggtctga actacgttcc gaccaaccgt
    12061 tacgtgttgt ttggtcacca cttcgccgct atcgccggtg ctggtccgct ggtgggtccg
    12121 gttctcgccg cgcagatggg ctacctgcct ggcacgctgt ggctgctggc gggggtcgtg
    12181 ctggccggtg cggttcagga ctttatggtg ctgtttatct cctctcgccg taatggcgca
    12241 tctcttggtg agatgatcaa agaagagatg ggaccagtac cggggactat cgcgctgttt
    12301 ggctgtttct taatcatgat catcatcctc gccgtcctgg cgctgattgt ggttaaagcc
    12361 ctggccgaaa gtccgtgggg tgtcttcacc gtttgctcaa ccgtaccgat tgcgctgttt
    12421 atgggtatct acatgcgctt tattcgtccg gggcgtgtgg gtgaagtctc tgtcattggt
    12481 atcgtgctgc tggttgcctc tatctacttc ggtggcgtga ttgctcacga tccgtactgg
    12541 ggtccggcac tgacctttaa agacaccacc attaccttcg cgctgattgg ctatgcgttt
    12601 gtttccgcac tgctgccggt gtggctgatc ctcgcaccgc gcgactatct ggcaaccttc
    12661 ctgaaaatcg gcgttatcgt cggcctggcg ctgggtatcg tggtgctgaa cccggaactg
    12721 aaaatgcctg ccatgaccca gtacattgac ggtactggcc cgctgtggaa aggcgctctg
    12781 ttcccgttcc tgttcatcac catcgcctgt ggtgcggtat ctggcttcca cgcgctgatc
    12841 tcttccggta cgacgccaaa actgctggct aatgaaaccg acgcgcgttt catcggctac
    12901 ggcgcaatgc tgatggagtc cttcgtggcg attatggcgc tggttgctgc gtccatcatc
    12961 gaaccgggtc tttacttcgc gatgaacacc ccgcctgctg gccttggcat caccatgcct
    13021 aacctgcatg aaatgggtgg cgagaacgcg ccgatcatca tggcgcagct gaaagacgtt
    13081 accgcacacg cggcagcgac cgtcagctcc tggggcttcg tgatttcgcc agagcagatc
    13141 ctgcaaaccg cgaaagacat tggtgagcct tctgtcctga accgtgcagg tggtgcgccg
    13201 acgctggcgg taggtatcgc tcacgtgttc cacaaagtgc tgccgatggc tgacatgggc
    13261 ttctggtatc acttcggtat tctgttcgaa gcactgttca tcctgaccgc gctggatgcg
    13321 ggtacccgtt ctggccgctt tatgctgcaa gacctgctgg gtaacttcat cccgttcctg
    13381 aaaaaaaccg actctctggt tgccggtatc atcggtactg cgggctgtgt gggtctgtgg
    13441 ggctacctgc tgtatcaggg cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg
    13501 ctgttcggta tttccaacca gatgctggca gccgtagcgc tggtactggg caccgttgtg
    13561 ctgattaaga tgaagcgcac ccaatacatc tgggtaactg ttgttccggc tgtatggctg
    13621 cttatctgca ccacctgggc gctgggcctg aaactgttca gcaccaaccc gcagatggaa
    13681 ggcttcttct acatggcaag ccagtacaaa gagaagattg ctaacggtac tgacctgacg
    13741 gcgcagcaga ttgccaacat gaaccacatc gttgtgaaca actacaccaa cgcgggcctg
    13801 agtattctgt tcctgattgt ggtgtacagc atcatcttct acggtttcaa aacgtggctt
    13861 gcggtgcgta acagcgacaa acgtactgac aaagaaacac cgtacgttcc aatcccggaa
    13921 ggcggcgtga agatctcttc gcaccactaa ccgtgtttag ccccgcttcg gcggggcttt
    13981 gttcttatca gagtgaacta tgtttggtaa cttaggacag gcaaaaaaat atctcggcca
    14041 ggcggcgaag atgttgattg gcattccaga ctacgacaac tacgtcgagc atatgaagac
    14101 caaccatccc gacaagccgt acatgagcta tgaagaattc ttccgcgaac gccagaatgc
    14161 gcgctacggc ggcgatggta aaggcggtat gcgctgttgt taatggagag agcatgaacc
    14221 cgattgcagt taccctactc accggttttt taggcgcagg caaaaccacc ctgctgcgcc
    14281 atattcttaa cgaacaacac ggctacaaga ttgccgtgat tgaaaacgaa ttcggcgaag
    14341 tctccgttga tgatcaactg attggcgacc gcgccacgca gatcaaaacg ctgaccaacg
    14401 gctgcatctg ctgttcgcgc tccaacgagc tggaagacgc actactcgac ctgctggata
    14461 atctcgacaa gggcaatatt cagttcgacc gtctggtcat tgaatgcacc ggcatggccg
    14521 atcccggccc gattattcag acctttttct cccatgagat tttatgccag cgttacctgc
    14581 tggacggcgt gattgcgctg gtggatgcgg tacatgccga tgagcagatg aaccagttca
    14641 ccatcgccca gtcacaagtg ggctacgccg accgtattct gctgactaaa accgacgtcg
    14701 caggcgaagc agaaaaactg cgtgaacgcc tggcgcgcat caacgcccgt gcaccggtct
    14761 acaccgtcac ccacggcgac atcgacctgg gcctgctgtt caacaccaac ggttttatgc
    14821 tggaagagaa cgtcatcagc accaaaccgc gtttccactt tatcgcggat aaacaaaacg
    14881 atatttcgtc gattgtggtg gaactggatt acccggtaga tatcagcgaa gtttcccggg
    14941 tgatggaaaa cctgctgctg gaatcggcgg ataaactgct gcgttacaaa gggatgctgt
    15001 ggattgacgg cgaacctaac cgcctgctgt tccagggcgt ccagcgcctc tacagcgccg
    15061 actgggaccg cccgtggggc gatgaaaaac cgcatagcac gatggtgttt atcgggattc
    15121 agttgccgga ggaagagatt cgggcggcgt ttgcggggtt gaggaagtaa ttatggatga
    15181 tggccgccat gtggcggcct gaattatttt ttgttctgat cgcgcagcat cgcttcacgt
    15241 aaaattgcgt tcaggcgagt ttgatagcct ttaccaggtc gcttcagcca ttccatcaca
    15301 tcagcatcaa tacgcaccga agcctgggtt ttcaatggcc gataaaactt accgcgttct
    15361 gcgttagacc attgctcatc gctggaaggc gggatatcac tgtaatcaat atcttcgtcg
    15421 aatttattcg ccagtgcctt cagttcggcc tcatgctgca catcgagtgt agagggtgtc
    15481 cctcgtttat gtttaaccat gctcatagcg gctcctctct ttacgatctg cttttcgtgc
    15541 actgatgatt cgaataattt catttccgct ttcgaaacgg atggtatgcg caaccagaat
    15601 aaccacgata ccgtgaacca ggccgatagt ctgccagcga tactcaccgt tttctatgcg
    15661 atcttgctgt gacaaatgtt gtggatcatc aaacactaac accgcatctt caaagcggat
    15721 gccatgtttc acccgattac ttttggcctt gtttgcatcc cattcaaact ccattggcat
    15781 agttgccatc cctaattgta tatacaaaaa tgtatctaca attattctgc aattatagcg
    15841 ttgatgttgg aaatagcatc acgttttgga taatcaacca tgaaattgac aatgagtaag
    15901 ttggacttga ttcatccaat agaatttctc gcctgacatg gcacactaaa cacctcgctg
    15961 tcgttaaggg attaccgtga aaaactataa agttattacc ccgctttttc ctacctatgc
    16021 acaggttaaa gctatgatga aagccgtctc aggctattcc ctgaaagcgg tacgcaatat
    16081 gattaccgcc attcgcgatc aaaccggtac gccgcaaaaa cccgttgact ggtcggaacc
    16141 tgacctgtgg atcagcgaac gcttaacggg cgaagatgcc gacatcgccc gtcgtatttg
    16201 ggatacagat aatcacatat tgaacccgcg ccacagctac ggctgctact tattcctcaa
    16261 ctatccccag tttgagctga tggaaagcac gccagatgat acctggcagc ccacctcgcg
    16321 cgggcaaaaa ttcctgcaag atgacgaaaa aacgctgcgt tcactggatg accaggaagg
    16381 cattctgcaa ctgcttgagc tgctggctgg ccgagaaatg tcgcgccgcg ccgatttatt
    16441 gccggaatgg caggcctttt tgcatcagca ttcgaaattc gcgtctgcaa gttcagtgaa
    16501 aagcacgctc tactcccgtc tctataacct tattgaccgt ggcatggtta ctcgcgaagg
    16561 tatgagctat tgcatcaccg ataccggacg cgcctggctt ggaaaagcgt tgccagcacg
    16621 aaaaagcgat ccgcgtaaag agctgctgga agcggtgaaa cgctacaacg agcagcaaaa
    16681 agagctgctg cgcgaacaaa tcagcacaat gaacccgtac aaatttgaac aactggtcgg
    16741 tcaattactg gaagcaatgg gctacgagca ggtcgaagtg accaaagcct caggcgataa
    16801 aggtgtggac gtgattggtc aggtgcaggt cggcatcacc actattaccg aagtggtgca
    16861 ggtcaaaaga atgcagaaca ccatcactcg accgtatatc gaccagttgc gcggagcgtt
    16921 gccttaccac aaggccattc gcggtacgct catcaccacc gggaaatttg cagcgaaatg
    16981 tgcagaagcg gcgctctttc cgggggccgc gcccattacg cttatcgacg gcgatcgctt
    17041 actggaactg ttaatcgaaa ataacgtcgg cattcgccgc agcaatgcag tggaattgct
    17101 ggatgtggat ttgcagttgt ttgatgagct ggagatggat taaagggcga aatccgccgc
    17161 ctcaaccatt acaatagcga cccaatttat ctgaacagag ttcatcatgg ccaaagcacc
    17221 aactaaaaaa gccaaagcaa aaaaaggctt tgaagaaaca ttatgggata ccgcaaacca
    17281 acttcgcggt agcgttgagt cctccgaata caagcacgtg gtgttaagcc tcgtgttcct
    17341 gaaattcatc agcgataagt ttgaagcacg ccgcaaaaaa atgattgccg atgggcaagc
    17401 tgacttcctt gagatggaag tgttctacca gcaggacaac attttctacc tgccggaaga
    17461 ggcgcgctgg tcgtttatca aacaaaatgc caaacaggac gatattgcgg ttcgtattga
    17521 caccgccctc tcgacaattg agaaacgtaa cccaaccctg aaaggcgcgc tgccagacaa
    17581 ctacttcagc cgtcagaatc tggaaaccaa aaaactggcg tcactgattg ataccatcga
    17641 caacatcgaa acgctggcac acgaaactga cgttgaaacg ttgtcgaaag aagacctggt
    17701 cggacgcgtt tacgaatact tcctcggtaa gtttgccgca accgaaggca aaggcggtgg
    17761 agagttctac acgcccaaat gtgtggtcac gctgttaact gaaatgctcg aacccttcca
    17821 gggcaaaatt tatgacccgt gctgcggctc ggcagggatg ttcgtgcagt cggtgaagtt
    17881 cgtcgagagc catcagggta aaagccgtga tatcgcgctg tatggtcagg aactgacagc
    17941 cacgacgtat aaactggcaa aaatgaacct cgccattcgc ggtctttcag ctaacctcgg
    18001 cgaacgcccg gcaaacactt tctttagcga ccagcacccg gacctgaaag ccgactacat
    18061 tctggcgaac ccgccgttca acctgaaaga ctggcgcaat gaagccgaat taaccgaaga
    18121 tccacgcttt gccggttatc gtatgccgcc aaccggtaac gccaactacg gctggatttt
    18181 gcatatgctc tccaagctgt cggctaacgg cacagcaggt tttgtgctgg caaacggttc
    18241 gatgagttct aacaccagcg gtgaaggcga gatccgtgca cagatgatcg aaaatgatct
    18301 gattgactgc atgatcgccc tacctggaca gttgttctac accacgcaga tcccggtgtg
    18361 tttatggttt atgaccaaat cgaaggctgc cgacccggcc aaaggttatc gtgatcgtca
    18421 gggcgagacg ctgtttattg atgcgcgtaa cctcggcacc atgattagcc gcacaactaa
    18481 agagttaacg gcggaagata ttgccacaat cgccgatacc taccatgcct ggcgcagcac
    18541 gccggaagaa ctggctgcac ggattgcgcg tggtgacagc aagctggaaa aatatgaaga
    18601 ccaggcaggc ttctgcaaag ttgcgaccct gcaagatatt aaagataacg actacgttct
    18661 gacaccgggc cgctatgtgg gtgcagccga gcaggaagaa gacggcgtgg catttgagac
    18721 caaaatgcgt gaattgtcga agacgttgtt tgagcagatg aagcaggcgg aagaactgga
    18781 tcgtgcgatt cgccagaatc tggaggcgct gggttatggg gagtaagtgg tgtatatatc
    18841 gcttaggtga tatagctgaa ttttcatacg gaaaaatgcc taaaaaggag tttgttggta
    18901 caggtgacta tgtcattttt tcaggatata aatacacaga aagctaccct gaaaaaaata
    18961 cttcggcagg tgagttaatt gttgttgccc
//
LOCUS       DACADF010000086        18721 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.087, whole genome shotgun sequence.
ACCESSION   DACADF010000086 DACADF010000000
VERSION     DACADF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.087"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            155..361
                     /locus_tag="ISB30_002954"
     CDS             155..361
                     /locus_tag="ISB30_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAP1990563.1"
                     /translation="MELYKEYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            462..971
                     /locus_tag="ISB30_002955"
     CDS             462..971
                     /locus_tag="ISB30_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAP1990564.1"
                     /translation="MNQQLFDDSTLIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQSLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            968..2386
                     /gene="phrB"
                     /locus_tag="ISB30_002956"
     CDS             968..2386
                     /gene="phrB"
                     /locus_tag="ISB30_002956"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAP1990565.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGAIDPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(2428..3909)
                     /gene="dtpD"
                     /locus_tag="ISB30_002957"
     CDS             complement(2428..3909)
                     /gene="dtpD"
                     /locus_tag="ISB30_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAP1990566.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            4180..4923
                     /gene="ybgI"
                     /locus_tag="ISB30_002958"
     CDS             4180..4923
                     /gene="ybgI"
                     /locus_tag="ISB30_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAP1990567.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKEMVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4946..5602
                     /gene="pxpB"
                     /locus_tag="ISB30_002959"
     CDS             4946..5602
                     /gene="pxpB"
                     /locus_tag="ISB30_002959"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAP1990568.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPACDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5596..6528
                     /gene="pxpC"
                     /locus_tag="ISB30_002960"
     CDS             5596..6528
                     /gene="pxpC"
                     /locus_tag="ISB30_002960"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAP1990569.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6518..7252
                     /gene="pxpA"
                     /locus_tag="ISB30_002961"
     CDS             6518..7252
                     /gene="pxpA"
                     /locus_tag="ISB30_002961"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAP1990570.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETIFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            7288..8079
                     /gene="nei"
                     /locus_tag="ISB30_002962"
     CDS             7288..8079
                     /gene="nei"
                     /locus_tag="ISB30_002962"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAP1990571.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAPQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(8076..9122)
                     /locus_tag="ISB30_002963"
     CDS             complement(8076..9122)
                     /locus_tag="ISB30_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP1990572.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMHG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIIAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(9302..10015)
                     /locus_tag="ISB30_002964"
     CDS             complement(9302..10015)
                     /locus_tag="ISB30_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP1990573.1"
                     /translation="MSESALLTSVTLSSQVKNHILNLIAEQKLTPGMPVPSEVQLIQK
                     LGVSRGIVREAYRSLAALGILETHSGKVPRIKKFDDEVLRVVFGFALATEQVTTEQIL
                     QVRRWLELGVVEAAARNGEEEDFANLRAEMAKIREQFNDVESFITHDIRFHQLLAVAS
                     KNPLFMMLLQALHQQLRQSIQEGLNAQSDQKEYGNHIITLHQKICDAVCARDVETATQ
                     AMKSHFDSPVSALINNSSS"
     gene            10279..10965
                     /locus_tag="ISB30_002965"
     CDS             10279..10965
                     /locus_tag="ISB30_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HAP1990574.1"
                     /translation="MWKSDNELFALMKTRLFTAVVGDILDTLGYMHQFVSPAIKPLDV
                     NTVVAGRAMPVLESDVYETKSTSGKTAIGEKPFGIMFEALDALKENDVYITTGSSLRY
                     ALWGGLMSTRAMHLKAAGAVLDGYARDSAEILELKFPTFCHGTYAQDQGPRGKVIDFG
                     VPIEFHGVRVNPGDIVFGDRDGLLIIPQVVEKEVITQALEKVATESEVRKAISDGMST
                     VQAFETFGVM"
     gene            10978..11991
                     /locus_tag="ISB30_002966"
     CDS             10978..11991
                     /locus_tag="ISB30_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP1990575.1"
                     /translation="MKALTIEQGSQARFVDIPEPVCGKGEVLINVERVGLCGSDLNTW
                     RGLNPLVNYPCIPGHEIGGTVLATGDMAGAIKPGDRVVVLPCTECGVCSSCLSGRPNA
                     CKNNQTLGVQRQGGMAQQLTMPAHKVIVCNALSVDELALVEPLAVGLHAARRAQINVG
                     EWVAVIGCGVIGLGAIAAASAMGARVIAIDIDDRKRNIALECGAQRFLNSQQCDVAAE
                     LVLLNDGRGPEVVIEAVGNQQTIEQATGLVAFSGRVVYVGYAKQPVSYQSAQFLMKEL
                     DIRGSRNATVADFRAVLALMESGRYPVSNLVSAHYPLEQADHAFSDWASDPGAYTKIL
                     IDL"
     gene            12003..12758
                     /locus_tag="ISB30_002967"
     CDS             12003..12758
                     /locus_tag="ISB30_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1990576.1"
                     /translation="MNAFSLHNKTALITGAASGLGLAMAKCMIVSGAKVIIADLNGEL
                     AQATAGTLGSQASWIQMDVGDTAAAQEKVDVLISEHGAIDILVNNAGNHCKKPIEEMS
                     VSDFESVLDVHVVGAFALTKALVPHMKQRGKGCVLFTASMTSFLGQPLVTGYSAAKSA
                     YLGMIRGMTTELAVHGIRVNGIAPGWIDTPMLRKAIEGDDERRNKILGRTPMKKFGKP
                     EDIGWAAVYLASDAAAFVSGQVLVVDGGALIGF"
     gene            12773..13084
                     /locus_tag="ISB30_002968"
     CDS             12773..13084
                     /locus_tag="ISB30_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HAP1990577.1"
                     /translation="MRKRALKLHAKDNCVVALNDIASGDIVWWDEGEMVACSSVTLGH
                     KLACEALNNGDSILKYGAIIGHATRDIAMGEHVHSHNLASNAIAIFHHDDAGYQSEEK
                     A"
     gene            13084..14280
                     /locus_tag="ISB30_002969"
     CDS             13084..14280
                     /locus_tag="ISB30_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HAP1990578.1"
                     /translation="MEGYVRQNGRPGIRNTVQVIFMVECASHVAKKIAEGFPCHDVQY
                     FGFSGCYPSDYGYRLVKNLATHSNVGGVLIISLGCENFDRRRLEQEVRESGRPCHTLV
                     IQENKGTTNTITLGKQLVAEMLEQLADTPRCILNWSDLVVGTICGGSDGTSGITGNPA
                     VGRAFDQLLEKGATCIFEESGELLGCEQHMMSRAASSQARDAIEVAMTKAERYYSLMG
                     LGSFSNGNAEGGLTTQEEKSLGAYTKSGDALIAGVILPTQQTPFPGLWLMDVVPDGEP
                     RFGYPNINDSSEALELIASGCHMVLFTTGRGSVIGSVIAPIIKVCNNPQTWEHLSDDM
                     DVNAGRVISEGATLDDIADDIMQVIEHVANGQYCAAERLEHQEFVLTYKAFDYSKSAV
                     CRADPR"
     gene            14308..15276
                     /locus_tag="ISB30_002970"
     CDS             14308..15276
                     /locus_tag="ISB30_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HAP1990579.1"
                     /translation="MKKLLTPLLFLLALFSPMLNAAPVTIKIAYESNPGEPADTVVKH
                     WAELVKKKSNGDIVFELYPSSQLGSKQDITEQALMGMNIITISDVGFLTDYDADLGIL
                     FGPYLTEDPQKLFAIYASDWFKQKEQKLREKGVHIVMSNYLYGTRQIIAKKPIRTPED
                     LKGMKIRVPNNVMQIKAIEAMGATPTPMPLGDVYPALTQGMIDGVENPVSVLYGQKLH
                     EQAKYLSMVNYLTNTSLWLGGEAFFSTLTPAQLDIIHQTAYEAGLYSQKITTEQDATM
                     LKTMQEQGVEIIYPDVAPFKEKALKVYQQFPEWTPGLYDTIQQQLK"
     gene            15336..15797
                     /locus_tag="ISB30_002971"
     CDS             15336..15797
                     /locus_tag="ISB30_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP1990580.1"
                     /translation="MRLLGKMIEVAAVLTLFILVIMTIAAVIMRYCFHMPLQWTEEVS
                     GLLMIWVVMFGAVVAERDNTHLSIPFLADALTSRGQRILAFLVSLMSIILLVVMAWSA
                     WKLAAGTAFKTTQILKISWFWIDIAVTVGAIGVACFTALQLIKKTPRKEGE"
     gene            15797..17107
                     /locus_tag="ISB30_002972"
     CDS             15797..17107
                     /locus_tag="ISB30_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP1990581.1"
                     /translation="MTALSELLAGSWLDNWLMIIPLMFVCLFINMPVWLAMFCGILTY
                     FLCYSNVPLMVAIQRLIATTQTPSLLAIPFFILLGTLMSYTGIAARILQIANVLIGRM
                     RGGLGGANILVSTMMGGVSASNLADAAMLSRMMVPEMEQKGYSRGFAAAITASGSLIT
                     PIIPPGIALILYGLVADVSIGSMFMAGILPGLLCSAMLIVTVWLVAIRRGLKPSSEHW
                     PKPKEVSVALVQAWPALFLIVAVIGGIRANIFTPTEAGAVAVLLVMFIGFVIYRELKI
                     SHVFTALSETARSTASVMLIIMASAALGWIFSLERAGITVAEFVTSLTDNKYVFLLII
                     NLLLLVLGMFLEGSAILLILVPLLKPVVAHFGIDPVHFGIIMILNLSLGTLSPPVGTV
                     MLLVCNTVQVSVGQFMREAVPLLISLILLLLVITYIPAISLLLV"
     gene            complement(17154..18437)
                     /gene="gltA"
                     /locus_tag="ISB30_002973"
     CDS             complement(17154..18437)
                     /gene="gltA"
                     /locus_tag="ISB30_002973"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAP1990582.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYAQRDFKSDIKK"
     gene            complement(18643..>18721)
                     /locus_tag="ISB30_002974"
     CDS             complement(18643..>18721)
                     /locus_tag="ISB30_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990583.1"
                     /translation="PKINLCNSVEVLTNSFGTVISGRQV"
BASE COUNT         4599 a         4402 c         5118 g         4602 t
ORIGIN      
        1 attacacggt gaatataaat aaaccatcaa taagcaaaaa taagtggtcg gatgagtagt
       61 aaaattacac aaacagcggt actattttca tcagataaac gaattcattt ttccggtgct
      121 attcaccggg tagatacaaa agggggagaa aagtatggaa ctctacaaag aatatcctgc
      181 atggcttatc tttttacgcc gtacttgtgc ggttgcagcg ggcgttctgg cgctgccttt
      241 catgcttttc tggaaagacc gcgcccgctt ttatagctac ctgcaccgcg tctggtcgaa
      301 aaccagcgat aaaccggtgt ggatggatca ggccgaaaag gcaaccggtg atttttactg
      361 attgccaaac atgacgttta aagaaaaccg ccggtggaaa cgctggcggt tttctttttt
      421 cggcgtctac acttactcct gtaaaccgtt caggagcagt aatgaatcaa caactttttg
      481 atgacagcac ccttatccgc atttttgctt tgcatgagtt acacaatctc aaagatcgag
      541 gactaacgcg cggggcgctt ctcgattatc acagccgcta taaactcgtc tttctggcgc
      601 attctcagcc ggagtaccgc aaacttggcc cgtttgtggc tgatattcac cggtggcaaa
      661 gtctggatga ctattacaac cagtaccgcc aacgcgtgat tgttttgctt tctcaccctg
      721 ccaaccggcg tgatcatact aacgttttga tgcacgttca gggatatttt cgcccacata
      781 ttgattccac agaacgccag cagctggcgg cgcttatcga cagttatcgc cgtggcgagc
      841 aatcgcttct tgcgccgctg atgcgtatca aacactatat ggcgctttat cctgacgcct
      901 ggctttcagg gcagcgttat ttcgaacttt ggccgcgtgt gattaacttg cgccatgcag
      961 gagttttatg actacccatc tggtctggtt tcgccaggat ttacgtctgc acgataatct
     1021 cgcactggct gccgcctgcc gcaattcgtc tgcacgcgtg ctggcgttgt atatcgctac
     1081 cccacgccag tgggcggcgc ataatgtgtc gccgcgtcag gctgaactta tcaatacaca
     1141 acttaatgcg ctgcaaaacg cgctggcgga aaaaggtatt cctttattgt tccgtgaagt
     1201 ggatgacttt gccgccagcg tcgaaatagt taaacaggtg tgcgcggaaa accgcgttac
     1261 tcatcttttt tataactatc agtatgaggt aaatgagcgg gcgcgggatg tggaagttga
     1321 aagagcgctg cgtaacgtgg tgtgtgaagg atttgatgac agcgtgatcc tgccgccggg
     1381 ggcggtgatg actggcaatc atgagatgta caaagtcttt acgcctttta agaatgcctg
     1441 gctgaaacgg ctgcgggaag ggatgccgga gtgcgtcgct gcgccaaaag tacgtagtag
     1501 tggagcgata gatcccgcgc catccattac gctgaattat cctcgtcagt ctttcgatac
     1561 tgcgcatttc ccggtggaag aaaaagcggc gattgcgcaa ttacgccagt tttgccagaa
     1621 cggtgccgga gagtatgagc aacaacgaga ttttccggca gtagaaggca ccagtcgttt
     1681 gtccgccagc ctggcaacgg gcgggttatc gcctcgccag tgtttgcatc gcttgttggc
     1741 ggaacagccg caggcgctgg acggtggagc cggtagtgtc tggcttagtg agctgatctg
     1801 gcgcgagttc taccgccatt tgatgacgta ttatccttcg ctgtgtaaac atcgtccgtt
     1861 tattgcctgg acggatcgtg tacagtggca aagtaatccc gcccatttaa aggcctggca
     1921 ggaaggcaaa acgggatacc cgattgtcga tgctgccatg cgtcagctta acagcactgg
     1981 ctggatgcat aaccggctac ggatgattac agccagtttt ctggtgaaag atttgttgat
     2041 cgactggcgc gaaggcgagc gatatttcat gtcgcagctg attgatggtg atttggcagc
     2101 taataacggt ggctggcagt gggccgcttc tacgggtact gatgcagcgc cgtattttcg
     2161 tattttcaac ccgacaactc agggcgagaa atttgaccgc gaaggcgaat ttatccgcca
     2221 gtggctaccg gagctgcgcg atgtaccggg gaaagcggtg catgagccgt ggaagtgggc
     2281 agagaaaaca ggtgtgacgt tggattatcc gcaaccgata gtcgatcaca aagaggcaag
     2341 gctgcgaacg ctggcagcgt acgaagaagc gaggaaagga gcctgataaa acgcagcgag
     2401 cgttttatca ggcatggtgc tggcttatta agactccagc gccagcgcgc ggtttctgaa
     2461 cttaagcgcc tgatacagcc aaatcatcag taccagtcct acacacgcca gtgcgcccca
     2521 ggtaatttga tcaaatactt caatatatgc attgatggag tagttgatcg ccccggaagc
     2581 atcaaacgaa gcctgcgatg tctgatcggc aatcacgccc gccagatagt tcgcaatcgc
     2641 gccggaaagc agcatataaa tgccggttaa tacgccggtc acgccgggga tttcaatgcg
     2701 cgtaatctgc gacatagcaa ccgggtcgat aaacagttcc gcaaagccca tcaccgccag
     2761 gcctaacacc atcagtggca gagaagagtg accatacatt gcggaccagc gggcgcttaa
     2821 ggtcagaatg cagaatccgg cgctcatcag gccaaggcca agagcaaatt ttccccagat
     2881 gcgcacggta cgattacctg cgacgctttc tttaaccacc cacgccagga acacaccgca
     2941 cagcataact gcgaaggcat taatcgactg gaacatagca gtcggaacgg tataaccaaa
     3001 catatcgcgg ttaacgaagc ggtcgatata aaggctaatc gagctacccc cctgttgtgc
     3061 gaatgcccag aacaacatgc tgaagaaggt gagggtaaca atcagcccca gctccttacg
     3121 ctgtttttgg ttttctgctt tgcgataaat ttttgccaga accccaaggc caatgatcgt
     3181 cgcgacaatc aaggcgtata ccgaccactc tttccagaac aacacggtaa tcagcgcagg
     3241 cgttgccacc agcagaacca gtagccatcc ccagttgggc aggagaaagt ttgtcgcacg
     3301 cagtaccttt ttgttaacgc cgcgggtatg tgtgaaatga cgattgccac ataagaaaat
     3361 gaccagaccc gcgatcatac ccaccgctgc caggccaaag cccatcgccc aactgtactc
     3421 ttcctgggcg aaaccacagg cgataggtgc gataatagac cccacgttac ccgccgcata
     3481 catcagcgag aaaccgccat cacgacgtgg atcggttggc tcatacagct caccgagcag
     3541 gcaactgacg ttggatttaa acagaccata gccgcagacg ataatcgcca gggacagata
     3601 gaggaatgac ggatggatct cactggcacc cagcaccaca tgaccgatcg ccatcaacaa
     3661 cgcccccagc atcaccgcca tgcgattgcc gagaacttta tccgccaaaa agccaccgag
     3721 gattggcgtg acatatacca gcgaacagta ggcgctaaat aactcgtagg cgtgattatc
     3781 gctatatttt agttgattgg tgagatagag gatcagcagg gcacgcatgc cgtaaaagct
     3841 aaaatattcc cagatttgca gcgcaacgac atagtaaata gcgcgcggtt gtgatgcgtg
     3901 tttattcata ataattccac aattgactgc aacgtcacgt aatagttgcg cggcgtgctt
     3961 gtgtttcctt aaagttaaaa ctttgataca gatctgatta tttatgcaat atgctgtctg
     4021 attgcataaa tatacattag ctgaagcgtg atgatataaa caatttgcgt cttttggcgt
     4081 cgggtttgtt tcttaatatg tcaaaagatt ggctatcgac gaacaggcga ggtaacgcta
     4141 tgttaacggt gtagctatca gaatacggaa ggcgcgaaga tgaaaaacac cgaactggaa
     4201 caactgatta acgaaaaact gaacagcgcg gcgattagtg attacgcgcc gaatggtttg
     4261 caggtggaag gcaaagagat ggtgcaaaaa attgttaccg gtgtaactgc cagccaggct
     4321 ttgctcgatg aggcagtgcg tctgggcgcg gatgcggtca ttgtgcatca tggctacttc
     4381 tggaaaggtg aatctccggt cattcgcgga atgaagcgta accgtttaaa aacgttgctg
     4441 gcgaatgata tcaacctgta tggctggcat ctgcccctgg acgcgcatcc ggagctgggt
     4501 aataacgcac agctggcggc gttactgggg atcacggtca tgggcgaaat tgagccgttg
     4561 gtgccgtggg gcgaactgac catgcctgtg ccgggactgg aactggcctc ctggattgaa
     4621 gcgcgtctgg gacgtaagcc gttatggtgt ggcgataccg gacctgaggt ggtccagcgc
     4681 gtcgcctggt gcacgggggg tgggcaaagt tttatcgata gcgccgcgcg ttttggcgtg
     4741 gatgctttta ttactggcga agtttctgaa cagaccattc attcagcccg cgagcaggga
     4801 ttgcattttt atgctgcggg tcaccatgcc actgaacgcg gtggtattcg cgcattgagc
     4861 gagtggctga atgaaaatac cgatcttgat gtgaccttta ttgatattcc taatcctgca
     4921 taacgaataa tcagagggat cgaaagtgca acgagcgcgt tgttatctga taggtgaaac
     4981 ggcggtagta ctggaactgg aaccgccggt gacgctggct agccagaaac ggatctggcg
     5041 actggcacag cgtctggtgg atatgccgaa tgtggttgaa gccattcctg gcatgaacaa
     5101 tatcacagtg attttgcgta atcctgagtc gctggcgctg gatgccatag agcgtttgca
     5161 acgctggtgg gaggagagtg aggcgctgga gccggagtct cgctttattg aaattccggt
     5221 agtttacggt ggcgcagccg gaccggattt ggcggtggtc gcggcgcatt gtggattgag
     5281 cgaaaaacag gttgttgaat tgcactcctc cgtggaatac gtggtctggt ttttaggttt
     5341 tcaaccgggc ttcccgtatc tcgggagttt gccggaacaa ctacacacgc cacggcgcgc
     5401 tgaaccgcgc ttgcacgttc cggcaggttc tgtcgggatc ggcgggccgc agaccggtgt
     5461 ttatccgctg gcaacgccgg gtggctggca gttgattggt catacctcac tcagcctgtt
     5521 tgatccggcg tgtgacgaac ccatcttatt acgtccggga gacagcgtgc gctttgtacc
     5581 gcagaaggag ggagtatgct gaagattatt cgtgcgggca tgtacaccac cgtgcaggat
     5641 ggcggccgtc acggctttcg tcagtctggt atcagccact gtggcgcact ggatatgcct
     5701 gcgttacgca ttgctaacct gctggtgggt aatgacgcca atgcccccgc gctggagatc
     5761 acgctcggtc agttaacggt tgagttcgaa actgatgggt ggtttgctct gacgggtgcc
     5821 ggttgcgaag cgcggctgga tgataatgcc gtctggaccg gctggcgatt gccgatgaaa
     5881 gcaggccagc gtttaacgct taaacgcccg cagcacggga tgcgcagtta tctggcggtc
     5941 gcgggtggta ttgatgttcc gccggtaatg ggatcatgca gcaccgatct caaagtgggg
     6001 attggcgggc tggaaggccg tttactgagg gatggtgacc gactcccgat tggcaaagcg
     6061 aagcgtgatt ttatggaagc gcagggcgtt aaacagctgc tctggggcaa ccgcattcgc
     6121 gccttgccgg ggccggaata tcatgagttc gatcgcgcct cgcaggatgc attctggcgt
     6181 tcgccctggc agcttagccc gcaaagtaac cgcatgggct atcgcttaca ggggcaaatt
     6241 ttaaaacgca ccaccgatcg cgaactgtta tctcacggtt tgttgccggg cgtggtgcag
     6301 gtgccgcata acggccagcc gattgtattg atgaacgacg cacagaccac cggtggttac
     6361 ccgcgtattg cctgtatcat tgaggctgat atgtaccatc tggcgcaaat tccgctcggt
     6421 cagccgattc attttgtcca gtgttcactg gaagaggcac tgaaagcgcg gcaagatcag
     6481 caacgttatt tcgaacaatt agcgtggcgg ctgcacaatg aaaattgacc tgaacgccga
     6541 tctgggcgaa ggctgcgcca gcgacgcaga gctattaacg ctggtttcct ctgccaatat
     6601 tgcctgtgga tttcatgcag gagatgcgca aaccatgcag gcttgcgtgc gtgaagcaat
     6661 aaaaaatggt gtcgcgattg gcgctcaccc gagttttccc gacagggaaa attttggtcg
     6721 cagcgccatg cagctgccgc cagaaaccat tttcgcccag acgctgtatc aaattggcgc
     6781 gctggcagcg attacccgtg cgcaaggcgg cgtaatgtgt catgtcaaac cgcacggcat
     6841 gttatacaac caggcggcga aagaggcaca actggcagac gctatcgcca gagcggtata
     6901 cgcttgcgat ccggcattga ttctcgtcgg gctggcagga agcgagctga ttcgtgcagg
     6961 cgaacgatat ggcctggtaa cgcgcgagga agtgtttgcc gatcgtggtt atcaggcaga
     7021 cggttcgctg gtgccgcgaa gccagccagg cgcgttgatt gaaaacgaag aacaggcgct
     7081 ggcgcaaacg ttggagatgg tgcaacacgg cagagtcaaa agtatcaccg gcgaatgggc
     7141 aatggtcacg gcgcaaacgg tctgcctaca tggcgatggc gagcacgcac tggcgttcgc
     7201 ccgccgatta cgagctacat ttgctgaaaa ggggattgtt gtcgcagcat aaccccgctt
     7261 aataaagaat gaaaaaagga tatcaccatg cctgaaggcc cggagatccg ccgtgcagcg
     7321 gataacctgg aggcggcgat caaaggcaaa ccactaactg atgtctggtt tgccttcccg
     7381 cagttaaaat cttatcaatc acgacttatc ggtcaacacg ttacccatgt ggaaacgcgt
     7441 ggtaaggcgt tgttaactca tttttccaac gacttaacgc tctacagcca taatcagctt
     7501 tacggcgtct ggcgcgtggt tgataccggc gaagagccgc agaccacgcg agtattgcgg
     7561 gtaaaactgc aaacggctga caaaaccatt ctgctttata gcgcctcgga tattgagatg
     7621 ttgactccgg aacaactgac cacgcatccg tttttacaac gcgttggtcc cgatgtgctg
     7681 gatccgaatc tgacgccgga ggtggtaaag gaacgattat tgtcgccgcg ctttcgtaac
     7741 cgtcagtttg ctggattact gctcgatcag gcgtttctgg caggacttgg caattatttg
     7801 cgggtggaga tcctctggca ggttgggttg accggaaatc ataaagcgaa agatctcaat
     7861 gcgccgcaac tggatgcact cgcacacgcg ttactggata ttccgcgact ttcttacgct
     7921 acgcgggggc aggtggatga aaacaaatat catggggcgc tgtttcgctt taaggttttt
     7981 catcgtgatg gcgaaccttg cgaacgctgt ggcggcatca ttgagaaaac cacgctgtca
     8041 tcgcgcccgt tttactggtg cccaggctgc cagcactagg ccgaacgctt cggcgcatag
     8101 gttgaaataa accgtgcgat ggcaggcccc gtcagcaaaa tactgaacag gcgcagggtt
     8161 tgcatcgcaa tgatgagcgc catatcggca ttgctccctg cggcgatgac cgccacggta
     8221 tcaagcccgc cggggctggt ggcgaggtag gcggtcataa aatcaatatg cataaaccgg
     8281 gtcagccccc acgccatgcc cgcacaaata gccagcagag caaaaatcga cagcaggatt
     8341 tgcggtagcg ggcgcaatgc ccgcagtaat atttgcttat cgaaaccaag accaatccgc
     8401 cagccaattg ccatatatgc catcgccagc agccattccg ggagttcaat cgtgatgagc
     8461 tgaccagact ggagcactgc gcccgccagc atcgggatga gcatcgtgcc ggaaggaaga
     8521 cgtagcaagc atcccgccgt accggcaacg accgcgagca aaatcgtaag caggagatta
     8581 atgctcactg gcgggaacca gacgatctgc tggttaaccg cttcagcgtt atcgcccagc
     8641 atcatgcggg tgaccagaac agcggctcct gcgacgaaca gcactcgcag atattgcata
     8701 aacgccacca ggcgaatatc tgcgccgtaa tcttgcgcca ttgcgaccat tgccgccgcg
     8761 ccgccaggag aagaacccca ggccccggta ttcccaggca gtgagctata gcgcaccaat
     8821 aaccaaccca cgatggcgct ggaaagcagc gtcaccagta aaatcgcaag cacgatcggc
     8881 cagttgacgg ccagcgtggt gagaatcgaa ccagtgaggt tttgtgcaat catgcagcca
     8941 agaatggctt gggcagcgag aaaagcggag cggggaagtt gcagagtgac tccgtgcata
     9001 ctgaagatga tccccgcgat catcgggcca agcaggagtg ccgccgggag atgtaaagcg
     9061 aggaagccaa tagaaaggag aagtgacaga acacataaca ttccccactg tagcactggc
     9121 atccgtcact ccctgtttaa cttacctttg gtgtatcgat acagcataaa gaatgcgcct
     9181 caacgaaaaa atagcggtaa tcaagaaaaa gacagatttt ggctaaaggg cgtattaagt
     9241 accgttgtct ccctctcgtt tgtaaaagag ggaagagtaa acgattgaac tgagataagt
     9301 cttatgacga ggaattattg atcagtgcac tgaccgggct gtcgaagtgt gatttcatgg
     9361 cttgagtcgc cgtttcgaca tccctggcac acacagcatc gcagattttc tggtgtagtg
     9421 tgatgatgtg attgccatac tctttctgat cgctttgggc gtttaaaccc tcctggatgg
     9481 attgccgcaa ctgctgatgt aatgcctgca ataacatcat aaaaagcgga ttttttgagg
     9541 caacggcaag taactgatgg aagcggatat catgggtgat aaagctttca acatcattga
     9601 actgttcgcg gatttttgcc atttcagccc gtaggttagc aaaatcttcc tcttcgccat
     9661 tacgggcagc agcttccaca acgcccaact ccagccagcg gcgtacttga agaatttgct
     9721 ctgttgtcac ttgttcggtc gccagggcaa atccaaacac cacgcgcagc acttcatcat
     9781 caaatttttt gattcgtggc actttaccgc tgtgcgtttc cagaatcccc agggcggcaa
     9841 gtgaacgata ggcctcacgc acaatacccc gactgacacc caatttctga atcagctgaa
     9901 cttcactggg aactggcatc cccggtgtca atttttgttc ggcgatcaga ttaagaatat
     9961 gatttttgac ctgtgacgat aacgttacgc tggtcaataa cgcactctca ctcattttta
    10021 ctcctgttgt actttttcct ggtcggtgaa atccagaata ccgggactgt atccttcata
    10081 ttttcctcca gaacccttga ttatcaatag actactgcgc ctgtgcgccc gccatgcagt
    10141 gcgaatcaaa gacatattgt gtgaaagagt ggcgaagaaa tagtaaatac acaacttttt
    10201 cgtgacgccc ttcgcagtat tgtgcgcttt ttctccttac cctgtaaacc tgttaaacag
    10261 gcttacagga cgaaaataat gtggaagagt gataacgaac tttttgcttt gatgaaaacc
    10321 cggctattta ccgccgtggt tggcgatatt ctcgacacgc tgggctatat gcaccagttt
    10381 gtttctccgg caatcaagcc gctggatgtg aatacggtgg tggcaggccg ggcaatgccg
    10441 gtgctggagt cggacgttta cgaaaccaaa tcgacgtcgg gtaagacggc gattggcgaa
    10501 aaaccgtttg gcatcatgtt tgaagcgctg gatgccctca aagagaacga tgtttacatc
    10561 actacgggca gctctctgcg ctatgccttg tggggcgggt tgatgtctac ccgtgccatg
    10621 catctgaaag cagcaggtgc ggtactcgat ggttacgccc gtgactctgc tgaaattctt
    10681 gaattgaaat ttcccacttt ttgtcatggc acttacgcac aggatcaagg tccgcgcggc
    10741 aaggtcatcg acttcggcgt acctattgaa tttcacggcg tgcgagtgaa tccgggcgat
    10801 atcgtgtttg gtgatcgtga cggattactg attattcccc aggttgttga gaaagaagtt
    10861 attacccagg ccctggaaaa agtggcaact gaaagtgaag tgcgcaaagc catcagtgat
    10921 ggcatgagca cggtacaggc ctttgaaacc ttcggcgtaa tgtaaggaga gcaatgaatg
    10981 aaggcattaa ccattgagca gggaagtcag gcgcgatttg ttgatatccc cgaacctgtc
    11041 tgcggtaaag gagaagtgct aatcaatgtt gagcgtgtcg gactgtgtgg ttctgacctc
    11101 aatacctggc ggggactaaa tccgctggtt aattatccct gtattccggg gcatgaaatt
    11161 ggcggcacgg tgctggcaac tggcgatatg gcaggggcga tcaagccagg tgaccgggtg
    11221 gtcgttctgc cttgcactga gtgtggcgtg tgcagctcct gtctgagcgg tcgtcctaac
    11281 gcctgtaaaa acaaccaaac tcttggtgtg caacgtcagg gggggatggc gcaacaactg
    11341 acgatgcctg cacataaagt cattgtctgt aatgccttaa gtgttgatga gttagcgctg
    11401 gtagaaccgc tggccgtggg actacatgcg gccagacgtg cgcaaattaa tgtgggtgaa
    11461 tgggtcgcag ttatcggttg tggggtaatt ggccttggcg ctatcgccgc ggcatccgcc
    11521 atgggggcgc gcgtaattgc tatcgatatt gatgatcgca agcggaacat tgccctggaa
    11581 tgcggtgcgc aacgttttct taattcgcag caatgtgacg tggcggcaga actggtgctg
    11641 ttaaatgacg gtcgcggccc ggaagtggtg attgaagcgg tgggtaatca acaaaccatt
    11701 gaacaggcta cagggttggt ggcgtttagc ggtcgtgtgg tttatgtcgg ttacgccaaa
    11761 cagccagtga gttatcagag tgcgcagttc ctgatgaaag aactggatat ccgtggttcg
    11821 cgcaatgcca ccgtggcgga ttttcgtgcc gtgctggcat tgatggagtc cgggcgttat
    11881 ccggtcagca atctggtgag tgctcactac ccgctggaac aggctgatca cgcttttagc
    11941 gactgggcaa gtgatcccgg cgcgtatacc aaaatcctca ttgaccttta aggagcgaca
    12001 ccatgaatgc tttttcttta cataacaaaa ccgcgttgat caccggtgca gccagtgggc
    12061 tggggctggc aatggcgaaa tgcatgatag tcagcggggc gaaggtcatt attgctgacc
    12121 tgaacggcga gctggcgcaa gcgaccgccg gaacattggg gtcgcaggcc agttggatac
    12181 aaatggatgt cggggatacc gcagccgcgc aggaaaaagt tgatgtgctt attagcgaac
    12241 acggggctat cgatatcctg gtcaataacg caggcaatca ctgcaaaaaa ccgattgaag
    12301 agatgagcgt cagtgatttt gagtcagtac tcgacgttca cgtagtcggc gcatttgcgt
    12361 taaccaaagc gctggtgccg catatgaaac aacgcggtaa aggctgtgtg ctttttactg
    12421 catcgatgac ctcatttctt gggcagcctc tggttactgg ctacagcgca gcgaagtcag
    12481 cctatctcgg catgattcgc ggtatgacca cggagcttgc cgttcatggg attcgcgtca
    12541 atggtattgc gccaggctgg atcgatacac caatgttgcg taaggccatt gaaggtgatg
    12601 acgaacgccg taacaaaatc ctgggtcgta ccccgatgaa aaaattcggc aagccggagg
    12661 atatcggttg ggctgcggtt tatctggctt cagacgcagc ggcgtttgtc agtggtcagg
    12721 tgctggtggt ggatggcggc gcattgatcg gcttctgatg tacggaggaa ttatgcgcaa
    12781 acgtgcgtta aagctacatg ccaaagataa ctgtgtagtc gcgctgaatg acatcgccag
    12841 tggcgatatt gtctggtggg atgaggggga aatggtggcc tgtagttccg ttaccctggg
    12901 gcacaaactg gcctgcgaag cgctgaacaa tggtgattcg attctgaaat acggcgccat
    12961 tatcggacac gccactcgcg atattgccat gggtgaacat gtgcattccc ataatcttgc
    13021 cagcaatgcc atcgccattt ttcatcatga tgatgccggc tatcagtcag aggaaaaagc
    13081 ataatggaag gttatgtacg gcaaaatggt cgaccaggta ttcgtaatac tgtgcaggta
    13141 atctttatgg tggagtgtgc cagccatgtc gctaaaaaga ttgccgaagg atttccttgt
    13201 cacgacgtgc agtattttgg tttttcaggt tgttatccca gcgattatgg ctatcggctg
    13261 gtgaaaaatc tggcgactca tagcaacgta ggtggtgtac tgattatttc gctggggtgc
    13321 gagaatttcg atcgccgtcg gctggagcaa gaagtacgag aaagtgggcg accgtgccat
    13381 acgctggtca ttcaggaaaa taaaggcacg accaacacca ttactctggg taagcagttg
    13441 gtcgctgaaa tgctggaaca gcttgcggat acaccgcgtt gtatcctgaa ctggagcgac
    13501 ctggtggttg gtactatctg cggtggctcc gatggcacca gtggtatcac gggtaacccg
    13561 gcagtaggac gtgcctttga ccaactgctg gagaaagggg ctacctgtat ttttgaagaa
    13621 agcggtgagt tgcttggttg tgagcagcat atgatgtcgc gcgcggctag ttcgcaggca
    13681 cgtgatgcca tcgaagtggc aatgacaaaa gccgaacgtt attacagttt gatggggctg
    13741 gggagttttt ctaacggtaa tgctgaaggc ggcttaacca ctcaggaaga aaaatccctt
    13801 ggggcttata ccaagtcggg cgatgcgctg attgctggcg ttattttgcc gacgcaacaa
    13861 acgccctttc ctgggctttg gttgatggac gtggtgcctg atggtgaacc tcgttttggg
    13921 tatccgaata ttaacgattc ctcagaagca ctggaactta tcgcctctgg ctgtcatatg
    13981 gtgttattta ccacaggtcg cggttcagtt attggttcag tgatcgctcc tattattaaa
    14041 gtgtgtaata acccgcagac gtgggagcat ttatctgacg atatggacgt taatgccggt
    14101 cgtgtaatta gcgaaggcgc aacgctcgac gatattgcag acgatattat gcaggtgatt
    14161 gaacatgtcg ccaacggtca atattgtgcc gcagaacgac tggaacatca ggagtttgtt
    14221 ttaacgtata aagcatttga ttacagcaaa agtgcagtct gtcgggcaga tccccgttaa
    14281 tcgataaagc tcttacaggt aataataatg aaaaaattac tgacaccact tttatttctt
    14341 ttagctttat ttagtccgat gctgaatgcc gcaccggtaa ctatcaaaat cgcctatgaa
    14401 agtaaccccg gcgagccagc ggatacagta gtgaagcact gggcagaact tgtgaagaaa
    14461 aaaagcaatg gagatattgt tttcgaactt tacccaagtt cacagttagg ttctaaacaa
    14521 gatattactg aacaagcgtt gatggggatg aatattatca ccatcagtga tgtaggattt
    14581 ttaactgact acgatgcaga cttgggaata ttgtttgggc cttatttaac cgaagatccg
    14641 cagaaactgt ttgcgattta tgccagtgac tggtttaaac aaaaagaaca gaaactacgg
    14701 gaaaaaggtg tgcatattgt catgagcaac tatctgtacg gtaccaggca gatcattgct
    14761 aaaaaaccga ttcgtacacc tgaagacttg aaagggatga agatccgcgt gccgaataac
    14821 gtcatgcaga ttaaagctat cgaagcgatg ggcgcaaccc caacaccaat gccgctggga
    14881 gatgtttatc cggcgctgac tcaggggatg atcgatggtg tggaaaaccc ggtttctgtt
    14941 ctctatgggc aaaagctgca tgagcaggcg aaatatctgt caatggtgaa ctatctgacc
    15001 aatacatcat tatggttggg tggcgaagcg tttttcagta ccctgacacc tgcgcagtta
    15061 gatattattc accagacagc gtatgaagct ggtttatata gccagaaaat taccactgag
    15121 caggatgcga caatgttgaa aaccatgcag gagcagggcg tggaaatcat ctatcctgac
    15181 gtcgcccctt tcaaagaaaa agccttaaaa gtctatcaac aattcccgga atggacccct
    15241 ggtttgtatg acactattca gcaacagctg aaataacttc atggcataag gactggtcgg
    15301 tgtttcggca ccgaccgtta agagcagagg tgtttatgcg tcttttaggg aaaatgattg
    15361 aagttgctgc ggtattaacg ctctttatat tggtaatcat gaccattgct gcggtaataa
    15421 tgcgttactg ttttcatatg ccattacagt ggacagaaga ggtttccggt ttattaatga
    15481 tttgggtagt catgtttggt gcggttgttg ctgaacgaga taatacccat ttatcgattc
    15541 catttttagc cgatgcactt acttcacgcg ggcaacgtat attagcgttt ttagtctctc
    15601 tgatgtctat tattttgctg gtggtcatgg cctggtcagc atggaaatta gctgccggga
    15661 cagcctttaa aaccacgcaa atattaaaaa tatcctggtt ttggatcgat attgccgtca
    15721 cggttggcgc tatcggtgtc gcctgtttta ctgccttaca actgatcaag aaaacgccac
    15781 gcaaagaagg tgaataatga cagcattgag tgaattactg gcgggaagct ggctggataa
    15841 ctggctgatg atcattccgc ttatgttcgt ctgtttattt atcaatatgc cggtatggct
    15901 ggcgatgttt tgcggcattc tgacctactt cctttgctat tcaaatgtgc cgttaatggt
    15961 ggcaattcag cgtttgattg ccacgacgca aaccccttcg ctgttagcta ttccgttttt
    16021 tatcttatta ggcacgttaa tgagctatac cggtattgcc gcgcgtattt tgcaaattgc
    16081 caacgtgttg attggcagaa tgcgcggtgg cctgggtggg gctaatattc tggtcagtac
    16141 catgatgggc ggtgtcagtg cctcaaatct ggcggatgcc gcaatgttat cgcgcatgat
    16201 ggtgccggaa atggaacaga aaggttattc ccgtggcttt gcggcggcga taacggcaag
    16261 tggttcgctg attacaccaa ttattccacc gggtattgca ctgatccttt acggactggt
    16321 ggcggatgtc tccatcggct ccatgtttat ggccgggatc ctgccaggtc tgctgtgctc
    16381 cgccatgttg atcgttaccg tatggttggt ggctattcgt cgcggtttaa aaccttccag
    16441 cgaacactgg ccaaaaccga aagaagtgtc agtggcgctg gtgcaggcct ggccagcact
    16501 gttcttaatt gttgcggtta tcggtggtat tcgggcaaat atctttacgc caacggaagc
    16561 cggagcggta gcagtattat tggtaatgtt tattggtttt gtaatttatc gcgaactgaa
    16621 aatcagccat gtatttacag cactctctga gacggcgcgt tccacggcat cggtcatgtt
    16681 aatcattatg gcgagtgccg cgctgggctg gatattttcc cttgagcggg caggtatcac
    16741 ggttgcggag tttgtaactt ctctgaccga taataaatac gtctttttat tgattatcaa
    16801 tctattgctg ctggtgcttg gcatgttcct tgaaggtagt gccattctgc tgatattagt
    16861 cccattactt aaacccgtgg tggctcattt tggcattgat ccggtgcatt ttggcatcat
    16921 tatgatcctc aacttatcgt taggaacgtt atcaccacct gtcggaacgg tgatgctgct
    16981 ggtatgtaat accgtacagg tttctgtcgg gcaatttatg cgtgaagcgg tgccattact
    17041 gatcagtctt attttacttc tgttggtcat tacctatatt ccggcaatat ctttgctgct
    17101 ggtgtagaat taaaaaccct gcgtgtccac gcagggtttt gtcggtgtca tttttacttc
    17161 ttaatatccg acttaaaatc acgctgcgcg tagccggtat acagctgacg cggacgggca
    17221 atcttcatac cgtcactgtg catttcgctc cagtgggcga tccagccaac cgtacgtgcc
    17281 atagcgaaga tcacggtgaa catagaagac ggaataccca tcgctttcag gatgatacca
    17341 gagtagaaat cgacgttcgg gtacagtttt ttctcgataa agtacgggtc gttcagcgcg
    17401 atgttttcca gctccatagc cacttccagc aggtcatctt tggtgcccag ctctttcagc
    17461 acttcatggc aggtttcacg cattacggtg gcgcgcgggt cgtagttttt gtacacgcga
    17521 tgaccgaagc ccatcaggcg gaaagaatca tttttgtctt tcgcacgacg aacaaattcc
    17581 ggaatgtgtt taacggagct gatttcttcc agcattttca gcgccgcttc gttagcacca
    17641 ccgtgcgcag gtccccacag tgaagcaata cctgctgcga tacaggcaaa cgggttcgca
    17701 cccgaagagc cagcggtacg cacggtggag gtggaggcgt tctgttcatg gtcagcgtgc
    17761 aggatcagaa tacggtccat agcacgttcc agaatcggat taacttcata cggttcgcac
    17821 ggtgtggaga acatcatatt caggaagtta ccggcgtagg agagatcgtt gcgcgggtaa
    17881 acaaatggct gaccaatgga atacttgtaa cacatcgcgg ccatggtcgg catttttgac
    17941 agcaggcgga acgcggcaat ttcacgatga cgaggattgt taacatccag cgagtcgtga
    18001 tagaacgctg ccagcgcgcc ggtaatacca cacatgactg ccattggatg cgagtcgcga
    18061 cggaaagcat ggaacagacg ggtaatctgc tcgtggatca tggtatgacg ggtcaccgta
    18121 gttttaaatt cgtcatactg ttcctgagtc ggtttttcac cattcagcag gatgtaacaa
    18181 acttccaggt agttagaatc ggtcgccagc tgatcgatcg ggaaaccgcg gtgcagcaaa
    18241 ataccttcat caccatcaat aaaagtaatt ttagattcgc aggatgcggt tgaagtgaag
    18301 cctgggtcaa aggtgaacac accttttgaa ccgagagtac ggatatcaat aacatcttga
    18361 cccagcgtgc ctttcagcac atccagttca acagctgtgt ccccgttgag ggtgagtttt
    18421 gcttttgtat cagccattta aggtctcctt agcgccttat tgcgtaagac tgccggaact
    18481 taaatttgcc ttcgcacatc aacctggctt tacccgtttg ttatttggct cgccgctctg
    18541 tgaaagaggg gaaaacctgg gtacagagct ctgggcgctt gcaggtaaag gatctattga
    18601 tgacgaataa atggcgaatc aagtacttag caatccgaat tattaaactt gtctaccact
    18661 aataactgtc ccgaatgaat tggtcaatac ttccacactg ttacataagt taatcttagg
    18721 t
//
LOCUS       DACADF010000087        18500 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.088, whole genome shotgun sequence.
ACCESSION   DACADF010000087 DACADF010000000
VERSION     DACADF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.088"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            25..1164
                     /gene="arnB"
                     /locus_tag="ISB30_002975"
     CDS             25..1164
                     /gene="arnB"
                     /locus_tag="ISB30_002975"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAP1990584.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALATLPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1168..2136
                     /gene="arnC"
                     /locus_tag="ISB30_002976"
     CDS             1168..2136
                     /gene="arnC"
                     /locus_tag="ISB30_002976"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAP1990585.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHVLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2136..4118
                     /gene="arnA"
                     /locus_tag="ISB30_002977"
     CDS             2136..4118
                     /gene="arnA"
                     /locus_tag="ISB30_002977"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAP1990586.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPRGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKALPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4115..5005
                     /locus_tag="ISB30_002978"
     CDS             4115..5005
                     /locus_tag="ISB30_002978"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAP1990587.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQMAGSH"
     gene            5005..6657
                     /gene="arnT"
                     /locus_tag="ISB30_002979"
     CDS             5005..6657
                     /gene="arnT"
                     /locus_tag="ISB30_002979"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAP1990588.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALMTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQGRLVLIQYRPK"
     gene            6654..6989
                     /gene="arnE"
                     /locus_tag="ISB30_002980"
     CDS             6654..6989
                     /gene="arnE"
                     /locus_tag="ISB30_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAP1990589.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGVAFIIG
                     GIVILGSTV"
     gene            6989..7375
                     /gene="arnF"
                     /locus_tag="ISB30_002981"
     CDS             6989..7375
                     /gene="arnF"
                     /locus_tag="ISB30_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAP1990590.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7369..7635)
                     /gene="pmrD"
                     /locus_tag="ISB30_002982"
     CDS             complement(7369..7635)
                     /gene="pmrD"
                     /locus_tag="ISB30_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAP1990591.1"
                     /translation="MEWLVKKSCCNKQNNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(7745..9100)
                     /gene="menE"
                     /locus_tag="ISB30_002983"
     CDS             complement(7745..9100)
                     /gene="menE"
                     /locus_tag="ISB30_002983"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAP1990592.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AAQGVVAGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALTSLHIQQVEGVHAVAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(9097..10059)
                     /gene="menC"
                     /locus_tag="ISB30_002984"
     CDS             complement(9097..10059)
                     /gene="menC"
                     /locus_tag="ISB30_002984"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAP1990593.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEDAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVKAAHALGLTAVISSSIESSLGLMQLAR
                     IAAWLTPDTIPGLDTLDLMQVQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(10059..10916)
                     /gene="menB"
                     /locus_tag="ISB30_002985"
     CDS             complement(10059..10916)
                     /gene="menB"
                     /locus_tag="ISB30_002985"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAP1990594.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10931..11689)
                     /gene="menH"
                     /locus_tag="ISB30_002986"
     CDS             complement(10931..11689)
                     /gene="menH"
                     /locus_tag="ISB30_002986"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAP1990595.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTDLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11686..13356)
                     /gene="menD"
                     /locus_tag="ISB30_002987"
     CDS             complement(11686..13356)
                     /gene="menD"
                     /locus_tag="ISB30_002987"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAP1990596.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERGRFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETTLVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13445..14740)
                     /gene="menF"
                     /locus_tag="ISB30_002988"
     CDS             complement(13445..14740)
                     /gene="menF"
                     /locus_tag="ISB30_002988"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAP1990597.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGYLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTLLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTRTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14819..15124)
                     /gene="elaB"
                     /locus_tag="ISB30_002989"
     CDS             complement(14819..15124)
                     /gene="elaB"
                     /locus_tag="ISB30_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAP1990598.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15179..15640)
                     /locus_tag="ISB30_002990"
     CDS             complement(15179..15640)
                     /locus_tag="ISB30_002990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP1990599.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            15705..16622
                     /gene="rbn"
                     /locus_tag="ISB30_002991"
     CDS             15705..16622
                     /gene="rbn"
                     /locus_tag="ISB30_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAP1990600.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEISTGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFSI"
     gene            complement(16743..18485)
                     /locus_tag="ISB30_002992"
     CDS             complement(16743..18485)
                     /locus_tag="ISB30_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP1990601.1"
                     /translation="MRNKNIIMLLISSLILSGCGPEPEDKKSQQQQSTTPTDQQVLAA
                     QQAATKVAEQSAAKAAADAKALAQQEVQQYSDKQALLGRLQAAPKYQHAAREKAASQI
                     ANPATARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEE
                     IVNYFPSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELP
                     ASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISG
                     SHKAEINAAIDSLDAEGSTNGGAGLEMAYQQAAKGFIKGGINRILLATDGDFNVGIDD
                     PKSIESMVKKQRESGVTLSTLGVGDSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLN
                     SEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLKAEDFNNDNVDAGDIGAGKH
                     ITLLFELTLKGQKASIDKLRYAPDNKSAKSDKTKELAWLKIRWKSPQGKESQLVEFPL
                     ASAIKAPSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQKAKGEDPQGYRAEFI
                     RLIGLAKDLDNSQN"
BASE COUNT         4262 a         4982 c         4681 g         4575 t
ORIGIN      
        1 tattcaatga cggatggaaa aacaatgtca ggatttttgc ctttttcgcg accagcaatg
       61 ggcgtagagg aactcgctgc agttaaagag gttctcgaat ccggttggat cacaaccggt
      121 ccgaagaatc aggcgcttga acaagctttt tgccagttga cgggaaatca gcatgccatc
      181 gcggtcagtt cagccaccgc cggaatgcat atcacgctaa tggcgttgga aattggcaag
      241 ggcgatgaag tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg
      301 ctgggtgcaa cgccggtaat ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa
      361 gcgatcgaag cagccattac accacgcact aaagccatca ttccggtgca ttatgccggt
      421 gcgccagcag atattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc
      481 gaagatgctg cccatgccgt aggtacgtat tacaaagggc gacatattgg cgcaaaaggt
      541 accgctattt tttcgtttca cgccatcaaa aatattacct gtgcggaagg tggcctgatt
      601 gttactgata atgaaaatct tgcccgccag ctgcggatgc tgaaatttca cggtctgggt
      661 gtcgatgcct atgacagaca tacctggggc cgtgcaccgc aggctgaagt cttaacgccg
      721 ggctataagt acaatctgac cgatattaac gccgcgattg ccctgacaca gttagtcaaa
      781 ttagagcacc tcaacacccg gcggcgcgaa attgcccagc aatatcagca agcactggca
      841 actctcccct ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg
      901 tttattattc gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg
      961 ttaaaagaaa gaggcattgg taccgggtta catttccgcg ctgctcacac acaaaaatat
     1021 tatcgcgagc gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc
     1081 tgttcattgc cgctgttccc ggatatgacc accgccgatg ccgaccgcgt catcacagcc
     1141 cttcagcaac tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg
     1201 gtggttattc ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc
     1261 gcagcctgtg aatcactggg gaaagagtat gaaatcctgc tgattgatga cggcagtagc
     1321 gataattccg cgcatgtgct ggtcgaagcc tcacaagcgg agggcagcca tattgtgtct
     1381 attttgctta accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtc
     1441 accggcgact taattattac cctcgatgcc gatctccaga atccgccaga ggagatcccc
     1501 cgtctggtgg caaaagccga tgaaggttac gacgtggtag ggactgtacg ccagaaccgc
     1561 caggacagct ggtttcgtaa aaccgcttcg aagatgatta accggcttat tcagcgcacc
     1621 accggcaaag cgatgggtga ctatggttgt atgctgcgcg cctatcgccg tcatattgtc
     1681 gatgcgatgt tgcactgcca tgaacgcagc acctttatcc cgattctggc gaatatcttc
     1741 gcccgccgtg ccattgaaat tccagtgcac catgccgagc gtgagtttgg tgaatccaaa
     1801 tacagcttta tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg
     1861 ccgctacgta tgttgagttt gctcggcagc attattgcga ttggcggttt tagcattgcg
     1921 gtgctgctgg tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt
     1981 atgctatttg ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattgctc
     2041 ggtgaatata tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag
     2101 caagttatcc gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc
     2161 acgatatggg atgcctcggt attgaagccc tgctgtctgc cggttacgaa attagcgcca
     2221 tttttaccca tactgataac cccggtgaaa aagcctttta tggttcggtt gcccgactgg
     2281 cagcggaaag aggcattcca gtttatgcgc cggataacgt taatcatccg ctgtgggtgg
     2341 aacgcattgc ccaactatca ccagaggtga ttttctcttt ttattatcgc catcttattt
     2401 gcgatgagat tttgcagctc gctccgagag gtgcatttaa tctgcatggt tcgctgttac
     2461 caaaatatcg tggtcgcgcg ccgctgaact gggtgctggt gaacggtgaa acggaaactg
     2521 gcgttacatt gcaccgaatg gtgaaacgtg ccgatgccgg ggccattgtg gcacaactgc
     2581 gcgttgccat tgcgccagac gatatcgcca ttacgctgca tcataaattg tgccatgccg
     2641 cgcgccagct actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg
     2701 cccagcgcga aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttcctcg
     2761 aatggcataa gccggcatcc gtactgcaca acatggtgcg tgccgttgcc gatccgtggc
     2821 cgggtgcctt cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc
     2881 ctcatgccag caaagcactg ccggggagcg tgatttctgt tgcaccactg ctgattgcct
     2941 gtggcgatgg cgcgctggaa atcgtcactg gacaggcggg cgacggcatt actatgcagg
     3001 gctcgcaatt agcgcagacg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg
     3061 cctgtgccgc ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca
     3121 accatctgac agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg
     3181 gcagcgatgc gataagccgt tttctgaatc atccgcattt tcactttgtc gaaggcgata
     3241 tcagtattca ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtgttgccgc
     3301 tggtggcgat agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg
     3361 atttcgaaga gaatctgcgc attatccgct actgcgtgaa gtaccgtaag cgaatcatct
     3421 tcccgtcgac ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggatcatt
     3481 ctaatttaat cgtcggcccg gtgaataaac cacgctggat ttattcggtg tctaaacaat
     3541 tacttgatcg ggtgatctgg gcctatggcg aaaaagaagg tttacagttc accctcttcc
     3601 gcccgtttaa ctggatgggg ccacgactgg ataaccttaa tgcagcgcga attggcagct
     3661 cccgcgctat tacgcaactc attctcaatc tggtagaagg ttcaccgatt aagctgattg
     3721 atggcggaaa acaaaaacgc tgctttactg atattcggga tggtatcgag gcgttatacc
     3781 gcattatcga aaatgcggga aatcgctgcg atggcgaaat tatcaacatt ggcaatcctg
     3841 agaacgaagc gagcattgaa gaactgggtg agatgctgct ggcgagcttc gaaaaacatc
     3901 cgctgcgcca ttacttccca ccgtttgcgg gctttcgtgt tgtcgaaagt agcagctact
     3961 atggcaaagg atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccgccgct
     4021 gcctggactg ggaaccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct
     4081 tcctgcgcac cgttgatctt acggataaac catcatgacc aaagtcggct tacgcattga
     4141 tgtcgatacc tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa
     4201 gcataatatt caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct
     4261 ctggcgactg gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct
     4321 ttatggctgg gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc
     4381 caatgccgat atcattcgtg aagcggctaa acatcacgaa gtcggcctgc acgcctggga
     4441 tcaccatgcc tggcaagccc atagcggtaa ttgggatcgg caaacaatga tcgacgatat
     4501 tgcacgcggc cttcgcaccc tggaagagat tatcggtcaa ccggtaacct gttctgccgc
     4561 tgctggctgg cgtgctgacc aacaggtgat cgaagcaaaa gaagcgttcc atttgcgcta
     4621 caacagcgat tgtcgtgggg cgatgccgtt tcgtccattg ctcgaatcag gaacccccgg
     4681 cactgcgcaa attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa
     4741 agcagaagat tttaacggtt ggttactcaa ccgcatccag cgagataaag gcacgccggt
     4801 ttataccatt catgccgaag ttgaaggctg cgcttatcag cataattttg tggatctcct
     4861 gaaacgcgca gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagggac
     4921 gttgccgctc ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg
     4981 ccaacaaatg gcgggtagtc actgatgaaa tcggtacgtt accttatcgg cctcttcgca
     5041 tttattgcct gctattacct gttaccgatc agcacgcgtc tgctctggca accagatgaa
     5101 acgcgttatg cggaaatcag tcgggaaatg ctggcatccg gcgactggat tgttccccat
     5161 ctgttagggc tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg
     5221 caatggctat ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg
     5281 ttaactgccg cgctgatgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct
     5341 ttactcgcca cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc
     5401 gtgctcgatc cgtttattgc cttctggctg gtggcgggaa tgtgcagctt ctggctggca
     5461 atgcaggcac agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc
     5521 ggcatggggg tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg
     5581 ccatgggtag caacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt
     5641 atcagttgcg tactgacggt tctcccctgg ggactggcga tagcgcagcg ggagcctgac
     5701 ttctggcact attttttctg ggttgagcat attcaacgct ttgcactgga tgatgcccaa
     5761 catagagcgc cgttctggta ctacttgccg gtcatcattg ccggtagcct gccgtggctg
     5821 ggattactcc ccggtgcact gtacgctggc tggaaaaacc gcaagcattc cgcaaccgtt
     5881 tatttgttga gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggcaaattg
     5941 cccacctata ttctttcctg ctttgcacct ctggcaatgc tgatggcgca ttacgctttg
     6001 ctggcagcaa aaaataatcc tctggcgttg cggattaatg gctggattaa catcgctttt
     6061 ggcgtcactg gcattattgc cacatttgtg gtctccccct ggggaccaat gaacacaccg
     6121 gtgtggcaaa ccttcgagag ctataaagtc ttttgtgcct ggtctatttt ttcgctatgg
     6181 gcatttttcg gctggtacac cttaacaaac gtcgaaaaga cctggccttt tgccgcgctt
     6241 tgcccgctgg ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga
     6301 aaacatccgc aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt
     6361 cttactgata gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc
     6421 atcatgtatc gccagacagg tgagttgaaa tatggcctta attatccgga tgcaaaaggg
     6481 agatttgtca gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt
     6541 attacactcg tgctttcggt tgaccgcgat gaagatatca atagtctcgc cattccgccc
     6601 gctgatgcca tcgatcgtca gggacgtctg gtactcattc agtatcggcc caaatgatct
     6661 ggctaacatt agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg
     6721 caacctgctt tgcggcggta aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg
     6781 cgctggcttg tcttggcctt gccatggtgc tctggctgct ggtcttgcag aacgtaccgg
     6841 taggcattgc ctacccgatg ttaagtctga attttgtctg ggtaacgctg gctgcagtaa
     6901 aactgtggca cgagccggta tcgctgcgtc actggtgtgg ggtggcgttc attattggcg
     6961 gcattgtgat cctcgggagt acggtgtaat gggcctgatg tggggattat tcagcgtcat
     7021 tatcgcctcc gctgcgcaat taagcttggg ttttgcggcg agtcatctgc cgccaatgac
     7081 gcacttatgg gattttatcg cggcgctgtt agcttttggc ctggatgccc ggatattgtt
     7141 actcgggctg ctgggttatc tgctttccgt tttttgttgg tataaaacac tacataaact
     7201 tgcgctaagc aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc
     7261 aatggtactt cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg
     7321 tattatgagc gggttgatgc tgattttcct gcccacaaca aaacaacgtt actgagtttt
     7381 ccctgccgct ttacactgcc gttcccactc atcggggcta taacagcttg cagaaagaac
     7441 tttcaccgtg tgcagctttt cacgattaat acagtaaagc gcattctgca aaggcgagag
     7501 taaatccccc actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc
     7561 gccagcatcg cagagcataa gtacatgtct gttattttgt ttattgcagc acgatttttt
     7621 gaccagccat tccattgcat tatcctgttt gataagagtt ttcacatcaa tttacagatt
     7681 aacagataaa cctattatca acggcaatgg aatggcaaaa ttgcgaagtg aaactaatga
     7741 tttcttaatc ctggcgttgc acccactctt ttaacgcctg acgtgaaatt ttaataccgc
     7801 cgtttttcag ctccggcggt aaagtcagcc agcgcaccgg ttgttgaaaa cgggccagct
     7861 tatctttcac ccattcgcca agatcaacgg tttgctggtc atactccacc accgctaccg
     7921 gtcgatgacc aaactccttg tcagcaacgg gaacgataaa cacctgcaaa accgcaggat
     7981 gtgcagcaat aacgcgctcg acttcttctg gctgaatgcc ttcgccacca ctgaagaaca
     8041 gattgtctaa acgtccgaca atggtcagct tgccattatg catctcaccg cgatcgcgcg
     8101 tggcgtacca gccttcgtca ttaaccagtg gaacccgttg cccgttacgc cagtaacctt
     8161 ctgccatgct ggcagcccgc agccacactt cgtcgtttac gattttcact tcccgacccg
     8221 gcagcggcga accgacgtct gccagaccgt cggcttcttt cgcacacacc gtggaggcaa
     8281 actcggtcag accatagccg caaaagcacc gaatcccctg ctcgcgcgcc tgttccgtca
     8341 actcgaccgg gatagccgcg ccgccaagta aaaccgcttt cagggaaacg gaactacggt
     8401 taaccagcaa acgccagagt tgtgttggca ccagtgaagc gtgcgtacag cctgccagca
     8461 tttgctccaa tggctgttta tcacgtaccg tcatccgcgc accagcgtat aaccagcgcc
     8521 acataattcc ctgaccggag acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat
     8581 cgccaaacgg aatcagcgat aacacccctt cggcgctggc aagatgagcc tgataagtat
     8641 gaacagcggc tttcggcaag ccagtggaac cagaggttaa cgtcattgag cacagacgcg
     8701 tcggctgcca cgccacggca tgtacgcctt caacctgctg aatatgcagc gacgttaacg
     8761 ccggaaacgt gttttcccct tccggcacca gcgcaaattg cagcgtaaga ttgggcagca
     8821 attcctcaag cagcggttgc ggcagctgag ggttcacggg caacacccgc gccccgcatt
     8881 gcagtaacgc cagccaggcc agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc
     8941 cgctgcccgc aaccacaccc tgcgccgcaa agctggaggc taattcatcg acgcgagcac
     9001 aaagctcgcg ccagttgagt tgctcgtcat taagacgtaa ggcgatggct tctccccgca
     9061 cttgccgcca gtgacgccac ggccagtcag agaagatcat aacagccgct ccagtgcatc
     9121 aacgtccact aacggcagtg gactccccgg ccagcgacga acctgctgca cctgcatcag
     9181 atccagcgtg tccagtcccg gaatggtgtc tggcgttaac caggcagcaa tccgcgccag
     9241 ttgcattaga cctaagctcg actcaatgga agaactgatc accgccgtca gccccagcgc
     9301 atgcgccgcc ttcacctgct cgcgtacttt ctccaggctg cccgtgagcg tgggtttgat
     9361 aactaccgcg cgcacgccct cttcagctac aaaggcaaaa tccggctcgc gcaagctttc
     9421 atcccaggca atggcaatgc cggtttcacg ggcaaacgca cgcgaatcat cgcgggtttt
     9481 gcacggctct tcgagaaatg cgatgcggtg gcgataatcc gggttaacgt atttggcaaa
     9541 ctgctggcct ttcagcggtg tccaggcgcg attcgcgtca agacgcaaat gcagatccgg
     9601 aattgcctcc agcaacagat tcaccaccat gccgtcgcgc acggcttcat acaatcccac
     9661 tttgaccttc gccactttct cgccaggcat atcggcaagt ttgaggatca gatcgtcagg
     9721 atcgccatta cacagcggtg ccgcacggta attggccgcc tgcgggagcg tatctgccag
     9781 ctccgccaat gcacaactta cgccaaaggc aacggaaggc atctgcggta attcgcaatc
     9841 gcctgccagc cagttattta cccaggcaag cagcacactt tgcgcatctt cccaggtttc
     9901 ctgactgaag cccggcagtg gggagatctc cccccaccct tcgcgctcac cctcacgcag
     9961 gcaaacatac agcccgtcgc gggtttttaa ccgcctgtcg cgcagaacca cccccgcgtc
    10021 catggggatc tgccagcggt atacctgcgc gctacgcatt acggattccg tttaaatttg
    10081 ctgaaatcag gctgacgttt ctggttgaaa gcattgcgac cttcctgacc ttcttccgtc
    10141 atgtagaaca gcatggtagc gttgcccgcc agctcctgca gccccgcctg cccgtcacag
    10201 tcggcgttca gcgcagcttt caggcagcgc agcgccatcg ggctgttttg cagcatttca
    10261 cggcaccaac ggacggtttc tttttccaga tccgccagcg gtaccacggt gtttacaagg
    10321 cccatatcca gcgcctgttt cgcgtcgtac tgacggcaca agaaccagat ttcacgcgct
    10381 tttttctgtc cgacgatgcg agccatgtag gaagcgcccc agccgccgtc gaaggaaccg
    10441 actttcgggc cagtctggcc gaagatggca ttatcagccg cgatagtcag gtcgcacatc
    10501 atgtgcagaa cgtgaccgcc gccgatggag tagccagcca ccatcgcgac aaccggtttc
    10561 ggacaggtac ggatctgacg ctggaagtcc agcacgttca ggtgatgtac gccggaatca
    10621 tctttatagc cgccgtaatc gccacgcact ttctggtcac caccggagca gaacgcttta
    10681 tcgcctgcac cagtcagaat gatcacgccg atgttgtcgt cataacgcgc atccgccagc
    10741 gcctggatca tctcttttac cgtcagagga cggaaggcat tgcgcacctg cggacgatta
    10801 atggtgattt ttgcgatacc gtcggtggat ttttcataac gaatgtcctc aaaaccttcg
    10861 gagcagtcgt gccattcaac cggtgcgtaa agcattgctt catcaggata aatcatattg
    10921 tgtcctttag tcagaaacgc aagatctgcg ccagacttgc gattacgcca gcgggatttt
    10981 cccggtgcgc gttatgtccg gcgcgaggaa tgacatggca gtcggcagcc agttccgccg
    11041 ccagagcgcg gaatttgctg tcacgttcac cacataaata ataaaacgca aatgtgcggg
    11101 cgctaaggtt agcacgtaaa tcgggctgga cggcgagaga agtcgcctcc agcatggcgg
    11161 caagggttgc gccattattg ttgctgcgca gcgccaccag ctcccggcgt tgatcgtcat
    11221 tgagcgaggc aaaaacaggc tgttgatacc aatcggcaaa taccgccgtt aacggttctg
    11281 tacgaaaacg ctgcgcccat tggcgatcgg aacgctgacg ttccgcgcgt tgttcagcat
    11341 tttgcaaccc aggatgcccg ccttcgacaa caactccaca aagccccgcc agctcctggc
    11401 aagccgccat catcgccacc cgtccgccaa gcgagtaccc caccagccag aagttaagga
    11461 tgttgtaact aaccagcgtt ttacacagta agtcggtgac atcatcaaat ccatcgacgc
    11521 tgatcgtcgc tgaaccaccg tgacctggga gatcaacata caaccgcgag tagtcggcaa
    11581 acgcctcgcc cacttcttgc cattcatggc aatcgccgga aaaaccgtgc aaaaacacca
    11641 gccagggtaa acctggtttg ccgtgttttg cctgcgcgtg caggatcata aatggcttac
    11701 ctgcgccaga agttgctgga gcgtttgcgc accatcggtg tcgttaacca ccatttcaat
    11761 caccgtggtg gtcggcgtac gccaggcatc gactagtgtc gtttcaagtt cctgccagtt
    11821 ttgcggacga tgatatttca gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg
    11881 cggcatcaga tagaaacgcc cgcgctcgct tttcggcgtt ggcaacagcg aaaaaatttg
    11941 cccgccgttg ttgttcacca caattaatac cagcggtgcg gaaacctggc gcaataacgc
    12001 cagcgcgttg agatcgtaaa gtgcggagag atcgcccaca atcgccagcg tcggtttgcc
    12061 gcttgcccgc tgaacgccgg cggcagtcga gagcagcccg tcgataccgc tggcaccacg
    12121 gttgctgtac accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgcaccac
    12181 caggctgtta ccgacaaaca attgcccctg ttcaggcaaa tagtcgctga tgcgatgcgc
    12241 cagttgcgct tcgccaaacg catcgcgacg ggcaataacc gcctgcattg cctgttccgc
    12301 caggcgcggg atttcaacgc accagggctg gcgtttttct gccggatgca gctccagcca
    12361 gtcggcaata ttggcaatta agcgacgtcc gcgatggtgt gccggatcaa gtcgcccttc
    12421 aatgtcatca acaatccagt actcttctgg ttcacagctt gcctgccatt ggaggagtcg
    12481 tttccccgtc aggctgcttc ccaattgcac cactatttgt gcctgttgca gttcgctggt
    12541 cgctttggca ttgcctaacc agagatcggc acacggcagc ggctgtccgg tttgcgacag
    12601 cacgtcgcca atcagcggcc agccaagagt ttgcgcccac agtgcaactt ttttgccctc
    12661 ttccgcactc atgcgcccgg caaccaccac accgcgcttt tgtcgccaga agaaccagtc
    12721 gcgctgtttt tcactttccc gacgaggcgc ttcacgcagc cacggtttgt cgtcctgcca
    12781 ccagtcgccc agccgctgtt gccagctaat ccccgtgtcg tccatttcgc catacagcgg
    12841 ttcagcaaac gggcagttga tatggactcc accagcatga agcgtaccga gagcgtggtc
    12901 gatggtagaa accagccaac gtgcggggat atcctgggtc gggcgcggca gtgaaatact
    12961 gtgcgtgggg tgagaggcga acattcccgg ctgacgaatc gcctgattcg cgccgcagtc
    13021 aatcagctcc ggcgggcgat cggcggttaa caggatcagt ttttctccgg ttaacccagc
    13081 ttcaatcagc gccggataga gatttgctac cgccgtgccg gaggtcacaa tcaccgccac
    13141 cggctgcttg ctgactttcg ccagccccag cgccagatgc cccagtccac gctcatcgaa
    13201 atgggtgtgg tgaatgaatg cggaattctc cgccgccgct aacgttaacg gtgtagaacg
    13261 cgagccaggg gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac
    13321 cgccgcccag cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc
    13381 ggctaagtat aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc
    13441 attactattc catttgtaat aaagtacgca gccctgccgc tttgttgtcg atttcctgcc
    13501 actcttgctc ggggtcggaa ccacggacaa tgcctgcgcc agcatatagc cgcacgatat
    13561 tgccgctgat ttttgctgag cgcagggaaa cgcagaattc actttgctgt aatgaaagat
    13621 agcccgcaga accggcgtac cattctcgag tgaacggttc gtgacgggcg ataaactgtc
    13681 gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga
    13741 tcacatcatc cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgtactttac
    13801 gtagacgcag tacctgcggc ggtaaaacat ccagcgtccg ggtatcggcc tgcaatcgtt
    13861 ggcagatatc ttccaccacc aacatgttct cgcgctggtt tttatcatcc gccatcagcc
    13921 actcacctaa ctgctgcgcc tgcttatcat caggatgatt tgctaccgtt cccgccagcg
    13981 cttcggtacg cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gaaccaagaa
    14041 aagcattttc gccatcaaag gccatgtaaa aatggtagca attcagattg agccgacgac
    14101 tggcagccat catcgctgcc gcattgaccg gacttgcgaa atgcaggtca gttgcccgag
    14161 caagcacaac tttgtcgagc tcgccttcgg cgatggtttt cgttgccagt tcgattaata
    14221 gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa tgtaatccag
    14281 gcaggggctt gatactcacc agtgtggcga taaattcttt tgccttgatt gcatcgtgct
    14341 gaagggagct ttcgctgaat agcgtcaacc gcagcgtggc tttaccgcca caacgtcgcc
    14401 attccaggcg tggtaaaagt aaatagccct gcgacgggtc aaacgcattc aacccccaga
    14461 tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg
    14521 taaaacgggt aatcgcaccc aggacggcag cttcttcatc accattacgt tgttgccagt
    14581 agaattgcgg atacacctgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga
    14641 gagggaaagg aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat
    14701 ggcgcagtag attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa
    14761 agcctcacat tatacggggt acgacaaaaa aaatgcagta ccccggtgta gggaggtttt
    14821 aacggcgtgc cagcaacagt cctagaacca gcccaacggc cgcacccaca ccaattcctt
    14881 gccagggttt ttcgtggacg tagtcatcag cacgataaac tgcctgcttc gcccgatagt
    14941 aataactgtc tgaagcctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcac
    15001 gcgctttcag ctcaacatat ttctgatcgg cgggatcgcc agaggagcgg agcacctctt
    15061 ccagtgtttc actaagcagc gtcaggtcgt catcaatacg tgtatcacca aactgattag
    15121 acattctcgt tctccatttg tgtaaaacct gtcactaact atagacaagg gttattgatt
    15181 acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcataaatat
    15241 cggtcacagg gatgaaacca aattgcccat aaaagttttg taaatgcgcc tgcgccccca
    15301 gataaatcgg cttctccggc cagcggcgag tacacgattc cagcgcctta ctcatcaatt
    15361 gctgaccgat tttttcaccg cgtaaggctt cgctgacaat cacccgacca atcacaacgg
    15421 gttgcagttc gtcgtcactt ttcagaatcc tcgcatacgc caccagtgtc ccgttatgcc
    15481 aaccgagaat gtggcgattt tctccctcca gatcgtcacc atcgatatcc tggtaaggac
    15541 agttttgttc cactacaaat accgcacaac gcagttgtaa taaggcataa agctgagaaa
    15601 cggataaatc agaatgatgc agatcttgcc agtcgatcat ggaaggctcc tttgtgctgt
    15661 ttgctttata ctagccggaa tattttcagc agcgtgatga actcatggaa ttaatttttt
    15721 taggtacttc agccggtgtg ccaacccgta cgcgtaatgt cacggcaata ttgcttaacc
    15781 tgcaacatcc gacccagagc ggactttggt tatttgactg cggtgaaggc acccagcatc
    15841 agctactgca tactgccttt aaccctggaa aactggacaa gatttttatc agtcaccttc
    15901 atggcgatca tctctttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta
    15961 tccaaccctt aacgatttat ggtccgcacg gtatccgtga atttgtggag accgcgctgc
    16021 ggattagcgg ctcatggacc gattatccgc tggaaattgt cgaaattagc actggcgaaa
    16081 ttttcgatga tggcctgcgc aaagtgaccg cttatccgat ggaacaccca ctggaatgtt
    16141 atggttatcg tattgaagaa catgataaac caggtgcatt aaatgcccag gcattaaaag
    16201 ctgctggcgt gccgcctggc ccgctgtttc aggaattaaa agcgggcaaa acaatcatgc
    16261 tggatgatgg aaggcagatt aatggcgcag attacttagc cgctccagtg ccgggtaaag
    16321 cgctcgctat tttcggcgat accggcccct gcgatgccgc gctggagctt gctaaaggcg
    16381 tcgacgtaat ggttcacgaa gcgactctgg atatggcgat ggaggccaaa gccaacagtc
    16441 gcggccatag ttctacacgc caggcggcgg cactagcccg tgaggctgga gtcggtaagc
    16501 taatcattac ccacgtcagc tcacgctatg atgacaaagg ttgtcagcac ctgttacgtg
    16561 aatgtcggtc aattttcccg gcgactgaac tggcgaatga tttcgcggtg tttagcattt
    16621 aagtcgaaag cggtggttag ttagcagatt aatttaacga taccaacaca tttatttgac
    16681 attttttcat ctgtttgccg gatacgtttg atgtcgatat caggcaaaca gaattatcat
    16741 ttttagtttt gtgaattgtc cagatctttc gccagtccaa tcaggcgaat aaattccgct
    16801 ctgtaaccct gtggatcttc cccttttgcc ttctgagccc actgtttgat ctgctgccag
    16861 gaggtattgt tcaggtattc agaaccgcgt aacttttgcc catatgcagc cactgctgcg
    16921 cgaaagcgca tatcttctga aggtgccttt attgcagatg ccaacgggaa ttcaactaac
    16981 tgactttctt ttccctgtgg ggatttccaa cgaatcttta accaggcaag ttcttttgtt
    17041 ttgtccgatt tcgctgattt gttatccggg gcatagcgta acttatcaat tgatgctttt
    17101 tgccctttca gcgttaattc aaataacaat gttatatgtt tacctgcgcc aatatcaccc
    17161 gcatcaacgt tgtcgttatt aaaatcctct gccttaagtt ggcgcttttc ataacctatc
    17221 tgacggtatt ccgttaccca cgcgggatta aactcaattt gcgctttgac atcttttgct
    17281 acggtaatca acgtctgccg catttcactg ttcaacactt tctgcgcctc tgagagggta
    17341 tcaatgtagc tatagttgcc gttaccaaca tcggcaattc tcaccatcat tgcctcgtta
    17401 taattgctat cccccacgcc aagcgtcgac agagtaacac cggactcacg ctgttttttg
    17461 accattgatt caatcgattt tggatcgtca atgccaacgt taaagtcacc gtcagtggcc
    17521 aataaaatgc gattgatacc acctttaata aaccccttcg ctgcctgctg ataagccatc
    17581 tccagcccgg caccgccatt ggtactgcct tctgcatcca gcgaatcaat tgcagcatta
    17641 atttccgcct tgtgactacc ggagatagaa ggcaacgcaa tacgagagtc gccagcgtag
    17701 gtcacgatgg caatgttatc ctgctcacga agttctttga ccaataattt caacgaagac
    17761 tggataagtg gcaaacgttc atcagaaatc attgaaccag acgtgtcgat aagaaagacc
    17821 agattagaag cgggtaactc ttcacttttg cgatctttcg ccaggatatc aactttcagc
    17881 aatgttcgct gttcattcca gggtgcaggt gccaattcgt agcgcatagc gaaaggtatt
    17941 ggcttagagg ccggaataga ttgtttgtct ttgatatccc agtcagacgg gaaataattg
    18001 actatctcct ccacccgcac agcgtctggc ggaggtaaca gcccttgatt aaggaaacgc
    18061 cgcacattcg cataactgcc ggtatcgacg tcaaggctaa acgtcgccaa cgggttttgc
    18121 gctacctgct taaccggatt atcatcgaac tgctggtagc gagcggttgc tggatttgcg
    18181 atttgggagg ctgctttttc tctggctgca tgttgatatt ttggagccgc ctgcaatcgc
    18241 cccagcaaag cctgtttgtc tgaatattgt tgcacttctt gctgggctaa ggctttcgcg
    18301 tctgcagcgg ctttggcggc gctttgctca gccacttttg ttgccgcttg ttgcgcggca
    18361 agcacttgct ggtcagtggg tgtagtagat tgttgttgct gacttttctt atcctcaggt
    18421 tccggcccac atcctgacaa aatcaaacta cttataagca acatgattat atttttattt
    18481 cgcatataaa atccttttaa
//
LOCUS       DACADF010000088        18441 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.089, whole genome shotgun sequence.
ACCESSION   DACADF010000088 DACADF010000000
VERSION     DACADF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.089"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(468..1790)
                     /gene="hipA"
                     /locus_tag="ISB30_002993"
     CDS             complement(468..1790)
                     /gene="hipA"
                     /locus_tag="ISB30_002993"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HAP1990602.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     QIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHDRLSREYGIK"
     gene            complement(1790..2056)
                     /gene="hipB"
                     /locus_tag="ISB30_002994"
     CDS             complement(1790..2056)
                     /gene="hipB"
                     /locus_tag="ISB30_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HAP1990603.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            2364..3122
                     /gene="tam"
                     /locus_tag="ISB30_002995"
     CDS             2364..3122
                     /gene="tam"
                     /locus_tag="ISB30_002995"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAP1990604.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYIADLGCGPGN
                     ITALLHQRWPAARITGIDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAIQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(3126..4040)
                     /locus_tag="ISB30_002996"
     CDS             complement(3126..4040)
                     /locus_tag="ISB30_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990605.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIVFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(4237..5688)
                     /locus_tag="ISB30_002997"
     CDS             complement(4237..5688)
                     /locus_tag="ISB30_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP1990606.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGVRNGETYPVQDDAHWLERYQQLWSQYRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(5915..7333)
                     /locus_tag="ISB30_002998"
     CDS             complement(5915..7333)
                     /locus_tag="ISB30_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAP1990607.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDTLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(7472..7831)
                     /locus_tag="ISB30_002999"
     CDS             complement(7472..7831)
                     /locus_tag="ISB30_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAP1990608.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(7831..8757)
                     /gene="glsB"
                     /locus_tag="ISB30_003000"
     CDS             complement(7831..8757)
                     /gene="glsB"
                     /locus_tag="ISB30_003000"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAP1990609.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(8821..10209)
                     /gene="sad"
                     /locus_tag="ISB30_003001"
     CDS             complement(8821..10209)
                     /gene="sad"
                     /locus_tag="ISB30_003001"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HAP1990610.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLVAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEDNALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRV"
     gene            10310..11191
                     /locus_tag="ISB30_003002"
     CDS             10310..11191
                     /locus_tag="ISB30_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1990611.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVESWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            11269..11352
                     /gene="yneK"
                     /locus_tag="ISB30_003003"
                     /pseudo
     CDS             11269..11352
                     /gene="yneK"
                     /locus_tag="ISB30_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            11530..12720
                     /gene="ydeA"
                     /locus_tag="ISB30_003004"
     CDS             11530..12720
                     /gene="ydeA"
                     /locus_tag="ISB30_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAP1990612.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALLTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(12745..13410)
                     /gene="marC"
                     /locus_tag="ISB30_003005"
     CDS             complement(12745..13410)
                     /gene="marC"
                     /locus_tag="ISB30_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAP1990613.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EVKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            13622..14056
                     /gene="marR"
                     /locus_tag="ISB30_003006"
     CDS             13622..14056
                     /gene="marR"
                     /locus_tag="ISB30_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAP1990614.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            14077..14460
                     /gene="marA"
                     /locus_tag="ISB30_003007"
     CDS             14077..14460
                     /gene="marA"
                     /locus_tag="ISB30_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAP1990615.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            14492..14710
                     /gene="marB"
                     /locus_tag="ISB30_003008"
     CDS             14492..14710
                     /gene="marB"
                     /locus_tag="ISB30_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAP1990616.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(14741..15640)
                     /gene="eamA"
                     /locus_tag="ISB30_003009"
     CDS             complement(14741..15640)
                     /gene="eamA"
                     /locus_tag="ISB30_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAP1990617.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLTGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFAASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            15835..17022
                     /gene="ydeE"
                     /locus_tag="ISB30_003010"
     CDS             15835..17022
                     /gene="ydeE"
                     /locus_tag="ISB30_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAP1990618.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTFCFVIGLVGFIFSGNSLLMWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQP
                     ALC"
     gene            17149..17244
                     /gene="mgtS"
                     /locus_tag="ISB30_003011"
     CDS             17149..17244
                     /gene="mgtS"
                     /locus_tag="ISB30_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAP1990619.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(17463..18353)
                     /gene="dgcZ"
                     /locus_tag="ISB30_003012"
     CDS             complement(17463..18353)
                     /gene="dgcZ"
                     /locus_tag="ISB30_003012"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HAP1990620.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTVRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         4803 a         4667 c         4266 g         4705 t
ORIGIN      
        1 tacctgtttt aaacgatata tttggaagtt gacgaatatc ggaaatagct tcctccatca
       61 gctgtagtca ctgttctctg tatttcacat tgcatatcaa cacgatatgc tgagattagc
      121 taacttgaga ttaaagataa ttacacggcg ctatgaatta tattcagaag cgtgagggat
      181 atagtgccat caaagcgctg gctgctgtaa acaacgcaat tatccagctc atggtctatg
      241 gtgtgccatc actgaacaga ggttgatttt tctaccgaaa cgggagggaa gtcagaaatc
      301 acaacgtatt gatttatctg tgtgtggatg tgaatggtga agtaaaggac cttcataatc
      361 aatttaacat aatatacatt atgcgcacca atataaacca agggcgaatc cagtcgttgg
      421 cggttcatga ttgttctgct cattaacaac gaccaaaacc ccatatctta cttaataccg
      481 tattctcggc ttaaccgatc gtgcaacctc aacacattgg tttcaactgc cgtcaccacg
      541 ttctccggaa aatctgtcgg taatgaagtc ttcacgttat ccagtgctgc cggaatcatt
      601 ctggcaaagt cactcaggat ttcatgcatc tgcacttccg ggaatttcag cacctttgct
      661 gtcgccagaa aatgtcgcgg ataaatttta tcgattgccg tttttttgcc tttggatgcg
      721 ttaagcccca ttgccagttt gagatcgctg atgtgtattc ccgtaccgcc aaggaccgga
      781 aatgccgaaa tgatgtcgta aaatggtgtg agccgataac tgccgccagc ctgaataaat
      841 acggagaagt tttttgcatg gccatccgtt gcgccaatca accactggaa gacctgaaac
      901 ttcataaaat catagcgatc tttcagcgcc tcgctggacc ccatcaaaaa agccataatt
      961 tgcgcgatac cggggcctcc atctgattca tatttcaccg atgaaggtaa accgaatgtc
     1021 tggcacatat cctcctgtgg caagcgaagt aaaaccgttc gctcagtatt ccaacgcctg
     1081 tcaaaacgtt cgaccgctaa cgcgcgcacc cttcccgctt taatgatttc tgcgtccgga
     1141 acattcaacc cgagttcttt cgccagcagc aaacagtaat actcattatc aacgctttgg
     1201 ctaagatcga gcgtcgcatt gggctgtctg atttcgccaa tcggcaattt gataatgtgc
     1261 gtcgttggcg ttattccctt cggaatgcac cagtcattgc ctattctgag cagcgcagtc
     1321 ttctcctgtg cgccagcaac cgagatgcga aagtcatttt cttctctaat catgcctaac
     1381 gggatatctg ctttataagc cgttaatact tcttcaagtc tggcttcagt aagcttttcc
     1441 catgccatta tcggacacgt tacggtttca tcttcgggga ttaacgtcac ggcaccaacg
     1501 ctgtctcgcc ctatttctga caataaatca aacggttgtc tggatttagc atgataacgt
     1561 ttaacgatcc ggtcacgtac aatcgggcta tcgggtaaca ggttatcgaa gaagttaaat
     1621 acggcatcag aggtgatatt ccccctctgc aatggcagcg aaagtgacaa cggtctggca
     1681 taacggctgg ctaaccactc agctgcatac ttaaaggtgt gcgcgccgtt ggctaacttc
     1741 gttaactcgc ctacccgctg gttgttcatc caagtgacta gtttaggcat taccactcca
     1801 gatcttgctg ttctgttgct tctggcgagg catttttcgc gtcgcatagc gtcattgaga
     1861 gttcaagcga ctgtaaaatc ttaaaaaatg tcgtgagcga ggtattgtca ggattgtttt
     1921 cgaaattgga aattgtcgcc tgcttaatgc cgattttttt cgccaactcg ctctgcgtcc
     1981 agccgttttg ctggcgaacc agtttcattg cattcgccaa ttgcgttggg ctatagatct
     2041 tctgaaagct catcatgtca tacgtccaca gcaaatttat ccgcttaagg ggatattatc
     2101 agttttatcc tttagtgagg ataaagtcaa tctggaaaca catcttatcc gctctacggg
     2161 ataaacgctg cgatatccgc gatcgcggat aaaaaggagg atttaaaaaa gcacatccta
     2221 atgcggtggc ttctttgact accggaatct gtaatcttaa tgaccgggcc gcgtaaaaaa
     2281 cgtctgttca atggggtgat gccgtgaggc gaatttatca gttttatcta caattggggt
     2341 aacgcgctga cgggagtaaa aaaatgtctg actggaaccc ctctttatat ctgcactttg
     2401 ccgctgaacg atcgcgtccg gcggtggagc tgcttgccag ggtgccgctg gagaatatcg
     2461 aatacattgc cgatcttggc tgtggcccag gtaacatcac cgcccttcta caccaacgtt
     2521 ggcctgctgc caggataacg ggcatcgact catctccggc gatgattgct gaagcgcgca
     2581 gtgctttgcc agactgcctg tttgtggaag cagatatccg caactggcaa ccggaacagg
     2641 cactcgatct gatttttgct aatgcctcac tgcaatggct gcccgaccac tacgaattgt
     2701 ttcctcattt ggtttcgtta cttaattcgc atggtgtgtt ggcaatacag atgccagata
     2761 actggctaga gccaacccat gtgctcatgc gcgaagtcgc ctgggaacaa aattatccgg
     2821 atcgcgggcg tgagccgctg gctggcgttc atgcttacta cgatattctg agtgaagccg
     2881 gatgtgaggt cgatatctgg cgaaccactt actatcacca gatgccgtcg caccaggcga
     2941 ttatcgattg ggtgaccgcc actggattac gtccgtggtt acaggatctg accgagagcg
     3001 aacagcagca ttttcttacg cgctaccatc agatgctgga agagcaatat cccctccagg
     3061 agaacggaca gatattgctg gcatttccgc gtctgtttat tgttgcccgc cgtacggagt
     3121 aattatcacg tcagctggta atggcgatcg ggaagaattt tcgctggaat ttctgcttca
     3181 tcattcatct gtaacaggtc aatttcaata gcgttgcaga tggcatccag cggtaaatca
     3241 ttgttttcag taccgaaggg atcttccagt tcttccgcca gacaatccag cgaaataaaa
     3301 gtgtaggaaa tcagcacaga gataaaaggc gtcatgtaat gcaggtccac gaccagcgca
     3361 aacggcaaca tgatacaaaa cagatacacg gtgcgatgca aaatcagggt gtaggcaaag
     3421 ggaattggcg tataggcaat gcgctcgcat cctgccagga ccgctgaaat atcattaagc
     3481 cgatcgttga ggctaataaa caggatatct gaaagttgtc cattgcgtcg ctgaaccgcc
     3541 aaccactccc ccattattaa caagatgcgg ttagctggag agttagaagc cagtacacgc
     3601 tgaagatctt cagtcttgag ataatgagcc agcacttctg cctgtggctg tttgcgtaat
     3661 gtcatgcgta aacagtgggc gaagacgatt tgcagccggg caaactccct tacacttgcc
     3721 gaatccggta atgtcgtttt tacctcgcgc agtaacgacc gcgaggcaat catcaactgc
     3781 ccccaaagtt ttcgcgcttc aacgtaacgg gcgtaaccgg cattattacg aaaaccaaga
     3841 aaaatggcga tggcgacacc gagaatgctg aacggtgcga gggtgaattt aatgcccaaa
     3901 tgcgtgtacc agggcagcat gaaaataaca gcgatcgaaa agagaaaatt gagtagtaag
     3961 cgcgaggata tcttggataa tactgaaccg tgccagacaa aaatacggcg cagccagtgt
     4021 tgttgtggac gaacaatcat ggttatcttc aggcgtggaa aagtcgccct attaaacgtg
     4081 attatgatca cattctcaag atactactta caaattacct acaagattac aacattaaca
     4141 ggtgctgcat gtggcacctg ttaatgataa aggcatatag aatgttgtaa ctaatatagc
     4201 ggaataagaa acccggtcga aaccgggttc agagtattag cacaacgggc gtacagcttc
     4261 gcgcatccct ttttcgagaa tcgcatccag gtcattagca acctgctcga ccagaccagg
     4321 cacttgcgtc aggtcctgct cccagtggtc tttttctgcc aacacaatcg ctaccagttc
     4381 ctgggtcccg ataacacggt cacgatactg gctccagagt tgctgataac gttccagcca
     4441 gtgtgcatcg tcctgtaccg gatatgtttc tccattacgc acaccgcgat agaacgcaat
     4501 caatgccgct aatgcgaaag taaggcgcgc cggaagtgtg ccgtttgcct tctgccctgc
     4561 cagcagctgc ggcaggatgc gtgtgcggaa cttggtcata ccgttgagcg cgatagacag
     4621 cagttgatgc ttgatgtacg ggttacggaa acgtccggtg actgcactgg cgaaagactc
     4681 cagctcatcg cgtggcaaat ccagtaccgg aataatttct tcgtagatgg ctttttcaac
     4741 gaatgcgcaa atttcagcat cgttcatcgc ttcacctacg gtatccagcc ctgcctggaa
     4801 cgccaccggc accagcgcgg tgtgcgcacc gttgaggatt gccactttgc gttctttata
     4861 cggtttaata tcgtcaacga tcagcacgtt aagcggatat ttgtccagac gaagttcagt
     4921 cgctaaggat ttcggcccct gaatcacaaa caggtaaaag tgttcagcgg tgtcgaggaa
     4981 accatcgtgg tagcccagtt cttcttccag tttagccact tcatcacgcg gataaccggt
     5041 aacgatacgg tccaccagcg tagaacagaa gctgttagcc tgatccagcc attgaataaa
     5101 tgcttcaggc aatgcccact cttgcgcata gcgcagcacc agttcacgca acgcgtcgcc
     5161 attatagtca atcaactcac acgggatgat gatccaacct ttatccagcg caccgttgaa
     5221 atggctaaaa cgttcgaaca gcagacgggt cagttttgcc ggataactta ccgctggcgc
     5281 gtcatcgaat ttatcgcccg catggtagct gatacccgct tcggtggtgt tagagaaaac
     5341 aaagcgcatt tccgggttgt gagccagttt caggaattca tcgtattcac tatagacgct
     5401 gatttcacga ttaaccgagc gaatcagacg cgcgtcgctg accgcttcac ccttctcatt
     5461 caggccacgg ataatggtgg tatacagacc atcctgcgtg ctcagtgacg gcgggaatga
     5521 agtttcaatc ggacgaacaa cgaccacgcc agaattcaga tcggtgtgct cattcaggag
     5581 atcgatttgc cagtcaacaa aggcgcgcag gaagttacct tcaccaaact gaatgatacg
     5641 ttctggatac tgtgcaccgg gaaaatcgcg acgatttagt gttttcacaa tgggttccct
     5701 tctgattagt catacaacct gtttgaattg gtacgacagg ttagcaaact ttaatacgtc
     5761 gaacccctgt tttgatcaac tcctgatgat taatgagcag ttttatgaga aaagtgtggc
     5821 gcggatcatg gtttaatcga ggaaacaggc cttttcttgg atcataaagt ggtagaacac
     5881 attgcattta attcgctctg agtaaataaa gatgtcagac aacttcctca ccgtaacgca
     5941 tagtgctggt acggttgcgc ccatctttct tcgagcgata cagacacgta tcggcttcga
     6001 ccatcaattt attaaagtca tcggtcagcg ttcggtacga tgcgcaacca ctaccgacgc
     6061 caatacttac cgtgagataa agcgtttttt gttgccaggt gaacggttgc agttcaacgc
     6121 ctttacggat tttttccgcc attagcaggc catcgacagg attcacagac ggcactgcaa
     6181 cagcaaattc ttcgccgccc attcgcgcca ccagcccctt atcaccgaca atcttctgaa
     6241 tatgctgggc aaacacgctt aacactttat cgccacattc atgtccatag ttatcgttaa
     6301 tgcttttgaa atagtcgata tcaagcagca tgaccgtcag atgttgtgtc tgtttcagcg
     6361 atggactttt cagcgcctca taaagaccgg accgtgagta aacctgggtc agaaaatcaa
     6421 aatcggctcg cagcgcaact tgcttcatta gcgaattgat cgctgccacg ctaaaggaaa
     6481 ccataattgg gcatatcgcc atcgtggcaa taccgagacg tgcggagaac atttgcggaa
     6541 tggagaacgg cgaaccgacc gagatatcaa tcaccgaatt tgccaccagt acgacttcca
     6601 cagcgccggt gacaaaggtc aacagacatg ttacttgcgg cgtatagcgc actgcacacc
     6661 agattaatgc agggagcgga aacgccagac tccccgctcc gccaatgact accgaggcaa
     6721 taaccgacac aataagcgcg atagcaggca tcatctgctc tgctttaaag cgcggcagca
     6781 ctccaggaat tgccaacgtc agcatacaag gcacgatcaa cacgcccgtt gagaattgct
     6841 cactaaacca gtcggcaagc aaaggccaga aatccagagt atcaatactg accgaaccaa
     6901 tcgccccgac aatagcgcat aataatgcgg caagcagaca gtaattaaat aaccgtaagg
     6961 cacttactgg ctcatactta ttttttccca gacgcttatc ccgcgcgacc agtaaggcga
     7021 cggtaacaat aaacatcata ttggagaaat tgatagccag tgaaaccagc ccccattcgg
     7081 tggttatggc atcataaacc agcatcgcca cataactgat cgcatagtaa tgcagtcgat
     7141 taagccagac atagcgggca aacacccccg ccattacgcc attcagcggc cagaaaagag
     7201 aaagttcgtg aaccagccgt aactctgcac caataaaata gaacagcgtg gttagcacaa
     7261 aaatagcgat gctattacgt agcagatgac cttcctggaa caaacggaaa gttgaaatgg
     7321 gctgtacatg cattaattat ctgtccatac aattgcgggt ggcaattatc tcaacgcaat
     7381 aagtaaattc ttagaaagga tagcatgttc gctgacaacg gcggcaacta aagataccta
     7441 atgaatgcca ttttatcgat ttccgtaatt atcatggctg attcatctgt ataaccagcc
     7501 agtgatagat taccgtcaca atataacgtt gttgctcctc acttaacgct tccccctgag
     7561 gaatgttatg ccacttagcc agacaaccgc gacaacaggt cgcggtggcg tgttgagcga
     7621 taaaaaccgg atgcccgcgc atgggggttt gcttaccgtc gttagccggt aatgccggag
     7681 caagacgttt agcgacaaaa tctgccgcat gttgttcaat gactggcgcg cctttctcca
     7741 gacaatactg acgctctttc acacccaaac gaaagcgaga gcgaaatttt gaacgggata
     7801 aacgcgcgaa gagtggatcg agagactgca ttaataaact gaacgcccta attgtttcgt
     7861 caattgttca aggacggcaa tgcccgcaag cgagttaccg gcatcatcca gttccggact
     7921 ccagacagcg atggccattt catgcggaac aatcgccaca ataccgccac caacgccaga
     7981 ttttgccggt agtcccaccc gccaggcaaa ctcccccgcg ttctggtaca taccgctggt
     8041 cgccatcagc gcgttaattt gccgcgcctg cattggcgtc accacaggtt catcaatatg
     8101 aatagctttc ccctgattcg ccagaaagac aaacgtccgg gccagctcta cacagctcat
     8161 tttcagagcg cagtaatgaa agtagttttg cagaacggtt gtcacgtcat gatggaaatt
     8221 gccaaacgac ttcatcagcc aggcgatagc cgcatttcgc gcggaatgtt caaattcgga
     8281 acgcgctacc accgtatcgt aggaaatatc agacacacca cttaagccgc gcacgacttc
     8341 cagcatacgt tgccgaggtg cgcttaatcg cccttgcaac atatcgcaga ccaccagcgc
     8401 accggcatta ataaacggat tacgcggaat accctgctcc atttccagtt gcactaaaga
     8461 attgaatggt gatccagacg gatctttgcc gacgcgttgc cagatttcct cttcggagta
     8521 atgacgcatg gcgacaacga gactcagcac tttggaaata gactgaatgg aaaaacgttc
     8581 ttgcgcgtct ccggcctgaa aaagctgtcc gtcaacggta cagatagcaa tccccaatcg
     8641 ggaaccgtct accgtagcca gcgccggaat ataatccgcg actttaccct gaccaatgag
     8701 cggccgcact tgccgcaaga tgttttctaa aattgcatta tccatggcga ctgccacttt
     8761 ctactcctgg accgcaggtc tgaaaagacc tgcgagtata tcagagctga atatatagcg
     8821 tcagacccgg tctttccaca ccgtctggat attacagaat tcgtgtaagc cgaaatggga
     8881 aagctcacga ccaaagccac tcttcttcac gccaccaaag gcgactcgcg catcgctggc
     8941 acaataacca ttgataaaca ccccaccgca ttccagacgt gccgccatct gtctggcctg
     9001 tgtttcgtcg gtggtgaaaa tggtcgctga aaggccgaac tcactatcat tagccagttc
     9061 cagcgcatgt tctgcatctt tcgcaatggt gattgccgca acggggccaa acatttcttc
     9121 ccgaaacgcg gtcatttctg gggtaacatt cgccagaacc gttggcggat agtagttacc
     9181 tgccccagcc atcttttccc cgcccagtaa caaacgcgca ccctgcgcca gtgttttttc
     9241 cacctgatga tgcagctcat cacgtaaatc aaaacgagcc attggtccga gagcgttgtc
     9301 ttcatcacgg ggatcgccca ttttcaaggc tgccgcagct gccacaaaac gttcggtaaa
     9361 tgccgaagca attccctctt cgatgataaa gcgttttgcc gctgcacaaa cctgtccggt
     9421 attctgataa cgtccggcta ccgccgcttt caccgccagt tccagatcgg catcgttaag
     9481 cacaataaac ggatccgaac cacccagttc cagtacgcat tttttcagtg ccgctccagc
     9541 ctgtgcgcca atagccgctc cagcacgaac acttccggtc actgtgacag cagcaatgcg
     9601 cgaatcttta atcatctgac tgacaccgtc gttgtcggca ttcagccagc catatacgcc
     9661 ttgtgggata cccgcatctt taaacacctg ggcaacgagc tgtgcacagc ccatcacatt
     9721 cggcgcatgt ttaagtaagt agccgttacc agcaagaatg atgggaacag cgccacgcat
     9781 cacctgccat aacggaaaat tccacggcat aatcgccaga atcgtcccca acggtcgata
     9841 ctcaataacc gcctgctgat tttccaccag cgtaggttct gccttcagca ttgccggacc
     9901 atgttctgca taccagtcac acaaattcgc cgatttcgcc acttcagcgc gcgcctggtt
     9961 gattggtttg cccatttcac gggtgatcat ttgcgccatt tcttcgctac gggcgcgcag
    10021 agccttaccg atatcacgca gtttttcagc acgataatct atatttgtct cgcgccagtc
    10081 gcgaaagcct gctgccgcca gctgaagtgc gttttcgata tcgtcaacac cagcccacgg
    10141 cagcacagaa agttgttcac cggtggcagg atttatcgaa attgcatgag ttgccggagt
    10201 aatggtcatc ggggtatctc ctttatgagt catggtatga agatacgcag atttactctt
    10261 gctttaaaat gaataatatt aagccactta ttcacgaatc gagaatgcca tggatctgac
    10321 ccaactggag atgttcaacg cggttgccga agctggcagc attacccagg ctgcagcaaa
    10381 agtgcatcgc gtgccgtcga atctcactac ccgtctacgt cagctggaaa cagaactggg
    10441 ggttgatctg tttattcgcg agaatcagcg tttacgtctc tcgcctgccg ggcataactt
    10501 tttacgctat agccaacaaa ttctcgcatt agtggatgaa gcgcggagcg ttgtcgctgg
    10561 cgatgagccg caaggtttat tttctctggg atcgctggaa agcaccgctg cagtgcgcat
    10621 tccggccacg ctggcggaat ttaaccgtcg ttatcccaaa attcagtttt cactttccac
    10681 cggcccttcc ggcactatgc tggaaggtgt gctggaggga aaactgaatg cggcgtttat
    10741 tgatggaccc attaaccata ctgccatcga cgggataccg gtataccgcg aggaactgat
    10801 gatcgtcacg ccacaaggac atgcgccagt aacccgtgcc agtcaggtga atggcagtaa
    10861 catttatgcc ttccgcgcca attgttcgta tcgtcgccac ttcgaaagct ggtttcatgc
    10921 tgacggtgcc gctccgggaa ctattcacga gatggagtct tatcacggga tgttggcctg
    10981 tgtggtcgca ggagcaggca ttgcgcttat tccacgctct atgctggaaa gtatgccggg
    11041 gcatcaccag gttgaatcgt ggccgttagc tgagcaatgg cgttggttaa caacctggct
    11101 ggtctggcgt cgtggtgcga aaacgcgtcc gcttgaggca tttattcaac tgctggatgc
    11161 gcctgactcg gcaaaacagg gttaccaatg agctattttt gatagttcat gcggctaata
    11221 tgctctatat agtgatgttc caatgactta tgactatatg gggcaaatat ggttacgcca
    11281 gtaagcatca gcaattacat atctcttcct gatgattttc ccgttcgtaa tattgcacct
    11341 caagtaaaag aagaacaggc ccccttcctg atcgtcatta aaatgtgatg ttaacaactc
    11401 tctgatgcgt gacagaacaa ccgtcagaga gatggaaatg cgatccagat cacaaatgca
    11461 ttgtattcac atcattaacc gttttaagat catttcatca ctttttcgca actcacccga
    11521 taatctgtta tgacaacaaa cactgtttcc cgcaaagtag cgtggctacg ggtcgttacg
    11581 ctggcagtcg ccgcctttat cttcaatacc accgaatttg tccctgttgg cctgctctct
    11641 gacattgcgc atagttttca catgcaaacc gctcaggtcg gcatcatgtt gacaatttat
    11701 gcatgggtag tggcgctaat gtcattgcct tttatgttaa tgaccagcca ggttgaacgc
    11761 cgcaaattac tgatctgcct gtttgtggtg tttattgcca gccacgtact gtcgttttta
    11821 tcgtggagtt ttaccgttct ggtgatcagt cgcattggtg tggcttttgc acatgcgatt
    11881 ttctggtcga ttacggcgtc tctggcgatc cgtatggctc cggccgggaa gcgagcacag
    11941 gcattgagtt taattgccac cggtacagca ctggcaatgg tattaggttt acctctcggg
    12001 cgcattgtgg ggcagtattt tggctggcga atgaccttct tcgcgattgg tatcggggcg
    12061 cttcttaccc ttttgtgcct gattaagtta cttcccttgc tgcccagtga gcattccggt
    12121 tcattgaaaa gcctcccgct attgttccgc cgcccggcat tgatgagcat ttatttgtta
    12181 actgtggtgg ttgtcaccgc ccattacacg gcatacagct atatcgagcc ttttgtacaa
    12241 aacattgcgg gattcagcgc caactttgcc acggcattac tgttattact cggtggtgcg
    12301 ggcattattg gcagcgtgat tttcggtaaa ctgggtaatc agtatgcgtc tgcgttggtg
    12361 agtacagcga ttgcgctatt gctggtgtgc ctggcactgc tgctacctgc ggcgaacagt
    12421 gaaatacacc tcggggtgct gagtattttc tgggggatcg cgatgatgat catcgggctt
    12481 ggtatgcagg ttaaagtgct ggcgctggca ccagatgcca ccgacgtcgc gatggcgcta
    12541 ttctccggga tatttaatat tggaatcggc gcgggtgcgt tggtaggtaa tcaggtgagt
    12601 ctgcactggt caatgtcgat gattggttat gtgggcgcgg tgcctgcttt tgctgcgtta
    12661 atatggtcaa tcatcatttt tcgccgctgg ccagtgacac tcgaagaaca gacgcaatag
    12721 ttgaaaggcc cattcgggcc ttttttaatg atacgtttta atgatttcca ggatgccgtt
    12781 aataataaac tgcacgccca tacataccag caggaatccc atcagacggg agatcgcttc
    12841 aataccaccc ttgcccacca gccgcataat tgcgccagag ctgcgtagac ttccccacaa
    12901 aataaccgcc accaggaaaa agatcagcgg cggcgcaacc atcagtaccc aatcagcgaa
    12961 ggttgaactc tgacgcactg tggaggccga gctaataatc atcgcaatgg ttcccggacc
    13021 ggcggtactt ggcattgcca gtggtacaaa agcaatattg gcactgggtt catcttccag
    13081 ctcttccgac ttgcttttca cttccggtga atcaatcgct ttctgttgcg gaaagagcat
    13141 ccgaaaaccg ataaacgcga cgatcaaacc gcctgcaatt cgcagaccgg gaatcgaaat
    13201 gccaaatgta tccatcacca gttgcccggc gtaatacgcc accatcatga tggcaaatac
    13261 gtacaccgag gccatcaacg actgacgatt acgttcggca ctgttcatgt tgcctgccag
    13321 gccaagaaat aacgcgacag ttgttaatgg gttagctaac ggcagtaaca ccaccagccc
    13381 caggcctatt gctttaaaca aatctaacat tggtggttgt tatcctgtgt atctggttta
    13441 tcagcgaaaa gtataagggg taaacaagga taaagtgtca ctctttagct aaccttgcat
    13501 cgcattgacc aaaacttgaa ccgatttagc aaaacgtggc atcggtcaat tcattcattt
    13561 gacttatact tgcctgggca atattatccc ctgcaactaa ttacttgcca gggcaactaa
    13621 tgtgaaaagt accagcgatc tgttcaatga aattattcca ttgggtcgtt taatccatat
    13681 ggttaatcag aagaaagatc gcctgcttaa cgagtatctg tctccgctgg atattaccgc
    13741 ggcacagttt aaggtgctct gctctatccg ctgcgcggcg tgtattactc cggttgaact
    13801 gaaaaaagtg ttgtcggtcg acctgggggc actgacccgt atgctggatc gcctggtctg
    13861 taaaggctgg gtggaaaggt tgccgaaccc gaatgataag cgcggcgtac tggtaaaact
    13921 taccaccagc ggcgcggcaa tatgtgaaca atgccatcaa ttagttggcc aggacctgca
    13981 tcaagaatta acaaaaaacc tgacggcgga cgaagtggca acacttgagc atttgcttaa
    14041 gaaagtcctg ccgtaaacga aaaaagaggt atgacgatgt ccagacgcaa tactgacgct
    14101 attaccattc atagcatttt ggactggatc gaggacaacc tggaatcgcc actgtcactg
    14161 gagaaagtgt cagagcgttc gggttactcc aaatggcacc tgcaacggat gtttaaaaaa
    14221 gaaaccggtc attcattagg ccaatacatc cgtagccgta agatgacgga aatcgcgcaa
    14281 aagctgaagg aaagtaacga gccgatactc tatctggcag aacgatatgg cttcgagtcc
    14341 caacaaactc tgacccgaac cttcaaaaat tactttgatg ttccgccgca taaataccgg
    14401 atgaccaata tgcagggtga atcgcgcttt ttacatccat taaatcatta caacaactag
    14461 ttgataacgt gacaacgtca ctgaggcaat catgaaacca cttttatccg caatagcaac
    14521 tgcgcttatt ctcttttctg cgcagggcgt tgcggaacaa accacgcagc cggttgttac
    14581 ttcctgtggc aatgtcgtgg ttgttccccc atcgcaggaa caaccaccgt ttgatttaaa
    14641 tcacatgggt actggcagtg ataagtcgga tgcgctcggc gtgccctatt ataatcaaca
    14701 cgctatgtag tttgttctgg ccccgacatc tcggggctta ttaacttccc acttttaccg
    14761 ctttacgcca ccgcaagcca aatacattga tatacagccc ggtcataatg agcaccgcac
    14821 cgaaaaattg cagacccgtt aagcgttcat ccagcaatag tgccgcactt gccagtccaa
    14881 ctacaggcac cagtaacgat aacggtgcaa cccgccaggt ttcatagcgt ccaagcagtg
    14941 ttccccagat cccataacca acaattgtcg ctacaaatgc cagatacatc agagacaaga
    15001 tggtggtcat atcgatagta accagactgt gaatcatggt tgcggaacca tcgagaatca
    15061 gcgaggcagc aaagaaggga atgattggga ttaatgcgct ccagattacc agcgacatca
    15121 ccgccggacg cgttgcgtgc gacatgatct ttttattgaa gatgttgcca cacgcccaac
    15181 taaatgctgc cgccagggtc aacataaagc cgagcatcgc cacatgctga ccgttcagac
    15241 tatcttcgat taacaccagt acgccaaaaa tcgctaaggc gatccccgtc aattgtttgc
    15301 catgcagtcg ctcaccgaaa gtaaacgcgc caagcacgat agtaaaaaac gcctgtgcct
    15361 gtaacaccag cgaagccagt ccagcaggca taccaaagtt aatggcgcag aacagaaaag
    15421 caaactgcgc aaaactgatg gttaatccat accccagcag caaattcagt ggtactttcg
    15481 gtcgtgcgac aaaaaagatg gccgggaaag caaccagcat aaagcgcaaa ccggccagca
    15541 tcagcggtgg catgttatga agccccactt tgatgaccac aaaatttagc ccccatacaa
    15601 ccactaccag tagcgccaac accccatctt ttcgcgacat tctaccgcct ctgaatttca
    15661 tcttttgtaa gcaatcaact tagctgaatt tacttttctt taacagttga ttcgttagtc
    15721 accgtttacg acggcattaa tgcgcaaata agtcgctata cttcggattt ttgccatgct
    15781 attcctttac atctctaaaa caaaacataa cgaaacgcac tgccggacag acaaatgaac
    15841 ttatccctac gacgctctac cagcgccctt cttgcctcgt cgttgttatt aaccatcgga
    15901 cgcggcgcta cgctgccgtt tatgaccatt tacttgagtc gccagtacag cctgagtgtc
    15961 gatctaatcg gttatgcgat gacaattgcg ctcactattg gcgtcgtttt tagcctcggt
    16021 tttggtatcc tggcggataa gttcgacaag aaacgctata tgttactggc aattaccgcc
    16081 ttcgctagcg gttttattgc cattccttta gtgaataacg tgacgctggt ggtgctcttt
    16141 tttgccctta ttaactgcgc ctactccgtt tttgctaccg tgctgaaagc ctggtttgcc
    16201 gacaatcttt cgtccaccag caaaacgaag attttctcaa tcaactacac catgctcaac
    16261 attggctgga ccatcggtcc gccactcggc acgctgttgg tgatgcagag catcaatctg
    16321 cccttctggc tggcagctat ctgttccgcg tttcccatgc ttttcattca aatttgggta
    16381 aagcgcagcg agaaaatcat cgccacggaa acaggcagtg tctggtcgcc gaaagtttta
    16441 ttacaagata aagcactgtt gtggtttacc tgctctggct ttctggcttc ttttgttagc
    16501 ggcgcatttg cttcatgcat ttcacaatat gtgatggtga ttgctgatgg tgattttgcc
    16561 gaaaaggtgg tcgcggttgt tcttccggtg aatgctgcca tggtagttac gttgcaatat
    16621 tccgtaggcc gccgccttaa cccggctaac atccgcgcgc tgatgacagc aggcaccttc
    16681 tgtttcgtca tcgggctggt cggttttatt ttttccggca acagcctgct aatgtggggt
    16741 atgtcagctg cggtatttac tgtcggtgaa atcatttatg cgccgggcga gtatatgttg
    16801 attgaccata ttgcgccgcc aggaatgaaa gccagctatt tttccgccca gtctttaggc
    16861 tggcttggtg ctgcgattaa cccattagtg agtggcatag tacttaccag tctgccgcct
    16921 ttctcgctgt ttatcatctt agcgttggtg atcgttgttg cgtgggtgct gatgttaaaa
    16981 ggaattcgtg caagaccgtg ggggcagccc gcgctttgtt gatttaagtc gaacacaata
    17041 agaatttaat tcagccttcg tttaggttac ctctgctaat atctttctca ttgagatgaa
    17101 aattaaggta agcgaggaaa cacaccacac cataaacgga ggcaaataat gctgggtaat
    17161 atgaatgttt ttatggccgt actgggaata attttatttt ctgggtttct ggccgcgtat
    17221 ttcagccaca aatgggatga ctaatgaacg gagataatcc ctcacctaac cggccccttg
    17281 ttacagttgt gtacaagggg cctgattttt atgacggcga aaaaaaaccg ccagtaaacc
    17341 ggcggtgaat gcttgcatgg atagatttgt gttttgcttt tacgctaaca ggcattttcc
    17401 tgcactgata acggatcgtt gacacagtag catcagtttt ctcaatgaat gttaaacgga
    17461 gcttaaactc ggttaatcac attttgttcg tcaataaaca tgcagcgatt ccttccggtt
    17521 tgcttaccct catacattgc cctgtccgct cttccaatga ccacatccag aggctcttca
    17581 ggaaatgcac gactcacacc agcagtcacg gtaatgttga tatgcccttc agaatgtgtg
    17641 atggcatggt tatcgactaa ctggcaaatt ctgacacctg cacgacatgc ttcttcatca
    17701 ttagtcgctt tgacaataat gataaattct tcgcccccgt agcgataaac cgtttcgtaa
    17761 tcgcgcgtcc aactggctaa gtaagttgcg agggtgcgta atactacatc gccgattaaa
    17821 tgcccgtagg tatcattaac caatttaaat cggtctatat ccaacaacat taaataaaga
    17881 ttcagaggtt cagcattgcg taactgatga tcgaaggatt catcaagaac ccgacgaccc
    17941 ggcaatcccg tcagaacatc catattgcta cggaccgtaa gcaaataaat tttgtaatcg
    18001 gttaatgccg cagtaaaaga aagcaaccct tcctgaaaag cgtcgaaatg cgcgtcctgc
    18061 cagtgatttt caacaatagc cagcattaat tcccgaccac agttatgcat atgttgatgg
    18121 gcagaatcca ttagccgaac gtaaggtaat tcatcgttat cgagtggccc cagatgatca
    18181 atccaccgac caaactggca cagtccataa gaatggttat ccgttatttc tggcttactg
    18241 gcatctctcg cgaccacgct gtgaaacata cttaccagcc actggtagtg ggcatcgata
    18301 gccttattga gatttaacaa gatggcatca atttccgttg tcttcttgat cattgccact
    18361 cctttttcac agttccttgt gcgcgctatt ctaacgagag aaaagcaaaa ttacgtcaat
    18421 attttcatag aaatccgaag t
//
LOCUS       DACADF010000089        18192 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.090, whole genome shotgun sequence.
ACCESSION   DACADF010000089 DACADF010000000
VERSION     DACADF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.090"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            56..1276
                     /locus_tag="ISB30_003013"
     CDS             56..1276
                     /locus_tag="ISB30_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAP1990621.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKSITVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRKEWGILCNND"
     gene            1249..1878
                     /locus_tag="ISB30_003014"
     CDS             1249..1878
                     /locus_tag="ISB30_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP1990622.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(1879..3039)
                     /gene="ybdL"
                     /locus_tag="ISB30_003015"
     CDS             complement(1879..3039)
                     /gene="ybdL"
                     /locus_tag="ISB30_003015"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HAP1990623.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTKMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTMDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3148..4236
                     /gene="hcxA"
                     /locus_tag="ISB30_003016"
     CDS             3148..4236
                     /gene="hcxA"
                     /locus_tag="ISB30_003016"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HAP1990624.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4246..4443)
                     /locus_tag="ISB30_003017"
     CDS             complement(4246..4443)
                     /locus_tag="ISB30_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAP1990625.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4556..6661)
                     /gene="cstA"
                     /locus_tag="ISB30_003018"
     CDS             complement(4556..6661)
                     /gene="cstA"
                     /locus_tag="ISB30_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HAP1990626.1"
                     /translation="MNKSGKYLVWTVLAVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGEVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6842..7255)
                     /gene="entH"
                     /locus_tag="ISB30_003019"
     CDS             complement(6842..7255)
                     /gene="entH"
                     /locus_tag="ISB30_003019"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HAP1990627.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7258..8004)
                     /gene="dhbA"
                     /locus_tag="ISB30_003020"
     CDS             complement(7258..8004)
                     /gene="dhbA"
                     /locus_tag="ISB30_003020"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HAP1990628.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFSQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIACPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8004..8861)
                     /gene="entB"
                     /locus_tag="ISB30_003021"
     CDS             complement(8004..8861)
                     /gene="entB"
                     /locus_tag="ISB30_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HAP1990629.1"
                     /translation="MAIPKLQAYALPEPHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8875..10485)
                     /gene="entE"
                     /locus_tag="ISB30_003022"
     CDS             complement(8875..10485)
                     /gene="entE"
                     /locus_tag="ISB30_003022"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HAP1990630.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GEWQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(10495..11670)
                     /gene="entC"
                     /locus_tag="ISB30_003023"
     CDS             complement(10495..11670)
                     /gene="entC"
                     /locus_tag="ISB30_003023"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HAP1990631.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHLAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11859..12815
                     /gene="fepB"
                     /locus_tag="ISB30_003024"
     CDS             11859..12815
                     /gene="fepB"
                     /locus_tag="ISB30_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP1990632.1"
                     /translation="MRLASLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIALFDKQLAAAKEQIKLPPQPVTALVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12819..14069)
                     /gene="entS"
                     /locus_tag="ISB30_003025"
     CDS             complement(12819..14069)
                     /gene="entS"
                     /locus_tag="ISB30_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HAP1990633.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLSVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMIGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDN"
     gene            14180..15184
                     /gene="fepD"
                     /locus_tag="ISB30_003026"
     CDS             14180..15184
                     /gene="fepD"
                     /locus_tag="ISB30_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HAP1990634.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLMMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15181..16173
                     /gene="fepG"
                     /locus_tag="ISB30_003027"
     CDS             15181..16173
                     /gene="fepG"
                     /locus_tag="ISB30_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HAP1990635.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16170..16985
                     /gene="fepC"
                     /locus_tag="ISB30_003028"
     CDS             16170..16985
                     /gene="fepC"
                     /locus_tag="ISB30_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HAP1990636.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(16982..18115)
                     /gene="fepE"
                     /locus_tag="ISB30_003029"
     CDS             complement(16982..18115)
                     /gene="fepE"
                     /locus_tag="ISB30_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAP1990637.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDPLDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNVNDVNFTPFKYQLRPSLPVKKD
                     GQGKAIIVILSALVGGMVACGGVLLRHAMASRKQDAMMADHLV"
BASE COUNT         4328 a         5142 c         4652 g         4070 t
ORIGIN      
        1 taattagcgg attcatctca cgagtcaccc cgtaacgttc agagagcgtt tttagatgtc
       61 tatttataaa atcccgcttc cactgaatat tctggaagct gcaaaagaac gtattacctg
      121 gacacttaat accctacccc gtatctgtgt ttctttttcg ggtggtaaag attccggatt
      181 aatgctgcat ctgacagctg aaattgcccg acaaatgggc aaaaaaatct gcgttttgtt
      241 tatcgactgg gaagcgcagt tctcatgcac tattaactat gttcagtcat tacgtgagtt
      301 ttacgccgat gtcatcgaag agttttactg ggttgcgctc ccgcttacga cgcaaaattc
      361 cctttctcaa taccaacccg aatggcagtg ctgggaacct gatgtcgaat gggtgcgtca
      421 gcccccgcaa gatgcgataa ccgatcctga ctttttctcc ttttaccagc ctggcatgac
      481 cttcgaacaa tttgtgcgtg agtttgctga atggttttca caaaaacgtc cggcggcgat
      541 gatgatcggc atccgtgcag atgagtccta caaccgtttt gtcgccatcg ccagtttaaa
      601 taaacaacgt tttgccgatg ataagccctg gaccaccgcc gcaccaggcg gtcatagctg
      661 gtacatttac cccatctacg actggaaagt ggctgatatc tggacctggt atgctaacca
      721 tcaacagctc tgtaacccac tgtataatat aatgtatcag gcaggcgtcc ctctgcgtca
      781 tatgcgaatt tgcgaacctt ttggcccgga gcaacggcaa ggattatggc tctatcacgt
      841 tatcgaaccg gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa aaagtggcgg
      901 catttacgcc ggacatgata atcattttta tggtcatcgg aaaatcctca agccagaaca
      961 tttagactgg caagaatatg cattattgct gctcaatagc atgccggaaa aaacagcgga
     1021 gcattaccgc aataaaatcg ccatttattt gcactggtat cagaaaaaaa gcatcacggt
     1081 tccacaaact cagcaagggg acattggggc gaaagatatc ccctcctggc ggcgaatatg
     1141 caaagtttta ctcaataacg attactggtg tcgggcatta tcatttagcc ctacgaaagc
     1201 gaagaactat cagcgttata acgaacggat aaaaggaaaa cgtaaggaat gggggatact
     1261 atgcaacaac gattaacgca ggatttaacc caatttctcg ccagcttgcc ggaagatgat
     1321 cgcatcaacg cgattaatga aatccgcatg gcgatccatc aggttagccc ctttcgtgaa
     1381 gagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa tgattacaac
     1441 ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgaaaa aatccattga aatagatggt
     1501 tttacccaac ccattgtggt cacgcatacg gataaaaatg ccctggaaat cgtggatggt
     1561 tttcaccgcc atgaaattgg taaaggctca tcatcgttaa aattacgctt aaaaggttat
     1621 ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac tattcgtcat
     1681 aatcgcgctc gtggacgtca ccaaatcact gccatgtcag aaatcgtccg agaactcagc
     1741 cagttgggat gggacgataa taaaattggc aaagagctgg gaatggacag cgacgaagtg
     1801 ttacgcctga agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca attttcccgc
     1861 gcctggacgg ttaaataact acagctggcg caggcgttct gctgctgcca gcaacgtcga
     1921 ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg cgcaaaacac
     1981 cgacagcgga atcgccgcta cgccgtgctc ccgcgtcagc cactggcaaa actcaacatc
     2041 atccatcgta gatacggcgc tgtaatccac cagcaaaaag tatgtacctt cgcacggtaa
     2101 aatctccagc cgactttcat ttaaggcatt caccagaata tcgcgcttct ggcgataaaa
     2161 gtccggtaat gcaagataat gctcaggttc tgcacgtagc atatcggcaa gcgccagttg
     2221 tgccggggta ttcaccgaaa aggtcagata ctgatgcact ttgcggatct cggcgctgat
     2281 gggcgcaggc gcaacgcaat aacccacttt ccagccggtc atatgatagg tcttaccaaa
     2341 cgatgacacc gccaccgctc gctcacgcaa ttgcggatgc gccagcacgc tggcatggcc
     2401 ctgttgtgaa aaattgatgt gctcgtagac ttcatcgcta atgacaaaaa tctcgtgccc
     2461 ggcgatcgcc tgccacagag cggcgaaatc agtctgctgc cagacagttg cactggggtt
     2521 atgcggggtg ttgaggatca ccagtcgggt gcgctcgctt aacaatgcgg caaattcctg
     2581 ccagtcaacg cgaaaatgcg gcggttgcag tgcaatacgt ttcactattc cccccgaaag
     2641 tgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt cgtcgccatt
     2701 gcgcaccaat gcggttatcg ccgcgtataa cgcctccgtc gcccctgccg tcacggtgat
     2761 attgctgtcg acatccggtt gatagccata cagacgttcc gttttctgag caatcgcctc
     2821 gcgcaaggcc tgcacgccgg tcatgggtgc gtattggttt gccccctggg caacgtggta
     2881 cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt gcgaaaggtt
     2941 aatcgcctgg tgttgctgcg ccagcgcgct catcttggtg aaaatagtgg tgccaagttg
     3001 tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac ctgacgaagt
     3061 tgcgtgttga aggcactata acacgatgct agtatttggc aatcaagacg tttagatgtc
     3121 taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt ggtcgtcggc
     3181 ccggctaact acttttcaca tcccggaagt ttcaatcacc tgcacgattt tttcactgat
     3241 gaacaacttt ctcgcgcggt gtggatctac ggcgaacgcg ccattgctgc ggcacaaacc
     3301 aaactgccgc ctgcgtttga actgccaggg gtaaagcata ttttgtttcg tggtcattgc
     3361 agcgaaagcg acgtgcaaca actggcggcg gagtccggtg acgatcgcag cgtggtgatt
     3421 ggcgtcggcg gcggcgcact actcgacacc gcgaaagccc tcgcccgccg tctcggtctg
     3481 ccgtttgttg ccgttccgac gatcgccgct acctgcgccg cctggacacc gctctccgtc
     3541 tggtataacg atgccggaca agcgctgcat tatgagattt tcgacgacgc caattttatg
     3601 gtgctggtgg aaccggagat tatcctcaat gcgccacaag aatatctact ggcggggatc
     3661 ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc agaaacgttg
     3721 ccgctaaccg tgcggctggg aatcaataat gcgcaggcca ttcgcgacgt cttgttaaac
     3781 agtagcgaac aggcgcttgc cgatcagcaa aatcagcaat taacgcaatc attttgcgat
     3841 gtggtggatg cgattattgc tggtggtggg atggtcggtg gtctgggcga tcgttttacg
     3901 cgtgtggcgg cggcccatgc cgtgcataac ggtctgaccg ttttgccgca aaccgagaag
     3961 tttctccacg gcaccaaagt cgcctacgga attctggtgc aaagcgcctt gctgggtcag
     4021 gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc gactacgctg
     4081 gcggagctgg aagtggatat caataatcag gtggagatcg acaaaatgat tgcccacacc
     4141 ctacgtccgg tggaatccat tcattacctg ccagtcacgc tgacaccaga taccttgcgt
     4201 gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgta caaatttagc agcagcgcgc
     4261 gccgcctttt ccaccatagc gcgcgtcctg ccgctcacgg aaaaactcct cgtaggtcat
     4321 cggcgtttga tcgggatggt taacccgcat atgttcgaca tagttgtcgt aatcaggcat
     4381 accaatcatc agtttcgccg cctgacctaa atattttccg gcttttgcca gtgaatcaaa
     4441 catctaaacc tccggtaata caaatgccct cctgaattaa gagggcttaa ttgctatcaa
     4501 cacgctcggg ctgagagtaa ggggaacgtt cagcacatcc cccctcactc tgactttagt
     4561 gcgcgccttt cgcctgcgcc acgatctcct cgacattttc cggcatcggc tcatatggcg
     4621 tttctttcgc cgttggcttc ggctctttca atgccgccag tgccgtctta atcgagaaca
     4681 gcgccagaac caccacgacc accataaaga agatggttaa cccggcatca agacggttgt
     4741 tgaacaccag ttgcgccagt tgtgactcag tatactgcga tggaatattg ccgctgtcga
     4801 tcattgcctg gaacttgtta gcaatcgcca ggaatccgac tttcgcatcc gggctaaacg
     4861 ctttctgcca gcctgcggtc agggtacaaa tcagcagcca ggccgttggt accagcgcca
     4921 cccaggcgta acgttgacgt ttcattttga acagcaccac agcacaaagc atcagcgcca
     4981 tccctgccag catctggttg gcaataccaa acagcggcca caaggtgtta ataccgccca
     5041 gcggatcgac ctcgccctga tgcaggaagt agccccacgc cagcacgcac agcgccgttg
     5101 ccagcaggtt agcaggcagt gaatcggtcc gtttcaggcc cggagacacc acgcccagca
     5161 gatcctgcaa cataaagcgc gcagcacgcg tacccgcatc caccgccgtc agaataaaca
     5221 gtgcttcaaa cagaatggcg aagtgatacc agaacgccac atccatcatg ccgcccagcg
     5281 caccgtgcag aatgtaggcc atccccaccg ccagggtcgg cgcaccgccc gcacgggaaa
     5341 tgatcgactg ttcacccact tcgctggcaa tctggtttag cgtatccggg gtaatcgcaa
     5401 agccccagct actcaccacc tgcgcggcag aagcaaccac atccgccgtc cctgccggag
     5461 ccagcaccgc catcgggctg ttcatggcga agtacacacc cggatcgatg atacaggcgg
     5521 aaaccagtgc cataatcgcc acaaaggatt ccattaacat cccgccgtag ccgataaagc
     5581 acgcctgccc ttcgttcgcc agcatcttcg gcgtggtgcc ggaagagatc agcgcatgga
     5641 agccagacac cgcgccacag gcgatggtga taaacaggaa cgggaacagg ttaccggtcc
     5701 ataccgggcc agtgccatcg acaaatttag ttagcgcagg catggtcagc gtcgggcgca
     5761 taattaaaat gcccaccgcc agaccgacga tagtcccgat tttcaggaag gttgagaggt
     5821 agtcacgcgg agccagcagc aaccacaccg gcagcaccgc cgccacaaaa ccgtagccca
     5881 ccagcatcca ggtcagctgc acgccggtaa agtcaaagta cggtgcccag gtcgggcttt
     5941 ctgccaccca gccgccagag ataatggcga aaatcaggaa caccagacca attaccgaca
     6001 cttcgccaat acgccccgga cgcaaatagc gcaggtagat ccccataaac agcgccagcg
     6061 gaatggtgaa cgcaacggta tacgttcccc acgggctatg agtcagggct ttcaccacaa
     6121 tcatcgccag cacggcaagg ataatgacca tgatcataaa gcaggccacc agcgctatca
     6181 ccccggcggt tggtcccatc tcttctttga ccagctcacc cagcgagcga ccgtcacggc
     6241 gcgtagaaac aaacagcacc atgaaatcct gcaccgcacc ggcgagaacc accccggcga
     6301 gcagccagat catccctggc aggtaaccca tttgcgccgc cagtaccggc ccaaccaacg
     6361 gacctgctcc ggcaatggcc gcaaaatggt gaccgaacag cacttttttg tccgtcggca
     6421 catagtccag cccgtcgtta tggcgcaccg ctggcgtcat acgcgtcggg tcaaccgcca
     6481 gcacattttt ggcgatatac aggccataaa aacggtaggc gattagatag atacagaccg
     6541 acgccaccac aatccacagc gcgttgatct gttccccacg atttaaagca atgtatccca
     6601 gagcaaatgc tcccattaca gcgagcactg tccagacgag gtatttccct gatttgttca
     6661 tagttgttat ccgtgtgcgt gtccatagag atgttacatt ttgtttctat aacatctttg
     6721 tacaatttaa caacacagca accatgtaaa tcggaattga aaccaatatt taacttaaca
     6781 atgttaactc gatcactccg ttaaccgagt tttcctgcaa tctcatttaa ttctgtcggg
     6841 ctcatcccaa aactgccgta cccagccgac aggtgcagca gcgccgcccc tgttcatcga
     6901 aaacgacaat ttcccagctc tgattttgtc gtccaagatg cagcggctgg cagacaccgc
     6961 gcacctttcc ctcagacacc gggcgatgat gtgtggcgtt aagttccgtg cccaccacac
     7021 actgtccgtc gcgggtcatc ataaatccgg ccatcgatcc cagcgtttcc gccagcgccg
     7081 ccgacgcgcc gccatgcagc aggccaaacg gctgatgagt acgggtatca accggcattt
     7141 cggcttcgag tacatcatcg cccagacggg tatacacaat tcccagatgc gccaccattg
     7201 tgttatcgct ggtggcgttc agctcgtcga gcgttaaatg acgtttccag atcatgctta
     7261 tgcccccagc gttgagccgc catcgaccac aatatcctgt agggtaatat ggctggcgag
     7321 gtcagaggcg aggaacaaaa tcgtgttggc gatctcttgt ggacaggcga ttttccctag
     7381 cggaatgccg agtttaaact gctcgccaaa gccgcgaata cgctgctctt cggcgtcatc
     7441 gctcacccac agcgtgcgtt gcatatcggt gtcggtggag cccggcgaaa ccacattaca
     7501 gcgcacgccg ctacccgcca gttccagccc gacgctcaac gccaggcttt tcagcgccgc
     7561 tttcgacgcg ccataagcac tcataccaat acgcggcgtg tgtgcggcgt cggacgcaac
     7621 agtaacaatc gccccgcccc gctgacggcg aaactggttc atggtctgct gaaacaggtt
     7681 aaacgcaccg ccgacgttga ccgcaaaagt ctgctgccag tcctctttac tgagctgatc
     7741 ggtcgcgccc atgcgcaaaa ttcccgccgc attgaccagc acgtccagtc gctccgtttc
     7801 agctaacagt cgctgacata cctgcgccac ctgcgcagca tcggcaacat ccattacttc
     7861 ggtcgcaaaa ggatattgct cctgactaaa cgcttgatca aaacctgtaa ctttcgcccc
     7921 cgcctcaaca aatgccagcg ccgtggcgta gcctatacct ttccccgcgc cagttaccca
     7981 gacattttta ccgctgaaat ccattatttc acctcgcggg agagtagctt ccaccaggcg
     8041 tcgatggtcg ggtttttcgc cagcatgaca aagtcgatat cgccatgcac tttgcgccag
     8101 cgcgccgcca gcgccatcat gcgcaccgaa tccagaccgt agtcgatcag gttgtcgtca
     8161 tcgaagggtt catcggactc gtccagcaac ggcaggatca cctcacgcag cgccgctttg
     8221 ctggcgggga taggtgctgg cagcaattct tcggtcatca ccacccgacc agaacgtccg
     8281 gccacatatt tcagcgacat caaatgctcg tcacggctga aatcggccag cgcatccgcc
     8341 accataaacg gtttaatatc gcgcataaat gcgtccgttg cggtggtcat acagccaatg
     8401 tgggcatata ccccggtaat aatcagctgg ttacgtccac tctctttgag catttgctcc
     8461 agcggagaac gatgaaatgc gctgtagcgc catttcacca gcaccgtgtc gtcggcatct
     8521 ggcgtcaggc gatccaccac cttttgctgt tccggcgaac gggtcaggcc aggcccccac
     8581 atatcattca acagcgcccg atcttcatcg ctctgctctt tcggctgggc ggtgtaataa
     8641 accgggatat tgtgctgttt gcagtagtcg cgtagtgccg caatattcgc gatcacctgc
     8701 tccatcatcg ggcagttctc accccagaag ctgacaaaat agtcctgcat atcatggatt
     8761 aacaacgcgg cacgttgcgg ttcaaaggcc cagtcgactt tattctgcgg aatatcgtga
     8821 ggctccggca gtgcgtaagc ctgtaatttt ggaatagcca tcgtgttctc tccttcaggc
     8881 tgatgtgcgt gacgccagcc actgacgtaa ttgtttttta tcgactttcc cgaccgccgt
     8941 cagcggaagt gaatctacac actccacgcg atccggtaat ttaaattcgg caataccctg
     9001 ttcacgcagg aaacgacgca cctgcaccgc gcgcagcggc tcttttacca ccagataagc
     9061 acagcttttt tcgcccatca gctcatcttc catgctcacc agtgcggcgt agatcaccgc
     9121 cggatggcgc agcagcaggt tttcgatctc ttcggcagcg atcttctcgc cgccacggtt
     9181 gatctgatct ttctcgcgcc cctgcacagt gatgtaaccc tctggatcaa tagagatcag
     9241 atcgccggaa cagtaaaaac cgttggcatc aaaggcgctg gcattgtgct gcgggctttt
     9301 gtaatagcca cggaaagtgt acggcccgcg cgtcatcagg cgtccaactt ccccttgcgg
     9361 cagtggattt ccttcggcat cggcaaccca tacttcatcg tccgggcaca ttgggtaacc
     9421 ctgggtatgg ataattttct ccgcactatc atcaagacgg gtgtagttca ccagcccttc
     9481 cgccatgcca aacacctgct gcaactggca gccaatctca gcgggaatac gcgccgcaag
     9541 cgtggcagaa agacgtgcgc cgccgacctg taacagtttc agcgaggcaa gctgcgcccg
     9601 gctttcgcct tcagccagtg cctgcaacca caggctgact gctggcggca ccagcgcggt
     9661 gacgttcacc tgatgttttt caatcaatgg gaagcaaagc gtggcgctgg ggtcggcagc
     9721 cagaacgaca gtgccgccag cgagaaatac ccccaacgat cccggtgaac tcatggcgta
     9781 gttatgagcc gccgggatcg cgcacaggta gcgcgtctgt tgtgtgaact gacaaatctc
     9841 gacgctacga cgcacgctgt agtagtagtc gttatgagtg cgcgggatca gtttcggagt
     9901 gccggtggtg ccgccggaaa gctggaaata ggccacttca tcagcaggag atggcgtggc
     9961 agtaaaatcc tcagccggat ggttaatcgc atcctgcaag ttatgctcac cgctgtcgtt
    10021 gagcagctgc accacgcgaa tggaagaatg ctctgcaaca aatgtgttga ggaaatcatc
    10081 cccgctaaat agcgcatgtt ggcgatcggc aatcagtaac gccggttcaa tctggctggc
    10141 ataggcgttc agttcactac gctgatggct gaacagcgcc agcaccggcg caacgcccag
    10201 tttcagcagc gcgaaaaagg taatgtaaag ttcagcgacg ttacccagtt gtaccagcgc
    10261 ggtttcacca ggtttaatgc cctgacggcg caaactacag gcgaggttat ccgccgcctg
    10321 attcagctcc cggtaactca actgccactc gccgtcgatc accgcgatgc tgtcactcgc
    10381 agcgtggcga gtcagaatgt cggtcagtgg caaatcctgc cagtagcctt tttcccgata
    10441 gcgacgggca aactcttccg gccatcgggt gaatggaatg ctcatcctcg ctccttaatg
    10501 caatccaaaa acgttcaaca tggtagaaag tttgacgcct gtttcgcgcc actcacccaa
    10561 cggtgaggac gcaggcacaa tccccgctcc ggcaaacaga cgcacctgat tttcccgcag
    10621 cttcgcgcag cggatggtca ccacccattc accgttacct tcgctgtcac accaacccac
    10681 aatgccgcca aacagttcgc ggtcgaacgg ctccagttca gcaataacct gagtcgcggc
    10741 cagatgcggg aaaccgctca gcgcaggagt tggatgcagc agacaggcca gagtcagtgc
    10801 gttttcttgc gaattcgctt taccttcaaa gggagttgcg agatgccaca gcgtcggcgt
    10861 ggtaatcaat tgtggagagg agggaacgtg taactcacta ctgcgttcgc gcagtacctc
    10921 tttcatcgcc tgagtcacca gttcatgttc atggcgatct ttttctgacg ccagcagacg
    10981 attacccgct tcgcgatcga gcacttcatc tggctgacga cgcgcggaac cggctaacgg
    11041 aatggagcta aaacgctcgc cgtctttacg tagcagcagt tccgggctgg cccccagcag
    11101 aacgccacca tcagccaacg gaacatggaa gttgtaacta accgggtttt gcgcaatcaa
    11161 ccgttccagc aatacgccac tatcaatggc ggcgtcagtg gtgatatcaa tcaaccgtga
    11221 caacaccact ttgtcgacct gcggcgtggc ggtaagtgcg gcagcacggg caaccatctg
    11281 ttcaaacgtg gtttgttccg gaatggcctg acgttccacc acgttcagcg actggctgcg
    11341 ggtgaaacgg cgtgcggaag cttgtttttc ctgacgggag aacgactgcc aggattcggg
    11401 aatatacagc gacgaaggct gacgtggatc gaagggaatc gccccgacca tcaccggatt
    11461 tttgatgccc tgcgctttgg catcggcaaa cagcgcggcg agtttttgct ggaagggact
    11521 gtcgggtgaa tccccgttca cagcgggttc atcgaagcgg gcgaaacatc ctgacgtcgt
    11581 aaaactgcgg tacggcgaca taaagaaaaa gcgattgggc gcaagtgttg ccatggtctg
    11641 ctgtacttcc tcagccagtg acgtatccat atcatcctcc acaaaatgat aaaggcttta
    11701 ataatgatta tcatttatat tttcggtcgc taacctaaaa gcaaacgcgc actatgtcaa
    11761 ctcttgaggt aacgcaatct gcaactcggg gttgcatctg gttgtggtta caatgaaaat
    11821 gagaagcatt aacttcatta ataacaggaa gctgatttgt gagactcgcc tcgctctacc
    11881 gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgcagtt caggccgccg
    11941 actggccgcg tcagattact gacagccgtg gcactcatac cctggaaagc cagccgcagc
    12001 gtattgtatc caccagcgtc accctgactg gctcactgct ggcgattgat gctccggtga
    12061 tcgccagcgg cgcgaccaca ccaaataacc gcgtagcgga tgaccagggc tttttacgcc
    12121 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagtg
    12181 cagaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggcgatt
    12241 cggcgctggc gctgtacgat cagctttcta ccatcgcccc aacattaatc atcaattacg
    12301 acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg catgagaaac
    12361 aagcggcaga gcggattgca ctgtttgata agcaactggc agcggcgaaa gagcaaatca
    12421 aattaccgcc gcagccggtc actgcccttg tctataccgc cgctgcacac agtgccaatc
    12481 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    12541 agttgcccgc aggcttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc
    12601 ttggtggaga aaatctggct gcagggttaa atggcgagtc actattcctg ttcgccggtg
    12661 atcagaaaga cgccgatgct atttatgcta atccgctgct cgcgcacctg cctgcagtac
    12721 aaaacaagca ggtttatgcg ctgggaaccg agacattccg cctggattac tacagcgcca
    12781 tgcaagtgct ggaacgactt aaggcgctgt tttaagcatt aattgtcgga cgctgtcacc
    12841 tgcggcggcg tctggcgaaa acgccgcaac tccaccagca ccagcaacaa caacacgccg
    12901 ataatcaaca aaccaaaacc gctcgcgctt gcggaagcaa ccggtgtcat catcgctccc
    12961 agaccgccta acagcgccgc acctatggca tcgcccgtca cgttttgcgc cgtccacaaa
    13021 ccgttaatcc gccctaacat cgcttccggg gtttgcgttt gcagcattgt atattgcagc
    13081 aacgaactaa ccgcgctcag ccagccgaac agtgccagac aaaccacgcc taaaatccac
    13141 atcggcatca ggccaaacag accaatggcg aggaacgccc ccaatgtgga gagcaacatc
    13201 aacagacctg gccgtacgct atgcgccagt tttccactgg ttaacgcgcc aatagccgca
    13261 ccgagcggga ttgccgcata gagaaagcca atttgcgccg ccgacatctg ccagttgtca
    13321 gccagcgccg gatacagtac ccgcaccgcg ctcgccatcg tcaataaacc ccccagcaac
    13381 gcaatccctc ccaccagcgg gctggcgagt agaaaacgaa atcctgccag taatgatttc
    13441 aacggatgct cacgcggctg cggcggtggt ggcaacgccg gaaggcttaa caacggtagc
    13501 aaggtaataa acgtgcccgc cgccgccagc ccgtagttcc aggctacgcc tccggtcgcc
    13561 agcagtaaac caccaatcat gggcgaaatc accgacccca gacgcacagt caacatggtg
    13621 atcgccccgg cctgcattag gttttcacgt cctaccagtg caggtgtcgc cgccagtagc
    13681 gccgtaacgc caagtgatgc gaaaaaacca tcccataaac caagtaaata gattgccagc
    13741 aaagacggct ccggcagcag cgcattcagg cacagtccaa tgaagccaat gccacaggtg
    13801 ccgcgcgcca gcaaaatcac ttttttgcgc tcatagcgat ccgccagcac accaccgatc
    13861 atcagaccga caaacatagc accgccggtc agcgtcaccg aaagccccac ctgccaggta
    13921 gaatgtgtca tcatctggat ctgcaccggc accgcgacgc tgagcaaacc cagagacaca
    13981 attgagataa aacgagcgag gaatactgcg cgaaacgccg ggtgcgtttt taacaggctg
    14041 aggttaagca gccaggattg tttattcatt acaatgcctt gccatcaaat attttaaata
    14101 cctattctta ggctgcacat gctaacatat ccaaataaga tcgataacga taattaattt
    14161 cattatcatg gaagttcgta tgtctggttc tgttgccgtg acacgcgcca ttgccgtgcc
    14221 cggattgctg ttattactga ttatcgcgac ggcattaagc ctgctcattg gagcaaaatc
    14281 actccccgct tccgtagtgc tggaagccct ctccggcacc tgccagagcg ccgactgcac
    14341 catcgtgctc gacgcacgac tgccgcgcac ccttgccggt ttactggcag gcggtgcgct
    14401 tggccttgcc ggggcgttaa tgcaaaccct cacccgaaac ccacttgccg accccggctt
    14461 gcttggcgtg aatgccggag ccagctttgc cattgtgctg ggtgcggcgc tgtttggtta
    14521 ctcttccgcg caggaacaac tgatgatggc cttcgccggc gcgctggtgg cgtcattgat
    14581 tgttgccttt accggcagcc agggcggcgg gcagttaagt ccggtgcgtt taaccctggc
    14641 gggcgtggcg ctggcggcgg tgctggaagg actgaccagc ggcatcgccc tgcttaatcc
    14701 tgacgtctac gatcaactgc gtttctggca agccggttcg ctggatattc gcaatctaca
    14761 taccttaaaa gtggtgctga tcccggtgct taccgccgga gcaaccgcgc tattactgag
    14821 ccgcgcgctg aacagtttaa gccttggcag tgacaccgcg acggcgctgg gcagtcgcgt
    14881 agcgcgcaca cagttgattg gtctgctggc aattaccgtg ctttgtggta gtgcaacggc
    14941 ggtagttggc ccgattgcct ttattggcct gatgatgccg cacatggcgc gctggctggt
    15001 aggtgccgat catcgctggt cgctgcccgt cacgctactc gctacccctg ccctgctgct
    15061 gtttgccgat atcattgggc gggtgattgt tcccggcgaa ctgcgcgttt ctgtagtcag
    15121 tgcctttatt ggtgcaccag tgctgatctt cctcgtacga cgtaaaacgc gaggtggcgc
    15181 atgatttacg tctctcgccg attaattatc acctgtctac tgttgctaat agcttgtgta
    15241 atggcaggcg tgtggggatt acgcagcggt gccgtcacgc tggaaacctc gcaggtattc
    15301 gccgcgctga tgggcgatgc gccgcgcagt atgacgatgg tggtcaccga atggcgttta
    15361 ccacgcgtgc tgatggcgct gttgattggc gcggcgctgg gcgtcagcgg cgcgattttt
    15421 cagtcgctga tgcgtaaccc gctcggcagc cctgacgtaa tgggctttaa caccggggcg
    15481 tggagcggcg tgctggtggc gatggtgctg tttggtcagg atctgacggc tatcgcgctg
    15541 gcagcaatgg cggggggtat tgtcacctcg ctgctggtct ggctgctcgc ctggcgtaac
    15601 ggtatcgaca cctttcggtt gattattatc ggtatcggcg ttcgcgccat gctggtggcc
    15661 tttaatacct ggctgttgct gaaagcgtct ttagaaacgg cgctaacagc gggtttgtgg
    15721 aatgccggat cgctcaatgg cctgacgtgg gcaaaaacct caccttccgc acccatcatt
    15781 atattaatgt taattgccgc cgccttgctg gtgcggcgaa tgcgcttgct ggaaatgggc
    15841 gatgataccg cgtgtgcgct gggcgtcagc gtcgaacgtt cgcggctgtt aatgatgctg
    15901 gtcgccgtag tgcttaccgc agctgccact gccctggcgg ggccaatttc ctttattgct
    15961 ttagtcgcgc cacacattgc ccgccgcatc agtggcaccg ctcgctgggg gctgacccag
    16021 gcggcgctgt gcggggcact tttactgctg gcggccgatc tctgcgccca acaactgttt
    16081 atgccgtatc aacttccggt tggcgtcgtt accgtcagcc tcggcggtat ttaccttatc
    16141 gtcttgttaa ttcaggagtc tcgcaaaaaa tgaccgaatc agtagcccgt ttgcgtggcg
    16201 aacagttaac cctcggatat ggcaaatata ccgttgcgga aaatctgact gtagaaatac
    16261 ctgatggtca cttcacggca attatcgggc caaatggctg cggtaaatcc acgttactgc
    16321 gtaccttaag ccgcctgatg acgcctgctc acgggcatgt ctggctggat ggcgagcaca
    16381 ttcaacatta cgccagtaaa gaggttgcac gccggattgg tctgttggcg caaaatgcta
    16441 ccacaccggg cgatatcacc gtgcaggagc tggtggcgcg tgggcgttac ccgcatcaac
    16501 cgctgtttac ccgctggcgc aaagaagatg aagacgcggt aacaaaagcg atgcaggcca
    16561 cgggaataac tcatctggcg gatcaaagcg tggacaccct ttctggcgga caacgccagc
    16621 gagcgtggat cgcgatggtg ctggcccagg aaacggcaat tatgctgctc gacgaaccca
    16681 cgacctggct ggatatcagt catcagattg atctactgga actgttaagc gaactgaacc
    16741 gcgagaaagg ctataccctg gcggcggtgc tgcacgatct taatcaggcc tgtcgttacg
    16801 ccagccattt gattgcattg cgggaaggga aaattgttgc tcagggagcg ccgaaagaga
    16861 ttgtcactgc tgaactgatc gagcgcattt atggtctgcg ctgcatgata attgacgatc
    16921 cggttgccgg aacgccgctc gtggtgccgc tcggacgaac ggcaccttca accgcaaaga
    16981 tttaaactaa gtggtctgcc atcatggcat cctgttttct ggatgccatc gcatgacgca
    17041 acaacacacc accacaagcc accatcccgc cgaccaacgc agaaaggatc acgataatcg
    17101 ccttaccctg accgtctttt tttacgggta atgacggacg caactgatat ttaaacggtg
    17161 taaaattcac atcgttgaca tttgtttttg ttaattgctc gactagatac tgacgattac
    17221 gtaattcacc gttcaattcc gcaacgtcag tgactgcttt ttcaatttcc agtttgcgtt
    17281 caataccgtc tgcaccgaga gaaatagaga aatcggggtc atctttaacg gcctgaccat
    17341 tactgtatac gggcttttta attcctgccg cgttggcaat gtcaagtgaa taattgaggc
    17401 gctgaatgtt tgcatcaagt tgatttttcg ttttaatgcg atcctgagcc agtttttctt
    17461 tttcaaactg ggttttgatc tccagtttat tacggacgtt ttctatcgac tctttcacca
    17521 ccaacgcaga gatataatcg atatacccgg ccaacacttt ctgtgcctct tcactggtgg
    17581 gtgcggtgaa acttagcgtc caggaagtat acaatgcaga ctcatctttt ttcttactgg
    17641 cattgtcatc aaccgctttc attttttcgc tcaatgcaac aatggcgcga tgcagatcca
    17701 gtgggtcgat tttcgcttct tttaattgat ccatcacata aggtgatgaa cgcaggtact
    17761 cctcaagcaa gctaacagac tgaaatttct tgataaacag gttaaatgct tctgtacgat
    17821 caattttgat atccagatcc agcacacgaa gcttagtgaa tgttttctcc agctcttgcc
    17881 actgaacagg ttctggaggc gtgacaaccg ccgcgctagt ccatttttgc ggcaggatga
    17941 aagagatcag caagcctgcg caggcaaacg caaaaacgac cgccatgacc gttttttttg
    18001 cccgccataa aacctcgatt agattaagta aatcaatttc attattactg ggcgacgcca
    18061 gaggatactc gggaaaatga gcttcacttc cctgtttaat attcagtgat gacatgtagt
    18121 gaaacctgag gttttagtcc gtaaagcaca acgatgtaca tagcattcta acaggaactg
    18181 agatttatcc ga
//
LOCUS       DACADF010000090        18192 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.091, whole genome shotgun sequence.
ACCESSION   DACADF010000090 DACADF010000000
VERSION     DACADF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.091"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            34..963
                     /gene="pfkB"
                     /locus_tag="ISB30_003030"
     CDS             34..963
                     /gene="pfkB"
                     /locus_tag="ISB30_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAP1990638.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNHKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1064..1354
                     /gene="ghoS"
                     /locus_tag="ISB30_003031"
     CDS             1064..1354
                     /gene="ghoS"
                     /locus_tag="ISB30_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAP1990639.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTHSATGKDPEITITTWEEWNSNRK"
     gene            1460..2320
                     /locus_tag="ISB30_003032"
     CDS             1460..2320
                     /locus_tag="ISB30_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP1990640.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2361..2897)
                     /locus_tag="ISB30_003033"
     CDS             complement(2361..2897)
                     /locus_tag="ISB30_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990641.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3044..3712
                     /gene="hxpB"
                     /locus_tag="ISB30_003034"
     CDS             3044..3712
                     /gene="hxpB"
                     /locus_tag="ISB30_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAP1990642.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3876..4466
                     /locus_tag="ISB30_003035"
     CDS             3876..4466
                     /locus_tag="ISB30_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP1990643.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4599..5990
                     /locus_tag="ISB30_003036"
     CDS             4599..5990
                     /locus_tag="ISB30_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP1990644.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6037..6300)
                     /gene="cedA"
                     /locus_tag="ISB30_003037"
     CDS             complement(6037..6300)
                     /gene="cedA"
                     /locus_tag="ISB30_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAP1990645.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            6483..8744
                     /gene="katE"
                     /locus_tag="ISB30_003038"
     CDS             6483..8744
                     /gene="katE"
                     /locus_tag="ISB30_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAP1990646.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAVPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEHRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPNVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADRADGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            complement(8791..9549)
                     /gene="chbG"
                     /locus_tag="ISB30_003039"
     CDS             complement(8791..9549)
                     /gene="chbG"
                     /locus_tag="ISB30_003039"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAP1990647.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQSLSKDG
                     DLPANLRSSQGFSSAFYGEEISETLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(9562..10914)
                     /locus_tag="ISB30_003040"
     CDS             complement(9562..10914)
                     /locus_tag="ISB30_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP1990648.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(11017..11856)
                     /gene="chbR"
                     /locus_tag="ISB30_003041"
     CDS             complement(11017..11856)
                     /gene="chbR"
                     /locus_tag="ISB30_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HAP1990649.1"
                     /translation="MQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYY
                     EFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQ
                     HYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHY
                     REEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPM
                     QIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTE
                     FNQ"
     gene            complement(11867..12217)
                     /gene="chbA"
                     /locus_tag="ISB30_003042"
     CDS             complement(11867..12217)
                     /gene="chbA"
                     /locus_tag="ISB30_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAP1990650.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(12268..13626)
                     /gene="chbC"
                     /locus_tag="ISB30_003043"
     CDS             complement(12268..13626)
                     /gene="chbC"
                     /locus_tag="ISB30_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HAP1990651.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVHRNWVIKLPDSVPTSVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(13711..14031)
                     /gene="chbB"
                     /locus_tag="ISB30_003044"
     CDS             complement(13711..14031)
                     /gene="chbB"
                     /locus_tag="ISB30_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAP1990652.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(14330..14668)
                     /gene="osmE"
                     /locus_tag="ISB30_003045"
     CDS             complement(14330..14668)
                     /gene="osmE"
                     /locus_tag="ISB30_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAP1990653.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            14870..15697
                     /gene="nadE"
                     /locus_tag="ISB30_003046"
     CDS             14870..15697
                     /gene="nadE"
                     /locus_tag="ISB30_003046"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP1990654.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAIRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            15927..16814
                     /gene="cho"
                     /locus_tag="ISB30_003047"
     CDS             15927..16814
                     /gene="cho"
                     /locus_tag="ISB30_003047"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAP1990655.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRASNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQALERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLKEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNDQQAS"
     gene            complement(16774..17349)
                     /gene="ves"
                     /locus_tag="ISB30_003048"
     CDS             complement(16774..17349)
                     /gene="ves"
                     /locus_tag="ISB30_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAP1990656.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(17552..18037)
                     /gene="spy"
                     /locus_tag="ISB30_003049"
     CDS             complement(17552..18037)
                     /gene="spy"
                     /locus_tag="ISB30_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAP1990657.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         4650 a         4596 c         4304 g         4642 t
ORIGIN      
        1 tgccagactg aaatcagcct ataggaggaa atgatggtac gtatctatac gttgacactt
       61 gcgccctcac tcgatagcgc aacaattacc ccgcaaattt atcccgaagg aaaactgcgc
      121 tgtaccgcac cggtattcga acccggaggc ggcggcatca acgtcgcccg cgccattgcc
      181 catctgggag gcactgccac agcgatcttc ccggcgggtg gcgcgaccgg cgaacatctg
      241 gtttcactgt tggcggatga aaatgtcccc gtcgctactg tagaagccaa agactggacc
      301 cggcagaatt tacacgtaca tgtggaagca agcggtgagc agtatcgttt tgttatgcca
      361 ggcgcggcat taaatgaaga tgagtttcgc cagctcgaag agcaagttct ggaaattgaa
      421 tccggggcca tcctggtcat aagcggaagc ctgccgccag gtgtgaagct ggaaaaatta
      481 acccaactga tttccgctgc gcaaaaacaa gggatccgct gcatcgtcga cagttctggc
      541 gaagcgttaa gtgcagcact ggcgattggt aacatcgagt tggttaagcc taaccacaaa
      601 gaactcagtg cgctggtaaa tcgcgaactc acccagccag acgatgtccg caaagccgcg
      661 caggaaatcg ttaatagcgg caaggccaaa cgggttgtcg tttccctggg tccacaaggg
      721 gcgctgggcg ttgatagtga aaactgcatt caggtggtgc caccaccggt gaaaagccag
      781 agtaccgttg gcgctggtga cagcatggtc ggcgcgatga cacttaaact ggcagaaaat
      841 gcttctcttg aagagatggt ccgttttggc gtagctgcgg ggagtgcagc cacactcaac
      901 cagggaacac gcctgtgctc ccatgacgat acgcaaaaaa tttacgctta cctttcccgc
      961 taacaaaaac attcccccag cattggggga atcaccacca acctgtcggc aacgcgtttc
     1021 tccgactatg ctcaaaagtc atgtgataac aaaggggtga actatggcca gtggcgatct
     1081 tgtccgttat gtaataaccg tcatgttgca tgaggataca ttgactgaaa ttaacgagtt
     1141 gaataattac ctgactcgcg acggtttttt gctcaccatg acagatgatg atggcaatat
     1201 ccatgagctg gggactaaca cttttggact tatcagtacc caaagtgaag aagaaattag
     1261 agaactggtt tcggggctta cccacagtgc aaccggcaaa gatcctgaaa tcaccatcac
     1321 gacctgggag gaatggaata gcaacagaaa ataaatggtt tttgggcaat aatcagtctg
     1381 tggtgtgcgt tagctcgtgt ttttacaccg cattcttgcg ctaaccttat gatctggcag
     1441 acaacatggg agagacatca tgtggcaggc aatcagtcgt cttttgagcg agcagttagg
     1501 tgaaggcgaa atagaactgc gtaatgaact gcctggcgga gaagtccatg ccgcatggca
     1561 tttgcgctat gcaggacgtg actttttcgt caaatgtgat gaaagggaac tgcttcccgg
     1621 ttttaccgcc gaagccgacc aactggagtt actgtcgcgt agtaaaaccg tcaccgtgcc
     1681 taaggtttgg gcagttggcg ctgaccgtga ctacagtttt ctggtgatgg attatctccc
     1741 acctcgcccg ctggatgcgc atagtgcatt tattcttggt cagcaaattg cgcgtttaca
     1801 tcaatggagc gaccaaccac aatttggcct cgatttcgat aacgcgctct ccacaactcc
     1861 acagcccaac acctggcaac gtcgctggtc aacttttttt gctgaacaac ggattggctg
     1921 gcagctggaa ctggcggcag aaaaagggat cgctttcggc aatatcgatg ccatcgtcga
     1981 gcatattcag cagcgtcttg cctcgcatca accgcagcct tctctgttgc acggcgattt
     2041 atggtccggc aactgtgcgc tgggtccgga tggcccgtac attttcgacc cggcctgtta
     2101 ctggggtgac cgagagtgcg acctggcgat gttaccgctg catactgaac agccgccaca
     2161 aatctatgac ggctatcagt cagtatcccc gctacctgcc gatttccttg aacgtcaacc
     2221 ggtttaccaa ctctacacgc tgttaaatcg tgcaaggtta tttggcggtc agcatttggt
     2281 tattgctcag cagtcactgg atagattatt agcagcatga tatgggttga ggataatggc
     2341 cgctccgtgc ggccttttga ttaaataaac ccaagcagag aaaagaacac ataacctgca
     2401 gcaatacaaa taaccggcag gatatacagc gagaaaaact gcaggaaaat agtatggtgc
     2461 ggaacaacaa tacgtgactc aatttgttca cgcgttagcc cctcttcccc tttggctttt
     2521 tccagaatga gttgatcttc aacaccttcc cgcaagaagc gcgcctggcg actcatccgg
     2581 gctccggaat cctgcaacgc cagtccgaca aaaatcagga tgaaaatcac ccagaacata
     2641 acgttcacgc caccattaaa attgggcgtc ggagagttgt accagaacag attcaaaaaa
     2701 ggcgtattcg cctgcatcat atcgatcatg acatgagtaa agtcgagcat gaccgcatta
     2761 atgccttcct ggttttcctg ccgggtattc ataaatttca gcaaagaaat caatgtagag
     2821 atcaatgcag gtataaaaat cacccaaccc aaaatccttt tcaaaacagc aatgcgtcca
     2881 gcttgttgat acgtcatgag ttctccttga ttaagacgcg tcgtttcact tagtttacct
     2941 gtagatatct gttttcgccc attctttaaa ggcgatatga taggcgctta atcataagca
     3001 cggcttaata ccttacatat aatgctctaa aggagaggtt gtaatgtcaa ccccgcgtca
     3061 gattcttgct gcaatttttg atatggatgg attacttatc gactcagaac ctttatggga
     3121 tcgagccgaa ctggatgtga tggcaagcct gggggtggat atctcccgtc gtaacgagct
     3181 gccggacacc ttaggtttac gcatcgatat ggtggtcgat ctttggtacg cccggcaacc
     3241 atggaatggg ccaagccgtc aggaagtagt agaacaagtt attgcccgtg ccatttcact
     3301 ggttgaagag acacgtccat tattgccagg tgtgcgtgaa gccgttgcgt tatgcaaaga
     3361 acaaggttta ttggtggggc tggcctccgc gtcaccgctg catatgctgg aaaaagtatt
     3421 gaccatgttt gatttacgcg acagtttcga tgccctcgcc tcggctgaaa aactgcctta
     3481 cagcaagccg catccgcaag tatatcttga ctgcgcagca aaactggggg ttgaccctct
     3541 gacctgcgtg gcactggaag attcggtaaa tggcatgatc gcctctaaag cagcccgcat
     3601 gcgttccatc gtcgttcctg cgccagaagc gcaaaatgat ccacgttttg tattagcaaa
     3661 cgtcaaactc tcatcgctga cagaactcac cgcaaaagac cttctcggtt aatgaccagg
     3721 gggcagtgat cacactgccc ctggttcttt atctgaattt gcattcaaat gacggattaa
     3781 tcgccaattt aagagaaaga gttacaccgt caccacttcc gtgcactgta taaaaaccct
     3841 atactgtacg tatcgacagt ttagtgagtt ttatcatgac ggcggaaggt caccttctct
     3901 tttctattgc ttgtgcggta tttgccaaaa atgccgagct gacgcccgtg ctggcacagg
     3961 gggactggtg gcatattgtc ccttccgcaa tcctgacgtg tttgttaccg gacatcgatc
     4021 acccaaagtc gtttcttggg cagcgattaa agtggatatc aaaaccgatc gcccgcgctt
     4081 ttgggcatcg tggttttacc cacagcctgc tggcggtatt tgcgctgctg gcaacctttt
     4141 accttaaggt tcctgaaagc tggttcattc cggctgatgc gttgcagggt atggtgctgg
     4201 gttatttgag ccacatactt gccgatatgc tgacacccgc cggtgttccc ctgctctggc
     4261 catgccgctg gcgtttccgc ttgcctatcc tggttcccca aaagggcaac caactggaac
     4321 gttttatctg catggcatta tttgtctggt cggtatggat gccccattca ttacccgaaa
     4381 acagcgctgt tcgttggtca tcgcaaatga tcaatacctt acagatccag tttcatcggc
     4441 ttattaagca tcaggttgaa tactaaaaag gcaaaaatcg cctttctgga ataagcaatt
     4501 ccatttgaat ataagagcca gctcacagtt ctgttaatct tgcgccaaca ctatgactgc
     4561 tacgcagtga tagaaataat aagatcagga gaacggggat gaactttcca ttaattgcga
     4621 acatcgtggt gttcgttgta ctgctgtttg cgctggctca aacccgccac aaacagtgga
     4681 gtctggcgaa aaaagtgctg gtgggtcttg tgatgggtgt ggtttttggc cttgccctgc
     4741 ataccattta tggttctgac agccaggtac ttaaagattc tgtacagtgg tttaacattg
     4801 ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat gccgctagtc ttcgcctcta
     4861 ttctgagcgc ggttgcccgt ctgcataacg catctcagtt aggcaaaatc agttttctga
     4921 ccatcggtac gcttttgttt accacgctga ttgcagcgct ggttggtgtg ctggtcacca
     4981 acctgtttgg tttgacggct gaaggtctgg ttcagggtgg tgcagaaact gcacgtctga
     5041 acgccatcga aagtaactat gttggtaaag tctctgacct gagcgttccg cagctggtct
     5101 tgtcctttat cccgaaaaac ccgtttgccg atctgaccgg agccaatccg acgtcaatta
     5161 tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc gctgaaactg ctgaaggatg
     5221 atgcgccgaa aggtgaacgc gtcttaaccg ctatcgatac cctgcaaagc tgggtgatga
     5281 aactggttcg cctggtcatg cagttgaccc cttacggtgt tctggcgctg atgaccaaag
     5341 tggttgcagg ttctaacctg caagacatca tcaaactggg aagtttcgtt gtcgcgtcct
     5401 acctcggtct gctgattatg tttgcagtgc atggccttct gctgggcatt aatggcgtga
     5461 gcccgctgaa gtacttccgt aaggtatggc cagtactgac gtttgccttt accagccgtt
     5521 ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac gcgtcgtctg ggtgttcctg
     5581 aatccatcgc cagtttcgcc gcctctttcg gtgcgaccat tggtcagaac ggttgtgccg
     5641 gtttgtatcc ggcaatgctg gcggtgatgg ttgcgcctac ggtgggcatt aacccgctgg
     5701 acccgatgtg gattgcgacg ctggtcggta ttgtcaccgt tagttccgca ggcgttgccg
     5761 gtgtcggtgg tggtgcaact ttcgccgcac tgattgtgct gcctgcgatg ggcctgccgg
     5821 taacactggt ggcattgtta atctccgttg aaccgcttat cgacatgggc cgtacggcgc
     5881 taaacgttag tggctcgatg accgctggca cgctgaccag ccagtggctg aagcaaaccg
     5941 ataaagccat tctggatagc gaagacgacg ccgaactggc acaccgttaa tcctgatttg
     6001 ttacgttaaa cgcctgtcat cccgacaggt gtttttttat tcttcgacct cattttcctg
     6061 gcggatctga tttgcccaac gctgggcgga ttcaggcaca gtaaacgccg gtgagcgcag
     6121 aaatgactcc cccatcagta caaacgcaac atatttgcca cgcagcatcc agacatctcg
     6181 aaacgaatcc atctttatcg catgttctgg cggcgcgggt tccgtgcgtg ggacatagct
     6241 aataatctgg cggttttgct gacggagcgg tttcttcatt actggcttca ctaaacgcat
     6301 attaaaaatc agaaaaactg tagtttagcc gatttagccc ctgtacgtcc cgctttgcgt
     6361 gtatttcata acaccgtttc cagaatagtt tccgaagcgg gatctggctg gtggtctata
     6421 gttagagggt tttttgacca aaacagcggc cctttcagta ataatttaag gagacgagtt
     6481 caatgtcgca acataacgaa aagaacccac atcagcacca gtcaccacta cacgattcca
     6541 gcgaagcgaa accggggatg gactcactgg cacctgaaga cggatctcat cgtccagcgg
     6601 ctgaaccaac accgcctggt gcacaaccta ccgccccagg gagcctgaaa gcccctgata
     6661 cgcgtaacga aaaacttaat tctctggaag acgtacgcaa aggcagtgaa aattatgcgc
     6721 tgaccactaa tcagggcgtg cgcatcgccg acgatcaaaa ctcactgcgt gccggtagcc
     6781 gtggtccaac tctgctggaa gattttattc tgcgcgagaa aatcactcac tttgaccatg
     6841 agcgcatccc ggaacgtatt gttcatgcac gcggatcagc agctcacggt tatttccagc
     6901 catataaaag cttaagcgat atcaccaaag cggatttcct ctcagatacg aacaaaatca
     6961 ccccagtatt tgttaggttc tctaccgttc agggcggcgc tggctctgcc gataccgtgc
     7021 gtgatatccg tggctttgcc accaagttct atactgaaga gggtattttt gacctcgttg
     7081 gcaataacac gccaatcttc tttatccagg atgcgcataa attccccgat tttgttcatg
     7141 cggtaaaacc agaaccgcac tgggcagttc cacaagggca aagtgcccac gatactttct
     7201 gggattatgt ttctctgcaa cctgaaactc tgcacaacgt gatgtgggca atgtcggatc
     7261 gcggtatccc gcgcagttac cgcactatgg aaggcttcgg tattcatacc ttccgcctga
     7321 ttaatgccga agggaaggca acgttcgtac gtttccactg gaaaccactg gcaggtaaag
     7381 cctcactcgt ttgggatgaa gcacaaaaac taaccggacg tgacccggac ttccaccgcc
     7441 gcgagttgtg ggaagcgatt gaagcaggcg attttcctga atacgaactg ggcttccagt
     7501 tgattcctga agaagacgaa ttcaagttcg acttcgatct tctcgatcca accaaactta
     7561 tcccggaaga attggtgccc gttcagcgtg tcggcaaaat ggtgctcaat cgtaatccgg
     7621 ataacttctt tgctgaaaac gaacaggtgg ctttccatcc agggcatatt gtccccggtc
     7681 tggatttcac caacgacccg ttattgcagg ggcgtttgtt ctcttatacc gatacacaaa
     7741 tcagtcgtct tggtgggccg aatttccatg aaattccgat taaccgcccg acctgccctt
     7801 accataattt ccagcgtgac ggcatgcatc gtatggggat cgacactaac ccggcgaatt
     7861 acgaaccgaa ctcgatcaac gataactggc cgcgcgaaac accgccgggg ccaaaacgtg
     7921 gcggttttga atcataccag gaacgcgtgg aaggcaataa agttcgcgag cgcagtccat
     7981 cgtttggcga atattattcc catccgcgtc tgttctggct aagtcagacg ccattcgagc
     8041 atcgccatat tgtcgatggt ttcagttttg agttaagcaa agtagttcgg ccgtatattc
     8101 gtgagcgcgt tgttgaccag ctggcgcata ttgatctcac tctggcccag gcggtggcga
     8161 aaaatctcgg tatcgaactg actgacgacc agttgaatat caccccgcct ccgaacgtca
     8221 acggtctgaa aaaggatcca tccttaagtc tgtacgccat tcctgacggt gatgtgaaag
     8281 gtcgcgtggt agcaattttg cttaatgatg aagtgagatc ggcagacctt ctggccattc
     8341 tcaaggcgct gaaggccaaa ggcgttcatg ccaaactgct ctactcgcga atgggggaag
     8401 tgactgcgga tgacggtacg gtgttgccta tagctgctac ctttgccggt gcgccttcgc
     8461 tgacggtcga tgcagtcatt gtcccttgcg gcaatattgc ggatatcgct aacaacggcg
     8521 atgccaacta ctatctgatg gaagcctaca aacaccttaa accgattgcg ctggcaggag
     8581 acgcgcgcaa gtttaaagca acaatcaagg tcgctgacca gggtgaagaa gggattgcgg
     8641 aagctgaccg cgccgatggt agttttatgg atgaactgtt aacgctgatg acagcacacc
     8701 gcgtgtggtc acgcattcct aagattgaca aaatcccggc gtaaatatgg ataaggtcac
     8761 gccccctggg gagtgacctt accaccttac ttacacatca tgataactac ccaaccgata
     8821 accacgctca gcaatcgcat atttcaacga cgctgaagtc agcacatcca gttccgttaa
     8881 ccgagggaag caataagcac tctggcgaat cgtattatcg ataaacgccg ggtggcacat
     8941 cacttccagc gagcgctcgc cgcgatgact ggagtcatca agcacttgca ggaacagtgt
     9001 ctcgctaatc tcttcaccat aaaacgcgct actgaatccc tgcgaactgc gcagattagc
     9061 cggtaaatca ccatccttag aaagcgactg acggtcgatg cgcaacgcaa tcccctcttc
     9121 agccgcaaac tttgccacaa tcgggaaaat ctgcgggaac atatgcacat gatgatggct
     9181 atcaagatgc gtaggtttgc gtccaaatag ctcaatgaaa cgcaaatact gactggcgag
     9241 ctcctgagta atttcttcca gcggtaaagc atcttcttct gccaactgcc agatccattt
     9301 tcccagcaca ccatcgcggg ttaaccccgg catagctgtc agaggcttgc ccatagtaag
     9361 gacaaagtgc atccctatgg ccagactcgg ttcatcacgg ctcaactgca ccgcatggtc
     9421 aatagcctgc ccattcacca gcgccgtcgt cgacgtgaca atcccattgc gacaggcctc
     9481 gataatgccg tagttctgac ctttgcttaa gccaaaatca tcagcattaa caatcagtaa
     9541 gcgttccata atcagcctcg gttaatgtgc ttttttaagc tctgcgatgc agtcggcaaa
     9601 atttggcagc catttctcgt gcgccagaat catctcgcgt gccagcagct cagcatcgcg
     9661 atcggaatgc accaacggac taaggtttag cgccagtaag acatcgttaa attctccact
     9721 aagtgcggcg ttgctggcag caatctcgaa gcctttgatg gtgtgaatca gccccatcac
     9781 tttatcatcg aaatgcgtaa tgcgcggatg tggcgtcgcg ccatcgcgcc ccagcgtaca
     9841 ggtcatttct accgcccagt ctgccggaat attatcaata tgcccatgat gcgggatatt
     9901 aacgtaatgt tctgcttgct tgtcgttata gatagcgttg atcacttcgc acgcagcatc
     9961 agaataataa gccccaccgc gctgttccag ttctttcggc ttaactttca actccgggtt
    10021 tttatatagc tcaaaaagtt gtttctcgac tttctgtacg acctgcgctc gtgcgccgcc
    10081 tttgtagtat tcgcccattt caatagccag catctctttt tgcttgaagt aataaagcaa
    10141 ataagaacac ggcaacagat tcagagaacg aattaagcct tcgctaaatg gcagatcgaa
    10201 aatattttta acgccagatg cttttaactg cccggatgcc acaccatcaa gcaattcggc
    10261 aaagcgtgat ttgccattta ccagcacatc cttaatgaac accatatggt tgaggccgaa
    10321 cagatcgata gataaatcat cgctgtcttt cagcatcaga acatcgcgaa taaacatctt
    10381 catgccgatc ggaatattac acacgccgat aaagcgttta aatccggtat gacgataaac
    10441 ggcttcagtg accattcccg ccgggttagt gaagttaatc acccatgcat tcggacatag
    10501 ttcttcgaca tcttttacga tgtcaaaaat caccggaatg gtacgcagac ctttaaacag
    10561 accgcccgcg ccgttagttt cctgaccaag ataaccatga cttaatggaa tacgttcatc
    10621 cagttcccgc gccggtaatt ggccaacgcg cagttgggta gtaacgaaat cagcatcttt
    10681 caatgcttcg cggcgatcca gcgttttata aagcttcatc gggacgccag cgttatcaat
    10741 catccgttgg cagagatcaa aaataatatc cagtttcgct ttaccacctt cgacatccac
    10801 cagccataat tcgctgaccg gcaattcgtg ataacgctta ataaatcctt ccagtaactc
    10861 cggggtatag ctgctcccgc caccaatagt gacgactttt aatttctggc tcataatttc
    10921 tcccttcagt acagaatact gatatctggc atatctgccc ccggacataa ataatccagc
    10981 aacaggacag atatgtgaat tgtcaggtat aacgacttac tgattaaatt cagtcaattt
    11041 cttacgatag ctcttaggcg taaaggacgt taatttctta aacgttttaa taaacaagct
    11101 cgggctacta taaccggcct caaacgcaat atccgtcact gaatagttgg tcatttccag
    11161 ttgttttttg gcaaaattaa tacggatttc attaataatc tgcattggcg ttttaccata
    11221 atatcgttga gtcgctcgcg tcaaatattc ctgtgatttg gctgacaacg tcaccatatt
    11281 ctccagcgcc gattcactaa actgctcttt atcatgcatt ttttctaccg tacttttcag
    11341 ccactgcggt atatcatcaa tcacctgttc ttcgcggtaa tgacgtaaac ggttaatgac
    11401 ataaaaagta accatctcaa caaactcttc cagccctgtt tcgcggaaat tcaatgaaga
    11461 aatcactgtt tccacatagg tgagaaaagc gttattggtc cggtatacct gcgaagctac
    11521 aaagcaataa ggcaacaacg gcaggtaatg ctgctcaaaa aagcgtttac tgatcccaac
    11581 gttcaatata cgcgtggcac caaactcata aaaactttga tggtgcgaac ctaacggaat
    11641 aaaaacaaaa tcgccccgtt ccagtaacac gcgcttaccg ttaatctcct ggaaataacg
    11701 cccggttaat accagagtaa attcataata gtcgtgctga tgcagtccgc tgatactctc
    11761 agttttatta tagataaaca catggaagtt tttgccatta aacaactgct gttctcgggc
    11821 agtggcaatt tccggcgcgt taatcactgg ctgcatcatc gactccttat gccttcagtt
    11881 tttcatgaag ctcaattaat tcagtaatca gttcacgcgc aagcatggac gtcattaaat
    11941 gatcctgagc gtggaccagc accagactca ctttcatctt accttcgcca gcatcgcctt
    12001 caatcagttt cgtctgtacc agatgcgctt cattcaatgc catccgtgac tgatccatca
    12061 tcgcttttgc tgcggcaaaa tcgccctgct tcgcctgttt cagtgccgca tacgccaggc
    12121 tacgcgcttg tccggagttg atgataagcc ccatcaccac ttcttccagc tcttcagctt
    12181 ccgtttgcgt atcgggaata ttatcgagat ccatcataca tcgttcctct ctattcttac
    12241 cggcacgatt acccgtaccg gcatcgatta aaatttcagg gcgttagcga tatcttcttc
    12301 gctctcttct ttatcaatcg cgttttgtgc tttgttagcg accacaacaa agggcagata
    12361 aattaacgtt gcgataccaa ggttgaagag tgcgaccagc aatgcggcga cgctaccgtt
    12421 ggtgttaaag aaggctccca gaccggttgg catggtccac ggtgcaatat tggtcaccgg
    12481 tggaataatg cccatgtagt acgctgccag ggtgattgcc gccagaatcg gttgtaccag
    12541 tacaaacggg ataaacatca ccgggttcat gataattggc agaccaaaca gaatcggttc
    12601 gttaatctgg aagatgccag acggcagcgc cagttttgcc acctgacgat aatcagcacg
    12661 tcgagaggcg ataaagatag ccaggatcag gcctaaagtc gcaccactgc caccaaggaa
    12721 aataaaggag tccagcatcg gcttggccca gatatggaag gtcttaccgg ctgccagcgc
    12781 cgcttcgacg gaaccatatt gctgataggt cgcgatattt tccagcgccc aaggcgtcat
    12841 aatgccgttg tccagtgcgg tcagcgccag cgcgccatga ataccgaaga accagagcag
    12901 tggaacaaag atcacatagg cccagcctac cacgctaccc aacgatgcca gtggggttga
    12961 gatcgtatcc ataatgatct gatggaagtt ggtgccccag gtattcaacg cccaggcaat
    13021 aatccccatc acggaaagaa taataaaacc aggaattaat gctgagaaag agcgcgaaac
    13081 cgatgtcgga acgctatcag gtaacttaat gacccagtta cgatgaacaa taaaggtaaa
    13141 catttctgcc accaccaggc caataataat cccggagatg atattcgccc cacctaacca
    13201 gtttgcgcca accgcatagg cctcaccgac actatatggg gtgacggtca taaatgctgc
    13261 aacggataac aacccggccg ccagcgcatc gactttacgc tcttctgcca gcgccatgcc
    13321 aataaagaac ggtgccatta aagacattat ccctaatgtt ccgttatata cgttgccgcc
    13381 aatacctttc agaccattaa gtgtttcaat ggttgaggca tccaggcgaa tacctaagga
    13441 ataaaaaaac gacccctccc caaagcttag aaaaacgttg ttaattaata caaacatggc
    13501 ccccgcaagg gttaacggca ttaagcgaat aaagccattt ttgattgcat taacgtgtgg
    13561 ctgctttcct attttaactg caaaagggag gagtaccttt tcaagcgatg caataacatt
    13621 actcatagaa aaataccctt aaaaaccgca atttaaatat tgcggtattg atttatgaaa
    13681 taactctttg acgggaaaat ttaaaataaa ttaatttgct gcggcttttt taatcgctgc
    13741 aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca taaagcagcg agtcaattac
    13801 ttcaaccggt ttgttgggta acaaacgctg gatttcgggc aacatataag caatctgcgg
    13861 ccctaataac acgacatcgg cattctgacc tttttcacca gccagtgttt ccggaaatgc
    13921 ttcaataatg accggaactt catatttttc tgcctgtgcg cgcatttttg ataccagtaa
    13981 agaggtagac atgcccgcag aacaaaacag ataaatgtgt ttcttttcca taaaactgcc
    14041 ctcatcgacg attatctgtc agccagacac tccgcaagcc ttaacctgct tccatgctct
    14101 gggtaacttg cgaaaccaac atgacttttt ttgtggctga aaggagtata cggtataggc
    14161 ctgagcaata gtatttcttt gacctgataa attgtctctc cttgacctgt ggttatgacc
    14221 ctcttcacat ttcgggcaaa taattcgcga agataattaa gaacacaggc caaaaaaaac
    14281 cggcgcaatg gccggtttcc gttgttactc aagctttcag gcgaagtgat tacttcgtag
    14341 cctgcggatc agtgtcgtat tcagcacagg tctgataacc ggagttgatg acatgtccgg
    14401 tatcatctaa cgcgacaaag taggtttctg ctttaccatc acgttgaccc aggatgtagg
    14461 tctggcaagt accacgagca tggatcatgc tcacttcaga cgaaggttta cccgcaattt
    14521 gtgcaacctg cgcccggctc atgccttttt tgacgtcttt caccacaggc tgtacaaact
    14581 ggtctttggt acgatcataa gccgtacaac ccgccagcat ggttaatacc gccgctgcac
    14641 tcagaattcc tgccatattc ttgttcatat tccgtcctct tgtttatcag cgtgttagat
    14701 aagcctggaa tacattgggc gctttttcaa gcccgtgaac gaaacggctc cgctttcaga
    14761 ggattcctgt atgacgtttt aaccaccatt cagcccgctg tcgcttgtcg tttcagtagc
    14821 aacgggttag ctttaaggaa gttttgtctt ttctgtctgg aggggttcaa tgacattgca
    14881 acaacaaata ataaaggcgc tgggcgcaaa accgcagatt aatgctgaag aggaaattcg
    14941 tcgtagtatc gattttctga aaagctacct gcaaacttat ccgttcatta aatcactggt
    15001 gctcgggatc agcggcggtc aggactccac gcttgccggt aagctgtgcc agatggcgat
    15061 taatgagctg cgccaggaaa ccggcaacga atcactgcaa tttattgcca tacgcctgcc
    15121 ctatggtgtc caggctgacg aacaagattg ccaggatgcc attgccttta ttcaaccgga
    15181 tcgcgtatta accgttaata tcaagggcgc ggtactcgcc agcgaacagg cgctgcgcga
    15241 agcgggcatt gaactgagcg attttgtccg tggcaatgaa aaagcgcgtg agcggatgaa
    15301 agcacaatat agcattgcgg gtatgaccag tggtgtcgtg gtgggcaccg atcatgcagc
    15361 agaagctatt accggattct tcactaaata tggtgacggc ggtacggaca ttaatccgct
    15421 gtatcgtctc aacaaacgtc agggtaaaca gttactggcg gcattaggtt gcccggaaca
    15481 cctttataag aaagcgccaa cggccgatct tgaagatgat cgcccttctc taccagatga
    15541 agtggcactc ggcgtgacct atgacaatat cgacgactat ctggaaggga aaaacgtacc
    15601 tgaacaggtc gccagaacaa tagagaactg gtatctgaaa accgaacata aacgccgtcc
    15661 gccaattacc gttttcgatg atttctggaa aaagtaataa tttgcaccta ttggattgtg
    15721 cgaaaaagag taatttgttc acgccggatg cggcgtgaac gccttactcg acctacaaaa
    15781 ctatgcaaat tcaatacatt gcaggagtcg aataggcctg acaggcgtag cacgtcagac
    15841 ggtgtaacct ttgtcatcaa cccgcctctt ttttaatcgc tccccgcctg ttacactgga
    15901 tagataacca gcattcggag tcaacagtgg tacggcgttt aacttctccg cggctcgaat
    15961 ttgaagctgc ggcaatttat gaatatcccg aacatttacg ttcattcctt aatgacttac
    16021 ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga caccatgccg ctctatatcg
    16081 gcaaaagcgt taatatccgc agtcgggtgc tttctcattt acgcaccccg gatgaagccg
    16141 ccatgctgcg acaatcccga cgcatcagct ggatatgtac cgccggtgaa atcggcgctc
    16201 tactccttga agcgcgatta atcaaagagc agcagccgct gtttaataaa cgcttgcgcc
    16261 gcaatcgcca gctctgcgcc ctgcaattaa atgaaaagcg tgttgatgtg gtgtatgcca
    16321 aagaggtgga tttttcacga gcctccaacc tgtttggcct gtttgccaat aggcgcgcag
    16381 ctttgcaagc attgcagacc atcgccgatg aacaaaaact ttgttatggc ctgttgggac
    16441 tggaaccgtt aagccgcggt cgtgcatgtt ttcgttctgc attaaaacgt tgcgccgggg
    16501 cgtgttgcgg taaagagagc catgaggaac atgcgctacg cttgcgccag gctctggagc
    16561 gtttgcgggt ggtgtgttgg ccttggcaag gcgcggtggc attgaaagaa cagcacccgg
    16621 aaatgactca atatcatatt attcaaaact ggctgtggct gggggcggtt aattcactga
    16681 aagaagcgac aacgttaatt cgggcacccg ccgggtttga tcacgacggt tataaaattc
    16741 tttgtaagcc gctgctttcc ggtaactatg aaattactga acttgatccg gtgaatgacc
    16801 agcaagccag ttgatttcac tgaacaacag tttaccttgc ggttgcaaaa gccgcagggt
    16861 gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg agcagtttgt cgcccaattg
    16921 ccacgccccg tttattacaa acaccacccc gccgcgtgaa ccaaaggtgg taaacgtacg
    16981 ttcggcaatt ctgactttcg ccttacagac atccaggcgt gtcatgatgt tgaaatccat
    17041 cgacatctgc cctgccgtca gtttcgcttt taccaccaga tccgctgcaa aggaaaaagg
    17101 ttgtaacggt tttaaggtat ggttaaagcg gtctgcgctt tcaaggaaca tctcgccgcc
    17161 ttccagcaac gtcactatcc tttccatacc gggaaacaaa gaaaactccc cattcgccgc
    17221 gatggatgta atgctggcac gccagtaaaa atcacgtttt gccggtggga aagtgcaaat
    17281 ttcgcgcgtt tcaccggcag cgtttcgcca cagattcacc gacattttac gcatatcaaa
    17341 gtattccata cccgctcccg gcccgttctt ttcattgtta tcgttcaggt aaccgtggat
    17401 ggttatcggc aactcgggtg atttacgtta gtggcgatca ggcttttttt gcatctgttt
    17461 gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt agtggacaag
    17521 accggcggtc ttacgttttt tggctgaaag attattcagc agttgcaggc attttacctt
    17581 ttgccgctgg acgttctgtc agacgcttct caaaattagc attaaattgc tttttctgtt
    17641 ccggcgtcag gatgttgtaa atcttgttct gggtttccat gtgcgccagc atgttagctt
    17701 tgcgctgttc ttccattttt gcgatctgcg cttcagcttt tgctttatcg aaggtatcgc
    17761 tggcaatgat gtcatgcatt gcgcggcgtt cttccagtgg cggacgtttc atctggtcac
    17821 gctggccttt catgatttcg cggatctgct gtttctgcgc gtcggtcagg ttcaggtctt
    17881 tgaacatcat gtcctcatgc ggaccgaact tgcctttatg gtgcatcatc ggcttcgcgt
    17941 cagccggtgc tgcagtagtg gtgtctgcgg catgggccag gttagccgcg ccaagagcca
    18001 gggtagaggc aacaaacagt gcagttaatt tacgcatatt ctatatcctt cctttcagtt
    18061 atttattacg gctttcttaa gtagcgtgcc gtgttgacga gattaacttt actgacttta
    18121 gcgtcaatta atcagagcaa cggtaaaaca atgaaagtgt aaaaaacact tttgcgccaa
    18181 ttatggagaa aa
//
LOCUS       DACADF010000091        18099 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.092, whole genome shotgun sequence.
ACCESSION   DACADF010000091 DACADF010000000
VERSION     DACADF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.092"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            100..1086
                     /locus_tag="ISB30_003050"
     CDS             100..1086
                     /locus_tag="ISB30_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP1990658.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGEEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEADQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            1131..1634
                     /locus_tag="ISB30_003051"
     CDS             1131..1634
                     /locus_tag="ISB30_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP1990659.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            1634..2935
                     /locus_tag="ISB30_003052"
     CDS             1634..2935
                     /locus_tag="ISB30_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP1990660.1"
                     /translation="MDSYIALTLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRDLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLVILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLITLMMITYIPQISLFIPQLLGLM"
     gene            complement(2991..3998)
                     /locus_tag="ISB30_003053"
     CDS             complement(2991..3998)
                     /locus_tag="ISB30_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAP1990661.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVSIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(4115..5161)
                     /gene="ansB"
                     /locus_tag="ISB30_003054"
     CDS             complement(4115..5161)
                     /gene="ansB"
                     /locus_tag="ISB30_003054"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAP1990662.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(5337..6056)
                     /locus_tag="ISB30_003055"
     CDS             complement(5337..6056)
                     /locus_tag="ISB30_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAP1990663.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(6240..6566)
                     /locus_tag="ISB30_003056"
     CDS             complement(6240..6566)
                     /locus_tag="ISB30_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAP1990664.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLGEVRTSEL
                     FDVWWD"
     gene            complement(6566..7285)
                     /gene="trmB"
                     /locus_tag="ISB30_003057"
     CDS             complement(6566..7285)
                     /gene="trmB"
                     /locus_tag="ISB30_003057"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP1990665.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            7446..8498
                     /gene="mutY"
                     /locus_tag="ISB30_003058"
     CDS             7446..8498
                     /gene="mutY"
                     /locus_tag="ISB30_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP1990666.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIATANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8526..8801
                     /locus_tag="ISB30_003059"
     CDS             8526..8801
                     /locus_tag="ISB30_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAP1990667.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            8866..9945
                     /gene="mltC"
                     /locus_tag="ISB30_003060"
     CDS             8866..9945
                     /gene="mltC"
                     /locus_tag="ISB30_003060"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAP1990668.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            10147..11403
                     /locus_tag="ISB30_003061"
     CDS             10147..11403
                     /locus_tag="ISB30_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAP1990669.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAVLVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(11452..13587)
                     /locus_tag="ISB30_003062"
     CDS             complement(11452..13587)
                     /locus_tag="ISB30_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAP1990670.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALADTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHVVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVALGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFCVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDANGMKRLYGYVLK"
     gene            13980..14687
                     /locus_tag="ISB30_003063"
     CDS             13980..14687
                     /locus_tag="ISB30_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP1990671.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            14793..14868
                     /locus_tag="ISB30_003064"
     tRNA            14793..14868
                     /locus_tag="ISB30_003064"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14826..14828,aa:Phe,seq:gaa)
     gene            15066..16331
                     /locus_tag="ISB30_003065"
     CDS             15066..16331
                     /locus_tag="ISB30_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAP1990672.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVRAAEVKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSYRGS
                     KMRTPHLVPLSQQAVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKVNNPLKR"
     gene            16587..17630
                     /locus_tag="ISB30_003066"
     CDS             16587..17630
                     /locus_tag="ISB30_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP1990673.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAIGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLQRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYIWPWILPF
                     SAAGMKHTGIQYQ"
BASE COUNT         4493 a         4542 c         4296 g         4768 t
ORIGIN      
        1 agacaagttg tcagacaggt taaccaattt gtcgcacaaa gcactgataa gcacaattag
       61 atatcaacca atttccattc ttcaacaggg agttttttta tgaaaatgag gtcatttacc
      121 cgctcattag tctgcgcatc cttgctggct ctggttagca ctggcgtaaa cgccgcagaa
      181 aaagtgacgt taaagttagc acacaacctg gaaagaagtc atgttgtaca tcagagtttt
      241 gaagagctgg cgaaagaggt aaaacagctg tcaaaaggta atatggtgat ccgtatttat
      301 cccagcagcc agatgggtaa tgcgcgagaa actatggagt tgttgcaaaa tggtgcactg
      361 gatatgacca aaggttccgc cagtgatctg gagtcttttg ataatattta tgctatctat
      421 aatttgccgt tcttatttaa agatcaggcg cacttcaaca aagtggtttt tggtgaagtt
      481 ggtgaagaaa ttatggattc aacaaaagat aaaggctttt ttgctttatc agcttatgta
      541 gcgggtacac gcagtttcta tgcgaaaaaa ccgattacta agccagaaga tcttaaaggg
      601 ctgaaaattc gtgttcagcc aagcccgacc accatcaaaa tgattgaact gatgggtggc
      661 tcacccactc caatttcatt tggcgaagtt tatactgcga tgcagcaagg tgtagtagat
      721 ggcgcagaaa ataacgtgcc ttcctgggtg cagactagac atatcgaaat tgccaaagtc
      781 ttttctgaag atgaacatgc ctcaattcct gactttttgg ttatctccac caaaacatgg
      841 aataaattaa ccccagaaca gcaacaaatc ctcgaaacag cggcgaagaa atccgaagct
      901 gatcaacaaa aattgtggga aaaaattgac gctgacacac gtgctcaggc gaaagctatg
      961 ggtggtgaaa tcgttaaggt tgataaagcc ccattccgtg ccgcagttca acctctgttt
     1021 gacgacttca aaaaagatcc aaaacaagct gctttgttag agaaattcga caacgctgct
     1081 caataatatt tcgggccagt ctctggccct gattttccag gatgcttaca atgatattta
     1141 accgcctgaa acttgcggta gaccgcgtca ttgccgcgtt ttctgtcgcc gtaatgcttg
     1201 ctctggttgt atgcgtagtc tggcaagtat ttagccgcta cgtacttaac cagccaagta
     1261 cattaaccga tgagctggca cgctttttga tgatctgggt aggattatta ggtgctgcat
     1321 ataccgtcgg cgcgcagaga catctctcta tcgatttatt tgctctcgcc ctgaataagc
     1381 gcaagcaatt acttcttagt atagtcatta acgttcttat tttaggtttt gccggttcgg
     1441 ttattgtgac tggtggcctg aaattaattg ataaaacact ggcaacatca caggtatctg
     1501 ccgcgatgca aataccgatg ggatacgtat atatcattct gccattaagt ggattggtga
     1561 tgatgtttta tgccttgtgt tttattaatc aaagcattca acaactgaaa caacctgcgc
     1621 aggaggccag ttaatggata gttatatcgc gcttacactg tttggctgtt tttttgtgct
     1681 ggtctttatt ggtgtgccca tttcgttctc aatcggtatt gccactgtag cttcaatgct
     1741 gttgatgttt ccctgggata tcgcggcaat cactgtttcg cagcgactgg ctaatggact
     1801 ggataacttt gccctattgg caatcccctt tttcatcttt gccggaacgc tgatgaacag
     1861 cggcggtatt gcgatacgtt tgattaacct cgcccaggta atggttggcc gcgttcctgg
     1921 ttcgcttggt catgtgaacg tcctggcgaa tatgatgttt ggttccattt ctggttcagc
     1981 tgttgcggca gctgctgccg ttggtggcac actgaatccc attcagacca aagaaggtta
     2041 tgatccggca ttttctaccg ctgtaaacgt ctcatcttgt attactggat tactgatacc
     2101 gccctcaaac gtgttaattg tgttttccct gacagctggc ggcgtttctg tagcatcgct
     2161 atttatggcg ggatatttgc cagggatctt aatgggtctc gcggtaatga tagtctgcgg
     2221 cattattgct aaacgccgtg gctacccact ttcagaaaga gcaacctttg ctcaggcatg
     2281 taaagcgttt ctggatgcct taccaagttt gcttctggtg ttcattgtaa tgggcggcat
     2341 tctgggcggc atttttaccg caactgaagc gtcagcaatt gccgttgtct acacatttat
     2401 tctctcggtg ctgatttacc gggaagtaaa atggcgcgat ttacccaaac tgattctgga
     2461 atcggttgtt accacttcga ttgtattact gttgatcggt ttttccgtgg gcatgtcctg
     2521 ggcaatgacc aatgctgata ttccttatat gatcagcgac gcgttgatgg ggatttctga
     2581 taacccactg gtcatattgc tgctgattaa tatcgtctta ttaattgtcg gcatctttat
     2641 ggacatgaca ccggctgtac taatcttcac accaatcttc ttgccgattg cacaagagct
     2701 gggaatggat ccggtacatt tcggtattat gatggtggcc aacctttgta ttgggctgtt
     2761 aacaccgccg gtgggtagtg cgctatttgt tggttgttct atttcaggtg tgaagattca
     2821 acatttgatc aaaccattgc tgccatttta tgccgcattg ttaatcacgt tgatgatgat
     2881 cacttacatc ccgcaaatat ccttgttcat tccacagcta ctagggctaa tgtaaaaaat
     2941 aatttgccgg ataacttttt atattatccg gcaaaggtac ttaacatgag ttacttaatc
     3001 actccattgc gccgcaattg ttcggtatct tttttccagc gttcattagc ctgattgata
     3061 tcgtcaattt tcatcaaaat cgcgactttt ttactgttta tttctgaaac tttcatcgtt
     3121 aacggatatt gccctttctc gagcgttatc cagtcatcct gcaatttacg gctaagtttt
     3181 tgtgattgca gaaaagtctg tcgccgttgt tcgttgatat cgaaatctcg cttctgctga
     3241 atcgacactt cataacactc ttcactctgt ttatgacatg ccgcaatcgc cggttgataa
     3301 acctctttgt catatttaac cgcgaacgcc tcgctatcat tctgtttatt aaactcatca
     3361 cgctgttggt gaattttttt aaaggcgtct tcgcgggtca tttgcttacc gtttgcatct
     3421 tttccccaat atgcatccgc tgctttctgc gccgccacca tttgcttatc aagcgctttc
     3481 acttcctttt ccagcgcagg aatcgagttc ttctggttat tcaacgcaga ctcaagctga
     3541 ctaaagcgat aattgaaatc ttcgctattc acgatcagat atttattatt cgttttgata
     3601 tcgtcgacca cccgcccacg atcgctggct gccgcctgaa aagagtaaaa gttcaccgac
     3661 cagccagacg cgggcgttcc tttactggtt aacatcgccg agaattttac cggtttatct
     3721 ttcgtccagg tctgttcgag cagttcgtaa tctgccaact gaccgaccca ggtatagaga
     3781 tcgtcactgg aagagacatc gccttccgct gaccaggtgg cctgattacc cttagcatca
     3841 agatttttta aggtgatagc atcaagcttg agaatgccgt gatactgctt tttgaattga
     3901 tcttttaaga tattttcagt tggcggttcg ccatctgccc agacattacc agtcatcaac
     3961 ataagcagcg ccgtgccaac aatccattgc tttttcaccc gaaaaccctc gcgcggaata
     4021 atttattcca gtgattatca tcagggtaat gaaaattggc aatggacgta agtctgaagt
     4081 gaaaaagccc cggcacgata ccggggcgag gtgattagta ctgattgaag atctgctgga
     4141 tctgctgcgg atctttggtt tgcgtcagag ccagctgcag caggacgcgc gctttttgcg
     4201 ggttcagcgt gccagaggcg acgaagccgt atttcgcatc atccacttca gcatcctgag
     4261 tggtagcgcc cgtcggtaca cgggaagaac gcactactgc agtgccgttt ttcgcggcgg
     4321 ttgcaagggt gtcaaatacg gttttataca ggttgccgtt acccacacca gcgctgacga
     4381 tgccatcata gcccgcatct accagtgctt tagccggaag atcggatgcg ttagcgtagt
     4441 tataaacgat gccgactttc ggcagctcat tcagcttaga gacatcgaat ggcgtatcgc
     4501 tggtgtgctt acgtgccggg gtacgttggt agtcaatctt accgttgtga atgtaaccca
     4561 gaggaccgta gttaacagac ttgaaggtcg ctacgtcggt ggtgttggtt ttggtgacat
     4621 cgcgaccgtc cagtacggtg tcgttcatca ccaccagtac gccacgatta gcggaggctt
     4681 tatcagctgc ggtcactacc gcgttataca ggttgaatgg accgtctgcg ctcatagacg
     4741 tggacgggcg cattgcaccg accatcacca ccggtttgtc gcatttcacc gtcaggtcga
     4801 ggaagtaagc ggtttcttcc atcgtgtcgg taccgtgagt aatgacgaag ccgtcggttt
     4861 tatcgcagtc ggtgttaatt tttttcgcca gtgtcagcca gacattatcg ttcatgtcct
     4921 gggagccgat attcactacc tgctcgcctt taacgttcgc aatgtccttc agttgcggca
     4981 ccgcattaac cagattttct acgccaactt tacccgctgt gtagttagat ttggttgcgg
     5041 agtcaccacc accggcaatg gtcccgccgg ttgctaaaat ggtgatattg ggtaatgcca
     5101 atgctgcacc actaaaaccc ataaccagtg cggcaagtgc cgtctttttg aaaaactcca
     5161 tttcattcct ccagttacgt gaacgctacg cattatccct tagctttgta tgggaaattt
     5221 gacgttaaac aatttacaac gtgaatatat tttggagatc tacaaagtta gaggcaggta
     5281 acaaaacgaa gaattaaacg gcataaaaaa gtattatgcc gtcttaaaat agaggattat
     5341 tttaaattcc cgaccagggc tttgcgacta tcttccagag tcacaacgcg gctacaaaca
     5401 tctttgccaa actgctggaa atctttttcc tgctttttcc attcggtttg gattgaggat
     5461 tgcagaccgc ccaggcttcc cagcacattc tgtaatgggt taccaccgct tttcagcacc
     5521 gctttcgcgc ccatttcatt aatgctgtcc tgtaaaattc cgcccattgc ctgattcact
     5581 aattgctggc cttcggcgcg aacctgatca atggctttat agtgaaacgt caggccatcg
     5641 ctgcgcgttt cgataatgcg gttcatctgc tctttcagct gcgcatcaag tttggtcaga
     5701 cggctgcgca ttttgctgct ttcgcccatc tcctgaacga taattttatc cagcgcaata
     5761 cgggctttct cgacgcggct tttcgcgcct tcatcaatcc acggcagggt gctacgcagt
     5821 tcagcctgat aatccttcgc ctgctcgcgc tgggcggcgt tcagggaata ttgcttaccg
     5881 ttatacatca cgttgccgtc tggcgtgatc accagattgc cgttttcgcc cttcacctgc
     5941 acggtttgcg ggctgacaat cacatcgtca cgcggcgtga cgctgcactg gtagtcggcg
     6001 tgagcggtca ttgccgtcac tgaaagtgct gccgccagca gcattttgcg catcatagtc
     6061 ttccctcaag aacaaatcag gccagcattt gctggcccca gattgataac aaagtacatg
     6121 tcatccatgc cggatgcggc gtaaacgcct tatccggcct actcgattgt gcatctttcc
     6181 aggcctgata agcgtagcgc atcagacagt tttgcatttg tcatcgcccg tatgttttct
     6241 tagtcccacc aaacgtcgaa aagttcgctg gtgcgtacct cgcccagttt gcgctcttcc
     6301 agccacttac gcacaatcgc ctgatgttct tcggtgcatt tgccgatttc ctgcatgcag
     6361 atcagacctt cccaggccag ataaccgctg ccgtcaaagg ccagtttgtt cggttcgata
     6421 acctcgttaa taaagtcatc aacggtttta tcaatctgtt cttccgatgt accttccggg
     6481 aatcgccatg ccaccgaaaa tcctaattcc tggaattcgt cgatgtgcat ttttttacgc
     6541 agacgacggc tacggttctt tgccattatt tcaccctctc gaacattaag tcccatactc
     6601 cgtgaccaag acgatgacca cgttgttcaa atttcgtcac cggacgtgat gccggacgcg
     6661 gtacgtaatc attgctctct gacaggtttt tataaccgtc aatagaagac atcacttcaa
     6721 gcatatgttc cgcataaggt tcccagtcgg tcgccatatg gaatacgccc cccagctgca
     6781 gtttgctttt taccagttcg gcaaacggca cctgaacgat acggcgttta ttatggcgcg
     6841 ctttgtgcca cgggtcaggg aaaaagagct gcaccatgcg caatgaattg tcaggaatca
     6901 ttttatgcag cacttcaacc gcatcgtgac acatcacgcg caggttgctt aagccctctt
     6961 catgcgcaga agccaggcac gcaccaacgc ccggtgaatg cacttcaatg ccgaggaagt
     7021 cctgctcggg gcgatcttta gccattgcca ccagcgacgc ccccatgccg aaaccaatct
     7081 caagcgtcac cggcgcttca cggccaaaaa gcgcagggaa atccagcata tcttcgctga
     7141 actcaacgcc catcaccggc cagtagtttt ccagcgcatg ttcctggcct ttggtcagtc
     7201 gcccctggcg gcgcacaaaa ctacggatac ggcgcagtgg gcggccgttt tcatcaaatt
     7261 ccggtgagat gacgtcgttt ttcataaaga tttagtcgct tgtgaaagtg ttctgaaaac
     7321 gggcattatc caaagttagt tgccggatgc aagcatgata aggccgtggc tgcggaaagt
     7381 tccggtttac accctgccgt cgctgtgctg caatcttgcc cccaacaaca gtgaattcgg
     7441 tgaccatgca agcgtcgcaa ttttcagccc aggttctgga ctggtacgat aaatacgggc
     7501 ggaaaacgct gccctggcaa attgacaaga cgccctacaa agtatggctc tcagaagtga
     7561 tgttgcaaca aactcaggtt gcgaccgtta tcccctattt tgaacgcttt atggcgcgct
     7621 tcccaacggt gaccgatctc gccaatgcgc cgctcgacga agttctccac ttgtggaccg
     7681 ggcttggcta ttacgcccgc gcgcgcaatc tgcataaagc ggcacagcaa gtggcgacct
     7741 tacacggcgg taaattcccg gaaacctttg aagaagtcgc ggcgttaccg ggcgtcgggc
     7801 gttccaccgc aggcgcgatt ctctcgcttt ctctgggtaa gcactttccg attctcgacg
     7861 gtaacgtcaa acgggtgctg gcgcgctgct atgctgtaag cggctggccc gggaaaaaag
     7921 aggtcgagaa taaattatgg agtttgagcg agcaggtgac gcccgctgtc ggcgtggaac
     7981 ggtttaatca ggcgatgatg gatttaggcg cgatgatttg tacgcgctcg aagccgaaat
     8041 gttcgctctg tccgctacaa aacggatgta ttgccaccgc caacaatagc tggtcgcttt
     8101 atccgggcaa aaaaccgaaa cagacgctgc cggagcgcac cggctacttt ctgctgttac
     8161 agcacgaaga tgaagtattg ctggcgcagc gcccgccgag tggattgtgg ggcggtttat
     8221 actgtttccc acagtttgcc gacgaagaaa gtttgcggca gtggctggcg caacggcaga
     8281 ttgctgccga taacctgacg caactgaccg cgtttcggca taccttcagc catttccact
     8341 tagatattgt gcctatgtgg cttcccgtgt cgtcattcac cggctgcatg gatgaagaca
     8401 atgcgctctg gtataactta gcgcaaccgc cgtcagttgg cctggcggct cccgtggagc
     8461 gtttgttaca gcagttacgc actggcgcgc cggtttagcg cgtgagtcga taaagaggat
     8521 gatttatgag cagaacgatt ttttgtactt tcctgcaacg tgaagcagaa ggtcaggatt
     8581 ttcagctgta ccccggcgag ctgggaaaac gcatctataa cgagatctcc aaagaagcct
     8641 gggcgcagtg gcagcacaaa caaaccatgc tgattaatga aaagaaactc aacatgatga
     8701 atgccgagca ccgcaagctg ctggagcagg agatggtcaa cttcctgttc gagggtaaag
     8761 aggtgcatat cgagggctat acgccggaag ataaaaaata aaaacagtgc cggagcacgc
     8821 ctccggcaac ttgcataaaa acaaacacaa cacgcacccg gaatgatgaa aaaatatctc
     8881 gcgctggctt tgattgcgcc gttgctcatc tcctgttcga cgaccaaaaa aggcgatacc
     8941 tataacgaag cctgggtcaa agataccaac ggttttgata ttctgatggg gcaatttgcc
     9001 cacaatattg agaacatctg gggcttcaaa gaggtggtga tcgccggtcc taaggactac
     9061 gtgaaataca ccgatcaata tcagacccgc agccacatca acttcgatga cggtacgatt
     9121 actatcgaaa ccatcgccgg gacagaacct gccgcgcatt tgcgccgggc aataatcaaa
     9181 acgttgctga tgggggacga tccgagttcg gtcgatctct attccgacgt tgatgatatt
     9241 acgatttcga aagaaccttt cctttacggt caggtggtgg acaacaccgg gcagccgatt
     9301 cgctgggaag gtcgcgcgag caacttcgcg gattatctgc tgaaaaaccg tctgaagagc
     9361 cgcagcaacg ggctgcgtat catctacagc gtcaccatta acatggtgcc gaaccacctt
     9421 gataaacgtg cgcacaaata tctcggtatg gtccgccagg cgtcacgcaa atatggcgtt
     9481 gatgagtcgc tgattctggc aattatgcag actgaatctt cctttaaccc gtatgcggtc
     9541 agccgttccg atgcgctggg attaatgcag gtggtacaac ataccgccgg gaaagatgtg
     9601 ttccgctcgc aggggaaatc cggcacgccg agccgcagtt tcttgtttga tcctgccagc
     9661 aatattgata ccggcaccgc gtatctggcg atgctgaaca acgtttatct cggcggaatt
     9721 gataacccaa catcgcggcg ttatgccgtc atcaccgcct ataacggcgg cgcaggcagc
     9781 gtgctacgag tcttttcgaa tgataagatt caggctgcca atattattaa caccatgacg
     9841 ccgggcgatg tttatcagac gctgacgacc cgccatccct ctgcggaatc tcgccgttat
     9901 ctttataaag tgaataccgc gcaaaaatct taccgccgcc gataattcca ctaaccgccc
     9961 ctgacgatgc tcaggggtaa aaatgttatc cacatcacaa tttcgttttg caaattggga
    10021 atgtttgcaa ttatttgcca caggtaacaa aaaaccagtc cgcgaagttg atagaatcgc
    10081 atcatctcgc acggtcaaat gtgctttttc aaacactcat ccgcatcacg atgtgaggaa
    10141 attaacatga atcttaagct gcagctgaaa atcctctctt ttctgcagtt ctgtctgtgg
    10201 ggaagttggc tgacgaccct cggctcctat atgtttgtta ccctgaagtt tgacggtgct
    10261 tctatcggcg cagtttatag ctcactgggt atcgccgcgg tctttatgcc tgcgctgctg
    10321 gggattgtgg ccgacaaatg gttaagtgcg aaatgggtct atgccatttg ccacaccatt
    10381 ggcgctatca cgctgttcat ggcggcagag gtcactacgc cggaggcgat gttccttgtg
    10441 atattgatta actcgtttgc ttatatgcca acgcttgggt taatcaacac catctcttac
    10501 tatcgcctgc aaaatgccgg gatggatatc gttactgact tcccgccaat ccgtatctgg
    10561 ggcaccatcg gctttatcat ggcaatgtgg gtggtgagcc tgtccggctt cgaattaagc
    10621 cacatgcagc tgtatattgg cgcagctctt tccgccgttc tggttctgtt taccctgact
    10681 ctgccgcata ttccggttgc taaacagcaa gcgaatcaga gctggacaac cctgctgggc
    10741 ctcgatgcat tcgcgctgtt taaaaacaag cgtatggcaa tcttcttcat cttctcaatg
    10801 ctgctgggcg cggaactgca gattaccaac atgttcggta ataccttcct gcacagcttc
    10861 gacaaagatc cgatgtttgc cagcagcttc atcgtgcagc atgcgtcaat catcatgtcg
    10921 atttcgcaga tctctgaaac cctgttcatt ctgaccatcc cgttcttctt aagccgctac
    10981 ggtattaaga acgtaatgat gatcagtatt gtagcgtgga tcctgcgttt tgctctgttt
    11041 gcttacggcg acccgactcc gttcggtact gtactgctgg tactgtcgat gatcgtttac
    11101 ggttgcgcat tcgacttctt caacatctct ggttcggtgt ttgtcgaaaa agaagttagt
    11161 ccggcaattc gcgccagcgc gcaggggatg ttcctgatga tgaccaacgg cttcggctgt
    11221 atcctcggcg gcatcgtgag cggtaaagtg gttgagatgt acacccaaaa cggcattacc
    11281 gactggcaga ccgtatggct gattttcgca ggttactccg tggttctggc cttcgcgttc
    11341 atggcgatgt tcaaatataa acacgttcgt gtcccgacag gtacacagac ggttagccac
    11401 taattacgca aagaaaaacg ggtcgccaga aggtgacccg tttttttatt cttacttcaa
    11461 cacataaccg tacaaccgtt tcatgccatt cgcatcggtt tcgctataga caccttgcaa
    11521 ctccggcgaa aatcccggca acaaattcac gccttcttcc agcgcaagga aataacgttg
    11581 aaccgcccca ccccagactt ccccgggtac cacgcaaagc acgccaggtg gataaggcaa
    11641 cgccccttct gccgcaattc gcccttcggc atcacgaatc cgcaccaact ccacttcacc
    11701 gcgaatataa gcgctatgcg catcctgggg attcatcacc actgacggga aactctgctg
    11761 gcggaacatc gctttttgta ggtctttgac gtcgaaactg acatacagat cgtgcatctc
    11821 ctgacacaac tgacgcaggg tgtagtcgcg atagcgcacc ggatatttgt tataaacgct
    11881 cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc tgttcaaact gcgccagcat
    11941 cgccaccagt tgtgccagct tctcgtggct ttccgccgga gttaataaga acagaatgga
    12001 gttgagatcg cacttctccg gcacaatgcc gttctcacgc aggtagtgcg ccagaatcgt
    12061 cgctggaacg caaaaatcgc tatattcgcc ggtttcggca tcgatacccg gcgtggtgag
    12121 taacagcttg cacggatcaa caaaatactg atccgcggca tatccttcaa agccgtgcca
    12181 cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg gctaacactg acgtcggata
    12241 atcctgccac aatttgccat caacaacggg cgggataaac gggcggaaca gcttacagcg
    12301 cgcaagaatc gccttccggg cttcaatccc caacgcaacg cactccgccc acagccgacg
    12361 cccactctcc ccttcatgaa ttttggcgtt aacgtccagc gcggcgaaca gcggatagaa
    12421 cgggctggta gaagcatgga gcataaaggc attattcaac cgcttatgcg ggcaaaaacg
    12481 cgcctgtcca cggatatggt tatctttttt atggatctgc gacgtctgtg agaatcccgc
    12541 ctgctgtttg tgcaccgact gggtcacaaa gatccccgga tcgttttcgt taagttctaa
    12601 cagcagcggc gagctatccg ccatcatcgg gataaactgt tcgtaaccga cccacgcgga
    12661 atcaaacaga atgtaatcac acagatgccc aacggtatcg atcacctgac gggcgttata
    12721 gacagtgccg tcataggttc ccagctgaat aatcgccagg cgaaacgggc gcggcaggtc
    12781 ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga tactcttcat taaaacagtg
    12841 cgcatcaata ccgccaatga aaccaaacgg gttacgtgaa gcttccagat agaccggcgt
    12901 cgcccccgcc tgaatcaacg cgccgtgatg attcgactta tggttgttac ggtcgaagag
    12961 caccaaatcg ccacgcgtta acagcgcatt cgtcaccact ttattcgctg ccgatgtgcc
    13021 gttcagcaca aaataggttt tatcggcatg aaagactttg gctgcgaatt tctgcgcatc
    13081 tttcgccgat ccttcatgaa tcagcagatc gcccaatttt acgtcagcgt tacacatatc
    13141 ggcgcgaaag atgttctcgc caaagaaatc gtaaaaatgg cgtccggcag gatgcttttt
    13201 aaagaacgca ccatgttgat gtccagggca agcaaaggtg ctattaccca tctcaacgta
    13261 ctgcgtcagc gtgtcataaa acggtggcag caaattctct tcatactgac aggctgcgga
    13321 ttccagctcc agccactgct gctcgttgcc gttgattacc gccgtaacgc ccgcaggtaa
    13381 ttcaacaaca tgttcggaat acaaaaacac cggtagatga aaaccggtgc gcttaagcaa
    13441 cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg actgccgcga cgtccgtaaa
    13501 atcagtatct gccaacgcca ccacgcggcg atgagtagaa agtcgggata ccagttcact
    13561 actggcggca atattcattg atttcataag cgcaaaaccc gtttcgggga agtaagaata
    13621 ccggacaagg tgaaaaaccc tgccccacga gatatgggtc aaactggtca ccagctccga
    13681 ccgccagaca tcagtaaaag cagaaacgct ctgattttac tgttgtcctg cagtgagcgt
    13741 gcgttaactt caccgcatga gcagaaaaag ggaggagttt cgcgcgaaac ggcgataagc
    13801 gagagaatgt aaggaggtgg cgtgcatcgg gcaaactccg tacagagagg agaaaattcg
    13861 cgcaatcatg gcacctttcg ctaaagcgtg caagccagga ctttgcgacc agacaagcca
    13921 tcaacaaggc aaaaagttca taataagaaa atcaaacaat acagttaaca ggaactcttg
    13981 tggtcatcgg cccttttatc aacgctagtg cagtcttact gggtggcgtt ctcggcgcac
    14041 tgctcagcca acgcttaccg gaacgtatcc gcgtctccat gacatcaatt tttggtctgg
    14101 catcgctggg gattggtatt ttactggtgg tgaaatgcgc caaccttccg gcgatggttt
    14161 tagcaacctt actcggtgca ttaatcggcg aaatttgtct gctggaaaaa ggcgtcaata
    14221 ccgcggtcac caaagcacaa aatctgtttc gccactcacg taagaagcca gcgcatgaat
    14281 cttttattca gaattatgtc gcgattattg tcctgttttg cgccagcggc accgggatct
    14341 tcggggcgat gaacgaaggg atgaccggcg atccgagtat tttaatcgcc aagtcatttc
    14401 ttgatttctt tacggcgatg atcttcgcct gctcgctggg tattgcggta tcggtgatta
    14461 gtatcccatt actgatcatc cagttaacgc tggcgtgggc tgccgcgctg atattaccgc
    14521 tgaccacacc gtcgatgatg gcagacttca gcgccgtagg cggtttattg ctgctggcaa
    14581 ccggattacg catctgtggc attaaaatgt tcccggtggt caacatgctt ccagcactcc
    14641 ttctggcaat gcccctttcc gccgcctgga ccgcctggtt tgcctgacaa tgcgtgcatt
    14701 agcggcaaag tgatgataga ttgtgcagtc tgcagtaaat tgaagaaatt tgattgacga
    14761 gacgaggcga atcaggttta atgcgccccg ttgcccggat agctcagtcg gtagagcagg
    14821 ggattgaaaa tccccgtgtc cttggttcga ttccgagtcc gggcaccaag ttcatatcaa
    14881 cggacctcca cggaggtccg tttttcgttt cagaacgcca caatttaagc gttctgcctc
    14941 caaatcaatt ctaccgaact caaccagatt ccccccacat caaccccatt gtgtgggtat
    15001 aattgcgggt ataccccagt tcgacagaat ttgtaccctc tttaacgcat tcagaaggct
    15061 gaactatggc actgactgac gcaaaaatcc gggctgcaaa gcccactgac aaggcttata
    15121 aactcactga cggggctggc atgttcctgc tggtacatcc taatggttcc cgttactggc
    15181 gtctccgtta tcgtattctg ggtaaggaga aaaccctggc acttggtgtg tatccggaag
    15241 tttctctctc cgaagctcgt acaaaacggg atgaggcccg aaaactgatt tcggaggggg
    15301 ttgacccttg cgaacagaaa agagctaaaa aagtagtccc tgatttacag ctctcttttg
    15361 aacatattgc acgacgctgg catgccagta ataaacaatg ggcacaatca cacagcgata
    15421 aagtactcaa aagcctcgag actcacgttt tcccctttat cggcaaccgg gatatcacaa
    15481 cactcagtac cccggatctg cttatccctg ttcgtgctgc agaagttaaa caaatttatg
    15541 aaatcgccag tcgtctgcag caaagaatat ctgctgtaat gcgttatgcc gtacagtctg
    15601 gcatcatcag atataacccg gctctggata tggctggtgc attgaccacg gtaaaacgcc
    15661 agcatcgccc tgctctggat ctttcacgcc tgcctgaact tctgtcgcgt attgacggtt
    15721 ataaaggcca gcctgtcacc cgactggcag ttatgctgaa tttactggtt tttattcgtt
    15781 ccagtgaact cagatatgcc cgctggtctg aaattgatat tgacaatgcc atgtggacta
    15841 ttccagccga acgcgaacct ctgccaggcg taaaattttc ataccggggc tccaagatgc
    15901 gaacaccaca tcttgtgcca ctcagccaac aggctgtagc catactggca gaacttcaga
    15961 catgggcagg tgaaaatggt ctgatcttta cgggagcaca cgacccacgt aaaccaatca
    16021 gtgaaaatac cgtaaataag gccctcagag tgatggggta tgacacaacc aaggaagtct
    16081 gtggccatgg attccgggcg atggcgtgca gtgcattgat tgaatccggc ttatggtcaa
    16141 gagatgcggt tgaacgtcag atgagtcatc aggagcgcaa cggagtacgt gccgcgtata
    16201 ttcataaagc agaacatctg gaagaacgtc ggcttatgct gcaatggtgg gcagattttc
    16261 tggatgcaaa cagggaggag tgtatcagtc cgtttgaata tgcaaaagtt aacaatccac
    16321 tgaaaaggtg actgaacgcc atttacacac atgctcttcc tcttaagggg aagagcaccc
    16381 gcctcaatca atttcttttt tatgccggcg gaaccatcag ccaggctgag gttctcattc
    16441 aataactcaa cgtcttacca cagaacagaa ttcactgtat tatcatgggc aacgttaatc
    16501 ccggacaata aaaggacacc gatgccaggc atcacaattt ttatagcctg caggttatgg
    16561 tccatccttt tttacaggtt cagaatatga ctggcaaatt acgctttgaa gtgaacgata
    16621 accagggctg tttcatattt ccggaaacct ggtttggctc gttgctggat gagtttgaag
    16681 aactgattga tgcatacgat gcggatgaaa tcagcgaaac aagttacatc aacaaactca
    16741 ggcgccttgc ccgacaggag aatgacttta ttgatgtaca cgctcacttg gcatacgtat
    16801 ttctggagca gaatgccccg cgtaaggcgc tgaatgctgc ccttaaaggg ctggcaattg
    16861 gcaatcgtct tatccctgaa ggtttcagtg gcagaatcat ctggatacat cctgacaacc
    16921 gaccttttct tcgtgccctc tatgcggcaa tcctggcaaa tgcacattta cagcgtcatc
    16981 aggatgccat catgctcatt gaaaaaatac tggattacaa tcccgaagat aatcatggtg
    17041 cacgctggct actgggaccg gaactgttac gcactggcgc tcatgaacag gcacgccata
    17101 tactgcagga acatgcagat gaattttcac cttactggta tgaactcggc cttctgcatt
    17161 ttcttaatgg ggaactggta aaagctgcaa ctgcatttcg tcgcggcttt gcggcaaata
    17221 cttacatcgc agaaatactg tgcggaaatc tgcatccatt tccacttgct gtatggcata
    17281 acttttcagg cggtccggat actgccgaag attattatgc cacataccac ccgttatggg
    17341 ggcaatatcc ggaagcattg ctgtttgtaa actggctcta taatcattcc tctgtgctgc
    17401 atgaacgggc agaaatcatt aaatgcgccg aaatgctgat gcaggaagat gatttcgaaa
    17461 tatgcgaaag tattttaaga cagcaggaga agttgcgtga aagaattgat gagacgcttt
    17521 ctgagaaaat tgtacagaaa tgcagaaata tgaatggtga atatatctgg ccctggatat
    17581 tgccgttttc agcggcaggc atgaaacata ctggcataca gtatcagtag atattgcatt
    17641 agtgtatcct gcacacaagt aataatttat tcaccaataa taacactgtt aatgccccct
    17701 tcccctggtt gtcagccagg ggttatcttc tgaatatttc ttttgaaaag gataacacag
    17761 taaattattt ttatgaatta tcccatggac tcattaacac cctttcatga tgctttattg
    17821 tcaaacacgt tatggctgac atcaaaaaaa accggatttc ctctgccagc gggtaatcac
    17881 cttcccggtg ttttcggttg gtctggttac tcctgtctgg ttattagcaa gataattgct
    17941 ataaacagtg gaaaactcat cgtacataat ctggtgatga acattacgct tattttccct
    18001 tgaccggaag aatcagaggc tgtggtttca gactgtctgc cagtacattc ctctctccgt
    18061 taaaaaccat aacgggttca ttatcttcgt ctgtcagca
//
LOCUS       DACADF010000092        18029 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.093, whole genome shotgun sequence.
ACCESSION   DACADF010000092 DACADF010000000
VERSION     DACADF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.093"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            38..1453
                     /gene="tnaA"
                     /locus_tag="ISB30_003067"
     CDS             38..1453
                     /gene="tnaA"
                     /locus_tag="ISB30_003067"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAP1990674.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDNSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1545..2792
                     /gene="tnaB"
                     /locus_tag="ISB30_003068"
     CDS             1545..2792
                     /gene="tnaB"
                     /locus_tag="ISB30_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAP1990675.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2924..4099
                     /gene="mdtL"
                     /locus_tag="ISB30_003069"
     CDS             2924..4099
                     /gene="mdtL"
                     /locus_tag="ISB30_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAP1990676.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHNA"
     gene            4074..5033
                     /gene="yidZ"
                     /locus_tag="ISB30_003070"
     CDS             4074..5033
                     /gene="yidZ"
                     /locus_tag="ISB30_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAP1990677.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSSVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNQLLDKPLHETPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLSIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            5190..5939
                     /locus_tag="ISB30_003071"
     CDS             5190..5939
                     /locus_tag="ISB30_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990678.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            5961..6527
                     /gene="yieF"
                     /locus_tag="ISB30_003072"
     CDS             5961..6527
                     /gene="yieF"
                     /locus_tag="ISB30_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAP1990679.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6580..7917)
                     /locus_tag="ISB30_003073"
     CDS             complement(6580..7917)
                     /locus_tag="ISB30_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP1990680.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8084..8749
                     /gene="yieH"
                     /locus_tag="ISB30_003074"
     CDS             8084..8749
                     /gene="yieH"
                     /locus_tag="ISB30_003074"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAP1990681.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIINIISKEQGVTMIQADAEHVYRAEVARLFDSELEVIAGANELLASVKV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8816..9283
                     /locus_tag="ISB30_003075"
     CDS             8816..9283
                     /locus_tag="ISB30_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990682.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLWVIIH
                     PIFLLLSITTGAIPALLTGVMVACLPEKIGSQKRYRCLAGGIGGVVITEIYCAVIIHI
                     KVMASSVLFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9332..9919
                     /locus_tag="ISB30_003076"
     CDS             9332..9919
                     /locus_tag="ISB30_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990683.1"
                     /translation="MTQNIRPLPLFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIANGSAAEKFAGSFQDDASIEGVEFEYDEEDDFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(9980..10702)
                     /locus_tag="ISB30_003077"
     CDS             complement(9980..10702)
                     /locus_tag="ISB30_003077"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP1990684.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKMRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGVDGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10717..11886)
                     /locus_tag="ISB30_003078"
     CDS             complement(10717..11886)
                     /locus_tag="ISB30_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP1990685.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGINHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11913..13529)
                     /locus_tag="ISB30_003079"
     CDS             complement(11913..13529)
                     /locus_tag="ISB30_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAP1990686.1"
                     /translation="MFRRNIITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSANDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13615..15009)
                     /locus_tag="ISB30_003080"
     CDS             complement(13615..15009)
                     /locus_tag="ISB30_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP1990687.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15028..16902)
                     /gene="bglF"
                     /locus_tag="ISB30_003081"
     CDS             complement(15028..16902)
                     /gene="bglF"
                     /locus_tag="ISB30_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAP1990688.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFSGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQVAQQEKTPEIITPEQGGICSPMTGDIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTVQINAGEPLLSIIR
                     "
     gene            complement(17035..17871)
                     /gene="bglG"
                     /locus_tag="ISB30_003082"
                     /pseudo
     CDS             complement(17035..17871)
                     /gene="bglG"
                     /locus_tag="ISB30_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
BASE COUNT         4571 a         4493 c         4243 g         4722 t
ORIGIN      
        1 gggatcactg taattaaaat aaatgaagga ttatgtaatg gaaaacttta aacatctccc
       61 tgaaccgttc cgcattcgtg ttattgagcc agtaaaacgt accactcgcg cttatcgtga
      121 agaggcaatt attaaatccg gtatgaaccc gttcctgctg gatagcgaag atgtgtttat
      181 cgatttactg accgacagcg gcaccggggc ggtgacgcag agtatgcagg ccgcgatgat
      241 gcgtggcgac gaagcctaca gcggcagccg cagctactac gcgttagccg agtcagtgaa
      301 aaatatcttt ggttatcaat acactattcc gactcaccag ggccgtggtg cagagcaaat
      361 ctatattccg gtactgatta aaaaacgcga gcaggaaaaa ggcctggatc gcagcaaaat
      421 ggtggcgttc tctaactatt tctttgatac cacgcagggc catagccaga ttaacggctg
      481 taccgtgcgt aacgtctata tcaaagaagc cttcgatact ggcgtgcgtt acgactttaa
      541 aggcaacttt gaccttgagg gattagaacg cggtattgaa gaagttggcc cgaataacgt
      601 gccgtatatt gttgcaacca tcaccagtaa ctccgcaggt ggtcagccgg tttcactggc
      661 aaacttaaaa gcgatgtaca gcatcgcgaa gaaatacgat attccggtgg tcatggactc
      721 cgcacgcttt gccgaaaacg cctatttcat caagcagcgt gaagcagaat acaaagactg
      781 gaccatcgag cagatcaccc gcgaaaccta caaatatgcc gatatgctgg cgatgtccgc
      841 caagaaagat gcgatggtgc cgatgggcgg cctgctgtgc atgaaagaca acagcttctt
      901 tgatgtgtat accgaatgca gaaccctttg cgtggtgcag gaaggcttcc cgacatatgg
      961 cggcctggaa ggcggcgcta tggagcgtct ggcggtaggt ctgtatgacg gcatgaatct
     1021 ggactggctg gcttatcgta tcgcgcaggt gcagtatctg gtcgatggtc tggaagagat
     1081 tggcgttgtc tgtcagcagg cgggcggtca cgcggcattc gttgatgcag gtaaactgtt
     1141 gccgcatatc ccggcagatc agttcccggc acaggcgctg gcctgcgagc tgtataaagt
     1201 cgccggtatc cgtgcggtag aaattggctc tttcctgtta ggccgcgatc cgaaaaccgg
     1261 taaacaactg ccatgcccgg ctgaactgct gcgtttaacc attccgcgcg caacatatac
     1321 tcaaacacat atggacttca ttattgaagc ctttaaacat gtgaaagaga acgcggcgaa
     1381 tattaaaggg ttaaccttta cctacgaacc gaaagtattg cgtcacttca ccgcaaaact
     1441 gaaagaagtt taattaaaac actcagagtg gctataagga tgttagccac tctattaccc
     1501 tacatcctca ataacaaaaa tagccttcct ctaaaggtgg catcatgact gatcaatcag
     1561 aaaaaaaaca ctctgcattt tggggtgtta tggttatagc cggtacagta attggcggtg
     1621 ggatgtttgc tttacctgtt gaccttgccg gagcctggtt tttttggggc gcttttattc
     1681 ttattatcgc ctggttttca atgttacatt cagggctatt attattagaa gcaaacttaa
     1741 attatcccgt cggttccagt tttaacacca tcaccaaaga tttaatcggt aacacctgga
     1801 acataatcag cggtattacc gttgccttcg ttctctatat cctcacttat gcctatatct
     1861 ctgctaatgg tgcgatcatt agtgaaacga tatcaatgaa tttgggctat cacgctaatc
     1921 cacgtattgt cgggatctgc actgccattt tcgttgccag cgtattgtgg ataagttcat
     1981 tagccgccag tcgtattacc tcattgttcc ttgggctgaa gattatctcc tttgtaattg
     2041 tttttggttc tttcttcttc caggtcgatt actccattct gcgcgattcc accagcacca
     2101 ctgcggggac gtcttacttc ccgtatatct ttatggcttt gccggtgtgt ctggcgtcat
     2161 ttggtttcca cggcaatatt cccagcctga ttatttgcta tggcaaacgc aaagataagc
     2221 tcatcaaaag cgtggtcttc ggttcgctgc tggcgctggt gatttatctc ttctggctct
     2281 attgcacgat ggggaatatc ccgcgagaaa gctttaaggc gataatctcc tcaggcggca
     2341 acgttgattc gctggtgaaa tcgttcctcg gcaccaaaca gcacggcatt atcgaatttt
     2401 gcctgctggt gttctctaac ttagctgttg ccagctcgtt ttttggtgtc acgctgggat
     2461 tgttcgatta tctggcggat ctgtttaaga ttgataactc ccacgccggg cgtttcaaaa
     2521 ccgtgctgtt aaccttcctg ccacccgcgt tgttgtatct gatcttcccg aacggcttta
     2581 tttacgggat cggcggtgcc ggactgtgcg ccaccatctg ggcggtaata attcctgctg
     2641 tactggcaat caaagcccgc aagaagtttc ccaatcagat gttcacggtc tggggcggca
     2701 atcttattcc ggcgattgtc attctctttg gcataaccgt aattttatgc tggttcggta
     2761 acgtctttaa cgtgttacct aaatttggct aaatctttca ggaagccagc cattcgctgg
     2821 cttcttgcct gtcaggaaat ctcttttgtc caaatggcaa ctcgcctgat tatccttcac
     2881 cacgtatgct ttgcgtcacc ttactatcag gacgctttag cccatgtccc gctttttgat
     2941 ttgtagtttt gccctggttt tactttatcc cgcagggatt gatatgtacc tcgttggttt
     3001 accgcgcatc gccgccgatc tcaatgccag cgaagcgcag ttgcatattg cgttctccgt
     3061 ttatctggcg gggatggcgg ctgcgatgtt atttgccggt aaagtggccg atcgttcagg
     3121 gagaaagcct gtcgccatac ccggcgcggc gctatttatt atcgcctcgg tgttctgttc
     3181 actggctgaa accagcgcgt tatttcttgc agggcgattt ctacagggac tgggcgcggg
     3241 ctgttgttac gtagtggcgt tcgccatttt gcgcgacacg ctggatgatc gacgtcgggc
     3301 taaagtgctg tcgttactca acggcattac ctgcatcatt ccggtgttag cgccggtgct
     3361 cggacacctg attatgctta aattcccttg gcagagtctg ttctggacga tggcaacgat
     3421 gggcatcgcg gtactgatgt tgtctttgtt tattttaaaa gaaacgcgcc ctgcggcccc
     3481 cgcagcttcg gacaaaccac gagaaaatag cgagtcactg cttaaccggt ttttcctcag
     3541 ccgtgttgtt atcaccaccc tcagcgtttc ggtgatcctc accttcgtca acacgtcgcc
     3601 agtattgctg atggaaatca tgggttttga gcgcggagaa tacgccacca ttatggcgtt
     3661 gaccgctggc gtcagcatga ccgtttcatt ctccacgccg tttgcgctgg gaatttttaa
     3721 gccacgtacg ttgatgatca cctcacaggt gttattcctg gcggcgggga tcacccttgc
     3781 cgtttcacct tcccatgcgg tttcgctgtt tggtatcacg ctgatttgcg ccggtttctc
     3841 ggtaggtttt ggcgtagcga tgagtcaggc gttggggccg ttttcattac gcgcgggcgt
     3901 agccagctcg accttaggta ttgcgcaagt ttgcggttcg tcactgtgga tttggctggc
     3961 ggcggtggtt ggtatcggtg catggaatat gctgatcggg attctgattg cctgtagcat
     4021 agtgagcctg ttgctgatta tgttcgtcgc gcctggacgc ccggttgccg ctcatgaaga
     4081 aatccatcac aacgcttgat ctcaatctgc tgctctgtct gcaactgctg atgcaggagc
     4141 gcagcgtgac caaagcggcg aagcggatga acgtgacccc ttcgtcggtg agtaagtcgc
     4201 tggcaaagtt aagagcgtgg tttgacgacc cgctctttgt gaactcaccg ctggggctgt
     4261 cgcccacacc gctaatggtc agcatggagc aaaatctggc ggagtggatg caaatgagca
     4321 atcagttgct ggacaaacca ctccacgaaa cgccgcgcgg tcttaagttc gaactggcgg
     4381 cggaatcgcc gctgatgatg atcatgctta atgcgctgtc gaaacggatc taccaacgat
     4441 acccgcaggc gaccatcaaa ttacgtaact gggattacga ttccttagat gccattactc
     4501 gtggtgaagt ggatattggt ttttccggtc gcgaaagtca tccccgttcg cgggagctgt
     4561 taagctcgct accgttatcc attgattatg aagtgctgtt tagtgatgtg ccctgcgtct
     4621 ggttacgcaa agatcatccg gcactgcatg aagcatggaa tctggacacc tttttacgtt
     4681 atccgcatat cagcatttgc tgggaacaga gcgatacctg ggcgctggac aatgtgttac
     4741 aggagctggg gcgcgaacgc acgattgcta tgagcctgcc ggaattcgag cagtcactgt
     4801 ttatggcagc acagcccgac aatctgctac tggcgaccgc gccgcgctac tgccagtact
     4861 acaatcaact ccaccaactg ccgttggttg ctcttcctct cccgtttgat gaaagccagc
     4921 aaaaaaagct ggaagtccct tttacccttc tgtggcataa acggaacagc cgtaatccga
     4981 agatcgtctg gttacgggaa accattaaaa atctttacgc gtcgatggca taaccgaatc
     5041 gtatgaaacg cgacccaatt tcacaatgaa atgtaaaaaa gttgtaataa atttatctga
     5101 atcgaacttt tagccgcttt agtctgtcca tcattccagt aaatgattac tcttgtattc
     5161 ataatggacc attaagcatg gagcgaaaaa tggcgactca ctttgcccga gggattttaa
     5221 cggaaggaca tctgatttct gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca
     5281 ttccccctca tcgtcaaagc cgttttctgg cgtccagagg tttactcgca gaactgatgt
     5341 tcatgctgta tggcattggc gaattgccgg aaatcgtcac cctgccgaaa ggaaaaccgg
     5401 ttttcagcga taaaaatttg ccttcgtttt ctatttccta tgccgggaat atggtcggcg
     5461 tggcgttaac aaccgaaggt gaatgtggcc tcgatatgga actacagcgt gcgacacgcg
     5521 ggtttcatag cccacacgcg cccgataacc acaccttttc cagcaatgaa tcgctatgga
     5581 tcagtaaaca aaacgatcct aacgaagcgc gggcgcagct catcacgctg cgccggagcg
     5641 tgctaaaact aaccggtgat gttttgaatg acgatccgcg cgatctgcaa ctactgccca
     5701 ttgccggacg cctgaaatgt gctcatgtaa atcatgtaga agcgttatgc gacgcggagg
     5761 acgtgctggt gtggtccgtg gcggtcacgc ccacgattga aaagctcagt gtctgggagt
     5821 tagatggcaa acacggctgg aaaagcctgc ccgatattca cagccgcgcc aacaatccta
     5881 ccagccggat gatgcgtttt gcccagctct ctaccgtgaa ggctttttcg ccaaattgat
     5941 agacaaccac aggagtcatc atgtctgaaa aattgcaggt ggttacgtta ctggggagcc
     6001 tgcgcaaagg ctcatttaat ggcatggttg cacgtaccct gccgaaaatt gctccggcga
     6061 gtatggaagt caatgcgttg ccttccattg ccgacattcc cctgtatgac gctgacgtac
     6121 agcaggaaga cggttttcca gcaacggttg aagctctggc ggaacagatc cgtcaggctg
     6181 acggtgtggt gatcgtcacg ccggaatata actactcggt accgggtggg ctgaaaaatg
     6241 ccatcgactg gctttcccgc ctgccggatc aaccgctggc tggtaaaccg gtattgattc
     6301 agaccagctc aatgggcgtg attggcggcg cgcgctgtca gtatcacctg cgccagattc
     6361 tggttttcct cgatgcaatg gtgatgaaca agccagaatt tatgggcggc gtgattcaga
     6421 acaaagttga tccgcaaacc ggggaagtga ttgatcaggg tacgctggac cacctgaccg
     6481 ggcaattgac cgcatttggt gagtttattc agcgagttaa gatctaaata aaaacccgcc
     6541 agcaatcatg catggcgggt ttttaacgcg ctatcgattt tagtgagcgt cgataaagac
     6601 aatcttcagg ataaacagca gcgcaacgat gattacgcac gggctaaggt cacgcagacg
     6661 tccggtacca atcttcatca cgcagtagga gataaagccc agcgcgatac cttcagtaat
     6721 cgagaagctg aacggcatca tcacagcggt aataaacgcc ggaacagatt cagtaagatc
     6781 ctgccagttc acgcgagcaa gacttgaggt catcaacacg ccaacgtaaa tcagcgcacc
     6841 agctgcagcg tagcctggca ccatccccgc cagcggcgac aggaagataa ccagcaggaa
     6901 cagcagacca acaaccactg ccgtcagacc ggtacgaccg ccaacggaaa cgccggaaga
     6961 agattcaata tacgccgtaa cagaagaagt accgataaac gaaccggtca ccgaagagat
     7021 actgtcgaca tacagcgcct gcttcatgcg cgggaattta cctttctcat cagccagacc
     7081 tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca aacaagttga ccagcatgaa
     7141 agagaaaatc acccctgcca gcccaaggtt aaacgacccg gctaaatcta catgacccac
     7201 gacactcatt acgctcggcg gcgcagaaac gatgccatta tagtggacat cacccagcat
     7261 ccagcccagc agcgttgtca ccacgatgga aaccagcacc gccgcgtgaa tattgcgcga
     7321 ggccagaatg gcgatgatga agaagccgag gatccccagc agcacgctgt gagaagtcag
     7381 attaccgatg ctcaccagcg tttccgggtt agcgacaatc acacctgcgt ttttcagccc
     7441 catcatgcca ataaacagac cgataccact ggtgatgccc acacgtagac ttaccgggat
     7501 gttggctatc atccagtagc gaacgcggaa aatcgtcagt aacagcagac caatcgcgcc
     7561 ccagaagatt gcgcccatcc cgacttgcca cggcaagccc atcgcctgta caacgacaaa
     7621 agcgaagaac gcattcaggc ccattgccgg tgccagtgca actggcaggt tagcaaacag
     7681 ccccatcata atactgccga atgcagcaat cagacaggtc gtgacgaaga cggcgctggt
     7741 atccatgcca gcaacgccaa gaatttgcgg gttaacaaaa acgatgtaaa ccatcgtcag
     7801 gaaggtggta aaaccggcga tcacttcggt ccgtgccgtc gtgccatgtt cgcgcagttt
     7861 aaacacgcgt tccagcatcc cctggccaga agcctgggtt gtgtgttgtt gactcattat
     7921 ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatga gacaatatgg
     7981 acggctgaag ataacttttt ttcattgtat ttgcttatac ggcaacgatt gcgtcatgcg
     8041 aattggcatg acgaaaacgt taaactaacg ataaaggaaa gacatgtccc agatagaagc
     8101 ggtatttttc gactgcgacg gtacgctggt cgatagcgag gtcatttgct ctcgcgccta
     8161 tgtcacgatg ttcagggaat ttggcattca cgttgacctg gaagagattt ttacccgttt
     8221 taaaggcgta aaactctacg aaatcatcaa catcatcagt aaagaacagg gcgtgacgat
     8281 gatccaggcc gatgccgagc acgtttaccg tgcggaagtg gcgcgcctgt ttgatagcga
     8341 gcttgaggtg attgcaggag cgaacgaatt gctggctagt gtgaaagtgc cgatgtgcgt
     8401 cgtctctaac ggcccggtca gcaagatgca gcattcgctt ggtaagctca acatgctgca
     8461 ctacttcccg gaaaaactgt tcagcggcta tgatatccag cgttggaagc ccgatccggc
     8521 attaatgttc catgcggcaa aagcgatgaa tgtgaacgtt gaaaactgca ttctggtcga
     8581 tgactccagt gccggtgcgc aatcagggat agatgccgga atggaagtgt tttacttctg
     8641 cgctgacccg cacaataaac cgatcgttca cccgaaagtc accaccttta cccatctttc
     8701 gcaattaccg gaactgtgga aggcgcgtgg ttgggatatt acggcatagt tattcacact
     8761 cccttcactt actccactta aattggcacg caaaggtaag taaagggagt ttgatatgtc
     8821 tgtttcacgt cgggtaattc atcacggact gtattttgcg gttttagggc cgttaattgg
     8881 tgttctgttt cttgtcctct acatattctt cgcaaaagaa ccgctggttc tttgggtgat
     8941 aatacatcca atttttctct tattgtcgat aactacggga gctattcctg cgttgttaac
     9001 cggtgtaatg gttgcctgtc tgccggaaaa gatcgggtca cagaaacgtt atcgttgtct
     9061 ggctggtggc ataggtggag ttgttatcac cgagatctat tgcgctgtta tcatacatat
     9121 taaggtcatg gcttcctcag tgttgtttga aaacattctt tctggtgaca gtctcgttgt
     9181 ccgcatcatt cctgcattgc tggcaggtgt ggtgatgagc agaatcatta cacgtctacc
     9241 cggactggat atttcatgtc ctgaaacaga ctctttaagt taagcgggat gttttatctt
     9301 ttggctactc aaaagcagac aggatgtttc tatgactcaa aatatcaggc cgttacccct
     9361 attcaaatat catcccaagc cactggaaac aggcgcattt gaacaggata aaaccgtaga
     9421 gtgcgattgc tgtgaacaac agacgtcagt ttattactcg ggtccctttt attgcgttga
     9481 tgaagttgaa catctctgtc cgtggtgtat tgcgaacggt tctgctgctg aaaagtttgc
     9541 aggtagtttt caggatgatg ccagcatcga aggtgttgaa tttgagtatg atgaagagga
     9601 cgattttgcc ggtattaaga acacatatcc tgatgaaatg ctgaaagagt tggttgaacg
     9661 cacgccaggt tatcatggat ggcaacagga attctggctc gcgcattgtg gcgatttctg
     9721 tgcttttatc ggctatgtgg gctggaatga tataaaagat cgcctcgatg aatttgccaa
     9781 ccttgaagaa gattgtgaga atttcggtat tagaaattct gatctagcta aatgcctgca
     9841 aaagggtggt gattgtcagg gttatctctt ccgctgtctc cactgcggca agctgagact
     9901 gtggggcgat ttttcatagt tatttatatg atgagaacag gccggcgcgt aattcacaca
     9961 tccggcctta tttttaagct taaccgagcg ctaattctgc ggcagcggct ttatcagcga
    10021 ttaccatcag cgaaggatgc agctgcaaca cggaagcagg aacatcttcg gtaaccggac
    10081 cttgcagaac atttttcagt gcctgcgctt tacccgcgcc actgacaatt atcagcaagt
    10141 ttttcgccgc catgatgctt ttcggcccca tcgtcacgta gctgtcaggc actaatgaaa
    10201 agtcgccacc caactcgcca tgcgccacaa tatcaaccat ctctccctgg atcgggaact
    10261 ccacggtttg ctcatggaaa tgggtcgtat ttggcaaatt gccacaaaaa tgaccatcta
    10321 cacctaatcc caacaccacc aaatccaggc caccttcacg cgccagtttc tgatcatgct
    10381 cgcggtagtt atcaatagtg agcttctgga tattctcttc tttgatccct gcaggggtga
    10441 aaaacagatt acgcagattg gtaatcgtta cgccttcccc ttctttgcca cgaaatggaa
    10501 tttcatcgaa attgtagaaa tagcagttat cgtaccaggg cttaccttta accagagtga
    10561 tgagatattc gtacatgcct ttgggcgtgc taccggcggt aattgccagg ttaacgcgac
    10621 gcatctttga catataacca agcagatgat gtgcggcgac acggctcatt tcctggtaat
    10681 cttcggtaat gatcaatttc atcttaatgc cttattttat ttaaataatt tctgtacaaa
    10741 ggctgcatag gctggtcgcc agacatccat ttcatggttc agaccgggat attcctggta
    10801 atcaaacttt atttttttct gctcaagctc agttttcagc ccggcgatat ccttgccggt
    10861 tacgacatct ttatcgccca cgaccacagt aaaattacgt agttgctggt taatagctgc
    10921 cggatcgttc agccgggcag cgaccccttc atctggtacg gtcgtcgtgg taacaccact
    10981 gaatgtggcc agccagccaa agctttccag atgattaatt cccgatacca gtgcctgata
    11041 cccaccttgt gaaagccctg ccagtgcgcg accatcggca tctttacgga cattaaaacg
    11101 cttgctaatc agcgggataa tatcgttcat cagttcgcga tctgccgctt tagcatttaa
    11161 cggataaaag actttacgtc tctcctgagg aacgaaatct tcgggaataa tgcccttcgc
    11221 atcggtttcg gtatccggga tcaccaccag catcggttta attttccctt cagccagcag
    11281 gttatccatg atttgcggga tacgcccctg atcaatagcg gaacgtccgg tatcaccaaa
    11341 gccgtgatag aaatagagca ctggcaaagg ctcgcctatg ccggtgtatc ctggcggggt
    11401 ccagacatac atctgacgtt cggattgcaa tgcgttggag tggtaagtta tggcgatcaa
    11461 atcaccatgc acaacagaac gtgtatccag ataactgccc ggtaccagaa tcatactgga
    11521 gttaacctga cgctgaggct tagtcattgc cgtgcctgta tcaatgctgc gtacaccatc
    11581 aacattgaaa aaatactcgt acagattgcc tttcaggaca ggcgtgcgcc acgaccagac
    11641 tcccgcttca tctttggtca tcgggtgaat attgtccgga accggaacac ccaccactac
    11701 ggagacattt tttgccccag gggcaaagta gcgaaaggtc acgctgttat cggtattgac
    11761 ctgagtcaca tactgtttca ccggaatagt gggtgccggg ctggctggca tatccgctgc
    11821 tatggcccat tgagcagaaa taccgctggc aatagccagc gttaaagcag caatttttat
    11881 attcatcgta atattccttt tattaagttc cattaccacc agatttcagc ctgggcaccg
    11941 acagcaaact ggtcgtcttt attgtcttca aaggtgaagc catccagttc gttttccaga
    12001 gctttgatat atgtagcgta gaaacgaatt tccgggcgcg aggtcaacat actggtattg
    12061 actttaaagg tatggaagag cgtggtttta taaccagact cattatattt attgctattc
    12121 gcatctttgt tttgctgggt gaaatagccc agttcaacac ctgtctggtt atattggtcc
    12181 caaatatagg ccgggcgaac aaccgcacga atagattcga aatcagaatg tgcgcctgtt
    12241 tcgtagctat aaatatcgtt accgaaggag taaacaatcg cgttagccac aatgaaatga
    12301 tcgccgatat aagcttcgcc ctgcgaagtc agacgaactg cagttccgcc tgtgtgatca
    12361 ccataataac gaccattaaa ggtagtaaat ggacttgcac cagcataacg accaaagtta
    12421 ctggcgatag agttattcgc aaccaggaag gagaattcgt tgaagccacc cttatcaaat
    12481 ttctgcgtta aagatgtacc aaacatccag gtatctttcc agtcataata cccgttatta
    12541 tcctgattat ctttttccga tgcgctttca tttgctgaga cataacgacc gctcaccatt
    12601 aaggtggctt tatcccataa cgggatatct ttatagcgta aatcaatggt attggtatta
    12661 atctgctgtt tgttttgcag gctgcgatcg taatcatcga catcttcacg aaccagcgcg
    12721 atatcaattt tacccggacc gactttccag ttttccagac ctacacctgc ggcagcatcg
    12781 gtacgctgcg ttttccagtc aagcatctgg atttcaattt tcggcgcacc gtgtttaccc
    12841 acccagaaat cagcctctgg cgcaaagggc aggaaacctt tggtggtaac gtacatatca
    12901 gagaactgca taaagttctc gccaccggcg ttatcgccaa accagccagt agagtactgc
    12961 tgacccacgt taccatccat catcacgatg gcatcaaccc gtttgccgtt ttcgttgtag
    13021 acacgttgtt ttaactgcaa atcaaaccag ccggagtatt cgttaccaaa gcggcccaga
    13081 gaaccaattg cccatgattt aggtgaacca tgggaggctg tgccccaacc agaacggtag
    13141 tagccgttgt aactaaagcc gatttcatct ttcacaaatt tactgaaatc tttcagagtc
    13201 atggaagaat aaatcgagcc tgtctcactg gcatttttct cttcattttt aaccagtaca
    13261 gcatcaggct ttgcggcacg actggtcggg aatggattgg cggcataccc ttgatcattc
    13321 gcacttacgc tacgattcac cgtcgctggc gtatactttt tcttctcttc atctttatat
    13381 tttttcagtt cgctttgcgt ttctcttaac gccttttcta atagctcaag gcgttgttcc
    13441 acggttaatg attcagccaa tgattgtgcg gaaaaggcta acggtgccat taataaaatg
    13501 gcagaggtaa taatatttcg tctaaacata atatcccttt atggtgcaaa gaaagaatta
    13561 acgcatcgca tccagactgt tctgaatgcg acgatattta aggtgcttta ttggttattt
    13621 ttttaatgat agcccccgag ttttgatcac ttctgcatac catccgaagc ttttcttgcg
    13681 cgttcttgtg aggcttcctt caccattatc atcacgatcc acataaataa agccgtagcg
    13741 cttagacatt tgtgaatgag atgcactgac taaatcaatt ggcccccaac tggtgtaccc
    13801 cataatatcc acaccatcgg caatcgcttc atttacctgt accaggtgat catttaaata
    13861 ggcaattcga taatcgtcct gtatcgaacc atccacttca acgctgtctt ttgcgcctaa
    13921 tccgttctcg acaataaata acggtttttg ataacgatcc caaagcgtat ttaacagaat
    13981 ccgtaatcca accggatcaa tttgccaccc ccactctgaa cttttcagat gcggatttgg
    14041 gatcatattc agtatgttgc cctgcgcatt tttattaatg ctttcgtcgt gggaaacaca
    14101 accggtcatg taataactaa aagagatgaa atctacggta tgttttaaat cttctgcgtc
    14161 actttcagtc atctcaatgg tgatattgtg gtcgcggaag aaacgctgca tatagccggg
    14221 atactgacca cgcgcctgaa catcaccaaa gaacatccag cgccggttct cttccatggc
    14281 ctgcaacata tcctgtggct ggcaggtgag ggggtaaacc agcccaccga gaagcatatt
    14341 gccaattttc gcttcgggga tcaggctatg acaggcttta actgcccgcg cactggcaac
    14401 cagttgatga tggatcgcct gataaacttc cgcctcgcca ctctcttctg ccagccccac
    14461 gcccgtgaat ggcgcgtgta atgacatgtt gatttcatta aatgtcagcc ataacacaac
    14521 tttatgttgg tagcgggtaa agaccgtgcg tgcgtaatgc tcgaagtgat cgatgaccgc
    14581 ccgattagcc caaccgccgt agtttttcac cagcccatat ggcatttcgt aatgggataa
    14641 cgttaccagc ggcttgatcc ccgcctgcgc catttcatca aacagccgct cgtaaaacgc
    14701 taaccccgct tcattcggtt cggcttcgtc gccctgaggg aaaattcgcg cccaggcaat
    14761 ggaaatacgc agacaggtga agcccatctc ggcaaataac gcgatatctt ccgggtaacg
    14821 gtgataaaaa tcgatggcga catctttgat attctctttc cccaggatgc gcggttccat
    14881 ttttcccatt acgccatgag gctgtaaatc tgaggtcgtg atccctttgc catcttcctg
    14941 ccatgcgcct tccacctgat tggcagctgt tgcaccaccc caaagaaatg tttctggaaa
    15001 tgctttcata attaactcct tttatcgtta gcgaatgatg gataacagcg gctcaccagc
    15061 gtttatctgc accgtgccgt ggggtaatac gtccgtaaaa tcatcgctat tactgattaa
    15121 taccggcgtc gtcagatcaa atccggcctc gcgaatagca gggatatcaa aagaaatcag
    15181 ccgatcgcct gtattgacct tgtcacccac gttgacgtga gcggaaaaga atttgccgtc
    15241 cagttttacg gtgtcgatac cgacatgaat caggatctcc acgccatcat ctgactcaat
    15301 gccaatggcg tgtaatgtgg cgaacaacga agcaattcga cccgccaccg gagaacgcac
    15361 ttcaccaaca gagggcagaa tggcaatacc tttacccaac aggccactgg caaacgtggt
    15421 atcagcgacg tgaatgagcg gcacaatatc tcccgtcatc ggtgaacaga taccgccctg
    15481 ctcaggtgta ataatctctg gtgttttctc ttgctgggca acttgcgctg gctgacgttt
    15541 agcggtgatg aaatgaagca tcaccgtacc gacaaatgcg caaccgatgg caatgacacc
    15601 accaataacg ctggcccata cggtgaaatc aatccccgtt gacgggatgg tttgcatgaa
    15661 ggtgaaaata cctggcaaac caaaggagta aactttcgtt tgcgcgtagc caatgatggt
    15721 ggcccccaat gccccactta tgcaggcgat aacgaagggg tacttacgcg gcaggttgac
    15781 gccatatact gccggttcgg tgataccaaa cagccccgtc aacgccgccg atcccgctac
    15841 cacttttttc tgcgcatcgc gttcgcagag gaagacgccg agcgccgccc cgacttgcgc
    15901 cataatggcg ggcattaaca gcgggatcat ggtgtcgtag cccagcacgg tgaagttatt
    15961 gataattaaa ggtatcaaac cccaatgcag accaaacatc acaaagattt gccagaagcc
    16021 gcccattacc gcgcctgcaa atgcaggaac cgcctgataa agccagagat aaccggcagc
    16081 aatcagttca cttatccagg ttgatagcgg ccccaccagc agaaatgtga tgggcgtgat
    16141 gaccatcaga catagcaagg gtgtgaagaa atttttgatt gccgaaggta accacgcatt
    16201 aagtcggcgt tccagaatgc tgcacaacca ggcagaaaaa ataatgggaa taaccgatga
    16261 cgagtaattc aacaatgtga ccggaatacc caggaaatcc aggcccagcg catccacttt
    16321 ttgcccgttc tcgaaggcag tcagaattaa tggatgcact aacgctccgc caatcaccat
    16381 ggcagtaaat ggattgccgc tgaagcgttt ccctgcggtg tagcccagga ttatcgggaa
    16441 gaaccaaaac aaggcatcac tggcgctaaa taaaattaaa taagtaccac tttgttcggt
    16501 cgtccactga aaagtgagtg ccagagccaa catacctttc aggatcccgg ttgccgccat
    16561 caaaccgatc agaggcgtaa aaatacctga aataacataa acaaagcggt ttaacagatt
    16621 acctttatca tcattttccg gtgcctgttg cgctttttcg ccaaggcctg ccacgctgtt
    16681 aaccgccagg aagacatcgg ccacatggtt acctatgaca acctgaaact ggccaccgct
    16741 ttccaccacc ataataatac cgggggtctt tttcagtacc tcagcttgcg ctttgctttc
    16801 atcctttaac ttaaaacgta atcgcgttgc acaatgcatc agactcacaa tgttatctgc
    16861 gcccccgact cctgcgacta ttgttctggc taactccgtc ataacttgcc ctctaccgct
    16921 ttgcggcaaa actcaaaaaa aaacctgaaa aaaacggcct gacgtgaatc aagcaatttt
    16981 tttcaggttt tgcccgctta gtgcggtaac aatcctttat tcagtaataa tatttcagtg
    17041 ttctttgcgc acgcgctcta tatttatggc taaaaacata atctctgcag gtgagatttt
    17101 acgttgatac tgcaaaccaa taaaaatggc gatccgctcc gcacattgcc atgcttgcgg
    17161 gtaattttgc tttactgctt attgtaatga ttcatcacta tcgttaatcg atgcatgttc
    17221 aataatacgc caggataaaa atttcagatg cgtaaccagt cgctgataac tcaagctttc
    17281 ttcctggtaa ttaaggctga actgaaattt tattaattgc agcatttctc gcattaattg
    17341 cgtgacacct gcaacatcct ccatatttcc gctcatttgg gcactgacca gatgcatggc
    17401 aataaagccc acttcatctt tcggtaactg cacgcccaac cgtttatcaa taatggttaa
    17461 cgcttcttcc cctagctgga actctttcgg gtaaagtcgc tggatatccc acagcagcgg
    17521 gttgggcagt agcacgtttt gctgaaagcg tttaatcgca aactggcaat ggtcagttag
    17581 cgagatataa atactgtcct gcaactttcc cagacgctcc tgcgccagag agataatacg
    17641 atcacaggtt gccatcacct caagaggcat atgacttaag agttcgctta atcgcccgtt
    17701 cagttcatga ctgctcaagg catactcttt ttctattcca cttgagttaa ttctttcgcc
    17761 tgggcgtttt tgaaagccaa ttccgcgccc catgacgact ttttcccgct gttgatcatc
    17821 aataaccacc acaacattat tgttgagaat tttggtgatt tgcatgttca tagcaaggac
    17881 cttttttata aacaaaaaaa cccgacttca ccagtattct ctggttatgt caggttttgc
    17941 ctgcgaatgc agtaacaatc cagtcattaa ttaatggttt ttataacgaa catccaggtt
    18001 cgaaaattaa tttagttgcg tgcagaaaa
//
LOCUS       DACADF010000093        17982 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.094, whole genome shotgun sequence.
ACCESSION   DACADF010000093 DACADF010000000
VERSION     DACADF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.094"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            128..631
                     /locus_tag="ISB30_003083"
     CDS             128..631
                     /locus_tag="ISB30_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAP1990689.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(698..2155)
                     /gene="nuoN"
                     /locus_tag="ISB30_003084"
     CDS             complement(698..2155)
                     /gene="nuoN"
                     /locus_tag="ISB30_003084"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAP1990690.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2162..3691)
                     /gene="nuoM"
                     /locus_tag="ISB30_003085"
     CDS             complement(2162..3691)
                     /gene="nuoM"
                     /locus_tag="ISB30_003085"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAP1990691.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAIQALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3855..5696)
                     /gene="nuoL"
                     /locus_tag="ISB30_003086"
     CDS             complement(3855..5696)
                     /gene="nuoL"
                     /locus_tag="ISB30_003086"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAP1990692.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5693..5995)
                     /gene="nuoK"
                     /locus_tag="ISB30_003087"
     CDS             complement(5693..5995)
                     /gene="nuoK"
                     /locus_tag="ISB30_003087"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAP1990693.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5992..6546)
                     /gene="nuoJ"
                     /locus_tag="ISB30_003088"
     CDS             complement(5992..6546)
                     /gene="nuoJ"
                     /locus_tag="ISB30_003088"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAP1990694.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6558..7100)
                     /gene="nuoI"
                     /locus_tag="ISB30_003089"
     CDS             complement(6558..7100)
                     /gene="nuoI"
                     /locus_tag="ISB30_003089"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAP1990695.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7115..8092)
                     /gene="nuoH"
                     /locus_tag="ISB30_003090"
     CDS             complement(7115..8092)
                     /gene="nuoH"
                     /locus_tag="ISB30_003090"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAP1990696.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8089..10815)
                     /gene="nuoG"
                     /locus_tag="ISB30_003091"
     CDS             complement(8089..10815)
                     /gene="nuoG"
                     /locus_tag="ISB30_003091"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAP1990697.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10868..12205)
                     /gene="nuoF"
                     /locus_tag="ISB30_003092"
     CDS             complement(10868..12205)
                     /gene="nuoF"
                     /locus_tag="ISB30_003092"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAP1990698.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12202..12702)
                     /gene="nuoE"
                     /locus_tag="ISB30_003093"
     CDS             complement(12202..12702)
                     /gene="nuoE"
                     /locus_tag="ISB30_003093"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAP1990699.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12705..14507)
                     /gene="nuoC"
                     /locus_tag="ISB30_003094"
     CDS             complement(12705..14507)
                     /gene="nuoC"
                     /locus_tag="ISB30_003094"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAP1990700.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMVEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14601..15263)
                     /locus_tag="ISB30_003095"
     CDS             complement(14601..15263)
                     /locus_tag="ISB30_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAP1990701.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15279..15722)
                     /gene="nuoA"
                     /locus_tag="ISB30_003096"
     CDS             complement(15279..15722)
                     /gene="nuoA"
                     /locus_tag="ISB30_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAP1990702.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETDSIANRQR"
     gene            complement(16352..17290)
                     /gene="lrhA"
                     /locus_tag="ISB30_003097"
     CDS             complement(16352..17290)
                     /gene="lrhA"
                     /locus_tag="ISB30_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAP1990703.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQTMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4527 a         4993 c         4621 g         3841 t
ORIGIN      
        1 aaccttgtta tacaacagta aataataaaa atttctcgag gcggatccag aaaaagcaac
       61 atgcgcattt tattttcttt cgtctgtttt aagatgcgaa acatgaaatt caacggagga
      121 aaaataaatg ggaatgattg gctattttgc agaaattgac tcagaaaaga ttaaccaact
      181 gcttgagtct actaaaaaac ctttgatgga taatatacat gacacgcttt caggattacg
      241 ccgactggat attgataaac gctgggattt tttacacttt ggcctgacgg gtacatctgc
      301 atttgacccc gcgaagaatg acccgctaag tcgcgcagtg cttggtgaac acagcctaga
      361 agatggcata gatggcttcc tgggattgac ctggaatcag gagttagccg cgacaatcga
      421 ccgactggaa tcactggatc gaagtgagct gcgtaaacag ttttccatta agcgactcaa
      481 tgaaatggag atttatccgg gagtaacctt ttcagaagag ttagaggggc aactctttgc
      541 ttcgatcatg cttgatatgg aaaagctgat tagcgcgtat cgacgtatgc tgcgacaagg
      601 gaatcatgcg ctgacggtta ttgttgggtg atggtgtcag ggcacccaaa aaaaccgccg
      661 aacatgtcgg cggctttctg acttacaaac tgactgctta catcagcggc attgctaacc
      721 gcacaatgct aatcagcggt tgtggccata cacccagcac cagtaccaac agtgcagaga
      781 tcagcaccac gataccgcct gcgctgtact gccagtttga tggtgcatcg cgacccggtt
      841 gttccggagc gtgaaggtat aagctcaccg ccacacgcag gtagtagtag aggccgattg
      901 ccgaaccgac aactacggca cccaccagcc accacaagtg tgcctggaca ccgaccgcca
      961 gcacgtagaa cttaccgata aagcccagcg tcattgggat accagccagt gaaagcatca
     1021 tcaccgtcat cactgccgcg aggatcggac gatgccagaa cagaccgcgg taagagaaca
     1081 gggaatcagc atccgggcca cgatacgggc tggacatcag gctgaccacg ccgaacgcgc
     1141 cgaggctgct gaacagataa cctgccaggt aaacccctac cgcttccatc gacatctcgc
     1201 cggtttgcag cgcaatcagc gccaccagca gatagccgag gtgagagata gatgagtaac
     1261 cgagcagacg tttgatattg gtctggctca gcgccatcag gttaccgaag atgatggagg
     1321 caaaggcgat aatcgccagc actacgcgaa tcgcttcact gtcacccacc ggtgcgtaaa
     1381 ggaacaaacg catcaccacg ccgaagatag cgattttgct cgccgtcgcc aggaaagtgg
     1441 aaaccggcgc aggcgcgccc tggtatacgt ctggcgtcca caggtggaac ggcaccagag
     1501 agagtttgaa gccgaggcca acaatcatca ggccgaaacc tgccagcaac agcggctcgt
     1561 tgagcatacc gtcgccgagg tttttaccca gtgcgacaaa cgacaggtcg ccagactgcg
     1621 catacaccag cgccataccg aacagcagga aagaagacgc tgcggcagaa aggatggtgt
     1681 atttgatact ggcttccagc gaacgtttct ggcggaaagc gtaaccgacc aggccaaaca
     1741 gcggcaaaga gatcagttcg ataccgagga acagagacgc cagatggttg gcattcgcca
     1801 gcaggatccc gcccagcgcg gcaattaaca ccaacaggta gaactcatcc ttgttgtcgt
     1861 tatagccttc aagccacggg taggcgaaag tacaggtggc gaggctcgcc aacaatacca
     1921 gcccggtgta aagcatggcg aaaccatcaa cgcgcatcag tggcgtaacg tccatagcgc
     1981 ccgcctggcc gacaaaccag agcgaaacca gcgccgcgtt aagcccaata accgagagcg
     2041 tagcgttgag aaaatgattg cgtcgccacg caatggagag catcacaacc accaccgtca
     2101 agccgacgat cagcaacggt agcagtgcga tcaggttttg tggagttatt gtcatggcga
     2161 tttacggcct tgtagtagta acggaattaa caaaccactg ctggatattg ccaatcgcgg
     2221 agtgcgaggt atccagaatc ggctgcggat agaagcccag cagtaccagc agcaccacca
     2281 gcaacaggat cataaacagc tcacgcagcg acatccctgg cagttcctgg ctggcaatct
     2341 ggcttttcgc tttaccgaaa taagcgcgat gcagcatcgc cagcgaataa acagatgcaa
     2401 agaccagccc aaaggtagag ataacggtaa ttaccgggac aacctggaag ctaccgaaca
     2461 gaatcataaa ttcgccgacg aagttaccgg tgccaggcat cccaagcgtt gccaccgcaa
     2521 agaacagcga cagtgctggc agccacttca tcttgctcca cagaccgccc atcatgcgca
     2581 tgtcgcgggt atggatacgt tcatagagct gaccgcagag aataaacaga cccgccgctg
     2641 acaagccgtg tgcaatcatc tggattaccg cgccctggta ggccaactgg ctaccggtgt
     2701 agatagcaat cagcacgaag cccatgtggg aaaccgaggt gtaggcgatc agacgtttga
     2761 tatcggtctg ggcgaaggcc atccacgcac catagaagat gccgataaca cccagccaca
     2821 tagcgattgg cgcgaactct gccgacgcgt tcgggaacag cggtagggag aaacgcagca
     2881 gaccgtaagc ggcggttttc agcaagatcc ccgcgaggtc aacggaaccg gcggtcggag
     2941 cctgggagtg cgcatccggc agccagccat gcagcggaac caccggcatt ttcactgcga
     3001 aggcgatgaa gaagcccagc atcaacaggt attccacgcc gttagacatc ggcgtattca
     3061 gcagctcttc atagttgaag gtccagacgc cggtcgcatt gtagtgaacg aaaaccagcg
     3121 cctggatggc gatcaacatc accagaccac tcgcctgggt gtaaatgaag aacttggttg
     3181 ccgccgtgat acgcgtttta ccgtcagagg ctttatgccc ccacagtgcg atcaggaagt
     3241 acatcggcac cagcatcatt tcccagaaga agaagaacag gaacatgtcg atggcaagga
     3301 acacgccgat aacgccgccc aggatccaca tcaggttgag gtggaagaag ccctggtatt
     3361 tttcgatctc tttccacgaa cagagtaccg ccagcacacc gagcagaccg gtcagcacga
     3421 ccatcagcaa cgacagaccg tcaatggcga gatggataga gataccaaaa cgcgggatcc
     3481 acggcatgtc gaattcagac tgccactgcg gaattccggc ggattgcgtc agtgaataac
     3541 cgccctgcaa ccacagttgc agcgacagcg ccagcgtcaa tcccatggtt accagcgcga
     3601 tccagcgcgg caccttgacg ccaaagcgtt cggtctgcca gcacaggaag ccgccgataa
     3661 agggaattaa tattagccag ggtagtaaca tggcgatctt tattccttgt aaaagtcccg
     3721 tcaggaccgg attttcaacg aattctcacg acagaaaatt ctcttcggac tgggggtagt
     3781 gtcggatgcg ccgcgaagac ggcttatccg acctacgttc ttatatgccc aggggcaatc
     3841 ccacaatcct taactcaacg cagtaccatc aacagagcca gcaccacgac cgcaccgatg
     3901 ctcatggatg ccacatacca gcgcagataa ccgttctcgc ttaacagcag acctttacct
     3961 gcaaagcggg aaaggacagc cgggatgttc atcattgagt tcagcggatc gcgtttcagc
     4021 aaccaggcaa tacccaggaa cggcttgacg aacactttgt cgtacagcca gtcaaagccc
     4081 caggcgttgt accaccaggt acccagcaga cggcccggcg cactgttggc gatggaggtc
     4141 accagagtac gtttacccag ccacagccag gcagccagca gaatgccgac caccgcgacc
     4201 acgccagagg taatttccag ggtcaacatg ctgccgtgcg ccagctccgt cgtttgcgga
     4261 agcacgccct gcagcggcgg tacaatcagt gcaccaacga aggtggaaag gatcagcagc
     4321 acaatcagcg gcaggctgtg agttacccct ttcacggcgt gagcgtgaat ttgttctttt
     4381 ccgtggaaga cgatgaaaat catacggaag gtgtagagcg aggtcataaa cgcaccgacc
     4441 agacctgcca ccatcagatt gatatgtcca ttcgccatcg cacccgcgag gatctcatcc
     4501 ttactgaaga agcccgcagt gaccagcggc agtgccgata gtgctgcgcc gcccaccagg
     4561 aagcagagat aaaccagcgg aatagattta cgcagaccac ccatcttaaa gatgttctgt
     4621 tcgtgatggc aggccagaat gacggaaccg gatgccagga acagcagcgc tttaaagaac
     4681 gcgtgggtca tcaagtggaa aatcgccgca tcccatgcct gcacgccaag cgcgaggaac
     4741 atgtagccaa tctggctcat ggtagagtaa gcgagaacac gtttgatgtc ggtctgtacc
     4801 agcgcggcaa aaccggccag cagcagcgta accgccccga caatacccac cagatgcaga
     4861 acttccggcg tcatcaggaa caggccgtgg gtacgggcga tcaggtagac acccgcggtt
     4921 accattgttg cggcgtggat cagcgcggag acaggcgtcg ggcccgccat cgcgtcggca
     4981 agccatgtct gcaacggcaa ctgcgcagat ttaccgaccg caccgcccag cagcatcagc
     5041 gtcgcccaca tcagcatgtt attgccgtca gcaaagtgcg ctggtgccag ttccaccatt
     5101 tcgcggaagt tcagggtgcc cagttcgttg taaagaatga acagcgcaaa agcgaggaag
     5161 acgtcaccca cacgggtcac gacgaacgct ttcattgccg ctgcgccatt cttcggatcg
     5221 gtgtaataga aaccgatcag cagataggag cacaggccca cgccttccca gccgaggtac
     5281 atcaacagta ggttatcggc gagaaccaga actaccatgc tggcgatgaa caggttggtg
     5341 taagcgaaga agcgagagta gccctcttct ccgcgcatat accaggaggc atacatgtga
     5401 ataaggaaac ccacaccagt gaccactgag agcatggtca gcgacaggcc gtccagcacc
     5461 agattgaaac cgatgttaaa gtcgcctacc gacatccacg tccacagcgg ctggctatat
     5521 gcctgctcac cgttagcgaa gaagtcaacg ccgataaagg cggttaccag cgccgccagg
     5581 cccacagagc caacgccgac gatcgccgag acgttttcag accagcgccc acgggagaac
     5641 gccagcagga cgaagccaat caatggcaaa ataatggtta aggcaagcat gttcatccgc
     5701 gcatctcact tactgaatcg atgttcaggt tctggcgacg acggtgaagt tgcagcagca
     5761 gcgcaaggcc gatactcgct tccgccgccg cgaggctgat ggcgagaatg tacatcacct
     5821 gaccgtcggt ctggccccag tagcttccgg cgaccacaaa ggccagcgcg gaggcgttaa
     5881 tcatgatttc cagaccaatc agcataaaca gcagattgcg acggataacc agaccggtta
     5941 agccaagaac gaataagatt gccgcgagga tcagtccatg ttgtaagggg atcatgcgtg
     6001 ctcctccgtt tttcttttcg cgctgtcgtc tttacgattg ctcagcactt cacccgcacg
     6061 ctcttcacga ccgacgtgga aggccacaac cagacccgcg agcagcagca tagaagccag
     6121 ttccaccgcc agtacgtaag gcccgaacag cgtaataccc actgctttag cactgattgg
     6181 cgtaccgtcg ataccttgat cgttaacacc gagaatggcg taaacaatca ccaccagcat
     6241 gatggctgac aaaattgccg gaccaatcca cacctgcggt ttcaaccact ggcgttcctg
     6301 ttcgatttct gaaccgccca ggttgagcat catcaccacg aacacgaaca gcaccataat
     6361 ggcacccgca tagacgataa tttccagcgc accggcaaag taagcgccca gtgagaagaa
     6421 caccccagag atcgccagca gcgaaataat caggtacagc agcgcgtgta ccggattggt
     6481 atgggtgatc actcgcaagg tcgcaagtat ggctatcagg ccacagatat aaaaagcgaa
     6541 ctccattgcc cctctcctta cggtaacagg ctcttgacgt cgataggctt ggcttcgttc
     6601 tctgcttcgc ccttatcttt gccgtcgatt gccatacctg ccatccggta gaagttatat
     6661 tccgggtatt tgcccggacc ggagatcagc agatcctctt tctcgtaaac cagatcctgg
     6721 cgcttgtatt cccccatttc gaaatccggg gttaactgaa tcgccgtggt cggacaggct
     6781 tcttcgcaca gaccacagaa aatgcagcgt gagaagttga tgcggaaaaa ttccggatac
     6841 cagcgaccgt ctttggtttc tgctttttgc agcgagatac agccgaccgg gcaggctacc
     6901 gcgcagaggt tacaggctac gcaacgctct tcgccgtccg gatcgcgggt cagaacgata
     6961 cgaccacgat aacggggcgg cagatagacc ggctcttccg ggtacattcg cgtttcgcgt
     7021 ttggcgaacg cgtgcaggcc gatcatccag atactacgaa cctgggtgcc gaaacctact
     7081 aacaattctt ttaaggtcat ggtcttattg ccccttattg cgcctgccag agaatgacag
     7141 ccgccgttac cagcaagttg atcagcgtca gcggcaggca gattttccag ccgaaggaca
     7201 ttacctggtc ataacgcgga cgcggtaacg acgcacgaat caaaatgaac atcatcataa
     7261 agaacgcggt tttcagcgcg aaccagatga atggcggtaa caacgggcct tgccagccac
     7321 cgaagaacag cgtcaccatc agtgcagaga tggtcacaat cccgatgtat tcgcccacga
     7381 agaacagacc gaacttcata ccggaatatt caatgtggta accatccgcc agttcctgct
     7441 cggcttccgg ctggtcaaac gggtgacggt gacacaccgc cacgcccgcg atggcaaagg
     7501 taataaaacc aaagaattgc gggatgacgt tccacacatg cgcctggctg ttgacgatgt
     7561 cggtcatgtt gaatgaaccg gcctgcgcca ccacgcccat caaggaaagc ccgaggaaca
     7621 cttcgtagct cagggtctgc gcagaagcac gcatcgcacc cagcagcgag tatttgttgt
     7681 tacttgacca gcccgcaaac agcaccgcgt aaaccgccag acctgccatc atcaggaaga
     7741 acaaaatccc gatgttcagg tcggcaacca cccaacccgg actgactggc acaattgcaa
     7801 aggccagcag cagcgaggta aaggcaatca tcggtgccag ggtaaagatg acgcgatccg
     7861 agaatttcgg gatccagtct tctttaaaga acattttgat catgtccgca accagctgga
     7921 gcgaaccgcc ccagccaaca cggttaggtc cataacggtt ctggaacaga cccagcaggc
     7981 gacgttcgcc aaagctcatg aatgccccac aggtgactac caccagcagg atcaccaccg
     8041 ctttaaggac ggtcagcagg atctcaatca gttccggtga tatccaactc attgttgtgc
     8101 ctccttgaga tcctcaagat gcgcgccagc cagcaccgga gcaatgccgg acatacccat
     8161 cggtaagccc acctgccctg ccgtcagccc ttcggcgatt tcaaccggca gcgtgaccgt
     8221 gttgccatcg taactaaagg agacgcgtgt acctgcgttc acccccaact tcgcggcatc
     8281 cgctgggttg agtttgatgt acggctgcgg catacggctc tggaagaccg gagcacgctg
     8341 tgacaattca tcgctgccaa acaggtggta gtacggcgcg atacgccatt tcccgtcctg
     8401 cggctggaag cgtgccggta cgctggtgaa gtaatccaga ccgttttcgc tggtttcaaa
     8461 cagacgcacg cccggatcgc caaaacgcag tttgccgccc acttcgtcct ggaatttgtt
     8521 ccacgcctgc ggggagttcc agcctggtgc ccaggcaaac ggcacttgcg aacggtgcgc
     8581 ggtcggctgg ttgttacctt ccatcgagaa ggtgaacata gtgtcaatat cctgcggctg
     8641 acgcggctca tgaacgctga tattggcgcg catggcggta cgaccgctat agcgatgcgg
     8701 ttcacgggcc agtttctgcc cacgaatacg gaatgtcgca tccggcgcag catctttgat
     8761 acctgccagt tccgggattt tcgccacaac agcgtcaatc acgtggtcga gctgcgtcca
     8821 gtccacttca cggctcagca gggtgctatg cagcgagtgc agccagcgcc agctttccag
     8881 catgacagtt ttgctgtcgt aataagccgg atcgtaaacc tggaagaaac gttgggcgcg
     8941 gccttcgttg ttgatcaccg taccgtcgct ttcagcaaag ctggcggcgg aaagtaccag
     9001 atgggcgttt tccataatcg ctgtgcgttg atggtcaacc accatcacca gcggcgcttt
     9061 agccagcgca gcattcacgc gggtagcgga agcgtgacga tgcagatcgt tttccagcac
     9121 caccaccgcg tcggcgcgtc cggtttccag ttcggttaac gcttcttcaa gcgatccacc
     9181 gcccataatg cccagcccca tgctgttgac ggaacgggca atcatggtga taccgacgtc
     9241 agcgccgcga cctttcaggg ctttcgccac gttagccgca gcctgaatca cttcagcgct
     9301 accagcgttc gtcccggaga taatcaacgg tttcttcgca ccagccagtg cctgcacgat
     9361 gacgtcgatt ttgctttgca gctcaggctc aataccgtca accgctggtg cagagttatc
     9421 cagcgcatga gcgatggcaa aacctaaacg cgcctgatct tcaaccggtg cgcggtaagt
     9481 ccacgccgcg atatcatcca gacgggtgtc atcaacgttg gtaacaaaca gcggatgctt
     9541 cgcacgttga ccgatgttga ggattgccgc aatctgccag tcagccactt tctgtgctgc
     9601 cgccatttcg cgcgctttac ctttcaccgc ctgacgcact gccagcgcga cgcgcgcgcc
     9661 ggtctgggta acgtcttcgc ccagcaccag taccgcatcg taagattcga tttcgcgcag
     9721 agccggagta taaatgccgc cttcacgcag tactttcagc gccagttgca gacgttcctg
     9781 ctcaccgtga gcgataccgg tatagaagtt ttcttcgccc accagctcac gcagcgcaaa
     9841 gttgctttcc acgctggcac gcggagaacc aataccgatc actttcttcg actgacgcag
     9901 aatatctgct gcgccctgca ttgcctgttc ggcgttgagg gtaatgaaat catcgccacg
     9961 acgctgtact ggctgacgcg gacgatcttt caggttgacg taaccgtaac cgaaacgacc
    10021 acggtcgcag aggaagtagt ggtttaccgt accgttgtaa cggttttcga tacgacgcag
    10081 ttcgccataa cgctcgccgg ggctgatgtt acagccgatg gaacattgct ggcagatgct
    10141 cggcgcaaac tgcatatccc atttacggtt gtaacgctcg gagtgcgttt tgtcggtgaa
    10201 tacgccggtc ggacaaattt cgaccaggtt accggaaaat tcgctttcca gcgtgccgtc
    10261 ttccgggcga ccgaagtaga cgttgtcgtg cgcaccgtaa acgcccaggt ccgtaccgtc
    10321 agcgtaatct ttgtagtaac gcacgcagcg gtagcaggcg atgcagcggt tcatttcgtg
    10381 agagatgaac ggccccaaat cctgattacg gtgagtacgt ttggtgaaac ggtagcgacg
    10441 gaagctgtgt ccggtcatca cggtcatatc ctgaaggtgg cagttaccgc cctcttcaca
    10501 taccggacag tcgtgcgggt ggttggtcat caaccactcg accacgcttt cacggaactg
    10561 tttcgcttct tcgtcgtcaa tggaaataaa ggtgccatcg gaagccggtg tcatacagga
    10621 catcaccagg cgaccacgcg tgtcttccgc gttttggtat tgcttcaccg cacactggcg
    10681 gcaagcaccg acgcttccca gcgccggatg ccagcaaaag taaggaatat caaggcccag
    10741 agacagacaa gcttccagca ggttgtccgc cccgttgacc tcgtattctt tgccgtctac
    10801 atgaattgta gccattagca tgcttccagt tttctcagtc agagactgag cgttaatcga
    10861 aattcggtta ccagcgctct ttcagcaggt tcggctgaat cccattaatc aaatgggtat
    10921 tgctgaacgg ctgtttgatt cccgcctcaa attcttcgcg gaaatatttg atggcgctct
    10981 gtaacggctc cactgcacca ggtgcgtggg cacagaaagt tttacccgga cctaagaatc
    11041 gacacagttg ctcaagtgtt tcgatatcgc ccggctgacc ttcaccacgc tccagcgcac
    11101 gcagaatttt cacgctccac ggcagaccgt cgcggcatgg cgtacaccag ccgcaggact
    11161 cacgggcgaa aaactcttcc aggttacgca ccagcgacac catgttaatc tcgtggtcaa
    11221 ccgccatcgc cagcgccgta cccagacggc ttcccgcttt accgatactt tcgaattcca
    11281 tcggcaggtc aaggtgcgct tcggtcagga agtcggtgcc cgcgccgcct ggctgccagg
    11341 ctttgaattt caggccgtca cgcataccac cagcgtaatc ttcaaggatt tcacgcgcgg
    11401 tggtgccgaa cggcagttcc cacagacccg gatttttcac ccgaccggag aagcccatca
    11461 gcttggtgcc agcatcttta cttttcgaga tgttctgata ccactccacg ccgttagcga
    11521 ggatcgccgg aacgttacac agggtttcga cgttgttaac gcaggttggt ttaccccatg
    11581 caccggaggt tgccgggaag ggcggcttcg agcgtgggtt agcgcgacgc ccttccaggg
    11641 agttgattaa cgctgtttct tcaccgcaga tgtagcgccc tgccccggta tggacgaaca
    11701 gttcgaaatc gaaacctgtt cccataatgt ttttgccaag cagacccgct tcggtggctt
    11761 cggcaatggc acggcgcaga ttcactgccg cttcaatata ttcgccacgc aagaagatgt
    11821 agccacgata cgctttcagc gcaaacgcag aaatgagcat accttccacc agcaggtgcg
    11881 gtagttgctc catcagcaga cggtctttat aggtgcccgg ctccatttca tcggcattac
    11941 acagcaggta acggatgttc atggattcgt ctttcggcat caggctccac ttcaggccag
    12001 tagaaaagcc cgcgccgccg cgccctttca gaccagagtc ttttacctga ttaacgattt
    12061 cgtccggaga cagcccggtc agcgccttac gcgcgccttc gtaaccgttt ttgctgcgat
    12121 attcgtccag ccacactggc tgtttgtcat cgcgcagacg ccaggtcagc ggatgcgttt
    12181 cgggagtacg gataatgttt ttcatttata ccgctccagc agttcaggga tcgcttccgg
    12241 ggtcagatgc gcgtgagtgt cctcatcgat catcatgttt ggccctttat cacagttccc
    12301 caggcagcaa gttggcagca gcgtaaagcg accgtcaaat gtcgtttgcc ctggtttgat
    12361 gttcagtttt ttctccagcg ccgcctgaat accctggtaa ccgttgatat gacagaccac
    12421 gctgtcgcaa taacggatca catggcggcc tactggctga cggaagatct ggctataaaa
    12481 cgttgccacg ccttcgacgt cgctcgccgg aatacccagc acatcggcga tcgcgtggat
    12541 cgcgccatcc ggcacccagc cacgctgctt ctgaacgatt ttcagcgctt caatggacgc
    12601 cgcacgcggg tcttcgtagt ggtgcatctc gtgctcaatc gcttcacgct ctgccgcact
    12661 cagctcaaaa gcctcggttt gtggttgttg attctcgtgc ataattagcg gtccacatct
    12721 gacataacaa aatcgatact gcccagataa acaatcaggt cagacaccag gctgccgcgg
    12781 atcgccgccg gaatttgctg caaatgcgca taactcggcg tacggatacg ggtgcgataa
    12841 ctcatggtgc tgccgtcgct ggtcaggtag taactgttga tccccttggt cgcctcaacc
    12901 atctggaagg attcattggc aggcatcacc ggaccccacg acacttgcag gaagtgggtg
    12961 atcagggttt cgatatgttg cagcgtgcgc tctttcggcg gcggcgtggt cagcggatga
    13021 tccgccttga acgggccttc cggcatgttg ttgaggcact gctcaagaat gcgcagactc
    13081 tggcgcagct cttccacttt cagcattacg cgggtgtagc agtcagaaac accaccaccc
    13141 accgggattt caaagtcgaa gttttcatag ccagaataag gacgcgcctt acgcacgtcg
    13201 aagtcgatcc cggtagcacg caggcccgcg ccagtggtgc cccactccag cgcctctttc
    13261 gcgccatagg cggcaacgcc ctgggaacga cctttcagaa tggtgttttg cagcgccgct
    13321 ttctcgtaag acgccagacg tttaggcatc cagtcgagga actcacgcag caggcgatcc
    13381 cagccgcgcg gcaggtcgtg cgctacgccg ccaatacgga accacgccgg gtgcatacga
    13441 aaaccagtga ttgcttccac cagatcgtaa attttctgac gatcggtaaa ggcgaagaac
    13501 actggcgtca tcgcgccgac gtcctgaata aaggtggaga tatacagcag gtgactgttg
    13561 atgcggaaca gttcggagag cattacgcga atgacgttaa cgcgatccgg cacggtgatc
    13621 ccagccagtt tctctaccgc cagcacataa ggcatttcgt taacgcagcc gccgaggtat
    13681 tcgatacggt cggtatacgg aatgtagctg tgccaggact ggcgttcgcc cattttctca
    13741 gcgccacggt ggtggtaacc gatgtctggt acgcagtcga caatctcttc gccatcaagc
    13801 tgcaaaacga tacggaaagc accgtgcgca gacgggtggt tcggaccgag gttgaggaac
    13861 atgaagtcct cgttttcggt gccgcgcttc atcccccact cttccggttt gaaggtcagg
    13921 gcttccatct ccagatcctg tttggctttg gtcagctcaa acggcgagaa ttcggtagcg
    13981 cgcgccgggt aatctttacg cagcgggtga cctttccagg tttgcggcat catgatgcgg
    14041 cgcaggttcg ggtgaccgtc gaatgtaatg ccaaatagat cccaggtttc acgctcatac
    14101 cagttagcgt tcgggaatag tttggtaaag gtcggtacgt gcaggtcgtt ttctgccagc
    14161 gccaccttca gcatgatgtc gcggttacga tcgatagaaa tcagatggta gaaaacggaa
    14221 aaatccgcgg caggtaaccc ttcgcggtgt gtgcgcagac gttcgtccat gccgtgtaag
    14281 tcaaacagca tgacgtaagg tttcggcagt ttctttaaga aatcgcccac ttccagtaat
    14341 tgttcacgct tgatccacac aacgggaacc ccggtgcgag tcgcctgaac agtaaaggca
    14401 tccggcccaa aacggttgcg cagttcgcca atcaccggat catcaagatg atcgcgggtc
    14461 tgccaggcgg gttcttgcgc ggttaagtcg gtcatattgt tcaccattgc aaatggtccg
    14521 tggtgactgt cggcgcagta agcttcgcga aatagattta ggaataagcg aagaaaattc
    14581 cctttgccga caggcgcaaa ttaaatctcg tcaggtgtac gcaggttggt tacggcaatg
    14641 cgttcaccgc gcttgcgttc gcgctctgat tgcatattgg cgcgataaac gccctgatcg
    14701 ccaaccaccc aggagagcgg acgacgttct ttaccgatag attcctgcaa cagcatcagt
    14761 gcctgcatgt acgcttcagg acgcggcgga cagcccggga tatacacatc aaccgggatg
    14821 aatttatcaa cgccctgcac aacggaataa atatcgtaca taccgccgga gttggcacag
    14881 gcacccattg agataaccca ttttggttcc agcatctggt catacaaacg ctgaataacc
    14941 ggtgccattt tggtaaagca ggttcctgca accaccatca ggtcagcctg acgcggcgaa
    15001 gcacgcaata cttcagcgcc aaaacgcgcc acgtcatgca ccgcggtaaa cgaagtcacc
    15061 atctcaacgt aacagcagga aaggccgaag ttatacggcc aaattgagtt tttacgaccc
    15121 cagttaacca tgtcattgag cttgcccata aacacgtttt tgttaacttc ttgctccaga
    15181 gggtcggtta cgatctcctg cttttgcagg gggtaacggt cgttctcacc gttgggatct
    15241 atgcgggtga gcgtataatc catcttaatg cctcgcggtt agcgttgacg attagcgata
    15301 ctgtccgttt ccgggttcat acgctcgcgg cgtgaacgcg cgggcgtcca gtccagcgcg
    15361 ccaatacgca ccagataaac cagacctgcc agtaacacaa aaataaaaat tgcagcttcc
    15421 acaaagccta cccagccgct ttcgcggata gaggttgacc atgcgaacag atacagcgct
    15481 tcaacgtcga agataacgaa gaacatggcc accagataaa acttggcgga caggcgtaag
    15541 cgggcggagc cgaccgagtc gataccggat tcaaacggaa cgttttttga cctcgcgcgt
    15601 gcgcgaccgc ctaaaaacca accgcctacc agcatcaggc aacacaggcc aatggcaacg
    15661 ataagaaaga tagcgaatgc ccagtgatga gcgatgactt cagtggatgt tgacatactc
    15721 attgcttact catcaaaagt agcgccagat tcactgctct tcacggcaga tggacgccac
    15781 atcgattcat ggggaggaat aaaaaaaacc ttacaataac tgcagaaact attttataca
    15841 gctaattgat gtggtctttt actcctttct ataacctttt gtcaacttta acaaaggttt
    15901 cttcacactg atttacacaa tgacaacaac atttgcattt ggcgcccttt ctccccagat
    15961 cattgacgac tccaggaaaa ttagatgttg ttgaatcgtg tccgttgtga agcaatggaa
    16021 aaaatacggg tctattttga caggaatttg tgtcgattcc tccccccaaa agagagtatt
    16081 ttcttgatct gtgacacgct tttgtcattc tataacaaaa acgcagcaac aaattcacgt
    16141 attttttaac attattgtag caggagattt ttttcaggtg attatttgtg cgtacggggc
    16201 gtgaatctct ggtgattgaa aaatgaacag ttttatacgt tctgcacgat acgaggaagg
    16261 ctttttacta aaaaaaagcc gctggggttt aaaataaacc cagcggttcg tttttacact
    16321 attgtctcag gaattatcta tcgtccgtcg attactcgat atccctttca atcaacaatg
    16381 agtcatctcc ttccggagca gacattgggc tgtattgcca cggattatgg tagctctcca
    16441 tcgtctgata gatcacctgg gccagctcat tattactgga cgggtcatag cacagcaaat
    16501 attcggtatc aggtagcggc ggtaagccat ctactccact cagcacacgc aggtccggac
    16561 tcatcatctc gaccggcctt gccgtcacgc caagaccggc tttcactgcc gcacgaacgg
    16621 ccggaagcgt cgaggcgaca taagccagtc gccagggaat atctgcttta ttaagcgtcg
    16681 ccagcaccat atcgcgaaac ggacttggat catccagcaa cacaagaggg atcggctcac
    16741 ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt tggcgaggta cgcaggttca
    16801 atgccttaaa ggtagaagga cgatgggtgg ttaccatcaa atccacttcc tgggattcca
    16861 gcatttctgc catgtaggca ttacgtttaa cgcggacatc cagcgctagt ttcggataaa
    16921 ccgaactcac gcgatttaac aggaaaggta agatcgtatc ggcagattca tctgaagcac
    16981 cgatagttaa cacgccctga agattactga acattaatga tgagcaggcc tcatcattaa
    17041 aacgcaggat tttcctggcg taaccaagaa gttgaatgcc atgttcagtt aacagtttgt
    17101 tgcgaccgtg acgagcgaac agttctttcc caacgagttg ttccagacgc tgcatttgct
    17161 gacttacggc ggactgagta cgacacacag cggcagctgc ggcagcaaaa gtgttcagat
    17221 cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag gttaattatc ggacgatttg
    17281 cacttatcat atattatcac ttactggcgg ctcatactga gctggttaat gctgtgcaca
    17341 cacaaacaag caattccatt tgtaatgtgc ctccctggca gtttcatccg gaacccggac
    17401 gaaagtaaaa atgcatatga gttgcactaa aaaagcgact cacattgttc cgttataatg
    17461 cctgaagtag atcacagaat atatcttcag ggatcgcata tctattaagt tactcactct
    17521 tttctattta tgacatgcgc gtgtttgtat aaatgtaaat gtgagtcctt gttccactct
    17581 cgtacagcat cgctggtcat acgcgaacac gtacaaacag caatggtgag gcggcatcaa
    17641 gagcagggat ccgtcattta tgcaagcatt ttaccccaaa aaccttttat ttataagggt
    17701 cattgcgaat tatctgatgc aaagttatgt tatgttaggc aaagtaatct tctattatta
    17761 ataagcacaa caaaaccttt ttgaatatta aataataatt aattagcttc atccccattc
    17821 attaattccg cttaacaata gtttcacacc ttgcccccat tgtgacacct cggaaatcat
    17881 cacgctgtta aactctccgt aatgcggaaa tatcatcatc gcgattcagt agtgaattct
    17941 taaacgaggc attttcacac aattatctta cagataaaaa aa
//
LOCUS       DACADF010000094        17716 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.096, whole genome shotgun sequence.
ACCESSION   DACADF010000094 DACADF010000000
VERSION     DACADF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.096"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(306..896)
                     /locus_tag="ISB30_003098"
     CDS             complement(306..896)
                     /locus_tag="ISB30_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990704.1"
                     /translation="MRTPSRYIFRLPSHEINPFRATLLLILLICAVLAGVSWLILSFV
                     RTGNTFIFWLTLFIGYLIAIAKQEKIKLIEKRQIMADKRQGLSICQFARQFSPHTVDT
                     WVIRAVWNTLQGNGYIDYPLPLKASDKLDDDLDLVNDADELEELVEDIAARCGRDLRG
                     IEDNPFLPITTVGSLVSVLNAQPMTQERRSLLFTRS"
     gene            complement(946..2142)
                     /gene="hemY"
                     /locus_tag="ISB30_003099"
     CDS             complement(946..2142)
                     /gene="hemY"
                     /locus_tag="ISB30_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAP1990705.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2145..3344)
                     /gene="hemX"
                     /locus_tag="ISB30_003100"
     CDS             complement(2145..3344)
                     /gene="hemX"
                     /locus_tag="ISB30_003100"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAP1990706.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQADAP
                     AAAPQGE"
     gene            complement(3366..4106)
                     /gene="hemD"
                     /locus_tag="ISB30_003101"
     CDS             complement(3366..4106)
                     /gene="hemD"
                     /locus_tag="ISB30_003101"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAP1990707.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKCALILRGNGGRELIGDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(4103..5044)
                     /gene="hemC"
                     /locus_tag="ISB30_003102"
     CDS             complement(4103..5044)
                     /gene="hemC"
                     /locus_tag="ISB30_003102"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAP1990708.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSLIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            5431..7977
                     /gene="cyaA"
                     /locus_tag="ISB30_003103"
     CDS             5431..7977
                     /gene="cyaA"
                     /locus_tag="ISB30_003103"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAP1990709.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            complement(8017..8337)
                     /gene="cyaY"
                     /locus_tag="ISB30_003104"
     CDS             complement(8017..8337)
                     /gene="cyaY"
                     /locus_tag="ISB30_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAP1990710.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8385..8648)
                     /locus_tag="ISB30_003105"
     CDS             complement(8385..8648)
                     /locus_tag="ISB30_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990711.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDEPEHNIYQK
                     QGKGVVYLRPYEKTNLSLYRDIIIPVTDESCRNAAADGQTLKP"
     gene            8807..9010
                     /locus_tag="ISB30_003106"
     CDS             8807..9010
                     /locus_tag="ISB30_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP1990712.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9047..9871
                     /gene="dapF"
                     /locus_tag="ISB30_003107"
     CDS             9047..9871
                     /gene="dapF"
                     /locus_tag="ISB30_003107"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP1990713.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGIGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9868..10575
                     /locus_tag="ISB30_003108"
     CDS             9868..10575
                     /locus_tag="ISB30_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAP1990714.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            10572..11468
                     /gene="xerC"
                     /locus_tag="ISB30_003109"
     CDS             10572..11468
                     /gene="xerC"
                     /locus_tag="ISB30_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP1990715.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIIHFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            11468..12184
                     /gene="yigB"
                     /locus_tag="ISB30_003110"
     CDS             11468..12184
                     /gene="yigB"
                     /locus_tag="ISB30_003110"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAP1990716.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            12268..14430
                     /gene="uvrD"
                     /locus_tag="ISB30_003111"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             12268..14430
                     /gene="uvrD"
                     /locus_tag="ISB30_003111"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAP1990717.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(14485..15387)
                     /locus_tag="ISB30_003112"
     CDS             complement(14485..15387)
                     /locus_tag="ISB30_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HAP1990718.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(15470..16234)
                     /locus_tag="ISB30_003113"
     CDS             complement(15470..16234)
                     /locus_tag="ISB30_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HAP1990719.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            16604..17554
                     /gene="corA"
                     /locus_tag="ISB30_003114"
     CDS             16604..17554
                     /gene="corA"
                     /locus_tag="ISB30_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAP1990720.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
BASE COUNT         4070 a         4643 c         4714 g         4289 t
ORIGIN      
        1 tcaggaagtt attactcagg aagcaaagag gattacagaa ttatctcata acaagtgtta
       61 agggatgtta tttcccggtt ctctgtggca taataaacga gtagatgctc attccatctc
      121 ttatgttcgc cttagtgcct cataaactcc ggaatgacgc agagccgttt acggtgctta
      181 tcgtccactg acagatgtcg cttctgcctc atcagacacc atggacacaa cgttgagtga
      241 agcacccact tgttgtcata cagacctgtt ttaacgcctg ctccgtaata agagcaggcg
      301 tttttttatg accgagtaaa caacaggcta cgtcgctctt gggtcatcgg ttgtgcgttt
      361 aatacactta caagagaacc taccgtggtg atgggaagaa acggattatc ttctattccg
      421 cgtagatcgc gcccacaacg agcggcaata tcttcgacca gctcttctaa ctcgtctgca
      481 tcgtttacta aatccagatc atcgtccagt ttatcactgg ctttcagtgg taatggataa
      541 tcgatatagc cgtttccttg taaggtattc cacacagccc gaatcaccca tgtatctacg
      601 gtgtgggggg aaaattggcg tgcgaactga caaatggaaa gtccctgacg tttatcagcc
      661 ataatctggc gcttctcgat gagcttgatt ttttcctgct tcgcaatggc tatcagatac
      721 ccaataaaaa gtgttaacca gaagatgaat gtattgcctg tacgtacgaa ggacagaatc
      781 agccaactta cgccagccag aacggcacat atcaacaaaa tcagcaagag cgttgcccga
      841 aaaggattaa tttcatgtga tggtaagcgg aaaatatagc gggacggtgt gcgcatgttt
      901 gacatccttg taaaaaaata tcgcccctga acgaggcgat atagattact gcggtgggtt
      961 gttttgcaac gttagcatca aaccgtcgcg acgcatcgct gcggcttctt ccggcttgtg
     1021 cagtctgtcc agcgcgtcgg caagccatgc gtaatcgtag gcgtccggac gttgtttcag
     1081 cgctgcgcgg aaggcgagcg atgcttcctg ccattctccg tgtttcatca gcgactgacc
     1141 cagtgtgctc cacaacagcg ggcgatcgcc cacgtttttg atctgttggc gcagcacttt
     1201 ttccagctgt tccggattat tggttttcag acgcgggatc ggcagcagca ggcgatcgtc
     1261 atactggcgt ttcaggccgt cgatgataat ttgctgggca gtatcatgat cgtcacactc
     1321 aataagatgt tccgccattg ccacctgcaa ggccacctga tgacgcgttt tccggctttg
     1381 gtttttccac cagttacgca aaccttcgct accgttatcg gcacgcgcct gatccatcag
     1441 gccaatccat gcctgttgtt ccagcattgc acgatgttct tcatcaccaa catgggcttt
     1501 cgccattgat gggatgatat ccagcagcga actccatgcc cctgtgcgga tatacgcctg
     1561 ttccgccaga cgtaatactt ccggatggcg tggcgtaact tccagcagct tatcaacgcc
     1621 gtgacgtgct gcatggtttt cattacgggc cagttgcaga cgtacacggg tgatttctac
     1681 cggaatggtg tcattgccgg ccagctccgc tgcgcgttcc agatgttggt tggcgcgtgc
     1741 ttcatcacca cgttgttgcg cggcttcggc tgccagtaga tagttcacca ccggttgttc
     1801 agcgtgatcg gcatttttcg ccatcagctt ttcaacttgc tgataatcgc cttccgccag
     1861 tttcagcagc gcctgttcgg tctgcttacg tgcacggcga cgcttacgtc cgacaaacca
     1921 cccacgggtg tgcgcgcctg tgcggaagat tcgccgcagt agccactcaa tggcaaacag
     1981 cactaccatc gccagaatca aaataatcgc gaggcccgtg acgctagttt caatattgta
     2041 gttgtcggtc tggatcagca cgtaaccctg atggccggca atcatcgggc caaccacgat
     2101 ccccgcaatc agcaacacaa agagcaataa cacttttagc atggttattc tccttgcggc
     2161 gcggctgccg gagcatcagc ttgcggtgca ggtgcaggtg caggtgcagg tgcaggttta
     2221 gcttccgttg tccctgctgc cggttgtgcc agcaggttac gcacgcgggt ctgcatcaat
     2281 ttttccagca tcgcctggct ttgcagggtt tccggaagat ccatcgagat attttgctgg
     2341 cttaactggt ccacctcgtc gaggaacgct ttggtggtgg catcatcagt atcgtagtaa
     2401 gcacgtaccc aggtggagac gttctccagc gcctggcgat aagtctcttc ctggtgacgc
     2461 ggtacagctt gtgctgcgac cagcaggcga gagcggatat tttcgcgcag atagatatcc
     2521 tgatttggcg ctaacagcgg tacggcagtg tcatcacgac ggcgaatcgt aatgaagttg
     2581 tccataaaat tctgccagct tttttgcaga ttgatacgcc attcgctgat ggaactggaa
     2641 agctcttcac cgtcggaatc catcggcgaa ccatcgctgt cattatcggc cagacgcaga
     2701 ttatctacct gatttgaaag ctgattaagc ttaaggatga taccgtcata atccacctgc
     2761 gataccgcag aaaggctggc gatatcatcg gtaattgccc ggcgaacggt aatcagactc
     2821 gggtcattca tatccgccag gctggcgtct gcacttttca gcaacgctgc agcggtcgtg
     2881 acgtcctgat cgctccacag cttccgtccg gcgagtttca ccagaaaatc ggcctgggcc
     2941 agcagccagg ttttagcatc gctgccggaa atggtggcga ccttttgttg gacttcatcc
     3001 agctgttttg ccagcgtttc ttgctgacga ttcgcctgct caagctgtac agcttgttgc
     3061 ttgataatgc cttccagctc ggctttttgg ctctcctggg ctttttgcag tgccgtaagt
     3121 tggttagcca gggcatcgct ggtggcagtc tgattgacgg cctgttgttt accccagcca
     3181 tacaaaccga cgcccgccgc cagagcaata gcgatagcca ccgcgctgag aatcaatgcg
     3241 gtattgttct tactcttttt ttctgttgcg acaggttgtg acgtggtgtc cacggcctcc
     3301 ctggtctctt caaccacggc ggaggttttt tcttgttccg tcattatggc ttcctgttat
     3361 gagagttatt gtaatgcccg taaaagcgca tcgttgtcag cgttatcggc gaccttaatg
     3421 tcttgccagc ccagttcccg ggcgagtttc gccaaacgct cactgacgac caatagtcga
     3481 cagcgtagta accagtgctc acgataccat tgtgggatca gcgaccagag ttgctgcaac
     3541 atttcaccgc tggtaacaac gaccgtcgtc acctcgcggg attgccagcg catcgcttct
     3601 tctgcaccat cgtaatggat tgcgcatcgt tgataacatt cacaaaaagt gacctcagca
     3661 ccgcgcgccg tcagggaatc cccaattagc tcacgaccgc cattgccacg taatatcagc
     3721 gcacatttgc ccgcaatatt ttgtaattca ggtaattgta gcaagacttc gctgatttcc
     3781 cgatcctgtg ggtagagaat cttctgtccg cttacggtat gtagtgccag cgcggtggtg
     3841 cgtccaatgg cgaaataagt aggtagtcgg ggccatttac gatcttgctg atgcagctgt
     3901 gattgggcaa aagcaaccgc gtgttgcgag agggcaaaca acagatcgct ctctcccagc
     3961 gctgccagtt gatcagcaag ttgcggtaat tgtcgacccg gagaaaactc aatcagcgga
     4021 aaatgccagg ccacctgccc cagtgtgcgc agacggctca ctaactcttc tcctgcggga
     4081 gacgggcggg tgaccaggat actcatgccg gggcgtctcc gttatagact tcagcgagga
     4141 tctcgcgcgc gccgttattc agtagctctt ctgccagcga aatccccatt tgttcggcat
     4201 cttgcggcgc accgcggcgt tcaccgcgaa taatcagcga accgtccggc gcgccgacca
     4261 acgcacgcag ccagatttcg ccatcaataa gctcggcgta gctaccaatt ggcacctgac
     4321 atccgccttc gagacgggta ttcatggcgc gttcggcggt aacacgcagt gcagtttcgt
     4381 ggtgattcag cgcggcgagc agctcgcgag tgcgtgcatc atcaaggcgg cattcaatgc
     4441 ccaccgcacc ttgccctacc gccggaagag aaatctcggg tggcaacgcg gcgcgaatgc
     4501 gggactccag acctaaacgt tttagtccgg ctacggcaag aatgatggca tcgtattcac
     4561 cgttatccag tttgctcagg cgagtgccga cgttgccgcg cagggagcgg ataatcagat
     4621 ccgggcggcg ttcagccagt tggcactggc gacgtaaact ggacgtcccg acgatactgc
     4681 ctgccggtaa cgcatccaga ctgtcatagt tattggacac aaatgcatcg cgaggatctt
     4741 cacgctcgca aatagtgacc agccccagac cttgcgggaa ttcaaccggc acatctttca
     4801 tcgaatgtac ggcgatatcg gcgcgatttt cgaggagcgc gacttccagc tctttaacaa
     4861 ataagccttt tccgcctact ttcgccagcg gcgtatcaag aatcacatcg ccgcgcgtca
     4921 ccatcggtac cagttcaacg accaggcccg gatggctcgc catcaacttg tctttgacat
     4981 agtgtgcctg ccagagtgca agtgggcttt ggcgtgtggc aattcttaaa acattgtcta
     5041 acatgcttgt taccgtcatt atcatccgtg gtccatccta acatccttgc cagagcgatg
     5101 tcagtgttgt ggtgaaacgt agacgcctgc gcaaaccgta aaatgaggtc gggcagtgga
     5161 tcctgacagg cgctttgagc cttcgtaata aggaatttac agagaataaa cggtgctaca
     5221 cttgtatgta gcgcatcttt ctttacggtc aatcagcaag gtgttaaatt gatcacgttt
     5281 tagaccattt tttcgtcgtg aaactaaaaa aaccaggcgc gaaaagtggt aacggttacc
     5341 tttgacatac gaaatatccc gaatgccgcg tgttaccgtt gatgttggcg gaatcacagt
     5401 catgacgggt agcaaatcag gcgatacgtc ttgtacctct atattgagac tctgaaacag
     5461 agactggatg ccataaatca attgcgtgtg gatcgcgcgc ttgctgctat ggggcctgca
     5521 ttccaacagg tctacagtct actgccgaca ttgttgcact atcaccatcc gctaatgccg
     5581 ggttaccttg atggtaacgt tcccaaaggc atttgccttt acacgcctga tgaaactcaa
     5641 cgccactacc tgaacgagct tgaactgtat cgtggaatgt cagtacagga cccaccgaaa
     5701 ggtgagcttc caattactgg tgtatacacc atgggcagca cctcgtccgt agggcaaagt
     5761 tgttcctctg acctggatat ctgggtctgt catcaatcct ggctcgatag cgaagagcgc
     5821 cagttgctac aacgtaaatg tagtctgctg gaaagctggg ccgcctcgct gggtgtggaa
     5881 gtcagcttct tcctgattga tgaaaaccgc ttccgtcata atgaaagcgg cagcctgggg
     5941 ggcgaagatt gtggctccac ccagcatata ctgctgcttg acgaatttta tcgtaccgcc
     6001 gtgcgtctcg ccggtaagcg tattctgtgg aatatggtgc cgtgcgacga agaagagcat
     6061 tacgacgact atgtgatgac gctttacgcg cagggcgtgc tgacgccaaa tgaatggctg
     6121 gatcttggtg gcttaagctc gctttctgct gaagagtact ttggtgccag cctttggcag
     6181 ctctacaaga gtatcgattc cccatacaaa gcggtgctga aaacactgct gctggaagcc
     6241 tattcctggg aatacccgaa cccacgtctg ctggcgaaag atatcaaaca gcgtttgcac
     6301 gacggcgaga ttgtatcgtt tggcctcgac ccgtactgca tgatgctgga gcgtgttact
     6361 gaatacctga cggcgattga agatttcacc cgtctggatt tagtgcgtcg ctgcttctat
     6421 ttaaaagtgt gcgaaaagct cagccgtgaa cgcgcctgtg taggctggcg tcgcgcagtg
     6481 ttgagccagt tagtgagcga gtggggttgg gacgaagctc gtctggcaat gctcgataac
     6541 cgcgctaact ggaagataga tcaggtgcgt gaggcgcaca acgagctgct cgacgcaatg
     6601 atgcagagct accgtaatct gatccgcttt gcgcgccgca ataaccttag cgtctccgcc
     6661 agcccgcagg atatcggcgt gctgacacgt aaactgtacg ccgcgtttga agcattaccg
     6721 ggtaaagtga cgctggtaaa cccgcagatt tcgcccgatc tctcggaacc gaatctgacc
     6781 tttatttatg taccgccggg ccgcgccaac cgttcaggtt ggtatctgta taaccgcgcg
     6841 ccaaatattg agtcaatcat cagccatcag ccgctggaat ataatcgcta cctgaataaa
     6901 ctggtggcgt gggcatggtt taacggcctg ctgacctctc gcacccgttt gtatattaaa
     6961 ggtaacggca ttgtcgattt gcctaagttg caggagatgg tcgcagacgt gtcgcaccat
     7021 ttcccgctgc gcttacctgc accgacaccg aaggcgctct acagcccgtg tgagatccgc
     7081 catctggcga ttatcgttaa cctggaatat gacccgacgg cagccttccg caatcaggtg
     7141 gtgcattttg atttccgtaa gctggatgtc ttcagctttg gcgagaatca aaattgcctg
     7201 gtgggtagcg ttgacctgct gtatcgcaac tcgtggaacg aagtgcgtac gctgcacttc
     7261 aacggcgagc aatcgatgat cgaagccctg aaaactattc tcggcaaaat gcatcaggac
     7321 gccgcaccgc cagatagcgt ggaagtcttc tgttatagcc agcatctgcg cgggttaatc
     7381 cgtactcgcg tgcagcaact ggtttctgag tgtattgaac tgcgtctttc cagcacccgc
     7441 caggaaaccg ggcgcttcaa ggcgctgcgc gtctccggtc aaacctgggg cttgttcttc
     7501 gaacggctga atgtatcggt acagaaactg gaaaacgcca tcgagtttta tggcgctatt
     7561 tcgcataaca aactgcacgg cctgtcagtg caggttgaaa ccaatcacgt caaattaccg
     7621 gcggtggtgg acggctttgc cagcgaaggg atcatccagt tcttcttcga agaaacgcaa
     7681 gacgagaatg gctttaatat ctacattctc gacgaaagca accgggttga ggtgtatcac
     7741 cactgcgaag gcagcaaaga ggaactggtg cgtgacgtca gtcgcttcta ctcgtcttcg
     7801 cacgaccgtt tcacctacgg ctcaagcttc atcaacttca acctgccgca gttctatcag
     7861 attgtgaagg ttgatggtcg tgaacaggtg attccgtttc gcaccaaatc tatcggtaac
     7921 atgccacctg ccaatcagga gcatgacgcg ccgctgttgc agcagtattt ttcttgatgc
     7981 taatgccgga aagcgaggct tatccggcat tagctcttaa cgaaaactga cggtttctcc
     8041 cgcctgctgc gtcgccgcct gttccagcaa atcccagaag gtttcgccgc tgcgatcgca
     8101 aatccactca tcgcctttca gatcaaaatg gtagccgccc tgtttggttg ccagccatac
     8161 ctggtgcagt ggttcctggc ggttgataat gattttgctg ccgttctcaa aggtaatggt
     8221 tagtacaccg ccgttgattt cacagtcgat atcgctgtcg ccatcccagt cgtccagacg
     8281 ttcttcaatg gtcagccaca gttgatcagc caggcgatga aattcactgt cgttcattgt
     8341 tgtatcctgt ttttaagtga tggcggcagt atagcggcat ggggtcaggg cttcaaagtt
     8401 tgcccgtctg ctgctgcgtt ccggcatgat tcatccgtca ccggaataat aatatctcta
     8461 tatagcgaaa gattggtctt ttcataaggg cgcaaataca ccacgccttt tccctgtttc
     8521 tggtaaatat tatgttccgg ctcatcaagc cgacatcccc atgccgatga taagaacaac
     8581 gaacaagaca ataaacaaac cacattgcga tagtgcataa agccatcctg gcgcgaggtg
     8641 ccgatcacga aactaccagc aaaacataaa tcgccacgag taagcgttat actcgcagca
     8701 tttcctcact tttcagactt cataaagagt cgctaaacgc ttgcttttac gccttctcct
     8761 gcgatgatag aaagcagaaa gcgatgaact ttacaggcaa tccataatga aaaacgtgtt
     8821 taaggcactc actgtattac ttactctctt cagcctgacg ggctgcggtc tgaaaggtcc
     8881 gctctatttc ccgcctgcag ataaaaacgc accgccgccg accaaaccgg tagagacgca
     8941 aacgcaatcc acggtgccgg acaaaaacga ccgcgccact ggcgatggtc catcccaggt
     9001 gaattactaa aagtcagttt ctgtacccgc gtgattggag taaatgatgc agttctcgaa
     9061 aatgcatggc cttggcaacg attttatggt cgtcgacgcg gtaacgcaga atgtcttttt
     9121 ttcaccggag ctgattcgtc gcctggcgga tcggcacctg gggatagggt ttgaccagct
     9181 gctggtggtt gagccaccgt atgatccgga actggatttt cactatcgca ttttcaatgc
     9241 tgatggcagt gaagtagcgc agtgcggcaa cggtgcgcgc tgctttgccc gttttgtgcg
     9301 cctgaaaggg ctgaccaata agcgcgatat ccgcgtcagc accgccaacg gacggatggt
     9361 gttgaccgtc accgatgatg atctggtccg cgtcaatatg ggcgaaccca acttcgaacc
     9421 ttctgccgtg ccatttcgcg ctaacaaagc ggaaaagacc tatattatgc gcgccgccga
     9481 gcagacaatc ttatgcggcg tggtgtcgat gggaaatccg cactgcgtga ttcaggttga
     9541 tgatgttgat actgcggcgg tagaaacgct tggtcctgtt ctggaaagcc acgaacgttt
     9601 tccggagcgc gccaatatcg gttttatgca agtggttaag cgcgagcata ttcgtttacg
     9661 cgtttatgag cgtggggcag gagaaaccca ggcctgcggc agcggcgcgt gtgcggcggt
     9721 ggcggtaggg attcagcaag gtttgctggc cgaagaagta cgcgtggaac tccctggcgg
     9781 tcgtcttgat atcgcctgga aaggtccggg tcacccgtta tatatgactg gcccggcggt
     9841 acatgtctac gacggattta ttcatctatg aagcaaccag gggaagaact gcaggaaaca
     9901 ctcacggagc ttgatgaccg ggcggttgtc gattatctga ttaaaaatcc tgagtttttt
     9961 atccgcaatg cgcgcgcagt agaagcgata cgtgtgccgc atccggtacg cggcaccgtt
    10021 tcgttggtcg agtggcacat ggcccgcgca cgtaatcata ttcatgttct ggaagagaac
    10081 atggcgttgt tgatggaaca ggctatcgcc aacgaaggcc tgttttatcg tctactctac
    10141 ctgcagcgca gtctcaccgc cgccagcagt ctcgatgata tgctgatgcg ctttcaccgc
    10201 tgggcgcgcg atctcggcct ggcaggtgcg agtctgcgcc tgtttccaga tcgctggcgc
    10261 ttaggtgcgc cgtcgaacca cactcatctg gcattaagcc gtcagtcttt cgaaccgctg
    10321 cgtattcagc gtctggggca ggaacagcac tatctgggac cgcttaacgg accggagctg
    10381 ctggtggtgc taccggaagc gaaagcggtg ggatcggtgg cgatgtcgat gctgggaagc
    10441 gatgctgatt tgggggttgt gctgtttacc agtcgcgatg ccagtcacta tcaacaaggg
    10501 cagggaacgc agttacttca tgaaattgcg ctgatgttgc cggagcttct ggcgcgttgg
    10561 attgaacgcg tatgaccgat ttacacaccg atgtagaacg ctacctacgt tatctgagcg
    10621 tggagcgcca gcttagcccg ataaccctgc ttaactacca gcgacagctt gaggcgatca
    10681 tccattttgc cagcgaaaac ggcctgcaaa gctggcagca atgtgatgtg acgatggtgc
    10741 gtaattttgc tgtacgcagt cgccgtaaag ggctgggagc tgcaagtctg gcgttacggc
    10801 tttctgcgct acgtagcttt tttgactggc tggtcagcca gaatgaactc aaagctaacc
    10861 cggcgaaagg tgtttcggca ccgaaagcgc cgcgtcatct gccgaaaaac atcgacgtcg
    10921 atgatatgaa tcggctgctg gatattgata tcaacgatcc cctcgccgtg cgcgatcggg
    10981 caatgctgga agtgatgtac ggcgcgggtc tgcgtctttc cgagctggtg gggctcgata
    11041 tcaaacacct cgacctggag tctggcgaag tgtgggtgat gggaaaaggc agcaaagagc
    11101 gccgcctgcc gattggtcgc aacgccgtgg cgtggattga gcactggctt gatttgcgcg
    11161 acctgtttgg tagcgaagac gacgcgcttt ttctgtcgaa actgggcaaa cgcatctccg
    11221 cgcgtaatgt gcagaaacgc tttgccgaat ggggcataaa acaagggctg aataatcacg
    11281 ttcacccgca taaattacgt cactcgttcg ctacccatat gctggagtcg agcggcgatc
    11341 ttcgtggtgt gcaggagctg ctgggtcatg ccaacctctc caccacgcaa atctatactc
    11401 atcttgattt tcaacacctt gcctcggtgt acgatgcggc gcatccacgc gccaaacggg
    11461 ggaaataatg cgtttttacc ggcctttggg gcgcatctcg gcgctcacct ttgacctgga
    11521 tgataccctt tacgataacc gtccggtgat tttgcgcacc gagcgagagg cgcttacctt
    11581 tgtgcaaaat tatcatccgg cgctgcgcag cttccagaat gaagatctgc aacgcctgcg
    11641 ccaggcggta cgggaagcgg aacctgagat ttatcacgac gtgacgcgct ggcgttttcg
    11701 ttcgattgaa caagcgatgc tcgacgccgg gctgagtgcc gaagaagcca gtgcaggcgc
    11761 acatgcagca atgatcaact ttgccaaatg gcgcagccgg atcgacgtcc cgcagcaaac
    11821 ccacgacacc ttaaaacagc tggcgaagaa atggccgctg gtggcgatca ccaacggtaa
    11881 cgcccagccg gaactgtttg gtttggggga ttattttgag tttgtgctgc gcgctggccc
    11941 gcacgggcgc tcaaaaccgt tcagcgatat gtacttcctg gcggcggaaa aactcaacgt
    12001 gccgatcggc gagatcttac atgtcgggga cgatctcacc actgacgtgg gtggggcaat
    12061 tcgcagcgga atgcaggcct gttggatcag accggaaaat ggcgatctga tgcaaacctg
    12121 ggacagccgt ttactgccgc atctggaaat ttcccggttg gcatctctga cctcgctgat
    12181 ataatcagca aatctgtata tatacccagc tttttggcgg agggcgttgc gcttctccgc
    12241 ccaacctatt tttacgcggc ggtgccaatg gacgtttctt acctgctcga cagccttaat
    12301 gacaaacagc gcgaagcggt ggccgcgcca cgcagcaacc ttctggtgct ggcgggcgcg
    12361 ggcagtggta agacgcgcgt actggtgcat cgtatcgcct ggttgatgag cgtggaaaac
    12421 tgctcgccgt attcgattat ggcggtgacg tttaccaaca aagcggcggc ggagatgcgt
    12481 catcgtatcg ggcaactgat gggcactagc cagggcggta tgtgggtcgg caccttccac
    12541 gggctggcgc accgcctgct gcgtgcgcac catatggacg ccaacctgcc gcaggatttc
    12601 cagatcctcg acagtgaaga ccagctacgt ctgcttaagc gtttgatcaa ggcgatgaat
    12661 ctcgacgaga agcagtggcc gccgcgtcag gcaatgtggt acatcaacag ccagaaagat
    12721 gaggggctgc gtccgcatca tattcagagc tacggtaatc cggtggagca gacctggcag
    12781 aaggtgtatc aggcgtatca ggaagcgtgt gaccgcgcgg gcctggtgga cttcgccgag
    12841 ctgctgctac gtgctcacga gttgtggctt aacaagccgc atatccttca gcactatcgc
    12901 gaacggttta ccaatatcct ggtggacgaa ttccaggata ccaacaacat tcagtatgcg
    12961 tggatccgcc tgctggcggg cgataccggc aaagtgatga tcgttggcga tgacgatcag
    13021 tcaatctacg gctggcgcgg agcgcaggtg gagaatattc agcgtttcct caatgatttc
    13081 cccggtgctg aaactatccg tctggagcag aactaccgct ctaccagcaa tattctgagc
    13141 gccgcgaacg ccctgattga aaacaacaac gggcgtttgg gtaaaaaact gtggaccgat
    13201 ggcgcggacg gtgagccgat ttccctttat tgcgctttta acgaactcga cgaagcgcgt
    13261 tttgtggtta atcgcatcaa aacctggcag gacaacggcg gggcgctcgc tgagtgcgcc
    13321 attctctacc gcagcaacgc ccagtcgcgt gtgctggaag aggcgttatt gcaggcgagt
    13381 atgccgtatc gtatttacgg cgggatgcgc ttcttcgaac gccaggaaat caaagatgcg
    13441 ctctcgtatc tgcgcctgat tgccaaccgc aacgacgacg cggcctttga gcgtgtggtg
    13501 aatacgccaa cgcggggtat tggtgaccgg acgctggacg tggtacgtca gacatcgcga
    13561 gatcgccagt taacactctg gcaggcatgt cgtgaactgt tgcaggaaaa agccctcgcc
    13621 ggacgtgctg ccagcgcctt acagcggttt atggagctga tcgacgcctt agcgcaggaa
    13681 actgccgata tgccgctgca tgtacagact gaccgggtaa ttaaagactc cggcctgcgc
    13741 accatgtacg agcaggagaa gggcgaaaaa ggtcagacgc gtatcgaaaa cttagaggaa
    13801 ctggtgacgg caacgcgcca gttcagctac aacgaagaag acgaagattt aatgccgctg
    13861 caggcattcc tctcccatgc ggcgctggaa gcgggcgagg ggcaggcgga tacctggcag
    13921 gacgcggtgc agttgatgac gctacactcg gcgaaaggtc tggaattccc gcaggtgttt
    13981 atcgttggta tggaagaggg catgttccca agccagatgt cgctagatga aggcgggcgt
    14041 ctggaagaag aacgccgtct ggcctacgtt ggcgtaaccc gcgcgatgca gaaactgacg
    14101 ctgacctacg cggaaacccg ccgtctatat ggcaaagagg tttaccatcg cccgtcgcgc
    14161 tttatcggtg agttgccgga agagtgtgtg gaagaggtgc gcctgcgcgc cacggtaagc
    14221 cgcccggtca gccatcagcg tatggggacg ccgatggtcg agaacgacag cggctacaag
    14281 ctcggccagc gcgtacgcca cgctaagttt ggtgaaggca ccatcgtcaa tatggaaggc
    14341 agcggcgagc atagccgttt gcaggtggca ttccagggcc agggtattaa atggctggtg
    14401 gcggcttacg cccgactgga gacggtgtaa tttacgattc cgttgccgga tgcggtgcgc
    14461 tacaccgcat ccggcatcag caccttactt agcgcacttc ttcacatcaa tattccagtc
    14521 agcaccagac gcaacctgat gtttctgcgc gaaattatcc atattcagcg ccacggaaac
    14581 attcaataag ctgttcagat acaccatcgg ctggccttcc ggcacatcgc cgaagcttgc
    14641 gacatacggc attttccctg cgtatttttg ctgggaacct tcggaaatgg tcacacacaa
    14701 cgtgtcgttg agtttgattc ctgcctgatt cagcaactcg tcgctgatat tgctccagac
    14761 gttgccgtac tgaatatcga gaatcgggat attgcccttc acttcacctt ttgttgcttt
    14821 ggctttctga taagaaagtt cgacaacttt tgcaggaagc tctggcccca cctgctcgaa
    14881 cgtgatcgcc ccggaagcca gacgcgcacc ggtgtaggca tatacatcac gaccatggaa
    14941 ggtatatgac ttttcggaac ctttcagacg gttggttttt tcatcgattt gccggacgct
    15001 ctcaataccc agcgattccg ccaccaacgt gagcgtaccg ttatctggtg aaacgaagta
    15061 ctgaccgttt ttggttttca gcactaccga tttacggtct gtaccaacac ctggatcgac
    15121 cacgctaaca aacaccgaac cttgcggcca gtaactggca gtctgataca ggcgataagc
    15181 gccttcccag atgttatacg gtggaatttc gtgtgttaaa tcaaagattt taagattatg
    15241 gtcaacaccg aatgcgaccc ctttcatcgc cgagacggca ccatctttca ggctaaaatc
    15301 cgtttgtaaa atcaaagcgt tatccgcata agcggatgat gaccccagac aggttaataa
    15361 aaataatgtt gcgagcgttt ttttcatttt tgtttccctt catttatctg atgattttcc
    15421 agctgtgtag ccagccccag tttgtctggc ctcctcgagt gtacaatgtt taacctttct
    15481 gctcgacgga aatcatggtg acaaaaggat agcgttgcca cgggattgcc tcgcctttca
    15541 tatacatatg tgaaattgtg ccatcaagat aaagcagctg ctcaacgttc agtttcgctt
    15601 tggcataaca ggcaaaatca taaaaatttg tcgcctgctg gctcagcaaa aacacggcgt
    15661 tcccatgttt attaatccca acaccgttac gaattttacg tgaggcgacg ttgggatgaa
    15721 tacgcggatt aattacaccg ttttccatca acaatggccc tgactgcacc gcaaactgaa
    15781 tctctttact ggttttaaag gcatccagac gaacgatgcc gactttatct cccgcgacat
    15841 aaaacacgcc gccagggcga ataaagaaat tcccttcacc tgaagcgaga tttaacgcca
    15901 ccttctgctg accgttttcg atgtacaaac cgagcggcgc atagctttca tcatagatgc
    15961 cgccgttcat cgccatctgc acctgaccct gactattaat atccgccagc agcgcatgta
    16021 acgttcccca cgcttcgcca ttggcttttt gccagtacat tttcacccgc tctgtttgag
    16081 gattaacggt atacgcctgt acggtcagcg tcggatctga gggtgcgcaa tcatcagcgg
    16141 caacagcaaa caagggaagt aaggtgaggg tgagaaaaat ccgtttgaga ttcaaggtga
    16201 tcattccttt accaatgaat agccgatgcg ccattatagg ccctgaatgt gggatttttt
    16261 tatcctgtta gcgaccttga cgagtaccaa aaagcgcgaa gttcaactat tgttctgtgg
    16321 tgttctgttg cgtgttgacg gcaaaatttt gctggcgtaa catgcgcgca cgatcactct
    16381 aagaggacat tcgccttgga cacacccagt agatactggc tcactatcct gtcatccagg
    16441 atcaactcct aaggctatcc ctttttgctg atagccttag cggttgtcag cgacctcaat
    16501 ttttcccgtc gcgctgagtc aggctgttta atggtctgaa acccaatttg tttctgtgtg
    16561 cccaccgaac tgtccgatat tttaagcatt gggagtcccg gtcatgctga gcgcatttca
    16621 actggaaaat aaccgactga cccggctgga agtcgaagag tcacaacccc ttgtaaatgc
    16681 agtatggatt gatcttgtcg aaccggacga cgacgagcga ctgcgcgtac aatctgaact
    16741 tggccagagc ctggcaaccc gcccggaact ggaagacatc gaagcatcgg cacgtttctt
    16801 tgaagacgat gacggcctgc atattcactc cttcttcttc tttgaagatg cggaagatca
    16861 cgccggtaac tccactgtgg catttaccat ccgtgatggt cgtctgttta ctctgcgtga
    16921 gcgtgaactg cccgcttttc gtctgtatcg tatgcgtgcc cgtagccagt caatggtgga
    16981 cggtaacgcc tacgagctgc tgctggatct gttcgaaacc aaaatcgaac agttggcaga
    17041 tgaaattgaa aatatctaca gcgacctgga gcagttgagc cgggtaatta tggaagggca
    17101 tcagggcgat gagtacgacg aggcgctctc cacgctggcg gaactggaag atatcggctg
    17161 gaaagttcgc ctgtgtctga tggataccca gcgcgcgctc aacttcctgg tacgtaaagc
    17221 acgcttaccg ggtgggcaac tggagcaggc gcgtgaaatc ctgcgagata tcgaatccct
    17281 gctgccgcat aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg cggcgatggg
    17341 ctttatcaat atcgagcaga accgcatcat caaaatcttc tcggtggtat ccgtggtgtt
    17401 cctgccaccg acgctggtcg cttccagcta cgggatgaac tttgagttta tgccggaact
    17461 gaagtggagc ttcggctacc caggcgcgat tatctttatg atcctcgcgg gcctggcacc
    17521 gtatctgtac tttaagcgga agaactggct gtaaaaaacg agaacggtgg cttagcctgg
    17581 ctaagccacc gattatttaa aggctccagg tatgaatacc ttttacttct ttttcataga
    17641 accatctgtt ggtattgtat gaaacataag cggcgataat gaatccaaat acattatagc
    17701 caatcatgta accaaa
//
LOCUS       DACADF010000095        17640 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.097, whole genome shotgun sequence.
ACCESSION   DACADF010000095 DACADF010000000
VERSION     DACADF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.097"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            202..1101
                     /gene="yegS"
                     /locus_tag="ISB30_003115"
     CDS             202..1101
                     /gene="yegS"
                     /locus_tag="ISB30_003115"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAP1990721.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRITWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1183..1962)
                     /locus_tag="ISB30_003116"
     CDS             complement(1183..1962)
                     /locus_tag="ISB30_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1990722.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2062..3102)
                     /gene="gatD"
                     /locus_tag="ISB30_003117"
     CDS             complement(2062..3102)
                     /gene="gatD"
                     /locus_tag="ISB30_003117"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP1990723.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3150..4504)
                     /locus_tag="ISB30_003118"
                     /pseudo
     CDS             complement(3150..4504)
                     /locus_tag="ISB30_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            complement(4508..4792)
                     /gene="gatB"
                     /locus_tag="ISB30_003119"
     CDS             complement(4508..4792)
                     /gene="gatB"
                     /locus_tag="ISB30_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAP1990724.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVERSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4823..5275)
                     /gene="gatA"
                     /locus_tag="ISB30_003120"
     CDS             complement(4823..5275)
                     /gene="gatA"
                     /locus_tag="ISB30_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAP1990725.1"
                     /translation="MTNLFVRGGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVLFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPEPQLKEYFTKYVLDSDE"
     gene            complement(5285..6547)
                     /gene="gatZ"
                     /locus_tag="ISB30_003121"
     CDS             complement(5285..6547)
                     /gene="gatZ"
                     /locus_tag="ISB30_003121"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAP1990726.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCADDSIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLNYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQAAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENSSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6576..7430)
                     /gene="gatY"
                     /locus_tag="ISB30_003122"
     CDS             complement(6576..7430)
                     /gene="gatY"
                     /locus_tag="ISB30_003122"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAP1990727.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAP
                     VIIAGTPGTFTHAGTENLMALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7657..8709)
                     /gene="fbaB"
                     /locus_tag="ISB30_003123"
     CDS             complement(7657..8709)
                     /gene="fbaB"
                     /locus_tag="ISB30_003123"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAP1990728.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEESSAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            8966..10243
                     /locus_tag="ISB30_003124"
     CDS             8966..10243
                     /locus_tag="ISB30_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1990729.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10240..11244
                     /locus_tag="ISB30_003125"
     CDS             10240..11244
                     /locus_tag="ISB30_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP1990730.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILGWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            11241..12206
                     /locus_tag="ISB30_003126"
     CDS             11241..12206
                     /locus_tag="ISB30_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1990731.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNREWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12180..12926)
                     /locus_tag="ISB30_003127"
     CDS             complement(12180..12926)
                     /locus_tag="ISB30_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP1990732.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQSLEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEDVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(12978..13796)
                     /locus_tag="ISB30_003128"
     CDS             complement(12978..13796)
                     /locus_tag="ISB30_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAP1990733.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTEEELQGFVDGIKETP"
     gene            complement(13861..14661)
                     /gene="thiD"
                     /locus_tag="ISB30_003129"
     CDS             complement(13861..14661)
                     /gene="thiD"
                     /locus_tag="ISB30_003129"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP1990734.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14658..15446)
                     /gene="thiM"
                     /locus_tag="ISB30_003130"
     CDS             complement(14658..15446)
                     /gene="thiM"
                     /locus_tag="ISB30_003130"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAP1990735.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTRPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMASAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15481..15577)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15669..15941)
                     /gene="rcnR"
                     /locus_tag="ISB30_003131"
     CDS             complement(15669..15941)
                     /gene="rcnR"
                     /locus_tag="ISB30_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAP1990736.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16062..16886
                     /locus_tag="ISB30_003132"
     CDS             16062..16886
                     /locus_tag="ISB30_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAP1990737.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGTTLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17105..17443
                     /gene="rcnB"
                     /locus_tag="ISB30_003133"
     CDS             17105..17443
                     /gene="rcnB"
                     /locus_tag="ISB30_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAP1990738.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17525..>17640)
                     /locus_tag="ISB30_003134"
     CDS             complement(17525..>17640)
                     /locus_tag="ISB30_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAP1990739.1"
                     /translation="TDTTVSIPITASYYVYDTNKVKSGALEATALINVKYD"
BASE COUNT         4326 a         4579 c         4274 g         4461 t
ORIGIN      
        1 tattaggtat attctctttt ttaaaattta tcagtggcta ccgttaatat tcgctgtccc
       61 cattgtaagg tcctggtggt aagcactgaa tccccatacc tgaactgact ttttatcctc
      121 cgactttcat cctgttctga ctccaccttt tcttttctgc tctacactat ctacagacca
      181 atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggc
      241 aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg
      301 acgatccatg tgcggatcac ctgggagaaa ggcgatgctg cacgatttgt agaggaggcc
      361 cggaagttag gcgtcgcaac agtgattgcc ggtggtggcg atggcaccat taatgaagtt
      421 tctacggcat tgattcaatg tgagggggat gacatacccg cgctgggaat tttaccatta
      481 ggaactgcca atgattttgc cactagtgta gggattcctg aggcactgga taaggcgctg
      541 aaactggcaa ttgccggtaa cgccattgcg atagatatgg cgcaggtcaa caaacaaacc
      601 tgttttatta atatggcgac aggcgggttt gggacgcgta ttaccacaga aacgccggaa
      661 aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggac
      721 actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc
      781 ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcaacaatt gtgcccgaac
      841 gcattaatta acgatggctt gctgcaactg cgcattttta ccggcgatga aattattccg
      901 actctcgtat caaccttaaa atctgacgaa gataacccga atattatcga aggtgcttcg
      961 tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg cgaaccgttg
     1021 agtgggcaaa acttccatat tgaaatactt ccggcggcgt tgcgttgtcg attaccacca
     1081 gattgtccat tgttgcgtta atcaaactat tctctgttga accagagaga atccgtaatg
     1141 ccggatacgg tgtaaacacc ttatccggca tacagaacaa tactacaccg taattaattt
     1201 tacccgtgtc ttctgcaatg ccagtttatc cacctcagac acagcatcat cggtaataat
     1261 aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacgattaa atttcgatga
     1321 atcaagaacg gcaatcactt tattcgccgc tgtcaccatc gcaccgctaa cggaataacc
     1381 ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga caaacattaa
     1441 atcggcatta atatcctgca gggaacgttc agcaatagat ccatgcatcg agcgcgtttt
     1501 atggcggacc gtgccaccgc agacgaccag agtaatgtct ttattttcgg aaagggcaaa
     1561 cgccgccggg agactgttgg taatcaccgt gatatttttg gcggtcatta atccttcggc
     1621 gataagcatt gtggtacttc cgctgtcgag aataacggtc atcccttcgt ggatcatttt
     1681 gactgccgcc tgcgctattc tgtttttcgg cgcgctggcg agctgaaatc gctctttaaa
     1741 cccgacttcc tgggtctcgg tttcgctatt acctgacatt attttcgccg caccgccatg
     1801 aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc
     1861 cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt cattcactaa
     1921 ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat attcaattgt
     1981 ggctagtgta aacgaagggg aaccattggt gaatgatctg ttttgcacaa taccgggcca
     2041 gcatcacgct ggcccgcggt ttcagggaat gagcaacact ttgcccggca tagcattacg
     2101 agcgatgtca cgcaccgcct gggcaaagct ttcaaagctt ccacggtgag cgattaatgg
     2161 ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg
     2221 ccaagggctg gagtagttca tccaactgcc gataaccgtc agctctttac gcaatatttt
     2281 gccaaacgtt gcagatgtta aatgcagatc ctgatgcaac gtgcccacca gcgccagttg
     2341 ggcatgagga ccggcaatct ctaccgccag ttcgacggtt tgcgggacgc cagccgtctc
     2401 gaggataagc tgattaaagc gcagctcgcg taaaacgccc tgtatttgcg gcgcgctcat
     2461 ttcacggctg ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca gtttttctga
     2521 gctaatgtcg atagccgtca cactctttgc tcccagcgcg acagcgcact gaattgccag
     2581 caggccaatg gttccggcac caataataat aacgttttta ttctcacatc cttgcgctaa
     2641 atggaacgca tgcaggccaa cggtaatcgg ttcaataaaa gcgccatcct caataggcat
     2701 atccgcaggt agtgcaaaga cattgtttcg cttaacgaca atatattcgg caaatccacc
     2761 atcacgccgt gagccaataa aatcatattt tgcgcactgg gaataaaacc ctttcagaca
     2821 ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc cggggtgtaa
     2881 atcatcaaca ccggatccca ccgcatcaat atagccacta aattcatggc ctaacgttat
     2941 tggataataa tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac ataagcccga
     3001 gctggcaatt tttacccgca cctcatcctg atgtttaatt tcaggaatga cgctttctgc
     3061 tacgcgcacg ataccatcag tatcattcac cactgatttc ataaaaactc ctgattgtta
     3121 agggggataa cctccccctc aggtaaaaat tattctgcga gaacggcttt ctcttgttta
     3181 ataaaaccac gcgctctacg ccaggtcatg aaaataccgg taagataaat tgcgccgata
     3241 acaatgaaac cgggaatatt ttgcggggag aaaacctgaa tcagtaacca ggtaatggga
     3301 gaaccgccct gatccattga agccaccata cccccggctt ttaacgcgcc cgcattagcc
     3361 gccagttggg tgtgcaaacc aatagtttgc gtcgcgatcc acagggtgat gctcataatg
     3421 atgacacccg agattaaggt gcggaacaga tttccacgat gcaccgcgac cgccatcgcc
     3481 acaaagaagc cgatggtggc aaggtcgcca aacggcagca cctgattacc cggcacacaa
     3541 acagcaatta aaatggtgag tggaataaaa atcaggcttg ccgataccac cgccgtatgt
     3601 cccagcagca acgccggatc aaggccaatc aggaactcct gaccgccgaa cttcgcctgt
     3661 aaacgactac gggcctgctt agcgatgggc gttaaaccat ccatgatggg tttaatcacc
     3721 cgtggcatca gcagcataac tgccgccgtt tttaccgcca gctgcaatac acctttgaca
     3781 tcgtaacccg cgaggatgcc gataatcagc cccatcacaa aacccacggt gaccggttcg
     3841 ccaaaagggc caaatttgcg ctgaatatca tcggcgctaa atttaattcg gttaacgcct
     3901 gggatttctc gatgatagca tcgaccagca cagcaattgg ccccatatac gccgacgtac
     3961 cgtgcggaat agcaatgcct tccagctcaa agaaatcacg ggtatcgcgg gcaaaccagt
     4021 cgccgagctt ataaacaaac gccgcgtgaa ttaccacacc cgccatccct atcatccatg
     4081 aaccggttgc cagatgcagc aacgcgccgg tgaaggtcat atgccagata ttccagatat
     4141 caacatttac cacccgcgtc atacgggtca gtaacatcgc cacgttaacc agaatcgcaa
     4201 tcggaatcgc caccagcgca atttgcgacg cccaggtcat gggtgaagag cccggccagc
     4261 cgacatcgac cacatgcaga ttcaggtcga aattttccgc catcgctttc gccgccgggc
     4321 caatggaatc cagcattaag ccaatcacaa ggccaatgcc aacaaagcca atcccgatat
     4381 gcagacccgc tttaaagcaa tcgcctgcct tcatgcctaa tattttagaa aaaataatga
     4441 tgacaatcgg cagcatcacc gtagggccga ggtcgagaat ataacgcatg acttctgaaa
     4501 acataggtca cccctgtaag atagtcagaa ttttattttg taatgcttcg ataccgacac
     4561 cagaaacaaa aggcatgccg tgaactaacg gaatatcgcc aaaactgcgt tcaactttgg
     4621 cggtagtaca tatcaaatgc acgccatcca tataggtttc tatttcatta acccgacact
     4681 ggattaattc aacaggaata ttatgactct gacacaactc tttaatttct tccgccgcca
     4741 tcgtagaggt cgctaccgcg cctccgcaag cgacaataat tttgcgtttc ataaatagtc
     4801 ctttttattg ttacagaggg atttattcgt ctgaatctaa aacatacttt gtgaagtatt
     4861 cctttaactg gggttcagga agagtgatta gtgtctcgac gatatcgggc tgttgtaact
     4921 tgccaaataa acagcgtaaa agtttcaatt gctgctgcgg attttccaca atcaacgcaa
     4981 taaccaacga taccgccacg tcgttatcat catccgcttg ctgaaaaaga actttatttg
     5041 ttggccttaa cagataaatg gctgacgact tagcatgaat cgcctcacaa tgcggtattg
     5101 caatggcgtg ctgctcaagc attatcccgg tagggaattc tgcttctctg gcaattaatg
     5161 cctgtggcca ggtatcatga accacacctt tcgcgagcat ctcattaccg atatgggtta
     5221 aaacttcgct acgatcgaca aaagaaattc cgccacgaac aaacaggtta gtcatatacc
     5281 gtccttattc cgcacagccg tagcgatagg cgcgcaaaac atcataaatt ttatccataa
     5341 taagctgatg cggtattgct gataattccc cggactgaat gcgttcaaat tgtttcggaa
     5401 gatactgact aatcatgccc agtgggatgt ctacgccttc cagattcacc atcatcgttt
     5461 cgacgctatt tttaatccgg ctatgcggcc agtaataacg aatacgatcc gacaggctgt
     5521 aacgaatatc cagtaatgaa tcgttaaaac ccgtgcgata atatttcttc cagtactgcg
     5581 gttcgtcgag catcacttct tcaattaccg ccaggcaacc actgctattt tcgggggcga
     5641 taagttcctg ctcaatttgc gccagcgcaa atatcgcctc gcgtaacgca aaggttaatg
     5701 ccggaccgac tttcaaaatt gcaaaatgat cgcgaactaa ttcccgatag gccgcctgag
     5761 tctggtaatc ggtggaatgc gcttcataaa ccattttggt tttttctatc cattgcgcca
     5821 acgcctgagc ttcttgcgcc tgataatgaa taatattgct atggtcaaat tccacaccag
     5881 gctgcaccac gatggcaatc acgcgtgtta acgcctctgc cagaccgcgg gcaataaacg
     5941 ctttttgatg cgtgcgtaaa gtattggcgg catcttcaac ctgggtgatg tgtactgact
     6001 gaatggcgct ggcctcaccg cccgggaccg gaacttcagt gccaatgaca tagttcagtt
     6061 gctcacgctg gcaatcagtc gccacacttt ccgccgccag acaaagcaca gcggcacgtt
     6121 cagcaaccgt ttctggcgct aacggtatgg aatcgtcagc gcaggacatt gacgcatcaa
     6181 gatgaatttt actgaagccg gcacgaacat atgcctttac cagctcgacg gatttttcca
     6241 tcgccgcatc cgcattttct tgctgccagc agtttggtcc cagatggtcg ccgccgagaa
     6301 taatacgctc acgagcaaat ccgactttat cggcaatagc aaaaacaaat tcgcgaaagt
     6361 ctgccggtgt cattccggta taaccgccaa attgattgac ctggtttgac gttgcttcaa
     6421 tcagcacttt acgcgtgctg ttgcgatcaa atgccagcgc cgcttcgata accaacggat
     6481 gtgcagaaca gactgaacat atgccaatat gttcaccagc tttatgccgg gcaattaacg
     6541 ttttcatgtt ttttccttgt taaatggcag gtgcgttatg ccctgccctc gcagccacaa
     6601 tcggcaatca ctttgctcac cacatcgcgc attgcagatt tagccgactg caaataatca
     6661 cggggatcgg tcgcttcggg gtgctcggtc aggtaatttt ttaacgcctg cgagaaggca
     6721 tttttcagct ccgttgcaac gttgattttg catatcccca gtttgatggt ttgctgaata
     6781 tccttagtcg ataatcctga cgcgccatgc agcaccagcg gtaaattcac ccactggcga
     6841 atgttctcca gtcgagaaaa atcaagcgcc ggtgctctgg catacatacc gtgagccgtg
     6901 ccgatggcga ccgccaggga atcaatcccg gttgcctcgg caaattcacg cgcctgaacg
     6961 gggttggtgt acaacgcatc tgcttcattg acttgcacat catcttcctg gccgccaagt
     7021 tgccccagct ccgcttcgac gctgacatca aagcgatggc aaaaatccac cacctctttg
     7081 acccgtgaaa tattttgcgc aaaaggcaaa tgcgaggcgt caatcatgac tgagcgcacg
     7141 ccagaacgaa ccttctgagc gatatcgtca aattttgtgt gatggtcgag atgaatcgcc
     7201 agtggatggt gatattgctt cgccatcgcg ctgaccagcg ccatcagatt ttctgtaccg
     7261 gcatgagtaa atgtgccggg cgttccggcg atgatgaccg gcgcatgcat gctggcagcg
     7321 gtttccacca ccacttgcat cgtttcgaga ttgtgaatat tgaatgccgg aaccgcataa
     7381 ccgccgcgct gcgcgttgtt cagcatctgc tttgtcgata ccacgtacat tttcacatcc
     7441 tgtcatttgt tttcgatttc aaaatataat gaaattattt gttttcaaat gtcgagataa
     7501 cgatcacaaa aacgacaatg tgaaaattaa tcagaatgac aggcaaaaaa aacggcctcc
     7561 cgggggaagc cgtaacgaag tgcgcgtgtt tttatgccgg atgcaacgta aacgcctttt
     7621 ccgtcctacg gggtggttct gttcgtatga ggcgcatcag gcgatagtaa ttttactatc
     7681 gagataaacg tcctgtacgg cgttaatcag tttcacgccg tcagccatcg atttcttgaa
     7741 cgccttacgt ccaagaatca gccccattcc gcctgcgcgt ttgttgataa ccgcagtacg
     7801 cactgcatcg ctgaggtcag tttcaccgcc tgcagcaccg ccggagttta tcaacccggc
     7861 ccggcccata tagcagttag ctaactgata acgcaccagg tcaatcgggt tttcactggt
     7921 cagtttgctg tagactcgat cgtcggtata accgtaatta atcgctttat agccgccgtt
     7981 attttccgcc attttttgtt tgacgatatc tgcacctatg gttgccgcca gatggttcgc
     8041 ctgaccggtc aggtcggcgg aaacatggta atcaacgcca tctttcttaa aggcggagtt
     8101 acgcaaatag gcccacagca ctgtcaccat gcccagctcg tgcgcacgtt caaaagccgc
     8161 agaactttct tcaatctggc gacgtgactc ttccgagcca aaatagatag tcgcaccaac
     8221 cgccaccgcg cccatgttga acgcctgctc cacgctggca tacagcgttt gatcgtaggt
     8281 attcgggtaa cttagcgtct cgttgtgatt aagtttgacg aggaatggaa tgcgatgcgc
     8341 gtaacgccgc gataccgatg ccagcacgcc gtaagttgac gccacacagt tacagcccgc
     8401 ttcgatcgcc agttcaacaa tgtttttcgg gtcaaagtag agcgggttag cggcaaatga
     8461 agctccggca gagtgctcaa cgccctggtc aaccggcaga atagaaagat atcctgtgcc
     8521 agccagacgc ccggtgttgt acaacgtctg catattacgt aacaccgctg gcgggcgatt
     8581 attgtcaatc atcacgcggt ctacgtagtc atgtccgggg agataaagct ggtcagaagg
     8641 aatagtcata caacggtgct gtaaaaggtt gtcggcgtct ttgccaagca actgcgcaat
     8701 atctgtcatt actatgctcc cgtaaattcc gattggatat cggctatgga ttgtcctggc
     8761 ccgccttttg cgggcaatca taatcctggt cgttacgggc gagtttttcc atcttttgga
     8821 tgttttttca gcgttttctg ttggctcgat tcatcagaaa aaatgttacc gcggtcaagt
     8881 tttcaccgca aaggtattta aaaggtatta ctaagtggta ttgtcatcgc gtaccttaca
     8941 ttacctgtca tgaaggaatt aaaagatgaa aacaacagca aagctgtcgt tcatgatgtt
     9001 tgttgaatgg tttatctggg gcgcgtggtt tgtgccattg tggttgtggt taagtaaaag
     9061 cggttttagt gccggagaaa ttggctggtc gtatgcctgt accgccattg cggcgatcct
     9121 gtcgccaatt ctggttggct ccatcactga ccgctttttc tcggcgcaga aagtgctggc
     9181 ggtattgatg ttcgctggcg cggtactgat gtatttcgct gcgcaacaga ccacttttgc
     9241 cgggttcttc ccgttactgc tggcctactc gctaacctat atgccgacca ttgcgctgac
     9301 taacagtatc gcttttgcca acgtgccgga tgtggagcgt gatttcccac gcattcgtgt
     9361 gatgggcact atcggctgga ttgcctctgg tctggcatgt ggattcttgc cgcaaatgct
     9421 gggttatgcc gatatctcac cgactaacat cccgctactg attaccgccg gaagttctgc
     9481 tctgctcggt gtgtttgcgt ttttcctgcc cgacacgccg ccaaaaagca ccggcaaaat
     9541 ggatattaaa gtcatgctcg gcctggatgc gctgatcctg ctgcgcgata aaaacttcct
     9601 cgtctttttc ttctgttcat tcctgtttgc gatgccactg gcgttctatt acatctttgc
     9661 caacggttat ctgaccgaag ttggcatgaa aaacgccact ggctggatga cgctcggcca
     9721 gttctctgaa atcttcttta tgctggcatt gccgtttttc actaaacgct ttggtatcaa
     9781 aaaggtattg ttgcttggtc tggtcaccgc tgcgatccgc tatggcttct ttatttacgg
     9841 tagtgcggat gaatatttca cctacgcgtt actgttcctc ggtattttgc tgcacggcgt
     9901 aagttacgat ttttactacg ttaccgctta catctatgtc gataaaaaag ctcccgtgca
     9961 tatgcgtacc gccgcgcagg ggctgatcac gctctgctgc cagggcttcg gcagtttgct
    10021 cggctatcgt cttggcggtg tgatgatgga aaagatgttc gcttatcagg aaccggtaaa
    10081 cggactgact ttcaactggg ccgggatgtg gactttcggc gcggtgatga ttgccattat
    10141 cgccgtgctg ttcatgattt ttttccgcga atccgacaac gaaattacgg ctatcaaggt
    10201 cgatgatcgc gatattgcgt tgacacaagg ggaagttaaa tgaaaacaga acgtattctc
    10261 ggtgctcttt atgggcaggc gttaggggat gcgatgggga tgccctccga gctttggccg
    10321 cgcagtcgcg tcaaagcaca ctttggctgg attgaccgtt ttctgcctgg gccaaaggag
    10381 aataacgcgg cctgttattt taaccgcgcc gaattcaccg acgatacctc gatggcgctg
    10441 tgtctggcgg atgcgttact ggaacgtgaa ggcaagatcg atccggatct gattgggcgt
    10501 aatattctcg gctgggcgct gcgtttcgac gcctttaaca aaaacgtact aggtccgacc
    10561 tcgaagattg cgcttaacgc cattcgcgac ggtaaacccg ttgctgaact ggaaaataac
    10621 ggcgtgacca acggcgcagc gatgcgcgtc tcgccattag gttgtttgct tccggcgcgc
    10681 gatgttgatt cctttattga tgatgtggcg ctggcctcca gcccgaccca taaatccgat
    10741 ctggcggttg cgggggcggt agtcatcgca tgggcgattt ctcgtgccat tgacggagaa
    10801 agctggtcag cgattgttga ttcactgcct tcaattgcgc gacatgctca acaaaaacgc
    10861 atcactacct tcagcgcctc actggcagca cgtctggaga ttgcgctgaa aattgtgcgc
    10921 aatgccgatg gcaccgaatc cgccagcgaa cagctttacc aggtcgttgg cgcaggtacc
    10981 agtactattg agtccgttcc gtgcgccatt gcgctggttg aactggcaca aaccgacccg
    11041 aaccgttgcg ccgtcctgtg cgctaacctt ggcggcgaca cagacaccat cggtgctatg
    11101 gcgacggcaa tttgcggcgc gttgcatggc gttaacgcta tcgatcctgc gttaaaggcg
    11161 gaactggatg cagtaaatca gcttgatttc aaccgctatg ccacagcgct ggcgaaatat
    11221 cgtcaacaac gggaggcgat atgagcggcg ctcgattaca cacgctgctg cctgaattaa
    11281 ccacgcgtca gccagtgatg gtcgtcggcg cggcggtcat tgatgtgatc gccgacgctt
    11341 acgctctgcc ctggcgtggg tgcgatatcg aactgaaaca gcagagcgtt aacgttggcg
    11401 gctgcgcact gaatattgcc gtggcgttaa agcgcctcgg catcgaagcg ggtaatgccc
    11461 tgccgctcgg tcagggcgtg tgggcggaga ttattcgcaa ccggatggcg aaagaggggt
    11521 taatcagcct gatcgataac gctgaaggcg ataacggctg gtgtctggcg ctggttgagc
    11581 cggatggcga acgcactttt atgtcattca gtggtgtgga aaatcagtgg aatcgcgaat
    11641 ggctggcgcg attaaccgtt gcgcctggca gcctgctcta tttttccggt tatcaactgg
    11701 cctcgccctg cggcgaattg ttagtggaat ggctggaaga gctgcaagac gtgacgccat
    11761 ttatcgattt tggcccacgt attggcgata tcccggatgc attactggcg cggatcatgg
    11821 cctgtcgacc tttagtgtca ctcaatcgtc aagaggctga gattgccgcc gaacgttttg
    11881 ctttatccgc agagataaca acacttggca agcaatggca ggagaaattt gccgcgccgt
    11941 tgatcgttcg tctcgataaa gagggcgcat ggtatttcag caacgacgct tctggctgca
    12001 ttccggcatt tccaacgcaa gttgtagaca ccattggggc gggcgacagt catgccggcg
    12061 gcgtacttgc cgggctggcc tctggtctgc cactggcgga tgccgtatta ctgggcaatg
    12121 cagtggcgtc gtgggttgtc gggcatcggg gcggtgattg tgcgccaacg cgcgaggaac
    12181 tactcctcgc acacaaaaac gtatagatcg ctgcgacagt ggctgatgct gtactcaata
    12241 ggccgttgct gttggtcaag cgccacttgc ttgatcacca gcaccggtat tttgctatct
    12301 aactgaatat gtgactgaaa ctcggcatcc ggcatccggg cgctaacgcg ggaacgcgta
    12361 cgttgtgggt aaatgtgctg gctgcggaag tagtcataaa gagaaatccc gatggcatca
    12421 acatcatgaa ttaaatgtgc cggaacccac gattcctcaa tcgataccgc ctcttcatcg
    12481 acatagcgaa tacgcttaag caaaaaaaca tcacttcctg cctcgaccgc cagctgctgc
    12541 gcgacgtctt cgggacattt cacgacacgc ttgttaaccc atagcgtatc gggctttttc
    12601 ccgcgcaaca ccacctgctg agaaaaacct cgcgcttctt tcaacgagta ttcgaagatg
    12661 ttgttgatct gcgtgccgta accacgcgaa cgcgtcacca cgccctcttc ttccagcgac
    12721 tgcatcgcct tgcgcaccgt aatgcgcgaa acgccggtta actgacttaa atcacgctca
    12781 ccgggcaaaa tattgccatg ctccagcacc ccgctgcgca cggcattttt taccgtttcg
    12841 gcaaacttaa tataaagcgg cgtgttatcc gccgctaaaa tacgctcgtt cagttgggca
    12901 atcagctggg tatgcgcttg ttccatttgt ctttttcctg ccggggaatc tggtgggagt
    12961 atatcactgc ttcttattta aggcgtttct ttaatcccat caacgaatcc ctgcaactcc
    13021 tcctctgtgc cattaaacac attaaaatcc accggaccat taatgccatc gacctgtcca
    13081 cggtcggaat gctgccagaa gcgccaggcc atgccgtcat tgtccgggcg acgttgataa
    13141 tagtgagcca cccaccacgg atattcattg aaatagcccg ccagattggt gtgataaaaa
    13201 acggctcctg agtaaataat cggctttttt cccgtacgtt tttcgaccat ttttagccac
    13261 tgacttaccc gcttgcgtaa ttcttttgcc gataattttc ctcgctcttc tacgtccagt
    13321 accgcaggga aatcgccttg tgagaaatcc accgtttgca gaaataatcg cgcctgaact
    13381 gaagcggata ctgacgggga gaaataatga tacgccccgc gcagcaggcc attttcccgg
    13441 cttagttgcc agttacgcga aaaatagggg tccaccagct tttcgccttc cgtcgcctta
    13501 ataaaagcaa actgtaagcg gataccgttg tcgcgcattt ttgccacccg ctgccagtcg
    13561 atccgctcct gccagcgcga aacatctatt ccgtgaatgg tgtaactggc aggaatgcga
    13621 atagcgaaag atttaaccgg gcgataaccg tagaagtgga tgtagtcttt aatctgaatt
    13681 gctgtcgaat agaaaaaatt caccgtctga cgcggataaa ttgcaacgat ggaaattagc
    13741 ccaagagcgc ataataaaac ggtgaatttt tttcgactgg tgattctcaa ctgcattggt
    13801 ttcatccctg aatgtcagca ccaaaggctg acaataacca aagcagctat agtacggtgc
    13861 tcaccaccag gcgtggaagt ggtgaaccgg accaataccg tgaccaactt ccagcgtgtc
    13921 ggcctgggct aatgccgatg aaagccagct ttttgcctcc tgtacggtgt cagcccagtt
    13981 tgtatggcgc gggcgcagtg cagccaacgc cgcagagagc gtacaaccag tgccgtgagt
    14041 gtttttggtc ataatgcgct gtgcggtaaa ccgttgttca ccctcgcggg taaacagcca
    14101 gtccgggctt tgctcatcat ccagatgacc acctttcatt agcactgcgc cacagcccat
    14161 cgccaacagc gatcgccctt gttccagcat ttcctgttcg gtgcgcgcgt gtggcgcgtc
    14221 gagcaaggcg gctgcttcgg gcaagtttgg cgttattaat gaaacctgtg gcaataatcg
    14281 actgcgtagc gtggcaactg ccgaaggtga aagcagcggg tcgccgcttt ttgccagcat
    14341 aacggtgtcg agtaccacgt tttggatctg ataacgttgc aaccgttctg ccaccgcttc
    14401 aacaatatcg gtttccgcca acataccgat tttagtggtg tcgattcgca catcgctgaa
    14461 caccgaatcg agctgggcgg cgacaaaatc aggctcaatg cgatacaccg actgtacacc
    14521 acgggtattt tgcgccacca gtgcagtaat aactgagcaa ccataagcgc caagtgccga
    14581 gaaggtttta agatcggcct gaatccccgc accaccactc ggatcagtac cggcaatcgt
    14641 cagagcgtta attcgtttca tgcctgcacc tcctgcgtca attgccagag cgcatcaagg
    14701 aaatgtggaa caaaactgcc tggcccctcg cttctggcga ctgcgcgttc tccggcttgt
    14761 ttcatccagt gacaggcaga tgcgatattt tccagcgtat cgcctggtag cgcacaacag
    14821 gcagcgacaa ccgccgatag tgcacagcca gttcctacca ctttggtcat taacggatcg
    14881 ccaccgtgaa tgccaatgat acgatgtcca tcggtaacat aatccacctc gccagtgacc
    14941 acgacgattg cgccagtttc ccgtgccagt gtttgtgcag caggtatcgc gttagctgcg
    15001 gcatcagtgg tatccactcc ccgtccacca ttagcaacgc cagctgatgc catgatttcc
    15061 gaagcattac cacgtatggc ggttggctta tgggacagaa gttccagaca aaaacggcgg
    15121 cgataatcga gcgcaccaac cgcaaccgga tcaagcgtcc agggcgtttg tgatcgtgtt
    15181 gcttgctcaa cggcagcact catcgcttgc gctcgtggcc gcgtcagcgt gccaacgtta
    15241 atcaacaagg cactggcgat agccgcaaac tgactggcct cttcggtttc gataaccatc
    15301 gctggcgatg cgccgagcgc cagcaaggta ttggcagtaa aagtttgcac cacatcgttg
    15361 gtcatgcagt gcacaagagg ggaatgttgg tgaaaaaggt gtaacgcgtg cgcagattgc
    15421 gctgaactca gcaggtcgac ttgcatagtt tgctcctgcc ataacgtgaa gaagcaatga
    15481 cctggtggtc cgtgacttcc ctacgctggc attatccaga tcaggtgata cgggtatttc
    15541 tcagccttca caaagaaggg caccccgagt cgtttggttg cgataataaa tcgcagagga
    15601 ggatggtaat gtccagcgca cgcgttgtaa acggggaaaa acggcggtat tacaataacg
    15661 ttgtgaaatt atttgatata agaatccagc accttcagaa cgacatccag atcttcttca
    15721 cgttttagct catccccctg gtgaacgatg tgttccgtca gatggccttt aatcacttcc
    15781 cgcatcagac cgtttaccgc gccacggata gcagcaatct gttgtaaaac tgcagcgcat
    15841 tcatgcggtt cgtcgagcat tttcttgagc gccacgacct ggccctgaat cttactggca
    15901 cgcgctttca gtttctgttt atcacggatt gtatgagaca tggcaacacc tggttaacaa
    15961 gaatatgaaa aatcatagca ctattaatct actggggggt agtatcaggt actggggggg
    16021 agtagaatca gattgccgaa ttaatactaa gaattattat catgaccgaa tttacaacac
    16081 ttcttcagca aggaaacgcc tggttcttca tccccagcgc catcttactt ggtgcgcttc
    16141 atggtctgga accggggcac tcaaaaacga tgatggcggc gtttatcatc gccatcaaag
    16201 gcaccattaa acaggcggtg atgctcggac tggcagcaac tatttcgcat accgcagtgg
    16261 tctggttaat tgcctttggc gggatggtga tcagcaaacg ctttactgct caatcagcag
    16321 aaccgtggct ccagttgatt tccgcagtga tcattattag caccgcgttc tggatgttct
    16381 ggcgtacctg gcgcggcgaa cgcaactggc tggagaatat gcacgagcat gatcatgagc
    16441 atcatcacca cgatcacgaa gatcaccacg accatggaca tcatcaccat cacgaacatg
    16501 gcgagtatca ggatgcccat gcacgagccc atgccaatga cattaaacga cgctttgatg
    16561 gtagagaggt caccaactgg caaattttgc tatttggctt aaccggtggc cttatcccct
    16621 gcccggcagc aattaccgtg ctgttgattt gcattcagtt gaaagccctg acactgggca
    16681 caacactggt cgtcagtttc agcctcggcc tggcgttaac gcttgtcacc gtaggcgttg
    16741 gcgcagcaat cagcgttcag caggtcgcaa aacgctggag cggatttaac actctcgcta
    16801 aacgcgctcc ctatttttcc agtctgttga ttggcttagt tggtgtgtat atgggcgtac
    16861 atggcttcat gggcataatg cgataactca atcctttagg cttgcatgtt atcttcagcc
    16921 caggatgatt cttaaatcag ctatttccgc tgactctctg ctcgctaagg atgcttttag
    16981 ggcatccttt catttacact ttttacgaaa tcatgggatc actaacaaaa tatcgcttgt
    17041 cagttatatt gtatggcagg aaagatatgc gactgatatt acagatcccc aaagtggaga
    17101 gtttatgacc attaaaaata agatgttgct gggtgcgctt ttgctggtta ccagtgccgc
    17161 ctgggccgca ccagccaccg cgggttcgac caatacctcg ggaatttcta agtatgagtt
    17221 aagcagtttc attgctgact ttaagcattt caaaccaggt gacaccgtcc cagagatgta
    17281 ccgcaccgat gagtacaaca ttaagcagtg gcagttacgt aacctgcccg cgcctgatgc
    17341 cgggacgcac tggacctata tgggtggcgc gtacgtgttg atcagcgaca ccgacggtaa
    17401 aatcattaaa gcctacgacg gtgagatttt ttatcatcgc taaaaaaaag ccccctcgga
    17461 gagagggggg aatgcagaca ccttaatatt tttattatta gccacttgct cgtcttgctt
    17521 gttattagtc gtatttcacg ttgattaatg cggttgcctc cagtgcgcca gatttaactt
    17581 tgtttgtatc gtagacgtag taactggccg ttatcggaat tgaaaccgtt gtatctgtaa
//
LOCUS       DACADF010000096        17581 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.098, whole genome shotgun sequence.
ACCESSION   DACADF010000096 DACADF010000000
VERSION     DACADF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.098"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(115..1143)
                     /gene="epmB"
                     /locus_tag="ISB30_003135"
     CDS             complement(115..1143)
                     /gene="epmB"
                     /locus_tag="ISB30_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAP1990740.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1185..1751
                     /gene="efp"
                     /locus_tag="ISB30_003136"
     CDS             1185..1751
                     /gene="efp"
                     /locus_tag="ISB30_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP1990741.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1803..1928
                     /gene="ecnA"
                     /locus_tag="ISB30_003137"
     CDS             1803..1928
                     /gene="ecnA"
                     /locus_tag="ISB30_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAP1990742.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            2039..2185
                     /gene="ecnB"
                     /locus_tag="ISB30_003138"
     CDS             2039..2185
                     /gene="ecnB"
                     /locus_tag="ISB30_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAP1990743.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2361..2678
                     /locus_tag="ISB30_003139"
     CDS             2361..2678
                     /locus_tag="ISB30_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAP1990744.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2675..3208)
                     /locus_tag="ISB30_003140"
     CDS             complement(2675..3208)
                     /locus_tag="ISB30_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAP1990745.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3297..4430)
                     /gene="blaEC"
                     /locus_tag="ISB30_003141"
     CDS             complement(3297..4430)
                     /gene="blaEC"
                     /locus_tag="ISB30_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAP1990746.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQRIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            complement(4493..4852)
                     /gene="frdD"
                     /locus_tag="ISB30_003142"
     CDS             complement(4493..4852)
                     /gene="frdD"
                     /locus_tag="ISB30_003142"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAP1990747.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4863..5258)
                     /gene="frdC"
                     /locus_tag="ISB30_003143"
     CDS             complement(4863..5258)
                     /gene="frdC"
                     /locus_tag="ISB30_003143"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAP1990748.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5269..6003)
                     /locus_tag="ISB30_003144"
     CDS             complement(5269..6003)
                     /locus_tag="ISB30_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAP1990749.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(5996..7804)
                     /gene="frdA"
                     /locus_tag="ISB30_003145"
     CDS             complement(5996..7804)
                     /gene="frdA"
                     /locus_tag="ISB30_003145"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAP1990750.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8129..9106
                     /gene="epmA"
                     /locus_tag="ISB30_003146"
     CDS             8129..9106
                     /gene="epmA"
                     /locus_tag="ISB30_003146"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAP1990751.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9325..10827
                     /gene="yjeM"
                     /locus_tag="ISB30_003147"
     CDS             9325..10827
                     /gene="yjeM"
                     /locus_tag="ISB30_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAP1990752.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAISFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVVKNPQWVAE"
     gene            10925..11239
                     /locus_tag="ISB30_003148"
     CDS             10925..11239
                     /locus_tag="ISB30_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HAP1990753.1"
                     /translation="MSARMSVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(11270..14593)
                     /gene="mscM"
                     /locus_tag="ISB30_003149"
     CDS             complement(11270..14593)
                     /gene="mscM"
                     /locus_tag="ISB30_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAP1990754.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPANANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14615..15583)
                     /gene="psd"
                     /locus_tag="ISB30_003150"
     CDS             complement(14615..15583)
                     /gene="psd"
                     /locus_tag="ISB30_003150"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAP1990755.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVDLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15680..16732)
                     /gene="rsgA"
                     /locus_tag="ISB30_003151"
     CDS             complement(15680..16732)
                     /gene="rsgA"
                     /locus_tag="ISB30_003151"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAP1990756.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16827..17372
                     /gene="orn"
                     /locus_tag="ISB30_003152"
     CDS             16827..17372
                     /gene="orn"
                     /locus_tag="ISB30_003152"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAP1990757.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         4039 a         4507 c         4628 g         4407 t
ORIGIN      
        1 cagaaaagaa aggttaatta ttggtgtcgg ttatataaat agtaataaca gcaatgtata
       61 tgaataatta aaggttatgc aacgggcaaa gattaaactt ccctgtttaa tcagttactg
      121 ctggcgtagc tggagatcca gcggtgtttt gctgggttcg ccgccaattt ctcgcgccag
      181 tttaggcacc agatatcccg acaccagtgt cagcaactca cgcataatct gccgtgcttc
      241 gtcatcactc accataaaat gcgccgcgcc ctgtacttta tcgagcacat gcaggtaata
      301 cggcatcaca cccgcatcga acaacgcatt actcaggttt gccagcgttt gcgcgttatc
      361 gttcacacca cgtaacagaa cgctctggtt cagcaaagta acgcctaccc ggcgcaactt
      421 agccatcgcc tgacggaatg tttcatctac ctcattggca tggttaatgt gattcaccag
      481 caagatttgc agcgtagaat gagaaaagcg ttcaaccagc gcatctgtga tgcgtgccgg
      541 gatcacaatc ggcagacggc tgtgaatccg cagacgtttg atatgcggga tggcttccag
      601 ttgtgtgagc aaccagtcca gctcgtgatc tttcgccatc agcggatcgc cgccggagaa
      661 aatcatctcg tccagttccg gatgcgcagc aacatactca agtgcagtct gccagttacg
      721 cttgttgccc tgattttcgg cataggggaa gtggcgacgg aagcaatagc ggcaatttac
      781 cgcgcagccg cctttgacca gcaaaagcgc ccggttgtgg tatttatgca acaaacctgg
      841 cactacgctg tgctgttctt ccagcgggtc ggtggagaat ccgggcgcga caacaaactc
      901 atcttgcgag gtaattacct gacgcaaaag cggatcgttc ggattgcctt tctccatgcg
      961 atcgataaat gagcggggca cacgcagggc aaacagcttt ttggcgctgc gtccggccaa
     1021 cagtttttcg tccgcgtcta tattcaaaag acgcagaagt tcatcaggat cggtcacaac
     1081 atcggcaagt tgcgttaacc aatcttctct ggatggggta tttagggtta caatatgcgc
     1141 cattttgtgg cttagctacc aattaacaaa tttcagaggg ccttatggca acgtactata
     1201 gcaacgattt tcgtgctggt cttaaaatca tgttagacgg cgaaccttac gcggttgaag
     1261 cgagtgaatt cgtaaaaccg ggtaaaggcc aggcatttgc tcgcgttaaa ctgcgtcgtc
     1321 tgctgaccgg tactcgcgta gaaaaaacct tcaaatctac tgattccgct gaaggtgctg
     1381 atgttgtcga tatgaacctg acttacctgt acaacgacgg tgagttctgg cacttcatga
     1441 acaacgaaac tttcgagcag ctgtctgctg atgcaaaagc aattggcgac aacgccaaat
     1501 ggttgctgga tcaggcagag tgtatcgtaa ctctgtggaa tggtcagccg atctccgtta
     1561 ctccgccgaa cttcgttgaa ctggaaatcg tggataccga tccgggcctg aaaggtgata
     1621 ccgcaggtac tggcggcaaa ccggctaccc tgtctactgg cgctgtggtt aaagttccgc
     1681 tgtttgtaca aatcggcgaa gtcatcaaag tggatacccg ctctggtgaa tacgtctctc
     1741 gcgtgaagta atgcggttgt ggtgcggcct gcaggctgca ccatcactta ttcaggttag
     1801 agatgatgaa acgccttatc gttcttgttt tgcttgccag cacgctgctt acgggctgta
     1861 acaccgctcg aggtttcggc gaagatatca aacacctcgg caactccatc tcccgcgctg
     1921 ccagctaatt tttcttctct tccgaaaaat catcagattt ccgtcatttt tggcgatgtt
     1981 gtctattatt aatttgctat aggcaaacat aaataacatt acctaaaagg aagacgttat
     2041 ggtgaagaag acaattgcag cgatcttttc tgttctggtg ctttcaacag tattaactgc
     2101 ctgcaacacc acgcgtggcg ttggtgaaga catttctgat ggcggtaacg cgatttctgg
     2161 cgcagcaacg aaagcgcagc aataagcaat aacggtacga cagctgtgtc gtgccgtttg
     2221 ttttttctgc gatagttaca taggtaatag ttgaaattcc cctgccacct ggcaaaatat
     2281 ccgttcaacc atcagctttg caggacgacc tgcaaacgcg tcttttcacc ggggacggcc
     2341 ccaattttcc ggagcctgat atgtcctgga ttatcttagt tattgctggt ctgctggaag
     2401 tggtatgggc cgttggcctg aaatataccc acggctttag tcgtttgacg ccaagtgtta
     2461 ttaccgtgac ggcgatgatt gtcagtatgg cgctacttgc ctgggcgatg aaatcgttac
     2521 cagtagggac ggcttatgcc gtgtggacgg gtattggcgc agtcggcgcg gccatcaccg
     2581 gcattgtgtt gctcggtgag tccgctaacc cgatgcgtct ggcgagtctg gcgttaatcg
     2641 tattggggat tattggtctg aaactcagca ctcattaact accgggctgc tgtacccaaa
     2701 taaacttact gacatcaaac ccttcccggg tcgcgactgc cagcatctcc tgtttcactt
     2761 cgtcagaaat ggttggcgtg cgggagagta tccacaggta gtcgcggtcc gggccgcaaa
     2821 ccagcgcatg gcggtattcc cgatcgagtg caataacgtt ataaccgcca tagaaaggac
     2881 caaagaatga cactttcagc gcagcgcgag ttggtgcgcc ggtaaagtac gctttccctt
     2941 cactctgctg ccacatttct ctgtcagggt tatagccttt attaatgaca ttcaggccgc
     3001 cgtcatcacg caggctgtat gttgcggtga ctttttccag tccacgttca aagcggtgat
     3061 caaaacgggc aatctcatac caggtgccga gatagcgttt agcgtcgaaa ttattcacta
     3121 cggtcacgcc acgcggcggt gtaggagaac tgcaggcaac gaccagaaat gcagctgtcg
     3181 ctgcggcaac gagagggagc aggcgcataa atgtttcctt actggttttt ttctaagtgt
     3241 agatgacagc aaggaaaagc ggagaaaagg tccgaaaatt cggacccgat gaaattttac
     3301 tgtagagtgt tgagaatctg ccaggcggcg gtaactctcg ctggattggg atagtttttg
     3361 ttagccagca tcacgatacc cagctctttt tctggaataa acgcgacata gctaccaaat
     3421 ccgccggtcg cccccgtttt atgtacccat gatgcgcgta ctgcaggagt tgggggcgta
     3481 atcggtttta cggggcgtgc tgccagtgca attttattgt cactgccgtt aatgatgatg
     3541 tcaggattta ccggccagtc cagcatttcc cagcccagac cctgatacat atcgccggtt
     3601 tgccagtagc gagattgtgc cagttgtatc ccttgttgaa gtattttctc attgatatca
     3661 aggggtttta aattgctttg cacccagcgg gccatatctt caatggtcga cttcacacca
     3721 taagcttcag catctaacgc ccctggcgaa acatgtactg ccttaccttc gcgatatccc
     3781 caggcgtagt tcttttcttc tgcggacggt acattaatcc acgtatgatt gagtttgagt
     3841 ggctggaaga cacgagtttt catcgcctgc tcaaaactca aaccagacgg cttcacagcc
     3901 agtgcgccga acaaaccgat actggagttg gcatacagac gttgtgttcc tggagcccat
     3961 gcaggctgcc agttttgata gaagcgcagc aagtcgcttg aggatttcac ctcatccggc
     4021 acctgcaatg gcaggccgcc agcggtgtag gttgcgagat gtaatagtgt gatcccattc
     4081 cactgtttag cggtaagttc aggccagtat tttgttgtgg gatcgcttaa cttgatttcc
     4141 cctcgagcaa tagcgtcgcc accaagcacg cccgtaaatg ttttgctgac cgaacctaac
     4201 tcaaacaacg tttgctgtgt gacgggctgc tttttggcga tgtccgcata gccccaggta
     4261 aagtaataag gtttaccctg ataaattacc gctaccgcca taccggggat cctttgttgc
     4321 tctataagcg gggtaattgt gcgatgcaca atatcgttga tttgttgagg ggcggcaaat
     4381 gtggagcaag aggcggtaat taataaggtg cagagcgtcg ttttgaacat agggtctggt
     4441 ttccatacaa aacggcccgc cataggcggg ccggatttac attggcgatg cgttagattg
     4501 taacgacacc aatcagtgtg acaactgtca ggatagcagc cagaccgtag aaaacccatt
     4561 tacccgcagg tacgtggatt ttcagatcgt gcatcgcgtg gtgcattcgg tgtaaaccac
     4621 accacagcgg cagaacgatc atcaggaaca ggaatacgcg accaatgaag ctctgcgcga
     4681 acgccagaac gcgctcgtag ctcagcgcat cacctggaaa cagccctagc ggtagcagaa
     4741 taccaaccag taggatcatc accggcgcaa tgatggcgct ccacatacca ccggccccga
     4801 agaggcccca gaataccggt tcatcagaac gctttggatt tggattaatc atctcaggct
     4861 ccttaccagt acagggcaac aaacaggatt acgatggtgg caaccacagt taccgcccag
     4921 agacttttga taattggctc tggtcccatt ttttcgtctt ttacaatgat attggccgct
     4981 tttggtgcca gctcaaacca ggttttggtg tgcagtaggg ctgccgccag agtgatcagg
     5041 ttaatgatca cgataaccgg gttttgtaaa aagtcgacga atcccgccca ggcttccggg
     5101 ccatttttca gggcaaacag cccgaaaatc agttcaatgc tgaaccacac agccggaact
     5161 gccgtgcctt cgcgcagcat gtaaaagcga taaaacggca attttttcca ccaggtggac
     5221 gtcattggcc gtacatacgg tttacgttta gtcgtcatgt tgcactcctt agcgtggttt
     5281 cagggtcgcg ataagaaagt ctttcgaact ttctactttg ccctgctgaa tggccgcagc
     5341 cggatcgacg tgtttcgggc agacttcgga gcagtagccc acgaaagtac agctccatac
     5401 gccgttctgg ctgttcaact gcgccatacg ctccttctta ccgtggtcgc ggctatcttc
     5461 gttataacga tgcgccagcg taatggcagc cggaccgatg aactctgggt tcaggccaaa
     5521 ctgcgggcac gcggcgtagc acagaccaca gttgatgcaa ccggagaact ggtgatactt
     5581 cgccatctgc gccggggtct ggatgttagt accctgatcc gcggtgcggg agttgccgat
     5641 gatgtacggt ttgatcgctt ccagactttc gatgaagtgg gtcatatcga ccaccagatc
     5701 gcgttcaatc gggaagttag ctaacgcttc aaccttcata ccgtcggtgt aatcacgcag
     5761 gaaggtttta catgccagtt ttggcacgtt gttaaccatc atgccgcagg agccacaaat
     5821 cgccatacgg caggaccagc ggtagctcag gtccggtgcc aggttgtctt tgatgtagcc
     5881 cagcgcatcc agtaatgagg tagttgcgtc ataaggcact tcatagaatg cgctatgcgg
     5941 tgcggtatcg acttccgggt tatagcgcac cacctcaatt ttcaggtttt tcatctcagc
     6001 cattcgcctt ctccttctta ttggctgctt ccgccttatc ggctgcatcc gcttcgccac
     6061 cgtaaacacg tttagctggc ggcagcgtag taatcttcac gtcgctgtac tccaggcgag
     6121 tcgtgccatc agcatcgcgg aaggcgaggg tgtgtttgag gaagttgacg tcgtcacgct
     6181 cggtgcaacc ttcgtccaga cgctggtgcg cgccgcggga ctctttacgt gccattgcgg
     6241 agtgcgccat acattcagca acgttcagac cgtggcccag ctcaatggtg tagagcaggt
     6301 cggtgttgaa cacgctggaa gtgtcggtga tgcgcacgcg cttgaagcgt tcctgcagct
     6361 ctgccagctt gtcgatggtt ttctgcatca gttccggcgt acggtagata ccgcagcctt
     6421 cttccatggc catgcccatt tcgtcgcgga tcttagccca gttttcgccg ccatcctggt
     6481 taaccagatc tttcagacgt tgttcaacgc cagctgcctg cgcttcaatt gccgcttcgt
     6541 tgccattacc ggcagttgct gcacgctctg tcgcttgttc accggccaga cgaccgaaga
     6601 ccaccagttc cgccagcgag ttggagccca gacggttcgc accgtgcaga ccaacagagg
     6661 aacattcacc cacggcgaac agacctttaa tgcgggtttc acagttctga tcggtttcga
     6721 taccgcccat ggtgtagtgt gcggtcggac gtaccggaat cggttcttta accggatcga
     6781 cgccaacgta cgctttcgcc agttcgcaga tgaacggcaa acgttcatgc agttttttct
     6841 cgccaaggtg acgcaggtcg agataaacca catcgccacg cggcgtggag atggtgttgc
     6901 ctttacgcca ttcgtgccag aaggcctgag aaactttgtc gcgtggaccc agttccatat
     6961 atttgttttt cggctcgccc agcggagttt ccgggcccat gccgtaatct tgcagataac
     7021 ggtagccatt tttgttgacc agaataccgc cttcaccgcg gcagccttcg gtcatcagga
     7081 taccggaacc tggtagaccg gttgggtgat actgaacgaa ttccatgtca cgcagcggaa
     7141 cgccgtggct tagcgccata cccataccgt caccggtaac gatgccgccg ttggtgttgt
     7201 aacgataaac gcgacctgca ccgccggtag ccataacgac cgcgttagca cggatctgca
     7261 ccagcgtgcc ttccatcatg ttcattgcta ccaggccgcg aacatgacca tcatcaacta
     7321 gaatatccag cacgaaatgt tcgtcgaaac gctggatctg cgggaattgc agagaggtct
     7381 ggaacagcgt gtgcagcata tggaagccgg tcttatcggc ggcgaaccag gtgcgctcga
     7441 ttttcatgcc gccgaagcga cgtacgttga cgctaccatc cgggcgacgg ctccatgggc
     7501 acccccacag ttccagttgg gtcatttcgg ttgggcagtg gtggacgaaa taatccacga
     7561 catcctgctc acacaaccag tcgccacccg ctactgtatc gtgaaagtga tattcgaagc
     7621 tgtcatgatc ctgcgcgaca gcggcggagc ccccttctgc agcaacggta tggctacgca
     7681 tcgggtatac ttttgagatt agtgcgattt ttgcatttgg atttgcctgc gcggcagcaa
     7741 ttgcagcacg taatcccgcg ccaccggcgc ctacaatggc aagatcggct tgaaaggttt
     7801 gcacgacatt cctccagatt gtttttatcc cacagccccg tacttcaggg taagtgcctg
     7861 aaagttacgg tctgcgaacg ctattccact gctcctttat aggtacaaca gtatagtctg
     7921 atggataagt ctgaaatttg acgagatcga tttttttagt gcgcaagagg gctaaattat
     7981 cactgaagat gattaattta attactaaac catcagatcg tgcttttttt agtcactgcc
     8041 caccgttgtg ttattcctgc gtaaaatttg tctcgccgtc gcgttgcgag tagacttcgt
     8101 gcccttgtca aaaactggag atttaactat gagcgaaacg gcatcctggc agccgagcgc
     8161 atccattcct aacttattaa aacgcgcggc gattatggcg gagatccgtc gtttctttgc
     8221 cgatcgtgga gtgctggagg tggagacgcc ttgtatgagc caggcgacgg taaccgatat
     8281 tcatttggtc ccgtttgaga cacgtttcgt tggccccggg cattcgcagg ggatgaatct
     8341 ctggttaatg accagcccgg aataccatat gaagcgcctg ctggttgccg gttgtgggcc
     8401 ggtattccag ctgtgccgca gcttccgtaa tgaagagatg gggcgttatc acaaccctga
     8461 gttcactatg cttgagtggt atcgaccgca ctatgatatg taccggttga tgaacgaggt
     8521 ggacgatctc ttgcaacagg tgctggactg tccggcagca gaaagccttt cttatcaaca
     8581 agctttcttg cgttatctgg aaattgaccc actctctgcc gacaaaacgc aactgcggga
     8641 agtggcagcg aaactggatt tgagcaacgt tgccgatact gaagaagacc gcgacacgct
     8701 tctacaattg ctgtttacct ttggcgtaga gccaaatatt ggcaaagaaa aaccgacctt
     8761 tgtgtaccac tttccagcca gccaggcatc actggcgcaa atcagtaccg aagatcatcg
     8821 ggtcgctgaa cgctttgagg tttattataa aggtattgag ctggcgaatg gtttccatga
     8881 attgacggat gcccgtgagc agcaacaacg ctttgaacaa gataaccgta agcgcgcggc
     8941 gcgcggtttg ccgcagcacc ccattgacca gaatctgatt gaagccttga aagtcggtat
     9001 gcctgactgt tccggcgtgg cattaggtgt tgatcgtctg gtgatgttgg cgctgggcgc
     9061 ggagacactg gctgaagtca tcgcctttag cgttgaccgg gcataattct gaaaaattac
     9121 tgaattaaca gcgaagaatg gcgtgataag cgccattctg tagcatatgt tttcattttt
     9181 ccgctaaagg ccacaattta agactggata tttgatatca tccaggtatc aattctgtat
     9241 tggtttttac gctgcccgct caatgttggg cagtgcagtt cagtgggcgc gttttataca
     9301 cgcgctgaaa tgaaggatgg tttcatgcct cactcgataa aaaagatgag tctgatagga
     9361 ctcatattga tgatctttac ttctgtattt ggatttgcca atagcccatc ggcttattac
     9421 ttaatgggtt atagtgcgat ttccttttat atattttctg cattgttatt ctttattcca
     9481 ttcgccttaa tgatggctga aatgggagct gcttatcgca aagaagaagg cggtatctat
     9541 tcctggatga ataatagtgt cggaccacgt tttgccttca ttggtacgtt tatgtggttt
     9601 tcctcttata tcatctggat ggtgagtacc tccgcgaaag tttgggtacc gttctcaaca
     9661 ttcctctacg gtagcgacat gacccagcac tggcatattg ccggactgga gcctacgcag
     9721 gtggttggtc tgctggcagt ggcatggatg attctggtca ccgtcgttgc ttcaaaaggg
     9781 attaataaaa ttgcccgcat tactgcggtg ggcggtattg cagtaatgtg tctgaattta
     9841 gtattgctgt tagtaagcat tactattttg ttattaaatg gtgggcattt cgcgcaggat
     9901 attaatttcc ttgcatcacc gaacccaggt tatcagtccg gtctggcaat gctatcgttt
     9961 gtggtatttg ccatttttgc ctatggcgga attgaagcgg ttggtggtct ggttgataaa
    10021 acggaaaatc cagaaaagaa ctttgccaaa ggtattgttt ttgccgctat tgttatttca
    10081 atcggttatt cgctggcaat atttttatgg ggcgtcagca caaactggca gcaggtatta
    10141 agtaatggtt ccgttaacct cggcaatatt acctatgtgc tgatgaagag cctcggggtg
    10201 acgctgggta acgcactgca tttgtcacct gaagcgtcat tgtcgctggg cgtatggttt
    10261 gcgcgtatta ccggactgtc gatgttcctc gcttataccg gtgcgttctt tacgctttgc
    10321 tattcaccgc tgaaagccat catccagggg acgccgaaag cattgtggcc ggaaccgatg
    10381 acgcgcctga atgcgatggg gatgccgtct atcgccatgt ggatgcagtg tgggttggtt
    10441 actgtcttca tcctgctggt ttcgtttggt ggcggtaccg catcggcgtt ctttaacaag
    10501 ctgacgctga tggcgaacgt gtctatgacg cttccttacc tgttcctcgc gctggctttc
    10561 ccgttcttta aagcacgtca ggatctcgac agaccgtttg tgattttcaa aacgcgtatg
    10621 tcggcaatga ttgcgacagt ggttgtcgta ctggtggtga catttgcgaa cgtcttcacc
    10681 atcattcaac ctgtggttga agccggagac tgggacagca cattgtggat gattggcggc
    10741 cctgtcttct tctcgctgtt agcgatggcg atttaccaga actattgcag cagagtggta
    10801 aaaaatccgc agtgggtggc tgagtaatca aatgccctat cgtcggcctg acgatagggc
    10861 gtaatgacag ggaattagtg agatttcgta tgcgctatag cgaaattaaa ctatgggtta
    10921 cctcatgagt gcgcgcatgt ctgtcttgtg ctgcatctgg ttcattgtgg cgttcctctg
    10981 gataaccatt acctccgcgc tggacaaaga gtggatgatt gatggacgcg ggattaacaa
    11041 cgtctgtgat gttttgatgt atctcgaaga tgacgatact cgcgatgtcg gtgtgataat
    11101 gacgttaccg ctctttttcc cttttctctg gttcgctttg tggcgaaaaa aacgcggctg
    11161 gtttatgtac gccaccgcgc tggctatttt cggctactgg ttgtggcagt tttttctgcg
    11221 ctatcagttt tgcttgtgag ccggatgggt tcatccggcc acaaagacct tacaaacttc
    11281 ccgcctgacg acctttgccc gcagaagtta atgttctccc cgtttgttta ccattcaggc
    11341 tttccagacg catctggaag ggcgggaatg gcatatcgat accgtgggcg tggaagccag
    11401 ccagaatcag ctggtgaatc tcatggcgta gcggcatacg gtgacccatc tcagcggcgt
    11461 aaatacgcag ctcgaaaatc tgaatcccct gctgcagatc caccaggaag acttccggcg
    11521 ccgggttgtc gatcaccagc gagcagcgac gcgctgcggt gagcaggatt tccgtcactt
    11581 cttcactgtt ggcattggca ggggccggaa tcgtcaacac cacgcgcgtg accgagtcag
    11641 agagcgacca gttgataaac tgctcggtaa taaacgcctt gttcggcacg ataatttctt
    11701 tgcggtccca gtcgctgatg gtggtggcgc gggtgttaat tttcgtcaca ctaccggtaa
    11761 gatcgcggat cgttaccgta tcgccaatgc gaatcggttt ttcgaacagg ataatcaggc
    11821 cagagataaa gttggcgaaa atttcctgca aaccaaaacc gagaccaaca ccgagcgcgg
    11881 cgaccagcca ctgcaatttc gaccactcaa taccaatcat cgagaagccg accagcccgc
    11941 caattagcat cagcagatat ttggtgatgg tggtgatggc gtagcccgta cccggcgtta
    12001 aatccaggtg ctgcagaatc gccagttcca gcagcgcggg caggttgcgc accagctgcg
    12061 tggtgatgat aaacaccaga atggcaatca gcaccgcacc gagggtaatc ggctccaggc
    12121 tttctacgcc ctgtaccgtg gaagtgacat cccacagcga aatattttcg aggaagccga
    12181 aagcggaatg gatttctgac cacagcacaa tgaccgaaag cagggcgatc aacatcaaaa
    12241 ttgagcgcac cagccgcaag gattgcgcac tgatggcatc gagatcgact tcgctttcat
    12301 cgacttcaat tgctccttcc gggctactgt gatgatgcgc ttcctcttcg ccacgcgcac
    12361 gctgcgctaa catctctgcc cggcgatgct ttgcccggtc aaacgccagc ctgcggcgct
    12421 ggatcagcat ccagcggcgg ataacgtgat aaaccaccag tagcaggaac cagatggcaa
    12481 ccgaggtttc aagcctcgct aacagcgcct gcgccgttgc cagataaccc accgccgacg
    12541 ccagaatggc aaccagaggt gcgccaatca tcatgttcca cagcatatgg ttggtaatgt
    12601 tgtcgccgct gccttctttg ttgagataca gcgggatccc ggcctttttc aggctgaggg
    12661 tgaccaccgc cagcgcaccg caaatgagga taaagcaaag ccgtcccagc gaaccggaaa
    12721 attcgcggtc gtcgaggtta tcgaacatca tcagcgccat aatcagcggc acaataagcc
    12781 cgatgctcat caggtagtaa cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat
    12841 gagcgataaa caagccgttc gggcgagcaa aggtggcgca aatcatcact acccacagca
    12901 gcggcacggt ggctgttacg ccatcaccaa tcgcgaccgc cagcggataa ggccacgcct
    12961 cgcgcaagcc gtaacccagc gtcatccaca gcacaggcag cggtgacgcg acgagaatcg
    13021 accagaaaag agtgcgtaac gtcagccaga agtgatcctg agtcactttg ccgactttcg
    13081 ccgccgaacg ttcaagaaaa cgggtgaaat agcggcgcga gtaaatactg caaccgacca
    13141 gaatcaacgc gccaaacagc ggcaaaatcg tctctttact ggtcagcatc atcacactgg
    13201 ctttgcccaa ctgactgaag gtgtccagcg aaatgagacg acgcagatcc tgggcgattt
    13261 ccagcggcca ggcgatggtc atcgggcgca cgtcagaggt ccagaacaga tagcggtgcg
    13321 tcgcttcgtt cacctctttc agtgcatcct ccagttgccc gttggagact ttcagcttgg
    13381 tcagctccag cagtagcgtg tcgccaccct gcaacaatga gttcagcaac tcacgctgag
    13441 tgcgtagctg tgcttccaga atacggtttt gctcagcggt cagcggctga ccgtcggcct
    13501 gatggatttg ccgtagcagc ggctgtttat taagcagatc ctcataacgt aaccgttgca
    13561 cacgtaactg cgccatttcg gtatcaagct gttgtggttt tggcatttcc ggtagccgtg
    13621 ccacctgcgc ccgcaacgct tcgccgagca ggttagatga tcccagccat tgcgactgtt
    13681 cacgcagcgt attcaacgcc tgccggacct gtaacgtctg gctggcagcc tgacgctgtt
    13741 gcgaggcaac gagatccatc cgctgcgcct gttgattcag tgccgccgat agttcgcggt
    13801 taattttaaa ctgggcgacg atatcttttg gcaaatcggc gctactttcc gccagttgct
    13861 cggtactttc cagcgcccgt tccgcttcca gttggcgttg actattcaac tggttgcgta
    13921 gtgcttgcaa atatgcatcc agctgctggc tctctttttc ggccagctct gagcgcaagc
    13981 gcgctaattc ctggcggtta ttggcagata gctgcgccag ctccagttca tcaacgagcg
    14041 ctttcagacg tgcggagtca gactgcaacg cgaaattttg tgcctgattg agcggggtat
    14101 tgccggtaag cgtccccagg cggcgctcga tttcattcaa ctgacggcgg gcatcggttt
    14161 gctgttgcgg cagttgattc agggaatcgg caatctcgcg ggcgcgctcc tgctcttgct
    14221 gggcctgacg gcttttatcc agcaactgac tgctgatctg gagaatttcc tgattcagcg
    14281 catcgttaga cattcccggc gacacgctgc gtggctcgtc acgcatgttg tttaactgcg
    14341 cgcgcagagt ggcggaaagt ttcgggtaat tatcaatgac ttcttgatat tgcttgatgc
    14401 gctcaaggga accttttcgt tcctcaagcg catttaaggc agactggagc gcctctacga
    14461 cttccggctg cgcaggtttc gccgcttttg cctgctccag ttcctgggtg atttgtttgc
    14521 tatcgggggc cgtcgcggcg tacgcccccc aactgaggca ccaggccatc agaaaagtga
    14581 taatcaggcg cacgtcagcg tttcctttga tggattagac ctggtctttt ttatcgtcaa
    14641 ccaatgggct ggcgtcgtgt tctgcttcga tctcttcagc aggaagcggg gctggttcag
    14701 catctggcgt aacaaaggtt tcggtagata ctgccagcgg ctggccaatt ttcgtgacag
    14761 acaggctctc cagttgctca accaggtcca ctttacctgg tgcaaacagg ttgataacgg
    14821 tagagcctag tttaaagcga cccatttcct ggcctttcag cagtgccaca gaaccgtcgt
    14881 tttccccggc aggccaggtc cagcgcttga tgatgccttc gcgcggcggc gtaatggtgc
    14941 ccgcccatac cgtctcaatg ctgcctacaa tcgtcgcacc gaccagaatc tgcgccatcg
    15001 ggccaaattc ggtatcgaac aggcagatca cgcgttcgtt acgggcaaac agattcggca
    15061 cgttctgagc cgtgagatgg ttaacggaga agagatcgcc cggcacgtag atcatttcac
    15121 gcagaatacc gttgcacggc atgtgtacgc ggtggtagtc acgtggggag aggtaagtgg
    15181 tcacaaacgt accgttacgg aagagatccg ccatcagata gttgcctgcc agcagggctt
    15241 cgaggctgta gttgtggcct ttggcttgca ggattttatc ttcttcgatt ttacccaact
    15301 ggctgataac gccatcggca ggcataacca ggacattcgg atcggtatcg attgggcgta
    15361 cttcatcacg cagcggacgg acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg
    15421 gcttttgcgc ctctttcatg tcgaccttgt agtatttaac gaacagatcg ataaccagtt
    15481 ttgtcagcca tcctgcccgc ttgcttgcgc cccaacccgc caggcgagta agccatagtt
    15541 tcggcagaat gtactgtagc gaaagtttaa atgaatttaa caaggtagcc tccaggccat
    15601 tgttttgtcg ttcctgatcc ggcctacatg ccggatcctg aaaaaaaggg gacgattcta
    15661 acgacggtta gcttaattgt cagtcatccg tatcagaaaa gtttttacgc gtttttacct
    15721 gcgccatgct ttccagaata cggtgatagt tttcgaaacg ggtttccgcg attttccctt
    15781 cctcaaccgc ttcccggata gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt
    15841 tacacagacc taaatagtca tggaattcga caaagccctg agtgatttgt tccggctcca
    15901 ggtgccagag accaaactca cgcacacctg gggaatcaat cacatcaccg ccgtgcggga
    15961 agtgatacag tcgtgcggcg gtcgtggtgt gctgaccaag ccccgagttg tcagagacat
    16021 cgttggtcag gatctctttt tgcagaccca gcaacgcgtt cagcaggctg gatttgccaa
    16081 cgccagactg cccggcaaaa atgctgatgc gcccggtcaa cgcctcttcc agcggtttca
    16141 gcccatcctg agtatgactg gaaaccatca atacgcgata accgatattg cggtagatat
    16201 ccatctgctc gttgacgaac tccatgcctt cgtcgtccag caggtctatc ttgttgagca
    16261 caataatcgg ctcaatctgc aaggtttcgc aggccaccag gtaacggtcg ataatattga
    16321 gcgacagctc cggcaaaatg gcggagacaa tgacaatctg gtcgatgttg gcagcaatag
    16381 gtttcacacc gtcgtagaaa tccgggcgcg tcaacactga ggtgcgctca tgcaccgctt
    16441 ccacgatccc tttgacattt acgccttccg ccgccggttt acccggacgc cagactacgc
    16501 ggtcgccggt caccaacgaa cggatggtac ggcgaatatt gcagcggtga acgtcgccat
    16561 cggcggattc cacatcagcg tgcataccaa agcggctgat gacgatacct tcatcaggct
    16621 cgccaaacag attgtcgtcg tagtcgggct tctccttaga cgttttaaga cgacgctggt
    16681 gattggcgtt cacgcggcgc tgctggcctt tggagagttt atttttactc aatcgtacag
    16741 actcctggtc gcccctgatg ggcaaaacat ctatgataca cgcaattgtg gatcaatata
    16801 gtcactgtga atgggtggaa aatagcatga gtgccaatga aaacaacctg atttggatcg
    16861 atcttgagat gaccggtctg gatcccgagc gcgatcgcat tattgagatt gccacgctgg
    16921 tgaccgatgc caacctgaat attctggcag aagggccgac cattgcagta catcagtccg
    16981 atgaacagct ggcgctgatg gatgactgga acgtgcgcac ccataccgcc agcgggctgg
    17041 tagagcgtgt gaaagcgagc acgatgggcg atcgtgaagc tgaactggca acgctcgaat
    17101 tcttaaaaca gtgggtgcct gcgggaaaat cgccgatttg tggtaacagc atcggtcagg
    17161 accgtcgctt cctgtttaaa tacatgccgg agctggaagc ctacttccac taccgctatc
    17221 tcgatgttag caccctgaaa gagctggcgc gccgctggaa gccggaaatt cttgacggct
    17281 ttaccaagca ggggacgcat caggcgatgg atgatatccg tgaatcggtg gcggagttgg
    17341 cttactaccg cgagcatttt atcaagctgt aaaatttttc ctggtcacgt aagcgccgga
    17401 atccacttgc ggcggcgtga ttttgacgct aaattgaacg ccttgctgat aatttcagca
    17461 cttgagataa aaactcaaaa aaaatttttt aggggggttg cagagggaaa gatttctcgt
    17521 ataatgcgcc tcccgtaacg acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt
    17581 a
//
LOCUS       DACADF010000097        17450 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.099, whole genome shotgun sequence.
ACCESSION   DACADF010000097 DACADF010000000
VERSION     DACADF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.099"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..1078
                     /locus_tag="ISB30_003153"
     CDS             <1..1078
                     /locus_tag="ISB30_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAP1990758.1"
                     /translation="YLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLA
                     LFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTA
                     TWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYT
                     IWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLS
                     RPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVF
                     GLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTHNISLV
                     MTEADKALREAKSEGGNKVIIHHI"
     gene            1259..2749
                     /locus_tag="ISB30_003154"
     CDS             1259..2749
                     /locus_tag="ISB30_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAP1990759.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVPPEQRRAICEAFESANNTHGLNLTAHK
                     YQGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2792..3295
                     /gene="yeaK"
                     /locus_tag="ISB30_003155"
     CDS             2792..3295
                     /gene="yeaK"
                     /locus_tag="ISB30_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAP1990760.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3296..3400)
                     /locus_tag="ISB30_003156"
     CDS             complement(3296..3400)
                     /locus_tag="ISB30_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990761.1"
                     /translation="MNDQMFVETLIITSSFFAIAAVLVLSVLLIERTG"
     gene            3570..4016
                     /locus_tag="ISB30_003157"
     CDS             3570..4016
                     /locus_tag="ISB30_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP1990762.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3973..4794)
                     /locus_tag="ISB30_003158"
     CDS             complement(3973..4794)
                     /locus_tag="ISB30_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1990763.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPGSSHPKI
                     RTMVEMMAKEPVEWGSLRQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4891..6072
                     /gene="nimT"
                     /locus_tag="ISB30_003159"
     CDS             4891..6072
                     /gene="nimT"
                     /locus_tag="ISB30_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HAP1990764.1"
                     /translation="MTCSTSLSGKNRIFLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISQGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6127..6474
                     /locus_tag="ISB30_003160"
     CDS             6127..6474
                     /locus_tag="ISB30_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAP1990765.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWNKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6496..6750)
                     /gene="yoaF"
                     /locus_tag="ISB30_003161"
     CDS             complement(6496..6750)
                     /gene="yoaF"
                     /locus_tag="ISB30_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAP1990766.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6933..7958
                     /gene="dgcP"
                     /locus_tag="ISB30_003162"
     CDS             6933..7958
                     /gene="dgcP"
                     /locus_tag="ISB30_003162"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAP1990767.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            7991..8089
                     /gene="yoaK"
                     /locus_tag="ISB30_003163"
     CDS             7991..8089
                     /gene="yoaK"
                     /locus_tag="ISB30_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAP1990768.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8092..8166
                     /gene="yoaJ"
                     /locus_tag="ISB30_003164"
     CDS             8092..8166
                     /gene="yoaJ"
                     /locus_tag="ISB30_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAP1990769.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8225..8473)
                     /locus_tag="ISB30_003165"
     CDS             complement(8225..8473)
                     /locus_tag="ISB30_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP1990770.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8621..8803)
                     /locus_tag="ISB30_003166"
     CDS             complement(8621..8803)
                     /locus_tag="ISB30_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HAP1990771.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8807..9166)
                     /gene="yeaR"
                     /locus_tag="ISB30_003167"
     CDS             complement(8807..9166)
                     /gene="yeaR"
                     /locus_tag="ISB30_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HAP1990772.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9339..9977)
                     /gene="leuE"
                     /locus_tag="ISB30_003168"
     CDS             complement(9339..9977)
                     /gene="leuE"
                     /locus_tag="ISB30_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HAP1990773.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSETKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10104..10271)
                     /locus_tag="ISB30_003169"
                     /pseudo
     CDS             complement(10104..10271)
                     /locus_tag="ISB30_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10260..10430
                     /locus_tag="ISB30_003170"
     CDS             10260..10430
                     /locus_tag="ISB30_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP1990774.1"
                     /translation="MTTGGKQPGDIDFYVVRKNTEGEYSSPGGRVNEGTENEVVIQES
                     VFTRRGVERIWR"
     gene            complement(10520..11635)
                     /gene="rnd"
                     /locus_tag="ISB30_003171"
     CDS             complement(10520..11635)
                     /gene="rnd"
                     /locus_tag="ISB30_003171"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAP1990775.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(11717..13402)
                     /gene="fadD"
                     /locus_tag="ISB30_003172"
     CDS             complement(11717..13402)
                     /gene="fadD"
                     /locus_tag="ISB30_003172"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAP1990776.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(13607..14188)
                     /gene="yeaY"
                     /locus_tag="ISB30_003173"
     CDS             complement(13607..14188)
                     /gene="yeaY"
                     /locus_tag="ISB30_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAP1990777.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     EPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(14228..14923)
                     /gene="tsaB"
                     /locus_tag="ISB30_003174"
     CDS             complement(14228..14923)
                     /gene="tsaB"
                     /locus_tag="ISB30_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP1990778.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLHDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(14981..16891)
                     /locus_tag="ISB30_003175"
     CDS             complement(14981..16891)
                     /locus_tag="ISB30_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP1990779.1"
                     /translation="MTDDFAQDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTIDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKTGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            17023..17367
                     /locus_tag="ISB30_003176"
     CDS             17023..17367
                     /locus_tag="ISB30_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAP1990780.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4320 a         4428 c         4204 g         4498 t
ORIGIN      
        1 ctatttagtt gaaaccattg taattatcca taaaccaatc aacggcagta cactgatcca
       61 gacaaagtcg aatgatgttt ctattttcta tattttccgc caactcagtt tcatttgttt
      121 aacctcgctg gcgctctttt gttatgggaa agacaacatc cttgacaaca ataagaaaaa
      181 aactggcatt ctgttgctgg cgctgattcc ttttttagtt tttccccttc tggcacacaa
      241 tctgagcagt tataacgctg actattcttt gtatgtcgtc gattactgcc cggacaacca
      301 tactgcgacc tggggaatca actatacaaa aatattggtt tgtttgtggg catttttact
      361 gttctttatt atcatgcgta cacgattagc cagcgaacta tggccgttaa tagcattatt
      421 atgtctggca tcgctatgct gcaacttact tctactgact ctggatgagt ataattacac
      481 catctggtat atcagtcgcg ggattgaagt ttccagtaaa ctgtttgttg tgtcttttct
      541 gatttataac atttttcagg aactgcaact ctccagcaaa ctggcagttc atgatgtgct
      601 gaccaatatt tataatcggc gctacttttt caacagcgta gagtcattat tgtcgcgacc
      661 tgttgttaag gacttttgtg tcatgctggt tgatattaat cagttcaaac gcatcaatgc
      721 ccaatgggga catcgtgtgg gtgataaagt gctggtatca attgtcgata ttatccagca
      781 aagcatccgc cccgatgata ttttagcgcg actggagggt gaggtgtttg gcttgctatt
      841 taccgaactc aatagtgccc aggcaaaaat cattgcggaa cgtatgcgta aaaatgtcga
      901 actcctgacc ggctttagta acagatatga tgttcctgaa caaatgacca tcagtattgg
      961 cacggttttt tcaacgggtg acacgcataa tatctcgctt gtcatgacgg aagcagataa
     1021 agccttacgc gaagcgaaaa gcgagggggg caacaaagtg attattcatc atatttaagt
     1081 gcaaaaatat tcacagccat gctttttgcg tggcttttgc atacaattta ttactattac
     1141 ccctaaattt cccctcccca gcggcgcaga gatgagtata attagcgccc ctgtgccagg
     1201 ccgcaatcga actttatctg gttttctcgt ttcactaacc gaaggagtgc catttatcat
     1261 gaaattgcac catagaatgc tccggcattt tatcgccgca agtgtcattg tgctgacatc
     1321 ttccttcctt atttttgaac ttgtcgccag cgacagagca atgagtgcct atctgcgcta
     1381 tatcgtgcag agagcagact cttcctttct ttatgataag tatcagaatc agagtattgc
     1441 cgcgcatgtg atgcgcgctc tcgctgctga gcagtcggaa gtaccgccag aacaacggcg
     1501 cgctatctgc gaggcttttg agtctgccaa taatacccat ggcttaaacc tgactgctca
     1561 taaataccag ggcttacgcg gcacactaca aaccgcatcc actgactgcg acacaattgt
     1621 ggaagctgca gcactattac ccgcttttga tcaggcagtg gaaggcaacc gccaccagga
     1681 tgattacggt tcaggtcttg ggatggccga agagaaattt cactattatc tcgatctcaa
     1741 tgaccgctat gtctattttt atgagccggt taatgttgaa tactttgcga tgaataactg
     1801 gtccttcctg caatcaggaa gtattggtat cgatcgcaaa gatattgaaa aagtatttac
     1861 cgggcgtacc gtattatcga gcatttacca ggatcagcgt actaaacaga acgtgatgag
     1921 tttgctgacg ccggtatatg tcgcagggca gctaaaaggg attgtgctgc tggatattaa
     1981 caaaaacaat ctgcggaata tcttttacac tcatgaccgc cctctcctct ggcgttttct
     2041 caatgtcacg ctaaccgata cagattcggg gcgcgacatt atcatcaacc agagcgaaga
     2101 taatctgttc cagtatgtca gttacgtcca tgacttaccg ggcggcattc gtgtctcgtt
     2161 atccattgat attctttact ttatcacgtc ttcgtggaaa agcgttctgt tctggatttt
     2221 gacggcgtta attttgctga atatggtgcg gatgcacttc cgcttatacc aaaatgtgtc
     2281 gcgagaaaat attagtgatg cgatgactgg actctataat cgcaaaattt taacccctga
     2341 actggagcag cggttgcaga aactggtgca atccggttct tcggtgatgt ttattgctat
     2401 tgacatggac aagttaaagc aaataaatga caccctcggt catcaggagg gggatttagc
     2461 gattacgtta ttagctcagg cgattaaaca gtcgattcgt aaaagtgatt atgccatccg
     2521 actcggtggc gatgaattct gcatcattct tgtcgattcg acgccgcaaa ttgcagcaca
     2581 actgcctgaa cgtatcgaaa aacgtctgca acatatcgcg ccgcagaaag agatcggctt
     2641 ctcttccggt atttacgcga tgaaagaaaa cgatacgtta catgatgcgt ataaagcttc
     2701 cgatgagcgt ttatatgtca ataagcagaa caaaaacagc cgttcatgat aaccttctgt
     2761 ggttgtttgc ttgtaatctc aggagcatga aatgactgaa atggctaaag gaagcgtgac
     2821 gcatcagcgg ttaatcgctt tattatcaca agaaggtgct aacttccgcg tcgttaccca
     2881 tgaagcggta ggtaaatgtg aagcagtgtc agaaattcgt ggcaccgctt taggccaggg
     2941 ggctaaggca ctggtatgta aagtcaaagg caatggcgta aatcaacatg ttctggcgat
     3001 cctcgctgcc gatcaacagg ccgatctgag ccaactcgcc agccatatag gcggattacg
     3061 cgcttcgttg gccagcccgg cggaagtcga tgaactcaca ggctgtgtct tcggcgcgat
     3121 cccccctttc agcttccatc caaaactcaa actggttgcc gaccccctac tcttcgaacg
     3181 atttgatgaa atcgccttca atgcaggcat gctggataaa tccgttattt tgaaaaccgc
     3241 ggattatttg cgtattgcgc aaccagaact ggtcaatttc cgccgcactg cgtaactagc
     3301 ctgtccgttc aataagcaga acggacaaaa ccagcacagc agcaatggca aaaaacgatg
     3361 acgtgataat cagtgtctcg acgaacattt gatcgttcat agtatgcccc cgggagatag
     3421 ctgttttcac gttattgtta acgtacacaa atggcagttt gatgacagtt cgtctgattt
     3481 tttatgcaaa aaacgtaaag attttctact tcctgcctgc agcaagcgta aagtaagcag
     3541 gcttattatt ttttggcaag gaaaccacga tgtttgatgt cactttgctg atcctgctcg
     3601 ggttagctgc gctgggcttt atcagtcata acaccactgt cgccgtttca attctggtgt
     3661 tgattattgt ccgcgtcaca ccgttaagca cctttttccc gtggattgaa aaacaggggc
     3721 taagtatcgg gataatcatt ctgacgattg gcgttatggc ccccatcgcc agcgggacgc
     3781 taccgccctc aaccctgatt cactcgtttc tgaactggaa atcgctggtg gcaattgcag
     3841 tcggcgttat tgtctcctgg ctgggggggc gcggcgtgac gttaatgggc agccagccgc
     3901 aactggtcgc cgggctgctg gtgggcactg ttttaggcgt ggcgctgttt cgcggcgtac
     3961 cggttggccc gcttattgcc gcgggtctgg tttcgctgat tgtggggaaa cagtagttaa
     4021 tcccgcaata tatcggcctg gcgtttgccc caggcctttc ttaaacatag taataaaggc
     4081 cgttgttgaa tcatacccca gtgtatgagc aactttctgt acagtatcgc ctttcactaa
     4141 tccctgaagc gccataatca gctgtaactg ctgacgccac tggcgaaaac tcaaccccgt
     4201 ctctttcact atcagccggg caagattgcg ttcactcatc gcaaaaaagc cagcccattg
     4261 cctcaatgag ccccactcga cgggctcttt cgccatcatc tccaccatcg tgcggatttt
     4321 aggatgagaa gaaccgggca agtgcagctg ttgttgtggt tgctgaggga gttcatcaaa
     4381 caggacctga attaatctgc gggtcatcgg ctcagcacgc tgagtcgtcg tcctgtttgc
     4441 cagggttaaa attaattccc ggcataaggg cgatattttc aatgtgcaac atgttgtcgg
     4501 cattgtcact gcgctgggct cgataaacag aaaacagagt tcggcgttag ctgtcacttg
     4561 attactatgt ttcaccccgc ccggtatcca aaccgcatat tgtggcggca ccatccacaa
     4621 agcattttct accgtacagg taattgcacc atgtagtgcc agtatcaact gccctttgcg
     4681 gtgctgatgt acaggactgg ttaactcatc cgttccggcg tgaatacaaa atgcgaccgc
     4741 ggcttcatgg tgtcggtcgg gttcatagcc attgagattc aacctgtgca tcattttgtc
     4801 cgaatttagc gatattttgt cattttagct tgattcaaca taacaataaa aacggtaagg
     4861 tacagcctcg tttgtaacaa tgagaagcat atgacctgtt caacttcatt aagcggtaaa
     4921 aacaggattt tccttatcgc tggcattctg atgattgcca caacattacg cgtcaccttt
     4981 accggcgcag caccgttact ggatacgatt cgttccgctt actcgctaac gacggcgcaa
     5041 accggcttat tgaccaccct gccattatta gcctttgcgc tgatctcacc gctggccgcc
     5101 ccggtggcgc gacgttttgg tatggaacgt agcctgtttg ccgcgttact tttgatctgt
     5161 gcaggtatcg caattcgctc tctcccttcg ccttacttat tatttggcgg tacagcggtc
     5221 attggcggtg ggattgcatt aggcaatgtc ttactgccag gattaattaa acgcgatttc
     5281 cctcattccg tcgccagact taccggcgca tattccctga caatgggagc tgcggcggca
     5341 ctgggatcgg cgatggtagt gccgctggct ttgaacggtt ttggctggca aggcgcgttg
     5401 ctcatgctga tgtgttttcc tctgctggct ctttttttat ggctgccaca gtggcgaagt
     5461 caacaacatg caaatttgag tacctcacgc gccttacata ctcggggtat ctggcgttcg
     5521 ccgcttgcct ggcaggtaac attgtttctt gggatcaact cactggtcta ttacgtgatt
     5581 attggctggc ttccggcgat cctcatcagt caaggctata gcgaagcaca ggcgggttca
     5641 ctgcatggtt tgctgcaact agccacagca gctcccggtt tgctgatccc acttttctta
     5701 catcatgtga aagatcagcg tggtattgca gcgttcgttg ccttgatgtg cgcagtgggc
     5761 gcggttgggc tctgctttat gccagcacac gcgatcacct ggactctgct tttcggtttt
     5821 ggttccggcg caacaatgat actggggttg acgttcattg gtcttcgggc aagttctgcg
     5881 catcaggcgg cggcactctc ggggatggca caatccgtcg ggtatttgct ggcagcctgt
     5941 gggccgccgc tgatgggtaa aatacacgat gctaacggtg actggtccgt accactgatg
     6001 ggtgttgcaa tactttcact actgatggcg attttcggac tttgcgccgg gagagacaaa
     6061 gaaattcgct aatatccggt gctatagtga cgtgacaaat catgcgtgaa agggagaaca
     6121 aatacgatga atattcagtg caaacgcgtt tatgatccgg ctgaacagag cgatggttat
     6181 cgcgtactgg tcgaccgcct ctggccacgc ggtatcaaaa aaaccgattt agcccttgat
     6241 gagtggaata aagaaatcac gccgtcaacg gagctgcgca aagcctttca cggcgaagtc
     6301 gtcgattttg caacctttcg cgagcaatat cttgcagaac tggcgcaaca cgagcaagaa
     6361 ggaaagcggc tggcagacat cgccaaaaaa cagccgctga ccctgctcta ctcagcaaaa
     6421 aacaccacgc agaaccatgc gctggtgctg gcagactggc tacgtagctt gtgattttag
     6481 tacagcatcc ggcggttatt tttcaccagc cggatggtcc cgtcgccaca atgcccattc
     6541 atcaatcgtt tcaccgcccg gtaatttgca attgttgctg accccttgcg ctgtctgcac
     6601 aggaatgagc gtcccgccct tctgctggca atagaccgac gccggatttg ccataccaat
     6661 ctgcggcggt ttaggtgctt ctggctgaga aggggttgaa caaccagcca ggaccagcaa
     6721 gcaaggcaga acaaaactga taattttcat ttattgatct cacatattta tccaggctta
     6781 gagtatcgcg gtatcgtttt gttttgcagc actattttta ttacattcac tcaaaacata
     6841 ttacgccttg tttcatcttt gttgatgatg ttttatcatg cctgcaaaga ttaaataatc
     6901 agcatttacc cgccgtatcc tggagttgtt tcgtgtcaga tcagattatc gcccgcgtct
     6961 cgcaatccct tgccaaagaa cagtcactgg aaagtctggt tcgacagctt ctggagatgc
     7021 tggaaatggt cactgatatg gagtcaacct acctgaccaa agtggatgtc gaagcgcgcc
     7081 tgcagcatat aatgtttgcc cgtaatagcc agaaaatgca catcccggag aattttaccg
     7141 tctcgtggga ttactcgtta tgcaaacgcg ccattgatga aaactgtttt ttcagcgatg
     7201 aagtccccga ccgttggggc gactgtattg cggcacgcaa tcttggcatc accacatttc
     7261 tgagcacgcc aattcactta ccggatggat cattctatgg cacgctttgc gccgccagca
     7321 gtgagaagcg ccagtggagt gaacgcgcgg aacaggtctt acagttattc gccggactga
     7381 ttgcacaata tattcaaaaa gaggcactgg ttgaacagct gcgcgaagct aatgctgcgc
     7441 tgattgcgca atcgtatacc gactcgttaa ccgggctacc gaatcggcgg gcgatttttg
     7501 aaaatctgac gacgctgttt tctctcgccc ggcatcttaa ccataagata atgatcgcgt
     7561 ttatcgatct ggataacttc aaattaatca atgatcgttt tggtcataat agtggcgatc
     7621 tgtttctcat tcaggttggc gagcgcctta atacgctcca gcaaaatggc gaagttattg
     7681 gtcgtctcgg cggtgatgag tttttggttg tttcactgaa caacgagaat gcggatattt
     7741 cgtcgctgcg agaacgtatt caacaacaaa tacgcggaga atatcactta ggtgatgttg
     7801 atttgtatta tcctggtgcc agtcttggca tagtagaagt cgatcctgaa acgaccgatg
     7861 cagacagtgc cctgcatgct gccgatatcg cgatgtatca ggagaaaaaa cataaacaga
     7921 aaacaccttt tgtcacgcat tcagcgctac attcctgagg cgtattcaca tccttttgat
     7981 tggtgataac atgcgaatcg gtattatttt tccggttgta atctttatta cagcggtcgt
     8041 atttttagca tggtttttta ttggcggcta tgctgccccg ggagcataaa gatgaaaaaa
     8101 acaacgatta ttatgatggg tgtggcgatt attgtcgtac tcggcactga gctgggatgg
     8161 tggtaacgtc acatctaaaa aatagcaaag gctgcctgtg tgcagccttt gtgcaattta
     8221 cgcgttaact tttaatcttc ctgtagataa atagcacaac aatcgcacca ataacggcaa
     8281 ccacgaagct gccaaaattg aagccatcga ctttaccaaa gccaaacagc gtgctgatcc
     8341 atccgccgac tacggcaccg actatcccca gcaggatagt cataaagaat ccaccaccat
     8401 ctttacctgg catgatccac ttcgccagaa taccggcaat aagcccaaaa ataatccatg
     8461 acagaatgcc cattgtttcc tcacttatct gttttgcatt agcgggttag tcgctgataa
     8521 aaagcatagc acaacatcgg gaggggaaga tttgtgacga gcatcacgga ggaatttttt
     8581 gcgatggcgc agaaattgcg ccatcaacga tcagtgataa ttaccaacca caaacatcat
     8641 gttcgttttc cgtgtcataa gaacgcacgg tattcaccag atcttttatc acttcagccg
     8701 ccacttctgg caccagcaaa gtcatcggcg tctctgtttc ataatcgaca gaaacgccat
     8761 tgctgttatt ggtgacggtc acggtatacg ttgctttgcc catgattcat ttcccgttat
     8821 gaatgacttt ccgttgttgc gcgccttcca tcaggacttc aggagcaacg aagaagtcaa
     8881 tgttgaaata agtatcgtca gtcatggctt caatgttgtg ccacttttct ggagggaaca
     8941 ccgcaaactg ccccgcttcg ataaggatca cctgatcagg ctctgcactg tgttcatcag
     9001 cgtagccgag atatttgacc gccccatgca taacggaaag gcgtgggtaa acccccgggc
     9061 gcgttccttt atcaagatga cgttcgaata ttccggcagg cgcagtttgt ttattccaga
     9121 aaggcgttga gcgcgtatga atataattct gtgggatttg cagcatcctt ttccctcctt
     9181 cggtgaatgc gctggaaacg gtttattcca gccgtttcag ggtacgcctg ataatttgca
     9241 ttttaaatac catttattgg ttacttttta gcaccgtatc agagaagaat cagggaggat
     9301 tacaggtggg aagcccgcgg caacgcgggc tgaaagcatc aggattgcag cgtcgccagt
     9361 cgggcggcga aacccacgaa catcaaacca atcagtgagt tgcccacttt agccagtttc
     9421 tttttggtac gtatgtactg cgtgacaaaa gccccagaaa taatcaggaa gctcaaatag
     9481 cagaaactca ccagttccag cgtcgtcgcc agaataaaga atgaaattcc cgtatgtggg
     9541 gcattaacat cgataaactg tacgaaaaac gacacataga acaaaatggc tttcggatta
     9601 gtcaggctca aaattaacgc gcgtttaaaa atggcaccgt attggggctc atcggatttg
     9661 gtctcgctat ttttaccttt cagggtcgcg tagagaattt tactccccag atagagcaaa
     9721 taaaacgcac caagataacg tacgatgttg aataatatcg gggtggtctt aattaatgtc
     9781 gccactccag cccatgccag aaacatcaat accgcatcgc caataaatac accacaggcc
     9841 gcaagataac cgcctttcat accgctactg acgctatttt tgagtacaaa cagggtattt
     9901 ggccctggca ccaacacaat aaaaatggcc ccaaccagat aggtccagta attcagaacc
     9961 ccgtattcag cgaacacatt aacctcttta attatctttc gatcatgcgc gattaaaggt
    10021 gaatatgcta accaatctgt agcggcttag aaaggagaaa atcaggtttt aactcgatat
    10081 caacccgata attgaatcat taatcaggca tgctccagtg aaaaattcgg gtagtgctcg
    10141 gcaaaatact ggcgtaaaaa ttctaccgtt atccgcactt tcgctgacgt cgacagcttt
    10201 gaaacataaa cggcccagac attcgctggc tggtaatatt ccggtagcac ttgtactaaa
    10261 tgaccactgg cgggaaacag cctggcgaca tcgattttta cgtggtccgg aaaaacaccg
    10321 aaggcgaata ttcctcgcca ggcggtagag tgaatgaagg aacagagaat gaagtcgtca
    10381 ttcaggaatc ggtatttact cgtcgtggtg tcgagcgcat ttggcgttaa gctcaaataa
    10441 gttcatttga accgcctgct gttggatttg tagtttgccg aagccggact gagcgcgccc
    10501 agtccggctt cggaagattt tactgtggat attcctgcaa taaattgtgt aatgcttccg
    10561 ccatcagctc accacgccag ccggaaatca gctccggcaa attgttctgc ggtttcagtt
    10621 tccagtgcca gttcagcagt tggttgattt gccgacgcga tgccagcaat tcggcgctga
    10681 tcttgtgcgt ttcgctgacg tcagtaatca gcgacttaat cgctttaaac gctttacgat
    10741 aacccggcat gtccatcagg ttaagcatcg gctgcggtaa ggcctcttcc ggcaatgcct
    10801 gcgctttttc caccagcgcc agcaacgttt taccgtggaa gcggatttcg ctaccggata
    10861 agcccaggct gtccagttcg cccagacttc ccggcatata acgcgctacc gaccacaaat
    10921 gctcttcacg cacgacaaag ttcaccgcca gatcgcgctc tcgcgccttg cgcaatcgcc
    10981 agtcggctaa cagttgcaga caggccagtt ggcgtgtgcg taactgccag gcattggtga
    11041 tatcacgcca ggcgtcttcc ggcgcaacga cttcctgacg acgcatttgc atcaggcggc
    11101 attcatccag cgccgcaggt agccagccgg aggcctccgt ttctaccata agctttgcgg
    11161 tgatcggtaa cagataccag acatccgccg ctgcgtattc acactgacgt tcggtcagcg
    11221 gtctggccag ccagtcggtg cgcgattcac tcttgtccag cgtaacgccg gaatactctt
    11281 ccaccatgga agcgaaaccc catgacatcg ggcgtccgca gaaggcagca aggatttgcg
    11341 tgtcaatcaa gggttgtggt aattcgccaa agacattgag gaacacttcc agatcttcac
    11401 tgcctgcatg gagaaatttt gtgatggacg gatcgcgcag gatcgctttc agcggtgacc
    11461 agtcggtgat cccgagtgga tcgattagcg ccagatgctc accatcaaaa agttgaatta
    11521 aacccagctg cgggtaataa gtgcgcgtac gaacaaattc agtatccagg gctatcgccg
    11581 gaaaggcacg gacggcttca cacaaagaag ccagcgcatc gtccgtggta atcatttggt
    11641 aattcaaatt gtattctctt tagtgggcgt caaaaaaaac gccggattaa ccggcgtctg
    11701 acgactgact taacgctcag gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt
    11761 ctcgtcgcaa aatttttccg acgttagatt tcggtaactc atcacgaaac tccaccagct
    11821 tcggtacttt gtagcccgtg agctgacggc ggcaaaaggt caccagtgac tcttcggtaa
    11881 gcgatggatc ttttttcact acgaagattt tcaccgcttc accactggag ccggaaggta
    11941 cgccaacagc cgcgacttcc tgtacgccag gatgctgcat gacgacatct tcaatctcgt
    12001 tgggatagac gttaaaaccg gaaaccagaa tcatatcttt tttacgatcg acaatgcgca
    12061 ggaatccttc ttcatccatc accgcgatgt cgccggtgtg taaccagcca tttttgatga
    12121 tttcatcggt agcatccgga cgctgccagt aacccagcat cacctgcggt cctttgacac
    12181 aaagctcacc tggttgacca ggtggtactt cattatcatc atcatccacc agtttggctt
    12241 ccgtcgacgg caccggcaaa ccgatgctac cactatgata atcaatatca tacgggttaa
    12301 cgctgaccag cggcgcgcac tcggtcaggc cataaccttc cagcagatac tgtccagtca
    12361 gtttaaccca acgttctgca accacttgct gaacaggcat cccaccgcct gcggaaagat
    12421 gcagactgga gaaatccagc tgctggaact ctttattgtt cagcaacgca ttgaacaagg
    12481 tgttaacgcc agtgatagcg gtaaacggat atttcgctaa ctcttttacc agccctggaa
    12541 tatcgcgcgg gttagtgata agcaggttct gccctcccag ttcgataaac agcaggcagt
    12601 taatggtcag tgcaaaaatg tgatacagcg gcagcgccgt caccaccagc tctttgcccg
    12661 gatgcaacag cggaccatag gtcgcgttaa cctgttccag gttcgccagc atattgcggt
    12721 gagtcagcat cgcgcctttc gccacaccag tggtgccgcc ggtgtattgc aggaaagcta
    12781 aatcttccgg caccaattct ggtttgacgt actgcatccg gtagccgttg tgcagggcgc
    12841 tacgaaatga aatggcatct ggcagatggt atttcggcac cagacgcttg atgtatttaa
    12901 caacgaaatt gactaccgtg ccttttgccg tagatagctg atcgcccata cgggtcagaa
    12961 taacgtgctg aacggcggtt ttatcaacca ctttttccag agtgtgggca aagttagaca
    13021 cgataacaat cgccgatgcg ccgctatcgt taagctgatg ctcaagctca cgcggggtat
    13081 acaacgggtt aacgtttacg acgatcatcc cggcacgcaa aatgccaaac agcgccaccg
    13141 gatattgcaa taagttaggc atcatcaacg caacgcgatc gcctttcttc agccccaacc
    13201 cttgttgcaa ataagcggca aacgcgcgac tgcgttcttc cagtttgcgg aaggtcatca
    13261 cctcccccat attcacaaac gcgggttgat cggcatagcg tgcgaccgac tgctcaaaca
    13321 tatctaccag agattgataa cggtcagggt tgatctccgt cggaacgtcc gcgggataac
    13381 ggttaagcca aaccttcttc aattcttcac ctctaaaatg cgtgttcgtc gtcatcgcaa
    13441 ccccaagtga taaacatgct attaacataa tattaactca tcttaccagc ttgataatta
    13501 cccaaggaaa aggttgcgaa gcgcgtcact atttattttt atctttaccg taagaatgca
    13561 gaaacagcgg accggccgct gtttcttttt ctttaaaaac aagcaattat tcagttacaa
    13621 ctgtttgtac tctcgcgggg ccgggattat accagcccca tccgccgtag ccatagggcc
    13681 agccacgacc gccataaaac catggttcaa tcggctgagg cggcataatc acctgctggg
    13741 ttaaatgcca acgtttgtaa cccgttactt gcatcaccat aaatttatag ggcgtattac
    13801 cgattttgcc gtcaaccgca ccggtgattg gcccgactac cgtcaccagt tgtccgcgga
    13861 aatccaccgg gtccagaaaa ccgttcacat cggcataaat gcgaccgcga gaaggttctc
    13921 ccagcgtcgg tctggctccg ctgtccagtg gtacggtggc aatttccagg cgggttttcc
    13981 cttgctggtt ttgtacagca accactttgc cgccaaagcg agcctcctga ccaacgtaca
    14041 gctgcggcgc actcatcacc cgaactaaat cttgttgcgg cgtgggacta ctgcctttaa
    14101 tggcgtccgg cacagtaaca caaccgctca gcattagcgc aaacgtacct gccagtatgc
    14161 ctttgataac attcttttga accgccatga tgcgactcct ttttttctca gggcatactc
    14221 ttaagattca ttctttgccc ggaagtttct tccatgcgac attgttacgt aaataaactg
    14281 gttcggcatg ttccaccgcc accgttttac cctcggcaaa catctggcac gcaatcggca
    14341 gcatatcttc tgcagcgggc agtaacactt cgccatcgtg caaaaccagc ccgctctctt
    14401 taccgagatc cggccaggct tgccagcccg tccctaccgt cacccattcg ccggaaagct
    14461 gttgcattcg ttcatgaacc agttcgggtt tgagtacggc ttcggtttct tcaccgtgcc
    14521 agataccgtt ttcatcacgc tgatattcgg cccagtaaac ttcgcccatt cgcgcgtcaa
    14581 tagctgccag cacgcgggtt gcgccgtttt tgcgccacgc cccttgcgcc atcgtcatta
    14641 gcgtggagac gccaatcatc ggcagctctg cgccaagtgc cagcccttgc gcaataccaa
    14701 taccaatgcg cacgccggta aagctaccgg ggccgcgccc gtaagccaga gcgtttatgt
    14761 cagtcaggga agttccgctg gtggtcagga tatcctgcac catcggtaag attcgttgag
    14821 tatgttcacg agggcaaagc tcaaaatgag cgttgacagt accgtcgttc cacagggcga
    14881 cagagcaggc ctctgtcgcg gtatcgatag ccagaattcg catgggtctt cgtgcttaga
    14941 tcaataaaaa ggcgcgcatc ataccatact ccgtaacaaa ttacctggag gatggtatcg
    15001 caaggaaacg aaccgcacgg gcaatgtcac gggtgcgcgg cgctggcggc agactggcga
    15061 gaaacgtcgc gccgtaaggg cgcatcacca gccgattgtc gcaaatcacc agcacgccgc
    15121 gatcgtcggc gtcgcgaatc aatcgcccta ccccctgctt gagagtaatg accgcatccg
    15181 gcagttgtac ttcatcgaac ggatcaccac cgcgcaaacg gcaatcttcc atgcgtgctt
    15241 ttaatagtgg atcatccggc gaggtaaacg gcaatttgtc gataatcacc aatgacaatg
    15301 tatcgccacg cacgtccacc ccttcccaga aactgctggt tgccacaaga agcgcgttac
    15361 cggcgctgac aaattgctgc aacagttgcc ctttgctggt ttccccctgc aacaaaacgg
    15421 gaagcgtcat ggtagcgcgg aactgctcgg ccagatcgcg catcatggcg tgcgaggtac
    15481 aaagcataaa acaacgaccg ttgttagctt cgatgatcgg tcgcagcatt gccgccagtt
    15541 gccgtgcgga acccggttga ttggtttgcg gcaggttgcg cggcacacag agtaacgcct
    15601 ggcggctgta atcaaagggg ctgggtaaca gcaatgactc tgcctgttca atgccaagcc
    15661 gcgaggtgaa atgatgcaga tcgtcgttca ccgacagcgt tgctgaggta aagatccagc
    15721 taccggtttt ttgcgccatt aactctttga atttatccgc cacgctgagc ggcgtgagag
    15781 ccagagtaaa atggcgcgaa gtgcattcgt accagtagct gtagcccggc tgattgatct
    15841 cttttagccg tttcagccgt gtgcgatata acgtggcgcg ctcaaatgcc gcatccagca
    15901 aggcggaacg ccccagcgac agtttcgcca cgtcataaca aagttccaga gtgtcatcga
    15961 gcagtaaaaa tgcccgctga atttgcggat tagctaacag ctcacgcagg ttaccgcgat
    16021 aaccaggctc accgagttgc aggcgaaaat cctgcgcact ctgggcaaga cgatccgcgc
    16081 acttttgtaa ctgctgggtg tcttttaatt cggtgcggta ggcgatggtg atgtcttttg
    16141 ccaggtcgag cagttgtcga ctggagagtg actgaccaaa atactggctg gcaatgtcgg
    16201 gcagctggtg ggcttcgtcg aagatcatga cgtccgcctc cgggatcagt tcgccaaaac
    16261 cgctctcttt caccaccata tccgccagaa agagatgatg gtttaccacc accacatcgg
    16321 cgtccatcgc ttttttgcgt gctttgacca caaagcaatc tttatacatc gggcagtcgc
    16381 tgccgaggca gttatcgttg gtgctggtga ccagcggcca cgcctgtgaa tcttccgcca
    16441 cgctgacgca ggtgctgata tcaccatcga ttgtttgatt agaccaggag cgcagcagga
    16501 tcacatcgct taagatttgt accggcagat cgccccccgc caaagcctgc tgttcgagac
    16561 gttcgaggca gaggtagttt gagcgccctt tcagcagcgc cacattgccc gtgtatttca
    16621 atgcctttga gaccgttggc aaatcgcggc tgtagagctg atcctgcaac gcttttgagc
    16681 cggtcgagat tatgactttc tttttcgccc gcagcgcagg ggccaggtaa gcgtaggttt
    16741 tgcccgtacc ggttccggct tccaccacca gcggctggcc tttttctatc gcctgggtga
    16801 cggctaccgc catctgtcgc tgtggttctc gcggcttaaa gcctggtatc gctttcgcca
    16861 gctgaccgtc ttgtgcaaaa tcgtccgtca cactaccccc tgttgatttg aacagggatt
    16921 atgtcaggat gagggcgctt tcgccagttg aagtggtgac ggcgacctca cattgtggca
    16981 gtctttgcag cacgaaaatg gaagtataat gaggacaaaa tgatgactat cgttcgtatc
    17041 gatgccgaag cccgctggtc tgatgtggta atccacaaca acacgctcta ctacactggt
    17101 gtaccggaaa acctcgacgc cgatgccttt gagcaaaccg ccaacacgct ggcgcagatt
    17161 gacgccgtgc tggaaaaaca gggcagcaat aaatcgagca ttctggatgc caccattttc
    17221 ctggccgata aaaacgactt cgcggcgatg aataaagcgt gggatgcctg ggttgtcgcg
    17281 ggtcatgcgc cggtgcgctg cacggtacaa gcaggtttga tgaacccgaa gtataaagtt
    17341 gaaattaaga ttgtggctgc ggtgtaagct ttatcgaagc aaaataagtc agacgataat
    17401 ttatcgacaa tactggtcgg ttttgcaaaa atcgaccaga gattaagata
//
LOCUS       DACADF010000098        17326 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.100, whole genome shotgun sequence.
ACCESSION   DACADF010000098 DACADF010000000
VERSION     DACADF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.100"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            53..571
                     /locus_tag="ISB30_003177"
     CDS             53..571
                     /locus_tag="ISB30_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAP1990781.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            587..1126
                     /locus_tag="ISB30_003178"
     CDS             587..1126
                     /locus_tag="ISB30_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990782.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1219..2796)
                     /gene="guaA"
                     /locus_tag="ISB30_003179"
     CDS             complement(1219..2796)
                     /gene="guaA"
                     /locus_tag="ISB30_003179"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP1990783.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2865..4331)
                     /gene="guaB"
                     /locus_tag="ISB30_003180"
     CDS             complement(2865..4331)
                     /gene="guaB"
                     /locus_tag="ISB30_003180"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP1990784.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4493..5860
                     /gene="xseA"
                     /locus_tag="ISB30_003181"
     CDS             4493..5860
                     /gene="xseA"
                     /locus_tag="ISB30_003181"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP1990785.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFHPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKSLPSPAHCVGVITSKTGATLHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANKRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTRQLVHQNPQSRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGDTLTTRLGDGVVVSEVSAVTKTRK
                     PRKKT"
     gene            complement(5914..13995)
                     /locus_tag="ISB30_003182"
     CDS             complement(5914..13995)
                     /locus_tag="ISB30_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RatA-like protein"
                     /protein_id="HAP1990786.1"
                     /translation="MSVCLKPGKIIVLLGMLAAFMLSDFARAEVEWQTYPGSTGEFNG
                     TVPIADSASVPVYQGSVQLDPAASHDVAFSAKPNEFSVDDDAANLIVANPQDSEGDQF
                     STPPALRWENQTPPTVSLVWADAATPDTPLNPQPIANRSFCAQGLAGRSLVAWPQIDA
                     QQTIPLLYLLTSTGYPYEGTVVLADQKVTLNIAPAQGDLISVSASGYNETLGAAKTTV
                     GGTITLTVTTKDCQGNVAGNIPFIIKRKDAQNRQGAVNNTAPVVLDSTELTTTVTEYR
                     GTSDANGTATITVTQPNGPGVKTPLVVGISGIAQTSEAAVIFTVLTSPDVAQATMWGH
                     MAETVEAHGYTFSRPKLAAEVSNENATVVDHNETWSTFTWSGADSHCTVLPGMRHFGA
                     LATVIPSTVQTVLGWPMQGDYYWSSLAGLTGQHHAADVSNRGETQKPDSTTFLVSCVD
                     KPAPDVEPKIVLTPENYDDTAQAMKAKVGEDATMRLAITDTKNNDQPLAYYYFSLHLD
                     DGVNRKNQTDTAWEAHPVQIAGGSNFRQVDAHTYEGMTDANGQASLTLSQPGGAGVKT
                     HITARMRSDFNATDAKDVIFTVITSPDSDKARMWGHMRGIIESGSLYKRPLLADETEH
                     ELGTVRENNEDWALYDQNTSMQAECGVGHIPRQSSLESLFSAHPGNAIGTEYGWPTAQ
                     QGYLSAVEQATHSSVDLGNGSVDSYSGFKPNYLSCSGNEMVANVEVSTDHDVSVGTQA
                     QAKVGDTIVMTVRTINSLNNIPVPFTAFTITKGMGYNRAGQVSGFDDPSSGAITMDNT
                     QYGTSQSSKVYAGITDARGVATVEIKQPQGVGLKTVLSVTPVNSYLPNTVNYSVIFTT
                     PTSPDVTGAQMWGHMDETITVDSSTFTRPKLASEVASPDGTLTENNEIWARVSQANTS
                     STSKGGCDTNMLPRRSQLSALYNANSGNAVQTAHGWPTQRQPYWSNSPADKTPHFFTI
                     ALNDGAQTIGGDTPVYVSCLKTANKPASSITLEVVDKAQWNTGNSAATLKKGETLQVK
                     VTVKDAQGNALADMPFTLNRGDGYTRSGEKHVAGSGDALVAPVVVNGGLADETTLNDT
                     ATVYTAMTDNDGSKILNITRPDTHGTKTALTAMLYSDATKKSSIDTIFTVVTSPDSRL
                     AKMWGHMPETVTAADGTVFKRPLLLKELAYQTGRTSTSEDNENWALFNINYASFSTTY
                     SGCGTNYIPTQAGLTSLFANNAGNTMKTVQGWPVATRYLSNTSDNGSMEQRNYKAVDL
                     SNGTSAAVSSTTLELLTCQTAPIAAVSQILLEAADPVTLDTTYNVVKAKKSEESVVRV
                     ITKDAQGNLVGNTAFILTRANSVSRANSSATMSVGALTVTDAWGNTRNNFQSTSETIY
                     GVTGADGSTTFTLKQDNSTGLRTDLTAKLDTSSSVKAMLPVVFTVVTSPDSPKANFWG
                     HMAETVTASDGSVYKRPLLFGELTNNYARYSTFENGESWARFTSTQSADTSMNGCGTD
                     YMPTLDGLKSLYDANRGNAMNTVQGWPVNMSYLTNTPSNTQTGSRYYNVVQLNSGAVS
                     QIVSTVLALQTCRTTPLMTASQITLEASDPGQFVSIDSTLSAVKAKKGDEVSIRISTK
                     DAQGNLVGNTPFALKHANSINRQNVTSSQKVSVTTAAGATVDTSATTILYGVTGPDGT
                     TTMTLRQDASTGLRTDFYALLNDTGVSSDTLSVIFTVTTSPDTPLATNWGHMAETFTS
                     SEGVTFKRPFLKAELSGGTAKTANNEVWSLLTATEKADVSKAGCDEAYQPLTTDMQGL
                     YVDYPNGQLATVLGLPTAAGNWWDYDMMAVAGGSWSNQAFVPGNGQLIQASSSYTAIV
                     MCLVEPHTEAVTIELTSTAQDAAKSLSNDGRPSAVAKKGELIPLTVTVRDSAGNPMPY
                     ADFTLSRETTLDRAKATVNTSADDLTVTALVPVSRDSVLTASGAKTLATTGSDGTATF
                     EVGQYSTTGLATPLTVTLARDTTKVATLDVIYTVITSPDSPSAKFWGHMPDTFTSSAG
                     VTFKRPLLKTETSSGLSISSNGEVWSYMSNLQNLTSTDCPLENQPRSKELLDLYSDHP
                     NGAIMTDLGLPMNAGNWWAYDMAILGTTTWSYQTVSLRTGAIAKNSATNALMLCLAQP
                     HPAGVSVTLTSSALDDARTLSNGGRPSASVKKDNPLPIVVMVKDRDGNPLAGEAITLR
                     RDNAMSRSGTIVNTAANVMVLTELAPVSATFTLASHGTQWLGFTGSDGTAIFNVEQPD
                     TVGLATPFTAILARDTETTTVLDAIFTVVTSPDSAMAAYWGHMPDTVTAENGVVFERP
                     KLWKELPSTSGVSKVNSNNEVWPVFNATQRADSNLSPCEAARRPLSDDLESLYARYPG
                     NTLTTQIGWSTQYTWWALDNWLGGSNPQVVGLNNGSAWGTVNTAYQGCLVNPRATVSS
                     VTLTSTALDSSTQAAKVKKGDAVPVVVTVKDSVGKPVPNISFILKRGDATPRNSGATL
                     YGDVDTMDDLTVQPSSGAAVTLADSGNTIDGVTGADGTASFTVGQDNTPGYKTPLTVT
                     LTDNATITATLDTIFTVPTSPNVATAYFWGHMADTATVSGKMLHRPLLKSELPSGVTA
                     AATPNVNNETWALAHVIDSTKWDVARQCGSMNNVPSSAELQTLHSGFSTLGWPSSISF
                     PYLSTDKAGSFYCGVEEGSGSLNCGIQPAKTPGFATCFQ"
     gene            complement(14105..15097)
                     /locus_tag="ISB30_003183"
     CDS             complement(14105..15097)
                     /locus_tag="ISB30_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990787.1"
                     /translation="MKPHMKRGLTKVALALMLAGYCAVPVAMAEDAAWVASGTTAEFE
                     GTIPWLYREGGNATINSEDADHIKVTSDSKGIRPAGSESDKRLYSGDTITLGWDIGDT
                     EGDIDDGSAGIDAKTTATIKWYSYSDNAGGGKTELTAAAGKTSYKITDGDRGRYIGVE
                     IQPITQTGNPFQGTSLTLLDISTASGGGSDTDNVDPGPVVNQNLKVAIFEKDTSTNLI
                     GGNTAIALNKTYVAKLYSDENQNGKYDAGTDVDVTANYDFAWVFNGNSKQLAAAGGIA
                     NASFDNNDIVIPQTNEQARTSLNGSDRNGKTGLAIPANGDGVQGYTLSIIYKHH"
     gene            complement(15153..>17326)
                     /gene="sinH"
                     /locus_tag="ISB30_003184"
     CDS             complement(15153..>17326)
                     /gene="sinH"
                     /locus_tag="ISB30_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HAP1990788.1"
                     /translation="LRWKRCIMLTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQT
                     NKGKSLKERGADYVINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPYG
                     KVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRHNF
                     DKWLLGGNIFYDYDFTRGHRRLGIGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEER
                     PASGWDIRAEAWLPAYPQLGGKIVFEHYYGDEVALFGTDNLEKDPFAVTLGVKYQPVP
                     LIAVGTDFKAGTGDNTDTTVNATLNYQFGVPLKDQLDPDKVSAAHSLMGSRHDFVERN
                     NFIVLEYKEKDPLDVTLWLKADAMNEHPECVIKDTPEEAIGLEKCKWTINALVNHHYK
                     IVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPVWQYSSDKAEQGKLNTWRV
                     RLALKDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGVV
                     MDLDITDSFGDNTDRNGNVLPQDNLNPQLFDANDKKVTLTNNPCTTETPCVFIAKQDK
                     EKGTVTLSSTLPGTFRWKAKAAPYDDSNYVDVTFLGADIGGLNAFIYRVGAAKSVNLI
                     GNKEPLPLNSRYRFVLWRDANKDGVFQLSEKLTDEEMKQYDYQWEFTGHSVNGNTGAQ
                     ANTTNVDIEIPATNKEAAAKFSAQVTDGVQGYGLQVNYSKT"
BASE COUNT         3923 a         4726 c         4397 g         4280 t
ORIGIN      
        1 cttttgatga tcttaaatgt cttatttttc gtaatgtgta taacaaggaa tagtgatgaa
       61 atttaaaaaa tgtcttctgc ctgtggcaat gttagcgtca ttcactctgg caggatgcca
      121 gtcaaatgct gatgatcatg ccgccgatgt ttatcaaacc gatcaactga ataccaaaca
      181 agaaactaaa accgttaata ttatttccat tcttcccgca aaagttgccg tagacaacgc
      241 ccaaaataaa cggaacgcac aagccttcgg cgcgcttatt ggcgctgtcg ctggcggtgt
      301 tatcggccac aacgtcgggt ctggcagcaa ttccggaacg acggcaggtg cagttggcgg
      361 cggagctgta ggcgcggcag cgggttctat ggtgaatgat aaaaccttag tggaaggtgt
      421 ttctttaaca tataaggaag gcaccaaagt gtatacctcc acccaagtgg gtaaagagtg
      481 ccagtttacg acaggtttag ccgttgttat taccacgacg tataacgaaa cgcgtattca
      541 gccaaatacc aaatgtcctg aaaagagcta ataatcagga ggagtcatga agaaagtttt
      601 tctttgcgcc atcttagcct ccttaagcta tccggctatc gcctcatcat tgcaggatca
      661 actctcggct gtcgcagaag cggaacagca aggtaaaaat gaagagcaaa ggcagcatga
      721 cgaatgggtc gcggagcgca acagggaaat ccagcaagag aagcaacgtc gcgcaaacgc
      781 ccaggccgcg gctaataaaa gagcggcaac ggcagcagcg aataagaaag ctcgtcagga
      841 taaactggac gccgaagcca ctgcggacaa aaaacgcgat caaagttatg aagatgagct
      901 acgtagctta gagattcaga aacaaaaact ggcgctggcg aaagaagaag cccgcgttaa
      961 gcgcgaaaac gaatttatcg atcaggaact gaagcacaaa gctgcgcaaa ccgatgtggt
     1021 gcaatctgaa gctgacgcaa acagaaatat gactgaaggc ggtcgcgatc tgatgaaaag
     1081 cgtgggtaaa gcagaagaga ataaatcaga tagctggttt aactaaacga tgttagtaac
     1141 ttcaaaccta taattcttta ggataaaaaa ccctctgtag taacagaggg ttttgttcat
     1201 tcatagtgca gggtcaaatc attcccactc aatagttgct ggtggcttgc cgctgatgtc
     1261 atacaccacg cgggaaatac cattcacttc attgataatg cggttggaaa ctcgaccgag
     1321 gaaatcgtac ggcagatgtg cccagtgtgc ggtcataaag tcgatggttt cgacagcacg
     1381 cagagagaca acccagtcat acttacgacc atcgcccatt acgccaacgg aacgtactgg
     1441 caggaagacg gtgaacgcct ggctgacttt gtcgtacagg tccgctttac gcagttcttc
     1501 aatgaagatg gcgtcagcac ggcgcagcag gtcacaatac tctttcttca cttcccccag
     1561 aacacgaacg ccaaggcctg gtcccgggaa cgggtgacgg tacaacatgt catacggcag
     1621 gcccagctcc agaccaatct tacgcacttc gtctttgaac agctctttca gcggttcaac
     1681 caggcccatc ttcatctctt tcggcagccc acccacgttg tggtgagatt tgatgacgtg
     1741 tgctttaccg gttgcagaag cagcagattc gataacgtca gggtagatgg tgccctgcgc
     1801 cagccacttc acgtcttcca gtttcagcgc ttcttcatcg aatacttcaa cgaaaacgcg
     1861 cccgatgatt ttacgttttg cttccggatc gttttcgcca gccagcgctg acaagaagcg
     1921 atcttctgcc ggtacgtgaa caatgttcag accaaagtga tcgccaaaca tatccagaac
     1981 ctgctctgct tcgttgagac gcagcaggcc gttgtcgacg aatacgcaag tcaggttttt
     2041 accgatagcg cggtgcaaca gcattgcggt tacggaggaa tccacgccac cagagaggcc
     2101 gaggatgact ttatcgtcgc ctacctgctc gcggatgcgg gctacagcgt cgtcgataat
     2161 tttcgctggc gtccacaggg cttcacactg gcagatatca cgcacaaaac gctccagcat
     2221 gcgcataccc tggcgggtgt gagtcacttc cgggtggaac tgtacgccat agaagcgttt
     2281 ttcttcgttg gccatgatgg caaacgggca gctttcggtg ctggctacgg tgacgaagtc
     2341 ggacgggata gcggtaactt tatcgccgtg gctcatccag acgtccagca gcggtttacc
     2401 atctgcggtc agagcatctt cgataccgcg aaccagtgcg ctgtcgttta cgacttcaac
     2461 ctgcgcgtag ccaaattcac gttcattaga agcctcaacg tgaccgccca actgcatcgc
     2521 catggtctgc ataccatagc aaacgccgaa taccggtacg cctgcttcaa agacatactg
     2581 cggcgcacgc gggctgtttt cttcagtagt actttccggg ccgccggaaa gaataatgcc
     2641 gcttggattg aagtcacgaa tttgtgcttc tgtcacatcc cacgcccaca gttcgcagta
     2701 aacccccagt tcacgcacgc ggcgcgcaac cagttgagta tactgggaac cgaagtccag
     2761 aatgaggatg cgatgcttat gaatgttttc cgtcattgac gcttattccg aggcaagtga
     2821 aacagataat ataaatcgcc cgacatgaag tcgggcgaag agaatcagga gcccagacgg
     2881 tagttcgggg actctttagt aatggtcacg tcgtgaacgt ggctttcctg aatacccgca
     2941 ccgctgatac gtacaaactc cgctttagta cgcagttcgt cgatagtacc acagccggtc
     3001 aggcccatac aggagcgcag gccgcccatc tgctggtgaa tgatctcttt caggcgacct
     3061 ttataggcta cgcgaccttc gataccttcc ggcaccagtt tgtcggcagc gttgtcgctc
     3121 tggaagtaac ggtcagaaga acctttggac atcgcgccca gggaacccat accacggtaa
     3181 gatttgtaag aacggccctg gtagagttcg atttcacccg gagattcttc agtacctgcc
     3241 agcatggaac ctaccatcac cgcgcttgcg ccagcggcga tagctttagc gatatcgcct
     3301 gagaaacgga taccgccgtc agcgataacc ggaatgccgg taccttccag ggcttctact
     3361 gcatcagcaa cggcggtgat ctgcggaaca ccgacaccag tcacgatacg cgtcgtacag
     3421 atagaaccag ggccgatacc cactttaacg gcactgcaac cggcttctgc cagcgcgcgg
     3481 gcacctgcag cagttgccac gttgccgccg atgatttgca ggtcaggata tttagcgcga
     3541 gtttcacgga tacgctgcag aacaccttca gagtgaccgt gagaggagtc gatcagcaga
     3601 acgtcaacgc ctgcggcaac cagcgcatca acacgctctt cgttaccagc gcccgcgcca
     3661 accgctgcac ctacacgcag acggccttgt tcgtctttac acgcgttcgg tttacgttcc
     3721 gctttctgga agtccttaac agtgatcatg ccgatcaggt ggaattcatc atcaaccacc
     3781 agcgcttttt caacgcgttt ttcgtgcatt tttgccagca ccacttcacg ggcttcgcct
     3841 tcacgcacgg ttaccagacg ctctttcggc gtcatgtaaa cgctaaccgg ctggctcaag
     3901 tcggtcacaa aacgcacgtc acgaccggtg ataatgccga ccagttcgtt ttcttcggta
     3961 acaaccgggt agccagcaaa gccgttacgc tcggtcagtt ctttcacttc acgcagcgtc
     4021 gtagttggca gtacggtctg cggatcggtt accacgccag attcgtgttt tttcacacgg
     4081 cgaacttctt ctgcctggcg ttcaatggac atgtttttgt ggataaagcc gataccgcct
     4141 tcctgagcca gagcaatagc caggcgcgct tccgttacgg tatccattgc tgcggaaagc
     4201 atagggatat tcagacgaat agttttcgtc agctgagtgc tgaggtcagc agtatttggc
     4261 agaacggtag aatgagcagg aacgaggaga acgtcgtcaa acgtcagagc ttctttagcg
     4321 atacgtagca tgggcaatat ctcgaccaga gtggttaata aatattgccg cggcattata
     4381 cagagcgtaa ccgattgcat ctaccccttt ttgcaaaaaa tgcttgctat ccccggaggg
     4441 cgggttacta tcgactgaat aacctgctga tttagaattt gatctcgctc acatgttacc
     4501 ttctcaatcc cctgcaattt ttaccgttag ccgcctgaat caaacggttc gtctgctgct
     4561 tgagcatgag atggggcagg tgtggatcag cggcgaaatc tctaatttta cacaaccagc
     4621 ctccggtcac tggtacttta cgcttaaaga cgataccgcg caggtgcgct gtgcgatgtt
     4681 ccgcaacagc aaccgccggg tcaccttcca cccacagcac gggcaacagg ttttagttcg
     4741 cgccaatatt acactctacg agccgcgcgg cgattatcaa attatcgtcg aaagtatgca
     4801 gccggccggt gagggattgc tgcaacagaa gtacgaacag cttaaagcga agttgcaggc
     4861 tgaaggtttg ttcgatctgc aatacaaaaa atcactcccc tcccctgcgc attgcgttgg
     4921 tgtgatcacc tcaaaaaccg gtgctacact acatgatatt ttgcatgtgt taaaacgtcg
     4981 cgatccttct ctgccggtga tcatctaccc caccgccgta cagggggatg atgcgccggg
     5041 gcaaatcgtt cgtgccattg aactggcgaa taagcgcaac gagtgtgacg tgttgatcgt
     5101 tggccgtggc ggcggttcgc tggaagattt atggagcttt aacgacgaac gcgtggcgcg
     5161 ggcgattttt gccagtcgca ttccgatagt cagcgccgtc gggcatgaaa ccgatgtgac
     5221 gattgccgac tttgttgctg atctgcgtgc gccaacgccg tcagccgccg ctgaagtggt
     5281 gagccgaaat cagcaagaat tgctacgtca ggtgcaatct gcccgtcaac ggctggaaat
     5341 ggcgatggac tattatctcg ccaatcgcac gcggcgcttt acgcagatcc atcaccgctt
     5401 gcagcaacag catccgcagc tccggctggc acgccagcaa actatgctcg aacgtctgca
     5461 aaagcggatg agctttgcgc tggagaatca gcttaaacgc gcgggtcagc aacagcagcg
     5521 attaacacgg caactcgtcc accaaaatcc gcagtcgcgt attcatcgcg cgcaaacgcg
     5581 cattcagcaa ctggaatatc gtttagcaga aaccctgcgc gcacagctta gcgccacgcg
     5641 cgaacggttt ggtaacgcgg taacacacct cgaagcggtt agcccgctgt caacgctggc
     5701 gcgcggttat agcgtcacca gcgccgccga tggcgcagtg ttgaagcagg tgaagcaggt
     5761 gaaagtgggc gatacgttga ccacgcgact gggagatggc gtggtcgtca gtgaagtgag
     5821 tgcggtgacg aaaacccgca agccgcgtaa aaaaacctga tagcccgttg cgtatcaggc
     5881 tttcggaacc tcatattgat agggtgggca aggttattgg aaacaggttg caaaccccgg
     5941 cgttttcgca ggctgaatgc cacagttgag actgccagag ccttcctcaa caccacaata
     6001 gaacgatcca gccttatcag tagataaata cgggaagctt atcgatgacg gccatcccaa
     6061 tgtgctaaag ccggagtgta atgtttgtaa ttcggcactg ctcggcacgt tattcatact
     6121 accgcactgc cgtgcgacat cccatttagt ggaatctata acatgtgcca gcgcccaggt
     6181 ttcattattc acattgggcg ttgctgctgc ggtaacccct gacggcaact ccgattttaa
     6241 tagcgggcga tgcagcatct tgccacttac cgtggcggta tccgccatat gaccccagaa
     6301 atacgccgtg gcgacattag gactggtcgg cacggtgaaa atcgtgtcca gcgtcgccgt
     6361 tattgtggcg ttatccgtca gcgtcaccgt caacggcgtt ttatatcctg gcgtattatc
     6421 ctgaccaacg gtaaaacttg ctgtaccgtc tgctccagta actccatcga ttgtattccc
     6481 gctatcagcc aacgtgactg ccgcaccgga tgagggttgt acagtcaaat cgtccattgt
     6541 atccacatcg ccatacaacg tcgccccgct attacgcggc gtagcgtcac cacgtttgag
     6601 gataaacgaa atattcggca ctggtttccc gacactgtct ttcacggtaa cgacaacggg
     6661 aacggcgtcg ccttttttca ccttcgccgc ctgcgtactg ctatccagtg ccgtcgatgt
     6721 caacgtgacg ctggagacag tcgcacgcgg gttaaccagg cacccttgat acgcggtatt
     6781 caccgtcccc cacgctgaac cattattcag accgactacc tgcggattac tgcctcccag
     6841 ccagttatcc agcgcccacc atgtatactg cgtagaccaa ccgatttgtg tcgtcagcgt
     6901 gttccccgga tagcgagcat agagcgattc taaatcatca ctcaatggcc gacgtgccgc
     6961 ttcacaaggg ctgagattgc tgtcagccct ctgcgttgcg ttaaacacgg gccagacttc
     7021 attgttacta ttgaccttgc taacccctga tgtcgacggc agctctttcc acaatttagg
     7081 tctttcaaag accacaccgt tttctgccgt taccgtatcc ggcatatgac cccagtaggc
     7141 cgccattgcg gaatcagggc tggtgacaac ggtgaagatc gcatcaagga cagtggtggt
     7201 ttccgtatca cgtgccagga tggcggtaaa cggtgttgcc aggccgacag tatcaggctg
     7261 ttcaacattg aatatcgccg taccatcgct gccggtaaaa ccaagccatt gcgtgccgtg
     7321 tgaggcgaga gtgaatgttg ccgacacggg tgccagttcc gtcaatacca tcacatttgc
     7381 tgccgtgttt acaatggttc ctgagcggct catcgcatta tcacgcctga gcgttatcgc
     7441 ttcgcccgcc agcgggttgc catcacgatc tttgaccata accacaattg gcaatgggtt
     7501 atcttttttc actgacgcgc taggtcggcc gccattgctg agggttctgg cgtcatccag
     7561 tgcggatgac gtcaatgtta cgctaacgcc tgccggatgc ggctgcgcca gacagagcat
     7621 cagtgcgttg gttgcagaat tcttcgcgat agctcctgtt ctcaggctga cagtctgata
     7681 agaccacgtt gttgtaccca gtatggccat atcataagcc caccagttac ccgcattcat
     7741 cggtaaccca aggtcagtca ttattgcgcc gttcggatga tcactataca aatccaacag
     7801 ttccttactg cgcggttgat tctcgagtgg gcaatctgta gatgttaaat tttgcaagtt
     7861 gctcatatac gaccaaactt cgccgttaga actaatcgat aagcctgagc tggtttcggt
     7921 tttcagcagg ggtcgtttga aggtgacgcc cgccgaactg gtaaaggtgt ccggcatatg
     7981 cccccagaat ttagcactcg gtgaatctgg gctggtaatc accgtgtaga taacatccag
     8041 cgtggcgact ttcgttgtat cacgcgccag cgtcacggtt aacggcgtcg ccaacccggt
     8101 agtagagtac tgcccaacct caaaggtggc tgtgccatca ctgcccgtcg ttgcgagagt
     8161 ttttgctcca cttgctgtca acacgctatc cctactcacc ggaaccaatg ccgttacggt
     8221 aagatcgtct gcactcgtgt taacggttgc tttcgcgcga tccagcgtcg tttcacgcga
     8281 aagggtaaag tcggcatatg gcatcgggtt cccggcgcta tcacgcacgg tcaccgtcaa
     8341 cggaatcagc tcacctttct tcgctacagc gctcggtcgt ccatcattgc tgagcgattt
     8401 tgctgcatcc tgcgccgttg aggtaagctc tattgtgact gcttccgtat gaggctctac
     8461 caaacacatc actatcgccg tatagctgga cgatgcctga atcaactgac catttcccgg
     8521 aacaaacgcc tggttactcc agctaccgcc cgctaccgcc atcatgtcat aatcccacca
     8581 gttccctgca gcagtcggca agccgagcac cgttgccagt tgaccattgg gataatcgac
     8641 atacagcccc tgcatatcag tcgtcagcgg ttgatacgcc tcatcacacc cagccttgct
     8701 gacgtccgct ttctcagtcg cggtcaggag cgaccagact tcattattgg ccgttttcgc
     8761 cgtaccgcca gagagttcgg ctttgaggaa aggccgtttg aaggtgacgc cttcgctgct
     8821 ggtaaaggtt tccgccatat ggccccaatt ggtcgccagc ggcgtatccg ggctggtggt
     8881 gacggtaaag atcaccgaca aggtgtccga cgacacgcca gtatcgttta atagagcata
     8941 aaagtcggtt cttaacccag tggaagcatc ctgtcggagc gtcatcgtag tggttccatc
     9001 tggccctgtt acaccataca ggatggtcgt tgcactggta tccaccgtcg cacccgccgc
     9061 agtcgtgact gacactttct gcgatgacgt cacattctga cgattgatcg agttcgcgtg
     9121 tttcagggca aacggcgtat tgccgactaa attcccctgc gcatctttag tcgaaatgcg
     9181 gatcgacacc tcatccccct tcttcgcttt gacggcgcta agtgtgctgt caatgctgac
     9241 aaactgcccg gggtctgagg cttccagcgt aatctggctg gcagtcatca acggcgttgt
     9301 acggcaggtt tgtaacgcca gcacggtgga tacgatttgc gaaactgcgc cagaatttag
     9361 ttgcaccaca ttgtagtaac ggctccctgt ttgtgtatta ctcggggtat tggtgagata
     9421 actcatattt accggccatc cctgtaccgt attcatcgca ttacctctat tggcgtcata
     9481 caaagacttc agaccatcca gcgttggcat ataatctgta ccacacccat tcatgctggt
     9541 gtcagccgac tgagtcgagg tgaatcttgc ccaactttca ccgttctcaa acgtgctata
     9601 tcgtgcatag ttgttggtaa gctcgccaaa cagcaacggt cgcttataaa ccgaaccatc
     9661 ggaggccgtc accgtttcgg ccatatgtcc ccaaaaattc gcttttggcg aatccgggct
     9721 ggtcacgacg gtaaagacca ccggtagcat cgccttgacg ctgctggacg tatccagttt
     9781 tgccgtgaga tcggttctta acccggtact gttatcctgt tttagggtaa atgtcgttga
     9841 cccgtcggca ccggtcacgc cgtaaatggt ttcactcgtt gactggaaat tatttcgcgt
     9901 atttccccac gcatccgtca ccgtcagcgc gcccacactc atcgtcgccg atgaattagc
     9961 ccgacttaca ctgttggctc gcgtcaggat aaatgccgta ttgcccacaa ggttgccttg
    10021 cgcatctttg gtgatgacgc gcaccacgga ttcttcgctt tttttcgctt ttaccacgtt
    10081 ataggtggta tccagcgtca ccggatctgc cgcttccagc aggatttggc tcaccgcggc
    10141 tatcggcgcg gtctggcagg tcagcagctc cagcgtcgtt gatgacactg ccgcactcgt
    10201 gccattgctg agatctaccg ccttgtagtt acgctgctcc atgctgccgt tgtcggaagt
    10261 gttggataaa tagcgcgttg ccaccggcca gccctgcacg gttttcatgg tgttgcccgc
    10321 attattagca aacagtgacg tcagaccggc ctgagtcgga atatagttgg ttccacagcc
    10381 gctgtacgtc gtggaaaaac ttgcataatt aatgttaaac aacgcccagt tttcattgtc
    10441 ttcagacgtt gatgttcgcc cagtctgata ggccagttcc ttcaacaaca gcgggcgctt
    10501 aaataccgta ccgtccgcag cagtcaccgt ttccggcata tgtccccaca tttttgccag
    10561 gcggctatcc gggctggtca ccaccgtaaa gattgtgtcg atgctggatt ttttcgtcgc
    10621 atcagagtaa agcattgccg tcagcgccgt tttggtgccg tgagtatccg ggcgggtaat
    10681 attgagaatt ttgctgccgt cattgtcggt catggcggta tacaccgttg cagtgtcgtt
    10741 caacgtggtt tcatctgcca gcccgccatt gactaccacc ggcgcaacca gcgcatcgcc
    10801 gctgcccgcc acatgttttt cgccgctacg ggtatagcca tcaccgcgat tgagggtaaa
    10861 tggcatatcc gctaacgcgt tgccctgtgc gtcttttacc gtcactttaa cctgtagcgt
    10921 ctcgcctttt ttcagcgttg ccgcgctatt cccggtattc cattgcgctt tatcaaccac
    10981 ctccagcgta atgctgctgg caggtttgtt cgccgtcttc aggcaactga cgtaaaccgg
    11041 cgtatcgccg ccaatagttt gtgcgccatc gttcagcgct atcgtgaaaa agtgtggagt
    11101 tttatctgcc ggtgagttgc tccagtaagg ctgacgctgt gttggccaac cgtgcgccgt
    11161 ttgcaccgcg ttaccactgt tggcgttata cagcgcactt agctgggaac gacgtggcaa
    11221 catattggtg tcgcagccac ctttactggt actggacgta ttggcctggc tcacgcgcgc
    11281 ccagatttcg ttattttcgg tcagggttcc gtccgggctg gcgacttccg acgccagttt
    11341 aggccgggta aaggttgatg aatcaacggt gatcgtttca tccatatggc cccacatctg
    11401 cgcgccggta acgtccgggc tggttggggt agtaaaaata acgctgtagt taacggtatt
    11461 cggcaggtag gaatttaccg gcgttacgct cagcaccgtt tttagcccga cgccctgtgg
    11521 ttgcttaatc tcgaccgtcg ccacgccccg ggcatcggtt attcccgcat aaaccttcga
    11581 tgactgtgat gtgccgtatt gcgtgttatc catcgtgatt gcaccactgc tcgggtcatc
    11641 aaacccgctc acctgccccg ctcggttata ccccatacct ttggtaatgg tgaatgcagt
    11701 aaagggtaca gggatattat tgagcgagtt aatcgtacgc acggtcatca caatggtgtc
    11761 acccactttg gcttgcgctt gggtgccaac agacacatca tgatcggtgc tgacttcaac
    11821 attcgctacc atctcattac ccgaacagga gaggtaattc ggcttaaagc cggaatagct
    11881 gtcgacgctc ccgttcccca gatccaccga tgaatgcgtg gcttgctcca ccgcgcttaa
    11941 atagccctgt tgtgcagtgg gccagccata ttcagtacca atcgcgttac cgggatgggc
    12001 tgaaaacagg ctttccagtg agctctggcg cggtatatga ccgacgccgc attccgcctg
    12061 catactggtg ttttgatcgt agagcgccca gtcttcgtta ttttcccgca ctgttcccaa
    12121 ttcatgctcg gtttcatccg ccagcaacgg gcgtttatac aaactgccgg actcaataat
    12181 gccgcgcata tggccccaca tccgcgcttt gtcgctgtcc gggctggtga ttacggtaaa
    12241 aatcacatct ttggcatctg tggcgttaaa atcgctgcgc attcttgccg tgatatgggt
    12301 tttcaccccc gcgccgccgg gctggctcag ggtgagcgaa gcctggccgt tggcgtcggt
    12361 catgccttca taggtatgtg cgtcgacctg gcgaaaatta ctgccgccag caatctgtac
    12421 cggatgggct tcccaggcag tgtcggtttg attcttacga ttgacgccat catccagatg
    12481 tagcgagaag tagtaatacg ccagcggttg atcgttgttt ttcgtgtcgg tgatggccag
    12541 acgcatcgtc gcatcctcgc ccaccttcgc tttcatcgcc tgcgccgtgt catcatagtt
    12601 ttctggcgtg aggacgattt tcggctcgac gtcaggcgcg ggcttatcca cgcagctcac
    12661 gaggaacgtg gtgctgtccg gcttctgcgt ttcgccacgg ttagagacat ctgccgcatg
    12721 gtgctgcccg gtaagccccg ccagcgatga ccagtaataa tcgccctgca tcggccagcc
    12781 gagcacagtc tgtaccgtgg agggaatcac cgtcgccagt gcgccgaagt ggcgcatgcc
    12841 cggcagcacg gtgcaatggc tatctgcgcc gctccaggta aaggtggacc aggtttcatt
    12901 gtgatcgacg accgtggcat tttcgttact gacttccgcc gccagttttg gtctactgaa
    12961 cgtgtaacca tgggcttcca ccgtctccgc catatggccc cacatcgtgg cctgcgccac
    13021 gtccgggctg gtgagcacgg taaagatcac cgccgcctca ctggtttgtg caatgccgga
    13081 aatgccgact accagcggcg ttttaacacc ggggccgtta ggctgggtca ccgtgatggt
    13141 tgccgtaccg ttagcatccg atgtgccacg atactcggtt accgtcgtcg ttaactcggt
    13201 gctatccagc accaccggcg cggtattatt taccgcgccc tgacggtttt gggcatcttt
    13261 acgcttgata ataaatggaa tattacccgc gacgttaccc tgacaatctt tggtggtgac
    13321 ggtcagcgtg atagtgccgc caacggtggt ttttgccgcg ccgagcgttt cgttataacc
    13381 actggcgctc accgaaatta aatcgccctg cgcgggcgcg atattcagcg tcactttctg
    13441 gtcagccagc accaccgtcc cctcataggg ataaccggtc gaggtcagca gataaagcag
    13501 tggaatggtc tgctgcgcat caatctgtgg ccaggccacc agcgaacgcc cggccagacc
    13561 ttgcgcgcaa aagctgcggt tggcgatggg ctgtggattg agcggcgtat ccggcgtggc
    13621 ggcatccgcc cacaccagac tgacggtcgg cggcgtctgg ttttcccagc gcagcgcagg
    13681 tggtgtagaa aactggtcgc cttcactgtc ctgaggatta gcaactatca ggttagcagc
    13741 gtcatcgtca acgctaaatt cattcggttt agcagaaaac gccacatcat gagatgcagc
    13801 aggatcaagc tgaacgcttc cctgataaac gggtacgctg gcgctatcgg caataggaac
    13861 tgtaccgtta aactcgcctg tagaaccagg atatgtctgc cattccacct ctgcccgtgc
    13921 aaagtcagac agcataaagg ctgccaacat gcccagaagc acaatgattt ttcctggttt
    13981 cagacatacc gacatgtaga aaactccagc atgataaaca aaacgtcaac ccgttttgct
    14041 taacgcgtcg ctaagcaaaa caggcggaaa acagcattcc catacgggaa tgctgttgtg
    14101 cgcattagtg gtgtttgtaa atgatagaca gcgtatagcc ctgaacgccg tcgccgtttg
    14161 ccgggattgc aaggccggtc ttaccattac ggtcactacc gttaaggctg gttcttgctt
    14221 gttcgttagt ttgcggaata acaatgtcat tgttatcgaa gctggcgttg gcaataccgc
    14281 ccgccgccgc aagttgttta ctgttaccgt taaagaccca cgcaaagtcg tagttagcgg
    14341 taacgtccac atccgtaccc gcgtcgtatt taccgttctg gttttcatcc gagtacagtt
    14401 tggccacata cgttttgtta agcgcaatgg ctgtattacc accgataagg ttggtactgg
    14461 tgtctttctc gaagatggca actttcaggt tctggttaac aaccggacca ggatcgacat
    14521 tatccgtatc gctgccgcca ccgctggccg tagaaatatc cagcagagtc agcgaagttc
    14581 cctggaaggg attaccggtc tgagtgatag gctggatttc aacaccgata tagcgtccac
    14641 gatcgccatc ggtgatttta taactggttt ttcctgccgc cgcagtcaac tccgttttac
    14701 cgccacccgc attatcactg tagctgtacc atttgatggt cgccgtcgtt ttcgcatcaa
    14761 tgcctgcgga accatcatcg atatcccctt cggtatcgcc gatatcccaa cccagcgtga
    14821 tggtatcccc tgagtaaagg cgtttatcag actcgctacc tgcaggacgg atacctttac
    14881 tatcagatgt cactttgatg tgatccgcat cttccgagtt aatcgtagca ttcccccctt
    14941 cgcgatagag ccagggtatg gtcccctcga attcagctgt cgttccactg gcgacccagg
    15001 cagcatcttc cgccatagcg actggtacgg cacagtatcc agccagcatc agtgccagcg
    15061 cgacttttgt caggccacgt ttcatatgag gtttcataaa cactctcctt ggttaacatt
    15121 cagcggtggc ccgatcagcc atggcgctgt tcctatgtct tgctgtaatt gacctgcaaa
    15181 ccgtaccctt gcacaccatc ggttacctgg gcactaaact tcgctgccgc ttccttgttc
    15241 gttgccggga tctcgatatc gacgttagtg gtattggctt gcgcgccagt attgccattt
    15301 acgctatgcc ccgtgaattc ccattggtag tcatactgtt tcatctcttc gtcagtgagc
    15361 ttttcagata gctggaacac accgtctttg ttagcgtcgc gccacaacac aaagcgatat
    15421 cggctattga ggggcaacgg ttctttattg ccgataagat tcacggattt agccgcacca
    15481 acgcgataga taaaggcatt cagcccgccg atgtcagccc ccaggaacgt tacgtcaaca
    15541 tagttactgt catcataggg ggcagctttc gccttccaac ggaaggtgcc aggcaacgta
    15601 ctggagagcg taacggtccc cttttcttta tcttgtttgg cgataaagac acagggagtc
    15661 tcggtagtgc aggggttatt cgtcagcgtc acttttttat cgttggcatc aaacagctgc
    15721 gggttgagat tatcctgcgg caagacatta ccgttgcggt cagtgttgtc gccaaagcta
    15781 tcagtaatat cgagatccat caccacacca tctacaccat cagcctgtgc actggcttcg
    15841 tggctgtggt tgttctcttc cggcacgttc gcgatgttat taacaatcag ctcaatttta
    15901 cggtcctgct gaacggtgat ttccaccacg cctgagttct gtcggttgcc cttttcatct
    15961 ttcagcgcca ggcgaacccg ccaggtattg agttttcctt gttcggcttt gtcagagctg
    16021 tactgccaga cgggcagcac caggttccag tggttattgt tgccctctgt cagggtattc
    16081 tctttgataa ccggcatcac cagcgtgcgg gcggcattgt ttttcgcctg ccatgacgca
    16141 gcgacaattt tgtagtggtg gttgacaagc gcgttaatgg tccatttaca tttttccagc
    16201 ccgatagctt cttctggcgt atctttaatc acgcactcgg gatgttcatt catcgcatca
    16261 gctttcagcc acaaagtgac atcgagggga tctttctctt tatattccag aacaataaag
    16321 ttgttacgtt caacaaagtc gtgacggctg cccatgagtg agtgagccgc gctcactttg
    16381 tcaggatcaa gctggtcttt gagcgggacg ccaaactggt agttaagcgt tgcgttgacc
    16441 gtggtatcgg tgttatcacc ggttcctgct ttgaagtccg ttcctacagc aatgagcgga
    16501 acaggctggt atttcacgcc taacgtgaca gcgaagggat ctttctctag attgtcggta
    16561 ccaaatagcg caacttcatc cccgtaataa tgttcgaaaa cgattttacc gccaagctgc
    16621 ggataagcgg gaagccaggc ttcggcacga atatcccaac cgctcgcagg gcgttcttca
    16681 taaaagtcga aatcttcaga gtctttccag tcagaaagcg gatgataata gttaccagaa
    16741 aattttaaat aatcagtcca cgcttcagcg cctataccta aacggcgatg tccacgggta
    16801 aaatcataat cataaaatat atttccaccc aataaccatt tatcaaaatt atgtcttaca
    16861 ccaagcccta tatttccgat cgtacgatct tcttttcgtt gcgcagaaaa ttggctgaaa
    16921 taaacagtgg tttgattatc gtaccaggga acaaaataat cgatggaact gccatccaga
    16981 tcgccgccct gaccaatgga gaggttcgaa cggaccttac cgtagggaga gagtgtgtct
    17041 tcaatataag attgtgcggt tgaggtgatt tgactttgca gatagcttct ggcctgagat
    17101 tccagcgcct caggggtcaa gttttcaaac ccttgcgtgg cggagttgat gacgtaatcg
    17161 gctccgcgct ctttcagcga tttacccttg ttggtttgtt catcctgttg tgctgcctgg
    17221 ctacctaaat caggtaacga ttcgttttgc ttaacattta tctctggtgc cgcgaaagca
    17281 gcaccagaga taaatgttag cataatacag cgtttccatc gcagca
//
LOCUS       DACADF010000099        17286 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.101, whole genome shotgun sequence.
ACCESSION   DACADF010000099 DACADF010000000
VERSION     DACADF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.101"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(37..768)
                     /gene="rlmB"
                     /locus_tag="ISB30_003185"
     CDS             complement(37..768)
                     /gene="rlmB"
                     /locus_tag="ISB30_003185"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP1990789.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(859..3300)
                     /gene="rnr"
                     /locus_tag="ISB30_003186"
     CDS             complement(859..3300)
                     /gene="rnr"
                     /locus_tag="ISB30_003186"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP1990790.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3339..3764)
                     /gene="nsrR"
                     /locus_tag="ISB30_003187"
     CDS             complement(3339..3764)
                     /gene="nsrR"
                     /locus_tag="ISB30_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAP1990791.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3969..5267)
                     /gene="purA"
                     /locus_tag="ISB30_003188"
     CDS             complement(3969..5267)
                     /gene="purA"
                     /locus_tag="ISB30_003188"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP1990792.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGLEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5371..5568)
                     /locus_tag="ISB30_003189"
     CDS             complement(5371..5568)
                     /locus_tag="ISB30_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAP1990793.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5650..6654)
                     /gene="hflC"
                     /locus_tag="ISB30_003190"
     CDS             complement(5650..6654)
                     /gene="hflC"
                     /locus_tag="ISB30_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAP1990794.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6657..7916)
                     /gene="hflK"
                     /locus_tag="ISB30_003191"
     CDS             complement(6657..7916)
                     /gene="hflK"
                     /locus_tag="ISB30_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAP1990795.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8002..9282)
                     /gene="hflX"
                     /locus_tag="ISB30_003192"
     CDS             complement(8002..9282)
                     /gene="hflX"
                     /locus_tag="ISB30_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP1990796.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9358..9666)
                     /gene="hfq"
                     /locus_tag="ISB30_003193"
     CDS             complement(9358..9666)
                     /gene="hfq"
                     /locus_tag="ISB30_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAP1990797.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9752..10702)
                     /gene="miaA"
                     /locus_tag="ISB30_003194"
     CDS             complement(9752..10702)
                     /gene="miaA"
                     /locus_tag="ISB30_003194"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP1990798.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLNIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10695..12542)
                     /gene="mutL"
                     /locus_tag="ISB30_003195"
     CDS             complement(10695..12542)
                     /gene="mutL"
                     /locus_tag="ISB30_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP1990799.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRNMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPSSGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEVPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12552..13883)
                     /gene="amiB"
                     /locus_tag="ISB30_003196"
     CDS             complement(12552..13883)
                     /gene="amiB"
                     /locus_tag="ISB30_003196"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAP1990800.1"
                     /translation="MMYRIRNWLVATLLLLCAQAGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPSPPPMVAKRVETPAVVAPRVSEPARNPFK
                     TESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTI
                     AIARKLRTLLNNDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSA
                     TGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQADPYLSQAVLDLQFGH
                     SQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERL
                     LASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13902..14363)
                     /gene="tsaE"
                     /locus_tag="ISB30_003197"
     CDS             complement(13902..14363)
                     /gene="tsaE"
                     /locus_tag="ISB30_003197"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP1990801.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14335..15882)
                     /gene="nnr"
                     /locus_tag="ISB30_003198"
     CDS             complement(14335..15882)
                     /gene="nnr"
                     /locus_tag="ISB30_003198"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAP1990802.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLR
                     LDRWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMGSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKMSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15881..17020
                     /gene="queG"
                     /locus_tag="ISB30_003199"
     CDS             15881..17020
                     /gene="queG"
                     /locus_tag="ISB30_003199"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP1990803.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGEIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17003..17056)
                     /locus_tag="ISB30_003200"
     CDS             complement(17003..17056)
                     /locus_tag="ISB30_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990804.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3926 a         4921 c         4328 g         4111 t
ORIGIN      
        1 atccaaatct ggatggcttt tcataattca gaggaactag ctgcgctgac gaaccgcttc
       61 aaataagcaa attccggttg caaccgaaac gttcagggaa gaaacacttc ctgccattgg
      121 gatgctgatc aactcatcgc aatgttcacg ggtcaggcga cgcatacctt caccttccgc
      181 gcccatcacc agcgccaggc gtccggtcat tttgctctga tagagtgtat gatccgcctc
      241 gcctgccgta ccgacgatcc agatattctc ttcctgcaac atacgcatgg tgcgcgccag
      301 attagtcacc cgaatcagcg gaacgctttc tgccgcacca caggccactt ttttcgccgt
      361 ggcgttgagc tgtgcggagc gatctttcgg cacaatcacc gcatgaacac cagcggcgtc
      421 cgcgctacgc aggcacgcgc cgaggttgtg cggatcggtt acgccgtcga ggatcagcag
      481 gaacggttga tcgagcgaag cgatcagatc cggcagatcg ttttcctgat actgccgtcc
      541 tggcttcaca cgagcgataa tgccctgatg cacggcaccg tcgcttttct cgtcgagata
      601 ttggcggttt gccaactgga taaccacgcc ctgagactca agggcgtgaa tcagcggtaa
      661 cagacgttta tcttcacggc cttttaaaat aaagacttcc tgaaaacgtt caggggcgcg
      721 ctccagcagg gcctgcactg catggatgcc gtaaatcatt tcgctcatta atgtactcgt
      781 tgtttacgtt ttgccgggag cgccacgctt gcccggccta cggataattc accctcttct
      841 gtttgaagag ggtaatgatc actctgccac ttttttcttc gccgcacgct tcgctttggt
      901 cgctgcggct attttctgcg ttttcgccga tggctttttc gcttttctcg cgtctttctt
      961 cgctgctttc ggcttcggtt ttttctcgcc acggaaggcg ctgtctggct caaagttgac
     1021 ctttttgccg acctgacgcc gcttgccgcc ttttttgcct gcatcgcctt ttttcgcttt
     1081 ctcgcgcgcc gttttaccga cgttgcgcgg tgcgcgttcg ctggagatca ggctaaagtc
     1141 gattttgcgc tcgtccatat taaccgcttc gacgcgaact tccacgcgat cgcccaggcg
     1201 ataagtctga ccgctggatt cacccatcag gcgttgccct acctggtcaa agcgatagta
     1261 gtcattgtcc agcgaagaga catggaccag accatcaatg aacaagtcgt ccagacggac
     1321 gaagaagcca aagccagtga cgctggaaat tacgccttta aagacgttac ctacctggtc
     1381 gagcatgaag tcacacttca gccagtcaga cacatcgcgc gttgcttcat cggcacgacg
     1441 ttccgccatc gaacagtgct gacccagttg cagcatctct tccatcgaat aatggtagcc
     1501 gccggtttca gtggtgttgc cctgatgccc ctgctctttc gccagcagat atttaatggc
     1561 gcggtgcaac gtcaggtcag gataacgacg aatcggcgaa gtaaagtgcg cataggactg
     1621 caatgccaga ccgaagtgac cacggttttc cggatcgtaa atcgcctgtt tcatcgagcg
     1681 caaaagcatg gtttgcagca tttctgcatc aggacgatcg gcaaccgatt ccagtaactc
     1741 tgcatagtcg cgcggttccg gcttgttacc gcccggcaat tccagcccca gctccgccag
     1801 cactgaacgg aaagaggtaa tcgcttcggt gctcggcttg tcgtgaatac ggaacagtgc
     1861 cggttctttg gctttctcaa cgaaacgcgc cgccgagata ttcgccagaa tcatgcactc
     1921 ttcaattaac ttgtgggcgt cgttacgctg agtctgttcg atacgttcaa tacggcgttc
     1981 agcgttgaaa atgaacttcg cttcttcgct ctcaaatgag atcccaccgc gttcttcacg
     2041 ggctttatcc agcactttat agaggttatg taactcttcg agatgcttaa ccagcggcgc
     2101 atactgctcg cgcagatcct gatcgccttg cagtatatgc cagactttgg tgtaggtcag
     2161 acgcgcgtga gaactcatca ccgcttcgta gaatttgtag ccagtcaggc ggcctttcga
     2221 cgaaaccgtc atctcgcaca ccatacacag gcggtctacc tgcgggttga gcgagcacag
     2281 gccgttagag agcacttccg gcagcatcgg gataacctgc gaagggaagt acaccgacgt
     2341 accacggtta cgcgcttctc tgtccagcgg tgttggcgga cggacatagt aactgacgtc
     2401 ggcaattgcg acccacaaac gccagccacc gccgcgtttt ttctcacagt aaactgcatc
     2461 gtcaaagtca cgggcgtctt cgccatcaat ggtgaccagc ggtaaatcgc gcagatcgac
     2521 acggcccgct tttgcttctt ccggcacttc ttctttcagc ccggcaacct gttgctcaac
     2581 agcctgcggc cagatgtacg gaatttcatg ggtacgcaga gcaatatcaa ccgccatgcc
     2641 ggtgcccata ttatcgccca gcacttcgac gattttaccc accgctttgg tacggcgagt
     2701 cgggcgttga gtcagttcaa ccaccaccac aaagcccatc cgcgcgccca tgatctgatc
     2761 gggcgggatt aagatatcga agctcagacg gctatcgtca ggaaccacaa agccgacgcc
     2821 cgcttcggtg aagtagcgac caacaatctg gctggttttt ggcaccagta cgcggacaat
     2881 acgcgcttca cgacgacctt tacggtcagc gcccagcggc tgagccagca cctgatcgcc
     2941 atgaatgcag gttttcatct gctcgctgga gagatacaaa tcatctttac gcccttcaac
     3001 tcgcagaaag ccgtagccat cacggtggcc aataacggta cctttcacca ggtcgaggcg
     3061 ttccggcagc gcatagcact gacggcgagt gaagaccagt tgaccatcgc gctccatcgc
     3121 gcgcaggcgt cgacgcaggc cttcaagctg ctcttcgcct tcaatgtgca gttctaccgc
     3181 cagctcatca cggctggccg gtttttcacg tttggttaaa tgttcgagga taaattcccg
     3241 actagggatg ggattcgcgt atttttcagc ttcgcgttcc tggaaaggat cttgtgacat
     3301 ctcggttcct ccgttgtcat ctctgatgaa gattttcgtc actccaccag caataattta
     3361 taaagcggtt gattctcttc aaccaaatcg gcaagcgtgt agttatccag ttccgtcaga
     3421 aaactttgca cggccttaga aagcgcctgt ttcaaccgac aggcaggtgt aatgtggcaa
     3481 aactcactgc tgcaattcac cagcgataag ggctccagct cgcgcaccac atcaccaata
     3541 cgtatcgcac tcgccggttt acccaagcga atgccgccat tttttccacg aacagcagtc
     3601 acgtagccgg cacgactaag ttgattgatt attttgacca tatgattacg ggagacgccg
     3661 tagacgtcag tcacttcaga aatactggtc atccgccctt ctggcaatga cgccatgtag
     3721 atcagcgcac gtaatccgta atcagtgaaa ctcgttaact gcacatcaac ctcaaaaggg
     3781 aaatcgggga aaaatacatt tatattgatg ataaaccagc cacaagctgt gtcgctaatt
     3841 tatttcagtt cggggaagga aaaaaacgag gatttaacac tgtgtcggat agcgtaatgg
     3901 cttaaccgac acagcaatgt atcacactgt tagcccggca ggcaaaatat ctgccatgcg
     3961 taccagaatt acgcgtcgaa cgggtcgcgc agaatcatgg tttcagtacg atccggaccg
     4021 gtagagatga tatcgatcgg cacaccggtc agctcttcaa tgcgcttgat gtagttcagt
     4081 gccgcctgcg gcaggccact acgatctttc acgccgaagg tggattcaga ccagcccggc
     4141 atggtttcgt aaatcggctc tagacctttc cagtcgtcag ctgccagcgg agtggtagtc
     4201 acttcacgac catccggcat acggtaagcc acgcagagtt taacctcttt caggccatcc
     4261 agaacgtcca gtttagtcag gcagaagcca gacagggagt tcaactgtac cgcacgacga
     4321 acggcaacgg tgtccagcca gccggtacga cgacgacgac ccgtagttgc gccgaattcg
     4381 ttaccctgct tgcagaggaa ctcgccagtt tcatcaaaca gttcggtcgg gaacggacct
     4441 gcccccacac gagtggagta agctttgagg atacccagaa cgtaatcaac ataacgcggg
     4501 cccaggccgg aaccggtcgc cacgccacca gcagtggtgt tggaagaagt tacgtacgga
     4561 tatgtaccgt ggtcgatatc cagcagcgta ccctgcgcac cttcaaacat gacgaaatcg
     4621 ccacgctgac gcgcctggtc gagcaggtca gaaacgtcaa caaccataga agtcaggatg
     4681 tcggcaacag ccatcgtatc atccagaact ttctggtaat caaccgcttc agctttgtag
     4741 tagttaacca actggaagtt gtgatattcc atcacttctt tcagtttttc agcgaaggtt
     4801 tctttgtcga aaaggtcgcc aacacgcaga ccgcgacgtg ctactttatc ttcataagca
     4861 ggcccgatac cacgaccggt ggtgccgatc gctttcgcgc cacgcgcttt ctcacgcgcg
     4921 ttatccagcg caacgtgata atcaaggatc agcggacatg cttcggacag cagcagacgc
     4981 tcacgaacgg ggatgccacg gtcttccagt tctttcatct ctttcatcag cgcagccgga
     5041 gacagcacaa caccgttacc gatgatgctg gttacattct cgcggagaat acctgatgga
     5101 ataagatgga gaacggtttt ttcaccgttg attacgagag tatggcctgc gttgtgaccg
     5161 ccctggtagc gtacaacata tttagcccgt tcagtcagaa gatcgacgat cttaccttta
     5221 ccttcgtcac cccattgggt gcccagtacg acgacgttgt tacccatttt tcaaaatcac
     5281 cgtttgctta aaaatggatt ctaccatcgc tttttcagag ttacagcact ttttgcactc
     5341 aaaaatgacc tcgatcagtc tgttttttgt tcagccaatc gttttcctca acatgtagta
     5401 gaccacaacg cccgcaacca caagtccacc gccaaaacga cgtaaaatat tatcgggcaa
     5461 attggtcatc gcagagatca tcttcttcca tgccttcggg taaagcatcg gccctaaacc
     5521 ttccagtacc aaaaccaggg caagcgccag ccagattgtc gaattcattc attatcctta
     5581 tagaaaaaga aaaccaccga catccctgag gatgcggtgg ctttattgac ctgtaccgca
     5641 gtcgttatat taacgcgttg cggaagtcgg cgtcttcatg tagcggaaga aatcgctgtc
     5701 cgggctcatg accatcacgt cctgattgcc agagaagctg ttctcataag cacgcaggct
     5761 acggatgaat gcgtagaagt ccggatcttt actgaatgca tcagcaaaca gtttggctgc
     5821 ttctgcatcg ccttcaccac gcatgatgcg gccctgacgc tcagcttctg ccagcgttct
     5881 ggtcacttca tagtcggcag tcgcgcgcag tttttccgct tcttcctgac cttgtgaacg
     5941 gtgacgacgt gctaccgctt cacgctcggc gcgcatacgg ttgtagatcg cttcagacac
     6001 ttcggtcggc aggttgatct gcttaattcg cacatcgaca acttcaatac ccagcgccgc
     6061 catactgttc gggttgatga ccggaacttt gcccttcgtc tcagccgtta cgcgctctgc
     6121 cgcttcggca atggcgttat ctgctgcagg ggtagtaact tcatcttctg tacccgcaga
     6181 accggagttc agcgcgtcac gtacttcgag ggtcagacga ccacgggaat cggtgacgat
     6241 atctttcacg tccaggcgac caatttcaga acgcagacgg tcagagaact tacgtttcaa
     6301 cagcacttcc gcttgcgaaa tgtcgccacc acccgttgcc agatagtaac ggctgaaatc
     6361 gctgatgcgc catttgatgt aagagtcgac gatcaggtct ttcttctctt tggtcacaaa
     6421 gcggtcggcc tggttgtcca tggtctgaat acgtgcgtcg agcattttca ccgtttcaat
     6481 gaacggtatc ttgaaatgca gacccggctc ataaaccaga ggtttgttgt catcgtcacg
     6541 cagtacctta ccaaaacgca gcgtaatacc gcgctcacct tctttgacga caaagacaga
     6601 catgtaaagc actaccagca cgatgatgat aatcgcgata actgacttac gcatcgttat
     6661 tccccctgac gctggtagtc gttacgctgc gcgttggcgc ggcgttggtc cataatatcg
     6721 ccctgactgg tggacgacgt gttgcttgct ccactggttg tggaggaaga ggctggcggc
     6781 agacgcagca gattgctggc accgttatcg ctcttcgccg caggggcgtt accacctttc
     6841 agcatctggt ctaatggcag aaccatcagg ttgccacctt tatcgttaac cagcactttg
     6901 cgggtgttgc ccaacacttt ttccatcgtc tcgatataca gacgctcgcg agtaatttcc
     6961 ggcgcggctt tatattccgg cagaagttta gcaaagcgcg ccacttcacc ctgagcttcc
     7021 aggatggtct gggccttgta cgcacgcgcc tcttcgagga tacgttgcgc ctgaccgttc
     7081 gcacgcggct gaacttcgtt ggtatacgct tctgcttcac gaatgtattg ctgttcgttt
     7141 tcacgcgcgg caatcgcatc gtcaaacgcc gcttttactt cttccggcgg acgagcagcc
     7201 tggaagttga cgtccagcag cgtgataccc atgtcatacg gacgaatcgt ctcttccagt
     7261 tcgcgctgag tatcgctacg aatcacggta cgaccttccg tcagaatgcg gtccatggtg
     7321 tatttaccga taaccccgcg tagggcgctg tcggtagcct gacgcaggct gtcatccggg
     7381 ctggtcacgc tatacagata tttttccgga tcggtgacgc ggtactgcac gttcatctca
     7441 acgcgcacta cgttctcgtc cgacgtcagc atcacaccag aagctgccag ttcacgcacg
     7501 gcttccacgt tcaccggttt gacttcgtca ataaacgtcg gtttccagtt cagacccggc
     7561 tcaaccagat ggctgaattt accaaagcgt gttaccacgc cgcgttcggc ttctttaatg
     7621 gtatagaaac cactggccgc ccagataatg acaatcgctg ccgctgcgat ggtaacgacg
     7681 cgaccgccaa gctgcgggcg cgggccttgc gatgaactgc caccgccaga tccggtgcct
     7741 ttaccgccac ccagaccacc gagctttttg ctcagtttgc ggaagatatc atctaaatca
     7801 ggtggccctt gatcgcgacc gcctttgttt ccatttccct cagagttgcc gccaggtttg
     7861 ctgcttcccc acgggtcgcg gtcttgtccg ttattaccgg gctgattcca cgccatgttt
     7921 gtgctccata tttgttatgc ggtgatccct gttgtctttc gacgccagag gatatgactc
     7981 cacgcttcag acgctacgcc gttagatcag gtaatcgatc aacgccggtt cttgtttaca
     8041 gaggcgacgc cagtcaacga tcggcatacg aacttgcaga cttacgctgc cgtcctcctc
     8101 catccactct ttttctattg cctgaagctg ataaaaacga cttctcagac gcccttcctg
     8161 cggcggcaga cgcaatgtat gctgcgccac ctcgccggaa agacgctccg tcaaagcctg
     8221 aaaaagctgt ggtatccccg ctccggtctg cgcagaaagc cagacacgga tcggtttgtt
     8281 ctcttcatct ctgtcaatac gcggttcgaa atcgtccagc atatcgatct tgttcatcac
     8341 cagcagggtt gggatctcgt gagcatcaat ctcttcaaga accgtattca ccgcttcgat
     8401 gttttcttgt acacgcacat ccgccgcgtc aatgacgtgc agcagcaatg tggcttgccg
     8461 cgtctcttgt aacgtggctt taaatgccgc caccagatcg tgcggcaggt ggcgaataaa
     8521 ccctacggta tctgccaaca cggtttcacc gacatccgca acgtcaatac gccgcaacgt
     8581 cgggtcgagg gtggcaaaca actggtctgc cgcgtagacc cgcgcttcgg tgatgcgatt
     8641 gaaaagggta gatttaccgg cgttggtata tcccaccagc gaaacagtag gaacatcggc
     8701 tttgatacgc gattgccgcc cctgctcacg ctgcttttca actctttcca ggcgcgactg
     8761 tatctgcacg atgcgattac gcaacaaacg acggtcggtt tcgagctggg tttcacccgg
     8821 accacgcaaa ccgatcccgc ctttctgtct ttcaaggtgg gtccagccac gcaccaggcg
     8881 cgtagccaga tggcgcaact gcgccagctc aacctgcaac ttaccctcat gggtacgcgc
     8941 acgttgggcg aaaatatcta aaataaggcc ggtgcggtcg atgacacgac actcgcacaa
     9001 acgctccagg ttacgctctt gcgccgggct cagggcatgg tcaaaaagaa cgaccgaagc
     9061 acccgtcgct tttacggctt ccgcaatttc aactgcttta ccttcaccta caaaatactt
     9121 tgggtgcggc gctttacggc taccggtaat cacctgcaat gcttcgacac cggcggaaga
     9181 gaccagagat tcaaactcct ggaggtcttc catatctttg tcttgcgtaa aatagatgtg
     9241 taccagtacc gcctgctcac cagcatcata acggtcaaac aagcgtataa ccctctaaat
     9301 agatcagcgg ggaacgcagg atcgctggct ccccgtgtaa aaaaacagcc cgaaacctta
     9361 ttcggtttct tcgctgtcct gttgcgcgga agtattctgc gcgctgctac catgatgata
     9421 gttactgctg gtaccgccac cggcgttgtt actgtgatga gaaaccgggc gagacgggac
     9481 aacagtagaa atcgcgtgct tgtaaaccat ctggctgacc gtgtttttca acaggatcac
     9541 gaactgatca aaagactcga tttgtccttg cagcttaata ccattcacca aataaataga
     9601 aactggaaca cgttcccgac gcagtgcgtt caggaacgga tcttgtaaag attgcccctt
     9661 agccattctc tcttttcctt atatgcttat ttgtactttg aacctttcga ttctgaaaaa
     9721 attgcgcacg atacgtctca attgtacaca ttcagcctgc gatagcacca acaacctgta
     9781 atacttcgtc acgcgcctgt tctggttttt cactgtcaag ccagtgaacc ccttcccaac
     9841 cacgcagcca ggttatctgc cgcttcgcca actgtctcgt ggcgcaaaca cctcgataaa
     9901 ccatttcatc gtatgagatt tcgccttcaa ggtaagacca catctggcga taacccacgc
     9961 aacgaatgga aggcaaatcc gtatgcaaat ctcctcgggc aaaaagcgcc cggacttctg
    10021 cttcaaaacc tgaagccaac atctgatgaa aacgctgctc aattcgttga tggagcagtt
    10081 cacggctcgc cggggcgatg gcgaactgat gcacctgata cggtagagcg tctcctgacg
    10141 tttgcgtcag ttccgttaaa gttttacccg aaatgaaaaa aacttccagt gcccgggaaa
    10201 gcctttgtgg atcatttgga tgaatccttg ctgccgcaac cggatctacc tcctgaagtt
    10261 gacgatgcaa tgactcccaa ccttgctctg ccgcctgttg ctcaattctg gcccgtactt
    10321 ccgggtccgc cgacggtagc ggcgacaacc cttccagcaa tgccttgaaa tacaacatcg
    10381 taccgcccac taatagtggg atccgccccg ccgcggtgat atcggccatt tccgccagcg
    10441 catcgcggcg aaaatcagca gccgagtaag cctgcgacgg atcgcgaata ttcagcaatc
    10501 ggtgcggcgc ggcgagtaac tcttcagcgt tcggcttcgc cgtcccgata tccatccctt
    10561 tgtaaataag ggcagaatca acgcttatca actctactgg taaaatttta cgcagctcaa
    10621 tggctaacgc tgttttaccg gaggccgtcg gccccatcaa aaaaatcgcc ttaggcaggc
    10681 tcgccttact gatatcactc atctttcagg gcttttatcg ccggatgtaa atcaacagat
    10741 tgtaacagac cacccggcgg cgttttcaca agttgcggac ataaccgttc cacgtccgcc
    10801 agcagggtta tggcctgtgc cattgaccac tgcgcatgtt cgctcatcag atttcgtgca
    10861 atccactgcg caatattgcc aggttcgaat acggactgct tcgccaggta gcctatcagt
    10921 tcaggaatca agatttgtaa attttgttgg cgtaagggta aaggcacggc cctgatggtc
    10981 acatgctgcg catcggactg gaaatcaata cccaattccg ccagggcaga ctgcgctttt
    11041 tctaatgccg atttttcttc gccagaaact tttagccgca acggaataag cagcggttgg
    11101 gcgcaaactg gcacttcacc cggcgtcaat tgtgcctgac gcagccaacg ttctgccact
    11161 ggcaaggata aaagtgaaat gttgccatcg cgctcaagca acgcacagtc ggaatggacg
    11221 atagtcagta cccgaccaaa actctgactg ttcgccgcaa gtgcaggttc ctgcggttct
    11281 ggcgcttttg gtttttgcat cggcgcgggc gtttgcaaaa gctggcgata cacttcacct
    11341 tgctgtttct ggtaacctgg ctgcgcattc ggccaggggg cagccggacg actgcctgag
    11401 gagggcgcag gagtgtaacg cggagctact ggctcacgaa ctgccggttc agcaaaatga
    11461 ttgcgccccg ccgccacgcg attttccgga atggcacgcg gtgcaggttg tggttcatcg
    11521 tccagcggta gcggcgtttc cagttgctgt tgtagcacgc tcagcacgcc ctgatagata
    11581 aagtcatgca ccagacgcga ctgatggaaa cgcacttcgt gtttggcagg gtgcacgttg
    11641 acgtccacct gatgcgggtc gatctccaga tacaacacaa atgccggttg ctgatcggcc
    11701 cccagtttgt cttcgcaggc ctggcggatc gcgtgattga tcaggcgatc gcgcatcatg
    11761 cgaccgttca cgtagcaata ctgaatttct gccagtgcgg gcgtggtgtg atttggatcg
    11821 gccacccagc cgcgcagcgt gagatcgccg tgttgccatt caatcgccag cgcttgttcg
    11881 agaaaagcgg tgccgcaaat cgcgcctaag cgccgttctt tttgcccgcc ttccggcact
    11941 gcgcggtact ggcgtacaat tttgccgtta tgcgacaggt tgatagtgac gtcgaaacgc
    12001 gccagcgcaa tgcggcggat gatttcatcg atatggttaa attcggtttt ctcggtgcgc
    12061 aggaatttgc gccgcgccgg ggtgttgtag aacaggtcca gcacctccag cgtcgttccc
    12121 accggatgcg ccgccggttt aaccgtcacg tccatattgc gcccttcggc ataagcctgc
    12181 caggcttcct gctgttctgc ggtgcgtgaa gtgagcgtca ggcgggaaac cgaactgata
    12241 ctcgccagtg cctcaccgcg aaagcccagg ctgataatgg cttcgagatc gtccagagag
    12301 gcgattttac tggtggcatg acgcgccagc gccagtgcca gctcatcttt tttgataccg
    12361 cagccgttat cacgaatgcg gataagtttc gccccaccgc gttcgatatc aatatcgata
    12421 cgcgtcgcac ctgcatcgag gctgttttcc accagttctt tgactaccga cgcaggtcgc
    12481 tcgaccacct cacctgcggc aatctggttc gccagttgtg gcggtaagac ctgaattggc
    12541 atcaatcgtc cttagtttgg cagcgtgcga tctggcgtcg tcaccgtact ggccgtttgt
    12601 gccgttgcac cctgcggcgc agattgcatc ggatgcgcaa ggaagtaatt gcgcaggcct
    12661 ttgtaaatgg cttctgccag ctgttgttgg taatcgtcgc tcgccagcaa acgttcttcg
    12721 ctgttgttac tgataaaacc ggtttcgacc agtactgacg gaatatccgg cgaacgcaga
    12781 acgccgagac tcgcgtgttc tggtcggcgt ttatgtattt cgccaatgcg ttgcaactga
    12841 ctgatcatac tggtcgctac atcataccct acccgctggg aatgaccgaa ctgtaaatcc
    12901 agcaccgcct ggcttaaata ggggtcggcc tgactgttcg ccagcacatc acccgccccg
    12961 cccagcagct ccgactgctt ctcatgctgt tccagccagc tggccatttc gctgttggcg
    13021 cgacggtttg agagcaccca tacagaggca ccggtggcac tacggtttgg tgcggcatcg
    13081 gcatgaatcg acacaaggaa attggcgttt tgcttacgtg ccacatcact gcgccccatc
    13141 accgagataa agtaatcccc gtcacgggtt aaaacgccct taaacatcgg atcgttattg
    13201 agcaaggtac gtaatttacg cgcaatggcg atggtgacat ttttctcccg tgtaccacca
    13261 gggccgatag cgccagggtc ctgaccgccg tgtccggcat caatagcgat gataatttta
    13321 tcgcctgtgt tagccgtcgc gcgcgctgcc ggacgcgtta ccgtattact gctgataaca
    13381 cccgtagtgc ggttactttc cgttttaaac ggattgcgcg ccggttcgct gacgcgcggt
    13441 gcgacaaccg caggcgtttc aacgcgtttc gcaaccattg gcggaggcga tggcggcgca
    13501 tcggcgttaa tcgtaaagac aacagtgtaa ttgctgccat tctgccgctt caccgcttcg
    13561 gttttaccgt tttcggtgag atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt
    13621 ccggagcgaa tcgctttcac caggttatta ccgctaaaca acaacggcag cccctgaatt
    13681 acccccgttt gtttgatatc gagcgccacg gtgcgtttgc tttgatggct aaacgcataa
    13741 tcaggatcgc caataaaact caacgttatc cgcgcctgtt gattaccgtt agaaacctga
    13801 atatcagaga gcgtcgcggc ccccgcctgc gcgcacagca gcagcaacgt cgctactaac
    13861 caatttctga tgcgatacat catcccgcca cctttcaaag gttaaccggc taaacgcgcc
    13921 agcaacaatt cacccgcaga ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga
    13981 taatcaatgt gtatttcgac atccgggtca ggaagaacac ctgtaccttg ttgtggccac
    14041 tccaccaggc agatggcatc gttggcaaaa taatcgcgga tccccataaa ctccagctcc
    14101 tcgggatcgg caaggcggta caaatcaaag tgataaacca ttaaattgtc gagcgtatag
    14161 ggttcgacca gcgtataagt ggggcttttg acattaccct gatgacccag agcctgtaaa
    14221 aagccccggc taaaggtggt tttacctgcg cctaaatcgc catacagata gattacggtt
    14281 gcgccatcgc aggctttcgc tacccgctcg cccaggtcta atgttgcctg ctcatcaggg
    14341 agcggaatta ctcgattcat catggttttt atcagtcact tccgggttaa caatacgctg
    14401 tagcgtggaa aagagatcgg ttgccagcat cccgcgcgtt ccaaaacgcg ccgccagtac
    14461 gtcagctgcc gcaccgtgcg cgacacagcc cgcacaggct gcatcataag ggctcatttt
    14521 ttgcccaagc aatgcgccaa taataccaga gagcacatcg cccatgccgc cgctcgccat
    14581 gcctgcattt ccggcatcaa taatgcctaa agcgtcagga tgggcggcga ccacggttcc
    14641 ggcacctttc agcaccgcta cgccgccata acgttgtacc agacgttggg cgcaatgtaa
    14701 gcggtcactt tcaatttcag cgacggaaca tcctaacaac cgtgcggcct caccaggatg
    14761 cggcgtgatc acgcgattgt gacgcttatc gggattgatt gccagcaggt tcaatgcatc
    14821 ggcatcccac aacatcggtt tgcgaaaatt ctcaactttt tgcagggctt ttttccccca
    14881 ctcttgctgg cccagaccgg gaccaatcac caccacatcg gcccattcca ggctttcggt
    14941 aagagagccc atagtcagtt catgcaccat caattccggt cgtgcagtca gcagcggcgc
    15001 aatgttctca ctgcgggtca gtactcggac taaaccagca ccagcacgca gcgccgcttc
    15061 ccccgtcata cgaatagccc ccgccgtgcc gtgatcgcca ccaataatca ccagccgccc
    15121 gtgatcgcct ttatgcgaag tcgggcgacg cggtatcagc cactgagaaa gttgttctgc
    15181 cgaaaaccgc tgaattttcg tctcctgacc tgccagccat ctatccagcc gcagtgagtc
    15241 aaaatgcagt tgtccggtaa catcccgcgc ttttccagtg agcaaacctg gtttcagcgc
    15301 aataaaagtg atggtgtgat cggcgttgat taccgcgcct ggcgtagcgc cggtttcagc
    15361 cagaaggccg gaagggatat caaccgccac aatcggcgca ggatgggaat tagcgtggtc
    15421 gattaactgg ctaatggatt cgcggggcgc ttgctgcaaa ccggtgccga gcagcgcatc
    15481 gacaatcaga tctaccgatt cgggccagac aatattcgaa gcatggatct cgcctcccgc
    15541 gtttaaccat gcttcgcgtg ccagcgcggc ctcttccggc aacggtttgt cactttcctg
    15601 ggccagcagc gtgacatcaa taccgatcgc tttggccagc cgcgcgacca cgtagccatc
    15661 gccgccgtta ttaccatgac cgcacagcac cagccagtgg cgggcgtcag gataagccga
    15721 acgacacacc tggaatgcgg cttcgccagc gcgaagcatc agctcataga gtgtgagccc
    15781 cagcgcatct gccgcctcgc gttctccgcg gcggatatcg tcggcgtacc agacggtgtg
    15841 tggtatactt acggggtttt tcttcattgt atggtccgtc atgtcagagc ccctcgatct
    15901 caatcagtta gcgcaaaaaa ttaaacagtg ggggctggaa ctgggctttc agcaggtagg
    15961 tattaccgat accgatctca gcgagtccga gcccaaactg caagcatggc tggacaaaca
    16021 ataccacggc gaaatggact ggatggcgcg tcacggtatg ctgcgtgctc gtcctcacga
    16081 attattgccc ggtacgctgc gcgtgatcag cgtgcggatg aattaccttc ctgctaacgc
    16141 cgcatttgcc agcacgctga aaaatcccaa actcggctat gttagccgtt atgcgctggg
    16201 ccgtgactat cacaagcttc tgcgcaaccg actcaaaaag ctgggcgaga tgattcagca
    16261 acattgtgtt tcgctgaatt ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc
    16321 gttagctgaa aaagcaggtc ttggctggac aggtaagcac tcacttatcc tcaatcgcga
    16381 ggccggttcg ttcttctttt taggcgaatt gctggtcgat attccgctgc ccgtggatca
    16441 accagtcgag gaaggatgcg gtaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc
    16501 catcgtcgag ccatataccg tcgatgctcg ccgctgtatc tcttatctca ccatcgaact
    16561 ggaaggggag atcccggaag agttgcgacc gttaatggga aaccgtattt acggttgcga
    16621 tgactgccag cttatctgcc cgtggaatcg ctattcgcaa ctcactacag aagacgattt
    16681 cagcccgcgt aagccgctac acgcaccgga actcattgag ttattcgcct ggagtgaaga
    16741 gaagttttta aaagtcacgg aaggttcggc gattcgccgt ataggtcacc tgcgttggct
    16801 gcgtaatatc gccgtagcat taggcaatgc cccctgggat gaaacgattt tgacagcgct
    16861 ggaaagtcgt aaaggtgagc acccacttct tgatgagcac atagcgtggg cgattgcgca
    16921 gcaaatcgag agacgaaatg cgtgcgtggt cgaagtgcaa ttaccgaaaa aacagcgtct
    16981 ggttcgggtg attgaaaaag ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat
    17041 aaaaataaaa acgcattgca attcaagccg gaaaaaatcg tcaagtgatc gatacgacaa
    17101 aacgaaatct taatttttaa cttatctatc aataggttaa aaaaatacta ttcaccgtgc
    17161 gaagtttctt tgaaattaag attacggcct gaggctgtgg ataactctgt ttacaagagt
    17221 ttgtcagaga caacaaaaac cagcaataac gcatcgcgtc gctgtggata ttttattgag
    17281 agaaga
//
LOCUS       DACADF010000100        17271 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.102, whole genome shotgun sequence.
ACCESSION   DACADF010000100 DACADF010000000
VERSION     DACADF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.102"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(193..1386)
                     /locus_tag="ISB30_003201"
     CDS             complement(193..1386)
                     /locus_tag="ISB30_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP1990805.1"
                     /translation="MKKIVLPVALALLGMNAGAYAADVSIDILSATVKDKRIEGASVT
                     LQRNGAQSVSGITNTSGSISLGTSFADNKDALLIVKKEGYSNLVVKCPCAGMTYAISP
                     VMTNLDGMRVVLSWGEKPFDLDSHLIFPGGHIYFDSKEGTDANLDVDDTDSYGPETVT
                     ISKKHFGESYIYAVQDYSNKGLPNSNYLSASKAKVFVYVGSSLVRSYSVPAGKRGNIW
                     TVFKLNPNGEFEDINSVTSANFNGTTLDVRDLATVIMPATDSSAPASPAMQNSGDTQL
                     ARKYNREGEAVYKTGQLEQAIQLFQQATELDGNYGQAFSNLGLAYQKNGNIAEAIWAN
                     RKAISLASGVNAATTRANSYYNIAKIYETSGQNAEALQHYQLAYTEKNKPSYEEAIAR
                     VKTKM"
     gene            complement(1584..4217)
                     /gene="mngB"
                     /locus_tag="ISB30_003202"
     CDS             complement(1584..4217)
                     /gene="mngB"
                     /locus_tag="ISB30_003202"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAP1990806.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEGGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLAILKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFDYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRGSQFNLRDGRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQISQIVPSMGYRTLYIEANQLGNVVTPKSKTEGILENAFWQIALNEDGSLRLV
                     DKDSGVRYDRVFQIEEGSDDGDEYDYSPAKEEWAITSANAKPQYDIIHEAWQSRAIIR
                     YEIAVPRNLSERRAKQCSGRVGVETVITLSHNSRRIDADINLDNQADDHRIRVLIPTP
                     FNTDVVLADTQFGSLTRPVKDCAMNVWQQEGWKEAPVPVWNMLNYAVLQEGRNGIAVF
                     SEGLREFEVIGEENKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVSDSQLRGSFSC
                     RLSLFSYIGTPVTAGVAQQARAWLTPVQCYNKIPWDAMKLNKAKFNVPESYSLLKMPP
                     VGCLISALKKAEDRQELILRLFNPAELTSCDATVAFSRKVMTCTETMMNERFNTEKNE
                     VLKELALFLPGQSRTFSYRIV"
     gene            complement(4235..6151)
                     /gene="mngA"
                     /locus_tag="ISB30_003203"
     CDS             complement(4235..6151)
                     /gene="mngA"
                     /locus_tag="ISB30_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAP1990807.1"
                     /translation="MNLTTLTHRDALCLNVRFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYCRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMKLLTALTTRLADDELRARIQSATTPDELLSALDDKGDAQHTASF
                     SYAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNIYVEKQGANGIEERLTAEQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGMLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGNNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRHH
                     AVKHGNYLTDGVMP"
     gene            6320..7042
                     /gene="mngR"
                     /locus_tag="ISB30_003204"
     CDS             6320..7042
                     /gene="mngR"
                     /locus_tag="ISB30_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAP1990808.1"
                     /translation="MGHKPLYRQIADRIREQITCGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVETHSEVLIF
                     EVIPADNFLQQQLQISEQDRVWHVKRVRYRKQKPVALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGQVFEY
                     SRNAFNTDDYKFTLIAQRKSPR"
     gene            complement(7146..8015)
                     /gene="sucD"
                     /locus_tag="ISB30_003205"
     CDS             complement(7146..8015)
                     /gene="sucD"
                     /locus_tag="ISB30_003205"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAP1990809.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(8015..9181)
                     /gene="sucC"
                     /locus_tag="ISB30_003206"
     CDS             complement(8015..9181)
                     /gene="sucC"
                     /locus_tag="ISB30_003206"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAP1990810.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(9275..10492)
                     /gene="odhB"
                     /locus_tag="ISB30_003207"
     CDS             complement(9275..10492)
                     /gene="odhB"
                     /locus_tag="ISB30_003207"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAP1990811.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDVVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(10507..13308)
                     /gene="sucA"
                     /locus_tag="ISB30_003208"
     CDS             complement(10507..13308)
                     /gene="sucA"
                     /locus_tag="ISB30_003208"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAP1990812.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAISEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(13670..14386)
                     /locus_tag="ISB30_003209"
     CDS             complement(13670..14386)
                     /locus_tag="ISB30_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAP1990813.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(14402..16168)
                     /gene="sdhA"
                     /locus_tag="ISB30_003210"
     CDS             complement(14402..16168)
                     /gene="sdhA"
                     /locus_tag="ISB30_003210"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAP1990814.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(16168..16515)
                     /gene="sdhD"
                     /locus_tag="ISB30_003211"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(16168..16515)
                     /gene="sdhD"
                     /locus_tag="ISB30_003211"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAP1990815.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(16509..16913)
                     /gene="sdhC"
                     /locus_tag="ISB30_003212"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(16509..16913)
                     /gene="sdhC"
                     /locus_tag="ISB30_003212"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAP1990816.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
BASE COUNT         4150 a         4681 c         4175 g         4265 t
ORIGIN      
        1 ccagccaatt ataaagatct tgctcctcaa aaaatgaaac aactcagcct gagataatat
       61 ttttgaccga agttaacatt aaaaatggtt aatagtttgc gcctctcaca caggtccagg
      121 caactgcgta acaggcaaat caaattttcg taaacagtgg gcatcatcag atgcccacaa
      181 aatacagctg atttacattt tcgtctttac acgtgcaata gcttcttcat aagaaggctt
      241 atttttttcc gtatacgcca gttgataatg ctgcaatgct tcagcattct ggcccgaggt
      301 ttcataaatt ttggcaatgt tataatagga gttggcgcgc gttgtcgccg catttacccc
      361 actcgctaac gagatcgctt tacgatttgc ccaaattgct tccgctatgt tgccattttt
      421 ctgatacgcc agacccaggt tactaaatgc ctgtccgtaa ttgccgtcta attctgttgc
      481 ctgttggaag agttgaatgg cttgctcaag ttgaccggtt ttataaacag cttcaccttc
      541 gcgattatat tttcttgcca gttgagtatc tccactgttt tgcatcgcag gcgaagccgg
      601 agctgaagag tccgtcgcag gcattatcac agtggctaaa tccctgacgt ctaatgtggt
      661 gccgttaaaa ttggcgctgg taacagagtt gatatcttca aactcgccgt ttggattgag
      721 tttaaacacg gtccagatgt tccctcgttt tcctgcaggg acactataac tacgtaccag
      781 ggagctccct acataaacaa acacttttgc cttactggca gagagataat tactgttggg
      841 cagcccttta ttgctatagt cctgcacggc atagatgtaa ctttcaccaa aatgcttttt
      901 gctgatagtg acagtttctg gtccatagct atccgtatca tcaacatcca gattggcatc
      961 agttccctct ttactatcaa aatagatatg tccgccaggg aagatcaaat gcgaatcaag
     1021 atcaaaaggc ttttcgcccc agctcaaaac tacacgcatg ccatccagat tcgtcatcac
     1081 cggactgata gcataagtca tgccagcgca cggacatttg accaccagat tggaataccc
     1141 ctcttttttg acgattaata gagcatcttt attatcagca aatgaagtcc ccaaactaat
     1201 gctgcccgaa gtatttgtga taccagaaac agattgtgca ccattgcgtt gcagcgtcac
     1261 cgatgcccct tcaatgcgtt tatctttcac tgtcgcactt aaaatatcaa tgcttacatc
     1321 ggcggcataa gcacctgcat tcatgcccaa cagcgcgaga gcaactggca aaacaatttt
     1381 cttcatgtat aaatcctttt tttaatgaat gtaaggtgga aatttcccag ttattattat
     1441 cgtgttaaaa ggtgaaaaaa aagaaataag tacacttttc agattaacac ccactcacct
     1501 tgaaacaaaa aggccatccg gagatgacct gaactttctg agaaacgctg ttttttcatg
     1561 tggtttgctt tattacacac cattcagact attcggtagc taaatgtccg cgactgtccc
     1621 ggtaaaaaga gagctaattc ttttaaaacc tcattttttt ctgtattaaa gcgttcattc
     1681 atcatggttt ctgtacacgt catcaccttc ctgctaaacg cgacggtcgc atcacagctt
     1741 gtcaattcag ccggattaaa cagccgcaat attaattcct gtctgtcttc tgcttttttt
     1801 agcgcgctga tcagacatcc cactggtggc attttcagca aactatagct ttccggcaca
     1861 ttgaatttgg ctttattaag cttcatcgca tcccacggga ttttgttata acactgaact
     1921 ggcgtcagcc atgcgcgtgc ctgctgggct acaccggcag ttacgggagt accgatataa
     1981 ctaaataaac tcagacgaca ggaaaatgat ccgcgtaatt gtgaatcgga cacaggcatt
     2041 ttaattcctg aaggacgccc tggccttaaa agtaaatctt cttttcccag taatcccaca
     2101 ccacgcaaca acgtaatagc aaaggtttta ttctcttctc caatgacttc aaattcacgt
     2161 aatccttcgc taaaaacggc tatgccgtta cgcccttcct gtaagacagc atagttgagc
     2221 atattccata ccggaaccgg cgcttctttc cagccttctt gctgccagac attcattgcg
     2281 caatccttca ctggccgcgt caatgaacca aactgcgtat ccgcaagaac aacatctgtg
     2341 ttaaaaggcg tgggaatcaa tacacgaata cggtgatcat cggcctggtt atcaagatta
     2401 atatccgcat caatgcgtct gctgttatgg ctaagagtga tcaccgtctc cacccctacc
     2461 ctgccagaac attgctttgc gcgacgctct gacagattac gcggcaccgc tatttcatag
     2521 cgaattatgg ccctactctg ccaggcttca tgaataatat cgtattgcgg tttcgcattc
     2581 gctgaggtaa tggcccactc ttcttttgct ggcgaataat catattcatc cccatcatcg
     2641 gaaccttctt caatttgaaa cacccggtca tagcgcacgc cactatcttt atcaaccaga
     2701 cgaagtgaac catcctcatt gagtgcaatt tgccagaaag cattttccag tattccctct
     2761 gttttacttt ttggcgtgac cacattgcca agctgattcg cttctatata gagcgtgcga
     2821 taccccatag aagggacaat ttggctgatc tgaatatcaa actccataaa gggatcataa
     2881 ttgccgtaat gaactatttg ccgatcaata aggccgggat cgatctcacg ggcatgtcga
     2941 ataaaatatg gcacaggctg accgcgaccg tctcgcaaat taaactggct accgcgcagt
     3001 cgaacagttg tgttgataac ttcttcacgc ggccagggca tcaggttaaa caggacgagt
     3061 ttgtcggcgt cgctctgcgg catgttgtct gctattttgc gcatatagaa ccgaattaga
     3121 ttatccgcca tgtcttctgc cagttcgaag cgggcgacaa tctcgcgatg aactttatca
     3181 ctgcagcagc agccgatgct gtcatgggcg tggtttttta aaatttcttt ccacatcttc
     3241 tccagcaagc cgtggtgata atcaaaacct aaagtccagg ccagcgttgc caacggttcc
     3301 agcagattaa caatcttatt ttcaatgcgt gcgtgggcaa ttttgatatc catgcgcgtg
     3361 gaaccgatgg tgcgatgcac gcgcatatat ttgccatcga tgaattcacc tttcaggatg
     3421 gccagacttt cacgctgcgc ttcaatctgc tcgaatacct cttcaaagcg gctcatcaca
     3481 aactttcgtt gaggataaat ttcgcgcagt ttctccatca cttcgaagat attttgctgc
     3541 aagggcatct ggtcatgacc attcggcaat aaaatttctt tggttactga cgctttttcc
     3601 agcacgtcaa aataactgtc gaggcgttta cgtaatccat tttcgtccgc aggtaagtac
     3661 ttaccgatgg cgtagcccag cggcagcacc tgcgccgtca cttcgctacc gtcactgctt
     3721 tgccacaaaa actcggtttt atcagtaccg tggcgctcag aacatccgcg ccagaacatg
     3781 gtgcgggtaa tgccaaatcc attgtatata tgtggcagtt gcccggacat gccaaaggaa
     3841 tccggtaaat aacctatttt catcggctcg ccaaacgcga ggcagtcacg cattccgtac
     3901 atcagattac ggactatgga ttcagcagaa acaatcgtgg tatcggtctg ggtataccag
     3961 ggaccgataa ttaatttccc gccctcgacc aacttcttca cacgatcttt gttctccggt
     4021 ttgacagcga aataatcttc gaggatcgcc gtttgcccgt cgagtacgta atatttgtat
     4081 tcgttgtcct gttccagtct gcacaggatc tcttccatat tattgaccag cagaatgcga
     4141 gactcttctg tggtgaaata ccactcccga tcccagtgca tatgcggggt gatgtgaacg
     4201 cgagataccg ctttcatcgt cgtttccttt ttgtttatgg cattacgcca tcagtcagat
     4261 agtttccatg ctttaccgca tgatgccgcc agatcaggag aattgcagtc gagattgcag
     4321 cccccaccaa cgccgcacca aaccaaccaa ttgccgccat aacaccaccc gcgccattat
     4381 catgaagtaa aaagagcgag aaaatgccgg caccgggtgt cgaaaggccg atattcatcg
     4441 caccgacgat agcgcccgtt accatagatc ccagcacaaa cgaaccaata acccgcagcg
     4501 gatcttcaat cgccatcggg atcgcccctt cggtgatacc tgccaatcct aacagccagg
     4561 tggatttccc ggtttcaatt tcaaactctt taaacaggcg cggtgcgagc atcgtggaag
     4621 cggttacggt aaatgccgaa accattttga cggaggcgaa aatggcatac gggccgtaaa
     4681 cgccgttcgc cattgccccc agacagaatg cataagcggc tttgttcact ggcccaccaa
     4741 ggtcaaagga acacataaaa ccgagaatgg cgcccaacaa cagtgcgtta tttcctgaca
     4801 gaccgttcag ccaggcggta agcgagttat tgatccatgc gacaggttcc cctactacaa
     4861 acagcatcag actacccgcc cccaacgtac cgagcaccgg gtagagatag aaagtcagga
     4921 atccgttgaa tttactgcta agacgtagat gatttttcac ccagcgcatc agataaccgg
     4981 ctatcaatcc gccaacgacc gcgccgagaa acccggaacc aatcatgttg gcggcaagcc
     5041 ctgccgcaaa gcctggcgct aacgccggtt tatcagccag tgaataggcg gtataggctg
     5101 cgagcaccgg taccatcaaa atcccgagca tcccgccgcc cagcttgcgg tacatccaca
     5161 accaggagtt ttcttcatta aacaaatctt gtagcccgaa gatttgcgac agtaataccg
     5221 cgaccgccag caccgtgcca cccgcgacaa tcagcggcac ggcgaaagag atcccgctca
     5281 acagtgcctg cttcagctcc gttttgacac ttttcaccgg ttgcgtatct tgctgtacgg
     5341 tacgcgtctc atcgctacgc ttgagggtaa gcgcttgttg aatcaacgct tctgcatggc
     5401 gaatcggctc ggcaacaggc acagaaagcg cgggaatgcc gttaaaacgc tcgctctcct
     5461 tgatagcgac ttccgccgca aaaatacagg cggttgcact attgagttgc tccgccgtta
     5521 aacgctcttc aatgccgtta gcaccttgct tttcaacgta gatattcacg ccgagtttgc
     5581 gtccggcttt ttccagatat tccgcagcca tgtaggtgtg agcaataccc gccgggcagg
     5641 ccgttacgca gacgatagtt ggcgcataag aaaaagaggc agtatgttgc gcgtccccct
     5701 tgtcatccag cgccgagagc aattcatcgg gcgtcgttgc cgactgaata cgcgccctga
     5761 gctcatcatc cgccaggcgc gtggtcagcg cggtaagcag tttcatatgc gtagtacctg
     5821 cttcattggg aggaatggcc agcaatacca ctaaatcaac cgcttccggg ccatccacgc
     5881 cttcccactg aagcggctcg ctgagtgtgg cgaccgcaaa cgccgcttct tttaccgcag
     5941 cagttttgcc atgcggcacg gccagccctt cacctaaggc ggtcgggccg aggctttcac
     6001 ggcaatacac ttcttccaga aattgctcag tactggaaat tttccccaga gcagcaagac
     6061 gttgagtcaa cgcgtggatg gcctcttcac ggctggtaaa gcgcacattc agacacagcg
     6121 catcgcggtg ggtcagagtc gtcaggttca tcatggttct cacttgatca tttttatagt
     6181 cgcgcaagtg tgcctgataa aacaatacat ttacgtgatc actttcactt acaagatact
     6241 atttgtatta aatttgtatt atttacaaga tgaatttatc gagcataatg gctaaattcg
     6301 tattaatgag atactagaaa tgggacacaa acccttatac cggcagattg ccgatcgcat
     6361 tcgtgagcag attacgtgcg gcgagctaaa acctggcgat gcgttaccca ctgaatcggc
     6421 attacaaaca gagtttggcg tcagccgggt tacggtgcgc caggctttgc gccagttagt
     6481 cgagcagcag atcctcgaaa gcatccaggg cagcgggact tacgtcaaag aagagcgggt
     6541 caattacgat atttttcagt taaccagttt tgatgaaaaa ctgtcggacc gtcacgtcga
     6601 aacacatagt gaagtcctga tatttgaagt aattccggct gacaattttc ttcagcagca
     6661 gctgcaaatt agcgagcagg atcgtgtgtg gcatgtgaag cgcgtgcgct atcgcaagca
     6721 aaagccggtg gcgctggaag aaacctggat gccgcttgca ttattcccgg atctcacctg
     6781 gcaggtcatg gaaaattcga aatatcactt tattgaagaa gtgaagaaga tggttatcga
     6841 tcgtagcgaa caggagataa ttcccctgat gcccacggaa gagatgagcc gtctgcttaa
     6901 tatcagccag actaagccga ttcttgaaaa agtatcgcgt ggatatttag ttgatggcca
     6961 ggtgtttgaa tatagccgca acgcttttaa tactgatgac tataaattta ccctgatagc
     7021 gcagcgaaaa tcgccgcgat aagcataaaa aaggccatca taagatggcc aaccatgtcg
     7081 aaaccgacag cccttcgccc ttcctgtagg gggaagcggc gagggctatt tcttattaca
     7141 gatatttatt tcagaacagt tttcagtgct tcaccgatat cagccaggct gcgaacggtt
     7201 ttcacgcctg cggcttccag agcagcgaat ttctcatccg cagtaccttt accaccggca
     7261 atgatggcac ccgcgtggcc catacgtttg cctttcggcg cagtcacacc agcgatgtaa
     7321 cccacaactg gcttggtgac gtgctctttg atgtacgcag ccgcttcttc ttcagcgcta
     7381 ccgccgatct caccgatcat cacgatcgct tcagtctgcg gatctttttc gaacatttcg
     7441 agaatatcga taaagttaga gccagggatc gggtcgccgc cgataccaac acaggtcgac
     7501 tgaccgaaac cgtaatccgt ggtctgttta accgcttcat aggtcagcgt accggaacgg
     7561 gaaacgatac ccactttacc tggtttgtga atgtgacccg gctggatacc gattttgcat
     7621 tcccccgggg tgataacgcc tgggcagttc gggccgatca tacgaacgcc tgcttcatcc
     7681 agtttcactt tcaccgtcag catatccagc gtcgggatgc cttcagtgat ggtgataatc
     7741 agtttgatgc ctgcatcgat agcttccaga atggagtctt tgcagaacgg tgctggtacg
     7801 tagataacag aggcggtagc gccagtggca gcaacagctt cacgcacggt gttaaacacc
     7861 ggcaggccga ggtgggtagt accgccttta cctggggtta cgccgccaac cattttagtg
     7921 ccgtatgcaa tggcctgttc agagtggaaa gtcccctggc taccagtaaa gccctggcag
     7981 ataaccttgg tgtttttatc aattaaaatg gacattattt cccctccact gcggcaacaa
     8041 cctgctgagc tgcatccgtc agaccttttg ctgcaataat attcaggccg ctgtcagcca
     8101 gtttcttcgc accgagttcg gcgttgttac cttccagacg taccacgacc ggtacgttaa
     8161 cacccacttc tgctaccgcg ccgatgatac cgtcagcgat caggtcgcaa cgaacgatac
     8221 cgccgaagat gttaaccaga acggctttca ctttgtcgtc agagaggatg attttgaacg
     8281 cttcggttac acgttctttg gttgcgccac cgccaacgtc caggaagtta gccggttcgc
     8341 cgccgtgcag tttaacgatg tccatcgtac ccatcgccag acctgcgccg ttaaccatac
     8401 aaccgatgtt accgtccagc gcaacgtagt tcagttccca ctgtgcagcc tgtgcttcac
     8461 gcggatcttc ctgcgactgg tcacgcattt cacgcagatc aggctggcgg aacagtgcgt
     8521 tgccatcagc gcccagtttg ccgtcgaggc aaatcagatc gccctgtttg gtaataacca
     8581 gcgggttgat ttcgataagc gcaaggtcgc gctccaggaa aatggtcgcc aggcccatga
     8641 agattttggt gaactgctga accagtttac cttccagacc cagtttaaac gccagctcgc
     8701 gtccctgata cggcatcggg ccagtcagcg gatcaagcgc aactttgtgg atcaggtgcg
     8761 gagtttcttc cgccactttt tcgatttcca cgccgccttc ggtggaggcc ataaagacca
     8821 cgcgacggga actacggtca acaacggcac cgagatacag ctctttagcg atatcggtcg
     8881 ctgcttcaac cagaatctgg ttaaccggtt ggccattggc atctgtttga tacgttacca
     8941 gacgcttgcc cagccagttt tctgcaaaag cacggatgtc ttctttgctg tttacaactt
     9001 tcacaccgcc cgctttaccg cggccaccag cgtgaacctg acatttcact acccacggac
     9061 cggcaccgat ttttgaagcg gcttcttctg cttcgcgtgg agtagtacag gcataaccca
     9121 ccggtgctgg taagccatag cgggcaaaaa gttgttttgc ctgatattca tgtaagttca
     9181 tgtgttctgt ccatccttca gtaatcgtta tcttttaaac cgtagaccga ccccctatcc
     9241 acgtcggtct acagtgcagg tgaagcttaa actactacac gtccagcagc agacgcgtcg
     9301 gatcttccag caactctttg atcgttacca ggaagcccac ggattcgcga ccatcgatca
     9361 aacggtgatc gtaggacagc gccaggtaca tcatcggcag gatctcaacc tggccattca
     9421 ccgccatcgg acgatctttg atagcgtgca tacccagaat tgcgctctgc ggcgggttga
     9481 tgataggcgt agacatcagg gaaccgaaca caccaccgtt ggtgatggta aagttaccac
     9541 cagtcagatc ttcaaccgtc agcttgccgt cacggccttt aactgccagc tctttaattt
     9601 tcttctcgat gtctgccatg ccgagggtat cgacatcacg cagaaccggc gtcaccaggc
     9661 cgcgcggcgt agaaaccgcc atactgacgt cgaaatagtt gtggtagacc acatcatcgc
     9721 cgtcgataga cgcattcact tccgggtaac gtttcagggc ttcaaccacc gctttcacgt
     9781 agaaggacat aaagcccaga cggatgccgt ggcgtttttc aaacgcttca ccgtactgct
     9841 tacgcagatc cataatcggc ttcatgttga cttcgttgaa cgtggtcagc atggcggtgg
     9901 agtttttcgc ttccagcaga cgctctgcca cacgcttacg caggcgagtc atcggcacac
     9961 gtttttcgct acgtgcagcc agggccggtt gcgccgccgg agcagccgct gccggagcag
    10021 actctttcgc cggggctttc gccagatgtt tttccacatc ttcacgggtc agacgaccac
    10081 ccacacccgt gcctttaatg gcgctggcgt cgaggttatg ttcagccagc aggcgacgga
    10141 tcgccgggct taacgcatcg ttgttttgct cttccagaga cgcctgctgg cgttgcgccg
    10201 gagtggacgc tttctcttca gatttggcgc tggtttcttt accggcgctg ttgccttcac
    10261 gcaggcgacc aaggatctga cgagacgtta cggttgtacc ttcatcttcc agaaccgcat
    10321 ccagaatgcc gtctgctgat gccggtactt ccagtaccac tttgtcagtt tcgatttcta
    10381 ccagcacttc atcacgtacg actacgtcgc cgggtttttt atgccaggtt gcgacggtgg
    10441 catcggctac ggattcaggc aggtcaggga ccagaatatc tacgctactc attgtgtatc
    10501 ctttatttat tcgacgttca gcgcgtcatt aaccagatct tgttgctgtt tctggtgaac
    10561 ggacatatac cctaccgccg gagaggcgga ggccgggcgg cctgcataac gcagagaagc
    10621 cccaaacgga atcacttcac ggaaatgatg ctggctgcag taccatgcgc cctggttgag
    10681 cggctcttcc tggcaccaga caaaatcctt gacgtgagca aactgctgca acacttcctg
    10741 catcgcttta tgcgggaacg ggtagagttg ctcgatacgc acaatggcga catcgtgttg
    10801 attgttctta cgacgttgtt ccagcagatc gtaataaacc ttaccagaac acatcactac
    10861 gcgcttcacg cccttcggat caagctcgtc gatttcactg atggctggca ggaaggtgcc
    10921 gttcgccagt tcttcgaggc tggaaaccgc cagcggatga cgcagcaggg atttcggcga
    10981 catcacgacc agtggacgac gcatcccgcg caacgcctga cgacgcagca tgtggtaaac
    11041 ctgtgccggg gtagaaggta cgcaaacctg catgttttgc tcagcgcaaa gttgcagata
    11101 acgttccaga cgcgcggagg agtgctccgg cccctgccct tcgtaaccgt gcggcagcaa
    11161 catcaccagg ccacacatcc ggccccattt ctgttcgcca gaggagatga actggtcgat
    11221 aaccacctgt gcaccgttgg cgaagtcacc gaactgtgct tcccagatgg tcagggtgcg
    11281 tggttctgcg gtggcataac cgtattcaaa cgccagtacg gcttcttcag acagtacaga
    11341 gtcccagacg cggaacgcgc cctggccgtt atggatatgt tgcagcggcg tgtaagtgga
    11401 accgttagac tggttgtgga tcaccgcgtg gcggtggaag aaggtaccgc gaccggagtc
    11461 ttcacccgac aggcgaaccg gaatgccttc gtcaaccaac gtggcgtaag cgaggttttc
    11521 cgcgccgccc cagtcgaaca gtttctcacc ggctgccatc gcctggcgat cgccataaat
    11581 tttggcaacg cgagactgca tttcaaccgc ttccggcacc gtgctgatgc gtttagccag
    11641 ttcctgcagg cgcttcatct caactttgtt cgggtactct tcgtcccatt cgtggttgag
    11701 gtacggcgac caggtgaaag agtgcatgtt catcggacgc cactctgcta caacgcaatc
    11761 gccagcatcc agcgcatcgc ggtacaggtt aaccatctcg gtggcatctt ccagcgtcgc
    11821 gactttttcc tgctccagct tgtcagcgta gattttgcgc ggcgtcggat gttttttgat
    11881 tttctgatac atcagcggct gggttgcgct cggctcgtcg gcttcgttgt ggccgtgacg
    11941 gcggtagcat accaggtcga tgaagacatc acgtttaaag gtgttacgga aatcgagcgc
    12001 cagacgggtc acaaaggcaa cggcttccgg atcatccgca ttaacgtgga aaatcggtgc
    12061 ctgaaccatc ttaccgatat cagtacagta cggcgtcgaa cgggcatcca gcgggttaga
    12121 ggtggtgaag ccaacctggt tgttgataac gatacgtacc gtaccgccaa cttcataacc
    12181 acgcgctttc gacatgttca gggtttcctg aactacgccc tgcccggtca ctgcggcgtc
    12241 accatgaatg gtgattggca gcactttgtt gctgctcggc tcatcaagtc tgtccagacg
    12301 ggcacgaaca gaaccgataa cgaccgggct tacaatctca aggtgagacg ggttaaacgc
    12361 cagcgccagg tgcaccaggc cgccatcggt ctggaagtca gacgagaagc ccatgtggta
    12421 tttcacgtcg cccgtgccga ggtgttcttt atgtttaccg gcaaactcgt cgaacaagtc
    12481 ttgcggtttt ttacccagca cgttcaccag cacgttcaga cgaccacggt gcgccatccc
    12541 gagaaccact tcgcgggtgc cgctgttgcc agcgtggcgg atcatctctt taagcatcgg
    12601 gattaacgcg tcaccgcctt ccagcgagaa gcgttttgcg ccagggaatt ttgcgccaag
    12661 gtaacgttca aggccttcag cggcggtcag ttcgcttaag aagcgttttt tctcttcgct
    12721 attgaaagtc gcgcgaccag actcgatacg ctgttggatc cagcgttttt cttcggtgct
    12781 ggtaatgtgc atatactcgg caccaatcgg gccacagtag gtttgcttga gggcttccag
    12841 cagctcgccg agtttcatgg tttctttgcc gctggcaaat gaaccgacgt tgaaggtctc
    12901 ctggaagtct gcttcggtca gatcgtggaa agacggatcc agatcggcca ctttatcttg
    12961 ctgccacagt cccagcggat cgagattcgc atgctggtga ccacggaagc ggtatgcgtt
    13021 aatgagctgc aggactttaa cctgcttcac attggtgtca gggtcggaga tcgttgaaga
    13081 gtaacgtgaa gcgtctttcg ccaggcggcg gaaatattca cgcgtttgag agtggaattg
    13141 atccggtttg actcccgtac caggtaactg ctggaacgtc gaacgccagt tagcgtcaac
    13201 cgagtcagga tcggttaaga agtcttcata gagctgttct atccagctct ggtttgcgcc
    13261 agagaggtaa gaagagtcca accaggcttt caaagcgctg ttctgcatcg tgatccctta
    13321 agcatctttt ttatgcttac ttcgccgtgg atactaccac gcacatttca tatctgatgt
    13381 gcgtgccggg gttcacttgt tgcggacttg tcgtgttttc tgcgtccgct aaggaacctt
    13441 taaaaactgt cttagtgtca ggggcagttt ttaaagattt cctgcatttc ttttgtaggc
    13501 cggataaggc attcacgccg catccggcgc tgtttgcctg atgcgacgct tgcgcgtctt
    13561 atcaggccta cggtttctgc atttcttttg taggccggat aaggcgttca cgccgcatcc
    13621 ggcactggtt gcctgatgcg acgcttgtgc gtcttatcag gcctacggtt tacgcattac
    13681 gttgcaacaa catcgacttg atatggccga tggcgcgcgt cgggttcagc cccttcggac
    13741 atacactgac gcagttcatg atgctgtgac agcggaatac actgaatgca tcgctcaaac
    13801 cgtcgaggcg gctgtcagtc tcggtatcac ggctatcgat caggaaacga tatgccgcta
    13861 acaagcctgc cggaccgata aacttatcgg gattccacca gaaagacgga caagaggttg
    13921 aacaacatgc gcagagaata cattcataca acccgtcgag tttttcgcgc tgctctggca
    13981 tctgtaaatg ctcgcgagct ggcggatttt gtccattatt caacaggtaa ggcttaattt
    14041 tctcatattg cgcatagaat tgtcccatgt ctaccaccaa atcgcggatc accggtaaac
    14101 ctggcagcgg acgaatcaca atcttcttgc ccggctggtt gagtgccgaa atcggggtaa
    14161 tacaggccag accattctta ccgttcatgt tcagaccgtc ggaaccgcac acaccttcac
    14221 ggcaggagcg gcggaacgac aggctgggat ctttctcttt cagctgaata agcgcatcca
    14281 gcagcatcat gtcgcgacct tcttccgctt ccagggtgta atcctgcata cgcggagcat
    14341 catcaacatc cgggttatag cgataaattg aaaactcgag tctcattttc ctgtctccgc
    14401 attagtaagt acgaatcttc ggcgggaatg ccgggcgcag tttcggttcc atgttgacgc
    14461 ttcggcgcgt catggattcc gactctggca gatacaggga gtggcacagc cagttttcat
    14521 catcacgatc cgggaagtcg aagcggctat gcgcgccacg gctttcggta cggaagttgg
    14581 cagaaacagc cgttgcatac gccgtttcca tcaggttatc cagttccagg cactcaacgc
    14641 gctgggtgtt gaactcgctg gaagtgtcat ccagacgggc atttttcaga cgctcgcgga
    14701 taactttcaa ctgctcaagc cctttcgcca tcgcatcacc ttcacggaag accgagaagt
    14761 tatgctgcat acattcttgc agtgctttac ggatcgccac cggatcttca ccgttacggt
    14821 tattgttcca gcggttcagg cgatccagag acgcttctac atcagactcg ctggcatcgc
    14881 gcagtgcgcc ctgctcggcg atagactctt gcagatgcag acctgccgca cgaccaaata
    14941 ccaccaggtc cagcagcgag ttgccgccca gacggttagc gccgtgtacc gatacacaag
    15001 cgatttcacc aacggcaaac agccccggaa caaccacatc ttcgcctttc tcattcaccg
    15061 tcagcgcctg accggtcact ttggtcggaa taccgcccat catgtagtga caggttggga
    15121 taaccggaat cggctctttc accggatcga cgtgagcgaa ggtacgggag agttccagaa
    15181 tacccggcag acgggattcg agaacttctt tccccagatg gtcaagtttc agttttgcgt
    15241 gtggccccca cggaccatca cagccgcgac cttcacggat ttcgatcatg atggaacgcg
    15301 ccaccacgtc acggcccgcc aggtctttgg cgttcggcgc ataacgctcc ataaagcgtt
    15361 cgccatgttt gttcagcaga taaccgcctt caccacggca cccttcggtg accagtacgc
    15421 ccgcaccggc aataccggtc gggtggaact gccacatttc catatcctgc accggtacgc
    15481 ctgcacggat agccatgccg acaccgtcgc cagtgttaat gtgggcgttg gtggtggact
    15541 gataaatacg ccctgccccg ccagtcgcca gcactgtcgc gcgagcttta aagtaaacca
    15601 cttcaccagt ttcgatgcac agtgcggtac aaccgaccac tgcgccatcc tggtttttca
    15661 ccagatccag cgcataccac tcggagaaaa tggtggtgtg gtttttcagg ttctgctgat
    15721 aaagcgtgtg caacagtgcg tgaccggtac ggtcagctgc cgccgcagta cgtgccgcct
    15781 gctcgccgcc gaagtttttc gactgaccgc caaacggacg ttgatagata cgaccatcat
    15841 caagacgcga gaacggcagg cccatatgtt ccagttccag aatcgcttcc ggcccggttt
    15901 tacacatata ttcaatcgcg tcctggtcac cgatatagtc cgaccctttt acggtgtcgt
    15961 acatatgcca ttcccagtta tcttcatggg tattacccag cgcaacggta ataccacctt
    16021 gcgcagaaac ggtatgggaa cgggtcggga agaccttaga gagcagcgca caggtctggc
    16081 cactctggga aatttgcagc gccgcgcgca tacctgcgcc gccagcacca atcacaactg
    16141 catcaaattc tctgactggc aatttcatca cacaccccac accacaacga atccataaat
    16201 cacgtaaacc accagtgcaa cgacaatcac cagttgcagc atcaggcgca aggccagcgg
    16261 tttaacgtag tcggtcaaca cctgccacat gccgatccag gcatggatca agatagaaaa
    16321 cagcgccagc agggtgaaca ctttggtgaa cgcagaggcg aagaaaccaa tccagacttc
    16381 atatgtcagc tcgccactgg tagcgaaaaa accgaccata taaatgatgt agagcgtcag
    16441 gacgatagcg gtagcacgaa cgaggatgaa atcatgtacg ccattgcgtc ctaatgcgga
    16501 ggcgttgctt accatacgag gactcctgcg agaagtgaaa gcacgacagt aataacaaag
    16561 gagattttgg cggagcgttt acccgcttcg aatgtttctt ccagatagcc aaaatccatc
    16621 atcatgtggc gaatacctac gacgacgtga tatgccagag cggtaaggat gccccacatg
    16681 ataaatttga cgaagaagct gcccataatc gcggaagctt gctcgaaacc ttcaggggaa
    16741 gagaggctgg tacccagaag ccacagcagg atgcccactg caacaaaggt gatcacaccg
    16801 gaaacgcgat ggagaatgga cgctatcgcc gtgacgggga accggatggt ctgtaggtcc
    16861 agattaacag gtctttgttt tttcacattt cttatcatga ataacgccca catgctgttc
    16921 ttattattcc ctggggacta cgggcacaga ggttaacttt ctgttaccag gagacgtcgg
    16981 gatttccttc ctccggtctg cttgcgggtc agacagcgtc ctttctataa ctgcgcgtca
    17041 tgcaaaacac tgcttccaga tgcgaaaacg acacgttaca acgctgggtg gctcgggatt
    17101 gcagggtgtt ccggagacct ggcggcagta taggctgttc acaaaatcat tacaattaac
    17161 ctacatatag tttgtcgggt tttatcccga acagtgatct gcgtcacgat aacaacattt
    17221 atttaatttt taatcatcta atttgacaat cattcaacaa agttgttaca a
//
LOCUS       DACADF010000101        17215 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.103, whole genome shotgun sequence.
ACCESSION   DACADF010000101 DACADF010000000
VERSION     DACADF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.103"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..412
                     /gene="ecpR"
                     /locus_tag="ISB30_003213"
                     /gene_synonym="matA"
     CDS             <1..412
                     /gene="ecpR"
                     /locus_tag="ISB30_003213"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HAP1990817.1"
                     /translation="FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANY
                     WYYNSNIRGVVYAGLSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLP
                     KSIARIENCSVKTVYTHRRNAEAKLYSKLYKLVQ"
     gene            487..1074
                     /gene="ecpA"
                     /locus_tag="ISB30_003214"
                     /gene_synonym="matB"
     CDS             487..1074
                     /gene="ecpA"
                     /locus_tag="ISB30_003214"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HAP1990818.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1131..1799
                     /locus_tag="ISB30_003215"
     CDS             1131..1799
                     /locus_tag="ISB30_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HAP1990819.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1825..4350
                     /locus_tag="ISB30_003216"
     CDS             1825..4350
                     /locus_tag="ISB30_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990820.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4340..5983
                     /gene="ecpD"
                     /locus_tag="ISB30_003217"
     CDS             4340..5983
                     /gene="ecpD"
                     /locus_tag="ISB30_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HAP1990821.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            5952..6662
                     /locus_tag="ISB30_003218"
     CDS             5952..6662
                     /locus_tag="ISB30_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HAP1990822.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPSGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6969..7298
                     /locus_tag="ISB30_003219"
     CDS             6969..7298
                     /locus_tag="ISB30_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAP1990823.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7310..7473)
                     /locus_tag="ISB30_003220"
                     /pseudo
     CDS             complement(7310..7473)
                     /locus_tag="ISB30_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7547..8161)
                     /locus_tag="ISB30_003221"
     CDS             complement(7547..8161)
                     /locus_tag="ISB30_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HAP1990824.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8376..8561)
                     /locus_tag="ISB30_003222"
     CDS             complement(8376..8561)
                     /locus_tag="ISB30_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990825.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            8610..8768
                     /locus_tag="ISB30_003223"
                     /pseudo
     CDS             8610..8768
                     /locus_tag="ISB30_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            8995..9330
                     /locus_tag="ISB30_003224"
     CDS             8995..9330
                     /locus_tag="ISB30_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAP1990826.1"
                     /translation="MAILITQDRQVANLTPPEGKSRITVSVKSKVGSGLSVESRSGSQ
                     TKSWIYRPYLNGKQIKITLGSYPAMTLSQAREAHADALALVKQGIDPRYARKTEKQQN
                     EQMPIFSQL"
     gene            9538..10314
                     /locus_tag="ISB30_003225"
     CDS             9538..10314
                     /locus_tag="ISB30_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP1990827.1"
                     /translation="MTLDHAALQGLIDHNPARLLKPAMFGASMGKPRERWLPQDELQM
                     LWKALDEAGFGGGSVATGGRGIASSVILSQAIANALRLIILTGVRRSEAVNMRWEQIN
                     GNRWTIPETKNGKSHIVTLHPLALSLLKTQRIISEGGWVFESLSKPSFPVTGDAITRA
                     LERLKTKYMAEVAPFSPHDLRRSIATGCAEYLDAPERLIELLLNHVPKDRLIRTYQVG
                     QQAEKLRNLFLKWGDFIEHEIIESDNGTPDNVIQISFGKR"
     gene            complement(10479..11399)
                     /locus_tag="ISB30_003226"
     CDS             complement(10479..11399)
                     /locus_tag="ISB30_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP1990828.1"
                     /translation="MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPR
                     LSVDIDLAYKSFADRDTDLAAINDALKRITASLNGRPGITAIRQENKADEKRIIVNTA
                     DAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
                     PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSG
                     MTQQETSLESLLSVTTLLPQALKSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAI
                     RWKQRNLAMLKDKNPAKYVAAVNKLERVLE"
     gene            complement(11386..12189)
                     /locus_tag="ISB30_003227"
     CDS             complement(11386..12189)
                     /locus_tag="ISB30_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990829.1"
                     /translation="MSIQERHLPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYV
                     QNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLTSLALQGHAHYTEPGK
                     PQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPE
                     LAAYEIACGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNG
                     HPYFQYLDQSGIETGTGKRQIIPGGWLEPDYQITVPRTFKPEGVEDGSA"
     gene            12452..15550
                     /locus_tag="ISB30_003228"
     CDS             12452..15550
                     /locus_tag="ISB30_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP1990830.1"
                     /translation="MKFTEDTRVKIPVILHLIRLGYHYLSLKEQRWDKETNIFPDLFT
                     AAIGRINPGLAPDDIGRLLKDLTLLLDNDDLGRAFFEKLSDRSGIKLIDFDNFNNNSF
                     HVVTELTCENGDEEFRPDITLLINGMPLIFIEVKKPNNREGIVAERNRINERFKNPKF
                     KRFANITQFMIFSNNMEYDDSDPEPIQGAFYASSSYSNPVFNYFREEELFNLTNLLKP
                     LSEETEIAVLKDNNLEVIRTNPEFQTNKDHNRPTNRLCTALLSRDRLAFILRYALVYV
                     AEPGGIQKHIMRYPQLFATKAIERKLNAGVKKGIIWHTQGSGKTALAYYNVRFLTDYF
                     QNQQVIPKFYFIVDRLDLLQQAQREFTARGLIVHLIDSREAFTRDIKATQALHNHHGK
                     AEITVVNIQKFRDDPDVVTTKDYDINIQRVYFLDEVHRSYNPKGSFLANLSQSDTNAI
                     KIGLTGTPLLGTDYNSRDLFGDYIHKYYYNASIADGYTLRLIREEIATNYRMILQQAL
                     KEVEVRMGDVDRKYIYAHPSFVKPMLNYIVTDFAKSRGALNDDTIGGMVICDSSEQAK
                     QMFEIFNTDYAESPTAADEVSEEMPVLQAAEPAAVYHLHAKETRKIKRAALILHDIGS
                     KQERKEWVEQFKAGKIDLLFVYNMLLTGFDAKRLKKLYLGRVIRKHNLLQALTRVNRT
                     YNHFRYGYVVDFADIRQEFDATNKAYFEELQAELGDEMEHYSRLFKSAEEIREEIEHI
                     KDVLFSFDTENAEIFTDQINQIQDRETALALKNALADARSLYNLIRLQGNTEFLQALD
                     FNKLNILYREASRRLDLLNLKADLEQGVDLSGLLNRALEEVIFEFHKVGEEELVLADE
                     LKETLRRTREALAANFDQQDPQFVSLKDELERLFKKKKLSEVTQQEMVANIGTLNKIH
                     GRIKELNRQNNLLRQKYRGDVKYARTHKRLRERGGLSEPERKIFEALLSVKLDADEKV
                     LNNSQILNNESYFERHMESCVITHFEDEHHITLTSSAVSDINRLIVAEYLNEFNTGAR
                     TW"
     gene            15544..17181
                     /locus_tag="ISB30_003229"
     CDS             15544..17181
                     /locus_tag="ISB30_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HAP1990831.1"
                     /translation="MVELEFRDKTKALIDSLKSICANYGLGNDGNEFKIITQAFLYKF
                     LNDKFAFEAKQKDKSIASAESWEDALSAMSEDQLKKLQQRMAPDTARLKPHHFIRYLY
                     NRQNAADFARTFDDTLMDIAATNNDVFAVKTDGGAKVVLFERLSQYIADESKRDDFCR
                     AIINKLADFSFERIFTQKFDFYATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMAEIL
                     VPKAQQGVVRNVSCYDPSAGSGTLLMNVAHAIGEDRCSIFAQDISQKSSSLLRLNLIL
                     NNLVHSIPNVIQGNTILHPFHKDGGALKRFDYIVSNPPFKMDFSDFRDALDSKENQQR
                     FFAGIPKIKAKARDKMEIYQLFLQHIIFSLKPGGKAAVVVPTGFITAQSGIDKGIREH
                     LVQNKMLAGVVSMPSNIFATTGTNVSILFIDASNKEKVVLIDASNLGEKVKDGKNQKT
                     VLTECEEKRICEAFNNKWSEEDFSVVVSYDDIAAKNYSFSAGQYFDVKIEYTDMTPEQ
                     FAAKMKGFTENLNNLFEQSRELEVQIKMQMADIKYEI"
BASE COUNT         4705 a         4540 c         4198 g         3772 t
ORIGIN      
        1 tttcgtgttt atcaatctca atacggattc tttaactgag tttataaacc gtgataatga
       61 gtggttatcc gcggtaaagg ggaaacaggt cgtattgatt gcggccagaa agtcagaagc
      121 cttagcaaat tattggtatt acaacagcaa tattaggggc gtggtatacg ctggactgag
      181 tcgtgatatt agaaaagaac tggcctatgt gattaatggc aggttcctga gaaaagatat
      241 taagaaagat aaaatcactg accgggaaat ggaaattatc cgcatgacgg ctcagggaat
      301 gctgcctaaa tcgattgcca gaattgaaaa ttgtagtgtg aagacagtgt atacccatcg
      361 gcgtaatgca gaggccaagc tgtactcaaa attatataag ttggttcagt aaactccagg
      421 caagttagtt ttaaaaaatg actcaccggg acttcatgtc ctcaattcaa ctcgggaaga
      481 aaagcaatga aaaaaaaggt tctggcaata gctctggtaa cggtgtttac cggtacaggt
      541 gtggcgcagg ctgctgacgt aacagctcag gctgtagcga cctggtcagc aacagccaaa
      601 aaagacacca ccagtaagct ggttgtgacg ccactcggta gcctggcgtt ccagtatgcc
      661 gaaggcatta aaggttttaa ctcacaaaaa ggtctatttg acgtggctat cgagggtgac
      721 tcaacggcta ccgcctttaa actgacctca cgtcttatca ccaacacctt aacccagttg
      781 gatacctcag gttccacact gaatgtgggc gtggattata acggcgcggc agtcgaaaaa
      841 actggcgata ccgtgatgat cgataccgcc aacggcgtac tgggcggcaa ccttagccca
      901 ctggctaacg gttacaatgc cagcaatcgt accactgcac aggatggttt cactttctcc
      961 atcatcagcg gcaccaccaa tggtaccacc gcagtaaccg attacagcac tctaccggaa
     1021 ggcatctgga gcggcgacgt tagcgtacag ttcgacgcta cctggaccag ttaatctctc
     1081 tgatgtacca gcaggggtac ccccctgctt tattcctgga cggaattgtt atgaaaaagc
     1141 accttctgcc tctcgctctg ctgttttccg gaatatcacc ggcccaggcg ctggatgtcg
     1201 gcgatatatc atcgtttatg aacagtgaca gcagcacgct gagcaaaacg atccaaaaca
     1261 gtaccgacag tggccgcctc atcaatatcc gtctcgaacg gctctcttca ccgcttgacg
     1321 acgggcaggt tatcgcaatg gacaagccgg atgagttgct actcactccc gccagcttgc
     1381 tgctacccgc ccaagccagc gaagtgatcc gcttcttcta taagggacca gcagatgaaa
     1441 aagagcgcta ctaccgcatt gtctggtttg atcaggccct cagtgatgcg cagcgcgata
     1501 atgccaaccg cagcgctgtg gccactgcct ccgcccgcat cggcaccatc ctggtcgtcg
     1561 caccccgcca ggcaaactac cactttcagt acgccaacgg ctccctgaca aatacaggaa
     1621 atgcgacgct gcggatcctc gcctacggcc cctgcctgaa agccgccaat ggtaaggagt
     1681 gtaaagagaa ttactacctg atgccgggca agtcgcgtcg ttttacccgc gtggacacgg
     1741 cggataacaa aggacgggtt gcactttggc agggtgataa gttcattccc gtgaaataga
     1801 tagctgtgca gatggataac gacaatgcct ttacgacggt tctccccagg actgaaagcc
     1861 cagtttgcct tcggcatggt ctttttgttc gttcagcccg atgccagcgc tgctgacata
     1921 agtgcgcagc aaataggtgg ggtgattatt ccgcaggcct tcagtcaggc gctccaggac
     1981 ggcatgagcg tcccgcttta tattcatctc gccggtagcc agggtcgcca ggacgatcag
     2041 cgaatcggca gcgcttttat ctggttggat gatggacagt tacgcatccg aaaaatacag
     2101 ctggaagaga gtgaagataa cgccagtgtc agcgaacaaa ctcgacagca gctgatgact
     2161 ctggccaacg ccccgttcaa tgaggccctt accatccccc tgactgacaa cgcgcagttg
     2221 gatctcagct tgcgccaact gctgctgcag ctggtggtca agcgcgaagc gctgggcacc
     2281 gtactacgct cacgtagcga agacatcggg cagtccagtg ttaacaccct cagcagtaat
     2341 ctgagctata acttcggcgt ctataacaac cagttgcgta acggcgggag caacacctcc
     2401 agctatctgt cgctgaataa cgttactgcg ctgcgcgaac atcacgtggt gctcgacggc
     2461 tcactgtacg ggattggtag cggtcaacag gacagtgaat tatataaagc gatgtatgaa
     2521 cgcgattttg ctggtcaccg atttgccggt ggaatgctcg acacctggaa cttgcagtcc
     2581 ttagggccga tgaccgccat ttcagcaggg aagatttacg gcctttcctg gggaaaccag
     2641 gccagctcca ccatcttcga cagcagccag tcggccacgc cagtgatcgc ctttttaccg
     2701 gcggcgggcg aagtacatct cacccgtgat gggcggctac taagcgttca gaacttcacc
     2761 atgggcaatc atgaagtgga tacccggggt ctaccgtacg gtatttacga tgtggaagtt
     2821 gaggtaatcg ttaacggtcg cgtgatcagc aaacgcaccc agcgggtcaa taagctgttt
     2881 agccgggggc gtggcgtcgg tgcaccgctg gcgtggcaga tatggggcgg tagctttcat
     2941 atggatcgct ggtcggaaaa cgggaaaaag acgcgaccag ccaaagagag ttggctggca
     3001 ggtgcctcga cctccggctc actgagtacg tttagctggg cggcaacggg atatggatac
     3061 gataatcagg cggtgggtga aacccgtctg acgcttccgc ttgggggggc gatcaacgtt
     3121 aacttgcaaa acatgctggc cagtgacagc tcatggagca acatcgccag catcagcgcc
     3181 actctacctg gaggcttcag ttcgctgtgg gttaaccagg aaaaaacccg cattggcaat
     3241 caattgcgac gtagcgatgc cgacaaccgt gcaatcggcg gcacactcaa cctgaactca
     3301 ctgtggtcga agctgggtac gttcagcatc agctacaatg atgaccgccg ctacaacagc
     3361 cactattaca cggcagatta ctatcaaagt gtctacagcg gtacctttgg ttcgcttggc
     3421 ctgcgggccg gtattcagcg ctataacaac ggcgacagca gcgccaatac agggaaatat
     3481 atcgctctcg atctctcgct accactgggc aactggttta gcgcagggat gacccatcaa
     3541 aacggctaca ccatggcaaa cctgtcagca cgcaaacagt ttgatgaagg aaccattcgc
     3601 actgttggtg ccaatctgtc acgagccatc tccggcgata ccggtgatga caaaaccctc
     3661 agcggtgggg cgtatgcaca gttcgacgct cgttacgcca gcggaacgct gaacgtcaat
     3721 agcgcggcgg acggctacat caatactaat ttgaccgcca acggcagcgt cggctggcag
     3781 ggtaaaaaca tcgctgccag cgggcggacc gatggcaacg ctggggtgat attcgacact
     3841 ggcctggaga acgatggtca gatcagcgcc aaaatcaacg ggcggatttt cccgcttaac
     3901 ggcaagcgta actatctccc gctctctccc tatggaagat atgaggtgga gttgcagaac
     3961 agcaaaaact cactcgacag ttacgatatc gtcagcggtc gcaaaagtca tctgactctc
     4021 tatccaggca atgtcgctgt cattgagcca gaggtgaagc agatggttac cgtctccggt
     4081 cgtatccgtg cggaagacgg cacactgctg gctaacgcac ggattaacaa ccatatcggc
     4141 cgaacccgaa ccgatgaaaa cggcgagttt gtcatggacg tggataagaa ataccccact
     4201 atcgattttc gctacagtgg caataaaacc tgcgaagtgg cactggaact caaccaggcg
     4261 cgcggtgccg tctgggtcgg tgatgtggtc tgcagcggcc tctcatcgtg ggcggcggtg
     4321 acgcagacag gagaagagaa tgagagttaa cctactgata gcgatgataa tctttgcgct
     4381 aatctggcct gcaaccgcgc tcagagcggc agtgagcaaa acaacctggg cggatgctcc
     4441 ggcacgcgag tttgtgtttg tcgaaaacaa ctcagacgac aactttttcg tcactcctgg
     4501 cggggcgctg gatccgcgcc tgaccggtgc caaccgctgg accggtttaa aatacaatgg
     4561 ttcaggaacc atctatcagc aaagcctcgg ctacattgat aacggttaca acaccggcct
     4621 ttataccaac tggaagtttg atatgtggct ggaaaattca ccagtttcat ctcctttaac
     4681 tggcttgcgc tgcatcaact ggtacgcagg gtgtaatatg accaccagtc ttatcctgcc
     4741 gcaaaccacc gacgccagtg gattttatgg cgcgacggtg accagcggcg gcgcgaagtg
     4801 gatgcacggc atgttgtcag acgcgtttta ccagtatctg caacaaatgc ccgtcggcag
     4861 cagctttaca atgaccatca atgcctgcca gacctctgtg aactatgacg ccagcagcgg
     4921 cgcacgctgt aaggatcagg cctccggcaa ctggtatgtt cgcaacgtca cccatacgaa
     4981 agcagcaaat ctgcggttga taaataccca ctcgctggcg gaagtattta tcaacagcga
     5041 cggagtaccg actctgggcg aagggaacgc cgactgccgg acgcaaacca tcggcagccg
     5101 ttcaggatta agttgtaaga tggttaacta taccctgcaa acaaacggac tcagcaacac
     5161 ctcaatccat atattcccgg cgatcgccaa ctcgtcgtta gcctcggccg tcggggcgta
     5221 cgatatgcag ttcagtctga atggcagttc atggaaaccg gtgagcaata ctgcctatta
     5281 ctacaccttc aacgagatga agagcgcaga ctcgatctat gtgttcttct cgagcaactt
     5341 ctttaagcag atggtgaacc tcgggatcag cgatatcaac accaaagatc tgttcaactt
     5401 tcgctttcag aacaccacat caccggagtc tggctggtat gaattttcta cctccaacac
     5461 gctgattatc aaaccccgtg atttcagcat cagtattatc tccgatgaat atactcagac
     5521 accgtcgcgg gagggatatg tcggcagcgg cgagtcggca ctcgatttcg gctatatcgt
     5581 aaccaccagc ggtaaaacag ctgccgacga agtgctgatc aaggtgaccg gacccgcgca
     5641 ggtgattggc gggcgctcct attgtgtctt cagctccgat gacggtaagg cgaaagtacc
     5701 gttcccggcg acgctttcct ttattacccg caacggagct acaaaaacct acgatgccgg
     5761 gtgcgatgat agctggcggg atatgaccga tgcgctgtgg ttgaccacac cgtggactga
     5821 tatctctggc gaagtggggc agatggataa gaccacagtc aaattttcga ttccaatgga
     5881 taacgccatt tctctgcgta cggtagatga taacggctgg tttggcgaag tcagcgcttc
     5941 aggagagatt catgttcagg cgacgtggcg taacattaac taaggccctg ctgacagtgg
     6001 tctgtatgct ggcggcacct ttgacacagg cgatttcggt cggcaatctg actttttcac
     6061 tgccgtccga gactgacttt gtcagcaaac gtgtcgtgaa taacaacaaa agcgcacgga
     6121 tataccgtat tgccatcagt gctattgata gcccgggcag cagtgaattg cgcacccgac
     6181 cggtggatgg tgaactactt ttcgcccccc gccagctggc gttgcaggct ggtgagagcg
     6241 agtattttaa attttactat catggcccac gggataaccg cgagcgctac taccgggtct
     6301 catttcgcga ggtccccact cgtaacctga caaaacgcag cccaagcggc ggtgaggtca
     6361 gcacggagcc ggtggtggtg atggatacca ttctggtagt acgaccacgt caggttcagt
     6421 ttaaatggtc cttcgatcag gtgacaggaa cggtaagtaa caccggcaac acgtggttta
     6481 agctactgat taaaccagga tgtgattcga ccgaagagga aggcgatgcc tggtatctac
     6541 gtccgggaga cgtggttcat cagcctgagt tacgtcagcc ggggaatcat tatctggtct
     6601 ataacgacaa attcattaag attagcgatt cctgtccggc taagccccct tcggcggact
     6661 aagacttatc catcggacga gggaagaatt ccacctcaga gactcaaaaa tcgtttaaat
     6721 gatgtggaag cgatcgtcaa tgtcaccgat gagctttctc atatgcacac cacagtgttc
     6781 aggagacacg gaaaggtacc aggcggcctg ctaacgagtg gtttcttcac taattaggac
     6841 gtaaaatcgg taacggctgg aaatcattca atactcacac tatcgaaagt acaccagcca
     6901 accgcgatac gtccctgcat acgacgtacc gcagttatct ttacgattta tagctgtact
     6961 ggtgaattat gagcaatttg aatccatgca tgacgtgtgg tgcctgttgt gcatttttcc
     7021 gcgtctcttt ttactgggcc gaagccgacg atgctggcgg taccattccc gccaggctca
     7081 ctgaacaaat atcccctttt caccgatgca tgagcggtac taatcagaaa aatccccgat
     7141 gtattgccct tgcaggaaca ccgggcaaaa atgcctgctg cacgatatat gaaaatcgat
     7201 cgtccacatg cagagaattc gccatgtctg gtgagaacgg agaagtcaat gaggcttgca
     7261 atcgtgcaag agctaaatac gggctaacac cgttataaat atacaataat taattgcatt
     7321 gccccgccag ctgtaatagc ggggcttcgt ttttcaggga caacaaaacc tgaaacttct
     7381 tccattttcc agcaacaatg cctcttcttc tgtagcgtcc tccggatcta catcatcctg
     7441 aagtagcgcg atatgatcac tgactgtccg catcagaata tgaaaagcaa cgtttttaac
     7501 ctataaaaat ggcgctatat ttgcgccatt tttatcattc aaagcattat ctatttgagc
     7561 ctaaagggat ctcaggatct ggctcatggg taattctgtt tcgtaaatct ctgcgaataa
     7621 tttcaataga ccagaaccag actaaatgtc caaaaatttc agagacattc tcataccacg
     7681 gaagatcaaa gagaggtggc gtaagtccca tgagagggaa tgaaatcata tgaacaaaaa
     7741 gttgcgctaa agcacctgcc agtaagccct gccaaagttt aattttcgga aatacttcgg
     7801 cgaccacaca gtaaccaacg gcaaaaacta tcgaaaagat tatgtgcgtt acaccaaccc
     7861 agttaaagac atgcccggcg aaggtataaa cagccgaatt tggatctgtc agtcccaacc
     7921 aatcccgaag aaaaatgtac ggaggattga gaaagttacg ggagcaatca atttggcctg
     7981 cagccctgat taatgattcc ggaccacacg ctgcattaaa catatcaacc ggactacgtg
     8041 gtggcaatgg aacttctgcc ccccacttaa caaatgcgga aacaacgcca gcaatcagcc
     8101 caatgaatgc agcaagacca taacgtctgc ggtttggtgg agtttgttca aatatattca
     8161 tatctaccct gcttgtacca ttatgttata cacctcttta ggagtattca taaaacaagg
     8221 caaatgtaaa gaactgtatt gttttgtata accacatggt tacctaatcg ccaattaata
     8281 taagctccat tatttctcca tatttttatg ttagaagtga caaagagaaa tgtaatgttt
     8341 ctctcagaga atataaatca ttccttttgc ccaccttaat ggtgggcttt tttatgcaaa
     8401 acctgaacgt cctgtggtat tcctctggca taatgatttt cgcgattaac caacaaccag
     8461 accagcaaag acgccaccac aagcacagta acatccatca ggcttgccac accccatgcg
     8521 tgcggctcaa agccgccatg gcatattaat ccgcttgcca ttttcgaaca gctgattggt
     8581 ttggtccggt gtttccctgc ttgcacttaa aaatgagcgg ataggaaact ctcgaaatgg
     8641 ttcgtcgata tgctcacctc gcacctaatc accttaccga acacgcacgg taaatagatt
     8701 cgatcctgaa cccatcggtc ccaaatctgt cccaatcaaa aaataaggaa ggtattaatg
     8761 atgtgtaact tattgattta agtggtgccg ataataggag tcgaacctac gaccttcgca
     8821 ttacgaaacc aaaaagcatc tatttttccg catatctaca cgtctctata aatctcataa
     8881 aaatattaaa aatcatataa ttaaacaaaa aacccatctt atattgttcc atcgaatttc
     8941 gctctataca atgctaaaca agtacgccag caagtacgcg agatgaggcc aaaaatggca
     9001 atattgataa cccaggacag gcaagtcgca aacctgactc cccctgaagg aaaaagtcgg
     9061 ataaccgtca gcgtcaaatc aaaagtaggt tccggcctgt ctgtcgaatc ccgttccggg
     9121 agtcaaacga agagctggat ttaccgccct tacctcaacg gaaaacagat taaaataacg
     9181 ttaggctcct accctgcaat gacattgtct caggcgcgtg aagcacatgc agacgcgctg
     9241 gcgctggtta aacaaggtat tgatccgcgt tatgcgcgaa aaacggaaaa acagcaaaat
     9301 gaacaaatgc cgattttttc acaattatga aaaaactggc tggcattccg tgaaaccaat
     9361 aaacccattg gggcgcgtac tctggctgat tatgagggaa catatcgccg ccatctggag
     9421 aaaggtttag gttcagtacg cgtctgcgac ctgtccagag cctacattta tgaacatctc
     9481 atacggatca gaaagaccag cgccgaaggt gtccgcaagg gacttattct tctcaatatg
     9541 acgcttgacc atgccgcttt gcagggattg atcgatcaca accctgctcg cttgcttaaa
     9601 cccgcaatgt ttggtgcctc aatgggaaaa ccgcgtgagc gttggttgcc acaagacgag
     9661 ttgcaaatgt tatggaaggc actggatgaa gcggggttcg gcggcggatc tgtcgcaacc
     9721 ggagggcgag gcatcgcgtc cagcgttatt ctttcacaag cgattgctaa cgcattacgc
     9781 ctcatcatcc tgacgggggt gcgacgttcc gaagcggtga atatgcgttg ggagcagatt
     9841 aatggcaacc gctggacaat acctgaaacc aaaaacggta aaagtcatat cgtgacgcta
     9901 catcctctgg cgctttctct attgaagact cagcgcatta tttcagaagg cggctgggta
     9961 ttcgagtcgc tcagtaagcc aagttttcct gtcaccggtg acgcaataac tcgtgctctt
    10021 gaacgcctga aaacaaaata tatggcagaa gtcgctcctt tctcaccaca tgatttacgc
    10081 cgcagcattg ctacaggctg cgctgaatat ctggacgcac cagaacggct tatagaactt
    10141 ctgttaaatc atgtgcccaa agacaggtta atcagaacct accaggtcgg gcagcaagcg
    10201 gaaaaattac ggaatctgtt tctgaaatgg ggggatttta ttgagcatga gatcattgag
    10261 tctgataatg gtactccaga taatgttatt cagatcagct ttggaaagag atagtttcgt
    10321 attttcatga gtagagatcg tctgataact cagcgatctc ttactcgatg gtgctatgta
    10381 ttgaattaaa tgaatatgtt tgagttattg ccacgaagtt attcatataa ccaaagcatc
    10441 atggcccaat tcagagtccc tgaacagcgc taagaatatt actccagcac cctctctagc
    10501 ttattcaccg ccgccacata cttcgccgga tttttatctt taagcatcgc cagattgcgt
    10561 tgcttccagc gaatagccgg aagatgctga atttcaggat agcgataccg cgaccagtca
    10621 gggctgtttt gcttaaaact gagcaagaac tcgcgatcca gatccgtaaa atgtgacttc
    10681 aacgcctgcg gcagcaaagt cgttactgac agaagcgatt ccagcgacgt ttcctgctga
    10741 gtcatccccg aaaattcctg agcgtataac gtggcaatcc gatctgtatc ccagtttgaa
    10801 gccagcaact cggcaatggg acggggatgg cctgccagat aacaaagaaa accgtcaaaa
    10861 atttcgcgcg tcaggcccgg tctccccagc atattcaaca catcaaacag atcgcgcgga
    10921 tgctgacgat caagcgcagc acatatttta ccgccataaa gatccgccag cgagacgaca
    10981 ctcatggtgg tgaagccata ctccatttcc acacgttcgc acacaggcat ttctgccgga
    11041 ggcaacaaca gcccgcgcca gaccgggcta acctcgatct taatctttgc atcagcggta
    11101 ttcacaatga tgcgcttttc atccgcttta ttttcctgcc gaatcgcggt gatccccggt
    11161 ctgccgttca gcgatgcagt aattctcttc aaagcgtcgt tgatcgccgc cagatcggta
    11221 tcgcgatccg caaagctctt atacgccaga tcgatatcca ccgatagacg ggggaaatcc
    11281 tgcacaaata aattgattgc tgtgccgcct ttcaacgcga aacaggattc cttcgccaca
    11341 tgcggcagag cggccatcaa cagtttaacc tgcctggcat agctatcacg cagatccatc
    11401 ttcgactcct tccggtttaa atgtgcgggg aacagtaatt tgataatccg gctccagcca
    11461 gccaccaggt atgatttgcc gctttcccgt acctgtttct ataccgcttt gatcaagata
    11521 ctgaaaataa gggtgtccat tacgccgggc aaggtaaagc atcacccggt tggttttaat
    11581 cgaatgactg gcgcgaagca gtgcctgcaa tttgcgtggg ctgagaagat tccccccctg
    11641 aaacagagca tcggcatgtt cgaacgagat caacgaagga acgccgcagg cgatctcata
    11701 cgcggccagt tccggtgcac tgcctgctaa ctgccggtcg ccaattttca gttccgtagt
    11761 aaatgacgag acatcaacgg taaggcccga tgtatacagc gtgataaatg ttgctgattg
    11821 ttcgaaggca gcaaaccatc ccggcagttt gacgtaagcc ggaagcgcga gccagacctg
    11881 cggcttaccc ggttcggtat agtgcgcatg gccctgaagc gcaaggctgg ttagccccgc
    11941 cacccgaaca ggcttttccc actgtgtttg aaggcagtaa accgcgtcta cccaatccgg
    12001 ctcacggccc gtccggcagt acacaccgtg agcgatgcgc ctgagccagc cattctgaac
    12061 atagagataa gaaagcttac gatctacgcc attgcgggta agccatgact ggagcagcac
    12121 ctgaccgggt agcgtgtttt gcatcaacca gtttagatag tttggcaaat gtcgctcctg
    12181 aatagacatt agtagaaata tttaaaccag tgtaggttca gggatacgcc acagcaagaa
    12241 atagtttaat gattcctcat ttcgtccact taaaatcgac attgagcaaa aaatacaaac
    12301 ttacctacat tcaggcaaag tgcgacaaat agactcggtc ggtttaaaga gaacgtgtaa
    12361 catagatgca aatcgaacag acattgttgc cagccgttcc aaggttgtgg ggcgctgcat
    12421 ggcctgagac gtgataaata cagggaacac aatgaaattc actgaagata cccgagtaaa
    12481 aatccccgtc atcctccacc tgatacggct gggctaccac tacctgtccc ttaaagaaca
    12541 acgctgggac aaagaaacca atatcttccc tgacctcttc accgctgcga ttggcagaat
    12601 aaatcccgga cttgcgccag acgacatcgg acgcctgtta aaagacctca cgctgcttct
    12661 cgacaacgat gatctgggcc gcgccttctt cgaaaaactc agtgaccgtt ccggtatcaa
    12721 actgatcgac tttgataact tcaacaacaa cagtttccat gtcgtcaccg aactgacatg
    12781 cgaaaacggt gatgaagaat tccgcccgga catcacgctg ctgatcaacg gcatgccgct
    12841 aatctttatc gaagtgaaaa aacccaacaa tcgggaaggc atcgtcgcag aacgcaaccg
    12901 catcaacgaa cgctttaaaa accccaaatt caaacgcttc gccaacatca cccagttcat
    12961 gatcttctcc aataacatgg agtatgacga cagcgacccg gagccgattc aaggggcatt
    13021 ctacgccagc agctcttaca gcaacccggt attcaactac ttccgcgaag aagaactctt
    13081 caacctgacc aacttactga aaccgctgtc agaagaaacc gaaatcgccg tcctgaaaga
    13141 caacaacctg gaagtgatcc gcaccaaccc ggaatttcag accaacaaag atcacaaccg
    13201 cccgacaaac cgcctctgca ccgcgctgtt gagccgggat cggctggcat ttatcctgcg
    13261 ctatgcgctg gtgtatgtag ccgaaccggg cggcatccaa aaacacatca tgcgctaccc
    13321 gcagctcttc gccaccaaag cgattgagcg caagctgaac gcgggtgtga aaaaaggcat
    13381 tatctggcac acccagggca gtgggaaaac cgcattggca tactacaacg tgcgcttcct
    13441 gacagattac ttccagaatc agcaggtgat cccgaaattc tactttattg tcgatcgtct
    13501 tgatctgctc cagcaggcgc agcgtgaatt caccgcccgt ggcttaatcg ttcaccttat
    13561 cgactcccgc gaagccttca ctcgcgatat caaagcaacg caggcgctgc acaaccacca
    13621 cggcaaagcg gaaatcaccg tcgtcaacat ccagaaattc cgcgatgacc cggatgtcgt
    13681 caccaccaaa gactacgaca tcaacatcca acgcgtgtac ttcctcgatg aagtccaccg
    13741 cagctacaac cccaaaggga gcttcctggc gaatttaagc cagtcggaca ccaacgccat
    13801 caaaattggc ctgaccggta cgcctctgct gggcacagac tacaactccc gcgacctctt
    13861 cggcgactac atccacaaat actactacaa cgcctccatc gccgacggct acacgctgcg
    13921 cctgatccgc gaagagatcg ccaccaacta ccgaatgatc ctgcaacagg cgctaaaaga
    13981 ggtggaagtc agaatgggcg atgtggatcg caaatacatc tacgcccatc ccagctttgt
    14041 taaaccgatg ctgaactaca tcgtcaccga ctttgccaaa agccggggcg cgctgaatga
    14101 tgacaccatc ggcggcatgg tgatttgcga cagctctgaa caggccaagc agatgtttga
    14161 gatattcaac actgactacg ccgaatcgcc caccgccgcc gatgaagtct ccgaagaaat
    14221 gccggtatta caggccgcag aacctgccgc cgtttaccac ctccacgcca aagaaacgcg
    14281 gaaaatcaaa cgcgccgccc tgatcttgca cgacatcggc agcaagcagg agcgcaaaga
    14341 atgggtggag caattcaaag cgggcaagat tgacctgctg ttcgtttaca acatgttgct
    14401 caccggcttc gacgccaaac ggctgaaaaa actctacctc gggcgggtga tcagaaagca
    14461 caacctgctc caggcgctga cccgcgtcaa ccgtacgtac aaccacttcc gctacggcta
    14521 cgtggtggac ttcgccgaca tccgccagga atttgacgct accaacaaag cctacttcga
    14581 agagctacag gctgaacttg gcgacgaaat ggagcactac tcccgcctgt tcaaatcggc
    14641 ggaagagatc cgcgaagaaa tcgaacacat caaagacgtt ctgtttagct tcgataccga
    14701 aaacgcggaa atcttcactg accagataaa ccagatccag gaccgggaaa ccgcgctggc
    14761 gctaaaaaac gcgctggccg acgcccgcag cctgtacaac ctcatccgcc tccagggcaa
    14821 cactgagttt ctccaggcgc tggatttcaa caaactcaac atcctttatc gcgaagccag
    14881 ccgccgcctc gacttactca acctaaaagc ggatctggaa cagggcgttg acctctccgg
    14941 cctgctgaac cgggcgctgg aagaggtgat ctttgaattc cataaagtgg gtgaagaaga
    15001 gctggtactg gccgacgagc tgaaagagac gctgcgccgc acccgcgaag cgctggccgc
    15061 caacttcgac cagcaggacc cgcagttcgt cagcctgaaa gacgaactgg agcgtctgtt
    15121 taagaaaaag aaactcagcg aagtcaccca gcaagaaatg gttgcgaata tcggcacact
    15181 caataaaatt catggccgca tcaaagagct aaaccgccaa aacaacctgc tgcgccagaa
    15241 ataccggggc gacgtgaaat acgcgcgtac ccacaaacgc ctgcgcgagc gcggcggcct
    15301 gagcgagccg gagcgtaaaa tcttcgaagc actgcttagc gttaagctgg atgcagatga
    15361 aaaagtgctg aacaacagcc agatactgaa caatgaaagc tattttgagc ggcatatgga
    15421 gtcatgcgtc atcacgcact ttgaggatga gcaccatatc actctgacct ccagtgcggt
    15481 aagcgacatc aaccgcctga ttgtggcgga atacctgaac gaatttaata ccggagcacg
    15541 aacgtggtag aactggaatt tagagacaaa accaaagccc tgatcgacag cctgaaaagc
    15601 atctgtgcga actacggcct gggcaacgac ggtaacgaat tcaaaattat cactcaggca
    15661 ttcctgtaca aattcctgaa cgacaaattc gcttttgagg ccaaacagaa agataaaagt
    15721 atcgccagcg ccgaaagctg ggaagatgcg ctcagcgcaa tgagcgaaga tcaactgaaa
    15781 aagctgcaac agcggatggc cccggatacc gcccgcctga agccgcacca ttttattcgt
    15841 tacttgtata accgacaaaa cgcagcggac tttgccagaa cttttgacga caccctgatg
    15901 gacatcgccg ccaccaacaa cgacgtcttc gcggtcaaaa ccgacggcgg cgcgaaagtg
    15961 gtgctgtttg agcgcctcag ccagtacatc gccgacgaat caaagcgcga tgatttctgc
    16021 cgcgccatca tcaacaagct ggccgatttc agcttcgaac gcatcttcac gcagaaattc
    16081 gacttctacg ccaccatctt tgaatacctg atcaaagact acaacagcaa ctccggcggc
    16141 aaatacgcgg aatactacac gccccacgcc gtggcgcgca tcatggcgga aatcctggtg
    16201 ccgaaagcgc agcagggcgt ggtgcgcaac gtaagctgtt acgacccgtc cgccggttcc
    16261 ggcacgctgc tgatgaacgt ggcgcacgcc attggggaag atcgttgcag catctttgcc
    16321 caggatatct cgcagaaatc ctccagcctg ctgcgtctga acctgatcct caacaacctg
    16381 gtgcattcga tccctaacgt tatccagggc aacactattc tgcatccgtt ccacaaggac
    16441 ggcggcgcgc tgaaacgctt tgactacatc gtctccaacc cgccgtttaa gatggatttc
    16501 agcgactttc gtgacgcgct ggacagcaag gaaaaccagc aacgtttctt tgccgggata
    16561 ccgaaaatca aagccaaagc cagggacaaa atggagattt accagttgtt cctgcaacac
    16621 atcattttct cgctgaaacc gggtggcaag gcggcggtcg tcgtgccgac cgggtttatc
    16681 accgcgcaat cgggcattga taaaggcatt cgcgaacatc tggtgcaaaa caagatgctg
    16741 gcgggcgtgg tttccatgcc gtcgaatatc ttcgccacca ccggtaccaa cgtgtcgatc
    16801 ctgtttattg acgccagcaa caaagagaaa gtggtgctga tcgacgcctc gaatttaggt
    16861 gaaaaggtca aagacggcaa aaaccagaaa acggtgctga cggagtgcga agaaaaacgg
    16921 atctgcgaag cgtttaacaa caagtggagc gaagaggatt tctcggtggt ggtcagctat
    16981 gacgacatcg ccgcgaaaaa ttactcgttc agcgccggac agtattttga tgtgaaaatc
    17041 gaatacaccg acatgacgcc ggaacagttt gccgcgaaga tgaaaggatt tacggagaat
    17101 ctgaataatc tctttgaaca gtcgcgcgag ttagaagtgc aaataaaaat gcagatggca
    17161 gatatcaaat atgaaattta attatctgaa aattaaagat gtatgtgatt ttgtc
//
LOCUS       DACADF010000102        17181 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.104, whole genome shotgun sequence.
ACCESSION   DACADF010000102 DACADF010000000
VERSION     DACADF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.104"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(13..1467)
                     /gene="xylB"
                     /locus_tag="ISB30_003230"
     CDS             complement(13..1467)
                     /gene="xylB"
                     /locus_tag="ISB30_003230"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAP1990832.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDDMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAARQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1538..2860)
                     /gene="xylA"
                     /locus_tag="ISB30_003231"
     CDS             complement(1538..2860)
                     /gene="xylA"
                     /locus_tag="ISB30_003231"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAP1990833.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3226..4218
                     /gene="xylF"
                     /locus_tag="ISB30_003232"
     CDS             3226..4218
                     /gene="xylF"
                     /locus_tag="ISB30_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1990834.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4296..5837
                     /gene="xylG"
                     /locus_tag="ISB30_003233"
     CDS             4296..5837
                     /gene="xylG"
                     /locus_tag="ISB30_003233"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAP1990835.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGSISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5815..6996
                     /gene="xylH"
                     /locus_tag="ISB30_003234"
     CDS             5815..6996
                     /gene="xylH"
                     /locus_tag="ISB30_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAP1990836.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7074..8252
                     /locus_tag="ISB30_003235"
     CDS             7074..8252
                     /locus_tag="ISB30_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HAP1990837.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8360..9184)
                     /locus_tag="ISB30_003236"
     CDS             complement(8360..9184)
                     /locus_tag="ISB30_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAP1990838.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9504..11534
                     /gene="malS"
                     /locus_tag="ISB30_003237"
     CDS             9504..11534
                     /gene="malS"
                     /locus_tag="ISB30_003237"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAP1990839.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWNDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKIIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALHEWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11712..12965
                     /gene="avtA"
                     /locus_tag="ISB30_003238"
     CDS             11712..12965
                     /gene="avtA"
                     /locus_tag="ISB30_003238"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAP1990840.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13004..13477)
                     /locus_tag="ISB30_003239"
     CDS             complement(13004..13477)
                     /locus_tag="ISB30_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAP1990841.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13579..14427)
                     /gene="yiaJ"
                     /locus_tag="ISB30_003240"
     CDS             complement(13579..14427)
                     /gene="yiaJ"
                     /locus_tag="ISB30_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAP1990842.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14628..15626
                     /gene="yiaK"
                     /locus_tag="ISB30_003241"
     CDS             14628..15626
                     /gene="yiaK"
                     /locus_tag="ISB30_003241"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAP1990843.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMCGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15638..16102
                     /locus_tag="ISB30_003242"
     CDS             15638..16102
                     /locus_tag="ISB30_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAP1990844.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTTDFNALDPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKIVVKVALAAL"
     gene            16125..17045
                     /locus_tag="ISB30_003243"
     CDS             16125..17045
                     /locus_tag="ISB30_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HAP1990845.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINALQANKVLALALQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
BASE COUNT         4410 a         4331 c         4208 g         4232 t
ORIGIN      
        1 aggggataac gtttacgcca ttaatggcag aagttgctga tagaggcgac ggaacgtttc
       61 tcgtcgtggc tgataagcgg cataacgctg cgcatctggt agatgcgact gttctaacgg
      121 tagttgcggc aacaactcaa tgagcgattt ctctggattc gccgcaatct gcgccagcct
      181 tgctgcgccc agtgctggcc ccacatcgcc acccgtgcgg taatccagtt gctgaccgct
      241 aatgtccgcc agcatttgac gccagtactc actacgcgcc ccgccaccaa tcaacgtaac
      301 actttgcggt ttaatgccgc aggcatgcac gacatccatg ccatctgcca gcgcataacc
      361 cacgccttcc agcactgctc gcgccagttc attggggcca tgttgatgag tcaaaccaaa
      421 gaaaacccct ttcgcctggg gattattgtg tggtgtacgc tcgccagaaa gataaggcag
      481 aaaccaaact ggctcggcac tttcatcagc ctgtcgagct gcagtgatta aagcagggac
      541 attgctcagg ccggttaatt tcgcggccca atccagacac gaagccgcac tcagcattac
      601 agacattaaa tgccaacgtt gcggtaacgc atggcaaaag ctatgtacgg cgctttctgg
      661 cttgcttaag aacccttcgc tgacagcaaa atagaccccc gacgtcccca gcgataacat
      721 tgcctgatta gcatcaacca ttcccacacc aaccgcacca gctgcattgt cgccaccgcc
      781 tgcgacaact ggcaccgccg ccatacccca cgctttcgca acttcaggta gcaaagcacc
      841 agtaatttcg ctgccttcgt ataatgcggg catctggtca cgagataagt ggcaagcctg
      901 cagcatgtcg tcactccagt cacgctttgc gacatccagc cacatggtgc cagctgcgtc
      961 agacatatcg ctggcaaact cccccgtcat acgcagacgc aagtaatctt tcggtaataa
     1021 tactttatcg atttgacgga atatctccgg ctcatgccgc tgaacccata gcaatttagg
     1081 cgcagtaaat ccgggcatca tcaggttgcc ggtaatcacc cgcgattgcg gaactcgcgc
     1141 ttccagcaaa gtgcactctt gcgcacagcg cccgtcgttc cacaaaatgg cagggcgtaa
     1201 cacccgttgc tgagcatcca gtaaggttgc accatgcatc tgtccggcaa tacctaatgc
     1261 tttaacgtct tgcagagaat gctgaacgcc cagagctttc attgcgcgat cagttgcctg
     1321 ccaccactgt tccgggtctt gttccgacca gagtggatgc gggcgcgaaa cggtcagctt
     1381 ttccgtttgc gcagcaacca cctcaccctg ctcgttgagc aaaataactt ttacgcccga
     1441 ggtgccaaga tctatcccga tatacatatc gatcgttcct taaaaaatgc ccggtatcgc
     1501 taccgataac cgggccaacg gactgcacag ttaaccgtta tttgtcgaac agataatgat
     1561 ttaccagatt ttccagttgc tcctggcgac cactctgatg caccggagac aaattatgtt
     1621 cctgagcata tttggctaaa tctgccagtg acatttggcc tttcaggatt tgctggccta
     1681 attcactatt ccagccggaa taacgctgcg cgatgcgttt atccagctcg ccatcttcaa
     1741 tcatgcgcgc tgcaattttc agcgccaacg ccatcgtatc catcgcgccg atatgaccgt
     1801 aaaacagatc atatttatca gtactttgac gacgtacttt ggcatcgaag ttcagaccac
     1861 cggtggtgaa accgcctgct ttgagaattt catacatcac cagcgcattc tcttccacac
     1921 tgttcgggaa ctggtcggtg tcccagccca gttgcgcatc gccacggttg gcgtcgacag
     1981 aaccgaacag gccaagcgca atggcggtgg ctatttcatg atggaaagag tgacctgcca
     2041 gcgtcgcgtg gttagcttca atgttcagtt taatctcttt ttccagacca aactgtttca
     2101 ggaagccata gaccgtcgca gcatcgtaat catattgatg tttggtcggt tcttgcggtt
     2161 tcggttcgat aagcaacgtg ccctggaaac cgattttatg tttatgctca accaccatct
     2221 gcataaagcg gcccagctgt tcacgctcct ggcgcaagtc ggtatttaac agcgtttcgt
     2281 aaccttcacg accaccccac aggacatagt tttcaccgcc caatttatgg gttgcttcca
     2341 tcgctgtaac aacttgcgtt gccgcccaac tgaagacttc aggatctggg ttcgtcgccg
     2401 cacccgcgcc atagcgaggg tttgtaaagc agttagcagt tccccacagc agcttcacgc
     2461 cgctctcttc ttgcttgcct gccaggacat caaccatttg cgcaaaatta ttgatgtact
     2521 cttttaacga cgcgccctca ggggaaacat ccacatcgtg gaagcaataa aatggcacat
     2581 gtaacttgtg gaaaaactca aatgcgacat ctgctttacg cttcgccaac gccagtgcct
     2641 caccaggctg ctgccacgga cgattaaacg cccccacacc aaacatatcc gccccgttcc
     2701 agcagaaggt gtgccagtag caggcggcaa aacgcaagtg ctcttccata cgcttgccca
     2761 acaccagttc gtcgggattg tagtgacgga atgctaacgg gtttgaggat tttgagcctt
     2821 cataacgaac gcgatcgagc tggtcaaaat aggcttgcat attgaactcc ataatcaggt
     2881 aatgccgcgg gtgatggatg atgtcgtaat attgggcact ccctttcagt tgctcaatta
     2941 tgttatttca cactgctatt gagataattc acaagtgtgc gctcgctcgc aaaataaaat
     3001 gggatgatga aactgggtaa ttcctcgaag agaaaaatgc aataagtaca attgcgcaac
     3061 aaaagtaaga tctcggtcat aaatcaagaa ataaaccaaa aatcgtaatc gaaagataaa
     3121 aatctgtaat tgttttcccc tgtttagttg ctaaaaattg gttacgttta tcgcggtgat
     3181 tgttacttat taaagctgtc ctctaactac agaaggccct acaccatgaa aataaagaac
     3241 attctactca ccctttgcac ctcacttctg cttaccaacg ttgcggcaca cgccaaagaa
     3301 gtcaaaatag gtatggcgat tgatgatctc cgtcttgaac gctggcaaaa agatcgagat
     3361 atttttgtga aaaaagcaga atctctcggc gcgaaagtat ttgtacagtc tgcaaatggc
     3421 aatgaagaaa cacaaatgtc gcagattgaa aacatgatta accggggtgt cgatgttctt
     3481 gtcattattc cttataacgg tcaggtatta agtaacgttg taaaagaagc caaacaagaa
     3541 ggtattaaag tattagctta cgaccgtatg attaacgatg cggatatcga tttttatatt
     3601 tctttcgata acgaaaaagt cggtgaactg caggcaaaag ccctggtcga tattgttccg
     3661 caaggtaatt acttcctgat gggcggctcg ccggtagata acaacgccaa gctgttccgc
     3721 gccgggcaaa tgaaagtatt aaaaccttat gttgattccg gaaaaattaa agtcgttggt
     3781 gaccaatggg ttgatggctg gttaccggaa aacgcattga aaattatgga aaacgcgcta
     3841 accgccaata ataacaaaat tgatgctgta gttgcctcaa acgatgccac cgcaggaggg
     3901 gcaattcagg cattaagcgc gcaaggttta tcagggaaag tagcaatttc cggccaggat
     3961 gcggatcttg caggtattaa acgtattgct gccggtacgc aaactatgac ggtgtataaa
     4021 cctattacat tgttggcaaa tactgccgca gaaattgccg ttgagttggg caatggtcag
     4081 gagccaaaag cggataccac actgaataat ggcctgaaag atgttccctc ccgcctgctg
     4141 acaccgatcg atgtgaataa aaacaacatc aaagatacgg tagttaaaga cggattccac
     4201 aaagagagcg agctgtaagc gttacgcccc ggcgcggagc gggggcgtga tttctctcca
     4261 tgccgcgtta attaattggc ttaggtggag tcgttatgcc ttatctactt gaaatgaaaa
     4321 atattaccaa aaccttcggc agtgtgaagg cgattgataa cgtcagcttg cggttgaatg
     4381 ctggcgaaat cgtctcactc tgtggggaaa atgggtcagg taaatcaacg ctgatgaaag
     4441 tgctgtgtgg tatttatccc catggttcct acgaaggcga aattattttt gcgggagaag
     4501 agattcaggc gagtcacatc cgcgataccg aacgcaaagg tatcgccatt attcaccagg
     4561 aattggcgct ggtgaaagaa ctgaccgtgc tggaaaatat cttcctgggt aacgaaataa
     4621 cccataatgg cattatggat tatgacctga tgacgctacg ctgtcagaag ctgctcgcac
     4681 aggtcagttt atccatttca cctgataccc gcgttggcga tttaggactt gggcaacaac
     4741 aactggttga aattgccaag gcacttaata aacaggtgcg tttgttaatt ctcgatgagc
     4801 cgacagcctc attaactgag caggaaacgt cggttttact ggatattatt cgcgatctac
     4861 aacagcacgg tatcgcctgt atttatattt cgcacaaact caacgaagtc aaagcgattt
     4921 ccgatacgat ttgcgttatt cgcgacggtc aacacattgg tacgcgtgat gctgctggaa
     4981 tgagtgaaga cgatattatc accatgatgg tcgggcgaga gttaaccgcg ctttacccta
     5041 atgaaccaca taccaccgga gatgaaatat tacgtattga acatctgacg gcatggcatc
     5101 cggttaatcg tcatattaaa cgagttaatg atgtctcgtt ttccctgaaa cgtggcgaaa
     5161 tactgggtat cgccggactc gttggtgccg gacgtaccga gactattcag tgcctgtttg
     5221 gtgtgtggcc cggacaatgg gaagggaaaa tttatattga tggcaaacag gtagatattc
     5281 gtaactgtca gcaagccatc gcccagggga ttgcgatggt acccgaagac agaaagcgcg
     5341 acggcatcgt tccggtaatg gcggttggta aaaatattac cctcgccgca ctcaaaaaat
     5401 ttaccggtag tattagccag cttgatgacg cggcagagca aaaatgtatt ctggaatcaa
     5461 tccagcaact caaagttaaa acgtcgtccc ccgaccttgc tattggacgt ttgagcggcg
     5521 gcaatcaaca aaaagcgatc ctcgctcgct gtctgttact taacccgcgc attctcattc
     5581 ttgatgaacc caccaggggt atcgatattg gcgcgaaata cgagatctac aaattaatta
     5641 accaactcgt ccagcagggt attgccgtta ttgtcatctc ttccgaatta cctgaagtgc
     5701 tcggccttag cgatcgtgta ctggtgatgc atgaagggaa actaaaagcc aacctgataa
     5761 atcataacct gactcaggag caggtgatgg aagccgcatt gaggagcgaa catcatgtcg
     5821 aaaagcaatc cgtctgaagt gaaattagcc gtaccgacat ccggtggctt ttccgggctg
     5881 aaatcactga atttgcaggt cttcgtgatg attgcagcta tcatcgcaat catgctgttc
     5941 tttacctgga ccaccgatgg tgcctactta agcgcccgta acgtctccaa cctgttacgc
     6001 caaaccgcaa ttaccggcat cctcgcggta ggaatggtgt tcgtcataat ttctgctgaa
     6061 atcgaccttt ccgtcggctc aatgatgggg ctgttaggtg gcgtcgcggc gatttgtgac
     6121 gtctggctag gctggccttt gccacttacc atcattgtga cgttggttct gggactgctt
     6181 ctcggagcct ggaacggatg gtgggtcgcg taccgcaaag tcccttcatt tattgtcacc
     6241 ctcgcgggca tgttggcatt tcgtggcata ctcattggca tcaccaacgg cacgactgtt
     6301 tcccccacca gcgccgcgat gtcacaaatt gggcaaagct atctacctgc cagcaccggc
     6361 ttcatcattg gcgcgcttgg cttaatggct tttgttggct ggcaatggcg cggaagaatg
     6421 cgccgtcagg ctttgggttt gcagtctccg gcctctaccg cagtagtcgg tcgccaggct
     6481 ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga atgattaccg tggtgttccc
     6541 actcctgttc tgctgctaac gttactgtta ttgggcggca tgtttatggc aacgcggacg
     6601 gcatttggac ggcgcattta tgccatcggc ggcaatctgg aagcggcacg tctctccggg
     6661 attaacgttg aacgcaccaa acttgccgtg ttcgctatta acggattaat ggtagccatc
     6721 gccggattaa tcctcagttc tcgactcggc gctggttcac cttctgcggg aaatatcgcc
     6781 gaactggacg caattgcagc atgtgtgatt ggcggcacca gtctggcagg cggcgtcggg
     6841 agcgtggccg gagcggtaat gggggcattc atcatggctt cgctggataa cggcatgagt
     6901 atgatggatg taccgacctt ctggcagtat atcgttaaag gtgcgattct gttgctggca
     6961 gtatggatgg actccgcaac caaacgccgt tcttgatttt gataaaaatt ttctcaaagc
     7021 cggtaacgta ttaccggttt tgagtttttg catgattcag caggaaaaga accatgttta
     7081 ctaaacgtca ccgcatcaca ttactgttca atgccaataa agcctatgac cggcaggtag
     7141 tagaaggcgt aggggaatat ttacaggcgt cacaatcgga atgggatatt ttcattgaag
     7201 aagatttccg cgcccgcatt gataaaatca aggactggtt aggagatggc gtcattgccg
     7261 acttcgacga caaacaaatc gagcaagcgc tggctgatgt cgacgtcccc attgttgggg
     7321 ttggtggctc gtatcacctt gccgaaagtt acccacccgt tcattacatt gccaccgata
     7381 actatgcgct ggttgaaagc gcatttttgc atttaaaaga gaaaggcgtt aaccgctttg
     7441 ctttttatgg tcttccggaa tcaagcggta aacgttgggc cactgaacgc gaatatgcat
     7501 ttcgtcagct tgtcgctgaa gaaaagtatc gcggagtggt ttatcagggg ttagaaaccg
     7561 cgccagagaa ctggcaacac gcgcaaaatc ggctggcaga ctggctacaa acgctaccac
     7621 cgcaaaccgg gattattgcc gttactgacg cccgggcgcg gcatattctg caagtatgtg
     7681 aacatctaca tattcccgta ccggaaaaat tatgcgtgat tggcatcgat aacgaagaac
     7741 tgacccgcta tctgtcgcgt gtcgcccttt cttcggtcgc tcagggcgcg cggcaaatgg
     7801 gctatcaggc ggcaaaactg ttgcatcgat tattagataa agaagaaatg ccgctacagc
     7861 ggattttggt cccaccagtt cgcgtcattg aacggcgctc aacagattac cgctcgctga
     7921 ccgatcccgc cgttattcag gctatgcatt acattcgtaa tcacgcctgt aaagggatta
     7981 aagtggatca ggtactggat gcggtgggca tatcacgctc caatcttgag aagcgtttta
     8041 aagaagaggt gggtgaaacc atccatgcca tgattcatgc cgaaaagctg gagaaagcgc
     8101 gcagtctgct gatttcaacc accttgtcga tcaatgagat atcgcaaatg tgcggttatc
     8161 catcgctgca atatttctac tctgttttta aaaaagcata tgacacgacg ccaaaagagt
     8221 atcgcgatgt aaatagcgag gtcatgttgt agcgatgatg agaattgtcg gcgtcgcttc
     8281 aggtaactca gcacaaccac ctgatgggag caaaagcctt atccgactga caagtcggat
     8341 aaggctcgag gaaatgcaat tacatatgcg ccgcgattaa ccgttggtta tcctggtaca
     8401 ttgcaaacag gtagttgtta taactcttcc ctttggtcga gtagcccttc agcttgtgaa
     8461 tcatcgctgt ggcagtcact tcctgatccg ctttacgcag ctgtgcacgc gatttacgga
     8521 acgaagagta agccgggtgc gtattgaggt ttgtaacata ggcgctcacc gattctttaa
     8581 cagaactaaa ctgtgagtac cctttcactt tacccggcgc attggtacaa cgtcctttca
     8641 tgcatttcat gccgaaaaga ttgttgttgt tgcgcgccag ctttgacgtt ccccaaccgc
     8701 tttctgctgc agccatcgtc gccaccatac tggtagggat aatgtctacg cgttcaagca
     8761 aggtattcca cgggattttt cgcgtattac cagaccactt caccttgtag cgtttggcga
     8821 tgtctttcag acgcgcacgc tcagcaggtg accattggcc ctgatactgt tttgaaatca
     8881 gccagttacg ttccgcagta atggccgcat tttggctggt aatgtaaggc attacagtcc
     8941 ggagaaacgc ctttttcctt ggtgttccgg aagggtattt tcgcaaatca ggaagtgaac
     9001 tactctttgc actattgcga gaatactctt gtttactgct tacctgttta ttactagctt
     9061 tagttaagtg ggacttttga ctcgctgttg ttgtgtgcgt cttcgctaac acctcactcg
     9121 aaaacaccag agtgagtaac ataagaatca tcgccccata tcgtcgtatg ggagtcaaaa
     9181 tcatcaggtc tcctggtcgg atttaatcat tccaacacct tatatttttt acaaatttga
     9241 gagttgaatc tcaaatcata tcaaaaatag ctgtcaagag caccccaagg aatagtccaa
     9301 atctgaaact atgtcacgag ttaacgattc agattggcgc taaatcgcag aaaatgtggg
     9361 gtttatcgca aaattcagcc gttttttgcg cgcgatcgct cacccttgct tctcatcctg
     9421 tggagttacc gctcagggat gagttttgtc tggcttatcg ctggcaaact gtctgaaatc
     9481 gcagcaataa ggactcatcc gccatgaaac tcgccgcctg ttttctgaca ctccttcctg
     9541 gcttcgccgt tgccgccagc tggacttctc cggggtttcc cgcctttagc gaacagggga
     9601 cgggaacatt tgtcagccac gcacagttgc ccaaaggtac gcgtccacta acgctaaatt
     9661 ttgaccaaca gtgctggcag cctgcggatg cgataaaact caatcagatg ctttccctgc
     9721 aaccttgtag caacacgccg cctcaatggc gattgttcag ggacggcgaa tatacgctgc
     9781 aactagacac ccgatccggt acaccaacat tgatgatttc catccagaac gccgtcgaac
     9841 cggtagcaag cctggtccgt gaatgcccga aatgggatgg attaccgctc acactggatg
     9901 tcagcgccac ttttgcggag ggagccgcag tccgggatta ttacagccag caaattgcga
     9961 tagtgaagaa cggtcaaata acgttacaac ccgctgccac cagcaacggt ttactcctgc
    10021 tggaacgggc agaaactgac acatccgccc ctttcgactg gcataacgcc acggtttact
    10081 ttgtgctgac agatcgtttc gaaaacggcg atcccagtaa tgaccagagt tacggacgtc
    10141 ataaagacgg tatggcggaa attggcactt ttcacggcgg cgatttacgc ggcctgacca
    10201 acaaactgga ttacctccag caactgggcg tcaatgcttt atggataagt gccccatttg
    10261 agcaaattca cggctgggtc ggcggcggta caaaaggtga tttcccgcat tatgcctatc
    10321 atggttatta cacacaggac tggacgaatc ttgatgccaa tatgggcagt gaagccgatc
    10381 tacggacgct ggttgatagc gcgcatcagc gcggtattcg tattctcttt gatgtcgtga
    10441 tgaaccacac tggctatgcc acgctggcgg atatgcagga gtatcagttt ggcgcgttat
    10501 atctttctgg tgacgaagtg aaaaaaacgc tgggtgaacg ctggaacgac tggaaacctg
    10561 ccgccgggca aacctggcat agctttaacg attacattaa tttcagcgac aaaacaggct
    10621 gggataaatg gtggggaaaa aactggatca gaacggatat cggcgattac gacaatcctg
    10681 gattcgacga tctcactatg tcgctggcct ttttgccgga tatcaaaacc gaatcaacta
    10741 ccgcttctgg tctgccagtg ttctataaaa acaaaacgga tacccacgct aaaatcatcg
    10801 acggctttac ccctcgcgat tacttaaccc actggttaag tcagtgggtc cgcgactatg
    10861 ggattgatgg ttttcgggtc gataccgcca aacatgttga gttgcccgcc tggcagcaac
    10921 tgaaaaccga agccagcgct gcacttcacg aatggaaaaa agctaacccc gacaaagcat
    10981 tagatgacaa acctttctgg atgaccggtg aagcctgggg ccacggcgtg atgcaaagtg
    11041 actactatcg ccacggcttc gatgcgatga tcaatttcga ttatcaggag caggcggcga
    11101 aagctgtcga ttgtctggcg cagatggata cgacctggca gcaaatggcg gagaaattgc
    11161 aggatttcaa cgtgttgagc tacctctcgt cgcatgatac ccgcctgttc cgtgaagggg
    11221 gcgacaaagc agcagagtta ttattattag caccaggcgc ggtacaaatt ttttatggcg
    11281 atgaatcctc gcgtccgttc ggtcctaccg gttctgatcc gctgcaaggt acgcgttcgg
    11341 atatgaactg gcaggatgtt agcggtaaat ctgccgccag cgtcgcgcac tggcagaaaa
    11401 tcagccagtt ccgcgcccgc catcccgcaa ttggcgcggg caaacaaacg acactttcgc
    11461 tgaagcaggg ctacggcttt gttcgtgagc atggcgacga taaagtgctg gtcatctggg
    11521 cggggcaaca gtgacttttc cggcttcccg ttcgtgagta cctcgggaag ccgccaacca
    11581 ggataaaatg tcagccctaa tcagcattgc aggataaagc cccgctcact cttcaacaga
    11641 ccgatttgca ccccagcaaa tgtagcgtta ttgttacctt ccttgctaca gagttcgaca
    11701 gatatcccgc tatgacattc tccctttttg gtgacaaatt tacccgccac tccggcatta
    11761 cgctgctgat ggaagatctg aacgacggtt tacgcacgcc tggcgcgatt atgctcggcg
    11821 gcggtaatcc ggcgcagatc ccggaaatgc aggactactt ccagacgcta ctgaccgaca
    11881 tgctggaaag tggcaaagcg actgatgcac tgtgcaatta cgacggtcca caggggaaaa
    11941 cggagctact cacactgctt gccggaatgc tgcgcgagaa gttgggctgg gatatcgaac
    12001 cacagaatat tgcactaaca aacggcagcc agagcgcgtt tttctactta tttaatctgt
    12061 ttgccggacg ccgtgccgat ggtcgggtca aaaaagtgct gttcccgctg gcaccggaat
    12121 acattggcta cgctgacgcc ggactggaag aagatctgtt tgtctctgcg cgtccgaata
    12181 ttgaactgct gccggaaggc cagtttaaat accacgtcga ttttgagcat ctacatattg
    12241 gcgaagaaac cgggatgatt tgcgtctccc ggccgacgaa tccaacaggc aatgtgatta
    12301 ctgacgaaga gttgctgaag cttgacgcgc tggcgaatca acacggcatt ccgctggtga
    12361 ttgataacgc ttatggcgtc ccgttcccgg gtatcatctt cagcgaagcg cgcccgctat
    12421 ggaatccgaa tatcgtgctg tgcatgagtc tttccaagct ggggctacct ggctcccgct
    12481 gcggcattat catcgccaat gaaaaaatca tcaccgccat caccaatatg aacggcatta
    12541 tcagcctggc acctggcggt attggtccgg cgatgatgtg tgaaatgatt aagcgtaacg
    12601 atctgctgcg cctgtctgaa acagtcatca aaccgtttta ctaccagcgt gttcaggaaa
    12661 ctatcgccat cattcgccgc tatttaccgg aagatcgctg cctgattcat aaaccggaag
    12721 gagccatttt cctctggcta tggtttaagg atttgcccat tacgaccgag cagctctacc
    12781 agcgcctgaa agcacgcggc gtgctgatgg tgccggggca caacttcttc ccgggactgg
    12841 ataaaccgtg gccgcatacg catcaatgta tgcgcatgaa ctacgtacca gagccggaga
    12901 aaattgaggc gggtgtgaag attctggcgg aagagataga aagagcctgg gctgaaagcc
    12961 attaatacgc tattccggcg ttgagaaacg ccggaaaaac gtttcaaaca ggctgctgcc
    13021 gtagccgatg ccgctgcact ttctcgacat ccatgcactg caacgccttt gtcgggcagg
    13081 cttcaacaca ggccggcccc gtcgcccgat gccagcacag atcgcactta atcgccctcg
    13141 cctttttgcg cgaagaaacg acctccatcg caccaaacgg gcaagccagc atacagcttt
    13201 tacagccaat gcaacgtgat tgttcaacga aaatatgccc atgttcgcgg cttatcgcgt
    13261 caacagggca gacattcgcg cacggtgcat cttcacactg atgacaggct actgccgtgg
    13321 tccagctgtg gtctttaatg acacgaatac gggaaataaa ctcgtctggt gacaacgcag
    13381 cgcaatcctg attctcctga tgcgacactg cgcaagccac ttcacaggta cggcaaccga
    13441 tacatttcgt cgcatccgca ataataaacc ggttcatgat cactcctttg cactaaaaca
    13501 gcagagtgcc agagagcgcg tccctactgc tttgatccaa cgaaggaaat gttaaagttt
    13561 tggcacttgt ccaaagggtt atgtaatcgc gccctggtcg tccctgacag taaatcccag
    13621 ctcattagaa atagcctgcg cggtttcacg cagtggtttc aggagatttt tctctcccac
    13681 ctgtttcaga cgtgatgtcg aaagcgaaat cgacacggcg tacggcaccc gcccatgaat
    13741 atcaaacacc ggaacagcaa tacaggagac gccgagttcg ttttcttctc tgtccatcgc
    13801 cgctccactt tcacgaatgt gcgccagttc gtcgaacatc gcgggcagct cggtaatggt
    13861 attgcgggtt aacggctgga tctcatgctg atggctttcc cagtatgact tcacgtagtc
    13921 cgggtgacca aacgccatgt agatcttgcc cattgcggaa cagtagagcg gcatatgctg
    13981 gcctatatag gcacgggttc gcagcatccc ggttgtgggt tccagcttat aaatcaaaat
    14041 agcgtgatcg tcttcgcggc tggagaagtt aatggtttca ccagtggcga tgttcagtgc
    14101 ctcaagatgc ggagcggcga tatgaatgat attcagcgaa gatagcgcct tctgcccgac
    14161 ggcaataaat ttggtggtca ggcgataact ccctgcggcg ggcgcggtgg tcacatagcc
    14221 gcaggactgt aatccctgca ataagcgatg gacggtactc ttatttaaac cagccagctc
    14281 cgaaagatgc gccaacggac aaccgtttgg atagttgctc aaaatctcaa tcagcatcaa
    14341 cccacgaaac agactctggc ttccggctgg acgctctttt tcctgcgcca tctcgttctc
    14401 tttttttccc atcacttctt tccccatttt gtcgcgtcct gatggtagcg caaagtgtgc
    14461 cgtagttcac gatctcgaca gataatttat aaccacttga tttttaagtt ttttgaaatt
    14521 catcaatcag attgctttgt taaaaagtga tcgatatatt tgaaatcaag tttcgtatat
    14581 tgaaatttta agccaaaaaa gcgatcaaaa aaacaaggaa gcctgggatg aaagtgacat
    14641 ttgagcagtt aaaagcagcc tttaatcggg tcttaatttc acgcggcgtt gacagcgaaa
    14701 cggctgacgc ctgtgcagag atgttcgccc gcaccaccga atccggcgtt tattctcacg
    14761 gcgttaatcg tttccctcgt ttcattcaac aactggaaaa cggcgatatc attcctgatg
    14821 cccaacccaa acgtataacc agcctcggcg caattgaaca gtgggacgcc cagcgttcga
    14881 tcggtaacct gacagcgaaa aagatgatgg atcgcgccat tgaactggct gccgatcacg
    14941 gtattggtct ggtggcacta cgtaatgcca accactggat gtgcggcggc agctacggct
    15001 ggcaggcggc ggaaaaaggc tacattggca tttgctggac caactccatc gccgtaatgc
    15061 caccgtgggg cgcaaaagag tgtcgcatcg gcaccaaccc gctgatcgtc gccattcctt
    15121 ccacgccgat caccatggtc gatatgtcga tgtcgatgtt ctcttacggc atgttagaag
    15181 ttaatcgcct ggctggtcgt cagctcccgg tagatggtgg ctttgatgat gagggcaatt
    15241 tgaccaaaga acctggcgtt atcgagaaaa atcgccgcat tttgccgatg ggctactgga
    15301 aaggttcggg catgtcgatt gtgctggata tgatcgctac cctcctttct gacggcgcat
    15361 cggttgccga agtcaccgag gacaacagcg acgaatacgg catttcacaa atttttattg
    15421 ccattgaagt ggacaagctt atcgacggtc ccacccgcga tgccaaactg caacgcatta
    15481 tggattacgt taccagtgcc gagcgtgctg acgaaaatca ggccattcgc ttacccggcc
    15541 acgaatttac taccctgctg gcagaaaacc gccgtaacgg tattaccgtt gatgacagcg
    15601 tgtgggcaaa aatccaggcg ttataaggag ataagccatg atttttggac atatcgcgca
    15661 gcctaatccg tgccgtttgc ccgccgccat tgaaaaggcg ctcgattttc tgcgcaccac
    15721 cgacttcaac gccctggatc cgggcgttgt cgaaatcgac ggcaagaata tttatgcgca
    15781 aattatcgat ttaaccacac gcgaagcggt ggaaaaccgt ccggaagtgc atcgtcggta
    15841 tatcgatatt cagtttctgg cgtggggtga agagaaaatt ggcattgcca ttgatacggg
    15901 aaacaataaa gtcagcgaat cactattaga gcagcgcgat attatttttt atcacgatag
    15961 tgaacatgaa tcatttatag aaatgatacc gggtagttac gctattttct tcccgcagga
    16021 tgtacatcgc ccggcatgta ataaaaatgc cactacggca atacgcaaga tcgtggttaa
    16081 agtggccctc gctgctttat aaaaaatgat tactggagca cataatgaaa acgaataatg
    16141 ccggttatat tatcggcgcg tatccctgtg caccttcttt tcaccaaaga agtgaagagg
    16201 aagagaagga tttctggcga caattatctg agactccaga cattttgggt ctggaacaac
    16261 cttgcctgga aaattttcac cctcttggcg atcaatggtt actccgtcat acgccagaga
    16321 gctggaaatt tgtcgtcacc gcagtgatgg aaaccatgcg tcgccgcagc gaaaacagcg
    16381 ggtttggtct cgcctccagt gatgaagaac agcgccaggc ctgcgtggcg tactatcgcc
    16441 acctgttcaa taaaatcaac gcattgcagg ctaataaagt gctcgcactg gcgctccagg
    16501 ctgcaccgtt agcaacgaat cctaatgtta tgcaagcgac ggacgctttt gcccgctcat
    16561 tgaaagaaat cgccagttgg gactggccgt gcaagctggt gctggaacac tgcgacgcca
    16621 tgacatcttc ttccccccga aaaggctttt tgcctttaga aaatgtgctg gaagttatta
    16681 ccgattatga catcagcatc tgtatcaact gggcgcgctc tgccattgaa gggcgcaata
    16741 caacgctgcc gctgacccac acccaaatgg cgaaacaagc cggaaaactc ggcgcgttga
    16801 tgttctctgg tacgacgcta aacggggcat acggcgaatg gcaggattta cacgcaccgt
    16861 ttgcaccatt ctgcgcagaa agcctgatga ccacagatca tgttcgcgag ttatttaatg
    16921 tagcagaatc gtcaacattg cattttgctg gcatcaagct actggaaata aatgcgacgg
    16981 ctgatgtgca ccatcgcatc gaaatattac gtaacggcat tcactctctg aatgaatctc
    17041 gttaaataga tccgcttata tatcactttg aatatctaat tagccgtttt ctgataatta
    17101 ttacgaatat taacaacatt agcgcataaa aaatattgca taaccagaag gactcgttat
    17161 gaaacggata ctcgaaggca t
//
LOCUS       DACADF010000103        16822 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.105, whole genome shotgun sequence.
ACCESSION   DACADF010000103 DACADF010000000
VERSION     DACADF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.105"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            54..896
                     /locus_tag="ISB30_003244"
     CDS             54..896
                     /locus_tag="ISB30_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadC family transcriptional regulator"
                     /protein_id="HAP1990846.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSFPESSPAEALIITDQIQKTTPEEISSTTPPVPVRKKHKGLMGLALAAAVILVGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQILAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     VQKC"
     gene            complement(955..1932)
                     /locus_tag="ISB30_003245"
     CDS             complement(955..1932)
                     /locus_tag="ISB30_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HAP1990847.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVASTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQSTALMQML
                     DKRAALSGGAVDRELQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAGQYQTRLTNALRRVGVQVHQQADGISFSTTEKQWPDIERTLSVRTDGDK
                     SAFMPLKTFAEPSQAVRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQILLQAVNGQARISSQTVRNLLG"
     gene            complement(1949..2878)
                     /gene="flgL"
                     /locus_tag="ISB30_003246"
     CDS             complement(1949..2878)
                     /gene="flgL"
                     /locus_tag="ISB30_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HAP1990848.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGSLAHVVEQMATGKEILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLVVRDDLLEAANGTN
                     TADSLASLGQDIESLTDSMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(2893..4269)
                     /gene="flgK"
                     /locus_tag="ISB30_003247"
     CDS             complement(2893..4269)
                     /gene="flgK"
                     /locus_tag="ISB30_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HAP1990849.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLTQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTPTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PDITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(4459..4758)
                     /locus_tag="ISB30_003248"
     CDS             complement(4459..4758)
                     /locus_tag="ISB30_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HAP1990850.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMLVKQLSPRHK"
     gene            complement(4758..5858)
                     /locus_tag="ISB30_003249"
     CDS             complement(4758..5858)
                     /locus_tag="ISB30_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAP1990851.1"
                     /translation="MQNWIKAAAIAVSLTLPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMTATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVSVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(5873..6538)
                     /gene="flgH"
                     /locus_tag="ISB30_003250"
     CDS             complement(5873..6538)
                     /gene="flgH"
                     /locus_tag="ISB30_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAP1990852.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPTTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(6621..7406)
                     /gene="flgG"
                     /locus_tag="ISB30_003251"
     CDS             complement(6621..7406)
                     /gene="flgG"
                     /locus_tag="ISB30_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAP1990853.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(7485..8222)
                     /locus_tag="ISB30_003252"
     CDS             complement(7485..8222)
                     /locus_tag="ISB30_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HAP1990854.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGSGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLAKNPEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(8222..9424)
                     /gene="flaE"
                     /locus_tag="ISB30_003253"
     CDS             complement(8222..9424)
                     /gene="flaE"
                     /locus_tag="ISB30_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FlaE"
                     /protein_id="HAP1990855.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFDASD
                     AAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPIDLTVDYSTCTQYGSEFSVTTN
                     AADGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(9696..10409)
                     /locus_tag="ISB30_003254"
     CDS             complement(9696..10409)
                     /locus_tag="ISB30_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping protein"
                     /protein_id="HAP1990856.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSINPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(10409..10840)
                     /gene="flgC"
                     /locus_tag="ISB30_003255"
     CDS             complement(10409..10840)
                     /gene="flgC"
                     /locus_tag="ISB30_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAP1990857.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(10843..11178)
                     /gene="flgB"
                     /locus_tag="ISB30_003256"
     CDS             complement(10843..11178)
                     /gene="flgB"
                     /locus_tag="ISB30_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838822.1"
                     /note="with FlgF and C makes up the proximal portion of
                     the flagellar basal body rod; Yersinia has 2 copies of
                     flgB and other flagellar genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAP1990858.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            11260..11997
                     /gene="flgA"
                     /locus_tag="ISB30_003257"
     CDS             11260..11997
                     /gene="flgA"
                     /locus_tag="ISB30_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAP1990859.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRRWHDYRYTFKVYIPSQIATAAPCTKTPGVTLTSPGDIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMAKSLVARDTPLTANDVELKPYNVSAQRREVLMEPNDAI
                     GFSSKHALQPGRPITKEELISPVLVERDQPVMIVYQSAGITASMPGVALKNGRKGETV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            12079..12357
                     /gene="flgM"
                     /locus_tag="ISB30_003258"
     CDS             12079..12357
                     /gene="flgM"
                     /locus_tag="ISB30_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HAP1990860.1"
                     /translation="MKITPTMPGNHPTATMAGSSQPRKSTATTATTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLASGSLQVDTEQLASDMLSFFQN"
     gene            12370..12798
                     /locus_tag="ISB30_003259"
     CDS             12370..12798
                     /locus_tag="ISB30_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HAP1990861.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRAREDL
                     LAVNDEITQHYEQLHAHSHQRHSLLKMLGVSVNRDGLAQVFAWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQAFLGSEADFLYQG"
     gene            12926..13858
                     /locus_tag="ISB30_003260"
     CDS             12926..13858
                     /locus_tag="ISB30_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HAP1990862.1"
                     /translation="MSFIECYEPEYVRNFMARHPDSPLYVRKVWKNEIRQSLMLTEPE
                     SCEAVLNDACAFDLQLTYRPVEENAAELSARDAIVNQAILSTLTLPDLTPELYLYAVG
                     IVLSRASKMPGRDGDILARLTTLPQALADHAQKGTLQAQFAQLPPVPQPARQLMTLLG
                     SCAFDWSILPESPRKTSLPLQMPLLTLHDANSKALLQQQLKVQWQTTWQQHFATAPWM
                     MRNWLIYRVYHEVIGQADGADYCPLVCDFYLIRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRESENAVLIRQQIQAERSADPLLYAFSLLSC"
     gene            13905..16343
                     /locus_tag="ISB30_003261"
     CDS             13905..16343
                     /locus_tag="ISB30_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP1990863.1"
                     /translation="MQTLIKTLVTHRVKPFVSVVTPTWNRGAFLPYLLYMYRYQDYPA
                     DRRELIILDDSPQSHQHIIDRLTNGTPESFNIRYIHHPEKLPLGKKRNMLNELARGEY
                     ILCMDDDDYYSPDKISYSIAMMQKHRALISGSDQIPIWYSHINRIFKSRSFGPHNILN
                     GTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDKDF
                     ILGASTPVNAVLTDIVSDPLLRNAYLGLHNATHYQAIHHQAIDQVVLINLDKRPDRLQ
                     QIREELTLLHIPPEKITRLAASENENGQRGRQQSHLQALRLAQQHGWQNYLLLEDDAV
                     ILKQEKHIQVLNALLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAYLV
                     NSRYYPQLAQQMNNDEHSLEDGWQPLLHTDKWLACYPSLCYQRQGFSDIEKKATDNIN
                     HYFNKLPVATKPSTLPIADTIGFFMETSFHYALYRPIITALQAQGQSCTLVINDRVFK
                     PFLDEMLATLKSIDDPQLKGMRLSEMQAHGQRVKCLVSPYHTPALNGLSTLNIRAMYG
                     LAKETWNHADWNRFYQRILCYSHYSQRALAHFGSAKVVGNPRFDAWHNGTFDRALPEN
                     IQPDSHKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLMCKLHHGTCSRPEEAASLAL
                     ARRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMTTLLDGE
                     KSYSTPESADQQIREILPVAHDVAELRYLLSEAFDWCAVQGQLAEIRHHYCDAFMDGK
                     AGERAAMVIMEALG"
BASE COUNT         3631 a         4758 c         4531 g         3902 t
ORIGIN      
        1 ttatgcaaag cactatcagg aaacatttat cccttgctaa ggtaaactca actatgagta
       61 ttattatcaa caactggcgg atggacccgt cgcttaatgc cttaatacat tgcgaaacgg
      121 gtgaaacacg tcgtctcggc gaataccatt ttattttgct ggaaacatta gcaaaaaatg
      181 ccgatgtggt tttatcgcga tcttatttat gtgccgaagt atggaagaac cgcatagtcg
      241 gcggtaacag cctgccgacg gcgatccatg cgttgcgcgt tgctatcgat gatgatggca
      301 aacaacagaa cattattaaa acaattccga agaaagggta cctgtgcaat aaagagtatg
      361 tgtcttttcc ggagtcatcg cctgcggaag cgctgattat taccgaccag atacagaaaa
      421 caacgccaga agagatatct tcgacaacac cgcctgtacc tgttagaaaa aaacataaag
      481 gactgatggg gctggcgttg gccgcggctg ttatattggt tggctcaacc gtggggtatt
      541 cacacttgaa aagtacacct gacgccccgc aactggtaaa agagtcaatt aacagtccaa
      601 gaataaaaat atttcatctt agttctggaa aggaaaataa ttccgtaccg ttactgtcac
      661 aaatcctcgc accaggaaaa gacaaactcg ataatttatt gtcagcgcat aatatgacaa
      721 tgacaaccta ttataaatat gttcgtaacc gactggaaag cgatattgtt ctgcgtaacc
      781 agtgcaacgg cagctggcaa ttaaccttta atgtggaagg ttggcagaac agcgatatta
      841 atagcgcgat gtatcagaat ttagagaagt tgttaaatac tgtgcagaaa tgctgacaag
      901 tgccaatata tgccggatgc gacgctaaac gcgtcttatc cggccaaccg ataattatcc
      961 caataaattc ctcaccgtct ggctggaaat tcgcgcctga ccgttcaccg cctgcaataa
     1021 aatctgatag ttatgattgg cgctatccag cacgccgccg aggttctccg atgcctgcac
     1081 cgcgttttcc gtttgcggga agcgtgacat gccgtcgatc aactggcggg ctttttcctg
     1141 ctgcaccgcc atctgcgcgc gctgctgatt aatgctttct aacgtctggg aaatccccgc
     1201 cccaccctgt tgcaaacttt gcgctaaacg cacggcctgc gacggttcgg caaaggtttt
     1261 tagtggcata aacgccgact tatcaccatc ggtgcgcacg cttaaggtgc gctcgatgtc
     1321 aggccactgt ttttcggtgg tggagaaact gatgccgtcc gcttgctgat gtacctgcac
     1381 gcccacgcgg cgcagggcgt tggtcaggcg cgtctggtac tgcccggcgc tgtcatcctc
     1441 agacagcatc accgccgaaa gctgggtctg ccgcccgtca gagacactga acatgcgcgt
     1501 tcccggcgtg ggattgtgta ccagattcgc cagatccggc gagtggaagg tcacccgcgc
     1561 ttcgccctgc aacactggtt gtaactcgcg gtccaccgcg ccgccggaaa gcgcggcgcg
     1621 tttgtccagc atctgcatca gcgccgtact ctgcgcctgc ccgcccttac gctggctgtg
     1681 acgatagtcg agcaactgct gttccagttg cccgagatag tgatccgcct gctgcaacgt
     1741 ggtgagctgg tcgttaagct gtacgctgta acgctgcggg cgagtggtaa tcagcggcga
     1801 ggccggatat tcactcgccg tcgccgggag tttttgccgc accgcctgag tggacgcaac
     1861 ggttgacgac gataccgcag cgctatgtgc gccgccggtg gcgagtgatg aagatgtaag
     1921 tccgacctgc atactgttcc tgaaagagtt agagaacgct gaacaaattc aactcactca
     1981 ctttcgagta agtcttgagg gtagcttcca ttgccacttc cagtccggta aaggtgatcg
     2041 acgccggacc gtaatcgaga tcctgcaatg aaccaatcaa ttcatcatta gatgtcgaga
     2101 tatcggtctg tgcatcgctc aacatcgaca tggtgttttg tgtctcgccg agcgacgcaa
     2161 tcgaggcgtt gaggtcgtcc gaggcggtgt ccaccacatc gacggcgttc tgaatatcgc
     2221 tctgcacctg cggatctgcc gggtcgacgt ccgggttttg cagttcctgc gacaaggaat
     2281 taagcgtgtt aagcacgtct aaattgctac cgaaaaaatc gctcgccgcc acgttggtgt
     2341 ccacttccac accgttcgac acggtagtct ggcgatggtc gctgttgccc tgatagctat
     2401 agctgtcggt gacgccatcg ccatcatcgt ctaccgcgac aatcggcggc tgatcgttga
     2461 tggtgccgcc aaacacgtag tgcccgtctt cgtcctgata attgagcgcc gccaccattg
     2521 aatcggtgag cgactcaata tcctggccga ggcttgccag cgaatcggcg gtattggtgc
     2581 cgttcgccgc ttcaagcagg tcgtcgcgta ccaccagcag gctgttgttg acgccatcga
     2641 gaatcgattc ctgctggctt aagcccgccg acgcggagtc gatattgctc tgatactgtt
     2701 caatcgccga ctgctggcgg tttaactggg tgatgcgcgt ggcggcgatt gggtcatccg
     2761 acggctgcaa aatttccttg ccggttgcca tctgctcgac cacatgcgcc agagagccgg
     2821 agagattatt aaaactttgc gtcatcgaga cgtaggtttg ttgggtggtt actcgcattt
     2881 tcgcgctccc gcttagctgc acatttccag caccgaatcg aaaatctcgg ccccggcgga
     2941 aatgactttc agattagctt cataaatttg ttgataggtg atcaggttca ccgcttcttc
     3001 gtccatgctg acgccgctga cgctactctg ctggttttgc gctgcggaat agacattgga
     3061 cgcggcatcg acttccgtct gattttgctg actgtaaatg ccgatattgc tgatgattga
     3121 cgagcacgcc tgcccgaccg tcacactgcc gaggttggct atctccagcg gttcggtgga
     3181 gatattgatc agcgcctgca ggttgtcgct gttgccgctt tcgtccggtg aactggaaaa
     3241 cgccagctca tcggcagtga tatccgggtt aacggtcagt gggccatcgg cattgctggc
     3301 gtcataaata aacagtggct cgccggggtt accgttgaga tcgtagccct gcgccagctg
     3361 gttgttgacc gcatcggcga actgcgacgc catgctgttg atggtgtcgg tcagcggcgt
     3421 cagcacgtcg ttttgatagt cgaacagagc gcctaacgaa ccgccagtgt cggtggtcat
     3481 ggtcgaggtg gtgccagcga aagtcagcga catggtcggt gtgccgtcgg cgttggtttc
     3541 cagcgcgatg gtcgagcttt gctgcccgct caccagcggc tgaccgtttt tcagggtgac
     3601 gttgtagttg ccctggtcgt caatattgac ctgcacgtcc atcattccgc tcagttcttc
     3661 caccatctga tcgcgggcgt cgtacagcgc ggaggcgtta tcgccgttgg cttcggcctg
     3721 agcgatttgc tggttatagc tggcgatgcc gctggtcagc gtattgattt gcgataccat
     3781 cgcctgctgc tgactgatga tttccgtgct ttgcgagtcg atgtaatcca gcgtgttgtc
     3841 gatgcgcaac gacagcgccc cggcttcgct gatcacctgc tcgcgcaggg cggaatcatc
     3901 ggggctggtg gtcgcttcgt taagggcagc gaaaaagtta tcgaagccgc cgctcagact
     3961 gctgttatcg tcgctcagta ccgcttccag ttgtgtgaga tacccttgtt gggtgctgta
     4021 atagccgtaa tcgctggcgg cataccatac ctgattaacc tgatactggt tggagacgcg
     4081 gcgaatactg tcgacctgca cgccgttacc agcgctgttc ggcgaaccac cgctggcacc
     4141 gatggtgctg atctccgcca cctggcgggt gtagcccgtg gtcatggcgt tagcggtgtt
     4201 ttgcgccgtg acgttcagct ccacctgcgc cgttgatgcg ccgctgtagc cgatgttaat
     4261 catgtccatg ttgggtcctt accggtaaat cgtgccggtt aaaagcggcg tgaagttggg
     4321 gattcttaag ttccgtaaac cgggtgccaa atgccggatg cggcgtaaac gccttatccg
     4381 gcctacatac tacatacggg cgactcgtag gcctgataag acgcgacagc gtcgcatcag
     4441 gcaccgaacg ccgaagcctc atttatgcct gggcgacaac tgcttcacaa gcatcgccgc
     4501 aatcccaatc ccgtgctgct gcgccagggt gttacacaac tcatcgtcat aaaacccctg
     4561 catcatgcgc accgaatcgc tgttaaacgg gttatctttc gaatcaaaca actcgttggt
     4621 tttacgcatc tctttcagca tgttgcgtaa aaaaatcgcc tcaaactgct ccgcggcctg
     4681 cttaatatca ttggcctgaa ccttcggccc cattaacgca tcgctgccgt caatcccgcc
     4741 gctggcgttc actttcatca gatcacctcc aggtcggcat ccagcgcgcc cgcctcatgc
     4801 agcgcctgta aaatcgacat aatgtcgtcc ggcgttgcgc ctaaactgtt cagcgcattg
     4861 accagcgttt tcaggctgct cgactccggc aaactcacca cgcccgggcg cgcatgattc
     4921 accgcgatat tgctctgcgg cgttaccgcc gtgcgcccgc ccgcaaaagc atttggctgg
     4981 ctgacgttgc tggtttcgtt aatcgagacg gtcaaactgc cgtgcgacac cgccgccgca
     5041 tgcagcgcaa cgccatcgcc catcaccacc gtgccggtac gcgagttaaa caccacgcgg
     5101 gcgcggattt tctctgcctg cacctgtaca tcatccagtt gcgacataaa tgccacccgc
     5161 gcgccggggc tggtgggcgc acgcacgctg acgttggtag agctctgcgc agtagcaatg
     5221 ccgccaaacg aggcgttaat cgccgccgca atattgttgg catctttaaa cgacggacgt
     5281 ttcaggttga gggtgatagt gtcgcccatc tgaaaatcgc tggggatctc gcgctctacc
     5341 gttgcgccgt taggaattaa cccggcggtt ggcgtattaa ccgttacgct cgaaccgctg
     5401 gccccggtgg cagtcatccc gcccaccacc acgctgccct gcgccagcgc ataaacttcg
     5461 ccatccgcgc cgtgcaactg ggtcagcaac aaagtgccgc cacgaatact tttggcatcg
     5521 ccaatcgacg aaacagtgac atcgatggtc tgcccgcgcg agtacatcgg cggcagcgtg
     5581 gcgctcaccg ctaccgcggc gacgtttttc accttcggat cgattttatt tggcagttgc
     5641 acgccaaact ggcgcagcat attggtgatg gtctggttgg tgaatttgac ctggttttta
     5701 tcaccagtac cgtcgaggcc taccaccagg ctgtagccga ccagttggtt ttcacgcacg
     5761 ccctgtacgt tgaccaacga ctccagcgat tgcgccagcg ccacgccggg cagcgtcaga
     5821 cttacagcga tcgctgcggc ctttatccag ttttgcattg cactctccgg cattaaatcg
     5881 ggaacagagg atggttaaac agccgcgtca accagcctgc ggcgttggcg tcgcttaacg
     5941 cgccgcgtcc ggcataggag atgcgggcat cggcgatccg ctgcgaggaa accgagttgt
     6001 cgttttggat gtcgtcggcg cgcaccaggc cgcttaagcg gatgtactca tcgccctggt
     6061 tgagagtcag ccatttttcg ccgcgaattt ccaggatgcc gttaggctgc accgcatgga
     6121 cggaaacggt gatctccccg cgcaggctgt tctgctgctg ggaagtggcg ctgccgtcaa
     6181 agtcgcggtt ggcgtcgata gagccggaaa gtttatcttt ggtgtgtcca aaaatcgtgg
     6241 gcgcgccgat atcgacggtg ttgcttttgc cgaagttggt tttcgcctgt ttgctcgact
     6301 gggtggactc ttcgaggatc accgtcagga tatcgcccac gcggtaagcc cgccggtcgg
     6361 cggtgagcga ccagttgtag ccggtttcaa acacgccccc ggcacgcccg tctgccggag
     6421 gcggcgcttc ggtggttggt ggcgcaaagt atgcgtcgtc ttttttgacc agaatggcct
     6481 gcgattcaca cccgctaagg agtggaagca gcgccaccag ccagagataa tttttcacgt
     6541 tctgcctgtt taaatacagt gagttatgtg ctcttgtagg tcggataaga cgcgtcagcg
     6601 tcgcatccga catttcggcc ttacagcgtc tggctgatgt actgcaacat atcgtccgca
     6661 gccgagacca ttttggcgtt catctcgtac gcccgttgca ccgtaatcat cgccaccatc
     6721 tcgttgacga tatccacgtt cgatccttcc agcgcgttgt cctgcaaggt gccaagccca
     6781 tcttcgcccg gtacgccttc ggtcggctga ccgctggcgg cggtttccag atagaggttg
     6841 tcaccctcag cggaaagccc ggccgggttg gcgaagttca ccagcgtcag ttgccctaac
     6901 tcagtcacgt cgctgtcgcc gggtaagatc gccgtcaccg tgccatcctc accaaaagca
     6961 acattagtcg cccccgccgg aacgtcgatt tccggctgca acggcaagcc ctgcgagttg
     7021 gtcagtacgc cgtcagcgtt cacctgcaag ttgccgtcgc gggtataagc gatatcgccg
     7081 ttggcctttt gcacctgcaa aaacccctgc cccataatgg cgacattcat cgcgttgtcg
     7141 gtggtttcga cgttgccttc ggtgaaggtt ttctgagtgc cgacaatgcg tacgccgcta
     7201 ccgaattgca gcccggtcgg cataatgttg ttctgatcga gcatcgcgcc cggcgcttcc
     7261 tgggtctggt aaaacagatc ctgaaacatc acccggtcac gcttgaagcc ggtggtgtta
     7321 acgttggcga tgttgttggc aatcgcgctc atttcagcat cctgcgccga gaggccggtt
     7381 ttgctgatcc atagtgctgc gttcatggtg gtaaattccg tgttattttt tggccgcggt
     7441 tgccggatgc ggcgtgaacg ccttatccgg cctacgatgt gcgattacga gccgcgcaat
     7501 aagcggttgc ccgcgtcgct gatatcttcg gcggttttca tcatcttgat ctgcgcttcg
     7561 aactggcggt tcatggcgat ggacgacatc atctcgctca ccgccgagac gttgctgccc
     7621 tcgagaaaac cgccgctgac tttgatgttt tcatcgcgct gggccggaac gccgtcggca
     7681 gtcaccagca tcccttcagg atttttcgcc aggttcgcga ccgggatatc caccagcttc
     7741 aggcggtcga tatccatcgt cgcggtcacg tcaccgtcat cgggcgtcac ggagagcgtg
     7801 ccgtcgctgc caaacgaagc aatggcgttg ggcggcagaa tgatcggccc gttatctccc
     7861 agcaccagat tgccgtcgat agtcagttgc ccctggtcat cctgctgaat gttgccgttg
     7921 cgggtataca cttcgttgcc gtttttatcc tgcacagcaa tgtaacccga accctgaatc
     7981 gccacgtcca gtgggcgttc ggttttctcc gcaacaccag tgctgtcgtt cacgccgctg
     8041 gccccttcct gcgccatata gcgcgtgtcg aagccgccgc ctttcacctt atcgttagtc
     8101 gccattgcca tatcggcgcg aaaaccgttg gtgttgacgt ttgccaggtt gttggcgctg
     8161 atctgctgct cgtacagggt ctgagaagcc ccgctcaggg cggtataaat caagcggtcc
     8221 attacattgc ctggaacagc gcgtcatcga gctgcgtgct ggtggcgata actttggtgt
     8281 tggcctgata gttacgctgg gcggtcatca ggttgactaa ctcgctggtg atatcgacgt
     8341 tagagctttc gagcacgccc gacgacagcg tgccgcaggt gccggaaccg ggaacgccaa
     8401 tcagcggggt gccggattcg ccggtttgta cccacgcggt gccgctcact gcctccaggc
     8461 cgttttcatt cgggaacgtc gccagcacca cctgcccctg caacatgcgc tcgccgttgc
     8521 tgtaggtagc gtaaactttg ccatcgtcat caacctgcac accgttctga gtggcagagg
     8581 cgtagccgtc ggcggcgttg gtggtgaccg aaaattcaga gccgtattgc gtacaggtcg
     8641 agtaatcgac ggttaaatcg atgggtgcgg cggcgtcggt ctgaaactca atggtctgcg
     8701 gcgtggttgg cgaggtcagt ttcccggtgt tcgggtcgaa ggttaacgtc gtcgccggaa
     8761 cgccggtctg ctgctgaccg tcgaaggtgt actgcacttc ccaggtgttg tcgctggttt
     8821 tggtgaagta ctggcatacc gaatgttcgt tgcctaatga gtcatacacc gtggtggtgt
     8881 agctgtcggt gtacgagctg ctgttggtcg cgtcgaacgg tacggtagtg cggtcgatag
     8941 ccgcatcgct ggcatcgaag ttggcggtaa aggtcaggct gctgctggcc tgcgccggaa
     9001 tattaccggt tttgatctgg atatcggtga ctgtaccggt ttgcagggtg cccgtgtcat
     9061 cgaccggata gccctgcaaa taagcgcccg aggcgttgac gatatagccg tttttgtcgg
     9121 tttcaaacga tccggcgcgg gtataagaaa tgttgcccgc gctgtcgcag gtaacaaaaa
     9181 agccgtcgtc gttaatcgcc agatccagcg cgttaccggt ggataccagc gaaccgccgc
     9241 gcgaaatact ttgcgcggtg cctgccacgc tgacgcccat cgcctgactt ccggcataca
     9301 tggctgaaaa ctgggtggtc atcgacttat agcctaccgt tccggcgttg gcgatgttgt
     9361 tactgatccc gtccagctgt tcgttaacgg cattcagccc cgttgcagca atttcataac
     9421 tcattgtgtc ttcctgttaa ttcagatttt ggcagcgtgc ccgttgtttt gccggatgcg
     9481 gcgtgaacgc cttatccggc ctacgaacgg gtgcaaacat aaaaatatgc gcgaactcgt
     9541 aggcctgata agacgcgtta agcgtcgcat caggcatctt gcaccgaacg ccggatgcgg
     9601 cgtgaacgcc ttatccggcc tacaaaacca ttcggattca atagtttcat gtaggcctga
     9661 taagcgaagc gcatcaggca atttggcacc ggacatcacg ccacatcact gtcggcatcg
     9721 ggtacgccaa actggctaat catggtgaac ggcacttcgc ctacaccggc aacgttaagc
     9781 accggggagc tgccgtcgag cgggatgcgc acatcctcca cttcgccgga aacttcaatc
     9841 ggcacctctt cttccccggt gttggtgacc accgagacgt cgtaatcgcc cggcgggata
     9901 ccgtaatccg ccggattaat cgagaaatcc acctgacctg gcccgacgga cgaactgcct
     9961 tccgggatca gcgacaccgt atagtcatcg cctgcggcat cggtaaagtg gagatcggcg
    10021 gtggtacagg cgtcgtcaag cgtggcgcga ccattaatgg tctggtcgct cacctgcaag
    10081 gcggtggtct gcaccataat gtcgtcaccc accaggttgc cgagcgccat cacctgaatg
    10141 ttgctcatca gcaccgcgtt ggtgttggcc tgcacgctca tctcttccat catcgccacc
    10201 tgcgccatcg aagaaagctg gtcgatatat tcggtggcgt cagtcgggtc ggtcgggtcc
    10261 tggttttgga tctcagcgac aaacagcgtc aggaaggtat cgacgggcga gctatcgctg
    10321 ctactttgcg tgtcgctggc cgccacgttg ttgctttccg ccgtggcggt cggctgcggt
    10381 gattgcgcat tcagcgccag ggtattcatc aggcttctcc cagtttcagc aaactttgct
    10441 gcatactttt cacgttgttg agcacatcga cattggtttc gaaatcgcgc gaagccgaca
    10501 tcatgtccgc catctgttcc accacgttga cgtcgggata ccagacatcc ccgttagcat
    10561 cggcgagcgg cgaatgtggt tcatagcgtt tcacagctcc gccggtttgc agcacgtcct
    10621 gcacctgcac actggcaccg tcaatggcat gacgatgtgt acccgccagc aaactgtggt
    10681 gatacaccgc cgcaaacacc gggctgcgcg ctttatatgc ctgcgcctcg ctgctggcgg
    10741 gcgcattggc attcgccagg ttactggcaa cggtattcag acgcagcgtt tgcgccgtca
    10801 tcgccgaacc ggaaatctga tagatatcag taaatgacat gcttatttcc cttcaatggc
    10861 ctctctgatg ccgctgattt gcagattcag aaaggtcaga ctgctttgat aatccatact
    10921 attttgtgaa aaccgcgctt gttcgctgtt cagttccacg gtgttgccat cttccgacgg
    10981 ctgcatcggc acacggtact ggacatccac cgccgcgtta tttgcccgtt gcatctccct
    11041 ggcaaaatca atatctttag ccttgaaatt tggcgtatcg acgttcgcca gattcgcagt
    11101 cagtagctcg gtcctctcaa gacgtaactt cactgcctgc ggatgtactc ccaatgcctg
    11161 ctgaaaactg atccccattt cctgcgtcac tcctgcttca atccgcatac gctaagcaat
    11221 cgccgtgcca ttttttaagt gactgaagag aaagggttta tgagttttca attttgcagg
    11281 cggaagtttt atttcctcct ggcgctggcc ttaccgggct acgccgccgt ccatcccgtg
    11341 cagcacagcg cgcgggaaca agtcaacgcc caggtactga atgccgccag ccaaaaaatt
    11401 gaatcgctgg cgcaacagcg gcgctggcat gactatcgct acacctttaa ggtctacatt
    11461 ccctcgcaga ttgcgaccgc cgcgccctgt actaaaacac ccggcgttac gctgacttcg
    11521 cctggggaca tcgccctcaa ccggatgaac ttcaccgtca gttgcccaca aagctggcag
    11581 atgaacgtag cggtgcgtcc ggatgtactg gtgccagtag taatggcgaa gtcactggtt
    11641 gcccgcgata cgccgctcac cgcgaatgat gtggagttga agccttacaa cgtcagtgcc
    11701 cagcgccggg aggtgttgat ggaacccaat gacgcgattg gtttcagcag taaacacgcg
    11761 ctgcaaccgg gcaggccaat caccaaagaa gaactgatat ctccggtgct ggtcgagcgc
    11821 gatcaaccgg tgatgattgt ttaccagagc gcggggatca ccgcctcaat gcccggcgta
    11881 gcactgaaaa atggtcgcaa aggggaaacg gtgaaggtgc gtaacgccag cagccagcgg
    11941 gtcatcagcg cgatggtggc ggaaagcggc gtggtgacaa cggtaagcgc ggagtaattt
    12001 gcgcgggcga ttaaagtttt gccgcctgtt aaccgcttta ccggagtaac ccacccaaaa
    12061 taccgagacg aggttacgat gaaaatcacc ccgactatgc caggcaatca cccgacagcc
    12121 acaatggcag gctccagtca gccgcgcaaa agcacggcga ccaccgccac gacgctgagc
    12181 gccgatgaca ttacccaggc gggattgcag tctgcccagc aaacgctgaa cgaccagcaa
    12241 gagagcgata tcgacagcga caaagtggca caaatgcagg cgatgctagc ctccggcagc
    12301 ctgcaagtgg acaccgagca gttagccagc gacatgctga gttttttcca gaactaagag
    12361 gccgatgaag tgagtatccg gttacaacag gtgaaggcgt tgttgcaggg aattcgtgac
    12421 gacgacgcac tgtacgattc cctgcgtgaa cttctccagc gtcagcggat ctgcatgatt
    12481 cgccgcgcca gagaggatct gctggcggta aacgacgaga taacgcagca ctatgagcag
    12541 ttgcatgcgc atagccatca gcggcatagc ctgctgaaaa tgctcggtgt tagcgtaaat
    12601 cgtgacggtc tggcgcaggt ttttgcctgg cttccggcgg tacaaaaagc ggcagcgcaa
    12661 cagttgtggc aacgccttga gcaaaaagca gaacgctgca aagcctataa cgataaaaac
    12721 ggcgagctgt taattcgcca gtatgaattt attcaggctt ttttaggcag cgaagcggat
    12781 tttctctatc agggataacg acaattcctt cccccatcgc ggaaacggat tttccgcctg
    12841 atccccaatc ctttgttttt gattaaaaaa attcccggca cgcttattgc acatcctgca
    12901 aggtaataaa gactggaaac tacctatgag ttttattgaa tgttacgaac cggaatatgt
    12961 gcgcaatttt atggcacgcc accccgattc gcctttatac gtccgcaagg tgtggaaaaa
    13021 cgagattcgc cagagcctga tgctgacaga gccagaaagc tgcgaagcgg tgctcaacga
    13081 tgcctgcgcc tttgatttac agctcaccta ccgcccggtg gaagaaaatg ccgcagaact
    13141 ctccgcgcgc gatgcgatag taaaccaggc cattttgagc acccttacgc tccccgattt
    13201 aaccccggag ctttacctgt atgccgtcgg aattgtgctc tcccgcgcca gtaaaatgcc
    13261 gggcagagat ggagatattc ttgcccgctt gacgacgctg ccacaggcgc tggccgatca
    13321 tgcacaaaaa ggcacgctac aggcacagtt cgcccagctc ccgcccgtgc cacagccggc
    13381 gcgccagttg atgacgctgc tcggcagttg tgcatttgac tggtcgattt tgccggaaag
    13441 cccgcgcaaa acttccctgc cgttgcaaat gcctttgctg acgctacatg acgccaacag
    13501 caaagccctg ttacaacagc agcttaaggt gcagtggcaa accacctggc aacagcattt
    13561 tgccaccgcg ccgtggatga tgcgtaactg gctgatttac cgcgtttacc atgaggtgat
    13621 tggacaggca gacggcgcag attattgccc gctggtgtgc gatttttatc ttatccgcac
    13681 gttaatcagt ttatggacgc tggatggttc tccgctgcga caggaggata ttttcgccct
    13741 cttcgccatg tttgaacgct ggcgagagag tgaaaatgcc gtgctgattc gccaacaaat
    13801 tcaggctgaa cgttccgcag atcccctgct ttatgcgttt tcactgctgt cttgctaacg
    13861 ctttctggcc ggttttcccg gccttatcga cttcgtaaac tatcatgcaa accctcatca
    13921 aaacactcgt cactcaccgc gtaaaaccgt ttgtcagcgt cgtcacgcca acctggaacc
    13981 gcggcgcatt cctgccgtac ctgctttata tgtaccgcta tcaggactac ccggcagacc
    14041 gccgcgaact catcatcctc gacgactccc cgcaaagcca tcaacacatc atcgaccgcc
    14101 tgaccaatgg cacgccggaa tccttcaaca tccgctacat ccatcacccg gaaaaactgc
    14161 cgctgggcaa aaaacgcaat atgctcaacg aactggcgcg cggcgaatac atcctctgca
    14221 tggacgatga cgactactac tcgccagata aaatctctta ctccatcgcc atgatgcaaa
    14281 aacaccgggc gctcatctcc ggttccgacc agatccccat ctggtacagc cacatcaacc
    14341 gcattttcaa aagccgcagc tttggcccac ataacatcct caatggcacc ttttgctatc
    14401 accgtaacta cctgaaaaag caccgttacg acgacgattg taatttaggc gaggagaaaa
    14461 gtttcaccga caatttcagc gttaacccgc tgcaactgcc cggcgagcgc acgattctgt
    14521 gtatctcgca cagccacaac acctttgata aagactttat cctcggggcc agcacgcctg
    14581 taaacgcggt gcttacggat atcgtcagcg atccgctgct acgcaacgct tatctgggtc
    14641 tgcataacgc cactcattat caggcgatcc accatcaggc gatcgatcag gtcgtattga
    14701 tcaatctgga taaacgcccg gatcgcttac agcagatccg tgaagaactg acattgctgc
    14761 atattccgcc tgagaagatc acccgccttg ccgccagtga aaatgaaaac ggtcaacggg
    14821 gccgccagca atcacacttg caggcgctgc gtctggcgca acagcacggc tggcaaaact
    14881 acctgttgct ggaagatgac gcggttattc tcaaacagga gaaacatatt caggtgctga
    14941 acgcgctgct ggcgagtctg gcgaaaattc cctggcaggt gatgatcctc ggcggcgaaa
    15001 tttcgcaggg aacgatgcta aaaagtttgc ctgggctggt gcacgcgcgg gactgccgca
    15061 aagtgtgcgc ttatctggtg aacagccgct actacccgca gctggcgcag cagatgaaca
    15121 acgatgagca ttcgctggaa gacggctggc agccgctgct gcacaccgac aaatggctgg
    15181 cctgttaccc gagcctttgt tatcagcgac agggctttag tgatatcgag aaaaaagcga
    15241 ctgataacat taaccactac ttcaataagt tacccgtagc caccaagcca tctacattac
    15301 ctatcgccga taccatcggc ttttttatgg aaacctcatt ccactacgcg ctctaccgcc
    15361 caatcatcac cgccctgcag gcgcaggggc agagctgtac gctggtgatc aatgaccgcg
    15421 tatttaaacc atttctggat gaaatgctgg cgacgctaaa aagcatcgac gatccgcagc
    15481 tcaaaggaat gcgcttatct gaaatgcagg cacacggtca gcgagtgaaa tgcctggtca
    15541 gcccctatca cacgcccgcg ctcaatggcc tttcaaccct gaacattcgc gccatgtacg
    15601 gcttagccaa agaaacgtgg aaccacgccg actggaaccg tttttatcag cgcattttgt
    15661 gttacagcca ctacagccag cgggcgctgg cacatttcgg cagcgcaaaa gtggtcggca
    15721 atccgcgctt cgacgcctgg cataacggca cgtttgaccg cgctctgccg gaaaatatcc
    15781 aacccgattc ccataaacct acggtgctct acgcccccac gtttggcgca ttaagctccc
    15841 tgccccactg ggcagaaaaa ttgggccgct taagcggtga tgtgaacctg atgtgcaaac
    15901 tgcaccacgg cacttgctcg cgcccggaag aagctgcatc gctggcactg gcgcgcaggc
    15961 atttaaaaca gcgcaccgac tctgcccgcc acacgctggc gctactggca aaggccgatt
    16021 acgtgctgac cgataacagc ggctttattt tcgatgctat tcacgttgat aagcgggtga
    16081 tcctgctcga ctttccgggc atgacaacgt tgctcgacgg tgagaaaagt tattccacgc
    16141 ccgaaagtgc cgaccagcaa attcgcgaaa tattgccggt ggctcatgat gttgcggaat
    16201 tacgttatct gctatcagag gcgtttgact ggtgtgcggt acaggggcaa ctggcggaaa
    16261 ttcgtcatca ctattgcgat gcgtttatgg atggcaaggc gggagaacgg gcggcgatgg
    16321 tgattatgga ggcgctgggg taaaccgcgc aagtgcctga tgcgcttcgc ttatcaggcc
    16381 tacgcgcccc tgcaatatag tgaatttgcg atgatttgtc ggccggaaaa ggcgtttacg
    16441 ccgcatccgg cacgaacagt gcacttatct acccttcgat ttcaaataca tcgctttatg
    16501 gctggttaat cggtaaaagt tgcacatttt gatagcgaga agagctgtct ttatgaattc
    16561 cgaaaagcgc ccttctaccc tctataacca ttctttttta cctgtattgg ccgcaacata
    16621 cctcacccac gaagtggttg tagcaattcg tgggctaaag gtccgcatta tatgaaacgg
    16681 gagggcggta gcatttctga tattacatgc ctcaattttg ctgaactatt ggctatcctg
    16741 gacctcaaaa atctggtcat aagatagatt ttttaaggga atacggagta aaggtgtgga
    16801 aaactaatta ataggtttgt ct
//
LOCUS       DACADF010000104        16785 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.106, whole genome shotgun sequence.
ACCESSION   DACADF010000104 DACADF010000000
VERSION     DACADF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.106"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            65..1297
                     /locus_tag="ISB30_003262"
     CDS             65..1297
                     /locus_tag="ISB30_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP1990864.1"
                     /translation="MYRDTRREYIVRLLRNKDECSVSDLANYFSVTKETIRTDLAWLQ
                     KRGVVSRHHGGVSLKKHLMQNALSQQEYVDMSLLIKQQQQGIGYLTSLDDKGRRMVGK
                     VCVLGSFNVDIVAKVYRFPNEGETLVARETTMGPGGKGANQALAAHRAGAQIHFACKI
                     GRDQFNHFARNHIESIGIGSFTFYESELSTTGCAVIYVNDEGENMIAMSPGANHELSD
                     NDIIQLSHFIAESDVFIVQMENNLAATQLALKCAQKMQVTTILNPAPWSSDVATLLPF
                     VDIVTPNETEASAMSGMVIDNISDAVKAAKHIYNAGQCSVIITMGKRGALIYDGQQCA
                     HIPAFSAVAVDTTGAGDAFNGALAAALAKGESLVKAAWYASAFASLAVEKEGAANMPD
                     ISLVDARIQQQNVAIQTF"
     gene            1323..2246
                     /locus_tag="ISB30_003263"
     CDS             1323..2246
                     /locus_tag="ISB30_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HAP1990865.1"
                     /translation="MKTIEGIIPVMLTPFTADNKIDYLGLKRLIDWYIENGADALFAV
                     CQSSEMQYLTLEERVELASFVVEYSQQRVPVIASGHISDDLEQQIQELNAMAATGIDA
                     LVLVTNHLDPHNEGTTVFLRTLDALLMSLPESMALGLYECPAPYRRLLTDEELLYCAQ
                     SGRFQILKDVSCDLPTVTRRVQLTQHTPMTIVNANAAIAWEAMLAGSKGFCGVFANFH
                     PDLYHWLWLEGKQHPEEAKQLAWFLSLSAVSENIGYPKNAKIFHQQRGTFDCVHCRVT
                     ADDVLKKYWGLEVVLKQIADAADFQRQQLKY"
     gene            2301..3254
                     /locus_tag="ISB30_003264"
     CDS             2301..3254
                     /locus_tag="ISB30_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HAP1990866.1"
                     /translation="MKRTLMSCAVASLLVSGSALAAWPEKPIQIIVPWGAGGNTDTVA
                     RLVAEGLQKELKVNVNVVNRTGGAGVVGHDAIAKAKADGYTLGIATVEITMMHHQGMT
                     ALTYQNYTPITRLAVNLGGVQVAANSQFENIVQMTDYIKANPGKLKASGSGLNSGWHL
                     NLIGMLDSMGLPPSAVTWVPSEGSSSALMELVSGGIDFTTSSPGEAKSMVDAKMVRNL
                     ATMAEKSAGLYKDLPVFQQATGYKYHLATWNALVVPAGTPEEVSQKLTAAMQAVFAQG
                     KLQEFATKQGFEVYPLYGKEMTTFMADEDARYGKLIAKIKG"
     gene            3303..3740
                     /locus_tag="ISB30_003265"
     CDS             3303..3740
                     /locus_tag="ISB30_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HAP1990867.1"
                     /translation="MRIIPITPLFLLIFAMFIFGWSITQYGGISQYGAGFMPCIISGL
                     LLFLAAIDVAIDIKHRHRNKVSLTFDEVKALLLVITVVLLFVLLLDILGFVICSFLLL
                     FILMKIRGVRLLHSVIVSISASLIIWFIFARVLLVAFPEGILF"
     gene            3750..5237
                     /locus_tag="ISB30_003266"
     CDS             3750..5237
                     /locus_tag="ISB30_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HAP1990868.1"
                     /translation="MDILIQALTYINLNAILAIIAATAFGLFVGAIPGLTATMAVALM
                     VPFTFFLDPIPALALMIAVGASSIYAGDIPGALLRIPGTPASAAYVADSNELVKQGKV
                     NRVLGLGLVSSVIGGVIGSLILMLAAPVLAKFALNFSSYEYTWLSLLGLTCATLVVGN
                     QPLKGMVTLCLGLLLACIGYDQISGVPRFTFGQISLLQGVNFIPAMIGLFAISGAIEY
                     YRDKISQVSGVVPQTQTGINLFKGMGQVIWSRKLNLGRSSIIGTLIGALPGAGADIAA
                     WISYAISKKFSRQQELYGHGSEEAIVDSSSSNNASLAGSWIPSLVFGIPGDSAAAIII
                     GVLYMKDMNPGPTLFLFQADKLYAVFILFLIANIALLPLATIAVSFIKRIIWIDKAIL
                     YPIILIFSIVGAFAIDNSGASVVVMLVMGVLGYWLQRKEYPVSPIILGMILGPMLEKN
                     LLSSMIKSNGEWLAFVERPVSMALAVCFFLVVALQGRNIYRSFSR"
     gene            5421..6185
                     /locus_tag="ISB30_003267"
     CDS             5421..6185
                     /locus_tag="ISB30_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAP1990869.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLVTAVLLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(6299..6997)
                     /gene="thiQ"
                     /locus_tag="ISB30_003268"
     CDS             complement(6299..6997)
                     /gene="thiQ"
                     /locus_tag="ISB30_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAP1990870.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGLLTLDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSSSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(6981..8591)
                     /gene="thiP"
                     /locus_tag="ISB30_003269"
     CDS             complement(6981..8591)
                     /gene="thiP"
                     /locus_tag="ISB30_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAP1990871.1"
                     /translation="MATRRQPLIPGWLIPGISAATLVVAVALAAFLALWWNAPQGDWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRQVLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTALIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            complement(8567..9550)
                     /gene="thiB"
                     /locus_tag="ISB30_003270"
     CDS             complement(8567..9550)
                     /gene="thiB"
                     /locus_tag="ISB30_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAP1990872.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     TEAVNVPGGWKNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(9581..9680)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(9714..11369)
                     /locus_tag="ISB30_003271"
     CDS             complement(9714..11369)
                     /locus_tag="ISB30_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAP1990873.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLTEIADEPAGGLMLKGPQGEEQEIESRLEEG
                     CYYLLFDNRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLEHLTLTFYQDHIEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            11458..11589
                     /gene="sgrT"
                     /locus_tag="ISB30_003272"
     CDS             11458..11589
                     /gene="sgrT"
                     /locus_tag="ISB30_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAP1990874.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(11696..12301)
                     /gene="leuD"
                     /locus_tag="ISB30_003273"
     CDS             complement(11696..12301)
                     /gene="leuD"
                     /locus_tag="ISB30_003273"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAP1990875.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(12312..13712)
                     /gene="leuC"
                     /locus_tag="ISB30_003274"
     CDS             complement(12312..13712)
                     /gene="leuC"
                     /locus_tag="ISB30_003274"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAP1990876.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(13715..14806)
                     /gene="leuB"
                     /locus_tag="ISB30_003275"
     CDS             complement(13715..14806)
                     /gene="leuB"
                     /locus_tag="ISB30_003275"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAP1990877.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(14806..16377)
                     /gene="leuA"
                     /locus_tag="ISB30_003276"
     CDS             complement(14806..16377)
                     /gene="leuA"
                     /locus_tag="ISB30_003276"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAP1990878.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIDKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(16469..16555)
                     /gene="leuL"
                     /locus_tag="ISB30_003277"
     CDS             complement(16469..16555)
                     /gene="leuL"
                     /locus_tag="ISB30_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAP1990879.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         3905 a         4171 c         4436 g         4273 t
ORIGIN      
        1 ttcttgataa aaatcccgat aacaatttct tttttagtga taataaaatc aaaaaggaag
       61 acttatgtat cgggataccc gtcgtgaata tattgtccgg ttacttcgca acaaggatga
      121 gtgcagtgtt agcgatttag ctaactattt ttctgttacc aaagagacga ttcggacaga
      181 tttagcgtgg ttgcaaaagc gtggtgtagt gagtcgtcat catggaggcg tctcattgaa
      241 aaaacattta atgcaaaatg cgctttcgca acaagagtat gtcgatatga gtttgcttat
      301 caagcaacaa cagcagggta ttggctattt aacctcgctt gatgataaag gacgccgaat
      361 ggtaggtaaa gtttgtgtgt taggatcatt taacgtcgat attgtggcga aagtgtatcg
      421 cttcccgaat gaaggcgaaa cgttagtggc cagggaaact accatggggc cggggggtaa
      481 aggcgcgaat caggcactag ccgctcatcg ggcaggtgcg caaatacact tcgcctgtaa
      541 gattggtcgc gatcagttta atcattttgc ccgcaatcat attgagtcca ttggtattgg
      601 ttcatttact ttttatgagt ctgaactatc taccaccggt tgtgcggtca tttatgtaaa
      661 tgatgaaggt gaaaacatga ttgctatgtc tccaggggca aatcatgaat taagtgataa
      721 cgatattatt caactttctc actttatagc cgagtccgat gtattcattg tacagatgga
      781 aaataatctt gccgctactc aactggcatt aaaatgtgct caaaaaatgc aggtgacaac
      841 gattttaaat cctgcaccgt ggtccagtga tgtcgccaca ttattacctt ttgttgatat
      901 tgtgacgcca aatgaaaccg aagcatcggc gatgtcaggt atggtaattg acaatatttc
      961 tgatgctgtg aaggctgcga agcatattta taatgccggg caatgttcag taattattac
     1021 gatgggaaaa cgaggtgcat taatttatga cgggcaacaa tgtgcgcata ttccggcatt
     1081 ttcggcagtc gctgtagata caacaggtgc gggtgatgcg tttaatggcg cgctcgccgc
     1141 cgcactggcg aagggagaat cacttgttaa agcagcctgg tatgccagcg ctttcgcttc
     1201 gctggcagta gaaaaagaag gggcagccaa tatgcctgat atctctctgg tggatgccag
     1261 aattcagcag cagaatgttg ccattcagac tttctgatgt ttcactgaac gaaggatgaa
     1321 atatgaaaac aattgaaggg attattccgg taatgctaac gccttttacg gcggataata
     1381 aaattgatta cctgggtctt aaacggctaa ttgactggta tatcgaaaac ggtgccgacg
     1441 cgttatttgc cgtgtgtcag tccagtgaaa tgcagtattt aactttagaa gagcgcgtgg
     1501 agctggcgag ctttgtggtt gaatatagcc aacaacgagt accggttatt gcgtccgggc
     1561 atatttctga tgatctggaa cagcaaattc aggagctcaa tgctatggcg gcaacgggca
     1621 ttgatgcgct ggtgttagtg acgaatcatc ttgatccaca taacgaaggt accacggtat
     1681 tcctccgtac gcttgatgcg ctattgatgt cattacccga aagtatggcg ctgggtctgt
     1741 atgaatgccc ggccccgtat cgccgcttat taaccgatga ggaactgctt tactgcgcac
     1801 aaagcgggcg ttttcaaatc ttaaaagacg tcagttgtga tctacctaca gtgacgcgac
     1861 gggtgcaatt aactcagcac acgccaatga cgattgttaa tgctaatgct gccattgcct
     1921 gggaggcgat gcttgcaggt tcgaaaggtt tttgtggcgt ctttgccaac tttcatcctg
     1981 acttgtatca ctggctttgg cttgaaggaa aacaacaccc tgaagaggca aaacaactcg
     2041 cctggttttt atcgctgagt gccgtcagtg aaaatattgg ctatccgaaa aatgccaaaa
     2101 tatttcatca gcagcgagga acatttgact gtgttcattg ccgggtaacg gctgacgatg
     2161 tgctgaaaaa atattggggg ctggaggtgg tgctaaaaca aattgctgat gccgcagatt
     2221 tccagcgtca acaactcaaa tattaatatc cgacagcact tgctgttatt aaaaaataat
     2281 taagacagga gtctacaaat atgaaacgaa ccctgatgag ttgtgccgtt gcttcactgc
     2341 tggtttcagg ttccgcgctg gctgcatggc cagaaaaacc aatccaaatc attgtcccct
     2401 ggggcgcggg aggaaatacc gatacggttg cacgcctggt ggcagaaggg ctgcaaaaag
     2461 agttgaaagt gaatgtcaac gtggtgaatc gcacgggagg tgctggggtg gttggccatg
     2521 atgccattgc gaaagctaaa gcggatggct ataccctggg aattgcgacg gtagaaatca
     2581 ccatgatgca ccatcagggg atgacggcgt taacgtatca gaactacacg ccaattacac
     2641 ggctggcagt gaatctggga ggcgtgcaag tcgcagcaaa ttcgcagttc gaaaatatcg
     2701 tccagatgac cgactacatc aaggcgaatc caggtaagtt aaaagcatcg ggtagcgggt
     2761 taaattccgg ctggcatctg aacttgatag gtatgctcga cagtatgggg cttccgcctt
     2821 ctgccgtgac ctgggtgcct tctgaaggtt ccagttctgc actgatggaa ctggtttccg
     2881 gtggtataga ttttacaacg tcatcaccag gcgaagcgaa aagtatggtc gatgccaaaa
     2941 tggtgcgtaa tttagcgacc atggcagaaa aatcagcagg gctttataaa gatcttccag
     3001 tattccaaca agcaactggc tataaatatc atttggcgac atggaatgcc ttagtggttc
     3061 cggcgggtac tcctgaagag gtctcacaaa aattaactgc cgctatgcag gcagtgtttg
     3121 cgcaggggaa attacaagaa ttcgctacca aacagggttt cgaagtttac ccgctgtatg
     3181 gcaaagagat gacgacattt atggcagatg aagatgccag atacggaaaa ttaatagcca
     3241 aaatcaaagg ttaatcagac agggcggtga atatatcgcc gccaaattac agggatagca
     3301 ttatgcgcat aataccgata acgccgttat ttctgctgat ttttgccatg tttatttttg
     3361 gctggagcat aacgcaatat ggaggcatat cgcagtatgg cgctggattt atgccttgca
     3421 tcatcagtgg attgttgttg tttttagcgg ctattgatgt agcgatagat attaaacacc
     3481 ggcaccgtaa caaagtttct ttaacttttg atgaagtgaa agcactttta ctggttatta
     3541 cagtggtact actgtttgta ttactgttag atattctcgg ctttgtgatt tgctcatttt
     3601 tgttgctttt tattttaatg aaaatacgag gtgtcagact tttgcattcc gttatcgttt
     3661 cgatatcggc atctcttatt atctggttca tttttgccag agtgttgttg gtcgcctttc
     3721 ctgaaggaat cttgttttaa ggatgcaata tggatatttt gattcaggca ctaacgtata
     3781 ttaatttgaa tgccattctg gccattattg ctgctacggc ctttgggtta tttgtggggg
     3841 ccatccctgg attgaccgca acgatggcgg ttgcactaat ggtgcctttt accttctttc
     3901 ttgatcctat tcccgccctg gcattaatga ttgccgttgg tgcgtcatcc atttatgccg
     3961 gagatatccc cggcgcactt ttgcgtatac cagggacacc tgcttcggcg gcttatgttg
     4021 ctgattcaaa tgaactggtc aagcaaggga aggtgaatcg cgtattggga cttgggctgg
     4081 tgagttcggt cattggtggc gtgattggat cacttatttt gatgttggct gcgcctgtac
     4141 tggcgaaatt tgcccttaat tttagttctt atgaatacac atggctttct cttttaggct
     4201 taacttgcgc gactctggtt gtgggtaatc aaccactgaa aggcatggtg accttatgtt
     4261 tgggattatt actggcgtgt atcggttatg atcaaatatc cggagttcca cgttttacgt
     4321 ttggtcaaat cagcctgtta caaggggtga actttatccc ggcgatgatt ggtctgttcg
     4381 cgatatccgg cgcaattgaa tattatcgtg acaaaattag ccaggtaagt ggtgttgtgc
     4441 cacaaacgca aacggggatt aatttattca agggaatggg gcaggtgatt tggtcacgaa
     4501 aattaaatct tggccgaagt agtattatcg gtacgcttat cggagcatta cccggcgctg
     4561 gagcggatat tgctgcgtgg ataagttacg ccatttcaaa aaaattctct cgccagcagg
     4621 agttatatgg gcatggatct gaggaggcaa ttgttgattc ctcgagcagt aacaacgcca
     4681 gtctggcggg gagttggatc ccttcattag ttttcggtat tccgggcgat tctgccgctg
     4741 caattatcat cggcgtgctc tatatgaagg atatgaatcc agggccgaca ttatttctgt
     4801 tccaggcaga taaactttat gccgtattta tcttgtttct gatcgccaat attgcattac
     4861 taccgttagc cacaattgcg gtcagtttca tcaaacgcat catctggatt gataaagcga
     4921 ttctgtaccc gattattctg attttcagta ttgtcggtgc atttgctatc gataactcgg
     4981 gtgcttctgt cgttgtcatg ttggtcatgg gggttcttgg atactggtta caacgtaagg
     5041 aatacccggt ttcgcccatt attctgggga tgattcttgg gccaatgttg gaaaaaaact
     5101 tattatcttc aatgattaag tctaatggcg aatggctggc gtttgttgag cgcccggtat
     5161 ctatggcact ggctgtctgt ttctttttgg ttgtcgcgtt gcaaggacgc aatatctacc
     5221 gttcatttag ccgttaatcc gttgttttca gaccatccaa gttgtggatg gtctgccttc
     5281 ccctaagtaa aatcctgcgt gactgctaaa tttatactct aaatgattcg cgttgcagga
     5341 atatattcat acaaagaata gattgacctg aataccacca ctttgcacaa tccacgacga
     5401 attcatcaac cgggcacatt atgcaagcat tgctggaaca ctttattacc caatccaccg
     5461 tgtattcatt gatggcggtg gtgttggtgg cctttctgga gtcgctggcg ctggtcggtt
     5521 tgattctgcc cggtacggtg ctgatggcgg ggctgggagc gttgattggc agcggtgagt
     5581 taagtttctg gcacgcctgg ctggcaggga tcgtcggttg cctgctgggc gactggatct
     5641 ccttctggct gggctggcgt tttaaaaagc cgttgcatcg ctggtcattt ctgaagaaga
     5701 acaaagcatt actcgataaa accgaacatg cgctgcatca gcacagcatg ttcaccattc
     5761 tggtcggtcg ttttgtcggc ccgacgcgtc cgctggtgcc aatggtggca gggatgctgg
     5821 atctgccggt ggcgaaattt attacgccaa atattatcgg ctgcctgtta tggccaccgt
     5881 tttacttcct gccggggatt ctggcgggtg cggcgatcga tattcctgcc gggatgcaga
     5941 gcggtgagtt taaatggttg ctgctggtga cggcggtatt gttgtgggtt ggtggctggc
     6001 tgtgctggcg gttatggcgc agcggtaaag cgactgaccg tttgagtcat tatttgtccc
     6061 gcggtcgttt gttgtggctg acgccgttga tttctgccat cggcgtggtg gcgctggtgg
     6121 tgttaattcg ccacccgttg atgccggtgt atatcgatat tttgcgtaaa gtggttgggg
     6181 tttagaggat agccttgtgc gggttgcctg atgcgacgct tgctgcgtct tatcaggcct
     6241 acaaaacgca ctacctgtag gtcggataag gcgttcacgc cgcatccgac agtgcatatt
     6301 aacctttaat tcccaacagc gccgaagcac tcgccttacc gctcaacaac tcatcggtct
     6361 taccctgcca ggcgatacgc ccgtcggcga ccaccaccga gcgcgtggcg atccgcgccg
     6421 catcttccac actgtgcgac accatcaaga gcgtcatttt ttgctgttgg cagctcgaac
     6481 tcaccagcgt cagcatctcc tgacgtagcg ccggatcgag tgcggaaaac ggttcatcga
     6541 gcaataaaat gggctgttcg cgtaccagac aacgcgctaa cgccacgcgc tgtcgctgac
     6601 cgccggaaag ctcgccaggt aaccgcgcca ttaaattatc aatccccatc tggtgggcaa
     6661 tcgcgtgcat tttcttctgc tgcgccgcat ttagtttcaa ccccggattt agccccagcc
     6721 cgatgttctg cgcgaccgtc aggtggctga acaggttgtt ctcctgaaac agcattgaca
     6781 ccggacgacg tgacggtggc gttgtggtgt gatctacgcc gtcgagagtc agcaaaccgc
     6841 tggctggcgt cagaaaaccg gcgatcaaat ttagcagggt gcttttaccc gcgccgcttg
     6901 gcccgaggat cgccacctgc tcgccgcgtt ccaccgttaa gctaaaacgc atcggcaaat
     6961 ggtggtaaag ccaggtgata tcagtcagtt ttaacatttc gccccggtat tttttcaatc
     7021 acggtaaaca gcagaaaaca gagcagcagc agaattaacg cggtgaccgc gccgtcctgg
     7081 ctgcgatagg agccaatttg ctgatagaga taaaacggca gggtacggaa atcatcgtta
     7141 ccgaacaatg ccaccacgcc aaaatcacca atcgacagca cacaagcaaa ggccagcgcc
     7201 tgcgccagtg gacgtttcag agcgcgcagc tccaccactt ttaagcgtga ccagccttca
     7261 atgcccagcg actgacacaa catgctgtag cgggcggtga tatcgcgcat cgggttttcc
     7321 agtactttca gcgcataagg gattgccatt aacgcattgg tgaaaatcac aatgccgtca
     7381 gcagattgtg gcaggccgat agtgttgttg agcagtaaaa agaagcccgt agccagtaca
     7441 atccccggca tggcgaggat caacatgccg ctcatctcca gcgcctgacc cgccagcatt
     7501 ttctgccgtg cccgaagttc gcgactgctc catagcagca tcatggtcag cactacgcac
     7561 aatacgcctg ccgccagcgc aatacgcaac gaggtccaca gcgcctgcca cagcaccggt
     7621 tgtgccagca cttccggcaa ctggcgattt accccatcga cgatcaccgc cagtaacggt
     7681 ggcagcaaca gcagcagcgc cagcacaatt aacgccgtgt cgcaaatgcg actatgcaga
     7741 cgatcgtccg ggtcgcgcca gccttgcagc agtgtggtac cgggcgcaat ggccttactc
     7801 aatcgctgac tcaacagcac cagcccgagg cagcacacca tctggatcag cgccagcatt
     7861 gccgcgcggg caggatcgta gtcgtaactc agcgcctgat agattgccag ctcgatagtg
     7921 gtcgcctgcg gaccgccgcc cagcgatagc acggtggcga agctggcgaa acagagcata
     7981 aagataagcg cagcaaccgg cgggatttgt cgccgtaacc acggccattc gacgaagcgg
     8041 aagaagtgcc agccgcgcat cccaagttgt gcggcaagct gacgttgttc gccgggaatg
     8101 ttttccagtg cctggagtaa taaacggcta gccatcggca gattaaaaaa cacatgggcc
     8161 agcaaaatgc cttgcaggcc gtagggcgaa aaggtccact ccagaccaag tgattgccag
     8221 agcgaggcca gccagccctg gcgaccatag acgctaagaa tgccgaaaac ggcgaccagc
     8281 accgggagga tcaaggtcat tgcgcacaga cgcaacagca cctgtcgccc aggaaagcgc
     8341 ctgcgataga gcgcgcgggc gaggaatatt gcgggtacga cagagagcag cgccgagaga
     8401 aacgcctgcc agaaggagaa gcgcaccaca tgccacagat agctgtcctg ccagactgcc
     8461 acccagtcac cctgcggcgc gttccaccac agggcgagaa acgccgccag cgcaaccgct
     8521 accaccagtg tggcggcgct tattcctgga attaaccagc cgggaattaa cggctgacgg
     8581 cgcgttgcca ttcgctaatc catgcctgac gttgtgccgc cacttcggcg ggcgtgaact
     8641 ctagcgtggt ggctggtttg gtcaattgtt caaatcctgc aggcagagtg acgtttgcta
     8701 ccggatacat ccagttgccg gttgggatcg cattctgaaa agccggagaa accataaact
     8761 ggaggaattt ttgcgccagc tctggctgct tgctggcagc ggtgcgggcg gcaacttcca
     8821 cctgcagata gtgaccttcg ctgaagttcg cggcggcgta gttatctttc ttctcttcga
     8881 gaatgtgata agccggagag gtggtgtaac tcagtaccag atcgctttcg ccttttaaaa
     8941 acaggccgta ggcttcgctc cagcctttag tgaccgtgac agttttcttc gccagtttct
     9001 gccaggcttg cggggcgtta tcgccataga ctttttgcat ccatagcaac agacccagac
     9061 ctggtgtact ggtacgcgga tcttcataaa tcacccgcca gttttgatcg ctctcaacca
     9121 gttctttcag gctttgcggc ggatttttca gtttgttctt gtcatagacg aaggcgaaat
     9181 agccatagtc aaacggcaca aaggtgtcat ttttccagcc gccgggaacg tttacagcct
     9241 ctgtcgcgac gccgcttttg gcaaacaacc cggttttgct ggcggcgtcc agcaggttgt
     9301 tatccagccc cagcaccaca tcggctttgc tgtttttgcc ttccatccgt agacggttga
     9361 gcagcgaaac gccatcttcc agcgccacca gtttcagttc gcaattacag tctgcttcaa
     9421 aggctttttt gactaccgga ccaggtcccc agtcagcggc aaaggaatcg taggtataaa
     9481 cagtcagaac aggtttagcg aaaacaggcg ctgtgcacag caacagcagg ggaagacatt
     9541 tttttaacac tttgcacctc acaaaagagt ggcaaaggac ttgagaagga gcctcaaatc
     9601 ccttcgccgg cgttatccgg atcaggttcg acgggtattt tctcagcgca cgcggctgcg
     9661 tggcaccccg ttgagaacgg cgttagtgta gtgattttgt tatcaaccag caatcatgga
     9721 tccggtggtg caaaccacgc tgatttaaaa tcgaaccagc cgagggtgtt catgcgcagg
     9781 ccgcgcatac tgcgttgccc ctgaatgatc agccagtggt ggataagtgg caccattgct
     9841 ttgctggcga ccagttgctg gcaccagttc gccaggttca tttcgccatt gcgccagcgg
     9901 gcggcgtcgg cttgccagtc gatcggaata cagtgttgga gtagcggcac ctcgcacaag
     9961 tgcgcgaaca gcgaaaaatc cagcggcagc gtaaagttgg cgctgttaag ccagatatcg
    10021 ctctcgatct ctccttcatg ccactgatcg tagctgatct ctttgatttc cagcgtgacc
    10081 tggtgactcg ccagaatctg ctgcatgatc ccggcaatca ctcgatgttc aatatgatcc
    10141 tgataaaagg tcagggtgag atgttccagc ccggcgggtt tttcgcaatg tgtcgggcgg
    10201 gcatggtgcc agcgggggag cagtccataa gccgggaacc agagttgctg gtattgttcc
    10261 tcggcgaaat agaccagatt cgtcggagaa agcacatagc ttacccagtc cctgacttgc
    10321 tgattcgccc cgcggtgggt gcggttgtcg aacagtaaat aatagcaacc ttcctccagg
    10381 cggctttcaa tctcttgttc ctcgccctgc ggccctttca gcatcagccc tccggctggc
    10441 tcgtcggcaa tttccgttag aacccagacg ttaacttcgt cgattaatgc ccggtaaccg
    10501 aagaagtcat cgaatgcctg aattttcagt tgattggtgc tgttgcggat caccgcatac
    10561 ggaccggtgc cgatgggatg gctggcaaag ttactgaggg tttcccattc gcgcggcagg
    10621 atcattgccg gaacttgccc cagcagtaac ggcaaccagc ggtccggttg cgtgagatgg
    10681 atatccagcg tccagggcgt cggcgacaca atgtcagcaa tatgcgaata gagcggaagc
    10741 gtattgattc gttttaaaga ggcgatcaca tcgtccattt ccagttcacg accatggtga
    10801 aaatggactc ctggacgcaa aaagaaacgc cagtgaagcg gtgatatttg ctgccagtgg
    10861 tgtgcgatgt ctgcttccag ttccccattt tcctcattta tgcgcgttag cgaactgaag
    10921 atttgccggg cgatatgggt ttcagaacgg cgcaatgcgc tgccaggtag cagattacgc
    10981 aacggacgat agtagagcac gcgcaggatg tgccgcccct ggcggaagct gcggcccaga
    11041 tgagaaacca gcatttgccg cacagtcgct ttgtcgccaa ccaactgcac tagttggtcg
    11101 atacgatcct gctccagcag gtcttccgct cgctgttgct gaagcgccag cccggtatag
    11161 aggaatgtca gacgcgagcg tttaccgcgc ccgacttccg cttcccacgt cagccagccg
    11221 cgatcctgca tggtgttgag cagggtgcgc atatgacgac gcgagcaact cagtaacgct
    11281 gccagttcgt tgagcgtggt ctcctgagat ttaccctcgc agcattgcca caggcggatg
    11341 aactgttgtt gcagacgagc agatggcata aaaggggaac tcctgcgcaa aagacagcaa
    11401 ttttattttc cctatattaa gtcaataatt cctaacgatg aagcaaggag gtgccccatg
    11461 cgtcagtttt atcagcacta ttttaccgcg acagcgaagt tgtgctggtt gcgttggtta
    11521 agcgtcccac aacgattaac catgcttgaa gaactgatgc aatgggaggg gagtcattct
    11581 gactactgat ttgcggacat catgtgtgac tgagtattgg tgttaatcgc cacgccagca
    11641 gtgattatct gctggctttt ttcttttggc gattgagtgc cggacggtgt acaaactaac
    11701 gcataaacgc cggttgcttc tcttcataag acgcaatagc atcgtcgtgc tgcagagtca
    11761 ggccgatgct gtccagaccg ttcatcatgc agtggcggcg gaaggcatcg atggtaaagc
    11821 gataagtttt ctctcccgct ttcacctctt gcgcttccag gtctacatca aaatgaatcc
    11881 ccggattggc tttcaccagc gcaaacagct catccacttc tgcatcgctt aatttcaccg
    11941 gcagcagctg gttgttaaag ctattgccgt agaagatgtc agcaaaactc ggcgcaatca
    12001 ccaccttaaa accgtaatcg gtcaatgccc acggcgcgtg ctcacgcgag gaaccgcagc
    12061 cgaagttttc tcgtgccagc aaaatggaag cgccctgata ctgcgggaag ttcagcacga
    12121 agtccgggtt tggctgttgg cctttttcat ccagaaaacg ccagtcgtta aacagatgcg
    12181 cgccaaaacc cgtacgggtc actttctgca aaaactgttt cgggatgatt gcatcggtat
    12241 cgacattggc ggcatccaac ggaaccacca ggcctgtgtg tttgataaat ttctctgcca
    12301 tggtgtgctc cttatttaat gttacgaatg tcggcgaaat gtccggtcac agccgcggcg
    12361 gcagccattg ccgggctgac cagatgcgtg cgcccgccgc gcccctggcg gccttcaaag
    12421 ttacggttgc tggtggaggc gcaacgttcg cccggattca gacggtcgtt attcatcgcc
    12481 agacacattg agcaacctgg caagcgccat tcaaaaccag cttcgataaa aattttgtcc
    12541 agaccttccg cttccgcctg ggctttaacc ggaccggagc cgggaaccac cagcgcttgt
    12601 acgcctggcg cgactttccg gcctttggcg atttccgctg ccgcgcgtaa atcttcaata
    12661 cgcgagttag tacaggaacc aataaacact ttgtcgatag ccacttctgt cagcggaata
    12721 cccggtttca gccccatata ggccagcgct ttttctgccg acgcgcgttc aaccggatcg
    12781 gcaaacgaag ccggatcggg aatattgtcg ttcacagaaa tcacctggcc tggattggtg
    12841 ccccaggtga cctgcggcga aatctcttct gcttgcagag tgacaacggt atcgaaagtt
    12901 gctccttcgt cggtttgcag ggttttccag taggcaacgg cgtcgtcgaa atctttacct
    12961 ttcggcgcat gcagacggcc tttaacatag ttaaaggtgg tttcgtccgg cgcaaccaga
    13021 ccggcttttg cgcccatttc gattgccata ttgcacaggg tcatacgacc ttccatgctt
    13081 aaatcacgga ttgcttcgcc gcaaaattcc accacatgcc cggtaccgcc tgcgctgccg
    13141 gttttaccaa taattgccag cacgatatct tttgcggtaa tgcccggcgc ggctttgccc
    13201 tggacttcaa ttttcatggt cttcgcccgg ccctgtttca gggtttgcgt tgccagtacg
    13261 tgttcaactt cggaagtgcc gataccaaag gccagtgcgc caaacgcgcc gtgggtggcg
    13321 gtatgtgagt cgccgcagac aatggtcatc cccggcaagg tcacaccctg ttccggcccc
    13381 attacgtgga cgatcccctg atacgggtga ttcaggtcat acagctcgac gccaaattct
    13441 ttgcagtttt tgatcagctc ctgcatctgg atgcgcgcca tttcaccgca ggcattaatg
    13501 tctttcgtct gggtagagac gttgtgatcc atggtggcga aggttttgcc cggctgacgt
    13561 accgggcgac cgtgggcgcg cagaccatcg aacgcctgcg gtgaggtcac ttcatgcacc
    13621 aggtggcggt cgatatataa cagcggggtt tcgttttcgg cttcgtacac tacgtgagcg
    13681 tcgaataatt tttcgtataa cgtcttagcc atgattacac cccttctgcc acatagcggg
    13741 caatgatatc gcccatttca tcggtactaa cggcggcagc gccacgggct aaatccccgg
    13801 tgcgaatgcc ttcttctaat gcgcggttaa tggcgcgttc aatggcggaa gccgcatcat
    13861 cggcatccag gctgtaacgc agcagcagcg ccagcgacag aatttgtgca atcgggttgg
    13921 cgatattttt acctgcgata tcgggtgccg agccgcccgc cggttcatac agaccaaaac
    13981 cttgctcgtt caggctggcg gaaggcaaca tccccattga gccagtgatc attgcgcact
    14041 catcagacag aatgtcgcca aacaggttgg agcacagcaa gacgtcaaac tgtgatggat
    14101 ctttaatcag ctgcatggtg gcgttgtcga tgtacatatg cgccagttcg acgtccgggt
    14161 attccgtggc gatctcgtta acgatctccc gccataaaat agaggattgc agcacgttgg
    14221 ctttgtcgat tgaggtgact ttgtggcgac gcttgcgggc agattcaaat gcgatgcggg
    14281 caatacgctc gatctcaaaa cggtggtaca cctcggtatc aaacgctttt tcatattgtc
    14341 cgctaccttc gcggcctttt ggctgaccga aatagatgcc gccggtcagt tcgcgcacac
    14401 acaggatgtc aaagccgttt gcggcaatgt cagcacgcag cggacagaat gcttccagcc
    14461 cctgatacag ttttgccgga cgcagattgc tgaataattt gaagtgctta cgcaaaggca
    14521 gcagtgcgcc gcgttctggt tgctggtctg gcggcaaatg ttcccacttc ggaccaccta
    14581 ccgagccaaa cagcacggca tcggcttgct cacaaccttc aaccgtcgca ggcggcagcg
    14641 gctgcccgtg attatcaatg gctgcgccgc ctacatcgta atggctggtg gtgatacgca
    14701 tcgcaaatcg gttgcgcacg gcatccagca ctttcagcgc ctgggtcatc acttccggac
    14761 caataccgtc ccccggcaat acggcaatat ggtaattctt cgacatcaca cggtttcctt
    14821 gttgttttcg ttgtgttgag ctttgcgttg caactctttt tcgacttctg cggcacgcca
    14881 gatattgttc agtacgtgca ccatggcttt ggcggaggac tcgacaatat cggtggccag
    14941 gccgacgccg tggaagcggc gaccgttgta gttggcgaca atatccacct gacccagcgc
    15001 atctttaccg tgacctttgg cggtcaggct gtatttcacc agttcgacgt tatagtcagt
    15061 gatgcggttt atcgcctggt agacggcatc aaccgggccg ttgccgttag cggcttctgc
    15121 tttaacttct tcgccacagg ccagtttgac ggcggcagtg gcaatatcgt tagaacctga
    15181 ctgcacgctg aagtaatcca gacggaaatg ctccggctct tcttgctgct tatcgatgaa
    15241 ggccagcgcc tccagatcgt aatcaaacac ctggcctttt ttgtccgcca gcttcaggaa
    15301 agcgtcgtac aggttgtcta aattatattc actttcttta tatcccatct catccatgcg
    15361 atgtttcacc gccgcacgcc ccgaacgaga ggtcagattc agctggattt ggttcagacc
    15421 aatagattct ggtgtcatga tttcgtagtt ttcgcggttt ttcagtacac catcctggtg
    15481 gataccggag gagtgtgcga atgcgccgct gccaacaatg gctttgtttg ccgggatcgg
    15541 catattacaa atctggctaa ctaactggct ggtgcgccat atctcctggt gattgatggc
    15601 ggtgtggacg ttgagaatat ccttacgaac tttgatcgcc atgatgactt cttcaaggga
    15661 acagttaccg gcacgctcgc cgatcccgtt cattgcgcct tctacctgcc gtgctccggc
    15721 atgtaccgcc gccagtgagt tgccgaccgc caggcccaaa tcgtcgtggg tatgtacgga
    15781 aataatggct ttgtcgatgt tgggtacgcg ttcatacagg ccgctgatga ttccggcgaa
    15841 ctcaaatggc atggtgtagc ccacggtgtc cggaatgttg atggtggtgg caccggcatt
    15901 aatcgccgct tcgaccactc gcgccagatc ggcaattggg gtgcgtccgg catcttcgca
    15961 agaaaattca acatcatcgg tgtaattacg ggcgcgtttc accatataga tagcgcgttc
    16021 gatcacttcg tccagcgtgc tgcgcagctt ggtggcgatg tgcattggcg aagtggcaat
    16081 aaaggtatga atacggaagg cttcggcgac tttcagggat tcggctgcca cgtcgatatc
    16141 tttctccacg cagcgagcta acgcgcatac acggctgttt ttaacctggc gagcgatggt
    16201 ctgcactgat tcaaaatcac ccggcgaaga gacggggaaa ccgacttcca tcacgtcaac
    16261 acccatacgc tcaagggcca gcgcaatttg cagtttttct ttcacactca agcttgcctg
    16321 taacgcctgt tcaccgtcgc gcaatgtggt atcgaaaata atgacttgct ggctcatggt
    16381 ttgggtcctt gtctcttttt gagcgcctcg cttcgggcat aaaaaaaccc gcgcaatggc
    16441 gcgggttttt gtttgactgc gtgctggctt aatgctggat gccgctcact cgtctaccgc
    16501 gcaaagaaga tgcgtttagt agtagtagac cgataaagcg aacgatgtga gtcattaaat
    16561 cagctccaga tgaatgcgat atgcttttag agttactgga tacaaaaacg gatgtcaacc
    16621 ctgaagcaat aaaaacgtca cgccattgtg agttcggcat ccgtaaaatt agctaattgt
    16681 gctgcggtgg ttaaaacaag cgatattaat ttctgcttaa ccgccgatgc ttttcatcgg
    16741 ttgacatatt tcagaataat tttttgcatc taatcaacga gggaa
//
LOCUS       DACADF010000105        16431 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.107, whole genome shotgun sequence.
ACCESSION   DACADF010000105 DACADF010000000
VERSION     DACADF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.107"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            141..350
                     /gene="mokC"
                     /locus_tag="ISB30_003278"
     CDS             141..350
                     /gene="mokC"
                     /locus_tag="ISB30_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAP1990880.1"
                     /translation="MQNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            complement(454..1584)
                     /gene="dnaJ"
                     /locus_tag="ISB30_003279"
     CDS             complement(454..1584)
                     /gene="dnaJ"
                     /locus_tag="ISB30_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP1990881.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1673..3589)
                     /gene="dnaK"
                     /locus_tag="ISB30_003280"
     CDS             complement(1673..3589)
                     /gene="dnaK"
                     /locus_tag="ISB30_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP1990882.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3960..4364
                     /locus_tag="ISB30_003281"
     CDS             3960..4364
                     /locus_tag="ISB30_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAP1990883.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4390..5103
                     /gene="msyB"
                     /locus_tag="ISB30_003282"
     CDS             4390..5103
                     /gene="msyB"
                     /locus_tag="ISB30_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAP1990884.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5252..5818
                     /gene="satP"
                     /locus_tag="ISB30_003283"
     CDS             5252..5818
                     /gene="satP"
                     /locus_tag="ISB30_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAP1990885.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5853..6440)
                     /gene="mog"
                     /locus_tag="ISB30_003284"
     CDS             complement(5853..6440)
                     /gene="mog"
                     /locus_tag="ISB30_003284"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAP1990886.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(6555..7508)
                     /gene="tal"
                     /locus_tag="ISB30_003285"
     CDS             complement(6555..7508)
                     /gene="tal"
                     /locus_tag="ISB30_003285"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAP1990887.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            7787..9217
                     /locus_tag="ISB30_003286"
     CDS             7787..9217
                     /locus_tag="ISB30_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAP1990888.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFAMLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            9287..10063
                     /gene="yaaA"
                     /locus_tag="ISB30_003287"
     CDS             9287..10063
                     /gene="yaaA"
                     /locus_tag="ISB30_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAP1990889.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10103..10399)
                     /locus_tag="ISB30_003288"
     CDS             complement(10103..10399)
                     /locus_tag="ISB30_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAP1990890.1"
                     /translation="MKKMQSIVLALSLVLFAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(10613..11899)
                     /gene="thrC"
                     /locus_tag="ISB30_003289"
     CDS             complement(10613..11899)
                     /gene="thrC"
                     /locus_tag="ISB30_003289"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAP1990891.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHGGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11900..12832)
                     /gene="thrB"
                     /locus_tag="ISB30_003290"
     CDS             complement(11900..12832)
                     /gene="thrB"
                     /locus_tag="ISB30_003290"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAP1990892.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICQLDTAGARVLEN"
     gene            complement(12834..15296)
                     /gene="thrA"
                     /locus_tag="ISB30_003291"
     CDS             complement(12834..15296)
                     /gene="thrA"
                     /locus_tag="ISB30_003291"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAP1990893.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(15378..15443)
                     /gene="thrL"
                     /locus_tag="ISB30_003292"
     CDS             complement(15378..15443)
                     /gene="thrL"
                     /locus_tag="ISB30_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAP1990894.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15657..16343)
                     /locus_tag="ISB30_003293"
     CDS             complement(15657..16343)
                     /locus_tag="ISB30_003293"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAP1990895.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAYLEPAA
                     RWVAHGSGDIIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3836 a         4377 c         4053 g         4165 t
ORIGIN      
        1 agaattgata aaacagaagt gaattaaaag cgcagtataa tagcggcggg tgcttgaggc
       61 tgtctgtctc aggcattggc tgaacggcag atagagaaaa gccccgagtg atattttacc
      121 atcaacccga ggccccctat atgcagaaca catgtagagt gcctcttact gaccgaaagg
      181 tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg atcgtcatct
      241 gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag gttcacatcc
      301 gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa gagcaacggc
      361 ggggagtgat ccccgccact ttacaggtgc tcgcatatct tcaacgcacc ctttttttta
      421 cctggcctgc ccacgggcag gcttttgggg aggttagcga gtcaggtcgt cgaaaaactt
      481 cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcgccgg ttgggccacc
      541 aaagctttct tgcagctctt gcagcagctg tttctgcttc tcgttcaggc ctaccggtgt
      601 ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgccg ccgcggacag acttgacgcc
      661 tttaccacgc atacggaaca gcttaccggt ctgggtttcg ccaggaactt tcagtttaac
      721 gcgaccatca agggtcggca cttcgatttc gccgcccagc gccgccatag cgaagttgat
      781 cgggacttcg caatacaggt tgttgccttc acgctcgaaa atcgggtgct gtttaacctg
      841 aacctgaacg tacaggtcgc ctgccggtgc gccgtgttcg cccgcttcac cttcgcccgc
      901 aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca gcgttttgct
      961 gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca gcgtaccgcg
     1021 gccctgacag tgtggacagg tctgctgcac ggcaaagaaa ccctggcgca tctgcacctg
     1081 gccagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg caccgctacc
     1141 gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca cgccacgtac
     1201 agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg ccgcacgttg
     1261 acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat cgctgaagtc
     1321 tgcgccgccg ccaaaaccgc cgccgcccat gccgccttgc tcaaacgcag catgaccata
     1381 ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt ccttgatctc
     1441 tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt atttcatggc
     1501 caggcgtttg taggcctttt tgatttcacg ctcttccgct gttttggaaa cgcctaaaat
     1561 ctcgtaataa tcttgcttag ccatcttttt tgatttgccc ctagatgaat gcacgggcgg
     1621 agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc gattattttt
     1681 tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg ttgtttgcag
     1741 aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt tccatcagtt
     1801 tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg tcttcacctt
     1861 tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg tcagccggca
     1921 gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg tcgccctggt
     1981 tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct tctgcgtcgc
     2041 gtaccatttt ctggatttca tcttcgttca gaccagaaga agccttgata gtgatcttct
     2101 gctctttacc gctgtttttg tctttcgcgg aaacgtgcag gataccgtca gcatcgatat
     2161 cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca tccaggttga
     2221 actgacccag agatttgtta tcagccgcac gtttacgttc accctgcagc acatggatgg
     2281 ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg gtcgggatag
     2341 tggtgttttt cgcgatcagc gtggtcatca caccgcccat ggtttcgata cccagagaca
     2401 gcggggtaac gtccagcagc agtacgtctt ttacgtcacc agtcagaaca ccaccctgaa
     2461 cagcagcacc gatggctaca gcttcgtccg ggttaacatc tttacgcggc tctttaccaa
     2521 agaattcagc aactttcttc tgaaccattg gcatacgagt ctgaccacca acgaggataa
     2581 cgtcgtcgat atcagatacg gacaggccag cgtcctgcag tgcaactttc agcggctcaa
     2641 tggaacggtt taccagatct tcaaccaggc tttccagttt cgcacgagtc actttgatgt
     2701 tcatgtgttt cggaccggtc gcgtctgcag tgatgtacgg caggttaacg tcggtctgct
     2761 gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc tgcattgcca
     2821 gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc agatagttga
     2881 tcagacggct gtcgaagtct tcaccaccca ggtgggtatc accgttggtt gccagaactt
     2941 cgaaggtttt ttcgccgtca acttcgtcga tttcgataat ggaaatatcg aaagtaccac
     3001 cacccaggtc ataaaccgcg atagtacggt tgccagtacc tttgtccaga ccgtaagcca
     3061 gcgcagctgc ggtcggttcg ttgatgatac gttttacttc cagaccagcg atacggcctg
     3121 cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc cggtacggta ataacagctt
     3181 cagttaccgg ttcacccagg taatcttcag cggttttctt catttttttc agcacttcag
     3241 cagagatctg cggcggtgcc attttctggc ctttaacttc gacccatgcg tcgccgttat
     3301 cagcagcaat aattttgaac ggcatgatgg aaacatcacg ctgtacttct tcgtcctgga
     3361 agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc gtcactgcct
     3421 gacgtttagc cggctgacca accagagttt caccatcctg ggtataggca atgatagaag
     3481 gcgtggtgcg atcgccttcg gcgttctcca gtacacgagg agtggtgcca tccataatcg
     3541 ctacacaaga gttggtagta cccaggtcga taccaattat tttacccatc taaacgtctc
     3601 cactatatat tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa cgtctggttt
     3661 caactgccca atttcatttt tttgcagact cactcactgc ggttgactac taaatggggt
     3721 cgtaacccac gtcatcaagg gggagatgca aaaatttttt gtgcttttgc gcagaaattt
     3781 cgcgatatgg caggacaaga cgtgaaactg acagcaattt tattcatata aagaatgaat
     3841 cgcagcaagg cattcatcaa aaaattgtaa taaaaagaaa atattacgtg cctgaaactt
     3901 ctctttatca gcggtaaact agtttcatcc ccttacccct ccccaccaag agaatcctta
     3961 tgaaatccgt tattacgatt tccgccagcc tggcgattag cctgatgctg tgctgcacgg
     4021 cgcaggcaaa cgaccataaa atcctcggcg tcattgcaat gccgcgaaac gaaaccaacg
     4081 atctggcgct gaaacttcct gtttgtcgca ttgttaaacg catacaactc tcagccgacc
     4141 atggcgattt gcagttaagc ggtgcatcga tttatttcaa agccacccga agcgccagtc
     4201 agaccctgaa tattccttca gaaataaaag aagggcaaac cactgactgg atcaacatta
     4261 acagcgataa cgacaacaaa cgctgcgtct caaaaatcac cttttccggt catacagtga
     4321 actcatcgga tatggccacg ctgaaaatta tcggcgacga ctaacgccag aatatagaaa
     4381 ccacaaaaaa tgaatgttaa ttacctgaat gattcagatc tggattttct ccagcattgt
     4441 agtgaggaac agttggcaaa tttcgcccgg ctgctcaccc ataatgaaaa aggcaaaact
     4501 cgcctctcca gcgtactgat gcgcaacgaa ctgtttaaat cgatggaagg acatcccgag
     4561 cgacaccgcc gcaactggca gctgattgcc ggagaattac agcattttgg tggcgatagt
     4621 atcgccaaca aactgcgcgg acacggtaaa ttgtatcgcg ccattttgct cgatgtttca
     4681 aagcgattga agctgaaagc cgacaaagag atgtctacgt ttgaaattga gcaacagtta
     4741 ctggaacaat ttctgcgtaa tacctggaag aaaatggacg aggaacataa gcaggagttt
     4801 ctgcacgcgg tcgatgccag ggtgaatgag ctggaagagc tgctgccgct gctgatgaaa
     4861 gacaaattat tggcaaaagg tgtgtcgcat ttgctttcca gccaattgac ccgcatttta
     4921 cgcacccacg cagcaatgag cgtgcttggg catggtttgc tgcgcggcgc gggtctggga
     4981 ggccctgtgg gcgcagcatt aaatggggtt aaagcggtca gcggcagtgc ctatcgcgtg
     5041 acaattccag ccgtactgca aatcgcctgc ctgcgccgga tggttagcgc cactcaggtc
     5101 tgataccgga aaccggggaa aattttcatt ttcccctcaa aagatcgtag acactgcccc
     5161 actggctgat tattatgccg cgccctgaaa acactacagt tattcaggga aattatttca
     5221 ccattcattc gatgatgatt tttgaggaat tatgggcaac actaagttgg ctaatccggc
     5281 accgctgggc ctgatgggct tcggcatgac caccattctg cttaacctgc acaacgtggg
     5341 ttattttgct ctggacggta ttattcttgc catgggcatt ttctacggcg gcatcgcgca
     5401 aatttttgcc ggtctgctgg agtacaaaaa aggcaacact ttcggtttaa ccgcattcac
     5461 ctcttacggt tctttctggc tgacgctggt tgcaattctg ctgatgccga aactggggct
     5521 gaccgatgcg ccaaatgcac agttccttgg tgtctacctg ggtctgtggg gcatatttac
     5581 gctgtttatg ttcttcggca cgctgaaagg cgcacgcgtt ctgcaattcg ttttctttag
     5641 cctgaccgtg ctgtttgccc tgctggcgat cggtaacatt gccggtaacg ccgcgatcat
     5701 ccactttgcc ggctggattg gtcttatctg cggtgccagc gcaatctatc tggcgatggg
     5761 tgaagtgctg aacgagcagt ttggtcgcac cgttctgccg attggtgaat cccactaatt
     5821 gttttgagat ccccccgctc ggggggattt ttttattcgc taacttcgcg tcttgcactc
     5881 ttcggtctga atgctgcaac cacttccggt gccgtttcaa cgtatggccc ttccagcaac
     5941 tgaatgcagt acggtacgct ggcaaaaata ccgtgcacca caacgttacc ctcagcgtcc
     6001 ttcacacctt ccagcgtctc tttaatagac ttcggttgac cgggtaagtt aaggatcagc
     6061 gcctgtttgc gaatcacccc cacctgacgc gaaaggatcg cagttggtac aaaatgcagg
     6121 ctgatctggc gcatctgttc accaaagcct ggcatctcgc ggtccgctac tgccagcgtc
     6181 gcatcgggcg ttacgtcacg acgcgccggg ccagttccgc ccgtggtaag caccagatgg
     6241 caactcattt catccaccag ctcacacaac gtttgctcga tgatcgcctg ctcatcggga
     6301 attaagcggg tttccagttc aaacggcgtg gttagcgccg atgtcagcca ttcttccagc
     6361 gcagggatgc ctttatcctg ataaacgccg ctggatgcgc gatcagagat ggaaactaag
     6421 ccaatacgta aagtattcat caagattttt ccgtttaaac agtctcgtta aacagaatga
     6481 tacactgcga agggagtgac agacaggttt cagaagaggt agcgtgaccg acttcccggt
     6541 cacgctaaga atgattacag cagatcgccg atcatttttt ccagtttttc ctggtcaaca
     6601 gcaaacttac ggataccttc cgccagttta tctactgcca ttggatcctg gttgtgctgc
     6661 cacaggaact cggactcagt gatacgcgcc ggacgcgctt tcacttcacc agtgtaagac
     6721 agtttacgtt cgatagcccc ttcgctctcc gccagctctt tcagcagtgc cggtgcgatg
     6781 gtcagacggt cgcagcctgc cagttccaga atttcgccga tgttacggaa gcttgcgccc
     6841 ataaccacgg tttcataacc gtgctctttg tagtactggt agatttcaga tacagaaacc
     6901 acgcctggat cttctgccgg agcgtactct ttcttatcgg tattcgcttt gtaccagtca
     6961 agaatacggc caacaaacgg cgagatcagg aacacgcccg cttccgcaca agcacgagcc
     7021 tgagcgaagg agaacagcag ggtcaggtta cagttgatgc cttctttttc cagctgttcc
     7081 gcagcacgga taccctgcca ggtagaagcc agtttgatca gaatacgatc gttgctaata
     7141 cctgcatcgt tgtagagttt gatcaggcgt tttgctttcg caattgacgc ttcggtgtca
     7201 taggaaagac gcgcatcaac ttcagttgag atacggcccg gaaccagttt caggatttcc
     7261 agaccaatat ttacggccag tttgtcggtc gcgtccacga tctgctgcgc gcgatcgttg
     7321 ctctgctgtt tcgcccaggc gacagcatca tcaatcaact tacggtattc cggaatctgc
     7381 gctgcgttaa gaatgagaga agggttggtt gtggcatcct gcggttgata cagcttcatt
     7441 gccgcgatgt ccccagtgtc ggccactacg gtggtgtact gacgaaggga ggtcaatttg
     7501 tccgtcatga tagtatttct ctttaaacag cttgttaggg ggatgtaacc ggtctgccct
     7561 gatgatatca cgacgcaacg tcagcgcaac cgccgacaag acggttatcg ccaggcgaga
     7621 ctgtttcgga tttctgacac tgcgttgata tcgcccgcca tttttataca aaacctcatg
     7681 tatgctacgc agaagttatc aagtacctcg tagcgtatat acttcttaaa caattggtaa
     7741 cgtttacaca ggaaagtcat cgcgaccggc aataagaggg atatgcatgc cagatttttt
     7801 ctcctttatt aacagcgtcc tttggggttc ggtaatgatt tacctgctct tcggcgcagg
     7861 ttgttggttc acttttcgca ccggatttgt gcagtttcgc tacatccgcc agtttggcaa
     7921 aagtcttaaa aatagcattc atccacagcc aggcggttta acctcatttc agtcattgtg
     7981 taccagtctt gcagcgcgcg tgggtagcgg caacctggcc ggcgttgcgc tggcaattac
     8041 cgccggtgga cctggagccg tcttctggat gtgggttgcc gcatttatcg gcatggcgac
     8101 ctcgtttgcc gaatgttccc tcgcacagct ttataaagaa cgtgatgcca atgggcagtt
     8161 tcgtggcgga ccggcatggt atatggcgcg cgggctgggg atgcgctgga tgggcgttct
     8221 gttcgccgtc tttttgctca tcgcctacgg cataattttc agcggagttc aggcgaacgc
     8281 cgttgcgcga gccctgagtt tttcttttga ttttcccccg ctggtgacag gtattattct
     8341 cgctgtcttt gctatgctgg cgatcactcg cggtcttcat ggcgtcgccc ggctcatgca
     8401 ggggtttgtc ccgttgatgg cgataatctg ggtactgacc agcctggtga tttgcgtaat
     8461 gaatatcggg caacttcccc acgtcatttg gtctattttt gagagtgctt ttggctggca
     8521 ggaagcggca ggcggcgcgg cgggatatac cttaagccag gcgattacta acggttttca
     8581 gcgcagtatg ttttccaatg aggcgggaat ggggtcgacg ccaaacgcgg cagcggcagc
     8641 tgcgtcctgg cctccgcatc cggcagcgca aggaattgtc cagatgattg gcatttttat
     8701 cgacaccctg gtcatctgta cggcaagcgc catgctgata ttactggcgg gtaacggcac
     8761 aacctacatg ccgctggaag gtattcagct tatccagaag gcgatgcggg tgctaatggg
     8821 ttcctggggt gcggagtttg ttaccctcgt ggttattctg tttgccttca gctccatcgt
     8881 tgccaactac atttatgccg aaaacaatct cttcttttta cgcctgaaca cccctaaagc
     8941 gatctggtgt ctgcggatct gcaccttcgc aacggtcatc ggcggcacct tgctaagtct
     9001 tccgctgatg tggcaactgg cagatatcat tatggcctgc atggctatta ccaatttgac
     9061 cgccatatta ctgctctcgc ctgtggttca taccattgcc agtgattatc tacgccagcg
     9121 taaactcggc gtgcgcccgg tgtttgatcc gttgcgttat ccggagatcg gtcgccagct
     9181 ttctccggac gcgtgggatg atgtttcgca ggagtaatca caactatcga tcaactcatt
     9241 ctcttttttt gctaaagtcg gcataaattt cctgcaagga ctggatatgc tgattcttat
     9301 ttcacctgcg aaaacgcttg attaccaaag cccgttgacc accacgcgct atacgctgcc
     9361 ggagctgtta gacaactccc agcagctgat ccatgaagcg cggaaactga cgcccccgca
     9421 gattagctcc ctgatgcgca tcagcgataa actggcgggt atcaacgccg ctcgctttca
     9481 tgactggcag ccagatttca caccggaaaa tgcccgccag gcaattctgg cgtttaaagg
     9541 tgatgtctac actggcttgc aggccgaaac cttcagcgaa gacgatttcg attttgccca
     9601 acagcatttg cgaatgcttt ccggcttgta tggcgtactg cgcccgctcg atttaatgca
     9661 gccttatcgt ctggaaatgg ggatacgtct tgagaatgcc cgagggaaag atctgtatca
     9721 attctgggga gatatcatca ccaacaagct gaacgaggcg ctcgcagcac aaggcgataa
     9781 tgtagtgata aatctggcgt cagatgagta ttttaaatcg gtgaagccga agaaattgaa
     9841 tgccgagatt atcaagccag tgttcctcga tgagaagaac ggcaaattta agataatcag
     9901 cttctacgct aagaaagcac gcggtctgat gagtcgtttc attattgaaa atcggctgac
     9961 caaaccagag caactaactg gttttaatag cgaaggttac ttctttgatg aagcttcctc
    10021 cagcaatggc gaactggtgt ttaaacgcta cgagcagcgt taagtttgta gtcaatgccg
    10081 gatggcaatc cggcattgat atttagcgat gatgtttgtc aggccccgga cgatgatcgc
    10141 cacgatgatc attgtgcttg ttatggcgcg gcgatggcgg cggtccatat gggtgccagc
    10201 gattgcctcg ccattcataa tgttgtttcc accagccgtg gtcgcgccag tgaccgccat
    10261 cccagtaata gccacgatta tcacgatcgc ctatctgtaa ttttactgac gggactagcg
    10321 taatttccgc agcctgcgct gccatgggag cgaacagaac cagggaaagt gcgagtacga
    10381 tagattgcat ctttttcaca ggttactcct tttatcattg cccgttatgg gtcgatatcc
    10441 ggagattacg cgatgagaac ggcagttgtt attctccgca atcctaatct ctaagttacc
    10501 gcatttattg agaatttctc ctttttccct gtcgtttttc tccatatttt cttcataaaa
    10561 aagccgggct gcataaaagc aaacccggcc tgattgagat aatgaataga tgttactgat
    10621 gattcatcat caatttacgc aacgcagcaa aatcggcggg caggttatgc gaaagcaagg
    10681 gtaaatcagc acgttctgcc agctcttttg gcagatccaa cgtttcaccg agaatcgctt
    10741 ccacgctctc tttaaatttt gccggatgcg cggtgccgag gaacaagcca tattcgcctg
    10801 gattcaactg gtcacgcagc gcacgataag ctacggcagc gtgcggctcc gaggtatagc
    10861 ccagttcttt taactcacgc attgtctgtt gcgtggtttc atcatccacg gctgcatacc
    10921 ccagctcttt cagttgccag attttgcggc ggaacaactc ttccacacgc ggccagttgt
    10981 ttggctggct aacatccatc gcattggata acgtcgcctg agtcgctttg ggtgaccact
    11041 gaccgccgtg caggaaacgt ggcacggtat cattcacgtt ggtcgcagca ataaaacgct
    11101 tcaccggcag gccaagagat ttcgccagca ggcccgctgt caaatcgccg aagttaccgc
    11161 tcggcaccga gacaaccagc tggttgcgcg cttcctgcgg caactgcgcc acggcttcaa
    11221 agtagtagca aatctgcgcc agcaaacggc tgatgttaat ggagttagca gagtttagcc
    11281 ccagcgccac tttcagctct tcatcatcaa acgcctgctt caccagcgcc tgacaggcat
    11341 cgaaatcgcc gtcgatggca acagtttcga tattgccgcc caatgtacag aacagttttt
    11401 cttgcagtgg actgattttg cctcgtggat agaggataac cactttcaca ttcggtaaac
    11461 cgtagaaagc atgagccact gccgctccag tatcaccgga tgtcgcggtc agaatggtca
    11521 ctggcttatc gcccgcaata tgggtcagca tttgtgccat aaagcgaccg ccgaaatctt
    11581 taaatgccag cgttggcccg tggaacaatt ccagacaacc gacatcgctt tcaacattgg
    11641 cgaccggagc cgggaaggca aacgccgcac gtacgcgctc ttccaggatt tcctgcggga
    11701 tttcatcacc aataaacgcc gagaggatct tcgcactgcg ggtgacaaaa tccagcttca
    11761 gcatctcatc aatttcagtc aggctgaatt ccggcaggtc gtgcggaaaa aacagcccct
    11821 gatttttgcc taacccctgg gttacggctt gcgcaaagct gacctgctca ttgtgatctt
    11881 tcagattgta gagtttcatt tagttttcca gtactcgtgc gcccgccgta tccagctggc
    11941 aaatatgaac aaaaccttcc tgattttgca ggtagttctt acccaaccag tcggcaacgc
    12001 gctgagcggt atccggcttg tcacacagag cgaacaaggt cgggccggag ccggagatac
    12061 cgctcgcaac cgcgccgatt tccgcaaccg cctgccgcgc ctgccggaag ccaggcaata
    12121 accgttcacg gtagggttca gcgataacat ctttcatcag cttcgcggca agctcaggct
    12181 gacgggaata gcaggcgtga atgaatcctg ccaaatgtcg cccgtgcgca atgcaatcct
    12241 ggcggcgata ctgcgccggt aaaatagccc gggcttctgc cgtcgagact ttaatccccg
    12301 gatacgccag cacccacagc cactcatcaa accctggcac ttgctgactg atgatgtcgt
    12361 tttcttcaat catcaactgc ataccaccaa gaaaacacgg tgccacgttg tcgtaatgaa
    12421 tgctgccgga gatacgccct tccaactcgc ccatcaaagc cagcaaacga gtgtcattaa
    12481 gcggcttgcc gcagtgttca ttcatcgcca tcagcgccgc gaccactgaa caggcgctgg
    12541 agcctaagcc cgaaccgatc ggcatattct tttccagagt catcgccact ggaatttgct
    12601 tgccaagctc ctggcaaaaa cgctcccagc actgataaac gatattttcc cgtggctctg
    12661 acggcagctt atcggcaaag cgtccgaggt tgttgagact gaatgtctct gctgcctcaa
    12721 ccgtgactac atctccgagc aatgcaccat caacaggtgt caccgccgcc ccgagcacat
    12781 caaacccgac gctcatattg gcactggaag ccggggcata aactttaacc atgtcagact
    12841 cctaacttcc atgagagggt acgtagcaga tcagcaaaga caccggcagc tgtaacgtca
    12901 ttgcccgcac catatccgcg cagtaccaac ggcagcggct gataatagtg gctatagaag
    12961 gccagggcgt tttcgccatt tttcactttg aacagcggat cattaccatc cacttcggca
    13021 atcttcacgc ggcagacgcc atcttcatca atattgccaa catagcgcaa aacttttcct
    13081 tcatcacgag ccttcgccac acgcgcggca aagagatcgt cgagctgtga cagattcgcc
    13141 ataaaagcgg caacatcacc ctcggcgtta aactctgcgg gcagcacagg ttcaatttca
    13201 atatccgcca actccagttc acgtcccgtt tcacgagcga gaatcaatag tttacgcgcc
    13261 acatccatac cagaaagatc atctcgcggg tccggttcgg tataacccat ttcccgcgcc
    13321 agtgtggtcg cctcggagaa actcatgcct tcgtctaact tgccgaagat ataagaaagc
    13381 gaacctgaaa gaatgccgga gaacttcatc aattcatcac cagcattgag cagattttgc
    13441 aggttctcaa taaccggtaa tccagcccca acgttggtgt catagaggaa tttacgccgc
    13501 gatttttccg ccgcataacg caactgatgg tagtaatcca tcgacgaggt gttggccttt
    13561 ttgttcggcg taacaacgtg gaaaccttcg cgcaggaagt cggcatattg atctgccaca
    13621 gcctggctgg aagtacagtc aacaatgacc gggttcagca gatgatattc tttcacgagg
    13681 cgaattaagc gcccgagatt aaacggctct ttggcttgcg ccagttcttc ctgccagttt
    13741 tccagattaa ggccatgtac attggtgagc agtgccttcg agttagcaac accgcagaca
    13801 cgtaagtcga tatgtttatt cttcaaccag ctttgctgac gcttcagttg ctccagcagc
    13861 gcaccgccaa cgccaccgac gccaatcaca aacacttcga taacctgatc ggtattgaac
    13921 agcatctgat gagtaacgcg cacgccagtg gtcgcatcat cgttattgac cacgacagag
    13981 attgagcgtt cagaagatcc ctgagcaatg gcgacaatat tgatgttggc gcgggccagc
    14041 gcggcaaaga atttcgccga gatcccacgt aaggtgcgca taccatcacc taccaccgag
    14101 ataatggcca gccgttccgt caccgccaac ggctccagta agccttcttt cagttccagg
    14161 tagaactctt cctgcattgc ccgttcagct cgcacacagt cgctttgcgg aacgcagaaa
    14221 ctgatactgt attcggaaga tgattgcgta atcagcacca cggaaatacg ggcgcgtgac
    14281 atcgctgcaa agacgcgcgc cgccatgcca accatccctt tcatccccgg gccggaaacg
    14341 ctgaacattg ccatgttatt cagattggaa atgcccttga ccggtaattc gtcttcatca
    14401 cggctggcac caatgagcgt acctggtgct tgaggatttc cggtattttt aatcaggcaa
    14461 gggatctgga actgggcgat gggggtaatg gtgcgggggt gaagaacttt agcgccgaag
    14521 taagaaagct ccatcgcttc ctgataggac atcgacttca acaacctcgc atcgggcacc
    14581 tgacgcggat cgcaggtata aacaccgtca acatccgtcc agatctcgca acaatcggcg
    14641 cgtaaacagg ccgccagcac cgcagcggag tagtcggaac cgttgcgtcc cagaaccacc
    14701 agctcgcctt tttcattacc ggcagtgaaa ccagccatca gcaccatgtg gtcagccgga
    14761 atgcggcttg ccgcaatacg gcgggtggat tcagcaatat caacggtaga ttcgaggtaa
    14821 tgacccactg ccagcagttt ttcgaccgga tcgataacgg taacgttgtg accacgcgct
    14881 tctaacacgc cggccataat ggcgatcgac attttctcgc cacggcaaat cagcgcagcg
    14941 ttgatgctat ccgggcactg ccccaacaaa ctgatgccat gcaggacatg ttttatttgg
    15001 gcaaattcct ggtcgacgaa agttttcaat tgtgccagcg gaaatcccgg ctgggcggcg
    15061 gcgagtcccg tcagaagttc ggcaaaaata cgttcggcat cgctgatatt gggtaaagca
    15121 tcctggccgc taatagtttt ttcaatcatc gctaccagat ggttggtaat tttggcgggg
    15181 gcagagagga cggtcgccac ctgcccctgc ctggcattgc tttccagaat atcggcaacc
    15241 cgcagaaaac gttctgcatt tgccactgat gtaccgccga acttcaacac tcgcatggtt
    15301 gttacctcgt tacctttggt cgaaaaaaaa agcccgcact gtcaggtgcg ggcttttttc
    15361 tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat ggtggtggta
    15421 atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat ctgtctgtgc
    15481 gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt taatttgatc
    15541 acggcaggta accagttcag aagctgctat cagacactct ttttttaatc cacacagaga
    15601 catattgccc gttgcagtca gaatgaaaag ctgaaaatca cttactaagg cgttttttat
    15661 ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt gtctcgttgc
    15721 tctaaaagcc ccaggcgttg ttgtaaccag tcgaccagtt ttatgtcatc tgccactgcc
    15781 agagtcgtca gcaatgtcat ggctcgttcg cgtaaagctt gcagttgatg ttggtctgcc
    15841 gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta attgatagca atagaccatc
    15901 accgcctgcc ccagattgag cgaaggataa tccgccacca tcggcacacc agtaagaacg
    15961 tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa caccagagcg
    16021 gcatggctca tccatgaaga tttttcctct aacagcggca ccagttctac tggcgtagcg
    16081 tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc gacatcgtgt
    16141 aacgattcag ctagtgtctt gaaaacttta atattatcaa taatatcacc agatccatgt
    16201 gcgacccaac gggcggctgg ctccaggtac gcctgactat cgacaatccg cagttcgcta
    16261 aaacccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct ggcgggtgca
    16321 accagaataa tcgttatacg catattgcca ctcttcttga ccaaataacc gcgaacaggg
    16381 tgatcaccgt caacttatta cgcggtgaga atttacaaat tcttaacgta a
//
LOCUS       DACADF010000106        16418 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.108, whole genome shotgun sequence.
ACCESSION   DACADF010000106 DACADF010000000
VERSION     DACADF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.108"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            380..1168
                     /gene="fabI"
                     /locus_tag="ISB30_003294"
     CDS             380..1168
                     /gene="fabI"
                     /locus_tag="ISB30_003294"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP1990896.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            1313..2440
                     /locus_tag="ISB30_003295"
     CDS             1313..2440
                     /locus_tag="ISB30_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAP1990897.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDPLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVCDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLANTLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMNSSNEYKKISPLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            2508..4442
                     /gene="rnb"
                     /locus_tag="ISB30_003296"
     CDS             2508..4442
                     /gene="rnb"
                     /locus_tag="ISB30_003296"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAP1990898.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(4519..4622)
                     /locus_tag="ISB30_003297"
                     /pseudo
     CDS             complement(4519..4622)
                     /locus_tag="ISB30_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4676..6661
                     /gene="pdeR"
                     /locus_tag="ISB30_003298"
     CDS             4676..6661
                     /gene="pdeR"
                     /locus_tag="ISB30_003298"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAP1990899.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            6809..6982
                     /locus_tag="ISB30_003299"
     CDS             6809..6982
                     /locus_tag="ISB30_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990900.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            7071..7820
                     /locus_tag="ISB30_003300"
     CDS             7071..7820
                     /locus_tag="ISB30_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1990901.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEQSKLTVHVVDI"
     gene            8089..8307
                     /gene="osmB"
                     /locus_tag="ISB30_003301"
     CDS             8089..8307
                     /gene="osmB"
                     /locus_tag="ISB30_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAP1990902.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8433..8759)
                     /gene="yciH"
                     /locus_tag="ISB30_003302"
     CDS             complement(8433..8759)
                     /gene="yciH"
                     /locus_tag="ISB30_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAP1990903.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSMLEAKGMK
                     VKLAGG"
     gene            complement(8759..9496)
                     /gene="pyrF"
                     /locus_tag="ISB30_003303"
     CDS             complement(8759..9496)
                     /gene="pyrF"
                     /locus_tag="ISB30_003303"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP1990904.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRDFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMAAAREALVPFGKDAPLLIAVTVLTSMEACDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(9688..10857)
                     /gene="lapB"
                     /locus_tag="ISB30_003304"
     CDS             complement(9688..10857)
                     /gene="lapB"
                     /locus_tag="ISB30_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAP1990905.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKAEYAKAVESLQRVISQDRELVSETLEMLQTCYQLLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(10864..11172)
                     /gene="lapA"
                     /locus_tag="ISB30_003305"
     CDS             complement(10864..11172)
                     /gene="lapA"
                     /locus_tag="ISB30_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAP1990906.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            complement(11321..12085)
                     /gene="pgpB"
                     /locus_tag="ISB30_003306"
     CDS             complement(11321..12085)
                     /gene="pgpB"
                     /locus_tag="ISB30_003306"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAP1990907.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            12255..12845
                     /gene="ribA"
                     /locus_tag="ISB30_003307"
     CDS             12255..12845
                     /gene="ribA"
                     /locus_tag="ISB30_003307"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAP1990908.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(12909..15584)
                     /gene="acnA"
                     /locus_tag="ISB30_003308"
     CDS             complement(12909..15584)
                     /gene="acnA"
                     /locus_tag="ISB30_003308"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAP1990909.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSFKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSVLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASSELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVFLKDIWPSAQEIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCHCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(15734..15829)
                     /locus_tag="ISB30_003309"
     CDS             complement(15734..15829)
                     /locus_tag="ISB30_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990910.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(15943..16110)
                     /locus_tag="ISB30_003310"
     CDS             complement(15943..16110)
                     /locus_tag="ISB30_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990911.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(16113..16277)
                     /locus_tag="ISB30_003311"
     CDS             complement(16113..16277)
                     /locus_tag="ISB30_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAP1990912.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRNPELKRKAWLAVFLGSALFWVV
                     VALLIWKLWG"
BASE COUNT         4152 a         4227 c         3903 g         4136 t
ORIGIN      
        1 tgatgcatat tctgataatg aagagattgt ggagattctg tgaagatagt tgacaatatt
       61 aagttggagt caattcatca gaaagacagc gacttgttgt cgccagttct gttttcatca
      121 ttaaacttat aggagctaat acgaatagtt tcaaagtcag attctataag atatttcccc
      181 aacctccccc ttgcaggcgt caggagtaga taacgcaaaa tgctctgtac cgcagcttct
      241 ctccgctaca gaaagcgcaa ctatagccac ccacagccag gttgattata ataaccgttt
      301 atcagttcgt actgtttact aaaacgacga atcgcctgat tttcaggcac aacaagcatc
      361 aacaataagg attaaagcta tgggttttct ttccggtaag cgcattctgg taaccggtgt
      421 tgccagcaaa ctgtccatcg cctacggtat cgctcaggcg atgcaccgcg aaggagctga
      481 actggcattc acctaccaga acgacaaact gaaaggccgc gtagaagaat ttgccgctca
      541 attgggttct gacatcgttc tgcagtgcga tgttgccgaa gataccagca tcgacaccat
      601 gttcgctgaa ctggggaaag tttggccgaa atttgacggt ttcgtacact ctattggttt
      661 tgcacctggc gatcagctgg atggtgacta tgttaacgcc gttactcgtg aaggcttcaa
      721 aattgcccac gacatcagct cctacagctt cgttgcaatg gcaaaagctt gccgctccat
      781 gctgaatccg ggttctgccc tgctgaccct ttcctacctt ggcgctgagc gcgctatccc
      841 gaattacaac gttatgggtc tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc
      901 gaacgcgatg ggtccggaag gtgtgcgtgt taacgccatc tctgctggtc cgatccgtac
      961 tctggcggct tccggtatca aagacttccg caaaatgctg gctcattgcg aagccgttac
     1021 cccgattcgc cgtaccgtta ctattgaaga tgtgggtaac tccgcggcat tcctgtgctc
     1081 cgatctctct gccggtatct ccggtgaagt ggtccacgtt gacggcggtt tcagcatcgc
     1141 tgcaatgaac gaactcgaac tgaaataatc gttctgttgg taaatattgg gcggcgttct
     1201 gccgcccgtt atctctgtta tacctttctg atatttgtta tcgccgatcc gtctttctcc
     1261 ccttcccgcc ttgcgtcagg ataacgattt cctttacgac caaggagcgc ccatggaaca
     1321 acgccacatc accggcaaaa gccactggta tcatgaaacg caatccagta ctgcggagta
     1381 tgacgttctg cctctggtcc cggaagccgc aaaggtcagc gatccctttc tgctcgacgt
     1441 gatccttgat gaagaaacgc tggccccctt cctttcatgg ctggtccctg cgcgcgttct
     1501 tgcagtggag ttgttccctg acccgcttac cgtgactcgt tcacagacct tcaccgctta
     1561 tgaacgcttg tcgacggccc tgacggttgc tcaggtttgc ggcgtccaac ggttatgtaa
     1621 ctactattct gcgcgactta cgccgctccc cgggcctgat tcctccaggg aaagtaatca
     1681 tcggttggca caaatcacgc aatatgcccg ccaactggct agctcgcctt ctattatcga
     1741 caaccgatcg cgccagcatc tgaatgacgt tggtcttact gtctgtgact gtgtgatcat
     1801 taaccaaatc attggtttta ttggctttca ggcgcggacc attgcgacat ttcaggctta
     1861 tctcgggcat ccagtacgct ggttacccgg gctggagata caaaactacg ccgacgcgtc
     1921 actgtttgct gatgaatcat tacgctggcg aagcagctat gaggtggaaa aactccctga
     1981 agagcacaca aaaagttcaa ctgcagaact ttgccaactg gccaacacac tctctctcca
     2041 ccctatttca ctttcccttc tcgaaaagtt gttaaacagc acacgggtca atacacagcc
     2101 ggataatcag cttgcggcgt tgttatgcgc gcggataaat ggcagtcctg cttgttttgc
     2161 cgcctgtatg aattcatcaa atgaatataa aaaaatcagc ccccttctgc gcaagggcga
     2221 aaatgaaatt aaccgatggg ctgaccgtca ttctgttgag cgcgctaccg ttcaggcgat
     2281 acaatggctg acccgagcac ccgatcgctt tagcgccgcc cagttcagcc cattactcga
     2341 acacgaaaaa tcatcaacgc agattattaa tctgctagta tggagcgggc tgtgtggctg
     2401 gataaatcgc ttaaaaatcg cgttgggtga gacatattaa ccttgccgcg tcagacagat
     2461 tcgcgtaaaa ctgtcagccg ctctaatggc caccaaaata gacaattatg tttcaggaca
     2521 acccgctgct agcgcagctt aaacagcaac tgcattccca gacgccacgc gctgaagggg
     2581 tggtaaaagc cacagaaaaa ggctttggct tcctggaagt cgacgcgcaa aaaagttatt
     2641 tcattccgcc gccgcagatg aaaaaagtca tgcatggcga ccgaattatc gcggtgatcc
     2701 acagtgaaaa agaacgtgaa tccgcagagc cagaagaact ggttgaaccg ttcctgactc
     2761 gtttcgtggg taaagttcag ggcaaaaatg accgtctggc catcgttcct gaccatccac
     2821 tcttaaaaga cgccattcct tgccgcgcag cccgtggcct gaaccacgag tttaaagaag
     2881 gtgactgggc ggttgccgaa atgcgccgtc atccgctgaa aggcgatcgt tctttctatg
     2941 ctgaactgac acaatacatc acttttggtg acgatcactt tgtaccgtgg tgggttaccc
     3001 ttgcgcgtca taatctggaa aaagaagcac cagacggcgt tgctaccgaa atgctcgatg
     3061 aaggtctggt tcgtgaagat ctgactgcgc tggattttgt caccatcgac agtgccagca
     3121 cagaagatat ggatgacgcc cttttcgcca aggcgttacc ggatggcaaa cttcagctga
     3181 ttgtggcgat tgccgatcca accgcgtgga ttgctgaagg cagcaagctg gacaaagccg
     3241 cgaaaattcg cgcattcacc aactatctgc ctggcttcaa catccctatg ctgcctcgcg
     3301 agctttctga cgatctctgc tcactgcgcg ccaatgaagt ccgcccggta ctggcatgcc
     3361 gcatgacgct ctccactgat ggcaccattg aagataatat cgaattcttt gccgccacca
     3421 tcgaatccaa agcgaagctg gtgtatgacc aggtttctga ctggctggaa aataccggtg
     3481 actggcagcc tgaaagcgaa gcaattgccg aacaagtccg tttgctagca caaatttgcc
     3541 aacgccgcgg cgagtggcgt cataaccacg cactggtgtt taaagatcgc ccggattacc
     3601 gctttattct cggtgaaaaa ggtgaagtgc tggatatcgt cgccgagcct cgtcgcattg
     3661 ccaaccgtat cgtcgaagaa gcgatgattg ccgctaacat ttgtgcggcc cgcgtactgc
     3721 gcgataagct cggttttggc atctataacg tgcatatggg ctttgatccg gcgaatgccg
     3781 acgcgctggc agcgttgctg aaaacgcacg gtctgcatgt cgatgctgaa gaagtgctca
     3841 cgctggacgg tttctgcaaa ctgcgtcgtg aactggatgc gcaaccaact ggtttcctcg
     3901 acagccgcat tcgtcgcttc cagtcatttg ctgaaattag cactgaaccc ggtcctcact
     3961 ttggcctcgg tctggaagca tacgccactt ggacttcgcc gatccgtaaa tatggcgaca
     4021 tgatcaacca ccgtctgctg aaagcggtta tcaaaggcga aactgcgacg cgtccacagg
     4081 atgaaatcac tgtccaaatg gccgagcgtc gccgtctcaa ccggatggca gaacgtgatg
     4141 ttggcgactg gttatacgca cgcttcctga aagacaaagc cgggaccgac acccgtttcg
     4201 cggcggaaat tgtcgatatc agccgtggcg gcatgcgtgt tcgtttggtt gataacggcg
     4261 ctatcgcctt tattccagca cctttcttac acgctgtgcg cgatgaactg gtttgcagcc
     4321 aggaaaacgg cactgtacaa attaaaggtg aaacggctta caaagtgact gacgttattg
     4381 acgtcaccat tgccgaagtc cgcatggaaa cccgcagcat tattgcgcgc ccagtcgcgt
     4441 aatctccttt cacggcccat tcctcatgga tgggccgttt atttccccgc tcaaccttcg
     4501 tcatatcccg gcagattttt aactgacttt cgtttgaaaa ctggcgtttt tccaaaactg
     4561 gattacaaat aaatatataa aaaaacaatg tgtttagccg ccgttaattt catgtaagtc
     4621 atgttgttcc ttttatttaa ctgcggactc cgctgttaac cggaggatat gcatcatgaa
     4681 aaccgttagg gagtccacaa cgttgtacaa ctttctcgga tcgcacaatc catactggcg
     4741 gttgacggaa agcagcgatg ttttgcgctt ttctaccacc gaaatcacag aacccgatcg
     4801 tattttgcaa ctatctgccg aacaggctgc tcgcatcagg gaaatgacgg tcatcacctc
     4861 cagcctgatg atgagtctga ccgtcgatga aagcgatctt tctgtgcatc tggtaggacg
     4921 aaaaatcaat aaacgagaat ggggaggcaa cgcgtctgca tggcatgaca caccggcggt
     4981 tgctcgtgat ttatcacatg ggctttcctt tgctgagcag gtagtttctg aagcacattc
     5041 cgcaatagtg attctcgaca gccgggggaa tatccagcgc tttaatcggt tatgtgaaga
     5101 ttacacaggc ttgaaagaac acgacgtcat tgggcaaagc gtgtttaaac tgtttatgag
     5161 tcgtcgtgaa gctgcggcat ccaggcgcaa taaccgtgta ttttttcgaa gcggcaatgc
     5221 atatgaagtc gaactgtgga taccaacacg taaaggccag cggctgtttc tgtttcgcaa
     5281 taaatttgtc cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggcaccga
     5341 cattaccgaa gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat
     5401 caccggactg ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc
     5461 agataacaat aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga
     5521 cgcctatggg catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag
     5581 ctgtctcgaa catgaccagg tattggcgcg tccaggtggc gatgagtttc tggtactggc
     5641 atccaatacc tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg
     5701 gctccccttt cgcattggtt taattgaagt ttataccagc tgttcagtag gtatttcact
     5761 ctctcccgaa catggttcgg acagcgcggc tattattcgt cacgccgaca cagcaatgta
     5821 cacagcgaag gaaggcggac gaggacaatt ttgcgttttt accccagaaa tgaatcagag
     5881 ggtatttgaa tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt
     5941 ggttattcac tatcaaccga aaatcacctg gcgtggtgaa gtgcgcagtc tggaagcact
     6001 agtacgttgg cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc
     6061 cgaagaatca gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca
     6121 ggtagcgaag tggcgggata aaggcattaa cctgcgagta gcggtaaata tttctgcacg
     6181 tcagctcgcc gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt
     6241 tgaatactgc cctatagatg ttgaactgac ggagagctgc ctgattgaga atgatgaact
     6301 ggcactgtct gttattcaac aatttagccg attaggtgcg caagttcatc tggacgattt
     6361 tggtaccggc tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact
     6421 tgaccaggtt tttgttcggg atattcacaa acagcctgtc tcgcaatcac tggtccgggc
     6481 gatcgtcgct gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagaaagtgc
     6541 aaaggaagat gcttttttaa ccaagaacgg gatcaatgag cgacaaggat ttttgtttgc
     6601 caaaccgatg cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata
     6661 agtagatgaa tatatatact tagcgctata ttccgtcatt atatatcgta ttcccggatg
     6721 ataattagcc gttgctgtta aacccttctc ttttagcggc aatttttgca ttattcattt
     6781 tccccctttt gctataagga tgtccttgat gtctgaattt gatgcccagc gcgttgccga
     6841 acgtattgat attgtgttag atatccttgt ggcaggcgat taccactctg cgatccataa
     6901 tctcgagata cttaaagcag aactcttgcg ccaggttgct gaatctacgc cagacatccc
     6961 gaaggcaccg tgggagattt aacaatcgtg ccttcaggtt ttcctatttg aggatgccgg
     7021 cgcgtcagta tttttttatt tagtattata acgttataag aattacagcg atgaattccc
     7081 gacaacaaac tattctacag atggtcattg accagggtca ggttagcgta accgatctgg
     7141 caaaagccac tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac
     7201 tgagttacct ccgacgtgca catggttttg cagtttcgct tgatagtgat gacgtcgaaa
     7261 cccgtatgat gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg
     7321 ttcaaccggg cgaaaccatc tttatcgaaa atggtagcag caatgccctg ctcgcccgga
     7381 ctctgggcga gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt
     7441 tgctgaaaga cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg
     7501 aaagtatggt tggccctttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat
     7561 ttattggtat tgatggctgg caacctgaaa ctgggtttac cggtcgcgat atgatgcgta
     7621 ccgatgtggt caatgccgtg ctggaaaaag agtgcgaggc gatagtcctg accgacagct
     7681 cgaaatttgg tgctgtacat tcatactcca tcggccccgt tgagcgattc aatcgcgtga
     7741 ttaccgattc gaaaatacgc gccagcgatc tgatgcatct tgaacaaagt aaactcaccg
     7801 ttcacgtcgt tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat
     7861 catccgctct aagatgattc ctggttgata attaagacta tttacctgtt attaacactc
     7921 tcaagatata aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata
     7981 accctgcgcg cgagcggatt tcacggaata atttcacctg gcttattctt agctattata
     8041 gttatagaga gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg
     8101 agcaaaaaaa tgaccgcggc tgttctggca attaccctgg caatgtctct gagtgcctgt
     8161 tctaactggt ctaaacggga ccgcaacacc gcaatcggcg caggtgcagg ggcattaggc
     8221 ggtgcagtac tgaccgatgg cagtacgttg ggtacattag gtggtgcagc cgtcggtggt
     8281 gttattggtc accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt
     8341 ataatacgct tgattctgtt cagtacgaaa taactgaata atacacacag taaaatattc
     8401 gaaagccacg gatatatccg tggcttttta tgttagccgc ctgcaagttt taccttcatc
     8461 cctttcgctt caagcattga ttttaataaa tcacgcttat cgccctggat ttcaataatt
     8521 ccatctttta ctgctccgcc gcagccgcat tttttcttca gttcagctgc cagtttcgtc
     8581 agttcggcat catcgagatc gacaccggta atcaggcaaa cgcccttacc tttgcgtcca
     8641 ctggtctgac gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttt
     8701 ggctcatcaa tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc
     8761 atgcactccg ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt
     8821 gcgttaccgg gcgaccaatc accatataat caacaccagc cgccaacgcc tgttctggcg
     8881 tcataatgcg gcgctggtcg ccagcatcac tcccctgcgg acgaatgccc ggcgtaacca
     8941 gtttgaactc ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca
     9001 caccatcaag gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg
     9061 acagtgtcac gccaagatcg gccaggtcgc aggcttccat gctggtcaac actgtcacag
     9121 caatcaaaag cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggccatca
     9181 tacgcgcccc accggaggca tgaacattca ccatccacac gcctaagtca gccgcagcag
     9241 cgacagcgtg cgctgcagtg ttgggaatat cgtggaattt caggtcaaga aagatatcaa
     9301 aatcacgctg ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc
     9361 cgaccttcag acgacaatcg cgtgggtcga tcttgtcgac aaaggccagc gcggcatcgc
     9421 gattatgata atcaagggca acaactacag gagaattcgt aacagcgcgg gaagaagatg
     9481 aagcagttaa cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca
     9541 ggtaaacggc gagcattcta cccgtggaaa taaaaaatta acagttgcga tttctcctct
     9601 ggcaggcttt tccgcgccgt cggtaccgga acacaataga agatcaataa ttagtatgtt
     9661 gtaactaaag tcggattttt ttaaaaatta caggccatca agaccgcgaa ttggtttaat
     9721 ggttgaccag gcccgacaag acggacaatg ccagtagagg gtgtatgcgg taaaaccaca
     9781 tttctggcag cgataacgag gcttactacg taccttctcg ccaaccatgt cacgcagcac
     9841 catcagactc tctttggcac gcccttcttc tgcttcattt aagtggtaat ccattaattt
     9901 atggaacaca cgcatggtcg gatgacgctg aagctggcgc gtaatataga cctgtgcagc
     9961 ctcactaccg tcgcgcgctt cgatgatatc cgcaagcatc aattcagcat cggcaccggt
    10021 gttctcttcc accgcgcgct gcaggaattc tgcccattcg gcagttttac ccaacagctg
    10081 atagcatgtt tgcagcattt ccagcgtttc gctgaccagt tctctgtcct gggatatgac
    10141 acgttgcaaa ctttcgacgg ctttggcgta ttctgctttc gccataaaca cgcgtcccat
    10201 catgatggat acgcgggcgc tgtttttatc tgccgccgca ccttttttta gcaaagtcat
    10261 ggcacgatcg agatcgtcgc tggccatatg ctgcagagct aactcacagt agaaatgggc
    10321 aatttcgacg cgctgtttat ctttacccag cttcaccagg cgttcggcaa catcaattgc
    10381 tttctgccac tcgctggtag cctggtaaat ttgtagcagt tgttgcagcg cgccgatgcg
    10441 gaagtcagtt tcatctgtca gctgattgaa catgtcttcc gcgcggtcgt ataacccggc
    10501 agccatgtaa tcacgcccca gttgttgaat cgccaaaagc cgctgttcat aggtcagcga
    10561 ggcgctttcc attagggtct gatggatgcg aatagcgcga tcaacttcgc cacgcgaacg
    10621 gaacaggttt ccgagcgtaa ggtgggcttc aacggtacct gtatcctctt taagcatatc
    10681 gagaaacagg tctaccgctt tatcctgttg attactaagc aggaagttaa cccccgctac
    10741 gtaatcacgc gacaagcggt tggcttcatc ttgcttgttt tgttgcgcac ttctgcggcc
    10801 catataccag ccataggcag cggctacagg caaaagcaga aacaacaact ccagcataga
    10861 aagttattcc ttcgccgcag acgagtgcgg cgctacagcc acgtcagtcg cgggtgaaag
    10921 ctggttttcc agtcgcttta ttttacgttc agcgcgcgcc agggaaacac gaactcgcag
    10981 ccagaacagg ccgcaaatca accaaccgat agcaaacccc gcagcaaaca ataccgccag
    11041 caatgtggaa atacggtact ccccttgcgc taacagataa ttaaacgtca cctgttgatc
    11101 gttctgcgca cccaacgtga ccgaaatcac gaagatcgct aacaccagta agaaaatgag
    11161 taaatatttc acattacttc ccgttatgtg gttagagcga ataaattatg gtcaacttac
    11221 cgcaatagcc ctttaaagta ccattttcgc gccgtctgcg aaatggcatc cacgcgcatt
    11281 gttgatgatt taagggctaa attcggaaaa tcaaccagcg ttaactttct tgttctcgtt
    11341 gcgctatttc gcgattttct tccgcaggtg gtgttaatgg cccacaaatt cgttgcgcaa
    11401 gccacgttgc caccgccacc agcgcccacg aaatcaacgt agctacaacc agatcgcgtg
    11461 gccaatgcat cccgagcagc aggcggcttc ccatgactcc cgttgcccag accaacaaga
    11521 tagcaatggt taacgttcgc cgacgcggcc acagcaaacc aacggccagc agtgcccaac
    11581 tggcagcaaa catcgtgtga ccggaaggaa aggcaaaccc cgtctctttc tgccagtgtg
    11641 aacgcaaata ttgtgggata tttttctctt cagccaactg ttctttcacc agatttccgc
    11701 gttctgctcg ctttaaagtg tagaactcat caaccggaat atgatgtgtt ttttccagcc
    11761 agataacaaa aggtcgtggt tcctggactt tgtctttgat ccaggattta acgccttgtc
    11821 ccataaggat tgctgccgcc agaatggcaa ataacataat ggcagcctta atgcgaaaac
    11881 gcagacacca gagaaaccag ccgaataaaa tcaaatgtgt aatgacgccc cagggctggg
    11941 tgacagtttc agtaacccaa aaagccgctt ttagtaacca actttgttct ccaggttgcc
    12001 aacgccagcc agaaatccat acggctactg gcatgacaag caatagtgca gctcccactg
    12061 cggtacgtct ggcaatcgaa cgcatggcct ctcctttttg ataagtccca caatcataac
    12121 tgaaaatgcc agttccagga aaaattgaca gatttgtgcc attccgtgaa cgatcgacgc
    12181 gtcgtgatta ggtgaacccc ttctcgttat ggcaaaataa gccaatacag aaccagcatt
    12241 atctggagaa tttcatgcag cttaaacgtg tggcagaagc caaactgcca accccatggg
    12301 gcgatttcct gatggtggga tttgaagaac tggcaaccgg acacgatcat gtcgcgctag
    12361 tctatggcga tatttccggg cataccccgg tacttgcgcg cgtccattcc gaatgtctga
    12421 ccggtgacgc cctgttcagc ttgcgctgcg attgtggctt ccagctcgaa gcggcattga
    12481 cgcaaattgc cgaggaaggc cgtggtattt tgctgtatca ccgtcaggaa ggtcgtaaca
    12541 ttggtctgct gaataaaatc cgcgcttacg cactgcagga tcaaggttac gatactgtag
    12601 aggctaacca ccagttaggc ttcgccgctg atgagcgcga cttcactctt tgcgctgata
    12661 tgttcaaact ccttggcgtc aatgaagtcc gcttgttaac caataacccg aaaaaagtcg
    12721 aaattctgac cgaagcaggg attaatatta ttgaacgcgt accgttgatt gtaggtcgca
    12781 accccaataa cgaacattat ctcgatacca aagccgagaa aatgggtcat ttgctgaaca
    12841 aataaccctc ttgcattgtg taattaattt gcttgctgga agcaaaataa ccggcaagca
    12901 aatactcgtt acttcaacat attacgaatg acataatgca aaatgccgtc gttctggtag
    12961 taggtcaact ccgtcgcggt gtcgatacga caatggcagg gtacgacttc ctggctacca
    13021 tccgcgcgcg taagcgtcac cggaaccgtc gcgccgggtt gtagattttg cagatcacca
    13081 atatcaatct tctcttcccc ggtaagctct aacgttttac gcgtcacacc ttgtggaaat
    13141 tccagcggca ggatgcccat gcctattaaa ttcgaacggt gaattcgttc aaacgattcg
    13201 gcaatcacaa cacgaatacc aagcagacgc ggaccttttg ccgcccagtc acggctggag
    13261 cctgatccat actctttccc ggcaatcacc gccagcggcg tttgctcctg cttatagcgc
    13321 attgcggcat cataaataga gacgacatcg ctgtcaggta aatggcgcgt catcccccct
    13381 tcaacgccag gcaccatttc attacggatg cgaatattgg cgaaggtgcc gcgcatcatc
    13441 acttcatggt taccacgccg cgaaccatag gagttaaagt ctttccgctc aacaccccga
    13501 ccttgtagat atcgacccgc cgggctgtcg ggcttaatac tgcccgccgg agagatatgg
    13561 tcagtggtga ctgaatcccc cagcattgcg aggatccgcg caccgtgaat atcttccaca
    13621 ggtgctggtg ttgcctgcat ttcatcaaag aaaggcgata agcgaatata ggttgaatcc
    13681 tcctgccaac cgtaggtatc ggatcgtgtg acgttaattt ccttccactc ggctgtgcct
    13741 tcaaaaactt ctgcgtactc tttgcggaac atttctgtgg agacttggtc taccgcacgg
    13801 gcaatttctt gtgccgatgg ccagatatct ttcagaaata ccggctcgcc tttgcgatca
    13861 tggccgatag gctcagaagc cagattgata ttcatatttc ccgccagcgc ataggcaacc
    13921 accagcggcg gcgaggccag ccagttggtt ttaaccagcg gatggatacg gccttcaaag
    13981 ttacggttgc cggacagcac tgcaccgacg gttaaatcgc cttttttgat tgccgtttcg
    14041 ataggatcgg gcaacggtcc cgagttacca atacaggtgg tacaaccgta tcccacaagg
    14101 ttaaatccca gttcatcgag atacggtgtc agtttcgctt ttgccagata atcagaaacg
    14161 actttcgaac ccggtgccag cgacgctttg acccatggtt gccgcttgag gcccagagtt
    14221 acggcttttt tcgccagcaa gcctgcggcc atcagcacac ttgggttaga ggtgttggtg
    14281 cacgacgtta tcgcagcaat gaccacagcg ccatcaggta actgatactg atgtccgttc
    14341 ataacataat cgaccggcag gcgatcttta tgcgtggcat tcacttccag ttcgctactg
    14401 gcagcaaatg cttttggtac atcgggcagt gcaacgcgat cctgcgggcg ttttggccct
    14461 gccaggctcg cttcaacgtc attcatatcc agttctaaca cactggtaaa aattggttca
    14521 tcgcccggat tacgccacat gccctgcgct ttggcatatt tttcgaccaa ctcgacctga
    14581 tcttcactgc gcccgcttaa acgcatgtaa tcgagggtga cagcatcgat tgggaagaag
    14641 ccacaggtgg caccatattc tggcgacata ttggcaatgg tggcgcgatc cgccagcggt
    14701 agtgaatcca gcccatcacc ataaaattcg acgaatttcc ccaccacgcc atgtttgcgc
    14761 aacatttggg taacagtgag aaccaggtct gtggcggtaa taccttcacg taattttccg
    14821 gtaagtttaa agcccactac gtccgggata agcatggaaa ccggctggcc taacattgcg
    14881 gcttctgctt cgatgccacc aacgccccac cccagcacgc caaggccgtt gatcatggtg
    14941 gtgtgcgagt cagtaccaac gagtgtatcc ggataagcaa tccattcacc gtcctgcaat
    15001 tcgctccaca ctgctttgcc gagatattcg aggttaacct gatggcaaat gcctgtgcct
    15061 ggcggcacga cgctaaaccg actgaacgcc tgctttcccc atttcaggaa cacataacgt
    15121 tcgtggttgc gctccatttc caggcgtacg ttttcttcaa atgcctcatc atcaccaaaa
    15181 cgatcgacgg tcaccgagtg gtcaatgacc aggtcgaccg gtgagagcgg attaaccttt
    15241 gcagtatcgc cgccgaggcg tttaaccgct tcgcgcattg ccgccagatc aacaacggca
    15301 ggtacgccgg taaagtcctg catcagcact cttgccgggc ggtaggcaat ttcacggtca
    15361 gcatgggcat ttttcagcca tcccgccagc gcgtggatat cctcttcggt aaccgattta
    15421 ccatcctgcc aacgcagcag gttttcgagc aaaactttga atgacttggg tagacgggtg
    15481 atatcgccca gtgatttggc agcaagcggc aagctgtagt agtggtaagt tttatctttg
    15541 gcctgcaacg tgtccttact ggcttctcgt agggttgacg acatagctcc tccttaatga
    15601 cagggttgcg aagccctgat tctcttcagg gtgaatatta aagataacac aaacatcgcg
    15661 taacgatttg ataacaaccc aaattgataa aagaggagaa accttgaggc aaagcaaaca
    15721 gcgggatcag ggatcagtga gtaatgatcc agaggagttc ggcccagaag agcatcgaca
    15781 gagaaaatgc gcccatccag gaccaatatt tcatatttgt gttgatcata ggctacaccc
    15841 ttaacggtta ttactttgtg ttgagataac tcaacctgtt attgctgcgg tgtattaaaa
    15901 gccctgtaat tcattaaaaa tgggtaataa gaatgatgat aattattgtt tcggctgatc
    15961 atcttcagca aacagttcta taaaggcttg ttgctgcgat gttagttgcc agtcgtctgg
    16021 aataacgacg gaacattccc gttttgcttc tggttcattg tcactatgct gacataatgg
    16081 tgacgactcc agttgctcct gaccgaccat ggttatcccc acaatttcca aataagcagt
    16141 gcgacaacca cccagaaaag tgcagaacca agaaaaaccg ccagccaggc tttacgtttt
    16201 aattcaggat tgcggcgggg ttcctgattt ccagaaggca ttgctaacct catataatcg
    16261 ccatcactta tcagcaagac gttgaacgat tggtgtaatt aatcatacga tgagacaaat
    16321 cctaaagaaa ctttagtcaa aaatccagcg aatttacgaa tcgtctccag tgaataattc
    16381 aatcttttga cctgaaatag atatttgaaa actggagt
//
LOCUS       DACADF010000107        16334 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.109, whole genome shotgun sequence.
ACCESSION   DACADF010000107 DACADF010000000
VERSION     DACADF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.109"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(59..139)
                     /gene="shoB"
                     /locus_tag="ISB30_003312"
     CDS             complement(59..139)
                     /gene="shoB"
                     /locus_tag="ISB30_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAP1990913.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLLLLFL"
     gene            complement(560..940)
                     /locus_tag="ISB30_003313"
     CDS             complement(560..940)
                     /locus_tag="ISB30_003313"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP1990914.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(940..1671)
                     /gene="pdxJ"
                     /locus_tag="ISB30_003314"
     CDS             complement(940..1671)
                     /gene="pdxJ"
                     /locus_tag="ISB30_003314"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAP1990915.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVCILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1683..2411)
                     /gene="recO"
                     /locus_tag="ISB30_003315"
     CDS             complement(1683..2411)
                     /gene="recO"
                     /locus_tag="ISB30_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP1990916.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2423..3328)
                     /gene="era"
                     /locus_tag="ISB30_003316"
     CDS             complement(2423..3328)
                     /gene="era"
                     /locus_tag="ISB30_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP1990917.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3325..4005)
                     /gene="rnc"
                     /locus_tag="ISB30_003317"
     CDS             complement(3325..4005)
                     /gene="rnc"
                     /locus_tag="ISB30_003317"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP1990918.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4277..5251)
                     /gene="lepB"
                     /locus_tag="ISB30_003318"
     CDS             complement(4277..5251)
                     /gene="lepB"
                     /locus_tag="ISB30_003318"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP1990919.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5267..7066)
                     /gene="lepA"
                     /locus_tag="ISB30_003319"
     CDS             complement(5267..7066)
                     /gene="lepA"
                     /locus_tag="ISB30_003319"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP1990920.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQIDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7264..7743)
                     /gene="rseC"
                     /locus_tag="ISB30_003320"
     CDS             complement(7264..7743)
                     /gene="rseC"
                     /locus_tag="ISB30_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAP1990921.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7740..8696)
                     /gene="rseB"
                     /locus_tag="ISB30_003321"
     CDS             complement(7740..8696)
                     /gene="rseB"
                     /locus_tag="ISB30_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAP1990922.1"
                     /translation="MKQLWFAMSLVTGSLLFSANALATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGTAQ"
     gene            complement(8696..9346)
                     /gene="rseA"
                     /locus_tag="ISB30_003322"
     CDS             complement(8696..9346)
                     /gene="rseA"
                     /locus_tag="ISB30_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAP1990923.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9379..9954)
                     /gene="rpoE"
                     /locus_tag="ISB30_003323"
     CDS             complement(9379..9954)
                     /gene="rpoE"
                     /locus_tag="ISB30_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAP1990924.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9951..10106)
                     /gene="rseD"
                     /locus_tag="ISB30_003324"
     CDS             complement(9951..10106)
                     /gene="rseD"
                     /locus_tag="ISB30_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAP1990925.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10362..11984
                     /gene="nadB"
                     /locus_tag="ISB30_003325"
     CDS             10362..11984
                     /gene="nadB"
                     /locus_tag="ISB30_003325"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAP1990926.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVGKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDISRRIPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSAGNHYINR"
     gene            complement(11969..12706)
                     /gene="trmN"
                     /locus_tag="ISB30_003326"
     CDS             complement(11969..12706)
                     /gene="trmN"
                     /locus_tag="ISB30_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAP1990927.1"
                     /translation="MSQSTSVFRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADILQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12838..14172
                     /gene="srmB"
                     /locus_tag="ISB30_003327"
     CDS             12838..14172
                     /gene="srmB"
                     /locus_tag="ISB30_003327"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAP1990928.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14205..15086)
                     /locus_tag="ISB30_003328"
     CDS             complement(14205..15086)
                     /locus_tag="ISB30_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1990929.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            15189..15776
                     /gene="eamB"
                     /locus_tag="ISB30_003329"
     CDS             15189..15776
                     /gene="eamB"
                     /locus_tag="ISB30_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAP1990930.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(15832..16215)
                     /gene="grcA"
                     /locus_tag="ISB30_003330"
     CDS             complement(15832..16215)
                     /gene="grcA"
                     /locus_tag="ISB30_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAP1990931.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
BASE COUNT         3956 a         4340 c         4006 g         4032 t
ORIGIN      
        1 gttattaaag gcgcgtccga ttggttcacc ggacgcacct taagtacgtt tccttgtgtt
       61 ataagaacaa aaggagcagc tgtaaaacag caatgatgat tttgatgacc cgtttaatca
      121 ggtatcggca atcagtcatt cgtttttcct taaacaagga atgcagccgt tcagattagc
      181 ccttacatct ccccaaaact gaacgtgcga gttattggaa tcgagtgctg cactccacac
      241 cagagctttg acgacaccac tcgtttcaat gggggaattc agtggcatgg tgtaaagcac
      301 agcaaaattg ccaataacga aaccgatttt aatgtactta tcaaaaagcg gtcacgttaa
      361 tcttttcatc aatgtcgatt tcttcgtaaa ggctcgcgtt ttttatgtca gagattgcaa
      421 gttgcttgta caggtcaacg aaattgatcc ataatcattt tcgttggaat cgtgtgctgc
      481 acaaaattaa agttaaaaag taaaaccccc gttccttacc agttcggggg ttttacttta
      541 taaagagaac ggtattattt taactttcaa taattaccgt ggcacaagca tagtggcgct
      601 catcggcaag cgttacatgc atatttacaa cgccgagctt ttccgccagt tttaatgcct
      661 caccccatag ccgtagccgt ggtttgccga gctcatcatt gaatacttca aactgattaa
      721 acgccagacc attgcggatc cctgtgccaa atgcttttgc cgccgcttct ttcacagcaa
      781 aacgcttcgc cagaaaacgc actggctggt ggtgcgtttt ccagatttcc cattcgttat
      841 cgcttaatac gcggcgtgcc aggcgctcac cggatcgggc gatcaccgct tctatgcgag
      901 cgatctctac gatatccgta cctaaaccta atattgccat tagccacgcg cttccagcat
      961 cagacgcttc atttctgcca ccgcatcttt cagtccggtc atcactgcac gaccaataat
     1021 ggcatgaccg atattcagtt catgcatctc agggatggcg gcaatggctt tcacgttgtg
     1081 ataggtcaga ccgtgtccgg cgttaacttt cagaccgaga ctagtggcaa aggtcgcggc
     1141 tttggcgata cgcgccaact cttgcgcctg ttcggcgtca gttttggcat cagcatagca
     1201 accggtgtgg atctcgataa acggtgcgcc aacctctgcc gcagctttga tctgctcttc
     1261 atcggcgtcg ataaacagag aaacctggat cccggcatct gccagacgtt tgcaggcatc
     1321 gcgcattttt tcacgctgcc ctgcgacgtc caggccgcct tcggttgtta cttcctgacg
     1381 cttttccggt accagacagc aaaaatgtgg cttcgtctca acggcgatcg ccagcatctc
     1441 ttcggtcacc gccatctcca gattcatgcg ggtatccagc gtctggcgca ggatgcacac
     1501 atcgcggtcg gtaatgtggc ggcggtcttc acgtaaatgc acggtaatgc cgtccgctcc
     1561 cgcctgctca gcaataaacg ccgcctgcac cggatccggg taagcagtac cgcgcgcgtt
     1621 acgcagtgtt gcaatatggt caatgttgac gcctaacagt aattcagcca tgacaatcct
     1681 catcattcat aatgtgtttt caccgttcgc ttaggcataa actgccggaa cagttccctg
     1741 ctctttaatg gtttaccgcc aagatacggc ttaagcgcca tgcgggtaaa gcgtttcgcg
     1801 gcgcgcagtg tgtctgcgtc aggaaattcc cgcgcgttta acgcttttaa ctgccttccg
     1861 gtgaacgttt tattgtcgat aacgacgctt gcgataaacc ctttttcttc gcgataacga
     1921 tacgtcatgg tgtcatctac cggctcgccg ctacccgcac aatgggtaaa attgacgcca
     1981 tagcccagat gcccgagcag tgccagttca aagcggcgca acgcgggttc tggcgtacca
     2041 gtgaccccgg caagagactg aatgcagtgc aagtaatcga aaaagagttc agagaagcgc
     2101 gtctcgtatt ccagtacgcg ggagagaagt tcgttgatgt acagaccgct gtaaagcgtg
     2161 ataccgctta atggcagcgc cagcgagaca gcttcagcac tgcgcagcgt tttgacttcg
     2221 ccacgcccgc caaaacgtag caagagaggg gtgaaaggct gtaatgcacc tttcagagta
     2281 gagcgtttag agcgtgcgcc tttggcaacc agacgcacgc gacccgattc ctccgtgaag
     2341 acgtccagca tcaggctggt ttcgctccac gggcgactat gcaggacaaa tgcgcgctgc
     2401 cagccttcca tcggagttac tcttaaagat cgtcaacata accgagactg cgcagtgcgc
     2461 gttcgtcgtc ggcccaaccg gatttcactt ttacccacag ctcaaggtga acaggcgctt
     2521 cgaacatttc ctgcatgtct ttacgcgctt caatcccgat ggttttgatc ttggcccctt
     2581 tgttgccaat gaccatcttc ttctgccctt cacgctcaac gagaatcaaa ccgttgatgt
     2641 cataaccgcc gcgttcgtta gagacgaaac gttcgatctc cacggtcacg gagtacggca
     2701 gttcagcgcc gagaaaacgc atcagttttt cgcggataat ttcagacgcc ataaaacgct
     2761 gtgagcgatc ggtgatgtaa tcttccggga agtgatgggt cgcttcaggc agatgcttac
     2821 gcacgattgc tgcaatagtg tcgacattca gcccggtttc ggcagagatc ggcacgatat
     2881 cgaggaagtt catctggctt gccaggaact gtaagtgtgg cagcagatcg gctttttcct
     2941 gcacattatc cactttgttc accgcgagga ttaccggcgc tttgccatcg cgcagtttgt
     3001 tgagcaccat ttcatcgtcc ggcgtccagc gagtgccttc tacgacaaaa atcaccagct
     3061 caacatcgcc aatggagctg ctcgccgctt tgttcatcag acggttaatg gcgcgttttt
     3121 cttccatatg caggcctggg gtatcaacgt agatcgcctg atacgcgcct tcagtatgga
     3181 tccccacaat gcggtgacga gttgtttgcg ccttgcggga ggtaatggag atcttctgcc
     3241 ccagcagttt gttcaacaat gtggatttgc caacgttcgg acgtccgacg atagcaataa
     3301 atccgcagta acttttatcg atgctcattc cagctccagt tttttcaacg cctgttcggc
     3361 ggcagcctgc tcagccttac gacggcttga acctgtgcca accaccggtt cactcaggcc
     3421 gctgacctgg cagtggatag taaattcctg atcgtgcgct tcgccacgta cctggactac
     3481 cagataagtt ggcagcggca gatggcgacc ctgcaaatat tcctgcagac gcgttttcgg
     3541 atctttttgt ttatcgcctg ggctaatttc gtccagacga gtttgatacc agttgaggat
     3601 taatttctcg acggtttgaa tatcactgtc gaggaatacg ccaccaatta atgcttcgac
     3661 ggtgtcggcg agaattgact cacgacgaaa tccaccgctt ttaagttcac ctggccctaa
     3721 acgtaagcac tcacctaact caaattcgcg cgccagttca gccagcgtat taccacggac
     3781 cagcgtggcg cgcatccggc tcatatcgcc ttcatccaca cgagggaaac ggtgataaag
     3841 cgcattggcg ataacgtagc tcagaataga gtcacctaaa aattccagac gctcgttatg
     3901 tttactgctg gcactacgat gagttaatgc ctgctgcaac agttcctgat gattaaaagt
     3961 gtagcccagc ttccgttgaa gccgattaat tacgatgggg ttcatgcgat accaataaat
     4021 aaatgcgtca acaattcagc acacgaaaca gacctgatat acatggctcg gctaactgct
     4081 tcactgcagt ttcagtttac cggtatatgg ggaccaacgc tgtttcgtgt gccgtggcaa
     4141 cctggaggtg ccaaccttaa acttcggggg aatattctat acacaacgac gggggatgtc
     4201 gttagccacg ggagatttat ctcataaata attcacgttg tcgccataac ggcgacaacg
     4261 tgaacgaaga tggctattaa tggataccgc caatgcgact taagcgcaca cctgtcggcc
     4321 attcgccttc ttgcttatcg aagctcatcc agatagccgt tgcccgaccg accagattcg
     4381 cttcaggcac aaagccccag taacgactgt ccgcgctgtt gtcgcggttg tcgcccatca
     4441 tgaagtattg tcccggcgga acaatccagg ttgccagttg ttgccctggc tgctggtaat
     4501 acatccccac ctggtcctgc gcaatcggca ctgtcagaat acggtgcgtc acatcaccca
     4561 gtgtctcttt acgctcggaa agacgaattc cattttcttt ggtttcgttt ttcggcactt
     4621 caaagaatcc gctggtcgct tccccaccat tacggcgtga gaaggtctga acgaaatcgc
     4681 tcggttccac gttggagtaa gtgaccggta gcgcgttttc acacgcctgg ccggaactgc
     4741 atcccggttg aatcgtcagc tcttttgaga ccggatcata agtgacttta tcgcccggta
     4801 aacccaccgc gcgcttgatg taatcaagct ttggatcttc cgggtattta aagaccacga
     4861 tatcgccgcg tttcggatga ccggtttcga tcagcgtttt ctggtagata ggatctttaa
     4921 tgccataagc aaacttctct accagaataa aatcaccgat taacagagtc ggcatcatcg
     4981 aacctgacgg gatctggaac ggttcataaa taaacgaacg cacaatcaat acgatagcca
     5041 gtaccggaaa aacagaagca ccggtttcca gccagccagg cttcggcgca acctttttca
     5101 gcgttgcttt atccagtgag tcaccggcag ccgcctgcgc cgctgcctga cgttcccgcc
     5161 gtttaggtgc gaaaaagaat ttatccacgc accataaaat gcccgtcacc agtgtggcaa
     5221 tcaccagaat cagggcaaac atattcgcca tgccaactcc taagggttat ttgttgtctt
     5281 tgccgacgtg cagaatggcg aggaacgctt cctgcggcag ctcgacgtta ccgatctgct
     5341 tcatgcgttt cttaccttct ttctgcttct gcagcagctt tttcttacgg ctgatatcgc
     5401 cgccataaca tttagccagt acgtttttac gcagctgttt cacggtggag cgcgcaatga
     5461 tgtgcgtacc aatcgctgcc tgaatggcga tatcaaactg ctggcgtggg atcagatctt
     5521 tcatcttctc caccagctcg cgaccgcggt tttgcgaatt accacggtgg gtgatcagcg
     5581 ccaatgcatc aacacgttca ccgttgatta atacgtctac acgtaccatg tcggacgcct
     5641 ggaagcgttt gaagttgtaa tccagagacg cataaccacg cgaggtagat ttcaggcgat
     5701 cgaagaagtc gagcaccact tccgccatcg ggatctcgta cgtcagcgcc acctgattac
     5761 cgtggtaaac catattggtc tgcacgccgc gtttttctac gcacagcgta ataacgttgc
     5821 cgagatatgc ctgcggcagc agcatgtgac actctgcaat cggctcgcgc agttcgtaaa
     5881 tgttatttac cgctggcagc ttggatgggc tgtcgacgta gataacttct cttgacgtgg
     5941 tttcaacttc atacactaca gtcggcgcag tggtgatcag atccagatcg tattcacgtt
     6001 ccagacgttc ctggatgatc tccatgtgca gcaggccaag gaagccgcag cggaaaccaa
     6061 agcccaacgc gctggagctt tccggctcat agaacagtga ggcatcgttc aggctgagtt
     6121 tacccagcgc gtcacggaag gcttcatagt cgtcggaact taccgggaac agaccagcat
     6181 atacctgagg tttgactttc ttaaagccag gcagcgcctt ttctgccgga ttacgcgcca
     6241 gtgttaaggt atcgcctact ggagcgccgt ggatatcttt aatcgcacat accagccagc
     6301 ctacttcgcc acatttcagt tcagtgcggt caatctgctt cggcgtgaag atgcccagac
     6361 gatcggcgtt ataggtctgc ccggtgctca tgactttcac tttgtcgccc ttacgcaggg
     6421 tgccgttttt aatacggata agtgaaacaa cgcccaggta gttgtcgaac caggagtcga
     6481 taatcagcgc ctgcaatggg ccttccggat cgccttccgg cggcggaatg tcgcgcacca
     6541 agcgttcgag aacgtcttgc acgccaacgc cggttttcgc tgaacagcgc accgcgtcgg
     6601 tggcgtcgat gccgacgata tcttcaattt cttccgccac gcgttcagga tcggctgccg
     6661 gcaggtcaat cttgttcagt accggcacca cttcgagatc catttccatg gcggtgtagc
     6721 agtttgccag ggtttgcgct tctacgccct gcccggcgtc gaccaccagc aatgcacctt
     6781 cacaggcagc cagcgaacga gaaacttcat aggagaagtc tacgtggccc ggggtgtcga
     6841 taaagttaag ctgataggtt tcgccgtcag acgctttgta gtccagcgtc acgctttgcg
     6901 ctttgatggt aatgccacgc tcacgctcaa gatccatgga atcgagaacc tgcgcctcca
     6961 tttcacggtc agacaggcca ccgcagatct ggataatacg gtcagacagc gtcgatttac
     7021 cgtggtcaat gtgagctatg atcgaaaagt tacgtatatt cttcataaag tatgattatt
     7081 gtgccttaat gcccggttaa ccaggctttt agaagtcgct gttctgagct taacgtctgt
     7141 attaatagaa acgccgcatt ctacactaca acattgaggc gaggaaatgt tcataccgta
     7201 tggattgtgg tatatggaaa cgtcctcgca tttgttatgc aaaatgcaac aaagccagtg
     7261 aaatcactgg ctcgcgtctt ccgaagatgt ttcaaatcgc acaaggccag gcggcaatgc
     7321 cacgcttaag atgatcggtt gccattctgc ccgggcagca aacttacgcg aatagccgcg
     7381 cgcaatcagg aacccaccaa taccgccgag gatcgcacca cataatgctg cgacgtcaga
     7441 agcaaagagt agctgaaata gcgaagcgat gaggaataat cccaccagcg gcgacatata
     7501 aaccagtaat gcggagctaa gaaggctgcc ttcggcgatc cccaattcca ctttttgccc
     7561 cggcactagc ggctcatcac agggtacgac aatggtatgc gtggtttgcg ggccaagttt
     7621 attcaacaca cggctaccgc aaccggcgcg tgaagcgcag ctgctgcatg aggctttaac
     7681 atcacaactg accagcgcct gcccgttttc ccaggagacg acggtagccc actctttgat
     7741 cattgcgctg tcccgaactt aatattctcg gcaatgcgtt tcgccgtttg cggcggcagt
     7801 tcaccaacaa tggtgatttc ggcgttatca cgtacgcttg tactgacggt tctgcgtccg
     7861 gtgcgcaaca tctgatcggt gctcgatggc gtagcgcggt taacgtttac cgagaagctg
     7921 aataatccgt cggaatagag acgtgattcg ataggcatgt tgtccatcgt cggtagcgga
     7981 cgtcgactac tggaaacttc gctaaaaccc tgtggcagcc aggttggcgt ccagctgaat
     8041 ttagcttttt caccaacagg aacagaaagc aacggcggca aatttgcctt cgccagcgtc
     8101 tgcatactgc tgccgatatc ctgattgacg ttaaaagcaa tcacgcgaaa ttgttccagc
     8161 gtttcaccat cgcgatcaag gagatcaacc cgcatcggta atttcgattc ggtgtccatc
     8221 cacacgatgt agctgtagcg tgtaccatct cgggcaacca cacgaatgac ttcgcaaaga
     8281 cgatcagcaa tacgcgtgcg cccgacagag ataaagtcgt agtaaggaga aaggcgtttg
     8341 aaatcggtat agatgagcga tggcagagaa tcaacaatgt aatcgccatt aagcgtgaac
     8401 ggttcaagtc ccggttcaaa atagctgatt tcattgccgc gctgtaccac ttcccggcgc
     8461 gggccatcca tttgcaacaa ttgtgcaaga ggacggttat cgaggcgtgc atgtcgataa
     8521 cgcagagact caacaccctg tttattgatg ctgataaatg acagctcgta attcagtgac
     8581 tgactggcca ggttcatctg ctgtaataac gccccggacg cgggagtggc caaggcgtta
     8641 gcagagaata acaggctacc tgtcactaat gacatggcaa accaaagttg cttcattact
     8701 gcgattgcgt tcctaaagtt tgaattcctg gcacctgtac agcggcttgc tgagtttgtg
     8761 cctgctcaaa ctgaagctgt tcagagtgga gtcggcgttg cagttcgtaa tcctgcaaca
     8821 ttgcattaat gcgacgacgc tgctcctgta cctgctgctg ttgaccatta tttgcggtcg
     8881 cttcagaagg tactcccaga ctaaccgggc tggctttacc catcatcggc agtgtattaa
     8941 atactggcgt ttcgggctgc tgggacgttt cagattgccc attatagtgc tggacgccaa
     9001 cgataactgc aagcgatacg catgctgcta cgcccatttg ggtaagctgt gccgcccacg
     9061 gacgtacttt ctgccagaat ggcattttct gccattgatg cggcgcaggc tgggcttccg
     9121 ggatcaatgt cgccggttga cgtactggct cttcttcaat ggcggccatc acgcgtgaag
     9181 agatatcgaa atggagcacc tcgggagtat caccccgcat tgagtcacgg attaagtgat
     9241 agctttccca ggttttctgc atttctgggt tatgagccag ttcgttaagc agctcactat
     9301 ccagcgtttc gccatccatt aaagcggaaa gttgttcttt ctgcatgcct aataccctta
     9361 tccagtatcc cgctatcgtc aacgcctgat aagcggttga actttgttat caatagcttc
     9421 cctcgctcgg aagatacgtg aacgcaccgt acccaccgga caatccatga tagcggctat
     9481 ctcttcatag ctcaggccat ccagctcccg caaggttatt gccatgcgta aatcttccgg
     9541 gagggactca atagttcgga aaactatctg tctcagttct tctgacaaca ttaagttctc
     9601 agggttcgaa atttctttca acgcgccgcc actttcgaag ttttcagctt caatggcatc
     9661 cacatcactg gaaggtggac gacgcccctg agcaaccagg taatttttcg ctgtatttac
     9721 agcaatccga tacagccatg tataaaaagc gctatctccc cggaacgaat ccagcgcacg
     9781 ataggcttta ataaaagctt cttgtaccac atcgggaaca tcacccgacg gcacatagcg
     9841 ggaaaccaga ctcgccactt tatgctgata gcgcactacc agtaagttaa aggctttctg
     9901 atctcccttc tggacccgtt caaccaggac ctggtccgtt aactgctcgc tcatccgagg
     9961 taaagtctcc ccaaaccaaa tttccacgcg ctatcgaaac gccactccat tagctgcaat
    10021 ttgagcaagc aaagggttag agtgtctcgt ttttgtaaag ttccgtaacg catctgtttt
    10081 tgtttgtcat gctgtagacg gatcattatc tatcattata agtctacaga atctgaacat
    10141 cgcattatct gtgtagaaat gcccatttaa ctgcctgaag agtaacccaa cggccttttt
    10201 atttcaccac ctaatcctcc accagccagt aacttctctt tttctcgccg ccctgagcca
    10261 gcgtgtttag tcactgtaac aaatattaaa atagcaggtg tttatttgca caacatgatg
    10321 ttatgctgac caaacagtgt ttagtaaatt aaacaaagaa aatgaatact ctccctgaac
    10381 attcatgtga cgtgttgatt atcggtagcg gcgcagctgg actttcactg gcgctacgcc
    10441 tggctgacca gcatcaggtc atcgttctaa gtaaaggccc ggtaacggaa ggttcaacat
    10501 tttatgccca gggcggtatt gccgccgtgt ttgatgaaac tgacagcatt gactcgcatg
    10561 tggaagacac attgattgcc ggggctggta tttgcgatcg ccatgcagtt gaatttgtcg
    10621 ccagtaatgc acgatcctgt gtgcaatggc taatcgacca gggggtgttg tttgataccc
    10681 acattcagcc gaatggcgaa gaaagctacc atctgactcg tgaaggtgga catagtcacc
    10741 gtcgtattct tcatgccgcc gacgccaccg gtagagaagt agaaaccacg ctggtgggca
    10801 aggcgcagaa ccatccgaat attcgcgtgc tggagcgcag caacgcggtt gatctgattg
    10861 tttctgacaa aattggcctg ccgggcacgc gacgggttgt tggcgcgtgg gtatggaacc
    10921 gaaataaaga aacggttgaa acctgccacg caaaagcagt ggtactggca accggcggtg
    10981 cttcaaaagt ttatcagtac accaccaatc cggatatttc ttctggcgat ggcattgcta
    11041 tggcgtggcg cgcaggctgc cgggttgcca atctcgaatt taatcagttc caccctaccg
    11101 cgctgtatca cccacaggca cgcaatttcc tgttaacgga agcactgcgc ggcgaaggcg
    11161 cttatctcaa gcgcccggat ggcacgcgtt ttatgcccga ttttgatgag cgcggcgaac
    11221 tggccccgcg cgatattgtc gcccgcgcca ttgaccatga aatgaaacgc ctcggcgcag
    11281 attgtatgtt cctcgacatc agccataagc ccgccgattt tattcgccag catttcccga
    11341 tgatttatga aaaattgctc gggctgggga tagatctcac gaaagaaccc gtgccgattg
    11401 tgcctgctgc acattatacc tgcggtggtg taatggttga tgatcatggg cgtacggacg
    11461 tcgagggttt gtatgccatt ggcgaagtga gttataccgg cttacacggc gctaaccgca
    11521 tggcctcgaa ttcattgctg gagtgtctgg tctatggctg gtcggcggca gaagatatca
    11581 gcagacgtat accttatgcc cacggcgtca gtacgttacc gccgtgggat gaaagccgcg
    11641 ttgagaaccc tgacgaacgg gtagtaattc agcataactg gcacgagcta cgtctgttta
    11701 tgtgggatta cgttggcatt gttcgcacaa cgaagcgcct ggaacgcgcc ctgcggcgga
    11761 taaccatgct ccaacaagaa atagacgaat attacgccca tttccgcgtc tcaaataatt
    11821 tgctggagct gcgtaatctg gtacaggttg ccgagttgat tgttcgctgt gcgatgatgc
    11881 gcaaggagag ccgtggcctg catttcacgc tggattatcc ggaactgctc acgcattccg
    11941 gtccatcgat cctttctgcc ggcaatcatt acataaacag ataaaaagcc tgggtcagcg
    12001 ccgtatacgc ttcggaatag ttctggtctg gcccacgaat gactaagcga tcgctaaagc
    12061 actctcccgc ctgcggggag aatgccagca gcacccgatg cggcagtcgc gcttcgtttt
    12121 ccgccacatc cgtccgcaaa cgtaaatgcc agcccatact taatgccagt tcagtaaaac
    12181 cattaccaat ctgctctggc agcaccacgc agaaaaatcc ctcttcggta atgcactccg
    12241 ccgcacaagt cagcaacgat gggtgatcaa gcgtagtggt atagcgagcc tgttcccgtt
    12301 gaggtgtcgc gcactctact ccctgctgat agtaaggtgg gttgctgatg attaaatcga
    12361 agcgtactgt ctgctgtgtg atccactgca gaatatccgc cgtatggaca ttaatccgct
    12421 ctgcccacgg ggactggttg atattttcct gcgcttgcgc ggcagcttca ctttccagtt
    12481 caactgcatc aatcatcacg ctgtcatctg ttcgctgcgc cagcattaat gccagcaacc
    12541 cgctacccgc gccgatatca aggcaacgtt ttaccccagc taccggtgcc catgcgccca
    12601 gcaaaatgcc atccgttccc actttcatcg cacagcgatc gtgagcaaca aaaaactgtt
    12661 taaaagtaaa tccattacga cgaaacacgg atgtagactg tgacatgaaa ataaaacctt
    12721 gcaggaaaaa cggcgatagc accgggtgag aacaataccc gagaagcgat atccatacaa
    12781 acagatgaag attgcagccg taacgtctat aatcagcgcc ccacacagag gtagaacatg
    12841 actgtaacga ctttttccga acttgaactc gacgaaagcc tgctggaagc cctccaggat
    12901 aaaggtttca ctcgcccgac cgccattcag gctgccgcca ttccgcctgc gctcgatggc
    12961 cgtgatgtac tcggttctgc gccgacaggc accggtaaaa cggcggcgta tctgctgcca
    13021 gcgttgcagc acctgctcga tttcccacgt aagaaatccg gtccgccgcg tattttgatc
    13081 ctcaccccaa ctcgcgagct ggcgatgcag gtggccgatc atgcccgcga actggcgaaa
    13141 catacgcatc tggatattgc caccatcacc ggcggcgtgg cctatatgaa ccacgcagaa
    13201 gtgttcagcg aaaaccagga cattgtggtc gccacgaccg gacgtctgct gcaatacata
    13261 aaagaagaga acttcgattg ccgcgcggtt gaaacgctga tcctcgacga agcagaccgt
    13321 atgctggata tgggcttcgc ccaggatatc gaacatattg ctggcgaaac gcgctggcgt
    13381 aaacagaccc tgctcttttc ggcaacgctg gaaggcgatg cgattcagga ctttgccgag
    13441 cgtctgttgg aagatccggt ggaagtttct gccaatccgt ccacccgtga gcgcaaaaaa
    13501 attcatcagt ggtattaccg cgccgatgat cttgagcata aaaccgcgtt gctggtgcat
    13561 ctgttaaaac agccggaagc gacccgctca attgtgtttg tgcgtaagcg tgagcgtgtg
    13621 catgagctgg caaactggct gcgcgaagca ggcatcaaca actgctatct cgaaggtgag
    13681 atggtacaag gcaagcgtaa cgaagcgatt aaacgcttga ccgaaggccg cgtaaacgta
    13741 ctggttgcta ccgatgttgc cgcgcgcggt atcgacattc ctgacgtcag ccacgtcttt
    13801 aacttcgata tgccgcgcag tggcgatact tatttgcacc gtatcggacg taccgcgcgc
    13861 gccggtcgta aaggcaccgc gatttcgctg gtggaagccc atgaccatct gctgctgggc
    13921 aaagttggcc gttatattga agagccaatt aaagctcgcg taattgatga gttacgtccg
    13981 aaaacgcgtg cgccaagcga gaagcagacc ggcaagccat cgaagaaagt gttggctaaa
    14041 cgtgccgaga agaaaaaagc taaagagaaa gagaagccgc gggtgaaaaa acgccatcgc
    14101 gacactaaaa atattggtaa gcgccgtaaa ccaagcggaa cgggcgtgcc accgcaaacg
    14161 acagaagagt aatctcaatg ccgggtttaa gcccggcatt gtttttatcc cggcaaaaaa
    14221 cactcttcaa cacactgaat aaaagtgtgc atcgccgggc taaccgcttt tccggcatgg
    14281 tgagcgcaca ttgccgtaat ggtctgcgac tgttcgccaa aaggcaattc aattaattct
    14341 ccgcactcca actcttttac taccgcaaaa cgcggcaggt aactgactcc gatatttgcc
    14401 gcgacacaac gcttgatgct ttcgatactt ataagctcaa tggtgttttc caccgtgatc
    14461 cgccgctggc gcagcgtgct ctcaaatatc tgccggaaga cacattgtgg ttcgttgata
    14521 ataaagctac aggcgttatg tcttcccggc tcagtaaaat cgacatctgc aatttgcggt
    14581 gaagccacca gcaccagcgg ttgttcaccc aactctcgtc ggttcagagc atcatcattc
    14641 cctacgcgat aaaaaacccc gacatcggcc tcatcattca gtagcgcatc acggatcacg
    14701 taacagttca gcgactgcaa cgataaacgc acttttggag cgcgttgccg aaaacgctgc
    14761 aacacctgcg gcattcgata agagagcaac gtttcgcctg aaataacgcg tagttccccg
    14821 tccggatccg actctttttt agcagcctca cgaagcgtat ccatcactcg ggtaagttca
    14881 tagatgtgcg gcagcagctt ttttccttcg cgggtaaggc acattcgtcg accaattttc
    14941 tcaaacaatt gtaccgagaa ctcctgctca agctgctgaa tatgaaaagt caccgtcgat
    15001 tgtgtacagc acaatttttg cgaagctcgc aaaaaggaac cctcttccac cacggtttta
    15061 agcgtaataa aacggcgcag atccataacc ccaaacctat cgaaaatatc gaatttagaa
    15121 tgtaaaaaca ttcatttttt taaatgttcc gtgtcgggta ctgtctacca aaacagagga
    15181 gataacaagt gacaccgacc cttttaagtg ctttttggac ttacaccctg attaccgcca
    15241 tgacgccagg accgaacaat attctcgccc ttagctctgc tacgtcgcat ggatttcgtc
    15301 aaagtacccg tgtgctggca gggatgagtc tgggattttt gattgtgatg ttactgtgtg
    15361 cgggcatttc attttcactg gcagtgattg acccggcagc ggtacacctt ttgagttggg
    15421 cgggggcggc atatattgtc tggctggcgt ggaaaatcgc caccagccca acaaaggaag
    15481 acggacttca ggcaaaacca atcagctttt gggccagctt tgctttgcag tttgtgaacg
    15541 tcaaaatcat tttgtacggt gttacggcac tgtcgacgtt tgttctgccg caaacacagg
    15601 cgctaagctg ggtagttggc gtcagcgttt tgctggcgat gattgggacg tttggcaatg
    15661 tgtgctgggc gctggcgggg catctgtttc agcgattgtt tcgccagtat ggtcgccagt
    15721 taaatatcgt gcttgccctg ttgctggtct attgcgcggt acgcattttc tattaacgaa
    15781 aaaaagcgga agaggtcgcc ctcttccgct tagtaacttg ctacttaagc cttacaggct
    15841 ttcagtaaag gtacgagcga taacgtcgcg ctgctgttcc ggagtcagag agttaaagcg
    15901 aactgcataa ccggatacac ggatggtcag ctgcggatat ttttccggat gcttaactgc
    15961 atcttccaga gtttcacgac gcagaacgtt aacgttcagg tgttgaccac cttcaacgcg
    16021 aacttctggt ttcacttcta ctggaacttc acggtattca atgtcaccca gtttgcttac
    16081 tgcaaccact tcgtcttctg caaaacctgc tttcgcaacg atgcaacgcg cttcgccttt
    16141 ttcgctgtcc agcaaccaga aagagttcag cagatcgtcg ttagcggctt tagtaatctg
    16201 gatacctgta atcatgtgat gcctccccgg caaaattatt tgatttgttc agcctgtcgc
    16261 ggccaattgg taaaaccatt gttgcttgag tgtatatata ctcctcaaac acccttgatt
    16321 ctttgattta aatc
//
LOCUS       DACADF010000108        16203 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.110, whole genome shotgun sequence.
ACCESSION   DACADF010000108 DACADF010000000
VERSION     DACADF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.110"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(128..1411)
                     /locus_tag="ISB30_003331"
     CDS             complement(128..1411)
                     /locus_tag="ISB30_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HAP1990932.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1524..3458)
                     /gene="yeaG"
                     /locus_tag="ISB30_003332"
     CDS             complement(1524..3458)
                     /gene="yeaG"
                     /locus_tag="ISB30_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAP1990933.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3894..4640
                     /locus_tag="ISB30_003333"
     CDS             3894..4640
                     /locus_tag="ISB30_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAP1990934.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4730..5584
                     /gene="yeaE"
                     /locus_tag="ISB30_003334"
     CDS             4730..5584
                     /gene="yeaE"
                     /locus_tag="ISB30_003334"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HAP1990935.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRENVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5635..6519)
                     /locus_tag="ISB30_003335"
     CDS             complement(5635..6519)
                     /locus_tag="ISB30_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAP1990936.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGIPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6603..7598)
                     /gene="gapA"
                     /locus_tag="ISB30_003336"
     CDS             complement(6603..7598)
                     /gene="gapA"
                     /locus_tag="ISB30_003336"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP1990937.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7940..8353
                     /gene="msrB"
                     /locus_tag="ISB30_003337"
     CDS             7940..8353
                     /gene="msrB"
                     /locus_tag="ISB30_003337"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP1990938.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8395..8667
                     /locus_tag="ISB30_003338"
     CDS             8395..8667
                     /locus_tag="ISB30_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAP1990939.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            9037..10113
                     /locus_tag="ISB30_003339"
     CDS             9037..10113
                     /locus_tag="ISB30_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAP1990940.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVTVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10140..11519
                     /locus_tag="ISB30_003340"
     CDS             10140..11519
                     /locus_tag="ISB30_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1990941.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11536..12579
                     /locus_tag="ISB30_003341"
     CDS             11536..12579
                     /locus_tag="ISB30_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HAP1990942.1"
                     /translation="MKNSKAMLQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12600..13436
                     /locus_tag="ISB30_003342"
     CDS             12600..13436
                     /locus_tag="ISB30_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP1990943.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAIKERALDKIKLFGSDGK
                     AE"
     gene            13441..14388
                     /locus_tag="ISB30_003343"
     CDS             13441..14388
                     /locus_tag="ISB30_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAP1990944.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KTRQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            14398..15378
                     /gene="ydjG"
                     /locus_tag="ISB30_003344"
     CDS             14398..15378
                     /gene="ydjG"
                     /locus_tag="ISB30_003344"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAP1990945.1"
                     /translation="MKKIPLGTADITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            15515..>16203
                     /locus_tag="ISB30_003345"
     CDS             15515..>16203
                     /locus_tag="ISB30_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1990946.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFVDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYI"
BASE COUNT         4198 a         3648 c         4077 g         4280 t
ORIGIN      
        1 ctggaactaa attgtgtaaa ggttatgctg tcatatttaa agcgattgta agctaatgta
       61 tgtaataaaa gagataattt acagtgagct aaaaatcagc caggcgataa tgacccggct
      121 gacagtttta gtctttagct gttgcatttt gtttatgaaa cagttcacgg aacaccggat
      181 aaatatcatc ctggtcgcgg atatgctgca tcgcaaagtt gtcgaaagta gattgcagat
      241 gctcatattc tcgccacagt gtctgatgcg cgcgacgtgt aatttcgata tagctgtaat
      301 aacgaacaac gggtaatatt tttttcgcca gaatttcatg gcaaagcgga gagtcatcgg
      361 cccagttatc gccgtctgat gcttgggctg cgtaaatatt ccactgtgcc gggttataac
      421 gctctttcac tacctcatcc atcagtttta gggcgctgga aacaatggtg ccgccagttt
      481 cctgcgagta gaaaaactca tgttcatcga cttctttcgc ctgggtatga tggcggatgt
      541 atacgacttc cacgttctta tacgttctgc tgaggaacag atacagcaga atataaaaac
      601 gtttagccat atctttagtg gactgatcca ttgaaccgga aacgtccatc aggcaaaaca
      661 tcactgcctg gctggaggga tcgggccgtt tttcgtagtt cttgtaacgt aaatcgaagg
      721 tgtcaatgaa agggacgcgt tcaattttgg cacgtaattc tgcaatttct ttgcgcagac
      781 gttcctcttc cagcagttgc gcaggttcac tgttgctgat gatggccaaa ttctcttcca
      841 gcgcatgaag ttcccgccgc ttgcccgccg tcatagctgt gcgccgcgcc agtgagttct
      901 gcaatgaacg tacaacgctg atattggccg gaacgccgtt agcggtataa cccgcccgat
      961 gcgttttata ttcggtcagc tggcgttgtt ggttttgttt cagattcggt aaggccaaat
     1021 cttcaaagag cagatcaaga tactcatctt tcgaaatctg aaagacaaat tcatcctgac
     1081 cttcaccatc ctggctggcc tggccctgac cactgccgga acctccgcca ccaccctggg
     1141 gacgttcaat tcggtcgttc tggacgaaat ggtcattgcc cggatgcaca cggtggcgca
     1201 ggcccccacg cccctgatga aacatcggtt cgctaatatc ttccgtggga atggatacgg
     1261 actcgccgct gtcgacgtca gtcaccgaac gcttattaat ggcctcggag atcgactgtt
     1321 taatttgcgc tttataacgg cgtaagaaac gctggcgatt caccatgctt ttatttttgc
     1381 cgttcagacg ccggtcaata aaccaggtca tatgcccccc gtactacatt tgccaacggt
     1441 atgctacgct tgtgttgccg cttctctggc ccacggttcg ctgtcgttgt aggcccggta
     1501 gcatgtgtac cgggccaggg ttgttaagac gatttacgta cgcgcaaata ccattcgcac
     1561 agtaaacgca cctgtttacg ggtgtagcct ttttccatca tacggtcgac aaagtcgtcg
     1621 tgtttcttct gctcgtcggt tgacgttttg gcgttaaacg aaataaccgg caacagctct
     1681 tcggtattgg agaacatttt cttctcgata accgtgcgca gtttttcata gctggtccag
     1741 ttcggattgc gtccgctgtt attcgctctg gctcgcagta cgaagttaac aatctcgttg
     1801 cggaaatctt ttggattact gatccccgcc ggtttctcga ttttctccag ctcggcgtta
     1861 agagactcgc ggtcaaacag ctgcccggta tccggatcgc gatactcctg atcctgaatc
     1921 cagaaatccg cgtaggtaac ataacggtcg aaaatgtttt gcccatattc tgaataggat
     1981 tcaaggtagg ccgtctggat ctctttgccg ataaactcgg catatttcgg gatcagataa
     2041 cctttcagga actccagata gcgttcagcc tgctcttgcg ggaactgctc gcgctcgatc
     2101 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
     2161 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
     2221 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttgggatc agtgtctttc
     2281 agactttcac catcataaac gcgcatcttt gaataaatgc tggagttttc tggctctttc
     2341 agacgcgaaa gaatagaaaa acgtgacagc gtttccagcg tgccaggggc gcagggggcg
     2401 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
     2461 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
     2521 ttacggaaag tgacccattc ggattcgttc gagtgggcga gaataatccc gttgaacggc
     2581 agggcggaga taccttccgt cccgttgtag ttaccttcct gggtggcggt taacaaggga
     2641 tgcagcactt ttatcggtgc tttaaacatc tcgacaaatt ccatgatccc ctgattggcg
     2701 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
     2761 ttacgaatat cgactttccc taccagggcg gagatgtcct ggttgttctc atctccgggt
     2821 tccgttttgg cgatggcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
     2881 ttagtgatat cgccaccaaa ttcatgcagg cgctttgccg cccacggcga catgatggtg
     2941 ccgagataac ggcgaggaat gccgtactct ttttccagaa tctgcgcatc ttcctgcgga
     3001 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
     3061 taaatcggta cgagctgcat taatgatttc agtcgctcag caagtgatga tttaccccca
     3121 cccacaggcc ccagcagata cagaatttgt ttcttctctt ccagcccctg agccgcgtgt
     3181 ttcagataag aaacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaacgcc
     3241 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctgggttc ctgggctgta
     3301 tcgaccatga caggctcacc aatagccatc aatagccgct cagccgcgtt ggcataagca
     3361 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     3421 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     3481 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     3541 cgacgtctct tgctaaaaca cagcaactct catgccattt tattgacgat gagaacaacg
     3601 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     3661 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     3721 aataataggg tcgccggtaa cgctaaattc atttggctgt aagcgtggtg tcatctgcgt
     3781 caggaaaatt aaacagttac tataaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     3841 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     3901 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     3961 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga acacccatat aaagattacg
     4021 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     4081 gcttaggtgg tggttactac ctgtggaatg acgcgacgga caaactttca attaccgctt
     4141 actggtcgcc gctttacttc aaagctaaag acagtggcga tcaccaaatg cgtcacctcg
     4201 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     4261 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatc gtctgggata
     4321 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     4381 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     4441 gcagcggtct gcgtggctat aacccgaacg acagctggag cccttacctg gagctgagcg
     4501 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     4561 ctgatgaagt tactgacagc ccaatggtgg ataaatcctg gactggcctg atttctaccg
     4621 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     4681 cgtaatgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     4741 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     4801 gggcgaagat gccagtcagc gtaaaacaga agttgctgca ctacgcgcgg gcattgaact
     4861 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaagtggt
     4921 tggggaagca ttaatcggtc tgcgtgagaa cgtctttctc gtctctaaag tctatccgtg
     4981 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     5041 tgattatctc gatctctact tattacactg gtctggcagt ttcgctctcg aagagactgt
     5101 cgcagcgatg gaaaaattga tcgcccaggg aaaaatccgc cgctggggcg tttctaacct
     5161 tgattatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     5221 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctacttc cctggtgcca
     5281 gaaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     5341 tggactgtta aaaaatgcgg tagtcaacga aattgcacat gctcacaata tcagtgcggc
     5401 acaagtattg ttggcgtggg taatcagcca tcagggtgtg atggcgattc caaaagcggc
     5461 aactgtcgca catgtccaac aaaatgcggc tgtgcttgag gttgaacttt cgtcagcgga
     5521 attagttatg ctggataaag cgtatccggc accaaaagga aaaactgcgc tggatatggt
     5581 gtgatgttga ttcgtgccgg atgcatcgca cccggcacac aatattaacg caaattaacg
     5641 tttagcaacg cgaatggatt gcgccagatg tgcaggtttc tctttggtga ctttttgcgt
     5701 ttctgaagcg taagccgttt ctacacaaac aaaggtcttg tagccatcat ccggcatatc
     5761 gcccatgcta actgaaagcg ccggtcctgg gttccagcct acaacgttca gatgatgctg
     5821 gtggcctacg gcgataatac gattcagcgc ttcgtcatta attacgctgc aatcttgcgg
     5881 attcagatac acgcgatcgg tacggtcagg gaaggtctga ataccgtcgg tcagtacatc
     5941 ttctttcgcg tcatttactt tatcaatgaa gcgatcgcct agtccactga cgcttacctt
     6001 agcgatatca cccacgttaa agtaggtatg cagagcagag gtagtttcaa attcgccgtg
     6061 tgcttcaaga tcgatttcac aggttttacc cacgtggaaa tgcgctaaca gcgtaaagtc
     6121 gtgcggccag aattttttcg tctcttcgct ttgtgtcaat tcaaaagtca gcgctacacc
     6181 atcagcatct tcacgatgtg atttcagcgt ccacggcagg ttgcgggcaa aaccgtgcgc
     6241 aggcagacct tgttgtgcag ccggaccaaa ccacggccag caaaccggta tgccaccgcg
     6301 aatggcgacg ccatttttga acggtgtgtt gttactcaac cacagaactt cttcttcacc
     6361 cgcaggtttc cacgagagaa ggtgtgcgcc ctgtaatgca aaagaggctt ttacctgggg
     6421 atgatcgacc acaatgaggt ccagttcatc cagtttacga cgggagagga caggggagat
     6481 ttgttcgatg accggaaggg caaaaatttt cttaatcatg acgcagtcct ttaacttcat
     6541 tttatcaggt aaaaaaaaga gcgaccgaag tcgctctttt tagatcacag tgtcatctca
     6601 acttatttgg agatgtgagc gatcaggtcc agaactttgt tggagtaacc ggtttcgttg
     6661 tcgtaccagg ataccagttt cacgaagttg tcgttcagag cgatacccgc tttagcatcg
     6721 aacacggaag tgcaaacttc gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag
     6781 cccagaacgc ctttcatttc gccttcagca gcagctttaa cggcagcttt gatctgctcg
     6841 taagttgcag ctttttccag acgaacggtc aggtcaacta cggatacgtt cggggtcgga
     6901 acgcggaacg ccataccagt cagtttgcca ttcagttctg gcagtacttt acctacagct
     6961 ttagcagcac cggtagaaga cgggatgatg ttctgggaag cgccgcggcc gccgcgccag
     7021 tctttgtgag acgggccatc aacggttttc tgagtagcgg tagtagcgtg aacggtggtc
     7081 atcagacctt cgatgatgcc gaagttatcg ttgataactt tagccagcgg agccaggcag
     7141 ttggtggtgc aggatgcgtt ggaaacgatg tcctggccag catatttgtc gaagttagcg
     7201 cctttaacga acatcggagt gttgtcttta gacggaccag tcataaccac tttcttcgca
     7261 ccagcggtga tgtgtttacg agcagtttcg tcagtcagga acagaccagt tgcttcagcg
     7321 acaacgtcaa caccaacttc gtcccatttc aggttagccg gatcacgttc agcggtaaca
     7381 cggatttttt taccgttaac gatcagatga ccgtctttca cttcaacggt accgtcgaaa
     7441 cggccgtgag tggagtcata tttcagcatg tatgccatgt aatcagcgtc taacaggtcg
     7501 ttgattgcaa cgatctcgat gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg
     7561 atacggccaa aaccgttgat acctactttg atagtcatat attccaccag ctatttgtta
     7621 gtgaataaaa ggttgcctgt aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg
     7681 tcaatcattg cgacaaatca atcctgtgcc taagcattac gcgactgact cgcctcactc
     7741 ttcctttggg cttgagacca catggggtcg gcgccccgaa ttttaaaggg caattacgat
     7801 aaaaatgtga ttttcatcac gatttaggtg cagccatttc gtcgagatca agtttaggac
     7861 aaaattttgc cagttggtgt taatgttttg ttagaatcag tcacgtaatg tgagcacgat
     7921 taaagtgaga tgtgagcaaa tggctaataa accttcggca gaagaactga aaaaaaattt
     7981 gtccgagatg cagttctacg tgacgcagaa tcatgggaca gaaccgccat ttacgggtcg
     8041 tttactgcat aacaagcgtg acggcgtata tcactgtttg atctgcgatg ccccgctgtt
     8101 tcattcccaa accaagtatg attccggctg tggctggccc agtttctacg aaccggtgag
     8161 tgaagaatct attcgttata tcaaagactt gtcacatgga atgcagcgca tagaaattcg
     8221 ttgcggtaac tgcgatgccc atctgggcca tgtctttcct gacgggccgc agccaacggg
     8281 cgaacgttat tgtgttaact ctgcttcttt acgcttcact gatggcgaaa acggcgaaga
     8341 aatcaacggt tgaaaaaacg attcagcaac ttattccaca ggagcggaaa aattatgaat
     8401 cttgatgaca ttatcaacag catgacgcct gaggtatacc agcgtttgtc gaccgccgtt
     8461 gaactgggga aatggcctga tggcgttacg ttgaccgagg aacaaaaaga gaactgcctg
     8521 caactggtga tgctgtggca agcccgccat aataccgaag cacagcatat gacaattgac
     8581 acaaatggtc agatggtcat gaagagcaaa cagcagttaa aagaagattt tggtatctca
     8641 gcaaagccta ttgccatgtt taagtaagtg gtttatcccc tttttgccgc ggttataaag
     8701 gggatagcca acaatgaccc cacgactcat ctcaattatc tcgccccgac ctggctaagt
     8761 cccagtccga tctcttgcag tcgcgttcac acctgaatga cttctcctga acttaagtct
     8821 caggcctatt tggccgggaa tccctctcga atttgctctt tttgcgtccc gccttccatc
     8881 aacctgtaac aaccccagcc attttctgcg taattgtgat ctaagttgta aaacgcagat
     8941 cattatctgt gttttacaga tttaatgttg acctcaaaaa tcatgtgggt aagtatgatg
     9001 accatctaag caaaacaaaa cacagaggat gacgcaatga aagcactggc tcggtttggc
     9061 aaggcttttg gtggctacaa gatgattgat gtcccacaac ccatttgtgg cccggaagat
     9121 gtcgtgattg aaattaaagc cgcggcaatc tgcggcgcag acatgaagca ctacaatgtc
     9181 gatagcggtt cggatgagtt taactctatc cgcggccatg agttcgcagg ttgtattgcg
     9241 caggttggtg aaaaagtcaa agactggaaa gtggggcaac gcgtcgtgtc ggataacagc
     9301 ggtcacgttt gcggcgtttg cccggcctgt gaacaaggtg attttctgtg ttgtacagaa
     9361 aaagtaaacc ttggtctgga taacaatacc tggggcggtg gtttttccaa atattgtctt
     9421 gttcctggtg aaattctcaa aattcatcgt catgcgttgt gggaaatccc tgatggtgtt
     9481 gattatgagg acgcagccgt acttgaccct atctgtaatg cctataaatc catcgcgcag
     9541 caatcgaaat tccttccagg tcaggatgtg gtcgtcatcg gcactggccc actcgggctg
     9601 ttctccgtac aaatggcgcg gattatgggg gcggtaaata tcgtcgtcgt tggtctgcaa
     9661 gaagatgtaa cggtccgctt cccggttgca aaagaactgg gtgcgacggc agtagtaaat
     9721 ggttctaccg aagatgtggt ggcacgctgc cagcaaattt gtggcaaaga caatctgggg
     9781 ctggtgattg aatgctccgg tgccaatatc gcattgaaac aagccatcga aatgctccgc
     9841 ccgaatgggg aagtagtacg cgttggaatg ggcttcaaac ctcttgattt ctcgattaat
     9901 gacattaccg cctggaacaa aagcatcatt gggcatatgg cctatgactc cacttcatgg
     9961 cgtaacgcta tcaggctatt agccagcggc gctatcaaag tcaaaccgat gatcacgcat
    10021 cgtatcggcc tgtcgcaatg gcgcgaaggg tttgatgcga tggtcgataa aactgcaatc
    10081 aaagtgatca tgacttacga ctttgatgaa taactactct cctgaaggaa cgttatcgca
    10141 tggaacagat aacaaaaccg cattgtggtg cacggctgga tcgcttaccg gattgccgct
    10201 ggcattcgtc gatgtttgcc atcgtcgcgt ttggcctgct tgtctgctgg agtaatgccg
    10261 ttggtggctt gatcctcgcg cagctgaaag cgttgggctg gacagataat tccaccactg
    10321 ccacattctc agcaatcacg accgccggaa tgtttctcgg tgcgctggtg ggcggcatca
    10381 tcggtgacaa aaccggtcgc agaaatgcgt ttatcctcta tgaggccatt catattgcct
    10441 cgatggttgt cggtgctttc tcaccgaata tggatttcct tattgcctgc cgttttgtga
    10501 tgggcgttgg gctgggagct ttactggtta cactgtttgc tggtttcacc gaatatatgc
    10561 ccggaagaaa tcgcggaacg tggtccagtc gggtttcctt tattggtaac tggtcatatc
    10621 cgctctgttc attgattgcg atgggactca cgccgctgat tagtgcagag tggaactggc
    10681 gggtacaact gcttatccct gcaatattgt cacttatcgc tacggcgctg gcctggcgct
    10741 actttcctga atccccgcgc tggctggaat cgcgcggacg ttatcaggaa gccgagaaag
    10801 tcatgcggag tatagaagaa ggcgtcatac gccagacggg taaacctttg ccgcctgtgg
    10861 ttattgctga tgacggtaaa gcaccacaag cggtgccgta ttcagcctta ctgacaggag
    10921 tattactgaa acgcgtgata ttaggctctt gtgtgctgat tgccatgaac gttgtgcagt
    10981 acacactaat taactggttg ccaacgatat tcatgaccca gggaattaat ttaaaagact
    11041 cgattgtttt aaataccatg agtatgtttg gcgcgccatt tggtattttt attgccatgc
    11101 tggtgatgga taaaattccg cgtaaaacaa tgggtgtggg gctattaatc ctgattgcgg
    11161 tgctcggata tatctattca ctgcaaacca gtatgttgct cataacgctg attggtttct
    11221 tcctgattac tttcgtctat atgtacgttt gctatgcctc ggcagtgtat gtccctgaga
    11281 tctggccgac agaggccaaa ctccgtggct ccggtctggc gaatgcggta gggcgaatca
    11341 gtggtattgc cgcaccttat gccgttgcag tgctgctcag tagttatggc gtaacgggag
    11401 tctttattct tctgggggct gtttcaatta ttgtcgcaat tgctatcgcc accattggaa
    11461 ttgaaaccaa aggtgtctcc gttgaaagtt taagtattga tgcagtagcc aataaataat
    11521 actggtggat tcaaaatgaa aaattcaaaa gcaatgttgc aggtgccggg cacaatgaaa
    11581 attatttcag cagaaatacc tgtacctaaa gaagatgaag ttttgattaa agtggaatat
    11641 gtcggtattt gtggttcaga tgtacatggt tttgaatcag gcccgtttat tcctcctaaa
    11701 gacccaaatc aagaaattgg cctgggtcat gaatgcgccg ggacggttgt ggctgtggga
    11761 agccgcgtgc gcaaatttaa accgggggat cgggtaaata tcgaacctgg cgttccttgc
    11821 ggtcactgtc gttactgtct ggaaggcaaa tataacatct gcccggacgt tgattttatg
    11881 gcgacacaac ccaactaccg cggcgcatta acgcactatc tgtgtcatcc ggagagcttt
    11941 acttacaaac tgccagacaa tatggacacg atggaagggg cgctggtgga gcctgccgca
    12001 gtcgggatgc atgccgcgat gctggcagat gttaaaccgg gtaagaagat aattattctg
    12061 ggagcgggtt gtattggttt gatgacgttg caagcgtgca aatgcctggg agcaacggaa
    12121 attgccgtcg ttgatgtgct ggaaaaacgt ctgacaatgg cggaacagct cggcgcgaca
    12181 gtggttatta acggcgcaaa agaagacact attgcacgct gtcagcaatt taccgaagac
    12241 atgggcgcag atattgtttt tgaaacagcg ggttctgcgg tcaccgttaa acaggcacct
    12301 tatctggtca tgcgcggcgg taaaattatg attgttggta ctgtacccgg cgattcggca
    12361 atcaatttcc tcaaaatcaa tcgcgaagtc actatccaga cggtattccg ctatgccaat
    12421 cgttatccgg tcacgattga agctatttct tcagggcgat tcgatgtgaa atcgatggtg
    12481 acgcatattt acgattatcg ggatgtacaa caggcatttg aagagtcagt taacaacaaa
    12541 cgcgacatta ttaaaggcgt tattaaaatt agcgattaat taatacggga gaacagaaca
    12601 tgctcgcaga tatcagatat tgggaaaacg atgccacaaa taaacattat gcaattgccc
    12661 attttaacgt atggaatgca gaaatgttga tgggcgttat cgacgcagcc gaagaagcga
    12721 aatcaccggt tattatttct tttggcacag gttttgtcgg caacacctca tttgaagatt
    12781 tctctcacat gatggtatca atggcacaaa aagcaacggt gccggtaata actcattggg
    12841 atcatggtcg gagtatggag attattcata acgcctggac tcatggtatg aattcattaa
    12901 tgcgtgatgc ttctgcattt gatttcgaag aaaatattcg tttaaccaaa gaggctgttg
    12961 atttcttcca tccgctgggt attccggtag aggcggaatt agggcatgtc ggtaatgaaa
    13021 ccgtttatga ggaagcgctg gcaggctatc actataccga tcctgaccag gcggctgaat
    13081 ttgttgaacg tacgggctgt gactcattgg ctgtcgccat cggcaaccag catggggttt
    13141 atacgtcaga gccacaattg aactttgagg tcgtcaaacg cgtacgcgat gctgtttctg
    13201 ttccgctggt tttgcatggc gcatcaggga tcagtgatgc cgacattaaa actgcaattt
    13261 cgttgggtat tgcgaaaatc aacattcata cggagctctg tcaggccgca atggtagcag
    13321 tgaaagaaaa ccaggatcag cctttcctgc atttagaacg tgaagtgcgt aaagctataa
    13381 aagaacgggc attggataaa attaaactgt tcggttctga tggcaaagcg gaataataat
    13441 atggataatc tcgacgttat ttgtataggt gccgctattg ttgatattcc attgcaaccg
    13501 gtcagtaaaa atatctttga tgtggattct tatcctcttg aaagaatcgc aatgaccacc
    13561 gggggtgatg caataaatga agcaacaatt atttctcgtc tgggccatcg cacagcatta
    13621 atgagtcgta ttggtaaaga tgccgcaggg caatttattc tcgaccattg ccgcaaagag
    13681 aatattgata ttcaaagcct gaagcaggac gtcagcatag atacctctat taacgttgga
    13741 ctggtgacgg aggatggcga gcggaccttt gtcacaaacc gtaacggcag tctgtggaaa
    13801 ttgaatattg acgatgttga ttttgcgcgg ttttctcagg cgaaattatt atcactggcc
    13861 agtattttca acagtcctct attggatggt cgagcgctaa cagaaatttt tacgcaagct
    13921 aaaacccggc agatgattat ctgtgccgat atgatcaaac cgcggttgaa tgaaacgctg
    13981 aatgatattt gcgaagcatt gagttatgtc gattatctgt ttcctaattt tgccgaggca
    14041 aaattactca ccgggaaaga gacactggat gaaattgctg actgctttct tgcgtgcggc
    14101 gtaaaaacgg tggtgattaa aacgggtaaa gacggctgct ttatcaagcg tggtgacatg
    14161 acgatgaagg tgccggcggt cgcaggaata accgctatcg acaccattgg tgcgggcgat
    14221 aactttgctt caggttttat tgcagcactg ttagaaggca aaaatctgcg agaatgcgca
    14281 cgctttgcca atgcaacggc ggctatctcg gttctaagca tcggcgccac caccggtgta
    14341 aaaaacagaa agctggtcga acaattgctg gaagaatacg aaggataatg aaggcaaatg
    14401 aaaaagatac ctttaggcac agcggatata acgctttcgc gaatggggtt gggtacatgg
    14461 gctattggcg gcggtcctgc atggaatggc gatctcgatc gacaaatatg cattgatacg
    14521 attcttgaag cccatcgttg cggcattaac ctgattgata ctgcgccagg atataacttt
    14581 ggcaatagtg aagttatcgt tggtcaggcg ttaaaaaaac tgccccgtga acaggttgta
    14641 gtagaaacca aatgcggcat tgtctgggaa cgaaaaggaa gtttattcaa caaagttggc
    14701 gatcggcagt tgtataaaaa cctttctccg gaatctatcc gcgaagaggt agaagccagc
    14761 ttgcaacgtc tgggtattga ttacatcgat atctacatga cgcactggca gtcggtgccg
    14821 ccatttttta cgccgatagc tgaaactgtt gcagtgctta atgcgttaaa ggccgaaggg
    14881 aaaattcgcg ctataggcgc tgctaacgtc gatgctgacc atatccgcga gtatctgcaa
    14941 catggtgaac tggatattat tcaggcgaaa tacagtattc tcgaccgggc gatggaaaac
    15001 gaactgctgc cgctatgtcg tgataatggc attgtggttc aggtttattc cccgctagag
    15061 cagggattgt tgaccggcac catcacgcgt gattacgttc cgggcggcgc tcgggcaaat
    15121 aaagtctggt tccagcgtga aaacatgctg aaagtgattg atatgcttga acagtggcag
    15181 ccactctgtg ctcgttatca gtgcacaatt cccactctgg cactggcgtg gatattaaaa
    15241 cagagtgatt taatctccat tcttagtggg gctactgcgc cggaacaggt acgtgaaaat
    15301 gtcgcggcac tgaatatcaa cttatcggat gcagacgcaa cattgatgag ggaaatggca
    15361 gaggccctgg agcgttaaat actcaacacg attcacctct gtttctgtga aaactgtgat
    15421 gaaaatctgt gattcaaaca ggttattttg aaagtaaaca tcggttatgc gataattgcg
    15481 ctaatgtgat gtaaaaagat acaggggtgt aatcgtggcg gcaaaagaca ggattcaggc
    15541 aattaagcaa atggttgcca acgataaaaa ggtgacagtc tcaaatttga gtgggatttt
    15601 tcaggtaacc gaagaaacca ttcgccgcga tcttgagaag ttggaagatg aaggcttttt
    15661 gaccagaacc tatggtggtg ctgttttaaa tacagcgatg ttgacggaga atatccattt
    15721 ttataagcgc gcttcatcgt tttatgaaga gaagcagctt attgcacgca aggcactacc
    15781 ctttgtcgac aataaaacca ccatggcagc cgattccagt agtaccgtaa tggaattgct
    15841 caaattgtta caggaccgta gtgacctgac gttgctaacc aactcggcgg aagcgattca
    15901 tgttctggct cagtcagaaa ttaaagtcgt ttcaacgggt ggggaactaa acaaaaatac
    15961 actttcactg caaggaagaa ttactaaaga gatcatcagc cgctaccatg tcgatatcat
    16021 ggtaatgagt tgtaaaggtc ttgatattaa cagcggcgcg ctggactcta atgaagcgga
    16081 agctgaaatc aaaaagacaa tgatccgtca ggcgacagaa gttgcgttat tggttgatca
    16141 ctctaagttt gatcgcaaag cttttgtcca gttagctgac tttagtcata ttaattacat
    16201 aat
//
LOCUS       DACADF010000109        15921 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.111, whole genome shotgun sequence.
ACCESSION   DACADF010000109 DACADF010000000
VERSION     DACADF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.111"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..230)
                     /locus_tag="ISB30_003346"
     CDS             complement(<1..230)
                     /locus_tag="ISB30_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1990947.1"
                     /translation="MSENDIIIRTHYESPLRMHIDSDILTPSSEPISQFARQLITLLD
                     TSDLSSMLSYCVTQEFTVSCRKISHNCYSTAL"
     gene            754..2682
                     /gene="thrS"
                     /locus_tag="ISB30_003347"
     CDS             754..2682
                     /gene="thrS"
                     /locus_tag="ISB30_003347"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP1990948.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2686..3228
                     /gene="infC"
                     /locus_tag="ISB30_003348"
     CDS             2686..3228
                     /gene="infC"
                     /locus_tag="ISB30_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP1990949.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3325..3522
                     /gene="rpmI"
                     /locus_tag="ISB30_003349"
     CDS             3325..3522
                     /gene="rpmI"
                     /locus_tag="ISB30_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP1990950.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3575..3931
                     /gene="rplT"
                     /locus_tag="ISB30_003350"
     CDS             3575..3931
                     /gene="rplT"
                     /locus_tag="ISB30_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP1990951.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            4054..4098
                     /locus_tag="ISB30_003351"
     CDS             4054..4098
                     /locus_tag="ISB30_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAP1990952.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4382..5365
                     /gene="pheS"
                     /locus_tag="ISB30_003352"
     CDS             4382..5365
                     /gene="pheS"
                     /locus_tag="ISB30_003352"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP1990953.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5380..7767
                     /gene="pheT"
                     /locus_tag="ISB30_003353"
     CDS             5380..7767
                     /gene="pheT"
                     /locus_tag="ISB30_003353"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP1990954.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGIHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7772..8071
                     /gene="ihfA"
                     /locus_tag="ISB30_003354"
     CDS             7772..8071
                     /gene="ihfA"
                     /locus_tag="ISB30_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAP1990955.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8172..9152
                     /gene="btuC"
                     /locus_tag="ISB30_003355"
     CDS             8172..9152
                     /gene="btuC"
                     /locus_tag="ISB30_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAP1990956.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPVNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9215..9766
                     /gene="btuE"
                     /locus_tag="ISB30_003356"
     CDS             9215..9766
                     /gene="btuE"
                     /locus_tag="ISB30_003356"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAP1990957.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9766..10515
                     /gene="btuD"
                     /locus_tag="ISB30_003357"
     CDS             9766..10515
                     /gene="btuD"
                     /locus_tag="ISB30_003357"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAP1990958.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQNALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10593..11057
                     /locus_tag="ISB30_003358"
     CDS             10593..11057
                     /locus_tag="ISB30_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAP1990959.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11305..12018
                     /gene="ydiV"
                     /locus_tag="ISB30_003359"
     CDS             11305..12018
                     /gene="ydiV"
                     /locus_tag="ISB30_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HAP1990960.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12081..13517
                     /locus_tag="ISB30_003360"
     CDS             12081..13517
                     /locus_tag="ISB30_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAP1990961.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGENLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWPHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGLNEALDSYQQVLLTHYGQRMRQKLG
                     FMTELKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEITDNERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13521..13712)
                     /gene="hemP"
                     /locus_tag="ISB30_003361"
     CDS             complement(13521..13712)
                     /gene="hemP"
                     /locus_tag="ISB30_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAP1990962.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13844..14890)
                     /gene="aroH"
                     /locus_tag="ISB30_003362"
     CDS             complement(13844..14890)
                     /gene="aroH"
                     /locus_tag="ISB30_003362"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAP1990963.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(15047..15880)
                     /gene="ppsR"
                     /locus_tag="ISB30_003363"
     CDS             complement(15047..15880)
                     /gene="ppsR"
                     /locus_tag="ISB30_003363"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAP1990964.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         3981 a         3742 c         4236 g         3962 t
ORIGIN      
        1 aaaagggcag tggaataaca attgtgtgat atttttcgac agcttacggt aaattcctga
       61 gtaacacagt atgacagcat cgaacttaaa tcagaggtat caagtagggt gatgagctgg
      121 cgcgcaaatt gactaatagg ctctgatgaa ggcgttagta tgtcgctatc gatgtgcatt
      181 ctaagaggag actcataatg agttctgata atgatatcgt tttcactcat aatctattcg
      241 ccttatatat gtctggagat tttgaggatg atgattaatt attatcgcca gaacgaaatg
      301 cgcacgaaaa attgctataa gaattgtgtc acttttatca ttgcgatatg accacttcgt
      361 cactgaatct catgatcgtt taaataccac cgccaaataa cgcaacgaac tttgctggat
      421 tcagtttatg ctgctgtaaa tccgctcgta gtaaaccttt cagacactct gtgatgatat
      481 cagtggttct ttaagcgttt tgctggtgta ctcactacaa acgaattgcg aatcgaatca
      541 atgtgaaacg gaaaggtaca atctcccttt ttttagtgtt aacatcgttc aaccgggtgg
      601 aaggtgagag gttaatgtct ttcatttgtg tacctgattg cgaaccaatt tagcatttgt
      661 tggctagatg gtttcgcaat gaactgttaa taaacaaatt tttctttgta tgtgatcttt
      721 cgtgtgggtc accactgcaa ataaggatat ataatgcctg ttataactct tcctgatggc
      781 agccaacgcc attacgacca cgctgtaagc cccatggatg ttgcgctgga cattggtcca
      841 ggtctggcga aagcctgtat cgcagggcgc gttaatggcg aactggttga tgcttgcgat
      901 ctgattgaaa acgacgcaca actgtcgatc attaccgcca aagacgaaga aggtctggag
      961 atcattcgtc actcctgtgc gcacctgtta gggcacgcga ttaaacaact ttggccgcat
     1021 accaaaatgg caatcggccc ggttattgac aacggttttt attacgacgt tgatcttgac
     1081 cgcacgttaa cccaggaaga tgtcgaagca ctcgagaagc ggatgcatga gcttgctgag
     1141 aaaaactacg acgtcattaa gaagaaagtc agctggcatg aagcgcgtga aactttcgcc
     1201 aaccgtgggg agagctacaa agtctccatt cttgacgaaa acatcgccca tgatgacaag
     1261 ccaggtctgt acttccatga agaatatgtc gatatgtgcc gcggtccgca cgtaccgaac
     1321 atgcgtttct gccatcattt caaactaatg aaaacggcag gggcttactg gcgtggcgac
     1381 agcaacaaca aaatgttgca acgtatttac ggtacggcgt gggcagacaa aaaagcgctt
     1441 aacgcttacc tgcaacgcct ggaagaagcc gcgaaacgcg accaccgtaa aatcggtaaa
     1501 cagctcgacc tgtaccatat gcaggaagaa gcgccgggta tggtattctg gcacaacgac
     1561 ggctggacca tcttccgtga actggaagtg tttgttcgtt ctaaactgaa agagtaccag
     1621 tatcaggaag ttaaaggtcc gttcatgatg gaccgtgtcc tgtgggaaaa aaccggtcac
     1681 tgggacaact acaaagatgc aatgttcacc acgtcttctg agaaccgtga atactgcatt
     1741 aagccgatga actgcccggg gcacgtacaa attttcaacc aggggctgaa gtcttatcgc
     1801 gatctgccgc tgcgtatggc cgagtttggt agctgccacc gtaacgagcc gtcaggttcg
     1861 ctgcatggcc tgatgcgcgt gcgtggtttt acccaggatg acgcgcatat cttctgtact
     1921 gaagaacaaa ttcgcgatga agttaacgga tgtatccgtt tagtctatga tatgtacagc
     1981 acttttggct tcgagaagat cgtcgtcaaa ctctccactc gtcctgaaaa acgtattggc
     2041 agcgacgaaa tgtgggatcg tgctgaggcg gacctggcgg ttgcgctgga agaaaacaac
     2101 atcccgtttg aatatcaact gggtgaaggc gctttctacg gtccgaaaat tgaatttacc
     2161 ctgtatgact gcctcgatcg tgcatggcag tgcggtacag tacagctgga cttctccttg
     2221 ccgtctcgtc tgagcgcctc ctatgtgggc gaagacaacg agcgtaaggt tccggtaatg
     2281 attcaccgcg caattctggg gtcgatggaa cgtttcatcg gtatcctgac cgaagaattc
     2341 gctggtttct tcccgacctg gcttgcgccg gttcaggttg ttatcatgaa tattaccgat
     2401 tcacagtctg attacgttaa cgaattgacg caaaaactat caaatgcggg cattcgtgtt
     2461 aaagcagact tgagaaatga gaagattggc tttaaaatcc gcgagcacac tttgcgtcgc
     2521 gtcccatata tgctggtctg tggtgataaa gaggtggaat caggcaaagt tgccgttcgc
     2581 acccgccgtg gtaaagacct gggaagcatg gacgtaaatg aagtgatcga gaagctgcaa
     2641 caagagattc gcagccgcag tcttaaacaa ttggaggaat aaggtattaa aggcggaaaa
     2701 cgagttcaaa cggcgcgccc taaccgtatc aatggcgaaa ttcgcgccca ggaagttcgc
     2761 ttaacaggtc tggaaggcga gcagcttggt attgtgagtc tgagagaagc tctggagaaa
     2821 gcagaagaag ccggagtaga cttagtcgag atcagcccta acgccgagcc gccggtttgt
     2881 cgtataatgg attacggcaa attcctctat gaaaagagta agtcttctaa ggaacagaag
     2941 aaaaagcaaa aagttatcca ggttaaggaa attaaattcc gtcctggtac agatgaaggc
     3001 gactatcagg taaaactccg cagcctgatt cgctttctcg aagagggtga taaagccaaa
     3061 atcacgctgc gtttccgcgg tcgtgagatg gcgcaccagc aaatcggtat ggaagtgctt
     3121 aatcgcgtga aagacgattt gcaagaactg gcagtggtcg aatccttccc aacgaagatc
     3181 gaaggccgcc agatgatcat ggtgctcgct cctaagaaga aacagtaagg ccttcaagta
     3241 gcactgtctg tggagccttt gggcttcaca ggcattgttc gcctttgttt cgtttattaa
     3301 caatgcgaag tggaagttat taaaatgcca aaaattaaga ccgtacgcgg tgctgctaag
     3361 cgcttcaaaa aaaccggtaa aggtggtttt aagcacaagc acgctaacct gcgtcacatt
     3421 ctgaccaaaa aagcgaccaa acgtaaacgt cacctgcgtc cgaaagccat ggtttccaaa
     3481 ggcgatctgg gcctggtaat cgcgtgcctg ccgtacgcat aagccgttaa cgtttttaac
     3541 tttttaatta gaatatagat acaggagagc acatatggct cgcgtaaaac gtggtgttat
     3601 tgcacgcgca cgtcacaaga aaattttgaa acaagctaaa ggctactacg gtgcgcgttc
     3661 tcgcgtatac cgcgttgcct tccaggctgt tatcaaagct ggtcagtatg cttaccgcga
     3721 ccgtcgtcaa cgtaagcgtc agttccgtca actgtggatt gcgcgtatca acgcagcagc
     3781 acgtcagaat ggtatttctt acagcaaatt catcaatggc ctgaaaaaag cctctgttga
     3841 aatcgaccgt aagatcctgg ctgatatcgc agtattcgac aaagtagcgt tcaccgctct
     3901 ggttgaaaaa gcgaaagcag ctctggcata agccagttga aagagggagc tagtctccct
     3961 cttttcgttt caacgccatc aaaacattga cttttatcgc cgtagccttt tcaataaagg
     4021 tcttttgaag agtaccaaaa ggtaacgcaa gcaatgaatg ctgctatttt ccgcttcttt
     4081 ttttacttta gcacctgact ccaggaggct agcgcgtgag aagagaaacg gaaaacagcg
     4141 cctgaaagcc tcccagtgga ggcttttttt gtatgcgcgt tttgaaaaat aaaaagcctg
     4201 tctcatcagg cgtaattgtc gcagccagtc tggacacggc cagcgcggag aaaccggacc
     4261 gtacttaagt acgtgaggat ttcgagcact gcccgggacc aaaatggcaa gtaaaatagc
     4321 ctgatgggat gggctctaag tccaacgaac cagtgtcacc actgacacaa tgaggaaaaa
     4381 catgtcacat ctcgcagaac tggttgccag tgcgaaggcg gccattagcc aggcgtcaga
     4441 tgttgccgcg ttagataatg tgcgcgtcga atatttgggt aaaaaagggc acttaaccct
     4501 tcagatgacg accctgcgtg agctgccgcc agaagagcgt ccggcagctg gtgcggttat
     4561 caatgaagcg aaagagcagg ttcagcaggc gctgaatgcg cgtaaagcgg aactggaaag
     4621 cgcagcactg aatgcgcgtc tggcggcgga aacgattgat gtctccctgc ctggtcgtcg
     4681 cattgaaaac ggcgggctgc atccggttac ccgtaccatc gaccgtatcg aaagtttctt
     4741 cggtgagctt ggctttaccg tggcaaccgg gccggaaatc gaagacgatt atcataactt
     4801 cgatgctctg aacattcctg gtcaccaccc tgcgcgcgct gaccacgaca ctttctggtt
     4861 tgacgctacc cgcctgctgc gtacccagac ctctggcgta cagatccgca ccatgaaagc
     4921 ccagcagcca ccgattcgta tcatcgcgcc tggccgcgtt tatcgtaacg actacgacca
     4981 gactcacacg ccgatgttcc atcagatgga aggtctgatt gttgatacca acatcagctt
     5041 tactaacctg aaaggcacgc tgcacgactt cctgcgtaac ttctttgagg aagatttgca
     5101 gatccgcttc cgtccttcct acttcccgtt taccgaacct tctgcagaag tggacgtcat
     5161 gggtaaaaac ggaaaatggc tggaagtact gggctgcggg atggtgcatc cgaacgtgct
     5221 gcgtaacgtt ggcatcgacc cggaagttta ctctggtttc gccttcggga tggggatgga
     5281 gcgtctgact atgttgcgct acggcgtcac cgacctgcgt tcattctttg aaaacgatct
     5341 gcgtttcctc aaacagttta aataaggcag gaatagatta tgaaattcag tgaactgtgg
     5401 ttacgcgaat gggtgaaccc ggcgattgat agcgacgcgc tggcaaatca aatcacgatg
     5461 gcgggcctgg aagttgatgg tgtagaaccg gttgccggta gcttccacgg cgtggttgtg
     5521 ggtgaagtgg ttgagtgtgc gcagcatccg aacgctgaca aactgcgtgt gacaaaagtg
     5581 aatgtcggcg gcgatcgcct gctggacatc gtctgcggtg cgccaaactg ccgtcagggc
     5641 ctgcgtgtgg cggtagcgac cattggtgct gttctgccgg gtgatttcaa aattaaagcc
     5701 gcgaaactgc gtggcgaacc gtctgaaggg atgctgtgct ccttctctga gctgggaatt
     5761 tctgacgatc ataacggcat tatcgagctg cctgcggatg cgccgattgg caccgacatc
     5821 cgcgaatacc tgaaactcga tgacaacacc atcgaaatca gcgtgacacc aaaccgtgcc
     5881 gactgcttag gtatcattgg tgttgcgcgc gacgttgccg tgttgaacca gctgccgctg
     5941 gttgaaccgg aaatcgttcc ggttggtgcg accatcgacg acacgctgcc gattgcagta
     6001 gaagcgccgg atgcctgccc gcgctatctt ggccgtgttg taaaaggcat taacgttaaa
     6061 gcgccaactc cgctgtggat gaaagaaaaa ctgcgtcgtt gcgggatccg ttctatcgat
     6121 gcagttgttg acgtcaccaa ctatgtgctg ctcgaactgg gccagccgat gcacgctttc
     6181 gataaagatc gcattgaagg cggcattgtg gtgcggatgg caaaagaggg tgaaacgctg
     6241 gtattgctcg acggtactga agcgaagctg aatgctgaca ccctggtcat cgccgaccac
     6301 aacaaggcgc tggcgatggg cggcatattt ggtggcgaac actctggcgt gaatgacgaa
     6361 acgcaaaacg ttctgctgga atgcgctttc ttcagcccgc tgtctatcac cggtcgtgct
     6421 cgtcgtcatg gcctgcatac tgatgcgtct caccgttatg agcgtggcgt tgatccggca
     6481 ctgcagcaca aagcgatgga acgtgcgacc cgtctgctga tcgacatctg cggtggtgaa
     6541 gcgggtccgg taattgatat cactaacgaa gcaacgctgc cgaagcgtgc aaccatcact
     6601 ttacgtcgta gcaaactgga tcgcctgatc ggccatcata ttgctgatga gcaggtaaca
     6661 gacattctgc gtcgtctcgg ctgcgaagtg accgaaggca aagacgagtg gcaggcagtt
     6721 gcgccgagct ggcgtttcga catggagatt gaagaagatc tggtcgaaga agtcgcgcgt
     6781 gtttacggct acaacaacat cccggatgag ccggtacagg caagcctgat tatggggatt
     6841 caccgtgaag ctgacctgtc gctcaagcgc gtaaaaacgc tgctcaacga caaaggctat
     6901 caggaagtga tcacctacag cttcgttgat ccgaaagtgc agcagatgat ccatccaggc
     6961 gttgaagcct tactgctgcc aagcccgatc tctgttgaaa tgtcagcaat gcgtctttct
     7021 ctgtggaccg gcctgctggc aaccgtggtg tacaaccaga accgtcagca gaatcgtgta
     7081 cgcattttcg aaagcggtct gcgtttcgtg ccagatactc aggcaccgtt gggtattcgt
     7141 caggatctga tgttagccgg tgtgatttgc ggtaaccgtt acgaagagca ctggaacctg
     7201 gcaaaagaga ccgttgattt ctatgatttg aaaggcgatc ttgaatccgt tctcgacctg
     7261 accggtaaac tgaatgaggt tgagttccgt gcagaagcga atccggcact gcatccgggg
     7321 caatccgcag cgatttatct gaaaggtgaa cgtattggtt ttgttggggt tgttcatcct
     7381 gaactggaac gtaaactgga tcttaacggt cgcactctgg tgttcgaact ggagtggaac
     7441 aagctcgcag accgcgtggt gcctcaggcg cgcgagattt ctcgcttccc ggcgaaccgt
     7501 cgtgacatcg cggtggtggt cgcagaaaac gttcccgcag cggatatttt atccgaatgt
     7561 aagaaagttg gcgtaaatca ggtagttggc gtaaacttat ttgacgtgta ccgcggtaag
     7621 ggtgttgcgg aggggtataa gagcctcgcc ataagcctga tcctgcaaga taccagccgt
     7681 acactcgaag aagaggagat tgccgctacc gtcgccaaat gtgtagaggc attaaaagag
     7741 cgattccagg catcattgag ggattgaacc tatggcgctt acaaaagctg aaatgtcaga
     7801 atatctgttt gataagcttg ggcttagcaa gcgggatgcc aaagaactgg ttgaactgtt
     7861 tttcgaagag atccgtcgcg ctctggaaaa cggcgaacag gtgaaactct ctggttttgg
     7921 taacttcgat ctgcgtgata agaatcaacg cccgggacgt aacccgaaaa cgggcgagga
     7981 tattcccatt acagcacggc gcgtggtgac cttcagaccc gggcagaagt taaaaagccg
     8041 ggtcgaaaac gcttcgccca aagacgagta atctgatcta actaaaaagg ccgctctgcg
     8101 gccttttttc ttttcactgt cgaagagtca ccgtaaaatc aacgccatga cacttcagca
     8161 gaacggatac catgctgaca cttgcccgcc aacaacagcg acaaaatatt cgctggttat
     8221 tatgcctgtc agttttgatg ctgctggcgc ttctcttaag cctttgcgcc ggtgaacaat
     8281 ggatctcgcc aggtgactgg tttactcctc gtggcgaact gttcgtctgg caaattcgcc
     8341 tgccacgtac gctggctgta ttgctggttg gtgctgcgtt ggctatatcc ggcgctgtaa
     8401 tgcaggcgtt gtttgaaaat cctctggcag aacctggact acttggcgtc tctaacggcg
     8461 caggcgtggg gcttatcgcc gcggtattgc ttgggcaagg gcaactcccc aactgggcgc
     8521 tggggctgtg tgcgattgct ggcgcgctta tcatcacttt aatactctta cgtttcgccc
     8581 gtcgtcatct ttcgaccagt cggttattgc tggctggcgt tgcattaggg attatctgta
     8641 gtgcactaat gacgtgggct atctactttt ccacctctgt tgatttacgt cagctgatgt
     8701 actggatgat gggcggtttt ggcggcgtag actggcggca aagctggctg atgctggcat
     8761 tgatccccgt gttgttgtgg atctgttgtc agtccaggcc ggtgaatatg ttagcacttg
     8821 gcgagatctc ggcgcggcaa ctgggtttac ccctgtggtt ctggcgcaat gtgttggtcg
     8881 cagcgaccgg ctggatggtt ggcgtcagtg tggcgctggc gggtgctatc ggctttattg
     8941 gtctggtgat cccacatatt ctccggctgt gtggtttaac cgatcatcgc gtattacttc
     9001 ccggctgcgc gctggcaggg gcgagcgcat tgctgctggc cgatattgta gcgcgcctgg
     9061 cattagctgc cgcagagctg cctattggcg tggtcaccgc aacgttgggt gcgccggtgt
     9121 ttatctggtt attgttaaaa gcaggacgtt agccgcaaaa agacggtcta tgattaaaag
     9181 ctaagatttt tactgaccac acccaggaga aacgatgcaa gattccattc tgacgaccgt
     9241 agtgaaagat atcgacggtg aagtgaccac gctggagaag tacgccggta atgtgctgtt
     9301 gattgtcaat gtcgcctcaa agtgtggctt aacgccgcaa tatgagcagt tggagaatat
     9361 tcagaaagcc tgggccgatc gtggttttgt ggtgctggga ttcccgtgca accagtttct
     9421 ggaacaagaa ccgggcagcg atgaagagat taaaacttac tgtaccacca catggggggt
     9481 gacgttcccg atgttcagta agattgaagt taatggcgaa ggacgccatc cgctgtatca
     9541 aaaattgatt gccgcagcgc cgaccgcagt cgcgccggaa gagagcggat tctatgcccg
     9601 tatggtcagc aaaggtcgtg caccgctgta ccctgatgat attttatgga attttgagaa
     9661 atttttagtt ggcagggacg gattggtcat ccagcgtttt tccccggata tgacgccgga
     9721 agatcccatt gtgatggaaa gcattaaact ggcgttggca aaataatgtc tattgtgatg
     9781 cagttacagg atgttgcgga atctacccgc ctggggccgc tttctggcga ggttcgggct
     9841 ggggagatcc tgcacctggt ggggccgaac ggcgcgggta agagtacctt gcttgcacga
     9901 atggccggaa tgaccagcgg taagggaagc attcagttcg cggggcaacc actggaagca
     9961 tggtctgcaa caaaactcgc gctgcatcgc gcctatcttt cacaacagca gacgccgccg
    10021 tttgcaatgc cggtctggca ctacctgaca ctgcatcagc acgataaaac gcgtaccgaa
    10081 ctactgaatg atgtcgcagg ggcgctggct cttgatgaca aactcggacg tagcaccaat
    10141 caactttccg gcggtgaatg gcaacgcgta cgtcttgctg cggtggtgct tcaaatcaca
    10201 ccacaagcta atcccgcagg ccaattgctg cttcttgatg agccgatgaa cagtcttgat
    10261 gttgcgcaac aaaatgcgtt agacaaaatt ctgagcgcgc tgtgtcagca aggactggcg
    10321 attgtgatga gcagtcacga tctcaaccac acattgcgtc atgcgcatcg ggcgtggttg
    10381 ctaaaaggtg gaaaaatgct ggccagtgga cgcagagaag aggtgctcac gccggcaaat
    10441 ctggcacagg cctatgggat gaattttcgc cgtctggata tcgaaggtca cagaatgctg
    10501 atttcgacca tctgatttca ttagtatctt gaaaatcggg taattacgac gctaaattaa
    10561 tgcaagaata aaaaacagag gaaccgtacg gaatgcgttt ctgccttatt ttgatcacag
    10621 cactgtttct ggccgggtgt agccaccata aagcaccgcc gccaaatgcc aggctttctg
    10681 attcgatcac cgttattgcc ggtttgaacg accagctaca aagctggcat ggcacgccgt
    10741 atcgttatgg tggcatgacg cggcgcggtg tggactgttc gggatttgtg gttgtgacga
    10801 tgcgcgatcg tttcgatttg cagctgcccc gtgaaaccaa acaacaagcc tctattggca
    10861 cgcaaattga taaagacgag ttgctgccag gtgacctggt ctttttcaaa acgggttccg
    10921 gacaaaatgg tttgcatgta ggtatttacg ataccaacaa ccaatttatc cacgcctcta
    10981 ccagcaaggg agtgatgcgt tcctcacttg ataatgtcta ttggcagaaa aatttctggc
    11041 aggcgagacg aatctagtat gtcgaaaaat ggacggcgat gatacatcgc cgtccattta
    11101 ctcctgaata cagtagtatt ttgcagacag aaattccgta aatggattat tcgtttataa
    11161 tcaggataat ctaaaaaaaa ggacccctga aaaagtggtt aacatggaac aaatgaaagt
    11221 tactccttct gtaattgtta tgtcttatgg cctcatccct tttagccgga tactgaaaaa
    11281 catccttcga gagggacggt taccatgaag atttttttgg agaatcttta tcattctgat
    11341 tgctatttcc tgccgataag agataaccag caagaccttg tcggcgttga gttaattacc
    11401 catttctcca gtgaagatgg tacggttcgt atcccgacga gtcgggttat agcgcaactt
    11461 accgaggagc aacattggca attattttct gaacagttgg aattactaaa atcatgccaa
    11521 cactttttta ttcaacataa actctttgcc tggcttaatt taacgccaca agtggctaca
    11581 ttattattag acagggataa tttcgcaggc gaactattaa aatatccgtt tattgaactg
    11641 ttgattaatg agaattaccc gcatttcaat gaaggaaaag ataatcgcga tcttttatcc
    11701 ttatcgcaaa tgtatcccct tgtgttaggt aatttgggag caggtaatag cacaatgaaa
    11761 gcggtgtttg atggtctttt cacccgcgtc atgctggata aaagttttat tcagcaacaa
    11821 atcacgcatc gctccttcga accgtttatt cgtgcgatcc aggcgcaaat ttccccttgt
    11881 tgcaattgca tcatcgcggg gggcattgat acgccagaaa tattggcaca gattataccc
    11941 tttgatttcc atgctctcca gggatgcctg tggcctgcgg ttcctataaa tcagataacg
    12001 acgttggttc agcgataacc ctcctgtttg ctggtgttta ggacgagagt aaccgtctac
    12061 actatcaaac aggaggatct atgaccctgt cttttattac ccgctggcgc gatgaattgc
    12121 cagaaaccta tacagcactt tcccctacgc ctttaaataa tgcccggctg atttggcata
    12181 ataccgaact ggctaacacg ctgagtattc catcgtcgct gtttaaaaat ggcgcaggtg
    12241 tctggggcgg cgaaaaccta ctgcctggta tgtcaccact ggcgcaggtt tacagtggtc
    12301 atcagtttgg cgtctgggcg ggccaactgg gtgatgggcg cggcatttta ctcggcgaac
    12361 aactgcttgc tgatggcact acaatggact ggcatctgaa aggtgctggc ctgacgcctt
    12421 attcgcgaat gggtgatgga cgggcggttt tacgttcgac gatacgagaa agtctcgcca
    12481 gtgaggcgat gcattatctg ggcattccga cgacccgcgc tttaagtatc gtcaccagcg
    12541 attcgccagt gtatcgggaa acggtggagc caggcgcgat gctgatgcgt gtggcaccaa
    12601 gtcatctgcg ctttggtcat ttcgaacatt tttactatcg ccgcgagccg gaaaaggttc
    12661 gtcagttagc tgactttgcc attcgtcatt actggccaca tcttgcagat gatgaggaca
    12721 aataccgtct ctggtttacc gatgttgtcg cacgtaccgc ctcgttaatt gcccaatggc
    12781 agactgtcgg ctttgctcat ggggtgatga ataccgacaa catgtcgctg ctggggctga
    12841 cgcttgatta cgggccgttt ggttttcttg atgattacga acccggtttt atttgtaatc
    12901 actcggatca tcaagggcgt tacagctttg ataatcaacc tgccgtcgcg ttgtggaatt
    12961 tacagcgtct ggcgcagaca ttgtcaccat ttgttgccgt agatggcctg aatgaggccc
    13021 tggacagcta tcagcaggtt ttgttgacgc attatggaca acggatgcgg cagaaactgg
    13081 gcttcatgac ggagctaaaa gaggataacg cgctactgaa tgaattattc agtctgatgg
    13141 cgcgagagcg cagcgattat acccgcacat tccgcatgct gagtctgacc gagcagcaca
    13201 gcgcggcgtc accgctacgt gatgagttta ttgatcgtgc agcatttgat gactggtttg
    13261 cccgttatcg ggggcgtttg caacaagacg agattaccga taatgagcgt cagcaactga
    13321 tgcaaagcgt taacccggct ctggtgttgc gcaactggtt ggcgcaacgg gcgattgaag
    13381 cggcagaaaa gggtgatatg acggaattgc accgcctgca tgaggcgttg cgaaatcctt
    13441 tcagcgacag agatgatgac tatgtcagtc gtccacctga ctggggtaaa cggctggaag
    13501 tcagctgctc gagttaaagg ctacttggtc aacagcagct tgccagcctg agttttacgg
    13561 agcagatatt cttgtccgtc atgatcgata atcagtttgc catccggacc taacagtgtc
    13621 tggctggtaa ttcgccggat gggctgcggg gaagcggtct tgtgattggc atccgtttca
    13681 ggcgtgagtt ttctgctatc cgtataacgc ataaaaatta ataataattt atataacaat
    13741 gattcttatt atcaatatta tggagggcca gagcaagcag taatttgttg agaagacaac
    13801 aagttgcgca gaaatgccca tcctgtcagg aatgggcacg cattcagaag cgggtatcta
    13861 ccgcagaggc gagtttttcg accaggcgtt cggtatcttc ccagcccaga cacgggtcgg
    13921 taatggattg accgtaagtg agcggctgac cgccgacgat tttttgcgtt ccttcgcgca
    13981 ggaaactttc cgccataatt ccggcaatcg ccgtagagcc attgcggatt tgctgacaaa
    14041 tatcctcaca aacttctaac tggcgacggt gctgcttctg gcagttaccg tggctgaaat
    14101 ccaccaccag atgttcaggc aaatcaaatt cgtgcagcgt atcgcaggct gcggcgatat
    14161 catcggcatg ataattcggt tttttgccgc cacgcataat aatgtggcca tacgggttgc
    14221 cgctggtctg atagatggtc atctgaccgt ttttgtctgg cgagaggaac atatggctag
    14281 cgcgggctgc gcggatagca tccacggcaa tccgcgtgtt gccatcggta ccatttttaa
    14341 aacccaccgg acaggagagt gccgaagcca tttcgcggtg gatctgactt tcggtagtac
    14401 gtgcgccaat cgcgccccaa ctgattaaat cagcaataaa ctgaccggtc accatatcga
    14461 ggaactcagt cgcggtaggg acgcccagct catttacctg taaaagtaat ttgcgcgcca
    14521 gctccagacc gtgatttacc cgatagctac cgtttaaatc tggatcggag atgagtcctt
    14581 tccagccgac aaccgttcgt ggtttttcaa aataggtgcg cattacgatt tccagccgtg
    14641 actggtactg gttgcgcagc gactgcagac gggtggcgta ctccatcgca gcggtgagat
    14701 cgtggatcga gcaggggcca ataatgacca acagtcgctt atcttcacca ttcagtattt
    14761 tttcaattct acggcgggag tcggtgacat gggtggcgac gccaggcgtt acgggatacc
    14821 gtagcgcgag ttcggcgggc gttaccaggc tctcaatacg cgcagtacgg agttcgtcag
    14881 ttctgttcat tacaagtctc agaaatttgt tgcttctacg gcaggactac cgggaagtga
    14941 tgcgcatcac gataaaccaa tcgcagctga tttcaagtgc gaggtgtgtc acacctcgcc
    15001 gggtggttag catgataaca aaaaaacaag ctaatgcact agttctctag tacattcggc
    15061 ggctaaggcc catgatatcg aggatcttgg tggcaatctc ttctaccgaa taattggtac
    15121 tgttaatcca cgggatctga tttttacggt acaacgcttc cacttccgcg acttccatcc
    15181 tgcactgacg aagcgaggca tagcgactgt tctcccgacg ttcctcgcga atcgccgcca
    15241 gacgttccgg gtcgatagtc aggccgaaca atttatgctg aagtggtttg agcgacgcgg
    15301 gtagcaccag attatccata tcatcggcaa taaaggggta gtttgccgcg cggataccaa
    15361 attgcattgc cagatacaga ctggtggggg ttttaccaca gcgagaaaca ccgagcagga
    15421 tcacctgagc ctggtccaga ttgcgcaacg aaataccgtc atcgtgggca agggtgtaat
    15481 caatcgccgc aatgcgcgca tcatatttat tgagattatt agggttaagg ccatgggtac
    15541 gatgagcaat cggcgttgga tccagtttca tctcttgttg tagtggggca accagcgcct
    15601 gaacgatatc ctggcaaaag ccttcacttt gcaagatgat agcgcgaatc tccggcaaca
    15661 cgatggagta gaagaccagc gggcgcacac ctgtctggtg ataaattgcg tcaatctgat
    15721 ccttcactgc ccgtgcacgg ctctcatttt cgacaaacgg cagcgtaatg ctgctgatag
    15781 tgacgggaaa ttgtgacatt actgcgtgtc ctaatacctc cgcagttatt gccgtaccat
    15841 cagaaatata aaaaacgtgg cgatcaacag cattatccat tttgttcttc ccgtgatgca
    15901 gacataattc tatgaaagca t
//
LOCUS       DACADF010000110        15778 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.112, whole genome shotgun sequence.
ACCESSION   DACADF010000110 DACADF010000000
VERSION     DACADF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.112"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(3..728)
                     /gene="phoU"
                     /locus_tag="ISB30_003364"
     CDS             complement(3..728)
                     /gene="phoU"
                     /locus_tag="ISB30_003364"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP1990965.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(743..1516)
                     /gene="pstB"
                     /locus_tag="ISB30_003365"
     CDS             complement(743..1516)
                     /gene="pstB"
                     /locus_tag="ISB30_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAP1990966.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1607..2497)
                     /gene="pstA"
                     /locus_tag="ISB30_003366"
     CDS             complement(1607..2497)
                     /gene="pstA"
                     /locus_tag="ISB30_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP1990967.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIASITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2497..3456)
                     /gene="pstC"
                     /locus_tag="ISB30_003367"
     CDS             complement(2497..3456)
                     /gene="pstC"
                     /locus_tag="ISB30_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAP1990968.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3542..4582)
                     /gene="pstS"
                     /locus_tag="ISB30_003368"
     CDS             complement(3542..4582)
                     /gene="pstS"
                     /locus_tag="ISB30_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP1990969.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4896..6725)
                     /gene="glmS"
                     /locus_tag="ISB30_003369"
     CDS             complement(4896..6725)
                     /gene="glmS"
                     /locus_tag="ISB30_003369"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP1990970.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6887..8257)
                     /gene="glmU"
                     /locus_tag="ISB30_003370"
     CDS             complement(6887..8257)
                     /gene="glmU"
                     /locus_tag="ISB30_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP1990971.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPIKKK"
     gene            complement(8611..9030)
                     /gene="atpC"
                     /locus_tag="ISB30_003371"
     CDS             complement(8611..9030)
                     /gene="atpC"
                     /locus_tag="ISB30_003371"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP1990972.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9051..10433)
                     /gene="atpD"
                     /locus_tag="ISB30_003372"
     CDS             complement(9051..10433)
                     /gene="atpD"
                     /locus_tag="ISB30_003372"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP1990973.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10460..11323)
                     /gene="atpG"
                     /locus_tag="ISB30_003373"
     CDS             complement(10460..11323)
                     /gene="atpG"
                     /locus_tag="ISB30_003373"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP1990974.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11374..12915)
                     /gene="atpA"
                     /locus_tag="ISB30_003374"
     CDS             complement(11374..12915)
                     /gene="atpA"
                     /locus_tag="ISB30_003374"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP1990975.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12928..13461)
                     /gene="atpH"
                     /locus_tag="ISB30_003375"
     CDS             complement(12928..13461)
                     /gene="atpH"
                     /locus_tag="ISB30_003375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP1990976.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13476..13946)
                     /gene="atpF"
                     /locus_tag="ISB30_003376"
     CDS             complement(13476..13946)
                     /gene="atpF"
                     /locus_tag="ISB30_003376"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP1990977.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14008..14247)
                     /gene="atpE"
                     /locus_tag="ISB30_003377"
     CDS             complement(14008..14247)
                     /gene="atpE"
                     /locus_tag="ISB30_003377"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAP1990978.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14294..15109)
                     /gene="atpB"
                     /locus_tag="ISB30_003378"
     CDS             complement(14294..15109)
                     /gene="atpB"
                     /locus_tag="ISB30_003378"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP1990979.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15118..15498)
                     /gene="atpI"
                     /locus_tag="ISB30_003379"
     CDS             complement(15118..15498)
                     /gene="atpI"
                     /locus_tag="ISB30_003379"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAP1990980.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         3782 a         4307 c         3950 g         3739 t
ORIGIN      
        1 aattatttat cgctatcttt ttctgccagc agtttatcga gctcgtcacc accgacgtga
       61 cggaaatcct gccccttcac gtagtagaag ataaactcac aaatattctg gcagcggtcg
      121 ccaatacgtt cgatagaacg cgcacagaat aacgcggtaa gtacgctcgg aatggtacgc
      181 gaatcttcca tcatgtaggt catcagttga cgaacaatac cttcgtactc ctgatcgact
      241 tttttatctt cacgataaat acgtaccgct tcgtcaatgt ccatccgcgc gaacgcgtcc
      301 agcacgtcgt gcagcatctg gatggtatga cggcccagcg actccagact taccagcaac
      361 ggctgatgct gctgggagaa tttctccagc gcagtacggc agattttatc cgccacgtca
      421 ccaatacgct ccagctcggc aatggtttta ctgattacca tcaccagacg caggtcgctc
      481 gccgtcggct gacgtttggc gataatgcgc acgcaggctt catcgatcgc cacttccatc
      541 atgttgacgt tcttgtcgcc ttcgatgacg cgcttcgcca gatcgctgtc ctggttatgc
      601 atcgcggtga ttgcatcaga aagctgctgc tccaccatgc cgcccatggt catcacctgc
      661 gtgcggatgc tttccagttc ggcgttgaac tggccggaaa tatgtttatt aagattgaga
      721 ctgtccataa cgcactcctg aatcaaccgt aacgaccggt gatgtagtct tctgtttgtt
      781 ttttcgctgg cttggtgaac agatcgtccg tgttgctgaa ctcaatcaat tcgcccaggt
      841 acataaacgc cgtgtggtcg gaacaacgcg cagcctgctg catgttgtgg gtgacgatca
      901 ccacggtgta atcctgcttc agttcggtga tcagctcttc aatacgcccg gtagagatag
      961 ggtcgagtgc cgaacacggt tcgtcgagca gcagcacttc cgggcgaatg gcgataccac
     1021 gcgcaatgca cagacgctgt tgctgaccac cagagagaga gtaaccgctc tggtgcaatt
     1081 tatctttggt ttcgttccac aatgcggctt tggtcaatgc ccactgcacg cgctcgtcca
     1141 tgtcggcacg ggagagcttc tcaaacagac gcacgccaaa ggcgatgttg tcgtaaatgg
     1201 acatcgggaa cggcgtcggt ttctggaaca ccatgcccac tttcgcacgc agcagggcga
     1261 tatcctgaga gttggtcagg atgttgtcgc catcgagcag aatttcacct tccgcacgct
     1321 gctccgggta cagttcaaac attttgttga aggtacgcag cagcgttgat ttaccgcagc
     1381 cggacggccc gataaacgcc gtaacctggt ttttggcgat atccaggttg atgtttttca
     1441 gggcatggaa tttgccgtag tagaagttca aattacgaac ctgaatttta ctcggggcag
     1501 tttcaaccat actcattgca atcttttcct catcgtgcct gatgcacttc gcgaatcagg
     1561 cttaccgtag gccggataag gcgtttacgc cgcatccagc aaagtgttaa ccgtgtttat
     1621 tcttcgcaaa aacaacgcgc gccagaatgt tcagcagcag tacgcacagg gtaatgatca
     1681 acaccccggc ccaggccagt tgctgccatt ccgcaaacgg gctcatcgca aacttaaaga
     1741 tcgtcaccgg caggttggcg atcggctgca tcatgtccgt gctccagaac tggttggaga
     1801 gcgcagtaaa cagcagcggt gcggtttcac cagcaatacg ggcaatcgcc agcaggatac
     1861 cggtcatgat cccggacacc gacgctttca gcgtgatcga ggcgatcatc ttccacttcg
     1921 gcgtacccag cgcataagcc gcttcacgca ggctgtccgg caccagtttc agcatgttct
     1981 cggtggtgcg gataacaatc ggcacctgca acagcgccag cgcaatcacg cccgcccagc
     2041 cggagaagtg ctccatctgc gccaccacga tggtgtaaac aaaaagacca accacaatcg
     2101 acggcgcgga gagcagaatg tcgttaatga agcgaatcac ttctgccagc caggatttac
     2161 gaccatattc agccaggtaa atccccgcca taatgcccag cggcgtaccg aataccgtag
     2221 cccacaaaat taacaggccg ctacccgcca gggcgttcgc cagaccaccg ccttccgtat
     2281 tgggtggtgg cgtcatttcg gtgaacagcg ccagcgacat accgtcgata ccgcgagtga
     2341 tggtggacat taaaatccag atcagccaga acagaccgaa ggccatcgtc gccattgaga
     2401 gcgtcagcgc aatacggttt ttgaggcggc gacgcgcctg cattttgcgg cgggattcag
     2461 ccagtgccgc agtggtttgc atttcaacca tagccattag cgtgccccct cattcttagc
     2521 caggcgcata atcataaact tcgatgcggc gaggacgatg aaggtaatca caaacaggat
     2581 caggcccagt tccatcagtg cagcaacgtg cagacccgac tctgcttcgg caaattcgtt
     2641 cgccagcgca gaggtgatac tgttgcccgg catgtacagc gaggcgctgt cgagctggta
     2701 ggtgttaccg atgataaagg tcaccgccat ggtttcacca agcgcgcggc ccagccccaa
     2761 cataatgccg ccgataacac catttttggt gaacggaaga acgatacgcc agataacttc
     2821 ccaggtggtg cagccaatac cgtaggccga ctctttcatc atcaccgggg tttgttcgaa
     2881 cacatcgcgc attaccgccg caatgtacgg aataatcatg atggcgagga tcacgcctgc
     2941 cgcgaggata ccgataccaa aggccgggcc ggagaacagc gcgccgacaa tcgggatatt
     3001 cgacatgata ttgccgaccg gctcctggaa gtaaacggcg aacagcggcg caaagataaa
     3061 caggccccac atgccgtaaa cgatacttgg aatggctgcc agcagctcaa tggcgatacc
     3121 cagcgggcgc ttcagccagc caggcgcaag ctcggtcagg aacagggcga taccgaaact
     3181 caccgggacg gcgatcagca gcgcgataaa cgaggtcacc agcgtaccgt agatcggcac
     3241 cagcgccccg tagatatcgt tcggcgcatc ccactctttg gtccacagga aagccagacc
     3301 aaatttctga atgctcggcc aggaggagat gatcagagag acaataatgc cacccaacat
     3361 caatagcaca atcagcgccg ccagttttac cagcacgctg aaaattatgt cgcccttttt
     3421 acccggtggg ttaaaagcag gcttggttgc agccataagt tactcttcag ttaaacgcgt
     3481 ttacaaagtc attttgtaat gccggatgcg gcgtaaaaca ccgtacccgg cctgagtttt
     3541 attagtacag cggcttgccg ctactgtctt taatattggt cttccacgca gcgcgaacct
     3601 gttccactac gctatccggc aggctggcgt aatccaggtc gttcgcctgt ttagccccgg
     3661 ttttgtatgc ccagtcgaag aacttcagca cttctgtgcc ttgttctggt ttcttctgat
     3721 ctttgtggat cagaatgaac gtggtagagg taataggcca cgcatcttcg cctttctggt
     3781 tggtcagatc ctgagcgaat gttttgctcc agtctgcgcc ttttgctgca ttagcgaagt
     3841 tttcttcggt cggactaacc ggtttgccat cagcggagat caatttggtg tacgccaggt
     3901 tgttctgctt cgcgtaagca tattcaacgt aaccaattgc acccggcaga cgctgaacga
     3961 acgcggcgat accgtcgtta cctttaccgc ccagaccgat cggccatttt acggtagagc
     4021 cagtaccaac gttgtttttc cactcttcgt tcactttcgc caggtagctg gtgaagacga
     4081 aggaagtccc ggagccatct gcgcggcgta ctacagcaat gttctgtgaa ggcagtttca
     4141 gacccggatt cagtttggcg atggcttcat catcccactt cttgattttg cccaggtaga
     4201 tgtcgccgag ggttttacca tccagcacca gttcgccaga cttcagacct ggaatattaa
     4261 ccgccagcac cacgccgcca atcacggtcg ggaactggaa caggccttcc tgagccagtt
     4321 tttcatcaga cagcggcgcg tcagaggcac cgaaatcaac ggtgttagcg ataatctgtt
     4381 ttacgccacc ggaagaaccg ataccctggt agttaacttt attaccggtt tctttctggt
     4441 aagtgtcagc ccatttggca tacaccggcg cagggaaggt tgcacctgca cctgtcaggc
     4501 ttgcttctgc aaacacagag aaagcactca tcgataaggt cgcggcgaca acagttgcga
     4561 cggtggtacg cataactttc ataatgtctc ctgggaggat tcataaagca ttgtttgttg
     4621 gctacgagaa gcaaaatagg acaaacaggt gacagttata tgtaaggttt atgacagttt
     4681 tatgacagag agagaaagtc tttagtctga tttaaataag cgttgatatt cagttaatta
     4741 caaatattaa taacgaagag atgacagaaa aattttcatt ctgtgtcaga gaaaaagtag
     4801 ccgaagatga cggtttgtca catggagttg gcaggatatt tgattaaaaa cataacagga
     4861 agaaaaatgc cctgcttacg cagggcatcc atttattact caaccgtaac cgatttcgcc
     4921 aggttacgcg gctggtcaac gtcggtgcct ttgatcagcg caacgtggta agccagtagt
     4981 tgcagcggaa cggtgtagaa gatcggtgca atcacctctt ccacatgcgg catctcaatg
     5041 atgtgcatgt tgtcactgct cacaaaaccc gcatcctgat cggcgaagac atacaactga
     5101 ccgccacgcg cgcgaacttc ttcaatgttg gatttcagtt tttccagcaa ttcgttgttc
     5161 ggtgcaacga cgataaccgg catatcggca tcaatcagcg ccagcggacc gtgtttcagt
     5221 tcgcctgcag cgtaggcttc agcgtgaatg taagagatct ctttcagctt caatgcacct
     5281 tccagcgcga ttgggtactg atcgccacgg cccaggaaca gcgcgtgatg tttgtcagag
     5341 aaatcttctg ccagcgcttc aatgcgtttg tcctgagaca gcatctgctc aatacggctc
     5401 ggcagcgcct gcagaccatg cacgatgtca tgttcaatgg aggcatccag acctttcagg
     5461 cgagacagtt tcgccaccag catcaacagc acagttaact gggtggtgaa tgctttggtg
     5521 gaggccacgc caatttccgt accggcgttg gtcatcagcg ccagatcgga ttcgcgcacc
     5581 agagaagaac ccggaacgtt acagattgcc agcgaaccaa ggtaacccag ctctttcgaa
     5641 agacgcaggc ccgccagggt atccgcggtt tcgccagact gtgacaaggt gatcatcagg
     5701 ctgttacgac gcacggcaga tttgcgatag cggaattcag aggcgatttc gacgtcgcac
     5761 ggaatacctg ccagcgattc aaaccagtag cgggaaacca taccggagtt ataagaagta
     5821 ccacaggcga ggatctgaat atgctcaacc ttcgacagca gttcatcggc gttcggtccc
     5881 agctcgctta aatcaacctg accgtggctg atgcgcccgg taagggtgtt tttgatcgcg
     5941 ttcggctgtt cgtagatctc tttctgcatg tagtggcggt aaataccttt atcgcccgcg
     6001 tcatattgca gattggattc gatatcctga cgttttactt ccgcgccagt tttatcgaag
     6061 atgtttaccg aacggcgagt gatttccgca atatcgccct cttcaaggaa gataaaacga
     6121 cgagtcactg gcaacagcgc cagctggtca gaagcgataa agttttcgcc catacccagg
     6181 ccaatcacca gcggactacc agaacgtgcc gccagcaggg tatccggatg acgggagtcc
     6241 atgatcactg taccgtacgc accacgcagt tgcgggatag cacgcagaac tgcctcacgc
     6301 agagtaccgc cttgtttcag ctcccagttc accaaatgtg caatcacttc ggtgtcggtt
     6361 tcagaaacga aggtatagcc acgcgctttt aactcttcac gcagcggttc atggttttcg
     6421 atgatgccgt tatgcaccac cacaatgtgt tcagaaacat gcggatgcgc gttagcttct
     6481 gaaggttcac cgtgggtcgc ccagcgagtg tgagcaatac cggtgccgcc atgcagagga
     6541 tgttcttccg ctgcctgagc cagcatctgt actttaccga ggcgacgcag gcgggtcata
     6601 tgaccttcag catccaccac ggccaggccg gcagagtcat atccgcgata ttccagacga
     6661 cgtaaacctt caagaaggat ttctgctaca tcacgttgcg cgatcgcgcc aacaattcca
     6721 cacatagttt ttgattccga tttatatcgt tgtcggtcaa cctgtatgcc cgttttttcg
     6781 ggcgccccga gccttgtaga gagtggggtt atttttatag ttactgcttg tgggcgggag
     6841 attatgttat ctcctcatcc catatgaccg gattacccga ccagaattac tttttcttta
     6901 tcggacgacg ccagccttct ttttgagtct gcggcacacg gctgatagcc agggcatttt
     6961 cgccgacatt acgcgtcaca gttgtacccg cagcaatggt cgcgcctttg cctactgtta
     7021 ccggggccac cagttgagtg tcggaaccga caaacacatc gtcgccgata atggtcttaa
     7081 atttattcgc accatcgtag ttgcaggtaa tggttcccgc accgatgtta acgttatcgc
     7141 caatttccgc atcgcccagg taagtcagat gaccagcttt cgagccttta cccagacgcg
     7201 cttttttcat ctcaacgaag ttaccgacgt gtgcaccttc cagcaactca gcaccaggac
     7261 gcagacgggc aaacgggcca atagtacagg ccgctgccag attcgcatct tccacgacgg
     7321 tatatggact gatttcgcaa tcatcgccaa tcacgctgtt tttaatcacg caaccggtgc
     7381 cgattttcac gcgatgaccg agagtcacgt tgccctcgat gataacgtta gtatcaattt
     7441 caacatcgcg cccgtgagta agcgtaccgc gcagatcaaa acgcgccgga tcgcgcagca
     7501 taacgcctgc taacagcagt ttttcagcct gttcggactg gtaaacgcgc tccagtcggg
     7561 agagttgcag gcggttattc acgccttcta cttcgcttaa acgttgcgga tgaacggcga
     7621 cgatttcacg tccttcctga tacgccagcg caataatgtc ggtgatgtag tattcgccct
     7681 gagcattatt gttggtcagc ttcgccagcc agcgtttcat atctgcgccg ttggcaatca
     7741 gaatgccggt gttgatctcc tgaatctgac gctgctcgtc ggtggcatct ttgtgttcaa
     7801 caatgccggt aactttgccg ttttcacggg tgatacgtcc ataaccggtc ggatcatcca
     7861 gttttaccgt cagcagacca atgccaccct gcggtttagc atcacgcaga cgctggagtg
     7921 tttcgacaga gatcagcggc acgtcgccgt agagcattaa aatgtcttca tcatcggcaa
     7981 agaaaggtgc ggcctgctgc atcgcatgac ccgtacccag ctgttctgcc tgaagcaccc
     8041 agttcaggtt gtcgtctttc agcgcctgct taagcagatc gccgccgtga ccatacacaa
     8101 ggtgaacgtg cgctgcgcct aattcattcg cagcatcaat gacatgctga accatcgctt
     8161 tcccggcaag ggtatgcagc actttcggaa gatcggaata catgcgcgtg cctttgcctg
     8221 cggcaaggat cactacgctc atagcattat tcaacatacg cgtcctgact gtaatttgag
     8281 aacgaattta aaccgcttca ccttgaaaaa actacatttt tttcatcgtg aaatggacag
     8341 aggataaatt gttcaataac ggattatccc cgctgacgaa agcgccattt tcgaccatcg
     8401 tcacgccttt tgtctgcaga aaattaaggc agtaccttca tttaagcgtc aggtggatgt
     8461 ttttgctctt atttcgatca atgaataaac gggaaaacag gggtgtttta tatcatcagg
     8521 atctatgtga acgcttttca ggacgggtca atacgcgtaa aaaaaagcca gcctgttgcc
     8581 agactggctt ttgtgctttt caagccggtg ttacatcgct tttttggtca actcgataac
     8641 gcgcagctgc gcgatcgctt tggccagttc cgcagacgcc tgagcgtaat ctacgtcgcc
     8701 gtgagagctg ctaatgtgct cttcagcctt acgtttcgct tccatggctc gcgcttcgtc
     8761 gagatcctgg ccgcgaattg cggtgtcggc cagaacggtc acgttgccag gctgcacttc
     8821 aagaatgccg ccagacagat agataaactc ttcgtgaccg tgctgtttca cgatgcgaat
     8881 cataccaggc ttaatggcgg tgagcagcgg tgcgtggcca gggtagatcc ccagttcacc
     8941 ttcgctaccc gttacctgga ttttctcgac cagaccagag aacatttgtt gctctgcgct
     9001 gacgacgtcc aggtggtaag tcattgccat atcaccctcc gattaaggcg ttaaagtttt
     9061 ttggcttttt ccacagcttc ttcgatggaa ccgaccatgt agaacgcctg ctccggcagg
     9121 tgatcgtatt cgccttccat gatgccttta aagccacgga tggtgtcttt cagggagacg
     9181 tatttacccg gagaaccggt gaatacctct gccacgaaga acggctggga caggaagcgc
     9241 tggatcttac gagcacgcgc taccaccagt ttgtcttctt cagacagttc atccataccc
     9301 aggatggcga taatgtcttt cagttcctga taacgttgca ggatggactg aacgccacgc
     9361 gcggtgtcgt agtgttcctg accaaccacc agcgggtcca gctgacggct ggtggagtcc
     9421 agcgggtcaa cggccgggta gatacccaga gacgcgatct gacggctcag taccacggtt
     9481 gcgtcaaggt gcgcaaaggt ggttgccgga gacgggtcag tcaggtcatc cgcaggtacg
     9541 tataccgcct gtacggaggt gatagaaccg gttttggtgg aggtgatacg ttcctgcaga
     9601 acgcccatct cttccgccag ggtcggctga taacctaccg ctgaaggcat acggcccagc
     9661 agtgcggata cttccgtacc ggccagggtg taacgataga tgttgtcaac gaacagcaga
     9721 acgtcacgac cttcgtcacg gaatttctca gccatggtca gaccggtcag agcaacgcgc
     9781 agacggtttc ccggcggctc gttcatctgg ccatacacca gggatacttt gtcgataacg
     9841 ttggagtcgg tcatttcgtg gtagaagtcg ttaccctcac gagtacgttc acctacgccc
     9901 gcaaacacag agtaaccgga gtgctcgatc gcgatgttac gaataagctc catcatgttt
     9961 acggttttac ctacacccgc accaccgaac agaccaactt taccgccctt agcgaacgga
    10021 cacatcaggt cgataacttt gataccggtt tccagcagtt cctgagagtt tgacagctct
    10081 tcgtaggaag gtgctgcgcg gtgaatcgcc caacgctctt cttcaccgat ctcgcctttc
    10141 atgtcgaccg gttcacccag tacgttcatg atacgaccca gagtcgcttt acctaccggg
    10201 acttcgatcg ggtgttcgag gtcttttaca tccagaccgc gacgcagacc gtcggaggaa
    10261 cccattgcga tggtacgcac gataccgccg ccgagctgct gctgaacttc cagcaccaga
    10321 cgctcattac cattttgcac ctcaagagca tcgtacacgc gcggtacggc atcctgaggg
    10381 aattcgacgt caactacggc gccgattacc tggacaatct ttccagtagc catcttaaat
    10441 cctctacgaa ataacctgtt taaaccgcgg cggcccccga gacgatctcg gtgagttcct
    10501 gagtaatgct ggcctgacga gctttgttgt ataccaactg cagctcttta atcaggctgc
    10561 cgccattgtc ggtcgcggct ttcatcgcca ccatacgggc ggcctgctcg ctggccaggt
    10621 tttcaaccac gccctgataa acctgagatt cgacataacg acgcagcagg gtatccagca
    10681 acgccttcgg atcgggttcg tacaggtaat cccaggattt atgtttcaga tcatcatcat
    10741 ctgatgccgg taacggcagc agctggctga tggtcggaac ctgagacatg gtgttaataa
    10801 atttgttgct gacaatgtaa agcttgtcca gacggccttc gtcgtaggcc tgcaacatca
    10861 cttttaccgg accgatcagt tcggacaagg aagggttatc ccccatgccg gtgacctggg
    10921 caacaacatt gccgcccacg gagttgaaga acgacacgcc tttcgagccg atcattgcga
    10981 ggtcgcattg aacgcctttg tcggtccagg tcttcatttc cgccagcagt tttttgaaca
    11041 ggttaatgtt caaaccaccg cacaaaccac ggtcggtcga caccaccagg tagcccacgc
    11101 gtttaacgtc gcggtcttcc aggtaagggt gcttatattc cagattaccg tgtgcaaggt
    11161 gaccaatcac tttgcgcatg gtttctgcat aaggacggct ggccgccatg cgatcctgcg
    11221 atttacgcat tttggaagcg gcgaccatct ccatcgcttt agtgatcttt tgcgtgttct
    11281 ggacgcttgc gatcttacta cgtatctctt ttgcgccggc catgagcttc tcctcaatgc
    11341 cttgcggcct gcccaaaggc aagccgccag acgttaccag gattgggttg ctttgaagga
    11401 atcgaggatg cctttcagct tgccttcgat ttcgtcgttg tagccaccgg tctggttgat
    11461 ctcttgcatc aacggagcgt gatcacggtc gacgtaagcc aacagagcgg cttcgaagct
    11521 gccgattttc gacagttcaa catccgccag gtaaccacgt tctgctgcga acagaaccag
    11581 agactgttgc gcaacagaca tcggcgcata ctgtttctgc ttcagcagtt cggtcacttt
    11641 ctgaccgtgg tcaagctgct tacgtgttgc atcgtcaagg tcggatgcaa actgagagaa
    11701 cgctgccagt tcacgatact gtgccagagc ggtacggata ccaccggaca gttttttcat
    11761 gatcttggtc tgtgctgcac caccaacacg ggatacggaa atacccgggt taaccgcagg
    11821 acgaataccg gcgttgaaca ggttggtttc caggaagatc tgaccatcgg taatggagat
    11881 tacgttggtc ggaacgaacg cagaaacgtc acccgcctga gtttcgataa tcggcagcgc
    11941 agtcagagaa ccggttttcc ctttcacttc acctttggtg aaagcttcaa cgtattcggc
    12001 gttaacacgc gcagcacgct ccagcagacg agagtggagg tagaatacgt cgcccgggaa
    12061 tgcttcacgt cctggcggac gacggagcag cagggagatc tgacggtaag caacagcctg
    12121 tttagacagg tcatcgtaaa tgatcagcgc atcttcaccg cggtcacgga agtattcacc
    12181 cattgcgcaa ccagcgtacg gtgccaggta ttgcagtgca gcggattcag acgcggttgc
    12241 taccacaacg atggtgttag ccagtgcgcc gtgctcttcc agtttacgta ccacgttaga
    12301 aatggtggac gctttctggc cgatagcgac atagatacat ttgataccgg aatcgcgctg
    12361 gttgatgatg gcatcgatag ccagtgcggt tttaccggtc tgacggtcac cgatgatcaa
    12421 ttcacgctga ccacgaccga ttgggatcat ggagtcaacg gctttataac cggtctgtac
    12481 cggctgatct acggactgac gttcgataac gcccggagcg attgcttcta cagcagagaa
    12541 gccgtcgtga tccagcggac ctttaccatc gattggtgca cccagagtgt taaccacacg
    12601 gcccagcagg ccacggccaa ccggaacttc caggatacgg ccagtacact taactttcat
    12661 gccttcggca aggtcagcgt acggccccat aacaaccgca cctacagagt cgcgctcgag
    12721 gttcagtgcg atagcgtaac ggttacccgg cagggagatc atttcaccct gcatacaatc
    12781 ggccaggccg tgaatgcgga taacaccgtc acttacagaa acaatagtac cttcgttgtg
    12841 agcttcactc acaacattga actgagcaat gcgctgcttg atcagttcgc tgatttcggt
    12901 ggaattcagt tgcatgctcc agtcccctta agactgcaag acgtctgcaa ggcgctcaag
    12961 acgaccgcgt acgctgccat caatgaccat atcacccgct cggatgataa cacctgccat
    13021 tacagactta tcgattttgc aattcagctt aactttgcgt gacagacgtt tttccatcgc
    13081 agcagaaatt ttcgcgagct gttgttcact caacgcggca gcggaaatga cgtctacctc
    13141 agcggtagcc tcactcacag cacgcaggtg aataaactgc tccagaacat ccgggagcgc
    13201 gttaagacga ccattttcgg ccattacccg aatcaggttc tgaccgtttt cgtccagttg
    13261 ctcaccacaa actgcgataa acgactcggc gagcgtttct ggcgcaagcg cgccagagag
    13321 aagctctgcc atttgttcgt ttttggttac ctcggcggca aacgccagca tgtcctgcca
    13381 gcgttctaca ctttggtgtt cgacggcaaa gtcaaaagct gctttggcgt aggggcgagc
    13441 taccgtaata aattcagaca tcagcccctc cctccttaca gttcagcgac aagtttatcc
    13501 acgatgtcgc tgttagcagc ttcatccacg gaacgttcga tgatcttctc ggcgccagca
    13561 acagccagga tagcaacttg cttacgcagc tcttcacggg cacgtttacg ctcggcttca
    13621 atttccgcct gcgcctgggc cacgatttta gtacgttcct gttctgcctc agctttcgct
    13681 tcatccagaa tctgcgagcg gcgtttgtta gcctgctcga tgattacctg ggcttccgct
    13741 ttcgcttttt tcagctggtc ggtcgcgctg gcctttgcaa ggtcaaggtc cttatgtgct
    13801 cgttctgcgg aagcaaggcc gtcagcaatt tctttttgac gtttttcgat ggctgccatt
    13861 aatggcggcc atacgtactt catgcagaac agaacgaaca ggacaaacgc gatggcctgg
    13921 ccgaggattg ttgcgttaag attcacagca caatgcctct atttagttaa cgttctgata
    13981 ttgctcttta aataaaagca acgcttacta cgcgacagcg aacatcacgt acagacccag
    14041 acctacagcg atcatcggga tagcatccac cagacccata acgataaaga actgagtacg
    14101 cagcagagga atcagatcag gttgacgcgc tgcgccttcc aggaatttac ccccgaggat
    14161 gccgataccg atcgcagcac cgattgccgc cagacccatc atcacagcgg cagccatgta
    14221 cagcagatcc atattcaggt tttccatgac agtctccagt ttgtttcagt taaaacgtag
    14281 tagtgttggt aaattaatgt tcttcagacg ccatcgacag atagacgatc gtcagaacca
    14341 tgaagatgaa ggcttgcagc gtaatgatca ggatgtggaa aatggcccac ggcacattca
    14401 ggatccactg tgaccaccac ggcaacagac cagcaatcag aatgaaaatc agctcaccgg
    14461 catacatgtt accgaacagt cgcaaaccga gtgaaactgg tttggacagc aggcttaccc
    14521 cttcaaggat taagttgaca ggaatgaacg cccagtgatt gaacggctgc agcgtcaact
    14581 ctttcgtgaa gccgccgatg cctttcattt tgatgctgta gaacagaatc aggataaata
    14641 cgcccagtgc catagacagc gttacgttca cgtcagcaga cggaaccaca cgcagtgcag
    14701 gcagacccag tacatgttca gcaatgtacg gcagcaggtc gataggcagt aaatccatca
    14761 ggttcatcag gaatacccag acgaagatcg tcagggccag cggagcaatc agcttgcttt
    14821 tgccatggta catgtctttc acgctaccat taacaaagcc gatcaccagc tcaatcgcgg
    14881 tctgaaactt acctggcacg ccgctggttg cctttttggc tacactacgg aataaaacca
    14941 ggaacaacag acccagcacc accgagaaga acatggagtc aatattgatt gtccagaagg
    15001 tggctggggg gttatgtgga tccaccagcg agaatgtacg caggtccagc tgaaggttat
    15061 tcaggtggtg tcctatgtaa tcctgcggcg tcatattttc tgaagccatg atgcctttta
    15121 ccctttgttg ttaattacag ccggtgccag tatctgaacc accagcacca aaacccacgt
    15181 aacgatcagc ggcaagaata ccgcctttaa aaccgccaac gccaccacca gtaacaccaa
    15241 catcgccaga actttgaaag cttcgccaaa tgcgaatgtc caggccaccc ggcctttcgc
    15301 tggtgtatgc gcctggtgac gccaggcaaa tatcataaac aaaacgttag gcagaaagac
    15361 tgccaggccc ccgcttattg cagagacgcc ccagaagggg tctttgaggc tgaacagcaa
    15421 tccacttgct atcaccacca gtaactgaac gagcagaagc ttccgagcaa cgtttcgact
    15481 cacgagcgac acagacatca cgtttttcac tcctgctccc ttcgaggtat gccgcgtgtc
    15541 gtataaaact ttctttaagg cttagagtca agcatcaaaa agcggtcaaa ttatacggtg
    15601 cgcccccgtg atttcaaaca ataagtagcc aaaaggtgaa taaatgttta aatatttttc
    15661 cagtgcatac aattgcgact tttctgcaaa ccctgttcga tcatgaaaaa ctgtaaataa
    15721 cgcgtaaaca ctggtgataa agcgtgcttc agatcacata ttgcgcatgt tcgcgcac
//
LOCUS       DACADF010000111        15571 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.113, whole genome shotgun sequence.
ACCESSION   DACADF010000111 DACADF010000000
VERSION     DACADF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.113"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            51..617
                     /locus_tag="ISB30_003380"
     CDS             51..617
                     /locus_tag="ISB30_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAP1990981.1"
                     /translation="MSFTNTPERYGVISAALHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLSDAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            621..1196
                     /locus_tag="ISB30_003381"
     CDS             621..1196
                     /locus_tag="ISB30_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAP1990982.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGNLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1238..2290)
                     /locus_tag="ISB30_003382"
     CDS             complement(1238..2290)
                     /locus_tag="ISB30_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP1990983.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2515..3435
                     /locus_tag="ISB30_003383"
     CDS             2515..3435
                     /locus_tag="ISB30_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAP1990984.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3607..4833
                     /gene="mdtG"
                     /locus_tag="ISB30_003384"
     CDS             3607..4833
                     /gene="mdtG"
                     /locus_tag="ISB30_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HAP1990985.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQISN"
     gene            4916..5290
                     /locus_tag="ISB30_003385"
     CDS             4916..5290
                     /locus_tag="ISB30_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HAP1990986.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(5291..5518)
                     /locus_tag="ISB30_003386"
     CDS             complement(5291..5518)
                     /locus_tag="ISB30_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAP1990987.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5690..8233)
                     /gene="mdoH"
                     /locus_tag="ISB30_003387"
     CDS             complement(5690..8233)
                     /gene="mdoH"
                     /locus_tag="ISB30_003387"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAP1990988.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8226..9761)
                     /gene="mdoG"
                     /locus_tag="ISB30_003388"
     CDS             complement(8226..9761)
                     /gene="mdoG"
                     /locus_tag="ISB30_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAP1990989.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10155..11312
                     /gene="mdoC"
                     /locus_tag="ISB30_003389"
     CDS             10155..11312
                     /gene="mdoC"
                     /locus_tag="ISB30_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAP1990990.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNLRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11320..12801)
                     /locus_tag="ISB30_003390"
     CDS             complement(11320..12801)
                     /locus_tag="ISB30_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAP1990991.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADHIELPASWHNDVMTHRYLRKMESSPFIHHLVDGTLPLIWAKTCLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(12743..13276)
                     /gene="ymdB"
                     /locus_tag="ISB30_003391"
     CDS             complement(12743..13276)
                     /gene="ymdB"
                     /locus_tag="ISB30_003391"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAP1990992.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13371..13682)
                     /locus_tag="ISB30_003392"
     CDS             complement(13371..13682)
                     /locus_tag="ISB30_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP1990993.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPRKTYSSKALMSGNVKNVQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13803..14135)
                     /gene="csgC"
                     /locus_tag="ISB30_003393"
     CDS             complement(13803..14135)
                     /gene="csgC"
                     /locus_tag="ISB30_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAP1990994.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14195..14653)
                     /gene="csgA"
                     /locus_tag="ISB30_003394"
     CDS             complement(14195..14653)
                     /gene="csgA"
                     /locus_tag="ISB30_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAP1990995.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14694..15149)
                     /gene="csgB"
                     /locus_tag="ISB30_003395"
     CDS             complement(14694..15149)
                     /gene="csgB"
                     /locus_tag="ISB30_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAP1990996.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3891 a         3984 c         3678 g         4018 t
ORIGIN      
        1 tctccgacac gccattttct ttctctcgca aattcgtttt ggatgtactt atgtcattca
       61 caaatacccc tgaacgctat ggtgttatat ctgcggcctt acattggcta agcgcaatta
      121 ttgtctacgg catgtttgct ttaggtctgt ggatggtcac gctcagttat tacgatggct
      181 ggtatcacaa agcacccgaa ctgcataaaa gcatcggtat tttgttaatg atggggctgg
      241 ttattcgcgt tctgtggcgt gtcatatctc caccgccggg tccgctgccg agttattcgc
      301 ccatgactcg tcttgcagca aaagctggac atctcgcatt gtacctattg ctgttcgcca
      361 tcggcatcag cggctatctg atctcaactg ccgatggtaa accgatcagc gtttttggct
      421 ggtttgacgt ccccgcgacc ctctccgacg ctggcgcaca ggctgacttt gccggtgccc
      481 tgcatttttg gctcgcgtgg agcgttgtag tactgtccgt tatgcacgga tttatggccc
      541 tgaagcatca tttcatcgat aaagacgaca ctctgaagcg catgctggga aagtcgtcat
      601 ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg tttaaccttc gcttccttga
      661 tgttctctgc cggttcagcg gttgccgccg attacaaaat tgacaaagaa ggtcagcacg
      721 cctttgttaa cttccgaatc cagcatcttg gctatagctg gttatatggc accttcaaag
      781 atttcgacgg tacttttacc tttgatgaaa aaaatccagc tgccgataaa gtgaatgtga
      841 caattaacac caccagcgtc gatactaatc acgccgaacg cgataaacat cttcgcagtg
      901 cggatttcct caataccgca aaatatccac aggcaacatt cacctccacc agcgtgaaga
      961 aagacggtga cgaactggat attaccggca atctgacgct gaatggtgta accaagcccg
     1021 tcacactgga agcgaaatta attggtcagg gtgacgaccc gtggggcggt aaacgtgcag
     1081 gctttgaggc cgaaggcaaa attaagctca aagacttcaa tatcaagaca gatttaggtc
     1141 cagcgtctca ggaagtggat ctgattattt cagtggaagg cgtacagcag aagtaagagt
     1201 gaaatgttgc cggatggcac gcatccggca atgatattta ttctgttgga tcaggaatgc
     1261 ccagtgttgt attcagacgt ccacgagact tattaaagat cttattgcca ttttcacgtc
     1321 ctgcccgacg gcgtcgctgt tcctctggcg gtaacgcgct ttcttcgcag caaatctcac
     1381 tacaacaacc tttgtatttt tccgcacata ctggacactg aataaacagc agatggcagc
     1441 catcattttt acagttggta tggctgtcgc acggcgcacc gcactggtgg cagtgcgcga
     1501 taatctcatc agatatacgt tcgcccatcc gctcgtcaaa aacaaaattt ttgccaataa
     1561 aacgcaccgg caagccctgc tcgcgcgcct tacgggcgta ttcaataatt ccgccctcga
     1621 tatgccagac tttgttgaat ccgttatgtt tcatccaggc actggccttt tcacaacgaa
     1681 tgccgccggt gcagtacatg acgatttttt tatctttatg tgcctgcatc atctcaactg
     1741 cttttggcag ctgctcacgg aaggtatctg ccggtatttc cagcgcgttt tcaaagtgcc
     1801 ccacttcata ctcatagtgg ttacgcatgt cgataaacaa tgcatcggga tcgtcaagca
     1861 tggcgttcac ttccgccgct tgcagatact caccaacatt gctggcatca aagtgaggat
     1921 cgtcaatacc gtcggcaaca atgcgatcgc gtaccttcat acgcagtacc cagaaggatt
     1981 tcccgtcatc atccaacgcg atattcaggc gtaaaccctc taaagccgga tcgaaggcat
     2041 agagctgtgc gcgaaatgtt tcaacattgc tcgcaggtac gctgatttgc gcgttaatgc
     2101 cctcatgcgc cagatacact cgcccaaaaa cattcagcgc ggtaaacagt tgatataaag
     2161 cgtcacgggt caccttagga tcggcaatgt ggaaatactt ataaaacgaa atggtggttc
     2221 gcggttcgct ctcagccaac attttggctt ttaacgcgtc gttggaaatg cggttgtgta
     2281 acactggcat ggtgtacggt tcctgcgaga tgggaaagta aaaatccgcg gcatgatata
     2341 gcaattatcg ataattaaca tccacacatt ttacgctaca ttttagcatt aaaaattatt
     2401 tgttatttac aagcgcggca atttcgccca gtcttcagtc acaaattttg gttgcggacg
     2461 aaaaaatgcg acaatacata caattgcccg tataggttga aaaacaggat tgatatgacg
     2521 aatctaccca agttctccac cgcactgctt catccgcgtt attggttaac ctggttgggt
     2581 attggcgtac tttggttggt cgtgcaattg ccctacccgg ttatctaccg cctcggttgt
     2641 ggattaggaa aactggcgtt acgttttatg aaacgacgcg caaaaattgt gcatcgcaac
     2701 ctggaactgt gcttcccgga aatgagcgaa caagaacgcc gtaaaatggt ggtgaagaat
     2761 ttcgaatccg ttggcatggg cctgatggaa accggcatgg cgtggttctg gccggaccgc
     2821 cgaatcgccc gctggacgga agtgatcggc atggaacaca ttcgtgacgt gcaggcgcaa
     2881 aaacgcggca tcctgttagt tggcatccat tttctgacac tggagctggg tgcgcggcag
     2941 tttggtatgc aggaaccggg gattggcgtt taccgcccga acgataatcc actgattgac
     3001 tggctacaaa cctggggccg tttgcgctca aataaatcga tgctcgaccg caaagattta
     3061 aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct ggtacgcacc ggatcatgat
     3121 tacggcccgc gctcaagcgt tttcgtcccg ttgtttgccg ttgagcaggc tgcgaccacg
     3181 accggaacct ggatgctggc acggatgtcc ggcgcatgtc tggtgccctt cgttccacgc
     3241 cgtaagccag atggcaaagg gtatcagttg attatactgc cgccagagtg ttctccgccg
     3301 ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag tggtcgaaaa atgcatcatg
     3361 atggcaccag agcagtatat gtggttacac cgtcgcttta aaacacgccc ggaaggcgtt
     3421 ccttcacgct attaaatctt ccatgctgga tgcttcagaa tggcatccgg cattaccaca
     3481 gaaaatcccc ctgatttagc gataaaagct ctctggattg cgccccctgg aagtcgggcg
     3541 cataattagt gtgcttatct tttcttctta tgttcaccgc gcctggcgca ccaacagcgg
     3601 attgctatgt caccctgtga aaatgacacc cctataaact ggaaacgaaa cctgatcgtc
     3661 gcctggctag gctgttttct taccggtgcc gccttcagcc tggtaatgcc cttcttaccc
     3721 ctctacgttg agcagcttgg cgttaccggc cactccgccc tgaatatgtg gtccggtatt
     3781 gtcttcagca ttacattttt attttcggcc atcgcctcac cgttttgggg tggactcgcc
     3841 gatcgtaaag gccgaaaact catgctatta cgctctgctc tcggcatggg catcgtgatg
     3901 gtgttgatgg gactggcaca aaatatctgg cagtttttga tcctgcgggc gcttcttggt
     3961 ttacttggcg gatttgtccc caacgctaat gctcttatcg ccacacaagt accgcgtaat
     4021 aaaagcggct gggcgctggg tacgctctcc acaggcggcg ttagcggcgc gttgctcggc
     4081 ccaatggctg gcggtttgct cgccgatagc tacggcttac gtccggtatt ctttattacc
     4141 gccagtgtgc tcatactctg ctttttcgtc accctgtttt gcatcagaga aaaattccag
     4201 ccggtcagca aaaaagagat gctgcacatg cgggaagtgg tgacatcact taaaaacccg
     4261 aaactggtac tcagcctgtt tgtcaccacg ttaatcatcc aggtggcgac gggctcaatt
     4321 gcccccattc tgacgctgta tgtccgcgaa ctggcgggta acgtcagtaa cgtcgccttt
     4381 atcagtggca tgatcgcctc ggtgccaggc gtggcggctc tgctaagtgc accacgactc
     4441 ggcaaacttg gcgatcgaat cggacccgaa aagatcctga ttacggcgct gatcttttct
     4501 gtactgctgt tgatcccaat gtcttacgtt cagacgccat tgcaacttgg gattttacgt
     4561 tttttgctcg gtgccgccga tggtgcacta ctccccgccg tacagacact attagtttat
     4621 aactcgagca accagatcgc cgggcgtatc ttcagctata accaatcgtt tcgtgatatt
     4681 ggcaacgtta ccggaccatt gatgggagca gcgatttcag cgaactacgg ttttagggcg
     4741 gtatttctcg tcaccgctgg cgtagtgtta ttcaacgcag tctattcatg gaacagtcta
     4801 cgtcgtcgtc gaatccccca gatatcgaac tgatttttcg cctttcatac ttgcaaaagc
     4861 ggagaatcag ctatcctttt ccctgaaacc tcatcaactc aaagggagaa tcgtgatgac
     4921 catgtacgca acgcttgaag aagccattga cgctgcacgc gaagaatttc ttgcagacaa
     4981 ccccggcatc gacgccgaaa acgcgaatgt gcaacagttc aatgcccaaa aatacgtttt
     5041 gcaggacggc gacatcatgt ggcaagttga gttttttgcc gacgaagggg aagaaggtga
     5101 atgtttaccc atgcttagcg gtgaagctgc gcaaagtgtc tttgacggcg actatgatga
     5161 gatagagatc cgccaggagt ggcaagatga gaatacatta catgaatggg acgaggggga
     5221 atttcagctt gagccaccgc tggataccga ggaaggacgc acagcagctg atgagtggga
     5281 tgaacgttaa tcactcatac gggccatgat gaatgtcgat cggcagcagt aaggtatcga
     5341 aaaccaggga gaatggcaga tcaaggatcg tgacgtaacg ccaggcggag tcacgcacgt
     5401 cccattgcac accgggataa tattggttgc catgcccctg ccccggtata gtgcgactaa
     5461 tgatgctgcc acagccactc agcaaggtca ccattatgct caccacaatt aaacgcattt
     5521 gatacctctt ccgtaaaaaa taccggcata acgccggtat ttttattggc tcgttgtttt
     5581 tgccggatgc ggcgtgaacg ccttgccaga cccacaaaat cttgcaaaat caataaattg
     5641 caggaacgat gtaggcctga taagcgtagc gcatcaggca actacgtttt tattgcgaag
     5701 ccgcatccgg tttacgcaat gccagtggat tgagctttat cccttcgtaa taactcaccc
     5761 atgaagaata tctctccggg gaattccaga cgcggaaatg cagacgggcc atcgtcaccg
     5821 gatcgcttag cagcaccagg cgacgatcgc gattcagctt ctctggcgtc tcgttcagcg
     5881 cctgttcaac gtggcggtca cgggcgattt ccagcacctt gctggcgcgg tgacgcgcgg
     5941 tcgccattgc ggttgccaga gcgttaaatg acgggttaaa caccgcgtgc ataaaaccat
     6001 catcaaggga gcgttgacga ttcatctcaa ggaaccgatc ggtatcaacc agcacctgcg
     6061 gcggtgaata ctcttccggg atcaggaaca gtttccagcg tttggttcgc agaccaacgg
     6121 tggcacggct ggaaatcacc gaaacaaacg gtgacaggat caacgagaag acaatcggtg
     6181 ccagccagaa cagaaaacgc agatccagcc acgccatccc aacagcccac actaacccta
     6241 acagcagctg tgagccgtgg cgtttgaacg cttcccccca ggaagtggag tcatcatcac
     6301 gctgcggcga attccacact acttcccagc caaggaacgc gctgacgacg aagaccgtat
     6361 ggaacagcat gcgtaccgga gccagcagca cggaaaacag cacttccagc agcaacgata
     6421 atgtaacgcg ccagaagccg ccgtattctt tcgttccttt gcaccagata agcaaaatgc
     6481 tcaatagctt cggcaggaac aacagcacca tggtcgaagc aaaaagtgca atcgccagct
     6541 caggacgcca ctgcggccat accgggaaca actgccgtgg ttgcaggaag tattgcggtt
     6601 cggtcaacgc atgtactacc tgcaatgcag tagagagcgc gaggaacata aaccacagcg
     6661 gagcggagag ataagacatc acgcccgtca ggaacaccgc acggtgaacc gggtgcatac
     6721 ccttcaccag gaacagacgg aagttcatca ggttaccgtg gcaccagcgg cggtcacgtt
     6781 ttagctcatc aagcaagtta ggcggcaatt cttcataaga acccgggaga tcgtaagcaa
     6841 tccagacccc ccaacctgca cggcgcatca acgccgcttc cacgaagtca tgtgacagga
     6901 ttgaaccggc aaaagaacct tcgcccggca gcggagccag tgcacagtgc tcgataaacg
     6961 gtttcacgcg gataatcgcg ttatgccccc agtagtgcga ctcgccaagt tgccagaagt
     7021 gcaaaccggc tgtaaacagt ggcccataca cgcgggtcgc gaactgctga cagcgcgcat
     7081 acagcgtatc catgccggac gctttcggcg acgactgaat gatcccggcg ttcgggttgg
     7141 cttccatcag gcgcaccagg ccgcacaaac aatcaccggt cattaccgag tcagcatcca
     7201 gcaccaccat gtagctgtac tggctgcccc agcgacggca gaagtcatcg atattaccgc
     7261 ttttacgctt cacgcgacgg cggcggcggc gatagaaaat ctgaccttcg ccaccgactt
     7321 cagcgataag ctccatccag gctttttgct ctgcgacgca gatatccggg ttataactgt
     7381 cactcagaat gtagacatca aagtgtttgg cattcccggt ggcttttact gattcccacg
     7441 ttgcacgcaa gccagcaaaa acacggttca cgtcttcgtt acagataggc atgatcaacg
     7501 ccgtgcgatg ctccgggttt aatggttcat cgccaactgt tgacgcagat atactgtatt
     7561 tatcgcgacc aataagcagt tgcaggaagc ccatcaacgc cgtccagaac ccggcggaca
     7621 cccaacagaa cagtaccgca aagaggatca ggataccggt ttgcagcata taaggcagaa
     7681 gctgcataaa ggaaacccac acatcctgac caaccatatc cataggatta atcagcgccc
     7741 acccctgata aggaagaatg gtcttcatat accaggtcgc gacaacagtt tgcgcgagcg
     7801 tcaggatcaa cagaatgtaa cggcggatgg taccgacggt acgccacttt tgctcactct
     7861 cctgctcttc tttggtcaaa cgagccagat agcgcggcgt cacatcgcgt ccacgcaggc
     7921 gatcccagaa acggcctacc gggttggtac gccacgggtc gggaaacatc gaggagcgtt
     7981 ttgcttctgg catcgccttt agctgatcgc gcccttcgtc gtctttaatt aactgtccat
     8041 cagcaagtga atctggccag gcttgttcca gacgcgcctt taccgagcct tgcggggagt
     8101 catcctcccg cgcccaggtg cggtgttcgg catccagcgc ctgatgaacg gcgcggatat
     8161 cagtcttcgg caatgccgct ttctcgcttg cggcgatggg cattgcgtca atgtactcag
     8221 ttgtcttatt cattggcagg taactggtag ctccaggttt cactcaacgt ctgatcggca
     8281 ttcaccagcg cagcacgcat ttcagtggtt ttcttggcat ctttcacttt cacacgcatc
     8341 accagacgcc aacctttggt aaccgggtta tagcgcaccg tgctttcaac tatctcacca
     8401 ttatcaccaa tgctggtttg cgctgtgacc ggggtatcct ctggcagttt tttcatctca
     8461 gcgccggtaa aatcgaccac aaaggcgata gtaccgtcag gctggcgaat caggttcgac
     8521 tgcttcacat cccccgttga acgacgcgtt tgttgcaccc atgcgttatc tggcgcatgc
     8581 agtttgtctt catcacggct gaaggtgatg gtgtatttaa agttcatctc tttacccggc
     8641 tccggcagct gatccggcgt ccagtaagcg acgatgttat cgttggtttc atcgttagtt
     8701 ggaatttcca ccagctcaac gctgcctttg ccccactccc ctttcggagt cacccatgcg
     8761 cttggacgaa gatcgtaacg atcatcgaga tcttcaaagc gggagaaatc acgaccgcgc
     8821 tgcaacagac cgaagccttg cgggttttcc atcgagaagc tgctgacagc taaatgtttc
     8881 gggttattca acggacgcca gatccattcg ccattaccag catggataga cagaccgtta
     8941 gagtcgtgca actccggacg atagttattt gcaggcgacg gttggttcgg cccaaacagg
     9001 aacatactgg ttaacggtgc aacccccagt ttgccgactt tatcgcgcag atagattttc
     9061 gactgcacat ccacaaccgt gtcacgtcct ggcataacta cgaatttgta agcacctgtc
     9121 gcgcgcggcg agtcaagcaa tgcataaatg gttaaacgtt tatcagtcgg ttttggacgc
     9181 tcgatccaga actctttgaa gcgtggaaat tcttcacccg atggcaaggc ggtatcaatt
     9241 gccaggccgc gtgcagaaag gccataaacc tgacctgcac caatcacgcg gaaatagctg
     9301 gccccgagca tgctgacgat ttcatcgttt ttatctttgc tgttgatcgg gtaaagcact
     9361 ttgaaaccgg caaaaccaag gtcttttacc gtgtctttgt catgctgaac atcgccgaaa
     9421 gtgaaataat ccgggctgta tttgattcgt ttgactgcgg tggcagtcac ttcatttatt
     9481 ttgaccgggg tatcgaagta cataccctga tggtagaact cgagtttgaa tggggtcttc
     9541 agattgttcc agtacgcttt gtcatgatta aactggatct gctgatagtc cgcgtatttc
     9601 atatcgcgga aaacggaggg caagttgctt ttgggcgcct catagccttt cccggctaag
     9661 gattgagctt gctttgcgac atcatcaata ctgaaagccc agcttgaaga tgtatacagg
     9721 gttaacatta ctgcagcact caaccaacgc attttcatca tttgtagttt atgtttcata
     9781 attagtaagc acttccccct ttgtgtgctt atatcgatcc gatctatttt aatggataac
     9841 ctgccattcc gacaacagaa tcccggtatc gttcagcaca ctggctcatg gattaagcac
     9901 ctgattgcaa ccgttttcac tttccgtgct tctcctaaag acagaagcat cgacttcgtg
     9961 tagggtttgc cagcaaattt atttatcaac cgtcttacag acgcttccat tctggttagt
    10021 ggtagcgatg atgctatgca taaaaacctt gaagcctgcg cctggcgcgg tcaggtcggt
    10081 gtcactatgg caacaagcct aaaggcataa cccgacaggg ataagacgaa aaatctgaga
    10141 tcagttaacc atatatgaat ccagtacccg cgcaacgtga atatttcctc gactccatcc
    10201 gcgcctggct gatgttgtta gggatccctt ttcatatttc tttaatctat tcgagccata
    10261 catggcatgt gaatagcgcc gagccgtcat tgtggctgac cctttttaat gacttcatcc
    10321 actcgttccg catgcaggtg tttttcgtta tatccgggta cttttcctac atgctttttt
    10381 tacgctatcc cttgaaaaaa tggtggaaag tacgtgtcga acgtgtaggt atcccgatgt
    10441 taacagccat ccccttactg acattgccgc aatttattat gctgcaatac gtcaaaggca
    10501 aagcggaaag ttggcctggg ctgtcattgt atgacaaata taatacgttg gcctgggaat
    10561 taatatcaca cctgtggttt ttactggtgt tagtggtcat gacgacgctg tgcgtatgga
    10621 tatttaaacg catcagaaat aatttagaaa attctgataa aacgaataaa aaattctcga
    10681 tggtaaaatt atcggtgatt tttttgtgcc tcggcatcgg ttatgcggta ataagaagaa
    10741 cgatttttat tgtgtatcca cccattctga gtaacggcat gttcaatttt attgtcatgc
    10801 aaacgttatt ttatttgccg ttctttatcc tcggcgcact ggctttcatt ttccctcatc
    10861 ttaaagcctt gtttaccacg ccttctcgtg gctgtaccct tgcagcagca ttggcgtttg
    10921 tcgcttattt actcaacctg cgctatggca gtggcgatgc ctggatgtat gaaaccgagt
    10981 cggtgatcac catggtcctc ggtctgtgga tggtgaatgt ggtcttctcc tttggccacc
    11041 gtttgcttaa cttccagtca gcgcgggtga cttattttgt taacgcatcg ctgtttatct
    11101 atctggttca ccacccgtta acgctgtttt tcggcgcata cattacacca cacatcacct
    11161 ccaactggct tggttttctc tgtggcctga tattcgtagt agggattgcg ataattctgt
    11221 atgaaattca tttacgcatc ccattattga agtttttgtt ctctggtaaa ccggttgtta
    11281 agcgtgagaa cgataaagca cctgcccgtt aagccgcgtt tacaataacc attccacggg
    11341 caatatcgac gccagtttga ccataacccg cttccagaaa ctggtggcgg gttctttttt
    11401 gagaataatc tctttctttg catgacgatc aacccagttg atccgtcccc acctgtccag
    11461 acggagctgc caggccgcat catactggct ctgaataaag cgtttatcaa ttaactgtgc
    11521 cagcgtttcg ctctctatca cgaagcccat ttcagtattg agcaatgttg aacgcggatc
    11581 gaaattgaaa gagccaataa acaccgtttt accatcgatg ctaaaggttt tagcatgcag
    11641 gctggcaccg gaattaccgg ttataccgcg atcgtgtaat gtactacttt gttcacgcgt
    11701 cggcttgagt tcatataatt ccaccccata gcgaagcaat tttttgcgcc agcgcgcgta
    11761 tccggcatgg acgacagcaa catcgttagc ggcaagagaa ttggttagga tcgcaatctt
    11821 tacccctttt ctcaccatcc gtaagagttg cgccacacct gcacgtgtcg gtacaaaata
    11881 ggaagagata atatcgatgc gttcactggg tgagcccatg atatcgaaca ggcgctgcgg
    11941 taacagtgaa tgccgttttg ccttgccctc ccctttcgcc ggatcatcac ttaataaaca
    12001 tgttttcgcc cagataagcg gcaacgttcc atcaaccaga tgatgtataa acggactgga
    12061 ttccattttg cgtaagtaac gatgcgtcat gacatcgtta tgccaggagg cgggtaactc
    12121 gatgtgatcc gccatttcac cttccgggac atccagcacc tgctgtaagg gggaaaccga
    12181 tttgcaatac cagtagcggg cgaaatcatc ggcaacgtcc tctaccacgg gtcctattgc
    12241 catgacatct aaatccgaaa aaagtggctc ttcccctgcc ccaaaatagg catcaccaat
    12301 atttcgtcct cccaccagcg tcaccacgcc atcgacagtg aaacttttat tgtgcatacg
    12361 gcgattgaga cgggaaaagt cggtgatata accaagcgga cgtagcaaac gaaacgagaa
    12421 aggattaaaa agccggactt caatgcgtgg atggctgtca agcaagcgta aaatgtcatc
    12481 aagtccgggc gtattgttgt cgtccagcaa caaacggacg cgaacgccac gctttgctgc
    12541 ggctaacagg gcggaaaaca gtaaccgacc cgacatatcg tcctgccaga tgtaatactg
    12601 aacatccagc gtatgctctg ccatttcagc cagacgatac cgggcggcaa acgcatccag
    12661 acttttctcc agaggaaaaa gtccacactg accgggatgt tgcgaacaca gtggcagcac
    12721 cgcgctcgcc agccgaggca aatcattcat ctccttgttg ggtaaggagt ctttcgtaga
    12781 ggtgggcgtt ttcttcatca taacagacaa agtatacctg ttcgggtaaa gcgtgacggg
    12841 taataaattc tgaaacggtt tttactgcga tttcagccgc tgccgcacgg ggataaccat
    12901 aaaccccagt actgattgca ggaaaagcca ctgacgtata gctgtttgcc gccaccagtc
    12961 gtaggctatt gagataggca tcctgcaaaa gctggtcttc gttttgttca ccaccacgcc
    13021 agactggccc gacggtgtgc actacggctt tagcgggaag atcgcctgca agcgtaataa
    13081 cggcatgccc cgtagggcaa tcgccctgct gctgcctgac ttttaaacag gcatccagca
    13141 gggccggacc cgctgcgcga tgaatggccc catcgacgcc gccgcctccc attaatgacg
    13201 gattagccgc attcacaatc acatcaacgg ccagtttggt aatatcaccc tgcacaacat
    13261 gaatacgcgt tttcataacg cctccttaca ccttcttgtt tcctaagtgt acagcaggct
    13321 ttttagttaa atcgcatttt tctgcttagc aacgtgagtt gttcagaaac ttagttatat
    13381 tcgatgttca ttacagccag ctttttctgt ttatcaagat actgcacttt tactgaggct
    13441 atttgcacgt ttttaacatt gccgctcatt aaggctttgc tggaataggt tttacgcgga
    13501 acagaaccat tctgtgggca gttcatttct acgggtgatg actgggtgct gacgtcacat
    13561 gccggttcca caatttggcc ataaaaatgg atcacgcccc cttgcgcggt gcttcctgca
    13621 atggcaataa aaggaaaaaa caaactgccg agcataagag agtttaaaaa aggtctgaac
    13681 atttattcta tttcctcaat gattagtcat ccttgagggt tgtgttatcc atactttcag
    13741 gttggctgat tcgcagcaga ccattctctc caggttcatc ttatgctcga tatttcaaca
    13801 aattaagact tttctgaaga gggcggccat tgttgtgata aatgaagtga ctgtccatca
    13861 gaaacagtaa caactatttt cacccgatcg tccggggaaa tatttaaact caacttcgtc
    13921 aaagcaatgg gttgattagc aggcaatgag agggtctttt cttgcttcgt ctgactttgc
    13981 cctgaactgc cttcgcgcag ggacaatatt tgtactctgc acagacaaga ttgagtaaga
    14041 gtgacttcag gaataatggt gtacatatcc ccttgctggg tcgtattaaa ggttatctga
    14101 ctggaaagtg ccgcaaggag taataacgca ttcatattct tctcccgaaa aaaaaacagg
    14161 gcttacgccc tgtttctttc atacagatga tgtattagta ctgatgagcg gtcgcgttgt
    14221 taccaaagcc aacctgagtg acgttaacgc tggagttaga tgcagtctgg tcaacagcag
    14281 caccgttacc gccaccgaac tgtttaacag tcatagtaga atctttacca ttccactgat
    14341 caagagtagc gctgttgccg aaaccacgct gagtcagatc gattgagctg tcatcagaac
    14401 cttggccaac atctgcacca ttaccgccgc cgtgctgggt aatggtcaga tcagagttgc
    14461 gggcgtcagc ttgcagagca agagcagagt taccgccacc gtactggtaa atattcagct
    14521 ctgaattcgg gccgctgtta ttaccgccac caccgtggtt gccgccaccg ccgccgtact
    14581 gaggaacaac acctgccaga gcgctaccgg agaatacgat tgctgcaatt gctgctactt
    14641 ttaaaagttt catgtaaaac ccccatcgga ttgatttaaa agtcgaatgg aaattaacgt
    14701 tgtgtcacgc gaatagccat ttgcgactgt ctctgcacta caattgccgt tttttgagta
    14761 ccatactgtg taatatttgc tttattacca gaacctttct ggataatcat cgcagtatta
    14821 ccataagcac cttgcgaaat actggcatcg ttggcactgc ccgcctgatc aatatatgca
    14881 aggttataat ctcctgtctg gtcaatcttt gcccggttgc tactaccttc ttgcgcaaca
    14941 accgccaaaa gttttgagcc tccctgccgt aactgagcac tattattagt cccagcttga
    15001 ccaattatgg ctgcctgatt aaatgaagac ttactcaatt catttaccgc aaagttatat
    15061 tctgaattag ctaaatcata acctgctgcg gctgcaatcc caggcgcacc cagtattgtt
    15121 aacatcataa ataacaattt gtttttcatg ttgtcaccct ggacctggtc gtacatttaa
    15181 gaaattaaat catttcaact tggttgttaa cgcaacccgt attttgttaa cgctgcgtta
    15241 cgatggaaag tatgtctgcg gaaatatttt taataactca cccgcgcgtt gtatttttct
    15301 ttttttgttc ataccaaagt attaaaaccc acgtaaatac gctgttatct acgcaaaaaa
    15361 tattttgttt tcttttaaat ctccgttttc cgctatcaaa aatcaccaga cagtcattct
    15421 tcttgcccgt cgctgattgc tgcgatatgt cttgcgcaca agccgtgaca aacctcgtct
    15481 ttttgcagta aaaaattgtc cacggtggta tggagaaaaa acaagaacgt tttacatgac
    15541 gaaaggacta cagcgaaata ttttttatat g
//
LOCUS       DACADF010000112        15214 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.114, whole genome shotgun sequence.
ACCESSION   DACADF010000112 DACADF010000000
VERSION     DACADF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.114"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..366
                     /locus_tag="ISB30_003396"
     CDS             <1..366
                     /locus_tag="ISB30_003396"
                     /inference="COORDINATES: protein motif:HMM:NF016200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HAP1990997.1"
                     /translation="LSIDELSRLLFVWLTFIGAIVAYMDNSHVQVTFLVEKLSPANQQ
                     RVSLLTHTLILLLCLGLAWGAIEKTAQDWSNLSPILGVPVGLMYAAAIPTSLIIALLE
                     LRHLYRQFTNTPSRNQQGA"
     gene            171..1646
                     /gene="yiaN"
                     /locus_tag="ISB30_003397"
     CDS             171..1646
                     /gene="yiaN"
                     /locus_tag="ISB30_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HAP1990998.1"
                     /translation="MSGAGLGRYRKNSARLEQLIAHSWRPRWLNVRRRYPHQSDYRIA
                     GIASLIPAVHKYPQPKPTRSLTMTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMD
                     IFDVQILAQTLVNGADSFSLLAIPFFVLAGEIMNAGGLSQRIVDLPMKLVGHRPGGLG
                     YVGVLAAMIMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPP
                     SIPLIIFGVSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVW
                     QSLVSGIWALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVL
                     INAAKTTSVVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVI
                     STVMDLTPTVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGV
                     AKLKFDDAVKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            1659..2645
                     /gene="yiaO"
                     /locus_tag="ISB30_003398"
     CDS             1659..2645
                     /gene="yiaO"
                     /locus_tag="ISB30_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HAP1990999.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRQPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            2649..4145
                     /gene="lyxK"
                     /locus_tag="ISB30_003399"
     CDS             2649..4145
                     /gene="lyxK"
                     /locus_tag="ISB30_003399"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HAP1991000.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRSLLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSRGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GALFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTRVYRDFSE
                     AQRDLQHPVRTLLPDMAAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            4142..4804
                     /gene="ulaD"
                     /locus_tag="ISB30_003400"
     CDS             4142..4804
                     /gene="ulaD"
                     /locus_tag="ISB30_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP1991001.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QNCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRSLAGAANPAQVAADFHAQIDSIWGGK
                     RA"
     gene            4797..5657
                     /locus_tag="ISB30_003401"
     CDS             4797..5657
                     /locus_tag="ISB30_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP1991002.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMLETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            5651..6346
                     /gene="araD"
                     /locus_tag="ISB30_003402"
     CDS             5651..6346
                     /gene="araD"
                     /locus_tag="ISB30_003402"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP1991003.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6378..7193)
                     /locus_tag="ISB30_003403"
     CDS             complement(6378..7193)
                     /locus_tag="ISB30_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1991004.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEDERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVADPQVLVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            7419..8819
                     /locus_tag="ISB30_003404"
     CDS             7419..8819
                     /locus_tag="ISB30_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1991005.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSSPA
                     YQTLWLVVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAMAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            8830..10803
                     /locus_tag="ISB30_003405"
     CDS             8830..10803
                     /locus_tag="ISB30_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HAP1991006.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLTDHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCIQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQVDN
                     GESLHNLWLPADAPFTTFEGNGLFRHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQILTFIPWFSWANRGEGEMRIWVNEETSAIRR"
     gene            complement(10980..12518)
                     /locus_tag="ISB30_003406"
     CDS             complement(10980..12518)
                     /locus_tag="ISB30_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAP1991007.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSYGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(12626..13921)
                     /locus_tag="ISB30_003407"
     CDS             complement(12626..13921)
                     /locus_tag="ISB30_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAP1991008.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            complement(14051..15202)
                     /gene="yiaY"
                     /locus_tag="ISB30_003408"
     CDS             complement(14051..15202)
                     /gene="yiaY"
                     /locus_tag="ISB30_003408"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAP1991009.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         3488 a         4077 c         3981 g         3668 t
ORIGIN      
        1 ttatcgattg atgaattatc ccgactttta tttgtctggt taacctttat tggcgcaatc
       61 gttgcttata tggacaacag ccatgtgcag gttacttttt tagtcgaaaa actctcaccg
      121 gctaatcaac aacgtgtttc gctgctaacc catacgctca ttttattgct gtgtctgggg
      181 ctggcctggg gcgctataga aaaaacagcg caagactgga gcaacttatc gcccattctt
      241 ggcgtccccg ttggcttaat gtacgccgcc gctatcccca ccagtctgat tatcgcattg
      301 ctggaattgc gtcacttata ccggcagttc acaaataccc ccagccgaaa ccaacaagga
      361 gcctgacgat gacaatcctt atatttattg tcagtttgtt aggggctatc gcaattggtg
      421 ttcccatcgc ctgggcatta ttgttgtgcg gcatctcatt gatgttctgg atggatatct
      481 ttgatgtgca aattctggca caaacgttgg taaatggcgc agacagcttc tccctgttag
      541 ccatcccctt ctttgtactg gcgggtgaga tcatgaacgc gggcggcctg tcgcaacgta
      601 tcgtcgactt gccaatgaaa ctggtaggcc acaggccagg cggtctgggt tacgtgggcg
      661 tgctggcagc gatgatcatg gcaagcctgt ccggttctgc ggttgctgat accgcagctg
      721 tcgcggcgct gctggtgccg atgatgcgcc aggccaacta tccggtaaac cgcgccgccg
      781 ggcttatcgg ttccggcggg attattgcgg ccattatccc accttccata ccactcatta
      841 tcttcggcgt ctccagcgga ctctccatca gtaagctgtt tatggcgggc atcgcaccgg
      901 gcatcatgat gggcgtcacg ctgatggtta cctggtggtg gcaggcaaaa cgactgaatt
      961 taccttgtca gccgaaagcc tcactgcgag aagtctggca atccttagtt tccggtattt
     1021 gggcgctctt tttaccgata attattattg gcggtttccg ttcggggtta tttacaccga
     1081 ccgaagcagg cgcggttgcc gccttctacg ctctgttcgt ctcggtggtg gtctatcgcg
     1141 agatgacctt ctccacgctt tatcatgtgc tgattaacgc ggcaaaaacc acatccgtgg
     1201 tgatgttcct ggtggcctct gctgcggtat ctgcatggct tattacgatc gccgaattac
     1261 ccatgatggt atcggagcta ttacaaccgt tggttgattc gcctcgctta ttattcatcg
     1321 tcattatgct cgctattatg gtgatcagta cggtcatgga cttaacgcct accgtattaa
     1381 ttcttacacc agtattaatg ccattagtga aagaagcggg aatagatccg gtctatttcg
     1441 gcatcatgtt tattatcaac tgttcaatat cactgattac gcctccagtc ggtaatgttc
     1501 ttaacgtggt atgcggcgtg gcgaaactta aatttgacga tgcggtaaaa ggcgttgcgc
     1561 cttacgtcat ggttttattc atgttgttag cattatttat ttttataccg gagctcatta
     1621 ccgctcctct taaatggatg agttaaaagg aaatcataat gaaattacgt tctgtaacct
     1681 acgctttatt aattgccgga gtggcgacgt tcagcacatc ttctctggcg gcacaatcat
     1741 tacgtttcgg ttatgaaacc tcacaaaccg attcacaaca tattgcggcg aaaaaattta
     1801 atgaattact gcaggaaaaa accaaaggcg aactgaaatt aaaactgttc ccggatagca
     1861 cccttggtaa tgcgcaggcg atgatcagcg gcgtgcgtgg cggcactatt gatatggaga
     1921 tgtctggctc taacaacttt acgggactct cgcccgtcat caacctgctc gatgtcccgt
     1981 tcctgttccg cgataccgct cacgcgcata aaacgctcga cggcaaagtc ggtgatgacc
     2041 tgaaagtctc ccttgaaggt aaagggctga aagtgctggc ttactgggaa aacggctggc
     2101 gcgatgtcac caactcacgc cagccagtta aaacacctgc cgacctgaaa ggactaaaaa
     2161 tccgcaccaa caacagcccg atgaatatcg ccgcatttaa agtttttggc gctaacccga
     2221 tcccgatgcc gtttgccgaa gtctataccg ggctggaaac ccgcactatc gacgctcagg
     2281 aacacccgat caacgtcgtc tggtcagcaa aatttttcga agtgcagaag tacctttctc
     2341 tgacccatca cgcctattcc ccacttctgg tggtgatcaa caaagcgaag tttgatggct
     2401 taaccccgga gttccagcag gcactgatct catctgcgca ggaagcgggt agctatcagc
     2461 gcaaactggt cgccgaagat cagcaaaaaa tcatcgacgg tatgaaagaa gcgggcgtgg
     2521 aagtcatcac cgatctcgac cgcaaagcct ttagcgatgc gctgggtacc caggtccgtg
     2581 acatgtttgt taaagatgtt ccccagggtg ctgatctgct gaaagccgtg gatgaggtgc
     2641 aataaaccat gacgcaatac tggctggggt tagattgtgg cggtagctgg ctgaaagccg
     2701 ggctgtatga ccgagaaggc cgggaggcgg gcgtgcagcg cctgccgctg tgcgcattaa
     2761 gtccacagcc aggctgggca gagcgcgaca tggcagaact gtggcaatgt tgcactgctg
     2821 tcattcgctc cctgcttacc cattctggcg tcagcgggga acaaattgtc ggtatcggca
     2881 tctccgcgca gggaaagggc ttatttctac ttgataaaaa cgacaaaccg ctcggtaacg
     2941 ccattttgtc ctcggatcgc cgggcgatgg aaatcgttcg tcgctggcag gaagatggca
     3001 tcccggaaaa actctatccg ctgacccgac aaaccttatg gaccgggcat ccggtgtcgc
     3061 tgttacgctg gctgaaagag cacgaaccag aacgctacgc gcaaattggc tgcgtgatga
     3121 tgacgcacga ctacctgcgc tggtgtttaa ccggcgtcaa aggttgcgaa gagagcaata
     3181 tttctgagtc caatctctac aacatgagtc gcggggaata tgacccgtgc ctaaccgact
     3241 ggctggggat cgctgaaatc aatcacgccc tgccgcctgt tgtcggatct gccgaaatct
     3301 gcggggaaat caccgctcag acagccgtac tgactggtct gaaagcgggt acccccgttg
     3361 tcggcggcct gtttgatgtg gtttccaccg cactctgcgc cgggctcgaa gacgaattca
     3421 ccctcaatgc ggtgatgggg acctgggcgg tgaccagcgg cataacccac ggtttacgtg
     3481 acggtgaagc gcatccgtat gtctatggtc gctacgttaa cgatggtcaa tttatcgttc
     3541 acgaagccag ccccacctct tccggcaatc tcgaatggtt taccgcacag tggggagaaa
     3601 tctcatttgc tgagattaat caggccgtag ccagtttgcc gaaagccggt ggcgctctct
     3661 ttttcctgcc gttcctgtac ggcagcaacg ccggactcga aatgaccagc ggtttctacg
     3721 ggatgcaggc cattcacacc cgtgcacacc tcttacaggc tatctatgaa ggggtggtgt
     3781 tcagccatat gacccatctc aaccggatgc gcgaacgttt tactgatgtg cacaccctgc
     3841 gcgtcactgg cggcccggcg cactccgatg tctggatgca aatgctggct gacgtcagcg
     3901 gtctgcgtat cgagctgccg caggtggaag aaaccggctg ctttggtgcg gcacttgccg
     3961 cccgcgtcgg taccagggtt tatcgcgatt tcagcgaagc ccaacgtgac ttacagcacc
     4021 cggtacgcac cctgctgccg gatatggccg cccatcagct ttaccaacaa aaataccaac
     4081 gctatcaaca tctcattgcc gcacttcagg gttatcacgc ccgcatcaag gagcacacat
     4141 tatgagtcga ccacttctgc aactggccct cgaccactca tcacttgaag ccgcgcagcg
     4201 cgacgtgacg ctgttaaaag acagcgtcga tattgtcgaa gcaggcacca ttctctgttt
     4261 aaacgaaggg cttggcgcag tgaaagcctt gcgcgaacag tgcccggaca aaatcatcgt
     4321 tgccgactgg aaggtcgccg acgcgggtga aacgctcgcg caacaggcat ttggcgcagg
     4381 cgctaactgg atgaccatca tctgcgccgc gccgctcgcc acggtggaaa aaggccacgc
     4441 gatggcacag aactgcggcg gtgaaattca gatcgagcta ttcggcaact ggacgctgga
     4501 cgatgcccgt gactggcatc gtattggtgt acgacaggcg atttatcatc gtggtcgtga
     4561 cgcgcaggcc agcgggcaac agtggggcga ggccgatctg gcacgcatga aggcgctttc
     4621 cgatatcggc cttgagcttt ccattaccgg cgggattacc cctgctgacc tgccgctgtt
     4681 taaagagatc cgcgtgaaag cgtttattgc cggacggtcg ctggcaggcg cagcaaatcc
     4741 ggcgcaagtg gccgctgatt ttcatgcgca aatcgactcc atctggggag gcaagcgtgc
     4801 gtaatcatcc gttaggtatt tatgaaaaag cgctggcgaa agatctctcc tggccggagc
     4861 ggctggtgct ggcaaaaagc tgtggttttg attttgtcga aatgtcggtg gatgaaaccg
     4921 acgaacggtt gtcacgtctt gactggagcg ccgcacaacg tgcttcgctg gtcactgcga
     4981 tgctcgaaac gggtgttgct attccgtcaa tgtgcctgtc cgcacatcgt cgttttcctt
     5041 ttggtagccg ggacgacgcc gtgcgccagc gggcgcggga gattatgagt aaagcgattc
     5101 gcctggcacg cgatctcggc attcgtacta ttcaactggc aggttacgac gtctattacg
     5161 aagatcatga cgaaggcacc cagcaacggt ttgctgaagg gctggcgtgg gcggttgaac
     5221 aggcagcggc atcacaagtc atgctggcgg tggagattat ggacaccgcg tttatgaact
     5281 ctatcagcaa atggaaaaag tgggacgaaa tgcttgcgtc accgtggttc accgtttacc
     5341 cggacgtcgg caatctcagc gcctggggca atgatgttcc cgccgaactg aaactgggca
     5401 tcgaccgtat cgccgccatc cacctgaaag acactcaacc cgttaccgaa catagccccg
     5461 gacagttccg cgatgtgccg tttggcgaag gctgtgtcga ttttgttggc atcttcaaaa
     5521 cgctgcatga gctgaactat cgcggttctt tcctgattga gatgtggacc gaaaaagcca
     5581 aagagccggt gctggagatt attcaggcgc ggcgctggat tgaagcgcgt atgcaggagg
     5641 ccggatttat atgttagagc aactgaaagc tgacgtgctg gcggcaaacc tggcgcttcc
     5701 cgttcatcat ctggtgacgt tcacctgggg caatgtcagc gcgatagacg aaacgcggca
     5761 atggatggtg attaagcctt ccggcgttga gtatgaggta atgaccgccg acgatatggt
     5821 ggtggttgag atagccagcg gcaaggtggt ggaaggcagc aaaaagccct cttccgacac
     5881 gccaacgcat ctggcgcttt accgtcgctt tgcggagatt ggcggcatag tgcataccca
     5941 ctcgcgccac gccaccatct ggtcgcaggc cggacttgat ctccccgcct ggggcactac
     6001 tcacgccgat tatttttacg gagccatccc ctgcacgcgc ctgatgaccg cagaagagat
     6061 caacggtgag tatgaatacc agaccggcga agtgatcatt aaaaccttcg aagagcgtgg
     6121 gttgaacccg gcacaaatcc cggcggtact ggtgcattct cacggcccgt tcgcctgggg
     6181 aaaaaatgcc gccgatgcgg tgcataacgc cgtggtactg gaagagtgcg cctatatggg
     6241 tctgttctcg cgccagcttg cgccacagct ccctgcgatg caaaacgaac tgctggataa
     6301 gcactacctg cgtaagcatg gggccaatgc ctattacggg cagtaatccc tcacgccggg
     6361 gcttcatcgc cccggcacta cgaattgata tgttccttgc tgtaacgccg cttccacgct
     6421 gctggcgtta agccggtgtg tttgcgaaaa atctgccgga aatagccgac gtcattaaac
     6481 ccgcacaacg ccgccacttc cgtgagcgat cgcgcttcgc taattaacag cttttcggct
     6541 tcgcgcaccc gctggcggtg gattgcttct gttaacgtca gatgaaacac ccgccgatag
     6601 acgcgcccaa gataatcggc attgcagtac agctcgcggg ctaaaagcgc cgtggaaagc
     6661 gggcggtgat attgcgtgcg aatcagtttc tgcgctttcc aggcaagggt ggtgcctgca
     6721 ccatcggcct gttcatcaag acgcgcgtca cgggtgagct gctgcaaaat cagcagcaca
     6781 ataaactcca gcgccggagt gcgttgatgc ttttcctgtt cgcttaagaa ctggcgaaac
     6841 aaggccacca gcacctgcgg atcggcaacc ttagtttgtt gcggcacgct aagatgagtg
     6901 agccagcgcg gatcgttgtc cggcgcttta acctcaaaat gcagccagta aaatttgaga
     6961 tcggcgggaa aatcctccac cccaacatgc tgtcggttcg gccagagtaa taaactctct
     7021 cccgcatcga ccagaaatat ccgttcatct tcctgaatcg ttaatcgacc tttttcgacg
     7081 aagataattt cccatgactc cagccgcctt gcaggatgcc tccctacgcc acgggaaata
     7141 aataatccac cattttgcac tttaatcggt aacgtcatgg ataattcgag cattgatatg
     7201 taatttcccg cttattcatt aatgattaaa cacattacct tttgattatt aatcaaaaac
     7261 attaatttca cctgatccat ttcacacttt taaattcagg tcggaatcgt cacctttttt
     7321 tactcttttc tcgccttgtt gatattcccg ttcacggcag aattaatcag gtcattgcaa
     7381 taaaactaaa aattgctccc tattcacgag gaatatctat gacctctaca ccgattacca
     7441 ccgctgatat cgctaaaggt aaaattgacg atgcgttatc tgtacgggaa aaaataggct
     7501 acggcctggg tgacgcaggc ggcaccgtaa taacttgcct gatcatgaac tttctcacct
     7561 ttttctacac cgacgttttt ggcttaactc cggcgctggt tggcacgctg tttattgcgc
     7621 tacgcgtgtt tgatgccatc tccgacccgg tgatgggcgt cattgccgac cggacgcaaa
     7681 gtcgctgggg gcgctttcgt ccgtggcagt tatggattgc cattcccatc ggcattatcg
     7741 gcatcctgac attcaccgtg ccagatgcca gcatgggagt aaaaatcgcc tgggcgttcg
     7801 gtacttacct gctcctttca gtcggttata cagccatcaa cgtaccgtac tgcgcgctga
     7861 tcaacaccat gaccacccgc cacaatgaag tgatctcctg ccagtcctgg cgattcgttc
     7921 tctgcggcgt agcgggattt ctggtttcgg taggcttacc gtggatggta gatctcttcg
     7981 gtcagggcaa cgctgcacgc ggctatcaac ttggcgtcgg cgtattgtgc gccattgccg
     8041 tggtgatgtt cctgtgctgt ttcttctggg ttcgtgaacg ggtgccactc tccacaatgg
     8101 ggaaatttac cctgcgcgaa catcttgccg ggctgcggaa caacgaccaa ctgctgctga
     8161 tgctggtcat gtctttcctg ctgattaacg tctttaatat tcgcggcggt gggtatatgt
     8221 acttcattac ctacgtctta caaggcagta cgggctacac gtcgctgttc ttcaccatgg
     8281 tcaccttcgc ctccattatc ggctcggtga ttgtcagccc attaacgcgg cgtttcgata
     8341 ccgtcaaaat ttactactac accaacctgc tcctcgctgc actggcggtg ttgatgtggt
     8401 tcctgccctc cagcccggct tatcaaacgc tgtggctggt ggtgatcctc ggtaatggcg
     8461 tgattcttgg cttcacattg ccactgcact tctcattgat ggcctttgcc gatgactacg
     8521 gcgagtggaa aacccgcgta cgttcttccg gcatgaactt cgccttcaat ctgtttttca
     8581 tcaagctggc ctgggcctcc agcgccggga tcatcagcct gctgtttatt tttgtcgcct
     8641 accagccagg cgtggaaaac cagaccgcca gttcgcttgg cgggatcgcg gcgatggaaa
     8701 cattgctgcc tgcgctgttc cacctgctgc tggcgatggc gatccgcttt tgcaaactca
     8761 ataaccctat gatgtcacgc attgctagcg acctgcgtca gcgtcatgta cagccttaag
     8821 gagcacaaga tgaacatttc ggaagtcgat ctgcataaac tgacggtcag cgatccgttc
     8881 ctcggtcagt accaacaact ggtccgcgac gtggtgattc cttatcagtg ggatgccttg
     8941 aacgatcgta tcccagaagc ggaacccagc catgcgattg aaaactttcg cattgccgcc
     9001 ggacttcagg agggtgaatt ttacgggatg gtgtttcagg acagcgacgt cgccaaatgg
     9061 ctggaagcgg tagcctggtc gctgtgccag aagccggacg ccgaactgga aaaaaccgcc
     9121 gacgaggtga ttgaactgat cgcttccgcc cagtgcgaag atggctatct caatacttac
     9181 tttacggtaa aagcgcccga agaacgctgg agcaatctgg cggagtgtca tgaactttac
     9241 tgcgccggtc atctgattga agccggagtc gccttcttcc aggctacggg caagcggcgc
     9301 ttgctggaag tggtttgccg tctgaccgat catatcgaca gcgtatttgg tccagatgaa
     9361 agtaagttac atggttatcc tggtcacccg gaaattgaac tggcactaat gcgcctgtat
     9421 gaagtgaccg aagagccgcg ctacctggcg ctgacgaact attttgtcga acagcgtggt
     9481 gcgcaaccgc actattacga ccaggaatat gaaaagcgcg ggcagacctc gcactggcac
     9541 acctacggcc cggcgtggat ggtgaaagac aaagcctaca gccaggcaca tttgcccatc
     9601 gcacagcagc aaaccgccat tggtcacgcg gtacgttttg tctacctgat gaccggcgtc
     9661 gcgcatctcg cgcgtttaag tcacgatgaa agcaagcgtc aggactgcct gcggctgtgg
     9721 aacaatatgg cccagcgtca gttatatatt accggcggca ttggctcaca gagcagcggt
     9781 gaagcgttca gcagcgatta cgatctgccg aatgacacgg tatacgctga aagttgcgcc
     9841 tccatcggcc tgatgatgtt tgcccgacga atgctggaaa tggaaggcga cagtcaatat
     9901 gccgatgtga tggagcgcgc actgtacaac accgtgctcg gcggcatggc gctggatggc
     9961 aaacatttct tctatgtgaa tccgctggaa gtacatccaa aatcgctgaa attcaaccat
    10021 atctacgatc acgttaagcc gatccgccag cgttggtttg gctgcgcttg ttgtccgcca
    10081 aatatcgccc gcgtgctaac ctcgattggt cattatctct acacgccgcg tgaagatgcg
    10141 ttgtatatca acatctacgc aggaaacagc atggaggtgc cggtagaaaa tggcacgctg
    10201 cgcctgcggg ttagcggaaa ctatccgtgg caggaacagg tgacgattgc ggttgaatca
    10261 ccccagccgg tgcgtcatac gctggcttta cgtctaccgg actggtgcat acagccgcag
    10321 atcatattga atggggaaga ggtcgagcag gatattcgta aagggtattt gcacattacc
    10381 cgtgaatggc aggagggcga cacgctgaat ctgactttgc caatgccggt acgtcgcgtt
    10441 tacggtaacc cgctggtgcg tcacgtcgcc ggaaaagtgg cgattcagcg cggcccgctg
    10501 gtgtattgtc tggaacaggt cgacaacggc gagtcactgc ataatctgtg gctgcccgcc
    10561 gatgcgccat ttacgacatt tgaaggtaat ggattgttcc gccataagat cttaatccag
    10621 gcaccgggtt accggtatga acagagcaat ccagagcagc aaccgctgtg gcattacgac
    10681 agcgcgccag ccaaacgcca gccgcaaatt ctgacgttta tcccgtggtt tagctgggcc
    10741 aaccggggtg aaggcgaaat gcggatctgg gtgaatgagg aaaccagtgc catccgtagg
    10801 taggataagg cgctcgcgcc gcatccaacg catggtgcac attgccggat gcggcgtgaa
    10861 cgctttatcc gacctacaaa agcatgcaaa ttcaatatat tgcatgagct atgtaggcat
    10921 gataagacgc gaaagcgtcg catcaggcaa tgaatacccg atgcgaccag cttcttacat
    10981 cagaacagcc ccaacggttt atccgagtag ctcaccagca ggcacttggt ttgctggtaa
    11041 tgctcaagca tcattttgtg ggtttcacgg ccaatgcctg attgtttgta gccgccaaac
    11101 gccgcatgtg ccgggtaagc gtgataacag ttggtccaca cgcgccctgc ctgaatgccg
    11161 cgccccatct tataggccag attaccgttg cggctccaga cgcccgcgcc cagaccatat
    11221 tgcgtatcgt tcgccagctc cagcgcttct tccatcgttt tgaaggtggt taccgccagc
    11281 accgggccaa aaatctcctc ctggaacacg cgcatattgt tctgaccaaa cagaatcgtc
    11341 ggttcgaggt agtagccgtc tttcagttcg ccttccagca gcttacgtcg cccgccggtg
    11401 agcacatcag cgccctcttt tttaccgata tcaatgtagt tgaggatggt ttccagttgc
    11461 ccgtaagaaa cctgcgcgcc catttgcgtc acgctgtcga gcgggttacc gctgcgaatg
    11521 ctttcgacac ggcggatagc gcgttccata aagcgttcgt agatagactc ctgcactaaa
    11581 gcacggcttg gacaggtgca aacttcgccc tggttaaagg caaacagtgc aaagccttcc
    11641 agcgctttat cgaaaaaggc atcttcttca tccatcacat cagcaaagaa gatatttggc
    11701 gatttgccgc ccagctccag cgtcaccgga ataatgtttt gcgtggcgta ttgcataatt
    11761 tgttggccca cttccgttga gccggtaaac gccactttgg cgatgcgttt cgaggtcgcc
    11821 agatattcgc caatttcccc acctgcgcca ttgaccacgt tcaccacccc tggcggcagt
    11881 aaatcaccga cgatttccat tagcagcagt acagaaagcg gagtaagacg tgcgggtttc
    11941 agcaccacac agttgcccgc cgccagcgcg ggggccattt tccagctcgc catcagcagc
    12001 gggaagttcc acgggataat ctgccccacc acgcctaacg gttcatggaa atgataggcc
    12061 acggtttcgc tatccacttc actgatccca ccttcctgcg cccgaataca cgaggcgaaa
    12121 tagcgaaaat gatcaatcgc cagcggcacg tcggcagcac tggtttcgcg aatgggtttg
    12181 ccgttatccc aggtttctgc cgtcgctaaa agttcaaggt tttgttccat tcgatcggca
    12241 atcttaaaca gaatcgccgc gcgatcctgc accgaggtgt gcgcccattt atctttcact
    12301 ttatgcgcag catccagcgc cagatcgatg tctcgtttgc ccgaagacgc cacttcgcac
    12361 agtagctgcc cggtcaccgg cgtcaggttc tggtaatact cgccatcggc aggcgctacc
    12421 cattcgccac cgataaagtt gtcgtagcgg gcttttaatt tgagggggaa accatactcg
    12481 ccgggcttaa tctgtgctga agggggatta ttggtcatga tcgtctcctt gcggtgtgag
    12541 gtataacaag ggtagacgtg actggcgaaa tcttcgccag tcggtaacag ctttacgacg
    12601 gctatcacga atttacgggc aaaagctaca gcgggtagtg tgcgacgtac actgcttgct
    12661 ggttattaat tcttttctct tgcgcgaatc cttctttaac aattgtccgt aaatcagctc
    12721 gagccgtatt atcagagacg cccaagctcg cgctaatttc agcaactgtg tactctttcc
    12781 ccggattggc gagcatcaca ttcaggagcg ttacctggcg cggattcagg cgtttaagca
    12841 caccagagct aaacagcaca ctatcgagtt ttgcgcttcg ttgtgtaatt tcatcgatat
    12901 gctgctgcca gttttgcaga gcacgtttaa tgacacccgc ctgatattca aggaaataag
    12961 tcagatccat accatctgat tcagtgtact ggtagctggc ggcatatttg accggtgcag
    13021 catgaagcaa acggctgatg gaaatatatt taaagacatc gtagcccgat ttcagcatat
    13081 accagtaaaa cagcgcccga ctggtgcgcc cgttgccatc ccgaaacgga tgttcatggg
    13141 ccagcataaa atgcagaatg caggcacgga ccaacggatg aatataccct tcatggctgt
    13201 ttaaccactg gcaaactttt tccagccgtt ccggtaacaa tgccgcagcg ggcggttgat
    13261 gcactatatt gccatcatag tcggcgatca caacatcatc tgtttcacga aattctcccg
    13321 gacgatattt cgcgtcattg attccaccca taccaatggc atgtaactgg cgaataagcg
    13381 caggtgtcag cggttctgcg agtaagtgcg gaatttccgc catcagccgg gcatttcccg
    13441 caatcatatg ctcgtcttca gtgcgtggcg accgccctgt ttccagcatt tttctcgcca
    13501 ccagcgttgt agtattcgca ccttccagct gggagctggt gatcggctct tccaggcgta
    13561 actggcttaa ctcggctccg gccccgcgta accgttcagt ctgatctttc agcgccagcg
    13621 cggtagcacg tttgtcgaca agctcgcacg cttctgcaat caccggggta atgttgaatc
    13681 cggcctgttc acccgcttca ttgcaataat taattcgttg gatcgccgcg ttgcgcgcca
    13741 gacgggtaag cgtccacgcc acatcgacgt tttctccttt tttgatgcgg tactgcaact
    13801 catcaaaagg cagataacgt cctttttcat cggtaacccg gttaaagcgc aggtgggcca
    13861 ggagttcctc acccgataag tgtgataaag gaataagtcc gggaggtgat ttgatagcca
    13921 tgtgcttatc tcaaaaaagc gcaatattga gatagtgtat agcaggaaag agaaactatc
    13981 tcaaaaaaag caagttttga gatagttgaa aaagcggctg acaactcgcc agccgttgtg
    14041 ggaatgatga ttacatcgct gcgcgataaa tcgccacaat ttcttcgtga gttgcctgga
    14101 tcgggttagt aaagccgcag gcatctttca gggcattagt cgccagaacc gcgaaatctt
    14161 cttctttcac gttcaggtcg cgtaggcctg ccgggatatc cactttcttc gccagttcac
    14221 ggatggcgtt aatgcaggct tcagcacctt ccgcgtcgtt tttacctgtc acgttcacgc
    14281 ccattgcagc ggcacaatca cgcagacgtg cagcggcgac tttgctgttg aatacctgaa
    14341 catgcggcag caaaacggcg ttacatacac cgtgtggcag gttgtagaaa ccgcccagct
    14401 ggtgcgccat cgcatgaaca taacccagag aagcattatt gaacgccata ccggcgagga
    14461 actgggcata agccattgct tcacgcgctt tcgcattact gccatcttca acggctaacg
    14521 gcaggttttc ggcaatcatg gtcacggctt tcagtgcaca agcgtcagtg atcggcgtgg
    14581 cggcaataga aacatacgct tcgatagcgt gcgttaaggc atccataccc gttgcggcgg
    14641 tcagtgactt cggcataccg atcatcagag aggagtcatt gacagaaagc agcggagtga
    14701 catgtttatc cacaatcgcc attttgatgt gacgcgcttc gtcagtgatg atgcaaaaac
    14761 gggtcatttc agaggccgta cccgccgtgg tgttgatggc gatcatcggc agctgcggtt
    14821 ttgcagagcg gtcaacgcct tcataatcgc ggatgtcacc gccgttggct gcaaccagcg
    14881 caataccttt tgcgcagtcg tgtggagaac caccgcctaa ggaaatcacg ctatcgcaat
    14941 tattttcttt aagtaatttc aaacctgcag cgacgttttc cgtggtggga ttaggttggg
    15001 tgccatcata aataacgcta aaaatattgc gttcttccag tgctttttgc acatcacccg
    15061 ccatacccaa tttcgttaac atattgtcgg tgacaattaa ggtacgggta aatccataat
    15121 ctgccatcat attcattgca tcattcaatg aatcagcacc aataacattc acggaaggaa
    15181 taaaaaaggt tgaagctgcc ataacacact cctg
//
LOCUS       DACADF010000113        15160 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.115, whole genome shotgun sequence.
ACCESSION   DACADF010000113 DACADF010000000
VERSION     DACADF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.115"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            23..577
                     /locus_tag="ISB30_003409"
     CDS             23..577
                     /locus_tag="ISB30_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAP1991010.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMANYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLSAH"
     gene            654..872
                     /locus_tag="ISB30_003410"
     CDS             654..872
                     /locus_tag="ISB30_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAP1991011.1"
                     /translation="MMPRQPGPPSSTGIGCGCGSDNAGEGVDQDYKQAMYWYLKAAAQ
                     ESVGAYVNIGYMYKHGQGVEKDYQAAFE"
     gene            complement(1122..1604)
                     /locus_tag="ISB30_003411"
     CDS             complement(1122..1604)
                     /locus_tag="ISB30_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAP1991012.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1839..4421
                     /gene="leuS"
                     /locus_tag="ISB30_003412"
     CDS             1839..4421
                     /gene="leuS"
                     /locus_tag="ISB30_003412"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP1991013.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4436..5017
                     /gene="lptE"
                     /locus_tag="ISB30_003413"
     CDS             4436..5017
                     /gene="lptE"
                     /locus_tag="ISB30_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAP1991014.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5017..6048
                     /gene="holA"
                     /locus_tag="ISB30_003414"
     CDS             5017..6048
                     /gene="holA"
                     /locus_tag="ISB30_003414"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP1991015.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMSEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6050..6691
                     /gene="nadD"
                     /locus_tag="ISB30_003415"
     CDS             6050..6691
                     /gene="nadD"
                     /locus_tag="ISB30_003415"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP1991016.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6715..7326
                     /locus_tag="ISB30_003416"
     CDS             6715..7326
                     /locus_tag="ISB30_003416"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAP1991017.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQQAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7586..7903
                     /gene="rsfS"
                     /locus_tag="ISB30_003417"
                     /gene_synonym="rsfA"
     CDS             7586..7903
                     /gene="rsfS"
                     /locus_tag="ISB30_003417"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP1991018.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7907..8374
                     /gene="rlmH"
                     /locus_tag="ISB30_003418"
     CDS             7907..8374
                     /gene="rlmH"
                     /locus_tag="ISB30_003418"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP1991019.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8405..10306
                     /gene="mrdA"
                     /locus_tag="ISB30_003419"
     CDS             8405..10306
                     /gene="mrdA"
                     /locus_tag="ISB30_003419"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAP1991020.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10309..11421
                     /gene="mrdB"
                     /locus_tag="ISB30_003420"
                     /gene_synonym="rodA"
     CDS             10309..11421
                     /gene="mrdB"
                     /locus_tag="ISB30_003420"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAP1991021.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11432..12520
                     /gene="rlpA"
                     /locus_tag="ISB30_003421"
     CDS             11432..12520
                     /gene="rlpA"
                     /locus_tag="ISB30_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAP1991022.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12660..13871
                     /gene="dacA"
                     /locus_tag="ISB30_003422"
     CDS             12660..13871
                     /gene="dacA"
                     /locus_tag="ISB30_003422"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAP1991023.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13981..14244
                     /locus_tag="ISB30_003423"
     CDS             13981..14244
                     /locus_tag="ISB30_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAP1991024.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14345..14986
                     /gene="lipB"
                     /locus_tag="ISB30_003424"
     CDS             14345..14986
                     /gene="lipB"
                     /locus_tag="ISB30_003424"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAP1991025.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDESTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3763 a         3866 c         3930 g         3601 t
ORIGIN      
        1 aagaaaatcg aagcattcac acatgaataa aaaattaatg tatatattcg caatatttat
       61 agttgcagca ataacctgta ttagccaacc caagaaaacg acgttgcgtg ataaagccat
      121 ggcgaattat gcctttgatt atttaagctc accgggcagt cttccattca ccacggcagc
      181 cacggagctt tccgcgattc atggtcactc aacgtcgcaa tatcgccttg gagaatttta
      241 tcttcatggt agcgacggta aaccactgga ttatacacag gcgagatact ggtatgagca
      301 atcagcagaa caggaaaatc cacgcgcgca aagtaaactg gggtggatct acctcaaagg
      361 tctgggggtc aaacccgaca cccgtaaagc aattctctgg tataaggaag cagctgaaca
      421 agggtatgct catgctcaat atactttggg tttgatctac agaaatggct caggtattaa
      481 tgttaaccat tatgaatctc aaaaatggtt aaaactggcc gccaaacaac attacaaaaa
      541 tgcggaaaga ttacttgccg ggctttccgc acattaacgt cgactgtcac ccgaacagat
      601 ggagtatttt ccctatcaaa ccagcaaaga taaaaatata gaaaataatt ttaatgatgc
      661 ccagacagcc tggcccacca tcgtcaacgg gcattggctg cggttgcggc tccgataacg
      721 ctggcgaagg ggtggaccag gactataagc aagcgatgta ctggtatctg aaggctgctg
      781 ctcaggaaag cgtcggcgct tacgtcaaca tcggttatat gtataaacac ggacaaggcg
      841 ttgagaaaga ttatcaggcc gcctttgaat agtttacgat agccgctgaa tgcaatgacg
      901 ccacagcctg gtataacctg gccattatgt atcactacgg agaaggaaga cctgtcgatc
      961 tccgacaggc tctcgacttg tatcgtaaag ttcagtcatc cggaaccaga gatgtcagtc
     1021 aagaaattcg tgagattgaa gatttactgt agagataaaa acaagggccg gggaatttca
     1081 ttcggcccat attctcttta ttgctgtgcg aaatcagact attacggctc aaacgggcgt
     1141 ctctggaact ggtcatgacc acatttaggg catagcgtca gcacttccgg tgtgtagatc
     1201 gggagatgga agtgacattt ctcgcagacc agatttccca gccctaccac ttctccgctg
     1261 tgataaaccc catgatgatt gaggtcctgg aaaacttcgc gccattcaag ctgcgtttta
     1321 tcggtgatgt ctgccagctc ctgccacaag ctttctttaa tcacccgcat aaagacgctg
     1381 tcagactctt ctttcaggct ctcttcatag ctcatggcga actcttccag gtcacgtctg
     1441 acagctcgcg tcagctcatc gacctcggtt cgcgttaact cccctgtttt tattacgcgc
     1501 tcgcgcgcct gttccaccag tgcgtcgata tcacgttcgc cattgcgcag gcgttcgctc
     1561 agtgacgcga ccagttcacg gtaatattga gcaaccttgt tcatcgtttc atctcccggg
     1621 tcgttaactg actataataa tagacgttct ttgttccttg ctttgctaat acgcccacaa
     1681 actctgtaaa ttaatgtatg aataatccgg gcatcatttc aacgtcgagt gcgcgaaagg
     1741 ctgttttgac gcgggcgttt gggctatgct atgcggatct gaaaaaccac atcaacgcta
     1801 catttgtagc cgtattgaaa acaggaccac tggctgccat gcaagagcaa taccgcccgg
     1861 aagagataga atccaaagta cagcttcact gggatgagaa gcgcacattt gaagtaaccg
     1921 aagacgagag caaagagaag tattactgcc tgtctatgct tccctatcct tctggtcgac
     1981 tacacatggg ccacgttcgc aactacacca tcggtgacgt gatcgcccgc taccagcgca
     2041 tgctgggcaa aaacgttctg cagccaatcg gctgggacgc atttggtctg cctgcggaag
     2101 gcgcggcggt gaaaaacaac accgcgccag caccgtggac gtacgacaac atcgcgtata
     2161 tgaaaaacca gctcaaaatg ctgggctttg gttatgactg gagccgcgag ctggcgacct
     2221 gtacgccgga atactaccgt tgggaacaga aattcttcac cgagctgtat aaaaaaggcc
     2281 tggtatataa gaagacttct gcggtcaact ggtgcccgaa cgaccagacc gtactggcga
     2341 acgaacaagt tatcgacggc tgctgctggc gctgcgatac caaagttgag cgtaaagaga
     2401 tcccgcagtg gtttatcaaa atcacagctt acgctgacga gctgctcaac gatctggata
     2461 aactggatca ctggccagac accgttaaaa caatgcagcg taactggatc ggtcgttccg
     2521 aaggcgtgga aatcaccttc aacgttaacg actatgacaa cacgctgacc gtttacacta
     2581 cccgcccgga cacctttatg ggttgtacct acctggcggt ggctgcgggt catccgctgg
     2641 cacagaaagc ggcggaaaat aatcctgaac tggccgcctt tattgacgaa tgccgtaaca
     2701 ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa aggcgtcgat actggcttta
     2761 aagcggttca cccattaacg ggcgaagaaa ttcccgtttg ggcagcaaac ttcgtattga
     2821 tggaatacgg cacgggcgca gttatggcgg ttccgggtca cgaccagcgc gactacgagt
     2881 ttgcctctaa atacggcctg aacatcaaac cggttatcct ggcagctgac ggctctgagc
     2941 cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct gttcaactct ggcgagttta
     3001 atggtcttga ccatgaagcg gccttcaacg ccatcgccga taaactgact gcgatgggcg
     3061 ttggcgagcg taaagtgaac taccgcctgc gcgactgggg tgtttctcgt cagcgttact
     3121 ggggcgcgcc gattccgatg gtgacgctgg aagacggtac cgtaatgccg accccggacg
     3181 accagctgcc ggtgatcctg ccggaagatg tggtgatgga cggcattacc agcccgatta
     3241 aagcagatcc ggagtgggca aaaactaccg ttaacggtat gccagcactg cgtgaaaccg
     3301 acactttcga cacctttatg gagtcctcct ggtactatgc gcgctacact tgcccggagt
     3361 acaaagaagg tatgctggat tccaaagcgg ctaactattg gctgccggtg gatatttaca
     3421 ttggtggtat cgaacacgcc attatgcacc tgctctactt ccgcttcttc cacaaactga
     3481 tgcgtgatgc aggcatggtg aactctgacg aaccagcgaa acagttgctg tgtcagggta
     3541 tggtgctggc agatgccttc tactatgttg gtgaaaacgg cgaacgtaac tgggtttccc
     3601 cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat cgtgaaagcg aaagatgcgg
     3661 caggccatga actggtttat accggcatga gcaaaatgtc caagtcgaag aacaacggta
     3721 tcgacccgca ggtgatggtt gaacgttacg gcgcggacac cgttcgtctg tttatgatgt
     3781 ttgcttctcc ggctgatatg actctcgaat ggcaggaatc cggcgtggaa ggggctaacc
     3841 gcttcctgaa acgtgtctgg aaactggtgt acgagcacac agcaaaaggt gatgttgcgg
     3901 cactgaacgt cgatgcgctg actgaagatc agaaagcgct gcgtcgcgat gtgcataaaa
     3961 ctatcgctaa agtaaccgat gatatcggcc gtcgtcagac cttcaacacc gcaattgcgg
     4021 cgattatgga gctgatgaac aaactggcga aagcaccgac cgatggcgag caggatcgcg
     4081 ccctgatgca ggaagcgctg ctggcggtag tccgtatgct taacccgttc accccgcaca
     4141 tctgcttcac gctgtggcag gaactgaaag gcgaaggcga tatcgacaac gcgccgtggc
     4201 cggttgctga cgaaaaagcg atggtggaag actccacgct ggtcgtggtg caggttaacg
     4261 gtaaagtccg tgcaaaaatc accgttccgg tggacgcaac ggaagaacag gttcgcgaac
     4321 gtgctggcca ggaacatctg gtagcaaaat atcttgatgg cgttactgta cgtaaagtga
     4381 tttacgtacc aggtaaactc ctcaatctgg tcgttggcta agcgcgggag gaagcgtgcg
     4441 atatctggca acattgttgt tatctctggc ggtgttaatc accgccgggt gtggctggca
     4501 tctgcgtgac accacgcagg ttccttccac tatgaaagtc atgatcctgg actcaggcga
     4561 tccgaacggg ccattaagcc gtgcggtgcg taaccagtta cgtctgaatg gtgtcgagtt
     4621 gcttgataaa gaaaccacgc gtaaggacgt tccatccctg cgtttgggta aagtgagcat
     4681 cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca gcagagtatc agatgatcat
     4741 gacggtgaat gcgaccgtgt tgatccccgg ccgtgacatc tacccgatta gcgccaaggt
     4801 cttccgttcg ttcttcgata acccgcaaat ggcgttagcg aaagataacg aacaagacat
     4861 gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg attcgtaagc tgccaagcat
     4921 ccgtgctgca gatattcgtt ccgacgaaga acagacgtcg accacaacgg atactccggc
     4981 aacgcctgca cgcgtctcca ccacgctggg taactgatga ttcggttgta cccggaacaa
     5041 ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc ttttacttgg taacgatcct
     5101 ctgttattgc aggaaagcca ggacgctgtt cgtcaggtcg ctgcggcgca agggttcgaa
     5161 gaacaccaca ctttttccat tgatcccaac accgactgga atgcgatttt ttcgttatgc
     5221 caggccatga gcctgtttgc cagtcgacaa acgctattgc tgttgttacc cgaaaacgga
     5281 ccgaatgcgg cgatcaatga acaacttctc acactcaccg gacttctgca tgacgatcta
     5341 ctgttgatcg tccgcggtaa taaattaagc aaagcgcaag aaaatgccgc ctggtttact
     5401 gctcttgcga atcgcagcgt gcaggtgacc tgtcagacac cggagcaggc tcagctgccc
     5461 cgctgggttg ctgcgcgcgc aaaacagctc aacttagaac tggatgacgc tgcaaatcag
     5521 gtgctctgct actgttatga aggtaacctg ctggcgctgg cccaggcact ggagcgttta
     5581 tcgctactct ggccggacgg caaactgaca ttaccgcgcg ttgaacaggc ggtgaatgat
     5641 gccgcgcatt tcaccccttt tcattgggtt gatgctttgt tgatgggaaa aagtaagcgc
     5701 gcgttgcata ttcttcagca actgcgtctg gaaggcagcg aaccggttat tttgttgcgc
     5761 acattacaac gtgaactgtt gttactggtt aacctgaaac gccagtctgc ccatacgcca
     5821 ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc gccggggcat gatgagcgaa
     5881 gcgttaaatc gcttaagcca gccgcagtta cgtcaggccg tgcaactcct gacacgaacg
     5941 gaactcaccc tcaaacaaga ttacggtcag tcagtgtggg cagagctgga agggttatct
     6001 cttctgttgt gccataaacc cctggcggac gtatttatcg acggttgata tgaaatcttt
     6061 acaggctctg tttggcggca cctttgatcc ggtgcactat ggtcatctta aacccgtgga
     6121 aacgctggcg aatttgattg gtctgacgcg ggtcacaatc atccctaata atgttcctcc
     6181 gcatcgtccc cagccggaag cgaacagcat gcagcgtaaa cacatgcttg aactggcgat
     6241 tgccgacaag ccattattta ctcttgatga acgcgagcta aagcgcaatg ccccctctta
     6301 cactgcgcaa acactgaaag agtggcggca ggaacaagga ccggacgtgc cgctggcgtt
     6361 tattattggt caggattcat tgctgacctt tccgacctgg tacgaatacg aaacgatact
     6421 cgacaatgca catttgatcg tctgtcggcg tccaggttac ccacttgaaa tggcgcaacc
     6481 gcaataccag caatggctgg aagatcattt gacacataac ccggaagatc ttcaccttca
     6541 gcctgccggt aaaatttatc tggctgaaac gccgtggttt aacatctcgg cgaccatcat
     6601 ccgcgaacgt ttgcaaaacg gtgaatcgtg tgaggattta ttaccggaac cggtactgac
     6661 ttacattaac caacaaggct tgtatcgctg atacctgtcg ttacggaggg catgatgcga
     6721 ctgtggttaa ttcgtcatgg tgaaacgcaa gcgaatgtcg atggtctgta cagcggtcat
     6781 gcgcccacac ccctgaccgc gcgcggtatt gagcaagcgc aaaatctgca tacgctgcta
     6841 catgatgttt cctttgacct ggttttatgc agtgaactgg aacgggcaca gcataccgcg
     6901 cgactggttc ttagtgaccg ccagcacccc gttcatatca tccctgaact caatgaaatg
     6961 ttttttggcg actgggagat gcgacatcat cgtgacctca tgcaagaaga tgccgaaaac
     7021 tatagcgcgt ggtgcaatga ctggcagcaa gctatcccca cgaacggtga aggatttcag
     7081 gcattttcgc aacgtgtgga acgatttatc gcaaggctta gtgaatatca gcactatcag
     7141 aatattttag tcgtcagcca tcagggcgta ctgagtctgt taattgctcg tttaattggt
     7201 atgcctgccg agtctatgtg gcatttccgc gttgaccagg ggtgttggag cgccattgat
     7261 atcaaccaaa aattcgcgac gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaat
     7321 gcatgacttt tctgtttttt tacgggtaag ccgcaacgac cattgacagc cccgggcagg
     7381 ctgatattct ccgcagccag actttttccg ccagacacga ctttgtagaa attgttttac
     7441 aaaaatggcg atgcaatctg cggcgcgggg tgggatgata gcccactttc gaaagccgat
     7501 tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat actgacagac catttttatc
     7561 tatttgattc acccaggggg aaaacttgca gggtaaagca ctccaggatt ttgttatcga
     7621 caaaattgat gacctcaaag gtcaggacat catcgcctta gacgttcagg gcaaatccag
     7681 catcaccgac tgcatgatca tctgtacggg tacgtccagc cgccatgtta tgtccattgc
     7741 tgatcacgtt gtgcaggagt ctcgcgcagc gggcctgtta ccgctcggcg tagaaggtga
     7801 aaacagcgcc gactggattg tcgtggattt gggcgatgtg attgtccatg tcatgcagga
     7861 agagagccgt cgcctgtatg aactggaaaa actctggagt taatgcgtga agctgcaact
     7921 tgtcgccgtg ggaacgaaaa tgccggactg ggtacaaacc ggttttaccg agtacctgcg
     7981 tcgttttccg aaagatatgc ccttcgagct gattgaaatt ccggccggaa aacgcggcaa
     8041 gaatgcggac atcaagcgca tactcgacaa agagggtgag cagatgttgg cggccgcagg
     8101 caaaaaccgc attgtcaccc tcgatattcc aggcaagccc tgggatacgc cgcagttagc
     8161 cgctgagctg gaacgctgga agctggatgg tcgcgacgtc agtctactga ttggcgggcc
     8221 tgaagggttg tcgcctgcct gtaaagcggc ggcggagcag agctggtcgc tgtcggcact
     8281 tacccttccc catccgctgg ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag
     8341 catcaccacc aaccatcctt atcaccgtga gtgataagtg agctttgagt agaaaacgca
     8401 gcggatgaaa ctacagaact cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg
     8461 ccgggcgctg gtcgcctttt tggggatttt gctgctgacc ggcgtgctta tcgccaacct
     8521 gtataatctg caaattgttc gctttaccga ctaccagacc cgctctaatg aaaaccgcat
     8581 taagctggtg cctatcgcac ccagccgcgg cattatctac gaccgtaacg gtatccctct
     8641 ggctctcaac cgcactatct accagataga aatgatgccg gagaaagtcg ataacgtgca
     8701 gcaaacgctg gacgctttgc gcagcgtggt tgatctgacc gatgatgata ttgctgcatt
     8761 ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt ccggtgaaaa ctaacctgac
     8821 cgaagtacaa gtagctcgct ttgccgtcaa ccagtaccgt tttccgggtg ttgaagttaa
     8881 aggctataaa cgtcgttact atccttacgg ttcggcgttg acccacgtca tcggctatgt
     8941 gtcgaaaatc aacgataaag acgtcgaacg cctgaataat gacggcaaac tggccaacta
     9001 tgcggcaacg catgatatcg gtaagctggg cattgagcgt tactatgaag acgtgctgca
     9061 cggtcagacc ggttatgaag aggttgaagt taacaaccgt gggcgtgtta tccgccagtt
     9121 aaaagaagta ccaccgcagg ccggacacga tatttacctg acgctggatc tcaaactcca
     9181 gcaatatatt gaaacgctgc tggcgggtag ccgcgcagct gtggtagtca ccgatccgcg
     9241 taccggtggg gtgttggcgc tggtttccac gcctagttat gacccaaacc tgtttgttga
     9301 cggtatctcc agcaaagatt attccgcctt gctgaacgac ccgaatacac cgctggtgaa
     9361 ccgcgccaca cagggggttt atcctcccgc gtctacagtt aaaccctatg tggcggtttc
     9421 ggcattgagc gccggagtga tcacgcgcaa tacgacgttg tttgaccccg gctggtggca
     9481 acttccaggt tcggaaaaac gttatcgtga ctggaaaaaa tggggccacg ggcgtctgaa
     9541 tgtcacaaga tcgctggaag aatctgcgga taccttcttc tatcaggtgg cctacgatat
     9601 ggggatcgat cgcctctccg aatggatggg taaattcggt tatggtcatt acaccggtat
     9661 cgacctggcg gaagaacgtt ccggcaacat gcctacccgc gaatggaaac agaaacgctt
     9721 taaaaaaccg tggtatcagg gtgacaccat tccggttggt atcggtcagg gttactggac
     9781 agcgacccca atccagatga gtaaggcact gatgatcctg attaatgacg gtatcgtgaa
     9841 ggttccgcat ttgctgatga gcaccgccga agacggcaaa caggtgccat gggtacaacc
     9901 gcatgaaccg cccgtcggcg atattcattc cggttactgg gagctggcga aagacggtat
     9961 gtacggtgtt gctaaccgcc ctaacggtac ggcgcataaa tattttgcca gcgcaccgta
    10021 caaaattgcg gcgaaatccg gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta
    10081 taatgcgcac aaaattgccg agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc
    10141 atacaacaat ccgcaagtgg ctgtcgccat gattctggag aacggtggcg cgggtccggc
    10201 ggttggtacg ctgatgcgcc agatcctcga ccacattatg ctgggtgata acaacaccga
    10261 tctgcctgcg gaaaatccag cggttgccgc agcggaggac cattaatcat gacggataat
    10321 ccgaataaaa aaacattctg ggataaagtc catctcgatc ccacaatgct gctgatctta
    10381 ctggcattgc tggtttacag cgccctggtt atctggagcg ccagcggtca ggatattggc
    10441 atgatggagc gtaaaatcgg ccagattgcg atgggtctgg tcatcatggt ggtgatggcg
    10501 caaattcctc cacgcgttta tgaaggctgg gccccctatc tctatatcat ctgtattatt
    10561 ttgctggtgg cggtagatgc tttcggtgcc atctctaaag gcgctcaacg ctggctggac
    10621 ctcggtattg ttcgttttca accgtcggaa attgccaaga tagccgtacc gttgatggtt
    10681 gcacgcttta tcaaccgcga cgtttgccca ccttcattga agaatacagc tatcgcgctg
    10741 gtgctgatat ttatgcccac gctgctggtg gctgcacagc ctgacctggg aacatcaatc
    10801 ctcgttgcgc tttccggcct gtttgtactg ttcctctctg gccttagctg gcgtctgatt
    10861 ggcgtcgcag tagtgctggt agcggcgttc attccgattc tgtggttctt cctgatgcat
    10921 gattaccagc gccagcgcgt aatgatgctc ctggacccgg aatcagaccc actcggcgca
    10981 ggctatcaca ttattcagtc taaaattgct attggctccg gcggattacg cggcaaaggc
    11041 tggctgcacg gcactcagtc acagcttgaa tttctccccg aacgccatac cgactttatc
    11101 ttcgcggtac tggcggaaga gctgggatta gtgggcattt tgattctgct cgctctctac
    11161 attctgctga tcatgcgcgg gctgtggata gccgccagag cgcaaaccac ctttggtcgc
    11221 gtcatggctg gcggcttaat gctgatatta ttcgtttatg tcttcgtaaa tattggtatg
    11281 gtaagcggta ttctgccggt tgtaggggtt ccgctcccac tggtcagtta tggaggatcg
    11341 gcgctaattg tgctgatggc tgggttcggg attgtaatgt caatccacac ccacaggaaa
    11401 atgttgtcga aaagcgtgta agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat
    11461 cgcggcagga atgctcgcgg catgtacaag cgatgatggt cagcaacaga cggtaagtgt
    11521 accgcagcct gcggtatgta acggccccat agttgaaatt agcggggcag acccgcgttt
    11581 cgaaccactg aacgcgacgg caaatcagga ttaccagcgc gacggtaaaa gctacaaaat
    11641 cgtgcaggat ccgtctcgat ttagccaggc gggactggcg gcaatctatg atgccgaacc
    11701 cggcagtaac ctgacggcct ctggcgaagc tttcgatccg acacagttga cggcggccca
    11761 tccaacgctt ccgatcccca gctacgccag aatcactaac ctggctaacg ggcgaatgat
    11821 cgtggtgcgc attaatgatc gcggccctta tggcaacgac cgcgtgattt cgctttctcg
    11881 cgcggcagct gaccgtctta acacgtcaaa caacaccaaa gttcgtatcg atccgattat
    11941 tgtcgcccag gatggttcgc tttctggtcc tggtatggct tgtaccacag tcgccaaaca
    12001 gacttacgcc ctgccagcac ctcccgattt aagcggtggc gcgggaacaa gttcagtgcc
    12061 tgtcccgcag ggtgacattc ttccggtcag taattcgacg ctaaaaagcg aagatccgac
    12121 cggcgcgccg gtaaccagca gcggtttcct cggcgcacca acgaccttag cgcctggtgt
    12181 actggaaggc agcgaaccga cgcctgcgcc acagcccgtt gttacagctc cgtcgacaac
    12241 gcctgcaacc tcacctgcaa tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa
    12301 ctttatggtg caagtcgggg ccgttagcga tcaggctcgt gcgcaacagt accaacagca
    12361 actgggacag aagttcggcg tccccggtcg cgtaactcaa aatggcgcgg tctggcggat
    12421 ccagcttggc ccattcgcca gcaaagccga agccagtacc ttgcagcaac gtttgcaaac
    12481 cgaagcccaa ttacagtcat ttattaccac cgcgcagtag cgtaaagcag gcatctgatg
    12541 tgtcaatttc tgtaaatgac gttaacaaag tcacgcggaa agtcagatgc ctgccggtag
    12601 tgcatttgct atagtagggc acttttttta attccatcac ggatgtcgta gttcagacca
    12661 tgaataccat tttttccgct cgtatcatga agcgcctggc gctcaccacg gctctttgca
    12721 cagcctttat ctctgctgca catgccgatg acctgaatat caaaaccatg atcccgggtg
    12781 taccgcagat cgacgcggag tcctacatcc tgattgacta taactccggc aaagtactcg
    12841 ccgaacagaa cgctgatgtc cgccgcgatc ctgccagcct gaccaaaatg atgaccagtt
    12901 acgttatcgg ccaggcaatg aaagccggta aatttaaaga aactgattta gtcactatcg
    12961 gcaacgacgc atgggccacc ggtaacccgg tgtttaaagg ttcttcgctg atgttcctca
    13021 aaccgggcat gcaggttccg gtttctcagc tgatccgcgg tattaacctg caatcgggta
    13081 acgatgcttg tgtcgccatg gccgattttg ccgctggtag ccaggacgct tttgttggct
    13141 tgatgaacag ctacgttaac gccctgggcc tgaaaaatac ccacttccag acggtacacg
    13201 gtctggatgc tgatggtcag tacagctccg cgcgagatat ggcgctgatc ggccaggcgt
    13261 tgatccgtga cgtaccgaat gaatactcga tctataaaga aaaagaattt acgtttaacg
    13321 gtattcgcca gctgaaccgt aacggcctgt tatgggataa cagcctgaat gtcgacggca
    13381 tcaaaaccgg acacactgac aaagcaggtt acaaccttgt tgcttctgcg actgaaggcc
    13441 agatgcgctt gatctctgcg gtgatgggcg gacgtacttt taaaggccgt gaagccgaaa
    13501 gtaaaaaatt gctaacctgg ggcttccgtt tcttcgaaac cgttaatcct ctgaaagtag
    13561 gtaaagagtt cgcctctgaa ccggtttggt ttggtgattc tgatcgcgct tcgttagggg
    13621 ttgataaaga cgtgtacctg accattccgc gtggtcgcat gaaagatctg aaagctagct
    13681 atgtgctgaa cagcagtgaa ttgcatgcgc cgctgcaaaa gaatcaggtc gtcggaacta
    13741 tcaacttcca gcttgacggc aaaacgatcg aacaacgccc gctggttgtg ctgcaagaaa
    13801 tcccggaagg taacttcttc ggcaaaatca ttgattacat taaattaatg ttccatcact
    13861 ggtttggtta aaaattgaac acttgaaagt gtaatttccg tccccatata ctaagcatca
    13921 gtaaaaaact cccgccttct ggcgggagtt gctatttaat tacgttacgc cggagctgac
    13981 atgaaaacca aacttaacga actgcttgaa ttccctactc cttttactta caaagttatg
    14041 gggcaggcgt tacctgagct ggttgatcag gtggttgaag tggtacagcg ccatgcgcca
    14101 ggtgactaca ccccaacggt aaaaccaagc agcaaaggca actaccactc ggtatctatc
    14161 actatcaacg ccactcatat cgagcaggtt gaaacactgt atgaagaact gggcaaaatc
    14221 gatattgtcc gcatggttct gtaactcgct tctccgttac ccggctcctg gtcgggtaac
    14281 tcccctctcc tcgctgtgat atacttttcc cccactttta ctcattctcc acggagatgc
    14341 cgttttgtat caggataaaa ttcttgtccg ccagctcggt cttcagcctt acgagccaat
    14401 ctcccaggct atgcatgaat tcaccgatac ccgcgatgaa agtacccttg atgaaatctg
    14461 gctggtcgag cactatccgg tattcaccca agggcaggca ggaaaggcgg agcacatttt
    14521 aatgccgggt gatattccgg tgatccagag cgatcgcggt gggcaggtga cttatcacgg
    14581 gccggggcaa caggtgatgt atgtgttgct taacctgaaa cgccgtaaac tcggtgtgcg
    14641 tgaactggtg accttgcttg agcaaacagt ggtgaatacc ctggctgaac tgggtataga
    14701 agcgcatcct cgggctgacg cgcctggtgt ctatgtcggg gaaaagaaaa tttgctcact
    14761 gggtttacgt attcgacgcg gttgttcatt ccacggtctg gcattaaacg tcaatatgga
    14821 tctttcacca tttttacgta ttaatccttg tgggtatgca ggaatggaaa tggctaaaat
    14881 atcacaatgg aaacccgaag cgacgactaa taatattgca ccacgtttac tggaaaatat
    14941 tttagcgcta ctaaacaatc ctgacttcga atatattacc gcttaattcc atacatcaat
    15001 ggcccaattt acactgggtc attactcata atacctgcta atgctttaca tctccttcgc
    15061 gtttgtatat tttctttatc acttgcagaa atgcgatgct acgccttgct ccgtagccca
    15121 cctaattgat taactcattg aaataattga ccatggtgag
//
LOCUS       DACADF010000114        14945 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.116, whole genome shotgun sequence.
ACCESSION   DACADF010000114 DACADF010000000
VERSION     DACADF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.116"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(57..2336)
                     /gene="maeB"
                     /locus_tag="ISB30_003425"
     CDS             complement(57..2336)
                     /gene="maeB"
                     /locus_tag="ISB30_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAP1991026.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2625..3575
                     /gene="tal"
                     /locus_tag="ISB30_003426"
     CDS             2625..3575
                     /gene="tal"
                     /locus_tag="ISB30_003426"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAP1991027.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILNIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3595..5598
                     /gene="tkt"
                     /locus_tag="ISB30_003427"
     CDS             3595..5598
                     /gene="tkt"
                     /locus_tag="ISB30_003427"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP1991028.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVVHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEKYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5675..6718)
                     /locus_tag="ISB30_003428"
     CDS             complement(5675..6718)
                     /locus_tag="ISB30_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAP1991029.1"
                     /translation="MRYRIFLLFFFALLPMSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPQASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6844..7419)
                     /gene="nudK"
                     /locus_tag="ISB30_003429"
     CDS             complement(6844..7419)
                     /gene="nudK"
                     /locus_tag="ISB30_003429"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAP1991030.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7487..9466)
                     /gene="aegA"
                     /locus_tag="ISB30_003430"
     CDS             complement(7487..9466)
                     /gene="aegA"
                     /locus_tag="ISB30_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAP1991031.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9672..11372
                     /gene="narQ"
                     /locus_tag="ISB30_003431"
     CDS             9672..11372
                     /gene="narQ"
                     /locus_tag="ISB30_003431"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HAP1991032.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11536..14649
                     /gene="acrD"
                     /locus_tag="ISB30_003432"
     CDS             11536..14649
                     /gene="acrD"
                     /locus_tag="ISB30_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAP1991033.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14748..14807)
                     /gene="ypfM"
                     /locus_tag="ISB30_003433"
     CDS             complement(14748..14807)
                     /gene="ypfM"
                     /locus_tag="ISB30_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAP1991034.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3488 a         3973 c         3914 g         3570 t
ORIGIN      
        1 tgcagctaaa ccgcttaccc tgaatattca gggtaagcgt gagagttaaa aaagaattac
       61 agcggttggg tttgcgcttc aaccacggcc agcgccacca tgttgacgat acgacgtacc
      121 gatgcgatcg gcgttaacac gtgaaccggc ttcgccaccc ccatcagcac cgggccgaca
      181 gtcacacctt ccgagctgga aacacgcagt aagttgtaac taatgcgggc agcttccatg
      241 ttcggcatca ccagaatatt agcggaacct ttcaaagggc tgtccggcat acggtcgttg
      301 cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac catcaatcat cagttctggc
      361 gcacgttccc tgaccagttc cagcgcctga cgcattttgc tcgacgacgg gcagtcagaa
      421 gaaccaaagt tggagtgcga caacagagcc acacgcggct caataccaaa acgacgaaca
      481 gtttctgccg ccatcaaggt gatctccgcc agctcttctg catcaggctc atcattgacg
      541 taggtatcgg caataaaggt gttaccactc ggcaacagca gcgcgttcat tgcacctgcg
      601 gtgtgaacgc catcgcgata accaaagaca tttttcacca cgctaaagtg ttcatgataa
      661 tcgcccaccg taccgcaaat cattgcatcg gcttcgccac gctgaaccat gatcgcgccg
      721 atcactgtcg ggttactgat cagcgcacgc tgcgcctgct cctgagtgac accgcgacgc
      781 ttcatgatct ggaagtattc ggtccagtac tccttaaagc gcggatcgga ttcgttattg
      841 acgatctcaa aatcaacgcc cgccttgatc tgcaagccca gtttctgaat gcgcatttcg
      901 atcacgttcg gacgaccaat aaggatcggt ttcgccagtc ccagcgttac cagttcctga
      961 gtggcatgta gaacgcgcgc ctcttcccct tctggcagaa ctacgcgctt cggcgctttg
     1021 cgagcctggg agaaaatcgg cttcataaac aggttggttt tgtaaacgaa ctccgtcagc
     1081 ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg ccacgcccga ctccatcgcc
     1141 gctttcgcca ccgcaggagc gatcttaacg atcaagcgcg gatcaaacgg ttttggaatg
     1201 atgtattccg gaccaaagct cagatcctga tcgccatacg ctgaagccac cacttcactc
     1261 tgttccgcat gggccagttc tgcaatcgcg cgtaccgccg ccagtttcat ctcttcgttg
     1321 atggcggttg cgccaacgtc cagcgcgcca cggaagataa acgggaagca aaggacgttg
     1381 ttcacctggt tcgggtagtc ggaacgaccg gtacagatga tggcatccgg acgcacttct
     1441 ttcgccagcg gcggcagaat ttccggttcc gggttcgcca gcgccaggat cattggcgca
     1501 cgggccattt tcttcaccat ttcctgggtc agcactttcg ggccggaaca gccgaggaaa
     1561 atatccgcgc cttcaatcac atcatcgagg gttcgtttgc cgtcatccac caccgcatat
     1621 gcggctttgg tttccgccat gtttggctca cggccctgat agataacgcc ttttgaatcg
     1681 caaaccacga tgttatgttt ttgcagaccc agcgctacca gcaggttcat acaggcgatt
     1741 gctgcggcac ccgcgccaga aaccaccatc cgcacgtcgg agatgttttt ctccaccacg
     1801 cgcaagccgt tgaggatggc ggcagtgctg ataattgccg tgccgtgctg atcgtcgtgg
     1861 aataccggaa tattcatccg ctcgcgcagt ttctgttcaa tatagaaaca ttctggcgct
     1921 ttaatgtctt cgaggttgat gccgccgaag gtcggttcga gcgcagcgac gacttcaata
     1981 aatttgtccg ggtcgagttc atcaacttca atgtcaaata catcaatccc ggcgaatttc
     2041 ttaaacagaa cgcccttgcc ttccatcacc ggtttgcctg ccagcgcgcc aatgttgcct
     2101 aaccccagca ccgccgtacc gttagagatc accgctacca ggttaccgcg cgcggtatat
     2161 ttgtaggctt ttaacgggtc tttttcgatt tcaagacaag gtgcggcaac gcctggtgag
     2221 taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg ttggagaaac ctggattttc
     2281 cctggaactg gaaattcatg gaaatcaagt gcactttgtt ttaactggtc atccatttgg
     2341 ttgttccttt cacgtaacgt tcacaaataa attgtgtggg caacagcccc tgcccacaac
     2401 gtggcgcaca ttattaccct gccggagtct acagactttg agcaagtcca aactctcacc
     2461 attaatataa tgttttggta ataatcatat aacactgatg ttacctgctt aatccagcaa
     2521 taccatgcct gtctgctatg cttttttgat gcgtttagcg aaatttctca gaagtgtgaa
     2581 ttaacgcact catctaacac tttacttttc aaggagtatt tcctatgaac gagttagacg
     2641 gcatcaaaca gttcaccact gtcgtggcag acagcggcga tattgagtcc attcgccatt
     2701 atcatcccca ggatgccacc accaatcctt cgctgttact caaggctgcc ggattatcac
     2761 aatatgagca tttaatagac gatgctatcg cctggggtaa aaaaaatggc aagacccagg
     2821 aacaacaggt ggtcgcagcg tgtgacaaac tggcggtcaa tttcggtgct gaaattctca
     2881 atatcgtacc cggtcgcgtg tcaacagaag ttgatgcacg cctctctttt gataaagaaa
     2941 agagtattga gaaggcgcgc catctggtgg atttgtatca gcaacaaggc gttgagaaat
     3001 cgcgcattct gatcaagctg gcttcgacct gggaaggaat tcgcgcggca gaagagctgg
     3061 aaaaagaagg tattaactgc aacctgacgc tgctgttttc ttttgcacag gcgcgggcct
     3121 gtgcagaagc aggcgttttt ctgatttcgc catttgtcgg gcgtatttat gactggtatc
     3181 aggcacgcaa gccgatggac ccgtatgtgg tggaagaaga tccaggcgtt aaatcagtac
     3241 gcaatatcta cgactactat aagcaacacc actatgaaac cattgtgatg ggcgcgagct
     3301 tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga ccgactgact atcgcaccga
     3361 atttgctgaa ggagctacag gaaaaagttt cgccagtggt acgtaaatta atcccacctt
     3421 ctcagacgtt cccacgccca gctcccatga gcgaagcaga gttccgttgg gagcacaatc
     3481 aggatgcgat ggcggtagaa aaactgtctg aaggcattcg tctgttcgcc gttgatcaac
     3541 gcaaactgga agatcttctt gccgccaaac tataaaccag ccacggagtg ttatatgtcc
     3601 cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg atgcggtaca aaaagccaat
     3661 tctggtcatc ccggcgcgcc gatgggcatg gctgatattg ccgaagtgct gtggaacgat
     3721 tttcttaaac ataaccctac cgacccaacc tggtatgatc gcgaccgctt tattctttcc
     3781 aacggtcacg cgtcgatgct gctctacagt ttgctgcatc tgaccggtta cgacctgcca
     3841 ctggaagaac tgaagaactt ccgtcagttg cattcgaaaa cccctggtca cccggaaatc
     3901 ggttatacgc caggtgttga aaccaccacc ggtcctcttg gacaaggttt ggcgaacgcc
     3961 gtcgggctgg cgatagcaga gcgtacgctg gcggcgcaat ttaatcagcc ggatcatgaa
     4021 attgtcgatc acttcaccta tgtgtttatg ggcgacggct gcctgatgga aggtatctct
     4081 cacgaagtct gttcgctggc gggcacgctg gggctgggca agctgattgg tttttacgat
     4141 cacaacggta tttccatcga cggtgaaacc gaaggctggt ttaccgacga tacggcaaaa
     4201 cgttttgaag cctatcactg gcatgtggtc cacgagatcg acggccacga tccgcaggcg
     4261 gtgaaggaag cgatccttga agcgcaaagc gtgaaagata agccgtcgct gattatctgc
     4321 cgtacggtga ttggctttgg ttcgccgaat aaagcaggta aggaagaggc gcacggcgca
     4381 ccgctggggg aagaagaagt agctctggca cggcaaaaac tgggctggca ccatccgcca
     4441 tttgagatcc ctaaagagat ttatcacgcc tgggatgccc gcgaaaaagg cgaaaaagcg
     4501 cagcagagct ggaatgagaa gtttgccgcc tataaaaagg ctcatccgca actggcagaa
     4561 gagtttaccc gtcggatgag cggcggtttg ccgaaggact gggagaaaac gactcagaaa
     4621 tatatcaatg agttgcaggc gaatccggcg aaaatcgcta cccgtaaggc ttcgcaaaat
     4681 acgcttaacg cttacgggcc gatgctgcct gagttgctcg gcggttcggc ggatctggct
     4741 cccagcaacc tgaccatctg gaaaggttct gtttcgctga aggaagatcc ggcgggcaac
     4801 tacattcact acggggtgcg tgaatttggt atgaccgcta tcgccaacgg catcgcgcac
     4861 cacggcggct ttgtgccgta taccgcgacg ttcctgatgt ttgttgaata cgcccgtaac
     4921 gccgcgcgga tggcggcact gatgaaagcg cggcagatta tggtttatac ccacgactca
     4981 attggtctgg gcgaagatgg tccgacgcac caggctgttg agcaactggc tagtctgcgc
     5041 ttaacgccaa atttcagcac ctggcgaccg tgcgatcagg tggaagcggc ggttggctgg
     5101 aagctggcgg ttgagcgcca caacggaccg acagcactga tcctctccag gcagaatctg
     5161 gcacaggtag aacgaacacc ggatcaggtt aaagagattg ctcgtggcgg ttatgtgctg
     5221 aaagacagcg gcggtaagcc agatattatt ctgattgcca ccggttcaga gatggaaatc
     5281 accctgcaag cggcagagaa attagcggga gaaggtcgca atgttcgcgt agtttccctg
     5341 ccctcgaccg atattttcga tgcccaggat gagaaatatc gggagtcggt actgccttca
     5401 aatgtttctg cccgcgtggc agtagaagcg ggtattgccg attactggta caaatatgtt
     5461 ggcctgaaag gggcaattgt cgggatgaca ggttatgggg aatcagctcc ggcggataag
     5521 ctgttcccgt tctttggctt taccgccgag aatattgtgg caaaagcgca taaggtactg
     5581 ggagtgaaag gtgcctgatg gtgatcgccg gatgctgatt gtcggatgtg acgctggtgc
     5641 gtcttatccg gtctacatgt ccccgccatt ttgtttaacg ggtgatccac aacgtgggcc
     5701 aggcgtctgg cccatgccag ttatcgcagg tgggttctgc ggcgtaacgc accaggcgaa
     5761 aacgctgacc gtcaaagcgc cagcgcgcct gaatgccaca atcgcttaat ccgcgccctt
     5821 ttgctaaggt caccagttca cgcgattttt catcgaatgt tgcgttcatc agttccagtt
     5881 cattggtctc ctgaccgttg ttgaacggca aacgcaaccg taccgggcgc gaggcctgcg
     5941 gctttttacg cgacacaatc catgccaaat caatggtgtt ataggcccct gcctcacagc
     6001 taatcatcat cagcgcttta tcatcggtca gcgcagtgac attcacctca cgacgtaacg
     6061 gatcaagcga acagcgcaga ccattcatcc gccagtttcc ataatccagc aaatcgttac
     6121 gttcttcgag tgagagtggc gtcggcgttg ggttaaccac cgcgacctct ttcagcgcag
     6181 gcgctggcgg tacgctgagc ggcggttcgt cccctttctt tatccacgcg gtctcactgc
     6241 caacgcgctt ttgctgggca tcaataaaca acaacgccgc ttttaagcca ctcagagaaa
     6301 tagtctgatt gccgtcgcgt aaggtgatcg ccctcccttc ctgaatcatc tgcaaaaacg
     6361 cggtgatggt tgccgtatca tcggttacta ataaccatgg tgaaatccgc cacttgtcac
     6421 cacttaacgc caacggctca ccatctaaca gcagccgtgg cgctatctcc ccttctgacg
     6481 catccggcga cttcaacccg ccgcgctcaa tacgtaaaac ggcatcggta tgcgccccgg
     6541 cgctgcggct cagggtcatc accagtccat tatgatcgcc cgtattgcgc gctacacaga
     6601 aattttgatt attacaggtg acctgccagt cggaaaacgc ccgttgcgct ggtgccgccc
     6661 acaccaaaga catcggcaac aaagcgaaaa aaaagagaag gaaaatgcga tatcgcatgg
     6721 acggtacgac cccagaagtt caaacaaaca gcacaagtcg tatcttcgtt gcgcaggtag
     6781 cgcggctcaa tcggatttgt cggattgagc caataataaa caacgaaata ttattgttat
     6841 ttttcagtcc attaaatgtg acatttgcaa atagttaagc aataacaccg tcttaccgtc
     6901 acgtatctcg ccggttttaa tcatctccag cgcctggctg aacggcagct caagcacttc
     6961 aatatcttca tcttcgacac caccaccggc gttagcgcgc tggctgtcac tgtattcggc
     7021 gataaaaaag tggattagct cagtcacacc gcctggcgac atatacagtt caaataattt
     7081 gcggacttcg ccaacttcat agcccgtctc ttcaatcgct tctttgcgaa tgcacacttc
     7141 cggttcgtcg ttatccagca gcccggcgca ggtttcaatc agttgcccgc tttcattgcc
     7201 attaacccag gtagcgacgc ggaactggcg aatcagaacc acggtctttt tcttcgcgtt
     7261 gtacaggagg atcgtcgcac cattaccgcg atcgtaaact tcacgtttat ggcggataac
     7321 ttcgccatct ttgcgcgtga gatcgtaagt aatgttgtgc agggtgaaat agttatcgga
     7381 gagaattttg tctttaacga gggtgatttg ttgcgtcata ccgactccac agcgcgaaat
     7441 gaacaattat cttacgctgt gaagtcgggt ttgtctgcgc aggctatcag tgagatttga
     7501 ctgattttac gcccagccag tcaatgatcc cctgtgccgc atgacgtcct tctgccattg
     7561 cggtaaccac cagatccgca ccacgcacgg cgtcaccacc ggcgaagatt ttcggattcg
     7621 tggtctggta acggtactgg ctttccacat ccgcgatgat gcggccccat ttgtctaccg
     7681 ttacaccgtg cgactccagc cacggcatcc cgtgcggatt gaagccaaac gccataatca
     7741 ccgcatcggc tggcatgaca aattcactgc cttccaccgg cactggacgc cgacgtccct
     7801 gggcatccgg ctcgccaaga cgcgtgcgca ggaaacgaat cccgcagacg tgaccttgtt
     7861 cattcagctc aagcgccacc ggctggacgt taaattcgaa gttggccccc tcttcacggg
     7921 cgttcttcac ctctttcttc gagcctggca tgttagcttc atcacgacga taagcgcagg
     7981 tgacgttact cgcgccatgg cgcagcgcgg tacgcacaca gtccatcgcg gtgtcgccgc
     8041 cgcccagtac cacgacgtta agtccggcgg tattgataaa cggctcttcc ggtagctctt
     8101 cgagtcccat cacctgttta gtgttggcga tgaggaacgg cagcgcgtca taaacgcccg
     8161 gcgcatcttc attgggtaaa cccgctttca tggaacggta agtgcctacg ccaacgaaga
     8221 ccgcgtcgta ttgttccaaa agcgaattca aagagacatc tttacccact tcacaattga
     8281 gttcgaagtg aatccccatc gcgctgaaga tttcccgacg gcgtgccagc agggatttat
     8341 ccagtttgaa agaaggaatg ccgaaagtga gcaagccacc gatttctgga tggcgatcgt
     8401 acaccgtcac cgccacgcca ttgcgggtca gaacatctgc acaggccagc cctgccggac
     8461 ccgcaccgat aatcgccacc cgcttgtcca ctttggtgac atggcttaag tcaggacgcc
     8521 aacctttcgc caacgcctga tctgaaatgt agcgttcaat gttaccgata gttactgcgc
     8581 cgtgctcatc gcgaatagta caggcacctt cacacaaacg gtcttgcggg caaacgcgtc
     8641 cggtaatttc cggcagggtg ttggtctggt gagaaagctc gacggcggcg tcgatgtttc
     8701 cggctttcac cagttcaatc cactgcggta tatggttatg cagcgggcag gtccattcac
     8761 aaacgctgtg ctcgccgcac ttaaggcagc gcgaggcttc ccgttgtgcc tgatcggcac
     8821 ggaatggcag ataaatttca tcaaaaccgg ttttgcgcgc ttcaatcgcc agtttatctg
     8881 gctcgccacg cgcgggcgtt gcctgcattt gctcgacttt actcattacc ggcatttctt
     8941 gcgccgcggt actggcatgc cacggttgat gttcctgacg tgcggtgcgc aagcggcggg
     9001 atttcgccat gccggagagt gcggcgtcag tgaccagttg cagcgcgtcc gccgggcaat
     9061 tttcgacaca ggcaggaccg ttttcgcgcc ctgcacaaag gtcgcattta tgcgccgtgg
     9121 cttttacttt tcctgccgca acgggtgtca gaacgatttg catcgtacca aatggacagg
     9181 ccaccacgca ggatttacag ccaatacact tttgctgatt gacctgaatg ctgtcatcaa
     9241 cgtggctgat tgcgccatta gggcagctac gggcgcaggg cgcatcttca caatggtgac
     9301 aggtcacggc actacgttgc tgctgatgtt tgataaccgt aattcgggga tgaaaatggt
     9361 gttggctcag gacatgttgc tcgtcattgt gagccatgac acaggcgatt tcacaagcat
     9421 gacaacccag acattgctga ctgttggcca taataaaacg attcataacg accttctttt
     9481 ttggttgtaa aaaccttatt ctttatatga gtgttgttat tacccgactt acaggggaat
     9541 cggcaatgtt catgtgccca gaataagtaa ctatttcgct ataaactgtg gcagatcaaa
     9601 taatcccatc actgactaaa ttgcgtttca gcgaactgga agattaatga ttttttgtgg
     9661 agaagacgcg tgtgattgtt aaacgacccg tctcggccag tctggcccgg gccttttttt
     9721 acattgtgct gctgtcgatt ctttccacgg gtatcgctct gctaacgctg gcgagcagtt
     9781 tacgcgacgc tgaggctatc aatattgccg gatcgctgcg tatgcagagt taccgcctgg
     9841 gctacgactt gcaaagtggc agtccacaac tcaatgcaca tcgccagtta tttcagcagg
     9901 cactgcattc accggtatta accaatctca acgtctggta tgtgccagag gcagtaaaaa
     9961 cccgctatgc gcatctgaat gccaactggc tggagatgaa taatcggctc agcaagggcg
    10021 acttgccgtg gtatcaggcc aatattaata attatgttaa tcagatagac ctgttcgtgc
    10081 tggctttaca gcactacgct gaacgcaaaa tgctgctggt ggtggcaatt tccctggctg
    10141 gcggcatcgg tattttcacg ctggtctttt ttactttgcg ccgcatacgc catcaggtgg
    10201 ttgccccgct gaatcagctg gttaccgcca gtcagcgtat tgaacacggg cagttcgact
    10261 cgccgccgct ggataccagc ctgccgaatg agcttggcct gcttgcaaaa acctttaacc
    10321 agatgtcgag cgagctgcat aaattgtacc gttcgctgga ggcgtcagta gaagaaaaga
    10381 cccgcgatct ccacgaggcc aagcgtcgtc tggaggtgtt gtatcagtgt tcgcaggcgc
    10441 taaacaccag ccagattgat gtgcattgtt tccgccatat tttgcagatt gttcgcgaca
    10501 atgaagcggc tgaatatctg gagttaaatg tcggtgacaa ctggcggatt agcgaagggc
    10561 agccaaaccc ggaattgccg atgcagattt taccggtgac aatgcaagag acggtttacg
    10621 gagaactgca ctggcaaaat agtcacgttt catcatcaga accgctgctt aacagcgttt
    10681 cgtcgatgct gggacgcggt ttgtacttta atcaggcgca gaagcatttt cagcagttat
    10741 tgttgatgga agaacgtgcg accatcgccc gcgaattgca cgactcgctg gctcaggtac
    10801 tttcttactt acgtatccag ttgacgttac tgaagcgttc gataccggaa gataacgcca
    10861 ccgcacaaag tatcatggcc gatttttccc aggcgttgaa tgatgcttat cggcagttac
    10921 gcgagctgtt gactactttc cgcctgacgc tgcagcaggc ggatctcccc tccgcgttga
    10981 gggaaatgct ggatacgtta caaaatcaaa ccagcgccaa actgaccctc gactgccgtc
    11041 tgccaaccct ggcgctggat gcgcaaatgc aggtgcattt gttgcaaatt attcgcgaag
    11101 cggtgctgaa tgcgatgaag cacgccaacg ccagcgaaat cgccgttagc tgcgtcaccg
    11161 cgccggatgg caatcacaca gtctatattc gcgacaacgg gattggcatc ggtgaaccga
    11221 aagaacccga aggccattat ggtctgaata tcatgcgcga acgcgcggaa agattgggtg
    11281 ggacgctgac tttttcacaa ccttccggcg gcggcacgtt agtgagtatt agctttcgct
    11341 ctgcggaggg tgaggaaagt cagctgatgt aatgcctctt attgaccaaa gaatagtggc
    11401 acttaaggtt cagtataaaa gggcatgata atttacatta actccttttt ttctccacga
    11461 ttggctcgta ccttgccgct acagtgaagc aagccaagcc tacaacgata cgcagaaaca
    11521 cgaggtcctc ttttaatggc gaatttcttt attgatcgcc ccatttttgc ctgggtgctg
    11581 gcaatcctgt tgtgtctgac aggtaccctg gcgatttttt cattgcccgt tgaacaatac
    11641 cccgatctcg cgccaccgaa tgtgcgagtg accgctaact atcccggcgc atcggcccag
    11701 acgctggaaa acaccgtgac ccaggttatc gagcaaaata tgaccggcct cgataatctg
    11761 atgtatatgt catctcagag cagcggcacc ggtcaggcat ctgtcacgtt aagttttaaa
    11821 gcaggcaccg atccggacga agccgtgcag caagtacaaa accagctgca atcagccatg
    11881 cgaaagttac cgcaggcggt gcaaaatcag ggcgtgacgg tgcgtaaaac cggcgatacc
    11941 aacattctga ccattgcctt cgtctctacc gatggttcga tggataaaca ggatattgcc
    12001 gattatgttg ccagtaatat tcaggacccg ttaagccgcg tgaatggcgt cggggatatc
    12061 gatgcctatg gttcgcaata ttccatgcgt atctggctgg acccggcgaa actcaacagt
    12121 ttccagatga cggctaaaga tgtcaccgat gccattgaat cacagaacgc gcagattgcg
    12181 gttgggcaac ttggtggtac gccttccgtc gataagcagg cgctcaacgc caccattaac
    12241 gcccagtcac tgctgcaaac accagaacag ttccgcgata tcaccttgcg ggtgaatcag
    12301 gacggctcag aggtaaggct gggcgatgtc gccaccgtcg aaatgggggc ggagaaatac
    12361 gattatctta gccgcttcaa tggtaagcca gcctccgggc tgggggtaaa actggcctcc
    12421 ggcgcgaacg aaatggcgac agcggagctg gtgctcaatc gtctcgacga gctggcgcag
    12481 tattttccac acggactgga atacaaggtg gcgtatgaaa ccacctcttt tgtaaaagcc
    12541 tccattgaag acgtggtgaa aacgcttctg gaagctatcg ccctggtttt cctcgttatg
    12601 tatctgttcc tgcaaaactt ccgcgccacg ctgataccca ctatcgccgt gccggtggtg
    12661 ttgatgggaa ccttctcggt gctttatgcc ttcggctata gcgtcaacac cttaaccatg
    12721 ttcgcgatgg tgctggcgat cggtctgctg gtggatgatg ccatcgtggt ggtggaaaac
    12781 gtcgaacgta ttatgagtga ggaaggactc actcctcgcg aagccacacg taaatcgatg
    12841 gggcagatcc agggggcact ggtcgggatt gcgatggtgc tttcggcggt atttgtgcca
    12901 atggccttct tcggcggcac caccggtgcc atctatcgcc agttctctat taccattgtt
    12961 gcggcgatgg tgctgtcagt actggtagcg atgatcctca ctccggctct gtgtgccaca
    13021 ctacttaagc cactgaaaaa aggtgagcat cacgggcaaa aaggcttttt tgcctggttt
    13081 aaccagatgt ttaaccgcaa cgccgaacgc tacgaaaaag gggtggcgaa aattctccac
    13141 cgtagcctgc gctggattgt gatttatgtc ctgctgcttg gcggcatggt gttcctgttc
    13201 ctgcgtttgc cgacgtcgtt cttaccgctg gaagaccgtg gcatgtttac tacctcggta
    13261 cagttgccca gcggttcaac ccagcaacag accctgaaag tcgttgagca aatcgagaaa
    13321 tattacttca cccatgaaaa agacaacatc atgtcggtgt ttgccaccgt gggttctggc
    13381 cctgggggga acgggcaaaa cgtggcgcga atgtttatcc gcctgaaaga ctggagcgaa
    13441 cgcgacagta agaccggcac ctcgtttgcc attatcgagc gtgcaacgaa ggcgtttaac
    13501 aagattaaag aagctcgcgt tatcgccagc agcccgccag caattagcgg tcttggtagt
    13561 tctgcgggtt ttgatatgga gttgcaggac cacgctggag cgggtcacga tgcgctgatg
    13621 gcagcacgaa accagttgct ggcgctggca gcggaaaacc cggagctaac ccgtgtgcgc
    13681 cataacggcc tcgacgacag tccgcagttg cagattgata tcgaccagcg taaagctcag
    13741 gcgctgggcg ttgctatcga cgatattaac gacacactgc aaaccgcctg gggttcgagc
    13801 tatgtgaatg actttatgga tcgcggtcgc gtgaagaaag tctatgtgca ggcagctgcg
    13861 ccgtatcgca tgctgccaga tgacatcaat ctctggtatg tccgaaataa agatggcggc
    13921 atggtgccct tctctgcttt cgcgacctca cgctgggaga caggctcgcc gcgtctggaa
    13981 cgctataacg gttattctgc ggttgagatt gttggggaag ccgcaccggg ggtcagtacc
    14041 ggtacggcga tggatattat ggaatcgtta gtgaagcagt tgccaaacgg ctttggtctg
    14101 gagtggacgg cgatgtcgta tcaggagcgg ctttccggcg cgcaggctcc ggcgctgtac
    14161 gccatttcct tgctggtggt attcctgtgt ctggctgcgc tgtatgaaag ctggtcggtg
    14221 ccgttctcgg taatgctggt cgtgccgctg ggggttatcg gcgcgctgct ggcaacctgg
    14281 atgcgcgggc tggaaaacga tgtttacttc caggtgggcc tgttaacggt cattggttta
    14341 tcggcgaaaa acgccatcct gatcgtcgag tttgctaacg agatgaacca aaaaggccac
    14401 gacctgtttg aagcgacgct ccacgcctgc cgtcagcgtt tacgcccgat tctgatgacc
    14461 tcgctggcat ttatcttcgg cgtattgcca atggcaacca gcacaggtgc cggttccggt
    14521 ggtcagcatg cggtgggtac tggcgtaatg ggcgggatga tttcggccac tattctggct
    14581 atttacttcg tgccgctgtt ctttgtgctg gtgcgccgcc gcttcccgct gaagccgcgc
    14641 ccggaataag caataaaaaa ggcgacatgc caatgtgtcg cccttttcaa ctttccgatt
    14701 aagaacctgc tcagcgggtt cttgctgttt gtactttgtc tcaggaatta cttacgaagc
    14761 ataacttcga taaagtcttt ccagttcccc agttcacgtt caatcataac aacctctctt
    14821 ataattatgg gtattctacg gaaacaatat accgtggtga agctaattta ctcgattgct
    14881 gcgatgacta cctccggggg acaaacctta tgtaaatact atggtcctgc ggtgatgatt
    14941 tgtat
//
LOCUS       DACADF010000115        14869 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.117, whole genome shotgun sequence.
ACCESSION   DACADF010000115 DACADF010000000
VERSION     DACADF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.117"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            46..411
                     /locus_tag="ISB30_003434"
     CDS             46..411
                     /locus_tag="ISB30_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAP1991035.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWRKGADENASTIRLN"
     gene            558..1076
                     /locus_tag="ISB30_003435"
     CDS             558..1076
                     /locus_tag="ISB30_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HAP1991036.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPGLSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAIISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            1066..2292
                     /gene="dgcN"
                     /locus_tag="ISB30_003436"
     CDS             1066..2292
                     /gene="dgcN"
                     /locus_tag="ISB30_003436"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HAP1991037.1"
                     /translation="MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNSETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            2308..2790
                     /locus_tag="ISB30_003437"
     CDS             2308..2790
                     /locus_tag="ISB30_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAP1991038.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLITQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2867..3214)
                     /gene="rplS"
                     /locus_tag="ISB30_003438"
     CDS             complement(2867..3214)
                     /gene="rplS"
                     /locus_tag="ISB30_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP1991039.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(3256..4023)
                     /gene="trmD"
                     /locus_tag="ISB30_003439"
     CDS             complement(3256..4023)
                     /gene="trmD"
                     /locus_tag="ISB30_003439"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP1991040.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTYD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(4054..4602)
                     /gene="rimM"
                     /locus_tag="ISB30_003440"
     CDS             complement(4054..4602)
                     /gene="rimM"
                     /locus_tag="ISB30_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP1991041.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4621..4869)
                     /gene="rpsP"
                     /locus_tag="ISB30_003441"
     CDS             complement(4621..4869)
                     /gene="rpsP"
                     /locus_tag="ISB30_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP1991042.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(5006..6367)
                     /gene="ffh"
                     /locus_tag="ISB30_003442"
     CDS             complement(5006..6367)
                     /gene="ffh"
                     /locus_tag="ISB30_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP1991043.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6459..7325
                     /locus_tag="ISB30_003443"
     CDS             6459..7325
                     /locus_tag="ISB30_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAP1991044.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLMIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIFQQLIS"
     gene            7346..8632
                     /locus_tag="ISB30_003444"
     CDS             7346..8632
                     /locus_tag="ISB30_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP1991045.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8686..9279)
                     /gene="grpE"
                     /locus_tag="ISB30_003445"
     CDS             complement(8686..9279)
                     /gene="grpE"
                     /locus_tag="ISB30_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP1991046.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9402..10280
                     /gene="nadK"
                     /locus_tag="ISB30_003446"
     CDS             9402..10280
                     /gene="nadK"
                     /locus_tag="ISB30_003446"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAP1991047.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            10366..12027
                     /gene="recN"
                     /locus_tag="ISB30_003447"
     CDS             10366..12027
                     /gene="recN"
                     /locus_tag="ISB30_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP1991048.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGYGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            12176..12517
                     /gene="bamE"
                     /locus_tag="ISB30_003448"
     CDS             12176..12517
                     /gene="bamE"
                     /locus_tag="ISB30_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAP1991049.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12579..12869)
                     /locus_tag="ISB30_003449"
     CDS             complement(12579..12869)
                     /locus_tag="ISB30_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAP1991050.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12859..13335)
                     /gene="ratA"
                     /locus_tag="ISB30_003450"
     CDS             complement(12859..13335)
                     /gene="ratA"
                     /locus_tag="ISB30_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAP1991051.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGG
                     WKFTPLSQDACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            13467..13949
                     /gene="smpB"
                     /locus_tag="ISB30_003451"
     CDS             13467..13949
                     /gene="smpB"
                     /locus_tag="ISB30_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP1991052.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            14164..14526
                     /gene="ssrA"
                     /locus_tag="ISB30_003452"
     tmRNA           14164..14526
                     /gene="ssrA"
                     /locus_tag="ISB30_003452"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3798 a         3879 c         3487 g         3705 t
ORIGIN      
        1 ccatctttaa aaacaatccg ctatgctttg aaaaaggaga aagaaatgat gaaaaaattt
       61 atcgccccct tgttggcttt actggttagc ggatgtcaga ttgatcctta tactcacgcg
      121 ccaaccttga ccagcaccga ctggtatgat gttggtatgg aagatgcgat atcgggcagc
      181 gccattaaag atgacgatgc atttagcgat tcacaggcgg atcgcggtct ataccttaaa
      241 ggatatgccg aagggcaaaa gaaaacttgc cagaccgatt ttacttatgc ccgggggctt
      301 tctggtaaaa gctttcctgc cagctgcaat aatgttgaaa atgccagcca actgcatgaa
      361 gtatggcgaa aaggggctga tgaaaatgcc agcacaatac gattaaatta attaccccca
      421 aaataaatta agcatgagat tttactcatt atgttttatt tctcacattg atgacgaccg
      481 catgaaagga tgatatttta atgagcattc tgaaaaaata tcgatgcatt tcgagcgaag
      541 atggtgagga tccctgaatg cgtttttctc accgactgtt cttactgctg attctcctgc
      601 tgacgggagc accaatactc gctcaggaac cttctgacgt tgcgaaaaac gtgcgtatga
      661 tggtttccgg tattgtcagt tacacccgct ggcctgggct atcagggcca ccaaagttat
      721 gtatcttttc atcttcacgt tttagtaccg ccttacaaga aaatgctgct acatctttac
      781 cttatctgcc tgtcatcatt catactcaac aagaggcgat aatttcaggc tgcaatggtt
      841 tttattttgg caacgagtca ccgacattcc agatggaatt aaccgaacaa tatccgtcaa
      901 aggcgttatt attaattgcc gaacaaaata ccgagtgcat tattggtagc gccttttgtc
      961 tgatcatcca caataatgac gtcagatttg ccgtaaacct ggatgcctta tcgcgtagcg
     1021 gtgtaaaggt caacccggat gtcttaatgc tcgcacggaa gaaaaatgat ggataacgat
     1081 aattctcttc ataagcgccc cacgtttaaa agagcattac gcaacatcag catgaccagc
     1141 atatttatca ctatgacgct gatctggctg ctgctttccg tgacgtcagt gctaaccctg
     1201 aaacaatacg cgcaaaaaaa cctggcactg acagcagcaa caatgactta cagtctggaa
     1261 gcagctgtcg tttttgccga tggccctgca gcaactgaaa cactggcagc gctgggccag
     1321 caaggccaat tttcaactgc agaagtgcgt gataagcagc aaaatattct ggcatcctgg
     1381 cattacaccc ataaggaacc aggcgatact ttcagcaatt tcataagcca ctggctcttc
     1441 cctgccccca tcattcagcc gattcgtcac aatagtgaaa ccattggcga agtacgctta
     1501 actgctcgcg acagttcaat cagccatttt atctggtttt cgctcgctgt cctgaccggt
     1561 tgtattctgc tggcatcagg catcgcaatt accctcaccc gccatttgca caatggcctg
     1621 gtggaagcgc tgaaaaatat caccgatgtc gtacatgatg tgcgttctaa ccgcaatttt
     1681 tcccgacgag tttcggaaga acgtatcgct gagtttcacc gcttcgctct cgacttcaac
     1741 agtctgctgg atgaaatgga agagtggcag cttcgtttac aggccaaaaa tgcgcagctt
     1801 ctacgtaccg cgctacatga cccattaacc gggctggcta accgcgcagc gtttcgtagc
     1861 ggcatcaaca cgttgatgaa caattccgat gcccgaaaaa cgtcggcgtt actatttctt
     1921 gatggcgata atttcaaata tatcaatgat acctggggtc atgcgacggg cgatagagtc
     1981 ttgattgaaa tagcaaaacg gttagctgaa tttggcgggc tgcgacataa agcataccgc
     2041 ctgggcggcg atgaattcgc tatggtgctc tatgatgtac agtcggaatc tgaagtgcag
     2101 cagatatgct cagcactgac acaaattttt aatctcccgt ttgatcttca taatggccat
     2161 caaactacca tgacattaag cattggttac gcgatgacca ttgagcacgc ctctgcggaa
     2221 aatttacaag agcttgccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaaa
     2281 ctggtgagat aacaaggata tatcgatatg ataaagcacc tgctagcccc cctgattttc
     2341 acctcgctaa tacttactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc
     2401 gccgctatgc aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca
     2461 gatgccattc tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacaaatc
     2521 caaaccatgg cagccaaatt ggcctcgaca gggctaacac atgcccgtat ggatggacac
     2581 accgacaact atggtgaaga cagttacaac gaagtcttat cattaaaacg ggcgaatgtc
     2641 gtggccgatg catgggctat gggtggacaa attccgcgca gcaatctcat cacacagggt
     2701 ttaggaaaaa aatatcccat agccagtaac aagaccgccc agggccgcgc cgaaaaccgc
     2761 cgcgtcgcag tggtgattac aaccccttaa aacacgagca aacagacaaa aaaagggcca
     2821 gccaattggc cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct
     2881 ctttgatacg agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa
     2941 cagcaccacg acgtttgaca gaaatgctgt caactaccgg agagtgagtc tggaagacac
     3001 gctcaacgcc ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt
     3061 tacgaattgc gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa
     3121 cccatacttt cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct
     3181 gctcttgttc aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac
     3241 tgatatctcg ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt
     3301 tttgaactcc gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc
     3361 aggtcttcta agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc
     3421 atggttgccc gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt
     3481 atagtgcggg caatccagca atccttcagc aaaggagtct tccgttgccg aggcctcatg
     3541 tcccagtact cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc
     3601 accaccactg agaacgtaat cgccgattga ccattcttca tcaatttcgg tttggatcac
     3661 gcgctcatct ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccag
     3721 ctcgctgacg cccgcttgat caagcttgcg tccctgtggt gacagataaa tcacctttgc
     3781 gccttcaccc gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat
     3841 taacatcccc ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc gatcatacgt
     3901 gaagtcgcga ggactccagc tctggatgct cagcaggcca tttttaactg cccggccagt
     3961 taccccgtaa tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca
     4021 catagcgccg tcttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc
     4081 gattgaacga gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag
     4141 acgttccttg ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattaga
     4201 tccggtttcc atcatatcta cgactttacc gagatcgtag ccttcagtgg ttactacctg
     4261 gcagcccatc aggtctttcc agtagtagtc accctcttca agctgaggca actgcgatga
     4321 atccacgaca atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt
     4381 cagcttgatg atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca
     4441 ctgacccgcc ttctggataa accagggctg atagtcaaaa atgctttcgg cgtcttcggt
     4501 ggaagaaaac actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac
     4561 gatgggatca acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga
     4621 ttaagctgct ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc
     4681 ctggccaacc cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct
     4741 agcgattggg ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct
     4801 gtcagcgaca acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat
     4861 agttaccata acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc
     4921 cggtgtcata ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc aatcagcagt
     4981 taataactct gtcgcataag gtcatttagc gaccaggaaa tcccggcggc atcatccctt
     5041 tcatgcttct catcatcttc gccatcccgc ccttcttcat tttcttcatc atgcgctgca
     5101 tgtcgtcgaa ctgtttcaga agacggttaa cgtcctgcac ctgcataccg caaccggcag
     5161 cgatacggcg cttacgcgaa cctttgatga tttctggctt agcgcgctct ttcatcgtca
     5221 tcgagttgat gatggcttcc atacgcacca gcactttatc gtccatctgc gacttgacgt
     5281 tatccgggat ctgccccatg cccggcagct tgcccatcag gctagccatg ccgcccatat
     5341 ttttcatctg gcgcagctgc tcaagaaagt cgttgagatc gaagccgtcg ccttttttca
     5401 gcttgctggc taatttctca gcttgcgcgc ggtcaacttt gctttcgata tcttcgatca
     5461 gtgacagtac gtcgcccatg ccgagaatac gggaggcaat acggtccgga tggaatggct
     5521 ccagcgcctc ggttttctcg ccaacaccga ggaacttgat cggtttgcca gtgatgtgac
     5581 gaatagagag cgccgcaccg ccgcgggcat cgccgtccac tttggtcaac actacaccgg
     5641 taagcggtaa cgcttcattg aatgcttttg ccgtattggc cgcatcctga ccggtcatgg
     5701 catcgaccac aaatagggtt tcaaccggat taatcgacgc atggacttgt ttgatctcgt
     5761 ccatcatcgc ttcgtcaacg tgcagacgac cggcagtatc caccagcagc acgtcgtaga
     5821 atttcagttt ggcttctttc agcgccgcgt taacgatatc taccggcttc tgaccaacat
     5881 cagaagggaa gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt ttgattgccg
     5941 ccgggcgata gacgtcggca gaaaccacca gtactttctt cttgtgcttc tcgcgcagga
     6001 acttaccgag tttaccaacg ctggttgttt taccggcacc ttgcaggccc gccatcagca
     6061 cgaccgcagg cggttgcgca gccaggttca gggtctggtt ctcttcgccc atcgccgcaa
     6121 ccagttcgtt acggacaatt ttgacgaact cctgccccgg cgtcaggctc ttattaactt
     6181 catgaccaac cgctttctct tttacgcgat tgataaactc acgcactacc ggcagagcga
     6241 cgtcagcctc cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag
     6301 tgaggcgtcc acggccactg atattgcgca gcgtgcgcga caaacgatcg gttaaattat
     6361 caaacattgt ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca catcatcagt
     6421 attttgccgc agtataacat gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt
     6481 cacctgacgc tatactgctt ctctttctta ttgctcaaac tgtcgacatt actatgcccg
     6541 tttttgctct gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc
     6601 tgctgcaaaa aaacggcggc tggcggcgca tggctattat ttctgcggtc atcgcgctgg
     6661 tctgccacgc aatcgctctg gaagcccgca tcctgcccga cggtgatagc ggacaaaacc
     6721 tcagcctgct gaacgttggt tcattggtca gtttgatgat ctgtacggta atgaccattg
     6781 tggcttctcg caatcgtggc tggttgttgc tacccattgt ctatgccttt gcgcttatca
     6841 acctagcgct ggcgaccttt atgcccaatg aatacatcac ccatctggaa gcaacgccgg
     6901 ggatgctggt gcacattggc ttatcgcttt tttcctatgc cacgctaatt atcgctgccc
     6961 tgtacgcgct gcaactggcg tggattgatt accaactgaa gaacaagaag ctggcgttta
     7021 accaggaaat gccgccattg atgagtatcg agcgtaaaat gttccatatc acgcagattg
     7081 gcgtggtgct gctaacgctc actctttgca ctggcctgtt ctacatgcac aacttattta
     7141 gcatggaaaa tatcgacaag gccgtgctct ctatcgtggc gtggtttgtc tatattgtgc
     7201 tgctgtgggg acattatcat gaaggatggc gtggacgccg cgtcgtctgg tttaacgttg
     7261 cgggtgcagt cattctgaca ctggcctact tcggcagccg aatttttcag cagttaatca
     7321 gctaaaccca gaaaaggagt ttcccctgga acacatttct actactacgt tgatcattat
     7381 tctgatcatc atggtggtca tttcagccta tttttccggg tccgaaaccg gaatgatgac
     7441 cctcaaccgc tatcgtctgc ggcatatggc gaaacagggt aatcgctcgg ccaaacgcgt
     7501 cgaaaaattg ctgcgtaagc cagaccgcct gataagcctg gtgttaatcg gcaataacct
     7561 ggtcaatatt cttgcctccg cgctcggcac tattgttgga atgcgtttgt acggcgatgc
     7621 gggcgtggca attgcgactg gtgtgctgac ttttgtcgtg ctggtttttg ctgaggtatt
     7681 gccaaaaacc attgccgcgc tgtacccgga aaaagtcgct tatccgagta gttttctgct
     7741 ggctccgctg caaattttga tgatgccgct ggtctggttg ctgaatgcta ttacccgcat
     7801 gttaatgcgc atgatgggca tcaaaaccga tatcgtggtt agtggctctt taagcaaaga
     7861 agaattacgc accattgtgc acgaatcgcg gtcacaaatt tcccgtcgca atcaggatat
     7921 gctgctgtcg gtactcgatc tggaaaagat gaccgtcgat gacatcatgg tgccgcgcag
     7981 tgaaattatc ggtattgata tcaacgatga ctggaaatcg attctgcgcc aactctccca
     8041 ctcacctcac gggcgcatcg tgctctaccg tgattcgctg gacgacgcca tcagtatgct
     8101 gcgtgtgcgt gaagcctggc gactcatgtc ggagaaaaaa gagttcacca aagaaaccat
     8161 gctacgcgcc gcggacgaga tctattttgt cccggagggt acgccgctca gcacacagtt
     8221 ggtgaagttt cagcgcaaca aaaagaaagt cggcctggtc gtcaacgagt atggagacat
     8281 tcaggggctg gtgacggtcg aagatattct cgaagagatt gtcggcgatt tcaccacgtc
     8341 gatgtcgcca acacttgccg aagaggtcac gccgcaaaac gacgggtcgg tgattatcga
     8401 tggcaccgcc aacgtgcgag aaattaacaa agcctttaac tggcatctac cggaagatga
     8461 tgctcgtact gttaacggcg tcattctgga agcgctggag gagattccgg tagcaggcac
     8521 ccgcgtgcgt ataggcgagt acgatatcga tattctcgac gtacaggaca atatgattaa
     8581 gcaggtaaaa gtttttcctg taaaaccgct gcgcgagagt gtggcggagt aaaaaaacgg
     8641 cccggcattc gcatgctgga ccgtgattta ttacgaaaac agaaattaag cttttgcctt
     8701 cgctacagtc accatcgccg cacgaattgt acgaccgttc agcgtataac ccttctgcat
     8761 aatgcccagt acgttacctg gcgcaacgtc atcagattcc accattgcga tggcctgatg
     8821 cacattcggg tccagtggta cgttagtttc ggcgatcact tcaacgccaa acttacgcac
     8881 aacatccagc atcgacttca gcgtcagctc aataccttca accatcgcgg acatatccgg
     8941 gttagcttta tcagccactt ccagcgcacg atccaggcta tcaatcaccg gcagcaattc
     9001 gttgatgaat ttctccagcg cgaatttgtg agctttttca atatccagtt cagtacgacg
     9061 acgcaggttt tccatttcgg cttttacgcg caaaatgccg tcacgttcac gggtctgggc
     9121 ttcagccagc tgagcttcga gattcgcaat tttttcatcg cgcggatcca cctgctcagc
     9181 agaagcttct ggctcaactg cctcaatctc ttcgtgctga tccatgataa tttcttccgg
     9241 ggcttgcccc tcaggcgttt tctgttcttt actactcatg aatttctccg cgtttttttc
     9301 gcattcatct cgctaacttc gcttattatg gggatcagtt tcagggtttc aagggaagca
     9361 ctcacattgt catcaatctt cgcaacaagg acctcggaaa aatgaataat catttcaagt
     9421 gtattggcat tgtgggacac ccacggcacc ccactgcact gacaacacat gaaatgctct
     9481 accgctggct gtgcacaaaa ggttacgagg tcatcgttga gcaacaaatc gctcacgaac
     9541 tgcaactgaa gaatgtgaaa actggcacgc tcgcggagat tgggcaacag gctgatctcg
     9601 cggttgtcgt tggcggcgac ggtaatatgc tgggcgcggc gcgcacgctc gcccgctacg
     9661 atattaaagt tattggaatc aaccgtggca acctgggttt cctgactgac cttgatcccg
     9721 ataacgccca gcaacaatta gccgatgtgc tggaaggcca ctacatcagc gagaaacgtt
     9781 ttttgctgga agcgcaagtc tgtcagcaag attgccagaa acgcatcagc acggcgatta
     9841 acgaagtggt acttcatcct ggcaaagtag cgcatatgat tgagttcgaa gtgtatatcg
     9901 acgagatctt tgcgttttcg cagcgttccg atggactgat tatttcgacg ccaacaggct
     9961 ccaccgcata ttccctctct gcgggcggtc ctattctgac accctctctg gatgcgatta
    10021 ccctggtgcc catgttcccg catacgttgt cagcacgacc actggtcata aacagcagca
    10081 gcacgatccg tctgcgtttt tcgcatcgcc gtaacgacct ggaaatcagt tgcgacagcc
    10141 agatagcact tccgattcag gaaggtgaag atgtcctgat tcgtcgctgt gattaccatc
    10201 tgaatctgat tcatccgaaa gattacagtt atttcaatac attaagcacc aagctcggct
    10261 ggtcaaaaaa attattctaa ttttacgcca gcctctttac tgtatataaa accagtttat
    10321 actgtacaca ataacagtaa tggtttttca tacaggaaaa caactatgtt ggcacaactg
    10381 accatcagca actttgctat cgttcgtgag cttgagattg attttcatag cggcatgacc
    10441 gtaataactg gcgagaccgg cgcgggtaaa tctattgcaa tagatgccct cggcctttgt
    10501 ctcggtggtc gcgctgaagc cgacatggtg cgtaccggcg ctgctcgtgc cgacctgtgc
    10561 gcccgttttt ctctgaaaga tacgccagcg gccctgcgtt ggctggaaga aaaccagctt
    10621 gaagacgggc atgaatgttt gcttcgtcgc gtaatcagca gcgatggtcg ctcccgtggt
    10681 ttcatcaacg gtacagctgt tcctctgtca caactgcgcg aactgggtca gttgctgatt
    10741 cagatccatg gtcagcacgc tcatcagtta ctcaccaaac ccgagcacca aaaattcctg
    10801 cttgatggct atgccaatga aacctctcag ctccaggaaa tgaccgcacg ttatcagttg
    10861 tggcatcaaa gctgccgtga cctcgcgcat catcagcagt taagtcagga acgcgccgcc
    10921 cgtgcggagc tgctgcaata ccaattaaaa gaacttaacg aatttaatcc gcagcctgga
    10981 gagtttgagc aaatcgacga agagtacaaa cgtctggcga acagcggtca attgctgacc
    11041 accagccaga atgcattggc attaatggct gacggtgaag acgcaaatct gcaaagtcaa
    11101 ctttacacgg ctaaacaact ggtgagcgaa ttgattggca tggacagcaa actgtccggc
    11161 gtacttgata tgctggaaga ggctaccatc cagattgctg aagccagtga tgaactacgc
    11221 cactactgcg atcgtctgga tctcgatccc aaccgactgt ttgaacttga acagcgcatc
    11281 tcaaaacaga tttcgctggc acgtaaacat cacgtcagcc ctgaggcatt gccacagtat
    11341 taccagtcgc tactggaaga acagcagcaa ctggacgatc aggccgactc acaagaaacg
    11401 ctcgcgctgg cggtaacgaa acatcatcag caggcactgg aaacggcgcg cgcgttacac
    11461 cagcaacgcc agcattatgc aaatgagttg gcacagctga tcaccgatag tatgcatgca
    11521 ctctctatgc cacatgggca gtttacgatc gatgttaaat ttgacgagca tcacctgggc
    11581 gctgacggcg ctgatcgtat tgagtttcgg gtaaccacca acccaggtca gccaatgcag
    11641 cctattgcca aagtcgcatc cggtggtgag ttgtcccgca tcgcattggc aatccaggtc
    11701 attaccgcgc gtaaaatgga aaccccagca ctgatttttg atgaagtgga tgtagggatt
    11761 agcggcccaa cagcggcagt tgtcggcaaa ctgctacgtc aacttggcga atcaactcag
    11821 gtgatgtgtg ttacccacct gccacaagtc gcgggatatg gtcatcaaca ctattttgtc
    11881 agcaaagaaa ccgatggtgc gatgacagaa acgcatatgc aatccctgga taaaaaagcg
    11941 cggttacaag agttggcgcg cctgcttggt ggcagtgaag tcacacgtaa cacactggcg
    12001 aatgcgaaag aactgcttgc ggcgtaaact tttttcctgc ttcacggtca gagtaaacag
    12061 caaaacgccg taagaccgga aagcaaaagg ttttaaagtg atgaaaggtc tattatcatc
    12121 ggcatattac agatgagcca cgtactgctc gggcccgaaa aggaatcaaa tcactatgcg
    12181 ctgtaaaacg ctgactgctg cagcagcagt actattgatg ttgaccgcag gctgttccac
    12241 tctggagcga gtggtttacc gtcctgacat caaccagggg aactatctga ccgctaacga
    12301 cgtatccaaa atacgtgttg gcatgacgca acaacaagtt gcgtacgcat tgggtacacc
    12361 gctgatgtcc gatccatttg gtacaaatac ctggttctat gtcttccgcc agcaacctgg
    12421 tcatgaaggt gtaacgcagc aaacgctgac gctgaccttt aacagtagcg gtgtgttgac
    12481 caacattgat aacaaacctg cgctgagtgg taactaataa taaagttgct ctcaaagacg
    12541 ttaaaaaagg tgctcaatga gcaccttttt tctgtctgtt atttattcgc tgatttttct
    12601 gctcgttgcc tgcgaagctc tttcggatcg gcaatgagag gacgataaat ctccacccga
    12661 tcgccatcat gcacaatatc gctgagtttt gccggacggc tgtaaatgcc gactttgttt
    12721 ttagttaaat cgatatcggt acgcaattcc agcaaaccac tggcgcgaat agcttcttca
    12781 accgtcgcgc cctcctgcaa cgtcacgcgc tgcaggtact gcttctcagg tagcgcataa
    12841 gccacctcaa cggcaatttt acctggcact gtagacctct tttgcacgaa cagtaaaagc
    12901 ctgcaccata ttagctgcca gctctttaaa cacacgacca aaggcgagtt caatcaactt
    12961 attggtaaac tcaaagtcga gatgaaactc aatacggcac gcgtcctggc tcaacggcgt
    13021 aaacttccaa ccgcctatca atttcttaaa cggtccatca accagactca tgagaatact
    13081 ttgattactg gtcaactggt tgcgggttgt aaacgttttg ctgatcccag ccttagacac
    13141 atctaccgca gcggtcatct gcccaggagt ggactccaga atccggcttc cagtgcaccc
    13201 tggtaaaaac tgaggataag actgaacgtc attcactaac tgatacattt gctccgcgct
    13261 gtagggtacc agtgcggtcc ggctaatctg aggcataaca atttccatca acaaaaatcc
    13321 aacaaataat atcatttatc ctgctgtaaa aaaaacgcta tcccggcgct gggtaacatc
    13381 gggatcatgc taagatagag ccttgtcccc cgcaggattg atatggggtg ttttcgattt
    13441 cagattaccg atgattcacg acgcttatga cgaagaaaaa agcacataaa cctggttcag
    13501 cgaccatcgc gcttaacaag cgcgcccgtc acgaatactt tatcgaagaa gagttcgaag
    13561 cgggacttgc cctgcaaggc tgggaggtta aatccctgcg tgcaggaaaa gccaatatca
    13621 gcgacagcta cgtccttctg cgtgacggag aggcatttct gtttggcgct aacatcacgc
    13681 caatggctgt ggcctccacg catgtggtgt gcgatcctac ccgtacccgc aagttacttc
    13741 tcaaccagcg cgaactggac tcattgtacg gtcgcgtaaa tcgagaaggc tataccgtag
    13801 tggcgctctc cctgtactgg aaaaatgcct ggtgcaaagt gaaaatcggc gtcgccaaag
    13861 gtaaaaaaca gcacgataaa cgttcagata tcaaagaacg cgaatggcag gtggataaag
    13921 cgcgtatcat gaaaaacgcc catcgttaaa cctatataag cgatactaat caaatcctca
    13981 ccgcgcctcc ctatccggtg gcgcgaatga acatcttgtc gactgtcaaa tctgacacag
    14041 atgtagccat cccattgctt aatcgaaaaa aaatcatact atgcaggcgc taattccagg
    14101 atgataacgc cttttgggct agtcatgctg ctcataaatc tggtatactt accgttacac
    14161 attggggctg attctggatt cgacgggatt tgcgaaaccc aaggtgcatg ccgaggggcg
    14221 gttggcctcg taaaaagccg caaaaaatag tcgcaaacga cgaaaactac gctttagcag
    14281 cttaataacc tgcttagagc cctctctccc tagcctccgc tcttaggacg gggatcaaga
    14341 gaggtcaaac ccaaaagaga tcgcgtggaa gccctgcctg gggttgaagc gttaaaactt
    14401 aatcaggcta gtttgttagt ggcgtgtccg tccgcagctg gcaagcgaat gtaaagactg
    14461 actaagcatg tagtaccgag gatgtaggaa tttcggacgc gggttcaact cccgccagct
    14521 ccaccaaata atgattggac ggtgtaagga ctacaccaac aaaaacagga agttataagt
    14581 ctcagcagga caccgaccag acggtgagga gacaaaaaag gatacgcaac ggagtcgcga
    14641 ctccctatga cattaaagcc cgctgatgcg ggctttttta ttggtcctga cccgccctga
    14701 atagaattca tccaattaca gtccagacat atacagtatc cctcagctca gtatcagctt
    14761 ctcgacaaat ggtcactgtt atttacccgt ttccagaaag agaataattt gcccccatag
    14821 ccagccttcc cggaatctgt atgttcatta atattgttat cgatgccat
//
LOCUS       DACADF010000116        14497 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.118, whole genome shotgun sequence.
ACCESSION   DACADF010000116 DACADF010000000
VERSION     DACADF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.118"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(223..966)
                     /gene="traF"
                     /locus_tag="ISB30_003453"
     CDS             complement(223..966)
                     /gene="traF"
                     /locus_tag="ISB30_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HAP1991053.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKISPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEYYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPKF"
     gene            complement(959..1216)
                     /gene="trbE"
                     /locus_tag="ISB30_003454"
     CDS             complement(959..1216)
                     /gene="trbE"
                     /locus_tag="ISB30_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HAP1991054.1"
                     /translation="MKVIFTSNRLVDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTGLQKADERKNDERNEDE"
     gene            complement(1243..3093)
                     /gene="traN"
                     /locus_tag="ISB30_003455"
     CDS             complement(1243..3093)
                     /gene="traN"
                     /locus_tag="ISB30_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HAP1991055.1"
                     /translation="MKRILPLILALVTGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCSRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPVAGTVESASLSVHAGLYFL
                     NSRYDFMNTTFNVQLVTGQSGTFTIPVPSGFSVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLKGTECSEPGGTKT
                     GVMEGKPWSITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESKTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(3090..3728)
                     /gene="trbC"
                     /locus_tag="ISB30_003456"
     CDS             complement(3090..3728)
                     /gene="trbC"
                     /locus_tag="ISB30_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HAP1991056.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTTENRQFLKQQETLSQQL
                     REKPDIQLQAWAEQQVRANPLQHSDNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(3737..4042)
                     /locus_tag="ISB30_003457"
     CDS             complement(3737..4042)
                     /locus_tag="ISB30_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991057.1"
                     /translation="MATMDSKYIQHLRKIGAVLLRLISRTLNLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCVFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(4072..5064)
                     /gene="traU"
                     /locus_tag="ISB30_003458"
     CDS             complement(4072..5064)
                     /gene="traU"
                     /locus_tag="ISB30_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HAP1991058.1"
                     /translation="MKRRLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(5061..5693)
                     /gene="traW"
                     /locus_tag="ISB30_003459"
     CDS             complement(5061..5693)
                     /gene="traW"
                     /locus_tag="ISB30_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HAP1991059.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRIYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(5690..6076)
                     /gene="trbI"
                     /locus_tag="ISB30_003460"
     CDS             complement(5690..6076)
                     /gene="trbI"
                     /locus_tag="ISB30_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HAP1991060.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(6073..8700)
                     /gene="traC"
                     /locus_tag="ISB30_003461"
     CDS             complement(6073..8700)
                     /gene="traC"
                     /locus_tag="ISB30_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HAP1991061.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGVPFCIHLKS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLPEGMNLPLTLRHYRV
                     FFSYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNYKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAARDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(8860..9081)
                     /gene="traR"
                     /locus_tag="ISB30_003462"
     CDS             complement(8860..9081)
                     /gene="traR"
                     /locus_tag="ISB30_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HAP1991062.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQEKQRKHYA"
     gene            complement(9216..9731)
                     /gene="traV"
                     /locus_tag="ISB30_003463"
     CDS             complement(9216..9731)
                     /gene="traV"
                     /locus_tag="ISB30_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HAP1991063.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKELEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATPPSGSRPAVTALPEQKLLAPR
                     PLFTAAREVKTIVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(9731..10090)
                     /locus_tag="ISB30_003464"
     CDS             complement(9731..10090)
                     /locus_tag="ISB30_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HAP1991064.1"
                     /translation="MFISLMTGQTETTEIKMNMRNIITARSVPDKSVSDDFMNAVLSN
                     CTTRIVLPALKEFGSESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGQPCAGKTNL
                     TVELISAVVESNKKGEG"
     gene            complement(10029..10616)
                     /gene="traP"
                     /locus_tag="ISB30_003465"
     CDS             complement(10029..10616)
                     /gene="traP"
                     /locus_tag="ISB30_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HAP1991065.1"
                     /translation="MPARRAEMAGDALTFRVAFVVRWLLWVVRFVVIWPLATMALMAL
                     FVLWKDNTTLGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAAD
                     AADYVRETDRSLMQLVTAFWATLALMYVSLAAITGKYPVRPGKMKCVRVGTADEHLKE
                     VYTEDTSLSEKIRKCHVYFPDDRANRNNGDKNEHA"
     gene            complement(10585..12036)
                     /locus_tag="ISB30_003466"
     CDS             complement(10585..12036)
                     /locus_tag="ISB30_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HAP1991066.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVMYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKNGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGDKNPQGGDAGKEGGNGG"
     gene            complement(12036..12740)
                     /gene="traK"
                     /locus_tag="ISB30_003467"
     CDS             complement(12036..12740)
                     /gene="traK"
                     /locus_tag="ISB30_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HAP1991067.1"
                     /translation="MFGSLFFSCSVMAANGTLSPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGACMDVYVIRDGEGN"
     gene            complement(12751..13317)
                     /gene="traE"
                     /locus_tag="ISB30_003468"
     CDS             complement(12751..13317)
                     /gene="traE"
                     /locus_tag="ISB30_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HAP1991068.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLKYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(13339..13650)
                     /gene="traL"
                     /locus_tag="ISB30_003469"
     CDS             complement(13339..13650)
                     /gene="traL"
                     /locus_tag="ISB30_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HAP1991069.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWV
                     K"
     gene            complement(13655..14017)
                     /gene="traA"
                     /locus_tag="ISB30_003470"
     CDS             complement(13655..14017)
                     /gene="traA"
                     /locus_tag="ISB30_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HAP1991070.1"
                     /translation="MDAFLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAQGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGLK"
     gene            complement(14051..14278)
                     /gene="traY"
                     /locus_tag="ISB30_003471"
     CDS             complement(14051..14278)
                     /gene="traY"
                     /locus_tag="ISB30_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HAP1991071.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVIEENESTFKEL"
BASE COUNT         3350 a         4088 c         3458 g         3601 t
ORIGIN      
        1 aatctgctcc atgcgggaga ggaaaatctc ttttcgggtc tgacggcgct tagtgctgaa
       61 ttcactatcg gcgaaggtga gttgatggct catgatgtcc ctctgggatg cgctccggat
      121 gaatatgatg atctcatatc aggaacttgt tcgcaccttc cttaacagaa caacacctgt
      181 aaacattttc aaataatctt cactcataaa tcaaatccgc gattaaaatt ttggtttaaa
      241 atcttcagaa acgttcagga attgttttgc caggtcgtcc tgcgaaataa agccgtatga
      301 taacgggcga acactgccct gtttcgggtc aaccagcatc atggccggga aatatttcac
      361 gccgaggcgc tgcgcctgcc cctggtcagt ccgggaatcc ggcaacagcg gattaatcac
      421 gccatccacg gaaacgggaa taacactcag accatacgta tcccggaagc cattaatgac
      481 ctgcgccagc tgcccgtcga tggggtcctg cccccggtaa aaaaacatga tgccgtagta
      541 ttcagccagt ttcgcaatgg cctgtcgctg ctgcgcctgg tctgccgcca gctggttccg
      601 taccgtgccg ttgtaatggc tgtactgcag gttatagtcc agttcgggat gtgccagcat
      661 ggccttttta gcactcatgg tgaaaagccc ggcctgctga gtccagtaat tctgcagccg
      721 gaaatatttc acaaaattat ccacgccggg atacagaatg gcttcgtaca gcgcccgctt
      781 tgttgccgtc tgcagcgcgg ccagtttctg cataatatcc ggctcctgac ggggggctgc
      841 aggtacaggt ttattttctt tttcctttgg acttattttc tcgttatacc actgccagcc
      901 tgcatctttc cctgacgccg gaaaaataaa gcagcagagt aacagtggca gtaatgcttt
      961 attcatcttc attcctttca tcattttttc tttcatcagc tttctgcagg ccagtcaggg
     1021 cagaaaaaca aaaatacaga ataaacagga gcacaccgga gtaaaggata acgaaagcag
     1081 cggcaagcat cccgtctgcc gtggtttctt ttaccgtatc ccacgaccag ataattaccg
     1141 gagaaatgac aatcgctgta atcaaaagcc ggataagaaa atcaacaaga cgattagaag
     1201 tgaatatgac tttcatcatt tcccccggtt attttccatt tcttactgtc ctgcctgttt
     1261 cagctggtca gcaatctgct ctttcacttt ctgcgtcaga acgccactgt ccgggatttt
     1321 ctggttattc atcaggtctt cgtagaagtt agtgaaatcc agtctgtcga actgaatttt
     1381 ctgcagctca tcaaccgtaa tcccccgaca gtcaggatgc ttcgcactgc caaacccgat
     1441 acgcaactgc ccgttacgcc cctgctgctg gactatctgc gccagcttcg aatcaaactg
     1501 gcagtagctg cgctttttct gcaggcagac ccccagcacc tttttcgaac agaactcacc
     1561 cacactcacc gtcagtttat tgtcctttgc ctttcccagc gccttttctt cactgttgca
     1621 cttcgccagc cccacatcct gaccccagcc gccatcctta cagcagttac tgaagccggc
     1681 tgcggcctta cggcagaatt tcgcctcacc cgtgaaagcc ctgacatcca caccattcag
     1741 ggcggcaaca tccttacctg cggcggcaag cgcagccagc tgtgatactg cttcaccaaa
     1801 atcactgctt tttccgctct gtgccttatc gcattcaccg tcaaggcaga agacatcacc
     1861 accacagacc atcacttttc ctgatgtttt tgactcgcag gagtacgtgg cgtactcatg
     1921 cagacaggtt ccctcatcgg aataaaacgc gcactgacgt gaggccagcg tacaggccgg
     1981 attatcgaca tatttctgac aggtgccgtt atctgcggac tgagtgacat atttatcccg
     2041 gtatgcccag catgcctgtg tgatgctcca gggtttacct tccatcacac ccgttttcgt
     2101 accacccggc tctgaacact cagtgccttt cagcacgcca tccgctttat taaacggaca
     2161 actctccacc cactccaccc ggggaaccca ttctttatca cggaccttca tcctgagttt
     2221 cagcgtaaag gtggaagcac cactgacaag cgattcatag actttcctgt caccattccc
     2281 gtgcgggagg caattaccgt ttgcagtaca gccactaccg gtcagaacct gcccctgtgt
     2341 gacagaaaac ccggagggca caggtatggt gaaagtcccg ctctgtcctg taaccagttg
     2401 cacattaaag gttgtgttca taaagtcgta acgggagtta aggaaatata gccctgcatg
     2461 aaccgatagc gaggcacttt ccactgtccc ggcaacaggt acggtaacgc tgaacaccag
     2521 tttcagaccg ttcatactga accggaactg tgagtgcggt atcgtcacct cacgatattc
     2581 atccgtgtac ttccagtctc cggtgatact ggcagtccgg gagcagtatt cctccaccat
     2641 cgtgtcccgc tcacaggtat agtttgtgaa ctcactgcgg tttatctgct gcgcactgca
     2701 ctgctgcccg gtgtttccca caatgctgtc agcccggttc accacatccc tgccggtctg
     2761 gataaacggc gcatccggtg aaagaatgtc tttcggcttg ttcatgaatg actctgtgat
     2821 agtttttccg gtttcaccgg tcgcccattc cgtggtgccg tcatttttca ggccgccatc
     2881 cccgccggca gtcacgccac cgtaatattt tgtctcgtcc ggattcgcgt tatagccggg
     2941 gatactctcc tgtggcttaa agccctgaat actgctgctt ccctgccctt tgatctggtg
     3001 agcaaaatca gaaccggccc ggtaatcact gttgctgtca gcctgtgcca taccggtaac
     3061 cagagccagt atcagaggta aaatacgttt catttcccgg aatctccttt ccctgccagt
     3121 aaatcatgag ccacctgccg gcagtcaccc gtggcggcca ctttctccag cgcctggccg
     3181 acccgcagat ttccccggat gatgtcgtat ccctgactgc agaacaccac cagtgccggt
     3241 acgctgcgaa tgccgtactg tgaaaacagc gtcgggtcga tctgaacacc atcagttgcg
     3301 ccgtctttca ccagggacaa cacggcttca gcggtggtct tcaggtcatt gttcaccatg
     3361 ccccgcagtg tggccggaat accgtagtgc cgggtttcgc ccagcatccg tttcagcccc
     3421 tcttcgggaa tggagaagga cacaaagtac acggcgccct gctgtggttt atccttctga
     3481 gcagcctgct gtttacggac cagttcatcg agaaactggt tatcggaatg ctgaagggga
     3541 ttcgccctga cctgctgctc tgcccatgcc tgcaactgta tgtccggctt ttcacgtaac
     3601 tgctgactca gtgtttcctg ctgcttcagg aactggcggt tttcagttgt gttcacattt
     3661 tctgaagcat gaacagcaag atgcatccct gctgtcagag caaaaagtgc aaatattttc
     3721 cggttcatga tatttctcac tttctgctat ttacaggcat gaactcatca gaaaaaagat
     3781 aattgagaaa aacagccata aacacacatg taaatgtcac cttcagatat aatctgacaa
     3841 gtgtcacttg ttctgatata tctgccttct tccattcatg aataatttca gcgagaaaac
     3901 tgtcaggtgc aaaataaaat aatataacag gcaccattac aagaccaaaa ggaaataaat
     3961 ttaatgttct gcttatcagc ctgagcagca cagcacctat tttcctgagg tgctgaatat
     4021 atttactgtc cattgttgcc attttcattt cctcttattc attaaaatga tttacaggaa
     4081 tacacagtta cgtttacgcc acatcaggta gccgaagttt ttctttgtgt tgggcggatt
     4141 ttttcccgtc tcccagcgca tcacactgcg cccgaacgga tggcactgtc cgctgtccgg
     4201 atacatattc accatctgat aacgccagcg ttctttgggc aggattgggg acggatattc
     4261 attacagacg gcgttatttt tcccgatggt ttccataatc atgccctgac ggtgcagctt
     4321 gaatgccatg cgttcactga ccagcaggga ggactgcaac ggactggact cattactcac
     4381 ccagccattg aacgggtaca tactgccctg cgaaccggca caccagaaca gaacatcgag
     4441 aggcatatta aaggcactgg caatggcatc tgctgcgcag gccccctgtg ctatcggatt
     4501 ggcaaaaatg acggcttccg ggttgagaat ggtggtcagg ctgctgtccg tccaggtggg
     4561 gtcgatttca gaaagataag cgatatccag gtcaccacct tccagacagc ccagcgatgt
     4621 gatgatgttc agccagtacg tcagcgggta tttgtaccag tgaacgtgat agaacgcccc
     4681 gttcacctgc ttctcatcct ttttcgccgt tccctgtgcc gttttaccaa aagccggcag
     4741 gctgaagccc aggttcacca tgcatcccgg tgaccgggtg acgtccgtca gcgccatcgg
     4801 ctcccagtaa ccaatggcca gcccgatacg cctgaacagc ggcggcggtg ccggacaaat
     4861 ctgaataggc atcgacgggt tcgccgtgtc ggggacctta ccctggctga ctttgatact
     4921 gcccagcgag agcgggaaaa tacagctcca gcagatatcc gtgatcgggt ttacaaaacg
     4981 cccctcacag gcagaatccg cagacgccgc agggacatga ccggcgagaa ggaataacat
     5041 cagcagccac agtcttcgct tcattttctg ccctcctctg ccggaataaa ttccaccttc
     5101 aggaagcgat cgccgggaac ggcgctcacc cgtgccggga cctgatcaat gccgaggcgc
     5161 tggcagagca caccgttctg gtcaaagtaa atacggctgt ccagcgcttt ctgcatctcc
     5221 gggatactgc cctgcacgag gataattttg ctctccagtg tgggcggcgt ctggcgtttc
     5281 atccaggcca cctgcgccgg atcatcgcca ttgatgaaat acagcgtctg gttaaaagga
     5341 acatactgca gcggattcat cacttcaccc tgacgggcaa acacccgccc ttcgttatcc
     5401 cggatatccg cagccagtct gacggacggg tcaaacaacc ggctgctgta tttctccgtg
     5461 cgtccgatac cgggcacggc agggggccgc agactgttac ggatcacccg ctccttaaag
     5521 gcatccattt tccggcccat ctcaccggac tgctccagtg ccgtcaggcg ctgcatgatc
     5581 accgtcagca tgtccggctc ctttaccggc cacagatcgc cccaggtgcc aagatcggcg
     5641 gcggccacac tctgccccca tatcagcagg gcaatcagcc cccggcatct catggttccg
     5701 ctctcattcg ccgggcaata tcctgctgga tttcccgggt gatatccgga gccccctgta
     5761 ccacggcagg cgacaccaga atgaccgcgt ggtgtttctg ctgccacgcc tgcaggctgg
     5821 cttccagtgc cgtgttaaaa cgcgctgaaa gggcttttga ctgtgcttct gacagttgtt
     5881 tctgactggc gctgtcaaaa aatgcatcca cagtctgttt catgttgaag gcggcagtca
     5941 ccggtgcgtt cagtctgaca atgccgtaac tgacggctgc gttcagtaac accattgcca
     6001 ggcatcccgg caccgtccac caccagtggc tgcgtcgggg ggctgtaaca tcagtcgttt
     6061 tttgcgttgt ggtcatgcaa cactcctgta tttctcatgt tcttccagcc aggcttccag
     6121 cgaagccatc tccggccctg acttcttcca cgccagctgc cacaccgcct catgaatact
     6181 cagcccctct ttacgtttct gctgcacaaa ctcaaaatcc gggccgtcag agctgtacat
     6241 ggcgcgactt aacgggtcca caaacaggcg gtgccaggag gaatggtttt ccacctgcag
     6301 caggaaggaa ctgaaccact ggtctctggc cgcaccaaac ttaccaatca tgtcgcgctg
     6361 cagtggctgg aactggtccg gatacagctg gttgtatttt gcgaactcct tcgcactctg
     6421 tttgaggata attttgtagg aggagttacc ccatgccgca cgggccgcac tggaggcctt
     6481 gtcagagtca aagtcgacga tgttctgtgt gatggtgata taagcaccgg tatgacgacg
     6541 ggcggtacgg taacctttct caataaactc accgaccttg taatttttga agtccagcag
     6601 acgccagcct tcatcaatga cgttcagttt cttgaggtta cgcggcgtgc ggtacatcct
     6661 gttctcgatg tagataatca gggagaacat caccgcaacc agcagtgacg gacggtcttc
     6721 cagtccgccc agctccagca ccaccatttt tgcgtcatcc cgcagggacg gctcatcaga
     6781 gttaaaatac tgaccgtaag tgccgttggc agtgtactgg tcaagcagca caatcatttc
     6841 gtccagacgg ctgcggatag tcggtgaccc ggcatactgc tcgctgtcac tggcgttttt
     6901 caggaaatcc accacgtcat caatacgtgc cctgttctct ttggccagcc aggaggccct
     6961 gaccgcctgc agcagcagac cttcatgcac ttcatccagg ttaccgttgg ggctggccat
     7021 cactgacaac tggtcacgga cacgctccgc tgactggtcg atatcggtga tattcgcaaa
     7081 cgggttaaaa cgcagggttt caccgtccag atatacgcca cccatgttct cacacaggga
     7141 cttgtagcca tcccccatgt cgaacaccac ggcaaagccc ccggagtcca gcacgctgcg
     7201 gataaggggc tgtatcagtc cggttttacc ggccccggag gtgccacaga ccgccatgtt
     7261 gtagttggtg ttattcatcc cccggaagaa aatatcgata aacgccagct ggttacggta
     7321 ggtgggtgcc agcagacctg ccggtgtcag ggggttatcc gccactaacg gcatcaggtt
     7381 ggcaacatta aagctctctg cgcgctgaac cacaccggcc tctttcagct gtttaaacag
     7441 ccctttcccg gccataaagg gcaggcaggt caggaaattg cgcatgtggt taaagcgcgg
     7501 tgaaatcagc tcaaaaccgt ttttacggaa gctgttcagg atgtcctgct ccacttccag
     7561 tgccgtttca ttattgtcct tgcagaaggc tgtgatgttg aggaagtagg acacgacaga
     7621 agactgaccg gagcccagcc gctgacgcag ttccccccac tccttcgcct ctttctccac
     7681 ggacggaaac catttggcat aggaggtctt cgactttttc tccaggtcca tgtacttcag
     7741 gttggcttcg ctgtgggttt tcacctggtc ttccaccacc agagtcagcg tcaggatgaa
     7801 cggacaggag atggacagtt ccgggttcag caggttgctg tagttgtcgg ccatattcca
     7861 caggaaggcg atttccgggt tacgggccag atggaaattc aggatgcggg ccgtgctgtt
     7921 cctgccgttc tcacgcaggc ccagcgtcag gtaatcagcc cggactttca ggtcaaaact
     7981 gtcctccaca cactgataat tcagatcaga atacgggtcc agctgacgtc ttttcgggta
     8041 cagggagtcc gggttatgat taatcatctc cccgacaata tcgataaagg cctgtgcatc
     8101 caccgtctgt gtggcgatac tggccccctg taacgacgcc cggatgattt tcaccaggtt
     8161 ttccatttcc agaatgtcag cccggctttt tttcttcgag ggagaacagt acgagaagaa
     8221 tacccggtaa tggcgcaggg tcaggggcag attcatcccc tccggcagcg gaaactgtgt
     8281 cgctgccgcg ttcatataat aggcacgggt aatggcgtta aaccgttcag cctgttcacc
     8341 ggaccaggag aactcacgca gcccgtattc aattctgtca ccaacaagct ggctggactt
     8401 cagatggatg cagaacggca ctccgcgtgg cagtttggtg cgcagcatgt ggtcgagcgc
     8461 ctccacaatg gactcattcg ccccattgat gggaatggct tccagcataa agcccatcgt
     8521 ggtgtcattc atgaacagac cggactcctg gttgtaatca cggtacggca gcagacggct
     8581 gaactgcggg aagctcattt cgcccagaac ttcattggcc tttgcggatt cgtcaggcag
     8641 tttcagtgct gtgacgaggg agttaaccgc ctgagtgacg gcctcaagtg ggttattcac
     8701 atgaattcct tagttgaaaa ataaagcgag aaatgcggta gccatgaagg gaatacctgt
     8761 aattaacgcg ccatttcccg gcgcatatac aaagatttcg gttttctgaa tatcaactca
     8821 tgttcatccg gaattaaatc aatatgttct gcgactgact tatgcataat gttttctctg
     8881 cttttcctga tacgcctgac attcaacgca caacgtcaca ccgggaaata ttttccgccg
     8941 ggcttccgga ataggatttc cgcatgcttc acagagataa acaggaatac catgaacatt
     9001 tattttctgg cgtatccggt ttattcctgt catggtcagt tgttctgtca ctgaatatgc
     9061 ttcatcggct tcatcactca cggaatattc ctcccgacat gttctgacac gctaccgccc
     9121 atcccttttc acgcacaggg cactcgtttt acaggcagat tttgaataca gaaagcccgc
     9181 cggcattgcc ggctgatata caacttcagg gccagttaat taatacgtgg ttttccccac
     9241 gcagacggtt taataacaaa aaacacactg gatggctgat gataaacatc ctgattatca
     9301 atataaggcg ctatccataa tgcagccgtc tgctcacccg ttcttagcgg gcggggaggt
     9361 gttaccggcg caactgaact gaccggaaca atcgttttca cttcccgggc agccgtaaac
     9421 aacggacgtg gtgccagcag tttctgctcc ggaagtgccg tcaccgcagg tctgctgccg
     9481 gacggagggg tcgcggtgac ggtctgcacc ggcattgtcc ggaagttccc ttcagccagg
     9541 cgcggcagtg atgccgcaac cggctttgct tctgaggact gctccagttc tttggccttc
     9601 tcattggcct gctccatcgt catacaggta tcggatgtgg tggcgttaca ctcaaattcc
     9661 gtactggttc cggcacagcc ggaaagtaat aacgttccca gcagaggaat aaataaagaa
     9721 atctgtttca ttacccttct ccttttttat tactctccac aacagcagaa attaactcta
     9781 ctgtcagatt tgtttttcct gcacagggtt gtccggagac tactgtattt cccggagaag
     9841 aaaataagct catcgacata cccttcttca tcacaccgac agctgccata ttaaaattgt
     9901 gcggcaatga ttcagaaccg aattccttca gtgcaggaag tacgattctt gtggtgcagt
     9961 tactgagaac agcattcata aaatcatctg atacgctttt atccggtaca gagcgtgcag
    10021 taataatatt acgcatgttc atttttatct ccgttgtttc tgtttgccct gtcatcaggg
    10081 aaataaacat ggcatttcct gattttttcc gacagagaag tatcttccgt ataaacttcc
    10141 ttcagatgtt catctgcggt tcccacccgg acacatttca tttttccggg tcgcaccgga
    10201 tatttcccgg tgatcgctgc cagcgagaca tacatcagtg ccagtgttgc ccagaatgcg
    10261 gtgaccagtt gcatcagtga ccggtcagtc tccctgacat aatctgcagc atcagccgcg
    10321 cggtaatggc agatatcctg tatttctgat ctgcctgaag aatcagagga agaaaaccag
    10381 cattcactga ccgggaactg tccggcaggt gctgtttgtc ttacgaagtt aatctcctta
    10441 accagtaatt tccccagcgt ggtgttgtcc ttccataaaa caaacagagc catcagggcc
    10501 attgtggcca gcggccagat aaccacaaac ctgacgaccc acagcagcca gcgaacgaca
    10561 aacgccacac ggaatgtcag tgcatcaccc gccatttccg ccctccttgc cggcatcccc
    10621 gccctgcggg ttcttgtctc cggacataaa ctgctgcgga ttcagattgc ccagctgctt
    10681 cagcatctgg tcggtgttaa aaccgttcag gtggctttct gccgagggag gattcggcac
    10741 agggctttcc gggttaccct cttccgctgt actctgtgac tgctccagcg ccatttcctc
    10801 cacggttttc agctggaagc cgtcctggaa caccactgtc acttcgttgc ccgcaccaat
    10861 cggtatcacc gggtgatact gttcggcacg tttgatgtag tagtcactga gcgtctgtgc
    10921 ggctttcgat gcgccgccac cgatacccat tttaaggaca tcgccggccc cgtaagcggc
    10981 tgtggcaccc agcccgacag ccggctggga ggcgcgctcc attccctgac cgataccgtc
    11041 aacaaatccc gcgccccatg cccagccgag gattttgccg ttacgcatca ccacttcgcc
    11101 cttgataccg tttttacccc ggaagctgac atgcccctta atcggcatat caatggtttt
    11161 gccattcttc aggcagctga tattacgggt gcgaacaata gcacgttcac tggagacatc
    11221 cccccaggct tccagcccca caaaacatcc cgttgcgtca tacgtcttgc tgttcggcat
    11281 ttccaccaga ccggtgatac gcagctgcat cggcaccgtg gattcattac cggtgactga
    11341 ggcattggca tccgcccctt caatcagcat ggctttcgca aaacttcctg acggaatgta
    11401 cggcagcgat ggtccctgtt ttccctcatt acgggtaaac accttacgct gtatccggtt
    11461 aggcaccggc acagactggt acatcacctg tggtggtggc gtgacaccat tcccgggata
    11521 aaacgccgtc ggcggtggaa cagcaacgct gccctgcggc gggaaggata cgggggtgtt
    11581 tcccggctgt ggttcgcctt ccggcccggg cggtgaggct ggcgtctgcg gtacaggctc
    11641 accggtcgcc gtgacgggat tagcacccag ggcttttacc tgctctgcca gggcggcatt
    11701 gtcctgtccc agtttttcaa tacgacgctg gtcatcaccg cgctgtttgt tcaggacatc
    11761 cagttcacga cggatttcct catactgctt ctgcatctgc gctgccgtca cctgcatctc
    11821 tgtggtggca tgctgacgca ccttgtcatc aaaggtcgta tccaccacac cggtcatatc
    11881 cggcacaggc tcctgttcag ccacggtttc cccgttaccg gacatgtcca catcagacag
    11941 atacagtgcc ccaccaatcg cggaggctgt accgacaacc acaatcccca gccacaggta
    12001 ctgcttgcgt ttcacaatgg tgttgatact ggccatcagt tgccctcccc gtcacggatg
    12061 acatacacat ccatgcacgc acctgccagt aactggctgg caggctggct gaacatcacg
    12121 gcacgggttc ccggctgcca gaagtcactt tcccggatat tcagggcaga cagcgtttta
    12181 ttttccacgg caaagcggac catcttcagg tgattccccg tccatacggc atctgccact
    12241 gaagacagcc ccgccggggc ctgcagggtt tcctttgtca caggaacctg ataccagcct
    12301 gcaggtaatt ttccgccacg gacagcctgg ctgatggtca caagcaggga ttcgtaaggc
    12361 gtggacgttt cccacgcacc ggcttcttct ccggttccgc gcaggtcact gaccagctga
    12421 atggtacgcc ccgcgccttc acggggaacg gcctgaatgg aaagattcag accacgttct
    12481 gtttccagaa tgaacgtaaa gggctttttg ttgacggtgg caaccaccac accgccggag
    12541 gcggtctgct cattattggt cagggcacca tccagactgt tcacggcgat aatccggtca
    12601 ccgggaacgg taaacagatt cgggctggta ttgctgatgg caatactggc ctgaccaccg
    12661 ttcaccattg gcaccacggt gggggacagc gtaccgtttg cggccatcac gctgcaggaa
    12721 aaaaacaggc tgccgaatat tattgccgta ttattttttc tcatcgtctg tttccccgaa
    12781 attatccagc caggtcaccc cgttttcccg cttcagaata aggatgtaat gtttgatatc
    12841 agtgaaaggt tttgaatcac caatccaggt tttaagcaca ccacgaattt ccacacggcc
    12901 atactgaggc cagacacgaa cactggtctg gaaaaaggct gagttcacgt tatcgttttt
    12961 aatacgcttc gcttcttcag ccagaataac tttcatctgg ttctgtgcgc ccgggcggat
    13021 atatttcaga agcgcctgat gtgaggcatc gacggtttct gatgaaacat tcagacggag
    13081 ggcaataaat gacagcgcca tctgctgtaa ataagaggcg tcggcactgt tctgtgacac
    13141 ggcaaagggg gcattaaatg ccattggcgt cacggcagtg cgttgctcat tctgaagacg
    13201 gtagttattc accccctgaa tgacgttaac agagaggctg agaacaataa gcactgacat
    13261 aaatataaag gcgatggcca ttacacgact ggtacttaaa cgggcaccgt gttccatata
    13321 acaatcctgg tatcagttct atttaaccca ctgccggaaa cacgaatcgg gaacattatg
    13381 aaaaataccg cgcagcaggg ctgttggcat ataccagtaa atcaggtctc gtaaccagga
    13441 actgccccgc ccttttttca gttttttaat cccgaaataa accagaaccg ctgcaccaat
    13501 accgaacaga tattttgatg ttgtgatacc ccagccaata cagattgcgg cggggatcag
    13561 ttcatccagt ggcaggccaa accagcggct ctggttggtc agtgtttccg ggaaacgata
    13621 tttcttaagt ttattctcgt ctcccgacat cgttttattt caggccaacg acggccatac
    13681 ccacagcaat aaatacagag atgatggcaa aaccggccag gaacttgacg tttttggtca
    13741 tcatgtacat gaccgcaccg accagaactt cagccagaac aacccattta acaacactgg
    13801 agtccttacc gaaggtcgcc ttaaccgtgg tgttaccgct tgccatcagg tcctggccct
    13861 gtgctgccat tgccagttgc gggaagaaca tcatcagaac agcagcaggg atcacagcgc
    13921 gagccaggcg aagcatattc agacgagtga atttagaaaa aaacgatttc tttttgacgg
    13981 gcgcagaagc accctgaaca cttaaaaagg cgtccatatg attcctctgt ttaatattga
    14041 tagataaaaa ctaaagttct ttaaatgttg actcgttctc ttcgatcacc tccctgaata
    14101 tttcttcatt atagaaatca ggaaaccgtt tcagatgatc tctgagacgg atctgaacct
    14161 ctatagtttt acttctaccg ctacgatctt tggcccgaat aagtcggtta tttgtatctt
    14221 catcgagata cactgagact gttctgctta tcccgcctct cgcgttacgc ctcctcactc
    14281 aattcacctc tgtttcctga ccaataaaat catgattgca cagaaatggc ctgtcgaaaa
    14341 tgggcccaaa acgagccctg ctttattaca tttttttaac acccatatat tcactcaaaa
    14401 catttaatat ttcgtacatt acacctgtct taatacattt attcctgaac aaatcgaagg
    14461 aacccaatct tttattgata tccgcattaa tcttttt
//
LOCUS       DACADF010000117        14357 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.119, whole genome shotgun sequence.
ACCESSION   DACADF010000117 DACADF010000000
VERSION     DACADF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.119"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            149..1183
                     /locus_tag="ISB30_003472"
     CDS             149..1183
                     /locus_tag="ISB30_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP1991072.1"
                     /translation="MAKSDTGRKRVTLTDVARAAGVSKSTVSLVLNDSSLIKKETQQK
                     VQQAIEQLGYVYNRFAANLRSQKSLTIGVVIDDLINPFFAEFTMGLEMTLAEHGFITV
                     MSNTSQRSDRQKQVLDTLLEHHVAGIVLCPVNSTSEADLQRYANSSTPLLITMRPLDW
                     QQLPVDYVGVDSHAGVREATEYLIQQGHRDIVFIGGLTHHMRYQGYLEAMNHHGLQPW
                     STDAFSLRAEPTQANGYQLMQQLLDMPSPPTAVICYNDLMAFGAESALGERGLFAGED
                     ISLIGNDGVAACAYSNPPLTTIAVEPLALGKQAAQQILRRIAQPDAPLSHYIYRPTLQ
                     IRASTGKRGR"
     gene            1256..2671
                     /locus_tag="ISB30_003473"
     CDS             1256..2671
                     /locus_tag="ISB30_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAP1991073.1"
                     /translation="MAGNRPFNKQQTDNRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            2724..3803
                     /gene="alr"
                     /locus_tag="ISB30_003474"
     CDS             2724..3803
                     /gene="alr"
                     /locus_tag="ISB30_003474"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP1991074.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEANLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGAPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            3826..4383
                     /locus_tag="ISB30_003475"
     CDS             3826..4383
                     /locus_tag="ISB30_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP1991075.1"
                     /translation="MSYSEIAACLAYAVSVWLAARNNVHTWWIGIIGSILYGWVFWSV
                     QLYADVTLQLFFIVTSITGWIHWLKGQGGDILPVRRTQASHFFLLLLCAVVVAGGYGF
                     LLHTFTNAWAPWLDSLILTFSVLAQFMLMGRRIENWYVWLAVNTLAVPLYMTRGLNLT
                     AGLYFLFWINAWYGLYQWRKELQTS"
     gene            4380..5384
                     /locus_tag="ISB30_003476"
     CDS             4380..5384
                     /locus_tag="ISB30_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP1991076.1"
                     /translation="MKPFATGLVVGKFAPLHCGHEKLINTALAQCEELFIISYSVPEM
                     PDCEPEKRLTWLQVRFPQATFLVLTPELVARYNLPAIPHNNADADIHRHYVATLCLQI
                     LRCRPHAVFTAEDYGDGFANVLARRFAQPVEHVRMARPVGDEAPSGTLIRSDVHRYRY
                     MLANDVYYSFVRRICLLGGESTGKSTLSKALAGVLDTVYVAEFGRDYWEEKNGILTAD
                     DLLHIACEQVRREQQAEANHYLICDTSPLTTLFYALDQYGHAPQELHQLAEREYSLVV
                     LCGAEFPFVQDGTRQGEVFRARQQVWYEQELSRRNIPYLSVSGPLQERVEQILRQLPD
                     "
     gene            5466..6659
                     /gene="tyrB"
                     /locus_tag="ISB30_003477"
     CDS             5466..6659
                     /gene="tyrB"
                     /locus_tag="ISB30_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAP1991077.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESSVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNEQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLVEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDQLREEFGVYLIASGRMCVAGLNTQNVHRVAKA
                     FAAVM"
     gene            6841..9585
                     /locus_tag="ISB30_003478"
     CDS             6841..9585
                     /locus_tag="ISB30_003478"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAP1991078.1"
                     /translation="MENMTSPGTLLSGDNATWLEEYYQTWLRTPEQLPEDWRRFFLSP
                     ELTVQSVSGDNNISGATLKKQAAVIQLINAWRTQGHLRAKLDPLGLNPPADVPSLQPG
                     FWGLSEEDLLQEFSVTFGAHTTQMPLKQLLNLLEQAWASSQAYELAHLENREEINWLL
                     SRIESSNAPQADAQTCIARFEKLMAAETLERYLHTRYVGQKRFSLEGGESAIPALDTL
                     TKRLRAQGVEEMVIGMAHRGRLNVLVNLLNKDPAQLFAEFEGKQTIGSGSGDVKYHMG
                     YSSNLETPAGSLHVALAYNPSHLEIVNPVVLGQVRARQERRGEDGQAKVVGVLIHGDS
                     ALGGLGVNQTTFNLSQTQGYGTGGTLHLVINNQIGFTTSRLQDMRSSRYCTDIAKMVA
                     APIIHVNGDDVDAVCQVMELACEWRDTFRRDIIIDICCFRKHGHNESDEPRLTQPQMY
                     QAVDAHPGTLARYGESLARRGLLTQAQQDEMTARYRDWLDSCQKREPQPLKPAIHSFS
                     ANWYGLTNPHWSAPVSTALPRQKLAAYGEIISTLPPDVVAHPTIKRQLALRQDMAAGT
                     QPVDWGMAEMLAYASLVDAGVGVRLSGEDSGRGTFSHRHAVVHHQTEARRYLPLQHIR
                     AGQASFDVYDSVLNEEALLAFEYGYSTSAPQQLVIWEAQFGDFANGAQVAIDQFISSG
                     ETKWDRYSGLTILLPHGYDGQGPEHSSARPERWLQLCAENNMQVVMPSESAQMFHLLR
                     GQALRPMRKPLVIMMSKRLLRFKGAMSELCEFTDDAYKPVITDPQLHQSQKVKRVILC
                     SGQVYYDVLEARKQREHEDEVAIVRLEQLYPFPVAELNDVLASWPNCCEWIWLQEEPE
                     NQGAWRQIRHELAALKINTPYWQYAGRPAAAAPATGYGRVHKQQIDEFLAAAFADIQP
                     "
     gene            9618..10772
                     /gene="odhB"
                     /locus_tag="ISB30_003479"
     CDS             9618..10772
                     /gene="odhB"
                     /locus_tag="ISB30_003479"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAP1991079.1"
                     /translation="MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVI
                     LEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQ
                     RSGVELADVAGSGRNGRILKEDVQRVTPAPAIQPERVAEIAPAKPLTPGARQERREPM
                     SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFV
                     KAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIER
                     QIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRP
                     VAENGQVVIRPMMYLALSYDHRIIDGQEAVHTLVAIRELLESPEQLLLDL"
     gene            10784..12202
                     /gene="lpdA"
                     /locus_tag="ISB30_003480"
     CDS             10784..12202
                     /gene="lpdA"
                     /locus_tag="ISB30_003480"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP1991080.1"
                     /translation="MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPS
                     PGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRL
                     TMGISLLFKKNKVKHLCGLATLECAQDEIWQLRVNDQHLHARNVVIATGSQPRQLPGV
                     TIDNQQILDNRGALALSEVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPA
                     LEARLSNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAI
                     GRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGV
                     VVADQIAGLVVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGR
                     ALALGQEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHA
                     HPTLSEVIHEAAMALNNKALHG"
     gene            12224..13393
                     /gene="sucC"
                     /locus_tag="ISB30_003481"
     CDS             12224..13393
                     /gene="sucC"
                     /locus_tag="ISB30_003481"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAP1991081.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFAAFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEASITLPVVVRLSGNNAAEGQRLLAESGLTVEAVDSLDDAAKRIIALLN"
     gene            13406..14278
                     /gene="sucD"
                     /locus_tag="ISB30_003482"
     CDS             13406..14278
                     /gene="sucD"
                     /locus_tag="ISB30_003482"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAP1991082.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKDAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRHDMNKPVVGYIAGVTAPKGKRMGHAGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWNVIK"
BASE COUNT         3405 a         3489 c         3988 g         3475 t
ORIGIN      
        1 gattagggtg taaaaaacac aacaagggaa gtgcgaatct gtgatcgaaa cgacgatctc
       61 ctgcgatccc ttgcgttaac ggatcgcatg agtaaaagga gattatgata aagtaattgc
      121 cttgatctta tgggggtttg gagcaggcat ggcgaagtcg gatacaggaa gaaagcgggt
      181 aacattaacc gatgttgcgc gtgcggctgg cgtttcgaaa tcgactgtat cgctggttct
      241 caacgacagt tcacttatca aaaaagagac gcaacaaaaa gtccagcaag ccattgaaca
      301 attaggctat gtctataacc gttttgctgc caatctgcgt tcgcaaaaat cgctaactat
      361 cggcgtagtc attgacgatt tgataaatcc tttcttcgct gaatttacta tgggtcttga
      421 gatgacgctg gcggagcacg gttttatcac cgtgatgtcc aatacctccc agcgtagtga
      481 ccggcaaaaa caggtgcttg ataccttgct ggaacaccac gtcgcgggca ttgtcctctg
      541 tccagtgaac agtacctcag aagccgattt acagcgttat gccaacagtt ccacaccctt
      601 attaatcacg atgcgccctc tggattggca acaattgccc gtcgattacg ttggcgtgga
      661 cagccacgca ggtgtacggg aagcgacgga atatctcatt caacaagggc acagagatat
      721 cgtgtttatt ggtggtctta cgcaccatat gcgttatcag ggataccttg aggcgatgaa
      781 tcaccatggt ttacagccgt ggagtactga tgctttttcg ctgcgtgctg aaccgactca
      841 ggccaacggc taccagttga tgcaacagct gttggatatg ccttcgccac ccacagcggt
      901 gatttgttat aacgatttaa tggcttttgg cgcggagtcg gcactaggcg aaagaggatt
      961 atttgccggg gaagatattt cgttgattgg taatgatggc gtggcggctt gtgcttacag
     1021 taacccgccg ttaaccacaa ttgcggtaga accattggct ctggggaaac aggcggcgca
     1081 gcaaatttta cggcgtattg cgcaaccaga cgcaccgctc agccattaca tctatcgccc
     1141 gaccctgcaa attcgcgctt ccaccggaaa gcgggggcgt taaatgctgt agcgagaaac
     1201 tgtttctcgc tatactctct tgtcccccat tcaacagcag gtaacaccat tcgttatggc
     1261 aggcaataga cctttcaaca aacaacagac tgataaccgc gaacgcgatc cacaagttgc
     1321 cgggctgaaa gtgcctccgc actcgatcga agcggagcag tcggtgttgg gcggtttaat
     1381 gctggataac gaacgctggg atgatgtagc cgagcgtgtg gtggcagacg atttttacac
     1441 ccgcccacac cgtcatatct ttactgaaat ggcgcgtttg caggaaagcg gtagtcctat
     1501 cgatctgatt acccttgcgg aatcgctgga acgccagggg caactcgata gcgtcggtgg
     1561 ttttgcttat ctggcagagc tgtcaaaaaa tacgccaagt gcggcgaaca tcagtgccta
     1621 tgcggatatc gtgcgcgaac gtgccgttgt ccgtgagatg atctccgttg cgaatgagat
     1681 tgccgaagcc ggttttgatc cacaagggcg taccagtgaa gatctgctgg accttgctga
     1741 atcccgcgtc ttcaaaattg ccgaaagtcg tgcaaacaaa gacgaagggc cgaagaacat
     1801 cgccgatgtg ctcgacgcaa ccgtggcgcg tattgagcag ttgtttcagc agccacacga
     1861 tggcgttacc ggggtaaaca ccggttatga cgatctcaac aaaaaaaccg ctggcttgca
     1921 gccgtcggat ttgatcatcg ttgccgcgcg tccgtcgatg ggtaaaacga catttgcgat
     1981 gaacctcgtc gaaaacgcgg cgatgttgca ggataaaccg gtacttatct tctcgctgga
     2041 gatgccatca gaacagatta tgatgcgttc tctggcgtcg ctgtcgcgtg ttgaccagac
     2101 taaaatccgt accgggcagc tcgatgatga agattgggca cgcatttccg gcaccatggg
     2161 tattttgctc gaaaaacgca atatctatat cgatgattcc tccggcctga cgccaacgga
     2221 agtgcgttcc cgcgcacgcc gtattgcccg tgaacacggc ggcatcgggc ttatcatgat
     2281 cgactacctg caactgatgc gcgtaccggc gctttccgat aaccgtacgc tggaaattgc
     2341 agaaatctcc cgctcgctga aagcactggc gaaagaactg aacgtgccgg tggtggcgct
     2401 gtcccagttg aaccgttctc tggaacaacg tgccgacaaa cgcccggtca actccgacct
     2461 gcgtgaatct ggctctatcg agcaggatgc ggacttgatc atgtttatct atcgtgatga
     2521 ggtgtatcac gaaaacagtg atttaaaagg catcgcggaa attattatcg gtaaacaacg
     2581 taacggccca atcgggacgg tacgcctgac ctttaacggt caatggtcgc gcttcgacaa
     2641 ctatgcgggg ccgcagtacg acgacgaata ataattattt tatgaattag gtaattaaag
     2701 caaacactta tcaaggaaca caaatgcaag cggcaactgt tgtgattaac cgccgcgctc
     2761 tgcgacacaa cctgcaacgt cttcgtgaac tggcacctgc cagtaaaatg gttgcggtgg
     2821 tgaaagcgaa cgcttatggt cacggtcttc ttgagaccgc gcgaacgctc cccgatgctg
     2881 acgcctttgg cgtagcccgt ctcgaagaag ctctgcgact gcgtgcgggg ggaatcacca
     2941 aacctgtact tttactcgaa ggcttttttg atgccagaga tctaccgacg atttctgcgc
     3001 aacattttca taccgccgtg cataacgaag aacagcttgc tgcgctggaa gaggctaacc
     3061 tggacgagcc ggttaccgtc tggatgaaac tcgataccgg tatgcaccgt ctgggcgtaa
     3121 ggccggaaca ggctgaggcg ttttatcatc gtctgaccca gtgcaaaaac gttcgtcagc
     3181 cggtgaatat cgtcagccat tttgcgcgcg cggatgaacc aaaatgtggc gcaaccgaga
     3241 agcaactcgc tatctttaat accttttgcg aaggcaagcc tggtcaacgt tccattgccg
     3301 cgtcgggtgg cattttactc tggccacagt cgcattttga ctgggtgcgc ccgggcatca
     3361 ttctttacgg cgtatctccg ctggaagatc gctccaccgg tgccgatttt ggctgtcagc
     3421 cagtgatgtc gctaacctcc agcctgattg ccgtgcgtga gcataaagcc ggagagcctg
     3481 tcggttatgg tggaacctgg gtaagcgaac gtgatactcg tcttggcgta gtcgcgatgg
     3541 gctatggcga tggttatccg cgcgccgcgc cgtccggtac gccagtgctg gtgaacggtc
     3601 gcgaagtgcc gattgtcggg cgcgtggcga tggatatgat ctgcgtagac ttaggtccac
     3661 aggcgcagga taaagccggg gccccggtca ttttatgggg cgaaggtttg cccgtagaac
     3721 gtatcgctga aatgacgaag gtaagtgctt acgaacttat tacgcgcctg acttcaagag
     3781 tcgcgatgaa atacgtggat taatattcgt ctccagggag ggacaatgtc ttattcagaa
     3841 atcgccgcgt gtctggcata cgcggtttcc gtctggctgg ctgccagaaa caatgtgcat
     3901 acatggtgga tcggcataat tggcagcata ttgtacggct gggttttttg gtccgtgcaa
     3961 ctctatgccg acgttacgct ccagttattc ttcatcgtga ccagcatcac tggctggatc
     4021 cactggctga aaggtcaggg tggcgacatc ttgccggtgc gccgaacgca agccagtcac
     4081 tttttccttt tgttgctctg tgctgtcgtc gtggcaggcg gttacggctt tttactccac
     4141 acctttacca atgcttgggc accctggctg gattcgttga ttttgacctt cagcgttctg
     4201 gcacaattca tgttgatggg aagacgtatc gaaaactggt acgtctggtt agcggtgaat
     4261 accctggcgg tgccactgta tatgacgcgc ggtttaaacc tgaccgctgg cttatatttc
     4321 ctgttctgga ttaacgcctg gtatggtttg tatcaatggc gcaaagagtt gcaaacatca
     4381 tgaagccttt tgcaacgggt ctggtggttg gtaaattcgc gcctttgcat tgtggtcatg
     4441 aaaagttgat caataccgca ctggcgcaat gtgaagaact gttcatcatc agttattccg
     4501 tgccggaaat gcctgactgt gaacctgaaa agcgcctgac gtggctgcaa gtgcgcttcc
     4561 cgcaagcgac gtttttggta ctgacgccag agcttgttgc gcgttataac ttacctgcta
     4621 tcccgcataa caatgctgat gccgacatcc accgtcatta tgtggcgacg ctatgcttgc
     4681 agatattacg ttgccgccca cacgctgtat ttactgcaga agactatggc gatggctttg
     4741 ctaacgtgct ggcgcggcgt tttgcccaac ccgttgaaca cgttcgaatg gcgcgcccag
     4801 ttggcgatga agcgccttcg ggaacgctta ttcgttccga tgttcaccgc tatcgctaca
     4861 tgttggcgaa tgatgtttat tacagtttcg ttcgccgtat ctgtctgctt ggtggcgaat
     4921 ctaccggtaa aagcacgctt tcaaaagcgc tggcgggcgt gcttgatacg gtgtatgtcg
     4981 ctgaatttgg ccgcgactat tgggaagaaa aaaacggtat cttaactgcg gatgatttgt
     5041 tgcatatcgc ctgcgaacag gtacgccggg aacaacaagc agaagctaat cactatctta
     5101 tatgtgacac gtcgccactc acgaccttat tctatgccct tgatcaatat ggtcatgctc
     5161 cgcaagaact tcaccaactg gcggagcggg aatactctct ggtagtgctt tgcggcgcag
     5221 aatttccctt cgtgcaggat ggcacccggc aaggagaggt atttcgcgca cggcaacagg
     5281 tgtggtatga acaggaattg tcgcgcagga acattcctta tctttcggtt tcggggccac
     5341 tgcaagagcg agtggagcaa attcttcgcc aacttcccga ctgactttga acatcctctc
     5401 gatcttcgcc ttcttccggt ttattgtggt ttaaccacct gcccgtaaac ctggagaacc
     5461 atcgcgtgtt tcaaaaagtt gacgcctacg ctggcgaccc gattcttacg cttatggagc
     5521 gttttaaaga agaccctcgc agcgacaaag tgaatttaag tatcggtctg tactacaacg
     5581 aagacggaat tattccacaa ctgaaagccg tggcggatgc ggaagcgcgc ctgaatgcgc
     5641 agcctcatgg tgcttcgctt tatttaccga tggaagggct taacagctat cgccatgcca
     5701 ttgcgccgct gctgtttggt gcggaccatc cggtactgca acaacagcgc gtagcaacca
     5761 ttcaaaccct tggcggctca ggggcattga aagtgggcgc agatttcctg aaacgctact
     5821 tcccggaatc aagcgtctgg gtcagcgatc ctacctggga aaaccacgta gcaatattcg
     5881 ccggggctgg attcgaagta agtacttacc cctggtatga cgaagcgact aacggcgtgc
     5941 gctttaatga cctgttggcg acgctgaaaa cattacctgc ccgcagtatt gtgttgctgc
     6001 atccatgttg ccacaaccca acgggtgccg atctcactaa tgagcagtgg gatgcggtga
     6061 ttgaaattct caaagcccgt gagcttatcc catttcttga tattgcctat caaggatttg
     6121 gtgccggtat ggaagaggat gcctacgcca ttcgcgccat tgccagcgct ggattacccg
     6181 ctctggtgag caattcgttc tcgaaaattt tctcccttta cggcgagcgc gtcggcggac
     6241 tttctgttct gtgtgaagat gccgaagccg caggccgcgt actggggcaa ttgaaagcaa
     6301 ctgttcgccg caactactcc agcccgccga attttggtgc gcaggtggtg gctgcggtgc
     6361 tgaatgacga ggcattgaaa gccagctggc tggtggaagt agaagagatg cgaactcgca
     6421 ttctggcaat gcgtcaggaa ctggtgaagg tattgagcac agagatgcca gaacgcaatt
     6481 tcgattatct gcttaatcag cgcggcatgt tcagttatac cggtttaagt gccgctcagg
     6541 ttgaccaact acgtgaagaa tttggtgtct atctcatcgc cagcggtcgc atgtgtgtcg
     6601 ccgggcttaa cacgcaaaat gtgcatcgcg tggcaaaggc gtttgctgcg gtgatgtgat
     6661 tttaatcaac aggcggatct ctccgcctgt tcctgactcc agccactccc tactattttg
     6721 tccctttttt cgcccacttt tacatcatct cttttctata agtttttgaa aattagtgtt
     6781 tttaactgtt ggtacggatt gtgcagtaat gcagctgtct atgaccatca ggagaaaaac
     6841 atggagaaca tgacctcgcc cggcacgctg cttagtggag ataacgcgac ctggcttgaa
     6901 gagtactacc agacctggtt gcgtacgccg gaacaactcc cggaagactg gcggcgtttt
     6961 tttctttcac ctgaactgac tgtccaaagc gtatccggtg ataacaatat cagcggtgcg
     7021 accttaaaaa aacaggctgc ggtaatacaa ctgatcaatg cctggcgcac gcaggggcat
     7081 ctgcgcgcaa agcttgatcc acttggtttg aatcccccgg ctgacgttcc ctccctccag
     7141 cctggttttt ggggattaag cgaagaagac ttgttgcagg agtttagcgt cacctttggc
     7201 gcacacacca cgcaaatgcc acttaagcaa ctgcttaacc tgctggagca agcctgggcc
     7261 agtagccagg cttatgaact tgctcatctg gaaaatcgtg aagaaatcaa ctggttgtta
     7321 tcacggattg agagcagcaa tgcgccacaa gctgatgccc aaacctgtat tgctcgtttt
     7381 gaaaaactga tggcggcaga gacgttagag cgttatctgc atacccgtta tgttgggcag
     7441 aaacggtttt ctctggaagg gggcgaaagt gcaatcccgg cgctcgatac gctgacaaaa
     7501 cgcctgcgtg cgcaaggtgt ggaggaaatg gtcattggca tggcgcaccg gggaagactt
     7561 aatgttctgg ttaatctgtt gaataaagac ccggcacagc tttttgctga gtttgaaggc
     7621 aagcagacga tcggcagtgg ttcaggcgat gtgaaatacc acatggggta ttccagcaat
     7681 ctggaaacac ctgccggttc gctgcatgtg gcgctggcgt ataacccctc acatctggag
     7741 atcgtgaatc cggtggtgct gggccaggtg cgagccaggc aggaacgccg tggtgaggat
     7801 ggtcaggcaa aagtggtggg cgtacttatt catggggatt ccgcgttggg cggcctcggc
     7861 gtcaaccaaa ccacgtttaa cttatcgcag actcagggct acggcactgg cggtacgcta
     7921 catctggtga ttaacaatca gattggcttc accacttccc gcttgcagga tatgcgttct
     7981 tcacggtact gcaccgatat tgccaaaatg gtagcggccc caattatcca cgttaatggc
     8041 gatgacgttg acgctgtttg ccaggtaatg gaactggcgt gcgaatggcg agacaccttc
     8101 cggcgtgaca tcatcatcga tatttgctgc ttccgtaagc acggacacaa cgaaagcgat
     8161 gagccacgtc ttacccagcc acaaatgtat caggcagtgg atgctcaccc tggtacgctc
     8221 gcccgttatg gtgaatctct tgcccgccgc ggcctgttaa ctcaggcgca acaagatgag
     8281 atgacggcac gctatcgcga ctggctggat agctgccaga aacgtgaacc gcagcctctc
     8341 aaacctgcaa ttcactcttt tagcgctaac tggtatgggt tgaccaaccc tcactggagc
     8401 gccccggtat ctacggcatt gcctcggcaa aagctggcag cgtatggtga aatcatttct
     8461 acactgccac ccgatgtggt cgcacatcct accattaaac ggcaactggc gttacgccag
     8521 gatatggccg caggtactca gccagtagac tggggaatgg ctgaaatgct ggcctatgca
     8581 tcgcttgtgg atgcgggtgt tggtgtgcgt ctttctggtg aagactcagg gcgtggcacg
     8641 ttcagtcatc gccatgcagt ggtacatcat caaacggaag cccgtcgtta tctgccgtta
     8701 cagcatatcc gcgctggtca ggcatctttc gatgtttatg attcggtgct taatgaagaa
     8761 gcgctcttag cctttgaata tggctattca acgtctgcgc ctcagcaact ggttatttgg
     8821 gaagcacagt ttggtgactt tgccaacggc gctcaggttg ctattgacca atttatctcg
     8881 tctggcgaaa ccaagtggga tcgctatagc ggtttaacca ttcttctgcc gcatggctat
     8941 gacggacaag ggccggagca ctcttccgca cgtcctgagc gctggctgca actgtgcgca
     9001 gaaaacaaca tgcaggtggt gatgcccagt gagtcagcac aaatgttcca tttgttgcgc
     9061 ggtcaggcat tacgcccaat gcgcaaaccg ttagtgataa tgatgagtaa gcgtttactt
     9121 cggtttaaag gcgcgatgag cgagctttgt gaattcactg atgacgctta taaaccggta
     9181 atcaccgatc cgcaattgca tcagtcccag aaagttaagc gcgttatttt gtgtagcggc
     9241 caggtctatt acgacgtact ggaagcccgt aagcagcgtg agcatgaaga cgaggtagcg
     9301 attgttcgcc tggagcaact ctacccgttc ccggtcgcag aactgaatga tgttctggcg
     9361 agttggccga actgctgcga atggatttgg ttgcaggaag agccagaaaa ccagggcgcg
     9421 tggcggcaga ttcgtcatga gctggcggca ctgaaaatta acactcctta ctggcagtat
     9481 gcaggccgcc cggcagcggc agcaccagcg acagggtatg ggcgtgtaca taaacaacaa
     9541 atcgacgaat ttcttgctgc tgcgtttgca gatattcagc catgaaaggc gcacaaaaat
     9601 aataaggaga acattttatg atcgaaatta ctgtccctgt attaccggaa tctgtcacgg
     9661 aaggcacgct gacaacctgg tgtaaacaag aaggcgagca cgtaaaacgc gatgatgtga
     9721 ttgccgagct ggaaaccgat aaagtcattc tggaaatacc ggccccgcac gatggcgtgt
     9781 taagcaatat tatcgtcagc gaaggtagca cggtcacgtc cgcgcaactg ttggcgcatc
     9841 ttaagccgca agctgtcata gaagaaaccg tcacaccagt caccgaaacc ctggcgatgc
     9901 cttccgcacg gctggaggca cagcgtagtg gtgtcgaact tgctgacgtt gcggggagcg
     9961 ggagaaatgg acgtatcctg aaagaggatg tgcagcgcgt tactcctgca ccagccattc
    10021 agcctgaacg ggtggcggag atcgcgccag ctaaaccctt gacaccgggt gctcgtcagg
    10081 aacgtcgtga gccgatgtcg cgcttacgtc agcgaattgc tgagcgtctg ctggcttccc
    10141 aacaaaataa cgccattctt accacattca acgaagtaaa catgcagagc gtgatggact
    10201 tacgcgcccg ctggaaagat cgttttgccg agaagcacgg cgtgaagctg ggctttatgt
    10261 ccttctttgt taaggccgtt acccgggcgc tggagcgctt ccctgtagtg aatgccagcg
    10321 tcgatggcaa cgagattatc tggcgtgatt attgcgatat cggcattgca gtgagcagca
    10381 accgcggcct ggtagtgcct gtgttacgca atgcgcaatc actctctttg gtggaaattg
    10441 aacggcaaat tgccgaatac gccacacagg cacgcaatgg caaattgccg ctggaggcgt
    10501 tacagggcgg cacgttctct atcaccaacg gcggtacttt tggttcgatg atgtcaacgc
    10561 caatcattaa cccgccgcag tcggccattc ttgggatgca tgccatcacg ccgcgcccgg
    10621 tcgcagaaaa cggtcaggtt gttattcgtc ccatgatgta ccttgctctc agctatgacc
    10681 atcgcattat cgatggtcag gaagccgtgc atacattggt ggcaatacgt gagctactgg
    10741 aatcaccgga acaactgttg ctggatcttt aaggagggcg ccgatgagta caatttttga
    10801 tgtggcagtt atgggggggg ggccaggtgg ttacgtagcg gcactgcggg ctgcgcagaa
    10861 tgggctttcc gtagtatgta ttgatgatgg tgttaatgca cagggcgaac cttcgccggg
    10921 cggtacgtgt ctcaatgtcg gctgtattcc gtcaaaatcg ttgctgcaat cttccgaact
    10981 gtacgcgcaa gtgcagcatg aggcgagcat tcatggcgtt aatgttgacg gcgtatcttt
    11041 caatgcagct gcgatgattc aacgtaaaga cgccatagta agccgcctga caatgggcat
    11101 cagcttgctg tttaagaaaa ataaagtgaa gcacctgtgc ggcctcgcaa cgttagaatg
    11161 tgctcaggat gagatttggc aattacgtgt aaacgatcaa caccttcacg cccgtaatgt
    11221 agtgattgca acaggttctc agccgcgtca gctgcctggc gttaccattg ataatcaaca
    11281 gatcctcgac aatcgtggcg ccctggcatt gagcgaagtg ccgccgcgtc tcggtgtgat
    11341 cggcgctggc gtgattggcc ttgaactggg gtctgtgtgg aatcgggttg gttcagacgt
    11401 cacgttgctg gagatggcgc caacgttctt gcctgcactg gaagctcgcc tgtcgaacga
    11461 agtccgtaaa gcaatgattg ccagtgggat gaaaatgcaa ctggcagtag agattgaggc
    11521 tattgaacaa cgggatgacg gtgtgcatgt gcgctggcgg cagggtgaga agcgagaaga
    11581 aagccgcttc gataaattaa ttcttgccat tggacgtgta ccgcgacttt ctggtgtcga
    11641 tctcgtgcaa ctcggtcttg aagccgataa ccgtggcggt attgccgtcg ataacttatg
    11701 ccgtaccggg aaagccgggc tgtgggcaat tggtgatgtc gtgcgagggc cgatgctggc
    11761 gcataaagcg atggctgaag gtgtagtggt ggctgatcaa attgccggac tggtggtaga
    11821 gccgattaat tttgcgctga tcccttcggt gatttacacc caaccggaag ttgcgtgggt
    11881 aggggaaaac gaggcctcac tgaaagccgc tggcagagtc tttaacaaag gaaattctct
    11941 gtttgccggt aacggtcgcg ccttagcatt aggccaggaa ggtgggcgtt gcacgttata
    12001 cagtgataaa cacactgacc gcgtacttgg cggagcgatt gttggcccgc aggcatcgga
    12061 gctgattaat gaaattgcgc tggcgatgac ttttagcgcc tctggcgagg atattgcctg
    12121 tgcgatccat gcgcatccga cattaagcga agtcatccac gaagcagcaa tggccttgaa
    12181 taataaagca ttacacggat aacacacacc actgagggaa tatatgaatt tacatgagta
    12241 tcaggccaaa tcgttactgg ctggcatggg gatgccgtgt ccaaaagaga tagctatcca
    12301 acagatttcg caactggcag atgcctggca acatattgct tgcccgagta aaggcgcggt
    12361 acttaaagcg caggtacatg ctggtgggcg cggtaaagct ggcggcgtaa aagtgcttaa
    12421 gcagctgccg gaagcacagg cttttgtaca gcaaatgttg ggatcgcaac tggtgactta
    12481 tcagaccggg ccagaaggac agtatgtcag cagtatttta ctgtgcgaaa acatttatcc
    12541 ggtacgccag gaactctatt ttggcatggt ggtggatcgt gaaagccagc gggtcacgtt
    12601 tattgtcagc ccggaaggtg gtgtggaaat tgaaaaagtc gcccatgaga caccggagaa
    12661 aatcagcagt gtcagcattg atccactgac aggcgtgcag ccttgccata tccgcgaaat
    12721 gtttgctgtt ctgcaactgg aacacggact atttgctgcc tttagtcgcc tggttaatca
    12781 ggcgtggaaa gcctttaacg aactggattt tgccctgctg gaaattaacc ctctggtgtt
    12841 gcgggaaacg ggcgaattta tgtgtgcaga cgcgaaagta tcgctggatg ataacgcgct
    12901 gtatcgtcac ccggaactcc aggtgctacg tgatgaaacc caggaagatc cacgcgaaag
    12961 ccaggcagcg aagcttgatc ttaattatgt ctcgctggac ggaaatatcg gctgcatggt
    13021 caatggtgca gggctggcta tggcaaccat ggacatcatc aagctatacg gtgaacaacc
    13081 cgcaaacttc ctggatgttg gcggtggcgc aacccaggag cgcgtcagtg aagcctttcg
    13141 gctgattgtt tccgacagca aagtgaaagc cattctggtt aatatttttg gtgggattgt
    13201 ccgttgcgac atgatcgcca gagccattat tcatgctctg aatgaggcca gcatcaccct
    13261 gcctgtcgtg gtgcgtctct ccggtaataa cgccgccgaa gggcagcggt tactggcaga
    13321 aagtggtctg acggtggaag ccgtcgattc tctggatgat gccgcgaaac gcatcattgc
    13381 gttactgaat taacaggagg ttaagatgag cgttttgatc aacaaacaca cgcgggtact
    13441 ggtgcagggc attaccggca aaaacggcac atttcataca gagcaggcta ttgcgtatgg
    13501 cacgcagatt gtgggtggag tgacgcctgg taagggcggg caacggcatc tggatcgccc
    13561 ggtttttgat tcaatgaaag atgcgatgcg acaaactgag gccgatgcca gcgtgattta
    13621 tgtgcctgca ccgtttgtgc tggattctgt tgttgaggct attgaagctg gcgtcaaatt
    13681 ggttgtggtg atcactgaag gtgtaccgac actggatatg gtgaaagttc gtcggctgct
    13741 tgactgccac ccggatgtgc ggctgattgg gccaaactgc ccaggagtta tcacgccggg
    13801 tgagtgcaaa atcgggatta tgcctggtga aatccaccgc ccagggcgta ttgggattgt
    13861 ttcccgctcc ggtacattaa cctacgaagc tgtagcgcaa accacggcgc tgggacttgg
    13921 gcaatccacc tgtattggta ttggcggcga tccggttccg gggactaact ttattgatgc
    13981 gctgcgttta ttcgaaaatg atccgcaaac agacgcggta attatgatcg gtgaaattgg
    14041 tggtaatgcg gaagaacggg ccgcggaatt tattcgccat gatatgaata aaccggtcgt
    14101 aggttatatt gccggtgtta ctgcaccaaa aggaaaacgc atgggacacg ccggagccat
    14161 tatttctggc ggtagcggca gtgcagagga taaattccgt gcgttcgata aagcaggcat
    14221 ggcatggaca agaaacccgg cgttgcttgg cgaaacgtta tggaatgtta ttaagtaatc
    14281 cataaatgaa tatcaaggga gcatatcgct cccttttttt atctgtgtgg taagagaatc
    14341 accttttatt tactgtg
//
LOCUS       DACADF010000118        14214 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.120, whole genome shotgun sequence.
ACCESSION   DACADF010000118 DACADF010000000
VERSION     DACADF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.120"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..98)
                     /locus_tag="ISB30_003483"
     CDS             complement(<1..98)
                     /locus_tag="ISB30_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP1991083.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNT"
     gene            244..423
                     /locus_tag="ISB30_003484"
     CDS             244..423
                     /locus_tag="ISB30_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1991084.1"
                     /translation="MRPSKRTVRFETQPGYHLSATPGKKLIWWLPDNRALGLTAASLN
                     HAEYQRRALPVKRET"
     gene            1623..2768
                     /locus_tag="ISB30_003485"
     CDS             1623..2768
                     /locus_tag="ISB30_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991085.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTKWLKKEPRASYF
                     FLFRLYTRIHNTFFPQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPHPIPEMD
                     SSIVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAM
                     GFPSQPKIAGPREKPMRTVHPDNIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQASFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYQDFVANEQHKTLS"
     gene            complement(3299..3556)
                     /locus_tag="ISB30_003486"
     CDS             complement(3299..3556)
                     /locus_tag="ISB30_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991086.1"
                     /translation="MQHIDRLNVIKALVLLEDEQIVRFNIAANDNASQIHMLVDGLGV
                     GLTHEPVAAGMISHRTACHRSRQHDRPAHSYFYYLSAYQVA"
     gene            complement(3610..4377)
                     /locus_tag="ISB30_003487"
     CDS             complement(3610..4377)
                     /locus_tag="ISB30_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1991087.1"
                     /translation="MSGLTINALCAGYGKRRIIEHLSISTLPRGEVTVLLGPNGCGKS
                     TLLRALAGLNRASGEAWLNEENLLSLPFARRAEKVVFLPQSLPQGVHLQVLESVVVAQ
                     RASGAGQNQAQAIALLEELGIAHLAMNYLDSLSGGQKQLVGLAQSLIRRPELLLLDEP
                     LSALDLNYQFHVMDVVSRETRRRNMVTLVVLHDINIALRHAAQVIMLKEGKLIDSGDP
                     QTVIHAESLAQVYGVRGRVERCAQGRSMVIVDGAIEK"
     gene            complement(4374..5432)
                     /locus_tag="ISB30_003488"
     CDS             complement(4374..5432)
                     /locus_tag="ISB30_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP1991088.1"
                     /translation="MTVLESTLSVENRYRQLFRQRLVILAVIFIAIIASLLLDFTLGP
                     SGLPLHSLIKTLMHPSGATNGMRVIVWDIRLPYALMALLVGMALGLAGAEMQTILNNS
                     LASPFTLGVSSAAAFGAALAIVLGIGIPGVPESGFIPANAFLFALLSALLLDSLTRWT
                     RVPTSGVVLFGIALVFTFNALVSIMQFVADEDTLQGLVFWTMGSLARASWEKLAVLAA
                     AMAIVLPWSLRRAWQLTALRLGEERAMSFGIDVRRLRLGSLLRISLLAALSVAFVGPI
                     GFIGLVAPHISRLLLGEDHRFYLPGSILIGGLVLSLASVASKNIIPGVILPVGIVTSL
                     VGVPFFLSIVMRHRGSMS"
     gene            complement(5451..6440)
                     /locus_tag="ISB30_003489"
     CDS             complement(5451..6440)
                     /locus_tag="ISB30_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP1991089.1"
                     /translation="MKLSRLFPLLALLLAASLHAENTHKEVRIASPWPAQNTIIAMLG
                     YGDNIVGTSMIAKRIPLFRQSLPRIEKVAAVSVNSGHEINPEQIIALGVDMLFVPQNM
                     VVPQQALLKQAGVQVLAFEANSLRALTQRVQQTAAVLGPDAQQKALAYQRYFDRNVAL
                     VTGRLKDLPASQRVSLYHSMGNPLTTTGRPSLNQDWIDLAGGKNIAENWFGEHQQNRS
                     GEVALEKIVTANPAVIIAMNKRDADAILSSPQWASVDAVIHHRVYVNPKGMFWWCRET
                     SEEALQFLWLAKTLYPARFADVDIRKETREFYRQFFGLTLSDAQMSDVLNPPR"
     gene            complement(6451..8616)
                     /locus_tag="ISB30_003490"
     CDS             complement(6451..8616)
                     /locus_tag="ISB30_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAP1991090.1"
                     /translation="MKTQITFAALLPALASFIPLHAHASSTSEDEMIVTGNTAADTTD
                     SAAGAGFKTNDIDVGPLGTKSWIETPYSSTTVTKEMIENQQAQSVSEMLKYSPSTQMQ
                     ARGGMDVGRPQSRGMQGSVVANSRLDGLNIVSTTAFPVEMLERMDVLNSLTGALYGPA
                     SPAGQFNFVAKRPTEETLRKVTLGYQSRSAFTGHADLGGHFDENKRFGYRVNLLDQEG
                     EGNVHDSTLRRKLVSVALDWNIQPGTQLQLDASHYEFIQKGYVGSFNYGPNVKLPSAP
                     NPKDKNLALSTAGNDLTTDTISTRLIHYFNDDWSMNAGVGWQQADRAMRSVSSKILNN
                     QGDISRSMKDSTAAGRFRVLSNTAGLNGHIDTGSICHDLSLSTTGYVWSLYSAKGTGS
                     SYSWGTTNMYHPDAIDEQGDGKIRTGGPRYRSSVNTQQSVTLGDTVTFTPQWSAMFYL
                     SQSWLQTKNYDKHGNQTNQVDENGLSPNAALMYKITPNTMAYVSYADSLEQGGTAPTD
                     ESVKNAGQTLNPYRSKQYEVGLKSDIGEMNLGAALFRLERPFAYLDTDNVYKEQGNQV
                     NNGLELTAAGNVWQGLNIYSGVTFLDPKLKDTANASTSNKQVVGVPKVQANLLAEYSL
                     PSIPEWVYSANVHYTGKRAANDTNTSYASSYTTWDLGTRYTTKVSNVPTTFRVVVNNV
                     FDKHYWASIFPSGTDGDNGSPSAFIGGGREVRASVTFDF"
     gene            9045..9479
                     /locus_tag="ISB30_003491"
     CDS             9045..9479
                     /locus_tag="ISB30_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991091.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLTRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            9697..12081
                     /locus_tag="ISB30_003492"
     CDS             9697..12081
                     /locus_tag="ISB30_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAP1991092.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNEKRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDECQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSVGRLFVLVNKFDEKDRNGDGADAVCQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKDYVCKDSELATEGATDL
                     WEGSLIDQLITEVILSSHSRAAALAVDSAAAKLMQNAENISEYLSLRHQGLMQSIQSL
                     QAHITSLLEDIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFLDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINTRLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRRRQSGLWGKICGAFGTSDWGW
                     ETYKEDVSRSVININTVRKEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTRRLQALNERVPELITDSKALREELETML"
     gene            12078..12983
                     /locus_tag="ISB30_003493"
     CDS             12078..12983
                     /locus_tag="ISB30_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAP1991093.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGLEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAG"
     gene            12980..14050
                     /locus_tag="ISB30_003494"
     CDS             12980..14050
                     /locus_tag="ISB30_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP1991094.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPVTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
BASE COUNT         3595 a         3669 c         3724 g         3226 t
ORIGIN      
        1 gtattgcggg agatccccag ttctctggca atcgcccggc tactcattcc ctgcttgtgc
       61 aggattttaa tttccataac tgtctcaaaa gtgaccataa gctctcctga atcaggagag
      121 cagattaccc cctggatctg atttcaggcg ttgggtgtgg atcactattg caccgttcgt
      181 gacacaatgg gccatacggg ccgacgttcc atgccgcgtt actacattca gcccggacgt
      241 aaaatgcgcc cgtcaaaaag aacggttcgc ttcgaaactc agccaggata ccacctctcc
      301 gccacacccg ggaagaagtt gatatggtgg ttgccggaca acagagctct tggattgact
      361 gctgcatcac tcaaccacgc tgaatatcaa aggagagcgt taccggttaa aagagaaacg
      421 taaagctgtg gtgatgaccc aaaacgcaac gcctgtcagt gaggatgaaa tggtgaaaag
      481 cggacagcat cagtaacgga agtatcttag cgggtacgaa aatggcagga tcgtgacatt
      541 gacagaatat ctcctgatca tcctgaatga ctgcagttgc tgtggaaaag taagctgtct
      601 ccacccacgg cacgacttaa caggcattcc gtaactcccc cagacttact atgacgcatt
      661 atcggtgcac ttaaaaccgc agaccgcatt gcgggtgtta ctgcatgaca gtctgaccgt
      721 attgtgagat gtcggcagga accgatgtcc gcgcaggggg gaacttcctg cagtacctga
      781 cgcttcaaat agtattaacc gtagggcgtt gttggttccg gatcgcggaa atgggagcac
      841 aggtctgcta cggggtgtac ctctgacagt aaatccggct cgctcatctg acagcctctc
      901 tcccggaaaa gaccaaaaat gagatcgtct cctcaacagc agagaagagg tcttgacaga
      961 caggctacat tgcatacgat ttatgaagaa aaagacagca aatatggagg ttatagcgga
     1021 gaacacccta cgaaattcct atgaaaaacg attgaaaaaa atatcaaatt cgattcgttt
     1081 ttatatgctt tttgggaacg tggcgatgac gaactgttgc atgtgcagag gagaatgcac
     1141 ccggaagcac cagaatcatc cagaaaggca gaacgattac gcatcaaacc aggagacagc
     1201 aatatcaagg taatacactg atttaaaatg aatttacagc aatacgtgaa aacatttttt
     1261 acttaaagga gacagatgcg ctgaagagag tgcaggtatt tttatataaa aatgagaaga
     1321 ttacgggata tatatccggc acactatgac cggagaatac cagtcaggct taaaaagaca
     1381 ataaatacag tgagttaaat acacagagaa aacgaaaacg tagtatcaga gggtacatct
     1441 tacaacagag actgaatttc cggtttttca taaccggcgc attccggaaa atgcaggcgg
     1501 cccggagctc ttctgacgcc agtacctgat agattgttct gtacattctg tacatcatgt
     1561 acggaggttt ttgtttgctt tcttaaaatc tgcaaatgtg ggaagagcag gtgaggtgta
     1621 ttgtgggaaa ggaactggat gatatctgca ccagctgtcc gtatatcgat gctgtaaaac
     1681 ggcacaaaca acagctcggt gccatagagg aatatacaaa gtggttaaaa aaagaaccac
     1741 gggcatctta tttttttctg ttccggctgt atacccgcat tcacaataca tttttccccc
     1801 aaaagcaaca actcccgttc actcccggtg gcactcactg cccggaacct gatgtcaccc
     1861 ttcgggactt aacgctttcc ccgggctacc acagtgatta tgcccctcac cccattccgg
     1921 agatggacag ttctattgtt gttcccccca ctaatgaaaa cacctcgcct ccggaagata
     1981 ctcccgacaa cactccggcc ggaggcaata ccggacaagc ggaaaagacc agaaacgcag
     2041 gactcacacc cattcctgaa aaacgttcag gaatgccccc ggaacatctg cggtttgcca
     2101 tgggttttcc atcgcaaccg aagattgccg gtcccagaga aaagccgatg cgcaccgtgc
     2161 atccggataa tatctaccgt gaaattatat ggttctgctc cagttacctg ctgaaatccg
     2221 gaccagaggc tacgaggacg attatcaact cagtattctc tgaatgggcc agcataaaca
     2281 atgattaccc ctcccccttt tcgtgggtgg acagcaggga cagtgaacag tgtgactggt
     2341 tatggaacgc catgcaggtc cggtgtgtgg gaaccccgct gaatcccctt accccggagc
     2401 agaaatactg gttcgcctgc gccacgtttg ataactggga aggctggaat gagcaacagg
     2461 tacagttttt actggaaagt aatcccagac ggaacagagc gaagtttacg caggcctcct
     2521 tccaggcccc caggattcag cataaagcga ttcttcttga tgagctgaaa agtgcccggg
     2581 agcaacaaaa aaggcgtgat gaacgtgctg atggttccgt cccgctgaaa ctgtccggaa
     2641 aaatccacaa acagcttgaa agtattgccc gaagtcgtgg tgtcctccca aaaaaactgc
     2701 tgaatgaaat gattgagcag gcgtaccagg actttgtggc aaatgagcaa cacaagacac
     2761 tgagttaagg accgcagggc ctccggttac cgattctgca gcatacacaa cgaatgtgtc
     2821 cggctcttac ctgtgggggc cgcactaaag ttacgcattg agtatgagtg ggtaatcccg
     2881 gatgagaata tcccagtttt tacactattc actgaaggtg cgacatatca ttcagtctct
     2941 ctgaattctt catttcagtg gccccggcta cagtcgttta atctcctcca cgaaccgggg
     3001 ccgcaacttg ttgtgtcaat accaaattgt tggtcattac tgtcttgtgt cactggcgct
     3061 gacaagtgaa ctgaaatccg caccttaata ttctgactgg ctgcgtacat tcatggcatg
     3121 aagcggccgg agcggctgaa ctgaaggtcc gttgtgagcg aacagcggac attcactaca
     3181 catggttcaa acatgaaccg gagctttatt gtctagctgt cagtgtactg cgatttaccc
     3241 gctcggctgt ccagaaaatc ggtctgaaat ccagtcatta aagggcctta gcctgacatt
     3301 aagcaacttg gtaggcggat agataataga aataggaatg cgcaggtctg tcatgttgcc
     3361 tagaacggtg acaagctgtc cggtgcgaaa tcattccggc agcgacaggc tcatgtgtca
     3421 gcccgacgcc cagaccgtca acgagcatgt ggatctgcga ggcgttatcg ttcgcagcga
     3481 tgttaaaacg aacaatctgc tcatcttcta gaagcacaag tgctttaatg acattaaggc
     3541 ggtctatatg ttgcattaca gacttcctat tgatctttac ctgaggctga agcaaccacg
     3601 ttaatccgac tatttttcga ttgcaccatc cactatcacc atcgatcttc cctgagcaca
     3661 acgttcaact ctcccgcgta cgccgtatac ctgcgcaagg ctctctgcat ggatcaccgt
     3721 ttgcgggtcg ccgctgtcga taagtttccc ctctttcagc atgatgacct gggcggcatg
     3781 ccgcagtgcg atattgatat cgtgtaagac gaccagcgta accatattgc gtcttcgcgt
     3841 ttcgcgcgat acaacatcca tcacatggaa ctgataattg agatccagcg cgctcagtgg
     3901 ttcatccagc aataataatt cggggcggcg aataagcgac tgtgcaagcc ccaccagctg
     3961 cttctgaccg ccggacaggc tatcgaggta gttcattgcc agatgtgcga tgcctagctc
     4021 ttcgagcagc gctatagcct gcgcctgatt ttgcccggcg ccggaggcgc gctgggcgac
     4081 aaccaccgac tccagcacct gtaaatgcac gccctgcggc agggactgcg gcaggaacac
     4141 aactttttca gcccgacgag caaacggcag cgataagaga ttctcttcat tcagccaggc
     4201 ttcgccactg gcgcgattta gcccggccag ggcgcgcagt agcgttgatt taccgcagcc
     4261 attaggcccc agcagtacag tgacttcgcc gcgcggcagc gtagaaatcg acagatgctc
     4321 aataatccgc cgtttgccgt aaccggcgca cagcgcatta atcgtaagcc cgctcatgac
     4381 atactccctc gatgacgcat tacaatgctc agaaagaacg gaacgcctac cagcgaggta
     4441 acaattccta ccggtaaaat gacaccggga atgatgttct tcgacgctac cgaggccagc
     4501 gatagcacca gcccaccgat aaggatgctt ccgggcaaat aaaagcgatg atcttctccc
     4561 agcaacaggc gggaaatatg cggtgcaacc aggccaataa agccaattgg ccccacaaac
     4621 gcaaccgaaa gggccgccag caggctgatg cgcagcaggg aacccaggcg caaacggcga
     4681 acatcaatgc caaaactcat cgcccgctct tcgccgaggc gtagcgcggt aagctgccag
     4741 gcacgacgca gcgaccaggg taaaacaatc gccatcgccg ccgccagcac cgccagtttc
     4801 tcccatgatg cgcgagccag gctgcccatc gtccagaaca ccaggccctg caaagtatct
     4861 tcatcggcga caaactgcat aatggacacc agcgcattga aggtaaacac cagcgcaatc
     4921 ccaaacagca caacgcccga cgtcggcacg cgcgtccagc gggtcagact atcgagcagc
     4981 agggcggaca ggagcgcaaa aagaaaagcg tttgccggta taaacccgct ttcgggaacg
     5041 ccgggaatac cgatgcccag aacaatcgcc agcgctgcgc caaaggccgc cgccgacgaa
     5101 acgccaagcg taaacgggct ggcgagggag ttattcagaa tagtttgcat ctctgccccg
     5161 gccaaaccca gcgccattcc taccaggagc gccatcagcg catagggcaa acggatgtcc
     5221 caaacgataa cccgcatgcc gttagtcgcc ccggacggat gcatcaaagt ctttatcagc
     5281 gaatgtaaag ggagccctga ggggccgagg gtgaaatcga gcagcagcga agcaatgata
     5341 gcgataaaaa taaccgccag tattaccaga cgctggcgaa aaagctggcg atagcggttc
     5401 tcgaccgaca gcgtactttc caatacagtc atggtttttc acagcctccg ttagcgcggc
     5461 ggattgagca catcgctcat ctgcgcatcg cttaacgtca ggccaaagaa ctgccgataa
     5521 aactcacgcg tctctttgcg tatatccaca tcagcaaatc gcgcaggata aagcgttttc
     5581 gccagccaca aaaattgcag cgcctcttcg ctggtttcac ggcaccacca gaacattcct
     5641 ttcggattaa cgtaaacgcg atgatgaatg accgcgtcca cgcttgccca ctgcggagaa
     5701 ctcagaatgg catccgcgtc gcgcttgttc atcgcgatga taaccgccgg attggcagtg
     5761 acaattttct ccagcgcgac ttcacccgaa cggttttgtt gatgctcgcc aaaccaattt
     5821 tcagcgatat ttttgccgcc ggccaggtca atccagtcct ggtttagcga gggtctaccg
     5881 gtggtggtca gcggattgcc catactgtga tagagcgaca cgcgctgtga cgcaggaaga
     5941 tctttcaggc gcccggtgac cagggccacg ttgcgatcga agtagcgttg ataggcgagc
     6001 gctttttgct gcgcatcggg ccccagcacc gctgccgttt gctgtacgcg ctgcgttaac
     6061 gcccgcagtg aattcgcctc aaacgccagc acctgcaccc cagcctgttt cagcaacgcc
     6121 tgttgaggca ctaccatatt ctgcggtaca aaaagcatat cgacccccag cgcgataatc
     6181 tgttcgggat tgatttcatg cccgctgttt acgctaactg ctgcgacttt ctctatgcgc
     6241 ggcaggctct ggcgaaacag ggggatcctt tttgcaatca tcgaggttcc gacaatgtta
     6301 tcgccgtacc ccagcatggc aataatggta ttttgcgccg gccacggtga cgcgatacgc
     6361 acctccttgt gcgtattttc tgcgtgcagg ctggccgcca gcagcagcgc cagcagcgga
     6421 aataaacggg agagtttcat catttatcca tcagaaatcg aaggtgacgg atgcacgcac
     6481 ttcgcggccg ccgccgataa acgcacttgg ggaaccgtta tcgccatcgg tacccgatgg
     6541 gaagatagaa gcccagtaat gtttatcaaa cacgttgttt accaccacgc ggaaagtggt
     6601 tgggacgttg ctcactttcg tggtgtaacg cgttcccaaa tcccatgtgg tatagctgct
     6661 ggcgtaagag gtgttggtat cgttcgccgc gcgtttgccc gtataatgga cgttagcgct
     6721 gtaaacccat tccggtatgg acggcaaact gtattccgcc aacagattgg cctgcacttt
     6781 cggcacgccg acaacctgtt tattgctggt tgaggcattc gccgtatctt tcagtttcgg
     6841 gtcgaggaag gtcacgccgc tgtaaatatt cagcccctgc cacacattcc cggcagcggt
     6901 taactcaagg ccgttgttaa cctggttacc ctgctcttta tacacgttat ccgtatcaag
     6961 ataggcaaac ggacgttcca gtcggaacag cgcggcgcct agattcatct cgccgatgtc
     7021 cgattttagc cccacttcat actgcttgct gcgatacggg tttagcgttt gaccggcatt
     7081 ttttacgctc tcatccgtcg gtgcggtacc gccctgctcc agcgaatcgg catagctaac
     7141 gtaggccatt gtgttagggg taattttata catcagcgcg gcgttcggac ttaaaccatt
     7201 ttcatcaacc tggttcgttt gattaccgtg cttatcgtag tttttagtct gcagccagct
     7261 ctggctgaga tagaacattg ccgaccactg cggcgtaaat gtcaccgtat cgccgagcgt
     7321 aacgctctgc tgagtattta cgctggagcg gtatcgcggc ccgccggtgc ggattttgcc
     7381 atcgccctgc tcatctatcg catccgggtg atacatattt gttgtacccc agctatagct
     7441 ggaacctgtt cctttggcac tataaagcga ccagacatat cccgtggtag aaagtgacag
     7501 atcgtggcag atagagccgg tatcaatatg accattcagc ccggcggtgt tgctcaggac
     7561 gcgaaaacgt ccggcagcgg tggaatcctt catcgaacga gagatatcgc cctgattgtt
     7621 gagtatttta ctggaaacac tacgcatcgc gcggtcagcc tgctgccagc ccacgccagc
     7681 gttcatggac cagtcgtcgt taaagtagtg gatcaggcga gtgctgatgg tatcggtagt
     7741 gaggtcgttg cccgcagtgc tgagcgccag atttttgtcc ttcggattcg gcgcagacgg
     7801 cagtttgacg ttcggcccat agttaaagct accgacatag cctttctgga taaattcgta
     7861 atggctggcg tcgagctgta gctgagtgcc cggctgaata ttccagtcga gcgcaacgga
     7921 aacgagtttg cgacgcagcg tgctgtcatg cacattgcct tccccttcct gatcaagcag
     7981 gttcacgcga tagccaaacc gtttgttttc atcaaaatgg ccacccagat cggcatggcc
     8041 ggtaaacgca ctgcggcttt gatagcccag cgtcacttta cgcagcgtct cttcggttgg
     8101 gcgcttcgcc acgaaattaa actgccctgc tgggctcgcc gggccgtaca gcgcgccggt
     8161 caaactgtta agcacatcca tgcgctcaag catttccacc ggaaacgcgg ttgttgaaac
     8221 gatattcagc ccgtccagac ggctgttggc caccacgctg ccctgcatcc cccgactttg
     8281 cggacgcccg acatccattc caccgcgcgc ctgcatttgc gtactgggag agtatttcag
     8341 catctcgctg acgctttgcg cctgctgatt ttcaatcatc tctttagtaa cagtggtgct
     8401 ggaatatggt gtttcgatcc aggatttcgt tcccagcggg ccgacatcta tatcgttcgt
     8461 tttgaaaccg gcaccggcgg cagaatcggt ggtgtcggcg gcggtgttgc ccgtgacaat
     8521 catttcatct tcagaagtag acgaggcatg agcatgaagc ggtatgaaag acgctaatgc
     8581 tggcaaaagc gcagcgaaag ttatttgagt tttcatgaat cactgatcgt tatgtaaata
     8641 tttatattac gatcggtgca taggcaaaat tatgatccag atcacacgtg aatgtaagtt
     8701 atttacaaat tacgctaacc ttctgtctcc gctctttgca gcataaaagt atggcggcgc
     8761 gttgttgaaa gcaggagcgg ccgggttgtc agaaagctgg tttggacgca agtaatatat
     8821 aacaactctg attaccgcat actgtcagga cagtatctac ttgagcgtcc gcctttggca
     8881 caaagcggac tgtcagatta gattcagacc tgtactcatt agctgataca aatcagccac
     8941 atatacctgc attcaaaata atttcagaaa gatattatct ccgtgaatcc cccgccacct
     9001 ttccggtgca cggggttttg tcttttttca ctgggaatac atgtatgaat ccgtctgatg
     9061 ccattgaggc aattgaaaaa ccgctctcct ccctgcctta cccgatttcc cgtcacatcc
     9121 tggaacatct gcgcaaactc acccgtcacg aacccgtgcc tggcattatg ggtaaaagcg
     9181 gggccggtaa atcctcactc tgtaatgcac tgtttcaggg ggaggtcacc ccggtcagtg
     9241 cactcatgac agtgcttccc ggacatgccg ccatccctct gatgacacgc ctgcaggacg
     9301 aactgcgcac ggagtctgtc cgcactcaga cccgtgaaca gtttaccggt gcggtggacc
     9361 ggatatttga cacggcggag agtgtctgca ttgcctctgt tgcacgcacg gttctgcgcg
     9421 ccgtccgtga ctcggtggtc tctgttgccc gcgcggtatg gaactggatt ttcttctgaa
     9481 cccggtggtg atgtaccagg ctgttgttac agatttttaa ctccatgaaa acactatgca
     9541 aacgcagcca tacccggggt gtccccttgc gttgtgtttt tcaagttaat agaatacatc
     9601 gatcgttact atcatatcga tcgttataaa cgatcgtttg tggtaaatga cctcctccgt
     9661 ggttgtcatg aaaatgcgtc tcaggaaggt taaataatgc acgaaaagaa catcgccctg
     9721 ctttgtgatg aagccgaccg acttttgcaa ctgaacatta atctgctccg gcaaatggtt
     9781 gaggagccag atgtgttatc tgacagtaag aacgaaaaaa gactgctttt tgataaacag
     9841 aaagcactga aaagaattga ggagctggag ggcgaacaaa tcaaaaccgc ccgcagggag
     9901 atggtgctgg ctgttgtcgg cacgatgaaa gcaggtaaat caaccaccat caacgccatt
     9961 gtggggcagg aaattctgcc taaccgtaac cgccccatga cctctgtacc gacgctcatc
    10021 cgccacgttc ccggaaaaac tgagccggtt ctccatctgg aacatattca gcctgtccgc
    10081 aatttattaa tcacactgca ggaaaaactc gccaccccgg caggacagca ggtcgcacag
    10141 accctgcagc aaaccgggga tacccgcgaa ctgctggata ttctgacgga tgatggctgg
    10201 ctcaaaaatg aataccacgg ggaggaggaa atctttaccg gactggcatc gttaaacgat
    10261 ctggttcgtc ttgctgcggc aatggggagt gaatttcctt ttgatgaata cgcagaagtg
    10321 cagaaactgc cggtgatcga cgtggaattc agccatctgg tggggatgga tgaatgccag
    10381 ggaacactca cactgctgga tacccccggc cctaatgaag ccggacaacc gcagatggaa
    10441 gtgatgatgc gggatcaact gcagaaagcc tctgcggtgc tggcggtgat ggactacacc
    10501 cagatgaact cgaaagccga tgaggaggtt cgtaaagagc ttaatgccat tgctgatgtg
    10561 tctgtcggcc gcctgtttgt gctggtcaat aaatttgatg aaaaagaccg taatggcgat
    10621 ggggctgatg cagtctgtca gaaggtaccg gcaatgctga acagcgatgt gctgcccgcc
    10681 tcccgcgttt atcccggatc ctcacgccag gcatacctgg ctaaccgtgc gcttcatgag
    10741 ttacggaaaa acggaaccct tcctgttgat gaagcctggg tcgatgattt tgtcagggaa
    10801 gccttcggtc gcatgaaaaa agattacgtc tgtaaagaca gcgaactggc aactgaaggg
    10861 gcgacagacc tgtgggaagg ctcacttatc gatcaactga taacggaagt catactgagt
    10921 tcacactcca gagctgcggc actggcggtt gattctgccg ccgcaaaact gatgcagaat
    10981 gcagaaaata tcagtgaata cctgtcgtta cgccatcagg ggctgatgca aagtattcag
    11041 tcactgcagg cgcatatcac cagcctgctt gaggatatcc gggaaatagc agactgtcag
    11101 gagcaggtga ccgctgatgt cagaatggcc atggaggaga tcgatgccag aacccgggaa
    11161 ttactgacgg gggtctgcac ctcactggaa gaggagctga atgactattt cagaagcggt
    11221 aaacgcaaag aacagcaaat gctggaggaa gaaaattcag cgcagccccg ggagagaaac
    11281 gcattcgctt tctttctcga tattttcgga accggaaatc agcatgacag gatgcgggac
    11341 tttgatcccg acagccccga aataaaattc agtgatcgca gggaagccct tgaactgatg
    11401 acgcaaatcg aatcgaccgt gaccagcctg caccgtgagg ctgaagcaca gttccggcct
    11461 gagctggaga aaatcgtcag cgggattgaa acaggttttc gtggcacggc cctgtacgcc
    11521 acagaaaaca ttgccggtcg catcaatacc cgcctggagg atgagggctt taccgtaaaa
    11581 atcagttttc cggcagtcag tcagttacaa acccggctcg cggtaaaaac aaatctgagt
    11641 gcgcttatgg aggaaagaac ggagaccgtc acccgtcgcc gtcggcagag tggtttatgg
    11701 ggaaagattt gtggagcgtt tggcaccagt gactggggct gggaaaccta taaagaggat
    11761 gtgagccgca gtgttatcaa tatcaacacc gtcaggaagg aagttatgtc actgacccgg
    11821 gcatatttcg gggagctgca ggcatccatt gagcaggata ttaaccagcc cgtccgccag
    11881 gaaatcgatg cctttttctg cgcattcagg gagaaagttg aacaactgcg taacacgctg
    11941 attcagagct ctgaagatca taaacgcgat cagcaggcac aggaacggct taccaggaga
    12001 cttcaggcat taaatgaaag ggttcctgag ctcattactg acagtaaggc gctgagggaa
    12061 gagctggaga caatgctgtg acttcaccat ttattcaaca aattgctgat aaccgggtat
    12121 gtcaggtact cacctgtctt cctgaaaaat ttgtggttga tttcgcaaac ggaatcgatg
    12181 ttgcacagga gcatatccgc acggccggag agcgcacgtt cttccggcgt ttaaaagagg
    12241 gactgaccgg cgaaggcgct gcacggcaga atgccatcaa tgcttcggtt gcacagggcc
    12301 ttgaggcgtc cctgcgctgg ctgacggagc tgacaacgtc acttgccacc acaaactacg
    12361 cgattacccg ggtaaacgac agggtcagtt cactggtcag tgatactgcc aggctggcgc
    12421 attattccgc agatacacgg gagcagttac tcatcctggc cgatcaggtt caccataaac
    12481 tgaatcatct tgaagaaaaa ctccaccgtg ttgaccaggt tcagcgggcg cagttacatc
    12541 ttgagcagat attctcatgg tggagcgccg ggcgatatgc gtcattttcc cctgccggac
    12601 gttgttatgt ggcgcttgaa gagcttcgct ggggcgcgtt tggtgatgtg atacgtcagg
    12661 gcgaaacagg ccaggttaac cagctactgg atattctcag acataaagca ttaacgcaga
    12721 tggcacagga gagtggcggt agtgcaaccg tgcgtctgaa cactctggac tggcttggtg
    12781 gtcagggccg ggagcaggca gataacgagt ggcatgacgc gattaactgg ctgggagact
    12841 ggtgcagtga agagcagcat ccggtgatct ggtcaaccac gcaggctgca gaacacttac
    12901 cggttcgcat gcctcgcctc tgctctgcag aacgcctctc tgaaagtatg gtcgatgaaa
    12961 tatttcagaa gggggctgga tgagtacaga aatgaaaacg gggctggtgc tgtccggtgg
    13021 cggggcggtg ggcgcttatc aggcgggagt ggttaaggca ctggcagagt gtggtacaca
    13081 gatcagcatg gtttcagggg ccagcattgg cgcattcaat ggtgccatta tcgcggcctc
    13141 tcccgatctg tcagaagctg ccgtacgcct ggaggcgctc tgggatcatc tggggaataa
    13201 tcaggtgctg tcggtaaacc gattggttta cttttcattg ctgaaaaaat tgttccaggc
    13261 aatgaacctc tgccagatcc ccggacgtgc aggagcactg cttacgacgc ttcttcgcca
    13321 tatatcgata ctcaacgggt ttgacaatct gatggctcag ccgttgttgt cagatgagcc
    13381 cctgacagcg ctgttggatc attatcttga tactgatgct ctggcagacg ggctaccgct
    13441 gtatgtgtcg ctgtacccca cagaaggggg catgcaggat attattgact gcattcgtgc
    13501 tgaactgggt gccggaacca cgaaaaacgc cgtttttcag catatccaga gcctgccccg
    13561 cggacagcag aaagaggctc tgcttgcgtc agccgcgctg cccctgctgt tccgtccccg
    13621 tgaggttcag gggacaatgt tcggtgatgg tggtatggga ggatggcgaa atatgcaggg
    13681 aaatacccct gtgacgcctc tggtcgatgc cggatgcaat atggtgattg tgacgcatct
    13741 gagtgacggt tctttatggg atcgccaggc ttttccggtc accacaatcc ttgagatccg
    13801 tccccggaaa aggctgaaat atgcaggtga tggtgacaac agcggcggtc tgctcagttt
    13861 cacgttggca cataccgacg cctggcgtca gcagggctat gaagacacga tgctgacgat
    13921 ggagcatatc cggaaaccgc tggcagcacg tcaggtactg tcccggtcag aaacggtgtt
    13981 gcagaaaagt ctggagataa cggaagaggc agatttggca ctgagaaacg cgatggcccg
    14041 gattaaataa agacgcccca aagaaacgcc accgcctgtg gtggtgtttt tcctggctgg
    14101 tgttattgaa aatattttca gatatatatt acctgagaga aagttttacc cctcacctgt
    14161 caattcactc actggaaatt taattatgca attacctgta aagttattaa tgag
//
LOCUS       DACADF010000119        14211 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.121, whole genome shotgun sequence.
ACCESSION   DACADF010000119 DACADF010000000
VERSION     DACADF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.121"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(184..936)
                     /gene="yecC"
                     /locus_tag="ISB30_003495"
     CDS             complement(184..936)
                     /gene="yecC"
                     /locus_tag="ISB30_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAP1991095.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(933..1601)
                     /gene="tcyL"
                     /locus_tag="ISB30_003496"
     CDS             complement(933..1601)
                     /gene="tcyL"
                     /locus_tag="ISB30_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAP1991096.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1616..2602)
                     /gene="dcyD"
                     /locus_tag="ISB30_003497"
     CDS             complement(1616..2602)
                     /gene="dcyD"
                     /locus_tag="ISB30_003497"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAP1991097.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEVAVNISSVVVASGSAGTHAGLAVGLEQLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2707..3507)
                     /gene="tcyJ"
                     /locus_tag="ISB30_003498"
     CDS             complement(2707..3507)
                     /gene="tcyJ"
                     /locus_tag="ISB30_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1991098.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3595..4146)
                     /gene="fliZ"
                     /locus_tag="ISB30_003499"
     CDS             complement(3595..4146)
                     /gene="fliZ"
                     /locus_tag="ISB30_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAP1991099.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4192..4911)
                     /locus_tag="ISB30_003500"
     CDS             complement(4192..4911)
                     /locus_tag="ISB30_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAP1991100.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5075..6124)
                     /locus_tag="ISB30_003501"
     CDS             complement(5075..6124)
                     /locus_tag="ISB30_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079755.1"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAP1991101.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTAEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKNDSMKIQIGANDNQTISIGL
                     QQIDSTTLNLKGFTVSGMADFSAAKLTAADGTAIAAADVKDAGGKQVNLLSYTDTASN
                     STKYAVVDSATGKYMEATVVITGTAAAVTVGAAEVAGAATADPLKALDAAIAKVDKFR
                     SSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLS
                     KANQVPQQVLSLLQG"
     gene            6372..7787
                     /gene="fliD"
                     /locus_tag="ISB30_003502"
     CDS             6372..7787
                     /gene="fliD"
                     /locus_tag="ISB30_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAP1991102.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKNSQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAGNSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGSNNAMKLSVSGDSALESFMGYNGTPGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDSLEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKNDAAGIGEMFIGDGKSTGVTTGI
                     SSNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            7803..8213
                     /gene="fliS"
                     /locus_tag="ISB30_003503"
     CDS             7803..8213
                     /gene="fliS"
                     /locus_tag="ISB30_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAP1991103.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8213..8578
                     /gene="fliT"
                     /locus_tag="ISB30_003504"
     CDS             8213..8578
                     /gene="fliT"
                     /locus_tag="ISB30_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAP1991104.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKIKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            8656..10143
                     /gene="amyA"
                     /locus_tag="ISB30_003505"
     CDS             8656..10143
                     /gene="amyA"
                     /locus_tag="ISB30_003505"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAP1991105.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10177..10590)
                     /locus_tag="ISB30_003506"
     CDS             complement(10177..10590)
                     /locus_tag="ISB30_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP1991106.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLTGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            10777..11982
                     /gene="yedE"
                     /locus_tag="ISB30_003507"
     CDS             10777..11982
                     /gene="yedE"
                     /locus_tag="ISB30_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAP1991107.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            11979..12212
                     /gene="yedF"
                     /locus_tag="ISB30_003508"
     CDS             11979..12212
                     /gene="yedF"
                     /locus_tag="ISB30_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAP1991108.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12321..12818
                     /locus_tag="ISB30_003509"
     CDS             12321..12818
                     /locus_tag="ISB30_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991109.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSHMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQAVFIAAIGSTPFERGDEAGRSFCKPAQGLSVTVAMA
                     VRANL"
     gene            complement(12700..13029)
                     /locus_tag="ISB30_003510"
     CDS             complement(12700..13029)
                     /locus_tag="ISB30_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1991110.1"
                     /translation="MKRLQAFKFRLRPGGQQERGMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDPERAYKNFFRLRHHAQT
                     VCYLSRL"
     gene            13052..13576
                     /gene="tnpA"
                     /locus_tag="ISB30_003511"
     CDS             13052..13576
                     /gene="tnpA"
                     /locus_tag="ISB30_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP1991111.1"
                     /translation="MKKETDIRRGRHCVFLKHVHLVFVTKYRRQIFDHDATEKLRTYF
                     SNVCADFEAERVEMDGGPDHVHLLINYPPKLAISSLVNSLKGVSSRLLRRDRPDIAVR
                     YYDKGVLWSPGYFVSSCILTYQLDLITTISVPNRISNVISIPLIIPSTSCNFPTLELH
                     IVQTPDAKKSHVHF"
     gene            complement(13733..>14211)
                     /locus_tag="ISB30_003512"
     CDS             complement(13733..>14211)
                     /locus_tag="ISB30_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1991112.1"
                     /translation="CLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHV
                     NCLLLSDSKQSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSI
                     LDDVRLNNALSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         3524 a         3625 c         3482 g         3580 t
ORIGIN      
        1 ttcaaactat cacataaata agatttatat ataatttata ttattcaggc aatgaattac
       61 ttttgcaagc catcgcattc tcttatgtta ttaatgagtt atgctgattt gttaagcagt
      121 tttatcacgt ttgaaatggc gtcccgcccc gactggtgaa tcgtcggggc tgattttttc
      181 ttattattgc agcagaaact tctccaggaa ctggcgggtg cgaggctgct gaggatcagc
      241 aaataacgct tttgctaccc cctgctcgac tatccgcccc tggtccataa aaatcgcccg
      301 gtccgcaaca tcccgggcaa agctcatttc gtgcgtcaca atcaccatcg tgcgcttttc
      361 ctgcgccagc tgacgaatgg tgttcaggac ttcgcccacc agctctggat ccagcgccga
      421 cgttggctcg tcaaacaaaa tcacttcagg acgcattgcc agagcacgcg caatcgcaac
      481 gcgctgctgt tgaccgccag acaaacgacg tggataactg gtttctttac cggccagccc
      541 aactttcgcc agcagctcgc gagcgcgcgc cgtggcctct tctttcggtt cgcctttgac
      601 gatcaccggc ccttcaataa tgttttccag caccgtacga tgcggaaaca gattaaagtt
      661 ctggaagaca aacccgacgt gctgacgcaa ctggcgaatc agcgattttt gctgacttaa
      721 tgaacgtgca gtatcaatag tgatatcgcc aacggtgatg gttcccgctt ccggttgttc
      781 cagcagattt atgctgcgta gcaacgtggt tttgccggaa ccactcggac cgataattgc
      841 cactacttcg cctggcttca cctcaaggtc gataccatgc agcaccgtct gaccgtggaa
      901 ttttttcacc aggttcttaa cttcaatggc actcatttgg gttctctctc ctggcgatta
      961 agttgattct caaaatggtt ctgcaacgtc gataacacag tcgccatgat ccagtagatg
     1021 agcgaagccg ccaggtacat ggtgaacacc tccagcgtgc gcgaggtaat caactgcgcc
     1081 tgacggaaca gctccggcac ctggattgtc gcggccagcg acgtatcttt taccaggctg
     1141 ataaagctgt tcgacagcgg cggcaacgcc acccgcgccg cctgtggcaa aatcgcgcga
     1201 cgcatggtct gccatggcgt cataccaata ctggctgcag cttcccactg acctttatca
     1261 atagaagaaa tggcggcgcg cagcgtttca gcagcatagg cggcagtgtt cagcgacaga
     1321 ccaatcattg ctgacggaat gggatctaat tcgataccaa actgtggcag tccgtaatag
     1381 atcataaaca gttgtgcgat gagcggcgta ccacgaaaaa tagagatata aaaacgcgcc
     1441 agccagcgca cgggccagat tggcgacagg cgcatcagcg cgagaataaa cccgagcagt
     1501 aagccaaaaa acatgcctcc gatactgagt tgcagcgtat acccagcccc tttcagcagg
     1561 aacggcaagg aatcaataac cagttgtata ctttcttgca tgagcgttcc cggagctaaa
     1621 cgtggggatg ataggcgaac agcgcaggcg caccgccggt atgaataaac aaaatcggcc
     1681 cttcatcttt gaagcgtttc tgactgatac cgtcaatcag ccccgccatc gcttttccgg
     1741 tatacacagg atcaagcaga atgccttcca gcctcgccag caatttcact gcttccatgc
     1801 cttcgtcgtt cggcacgccg tagccaggtg caaaatagtc atcccagagt aaaatttccg
     1861 ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg gaggttaacc actttcggca
     1921 attgatcggc aactgaacgc gacacagtca cgccaatcag ttcgctttca ggcatcagct
     1981 gttccagccc aacagccagt ccggcgtgag ttccggcact gcccgatgcg accaccaccg
     2041 acgaaatatt aaccgccact tcacactgtt gcgcgatttc cagcgcactc tccacataac
     2101 ccagcgcgcc caaagcatta gaaccgccaa ccggaatgac atacgggcga aagccttgtg
     2161 cttcgactcg cgtcgccagc tcttccagtt gggcattggg atcggtcagt gcgtcgcaca
     2221 tttcaatctg ggtattgaac agatccagca acaaacgatt gccgttggtt aaatagtttt
     2281 ccgcggttgt accaatagga ttttccagca gcgccacgca gtgcagaccg agtttcgcag
     2341 cgactgcggc agtctggcgc acatggttag actggatcgc cccggcagta atcagcgtat
     2401 cggcaccttc acgcagagca tctgccgcga gaaattccag cttacgtaat ttattgccgc
     2461 ccattgccat gggagtgacg tcatcccgtt tgatgaaaat ttcccgtcca agataatcag
     2521 aaaagcgcgg cagatattcg agcggcgttg gcgcgccaat aaactccagc cgtggaaaac
     2581 gggttaaatt atgcagtggc ataacagcct ccgatgtgtg ttgttgtgat tttcttatta
     2641 tgcacgctga aaacgcgtaa ataaaaaagg cgctagtgaa agcgcccttt tttgtcatta
     2701 tgctgattat ttggtcacat cagcaccaaa ccatttttcg gaaagggctt gcaaagtgcc
     2761 atctttttgc atttccgcaa ttgcatcatt caccgctttc agcaggtctt catttccttt
     2821 acgcagcgcc acgccagact cctgacgaga gaatgcttca ccggttactg ccagcgtatc
     2881 gttggttttc ttcaccagat ccagcgccgc cagacgatca acgaggatcg catcgatacg
     2941 gcctacgcgc agatcctgat atttggtcgg gtcatcatca taggtacgca catcgacgcc
     3001 ctgaacattc tgacgcagcc actcttcata gttggtgccc agaccgacac ccactttttt
     3061 gcctttcaga tcatcagccg ttttaatggt gccttcgttg ccttttttca ccagcgcctg
     3121 aataccagaa atggtgtacg gggtagagaa atcgtatttt ttcttgcgct cgtcagaaat
     3181 ggtgacctgg ttaatcacca catcaatgcg tttagagtcc agcgacgcca gcataccgtc
     3241 ccatttggtc ggttttagtg acgcctcaac gccaagatgt tttgccagtt gttgggcaaa
     3301 ttccacttca aaaccggtta atttgccgtc atccccctga aaactgaacg gcggataagt
     3361 tccttccagc cctaccagca gcgtgccgcg ctctttgact ttattaagca gaccttcatc
     3421 tgcaaaactt ttaacgctca tacccgcaac cagcgccacg gccatcacac ccatcaatgc
     3481 ctgacgtccc agatgtgcta atttcatatt caccccgaat gttgttatgt ctgtttgcag
     3541 tgtagagcca ttgtttgtaa acacaaaaac aactccgcta catcttattc ttatttaata
     3601 tatatcagaa gcaggcaggc tggaggattt ctgcacaagt ccggtacacg tttgacgctg
     3661 atagtgttga tacttccgta acgcgatgcg gtaattgttg gtgctggtgg agggcagcca
     3721 cggggcgagg atttcatcga ggaaaccgtc ctgcagctga tcgagggaaa tattttgctc
     3781 gtgtagatga ttccccaaac ggcgcagacg aacgacatat tcacgcaccg taccagggct
     3841 catttcagtt tgctcaaaaa gaaattgctt aaagccgata atatcgaaaa aatcactctg
     3901 cgttttgcaa tgtaaatcac cgcaaaatcg gcacaatgcc gcccatgatt gttgttccac
     3961 ttgccagcca ttttcatcaa gcagcgtgtc caggcgggaa atctcgattt tattcactat
     4021 tttgccgtcg cgaacgaggg taatgcgatc gagtaattta cggcaatgcg cgcaatgggt
     4081 ctggctgtgt ttaaagtctt taagatagcg gcttaatggc cgtcttttca ggtgctgcac
     4141 catcattaag aactcctggt agtcaaaatt aaagtgcggc atttactgac gttataactt
     4201 acccagttta gtgcgtaacc gtttaatagc ctggctgtgt aactgactga cccgcgattc
     4261 cccgacctcc agcaccgcac caatctcttt gagattcagc tcttcctgat aatagagggt
     4321 taataccagt ttttcgcgct ccggcaacgt ttcgatggct tccatcaccc gctggcgcag
     4381 attattgtcc agcagttgtt gtagcgggtt ttctcgctga tgatcatcag taaccagttc
     4441 gatgctatcg ccgtgctctt cgcgccactc atcgtaggag aagagctggc tgttattggt
     4501 gtcgagcaac atttggcgat aatcggcaat atcgatccct aaacgttccg ctacctcagt
     4561 ttccgtggcg ttgcggccaa gttcctgctc cagttgccct attgcctgtg ccacttcacg
     4621 cgcgttgcgt cgcacgctgc gcggcaccca gtcacggctg cgaagttcat ccagcatagc
     4681 gccacggata cgctgcactg cgtaagttgt aaatgccgtt ccttgtaggg cgtcatagcg
     4741 ttcgacggca ttaagtaacc caatgccgcc cgcctgtagc agatcgtcaa gttccacgct
     4801 cgcgggcagt cgaacctgca ggcgcaatgc ttcgtgacgc accagcggga cataacgctg
     4861 ccacagcgag tgtttatcca ttacaccttc agcggtatag agtgaattca cgataaacag
     4921 ccctgcgtta tatgagttat cggcatgatt atccgtttct gcagggtttt taatcggacg
     4981 attagtgggt gaaatgaggg gttatttggg ggttaccggt aaattgcggg cagaaaaaac
     5041 cccgccgttg gcggggtcag gcaggttaag gccattagcc ttgtaacaga gacagaactt
     5101 gctgcggtac ctggttggct ttagacagca cggagttgcc cgcctgctgg ataatctgcg
     5161 ctttcgacat gttggacact tcggtcgcat agtcggcgtc ctgaatacgg gactgcgctt
     5221 cagacaggtt ggtggtggtg ttgttcaggt tggtgaccgc agaatccaga cggttttgaa
     5281 cggcaccgag ggaggagcgg aatttgtcga ctttagcgat tgcggcatcc agtgctttta
     5341 acggatcggc tgtagcggct cccgccactt ccgctgcacc aacagttacc gccgccgccg
     5401 taccggtaat gactacagtg gcttccatgt atttaccggt tgcagaatca acgaccgcat
     5461 atttagtact gttagacgcg gtgtcagtgt aagacagtaa attgacttgt ttacccccag
     5521 catccttgac atccgcagca gcaattgctg taccatcagc agccgtcagt ttcgccgcgc
     5581 tgaaatccgc catgccggac acggtaaatc ctttcagatt caaagtggta ctgtcgattt
     5641 gttgcaagcc aatgctgatc gtctggttat cattggcacc aatctgaatc ttcatcgaat
     5701 cgtttttcga cagcacgttc acaccgttga actgggtctg accagataca cggtcaattt
     5761 catcaagacg ggatttgatt tcgtcctgaa tagaagacag gtcggaatca gagttcgtgc
     5821 cggtagaggc ctgaacggtc agttcacgaa tacgctgcaa gttgttgtta atctctgaca
     5881 gcgcgccttc agccgtctgc gccagagaaa taccgtcgtt ggcgttacgt gcggcctgag
     5941 tcagaccttt gatgttagaa gtaaagcggt tagcaatcgc ctggcccgcg gcgtcatctt
     6001 tagcgctgtt aatacgcaga ccagaagaga ggcgctcgat agaagtcgac agcgcagact
     6061 ggtttttgtt gatgttgttc tgagtgatca gcgagaggct gttggtatta atgacttgtg
     6121 ccataattca ttttcctgtt ttcaagtcta cggtttaagt attgggcttc cacccttcgg
     6181 cttcgtcgcc gtcaaccctg ttatcgtctg gtcactcaca acctttagaa tttttttcta
     6241 gcggtcgaaa tactcccctt cctcgttgct gttcgtgcca ttaaggcaaa aaataaatcg
     6301 taaactttac cccgcttacg ccgataaccc cggtattcgt tctatacacg tcgcatgatt
     6361 aaggaaacta tatggctagt atttcaacgc ttggggttgg gtcggggctg gacttaagca
     6421 gcatccttga cagtctggag gcggcagaaa aatctaccct gacgccgata tcaaaacagc
     6481 aaagctctta caccgcgaaa ctgagcgcct acgggacgct gaaaagtgca ctagagagtt
     6541 tccagacagc caacacagca ctgaataaag ccgatttatt taccgccacc tcaaccacca
     6601 gcagttcatc ggctttcagc gccaccacca cgggcagcgc gattgctggg aaatacacca
     6661 ttagcgttag ccaactggcg caggcacaga cgctgacaac aaaaaatagt caaaaagaca
     6721 gtaaagccgc catcgccacc agcgatagcg tactgaccat ccaacaaggc ggcggtaaag
     6781 atccggtcac gattgatatc agcgcgggta actcgtcact ttccggcatt cgcgatgcca
     6841 ttaacaacgc caaagctggt gtcagcgcca gtatcatcaa tgtgggaaac ggcgaatatc
     6901 gtctgtccat caccgctaac gatacgggca gcaataacgc tatgaaactt agcgtcagcg
     6961 gcgacagcgc cctggaaagt tttatgggtt acaacggaac gccgggtgat agcagcaatg
     7021 gcatgattga aagcgtcacc gcgcaaaacg ccaaactgac agtcaataac gttgagattg
     7081 aaaacagcag taacactatc agcgactcac tggaagatat caccctcaac ctgaatgacg
     7141 tcaccaccgg taatcagaca ctgaccatca gtaaagacac atccaaagct gaaaatgcgg
     7201 ttaaagcgtg ggtggatgcc tataacacat tgcaggatac attcagcagc ttaaccaaat
     7261 acaccgctgt cgatgccgga gctgaaagtc aggactccag taatggcgca ttactcggtg
     7321 actccacact gcgtaccatc cagactcagc ttaaaacctt gctcgccaat actcacagca
     7381 gttcaaacta taaaacgctg gcacagattg gcattaccag cgatgccagt accggcaagc
     7441 tggaaatcgc gaccgacaaa ctgcaaacgg cgctaaaaaa tgatgccgct ggtatcggtg
     7501 aaatgtttat aggtgacggc aaaagtactg gggtaaccac cggcattagt agcaacctga
     7561 ccagttggct ctcttccacc gggataatcc aggcagcaaa agatggcgtc agcaaaacgc
     7621 tgaataattt gaccgaccag tacaacgcag ccagtgaacg tatcgatacc ttaatgacgc
     7681 gctataaagc ccagttcacc caattagatg tgttaatgaa ctcgctgaac tccactagta
     7741 gctatctgac acagcagttc gacacctcaa atagcaactc gaaataatct tcaggagaga
     7801 ctatgtacgc ggcaaaaggc acccaggcct atgcacaaat tggcgtcgaa agcgccgtaa
     7861 tgagcgccag ccaacagcag ttggtcacca tgctatttga tggagtgttg agcgcactgg
     7921 ttagagcgcg cctgtttatg caggacaaca atcagcaagg caaaggcgtc tctttgtcaa
     7981 aagcgatcaa catcattgag aacggactgc gggtgagtct tgatgaagag agcaaagacg
     8041 aactaaccca aaacttgatt gctctttata gctatatggt caggcgtttg ctgcaagcca
     8101 atttacgcaa cgatgtctcc gcagtcgaag aagtggaagc attaatgcgc aatattgccg
     8161 atgcctggaa agagtcttta ctctcccctt ctttgattca ggacccagtc tgatgaacaa
     8221 tgcaccgcat ttgtatttcg cctggcaaca actcgtcgaa aaaagccagc tcatgttacg
     8281 cctggcaacg gaagaacaat gggacgaact catcgccagc gaaatggcgt atgtgaatgc
     8341 ggtgcaggag atcgcacacc tgactgaaga ggttgccccg tccactacga tgcaggagca
     8401 gctccgcccg atgctgcgcc tgattctcga caacgaaagc aagataaagc agttattaca
     8461 gattcggatg gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa aatcggtgtt
     8521 aagtgcctat ggcgatcagg gcggctttgt gctggctccg caggataacc tcttttgaat
     8581 cttaatgagc cgatggctcg cgaataatcc gattacggct acgcttctaa tattcccctt
     8641 gaatggagtc gaagaatgcg taatcccacg ctgttacaat gttttcactg gtattacccg
     8701 gaaggcggta agctctggcc tgaactggcc gagcgcgcag acggttttaa tgatattggt
     8761 atcaatatgg tctggttgcc gcccgcctat aaaggcgcat cgggcggcta ttcggtcggc
     8821 tacgactcct atgatttatt tgatctaggt gagtttgatc agaaaggcag catccctact
     8881 aaatatggtg ataaagcaca actgctggcc gccattgacg ccctgaaacg taatgacatt
     8941 gcggtgctgc tggacgtggt agtcaaccac aaaatgggcg cggatgaaaa agaagctatt
     9001 cgcgtgcagc gtgtaaatgc tgatgaccgt acgcaaattg acgaagaaat cattgagtgt
     9061 gaaggctgga cgcgttacac cttccccgct cgtgccgggc aatactcgca gtttatctgg
     9121 gatttcaaat gttttagcgg tatcgaccat atcgaaaacc ctgatgaaga tggcattttt
     9181 aaaattgtta acgactatac cggagaaggc tggaacgatc aggttgatga tgaactcggt
     9241 aatttcgatt atctgatggg ggagaatatc gattttcgca atcatgccgt gacggaagag
     9301 attaaatact gggcgcgctg ggtgatggaa caaacgcaat gcgacggctt tcgtcttgat
     9361 gcggtcaaac atattccggc atggttttat aaagagtgga tcgaacacgt acaggaagtt
     9421 gcgccaaagc cgttgtttat tgtggctgaa tactggtcgc atgaagtcga taaactgcaa
     9481 acgtatattg atcaggtgga aggcaaaacc atgctgtttg atgcgccgct gcagatgaaa
     9541 ttccatgaag catcgcgcat gggtcgcgac tacgacatga cgcagatctt cactgggacg
     9601 ctggtggaag ccgatccttt tcacgccgta accctcgttg ccaaccacga cacccaaccg
     9661 ttgcaagccc tcgaagcccc cgtcgaaccg tggtttaaac cgctggcgta tgccttaatt
     9721 ttgttgcggg aaaatggcgt tccttcggtg ttctatccgg acctctacgg tgcacattac
     9781 gaagatgtcg gtggtgacgg gcaaacctat ccgatagata tgccaataat cgaacagctt
     9841 gatgagttaa ttctcgcccg tcagcgtttc gcccacggtg tacagacgtt atttttcgac
     9901 catccgaact gcattgcctt tagccgcagt ggcaccgacg aatatcccgg ctgcgtggtg
     9961 gtcatgtcga acggagatga tggcgaaaaa accattcatc tgggagagaa ttacggcaat
    10021 aaaacctggc gcgattttct ggggaaccgg caagagagcg tagtgacaga cgaaaacggc
    10081 gaagcgacct tcttttgcaa cggcggcagc gtcagcgtgt gggttatcga agaggtgatt
    10141 taaatccatc cccggcggca agccggggag atttcattac ggcagtggcg tcggtaacgg
    10201 cgctttatcc agcgcggcgg cacactctgc cgtcgggcga tccacacgct ccatcgtcat
    10261 accatcgtac tcaatggtgt taccgtcacg ctcaacctcg tacagctcac gttttacggt
    10321 aacgttagtg agatcatcag acatcaatgt caacttaccc ggcacggcaa tcacccgctg
    10381 ccactgacgg caatccaggg tatcgccctc tttagtgaca atgaggctgc ctatcgcttc
    10441 cggactgacc aacgcacgtt gcggcccttt ggtctgccag taacccgtca gccagtccgg
    10501 cgcgggggtt ttcacgacat tgttatagtt ttcgacttcg gcacagcccg ccagcaacat
    10561 gagcgcacct gcaattgcga gttttttcat cattcgtcct gcatgagaag aaaagaggat
    10621 tgtggcatta aagccctgat gtcgccagcc tttccaggtt cagagagaaa tttgatctaa
    10681 ccattatttt tgtgaattat taccccgaaa ataaatttgc atggtgtatg attcgcgact
    10741 cttgtttcaa accttctgag ttcagaggct acattcatgt catggcagca attcaaacac
    10801 gcctggttga ttaaattctg ggcgcccatc cccgcggtca tcgcggcggg tattctctcc
    10861 acttactatt ttggcatcac tggcaccttt tgggcagtca cgggtgaatt tacccgttgg
    10921 ggcggtcagc tcctgcagtt gttcggcgtc catgctgaag agtggggtta ttttaaaatt
    10981 atccatctgg aaggatcgcc attaacccgc atcgacggaa tgatgatcct cggtatgttt
    11041 ggcggctgtt ttgccgcagc gctgtgggcc aacaatgtca aactgcgcat gccgcgcagc
    11101 cgtatccgca ttatgcaggc catcattggc ggtattatcg ccggttttgg cgcacgtctg
    11161 gcaatgggct gtaacctggc ggcgttcttt accggaattc cacagttctc gctgcacgcc
    11221 tggttctttg ccatcgccac tgccattggt tcatggtttg gcgcgcgctt tacccttctg
    11281 cccatcttcc gtattcccgt gaaaatgcag aaagtttctg ctgcctcacc gttgacgcaa
    11341 aaaccggatc aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt cggcatgctg
    11401 ggctgggcgc tgctgacggc gatgaaccag cccaaactgg ggctggcaat gctgtttggc
    11461 gtcggctttg gtttactgat tgaacgtgcg caaatctgct ttacttcggc gttccgcgat
    11521 atgtggatca ccggacgtac ccatatggcg aaagcaatca ttatcggtat ggcggtaagt
    11581 gccatcggga tcttcagtta cgtacagtta ggcgttgaac ccaaaatcat gtgggcggga
    11641 ccaaacgcgg taattggtgg tttactgttt ggttttggca tcgtgctggc aggcggctgc
    11701 gaaaccggct ggatgtaccg cgcggtagaa ggccaggtgc actactggtg ggtcggtctg
    11761 ggcaacgtga tcggctcaac gattctggcg tactactggg atgatttcgc tccggcgctg
    11821 gccaccgact gggacaaaat caacctgctg aaaacctttg gcccgatggg cggcctgctg
    11881 gtgacatatt tgctgttgtt tgctgctcta atgttgatta ttggctggga aaaacgcttc
    11941 ttccgccgtg cggcaccgca gactgctaag gagatcgcat gaaaaatatc gttcctgatt
    12001 accgtcttga tatggtgggt gaaccctgcc cttatccggc agtcgcaacc cttgaggcga
    12061 tgccgcagtt aaaaaaaggg gagatcctgg aagtggtgag cgactgtccg cagtcgatca
    12121 ataatattcc actggatgcg cgtaatcacg gctatacggt gctggatatt cagcaagacg
    12181 ggccgaccat tcgttattta attcagaagt aatccctcat tcccgctgtg gcccacagcg
    12241 ggaacatcgt tacctctcta tcacatagat tttacccgcc ctctctccta ttcttttcag
    12301 tgtgctctca ggaggtttct atgtgtggac gctttgccca atcccaaacg cgtgaagatt
    12361 acctcgcgtt tctcgcggaa gatattgaac gcgatattcc ctacgatccc gaacctattg
    12421 gcagatacaa cgtcgcgccg ggaaccaaag tccttctgct cagtgaacgt gatgaacacc
    12481 ttcatctgga tccggttttc tggggatatg ctcccggatg gtgggataaa ccgccgctga
    12541 ttaacgcccg cgtagaaact gccgcaacca gtcacatgtt taaaccgctc tggcaacatg
    12601 gtcgggcaat ctgttttgcc gatggctggt ttgagtggaa aaaagaaggc gacaaaaagc
    12661 agccttattt tatctatcgt gctgatggac aagctgtttt tatagccgcg ataggtagca
    12721 caccgtttga gcgtggtgac gaagccggaa gaagtttttg taagcccgct cagggtcttt
    12781 cagtgactgt tgcaatggct gtgagggcga atctttaagc cattgcgttt cagtggcatt
    12841 tttccactca accaaccagg aagccatttt cccgtaaggg atgtatttat taccggcctc
    12901 atgattctca ttctgaagcg ccagagcacg attgaaaacg aaacgacatg cgcctgcgaa
    12961 gcgcctcatt ccacgctcct gttgaccacc tggtcttaac cggaatttga atgcctgtag
    13021 tcgtttcatg ggccaaatca tactttggcc tatgaaaaaa gaaaccgata tccgacgcgg
    13081 aaggcattgt gttttcctga agcatgttca cctggtcttc gttactaaat accgacgtca
    13141 gatttttgac catgacgcga cagaaaaact acgcacttac ttttcaaatg tatgtgctga
    13201 ttttgaagct gaacgggttg aaatggatgg cggaccagat cacgtccatt tgttaattaa
    13261 ctatcctccc aaactggcga tatccagtct ggtaaacagc ctcaaaggcg tatcgagtag
    13321 gttactgcga cgagatcgac cagatattgc agtcaggtat tacgacaaag gcgttttgtg
    13381 gagtcctggc tattttgtca gtagctgcat cttaacgtat caactagatt taataacaac
    13441 gatatccgta cctaaccgga tatctaatgt cataagcatc cctttaatta tcccctcaac
    13501 ctcttgtaac tttcccactc ttgagctcca catagtccaa acgccggatg caaaaaaatc
    13561 acacgtgcat ttctgaaatt gtgatggagg aagatagtat caattatatg tgcttagaaa
    13621 cgtccccgat tagcgcagaa gtcaccggat tgttcagact ccaataggtt gagtatgccg
    13681 tatttatttt ccaaaataaa aatcgatgct gttgctcctt cataaaatac ttttactctc
    13741 tggacgcttt tcttagctct cttggtgtta tcttaaaacg attatggaac ctttcagtaa
    13801 aacgagaagg acacttgtaa ccattttccc tggcaatctc gctgatgggt tttactgtcg
    13861 tttgtatagc agacaacgca ttatttaacc tcacgtcgtc caatatactt tgaaaactta
    13921 ctccctcgct tgccagacgg cgatgcaatg tggaaacaga aatgtagaga tatctcgcta
    13981 ccttgtttgc tgtccatttt gttccaggtt cggatagcat taggttataa caacgactta
    14041 ttaatgattg tttactatct gataaaagta aacaattaac atgatgtgct cctaatgaaa
    14101 gtagaacgcc cattgctaag tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg
    14161 tttttagttg ctcccaacaa tatattaact cggggttctg aggtaagcag a
//
LOCUS       DACADF010000120        14160 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.122, whole genome shotgun sequence.
ACCESSION   DACADF010000120 DACADF010000000
VERSION     DACADF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.122"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..645
                     /gene="gspC"
                     /locus_tag="ISB30_003513"
     CDS             <1..645
                     /gene="gspC"
                     /locus_tag="ISB30_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HAP1991113.1"
                     /translation="SEFSLAALWRNENHTGVKDANPVAVNQETPKLSIALNGIVLTSN
                     DETSFVLINEGNEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPD
                     IIKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINN
                     LSLTHPDEISQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRNEGTQ"
     gene            629..2581
                     /gene="gspD"
                     /locus_tag="ISB30_003514"
     CDS             629..2581
                     /gene="gspD"
                     /locus_tag="ISB30_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HAP1991114.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            2591..4072
                     /gene="gspE"
                     /locus_tag="ISB30_003515"
     CDS             2591..4072
                     /gene="gspE"
                     /locus_tag="ISB30_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HAP1991115.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNIPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQTVIRGDTSWDEVMRVASATLENKA"
     gene            4069..5265
                     /gene="gspF"
                     /locus_tag="ISB30_003516"
     CDS             4069..5265
                     /gene="gspF"
                     /locus_tag="ISB30_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HAP1991116.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5275..5712
                     /gene="gspG"
                     /locus_tag="ISB30_003517"
     CDS             5275..5712
                     /gene="gspG"
                     /locus_tag="ISB30_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HAP1991117.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5720..6229
                     /gene="gspH"
                     /locus_tag="ISB30_003518"
     CDS             5720..6229
                     /gene="gspH"
                     /locus_tag="ISB30_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HAP1991118.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKPDDSNQPQVVILADGQITPFSLLMANTGTGEPLLTLVSSGSWPLD
                     QTLARDTRP"
     gene            6226..6603
                     /gene="gspI"
                     /locus_tag="ISB30_003519"
     CDS             6226..6603
                     /gene="gspI"
                     /locus_tag="ISB30_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HAP1991119.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDSFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            6596..7183
                     /gene="gspJ"
                     /locus_tag="ISB30_003520"
     CDS             6596..7183
                     /gene="gspJ"
                     /locus_tag="ISB30_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HAP1991120.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7176..8159
                     /gene="gspK"
                     /locus_tag="ISB30_003521"
     CDS             7176..8159
                     /gene="gspK"
                     /locus_tag="ISB30_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HAP1991121.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDADDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTSELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8174..9337
                     /gene="gspL"
                     /locus_tag="ISB30_003522"
     CDS             8174..9337
                     /gene="gspL"
                     /locus_tag="ISB30_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HAP1991122.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLGSLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVGKSGKWLTRLAVSCLVLAILSFVGSRGIALWQTLKIEDQL
                     QQQQQETWQRYFPQIKHTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHSELQLMEA
                     NYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9334..9795
                     /locus_tag="ISB30_003523"
     CDS             9334..9795
                     /locus_tag="ISB30_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HAP1991123.1"
                     /translation="MIKSWWAEKSTSEKQIIAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEITLKHLSFREQQ"
     gene            9795..10467
                     /locus_tag="ISB30_003524"
                     /pseudo
     CDS             9795..10467
                     /locus_tag="ISB30_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(10496..10972)
                     /gene="bfr"
                     /locus_tag="ISB30_003525"
     CDS             complement(10496..10972)
                     /gene="bfr"
                     /locus_tag="ISB30_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAP1991124.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11044..11238)
                     /gene="bfd"
                     /locus_tag="ISB30_003526"
     CDS             complement(11044..11238)
                     /gene="bfd"
                     /locus_tag="ISB30_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAP1991125.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            complement(11407..14109)
                     /gene="chiA"
                     /locus_tag="ISB30_003527"
     CDS             complement(11407..14109)
                     /gene="chiA"
                     /locus_tag="ISB30_003527"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HAP1991126.1"
                     /translation="MKLNIFTKSMIGMGLVCSSLPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSTSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGSIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEITQYGNPGSCSVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADS
                     SNDYSLQAWSGQEGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTAT
                     AAEMSQYGNPTTCEIDNGGVIIVDGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVP
                     GGDNPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNS
                     RPWKPLGKTQSYSNEELNNAPQFNPETLYASDTLIRFNGENYISQSKVQKVSPSDSNP
                     WRVFVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVV
                     SKDANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDL
                     MQHYYDIVDNLNLRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTL
                     PILPTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSA
                     IANLHAQLKGLYGNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLG
                     MVGIWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT         3650 a         3644 c         3354 g         3512 t
ORIGIN      
        1 tctgaattca gccttgccgc tttatggcgc aacgaaaatc atactggggt aaaagatgca
       61 aatcccgtgg cagttaatca ggaaacacct aagctctcta tagcgcttaa tggcattgta
      121 ctcacatcga atgatgagac ttcttttgtc ttaattaatg aggggaacga acaaaagcga
      181 tactccctca acgaagccct tgagtcagcg ccgggaacgt ttattagaaa aataaataaa
      241 accagcgttg tgtttgaaac tcacgggcat tacgaaaaag taactctcca tccagggcta
      301 cctgacatca tcaagcagcc tgattcggaa aatcaaaatg tactcgccga ttatattatc
      361 gccacaccta tacgtgatgg agaacagata tacggtcttc gattgaatcc tcgtaagggt
      421 ctcaatgcat ttaccaccag tttgctgcaa cctggggata tcgccctcag gatcaataat
      481 ctttcgctga cccaccctga tgagatttca caggcattaa gtttactgct aactcaacaa
      541 agtgctcagt ttacaattcg tcgcaacggc gtaccccgct tgataaatgt ttccgtcgcg
      601 gaacttacag gaatgaatgg acagcgcaat gaagggactc aataaaatca cctgctgctt
      661 gctggcagca ttactcatgc cttgtgcagg acacgctgag aacgaacaat acggcgctaa
      721 cttcaataac gccgatatcc gccagttcgt ggaaatagtg ggtcagcatc ttggcaaaac
      781 gatcctgatc gacccttcgg tacagggaac catttccgta cgcagtaatg atacgtttag
      841 ccaacaggag tactaccagt tctttttaag tattcttgat ctttacggtt attccgtgat
      901 cacgctggac aatggttttc tgaaagtggt gcgctcagct aatgtaaaaa catcgccagg
      961 gatgattgct gacagttctc gtccaggcgt aggtgatgag ttggtcaccc gaatcgtacc
     1021 gcttgagaac gttcctgctc gtgacctggc tcccctgctc cgccagatga tggatgcggg
     1081 tagcgtcggt aatgttgtgc attatgaacc ctccaacgtt cttattctga ccggtcgtgc
     1141 ctccaccatt aataaactga ttgaagtcat aaagcgcgtt gatgtcatcg gcacagagaa
     1201 gcagcaaatt attcatctgg aatatgcatc agcggaagat ctcgccgaga ttcttaatca
     1261 attaatcagc gaaagccacg gtaaaagcca gatgcctgcc ctcctttccg cgaagattgt
     1321 ggcggataag cgaaccaact ctcttatcat cagcggaccg gaaaaagcac gccagcgcat
     1381 aacttcatta ctgaaaagcc ttgatgtaga agagagcgag gaaggaaata cccgggtcta
     1441 ttacctgaaa tatgctaaag ccacgaatct ggtggaagtg ctaaccggtg tttccgaaaa
     1501 gctgaaagat gaaaaaggga attcgcgtaa gccttcttct acttctgcga tggataatgt
     1561 ggccattacc gccgatgaac agaccaactc gttggtcatt accgctgacc agtccgtcca
     1621 ggagaaactc gccacggtaa ttgcgcgtct ggatattcgc cgtgcacagg tgctggttga
     1681 ggcaatcatc gtcgaagttc aggatggaaa tggactcaac ctcggcgtgc aatgggcgaa
     1741 taaaaacgtt ggcgcacagc aatttaccaa tacgggatta ccggttttta acgctgcgca
     1801 aggtgtagct gattataaaa agaatggcgg gatcaccagc gcgaatcctg cctgggatat
     1861 gtttagcgcc tacaatggca tggctgcagg cttcttcaat ggcgactggg gcgtattgtt
     1921 gactgcgctg gccagtaaca ataaaaatga catcctcgcc accccgagca tcgtaacgct
     1981 ggataataaa ctcgcgtcct tcaacgttgg tcaggatgtg ccggtgctat ccggctctca
     2041 gaccacttct ggggataacg tctttaatac tgtcgaacgc aaaacggtgg gcacaaaact
     2101 caaagtcact ccgcaggtca atgaaggcga cgcggtactg ctcgaaatag aacaggaggt
     2161 ttccagcgtt gactcttcgt ctaattcgac gctcggcccg acatttaaca cccgtactat
     2221 tcaaaacgcc gtgctggtta aaaccggtga aacggtggtc ctgggcggat tgctggatga
     2281 tttttctaaa gagcaagtgt caaaggttcc tctgcttggc gatattcctt tagtgggaca
     2341 actcttccgc tatacctcga ccgagcgcgc taaacgcaac ctgatggtat tcatccgccc
     2401 gacgattatc cgtgacgatg atgtttatcg ctcactgtca aaagagaaat acacccgcta
     2461 ccgtcaggag caacagctgc gaatcgacgg gaaatcaaaa gcgctggttg gctcggaaga
     2521 tttgccggtg ctgaatgaga acacgttcaa cagtcacgcc cctgcgccat cgtcacggtg
     2581 aggcattctt atgagaattc actcaccgta ccccgccagt tgggcgctgg cacaacgaat
     2641 tggttatctc tattcagagg gcgagattat ttatctcgcc gatacgccat tcgaacggtt
     2701 actcgatatt caacgtcaag ttggtcagtg ccagaccatg accagcttgt cacaggctga
     2761 ttttgaagcc cgactggaag cggtgttcca tcagaatacc ggtgagtcgc aacagattgc
     2821 gcaggatatc gatcaatccg tcgatcttct ctcgctttcg gaagagatgc ccgcaaatga
     2881 agatctcctg aatgaagatt cagcggcacc ggttatccgc ttgatcaatg ctattttgag
     2941 tgaggccatc aaagaaaccg cctccgatat ccacattgaa acctatgaaa aaacaatgtc
     3001 gatccgtttt cgcatcgacg gcgttttgcg gacaatttta cagcccaaca aaaaactggc
     3061 ggcactgctt atctcccgaa ttaaggtcat ggctcgtctt gatatcgccg aaaaacgtat
     3121 tccacaggat ggaaggatca gtttgcgtat cgggcgacgt aacatagatg tccgcgtatc
     3181 cacactgcca tccatctatg gtgaacgcgc tgtgctccgt ctgctggata aaaacagcct
     3241 ccagctttca ttgaacaacc tggggatgac ggcagcggat aaacaggatt tagaaaatct
     3301 cattcagctt ccgcacggta ttatcctggt gacagggccg acaggctccg gtaaaagcac
     3361 cacgctctac gccatccttt cggcgcttaa tatccctggc cgtaatattc tgacggtaga
     3421 agatcccgtg gaatatgagc tggaaggcat tggacaaacg caggtgaata cccgtgtgga
     3481 tatgtctttc gctcgcggcc tgcgcgccat acttcgccag gacccggatg tcgtcatggt
     3541 gggggaaatt cgtgatacag aaaccgcgca aattgcggtt caggcctcgc tcacaggcca
     3601 tctggtactc tcaacactcc acactaacag tgcatcaggc gcagtcaccc ggctacgcga
     3661 catgggcgtc gaatcattcc tgctttcatc ttcactggca gggattatcg cgcaacgtct
     3721 ggttcgtcgt ctgtgtccgc aatgccgaca attcacgccc gtatcgcccc agcaagcgca
     3781 gatgtttaaa tatcatcagc tcgcggtgac aacaattggc actcccgtag gttgccctca
     3841 ttgccatcaa tccggttatc aggggcgcat ggcgatccac gaaatgatgg tggtgacgcc
     3901 ggaattgcgg gccgctattc atgaaaatgt ggatgaacaa gcactggagc gactggtccg
     3961 gcaacaacac aatgccttaa tcaaaaatgg cctgcaaaca gtgatacgcg gtgacacctc
     4021 ctgggatgag gttatgcgcg tcgccagtgc tacgctggag aacaaagcat gaattatcgc
     4081 tatcgcgcca tgacccagga tggtcaaaaa ttgcagggga tcattgatgc taacgatgaa
     4141 cgtcaggcac gactgcggct gcgtgaagaa gggcttttcc tgttggatat tcgcccccaa
     4201 aaaagttcgg gagtaaaaac acgtcgcccg aggatcagcc atagtgaact gacgcttttc
     4261 acccggcagt tggcaacctt aagcgcagcg gcattacccc tggaagagag ccttgccgta
     4321 attggtcaac aaagcagtaa taaacgactg gctgacgtgt taaatcaggt acgcagcgcc
     4381 atccttgaag ggcatcccct ttcagatgca ttacagcatt tccccacgct tttcgattcg
     4441 ctctaccgta ccctggtaaa agcgggcgaa aagagcggtc tgctggcccc ggtgttggaa
     4501 aagctggctg attacaatga aaaccggcag aaaatccgca gcaagctcat tcagtcactg
     4561 atctacccct gcatgctcac tacggtggcg attgtggtcg tgattattct cctcactgct
     4621 gtcgtgccca aaattaccga acagttcgtg catatgaagc agcaactgcc actgagtaca
     4681 cgcattcttt taggtctgag cgacacgttg caacgtaccg gcccgacatt attagcgaca
     4741 gtatttattg tcgctgtggg tttctggctc tggttaaaac gcggcaataa ccgccaccgt
     4801 tttcatgcca tgttactgcg cgttgcgctc atcggcccgc tgatttgcgc cattaacagc
     4861 gcacgctatc tccgcacttt aagtattttg caatccagcg gcgtccctct gctggatggg
     4921 atgaatttgt ccaccgaaag cctcaacaac ctcgaaattc gccagcgcct ggcaaatgcg
     4981 gcagaaaacg ttcgccaggg taacagcatt catctttcac tggaacaaac cgcaattttc
     5041 ccgccgatga tgctctacat ggtggcctct ggcgaaaaaa gcgggcaact cggcacgtta
     5101 atggtcagag ccgcagataa ccaggagaca ctccaacaaa atcggatcgc cttaacgctc
     5161 tccatcttcg agccagcact cattattacg atggcactga tcgtcctgtt tattgtcgtg
     5221 tcggtactcc aacctcttct tcaacttaac tcaatgatta attaaggaaa tcatatgcgc
     5281 gcaacggata agcaacgcgg ttttacatta ctggaaatta tggtggtaat tgtcatcatt
     5341 ggcgtactcg ccagcctggt ggttcccaac ctgatgggca ataaagaaaa ggcggataaa
     5401 caaaaagccg tcagcgatat tgtggcgctg gaaaacgccc tggatatgta caagctcgac
     5461 aatcatcgct atccaaccac aaatcagggg cttgaatcct tagtagaagc gccgacactg
     5521 ccaccgctgg ccgcaaacta taacaaggaa ggttatatca agcgtctgcc tgccgatccc
     5581 tggggcaatg attatgtcct cgttaatcct ggtgaacatg gtgcatacga tctgctttca
     5641 gcaggacccg atggtgaaat gggaaccgag gacgacatca ccaactgggg tttgagcaag
     5701 aagaaaaagt aagtcagcga tgaatcagca acgcgggttt accctgctgg agatgatgct
     5761 ggtgctggcg ctggtcgcta tcacggcaag tgtggtgctc ttcacatatg gtcgggagga
     5821 tgccgccagc actcgtgcgc gcgaaacagc ggcgcggttt accgctgcac ttgaattagc
     5881 catcgaccgc gccacgctca gtggtcagcc tgtcggtatc catttctctg attcagcatg
     5941 gcgcattatg gtaccgggca aaacaccatc agcctggcgc tgggttccat tacaagaaga
     6001 tgccgcagac gagagcaaaa atgactgggg tgaagaactc tcaattcaac ttcagccatt
     6061 taaacctgat gacagcaacc aaccgcaggt ggtgattctg gccgacggac aaatcacgcc
     6121 cttctctttg ttgatggcta ataccgggac cggtgagccg ctcctgacgt tagtcagttc
     6181 aggctcatgg cccctggatc aaacgctcgc cagagatacc cgcccatgaa caaacaatca
     6241 gggatgacac tgcttgaagt cttactggcg atgagtattt tcaccgctgt tgcgctgact
     6301 ttaatgagca gtatgcaagg gcaacgaact gccatcgagc ggatgcgtaa cgagacgctg
     6361 gcattgtgga tagcggataa ccagcttcag tcacaagatt cattcgacga ggaaaacacg
     6421 tccagttctg gcaaagagtt aataaatggc gaagagtgga actggcgcag cgatatccac
     6481 tcaagcaaag acggcacgct tctggaacgc accataacgg tgacattacc cagcggccag
     6541 acaacctcgc ttacgcgcta tcaaagcatc aataataaat ccgggcaggc acaagatgat
     6601 taatcgccag cagggtttca cattgctgga agtcatggcg gcgctggcga ttttctcaat
     6661 gcttagcgtg ctggcgttta tgattttttc tcaagcttcc gagttgcatc aacgttcaca
     6721 gaaagaaatc cagcagttca atcagctaca gcgcaccatc accattcttg ataacgatct
     6781 cctgcaactg gtcgcccggc ggaatcggag tacggacaaa atcatggtgt tgggtgaaga
     6841 agcgattttt accacgcaaa gtcgcgatcc gctggcccct ctcagtgaag cacaaacctt
     6901 actcactgtt cactggtatc tgcgaaatca tacgctctac cgtgctgttc gtacttctgt
     6961 ggatggcagg aaggatcaac ccgcccaggc gatgctggaa catgtcgaga gctttcttct
     7021 ggaaagtaac agcggagaaa gccaggagct tcctctctca gtcacgttac atctgaagac
     7081 tcagcaatat ggggcgttac agcgccgttt tgcattacct gagcagttag cgagagaaga
     7141 gtcgccagct cagacgcagg ccgggaataa taaccatgaa taatgaacaa cgcggcgtag
     7201 cactgctcat tgtactcatg ttactggcgc tcatggctgc tctggctgca gacatgacga
     7261 tcagttttca cggccagctt catcgaaccc gccaggtaaa ccatcatttg caacggcaat
     7321 atgatattga gctggcggag aaacttgcgc ttgccagcct cacccaggac gttaaagata
     7381 acgacagaca aaccacgttg cagcagtact gggcacaacc gcaacagttg cagctggaaa
     7441 atggcaatac agtgaagtgg cagttacgcg atgcccagca ctgttttaat ctcaatgcct
     7501 tagcaaaaat ctctgacgcc ccgctggctt cccctgattt ccccgcccag gtattcagtg
     7561 ccctgctgat taacgccggt atcgatcgcg gaaacaccga tgagatcgtg caatcaatcg
     7621 ctgactatat tgatgccgat gattcgccac gctttcacgg cgcagaagat aacttttacc
     7681 aaagccaaac gccgcctcgc catagcgcca atcaaatgct ttttctgacc ggtgaattgc
     7741 gtcagataaa aggcataacc gaaaacatct atcagcggct tatcccgtat gtctgcgttc
     7801 tcccgacctc ggaacttagc atcaatctta atatgctaac ggagaacgat attccgctct
     7861 tcagagcatt attcctcaat aacatcacgg atgctgacgc tcgggtctta ttacaaaaaa
     7921 ggcccaggga aggctggcta accacagatg catttcttta ctgggcgcag caggatttct
     7981 ccggcgtgaa gcccctggtt gctcaggtga aagggcatct ctttccctat agccgctatt
     8041 ttacgctgag tacggaaagt atcagcgacg aacaatccca gggatggcaa agccatattt
     8101 ttttcaaccg taaacagcag agcgcccaaa tctatcggcg tacgctgcaa ctctactaat
     8161 cataaggatc gtgatgcctg aatcgttgat ggtaatacgt tctttctcga cgctgcgtaa
     8221 gcattgggaa tggatgacgt tctccgcaga tagcgtatct tcggtacata cgctcacaga
     8281 cgatcttccg ctggggtcgc tggccgatca gcccggtgcc ggaaatgttc accttttgat
     8341 cccacctgag ggtcttttat atcgttccct cacgctgccc aacgccaaat acaaactcac
     8401 cgcgcaaaca ttacagtggt tggcggagga gacattaccc gacgccagcc aaaattggca
     8461 ctggacggtt gtcgataaac aaaacgaaag tgtcgaggtg ataggcattc agtcggagaa
     8521 actcagtcgt tatcttgagc gtttacatac tgccggactc aacgtgacgc gggttttgcc
     8581 agatggctgc tatcttccct gggaagtaga tagctggact ctggttaacc agcaaacaag
     8641 ctggcttatt cgcagtgccg cacatgcctt caacgaactg gatgaacact ggttacagca
     8701 tcttgctaac cagtttccgc ctgagaatat gcgttgttat ggtgtagcgc ctcacggcgt
     8761 tgcagcggcc aatccactta tccagcatcc tgaaatacca tcactgagtt tgtattctgc
     8821 cgatattgca ttccagcgct atgacatgct gcatggtgtt tttcgtaaac agaaaactgt
     8881 aggcaaatcg ggaaaatggc tcacccgcct ggcagtaagc tgtctcgttc tcgccattct
     8941 ttcttttgtt ggcagccggg gcatcgcgct ctggcaaacg cttaagattg aagatcaact
     9001 tcagcaacaa cagcaagaaa cctggcaacg ttatttcccg cagatcaaac acacccacaa
     9061 ctttcgtttt tactttaagc agcaactcgc tcagcaatat cctgaagctg taccgttgct
     9121 ctatcattta caaacacttc tgcttgaaca ctctgaactg cagttaatgg aggcgaacta
     9181 tagccagagg caaaagtcgc tgaccttaaa aatgagtgct aagagtgaag ccaatatcga
     9241 tcgtttctgt gagttaacac agtcctggct tccgatggaa aaaacagaaa aagatccggt
     9301 cagtggtgta tggacagtaa ggaactcagg gaaatgatta aatcatggtg ggcagaaaaa
     9361 tccacatcgg aaaaacagat cattgcggca ctggcggttc tctcgctcgg cgttttttgt
     9421 tggcttgcgg tgataaaacc gatagatacc tacattgaag agcatcagtc ccacgcgcaa
     9481 aaaattaaga aggacatcaa atggatgcag gatcaagcga gcacccatgg tttactcggt
     9541 catccagcgc ttacgcagcc catcaaaaat atccttctcg aagaagcaaa acgtgagaat
     9601 ttgactatca cgctggaaaa cggtccggac aatactctga caattaatcc tgtcacagct
     9661 cccctcgaaa acgtctcccg ctggctcact acggcacaga taacatacgg tattgttatt
     9721 gaagaccttc agtttactct tgccggaaac gaagagatca ccctcaagca tttgtccttc
     9781 agggagcaac aataatgaca atgttactgc cgctcttcat tctggttggt tttattgcag
     9841 gttattttgt taatgccatg gcctatcacc tctcgccttt ggaggataaa acggcgttaa
     9901 cgtttcgcca ggtattggtt catttctggc aaaaaaaata tgcctggcat gatacagtgc
     9961 ccctgatact ttgcgttgct gctgctatcg cctgcgccct ggcacctttc acgcccatcg
    10021 tgactggcgc actctttctc tatttctgtt tcgcactcac gctcagtgtt attgattttc
    10081 gcactcagct cctgcccgac aaactcacct taccgctgct ctggcttggt atttaatgcg
    10141 cagtctggat taattgattt acatgatgcg gtttacggcg cggtcgctgg ctatggggtg
    10201 ctgtggtgtg tttactgggg cgtctggtta gtttgtcaca aagagggatt gggctacggt
    10261 gatttcaagc tactggccgc tgcaggcgcg tggtgtggct ggcaaacgtt gccaatgata
    10321 ctgctgattg cctcgctggg tggcattggt tacgccatcg tttcacaact tctgcaacgc
    10381 cggactataa ccactatcgc attcggcccg tggcttgcgc tcggcagcat gataaacctg
    10441 ggatatctgg cctggatctc ttattaaccg ggagggttct ccctcccgac acggctcaac
    10501 cttcttcgcg aatctgtgct tgcagataat tttgcagtcc catcttctga atcagatcaa
    10561 gttccgtttc cagccagtcg atatggcctt cttcatcgcg caaaatttct atcatcatat
    10621 cgcggctgac gtaatcatga acgctatcgg cataaccaat tgcctcacgc aaattcttcg
    10681 cgccatccag ctcaagtgcc agatcagaac gcagcatttc ctcaacatct tcaccaatgt
    10741 tcagtttgcc caggtcctgt aagtttggaa gaccttccag aaaaagaatg cgctctatat
    10801 aacgatcggc gtgtttcatc tcatcaatgg attcatgata ctccacatca ttgagacgtt
    10861 tgagacccca gtttttaaac atccgggcat gaagaaagta ctgattgatt gcgacaagct
    10921 catttcccaa cagtttgttg agataattta taactttagt atcacctttc attttttgac
    10981 tccttcgctt ccactctttg aagcgtagaa cggcttacga gtaagtcaaa aaacacactc
    11041 cgtttatgcg gactccttaa actccggcag ttgcattaat tcatcctcca tcacttcacg
    11101 cgcggcccgg acacatttac cgcactgatt gccgaccgga atgaattttt ttaattgctg
    11161 gaacgagtgg ggggaaaact ggcgcacagc ctgacgaatt tttttatcgc tgataccatt
    11221 acaaagacaa acgtacatag agactcccgt atactttctt caaaagtaaa tgaaaatagt
    11281 tcttatttca attgcaggaa gactcttttt taagcaaaga atagaaatat gaataaaaat
    11341 aatgggctac caaaagtagc ccattgacaa aaaatgcggc gataccggaa ggtatcgcca
    11401 acacatttat tgcttagtaa acggcgcgaa gatttcgcta aatgcgtact tcggagcctg
    11461 ttctttagtc agaccgtgga attccggaga caggttagtg ccgcccggca ggtcgcgcgc
    11521 gattgaccag ataccaacca taccgagatt acgcttctgc gcatcctgca tgaccagacg
    11581 agcatcagag agatagaaca cctcaccctg aacgtcgttc acgccaacca tcggcgtggt
    11641 acccatcatg gcgtcaattt ctgcatcact cttattgcca tagaggcctt tcagctgcgc
    11701 atgcaggttg gcaatcgcag acgttgcaca cttaccgtgg atgttttggc cttcggtgtt
    11761 tgcagactga caaatagcgt taccgtagtc cattgtcatc acgttcacac ccgccagctc
    11821 aacacctttc gctttggcat cgctcaggac attcatccct tccggcgtca ggcctgtcgg
    11881 cagaattggc aaggtatacc agatagcaat atctttgcct tctgacttcc atttatcctg
    11941 cactttcttc acagcaaggt tacgacgttc aatagatgcc tgatccgcaa cccaggtgcc
    12001 ttcgatatcg aagtccagga ctctgaggtt caggttatca acgatgtcat aataatgctg
    12061 cattagatcg tcaacgttct tacaggaagc tgccagcggg gcattattag caccaccgat
    12121 agacagcatc acatcaccgc cagcctcacg cagcgctttg attttgctgt actgagcgta
    12181 attctgcata ccgtaagcgg tcccccatgt cggcagacag gtgttcgcat ctttactcac
    12241 cacaaacgcc agcgtgaagt ggttgacgtt atgattctta gccagcgcag ccagatccgg
    12301 gatcgtattc agcgtaaagt cgacatacgg tgcataaacg tgtttcggcc acgctttctt
    12361 cggcgtacct acgcgctctt tggttccggt ccagtcaaca aagacacgcc acgggttgct
    12421 gtcagaagga gaaactttct gcactttact ctgagaaatg tagttctcac cgttaaagcg
    12481 aatcagcgta tcactggcat aaagcgtttc tggattaaac tgcggcgcat tattcagctc
    12541 ttcgttgcta tagctctgag tcttacctaa cggcttccac gggcgactat tgctaccggt
    12601 ggcgttttgg ttcgcaacca gagcagggtt gtcactttgc gtccagaaca gcgcttcata
    12661 agcctgccca tcaacaacaa cgcgatcacc tttcacgtac acggtggaag cggaccatgc
    12721 tttcgccggt tcgtatttct tccacgggtt atcgccgccg gggacaaagc cccatgcgtc
    12781 ttgatcgaca gaggttttat aatgtgcatc gttataatct acgatgctgt tcgcgctata
    12841 cgctttgctg gcctggaaac catccacaat aatgacgccg ccattatcaa tttcacaggt
    12901 agtcgggtta ccgtactgac tcatttccgc agcggtagct gtacgcacga gacgccatgg
    12961 gttattggag ttttctacac tggtgccacg cgggcaatct tcagaaccaa cccaccaggc
    13021 attctgataa acattaccat tgaagataac atggtaaatt tcgctgcctt cctggccact
    13081 ccacgcctgc agtgagtaat cgttactgct atccgctggt gttgatggct cgttgttatc
    13141 cggagttgga gtaactggag tttccggtgt ttccggagtt ggatcaaccg gagtcacagc
    13201 accgccatta ttatccggct taacggaaca ggagcctggg ttaccatact gagtgatttc
    13261 tgttgccgtt gccgcgcgaa cataacgcca tgggttgctg gcatcgtttt ctttcgcatc
    13321 gcctgggcaa tttgccgcgc ctacccacca ggcgttctta taaatgctgc cgttgtagat
    13381 aacgtaccat gtagaagaac cttcctgacc tttccaggct acaacagcat agttgtcgtt
    13441 tgacttatca gctggggctg gctgaacatc aggggccggt ttaacgtccg gtgccggttt
    13501 cacatccggt gcaggctgtg gtgctgaagt acaagactgt ggattactgg tttctgagat
    13561 ttccgttgcc gtagcggcac gaacataacg ccatgggttg ctggcatcat tgcctttcgc
    13621 atcacccggg cagttagaag aggctaccca ccaggcattt ttatataccg caccattaaa
    13681 gacgacatac caggtttgac cgccctgctg tttattccag gcaactacag aagaattaga
    13741 cggaacagtg ccctgtgctg gtgtagccga accgccatta gcgggcgtat tagatgctgg
    13801 cgtatttgaa gttgaagaag atgagcttcc gctcttttcg caggaaagtg tattgccaaa
    13861 ctgactaatt tcagcagctg ttgcggtacg ctttaaacgc cacgggttag ttgcatcatt
    13921 tgctttcgct tttcccgggc aatttgtaga agaaacccac caggcatttt cataaatttt
    13981 gccatcgaaa ataacctgat atttattacc accttgttga ttattccatg cttccattgc
    14041 caatgctggc agagaggaac acaccagccc cataccaatc atagatttag taaatatatt
    14101 taatttcatt ataatccctt gtaacgtaaa aactgcaaaa caaaagtcct acgcaggaat
//
LOCUS       DACADF010000121        14083 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.123, whole genome shotgun sequence.
ACCESSION   DACADF010000121 DACADF010000000
VERSION     DACADF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.123"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..476)
                     /locus_tag="ISB30_003528"
     CDS             complement(<1..476)
                     /locus_tag="ISB30_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991127.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     FKQYYASRENFLVNFYATNPDCALRPDYETIMENNTNKESLCLQSEKGGKTSYKDITK
                     IQLATTEKQKLLASLEVVNSMSSYVELLTVGMDVEDSKQIILLNQALDHANNAQKLL"
     gene            complement(646..1521)
                     /gene="rluB"
                     /locus_tag="ISB30_003529"
     CDS             complement(646..1521)
                     /gene="rluB"
                     /locus_tag="ISB30_003529"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAP1991128.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(1734..3629)
                     /locus_tag="ISB30_003530"
     CDS             complement(1734..3629)
                     /locus_tag="ISB30_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HAP1991129.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPNGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGISEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGDEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEAHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGEARKLDYALLGWAVSAN
                     LGREWAARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(3657..4277)
                     /locus_tag="ISB30_003531"
     CDS             complement(3657..4277)
                     /locus_tag="ISB30_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP1991130.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(4274..5155)
                     /gene="yciV"
                     /locus_tag="ISB30_003532"
     CDS             complement(4274..5155)
                     /gene="yciV"
                     /locus_tag="ISB30_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAP1991131.1"
                     /translation="MSDTNYSVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQQLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            5293..5337
                     /gene="trpL"
                     /locus_tag="ISB30_003533"
     CDS             5293..5337
                     /gene="trpL"
                     /locus_tag="ISB30_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAP1991132.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            5429..6991
                     /gene="trpE"
                     /locus_tag="ISB30_003534"
     CDS             5429..6991
                     /gene="trpE"
                     /locus_tag="ISB30_003534"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAP1991133.1"
                     /translation="MQTQKPTLELLTCVGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGVATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            6991..8586
                     /gene="trpD"
                     /locus_tag="ISB30_003535"
     CDS             6991..8586
                     /gene="trpD"
                     /locus_tag="ISB30_003535"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAP1991134.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEQTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRCVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            8590..9948
                     /gene="trpCF"
                     /locus_tag="ISB30_003536"
     CDS             8590..9948
                     /gene="trpCF"
                     /locus_tag="ISB30_003536"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAP1991135.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            9960..11153
                     /gene="trpB"
                     /locus_tag="ISB30_003537"
     CDS             9960..11153
                     /gene="trpB"
                     /locus_tag="ISB30_003537"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAP1991136.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            11153..11959
                     /gene="trpA"
                     /locus_tag="ISB30_003538"
     CDS             11153..11959
                     /gene="trpA"
                     /locus_tag="ISB30_003538"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAP1991137.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPVGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKATTRR"
     gene            complement(12010..12150)
                     /locus_tag="ISB30_003539"
                     /pseudo
     CDS             complement(12010..12150)
                     /locus_tag="ISB30_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
     gene            12335..12610
                     /locus_tag="ISB30_003540"
     CDS             12335..12610
                     /locus_tag="ISB30_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAP1991138.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            12607..13164
                     /locus_tag="ISB30_003541"
     CDS             12607..13164
                     /locus_tag="ISB30_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAP1991139.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(13576..13845)
                     /locus_tag="ISB30_003542"
     CDS             complement(13576..13845)
                     /locus_tag="ISB30_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP1991140.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            13902..>14083
                     /locus_tag="ISB30_003543"
     CDS             13902..>14083
                     /locus_tag="ISB30_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HAP1991141.1"
                     /translation="MVKSMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHVGNNHLDIG"
BASE COUNT         3530 a         3657 c         3446 g         3450 t
ORIGIN      
        1 agtagttttt gggcgttgtt tgcgtgatcg agcgcctgat taagtaatat aatttgttta
       61 ctatcctcaa catccatacc caccgtcaac aactccacat aggaactcat actgttaacg
      121 acctcaagtg aagccagtag tttttgtttt tctgttgttg ccaattgaat tttagtaatg
      181 tctttatacg atgttttgcc ccctttctct gattgcaaac ataaggactc tttattagta
      241 ttattttcca taatcgtttc gtaatcaggt cgcaatgcac aatccggatt agtcgcataa
      301 aaattaacaa gaaagttttc acgagaagca tagtattgtt taaagaaact tgcattcgcc
      361 gcaaccgcct tgttacccgc agtaataaca gtttcagctt gttcattctt caatgcttcg
      421 gttgcacaac ctgaaagcat taacagcgat agaaatattc cactatatct aatcataacc
      481 cacctcttga cgttgagttt atctcagata attaaatagt aatgtggcta ttccctggca
      541 ttattcataa gcaagtatga aattaaatta aaatactgcg actatttatc atacttcaaa
      601 tgaagaaaag gccccgcagg gcctttattt aatacttaac ttaaattaac cgttattatt
      661 acgtccgcca gaacgacgac cgccgctcac ctgactgtga cgtttcaccg cacgacgaat
      721 ctgattcgcc ttcatacgac gacggtcttt ttctacagcg actttagagc tggtttctgg
      781 cggtagttcc accagttcgc gcagatagtt agtctgggcg aggtccagct ccgtccagcc
      841 accgcgtggc agaccttttg gcagtgggat atcaccgtaa cgaacgcgaa tcaggcggct
      901 cacctgcaca ccaaccgctt cccacagacg acgaacttca cggttacggc cttcggtcag
      961 agtaacgttg taccactggt tgatcccttc gccgccgctg aacttgatgg ttttaaaggc
     1021 tgccggacca tcttccagct gcacgccacg actcaaatca cgcagtttcg cgtcgtcaac
     1081 ctgaccaaat acacgcacgg catattcacg ttcaacttca cggcttggat gcattaaacg
     1141 gtttgccagt tcaccgtcgg tggtgaacaa caacagacca caggtgttaa cgtccagacg
     1201 acctacggca atccagcgtg cgccacgcag ctttggcaga cggtcaaaca ccgttggacg
     1261 cccttccgga tcgttacggg tacacaactc accttccggt ttgtaatagg ccagaacgcg
     1321 acaaatttgt tcagcggact cacgtaccga aatcaggtga ccatcgatac ggattttcag
     1381 tccaggggta atttcaacgc gatcgccgag tttagcaatt ttgccatcaa cactcacacg
     1441 accggcttca ataatagatt cgatttcacg gcgagaaccg tggccggcac gcgccagcac
     1501 tttctgtagc ttttcgctca tagagcttcc ttttggtgtc gccttcccag gcgtctgata
     1561 tgcttttgtg caacaatgag tcagaactta catctgactc actgatagta aagtctgttt
     1621 gaactcttca ggccttaagt aaacgactcg acgttttaac tggcctggct ttgaggcgca
     1681 acatactaca ctatctttat ggttaaagct tgtttcgatt aacatcagct ttattaccag
     1741 ccaccaccgc ctccgccacc cgcaccgccg ccagcaccac caccggaata acttcgcccc
     1801 aacaaactgg tattggcccc cagactcaga tgcatctgta atgagaacaa aacgccacta
     1861 cgggcaattt ccggggcgcg gacagccgct gtgagtgatg gggtgatacg tgcagcccat
     1921 tctctgccaa ggtttgccga gaccgcccaa cctaacaaag cgtaatccag tttccgtgct
     1981 tcccctttcc tccgccgccc actgtgtgtt gctgcctctt tacgcgcaag atagttaacg
     2041 atagcttcac cgtgggcata acgttgctgc ccagtttggg taaactttgg cagaaaaata
     2101 taccccatgc cagtgagata accggtcagg aaaacagcgc taaaataacc cgctggcata
     2161 taccagtaaa agaatacatc tccgacattg atgtagagag aatagaaggc cacgcctgaa
     2221 caaatcagtg gtaaaaacaa agtgatgatt acacgattaa cgcaaggctt gtcatcatcg
     2281 cgggcaaaca aaagctcaag ggaacaaaat aagaaaggaa gaagccagca gatcataata
     2341 aacatatcgc ctaccagact cattcctgcc caacctacgg gactcaggtt accgtagaga
     2401 atgaccgcca atatcgccat cccgccccat tgcaaaaacg catcaggtcg gtaaaacgaa
     2461 gactttttac gcggcagata aaatttctgc atccgtaaaa atgccttgcg taagactctg
     2521 ttgcgtctcc ccgtaagaac gaccttatcg cctttacgaa ataacgcttc cagtaataac
     2581 tgctctgcgg gcagtaatac cgcattattt ctggtcgcgc cttcattaac acgaatcagg
     2641 atttgctggt tcttgtcata atgagcttcc agatgaattt ttcctttcac aatcagattt
     2701 acgatatcgg cgcaaaaacc tttatcgtca taaaccagct tcgcatcgag acgtaacatg
     2761 ccgggcgtgt aatctggcgg aatgacatcg gtttcaatca catctaccgg cgtaaattgc
     2821 ggcctgcgct tccagagata taaccatcca cacgccagga acaggcacgg aaaccaaatt
     2881 aacaacgatg acgtagaggg taaaagaaga tggctgaaaa tattcgtcgt ttgtggtgcc
     2941 ggggcattac caagtaaagc gtgaggccag cggtagagga ccgtgaaatc ttcacgataa
     3001 aacggatcgc gagattcaat tcttccgtcc tcaaggatgc ggccatttcg atatgtgtcg
     3061 ccctcatctc cggtaaagag atcgatttcg ctaaatggat tacccgcaat atcaggcaac
     3121 ttgagtgaaa atagggtctt atatatttca aacggccagt ggttaccggt cacgttccag
     3181 attagcagat cagaatcccc ctcacgcagg aaagcatttt taacctgata atgaatttga
     3241 taatgatagt cgccttgagg caagaaacgt tgtttatcgc ccacgacaat actcattaac
     3301 ccgatatgcc agtcaagatg ccagggttca gaaataccat cacgggtgac gccaacaata
     3361 tgatagttca gcagtgcagc gtccccatca ggctgcatcc agagtcgcgg taaatcacga
     3421 taaaatccgt gcttaatctg ctgatttcgc gcctgaaaat gaatgttctc tttgatgtcg
     3481 agcaccccat tcggttgcag agtggcttca acatcaaaga gcgagatccc ttcttcataa
     3541 gaagggatct ccgtgttttt cgcataactc aacgaagaga aaaaaagccc aactatcaat
     3601 agcaaaatgc agcgatatat ccctgccata atcttccatc cgtggaacgc aatttgttat
     3661 aagaaaggct tcacatcacc tacgccttca cgcaccacga ccggcgtatc atcggtgaga
     3721 tcaataaccg ttgttggttt ctgcccgaga tagccgccgt gaataatcaa atccacctgt
     3781 ttttccagac ggtctttaat ttcttccgga tcggattcgg taaattcact gcctggcagc
     3841 attagcgaag tggaaagcat cggttcgccc aatgcttcaa gcagcgcctg cgcaatcggg
     3901 ttagacggca cgcgcatacc gatggttttg cgtttttcct gcaacagacg gcgtggcacc
     3961 tccttagtcc ccttcaggat gaaggtatag ttgcctggcg tgttgttttt catcaaacga
     4021 aacgcaacgt tatcgacaaa tgaataggtc gagagttcag aaagatcgcg acacatcagg
     4081 gtaaagttgt gaccgtccgg cagctggcga atacgacaaa tacgctccat cgcgttttta
     4141 tcttcaattt tacagccgag cgcatagccg gaatcagttg gataaacaat caccccgcct
     4201 ttacgcacga tctccaccgc ctggttgatc aggcgttgct gcgggttatc aggatgaata
     4261 taaaaaaact ggctcataat tccctctctg tggtgttctg cggctgttcc catagctgcc
     4321 agacgccttc aacgcctgcg ggcagccaga gtttacgacc cagttcgatc cacgggcatg
     4381 gttgatgaaa atcagatcct tgtgatgccc ataaatgatg ctgacgcgca agtgtcgcca
     4441 gctgggtgcg ttcattgggc gattgctggc actgcgcgac ttccatcgcg tcgccgtggt
     4501 gttcggcaaa atgcgcaacc aatcttttca gccatttagc actaagattg taccgccctg
     4561 gatgagctag caccgcctta ccgccagaat gatgaatgac atcaatagct tgttctattg
     4621 tacaccactg tggcggaacg tatccggttt tcccgcgcgc cagatacttt ttaaagacat
     4681 ccgccattga actggctttt ccgcactcca caaggaaacg cgcaaaatga ccgcgcgtca
     4741 ccgcaccgcc ctgcgccagt tgttgcgcgc cttccagtgc cccaggtatt tgtgcttttt
     4801 caagtctttc ggcaatcagc tgcgcccgct gattgcgccg ttctgtctgc tgcgcgagga
     4861 actcacacat tagcggatga gtaatatcaa tattcagccc gacaatatga atttcatgat
     4921 tttcccagac cgtggaaatt tccacaccag gaataagatt cagcgccagt cccgaacgtg
     4981 aaatttcctc tcttgctggt gcaattgcag ctgtggtgtc atggtcggtg atcgccaggg
     5041 tgccgacgcg catctcgact gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg
     5101 aagctgtggt atggctgtgc aggtcgtaaa tcactgagta attcgtgtcg ctcaaggcgc
     5161 actcccgttc tggataatgt tttttgcgcc gacatcataa cggttctggc aaatattctg
     5221 aaatgagctg ttgacaatta atcatcgaac tagttaacta gtacgcaagt tcacgtaaaa
     5281 agggtatcga caatgaaagc aattttcgta ctgaaaggtt ggtggcgcac ttcctgaaac
     5341 gggcagtgta ttcaccatgc gtaaagcaat cagataccca gcccgcctaa tgagcgggct
     5401 tttttttgaa caaaattaga gaataacaat gcaaacacaa aaaccgactc tcgaactgct
     5461 aacctgcgta ggcgcttatc gcgacaaccc aaccgcgctt tttcaccagt tgtgtgggga
     5521 tcgtccggca acgctgctgc tggaatccgc agatatcgac agcaaagatg atttaaaaag
     5581 cctgctactg gtagacagtg cgctgcgcat tacagcatta ggtgacaccg tcacaatcca
     5641 ggcactttcc ggcaatggcg aagccctgct ggcactactg gataacgccc tgcctgcggg
     5701 tgtggaaaat gaacaatcac caaactgccg cgtactgcgc ttcccgcctg tcagtccact
     5761 gctggatgaa gacgcccgct tatgctccct ttcggttttt gacgcattcc gcttattaca
     5821 gaatctgttg aatgtaccga aggaagaacg agaagcaatg ttcttcggcg gcctgttctc
     5881 ttatgacctt gtggcgggat ttgaagattt accgcaactg tcagcggaaa ataactgccc
     5941 tgatttctgt ttttatctcg ctgaaacgct gatggtgatt gaccatcaga aaaaaagcac
     6001 ccgtattcag gccagcctgt ttgctccgaa tgaagaagaa aaacaacgtc tcactgctcg
     6061 ccttaacgaa ctacgtcagc aactgaccga agccgcgccg ccgctgccgg tggtttccgt
     6121 gccgcatatg cgttgtgaat gtaaccagag cgatgaagag ttcggtggcg tagtgcgttt
     6181 gttgcaaaaa gcgattcgcg ccggagaaat tttccaggtg gtgccatctc gccgtttctc
     6241 tctgccctgc ccgtcaccgc tggcggccta ttacgtgctg aaaaagagta atcccagccc
     6301 gtacatgttt tttatgcagg ataatgattt caccctgttt ggcgcgtcgc cggaaagttc
     6361 gctcaagtat gacgccacca gccgccagat tgagatttac ccgattgccg gaacacgtcc
     6421 acgcggtcgt cgtgccgatg gttcactgga tagagacctc gacagccgca tcgaactgga
     6481 gatgcgtacc gatcataaag agctttctga acatctgatg ctggtggatc tcgcccgtaa
     6541 tgacctggcg cgtatttgca cccccggcag ccgctacgtc gccgatctca ccaaagttga
     6601 ccgttactct tacgtgatgc acctggtctc ccgcgttgtt ggtgagctgc gccacgatct
     6661 cgacgccctg cacgcttacc gcgcctgtat gaatatgggg acgttaagcg gtgcgccgaa
     6721 agtacgcgct atgcagttaa ttgccgaagc agaaggtcgt cgccgcggca gctacggcgg
     6781 cgcggtaggt tattttaccg cgcatggcga tctcgacacc tgcattgtga tccgctcggc
     6841 gctggtagag aacggagtcg ccaccgtgca agccggtgct ggcgtggtac ttgattctgt
     6901 tccgcagtcg gaagccgacg aaacccgtaa taaagcccgc gctgtactgc gcgctattgc
     6961 caccgcgcat catgcacagg agacgttcta atggctgaca ttctgctgct cgataatatc
     7021 gactctttta cttacaacct ggcagatcag ttgcgcagca atggtcataa cgtggtgatt
     7081 taccgcaacc atattccggc gcagacctta attgaacgcc tggcgacgat gagcaatccg
     7141 gtgctgatgc tttctcctgg ccccggtgtg ccgagcgaag ccggttgtat gccggaactc
     7201 ctcacccgct tgcgtggcaa gctgccaatt attggcattt gcctcggaca tcaggcgatt
     7261 gtcgaagctt acgggggcta tgtcggtcag gcgggcgaaa ttcttcacgg taaagcgtcg
     7321 agcattgaac atgacggtca ggcgatgttt gccggattaa caaacccgct gccagtggcg
     7381 cgttatcact cgctggttgg cagtaatatt ccggccggtt taaccatcaa cgcccatttt
     7441 aatggcatgg tgatggcggt gcgccacgat gcggatcgcg tttgtggatt ccagttccat
     7501 ccggaatcca ttcttactac ccagggcgct cgcctgctgg aacaaacgct ggcctgggcg
     7561 cagcagaaac tagagcaaac caacacgctg caaccgattc tggaaaaact gtatcaggca
     7621 cagacgctta gccaacaaga aagccaccag ctgttttcag cggtggtacg tggcgagctg
     7681 aagccggaac aactggcggc ggcgctggtg agcatgaaaa ttcgcggtga gcacccgaac
     7741 gagatcgccg gggcagcaac cgcgctactg gaaaacgccg cgccgttccc gcgcccggat
     7801 tatctgtttg ccgatatcgt cggtactggc ggtgacggca gcaacagcat caatatttct
     7861 accgccagtg cgtttgtcgc cgcggcctgc gggctgaaag tggcgaaaca cggcaaccgt
     7921 tgcgtctcca gtaaatccgg ctcgtcggat ctgctggcgg cgttcggtat taatcttgat
     7981 atgaacgccg ataaatcgcg ccaggcgctg gatgaattag gcgtctgttt cctctttgca
     8041 ccgaaatatc acaccggatt ccgccatgca atgccggttc gccagcaact gaaaacccgc
     8101 acactgttca atgtgctggg gccattaatt aacccggcgc atcctccgct ggcgttaatt
     8161 ggtgtctaca gcccggagct ggtgctgccg attgccgaaa ccttgcgcgt gctggggtat
     8221 caacgcgcgg cagtggtgca cagcggcggg atggatgaag tttcattaca cgcgccgaca
     8281 atcgttgccg aactgcatga cggcgaaatt aagagctatc aattgaccgc tgaagatttt
     8341 ggcctgacac cctaccacca ggagcaactg gcaggcggaa caccggaaga aaaccgtgac
     8401 attttaacac gcttgttaca aggtaaaggc gacgccgctc acgaagcagc cgtcgcggcg
     8461 aatgtcgcca tgttaatgcg cctgcatggc catgaagatc tgcaagccaa tgcgcaaacc
     8521 gttcttgagg tactgcgcag tggttccgct tacgacagag tcaccgcact ggcggcacga
     8581 gggtaaataa tgcaaaccgt tttagcgaaa atcgtcgcag acaaggcgat ttgggtagaa
     8641 gcccgcaaac agcagcaacc gctggccagt tttcagaatg agattcagcc gagcacgcga
     8701 catttttatg atgcgctaca gggtgcacgc acggcgttta ttctggagtg caaaaaagcg
     8761 tcgccgtcaa aaggcgtgat ccgtgatgat ttcgatccgg cacgcattgc tgccatttat
     8821 aaacattacg cttcggcaat ttcagtgctg actgatgaga aatattttca ggggagcttt
     8881 gatttcctcc ccatcgtcag ccaaatcgcc ccgcagccga ttttatgtaa agacttcatt
     8941 atcgatcctt accagatcta tctggcgcgc tattaccagg ccgatgcctg cttattaatg
     9001 ctttcagtac tggatgacga acaatatcgc cagcttgcag ccgtcgccca cagtctggag
     9061 atgggtgtgc tgaccgaagt cagtaatgaa gaggaactgg agcgcgccat tgcattgggg
     9121 gcaaaggtcg ttggcatcaa caaccgcgat ctgcgcgatt tgtcgattga tctcaaccgt
     9181 acccgcgagc ttgcaccgaa actggggcac aacgtgacgg taatcagcga atccggcatc
     9241 aatacttacg ctcaggtgcg cgagttaagc cacttcgcta acggctttct gattggttcg
     9301 gcgttgatgg cccatgacga tttgaacgcc gctgtgcgtc gggtgttgct gggtgagaat
     9361 aaagtatgtg gcctgacacg tgggcaagat gctaaagcag cttatgacgc gggcgcgatt
     9421 tacggtgggt tgatttttgt tgcgacatca ccgcgttgcg tcaacgttga acaggcgcag
     9481 gaagtgatgg ctgcggcacc gttgcagtat gttggcgtgt tccgcaatca cgatattgcc
     9541 gatgtggtgg acaaagctaa ggtgttatcg ctggcggcag tgcaactgca tggtaatgaa
     9601 gatcagctgt atatcgacac tctgcgtgag gctctgccag cacacgtcgc catctggaag
     9661 gctttaagtg tcggtgaaac tcttcccgcg cgcgattttc agcacatcga taaatatgta
     9721 ttcgacaacg gtcagggcgg gagcggacaa cgtttcgact ggtcactatt aaatggtcaa
     9781 tcgcttggca acgttctgct ggcggggggc ttaggcgcag ataactgcgt ggaagcggca
     9841 caaaccggct gcgccgggct tgattttaat tctgctgtag agtcgcaacc gggtatcaaa
     9901 gacgcacgtc ttttggcctc ggttttccag acgctgcgcg catattaagg aaaggaacaa
     9961 tgacaacatt acttaacccc tattttggtg agtttggcgg catgtacgtg ccacaaatcc
    10021 tgatgcccgc tctgcgccag ctggaagaag cttttgtcag cgcgcaaaaa gatcctgaat
    10081 ttcaggctca gttcaacgac ctgctgaaaa actatgccgg gcgtccaacc gcgctgacca
    10141 aatgccagaa cattacagcc gggacgaaca ccacgctgta tctgaagcgc gaagatttgc
    10201 tgcacggcgg cgcgcataaa actaaccagg tactcggtca ggctttactg gcgaagcgga
    10261 tgggtaaaac tgaaattatt gccgaaaccg gtgccggtca gcatggcgtg gcgtcggccc
    10321 ttgccagcgc cctgctcggc ctgaaatgcc gtatttatat gggtgccaaa gacgttgaac
    10381 gccagtcgcc taacgttttc cggatgcgct taatgggtgc ggaagtgatc ccggtgcata
    10441 gcggttccgc gaccctgaaa gatgcctgta atgaggcgct acgcgactgg tccggcagtt
    10501 atgaaaccgc gcactatatg ctgggtaccg cagctggccc gcatccttac ccgaccattg
    10561 tgcgtgagtt tcagcggatg attggcgaag aaacgaaagc gcagattctg gaaagagaag
    10621 gtcgcctgcc ggatgccgtt atcgcctgtg ttggcggtgg ttcgaatgcc atcggtatgt
    10681 ttgcagattt catcaacgaa accgacgtcg gcctgattgg tgtggagcct ggcggtcacg
    10741 gtatcgaaac tggcgagcac ggcgcaccgt taaaacatgg tcgcgtgggc atctatttcg
    10801 gtatgaaagc gccgatgatg caaaccgaag acgggcaaat tgaagagtct tactccattt
    10861 ctgccgggct ggatttcccg tccgtcggcc cgcaacatgc gtatctcaac agcactggac
    10921 gcgctgatta cgtgtctatt accgacgatg aagccctgga agcctttaaa acgctttgcc
    10981 tgcatgaagg gatcatcccg gcgctggaat cctcccacgc cctggcccat gcgctgaaaa
    11041 tgatgcgcga aaatccggaa aaagagcagc tactggtggt taacctttcc ggtcgcggcg
    11101 ataaagacat cttcaccgtt cacgatattt tgaaagcacg aggggaaatc tgatggaacg
    11161 ctacgaatct ctgtttgccc agttgaagga gcgcaaagaa ggcgcattcg ttcctttcgt
    11221 caccctcggt gatccgggca ttgagcagtc gctgaaaatt atcgatacgc taattgaagc
    11281 cggtgctgac gcgctggagt taggcatccc cttctccgac ccactggcgg atggcccgac
    11341 gattcaaaac gccacactgc gtgcctttgc ggcgggtgtg accccggcgc agtgctttga
    11401 gatgctggca ctgattcgtc aaaaacatcc gaccattcct gttggcctgc tgatgtacgc
    11461 caacctggtg tttaacaaag gcattgatga gttttacgcc cagtgcgaga aagtcggcgt
    11521 cgattcggtg ctggttgccg atgtgccagt tgaagagtcc gcgcccttcc gccaggccgc
    11581 gttgcgtcat aatgtcgcac ctatctttat ttgcccgccg aatgccgacg atgatttgct
    11641 gcgccagata gcctcctacg gtcgtggata cacctattta ttgtcacgtg cgggcgtgac
    11701 cggcgcagaa aaccgcgccg cgttacccct caatcatctg gttgcgaagc tgaaagagta
    11761 caacgctgca cctccattgc agggatttgg tatttccgcc cctgatcagg taaaagcagc
    11821 gattgatgca ggagctgcgg gcgcgatttc tggttcggcc atcgttaaaa tcatcgagca
    11881 acatattaat gagccagaga aaatgctggc ggcactgaaa gcttttgtac aaccgatgaa
    11941 agccactaca cgacgttaat ttgagacaaa ccgccagaaa acatctggcg gtttattgat
    12001 ggtaaaaaat tagaagcggt aacctgcaga gaacataaat acccacgggt caaggcgtac
    12061 gttgtcagaa taacgccctg caggcagcac tgcgccgttg gatttatatt tcacttcggt
    12121 atcgatatcc atgtaccaga ccgacatgtt ttaaaaaatg caataagaca ttgtatctga
    12181 aagaaaaaac aggatttgag gcgctttttt ataccgtttt tccgtgtttt taactgcctg
    12241 attttattga aatctttttt agttttgcag aaaggatttt ttgctcgatc gctttaattt
    12301 tatacattcc tgataacgga aatttttttg cccgttgaca gtttttgttc tccggggcta
    12361 tattcctcac acaaccgcaa aatcggttgt cgggattggc gtctcggata acatcacaac
    12421 gcatagccgc gtttgcggtt ttttttgcgt taagcattgc cacattcgca ttatggtggg
    12481 gcgtgcaggg gcggagaaat ccgcgccggg ttttgtgatg accggttacg ccaaccctgt
    12541 acgtctcacc acctccctga ttggcgtcag aggtggtgat catccaatta attggagtca
    12601 tcacatatga atacagctat aactttttct aacgagtcac caaaagtctt tttcaaagat
    12661 ggtcgcctgg ttaccacctc tcaagctgtt gccgactact tcgataaaca acataaacac
    12721 gttttagcta agattgacac actcgactgc tctcccgaat ttacatcagc caacttttcg
    12781 gccgatgttc agactgtcga aattggcaac ggcgcagaac gtgaatcacg atgctacctg
    12841 atcacaaaag acggcttcat gttcctcgta atgggattta ctggcaaaaa agcagccaga
    12901 ctaaaagaag cttacattga aaaatttaac gcaatggaag aagaactcca caaacgtcct
    12961 cctgcagcac aaaactcccc cgccccaaat aatggatgcg cattactgat ccacttcgat
    13021 aaacacggtc aggtcgagtt cacagaaaaa gtccccgccg atgcgatggt atgcacgctg
    13081 gaacggttta aattttatct ggagcaacat gggtggatcg ttgcccgtaa agaacaattg
    13141 gtggaaaggt tgatgcggtt ttaatgaatg caaaaccccg gatgatcacc ggggttaatt
    13201 ttatcgagcg tgattgccgc taatcgccat cgctctgatt gagcgtaaat cattcaggtt
    13261 attcagttct tccttctgct ctcgctgacg tcaataaatc ccatcattgc gatcaacctc
    13321 atccttaacc attgatgcga gcagttcttc cagtttctgc gctgacagct ttacctggtg
    13381 atcttcagca tcttcccggg ctattgtggt tcgcgcagtg gctgattttg ctgacatcac
    13441 cacagggggc agacggacca ttgaaccatg gcctccggaa caaataaggg caaaaataac
    13501 aaccactatc gaaagctcac aaactaaccg cagcacgttc ctgcatacga cgtgtctgcg
    13561 gcataatccc aatgattact ccctgacagg atttgcaggc cactcaatat caggtgcagt
    13621 tgatgtatca acacgattca acaatacccg atatttattc catgcctcca gcaacgatct
    13681 ttcttcctcc gttgcgattt ccagatctac agcatcctgc agtggcgcaa tatactcact
    13741 gaattcctgg atgtagaact gtgtggtgac ggtcttccag ccattcggct cctgctgtat
    13801 cgaagcatac caggctattt caatatcgct atgctgcggc agcatttaac cccttgtaat
    13861 tcatcgccat aattgattta attcacaaat aaaactataa catggtgaaa tcaatgaaaa
    13921 aaaacacaga tgatggggct aaaatttaca caccacttac cctaaagctt tatgactggt
    13981 gggttttggg agtatcaaat cggcttgcat ggggatgtcc tacaaaggaa caccttcttc
    14041 cacactttct ggaacatgta ggtaacaacc atctggatat tgg
//
LOCUS       DACADF010000122        13950 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.124, whole genome shotgun sequence.
ACCESSION   DACADF010000122 DACADF010000000
VERSION     DACADF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.124"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            195..473
                     /locus_tag="ISB30_003544"
                     /pseudo
     CDS             195..473
                     /locus_tag="ISB30_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(524..769)
                     /locus_tag="ISB30_003545"
                     /pseudo
     CDS             complement(524..769)
                     /locus_tag="ISB30_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(1142..1372)
                     /locus_tag="ISB30_003546"
                     /pseudo
     CDS             complement(1142..1372)
                     /locus_tag="ISB30_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2111..2557)
                     /locus_tag="ISB30_003547"
                     /pseudo
     CDS             complement(2111..2557)
                     /locus_tag="ISB30_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155169.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(2744..2828)
                     /locus_tag="ISB30_003548"
     tRNA            complement(2744..2828)
                     /locus_tag="ISB30_003548"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2792..2794),aa:Leu,seq:caa)
     gene            3024..4043
                     /gene="ahr"
                     /locus_tag="ISB30_003549"
     CDS             3024..4043
                     /gene="ahr"
                     /locus_tag="ISB30_003549"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAP1991142.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVI
                     LKADF"
     gene            4173..5675
                     /locus_tag="ISB30_003550"
     CDS             4173..5675
                     /locus_tag="ISB30_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAP1991143.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLDIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRTFTPKDQKAV
                     KAAAQTMRVNPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPMTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASAEQQNNPAVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5836..6918)
                     /gene="lptG"
                     /locus_tag="ISB30_003551"
     CDS             complement(5836..6918)
                     /gene="lptG"
                     /locus_tag="ISB30_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAP1991144.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQIDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6918..8018)
                     /gene="lptF"
                     /locus_tag="ISB30_003552"
     CDS             complement(6918..8018)
                     /gene="lptF"
                     /locus_tag="ISB30_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAP1991145.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            8285..9796
                     /gene="pepA"
                     /locus_tag="ISB30_003553"
     CDS             8285..9796
                     /gene="pepA"
                     /locus_tag="ISB30_003553"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAP1991146.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            10152..10595
                     /gene="holC"
                     /locus_tag="ISB30_003554"
     CDS             10152..10595
                     /gene="holC"
                     /locus_tag="ISB30_003554"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAP1991147.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10595..13450
                     /gene="valS"
                     /locus_tag="ISB30_003555"
     CDS             10595..13450
                     /gene="valS"
                     /locus_tag="ISB30_003555"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP1991148.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(13504..>13950)
                     /locus_tag="ISB30_003556"
     CDS             complement(13504..>13950)
                     /locus_tag="ISB30_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HAP1991149.1"
                     /translation="QILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVST
                     SYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIW
                     MIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT         3392 a         3628 c         3746 g         3184 t
ORIGIN      
        1 tgcataatgt atgcgccccc ctatcaggct gtctcttata cacaaatccc cacccgggga
       61 tttgtgatgt ctgtcagtct gctttagggg ggactctttc cattcggtag tttccttcct
      121 ccccggcagc gattttttga actttttctt cacccgttga tcttctctta tatttcattt
      181 tcttcgggtc tgttatggta ctttctgatt gttattcatg ggataacgag cagtttggac
      241 atgcccgtct cggtgatccg cgtcgtacac gacgccttgt gtcactggcc tcttctctcg
      301 ctcagcatgc aggtctttct attgtgaaat catcccactc cactgctcag gttgaaagtg
      361 cgtaccgcct gatacgtaac ccgtctgtgt ccccggaagc cattgcctaa gccggattta
      421 ccgccactgc aagggcatgt gaggctcatc cgctgctcct cgcactggaa gacaccacca
      481 ctaatcgtaa gcgtacagcg agggccgtat tgacggggat gtgttattca gctggcagtg
      541 ctatacgcca cggaagcagt tcgctgaccc ggttgaccgg ccagtctgct atgacgccaa
      601 gcacatggcg aaggtagctt tctggatcca cgtcattcag tttgcacgtc ccgatcaggc
      661 tgtacagtag cgctccccgc tcaccaccat ggtcagagcc gaagaacagg aagtttttac
      721 gacccagact gaccgtcaac gccacaatct ttgactgttt gacattgtca tgcccgcagg
      781 ccagcgaatc taaagacatc agactgcttg catcagccag ttgcgtatct ggctatgtcc
      841 cttaattggt aagggttcga ggaactcaac attaacgcat ttttccgccc atcaccggca
      901 gtgtatatga acgattgcac tcttacgatg gttgatttat ttggttgcgt aggggctagc
      961 tagtctggta ccggaggctg gatctgaagt actgacccca agaataacgc cgggcagact
     1021 catattctga cccattgtca gcccgcgtgt ttactgctct agctcatttc ctgttgtcag
     1081 gcgttcaatt cccaactgca ggttatggcc cggtgcacaa acacctgacg gcaggtatca
     1141 cttacccaaa gaagataaag ccctcaagag gcagtaactc tgccaacctc tcctccggcc
     1201 actccgggca gacgcgttag gacgtccgtc agccaagcat gtggctccag actgctgttt
     1261 ccagaaggct cattatctgt gccgctcggt tcccggccat cagtgaacca gcgaacaacc
     1321 aggcctttcg tcccataacg accggacgga tagcacgctc gcagatattg ttgtgtgtgc
     1381 cttgaaatgc acgatctttc cgtcggtgaa agtccgcccc gactaactct cgctccaact
     1441 ggaaggacag gaagcggtcc aggaggtaac aaaagggctg aatctctccc tgaaacgact
     1501 tcttcaggaa gtcaatgggt tatgcgggcc gtaacgtgag tgaacactga gcagccatcg
     1561 aaatgccgtc gatggtgccg atacgccaca ataacgggga aggccgacga tgaccgggaa
     1621 gagagtgaga aaggcatcgg tcatacccac cggggtagtg gtgtcagcat gcataacaat
     1681 tgatcgatcg tgcaacaaga gaagtccata acgatgcctg tccgggtgac gggcaacaac
     1741 gcctcccgag ggggtgaatc ggttgttatg gatggcagat ggggtgtagt agtgaaaata
     1801 ctgagtaatg tcataggagc gaaggccccc tgttgcagta ccagtgtaac atgcaggaag
     1861 agcccgggga ttgccatcag cctcacaacc ccgctcgaac cggttcagaa cctacagaaa
     1921 atactacagg agaaagcaaa gtgaacctcc ccgtgtttcc tgtttatcat tttctggtca
     1981 gtgccgcgat tctggtattc gtggtgattt tctggcggac acaccatcgc gaccaccgta
     2041 actggctggc tctacggcta ttcgtattat gctctgtaaa ccgttggcca ttgcggatgg
     2101 ttaaaggaac aataggtgct aataaatctc gagtttttaa ctcagcgata ttacgttcac
     2161 caagcgctgc aaaaagatta tcttcaagac tttttagcac acgatgggcg tgatcttcag
     2221 accacttttt attggtgcca tgccactcaa tcgcgacttc tttaaaggtt cgtgctttac
     2281 tctgttcaac cttatcattt ttctttttgt ctcccggatc gacgccattc gcaagcagct
     2341 tacgcgcctc gtcacgacgt actctggcat ccgctagtgt gatttcagga taaaccccaa
     2401 gtgccagcat tttttgcttt ccctcataac ggtactgcaa acgccagtac ttagaaccat
     2461 ttggatggac aagcagatgc ataccgccac catcagtcag cttatattgc ttatccgttg
     2521 gcttggctgc tctgactttg atatctgtta atgccatgcg tattccctcc ctgttggtac
     2581 aagcattatc gaaccggaaa taccatcatc tgtaccaaca tatgttggta gatgtacgtt
     2641 gatatcggtt gaccttgaga gagttaatat agcgggaaag atagataaac actggatttc
     2701 aggcataaaa aaagacctca gttgaggtct atttacatac ttttggtgcc gaaggccgga
     2761 ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac cgatttcgcc
     2821 acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg cacgccacca
     2881 tgcaagcgcc agcttgctcg cacctcgcta agtgctgaaa ttttcagcat tgcatacagc
     2941 gatgtgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac acttcccaga
     3001 caacaccaga gaaggaccaa aaaatgtcga tgataaaaag ctatgccgca aaagaagcag
     3061 gcggcgaact ggaagtttat gagtacgatc ccggtgaact gaagccacaa gatgttgaag
     3121 tgcaggtgga ttactgcggg atctgccatt ccgatctgtc gatgatcgat aacgaatggg
     3181 gattttcaca atatccgctg gttgccgggc atgaggtgat tggtcgcgtg gtggcgctcg
     3241 ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt ggctggacgg
     3301 cgcgtagctg cgggcattgc gatgcctgta ttagcggtaa tcagatcaac tgtgagcaag
     3361 gtgcggtgcc gacgattatg aatcgcggtg gctttgccga gaagttgcgt gcggactggc
     3421 aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg ctgttgtgcg
     3481 gtggtatcac ggtcttcaaa ccactgttaa tgcaccatat cactgccacc agccgcgttg
     3541 gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac gcaatgggat
     3601 gcgaggtgac agcctttagt tcgaatccgg cgaaagagca ggaagtactg gcgatgggtg
     3661 ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agcactggcg gggcagtttg
     3721 atctcattat caacaccgtc aacgtcagcc tcgactggca gccttatttt gaagcgctga
     3781 cgtacggcgg taatttccat acggtcggtg cggttctcac gccgctgtct gttccggcct
     3841 ttacgttaat tgcgggcgac cgcagcgtct ctggttctgc taccggcacg ccttatgagt
     3901 tgcgtaagct gatgcgcttt gccgcccgca gcaaggttgc gccgactacc gaattgttcc
     3961 cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg cgctaccgag
     4021 tgatcctgaa agccgacttc tgatcccttt gccgggtgcc tgtcgcccgg cacttaaatc
     4081 tctttggaag gcgtctcgca atctgctgaa tttagcggtg aagagtgcgc agccgttgcc
     4141 tatactccag gcaggaaact ggaggaaatc tcatgagcga acccctgtta attgcccgca
     4201 cgccggacac agaactattt ttactgccgg gaatggctaa ccgtcacggg ctgattactg
     4261 gcgcaacggg gacgggtaaa actgtcacgc tgcaaaaact ggcggagtct ctatcggaaa
     4321 tcggtgttcc ggtgtttatg gccgatgtga aaggcgatct gaccggcatc gcgcaggcag
     4381 gaacggcgtc ggaaaaactg ctcgcaaggc ttaaaaatat cggcgtcaat gactggcaac
     4441 cgcatgccaa tccggtggtg gtgtgggata tctttggcga gaaaggccat ccggtgcggg
     4501 cgacggtttc ggatctgggg ccgctgttgt tggcgcggct gttgaatctc aacgatgtgc
     4561 agtctggcgt gctggatatc atcttccgca ttgctgacga tcaggggctg ttactgctcg
     4621 actttaaaga tttgcgggcg attacccagt acatcggcga taacgccaaa tctttccaga
     4681 atcagtacgg aaatatcagt agcgcatcgg ttggtgccat ccagcgcggg ctgttgtcgc
     4741 tggaacagca aggcgcagca cacttctttg gtgagccgat gctggatatc aaagactgga
     4801 tgcgcaccga tgccaacggt aaaggcgtta tcaatatcct cagcgccgag aagctttatc
     4861 agatgccgaa actgtatgcc gccagcctgt tgtggatgct ctcggagttg tatgaacaat
     4921 tgccggaagc gggcgatctg gagaaaccaa aactggtgtt tttcttcgac gaggcacatc
     4981 tgctgtttaa cgatgcaccg caggtactgc tggataagat tgagcaggtg ataaggctta
     5041 ttcgctcaaa aggcgtgggc gtctggttcg tttcgcaaaa tccgtccgat attccggata
     5101 acgtgctcgg ccagctcggt aatcgcgttc aacacgcttt gcggactttt acgcccaaag
     5161 atcagaaagc ggtaaaagct gcggcgcaaa ccatgcgggt caatccggca tttgataccg
     5221 aaaaggcgat tcaggaactg ggcaccggcg aggcgttgat ctcttttctg gatgcgaaag
     5281 gaagcccttc tgtggtggaa cgggcgatgg tgattgcgcc ttgttcgcgg atgggaccga
     5341 tgacggaaga tgagcgtaat ggcttgatta atcactctcc ggtgtatggc aagtacgagg
     5401 atgacgtgga ccgggaatcc gcctatgaga tgttgcaaaa aggcgtacag gcaagcgccg
     5461 agcagcaaaa taatcctgcc gtgaaaggga aagaggtggc ggtggatgac ggcattcttg
     5521 gtggattgaa ggatattttg tttggcacta ccggaccacg cggcgggaag aaagatggtg
     5581 tggtgcaaac aatggcgaaa agcgccgccc gccaggtgac gaatcagatt gtacgtggga
     5641 tgttggggag tttgctgggg ggaagaagaa ggtaagtcag gcgcgatttg tctttgcctg
     5701 atgcgacgct ggcgcgtctt atcatgccta caccactgca atatattgga ttttcatgat
     5761 ttttcaggca tgaacgaagc acactttgct aacaataata aagggagctt tcgctccctt
     5821 tattcgttca ttcggttacg attttctcat taacagccac aggctgatta agaagaagct
     5881 ggcgcttggc aacagtgcgc cgatgatcgg cgggatgcca taaaccaacg tcagcgggcc
     5941 gaagatctgg tccagtacat agaagacaaa accgaagctg ataccggtga ccacacgcac
     6001 acccatcggt acgctacgca gtgggccaaa gatgaacgac agcgccatca gcatcatcac
     6061 cgccacggat agcggctgga agattttgct ccacatgttg agctgataac gtccggcatc
     6121 ctgaccgctc gatttcagat acttcacata gttgtgcaaa ccgctgatag agagtgcatc
     6181 cggatccagc gccaccacgc ccagtttgtc aggcgtgagg ttggttttcc aggtgccgct
     6241 caccgtctgc gaaccggtga tctgtttcgg attggtcaga tcagattcat caatctgcga
     6301 cagacgccag actttatgtt ccgggtcaaa cttcgcggta gcggcatagc gtacggattg
     6361 cagacgacga ttctcgttaa aggcataaat actgatgcca cctaactctt cgtcaccttt
     6421 aacccgctca atgtagacga agttgttgcc atctttcgcc cataaacctt gctgggtaga
     6481 gagcaacgag ccgccgtaca tcgcctgcgc acggtagtta cgcgccatct gctcgccctg
     6541 cggcgcgacc cattcgccaa tcgccatcgt cagcaagacc agcggaatgg cggttttcat
     6601 caccgacagc gccacctgca tacgggtaaa accagaagcc tgcatcacca ccagttcgct
     6661 gcgctgcgcc agcatcccaa gaccaagcaa cgccccaagc aaagccgcca tcgggaagaa
     6721 tatttgcaca tctttcggca cgctcagcaa ggtatacatt cctgcgccta acgcgtcgta
     6781 gctcccctgc ccggcttttt tcaactgatc gacaaatttg ataatgcccg acagcgacac
     6841 cagcatgaac agcgtcatca tgatggtggt gaaaatagtt ttaccgatat agcggtcaag
     6901 tacgccaaaa ggttgcatca caccgctcct ttacgcgaaa aactggcgcg caggcggcgg
     6961 accggcacag tgtcccaaag gttgagaaca atcgccaaag ccagataaat caggttaacg
     7021 gtccacatcc acagcgtcgg gtccagctta cctttaccgc cgttcgattt cagggaggtc
     7081 tggatcagga agaaaagtag atacagcagc atggctggca gcatcgacag tacgcgtccc
     7141 tgacgcgggt taaccacgct caacggtaca accataagtg ccatcataaa cacggtgaat
     7201 accaacgtga tacgccagtt cagttctgcg cgggcacgat cggtgtcagt gttccacaat
     7261 gtgcgcatgt ccatctggtc ggtatcgttc gggtcgagcg ccaccgcctg gtgaccaatg
     7321 atcgcctgat aatcctggaa atccgtaatg cggaaatcac gtaacagtgc agtgccttcg
     7381 aagcgcgttc cctggttgag agtgacgacc tgggagccgt cgcgcagctg ggttaaatgt
     7441 ccggaatcgg ccaccaccac agaaggtcgt gcattacctt ttggtcgaat ttgtgcgagg
     7501 aacacatctt tgaaatcgct gccgtcaacg ctttcgatga acagcaccga gctgccatta
     7561 gtcgcttgct ggaattgccc ttgcgccagc gccgccatgc cagggttcgc tttcgcttct
     7621 gctaacactt catcctgatg acgcgatgac cacggtcccg cccacatcac gttaaccgcc
     7681 gcgacgattg ccgtgaatac cgcaaggatc attgccgctt tcaccagaac cgctttgctc
     7741 aggccgcagg catgcattac cgtaatttca ctttcggtat acagtttgcc cagcgtcatc
     7801 agcagcccga ggaacaggct taatggcagg ataagctgcg ccatttccgg cacgcccaac
     7861 ccgagaaggg agagcaccag attcgccgga atatcgccgt caaccgctgc gccgaggatc
     7921 ctcactaact tttgacagaa gaagatcaaa agcaagatga agagtatcgc cagctggctt
     7981 ttgagcgtct cccgcaccag atatcttatg attatcactt taaatacgcc cgtaaaaact
     8041 cgtcttttgc aggattttag cttgtttcat ggcttaaacg tcatttattc tcttgagtcg
     8101 tcgaaatcgt cgctaagata attatactca acggattcac ctctcagatt ttgttctgac
     8161 gtgccaatgc cgtaataacg ttaagattaa cacgaagtca tcgcaacagc ggacatgagt
     8221 tacgaaagct tgcaattcta tctgtagcca ccgccgttgt ctttaagatt caggagcgta
     8281 gtgcatggag tttagtgtaa aaagcggtag cccggagaaa cagcggagtg cctgcatcgt
     8341 cgtgggcgtc ttcgaaccac gtcgcctttc tccgattgca gaacagctcg ataaaatcag
     8401 cgatgggtac atcagcgccc tgctacgtcg gggcgaactg gaaggaaaac cggggcagac
     8461 attgttgctg caccatgtac caaacgtgct ttctgaacgc atcctcctta ttggttgcgg
     8521 caaagaacgt gagctggatg agcgtcagta caagcaggtt attcagaaaa ccattaatac
     8581 gctgaatgat actggctcaa tggaagcggt ctgctttctg actgaactgc acgttaaagg
     8641 ccgtaacaac tactggaaag tgcgtcaggc tgtcgagacg gcaaaagaga cgctctacag
     8701 tttcgatcag ctgaaaacga acaagagcga accgcgtcgt ccgctgcgta aaatggtgtt
     8761 caacgtgccg acccgccgtg aactgaccag cggtgagcgc gcgatccagc acggtctggc
     8821 gattgccgcc gggattaaag cagcaaagga tcttggcaat atgccgccga atatctgtaa
     8881 cgccgcttac ctcgcttcac aagcgcgcca gctggctgac agctacagca agaatgttat
     8941 cacccgcgtt atcggcgaac agcagatgaa agagctgggg atgcattcct atctggcggt
     9001 cggtcagggt tcgcaaaacg aatcgctgat gtcggtgatt gagtacaaag gcaacgcgtc
     9061 ggaagatgca cgcccaatcg tgctggtggg taaaggttta accttcgact ccggcggtat
     9121 ctcgatcaag ccttcagaag gcatggatga gatgaagtac gatatgtgcg gtgcggcagc
     9181 ggtttacggt gtgatgcgta tggtcgcgga gctacaactg ccgattaacg ttatcggcgt
     9241 gttggcaggc tgcgaaaaca tgcctggcgg acgagcctat cgtccgggcg atgtgttaac
     9301 caccatgtcc ggccaaaccg ttgaagtgct gaatactgac gctgaaggcc gcctggtact
     9361 gtgcgacgtg ttaacttacg ttgagcgttt tgagccggaa gcggtgattg acgtggcgac
     9421 gctgaccggt gcctgcgtga tcgcgctggg tcatcatatt actggtctga tggcgaacca
     9481 taatccgctg gcccatgaac tgattgccgc gtctgaacaa tccggtgacc gcgcatggcg
     9541 cttaccgctg ggtgacgagt atcaggaaca actggagtcc aattttgccg atatggcgaa
     9601 cattggcggt cgtcctggtg gggcgattac cgcaggttgc ttcctgtcac gctttacccg
     9661 taagtacaac tgggcgcacc tggatatcgc cggtaccgcc tggcgttctg gtaaagcaaa
     9721 aggcgcaacc ggtcgcccgg tggcgttgct ggcacagttc ctgttaaacc gcgctgggtt
     9781 taacggcgaa gagtaattgc gtcaggcaag gctgttattg ccggatgcgg cgtgaacgcc
     9841 ttatccgacc tacacagcac tgaactcgta ggcctgataa gacgcgcaag cgtcgcatca
     9901 ggcaatgctg ttattgtcgg atgcggcatg aacgccttat ccgacctaca cacagcactg
     9961 aactcgtagg cctgataaga cacaacagcg tcgcatcagg caatgctgtt attgtcggat
    10021 gcggcgtgag cgccttatcc gacctacaca cagcactgaa ctcgtaggcc tgataagaca
    10081 caacagcgtc gcatcaggcg ctgcggtgta tacctgatgc gtatttaaat ccaccacaag
    10141 aagccccatt tatgaaaaac gcgacgttct accttctgga caatgacacc accgtcgatg
    10201 gcttaagcgc cgttgagcaa ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta
    10261 agcgcgtgct catcgcctgt gaagatgaaa agcaggctta ccggctggat gaagccctgt
    10321 gggcgcgtcc ggcagaaagc tttgttccgc ataatttagc gggagaagga ccgcgcggcg
    10381 gtgcgccggt ggagatcgcc tggccgcaaa aacgcagcag cagcccgcgc gatatattga
    10441 ttagtctgcg aacaagcttt gcagattttg ccaccgcttt cacagaagtg gtagacttcg
    10501 ttccttatga agattctctg aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg
    10561 ctggtttcaa cctgaatacg gcaacctgga aataatggaa aagacatata acccacaaga
    10621 tatcgaacag ccgctttacg agcactggga aaagcagggc tactttaagc ctaatggcga
    10681 tgaaagccag gaaagtttct gcatcatgat cccgccgccg aacgtcaccg gcagtttgca
    10741 tatgggtcac gccttccagc aaaccatcat ggataccatg atccgctatc agcgcatgca
    10801 gggcaaaaac accctgtggc aggtcggtac tgaccacgcc gggatcgcta cccagatggt
    10861 cgttgagcgc aagattgccg cagaagaagg taaaacccgt cacgactacg gccgcgaagc
    10921 tttcatcgac aaaatctggg aatggaaagc ggaatccggc ggcacgatta cccgtcagat
    10981 gcgccgtctc ggcaactccg tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct
    11041 gtccaatgcg gtgaaagaag ttttcgttcg tctgtataaa gaagacctga tttaccgtgg
    11101 caaacgcctg gtaaactggg atccgaaact gcgcaccgct atctctgacc tggaagtgga
    11161 aaaccgcgaa tcgaaaggtt cgatgtggca catccgctat ccgctggctg acggcgcgaa
    11221 aaccgcagac ggtaaagatt atctggtggt tgcgactacc cgtccggaaa ccctgttggg
    11281 cgataccggc gtggccgtta acccggaaga tccgcgttac aaagacctga ttggcaaata
    11341 tgtcattcta ccgctggtta accgtcgtat tccgatcgtt ggcgacgaac acgccgacat
    11401 ggaaaaaggc accggctgcg tgaaaatcac tccggcgcac gactttaacg actatgaagt
    11461 gggtaaacgt cacgcgctgc cgatgatcaa catcctgacc tttgacggcg atatccgtga
    11521 aagcgcccag gtgttcgata ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc
    11581 ggcagagttc cagaaactcg agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga
    11641 cgcactcggc ctgctggaag aaattaaacc gcacgacctg accgttcctt acggcgaccg
    11701 tggcggcgta gttatcgaac caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact
    11761 ggcgaaaccg gcggttgaag cggttgagaa cggcgacatt cagttcgtac cgaagcagta
    11821 cgaaaacatg tacttctcct ggatgcgcga tattcaggac tggtgtatct ctcgtcagct
    11881 gtggtggggt caccgtatcc ctgcatggta cgacgaagca ggtaacgttt atgttggccg
    11941 caacgaagaa gaagtgcgca aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga
    12001 cgaagacgtt ctcgacacct ggttctcttc tgcactgtgg accttctcta cccttggctg
    12061 gccggaaaac accgacgccc tgcgtcagtt ccacccaacc agcgtgatgg tatccggctt
    12121 cgacatcatc ttcttctgga ttgcccgcat gatcatgatg accatgcact tcatcaaaga
    12181 tgaaaatggc aaaccgcagg tgccgttcca cactgtttac atgaccggtc tgattcgtga
    12241 cgacgaaggc cagaagatgt ccaaatccaa gggtaacgtt atcgacccgc tggatatggt
    12301 tgacggtatt tcgctgccag aactgctgga aaaacgtacc ggcaatatga tgcagccgca
    12361 gctggcggac aaaatccgta agcgcaccga gaaacagttc ccgaacggca ttgagccgca
    12421 cggcaccgat gccctgcgct tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa
    12481 ctgggatatg aagcgcctgg aaggttaccg taacttctgt aacaagctgt ggaacgccag
    12541 ccgctttgtg ctgatgaaca cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac
    12601 gctgtcgctg gcggaccgct ggattctggc agagttcaac cagaccatca aagcgtaccg
    12661 cgaagcgctg gacagcttcc gcttcgatat cgctgcaggc attctgtatg agttcacctg
    12721 gaaccagttc tgcgactggt atctggagct gaccaagccg gtaatgaacg gtggaaccga
    12781 agccgaactg cgcggtactc gccatacgct ggtgactgta ctggaaggtc tgctgcgcct
    12841 cgcgcatccg atcattccgt tcatcaccga aaccatctgg cagcgtgtga aagtactttg
    12901 cggtatcact gccgacacca tcatgctgca gccgttcccg cagtacgatg catctcaggt
    12961 tgatgaagcg gcgctggccg acaccgagtg gctgaagcag gcgatcgttg cggtacgtaa
    13021 catccgtgca gaaatgaaca tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg
    13081 cagcgcggat gcagaacgtc gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg
    13141 tctggaaagc atcaccgtgc tgcctgccga tgacaaaggt ccggtttccg ttacgaagat
    13201 tatcgacggc gcagagctgc tgatcccgat ggctggcctc atcaacaaag aagatgagct
    13261 ggcgcgtctg gcgaaagaag tggcgaagat cgaaggcgaa atcagccgca tcgagaacaa
    13321 gctggcgaat gaaggctttg tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga
    13381 gaagctggaa ggctatgcgg aagcgaaagc gaagctgatt gaacagcagg ctgttatcgc
    13441 cgcgctgtaa tcaacaccgt gatgaaaacg aaggccggag catgctccgg ccttttttat
    13501 ctcttacaga aatggtaaag aaggcataat tccccgtgaa atactggcta agaagccttt
    13561 atctggttgt tcttctttat tgattaaagg aaggtcatcc aaatcgccca gcaaatacgt
    13621 attttttgcc tgccagtcaa tcatccatat tttcagcagt ggataagcca gaccgcccgt
    13681 aatcccggat atcaccacca acgcacacat gcgataaagc ataccgtggt atgttaaagt
    13741 tgagcgaaaa cgaatacgcc catcattcag tgacaggttg ctcataaaat gatttcgcaa
    13801 cgacaccgtt aaataactgg tgctaacagc aatcccaaaa taatagatta actgcgcgat
    13861 tatcatttta cgttgcattt ccataaattg ctgtaaattc tcaatatcat catttgcata
    13921 cacagaacta tcatacgcat ttaatatttg
//
LOCUS       DACADF010000123        13669 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.125, whole genome shotgun sequence.
ACCESSION   DACADF010000123 DACADF010000000
VERSION     DACADF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.125"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(149..838)
                     /locus_tag="ISB30_003557"
     CDS             complement(149..838)
                     /locus_tag="ISB30_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAP1991150.1"
                     /translation="MKKIITLMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(949..1653)
                     /locus_tag="ISB30_003558"
     CDS             complement(949..1653)
                     /locus_tag="ISB30_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1991151.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCGKST
                     ILRVIAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDAARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            complement(1640..2476)
                     /locus_tag="ISB30_003559"
     CDS             complement(1640..2476)
                     /locus_tag="ISB30_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1991152.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            complement(2469..3302)
                     /locus_tag="ISB30_003560"
     CDS             complement(2469..3302)
                     /locus_tag="ISB30_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1991153.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDIVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMIADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            complement(3302..4351)
                     /locus_tag="ISB30_003561"
     CDS             complement(3302..4351)
                     /locus_tag="ISB30_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1991154.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTGQPV
                     LQDLLSAFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            complement(4451..6019)
                     /locus_tag="ISB30_003562"
     CDS             complement(4451..6019)
                     /locus_tag="ISB30_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP1991155.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NASSPTGAPKLKSVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPGALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSTKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
     gene            complement(6394..8541)
                     /locus_tag="ISB30_003563"
     CDS             complement(6394..8541)
                     /locus_tag="ISB30_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8122..8124),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HAP1991156.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAESAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(8739..10205)
                     /gene="mdtP"
                     /locus_tag="ISB30_003564"
     CDS             complement(8739..10205)
                     /gene="mdtP"
                     /locus_tag="ISB30_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAP1991157.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLNGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAISAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(10202..12253)
                     /gene="mdtO"
                     /locus_tag="ISB30_003565"
     CDS             complement(10202..12253)
                     /gene="mdtO"
                     /locus_tag="ISB30_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HAP1991158.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSIVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPAKEQAQGAT
                     Q"
     gene            complement(12253..13284)
                     /gene="mdtN"
                     /locus_tag="ISB30_003566"
     CDS             complement(12253..13284)
                     /gene="mdtN"
                     /locus_tag="ISB30_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAP1991159.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVSDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(13303..13578)
                     /locus_tag="ISB30_003567"
     CDS             complement(13303..13578)
                     /locus_tag="ISB30_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991160.1"
                     /translation="MPTVLSRIAMQLKKIAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         3257 a         3622 c         3576 g         3214 t
ORIGIN      
        1 catcaatgtc tgatgcgatg ctgacgtgtc ttatcaggcc tacgtcagac agcgcaatag
       61 cctgatttgg cgtgattttt gtaggtcgga taaggcgttt acgccgcatc cgacatcaat
      121 gcctgatgcg acgtttgctg cgcctacctt aaccgttagt taatttttca aactcttcac
      181 aaccggtatc aaatccttcc atacagctca gattcaacca gtgcagcgcc ttttgtttat
      241 tcttctcgat aaaaccctct tcaccgttta agaacatcat tcccgcccag tattcggaat
      301 aaccggttcg ggaaatcgca gagctacgtt tgaaatacca ggttgctttg tcatcgtcgg
      361 ccttaatgcc cacgccgttg gcgtaaatca aaccaagcag catttgggca tcgacggcag
      421 agtcgttctc cagatcttcc gaggcgtttt ctaacagcga aatcgccttt ggataatccg
      481 gtttacccgc ttgcgtattt accagaatat gcgccagcgt tacttcacct tctttactgc
      541 ccgcttgcgc tgctttctcg gcaagcactt ttgcttgtgg atagtccaga ctgaccggat
      601 tggtgatttt gatctgcgcc aacagcgcgc aggcatcggc atcaccgctg tcggcggctt
      661 tctgtgccca atattcggct ttgctcaaat cgccggagct aaaccagctg tcggcaagaa
      721 aatattgtgc gcgtcggtct ccggcctctg ctgcctttaa atactggctg ccaggctcgg
      781 agtcgttcgc gtgggcaaag aatgtcaaaa acaacatcaa agtgataatt tttttcattt
      841 tgataatgaa gtttggtagg ggagagacag tataaagtgt tcgggtggac aaaaaaacaa
      901 ctgtaaaaag acctgaaagc agaatgaatt aatgcaatgt caggtttttt aatccatccg
      961 atgttccccc ttttccatgg cgtcgcgatc aaaaaatcgc tgaatttttc cctcggacat
     1021 caatgccgct cgatcagaca tatgcgcgat gacatcggca tcgtgactga ccagcagata
     1081 tgtcatttgg tgttccagct taagttgatt caacaaatta aggatttccg cttgtaccga
     1141 catatccaac gccgaggtgg gttcatccag cagaagaagt tgtggtcgta acaacagggc
     1201 gcgagcaata accacacgtt gccgttgacc accggaaagc tggtgtgggt agcgacgagc
     1261 ggcgtcgaaa ggcaagccaa cctgttccag tgcagtctgg acgcgttttt ctatttctct
     1321 ttcgccacga attttgagtg gttcagccag cgttcgccac agagtatgat tgggatgcaa
     1381 cgaggcatag ggatcctgaa acaccatttg cacattgcgg cggagatcgc cctgaaaacg
     1441 cattcctggt ttgatggtgt gcccaaacag tgacacctga ccgtgccagt cgcgctgtaa
     1501 tccagcaatt acgcgcaaaa tcgtggattt tccgcagcct gattcgccaa ttaaactaaa
     1561 tgtctctccg gcattaaccg aaaaactggc ggcagaaact gcggttttct cagcaaaagt
     1621 cacctgtaag tgattaattt caacaatcgt catggtattt ctccggcgtc attgccgtcc
     1681 tgtccagcgt cggcagcatc tgcccgtaag tttgtgcatt tgggcggcaa gtccacagtg
     1741 tgcgcgtgta aggatgcgtt gctgtaggta gcgcagcagc gtgcatttca tccaccttat
     1801 ttccctgata catcaccagc acccgatggc aaaattgggc aactaatggc aggtcgtgac
     1861 tgatcagcaa cattgccatc tgacgttgtt cacactgggt aaccagcagt tccagaatct
     1921 gattacgcag gcgcacatct agtgctgatg ttggctcatc ggcaatcaat acccggggat
     1981 cgttgatgag cgcaattgca atcatcactc gctgccccat gccgccagaa agttcacgag
     2041 gatagctttg cagtactcgc gtattgaggc cgacagcctg aattgcggtg tttattttct
     2101 cttctttctc gcggcggttc agacgttgat ggagcgtaag cgcctcttcc agttgcgctt
     2161 ttactgtctt tactggatta agcgcgtaac gcggatcttg caataccatc gcgatatcgt
     2221 taccgcgcaa ctgttgccat ccacgcgcgt taagggtgag tgcatcacgc cccagaatgt
     2281 taagtgtgtc cgcggttact acacccggtt tacgcaccag ccccatcagg gcgcgggcgg
     2341 tcatggactt acctgaaccc gactcaccga ccagcgccag acgttcattg cctaatgtaa
     2401 aactgacatt gttgacgacc cgggactccg gataatcgac gcagagattg ttgatactaa
     2461 gacgtgagtc agtcatgttg aggctccaga acatcgcgca agccatcacc cagcagatta
     2521 aaggccagac tggcaattaa gataattgcg ccgggagcgg cggcaatcca ccattgatca
     2581 aaaatgacct gcataccgtc ggcgatcatg gctccccatt ccgccattgg cgggcgagca
     2641 ccaagcccaa gaaaacccag accagcagcc gccagaatga tacccgctaa atccagcgcc
     2701 agacggacaa tggcagatgg caggcacaat ggcaaaatat gaccagccag cagacgcctg
     2761 ccgcggatgc ccatcatttc tgcggcggca agataatcac tgtgtcgcag acgttggatc
     2821 tcactccgtg cctgacgcgc ataagcaggc caggtggtta atgcaagggc cagtgcgccg
     2881 ttaaccagcc ccggccccag catggcaaca aaagcaaaag ccagaattaa acgcggcatt
     2941 gacattacta tgtcagtaaa acgcatcagc acgcgttcca gccagccgcc gtagtacccg
     3001 gctaaaatgc caatcagcag accgaccggg agtgtaatga ttgtcaccaa tgccaccagc
     3061 cccagtgccg ggcggctgcc gtagattaaa cgtgaaagta ggtctcgccc gtaactgtca
     3121 gtgccgagcc agtgctgacg actcggtgct tgtaatcgcg ccgccgcatc ctgccagtta
     3181 ggatcgtgag gtgccagcca cggcgcgaag agcgcaatta gcatcaataa caggataatt
     3241 atcaacccgc aaaacgcggc gggtgaatgg cgtaaacggc gtaaaaagag aaacatcggc
     3301 atcagcgtaa cctcggatcg gtcagccgga ccagcaggtc ggttaagtta tttatcaaca
     3361 caaagcagac gccaatcagc agcgttcctc ccatgattgc tgtggtatca cccgcgaaga
     3421 gcgctgtagt gaggtagcgc ccaatacctg gccatgaaaa gacggtttct gtcagtaccg
     3481 cgccttccag cattgatgta taggcgaggg cgataacggt gagcaaggtg ccgcgaatgt
     3541 tgggtaaaac atgacgtagc aaaattgtca tctcgctggc ccctttggcg cgcgccagca
     3601 gaatatattc tttattcatt tcgccaagac aggcggaacg agtcaggcgc gtaatactgg
     3661 cgagcgagaa gtacgccagt aataaaaccg gaagtatcaa atggttgatt gcattggtaa
     3721 atgcctcgcg atcgccagaa agccacgtat caatcagcgc aaatccgctg cggggttcta
     3781 tggtgaattg ataaatatca tccagcctgc ccggcccggc actccattgc agtctggcgt
     3841 agaacagcgc cagcatcagc agaccaagcc aaaaaacagg gacggaattt cccagtagag
     3901 ttaatgttcg cacggtgaaa tccagcggag aaccgacata acgggcgcaa agtacacccg
     3961 caatgacgcc gacaacagca ccgacaataa gcgccaacgt tgccagttca atggtcgccg
     4021 ggaatgctga tagcaagtcc tggagtactg gttgcccggt ggagctggcg atgcccagat
     4081 cgccgtgtgc cagattcatc aggtaatgcc agaactggac ggctaacggc tgatccaatc
     4141 ctaactggtg gcgtacctga tcataggtgg actggctggc gtgatcgcca acgatttgta
     4201 gtacgcgatc gaccggtgag agagccgaaa gcgcaaaggt aatcagcagc aggccgagta
     4261 gcgtcagagc aacggtaaac agcccttgca acagcgcgag cagagggcgt ttgcctcgcc
     4321 atcttgcgcg agtggaaaaa acgacagaca tgatctttcc atacagtgat aaatcagcgg
     4381 ctggcctgca atacaggcca gccctggtac cgcgcccggt aacatggctg gacgcgagtt
     4441 gcatacaggg ttatttggtt acctgatcaa accagaccat atcggcattc aacccttgtt
     4501 gatacccctg cacattatca cgcatgacaa tttgcgtttt gccttgatca ataaagacat
     4561 acggggaact acgttgtagt tcttgttgca ttttcttata caaatcaaga cgtttagtgc
     4621 tatcaggttc agcgatggcc gccagcgttt ctttgttcag ttccgggatt ttccagccgt
     4681 ttaacccggc aacggtgctg gatttgccat cgttataggc gaaagagctg gcgttggaat
     4741 gtgcgtcaaa ataatcaggc aaccacatac gaatggcccc ctgatgctgg tgggcgcgaa
     4801 cgcgagagta gacctgactc cctgcggctg gcagcaaatc aacttttaca ccgccctgag
     4861 caaagctggc ctgaattgac tgcgcgatgg taataaatgg cggtttattt tcgacatcaa
     4921 gcgtgaagtg cgggtctttg attccggctt tagcgaggat ctcttttgct ttggctggat
     4981 taaaggtaaa cggtgtttct tccagtgctc caggaaagcc cacgggcagg aaactctgat
     5041 gggtaaaata ttgtcctttt aagagatttt tggtgatgcc gtcgtaatcg accaaccagc
     5101 gagcggcctc ccagaacgca ggattactca tcagcgggtt atccttgtta ccggtattga
     5161 aggccaggta gttctgttcg gcggaggcaa tgctcatcac ttttacgcca ggtttaccct
     5221 gtaatgcatc gatttgatcg gctcccagat cacgagccat atcagcatca ccttgttgga
     5281 tcagcaggcg acgtgttgcc ggatcgggaa catttttgat gatgacgctt ttcagtttcg
     5341 gggcgccggt tggtgagctg gcgttggctt ccatcacgat ggcctgatgc ggttggtaag
     5401 tacgcatttt aaacgcgccg ctgccagcgg aatgcatctt cagccattca ttaccaaagt
     5461 cgttattttt ggcgtttggc gcaaccagct tctcgtcaac aattgaggcg attggtgtgg
     5521 agagaatatt cagtgccaga gccgggctga cgtcggcact ccattgcacc tggacggtat
     5581 ggtcatcgat tttcttcaac tggctggcga tattttccgg ctgccatccc agcacattga
     5641 gaataaacgc accggattta ttcagtgtga cagcccggac ataagagtaa atcacatctt
     5701 ccgggcgtac cggattacca gaggcaaatt tcgcatcgct tttcagtttt atggttagcg
     5761 ttttggctgc ggggtcagcc tgccaacttt ccgccagaat cggagtaatt ttcgtcggat
     5821 cattacggtc cggttgcact acgcgttgat acagacttgg tacagtctga atacttgaga
     5881 gttcattagc ttctgccggg tccaggctca caatatcatc cagcccctgg gctacaacca
     5941 acgtattagg cggtgtagcg gcaacggttg tggcggaaat tgtgcttaaa accagtaaag
     6001 gtaatagttt tttaatcata tgcttcccaa tccctgaaat tactttgagg ttattaatat
     6061 tctatcagac ggggttttgt tgcattaatc gcgccaaaat atcccgttaa tagaacgagt
     6121 taaacggtcg atgatttctc accttaccga aatttggttc atcaaatgta aagtattatt
     6181 acgttaggcc gcgatgagaa ttgagtaaag ataattctca tttgcaatcg tgactggaat
     6241 agaagaacaa ccgagaagac gtcgctaata accaggatag gctggtttga ctgcgcaggt
     6301 atgtataaaa aaatccggaa acagagagtc gtttccggat tgatacaaag gcgttgggta
     6361 atatcaccgc ttttaacgtg agtttaagcg gtattacgcc agtgccgctt cgcgcaggcg
     6421 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
     6481 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
     6541 ttcggtcacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
     6601 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
     6661 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
     6721 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
     6781 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtattcgt cggtgagttt
     6841 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
     6901 cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc ggcaaggcca
     6961 ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat ccgggcacag
     7021 atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat aacccatacg
     7081 agtggcaatt tcactgatga tttgccagtc cgttttcaga tcccacttcg gctcaaccgc
     7141 cttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
     7201 cgacgttgac ggtaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
     7261 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagtt ccgcgtcagt
     7321 ttgcagcgga tcttcgccca taatgtacgc cgctctcact tcaccatgcg ccacgcggtg
     7381 cggcagctcg ctgatacgat aaccggtatg cgctggcagg ctctctacgc cccaggcttt
     7441 ggcaaatttc tcgcggttag ccggatcttt cacgtactga tagcccggat acgtatccgg
     7501 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
     7561 cgcatgtggc ttaccgaggt taccggtcag catcgcgagg ctggtcagag aacgcacggt
     7621 ttccacgccc tgatagaact gggttacccc catgccccac aggatggcgg cgctttctgc
     7681 ctgggcatac atccgcgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
     7741 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
     7801 ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggc ccatcgcatt
     7861 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
     7921 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
     7981 gtgattcgcc acgattgggt gggaatccgc cgggttatac ccgaaaacga acactaaatc
     8041 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
     8101 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
     8161 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cgcgcgagga
     8221 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggc
     8281 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
     8341 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga agtcccagcc
     8401 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
     8461 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
     8521 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
     8581 tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc tgatattacg
     8641 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
     8701 acgaaatacc gcgtgacagc catcacgcgg tagacatttt attttttctc gacgacggga
     8761 gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
     8821 ctgtccagca tcagtaatga catctcttcg gcaagcactg gcaaccgggc ttcggtggcc
     8881 tgtaagcggc tggttaagcc gcgttggtag gcggcctcgg ctgcgcgctg ggtgaagcgc
     8941 gttgcttcta cgcgttcagc ctgcatctct cgctcgtcgt tgagcgtttg cagacgtgtg
     9001 ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg ttcaatcatc
     9061 atgttgctgg cggcgcgagt gccttcgaga ttggcattca accgtccacc gtcaaacagc
     9121 ggcaatttca gacctgggat aaagttgaac tggcgactgg tttttttgaa taaggtatcc
     9181 aggtggatag aatccaggcc gaaaaacgct ttgatatcaa agctcggata gaacagcgcc
     9241 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc ttgcagatcc
     9301 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa taccggtctg gacttgcggt
     9361 aatgccaccg gtttgatctc cggcatatcg ctggctcccg cgccaatcaa tgcacgcaga
     9421 gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc gaccgccaga
     9481 atctgtgccc gcgcgccgtg gaaaggcact tgcgcttcca gaccgtgcgc cactttactc
     9541 tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag atcgagcatc
     9601 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
     9661 actgctgcgg tttctgccag cgcggcatta tgcgcgctaa tggcggcagc aaccgctgag
     9721 cgatgcacac cccacaaatc gagatccagt ccggcaaaca aaccgactgt ggcttccgta
     9781 tagtacggcc cgtccatacc cagtgctggc gcatccatcg cataagggct taaaaagccg
     9841 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
     9901 gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc ttcggcgagg
     9961 gtgtgtgaac catttagagt ccgttggatc agcgcatcca gctgcgggtc attgagttgt
    10021 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    10081 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tacgtaccag ggcacagccg
    10141 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag ttgacgattg
    10201 atcattgtgt ggctccttgt gcctgttcct tggcgggcgt taatgccggg gctgtccagt
    10261 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtgggcagga
    10321 tggcctgcga ggcgggtgtt tcttcaagcg ttatttgagg agaacggctg agtgcggttg
    10381 ccagaccagc ggcgtatttc tccagtgcgt cggcaaactg acctgctctg tccggctgta
    10441 gtgcgttatc cagcgcctgc gccgagttcc aggtggcatc ccaggcgtga agaatatcgc
    10501 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct tcgctgtcca
    10561 gttgacgctc cagcaccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    10621 gacctatgcg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg cgtgggatcc
    10681 gcattacttt actcaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    10741 cgctttcagg ccagacaaag gtgtaaatca ccgccgacac cacggtacca atgatgatac
    10801 ccagagcgcg atcgcgaatt tccaccaggt cgtacaccgg gccaaaaacg ttttcgagcg
    10861 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    10921 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    10981 tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg ccgcaaaagg
    11041 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttgga ttagcgacga
    11101 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg tagaaggtgt
    11161 aacagatcaa acaggcgagc agcgttttta ccgcgtagcg catatagtct ggattggtaa
    11221 aagcgtcggc gactattgat ggcggtttgg tggcgggcgt tggcggcgta ttcgggtcca
    11281 tctgacccag ttgcaacaat gtctggcaga tattctccag gttacattcc cgtgccgcca
    11341 tcgcttcact ttcagtgatc cgccagtcgc tttgccagca ctgaccttcg gcaatggcat
    11401 gctgcagttt gttgatttct gaggctaatt tttgtcggaa ttcaataatt gcctgagaat
    11461 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    11521 cgcagctttg ccaccatgcg ctctgagttc gccagttggc atcgtccgcg aggcaaaaga
    11581 cattgagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    11641 ggctgtccgt caggtggcta atggcatcat caagccgatc attaagcgcc tgatgcatct
    11701 gcgtgatagc gcggctggga aaccacagca cgccgattaa cgtcatcaat aacgtcgggt
    11761 agaggccgac aacgatacac cacagggtta agcgcacgac cacttccgga tagtcgagca
    11821 tggcggggaa ggtttgcccg taaatagcga caatggcgac ggcgaaaaag accagcccca
    11881 gccgatgggt gcgcatcaaa aacatgcagc ccatcaggat cggcccggcg atgatcaatc
    11941 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    12001 ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc gactgaatac
    12061 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc atcgagatca
    12121 gaatcaccag taggcagcct acccagagtt gcacggtctg cggcacacga cctggccgcc
    12181 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac caccggtaat ggcagggagt
    12241 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    12301 ttccgggtca ggtttatcaa ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    12361 gttaatagaa cgcgacactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    12421 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cgctcatcag
    12481 gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcagtttcgc ggaaattggc
    12541 gatcacgtac cagtgacgag tgtcgattag ggtgaaaata gggcgcatgg cagaagcaaa
    12601 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    12661 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgggcgac
    12721 cagcgcatcg acgccgctga cggcactggc ggctgactgc gcctgcaaca atacggcatt
    12781 aaggtccgct tctgccgcac gctgggcggt tcttgcacgg tcaacctctt ccgcagaaac
    12841 aaaaccttct cgcagtagcg gttcggtacg gcgtaacgta tcgctggctt gtttcgcggc
    12901 ggcacgggct ttttctaccg tggcattaac cgagtcggca ccaaactgtt gcgcgtcaac
    12961 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cagctttcgc
    13021 cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct gtttcaccgc
    13081 ctggttgtcg ctgaccgcca gttctacaat gcgaccgctg acttccggca ccacatcaat
    13141 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    13201 gaaaacaagg gcaaccagcg ccaacgcaac cactaacaga gcagggaatt tactgcgagg
    13261 agcttttttc ggcgtacttt ccatgagcgc gtctcaaatt gattagaaaa atgccagcca
    13321 gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa atgccagatt
    13381 gatatttgtc cgctgaataa ttcgcctcgt gatgagcgtc aaaataaggc tggcaatggc
    13441 gcagaaaaac cagccgggat aataagcgcc gatcaccggg attgccggag ataatgagca
    13501 tcccgaaacc atgaagacgg gaattatcca ggctattttt ttgagttgca tcgctatgcg
    13561 agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa
    13621 tatcaatatc cattgaaggc gtcagtatgg ctattttctc gatgaataa
//
LOCUS       DACADF010000124        13606 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.126, whole genome shotgun sequence.
ACCESSION   DACADF010000124 DACADF010000000
VERSION     DACADF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.126"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(61..1077)
                     /locus_tag="ISB30_003568"
     CDS             complement(61..1077)
                     /locus_tag="ISB30_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1991161.1"
                     /translation="MAGKRKNPADNWMPPRVYQGKAAYEFRNKDNKAIRLCALDAPRS
                     AVWLAYEKAVGDEKERNTFQALTEQFMTSPDFMDLAVETRKDYTKYSGKVLPVFGKID
                     PDKIKPEHIRRYMDQRGLSSRTQANREKSFMSRVFRWGYERGYVQRNPCQGVKQFKEK
                     ARERYITDEEYQAVYEVSPDVVRVAMEIAYLCVARQSDVLSLQKDQLFDSGIYIRQGK
                     TGVKQIKAWSPRLQKAIALARSLPLKTGISSLFVIHQTTGGKYTRDGFNSRWRDVKAA
                     AQEKYPHLQIDFTFHDLKAKGISDLEGSLEEKQAISGHKNPRQTAAYDRKVKVVPVVG
                     GQRK"
     gene            complement(1046..1309)
                     /locus_tag="ISB30_003569"
     CDS             complement(1046..1309)
                     /locus_tag="ISB30_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HAP1991162.1"
                     /translation="MKSDHDIITREEMVELTGSPLKSKQCEALRRAGIFFMERADGHP
                     KTTWGHFMNPIKFRNLQEVTTRKDDEPDFGAIFNGRKEKEPSR"
     gene            complement(1519..1701)
                     /locus_tag="ISB30_003570"
     CDS             complement(1519..1701)
                     /locus_tag="ISB30_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991163.1"
                     /translation="MLKLTLKRGDAVHLVLADGTNGIIEARSRCELGLHLPENIKVTR
                     EKSAFTPQELITPNQK"
     gene            complement(1701..2270)
                     /locus_tag="ISB30_003571"
     CDS             complement(1701..2270)
                     /locus_tag="ISB30_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAP1991164.1"
                     /translation="MSTAIAIAKEYASLIDLLETADELTPEMIADTLEGMEGELGDKL
                     DAMMVICRNLQGNANTCAEEMSRLATRKTSFEGKEKAIRKHMLTCLQAAGLDKLKTAK
                     NTFTDAQGAIRVIIDSKDKIPDEYEGVSLVDVETVITPNKRAIKEVIESAEAVAAEIL
                     ARGETPPAELLNPVPGAHLERGERSLRIR"
     gene            complement(2267..4483)
                     /locus_tag="ISB30_003572"
     CDS             complement(2267..4483)
                     /locus_tag="ISB30_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991165.1"
                     /translation="MTIFNGLLEAKKGALKNGAIPALAIAIAAPNKKVAENIIIGKLW
                     EAYPDHGDNYFKPKIWEDAPGQPRPGVGEFDETFATEHSFDGEKWVVNTPVDSDCSSA
                     DISQVSDLMKLPARERFAAVLLFSHDANEVDSELLVQTREYLEMLDNSDTDSEDEVDA
                     FNRIVLDAMVACKPIEYMHIAGLNNLVHAILASCDTQEQNPTSWTISKFIKKWVENPG
                     KRDEMLPEVKPETASARPYKQTHATLDREIACALLPVAPEKITPSILKAADEIISQDR
                     EDFKRWSMALRTTDQILAYDRASVFGVIQSAPAKDTYHFPQSLRSHIDNWLQANGQRD
                     ANAVEEKPKEAAPKDDVKVTNHGGGRFSIDGMMSETPSNQGEKSEAANAGERSLQQLR
                     EQFVTPRHVYDVPENNAVSQREPAAITLAEETPPQEQLSEQVKDLVQNVDALVERIHA
                     EEHKRQNAISAIETELKDSDDTDNLALWKNVFKTDERFTSAFSQNGGGTSINGTYIAM
                     KATREFGPFGIGWGVEVLEERFDKGAPIVRKKQVGEKIEWDLIQDGVGGYLCEMHHTM
                     KVRVWYILNGVRGESEAYGCTPYIYDTKYGPTSDGEAPKKSWTDAVKKALSPLGFSAD
                     IFMGLYDNPEYRQRNKAEFDIKNASENAEDATRLRKELDEKLINVANTLAAAVTANEV
                     NKVFGLIAREVDVHRKAAETKGDKEYSSYLGSRLRRITDIKTERLAALTAAQEQTA"
     gene            complement(4514..4834)
                     /locus_tag="ISB30_003573"
     CDS             complement(4514..4834)
                     /locus_tag="ISB30_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991166.1"
                     /translation="MEARTIPVTLFIHYATSTFSHEKLLVATVDMSKNFPDRYILLES
                     REIEITVNQPEPIDIIGLQVEQLREQKQKTVADAQQRIAAIDDKIQQLLCIEYTPDTD
                     ELPY"
     gene            complement(5845..6258)
                     /locus_tag="ISB30_003574"
     CDS             complement(5845..6258)
                     /locus_tag="ISB30_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1991167.1"
                     /translation="MNTKTKNMAFASRLQRILKDLGWSQSELARHIGVTAQSVQAWCN
                     GVTPRKDKLDKLAKVTGYPVHFFFMNEGEYLDESTLHPGNYNQELTPQEQALLQLFRG
                     LPESEKNKLINELKEKREHFDLLLKELLEAKNQTK"
     gene            6357..6587
                     /locus_tag="ISB30_003575"
     CDS             6357..6587
                     /locus_tag="ISB30_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1991168.1"
                     /translation="MDKQLQDKILSVMSQSELGRRLGKKPQTVSLWFKGRVPGEEVLR
                     TSEALEWRVTPHDLRPDLYPNPDDSLPKTEAA"
     gene            6646..7122
                     /locus_tag="ISB30_003576"
     CDS             6646..7122
                     /locus_tag="ISB30_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991169.1"
                     /translation="MENQEELQKEILTWAARAGQELVTIEICRAWFSQGRNDELRLHE
                     FEDADGNVDWRAINNNRQKIFRWLRGETTAARRKTQVLASVMKAVLPAERRARLESPG
                     DPVLLATLAAKEGVEAINAVHLHFAPELTIQEIDEAIAALVATRGAVIRTAQDHHA"
     gene            7162..7386
                     /locus_tag="ISB30_003577"
     CDS             7162..7386
                     /locus_tag="ISB30_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991170.1"
                     /translation="MLRHIDRITWRNGWHLNGRPAHVAEIRPIFDGRVAAARSVWEKY
                     EEEKAKLREQNLSGAAYEAGCRVLSEALGI"
     gene            7383..8138
                     /locus_tag="ISB30_003578"
     CDS             7383..8138
                     /locus_tag="ISB30_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001023813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP1991171.1"
                     /translation="MNILPLLDRPIAFQRSFIRLEMGVTAALFLSQLTYWTNRTTDDG
                     WVYKTQDEWEEETGLSRYEQEGARKKLRGLGVLLERKQGLPARLYYKIDNDVLCQLLT
                     SAYKDAEKPHTGEGKTTRPVRGKPTNILTENTTEIITDGESADADTQQPADQRINYQV
                     ILDTYHEILPEMPPVKILTDGRKKHLRTFWKKFNFNESRWRAYLEFIGGNCRWMLEDR
                     PNGRGGFWRRKNLDYLITERCYVSVKEERANDK"
     gene            8128..9543
                     /locus_tag="ISB30_003579"
     CDS             8128..9543
                     /locus_tag="ISB30_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP1991172.1"
                     /translation="MINNAIALYSVDAEQAVLGCLMLNTDHDRTNAVFALLKPETFYI
                     NAHQVIYREIKGLFQADKPTDLITLTELMESKGLYERVGGFAYLAEMSKAAIPASMVN
                     YAKIVREKAILRYAVEKLNSCLEIMIQPADMTATDRITAVQQVIGQVAEHSRTGHKGG
                     LRPASEVVDDWIDDLERRFNDPAHAAGLTLGIESLDRLMAPKQALRGSLVVIGARPKM
                     GKTAAFNKIAVHFALNHRLPTLVFSLEMTDRSLIERMVAQEAKVNSEIFYLGPNDESE
                     LSLAIAKAGEIAESNMMIDSTAGVTLAHIVAECRKVKRMRGSVGLVAIDYLTLMKTES
                     AERRDIAYGDITTGLKNLAKELDCVVVLLTQLNRKLEERADKRPNPSDSKDTGQIEQD
                     CDVWIGLYRDAVYNENADPNLMEMLLRLNREGPSGTAYALMKNGSVIDISDEEVYRLT
                     NDRSEKNKRYSRSDNSRTNEF"
     gene            9582..9992
                     /locus_tag="ISB30_003580"
     CDS             9582..9992
                     /locus_tag="ISB30_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991173.1"
                     /translation="MTTTPGKLEYPSDKGHIDDGKNYLDVILWNMNVGPRARTRAVFV
                     PRPKAGNFSTPAKPARQASVAAAPAVKRKGKTHTGIAIRRNGERQVKLHETATTWCAS
                     PHETYDKITGQRIGAPGRCRLLLSSITPIAKKGA"
     gene            9994..10230
                     /locus_tag="ISB30_003581"
     CDS             9994..10230
                     /locus_tag="ISB30_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991174.1"
                     /translation="MAGQSDYLPPGLPHNRAKWPQEYQLKEHYDMRAAALIRQLFEKR
                     IPRGSVIEQIGMTPDTYREFFRERLNYWRGVMEQ"
     gene            10227..10538
                     /locus_tag="ISB30_003582"
     CDS             10227..10538
                     /locus_tag="ISB30_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP1991175.1"
                     /translation="MKYKRWVRAEVVIIKQCAGSMTVERIGQLIGRTGAAVRTKAREL
                     KICMYLRGNYHQSVKYLQEDIELARELHQSGINRQDIAEKLEMPIGAVNQFVYFERRI
                     S"
     gene            10535..10759
                     /locus_tag="ISB30_003583"
     CDS             10535..10759
                     /locus_tag="ISB30_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991176.1"
                     /translation="MKELFLAFVPRFINDQIALTDNGEQYEIACSMVDVNPGERYDAM
                     CDLKIFTWLGWAIPCGEPTNIRPFESREAV"
     gene            11441..12229
                     /locus_tag="ISB30_003584"
     CDS             11441..12229
                     /locus_tag="ISB30_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAP1991177.1"
                     /translation="MTVQISAESLSPLHHNQLPVITTELLASLYGTEVNGIQQNFKRN
                     IARFVVGKHFFKLEGNELRDFKNRLTDSQSVAKHTRSLILWTERGAARHAKMLETEQA
                     WEVFEKLEDCYFSQRPERRSTTTDDRTPLRDAVNILVGKKGMMYPDAYTMIHQRFAVS
                     HIEQLSQTQMLEAIEYIHCLLLDENHPTIPDFSFITTIKNGKVTGMRHVAKGEHLMTF
                     DAFKEIAERAGYLVIHSDNLRSMTLDKLMVMGKKSAISVANSAF"
     gene            complement(12404..13327)
                     /locus_tag="ISB30_003585"
     CDS             complement(12404..13327)
                     /locus_tag="ISB30_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HAP1991178.1"
                     /translation="MRLKYSVLLLAIALTGCDNKKDEIGCSSEITQSALMDLLKKSAY
                     EGLSEQVDKYPDVTNQTKRSTLDKIKLAISEISTTSSDTGSTMKTCEGTVTMTLSANE
                     YVQLSDAYRKNFNRNLDKQMESLFLENNANAFSKRITYTVQATDDQKNVFVKTSTDNP
                     ISVGAALLTSLSIITPIVEQQKIQQAKDAQQSQIEAEQQVQLRAQEQAKYEAEKQIER
                     QAQLQPQKADNLDQSRMAFANADSDLSTVWSKLTPAKKKDLLPSQRQWIKTKDAMCGK
                     VSMQGTDAEVKKMVDCQTQMTLSRIAFLRNQ"
BASE COUNT         3330 a         3180 c         3419 g         3677 t
ORIGIN      
        1 tcttctttct catttccgaa gatgcgtccg aagatgattt ttctctggac gcatcatcat
       61 tcattttctc tggccaccaa ctacgggcac tactttaact ttccggtcat atgctgctgt
      121 ctgtcgcggg ttcttatgcc cggaaattgc ttgcttctct tccaggctgc cttccagatc
      181 agagatacct tttgctttca gatcatgaaa tgtgaagtct atttgcagat gaggatattt
      241 ttcctgtgct gccgctttga catcacgcca acgagagtta aaaccatcac gggtatactt
      301 gccaccggta gtttgatgaa tcacaaacag gctactgatt cccgttttta atggcagaga
      361 acgagccaga gctatcgctt tctgcagacg aggcgaccag gctttgattt gcttaacgcc
      421 ggtttttccc tgacgaatgt agatcccgga atcgaacagc tggtctttct gcaatgaaag
      481 tacatcactc tgtctggcca cgcataagta ggcaatctcc attgctacgc gaacaacatc
      541 aggagacact tcataaacgg cctgatattc ttcatccgta atataacgtt cgcgagcttt
      601 ctctttaaac tgtttaactc cctgacaagg attcctctga acataacctc gttcataacc
      661 ccaacggaat acccgggaca taaagctttt ttccctgttt gcctgagttc ggcttgataa
      721 accgcgttga tccatatagc gccgaatatg ttcaggtttg attttatccg ggtcgatctt
      781 cccgaagaca ggcagaactt ttccggaata ttttgtgtag tctttccggg tttcgactgc
      841 caaatccata aaatcagggg aggtcatgaa ttgttccgtg agcgcctgaa aagtatttct
      901 ttctttttcg tcaccgaccg ctttttcata tgccagccat acggctgatc gtggtgcatc
      961 caatgcgcac aggcgtatcg ctttgttatc tttattcctg aattcgtagg ccgctttgcc
     1021 ctggtaaaca cgaggcggca tccagttatc tgctgggttc tttctctttc cggccattaa
     1081 atattgctcc aaaatcaggt tcatcatctt ttcgcgtcgt cacctcctgt aaattgcgaa
     1141 attttattgg gttcatgaaa tggccccagg ttgtttttgg gtgtccgtca gccctttcca
     1201 tgaagaaaat tccagcccgg cgaagagcct cacattgctt tgatttaagt gggcttcccg
     1261 tcagctcgac catttcttct ctggtgatta tgtcgtgatc gcttttcatg gtctttcctc
     1321 attatgtagc ttattgcgtc atctgccttc tggcaggcac gggcaatatc agactcggtc
     1381 aaagtctgtt tcctgacgct ggccgacagt cgaccaattt tgatatcaaa tgcggagagc
     1441 agagttgctc ccggttgcca tcgcagcatt gtgatcctcc ggtgtctggt gaatcgcaat
     1501 gctagcgatg gtgagttttt atttctgatt aggcgtaatc agttcttgtg gggtgaatgc
     1561 cgatttctcc cgagtgacct taatgttttc tggcaggtgt agcccaagtt cgcagcggct
     1621 ccgtgcttca ataatgccat tggtaccatc ggcaagtacc aggtgaaccg cgtcaccacg
     1681 ctttaatgtg agtttaagca ttagcgtatc ctcagtgacc gttcgccgcg ttccagatgt
     1741 gcgcctggta ccgggtttaa taactcggct ggtggtgttt ctccacgcgc cagtatttcg
     1801 gcggcaaccg cctccgcaga ttcaatcact tctttaatgg ctcgcttgtt tggtgtaata
     1861 accgtttcta catcaacaag tgacacgccc tcatactcat ccgggatctt atctttgcta
     1921 tcgataatta cccggatagc cccctgtgca tcagtaaatg tgttttttgc ggttttcagt
     1981 ttatccaggc cagcagcttg caggcaggtg agcatatgct ttcgaattgc tttttccttg
     2041 ccttcaaagg acgtcttacg ggttgccagg cgagacattt cttcagcgca ggtattggca
     2101 ttgccctgta aattacggca gatgaccatc atggcgtcca gtttgtctcc cagttctcct
     2161 tccatgcctt caagcgtgtc ggcgatcatc tctggcgtca gttcatcggc ggtttctaac
     2221 aggtcaatca gactggcata ttcttttgca atagcgatag cagtgctcat gctgtttgct
     2281 cctgtgcagc ggtaagggcg gcgaggcgtt cagttttaat gtcggtgata cggcgcagac
     2341 gtgatcccag atacgatgaa tattctttat cgcctttggt ttccgctgct ttcctgtgta
     2401 catccacttc gcgggcgatg aggccaaata ccttgtttac ttcgttggcg gttaccgcgg
     2461 cggccagcgt attggcaacg ttgataagct tctcatccag ttccttacgc agacgggtag
     2521 catcttccgc attctcgctg gcattcttga tatcaaactc agctttattc ctctggcggt
     2581 attccggatt gtcgtacagg cccatgaaaa tatcggcgct gaatccgaga ggggatagcg
     2641 ctttcttaac agcatccgtc caggatttct tcggtgcttc gccgtcgctt gttggtccgt
     2701 atttagtgtc gtagatataa ggtgtgcatc cgtaagcctc ggactcaccg cgtaccccat
     2761 tcaatatgta ccagactctc actttcatgg tgtggtgcat ttcacatagg tacccgccaa
     2821 caccgtcttg aatcagatcc cattctattt tttcaccaac ctgttttttg cgaactattg
     2881 gtgcgccttt atcaaagcgc tcctccagca cctctacccc ccagccaata ccaaatggcc
     2941 caaattcgcg agtcgctttc atcgctatat aagtgccgtt gatggatgtg ccgccgccat
     3001 tctgagagaa agcactggtg aagcgctcat cggttttgaa tacgttcttc cacaatgcca
     3061 ggttatccgt gtcatctgag tcctttaact ccgtttcgat agctgagatt gcattctgcc
     3121 gcttgtgctc ttcggcatgg atccgctcaa ccagcgcgtc aacgttctgc accagatcct
     3181 ttacctgctc gctaagttgt tcttgtggag gagtctcttc tgccagggta atagctgcag
     3241 gttccctctg tgatacggcg ttattttcag gtacgtcata cacatggcga ggcgtgacaa
     3301 actgctcacg caactgctgc aaactacgtt ctccagcatt tgcagcttcg cttttttcgc
     3361 cctgatttga gggtgtttct gacatcatcc catcaattga gaatcgtccg ccgccgtggt
     3421 tggtgacttt tacatcatcc ttgggtgctg cttcctttgg cttctcttca acagcatttg
     3481 catcacgctg cccgttagct tgcagccagt tgtcgatgtg actgcgcagg gattgtggga
     3541 agtggtacgt gtcttttgcg ggggcgctct gaataacacc gaatactgat gcgcggtcat
     3601 aggcaaggat ctgatccgtt gtgcgcaagg ccattgacca gcgtttaaaa tcttccctgt
     3661 cctgactgat tatttcgtct gccgctttca ggatgctggg ggtgattttt tccggggcaa
     3721 caggtaacag ggcgcaggca atttcacgat ccagagtggc gtgagtctgt ttgtaaggac
     3781 gtgcggatgc tgtttctggt tttacctccg gcagcatttc atctcgttta ccgggattct
     3841 ctacccattt ttttataaat ttggagatag tccaactggt cgggttttgt tcctgggtat
     3901 cgcaacttgc caaaattgca tgtaccagat tattcaatcc agcaatatgc atgtactcga
     3961 tgggcttaca cgccaccatg gcatcaagaa cgatgcggtt aaacgcgtca acctcatcct
     4021 cactgtcagt atcactgtta tcgagcatct ccaggtattc acgcgtctgc acaagcaatt
     4081 cactgtcgac ttcgttggcg tcgtggctga acagcagaac ggcggcgaat cgttcacgag
     4141 ccggcagttt catcaggtcg ctaacctgtg agatatctgc tgaactgcaa tctgaatcaa
     4201 caggagtgtt aactacccat ttttcgccat caaaactgtg ttctgtggca aacgtctcat
     4261 caaactcacc gacgcctggg cgcggttggc ccggggcatc ttcccagatt ttaggtttga
     4321 aatagttgtc gccgtggtca gggtaggctt cccacaattt gccgatgatg atgttctcgg
     4381 caactttttt gttaggggcg gcgatggcga tggccagcgc cgggatcgcg ccgtttttga
     4441 gcgcgccttt tttcgcttct aacaagccgt taaaaatggt cattggtctt tcctcattac
     4501 aggtcagtgg tttttagtag gggagctcat cggtatctgg cgtgtattcg atgcaaagta
     4561 actgctggat tttgtcatcg atagcagcaa tacgctgttg ggcgtcggcg actgttttct
     4621 gtttttgttc acgcagttgc tcgacctgta aaccgatgat gtcgattggc tcgggctggt
     4681 taacggtaat ttcaatttcg cggctttcca gaaggatgta cctgtctgga aaattttttg
     4741 acatatcaac agtcgcaaca agcagctttt cgtggctgaa agttgaagtt gcataatgaa
     4801 taaagagtgt tactgggatg gtgcgcgctt ccatagcgac tccttggtaa tgtatactca
     4861 gagccgatca gcgaatattg atcagcttcc atagcgtcag tgtcgttggt ctttcctcat
     4921 cacaagtttg gtcacttgtg ataaatccga atggtttggt cgccgttcgg ggtaactggc
     4981 ccgccttgtg cgggtctttt gctatctaaa gggtgccggt tacgtttccg gcgtcgttat
     5041 gtttccatta cgaccgtaca gggctggtca ggcccgggct ggtctttcct gttttgagct
     5101 ggtcaggctc atggtcaacg ctggtcaggc gtggtacttc ctccggtctt tccctggtgt
     5161 cacgctgcgg gtttggcctc tcctgttggg gactcaggca cagcgataaa acctggctgt
     5221 gtgaaaaaat ggcccatcgt gcggactggg caaagactaa acacagcaaa ttgatgatgg
     5281 gcgcccggaa tcgaaccggg taacgggcag ggagttcccg ttaaacacct gttcaccaca
     5341 accgggagcg cactccgcca tttcaaaatt taacgacaaa gctcaaagtt gagtcgatga
     5401 agtgcgctct cgtgttgtag ccaggactct tccctggtgg tcacaccgta tcgccttgat
     5461 ggtgaatcaa tcgctcatac ctggctgtgg cttgcacatt ccggctacct gctatggaga
     5521 caacgcgtta aggctccgct tccatccaga ccgcttcgac acatgtgcca tatgccggtg
     5581 aagtctttcc ctctgtcacc gtgttggcgc cgacgcgcaa aatttggtgc cgtctttccg
     5641 gctgtcagaa cgtgtttctg aacaactgcc gctgggtaag cgttgttgat gaaagtgact
     5701 ttacaatttg tatttgtaat ggtcaatgtg tttttaaaaa ataatttgta gtgaggggca
     5761 aaaaaatatt cactgtgtaa aggtgttttt gtgttagggg aaagttgcat gccagcagcg
     5821 gctggcctgg aaatttgaac tgaactattt tgtctggttt ttggcttcaa gtagttcttt
     5881 tagcagtaga tcgaagtgct ctcttttttc ttttagctca ttgattaatt tatttttttc
     5941 gctctcaggc agtcctctga atagttgcag tagagcctgt tcttgcgggg ttagttcttg
     6001 attgtaatta cccgggtgta gtgtggattc atccaaatat tccccttcat tcatgaagaa
     6061 aaaatgaaca gggtatccgg tcacttttgc tagcttgtct aatttatctt ttcttggagt
     6121 taccccatta caccatgcct gaactgattg agccgtaacc ccaatatggc gagctaactc
     6181 agattgagac cagcctaagt cttttagaat ccgttgtagt cggctagcaa aagccatgtt
     6241 tttcgttttt gtgttcatac cgtaaggata caaggtttaa ttgtagggag cattgcaaat
     6301 ataatttgta attacagtta ttgtttgtaa tgatgacgcg gacacaagga gtccatatgg
     6361 ataaacaact gcaagacaaa attctttccg taatgagtca atccgagtta gggcggcgat
     6421 tagggaaaaa accacaaacg gtaagcctct ggttcaaagg acgggttccc ggtgaagagg
     6481 ttttgcgcac atctgaagct cttgaatggc gcgtgacccc tcatgatctt agaccggatc
     6541 tttacccaaa cccagatgac agcttgccta aaacggaagc agcttaactg ttggtgagcc
     6601 ccaaatctga ttaagcgtaa tcaattttta gcgacaggag acgctatgga aaaccaagag
     6661 gaattacaaa aagagatttt gacctgggcg gcaagggcag ggcaggaact cgtcacgatt
     6721 gaaatctgcc gggcctggtt cagtcaggga cgcaatgatg agttgagact acatgaattt
     6781 gaggacgcgg acggcaacgt ggactggaga gccatcaaca acaatcggca gaaaatcttt
     6841 cgctggttac gtggcgagac aacggcggcg cgccgaaaaa ctcaggtgct ggccagtgtg
     6901 atgaaagccg tgctacccgc agaacggcgg gcacgtctgg agtcgccggg cgatcccgtt
     6961 ttgctggcaa cgctggcggc aaaagaaggg gtggaagcga ttaacgctgt acatctccac
     7021 ttcgcgccag agttaactat tcaggaaatt gacgaagcaa tagcggcgct ggtagcgacg
     7081 cgaggagcag tgatacgcac tgcgcaagac caccatgcat gagcgcctga ccagcgttta
     7141 acttataccg aggaaagacc gatgttaaga catattgacc gcattacctg gcgtaacggc
     7201 tggcacctga atggacgccc ggcacatgtt gcagagatcc gccccatatt cgatggccgc
     7261 gtcgcagctg cacgttctgt atgggaaaag tacgaagaag aaaaagcgaa actgcgtgag
     7321 caaaatctct ctggcgctgc ctatgaggct ggctgtcgcg ttctctcaga ggctttgggc
     7381 atatgaacat cctcccgtta cttgatagac ccatagcctt ccagagaagt tttatccgtc
     7441 ttgaaatggg cgtgactgcc gcattgtttc tgtcgcagtt gacgtactgg acaaacagaa
     7501 caactgacga cggctgggta tataaaaccc aggatgagtg ggaagaggaa acagggcttt
     7561 cacgatacga gcaggagggc gcgcgcaaaa aactacgtgg tctcggcgtc ttgctggaac
     7621 gtaaacaggg cttgcctgcc aggctgtact acaaaatcga taatgatgtg ttgtgccagc
     7681 tacttacgtc cgcatacaag gatgcggaaa aaccacatac aggtgagggg aaaaccacca
     7741 ggcctgtacg tggaaaacca acaaacattc ttacagagaa tactacagag attattactg
     7801 atggtgaatc ggctgacgcc gacacccaac aacctgccga ccagagaata aattatcagg
     7861 tgatactcga tacctatcac gagattttgc cagagatgcc accagtgaaa atcctcactg
     7921 acggcagaaa aaaacacctt cgcactttct ggaaaaaatt caatttcaat gagtcccgtt
     7981 ggcgggccta tctggaattt atcggtggca attgtcgctg gatgctggag gacaggccaa
     8041 atggcagggg aggattctgg cggcgtaaaa atcttgatta tctgattacc gaacgctgct
     8101 atgtttcggt caaggaagaa cgcgcaaatg ataaataacg cgattgcact gtacagcgtt
     8161 gacgcagaac aggctgttct gggctgtctg atgctgaata ccgaccacga cagaaccaat
     8221 gcggtatttg cgttactgaa accggaaacg ttttatatca atgcgcatca ggttatctac
     8281 cgggagatca aaggattatt tcaagctgat aaaccaactg acctgataac actgaccgaa
     8341 ctgatggaat cgaaggggct gtatgagcgc gtggggggat tcgcatacct ggctgaaatg
     8401 agtaaggcgg cgattccggc ctcaatggtc aactacgcga aaattgtacg tgaaaaagcc
     8461 attcttcgtt atgccgtgga aaagttgaat tcttgtctgg agattatgat ccagcctgca
     8521 gatatgactg ctactgacag gatcaccgcc gttcagcaag tgattgggca ggtggccgaa
     8581 cattcccgta ccgggcataa gggcggtttg cgtccggcaa gtgaagtcgt cgatgactgg
     8641 atagatgacc ttgagcgccg tttcaatgat ccagcacatg cagccggact aacgctcgga
     8701 atcgaaagcc ttgatcgctt aatggcacca aagcaggcgt taagaggttc attggtcgtt
     8761 attggtgccc ggccaaaaat gggcaagaca gccgcgttta ataaaattgc ggtgcatttt
     8821 gctctgaatc atcgtttgcc gacgctggta tttagcctgg aaatgactga cagaagtctc
     8881 attgaacgca tggttgcgca ggaagcaaag gtcaattcgg agatttttta tcttggccct
     8941 aacgatgaat ctgaattgag tctcgcaata gcaaaggcgg gggagattgc cgaatccaac
     9001 atgatgattg acagcacggc aggtgtaacc cttgcgcata ttgtcgctga atgtcgaaaa
     9061 gttaaacgga tgcgtggctc ggttggcctg gtggcaattg actatttgac gctgatgaag
     9121 accgaatcgg cagagcgtag ggatattgcg tatggcgata tcacaaccgg tttgaagaac
     9181 ctggcaaaag agctggattg tgttgtggtc ctgctgacgc aactcaaccg aaagcttgaa
     9241 gaacgtgccg ataagcggcc taacccgagc gacagtaaag ataccgggca gatcgaacaa
     9301 gattgcgatg tctggattgg cctctatcgt gatgctgttt ataacgaaaa tgccgaccca
     9361 aatctcatgg aaatgttgct acgtctgaac cgcgaaggac catcgggaac agcctatgcc
     9421 ctgatgaaaa atggctcagt tattgatatc agcgatgagg aggtttatcg ccttaccaac
     9481 gaccgctcag aaaaaaataa acgttattcc cgtagcgata attctcgcac aaatgaattt
     9541 taaccacgcc tgaccagcgt gaaataaccg aggaaagacc aatgaccacc actccaggca
     9601 aattagagta cccgtcggac aaaggacaca tagacgacgg taaaaactat ctcgacgtca
     9661 ttttgtggaa tatgaacgtc gggccacgtg cgcgcactcg cgcggttttt gtgccaagac
     9721 ctaaagcagg caatttttca acccctgcta agcctgcccg tcaggcatcc gtagccgcag
     9781 cgccagcagt gaaacgtaag ggcaaaacgc acaccgggat cgcgattcgc agaaatggtg
     9841 agcggcaagt gaaactccac gaaacagcga caacctggtg tgcctcaccg catgaaactt
     9901 acgacaagat aaccgggcag cgcattggcg cgcctggtcg ctgccgcctg ctgctgagtt
     9961 ccatcactcc gatagcaaag aaaggggcgt gatatggccg ggcaatcaga ttacctgccg
    10021 cccggcttac cacacaaccg cgccaaatgg ccccaggaat accagcttaa agagcactac
    10081 gacatgcgag cggcggcgct gatccgccaa ctctttgaga aacgtattcc gaggggaagc
    10141 gtgattgagc aaatcggaat gacgccagat acgtaccggg aatttttcag agagcgtctg
    10201 aactactgga gaggggtgat ggaacaatga aatacaaacg gtgggtgcgt gcggaagtcg
    10261 taatcatcaa acaatgtgcg ggcagcatga cagttgaacg tatagggcag cttattggca
    10321 gaaccggtgc agcggtacgc acaaaagcgc gcgagctgaa aatctgtatg tatctgcggg
    10381 gtaattatca ccagtcagtg aaatacctcc aggaagatat cgagctggca agggaattac
    10441 atcagtcggg tattaatcgc caggatatcg cagaaaaact cgaaatgccg atcggagcgg
    10501 tgaatcagtt tgtttatttt gagcggagaa tttcatgaaa gaactttttc ttgcatttgt
    10561 gccccggttt attaatgacc agatcgcact gactgataat ggtgaacaat atgaaattgc
    10621 ctgcagcatg gtggatgtga atcctggcga acggtatgac gcgatgtgtg acctgaaaat
    10681 atttacctgg ctgggttggg ctattccgtg tggagaacca accaatattc gtccgtttga
    10741 gagcagggag gctgtatgag tgaattgaaa atacgtcgca aggcaatagc acgcgttaaa
    10801 aatctattac cagtgcctgt caaatatcgt ttttgtggtg ggaatgtacg gattggtacg
    10861 gatgtatggt gattgcggag tgacgtaaaa cgaatcgttg tgggtgtaat atcgtcaata
    10921 tcattggttt cccggaggga aaggacatgt ttggcgataa actggagtta ccacggaacg
    10981 ggcggatagt gctctatatt tcagattaca aagtaacccg ggctgaggtg ttggaaccgg
    11041 aaaaacatct ggtcacgtta ccggacatta ttgagttgta taagcgggtc ggatatgtgg
    11101 acttgcatcg cgatgatgtt cccgttgagt gtatggaatg tgaacaccgg aggtttgaag
    11161 ttgattgaat tgacttagaa aaagtccggg gctatagttt ccgcgcagcc gcaaaatcgg
    11221 ttgccgggat tggcgtcccg gatatctaag tgacgcatag ccgcgttagc ggttttttta
    11281 tgtgttaggc acggccacat tcgcattatg gtgggctgtg tgggggcttc tgtggaagcg
    11341 ccgggttcac ttagccggtt acgccaaccc tgcacagctc accaccagat gattggcgtc
    11401 gctggtggtg atgaaaaaaa cactaagtga ggccactgcg atgacagtcc aaatatctgc
    11461 ggaatccctt tctccgctac atcataacca acttcccgtc atcactactg aattgcttgc
    11521 cagtttgtat ggcacggaag taaatggtat ccagcaaaac ttcaagcgca acatcgccag
    11581 atttgtcgta ggaaagcatt tttttaagct ggaaggtaat gaattacgcg acttcaaaaa
    11641 cagactgaca gatagtcagt cagttgctaa acatacccga tccctcattc tctggacaga
    11701 acgcggcgca gctcgccatg ccaaaatgct ggaaacagaa caggcctggg aagttttcga
    11761 aaagctggaa gattgttatt ttagccagcg tccagagagg cggagtacaa ctaccgatga
    11821 ccgtactccc ttgcgtgatg ccgttaatat actggtcggc aagaaaggca tgatgtatcc
    11881 cgatgcctac accatgattc atcaacgctt cgccgtctct cacatagaac aactgtcaca
    11941 gacgcaaatg ttagaggcga ttgaatacat ccactgcctg ctactcgatg aaaatcaccc
    12001 aaccattcca gatttttctt ttatcaccac tatcaaaaat ggaaaagtga ccgggatgcg
    12061 ccatgttgcc aaaggcgaac atctgatgac ctttgacgct ttcaaagaga tcgctgagcg
    12121 ggcagggtat ctggtgatcc acagcgacaa tctgcgaagt atgacactgg ataaattgat
    12181 ggtaatgggg aaaaagtcgg ctatatccgt tgctaattca gcattttgac aggttgtttg
    12241 gggcggaaat actctatttg cgcttaaaaa ggtatttgac cacgaccagc acgagaccga
    12301 atacctctgt caaagtaccg cccatataaa acagttacat atgcacttga tgactaagct
    12361 ggcaccaggt taacacctgg cgccagcttg aaggagactt cagttattgg tttctgagga
    12421 aagcgattct tgaaagggtc atttgcgtct ggcagtcgac cattttcttg acttcagcat
    12481 ctgttccctg cattgaaact ttaccgcaca tagcatcctt agttttgatc cactgacgct
    12541 gagaaggcag caaatctttt ttcttcgccg gggttaactt gctccagacg gtactcaaat
    12601 cagagtcggc attcgcaaat gccattcggg actgatcaag gttgtcagct ttttgcggct
    12661 gaagttgtgc ctgtctctct atttgcttct ccgcctcata cttagcctgt tcttgcgccc
    12721 tgagttgcac ctgttgttcc gcttcaattt gactctgttg ggcatcctta gcctgctgga
    12781 ttttctgctg ctcaacgatc ggggtgataa tggaaagcga tgtcagtaaa gcggcgccca
    12841 ctgatatcgg attatcagta gaggttttta caaaaacgtt tttctgatcg tcggtcgcct
    12901 gcacggtgta ggtgatacgt tttgaaaagg cgtttgcgtt gttctccaaa aacaggcttt
    12961 ccatttgctt atcgagatta cgattaaaat tctttctgta agcatctgaa agctggacgt
    13021 actcgttcgc agatagagtc atcgtcacgg tcccttcaca agttttcatt gtgctgcccg
    13081 tgtcacttga tgttgtggag atctcagaga tggctagttt gatcttgtcc agtgtgcttc
    13141 gtttggtctg attagtgaca tcagggtatt tatcgacctg ttcagagagt ccttcataag
    13201 cagatttttt taataaatcc atgagagctg actgggttat ttccgaagaa caaccgatct
    13261 catctttttt gttatcacag cctgtaaggg caatggcgag taagagtact gagtatttta
    13321 atctcatgaa ttccctttag agtaaggatt ggtttttaag acgcggtctg taatcagtcc
    13381 cgagagctac agtaaacaac gctgagcact gaacgcatca caggtaaggt aaaaggtgat
    13441 atcggcaaag gctgtgaaac ctttaattca acagatggga acggtatgcg caggatcctg
    13501 taacgacgct cccaaaaccg gaaacctgta aggcatgttt tcgctgctga acttaatata
    13561 cgaacctgca ctatcatgaa catattttat atcgttatac cccgag
//
LOCUS       DACADF010000125        13531 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.127, whole genome shotgun sequence.
ACCESSION   DACADF010000125 DACADF010000000
VERSION     DACADF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.127"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(323..748)
                     /gene="arsC"
                     /locus_tag="ISB30_003586"
     CDS             complement(323..748)
                     /gene="arsC"
                     /locus_tag="ISB30_003586"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAP1991179.1"
                     /translation="MSNITIYHNPACGTSRNALEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTHDQLIDFMLQHPILINRPIVVTPQG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGNRLK"
     gene            1083..1166
                     /locus_tag="ISB30_003587"
     CDS             1083..1166
                     /locus_tag="ISB30_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAP1991180.1"
                     /translation="MIDKAIIVLGALIAQLELIRFLLQLLN"
     gene            complement(1220..2572)
                     /gene="gorA"
                     /locus_tag="ISB30_003588"
     CDS             complement(1220..2572)
                     /gene="gorA"
                     /locus_tag="ISB30_003588"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP1991181.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCIGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2644..3486)
                     /locus_tag="ISB30_003589"
     CDS             complement(2644..3486)
                     /locus_tag="ISB30_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAP1991182.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDNRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3689..5731
                     /gene="prlC"
                     /locus_tag="ISB30_003590"
     CDS             3689..5731
                     /gene="prlC"
                     /locus_tag="ISB30_003590"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAP1991183.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            5739..6491
                     /gene="rsmJ"
                     /locus_tag="ISB30_003591"
     CDS             5739..6491
                     /gene="rsmJ"
                     /locus_tag="ISB30_003591"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAP1991184.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPAQLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6540..8009)
                     /gene="dtpB"
                     /locus_tag="ISB30_003592"
     CDS             complement(6540..8009)
                     /gene="dtpB"
                     /locus_tag="ISB30_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HAP1991185.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     QLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(8326..8760)
                     /gene="uspA"
                     /locus_tag="ISB30_003593"
     CDS             complement(8326..8760)
                     /gene="uspA"
                     /locus_tag="ISB30_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAP1991186.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9151..9486
                     /gene="uspB"
                     /locus_tag="ISB30_003594"
     CDS             9151..9486
                     /gene="uspB"
                     /locus_tag="ISB30_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAP1991187.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9557..11056)
                     /gene="pitA"
                     /locus_tag="ISB30_003595"
     CDS             complement(9557..11056)
                     /gene="pitA"
                     /locus_tag="ISB30_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAP1991188.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11288..12481
                     /locus_tag="ISB30_003596"
     CDS             11288..12481
                     /locus_tag="ISB30_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP1991189.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(12568..>13531)
                     /locus_tag="ISB30_003597"
     CDS             complement(12568..>13531)
                     /locus_tag="ISB30_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HAP1991190.1"
                     /translation="SGQSEYFVAGHVLISLSAICLALFTTAFIIISQLTHGMNKFYNK
                     LFPVIGYAGSATTMIWGWSLLASNNVMADEFVAGHVIFGVGMIAACVSTVAASSGHFL
                     LIPKNASGSKSDGTPLQAYSSTIGNCLIAVPVLLTLFGFIWSVILLRSADITPHYVAG
                     HVLMGLTAICACLIGLVATIVHQTRNTFSVKEHWLWCYWVILLGSLTIIFGIYVLISS
                     DASARLAPGIILICLGMICYSIFSKVWLLALVWRRTCSLANRIPMIPVFTCLFCLFLA
                     AFLAEIAQTDMAYFIPSRVLVGLGAVCFTLFSIVSILEAGSAKK"
BASE COUNT         3448 a         3425 c         3564 g         3094 t
ORIGIN      
        1 aatgggttaa cgtatgttga aattatttat tgtgcaaacc actgatagat cataatccag
       61 tacatcttta cagtgctgca ctgtatcgct gataactaag cagctcactg atacaccatg
      121 ctggacgcga taattggaat ctcacgcagg gagtactgtg taaatagcaa aatcgcctga
      181 tgcgctacga ctgtcaggcc tacaaataac atacaattca tcgaattatc ataacgttgt
      241 aggtcggata agacgcgtca gcgtcgcatc cgacataaat aaagcgcatt tttctaacaa
      301 cctgtggggg aatatagccg ctttatttca ggcgattacc cgcctcatca accactttct
      361 cgccatcttc cttggtgaac gcgccttttt gcgcatctgg cagaatttcc agcacgactt
      421 ctgaagggcg gcacaggcga gttccctgtg gcgtcaccac aatcgggcga ttaatcagaa
      481 tcgggtgctg aagcataaag tcgattaact gatcgtgagt aaatttatct tctgcaaggc
      541 ccagttcctc atacggctcg acgtttttac gcagcaacgc gcgtacggta atccccatat
      601 cggcaatgag ttttaccagc tcatcgcgcg tcggtggtgt ttccagataa tagataatgg
      661 tcggttctgt accgctgttg cggatcatct ccagcgcatt acgcgacgta ccgcaggccg
      721 ggttgtgata aatggtaatg ttgctcatat cagtatctca ttacaatgtg aaagagaggt
      781 gtagcgccag cgccaccagc gttacaaacc aaagcggaca agtcaaaaac acatatggtt
      841 taacgaatat gtaagtgtag agaaaggtgt aggtgtgaat taagcgttgg gcattcattt
      901 gaaaggaggt ctgaatcagg acgtaaaaaa accgacttcg cgtcggtttt tttactgttc
      961 agccctgagt tggtggctct ggctggagtg agatagccca catctaacaa tgagacatgc
     1021 tccgtgacag gcaatggatg agtaagcgga tgcattctca ctccatcgca tggagaaaac
     1081 gggtgattga taaagcaatc atcgttcttg gggcgttaat tgcgcagctg gaactgatcc
     1141 gctttctgct tcagcttctg aactgatagc ggaaacgtag ctaagggcta agagcacact
     1201 actcttagcc ctttaacatt taacgcattg tcacgaactc ttctgctgct gtcgggtgaa
     1261 tggcaaccgt gttgtcgaag tcttttttgg ttgcacccat cttcagcgcc accgcgaagc
     1321 cctgcaacat ttcgtccata ccaaaaccaa tgccgtgaat accgacaatc ttctcttccg
     1381 gtccaatgca taccagtttc atgcgacacg gctggcggtg agtggtgacg gcggtataca
     1441 tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc tcgcgcgcct
     1501 gcggctccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg gtcggaatgt
     1561 tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag agacggcgac
     1621 ctgctgcaac tgccaccgga gtcagttcca ctgcacccgt gttatcgccc accgcgtaaa
     1681 taccttcaac gttggtgttt tgatatttat cgacgacgat atagcctttt tcgttggttt
     1741 taacgccagc ggcttccagg ttgatgttgt cattggcagg ctcgcgacca atcgcccaaa
     1801 tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg ctgccgtcgg
     1861 catttttcac caccgctttc gggatggcgt tggtatgcaa ctgtgggcct tcggcgttca
     1921 tcacttcgac cagcgtttca gagatcatcg ggtcgaagct gcgcagcggc gcatgtttac
     1981 gcacaaacag atgcgtcttc gcaccgaggc cgttaatcac gcccgccagt tcaacggcga
     2041 tgtaacccgc gccaacaacc gctacgcgct ctggcaaagc cggaagggcg aagaagccat
     2101 cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga ccaccggtgg
     2161 cgatcagaat atgatcggcc gtgatggttt cgccgttgac ttcaatggtt ttggcatcaa
     2221 cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg ttctcatagg
     2281 aagtatgaat acggtcgata taggcggtac ggctggcgat caatgtttcc cagttgaatt
     2341 tattgatagt ggtatcaaag ccgtaatccg ggccgtacat atggatcgct tcacggattt
     2401 gtgccgcgtg ccacatcact tttttcggca cacagccaac atttacgcag gtgccgccca
     2461 gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg ttgatggagg
     2521 cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc atgacatatt
     2581 gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca gcaatggctg
     2641 caattactct ggcacgatcc agcttacggt ggcgtgcccg gtgcctgctg gaaccagttt
     2701 gctgtgcagc cacggcagca cgttattcat ctgctgttcc agcttccacg gcgggttaat
     2761 cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagca ccgccagttc
     2821 aatttgcaga attttgcgaa tgccggtcgc ttccaggtcg tggatcatgc gcttaatctg
     2881 ctggcgcagc accactggat accacaatgc gtaagtgcca gtggcgaaac gtttgtaacc
     2941 ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg gcggatcgat
     3001 aaggattaaa ccacggcggg aaaccggcgg tagtttggcc ttaagctgct ggaaaccgtc
     3061 ggctttttca acgcgcgcac gattgtcttt ctggaattcc gaacgtaaca acggataatc
     3121 gctcgggtgc agttcagtca gttgcaggct gtcctgttca cgcagtagct ggcgcgcaat
     3181 caacggcgaa ccggggtaat aacgcaactg cccgctacgg ttgaagtgtt ttaccacatt
     3241 gatgtacgcc tccagctccg caggcaaatc gtcctgctgc cagatacggg cgataccttc
     3301 gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc cggcccctgc
     3361 gtgggtgtcg agatagagaa acggtttatc tttctctttc agcgactcga tgatcaggct
     3421 ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt ggcgataact
     3481 gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg cgcacaaaag
     3541 tctatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg tttctcattg
     3601 aaattcacta cacttaaccc catgctacaa acattatgta aagcgcctgt tgagcgcttc
     3661 cttaacctct ttaaccagga ctgcgctaat gacgaatccg ttactgactc cctttgaatt
     3721 gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgacta aggcgctgaa
     3781 cgactgccgc gaaaatgtgg agcgcgtagt agcgcaaggg gcaccgtaca cctgggaaaa
     3841 tctctgccag ccgttggcgg aagtggacga tgtgctgggg cgtatcttct ccccggtcag
     3901 ccatctgaac tcggtgaaaa atagcccgga actgcgtgaa gcgtacgaac aaaccctgcc
     3961 gctgctgtca gaatacagca cctgggtagg acaacatgaa gggctgtata aagcatatcg
     4021 cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaaa aagcggttga
     4081 taacgcactg cgcgacttcg aactctctgg catcggtctg ccgaaagaga aacagcagcg
     4141 ttacggcgaa attgccactc gtctttctga actgggcaat cagtacagca acaacgtcct
     4201 cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggagctgg cggggatgcc
     4261 agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg aaggttattt
     4321 gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca accaggccct
     4381 gcgtgaagag atgtatcgtg cttatagcac tcgtgcttcc gatcaaggtc cgaacgccgg
     4441 taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg aactggcgca
     4501 actgctgggc tttgaaaact acgccttcaa atcacttgcc actaaaatgg cagaaaaccc
     4561 gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgcccgc aaggcgaaaa
     4621 agagctggca caactgcgcg ccttcgccaa agccgaattt ggcgtcgatg agttgcaacc
     4681 gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca tcagcgatga
     4741 acaactgcgt ccgtacttcc cggaaaacaa agcggttaac ggcttgtttg aagtggtgaa
     4801 acgtatttac ggcatcaccg ctaaagagcg taaagatgtt gatgtctggc atccggatgt
     4861 acgtttcttc gaactatatg acgagaacaa cgaactgcgc ggtagcttct acctcgacct
     4921 gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag gccagatgcg
     4981 taaagccgac ggttcgctgc aaaaaccggt cgcgtatctg acctgcaact tcaaccgccc
     5041 ggtaaatggt aaaccggcgc tgtttaccca tgacgaagtg atcaccctgt tccacgagtt
     5101 cggtcacggc ctgcaccata tgctgacccg cattgaaacc gctggtgttt ccgggatcag
     5161 tggggtgccg tgggatgcgg tcgaactgcc gagccagttt atggaaaact ggtgctggga
     5221 gccggaggcg ctggcgttta tctccggtca ctatgaaacc ggcgagccgc tgccgaaaga
     5281 gttgctggat aaaatgttgg cagcgaaaaa ttaccaggcg gcgctgttta ttctgcgcca
     5341 gctggagttc ggcctgttcg atttccgcct ccatgccgag ttccgtccgg atcagggggc
     5401 gaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtac cgtctccgtc
     5461 atggggccgt ttcccgcatg ctttcagcca catttttgcc ggtggttatg cggcaggtta
     5521 ctacagctac ctgtgggccg atgtactggc ggcagatgcc ttctcgcgtt ttgaggaaga
     5581 gggcattttc aaccgtgaaa ctgggcagtc gttcctcgac aacattctga gccgtggcgg
     5641 ttcagaagag ccgatggagc tgttcaaacg cttccgtggt cgtgaaccac agctggatgc
     5701 gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc ttaattgatg
     5761 aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg ctggaacacg
     5821 atgaagacaa cctgatggcg ctggtgttaa cgccggagca tctggaactg cgcaagcgtg
     5881 atgagccgaa acttggcggc atctttgttg attttgttgg cggagcgatg gcgcaccgac
     5941 gcaaattcgg cggtggtcgc ggagaggcgg tggcgaaagc ggtgggcatt aaaggcgatt
     6001 atttgccgga tgtggtggac gccaccgcag ggctggggcg cgatgccttt gtgctggctt
     6061 cggtcggctg ccgcgtgcgg atgctggagc gtaatccagt ggttgccgcg ttgctcgacg
     6121 atggtctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttacag gaacgtttgc
     6181 agttgattca cgcctccagt ctgacggcgc tgagcgatat caccccgcgt ccgcaggtag
     6241 tttatcttga cccaatgttc ccgcataagc agaaaagcgc gctggtgaaa aaagagatgc
     6301 gcgtgtttca gtcgctggtg gggccggatc ttgatgccga tggattgctg gagcctgcgc
     6361 agttgctggc aaccaaacgc gtggtggtaa agcgtccgga ctatgcgccg ccgctggcga
     6421 atgtcgccac gccaaacgcg gtagtcacta aagggcatcg ctttgatatt tatgccggaa
     6481 cgccggtgta aaagtaaagc ggggtagcga taacgctacc ccgctttctg caagaataat
     6541 taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa tagcatcact
     6601 actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata gacgggcaac
     6661 gtctcaagtg gatcggtaat gttgtctggt actgcggtaa atgttgccac atagccgccc
     6721 agcaagaacg cggcagcctg cgtcaggaac cacatcccga gaataaagcc catcaaatgc
     6781 tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacag ttcacctaag
     6841 ctctggaata agtacaccag cacgataaac catggcgatg tcagcccttg ggcatccgca
     6901 aaccacattc cagccgctgc agccgtcaaa aagcccagtg agcacataaa catgccgaga
     6961 gtaaacttca tcggcatcga gagatctttg cctttgttac ccagttgcgt gtaaatgcct
     7021 gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa gctgaccggg
     7081 ttgatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa gttcagcgac
     7141 gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag gacaaaggcg
     7201 acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa gaagatgatg
     7261 gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt gtgcatcagc
     7321 caggcgcaga cgaagatcat caccacgctg ccaagtaata cgtacaacag tttactgaag
     7381 ctcatcggcc ggaagtcggg ttcagaacca atatctttca ccattccacg acaggcgatg
     7441 taaaccagta atgcgataat taaccctgca ccgcacaggt tgtaggtgac tgaatagccg
     7501 aatttatcag cgatcacagg ggccagcgat aacgctatca acgagccgat attgatcgac
     7561 atatagaaca gggtgaatgc gccatcaagc cgtggatctt tcggcggata gcatttcgaa
     7621 agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt ccccagggcg
     7681 atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc aatcgccagc
     7741 acaagtgctc cgagaacaat ggtgcgtttg gttcccagca ggtggtcgcc aacatagcca
     7801 ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac aaaagcctgc
     7861 tcttgcgaga acccaagctg tttaacgaag aaaaccgcca gtacgccctg cacgccgtag
     7921 tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg aggttgctgc
     7981 agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc atcctgaaaa
     8041 gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga ttcttaatat
     8101 tgatgcggcg aatagattaa tacactatta caacagaaaa taaccagctg atcacactaa
     8161 tgatagaaaa tatgatggtg ttaaatgttg tattggcagg ggttttaatt gcgtacgcag
     8221 accgcaggcc agataaggcg tttacgctgc atcaggtaac aaggcgcgcc aaaaagcaaa
     8281 acgcccgcat atgcgggcgt tcaggaacac gtcgtggagg gaagattatt cttcttcgtc
     8341 gcgcagcgga acaatcagca tatcaacgtg aacggtgttg atcagctgac gtgcggaaga
     8401 catcagtttg ctccagaagt cctggtggtg accacaaacc accagatcca tatcgtattt
     8461 cttgattgca tcgaccagta cctggcccag atcgccgctg ccgctcaggg tttcagtgat
     8521 tgggtagcct gcattggttg aaagctcggt cagcgcatga tgtgtctctt cagagatgcg
     8581 tttctgcata tcacccagat tcacatcgat aagcccggtg tataggtcag agtagtttac
     8641 atctacgtgg atcagagaaa ctttcgcatt gtaggggcga gccatagaga ctgctttctc
     8701 taccagaact ttgctttccg gggagaggtc gaccgcgatg agaatgtgtt tataagccat
     8761 agtgttactc cttccataaa gttgtcgatg actggccagc tagcgtttct tgtgccgtta
     8821 cgtcacccgc gtcctgcgaa ctacgtgcgc ggggctaacc cagccgtaaa gattcagtgt
     8881 taagaccatc cttaccttat agcgacccgg atgatccgtc aatccgcctt gcttactcgt
     8941 tagataaaca aattttcgac atgaatatat tgatagtggt taaccttctg gaaaaaaaac
     9001 aacctgatct cctacactat ctatagagcc gctcggatgt tggtcaccac aaaagcggtt
     9061 tctccaggac ctggtgttaa ctgattggca gcgtatcggg agcggtagcc ccggggtgga
     9121 actccgcggg caggtcgccg gggaggaggt atgataagca ccgtcgcatt attttgggct
     9181 ttatgtgtcg tttgcattgt taacatggcg cgctatttct catcactacg cgcgttgtta
     9241 gtggtactgc gtaactgcga tccattgctc tatcaatatg ttgatggagg gggctttttt
     9301 acctcacatg gccaaccaaa caaacaggtg cgtctcgttt ggtatatcta tgcccaacgt
     9361 tatcgcgatc atcacgatga tgagtttatt cgccgctgtg agcgggtgcg tcggcagttt
     9421 attctgacca gcgcattgtg tggtctggtg gtggtcagcc tgattgcatt gatgatttgg
     9481 cattaagcac ttcagcaata atgacgcata aaaaaagcgg gccagtaaac tgacccgctc
     9541 gttttggtgc gtacaattac aggaactgca aggagagcca gtacagcccg ccggaaagca
     9601 gtaccgcagc cggaagggta aacacccagg ccatcagaat actggttacg gttttacgct
     9661 gtaagccgcc gccatctacc accatcgtcc ccgcgacaga agaggagagt acgtgagtag
     9721 tggaaaccgg catcccggta taacttgcca ggccgataga cacagccgcc gtcatctggg
     9781 cagacatccc ctgagcgtag gtcatgcctt tcttaccgat tttctcaccg atagtcgttg
     9841 ccacacgacg ccagccaatc atcgtaccga tacctaacgc cagcgcgacc gccatgatga
     9901 tccacaccgg cgcatactcg atggtgctaa gcatgtcgga cttcagtttc ttcaacaggc
     9961 gctgatcgtc agcactcacg ccaggcattt tcaccacttt atcgatagtg tcagaaacgc
    10021 acagcataat gcggcgcatc tggctacgtt gatcaagcga cagcttgtcg tagctttcca
    10081 catcggtagt caacatgcct ttcaggcggt tgagcgcgtt aatggtattc gacggatggc
    10141 aatggaactc cgcgggttgc gttgcgccag cttccggagc cggtactaac tgatcagcac
    10201 cggtcgcttg tttcagcagt gcaggatgct gctcaaagta agcttcgacg ttgttgatgg
    10261 catcacgggt acgggtgatt tcgtagccag tggcattcat gttcaccacg aagcctgctg
    10321 gtgcgacgcc aatcaatacc aacataacca gaccaatgcc tttctgacca tcgttcgcgc
    10381 cgtgcgaaaa cgccacgccg atagcggaaa ggatcagcgc aatacgcgtc cagaacggcg
    10441 gctttttctt gccgtctttc ttttcacgct ccgctggggt caggtggata cgggcgcgtt
    10501 tcttggtgcc gctccagtag cgacgcagca agaaaatcag accgccagca aacaccaggc
    10561 cgacaatagg ggaaacgatc agagaaccga aaatacttaa tactttcggg atattgagtg
    10621 catccaccac tgacgtcccg gtcatcaacg cattggttaa accaatcccg atgatcgcgc
    10681 caatcagcgt atgagagctg gatgcaggta aaccaaagta ccaggtaccc aggttccaga
    10741 taatcgccgc cagcaacata gagaacacca tggcaaggcc atgagacgat cccatattaa
    10801 gcagcagatc cgtcggcagc atatgcacaa tggcataggc aacactcaga ccacccagca
    10861 aaacacccaa aaagttgaat accgccgcca taaccacggc gagctgagaa cgcatcgcgc
    10921 gggtatagat aacggttgcc acggcgttgg ctgtgtcatg gaaaccattg atggcttcgt
    10981 agaacagcac aaaagccagt gcaagcaata ataacagccc ggtatgcaaa tccaggccag
    11041 caaacaaatg tagcatagga cgttacgcca ttttgaggac atgaacgcgg cgcattatca
    11101 gtgactttcg cggcgcgggc aaagtgaaat atagactttt tttgatttgc ctcctgtatg
    11161 gattccactc aaaaaataat tatcttatat aattcaggca aatacttcct tttagtaata
    11221 ttgatgctgg tgcgaccact gaggaatctt tacaattcac gcccgttttt tcttagagga
    11281 gcgcaacgtg gaaaggtttg atgccattat tataggcgct ggtgcggcag gtatgttctg
    11341 ttctgcgctg gcaggtcagg caggacgccg ggttctgctg atcgataatg gtaaaaaacc
    11401 agggcgcaaa atcctcatgt ctggcggtgg gcgctgcaac tttaccaacc tttatgtcga
    11461 accaggcgct tatctgagcc agaatccgca tttttgtaag tctgcactcg cgcgttttac
    11521 ccagtgggat ttcattgatc tggtcaataa acacggcatc gcctggcacg agaaaacgtt
    11581 agggcagctc ttctgcgatg actccgcgca gcagattgtc gacatgctgg tggatgagtg
    11641 cgagaagggc aatgtggcct tcagattgcg tagcgaagtg ctgagcgtgg cgaaggatga
    11701 tacaggcttc acgcttgaac tgaacggcat gactgtcggt tgcgaaaagc tggtcattgc
    11761 gaccggtgga ctgtcaatgc cggggctggg cgcatcgccg tttggttata agattgccga
    11821 acaattcggc ctcaacgtgc tgccgacccg tgcgggtctg gtgccattca ctctgcataa
    11881 accgttgctt gaagagttac aggtgctggc gggcgtggct gtgccttccg tgattaccgc
    11941 tgaaaacggt accgttttcc gtgagaactt actcttcacc caccgcggct tatctggacc
    12001 ggcggtgttg cagatttcca gctactggca accgggtgaa tttgtcagca tcaatctgct
    12061 accggatgtg gacctcgaaa ccttcctgaa tgagcagcgt aatgcacatc cgaatcaaag
    12121 cctgaaaaac acgctggcgg ttcatctgcc gaagcggttg gttgaacggt tacaacaact
    12181 cgggcaaatc ccggatgtct cgctaaaaca gctcaacgtg cgtgaccaac agacactgat
    12241 tagcacattg actgactggc gcgtacaacc caacggcact gaaggctatc gcactgccga
    12301 agtgacgctc ggcggcgtgg acaccaacga actctcttca cggacgatgg aagcgcgcaa
    12361 agtgcctggg ctgtacttca tcggcgaagt gatggacgtc accggctggc tggggggcta
    12421 taacttccag tgggcgtggt cgagtgcatg ggcttgtgcg caggatttgg tggcagaata
    12481 agaagcaaaa tgcgctctcg ataccagagg gattaacctg aataaaacca gataaggcgc
    12541 tcaacgcctt atctgaagcc ttttttatta ttttttcgct gaacccgctt ctaatataga
    12601 aacgatggaa aacagtgtaa aacaaactgc acctaagcca accagaactc gcgaaggaat
    12661 aaagtaagcc atatcggttt gcgcaatttc agccaggaac gcagcaagga acagacaaaa
    12721 cagacaggta aataccggaa tcatgggtat tctgttggct aacgaacagg tgcgtcgcca
    12781 taccagcgct aacaaccaca ctttggagaa aatactgtag cagatcatcc ccaggcaaat
    12841 gagaataatg ccgggggcca ggcgggcgct tgcatctgaa ctgattaata cgtagatacc
    12901 aaaaataatg gtaagtgagc caagcaaaat aacccaatag caccacagcc aatgttcttt
    12961 cactgagaac gtattccgcg tttgatggac aatagtggcg acaaggccaa taagacaagc
    13021 gcaaattgcc gttaacccca ttagtacgtg acctgcaaca taatgtggtg tgatatctgc
    13081 actacgtaac agtatgactg accaaatgaa accaaagaga gtaagcagaa cgggaacggc
    13141 aattaaacaa ttaccgattg ttgaagaata ggcctgcaat ggcgtaccgt cactcttact
    13201 gcccgaggcg tttttgggga tcaacagaaa atgtccggaa gatgccgcta cggttgatac
    13261 acaagcggca atcatgccaa cgccgaaaat aacatgaccg gcgacaaatt catctgccat
    13321 cacattattg cttgccaaca gtgaccatcc ccaaatcata gttgtagcag aaccggcata
    13381 accaataaca ggaaataact tgttgtagaa tttattcatg ccgtgagtta actgagatat
    13441 aataataaat gcggtagtga acaatgccag acatatcgca gaaagagaaa tcagaacatg
    13501 ccctgcaacg aaatattcac tttgcccaga a
//
LOCUS       DACADF010000126        13529 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.128, whole genome shotgun sequence.
ACCESSION   DACADF010000126 DACADF010000000
VERSION     DACADF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.128"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..669
                     /locus_tag="ISB30_003598"
     CDS             <1..669
                     /locus_tag="ISB30_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP1991191.1"
                     /translation="DGRDYDPDVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVK
                     EAVSHIHVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRG
                     TYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTF
                     SLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDA
                     GGGK"
     gene            666..992
                     /locus_tag="ISB30_003599"
     CDS             666..992
                     /locus_tag="ISB30_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP1991192.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            1002..1352
                     /locus_tag="ISB30_003600"
     CDS             1002..1352
                     /locus_tag="ISB30_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP1991193.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            1349..1795
                     /locus_tag="ISB30_003601"
     CDS             1349..1795
                     /locus_tag="ISB30_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP1991194.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRACANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            1792..2136
                     /locus_tag="ISB30_003602"
     CDS             1792..2136
                     /locus_tag="ISB30_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HAP1991195.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            2203..2919
                     /locus_tag="ISB30_003603"
     CDS             2203..2919
                     /locus_tag="ISB30_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1991196.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRMKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            2934..3308
                     /locus_tag="ISB30_003604"
     CDS             2934..3308
                     /locus_tag="ISB30_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1991197.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFAPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            3404..3613
                     /locus_tag="ISB30_003605"
     CDS             3404..3613
                     /locus_tag="ISB30_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HAP1991198.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            3661..6903
                     /locus_tag="ISB30_003606"
     CDS             3661..6903
                     /locus_tag="ISB30_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP1991199.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVARVQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGVLNGVAGNIDNVATAAGALVAVGVARYFGNMASGAVSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNELNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALAEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAES
                     DAQRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRNW
                     ASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGAEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            6896..7237
                     /locus_tag="ISB30_003607"
     CDS             6896..7237
                     /locus_tag="ISB30_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1991200.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNLTVRVVCREWSVTDNARHS
                     DFSCTIEQVVN"
     gene            7237..7935
                     /locus_tag="ISB30_003608"
     CDS             7237..7935
                     /locus_tag="ISB30_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAP1991201.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRQYQAYPIDGSGFEMSGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            7946..8689
                     /locus_tag="ISB30_003609"
     CDS             7946..8689
                     /locus_tag="ISB30_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1991202.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTSEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            8635..9267
                     /locus_tag="ISB30_003610"
     CDS             8635..9267
                     /locus_tag="ISB30_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAP1991203.1"
                     /translation="MCLHGDLQRFGRRLSLYVNTAAEAIRALSLQVPGFRRQMNEGWY
                     QIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTA
                     GASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTY
                     FSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGTVVVIGRQG"
     gene            9610..13083
                     /locus_tag="ISB30_003611"
     CDS             9610..13083
                     /locus_tag="ISB30_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAP1991204.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGKGTDARLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSEKRIADIRQVETAARYLGSALYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEI
                     TQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTP
                     AGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPA
                     FSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGITIS"
     gene            13151..>13529
                     /locus_tag="ISB30_003612"
     CDS             13151..>13529
                     /locus_tag="ISB30_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAP1991205.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVST"
BASE COUNT         3061 a         3306 c         4396 g         2766 t
ORIGIN      
        1 gacggtcgtg actatgatcc cgatgttctg cagaaggcgg tgaatgatgc ggtcgcaaat
       61 attccgcagc cggcagacgg taaaagtctc accccggatg atgtgcgtcc gatgcttgaa
      121 cagatggtga aggaggcggt aagccatatt catgttccgc gtgatggtcg tgactacgat
      181 ccggatgttc tgcagaaggc ggttctggat gcggtgagtg ccctgccggc tccgcaggac
      241 gggcgtgatg ccacggctct ggaaatactc cccgccattg acgatcaaaa atcctttccc
      301 cggggcacgt atgccacaca ccagggcgga ctctggcggg cgtatgaaaa aacgcacggg
      361 atgcggggat gggaatgcct ggttgacggg gtggcggata ttgacgtcag catgacgggt
      421 gagcggttgt tctctgtggt ggtccggcag agcagtggcc agcgtacgga aaaaacattt
      481 tccctgccgg tgatgctcta ccgcggtgtg ttcagagccg gtgaaaccta ccaccccggc
      541 gatacggtga cgtggggggg ctcgctgtgg cactgcaaca gtatgaccga agataaaccc
      601 ggagaagctc attcatcagc ctggaccctg gctgcaaaac gtgggcggga tgcaggaggc
      661 ggaaaatgac ggcattactg acactggaag agatcaaggc acatctgcgt gtcgaccatg
      721 acgcggatga tgacatgctg atggacaagg ttcgtcaggc taccgccgtg ctgctggcct
      781 acattcaggg cagccgggat aaagtgatcc gtgaggacgg tgaactgatc ccgggcgagg
      841 cattaacccg gatgaagggg gctgccatgc gactgaccgg gatgctgtac cggaatccgg
      901 atcttgcgga gcgggaagaa ctgcttcagg gggagctgcc gttttctgtt tccgtgctga
      961 tttacgattt gcgttgtccg acggtgttat gaggaggggg aatggcaata tctgcaggtc
     1021 gtctgacaca gatgataagt gttctgaacc cggtgttaac ccgtaacgct gccggagaaa
     1081 tgacggaaga atgggtgtca tgcgggaaaa ttcatgcgga tatccgaggc aggagcagcc
     1141 gggagcggat gcagtccggt gcggaaatgg cgcaggcgga aatccgcatc tgggtgcgcg
     1201 gtcagtccgg tcgggaaatc acggcagcgt cacgacttca tgtgctgagt ggtccatggc
     1261 gtgaccggat cctgaacgtt gtcgggctgc ccgtgccgga tgcgaccggc gggcgtctgg
     1321 aaattctctg tcggctggga ggggaaaaat gatcgaaacc ctgctggatt tttcggggct
     1381 ggaggacatc agccgcgatt tgcagcttct gagtggtgcg gaaaataacc gggtgctgcg
     1441 tgaggcaacc cgtgcgtgtg cgaatgtgct gaaagaagaa gtggtgtcac gggcaccggt
     1501 acgcagggga aaactgcgcc gcaatgtggt ggtcctttcc cggcgctccc gcgatggcgg
     1561 gatggaatcc ggtgtccata tccgtggtgt taatccggac accggtaaca gcgataacac
     1621 catgaaggcg gataacccgc gcaatgcttt ctactggcgg tttgtggaaa tggggaccgt
     1681 gaatatgcca ccgcacccgt ttgtgcgccc ggcgtttgat gtgcgcagtg aacaggcagc
     1741 tcaggtggcg attgcgcgga tgaaccgggc cattgatgag gtactgagac gatgacggag
     1801 gcggatttgt atcctcatct ggcgcatctt gccggcgggc aggtgtaccc gtatgtggtc
     1861 cccctgctgg atggcaggcc gtcggtggcg cttccgtggg tggttttcag cctgatttca
     1921 tcggtgtctg cggacgtgat gggcgggcag gcggagtcct cagtgtcggt gcagatagac
     1981 gtttatgccg ggactgtgac gcaggcgcgt cagatacgtc aggacgcccg tgaagccata
     2041 atgctgctgg ccccgggatc cgtcagtgaa atgcaggact atattccgga aaaccgctgt
     2101 taccgtgcaa ccctggagtt tcaggtcacg gtgtgacttt ttcttttttt ctacaaaacc
     2161 cataccccgc cgcgtgcggg ttttttatta tcaggaggca gaatgtctgc tttgtatgaa
     2221 cgctcacagc tgacgcaggt gatgatttca tctgccccgg cgactgctga aactatggat
     2281 aaggcggaat atctgcgcct ggactgcacc atcaaggaag tccagttcac cgctggtcag
     2341 aaacaggata ttgatgtgac cacgctctgc tccaccgagc aggagaacat caacggtctg
     2401 ggggcgtcgt ctgagatttc catgtcgggt aatttttatc tgaatcaggc ccagaacgcc
     2461 ctgcgtgatg cctatgacaa tgacgcgttg tatgcgttta aggtgctgtt tccgtccggt
     2521 aagggcttta agttcctggc ggaagtgcgt cagcacacct ggtcatccgg taccaacggc
     2581 gtggtggcag caacgttttc actgcgtatg aaaggcaaac cggtgtcctt tgtggtaccg
     2641 ctggcgtttg tgaaaaatct ggataaaaca cttaccgtga atacaggtgc gctgctgaca
     2701 atgtcagtca gtgccaacgg gggaacgccg ccgtataaat acgcctggaa gaaggatggt
     2761 cagccggttg acgggcagac gacagacacc ttcagtaagc caggtgcgca gtccgctgat
     2821 gcggggaaat atacctgcgt ggtgaccgat tcggcagaga aagcacagag tgtgacgtct
     2881 gttgaatgca ccgtgacagt gagcgcagcc gccggataag gggatgggtc atcatgaaaa
     2941 aggatctgaa aacgctggcg ctggccagac tgtcagggtt tcgtcataaa acggtgaagg
     3001 tgccggaatg gggtaatgtc agcgtggtgc tgcgggagcc ttcggcagag gcctggtatc
     3061 tgtggcagga agtgctcaat ggtgatggag aggatgacga taccctgtcg gtggtggcga
     3121 aaacccgccg taacctagaa gcggatgtga cgctgttctg cgatgtcctg tgtgatacgg
     3181 atctgcagcg ggtgttcgct ccggacgacc gtgagcaggt gctggccgtc tatggtccgg
     3241 tacatgcccg gttgctgcgt caggcactgg aactgatcgc tgatgcagag tcggccagaa
     3301 aaaagtagcc cgcccggaaa ttcgctttct gatgcgactt gcgctccgtc tggggcgcac
     3361 cttatccgaa ctgcggcaca gcctgagtgt gagcgaggcg atgatgtgga tggagttcga
     3421 cagggtatcc ccgctgggtg atgagcgcgg ggatatccgt aatgcacaga tcgtgaaagc
     3481 ggtttttggg gcacagggga tgaatgttgc actgaaggac gccatgctct gctggggcga
     3541 ggatgaggat aagccggagg tggatccgtt tgcggcgctg gaagacgcgc tgagcttcgc
     3601 agcacagtca tgaatgatga gaaccgctga ggcggttttt ttacgcccgg agaaaggtga
     3661 atggcgacgt tacgtgaact gattatcaaa atttcggcaa attcgcagtc attccagtcg
     3721 gagatccagc gggcttcccg catgggcagt gaatattacc ggaccctgca gaatggcggg
     3781 cgtcaggctg ccgcagtcgc ccgggtgcag cgacgcgccc tggctgagct gaacagccag
     3841 ttgacggaaa tccgcgcttc agctgccgga acggcggggg catttgcagg tgcctttgcc
     3901 accggacacc tgatttctct ggccgatgaa tggagttccg tgaatgcccg actgaaacag
     3961 gcgtcgcagt catccgatga attttcgtca tcacagaaag tgctgatgga tatcagccag
     4021 cggacgggca cggcattttc agataatgcg gccctgtttg cccgctcggc agcctcaatg
     4081 cgtgaatatg gttacagtgc tgatgatgtg ctgaaggtga cggaggccat ttccacgggg
     4141 ctgaaaatct ccggtgccag tacggcagag gcgggctcgg tgatcaccca gttcagccag
     4201 gcgctggcac agggtgtgtt acgcggtgag gaatttaatt cggtcaatga aagcggagac
     4261 cggatcgtac gtgcactggc tgcgggtatg ggcgtggccc gtaaagacct taaggcgatg
     4321 gcggacgacg gcaaactgac ggcggataaa gtcgttcctg cgttaatcag ccagctgggg
     4381 gtattgcgtg atgaatatgc cgccatgccg gaaacggtct ctgacgggat cacaaaggtg
     4441 gaaaacgcct ttatggcctg ggtgggtggc gcgaatgaag ccagcggagc gacgaaaacg
     4501 ctctccggcg tgctgaacgg tgttgccggt aatattgata atgtggcaac agccgcgggg
     4561 gcgctggttg ccgtcggggt tgcccggtac tttggcaata tggcctccgg agcggtgtct
     4621 gccacggcag gacttgtgac ggcagcacgt aatgaagtgg cactggcgga agcacagctc
     4681 agggggacgc agattgccac ggcgcgggca agggcagccg tgtaccgtgc acagcaggct
     4741 gtggcggcag cccgcgggac ggagatgcag attgctgcag aagcccgtct ggcggccaca
     4801 caggaacgcc tgaacagaaa tattgctgcc agaaccgcag cccagaatga gctgaacagt
     4861 acaacggcgg tgggctcacg tctgatgact ggtgcgttgg gactggttgg tggcgtaccc
     4921 ggactggtga tgctgggggc agcagcatgg tatacgctgt accagaatca ggagcaggcc
     4981 agggagtcag cgcgccagta tgcactgacg atagatgaaa tcgcgcataa aacgccgtca
     5041 atgtctttgc ctgaagcctc agataatgaa ggacgaacac gggcggcgct ggcagagcag
     5101 aaccggctga ttgatgaaca ggccagccgg gtgaaatccc tgcaggaaaa aatcgctgga
     5161 tatcagtatg ttctggctaa ccctggctgg acaaccggtg acggattcat gataaaccat
     5221 ctgacatcgg tgaagaccgt gacggaaggg ctttctcagg caacagagca gcttgccgtt
     5281 gagcagtccc gtctggcaca gatgcaggaa aaagcgcagt ccattcagga tgtgcttgcc
     5341 gggctggaag accgtcgtgt ggcgttaatt cgtcagcagg cggcagagca gaataaggtg
     5401 taccagtcca tgctggttat gaacggtcag catacggaat tcaaccgtct gctggggctg
     5461 gggaatgaac tgcttcagca gcgtcaggga ctggtgaatg taccgttacg gctgccgcag
     5521 gccaccctgg atgataaaca gcagagcgcc ctgacaaaaa cagaacgtga gctggccctg
     5581 tcccgactga agggggagga aaaagagcgt gcccggctgg ggtatgcggc ggatgacctt
     5641 ggttttgtgg gggattcgta tcaggaggcg agacagcgtt atatcagtaa tgctctggaa
     5701 gcctggcgta ataacgaggc gaacaaacct aaatcccggg gtggaaaatc agagacggaa
     5761 aaagcggaag acagtttttc ccggctactg aagcagcaga aagagcaact ggcactggcg
     5821 ggtcagaata cagagctggc gaagctgaag taccagactg cgcagggcga actgaaaacc
     5881 ctgacggaga tgcagaagca ggaactgctg cgtaacgcgg ccctgattga ccagcaaaaa
     5941 atccgggaac agttgcgatc ccgggaagag accctgaaga atgataatgt ggctgcgcgt
     6001 gcatcaaatg aagccgaact gctgggatac gggcagggag aacgagccag ggaacgcatg
     6061 cgggagttgc agcagatccg tgacagcttc cgccagaagg atgcggacct tcagtctcag
     6121 tatcagaccg gggatatcag tgaggatttt tacagacagg cacgggcaca gaacgcgcag
     6181 tatctgagcg aacgccttaa ggaccaggca gccttttatg ccgaatcgga tgcgcagcgt
     6241 gcggactggc agaaaggctt gcaggagggg ctcagtaact gggtggacaa tgcatccgat
     6301 tacgcctcac aggcagcaca gcttgcgacg gagggtatct cagggatggt gaataacatc
     6361 acggagatgc tgaacggaaa caaagtggaa tggcgcaact gggcctcatc cgtactgcag
     6421 gaaatctcaa aagttcttat gaatgctgcg attgtcaacg gaattaagac ggcggcaaac
     6481 ggtatgtccg gtgcgggagg atttattggc agcattggtg actggctggg cggtgcggtg
     6541 gccaatgcaa aaggcggcgt gtatacctcg gcaaacctga gtgcgtacag caacagcatt
     6601 gtggacacgc ccacgtactt tgcgtttgca aaaggggccg ggctgatggg ggaagccgga
     6661 cctgaagcca ttatgcccct gacccgggcg gcggatggct cgctgggcgt acgcgcggtg
     6721 ggcagtatga acggcagtgc cggtctggtg tattccccgg tctaccacat cgccattcag
     6781 aatgacgggg ctaacggaca gataggggcg gaggcggcag gcagtcttgt gcagctgatt
     6841 gaccagcggg tgcaggcggt gatgctgtcc atgcgacgtg acggaggaat gctgagtggc
     6901 tgagataaaa acgctgcatc tggtcccgcg tgaagggatg caggtgagtg agaaaccgtc
     6961 ggtggtgagg gttcggtttg gtgacggtta tgaacagcgc cgaccgacgg gacttaatgc
     7021 cagactgaag acgtttcagg cggtgttccg ggtgacggat gaaccaaccc ggcgctggct
     7081 ggatgaattt ttatcgtggc atggtggtta ccgtgccttt ttgtggcgac cgccgaaaca
     7141 taacctgacg gtgagggtgg tgtgccggga gtggagcgtc acagataacg ccaggcacag
     7201 tgatttcagt tgtacgattg agcaggtggt gaactgatgc aggatattcg cgaagaaagt
     7261 ctgaacgagt cggttaagtc agagcagtca ccgcgggtgg tactctggga aatcgacctg
     7321 acggtacagg gcggtgagcg gtattttttc tgtaatgagc tgaatgaaaa aggggagccg
     7381 gtcacctggc aggggcggca atatcaggca tacccgattg acggcagcgg ttttgagatg
     7441 agcgggaagg gcagcagtgc cagaccgtcg ctgacggtgt ccaatctgtt cggtctggtc
     7501 accgggatgg cggaagacct gcagagtctg gtgggggcca cggtggtccg ccgccgggtg
     7561 tatgcccgtt ttctggatgc ggtgaatttc gttgcgggca atccggaggc ggacccggag
     7621 caggagctga gtgaccgctg ggtggtggag cagatgtcgc agctgacagc catgacggcc
     7681 tcgtttgtgc tggctacacc gaccgagacg gatggggcgc tgtttcccgg tcgcatcatg
     7741 ctggcgaaca cctgtatgtg ggattaccgg ggagatgaat gcgggtataa cggtcctgcg
     7801 gtggcggatg agttcgacaa ccccaccacg gatatccgta aggacagatg cagcaagtgc
     7861 atgcgcgggt gtgaactgcg caggaatgtc ggcaattttg gcggtttcct ttccattaat
     7921 aaactttcgc agtaaatccc ggtttatgac acagactgaa tcagcgattc tggcgcatgc
     7981 ccggcggtgt gcgcctgcgg agtcgtgcgg cttcgtgata agcacgtcgg agggggagtg
     8041 gtatatccct tgtgtgaata tttctgcaga gccggaggcg tattttcgta tcgcaccgga
     8101 agactggctg cgggcagaga tgcaggggga gattgtggca ctggtccaca gtcatcccgg
     8161 tgggctgccc tggctgagcg aggctgaccg gcggctgcag ataaaaagcg cactgccctg
     8221 gtggctggtc tgccggggtg acattcacaa attccgctgt gtgccacatc tgacaggacg
     8281 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     8341 ggggactgaa atgccggatt ttcatcgcga ggatgactgg tggcgcaacg gtcagaacct
     8401 ttacctggac aatatggcgg tcaccggctt ttaccgggtg cccctgtcct ctgcacaggc
     8461 gggcgatatt ctgctgtgct gctttggtgc ttcggtaccg aaccatgccg ccatttactg
     8521 cggcaacggt gagctgcttc accatctgcc tgaacaactg agtaaacggg agaggtattc
     8581 cgaaaaatgg caacgacgaa cgcattctgt ctggcgtcac cgccactggc acgcatctgc
     8641 cttcacgggg atttacaacg atttggccgc cgcctcagcc tgtatgtgaa cacggcagcg
     8701 gaagccatcc gggcgctgtc gttacaggtg ccgggctttc gccgtcagat gaacgaaggc
     8761 tggtaccaga tacgtattgc cggttatgac acggcaccgg aggcggtgta cgcccgtctt
     8821 cacgaacagc tgggtgaggg aacggtcatc catattgtgc cgcgactggc cggggccgga
     8881 aagggtggac tgcagattgt gctgggggcg gcagccatcg tgggctcttt cttcactgcc
     8941 ggggcatcaa tggcgttatg gggttcagcc ctggcagccg gtggtttttc tgccaccacg
     9001 atgctgtttt cacttggagc cagcatgatt ctgggcggtg tggcccagat gctggccccg
     9061 aaggcaaaaa caccggatta ccgcgcaacg gataacggca gacagaacac gtacttttcc
     9121 tcgctggata acatgattgc ccaggggaac ccgatgccgg tgccttacgg ggaaatgctg
     9181 gttggctccc gccgtatatc ccaggacatc agcacccgtg atgaaggcgg gggcggaacg
     9241 gtcgtggtta tcgggcgaca gggataaaac ataaaaaaat cccgcagtga tcgcggagct
     9301 gcggggacag acaaatgaag atcaatgtta aggagttgtt tttgttactc gggcaaaaaa
     9361 acactaacgc agcgaaatta taagcgccac agtcagtgtg tgaaaatgtg aagatattca
     9421 gaatttttat gccattaccg gttttaacca acaggattat cggtgggcat gaaagaaaac
     9481 cccggtatct gctgataccg gggtttctct ttagcatggc agaaatgtgt ttcatgcttt
     9541 tcgggcgaag gatatccgac ttctgtacgg aatggcaagt ggcggttaat ttattcaggg
     9601 gaaggctgta tgggaaaagg tggcggtaag gcacacacgc ctcgtgaggc gaaggataat
     9661 ctcaaatcca cgcagatgat gagcgtgatt gatgcgattg gtgagggacc gatagaaggt
     9721 ccggtgaagg gactgcagag tattctggtg aacaaaaccc cactgacgga cacggacggc
     9781 aatcccgtga tacacggtgt gacggcggtc tggcgcgccg gggagcagga gcagacacca
     9841 ccggaaggct ttgagtcctc cggagctgaa accggactgg gcgtggaagt gacgaaggca
     9901 aaaccggtga cgcgcaccat tacgtccgcg aacattgacc gcctgcgggt taccttcggg
     9961 gtgcagtcac tggtggagac cacctcaaag ggtgaccgta acccggcatc cgtccgcctg
    10021 ctgattcagt tacagcgtaa cggtaactgg gtgacagaaa aggacgtcac cattaacggc
    10081 aagaccacct cacagttcct ggcctcggtg attctggata atctgcctcc ccggcccttt
    10141 aacatccgga tggtcaggga gacggcggac agcaccacgg accagctgca gaataagacg
    10201 ctgtggtcgt catacaccga aatcatcgat gtgaaacagt gctacccgaa cacggccatt
    10261 gtggggctgc aggtggatgc ggagcagttc ggcggccagc agatgacggt gaactaccat
    10321 atccgcggtc gcatcatcca ggtgccgtca aactatgacc cggaaaaacg cacgtatagt
    10381 ggtatctggg acggcagtct gaaaccggca tacagcaaca acccggcctg gtgtctgtgg
    10441 gacatgctga ctcacccgcg ctacggcatg ggaaaacgtc tgggggcggc ggatgtggac
    10501 aagtgggcgc tgtatgccat cgggcagtac tgcgaccaga cggtcccgga tggtttcggg
    10561 gggaccgagc cgcggatgac ctttaatgcg tacttggcac aacagcgtaa ggcgtgggac
    10621 gttctcagtg atttctgctc tgcgatgcgc tgtatgccgg tatggaacgg tcagacgctg
    10681 acgttcgttc aggaccgccc gtcggatgtg gtgtggccgt acaccaacag cgatgtggtg
    10741 gtggatgata acggcgtggg attccgctac agcttcagtg ccctgaagga ccggcacacg
    10801 gcggtggagg tgaattacac cgacccgcag aacggctggc agacctccac ggaactggtg
    10861 gaagacccgg aagccatact gcgctacgga cgcaacctgc tgaagatgga cgcgttcggc
    10921 tgtaccagcc gcggtcaggc ccaccgtgcc ggactgtggg tgataaagac cgaactgctg
    10981 gaaacgcaga cggtggattt cacgctcggg tctcaggggc tgcggcacac acccggtgac
    11041 attattgaaa tctgtgataa tgactatgcc gggaccctga ccggcggacg tgtcctgtcc
    11101 attgatgctg ccacccgcac cctgacgctg gaccgtgagg ttaccctgcc ggagacaggt
    11161 acatcggcgg tgaacctgat taacggcagc ggtaagccgg tgagtgtgga catcaccgca
    11221 caccccgcgc cggaccggat acaggtcagt accctgcctg atggtgtgga gacatacggg
    11281 gtgtggggac tctccctgcc gtcactgcgc cgtcgcctgt tccgctgtgt ctccatccgg
    11341 gaaaacacgg acggcacctt tgccatcacg gcggtgcagc acgtaccgga aaaagaagcc
    11401 atcgtggata acggtgcccg ctttgagccg cagtcaggtt ccctgaacag cgtcatccca
    11461 ccggcagtgc agcacctgac ggtggaggtg agcgcagctg acggccagta tctggcgcag
    11521 gcgaaatggg acacgccgcg ggtggtgaag ggtgtgcgct tcagtctgcg cctgaccagt
    11581 ggtaagggaa cggatgccag actggtgacc accgccatca ccgcagacac ggagcaccgt
    11641 ttcagcggcc tgccgctcgg ggaatacacc ctgacggtgc gggcgataaa cagctatggc
    11701 cagcagggtg aacctgccac caccaccttc cggattgccg caccggcagc accgtcgcgg
    11761 attgagctga cgccgggcta ttttcagata accgcaacgc cacatcttgc cgtttatgac
    11821 ccgacggtac agtttgagtt ctggttctcg gaaaagcgga ttgcggatat caggcaggtt
    11881 gaaaccgcag cccgctatct tggctcggcg ctgtactgga tagctgccag tatcaatatc
    11941 aaaccgggcc atgattatta tttttatatc cgcagtgtga atactgttgg caaatcggca
    12001 ttcgtggagg ctgtcggtcg ggcgagcgat gatgcggaag gttacctgga ttttttcaaa
    12061 ggagaaatcg ggaaaacaca tctggcccag gagctgtgga cgcagattga taacggtcag
    12121 cttgcgccgg acctggctga aatcaggacg tccattacga atgtcagcaa tgaaatcacg
    12181 cagaccgtca ataaaaaact ggaaaatcag agtgcggcaa tccagcagat acagaaagtt
    12241 caggttgata caaataataa cctgaacagc atgtgggccg tgaaactgca gcagatgcag
    12301 gacggacgcc tttatattgc gggtatcggt gccggtattg agaatacgcc agcaggaatg
    12361 cagagtcagg tgctgctggc ggcagacagg attgcgatga ttaatcctgc gaatggcaac
    12421 acaaagccga tgtttgttgg tcagggcgat cagatattta tgaatgaagt gttcctgaaa
    12481 tatctgacgg ctcccaccat taccagcggc ggtaatcctc cggcattttc cctgacaccg
    12541 gacgggcggc tgacggcgaa aaatgccgat atcagcggta acgtgaatgc gaactccggg
    12601 acgctcaaca acgtcacgat taacgagaac tgtcgggttc tgggaaaatt gtccgcgaac
    12661 cagattgaag gcgatctcgt taaaacagtg ggcaaagctt tcccccggga ctcccgtgca
    12721 ccggagcggt ggccatcagg aaccattacc gtcagggttt atgacgatca gccgtttgac
    12781 cggcagattg ttattccggc ggtggcattc agcggcgcta aacatgagaa agagcatact
    12841 gatatttact cctcatgccg tctgatagtg cggaaaaacg gtgctgaaat ttataaccgt
    12901 accgcgctgg ataatacgct gatttacagt ggtgttattg atatgcctgc cggtcacggt
    12961 cacatgacac tggagttttc ggtgtcagca tggctggtaa ataactggta tcccacagca
    13021 agtatcagcg atttgctggt tgtggtgatg aagaaagcca ctgcaggcat cacgattagc
    13081 tgaattttat aacccagata cgggcgccag aaatggcgcc ttttttattg cagaaaagcg
    13141 agaggtaatt atgcgtaaat tatgtgctgt tattttgtcc gcagtagtct ggcaggtcgc
    13201 cgctgctacg ccagcgagtg cagcagaaca tcagtccacg ctgagcgcgg ggtatctcca
    13261 tgcctcgacg aacgttcccg gtagtgatga tctgaacggg attaacgtga aataccgtta
    13321 tgagtttacg gacgcgctgg ggctgattac gtccttcagt tatgccaatg ctgaggatga
    13381 gcaaaaaacg cgctacagcg atacccgctg gcatgaagat tccgtgcgta accgctggtt
    13441 cagcgtgatg gcggggccgt ctgtacgcgt gaatgaatgg ttcagcgcgt atgcgatggc
    13501 gggtgtggct tacagccgtg tgtcgactt
//
LOCUS       DACADF010000127        13397 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.129, whole genome shotgun sequence.
ACCESSION   DACADF010000127 DACADF010000000
VERSION     DACADF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.129"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            38..580
                     /gene="ssb"
                     /locus_tag="ISB30_003613"
     CDS             38..580
                     /gene="ssb"
                     /locus_tag="ISB30_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP1991206.1"
                     /translation="MAARGVNKVILVGHLGQDPEVRYMPNGGAVVNLTLATSETWRDK
                     QTGEMRENTEWHRVVMFGKLAEVAGEYLRKGAQVYIEGQLRTRNWQDDAGVTRYVTEV
                     LVGQNGTLQMLGGRRESGVPESAAQPQNPATPAQPAQAAAKSPKAKGGKKGRQDAAPS
                     QQPPQPLPDDFPPMDDDAPF"
     gene            629..877
                     /locus_tag="ISB30_003614"
     CDS             629..877
                     /locus_tag="ISB30_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP1991207.1"
                     /translation="MDHNVLSPLVSLPDGTFTREQAQVVAAQYQNVAIEDDQGTHFRL
                     VVRHQDDGSMIWRVWNFEPGGEDMMNRYIRDYGVRKTK"
     gene            947..3004
                     /locus_tag="ISB30_003615"
     CDS             947..3004
                     /locus_tag="ISB30_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP1991208.1"
                     /translation="MSATESKVKTAPKTSKKTLKSAEAEALKVALDAAQVEYVPVTAL
                     VKSPLNVRTIPYPAEKVCSMADSIEAIGLLQNLVVHNLPDGRCGVAAGGRRLKALQLL
                     QSENRIDAGYQVMVKKVPDELAVAASMAENEQQMAMHPSEQIAGFRTLAEQGKTAAQI
                     GDLLGFGTRHVQRMLKLTELAPEILAALAKDEITTEHCQALALESDQKRQVEVLESAR
                     KRSWNNEASVSSIRNLIISEEVSTNGDKFRFVGEAAFSPDEIRVDLFSSENGGYVKSA
                     SLDTALLEKLQNIAEHLREAEGWSWCDGRLDPISHYGKDTKIWRLHAVPPVEYTEAES
                     ERLAELEALEAKYEDENPGVNDDVVAGALEAVWEEQQTIAHRAKHRAWTDEMKQSAGV
                     VVSWTGQEVKVQRGVVLCADEKMEEKDASTDQAPEKVDPLDAVSVPLLTRLSSERTLA
                     VQAALLQQPQKAVALMVWKMCNSVFHTTTSVKEPFCISVSVSHYALTREAPDGENSVA
                     FQAIQSEKERLEALLPENWRKDMTTFFTLDGATLMALMAFCTACSIDGVQGKDESGRK
                     HQSSLDGVENAIQFDLRDWWKPTADNLFSHMKLPHIVQALSQAGLAGAAQDAAKMKKK
                     DAAEHAEHFLSKIRWVPEWMTSADNQKQPAAKSELSLATSQNDTDADAGDVTDHNNPA
                     CAA"
     gene            3049..3480
                     /gene="psiB"
                     /locus_tag="ISB30_003616"
     CDS             3049..3480
                     /gene="psiB"
                     /locus_tag="ISB30_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HAP1991209.1"
                     /translation="MKTLLTLDVLKTMSSDELEDYRAAGEDFRRELSHAVMRDLTSPS
                     GWSVNAEYRCEFGGFFPVQIRFTPSHGHFDVAVCSPGELNPRWIVVFVTRDGQPFSVV
                     RVMDAFNPELITHTLDLIECLDAGGYSFASIISTLSQEGAQ"
     gene            3477..4205
                     /locus_tag="ISB30_003617"
     CDS             3477..4205
                     /locus_tag="ISB30_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HAP1991210.1"
                     /translation="MIPSSSALVSLKPARQAALQAIMTVEEARQRGARLPSMPHVRTF
                     LRLLTGCSRINSDVARRIPGLHRDPKDRLSSLKQVEEALDMLISSHGEYCPLPLTMDV
                     QAELFPEVLHTRTVRRLKRQDFAFTRKMRREARQVEQSWLLRQNLLGQAVTELNFQSP
                     ETVCTWYTRWSDEFDAAELAAPFWRWQSRFTSLKELDWLRISGEPLYAVMYEIPLIVR
                     ETPEHIRVAERWQVPNKLADRSGV"
     gene            4202..4528
                     /locus_tag="ISB30_003618"
     CDS             4202..4528
                     /locus_tag="ISB30_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HAP1991211.1"
                     /translation="MKWRYSLRWKLPHRPCPGPRELISVVVEAGQAAPEEVMSRWVAG
                     SGYAVCVDFHGQKQIQRWSDERKAAVRRRNMQARIHRVAPLFADELIERELAARPEYF
                     NGKSAR"
     gene            4584..4958
                     /locus_tag="ISB30_003619"
     CDS             4584..4958
                     /locus_tag="ISB30_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991212.1"
                     /translation="MRYWLLVILMNVLFWLIVKHVAPEWYGSPCYYAGQIICTLIAAV
                     ITWRDPPDWLDGILLGVIEFGVLIAGIIFFVWGIGYKLAFWGQHDPNTIYASANDVIL
                     PNLALGTGVLIAGWVYIRYLKR"
     gene            5186..5344
                     /locus_tag="ISB30_003620"
     CDS             5186..5344
                     /locus_tag="ISB30_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP1991213.1"
                     /translation="MKLPRNTLLGCVLIVCLTLLIFTYLTRKSLCEIRYRDTNREVAA
                     FLAYESAK"
     gene            5413..5577
                     /locus_tag="ISB30_003621"
     CDS             5413..5577
                     /locus_tag="ISB30_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991214.1"
                     /translation="MKGLPFTGSPFFLLTGKKARTTGGQKSFIYLQHRFNESQVTLTS
                     FIHRNTLSSV"
     gene            5654..5989
                     /locus_tag="ISB30_003622"
     CDS             5654..5989
                     /locus_tag="ISB30_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991215.1"
                     /translation="METATAELKDHESVRLSKGSPGKPGATSGCIALNPPACRVFLPE
                     KCCYNLFVQCAVSAFLVNQSPGLPGDTAVHSLYDTVMFACFLSRLFLLIMYDICHTVD
                     CVMNRNCST"
     gene            6037..6309
                     /locus_tag="ISB30_003623"
     CDS             6037..6309
                     /locus_tag="ISB30_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991216.1"
                     /translation="MNFTIKSRKTGEIFSFYAPDSGGYVHLESPGHPGNTGAQICRGG
                     GFMGSTLSCGASEDDLASVARKWYRQFVRERRKFLIMSGQYSEDNQ"
     gene            6306..6656
                     /locus_tag="ISB30_003624"
     CDS             6306..6656
                     /locus_tag="ISB30_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991217.1"
                     /translation="MKKEISRNPSFTPSPKLRAHLNSHREGVTERLNNIFDRYAHLVR
                     ACALPLDDDETQVLLNVLNGSVVEPAFIEYLAQEIRDSDDYLEGIPAAKSLYEKCQSA
                     TYPQLLATVERLDR"
     gene            6671..6988
                     /locus_tag="ISB30_003625"
     CDS             6671..6988
                     /locus_tag="ISB30_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991218.1"
                     /translation="MSDVLSCRQLTANLKMIAGAIGCLNRNDVAQIISLGGVPCSKSR
                     ADSIIRSAGAEKNASGNSHLRGARIKRSADVTPEEFNAFCAGLKTFLVSFETNNVSEN
                     NDK"
     gene            7118..7252
                     /locus_tag="ISB30_003626"
     CDS             7118..7252
                     /locus_tag="ISB30_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991219.1"
                     /translation="MLIVLDSSIGSARYFSAGFCSYIPPFLPVMALFPAVSLFKTEKD
                     "
     gene            7396..7572
                     /locus_tag="ISB30_003627"
     CDS             7396..7572
                     /locus_tag="ISB30_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991220.1"
                     /translation="MSEVRVNGSACCDRSGRAAFTVPAIKLPALCRATPFRARALRRC
                     GGCLLLSPGAVSPV"
     gene            7708..7824
                     /locus_tag="ISB30_003628"
                     /pseudo
     CDS             7708..7824
                     /locus_tag="ISB30_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            7829..8185
                     /locus_tag="ISB30_003629"
     CDS             7829..8185
                     /locus_tag="ISB30_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991221.1"
                     /translation="MSTQNVNVKPSTKESTTLRTDGFVRNIHSRNPFDVIRADVVLER
                     IEKEAGWCCGMHYELYQAHLLGGALDYLDALPLKDRPVLMGAAAKRGYLLTLAEEERA
                     LEARDVLMSELAANDC"
     gene            8246..8458
                     /locus_tag="ISB30_003630"
     CDS             8246..8458
                     /locus_tag="ISB30_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991222.1"
                     /translation="MKVYGHQHSSEVFSRETSPSCFEPKAEKLIAFHGKDASYAYNVL
                     ADLKAAGASYTRRNIIADMFRRLGVE"
     gene            8469..8693
                     /locus_tag="ISB30_003631"
     CDS             8469..8693
                     /locus_tag="ISB30_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991223.1"
                     /translation="MEHVKRQIQQGAGNAYYAGMQWRGITLGHADAAALNQFDIDISK
                     AQAPEARTWLLQNKAEFIALMLGIEIVKIG"
     gene            8774..9094
                     /locus_tag="ISB30_003632"
     CDS             8774..9094
                     /locus_tag="ISB30_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP1991224.1"
                     /translation="MFTVIFHDEAEKEFTALPAAIRAKMARLLMKLEANPRQLREPDT
                     KPLGNGLFEIRTMGADIARGIWVYQSGERIFLLRIFIKKSPKTPPAEIDLALRRLEEM
                     QNEI"
     gene            9084..9362
                     /locus_tag="ISB30_003633"
     CDS             9084..9362
                     /locus_tag="ISB30_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1991225.1"
                     /translation="MKYKTLSQVHTEAMNDHEYSAAFEAEEASELLRETLATWRKEAG
                     LTSAQVAERMGIKAPTISRMEKNASRMSIQTLVRYARACGVNFKIQQV"
     gene            complement(9366..9581)
                     /locus_tag="ISB30_003634"
     CDS             complement(9366..9581)
                     /locus_tag="ISB30_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991226.1"
                     /translation="MRAARQRGPVFPAPQARGLSRRGQTGTDAPFACSVPESVNKHRD
                     RDLKSICQRPHVIEGQTAFTTEDPADE"
     gene            9847..10188
                     /locus_tag="ISB30_003635"
     CDS             9847..10188
                     /locus_tag="ISB30_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991227.1"
                     /translation="MLRPSRSRRFHRAGEGLPALCRATPFRARALRRCGGCLLLSPGT
                     RITRLSPFAGAETSTTRLAVQGTAEPFAINFSRPFRCASLDRKKFPAHPRTSAPGRAR
                     LRPRGERLSNG"
     gene            10185..10271
                     /locus_tag="ISB30_003636"
     CDS             10185..10271
                     /locus_tag="ISB30_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991228.1"
                     /translation="MKVISRGQVQQVPAQSQNRGPGNSTGDG"
     gene            10299..11120
                     /locus_tag="ISB30_003637"
     CDS             10299..11120
                     /locus_tag="ISB30_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAP1991229.1"
                     /translation="MRLASRFGLTHSIRQERPLTNDELVKVVPSVFSEEKHNSRSDRY
                     TYIPTITLLDKLREEGFQPFFACQSRVRDEDKRGHTKHMVRLRREGANKGTEVPEIIL
                     LNSHDGSSSYQMIPGMFRFVCTNGLVCGTSFGEIRVPHKGDIVGRVIEGAYEVLGIFD
                     KITEGVDVMKSIALTKEEQRLFGQAALTYRYEDENKSPVSIEQIIHPRRYEDKKDDIW
                     TTYQRVQENLIKGGLPGRTEKGKRTTTRPVKAIDGDVKLNKALWLIAEKFRTLKG"
     gene            11153..11482
                     /locus_tag="ISB30_003638"
     CDS             11153..11482
                     /locus_tag="ISB30_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991230.1"
                     /translation="MVGILMFEFKAVCISISHMEEGDIRVLQVLSSTTNYLTCDSYLE
                     WIHDTPYGCVIRMYDFKRHLLALKRGGVSKFLRKTLILMHREGGYLLFHFDSDADYYP
                     GIDAVDF"
     gene            complement(11515..12000)
                     /locus_tag="ISB30_003639"
     CDS             complement(11515..12000)
                     /locus_tag="ISB30_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP1991231.1"
                     /translation="MKGVAFGIILSLIPLSSMAADCFDMAGRDYKIDPDLLRAISWQE
                     SGFKIDAIGRNPVTGYGSGLMQIDSQHFNELSRYGIKPDHLLSDACLNIYTGAYYLAQ
                     AFKKWGVSWDAVGAYNAGFKKTPRQAARRYEYAKKIHYYYTAIKASKRTSSKDQKIAM
                     N"
     gene            12391..12807
                     /gene="traM"
                     /locus_tag="ISB30_003640"
     CDS             12391..12807
                     /gene="traM"
                     /locus_tag="ISB30_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HAP1991232.1"
                     /translation="MFDAVFNKGTDMARVQAYASDEVAEKINAIVEKRRAEGAKEKDV
                     SFSSVASMLLELGLRVYEAQMERKDSGFNQTEFNKILLENVMKTQFTVSKLLAIGSLS
                     PHVTGDERFEFRSMVSNIREDAKDVISHFFPEQEEE"
     gene            13007..>13397
                     /locus_tag="ISB30_003641"
     CDS             13007..>13397
                     /locus_tag="ISB30_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HAP1991233.1"
                     /translation="MCAKDRSHIAVRSQTDSIHSMREMLNISSCPAFIRDSRGEVIHT
                     SPTFDKIFLTSGGTGAFDTPRAPGSWFLDLSLEIKLELMQSELKSFSEGSAVLVKNIW
                     LAGSLWTVFIETFSINEDTYSKWVFINE"
BASE COUNT         3352 a         3316 c         3535 g         3194 t
ORIGIN      
        1 ttttaaaagg caaaacaacg gattaaggag attcatcatg gcagcacgtg gcgttaacaa
       61 agtcattctg gttggacatc tgggacagga cccggaagtg cgttacatgc ccaacggggg
      121 cgcagtggtc aatctgacgc tggccacgtc tgaaacatgg cgtgacaagc agacgggaga
      181 gatgcgcgaa aacaccgagt ggcaccgtgt tgtcatgttc ggcaagctcg cggaagtggc
      241 gggtgaatac ctgcgtaaag gcgcacaggt ctacatcgag ggccagctgc gcacccgcaa
      301 ctggcaggac gatgccggag tcacgcgcta cgtcaccgag gtgctggtcg ggcagaacgg
      361 caccctgcag atgctcggcg gccgccgtga atccggagtg cctgagagcg ccgcgcagcc
      421 gcaaaatcct gccaccccgg cacaacctgc tcaggccgca gctaaatccc cgaaagcgaa
      481 gggcggtaag aaaggccgtc aggatgccgc cccgtctcag caaccgcctc agccactccc
      541 ggatgatttc ccaccaatgg atgatgacgc gcccttttaa accacgatga agacgccccg
      601 ttcaggcggg gcagaaaagg agaaacacat ggaccataac gttttgtccc cgcttgtatc
      661 cctgccggat ggcacgttta cccgcgagca ggctcaggtt gtcgcagcgc agtatcagaa
      721 tgtggcgatt gaagatgacc aggggaccca ttttcgcctt gtcgtccgtc atcaggatga
      781 cggcagcatg atctggcgcg tctggaattt tgaaccgggc ggcgaggaca tgatgaaccg
      841 ctacatccgg gactacggag tacgcaaaac gaagtaagca gtaagaagaa ggccggacag
      901 gtgggctgtc cggcagacat tcatcaatag ctaagaggta tcaattatgt ctgcaacaga
      961 gtctaaggta aaaactgcac cgaaaaccag caaaaaaacg cttaagtctg ccgaagctga
     1021 ggcgcttaaa gtggctctcg atgccgcaca ggtcgagtat gtcccggtga cggcactggt
     1081 gaagtctccc ctgaacgtca ggaccattcc ctatccggcg gagaaagtct gttcaatggc
     1141 agattccatt gaagccattg gcctgttgca gaatctggtc gtgcataacc tcccggatgg
     1201 tcgctgtggt gtggctgccg gtggtcgtcg cctgaaagcg ctgcagctcc tgcagagtga
     1261 aaaccgtatc gatgccggat atcaggtgat ggtgaaaaag gtacctgatg aactggctgt
     1321 tgcggcatca atggcagaaa acgaacagca gatggcaatg catccgtctg aacagatcgc
     1381 cgggttccgc acactggcag agcaggggaa aaccgccgcg cagattggcg atttgcttgg
     1441 gttcggtacg cgtcatgttc agcgcatgtt gaagctgacc gaactggctc ccgaaattct
     1501 ggcggcgctg gcgaaggatg aaatcaccac cgaacactgc caggcactgg cgctcgaaag
     1561 cgatcagaaa cgccaggtgg aagtgctgga atccgcccgc aaacggtcct ggaataatga
     1621 agcatcagtt tcctcgatcc gtaacctcat tatctcagaa gaggtttcca caaacggcga
     1681 caaattccgc tttgtgggag aggccgcatt cagtccggat gaaatccggg ttgatctctt
     1741 cagcagcgaa aacggcggct atgtcaaaag tgcatcgctc gataccgccc tgctggaaaa
     1801 actgcagaac atcgcggagc acctccgcga agcggaaggc tggtcatggt gtgatggtcg
     1861 tcttgacccg atttcacact acgggaagga cactaaaatc tggcgcctgc atgctgtccc
     1921 gcctgtggaa tataccgaag ccgaatcaga acgtcttgca gagctggaag ctctggaagc
     1981 taagtatgaa gacgagaacc ccggcgtgaa tgatgacgtc gtggccgggg ctctggaagc
     2041 cgtctgggaa gaacagcaga ccatcgcgca ccgggcaaaa caccgggcat ggacggatga
     2101 aatgaagcaa tctgccggag tggtggtttc ctggactggc caggaggtga aagtacagcg
     2161 cggtgtggtg ctgtgcgccg atgaaaaaat ggaggagaag gatgcatcca ccgatcaggc
     2221 ccctgaaaaa gtcgatccgc tggacgccgt cagtgtcccg ttgctgaccc gcctttcttc
     2281 tgaacgcaca ctggcggttc aggcggccct gttgcagcag ccgcagaaag ccgtcgcgct
     2341 gatggtctgg aaaatgtgta actcggtttt ccataccaca acgtccgtca aagagccgtt
     2401 ctgcattagc gtgagtgtgt cgcattacgc gctgacccgt gaagcacctg acggtgaaaa
     2461 cagcgttgcg tttcaggcca tccagtcaga aaaagaacgt cttgaagccc tgctgccgga
     2521 aaactggcga aaggatatga ccaccttctt tacgctcgat ggcgcaaccc tgatggcact
     2581 gatggctttc tgcaccgcct gctccataga tggtgtacag ggcaaggatg aatctggtcg
     2641 taaacaccag agttcgctgg atggggtgga aaacgccatt cagtttgacc ttcgtgactg
     2701 gtggaaaccg acggctgata accttttctc gcacatgaag ctgccacaca ttgtacaggc
     2761 actctctcag gccggactgg ctggcgcagc acaggacgcc gcgaagatga agaaaaagga
     2821 tgccgctgaa catgcagagc acttcctctc aaaaatccgt tgggttccgg aatggatgac
     2881 cagtgctgac aatcagaaac agcctgccgc aaagtctgaa ctcagtctgg caacaagtca
     2941 gaacgatacc gacgctgatg ccggcgacgt caccgaccat aacaacccgg cctgcgccgc
     3001 ctgataacgg agacgccgcc ctgcggggcg gcgataagga gactgaaaat gaaaacgtta
     3061 ctgactctgg atgtcctgaa aaccatgtct tctgatgagc tggaagacta ccgcgccgcc
     3121 ggtgaagact ttcgccggga gctgagtcac gctgtcatgc gcgatctgac ctcaccgtcc
     3181 ggctggtccg tgaatgccga ataccggtgc gaatttggcg gcttctttcc tgtccagatc
     3241 cgttttaccc cctctcatgg tcactttgat gtggcggtat gcagtccggg ggagttgaat
     3301 cctcgctgga tagtggtctt tgttacccgt gacggtcagc cgttctctgt cgttcgcgtg
     3361 atggatgcat tcaaccctga actcatcacc catactctgg atctcatcga gtgtcttgac
     3421 gccggtggct actcgtttgc cagcatcatc agcacgctgt cacaggaggg cgcacaatga
     3481 tcccgtcatc cagtgctctt gtatccctga aaccagcccg tcaggcagcg ctgcaggcca
     3541 tcatgacggt ggaagaggct cgccagcgcg gcgcacgtct gccatcgatg ccacacgtcc
     3601 ggacattcct gcgcctgctg acaggctgta gccggattaa cagtgacgtt gcccgccgta
     3661 ttccgggcct tcaccgtgat ccaaaagacc gcctttccag cctgaaacag gtggaagagg
     3721 cgctggatat gcttatctcc agccacgggg aatactgtcc gctgccgctg acaatggatg
     3781 tgcaggctga actttttccg gaagtccttc atacccgaac agtacgcagg ctaaaacgtc
     3841 aggactttgc cttcacccga aaaatgcgcc gtgaagcccg tcaggtcgag cagtcctggt
     3901 tactgcgtca gaacctgctg ggacaggctg tcacagaact gaattttcag tcaccggaaa
     3961 ccgtctgcac ctggtacacc cggtggagcg acgagtttga cgcggcagag cttgcagcac
     4021 cgttctggcg ctggcagtcg cgattcacat cgctgaagga actggactgg cttcgcatca
     4081 gcggtgagcc gctgtatgca gtgatgtacg aaattccgtt aattgtccgg gaaacacctg
     4141 agcatattcg tgttgctgaa cgctggcagg tgccaaataa gctggctgat cggagtggtg
     4201 tatgaagtgg cgctattctc tgcgctggaa actgccgcac aggccctgcc cgggtccacg
     4261 ggaactgatt tccgttgtcg tggaggctgg gcaggcggca cctgaagagg tgatgtcacg
     4321 ctgggtggct ggctcaggtt acgctgtatg cgtggatttt cacggccaga aacagatcca
     4381 acgctggagc gatgaacgga aagctgcggt gagaaggcgc aacatgcagg ctcgtattca
     4441 tcgtgttgcc cctttgtttg ccgacgagtt aatcgagcgt gaactggctg cgcgtccgga
     4501 gtatttcaat ggcaagtccg cccgatgaat aagtacaatg ctgtctgaca actcagatgg
     4561 ccaaatacaa ggaaagcaga actatgaggt actggttgct cgttatctta atgaacgtgc
     4621 tgttctggtt gatcgtgaag catgttgctc cggaatggta tggttcccct tgttattatg
     4681 caggccagat tatctgcaca ctaatagctg cggtcatcac atggcgtgat ccgcctgact
     4741 ggttagatgg aatcctgctc ggcgttatag aattcggcgt tctgattgcc gggattatat
     4801 tctttgtctg gggcatcggg tacaagctgg cattctgggg gcaacatgat cctaatacca
     4861 tatatgcaag tgcaaacgat gtgattttgc ctaatcttgc gctgggcacg ggcgtcttga
     4921 ttgcgggatg ggtatacatt agatatttaa agcgctaagc agcataaagc gtggatatac
     4981 ctggtaagaa atagcaccgt cccggtataa tcgacgtggc gcttgaggct ttttgcctca
     5041 tgacgtgaag gtggtttgtt accgtgttgt gtggcaaaaa gcagaaagcc ccgtagttaa
     5101 ttttcattaa cccacgaggc cacctgtatg cctagacaac atcaggatag cctcttagat
     5161 gccgcaaggc aaggagaaga aggccatgaa actgccgcga aacactctgc tcgggtgcgt
     5221 attaatagtg tgcctcacac tgctgatatt cacttacctc acccggaaat cactttgtga
     5281 aatccggtac agggacacga acagggaggt ggcggctttt ctggcttacg aatccgctaa
     5341 gtagctaacc ccggcggggg gaagcccccg ccattcaggg gagatgatgt ctgacaagcc
     5401 tgaagcgcct ttatgaaggg gttaccgttc accggtagcc cctttttcct gcttacagga
     5461 aaaaaagccc gcacgacagg cgggcaaaaa tctttcatct acctacaaca cagattcaat
     5521 gaatcgcaag tcacattaac atccttcatt caccgtaaca ccctgtcatc agtctgaaaa
     5581 aaaagtgatt gattgtgaac atttgggttc tggtgataag ttctctctgt agcgtagccc
     5641 tctttacgga ggggtggaaa cagcaacggc agagttaaaa gaccacgagt cagtgaggct
     5701 gtccaaaggc tccccgggga aacccggcgc tacatcaggt tgcatagcac ttaacccgcc
     5761 cgcctgccgg gttttccttc cggaaaagtg ctgctataac ttattcgtgc aatgcgcggt
     5821 atccgctttc ctggtaaatc agtcgcccgg tctgccaggt gatactgcag ttcattcctt
     5881 atatgataca gtgatgttcg cctgcttttt aagcaggctt tttttgttga ttatgtatga
     5941 catctgtcat acagttgatt gcgtaatgaa cagaaattgt tccacttaac tcagtgaggc
     6001 gaagcgcagt cgcgggtgag ccaaaggaga gccagcatga acttcacgat aaaatcccgt
     6061 aaaaccggcg aaattttcag tttttacgca cccgacagtg gtggttatgt tcacctggag
     6121 tccccggggc accctggtaa caccggcgcc cagatttgcc gtggtggagg gtttatgggg
     6181 agtacgcttt cctgtggcgc ttctgaggac gatctggcca gtgttgcccg taaatggtac
     6241 cgacagttcg tgagggagcg gcgcaaattc ttaattatgt cgggccagta ttctgaggat
     6301 aaccagtgaa aaaagagatc tcccgcaacc cctctttcac accttcgcct aagctgcgag
     6361 cacatctgaa ctcccacagg gaaggggtga ctgaacggct gaataatatc tttgatcgct
     6421 atgcgcatct ggttcgcgcc tgtgcccttc ctctcgatga cgacgaaaca caggttctgc
     6481 ttaatgttct gaatggctcc gttgtggaac ccgcctttat tgaatatctg gctcaggaaa
     6541 ttcgggactc ggatgattat ctggaaggga tacccgcagc taaatcactg tacgaaaagt
     6601 gtcagtcagc gacctatccg caattgctgg ccaccgtcga acggctcgat cgctgaagaa
     6661 ggtaaatccg atgagtgacg tactttcctg cagacaactt acggctaatc ttaagatgat
     6721 tgccggggcg attggctgcc tcaacaggaa cgatgttgca caaattatca gcctgggcgg
     6781 tgttccatgt tcaaaaagcc gggccgactc gataatccgc agtgccgggg cggaaaaaaa
     6841 tgcatccggt aattctcatc ttcgcggagc cagaataaaa cgatccgcag atgtcacacc
     6901 ggaagaattc aatgcgtttt gtgcagggct gaaaacattt cttgtctcgt ttgaaacgaa
     6961 taatgtgagt gaaaacaatg ataagtaagc tcctgacctg tctgtgctcc ggacaaatgc
     7021 agtttcagga attaataaat ccctatatat ggcagcgtta attttgtgac gcggattatt
     7081 acatccagcc cgcttattga gctgtagata ttgatttatg ttgattgttc tcgattcgtc
     7141 cattggatca gcccgttatt tctctgcggg cttttgttct tatattcctc cgttccttcc
     7201 tgtgatggct ttatttcctg ctgtgtccct ttttaaaact gaaaaggatt aaaagcacac
     7261 ccacactgga caccttcggc tcctggccag ctgcgctgtc cagtcgtcga gccacactcc
     7321 gccagggcag ctgcttcgca cccgccgggc tccgccgaac ccgcaagcgg tttcgggcct
     7381 gacggctagt gtctgatgtc tgaggtcagg gtcaacggca gcgcttgctg cgaccgttcc
     7441 ggccgcgccg ctttcaccgt gccggcgata aagctgccgg cactctgcag ggcaacgccg
     7501 tttcgcgccc gcgctcttcg tcgttgcggc ggatgcctgc tcctttcccc tggcgccgta
     7561 tcacccgttt gagtccattc gccggggctg agacaagcac agtcgccatc atccgtccag
     7621 ggtaaatggc acgcggtaaa ccgcgccctg gacagctgct gtcgcccgtg ctcgtttacg
     7681 ccctcgcggc gaacggctct caaacgggtg aaggttttat ccagggggca ggttcagcag
     7741 gtccccgcac aaagtcaaaa ccgcggcccc ggcaactcaa cgggtgatgg ttttaaaagg
     7801 cagaaaaaca gacaaaggag aaagctggat gagcacacaa aacgtcaacg taaaaccctc
     7861 caccaaagaa agcacaaccc tccggactga cggttttgtg cgcaacattc attcccgaaa
     7921 cccgttcgat gttatccgcg ccgatgtagt gctggaacgt atcgagaaag aggctggttg
     7981 gtgctgcggg atgcattacg agctgtatca ggcccatctg ctgggtggtg cgctggatta
     8041 tctggatgcg ctgccgttga aagaccgtcc ggtgttaatg ggcgctgcgg caaagcgcgg
     8101 ctatctgctg acgctagccg aagaggaacg cgcgctggaa gcacgggatg tactgatgag
     8161 tgaactggcg gcaaacgact gctgagaaat aaacacggat ggcggcgtat cgctgccatc
     8221 ccttaaagac cagaggtaac gcaaaatgaa agtatacggc catcaacact catcagaggt
     8281 tttctcccgg gaaacttccc catcctgttt tgaaccaaaa gcggaaaagt tgatcgcttt
     8341 tcatggaaag gatgccagtt acgcctataa cgtgctggcc gatctgaaag ccgcaggtgc
     8401 ttcatatacg cgccggaata tcatcgccga tatgttccgc cgtctgggtg tggaatgagg
     8461 tctgggcaat ggaacacgtg aaaagacaaa tccagcaggg ggcagggaac gcctattatg
     8521 cggggatgca gtggcgcggt attacactcg gacacgccga tgctgcagcg cttaatcagt
     8581 ttgatatcga tatcagcaaa gctcaggcac ccgaagcacg cacctggttg cttcagaaca
     8641 aagcggaatt catagctctt atgctgggta ttgagattgt aaagatcggg tagtgagaag
     8701 ttagctaact tagctcttga gctaagttag cttttaagct aacgtataca catacggacc
     8761 agagattaca ttcatgttca cagtcatctt tcatgatgag gctgaaaaag agtttacggc
     8821 tctgcctgct gccattcgtg caaaaatggc caggctactc atgaagctgg aagcaaaccc
     8881 tcgccagcta cgcgagccag ataccaagcc gcttggaaac ggccttttcg aaatacgtac
     8941 aatgggggca gatatcgccc gcggaatctg ggtatatcag agtggtgaac gtatttttct
     9001 acttcggatc tttatcaaaa aatcgccaaa aacgcccccg gcagaaatag atctggcact
     9061 tcgcagactg gaggaaatgc agaatgaaat ataaaaccct tagccaggtt cataccgaag
     9121 caatgaacga tcatgagtac agcgcggcat tcgaggcaga agaagcctcg gagctacttc
     9181 gggaaacctt agcaacatgg agaaaagaag cgggtttgac gtcggcacag gttgcagaac
     9241 gtatgggcat caaagcgcct actatctcac gtatggagaa gaacgcttcg cgaatgagta
     9301 ttcagacgct ggttcggtac gccagagcct gcggtgttaa ctttaaaatt cagcaggttt
     9361 gaatgtcact cgtcagccgg atcctcagtg gtgaacgcag tctgaccctc gatcacatgc
     9421 gggcgctggc aaatcgattt caaatcccgg tctcgatgtt tgttgactga ttcgggaaca
     9481 gagcaggcga agggggcatc tgtccccgtc tggccgcgcc tcgaaaggcc gcgcgcctgc
     9541 ggcgccggga aaaccgggcc gcgctgacgc gccgcccgca tttcctttcc gcgtcttccc
     9601 tgtttgctgc ctgctgtctg tctgcttcct cttcttctcc ctgctgtgat gcctcttttt
     9661 aacccttaaa ggctttaaaa gcacacccat actggacacc ttcggctcct ggccagctac
     9721 gctgtccagt cgtcgagcca cactccgcta accgcgaaaa gacgcggtaa gctccgccga
     9781 acccgcaagc ggtttcgggc ctgacggcta gtgtctgatg tctgaagtca aggtcaacgg
     9841 cagcgcttgc tgcgaccgtc ccggtcgcgc cgctttcacc gtgccggcga aggactgccg
     9901 gcactctgca gggcaacgcc gtttcgcgcc cgcgctcttc gtcgttgcgg cgggtgcctg
     9961 ctcctttccc ctggtacccg tatcacccgt ttgagtccat tcgccggggc tgagacaagc
    10021 acaacccgtc ttgctgtcca ggggacggca gagcctttcg caataaattt ttcccggcca
    10081 ttccgctgcg cttcattaga ccgaaaaaaa tttcccgctc acccccggac atctgcaccg
    10141 ggccgtgctc gtttacgccc tcgcggcgaa cggctctcaa acgggtgaag gttatatcca
    10201 gggggcaggt tcagcaggtc cccgcacaaa gtcaaaaccg cggccccggc aactcaacgg
    10261 gtgatggtta aaaaattaaa caggtgaagg agaataatat gcgtttagca tcccgatttg
    10321 gacttactca ttccattcgt caggaacgcc cactgactaa cgatgaatta gtcaaggttg
    10381 taccttctgt gttttcagaa gaaaagcata attcacgtag tgatcgctat acctatattc
    10441 caaccattac actgctggat aaattacgtg aagaaggttt ccagccattt tttgcctgtc
    10501 agtcacgagt aagggatgag gataaacggg ggcatactaa gcatatggta agacttcgcc
    10561 gtgaaggggc caataaagga acagaagtac ccgaaatcat tcttcttaac tcgcatgatg
    10621 gatcgtccag ttatcaaatg atcccgggta tgttccgttt tgtatgcaca aatggccttg
    10681 tgtgcggtac gtcgtttggt gagattcgtg tacctcataa aggtgatatt gttggaaggg
    10741 ttatcgaagg tgcttacgaa gttctgggaa ttttcgataa aataactgaa ggcgttgatg
    10801 tcatgaagtc tattgcctta accaaggaag aacagcgtct gtttggacaa gctgcattaa
    10861 cctaccgata tgaagatgaa aataaatctc cggtcagtat tgaacagata attcatccgc
    10921 gccgttatga agataaaaaa gatgacatct ggactacata tcaaagggtc caggaaaatc
    10981 tgattaaagg cggtttgcct ggccgaactg aaaaagggaa aagaacaaca acccggccag
    11041 taaaagcaat cgatggtgat gttaaactca ataaggcatt gtggttaatt gcggagaaat
    11101 tccgcactct taaaggctaa ttataagcgc cgggcagctg tccggcgttt tgatggtggg
    11161 aatacttatg tttgaattta aagctgtctg tatcagtatt tcacatatgg aagaaggtga
    11221 tatcagagtt cttcaggtgc tttcatcaac aaccaattat ctaacttgcg atagttactt
    11281 agagtggata catgatacac cttatggttg tgttataagg atgtatgatt tcaaacgcca
    11341 tcttctggcg ctaaaaagag gtggggtgtc aaaattttta cgcaaaactc ttatactaat
    11401 gcaccgggag ggtgggtatc tgctttttca ttttgatagt gatgccgatt attatccagg
    11461 tatagatgcc gtagattttt aagaaattca agccttctta tatcagaagg ctttttagtt
    11521 catggcgatt ttctgatctt tagaagaagt tcgtttgctt gctttgatag ccgtgtagta
    11581 gtaatggatc ttcttcgcat actcgtaacg tcttgctgcc tggcgcgggg tctttttgaa
    11641 gcctgcattg tatgccccga ctgcatccca cgaaacgccc cactttttaa acgcctgagc
    11701 cagataatag gctccggtgt agatgttaag acaagcatcg ctaagaagat gatccggttt
    11761 tattccatat cgcgacagtt cgttgaaatg ctgcgagtca atttgcatca agcctgatcc
    11821 ataacccgta acaggattac gcccaatcgc atcaatttta aatcctgatt cttgccatga
    11881 tatagcacgc aggagatccg gatcaatttt gtaatccctg ccagccatat caaagcagtc
    11941 tgctgccatt gatgacaatg gtattaaaga caggatgata ccaaacgcta ctcccttcat
    12001 gaattcccct ccgtgccaaa tcccggaata accttaaccc atccaagacc gagactcaac
    12061 atatatgcac tcctcaccac aaaatcacca cacactacgc aaaattaaaa ttttgcagat
    12121 ttcactttag cttcagtggc ttaactgtag ttatattaca ttatgttatt tttctttata
    12181 tatatgtgag ttgccggtga gtcagtagta agtcgtcggt aagtctatag cgactcgttt
    12241 atgactcact cttttataat catatattta aagcgactta actctagact taagtcgcca
    12301 tatgactcgt aatgcaactt tcttagactc tagacttaag tttagagttg taaagaaggt
    12361 aaactcgcgt tatcttctcg ccataataac ttgtttgatg ctgtttttaa taaaggaacc
    12421 gatatggcca gggtacaagc ttatgctagt gatgaagttg cagagaaaat taatgcaata
    12481 gtggaaaagc gaagggctga aggggcaaag gaaaaggatg tcagtttttc aagtgtcgct
    12541 tcgatgctcc ttgaattagg gcttagggtt tatgaggctc aaatggaaag aaaagattca
    12601 gggtttaacc aaactgagtt taataagatt ttgcttgaga atgtcatgaa gactcagttt
    12661 accgtatcca aacttttggc tattggctct ctaagccctc atgttacagg tgatgaacga
    12721 ttcgagttcc gcagcatggt gagtaacatt cgagaagatg caaaagatgt tatttctcac
    12781 ttcttccccg agcaggaaga agagtaagaa ctggtgtgtg tttatggtgg tttgtaatta
    12841 attcagcagt aaaaaaagaa taatgatcta gctttaaaaa gtgacatgga agtataagga
    12901 atatagaata cttacagggt tgtactaaaa tttgttgaag cgttgacgtc cggggcttgt
    12961 ccccccgaac gtcaactatc catagtgtcc tttccaggag gcgcctatgt gtgcgaagga
    13021 tcgtagtcat attgctgtgc gcagtcaaac agattccatt cattctatgc gcgagatgct
    13081 taatatttca tcttgccctg cttttattcg tgattcccgg ggcgaagtaa ttcacaccag
    13141 cccaaccttt gacaaaattt tcttaacatc tggtggaact ggagcatttg atacacccag
    13201 agcaccggga agctggtttc tggatctctc cctggagatc aaactggaac tgatgcagtc
    13261 agaactgaaa tcgttttcag aaggttctgc tgtacttgtt aaaaatattt ggttggcagg
    13321 ttcactttgg actgttttta ttgaaacgtt ctcgattaat gaagatacat acagtaaatg
    13381 ggtttttata aatgaag
//
LOCUS       DACADF010000128        13130 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.130, whole genome shotgun sequence.
ACCESSION   DACADF010000128 DACADF010000000
VERSION     DACADF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.130"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            33..1721
                     /locus_tag="ISB30_003642"
     CDS             33..1721
                     /locus_tag="ISB30_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HAP1991234.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGIPV
                     AERVVGHICISGPSLMSGYFGDQISQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
     gene            1718..2632
                     /locus_tag="ISB30_003643"
     CDS             1718..2632
                     /locus_tag="ISB30_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP1991235.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVTGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQQLTAMADAITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2664..2912
                     /locus_tag="ISB30_003644"
     CDS             2664..2912
                     /locus_tag="ISB30_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP1991236.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2912..4084
                     /locus_tag="ISB30_003645"
     CDS             2912..4084
                     /locus_tag="ISB30_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP1991237.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4119..5198)
                     /gene="lptG"
                     /locus_tag="ISB30_003646"
     CDS             complement(4119..5198)
                     /gene="lptG"
                     /locus_tag="ISB30_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAP1991238.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIFARSASIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5195..6265)
                     /gene="lptF"
                     /locus_tag="ISB30_003647"
     CDS             complement(5195..6265)
                     /gene="lptF"
                     /locus_tag="ISB30_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAP1991239.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGNPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            complement(6296..6856)
                     /locus_tag="ISB30_003648"
     CDS             complement(6296..6856)
                     /locus_tag="ISB30_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAP1991240.1"
                     /translation="MSSRLIIALIIMLLAQGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWRLPEQSSTILVLNKDGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6868..7692)
                     /locus_tag="ISB30_003649"
     CDS             complement(6868..7692)
                     /locus_tag="ISB30_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991241.1"
                     /translation="MMRKYFPLEASERLFVAVEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            complement(7692..9041)
                     /locus_tag="ISB30_003650"
     CDS             complement(7692..9041)
                     /locus_tag="ISB30_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAP1991242.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQIWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9087..9860)
                     /locus_tag="ISB30_003651"
     CDS             complement(9087..9860)
                     /locus_tag="ISB30_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991243.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTQSMKTKSPA
                     LWAAVIMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATG
                     KISMFLAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIG
                     YNGTKILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9892..10605)
                     /locus_tag="ISB30_003652"
     CDS             complement(9892..10605)
                     /locus_tag="ISB30_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAP1991244.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNMTALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIQAALS"
     gene            10779..11471
                     /locus_tag="ISB30_003653"
     CDS             10779..11471
                     /locus_tag="ISB30_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAP1991245.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRSVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNIEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLIDAEK
                     AITDFMTARGYH"
     gene            complement(11520..13019)
                     /gene="pitB"
                     /locus_tag="ISB30_003654"
     CDS             complement(11520..13019)
                     /gene="pitB"
                     /locus_tag="ISB30_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAP1991246.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT         3322 a         3409 c         3245 g         3154 t
ORIGIN      
        1 tgtctaataa aatctttacg cattccctac ctatgcgcta tgccgatttt ccaacgctgg
       61 ttgatgcttt ggactatgcc gctctgagta gcgctggaat gaatttttat gacagacgtt
      121 gccaacttga agatcaactg gaatatcaga cgttaaaagc gcgtgccgaa gctggtgcga
      181 agcggttgtt atcgctgaac ctgaaaaaag gcgatcgcgt ggcactgatt gccgaaacga
      241 gtagcgggtt tgtagaggct ttttttgcct gccagtatgc cggcttagtc gccgtcccgt
      301 tggcgattcc aatgggcgtc ggtcagcggg attcctggag cgccaaactg caggggttac
      361 tggcaagttg ccagcccgca gccattatca ctggtgatga gtggttgcca ctggtcaatg
      421 ccgcgacgca taacaatccc gaattacatg ttttaagcca cgcctggttt aaggcattac
      481 cggaagccga tgttgcgctt caacgtccag ttccaaacga tatcgcctac ctccagtaca
      541 cctccggcag cacccgtttt ccccgtggcg ttatcattac tcaccgcgaa gtgatggcta
      601 atctacgtgc tataagccac gatgggatta aattacgccc tggcgaccgc tgcgtctcct
      661 ggctgccttt ctaccatgat atgggactgg tcggctttct cctgaccccc gtcgccacgc
      721 agctttcagt agattatttg cgcactcagg attttgccat gcgtcctctg caatggctta
      781 aattgatcag taaaaatcgc ggcaccgttt ccgttgcgcc gccgtttggc tatgaattgt
      841 gccagcgccg cgtgaatgaa aaagatctcg ctgaactgga tctttcctgc tggcgcgtcg
      901 ctggtattgg tgcagaaccc atctccgcag aacaactcca tcaattcgct gaatgtttcc
      961 gtcaggtcaa ctttgacgat aaaactttca tgccgtgcta cggactggca gaaaatgcgc
     1021 tggctgtcag cttctctgat gaagcttccg gggttgtggt taacgaagtg gatcgcgaca
     1081 tcctcgaata ccagggtaaa gccgtcgcgc cgggtgctga aacacgcgcc gtatcgactt
     1141 tcgtcaactg cggtaaagcg ttgccggaac atggcattga aatccgcaat gaagcaggta
     1201 tacctgtcgc ggaacgtgtg gtaggccata tttgcatctc cggccctagc ctgatgagcg
     1261 gttactttgg cgaccagatt tcacaagacg agattgccgc gacgggctgg ttagacaccg
     1321 gcgacctcgg ttatctgctg gacggttatc tgtatgtcac cggacgcatt aaagatctga
     1381 ttattattcg tggccgtaat atctggccgc aggatattga atatattgcg gaacaggaac
     1441 cggaaattca ttctggcgat gcgattgctt ttgttaccgc ccaggaaaaa atcattttgc
     1501 agatccagtg tcggatcagc gatgaagaac gtcgcgggca gcttatccac gcgctggcag
     1561 cacggatcca aagcgaattt ggcgttaccg cggatatcga tctgttgccg ccccacagta
     1621 ttccccgaac atcctccggc aagcctgccc gtgcggaagc gaaaaaacgt tatcagaagg
     1681 cttatgctgc cagtcttcat gtgcaggagt ccctggcatg aatcaaactg tcgcggtgac
     1741 gggcgctacc gggtttatcg gtaaatatat tattgataac ctgctcgccc gcggctttca
     1801 cgttcgcgca ttgacgcgta ctgcccgcgc tcacgtcaat gataatctca cttgggtgcg
     1861 cggttcgctg gaagatacac attcgcttag cgaactggtt accggagcca gcgcggtggt
     1921 ccattgcgcc gggcaagtgc gcgggcacaa agaagagatt tttacccgct gcaacgttga
     1981 cggcagcctg cgcctgatgc aagcggcaaa agagagcggc ttttgccaac gatttctgtt
     2041 tatctcttcg ctggcggcgc gccatcccga actctcctgg tacgcaaatt ccaaacacgt
     2101 cgccgaacaa cagctgactg caatggctga cgcaattacg ttgggcgttt ttcgcccgac
     2161 agccgtgtat ggtcccggcg ataaagagtt aaaaccgctg tttgactgga tgctgcgcgg
     2221 tctgctgcca cgacttggca cgccagagac acagctctct ttcctgcacg tcaccgattt
     2281 tgcgcaagca gtgggtcagt ggttaagcgc cgaaactgta cagacgcaaa cctatgaatt
     2341 atgcgatggc gtcgccggag gctatgactg gcaacgtgtc caacagcttg ttgccgatgt
     2401 ccgctgtggt tccgtgcgga tggtcggtat tcctctgccg ctactcacct gccttgcgga
     2461 tatcagtacc gcgttgagcc gtctggcggg taaagaaccg atgctgactc gttcgaaaat
     2521 ccgtgaatta acccatgccg actggtcggc aagtaataac cgtatttctg aagatattaa
     2581 ttggtttccc gggattagcc tggaacacgc attacgcaac gggctatttt gattgtcttg
     2641 tttaaaggtg tgttaacgtt tctatggtaa atcgtgaaat agtaatggat tacatccttt
     2701 cctgtttaca ggatttggtc gaaaacggtg tggaaattaa gcccgacagc gatctcgtca
     2761 acgatcttgg cctggagtcg atcaaagtga tggatctctt aatgatgctg gaagacagat
     2821 tcgatatttc cattcctatt aatattctgc tggatgtcaa aacccctgcg caattaatgg
     2881 aaactctact tccatggctg gagaataaat aatggggcta tacgataaat atgcgcgcct
     2941 tgctggcgag cgactgcaat ttagtgacaa cgggttaaca ccgtttggca cctgtattga
     3001 tgaagtgtat tccgccaccg aaggtcggat cggcaataaa aaagtgattc tggccgggac
     3061 caataattat ctcggtttaa cctttaatca tgatgccatc gccgaaggtc aggcagcgtt
     3121 agcggcccaa ggcaccggca ccaccggttc tcgtatggca aacggcagtt atgcgccgca
     3181 cctggcgtta gagaaagaga ttgccgagtt tttcaatcgc cctaccgcta tcgttttttc
     3241 aaccggttat accgctaatt taggcgtcat cagcgcactt gccgaccata acgccgtggt
     3301 gttactggat gccgacagcc acgccagtat ttatgacgcc tgttctctcg gtggcgcaga
     3361 aattattcgt ttccgccata acgatgctaa agatctggaa cggcgcatgg ttcgcctcgg
     3421 tgaacgggcg aaagaggcga ttatcatcgt tgagggcatc tacagcatgc tcggtgacgt
     3481 tgcgccgtta gcggaaattg ttgatatcaa acgtcgtctc ggtggttatt tgattgtcga
     3541 tgaagcgcat tcgtttggcg tgttaggcgc aacggggcgc ggactggctg aagccgttgg
     3601 cgttgaagat gatgtggata ttatcgttgg tactttcagt aaaagtctgg cctcgattgg
     3661 cggttttgct gtcggttcgg aagcgatgga agtgctgcgc tacggcagcc gcccgtatat
     3721 ttttaccgcc tctccttctc cgtcctgcat tgcgaccgtg cgctcgtcgt taagaaccat
     3781 tgccacacaa ccagagctgc ggcaaaaact gatggataac gccaaccatc tgtatgacgg
     3841 tctgcaaaaa ctgggttatg agttaagttc acatattagc ccggtagtgc cagtaataat
     3901 cggttcgaaa gaggaaggtt tacgcatctg gcgcgagctt atttcgctgg gcgtttatgt
     3961 gaatcttatc ctgccacctg ccgcgcctgc aggcatcact ctgctgcgct gtagcgtaaa
     4021 cgctgcccac agtcgtgagc aaatcgatgc catcattcag gcatttgcta ctctgaagca
     4081 gtaatgtgag cccgctgtgg tgttgcgcca cggcgggttt aatgttgtct gtcataccaa
     4141 tacaccaggc tcaacgccac caacacaaac gctaccggta aaccgagcgc ggtaacaggt
     4201 gcgctcaacg caaataacaa tcccagattg accatgattt gatagctgat ccaggtgagt
     4261 aagccaacga tgacgcctac agcgagacgg ctacccatcc ctggcgagcg cggggcgcta
     4321 aaggtgaacg gcacagccag caaaatcatc gccagggtca ggatcggttg ccccagtttt
     4381 tcccacagtg caaggcggta ttcgctgctg ggttgtccgg tttcctgcaa ataatggatg
     4441 taatgattaa gctgacggac agaaaaagta ttccccggca tcgataactc ttcaagatcc
     4501 atactggtga aggccgattg ccaggcaaga ttatctgatg tttccagcgt ttctttgcca
     4561 ttcaaccatt ttttatgatt cacaccatgc aggatccacg ttttgtcgtc ttcaatggag
     4621 gcactgcgtg caaaaatgta ggattcgagc gagagatcgt cgcgataatg aaatatctcc
     4681 acgcccacag gctggccctg ctcgttcagc gatttcaccg tcacaaattc attaccgcgc
     4741 ctggcccaaa gcatattgcc ggtaatgtca tcgtcctccc ccaacgcggt ggcagtcgat
     4801 ttgatttgca gcgcctgttg ctgcaatggc gacgccaccc actcatctat cgcgcctaac
     4861 gaaacagtcc acaatatccc ggcaaccagc gccaccagtg caatacggaa gatagaaaac
     4921 ccagtgctgc gaatggcggt aagctcactg tttttcgata actgccccag gccaacaatc
     4981 cctcccaata atgcgataaa cggcgaaagt tcgaccagtg tacgcggtaa ggtcatcagc
     5041 accaccagca ccgcctgagt ccagcgataa ccgcccgggc tgacatcatc cagttcgtta
     5101 atcaggttga aggtggtaaa aagcggcagc aatagccctg cggcggcggc aaaaccgaga
     5161 aagagatgac ggattaaata gcgactgaaa acattcatcg ggaaaatttc tgcagcaaag
     5221 agaagtcgcg tgcgaccagc atcagcaggc ccattagcat gagtccgggt actaaccaca
     5281 aaccaggaat gaggggaatc gcaccgttag ccaccagcgt acggcagacg tcgccgccat
     5341 aaaaaatggc aacaaacagc aatgtcagtg gcaataacgt tgaaaatcgc ccttgccgcg
     5401 gttttacccg acttaatgaa atggccagca gcgccatcaa caatgctgtc aggccacggc
     5461 tttgtcgcca ttgcagttcg gcatgatcgg caggaaaggc tgagcgcgcc agctccgtga
     5521 ccgattttgc tttgcgtttg acgttagggc tttgatccag cggattcaga tgtaattgca
     5581 gattacggta gatctgttcg ttgtcgtcac gcccctgatg atcgagaaga taggcggtcc
     5641 cgttatgcaa cataacggtc ggtttctcag gtgatgggtc aaccacatca accgaacggg
     5701 cgcggaaaat gcgggttcga ttggcagtag aagtataaat cagcgcgtca gtcaggcgat
     5761 tattatcctg atcaaccgtc tgcgaaagga tcattcgtcc gttatcgtta gtgttaaatt
     5821 tctttgcccg caactggcga acatccagct ccgactgcga ctgttgctcc agttgataaa
     5881 tctgcgcata agcccacggc cgaccataca tcgacagaag ggtgacaaaa atacttagcg
     5941 gtatcgccag ataaagaacg gctttgtaca agcgtcccgg attgcccccc gccgcagaga
     6001 tagcggtaat ttccgagtcg gtatacatct gccctagcgt cacgccaact gacacataca
     6061 gaccaacagg taacaacatc tccagtgcaa tcagcacttt gtagaaaacg atatccagca
     6121 caacatcaag agctaaggtc ccatttgccg cttcggtcag ataacgctgt gcggagtagc
     6181 tggcgaaaat aaagatcaga aaaccgacga taatcagcac cagacggcgc gtaccacgca
     6241 tgatgtagtg ttcaaccagc ttcatctcac taacaccctc aatcctttga attttttatt
     6301 cattgatcag tttctgcgcc agcgcaatga catgatctac ctcttccggc gtcaggctgc
     6361 cgtcctttgc ccactgaatt tgcccgtctt tattcagcac cagaatggtt gaactctgct
     6421 ccggcaaccg ccaggcaacg cgtcccagtc cattgccgtc aataacgaat tgcgcccacg
     6481 gatagcggcg tttatttttt tcgattttgc caacgacaaa atagccagta ccaaagatgg
     6541 catcatcggt gttcacaatg gtggtgggct gaaaacgatc ctgcggaaag tttgccgctt
     6601 caaccgcttt aattaacagt gagttctttt tcttcgccga cttacgcccg gcgatatact
     6661 ggataatgcg gacttttccc gccagctgtg tgctgttcca cttttggtag tgaatttcgt
     6721 cgtcgctatt cagaagcagc tcgccgcctt cctggatata aacgggggta accgttttgc
     6781 cagtgacaaa attgtgtgcc tgcacaccct gggctaagag catgataatc agcgcaataa
     6841 tcaaacgaga tgacatcatt gctccggtta tagcggaaaa cgcgaaatca gcagctctaa
     6901 gcgctggcga atatcgctat ccagcgccac cggtgcatca tacgactggc ggttttccat
     6961 atcatccgcc accatgtcgt cgtgtttatc ccccatcagg ccattaatgg cggcgaggaa
     7021 gcgggaaatc tgtaacgcct ctttattttc aggatcaagc gatcttaagg tgtcgtaata
     7081 agaaacctgc tggctgatga tttttcgcac atcatgaaat tccctgccgg tgagtcgcgg
     7141 tttggcaatt atctctttca gggtatgcat ttgcttttgc cgataggcaa taaaaccgct
     7201 tacgctttct aactggctat ggcgtaatga atagttcacc aacgaccaga ccggagagct
     7261 taaataaacc cgtaacaagt tgccgtaata cgacacaata tttttcttac cgttacgaaa
     7321 accgtcctga aaacgcccca aaaaaacggt cgttttgccg aataaaaatc cggcctgatg
     7381 cttctttaaa tacagccgtt gcgcaaaacg caggtgcttg tagcgcgctc gcatgtattt
     7441 aaattgtttg cgctcatcag cagtcagctc atcgcctttg gcctgtttgc atattaaacg
     7501 cagtaactcc tggcgattga cgccattgag ccagaattgc aggcataacg cgtaattatc
     7561 atggatgatt tcggtggtac aacttagtgc gatagtcggc ggcaaactga cctgtgcatc
     7621 aacgacatca tcttcttcaa ctgcaacaaa taaacgctcg ctcgcctcaa gcgggaaata
     7681 ttttctcatc atcaggattt ctctccggct gcgttataaa gcaaggaatg gcgctgaaaa
     7741 aatgccttta aggtcggaat taacgagagc accacatcca gacattgccc accgaaataa
     7801 gcaaacgctg ctcccagcac gccaaacaga tgacagaaca tgaacagcaa tacgatgtag
     7861 ccgattgagg cgatggtttg cgccacgaga aaagcgcgct gttttcccgc catcaatagc
     7921 aatgactcct gtgggaagcc cagcatcgaa atcacaattg cgcccagcat cacctgaatc
     7981 aggtcatacg cttcgagata cttaacgcca aacaccagcg aaataagcgg cttgccgacg
     8041 atcaacaccg cgagcgccac cagaatacca attccgcccg ccagtaaacc tgacttcacg
     8101 cctaacaacc acggtctggt ggtgcgcgga tctaaacgca tcacctctgg gtaaaaactt
     8161 ttgcccagta aacccgctgg cgttccggcg gcgtcgaaga atgtcatggc gattttaaat
     8221 aacccggcgg cagcgggtcc taacacgatc cccaccagca ctgtgctgca cgagttacgc
     8281 gccgaccaga tggagtgggc aatgttggtt gaccagacaa aactccacgc gcctttaatg
     8341 tgtcgggcag actcaaacag attcaatttg aaggcgttat ggatatttct gcggcgtaat
     8401 tcgcgcgcgg caaaccacca gtacatggtg ccgccaacca gattcgacac gtaccaggca
     8461 ataacaaaac ccgcaaaacc aaagtcaaaa taccaggcta cgacgctccc cgctgcgcgc
     8521 agaaaaggtt tcgtcgcttg ctgtacagca attagatcga agcgatctac cgcacgcaga
     8581 atgccggtcg gcgtggagga agccattgaa ggaatgagcg tgcaatacag cgctgccagc
     8641 caaaagcttt ggtcatccag acctaatgaa tgggaaagaa aagggagtaa ggcaatgccg
     8701 ccgacaatcg ccaccgcgcc gctgacgata tccagcgaga aggaaaatga gacgacattg
     8761 cggaattgct gcggattatt gttggttaat gctggtgttc cgtactgaac caccagctgc
     8821 catatctgaa acttaataaa atcgctgatc gacttggcgt acgattgtac aatcaccagt
     8881 acgccaaaca tggcgggcgt catcccttta ccggcacacg agagcgccaa cagacccagt
     8941 aaagcgctca cgacattact ggagcctaac caggcgctat tgcgaataat ggtgcgaaac
     9001 gcgccatctg caaaccaatg tttgatgtta aaacccgcca attcagcctg accttatcgt
     9061 tgatagtaaa aagtatcccg ccagccttaa gttaaacttc ggcggtcaga aacgatggca
     9121 accagagaaa ccgccttctg tgcctgttcc agcacttcgc tgtagggcgc tctggaatca
     9181 atctcaagaa tttttgtgcc gttatagcca atcgtcgaca tgacgccgat tttgtcctgc
     9241 agctcggcat agtcatggtc aggcttgcgg gagatggcag tatcaatatc aatccccaaa
     9301 cgaatgatta attcaggacg gtattgcgcc attttttggt ataaacgccg ttcgcgctgc
     9361 gccagaaaca tgctgatttt cccggtcgca cgttcgacgc cgatccccgg tccgtcataa
     9421 taaaagcccg aaatttcagc ctgcgggaag cgatcgctga ccaccagaac gccactttgc
     9481 gccagtcgct gaaccttgcg tagattcgcc attcttcgca gcgagaagca gtacataatc
     9541 accgccgccc atagcgctgg agatttggtt ttcatgcttt gggttttcat gctttgggtt
     9601 ttcgatgatt tggccgccag tcgcctttcg agccagacgc caaccaacgg caatcgtttg
     9661 attttgtcgc cgtcttcgcc cgagagcagc cccagatagc gccgctcggt ttgccagtgt
     9721 tgttgcagcg atttcacgag gtcggtggtc agtgtggatt taccggtgcc atcacacccg
     9781 accaccgcaa tcagccccgg aatgtagttg ggctgcggcg cggttgtact attaacagtt
     9841 tgtgtttgta gtgcgtccat tcgttaaatc atcccctatc cattttggtg atcaggaaag
     9901 cgcagcttga attgcgcgta gtgacgcttg cagaatttca tcggctggtt gccgaccatc
     9961 cagttcaagg atcctggcgc cattaaatgt cagttgcggc gtaacggcga ttttttcctg
    10021 taacgccgcc agttggtggt caggtttacg cgcaaacgcg gtttgttcat caatgccaag
    10081 acgaatcaac aatacgggca aataagatgc catccattgg tacagcttca gctcgcgctg
    10141 ccttaacatt tttatccaac cgttaccgcc cgtggttttt gccaattgcg ggccatcaaa
    10201 gcgaaaaccc ggcacttcaa cctgcgggta gcggtcggtg atgagcagat agccttgctg
    10261 gcttttacac aacattttgc gaaacttata cgcccgccag caggaaagca gatagataac
    10321 cagcgcagtc atattgccag gcggtgtgga gggcttttcg tgcacatgtg ccgctttact
    10381 tcgcagataa cgcccaaaag gtgcgccaat aacagggagc tgtgaaatcc attcgccaat
    10441 tcgcccggac gattgcccga gataaatgtg ttctgttggc attgttgctg ccagttcatt
    10501 taccaggctt gccgtgaggg tcgatttacc tgaaccatca catcctacaa tggcaataac
    10561 acgcactggc gtggaattaa ttattgacat attacgttga ttcacgaaaa acccggcagc
    10621 aaataatgta ttgaatatta cgtggtccgt gctcagaata tccgttcaac cttattgtga
    10681 caatggttaa ttgtgacatt gacctgaatt acgctttcat aaaaacatat taaccaaata
    10741 aatattttta atggatattt aaattaaagg atatattcat gcagtcaata acacctccat
    10801 taattgccgt tattggtagc gatggttcag gcaagtcaac ggtgtgtgaa catctcatta
    10861 ccgttgtcga aaaatatggt gctgccgaaa gagttcattt aggaaaacag gccggaaatg
    10921 tcggtcgttc agtgacaaaa ttaccgttga tgggaaaatc cttacataaa acaattgaac
    10981 gaaatcaggt gaaaacagca aaaaaattgc caggaccagt tccggcgctg gtaattacag
    11041 cgtttgtcgc ccgtcgctta ctgcgctttc gtcatatgct tgcctgtcgt cgtcgcgggt
    11101 taattgttct aaccgaccgt tatcctcagg accaaattcc tggcgcttac gatggtacag
    11161 tgttcccacc taacattgaa ggtggtcgtt ttgtctcatg gctggcaagc caggaacgta
    11221 aagcgtttca ctggatggcg agccataaac ctgatctggt catcaaactc aatgttgacc
    11281 ttgaagttgc ctgcgcgcgt aaacccgacc ataaacggga atcgctggcg aggaagattg
    11341 ccataacgcc acagttaacc tttggcggtg cacaactggt tgatatcgat gctaatcagc
    11401 cactggaaaa ggtgctgatt gatgcagaaa aagcgattac ggattttatg accgcgcgtg
    11461 gttatcacta gtcaaaaatg gaaatgcccg atcgccagga ccgggcattt tcaggaaggt
    11521 taaatcaact gcaatgctat ccagtacaac ccaccagaaa gaaaaatcgc cgccggtaaa
    11581 gtaaacaccc acgccatcag gatgctggtt accgttttac gctgtaaccc accgccgtcc
    11641 accaccatcg ttcctgctac ggcagatgag agtacgtgtg ttgtggagac gggcatccca
    11701 atataactgg caaggccgat agacactgcc gccgtcattt gtgccgccat gccttgcgca
    11761 tacgtcatgc cgcgcttacc aatcttctca ccgatggtca tcgctacacg acgccagcca
    11821 atcatggtgc caatgccgag cgccagtgct actgccatga tgatccatac cggtgcgtac
    11881 tcaatggtgc ttaacatatc gctgcgcagt tttttcagca ggttctggtc ttctttactg
    11941 acgcctggca gtttcgccag cttcgcggaa gtatcagaga tgcacagcat aatgcggcgc
    12001 agctggctgc gctgactcac gcttaacggc tcgtagcttt ccatattgcc tggcagcatc
    12061 gttttaacgc gcgcaatcgc atcaaaggta tttgccggat gacagtgaaa ctccgtcact
    12121 tgcgcgccat cagtcgatgc cgcaggcaat ggaggttcca tcgcaatcaa cttctgcggc
    12181 agttcaggat gctgttgcag gtagtgttcg aagttggtga cggcatcgcg ggtacgggta
    12241 atttcatagc cggacgcgtt catattgacg acgaagccag caggggcaat ccccaccagt
    12301 accagcatta ccaggccgat ccctttctgc ccgtcgttag cgccgtgcga aaacgccacg
    12361 cccgcagcgg aaacaatcag cgcaatacgc gtccagaatg gcggcttacg tttgcctttt
    12421 ttctttttgc gatcttccgg aatgcggtga atacggtcac gctttttcgt cccgctccag
    12481 aaacggcgca gcaggaaaat cagaccaccc gcaatgacca ggccgacgat aggggaaaca
    12541 atcagcgagg agaaaatttt ggtcacttca cgcaggttta acgcatccat caccgatgag
    12601 ccggttaaca gcgcgttggt taaaccgatg ccgataatcg caccaatcaa ggtgtgcgaa
    12661 ctggaggccg gtaaaccaaa gaaccacgtt cccaggttcc agataatcgc cgccagcagc
    12721 atggaaaaga ccatcgccag gccgtgagtt gaccccatat tcagcaacaa atcggttggt
    12781 aacatatgga caatggcata ggcaacgcta agtccgccca ataacacgcc aaaaaaatta
    12841 aaaaatgccg ccatcaccac agcaagttgt ggttgcatgg cacgagtata aataacggtt
    12901 gccaccgcat tcgccgtgtc atgaaaacca ttgattgctt cgtagaacaa cacaaatgcc
    12961 agagcaagta ataacaaaag ccctgtgtat atatcaaggc caacaaataa atttagcata
    13021 tattagatta cgccattttg aatttacgaa cggacgcatt atcagtgact ttaacggcat
    13081 gggcaaagtg aaatatcatt ttttaacgat aaaaagcgtg ttatttcttg
//
LOCUS       DACADF010000129        13083 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.131, whole genome shotgun sequence.
ACCESSION   DACADF010000129 DACADF010000000
VERSION     DACADF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.131"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(82..972)
                     /gene="rarD"
                     /locus_tag="ISB30_003655"
     CDS             complement(82..972)
                     /gene="rarD"
                     /locus_tag="ISB30_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAP1991247.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRISK"
     gene            complement(1024..1491)
                     /locus_tag="ISB30_003656"
     CDS             complement(1024..1491)
                     /locus_tag="ISB30_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAP1991248.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1656..2525
                     /gene="pldA"
                     /locus_tag="ISB30_003657"
     CDS             1656..2525
                     /gene="pldA"
                     /locus_tag="ISB30_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAP1991249.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2650..4479
                     /gene="recQ"
                     /locus_tag="ISB30_003658"
     CDS             2650..4479
                     /gene="recQ"
                     /locus_tag="ISB30_003658"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP1991250.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4544..5164
                     /gene="rhtC"
                     /locus_tag="ISB30_003659"
     CDS             4544..5164
                     /gene="rhtC"
                     /locus_tag="ISB30_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAP1991251.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRCGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5202..6062)
                     /locus_tag="ISB30_003660"
     CDS             complement(5202..6062)
                     /locus_tag="ISB30_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAP1991252.1"
                     /translation="MTEATTPHDAIFKAFLSRPETARDFIEIHLPPSLVKLCNLDTLH
                     LESSSFVEENLRQYYNDVLYSVETTKGCGYIYVLIEHQSSPDENMAFRMLRYAIATMQ
                     RHLEAGHDYLPLVIPILFYQGKRSPYPWSTNWLDGFPDPDIARDLYFHAFPLVDITLI
                     PDDEIMQHRSMAAFTLVQKHIRQRDMTTLLDKLSRLMILGQMSGQQIRMLINYMALVG
                     EAQDVRTLVHGLAQRVPQQGEELMTLAEELRRDALLNVAKAMLQRGFDLQLIMEMTGL
                     SQEDLYQLQS"
     gene            complement(6221..6841)
                     /gene="rhtB"
                     /locus_tag="ISB30_003661"
     CDS             complement(6221..6841)
                     /gene="rhtB"
                     /locus_tag="ISB30_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAP1991253.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6952..7974
                     /gene="pldB"
                     /locus_tag="ISB30_003662"
     CDS             6952..7974
                     /gene="pldB"
                     /locus_tag="ISB30_003662"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAP1991254.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHSGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7982..8782
                     /gene="yigL"
                     /locus_tag="ISB30_003663"
     CDS             7982..8782
                     /gene="yigL"
                     /locus_tag="ISB30_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAP1991255.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            8858..9757
                     /gene="bioP"
                     /locus_tag="ISB30_003664"
     CDS             8858..9757
                     /gene="bioP"
                     /locus_tag="ISB30_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAP1991256.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(9645..10598)
                     /gene="metR"
                     /locus_tag="ISB30_003665"
     CDS             complement(9645..10598)
                     /gene="metR"
                     /locus_tag="ISB30_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAP1991257.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10716..12977
                     /gene="metE"
                     /locus_tag="ISB30_003666"
     CDS             10716..12977
                     /gene="metE"
                     /locus_tag="ISB30_003666"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP1991258.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPQRHQNNDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NIDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
BASE COUNT         3122 a         3276 c         3494 g         3191 t
ORIGIN      
        1 ttgtgtaaga atttcgttag catggcaata ttcccgctct tatgcaagag caggcatatt
       61 ttttcagaat atatttattt tttatttgga aattctacgc tgcgtataaa tcgcatccat
      121 cacaaaaatt gccagcgcca cccaaataaa ggcgaaagtc accatcttat cggcacccgg
      181 tttttcacca taaaacgtca cagccagcag gaacatcagc gtcggaccaa tgtactggaa
      241 aaagcctaac gttgagagac gcaggcgtgt cgcagctgca gtaaaacaca acagtggcac
      301 ggtggtgaca ataccggcgg cgatcagcag taaattcagc gacatcgggt tttgccccat
      361 atgactggtt gagctgtcgg caatagcaaa caggtaaatc gccgccacgg gcagcaacca
      421 catagtttcg attaacatgc cggtttgcgc ttcaacagca atcttcttgc gcaccaggcc
      481 gtagaaagca aaactaaacg ccagccccag cgcgatgatc ggcagtgaac caaaagtcca
      541 caactgaacc aacacgccac aaatcgccag aatcaccgcc agccattgca tccggcggaa
      601 tcgctcgccg aggaaaatca tccccagcac aatgttcacc agtgggttaa taaagtaacc
      661 aaggctcgct tccagcatat ggtgattgtt caccgcccag ataaacagta gccagttgcc
      721 accaatcagc acggcagaga ctgccagcat aaaaattttc tgtggcgtct gaatcagcgt
      781 ttttaaatag gaccactggc ggcaaatgct catcagcacc accataaaga aaaacgacca
      841 gatcacgcga tgcgtcagga tctcatcggc gggcacgtag taaatcagtt tgaagtacgc
      901 tggcgcaata ccccaaataa aataagcggc aagagcgagt aatacgccct gccgcgtttg
      961 ttttgcatcc atcgggaata ctcattttta attggtaaca gcagtttacc tgcttttatg
     1021 tcttcaacct accatatagg tggcggtggc actggcaata taaagctgtt cttcattgtg
     1081 taattcaacg cgggcgacgg cgactttatt gcctgcacgc aacaggctac tggtagcagt
     1141 aaaacgctct cctctgcctg ggcgcagata atcgacgcga agatcaatgg tccccatccg
     1201 cgatagccgc tggcgtagtt catcttcact gatggtttcg tggcgggtta aggtacttcc
     1261 cacgcacacc agacctgcgg cgacatccag cgccgacgca atgaccccgc cgtgtaaaat
     1321 gctttgcgcc cagttgccca ccatcatcgg ctgattttta aaggccagct gtgcgaactc
     1381 tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta aatggcatgt gataaacaaa
     1441 catctcaccc actaatttga gggcttgttc agcggtcagt acggcagaca taacatcctt
     1501 acacttcatt agttaatgaa atgttgattt tatgcttatt tattgttggt ttctacttta
     1561 ggaagggata actaacggct atggagttaa gtatgtaaaa tagcccgcag aaaaatattc
     1621 accttatcaa taattcgtta cggagaacac gagcgatgcg gactctgcag ggctggttgt
     1681 tgccggtgtt tatgttgcct atggcagtat atgcacaaga ggcaacggtg aaagaggtgc
     1741 atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat gctgcaggag catgacaatc
     1801 cgttcaccct ttatccttat gacaccaact acatcatcta cacccaaacc agcgatctga
     1861 ataaagaagc gattgccagt tacgactggg cggaaaacgc gcgtaaggat gaagtaaagt
     1921 tccagttgag cctggcgttt ccgctgtggc gtgggatttt ggggccaaac tctgtactgg
     1981 gcgcctccta cacacagaaa tcctggtggc agctttctaa tagcgatgag tcatcaccgt
     2041 ttcgtgaaac taactacgaa ccgcaattgt tcctcggttt tgccacggat tacaactttg
     2101 ctggctggac gctgcgcgat gtggaaatgg gctacaacca cgactcaaac gggcgttcag
     2161 acccgacttc ccgcagctgg aaccgccttt atacccgcct gatggcagaa aacggtaact
     2221 ggttggtaga ggtgaagccg tggtatgtgg tggggaatac ggacgataac ccggacatca
     2281 ccaaatatat gggttactac cagcttaaaa tcggctatca cctcggtgat gcggtactga
     2341 gcgcgaaagg gcagtacaac tggaacaccg gttacggcgg cgcggagtta ggcttaagct
     2401 acccgatcac caaacatgtg cgcctttata ctcaggttta cagcggttac ggcgaatcgc
     2461 tcatcgacta taacttcaac cagacccgcg ttggtgtggg agttatgcta aacgacctgt
     2521 tttgatgaat ggttggcaaa tctggaatcc agcatccagg attaccctct cagagactaa
     2581 aagcattgca gtttctcgcg caggcgctga aaatagcgcc tgtttttatt tcaggcaatc
     2641 ggggtgaatg tggcgcaggc ggaagtgttg aatctggagt ccggagctaa acaggtttta
     2701 caagaaacct ttggctacca acagtttcgc cccggccagg aagagattat cgacaccgta
     2761 ctttccggtc gcgattgcct ggtcgtcatg cccactggtg gcggaaaatc cctttgctat
     2821 caaatccctg ccttattgct aaacggcctt accgtggttg tctcaccgct gatttcgttg
     2881 atgaaagatc aggtagatca actgcaagcc aacggcgtgg cggcggcgtg ccttaactcg
     2941 acgcaaaccc gcgagcagca acttgaagtg atgacaggct gtcgcaccgg gcaaatcagc
     3001 ctgctttata tcgcgccgga acgcctgatg ctggataact ttcttgagca tctggcgcac
     3061 tggaatccgg tgttattagc cgttgatgag gcgcactgta tctcccaatg gggtcacgat
     3121 ttccgcccgg aatacgctgc cctcggtcag ctgcgtcagc gttttccgac gctgccgttt
     3181 atggcgctga ctgccacagc cgatgacacc acgcgccagg atatcgtgcg cctgctgggg
     3241 ctgaacgatc cgctgattca aatcagcagt tttgaccgtc cgaatattcg ctacatgctg
     3301 atggagaaat tcaaaccgct cgatcagttg atgcgttatg tacaggaaca acgcggtaag
     3361 tcggggatta tttactgtaa tagtcgtgcg aaagtagaag acaccgctgc gcgcctgcaa
     3421 agcaagggaa ttagcgcggc ggcctatcat gccgggctgg aaaataatgt tcgcgctgat
     3481 gtgcaggaaa aattccagcg cgatgacctg caaattgtgg tggcgacggt ggcgttcggc
     3541 atgggcatca acaagcctaa cgttcgcttc gtggtgcatt ttgatattcc gcgcaatatc
     3601 gaatcctatt atcaggaaac tggacgcgcc gggcgtgatg gcctgcccgc ggaagcgatg
     3661 ctgttttacg atccggctga tatggcgtgg ctgcgccgtt gtctggaaga gaagccgcag
     3721 gggcagttgc aggatatcga acgtcataaa ctcaatgcga tgggcgcgtt tgctgaagcg
     3781 caaacttgcc gtcgtttggt attgctgaac tattttggcg aagggcgtca ggagccgtgc
     3841 gggaactgcg atatctgcct cgatccgcca aaacagtacg atggttcaac cgatgcgcag
     3901 attgcccttt ccaccattgg tcgtgtgaat cagcggtttg ggatgggtta tgtggtggaa
     3961 gtgattcgcg gtgctaataa tcagcgtatc cgcgactatg gtcatgacaa actgaaagtc
     4021 tatggcatgg gccgtgataa aagccatgaa cattgggtga gcgtgatccg ccagctgatt
     4081 cacctcggcc tggtgacgca aaatattgcc cagcattctg ccctacaact gacagaggct
     4141 gcgcgtccgg tgctgcgcgg cgaatcctct ttgcaacttg ccgtgccgcg tatcgtggcg
     4201 ctcaaaccga aagcgatgca gaaatcgttc ggcggcaact atgatcgcaa actgttcgcc
     4261 aaattacgca aactgcgtaa atcgattgcc gatgaaagca atgtcccgcc gtacgtggtg
     4321 tttaacgacg ctaccttgat tgagatggct gaacagatgc cgatcaccgc cagcgaaatg
     4381 ctcagcgtta acggcgttgg tatgcgcaag ctggaacgct ttggcaaacc gtttatggcg
     4441 ctgattcgtg cgcatgtcga tggtgacgac gaagagtagt cagcagcata aaaaagtgcc
     4501 aggatgaaga ctccgtaaac gtttcccccc gcgagtctaa tgtatgttga tgttattcct
     4561 caccgtcgcc atggtgcaca ttgtggcgct tatgagcccc ggtcccgatt tcttttttgt
     4621 atctcagacc gctgttagtc gttcccgtaa agaagcgatg atgggcgtgc tgggcattac
     4681 ctgcggcgta atggtttggg ctgggattgc gttgcttggc ctacatttga ttatcgaaaa
     4741 aatggcctgg ctgcatacgc tgattatggt gggcggtggc ctgtatctct gctggatggg
     4801 ttaccagatg ctacgtggtg cactgaaaaa agaggttgtt tctgcccctg cgccacaagt
     4861 cgaactggcg aaaagcgggc gcagttttct taagggctta ctgaccaatc tcgcgaatcc
     4921 taaagcgatt atctactttg gttcggtgtt ctcgctgttt gtcggtgata atgtcggcac
     4981 caccgaacgc tggggcattt tcgcgttgat tattgtcgaa acactggcgt ggtttaccgt
     5041 cgttgccagc ctgtttgctc tgccgcaaat gcgctgtggt tatcaacgtc tggcgaagtg
     5101 gattgatggt tttgccgggg cgttatttgc cggatttggc attcatttga ttatttcgcg
     5161 ttaagcgctt cccccctgat acgctatcag ggggaataac atcaggattg caattgataa
     5221 aggtcttcct gagaaagccc ggtcatttcc ataatcaact gcaaatcaaa gccacgctgt
     5281 agcatggctt ttgcgacatt gagaagggca tcgcgccgaa gttcttccgc taaagtcatc
     5341 agttcctctc cttgttgagg cactcgttgt gccagaccat gtactaatgt tcgtacatcc
     5401 tgtgcttcac ctaccagcgc catgtagttt atcaacatgc gtatttgctg tccgctcatc
     5461 tgccccagaa tcattaagcg ggataactta tctaacagcg ttgtcatatc ccgttgacgg
     5521 atatgcttct gtaccagcgt aaaggcggcc atgctgcggt gttgcatgat ttcgtcatca
     5581 ggaattagcg taatatcaac cagcggaaaa gcatgaaaat agagatcgcg tgcaatgtcc
     5641 ggatccggaa aaccatcgag ccagtttgtt gaccatggat atgggctacg ctttccctgg
     5701 taaaaaagaa tggggatcac cagaggcaga taatcatgtc ccgcttcaag atgccgctgc
     5761 atggtggcaa tagcatagcg aagcatacgg aacgccatat tttcatcagg tgaactttga
     5821 tgctcaatca acacatagat atatccgcac cctttcgtcg tctctactga ataaagtacg
     5881 tcgttgtaat actggcgtag gttttcctct acaaaactgc tggactcgag atggagcgta
     5941 tcaagattgc agagtttaac taaagaaggc ggcagatgaa tttcaataaa gtcacgggcc
     6001 gtttctggtc gggataaaaa agccttaaag attgcgtcat gtggtgtcgt tgcttccgtc
     6061 atttttcatc atccgtgggt gaaatatggc ggcattctgg cactaacaaa gagaagtaaa
     6121 tagtaaataa attaacagat tacgcttatt acgagatgcc tcgagcgcgg atgtaacaat
     6181 ctctcgaggc acattaattt acgtgagctt tattccagtt ttaagcatgc ctcgccgatg
     6241 ctaacagcgc tcccaccagc ataaataacg agccgaaaat cttattcagc gccttcatct
     6301 gctttggtcc tttaatccat agagcaatcc gttgagcaag ggtggcgtaa ccgatcatca
     6361 caataatatc gaccacaata gtggtgacgc cgagcacgat atactgcatc agctgcggct
     6421 gttgcggcat gatgaattgc ggaaatagcg ccgccagaaa cacaatactt ttgggattgg
     6481 tgagattgac aaaaactgcg cgctggaaca aatgtcgacg cgattgagta gaggccagcg
     6541 atttaagatc aattgcgcca gcggcgcgcc actgctggat cccaagccaa atcaagtaag
     6601 ctgcgcctgc ccacttcaac acttcaaacg caatcactga gcgggaaaat agcgtcccca
     6661 accccacgcc aaccagcaca atatgaatcg ccagtccggt ctgaagccca gcaatagacg
     6721 ccaccgcgcc gcgataaccg tggttgagcg aggtggtcat ggtgttgatt gcgccagagc
     6781 ctggcgacag gcttaaaatg atcgatgtca gcaggtaggc aaaccaccat tctaaggtca
     6841 tgatgaactc ccggtgtgtc tatttttgtg ccacaatacg ctactgtcgc agcgttgtgt
     6901 caggcacgct taaaaaaacg attttacgtg gtttaagagg cagattaccc gatgtttcag
     6961 cagcaaaaag actgggaaac aagagaaaac gcgtttgctg cttttaccat gggaccgctg
     7021 actgatttct ggcgtcagcg tgatgaagca gagtttactg gtgtggatga cattccggtt
     7081 cgctttgtcc gttttcgcgc acagcaccat gaccgggtgg tagttatctg cccggggcgt
     7141 attgagagct acgtaaaata tgcggaactg gcctacgacc tgttccattc ggggtttgat
     7201 gttttaatca tcgaccatcg cgggcaggga cgttccggtc gcctgttagc cgatccacat
     7261 ctcgggcatg ttaatcgctt taatgattat gttgatgatc tggcggcatt ctggcaacag
     7321 gaggttcagc ccggtccgtg gcgtaaacgc tatatactgg cacattcgat gggcggtgcg
     7381 atctccacat tatttctgca acgccatcca ggtgtatgtg acgccattgc gctaactgcg
     7441 ccaatgtttg ggatcgtgat tcgtatgccg tcatttatgg cacggcagat cctcaattgg
     7501 gccgaagcgc atccacgttt ccgtgatggc tatgcaatag gcaccgggcg ctggcgcgcg
     7561 ttgccgtttg ctatcaacgt actgacccac agcagacagc gatatcgacg taacttacgt
     7621 ttctatgctg atgacccaac gattcgcgtc ggtgggccga cctaccattg ggtacgcgaa
     7681 agtattctgg ctggcgaaca ggtgttagcc ggtgcgggtg atgacgccac gccaacgctt
     7741 ctcttacaag ctgaagagga acgcgtggtg gataaccgca tgcacgaccg tttttgtgaa
     7801 ctacgcaccg ctgcgggcca tcctgtcgaa ggaggacggc cgttggtaat taaaggtgcg
     7861 taccatgaga tcctttttga aaaggacgca atgcgctcag tcgcgctcca cgctatcgtt
     7921 gattttttca acaggcataa ctcacccagc ggaaaccgct ctacagaggt ttaaatttct
     7981 tatgtaccag gttgttgcgt ctgatttaga tggcacgtta ctttctcccg accatacgtt
     8041 atccccttac gccaaagaaa ctctgaagct gctcaccgcg cgcggcatca actttgtgtt
     8101 tgcgaccggt cgtcaccacg ttgatgtggg gcaaattcgc gataatctgg agattaagtc
     8161 ttacatgatt acctccaatg gcgcgcgcgt tcacgatctg gatggcaacc tgatttttgc
     8221 tcataacctg gatcgcgaca ttgccagcga tctgtttggc gtagtcaacg acaatccgga
     8281 catcattact aacgtttatc gcgacgacga atggtttatg aatcgccatc gcccggaaga
     8341 gatgcgcttt ttcaaagaag cggtgttcaa ttatgcgctg tatgagccgg gattgctgga
     8401 gccggaaggc gtcagcaaag tgttcttcac ctgcgattcc catgaaaaac ttctgccact
     8461 ggagcaagcg attaacgctc gctggggcga tcgcgtcaat gtcagtttct ctaccttaac
     8521 ctgcctggaa gtcatggctg gcggcgtttc aaaaggccat gccctggaag cggtagcgaa
     8581 gaaactgggt tacagcctgc aagattgtat tgcctttggc gatggaatga acgatgccga
     8641 aatgctgtcg atggcgggga aaggctgcat tatgggcagc gcgcatcaac ggctgaaaga
     8701 tctgcatcca gagctggaag tgattggtac taacgccgaa gacgcagtgc cgcattatct
     8761 gcgtaagctc tatttatcgt aatcgttctt tatttggtca gttgtcaacc tgatacttcg
     8821 ctacaatgga tactcgttaa tcaaagagtt ttccattgtg gcgctactta tcatcaccac
     8881 gattctgtgg gccttctcct ttagctttta tggcgagtac cttgcggggc acgtcgatag
     8941 ctattttgcg gtgctggtgc gcgttggtct ggcggcactt gtttttctgc cgtttctgcg
     9001 tacccgtggc aatagcctga aaacggtcgg cctgtatatg ctggtgggcg cgatgcagct
     9061 cggtgtgatg tatatgctga gtttccgcgc ctatctctac ctgacggttt ctgaactgct
     9121 gttgttcacc gtactgacgc cgctctacat cacgctgatt tatgacatca tgagtaagcg
     9181 ccgtctgcgc tggggctatg cttttagcgc cttgttggcg gtgattggtg ccgggattat
     9241 tcgctatgat caggtcaccg accatttctg gactggcttg ttgctggtgc aactctccaa
     9301 tatcactttt gccatcggca tggtgggtta caaacgtctg atggaaactc gcccgatgcc
     9361 gcagcataac gcctttgcgt ggttctatct tggcgcgttc ctggtggcag tgattgcatg
     9421 gttcttgctg ggaaatgcgc agaaaatgcc gcaaaccacg ttgcaatggg gcattctggt
     9481 gtttcttggc gtggtggctt ccgggattgg ctactttatg tggaactacg gcgcgacgca
     9541 ggtggacgcc ggaacgctgg gcattatgaa taatatgcac gttccggcag ggctgctggt
     9601 aaacctggct atctggcacc aacagccgca ctggccaacg tttattacag gcgcgctggt
     9661 gatcctggcc tcactgtggg tgcatcgtaa gtgggtcgct ccgcgctctt cacaaacggc
     9721 agatgatcgc aggcgtgatt gcgcgctgaa cgaataaacg cttctgtaat cggctgacgc
     9781 tgctcgccat cgcgcacggc ggcgtacagt cggctccaca agccttcgcc cagggttttg
     9841 gtgaccacca gaccctggcg ttcaaaactc tccaccaccc aatgaggtag tgcggcaata
     9901 cccatccgtg cggcaaccat ctgaatcaac aataaggtgt tatcgacgct tttcagtgac
     9961 gggctgacgc ctgctggctg aagaaaatgc cgccagacat ccagtcggct acgctgcacc
    10021 ggataaatca acagcgtttc gctggcgaga tcttccggtg taatacgcgt ttttgccgcc
    10081 agtggatggt caggtgctaa caccagacgt acttcatagt cgaacatcgg cgaataatgc
    10141 aggccactgc gcggcagaat atcggacgtc atcaccagat ccagctcgcc ctgttgcaac
    10201 gcaggctgcg gatcaaacgt tacgcccgat ttaaaatcca tctccacctg cggccagttc
    10261 ttatggaaat tttctaatgc gggcgtcagc cactgaatac agctatggca ctctatggca
    10321 atgcgcaggc gcgtctgctg cggttcattg caggcttgca gtgcctggct aatttgcggc
    10381 agcacctgat ttgccagttg caacaggatt tctccctgcg gtgtaaagcg aagcggctgg
    10441 cttttacgca caaatagccg gaagccaagg cgttgttcca gatcgctaaa ctggtgagac
    10501 agggcggatt gcgtttgatg caacgtcgcc gcagcggctg cgagtgagcc gcagttccgc
    10561 aacgcttgta gcgttttcag gtgttttact tcgatcatga aagtccttca cttcggcatg
    10621 aataatttgc gcttgaggaa tatacagtaa ccgccaatta tggatgtgta aacatctgga
    10681 cggctaaata ctaaaaattc cataaggggc atataatgac aattcttaat cacaccctcg
    10741 gtttccctcg cgttggcctg cgtcgcgagc tgaaaaaagc gcaagagagt tattgggcgg
    10801 ggaactccac gcgtgaagaa ctgctggcgg tagggcgtga attgcgtgct cgtcactggg
    10861 atcaacaaaa gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc
    10921 atgtactgac aaccagtctg ctgctgggta atgttccgca acgtcatcag aacaacgatg
    10981 gttcggtaga tatcgatacc ctgttccgta ttggtcgtgg acgtgcaccg actggcgaac
    11041 ctgcggcggc agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg
    11101 agttcgttaa aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg
    11161 aggcgctggc gctgggccac aaggtgaaac ctgtgctgct ggggccggtt acctacctgt
    11221 ggctgggtaa agtgaaaggt gaacagtttg atcgcctgag cctgctgaac gacattctgc
    11281 cggtttatca gcaagtgctg gcagaactag cgaaacgcgg cattgagtgg gtacagatcg
    11341 acgaacccgc gctagtgctg gaactgccgc aggcgtggct ggatgcatac aaacccgctt
    11401 acgacgcgct ccagggccag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc
    11461 caaacctcga cacgattact gcgctgcctg ttcagggtct gcatgtcgat cttgtacatg
    11521 gtaaagatga cgttgctgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg
    11581 gtcttatcaa tggtcgtaac gtctggcgcg ccgatcttac cgagaaatat gcgcaaatta
    11641 aggacattgt cggcaaacgt gatttgtggg tggcatcttc ctgctcactg ctgcacagcc
    11701 ccatcgacct gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg
    11761 ccctgcaaaa atgtcatgaa ctggcattgc tgcgcgatgc gctgaacagt ggtgatacgg
    11821 cagctctggc agagtggagc gctccgattc aggcgcgtcg tcactctact cgtgtacata
    11881 atccggcagt agaaaagcgt ctggcggcga tcaccgccca ggacagtcag cgtgcgaatg
    11941 tctatgaagt gcgtgctgaa gcccagcgtg cgcgttttaa actgcccgcg tggccgacca
    12001 ccacgattgg ttccttcccg caaaccacgg agattcgtac cctgcgtctg gattttaaaa
    12061 agggtaatat cgacgccaat aactaccgca cgggcattgc ggaacatatc aagcaggcca
    12121 ttgttgagca ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg
    12181 acatggtgga atactttggc gagcatctgg atggctttgt ctttacgcaa aacggttggg
    12241 tacagagcta cggttcccgc tgcgtgaagc caccgattgt tattggtgac gttagccgcc
    12301 cggcaccgat taccgtggag tgggcaaaat atgcgcaatc cctgactgat aaaccggtga
    12361 aagggatgtt gactggcccg gtgactattc tctgctggtc gttcccgcgt gaagatgtca
    12421 gccgtgaaac catcgccaaa cagattgcgc tggcactgcg tgatgaagtg gccgatctgg
    12481 aagccgctgg aattggcatc attcagattg acgaaccggc attgcgcgaa ggtttaccac
    12541 tgcgtcgcag cgactgggat gcctatctcc agtggggcgt ggaggctttc cgtatcaacg
    12601 ccgccgtggc gaaagatgac acacaaatcc acactcacat gtgttactgc gagttcaacg
    12661 acattatgga ttcgattgcg gcgttggacg cagacgtcat caccatcgaa acctcgcgtt
    12721 ccgacatgga gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg
    12781 gcgtctatga cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga
    12841 agaaagcggc aaaacgcatt ccggcagagc gtctgtgggt caacccggac tgtggcctga
    12901 aaacgcgcgg ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga
    12961 atttgcgtcg ggggtaaaat ccaaaccggg tggtaatacc acccggtctt ttctcattac
    13021 agcgacgtct tcccagcata ctgcgtaaac cattccagat atcagttcat accctgttta
    13081 aga
//
LOCUS       DACADF010000130        13079 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.132, whole genome shotgun sequence.
ACCESSION   DACADF010000130 DACADF010000000
VERSION     DACADF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.132"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            190..525
                     /locus_tag="ISB30_003667"
     CDS             190..525
                     /locus_tag="ISB30_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAP1991259.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            685..1128
                     /gene="yjdN"
                     /locus_tag="ISB30_003668"
     CDS             685..1128
                     /gene="yjdN"
                     /locus_tag="ISB30_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAP1991260.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSTQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVIKQQPTQ"
     gene            1261..2049
                     /gene="phnC"
                     /locus_tag="ISB30_003669"
     CDS             1261..2049
                     /gene="phnC"
                     /locus_tag="ISB30_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAP1991261.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2074..3090
                     /gene="phnD"
                     /locus_tag="ISB30_003670"
     CDS             2074..3090
                     /gene="phnD"
                     /locus_tag="ISB30_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1991262.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3195..3974
                     /gene="phnE"
                     /locus_tag="ISB30_003671"
     CDS             3195..3974
                     /gene="phnE"
                     /locus_tag="ISB30_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAP1991263.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3995..4720
                     /gene="phnF"
                     /locus_tag="ISB30_003672"
     CDS             3995..4720
                     /gene="phnF"
                     /locus_tag="ISB30_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAP1991264.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4721..5173
                     /gene="phnG"
                     /locus_tag="ISB30_003673"
     CDS             4721..5173
                     /gene="phnG"
                     /locus_tag="ISB30_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAP1991265.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5170..5754
                     /gene="phnH"
                     /locus_tag="ISB30_003674"
     CDS             5170..5754
                     /gene="phnH"
                     /locus_tag="ISB30_003674"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAP1991266.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNASLVNQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5754..6818
                     /gene="phnI"
                     /locus_tag="ISB30_003675"
     CDS             5754..6818
                     /gene="phnI"
                     /locus_tag="ISB30_003675"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAP1991267.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGGQLLGPTYDYTHRLLDFTLLANGETPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDIFIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6811..7656
                     /gene="phnJ"
                     /locus_tag="ISB30_003676"
     CDS             6811..7656
                     /gene="phnJ"
                     /locus_tag="ISB30_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAP1991268.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7653..8411
                     /gene="phnK"
                     /locus_tag="ISB30_003677"
     CDS             7653..8411
                     /gene="phnK"
                     /locus_tag="ISB30_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAP1991269.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVMELNLAVVIVTHDLGVARLLADRLLV
                     MKQGKVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8522..9202
                     /gene="phnL"
                     /locus_tag="ISB30_003678"
     CDS             8522..9202
                     /gene="phnL"
                     /locus_tag="ISB30_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAP1991270.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            9199..10335
                     /gene="phnM"
                     /locus_tag="ISB30_003679"
     CDS             9199..10335
                     /gene="phnM"
                     /locus_tag="ISB30_003679"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAP1991271.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGKIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVEREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARHIALASHDDATHAHVAQSHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10335..10892
                     /gene="phnN"
                     /locus_tag="ISB30_003680"
     CDS             10335..10892
                     /gene="phnN"
                     /locus_tag="ISB30_003680"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAP1991272.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRQREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            10879..11313
                     /gene="phnO"
                     /locus_tag="ISB30_003681"
     CDS             10879..11313
                     /gene="phnO"
                     /locus_tag="ISB30_003681"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAP1991273.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11315..12073
                     /gene="phnP"
                     /locus_tag="ISB30_003682"
     CDS             11315..12073
                     /gene="phnP"
                     /locus_tag="ISB30_003682"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAP1991274.1"
                     /translation="MSLILTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVE"
     gene            complement(12101..12430)
                     /gene="yjdP"
                     /locus_tag="ISB30_003683"
     CDS             complement(12101..12430)
                     /gene="yjdP"
                     /locus_tag="ISB30_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HAP1991275.1"
                     /translation="MKRFPLFLLFTLFTLFTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDERQRQLDQERQQLEDEERR
                     MEDEYGR"
     gene            complement(12499..12948)
                     /gene="rpiB"
                     /locus_tag="ISB30_003684"
     CDS             complement(12499..12948)
                     /gene="rpiB"
                     /locus_tag="ISB30_003684"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HAP1991276.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
BASE COUNT         2929 a         3900 c         3606 g         2644 t
ORIGIN      
        1 ttatcttaaa taataaatgg aggcaaaaag attattttct ttttgcgttt cctttcaaat
       61 gaaaacgatc gtcatctaaa atcagcagta cccccgacaa actcagggat tttgtgtata
      121 attgcggcct ttttcggcaa tctgccgttt tttggcgctt ttgccctgct gacttttgag
      181 gaaatccaca tgtcattacc acactgccca aaatgcaact ccgaatacac ctacgaagat
      241 aacggcatgt acatctgccc ggaatgtgcc tacgaatgga acgacgcaga acctgcacag
      301 gaaagcgacg agctgatcgt taaagatgct aacggcaatc tgctggctga cggcgacagc
      361 gttaccatca ttaaagatct gaaagtgaaa ggtagctctt cgatgctgaa aattggcacc
      421 aaagtaaaaa acatccgcct ggttgaaggc gaccataaca tcgattgcaa aatcgacggt
      481 tttggtccga tgaaactgaa gtctgagttt gtgaaaaaga actgattgta ttgtgatcgg
      541 taggccggat aaggcgctcg cgccgcatcc ggcaacggtg gcgactgcct gatgcgacgc
      601 tgaccgcgtc ttatcaggct tccctctcat ctgtataaat ttcgaactac acttaactgg
      661 cttctcttaa ctgaggtcac catcatgccg ttaagtccct acctctcttt tgccggtaac
      721 tgttccgacg cgattgccta ttatcaacgt acgttgggcg cggaactgct ctataaaatc
      781 agcttcggcg aaatgccaaa atcaacgcag gacagcgccg agaactgccc ttccggaatg
      841 caatttcccg ataccgccat cgcccacgcc aatgtgcgca ttgcaggaag cgacatcatg
      901 atgagcgatg ctataccgtc aggaaaagcc agctactccg gctttacgct ggtgctcgat
      961 tctcaacagg tggaagaagg aaaacgctgg tttgataacc ttgccgccaa cggaaaaatc
     1021 gaaatggcct ggcaggaaac gttctgggcg catggctttg gcaaagtcac cgataaattt
     1081 ggcgtaccgt ggatgattaa tgtcatcaaa caacagccaa cgcaataacc cgccgggagg
     1141 cccgccctcc cgcactgtca tcgaattccc gttaactctt catctgttag tcacttttaa
     1201 ttaaccaaat cgtcacaata atccgccacg atggagccac ttttttaggg aggctgcatc
     1261 atgcaaacga ttatccgtgt cgagaagctc gccaaaacct tcaatcagca tcaggcgctg
     1321 cacgcggttg atctgaacat tcatcatggt gaaatggtgg ctctgcttgg gccgtcgggt
     1381 tccggaaaat ccaccctttt acgtcactta agcggtttga ttaccggcga taaatctgtc
     1441 ggtagccata tcgagctgct gggccgcaca gtccagcgcg aaggccgcct ggcccgcgat
     1501 atccgcaaaa gccgcgccaa caccggctac atctttcaac aattcaacct ggtgaatcgc
     1561 ctgagcgtac tggagaacgt gctgattggc gcgctcggca gcacgccatt ctggcgaacc
     1621 tgttttagct acttcacccg cgagcagaaa cagcgcgcgt tacaggcgct gacccgcgtt
     1681 ggcatggtgc actttgccca tcaacgcgtt tccacactct ccggcggcca gcaacagcgt
     1741 gtggcgattg cccgcgcgct gatgcagcag gcgaaggtca ttctcgctga cgaacccatc
     1801 gcctcgctgg acccggaatc ggcacgcatc gtgatggaca ccctgcgcga catcaaccag
     1861 aacgacggca ttaccgtggt cgtcacgctg catcaggtgg attacgccct gcgctactgc
     1921 gaacgcatcg tcgccctgcg ccaggggcac gtcttctacg acggctgcag ccaacagttt
     1981 gataacgaac gttttgacca tctctaccgc agcattaacc gcgtcgaaga gaacgcgaaa
     2041 gctgcctgac atccccatca ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg
     2101 gccttcacca gcatgttcag cctcagcacc ctgttaaatc cggcatacgc cgaagagcag
     2161 gaaaaggcgc tgaatttcgg cattatttca acggaatcac agcaaaacct gaaaccgcaa
     2221 tggacgccgt tcttacagga tatggagaag aagctgggcg taaaggtcaa cgccttcttt
     2281 gccccggact acgcgggcat tatccagggg atgcgcttca ataaagtgga tatcgcctgg
     2341 tacggcaacc tgtcggcgat ggaagcggtg gatcgcgcca acggtcaggt cttcgcccag
     2401 acggtcgcgg cggatggatc gccgggttac tggagcgtgt tgatcgtcaa caaagatagc
     2461 ccgatcaaca acctgaacga tctgctggcg aagcggaaag atctcacctt cggcaatggc
     2521 gatcctaact ctacctccgg cttcctcgtc cccggttact acgtcttcgc taaaaacaat
     2581 atctccgcca gcgactttaa gcgcaccgtc aacgccgggc atgaaaccaa cgccctcgcc
     2641 gtcgctaaca agcaagtgga tgtcgccacc aacaacaccg aaaacctcga caagctgaaa
     2701 acctccgcgc cggagaagct gaaagaactg aaggtgatct ggaaatcacc gctgatccct
     2761 ggcgatccga tcgtctggcg caaaaatctt tccgaaacca ctaaagacaa gatctacgac
     2821 ttctttatga attacggcaa aacgccggaa gagaaagcgg tgctggagcg cctgggctgg
     2881 gcgccgttcc gcgcctccag cgacttgcaa ctggtgccga ttcgccagct cgcactgttt
     2941 aaagagatgc aaagcgtgaa ggacaacaaa gggctgaacg aacaggacaa gctggcaaaa
     3001 accaccgcca ttcaggcgca actggatgat ttggaccgcc tgaacaacgc gttaagcgca
     3061 atgagttcgg tgagtaaagc ggtgcagtaa atcgtaggtc ggataagacg cccggcgtcg
     3121 catccgacaa tgtgcagacg ttgatgccgg atgcggtgca agcaccttat ccggcctaca
     3181 gaccggagcc aaacatgcaa accatcacca tcgccccacc caagcgcagc tggttctcgc
     3241 ttctgagctg ggccgttgtt atcgccgtgc tggtcgtctc gtggcagggc gcggaaatgg
     3301 ccccgcttac gctgattaaa gatggcggca acatggcgac gttcgccgcc gacttcttcc
     3361 cgcccgattt cagccagtgg caggattacc tcaccgaaat ggccgtcacg ctgcaaatcg
     3421 ccgtctgggg caccgcgctg gcggtggttc tctccatccc cttcggcctg atgagcgctg
     3481 aaaacctggt accgtggtgg gtctaccaac ctgtacgccg cctgatggac gcctgccgcg
     3541 ccattaacga aatggtcttc gccatgctgt tcgtggtcgc cgtcggcctc ggcccgttcg
     3601 ctggcgtgct ggcgctgttt atccacacca ccggcgtgct ctccaagctg ctttccgaag
     3661 cggtggaagc gatcgaaccc ggcccggtgg agggcattcg cgccaccggt gccaacaagc
     3721 tggaagagat cctttacggc gtactaccgc aggtcatgcc gctgctgatt tcctactctc
     3781 tctatcgctt cgaatccaac gtccgctcgg cgaccgtcgt cggtatggtt ggcgcgggcg
     3841 ggatcggcgt cacgctgtgg gaagcgattc gcggtttcca gttccagcaa acctgcgccc
     3901 tgatggtgct catcatcgtc acggtcagcc tgctggattt cctctctcaa cggttgcgta
     3961 agcactttat ctgataagcg aggcattgat atctatgcac ttgtctacac atccgaccag
     4021 ctacccaaca cgctatcagg agatagccgc aaaacttgag caggagcttc gtcaacacta
     4081 ccgctgcggc gactatcttc ccgccgagca gcaactggcg gcgcgctttg aggtgaaccg
     4141 ccacaccctg cgccgcgcca tcgaccaact ggtggaaaaa ggctgggtac agcgccgtca
     4201 gggcgtcggc gtactggtgc tgatgcgccc gttcgattac ccgctcaacg cccaggcgcg
     4261 ttttagccag aacctgctgg accagggcag ccatcccacc agcgaaaaat tgctatcggt
     4321 gctgcgcccc gcctccggtc acgtcgctga cgcgctgggg ataaccgagg gggagaacgt
     4381 catccacctg cgcaccctgc gtcgggtcaa cggcgtcgcg ctctgtttaa tcgaccacta
     4441 ctttgcggac ctcaccctct ggccgacgct gcaacgtttc gacagcggtt cgctgcacga
     4501 ttttctgcgc gagcaaacgg ggattacgct acgacgcagt cagacgcgga tcagcgcccg
     4561 tcgcgcccag gccaaagagt gccagcgtct tgaaatcccg aatctgtcgc cgctgctgtg
     4621 cgtgcgcacc cttaaccacc gtgacggtga aagcagcccg gcggagtact ccgtcagcct
     4681 gacgcgcgcc gacatgattg aattcactat ggagcactga atgcacgcag ataccgcgac
     4741 ccgccagcac tggatgtccg tgctggcgca cagccaaccg gctgaactgg cagcccgcct
     4801 gaacgcgctg aacatcaacg ccgattatga ggtgatccgc gccgctgaaa ctggcctggt
     4861 gcagattcag gcgcgaatgg gcggcaccgg cgaacgcttt tttgctggcg acgccacgct
     4921 cacccgcgcc gccgtgcgtc tgactgacgg cacgctcggc tatagctggg tgctggggcg
     4981 cgacaaacag cacgccgaac gttgtgcgct gattgatgcg ctgatgcagc aaaaccgcca
     5041 ctttcaaaac ttatcagaaa cccttatcgc cccgctggac gctgaccgta tggcacgcat
     5101 tgccgcgcgc caggccgaag tgaacgccag ccgggtcgac ttctttacga tggttcgcgg
     5161 agacaacgca tgaccctgga aaccgctttt atgcttcccg tgcaggatgc ccagcacagt
     5221 tttcgtcgcc tgttaaaggc catgagcgag ccgggcgtga ttgtcgccct gcatcagctc
     5281 aagcgcggct ggcagccgct gaatatcgcc accaccagcg tgctgctgac gctggccgat
     5341 aacgacacgc cggtgtggct tgccgcgcca ttgagcaacg atatcgtcaa tcagagcctg
     5401 cgttttcata ccaacgcgtc gctggttaat cagccggaac aggcgacctt cgcggtaaca
     5461 gatgaggcga tctccagcga gcagctcaac gccctttcca ccggcactgc cgttgcacca
     5521 gaagcgggcg caacgctgat tttacaggtc accagcctga gcggcgggcg tatgttgcgc
     5581 ctcaccggcg caggtattgc cgaagaacgg atgatcgccc cgcaactgcc aaagtgcatt
     5641 cttcatgaac ttaccgagcg cccgcacccg ttcccgctcg gcatcgacct gatcctgacc
     5701 tgcggcgagc gcctgctggc tattccgcga accactcatg tggaggtgtg ctgatgtacg
     5761 ttgccgtgaa agggggcgaa aaggcgatcg acgccgccca cgccctgcaa gagagccgac
     5821 gccggggcga tactgatttg cctgaactga gcgtcgccca gattgaacag cagcttaacc
     5881 tcgcggtaga tcgcgtgatg accgaaggcg gtattgccga ccgcgagctg gccgcgctgg
     5941 cgctgaaaca ggccagcggc gataacgtcg aagcgatttt tctgttgcgc gcctaccgca
     6001 ccacgctggc gaagctggcg gtcagtgagc cgctcgacac caccgggatg cgtctcgaac
     6061 ggcgtatttc agcaatttat aaagacattc ccggcggcca actgcttggc cccacctacg
     6121 actacaccca tcgcctgctc gatttcaccc tgctggcaaa cggcgaaacg ccaacgctga
     6181 ccaccgctga cagcgaacag caaccgtcgc cacacgtctt cagcctgctg gcgcgtcagg
     6241 ggctggcgaa gtttgaagaa gatagcggcg cacaaccgga cgacatcacc cgcacgccgc
     6301 cggtttatcc ctgttcgcgt tcttcccgtc tacaacaatt aatgcgcggt gacgaaggct
     6361 atttgttggc gctggcctac tccacccaac gcggttacgg gcgcaatcac ccgttcgcag
     6421 gcgaaattcg cagtggctat atcgatatat ttattgtgcc ggaagagctg ggatttgcgg
     6481 tgaacgtcgg cgaattgttg atgaccgagt gtgaaatggt caacggtttt atcgacccgc
     6541 cggatgaacc accgcatttt acacgcggct atgggctggt gttcggcatg agcgagcgca
     6601 aagcaatggc gatggcgctg gttgaccgtg cgttgcaggc accggaatac ggcgagcacg
     6661 caacaggtcc ggcacaggat gaagagttcg tgctggccca tgccgacaac gtcgaagccg
     6721 caggctttgt ttcgcatctc aaactcccgc actacgtcga tttccaggcc gaactggagc
     6781 tactcaaacg tctgcaacag gagcagaacc atggctaatc tgagcggcta caactttgct
     6841 tacctcgacg agcagaccaa acgcatgatc cgccgcgcta tcttaaaagc ggtggcaatc
     6901 cccggttatc aggtgccgtt tggcggacgc gagatgccga tgccctacgg ctggggaacc
     6961 ggcggcattc agctcaccgc cagcgtgatt ggcgaaagcg acgtgttgaa ggtgattgac
     7021 cagggcgcag acgacaccac caacgccgtg tcgattcgta acttctttaa gcgcgtgacc
     7081 ggggtgaaca ccaccgagcg cacggacgat gcgacactta tccagacgcg tcaccgcata
     7141 cccgaaacgc cgctgaccga agatcagata gttatcttcc aggtgccgat ccccgaaccg
     7201 ctgcgcttta ttgagccgcg tgagacggaa acgcgcacca tgcacgcgct ggaggagtac
     7261 ggcgtgatgc aggtgaagct gtatgaagat atcgcccgct tcggtcatat cgccaccacc
     7321 tacgcctatc cggtgaaggt gaacgggcgc tacgtgatgg acccgtcgcc gatcccgaaa
     7381 ttcgataacc cgaaaatgga catgatgccc gccctgcaac tgtttggcgc ggggcgcgag
     7441 aagcgcatct atgccgtgcc accgtttacc cgcgtggaaa gcctcgattt cgatgatcat
     7501 ccgttcaccg tccagcagtg ggatgaacca tgcgccatct gcggatcgac ccacagctat
     7561 cttgatgaag tggtgctgga tgacgccgga aaccgcatgt ttgtctgctc cgataccgat
     7621 tattgccgcc aacagagcga ggccaaaagc caatgaatca accgttactt tcggtcaata
     7681 acctgaccca cctttatgcc ccgggcaaag gcttcagcga tgtctctttt gatttatggc
     7741 cgggagaagt tctgggcatt gttggggagt ccggttccgg gaagaccacg ctactgaaat
     7801 caatttccgc ccgcctgaca ccacagcaag gggaaattcg ctacgagaac cgttcgctgt
     7861 acgcgatgag cgaggccgac cgccgtcgcc tgctgcgcac cgaatggggt gtggtgcatc
     7921 agcatccgct tgacggcctg cgccgccagg tgtcggcagg cggcaatatc ggcgaacggt
     7981 tgatggcaac cggagcgcgt cattacggcg atattcgcgc caccgcgcag aaatggctgg
     8041 aagaggtgga gattcccgcc aaccggatcg acgacctgcc gaccaccttt tccggcggca
     8101 tgcagcaacg tttgcagatt gcccgcaacc tggtgacgca tccgaagctg gtgtttatgg
     8161 atgaacccac tggcgggctg gatgtgtcgg tacaggcgcg cctgctggac ctgctccgcg
     8221 gtctggtgat ggagcttaac ctggcggtag tgattgtcac ccacgattta ggcgtcgccc
     8281 gtctgctggc ggaccgtttg ctggtaatga agcaagggaa agtggtggag agtgggttaa
     8341 ccgaccgcgt gctcgacgac ccgcaccatc cgtataccca gttgctggtg tcatcggttt
     8401 tgcagaattg agctggtgcc ggatgcggcg taaacgcctt atccggccta caaatgcgct
     8461 ccccgtaggt cggataagac gcatcagcgt cgcatccgac acccgaacca cgaggcgaaa
     8521 aatgattaac gtacaaaacg tcagtaaaac cttcatcctg caccagcaaa acggcgtgcg
     8581 cctgcctgta ttacaaggcg cctcactcac cgtcaacgcc ggcgaatgcg tggtactcca
     8641 cggccattcc ggcagtggaa aatcaactct gctacgctcg ctgtatgcca actatctacc
     8701 cgacgaaggg caaatccaga tcaaacacgg tgatgaatgg gtcgacctgg tcaccgcgcc
     8761 agcgcgcaaa gtggtggaga tccgcaaaac cactgtcggc tgggtaagcc agtttctgcg
     8821 cgtcatccca cgtatttcgg cactggaagt ggtgatgcag ccgctgctcg ataccggcgt
     8881 accgcgtgaa gcctgcgccg ctaaagccgc gcgtctcctc acccgcctga acgtgcctga
     8941 acgcctgtgg caccttgcgc catcgacctt ctccggtggc gaacagcagc gtgtcaacat
     9001 cgcccgtggc tttatcgtcg actaccccat tctgctgctc gacgaaccta ccgcctcgct
     9061 ggacgctaaa aacagcgccg ccgtagtgga actgatccgc gaagccaaag cccgcggcgc
     9121 ggccatcgtc ggtatcttcc atgacgaaac cgtacgcgag caagtcgccg accgcctgca
     9181 cccaatggga gtctcttcat gattatcaat aacgtcaaac tggtgctgga aaacgaggtg
     9241 gtacacggtt ctctggagat gcaggacggc aaaatccgcg cctttgccga aagccagagt
     9301 cacctgtcag aagcaatgga cggcgaaggc ggctggctgc taccggggct gattgagctg
     9361 cataccgata atctggataa attcttcacc ccgcgcccga aagttgactg gcctgcccac
     9421 tcggcgatga gcagccacga cgcgctgatg gtggcgagcg gcatcaccac cgtgctggac
     9481 gccgtggcga ttggtgatgt acgcgacggc ggcgatcggc tggagaatct ggagaagatg
     9541 atcaacgcca tcgaagagac gcagaaacgc ggcgtcaacc gtgccgagca ccgtctgcac
     9601 ctacgctgcg aactgccgca tcacaccacg ctgccgctgt ttgaaaaact ggtcgagcga
     9661 gaacccgtga cgctggtgtc gctgatggat cactcaccgg gccagcgcca gttcgccaac
     9721 cgcgaaaaat atcgtgaata ttatcagggc aaatactcgc tcaccgatgc gcagatgcaa
     9781 cagtacgaag aggagcaact ggcgctcgcc gcacgctggt cacagccgaa tcgcgaatcc
     9841 atcgccgcaa tgtgccgcgc gcgacacatt gcgctcgcca gccacgatga cgccacgcac
     9901 gcccacgtag cccaatccca tcagcttggc agcgtgatcg ccgaatttcc taccacattc
     9961 gaagcggcag aagcctcgcg caagcacggc atgaacgtac tgatgggcgc gccgaatatc
    10021 gtgcgcggcg gttcgcactc cggcaacgtg gcggccagtg aactggcgca gcttggcctg
    10081 ctggatatcc tctcttccga ctactacccc gccagcctgc tggatgcggc gtttcgcgtg
    10141 gccgatgacc agagcaaccg ctttacgcta ccgcaggcgg tgaggctggt gaccaaaaac
    10201 ccggcccagg cactgaatct ccaggatcgc ggggtgattg gcgagggcaa acgcgccgac
    10261 ctggtgctgg cgcatcgcaa gggcaatcac attcatattg accacgtctg gcgtcagggt
    10321 aaaagggtgt tctgatgacg ggaaaactga tttggttaat gggtccgtcc ggctccggga
    10381 aagacagcct gctggcggaa ctccgccagc gggaacaaac ccagctactg gtagcgcatc
    10441 gctacatcac gcgtgatgcc agcgccggaa gtgaaaatca tatcgcctta agcgagcggg
    10501 agttttttac ccgcgcgggg caaaatctgt tggccttgag ctggcatgct aatggtttgt
    10561 attacggtgt cggcattgag atcgacctct ggctgcacgc cggattcgac gtcgtggtta
    10621 acggctcacg cgcccacctg ccgcaggcgc gggcacgtta tcaatcggca ctgctgccca
    10681 tctgtttaca ggtttcgccg gagatcctgc gccagcgcct ggaaaaccgt ggccgcgaaa
    10741 acgccagtga aattaacgcc cggctggcgc gcgccgcccg ctatactcct caggattgtc
    10801 ttacgctcaa taatgacggc agcctgcgcc agtcggtcga caagctgctg acgctgattc
    10861 atcagaagga gaaacaccat gcctgcttgt gagcttcgcc cggctacgca gtacgacacc
    10921 gacgcagttt acgcgctgat ttgtgagcta aaacaggcgg agtttgacca ccacgcgttt
    10981 cgcgtggggt ttaacgccaa tctgcgcgat ccgaacatgc gctaccatct ggcgctgctg
    11041 gatggcgaag ttgtcggcat gatcggcctg catttgcagt ttcatctgca tcatgtcaac
    11101 tggatcggcg aaattcagga gttggtggtg atgccgcagg cgcgcggcct gaacgtcggt
    11161 agcaagttgc tggcgtgggc agaagaagaa gcccgccagg ccggggccga aatgaccgag
    11221 ctttcgacca acgtgaagcg ccacgacgcg caccgtttct atctgcgcga aggctacgag
    11281 cagagccatt tccgcttcac caaagcgctg taacatgagc ctgatcctca cgctcaccgg
    11341 cactggcggc gcacagggcg ttcccgcatg gggctgtgag tgcgcggcct gcgccagagc
    11401 gcggcgctca ccgcagtatc gccgccagcc atgcagcggc gtcgtgaagt ttaacgacgc
    11461 gatcaccctg atcgacgccg ggctgcacga tctcgccgat cgctggtcgc ccggatcgtt
    11521 ccagcagttt ttgctgacgc actaccatat ggatcacgtg caggggctgt ttccgctgcg
    11581 ctggggcgtg ggcgacacaa tcccggtcta cggtccaccg gatgagcagg gctgcgacga
    11641 tctgtttaaa catccgggcc tgcttgattt cagccacacg gtggagccgt ttgtggtgtt
    11701 tgatttgcag gggttgcagg tcacgccgct gccgcttaac cattcgaaac tcactttcgg
    11761 ctatttactg gaaacggcgc acagccgggt ggcgtggcta tctgacaccg caggcctgcc
    11821 ggaaaaaacg ctgaaatttt tacgcaataa tcagccgcag gtaatggtga ttgattgcag
    11881 ccacccgccg cgcgaggatg caccgcgtaa tcactgtgat ttaaataccg tgcttgcgct
    11941 gaatcaggtt atccgctcgc cacgggtgat tctgacccat atcagccacc agtttgatgc
    12001 gtggctgatg gaaaatgcac tgccgtcggg gtttgaggcg gggtttgatg ggatggagat
    12061 tgaggtggag tgagggaaaa tacccgcgcc ggcccctacc tcaacgccca tactcatctt
    12121 ccattctccg ctcttcatct tccagttgct gacgctcctg atcaagctgg cgctggcgtt
    12181 cgtccagctg cctgcggcga tcttcaaact ggcggcggcg gtcatcatat tgtctgcgcc
    12241 gatcgtcgct cacttcacgc tgccagccgt cgtcgcgcga atcttcatag tctcgcccac
    12301 ggtcagggtt ataagcgtcg ttaatcgcct gctgaatatt gccaatggtg tcatcgataa
    12361 tatcggcctg ggccggaacg gtgaacagcg tgaacagcgt gaataaaaga aatagcggaa
    12421 agcgtttcat tagccaacct caaaaagaaa ctctatccac attagtcatt actcatccat
    12481 gcaagtagtg gatgaatctc aatttctccg ctgctctatt gccgtaatcg cctccacgcg
    12541 ttgttgatga cgaccgcctt cgtactgtgc gcccagccac gcatcgacaa tcatttttgc
    12601 cagttcgagg ccaactactc gtgaaccaaa agccagcacg ttggtgtcgt tatgctgccg
    12661 cgaaagttgc gcggaataag gttcgctaca aacgaccgcg cgaattccgg caaacttgtt
    12721 cgccgctatc gaaataccga cgccagtgcc acaaatcaaa atcccgccat cagcctctcc
    12781 gccagcaacg gccagtgcga cttgactggc gtaatgcgga taatcagtac gctctgacga
    12841 ccaggttcct ttatcaatca cttcaacgcc acgctcaact aaatgtgcca ctatttcatg
    12901 ttttaaaatg aaaccgacat gatcacagcc aaatgcaatc tttttcattc atcttccccg
    12961 ctcgcgcaaa acttcacaat aaaaaaacat ttgtgattta aatgaattaa aaacacccaa
    13021 aatcgatgaa atcagccttc ccgatctgtt ttacagagtt acatctggca gcgcaatag
//
LOCUS       DACADF010000131        12780 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.133, whole genome shotgun sequence.
ACCESSION   DACADF010000131 DACADF010000000
VERSION     DACADF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.133"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            104..1162
                     /gene="citC"
                     /locus_tag="ISB30_003685"
     CDS             104..1162
                     /gene="citC"
                     /locus_tag="ISB30_003685"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAP1991277.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1177..1473
                     /gene="citD"
                     /locus_tag="ISB30_003686"
     CDS             1177..1473
                     /gene="citD"
                     /locus_tag="ISB30_003686"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAP1991278.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1470..2378
                     /gene="citE"
                     /locus_tag="ISB30_003687"
     CDS             1470..2378
                     /gene="citE"
                     /locus_tag="ISB30_003687"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAP1991279.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2389..3921
                     /gene="citF"
                     /locus_tag="ISB30_003688"
     CDS             2389..3921
                     /gene="citF"
                     /locus_tag="ISB30_003688"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAP1991280.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCADLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSQAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3925..4476
                     /gene="citX"
                     /locus_tag="ISB30_003689"
     CDS             3925..4476
                     /gene="citX"
                     /locus_tag="ISB30_003689"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAP1991281.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4451..5329
                     /gene="citG"
                     /locus_tag="ISB30_003690"
     CDS             4451..5329
                     /gene="citG"
                     /locus_tag="ISB30_003690"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAP1991282.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLNNGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5380..6843
                     /gene="citT"
                     /locus_tag="ISB30_003691"
     CDS             5380..6843
                     /gene="citT"
                     /locus_tag="ISB30_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAP1991283.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            6957..7763
                     /gene="rna"
                     /locus_tag="ISB30_003692"
     CDS             6957..7763
                     /gene="rna"
                     /locus_tag="ISB30_003692"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAP1991284.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            7993..8403
                     /gene="rnk"
                     /locus_tag="ISB30_003693"
     CDS             7993..8403
                     /gene="rnk"
                     /locus_tag="ISB30_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAP1991285.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8759..9187
                     /gene="uspG"
                     /locus_tag="ISB30_003694"
     CDS             8759..9187
                     /gene="uspG"
                     /locus_tag="ISB30_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAP1991286.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9308..10873)
                     /gene="ahpF"
                     /locus_tag="ISB30_003695"
     CDS             complement(9308..10873)
                     /gene="ahpF"
                     /locus_tag="ISB30_003695"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP1991287.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11044..11607)
                     /gene="ahpC"
                     /locus_tag="ISB30_003696"
     CDS             complement(11044..11607)
                     /gene="ahpC"
                     /locus_tag="ISB30_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAP1991288.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            11979..12725
                     /gene="dsbG"
                     /locus_tag="ISB30_003697"
     CDS             11979..12725
                     /gene="dsbG"
                     /locus_tag="ISB30_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAP1991289.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMRNK"
BASE COUNT         3021 a         3377 c         3243 g         3139 t
ORIGIN      
        1 cccccctttc tcccaagtga aaataaaagg ttatcaattt gcaacattga acaacattcg
       61 ttgcaaatcg ataacaacat gcaccttcag gatactattt attatgttcg gcaatgatat
      121 tttcacccgc gtaaaacgtt cagaaaataa aaaaatggcg gaaatcgccc aattcctgca
      181 tgaaaatgat ttgagcgttg acaccacagt cgaagtattt attaccgtaa cccgcgatga
      241 aaagcttatc gcgtgcggtg gaattgccgg aaatattatt aaatgcgttg ctatcagtga
      301 atccgttcgc ggtgaaggac tggcgctgac attagccact gaactgataa acctcgccta
      361 tgagcggcac agcacgcatc tgtttattta taccaaaacc gaatacgagg cgctgttccg
      421 ccagtgcggt ttttccacgc tgaccagcgt acccggcgtg atggtgctga tggaaaacag
      481 cgccacgcga ctgaaacgct atgccgaatc gctgaaaaaa tttcgtcatc cagggaacaa
      541 aattggctgc attgtgatga acgccaatcc ctttacgaat ggtcaccgtt atctgattca
      601 acaggctgcg gcacaatgcg actggttgca tctgttttta gtcaaagaag attcttcacg
      661 cttcccctat gaagaccggc tggatctggt gttaaaaggc accgccgata ttccacgcct
      721 gactgtgcat cgcggctccg aatacatcat ctcccgcgca acgttccctt gctacttcat
      781 taaagaacag agcgtcatta accattgcta caccgaaatt gatctgaaga ttttccgtca
      841 gtacctcgct cccgcgctgg gtgtaactca ccgctttgtc ggtactgaac ccttttgtcg
      901 cgttaccgcc cagtacaacc aggatatgcg ctactggctg gaaacgccga ctatctccgc
      961 accgcccatc gaactagttg aaattgagcg gctgcgttac caggagatgc cgatatccgc
     1021 ttcccgggta cgtcaactgc tggcaaaaaa cgatctcacg gctatcgcgc cgctggtccc
     1081 tgcagtcacg ctgcattatt tgcagaacct gcttgagcac tcccgccagg acgcggcagc
     1141 tcgtcaaaag acccccgcat gagaaacagg tgaaaaatga aaataaacca gcccgccgtt
     1201 gcaggcaccc ttgagtctgg ggatgtgatg atacgcatcg ccccactcga tacgcaggat
     1261 atcgacctgc aaatcaacag cagcgttgag aaacagtttg gcgatgcaat tcgcaccacc
     1321 attctggacg ttctcgcccg ctacaacgtg cgcggcgtac agctgaatgt cgatgacaaa
     1381 ggcgcactgg actgcatttt acgtgcacga ctggaagccc tgctggcacg cgccagcggt
     1441 atcccggctc tgccatggga ggattgccaa tgatttccgc ttcgctgcaa caacgtaaaa
     1501 ctcgcacccg ccgcagtatg ttgtttgtgc ctggtgctaa tgccgcgatg gtcagcaact
     1561 ccttcatcta cccggctgat gccctgatgt ttgacctcga agactccgta gcattgcgtg
     1621 aaaaagacac cgcccgccgc atggtttacc acgcgctgca acatccgctt tatcgcgata
     1681 ttgaaaccat tgtgcgtgta aacgcgctgg attccgaatg gggtgttaac gacctggaag
     1741 ccgtcgttcg cggtggtgcg gacgttgtgc gtctgccgaa aaccgatacc gctcaggatg
     1801 ttctggatat cgaaaaagag atcctgcgta tcgaaaaagc ctgtggtcgt gaacccggca
     1861 gtaccggcct gctggcggcg attgaatctc cgctgggcat tacccgcgca gtggaaatcg
     1921 ctcacgcttc cgagcgtttg atcggtatcg ccctcggcgc agaagactat gtgcgcaacc
     1981 tgcgtacaga acgctccccg gaaggaactg aactgctgtt cgctcgctgc tccattttgc
     2041 aggccgcgcg ctctgcgggt attcaggcgt tcgataccgt ctattccgac gctaacaacg
     2101 aagccggatt cctgcaagaa gccgcccaca tcaaacagct gggctttgac ggcaaatcgc
     2161 tgatcaaccc gcgtcagatt gatctgctgc acaacctcta cgcaccgacc cagaaagaag
     2221 tggatcacgc ccgccgcgtc gtagaagccg ctgaagccgc cgctcgcgaa ggcctcggcg
     2281 tggtttccct gaacggcaag atggtggacg gtccggttat cgatcgcgcc cgtctggtgc
     2341 tctcccgtgc agaactttcc ggcatccgcg aagaataagg caatcaaaat gacgcagaaa
     2401 attgaacaat ctcaacgaca agaacgggta gcggcctgga atcgtcgcgc tgaatgcgat
     2461 cttgccgctt tccagaactc gccaaagcaa acctaccagg ctgaaaaagc gcgcgatcgc
     2521 aaactgtgcg ccgatctgga agaagcgatt cgtcgctctg gtttacagga cggcatgacg
     2581 gtttccttcc atcacgcttt ccgtggcggt gacctgaccg tcaatatggt gatggacgtc
     2641 atcgcgaaga tgggctttaa aaacctgact ctggcctcca gctccctgag tgattgccat
     2701 gcgccgctgg tagaacacat tcgccagggc gtggttaccc gcatttatac ctctggcctg
     2761 cgcggtccac tggcggaaga aatctcccgt ggtctgctgg cggaaccggt gcaaatccac
     2821 tctcacggcg gacgtgtgca tctggtacag agcggcgaac tgaatatcga tgtggctttc
     2881 ctcggcgtcc cgtcctgtga tgaattcggt aatgccaacg gctactccgg taaagcctgc
     2941 tgcggctccc tcggctatgc aatggttgat gccgacaacg caaaacaggt cgtgatgctt
     3001 accgaagaac tgctgcctta tccgcataat ccggcaagca ttgagcaaga tcaggttgat
     3061 ctgatcgtca aagttgatcg cgttggcgat gctgcaaaaa tcggcgctgg tgctacccgt
     3121 atgaccacta acccgcgcga actgcttatt gcccgtagcg ctgcggatgt gattgttaac
     3181 tctggctact tcaaagaagg tttctccatg cagaccggca ccggcggcgc ttcgctggcg
     3241 gtaacccgtt tcctggaaga caaaatgcgt agccgcgata ttcgcgccga cttcgccctc
     3301 ggcggtatta ccgcgacgat ggttgacctg cacgaaaaag gtctgatccg caaactgctg
     3361 gatgtgcaga gctttgacag ccaagctgcg caatcgctgg cccgtaaccc caatcacatc
     3421 gaaatcagcg ccaaccaata cgctaactgg gggtcgaaag gcgcatcggt tgatcgtctc
     3481 gacgtggtgg tactgagtgc gctggaaatt gacacccagt tcaacgttaa cgtgctgacc
     3541 ggctctgacg gcgtactgcg tggtgcttcc ggtggtcact gcgataccgc gattgcctct
     3601 gcgctttcca tcatcgtcgc gccgctggta cgcggtcgta ttccgacgct ggtggataac
     3661 gtgctgacct gcatcacccc gggctccagt gtcgatattc tggtcacaga ccacggcatc
     3721 gctgttaacc cggcacgtcc ggagctggca gaacgtctgc aggaagcggg gatcaaagtg
     3781 gtttctattg agtggctgcg tgaacgtgcg cgtctgctga ccggtgaacc acagccgatt
     3841 gaattcaccg accgcgtcgt tgccgttgtg cgttaccgcg atggctcggt gatcgatgtt
     3901 gtgcatcagg tgaaggaata agccatgcac ctgcttcctg aactcgccag ccaccatgcg
     3961 gtgtcaattc ccgagctgct cgtcagccgg gatgaaaggc aagcacggca acacgcctgg
     4021 ctcaagcgcc atcctgttcc actggtctcc tttaccgtgg ttgcgccagg gccgattaaa
     4081 gacagcgagg tcacacgccg aatttttaat catggcgtga cagccttgcg tgccttagcc
     4141 gcaaaacagg gctggcaaat tcaggagcag gctgcactgg tttccgccag cgggccggag
     4201 ggcatgttga gcattgccgc cccggctcgc gacctcaagc tcgccaccat tgagcttgaa
     4261 catagtcatc ctctcgggcg gttatgggat atcgatgtcc tgacgcccga aggcgacatt
     4321 ctctcccgcc gcgattattc actgccgcct cgccgctgcc tgttgtgcga gcaaagcgca
     4381 gctgtttgcg cgcgtggaaa aacccatcaa ctgaccgatt tactcaaccg catggaggcg
     4441 ctgctaaacg atgtcgatgc ctgcaacgtc aactaaaacc acaaagcttg cgacgtcatt
     4501 aattgatgag tacgccctgc tgggctggcg cgccatgctg actgaagtca acctgtcacc
     4561 gaaaccaggc ctcgtggatc gcattaactg cggtgcgcac aaagatatgg cgctggaaga
     4621 tttccaccgc agcgcgctgg cgattcaggg ctggttaccc cgtttcattg aatttggtgc
     4681 ctgtagtgca gaaatggcac cggaagcggt actcaacgga ttacgcccaa ttggtatggc
     4741 ttgcgaaggt gatatgttcc gcgccactgc gggcgtaaac acgcataaag gaagcatttt
     4801 ttctttaggg ctactgtgtg cggcaattgg ccgtttgctt cagctcaacc agccggtaac
     4861 gccaacaacc gtttgttcta cggcggcaag tttctgccgt ggcctgaccg atcgcgaact
     4921 gcgtaccaat aattcacgac tgacggcagg tcaacggttg taccagcagc ttggccttac
     4981 cggcgcacgc ggtgaagccg aagcaggtta tccactggta atcaaccacg ccttgccgca
     5041 ttacctcact ctgctggatc aggggttaga tcctgaactg gcactgctcg ataccttgct
     5101 cctactgatg gctaccaacg gcgataccaa cgttgcatcg cgcggtggcg aggggggcct
     5161 gcgctggcta cagcgcgagg cgcaaacact attaaacaat gggggcatcc gaacccccgc
     5221 cgatctcgat tatctccggc agttcgacag ggagtgtatc gaacgaaatc tcagtccagg
     5281 cggcagtgcc gacctgctga ttcttacctg gtttttagca cagatttaat tatttaagca
     5341 cttgataaat ttggaaatat taattttcgg agaacccgta tgtctttagc aaaagataat
     5401 atatggaaac tattggcgcc cctggtggtg atgggtgtca tgtttcttat ccctgtacct
     5461 gacggtatgc cgccgcaggc gtggcattac tttgcagtgt ttgtggcaat gattgtcggc
     5521 atgatcctcg agccaattcc ggcaacggcg atcagtttta ttgcggttac tatttgcgtt
     5581 attggcagta attacctgct ctttgatgcc aaagaattag ctgacccagc gtttaatgcg
     5641 caaaaacagg cgctgaaatg gggtctggct ggtttttcca gcaccacggt atggctggta
     5701 tttggcgcat ttatttttgc attagggtat gaagtttccg ggttaggccg tcgtattgcc
     5761 cttttcctgg tgaaattcat ggggaaacgc acgctgacgt tgggttatgc gattgtcatt
     5821 atcgacattc tgctggcacc gtttacaccg tccaacaccg cgcgtaccgg gggtacggtt
     5881 tttccggtca ttaaaaacct gccgccgctg tttaaatcat tcccgaacga tccgtccgcg
     5941 cgtcgtattg gcggctattt gatgtggatg atggtcatta gtaccagtct gagttcgtcc
     6001 atgtttgtca ccggtgcggc accaaacgtg ctgggtctgg agttcgtcag caaaattgcc
     6061 ggtatccaga ttagctggtt gcagtggttc ctctgcttcc tgccggttgg ggttatcttg
     6121 cttatcattg cgccgtggct ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa
     6181 gaagtggcaa cctgggcggg tgatgaacta aaaaccatgg gtgcgctgac gcgcagagag
     6241 tggacgctga ttggccttgt attgctcagc ttaggtttgt gggtatttgg cagtgaagtc
     6301 attaatgcta ctgcggtagg tctgctggca gtttcgctga tgctggcgct gcacgttgtg
     6361 ccgtggaaag acattactcg ctataacagc gcatggaaca cgctggtcaa cctggcaacg
     6421 ctggttgtta tggcgaatgg cctgacccgt tctggcttta tcgactggtt cgccaacacg
     6481 atgagtacac acctggaagg cttctcacca aacgcaacgg tgattgtact ggttctggtg
     6541 ttctactttg cacactacct gtttgccagc ctgtctgcgc acaccgcaac catgctgccg
     6601 gttattctgg ccgtcggtaa agggattccg ggcgtaccaa tggaacaact gtgtatcctg
     6661 ctggtgctgt ctatcggtat catgggctgt ctgacgcctt atgcaaccgg tcctggggtg
     6721 attatttacg gctgtggcta tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc
     6781 ggggtgattt acatctctat gctgctgttg gttggctggc cgattctcgc catgtggaac
     6841 taatacgttc gccaaacgct gaataaatca tcacgcccgc caggtaagcc cacctggcgg
     6901 gcttttttat gatttaatag ataatattca gtgtatgtat gagttccaca cccattatga
     6961 aagcattctg gcgtaacgcc gcgttgctcg cggtttctct gcttcccttc tcttctgcca
     7021 acgccgtggc gttgcaggca aaacagtatg gcgattttga tcgctatgtc ctggccctct
     7081 cctggcaaac cggattttgc cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat
     7141 gtcgcctgca aaccgaaacg accaacaaag ctgatttttt gaccgtacat ggtctgtggc
     7201 caggattgcc taaatcggtt gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg
     7261 gttgcgctac tcgcccaatc ccgaatctgc cagaagcacg cgccagccgg atgtgttcat
     7321 cgccggaaac cgggttatcg ctggaaacgg cagctaaact aagtgaagtc atgccaggag
     7381 ctggcggacg ttcctgcctg gaacgctacg aatatgccaa acacggtgcc tgctttggtt
     7441 ttgatccaga cgcatacttc ggcacgatgg tacgcctgaa tcaagaaatt aaagagagcg
     7501 aagccggaaa attccttgcg gataactacg gtaaaacagt gagccgccgt gactttgatg
     7561 ccgcctttgc caaaagctgg ggaaaagaga acgtgaaagc agttaagcta acgtgccagg
     7621 gtaaccctgc gtatttgact gaaattcaga tctcgatcaa agctgacgcc atcaacgctc
     7681 cgctttctgc caactcattc ttgccacaac cacacccagg caactgtggc aaaacctttg
     7741 tgattgataa agcaggttat taatcctctg atttacaaaa cacgctaatt cgtcttccat
     7801 aaaccatgac gattaagatt tatacataat gcgctacatt gaggaattcc ctgaatgtga
     7861 cgcaaatcac ttcaaagagt gagcaacagt cagtatcatc atgtgagtta aaccctcgcc
     7921 gcctgacggt gagggttttc ttttgggtat gtttatctgc gacactcgca gtaccgacga
     7981 catggagtaa aaatgtccag accaactatc atcattaacg acctggatgc cgaacgcatc
     8041 gatattctgc tggagcaacc cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca
     8101 gagttggatc gcgcccaaat gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg
     8161 aacagccggg ttaaattccg caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg
     8221 tatccggcaa aaatgaccga tagcaatact cagctttccg ttatggctcc ggtaggtgcc
     8281 gcactgctgg ggctgcgcgt tggcgattcc attcactggg aacttccggg cggcgttgca
     8341 acccaccttg aagtgctgga actcgaatac cagccagaag ctgctggcga ctacctgctt
     8401 taatcccatc tgcacaagcg ataactcgct ttgcccagag gatgcactcg catcctcttt
     8461 ccgctcaaat atcaaccaga tttatcattt tgctcttaag cgcagcattc taatctgaac
     8521 tggttattca tctgcggaac ctcatatgag cgaagctctc ggtatcactg cattttatat
     8581 attcatagca gcaattattg ttgctgcagt gctatacctt gaacaacgct ggtagcgcct
     8641 ggttttattt attttccagc tcaatagcaa attcagatca aatcagcctc gcaaattata
     8701 gattcataac caataatgtg ctgttatgta tacatgcaaa aacaaggaga gaatgcttat
     8761 gtacaaaaca atcatcatgc cagtggatgt tttcgaaatg gaattgagcg acaaagcgat
     8821 tcgccacgct gaattcctcg cccaggatga cggagttatt catctacttc acgtactacc
     8881 ggggtcagcc agcctgagcc tgcaccgttt tgccgctgat gtacgtcgtt ttgaagagca
     8941 tctgcaacat gaagcagaag aacgtttgca aacgatggtc agccacttca ccatcgatcc
     9001 ttcccgcatt aagcaacatg tccgttttgg tagtgtgcgg gatgaagtta acgagttagc
     9061 gaaagaactt gatgcagatg tagtagtgat tggttcgcgt aacccatcaa tttcaactca
     9121 tctgttgggt tctaacgcct cgagcgtaat ccgccacgcc aatttgccgg tgctggttgt
     9181 gcgttaattg ccagaataag tctcccgccc tgcccggagc catccctgca gggcttttta
     9241 atgccataaa aaagccgcca ggtttgaccc cggcggctaa gcaattacag gtgaatctta
     9301 cttcttctta tgcagttttg gtgcgaatca ggtagtcaaa agcactcaga gaggctttgg
     9361 caccttcgcc agtggcgatg atgatctgct tgtacggaac cgtcgtacag tcacccgctg
     9421 cgaacacgcc tttgacgttg gtttcgcatt tcgcatcaat gataatctcg cccatgcgat
     9481 tacgttcgac tgcgccttcc agccagttgg tgttcggcag cagaccaatc tggacgaaaa
     9541 taccggccag ttcgatgttg tgaatatcgc cgctgacacg atcgcgatat tccagaccta
     9601 cgactttgct accgtcgcct ttcacttccg tggtttgcgc attcagaata atgtcgacgt
     9661 ttttcaggct gcgtagtttg tcctgcagaa cctggtcggc tttcatttct ggcgcaaatt
     9721 ccagcagcgt tacgtgctca acgatacccg ccaggtcaat tgccgcttcc acgccggagt
     9781 taccgccgcc gataaccgct acgcgtttgc ctttaaacag cgggccgtcg cagtgcgggc
     9841 agtaggtcac gcctttggtg cgatactgat cttcgccagg aacgttcatg ttgcgccatt
     9901 ttgcaccagt cgccacgata atgctgcgtg ctttcagtac cgcgccagaa gctgtttcaa
     9961 tctgatgcag gccgccttca accgctgccg ggatcagttt gctcgcgctc tggctgtcga
    10021 tcacatcaac gtcgtattca tcaacatgaa ctttcagcgc acctgccagt ttctggcctt
    10081 cggtcttcgg tacagaaatg tagttttcga tatcaacggt atcgaggatc tgaccaccaa
    10141 aacgttcgcc catcagaccg gtacggatgc ctttacgtgc ggagtaaatt gctgccgctg
    10201 cacccgcagg gccggaacca acgattaata cgtcataagc atcacgcttg ttcagctctt
    10261 ctgccgcacg tttttccgca ccagtatcaa ttttggcaac gatttcagtc aacgtcatgc
    10321 ggccctgacc aaactctttc ccgtttacga acactgccgg aacgcccatc acgttgcgat
    10381 cggtgatttc gttctggaag gtgccgccgt caattgcagt gtgcttgatg cgcgggttca
    10441 gtacgctcat caggttcagc gcctgcacca cgtccgggca gttgtggcaa gagagcgaat
    10501 aataggtttc gaattcaaaa tcaccgtcaa tatggcgaat ctgctccagc agagactgcg
    10561 cttctttcga cggatgacca ccggtccaca acaacgccag taccagcgag gtgaactcgt
    10621 ggcccagcgg ggaacctgca aaacgtggcc cctggttgga accagggttg gtaatcagga
    10681 aagacggctt acgcaccggc aagctgttat cttctttaaa ggtgactttg tctgacagtt
    10741 ctgcgatttc agccaacagt tccttgattt ctgccgattt agcgctgtca tccagcgtgg
    10801 caattaactc aacaggcttg gtcaatttct caaggtaagc cttgagttga gttttcatat
    10861 ttgtgtcgag catgtttatc tcctgggctt taaacatcat catgcaagcg gcctctgcgg
    10921 ctagctggat gcagcttgca tcatggtgcg gggaagaaaa tcgggtgccg cagcacccga
    10981 agaattaggt gaatttccga agtatctcgc gccggagttg atagcgcgaa agacgaagga
    11041 aatttagatt ttaccaacca ggtccagaga cggagccaga gttgcttcac cttctttcca
    11101 tttagccggg caaacttcac ctgggtgaga agctacgtac tgtgctgctt tgattttacg
    11161 cagcaggtca gacgcgtcac ggccaatgcc ttcagcggta acttcgattg cctggatgat
    11221 accctgcggg tcaacaacga aggtcgcacg gtcagccaga ccttcatctt cacgcatgtt
    11281 gtcgaagtta cgggtcaggg cgccagtcgg gtcgccgatc atcgcatatt tgattttagc
    11341 gatggtttca gagctgctgt gccatgcttt gtgggtgaag tgagtatcgg tagatactgc
    11401 gtatacgtct acgcccagtt tctgcagttc ttcgtaatgg tcagcaacgt cacccagttc
    11461 ggtcgggcat acgaaagtaa agtcagccgg gtagaagaag aagacgctcc agcggccttc
    11521 ggtatctttt tcggtgattt cgatgaattc gccgtttttg aatgcctggt ttttaaaagg
    11581 tttaattttg gtgttaatca aggacatcta tacttcctcc gtgttttcga tgagatgtaa
    11641 ggtaaccaat ttttgccgat tcggctaatg cgtttccatt atcaaatcga taagttttac
    11701 cttacaacct tcgtaagaca actttgtgaa ctttgcaccg ccagcggcga taaaaagtaa
    11761 aatggccccc tttccaggtg accgtgtaat ctgaactacc ctttggtatc ttcagcacct
    11821 gcctgcaggc tgaggtgttg cccttacaaa tgcaacaacg acatggatta caacaccctc
    11881 ataaacaaag ggcaatcacc tgatctaagc tcttacctat gacagtgata ggttatgcct
    11941 tttactcgac ttttgcactg actgaaaagg acaaattaat gttaaaaaag atacttttac
    12001 tggccctgct tcctgcaatc gccttcgcag aggaacttcc tgccccagta aaagcgattg
    12061 aaaaacaggg tattacaatc atcaaaacgt tcgatgcccc tggaggaatg aaaggttatc
    12121 tcggtaagta tcaggatatg ggcgtcacca tctacctgac tccagatggt aagcacgcta
    12181 tctctggtta catgtacaac gaaaaaggtg aaaacctgag taacacgctt atcgaaaaag
    12241 aaatttacgc acccgccgga cgtgaaatgt ggcaacggat ggaacaatcc cactggctcc
    12301 tcgacggtaa aaaagacgcg ccggtcattg tctacgtctt cgccgatccg ttctgcccat
    12361 attgtaaaca gttctggcag caggctcgtc cgtgggtaga ttctggcaaa gtgcaattga
    12421 gaacattgtt ggttggggtt atcaagccag aaagcccggc gacagcagcg gcaattcttg
    12481 cctccaaaga tcccgcaaaa acctggcaac aatatgaagc ctctggtggc aagcttaagc
    12541 taagcgtgcc tgcaaacgta agcacagagc aaatgaaagt gttaagcgac aacgagaaac
    12601 tcatggacga tctgggggca aatgtcacac cagctatcta ttacatgagt aaggaaaata
    12661 ccctgcaaca agccgtcgga ttacccgatc agaaaacgct taatatcatt atgaggaata
    12721 aataaaagat aattcatgga tggttgaaat acatcattgc ttttcaacca tcctaaaaac
//
LOCUS       DACADF010000132        12621 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.134, whole genome shotgun sequence.
ACCESSION   DACADF010000132 DACADF010000000
VERSION     DACADF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.134"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            35..1549
                     /gene="caiT"
                     /locus_tag="ISB30_003698"
     CDS             35..1549
                     /gene="caiT"
                     /locus_tag="ISB30_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HAP1991290.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSVLVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKY"
     gene            1580..2722
                     /gene="caiA"
                     /locus_tag="ISB30_003699"
     CDS             1580..2722
                     /gene="caiA"
                     /locus_tag="ISB30_003699"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAP1991291.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2851..4068
                     /gene="caiB"
                     /locus_tag="ISB30_003700"
     CDS             2851..4068
                     /gene="caiB"
                     /locus_tag="ISB30_003700"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAP1991292.1"
                     /translation="MDHLTMPKFGPLSGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4141..5694
                     /gene="caiC"
                     /locus_tag="ISB30_003701"
     CDS             4141..5694
                     /gene="caiC"
                     /locus_tag="ISB30_003701"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAP1991293.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDIYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSVRAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSVNDRQHCLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5803..6588
                     /gene="caiD"
                     /locus_tag="ISB30_003702"
     CDS             5803..6588
                     /gene="caiD"
                     /locus_tag="ISB30_003702"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAP1991294.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6594..7184
                     /gene="caiE"
                     /locus_tag="ISB30_003703"
     CDS             6594..7184
                     /gene="caiE"
                     /locus_tag="ISB30_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAP1991295.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVLAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7303..7698)
                     /gene="caiF"
                     /locus_tag="ISB30_003704"
     CDS             complement(7303..7698)
                     /gene="caiF"
                     /locus_tag="ISB30_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAP1991296.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7959..11180)
                     /gene="carB"
                     /locus_tag="ISB30_003705"
     CDS             complement(7959..11180)
                     /gene="carB"
                     /locus_tag="ISB30_003705"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP1991297.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11198..12346)
                     /locus_tag="ISB30_003706"
     CDS             complement(11198..12346)
                     /locus_tag="ISB30_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase small subunit"
                     /protein_id="HAP1991298.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
BASE COUNT         2960 a         3289 c         3339 g         3033 t
ORIGIN      
        1 aaataaatat tcgttttcac agtggaatta actcatgaag aatgaaaaga gaaaaacggg
       61 aatagaaccg aaggttttct ttccgccgtt aataatcgtc ggcatacttt gttggcttac
      121 agtcagagat ctggatgcag cgaatgtcgt tattaatgct gtattcagtt acgtcaccaa
      181 tgtatgggga tgggcatttg aatggtatat ggtggtgatg cttttcggct ggttctggct
      241 ggtgtttggc ccgtatgcca aaaagcgttt aggtaacgaa ccaccagaat ttagtaccgc
      301 cagttggatc tttatgatgt tcgcctcctg tacgtctgct gccgtactgt tctggggatc
      361 gattgagatc tactactaca tctccacccc gccgtttggc ttagaaccga actcaacagg
      421 cgcgaaagag ttggggctgg cttacagctt gttccactgg ggacctctgc cgtgggccac
      481 ttacagcttc ctttcagtcg ccttcgctta cttcttcttt gtccgcaaaa tggaagtgat
      541 tcgccccagc tcgaccctgg tgccgctggt aggtgaaaaa cacgccaaag ggttgttcgg
      601 cactatcgtc gacaacttct atctcgtcgc cttgatcttc gcgatgggta ccagtctggg
      661 ccttgccacg ccgctggtaa ccgagtgtat gcaatggttg tttggcattc cgcataccct
      721 gcaactggac gctatcatca ttacctgctg gattatcctc aacgccattt gcgtcgcttg
      781 cggtctgcaa aaaggggtac gtatcgccag tgacgtgcgt agttacctga gcttcctgat
      841 gctgggttgg gtgttcatcg tcagcggtgc cagcttcatc atgaactact tcaccgattc
      901 ggtggggatg ttgctgatgt atctgccgcg catgttgttc tataccgatc ccatcgctaa
      961 aggcggcttc ccgcagggct ggaccgtgtt ctactgggca tggtgggtga tttacgctat
     1021 ccagatgagt atcttcctcg cccgcatctc ccgtggtcgt accgtgcgtg aactgtgctt
     1081 cggcatggtg ctggggctga cagcgtcaac ctggatcctg tggactgtac tcggtagtaa
     1141 caccctgctg ttgatagata aaaacatcat caacattcca aatctgatcg aacagtacgg
     1201 tgtggcgcgc gccatcatcg aaacctgggc cgctctgcca ctcagcaccg ccaccatgtg
     1261 gggcttcttc atcctctgct ttattgccac cgttacgctg gttaacgcct gctcttatac
     1321 cctggcgatg tccacttgcc gcgaagtacg tgacggcgaa gaacccccgc tgctggtgcg
     1381 cattggttgg tcagttctgg ttggcattat cggcattgtt ctgctggcgc tcggcggcct
     1441 gaaaccgatt caaaccgcca ttatcgccgg aggatgcccg ctgttcttcg tcaacatcat
     1501 ggtgacgctc tcctttatta aagacgcgaa acagaactgg aaatattaat taaccccaaa
     1561 aatatcaaga ggttgaaaga tggattttaa tttaaatgat gagcaggaac tgtttgtcgc
     1621 cggtatccgc gaactgatgg ccagcgaaaa ctgggaggcc tattttgccg agtgcgaccg
     1681 tgacagcgtc tacccggaac gttttgtcaa agccctggcg gatatgggta tcgacagtct
     1741 gctgatccct gaagagcacg gtggtctgga cgcggggttt gttactctcg ccgccgtgtg
     1801 gatggaactg ggtcgtctgg gagcgccaac ttacgtgctg tatcagttac cgggcgggtt
     1861 taacaccttc ctgcgtgaag gtacgcaaga gcagatcgac aagatcatgg ctttccgcgg
     1921 caccggtaag cagatgtgga actcagcgat taccgaaccg ggcgcaggct ccgacgtggg
     1981 tagcctgaaa acgacttata cccgtagaaa tggtaagatt tatcttaatg gtagtaagtg
     2041 ttttattacc agcagcgcct acaccccgta catcgtggtg atggcgcgcg acggggcttc
     2101 tccggacaaa cctgtctaca ccgaatggtt tgttgatatg agcaaaccgg gcatcaaagt
     2161 gaccaaactt gagaagctcg gtctgcgtat ggatagctgc tgtgaaatca cctttgacga
     2221 tgtggaactg gacgagaaag acatgttcgg tcgggaaggt aacggcttta accgcgtcaa
     2281 agaagagttc gaccatgaac gtttcctggt agccctgacc aactacggta cggcgatgtg
     2341 cgcctttgaa gatgcggcgc gctacgccaa ccagcgcgtg cagtttggcg aggctattgg
     2401 tcgtttccag ttgattcagg aaaaattcgc ccacatggcg atcaaattaa actccatgaa
     2461 aaacatgctg tatgaagcag cgtggaaagc agacaacggc accatcacct ctggcgatgc
     2521 agcgatgtgc aaatacttct gcgccaatgc ggcatttgaa gtggtggata gcgcaatgca
     2581 ggtgctgggc ggtgtcggga ttgcgggtaa tcaccgcatc agccgcttct ggcgtgacct
     2641 gcgtgtagac cgcgtttccg gaggatctga cgaaatgcag atcctgacgc tgggccgcgc
     2701 ggtgctgaag caataccgct aagtccgtgc gctgcctgat gcgacgctga cgcgtcttat
     2761 caggcctaca ggagcacgaa tgtcggccgg ataaggcgtt tacgccgcat ccggcagtca
     2821 tgcgctacat aaatttttca ggagctaata atggatcatc taaccatgcc gaaattcggg
     2881 ccgctgtccg gattgcgcgt tgtcttctcc ggtatcgaaa tcgccggacc gtttgccgga
     2941 caaatgttcg cagaatgggg cgcggaagtt atctggatcg aaaacgtcgc ctgggccgac
     3001 accattcgcg ttcaaccgaa ctacccgcaa ctttcccgcc gcaacctgca cgcgctgtcg
     3061 ttaaatatct tcaaagatga aggtcgtgaa gcgtttctga aattaatgga aaccaccgat
     3121 atcttcatcg aagccagtaa aggtccggcc tttgcccgtc gtggcattac cgatgaagtc
     3181 ctgtggcagc acaacccaaa actggttatc gctcacctgt ccggttttgg tcagtatggc
     3241 accgaggagt acaccaatct tccggcctat aacactatcg cccaggcctt cagtggttac
     3301 ctgattcaga acggtgatgt tgaccagcca atgcctgcct tcccgtatac cgccgattac
     3361 ttttctggcc tgactgccac cacggcggcg ctggcagcac tgcataaagt gcgtgaaacc
     3421 ggcaaaggcg aaagcatcga catcgccatg tatgaagtga tgctgcgtat gggccagtac
     3481 ttcatgatgg attacttcaa cggcggcgaa atgtgcccgc gcatgaccaa agggaaagat
     3541 ccctactacg ccggttgcgg tctgtataaa tgcgccgacg gctacatcgt gatggaactg
     3601 gtgggcatta ctcaaattgc agagtgcttt aaagatattg gcctcgcaca tctgctcggc
     3661 acgccagaaa ttccggaagg cacccagctt atccaccgta tcgaatgccc ttacggccca
     3721 ctggtagaag agaaactcga tgcctggctg gcggcacata ccatcgcaga agttaaagaa
     3781 cgctttgccg aactgaatat cgcctgtgcc aaagtactga ccgtaccgga actggaaagc
     3841 aatccacagt atgtggctcg cgaatcaatc actcagtggc aaacgatgga tggtcgcacc
     3901 tgcaaagggc cgaatatcat gccgaaattc aaaaataacc ccggacaaat ctggcgcgga
     3961 atgccctcac atggcatgga cacggctgcc attttgaaaa atatcggcta cagcgaaaac
     4021 gacattcagg agttggtcag caaaggtctg gccaaagttg aggactaaac gcgtggcgtc
     4081 agtcagcttg cctgactagt gcccgctcaa taacaaacga acaaaatgga tagaggtgca
     4141 atggatatca ttggcggaca acatctacgt caaatgtggg acgatctggc ggacgtttac
     4201 ggtcataaaa cggcgctgat ttgtgaatcc agcagcggag tcgttaaccg gtatagttat
     4261 cttgagttaa atcaggagat taaccgcacg gcaaacctgt tttatacgct ggggattcgc
     4321 aaaggcgaca aggttgctct acatctcgac aactgcccgg aatttatctt ttgctggttc
     4381 gggttggcaa aaattggcgc gattatggtg ccgattaacg cccgcctgtt gcgcgaagaa
     4441 agcacgtgga tcctgcaaaa tagccaggcg tgcctgctgg tgaccagtgc gcaattttat
     4501 cctatgtatc aacagattca gcaggaagat gcctctcagt tgcggcacat ttgcctgaca
     4561 gatatggcac ttcccgctga tgatggcgtg agttcgttta ctcaactgaa aaatcaacaa
     4621 cctgccacct tgtgctatgc accgccgcta tcgactgacg atacggcgga aattctcttc
     4681 acctccggca ccacctcccg accgaaaggg gtggtgataa ctcattacaa cctgcgcttc
     4741 gctggatatt actccgcctg gcagtgcgca ctgcgtgatg atgacatcta cctgacggta
     4801 atgcctgcat ttcatatcga ttgccagtgt actgcggcaa tggcggcatt ttctgccggg
     4861 gccacctttg tgctggtcga gaaatacagc gtccgcgcct tctgggggca ggtacagaag
     4921 taccgtgcca ccgttaccga atgtattccg atgatgatcc gtaccctgat ggtacaacca
     4981 ccttctgtga atgacaggca acactgcctg cgggaagtga tgttttatct caacctgtca
     5041 gagcaggaaa aagacgcttt ttgtgaacgc ttcggcgtcc gcttgctgac gtcttacggg
     5101 atgacggaaa ccattgttgg cattatcggc gatcgccctg gcgataaacg acgctggccg
     5161 tcaattggtc gggcggggtt ttgctacgaa gcggagatcc gcgacgatca caatcgcccg
     5221 ctcccggcag gtgaaatcgg tgaaatctgt attaaaggcg taccagggaa aaccatcttc
     5281 aaagagtatt ttctcaaccc gaaagccact gcgaaagtgc tggaagccga tggctggcta
     5341 cataccggcg ataccggata ccgcgacgaa gagggctttt tttatttcgt cgatcgccgc
     5401 tgcaatatga tcaaacgcgg tggcgagaat gtctcctgcg tggagctcga aaatatcatc
     5461 gccacccatc ccaaaattca ggatatcgtg gttgtgggta ttaaagattc gattcgcgat
     5521 gaagccatca aagcatttgt ggtgctgaat gaaggtgaaa cattgagcga agaggaattt
     5581 ttccgcttct gcgaacaaaa tatggcgaaa tttaaagtgc cctcttatct ggagatcaga
     5641 aaagatctgc cacgtaattg ctcggggaaa ataattagaa agaatctgaa ataaatgaaa
     5701 cggcagggaa ctaccctgtc agctaataat cacgctctaa aacaatatgc attttttgca
     5761 gggatgttgt cgtccctgaa aaagcaaaaa tggagaaaag gaatgagtga atcattacat
     5821 ctgacccgca atggatcaat tctggaaatt acccttgatc gtccaaaagc gaatgctatt
     5881 gatgcaaaaa ccagctttga aatgggcgaa gtatttctaa atttccgtga cgatccgcaa
     5941 ttacgtgtcg ccattattac cggtgccgga gagaaattct tttccgcggg ctgggattta
     6001 aaagcggcag cagaaggcga agcaccggat gctgactttg gcccgggagg ttttgcggga
     6061 ttaaccgaaa ttttcaatct cgacaaaccg gtcatcgccg ctgtgaacgg ctatgccttt
     6121 ggcggcggct ttgaactggc gctggcggca gattttattg tttgtgccga taacgccagc
     6181 ttcgccctac cggaagccaa actgggcatc gttcctgaca gcggcggcgt gctgcgtctg
     6241 ccgaagatcc tgccgcctgc catcgtcaat gaaatggtga tgaccggcag acgaatgggc
     6301 gcagaagagg cgctgcgttg gggggtagtc aaccgcgtgg ttagccaggc ggaactgatg
     6361 gataacgccc gcgaactggc gcagcagctg gttaacagcg ctccgctggc gattgcggcg
     6421 ctgaaagaga tctaccgcac caccagcgaa atgccggtag aagaagcgta tcgctatatt
     6481 cgcagcggcg tgttgaaaca ctatccatcg gttctgcatt cggaagatgc cattgaaggg
     6541 ccgctggcgt ttgccgagaa gcgcgatcca gtgtggaaag gacgttaaca accgtgagct
     6601 attacgcctt tgaggggtta attccggttg ttcacccgac ggcgtttgtc catcccagtg
     6661 cagtattgat tggcgatgtg attgtgggag ccggtgtcta catcggccca ctcgcctcac
     6721 tgcgtggtga ctacgggcgg ttgatcgtgc tagcgggagc caatattcag gatggctgca
     6781 ttatgcatgg ctactgcgac actgacacca tcgttgggga aaacggtcat atcgggcacg
     6841 gagcgatcct gcatggttgt gtgattggtc gcgatgcact ggtcgggatg aacagcgtga
     6901 ttatggatgg cgcggtcatt ggcgaagaga gcattgttgc cgctatgagc tttgtcaaag
     6961 cgggctttcg cggcgagaaa cgccagttgt tgatgggtac gccagcccgc gctgtacgca
     7021 gcgtgagtga cgacgagtta cactggaaac ggttgaatac caaagagtat caggatcttg
     7081 tcgggcgctg tcatgcatcg ttacatgaaa cgcagccact gaggcaaatg gaggaaaatc
     7141 gcccccgttt gcaggggacg acggatgtga cgccgaaacg gtgatcttgc cggatgcggc
     7201 gtaaacgcct tatccgacct ggctggaaag caaccgtagg ccggataaga tgcgccagca
     7261 tcgcatccgg caaccaggct gcgtggtatc aacgaaatga cattaacgac gcatactctt
     7321 tgacaacatc atctgccact tctgctcgcg gttaagttcc gtgagcggaa cggcaggaat
     7381 acgcggcgtc tttcttacgc cgactaattt atcccggctg ttattgcgca gacgcgcgta
     7441 aatttgctca tcgatatcaa ccactttaac cagtcgctga cactggcatc cccgcccttc
     7501 cagcttatta gggatcatct taacttcgca gcttatttct gtgacttccg acagaatata
     7561 agtcagggta ttaaccgcct tgctgtgttc aatatcgaaa tgaatagaga tctctcttgc
     7621 tatcacccac cgattttcag ccatcatcca ttcggcgatt aacaagtaga gtggtttttc
     7681 aacatatcct tcacacatac gaactccagt ttcacaaatt aaaagactgg ctatgactcg
     7741 ttttgcagca attaaacaag gccatattaa aattgtggat atatcgatag tatgcaactt
     7801 caccgtcgat aagtgtgagc taaatcctgc ttatgtcatc aaaaatatcg attccccaaa
     7861 agatttactt aatgggcaaa aaaaacggtc ctcatcagag aaccgtatta gttatttgat
     7921 caaattagcg gatgaaaaat atccgccatg acacgctatt atttgatctg cgcgtgcatt
     7981 tcctgcaccg aaattacttt ttcggtcgca tcggcattca gcgccatcgc ggtggcaaag
     8041 ccgccgttca gggtggtgtc gtaatgcact ttatattgca gcgcactgcg acggatcacg
     8101 cgggagtctt caatcgcacg acgacctgag gtggtgttga tgatgtaggt atattcgcca
     8161 ttcttaatac ggtcctgaat gtgcggacgg ccttcatgca ccttgttcac cagacgcgga
     8221 ttgatacccg cttcgcccag cacaatcgcc gtgccgtgag tcgcatccag ctcgaagccc
     8281 tgtttcagca gttttgccgc caggtccacc acgcgttctt tatcgccttc gcgcacggaa
     8341 agcagcgcac gaccgtgttt cttcatggtg gagttgctgc ccagctgcgc tttggcaaac
     8401 gcttcagcga aggtgcggcc cacgcccatg acttccccgg tagagcgcat ttctggccct
     8461 aacagcgggt caacgcccgg gaatttattg aacggcagca ccacttcttt caccgagtag
     8521 tacggcggga taacttcttt ggttacgccc tgctcagcca gcgatttgcc agccatcaca
     8581 cgcgccgcca cttttgccag cggtacgccg gtggctttgg agacgaacgg aacggtacgc
     8641 gccgcacgcg ggttaacttc aatcaggtag acttcgttgt ttttcaccgc aaactgcacg
     8701 ttcatcaggc cgcgcacctg cagttcgaag gccagtttct gcacctgctg gcgcatcaca
     8761 tcctgaattt cctgacttaa ggtgtaggct ggcagtgaac atgcggagtc accggagtgc
     8821 acacccgcct gctcaatatg ctccatgatg ccgccaatca gcaccatttc gccgtcgcag
     8881 atggcatcca cgtcaacttc tactgcgtca tcaaggaagt ggtccagcaa cactggcgca
     8941 tcgttagata cgctgaccgc tgtctggaag tagcgacgca ggtcagcttc gtcatagacg
     9001 atttccatcg cccgaccgcc gagaacgtaa gacggacgca ccaccagcgg gtagccaatc
     9061 tctttcgctt tctcaaccgc catttcgatg gtggtaacgg tggcgttcgc aggttgtttc
     9121 agtttcagac gctcaaccgc atgctggaag cgttcacggt cttccgcacg gtcgatggca
     9181 tccgggctgg taccgataac cggtacgcca gcagcttcca gcgcgcgcgc cagtttcagc
     9241 ggggtctgac cgccgtactg gacgataacg cctttcggct tctcaatacg cacaatttcc
     9301 agcacgtctt ccagcgttac cggctcgaag tagagacggt cagaggtgtc gtagtcggtg
     9361 gagacggttt ccgggttaca gttaaccata atggtttcat aaccatcttc gcgcagcgcc
     9421 agcgaggcgt gtacacagca gtagtcgaat tcgataccct gaccgatacg gttcgggccg
     9481 ccgccgagca ccatgatttt ttcacgatcg gtggacggat tcgcttcgca ctcttcttca
     9541 taagtggagt acatgtaagc ggtgtcggtg gcgaactctg ccgcacaggt atccacgcgc
     9601 ttataaaccg ggtgcaagtc atactggtcg cgcagcttgc ggatttctgc ttcacgcacg
     9661 cccgcgagtt ttgccagacg cgcatcggca aagcctttgc gtttcagctg gcgcaggaat
     9721 tcagcgttca ggccagtgat gcccacttct gcgactttct cttccagacg caccagctct
     9781 tcaatctgta ccaggaacca gcggtcaatg ttggtcaaat taaacacgcc gtctacagac
     9841 aggcccgcgc ggaaggcatc agcgatatac cagatacgct ctgcgcctgc atctttcagt
     9901 tcgcggcgga ttttagtcag cgcttccggg tcgtcgagac tcactttcgg atcgaagcca
     9961 gttgcgccca cttccaggcc gcgcagcgct ttttgcaggg attcctgctg cgtgcgacca
    10021 atcgccatca cttcgccaac cgatttcatc tgagtggtca gacggtcgtt agcaccggcg
    10081 aatttctcga agttgaagcg aggaatttta gtaaccacat agtcgatgga cggctcgaag
    10141 gaggccggag tacgcccacc ggtgatgtcg ttcatcagct cgtcgagggt gtaacccacc
    10201 gccagtttcg ccgccacttt ggcaatcggg aaaccagtgg ctttcgacgc cagcgcagag
    10261 gagcgagaca cgcgtgggtt catttcgata acaatcaggc gaccgttttt cgggttcacc
    10321 gcaaactgga cgttggaacc accggtttca acgccgattt cacgcagtac cgccatcgag
    10381 gcgttacgca tgatttgata ttctttgtcg gtcagcgttt gggctggcgc gacagtgatg
    10441 gagtcaccgg tgtggatgcc catcgcatca aagttttcga tagagcagac gatgatacag
    10501 ttgtcgtttt tatcacgcac cacttccatc tcgtactctt tccagccgat cagcgactca
    10561 tcaatcagca gctctttagt cggagaaaga tccagaccgc gggcgcaaat ttcttcaaac
    10621 tcttcgcggt tataagcgat accgccgccg ctaccgccca tggtaaagga tgggcgaata
    10681 atgcacggga agccaacttc agcggcaacc gccagcgctt cttccattga atgagcgata
    10741 ccggaacgcg cagtttccag accgattttc ttcatcgcta cgtcgaaacg gcggcggtct
    10801 tcggctttat caatcgcatc ggcagtggca ccaatcatgg tgacgccgaa ctcttccagc
    10861 acgccctgac gctcaagctc cagcgcacag ttcagcgccg tctgcccccc catggttggc
    10921 agcaccgcgt ccgggcgctc tttttcaata atcttgcgta ccacttccca gtgaatcggc
    10981 tcgatgtagg ttgcatcagc catttccggg tcggtcatga tggtcgccgg gttggagttc
    11041 accagaatga cgcggtaacc ctcttcacgc agggctttac acgcttgcgc gccagagtag
    11101 tcaaactcac acgcctgacc gataacaatc gggcccgcac ccagaatcag gatacttttt
    11161 atatctgtac gttttggcat ggctctttta ctcctgatta cttagcggtt ttacggtact
    11221 gctcaattaa cgcgataaag tggtcaaaca acggcgcggc gtcgtgtgga ccagggctgg
    11281 cttccgggtg tccctggaag ctgaacgccg gtttatcggt acgatgaatg ccctgtaacg
    11341 taccgtcgaa cagggattta tgtgtgacac gcaggtttgc aggtaatgtt gcttcatcca
    11401 ccgcaaaacc gtggttctgg gcggtgatca tcaccacatt tttctcaaca tctttaaccg
    11461 gatggttgcc gccgtggtga ccaaatttca ttttgacagt cttcgcaccg ctcgccagcg
    11521 ccagcagctg atggccgagg cagatgccga ataccggaat atcggtttcg aggaatttct
    11581 ggatggcggt aatggcgtaa tcgcacgggg ccgggtcgcc aggaccgttg gagaggaaga
    11641 tgccgtctgg attcattttc agcacatctt ccgcagaagt ttgcgccgga acgatggtca
    11701 gacgacagcc tctatccacc agcatccgca ggatgttgcg cttggcacca aaatcataag
    11761 ccacgacgtg gtacggcagc tcgtcttctt ttttcgcttc tggcagaccg ccggtcagcg
    11821 tccagctccc ttgtgtccag ctatagggtt ctgcggtagt cacttctttt gccagatcca
    11881 tgccattcag gccagggaac gcgcgggctt tttctaacgc cagcgccgca tccgggttat
    11941 cgcctgcgat aatgcagcca ttctgtgcgc ctttctcgcg cagtaaacgc gtcagcttac
    12001 gggtatcgat atcggcaatc gccacgatgt tatggcgctt caggtaagaa gagaggtctt
    12061 cggtattacg aaagttgctg gcaatcagcg gcaggtcgcg aatcaccaga ccttgggcat
    12121 gtacctgaga agattcttca tcggcgtcat tagtgccgac attgccaata tggggataag
    12181 taagagtaac gatttgacga gaataggaag gatcagtgag gatttcttga taaccggtca
    12241 ttgaagtatt gaaaacgact tccccaaccg ccgaacctgt tgcccctatg gcccgaccgt
    12301 gaaactgggt tccgtcttcc agaaccaata gcgctgactt aatcaaaaca ccctccagag
    12361 aatattcact cactttattt gcatattaat tcacaatgat gacgtaaatc aatgcaaatc
    12421 tgcttaccga cgaatttctg gcaaacggcg gcattctgga gatatgggag ctaaaagtca
    12481 acttaatggc gggatttttt atattatttt gtggctcatg catgttttac aggcattaag
    12541 cgacaaaaag atcatataac atgttctgga aaacgcttgc gcagcaagaa gattgcatga
    12601 aatcaaaatg agtagaaaaa a
//
LOCUS       DACADF010000133        12512 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.135, whole genome shotgun sequence.
ACCESSION   DACADF010000133 DACADF010000000
VERSION     DACADF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.135"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(96..869)
                     /gene="uxuR"
                     /locus_tag="ISB30_003707"
     CDS             complement(96..869)
                     /gene="uxuR"
                     /locus_tag="ISB30_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAP1991299.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1084..2544)
                     /locus_tag="ISB30_003708"
     CDS             complement(1084..2544)
                     /locus_tag="ISB30_003708"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAP1991300.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2625..3809)
                     /gene="uxuA"
                     /locus_tag="ISB30_003709"
     CDS             complement(2625..3809)
                     /gene="uxuA"
                     /locus_tag="ISB30_003709"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP1991301.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4149..5492
                     /gene="gntP"
                     /locus_tag="ISB30_003710"
     CDS             4149..5492
                     /gene="gntP"
                     /locus_tag="ISB30_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAP1991302.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5666..6568)
                     /gene="fimH"
                     /locus_tag="ISB30_003711"
     CDS             complement(5666..6568)
                     /gene="fimH"
                     /locus_tag="ISB30_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAP1991303.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSAHDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6588..7091)
                     /gene="fimG"
                     /locus_tag="ISB30_003712"
     CDS             complement(6588..7091)
                     /gene="fimG"
                     /locus_tag="ISB30_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAP1991304.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATFDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7104..7634)
                     /gene="fimF"
                     /locus_tag="ISB30_003713"
     CDS             complement(7104..7634)
                     /gene="fimF"
                     /locus_tag="ISB30_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAP1991305.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNDIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7644..10280)
                     /gene="fimD"
                     /locus_tag="ISB30_003714"
     CDS             complement(7644..10280)
                     /gene="fimD"
                     /locus_tag="ISB30_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HAP1991306.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNMASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCI
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10347..11072)
                     /gene="fimC"
                     /locus_tag="ISB30_003715"
     CDS             complement(10347..11072)
                     /gene="fimC"
                     /locus_tag="ISB30_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAP1991307.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11109..11648)
                     /locus_tag="ISB30_003716"
     CDS             complement(11109..11648)
                     /locus_tag="ISB30_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP1991308.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11713..12261)
                     /gene="fimA"
                     /locus_tag="ISB30_003717"
     CDS             complement(11713..12261)
                     /gene="fimA"
                     /locus_tag="ISB30_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/fibrin-binding adhesin FimA"
                     /protein_id="HAP1991309.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         2955 a         3289 c         3058 g         3210 t
ORIGIN      
        1 aaacaatccc ctgcaattta caaagtaata ctcattgcga cttcttaatt taagaaagcg
       61 caaggaacgt ttacccttgc gcttattata ataagtcagg cgtcgacttt atccagcggc
      121 catgaatcaa acagatagcc atcaaaataa atatcgtcaa cgttcgagaa ttccagcaga
      181 cgttgcttaa cgttttccag atgctgccac attgccagct tcgccgctcg ggcatctttt
      241 ttgattaacg cggcgaggat ctgtttgtga tcgcccaacc actctttgcg atacaggctg
      301 tcatccagat ggctgtgcaa ctggatccac attggattgt tttcccgcca ctgccaggac
      361 tgacggaaca gctccaccag catgctgtta tgcgttgctt cggcaatagc gagatggaac
      421 tgcatgtcac cgctttcgct gctgcccggc gcactggaag ccagttcacg ctcttccagt
      481 tgcaatgcct gacgcatttt gacgatatct tcgcgggtag cctgcagggc ggcaaactcg
      541 gcgatgttgc tctcaagaag ctgtcgcgcc tgcaacagct caaaaggacc cgcatcattg
      601 caaacgttgg catccggact gtctgtgttg tggctgcctg agctatcaag aacatagata
      661 ccggcacccc ggcgcacttc caccagccct ttgatctcca gcatgatcag cgcttcacgt
      721 accaccgtcc gcgtgacatc aagcatttct gcaatttcac gctccggcgg caaccgttcg
      781 ccaggattgt acggcgtctt tatgatcaga tcgcggatca tcgccccgac ttcctggtaa
      841 ggtctttgcg cagaggtggc agatttcatg gtgttgtgtc cgttaggtgc ggttaatcaa
      901 atttttgccg gatgcggcgt gaacgcctta tccggcctac aacgggcaat tttacggcac
      961 ccgttaatct caaactatac cattagcact ggaagttggc acgtacaggt gggcaaaaaa
     1021 acgaaaactg gtcaaccaaa tatcagtttg attgaccagt aagtctgtag ggtaatcagt
     1081 tagttacagc gcagccacac actcgcgcgc accgcgttcg cacagctgct gatatgccgc
     1141 cgtcaccgcg ccaacaaagt cggcattctg cggcagatca tcggcaaaaa taccgctcag
     1201 gcccagcagc gctttcacgc ggtctgcgcc ctgatactgc gcgttaatct tctggaactc
     1261 ggccagcatc gggtcgacca cgtcaatggc attaccctgc tcatccacgc cctgggtgta
     1321 acgcatccag ccagccacgc ccagcgccag gtgacgccag ctgccgccgt tttgcaggtg
     1381 cagacgcacc ggatccagca gacgctgcgg taacttctgg ctgccgtcca tcgcaatctg
     1441 ccaggtacgg tgacgcagag acgggttgct gaaacgctcg atcagcagcg tcgcataggc
     1501 gttcaggtcc gtaccttccg gcatcgacag cgttggcgct tgttcctgca tcatcagggc
     1561 aaaggccgct ttgcgataat ccgggttagt catggtgtcg gcaatagttt catagccgcc
     1621 gaggtaaccg aggtatgcca ggaaagagtg gctgccgttc agcatacgca gcttcatcat
     1681 ttcgaacggc acaacgtctg caacgaactg tgcgcctact ttatcccagt ccggacgacc
     1741 attaacgaag ttatcttcaa tcacccactg acggaacggt tcgcaggcaa tggcgcacgg
     1801 gtcataaaca cccagttggt cagcaatttc ctgtaaggtt tctggcgtcg ccgccggaac
     1861 gatgcggtca accatggtgc acgggaaggt gacgttctct tcaatccatg ccgccagctg
     1921 cgggtcacgc gcctgagcca gccccagtac cgcgaccttc gccacatgac cgttttcacg
     1981 cacgttatcg caggacatca ccgtaaacgc tttcagccct ttttcacgac gcagacgcag
     2041 ggcttcgacg atgtaaccaa tcgcggactt cggcgcagtc gggttttcca gatcgtgctt
     2101 gatcagcggg ttattgagat ccagttgacc gcttgccgca tcagcgcagt agcctttttc
     2161 cgtgaccgtc agagagacaa tcgccgtttg cggacgcgcc atcgcgttga gaataccttc
     2221 gcagccgtcg atttccgggt gcagcgcttc tttcatcgaa ccgataattt tcagctcggt
     2281 gctttccgcg cctttttccg ccacggtgta cagcagttgc tgttttttca ggttttcgat
     2341 cagcacgcgg tcgttgcctg gcatcaggtt aacttcgcag ataccccagt cgctgtcggt
     2401 gctttccagc agatgatggg tgtacagcgc ctggtgcgca cggtgaaacg ccccgcaacc
     2461 gagatgcaca atgcgtgatt ccagacgaga atgatcccat gacgggcggg caaccggcag
     2521 attgctgtca acaatagtag tcatcgtaaa ctccagaatc cgtcttcaga gagtgacggc
     2581 aaggaagaag gaaccggcac ggcgcgcagc catgccggtg gatattaacg gctaaagaaa
     2641 gcgcgctgga tcgccagttc gacaccgcga acttcggcca ggcctttcag acgaccaatt
     2701 gcggagtaac ctgggttggt tttcttcttc aggtcgtcca gcatctgatg accgtggtcc
     2761 ggacgcatcg ggatcaggtc ttctttgcct tccgctttac gacggtgttc ttcttcaaca
     2821 atcgctttca ccacttcgta catatcaacg tcaccgttca ggtgcgccgc ttcgtggaag
     2881 gttttcgggt tatcttcacg catggtggag cgcagatggg tgaagtaaat acgcggaccg
     2941 aactgcttga tcatatcaac cagatcgttg tcagcacgca cgccgtagga accggtgcac
     3001 atggtgaaac cgttcgccat gctatttacg gtatcaacca tccactgcat atcttcaatg
     3061 gtagaaacaa tgcgcggcag gccgaggatt gggcgcggcg gatcgtccgg gtgaacagcc
     3121 atacgcacgc caacttcttc ggcaaccgga ataatcgcct tcaggaagac ggcaaagttt
     3181 tcgcgcagtt tggctttgtc gatatctttg tacagctcca ggtgcttacg gaactggtcg
     3241 agggtgtacc cttcttccgc gcccggcaga ccggcaatga tgttacgggt cagacgcgct
     3301 ttgtcttcgt cgctcatagt ggcgaagcgt tcagctgcct gagcaatttc ttcttcggtg
     3361 taatccgctt ccgcacctgg gcgtttcagg atatgcattt cgaatgcagc gaattcgatc
     3421 tggtcgaagc gcagagcttt ggagccgtct ggcagcacgt attcgaggtc agtacgggtc
     3481 cagtcgagca ccggcatgaa gttgtagcac acggtgcgaa taccgcactg cgccaggttg
     3541 cgcagagtct gctgatagtt agcgatccac tgctcatagt tgccagtgtg ggttttgata
     3601 tcttcgtgaa ttggcacgct ttctacgaca gaccacacca ggcctgcatc ttcaacgata
     3661 gccttgcgtt tgaggatctc ttcaacggac catacttcgc cgttcgggat atggtgcagc
     3721 gcggtaacca cgccagttgc gcccgcctga cggacatcag ctaaagaaac cggatcgttt
     3781 gggccgtacc agcgccaggt ctgttccatg atttttcctc tttcacatag tgggttccac
     3841 agcaaagaga cgcggaaccc tggagttgtt taggtatccg gatgaacaac cagaccgcag
     3901 ccgtggcgtt gcatcggatg agatggcgta taagttctac cttttggtaa tacatgtcaa
     3961 cccgaattct aaaattggtt aaccacatca caagaatttc actctcctgg ttgttgctgc
     4021 aaattgcagc aaagggtaat cgatggttat tgatcagagg atgtgacatt catcgcaaca
     4081 atggttgacc aatttacata acatgtcgcc caaaataaca acggttcaac caggtaaggt
     4141 aaaacgttat gcatgtgctt aacattctct gggtggtatt cggcattggt ctgatgctgg
     4201 tactgaattt gaagttcaaa atcaattcaa tggtggcttt gttggtggcg gcgctgtccg
     4261 tcgggatgct ggcgggcatg gatttgatgt cgctgctgca caccatgaaa gcgggcttcg
     4321 gcaacacgct gggggaactg gctatcatcg tggtgttcgg tgcggtcatc ggtaaattga
     4381 tggtcgactc cggcgcggct caccagatag cacatacgct gctggcgcgt ctcggtctgc
     4441 gctatgtaca gctgtcggtg attatcatcg gcctgatttt tggtctggcg atgttctatg
     4501 aagtggcctt tatcatgtta gcgccgctgg ttattgtcat tgccgccgaa gctaaaattc
     4561 cgttcctgaa actggcgatc ccggcagtag cagctgccac taccgcacat tcactgttcc
     4621 caccgcagcc gggtcctgtg gcgctggtga atgcttatgg cgcagatatg gggatggttt
     4681 atatctatgg cgtactggtg acgatcccaa gtgtaatctg cgcaggtctg atcctgccga
     4741 agttcctcgg caatcttgag cgcccaacgc catcattcct gaaagcagat caaccggtag
     4801 atatgaacaa tctgccctct ttcggcgttt cgattctggt gccgctgatc ccggcgatca
     4861 ttatgatctc taccaccatc gccaatatct ggctggtaaa agatacccct gcctgggaag
     4921 tggttaactt tatcggttcc tcgccgattg caatgtttat tgcgatggtg gttgcattcg
     4981 tactctttgg caccgcacgt ggtcatgaca tgcagtgggt gatgaacgct tttgaaagtg
     5041 cggtgaaaag cattgcaatg gtgattctga tcatcggtgc gggtggcgtg ttgaagcaaa
     5101 ctatcatcga caccggcatt ggcgacacca tcggcatgtt gatgtcccac ggcaatatct
     5161 cgccctacat tatggcatgg ctgatcactg tgctaattcg tctggcgacg ggtcagggtg
     5221 tcgtttcggc gatgaccgcc gccgggatta tcagtgctgc aatccttgat ccagcaactg
     5281 gtcagctggt tggcgtgaat ccggcgctgc tggtactggc gacggctgcg ggttccaaca
     5341 ccctcaccca cattaacgat gcatctttct ggttgttcaa aggttacttt gacctgtcag
     5401 taaaagacac gttgaaaacc tggggtctgc tggagctggt caactccgtg gttgggctga
     5461 ttattgtgtt gattatcagc atggtagcgt aaaagagcac ctgagcctgc cattggcagg
     5521 ctctgttcgg attgtcggca aagtgcactt tgtttatgcc agatgcgacg ctggcgcgtc
     5581 ttatctggcc tacaaagggc taacgtgcag ttttttagct tcaggcaata ttgcgtacct
     5641 gcattagcaa tgttctgtga tttctttatt gataaacaaa agtcacgcca ataatcgatt
     5701 gcacattccc tgcagtcacc tgccctccgg tacgtgcgta atttgccgtt aatcccagac
     5761 ttaccgccga agtccctact gctcctaacg ataccgtgtt attcgctgga ataatcgtac
     5821 cgttgcgcgt caactgtacg ccgacgccct gcgctggtga aaacgacgcg gtattggtga
     5881 aaatcgagtt gcccgcatct gcggttgtgc cggagaggta ataccccagg ttttggcttt
     5941 tcgcacaata aacggtaaga ggaattggca ctgaaccagg gtagtccggc agagtaacgg
     6001 tgacatcatg agcagaaaca tcgcagccgc cagtaggcac caccacatca ttattggcgt
     6061 aaatattcca cacaaactgg aaatcatcgc tgttatagtt gttggtctgt cgcaaaataa
     6121 gcacggcaat taatgagcca gctttaatcg ccaccccacc cgcactgctc acaggcgtca
     6181 aataaagcgc caccggccac ggcttatccg ttctcgaatt ataaacaacc cgcggcgttt
     6241 cgctggtagt cggaaatgga tagctactgc cactatattt tacggtcccg gaaaaattag
     6301 ataacacgcc gccataagcc gagcctcgtt gcagtgtgac atagtctgta atggtttccg
     6361 gataatcgtt atggcaaaag atttgcgtcg aaagatctac gaccaggttt tgccccacat
     6421 tcacggcagg cgcaaggttt acataaacat tagcgctgcc accgccaata ggaatagcgg
     6481 taccattggc ggttttacag gcgaatgacc aggcatttac cgaccagccc atcagcagta
     6541 cagcaaacag ggtaataact cgtttcatga caattatctc ttcgggttca gctgtaggta
     6601 taggtgatgc taatcactgc ctgaatggtt ccctgagtgg ctccgccatt taccgtcaat
     6661 gctctgacct gtaacgggaa gtgcgctgat tgtgaggaat catccacctg aactgttttg
     6721 gttgcgccag tattcaatgt gttgccactg tcatcctgta gctctaactg gatgttttgc
     6781 gcggtcccct ggtttttata atatccggta ctgtcggctg ccccgctgaa gctggcagtg
     6841 accctcgacg ttcccaccgg acaattagtc aactcaagcg caacatcatg ccaggccgat
     6901 gccgccccgg cagacatcag actgaaagaa taaagatcgc cgagatcaaa cgtggcattg
     6961 gtggtggaaa ccgtgcacgg tttggcgacg accttaccgt tcaccgtgat ggtgacatcg
     7021 gctgcctgta tcgtcgcact tgcgagcgcc aatattgccg ccaataaata cccacgtttg
     7081 cgccatttca tgagcatctc cagttactga tattcaagag tgaaggtagc cgtggcattg
     7141 atatgccccg cagtgacagg cacctgtgtc gccattagcc gggcgtaaaa attcagcgta
     7201 tttggtttac ccggcgtcag ggtcgtccac gaaagcgcgg acgatggagc attaaggggt
     7261 atttgatttt gctgctcatt cagaagctgt attcccagtc ccgaagccgc tgacaccgta
     7321 ttttcaagtg caagcaggtt ggcattgtgg ctatctgcaa caccggtaaa cccaactttt
     7381 acggcagaaa cggcattacc acagggtgac agcaaaatac gaaatggaac gacaggagtc
     7441 gtcgcgccaa tgtcgttaaa ttgcttcgcc gcgttttcca tcaaatcaac agtaaaattg
     7501 gttgattcag cggccacact acagccgtta tctctgacat agccgcggat agtaatcgtg
     7561 ctatccgcag ccaaaacgcg acttaccgcc agccacaaaa aagcgcacag aagataaaaa
     7621 ggtttgtttc tcatcacgcc cccttaacga cattcagctg atagctgggt taataactgc
     7681 tgctgactct ctggtggcag ttgataattg gcgatacagt gagcattttc ctcttctccc
     7741 catttcacct gaacttttcc cgctaaaggc attccgctga ggtaaacctg accattatcc
     7801 gcaacaatgc cgctactctg gctactctct gatgtcacca tcgccccaaa cggcagcggc
     7861 ttattattgt gggttagcgt catgagcagt tttatcccaa cgcgcgcttt aaactctgct
     7921 cgcacgatcg ccccacgagt gggaacaacg ttagcgaccg cgttatctaa atcgacgtta
     7981 tcagccaggg tattggtatc cagcgccact ctgttttccc gatattcggt ggcataaggc
     8041 agcacggcat aaccgcgcca gtcggtacgc acccccgtct ggttttcgac ttttgcatct
     8101 tttgcgccag gcgctttaac aagtaccacc gtatcgttta acggctgccc cagcgttacg
     8161 ccattggcat gagccagtac cccaccgctg actccgtaat agagctgctt aatatcatcg
     8221 ctatggctgt aaccgatatt ggcattgccg taaccaccgc gataattcag cgtggcgtag
     8281 cctgtacttc cgctatttcc atcgcctccc ccggcatagc cggtttgcac gctatagctg
     8341 aggttgttgt cttccagcaa cgtaccgtat acaccagcca gattggtcat ccgaccgttg
     8401 agatcgtgtg acatgctgta gctggcactg gcatgtcgcc actgagattt actgtcagaa
     8461 cgcagccagt ggctgaaagg aatattgacg ttaagcgcta acatctgatc ccgtcctttt
     8521 tgccaggcgt ttttcgtcag gctatagctg agcgtccagt tgatatcttc gaacgcagta
     8581 tttaatccag cctggaattg ctcatcgaca ttactcgttc cccaataagt ttgatggcta
     8641 ccactcaaat acagtgttga tgagcgcccg agttgctgag taacggtgag ttgtaatttc
     8701 ccgcgtttgt tataagcgag gttgtaatag tcggtgaatt tcggcttaac ctgaataaca
     8761 ccatcctgtg tttcgatgtt gtagccattc attcgactgt atgttgtatc agcgaaatta
     8821 aaatatccgc tggtcgaata acggtaaccc actaactgaa tattcgtgcc tgactcattg
     8881 agcgatttgt tatagagaaa acgcaccgat tgtccgtcat gctgactgtc atcgggaagt
     8941 gtggaattag cctgcgtcat atccacagac agagcaccca gcgcccccat gtttttcccg
     9001 ataccgaaat taaaagcacg ataacgatcc gccagttgcg ttccaccata tattgtccag
     9061 ccagccggaa ggccgtggag taatgtactc tggaaaaagc ggggtttttc ctgttgcgca
     9121 tttccactac ggtattctcc tgccgtaatg gaataacgag tatgcccttc acgttgcaaa
     9181 agcgggactg acgaataggg tacggtaaaa atctgcgtgc tgccgtcagc ctctttgatc
     9241 gttacctgca agtcaccact attacctgcg gcatagatat cgttgatggt aaaaggcccc
     9301 ggtggcaccg tactattata aatgtcatac ccattttgtt taatagtgac ctgtgcagta
     9361 ccacgagcaa taccgtggat caccggggca aatcctcttt gactatcggg taacatattg
     9421 tcatctgagg ccaattgtgc gccgcgaaag ttaataccat cgaaaatatc gccctgagta
     9481 taaccatcac ccagcgtcag ccgggaacgt aacggtatta tgtctcgctc aagccaggta
     9541 ttgatatgct gccatttatt tttgctacct gatgatctgt cgctactgtt ataactccag
     9601 gtggtattgt cgcgtaaacg ccacgcacca atatttaacc cactctgtag gtttaaatat
     9661 gcataatggc tgttaccccc aatccgattc tgtacactat ttccgctgaa attataattg
     9721 agcaatccgg cattaatacc gggatcccat aactcaggag gaatataacc acgcgcgcga
     9781 ttactcataa atgcctgagg gatcgtcagg ttgagtcgct gctgaccaac atctaaatgc
     9841 gcagtagcgt cctggaccat tgtggttaat ggcacacagg catcatccgc cagcagattc
     9901 ataccggcga cagaagccat attcagcccc atactggcga gttgcgcgcg tgtcaggcag
     9961 ggaacaatcc cttgttcact gtcgcccgta ttaaatgtga catcacgcgt tgccatataa
    10021 ccattattca aatagatatc gacgcgatac gtccctggcg gtaattcttg tccattttca
    10081 aaacgcgata aatcagccac agcctgggga tcatccgcta aaaagcgcgg gttaaaatag
    10141 agttcggcag atgacaaagg tgcctgtgcg gcaaaagcac aggcaacaaa gagccggaca
    10201 aaaaaaccag ccaaacgatg cttacgaata tgcaagcatt gtgtgtttcg ctggtaaagt
    10261 cttaaattca gatatgacat tattcctccg gtttaaaatc acccggcagt caattctttt
    10321 tattcaggcg aaaaattccc cctgcgttat tccattacgc ccgtcatttt gggggtaagt
    10381 gcgccataat catttattgt tcggtaagta atattgcttc ctgcatcaga aggcaattta
    10441 accgtgcttt cgcccattgg aggcaccaat gcattttcaa gaacccgggt tccggcattc
    10501 aactctgtta ccgtcaggta atagggtgtc gggttaatca gcgtcagaga attcgcgcta
    10561 cgacgaaatc ttaatttttc tgcggcctga tcgggtggca acgctaattt agccggacga
    10621 tagtacagtt taatgcggct gataattgcg agctgtagcg tattctcagt caattttgat
    10681 ttatccattg acggaatcgc tttaacgttc atccagaata aactttcccg gtcctgtggc
    10741 aattggttat ttgttgcatc aagaatacgc aaggtattct ctttttttcc cttcatcgca
    10801 aacagaggag gcgtcacgat aaaacgacca tcctttacac catcggcatt ttccacccat
    10861 gattgaatta aataggtgct attttcatca ttatttgtca cggcaagttg cacttgtttt
    10921 tgccctgccg gataaattac gcgagtcgca cctaaggcca ctcccgcttc agcgcgtccg
    10981 gcaaccatca ttgccatgaa catcaggata cctgccagca agcagaatgt tatttcctgc
    11041 gatttcctta cattgacgtt tttattactc actgtcctgt tcctgttatc acattatctg
    11101 ctgaacaatt actgataggt taaagagaac caggcctgtg cattggcgat gccgccagta
    11161 acccgacgcc cggtagcacg atatttggcg atgaaatgta gcgaagtaga gcctgagtaa
    11221 agccgtttcc agtttgctgg aggacgatta atcggtacga ggtttccttc atcatcaaac
    11281 aacgctacgc caatattggt ggctatccct ggcccctctc ccacggaaag cacatccgga
    11341 tttttaccat ccgcgacacc gtgaaacgcc acacccacac gttcactcac caccgtgcta
    11401 cattcccgta aatgaataac aaaaggcacc ggtgcgctat cttcccctac cgcgtgaaac
    11461 cggttactgc tgatttgccc catattgacc gtcatttgtt tatcaccagc ttcaatccgg
    11521 caagtttccg caataatgac gccctgaaat tgcatatttc cgccgggcag cgtggtattc
    11581 catttatttc cggccagagc aaacattggc agcaacgacg ctaatagaaa taatctcttc
    11641 cttttcatcg ttatttttat cacacaaggg tgggcatccc tgcccgtaat gacgtccctg
    11701 aacctgggta ggttattgat actgaacctt gaaggtcgca tccgcattag cagcacccgg
    11761 ggttgcggca ccggttgcaa aataacgcgc ctggaacgga atggtgttgg ttccgttatt
    11821 cagggttgtt tctgaactaa atgtcgcacc gtccagcgcc agcgcagcac ccgttctgtc
    11881 caggatctgc acaccaacgt ttgttgcgct acccgcagct gaactctgca gagccagtac
    11941 atcagtatgc gtagcatcaa ttgccgtacc taagaaggca acagcggctt ttgtggcaac
    12001 agtggtatcg caatcattca gctgaatgtt aaaaccaacg gcagagctgg ttgctccagc
    12061 ctgcttcagg ctagcggtac gaacctggcc taactgaacg gtttgatcaa cagagcctgc
    12121 atcaactgcg caagcggcgt taacaacttc ccctttaaag tgaacggtcc caccatttac
    12181 cgtcgtagta tcggccagag ccgctgcgga actgagggac agagccgaca gaacaacgat
    12241 tgccagagtt ttaattttca tgctgctttc ctttaaaaaa ctatttctaa atcgacatgg
    12301 gcagtcgttc tgtacacttt gttttgtcaa cgagtttgac tgccaacact gcacagtttt
    12361 cccccaaaag atgaaacatt tggggccatt ttgactcata gaggaaagca tcgcggacaa
    12421 actttttcag tttatttgtt ggcttaatat tctattgtta tctttattta tagatgttta
    12481 tattgcatga ggtggttttt ggagagaaga at
//
LOCUS       DACADF010000134        12473 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.136, whole genome shotgun sequence.
ACCESSION   DACADF010000134 DACADF010000000
VERSION     DACADF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.136"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(84..635)
                     /gene="ybcL"
                     /locus_tag="ISB30_003718"
     CDS             complement(84..635)
                     /gene="ybcL"
                     /locus_tag="ISB30_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAP1991310.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(804..1136)
                     /gene="emrE"
                     /locus_tag="ISB30_003719"
     CDS             complement(804..1136)
                     /gene="emrE"
                     /locus_tag="ISB30_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAP1991311.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1480..1794)
                     /gene="fliE"
                     /locus_tag="ISB30_003720"
     CDS             complement(1480..1794)
                     /gene="fliE"
                     /locus_tag="ISB30_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAP1991312.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2009..3667
                     /gene="fliF"
                     /locus_tag="ISB30_003721"
     CDS             2009..3667
                     /gene="fliF"
                     /locus_tag="ISB30_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HAP1991313.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQALAREEMEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3660..4655
                     /gene="fliG"
                     /locus_tag="ISB30_003722"
     CDS             3660..4655
                     /gene="fliG"
                     /locus_tag="ISB30_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAP1991314.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4648..5334
                     /gene="fliH"
                     /locus_tag="ISB30_003723"
     CDS             4648..5334
                     /gene="fliH"
                     /locus_tag="ISB30_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAP1991315.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5334..6707
                     /gene="fliI"
                     /locus_tag="ISB30_003724"
     CDS             5334..6707
                     /gene="fliI"
                     /locus_tag="ISB30_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAP1991316.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSKTHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6726..7169
                     /gene="fliJ"
                     /locus_tag="ISB30_003725"
     CDS             6726..7169
                     /gene="fliJ"
                     /locus_tag="ISB30_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAP1991317.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7166..8293
                     /gene="fliK"
                     /locus_tag="ISB30_003726"
     CDS             7166..8293
                     /gene="fliK"
                     /locus_tag="ISB30_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAP1991318.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLTGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8398..8862
                     /gene="fliL"
                     /locus_tag="ISB30_003727"
     CDS             8398..8862
                     /gene="fliL"
                     /locus_tag="ISB30_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAP1991319.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8867..9871
                     /gene="fliM"
                     /locus_tag="ISB30_003728"
     CDS             8867..9871
                     /gene="fliM"
                     /locus_tag="ISB30_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAP1991320.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9868..10281
                     /gene="fliN"
                     /locus_tag="ISB30_003729"
     CDS             9868..10281
                     /gene="fliN"
                     /locus_tag="ISB30_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAP1991321.1"
                     /translation="MSDMNNPADDNNGAMDDMWAEALSEQKSTSGKSAADAMFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10284..10649
                     /gene="fliO"
                     /locus_tag="ISB30_003730"
     CDS             10284..10649
                     /gene="fliO"
                     /locus_tag="ISB30_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAP1991322.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10649..11386
                     /gene="fliP"
                     /locus_tag="ISB30_003731"
     CDS             10649..11386
                     /gene="fliP"
                     /locus_tag="ISB30_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAP1991323.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11396..11665
                     /gene="fliQ"
                     /locus_tag="ISB30_003732"
     CDS             11396..11665
                     /gene="fliQ"
                     /locus_tag="ISB30_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAP1991324.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11674..12459
                     /gene="fliR"
                     /locus_tag="ISB30_003733"
     CDS             11674..12459
                     /gene="fliR"
                     /locus_tag="ISB30_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HAP1991325.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
BASE COUNT         3039 a         3277 c         3309 g         2848 t
ORIGIN      
        1 accctgtgaa tctcatgatg tttatgcatc cgtatttctt caatctttag gttagtctta
       61 tcttttgcca gcatactctt accctacttt atctcataaa ctggtgttat ctcagcggtt
      121 gcaattttat tggcattaag catataacca actaacgctc cgctggaatt agaatccaca
      181 ggaattttat cagtttttag agcccatact ttaaactggt aatgatgggg tttatctcct
      241 ttgggaggac atgctccacc aaatcctgca taaccaaaat catttcggcc ttgaacagca
      301 ccagtcggaa gttttgtacc atcacgtctt cctgcatcag cgggcaaata tgttacagtt
      361 gctggaatat tagcaacagt ccagtgccac catccactgc ctgtaggagc atctggatca
      421 tacacagtta cggcaaagct tttggtgcct tcaggagcac cagaccaggt taatgagggc
      481 gatgtattac caccttcaca cccaaatcca gaaaatacat gagacgttgt aagttgctct
      541 cctgttttta tttcattact agtgacctga aatgctgcgg cctgcgcaga aaatgttatg
      601 aatgccaata cacttgaaac gataagtttt ttcataaaaa cctcattgtt gtgacctatc
      661 gttattttat ttgatgtgcc ttgatctcat tatgcataaa ggcgcaatga tcgcgcaaat
      721 gcaatcataa ttataaaggt gccccgaaca ttagccataa tatgcacaaa tcagattcct
      781 gttatctcaa ggataattat tttttaatgc ggtgcacttc gtgacaacaa gttaatgact
      841 aacacaccgg cacaaatcaa catcatgcct atgatagctg gcaggtccag tcgttgacca
      901 aaaaatcccc aggaaagcaa actaatcaga acgataccga ctcctgacca gatagcataa
      961 gcaatccctg taggtatata agccagcgtc tgagctaata accagaatga tgcacaatag
     1021 caaataattg tgccaacaga gggccataac cgtgtaaacc cctctgaaaa cttcattaag
     1081 gttgtaccaa tgacctctgc aagtattgcg ccaccaagat aaatataagg gttcatagca
     1141 tattctttcc tgttcatact ggagagaatt gtactacagt ttgaacacaa ctcactgttt
     1201 tcatcctggt gtatgtgtat ccagggggct ctggcgtggc aagagataaa ggcaggaaca
     1261 ataatgatca gaaggaaccc caggctaacg aaagagttca ctcttcctgg gagcaaagcg
     1321 aagacaaaac atgttatcgc gtttacggat cgtggatggc agaaaacaac caggatcagg
     1381 tatccatttt aaaccagaaa ttgagtgagt ttgcccatcc atgccccacg ctgtgggatc
     1441 ggatataatt aaacaagcat agtcaacagg ttacacaacc tacacctgca tggacatcac
     1501 ttcctgatac gccgccacca gcttattacg cacctgaatc cccatttgca tagaaactga
     1561 ggctttttgc atatcggtca tcacatcgtt tagcgccacg ccgggttcac cgagggtgaa
     1621 tttttctgcc tgcgtgcggg cggctgtttg tgtatcgctt atgcgatcga gcgcggcgtg
     1681 cagctgcccg gcaaaactaa tggtcggttg cggcagtgat tcctgcgcac gcgcactcat
     1741 cgccgtagcc tgtaactggc tgataacccc ttcaatcccc tgtatcgctg acattttcgt
     1801 ctcccggata atttctgata gcaaagccta ccagtaagtc aataagacaa aggcgctaaa
     1861 tagcaacaaa aaaacgggtt tattggcgga tagaaaaaaa cgaaagcaca aataatggga
     1921 gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt gttccacttt gccaataacg
     1981 ccgtccataa tcagccacga ggtgcgcgat gaatgcgact gcagcccaga caaaatctct
     2041 tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgttg ccggttccgc
     2101 agcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact accgcacatt
     2161 attcagcaat ctttccgatc aggatggtgg cgcaattgtc agccaactga cgcaaatgaa
     2221 tattccttac cgcttcagcg aagccagcgg cgctattgag gttccggcag ataaagttca
     2281 cgaactgcgc ctgcgcctgg cgcaacaagg tttgcccaaa ggcggcgcgg tcggtttcga
     2341 actgctcgat caggaaaagt ttggtatcag ccagttcagc gaacaggtga attatcagcg
     2401 ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa aaggggcgcg
     2461 cgtacatctg gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat ccccttctgc
     2521 atcggtgacg gtaaatctgt tacccggccg cgcactcgat gaagggcaaa ttagcgctat
     2581 tgtacatctg gtttccagcg cggttgctgg tctgccgcca ggaaacgtca cgctggtgga
     2641 tcagggcggg catctgttaa cccagtccaa taccagcggg cgcgatctta atgacgctca
     2701 gttgaaatat gccagcgatg tcgaaggccg tattcagcgg cgtattgaag cgatcctgtc
     2761 gcctattgtt ggtaacggta atattcacgc ccaggtcacg gcgcaactgg acttcgccag
     2821 taaagaacaa acggaagaac agtatcgccc taacggtgat gaatctcatg cggcgcttcg
     2881 ttcacgccag cttaatgaga gcgagcaaag cggttccggt tatccgggcg gcgtaccggg
     2941 ggcgttgtcg aatcaaccgg cacctgcgaa taacgcacca atcagcacgc ctccggcaaa
     3001 tcaaaataac cgccagcagc aggcgagcac caccagcaat agtgggccgc gtagcacaca
     3061 gcggaatgaa accagtaact acgaagtcga tcgcaccatt cgtcatacca aaatgaacgt
     3121 gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat tacaaaacct tgccagacgg
     3181 taaaccattg cctctcagca acgaacagat gaagcaaatt gaagctctga cccgcgaggc
     3241 gatgggcttt tctgaaaaac gcggtgattc gctcaatgtt gttaactcgc cgttcaatag
     3301 cagtgacgaa agcggcggag cactgccgtt ctggcaacag caagtgttta tcgatcagtt
     3361 gctggctgcc ggtcgctggt tgctggtact gctggtggcg tggttgctgt ggcggaaagc
     3421 ggtacgcccg cagctaacac gtcgcgccga ggcggtgaag actgtgcagc aacaagcgct
     3481 ggcccgcgag gaaatggaag atgcggtgga agtccgtctg agcaaagacg aacaactcca
     3541 acaacggcgc gctaaccaac gactgggggc agaagtcatg agccagcgta tccgtgaaat
     3601 gtctgataac gatccgcgcg tggtggcgct ggtcattcgc cagtggataa ataacgatca
     3661 tgagtaacct gacaggcacc gataaaagcg tcatcctgct gatgaccatt ggcgaagacc
     3721 gggcggcaga ggtgttcaag cacctctccc agcgcgaagt gcaaaccctt agcgctgcaa
     3781 tggcgaacgt cacgcagatc tccaacaagc agctaaccga tgtgctggcg gagtttgagc
     3841 aagaagctga acagtttgcc gcactgaata tcaacgccaa cgattatctg cgttcggtat
     3901 tggtcaaagc tctgggtgaa gaacgtgccg ccagcctgct ggaagatatt ctcgaaaccc
     3961 gcgataccgc cagcggtatt gaaacgctca actttatgga gccgcagagc gccgccgatc
     4021 tgattcgcga tgagcatccg caaattatcg ccaccattct ggtgcatctg aagcgcgccc
     4081 aggccgccga tattctggcg ttgttcgatg aacgtctgcg ccacgacgtg atgttgcgta
     4141 tcgccacctt tggcggcgtg cagccagccg cgctggcgga actgaccgaa gtactgaatg
     4201 gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg cggcgtgaga acggcagccg
     4261 aaattatcaa cctgatgaaa acgcagcagg aagaagccgt tattaccgcc gtgcgtgaat
     4321 tcgacggcga gctggcgcag aaaatcatcg acgagatgtt cctgttcgag aatctggtgg
     4381 atgtcgatga tcgcagcatt cagcgtctgt tgcaggaagt ggattccgaa tcgctgctga
     4441 tcgcgctgaa aggggccgag cagccactgc gcgagaaatt cttgcgcaat atgtcgcagc
     4501 gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg tccggtgcgt ctgtcgcagg
     4561 tggaaaacga acagaaagcg attctgctga ttgtgcgccg tcttgccgaa actggcgaga
     4621 tggtgattgg cagcggcgag gatacctatg tctgataatc tgccgtggaa aacctggacg
     4681 ccggacgatc tcgcgccacc accggcagag tttgtgccaa tggtcgagtc ggaagaaacc
     4741 attattgaag aggccgaacc cagccttgag cagcaactgg cgcaactgca aatgcaggcc
     4801 cacgagcagg gttatcaggc agggattgcc gaaggtcgcc agcaaggtca tgaacagggc
     4861 tatcaggaag gactggccca ggggctggaa caaggtctgg cagaggcgaa ggctcaacaa
     4921 gcgccaattc atgcccggat gcagcaactg gtcagcgaat ttcaaactac ccttgatgcg
     4981 cttgatagtg tgatagcttc gcgcctgatg cagatggcgc tggaggcagc acgtcaggtc
     5041 ataggtcaga cgccaacggt ggataactcg gcactgatca aacagatcca acagttgttg
     5101 cagcaagaac cgttattcag cggtaaacca cagctgcgcg tacacccgga tgatctgcaa
     5161 cgtgttgatg atatgctcgg cgcaacttta agtttgcatg gctggcgctt gcggggcgat
     5221 cccaccctcc atcctggcgg ttgcaaagtc tccgccgatg aaggcgatct cgacgccagt
     5281 gtcgccactc gctggcaaga actctgccgt ctggcagcac caggagtggt gtaatgacca
     5341 cgcgcctgac tcgctggcta accacgctag ataactttga agccaaaatg gcgcagttgc
     5401 ctgcggtacg tcgctacggg cgattaaccc gcgctaccgg gctggtgctg gaagccaccg
     5461 gattacaatt gccgctcggc gcaacctgtg tcattgagcg ccagaacggc agcaaaacgc
     5521 acgaagtaga aagcgaagtc gtcggcttta acggtcaacg gctgttttta atgccgctgg
     5581 aggaagtgga aggtgtcctg cccggcgcgc gtgtatatgc caaaaacatt tcggcagaag
     5641 ggctgcaaag cggcaagcag ttgccgctcg gtccggcgtt attaggtcgt gttctggacg
     5701 gcagcggtaa accgctcgat ggcctcccct cccccgatac gacggaaacc ggtgcgctga
     5761 ttaccccgcc atttaaccca ttgcaacgta caccgattga acatgtgctg gacaccggcg
     5821 tgcgcccaat caatgccctg cttaccgtcg ggcgcgggca gcgtatgggg ctgtttgccg
     5881 ggtccggcgt tggtaaaagt gtgctgttgg ggatgatggc acgttacacc cgcgccgatg
     5941 tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt aaaagatttt attgagaata
     6001 tcctcggtgc cgaaggacgt gcacgctcag tggtgattgc cgctccggcg gatgtttctc
     6061 cgctcctgcg aatgcaaggt gccgcctatg ccacgcgcat tgccgaagat tttcgcgatc
     6121 gtggtcagca tgtgttgctg attatggact ccctcacccg ctacgcgatg gcccagcgtg
     6181 aaattgcgct ggcgattggc gaaccacctg ctactaaagg ttatccaccg tcggtgtttg
     6241 ccaaattacc ggcactggtc gagcgtgccg gaaatggcat tagcggcggc ggctcgatta
     6301 ccgcgtttta taccgtgctc accgagggcg atgaccagca ggaccccatt gccgactccg
     6361 cgcgggccat cctcgacggt cacattgtgc tgtctcgccg actggcggaa gccgggcact
     6421 atccggctat cgatattgaa gcgtcgatca gccgcgcaat gacggcgttg atcagtgagc
     6481 aacattacgc acgagtgcgc accttcaaac agctgttgtc gagttttcag cgtaaccgag
     6541 atctggttag cgtcggcgcg tatgccaaag gcagcgatcc gatgctcgac aaagccatcg
     6601 ccctgtggcc gcagctggag ggctatttgc aacaaggcat ttttgaacgc gcggactggg
     6661 aagcgtctct ccaggggctg gagcgtattt tcccgacagt gtcataaccc aggagataac
     6721 ggcagatggc agaacatggc gcgctggcga ccctgaaaga tctggcagaa aaagaggtag
     6781 aggatgccgc gcgcctgctg ggtgaaatgc gtcgcggatg tcagcaggcg gaagaacagc
     6841 tcaaaatgct gatcgattat cagaatgaat atcgcaataa cctcaatagc gatatgagtg
     6901 ccgggatgac cagcaaccgc tggatcaact atcagcagtt tatccagacg ctggaaaaag
     6961 ccattactca gcatcgccag caacttaatc agtggacgca gaaagttgac attgccctga
     7021 acagctggcg agaaaagaaa caacgtttgc aggcctggca gacactgcag gaacggcaat
     7081 ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa aaagatggat gagttcgccc
     7141 agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg cccttaatta ccgccgacgt
     7201 tgacaccacc acattgcctg gcggcaaagc cagcgatgct gcacaagatt ttctcgcgtt
     7261 gttgagcgaa gcattagcag gcgagacaac taccgacaaa gcggcccccc agttgctggt
     7321 ggcaacagat aagcccacga caaaaggcga gccgctgatc agcgatattg tttccgacgc
     7381 gcaacaagcg gatttactga tccccgtgga tgaaacactg cctgtcatca acgacgaaca
     7441 atccacatca acaccgttaa ccaccgctca gacgatgacg ttggctgcgg tggctgacaa
     7501 aaatacgaca aaagacgaaa aagcggatga tcttaatgaa gacgtcaccg caagcctgag
     7561 cgcccttttt gcgatgttgc cgggttttga caatacgccc aaagtgactg atgcaccgtc
     7621 aaccgtgtta ccggcagaga aaccaacgct tttcacaaaa ctgacttctg cgcaactcac
     7681 aacagcacag cctgatgacg cccccggcac accagctcag ccattaacac cgctggtagc
     7741 agaagcccag agtaaagcgg aagtcatcag cacaccttcg ccggtgactg ctgccgccag
     7801 cccgctaatc actccacacc agacacagcc actgcccacc gtcgccgcgc ctgttttgag
     7861 tgcaccgctg ggttctcacg aatggcaaca atcattaagc cagcatattt cgctgttcac
     7921 ccgccagggg caacaaagtg cagagttgcg tctgcaccca caggatttag gtgaagtgca
     7981 aatttccctc aaagtggatg ataaccaggc gcagatccag atgatttcac cgcatcagca
     8041 cgtacgcgca gccctggaag cagcgctgcc ggtactgcgt acgcagctgg ccgaaagtgg
     8101 cattcagtta gggcaaagca acattagtgg cgaaagcttt agtggtcagc agcaggccgc
     8161 ttcccagcaa cagcaaagcc aacgcacagc aaaccatgaa cctctgacgg gggaagacga
     8221 cgatacgctt ccggttcccg tctctttaca agggcgtgta acaggcaaca gcggcgttga
     8281 tattttcgcc taacgtcaga ggtagcaccg taatccgcgt cttttccccg ctttgttgcg
     8341 ctcaagacgc aggataatta gccgataagc agtagcgaca caggaagacc gcaacacatg
     8401 actgattacg cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat tctggtattc
     8461 attaccctcg cggcctgtgc cagcgcaggt tacagctact ggcattcgca tcaggttgcc
     8521 gctgacgaca aagcgcagca acgcgtcgtg ccctccccgg tcttctatgc gctggatacc
     8581 tttacggtca atttgggcga tgcggatcgc gtgctttata tcggcataac cctgcgactg
     8641 aaagatgaag caacccgctc gcggctgagt gagtatttgc cggaagtccg tagtcgcttg
     8701 ctgttactgt tttcacgtca ggatgctgcc gtactggcga cagaagaagg caagaaaaac
     8761 ctgattgccg agattaaaac cacactttcc accccgcttg ttgccgggca accgaaacag
     8821 gatgtcaccg acgtgctgta taccgctttt attctgcgat aacgacatgg gcgatagtat
     8881 tctttctcaa gctgaaattg atgcgctgtt gaatggtgac agcgaagtca aagacgaacc
     8941 gacagccagt gttagcggcg aaagtgacat tcgtccgtac gatccgaata cccaacgacg
     9001 ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat gagcgctttg cccgccattt
     9061 tcgtatgggg ctgttcaacc tgctgcgccg tagcccggac attaccgtcg gggccatccg
     9121 cattcagccg taccatgaat ttgcccgcaa cttgccggtg ccgaccaacc tgaaccttat
     9181 ccaccttaaa ccgctgcgcg gcacagggct ggtggtgttc tcgccgagcc tggtgtttat
     9241 cgccgtggat aacctgtttg gcggcgatgg acgcttcccg accaaagtgg aaggtcgcga
     9301 gtttactcat accgaacagc gcgtcatcaa ccgcatgtta aagctggcgc ttgaaggcta
     9361 tagcgacgcc tggaaagcga ttaatccgct ggaagttgag tacgtgcgtt cggaaatgca
     9421 ggtgaaattt accaatatca ccacctcgcc gaacgacatt gtggttaaca cgccgttcca
     9481 tgtggagatt ggcaacctga ccggcgaatt taatatctgc ctgccattca gcatgatcga
     9541 gccgctacgg gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg aagatcagaa
     9601 ctggcgcgat aacctggtgc gccaggtgca gcattcacag ctggaactgg tggccaactt
     9661 tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg aaacccggcg acgtcctgcc
     9721 gatagaaaaa cccgatcgca tcatcgccca tgttgacggc gtcccggttc tgaccagtca
     9781 gtacggcact ctcaacggtc agtatgcgtt acggatagaa catttgatta acccgatttt
     9841 aaattctctg aacgaggaac agcccaaatg agtgacatga ataatccggc cgatgacaac
     9901 aacggcgcaa tggacgatat gtgggctgaa gcgttgagcg agcaaaaatc aaccagcgga
     9961 aaaagcgctg ccgacgcaat gttccagcaa tttggcggcg gagatgtcag cggaacgttg
    10021 caggatatcg acctgattat ggatattccg gtcaagctga ccgtcgagct gggccgtacg
    10081 cggatgacca tcaaagagct gttgcgtctg acgcaaggat cagtcgtggc gctggacggt
    10141 ctggcgggcg aaccgctgga tattctgatc aacggttatt taatcgccca gggcgaagtg
    10201 gtggttgttg ccgataaata tggcgtgcgg atcaccgata tcattacccc gtccgagcgg
    10261 atgcgccgcc tgagccgtta gtgatgaata accatgctac tgtgcaatct tccgcgccgg
    10321 tttctgctgc gccactgctg caggtgagcg gcgcactcat cgccattatt gccctgatcc
    10381 tcgctgccgc ctggctggta aaacggctgg gatttgcccc taaacgcact ggcgttaacg
    10441 gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga acgggtcgtg gtggtcgatg
    10501 tggaagatgc acggctggtg ctcggcgtta ccgcagggca aatcaatctg ctacataaac
    10561 ttcccccttc agcaccaacg gaggagatac cgcagaccga ttttcagtcg gtcatgaaaa
    10621 atttgcttaa gcgtggcggg agatcctgat gcgtcgtttg ttttctgtcg caccggtcct
    10681 tctctggctg attacgcccc tcgccttcgc gcaactgccg ggtatcacca gccagccgct
    10741 acctggcggt ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca tcacctcgtt
    10801 gacgttcatt ccagcgattt tactgatgat gaccagtttc acccgcatca ttattgtttt
    10861 tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca cctaaccagg tattgctggg
    10921 actggcactg tttttgacct tttttattat gtcgccagtg atcgacaaaa tttatgtcga
    10981 tgcgtaccag ccattcagcg aagagaaaat ctcaatgcag gaggcgctgg aaaaaggggc
    11041 gcagccgctg cgtgagttta tgctgcgtca gacccgcgag gcggatttag ggttgtttgc
    11101 cagactggcg aataccggcc cgttgcaggg gcctgaagct gtgccgatgc gcattttgct
    11161 cccggcctac gtgaccagcg agttgaaaac cgcatttcag ataggcttca cgattttcat
    11221 cccttttttg attatcgacc tggtgattgc cagcgtgttg atggcattgg ggatgatgat
    11281 ggttccccca gccaccattg ctctgccctt taaactgatg ctgtttgtac tggtggatgg
    11341 ctggcaattg ctggtcggtt cgctggcgca gagcttttac agctagagag gcaaaatgac
    11401 acctgaatcg gtcatgatga tggggactga agcgatgaaa gtcgcgctgg cactggctgc
    11461 cccactgttg ctggtggcgc tggtcacggg tcttatcatc agtattttgc aggccgccac
    11521 gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc atcgccgtat ttatcgccat
    11581 tattattgcc ggaccgtgga tgctcaatct gttgctggat tacgtccgca ccttgttcac
    11641 taacctgccg tatatcatcg ggtaggccgt acaatgatgc aggtaacaag cgatcaatgg
    11701 ctttcctggt taagcctgta cttctggccg ttactgcgcg tgctggcgct catttctacc
    11761 gcgccaattc tgagcgaacg cagcgtgccg aaacgggtaa aactgggtct ggcaatgatg
    11821 atcacgttcg ccattgcccc atcattacct gccaacgatg ttcctgtttt ttcgttcttt
    11881 gctctgtggc tggccgtgca gcagatcctg atcggcattg cgcttggttt taccatgcaa
    11941 tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat ggggctgtca
    12001 tttgcgacat ttgtcgatcc tggcagccat ctcaatatgc ccgttttagc gcgcatcatg
    12061 gatatgctgg cattactgct gttcctgaca tttaacggtc atttatggtt gatttcactg
    12121 ctggtcgata cctttcacac cctgccgatt ggtagcgaac cgttgaacag caatgcgttt
    12181 ctggcactca ccaaagcagg gagtttgatt ttccttaacg ggctgatgct ggcgttaccg
    12241 ctcattactc tgttgctgac cctgaatctg gcattaggtt tactgaatcg tatggccccg
    12301 caattatcca tttttgttat tggatttcca ttaactctga ctgtcggcat ctctttaatg
    12361 gcggcattaa tgccgttaat tgcgcctttt tgcgaacatt tattcagtga aatttttaat
    12421 ttgctggctg atattattag tgaattacca ttaatataat tccgtaacgt tta
//
LOCUS       DACADF010000135        12374 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.137, whole genome shotgun sequence.
ACCESSION   DACADF010000135 DACADF010000000
VERSION     DACADF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.137"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..92)
                     /locus_tag="ISB30_003734"
     CDS             complement(<1..92)
                     /locus_tag="ISB30_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP1991326.1"
                     /translation="MLRPSRSRRFHRAGEGLPALCGATPFRARA"
     gene            complement(325..645)
                     /locus_tag="ISB30_003735"
     CDS             complement(325..645)
                     /locus_tag="ISB30_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991327.1"
                     /translation="MRMSLMFAAVTLLTGINLFFIPFQPVPALWGAILAVAFVLINVV
                     ADKLRLWSGIAWIIVAGIIITLMLKGKEPGDELRGLITVFAFLASAWYCVVQYRKEQG
                     ESQS"
     gene            complement(680..934)
                     /locus_tag="ISB30_003736"
     CDS             complement(680..934)
                     /locus_tag="ISB30_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAP1991328.1"
                     /translation="MSKWNIASFSKEEQDKVAVDKVAAAVAWQERMNKPVVPELVERE
                     QPEHLREYFHERLRVHRLNSQQLPRANAPEYNKPGDEQQK"
     gene            complement(1122..1313)
                     /locus_tag="ISB30_003737"
     CDS             complement(1122..1313)
                     /locus_tag="ISB30_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991329.1"
                     /translation="MKLAPQSRDILRQYKALINARRRDAGQRELTTAQVMDEICEYMT
                     CQCAVYIGGHFILQGGKAR"
     gene            complement(1356..1862)
                     /locus_tag="ISB30_003738"
     CDS             complement(1356..1862)
                     /locus_tag="ISB30_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP1991330.1"
                     /translation="MTSSVTVPAVYVGTYHQYNGGSIFGKWFDLTDFDDEDEFYDACR
                     ALHAAEDDPEFMFQDWEGIPSQFASESSVKWAFIEAFRQAQDEGRAAAFVAWADYTGE
                     CDYDAFDEAYCGEAESEEDFAYGFVEDHGLLNEVPESLRVYFDYEAYARDLFSSGYVF
                     HEGYVFSN"
     gene            complement(1905..2570)
                     /locus_tag="ISB30_003739"
     CDS             complement(1905..2570)
                     /locus_tag="ISB30_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
                     /protein_id="HAP1991331.1"
                     /translation="MQGCADAAILARSQVFYDARRSSRRFLAQVSLENRPDQLRAISA
                     LRPPVSPLHLPLFAGLSCRHGTTRCRSLCDYGGLSMQYVNTCPVLNVEESDRLSFMPY
                     LFGTDFMLAELQVYALAKKWMPEYEGGFWHFIRLPEGGGYMMPDCGRVHLVSSENWFD
                     RTVSADAAGIILTSLAINRRLAAHHDSSNPALTRLYMLRDAQLWNHITFHPECSAIYA
                     ALD"
     gene            complement(2585..2941)
                     /locus_tag="ISB30_003740"
     CDS             complement(2585..2941)
                     /locus_tag="ISB30_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991332.1"
                     /translation="MSPCRLRVHPARFPGLLGEHNPLLSPCRVLAVPCLHFSPAAAEA
                     GVRWRRHPRIRQSPACRCRRLRFTRPHPSGPWPYYCVCRVATSASAFLPVVEKRGRET
                     LYSTSCIATGSAVPSL"
     gene            complement(3014..3781)
                     /locus_tag="ISB30_003741"
     CDS             complement(3014..3781)
                     /locus_tag="ISB30_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991333.1"
                     /translation="MMKKETLNTLILRHGDRMLRQAGWPDTVDMAPVAPETVPGWLAA
                     CGSLSGVEILTLTEQLCQPLTYGRAALLLASARRLAGTPARLHLFPVQAYPHPERLAD
                     CQVIRLPYAQEWLTAAECDDLLAFLKASLTQISEIVHRDTKRIAAALTPSVNPRLMDR
                     RIGDWRLLAVEYDHDNCLDEDETDRLDQVLDAILIRDARFCPVLLTLVNEREETIRSA
                     GVIADQLRFTDTPVRRWFDRRVLREMVREARASRTQE"
     gene            complement(3835..4254)
                     /locus_tag="ISB30_003742"
     CDS             complement(3835..4254)
                     /locus_tag="ISB30_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAP1991334.1"
                     /translation="MYGTREDLCRMLNEQYPAETPLNLIIWTPADIEALADGMEYSFS
                     EHDVRAVLERMDTIPEEQRLESGVSAGLVMALIDQVKENGQRVTVPVDLLETLLITAE
                     QALWDREWTARDRNLPVPESVMRRLADTAKVRALLKS"
     gene            complement(4264..4485)
                     /locus_tag="ISB30_003743"
     CDS             complement(4264..4485)
                     /locus_tag="ISB30_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991335.1"
                     /translation="MNYDGHEALRRDMAGLANNLCDLKTTLKVLEDTYHYRYDGLAER
                     LAGISLRRLSVLMDEAFNIALMLDESFLD"
     gene            complement(4485..5186)
                     /locus_tag="ISB30_003744"
     CDS             complement(4485..5186)
                     /locus_tag="ISB30_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAP1991336.1"
                     /translation="MSRFILGNCIDVMRGFPDRAVDLIVTDPPYLVGFKDRQGRQIAG
                     DVTDEWLQPATLEMYRVLKKDALMVSFYGWNRVDRFMAAWKAAGFYAVGQLVFTKNYA
                     SNRRNARARRGFVDYCHEGAYVLAKGRPVPPLKPLPDVLPFPYTGNTLHPTQKPVEAL
                     QPLIESFSAPGAIVLDPFAGSGSTCVAAYRAGRRYIGIEMLAQYHRAGTERLAAMHRA
                     VNTPAANDEWLPEAA"
     gene            complement(5388..5504)
                     /locus_tag="ISB30_003745"
     CDS             complement(5388..5504)
                     /locus_tag="ISB30_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991337.1"
                     /translation="MPVIPRSPGVNASLPPAASVSRPVWYGTGGFSAPARRR"
     gene            complement(5623..5853)
                     /locus_tag="ISB30_003746"
     CDS             complement(5623..5853)
                     /locus_tag="ISB30_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991338.1"
                     /translation="MSPGFRNVLRLARIPVDSSEPVRRKFGSRLIGIVTVCLLPLLVF
                     DVPQWVFILSQVIWGAGVYIGLSLAGDKSLNR"
     gene            complement(5917..6588)
                     /locus_tag="ISB30_003747"
     CDS             complement(5917..6588)
                     /locus_tag="ISB30_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mediator of plasmid stability"
                     /protein_id="HAP1991339.1"
                     /translation="MPDGQYSFYLHSDDRTDIAAMATISTISQPLRGEFIRTAATAGA
                     VVYQVDARLPALIPVFFAGQLSAVRLCAVMALVSGTWSSLTGLPDEPENGEAALPVSP
                     ETEYQRRRYTLTLQNGRSGERVESVLTEASSRLRGDLLRNLIITGLALHTTAPELPRL
                     LASMPVPPTSVSELQALVQQMAGTADVNRAAAAVPDTPAASAAPVSGADTREIKKNMR
                     RAFGD"
     gene            complement(6591..7562)
                     /locus_tag="ISB30_003748"
     CDS             complement(6591..7562)
                     /locus_tag="ISB30_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbA family protein"
                     /protein_id="HAP1991340.1"
                     /translation="MNIYCDDGSTNVKLAWFEGDELQTRVSANSFRHGWKVAEFSAAT
                     FNYQVGTLKYTWDSVSRDAIPTTNVEYQYGDLNLLAVHHALLNSGLEPQPVSLTVTLP
                     LSEYYDGDCQRNEENIRRKRENLMRELVLNKGRAFTVTDVKVMPESLPAAFSRLAELK
                     PGPAETTLIIDLGGTTLDAGVIVGQFDDISAVHGNPSVGVSQVTRAAAGALRAADSET
                     SALIADTIIRNRNDRQYLQRVINDAGKIDEVRNKITEAITSLGARVTSELTAFRNVNR
                     VFLVGGGASLIEEAIRQAWPLAPDRIEVIGDPQMALAREIALYNKED"
     gene            7796..8227
                     /gene="umuD"
                     /locus_tag="ISB30_003749"
     CDS             7796..8227
                     /gene="umuD"
                     /locus_tag="ISB30_003749"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAP1991341.1"
                     /translation="MKLQLFSTGKELPPQAHPLFADLASCGFPSPAADYVESDLDLHD
                     YCIRHPSATYYLRASGDSMADGSLYNGDLLVVDSAEKPRHGDIVVASVQGEFTVKRLL
                     LTPRLTLQPMNASWSPIYPDPDELDIFGVVTHIIHRPREMY"
     gene            8227..9498
                     /locus_tag="ISB30_003750"
     CDS             8227..9498
                     /locus_tag="ISB30_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP1991342.1"
                     /translation="MFALADVNSFYASCERVFRPDLKGKPIVVLSNNDGNVIARSAEA
                     KPWIKMGTPWFQIKNERYPEKIYAFSSNYELYASMSARVMNCLEELAPRVEQYSIDEM
                     FLDLTGVEHCMGLEDFGRQLRQHVYDCTRLTIGVGAGPTKTLAKSAQWASKEWKQFRG
                     VLALTRGNPQRTRKLLSLQPVEEIWGVGNRIARKLNVLGIKTALDLALTNPAFIRKNF
                     SVVLERTVRELNGESCLSLEEAPPTKQQIVCSRSFGVKITEYESLRQAICQHAERASE
                     KLRKEHQYCRHISVFIKTSPFAIKEPYYGNVATEKLLTPTQDTRDIIAAATMALERIW
                     RDGHRYAKAGVMLNDFTGSGVSQLQLFDERPPRPHSAELMRVLDGINNSGLGKVWFAG
                     RGIAPSWQMKRDMLSPAYTTRWRDIPVARIG"
     gene            9910..10785
                     /locus_tag="ISB30_003751"
     CDS             9910..10785
                     /locus_tag="ISB30_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP1991343.1"
                     /translation="MNENVYIGEVIGPDCESVNNKLTVTSKATVQPVALMRLNVFVPS
                     SRPAKGTRTSIDATSVLKDLEFSRREGYDDIRITGERLSMAIDFKVWVGIIEAFSKYG
                     LKTNSIKLTFHEFAGMCRFESKRFDSRLRKSISEALTRIRGKTITFTRSDSEGLQKVS
                     VTGLLKTGELDFANDQVELEADQRLWELYQTDYTVLLRHKPINALTRKEAAQALYTFI
                     ESLPTNPVPISFKRIRDRLSLLSPVKEQNRIIKLALKQLQDIGYLDHSITKKGKETFI
                     LIHRRSANLKPGTAV"
     gene            11450..12076
                     /locus_tag="ISB30_003752"
     CDS             11450..12076
                     /locus_tag="ISB30_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HAP1991344.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANQAADVLKD
                     YPEMRLASSRVCDRKAHRDAWAESMTIFETQNDKAQQEIESLVKEVIL"
     gene            12073..>12374
                     /locus_tag="ISB30_003753"
     CDS             12073..>12374
                     /locus_tag="ISB30_003753"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991345.1"
                     /translation="MRNRANTPSRDEAAFINGGTAGIDNTKVTSERPLAVSKAKPVSI
                     SFFEENLKDIDDLITGEMISGKSRVNRSDVVRAAVEALKSLSKTEISRLIAEAKQR"
BASE COUNT         2987 a         3339 c         3343 g         2705 t
ORIGIN      
        1 aacgcgcggg cgcgaaacgg cgttgccccg cagagtgccg gcagtccttc gccggcacgg
       61 tgaaagcggc gcgaccggga cggtcgcagc aagcgctgcc gttgaccttg acgtcagaca
      121 tcagacacta gccgccaggc ccgaaaccgc gtagcgggtt cggcggagct taccgcgtct
      181 tttcgcggtt agcggagtgt ggctcgacga ctgaaccgcg ccagcgggtc aggagccgaa
      241 ggtggccccg gagcagattg tgtgaaagga aagtgaggca aagccagagg aagagcagca
      301 gcaagatgaa gggtaccggt caggtcagga ctgagattcg ccctgctctt ttctgtactg
      361 tacaacgcag taccaggccg aagccaggaa agcaaaaacg gtgatcaacc cgcgtaattc
      421 gtcacccggc tctttcccct tcagcatcag cgttatgatg atgccggcaa cgataatcca
      481 cgcaatgcca ctccagaggc gcagcttatc cgccacgaca ttaatcagaa caaaagcaac
      541 ggccaggata gccccccaca gagccggaac aggctgaaac ggaataaaaa acaggttaat
      601 accggttaaa agcgtcacgg ctgcaaacat cagagacata cgcataaaat ttcctccctg
      661 gacacactga ctataagaat cacttctgct gctcatctcc gggtttatta tattcgggcg
      721 catttgcgcg cggcagctgc tgactgttca gtcgatgtac gcggagccgt tcatggaaat
      781 attcgcgcag gtgttcaggc tgttcccgtt caacaagttc cggcacgacg ggcttgttca
      841 tgcgctcctg ccaggcaaca gcagcagcca ccttatcgac ggcaacttta tcctgctcct
      901 cctttgaaaa agaggcgata ttccactttg acatatgctt taccggtctg ctttacagga
      961 agatatccct gataatacgc gaattccggg gcaacagcga ctgacaacgg agaggagagt
     1021 gcgcggcgcc ggaggcgcgg cgacgtctct ctccggcagg cgcagcctgc ccgggttttc
     1081 gcgcggccgg aagcggcaga ggttcccccc tgccctgtga tttatcgtgc ctttcccccc
     1141 tgaagaatga aatggccgcc aatatagacg gcgcactgac aggtcatgta ttcgcagatt
     1201 tcatccatca cctgcgcagt ggtcagttcc cgctgaccgg catcgcggcg gcgggcatta
     1261 atcagggctt tgtactgtcg caggatatcg cggctttgtg gtgcaagttt catgtgcttt
     1321 ctccttaacc ggcgggcttc cccgccggat agtcatcagt tgctgaatac atagccttca
     1381 tggaagacat agccgctgct gaacagatcc cgcgcgtaag cctcataatc gaaatagacg
     1441 cgcagggatt caggcacctc gtttaacagg ccgtgatcct ccacaaagcc ataagcaaaa
     1501 tcctcctcgc tttcagcctc gccacaatac gcctcatcaa aggcgtcata gtcgcactcg
     1561 ccggtatagt ccgcccaggc cacaaacgcg gcagcgcgtc cttcgtcctg cgcctgccgg
     1621 aatgcctcga taaacgccca cttaacagag gattcagagg caaactgaga cgggatccct
     1681 tcccagtcct gaaacataaa ctcggggtca tcctcggcgg catgcaacgc ccggcaggcg
     1741 tcataaaatt cgtcttcgtc gtcgaagtcg gttaaatcaa accatttgcc aaaaatactg
     1801 ccgccgttgt actggtgata tgtgcccacg tagacggccg gaacggttac tgatgaagtc
     1861 atttttgtct ccctgcgggg cggtgtaacc gccccggtcg ttaatcagtc cagcgcggcg
     1921 taaatagcac tgcattcggg gtgaaaggtg atgtgattcc agagctgggc atcacgcagc
     1981 atataaaggc gagtcagcgc cgggttactg ctgtcatgat gggccgccag acggcgatta
     2041 atcgccaggc tggtcagaat aatgcccgcc gcgtcggcgc tgacagtgcg atcaaaccag
     2101 ttttcgctac tgaccagatg tacccggcca caatccggca tcatgtagcc gcccccctcc
     2161 ggcaggcgaa taaaatgcca gaagccgccc tcatattcgg gcatccattt tttggccaga
     2221 gcgtacacct gtaattccgc gagcatgaaa tccgtgccga acaggtacgg cataaaagac
     2281 aggcggtcgg actcctcaac attcaggacc gggcatgtat tcacatactg catggataaa
     2341 cctccgtaat cacatagact gcggcaccgg gttgtgccgt gccgacacga cagcccggcg
     2401 aaaagcggaa gatgcagggg cgacaccgga ggccgcagcg cagatatagc gcgaagctgg
     2461 tctggacggt tttcgagcga aacctgagcg agaaaacgac gggaagaacg gcgagcatca
     2521 taaaaaacct gcgaccgggc gaggatggcg gcgtcagcgc acccttgcat ctgcagcggc
     2581 cgggctaaag tgaaggcacg gcactacccg tcgcgatgca ggaggttgaa tacagggttt
     2641 ctctcccccg tttttcgacc accgggagaa aagcggaagc agatgtcgca acgcgacaga
     2701 cgcagtaata aggccacgga ccggagggat ggggccgggt gaagcggagc cggcggcaac
     2761 ggcaagccgg cgactgcctt atccggggat ggcggcgcca gcgaactcca gcctctgcgg
     2821 ctgccgggct aaagtgaagg cacggcacag ccagcacacg gcacggcgat aacagagggt
     2881 tgtgttcacc caaaaggccc ggaaaccggg ccgggtggac gcggagccgg cacggcgaca
     2941 tccttatcgc accacacctc ctgtgccggc aggctgacaa gggagggtcc ccctcccctg
     3001 acgctccggg ctgtcactcc tgagttctgc tggcgcgggc ctctctcacc atttccctta
     3061 aaacccggcg atcaaaccag cgccggaccg gagtgtccgt aaaacgcagc tgatcagcga
     3121 taacccctgc gctcctgata gtttcctccc gctcgttaac cagggtgagc aggaccgggc
     3181 agaatcgcgc atcgcgaatc agaatggcat cgaggacctg atccagcctg tcggtttcgt
     3241 cttcgtccag gcagttatca tgatcatact ccacggccag caagcgccag tcaccgatcc
     3301 gcctgtccat cagacgggga ttgacggacg gcgtaagcgc ggcggcgatg cgcttcgtat
     3361 cccggtggac gatttccgat atctgcgtga gggaggcttt caggaaggcc agcagatcgt
     3421 cacattccgc cgctgtcagc cactcctgag cgtaaggcag gcggataacc tgacagtcag
     3481 ccagccgctc cggatgcgga tatgcctgca ccggaaagag atgcagacga gccggggttc
     3541 ccgccagccg ccgggcgctg gcgagcaaca acgctgcccg gccatacgtc agcggctgac
     3601 aaagctgttc cgtaagcgtg aggatttcca cgccactgag agagccgcag gcggcaagcc
     3661 agccgggtac ggtttcgggg gcgacgggcg ccatatcaac agtgtcgggc cagccagcct
     3721 ggcgcagcat ccggtcgccg tgacgcagaa taagcgtgtt aagggtttct tttttcatca
     3781 ttatcgtctc tgtccgggtt atatcacccc gccgcgtggc ggggcgatct gctgtcagct
     3841 tttcagtaat gcccgcactt ttgctgtatc cgccaggcgg cgcattacgc tttccggcac
     3901 cgggagattg cgatcccgtg cggtccattc cctgtcccac aatgcctgtt cggcggtaat
     3961 cagcagggtt tcaagcaggt caaccggcac agtcaccctc tgcccgttct ctttcacctg
     4021 gtctatcagt gccatcacca ggccggctga cacgcccgac tcaagccgtt gttcttccgg
     4081 tatagtgtcc atgcgttcca gtactgccct tacgtcatgt tctgaaaagg aatattccat
     4141 cccgtcagcc agtgcttcaa tatccgccgg ggtccagata atcaggttca gcggggtttc
     4201 tgcgggatat tgctcattca gcatcctgca aagatcttca cgggttccgt acatgttctc
     4261 tcctcaatcc agaaagcttt catcgagcat cagtgcaata ttgaacgcct catccatcag
     4321 gacgctgaga cgtcgcagac tgataccggc aagccgttcc gccagtccgt cgtaccggta
     4381 gtggtacgtg tcttccagca ctttcagggt ggttttcagg tcgcaaagat tgttcgccag
     4441 cccggccata tcgcggcgca gggcttcgtg tccgtcgtaa ttcattacgc ggcctccgga
     4501 agccattcat cgttcgccgc cggggtattt acggcccggt gcatggcggc cagtcgttcg
     4561 gttccggccc ggtggtactg cgccagcatt tcgatgccga tatagcggcg tccggcacgg
     4621 tatgcagcga cgcaggtcga accgcttccg gcaaacgggt cgagaacaat cgcgcccgga
     4681 gcgctgaatg actcaatcag cggctgtagc gcctccactg gtttctgcgt cggatgcagg
     4741 gtattaccgg tatacgggaa gggcagcaca tccggcagcg gtttaagggg cggcacgggg
     4801 cggcctttgg caaggacata agccccttca tggcagtagt ccacaaatcc gcgtcgggcc
     4861 ctggcgttac gacggttgga ggcatagttt ttggtgaaca caagctggcc cacggcatag
     4921 aatcctgccg ccttccacgc ggccataaag cggtccacac ggttccagcc gtagaagctc
     4981 accatcagcg cgtctttttt aaggacgcgg tacatctcca gcgtggccgg ttgcagccat
     5041 tcgtccgtca catccccggc aatctgtctg ccctgacggt ccttaaaacc gacgaggtaa
     5101 ggcggatcgg tcacaataag gtccacggca cggtcgggga atccgcgcat cacatcaata
     5161 cagttcccga gaatgaatct ggacatggca gcactcctgt agtctgaggg ggcaggaggc
     5221 atcaccgcga taccgttccc tgccgcacgc tgcctgttca tcgcgtccgg tcacgggtca
     5281 ggccgaccgt ggaataccgc agccgtccgt cttttggacg gcaaggatat gccgcgaaag
     5341 cggcttgaag ggtgttcctt aaaagggcga gggactggcg tacactctca acgacgccgg
     5401 gccggagcgg aaaaaccgcc cgtcccgtac cagaccggac gcgagacact ggcagcgggc
     5461 ggcaggcttg cgttaactcc aggcgaacgg ggaattaccg gcatacctgt ccacaaatcc
     5521 ggggcttaag attgtggata acctgaaggc aaacgacgga gaagcgcggg agaacagacg
     5581 ccaccacgcc tgttcttttt ttgaacaacc ggaaagttta tatcagcggt tcagggattt
     5641 atccccggcc agtgacaggc caatatagac gcccgccccc cagatgacct gcgacaaaat
     5701 gaaaacccac tgcggaacgt cgaaaaccag aagcggaagc agacataccg tcacaatgcc
     5761 aatcagccgg ctgccaaatt ttctgcgcac cggttcactg ctgtcgacgg gaatgcgggc
     5821 gagacggaga acatttctga atcctggtga catgagaacc tccctgggga tgtcagtcat
     5881 ttgaaatcgg tatagtagtg gatgtgattc gtactgttag tcaccgaatg cacgccgcat
     5941 atttttcttt atttcccggg tatccgcccc ggacaccggc gcggcagatg ctgccggcgt
     6001 atccggtact gcagctgcag ccctgttcac gtcagcggtg ccggccatct gctgaaccag
     6061 agcctgcagc tcactgaccg aggtgggcgg taccggcata ctggccagca ggcgcggcag
     6121 ttcaggcgcc gtcgtgtgga gggcaagacc ggtgataatc aggtttctca gcagatcccc
     6181 acgcagacgg gatgacgctt ccgtcagcac actttccacc cgttccccac tccggccatt
     6241 ctgaagcgtc agggtgtaac gccggcgctg gtattctgtc tccggtgaca cgggcagagc
     6301 tgcctcacca ttttcgggct catccggaag gccggtgagt gaggaccagg ttccggagac
     6361 cagggccatc acggcgcaaa ggcggacagc gctcagttgc ccggcaaaga acaccgggat
     6421 cagcgccggt aaacgggcat ccacctggta taccaccgcc ccggcggtgg cggcggtacg
     6481 gatgaactca ccgcgcaggg gctgtgagat ggtgctgatg gttgccattg ccgcgatgtc
     6541 ggtgcgatcg tcgctgtgca gatagaaaga atactggccg tccggcacgt ttagtcctct
     6601 ttattgtaaa gggcaatctc cctggccagc gccatctgcg gatcgcctat aacctcgata
     6661 cggtcaggag ccagaggcca ggcctgacgg atagcttctt ctatgaggga agcccctccc
     6721 ccgaccagga atacccggtt aacgttcctg aaggccgtca gctcactggt gacacgcgcc
     6781 cccagggaag tgatcgcctc tgttattttg ttccgaacct cgtcgatttt accggcatcg
     6841 ttgatcacac gctgcagata ctgacgatca ttgcggttgc ggatgatagt gtcagcgatg
     6901 agggcgctgg tttcgctgtc tgccgcacgc agcgcgcctg cagctgcgcg ggtgacctgt
     6961 gacacgccga cagacggatt accgtgcacc gcactgatat catcaaactg gccgacaatc
     7021 acaccggcat cgagcgtggt accaccgaga tcgataatca gggtggtctc agcggggccg
     7081 ggtttcagct ccgcgagacg ggagaacgct gccggtagtg attccggcat caccttcacg
     7141 tccgttacgg taaatgccct gcccttattc agaaccagct cacgcatcag gttttcccgt
     7201 ttgcgtcgta tgttctcctc gttccgctgg cagtccccgt cgtaatactc actcaggggc
     7261 agcgtcacgg tcaggctcac cggctgcggc tccagtccgc tgttcagcag cgcatgatgc
     7321 acggccagca ggttgagatc accgtactga tattccacgt ttgttgtcgg gatggcatcc
     7381 cgactgacgc tgtcccaggt gtatttcagc gtccccacct gatagttgaa cgttgccgca
     7441 ctgaattccg ccactttcca gccatgccgg aaggagttcg cagaaacccg ggtctgaagc
     7501 tcatcgcctt caaaccatgc cagcttaaca ttagttgaac catcatcgca gtaaatattc
     7561 atcaatatgc tccgttagtt aaactcaatt tgataccaat tataagagca aaatgaaaaa
     7621 aacaaagtaa catttctcaa tatgagcaat ataaaaatat caaaatgatt aatataagca
     7681 ttttgaacac ctttatttac tcaaaatgtt cttaaggcat gcgatctgga ttatcgtcac
     7741 gctgttaaat atactgtatg cataaccagt aaaaattaga gtgtcaggag gaaagatgaa
     7801 actacagctt ttcagcaccg ggaaagaact cccgccacag gcccatccat tgtttgccga
     7861 tctggccagc tgcggttttc ccagccctgc tgctgactat gtcgagtccg atctggatct
     7921 ccatgattac tgcatacggc atccgagcgc gacgtactac ctgcgtgcca gcggagacag
     7981 catggccgac ggcagtctgt acaatggcga tctgctggtt gtggacagtg ctgaaaagcc
     8041 gcggcacggc gatatcgttg tcgccagcgt acagggggag tttacggtga aacggcttct
     8101 gctgacgccg cgactgacgc tgcagcccat gaatgcctcc tggtcgccga tttatcctga
     8161 tcccgacgag ctggatatct ttggcgttgt cacgcatatc atccaccgcc cgagggagat
     8221 gtactgatgt ttgccctggc cgatgtgaac agcttctatg ccagctgtga acgtgttttc
     8281 cgcccggatt tgaaaggaaa gccgatagtc gtcctgagca acaacgacgg gaacgtaatc
     8341 gctcgcagtg ccgaggcgaa gccctggatc aaaatgggaa ctccgtggtt tcagataaaa
     8401 aacgaaaggt acccggaaaa aatatatgcg ttttcaagta attatgaact ttacgcctcg
     8461 atgtcggcgc gcgtgatgaa ctgcctggag gaactggccc ccagggtgga acagtattcc
     8521 attgatgaga tgttcctcga cctgaccggc gtggagcact gtatggggct tgaggacttc
     8581 ggccggcagc tgcgtcagca cgtgtatgac tgtacccgcc tgaccattgg cgtgggcgcc
     8641 ggaccaacga aaaccctcgc aaaatcagcc cagtgggcct caaaagagtg gaaacagttt
     8701 cgtggcgtac tcgcgctgac caggggaaat ccgcaaagga cgcggaaact gctttcactg
     8761 cagccggttg aagaaatctg gggggtgggt aaccgtattg cacgcaagct aaatgtgctg
     8821 ggcataaaaa ccgcgctcga tctggctctg acgaacccgg ccttcatccg caaaaatttt
     8881 tctgtggtcc ttgagcgaac ggtacgggaa ctgaacgggg aaagctgcct ttcgcttgag
     8941 gaagcccctc cgacgaaaca gcaaattgtc tgcagccgca gttttggcgt gaagatcacg
     9001 gagtacgagt cactccgtca ggctatctgt cagcacgcag agcgggcctc tgaaaaactg
     9061 cgtaaagagc accagtattg ccggcatatt tccgttttca tcaaaacgtc tccgttcgcc
     9121 attaaagagc cttactacgg caacgtggct acggaaaaac tgcttacgcc cactcaggac
     9181 accagggaca tcattgcagc cgccacaatg gctctggagc gtatctggag ggatgggcac
     9241 cggtatgcca aagccggagt tatgctgaat gacttcaccg gctccggcgt ttcgcagctg
     9301 cagctgtttg acgaacgccc gccacgccct cacagtgctg agctgatgag ggttctggac
     9361 gggatcaaca actcagggct gggtaaagta tggtttgccg gtagaggaat tgcgccatcc
     9421 tggcaaatga aacgcgatat gctctcccct gcgtacacca ctcgctggag agatatcccc
     9481 gttgcgagga tcggttaaat tttgcctttc agagagagcc agcagtgctc gccgcagccg
     9541 aacgacaggg cgaagcccgc gagtgagcga ggaagcacca ggggagctga agccttttaa
     9601 agcaccttac gcacttaagt tctatagatt ccataatgag ttatccacat atcctttgag
     9661 tagatccaat aacttaccct tagttatcca aatagatcca aactgatccc tttagcggca
     9721 aaacctttgt ttacgggctt cgtagcgatt tcaggtgtgt cgtagtggga aaaaggtgtt
     9781 ctgaagcggg aaaaaggtgt gtcaaaacgg ggtaagggtg ggtagaatcg ggaagaaagg
     9841 tgtgtcgaaa cgggaaatag ttaatattta ggcacatatc gatcatatgc tatgaatgga
     9901 tataactata tgaatgagaa tgtttatatc ggcgaggtta taggtccaga ttgtgaaagt
     9961 gttaacaata aactcaccgt cacaagtaag gccacagttc agcctgtagc cctgatgaga
    10021 ttaaatgttt ttgttccttc aagcaggcct gcaaagggca ccagaacatc aatcgatgcg
    10081 acctctgttc tcaaggatct ggaattctca aggcgggagg ggtacgacga tattcgtata
    10141 accggtgaac ggttgagtat ggccatcgac ttcaaggtat gggtcggtat catagaagct
    10201 ttcagtaagt acggtttaaa aaccaacagc attaagctta cttttcatga gtttgctgga
    10261 atgtgccgct ttgagtctaa gagattcgat tcccgtctta gaaaaagtat ttcagaagct
    10321 ttgaccagga tacgtggcaa gaccattacc tttacccgta gcgactcaga gggccttcaa
    10381 aaagtttccg taacaggttt gcttaagaca ggtgaactgg attttgcaaa cgaccaggtg
    10441 gaacttgaag ctgatcagcg actatgggaa ctctaccaaa ctgattacac tgttctgcta
    10501 cgacataaac cgataaacgc tttaacccga aaggaagcag ctcaggccct gtatactttc
    10561 atcgaaagcc tgcctacgaa cccagtcccc atatctttca aacgaatacg agacaggctc
    10621 tcattgctct ctccggtaaa ggaacagaac cgtatcatta aactggccct caagcagttg
    10681 caggatattg ggtaccttga ccacagcatc accaaaaaag gtaaagagac tttcattctg
    10741 atccaccgtc gctcagcaaa tctgaaacca ggaacagctg tataaggtgt gtcaaaacgg
    10801 gaaagcaacg cgattaaggt gtgttgaaac gggaggtctc ttcttaaaca tcttgatagg
    10861 tgtgttgtag ggggaagcct cgccttaaac gtcttaatag gtgtgttgta gcgggatgag
    10921 aacctgttaa atgtgtgttt ttatctgtat atacttacgc accttcccac ttcaacacac
    10981 ctttttggat agaagtgaga agctatcccg cttctcacca ccttttacca aatttgtctc
    11041 accgactccc cacttcgaca caccgaagat tcaaaggctt cccactacgc cccacttttt
    11101 gcttcacgcg gtcataactg gttgatacta ccctccctaa aggtagcctg gagttgaaag
    11161 ccgcattatg tggttctatg gggatttcgg ataaaggcac cactttgcca tcttgttatc
    11221 ttgttatctt gttatcttgc tatcttgcta tcttgctatc ttgctatctt gttatattgt
    11281 tatcttgtta tcctaacatc ttactaattt gttaagatac aaaaacaatt cagttataaa
    11341 tcacacagtt aacaacttaa caagatatta acttaataaa gaaattgcaa tattgtcgcc
    11401 tgtttggcaa tattaagttg ttaacttaac aacttaatat gaggctatga tgataatagt
    11461 attgggtagt caaaagggtg gggttggtaa aagtacactg gcggtatcta tagctgcgta
    11521 tctgatgtca ctgggcaata gagtgatcat tgtcgacgca gatgaccaga aatctgtatt
    11581 gacctggtac aacaatcgtc ctgaagatct tccccatatt ccggttactg gcgcaacagg
    11641 caacatcaaa gccatgctaa aggaacacga gaaatcttat gactttgtca tcgcagactg
    11701 tgctggccgc gacagtgcag aaatgcgtag tgggttgatg gccgctgacg tattcatttc
    11761 tccgttgcgg ccttctcaga tggacctgga tgtagtgcct catacatgct ctgtttttac
    11821 agcagcaaaa gattttaatg aggacgtccg gggatatctc gttttaaaca tgacgcccac
    11881 gaacatgttt gtcaatgaag caaatcaggc agctgacgta ctcaaggatt atcctgaaat
    11941 gcgtctggct agttcccgag tttgtgatcg aaaagcgcat cgagatgcct gggcagaatc
    12001 aatgacaatt tttgaaacgc aaaatgacaa agcacaacaa gaaatcgaat cgttagttaa
    12061 agaggttatt ctgtgagaaa tagagcaaat actccatctc gtgatgaggc agcgtttatt
    12121 aacggtggta ctgctggtat tgataatacc aaagtgactt cagagagacc attggctgta
    12181 agtaaagcaa aaccagtgag catttccttt tttgaagaaa acttaaaaga cattgatgat
    12241 cttataacgg gtgaaatgat ttcaggtaaa agcagagtga atcgttctga cgtagtcagg
    12301 gcagcggtag aggcactaaa aagtttatca aaaacagaga tatccagatt aatcgcagaa
    12361 gcgaaacaac gata
//
LOCUS       DACADF010000136        12102 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.138, whole genome shotgun sequence.
ACCESSION   DACADF010000136 DACADF010000000
VERSION     DACADF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.138"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..637)
                     /locus_tag="ISB30_003754"
     CDS             complement(<1..637)
                     /locus_tag="ISB30_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1991346.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(634..708)
                     /locus_tag="ISB30_003755"
     CDS             complement(634..708)
                     /locus_tag="ISB30_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991347.1"
                     /translation="MKHIQIRNSDMAWHIAANNLGKVS"
     gene            complement(708..1628)
                     /locus_tag="ISB30_003756"
     CDS             complement(708..1628)
                     /locus_tag="ISB30_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1991348.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSLAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKVYVAEVISVLAPFFEETL"
     gene            1766..2665
                     /locus_tag="ISB30_003757"
     CDS             1766..2665
                     /locus_tag="ISB30_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1991349.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            3002..4615
                     /locus_tag="ISB30_003758"
     CDS             3002..4615
                     /locus_tag="ISB30_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1991350.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4666..5697)
                     /gene="ynaI"
                     /locus_tag="ISB30_003759"
     CDS             complement(4666..5697)
                     /gene="ynaI"
                     /locus_tag="ISB30_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAP1991351.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKIKTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPADQGR"
     gene            5941..6198
                     /locus_tag="ISB30_003760"
     CDS             5941..6198
                     /locus_tag="ISB30_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HAP1991352.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(6248..7198)
                     /gene="uspE"
                     /locus_tag="ISB30_003761"
     CDS             complement(6248..7198)
                     /gene="uspE"
                     /locus_tag="ISB30_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAP1991353.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(7350..8102)
                     /locus_tag="ISB30_003762"
     CDS             complement(7350..8102)
                     /locus_tag="ISB30_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAP1991354.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(8297..8812)
                     /gene="ogt"
                     /locus_tag="ISB30_003763"
     CDS             complement(8297..8812)
                     /gene="ogt"
                     /locus_tag="ISB30_003763"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP1991355.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(8823..8966)
                     /locus_tag="ISB30_003764"
                     /pseudo
     CDS             complement(8823..8966)
                     /locus_tag="ISB30_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9355..10170)
                     /locus_tag="ISB30_003765"
     CDS             complement(9355..10170)
                     /locus_tag="ISB30_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1991356.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            10267..11295
                     /locus_tag="ISB30_003766"
     CDS             10267..11295
                     /locus_tag="ISB30_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP1991357.1"
                     /translation="MIISKKQLIGVVAIGILLAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVTSKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLES
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            11292..>12102
                     /locus_tag="ISB30_003767"
     CDS             11292..>12102
                     /locus_tag="ISB30_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAP1991358.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMIFF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTIFLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCF"
BASE COUNT         3224 a         2700 c         2842 g         3336 t
ORIGIN      
        1 acatgaagaa gggcttatca ccatcagtcc cgggttgatt gatgcctata ttctcaccaa
       61 tgtgtttctc aggtttatcg atgatatctg tgattaatgc tgcgacactc ttacgtgata
      121 cgatcgtacc tttgaatggt tcattgcgtg aggtaagctc ataatcaata atatcttcat
      181 cagtcagcca ggcagggcga agaattgtat attccagtcc agaagcctca attgcatccg
      241 cagccctgcg gaatggtttt aggggttcac caataactgc attattccat tcgccaaatt
      301 ttcctggaac ttcatcataa ataccgaggg aaagaacaaa gatcagacgt ttaacatcac
      361 aggctttcat cgcagcgatc actgaatttg cctgaatatc taaatcttcc cccgtaaggt
      421 tggcatacac aacatcctgt ccctgcatgg cctgcttcag tgctgcatga ttcagtacat
      481 cccccataat aatcttgcta ttagttggat agggtttgtg gatttttgct ggctgacggg
      541 caaataaagt ttgtttaatg gtttgtttat ctgccagttg attaatcaca tggcgggcta
      601 tctgaccgcc agcaccaaga atcagtacat ttttcatgaa acttttccca gattattggc
      661 agcaatatgc caggccatat cactattacg gatttgaata tgtttcatta cagagtttcc
      721 tcaaagaacg gagctaatac tgaaattact tcagccacat agactttgcc atcataaaga
      781 tccatatggt ttgcgccttc aacaatgtga tagcgtttat cctggcttga tgctcgatcg
      841 tacaggtcgt cactcatcca tttgctccca gccaggctgc ccgccacaat ctgtatcggc
      901 tgagtcaggt acacttccgc catatggtaa gcatcatagg taataatctg gttaaggctg
      961 cgcaaagtag cgtaacccgg tgctgttgga tactgcgcgc gaggggtgtg gtaatattcc
     1021 caggcctgac gcagttcttc gttcggcgca tcggactcct tcattggtgc cagtggcata
     1081 gtggcgtatt ctccgctgct aatatcgctg gttcgggcgt ttgaacccgc gtcaacgtat
     1141 ggtagggcat caatagattt cacattgttt tcccaaccat tacggaacat cgaaccaata
     1201 ttgaccgcac taacagtacc gatggccttg atgcggcgat cctgaattgc agcattggct
     1261 gtatatcctg caccggcaca aattcccatc gcaccaattc gggtattgtc gacataagaa
     1321 agcgttgtca ggtaatcaat cacggcactg acgtcttcag tacgaatgta tgggttttct
     1381 aactgacgcg gctcgccgcc gctttcacct tgataagatg cgtcataagc aatagtgaca
     1441 aatccttttt ccgccagttt ttcggcatag gttccggccg tttgctcttt aacgccccca
     1501 cctgggtgag ataccacaat tgccggatac tgacgggttt catcaaattt tggagggaaa
     1561 tagatcactg cagacaaaga gatggttgga ttattgctgt tagtgaagct gactttatta
     1621 ttcatcattc cgttcctcat ggagttgtcg gttcgtttta acggttggtg atatcactat
     1681 agatattgat cattaagttg attagaccca aaatcatgat tagacctatc atttaaatga
     1741 ttaatagatt gtatggagat actgaatgaa gcgtgaagaa atcgctgatc tgatggcgtt
     1801 tgtcgttgtt gcagaggagc gtagcttcac tcgcgcagca gcccgcctga gcatggcgca
     1861 gtcagcttta agccagatag tgcgccgtat agaagaacga ttgggattgc ggcttctgac
     1921 gcgaaccacg cgcagcgttg tacccactga agcgggcgag catcttttgt ctgttcttgg
     1981 ccctatgttg catgacatag attctgccct gacatccctg agcgatctgc agaaccgccc
     2041 atccgggaca atacgtatta ctactgtaga acatgcagca aaaacgatat tgttaccagc
     2101 catgcgcaca ttcctgaaat cgcatcctga aattgatatt cagcttacca ttgattatgg
     2161 tttgaccgat gtcgtttctg aacgttttga tgcaggcgtc cgtctgggtg gggagatgga
     2221 taaagatatg atcgccattc gaatcgggcc agacatacca atggctattg ttggctcacc
     2281 ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca caattaatag atcatcaggc
     2341 aattaatttg tatcttccca catcgggtac agcaaatcgc tggagattaa tacgcggtgg
     2401 acgtgaagtt cgtgttcgca tggaaggtca gcttttactg aatacgatag atctgatcat
     2461 tgatgctgca attgatgggc atggattggc gtatctacct tatgatcagg tggagcgggc
     2521 tattaaagaa aaaaaactga tacgtgtttt ggataaattc acaccagatt tacccggtta
     2581 tcacctgtac tatccacacc gtcgacatgc tggctcggca ttctcattat ttatagatag
     2641 gctgaagtat aaaggtgctg tttagcacta cttgctgata cattaattta attcttctct
     2701 taacgtattc tcagttcctt tcaacgtttt ggttattttt tattcttcgc acaatggcga
     2761 cagatgctga ttatgatacc gaaaacgggt ttgaatgtgc gaagcccgaa cgtagtgttc
     2821 ggagttctat gtgctttacc gcattttgga gactattatt tacactaaat ctgatttgat
     2881 atattgatac ttaaaacatt tgatgcttcc tttgtcactt ttttgatgga agttgtttgc
     2941 atttctttaa ggcgaaacaa ataattacgc atcaatttta atgtcggtta gagggaaact
     3001 tatgaagcac tctgtttcag tcacgtgttg tgcgctgttg gtcagcagca tttctctttc
     3061 gtatgctgct gaagttccga gcggcacagt actggcagag aagcaggagc tggtgcgcca
     3121 cattaaagat gagccagcgt cgctggatcc cgctaaagcc gtgggcctgc cggagattca
     3181 ggtcattcgc gatctgtttg aaggactggt gaatcagaac gagaaagggg agattgtccc
     3241 cggcgttgcg actcagtgga aaagtaatga caaccgtatc tggactttta ccctgcgcga
     3301 taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa gattttgtct acagctggca
     3361 acgtctggtg gacccgaaaa cgctttcgcc atttgcatgg tttgccgcgc tggcgggaat
     3421 caacaacgca caggcgatta ttgatggtaa agccacgcct gaccagcttg gcgttaccgc
     3481 agttgatgcc catactttga aaatccagct tgataaaccg ttgccgtggt ttgtgaattt
     3541 aaccgctaac tttgccttct tcccggtgca aaaagccaac gtagaaagca gtaaagagtg
     3601 gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt cttaaagagc gcgtagtcaa
     3661 tgaaaaactg gtcgtagtgc cgaataccca ttattgggat aacgccaaaa cggtactgca
     3721 aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc actaagcgtt atctcgcggg
     3781 ggatattgat atcaccgaat ccttcccgaa aaatatgtat cagaagctgt tgaaggatat
     3841 tccggggcag gtttatacgc cgccgcagct cgggacctat tattatgcgt ttaacacgca
     3901 aaaagggccg acggcagatc agcgcgttcg cctggcatta agtatgacga tagatcgccg
     3961 cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca gcgtggcatt ttacaccaga
     4021 tgttaccgcg ggatttacgc cggaaccttc gccgtttgaa caaatgagtc aggaagaact
     4081 gaatgcgcag gcaaaaactt tgttgagcgc cgctggttat ggtccgcaaa aaccgctgaa
     4141 gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa attgcgattg ctgtagcatc
     4201 gatgtggaaa aagaacctgg gcgtagatgt taaattgcaa aaccaggaat ggaaaaccta
     4261 tatcgatagc cgtaataccg gcaattttga tgttatccgc gcctcgtggg taggggatta
     4321 taatgaaccc tccactttcc tgacattatt aacgtcaacg cattccggga atatttcacg
     4381 ctttaacaat ccggcatatg acaaagttct ggcccaggca tcgacggaaa ataccgttaa
     4441 agcgcgtaat gccgattaca acgcggcaga aaaaatcctc atggagcaag caccgattgc
     4501 accaatttat caatatacca atggacgatt aatcaagccg tggctgaaag gttatcccat
     4561 taacaaccct gaagatgtgg cgtacagtcg gactatgtat attgtgaagc attgatgtga
     4621 tgagaactgg cgttaccctt gtggataacg ccagtgatgt ctgatttagc gaccctggtc
     4681 cgccggtgta atgttatcca tatacagcgt ctggctgggg aaggcaaagt ccgcgccgtg
     4741 tgactgtaca atatcgataa tcttcaaata aacgtcttgc tgtgcagcaa gccattcagc
     4801 ccataccgtg gttttggtaa agcaataaac cataatattt aatgacgaat cagcaaactg
     4861 gttgaaataa accagtaagg tttgtcgctg gtcgatggcc gggtgatttt tcagcatctc
     4921 acgtacggct tcgacaataa cgcccacttt tgccgcatcc tcataacgta aaccaatggt
     4981 cgtggtaatg cgccggttgg tcattcgtcc aggattttct acgctgatcg acgaaaacag
     5041 cgagttcggt acgtacaatg gacgattatc aaaggtctta attttggtaa ttcgccagcc
     5101 aatttccgct actgtacctt cgatatttct gtccggtgaa cggatccagt cgccaatact
     5161 gaaaggacgg tcgaaataga gcataatccc ggaaaagaag ttactcagaa tatctttacc
     5221 ggccataccg acagccagac caccaatacc accaaaggtc aacaagccag aaaggctcat
     5281 gccgaaatgt tcgccataaa gcagaacaag caccacaata atggtgattt tgataatgcg
     5341 cgacatgatc cgcgcactgg tgatatcgcg acctttttta atctgctgtt tttcaaactg
     5401 attaatcagc agaaatagct taatcgtcag aataaccgca atcagggacg tacagataaa
     5461 atcgataacg cctggggtga taaatttgag tttatagttt tctataacat agttaatgat
     5521 gctaccaaca gcactaataa ttatggtgta gattaaaaat tgcaccgcat ggaataaaaa
     5581 tccttttctt ttacgatttc cacggcgaaa ccaaaagctc atcagaatca atgctgcgca
     5641 gctaccgaaa ataatgacca gattaagcgc attatttgta aacagttcag cgatcattgt
     5701 tttatcaggc tcctccagat aattgtcgtc atgccggaaa cccctggcgg gactatttta
     5761 ccgcgacaat tcattcagat catcaatagt cagggaagga agtagcaaca ttagctaact
     5821 aatcaatgcg ttgattgtaa atccagctaa gaggtgaggt tttcagagca gacaacggtg
     5881 aaatgtcatg gtattgttac gtttaggtaa caaaaaattt gtctgcacaa ggattacatc
     5941 atgattatgg cgaaactgaa gtcagcgaaa gggaagaaat ttctctttgg tttgttggcg
     6001 gttttcatta ttgcggcgtc ggttgtgact cgcgcgacca tcggcggcgt tatagaacag
     6061 tacaatattc cgctgtctga gtggacgaca tcaatgtatg tgattcagtc atcgatgatt
     6121 tttgtttata gcctggtctt tactgtgttg ctggcaatcc cgttgggaat ttatttcctt
     6181 ggcggcgaag agcagtaagt aaaaaatagg cccgataact cgggccttgt cagttattga
     6241 agagtcgtta atcgtcttct tcgtcatcca gttcaacggg tgtctgatac tggtcaggtt
     6301 taatgaccag caggtcgcag cgaagatgat caatcacctg ttccgccgtg ttgccgagga
     6361 atgctgctga aataccggtg cgtccgaccg tgcccagaac cacaatcccc gcctgtaagt
     6421 gctccgccaa atcaggaatc acctcttctg gcagaccttt ttctacgtgc gtcatgtttt
     6481 cattaatgcc gaatttctgt cgcagagctt tcattgccag caaatgttgc ccacgaatgg
     6541 catcgttata aacgctcggg tcaaattccg gcagttcaat cgcgatatta attggcgtta
     6601 ccggataagc gccaaccaga tgaacttcgg tatggttgac ttgttctgcc agttcgatcg
     6661 tctctttgac cagtttttca ttgagcgcat tatggtacgg ctcttcactg gcgagattca
     6721 ccgccaccag cgccttaccc ccttccggcc acggctggtc tttcaccatc cacacggggc
     6781 ttgggcattt gcgtaacaga tgccagtccg ttggcgtaaa aatcaccgct tccagacggt
     6841 catgttggtg cgccattttt agcaccaaat cgtgcccgcc gctgatcact tcctgaatga
     6901 tggcttcgaa aggacggtta tgccagacca ctttaatttc aatgggaacg ccagcattga
     6961 gataatattt tgcctgctcg tggatccagg ctgtacgctg gctgatgacg ccctgacgca
     7021 tagcggtacg ttcgtccgga gagagcaggg tggtcatttc gtatgagaag tcatagatcg
     7081 gcaaaaaggc tttaattttg ccaccaatcc gttgatgtaa ataaacagct cgccgcaatg
     7141 ctggttggtc gtcctggtta ggatcgataa caacgagcat gttctgatac atagccatac
     7201 agggtctcct tacaacaact gtcaacgcag tttgtaatta aaagattaac ccatatctgg
     7261 tgaatgaaac agtgatgaac cttctgtcag atcaataaat cagaaaaatt taatgatatg
     7321 acagaaggat agtgagttat gcggaagaat caggcaacgt tacgcgtgtg accagcaagc
     7381 tgggccagcg catcgttatt ttcgatagtg atgtatttac ctttgactgc cagcataccg
     7441 cttttctgga agcgacccag cagacggctg atggtttcaa ccgtcaggcc cagatagtta
     7501 ccgatatcac cacgagtcat cgtcaggcgg aattcacgag gggagaagcc gcgttgggca
     7561 aaacgacggg acaggttgta gatgaatgca gccagacgtt cctcggcatt tttcttcgac
     7621 aacagcagga tcatgtcctg atcgcctttg atttcaccgc tcatcagacg catcatctgc
     7681 tgacgcagat tcggcatttt accagacaaa tcgtccagcg tttcgaacgg gatttcacat
     7741 accatcgagg tttccagcgc ctgcgcgaaa ctcgggtgat gaccgctgcc gatggcatca
     7801 aatcccacca gatcgcctgc taaatggaaa ccagtgattt gctcgtcgcc ttgctcagtg
     7861 atggtataac ttttaatcgt accggagcgg atggcataaa gcgatttaag ttcatctcca
     7921 gccttaaaca gcgtctggcc tttctgaata ggcttcttcc gctcaatgat attatcaagc
     7981 tgatcaagct catgttcgtt gagtgtgaac gggatgcaaa gctggctgat gctgcaatcc
     8041 tggcaatgga tagcacaacc gccagactga atgcgccgta taattcgctt ttccgggatc
     8101 ataggtctgc tcaagccgta attgatattt gtcaatttta acatcttttt agggagcaag
     8161 taagtctaag caaaccttaa cagcagagaa ttccgatatt agatgtaaat atatgtctat
     8221 ctatttgaaa acccttaagt tgttaagggt gactttacat aaaagtgtga acaagctggc
     8281 acaaattgtt taatgtttac agcaaaagat aaccttcgtg gcgcaataac cactcttttc
     8341 gctgaactcc gcctgcatat ccggtcatgg tgccgtttcg gccaataacc cgatggcaag
     8401 gtacgacgat gctgatggga ttcgatccgt ttgccgcacc aacggcacgc gccgcgccag
     8461 ggcggcctaa ttgttcagcc agttggccat aatgcattac ctgcccgcag gggatagtgc
     8521 gcagtgtttt ccagacttcg cgctgaaatg gcgtcccccc cgtggcagtg ggaagcgtat
     8581 caataatgct aagattaccg gcaaaatatt cacgcagctt gtcgcttaaa ccacctgggt
     8641 tggtggcaga aatgcgctca tagccttctt tgcgataatg gatgtccagc agctgcacca
     8701 tgcgttcgct gtactcttcc cattcaaccg cccgcaggcg aaattgctca tcgcaaatca
     8761 cccacagtgg acccagtggc gtggcaattt tttcttcaag taatctcagc atccgttctc
     8821 tcttaagaca aacgtgggta aatacccgga cctatcggca agccaacaag ataccacgcc
     8881 agcaacatca gcagccatac caccaaaaag ataagtggat agggtaagac taacgaatag
     8941 taagtaccca gtttcgcgtc tggtttgtcg cagtctgata gtattaagcg attttttgat
     9001 ttttctgcct gaagaaatga gggcaagcca tcacatggat cgagggtggg ataattccag
     9061 tcagcgaaat tttaccgtag gttatctgtt atttgatctg tggcgtgata gccgccagac
     9121 accactgatc tcgtcactca ttccccaatc ccggcgagaa tgtggttatt tacagggcta
     9181 aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt acacacagca
     9241 gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt gttataacgg
     9301 ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata taagttaaat
     9361 catttgtctt gtgattgcat aatcatgcat cgttaatcga cgagtaaatt taccgggcga
     9421 gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat agcccagacg
     9481 atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag aaataacgtg
     9541 catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag
     9601 tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg
     9661 gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc
     9721 aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa
     9781 acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag
     9841 taattccctt aacaggggtg aaatacccgt catgaagcaa acttttccca gcgtttcact
     9901 ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg
     9961 cggcatttgt gggggcagcc aaagcgcgct gctggcatgc actagccaga cgctttctcc
    10021 agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg
    10081 ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg
    10141 tgatgctttg ggttgcagtg tgattgtcat ttacagtagg tattttgtca gaaatcatca
    10201 aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa
    10261 agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg
    10321 gctggcgttg tcttttttat ctggtgggta agcaaaggaa gatttataca gacgacggat
    10381 gatgcttaca ttggtggtaa tatcaccacg gtgacaagta aagtttctgg ttatatttct
    10441 gctatagagg tgcgcgataa tcagagtgta aaaaaaggcg atattatcct gcgtctcgat
    10501 gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag tagcaaagcc
    10561 aatctcgaaa gcattcaagc cactattgcg atgcagcagt cgataattca aagtgccagt
    10621 gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac tgaacgttat
    10681 gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc caggtttgac
    10741 taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt ggagaagcaa
    10801 cggctggcag ttttatctgc tcaggaagaa aacgtacgtg caagtatcga agaagttcag
    10861 gccgctttga ctcaggcact actggatctg gaatataccc ttgttcgtgc gccgattgat
    10921 ggcattgtcg caaaccgcag cgcccataca ggcagttggg tggaaggcgg cacatcgtta
    10981 gtttcactag taccagtcag tgagttgtgg gttgatgcaa attataaaga aaaccaaata
    11041 gcgggaatga agccaggaat gaaggctgag atccgtgctg atattctcaa aggggaagtg
    11101 tttcacggtc acattgaaag cctttcccca gcgacaggag ccagtttcag cttgattccg
    11161 atagaaaatg ccactggcaa tttcaccaaa attgttcagc gtgtcccggt acggattgca
    11221 tttgacgacg caaaggaatt aaagcaactt cttcgcccgg gactgtccgt caccgtcagt
    11281 gttgatgaga gatgatgtct atgcgtaaac atattgcttt cgccagtatg tgtatgggat
    11341 tatttattgc tcagcttgat attcaaattg tatcgtcatc cctaaatgaa ataggtggtg
    11401 gtctgtccgc aggtaaagac gaaatggcct ggcttcagac cagctattta attgctgaga
    11461 ttattgttat tccgctctct gggtggctgt cacgggtatt ttccactcgt tggcttttta
    11521 ctctatccgc gggaatattt actttaatga gtatcgcgtg tggacttgct tggaatattc
    11581 agatcatgat tttttttcgt gctttacagg gagctgctgg cgcatccatg atccctttag
    11641 ttttcacgac ggcgtttatt tattaccagg gtaaagagtt ggggttggct gctgcggtag
    11701 tcagtgcgct ggcctcactg tcaccgacgc tagggccaac tctggggggc tggatcactg
    11761 ataacctcga ctggcgatgg ctattttata tcaatatatt acctggcata tatttagttt
    11821 tgagtattcc ttttctggtt aattttgata aacctgatct ctctttactg aaagttgctg
    11881 attatccgag tattatcctg ttggccatga cactgggatg tctggaatat acccttgaag
    11941 aaggagcgcg ctggggatgg ctggatgata atactatttt tctgacatca gttctggcat
    12001 tggtgagttt tattttattt gctgcgcgta cacttacaat atccaatccg ataatggatc
    12061 ttcatgcttt taaggataaa aatttcacac tgggatgttt tt
//
LOCUS       DACADF010000137        12095 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.139, whole genome shotgun sequence.
ACCESSION   DACADF010000137 DACADF010000000
VERSION     DACADF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.139"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            87..956
                     /locus_tag="ISB30_003768"
     CDS             87..956
                     /locus_tag="ISB30_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991359.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDDGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1006..1359)
                     /locus_tag="ISB30_003769"
     CDS             complement(1006..1359)
                     /locus_tag="ISB30_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAP1991360.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1497..3143)
                     /gene="groL"
                     /locus_tag="ISB30_003770"
     CDS             complement(1497..3143)
                     /gene="groL"
                     /locus_tag="ISB30_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP1991361.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3187..3480)
                     /locus_tag="ISB30_003771"
     CDS             complement(3187..3480)
                     /locus_tag="ISB30_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP1991362.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3756..5012
                     /gene="yjeH"
                     /locus_tag="ISB30_003772"
     CDS             3756..5012
                     /gene="yjeH"
                     /locus_tag="ISB30_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAP1991363.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5028..5504)
                     /gene="fxsA"
                     /locus_tag="ISB30_003773"
     CDS             complement(5028..5504)
                     /gene="fxsA"
                     /locus_tag="ISB30_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAP1991364.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5841..7277
                     /gene="aspA"
                     /locus_tag="ISB30_003774"
     CDS             5841..7277
                     /gene="aspA"
                     /locus_tag="ISB30_003774"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAP1991365.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7395..8696
                     /locus_tag="ISB30_003775"
     CDS             7395..8696
                     /locus_tag="ISB30_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAP1991366.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8812..9150
                     /gene="cutA"
                     /locus_tag="ISB30_003776"
     CDS             8812..9150
                     /gene="cutA"
                     /locus_tag="ISB30_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAP1991367.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9126..10823
                     /locus_tag="ISB30_003777"
     CDS             9126..10823
                     /locus_tag="ISB30_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAP1991368.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDSQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10860..11435
                     /locus_tag="ISB30_003778"
     CDS             10860..11435
                     /locus_tag="ISB30_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1991369.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11542..11617
                     /locus_tag="ISB30_003779"
     tRNA            11542..11617
                     /locus_tag="ISB30_003779"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11575..11577,aa:Phe,seq:gaa)
BASE COUNT         2932 a         3107 c         2952 g         3104 t
ORIGIN      
        1 ttaacaacta ttactaccaa gcccatttat ctggcttata aactaaaatt aattatttaa
       61 ttgagaaatg gttagggaga acctacatgg ccataagcat caagggtgtt aataccggtg
      121 ttattcgcaa gagtaataat ttcattgcac tggcgctgaa aattaaagaa cctcgcaata
      181 aagaatcgtt gttctttatg tctgccatgg aattgcgcga ccttctgatt gcgctggaaa
      241 gtcgtctgca tcaaaagcat aaactggatg ctgctgcacg ccttcagtat gaacaagctc
      301 gcgataaagt cattaagaaa atggcagaaa atatcccaga aattctggtt gatgagctta
      361 aaaatgccga tattaatcgt cgggttaaca cgctggaact taccgatgat cagggcgaaa
      421 atcttacctt cgtattaaca ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt
      481 tgcagattga aatgctggcg cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg
      541 aactggcatt acgtattacc tcactgttag atttcctgcc gttatatgat gtcgactgcc
      601 aggatgatgg taatctggaa tatgacacct attcccaacc ggaatggaaa cataacttgt
      661 tcaaccatta tctggcggtg ctttaccgct ttaaagatga aagtggcaaa gaacaattta
      721 gcggcgcagt ggtgaaaacg cgtgaagcaa cgccgggtaa agaagtggag gcgatcactc
      781 gccgaatgct tgatttcagc ccccgactga aaaaactcgc gggtgtgcca tgtcaggtct
      841 atgttcgcac cgtagcggca aataacgccc aaccgctaac ccaggatcaa tgcttacgcg
      901 cgctgcatca cttacgtgtt caatccacca gcaaaactgc accacaggcg aaataatctt
      961 atctgtttta ctacgaggct ggaagtatcc agcctctgga tcacatcagt tcgggcactt
     1021 ataaacctga ccgataatct ggctatccgt cgggacaaaa ctggacaaca ttccctgcga
     1081 cgggctgctg atgccataaa tcacgttacc gcccattgca gccgcctggt tgcgcagatc
     1141 gtttgctgcg ccgcgcatag aaccgccttc ttctccgtgt tgcccggaaa gccagttgct
     1201 ttgcttacct gttgcagtac caatcagctg gcactctgca cctggttgct cgtccacaat
     1261 gcgtacactc tgaccggcag cactcaattc gttgctggag ctacaacccg ccatcagtag
     1321 cgcggcaccg acaatccctg ctaagtattt tacgtgcatg ttattcccca taatcaatga
     1381 gctggacgcc actcgcgtcg tccgtgtctg aatcttatac tagaaagatg accaaaagaa
     1441 aaacccccag acatttctgc ccgggggttt gtttatttct gcgaggtgca gggcaattac
     1501 atcatgccgc ccatgccacc catgccgccc ataccgccag cagcgcctaa gtcagctgca
     1561 tcgtttttcg gcaggtcggt aaccatgcac tcggtggtga tcatcaggcc agccacagaa
     1621 gccgcgtact gcagagcaga acgggttact ttggttggat ccaggatacc catatcgatc
     1681 atgttgccgt attcttcagt tgctgcgttg taaccgtagt tgccgtcgcc gcctttaacg
     1741 gtgttagcaa caacagacgg ttcttcgccg cagttcagga cgatctgacg cagcggagct
     1801 tccattgcac gcagtgcaac tttgataccc acgttctggt cttcgttctg accacgcagg
     1861 tcagccagtt tagacgctac gcggatcagc gcaacaccac caccagcaac cacgccttct
     1921 tctaccgcag cacgggtcgc gtgcagggcg tcttcaacgc gtgctttttt ctctttcatt
     1981 tcaacttcgg tagcagcacc aactttgata actgcaacgc cgcctgccag tttcgctacg
     2041 cgctcctgca gtttttcacg gtcgtagtca gaagttgctt cttcaatctg ctgacggatc
     2101 tgagcaacac ggccctggat tgcagcttct tcacccacgc catcgatgat ggtggtggtg
     2161 tctttgttga tcacaacacg tttagcctga cccaggtctt ccagggttgc tttttccagc
     2221 tccataccga tctcttcaga gattacggta ccgccagtca gggttgcgat atcctgcagc
     2281 atagctttac gacgatcgcc gaagcccgga gctttaaccg cagcaacttt cacgatgcca
     2341 cgcatggtgt taacaaccag agttgccagt gcttcgcctt caacatcttc agcgatgatc
     2401 agcagcggtt tacctgcttt ggcaacagct tccagaaccg gcagcatttc gcggatgttg
     2461 gagattttct tgtcagccag caggatgaac gggctttcca gttctactgc gccagtttcc
     2521 ggcttgttga tgaagtaagg agacaagtag ccacggtcga actgcatacc ttcaaccacg
     2581 tccagttcgt cctgcagacc ggtaccgtct tcaacggtga taacgccttc tttaccgact
     2641 ttgtccatcg cttcagcgat cagtttacct acggtttcgt cggagttagc agagatggta
     2701 ccaacctgag caatcgcttt agagtcagag cacggtacgg acagcgcttt cagttcttca
     2761 actgcagcgg taacagcttt gtcgatacca cgtttcaggt ccatcgggtt catgcccgca
     2821 gcaacagctt tcagaccctc agtgatgata gcctgagcca gtacggttgc agtggtggta
     2881 ccgtcgcctg cagcgtcatt cgctttagag gcaacttctt tcaccatctg cgcacccata
     2941 ttttcgaact tgtcttccag ttcgatttca cgagcaacgg aaacaccatc tttggtgatg
     3001 gtcggtgcac cgaaagattt atccagaact acgttacggc ctttaggacc gagggtaact
     3061 ttcactgcat ctgccagtac gtttacgccg cgcagcattt tcacacgagc gtcgttaccg
     3121 aattttacgt ctttagctgc cattatcttt attccttaaa ttcgtatgtt cagtgtcgtg
     3181 cgcggattac gcttcaacaa ttgccagaat gtcgctttcg gacatgatca acacttcttc
     3241 attgtcgatc ttctcagatt tcacaccgta gccatcgttg aaaataacga tgtcgccaac
     3301 tttcacatcc agcggcttca cttcgccatt ttcaaggata cggccattgc cgacagccag
     3361 cacttcgccg cgggtggatt tagccgctgc agagccggtc agaacgatgc cgccagcaga
     3421 tttagtttca acttctttac gcttgacgat cacgcgatca tgcaatggac gaatattcat
     3481 tgataactct cctttgagaa agtccgtatc tgttatgggt gacgccggag cttacgtggt
     3541 ttcccggctg gtgaccagag aaatggggat gaggcttcgc cccttcaagg gggaaaaaga
     3601 aaaaaaattc tgatcagcac aaaatcgggt gaaaaccctg attcacctca catttcatcg
     3661 ctatttcttc atcaccgtca taagcaaatc tgattgtgct accatcgaaa atctacgcag
     3721 ttgcccaaaa tttgggcgca atcggacatc aacccatgag tggactcaaa caagaactgg
     3781 ggctggccca gggcatcggc ctgctatcga cgtcattatt aggcactggc gtgtttgccg
     3841 ttcctgcgtt agctgcgcta gtagcaggca ataacagcct gtgggcgtgg cccgttttga
     3901 ttatcttagt gttcccgatt gcgattgtgt ttgcgattct gggtcgccac tatcccagcg
     3961 caggcggcgt cgcgcacttc gtcggtatgg cgtttggttc gcggcttgag cgagtcaccg
     4021 gctggttgtt tttatcggtc attcccgtgg gtttgcctgc cgcgctacaa attgctgccg
     4081 gattcggtca ggcaatgttt ggctggcata gctggcaact gttgttggca gaactcggta
     4141 cgctggcgct ggtgtggtat atcggtactc gcggtgccag ttccagtgct aatctacaaa
     4201 cagttattgc cggacttatc gtcgcactga ttgtcgctat ctggtgggcg ggcgatatca
     4261 aacctgcgaa tatccccttc cctgcgccag gaaatatcga acttaccggg ttatttgctg
     4321 cgttatcagt gatgttctgg tgttttgtcg gtctggaagc atttgcccat cttgcctcgg
     4381 aatttaaaaa tccagagcgt gattttcctc gtgctttgat gattggcctg ctgctggcag
     4441 gattagtcta ttggggctgt acggtagtcg tcttacactt cgacgcctat ggtgaacaaa
     4501 tggcggcggc agcatcgctt cccaaaattg tagtgcagtt gttcggtgta ggagcgttat
     4561 ggattgcctg cgtaattggc tatctggcct gctttgccag tctcaacatt tatatacaga
     4621 gcttcgcccg cctggtctgg tcgcaggcgc aacataatcc tgaccactac ctggcacgcc
     4681 tctcttctcg ccatatcccg aataatgccc tcaatgcggt gctcggctgc tgcgtggtaa
     4741 gcacgttggt gattcatgct ttagagatca atctggacgc tcttattatt tatgccaatg
     4801 gcatctttat tatgatttat ctgttatgca tgctggcagg ctgtaaatta ttgcaaggac
     4861 gttatcgact actggcggtg gttggcgggc tattatgcgt tctgttactg gcaatggtcg
     4921 gctggaaaag tctctacgcg ctgatcatgc tggcggggtt atggctgttt ctgccaaaac
     4981 gaaaaacgcc ggaaaatggc ataaccacat aatccggcgt ttcgacatta atcctggcga
     5041 tcgtctttat gatcaaggcg gtcgcggtca tcatcctttc gctggtactc accatcaaaa
     5101 gtattaccgc caccggtccc ggcgctaaaa ccgccgcctg gcatgcgaga aaagcgcaaa
     5161 tgcggcatca acttcactgt cagatgcttt tgcaccggcg gcaataaaag cagaagaccg
     5221 aggaagtcgg tgaaaaagcc cggtaataaa agcagcaaac cggcaatgat cagcgaaaca
     5281 cttttaatca tctccgccgc tgggttttca cccgccgcca ttttttgctg catcagcacg
     5341 aaattcttaa agccctggtt acgtaccagt gacataccga taactgacgt gaatataacc
     5401 agcacgaggg tcagcaatac ccccaataca tgagcaacct gaataaagat tgaaatctca
     5461 atatagacat aaaggaaaat ggcaataaaa ggtaaccagc gcaaaggttt ctcctgtaat
     5521 agcagccggt taaacccggc tacctgaatg ggttgcgaat cgcgtttagc ttatattgtg
     5581 gtcattagca aaatttcaag atgtttgcgc aactattttt ggtagtaatc ccaaagcggt
     5641 gatccatttc acaaattaat aattaagggg taaaaaccga cacttaaagt gatccaggtt
     5701 acggtagaaa tcctcaagca gcatatgatc tcgggtattc ggtcgatgca ggggataatc
     5761 gtcggtcgaa aaacattcga aaccacatat attctgtgtg tttaaagcaa atcattggca
     5821 gcttgaaaaa gaaggttcac atgtcaaaca acattcgtat cgaagaagat ctgttgggta
     5881 ccagggaagt tccagctgat gcctactatg gtgttcacac tctgagagcg attgaaaact
     5941 tctatatcag caacaacaaa atcagtgata ttcctgaatt tgttcgcggt atggtaatgg
     6001 ttaaaaaagc cgcagctatg gcaaacaaag agctgcaaac cattcctaaa agtgtagcga
     6061 atgccatcat tgccgcatgt gatgaagtcc tgaacaacgg aaaatgcatg gatcagttcc
     6121 cggtagacgt ctaccagggc ggcgcaggta cttccgtaaa catgaacacc aacgaagtgc
     6181 tggccaatat cggtctggaa ctgatgggtc accagaaagg tgaatatcag tacctgaacc
     6241 cgaacgacca tgttaacaaa tgtcagtcta ctaacgacgc ctacccgacc ggtttccgta
     6301 tcgcagttta ctcttccctg attaaactgg tagatgcgat taaccaactg cgtgaaggct
     6361 ttgaacgtaa agctgtcgaa ttccaggaca tcctgaaaat gggtcgtacc cagctgcagg
     6421 acgcagtacc gatgaccctc ggtcaggaat tccgcgcttt cagcatcctg ctgaaagaag
     6481 aagtgaaaaa catccaacgt accgctgaac tgctgctgga agttaacctt ggcgcaacag
     6541 caatcggtac tggtctgaac acgccgaaag agtactctcc gctggcagtg aaaaaactgg
     6601 ctgaagtcac tggcttccca tgcgtaccgg ctgaagacct gatcgaagcg acctccgact
     6661 gcggcgctta tgttatggtt cacggcgcgc tgaaacgcct ggctgtgaag atgtccaaaa
     6721 tctgtaacga cctgcgcttg ctctcttctg gtccacgtgc cggcctgaac gagatcaacc
     6781 tgccggaact gcaggcaggc tcttccatca tgccagctaa agtaaacccg gttgttccgg
     6841 aagtggttaa ccaggtatgc ttcaaagtca tcggtaacga caccactgtt accatggcag
     6901 cagaagcagg tcagctgcag ttgaacgtta tggagccagt cattggccag gccatgttcg
     6961 aatccgttca cattctgacc aacgcttgct acaacctgct ggaaaaatgc attaacggca
     7021 tcactgctaa caaagaagtg tgcgaaggtt acgtttacaa ctctatcggt atcgttactt
     7081 acctgaaccc gttcatcggt caccacaacg gtgacatcgt gggtaaaatc tgtgccgaaa
     7141 ccggtaagag tgtacgtgaa gtcgttctgg aacgcggtct gttgactgaa gcggaacttg
     7201 acgatatttt ctccgtacag aatctgatgc acccggctta caaagcaaaa cgctatactg
     7261 atgaaagcga acagtaatcg tacagggtag tacaaataaa gaaggcacgt cagatggcgt
     7321 gccttttttc ttgtgagcag taacttaaaa ataacaattt aatatcaact tgttaaaaaa
     7381 caaggaaggc taatatgcta gttgtagaac tcatcatagt tttgctggcg atcttcttgg
     7441 gcgccagatt ggggggaata ggtattggtt ttgcaggcgg attgggggtg ctggttcttg
     7501 ccgctattgg cgttaaaccc ggtaacatcc cgttcgatgt catttccatt atcatggcgg
     7561 ttatcgccgc tatttctgcc atgcaggttg ctggcggtct ggactatctg gttcatcaga
     7621 cagaaaagct gctgcgccgt aacccgaaat acatcacgat cctcgcaccg atcgtgacct
     7681 atttcctgac tatctttgct ggtactggca acatctctct ggcgacactg ccagttatcg
     7741 ctgaagttgc gaaggaacaa ggcgttaaac cttgccgtcc gctgtctact gcagtggtat
     7801 ccgcgcagat tgcgatcacc gcatcgccaa tctcagcggc agtggtttac atgtcttccg
     7861 tgatggaagg tcatggcatc agctacctcc atctgctctc cgtggtcatc ccgtccaccc
     7921 tgctggcggt tctggtgatg tccttcctgg tcactatgct gttcaactcc aaactctctg
     7981 acgatccgat ttatcgcaag cgtctggaag agggcctggt tgaactgcgc ggtgaaaagc
     8041 agattgaaat caaatccggt gcaaaaacgt ccgtctggct gttcctgctg ggcgtagttg
     8101 gcgtggttat ctatgcaatc atcaacagcc caagcatggg tctggttgaa aaaccactga
     8161 tgaacaccac caacgcaatc ctgatcatca tgctcagtgt tgcaactctg accaccgtta
     8221 tctgtaaagt cgataccgac aacattctca actccagcac cttcaaagca ggtatgagcg
     8281 cctgtatttg tatcctgggt gttgcgtggc tgggcgatac tttcgtttcc aacaacatcg
     8341 actggatcaa agataccgct ggtgaagtga ttcagggtca tccgtggctg ctggccgtca
     8401 tcttcttctt tgcttctgct ctgctgtact ctcaggctgc aaccgcaaaa gcactgatgc
     8461 cgatggctct ggcactgaac gtttctccgc tgaccgctgt tgcttctttt gctgcggtgt
     8521 ctggtctgtt cattctgccg acctacccga cgctggttgc tgcggtacag atggatgaca
     8581 cgggtactac ccgtatcggt aaattcgtct tcaaccatcc gttcttcatc ccgggtactc
     8641 tgggtgttgc cctggccgtt tgcttcggct tcgtgctggg tagcttcatg ctgtaatgac
     8701 ccatcgcggg gcgttcacgc cccgctttct ttcccgccga ctaacatcct ttccccgtcc
     8761 gttgtatagt gacctctctc ttgcagttcc atctgttctt gcgaggtgtt tatgcttgat
     8821 gaaaaaagtt cgaataccac gtctgtcgtg gtgctatgta cggcaccgga tgaagcgaca
     8881 gcccaggatt tagccgccaa agtgctggcg gaaaaactgg cggcctgcgc gaccttgatc
     8941 cccggcgcta cctctctcta ttactgggaa ggtaagctgg agcaagaata cgaagtgcag
     9001 atgattttaa aaactaccgt atctcaccag caggcactgc tggaatgcct gaagtctcat
     9061 catccatatc aaaccccgga acttctggtt ttacctgtta cacacggaga cacagattac
     9121 ctctcatggc tcaacgcatc tttacgctga tcctgctact ttgcagcact tccgtttttg
     9181 ccggattatt cgacgcgccg ggacgttcac aatttgtccc cgcggatcaa gcctttgctt
     9241 ttgattttca gcaaaaccaa catgacctta atctgacctg gcagatcaaa gacggttact
     9301 acctctaccg taaacagatc cgcattacgc cggaacacgc gaaaattgct gacgtgcagc
     9361 tgccgcaagg cgtctggcat gaagatgagt tttacggcaa aagcgagatt taccgcgatc
     9421 ggctgacgct tcccgtaacc atcaaccagg caagtgcggg agcaacgtta actgtcacct
     9481 accagggctg tgctgatgcc ggtttctgtt atccgccaga aaccaaaacc gttccgttaa
     9541 gcgaagtggt cgccaacaac gaagcgtcac agcctgtgtc tgttccgcag caagagcagc
     9601 ccaccgcgca attgcccttt tccgcgctct gggcgttgtt gatcggtatt ggtatcgcct
     9661 ttacgccatg cgtgctgcca atgtacccac tgatttctgg catcgtgctg ggcggtaaac
     9721 agcggctttc cactgccaga gcattgttgc tgacctttat ttatgtgcag gggatggcgc
     9781 tgacttacac ggcgctgggt ctggtggttg ccgccgcagg gttacagttc caggcggcgc
     9841 tacagcaccc atacgtgctc attggcctcg ccatcgtctt taccttgctg gcgatgtcaa
     9901 tgtttggctt gtttactctg caactcccct cttcgctgca aacacgtctc acgctgatga
     9961 gcaatcgcca acagggcggc tcacctggcg gtgtgtttat tatgggggcg attgccggac
    10021 tgatctgttc accatgcacc actgcaccgc ttagcgcgat tctgctgtat atcgcccaaa
    10081 gcgggaacat gtggctgggc ggcggcacgc tttatcttta tgcgctgggc atgggcctgc
    10141 cgctgatgct aattaccgtc tttggtaacc gcttgctgcc gaaaagcggc ccgtggatgg
    10201 aacaagtcaa aaccgcgttt ggttttgtga tcctcgcact gccggtcttc ctgctggagc
    10261 gagtgattgg tgatatatgg ggattacgct tgtggtcggc gcttggtgtc gcattctttg
    10321 gctgggcctt tatcaccagc ctacaggcca aacgcggctg gatgcgcgtg gtgcaaataa
    10381 tcctgctggc agcggcattg gttagcgtac gcccacttca ggattgggca tttggtgcaa
    10441 cacataccgc gcaaactcag acgcatctca actttacaca aatcaaaacg gtagatgagt
    10501 taaatcaggc gctcgttgaa gccaaaggca aaccagtgat gttagatctc tatgccgact
    10561 ggtgcgtcgc ctgtaaagag tttgagaaat acaccttcag cgactcgcag gtgcaaaaag
    10621 cgttagcaga tacggtctta cttcaggcca acgtcactgc caacgacgca caagatgtgg
    10681 cgctgttaaa gcatcttaat gtccttggcc taccgacaat tctctttttt gacggacaag
    10741 gccaggagca tccacaagca cgcgtcacgg gctttatgga tgctgaaacc ttcagcgcac
    10801 atttgcgcga tcgccaaccg tgaacgacac ttgcagtggg aaagacggag gaaaatagcg
    10861 tgcaacgtga agatgtactg ggagaagccc tgaaattatt agaattacaa gggattgcca
    10921 acaccacgct ggagatggtt gctgaacgtg tggattatcc actggacgag ctacgccgct
    10981 tctggccaga caaagaggca atcctctacg atgcgctgcg ttatcttagc caacagatag
    11041 atgtctggcg tcgtcagctg atgctggacg aaacgcaaac cgccgaacaa aagttgctgg
    11101 cacgttatca ggcgttatcg gagtgcgtta aaaacaaccg ctatccgggc tgtctgttta
    11161 tcgctgcctg tacgttttat cccgatcctg gccaccctat tcatcaactg gccgatcagc
    11221 aaaaaagcgc ggcctacgat ttcacccacg aactgttaac cacgctggaa gttgacgatc
    11281 cggcgatggt agcaaagcag atggaactgg tgctggaagg ctgtttaagc cgaatgctgg
    11341 tgaatcgtag ccaggcggat gtcgacaccg cacatcggct ggcggaagat atcctgcgct
    11401 tcgcccgctg ccgtcagggt ggtgcactga cctgacagaa acacagaaaa gaagcgattt
    11461 gccgcaatct taagcagttg aatcgctttt actgaaatta ggttgacgag atgtgcagat
    11521 tacggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
    11581 ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatataaac ggacctccac
    11641 ggaggtccat ttttcgtttc aggacgccac gatttaagcg tcctgccgcc aaatcaattc
    11701 taccgaactc aaccagattc tccccacatc accagcaatt tgcgggcata tcccaattcg
    11761 ggaaaatttg tttctgagct atagcgctga ctgacgtgaa atgtcgtgcg gccccgtgat
    11821 gctgttgaac gtcaaatgac gtcatcagga gcgtaatgca tccataaagc acaacatcgg
    11881 gcagaacgcc aactgatgag attttctgaa tgagaacaaa gagaaatgta tcagtccgtt
    11941 tgctcatgca aagactaaca atccattaaa atagtaagcg ctccggacaa tttttcatgg
    12001 attattttct gaacattttt ctttggcaaa gatgatgaat tttgatggta aggaaaatta
    12061 cttctggttc tcagtaaaat cctttcgtaa tacta
//
LOCUS       DACADF010000138        12032 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.140, whole genome shotgun sequence.
ACCESSION   DACADF010000138 DACADF010000000
VERSION     DACADF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.140"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            67..450
                     /locus_tag="ISB30_003780"
     CDS             67..450
                     /locus_tag="ISB30_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991370.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVMAMFKQK"
     gene            complement(514..957)
                     /locus_tag="ISB30_003781"
     CDS             complement(514..957)
                     /locus_tag="ISB30_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP1991371.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVH
                     SFLPEAPLWVAVNERDQPIGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTTVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGV"
     gene            1114..2043
                     /gene="metA"
                     /locus_tag="ISB30_003782"
     CDS             1114..2043
                     /gene="metA"
                     /locus_tag="ISB30_003782"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAP1991372.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2312..3913
                     /gene="aceB"
                     /locus_tag="ISB30_003783"
     CDS             2312..3913
                     /gene="aceB"
                     /locus_tag="ISB30_003783"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAP1991373.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGRLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLDKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3943..5247
                     /gene="aceA"
                     /locus_tag="ISB30_003784"
     CDS             3943..5247
                     /gene="aceA"
                     /locus_tag="ISB30_003784"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAP1991374.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            5372..7096
                     /gene="aceK"
                     /locus_tag="ISB30_003785"
     CDS             5372..7096
                     /gene="aceK"
                     /locus_tag="ISB30_003785"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAP1991375.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMAL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(7113..7937)
                     /gene="iclR"
                     /locus_tag="ISB30_003786"
     CDS             complement(7113..7937)
                     /gene="iclR"
                     /locus_tag="ISB30_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAP1991376.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            8138..11821
                     /gene="metH"
                     /locus_tag="ISB30_003787"
     CDS             8138..11821
                     /gene="metH"
                     /locus_tag="ISB30_003787"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP1991377.1"
                     /translation="MSSKVEQLCAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVISAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKVSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     HRKGMSVTEVERWLAPNLGYDAE"
BASE COUNT         2932 a         3070 c         3228 g         2802 t
ORIGIN      
        1 aaaaaccccg gtcaataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagatgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattcttc atgaagtggt cgcaggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcatgg caatgtttaa gcagaaataa tcatgtcacc attggtgggt acgaaaccta
      481 aagttcagcc caccgggatg agaaaaaaac cgcctacacc cccacatatg ccagattcag
      541 caacggatac ggtttcccca aatcgtccac ctcagagcgt cccgtaacct taaagcccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acagtggttg tcagttccgg
      661 ggccattgag agcgcatgct tcaccagcat ccggcccacg ccgcagccgc gcacatcagg
      721 atcgataaac agcgcatcca tatgctgccc acttaacaac ataaatccaa tcggctgatc
      781 ccgctcatta accgcgaccc acaacggtgc ttccggcaga aaggaatgca ccagctcttc
      841 cagctcggcc cgatactctg ctgataaaaa atcgtgagtg gcatcgacag aacgacacca
      901 aatcgcaacg agttcctccc cctcctcatg ccgtgagcgt cgaatactaa taaccatttt
      961 ctctcctttt cgtcattctt atattctaac gtagcctttt ccttgaaact ttctcacctt
     1021 caacatgcag gctcgacatt ggtaaatttt ctggttatct tcagctatct ggatgtctaa
     1081 acgtataagc gtatgtagtg aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta
     1141 cccgccgtca atttcttgcg tgaagaaaac gtctttgtga tgacaacttc tcgcgcgtct
     1201 ggtcaggaaa ttcgtccgct aaaggtgctt atccttaacc tgatgccgaa gaagatcgaa
     1261 actgaaaatc agtttctgcg cctgctttca aactcacctt tgcaggtcga tattcagctg
     1321 ttgcgcatcg attcccgtga atcgcgcaac acgcccgcag agcatctgaa taacttctac
     1381 tgtaactttg aagatattca ggaacaaaac tttgacggtc tgatagttac cggcgcgccg
     1441 ctgggcctgg tggagtttaa tgatgtcgct tactggccgc agatcaaaca ggtgctggag
     1501 tggtcgaaag atcacgtcac ctcgacgctg tttgtctgct gggcggtaca ggccgcgcta
     1561 aatatcctct acggcattcc taagcaaact cgcaccgaca aactctctgg cgtttatgag
     1621 catcatattc tccatcctca tgcgcttctg acgcgtggct ttgatgattc attcctggca
     1681 ccgcattcgc gctatgctga ctttccggca gcgttgattc gtgattacac cgatctggaa
     1741 attctggcag agacggaaga aggggatgca tatctgtttg ccagcaaaga taagcgaatt
     1801 gcctttgtga cgggccatcc cgaatatgat gcgcaaacgc tggcgcagga atatttccgc
     1861 gatgtggaag ccggactaga cccggaagta ccgtataact atttcccgca caatgatccg
     1921 caaaacaaac cgcgagcgag ctggcgtagt cacggtaatt tgctgtttac caactggctc
     1981 aactattacg tctaccagat cacgccatat gatctacggc acatgaatcc aacgctggat
     2041 taatcttctg tgatagtcga tcgttaagcg attcagcacc ttacctcagg caccttcggg
     2101 tgcctttttt atttccgaaa cacacctcag taagtgaata aattttattt atattgttat
     2161 caacaagtta acatgtattt ttaattaaaa tggaaattgt ttttgatttt gcattttaaa
     2221 tgagtagtct tagttgtgct gaacgaaaag cgcacaacga tccttcgttc acagtgggga
     2281 agttttcgga tccatgacga ggagctgcac gatgactgaa caggcaacaa caatcgatga
     2341 actggctttc acaaggccgt atggcgagca ggagaagcaa attcttactg ccgaagcggt
     2401 agaatttctg actgagctgg tgacgcattt tacgccacaa cgcaataaac ttctggcagc
     2461 gcgcattcag cagcagcagg atatcgataa cggaaggttg cctgatttca tttcggaaac
     2521 agcttccatt cgtgatgctg actggaaaat tcgcgggatt cctgcggact tagaagatcg
     2581 tcgcgtagag attaccggac ctgtagagcg caagatggtg atcaacgcgc tcaacgccaa
     2641 tgtgaaagtc tttatggccg atttcgaaga ttcactggca ccagactgga acaaagtgat
     2701 cgatgggcaa attaacctgc gcgatgcagt taacggcacc atcagctata ccaatgaagc
     2761 aggcaaaatt tatcagctca agcccaatcc agcggttttg atttgtcggg tacgcggtct
     2821 gcacttgccg gaaaaacatg tcacctggcg tggtgaggca atccccggta gcctgtttga
     2881 ttttgcgctc tatttcttcc acaactatca ggcactgttg gcaaagggca gcggtcccta
     2941 tttctatctg ccgaaaaccc agtcctggca ggaagcggcc tggtggagcg aagtcttcag
     3001 ctatgcagaa gatcgcttta atctgccgcg cggcaccatc aaggcgacgt tgctgattga
     3061 aacgctgccc gccgtgttcc agatggatga aatccttcac gcgctgcgtg accatattgt
     3121 tggtctgaac tgcggtcgtt gggattacat cttcagttat atcaaaacat tgaaaaacta
     3181 tcccgatcgc gtcctgccag acagacaggc agtgacgatg gataaaccct tcctgaatgc
     3241 ttactcacgc ctgttgatta aaacctgcca taaacgcggt gcttttgcga tgggcggcat
     3301 ggcggcgttt attccgagca aagatgaaga gcgcaataac caggtgctcg acaaagtaaa
     3361 agcggataaa tcgctggaag ccaataacgg tcacgatggc acatggatcg ctcacccggg
     3421 ccttgctgat acggcaatgg cggtattcaa cgatattctc ggctcccgca aaaaccagct
     3481 tgaggtgatg cgcgaacaag acgcgccgat tactgccgat cagctgctgg caccttgtga
     3541 tggtgaacgc accgaagaag gtatgcgtgc caacattcgc gtggctgtgc agtacatcga
     3601 agcgtggatc tctggcaatg gctgtgtgcc gatttatggc ctgatggaag atgcggcgac
     3661 ggctgaaatt tcccgtacct caatctggca gtggatccat catcaaaaaa cgttgagcaa
     3721 tggcaaaccg gtgactaaag ccttgttccg ccagatgctg ggcgaagaga tgaaagtcat
     3781 tgccagcgaa ctgggcgaag aacgtttctc ccaggggcgt tttgacgatg ccgcacgctt
     3841 gatggaacag atcaccactt ccgatgagtt aattgatttc ctgaccctgc caggctaccg
     3901 cctgttagcg taaaccacca cataactatg gagcatctgc acatgaaaac ccgtacacaa
     3961 caaattgaag aattacagaa agagtggact caaccgcgtt gggaaggcat tactcgcccg
     4021 tacagcgcgg aagatgtggt gaaattacgc ggttcagtca atccggaatg caccctggcg
     4081 caactgggcg cggcgaaaat gtggcgtctg ctgcacggcg agtcgaaaaa aggctacatc
     4141 aacagcctcg gtgcactgac tggcggtcag gcgttgcaac aggcgaaagc gggtattgaa
     4201 gcagtctatc tgtcgggatg gcaggtagcg gcggacgcta acctggcggc cagcatgtat
     4261 ccggatcagt cgctctatcc ggcaaactcg gtgccagctg tggtagagcg gatcaacaat
     4321 actttccgcc gtgcggatca gatccaatgg tccgcgggca ttgagccggg cgatccgcgc
     4381 tatgtcgatt acttcctgcc gatcgtcgcc gatgcggagg ccggttttgg cggtgtactg
     4441 aatgcctttg aactgatgaa agcgatgatt gaagccggtg cagcggcagt tcacttcgaa
     4501 gatcagctgg catcagtgaa gaaatgtggt cacatgggcg gcaaagtttt ggtgccaact
     4561 caggaagcta ttcagaaact ggtcgcggcg cgtctggcag ctgatgtgac gggcgttcca
     4621 accctgctgg ttgctcgtac cgatgccgat gcggcggatc tgattacctc cgattgcgac
     4681 ccatatgaca gcgaatttat tactggcgag cgtaccagtg aaggcttctt ccgtactcat
     4741 gcgggtattg agcaagcgat cagccgtggc ctggcgtatg cgccatatgc tgacctggta
     4801 tggtgtgaaa cctccacgcc ggatctggaa ctggcgcgtc gctttgcaca agccatccac
     4861 gcgaaatatc cgggcaaact gctggcttat aactgctcgc cgtcgttcaa ctggcagaaa
     4921 aacctcgacg acaaaactat tgccagcttc cagcagcagc tgtcggatat gggctacaag
     4981 ttccagttca tcaccctggc aggtatccac agcatgtggt tcaacatgtt tgacctggca
     5041 aacgcctatg cccagggtga gggtatgaag cactatgttg agaaagtgca gcagccggaa
     5101 tttgccgccg cgaaagatgg ctataccttc gtatctcacc agcaggaagt ggggacaggt
     5161 tatttcgata aagtgacgac cattattcag ggcggcacgt cttcagtcac cgcgctgacc
     5221 ggctccaccg aagaatcgca gttctaagca actgataaag ttgatgattg taggccggat
     5281 aaggcgttca cgccgcatcc ggcaatcaac gcctgatgcg acgatttgcg cgtcttatcc
     5341 ggcaattctc tgctcctgat gagggcgcta aatgccgcgt ggcctggaat tactgattgc
     5401 acaaaccatt ctgcaaggct tcgacgctca gtatggtcga ttcctcgaag tgacttccgg
     5461 ggcgcagcag cgtttcgaac aagccgactg gcacgctgtc cagcaagcga tgaaaaaccg
     5521 tatccatctt tacgatcatc acgtgggtct ggttgtggag caactgcgct gcattactaa
     5581 cggccaaagc acggacgcgg catttttact gcgcgttaaa gagcattaca cccgactgtt
     5641 gccggattac ccgcgcttcg agattgcgga gagctttttt aactccgtgt actgtcggtt
     5701 atttgaccac cgctcgctta ctcccgagcg gctttttatc tttagctccc agccagagcg
     5761 ccgttttcgt accattcccc gcccgctggc gaaagacttt caccccgatc acggctggga
     5821 atctctgctg atgcgcgtta tcagcgacct gccgttgcgt ctgcgctggc agaataaaag
     5881 ccgtgacatt cattacatcg ttcgccatct aacggaaacg ctggggacag acaacctcgc
     5941 ggaaagtcat ttacaggtgg cgaacgaact gttttaccgc aataaagccg cctggctggt
     6001 gggcaaactg atcacgcctt ccggcacatt gccatttttg ctgccgatcc accagacgga
     6061 cgacggcgag ttatttattg atacctgtct gacgacgacc gccgaagcga gcatcgtctt
     6121 tggctttgcg cgttcttatt ttatggttta cgcgccgctg cccgcagcgc tggtcgagtg
     6181 gctacgggaa attctgccag gtaaaaccac cgctgaattg tatatggcta tcggctgcca
     6241 gaagcacgcc aaaaccgaaa gctaccgcga atatctcgtt tatctacagg gctgtaatga
     6301 gcagttcatt gaagcgccgg gtattcgtgg aatggtgatg ttggtgttca cgctgccggg
     6361 ctttgaccgg gtatttaaag tgatcaaaga caagttcgcg ccgcagaaag agatgtctgc
     6421 cgctcacgtt cgcgcctgct accaactggt gaaagagcac gatcgcgtgg gccgaatggc
     6481 ggacacccag gagtttgaaa actttgtgct ggagaagcgg catatttccc cggcattaat
     6541 ggcattactg ctccaggaag cagcggaaaa aatcaccgat ctcggcgaac aaattgtgat
     6601 tcgccatctt tatattgagc ggcggatggt gccgctcaat atctggctgg aacaagtgga
     6661 aggtcagcag ttgcgcgatg ccattgaaga atacggtaac gctattcgcc agcttgccgc
     6721 tgctaacatt ttccctggcg acatgctgtt taaaaacttc ggtgtcaccc gtcacgggcg
     6781 tgtggtgttt tatgattacg atgaaatttg ctacatgacg gaagtgaact tccgcgacat
     6841 cccgccgccg cgctacccgg aggacgaact tgccagcgaa ccgtggtaca gcgtctcgcc
     6901 gggcgatgtt ttcccggaag agttccgtca ctggttatgt gccgacccgc gtattggtcc
     6961 gctatttgaa gagatgcacg ccgacttgtt ccgcgctgat tactggcgcg cactacaaaa
     7021 ccgtatacgt gaagggcatg tggaagatgt ttatgcgtat cggcgcaggc agcggtttag
     7081 cgttcgattc gtataaatca gaggcgaaaa agtcagcgta ttccaccgta tgccagcgtc
     7141 acttccttcg ccgctttaat caccatcgcg ccaaactcgg tcacgcggtc atcggtaata
     7201 cgtgaaatcg gtccggaaat tgaaattgcg gcaaacggtt cgcggtgctc atcgaaaata
     7261 cacgctgcaa ggcaacgcag ccccagcgca tgttcctcat cgtcaaatga ataaccccgt
     7321 ttgcgcgttt gggcgagatc ttcttttaaa tgcacaggag acaccagcgt tgcgtgggta
     7381 taggcatgta accctttgcg gtgcagtaac ttcgtcacct gttcttcgct cagttgggcc
     7441 agaaaggctt tacccgcacc ggaagcgtgc atcggcaatt taccgccgat aggcgcggac
     7501 attcgcatca ggtgagtaca ctgtacctgg tcgataataa tcgcttcgtg attgctttga
     7561 tcaagcaccg ccatattgac cgtttcgcca gactcttcca ttagattgcg caggataggg
     7621 tgaacaatcg ctaacaaatt acggctctgg agaaagctgc tgccgaccat aaaggcgtgt
     7681 gcgccgatcg cccagtgacc cagttcgccg acctgacgga caaagccctg ttgctgcatc
     7741 gtggttagca ggcggtgggt cgtggaattg ggtaacccgg cttgctgtgc cagttccgtg
     7801 agtgccacac tgccattgga ttcggcaatc cactccagta atttcaggcc acgcgttaaa
     7861 gactgaacct gtccagtcgc tggtgcggtg gcaacggcgg gttttctgcc gcgttttgcg
     7921 ggaatgggtg cgaccatgac agtctccttt tttctgtatc gtggaaatca ttttcatttt
     7981 tattatcagc gaatgcaata gttgctgaac agatccgatg agttaatgtt gaacaaatct
     8041 catgttgcgt ggtggtcgct tttaccacag atgtgtttat gccagtatgg tttgttgaat
     8101 ttttattaaa tctgggttaa gcgtgtcggg agcaagtgtg agcagcaaag tggaacaact
     8161 gtgtgcgcag ttaaatgaac gtattctggt gctggacggc ggtatgggca ccatgatcca
     8221 gagctatcga ctgaacgaag ccgattttcg tggtgaacgc tttgccgact ggccatgcga
     8281 tctcaaaggc aacaacgacc tgctggtgct cagcaaaccg gaagtgatct ccgccatcca
     8341 caacgcttac tttgaagcgg gtgcggatat catcgaaacc aacaccttca actccacgac
     8401 tattgcgatg gcggattacc agatggaatc cctgtcggcg gaaatcaact ttgcggcggc
     8461 gaaactggcg cgcgcctgtg ccgacgagtg gactgcccgc acgccggaga aaccgcgcta
     8521 cgttgccggt gttctcggcc cgaccaaccg cacggcgtct atttctccgg acgtcaacga
     8581 tccggcattc cgcaacatca cttttgacca gctggtggcg gcttatcgag aatccaccaa
     8641 agcgctggtg gaaggtggcg cggacctgat cctgattgaa accgttttcg acacccttaa
     8701 cgccaaagcg gcggtctttg cggtgaaaac ggagtttgaa gcgctgggcg ttgaactgcc
     8761 gattatgatc tccggcacca tcaccgacgc ctccgggcgc acgctctccg ggcagaccac
     8821 tgaagcgttt tacaactccc tgcgccacgc cgaagcgctg acctttggcc tgaactgtgc
     8881 gctggggcca gacgaactgc gccagtacgt acaggaactg tcgcgtattg cggaatgcta
     8941 tgtcaccgcc cacccgaacg ccgggctgcc caacgccttt ggtgaatacg atctcgacgc
     9001 cgacacgatg gcaaaacaga tacgtgaatg ggcagaagcg ggctttctca atatcgtcgg
     9061 cggctgctgc ggcaccacgc cacaacatat tgcagcgatg agtcgtgcag tagaaggatt
     9121 agcgccgcgc aaactaccgg aaattcccgt agcctgccgt ttgtccggcc tggagccgct
     9181 gaacattggc gaagatagcc tgtttgtgaa cgtgggtgaa cgcaccaacg tcaccggttc
     9241 cgctaagttc aagcgcctga tcaaagaaga gaaatacagc gaggcgctgg atgtggcgcg
     9301 tcagcaggtg gaaaacggtg cgcagattat cgatatcaac atggatgaag ggatgctcga
     9361 tgccgaagcg gcgatggtgc gttttctcaa tctgattgcc ggtgaaccgg atatcgctcg
     9421 tgtgccgata atgatcgact cctcgaaatg ggacgttatt gaaaaaggtc tgaagtgtat
     9481 ccagggcaaa ggcattgtta actctatctc gatgaaagag ggcgtcgacg cctttatcca
     9541 tcacgcgaaa ctgttgcgtc gctacggtgc ggcagtagtg gtaatggctt ttgacgaaca
     9601 gggccaggcc gatacccgcg cacggaaaat cgagatttgc cgtcgggcgt acaaaatcct
     9661 caccgaagag gttggcttcc cggctgaaga tatcatcttc gacccgaaca tcttcgcggt
     9721 cgcaactggc attgaagagc acaacaatta cgcacaggac tttatcggcg cgtgtgaaga
     9781 catcaaacgt gaactgccgc acgcactgat ttccggcggc gtttctaacg tgtcgttctc
     9841 gtttcgtggt aacgatcctg tgcgcgaagc cattcacgcg gtgttcctct actacgctat
     9901 tcgcaatggc atggatatgg ggatcgtcaa cgccgggcaa ctggcgattt acgacgacct
     9961 gcctaccgag ctacgcgacg cggtggagga tgtgatcctc aaccgccgcg acgatggcac
    10021 cgagcgttta ctcgagcttg ccgagaaata tcgcggcagc aaaaccgacg acaccgctaa
    10081 cacccagcag gcggagtggc gctcgtggga agtgaataaa cgtctggaat actcgctggt
    10141 caaaggcatt accgagttta tcgagcagga taccgaagaa gcccgccagc aggctacgcg
    10201 cccgattgaa gtgattgaag gcccgttgat ggacggcatg aacgtggtcg gcgacctgtt
    10261 tggcgagggc aaaatgttcc tgccgcaggt ggtgaaatcg gcgcgcgtaa tgaagcaggc
    10321 ggtggcgtat ctcgagccgt ttattgaagc cagcaaagag cagggtaaaa ccaacggcaa
    10381 gatggtgatc gccacagtga agggcgatgt ccacgacatc ggtaaaaata tcgttggcgt
    10441 ggtgctgcaa tgtaacaact acgaaattgt cgatctcggc gtgatggtgc ctgcggaaaa
    10501 aattctccgt accgctaaag aagtgaatgc tgatttgatt ggcctttcgg ggctgatcac
    10561 gccgtcgctg gacgagatgg ttaacgtggc gaaagagatg gagcgccagg gcttcaccat
    10621 tccgctattg attggcggcg cgaccacctc aaaagcgcac acggcggtga aaatcgagca
    10681 gaactacagc ggcccgacgg tgtatgtgca gaacgcatcg cgcaccgttg gtgtggtggc
    10741 ggcgctgctt tccgacactc agcgcgatga tttcgttgct cgtacccgca aggagtacga
    10801 aaccgtgcgt attcagcacg gacgaaagaa accacgcaca ccaccggtca cgctggaagc
    10861 ggcgcgtgat aacgacttcg cttttgactg gcaggcttac acgccgccgg tggcgcaccg
    10921 tctcggtgtg caggaagtcg aagccagcat cgaaacgctg cgtaattaca tcgactggac
    10981 gccgttcttt atgacctggt cgctggccgg gaagtatccg cgcattctgg aagatgaagt
    11041 ggtaggtgtt gaggcgcagc ggctgtttaa agacgccaac gacatgctgg ataaattaag
    11101 cgccgaaaaa atgctgaacc cgcgtggtgt ggtgggcctg ttcccggcaa accgtgtggg
    11161 cgatgacatt gaaatctacc gtgacgaaac gcgtacccat gtgattaatg tcagccacca
    11221 tctgcgccaa cagaccgaaa aaaccggctt cgctaactac tgtctcgctg acttcgttgc
    11281 gccgaaggtt tccggcaaag cggattacat cggcgcattt gccgtgactg gcgggctgga
    11341 agaggacgca ctggctgatg cctttgaagc acagcatgat gattacaaca aaatcatggt
    11401 gaaagcgctt gccgaccgtc tggcggaagc ctttgcggag tatctccatg agcgtgtgcg
    11461 taaagtctac tggggctatg cgccgaacga gaacctcagc aacgaagagc tgatccgcga
    11521 aaactaccag ggcatccgtc cggcaccggg ctaccctgcc tgcccggaac atacggaaaa
    11581 agccacaatc tgggagctgc tggaagtgga aaaacatacc ggtatgaaac tcacggaatc
    11641 tttcgccatg tggccgggtg catcggtttc gggttggtac ttcagtcatc cagacagcaa
    11701 gtattacgcg gtggcgcaaa ttcagcgcga tcaggttgaa gactatgccc accgtaaagg
    11761 aatgagcgtt accgaagttg agcgttggtt ggcaccgaat ctggggtatg atgccgagta
    11821 agtgataaca ccggggtatt tgccccggtt tttaatgtca taaataatgg attattgaat
    11881 atgataaaaa ttgatgaaat agattttttc gctttcgaag gatgttcaga gtccgaagtt
    11941 actgaactat taggaaaaaa ccaacaggtt ataatgattg gataattaat ataaaacagg
    12001 tgtggagtgg gtattttatg atggtcgtta tt
//
LOCUS       DACADF010000139        11505 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.141, whole genome shotgun sequence.
ACCESSION   DACADF010000139 DACADF010000000
VERSION     DACADF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.141"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            187..1275
                     /locus_tag="ISB30_003788"
     CDS             187..1275
                     /locus_tag="ISB30_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP1991378.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGMIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1317..2249)
                     /locus_tag="ISB30_003789"
     CDS             complement(1317..2249)
                     /locus_tag="ISB30_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1991379.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHRES"
     gene            complement(2341..2838)
                     /locus_tag="ISB30_003790"
     CDS             complement(2341..2838)
                     /locus_tag="ISB30_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAP1991380.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3096..3701
                     /locus_tag="ISB30_003791"
     CDS             3096..3701
                     /locus_tag="ISB30_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HAP1991381.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLANTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLKLARERGFEEIAQLLIAAGA"
     gene            3741..4604
                     /locus_tag="ISB30_003792"
     CDS             3741..4604
                     /locus_tag="ISB30_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAP1991382.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHDDKVQFDDQGITIGQHHHIEMSHCQRWLSPTLQMTAV
                     NFHLIAWQQWYDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLVLILFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4594..6141
                     /gene="fdrA"
                     /locus_tag="ISB30_003793"
     CDS             4594..6141
                     /gene="fdrA"
                     /locus_tag="ISB30_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HAP1991383.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLAEIEELFNTKAQSGSHEARYG
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAISMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVACFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLDQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETLFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6141..7559
                     /locus_tag="ISB30_003794"
     CDS             6141..7559
                     /locus_tag="ISB30_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAP1991384.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKITAANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
                     PPVTWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            7712..8662
                     /locus_tag="ISB30_003795"
     CDS             7712..8662
                     /locus_tag="ISB30_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HAP1991385.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQDGKEVIITTPECLPAALRGETGTHIIKT"
     gene            8672..10054
                     /locus_tag="ISB30_003796"
     CDS             8672..10054
                     /locus_tag="ISB30_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP1991386.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAGVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQTGFECEIVAFP
                     QHGLLLSKSESLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQRISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            10431..11480
                     /gene="yahK"
                     /locus_tag="ISB30_003797"
     CDS             10431..11480
                     /gene="yahK"
                     /locus_tag="ISB30_003797"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HAP1991387.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMVAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTITLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         2910 a         2846 c         3125 g         2624 t
ORIGIN      
        1 cctttttaag gtatttagcg taactgtttt tgagcgagca tcagaggtaa agataatctt
       61 cttgatagtg atgtgggatg ttatacgtat ggcatcgcta atgtttatgg ttacccctta
      121 tgtgtgctca ggaatccaca ggtaatcact catactgaac agcgataaaa gataaaaggt
      181 gtgttcatga attcatgtga ttttcgtgtt tttctgcaag agttcggtac aacggttcat
      241 ttgtcattgc ctggtagcgt atccgagaaa gaacgactgc tactcaagct gctgatgcag
      301 ggaatgtctg taacagaaat atcacagtac agaaatcgca gtgcaaagac catttcacat
      361 caaaagaaac agctctttga gaaactgggg attcagagcg atattacttt ctggcgggat
      421 attttctttc agtacaatcc ggagatcata tccgccacgg gtaataatag tcacaaatat
      481 attaatgata atcactatca ccatatcgtc acgcctgaag ccatcagtct ggcgttggaa
      541 aaccacgagt ttaaaccgtg gatccaaccg gttttctgcg cgcagactgg ggtactgacg
      601 ggctgtgaag tgcttgtccg ctgggaacat ccacaaacgg gaatgatccc accggatcag
      661 tttattcctc tggcggagtc atccggtctt attgtcataa tgactcgcca gctgatgaaa
      721 cagactgcgg atattctgat gccggtaaaa catttgctgc cggacaattt ccatattggt
      781 atcaacgtct cggcgggctg ttttttggct gcgggatttg aaaaagagtg tctgaacctg
      841 gttaagaaat taggtaacga taaaatcaag ctggttcttg agttgactga acgtaatcct
      901 attccggtaa cgccagaagc cagagcgata tttgacagcc ttcatcagca caatattact
      961 tttgcgctgg atgactttgg tacgggttat gcgacctatc gttacttgca ggcgttcccg
     1021 gtcgatttta ttaaaattga caagtcgttt gtgcaaatgg cgagtgtcga cgaaatctcc
     1081 ggtcatattg tggacaatat tgtcgaactg gcgcgtaagc ctggtttgag tatcgtggcg
     1141 gaaggggtag aaacccagga gcaggcggat ttaatgatcg gcaaaggcgt tcacttttta
     1201 cagggctatt tgtactctcc gccagtaccg ggtaataaat ttatctctga atgggtaatg
     1261 aaagcaggtg gttgatgtaa accgctattc acagcgcatc gggaggttgg cagcgattaa
     1321 ctctcccgat gcagtaaatc ctgatagatc cccgttaata tgccatttgg cgcaaattgt
     1381 tttttaatcc actgcgtcac ctgaccggtg gcagagtgct gggttgcgag taacatgcgt
     1441 gaatcctggc gcgggttatg gatttgtcgg gtaaccagca aggattgcgc catcgcctca
     1501 cggaccatgt aatccggcaa aaaaccaatt ccttcaccta aaatctggca ctgacatttg
     1561 gtgttgaaat cgggcaccag aatcgattcc tgcccatgca gcaaccagcc tactttttta
     1621 ttaatcgtat gcgcggtgtc ctccaccatg atattagggt agagacgcag ctggctttcg
     1681 gcgatcggct ccggcacaaa ggctaatgga tgatccggag cgatggcaaa agcccagcga
     1741 atcgcgccaa tctccgtgta atcaatgccg ccaccgtcca gcagcgtgtc gggtgcgccg
     1801 atagcgatat ttgcctgatt attaataatc gcatcccaga cgccgttata cacttcggtg
     1861 gtgacggtaa tctggcaggt aggaaactgc tttttcagca cctgtaataa gcgtgcggtg
     1921 tgtttgggcg tataaagcag ctgattaata caaatgcgca cacgcgcttc tatgccctgc
     1981 gaaatagtat cgatactgcg tttaatggcg tgaaaatcat tcagcaggtc ggtagctttg
     2041 cggaaaaaat aacgcccgga ctccgttaac tcaatgctgc gggtactgcg agtgaacagc
     2101 accacatcca gccctgtttc cattcgcttg atggtgtagc taatggcgga agtggttaaa
     2161 cccaactctt ctgcggcttt actgaagctg ccaaaacgtg cagcggtagt aaatgccaac
     2221 agattctctt cggtaaaaat tgagttcatt aaaccattcc cgccattatc gaatatgaat
     2281 ttctccagcc atgactgtag aggtatcggt taaagataga taatcatttt tgaataactt
     2341 ttaatacccg tcgcgtttag tcgtcgcttc gcttaatttt tgtcctccat attctgaaat
     2401 tatccccacg cagttacccg ccagaaaacc cagtaatgtc accgtccagt tcatcccgct
     2461 ggcaaaaaat agcgtcatgc cgagaaagcc tccgggaata aaagacaata aaccgaaacg
     2521 tccttgccag accactaccg ctgagaaagg cacggtagta atcacgctcg cccagaaccc
     2581 agaaatccca ctcaccgagg ccagccaacc ggcagccagc gcggcgaaaa atgcccacac
     2641 catcccggaa taattaaccg ccagacttct gataaacccc gatttaccgc cgcctgcggc
     2701 ataaaaactg caaaaggcga cgaagccaat ggttcctaat aattcccatc cctgagagag
     2761 cccgacatgt gaagacacta actgccaaag ccctgcacaa ataccgacgg tgacacccgt
     2821 tgccgtgagt ccgttcatct tgttcccctc tggttagaca tggacaaaag ctactcttac
     2881 atgtgcagac tatagagaaa taaaacctga ttgagtgaaa gggtatcggg tcaaagaaac
     2941 aaatattgca ctaccgcaca ctgcgaaaag attgttgaac atcattcaac aaaaaggcga
     3001 aatacggcga attaaattta agcgggttct tttatcagat tatcgttgct attctcaatt
     3061 catcgaaaac agccacaaaa accaggggaa tgtgaatgac tataaaaaat ctacctgccg
     3121 actatttatt ggctgcgcaa cagggtgata tcgataaagt aaaaacctgc cttgcgctgg
     3181 gtgtcgatat taatacctgc gatcgtcagg ggaaaacggc aattacgctg gcaagtttat
     3241 atcagcaata tgcttgcgtt caggcattaa ttgatgccgg agcggatatt aataagcaag
     3301 atcatacctg tttaaatcct tttttaatta gctgtctgaa cgatgattta acgctactac
     3361 gaattatttt accggctaaa cccgatctta attgcgtaac ccgttttggc ggtgtcggcc
     3421 tgacgcctgc ctgtgaaaaa ggccatttaa gtattgtaaa agagcttttg gcaaatacgg
     3481 agattaacgt taaccagact aaccatgttg gctggacacc gttgctggaa gcgattgtgc
     3541 ttaatgatgg tgggattaaa cagcaggcga ttgtccagtt attgctggaa cacggtgcca
     3601 gcccgcatct gaccgataaa tatggcaaaa cgccgctgaa actggcgcgg gaacggggct
     3661 ttgaagagat tgcgcagttg ctgatcgccg caggtgcata aaccgggagg cttgttatca
     3721 acacaccaga aaaacggtgt gtgtgggcgc taactgcgga tgctgatttt ctggcgcagc
     3781 gggggcaagg acaggttgaa caggtctttg ccagagcggt aaatatcgca ctcccggctc
     3841 gccagcagtt gctgacgctg ctttgtgaag agtacgacaa tgcgcctaac agttgtcggt
     3901 tggcactcac tcactttaat ggtctgttcc ggcatgatga taaggttcag tttgacgatc
     3961 aaggcattac gattggtcaa catcatcata tagagatgag tcattgccag cgttggctgt
     4021 ccccaacctt gcaaatgacc gctgtgaatt ttcaccttat cgcctggcaa cagtggtacg
     4081 acattattca tcagcacctg ggggaaaatg aaaccctgtt taattatcgc ggcgataatc
     4141 cgttttatca ggcgttaaat aaagaactac atattaaacg acgggcagtt attcaggccg
     4201 taaacgataa acaaaatatc gcctcagcag tcgccagtat gatggggtta gggattggcc
     4261 ttacgccatc agccgatgat tatctgaccg gactggtact tattttattt atttccggac
     4321 atccggcgga aaaatacaaa gaggaatttt atctcggcct gcaacgcggc agaaataata
     4381 ccacgttatt aagtgccatc acgctggaag ccgcattaca acaacgctgc cgggaaaata
     4441 ttcatcgttt tattcacaac attatttatg acatccctgg gaacgcaact caggcaatag
     4501 aaaaaattaa acatattggc tccagttccg gctgcgacat gctgtatggc atggccgatg
     4561 gttgtgcgct gagtcaaacc tacggaggga attatgtcag ttaaaatagt cattaaaccg
     4621 aatacctatt ttgattctgt ctcgctgatg tctatctcca cgcgtgcaaa taaactcgac
     4681 ggcgtcgagc aggcatttgt ggcgatggcg accgaaatga acaaaggcgt gttgaagaat
     4741 ttaggactgc tgacgccgga gctggagcag gcgaaaaacg gcgacctgat gattgtcatc
     4801 aatggtaaat cgggtgtgga caacgagcag ttgctggcgg agattgaaga actgttcaac
     4861 accaaagcgc aaagcggctc gcacgaggcg cgttacggca ctattgccag cgccaaaaag
     4921 catatcccgg aaagtaacct ggcagtgatt tcggtcaacg gtctgtttgc cgctcgcgaa
     4981 gcgcgtcagg cgctgcaaaa tgacctcaac gtgatgctgt tttccgataa tgtctcagtt
     5041 gaagatgaac tggcgctcaa acagttggcc catgaaaaag ggctgctgat gatggggcca
     5101 gactgcggca cggcgattat caacggcgcg gcgctctgct ttggtaacgc cgtgcgtcgc
     5161 ggcaacatcg gtattgttgg cgcatctggc accggcagcc aggaattgag cgtccgtatc
     5221 catgaatttg gtggcggcgt ttcacaactg atcggcaccg gcgggcgcga cctgagcgag
     5281 aaaatcggcg gactgatgat gctcgatgcc atcagcatgc tggaaaacga tccgcaaact
     5341 gaaatcattg tgcttatctc caaaccgcct gcgcctgcgg tggcccgcaa agtgctggaa
     5401 cgcgcacgcg cctgccgcaa gccagtggtg gcctgcttcc tcggtcgtgg cgaaacgcca
     5461 gtggatgagc aggggctaca gtttgctcgc ggcagcaaag aggcggcatt gaaagcggtg
     5521 atgctctccg gcgtgaaaca agaacatctc gatctgcata cgcttgacca gccgttgatt
     5581 gcggatgtgc gcgcgcgtct gcaaccgcag cagaaataca ttcgtggtct gttctgcggc
     5641 ggtacgctgt gtgacgaaac cctgttcgcg gtgatggaaa aacatggcga tgtctacagc
     5701 aatatccagc ccgatcctga attccgcctg caagatatca accgcagcat caaacacacc
     5761 ttcctcgact ttggcgatga tgacttcacc aacggcaagc cgcatccaat gattgacccg
     5821 accaaccgta tcagccgctt gatcgaagaa gcgcgcgatc cagaagtggc ggtgatcgtg
     5881 atggattttg tgctcggatt tggatcgcat gaagatccgg tcggttccac catcgaggcg
     5941 atcaaagaag cgaaagcgat cgccgctgcc gaaggacgcg agttgatcat tctcgcctat
     6001 gtgctgggca ccgatctcga tacgccatca ttagaacaac aaagccagat gctgcttgat
     6061 gccggagtga ttctggcgag cagcagcacc aataccggat tgctggcgcg tgaatttatc
     6121 tgcaaagggg aggaagcctg atgagccagt cactgtttag ccaaccgttg aacgttatta
     6181 acgtcggtat cgccatgttt agcgatgatc tgaaaaagca gcatgtagaa gtgacccaac
     6241 tcgactggac gccgccgggg cagggcaata tgcaggtggt gcaggcactg gacaacattg
     6301 ccgattcgcc gctggcggac aaaatcaccg ccgctaacca gcaggcgctg gagcgcatta
     6361 tccagtcgca tccagtgctg attggttttg atcaggcgat taacgtggtg ccgggcatga
     6421 cggcgaaaac cattcttcac gccgggccgc cggtcacctg ggaaaaaatg tgcggcgcga
     6481 tgaaaggcgc ggtcaccgga gcgctggtgt ttgaaggact ggcgaaagat ctcgacgagg
     6541 cggctgagct ggcggcttcc ggggagatca ccttctcgcc gtgccacgag cacgactgtg
     6601 tgggatcgat ggcgggcgtc acctcggcct cgatgtttat gcacatcgtg aaaaacaaaa
     6661 cctatggcaa catcgcttat accaacatga gcgagcagat ggcgaagatt ttgcgtatgg
     6721 gcgctaacga ccagagcgtg atcgaccgcc tcaactggat gcgcgatgtg caggggccaa
     6781 tgctgcgtga cgcgatgaaa attatcggcg agatcgatct gcgcttaatg ctggcgcagg
     6841 cgctgcatat gggcgatgaa tgccataacc gcaataacgc cgggacgacg ctgctgattc
     6901 aggcgctgac gccggggatt attcaggcgg gttattccgt tgagcaacag cgcgaagtgt
     6961 ttgagtttgt cgccagcagc gattacttct ccggcccaac gtggatggcg atgtgtaaag
     7021 cggcgatgga tgcggcgcac ggcattgaat acagcaccgt ggtgaccacc atggcgcgta
     7081 acggcgtcga gttcggcctg cgggtcagcg gcctgccggg gcaatggttt accggcccgg
     7141 cgcagcaggt catcggcccg atgtttgccg gttataagcc ggaagattcg gggctggata
     7201 tcggcgacag cgccatcacc gaaacctacg gtatcggcgg atttgcgatg gcgaccgcgc
     7261 ccgcgatcgt tgcgctggtg ggcggcacgg tggaagaggc tatcgatttc tcccgtcaga
     7321 tgcgtgaaat cacccttggc gaaaacccca acgtcaccat tccgctgctc ggttttatgg
     7381 gcgtgccgtc ggcaatcgac atcacccgcg tgggtagcag cggcattctg ccggtgatca
     7441 acaccgccat tgcccataaa gatgcgggcg tcggcatgat tggcgcgggc attgtgcatc
     7501 cacccttcgc ctgcttcgag aaagccattc ttggctggtg cgaacgttac ggcgtctgac
     7561 ctgcaaagta cgcgttgtct ctgccggatg cggcgtgaac gccttatccg gcctacgagt
     7621 ggcacgagaa ttgtaggcct gataagcgta gcgcatcagg cagtctggcg ttggtcataa
     7681 ccccatcacc ctctgtcgcg gacataacaa catgaaagaa cttgtggtcg ttgccattgg
     7741 tggcaacagc attatcaaag ataacgccag ccagtcgatt gagcatcagg cggaggcggt
     7801 gaaagccgtg gccgatacgg tgctggaaat gctggcttcc gattacgaca ttgtgctgac
     7861 ccacggcaac gggccgcagg tcgggctgga tttacgccgt gcggagattg cccacgagcg
     7921 cgaagggctg cccttaacgc cgctggcgaa ctgtgtggcg gatacgcaag gtggcattgg
     7981 ctatctgatc cagcaggcgc tgaacaaccg actggcgcgt cacggcgaga aaaaagccgt
     8041 cactgtggtg actcaggtgg aagtggataa aaacgatccg ggttttgccc atcccaccaa
     8101 gcccatcggc gcattcttta gtgaaagcca gcgtgacgaa ctacaaaagg caaaccctga
     8161 ctggcgtttt gttgaagatg ccggacgggg ctatcgccgc gtggtcgcct cgccggaacc
     8221 gaaacgtatt gtcgaagcac ctgccattaa ggcgctgatc caacaaggct ttgtggtgat
     8281 tggcgcgggc ggcggtggaa ttcctgtggt gcgcaccgaa gcgggagatt accaaagcgt
     8341 ggacgcggtt atcgacaaag atctctccac cgcgctactg gcccgcgaaa ttcacgccga
     8401 cattctggtg atcaccaccg gcgtcgaaaa agtctgtatc cactttggca agccgcagca
     8461 acaggcgctc gatcgcgtag atattgccac catgacccgc tatatgcagg aagggcattt
     8521 cccacctggc agcatgttgc caaaaatcat cgccagcctg acgttcctgg aacaggacgg
     8581 caaagaagtg attatcacca cgccggaatg cctgcctgcg gcgctacgcg gcgaaacggg
     8641 cacccatatt attaaaacct aaggacgtaa gatgaaagaa aacaatagcc gccgtgaatt
     8701 tctgagccag agcggtaaga tggtcaccgc cgccgcgctg tttggtacct cagtgccgct
     8761 cgcccatgcg ggggtagctg gcaccctaaa ctgcgaagcg aacaacacca tgaaaatcac
     8821 tgacccgcat tactatctcg ataacgtgct gctggaaacc ggttttgact acgaaaatgg
     8881 cgtggcggta cagacccgca cggcgcgcca gaccgtggag attcaggacg gtaaaattgt
     8941 tgccctgcgc gagaacaagc agcatccgga cgccacgctg ccgcactatg acgctggcgg
     9001 taagctgatg ctgcccacca cccgcgatat gcatattcat ctcgacaaaa ccttctacgg
     9061 cgggccgtgg cgctcgctca atcgtccggc aggcaccact atccaggaca tgatcaaact
     9121 cgagcagaaa atgctgccgg aactgcaacc gtacacgcag gaacgggcgg aaaaactgat
     9181 cgatttattg cagtcgaaag gcaccactat tgcccgcagc cactgcaata tcgaaccggt
     9241 ttccggcctg aaaaatctgc aaaatttgca ggcggtgctg gcgcgacgtc agacgggctt
     9301 cgagtgtgag atcgtcgcct tcccgcagca cggtttgctg ctgtcgaaat cggaatcctt
     9361 aatgcgcgaa gcgatgcagg cgggggcgca ttacgtcggc gggctggacc cgaccagtgt
     9421 tgatggcgcg atggaaaaat ccctcgacac catgttccag attgcgctgg actatgacaa
     9481 aggcgtcgat attcacctgc acgaaaccac gccggcaggc gtggcagcca tcaattatat
     9541 ggttgaaaca gtggagaaaa cgccacagct gaagggcaag ctgaccatca gccacgcctt
     9601 tgcgctggca acgctcaacg aacaacaggt agatgaactg gcgcaccgga tggcggcgca
     9661 gcgaatttcg atcgcttcga cggtgccgat tggcacgctg catatgccgc tcaaacagct
     9721 gcacgacaaa ggcgtaaaag tgatgaccgg caccgacagc gttatcgacc actggtcgcc
     9781 ttacggcctg ggcgacatgc tggaaaaagc caatctgtac gcgcagctct atattcgtcc
     9841 taacgaacag aacctctccc gctctctgtt tttagccact ggcgatgtat tgccgctcaa
     9901 cgaaaaaggc gagcgcgtgt ggcccaaagc gcaggatgat gccagttttg tgctggtgga
     9961 cgcctcctgt tccgccgagg cggtggcgcg tatttcgccg agaacggcga cgttccataa
    10021 agggcaactg gtgtggggga gtgtggcagg ttgatacgaa cttgatatgt ttgcaacggt
    10081 gaacagtaag agatttaagc cccagggagt ggggcggcct gagtatgagt gtgtcggcag
    10141 aaatggacat tatctttgaa gaatatgccc ttattgacgt tgtgtagata actatttgtc
    10201 tacgtgaaaa tatcatcagt tttgccgatt tttgtctttt acggatagca aatatcacac
    10261 ttataggcgc tcgccttatg gggaacgttg ctgctctgtt tcattcaggc tcgcggcatg
    10321 ttagggctgg atcgggtggt ttaaatattg gctatattca atggacgcgt tttgccgcga
    10381 tgacatatca ggcatcgtca aatacacata gctaatcagg agtaaacaca atgaagatca
    10441 aagctgttgg tgcatattcc gctaaacaac cgcttgaacc gatggatatc acccggcgtg
    10501 aaccgggacc gcatgatgtc aaaatcgaaa tcgcttactg tggcgtctgc cattccgatc
    10561 tccaccaggt ccgttcggag tgggcgggga cggtttaccc ctgcgtgccg ggtcatgaaa
    10621 ttgtggggcg cgtggtagcc gttggtgatc aggtagaaaa acatgcgccg ggcgatctgg
    10681 tcggtgtcgg ctgcattgtc gacagttgta aacattgcga agagtgtgaa gacgggctgg
    10741 aaaactactg tgatcacatg accggaacct ataactcgcc gacaccggat gaaccgggcc
    10801 atactctggg aggctactca caacagatcg tcgttcatga gcgatatgtt ctgcgtattc
    10861 gtcacccgca agagcagctg gcggcggtgg ctcctttatt gtgtgcagga atcaccacgt
    10921 actcgccgct acgccactgg caggccgggc cgggtaaaaa agtgggcgtg gtcggcatcg
    10981 gcggtctggg acatatgggg attaaactgg cccacgcgat gggggcgcat gtggtggcat
    11041 ttaccacttc tgagtcaaaa cgcgaagcgg caaaagccct gggggccgat gaagttgtta
    11101 actcacgcaa tgccgatgag atggtggctc atgtgaagag tttcgatttc attttgaata
    11161 cagtagctgc gccacataat ctcgacgatt ttaccacctt gctgaagcgc gatggcacca
    11221 taacgctggt tggtgcgcct gcgacaccgc ataaatcgcc ggaagttttc aacctgatca
    11281 tgaaacgccg cgcgatagct ggttctatga ttggcggcat tccagaaact caggagatgc
    11341 tcgatttttg cgccgaacat ggcatcgtgg ctgatataga gatgattcgg gccgatcaaa
    11401 ttaatgaagc ctatgagcga atgctgcgtg gtgatgtgaa atatcgtttt gttatcgata
    11461 atcgcacact aacagactga aaaaaattaa taaatatcct gtggt
//
LOCUS       DACADF010000140        11331 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.142, whole genome shotgun sequence.
ACCESSION   DACADF010000140 DACADF010000000
VERSION     DACADF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.142"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            250..1821
                     /gene="garD"
                     /locus_tag="ISB30_003798"
     CDS             250..1821
                     /gene="garD"
                     /locus_tag="ISB30_003798"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAP1991388.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1970..2305
                     /gene="prlF"
                     /locus_tag="ISB30_003799"
     CDS             1970..2305
                     /gene="prlF"
                     /locus_tag="ISB30_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HAP1991389.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2305..2769
                     /locus_tag="ISB30_003800"
     CDS             2305..2769
                     /locus_tag="ISB30_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HAP1991390.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2824..3633)
                     /gene="agaR"
                     /locus_tag="ISB30_003801"
     CDS             complement(2824..3633)
                     /gene="agaR"
                     /locus_tag="ISB30_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HAP1991391.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3882..5162
                     /gene="kbaZ"
                     /locus_tag="ISB30_003802"
     CDS             3882..5162
                     /gene="kbaZ"
                     /locus_tag="ISB30_003802"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HAP1991392.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPAGFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDGIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5185..5658
                     /gene="agaV"
                     /locus_tag="ISB30_003803"
     CDS             5185..5658
                     /gene="agaV"
                     /locus_tag="ISB30_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAP1991393.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5669..6448
                     /locus_tag="ISB30_003804"
     CDS             5669..6448
                     /locus_tag="ISB30_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HAP1991394.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6438..7316
                     /locus_tag="ISB30_003805"
     CDS             6438..7316
                     /locus_tag="ISB30_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP1991395.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7334..7768
                     /locus_tag="ISB30_003806"
     CDS             7334..7768
                     /locus_tag="ISB30_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP1991396.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7765..8898
                     /gene="nagA"
                     /locus_tag="ISB30_003807"
     CDS             7765..8898
                     /gene="nagA"
                     /locus_tag="ISB30_003807"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP1991397.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDALDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQQT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMHGGIVRTAFGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9248..10402
                     /locus_tag="ISB30_003808"
     CDS             9248..10402
                     /locus_tag="ISB30_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HAP1991398.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAITAESNDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVTIHPWQA"
     gene            10415..11275
                     /gene="kbaY"
                     /locus_tag="ISB30_003809"
     CDS             10415..11275
                     /gene="kbaY"
                     /locus_tag="ISB30_003809"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HAP1991399.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSRDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2713 a         2920 c         3003 g         2695 t
ORIGIN      
        1 attgtgcaaa tgctaattta attagtacta tttaaatatt attttgagca tatgcacata
       61 aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc gggaggtact gcaatccctg
      121 aaatgattga cattgatcac atttctgcat ttaaactcct gacattctta tttcacccaa
      181 tgaagttatt tatttttaaa tgagaccagg tcctcatttt aatagtccct ggctggagaa
      241 tattgcacaa tggccaacat cgaaatcaga caagaaacgc caactgcgtt ttatataaaa
      301 gttcacgaca cagataatgt ggcaattatt gttaatgata atggcctgaa agcaggaacg
      361 cgttttccgg atgggctgga attaattgaa catattcccc aggggcataa agtcgcattg
      421 ctggacattc cggctaatgg tgaaattatt cgttatggcg aggtgattgg ttacgccgtg
      481 cgcgcaatcc cacgcggaag ctggatcgac gaatcaatgg ttgtactacc ggaagcgccg
      541 ccgttacata cgctgccact ggcaaccaaa gtcccggaac ccttaccgcc gctggaagga
      601 tacacctttg agggctatcg caatgccgat ggcagcgtgg gcaccaaaaa cctgctcggc
      661 atcaccacca gcgtccactg tgtggcaggc gtggtggact acgtagtaaa aatcattgaa
      721 cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg tggggctgaa tcatttgtac
      781 ggttgtggcg tggcgattaa cgcacccgcg gcagttgtac ctattcgtac cattcacaat
      841 atttcgctga accctaactt tggcggcgaa gtaatggtga ttggtctggg ttgtgaaaag
      901 ttgcagcctg aacgcctact gactggaacg gatgatgtgc aagctattcc agtagaaagc
      961 gccagcattg tcagtttgca ggatgaaaag catgtcggtt ttcagtccat ggtcgaggat
     1021 attttgcagg tagccgaacg ccatctacaa aaactgaatc aacgtcagcg agaaacctgt
     1081 ccggcttcag aactggttgt cggcatgcag tgcggtggca gcgatgcgtt ttctggcgta
     1141 acggcaaacc cggcggttgg gtatgcgtct gatctattgg tgcgctgcgg cgcaacggtg
     1201 atgttttcag aagtcacaga agtgcgtgac gcgatccatc tgctgacacc acgcgcagtg
     1261 aacgaagagg tcggcaaacg gctgctggag gagatggagt ggtacgataa ctatctcaat
     1321 atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca acaagaaagg cggtctggca
     1381 aacgtggtag agaaggcact cggctccatt gctaaatcgg gtaaaagtgc aattgttgaa
     1441 gtgctgtcgc ccggccaacg cccgactaaa cgcggattaa tttacgccgc gacgccagcc
     1501 agcgattttg tctgtggcac gcaacaggtg gcttcgggta tcaccgtgca agtgtttacg
     1561 accggtcgtg gtacgccgta cggccttatg gcggtacccg tcattaaaat ggcgacccgc
     1621 accgagctgg cgaaccgctg gtttgattta atggatatta acgcgggcac tatcgctacc
     1681 ggcgaagaaa ccattgaaga agtgggctgg aagttgttcc actttattct cgacgtcgcc
     1741 agcgggaaga agaaaacttt ctcggatcaa tggggactgc ataaccagct ggcggtgttt
     1801 aacccggcac cggtgacctg atttcttttg gaacgtccct cgcaaaacat ggccttagtg
     1861 ccatgttttt attgtttaaa gtccacacat ccattaataa tgcatttgca ttaccttagt
     1921 tcaagcttat aatttgagca gaaaacaggc tgtaaaagga cagtaaataa tgcccgctaa
     1981 tgctcgctct aacgctgtac tgaccactga atcaaaggtc actatacgcg gacaaacaac
     2041 tatccccgcg ccagtgcgtg aggccttaaa actgaagcca ggcctggaca gcattcatta
     2101 cgaaattctg cctggtgggc aagtatttat gtgccgactg ggagatgaac aggaggatca
     2161 tactatgaat gcatttttgc gttttctgga tgcagatatc cagaacaatc cgcaaaaaac
     2221 tcgtccattc gacattcaac aaggaaagaa acttgtcgct ggcatggacg tcaacattga
     2281 cgatgagatt ggcgacgacg aataatggat tttccacaaa gggttaatgg ttgggcgcta
     2341 tatgctcatc cctgttttca ggaaacctac gatgctttag ttgccgaagt cgaggcatta
     2401 aagggaaaag atcctgaaaa ttatcagaga aaagctgcca caaagttatt ggcggtggtc
     2461 cataaagtga ttgaggagca tatcacggtc aatccatcat caccggcatt ccgtcatggc
     2521 aagtcgttag gctctgggaa aaataaagac tggtcacggg taaaatttgg tgctggtcgc
     2581 tatcgtctct tctttcgtta cagtgaaaaa gagaaagtca tcattctggg atggatgaac
     2641 gatgaaaaca cgctgcgcac ctacggtaaa aaaacagatg cctataccgt attcagtaaa
     2701 atgttaaaaa gaggacatcc tcctgccgac tgggaaagcc tcacccaaga gacagaagaa
     2761 aaccattgat ggtgttcaca atgcccgccg aacgcattgg tgttgaccag cgggcatcct
     2821 ttctcattcc ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc
     2881 cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg
     2941 taaactggaa cggttgaact tactggaatc ggtgaccacg atgatccgtt ccgcaacttc
     3001 gcacatccga cggtttaaac gggcttcatc ttcattatgc gtgctgacgc cacgctccag
     3061 atcgatcgca tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc
     3121 agcctgatca ccgtaaaaag attgcgactg acggcgcaaa tgccccccgg tcatcagcag
     3181 ctcaacgcct tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat
     3241 tacgtcggtg tgcttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggagtcgag
     3301 gatcacccga tgaccaggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat
     3361 cgcggtgttc agtgcgcttt tatcttccac tgatggctcg actgacggcg ttgtgctatc
     3421 gcagatcaac gcgccaccat aggcacgcac ggcgatcccc tgcttttcca gaaacgccag
     3481 atcgttgcgg atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac
     3541 actcccttgc tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac
     3601 tcgcttctca cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt
     3661 tcatttcgtt ttgcctatta acgcctttct attaagcaaa tgcaagccca ccttacccat
     3721 tggcgcaagc tactctcgtt tcagtggctt tcattatgtt tcttttgtga atcagatcag
     3781 aaaactatta tctttcgttt tatttttatc tcaccatgac gcagtatcaa ctgaaacaaa
     3841 acgaaagatt aatgtcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa
     3901 tggtgagaca gcacaaagcg ggcaaaacaa atgggattta tgccgtttgt tccgcacatc
     3961 cgctggtgct ggaagctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg
     4021 aagctacctc caatcaggtg gaccagtttg gcggttatac cggaatgacg cccgccggtt
     4081 ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgctgattc
     4141 tgggtggcga ccatctgggg ccaaaccgct ggcaaaatct gcctgccgct caggcaatgg
     4201 ccaatgccga tgatttgatt aaaagctacg tggcggcagg attcaaaaaa attcaccttg
     4261 attgcagcat gtcctgtcag gacgatccga ttcccttaac cgatggcatc gtggccgaac
     4321 gcgccgctcg tttggcgaaa gtggcggaag aaacctgccg tgaacatttt ggcgaagccg
     4381 atctggagta tgtcattggt accgaagtgc cggtgcctgg cggcgcgcat gaaaccttaa
     4441 gcgagctggc ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg
     4501 cttttgaaaa gcagggtctg aatgccatct ggccacgcat cattgccctg gtggttcaac
     4561 ccggcgtcga attcgatcac accaacgtta ttgattatca gcccgccaaa gcgaccgcct
     4621 taagccagat ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc
     4681 aaacgccgca atcgctgcgc cagctggtga ttgaccactt tgccattctg aaagttggcc
     4741 cggcgctgac ctttgctctg cgtgaagctc tgttctctct ggcagcgatt gaagaagaac
     4801 tagtgccagc aaaagcctgt tctggtctgc gtcaggtgct ggaaaacgtg atgctcgacc
     4861 gcccggaata ctggcaaagc cactaccacg gtgacggcaa cgcgcgtcgt ctggcgcgtg
     4921 gttatagcta ctcggatcgc gtgcgctatt actggccgga cagccagatt gatgacgctt
     4981 tcgctcatct ggttcgtaat ctggcggatt caccaattcc gctgccgctg atcagtcagt
     5041 atctgccgct gcagtacgtg aaagttcgct ccggcgagct gcagccaacg ccacgggaac
     5101 tcattatcaa ccatattcag gacatcctgg cgcagtacca cacagcctgt gaaggccaat
     5161 aagcaaaaca aagaggaaca cgctatgcca aatattgttt taagtcggat tgatgaacgc
     5221 ttgattcacg gtcaggtcgg cgttcagtgg gtaggatttg cgggggcaaa tctggtgctg
     5281 gtagccaacg atgaggttgc cgaagatccg gtacaacaaa acctgatgga aatggtactg
     5341 gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag tcatcgacaa catccatcgc
     5401 gccgccgatc gacagaaaat cctgctggtt tgtaaaacac cggccgattt cctgacgctg
     5461 gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca atatgcacta cgccaatggc
     5521 aagcaacaaa tcgccaaaac ggtttctgtg gatgcgggtg atatcgcagc atttaacgac
     5581 ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc cgacagagcc tgctgtggac
     5641 ctctttaaat tactttgagg gattcatcat ggaaatcagt ctgttgcagg catttgcgtt
     5701 gggcattatc gcctttatcg ctggcctgga tatgtttaac ggcttaaccc atatgcaccg
     5761 cccggtggtt ctcggcccgt tggtcgggct ggtacttggc gatctgcata ccggtatttt
     5821 aaccggcggt acgctggaac tggtgtggat ggggctggcc ccgctggcgg gcgcacagcc
     5881 gcctaacgtg attatcggta ctatcgtcgg cacggcgttt gccattacta ctggcgtgaa
     5941 acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtt gcagtacaga tggggattac
     6001 cttcctgttc tcggtgatgt ccggcgtgat gtcccgctgc gaccggatgg cggagaacgc
     6061 cgatactcgc ggcattgaac gcgtgaacta tctggcactg ctggcactcg gcactttcta
     6121 ttttctctgc gctttcctgc ctatctactt cggcgcggaa catgcaaaaa ccatcattga
     6181 tgtcctgccg caacgattaa ttgatggcct cggcgtcgcg ggcggcatca tgccagcaat
     6241 cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc tacatcccct acttcatcct
     6301 gggctttgtt gccgcagcct ggctcaagtt accggtgctg gctattgctg ccgctgcgct
     6361 ggcgatggcg ctgatcgacc tgctgcgtaa atctcctgaa ccgactcaac ctgcggcaca
     6421 gaaagaggaa tttgaagatg gcatctaatc aaaccaccct gccgaacgtc agcgaaaatg
     6481 aagaaacact gctgactggc gtcaatgaaa acgtgtatga agatcagagc attggcgcag
     6541 agctgacgaa aaaagatatc aaccgcgtcg cctggcgctc catgctgtta caagcgtctt
     6601 tcaactacga acgtatgcag gcttccggct ggttgtacgg tctgctgcct gctctgaaaa
     6661 agatccacac taataaacgg gacctggcgc gcgccatgaa ggggcatatg ggtttcttca
     6721 atacccatcc gtttctggtg acgtttgtta tcggcattat ccttgcgatg gagcgttcta
     6781 agcaggacgt taacagtatt cagagcacca aaattgccgt cggtgcgccg ctcggcggga
     6841 ttggcgatgc gatgttctgg ctaacgctac tgccgatttg tggcgggata ggcgcaagcc
     6901 tcgcgctaca aggctctatt cttggcgctg tcgtctttat tgtgctgttc aacgtggtgc
     6961 acctggggct gcgttttggt ctggcgcatt atgcttaccg catgggcgtg gcggctattc
     7021 cactcattaa agcaaatacc aaaaaagtcg gccatgcggc gtctatcgtt gggatgacgg
     7081 tgatcggcgc gctggtggca acctatgttc gtttaagcac cacgctggaa atcacggcgg
     7141 gcgacgcagt ggttaagtta caggctgatg ttatcgacaa actgatgcct gccttcttac
     7201 cgctggtcta caccctgacc atgttctggc tggtacgccg cggctggagt ccgctgcgcc
     7261 tgattgcagt gacagtggtt ctcggcatcg tcggtaaatt ctgccatttc ctttaaatac
     7321 aatgaggtat tagatgttaa gtattatttt gacagggcat ggcggatttg ccagcggcat
     7381 ggaaaaagca atgaagcaaa tcctcggcga gcaatcgcag tttatcgcca tcgattttcc
     7441 agaaacctca agcaccgcgc tgcttacatc gcagcttgaa gaggccatcg cccaactgga
     7501 ttgtgaagac ggcattgttt ttctgacgga tttgctgggc ggcacgccgt ttcgcgtagc
     7561 ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatc accggcacaa atttgcaact
     7621 attactggaa atggtgctgg aacgcgaagg gttaagcggt gaagagttcc gtgtgcaggc
     7681 gctggagtgt ggacaccgtg ggctaaccag tctggtggac gaacttggtc gctgccatga
     7741 ggaatgtccg gtcgaggaag gaatatgaca cacgttctgc gcgccagaag gctgctgact
     7801 gaagagggat ggctcgatga ccatcagttg cgtattgctg acggtgtaat cgcagcaatc
     7861 gaaccgattc cagtgggcgt gactgaacgc gatgcggaac tgctctgccc cgcttacatc
     7921 gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg atgacgcgcc ggatgcgctc
     7981 gacaagctgg caatgcacaa ggcacgcgaa ggtgtcggca gttggttacc gaccaccgta
     8041 accgcgccgc ttaataccat tcatgcggcg ctgaaacgta ttgctctacg ctgccagcgc
     8101 ggtgggcctg gtgcgcaagt gctggggagt tatctcgaag gaccgtactt cacgccgcag
     8161 aataaaggcg cacatccgcc ggagctgttt cgcgagctgg aaattgccga gctggatcaa
     8221 ttgattgccg tttctcagca gaccttacgc gtggtagcgc tggcaccaga aaaagaaggg
     8281 gcattgcagg ccatccgcca tcttaaacag caaaacgtac gggtgatgct ggggcatagc
     8341 gcggcgacct ggcaacaaac ccgtgccgcg tttgatgctg gtgccgacgg cctggtgcat
     8401 tgctataacg ggatgacagg tttacatcac cgcgaaccgg gaatggttgg cgcgggatta
     8461 acggacaagc gcgcctggct ggaactgata gccgatggtc atcatgtaca tccggcggca
     8521 atgtcgctct gttgttgctg tgcgaaagag aggatcgtac tgatcaccga cgcgatgcag
     8581 gcagccggga tgccggatgg tcactatacg ttatgtggtg aagaagtgca gatgcacggt
     8641 ggcattgtcc gtaccgcgtt cggtgggctg gcgggcagta cgctgtctgt cgatgcggca
     8701 gtgcgcaaca tggtcgagtt gacgggcgta acgcctgcgg aagccatcca tatggcgtcg
     8761 ctgcatccgg cgcgaatgct gggtgttgat ggtgttctgg gatcgcttaa accgggcaaa
     8821 cgcgccagcg tcgttgcgct ggatagcggg ctgcatgtgc aacaaatctg gattcagggt
     8881 caattagctt cgttttgatg gtttgctcct ttattaggcc ttcacttccc ccgtaaggcc
     8941 tttctttttc tttctttttg atctgtgcag cggtgtcgga tgcgacgcta atgcgcctta
     9001 tccgacctac ggttggtgac cgtaaggccg gataaagtat ttacgccgca tccagcaatc
     9061 ccttttactt cccttgtctt ttctttcaac gatcacagat ttcgttttat ttcctttttc
     9121 tccattgaac tttcagtttc ttttctatag attttaacca acgaaagaaa tcaccaagtg
     9181 aaatgaaacg aaaggcaagc gaaagcgaca acgccgacgt caagttcatc agactaagga
     9241 ttgagttatg ccagaaaatt acacccctgc tgctgccgca accggtacat ggactgaaga
     9301 agagatccgc catcagcctc gcgcatggat ccgttcactc accaacattg acgcgctacg
     9361 ttccgcgctc aataacttcc ttgaaccgtt actgcgcaaa gagaatctgc aaatcatcct
     9421 gaccggagcc ggaacatcgg catttatcgg tgacatcatc gcgccgtggc tcgccagcca
     9481 taccggtaaa aacttcagcg ccgtaccaac caccgatctg gtcaccaatc caatggacta
     9541 cctgaaccca gctcatccgc tactgttgat ctccttcggt cgctccggca acagcccgga
     9601 aagcgtcgcc gccgtggaac tggcaaatca atttgtacca gaatgctatc acctgccgat
     9661 cacctgcaac gaagcgggcg ctctttacca aaacgcgatc aacagcgaca acgcgtttgc
     9721 cctgctgatg cccgcagaaa cgcacgatcg cggctttgcg atgaccagca gcattaccac
     9781 catgatggcc agctgcctcg cggttttcgc acctgagacg atcaacagcc aaaccttccg
     9841 cgacgtggcg gatcgttgcc aggcgatcct gacctcactg ggcgatttca gcgaaggtgt
     9901 gtttggttac gcaccgtgga aacggatcgt ttatctcggc agcggtggct tacagggcgc
     9961 agcacgcgag tcggcgctga aagtgctgga actgacagcg ggtaaactgg cggccttcta
    10021 tgattccccg accggattcc gtcatggccc gaaatcgctg gtcgatgacg aaacgctggt
    10081 agtggtgttt gtctccagtc acccttacac ccgtcagtac gatcttgacc tgctggcaga
    10141 actccgccgt gacaaccagg caatgcgcgt gatcgccatc accgctgaaa gcaacgacgt
    10201 tatcgccgct ggcccgcata tcatcctgcc gccgtcccgt cactttatcg acgttgaaca
    10261 agcattttgc ttcctgatgt acgcccagac gtttgcactg atgcagtcgc tgcacatggg
    10321 caatacgccg gataccccat cagccagtgg caccgtaaac cgcgtggtgc aaggcgtaac
    10381 tattcatccg tggcaggcat aagaggatcg cattatgagc attatctcca ccaaatatct
    10441 gttacaggac gcccaggcca atggctacgc ggtgcctgcc tttaacatcc ataacgccga
    10501 gacgatccag gcgatcctcg aagtgtgcag tgaaatgcga tcgccggtga tcctcgccgg
    10561 gacaccaggt acctttaagc atatcgcgct ggaagagatc tacgccctgt gtagcgccta
    10621 ttccacgacc tataacatgc cgctggcact gcatctcgac caccacgaat cgctggatga
    10681 tattcgccgt aaagtccacg caggcgtgcg cagcgcgatg atcgacggca gccacttccc
    10741 gtttgccgag aacgtgaagc tggtgaaatc ggttgtcgac ttctgccact cccgggattg
    10801 cagcgtggaa gcagaactgg gccgcctcgg cggtgttgaa gatgacatga gcgttgacgc
    10861 cgaaagtgca ttcctgaccg atccacaaga agctaaacgc tttgtcgaac tgactggcgt
    10921 cgacagcctg gcggtagcga ttggtacggc gcacggctta tacagcaaaa cgccgaagat
    10981 tgatttccag cggctggcgg aaattcgtga agtggtggat gttcctctgg tgctgcatgg
    11041 tgccagcgat gtcccggatg aatttgtccg tcgcactatt gaactcggtg taacaaaagt
    11101 gaacgttgcc acagaattaa aaatagcctt cgctggcgcg gttaaagcct ggtttgcgga
    11161 aaatccgcag ggtaatgatc ctcgttatta tatgcgcgtc ggaatggatg cgatgaaaga
    11221 agtggtcaga aataaaatta atgtctgtgg ttcagcgaat cgaatttcag cataatcatt
    11281 aattttatac gttttttcag aatgcactgc ccgattaata tcgggtagta c
//
LOCUS       DACADF010000141        11241 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.143, whole genome shotgun sequence.
ACCESSION   DACADF010000141 DACADF010000000
VERSION     DACADF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.143"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            86..1390
                     /locus_tag="ISB30_003810"
     CDS             86..1390
                     /locus_tag="ISB30_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP1991400.1"
                     /translation="MDFEYIYPVLILFGSFAVMLAIGVPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKKLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(1461..2873)
                     /gene="ftsP"
                     /locus_tag="ISB30_003811"
     CDS             complement(1461..2873)
                     /gene="ftsP"
                     /locus_tag="ISB30_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAP1991401.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(2948..3685)
                     /gene="plsC"
                     /locus_tag="ISB30_003812"
                     /gene_synonym="parF"
     CDS             complement(2948..3685)
                     /gene="plsC"
                     /locus_tag="ISB30_003812"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAP1991402.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAENYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(3920..6178)
                     /gene="parC"
                     /locus_tag="ISB30_003813"
     CDS             complement(3920..6178)
                     /gene="parC"
                     /locus_tag="ISB30_003813"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP1991403.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYVAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAVIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQGYGDERRSPLHEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(6316..7923)
                     /locus_tag="ISB30_003814"
     CDS             complement(6316..7923)
                     /locus_tag="ISB30_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP1991404.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAM
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQAAQTADATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(8032..8514)
                     /locus_tag="ISB30_003815"
     CDS             complement(8032..8514)
                     /locus_tag="ISB30_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1991405.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPDAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLEMDVYVPLA
                     "
     gene            complement(8567..8959)
                     /gene="ygiW"
                     /locus_tag="ISB30_003816"
     CDS             complement(8567..8959)
                     /gene="ygiW"
                     /locus_tag="ISB30_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAP1991406.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            9111..9770
                     /gene="qseB"
                     /locus_tag="ISB30_003817"
     CDS             9111..9770
                     /gene="qseB"
                     /locus_tag="ISB30_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAP1991407.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9767..11116
                     /gene="qseC"
                     /locus_tag="ISB30_003818"
     CDS             9767..11116
                     /gene="qseC"
                     /locus_tag="ISB30_003818"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAP1991408.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQANID
                     VRLTLNANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVDFGNAKQGGFEAKVSW"
     gene            complement(11162..>11241)
                     /locus_tag="ISB30_003819"
     CDS             complement(11162..>11241)
                     /locus_tag="ISB30_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HAP1991409.1"
                     /translation="LYVFSLVICLGCGWWLFWGRFIFCY"
BASE COUNT         2652 a         2978 c         2826 g         2785 t
ORIGIN      
        1 gtcagtgggc ttattctggt ctggtattca gtaatgaata ttattgacaa ctatcgtaaa
       61 cggaattttc attaacgagg gaaatatgga ctttgaatat atctaccccg tcttaatttt
      121 atttggcagt tttgccgtca tgctggcaat aggtgtgcca attacttttg cgattggtct
      181 ttcttcgctg ttatctatta ttactgcctt accacccgat gccgccattt ctgtgatttc
      241 gcaaaagatg actgtggggc tggatggctt tacgctatta gccattccct tcttcgtgtt
      301 agccggaaac attatgaata ccggtggtat agccagacga ctggttaacc tggcgcaagc
      361 attagttggg cgtcttcctg gctcactggc tcattgtaat atcctcgcga atacactgtt
      421 tggtgcgatt tcaggttcag ccgttgcgtc agccgccgcg gtaggtggga ttatgtcacc
      481 actgcaagaa aaagagggat atgatccggc gttttcagca gcggttaata ttgcctctgc
      541 ccccattggc ctgatgatcc caccgagcaa tgtgttgatt gtttattccc ttgccagtgg
      601 cgggacttct gtcgctgcgt tgttcttagc tggatatctg ccgggcatcc tcaccgctgt
      661 tgccttaatg tttgtagctg cactttatgc ccgacgtcac cattatccgg tggccgagcg
      721 cattaattac cgtcagtttt tacaggtatt ccgcgaatca attcctagcc tgatgcttat
      781 ttttatcatt attggcggta ttatcgcggg ggtatttacg cctacagaag catcggcaat
      841 tgcggtaatt tatagtttag ccctggcgat gatttaccgg gaaatcactc ttaagaagct
      901 caatgatatt ctgttagatt cggtagtaac cagttcaatt gttctgttac tggtaggctg
      961 ctcgatgggg atgtcatggg ccatgacgaa tgctgatgtt cctgagttga tcaacgaact
     1021 gattacccgc gtttcggata acaaatgggt cattctgttt attatcaata tcattctgtt
     1081 gatcgttggt acctttatgg atatcacgcc ggcgatcctg atatttacac ctattttcct
     1141 gccgatcgcc cagcatctgg ggattgatcc gattcacttc ggcatcatta tggttttcaa
     1201 cttaaccatt ggcctttgca caccacctgt cggcaccatt ctgtttgtgg gatgcagcat
     1261 tggtaaggtc agtatcgaca gggcaataaa accattactg ccgatgtttc tggcattgtt
     1321 tgtagtaatg gcaattattt gttatttccc gcagcttagt ctgatgctgc caggattatt
     1381 ttcgacctga tacacaatga agactgcata ctgagtaatt ttcaatgccc caccactctg
     1441 tggggcattg aattggtcca ttacggtacc ggattgacca acagttgccc aatcgagcca
     1501 cggtccgcca tttccagcgt ctgactgttg aagtagaacg ggaagtgcgc ccaggaaggc
     1561 tgaccgaaat aaacaagcag ctccacttgt ccgtcaaccc aaacggtatc tttccagcct
     1621 ctgtcttccg ggaacggcat cgcgccattc acgttacgga tctggaacat cacgccttca
     1681 atatggaacg cttgcggctc gtccgcgcgc accgtccagc gttcccacgt tccttgctgc
     1741 gcagtaacat caatacggtt gacgtcccac aactgcccat taatccccgg gtcatcaccc
     1801 agactgatat cacgacttcg aatcggcgaa ccggccatga tttcagttgg cagcaagcgc
     1861 atcggaagac tgtctgtgac cagcggcaga aggccggttg ggcgcagcgt tagcaccagc
     1921 gtagaaacca gaatgctgga tggctcaaag aagccacgaa tacgatcaac aatgctcgcc
     1981 gcttcgccac aggtaatcga cacttcatca ccgttgctca tatccaccag aatctcacga
     2041 cgctcgcccg gtgccagcga aagttgcttc accgatacag gagcgggcag gaatccctga
     2101 tcgccagaaa tcacatgtaa cgggcgacca tcgctcatct gtagttgata gcgacgagag
     2161 ttcgacgcgt tcaacagacg caagcgcacc cagccacgcg agacttcaac gtacgggctt
     2221 tgtacaccgt taaccagtag cgtatcgcca acaaagccgc cgcttccagg ttcgttatat
     2281 tctggcgtac caaagttatc cagccgttta tcctgaataa tgacaggaaa atcatccaca
     2341 ccataatggt tggggatagg cagcgattta ctgacttcat cttccaccag ccacattccg
     2401 gcaaggccgt tatagacctg ctgagccgtg cggttgggag tattggcgtg ataccacaga
     2461 gtagctgcgt tctggcgaat gggcagtacg ggtgcccagt cagcgtttgg cgacatcatc
     2521 cgtgccggac cgcccatcag cgggccaggt acctgtagcc cggccaccgt cattgagaca
     2581 ttttctgtca ggcggttgct gtaaataagc ttaacatcgt cgcccttcca gacgcggata
     2641 gtcggcccca ggtaacgacc attgattccc caaaccgacg cgcgtgtccc tggcgtaaat
     2701 gaccagtgcg cacgttgtac agtcataaac agcggttgcc cacggcgaga ttcaagcagc
     2761 ggcggaacgg gtagcggttg ctgttgcccg gctgcgctgg ccttcagggg aacagcgcct
     2821 gcacaaagtg caatccccga tgcctgaatg aactgacgcc gactgagtga catatttgct
     2881 ccatgtaaaa ctggctaaag taataccgtg caggaaagtg ttccccgcaa aaatacagat
     2941 tgccgactta aacttttccg gcggcttcgc gttctgcgac ttctttatcg agctcggcga
     3001 ttttttgttc cattatcgaa cgacaatggg cagccaactc gcgaacctga tctttgccat
     3061 actgactgac gtcaattggc ggcagcattt cgacaatcac cagaccgttg tgtagccgat
     3121 taagattaat cttattcgaa gttgtagaga cgcacacggg tataatcggg acgcccgccg
     3181 caattgccgc gtgaaatgcc ccggtcttga acggcagcag gccgcgacca cggctgcggg
     3241 ttccttccgg gaacatccag atggaaatac ggcgtttttt gaagtgattc actacttccg
     3301 caatagtgcc gtgagcttta gtgcgattgt ttctgtcgat caataagttg ccggttaacc
     3361 aatacaactg cccgaagaag gggatccaca gcaaactctt tttacctacc gtcactgtcg
     3421 gcggctgcac aatgttcgat gccgtcacca tgtcataatt gttctggtgg ttagcaatat
     3481 agatagcatt gccgtagttt tcagcgtctg caggtttgcg gcactccact ttcaggccga
     3541 aaagcggcgc aagacgacca aacatatgcc caaaggtggc cacatgtttc ggattacgcg
     3601 ggctgaaaag gcagtaaatg gagccgaata cacagactaa gatgctgtaa atcacggtaa
     3661 taataagacg aaagatatat agcataacac cctctgaagg tctgagagac tggcattgta
     3721 cgtcacctga aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt
     3781 taatcgcaac gcatgaataa acaacggttt caagggaatt tttatcgttt ccggggattg
     3841 aatacgctgc cggatgacga cttaacgttt catccggcgt tccttgcaag cgggaggaaa
     3901 cagcgccctc cccggcatat tactcttcgc tatcaccgct gctggcacgg cgaggagagt
     3961 cgatctcaac acgatcgata cgctgcaaac cgcgcatcaa cgtaccgcgg cgtccacgtt
     4021 cgccagtgac tttctgtagc tcttccggac gcagtttaat tttgcgtttc ccaacatgaa
     4081 tggtcagcgt gctttgcggc ggcagaacgt acagttgcgc caggccatcc tcgccacgcg
     4141 cggcttctgc cgatggaatg ttgataatct tgttgccttt gcccttcgac agctgcggca
     4201 gatcgcttac cgggaacatc aacatacggc ctgcctgagt gattgccagc agcatatcgg
     4261 aagcatcttc aatcaccacc ggcggcataa catgggcatt ttccggtaag gtgatcaaag
     4321 ccttacctgc acggttacgc gccaccagat cgttaaaggt gcagacgaaa ccgtaacccg
     4381 catcggaagc catcagcagt ttctgatcgt cgctttccat cagcatatgg tcaacggtcg
     4441 ctccaggcgg caacgttaat ttgccggtga gcggctcgcc ctgaccacgc gccgacggca
     4501 gcgtaatcgg gtcgatggca tagctacgac cggtggaatc aacaaacact accggttggt
     4561 tgctcttacc tttcaccgcc gctttgaagc tatcacccgc tttataattc aggcccggcg
     4621 cgtcgatatc atggccttta gcgctgcgta cccagcccat ctgcgacagc acaatggtga
     4681 caggttcaga cggcagcatg tcgtgctcgc tcatcgcttt cgcttcttcg cgttcatgca
     4741 acggcgaacg acgttcgtca ccgtagcctt gcgcgtctgc ctgcagttct ttcttcagca
     4801 ggttattcat tttacgctcg gaagccaaaa tgccctgcaa ctggtcgcgc tctttttcca
     4861 gctcactctg ctcaccgcga atcttcatct cttccagttt ggcaagatga cgcagtttca
     4921 gttcgaggat cgcttccgcc tgggtttccg taaggccaaa ccgcgacatc agcgccggtt
     4981 tcggttcatc ttcattacga atgatctcaa tcacttcgtc gatattgaga aacgccacca
     5041 gcaaaccttc gaggatatgc aggcgcttga gaactttctc cagacgatag ttcagtcggc
     5101 ggcgcacggt atcgcggcgg aacaccagcc attcggagag gatttccagc aggtttttca
     5161 ccgccggacg accatccaga ccgatcatat tgaggttaat gcgatagctc ttttccagat
     5221 cggtggtagc aaagaggtgg ttcatcacct gatccatatc cacgcggttg gaacgcggca
     5281 caatcaccag acgggtcggg ttctcgtggt cagattcatc gcgcagatcg tcaaccatcg
     5341 gcagcttttt gttgcgcatt tgcgcagcaa tttgctccag tacgcgcgca cctgaaacct
     5401 gatgcggcaa tgcgctgata accaccgcgc catcttcctt cttccaaacc gcgcgcatac
     5461 gcactgaacc acgtccgttc tcgtagattt tacgaatctc ggcgcgcgaa gtgataattt
     5521 ccgcttcagt cggataatcc ggcccctgca cgatatccag cagctgatcg agcgtggttt
     5581 tcggctggtc gattaaggca attaccgcct gagccacttc acgcaggtta tgcggtggaa
     5641 tatcggtcgc catgccgacg gcaataccgg tggtgccgtt aagcaaaatg tttggcagac
     5701 gggcaggcag cattttcggc tcctgcaaag tgccgtcgaa gtttggcacc cagtcagccg
     5761 tcccctgccc cagctcgctc aatagcagct cggaatattt cgacaaccgg gattcggtgt
     5821 aacgcattgc cgcgaacgat ttcggatcgt ccggcgcccc ccagttcccc tgaccatcaa
     5881 ccagcggata acggtaagag aacggctgcg ccatcaggac catcgctaca taacaggcaa
     5941 tatcgccgtg cggatggtat ttacccagta cgtcaccgac ggtacgggcc gattttttaa
     6001 atttggcgct ggcattcagg cccagctcag acatcgcata cacaatgcgg cgctgaacag
     6061 gtttcagacc atcaccaata aacggcaacg cacggtccat gatcacgtac atggagtagt
     6121 ttaagtaggc gttttccgta aattcatgta gcgcaaggcg ctctgccata tcgctcatta
     6181 attctgattc ctcaacttat tcgccagcct tcaaaggggc aatattgccg gagatactac
     6241 attatctgcc gcgtcgagtc acaggagatc agcgtgaata gcgccgccgg acgtgctccg
     6301 gcggcgataa aggcatcaat gtgccttgat atacaactct ttgctgtaga cataatcctg
     6361 gggattatgc agcggaaaac cgccaacgta gggtttaagc agtttgatta acggctgata
     6421 gtagatggga atcagcggtg cctgctggtt gatgatcact tctgcctgct gatacagcgc
     6481 attacgcttt gttgcatcag ccgtttgtgc cgcctggttt agtaaggcgt catactgcgc
     6541 gtttttccag tggccgacgt tttcttcact atcgcttttg agcgtgttca ggaagctgga
     6601 ggcatcattg tacgtcgcat cccacgactg ccgagacagc ataaaatcac cggctcgtcg
     6661 ggcatcaaga taggttttcc actccattgt gcgcagcgtc acctgtgcac ccagccattt
     6721 tttccattcg gaagacaacg ctatcgcggt cttttcatgc agatcgtact tgttgtagaa
     6781 cagctcaaag cgtagcggat gagaggcgtc gtatcccgcc tgtttcagca agacttttgc
     6841 catcgcgacg cgctcactca ttggcttttg cagttcatcg aacgtcgtcg cgctaaagcc
     6901 ttttacctct ggcggcgtca gcgtggttgc gggcgttctc aaccccagta ccttttgcgc
     6961 aataagctgt cgatcaaccg taagatatag cgcccgacgc acccgcacat cgttaaatgg
     7021 cggtttctca aggttaaagt tgtaatattc gctgttcaga cgcgggataa ttcgtagctc
     7081 gccaggcagt gatttttcaa tggcgggaat ttgctgcgcc ggaacccagg tgagatcgac
     7141 ctctcccgcg cgatagcggt tatagccggt gaccgaatta tccagcgcca gatactcaac
     7201 ctgttgcaat actgtatgtt gcgcatcgcg gtactttgga tttttgcgtg cagtaatctt
     7261 ttcgttaact acccatttat caagcacaaa ggcaccgttg taaaccatgt tctctggctt
     7321 actccagcta tcgccatgtt tagcgatgac atgatgagga accgggaaca gcgtcggcca
     7381 ggcgagcatc gtcgtgaacc aaggaaccgg ctgctcaagc gtaacttcaa gagtacgatc
     7441 atccatcgct ttgacaccca gcgatgtaac atctgcttta cccgcaacaa tagccgcggc
     7501 attgttaatg tgcgcctgtg ccagatagcc agcaaaaggg cttgccgttt tcgggtcaac
     7561 cgcgcgctgc cagccgagga caaaatcctc tgccgtcaga ggctgaccgt ctgaccactg
     7621 caaaccgcta cgcagatgga aaatatagcg cttgccgccg tcaagtatct cccagcgttc
     7681 agcctgagcg ggctgcacct ggccttcacc gtccatccat accagacctt caaacagatc
     7741 cagcacaatc tgcgcggcgg tattctcctc taccttttgc gggtcgagcg tgcctgggtc
     7801 gctatgattg ttgtaacgaa agacttgttg cggggcgagc ggtgtgttgg cgggaacgtc
     7861 agcagcgtag agaggagccg cacttaccag gctgaccagc cataataaat ttcgcgtata
     7921 catggtattc cttaccggag aaattataag cccgacaaca atacgcctgt ggcattgttt
     7981 gctcaaactt atcgcgagtg atttggctca cactccggta aagattcccc atcacgctaa
     8041 cggtacataa acatccattt ccagtcgctg ctctgtcatc ccttcggcaa gattggtgta
     8101 gtgaaacaga atcggtgctt tacgcatttt ctcgccgctt gcaggcagcc agtggcgaat
     8161 gatgccccat atcgtgtgtg aaatatcgtc cagctcgcca acatggcggg ccacagcata
     8221 acgtccaccg gtaagttcac cattacttac gccataacga ttatcgggga tcggttcgct
     8281 aacgctgccg cagatatcaa agcgaaacgc atctggtgcg gtggtggcag ggtcgtccca
     8341 ggcgacgcca aaagtctggc tttggttaac aggagaaagc cccgtctctt tacgccacat
     8401 gataaatttc gccacgctgt agttgagcaa ctcagggcta cagcggtgcg gcaacatcgc
     8461 cacagggatt gatgggaaat cgataatatt tacatccagt gtcaggtttg tcattttgcc
     8521 tccctgcgaa attattcccg ggaacgcgtc ccgggagcgg taacaattac ggatttactt
     8581 tgcggatctg tttgacgtca atttcaacag agttccagtc tttatcgact tcaccctgaa
     8641 tctcaaccgt atctttcggc gtcaccgtca cgccgttcca gcgtttatgg tcgatatcaa
     8701 cattgatagt accgctggca tctttgaaca cgtagagatc gtcagagatg cgttcaacga
     8761 tattgccgcg tagggttacc caggtgtcgt cacgcaggga ttttgcgctt tctacagtcg
     8821 ttacgctgcc gttcggcccc tggaatcctc cggcctgact ttgcgttgcc gatgggccag
     8881 aaaaacctcc ctgctctgct gccataaccg gtgcactgca cagggccatt actgcaatta
     8941 ctgctgcgaa ttttttcatg tttattactc cctttaatgt ctgtttccga gcatttaaca
     9001 agatagtcct taacaacttc ttaagggaaa aaaataaaat ttaatgctgt acagagcgcg
     9061 ttacaacacg gtttactggc agcaaatacg gttatcgcag ggatgaaaaa atgcgaattt
     9121 tactgataga agatgacatg ctgattggcg acggcatcaa aacgggcctt agtaaaatgg
     9181 gttttagcgt cgactggttt acacaaggtc gtcagggaaa agaggcgcta tatagcgcac
     9241 cttatgatgc ggtgatcctg gatttaacct taccaggcat ggatggtcgc gatattttgc
     9301 gcgaatggcg agaaaaaggt cagcgtgagc ctgtattgat cctgactgcg cgcgatgcgc
     9361 tggcggaacg tgtagaaggg ctgcgtctgg gagctgacga ttatctgtgt aaaccttttg
     9421 cgttgataga agtcgccgcc aggctggaag ctctgatgcg ccgaaccaac ggccaggcca
     9481 gcaacgagct gcgccacggc aacgtcatgc tcgaccccgg caaacgtatc gccacgctgg
     9541 ctggcgaacc cttaacgctg aaaccaaaag aatttgccct gctggaatta ctgatgcgta
     9601 acgctggtcg ggtactgccg cgcaaactga ttgaagagaa actgtatacc tgggacgaag
     9661 aggtcaccag taatgccgtt gaagtgcatg tgcatcatct gcgacgcaaa ctcggtagtg
     9721 attttattcg taccgtgcat ggtattggct acacattagg tgagaaatga aatttaccca
     9781 acgtcttagt ctgcgagtca ggctgacgct aatcttttta attctggcct cggtgacctg
     9841 gctgctttcc agctttgtcg cctggaaaca aacaacggat aacgtcgatg agttgttcga
     9901 cacccaactg atgctgtttg ccaaacggtt aagtacgctc gatctcaacg aaatcaacgc
     9961 ggcggatcgc atggcacaga cgccaaataa attaaaacac ggtcatgttg atgacgatgc
    10021 gctgactttt gccatcttta cccacgacgg cagaatggtc cttaacgatg gcgataacgg
    10081 agaagatatt ccctatagct atcagcggga aggttttgct gacgggcaac tggtcggtga
    10141 agacgatccc tggcgttttg tctggatgac ctcacctgat ggcaaatatc gcatcgttgt
    10201 gggtcaggaa tgggaatacc gtgaagacat ggcgctggcg attgttgccg gacaattgat
    10261 cccgtggctg gtcgcactgc cgattatgtt aatcatcatg atggtgctac tgggtcgtga
    10321 actcgcgccg ctgaacaaac tggcgctggc actacgtatg cgtgacccag actcggaaaa
    10381 accactaaac gcgacgggcg tacccagcga agtgcgccca ctggttgagt cgctaaatca
    10441 actgttcgcc cgcacacatg cgatgatggt tcgtgaacga cgctttacct ccgacgcagc
    10501 tcacgaactt cgtagcccgt taacggcgct gaaagtgcaa accgaagttg ctcagctctc
    10561 tgacgatgat ccgcaggcgc ggaaaaaagc gctgctccaa ttacattccg ggatcgatcg
    10621 cgctactcgt ctggttgatc aactgctcac gctatcgcgg ctggactcac tggataacct
    10681 tcaggacgtc gcggagatcc cgcttgaaga tctcctgcaa tcgtcggtga tggatattta
    10741 ccacacggcg cagcaggcga acattgacgt gcgactgaca ctcaatgcca acggcatcaa
    10801 acgcaccgga caaccgctat tgctaagttt gttggtgcga aatttgctgg ataacgccgt
    10861 gcgttacagt ccacaaggta gcgtggtaga cgtcacgctg aatgccgata atttcatcgt
    10921 gagggataac ggccctggtg tgacaccaga ggcactggcg cgaattggcg aacgcttcta
    10981 tcgcccaccc ggacaaaccg ctaccggcag tggacttggg ctatcgattg tccagcgaat
    11041 cgccaaattg catggcatga atgttgattt tgggaatgcg aaacaaggtg gatttgaagc
    11101 gaaggtaagc tggtaacttc tctgtctatg agagccgtta aaacgactct catagacttt
    11161 actaatagca aaatataaac cgtccccaaa aaagccacca accacaaccc agacaaataa
    11221 cgagcgagaa tacatatagc c
//
LOCUS       DACADF010000142        11197 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.144, whole genome shotgun sequence.
ACCESSION   DACADF010000142 DACADF010000000
VERSION     DACADF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.144"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            26..253
                     /locus_tag="ISB30_003820"
     CDS             26..253
                     /locus_tag="ISB30_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scsC protein"
                     /protein_id="HAP1991410.1"
                     /translation="MTCRGSPYSTIILYIQTDPDEKSMETLSTNLQLARLVGVQGTPA
                     TIIGDEMIPGAVSWETLEAVVKEKLAVAHAQ"
     gene            243..749
                     /locus_tag="ISB30_003821"
     CDS             243..749
                     /locus_tag="ISB30_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003846856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HAP1991411.1"
                     /translation="MLSKLRRWLREGAILLVVLAGVIILLDVWRSPQMPAMFDSTPLH
                     TLDGETVTLASISEERPVLLYFWASWCGICRFTTPDVARLQSEGESVMTIALRSGNDG
                     EVSRWLSRKRVTFPVVNDSGGEISRNWEISVTPTLVVVSKGQVVTTTSGWTSYWGMKL
                     RLWRAAMF"
     gene            932..1747
                     /locus_tag="ISB30_003822"
     CDS             932..1747
                     /locus_tag="ISB30_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HAP1991412.1"
                     /translation="MIRSGDFFDFHFDRDNNSMRKGKIIVASMMVLCVLIFVAAAAWF
                     MLFQGEMTEPTEEEKQAAILHAAGLMKSETQDKKSVETLYHRYIIQRHVNLDSGELVA
                     GSSADTARQKCEKLAPERDPAQVRQRCTVADVFFVKDKNNEIQQVIIPVTGKGAKSMM
                     HAFLALGLDGRTVRNLYYYQQRETPFLGARVEDANWRKQWPGKRLLDNSGHPALKIVQ
                     DKPEHADEYTVDGISGATLTSTGVEKSINYWMGPQGYGQFLQRLASDRNNLNL"
     gene            2094..3980
                     /locus_tag="ISB30_003823"
     CDS             2094..3980
                     /locus_tag="ISB30_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HAP1991413.1"
                     /translation="MSVWASTNYAPLIEDIEQRLDKTAELYQQQHADEARRTVQMAYF
                     EVFENLEGPIRINISARKSYEMESAFGEIRRMIGEKKPLADVQARIDWLKAALREVEP
                     VLDGGHRLVAEEQHNALSRDDIAVHWQESFRTIDDLLAQAVTEYQAGNYSVASQHVQQ
                     AHYQGFKNSEMEMSLRQNRSAKDAASINQQFSSLIALTAQPDHLNDVSYQVTTLLQDI
                     EDILPGLPTTRDDQQVAAAQSADNIPAADEGNNRTDWAEVTTGINQSIQDAIARYQGG
                     DAKNAILDIQDTYFDRFEASGMENKIGSRDSAFKTTLEAYFTRLVSLMKAGQPVERLN
                     AEASALTQDLQKAVTMLGEGEETQWSLLLYSLMIIVREGLEALLIVAAIVAYMVKNNH
                     QDKLPLIRQSVIVALIASVITAAIFQMLFTNSGASRELLEGITMLIAVVMLFFMSYWL
                     LSKVEARHWKAWLEGKLSHSLSRGSLVGLWLTSFLAVYREGAETVLFYYALIGDANTV
                     SGHMAIGAGFVIGCVVLLLAWLVMRYSVVRLPLKPFFMFTGSFMYLMAFVFAGKGVLE
                     LVEGKLFQPTLINGFPEISWLGIYPYVETLLPQVVLLLAALVALWVMRRKSAVPGETI
                     KNNP"
     gene            4021..4548
                     /locus_tag="ISB30_003824"
     CDS             4021..4548
                     /locus_tag="ISB30_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HAP1991414.1"
                     /translation="MTMKKTLIASAVMASIFIAPAAFAFKEYPAGEPVTMNEMELAAV
                     YLQPIDMEPRGMGLPAAKADVHLEADIHAVEGNKNGFGAGEWIPYLTISYTLVNNDTG
                     EKQEGTFMPMVASDGPHYGANIKMMGVGNYKVTYHIEPPSKAGMHRHTDSETGVGRWW
                     KPFDVSYEFKYVGLN"
     gene            4652..6031
                     /locus_tag="ISB30_003825"
     CDS             4652..6031
                     /locus_tag="ISB30_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003836820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HAP1991415.1"
                     /translation="MSYFFVTTLQVFFCIALLSGVLWSRNDPPSLRPLTWTLLTGLIA
                     GVLAGLFIHGSQPVQLLLVGAEVMVSLLFVLSFWWASTRIRYLWQGILIFGAARHWAL
                     DPNLGGLTSTHVLNTDLLLNLTAVVLAFAILCLAGVLCAMLLRRIRGLYWPLTLILLV
                     MIWLPLSGNLLLLLMKLQVVPLGKSLLSFVAKVTNNTALYNWAGAALLLALALCWLPA
                     LLRAFRQTRETEEPIAHRLALAQRRNALRLWLVTIGCAVVVIAGQLWWDKVASQPPQL
                     SEAVPVTLGSDGMVRLPVEQLRDGKLHRFVWVADDGKAVRFFVINRYPDKLRFGVVFD
                     ACLLCGDQGYVMEGNQVICVACGVHIFIPSIGKAGGCNPVPIENWHNDEKELVIPGKE
                     LATGVNYFSTVMTIKVTDPVDGSTLTNTSADYKYSYGGKTWFFSSEANYERFRETPEQ
                     FVPADMREE"
     gene            6034..7317
                     /locus_tag="ISB30_003826"
     CDS             6034..7317
                     /locus_tag="ISB30_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1991416.1"
                     /translation="MLWRMLRQSWGRNLRRKVLAIITVFLASSLISALLAVSIDIGDK
                     MSRELKSYGANILIEPAGQAALPALFSESSNPLSGQDFLDEAELPNIKDIFWRNNIVG
                     FAPMLGGEASVEGEPVRILGTFFSQPVDIPDEEGYETGQKTVSPYWQVTGDWPQEPAG
                     AEPQTLVGHALARQMGWKPGDKLTLRTEGEAVQVTVSGILSSGGDEDNQLVMPLSTVQ
                     HLLGLPGKVQAIRVSALTVPENELSRRARENLDALNAEEYDLWYCTAYVSSIAHQLEE
                     AISGAEVRPVWQVAASEGVVIDKIQLLMAVVTVAALVASAMGIASLMTSTIMERAKEI
                     GLMKALGARQWQIMLLFYLEAASSGLAGGALGCIAGWGLAKAIGVMLFDAPLNFAWIV
                     VPCVLVIAVLIALIGTWFPARRIARLYPVEVLYGR"
     gene            7307..8437
                     /locus_tag="ISB30_003827"
     CDS             7307..8437
                     /locus_tag="ISB30_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP1991417.1"
                     /translation="MAAKRTMLWLLVWRALRLRFQRVSVVFAALMVGATIVTALFAVW
                     FDINTKMSEELRTFGANFYIGPGHGSSMPQQELQSILDQAPQGLVHGASPWLYGMART
                     ELEKVVMVGVWFESLQKLVPYWQVTGSWIGVSFDDRNAMIGVKLAERLNVQPGDSITL
                     VDHNQRKNLQIKGIVESGDATDNMLIVSLDVAQAWLHQPGKISHGLLSVSNDVGQVEN
                     YASRLQAQYPDLEIRPVRKVSASEGQVLDKIKGLMGLVSIVILALSSLCVNTTLMAIV
                     GERAREFALQKALGSSNGDIVRQILLETSIIALAAVACGWVLGYLLAQLLGLTVFNAA
                     ISLRLPVLPITLVLSLLVAILAAIVPVRRAVSVEPAKVLKGE"
     gene            8442..9137
                     /locus_tag="ISB30_003828"
     CDS             8442..9137
                     /locus_tag="ISB30_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004863043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1991418.1"
                     /translation="MSVTHIPQVAIETRHLYKRFGDVTALDDINLRIAQGEFVAIMGA
                     SGSGKTTLMNILTCLDTATEGQVFLDGTDAAALDEEGRRRFRAEKIGLVFQQFHLIPF
                     LTALENIMLAQHYHSVVDEAAARKVLEQVGLGHRVTHLPSQLSGGEQQRVCIARALVN
                     EPPVIFADEPTGNLDEENEQRVLDLLTDLHRQGRTIVMVTHNPALGQFADRILRLQHG
                     KYLGEEANQHALA"
     gene            9124..9609
                     /locus_tag="ISB30_003829"
     CDS             9124..9609
                     /locus_tag="ISB30_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP1991419.1"
                     /translation="MRWHNVLLLAAAILIIGCKEEKLAVGAPAPALAAFDLQGQESGL
                     ERWQGKAVYLNFWSAGCGGCLAEMDTLEALSKKWGDKVVVVAVNTDPETVRINDLLAK
                     HQITYPVVRDQLNITQERYQVIGTPTSVLIDSEGRVLELHQGMRKPSELEATFARLAA
                     R"
     gene            9634..10119
                     /locus_tag="ISB30_003830"
     CDS             9634..10119
                     /locus_tag="ISB30_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991420.1"
                     /translation="MAFIWNDESLAILRENAGILTTEQIAQLLHTNITAVRNMAYRLK
                     LSLRVTAYNHRRIAQVQALYASETLSLKEIAAKTGLTASTVQYIVYVKSKNKPYATTE
                     YVSFETENAVHYRVQKEFVDTERSLLDNISDNTRFRELYLTDGTFYCARNIKYEVFIS
                     E"
     gene            10241..10874
                     /locus_tag="ISB30_003831"
                     /pseudo
     CDS             10241..10874
                     /locus_tag="ISB30_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309185.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
     gene            complement(11024..>11197)
                     /locus_tag="ISB30_003832"
     CDS             complement(11024..>11197)
                     /locus_tag="ISB30_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1991421.1"
                     /translation="SIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLF
                     RVDQMIRQWERSQ"
BASE COUNT         2470 a         2786 c         3359 g         2582 t
ORIGIN      
        1 cagacagaaa ccgtaaacac cggcattgac atgccgggga agcccttata gtacaataat
       61 tctctatatc caaactgacc ccgatgagaa aagcatggag acgttgagca ccaatctgca
      121 actcgcccgg ctggttggcg ttcaggggac gccggcaacc atcattggcg acgagatgat
      181 ccccggcgcg gtatcgtggg agacccttga ggcggtggtg aaagaaaaac tggcggtggc
      241 ccatgctcag taaactgcgc cgctggctgc gtgaaggcgc gatcttactg gttgtgctgg
      301 ctggcgtgat aatcctgctg gatgtctggc gttctccgca gatgccggcc atgtttgaca
      361 gcacgccgct gcatacgctg gacggggaaa ccgtaacgct ggcgtcgatc agtgaagaac
      421 gcccggtgct gctctacttc tgggcgagct ggtgcggtat ctgccgtttc accacgccgg
      481 atgtcgcccg tttgcagtcg gaaggggaga gcgtcatgac catcgcgctg cgttccggta
      541 atgacgggga ggtttcccgc tggttatcgc gtaaaagggt gacgttcccc gtcgtgaatg
      601 atagcggcgg tgagatatca cgaaactggg aaatcagcgt cacgccaacg ctggtggtgg
      661 tgtcgaaggg gcaggtggta actacgacca gcggctggac cagctactgg ggcatgaagt
      721 tacgtctgtg gcgggcagcc atgttttgat agcgtgccgg ggaaacccgg cattttttaa
      781 cccattgtaa ggatgtgtaa aacgagcgga gagcgtgttt tctgttattt ttgctgcttt
      841 tttaccgacg ccagttatta ttttttaccg ctgcctgacg ttgcaaacct gaattgataa
      901 tggcatatct gttttaattg aatgaaagtc tgtgatacgt tcaggcgatt ttttcgattt
      961 ccattttgac agggataaca attcaatgcg caagggtaaa attattgttg catcaatgat
     1021 ggtgctttgt gtccttattt ttgttgcggc ggcggcatgg tttatgctgt tccagggaga
     1081 aatgacggag ccgacagagg aggaaaaaca ggcggcaatt ttacacgctg cgggcctgat
     1141 gaaatcagag acccaggata aaaaatcagt cgaaacgctc tatcatcgtt atattattca
     1201 acgccatgtt aatttagatt ccggcgaact ggtggctggc agcagcgcgg acaccgcgcg
     1261 gcagaagtgt gaaaaacttg cccctgaacg cgacccagcg caggttcgtc agcgctgtac
     1321 ggtggcggat gtgttctttg tgaaagataa aaataatgaa attcagcagg ttatcattcc
     1381 agttaccggt aagggtgcga aatccatgat gcatgcgttt cttgctctgg ggctggacgg
     1441 tcgtacggtg agaaatctgt attattatca gcaacgggaa acgccgtttc tgggggctag
     1501 ggtggaagac gccaactggc gcaaacagtg gccagggaaa cgattattag ataacagcgg
     1561 acatcctgca ttgaaaatag tacaggataa acctgaacat gccgatgaat ataccgttga
     1621 cggtatttcc ggtgcgacgt tgacatcaac cggcgttgag aaaagtatta actactggat
     1681 ggggccgcag ggatatggtc aattcttaca gcgtcttgcc agcgatcgaa ataaccttaa
     1741 tctttgaata ttgctgtacg caataacgcg ttttatttct gcctgccgat aatgaataat
     1801 atcactgtcg gcagggggat ttattcctct gttaccgcgc ctgccttgcc gttaattcca
     1861 tgagaaaaac agggagataa cgtccctttt atcaacgcat aatgtgtgtg ttcatcacga
     1921 tttgatgaaa taagtttaca taacttgtcg tctgctgaca tttttatgat ccaggtcatg
     1981 tataacgcga caaaaagata tgataataat tatcatttgt attctttggc ggtgtgcacg
     2041 tgcgtaactt ccgtaactct ttgttggtta ctctctttgg cttgtttata agcatgagtg
     2101 tctgggcgtc gacgaattat gcgcctttga ttgaagatat cgagcaacgt cttgataaaa
     2161 cagcggaact ttatcagcaa cagcatgccg acgaggcgcg ccgcacggta caaatggcct
     2221 attttgaggt gttcgagaac ctggaagggc cgatccgcat caacatttcc gcgcgcaaaa
     2281 gttacgagat ggagagcgca ttcggcgaga tccgtcgaat gattggcgag aaaaaaccgc
     2341 tcgctgacgt ccaggcgcgc attgactggc tgaaagccgc actgcgcgaa gtggaaccgg
     2401 tgctggatgg cggacatcgt ctggtggccg aagagcagca caacgcgctg tccagggacg
     2461 acattgccgt tcactggcag gagagtttca ggacgatcga cgatctgctg gcgcaggcgg
     2521 tcactgaata tcaggccggg aattacagcg ttgccagtca gcacgttcaa caggcgcact
     2581 atcagggctt taaaaactct gagatggaga tgtccctcag acaaaaccgt tctgcaaaag
     2641 atgccgcctc cattaaccaa caattctcat ccctgattgc gctgaccgcg caacctgacc
     2701 atctcaacga tgtctcctat caggtcacta cgctgctaca ggatatcgag gatatcttac
     2761 ccggcttgcc aaccacccgt gacgatcagc aggttgccgc agcgcaaagc gcagacaaca
     2821 ttccggcggc ggacgaggga aataaccgca ccgactgggc tgaggtgacg acgggaatta
     2881 accagagcat tcaggacgcg atagcgcgct atcagggcgg cgatgcgaag aatgcgattc
     2941 tggatatcca ggatacctac ttcgaccgct tcgaagccag cggcatggaa aacaaaatcg
     3001 gctctcgcga ttcggcattt aaaaccacgc tggaggcgta ttttacccgc ctggtcagtc
     3061 tgatgaaggc cggtcagccg gttgagcgcc tgaatgcaga ggccagcgcg ctgacgcagg
     3121 atctgcaaaa agccgtgacg atgctgggcg aaggcgaaga aacccagtgg agtctgctgc
     3181 tctatagtct gatgatcatc gtgcgcgagg ggctggaagc gctgctgatt gttgcggcaa
     3241 tcgtggccta catggtgaaa aacaaccatc aggataagct gccgcttatt cgtcagtcgg
     3301 taattgtggc gctgatcgcc agcgtcatca ccgccgctat cttccagatg ctgtttacta
     3361 actccggcgc cagccgtgaa ctgctggaag gcataacgat gctgattgcc gtcgtgatgc
     3421 tgttctttat gagttactgg ctgctgtcca aagtggaagc gcgccactgg aaagcctggc
     3481 tggaaggcaa actgtctcat tccctgtcgc gaggctccct ggtcggacta tggctgacca
     3541 gcttcctggc ggtgtaccgc gaaggggcgg aaacggtgct gttctattac gctctgattg
     3601 gcgatgcaaa cacggtatcc ggccatatgg cgattggcgc cggttttgtg attggctgcg
     3661 tggtgctgct gctcgcctgg ctggtgatgc gttactcagt ggtgcgcctg ccgctgaaac
     3721 ccttctttat gtttaccggc agtttcatgt atctgatggc cttcgtcttt gcgggcaaag
     3781 gcgttctgga actggtggaa ggcaaactgt tccagcctac gttgattaac ggcttccctg
     3841 aaatcagctg gctggggatt tatccctacg tggaaacgtt gttaccgcag gtcgtattgc
     3901 tgctcgcggc gctggtggcg ctgtgggtga tgcggcgcaa aagcgcggtt cccggggaga
     3961 cgataaaaaa taacccataa tcgttgtata gccgttgttt tagacgagag gatacgtttg
     4021 atgaccatga agaaaaccct gattgccagc gcagtgatgg ccagcatttt cattgcgcca
     4081 gccgcgtttg cttttaaaga gtacccggca ggcgagcctg tcaccatgaa tgaaatggag
     4141 ctggcggccg tttacctgca accgattgat atggaaccgc gcggcatggg cttaccggca
     4201 gcaaaagccg atgttcacct tgaagcggat atccacgctg tagaaggcaa taaaaacggc
     4261 tttggcgcag gggaatggat cccgtacctg accatcagct acaccctggt caacaacgac
     4321 acgggcgaaa aacaggaagg caccttcatg ccgatggttg ccagcgatgg cccgcactat
     4381 ggcgcgaaca tcaaaatgat gggcgtgggc aactataaag tgacctacca cattgaaccg
     4441 ccgtcaaaag ccgggatgca tcgccatact gacagcgaaa caggcgtagg ccgctggtgg
     4501 aaaccgtttg atgtgagcta cgagtttaaa tatgtcggtc tgaactaaaa ccgactaacc
     4561 aacgcgccag gaggattccc ccttctggcg cgttggtcag accgatcata tgcccgtcgc
     4621 ttaattcaac ccgccgtaca gagtcgaagt tatgagttac tttttcgtca cgacgctaca
     4681 ggtctttttc tgcattgcgc ttctttccgg tgttctctgg agtcgaaacg atcctccctc
     4741 tttacgtccg ctgacctgga cgctgctgac cgggctgatt gccggcgtgc tggcgggact
     4801 gtttattcac ggcagccaac cggtacaact gctgctggtt ggggcggagg tcatggtatc
     4861 gctgctgttt gtcctcagtt tctggtgggc ttcaacgcgt atccgctatc tgtggcaggg
     4921 gattctgatt ttcggcgctg cccgccattg ggcgctggac cctaatctgg gcgggctgac
     4981 cagtacgcat gtgctgaata ctgacctgct gttaaacctg acggccgtcg ttctcgcttt
     5041 tgcgatcctc tgtctggcgg gcgtactctg cgcgatgctg ctgcggcgta ttcgcggcct
     5101 gtactggccg ttaacgctga ttctgctggt gatgatctgg ctgccgctga gcggcaacct
     5161 gctgttgttg ctgatgaaat tacaggttgt tccgctggga aaatcactgc tgagcttcgt
     5221 ggcgaaagtc acaaataaca cggcgctgta taactgggcg ggagccgcgc tgctgctggc
     5281 cctggcgctg tgctggcttc cggcgctgtt gcgggctttt cgacagacgc gggaaaccga
     5341 agaaccgatt gctcaccgtc tggcgctggc gcagcgtcgc aacgctttgc ggctgtggtt
     5401 ggtcacaatc ggctgcgcgg tggtcgttat cgcaggccaa ctgtggtggg ataaagtggc
     5461 ctcgcagccg ccgcaactct ctgaagccgt gcccgtaacg ctggggagcg atggcatggt
     5521 gcgcctgccg gttgaacagc ttcgtgacgg taaactgcat cgtttcgtct gggtggcgga
     5581 cgatggcaaa gcggtgcgct tcttcgtgat taaccgctat cccgacaagc tgcgttttgg
     5641 cgtggtgttc gacgcctgcc tgctgtgcgg cgatcagggt tatgtcatgg agggcaatca
     5701 ggttatctgc gtcgcctgcg gagtgcatat ttttattccg tctatcggca aggccggtgg
     5761 ctgtaacccg gtgccgattg aaaactggca caatgatgaa aaagagctgg tgatccccgg
     5821 taaagaactg gcgacagggg tgaactactt ctcgacggtc atgactatta aagtgaccga
     5881 tccggtcgat ggttcaacgt tgaccaatac ctcagccgat tacaaataca gctatggcgg
     5941 caagacctgg ttcttctcgt cagaggctaa ctatgaacgc ttccgtgaaa cgccggagca
     6001 gtttgttcct gccgacatga gggaggaata atcatgctgt ggcggatgtt acgacaatcc
     6061 tgggggcgca atttacgccg caaagtgctg gcgataatca ccgtttttct ggcctccagc
     6121 ctgatctcgg cgctgctggc ggtgtccatt gatatcggcg acaaaatgtc cagagagctg
     6181 aagtcttatg gtgcgaacat tttgattgaa cccgccggac aggctgcgct gcccgcgctg
     6241 tttagtgaga gcagtaatcc gctttccggt caggattttc ttgatgaagc cgagctgccg
     6301 aacatcaaag atatcttctg gcgaaacaac attgtcgggt ttgcgccgat gctcggcggc
     6361 gaagccagcg tggagggaga accagtgcgg attctgggca ctttcttcag tcagccagtg
     6421 gatattcctg atgaagaggg atatgagacg gggcagaaaa cggtcagccc atactggcag
     6481 gtgaccggcg actggccgca ggaacccgca ggcgcagagc cgcagacctt agtcgggcac
     6541 gcgctggcgc ggcaaatggg ctggaaaccc ggtgataagc tgacattacg taccgaaggc
     6601 gaagcagtgc aggtcaccgt cagcggtatc ctctccagcg gcggcgatga agataaccaa
     6661 ctggtgatgc cgctaagcac ggtacagcac ctgctggggc tccccggaaa agtgcaggcg
     6721 attcgcgtct cggcgctgac ggtgccggaa aacgaactgt cccgccgcgc gcgggaaaac
     6781 ctggacgcgc tcaatgccga agagtacgac ctctggtatt gcacggccta tgtgtcgtcg
     6841 atcgcgcatc agctggaaga ggcgatttcc ggtgcggaag tgcgcccggt atggcaggtc
     6901 gcggcgtccg aaggggtggt gatcgataaa attcagttgc tgatggcggt cgttaccgtc
     6961 gcggcgctgg tggcttccgc aatggggatc gcctcgctga tgaccagcac cattatggaa
     7021 cgcgccaaag agatcggttt gatgaaagcg ctgggtgcgc gacagtggca aattatgctg
     7081 ctgttctacc tggaagcggc gtccagcggg ctggctggcg gtgcgctggg atgtattgcc
     7141 gggtgggggc tggcgaaagc gattggcgtc atgctcttcg acgcgccgct taattttgcc
     7201 tggattgtgg tgccgtgcgt actggtgatt gcggtactga ttgcgttgat cggcacctgg
     7261 ttcccggccc ggcggattgc ccggctgtat cctgtggagg tgctgtatgg ccgctaaacg
     7321 taccatgctc tggctgctgg tgtggcgggc gctgcgtctg cgttttcagc gggtcagcgt
     7381 ggtgtttgcc gcgctgatgg tgggggcgac catcgtgacg gcgctttttg ccgtctggtt
     7441 cgacattaac accaaaatga gcgaagaact gcgcaccttc ggcgcgaatt tttacatcgg
     7501 gccggggcac ggttccagta tgccgcagca ggagttgcag agcattctcg atcaggcgcc
     7561 gcaggggctg gtgcatggcg ccagtccgtg gttgtacggc atggcgcgta ccgagctgga
     7621 aaaagtggtg atggttggcg tgtggttcga gtcgctgcaa aaactggtgc cgtactggca
     7681 ggtcaccggt agctggattg gcgttagttt tgacgaccgt aatgcgatga tcggcgtcaa
     7741 actggcggaa cgcctcaacg tgcagccggg cgacagcatc acgctggtgg atcacaatca
     7801 gcgtaagaat ttgcagatta aaggcattgt ggagtctggc gatgcgacgg acaacatgct
     7861 gatcgtcagc ctcgacgtgg cgcaggcctg gctgcatcag ccgggcaaaa tcagccacgg
     7921 cttgctgagc gtcagtaacg atgtcggcca ggtggagaac tatgccagcc ggttgcaggc
     7981 gcagtatcct gacctggaga ttcgtccggt gcgtaaggtc tctgcttccg aagggcaggt
     8041 gctggataaa attaaaggtc tgatgggact ggtctcgatc gtgattctgg cgctctcttc
     8101 gctgtgcgtg aataccacgc tgatggcgat tgtcggcgag cgggcgcgcg agtttgcgct
     8161 gcaaaaagcg cttggttcca gcaacggcga tatcgtgcgg caaattctgc tggaaaccag
     8221 catcatcgcg ctggcggcgg tggcgtgcgg ctgggtgctg ggatatttgc tggcgcaact
     8281 gttgggatta acggtgttta atgccgccat atcactgcgt ctgccggtgt tgcccattac
     8341 tctggtgttg tctttactgg tcgccattct ggcggcgatt gttccggtgc gacgcgcggt
     8401 aagcgtcgag cctgctaaag tgctgaaagg agagtagcgc catgtccgtt acgcatatcc
     8461 ctcaggtggc gatcgaaacc cgccacttat acaaacggtt cggtgacgtt accgccctgg
     8521 atgacattaa tctgcgcatt gcgcagggtg aatttgtcgc cattatgggc gcttctggtt
     8581 ctggtaaaac cacgctgatg aatattctga cgtgccttga tacggcgacg gaagggcagg
     8641 tgtttcttga tggcaccgat gccgccgcgc tggatgaaga ggggcgccgt cgctttcgcg
     8701 ccgagaaaat cggcctggta tttcagcagt tccatttgat cccgttctta actgcgctgg
     8761 aaaacatcat gctcgcccag cattaccaca gcgtggtgga tgaagcggcg gcgaggaagg
     8821 tgctggaaca ggtagggctt gggcatcgtg tgacgcacct gccgagccag ctttccggtg
     8881 gtgagcagca gcgtgtgtgt atcgcccggg cgctggtcaa cgaaccgccg gttattttcg
     8941 cggatgaacc gacgggcaac ctggatgaag agaatgagca gcgggtgctg gatctgctca
     9001 ccgatctgca tcgtcagggg cgcacgattg tgatggtgac gcacaaccct gcgcttgggc
     9061 agtttgccga ccgtattctg cggctgcaac acggtaaata tctgggtgag gaggccaatc
     9121 aacatgcgct ggcataatgt gctgttactc gcggcggcga tcctgataat cgggtgtaaa
     9181 gaagaaaagc tggcggtcgg ggcaccggcc ccggcgctgg cggcgtttga tctgcaaggc
     9241 caggagtcag gccttgaacg ctggcaagga aaggcggtgt acctgaattt ttggtctgcg
     9301 ggatgcgggg ggtgcctagc ggaaatggac acgctggaag cgctgagcaa aaaatggggc
     9361 gataaagtgg tggttgtggc ggtcaatacc gaccctgaaa cggtcaggat caacgatctg
     9421 ctggcaaaac atcagataac ctatccggtg gtgcgcgatc agctcaacat tacccaggaa
     9481 cgttatcagg tgatcggcac gccgacctcc gtactgattg actccgaagg acgcgttctg
     9541 gagctacacc agggtatgcg taagccgtct gagttagagg ccacgtttgc gcgcctggcg
     9601 gcgcgatgaa cgacgcgtac ggagagatac ccaatggcat ttatctggaa cgacgaaagc
     9661 cttgcgattc tgcgggaaaa cgcgggcatc ctcacgacag agcagatagc gcaactgctt
     9721 cacactaata ttaccgccgt gcggaatatg gcctatcggc tgaaactgag ccttcgggtg
     9781 acggcgtata atcaccgacg tatagcgcag gttcaggcgc tttatgcctc tgagacgttg
     9841 agtctcaaag agatcgcggc gaaaacgggg ctgacggcca gtaccgtgca atatatcgtg
     9901 tacgtgaaat caaaaaataa gccctacgcc accacggaat atgtctcttt tgaaacggag
     9961 aacgccgttc attaccgcgt gcaaaaagaa tttgtcgata cggaacgttc cctgctggac
    10021 aacatctctg ataacacgcg atttcgtgaa ctgtatctga cggacggcac tttttattgc
    10081 gcccgcaata ttaagtatga ggtttttatc tcggagtaac cggaacacgc tgacctgccg
    10141 gttatcttct ggaatctccc gttttgcgaa accgttcccg gaaaaatgtc atatttctgt
    10201 cacactctat gaggattgat aacaacaaga ggttgtaggg atgaaaaaat tgatactcgc
    10261 cgccgctgtg gcaggggctg ttttattgtc gtccgccgcg caagcgcaga caacgccgga
    10321 aggttatcaa ctgcaacaag tgttgatgat gagccgacac aacttgcgcg cgccgttggc
    10381 caataatggc agcgtgctgg agcagtccac gccgaatcag tggccggagt gggatgtgcc
    10441 cggcgggcag ctcaccacga aaggcggtgt gctggaaatt tatatgggcc actacatgcg
    10501 agaatggctg gcggagctgg ggatggtcac gtcaggcgaa tgcccgacgc cggacacggt
    10561 atatacctat gccaacagtc tgcaacgcac cgttgcgacg gcacaattct ttatcaccgg
    10621 cgctttccct gggtgcgaca ttccggtaca tcatcaggaa aaaaatgggc acgatggacc
    10681 caacgtttaa tccggtgatc accgatgact ctgcggcgtt tagccagaaa gcggtgcagg
    10741 cgatggaaaa agagcgcagc cagatgcagc ttgacgacag ctatcagctg ctggcgcaga
    10801 tgacggacta caaagattca ccgtcctgca aagaaaaaca gcagtgttcg ctgaccgaag
    10861 ccaaagacgc ctttagggaa ggtgcgaata agcaggtcat gtcttcccaa gctgactcgt
    10921 tgattaaaat ttcgcggatc tgggccgatt tttgtcccgc aaacacatcg aatcagccta
    10981 tttaggctat tttttccacc atttctggtg ttatttccgg tttttactga gatctctccc
    11041 actgacgtat catttggtcc acccgaaaca ggttggccag ggtgaataac atcgccagtt
    11101 ggttatcgtt tttcagcagc cccttgtatc tggctttcac gaagccgaac tgccgcttga
    11161 tgatgcgaaa cgggtgctcc accttggcac ggatgct
//
LOCUS       DACADF010000143        11197 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.145, whole genome shotgun sequence.
ACCESSION   DACADF010000143 DACADF010000000
VERSION     DACADF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.145"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..283
                     /locus_tag="ISB30_003833"
     CDS             <1..283
                     /locus_tag="ISB30_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAP1991422.1"
                     /translation="KALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASAADIT
                     ARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(403..582)
                     /locus_tag="ISB30_003834"
     CDS             complement(403..582)
                     /locus_tag="ISB30_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAP1991423.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            656..2017
                     /gene="pabB"
                     /locus_tag="ISB30_003835"
     CDS             656..2017
                     /gene="pabB"
                     /locus_tag="ISB30_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAP1991424.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSHLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQAAILSLSPERFILCDNGEIQTRPIKGT
                     LPRLPDLLEDSKQAEKLANSTKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTTINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2021..2599
                     /locus_tag="ISB30_003836"
     CDS             2021..2599
                     /locus_tag="ISB30_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAP1991425.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEGEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2783..4147
                     /gene="sdaA"
                     /locus_tag="ISB30_003837"
     CDS             2783..4147
                     /gene="sdaA"
                     /locus_tag="ISB30_003837"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAP1991426.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGFSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4278..5876
                     /locus_tag="ISB30_003838"
     CDS             4278..5876
                     /locus_tag="ISB30_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP1991427.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPVMPLR"
     gene            complement(5880..7436)
                     /gene="yoaE"
                     /locus_tag="ISB30_003839"
     CDS             complement(5880..7436)
                     /gene="yoaE"
                     /locus_tag="ISB30_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAP1991428.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            7900..8871
                     /gene="manX"
                     /locus_tag="ISB30_003840"
     CDS             7900..8871
                     /gene="manX"
                     /locus_tag="ISB30_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP1991429.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPIEKAAPAPAAAAPKVAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8934..9734
                     /locus_tag="ISB30_003841"
     CDS             8934..9734
                     /locus_tag="ISB30_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP1991430.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9747..10598
                     /locus_tag="ISB30_003842"
     CDS             9747..10598
                     /locus_tag="ISB30_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAP1991431.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10653..11111
                     /locus_tag="ISB30_003843"
     CDS             10653..11111
                     /locus_tag="ISB30_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAP1991432.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2731 a         2837 c         2822 g         2807 t
ORIGIN      
        1 taaagccctg gatttcattg gtgccatgga tgtatcagcg ccaacaccaa gttcgatgaa
       61 tgaaagtacg gcgaagggaa tctttaaata tttaaaagaa ctgggagtac ccgcgagtgc
      121 cgctgatatt acggcgcgag ctgaccagga aggctggaac cctggtttca cggaaaaaat
      181 ggttggatgg gcaaaaaaaa tggagtctgg tgaacgctct gtgattaaaa atcctgaata
      241 cttttcaaca tatatgcagg aagaactaaa agcactggtt tgagttaaat tcacatcagc
      301 ataaatgggt caggacgctt ttaatcacat ataaaaagcg tcctttttct cacaatcaaa
      361 taatcatcgg cttctataac gaaagcgata cggattagtg tgttactcgt cttcatcctc
      421 aaaacgcgcc acgattcgct cgccggaatg gttggcacgc agctcttctg ccaccagcgc
      481 gattgcctgg ccgctgctca tcccttgtgc catcagttcc tggatccgct cgacggcttt
      541 ttgctgctgt tcatgggtga gtgaaggtaa acctgcaaac attgttaact cctgctaaat
      601 tgttggcgct aattatttca tgctacccgg cacatagcca gtagagtcag gactgatgaa
      661 gacgttatct cccgctgtga ttactttacc ctggcgtcag gacgccgctg aattttattt
      721 ctcccactta agccacctgc cgtgggcgat gcttttacac tccggctatg ccgatcatcc
      781 gtatagccgc tttgatattg tggtcgccga gccaatttgc actttaacca ctttcggtaa
      841 agaaaccgtt gttagtgaaa gcgaaaaacg cacaacgacc actgatgacc cgctacaggt
      901 gctccagcag gtgctggatc gtgcagacat tcgcccagcg cataacgaag atttgccatt
      961 tcagggcggc gcgctggggt tgtttggcta cgatctgggc cgccgttttg agtcactgcc
     1021 agaaattgcg gaacaagata tcgttctgcc ggatatggca gtgggtatct acgactgggc
     1081 gctggttgtc gaccaccagc gtcaaacagt ttctttgctg agtcataatg atgtcaatgc
     1141 ccgtcgggcc tggctggaaa gccagcaatt ctccccgcag gaagatttca cactcacttc
     1201 cgactggcaa tccaatatga cccgcgaaca gtacggcgaa aaatttcgcc aggtacagga
     1261 atatctgcac agcggtgatt gctatcaggt gaatctcgcc cagcgttttc atgcgaccta
     1321 ttctggcgat gaatggcagg cattccttca gcttaatcag gccaaccgcg cgccgtttag
     1381 cgctttttta cgtcttgaac aggctgctat tttaagcctt tcaccagagc ggtttattct
     1441 ttgtgataat ggtgaaatcc agacccgccc gattaaaggc acgctaccac gcctgcccga
     1501 tcttctggaa gatagcaaac aagcagaaaa actggcgaac tcaacgaaag atcgtgccga
     1561 aaatctgatg attgtcgatt taatgcgtaa tgatatcggt cgtgttgccg tagcaggttc
     1621 ggtaaaagta ccagagctgt tcgtggtgga acccttccct gccgtgcatc atctggtcag
     1681 caccataacg gcgcaactac cagaacagtt acacgccagc gatctgctgc gcgcggcttt
     1741 tcctggtggc tcaataaccg gggctccgaa agtacgggct atggaaatta tcgacgaact
     1801 ggaaccgcag cgacgcaatg cctggtgcgg cagcattggc tatttgagct tttgcggcaa
     1861 catggacacc agcattacta tccgcacgct gactaccatt aacggacaaa tttactgctc
     1921 tgcgggcggt ggaattgttg ccgatagcca ggaagaagcg gaatatcagg aaacttttga
     1981 taaagttaat aagatattac gccaactgga gaagtaagac gtggaatacc gtagcctgac
     2041 gcttgatgat tttttatcgc gctttcaact tttgcgcccg caaattaacc gggaaacctt
     2101 aaatcatcgt caggctgccg tgttaatccc catcgtccgc cgaccgcaac cggggttgtt
     2161 gctgactcag cgttcgattc atctgcgtaa acacgctgga caagtggcat tccctggagg
     2221 cgcagtcgat gacacggacg catcagttat cgccgctgcg ctgcgcgaag ctgaagaaga
     2281 ggtcgctata ccgccttccg ccgttgaggt tatcggcgtg ctgccgcccg tcgatagcgt
     2341 cactggctac caggtaaccc cagtggtcgg cattatcccg cccgatctgc cgtatcgcgc
     2401 cagtgaaggt gaagtctccg ccgtctttga aatgccgctc gcccaggcat tacatctggg
     2461 tcgttatcat cctttagata tctaccgccg tggcgattca catcgggtat ggctgtcctg
     2521 gtacgaacag tattttgtat ggggaatgac cgcaggcata attcgtgagc tggcgctgca
     2581 aattggtgtg aaaccctgac tatacttatc tttacatcta caaaacacta cttgagacaa
     2641 tcatcgcaat attagttaaa tcgcggtttt tgattagttt aattcatgtg aatagttaag
     2701 ccagtcgccg cgttccctct tacactatgc gctgttatta gttcgttact ggaagtccag
     2761 tcaccttgtc aggagtatta tcgtgattag tctattcgac atgtttaagg tggggattgg
     2821 tccctcatct tcccataccg tagggcctat gaaggcgggt aaacagttcg tcgatgatct
     2881 ggtcgaaaaa ggcttactgg atagcgttac tcgcgttgcc gtggacgttt atggttcact
     2941 gtcgctgacg ggtaaaggcc accacaccga tatcgccatt attatgggtc ttgcaggtaa
     3001 cgaacctgcc accgtggata tcgacagtat tcccggtttt attcgcgacg tagaagagcg
     3061 cgaacgtctg ctgctggcac agggacggca tgaagtggat ttcccgcgcg acaacgggat
     3121 gcgttttcat aacggtaacc tgccgctgca tgaaaacggt atgcaaatcc acgcctataa
     3181 cggcgatgaa gtcgtctaca gcaaaactta ttattccatc ggcggcggtt ttatcgtcga
     3241 tgaagaacac tttggtcagg atgctgccaa cgaagtgagc gtgccgtatc cgttcaaatc
     3301 tgccaccgaa ctgctcgcgt actgtaatga aaccggcttt tcgctgtctg gtctcgctat
     3361 gcaaaacgaa ctggcgctgc acagcaagaa agagatcgac gagtatttcg cgcatgtctg
     3421 gcaaaccatg caggcatgta tcgatcgcgg gatgaatacc gaaggcgtac tgccaggccc
     3481 gttgcgcgtg ccacgtcgtg cgtcggccct gcgccggatg ctggtttcca gcgataaact
     3541 gtctaacgat ccgatgaatg tcattgactg ggtaaacatg tttgcgctgg cagttaacga
     3601 agaaaacgcc gccggtggcc gtgtggtaac tgcgccaact aacggtgcct gcggtatcgt
     3661 tccggcagtg ctggcttact atgaccactt tattgaatca gttagcccag acatctatac
     3721 ccgttacttt atggcagcgg gcgcgattgg tgcactgtat aaaatgaacg cgtctatttc
     3781 cggtgcggaa gtcggttgcc agggcgaagt aggtgttgcc tgttcaatgg ctgctgcggg
     3841 ccttgccgag ctgttgggcg gtagcccgga acaggtttgt gtggcagcgg aaattggcat
     3901 ggaacacaac ctcggtctga cctgcgaccc cgttgcaggg caagttcagg tgccgtgcat
     3961 tgaacgtaat gccattgcct ctgtgaaggc gattaacgcc gcacggatgg ctctgcgccg
     4021 caccagtgca ccgcgcgtct cgctggataa ggtcatcgaa acgatgtacg aaaccggtaa
     4081 ggacatgaac gctaaatacc gcgaaacctc acgcggtggt ctggcaatca aagtccagtg
     4141 tgactaatac ttcttattcg cccatctgca acggatgggc gaatttattc ccgctttctc
     4201 gtctgctgta attttcccca ctacacttcc actgttgcgt caggcgtttg tcgccatacg
     4261 cttacagggt ggcccgcatg caaaaagcac aacggatcat taaaacctat cgccgtaatc
     4321 gaatgattgt ttgtacgatt tgcgcactcg ttacgctcgc ttcgaccctg agcgtgcgat
     4381 ttatttcaca gcgtaactta aatcaacaac gggtagtaca attcgccaat cacgctgtag
     4441 aggaattaga taaagtactg cttcccctac aggcaggtag cgaagtcttg cttccgctga
     4501 ttggtctgcc ctgctctgtc gcccatttgc cattacgtaa acaggcggca aaactccaaa
     4561 ctgtgcgatc cattggcctg gtgcaagacg gcacacttta ttgctccagc atttttggtt
     4621 atcgcaatgt gcccgtcgtg gacattctgg ctgaacttcc tgcaccgcaa ccacttttac
     4681 gactgacgac cgaccgtgcc ctgataaaag gcagtccggt tttgattcaa tggacgcctg
     4741 cagcgggcag tagcaatgct ggggtcatgg agatgattaa catcgactta ctgacggcaa
     4801 tgctccttga accacaactg ccgcaaatca gtagcgccag cctgacggtg gacagtcggc
     4861 atttgctcta tggtaatggg ctggtagatt cccttccgca acctgaaaac aatgaaaact
     4921 atcaggtttc ttcgcaacgc tttcctttta ccattaacgt taatggcccg ggggctacgg
     4981 cgctggcatg gcactatctt ccaacacaat taccgctggc ggtgctgcta agtttactgg
     5041 tgggctacat cgcctggctg gcgaccgctt accgaatgag cttttcccgc gagatcaatc
     5101 tgggcctggc gcaacatgag ttcgaattgt tctgtcagcc tttgcttaat gcgcgcagcc
     5161 agcaatgtat tggtgtagag attttgctgc gctggaacaa tccgcgtcag ggctggattt
     5221 caccggatgt gtttattcct atcgcggaag aacatcattt aattgtgcca ctgacccgct
     5281 atgtgatggc agaaaccatt cgtcagcgcc atgttttccc aatgagtagt cagtttcatg
     5341 ttggcattaa cgtcgcaccc agccattttc gccgtggtgt gctgataaaa gatctcaatc
     5401 agtactggtt tagcgctcac ccgattcagc aactgatcct cgaaatcacc gaacgcgatg
     5461 ccttactgga tgttgattac cggattgccc gcgagctgca tcgtaaaaac gtcaaactgg
     5521 cgattgatga cttcggcacc ggcaacagtt cattttcctg gcttgaaaca ttacgtcctg
     5581 acgtgctgaa aattgataag tcatttaccg cagctatagg ttctgacgcg gttaactcga
     5641 cggtgaccga tatcatcatc gcactggggc aaagactgaa tattgaactg gtggcggagg
     5701 gcgtagaaac gcaagaacag gcgaagtatt tgcgcagtca tggcgtgcat attttgcaag
     5761 ggtatttgta cgcacagccg atgccgctac gtgattttcc caaatggctg gcgggcagcc
     5821 aaccgccgcc cgcccggcat aatggacata tcacgcccgt tatgccgtta cgttaacgct
     5881 tactcatctt catcgtgcgc cggttgctct ttaacaatgc gaaccagatc aacacgataa
     5941 tcattggctt caatgatggt gatatgcagt ggacctacat caatcacatc gcccacacgg
     6001 ggaatgtgac catttgccga gatcacgagg cccgcgaccg tcgcgatatc atcgtcatcg
     6061 gcaaggtgct caacatcaag cgcctgctgc aaggcatgca aatctgtacc gccttttacc
     6121 agccagccgt caccgtcggt aatgatttcc ggcgtttcgt cagcgtccgg gaattcaccc
     6181 gcaatggctt ccagcacatc cagcggcgtg accagacctt gtaccacacc aaactcgttg
     6241 gtcacgataa caaagctccc gcgagcacga cgcagcacgc ccagcaggtt gatcggatcg
     6301 agggtttccg ggacgataat cgccggagac gccgaagcta tcgccgccac atcaactccc
     6361 tcttccagcg ccaccagcag ttctttagca cgtacgatgc cgataatttc atccagttca
     6421 ccgcgacata ccgggaacaa actgtgcggt gaggagagca gttgctcgcg gatttcatcg
     6481 accccgagat tagcatcaac ccagcttatt tcaccgcgcg gcgtcatgat cccgcgcaga
     6541 gaacgcgacg ccagcgtcag tacgccgtta atcatgtaac gttcttcttc ggcaaacgca
     6601 ccttccggga tcggcatcgg catcgggtta tcggcatcgt gctgaacatt ggcctgacgt
     6661 ttcccgccca tcaaacgcag gatggcatcg gcagtacgcg ctcgcagcgg caaagtcgac
     6721 tggtgacgaa taaagttgcg acgcgcaatc tggttaaaca cttcgatgat gatcgagaag
     6781 ccaatcgcgg catacaggta acctttcgga atgtggaaac cgaaaccttc tgccaccaga
     6841 ctcagaccaa tcattaacag gaagctcaga cagagcacca ccaccgtggg gtgctggtta
     6901 acaaatcgcg tcagcggttt cgatgccagc agcataaccg ccatcgcaat cactaccgcc
     6961 gccatcatca ccggcaaatg gttaaccatc cctactgcag taattaccgc atccaacgaa
     7021 aagacggcgt caaggatgac aatctgtgtg acgaccaccc agaaactggc gtagccttta
     7081 ccgtggccgg aatcatgatc gcggttttcc agccgttcat gcagttcggt tgttgctttg
     7141 aacagcaaga atatgccccc gaacaacata atcaggtcgc gtccggagaa ggagaaatcc
     7201 atgacggtaa atagcggttt ggtcagcgtg accatccatg aaatcagcga cagcagcccc
     7261 agacgcataa tcagcgccag tgataatccc agcaaacgcg ctttatcgcg ttgttttggc
     7321 ggcagtttgt cagcaagaat ggcgatgaag accaggttat cgatacccag cacaatttcg
     7381 agaacaacaa gcgtgagtag ccccgcccaa atcgaggggt ccattaagaa ttccatgaca
     7441 agctcctgct taaggaatag ctattcgacg ccagaaataa tgcaggcgta acgacaaaat
     7501 gcaaacgaaa ggtgcggcat aaagtgccag aaagacaggc gttaaaaggc ctgatgctga
     7561 aatgacgtcg gtgacgatcc atactgcggg ctactgccct atactccatg gttgttaaac
     7621 aggagttaaa catatcagag acgcctctga tttggcaaag atttaccttc ctttgcaaac
     7681 gaatgtgaca aggatatttt acctttcgaa atttctgcta atcgaaagtt aaattacgga
     7741 tcttcatcac ataaaataat tttttcgata tctaaaataa atcgcgaaac gcagggggtt
     7801 tttggttgta gcccttatct gaatcgattc gattgtggac gacgattcaa aaatacatct
     7861 ggcacgttga ggtgttaacg ataataaagg aggtagcaag tgaccattgc tattgttata
     7921 ggcacacatg gttgggctgc agagcagttg cttaaaacgg cagaaatgct gttaggcgag
     7981 caggaaaacg tcggctggat cgatttcgtt ccaggtgaaa atgccgaaac gctgattgaa
     8041 aagtacaacg ctcagttggc aaaactcgac accactaaag gcgtgctgtt tctcgttgat
     8101 acatggggag gcagcccatt caatgctgcc agccgcattg tcgtcgacaa agagcattat
     8161 gaagtcattg caggcgttaa cattccaatg ctcgtggaaa cgttaatggc ccgagatgat
     8221 gaccctagct ttgatgaact ggtggcactg gcagtagaaa caggccgtga aggcgtgaaa
     8281 gcactgaaag ccaaaccgat tgaaaaagcc gcgccagcac ccgctgccgc agcacccaaa
     8341 gtggctccaa ctccggcaaa accaatggga ccgaacgact acatggttat tggccttgcg
     8401 cgtatcgacg accgtctgat tcacggtcag gtcgccaccc gctggaccaa agaaaccaat
     8461 gtctcccgta ttattgttgt tagtgatgaa gtggctgcgg ataccgttcg taagacactg
     8521 ctcacccagg ttgcacctcc gggcgtaaca gcacacgtag ttgatgttgc caaaatgatt
     8581 cgcgtctaca acaacccgaa atatgctggc gaacgcgtaa tgctgttatt taccaaccca
     8641 acagatgtag agcgtctcgt tgaaggcggc gtgaaaatca cctctgttaa cgtcggtggt
     8701 atggcattcc gtcagggtaa aacccaggtg aataacgcgg tttcggttga tgaaaaagat
     8761 atcgaggcgt tcaagaaact gaatgcgcgc ggtattgagc tggaagtccg taaggtttcc
     8821 accgatccga aactgaaaat gatggatctg atcagcaaaa tcgataagta acgtattgtg
     8881 ttgattatca ctcagttttc acacttaagt cttacgtaaa caggagaagt acaatggaga
     8941 ttaccactct tcaaattgtg ctggtattta tcgtagcctg tatcgcaggt atgggatcaa
     9001 tcctcgatga atttcagttt caccgtccgc taatcgcgtg taccctggtg ggtatcgttc
     9061 ttggcgatat gaaaaccggt attattatcg gtggtacgct ggaaatgatc gcgctgggct
     9121 ggatgaacat cggtgctgcc gttgcgcctg acgccgctct ggcttccatc atttctacta
     9181 ttctggttat cgcaggtcat cagagcattg gtgcaggtat cgcactggca atccctctgg
     9241 ccgctgcggg ccaggtactg accatcatcg ttcgtactat caccgttgct ttccagcacg
     9301 ctgcggataa ggctgctgat aacggcaacc tgacagcgat ttcctggatc cacgtttctt
     9361 ctctgttcct gcaagcaatg cgtgtggcta ttccggccgt catcgttgcg ctgtctgttg
     9421 gtaccagcga agtccagaac atgctgaatg cgattccgga agtggtgacc aatggtctga
     9481 atatcgccgg tggcatgatc gtggtggttg gttatgcgat ggttatcaac atgatgcgtg
     9541 ctggctacct gatgccgttc ttctacctcg gcttcgtaac cgcagcattc accaacttta
     9601 acctggttgc tctgggtgtg attggtactg ttatggcagt gctctacatc caacttagcc
     9661 cgaaatacaa ccgcgtagcc ggtgcgcctg ctcaggctgc tggtaacaac gatctcgata
     9721 acgaactgga ctaacaggtg agcgaaatgg ttgatacaac tcaaactacc accgagaaaa
     9781 aactcactca aagtgatatt cgtggcgtct tcctgcgttc taacctcttt cagggttcat
     9841 ggaacttcga acgtatgcag gcactgggtt tctgcttctc tatggtaccg gcaattcgtc
     9901 gcctctaccc tgagaacaac gaagctcgta aacaagctat tcgccgtcac ctggagttct
     9961 ttaacaccca gccattcgtg gctgcgccga ttctcggcgt aaccctggca ctggaagaac
    10021 agcgtgctaa tggcgcagag atcgatgacg gtgctatcaa cggtatcaaa gtcggtttga
    10081 tggggccgct ggctggtgta ggcgacccga tcttctgggg aacggtacgt ccggtatttg
    10141 cagcgctggg ggctggtatc gcgatgagcg gcagcctgtt aggtccgctg ctgttcttca
    10201 tcctgtttaa cctggtgcgt ctggcaaccc gttactacgg cgtagcgtat ggttactcca
    10261 aaggtatcga tatcgttaaa gatatgggtg gtggcttcct gcaaaaactg acggaagggg
    10321 cgtctatcct cggcctgttt gtcatggggg cattggttaa taagtggaca catgtcaaca
    10381 tcccgctggt ggtctctcgc attactgacc agacgggcaa agaacacgtt accactgtcc
    10441 agactattct ggaccagtta atgccaggcc tggtaccact gctgctgacc tttgcttgta
    10501 tgtggctgtt gcgcaaaaaa gttaacccgc tgtggatcat cgttggcttc ttcgtcatcg
    10561 gtatcgctgg ttacgcttgc ggcctgctgg gactgtaaga ctgttgtaca ctaccggggc
    10621 cttttggccc cgttttttta tctggaggat taatgacaat cacggacctg gtactgattc
    10681 ttttcatcgc cgcacttctg gccttcgcga tctacgatca gttcatcatg ccccgccgta
    10741 acggccccac cctgctggca attcctttgc tccggcgtgg tcgcatcgat agcgttatct
    10801 tcgtcggatt gattgtcatt cttatctata acaacgtcac gaatcatggc gcgcttataa
    10861 caacatggtt attaagtgca ctggctctga tgggttttta tatattctgg attcgcgttc
    10921 cgaagatcat ctttaaacaa aaaggttttt tcttcgccaa tgtctggatt gaatatagcc
    10981 gaatcaaagc gatgaacttg tcggaagatg gcgtgttggt gatgcaatta gaacagcgtc
    11041 ggctgttaat ccgcgttcga aatatcgacg atttggaaaa aatttataag cttctcgttt
    11101 caactcaata agttatgaat ttagccaaag ctatgtttag tgtattttta ataatcatac
    11161 atagcttagg ctatattagc tcttccctta tttgtta
//
LOCUS       DACADF010000144        11126 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.146, whole genome shotgun sequence.
ACCESSION   DACADF010000144 DACADF010000000
VERSION     DACADF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.146"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..350
                     /locus_tag="ISB30_003844"
     CDS             <1..350
                     /locus_tag="ISB30_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HAP1991433.1"
                     /translation="LFGTYKWFDFEVMALKPLISPTWLNILYIMFGVHGGSYFLGIVE
                     TITFLALSIGFFRPAAGILGDIIVIVTGLVTLSLLPQLGKVDSFVIKDVLLLGAGSVL
                     LKYDLKNYLNIKN"
     gene            complement(493..706)
                     /locus_tag="ISB30_003845"
                     /pseudo
     CDS             complement(493..706)
                     /locus_tag="ISB30_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1068..2261)
                     /locus_tag="ISB30_003846"
     CDS             complement(1068..2261)
                     /locus_tag="ISB30_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1991434.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPCWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2397..4121
                     /gene="iucA"
                     /locus_tag="ISB30_003847"
     CDS             2397..4121
                     /gene="iucA"
                     /locus_tag="ISB30_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAP1991435.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4122..5069
                     /gene="iucB"
                     /locus_tag="ISB30_003848"
     CDS             4122..5069
                     /gene="iucB"
                     /locus_tag="ISB30_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAP1991436.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNLR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5069..6811
                     /gene="iucC"
                     /locus_tag="ISB30_003849"
     CDS             5069..6811
                     /gene="iucC"
                     /locus_tag="ISB30_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAP1991437.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            6808..8085
                     /gene="iucD"
                     /locus_tag="ISB30_003850"
     CDS             6808..8085
                     /gene="iucD"
                     /locus_tag="ISB30_003850"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAP1991438.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            8167..10368
                     /gene="iutA"
                     /locus_tag="ISB30_003851"
     CDS             8167..10368
                     /gene="iutA"
                     /locus_tag="ISB30_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAP1991439.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSDYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2666 a         2905 c         2897 g         2658 t
ORIGIN      
        1 tgctcttcgg gacctataaa tggtttgatt ttgaagtgat ggccctcaag cccctgattt
       61 ctcccacgtg gctgaatatc ctgtacatta tgtttggtgt tcatgggggg agctatttcc
      121 tcggcatagt agaaactatc acttttctag cactgagtat cggcttcttc aggcctgctg
      181 ctggcatact gggagatatt atcgttattg ttacaggttt agtcacacta agtctgttac
      241 cacaactggg aaaagttgac agttttgtca tcaaagatgt gctgctgtta ggtgctggta
      301 gtgtcctgct gaaatatgat ttgaaaaatt atttaaatat caaaaactga tttttcactt
      361 cggtgatgaa cgtttgcgta ttttcatgga aagagattac ctaatccccc tcgttctcct
      421 tcatgttaat acgcaacgtt atggaaaaca aaaataccct gagtaaaaag gtccgtaggc
      481 aggcgagtac ttttattttc cgctagtttt actttcccat cgaaggcccc atacatcact
      541 acgcccacga aacaaccgac gaaataacgc aactttctca tccgtggata atacggaaac
      601 gcactgcaca ggactctgcg gtttacgtcg ccattcaatc ccataagctt caagtgatgt
      661 cttccctcag tgctgctaat tcatcactgt cagatttatg aaccatattc gttacctgta
      721 tccgaaccac tgaagcacca cagcagaaat tcgggtcccc tacactgatt acagcgcccc
      781 ctctgctgcc tattaactca agcacctccc aacaacttac ggaaacttaa ttctaataca
      841 gctgtaattc agttgtgtcg tggtcggccg cggttacttt tttcgggaag tctgctacgc
      901 atcagaataa taccgtgttc tttctgatcc tgtttagcca ggtagctaag ggcgtaacgt
      961 aaaccaggcg ggggcagcaa gccagaacac tgcctgacat gttttcatca gacgcaggtc
     1021 ctctctcata tcctctgatt tactccagta aggtgatgat catatcttta tttgatagta
     1081 cggtaatgcc agataaatac agacacaatg gtaaataata tagttgcaaa taatgctgcc
     1141 ataatcagcg cgattgcgac acctgttttc gataccagcg gcaccagcaa agcgcacagt
     1201 ccatagccaa gtgtgtgact ggtagcgagc cagcccgcgc ctgcgccatc agccagctta
     1261 tcattcagta gaagttgata agcgggtgtt gccagagctg ctccaaatga cagcactgca
     1321 cagcccgggt aaaacaacca tagctgttcg gagagcatga tagccagacc accactcatc
     1381 aacactccgg cactcaggag cagggccacc ggagtcaggc gctgcggacg gagtaccccg
     1441 aactgcgcga taagcgcagc tactgcggac agacccaaca gccaggccac ctgttggcta
     1501 atggcggtgg tatccgtggc gaactggcga gtaagggcag gcgaaagtcc aagctgcatc
     1561 atgctcaccg ccgcagccag taatattgcg caaagcagat aaggcagaca atcccgcttc
     1621 agactgacgc tcttgcactc cggtgtgggc tgtggtggtg tgccgggcaa ccgcagcagc
     1681 atcagcagcg ccagcactgg ggctgccatc agcagcccca gtggcgccag aggatgaatg
     1741 gccagcattg ctgccgcgca cagcggcccg aatagcctgc cgcaactcag gccggagctg
     1801 atggttgcca gagcggccat gcgattccct tctccagctc tctgcaatgc ccagacctga
     1861 caggctggca ccattgccga cacggtcagc ccgtaggcga tccgggcgat gattaatatt
     1921 cccagtccca ccgctgttgt tatggcgctg gtagccatca gcacgcttcc cagccctagc
     1981 agggtaaagc ttatgccata tcctcccaga gcccacaaca ccacaggttt actgccagta
     2041 cgggaaattt gctttcccca gcacggggac gatggcagaa acagcattga gccaagcatc
     2101 agcagggcag cccatacaga cagactcaga tttgtctgta tgaccagaac agggagcaca
     2161 accagcagac cgttctgtcc gataccgaga agcccggcac tgaacgcaag tggccagcag
     2221 gacagtggtt tttggggcgt atcttcgatc cctggtgaca ttataatgtt tcaactccat
     2281 gtattaattg tgtttatttg taaaattaat ttatctgaca ataacattta ttattgataa
     2341 tgagaatcat tattgacata attgttatta ttttgctgtg tgggagctgt ttgactatga
     2401 ccctgccctc tgaaaaacca gccacagatg tggctgcgca gtgcttcctg aatgcactga
     2461 ttcgtgaaac cacagactgg aaactgacag aatacccgcc agacgaattg ctcatcccgc
     2521 tggatgagca gaaatcgctc catttcagag tggcttattt ctccccaacc cagcatcacc
     2581 gctttgcatt tcctgcacgt ctggtcacgg catcaggcag ttatcctgtc gactttacca
     2641 ctctctcccg gcttattatt gataagctac gccatcaact gtttctgccc gttcccctct
     2701 gcgaaacttt ccaccagcgc gtgctggaaa gccatgtcca tacgcaacag gcaattgatg
     2761 cccgtcatga ctgggccgct ctgcgtgaaa aagcgttgaa ttttggcgag gctgagcagg
     2821 cgctgctgac aggacacgct ttccaccctg cgcctaagtc tcatgaaccg tttaaccggc
     2881 gggaggctga acgctacctg cctgatatgg caccccactt cccactgcgg tggttttcgg
     2941 tggataaaac gcaaatcgcc ggtgaaagtt tgcatctcaa ccttcaacag cggctgacgc
     3001 gatttgccgc agagaatgcg cctcagttac tcaacgaatt aagtgacaac caatggctgt
     3061 tcccgttgca cccgtggcag ggggaatatc ttttgcagca ggggtggtgc caggcacttg
     3121 ttgctaaagg gctgattaaa gacttaggtg aggccggcac gtcgtggctg ccgaccacct
     3181 cttcccgttc cctctactgt gccaccagcc gcgatatgat caagttctcc ctgagcgtac
     3241 ggctgaccaa ctccatccgt actctgtccg tgaaagaagt gaagcgtggg atgcgcctgg
     3301 cacgcctggc tcaaaccgac ggctggcaga tgctacaggt ccgcttcccg actttccggg
     3361 taatgcagga ggatggctgg gccgggctgc gcgatcttaa cggcaacatc atgcaggaaa
     3421 gtctgtttgc cctgcgtgaa aatctgctgg tggatcagcc gaaaagccag actaacgtac
     3481 tggtctccct gactcaggcc gcacctgatg gcggtgattc gctgctggtt tcggcggtaa
     3541 aacgcctgag cgatcgcctc ggtatcactg tgcaacaggc cgcccatgca tgggtcgatg
     3601 catactgtca gcaagtgcta aagccgctgt ttacggctga agcggattac ggcctggtgc
     3661 tgctggcgca tcagcaaaat attcttgtcc agatgcttgg ggatctgccg gtcggattta
     3721 tttaccgtga ctgtcagggc agcgctttta tgcctcacgc gacagactgg ctcgattcta
     3781 ttggcgaggc gcaggcggaa aatattttca cccatgagca gttgctgcgc tatttccctt
     3841 attacctgct ggttaactcc acttttgctg tgaccgctgc gctgggggct gccgggctgg
     3901 acagcgaatc gaatctgatg gctcgtgtac gagcatcgct ggctgaagtg cgtgatcagg
     3961 tgactcataa aacctgtctc aactacgtgc tggaaagtcc gtactggaac gtaaaaggta
     4021 actttttctg ttatctgaac gatcataacg agaacaccat tgttgaccct tcggtgatct
     4081 atttcgattt cgctaacccg ctgcaggctc aggaggtctg aatgtctgag gcaaacatta
     4141 ttcacagcag atatggactg cgctgtgaaa aactcgacaa gcccctgaat cttggctggg
     4201 gactggacaa tagcgcggtg ttgcactgtc ccggggagct gccgacaggg tggctgtgcg
     4261 atgcgctgga tcagatattt atcgccgctc cacaactgtc agcagtggct ctcccctggg
     4321 ccgaatggcg tgaggagcca caggcgctga cgctttttgg gcaggtaaaa agcgacatta
     4381 tccatcgtac tgccttctgg cagttaccgt tatggttgag ttcaccggca aaccgggcct
     4441 ccggtgaaat ggtttttgat gcagagcgtg agatttattt cccgcagcgc ccaccccgtc
     4501 cacagggtga ggtttaccgc cgttacgatc cacgtattcg caggatgctg agtttccgca
     4561 ttgccgatcc cgtttctgat gcagaacgtt tcacccgctg gatgaatgat ccgcgcgttg
     4621 agtatttctg ggagcaaagt ggctcactgg aggtacagac cgcctatctg gagcgccagc
     4681 tcaccggtaa acatgcattc ccgctgattg ggtgcttcga cgatcggcca ttcagctatt
     4741 tcgaaatcta ctgggcggcg gaagaccgca ttggtcgtca ctattcgtgg cagccctttg
     4801 accgtggcct gcatctgctg gttggtgaac agcaatggcg cggggcccac tatgtgcaaa
     4861 gctggctgcg cgggctgaca cattacctgc tgctggatga gccccgtacg cagcgcacag
     4921 tactggagcc acgcaccgat aacctgcgcc tgttccgcca tcttgagcct gcgggatacc
     4981 ggacaattaa agagttcgac ttcccacata agcgctcgcg catggtgatg gcggatcgtc
     5041 atcacttctt cacggaggtc ggtctgtaat gaatcacaag gactgggatt ttgttaaccg
     5101 tcggctggtg gcaaaaatgt tgtctgagat ggagtatgag caggttttcc acgcggaatc
     5161 tcagggcgat gaccactact gcattaacct gccgggagca caatggcgct tcatcgctga
     5221 acgtggtatc tggggctggc tgtggattga tgcccaaacg ttgcgctgca cggacgaacc
     5281 agtactggcg cagacgctgc tgatgcagct aaagccggtg ctgtcaatga gtgatgccac
     5341 tgttgcagag catatgcagg atttgtatgc cacgctactg ggcgacctgc aactgctgaa
     5401 ggcccgtcgc ggactgagtg ccagtgacct gattaatctt gatgccgatc gtctgcaatg
     5461 cctgctgagc ggtcatccta aattcgtttt taataaaggt cgccgtggct ggggcaaaga
     5521 ggcgctggaa cgatatgcgc cagagtatac caacaccttc agactgcact ggctggcggt
     5581 aaaacgtgaa catatgatct ggcgctgcga taacgatctg gatattcagc agttgttgac
     5641 tgccgcaatg gatccgcagg agtttacacg cttcagtcag gtctggcagg aaaacggact
     5701 ggatcacaac tggctgccgc tgccggtaca tccgtggcag tggcagcaaa aaatcgctac
     5761 cgacttcatc gctgattttg ccgaaggcag gatggtgtct ctcggcgagt ttggcgacca
     5821 gtggctcgcc cagcagtcgc tgcgtaccct gaccaacgcc agtcgccggg gcgggctgga
     5881 tatcaagctg ccgctgacca tctacaacac ctcatgctac cgggggatcc ctggcagata
     5941 catcgctgcc ggaccactgg cttcacgctg gctacaacag gtttttgcga ccgacgccac
     6001 cctggtgcaa agcggcgcag tgatacttgg tgaaccggct gcaggctatg tctcccatga
     6061 aggctatgcc gcgcttgccc gggctcccta tcgctaccag gaaatgcttg gtgttatctg
     6121 gcgggagaat ccgtgccgct ggctgaaacc ggatgaaagt ccggttctga tggcaacact
     6181 gatggagtgc gacgaaaaca atcagccgct ggcaggcgca tatatagacc gctccgggct
     6241 ggacgctgaa acctggctta cgcaactgtt ccgggtggtc gtggttcctc tgtatcacct
     6301 gctttgccgc tacggtgtcg cgcttattgc acatggacaa aatataactc tcgccatgaa
     6361 agagggggtt ccacagcgtg ttctgctgaa agacttccag ggcgatatgc gactggtgaa
     6421 agaagagttc cccgaaatgg actctttgcc tcaggaggtt cgtgatgtta catcccgcct
     6481 gagtgcggac tacttaatcc atgatttgca gacgggtcac ttcgtgacag tactgcgttt
     6541 tatttcgcca ctgatggttc gtcttggcgt acctgaaagg cgattttatc aactgctggc
     6601 agcagtgttg agtgattaca tgaaaaaaca tccacaaatg tcagagcgtt ttgcgctttt
     6661 ctcacttttc aggccacaaa tcattcgcgt ggtgctgaac ccggtaaaac tgacctggcc
     6721 tgatctggat ggcggcagcc gcatgctgcc gaattacctt gaggatctgc aaaatccgct
     6781 gtggctggta actcaggaat atgaatcatg aaaaaaagtg tcgattttat tggtgtaggg
     6841 acagggccat ttaatctcag cattgctgcg ttgtcacatc agatcgaaga actggactgt
     6901 ctcttctttg atgaacatcc tcatttttcc tggcatccgg gtatgctggt accggattgt
     6961 catatgcaga ccgtctttct gaaagatctg gtcagtgctg ttgcacctac aaatccctac
     7021 agttttgtta actatctggt gaagcacaaa aagttctatc gcttccttac aagcagacta
     7081 cgtacagtat cccgtgaaga gttttctgac tacctccgct gggctgctga agatatgaat
     7141 aacctgtatt tcagtcatac cgttgaaaac attgatttcg ataaaaaacg tcgattgttt
     7201 ctggtgcaaa ccagccaggg agaatatttt gcccgcaata tctgccttgg tacaggaaaa
     7261 caaccttatt taccaccctg tgtgaagcat atgacacaat cctgtttcca tgccagtgaa
     7321 atgaatcttc gtcggccgga ccttagtgga aaacggataa ccgtggttgg tggaggacag
     7381 agtggtgcag acctgttcct taatgcatta cgcggggaat ggggagaagc ggcggaaata
     7441 aactgggtct cccggcgtaa taattttaac gcactggatg aggctgcttt tgctgatgag
     7501 tattttacac ctgaatatat ttcaggcttc tccggactgg aggaagatat tcgccatcag
     7561 ttactggatg agcagaaaat gacatcggat ggcatcactg ccgattcttt actgaccatt
     7621 tatcgtgagt tgtaccaccg ttttgaagtt ctgagaaaac caagaaatat ccgtctgcta
     7681 cccagccgct cggtaacaac tctggaaagt agtggtcctg gctggaagtt gctgatggag
     7741 catcatctgg atcagggcag ggagagcctg gaaagtgatg tggtgatttt cgccacaggt
     7801 taccgttctg cgttgccaca aatacttccc tcactgatgc ccctgatcac catgcacgat
     7861 aagaacacct ttaaagtgcg tgatgacttc actctggaat ggagtggccc gaaagagaac
     7921 aacatcttcg tggtcaacgc cagtatgcaa acccatggca tcgccgaacc ccagctcagc
     7981 ctgatggcat ggagatctgc acgtattctt aatcgcgtaa tgggacgtga tttattcgat
     8041 ctcagtatgc cgcccgccct gattcagtgg cgcagcggca cctagggaaa acgcagccgg
     8101 aggctgcttc tttaactcgc tacacagcat ctttgggctg attttttccg cccgtatgga
     8161 ggaataatga tgataagcaa aaagtatacg ctttgggctc tcaacccact gcttcttacc
     8221 atgatggcgc cagcagtcgc tcaacaaacc gatgatgaaa cgttcgtggt gtctgccaac
     8281 cgcagcaatc gcaccgtagc ggagatggcg caaaccacct gggttatcga aaacgccgaa
     8341 ctggaacagc agattcaggg cggcaaagag cttaaagacg cactggctca gctgatccct
     8401 ggccttgacg tcagcagccg gagccgcacc aactacggta tgaatgtgcg tggccgcccg
     8461 ctggtcgtgc tggttgacgg cgtgcgtctc aactcttcac gtaccgacag ccgacaactg
     8521 gactctatag atccttttaa tatcgaccat attgaagtga tctccggtgc gacgtccctg
     8581 tacggcggcg gcagtaccgg tggcctgatc aacatcgtga ccaaaaaagg ccagccggaa
     8641 accatgatgg agtttgaggc tggcaccaaa agtggcttta gcagcagtaa agatcacgat
     8701 gaacgcattg ccggagctgt ctccggcgga aatgagcata tctccggacg tctttccgtg
     8761 gcatatcaga aatttggcgg ctggtttgac ggtaacggcg atgccacctt gcttgataac
     8821 acccagaccg gcctgcagta ctccgatcgg ctggacatca tgggaaccgg tacgctgaac
     8881 atcgatgaat cccggcagct tcagttgatc acacagtact ataaaagcca gggcgacgac
     8941 gattacgggc ttaatctcgg gaaaggcttc tctgccatca gagggaccag cacgccattc
     9001 gtcagtaacg ggctgaattc cgaccgtatt cccggcactg agcggcattt gatcagcctg
     9061 cagtactctg acagcgcttt tctgggacag gagctggtcg gtcaggttta ctaccgcgat
     9121 gagtcgttgc gattctaccc gttcccgacg gtaaatgcga acaaacaggt gacggctttc
     9181 tcttcgtcac agcaggacac cgaccagtac ggcatgaaac tgactctgaa cagcaaaccg
     9241 atggacggct ggcaaatcac ctgggggctg gatgctgatc atgagcgctt tacctccaac
     9301 cagatgttct tcgacctggc tcaggcaagc gcttccggag ggctgaacaa caagaagatt
     9361 tacaccaccg ggcgctatcc gtcgtatgac atcaccaacc tggcggcctt cctgcaatca
     9421 gactatgaca tcaataatct ctttaccctc aacggtggcg tacgctatca gtacactgaa
     9481 aacaagattg atgatttcat cggctacgcg cagcaacggc agattgccgc cgggaaggct
     9541 acatccgccg acgccattcc tggcggctca gtcgattacg acaacttcct gttcaacgcc
     9601 ggtctgctga tgcacatcac cgaacgccag caggcatggc tcaacttctc ccagggcgtg
     9661 gagctgccgg acccgggtaa atactatggt cgcggcatct atggtgctgc agtgaacggc
     9721 catcttcctc taacaaagag tgtgaacgtc agcgacagca agctggaagg cgtgaaagtc
     9781 gattcttatg agctgggctg gcgctttact ggcaataatc tgcgcaccca aatcgcggcc
     9841 tactattcga tttctgataa gagcgtggtg gcgaataaag atctgaccat cagcgtggtg
     9901 gacgacaaac gccgtattta cggcgtggaa ggtgcggtgg actacctgat tcctgatact
     9961 gactggagta ccggagtgaa cttcaacgtg ctgaaaactg agtcgaaagt gaacggtacc
    10021 tggcagaaat acgatgtgaa gacagcaagc ccatcaaaag cgacagccta cattggctgg
    10081 gcaccggacc cgtggagtct gcgcgtgcag agcaccacct cctttgacgt gagcgacgcg
    10141 cagggctaca aggtcgatgg ctataccacc gtggatctgc tcggcagtta tcagcttccg
    10201 gtgggtacac tcagcttcag cattgaaaac ctcttcgacc gtgactacac cactgtctgg
    10261 gggcagcgtg caccactgta ctacagcccg ggttacggcc cagcgtcact gtacgactac
    10321 aaaggcaggg gccgaacctt tggtctgaac tactctgtgc tgttctgacg gggatgcttt
    10381 tgcaacaagg gtatgctgta aacaacatgg tccttggtgg ccatgttgtt tacagccagt
    10441 ataatataca gccttaacaa gatattccgt ctccggaatg gactgttcac cactgaataa
    10501 cattatgtct gttttatgga tctttgactg aaatttatcg catatcctca tttcattgtc
    10561 cgtctgaaaa ctaaccttac aagtaagcgt cgtctcagca ccgtctggca gatcctgaaa
    10621 ttcctgggag gatgatctgc gcccaatatc ctgagccgga actttgctgc aatgaaggcc
    10681 aacgaaaaat gggggagtat gttatagagt acttgctaca gggtaaaaag aggtacctgt
    10741 cgctggttct ccgttcagtt taaccgggag attttctcct gcgcctgtca taaagtccgg
    10801 taatggagat tgttaatacg atggtgcacg atgcattgtc agtgcccggc ccggaggatg
    10861 ccccgtagct acatacggat cagggttggc aatatcgaat ggcaggctat aggcaaagtt
    10921 aaagccccat ggagtagcac aaaatatgcc gcacaaagga aacggtctga ataacgcagc
    10981 gatggagaac ttcttcggca cactgcaaaa tcgcatcgtg gtcgatcgct aattcgtcgt
    11041 gaatcactga acgatgttcc atgcgaacag atattcgatc atatcgaaat attaaacatt
    11101 acccctcccc acattgggca aaaaaa
//
LOCUS       DACADF010000145        11102 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.147, whole genome shotgun sequence.
ACCESSION   DACADF010000145 DACADF010000000
VERSION     DACADF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.147"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..1815)
                     /gene="selB"
                     /locus_tag="ISB30_003852"
     CDS             complement(<1..1815)
                     /gene="selB"
                     /locus_tag="ISB30_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAP1991440.1"
                     /translation="MIITTAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRIVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLL"
     gene            complement(1812..3203)
                     /gene="selA"
                     /locus_tag="ISB30_003853"
     CDS             complement(1812..3203)
                     /gene="selA"
                     /locus_tag="ISB30_003853"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAP1991441.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQALLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3301..3909)
                     /locus_tag="ISB30_003854"
     CDS             complement(3301..3909)
                     /locus_tag="ISB30_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAP1991442.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3980..5116)
                     /locus_tag="ISB30_003855"
     CDS             complement(3980..5116)
                     /locus_tag="ISB30_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP1991443.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLEPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5119..5481)
                     /locus_tag="ISB30_003856"
     CDS             complement(5119..5481)
                     /locus_tag="ISB30_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAP1991444.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6018..7931
                     /locus_tag="ISB30_003857"
     CDS             6018..7931
                     /locus_tag="ISB30_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP1991445.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAAARRVQEMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8251..9399
                     /gene="mtlD"
                     /locus_tag="ISB30_003858"
     CDS             8251..9399
                     /gene="mtlD"
                     /locus_tag="ISB30_003858"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP1991446.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9399..9986
                     /gene="mtlR"
                     /locus_tag="ISB30_003859"
     CDS             9399..9986
                     /gene="mtlR"
                     /locus_tag="ISB30_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAP1991447.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9997..10206)
                     /locus_tag="ISB30_003860"
     CDS             complement(9997..10206)
                     /locus_tag="ISB30_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991448.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            10490..10852
                     /locus_tag="ISB30_003861"
     CDS             10490..10852
                     /locus_tag="ISB30_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAP1991449.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         2673 a         2997 c         2831 g         2601 t
ORIGIN      
        1 caataaatga tcatttccac gacgacgcgt aaagccaatg cggtcaaaat attccagaat
       61 ctgaattgcc agcttgcggc ctacgcctaa gcgatcgcgg aaatccgccg cgcaggttga
      121 accacattcc tgatcgagat cgcggatcat attggcaaat tcgacaatcc gatcgttacg
      181 gtaataacga tctttgacga tcgcagtaat tatcccctgt tgggccgcct ggcgtagcgt
      241 caggcgcatt gcctgctcgt cggttcccgt ctcttttgcc aggtcacgca cccaccatgg
      301 ctcgtcacca aacagcggct ctgctttttg ccagatggcc tgctgctctt cgctgaagcc
      361 cgctttatga tccggcagat gcagccagcc gtgatggctg tggatgtcgc cgctttcgcg
      421 catcttttca atcagcaaca gtaccagcgc ttcatcttcc attggcaacg ccatacgtcg
      481 cagacgttcg cgcccaggac caggttcatc gcgatgttgc tcatgataag tcgctaatgt
      541 gtcgagaatt ttccgctgcc agcgggcggc aactggcgca ttcaacaagc tgtaaccagc
      601 ctgaatatag ccaggctgtt gcagcagttc gcgcatccct tcgccgttga gctgccgtgc
      661 ccaggcgaaa tccgcaaggt taaccgcgcc gcgttccaga tgaacagata acgcatcggc
      721 atcgctctgc gcccgtgcaa gagacgccag ccattgcaga tattccggct tacgcttacc
      781 gcgacgcggc gggttaagca tcaccacacg cgcccccgcc agcgtgttgc gggccgagat
      841 atcgcgcagc actatgcgat cgttatccgc cagccacagc ggcgtgtcga acaccagctc
      901 ggcaaggtta tcttccagta gcgaaacacg tcccgtaacg tggctggcgg cgtggtgaat
      961 atgcagcggc tgccactggg tcagcggtgt atgggtttga agctcgacaa tcacccgtgt
     1021 gaacggctcc ggcggcacat cggcaagcag ccagtcgcca cggttaatct gctctttttc
     1081 cgcatcaccc gcgatgttaa gcgcgatacg ctgcccggca tgggcagttt ctgttggctg
     1141 attttgcgca tgcagcgcac gcacgcgcat cggtttattt acaccagtca gccagagtga
     1201 atcgcctact ttcacttccc cgcttaacgc cgtaccggtg acgaccagcc cggcaccttt
     1261 tacggtaaat gcgcggtcga tcgcgaggcg gaaactatgt tggctggcgt gttcacgttc
     1321 tggtaactga agcagatgct cgcgcagggc atcaattccc cgaccttcgg ttgctgcggt
     1381 gataaacagt tttgcctcag caaaaccgta ttcccgcagc acctccttta cctggcgttc
     1441 aacctcatca acacgcgctt cgtccacgcg atcggctttg gtcagcgcca ctgtcagcat
     1501 cgggttaccg gtcagctgca aaatcgccag atgctcacgg gtctgtgcca tcacaccgtc
     1561 atcgcacgcc accaccaaca gcgcgtgatc gataccacca acgcccgcca gcatgttgga
     1621 aagaaacttt tcatgaccgg gtacgtcgat aaaaccaggc acgcgaccat ccggctgcgg
     1681 ccagtaggca tagccgagat cgatggtcat gccgcgcttt ttttcttccg gcagacggtc
     1741 ggcatttacg cccgtaatcg cctgcaacaa ggttgttttg ccgtggtcaa cgtgtccggc
     1801 agtcgtaata atcatttcaa caacatctcc aaaaaccgtt gctcatcttc aaggcagcgt
     1861 aaatccagcc acaatcgtcc gtcataaata cgaccaatca ccggcactgg caattcacgc
     1921 cagcgggcgg ctaatgactc aaggtggcta ccgcgtccat catggggggt aaacgttaat
     1981 gccgcgctcg gcaggcgatc aaccggcagc gaaccactgc cgatctgcga aagacatggc
     2041 ataacctgta ccgcaaactc cgcgccgtaa tgtgcggcaa ggagagcctg taaacgttgt
     2101 gcctggattt gaatgacctc tgcgctgcgg gtaagcaggc gcagggtcgg taatttttca
     2161 ctcagagctt cagggtgtaa ataaagacgc aacgtggctt ccagcgccgc gagggtcatt
     2221 ttatccgcgc gtaatgcacg cttcagcggg tggctttgca ggcgggcgat catttctttt
     2281 ttaccaacaa taattcctgc ctgcggcccg cctaacagct tgtcgccgga gaagctcacc
     2341 agactgacgc ccgccgcaat caactcctgc ggcattggct ctttcggcaa accgtactgg
     2401 ctaagatcga ccagcgagcc actgcctaaa tcagtcacca ctggaacatc cagttctttg
     2461 ccgagcgcca ccagttccgc ttcatctatc gctttggtga acccctgaat gctgtagtta
     2521 ctggtatgta ctttcatcaa cagtgcggta ttttcattca ccgcctgacg ataatcattc
     2581 gcgtgcgtgc ggttggtggt ccctacttcg tgtagggtgc atcctgcctg acgcataaca
     2641 tcgggaatac gaaacgcgcc gccaatctcc accagttcgc cgcgagaaac caccacctct
     2701 ttcccgctgg cagtggccgc caacatcaat aacaccgccg ccgcattgtt attaacgata
     2761 caggcatctt ccgcccccgt aatacggcac agcagctgcg ccagcgcccg atcgcgatgt
     2821 ccacgtccgg cgtcgtccag atcgtactcg agggtcactg gcgaacgcat agccttcgta
     2881 acggcttcca ccgcggcttc cgcctgtaaa gctcgcccaa ggttggtatg cagcacggtt
     2941 cccgtcaggt tgatcaccgg acgcagcgcg ctttgcgcct ctttcgtcaa ccgggcatcg
     3001 acttcttgcg cccagttttc acaccacgcg ggcagcgtct ggctgtcacg aatcacttct
     3061 cgcgcttcgt caagcatctg acgcagcaat tccaccacgc gggtgtgacc ataagtatca
     3121 cgcaaagaaa ggaaggagct atcgcgcaat aagcgatcaa tagccggaag ttgactatag
     3181 agggaacgcg tttcggttgt cataggaaac ctggctgatc aaggccctct cacacggaga
     3241 agggcgttta acataaccac ggattgtaac gtgagatggg tcaggaggac atatcgcgca
     3301 tcaagccttt ggcggttcgg tgcgagcaaa actttcgcgg ctaaacaggt tttcgaccag
     3361 tttgactaag tgtgggcggt cgacacacca gcctggcgca acgcggcgga aattgagata
     3421 tccgaccgca caggcaatgg cgatagtcgc cagattgacc gtatcggttt tgagcgtgcc
     3481 atcgaccaga tatccttcca gcacatccag actgcggttg attttctcac gctgacgtaa
     3541 caattcatct tcagactgct gcgccgccgg acgcgcctgt tcacgcaccg ataccagccc
     3601 ggcatccata atgccatccg ccagcgcctc aattttgcgc acccgcaacg actccagcgg
     3661 atcacgcggc aacatcgccg gagcaacatt cattaattca atatattcag cgatgatcgg
     3721 cgaatcaaac cagcattcgc cctcttcggt caccagcacc ggcacttttc ctaacgggtt
     3781 aaattgcgcc acgccgttgt ccgcgttata gggcagttca ttaataaatt cgaacgttat
     3841 gcccttttct aacaacagaa tagaaagttt gcgtacaaac gggctggtgt agctaccgac
     3901 gagtttcatg ccgagtcctt tgtgcgagga aaaatatcag tatggctcag gctcgcgcaa
     3961 aacacaggtg atacaagtct caatgatcca aataaagata atgcatccaa ctggtcattc
     4021 ttagcagcac tttccgcatc accgaaacat ggctgaaatg gtcggagtaa actgccacct
     4081 gggcgtagat gccgtcgggt aaggcatcga tatcagcgtt agggtccagc tcaatggttc
     4141 ccagcacacc gtccgtgccg ggcacgaccg ttaatgattg caataccccc tgcgcctgat
     4201 aagaaccgcc tggcacgaca ggtaaaatac tggtcagttt gccgtgaaac acctgcccag
     4261 gtagcgcgtt aaacaccact tccgcatcat cgccgggttt cagacgtaac agcgagtttt
     4321 gccgaaattg ggcgacaatt tgccgttttt gctcggggat gaagaccatc accggacgca
     4381 gcggcaaggc agcggcgtat gtacctgggc ggatcagtac ctgagtgacg tagccattgc
     4441 tcggtgcgcg aatgacggtc tgctcaaggt tatattttgc ttcagtaagt tgcgctctta
     4501 agctcacaat ctgcgattgc tcgccgttaa ccatactgtc gagctggctc tggatctgcg
     4561 cctgctccgc caccgagcct ttcaccagcg catcctgcgc gaggaaattt tgccgcgcat
     4621 cgtcgatgtc acgttccgag aacggattca ccgccgcctg gctgcctttc agataacgtt
     4681 gataattttt aaacagacgg tcgcgctccg ctgaaacctg ggtggtgttg gcctgcgctt
     4741 cagtgagttg cgcgcgcagc gtctttatat tatgcgtcgc cgtcatcagg tctgcctgaa
     4801 gtctgtcaac tcgcgcctgg taacgaaccg gctcgagctt aaaaagcacc tcgcccttct
     4861 gaataagctg attatttttg tcggtgactt cagtaacaat tcccgtcacc tgtggcgtga
     4921 tggggatcgc tatcactgcc ttttgcgcgg taaaagtgta agggtggttg tagttcatca
     4981 acaagatcaa accactcacc agaaacacgc ctcccagcgc cgccgtcgcc agcgtccact
     5041 gatttaccgg aatgcggaag attttaaaga ccgcccacgc cagcgccacg taagttaaaa
     5101 caatcaatag atccatattt agatctccgg aaacgtggaa tagtcagtct ttttctcggc
     5161 ggcgagttga tgctccagcc gggaaattcg gtcagaaagt gcggcgattt ccgggtcagt
     5221 cgctttctct tgcgacgcaa catgtgactg catgccccag ccgcgctccg gttgatagag
     5281 cgtcgcccag atccacagga acggccagat aacatgtaga gtaaacaggc tcacccagcc
     5341 cgccgtatga atagcgtcgg catggggatg gttgcgcttt ttggcaatca gataagggat
     5401 gtcatgtatc gcgatgattc cataaaaaat caccagaaag acgaagatca gcactcccaa
     5461 cgcgaaatag tttagaaaca tatctgcctc ggattcacgt ttatcagtgt tgttttttgg
     5521 ctggcagcca gaagggagtc aggctgatat tttgacaata atccgggttc gcgattctcg
     5581 ccataacacc aaagaataat tttaagaggt gatgagttgc ttagttacat aacgatttca
     5641 tgacgaaggc ataacatgct gtagatcaca tcaggtgaac gccgtaagaa aatatcttgt
     5701 gattcagatc acaaagattc aacaaaccat caaaacaaaa atgtgacatt actcacattt
     5761 aaatgccatt tttagcgaaa atcgccgcct tgttgctttt ttacacaagc attttgtgat
     5821 gagcatcacg tcaattacct ctctaccccc tacatttatg tgactgatat cacacaaaag
     5881 gccgccgact ggacagttaa ccgattcagt gccagatttc gcagtatcta caaggtccgg
     5941 ctacctctgc cgccacatta acaaaaaacc tcgggcttcc agcctgcgcg acagcaaaca
     6001 taagaagggg tgtttttatg tcatccgata ttaagatcaa agtgcaaagc tttggtcgtt
     6061 tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat cgcgtggggt atcatcaccg
     6121 cgttatttat tccaacaggg tggttaccga acgaaacgct ggcgaagctg gtcgggccga
     6181 tgatcactta tctcctgccg ctgctgatcg gttataccgg tggtaagctg gttggcggcg
     6241 aacgtggcgg cgtagtcggt gccattacca ccatgggcgt tatcgtcggc gcagacatgc
     6301 cgatgttcct cggctctatg attgcaggtc cgctgggcgg ctggtgcatt aagcacttcg
     6361 accgctgggt agacggtaag atcaaatccg gttttgagat gctggtgaat aacttctccg
     6421 caggcatcat cgggatgatc ctcgctattc tggcattcct cggcattggc ccgattgttg
     6481 aagccctgtc caaaatgctg gcggcgggcg ttaacttcat ggttgtccat gacatgctgc
     6541 cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt cctcaacaac gccattaacc
     6601 acggtatctt ctcgccgctg ggtattcagc agtcccatga actgggtaaa tccatcttct
     6661 tcctgattga agctaaccca ggtccaggta tgggcgtgct gctggcgtac atgttctttg
     6721 gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat catccacttc ctggggggta
     6781 tccacgaaat ctacttcccg tatgtgctga tgaatccgcg tctgattctg gccgttatcc
     6841 tcggcggtat gactggcgta ttcacgctga ctatcctggg cggtgggctg gtttctccgg
     6901 catctccggg ttctatcctt gctgtactgg cgatgacgcc aaaaggtgct tacttcgcta
     6961 acatcgcggg tgtgtgtgcg gcgatggcag tttccttcgt tgtctctgcc attctgctga
     7021 aaaccagcaa agtgaaagaa gaagatgata ttgaagctgc tgcacgtcgc gttcaggaaa
     7081 tgaaagctga gtctaaaggt gcgtctccgc tgtctgcagg cgatgtgact aacgacctga
     7141 gccacgtccg taaaatcatc gttgcctgtg atgccggtat gggttccagt gcgatgggtg
     7201 caggcgtgct gcgtaagaaa attcaggatg caggtctgtc gcagatttcg gtcactaaca
     7261 gcgcgatcaa caacctgccg ccagatgtgg acctggtcat cactcaccgt gacctgaccg
     7321 aacgcgctat gcgccaggtt ccgcaggcac agcatatttc gctgaccaac ttcctcgaca
     7381 gcggcctgta caccagcctg accgaacgtc tggttgctgc ccagcgccat actgaaaacg
     7441 aagtgaaagt gaaagacagt ctgaaagaca gctttgacga ttccagtgct aacctgttca
     7501 aactgggcgc ggagaacatc ttcctcggtc gcaaagcggc caccaaagaa gaagcgattc
     7561 gttttgctgg cgagcaactg gtgaaaggcg gttacgttga gccggaatac gttcaggcga
     7621 tgctggatcg tgaaaaactg accccgactt atctgggtga gtctatcgcg gtgccacacg
     7681 gtacggttga agcgaaagat cgcgtactca aaacgggcgt cgtgttctgc cagtacccgg
     7741 aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg tctggtgatt gggattgctg
     7801 cccgtaacaa cgagcacatt caggtgatca ccagcctgac caatgcactg gatgatgagt
     7861 ccgtcatcga gcgtctggca cacaccacca gcgtggatga agtgctggaa ttgctggcag
     7921 gtcgtaagta atccattccc accctctccg catgaagaag gtggggctaa ttgcttgatg
     7981 cgcttcgctt atcaggccta caacataaag cacaatttgt tttgtcggtc ggataaggtg
     8041 tttacgccgc atccggcagt cggtgcacaa tgcctgatgc gacgctgacg cgtcttatca
     8101 gacctacagg tcagtgccaa acacgtaggc cggataaggc ggtttacgcc gcatccggca
     8161 aaaagcccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct
     8221 cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat
     8281 atcggtcgtg gctttatcgg taaactgctg gcagacgcag gtatccaact gacgtttgcc
     8341 gatgtaaatc aggtggtgct tgatgccctg aatgcccgtc atagctatca ggttcatgtg
     8401 gttggtgaaa ccgagcaggt agataccgtt tctggcgtcg atgccgtcag cagcattggt
     8461 gatgatgtcg ttgatctgat tgctcaggtt gatttagtca ctaccgccgt tggcccggtc
     8521 gtgctggaac gtattgctcc ggccatcgcc aaagggctgg tgaaacgtaa agaacaaggt
     8581 aatgaatctc cgctgaacat catcgcctgt gaaaacatgg tccgcggcac cacgcagctg
     8641 aaaggccatg tgatgaacgc cctgcccgaa gacgccaaag cgtgggtaga agaacacgtt
     8701 ggctttgttg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat
     8761 ccgctggaag tgacggtaga aaccttcagc gaatggattg tcgataaaac ccagttcaaa
     8821 ggcacgctgc caaacatccc tggcatggaa ttaaccgaca atctgatggc atttgtcgaa
     8881 cgtaaactct tcaccctgaa tacaggtcat gctataaccg cgtacctcgg aaaactggcg
     8941 ggtcatcaga ccattcgtga cgcaattctc gacgagaaaa tccgcgcggt ggtaaaaggg
     9001 gcaatggaag aaagtggtgc agtattgatc aagcgctacg gctttgacgc tgacaagcat
     9061 gcggcataca tccagaaaat ccttggtcgt ttcgagaacc cgtacctgaa agatgatgta
     9121 gaacgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca
     9181 ctgctcggaa ccctggaata tggtctgcca cataaaaacc tgattgaagg tattgccgct
     9241 gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc gctgattgcc
     9301 gacaaaggcc cacaagccgc gctggcacag atttccggtc ttgatgccaa cagcgaggtt
     9361 gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggata
     9421 ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct
     9481 ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca
     9541 ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag
     9601 acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgatggt ccgctgggcg
     9661 atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg
     9721 aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc aacgagtacg
     9781 cttttaccga cgacgaaatc cttggaccct ttggtgaact gcattgcgtg gcggcgttac
     9841 caccgccgcc acagtttgaa cctgcagact ccagtttgta tgcaatgcaa attcagcgtt
     9901 accaacaggc tgtacgatca acaatggtcc tttcactgac agagctgatt tccaaaatca
     9961 gcttaaaaaa agcctttcaa aagtaagtac tttcgcttac ttctcctcta cctgtttcgg
    10021 acgataaagc tgacgataat actccagacg ttgtagatat aacggcacgc tttcatcagg
    10081 tattccggac ggaatcgcgt tacggggagg tagttttttc agatactccc ggaacgcctg
    10141 gctcgatgcc atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt
    10201 gcccatgatg atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt
    10261 tagatcccgg tataaatgac attaaggaaa acgtgctcaa tcctcaataa tcacctgctt
    10321 ctgttgacta tcttttttct ttaaatttct taactaatca atgtattggg tgatttttta
    10381 acgcattaat ggttacaatc tggaactatt acaatgccat cagttgtgaa caaagcacct
    10441 ggtcgcgcat actagaggct ataaatttat ctctatcaga agtcatcaca tgaaagaagt
    10501 cgaaaaaaac gaaatcaaac gtctcagcga tcgtctggac gccatccgtc accagcaggc
    10561 cgatctatcg ctggttgaag ccgcagacaa atatgccgaa ctggaaaaag agaaagccac
    10621 gctggaagca gaaattgctc gcctgcgcga agttcacagc cagaaactca gcaaagaagc
    10681 gcagaaactg atgaagatgc cgttccagcg tgcaatcacc aaaaaagagc aggctgatat
    10741 gggcaaactg aaaaaaagtg ttcgcggact ggtggttgtg cacccaatga ccgcgctggg
    10801 ccgtgaaatg ggcctgcaag agatgacagg gttctcaaag actgcgtttt aagttccttt
    10861 ctcgtcgctg taactactgc cactcacgat ttactcgctc ctgctggcac atgcaggagc
    10921 ctgtgccact cattaaatac gatgtctttc ttaccgtaaa ctgtttcagt ttcccctcga
    10981 gtttgaggta tttttgcagg ctaaaatctg aaaatttgtt gcgcaacaac aagtgatata
    11041 taaatcataa aaataaataa tcgaattatg atgcatgatt atttatcttt ccccataaac
    11101 ac
//
LOCUS       DACADF010000146        10905 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.148, whole genome shotgun sequence.
ACCESSION   DACADF010000146 DACADF010000000
VERSION     DACADF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.148"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            84..845
                     /locus_tag="ISB30_003862"
     CDS             84..845
                     /locus_tag="ISB30_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1991450.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            982..2319
                     /gene="adiC"
                     /locus_tag="ISB30_003863"
     CDS             982..2319
                     /gene="adiC"
                     /locus_tag="ISB30_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAP1991451.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2423..4066
                     /gene="eptA"
                     /locus_tag="ISB30_003864"
     CDS             2423..4066
                     /gene="eptA"
                     /locus_tag="ISB30_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAP1991452.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTQEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4063..4731
                     /gene="basR"
                     /locus_tag="ISB30_003865"
     CDS             4063..4731
                     /gene="basR"
                     /locus_tag="ISB30_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAP1991453.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4732..5832
                     /gene="pmrB"
                     /locus_tag="ISB30_003866"
     CDS             4732..5832
                     /gene="pmrB"
                     /locus_tag="ISB30_003866"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAP1991454.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(5808..5897)
                     /gene="pmrR"
                     /locus_tag="ISB30_003867"
     CDS             complement(5808..5897)
                     /gene="pmrR"
                     /locus_tag="ISB30_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAP1991455.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(6009..7511)
                     /gene="proP"
                     /locus_tag="ISB30_003868"
     CDS             complement(6009..7511)
                     /gene="proP"
                     /locus_tag="ISB30_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAP1991456.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7775..8653)
                     /locus_tag="ISB30_003869"
     CDS             complement(7775..8653)
                     /locus_tag="ISB30_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991457.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8650..10878)
                     /gene="crfC"
                     /locus_tag="ISB30_003870"
     CDS             complement(8650..10878)
                     /gene="crfC"
                     /locus_tag="ISB30_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAP1991458.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKIPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGIAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     NSESLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVREIFSRWLNQPD
                     WGRGNEKFPTETVDYSVLQRALSAEVDLYCQQMAKVMAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESMALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2737 a         2865 c         2657 g         2646 t
ORIGIN      
        1 catcatacaa ttatataacc atttcccggt atcgcttcgc tttagcgaga actggtcttt
       61 tattcgcact caggagtaca tgtatgagga tttgcagcga ccaaccttgt attgttttat
      121 tgactgaaaa agatgtctgg ataagggtga atgggaaaga acctattagc cttaaagcta
      181 accatatggc gttattaaat tgtgaaaata atattatcga cgtctcctct cttaataaca
      241 ctttggttgc tcatattagt cacgacatca tcaaagatta cctccggttt ctgaataaag
      301 atctctcgca aataccagta tggcaacgta gcgctacgcc catcctcacc cttccatgcc
      361 tgacgccaga cgtctttcgc gttgccgcgc aacatagcat gatgcccgct gaaactgagt
      421 cagaaaagga acgaacacgt gcattattat tcactgtgct atcccgtttt ctcgacagta
      481 aaaaattcct ttcactaatg atgtatatgt tacgtaattg tgtaagtgac agcgtttatc
      541 aaattattga aagcgatatc cacaaagact ggaatcttag tatggtagcc agttgtttat
      601 gtcttagccc aagtctgtta aagaaaaagc tgaaaagcga aaacaccagt tatagccaaa
      661 taatcaccac ctgccgcatg cgctatgccg taaatgaatt aatgatggac ggtaaaaata
      721 tctctcaggt atcacagtcc tgcggctaca acagtacgtc gtactttatt tctgtcttta
      781 aagacttcta cggtatgacg cctctgcatt atgttagtca gcacagagaa cgcactgtcg
      841 cctgattttt aaccttaacg aagagctata ttaataacgg catcagcgat aacccggtcg
      901 ataataattc aactatcgaa tgcaggcgta tgatatgacg taatttattg tcacgaagct
      961 cgccttcgca ggagtttaat tatgtcttcg gatgctgatg ctcacaaagt gggcttaatc
     1021 cccgtcaccc tgatggtgtc ggggaatatt atggggtcag gtgtttttct gttacctgca
     1081 aacctggcct ccactggcgg gattgccatt tatggatggt tggtgacgat tatcggtgcg
     1141 ctggggctct cgatggtata cgccaaaatg tcgttcctcg acccaagtcc tggtggttct
     1201 tacgcttacg cccgccgctg ctttgggccg tttctcggtt atcaaaccaa cgtcctctac
     1261 tggctggcct gctggatcgg caatatcgcc atggtggtca ttggcgtagg atatttaagt
     1321 tacttcttcc cgattctgaa agatccattg gtattaacca tcacctgcgt cgtggtgctg
     1381 tggatcttcg tcctgctgaa cattgtcggt ccgaaaatga tcacccgcgt acaggccgtt
     1441 gctaccgtgc tggcgctgat ccccatcgtc gggattgccg tatttggctg gttctggttc
     1501 cgtggtgaaa cctatatggc ggcatggaac gtcagcggcc tgggcacctt cggtgcgatt
     1561 caaagtaccc ttaacgttac gctgtggtcg ttcatcggtg tggaaagtgc ctccgttgcc
     1621 gcaggtgtgg tgaaaaaccc gaaacgcaac gtccctatcg ccaccattgg cggggtattg
     1681 attgctgccg tttgctatgt actttctacc accgcgatta tggggatgat ccctaatgcc
     1741 gcactgcgcg tttctgcttc gccattcggt gatgccgcac ggatggcgtt gggtgacacc
     1801 gccggggcca ttgtttcctt ctgcgcagct gcgggttgct taggttcact gggcggctgg
     1861 acgttgctgg caggtcaaac ggcgaaagcc gctgccgatg acggactgtt cccaccgatt
     1921 tttgcccgtg taaataaagc gggcacgcca gtggcggggc tgattatcgt cggtattttg
     1981 atgaccatct tccagctcag tagcatttcg ccaaacgcga ccaaagaatt cggtctggtt
     2041 tcttccgtgt cggtcatctt tacactggtg ccatatcttt acacctgtgc ggcgttactg
     2101 ctgctcggcc acggtcactt tggtaaagcg cgcccggcat acctggcagt tactaccatt
     2161 gccttcctct actgcatctg ggccgtagta gggtccggcg cgaaagaggt tatgtggtca
     2221 tttgtcactc tgatggtaat caccgccatg tatgcgctga attacaaccg gctgcataaa
     2281 aacccgtatc ccttagatgc accaataagc aaagattaat tccccttaat ccagcaaaca
     2341 taaaagccaa ccttaagaac ttaaggttgg cttaattttg ctttgcgagc atatgcgcac
     2401 tttgttcgat ggaaacaccg tgatgttgaa gcgcctacta aaaagaccct ctttgaattt
     2461 attctcctgg ttattacttg ctacttttta tatctcagtc tgcctgaata tcgccttttt
     2521 taaacaggtg ctacaggtgt taccgctgga ttcactgcat aacgtgctgg ttttcttgtc
     2581 gatgccggtc gtcgcattca gcgtgattaa tattgtcctg acactaggct ctttcttatg
     2641 gcttaatcgt ccactcgcct gcctgtttat tctggtcggt gcggctgcgc aatatttcat
     2701 catgacctat ggcatcgtca tcgaccgttc aatgatcacc aatatcattg atactacgcc
     2761 ggcggaaagt tatgccctga tgacgccacg aatgttatta acgctggggt taagtggcgt
     2821 attagccgcg attattgcct gctggatcaa aatcaaaccc accacctcac gcctgcgcag
     2881 cgttcttttc cgcggggcca acatcctgat ttctgtactg ctgattctgc tggttgccgc
     2941 actgttctac aaagactatg cctcgctgtt tcgcaacaac aaagaactgg tgaaatcctt
     3001 aagcccgtcc aacagcattg ttgctagctg gtcgtggtac tctcaccagc ggtcagccaa
     3061 cctgccactg atacgcattg gtgaagatgc tcaccgcaac ccattaatgc aaaatgggaa
     3121 acgtaaaaac ctgaccattt tgatcgtcgg agaaacgtcg cgcgcagaga atttctcgct
     3181 gaatggttat ccacgcgaaa ccaatcctcg tctggcaaaa gataacgtgg tctatttccc
     3241 taataccgcc tcttgcggca cggcaacggc agtttcagta ccatgcatgt tctcggatat
     3301 gccgcgtgag cactacaaag aagagctggc acagcaccag gaaggcgtgc tggatatcat
     3361 tcagcgagcg ggcatcaacg tgctgtggaa tgacaacgat ggcggctgta aaggcgtttg
     3421 cgatcgcgta cctcaccaga acgtcaccgc gctgaacctg cctggtcagt gcatcaacgg
     3481 cgaatgctat gatgaagtgc tgttccacgg gctggaagat tacatcaata acctgcaggg
     3541 tgatggcgtg attgtcttac acaccatcgg cagccacggc ccgacctatt acaaccgcta
     3601 tccgccgcag ttcaggaaat ttaccccaac ctgcgacact aacgagatcc agacctgtac
     3661 ccaagagcaa ctggtgaaca cttacgacaa cacgctggtt tacgtcgact atattgttga
     3721 taaagcgatt aatctgctga aagaacatca ggataaattt accaccagcc tggtttatct
     3781 ttctgaccac ggtgaatcgt taggtgaaaa tggcatctat ctgcacggtc tgccttatgc
     3841 catcgccccg gatagccaaa aacaggtgcc gatgctgctg tggctatcgg aggattatca
     3901 aaaacggtat caggttgacc agaactgcct gcaaaaacag gcgcaaacgc aacactattc
     3961 acaagacaat ttattctcaa ccttattggg cctgactggc gttgagacga agtattacca
     4021 ggctgcggat gatattctgc aaacttgcag gagagtgagt gaatgaaaat tctgattgtt
     4081 gaagacgata cgctgttatt gcagggattg attctggcgg cgcaaaccga aggctacgcg
     4141 tgcgatggcg tgagcaccgc gcggatggcg gaacaaagcc ttgaagccgg tcattacagc
     4201 ctggtggtac tggatttagg cttacccgat gaagatgggc tgcattttct tgcccgtatc
     4261 cggcagaaaa aatacactct gccggtgtta attctcaccg cccgcgatac ccttaccgac
     4321 aaaatcgccg ggctggatgt cggggccgac gattatctgg tgaagccttt tgcgctggaa
     4381 gagttgcacg cccgtatccg cgccctgcta cgacgccata ataatcaggg cgaaagtgag
     4441 ctgattgttg gcaacctgac gctgaatatg ggtcgccgtc aggtctggat gggcggtgaa
     4501 gagttgattc tgacgcccaa agaatatgct ctgctgtcgc gcctgatgct taaagcaggc
     4561 agcccggtgc atcgggaaat tctctacaac gatatctaca actgggacaa cgagccctcg
     4621 accaacaccc tggaagtgca tatccataac ctgcgcgaca aagtgggcaa agcccgtatc
     4681 cgcaccgtgc gcggatttgg ctatatgctg gtcgcaaatg aggaaaacta attgaatctg
     4741 atgcgttttc tgcgccgacc aatatcgctg cgccaacggc tgatattgac catcggggcc
     4801 attttgttgg tgtttgagct gatcagcgtc ttctggctat ggcatgaaag taccgagcag
     4861 attcagctgt ttgagcaggc gctacgcgac aatcgcaaca acgatcgcca catcatgcgt
     4921 gagatccgcg aggcggtcgc cagcctgatt gtccccggcg tctttatggt tagcctgacg
     4981 ctatttatct gctatcaggc ggtacgccgc atcacccgac cgctggcgga gctgcaaaaa
     5041 gagctggaag cgcgcaccgc cgacaactta acgcccattg ccattcatag cgccaccctc
     5101 gaaatcgatg cggtggtttc ggcgttaaac gatctggtca gtcgcctgac cagcacgctc
     5161 gataatgaaa ggttgtttac cgctgacgtc gcgcacgaac tgcgaacgcc actggcgggg
     5221 gtgcgtttgc atctggaact gctggcgaaa acgcatcaca ttgatgtagc accgttagtg
     5281 gcacgacttg atcagatgat ggagagcgtc tcccagttgc tgcaactggc gcgtgccgga
     5341 cagtcatttt cttccggtaa ttatcaacat gtaaaactgc tggaagatgt cattctcccc
     5401 tcgtatgacg aactcagtac catgctcgac cagcgccagc aaaccctgct attgccagag
     5461 agcgccgccg acatcaccgt tcagggtgat gcaaccctgc tgcggatgtt attgcgaaac
     5521 ctggtagaaa acgcccatcg ttatagcccg caaggcagca acattatgat taagctgcaa
     5581 gaagatggcg gagcggtcat ggccgttgaa gatgaaggac cgggtattga tgagagtaaa
     5641 tgcggggagt tgagtaaagc atttgtacga atggacagcc gttatggcgg aattggtctg
     5701 gggttaagta ttgtcagccg cattacacag ttgcatcacg ggcagttttt cctgcaaaac
     5761 cgacaagaga cttccggcac gcgggcctgg atacggctga aaaaagatca gtacgtggca
     5821 aaccagatat aaagaaagct gctgaccacc agcacgctga acacggtagt taaactttca
     5881 taaacacggt ttttcattgc actctcctcc gttaacctgg aggagagtat gcgcgtcaga
     5941 gattaagtga accttaagag ttcaattgac ctgataagac agcatcacat caggccatcc
     6001 gtttcagctt attcatcaat tcgcggatgt tgctgcacca ggcgggtacg tttcgcctgc
     6061 aaatcggcaa tctcgtggtc aatatcatcg attttctgct cgatattatc gtaatgctcg
     6121 acgagaattt ccttcgcttc ctgtatatct gacgccgccg gtgtcgcacc tttcaacgga
     6181 cgatttgccg tctctttcat ggttacgccg gtgattaaac caaccaccgc cactaccatc
     6241 aggtaatagg caggcatcat cagattctgc gagctttcga ccagccaggc cgccagtgtt
     6301 ggcgtcagac cggcaaccag caccgaaata ttaaatgccg ccgccagcgc gctgtatcgg
     6361 atatgcgtcg ggaacatcgc tggcaaggta gaagccataa cgcccgtaaa gcagttaagg
     6421 atcaccgcca gcatcagtaa cccggcaaaa atcaggccga tgacgttact gttaatcaga
     6481 ataaacgccg ggatcgccaa cacaaacagt gcaacactac caagtagcac aaacggacga
     6541 cggccaaaac ggtcactcag caagcccatc accggctgga caaacagcat accgatcata
     6601 atggcgataa taatcagcac cccgtggtct tcggagtaat gcaggttatg cgacaaataa
     6661 ctcggcatat aggtcagcaa catgtagtaa gtcacgttgg tggcaattac cagaccaata
     6721 catgtcaaca ggctgcgcca gtgtttggtg gcaatctctt taaacgagac tttcgggcca
     6781 tcctgcaaac cttcgcggtc gccctgttcc agtttatcga catgctgctg gaacgccgga
     6841 gtctcttcca gcgcatggcg caggtaaagc ccgataatcc ctaacggcag agcgataaag
     6901 aacggaatac gccagcccca atcgaggaag ttctcttcgc cgacaatggt cgaaattaac
     6961 accactacgc cagctcccag cacaaacccg gcaatagaac caaagtccag ccagctgccc
     7021 ataaagccac gtttacggtc aggggagtat tccgcaacaa atattgacgc cccggtatat
     7081 tcaccgccga ccgagaaacc ttgtgccatc ttacagatca acagcagaat cggtgcccaa
     7141 ataccaatcg tgtcgtagga cggtattaag ccaatacaga acgtactgat cgacataatc
     7201 acaatagtga tagcgaggat cttctggcga ccatatttat cgcccaacat accaaagaag
     7261 agcccgccaa gcggtcgaat cagaaaggga acggagaaag tggcgagtgc agcaaccatc
     7321 tgcacgctgg ggtcagcccc cgggaaaaaa actttaccta atgcgtaagc aacaaaacca
     7381 taaacaccaa aatcgaacca ttccattgca ttacccagtg atgctgcggt aatggcttta
     7441 cgcagtttac catcatcaat aatggtgaca tcacgaaggg taatcggttt tacttttttc
     7501 cttttcagca tagctttcct cgcagagttg gcccatttcc gcaaacgcca gaaaaatcag
     7561 cgcgacggcc gcaaaagggg agcgggaatt gcaggatgca atctctgtaa caagcgtagc
     7621 gggtttgctt acaccctccg gttaaggaat gtacaatcaa accagttgac gcctgaattg
     7681 ggcagttaat gaccttttta ccctaccagt gtttaaattt gtgatcaact tcacacatgc
     7741 atttcgttta ggactcattg gtgtaactga ttatttacac caaatttaca gagaagattt
     7801 cctcgaccat tgcccccgcc agacgctgcg ctgaacagag tcgtggcatc cccagtgcga
     7861 cagttcgcca gcgttgggtc actgaccagc aaacgggttg tttatctgcc gtagaccact
     7921 ttcccagcca acttaaaaaa tcagcatgat ccccgcccgt cgaaacggtc gaagaggcaa
     7981 accactgatg gtaatagtga cgcgtggtcg gggccgcatt gatactctct gccaaatgtt
     8041 gtgtcgcttt cacgcgcaaa tcacctagca acaacgccac cgcttgcggg cttcccagac
     8101 ggcaggcatc gccaaatgca ccccagcgca actcttcgag aacgataaaa cagcgcccag
     8161 gcaacgacca ggcgttatac gccccagcac gccagcgggt aaaaatttgc tcgctctgtt
     8221 cacccgcctg caccaatagg cctcgttgcg ttaacgcctt ttccttccag cgacaacggg
     8281 ctgaaaacca ctcatacaag gcgacaattt gctgggggag ctccgggaaa gcagagtgct
     8341 ggcggtgatc cattttttgc agcagcgcca gatgctgcgc gatctgcgtc agcgccagat
     8401 gaccgggatc cagcatcgac gccagttttt ccaccaatgt cagacgcagg ctaagcggcg
     8461 catttaacat gccgccgttc gtccatgtct gaagctgcac gcgcgcaaga agttcctgca
     8521 tcagtcgttc acgaaactgc tgctgatggg cttgtggaag gcgggcatca tcgccctgcg
     8581 ccagatccac taaaaagcgc ggatgaaccg actccagcac gcgaccgggg ccgtccagta
     8641 acgtcttggt caatatcgtt ctgccgtgaa aagtgtttga atatcatcgc gtaacagctg
     8701 agcgtcggtg taaatccagc cgtgagtcat cacagtctgc tgcaattgct gttgcatcag
     8761 cctgaccacc gattcatttt gttgacgcag agccatactt tcgcgtaaac gcgtctgtaa
     8821 ttccgtcaaa catgaagcga actcagcgaa aaaagtattc atgcctgccg taacagattc
     8881 atcgacctgc tctgccataa ctttagccat ttgttggcaa taaagatcga cttctgcgct
     8941 taatgctcgt tgcaacacac tgtaatcaac cgtctctgtc gggaatttct catttccccg
     9001 tccccagtcg ggctgattca accagcgcga aaaaatctca cgcacaacgc ctaaacgcgt
     9061 gctctgctcg tccgttgctt cctggcgcga aatgacttca ctgaacagct ggcgggtgtt
     9121 gaagtgggga actacgccgt gaaaaacagg aaaatgaaac ccaggacgaa acccagactc
     9181 actcaattcc attttgactt gttgctcaat ggggcgaata acatcggtta acacccggca
     9241 aagggtggac tcaagctcgg caaagcgcag cgtaaagtcg cgactgatgg tgttctgcgc
     9301 cgtctgtaac agtgtctcac agcgggtacg catctcactt aacgactccg aattatcctg
     9361 aaacaacgcg gctaactgcg cattcaccgc atcttgctgt tgacgcagaa agtggttggc
     9421 ggaggtcagg gccaactcga tttcatgttt aatctcgccg ctcacctgcg cctgattgag
     9481 ttgcaatagc tgcaaacttt cttcgacctg atggatattt tgccgcaatt gttcacaagc
     9541 gacgtttaac ccgtgcgcac gaaaatccag gtattcccgc gcctgctgcg cgtaattcaa
     9601 cagtttatgc gcggcagatc gcagagcata caacgaggcg ttagcgtaag cggcatgaag
     9661 caacgcctga attggctggg cgaacagcga atcttcccac aactgatcgg cagcatggcg
     9721 aatatgttcg aggtccgcca gatcggcatg acgccagcgc ctgccgagcg cggcatgggc
     9781 aaaatcttcc acccagcgtt gttgctctgg cgcgggtaac ttaccgttgt tggctaactc
     9841 atggcgcgcc cgattcgcca ggtagcccca catcgacgac accggaaata tctgctgtgg
     9901 cgtaatacag cctttcatca gcgttccgga aatcagtgcc cgcacctggt cggcgtcgtc
     9961 actgttacga tcctgttgat cgaacttatt gaccagcaca tacaacggca ccgattgccc
    10021 caccgccaaa attgcctcac ggacctcttc atcggagatc gatttcagtt gcgtataatc
    10081 cagcaccgcc agtaccgccg aggcgcgtgc cagctgctgg ttaagcattt tttgcagatg
    10141 cggttgcccg gcttcatttg gcccgggggt atccagtaac gtcaactgac cgggataact
    10201 ctccagcccc gccagatgga caaactccac ttcaatcacg ggaatatgct caatggcggc
    10261 gtaagcagaa aaaggaaaat cgacgtccag cgccttcgcc agtcgcacca aatcattcaa
    10321 acttttcaga caatgaaaaa taggttgagc acccagataa tatttttcga aagcgatgcc
    10381 attttcgatc cgctgcataa gcgcacgcat atctttatct atttccagca catcggtcag
    10441 atgcttaata tcgcaatcac gcaggcgctg ttgtaattgt tgaattaaat ggtcgattgg
    10501 cgcgacatgt gaaaaatgca gtaccggttc tttttgcccg ggcgtatggc gaataagcgt
    10561 tggcagcgca gtcattgggc gattgcgatt aggcagaacc tccgtaccaa caatggcatt
    10621 aatggtggtt gatttccctg ctttcatggt accgacaatc gccagcacca tttccagccg
    10681 ggaaatttta cgcaactcat tattcagcat cgcgtgacgt tcggcgatat taggctgact
    10741 ccagggtaaa gccagttgtg gcgcatcgtc tccgggtaca gagagaggta ttttttccag
    10801 taactgcaac tgttgtcggg aaagctgtaa caggcgttca gcctcctgac ttaactcata
    10861 cagggtctgt gtgtacatag aatattcttc cttaaagcaa atttt
//
LOCUS       DACADF010000147        10864 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.149, whole genome shotgun sequence.
ACCESSION   DACADF010000147 DACADF010000000
VERSION     DACADF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.149"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            2..961
                     /locus_tag="ISB30_003871"
     CDS             2..961
                     /locus_tag="ISB30_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAP1991459.1"
                     /translation="MGFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRVKQLARRKTI
                     TPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHIAKKLLPYIP
                     EQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLVARVKVALEY
                     NRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCVGNSSERVPW
                     LCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEPEEDADDLSV
                     GYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            1113..2341
                     /locus_tag="ISB30_003872"
     CDS             join(1113..1428,1428..2341)
                     /locus_tag="ISB30_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1991460.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVIPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSFVSRCLRVSRAQLHVILRRADDWKDGRRSRHTDDTDV
                     LRRIHHVIGELPTYGYRRVWALLRRQTELDGMPAINAKRVYRTMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFD
                     SETVQDVMLGAVERRFGSELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQWASDGLSDNRCLEI"
     gene            2841..4931
                     /gene="iha"
                     /locus_tag="ISB30_003873"
     CDS             2841..4931
                     /gene="iha"
                     /locus_tag="ISB30_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HAP1991461.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASAQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTVGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(5149..5308)
                     /locus_tag="ISB30_003874"
                     /pseudo
     CDS             complement(5149..5308)
                     /locus_tag="ISB30_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            5763..6035
                     /locus_tag="ISB30_003875"
     CDS             5763..6035
                     /locus_tag="ISB30_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991462.1"
                     /translation="MQNNKKQGHIMKDEIHSTSINPDVVEQFLALMNTHCKSSEEALA
                     MLSTLCYNLMVTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            6326..6685
                     /locus_tag="ISB30_003876"
     CDS             6326..6685
                     /locus_tag="ISB30_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991463.1"
                     /translation="MKDNSTTATPEAWKMRFLANGTSGTWFYTDDITVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHEV"
     gene            6689..6904
                     /locus_tag="ISB30_003877"
     CDS             6689..6904
                     /locus_tag="ISB30_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991464.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALHAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(7482..7804)
                     /locus_tag="ISB30_003878"
                     /pseudo
     CDS             complement(7482..7804)
                     /locus_tag="ISB30_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            7824..8423
                     /locus_tag="ISB30_003879"
                     /pseudo
     CDS             7824..8423
                     /locus_tag="ISB30_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544014.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            complement(8367..9899)
                     /locus_tag="ISB30_003880"
                     /pseudo
     CDS             complement(8367..9899)
                     /locus_tag="ISB30_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(10370..10804)
                     /locus_tag="ISB30_003881"
     CDS             complement(10370..10804)
                     /locus_tag="ISB30_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991465.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT         2797 a         2602 c         2724 g         2741 t
ORIGIN      
        1 aataggtttc tggtatgtga tagccctcgc aggacagtct aatggtatgg cctacggtga
       61 aggtattccc ctgccagata cactcgacaa acccgagtcc cgtgtaaaac aactggctcg
      121 tcgaaaaaca atcacaccag gaggaaaaga gtgtaaattc aatgagatta ttccggcaga
      181 tcactgcctg catgatgttc aggatatgag tggatatcac catccggctg ctgatttgca
      241 taaaggggaa tatggttgtg tcggacaggg attgcatata gctaaaaagt tactgccata
      301 catacctgag caagcaggga tattactggt tccttgttgc agaggaggtg ccgcctttac
      361 tgttggagct gaaggtatgt atgtacctga tacaggggca acagctgatg caatgcggtg
      421 gggcaccgga acagcactct atgaggactt agtagcccgg gttaaggtag ctctggaata
      481 taacagaaag aataaactgc tatctgtctg ctggatgcag ggggagtttg atcttatgtc
      541 accagattat gaaaagcatc ctgacttgtt ttatcagatg gtaacttctt tcaggtctga
      601 gttatctgaa tactcatctc agtgtgttgg taattcgtca gaaagagttc catggttgtg
      661 tggtgataca acctggtact ggaaggaatc ttatcaaaaa gaatatgatt ttatatatgg
      721 acattacaga caacggactg atgatgaaat acattttctc agctttcagg atagtaaccg
      781 acatgaatta actaatgagc cagaagaaga tgccgatgat ttatctgtcg ggtatctggg
      841 gtcatcatgg aggacagaat taagctggac aacgtcacag agaagtacac attttaattc
      901 aatggccaga aggggagtta ttgctgaatg ttatgcgcaa aaaataagaa attatttgta
      961 gacaagttca ggatatatct acagctctga catgattctc tgagagacat gacggagtgg
     1021 ttagactggc cccctgaatc tccagacaac caatatcact taaataagtg atagtcttaa
     1081 tactagtttt tagactagtc attggagaac agatgattga tgtcttaggg ccggagaaac
     1141 gcagacggcg tactacacag gaaaagatcg ctatcgttca gcagagcttt gaaccgggaa
     1201 tgacggtctc ccttgttgcc cggcaacatg gtgtggcagc cagccagcta tttctctggc
     1261 gtaagcaata ccaggaggga agtcttactg ctgtggctgc cggagaacag gtcattcctg
     1321 cctctgaact tgctgccgcc atgaagcaga ttaaagaact ccagcgtctg ctcggcaaaa
     1381 aaacgatgga aaatgaactc cttaaagaag ccgttgaata tgggcgagca aaaaagtgga
     1441 tagcgcacgc gcccttattg cccggggatg gggagtaagc ttcgtcagcc gttgtctccg
     1501 ggtgtcgcgt gcgcagttgc acgtcattct cagacgagcc gatgactgga aggacggccg
     1561 ccgcagccgt cacacggatg atacggatgt gcttcgccgt atacaccatg ttatcggaga
     1621 gctacccaca tatggttatc gtcgggtatg ggcgctgctt cgcagacaaa cagaacttga
     1681 tggtatgcct gcgatcaatg ccaaacgtgt ttaccggacc atgcgccaga atgcgctgtt
     1741 gcttgagcga aaacccgctg taccgccatc gaaacgggca cataccggca gagtggctgt
     1801 gaaagaaagt aatcagcgat ggtgctctga cgggtttgag ttccgctgtg ataacggaga
     1861 aaaactgcgg gtcacgttcg cgctggactg ctgtgaccgt gaggcactgc actgggcggt
     1921 cacaacgggt ggcttcgaca gtgaaacagt acaggacgtc atgctgggag cagtggaacg
     1981 ccgctttggc agcgagcttc cggcgtctcc agtggagtgg ctgacggata atggttcatg
     2041 ctaccgggcg aatgaaacac gtcagttcgc caggatgttg ggacttgaac cgaagaacac
     2101 ggcagtgcgg agtccggaga gtaacggaat agcagagagc ttcgtgaaaa cgataaagcg
     2161 tgactacata agtatcatgc ccaaaccaga cgggttaacg gcagcaaaga accttgcaga
     2221 ggcgttcgag cattataacg aatggcatcc gcatagtgcg ctgggttatc gctcgccacg
     2281 ggaatatctg cggcagtggg ccagtgatgg gttaagtgat aacaggtgtc tggaaatata
     2341 ggggcaaatc cattgtcagc gccaacaatg tgggctgaca tgataaagct gtctaggaaa
     2401 tcgttcgcct cctcagcgga caatccaaat ggtgattgtc tctgttaaac gtttattttg
     2461 aaggtcgact gaataaggtg atgacgctgt agaatttttc acgtgccaca gaattttgaa
     2521 cgctttctct tacaatattt caatgtttct atcagtattc gccggaagaa gtcatcgacc
     2581 aaatcatccc agtcgtctcg caccactgac cattcatggt tgacatgtgg tggaaatccg
     2641 cttctacagt aaccattttt tattcgcaaa accgaacacg ccatacggat agctgttaac
     2701 tggcatgccg aggcagtcgt tatttatatt tagttttgtc aataatcttt attttttgta
     2761 aaaggcaaat ataaattatt ctcattattg tttgtattta tgtattgtct tgccggttaa
     2821 catgatcgga gattagtaat atgcgaataa ccactctggc ttccgtagtc attccctgtc
     2881 tcggattttc agccagcagc atagctgctg cagaggatgt gatgattgtc tctgcatccg
     2941 gctatgagaa aaagctgact aacgcagccg ccagtgtttc tgtgattagc caggaggaat
     3001 tgcagtccag ccagtaccac gatctggcgg aggctctgag atcagtagag ggtgtggatg
     3061 ttgaaagtgg tacgggtaaa accggtgggc tggaaatcag catccgagga atgccagcca
     3121 gttacacgct gatactgatt gatggtgttc gtcagggcgg aagcagtgac gtgactccca
     3181 acggtttttc tgccatgaat accgggttca tgccccctct ggccgccatt gagcgtattg
     3241 aggttatcag ggggccgatg tccacactgt atggctctga tgcgatgggc ggtgtggtga
     3301 atatcattac cagaaagaat gcagacaaat ggctctcttc cgtcaatgca gggctgaatc
     3361 tgcaggaaag caacaaatgg ggtaacagca gccagtttaa tttctggagc agtggtcccc
     3421 ttgtggatga ttctgtcagc ctgcaggtac gcggtagcac acaacagcgt cagggctcat
     3481 cggtcacatc actgagcgat acagcagcca cgcgtattcc ttatcccacg gagtcacaga
     3541 attataatct tggtgcacgt cttgactgga aggcgtcggc gcaggatgtg ctctggtttg
     3601 atatggatac cacccggcag cgttatgata accgggatgg gcaactgggg agtctgacgg
     3661 ggggatatga ccggaccctg cgctatgagc gaaacaaaat ttcagctggc tatgatcata
     3721 ctttcacctt cggaacatgg aaatcgtatc tgaactggaa cgagacagaa aataaaggtc
     3781 gtgagctcgt acgcagtgta ctgaagcgcg acaaatgggg gcttgccggt cagccgcggg
     3841 agcttaagga atcgaacctt atcctgaatt cattactgct tacccctctg ggagaatctc
     3901 atctggttac ggtggggggc gagtttcaga gctcgtccat gaaagacgga gttgtccttg
     3961 ccagcacagg tgaaactttc cggcagaaaa gctggtcggt atttgctgag gatgagtggc
     4021 atctcacgga tgcacttgcg ctgactgtgg gcagccgcta tgaacatcat gagcaattcg
     4081 ggggacactt cagtccgcgt gcatatctgg tctgggatgt ggcagatgcc tggacgctga
     4141 aaggcggtgt gaccacggga tataaggcac ccagaatggg gcagctacat aaagggatta
     4201 gtggtgtatc cgggcaggga aaaacaaatc tacttggtaa ccccgacctg aagccggaag
     4261 agagcgtcag ttatgaggct ggggtgtatt acgataaccc cgccggtctg aatgccaatg
     4321 tcacaggttt tatgactgac ttctccaaca agattgtctc ttattccata aatgataaca
     4381 ccaatagcta tgtaaacagc ggaaaggccc ggttgcatgg tgtggaattt gccggcacat
     4441 tgccgctgtg gtcagaggat gtcacgctgt cactgaatta cacctggacc cgaagtgaac
     4501 aacgtgatgg tgataacaaa ggtgcgccgc tgagttatac ccctgaacac atggtgaatg
     4561 cgaaactgaa ctggcagatc accgaagagg tggcatcatg gctgggtgcc cgttatcgcg
     4621 ggaaaacacc acgtttcacc cagaattatt cgtcactgag cgctgtacag aagaaagtgt
     4681 atgatgagaa aggagaatac ctgaaagcct ggacggtggt ggatgcaggt ctgtcgtgga
     4741 agatgacgga tgccctgacg ctgaatgctg cggtgaataa cctgctcaac aaggattaca
     4801 gtgacgtgag cctgtacagt gccggtaaga gtacgctgta tgccggtgat tacttccaga
     4861 cgggatcatc aacaacagga tatgtgatac ctgagcgaaa ttactggatg tcgctgaact
     4921 atcagttctg ataataacaa aacgctatca ctgacggtag aatacgttgc cactgtaact
     4981 cctggcggaa cagtggcaac gtattaggtt aagtgcattt ccgatccgct aatgagattt
     5041 cgttaccaac aactaatatc gtcacaggaa atgcacggat tatttttaac ttatcattta
     5101 catacttgtc cagagtgtaa gcgcaccgtg aaggacgtgg ggtaaaaatt agtgtacaga
     5161 gagagtgacg ttccagggca acaactcttt catgcggttg gcaggccagg tgttgattac
     5221 actgatcacg tggcgtagcc acgtttccgg ctcgattccg ttaagttttg cagctaccga
     5281 tcaggctgta catcactgcc gcactatcgc tcgtcatctc aaagtccggt ctcgtcagca
     5341 ggaaggtatc attctctccc gccatttttc cgggggcccg gtcagataag tccctttgtc
     5401 tatcgctgac tcctggctca taacccggtt agcagaatgc agggtcacca cctcgccacg
     5461 accgaatccg gataagtcaa ttgcaccttc tcaatcgcca ttttgtcaca attaagtttg
     5521 ataaatcatg tcacctctgc actgaatctg ctccagtgcc tcattggtct ccgtccaccc
     5581 ctttaaactg ccggataaga ctgctgcaca gcgccttccc cggaatcaaa tcactttccc
     5641 tgtccgccac aatccgcttc atgccacctc gtattttgtt tcagagaccg gaatctgcca
     5701 gaaagtgact acgcgtgcac atatcggttt tctgggttac atgcatcgtg ttattttctg
     5761 aaatgcaaaa taataaaaaa caaggacaca taatgaagga tgaaatacac agtaccagca
     5821 tcaacccgga tgttgttgag cagtttctgg cccttatgaa cacacattgt aaatcctcag
     5881 aagaggcgct cgccatgctg agtacgcttt gttacaacct gatggtgaca tgcggtatcc
     5941 attccatgga cagccagatg ctcaatgggc atgggatatc catgcaactt caggggattg
     6001 atattgccga aaggccaggt aagaatattc actaacctga acagggtctg aaaacgctgt
     6061 cttttcaggc ctctgcaggc aaaaactgat gacaacaaaa tttctgcgtt tgtaccccac
     6121 cgatgaaaaa gacaaaaact cacaccgggc ttctagccag taaagataaa acccgacgtg
     6181 tcagcctcta cgaaacgcca acggcatggt gtatcagggg gcaggagtgc tacagcaaaa
     6241 gtacaggacg ccgctgtggc agccacgaca gtctgagcag actgcgcctg gacagcatta
     6301 agcctgttga atgagtggtg gcaccatgaa agataacagc acaaccgcca ccccagaagc
     6361 ctggaaaatg cgttttctgg ccaacgggac atccggcaca tggttttaca ctgacgacat
     6421 tacagtcact gacgcgtggc ttcgcgcgca cccggatgat gtggaggtta tccctctctg
     6481 ccggatacca ccccgtcagc attcccatga acaacatatt gaagaactcc gtgcagccct
     6541 ggcccggatg ctgcatatgc atcatatgat gctggaaacc acgcattaca gggaaaacat
     6601 caccgatgcc cgttgtatcc gtgaaatgca tgaagctcca ttacaggcgc tcaatattct
     6661 ggccagatac ccgcacgagg tgtgacgcat gtggctactg attttctggc tttcatctcc
     6721 ctttaatgtc tcttccggtg atgcccggca gcccacactg gcggtgcaca gccaggcatt
     6781 tcataccgaa cgggcctgtc gcgatgcatt gcacgccatt aaaaaggaga atgttggcga
     6841 attttctctg cggggcgttt gtgtttctga cggaaatacg aaggaggaag ctgagagctc
     6901 atgacggata ttactgacag acatacactt cgccgtatgt cctggtcgga actcttcacg
     6961 gcagcacagg aagcggaatt tcagcgtgat tacgagcgtg ccagaatagt gtggtcattc
     7021 gccttacatg ttgcaacaac cacgattaac aaaaatctgg ccatagctca tattcgacgc
     7081 tgcaatactt tgttgcataa atcaaaaaca gtacccggaa acaacacggg gggccggtca
     7141 gtgtgcctga gaccccaaca tccccggaga tgactgacat tccggactga cgcagttgct
     7201 gcatatctgt ggcaatacgt tgtatccagc cagacaggtt atcccgcgta gtgacaatta
     7261 acccgctttc ctgcaccagc ttcagccggg ccgtcgggga ttttaccagc cggaaatatc
     7321 agtaaaacaa catatcatta tgccatggtg tacgcggcca gtgtacgctt acttaaggaa
     7381 tgagggataa attggatatt attacttcac tttgcttttt tatgagattc atcacataag
     7441 tggggtttta ctgttaaata atctgaattt atttgatatc cgtaaagcag atgtctcagt
     7501 tcgctgtttc caacttttgt tattcctctg gggcgaatgc tgtttccgct ggaatattca
     7561 cctggcacca gtcccagaaa agccatgagc tgttcaggat tggaaaatcg cgaaaaatca
     7621 cccagttcag caaccagggt tattgcgatt agttgaccaa cacctttaag agcctgcaac
     7681 tggcagacca gattgcacag agatcactca ggtaacaggc gagatatttc ctgatcgagt
     7741 tgaagtatgc gatcttcaat ctgctccaga ttctgacaat aatgttgaaa gcaatctgtt
     7801 gagatagcag accaaatgat tgagcgagtg ttagtgatga caaacgtatc caggtacttc
     7861 ttatctcatg gtgttttggt ggatttcttg aggcggcagc cggatacggt acagcggtag
     7921 caatccccat tggtatactg attgctctgg gctttaaccc actaaaagca gcgattgcat
     7981 cactggttgc taatacagtc cctacagcgt ttggggcagt aggtatacct gtctctgtac
     8041 ttgctgaaca agttaattta cctgtaacca cactcagcgg gacaattatt cttcaactgg
     8101 cactgttcaa cattctgctt cctttcgtca tcgtcgcgat tatcggtggt ggtatgaaga
     8161 ctatacgagg cgttggacta attaccctga tgtgtggtgt cagtacatta attccccagt
     8221 atctggtggc tgttaatctt ggcgcggaac tcccggcttt cgcgggtagt cttgtcagtc
     8281 tgatagtaat agtctttatg gcgaaaaacc gtaaagaaaa aaccgcccct gaattgggga
     8341 tgcggacgat ttcctagact gctttattat gccagcccac attgttggcg gtacatttca
     8401 gggctgactg cacccagcga taattttata cgccgctcgt tataccatct gatgtaagca
     8461 tctacatgct gcatgagctt ttctggcgtg atgcccgacc atttacgccc ataatacatt
     8521 tccgttttca gtctgccgaa aaaaccttcg catgcggcat tatcaggtga acatccttta
     8581 cgggacattg agcgaataag acctgctgca ttcactcttt ccagccagcc tggccagcga
     8641 taatgcccac ctctgtcact gtgtatcacc ggtcgttcgc cagcatttaa cgtttcgaca
     8701 gcgctatcca gcatagtgtt gaccagctca gcatcggggc gtgtactgag agaccagcta
     8761 ataacttttc catcgaagca gtccaccacc ggtgacagcc agactttacc tgcagggagt
     8821 tggaactccg tgatatctgt cagccatttc tgattaggtt gctacgcctt aaaatctctg
     8881 gcgataaggt tatccggagc cggtccgatt tctccgcaat atgagctgta gcgacgacga
     8941 cggttacggc tgacgacaag ctgttcctcc accataagtc tgcgtacaac cttttctgat
     9001 agccgccccc cctcatgcct gagcatcgca tgcaggcgac gatagccata acactggtaa
     9061 ttactgttaa atatatctgt cagcatcgta cgtatcgtgg cgtatttatc accggctttc
     9121 agtgcagccc ggtgataaaa ataactgcta cgggtaaggc ccagaacatg aagtaattct
     9181 gtcaggggat atgtttgcct cagggcatca gcgatctttg ttttttctct gttgttcagg
     9241 tgactgatac tgatgcccgg gtcttttttt atgatttcct ccgccttttt cagaatatcc
     9301 agctccatct gccggcggcg tatttcctga ttcagtcggg cgacttcctc ccgcaacgca
     9361 tcgcgttctg cctccaggga aggttcgtta tgtttacgca tagtctggta agcactgttg
     9421 ccgataattt catctttcta tttatacaat accgcacgac tgacaccaat acgcctggcg
     9481 atctcactgg ctgatacttg tcgtgtacag agtgccatga cagcctgacg tttgacttct
     9541 ggttcaaagg gcgcaaccgg gttggcctta ctggtaaata tacgtcgtct gtcgggataa
     9601 agttcattaa cccagcgagc gagtacatca gtgcagggat aacccagggc tctgctggta
     9661 aacgccaggc agcagccgtg gttaagataa tgttcaacgg caacctgttt ttgctcatcg
     9721 gagtaacggt gcttatgacg aatggattct tttgcgccac cacctgcttc ccatgagcgg
     9781 atccaacgcc gcagatttct tttcgaaggg tagcccagtt cgcgtacgac gggagccagc
     9841 ttcttaccat atttgaaata aagctcaatg gcgcggattt tttcttcttc agtaaacatg
     9901 ttttatctcc tgagagtcca ggaaatcgtc cgcatccccc aacgagctgc tgtttaaatt
     9961 cgggaggata attagggcag ccttttcgcc tgccgggagt cacatttttc tgcatatctg
    10021 acactttggt tcccactact tatttggtgg acaccacttt gtctaattcg tcagattctg
    10081 accagacggt tcaggctgta cgcttaccac tattttatcc ctgttgaatt tggttaagcc
    10141 ggtgcagacg cgccgggaaa cgcacacctg aaccggtaca ataaaataca atacaacccc
    10201 ctgttactgg agatacgaat ggaatgacga ggcagcctgt cagtcacagg gggctgttgg
    10261 cataaaatca atggaaaggc caccaacacc ggaattaagc tccacagtcc ccgcactggc
    10321 aatccattcc cgggaaaggc atcaccgcaa actgctccgg aggcaaccat tacagcctct
    10381 gtaacggatt atcagccctg gcatcttccg acggcacctc ctgccgcccc cacgccctgc
    10441 tgtatacccc ggtaccgcag cgtgggcagc acttttcccc ctcataatct tcgtcacaca
    10501 tcacacaaaa ccactttctc accttcaccg tataatccgg aaccgtcatg ccctgcagat
    10561 gcaaacgacg ggcctctttc tctgccgggt caagccaggt acatcgcagc aggatttttt
    10621 cagcgacagt catctggtca tgttcaaacc acggtggttg tccgtcattg ccccgctgaa
    10681 gtgttaacgg gcatccacag gacacacagc acaaaacgtc ctgtcggttg tgcttcgcag
    10741 cttctttcgc cgtaacaaaa tggcctttgc tgtcattcgc catataacag ttcagaattc
    10801 gcatatttcc tcctgagttg tccggggaat actgaatcct ccggtctggc agtgtgtgca
    10861 gtac
//
LOCUS       DACADF010000148        10762 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.150, whole genome shotgun sequence.
ACCESSION   DACADF010000148 DACADF010000000
VERSION     DACADF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.150"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            25..813
                     /locus_tag="ISB30_003882"
     CDS             25..813
                     /locus_tag="ISB30_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP1991466.1"
                     /translation="MVRKGCNSLVRAEKILTHIAWVGMASYMELLNKFQYPKSSLLNL
                     LNVMVECGFLIKNKNGYYSLGIKNYELGCQALHRQNIFEVTKRPMQELSLKSGLVCHL
                     GAMESISAIYLDKIESPDSVPTSKSWIGKKIELHITALGKALLAWKTREELDYFLEAL
                     TLTPHTRNTFTDKKLFLEELQKTRLRGWAIDNEESTYGAVCLSMPVFNMYNRVNYAIS
                     LSGDPVVYSGNKIDSYLELLRKCAEQISYGLGYRNENEHLRKGN"
     gene            818..1723
                     /locus_tag="ISB30_003883"
     CDS             818..1723
                     /locus_tag="ISB30_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HAP1991467.1"
                     /translation="MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYL
                     GTGGEFSQMNTAQRMALAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGI
                     VAINPYYWKVAPRNLDDYYQQIARSVTLPVILYNFPDLTGQDLTPETVTRLALQNENI
                     VGIKDTIDSVGHLRTMINTVKSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPE
                     LSVGIYRAWREGDLATAATLNKKLLQLPAIYALETPFVSLIKYSMQCVGLPVETYCLP
                     PILEASEEAKDKVHVLLTAQGILPV"
     gene            1734..3701
                     /locus_tag="ISB30_003884"
     CDS             1734..3701
                     /locus_tag="ISB30_003884"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HAP1991468.1"
                     /translation="MSVRNIFADESHDIYTVRTHADGPDGELPLTAEMLINRPSGDLF
                     GMTMNAGMGWSPDELDRDGILLLSTLGGLRGADGKPVALALHQGHYELDIQIKAAAEV
                     IKANHALPYAVYVSDPCDGRTQGTTGMFDSLPYRNDASMVMRRLIRSLPDAKAVIGVA
                     SCDKGLPATMMALAAQHNIATVLVPGGATLPAKDGEDNGKVQTIGARFANGELSLQDA
                     RRAGCKACASSGGGCQFLGTAGTSQVVAEGLGLAIPHSALAPSGEPVWREIARASARA
                     ALNLSQKGITTREILTDKAIENAMTVHAAFGGSTNLLLHIPAIAHQAGCHIPTVDDWI
                     RINKRVPRLVSVLPNGPVYHPTVNAFMAGGVPEVMLHLRSLGLLHEDVMTVTGSTLKE
                     NLDWWEHSERRQRFKQLLLDQEQINADEVIMSPQQAKARGLTSTITFPVGNIAPEGSV
                     IKSTAIDPSMIDEQGIYYHKGVAKVYLSEKSAIYDIKHDKIKAGDILVIIGVGPSGTG
                     MEETYQVTSALKHLSYGKHVSLITDARFSGVSTGACIGHVGPEALAGGPIGKLRTGDV
                     IEIKIDCRELHGEVNFLGTRSDEQLPSQEEATAILNARPSHQDLLPDPELPDDTQLWA
                     MLQAVSGGTWTGCIYDVNKIGAALRDFMNKN"
     gene            3808..5157
                     /locus_tag="ISB30_003885"
     CDS             3808..5157
                     /locus_tag="ISB30_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HAP1991469.1"
                     /translation="MPLIIVVAGIALLLLLTIKIKLNTFVSLIIVSIAVAIASGMDLS
                     KVVTSVESGLGGTLGHIGLIFGFGVMLGRLLADAGGAQRIALTMLNYFGKNKLDWAVV
                     CSAFIVGIALFFEVGLILLVPILFAIAREAKISPMFMCVPMLSGLLVAHGFLPPHPGP
                     TVIAREYGADVGLVLIYGIIVGIPTFILCGPVLNKFCQRIIPDAFKKEGNIASLGATR
                     RFSESEMPGFGISFLTAMLPVILMAVVTIIQMTHAKSAADSGLFYNVILFLGNSTIAM
                     LISLLFAIYTMGLGRGKTIPDLMDSCGKAIAGIAGLLLIIGGGGAFKQVLIDSGVGQY
                     ISTLVSGMDINPILMAWGVAAFLRICLGSATVAAISTAGLVIPLLAVHPNTNLALITL
                     ATGAGSCICSHVNDASFWMIKDFFGLTTKETLLSWTLMSTLLSISGLIFILLASLVL"
     gene            5504..6490
                     /locus_tag="ISB30_003886"
     CDS             5504..6490
                     /locus_tag="ISB30_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1991470.1"
                     /translation="MDRDQTNSSLFNDDPVLHATWLYYQEGKSQTEVAAIMGVSRVTV
                     VKYLQTARENGLVHINLDVNVFGSIDAALQIRDKFNLQRVIIVPDGEHAGKRDDTKLM
                     RTRLSRAGGMYLNQVIENGDVLGVAWGRTIHQMSKTMTPKSCKNVTVIQMLGSMPSQP
                     DLTIIESSSQIAYKLSGRVASLHVPAVVSSARLAMELQAEPIIRSNFDVLTRCTKAFF
                     VVGNALDENPLIRVGVLNKKEMQTYRDLGAVGVICGRFYDKEGMPVVADVDQRILGIS
                     LAQLRQIERKIFLAGGERGYDATLGALLGGYVTDLIVDEGTAEFLLACELPH"
     gene            complement(6531..7535)
                     /locus_tag="ISB30_003887"
     CDS             complement(6531..7535)
                     /locus_tag="ISB30_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HAP1991471.1"
                     /translation="MFIVESYAVAIIMCFITMICWGSWANTTKLVSNKKWEFPLFYWD
                     YSIGLLLCSLLFAFTLGSMGEAGRSFIPDIQQASSSSLMSAILAGIIFNISNILLVAS
                     INLAGMAVAFPVGVGLALALGVITTYIGNPQGDPLILFLGVACVVSAIIFTAIAYGRV
                     TQEADKSRRNKGLITAILAGIIMGWFFRFLADSMSDNFSQPASGLMTPYSALVLFAVG
                     LFLSNFVLNTLVMKKPISGEPVNGKMYFSGSLRDHVCGWLGGMIWCVGLAFSLIASGQ
                     AGYAISYGLGQGATMIAVIWGVFIWREFASAPAGTNKLLLTMFISYIVGIVLIIAANQ
                     "
     gene            complement(7639..8280)
                     /locus_tag="ISB30_003888"
     CDS             complement(7639..8280)
                     /locus_tag="ISB30_003888"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAP1991472.1"
                     /translation="MERIKLAEKIISTCREMNASGLNQGTSGNVSARYTGGMLITPSG
                     IAYSKMTPDMIVFVDDKGIPEAGKIPSSEWLFHLACYKARPELNAVIHTHAVNSTAVA
                     IHNHSIPAIHYMVAVSGTDHIPCIPYYTFGSPELADGVSKGIRESKSLLMQHHGMLAM
                     DVTLEKTLWLAGETETLADLYIKCGGLHHDVPVLSEAEMTIVLEKFKTYGLKA"
     gene            complement(8311..9426)
                     /gene="mtnA"
                     /locus_tag="ISB30_003889"
     CDS             complement(8311..9426)
                     /gene="mtnA"
                     /locus_tag="ISB30_003889"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HAP1991473.1"
                     /translation="MNIKGKHYRTVWVSGDGKAVEIIDQTKLPFKFEVVALTSAEMAA
                     TAIQEMWVRGAPLIGVVAAYGIALGMNHDASDMGLQRYYDLLIKTRPTAINLKWALDR
                     MIDTLKDLCVSERKDVAWALAAEIAEEDVALCEQIGLHGTEVIREIAQKKPAGSVVNI
                     LTHCNAGWLATVDWGTALSPIYKAHENGIPVHVWVDETRPRNQGGLTAFELGSHGIPH
                     TLIADNAGGHLMQHGDVDLCIVGTDRTTARGDVCNKIGTYLKALAAHDNHVPFYVALP
                     SPTIDWTIEDGKSIPIEQRDGKEQSHVYGINPQGELSWVNTAPEGTRCGNYAFDVTPA
                     RYITGFITERGVCAASKSALADMFADLKSKALQGEQH"
     gene            complement(9436..10695)
                     /locus_tag="ISB30_003890"
     CDS             complement(9436..10695)
                     /locus_tag="ISB30_003890"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HAP1991474.1"
                     /translation="MTDSIPSGYKPLTCDTLPGYLSSRLTPSCEPGGLPEEWKVSEVG
                     DGNLNMVFIVEGTHKTIIVKQALPWLRAGGEGWPLSLSRAGFEYNVLCQEAKYAGHTL
                     IPQVYFYDPEMALFAMEYLTPHVILRKELINGKKFPKLAEDIGRFLAQTLFNTSDIGM
                     SAEQKKALTAEFALNHELCKITEDLIFTEPYYNAERNNWTSPELDDAVHKAWADVEMI
                     QVAMRYKYKFMTEAQALLHGDLHSGSIMVTDTDTKVIDPEFGFMGPMAFDIGNYIGNL
                     LLAYFSRPGWDANEQRRADYQEWLLQQIVQTWSVFTREFRQLWDNKTQGDAWPTEMYQ
                     QNRAALEDAQDQFFATLLEDSLVNAGMEMNRRIIGFAGVAELKQIENTELRAGCERRA
                     LTMARDLIVNARQFKNMDSVIQSAKVK"
BASE COUNT         2727 a         2675 c         2667 g         2693 t
ORIGIN      
        1 ttccatgagc aagaaggagc cattatggtt cgcaaagggt gtaattcatt agtccgtgct
       61 gaaaaaatat taacccacat tgcatgggtt ggtatggcaa gttatatgga gttattaaat
      121 aaattccagt accccaaaag cagcctgctt aatttattga atgtaatggt ggagtgtggt
      181 ttcttaatta aaaacaaaaa cggctactat tcactgggaa taaaaaacta cgaattgggt
      241 tgtcaggcgt tacaccgtca aaatatattt gaggtgacta agcggccaat gcaggagttg
      301 tcgctgaaaa gtggactggt ttgtcatctt ggggcgatgg agagcatttc ggctatctat
      361 ctcgacaaga tcgaaagtcc ggactctgta ccgacgagca aaagctggat tggcaaaaaa
      421 attgagttgc atattaccgc gttgggtaag gcattattag cctggaagac acgagaagaa
      481 ctggattatt ttttagaagc actcacgtta actccacata cgcgcaatac atttaccgat
      541 aaaaaattgt ttctggaaga attacaaaaa acacgccttc ggggatgggc catagacaac
      601 gaagaatcaa cctatggggc ggtatgttta agtatgccgg tattcaatat gtataacaga
      661 gttaactatg caatctcact ttctggcgat ccggtagttt attcaggaaa taagatagac
      721 agctatctgg aattgctccg gaaatgcgct gagcaaatat catatgggct gggatacaga
      781 aacgaaaatg agcatttacg aaaaggaaac tgaggtaatg aaaaaattca gcggcattat
      841 tccaccggta tccagcacgt ttcatcgtga cggaaccctt gataaaaagg caatgcgcga
      901 agttgccgac ttcctgatta ataaaggggt cgacgggctg ttttatctgg gtaccggtgg
      961 tgaatttagc caaatgaata cagcccagcg catggcgctc gccgaagaag ctgtaaccat
     1021 tgtcgacggg cgagtgccgg tattgattgg cgtcggttcc ccttccactg acgaagcggt
     1081 caaactggcg cagcatgcgc aagcctacgg cgctgatggt atcgtcgcca tcaaccccta
     1141 ctactggaaa gtcgcaccac gaaatcttga cgactattac cagcagatcg cccgtagcgt
     1201 caccctaccg gtgatcctgt acaactttcc ggatctgacg ggtcaggact taaccccgga
     1261 aaccgtgacg cgtctggctc tgcaaaacga gaatatcgtt ggcatcaaag acaccatcga
     1321 cagcgttggt cacttgcgta cgatgatcaa cacagttaag tcggtacgcc cgtcgttttc
     1381 ggtattctgc ggttacgatg atcatttgct gaatacgatg ctgctgggcg gcgacggtgc
     1441 gataaccgcc agcgctaact ttgctccgga actctccgtc ggcatctacc gcgcctggcg
     1501 tgaaggcgat ctggcgaccg ctgcgacgct gaataaaaaa ctactacaac tgcccgctat
     1561 ttacgccctc gaaacaccgt ttgtctcact gatcaaatac agcatgcagt gtgtcgggct
     1621 gcctgtagag acatattgct taccaccgat tcttgaagca tcagaagaag caaaagataa
     1681 agtccacgtg ctgcttaccg cgcagggcat tttaccagtc tgaggagaaa ctcatgtctg
     1741 ttcgcaatat ttttgctgac gagagccacg atatttacac cgtcagaacg cacgccgatg
     1801 gcccggacgg cgaactccca ttaaccgcag agatgcttat caaccgcccg agcggggatc
     1861 tgttcggtat gaccatgaat gccggaatgg gttggtctcc ggacgagctg gatcgggacg
     1921 gtattttact gctcagtaca ctcggtggct tacgcggcgc agacggtaaa cccgtggcgc
     1981 tggcgctgca ccaggggcat tacgaactgg acatccagat aaaagcggcg gccgaggtta
     2041 ttaaagccaa ccatgccctg ccctatgccg tgtacgtctc cgatccttgt gacgggcgta
     2101 ctcagggtac aacggggatg tttgattcgc taccataccg aaatgacgca tcgatggtaa
     2161 tgcgccgcct tattcgctct ctgcccgacg cgaaagcagt tattggtgtg gcgagttgcg
     2221 ataaggggct tccggccacc atgatggcac tcgccgcgca gcacaacatc gcaaccgtgc
     2281 tggtccccgg cggcgcgacg ctgcccgcaa aggatggaga agacaacggc aaggtgcaaa
     2341 ccattggcgc acgcttcgcc aatggcgaat tatctctaca ggacgcacgc cgtgcgggct
     2401 gtaaagcctg tgcctcttcc ggcggcggct gtcaattttt gggcactgcc gggacatctc
     2461 aggtggtggc cgaaggattg gggctggcaa tcccacattc agcccttgcc ccttccggtg
     2521 agcctgtgtg gcgggagatc gccagagctt ccgcgcgagc tgcgctgaac ctgagtcaaa
     2581 aaggcatcac cacccgggaa attctcaccg ataaagcgat agagaatgcg atgacggtcc
     2641 atgccgcgtt cggtggttca acaaacctgc tgttacacat cccggcaatt gctcaccagg
     2701 caggttgcca tatcccgacc gttgatgact ggatccgcat caacaagcgc gtgccccgac
     2761 tggtgagcgt actgcctaat ggcccggttt atcatccaac ggtcaatgcc tttatggcag
     2821 gtggtgtgcc ggaagtcatg ttgcatctgc gcagcctcgg attattgcat gaagacgtta
     2881 tgacggttac cggcagcacg ctgaaagaaa acctcgactg gtgggagcac tccgaacggc
     2941 gtcagcggtt caagcaactc ctgctcgatc aggaacaaat caacgctgac gaagtgatca
     3001 tgtctccgca gcaagcaaaa gcgcgcggat taacctcaac tatcaccttc ccggtgggta
     3061 atattgcgcc agaaggttcg gtgatcaaat ccaccgccat tgacccctcg atgattgatg
     3121 agcaaggtat ctattaccat aaaggtgtgg cgaaggttta tctgtccgag aaaagtgcga
     3181 tttacgatat caaacatgac aagatcaagg cgggcgatat tctggtcatt attggcgttg
     3241 gaccttcagg tacagggatg gaagaaacct accaggttac cagtgccctg aagcatctgt
     3301 catacggtaa gcatgtttcg ttaatcaccg atgcacgttt ctcgggcgtt tctactggcg
     3361 cgtgcatcgg ccatgtgggg ccagaagcgc tggccggagg ccccatcggt aaattacgca
     3421 ccggggatgt aattgaaatt aaaattgatt gtcgcgagct tcacggcgaa gtcaatttcc
     3481 tcggaacccg tagcgatgaa caattacctt cacaggagga ggcaactgca atattaaatg
     3541 ccagacccag ccatcaggat ttacttcccg atcctgaatt gccagatgat acccagctat
     3601 gggcaatgct tcaggccgtg agtggcggga catggaccgg ttgtatttat gatgtaaaca
     3661 aaattggcgc ggctttgcgc gattttatga ataaaaactg aaagcataaa taaaaacatc
     3721 cagacacctg aattatccga ctctgcatca ttcgttattt gctcagtcgg ataatgatgg
     3781 gtcgctaccc tgttatgaga ggacgttatg ccactaatta tcgttgtggc agggattgct
     3841 ttactcctgc ttttaaccat aaaaattaag cttaatacgt ttgtttcgtt aattattgtc
     3901 tcgattgctg tcgccatcgc cagtggtatg gatttgagta aagtcgtcac ctcagtcgaa
     3961 tccgggcttg gcggtacgct gggccatatt ggtttgatat ttggcttcgg tgttatgctc
     4021 ggtcgcttgc tggccgatgc gggtggtgcg cagcgaatag cgctgaccat gctgaattat
     4081 ttcgggaaaa ataaacttga ctgggcagtg gtctgttcgg cgtttattgt cggtatcgca
     4141 ctttttttcg aagtcggttt aattcttctg gtacctattt tattcgctat tgcccgtgaa
     4201 gcaaaaatat caccgatgtt tatgtgtgtg ccgatgcttt ctggtttgct ggtcgcacac
     4261 ggttttttgc cgccgcaccc cggcccgact gttatcgcca gggaatatgg cgctgatgta
     4321 ggattggtac tgatatacgg tatcattgtc ggcattccaa cctttattct ttgcggcccg
     4381 gtattgaata aattctgcca gcgcattatt ccggacgcct ttaaaaaaga ggggaatata
     4441 gcctctctcg gcgcgacccg ccgattcagc gaaagcgaaa tgccaggctt tgggatcagt
     4501 tttctcacgg caatgctgcc ggtgatcctg atggcggttg tcaccatcat ccagatgact
     4561 catgccaaaa gtgccgcgga ttctggctta ttttacaacg tgatactgtt cttaggtaat
     4621 tcgacgatcg ccatgctgat ttcactgctg ttcgcgatct ataccatggg cctggggcgc
     4681 gggaaaacaa tccctgacct gatggattct tgcggaaaag ctatcgcggg tattgccggt
     4741 ctgctgctga ttatcggtgg tggtggtgca ttcaagcaag ttctcatcga ctctggcgtt
     4801 ggtcagtata tttccaccct ggtttcgggt atggatatta acccgatctt gatggcctgg
     4861 ggcgtggccg cattcctgcg tatttgtctg ggttcagcca ccgtcgcggc gatctctacc
     4921 gccggtctgg tcatcccatt gttggcagta catcccaata ccaacctggc cttgattacg
     4981 ctggctaccg gtgccggatc ctgtatttgt tctcacgtta acgatgccag tttctggatg
     5041 attaaagact tttttggcct gaccaccaaa gaaacgctgt tatcctggac gttgatgtcc
     5101 acactcttat ccatcagtgg gctgattttt attctgctcg ccagcttagt tttataatcg
     5161 ggttcttgcc agtgagccaa cctgttgttc actgtccccc ataatttagt gagtgacggc
     5221 aaccgtctgc acggtgacga atcgctggcg atcaaaaagg ggcgatattg aaccgtcgat
     5281 tatgcttgat gggtgtactc tcagtaatac tgtaaagccg gagcatgtct ccgactttac
     5341 agtatggggg aacagcaggc gatcgctcaa atggtatgct ggtttatttc atgtaaaaga
     5401 ataaaatatc tgtataaaat catgccactg gcaatctgca tcaactctcc gggtaaacag
     5461 ggttaagatg agcatttccc cggtttaata caggtataca aaagtggata gagatcaaac
     5521 aaacagcagc ctgttcaatg atgatcccgt acttcatgca acatggctgt actatcagga
     5581 aggcaaaagc cagactgaag ttgcagctat catgggggtg tcccgcgtca cggttgtcaa
     5641 atacctgcaa acagcccggg aaaatggact ggttcatatc aacctggatg tgaatgtctt
     5701 tggctctatc gacgctgctt tgcaaattcg cgataaattc aatctccaga gagtgatcat
     5761 cgtgccggat ggcgaacatg ccggaaaacg tgatgataca aagctgatgc ggactcgcct
     5821 ttcacgtgcc ggtggcatgt atctgaatca ggtgattgag aatggtgatg tgctgggggt
     5881 tgcctgggga agaacaatcc atcagatgag caaaaccatg acgccgaagt catgtaaaaa
     5941 tgtcaccgtt atacagatgc tcggttccat gccatctcag ccagacctga cgatcattga
     6001 atcctcatcg caaatcgcct acaagctttc aggccgtgtc gcctcgctgc acgtgcccgc
     6061 cgtggtatca agcgccagac tggccatgga gttacaggca gagcctatta ttcgatcgaa
     6121 ttttgacgtt ctgactcgct gtacgaaagc cttttttgtc gtgggtaacg cgcttgatga
     6181 aaatcccctg attcgggtgg gcgtgctgaa taagaaggaa atgcagacgt atcgggattt
     6241 aggcgcagta ggtgtcattt gtggtcgctt ctatgacaag gaagggatgc ccgtcgtggc
     6301 tgatgttgac cagcgtattc tgggaatcag tctggctcag ctaaggcaga ttgaacggaa
     6361 gatcttcctt gctggtgggg aaagaggcta tgacgcaacg ttgggtgccc tgctgggagg
     6421 atatgtcacg gacctgattg ttgatgaagg aacggcagag tttttgctgg cgtgtgaatt
     6481 accacactaa aagaatgaac aactgcccca tgtggatatg gggcaggatt ttattgatta
     6541 gcggcgataa taagaacaat acccacaatg tatgaaataa acatggtcag cagcagttta
     6601 ttggttccgg ctggcgcact ggcaaattct ctccagatga agactcccca gatcacggca
     6661 atcattgtgg ccccctgtcc cagaccgtag gaaatggcat accccgcctg accagacgca
     6721 atcagactga atgccagacc gacacaccaa atcataccgc ccagccagcc gcaaacatga
     6781 tcccgtaagc tcccggaaaa atacattttc ccgttgaccg gttctcctga aataggtttt
     6841 ttcatgacca gcgtgttaag gacaaagttc gacaagaaca gccccacggc aaacagaaca
     6901 agtgctgagt aaggcgtcat cagaccgctg gcaggctggc tgaaattgtc agacatggag
     6961 tccgccaaga agcggaaaaa ccatcccatg atgatacccg ccagaatggc agtaatgaga
     7021 cctttattac ggcgagattt atccgcctcc tgcgtgacac gaccataggc tatcgcggta
     7081 aaaataatgg cgctcacaac gcaggcgaca ccgaggaaca gaatgagcgg atcgccctgc
     7141 ggattgccga tataggtggt gatcacaccc agagccagtg ccagccccac acccacagga
     7201 aaagctaccg ccataccggc aagattaata gaagcgacca gaagaatatt ggaaatattg
     7261 aagataatcc ctgccagtat tgccgacatc agactactgc tgctcgcctg ctgaatatca
     7321 ggtataaaac tgcgaccggc ttcgcccata gaacccagcg tgaaggcaaa taacaaagaa
     7381 cacagcaata atccaattga ataatcccaa tagaatagag ggaactccca ttttttattg
     7441 ctgacaagtt tagttgtatt cgcccatgaa ccccagcaaa tcatggtaat aaaacacatt
     7501 attatggcga cagcatacga ttccacgata aacataaata actccttgtg gtagttataa
     7561 tcaatcgttg tggacagagg gaatagcagc cattctgcga atggctgtca tatgttatta
     7621 attaaaataa agctgtaatt acgctttcaa accatacgtt ttgaattttt caaggacgat
     7681 ggtcatttcg gcttcagaca gcacgggaac atcgtgatgt aatccgccac atttgatgta
     7741 taaatcagcc agcgtctcgg tttcacccgc cagccacaac gtcttttcca gcgtgacgtc
     7801 catggccagc ataccgtggt gctgcatcag caaggactta ctttctctga ttcctttaga
     7861 tacaccgtcg gccagttcag gactgccaaa ggtataatag ggaatacagg gaatatgatc
     7921 cgtccccgat accgccacca tataatgaat ggccggaatt gaatgattat gtattgccac
     7981 cgccgtggaa ttcaccgcat gcgtatgtat gacagcattc aattctggtc tggctttata
     8041 acacgccaga tgaaataacc attcacttga tggtatttta cccgcttcag gtattccctt
     8101 atcgtctaca aagacaatca tgtccggcgt catctttgaa taagcgatcc cactcggcgt
     8161 aatcaacatg ccaccggtat acctggcact cacattacct gatgttcctt gattaagacc
     8221 tgacgcattc atttcccgac aagtggaaat gattttttct gctaacttaa tccgttccat
     8281 tcctgatcct taatagcaat ggccgccgtt ttaatgctgt tccccctgga gtgctttact
     8341 cttcaggtca gcaaacatat ccgccagcgc tgacttactg gcagcacaga ctccccgctc
     8401 agtaatgaag ccggtaatgt atcgggcagg cgtgacgtcg aaagcgtagt tcccacaacg
     8461 ggttccttca ggggcggtat tgacccagct caattctccc tgagggttta tcccatagac
     8521 atgggactgc tcttttccgt cacgttgctc aatcggaatg ctttttccgt cctcgatagt
     8581 ccagtcaata gtgggagaag gtaacgcgac gtagaaggga acatggttat catgggccgc
     8641 cagcgcctta agataggtac caattttatt acagacatcc cctctggccg tggttcgatc
     8701 ggtcccaaca atgcagagat caacgtcacc gtgttgcatc aggtggccac ccgcgttgtc
     8761 ggcgatcagg gtgtgaggaa taccgtgtga gcccagttca aatgccgtga gtccgccctg
     8821 attacgtggc cgcgtttcat ccacccagac gtgaacaggg attccgtttt catgcgcttt
     8881 gtagatggga gaaagcgcgg ttccccagtc tacagtcgcc agccagcctg cgttacaatg
     8941 tgtcaggata ttgaccacac tcccggccgg ttttttctgg gctatttcgc ggatgacttc
     9001 tgtaccatgt aatccaattt gttcacacaa cgcgacatct tcctcggcaa tttctgccgc
     9061 cagcgcccag gccacatcct tgcgctctga tacacagaga tccttcagtg tgtctatcat
     9121 cctgtcgagg gcccatttga gattaattgc cgttggtcgg gttttgataa gaaggtcata
     9181 gtaacgctgc agacccatgt cactggcatc gtgattcatt cccagcgcga tgccataggc
     9241 tgcaacgaca ccaataagcg gagcgccacg aacccacatc tcctgaatag ccgtagcggc
     9301 catttccgca gaggtaagcg ccaccacctc gaacttaaaa ggcaacttag tctgatcgat
     9361 gatttctacc gcttttccat ccccggaaac ccagaccgta cgatagtgtt tacctttaat
     9421 attcataatg acctcttact taaccttcgc agactggatg acggaatcca tatttttaaa
     9481 ctggcgggca ttgacgataa gatcgcgcgc catggtcaat gcacgtcgtt cacatcctgc
     9541 gcgaagctct gtattttcaa tctgtttcag ctcggcaacg ccagcaaaac caatgatccg
     9601 gcgattcatt tccatgccgg cattcaccag ggaatcttca agcagcgtgg caaaaaactg
     9661 atcctgtgcg tcctcaagag cggccctgtt ctgttgatac atttctgtcg gccacgcgtc
     9721 gccctgtgtt ttgttgtccc agagctggcg gaactcccgg gtgaaaacgg accaggtttg
     9781 gacaatctgc tgaagcagcc attcctgata gtcagcgcga cgttgctcat tcgcatccca
     9841 cccagggcgt gagaagtacg ccagcaggag gttgccaata tagttgccga tatcaaacgc
     9901 cattggcccc atgaaaccga actccggatc aatcactttg gtatccgtgt cggtcaccat
     9961 gattgagcct gaatgaaggt cgccatgtaa caatgcctgc gcttctgtca taaatttgta
    10021 cttataacgc atggcaacct ggatcatctc tacatcagcc caggccttat ggacggcatc
    10081 gtccagctca ggagaagtcc agttattccg ttcagcgttg taatagggct ctgtgaagat
    10141 cagatcttcc gtaattttgc acagctcatg attcaacgcg aactcggcag taagcgcttt
    10201 tttctgttct gctgacatgc caatgtcaga cgtattgaag agagtctgtg ctaaaaatct
    10261 gccgatatct tcagctagtt tagggaattt tttaccgtta atcagttcct tacgcagaat
    10321 cacgtgagga gtcagatact ccatggcaaa cagcgccatt tccgggtcgt aaaaatagac
    10381 ctgcggaatc agtgtgtgac ccgcgtactt ggcttcctga cataagacgt tgtactcaaa
    10441 gcccgcacgg ctcagagata aaggccatcc ttcgccccct gcacgaagcc agggcagagc
    10501 ttgttttaca atgatggttt tatgtgtccc ctcaacgata aacaccatgt tcaggtttcc
    10561 gtcccccact tctgaaactt tccactcttc aggtaaccct cccggttcgc atgaaggggt
    10621 cagtctggac gagagataac ccggcagcgt gtcacatgtc agcggcttgt aacctgatgg
    10681 aattgaatcc gtcataacag ttctccttgt ctacacagaa tgataatcaa cttgtcatgt
    10741 gacaactata cattatcaaa tg
//
LOCUS       DACADF010000149        10606 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.151, whole genome shotgun sequence.
ACCESSION   DACADF010000149 DACADF010000000
VERSION     DACADF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.151"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..1349)
                     /locus_tag="ISB30_003891"
     CDS             complement(<1..1349)
                     /locus_tag="ISB30_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAP1991475.1"
                     /translation="MTSKNFALITAMTQAELTQKVNEHLAKGWHLQGETRVAYEPGTP
                     WYLMQAMVADGTTDISPDSPQHGSVPEWYYVVVLAGQSNAMSYGEGMPLPDSYDAPHP
                     RIKQLARRNTVTPGGKACAFNDIIPADHCLHDVQDMSALNHPNADLSKGQYGCVGQGL
                     HIAKRLLPYIPQNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQD
                     LILRTKAALQKNPKNMLLAVCWMQGEFDMSAATYSQQPPLFTAMLKQFRADITEFNTQ
                     CHGGRAASVPWICGDTTYYWKNTYGTQYDTIYGAYKNRESDNVFFVPFLTDGSGNNTS
                     TNAPTEDPDAASEGYYGSASRTNKNWVSSNRQTHFSSWARRGIIPDRMATAILNVAGR
                     TLAFISGKAPEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLS"
     gene            complement(1488..1883)
                     /locus_tag="ISB30_003892"
     CDS             complement(1488..1883)
                     /locus_tag="ISB30_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HAP1991476.1"
                     /translation="MKLWPTLGVAFLLITSMRLSWSLGRENARNEAQASTLKSTVDTL
                     NIISAGVQDMQQVLAQLRAENQQRNQDGEVRREQLRNDIAKDECAHALPDARFTDRLR
                     RHAERARASAVSPAYTADADHAGNASPLP"
     gene            complement(1880..2272)
                     /locus_tag="ISB30_003893"
     CDS             complement(1880..2272)
                     /locus_tag="ISB30_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP1991477.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVTTGKSQTMNSRHLTGHAVDVVAYVGNQVSWEWPLYEKIAAAFRQASQEL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(2269..2592)
                     /locus_tag="ISB30_003894"
     CDS             complement(2269..2592)
                     /locus_tag="ISB30_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP1991478.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGIPEDAVTFFGASIGFMGAEKARDKVIAIFDRRV
                     KERNE"
     gene            complement(2595..2795)
                     /locus_tag="ISB30_003895"
     CDS             complement(2595..2795)
                     /locus_tag="ISB30_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1991479.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(2795..3289)
                     /locus_tag="ISB30_003896"
     CDS             complement(2795..3289)
                     /locus_tag="ISB30_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HAP1991480.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAIAHVTRELEAWQQEQQAAGYQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILGRPRMF
                     AELC"
     gene            complement(3391..4191)
                     /locus_tag="ISB30_003897"
     CDS             complement(3391..4191)
                     /locus_tag="ISB30_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP1991481.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAACTAHEEVLYRLRLAQA
                     RLKAIQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLWEI
                     IANQDMPDEVRAKLCKACAFARRGLTDADNMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            complement(4237..5289)
                     /locus_tag="ISB30_003898"
     CDS             complement(4237..5289)
                     /locus_tag="ISB30_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HAP1991482.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNSSYDGIKRRNPNNVIDMK
                     SRQYQCEQVNYDTFISYPQLDTWAAHSDFQSRISTQIARQVALDRIMIGFNGTSHAYE
                     SDFHTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLAVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            complement(5313..6149)
                     /locus_tag="ISB30_003899"
     CDS             complement(5313..6149)
                     /locus_tag="ISB30_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HAP1991483.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            6304..8055
                     /locus_tag="ISB30_003900"
     CDS             6304..8055
                     /locus_tag="ISB30_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP1991484.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFMVMEF"
     gene            8055..9101
                     /locus_tag="ISB30_003901"
     CDS             8055..9101
                     /locus_tag="ISB30_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP1991485.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNSYALLDEKTAP"
     gene            complement(9116..9640)
                     /locus_tag="ISB30_003902"
     CDS             complement(9116..9640)
                     /locus_tag="ISB30_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991486.1"
                     /translation="MVINYKQLREKREQVKESFRRNEDLTPLVRLAQGIVDAYEISLE
                     LPSQTWTDSDGNRQHYVSCGLEAAEGFRRMPLSQIPAATPKARGSNDERKLTFSIETV
                     VDDTPGEVAFVHTPVSIAMYNDEIQVRVNNNIVPLKEGNSPYTTVCEAIQYYVLSEID
                     NLKPDGTQKMVQLW"
     gene            10364..>10606
                     /locus_tag="ISB30_003903"
     CDS             10364..>10606
                     /locus_tag="ISB30_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HAP1991487.1"
                     /translation="MNPITLQIISNAIVLLGVLVAIGTIIYNVRTAKKTQTANFLFES
                     RQDMQYIESLHTLKQVHRSGKSFRSYVFPCDGCIITD"
BASE COUNT         2277 a         2826 c         2879 g         2624 t
ORIGIN      
        1 tctgacaact gaattccgcc attcttaatg ctccagccct gcgcagcagc atctccggct
       61 gtcggcaaca gggagattgt gcgtacggat gcatcttcag acggccccga tggcgtgtcg
      121 ccgccgggcg agggtttgat ttccggtgcc ttaccactga tgaaggctaa ggtgcgaccg
      181 gctacgttca gaatagcggt tgccatacga tcgggaataa tgccacgacg cgcccatgag
      241 ctgaaatgcg tctggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
      301 taatatccct cacttgcagc atccggatct tccgttggtg cgttggtgga ggtattgttg
      361 ccactaccat cggtcaggaa cggcacaaag aaaacgttgt cgctctccct gtttttgtac
      421 gccccgtaaa tggtgtcgta ctgcgtgccg taggtgtttt tccagtaata cgtcgtgtca
      481 ccacaaatcc atggcacact tgccgctctg cctccatgac actgcgtgtt aaactcggta
      541 atgtccgcac gaaactgttt cagcatggcc gtaaacagcg gaggttgctg tgagtacgta
      601 gcggcgctca tgtcaaattc gccctgcatc cagcatacgg ccagcagcat gttttttggg
      661 tttttctgca atgcggcctt cgtgcgcaaa atcaggtcct gatataaagg tttacccaca
      721 ccccagcgag ccgaatcctg actggctcct gtggactcgc tgaatgtccc ctccgcgccc
      781 tgggtgaatg ccgaaccacc acgacagcat ggaaccagta aaatcccggc attctgtgga
      841 atgtaaggca acaggcgttt ggcaatatgc aagccctgtc cgacacagcc atactgccct
      901 ttgctcaggt ctgcattcgg gtgattcagt gcgctcatat cctgaacatc atgcaggcag
      961 tggtctgccg gaatgatgtc gttaaatgcg catgctttac caccgggagt cactgtgttg
     1021 cgacgggcca gttgcttaat gcgtgggtgg ggcgcatcgt aagaatccgg cagcggcatt
     1081 ccctcaccat atgacatggc attggattga ccagcaagta ccaccacgta ataccactcc
     1141 ggcacgctgc cgtgctgggg ggaatcaggt gagatgtctg tagtgccatc ggccaccatt
     1201 gcctgcatta gataccacgg ggtgccgggt tcgtaggcaa cccgcgtctc cccctgaaga
     1261 tgccaccctt tcgcaagatg ttcattcacc ttttgagtca gttcagcctg tgtcatggct
     1321 gtaatcagtg caaagttctt actggtcatt gtgttactcc cgtctggtgt gtcagttcgg
     1381 ctattctctt atccagttca gcaatggccc gtttatccgc gttacaggtt tccagtgcat
     1441 ccagaaggcg gtcgccccat attccgaggt ttccccatgt gggtgggtca gggaaggggg
     1501 gaggcgttac cggcatggtc agcgtctgcg gtataagccg gactgacggc gctggccctg
     1561 gcgcgttctg cgtgcctgcg caacctgtca gtaaaacgag cgtcaggcaa agcgtgggcg
     1621 cattcatctt ttgcaatatc gttgcgtagc tgttcacgtc ttacctctcc gtcctgattg
     1681 cgttgctgat tttccgcgcg gagctgagcc agcacctgct gcatatcctg taccccggcg
     1741 ctgatgatat tcagtgtgtc gacggtactt ttcagggtgc tggcctgcgc ttcgtttctg
     1801 gcgttctccc ggcccagcga ccacgacaga cgcatggatg taatcagaag gaaagcgacg
     1861 ccaagcgtgg gccagagctt catgccggat aggctccgtg tggtaactga aaatgcggtc
     1921 cgtctttcag ggtcttccag tcgccgcccc attccaccgg aatattcagt tcctggctgg
     1981 cctgtctgaa tgctgctgcg attttttcgt acagcggcca ttcccatgac acctggttgc
     2041 cgacataagc cacaacatcc acggcatgcc ctgtgagatg gcgactgttc atggtctggc
     2101 tcttgcctgt ggtcacgagc tgcttctgac gataacgact gcgcaatcct tcggtgatac
     2161 caaaatccac ttccgagatt tccagcgccc gtcgggtcac tttcaccaga tcaggattta
     2221 caccctgcaa attcttttcg ctccggctgc tgaatttaaa tgtgttgctc attcgttcct
     2281 ctccttcacc ctgcgatcaa atattgcaat aaccttgtcg cgtgctttct ctgcgcccat
     2341 aaaaccgatt gaggcaccaa aaaacgtcac tgcatcctca gggatcccga agaagcgcag
     2401 cgacccggcc acggccatgg caagaacgcc gcacgccagc gatcccgtta cggtctgaac
     2461 cagtgttcgt ccgtcataaa gactcatcag cgcggaaatg ctgaccgccg cgcctactgc
     2521 atacaccgtt ggcaggtggt caaagagcca cgcaataacc tgctctgtga tccctgtttg
     2581 aatggtgctc actgctactc cccccacaac tgaatcattt ctcgtttctt cttctccggc
     2641 tccggcatct ccacttcctg cccggcgtcc agaaatacct gctgacagag tccggggttg
     2701 gcatccagca ccttttcggt gacgccctgt gtcgtgccgt agtaccggaa acagagcgaa
     2761 tccacggtgt cgccttccag tgccttcact ttcatcagca cagctccgca aacattcgcg
     2821 gtcgtcccag aatgtcagag atggcccagc tcacatcgcg ccacaaatcc gatgtctgta
     2881 tatccagtgc gtccgcccgg cggtcgccct tgtccgttgt gtccgcatcg cggtaacgct
     2941 ccagaatcag ggcgcgtgtg gcggtataaa cagcattgcg ccagtgccag agattgacgc
     3001 tttctccgtt aattacaggg gccggaacat cggccagcgt ctgatagcca gccgcctgtt
     3061 gttcctgctg ccatgcttcc agctcgcggg taacgtgtgc tatggccccg gtggcggtat
     3121 gcagcaggcg ggaggtggtc acgcggcccg gcagtcgtac cgccagacgc agctcgcgca
     3181 gcacaatatc cggccagaat gcacccgctg aaatacgggt atcgccatca tcggtgtcgg
     3241 tgatgtcgtc ctctgcgggg ccggggttag ttctggcaac catactcatg gggttcactc
     3301 ctgaaaaaat cgggcggtgg gtgcgcggtg taaacggtca cggagtcaaa ccggaacacc
     3361 gcgcacgccg cccgctgacg gggtcagtcg ttaaccgcgc tttgccttct gcgtcgcggt
     3421 ggtttttcgt gttgcaggct tccgcgttgt ctttttactt ttgctgcttt cgtcctgcgc
     3481 ctgctgtgcg ctggcgtctt ctggttcggc tgcggaatcg gcttttttca gggcgcggga
     3541 aagggttgca atctcgcgtt tcacacctgc gttcgggttc aggtgcatcg cttcgcgcag
     3601 cagcttcagt gatgaggcca tgttgtctgc atcggtcagg ccacgacggg caaaggcaca
     3661 cgccttgcat aatttggcgc gcacttcgtc cggcatgtcc tggttggcga taatttccca
     3721 aagtgtgtcc agtggttcga taaaggcgga taaatccgcg tcggcatccg tcccagcctg
     3781 cgtcagtacc gggttgcaga tttcttcggt cagtaccgtg gcagcagtac ggccaaagtt
     3841 atccggcatg atgaggttgt gacggaccac atatgcacca atacgcagcg ccagcggaag
     3901 atcgccgcag tcaatcgccc acaccatcag cgtggcaatt acttcgtcct gttgcccgcc
     3961 gtcagcctcc agcgttccct caatccagcc ggaaaagtcc ggcaacaact cttttttgat
     4021 ggcggctttc gcgcttctgg cctgtatggc ctttagcctg gcctgtgcca gacgcagacg
     4081 atacagcacc tcttcatgcg cggtacacgc ggcgtggtcc acaccttcat tcgcccggcc
     4141 tgcgcgctgt gccatcacgt tctgccagtg ttgttgtgca ggagtaatca ttttttctct
     4201 ccgttacagg cgggcatgat gcccgccgtg agttgattag ctgtcggcga acttcaggcc
     4261 agtgaccatc gcgcacttgc catagtcttc aacgacataa gcgtcattga tggactggta
     4321 ggtggcgatg cggttgtatt ccggctcgtc tttcatcagg cgacgcattg aacctttctg
     4381 ccagtaaatc gacaggttgt tgaacgaggt gataagcatc gttgcatccg ggaagaacgg
     4441 cgcaaggaac acgcccagcc cgccaatggt gcgcgatgac aggatgagct gcccggcaag
     4501 taattccgca ttgggattct ggccgctgat gctgttcagc acgggcagac gcagcgagtt
     4561 aaacaggttg cgccccataa tcaccgcgag gtcgtcagct tccttgtgcc attcatccag
     4621 cagggatgag cgcgcatcct gtaccagagc atcggcattc gcatacttac ccgcgtgcgc
     4681 cacagtgttg tccatgttgc gggaggtcag cgtcacgtca ttcattacgc gctcgctggc
     4741 atcggttctg atgtgctcca gccagcccac gttaacgtcc tgaagcagct tgttggtgtg
     4801 aaaatcagac tcgtaggcgt gggatgtgcc gttaaagcca atcatgatgc gatcaagcgc
     4861 tacctgccgg gcgatctgtg tactgatacg ggactgaaaa tcgctgtggg ccgcccaggt
     4921 atcaagctgt gggtacgaaa taaaggtatc gtagttcacc tgttcgcact ggtactgacg
     4981 agacttcata tcgatcacgt tattcgggtt acggcgtttg atgccgtcat aactgctatt
     5041 cgtgcgcgca attggcccgg tggtgtccag gaggattttt tcgcctttct ggtcggtcac
     5101 gccgatcacg ttaattcttt tcgtaaattc ggtactttcc tttgaggcgt tttcaaaacg
     5161 ctgctgcacc gcgggttcca cggtaaatcg cgataccagt gcagataccg ggatattgtt
     5221 aagcgacgcc tgctgcgcca tatagcaacc cagcttgttg cgggtaatat ctgacatcac
     5281 cagattcata aaaaatttgc tcctttgtct tatcagaagt cagccagctg gtcggaggct
     5341 gcgcccgttg cggtgaagcg gttctgcgga tcgccgtcct gcgtgcgcag tttttccttc
     5401 aatgctgtca gctctgtggt cagtgacgtg attttctggc ggtcctgctg atggcgggtt
     5461 tccagcacat taaaacggtc gataatgtcg gcctgtgacg ttgcaacgcc ttccaccgct
     5521 tcctgaattc gggagaaact ggcgtcatcc gctttgcggc cacgaccaat aatccccatt
     5581 acgcggttaa accactgggt gccttcttcc tggcgttgtt ctgccatttc gatgatttca
     5641 gactcgatgg cttcggagat aagcggtgct tcaccctgga tactgttgaa cgtcatcacc
     5701 gcctgacgtt gctgtgccgt gaatttcagg cgctcagtgc ccaggcttgc cggggtgtcg
     5761 gtcatcgcca gcccgaccag ataggcgcgc ccgttaacgg agaactgcgg gtgcagttcg
     5821 atactggaat agattttctt gccgtcagcg acaagctgct tcatgcgctc ggtcggttcg
     5881 atttctgcat acagcgcagt acgtccggcc agcggacctt ccgtaatgtc ttccgtactc
     5941 agtgcggtga catcgcccat tgcggaaaat tcgcttgacg gacatggcga gagatagtgc
     6001 tcaacgttca cgcgggcagc gtaaacatcc gggttgaagt tctcggcggc ttcacgcaga
     6061 tgcaccggac tgatttcacg gccatcaaca gttgatccgg agacagccac gcgaaacttt
     6121 ttgcgggatg tctttttttc attagccata gtttttgccc ctctgactgg ttcttcagtc
     6181 atgatggcaa agcgtaacag gctgatacaa agggcttttg ttgtaagaaa acagtcagaa
     6241 cagagggtta aggagaacag tttcgcgcgc gggtaatctt cctgtaatta ctcaggggga
     6301 gcaatgattc aggacgcttt tgtgcgccag cgtgcgcggc aactttactg gcagggttat
     6361 ccgcccgcag aaatatcacg tctgatggga ataaacccga acacgattta tgcgtggaaa
     6421 aaacgtgacc agtgggatga aacgccaccc gtgcagcgtg tcacgcagtc catcgatgcg
     6481 cgcctcatcc agcttactga aaaacagaat aaaacaggcg gtgactttaa ggaaatagac
     6541 ctgctgaccc ggcagcttaa aaagctgcat gatggccagc cggatgtgat ggccgcagga
     6601 aagaaaggcc gggcgaaaaa actcaaaaat catttcacgc cggaacagat tgccgcactg
     6661 cgggaaaaaa tcatcagcag gctggagtgg catcagcggg gctggtttga ctccctgacc
     6721 ctttgcaggg aagccgggat acgtaacagg atgatcctga aatcccgaca gattggggcg
     6781 acctggtatt ttgcacagga agcactgctg atggcgctgc gtgacgatgt ggcgcaacct
     6841 taccagcgta accagatttt tttgtctgcg tcgcgtcgtc aggcgttcca gtttaaaagc
     6901 attattcaga aggccgcggc tgaagttgat gtggagctga aagggggcga taaaatcatc
     6961 ctctccaacg gcgcagagct gcattttctc ggcacttctg ctgcgtcggc acagtcctat
     7021 acgggcaatt tttattttga tgaatttttc tgggtcagtc gctttgctga actgcgcaag
     7081 gtggctggcg ctatggcaac cctcagcgga ctgcggcgca cctacttctc cacgccatcc
     7141 accgaaacgc acgaggcata cgcctactgg aacggcgacc gctggaacga gaaaaaggcc
     7201 tcgcataaac gccagcgttt ttctgtggac tggaaaacgc tgcataatgg gcttatctgc
     7261 cctgaccgga cgtggcggca aattgtcacg ctggaagatg tggttaatca cggctggaaa
     7321 cacaccgata tcgacgaaat tcgtgatgaa aacaccgaag acgagttcct caatctctat
     7381 atgtgtgagt ttgtccgcga aggggaatcg gcatttaacc tgaatatcct gattggctgc
     7441 ggtgttgacg gatacgacga ctggaaagac tggaaacctt ttgctccccg cccgatgggg
     7501 aatcgtccgg tatggattgg gtatgacgca aacggcagta gtggcaacgg cgacagcggc
     7561 gctgtgtccg tggtggttcc tccggctgtt cctggcggcc gttttcgaac ggtggagacg
     7621 cgacgcgttc aggggctgga gtttgaagaa caggccagag tcattgaaga gttcacgtgt
     7681 cgctacaacg tggaacacat cggcattgat gtgacgggcg ggaacgggga ggctgtttat
     7741 cagatagtga agcggttttt ccctgctgct attccgtaca ccttcacgct gtcatcaaaa
     7801 cggtcgctgg tactgaaaat gctgcaaata atgcgtgccg ggcggtggga atacgatcgc
     7861 gccgaacgcg agctggtcgc ggcctttaac gccgtgcgta aggtgaaaac accgggcggc
     7921 tttatcactt acgaaacgga ccgcgcgagg gggatcagcc acggcgacct tgcgtgggca
     7981 accatgcttg ctgtcattaa cgaaccaatt ggcggcgaag gagaaaacga gcgtttcatg
     8041 gttatggagt tctgatgagc agaaaaaata aaaaagtgcg catgagttca cgcattgatc
     8101 tcgctgatgc gctcaggaaa gaatcatcgc tcagtgcatt cacatttgat ggtccttatc
     8161 gcctgaccgg gcatgacctg ctggacaata tgtactgtgc tgataacggg cggtggtatg
     8221 aaaccccggt ggactggtac ggtctggcaa gagcagcccg gcaaacgtcc tggcatcagt
     8281 ctgcgcttta ctttaagcgc aatgtattac tcggttgcta catcccgcac ccgctgcttt
     8341 cccggcagga tttctcggcg ctggcgctgg actggtttgt gttcggtaac gcattccttg
     8401 agcttcgaag caatatgctc ggcgaaccgc ttaaattacg gcacgccctg gcgaaataca
     8461 tgcgacgcgg aagcgatctt gaatcatggt ggtatgtgca ggatggcaag gacgcgtttc
     8521 agtttcgtcc tggcaaagtg tgccacctga tgaatccgga tattaaccag gaaatctacg
     8581 gcatgccgga atatcttggc gcattactct cggccagcct ttctcattcg gcggacatgt
     8641 tcagaaaact gtactacgac aacggatccc acgccgggtg catcatctac atcggtgcag
     8701 cgcaggtaaa ccgcgaaagc atggactccc tgaaagaaac gctacagggg gcacgtggtg
     8761 gtggtgcgtt taaaaacgtg ctcattcatg cgcccaacgg gggcaaagag ggggtgcaaa
     8821 ttttgccgtt ccagcagatc accgcaaaag atgaattcat gaatgttaag gcggcatccc
     8881 gtgatgatgt gctggctgcg caccgcgttc cgccgcaact gatgggggcg atgccgggcg
     8941 aaaaaagtgc gtttggcgat gtggagaagg cagcgcgggt ttacgcaatt aacgagctga
     9001 tgcccgttat ggaggctatg aagcacatca atgactggct tggcgaagag gtgatccgtt
     9061 ttaactctta tgctcttctt gatgaaaaaa cagccccgtg atggggctgt cctttttacc
     9121 agagttgaac cattttctgg gtgccgtcag gcttgagatt atcaatttca gagagaacgt
     9181 aatattgaat ggcttcacaa acggtggtgt agggtgaatt accttcttta agtggcacga
     9241 tattattatt aacgcgaacc tgtatttcat cgttatacat tgcgatcgaa acaggagtgt
     9301 gcacgaacgc gacttcgcca ggtgtgtcgt caaccaccgt ttcaatacta aaagtcagtt
     9361 ttcgctcatc attgctgccc cgtgctttgg gggtagcggc agggatctgg gataaaggca
     9421 ttctgcgaaa tccttcggct gcttccagtc cgcatgaaac gtaatgctgg cgattaccgt
     9481 cgctatctgt ccaggtctgt gatggcagct ccagcgagat ttcataagca tcaacaatgc
     9541 cctgggcaag gcgtacaagc ggggtcagat cttcattgcg gcgaaagctc tcctttacct
     9601 gctcccgttt ttctcttaac tgcttgtaat taatgaccat aagacagcct ccattgattt
     9661 ctttgctcgt attttgcact tatgaagtat ggtcggcaag gtgccgtatc gctgacgcgc
     9721 ttcgcttgtc tgctgcttcg ccggggcata aaaaatttat gccccggctc tccagctcct
     9781 gtatcaatca gataatttca cgacgccttc cagtttatcg ccaccatcga cggtcagact
     9841 cttacgcaat tccaccgcgt tgactgcatg ttctcgctgc ctcagtgcga ttttgacggc
     9901 cttacctttc accccatcaa atcaaaagcc ctcacgcctt tttcatgctc agcgtgagaa
     9961 atatggccat tctgttgtgt ctctgcgaca tcgttcaggg aatgctattt accccctgaa
    10021 acgcgggctg ttcccccgtc acctgcgcgc agaaaaagcg cgtttttttg tgcacgcacg
    10081 gatccttgac ggatccagcc gccatgcggg ccggaagggt aaaaagtcgt tcaaaaaaat
    10141 tgtgcaaatt tgtgcacttt tgtgcaggta agcatgccta tatatttgac cgtggcttgc
    10201 ctttgatgta gtttatacgt cacaaaaacc acaaaacgtg gttatgattt ctatgagggg
    10261 gttgatatga aaaaaatgta tgatgaattt gatggtttct aagctacgtt gacctctaat
    10321 gaggcgggga tttgctcccc gcctttttta tggatgaaat aggatgaacc caataacact
    10381 acaaatcatc agcaatgcta ttgttctgct tggcgttttg gttgccattg gaaccattat
    10441 ctacaatgtg cgtaccgcaa aaaaaactca aacggccaac tttctttttg agagtcggca
    10501 agatatgcaa tatatagagt cattgcatac cctgaaacaa gtgcatcgtt caggcaaatc
    10561 atttcgttct tatgtttttc cttgtgatgg ttgcataata acagat
//
LOCUS       DACADF010000150        10461 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.152, whole genome shotgun sequence.
ACCESSION   DACADF010000150 DACADF010000000
VERSION     DACADF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.152"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            98..2542
                     /gene="dmsA"
                     /locus_tag="ISB30_003904"
     CDS             98..2542
                     /gene="dmsA"
                     /locus_tag="ISB30_003904"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HAP1991488.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            2553..3170
                     /gene="dmsB"
                     /locus_tag="ISB30_003905"
     CDS             2553..3170
                     /gene="dmsB"
                     /locus_tag="ISB30_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAP1991489.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            3172..4035
                     /locus_tag="ISB30_003906"
     CDS             3172..4035
                     /locus_tag="ISB30_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAP1991490.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMLGLWVLMGIGFISSMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCVWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAN"
     gene            complement(4071..4697)
                     /locus_tag="ISB30_003907"
     CDS             complement(4071..4697)
                     /locus_tag="ISB30_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP1991491.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            5011..6159
                     /locus_tag="ISB30_003908"
     CDS             5011..6159
                     /locus_tag="ISB30_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1991492.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGIGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQTENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            complement(6256..6996)
                     /gene="pflA"
                     /locus_tag="ISB30_003909"
     CDS             complement(6256..6996)
                     /gene="pflA"
                     /locus_tag="ISB30_003909"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAP1991493.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(7188..9470)
                     /gene="pflB"
                     /locus_tag="ISB30_003910"
     CDS             complement(7188..9470)
                     /gene="pflB"
                     /locus_tag="ISB30_003910"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP1991494.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(9525..10382)
                     /gene="focA"
                     /locus_tag="ISB30_003911"
     CDS             complement(9525..10382)
                     /gene="focA"
                     /locus_tag="ISB30_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAP1991495.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLIGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         2518 a         2518 c         2748 g         2677 t
ORIGIN      
        1 cgatgtatac aagcctatat agcgatctgc tatagaaata attacacaat acggtttgtt
       61 actggaatca atcgtgagca agcttgagtg agccattatg aaaacgaaaa tccctgatgc
      121 ggtattggct gctgaggtga gtcgccgtgg tttggtaaaa acgacagcga tcggcggcct
      181 ggcaatggcc agcagcgcat taacattacc ttttagtcgg attgcgcacg ctgtcgatag
      241 cgccattcca acaaaatcag acgaaaaggt tatctggagc gcctgtacag ttaactgtgg
      301 tagtcgctgc ccgctacgta tgcacgtcgt ggacggtgaa atcaaatatg ttgaaacgga
      361 taatactggc gatgacaact atgacggcct gcaccaggtg cgcgcctgtc tgcgtggtcg
      421 ttccatgcgt cgccgcgttt acaatcccga ccgcctgaaa tatccgatga aacgagtcgg
      481 ggcgcgcggt gaaggcaaat tcgagcgcat tagctgggaa gaagcctacg acatcatcgc
      541 gaccaatatg cagcgcctga tcaaagagta cggcaacgaa tccatctatc tgaactatgg
      601 caccggtacg ctgggtggca ccatgacccg ctcctggccg ccgggaaata ccctggtcgc
      661 gcgcctgatg aactgctgcg gcggttatct gaaccattac ggtgactact cttccgcgca
      721 aattgcggaa ggtttgaact atacctacgg cggctgggca gatggtaaca gcccgtcgga
      781 tatcgaaaac agtaaactgg tagtgctgtt tggtaataac cctggcgaaa cgcgaatgag
      841 tggcggtggg gtgacttact atcttgaaca ggcgcgccag aaatctaatg ctcgcatgat
      901 catcatcgat ccgcgctata ccgacaccgg tgccgggcgc gaagatgagt ggatccctat
      961 tcgtccggga acagatgccg cactggttaa cggtctggcg tatgtcatga tcaccgaaaa
     1021 cctggtggat caggcattcc tcgataaata ttgcgttggc tacgacgaga aaacactgcc
     1081 agccagtgcg ccgaaaaatg gtcactataa agcttatatt ctgggtgaag ggccagatgg
     1141 cgtggctaaa acgccggaat gggcctcgca aatcactggt gttccggcag acaaaatcat
     1201 caaattggct cgtgaaatcg gtagtaccaa accggcgttt atcagccagg gatggggccc
     1261 gcagcgtcac gctaacggtg aaatcgcaac ccgtgctatc tcgatgctgg cgattctgac
     1321 cggtaacgtt ggtattaacg gaggcaacag cggcgcgcgt gaaggttcat acagcttacc
     1381 gtttgtccgt atgccgaccc tcgaaaaccc gatccagacc agcatttcga tgtttatgtg
     1441 gaccgatgcc attgaacgtg gcccggaaat gacagcgctg cgtgatggtg tgcgcgggaa
     1501 agataagctg gatgtgccga tcaaaatgat ctggaactat gccggtaact gcctgattaa
     1561 ccagcattct gaaatcaacc gtacccatga aatccttcag gatgataaga agtgcgagct
     1621 gattgtggtt atcgactgcc acatgacctc atcggcgaaa tatgctgaca tcctgctgcc
     1681 tgactgcacc gcttcagaac agatggactt tgcactggat gcatcctgcg ggaatatgtc
     1741 ttacgtgatt ttcaacgatc aggtgattaa accgcgcttt gaatgtaaga ccatctatga
     1801 aatgaccagc gaactggcaa aacgtcttgg cgttgagcaa cagtttactg agggccgtac
     1861 ccaggaagag tggatgcggc atctgtatgc ccagtcgcgg gaagcgattc ctgaactgcc
     1921 aacgtttgaa gagttccgta agcaggggat ttttaaaaag cgcgacccac aagggcatca
     1981 cgttgcttat aaagctttcc gtgaagatcc gcaggcaaat ccactgacca cgccatcggg
     2041 gaaaattgag atttattcgc aggcgctggc tgacattgcc gctacctggg aattgccaga
     2101 aggtgatgtg atcgatccac tgccgatcta tacgccgggt tttgaaagtt atcaggatcc
     2161 gctgaacaaa cagtatccgc tgcagcttac gggtttccac tataaatctc gcgttcactc
     2221 aacttacggc aacgttgatg tgctgaaagc agcttgccgt caggaaatgt ggatcaaccc
     2281 gcttgatgcg caaaaacgcg gtatcaacaa cggcgataaa gtcaggatct ttaacgatcg
     2341 tggtgaggtt catattgagg cgaaagtgac gccacgaatg atgccgggtg tggtcgcact
     2401 gggtgaaggt gcctggtatg acccggatac aaaacgtgtc gataagggcg gttgtattaa
     2461 cgtactgacc actcaacgtc cgtctcctct cgctaagggg aatccgtcac atacaaacct
     2521 tgttcaggtt gaaaaggtgt aaggagtaac cgatgacaac ccagtatgga ttttttattg
     2581 attccagccg ttgcaccggt tgcaaaacct gcgagctggc ttgtaaagac tacaaagatt
     2641 tgacgccaga agtcagcttc cgccgcattt atgaatatgc tggtggcgac tggcaggaag
     2701 ataacggtgt ctggcaccag aacgtgtttg cctactatct gtcgatttca tgtaaccact
     2761 gcgaagatcc ggcttgtact aaagtctgcc cgagcggtgc gatgcataaa cgtgaagatg
     2821 gttttgttgt ggtcgatgaa gatgtatgca ttggctgccg ttactgtcat atggcttgcc
     2881 cgtatggcgc accgcaatat aacgaaacga aaggccatat gaccaaatgc gatggttgtt
     2941 atgaccgtgt tgcggaggga aaaaaaccga tctgcgtaga gtcctgcccg ctacgggcgc
     3001 tggatttcgg ccctatcgac gagctgcgta aaaaacatgg cgatctggcg gccgttgcgc
     3061 cgttgccgcg agctcacttt accaaaccga atattgtgat caaacccaat gccaacagcc
     3121 gcccaaccgg ggataccact ggctatctgg caaacccgaa ggaggtgtga gatgggaagt
     3181 ggatggcatg aatggccgct gatgatcttc acggtcttcg ggcaatgtgt cgcgggtggt
     3241 tttatcgttc tggctttggc gctgctcaaa ggcgacctgc gagcagaagc ccagcagcgt
     3301 gttatcgcct gcatgttggg tttatgggtg ctgatgggca ttggctttat ctcctctatg
     3361 cttcatcttg gttcaccgat gcgcgctttt aactcgctca atcgggtagg ggcttcagca
     3421 ctcagtaacg aaatcgccag cggttcgatc ttttttgctg taggcggcat cggctggttg
     3481 ctggcaatgc tgaaaaagct ttcaccggca ttgcgtacgc tttggctggt ggtgacgatg
     3541 gttcttggcg tagtctttgt ctggatgatg gtgcgtgtgt ataacagcat cgatacagta
     3601 cccacctggt acagcatctg gacaccgatg ggcttcttcc tgacgatgtt tatgggcggc
     3661 ccgttactgg gttacctgtt gctgagtctg gctggcgtcg atggctgggc gatgcgtctg
     3721 ctgccagcca tttctgttct ggcactggta gtgagtggcg tgatgtcagt gatgcagggc
     3781 gcagagctgg cgactattca tagctctgtg cagcaggccg cagcgctggt gccggactat
     3841 ggtgcgctga tgtcctggcg gatcgtgctt ttggccgttg ccctgtgcgt gtggattgca
     3901 ccacagctaa aaggttatca gcctgcggtt ccgctacttt ctgtatcatt cattctgctt
     3961 ctggcagggg aattaatcgg tcgtggcgta ttctatggtt tgcatatgac cgtggggatg
     4021 gctgtcgcaa attaatcagt aacaaccggg gtttcggccc cggttcgctt ttatttctgc
     4081 ttcgttaacg tgtcataact ggtcatcaag ttacgataat caggaatgtg gttagagaac
     4141 aacgtcgcca gtccttcaat atcattacgc cagtcgcgat gcagctcgca cgccacgccg
     4201 aaccatgtca tcaattgcgc accagcttgc gacatgcgat cccatgccga atgccgggta
     4261 atttcattaa aggtccctga cgcgtcagtc acgacaaaga cgtcaaaccc ttcttcaatg
     4321 gctgaaagcg ccgggaatgc cacgcaaact tcggttacca caccggcaat aattaactgt
     4381 tttttacctg tcgctttgac agcttttaca aaatcttcgt tatcccaggc gttaatattt
     4441 cccgggcgag caatgtaagg tgcatcgggg aattgtgctt tcagttctgg aactaatggg
     4501 ccgttaggac cggtttcaaa actggtggta agaatagttg gtaagttgaa atacttagcc
     4561 aggtcaccca gcgccagcac gttattttta aacttatcgg gttcgatatc ccgtacaagg
     4621 gaaagtaaac cagcctggtg atcaacaagc aaaacggcag catcattttt atcaagacga
     4681 acatacggtt tggtcatcct cttctccttt cgagagtcag tggtacgtct aaatcggtca
     4741 gacgcgatgc attgctctga aagcatagac gggaaatatg agtttgctgt gaccatgaaa
     4801 tttttcgact gaacgaagcg tatgaaattt gtgttagatc aataaaaaca atcagatgag
     4861 attaatcgtc tcttcagcat tagcgttatg aatgagtaat ataaatcgat aataccgccg
     4921 ctgcgatatg cgcgtatttt cacccattga caatgttttt ggcggtggca tgatgcgcat
     4981 gaaatttgaa cttcctcacg gttttaattc atgtccacgt atacccggcc tgtcatgctt
     5041 ttgctgtctg gcctgctttt gttgactctg gcgatcgcgg tgttaaatac actcgtgccg
     5101 ctttggctcg cccaggaaca catgtctaca tggcaggtag gcgttgtcag ctcatcctat
     5161 tttaccggca accttgtcgg tacattgctg acagggtatg tcattaaacg cattggcttt
     5221 aaccgcagct attatctggc ctccttcatt tttgccgctg gctgtgccgg ccttggcctg
     5281 atgattggat tctggagttg gttggcgtgg cgttttgtcg ctggcatcgg ctgtgccatg
     5341 atttgggtgg ttgttgagag tgcgctgatg tgcagtggga cgtcgcgtaa ccgcgggcgg
     5401 ttgcttgctg cgtatatgat ggtttattac gtcggaacgt ttttaggcca gttactggtc
     5461 agcaaagtct caaccgagtt gatgagtgta ttgccgtggg ttacaggttt gacgttggca
     5521 ggaatcttac cgctgttgtt tacgcgtgtc ctgaatcagc agactgaaaa ccatgattcg
     5581 acgtcaatta cgtcaatgct aaaactccgt caggcgcggc ttggcgtgaa tggctgcatt
     5641 atctcaggaa tcgtgctggg atctctatat ggcctgatgc cgctgtacct caatcacaaa
     5701 ggggtgagca atgccagcat tggtttctgg atggcggtac tggtcagtgc gggtatcctc
     5761 ggacaatggc cgattggacg tctggcggat aagtttggtc gactgctggt gttgcgtgtt
     5821 caggtctttg tcgtcattct cggcagtatt gcgatgctta gccaggcggc gatggcccca
     5881 gcgttattca tcctcggtgc cgctggcttt acgctatatc cggtggcgat ggcctgggct
     5941 tgtgagaaag ttgaacatca tcaactggtg gcgatgaacc aggccttact gttgagctat
     6001 accgtgggaa gcctgcttgg cccgtcattt accgctatgc taatgcagaa tttctccgat
     6061 aatttattgt ttatcatgat cgccagcgta tcgtttatct atttgctgat gctgcttcgc
     6121 aacgccggcc atacgccgaa acccgtggct cacgtgtaaa tgaattcaag cagagtgtga
     6181 acttactgtt tcacactctg cttttctgtg cacaaaaaag cggcaactca ataaagttgc
     6241 cgctttacgg ggaaattaga acatgacctt atgaccgtac tgctcaagaa tgcctttcac
     6301 gcgttccatg gtctctttct tcggtggttt aacaccatcg agtttgtatt cttcacccat
     6361 tgccacccat ttgtgtttgc ccagttcgtg gtaggggagg agctcgattt tctcaacgtt
     6421 gcccatatca cgggtaaatt caccaaggcg atgcgctgaa tcgtcatcgt cagaccagcc
     6481 tgggacaaca acatagcgga tccacacctt cacatttttg ttcgccagat atttagcgaa
     6541 ctccagcgtg cggtggttgg aaactccaac cagattttgg tggatctcgt cgttcatctg
     6601 tttgagatcg agcattacca ggtcggttac ctccagcagt tcatcaatca ccggatcgta
     6661 acgacgaaca aaaccgttgg tgtccagaca ggtatgaatg ccttcttttt tgcaggcgcg
     6721 gaaccagtca cgaacaaact cagcttgtag gattgcctca ccgccggatg cggtaacgcc
     6781 gccgccggaa gcgttcataa agtggcgata ggtcaccact tccttcatca aatcttcaac
     6841 ggtaacttct ttaccgccat gcgtatccca ggtgtcgcgg ttatgacaat acaggcagcg
     6901 catcaggcag ccctggaaaa aggtgataaa gcgaataccc gggccgtcta cggttccaca
     6961 ggattcaaag gagtgaatgc gaccaataac tgacattgcg gtgtttctcc agatgtggcc
     7021 catctgaggc cgtgttggtg cgcagctcga aggctacgtc gagtctgttt tggcagtcac
     7081 cttaaagtat agatagctga caaaaaaggc tctcgtgcta aaaaaggccc cactttcgtg
     7141 gagcctttat tgtacgcttt ttactgtacg atttcagtca aatctaatta catagattga
     7201 gtgaaggtac gagtaataac gtcctgctgc tgttctttag tcagcgagtt gaaacgtact
     7261 gcgtagccag atacacggat ggtcagctgc ggatattttt ccgggttttc catcgcgtcg
     7321 agcagcattt cacggttcat cacgttaacg ttcaggtgct gaccaccttc gatggatgct
     7381 tcgtggtgga agtaaccatc catcagacca gccaggttgg tcttacgaac ttcgtcgtct
     7441 ttacccagtg cgttcggaac gatagagaag gtgtaggaga taccatcttt agcgtaagca
     7501 aacggcagtt tagcaacgga agtcagagac gctacagcac ctttctggtc acgaccgtgc
     7561 atcgggttag cacccggtcc gaacggcgcg ccagcacgac gaccgtctgg ggtgttacca
     7621 gttttcttac catacacaac gttagaagtg atggtcagaa cagactgagt cgggatagcg
     7681 tcacggtagg tgtgcagttt ctgaattttc ttcatgaaac gttctaccag gtcaacagcc
     7741 aggtcatcta cacgcggatc gttgttacca aactgcgggt attcgccttc gatttcgaag
     7801 tcgatagcca gaccgtcttc atcacgaatc ggtttaactt tcgcatattt gattgcagac
     7861 agggagtcag cagcaacgga cagaccagcg ataccacacg ccatggtgcg gataacgtca
     7921 cggtcgtgca gcgccatcag agaggcttcg tagctgtact tgtcgtgcat gtagtggatg
     7981 atgttcagtg cagtgatgta ctgtttagcc agccagtcca tgaagtgatc catgcgctcc
     8041 atcacttcat catagttcag gacatcgcct ttgatcggct cagatttcgg accaacctgc
     8101 attttcagtt tttcgtcaac gccaccgttg attgcgtaca gcatggtttt cgccaggttt
     8161 gcacgcgcac cgaagaactg catttgttta ccaacgatca tcgggcttac gcagcaagca
     8221 atagcgtagt catcgttgtt gaagtccgga cgcatcaggt catcgttctc atactgcaga
     8281 gaagaggtgt cgatggacac tttagcggcg aatttcttga agttcagcgg cagtttttca
     8341 gaccacagaa tggtcatgtt cggttccgga gacggaccca tggtgtacag ggtgttcagg
     8401 aaacggaagc tgtttttggt aaccagggta cgaccgtcga ggcccatacc accgatagat
     8461 tcggttgccc agatcgggtc gccagagaac agttcatcgt attccggagt acgcaggaag
     8521 cgaaccatac gcagtttcat gaccaggtgg tcaaccattt cctgcgcttc ttgttcggtg
     8581 atcttgccag ctttcaggtc acgttcgatg tacacatcca ggaaggtgga ggtacgaccg
     8641 aaggacattg cagcaccgtt ctgagactta acagcagcca ggtagccgaa gtaagtccac
     8701 tggatagctt cctgagcgtt agtagccgga ccagagatgt cgtagccgta tttcgcagcc
     8761 atttctttca tctgacccag agcgcggtgc tgttcagcga tttcttcgcg cagacggata
     8821 gtctgttcca ggtttacgcc gttttccaga tcagcctgca gagaggtgaa ctgtgcaagt
     8881 ttgtctttca tcaggtagtc gataccgtac agtgcaacgc gacggtagtc accgatgata
     8941 cgaccacggc cataagcatc tggcagaccg gtcagaacac cggatttacg gcaacgcagg
     9001 atgtccggag tgtaaacgtc gaacacgccc tggttgtgag ttttacggta ttcagtgaag
     9061 atttttttga tcatcgggtc cagttcgcgg ttgtacgctt tgcaggaacc ttcgatcatt
     9121 ttgataccac cgaacgggat aagagcacgt ttcagcggag cttcagtctg cagaccaacg
     9181 attttctcaa gctgcttgtt gatgtaacca gcgtcgtgag aggtgatggt ggaagcaaca
     9241 gcggtgtcaa agtcaactgg cgcgtgagtg cggttttcca gtttaacgcc ttccattact
     9301 ttgtcccaca gggtggtggt cgcttcagta gcgccagcca ggaaggactc gtcaccctcg
     9361 tacggagtgt agtttttctg aatgaagtca cggacgttta cttcattctg ccagtcacct
     9421 ttggtaaaac cttcccaggc tgtggctaac ttttcattaa gctcggacat gtaacaccta
     9481 ccttcttaag tggatttttt atttactgcg tacttcgaca atcattaatg gtgatcgttt
     9541 tcacgcaggt aaatgaccca gtatgtcaac ccaaccaaca aaccaccgcc gataatatta
     9601 ccgatcgtaa ccggaatcag gttatcagtg atgaagttca tcacggtcag gtgagaaaaa
     9661 ttttccggtg cagaaccgac agcggtccag aattccggag atgcgaagtc gcggattaca
     9721 atacccatcg ggatcataaa catgtttgcg atactgtgct caaaaccgct ggcaacaaac
     9781 atcgcgaccg gcagcaccat aatgaacgct ttgtccatca ggctgcggcc agaataactc
     9841 atccatactg ccagacatac catcaggttt gccaggatac caagacagac ggcctcaata
     9901 aaagtatggt gcactttgtg gtcggcggtt tgtaggacgt ttagtcccca ttgaccattt
     9961 gcggtcatat actcgccgga aagccacatt aaaagtacaa acagcagtgc gccgaccagg
    10021 ttgccaaaat aaacatttag ccagtttttc gccaactgac cccaggtgat gcgcccactc
    10081 gccttggcaa caacaatcaa cactgtggaa gtaaagagat cggctccgca gacaacacaa
    10141 agaatcaacc ccagagagaa gcaaatgccg ccaatcagtt ttgccatgcc gaagggcatt
    10201 gtacctgtgc cagtggttgc tgtgatatag aagacgaatg cgattgagat gaaaacaccg
    10261 gcggtaatcg ccagatagaa agtcttaagc ggatgtttcg ttgctttata gacacctgcc
    10321 tcttcggcca ctttggccat tgcagcagga agtaaaagat caaaagggtt gtcagctttc
    10381 acactaactc tctctttatt aagtcggcga cgagatacta acaaagcatt atagatgaga
    10441 aattgatata gatcatatct c
//
LOCUS       DACADF010000151        10422 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.153, whole genome shotgun sequence.
ACCESSION   DACADF010000151 DACADF010000000
VERSION     DACADF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.153"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            84..2225
                     /locus_tag="ISB30_003912"
     CDS             84..2225
                     /locus_tag="ISB30_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HAP1991496.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2289..4181)
                     /gene="parE"
                     /locus_tag="ISB30_003913"
     CDS             complement(2289..4181)
                     /gene="parE"
                     /locus_tag="ISB30_003913"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP1991497.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRLDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4210..4791)
                     /gene="yqiA"
                     /locus_tag="ISB30_003914"
     CDS             complement(4210..4791)
                     /gene="yqiA"
                     /locus_tag="ISB30_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAP1991498.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4791..5618)
                     /gene="cpdA"
                     /locus_tag="ISB30_003915"
     CDS             complement(4791..5618)
                     /gene="cpdA"
                     /locus_tag="ISB30_003915"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAP1991499.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(5643..6065)
                     /locus_tag="ISB30_003916"
     CDS             complement(5643..6065)
                     /locus_tag="ISB30_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAP1991500.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6066..6695)
                     /gene="nudF"
                     /locus_tag="ISB30_003917"
     CDS             complement(6066..6695)
                     /gene="nudF"
                     /locus_tag="ISB30_003917"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAP1991501.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6900..8381
                     /gene="tolC"
                     /locus_tag="ISB30_003918"
     CDS             6900..8381
                     /gene="tolC"
                     /locus_tag="ISB30_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAP1991502.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8529..9200
                     /locus_tag="ISB30_003919"
     CDS             8529..9200
                     /locus_tag="ISB30_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAP1991503.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9206..10366
                     /locus_tag="ISB30_003920"
     CDS             9206..10366
                     /locus_tag="ISB30_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAP1991504.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLSQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         2623 a         2772 c         2583 g         2444 t
ORIGIN      
        1 ataacaaacg gatagtgctt agtgagcaat aagaaacaat tttcactcgc gacaatccgc
       61 gagtttttgg gataacagac taaatggcta tgttcacacc ttcattctca ggactcaaag
      121 gtcgggcgct cttttcactg ctttttgcgg caccgatgat tcatgcaaca gactctgtaa
      181 cgaccaaaga tggcgaaaca atcactgtta cagcagatgc aaataccgca actgaggcaa
      241 ccgatggtta tcaacctctg agcacctcta cggcgacatt aaccgatatg ccgatgctgg
      301 atatcccgca ggtggtcaat acggttagcg atcaggttct ggaaaaccag aatgcgacaa
      361 cgctggatga ggcgctttat aacgtcagta acgtggtaca gaccaataca ttaggcggaa
      421 ctcaggacgc ctttgtacgc cgtgggtttg gcgcaaaccg ggatggctcc atcatgacca
      481 acggtctgcg aaccgtactt cctcgcagtt tcaacgccgc cacagaacgt gtggaagtgc
      541 taaaaggccc ggcctccacg ctgtatggca ttctcgatcc tggcggactg attaacgtcg
      601 tgaccaagcg cccggaaaaa acattccatg gttcggtttc agccacctcc tccagttttg
      661 gtggcggcac tgggcaactt gatatcacag gtcccattga aggcactcag ctggcgtatc
      721 gccttaccgg ggaagtgcag gatgaagatt actggcgaaa cttcggtaaa gagcgcagta
      781 catttattgc cccgtcactc acatggtttg gtgataatgc aacagtaacc atgctctatt
      841 cccatcggga ctataaaact ccgttcgatc gtggaacgat tttcgacctt acgacgaaac
      901 agcccgtaaa cgttgatcga aaaatacgtt ttgacgaacc gtttaatatt acagatggtc
      961 agtccgatct ggcgcaactc aacgcagaat atcatctcaa tagccagtgg acagcacgct
     1021 ttgattacag ctacagccag gataaataca gcgataatca ggcgcgtgtt accgcatatg
     1081 atgcaacgac aggaacactg acacggcgtg ttgatgcaac tcagggctct acccagcgta
     1141 tgcatgctac tcgtgcggat ctgcaaggga atgttgatat tgccggattc tataatgaga
     1201 ttctgggtgg ggtgtcatat gaatattatg atcttctgcg tacagatatg attcgctgta
     1261 aaaaagctaa agatttcaat atttacaacc ctgtttatgg taataccagc aaatgtacaa
     1321 cggtttcggc gtcggacagt gatcagacga tcaaacagga gagctactca gcgtatgcac
     1381 aggacgcact ctatctgacc gataactgga ttgccgtcgc cgggatccgc tatcagtatt
     1441 acacgcagta tgcgggtaaa ggccgtcctt ttaatgtcaa tactgacagc cgcgatgaac
     1501 aatggactcc caaactgggg ttggtctaca aactgacgcc atcggtatcc ttatttgcca
     1561 attattcgca aacatttatg ccgcagtcgt caattgccag ctacattggc gatcttccac
     1621 cggaatcatc taatgcttac gaagtcgggg caaaattcga gctattcgat ggtatcaccg
     1681 cagatattgc gctgtttgat atccataaac gtaatgtgtt gtataccgaa agtattggtg
     1741 atgaaaccat cgccaaaacg gcaggccgtg ttcgttcaag aggggtagaa gtcgaccttg
     1801 cgggagcatt aactgaaaac attaatatca ttgccagcta cggctatacc gatgctaagg
     1861 ttctggagga tcctgattat gcagggaaac cattgccgaa tgttcctcgt cataccggtt
     1921 cgctattcct gacctatgat attcataaca tgccaggcaa taatacgctg acatttggcg
     1981 gtggtggaca tggtgtaagc cgtcgttcgg caaccaatgg ggctgactat tatctgccag
     2041 gctattttgt tgccgatgcc ttcgccgcat acaaaatgaa attgcagtat ccggtcacac
     2101 tgcaattaaa cgtcaaaaac ctgtttgata aaacgtatta cacctcttcc atcgccacaa
     2161 ataatctggg gaatcagatt ggcgatccgc gtgaagtgca attcacggtg aaaatggaat
     2221 tttgatcaaa aaagcccggt atacacttgt ataccgggct atgttttatt cttctggcgg
     2281 gaggattatc agacctcaat ctctgccatg tcgccttttt cttgcaacca gttgcggcga
     2341 tcttccgagc gtttcttcgc cagcagcata tccatcatcg cgtcagtacg ctgatcgtct
     2401 tcatcatcga tagtcaactg caccagacgg cgagtgttcg gatcaagcgt ggtttcgcgc
     2461 aattgcatcg ggttcatttc cccgagacct ttaaaacgct ggacgtttgg cttgcctttc
     2521 ttgcgtttta attgctcaag tacgccctct ttctcttctt ccgtcagcgc gtaataaacc
     2581 tctttcccga ggtcaagacg gtagagcggt ggcagtgcaa cgtaaacgtg accgtgtttc
     2641 accaatgcgc ggaagtgttt tacaaacaaa gcgcagagca gcgtggcaat gtgcagacca
     2701 tcggagtcag catccgccag gatacagatc ttgccgtaac gaagctggct caaatcgtcg
     2761 ctgtcaggat cgataccgat agctaccgaa atatcgtgca cttcctgcga agccagcact
     2821 tcgtcggaag agacttccca ggtgttaagg atcttacctt tcagtggcat gatcgcctga
     2881 tattcgcgat cgcgcgcctg cttggcagat ccgcctgcgg agtcaccttc cacaaggaat
     2941 agttcggtac ggttaagatc ctgcgcggta cagtcagcca atttaccagg cagcgccggg
     3001 ccgctggtta gctttttacg caccactttt ttagccgcac gcatacgacg ctgggcgctg
     3061 gaaatcgcca tctctgccaa cagctccgcc gcctgaacgt tttggttcag ccacaggatg
     3121 aaggcatctt tcaccacgcc cgaaacgaat gccgcacact gacgcgaaga gagacgttct
     3181 ttggtctgcc cggcaaactg cggatcctgc atttttactg acagcacata ggcgcagcga
     3241 tcccagatat cttccgccga cagctttaca ccacgcggca gaatgttgcg gtattcacag
     3301 aactcacgca tcgcgtccag caggccctga cgcaggccat taacatgggt accgccctgc
     3361 atcgttggga tcaggttgac gtagctttcg gtcagcagtt caccgccttc cggcagccac
     3421 agtagcgccc agtccaccgc ttcagtatcg ccagcgaaat taccgataaa cggtttttct
     3481 ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat cattcagacc gtcctgatag
     3541 caccagcgct gttcggtgtt gttgatctca tctttaaaag tgatctcaac gcccgggcac
     3601 agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa cagaaaaacg cgggctgtca
     3661 aagaaggttt catccggcca gaagtgcacg ctggtaccgg tattgcgttt accgcaagtg
     3721 ccgacaacct gtaaatcttg caccttttcg ccattttcaa aggcgatgtt atagatctga
     3781 ccatcgcggc gtacgttaac ttctacgcgc ttcgacaggg cgttaactac cgaaatcccc
     3841 acgccatgca ggccgccaga gaactggtaa tttttgttag agaatttacc gcccgcgtgc
     3901 agacggcaaa gaatcagttc aaccgccggt accccctctt ccgggtgaat atctaccggc
     3961 atcccgcgcc catcgtcaat cacttctaac gactggtcgg catgtaagat tacatccacg
     4021 cgttttgcgt ggcccgccag tgcttcatcg acactgttat caataacttc ttgccccaaa
     4081 tggttagggc gagtggtatc ggtatacatc cccggacggc ggcgaaccgg ctcaagcccg
     4141 gtgagtacct caatggcatc agcgttataa gtttgcgtca tggtttaagt tagcaattcg
     4201 agttgatcgt cagagatggt gcagaccaag aaaatcgacg atcgggttga aataatcttc
     4261 gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc tggcggcagg aagcgtagta
     4321 cgccaccgcc tggcggtaat ccagcacttc atctcccgtc tgttgcagca gccagatcaa
     4381 atccggcgct tccagcgggt caatctgcat gactttgaga tcgtaaatat ggcgtgactc
     4441 tagcacatat tgctgcccgg tgtaggggtt ctcgttctga ccgagatagt ccgtcagcag
     4501 ttcaaacggg cgcaccgccg ggttgaccac cactgcgggc agcataaaac attgcgacaa
     4561 ccaggtggcg taatatcccc ccagtgacga accgacaata cccagcgaat cgccgccatg
     4621 ttccaggaca atggattcca gcagttctgc cgcgtcggaa ggatacggcg gcagctgcgg
     4681 aatgatcatc tcaacgtcag ggtgatgttc cgccagccag ttttttaaca agctcgcttt
     4741 tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca tcagtagcct
     4801 tctgaagcgg tatcaggttg gaagcgtgcg tccgccaggc gatgcacctc ggtggtcagc
     4861 gtgccatcag catgtaactc aagagtacgc cagccgggcg cgatggtatc cagcgtaaag
     4921 ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg ttgccagcag gcggcgaccg
     4981 ttccagtcga gatccagctc ctgatgaata tgaccgcaca gcaagtattt gacgtgcgga
     5041 aacttcgcca gcacggtatc cagttcgccc gcgttacgca ggctgtgttg atcaagccaa
     5101 ctacaacccg caggtagcgg atgatgatgc agcagcagca acgtatggcg ttctggcgca
     5161 tcggccagtt tacgttccag ccactcaagc tgaaactcgc tcagctcacc gtgcggcacg
     5221 ccaaacacct ggctatccag caacaggatt tgccattgct caccaataaa cacccgcttc
     5281 gccggggaga tacccgcatc ctgtaacgca ctgtacatcg cgggctggaa atcgtggttg
     5341 cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa atgctgatag
     5401 gccgcagagg attgatcctg cgctaaatca cctgtcgcga caatcaggtc gaattcgtgc
     5461 tggtgtggcc gaatcgcctc cagcaccgcc tggtagcttt cccaggtgtt tacccctaac
     5521 agggcttcgt gcttttgtgc aaacaggtga gtgtcggtaa tttgtaaaat cctgactctg
     5581 gcctcaccag ccagaggaag ggttaacagg ctttccaaat ggtgtcctta ggtttcacga
     5641 cgctaataaa ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa ccagtccgct
     5701 aaaaactgat taatttgatg cttttcgtcg cgttgatgca actttttatt aggataatca
     5761 taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca cttcagccac catcgcgtca
     5821 tgatacagac gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg cgcagtctgt
     5881 tctattgtca ccagggtagt gtatcgggtc gattccacaa tcgtcagccg atattgtgcg
     5941 tttgccacct gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa caaacggcgc
     6001 aattgtgaaa agttcatctc gcacaggcgc atcatttcag gaaagtcagg tgtgtaacgc
     6061 ttcatttatg cccactcatt ttttaacgct tgatgatgca gctgcagcca ttgcaaagcg
     6121 atgaccgacg ctgcgttgtc gattttcccc tcttctaccc actggtatgc ctgttcccgg
     6181 cttaccacat gaacgcgaat atcttcgttt tcatcagcca gaccgtgaat accgcttgcg
     6241 gtcgtggcgt ccacttcgcc caccataatt gacgaacgct cactggtgcc ccccgggctt
     6301 gccaggaaac ttaacaccgg tttggtccgt ttgactatca gtcccgcctc ttcaatcgct
     6361 tcgcgacggg caacatcttc cacactttca ccctcttcaa tcatcccggc aaccatctcc
     6421 agtagccaag gggtttcgct ggtgtcgtac gcggcaatcc gaatctgctc aatcagcaca
     6481 acttcatcac gcactgggtc aaagggtagc aagactgcgg cgtgaccgcg ctcaaaaatt
     6541 tcccgccgta cctcatgact catttgcccg ttgaatagac gatgacgaaa tctataaaga
     6601 tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac atcgtttttg
     6661 ccaaatgtaa cgggcaggtt gtctggctta agcattgtta atgtcctggc actaatagtg
     6721 aattaaatgt gaatttcagc gacgtttgac tgccgtttga gcagtcatgt gttaaattga
     6781 ggcacattaa cgccctatgg cacgtaacgc caaccttttg cggtagcggc ttctgctaga
     6841 atccgcaata attttacagt ttgatcgcgc taaatactgc ttcaccacaa ggaatgcaaa
     6901 tgaagaaatt gctccccatt cttatcggcc tgagcctctc tgggttcagt tcgttgagcc
     6961 aggccgagaa cctgatgcaa gtttatcagc aagcacgcct tagtaacccg gaattgcgta
     7021 agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc agtccattac
     7081 tgccacagct aggtttaggt gcagattaca cctatagcaa cggctaccgc gacgcgaacg
     7141 gcatcaactc taacgcgacc agtgcgtccc tgcagttaac tcaatccatt tttgatatgt
     7201 cgaaatggcg tgcgttaacg ctgcaggaaa aagcagctgg gattcaggac gtcacgtatc
     7261 agaccgatca acaaaccttg atcctcaaca ccgcgaccgc ttatttcaac gtgttgaatg
     7321 cgattgacgt tctttcctat acacaggcgc aaaaagaagc gatctaccgt caattagatc
     7381 aaaccaccca acgttttaac gtgggcctgg tagcgatcac cgacgtgcag aacgcccgcg
     7441 cacagtacga taccgtgctg gcgaacgaag tgaccgcacg taataacctt gataacgcgg
     7501 tagagcagct gcgccagatc accggtaact actatccgga actggcggcg ctgaatgtcg
     7561 aaaactttaa aaccgacaaa ccacagccgg ttaacacgct gctgaaagaa gccgaaaaac
     7621 gcaacctgtc cctgttacag gcgcgtttga gccaggacct ggcgcgcgag caaattcgcc
     7681 aggcgcagga tggtcactta ccgactctgg atttaacggc ttctaccggg atttctgaca
     7741 cctcttatag cggttcgaaa acccgtggtg ccgctggtac ccagtatgac gatagcaata
     7801 tgggccagaa caaagttggc ctgagcttct cgctgccgat ttatcagggc ggaatggtta
     7861 actcgcaggt gaaacaggcc cagtacaact ttgttggtgc cagcgagcaa ctggaaagcg
     7921 cgcatcgtag cgtcgtacaa accgtacgtt cctccttcaa caacattaat gcttctatca
     7981 gtagcattaa cgcctacaaa caagccgtag tttccgctca aagctcatta gacgcgatgg
     8041 aggcgggtta ctcggtcggt acgcgtacca ttgttgatgt gttggatgcg accaccacgc
     8101 tgtacaacgc taagcaagag ctggcaaatg cgcgttataa ctacctgatt aaccagttga
     8161 atattaagtc agctctgggt acgttgaacg agcaggatct gctggcactg aacaatgcgc
     8221 tgagcaaacc ggtttccact aatccggaaa acgttgcccc gcaaacgccg gaacagaatg
     8281 ctattgctga tggttatgcg cctgatagcc cggcaccagt cgttcagcaa acatccgcac
     8341 gcactaccac cagtaacggt cataaccctt tccgtaactg acgacgacga cggggcttcg
     8401 gccccgtctg aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt cttccccctt
     8461 ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg tatttaccac tggtcccgga
     8521 agacaaaaat gaaacggaca aaatccatac gccacgcatc gttccgcaaa aactggagcg
     8581 cacgccatct gacaccagtc gctctcgcgg ttgccactgt ttttatgctg gcgggctgtg
     8641 aaaagagtga tgaaacagtg tctctctatc aaaatgctga cgactgttca gctgcaaacc
     8701 caggcaaaag cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa gccgaacgta
     8761 ctgcgccgaa atacgccacc cgtgaagact gtgttgctga atttggtgaa ggtcagtgcc
     8821 agcaggcacc agcccaggct ggcatggcac cagaaaacca ggcgcaggcc cagcaatcca
     8881 gcgggagttt ctggatgccg ctgatggccg gttacatgat gggacgtctg atgggcggcg
     8941 gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa cccggccagc ccggcttacg
     9001 gtaaatatac cgacgcgacc ggtaaaaact atggcgcagc ccagccaggc cgcaccatga
     9061 ccgtaccgaa gacggcaatg gcaccaaaac cggcgaccac tactactgtt acccgtggcg
     9121 gttttggtga atctgttgcc aaacaaagca ctatgcagcg tagcgcaacc ggcacctctt
     9181 ctcgttcaat gggtggctga taccgatgga aagagtcagt attaccgagc gcccggactg
     9241 gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc atgtacggcg agccgtactg
     9301 gtgtgaagat gcttactaca agttgaccct ctctcaggtt gaaaagctgg aagaagtcac
     9361 cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca gcgatgagct
     9421 gatgaccaaa ttccgcatac caaaacacac ctggagtttt gtgcgccagt catggctgac
     9481 gcaccagcca tcgctttatt cgcgtcttga tctggcgtgg gatggcactg gcgaacctaa
     9541 acttctggaa aataacgccg atacgccaac gtcactatac gaggcggcgt tctttcagtg
     9601 gatctggctg gaagatcagc ttaacgccgg taacttgccg gagggcagcg accagtttaa
     9661 cagtctgcaa gaaaagctga tcgatcgctt cgttgagctg cgtgaacagt atggcttcca
     9721 gttgctgcat ctcacctgct gtcgcgacac ggtggaagat cgcggaacca ttcagtattt
     9781 gcaggactgc gcaacggaag ctgaaatcgc caccgagttc ctctacattg acgatatcgg
     9841 gttaggtgaa aaaggtcagt tcacggattt acaggatcag gtgatttcca acctgttcaa
     9901 gctgtatccg tgggaattta tgttgcgtga gatgttctcc accaagctgg aggatgcagg
     9961 cgtacgctgg ctggaaccgg cgtggaagag cattatctcc aacaaagcgc ttctaccgct
    10021 actgtgggag atgttcccga atcacccgaa cctgctgccc gcttattttg cggaagatga
    10081 tcatccgcaa atggaaaaat atgtggttaa accgatcttc tcccgtgaag gcgcaaacgt
    10141 gtcgatcatt gagaacggca aaaccattga agcagcggaa ggtccgtatg gcgaagaagg
    10201 gatgattgtt cagcaattcc acccgttacc gaaattcggc gacagctata tgctgattgg
    10261 tagctggctg gtgaacgatc aacctgccgg aattggcatt cgtgaagacc gtgcattgat
    10321 cacccaggat atgtctcggt tttatccaca tatttttgtt gagtaataca gcttaatatt
    10381 gccccgataa gcggggcaat ctcttataat ccaccattca ta
//
LOCUS       DACADF010000152        10205 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.154, whole genome shotgun sequence.
ACCESSION   DACADF010000152 DACADF010000000
VERSION     DACADF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.154"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            127..1521
                     /locus_tag="ISB30_003921"
     CDS             127..1521
                     /locus_tag="ISB30_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP1991505.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1598..2095
                     /locus_tag="ISB30_003922"
     CDS             1598..2095
                     /locus_tag="ISB30_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAP1991506.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLIAK"
     gene            2190..2897
                     /gene="endA"
                     /locus_tag="ISB30_003923"
     CDS             2190..2897
                     /gene="endA"
                     /locus_tag="ISB30_003923"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAP1991507.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2977..3708
                     /gene="rsmE"
                     /locus_tag="ISB30_003924"
     CDS             2977..3708
                     /gene="rsmE"
                     /locus_tag="ISB30_003924"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP1991508.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3721..4671
                     /gene="gshB"
                     /locus_tag="ISB30_003925"
     CDS             3721..4671
                     /gene="gshB"
                     /locus_tag="ISB30_003925"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAP1991509.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQVGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4708..5343
                     /locus_tag="ISB30_003926"
     CDS             4708..5343
                     /locus_tag="ISB30_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAP1991510.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5343..5759
                     /gene="ruvX"
                     /locus_tag="ISB30_003927"
     CDS             5343..5759
                     /gene="ruvX"
                     /locus_tag="ISB30_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP1991511.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5874..6854)
                     /locus_tag="ISB30_003928"
     CDS             complement(5874..6854)
                     /locus_tag="ISB30_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAP1991512.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRTILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            6872..7576
                     /gene="yggS"
                     /locus_tag="ISB30_003929"
     CDS             6872..7576
                     /gene="yggS"
                     /locus_tag="ISB30_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAP1991513.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7594..8160
                     /gene="yggT"
                     /locus_tag="ISB30_003930"
     CDS             7594..8160
                     /gene="yggT"
                     /locus_tag="ISB30_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAP1991514.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8157..8447
                     /locus_tag="ISB30_003931"
     CDS             8157..8447
                     /locus_tag="ISB30_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAP1991515.1"
                     /translation="MSAVTVNDGGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8455..9048
                     /locus_tag="ISB30_003932"
     CDS             8455..9048
                     /locus_tag="ISB30_003932"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAP1991516.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9041..10177
                     /gene="hemW"
                     /locus_tag="ISB30_003933"
     CDS             9041..10177
                     /gene="hemW"
                     /locus_tag="ISB30_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAP1991517.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIQYTGLS
                     EEVIRSQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2400 a         2691 c         2698 g         2416 t
ORIGIN      
        1 acttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
       61 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac catattggag
      121 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
      181 tgcttccttg ctgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
      241 gcactgccgt ttattgcaga tgaattccag attacttcgc acacgcaaga atgggtcgta
      301 agctccatga tgttcggtgc ggcagtcggt gcggtgggca gcggctggct ctcctttaaa
      361 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
      421 tctgcggctg cgccaaacgt tgaagtactg attctttccc gcgttctgct ggggctggcg
      481 gtgggtgtgg cttcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
      541 cgcggcagta tgatctcgat gtatcagttg atgatcacta ttgggatcct cggtgcttat
      601 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
      661 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
      721 gctgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tgcgcctgcg tgacaccagc
      781 gcggaagcga aacgtgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
      841 tgggcgctgt ttaaagagaa cagcaacttc cgccgcgcgg tgttccttgg cgtactgttg
      901 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
      961 gaactggcgg gttataccaa caccaccgag caaatgtggg ggacggtgat tgtcggcctg
     1021 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     1081 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     1141 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     1201 attgttggct ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     1261 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     1321 atgatcgttg gcgcaacctt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     1381 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     1441 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     1501 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtcgcggagg aagccacctc
     1561 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     1621 cgccatccaa caggccgtta tgcgtcgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     1681 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     1741 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     1801 tgaagccttt attgaagaag tggtgccgca cgaactggca catttgctgg tatggaaaca
     1861 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     1921 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     1981 ctaccgctgc aagtgccagg agcatcagct taccgtacgc cgccataatc gcgtagttcg
     2041 tggcgaggcc gtctatcgct gtgttcactg cggtgaacag ctgattgcaa agtaaccacc
     2101 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     2161 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     2221 tactgagcgc agcattttcc ggcccggcgt tggccgaagg tatcaatagt ttttctcagg
     2281 cgaaagccgc ggcggtaaaa gtccacgctg acgcgcccgg tacgttttat tgcggatgta
     2341 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     2401 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     2461 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     2521 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     2581 gcgatcgcgg caactttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     2641 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     2701 ccattgcgcg cacctacttc tatatgcgtg accaatacaa cctgacactc tctcgccagc
     2761 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     2821 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     2881 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     2941 aacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     3001 cctgaaccac tgaccagcca ttctcatatc gcgctttgcg aagatgctgc caaccatatc
     3061 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg cagcaaccag
     3121 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgttggaa
     3181 ggacagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     3241 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     3301 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctc
     3361 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     3421 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     3481 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgccgttacc ggttgaacgc
     3541 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     3601 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     3661 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     3721 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     3781 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     3841 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     3901 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     3961 gacgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     4021 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     4081 gactgtaacg agaaactgtt tactgcctgg ttctctgact taacgccaga aacgctggtt
     4141 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     4201 ccactggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     4261 ggcgtgattg ccgaaaccct gactgagcat ggcactcgct actgcatggc gcaaaattac
     4321 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tagatggcga gccggtaccg
     4381 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     4441 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca ggtcgggccg
     4501 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     4561 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     4621 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     4681 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     4741 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     4801 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     4861 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     4921 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     4981 ctggataaac cggttatgct cggcggccct ctggctgaag atcgcgggtt tattttgcat
     5041 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     5101 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     5161 ctgggctatg cctcctggga gaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     5221 ctaacggccc cggcggatct gaatattctg ttcaaaacac cgattgccga ccgctggcgc
     5281 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc agggcacgcc
     5341 tgatgagtgg aaccttactc gccttcgact tcggtaccaa aagcattggt gtagcggtcg
     5401 gccaacgcat taccggcacc gctcgcccat tgcctgcaat taaagcacag gacggtacgc
     5461 cggactggaa cctcatcgaa cgtttactga aagagtggca gccggacgaa atcatcgtcg
     5521 gtttgccgct gaatatggac ggcaccgagc aaccattgac tgccagagcg cgtaaatttg
     5581 ccaaccgtat tcatggccgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     5641 ccgtggaggc acgttccggt ctgtttgaac agggcggcta tcgggcgctc aacaaaggca
     5701 aagttgactc tgcctctgcg gttatcattc tcgaaagtta tttcgagcag ggatattaag
     5761 gcgatttacg tcatcgctcc ttattgccgg atgcggcgtg aatgactacg tttgtaggcc
     5821 tgataagcgt agcgcatcag gcatatttac taaaataatc aatcacttta gcctcacaaa
     5881 cgcccttccc ccacccgctg ctgataactc tgctgaaacg ttaacatccc cacctgctgc
     5941 ccggtttgaa taacatgcgg taactggtgg gttttccctt cgcgaatcaa attccccacc
     6001 gcgggagtgt taatcagtaa ttcaaacagc gccacgcgtc cttcctgttt atccacttcc
     6061 agcttttgtg acaacactgc ccgtaaactc cccgccagtt gattacgtac gggatctttt
     6121 tcctgcgccg gaaatgaatc caccagccgc tcaactgcct gcgccgcacc acgcgtatgt
     6181 aatgttgcca gcaccaaatg cccggtttct gccgccgtca gtgccagacg gattgtctcg
     6241 ctgtcacgca gctctccgag caatatcaca tcgggatctt cccgcaatgc ggcacgcaat
     6301 cccgatgcga acgtcataca gtgcaaacca atttcccgct gctggatcaa acatcgctgg
     6361 ctggcataga gatattcaac aggatcttcc agcgtcagaa tatgcgcatc ggcatgttga
     6421 ttgagatagc caaccatcgc cgccagcgtg gtagatttgc cgctccccgt cgcccccgtc
     6481 accagaatca ggccattctc gctcttgagt aattccggca ataccggtgg cgcaccaagc
     6541 tgttcgagct gtggacagtg cgaaggtaac aaccgtaatg ccagagaaat accttgccgt
     6601 tgcgcgaacg cactgccacg caaccgctgg ttttccgcca gcgacacagc aaaatccaac
     6661 tgaccattct ccagcaatat tgtccgctga tcgtcatcca gccactcccg cagtagctct
     6721 tcgacgtccg gcgcatcaaa cggcgcagct tccattcgcc cgcgaatacg ccatcgtgcg
     6781 ggccaggcgc tgcacaggtg tagatccgag acgttatgct ttacactaag ggccacaatt
     6841 tcttccatat tcatactaag atcctcggaa aatgaacgat attgcgcata acctggcaca
     6901 ggtccgggac aaaatctcag cggctgcaac gcgttgcggc cgttctccag aagaaattac
     6961 gctgcttgca gtcagtaaaa caaaacctgc gagcgccatc gcagaagcca ttgatgccgg
     7021 gcagcgtcaa tttggtgaaa actacgttca ggaaggggta gataaaattc gccactttca
     7081 ggaactgggc gtaacaggat tagaatggca ttttattggc ccgttgcagt ctaataaaag
     7141 ccgcctggtg gcagagcatt tcgactggtg tcataccatc gaccgtttgc gcattgctac
     7201 ccgcctcaac gatcagcgcc cggcagaact tccccctctt aacgtactga ttcaaattaa
     7261 tattagtgac gaaaacagta agtccgggat tcaactggca gaactggacg agctggcagc
     7321 tgcggtcgct gaactaccgc gtttacgtct gcgcgggctg atggcaatcc ctgcacctga
     7381 gtcagaatat gtaaggcagt ttgaagttgc acgccaaatg gctgtagcat ttgccggact
     7441 gaaaacgcgc tacccgcata tcgacacgct ctctctggga atgtcggacg atatggaagc
     7501 cgccattgcg gcaggtagca cgatggttcg tatcggcact gcaatttttg gtgcgcgtga
     7561 ttactctaaa aaataaggaa ttaaaggaac gccatgaata cgttgacttt cctgctttca
     7621 acggtcattg agctgtatac catggtgctg ttattacgca tctggatgca gtgggctcat
     7681 tgtgattttt acaacccctt ctcacagttt gtagtgaagg taacgcagcc aattatcggg
     7741 ccactgcgcc gcgttattcc ggcaatgggg cccattgaca gcgcctcact gctggttgcg
     7801 tacattctca gtttcatcaa agccatcgtg ctgtttaaag tggtgacctt cctgccaatc
     7861 atctggattg ccggtttact gattctgctg aaaaccatcg gcctgctgat tttctgggtc
     7921 ctgctggtga tggcgattat gagctgggta agccaggggc gtagcccgat tgaatacgtg
     7981 ctgattcagc tggccgatcc gctgctgcgc ccgattcgcc gcctgctacc ggcaatgggt
     8041 gggattgatt tctcgccgat gatcctcgtt ctgctgctgt atgtcatcaa tatgggtgtc
     8101 gcagaagtct tgcaggcgac cggaaatatg ctgctgccgg ggctgtggat ggcgttatga
     8161 gtgccgtaac agtgaatgat ggcggtctgg ttttacggct ctatattcag ccgaaagcca
     8221 gccgtgattc tattgtcggt ttacatggcg acgaggtaaa agtcgccatt accgcgccgc
     8281 cggttgacgg ccaggccaac agccatctgg tgaagtttct cggtaagcaa ttccgggttg
     8341 ccaaaagcca ggtggtgatt gaaaaaggcg aacttggccg ccacaaacaa attaaaatca
     8401 ttaatccgca acaaatcccg ccagaaatcg cggcgttaat taattaggta tcctatgcaa
     8461 aaagttgtcc tcgcaaccgg caatgccggt aaagtgcgtg agctggcgtc gctgcttagc
     8521 gacttcggtc ttgatatcgt ggcccaaaca gacctcggtg tagattccgc tgaagaaact
     8581 ggcctgacct ttatcgaaaa cgcgattctg aaagcgcgcc atgcggcaaa agtgaccggt
     8641 ttaccggcaa ttgccgacga ctccggtctg gcggtagatg tacttggcgg tgcaccgggg
     8701 atttactccg cgcgttattc cggtgaagac gcgaccgatc aaaagaatct gcaaaaactg
     8761 ctggaaacaa tgaaagacgt accggacgac caacgtcagg cgcgtttcca ctgcgtgctg
     8821 gtatatctgc gtcacgcgga agatcccact ccgctggtgt gccacggtag ctggccgggc
     8881 gtgattactc gtgaaccagc gggcactggt ggctttggtt atgatccaat cttctttgtt
     8941 ccttccgaag ggaaaaccgc tgccgaactg acccgcgaag aaaagagcgc catttcccac
     9001 cgtggtcagg cattgaaact gctgctggac gctttacgta atggttaaat taccgccgct
     9061 gagtctctac attcacatcc cgtggtgcgt gcagaaatgc ccgtactgcg atttcaactc
     9121 tcacgcgttg aaaggagaag tgccgcacga cgattatgtt cagcatctgc ttaacgatct
     9181 ggacaacgat gtggcttacg ctcagggccg tgaagtgcag acaatcttta ttggcggtgg
     9241 tacgccgagc ctgctttccg gcccggcgat gcaaacgctg ctggacggcg tgcgtgcgcg
     9301 tttgccgctg gcagcggatg cagaaattac tatggaagcg aacccaggca cggtagaagc
     9361 ggatcgcttt gtcgattatc agcgtgctgg tgtgaaccgc atctctattg gtgtgcagag
     9421 ttttagcgaa gaaaagctga aacgactcgg gcgtattcat ggcccgcaag aagcgaaacg
     9481 agcggcaaag ctggcgagcg gtttagggtt acgtagtttt aaccttgatt tgatgcatgg
     9541 gctgccggat caatcactgg aagaggcgct aggcgatctg cgccaggcta ttgaactgaa
     9601 tccgccacat ctttcctggt atcaactgac catcgaacct aatacgctgt ttggctcgcg
     9661 ccctcctgta ctgccggacg atgacgcgct gtgggatatt ttcgaacagg ggcatcagtt
     9721 attaaccgca gcgggctatc agcagtatga aacatccgct tacgccaaac ccggttatca
     9781 gtgccagcac aatctcaact actggcgatt tggcgactac atcggtattg gctgcggcgc
     9841 gcacggcaaa gtgaccttcc cggatgggcg cattctgcgt accaccaaaa cgcgtcatcc
     9901 gcgtggtttt atgcagggaa gatatctgga aagccagcgt gatgtcgaag ctgcagataa
     9961 accgtttgag ttctttatga atcgcttccg tctgctggaa gccgcgccgc gcgtggagtt
    10021 tatccaatat actggccttt ctgaagaagt tattcgctct cagttagacg aggccatcgc
    10081 tcaaggttat ctcacagaat gtgcggatta ctggcagata accgagcatg gtaaattatt
    10141 cttaaactca cttcttgaat tattcctcgc tgagtaatca ttaatgcgcc tgcgggcgca
    10201 ttaat
//
LOCUS       DACADF010000153        10180 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.155, whole genome shotgun sequence.
ACCESSION   DACADF010000153 DACADF010000000
VERSION     DACADF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.155"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..1626)
                     /locus_tag="ISB30_003934"
     CDS             complement(<1..1626)
                     /locus_tag="ISB30_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP1991518.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVT
                     VDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQ"
     gene            complement(1572..1793)
                     /locus_tag="ISB30_003935"
     CDS             complement(1572..1793)
                     /locus_tag="ISB30_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991519.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(1838..3775)
                     /locus_tag="ISB30_003936"
     CDS             complement(1838..3775)
                     /locus_tag="ISB30_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP1991520.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(3839..5500)
                     /locus_tag="ISB30_003937"
     CDS             complement(3839..5500)
                     /locus_tag="ISB30_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP1991521.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGTNLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(5497..6060)
                     /locus_tag="ISB30_003938"
     CDS             complement(5497..6060)
                     /locus_tag="ISB30_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAP1991522.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNLEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRILVENLCIDPKLALDAAWRLAQFTHHKKGDTGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            complement(6351..6716)
                     /locus_tag="ISB30_003939"
     CDS             complement(6351..6716)
                     /locus_tag="ISB30_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP1991523.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCICGRVVDLCDSELDHRIALQFGGGNEETNLWTLCAECHRQKSAREAASGMPD
                     PTLPEVSGGSGREDDIIGL"
     gene            6758..6958
                     /locus_tag="ISB30_003940"
     CDS             6758..6958
                     /locus_tag="ISB30_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HAP1991524.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHIIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            complement(7157..7372)
                     /locus_tag="ISB30_003941"
     CDS             complement(7157..7372)
                     /locus_tag="ISB30_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991525.1"
                     /translation="MKKLRVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVLQPVC"
     gene            complement(7458..7643)
                     /locus_tag="ISB30_003942"
     CDS             complement(7458..7643)
                     /locus_tag="ISB30_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991526.1"
                     /translation="MRELKMKLCALMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            complement(7865..7951)
                     /locus_tag="ISB30_003943"
     CDS             complement(7865..7951)
                     /locus_tag="ISB30_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991527.1"
                     /translation="MSAELIRAMAFAIRIVAIAVLVWAIRWW"
     gene            complement(8506..9039)
                     /locus_tag="ISB30_003944"
     CDS             complement(8506..9039)
                     /locus_tag="ISB30_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAP1991528.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VFRRDQESALACWGIDR"
     gene            9145..9417
                     /locus_tag="ISB30_003945"
     CDS             9145..9417
                     /locus_tag="ISB30_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991529.1"
                     /translation="MDNNTISLQELLDSISRLREDVNTLTVAFSYLAFSIPREQMQST
                     LASIQFESCNPKWSQEQQDSFRRLAVLLDEKYAGKITISADSSENP"
     gene            complement(9383..9727)
                     /locus_tag="ISB30_003946"
     CDS             complement(9383..9727)
                     /locus_tag="ISB30_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HAP1991530.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKSPPKW"
     gene            complement(9732..9947)
                     /locus_tag="ISB30_003947"
     CDS             complement(9732..9947)
                     /locus_tag="ISB30_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP1991531.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT         2279 a         3135 c         2429 g         2337 t
ORIGIN      
        1 ctgcagtaca tccggatcat aatcacgacc atcacgcggt accggaatgg cccccacagc
       61 gtcatccacc atcgcctgca gaaccggacg cacctcatcc accgtcacat gcttctgtaa
      121 taccgccgac agggaagtca gtttctcttc aaacgcttgt gcctgcgagg ccatcttccc
      181 ctcaaatgtg cgctgtaaat ccgccagcac tgtggagaat tcttctccca gtgcacgaat
      241 aatggacagt tcccgttccg tcattttctc agtatccccc ctgaacatcg ctttcaccgc
      301 atcatgctct gtttcactga ttgccttatt accgtcagat gcgccgtcag gcagctgtga
      361 tgaaactgtt ttcccggcag acgcgaacgg atcctcacgg gcatcacgac gggacagcgc
      421 ctccagactg tagttctgct gctgaagata cagtgcatca ccgccggcca ggggcggcag
      481 gttctcccgt ttacgggcct cattgggcgt gagaagcgta tttttcaccg catcccccag
      541 cgttttcatg cgccgctcac tgtccattct cagcagcgtg gtgacatcaa attctgtact
      601 ctcgttttcc cccgtttcca gcgcctcatc cagtaacagt tcaatggact caatcagcgt
      661 ctgcaggcac tgggaataat actgctgctc cagcgcctcc acgttgtcac tggaaggcgg
      721 ttgtcccacg ccaatcttgt aggccgggac acggaacacc gaacagacaa tttcagcggt
      781 catcttcagt tgttccaccg tctgcgcatc cacaggtgaa aacgtcgtgg ggttgtattt
      841 tgccccgttg ctcagaatgg ccgttttccc cgcattttcg cctgtatacc cgctgtccca
      901 gttgctcttc agttttttcg cattttcttc cgtaatactg ccggggatct caatcacccc
      961 ggacggcctg ccgccatttc tgaaaaaaga cgtcgaattt tcctgaatat gatgcccctg
     1021 cgtggccgcc agcccggcgg catacaccgg cggcaatcct ataagcggat gaaaaaaaca
     1081 gttaaaccgg tcgtggatca cttcccgggc aggcaccgtc accgcctccg tgatcccgca
     1141 gttccggtcc ggcgtgatgc ggtagaacac ctcgccgtca tccgccacca gaggttcaac
     1201 ccggctccag tccagaatac gcagttcttt gatctgcccc cgggcattac ggattttcag
     1261 caccaccgta ttgccatgac gcagtttggc gttcagccac agttcaaaaa actggatgcg
     1321 gttctgctgg gcgttgggac gacgacagag gcgggcaata tccccccggc gcgtttccct
     1381 gcgtatccca tgcgcatccg tctgcataag acgcagccgc attttggcga tatcctggga
     1441 tatcagcgaa atacatgcaa acaccgcatg aaaggagagg acggcttcag gatcggcttt
     1501 cacgccctgc tgccaggcgc cggaaaaggg ctcagccacc gcctgaaaca ggctggtcca
     1561 gcccgcctct cttacgtcac gtcctgattt ctggtttttt cgggttcgcc gtaaaaggtt
     1621 ccacattcgc catgctccgc atcacgtttc tttttctgac ctgccggacg tcgcaccgtg
     1681 atgtactccg cctttcccag gcgaaccagc acctccgcac acggctgtgc cacatcacgg
     1741 atatccccgg cccgggcatc atgcgtgccc tgcagatatc ggatctttgc cataacctgt
     1801 tacgggaggc gcacgcctcc cgtcctcctt atcagactca gccgccggac gcactgccgt
     1861 agttcactcc ggtgatcacc gccaccgccg cagtacggcg acgacgccag ttgatccagc
     1921 gctccgcacg gatggccacg ctgcctgtct ggaacatgga aaccagctcc accggggacg
     1981 gcgtggtact gtcgccggtc ggctcagact gcatttccag tgatgcctcg cgggacatat
     2041 ccactgccac gccgccgtca tccgccagat aaatatccgg ggcattcacc agcaccagct
     2101 ggtcacccac gtactgggag acaatcaccg gcagcccctg gaaggagcca cccagcaggg
     2161 tcatgtccgg gtattccttc tgacccagcg catttttacg catggacagt gccagggcat
     2221 tggtgctgga catcagccag accgcaccgg tgggctgcag gtttgctgcc acaaactgtc
     2281 caaacgcagc ctctgcatcc gcatccgggt taccggttga tgccgtgccc ttcacatcat
     2341 gggtgatgga cgccggggag acatctgcca ctgcggcttt tttcgggtcc acaaagtctg
     2401 tatccagacg cgccaccacc gcttccgcca gcgcattacg gaccagtgca tcagcagccg
     2461 gactggaaaa acggatcaat tcttccgtca gtaccgcaat ggccgacacc ttcgcatgac
     2521 tgaaggtgat ggattcaaaa tcaaacttcg tcaggggttt tgccttaccc tcacccaccc
     2581 agccggcagc accgccggac acctgggcgt gcacacggat attgaatggc acctgacgaa
     2641 gtgcagggat cccgccctga ccaaatcgcc cgataatggt ctgcggacgc aggtaatcaa
     2701 taaagtcctg tgcgtattcc tgatattcag acaggctgcc tgcccactgc ggatccgtgg
     2761 tggtccccgc gcccactgcc gatttcagga catgatgcag acgactgtca tccggatact
     2821 gacgacgggc cacttccagg gcttcagatc ggacgccttt agccgcagcc agcgatttgg
     2881 caaagcgggc gaagccaatc cccttatcca gtttctgctc cacacggatc accggcgcag
     2941 aagccaccgc ggccacattc ccgttaccgg cctgtttcac cggctgcgcc gtggcggcct
     3001 taccggcttc cagttcacgc aggcgcttca ggtgcgcatc cacctgacgg atttccgctg
     3061 cggtgttgtc gtaatgctct tcctcctcca catccagcgt gcgcccttcc tctgcggctt
     3121 tggtcatgac ctcctcaagg gaggctgcca gcgctgcacg cttgttttca aaacttttaa
     3181 tctgttcgcc aatattcatt atggtctttt ccttatgaaa aacggttgtt gactgtgccg
     3241 cagcgccggc agaagatgcg attttcacca ccggtttccg gttgccggac gcggcagaaa
     3301 acgggcggtc gtaagattta atggtccgga tggtgcattc cgcattcgcg ggcacggtga
     3361 cggcagacac ctccatcagt tcccagcgca gaaaatgcag tccgcctccg tccagaaagg
     3421 tgtattcatg gggacggaag cccacggaca gccccctgac cagcccggtc ttaatggccg
     3481 cccagacctc atccagccgg gcagccagtt gcgacggcat atccggtacg ggcttcacca
     3541 gtgttgccgt gatttccagc ccttcgctga cccggcgcac cgtacactgc cccaccgggc
     3601 gggaatggtc atgctgccag agaaacggga tcgcactgcc aaactccgcg ccctccggct
     3661 ccaggatgtc accatcccga tccggagaag gcgttgacgc aatcccggtg atcacccgtt
     3721 catcctcact gaaggatttc accgtcagca gggaacaggc ccgtttaaga gtcacatcag
     3781 cctcctgaaa ataaaaaaac cgccggagcg gttcgtgatg gttacagtgt gaacagggtt
     3841 atatgaaaaa aaccgcatat tctttctttt tcggttccgg gttaagggac atcagggaga
     3901 ccgcattgaa cagcgccatc agcgggtcaa tttttccccg tccactggcc tgtttggtga
     3961 taagaatggc gttaccttta ggctccaccc gggcattgcc gacacaccag gccatcaggg
     4021 gctggtcacc atgcaccagc accccttcag ccagtttgcg ctcggtggtt ttaatggccc
     4081 cgcccagttt ccagccctgg cttatcccca ccacaattcc gtcggggatc ccggcttccg
     4141 ccagtgaatc cagaatctgc cccacccctg acgggtcaat accgatatgg tccagtaact
     4201 cagcctcatg aatgcgacgc acatattccg ccacttccgc cgtgtcatcc ccgacacgcc
     4261 ggacaatggt catatctcca caggcaacaa gatcctgaaa ccgggacgcc tcgctcttcc
     4321 gtcggaccac cgcggtttca tgcgcccagg catggcccca gcccagccat tcgcgggtct
     4381 cccggtcacg cccaatcaca tacatcccca gcagatcatc cagccctccg ccgtcaatcc
     4441 ccaccgtcac cacatcagca cgacgcagga tatcgtccag gctgatacaa cggccctgct
     4501 cttcccagaa atcagccccc gcccagcggt cagagcgcag ggcaagacca atttccacat
     4561 tggcgtgttt tgacatgaac ccccggaatg tctcttcacc ggcttcccgg gctttacggt
     4621 actcccggta cagaaaggcc tcatccactg aatagccgag attcggattg accatggcga
     4681 ggttttccat cagcaggtta gtcccgcttt ccaccatttc aggagggtgt tcaaatatca
     4741 ccggcagaaa gtgcggatca tgaattttgc cgtcgcgcac atcccgggcg tactgcagtt
     4801 tctgtctgaa caccccggcg ggcggttcat tcgactgggt ggtcgtatac accacaaacc
     4861 cttccgggcg ggaggcaagg ccgcctatgg cttcacgtaa catgtcctcc gccttgcact
     4921 gcttgccaaa cagccacaac tcatcaatca gcgtacccac ggacttgata ccggacaccg
     4981 tattcggatc ggctgccacc accttcaggg tggtgtccgt cactcggtgg gtgatggtcc
     5041 ggatatgggt ctgtacctgg cagaggtcat ccagatcatc gtcacgtcgt accatatccc
     5101 gggcagggtt gaaggcgttg gccgccacct ccacagtcgg ggccagaatc gtgtaacccg
     5161 ccgcctgccg ccagttcagt aacagtgcag tcatcatgat ccccgcggcc agcgtggact
     5221 tcgagttttt cttggggata aggataaaaa cttccttgat atggcgtaca ccggtctgcg
     5281 catcgtagga gccaaacagg gccgccacca ggtcaaacac ccacggtgca caggactccc
     5341 cgaacgtagg gctaccaggt gcatccacaa tccgcagttg tttaaaaatc gccagggcat
     5401 gtgcggcctc gtccggataa atcggatccg gaataatcga cagccccttt ttcaggcgct
     5461 ctgcccagtc cgggcaggct gtgctccata caggtatcat ccgttgccct cattatcgtt
     5521 attcaccacc agtcgggggg gcggtggcac cgcaaaacgg ttagccgctt ttttcgcggc
     5581 atcacctttt gccgattttt tcccggtatc ccctttttta tggtgcgtga actgcgccag
     5641 acgccaggcc gcatccagtg ccagtttcgg atcaatgcag aggttttcca ccaggatccg
     5701 ccccatggct ttcaccggat cgggaagacc atcctccata tattcaatac caggagacat
     5761 caccgcggac ggtggcatct ccagattgtt ttcgtccggc tgtggtattg cagccgcctc
     5821 acggcgacgg ggtttatcct cctgctctga ttttttctgc cggtaaacag gaacctcatc
     5881 cacctccacc gtctcgcatt gtttacgggc tataaacgca agcacctcag gatcttttgc
     5941 cagctgcgag cctttaaccc tggcggtctt cgccgaataa ccggcggcaa tggctgacgc
     6001 tgttttgttt ttcccggaca tgagcgccag cgcaaatttt cgtttttgcg ttgtcagcac
     6061 agcctcctcc cgggtccaga acgcactcag ccgggtatgg ttcagcccat ttttcccggc
     6121 gtctcatgcc gcaaatgtta actgctgcct ggttaacatt tgctgaaaaa gcctgttaac
     6181 attttttccg cacaacaaac tgaataataa agataaaaac cgcaaaaatg cccgggcagc
     6241 cagttaacat gttaactgcc ctgaaacggg aattttttct ctgcgtgaga gggggcgcgg
     6301 tgtccaaagc gatcgttttt tacgccggat gatacccccc ccgggttggg ttacagtccg
     6361 atgatgtcgt cctctctgcc actacctccg gacacctccg gcagcgtcgg gtccggcata
     6421 ccactcgccg cttcacgagc agacttttgt cgatggcatt cggcacagag cgtccaaaga
     6481 ttcgtctcct cattaccacc accgaactga agtgcaattc ggtgatcgag ttcactgtca
     6541 cagaggtcaa ccacacgacc acagatacag cactgcccgg cgtccctcag ccagatatga
     6601 cgcttgaggg aaacacgtgc actgccactg acacgacgct gctccccctt cagaatattc
     6661 acccgtcggg tattcagtgt tttgattctg ctctggagtg tacgaagctc agccatgtaa
     6721 aatccccgtc atatggcaat cagtaaagga aataaatatg tcatcgaaaa accggacccg
     6781 cagaaccaca acccgcaata tccgtttccc caatcacata attgaacaga tcaacatcgc
     6841 ccttgagcat aaagggtccg gtaacttttc agcgtgggtt attgaagcct gcaggagaag
     6901 gctggcaaca gatgcaacgc atctgcgccc ggccagcatg acaaataacg agaaatgaac
     6961 gttcggtttc ttccaccatc gcaccggaca ggcgactatg aggggacaac gccgcgctcc
     7021 gttaacgcgg taaaccccgg tgtgtatcgt ttttgattat ccccgcacac tcgcgcagag
     7081 gagtctccct gtcgggctgc ggtctctgtt aatgcaggaa tacggcgaca ataccgcgca
     7141 tggataataa ggtcgctcaa cacactggct gtaataccat gcggcattta gcggcattca
     7201 tcgtacactc cacggttagc tcttcattcg tggcattcac ctgaaaggtc cgggagtgta
     7261 attgcgtaca tttaccactg aacgaacctt caacaagaac acgaccacgc tgcaaaatac
     7321 ggaacggaat tgttccctga aaaggttcta cggttacccg taatttcttc atgtatcctc
     7381 cggataataa aaagccagct tagtgcactg agtgcggata tattcctgcg ccccttccag
     7441 ctgcttctgc attgtcatca accgttctct gaggatgaaa taatcccgtt cagcggtgtc
     7501 tgccagtcgg gggccggttg cattatccac gctggaggtg ccggtggctt cacgcacggt
     7561 accggggcag gtggcgttga cccgcaggcg cttacgacca gcggcaacat cagcgcgcag
     7621 agtttcattt tcagctctcg catcggctaa ttccttcgtg tatctggcat caagcgcagc
     7681 gacatctcgc tggcgctgct gcatgtcagt aatagtggca ttcgccagtt tcagctcact
     7741 gactttttta tcgcgctgcg ctttgtaggt gatggcgtta tcgcggtaat gattcagccc
     7801 cagactaagc acaccacagg ctaccagcat gacaataatc accacacaca gaacacggtt
     7861 catatcacca ccaacggatt gcccagacca gaacagcaat ggccacaata cgaatggcaa
     7921 atgccattgc ccgaataagt tcagcactca tctttttaaa gttcacgatt tcagcgcaat
     7981 gaccagtttt gccagcccat acagcatcgg agacacagca ataccaacag ccacccactt
     8041 aatagcaaaa gccagcgctc tgctgatgtc atcagtcact gtcaccccag cagccccgac
     8101 gaagacaaca tcacccaggc gagggacaga aaaagagcaa ccagcattag tgaaaatgaa
     8161 ataccgacaa tcacacacag gacctttgcc ggcgttatga gtttgtctga catagctacc
     8221 ccttaattgc cacaattaac tgggatacta cccataaaaa agggatgctc cagaccagca
     8281 aaaatttcca gtttggtaat tgactaatca tgagtcgcaa ctccctaatc agtttgctaa
     8341 aatcaatcaa ggcagcctcc catagcttac tgccataaaa acaaaacccc gcttgctgcc
     8401 aacaaacggg gtttttactt ttattcactt aggttttgcc agttcacagg acttcgtgtt
     8461 atccgcccgc gttggccaac ctcatttttc agcaaaatat tctgcttatc tgtcgattcc
     8521 ccagcacgcc agcgcgctct cctggtcacg acggaatacc tgaccgtaac agttatttga
     8581 gcgaatacgg cagtctctgc caccgtcctt aatccaccag cgaatcgctt cgcatgctcc
     8641 cctgcgatca cctgcattaa ttcgtctgta aaacgtcgac gggaaacact taccgggacc
     8701 aatgttgtac ggacagaatg acgcgatccc cgctttctgg ggttcggtca atggcacttt
     8761 gatgtttttc tccacccatg ccagcgcctt atcacgctca atggcgttaa cccggtcgca
     8821 tttttccttc gacaacttca tgcccggaac gacaggtttg ccatccacca ggatggcacc
     8881 gcggcagatg gtccagatac ccgcgccatc acggtatgcc gtggtgtggt taccttcctt
     8941 ttcatccaga aactggtcga gaatgtcagg cgcaggcgca ccagcggcaa tcagcgccag
     9001 aacggcagcc gacaggccgt atctgatttt tgcgttcatg gatatttatc aggatttatc
     9061 ggtttctgag ccctggatat gtttatcagt tccagcctgt tgcctcaggc tgctaacagg
     9121 tcaatacaat catgaggatt atttatggac aataacacca tttctctaca ggagttgctc
     9181 gacagcattt ccaggcttcg ggaagacgtg aataccctta ccgtcgcctt ctcatatctg
     9241 gcattctcaa ttccaaggga acagatgcaa tcaacgctgg catcaatcca gtttgaatca
     9301 tgcaatccca aatggtctca ggaacaacaa gactctttca ggcggcttgc tgtattactg
     9361 gatgaaaaat atgctggtaa aattaccatt tcggcggact cttcagagaa cccgtaatta
     9421 ttcccggtag ttttcctctg taggttatca acacatcctg cgcctctaaa attacggggc
     9481 gcttttccgg cgactgctca tccccttcac ataacccggc agcaacatcc aggaagacct
     9541 gtctgatgct ccttctggct gctgcctcat aaaactccag cgcggcacct tcaacacggt
     9601 ccagcgagat gtccaggtca aaaatttcac cgtcaaagcg ttttttgtcc cgtaacgcta
     9661 aagttaccgt aactttattc tcaaaattgc ggatcccttt cacaatcagt tcatagtttt
     9721 gagtcattga attactctcc ccgtgccgcc ttacgacggt cctctctgat tttgaaatac
     9781 aggttagtca gatatgtcag cagcccaaac agcagactcc ccagcacgcc tattgccgcc
     9841 cactgagacg gggaaaccct gtccagcaac tgcaggaacc agtagcccgt tcccaccgct
     9901 gacgtggtgt atgacacacc tgttgtgatt ttttccatct ggtacatacc ccgtctcccg
     9961 ttatccggaa gctgacaaca ataaaaaaag ccaccagtta agtactgatg gctctgataa
    10021 ctcatgcagg catctcagac gacccactga cactaccggt gagtttaacg ataccttcca
    10081 tttgactggc tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct
    10141 atctcaacgc catacgttgc atttttggta atatccgtca
//
LOCUS       DACADF010000154        10072 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.156, whole genome shotgun sequence.
ACCESSION   DACADF010000154 DACADF010000000
VERSION     DACADF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.156"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(391..2964)
                     /gene="clpB"
                     /locus_tag="ISB30_003948"
     CDS             complement(391..2964)
                     /gene="clpB"
                     /locus_tag="ISB30_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP1991532.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(3094..3825)
                     /gene="pgeF"
                     /locus_tag="ISB30_003949"
     CDS             complement(3094..3825)
                     /gene="pgeF"
                     /locus_tag="ISB30_003949"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAP1991533.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVGENRKRLFAAGNLPSKPVWLEQVHGKDVLKLIGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3822..4802)
                     /gene="rluD"
                     /locus_tag="ISB30_003950"
     CDS             complement(3822..4802)
                     /gene="rluD"
                     /locus_tag="ISB30_003950"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAP1991534.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEEMRADFEEHKDEVDWL"
     gene            4937..5674
                     /gene="bamD"
                     /locus_tag="ISB30_003951"
     CDS             4937..5674
                     /gene="bamD"
                     /locus_tag="ISB30_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAP1991535.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5944..6285
                     /gene="raiA"
                     /locus_tag="ISB30_003952"
     CDS             5944..6285
                     /gene="raiA"
                     /locus_tag="ISB30_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP1991536.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            6389..6436
                     /locus_tag="ISB30_003953"
     CDS             6389..6436
                     /locus_tag="ISB30_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991537.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6535..7695
                     /gene="pheA"
                     /locus_tag="ISB30_003954"
     CDS             6535..7695
                     /gene="pheA"
                     /locus_tag="ISB30_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAP1991538.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELVVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7738..8859)
                     /gene="tyrA"
                     /locus_tag="ISB30_003955"
     CDS             complement(7738..8859)
                     /gene="tyrA"
                     /locus_tag="ISB30_003955"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAP1991539.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLILSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8870..9940)
                     /gene="aroF"
                     /locus_tag="ISB30_003956"
     CDS             complement(8870..9940)
                     /gene="aroF"
                     /locus_tag="ISB30_003956"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAP1991540.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
BASE COUNT         2556 a         2679 c         2349 g         2488 t
ORIGIN      
        1 tgagacttgg tattcatttt tcgtcttgcg acgttaagaa tccgtatctt cgagtgccca
       61 cacagattgt ctgataaatt gttaaagagc agtgccgctt cgctttttct cagcggcgcg
      121 gggtgtgcat aatacgcttt cccgctacag agtcaagcat ttatttttgc tttctctgcc
      181 ggaattctca ggagaacccc gctcacccgg cggcgtgttt gccgttgttc cgtgtcagtg
      241 gtggcgcatt atagggagtt attccggcct gacaagcgaa aaatataaaa actttatcgt
      301 tcgctcattt ttcaggcaaa acatcttaaa tatagtcttt tccgtctaac ttatagacaa
      361 aaacgagccc cgaagggctc gttttatcat ttactggacg gcgacaatcc ggtcttcatt
      421 aacttccagg cgaatcactt tacccggaac caattcacca gacagtattt gctgtgccag
      481 cgggttttca atctgctgct gaattgcacg tttcagagga cgtgcgccat agaccggatc
      541 gtaaccgttc tcgctcagca gtttcagcgc ctcgtcagaa atgtggattt cataaccacg
      601 ttcttccaga cgtttataca gacgtttcaa ctgaatctgc gcaatcgacg caatgtgctg
      661 ttcacccagc ggatggaaga ccaccacttc atcgatacgg ttaatgaatt ccggacggaa
      721 gttatggcta accacaccga gcaccagctc tttcatgtgc gcataatcca gttcaccgaa
      781 gcgttcctga atcaaatcgg aaccgaggtt agaggtcata atgacaaccg tattacggaa
      841 gtcgaccgtt ctcccctgcc cgtcagtcag acgcccatca tccagtacct gcaacagaat
      901 gttgaagaca tccggatgcg ctttttccac ttcatccagc agaatgacgg aatacggacg
      961 acgacgcacc gcttcggtca ggtagccacc ttcttcataa ccgacatatc ccggaggcgc
     1021 accaaccaga cgagacaccg agtgtttctc cataaactcg gacatatcga tacggaccat
     1081 cgcctcgtcg ctatcaaaca taaagttcgc tagcgcctta caaagctcag ttttccccac
     1141 accggttggg ccgaggaaca ggaatgaacc aatcgggcga tttggatccg ccagccccgc
     1201 acggctacga cgaatagcgt tagataccgc atcaaccgct tcgttctgac caataacgcg
     1261 atggtgcagt tcttgctcca tacgcagcag tttttcgcgc tcgctttcca tcatgcgaga
     1321 aaccggaatc cccgtccaac gcgccagcac ttcagcaatt tcggcatcgg tcactttatt
     1381 acgcaacaga cgcatagttt tgccttcgag ctgcgttgcg gcttccagtt gcttttccag
     1441 ttccgggatt ttgccgtatt gcagttcaga catccgcgcc aggtcaccca cacggcgagc
     1501 ctgttcaata gcaattttcg cctgttccag ttccgcttta atggtctgcg taccagaaag
     1561 cgaggctttc tctgctttcc actcttcttc taactcggag tactgacgtt ctttgtcgct
     1621 cagttcttcg ttgagcatat ccaggcgttt tttactggct tcatcagact ctttcattaa
     1681 cgcctgttgt tccagtttga gctggatgat acggcgatcg agtcggtcga gttcttctgg
     1741 ttttgagtca atctgcatac gaatgctgga tgctgcttca tcgatcaggt cgatggcttt
     1801 atccggcagc tgacggtcag caatgtagcg atgagacaac gtcgctgctg caacaattgc
     1861 cgggtcagta atttgcacat ggtggtgcag ctcgtaacgt tctttcaggc ctcgcagaat
     1921 agcaatggta tcttcaacag aaggctcggc aacaaacact ttctggaaac gacgttccag
     1981 cgcagcatct ttttcgatgt actggcgata ttcgtcaagc gtcgtggcac ctacgcagtg
     2041 caattcacca cgcgccaacg ctggtttcag catgtttccg gcgtccattg cgccatcggc
     2101 tttacccgcg ccgaccatgg tatgtaattc gtcgataaac aggatgacgt tgccttcctg
     2161 tttagcaaga tcgttaagta cgccttttaa acgttcttca aactcaccgc gatatttcgc
     2221 cccagccacc agcgcgccca tatccagcgc cagtacgcgg cggcctttca acccttcggg
     2281 cacttcgccg ttgataatac gctgcgccag accttcaacg atggcagttt taccgacgcc
     2341 gggttcacca atcaataccg ggttattttt agtacgacgt tgcagcacct gaatggtacg
     2401 gcgaatttct tcatcacgac caatcaccgg atcgagtttg ccttgttcgg ctcgttcggt
     2461 aaggtcgatg gtatattttt tcaaagcctg acgttggtct tcagcacctt gatcgttcac
     2521 gctttcacct ccacgcattt gttcaatcgc ttgagtaatg ttggcggtgg tcgcccctgc
     2581 tgctttcagg atgtcggcca gcgtgccgcg agactcaagt gccgccagaa cgaacagttc
     2641 tgacgagata aagttatcac cacgtttttg cgccagcttg tcgcaaagat taagaacgcg
     2701 caccagatcc tgtgatggct ggacatcacc accagtacct tcaacctgtg gtaaacgatt
     2761 taatgcctga ttgatatctg tgcgcaactg gccagcatta atgccagcgg atgttaataa
     2821 aggactaacc gaaccccctt cctgattcag cagggcgctc attaaatgaa gtggttcgat
     2881 gaattggttg tcgtgcccga gtgcaagtga ttgggcatcg gcaagagcaa gctggaattt
     2941 attagtaaga cgatccagac gcataactcc tcccataacg gatcaaagtt gctactggag
     3001 attaaatgag gtcatccctc aattattcaa ggttattgac cagattaatg tgaaaagaaa
     3061 atcacgcgta ccggatcgtc ttgattcttt aggttatatc agccaaatga aacttgccat
     3121 acgaccggtg gtcttgtcgc gacgataaga gaagaaagtc tcattttccg tatatgtaca
     3181 acggtcgccg ccgaaaattt gttcaacacc aacgctcgcc agacgctgcc gggcaagctg
     3241 ataaatatcc gccagatact tatcaccatg ctgaatgaaa gctgtactcg ctttggcgtc
     3301 tactgccata aacgcctcgc gaacctccgc ccccacttcg aacgcacgtg gcccaattgc
     3361 cggccctaac caggcgagaa tattttccgg attatcagca aaacaggaaa ccgtctcttc
     3421 caacacgcct gcgcacagtc cacgccagcc agcatgagcg gcggcgacct ccgttcccgc
     3481 acgattgcaa aacagcacgg gcaggcagtc ggcagtcatc actgcgcaaa ccgtgccagg
     3541 cgtattgcta taagaggcat ctgctcgttt tgaggcataa ggttcgccaa taagcttaag
     3601 cacgtctttg ccgtgtacct gctcaagcca gaccggttta gaaggcaaat tgcccgcagc
     3661 aaaaagtcgc ttgcgattct ccccaacgtg atccgggtta tcgccacaat gggcaccgag
     3721 gttgagcgag tcatacgggg gtaagctcac accgccgata cgggtagagc tacaagccgc
     3781 aacacctttt ggcagcggcc actgcgggac aatcagctta ctcataacca gtccacttca
     3841 tccttatgct cttcgaaatc ggcgcgcatc tcctcaatca gctccaccat atcttgtgga
     3901 ataggcgcat gccattccat ttcgatgccg gagatcgggt gataaagacg cagcatggtt
     3961 gcatgaagcg cctgacggtc aaacttacgc agcgtggaga taaacgcttc cgaagcacct
     4021 tttggcggac gcggacgtcc accataaacc ggatcgccca ccagcggatg agtgatatgg
     4081 gccatatgca cgcggatctg gtgcgtacgt ccggtttcca gacgcaaccg cagacgcgtg
     4141 tgcacacgga agtgttccat gatgcgatag tgagtgaccg ccggtttacc catgggatgt
     4201 accgccatgt gagtacgttt ggtcgggtgg cggctgattg gttcatctac tgtaccgcca
     4261 gcggtcatat gaccaattgc caccgcttca tactcacgtg taatttcacg ccgttgcaaa
     4321 gattcgacta aacgcgtctg agccggaacg gtttttgcca caaccatcag gccagtggtg
     4381 tctttatcca gacgatggac aatgcccgca cgcggtacat cggcaatggg cggataataa
     4441 tgaagcaatg cattcagtac cgtgccatcc gggttacccg cgccaggatg taccaccagg
     4501 tcgcgcggtt tattaataac gatgatatct tcatcttcat agacgatatc cagcgggata
     4561 tcctgcggtt caaaacgcgc ttcttcttca atctcagcgt tgatggcaac ctgctcgcca
     4621 cccaatactt tttctttcgg cttatcacac actttgccgt taaccagcac acgctggtcg
     4681 aggatccatt cttttatccg cgaacgtgaa taatccggga acatttcggc caaagcctga
     4741 tctaagcgtt gaccgagttg gttttcggac accgttgcag tgagctgtac tcgttgtgcc
     4801 atatatactg cttcttcgtt tatcgttggg gttttacggc tttgccgttt aatatagtgt
     4861 gctattgtag ctggtcttaa ccgggagcag gaacagagaa tctcccgtat tacattttga
     4921 ggaaagtcaa aacgtcatga cgcgcatgaa atatctggtg gcagccgcca cactaagcct
     4981 gtttttggcg ggttgctcgg ggtcaaagga agaagtacct gataatccgc caaatgaaat
     5041 ttacgcgact gcacaacaaa agctgcagga cggtaactgg agacaggcaa taacgcaact
     5101 ggaagcgtta gataatcgct atccgtttgg tccgtattcg cagcaggtgc agctggatct
     5161 catctacgcc tactataaaa acgccgattt gccgttagca caggctgcca tcgatcgttt
     5221 tattcgcctt aacccgaccc atccgaatat cgattatgtc atgtacatgc gtggcctgac
     5281 caatatggcg ctcgatgaca gtgcactgca agggttcttt ggcgtcgacc gtagcgatcg
     5341 cgatcctcaa catgcacgag ctgcgtttag tgacttttcc aaactggtgc gcggctatcc
     5401 gaacagtcag tacaccaccg atgccaccaa acgtctggta ttcctgaaag atcgtctggc
     5461 gaaatatgaa tactccgttg ccgagtatta tacagaacgt ggtgcatggg ttgctgtcgt
     5521 taaccgcgta gaaggcatgt tgcgcgacta tccggatacc caggctacgc gtgatgcgct
     5581 gccgctgatg gaaaatgcat accgtcagat gcagatgaat gcgcaagctg aaaaagtagc
     5641 gaaaatcatc gccgcaaaca gcagcaatac ataacagaaa cctgaaacac aaaacggcag
     5701 ccttgagctg ccgttttttt attctgttag ttgtgaaact gaagcgattt agtcactatc
     5761 gatctcatca aatatggccc gctttgagat attcctcaag taaaaaaaca cctcttcctg
     5821 cgatttctca caaaaaagat tcgttgacaa aaagtgacaa aattatgaga tttccatcac
     5881 acattttgac atcaggaacg ttatgctgaa ttcaccaaga cgggaagaca agaggtaaaa
     5941 tttatgacaa tgaacattac cagcaaacaa atggaaatta ctccggccat ccgccaacat
     6001 gtcgcagacc gtctcgccaa actggaaaaa tggcaaacac atctgattaa tccacatatc
     6061 attctgtcca aagagccaca agggtttgtt gctgacgcca caatcaatac acctaacggc
     6121 gttctggttg ccagtggtaa acatgaagat atgtacaccg caattaacga attgatcaac
     6181 aagctggaac ggcagctcaa taaactgcag cacaaaggcg aagcacgtcg tgccgcaaca
     6241 tcggtgaaag acgccaactt cgtcgaagaa gttgaagaag agtagtcctt tatattgagt
     6301 gtatcgccaa cgcgccttcg ggcgcgtttt ttgttgacag cgtgaaaaca gtacgggtac
     6361 tgtactaaag tcacttaagg aaacaaacat gaaacacaca ccgtttttct tcgcattctt
     6421 ttttaccttc ccctgaatgg gaggcgtttc gtcgtgtgaa acagaatgcg aagacgaaca
     6481 ataaggcctc ccaaatcggg gggccttttt tattgataac aaaaaggcaa cactatgaca
     6541 tcggaaaacc cgttactggc gctgcgagaa aaaatcagcg cgctggatga aaaattatta
     6601 gcattactgg cagagcgacg cgaactggtc gtcgaggtgg gaaaagccaa actgctttcg
     6661 catcgcccgg tacgtgatat tgatcgtgaa cgcgatttgc tggaaagatt aattacgctc
     6721 ggtaaagcgc accatctgga cgcccattac attactcgcc tgttccagct catcattgaa
     6781 gattccgtat taactcagca ggctttactc cagcaacatc tcaataaaat taatccgcac
     6841 tcagcacgca tcgcttttct cggccccaaa ggttcttatt cccatcttgc ggcgcgccag
     6901 tatgctgccc gtcactttga gcaattcatt gaaagtggct gcgccaaatt tgccgatatt
     6961 tttaatcagg tggaaaccgg ccaggccgac tatgccgtcg taccgattga aaataccagc
     7021 tccggtgcca taaacgacgt ttacgatctg ctgcaacata ccagtttgtc gattgttggc
     7081 gagatgacgt taactatcga tcattgtttg ttggtctccg gcactactga tttatccacc
     7141 atcaatacgg tctacagcca tccgcagccg ttccagcaat gcagcaaatt ccttaatcgt
     7201 tatccgcact ggaagattga atataccgaa agtacgtctg cggcaatgga aaaggttgca
     7261 caggcaaaat caccgcatgt tgctgcgttg ggaagcgaag ctggcggcac tttgtacggt
     7321 ttgcaggtac tggagcgtat tgaagcgaat cagcgacaaa acttcacccg atttgtggtg
     7381 ttggcacgta aagccattaa cgtttctgac caggttccgg cgaaaacgac gttgttaatg
     7441 gcgaccggac aacaagctgg tgcactggtt gaagcgttgc tggtactgcg caaccacagt
     7501 ctaattatga cccgtctgga atcacgtccg attcacggta atccgtggga agagatgttt
     7561 tatctggata ttcaggccaa tcttgaatca gcggaaatgc aaaaagcatt gaaagagtta
     7621 ggggaaatca cccgttcgat gaaggtattg ggctgttacc caagtgagaa cgtagtgcct
     7681 gttgatccaa cctgatgaaa aagtgccgga tgatgtgaat catccggcac tggattatta
     7741 ctggcgattg tcattcgcct gacgcaataa cacgcggctt tcactctgaa aacgctgtgc
     7801 gtaatcgccg aaccagtgct ccaccttgcg gaaactgtca ataaacgcct gcttatcgcc
     7861 ctgctccagc aactcaatcg cctcgccgaa acgcttatag taacgtttga ttaacgccag
     7921 attacgctct gacgacataa taatgtcggc ataaagctgc ggatcctgag caaacagtcg
     7981 cccgaccatc gccagctcaa ggcggtaaat cggcgaagag agcgccagta gttgctcaag
     8041 ctgaacattt tcttctgcca ggtgcagccc ataagcaaaa gtagcaaagt ggcgcagagc
     8101 ctgaataaac gccatattct gatcgtgctc gacagcgcta atacgatgca accgagcgcc
     8161 ccagacctga atttgctcca gaaaccattg gtatgcttcc ggcttacgtc catcacacca
     8221 gaccacaact tgctttgcca ggctaccgct gtccgggccg aacatcgggt gtaaccccag
     8281 caccgggcca tcgtgcgccg ccagcatggc ctgtaatggt ccatttttca ctgatgccag
     8341 atcaaccaga atacaatctt tcggtaaagg cggtaatttg ccgataactt gctcagtaac
     8401 gtggatgggc acactaacaa tcaccattcc ggcatcggca acaatatcag ccgctcgatc
     8461 ccagtcatgt tgctccagaa tccgcacctg ataacccgag agtatcagca tcttctcgaa
     8521 cagacgtccc atctgaccgc caccaccgac gataaccacc gggcgcagtg acggacaaag
     8581 cgttttaaat cctttgtcgt tttcactgga gtaagattca cgcatcaccc gacgcaaaac
     8641 atcctcaatc agatctggcg gtacacccag agcttccgcc tccgcacgac gcgaggccaa
     8701 catagatgcc tcgcgctccg gaacataaat aggcagtcca aatcggcttt tcacctcgcc
     8761 cacttcagca accaattcca gacgcttcgc taataaattc agcagcgctt tatcgacttc
     8821 atcaatttga tcgcgtaatg cggtcaactc agcaaccata ataaacctct taagccacgc
     8881 gagccgtcag ctgcccgttc agatcctgat gaatttcacg cagcaaggca tcggtcattt
     8941 cccagctaat gcaggcatcg gttacggata caccgtattt catttcactg cgcggttgct
     9001 cggaagactg attgccctcg tggatattac tttcgatcat cagaccaata attgagcgat
     9061 tgccatcttt gatttgagca accacggatt ctgccaccgc aggctgacgg cgataatctt
     9121 tattggaatt accgtggctg caatctacca tcagagacgg gcgcagtccc gcctgttcca
     9181 tctctttttc acattgcgca acatccgcag ggctatagtt tggcgcttta ccaccgcgca
     9241 ggatcacatg cccgtccgga ttcccctgag tttgtagcaa cgcaacctgc cctgcctggt
     9301 taatgccaac aaaacggtgc ggctgggcgg cggcgcgcat agcgttaatt gccgttgcca
     9361 gactgccatc ggtgccgttt ttaaaaccaa ccggcatgga aagcccggag gccatttcac
     9421 ggtgagtttg cgattccgtt gtacgagcac caattgctga ccagctaaac agatcgccca
     9481 ggtattgcgg gctattcgga tctaacgctt ccgtcgccag tggcagtccc atattcacca
     9541 gctcaagcag caatttacgc gcgatctgca gcccggcttc tacatcaaaa gagccatcca
     9601 tatggggatc gttaattaac cctttccagc cgacagtggt acggggtttt tcaaaataga
     9661 cgcgcattac cagatagagg ctatcgctga cctctgcggc aagggcttta aatcgacgag
     9721 catattccag agcagtttcc ggatcatgaa tggaacaagg accacatact accagaagac
     9781 gaggatcgcg cccggcgata atatctgaaa tggttttacg cgagtcagca atctgggctt
     9841 cttgttgcag gcttaatgga aaagcggcct tcagttgttc cggagtcatt aaaacctgtt
     9901 cgtcggtaat atgtacgtta ttcagcgcgt ctttttgcat gatggcgatc ctgtttatgc
     9961 tcgtttgcga tagttgatcc tcagcgagga tgacgtaacg ataacacata aagtaaagtt
    10021 ttcaatccat atttcgtaca catttcttta cgtaagcaat ttattcgcgc tt
//
LOCUS       DACADF010000155         9957 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.157, whole genome shotgun sequence.
ACCESSION   DACADF010000155 DACADF010000000
VERSION     DACADF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.157"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..1173)
                     /locus_tag="ISB30_003957"
     CDS             complement(<1..1173)
                     /locus_tag="ISB30_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1991541.1"
                     /translation="MLLSDIQIKRAKPKDKPYTLNDGMGLSLLIDTAGSKGWRFRYRF
                     AGKPKMISFGVYGDVSLAQARTKRDEARSMLANGINPSEARKADKIALQFAHENSFES
                     VAREWHSSKKATWSEGYAKEVLNCMEKDIFPFIGQRPIEQIEPLELLTVLQKIEKRGA
                     LEQTSKIRRRCGEVLRYAVATGRAKYNFAPDLAIALNKPKTQHFPFLTESELPEFVNA
                     LDNYQGSLVTKYATQLLMLTGVRTIELRAAEWAEFDLDNALWEIPKERMKKRRPHLVP
                     LSTQAINILKKLQEITGNYSLVFPGRNDVSKPMSEASINKVIKLLGYHGRLTGHGFRH
                     TMSTILHEHGFESAWIEMQLAHVDKNSIRGTYNHAQYIKDRQVMMEWYASLLNTTCY"
     gene            complement(1334..1408)
                     /locus_tag="ISB30_003958"
     tRNA            complement(1334..1408)
                     /locus_tag="ISB30_003958"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1373..1375),aa:Arg,seq:cct)
     gene            complement(1484..2416)
                     /locus_tag="ISB30_003959"
     CDS             complement(1484..2416)
                     /locus_tag="ISB30_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAP1991542.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGIVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2710..3465
                     /gene="mlaA"
                     /locus_tag="ISB30_003960"
     CDS             2710..3465
                     /gene="mlaA"
                     /locus_tag="ISB30_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAP1991543.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(3527..4867)
                     /gene="fadL"
                     /locus_tag="ISB30_003961"
     CDS             complement(3527..4867)
                     /gene="fadL"
                     /locus_tag="ISB30_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAP1991544.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDITGHSNLTGQSANAKNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFTDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERYAGSLGPLLSQQLIAQGVPASLTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFDGDYKSSINPNLNNILGNMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSMAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYYDKNWTFRTGIAYDDSPVPADKRSISIPDQDRLWLSA
                     GLTYAFNEDASIDVGASYMHGQKVKFTEGEGAAAYSFESEGKAWLFGTNFNYAF"
     gene            5239..5523
                     /locus_tag="ISB30_003962"
     CDS             5239..5523
                     /locus_tag="ISB30_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAP1991545.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5704..7014
                     /gene="fadI"
                     /locus_tag="ISB30_003963"
     CDS             5704..7014
                     /gene="fadI"
                     /locus_tag="ISB30_003963"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAP1991546.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     GDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDESKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7014..9158
                     /gene="fadJ"
                     /locus_tag="ISB30_003964"
     CDS             7014..9158
                     /gene="fadJ"
                     /locus_tag="ISB30_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAP1991547.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRNIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKFSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKMPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSASANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGVQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCDRLVEMSERGESFWKTTATDLQ"
     gene            9361..9846
                     /gene="sixA"
                     /locus_tag="ISB30_003965"
     CDS             9361..9846
                     /gene="sixA"
                     /locus_tag="ISB30_003965"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAP1991548.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTHGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2439 a         2377 c         2539 g         2602 t
ORIGIN      
        1 atagcaggtc gtatttaata agctagcgta ccattccatc attacttgtc gatctttaat
       61 atattgcgca tgattgtaag tacctcgaat agagttctta tcaacatgcg caagctgcat
      121 ttcaatccag gcactttcaa acccatgctc atgcaagatg gtgctcatgg tatgcctgaa
      181 accgtgaccc gttaaccgac cgtgataacc cagtagtttt atcactttgt tgatgctggc
      241 ttcgctcatt ggtttactga cgtcattccg gccagggaaa acgaggctgt aattccctgt
      301 tatttcttgc agctttttca ggatattgat cgcctgagtt gataacggaa ccagatgggg
      361 gcggcgtttc ttcatccgtt ctttggggat ctcccataag gcattatcta aatcaaactc
      421 agcccattcc gctgcacgta gttcaattgt tcgcacgcct gttagcatca acagctgcgt
      481 ggcgtatttg gtaactaaac tgccctgata gttatccaga gcattaacga actcgggcaa
      541 ctcgctttca gtcaggaacg ggaagtgttg ggttttaggt ttgttgagtg cgatagctaa
      601 atcgggtgca aaattatact ttgctctacc agtggcaaca gcataacgaa gcacttcacc
      661 acagcggcgg cggatcttac tggtttgctc tagcgcacct cttttctcta ttttttgcag
      721 gacggttagc agttctaatg gttcaatctg ctcaattggg cgctggccga taaaaggaaa
      781 aatatccttt tccatacaat tgagaacctc ttttgcataa ccctctgacc aggtggcttt
      841 tttcgatgag tgccattctc tggccacaga ctcaaagctg ttctcatgcg cgaactgcaa
      901 agcaatctta tctgcttttc tggcttcact tgggtttatc ccgttggcta acatcgaacg
      961 cgcttcatca cgtttggtac gtgcctgtgc caacgacaca tcgccatata cgccaaagga
     1021 aatcatctta ggcttacctg caaagcgata gcgaaaacgc cagcctttac tgcccgctgt
     1081 gtcgatcagg agtgatagcc ccataccatc gttaagcgta tagggtttgt ccttcggttt
     1141 tgctcttttt atttggatgt cagatagcag catgtgtata gaaaaaagat cgaactcagt
     1201 tatacacaat gttatacgca aagatgtata gattccatta gaaataatgg tactttgctg
     1261 gacgagtata aggagatgat atcagttaaa acagtgagtt atggaattaa ttgaacttag
     1321 tgagaagtgt tcatggtgtc ccctgcagga atcgaacctg caattagccc ttaggagggg
     1381 ctcgttatat ccatttaact aagaggacaa tgcggcatga gtatacccgc taatggacta
     1441 cggggtaagt acacagccgc tcgattgctt aaaccctcgc catttatgcc gggtttttat
     1501 catttttctt aatgttttcc gcacgttctg ctttttgacg tgcttctgct ttgcgcttgt
     1561 tgctcatgtc gttacgaatc tgtgcatgac tcattaacgc gaagataaag gtgccgccgc
     1621 agatgttccc cgctaaagta ggtagtgcga agggccagat gaaatcgctc cagtgcagcg
     1681 tgccgttaaa caccagatag aggatttcaa cagaaccgac aacgatgtgg gtggtgtcac
     1741 ccagggcaat aagccaggtc atcaatataa tcaccacaat ctttgccgca cccgctgcag
     1801 gaaacatcca taccatagtg gcgatcagcc agccggaaat aatcgcgttg gcaaacatct
     1861 cgctgggggt gttcttcatc acatccatgc cgattttgac aaatgcatcg cgagtttctt
     1921 cattgaagat aggcatatat tcaaatgccc acgcagcaat acctgtcccg agaatattac
     1981 ccagcagcac gatgccccat aaccgcataa gtaagccgac gttgctcatt gtcggttttt
     2041 gcatgacggg tagtaccgca gttacggtgt tttcggtaaa taattgctgg cgggccatta
     2101 tgacgataat aaaaccaaag gtataaccga gattctccag caagaagctg cccggcacgc
     2161 cttccagttc gacatgaaat atcccttttg ccagtaacga agcgcccatc gacaggcccg
     2221 ccgcaatggc tgaccacagt agcgccattg cgtcgcgttc cagctctttt tcaccatcct
     2281 ggcggatatg ctcatgaatt gccatcgccc gggaggggag tcggtcttca tctatttcta
     2341 tttttttacc gcgctctttt tcttcgctct caacttcaat ttcgtcgctg tgttgatcaa
     2401 ttttgtcgtt gtccatggtc tcttctcttg aattagcacg tatagctaaa gcgtagcggc
     2461 ttttttgctc gcaactggcg ggagttactc tgaaaatgta gaaaaggccg cgtttgcctt
     2521 tttctgtttc tattgaatca agtagcctac agggcggcga ttaccaggct atgatcaaat
     2581 cagcaaatca gggcgtctgg acatcagttg acgtgctgtt acaatcgccc acacctaaac
     2641 aggcggatac ggtatcgttc cgtcatggat ggcaaactgc ataagccata aaaaaacagg
     2701 gagacattta tgaagcttcg cctgtcggcg cttgctctgg gaactacgct tctggtgggg
     2761 tgtgcgagtt ccggtacaga tcagcaaggg cgttctgacc cgttagaagg gtttaaccgc
     2821 actatgtaca acttcaactt caatgtatta gacccgtata ttgttcgacc ggtcgctgtc
     2881 gcctggcgtg attatgttcc gcaacctgcg cgtaacggtt tgagcaactt taccggcaac
     2941 cttgaagaac ctgcggtgat ggttaactac ttcttgcagg gcgaccctta tcaggggatg
     3001 gtccacttta cccgcttttt cctgaatacc attttgggaa tgggcggctt tattgatgtt
     3061 gcagggatgg cgaacccgaa actgcaacgg actgaacctc accgcttcgg tagtacgctt
     3121 ggtcattatg gtgtgggtta tgggccgtac gttcagttac cgttctacgg tagcttcacg
     3181 ctgcgtgatg acggtggtga tatggcggat agtctttacc cggttctttc ctggctgacc
     3241 tggccgatgt ctgtgggtaa atggacgctt gaagggatag aaactcgtgc gcagttgttg
     3301 gattccgacg gtctgctgcg tcagtcctcc gatccttata ttatggtgcg tgaagcgtac
     3361 ttccagcgtc atgatttcat cgctaatggc ggtgaactca aaccgcagga aaatccgaac
     3421 gcgcaagcga ttcaggatga tttaaaagat atcgattctg aataagaaac aaacaaaaaa
     3481 ggtgagtcgc aaaactcacc ttttttctta ttaagctaac gcgttatcag aacgcgtagt
     3541 taaagttagt accgaacagc caggctttac cttcagactc gaaggaataa gctgccgctc
     3601 cttcaccttc ggtgaatttc actttctgac catgcatata agatgcgcca acatcaatgg
     3661 atgcatcttc attaaatgcg taggttaacc cagcacttaa ccacaaacga tcctggtcag
     3721 gaatggaaat agaacgttta tctgccggaa ccgggctgtc gtcataagcg ataccggtac
     3781 ggaaggtcca gtttttatca taatagtagg ttgtacccaa cgcgatacga tacgcgtccc
     3841 ggaatccttc atctttatag aacagagtct gaccactatc gcctttcgct ttcaattcct
     3901 ggaactggct ccaactggta taggccatgc tgtagtgaat tgcccactgc ggcgcaacac
     3961 ggttataacc agaaatctcc cacatctcag gcaggttcag cgttaaggat ccattttggg
     4021 ttgcccccat cgtgccatac ggtaagccca tgttacccaa aatattgttc agatttgggt
     4081 taatggagct tttgtaatcc ccatcgaagt cgattttaac ttctgaacga tatgtcagcc
     4141 cccaacggtt atctttatcc agttcataaa gaatacccgc gttccagcca aagccccatt
     4201 cgtcaccttt caaatgagca atttgagagt ccggagtatt aataccattg gttaatgaag
     4261 cgggtacacc ctgggcaatc aactgttgag acaacaatgg ccccagcgaa ccggcgtaac
     4321 gctcaatttt tgccttggca taaactgcat caaaaccaag accaaaactc cagctattgt
     4381 taaggcgata agccccgcta aggttaaggt tgattgtttg cagatcggtt ttaccgccat
     4441 agataccagc agcgtagttg tcggtaaatt ccgtagccag gccatagttg gaagttattg
     4501 acgctcccca accaaactga tcgttaatag gcgcgacaaa gtgcaggtta ggtacccacg
     4561 ctgtcggcgc gatattttta gcatttgcac tctgaccggt taaattagaa tgtccggtaa
     4621 tatctacatc cggatcaata taaaccgcac ccgcagaaaa tgtcgggcgg tcaaacatgg
     4681 taatcaatgc ggggttacgg ctaacgttac ctgcatcatc ggcaattgca ccttcccctg
     4741 aataagcccg gcccaggcca gaggaagaaa attcgtttaa ctgaaagcct gccgaccagg
     4801 cctgggtgga gataagtgcc actgcgactg cgagagcaga ctttgtaaac agggttttct
     4861 ggctcatgac cataacctca atgatttatt tttatacaaa ctatgttacg tgctgtaaca
     4921 ggagcgcgaa gtgtagggtc tgaggtacat cttagaaatc agaccagtgg cgagagtata
     4981 ggtcggacca gctgggatgt tgcaagtatg tttctatctt ttttcagata tgatctaaga
     5041 aacgcgatcc agctggcaaa attgggaggt ggcgcactca tggattcggg tgaattttgt
     5101 tttagatcat ttttaagtgt gatttcggtc acttatccga ttttgcagaa ttaacaactg
     5161 gagcagcact ttccggaagc gtaaaatatc cacaacgttt atctggcacc ttgggtgcac
     5221 atacagagga aatcaacgat gagtaaatgt agtgctgatg aaaccccggt ttgctgctgt
     5281 atggatgttg gcaccattat ggacaactcc gactgcaccg cgtcttacag ccgtgtgttt
     5341 gcaaaccgcg cagaagcaga acaaacgctg gcggcgctga ctgaaaaagc ccgtagcgtg
     5401 gaatccgaac cgtgcaaaat caccccgact ttcactgaag agtctgacgg tgtgcgcctg
     5461 gatatcgatt tcacttttgc ctgcgaagct gaaatgctga tcttccagct gggtctgcgt
     5521 taattcctct cgggtacata cgcccctgtc agctgctgac aggggcgttt tattttctcc
     5581 tcctcttttc tctctgtgtc atagctccca ctttcccttt tctccacttg gctaaatgta
     5641 aaaaaatggt taagactatg atcaggtcag accactttat ttattttttt acaggggagt
     5701 gttatgggtc aggttttacc gctggttacc cgccagggcg atcgtatcgc cattgttagc
     5761 ggtttacgta cgccttttgc ccgacaggcg acggcttttc atggcattcc cgcggttgat
     5821 ttagggaaga tggtggtagg cgaactgctg gcacgcaccg agatccccgc tgaagtgatt
     5881 gaacaattgg tcttcggtca ggttgtgcaa atgccggaag cgcccaacat tgcccgtgaa
     5941 attgttcttg gcactggaat gaatgtgcat actgatgctt acagcgtcag ccgcgcttgc
     6001 gccaccagtt ttcaggcggt tgcgaacgtt gccgaaagcc tgatggcggg aactattcga
     6061 gcggggattg ccggtggggc agattcctct tccgtattgc caattggcgt cagtaaaaaa
     6121 ctggcgcgcg tactggttga tgtcaacaaa gcccgcacaa tgagccagcg cctgaaactc
     6181 ttctctcgcc tgcgtttgcg cgacttaatg cccgtaccgc ctgcggtagc agaatattct
     6241 accggcttgc ggatggggga caccgcagag caaatggcga aaacctacgg catcacccga
     6301 gaacagcaag atgcactggc gcaccgttcg catcagcgtg ccgctcaggc gtggtcagaa
     6361 ggtaaactca aagaagaggt gatgactgcc tttatccctc cttataaaca accgcttgtc
     6421 gaagacaaca atattcgcgg taattcctct cttgctgatt acgcaaagtt gcgtccggcg
     6481 tttgatcgta aacacgggac ggtaacggcg gcgaacagta cgccgctgac cgatggcgcg
     6541 gcagcggtga tcctgatgac tgaatcccgg gcgaaagaat tagggctggt accgctgggc
     6601 tatctgcgca gctacgcatt tactgcgata gatgtctggc aggacatgtt gctcggtcca
     6661 gcctggtcaa caccgctggc gctggaacgt gccggattga cgatgggcga tctgacattg
     6721 atcgatatgc acgaagcctt tgccgcccag acgctggcca atattcagtt gctgggtagc
     6781 gaacgttttg ctcgcgacgt actggggcgt gcacatgcca ctggcgaagt ggacgagagc
     6841 aaatttaacg tgcttggcgg ttcgattgct tacgggcatc ccttcgcagc gaccggcgcg
     6901 cggatgatta cccagacatt gcatgaactt cgccgtcgtg gcggtggatt tggtttagtg
     6961 accgcctgtg ctgcgggtgg gcttggcgca gcaatggttc tggaggcgga ataatggaaa
     7021 tggcatcagc gtttactctt aatgttcgtc tggacaacat tgctatcatc accatcgacg
     7081 taccgggtga gaaaatgaat accctgaagg cggaatttgc ctcgcaggta cgcgccatta
     7141 ttaagcaact ccgtgaaaac aaagagttgc gtggcgtggt gtttgtctcc gcgaaaccag
     7201 acaacttcat tgctggcgca gacatcaaca tgatcggcaa ctgcaaaacg gcgcaagaag
     7261 cggaagtact ggcgcggcag gggcaacagt taatggcgga gattcatgct ttgcccattc
     7321 cggttatcgc ggctattcat ggcgcttgcc tgggcggcgg gctggagttg gcgctggcgt
     7381 gccacggtcg tgtttgtact gacgatccta aaacggtgct cggtttgcct gaagtacaac
     7441 ttggattgtt acccggttca ggaggtaccc agcgtttacc gcgcctgata ggtgtcagca
     7501 cagcattaga gatgatcctc accggaaaac aacttcgggc gaaacaggcg gtaaagctgg
     7561 ggttggtgga tgacgtcgtt ccgcactcca tattgctgga agccgctgtt gagctggcaa
     7621 agcaggatcg cccatcttca cgccctctac ctgttcgcga gcgtattctg gcggggccgt
     7681 taggtcgtgc gctgctgttc aaaatggtcg gcaagaaaac agaacacaaa acgcaaggta
     7741 actatccggc gacagaacgc atcctggagg ttgtcgaaac gggattagcg caggggacca
     7801 gcagtggcta tgacgccgaa gctcgggcgt ttggtgaact ggcgatgacg ccgcaatcgc
     7861 aggcgctgcg caatatcttt tttgccagta cggacgtgaa aaaagatccc ggcagtgatg
     7921 cgccgcctgc gccattaaac agcgtgggga ttctaggtgg tggcttgatg ggcggcggta
     7981 ttgcttatgt cactgcttgt aaagcggggc ttccggtcag aattaaagat atcaacccgc
     8041 agggcataaa tcatgcgctg aagttcagtt gggatcagct tgagggcaaa gttcgccgtc
     8101 gtcatctcaa agccagcgaa cgtgacaaac agctggcatt aatctccgga acgacggact
     8161 attgcggctt tgcccatcgc gatctgatta ttgaagcggt gtttgaaaat ctcgaattaa
     8221 aacaacagat ggtggcggaa gtggagcaaa attgcgctac tcataccatc tttgcttcga
     8281 acacgtcatc tttaccgatt ggcgatatcg ccgctcacgc cacgcgacct gagcaagtta
     8341 ttggcctgca tttcttcagt ccggtggaaa aaatgccgct ggtggagatt attcctcatg
     8401 cgagtacatc ggcgcaaacc atcgccacca cggtaaaact ggcgaaaaaa cagggcaaaa
     8461 tgccaattgt cgtgcgtgac aaagctgggt tttacgtcaa tcgcatctta gcgccttaca
     8521 tcaatgaagc tatccgcatg ttgaccgaag gcgagcgtat tgagcatatc gatgccgcgc
     8581 tagtgaaatt tggttttccg gtaggcccaa tccaactttt ggatgaggta ggaatcgaca
     8641 cagggactaa aattatgcct gtactggaag ccgcttatgg agaacgtttt agcgcgtctg
     8701 caaatgttgt ttcttcaatt ttgaacgacg atcgcaaagg cagaaaaaat ggtcggggtt
     8761 tctatcttta tggtcagaaa gggcgtaaaa gcaaaaaaca ggtcgatccc gccatttacc
     8821 cgctcattgg cgtacaaggg caggggcgac tctccgcacc gcaggttgct gaacggtgtg
     8881 tgatgttgat gctgaatgaa gcagtacgtt gtctggacga gcaggttatc cgtagcgtgc
     8941 gtgacgggga tattggtgcg gtatttggca ttggttttcc gccatttctc ggtggaccgt
     9001 tccgctatat cgattctctc ggcgcaggcg aagtggttgc aataatgcaa cgactggcca
     9061 cgcagtatgg ttcccgtttt accccttgcg atcgtttagt tgagatgagc gagcgtgggg
     9121 aaagtttttg gaaaacaact gcaactgacc tgcaataaga aggtcaaagt tatatgaatc
     9181 cgcgctgaat ggcggagtgt tgctcaaaat gtaaacgcat attgactata cttacgccat
     9241 tgaggtaaaa aacagcgttt cattcggtga atggataagg cacaatgccg gccaccgttt
     9301 tctttctctg gtttcagatg aaagaaaacg ggcgaatctg gttaacaaaa gcggtgcaat
     9361 atgcaagttt ttatcatgcg tcacggcgac gcagccctcg atgccgccag tgattcggtt
     9421 cgtcctctga ccactcatgg ttgtgacgaa tctcgcctga tggcgaactg gctgaaaggt
     9481 caaaaagtgg aaatcgaacg cgttctggtg agcccgttcc tgcgagccga gcaaacactt
     9541 gaagaagtag gggattgtct gaacctgcct tccagtgcag aagttctgcc ggagttaacg
     9601 ccctgcggcg atgttggtct ggtcggtgcc tatttgcagg cgttgaccaa tgaaggtgtt
     9661 gcttcggtac tggtgatctc ccacttaccg ttagttgggt atctggttgc cgagttatgt
     9721 ccgggcgaaa cgccgcctat gtttaccacg tcagcgattg ccagcgtaac gcttgatgaa
     9781 agcggaaaag gccaattcaa ctggcagatg agcccttgta atttgaagat ggcaaaagca
     9841 atttaagcaa tcaatgcccg gttacgtgcg cgtacaccgg gcagtttctc cgtcatacaa
     9901 tcattctttt tttgtctaaa tgttgttaat atttaacaac tctcgatagt tattttc
//
LOCUS       DACADF010000156         9914 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.158, whole genome shotgun sequence.
ACCESSION   DACADF010000156 DACADF010000000
VERSION     DACADF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.158"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(189..380)
                     /locus_tag="ISB30_003966"
     CDS             complement(189..380)
                     /locus_tag="ISB30_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HAP1991549.1"
                     /translation="MGINAVVMDGVVIGENSIVGVSAFVKAKAEMPTNYLIVSSPAKA
                     IRELSEQELALRKQGIRCW"
     gene            397..666
                     /locus_tag="ISB30_003967"
                     /pseudo
     CDS             397..666
                     /locus_tag="ISB30_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(838..1164)
                     /locus_tag="ISB30_003968"
     CDS             complement(838..1164)
                     /locus_tag="ISB30_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAP1991550.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(1172..1357)
                     /locus_tag="ISB30_003969"
     CDS             complement(1172..1357)
                     /locus_tag="ISB30_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAP1991551.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1354..3993)
                     /locus_tag="ISB30_003970"
     CDS             complement(1354..3993)
                     /locus_tag="ISB30_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAP1991552.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWGRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLAPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEDKQ"
     gene            4201..5190
                     /gene="ldhA"
                     /locus_tag="ISB30_003971"
     CDS             4201..5190
                     /gene="ldhA"
                     /locus_tag="ISB30_003971"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAP1991553.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5301..5723
                     /gene="hslJ"
                     /locus_tag="ISB30_003972"
     CDS             5301..5723
                     /gene="hslJ"
                     /locus_tag="ISB30_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAP1991554.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5720..5986)
                     /locus_tag="ISB30_003973"
     CDS             complement(5720..5986)
                     /locus_tag="ISB30_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAP1991555.1"
                     /translation="MRAAFWVGCAALLLSACSREPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            6260..9784
                     /gene="nifJ"
                     /locus_tag="ISB30_003974"
     CDS             6260..9784
                     /gene="nifJ"
                     /locus_tag="ISB30_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP1991556.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTSEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPTELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2489 a         2576 c         2412 g         2437 t
ORIGIN      
        1 acggtacaaa tcaatactca aataaataaa tcagagcaga agcataattc tgctttataa
       61 ttaaagggaa aaataattat ataactgtta ctgtttcggt cgcagattct catcaaacac
      121 caggcgtttc ctgccaggtt caacttcccg caatggctcg acttgatgca acgtctgctt
      181 acaacgagtc accagcacct gatgccctgc tttctcaatg ccagctcctg ctcgctgagt
      241 tcacgaatcg ctttcgccgg gctgctgaca atcaagtaat tagtcggcat ttctgctttg
      301 gctttcacaa atgcggaaac accaacaata ctgttctcac caatcaccac accgtccatc
      361 actaccgcgt taattcccac taatgtatgg cggtggttaa tgcagccgtg aagaatagtc
      421 ggagatacag gtatgtcagt acgtagtttg taccgaatgt ttgccgataa aggtttggtg
      481 gtcgcgcaat atattcgtaa ccgtcgtctc gatttttgtg cagatgcgat tcgccatgcc
      541 gcagatgatg aaaaactggc aggcattggc tttcattggg gattttccga ccagagtcat
      601 ttttcaacgg tatttaaaca acgctttggg atgacgccag gcgagaatcg gcgtaaattc
      661 cgctaactaa ttcgcgttgc atgtacagca acgcgaatta gttaaaacag atatttgact
      721 atgcattttt cagggcgaaa ggcaatttga caggcgtttt tgaataatca tcttgtttcc
      781 ggaaaataga ttaatcgcct tctcctgtag cgttatcccg atattgcggc tttactatta
      841 aaattttcgc acccatttac cgtttatccc ttgtacatat tccccgggtt tggcccgttc
      901 tacgagcttt tgcccggcga gtttcgcgat atcatctacc gacacattat tttgctttgc
      961 cagttgttga tagctcgctt tacgttcggc attaatgtcg gttactaatt tctcagtttc
     1021 agcatctgtt ttcagcgcaa ccagataacc ataaaatgtt tcgcctaccc gcccctgagt
     1081 tctggcttca tccagagtca atgccattac attgctgctt accaagccaa caagaaatgc
     1141 acaaagaagt aatgttcttt tcatcatcac ctcaaaaaag atcgctacgg gtctcaagca
     1201 gctcttcgac atctttgtct gccttgatga tgatctcatg ctcaatttta acgttcatat
     1261 tgatagtgat cggttcctta ggtgcagcga cttcaatacg aggcgtacag ccaaccagca
     1321 taaaagatga cgtcaacgca gccagtaaaa ttttcattgt ttatcctcac actcttttcc
     1381 ttgcggacag tcatttcccg gcagacgcgc gttctgctcc agccatgcct ggagattatc
     1441 gccaaagcgt aaactgcgcc acagcgtaaa aatattctct tcatgatggt aattaagatt
     1501 taccgtaccg cttttaccat caacgcgact ggtacctgta atattggcct gcatggttaa
     1561 taaaccgaga ttatctaaat taatttttgt cgatgaacgg ctaatttcca tatagcgcaa
     1621 ccagttaatt gctgaacccg cggtcatatt gtctttcacc accgcatctg cggtgtcttt
     1681 atccagccgc aaggtcattg gtccgctatt tgccagccag ccgtctttca ctatccattt
     1741 ttcattgttt aaccataacg gcagtgcacc actaaatgcc ccggacatgg cgaattgttt
     1801 cggattaacg gcgctaacca gttcgctgga tgagagatta ttcagtcgca acagtgccgg
     1861 gtcatgttgc ggcatacgta attgtttcat cagcacgtta ccgcctaaca catcgacact
     1921 aacatcagtc aacagcaatg gttcttcttc agtccacgga taacgccctt gcaaatcagc
     1981 cgtaatattt ttcgctgtca ccagattaat cacttcggca attcgcaacg taacggggcc
     2041 gcgagtcccc agatgccagg ctccatcggc aaaacggaaa ggcaggacaa aatcgacacc
     2101 gttaacctga ttatctggca tccaggcact accgcctttc aatacgccgt gcccccccgc
     2161 gcggaatcct tgttcgggcg ctgctgaaaa tgcgacctga gcatacagtt caccatcgcg
     2221 taagttcatc ttccagtcgg gtggcgccaa cggctggaat acagtcagtg attgtttagg
     2281 ccaccaggcg ttaccgcgca gacgaatacc gtcccagcga ccatttaccc gaaccgggcc
     2341 aatctcacca gcatgtaaat cgcctttaaa gaggaaatac gtcggatcgc gcccatcgac
     2401 actaaatttt aaggttgatg gcggtaacac actgccgcca gtgaacagcg tttgcccggc
     2461 gtccagtgac agagcgccgc tgaaggaggg atgctgtgcg tcacgtcccc agacgacggg
     2521 cttgtcgaga attaatcgcg gcttttctac cgtcatcgta ccgtattgta actggtcgaa
     2581 accggtggag agatcggtca gcgtaatcgt gctgtcatgc cactcacctt ttcctgcgac
     2641 atcccaggtg gcattcatcg gtgtaaaact cccttttccc cagtagcgcc actgccagcg
     2701 gccagcgtca gggagaaaat cattcgccag cccatccata tgcagcacga aatcgcccag
     2761 ttcattttca tgcgcctgca aaatggcctg caaacgtccg tcaacaccac gttgggtgac
     2821 ttttacaccc gctaaaggcc agcggatttc atcgatgtcc agcgaatcaa tgactcttcc
     2881 ctttgaacga agtaacgcgc caggctcaaa ggtcagcgtt gggtcagaca gacttccact
     2941 tagctgcgca ggtaaacggg catataaaat gaggtccgcc tgtttcgctt caccggtcag
     3001 ctgcagaggc agctggctgt tatccatgct taattttcct gggccaaaat ttaacaccgc
     3061 gttgccttta cccgcttgcc cctgggtcag cacactcagt cgcccgctga ccagagcgtt
     3121 ctcgagccct gcctgccagt tgtcgacttt gacacccagt cggccactca aaggaaaacc
     3181 tgcatacggc cagctccagc gaccatcgct tacggtcaat tgttgacgag taatttgcca
     3241 cggcaaatcg agcaacggat cgccgttatc ccgtgccagc acaatcaatt gcccgctatt
     3301 ttcctgccag tccagctcgg catccaccag tgaaggctcc tgcggtaagt ttaacgtcgc
     3361 agtagcatga ccacttacag gaagtccatc cggcacgagc ggcatagtaa attcccccac
     3421 cagttttacc ggcggctgat tttcaaacgc gacgacatcc agttcgctga ctgtaagttg
     3481 ttgcccttta agctggcctt gaaatttaac tttttcgccc tgataacgca gttgctggat
     3541 atcggaggtt aatgcgagag agagttttcc ctgccattcc tgccagggag aaaaaatcag
     3601 tttatcgata ttgatccagg tgttaggcaa catggcctgc cactgcgcga gggttttggg
     3661 agcggctggc gattgctccg tctgcggcaa tttcgccaga caagcagaat caagttctac
     3721 cgtgccgacg ttcaataacc agcggctggg atgtgaaagg ctggcgttgg tgatatgcgc
     3781 aagctgacaa tctcccacca gataacggag atcggggatg attaaacctt tacgcgtaat
     3841 gcgtgggctt tcatctaatg caatacgtgt cccgagcggt agccagatgc ccgccagcgt
     3901 gggaacccac agcccgagcg tcatcagcag cgtcaacggc acaagaataa tcagtaataa
     3961 cagcgcgaga acggctttat atttacccag catgggtagt taatatcctg atttagcgaa
     4021 aaattaagca ttcaatacgg gtattgtggc atgtttaacc gttcagttga aggttgcacc
     4081 tacactaagc atagttgttg atgaattttt caatatcgcc ataactttca attatatttg
     4141 aaattttgta aaatattttt agtagcttaa atgtgattca acatcactgg agaaagtctt
     4201 atgaaactcg ccgtttatag cacaaaacag tacgacaaga agtacctgca acaggtgaac
     4261 gagtcctttg gctttgagct ggaatttttt gactttctgc tgacggaaaa aaccgctaaa
     4321 actgccaatg gctgcgaagc ggtatgtatt ttcgtaaacg atgacggcag ccgcccggtg
     4381 ctggaagagc tgaaaaagca cggcgttaaa tatatcgccc tgcgctgtgc cggtttcaat
     4441 aacgtcgacc ttgacgcggc aaaagaactg gggctcaaag tagtccgtgt tccagcctat
     4501 gatccagagg ccgtagctga acacgccatc ggtatgatga tgacgctgaa ccgccgtatt
     4561 caccgcgcgt atcagcgtac ccgtgacgct aacttctctc tggaaggtct gaccggcttt
     4621 actatgtatg gcaaaacggc aggcgtgatc ggtaccggta aaatcggtgt ggcgatgctg
     4681 cgcattctga aaggttttgg tatgcgtctg ctggcgttcg atccgtatcc aagtgcggcg
     4741 gcgctggaac tcggtgtgga gtatgtcgat ctgccaaccc tgttctctga atcagacgtt
     4801 atctctctgc actgcccgct gacaccggaa aactaccatc tgttgaacga agccgccttc
     4861 gatcagatga aaaatggcgt gatgatcgtc aataccagtc gcggtgcatt gattgattct
     4921 caggcggcaa ttgaagcgct gaaaaatcag aaaattggtt cgttgggtat ggacgtgtat
     4981 gagaacgaac gcgatctgtt ctttgaagat aaatccaacg acgtgatcca ggatgacgta
     5041 ttccgtcgct tgtctgcctg ccacaacgtg ctgtttaccg ggcaccaggc attcctgaca
     5101 gcagaagctc tgaccagtat ttctcagact acgctgcaaa acttaagcaa tctggaaaaa
     5161 ggcgaaacct gcccgaacga actggtttaa tcttgccgct cccctgcatt ccaggggagc
     5221 ttattcagat aatccccaaa gacctttcat cctctattct taaaatagtc ctgaatcaga
     5281 aacagtaatt gagaaccaca atgaagaaag tagccgcgct tgttgcgcta agcctgctga
     5341 tggcgggatg tgtaagtagt gacaaaattg ctgtaacgcc agaacagcta cagcatcatc
     5401 gctttgtgct ggaaagcgta aacggtaagc ccgtgaccag cgataaaaat ccgccagaaa
     5461 tcagctttgg tgaaaagatg atgatttccg gcagcatgtg taaccgcttt agcggtgaag
     5521 gcaaactgtc taatggtgaa ctgaccgcca aagggctggc aatgacccgt atgatgtgcg
     5581 ctaacccgca gcttaatgaa ctcgataaca ccattagcga aatgctgaaa gaaggtgcac
     5641 aagtggatct gaccgcgaac cagttaacgc tggcgaccgc gaaacagaca ttaacttata
     5701 agctggcgga tttaatgaat taatagctgc cacagctccc ggcggcaagt gactgttcgc
     5761 tacagcgttt gccgttgggt aatgcacaca tcccaatcgc cgtaccatcc agttgacggg
     5821 caacagaaag cgaaccgccg atcattgcac aatttgcttc tccactactg gacatcgacg
     5881 cttttaaacc tggcgctacg tgcgccgcag tcgcctgctg aacaggttca cgactacacg
     5941 ccgacaacaa taaagcggca caccctaccc aaaacgctgc tcgcatcttt tcttcctctg
     6001 atcttcaagc caaacgacac cgccataaat aataggcagc acagagggcg gcgtcgagag
     6061 ctgtcctgcg cgttgccccg catttttact tttttatggc tatttttttg ccctctgttt
     6121 gatcaaaaca ttcattacgc tgatgtgggg gacacaaaag cgaaaatgca gaagaaagcc
     6181 atttgctaaa attgaacgat tactactggg cgcgcagcaa tttcgtgcgc ccctcatttg
     6241 cgcaatgtaa gggtgtcata tgattactat tgacggtaat ggcgcggttg cttcggtggc
     6301 gtttcgcacc agtgaagtta tcgccatcta ccctattacc cccagttcca cgatggcaga
     6361 acaggctgat gcatgggccg gaaacggctt gaaaaacgtt tggggagaca caccacgcgt
     6421 ggttgaaatg cagtcggaag cgggtgctat cgctaccgtg catggtgctt tgcaaactgg
     6481 tgccctctca acatcgttta cgtcatcgca gggtttgctg ctgatgatcc caacgctgta
     6541 caaactggca ggcgaactca caccgtttgt cctgcatgtt gcggcacgta ccgttgccac
     6601 acatgcactc tctatttttg gcgatcattc cgacgttatg gcagtgcgcc aaacgggttg
     6661 cgcgatgttg tgtgcagcaa acgtccagga agcgcaagac tttgctctca tttcgcacat
     6721 tgcgacgctg aaaagtcgcg tgccatttat tcatttcttt gatggtttcc gcacatctca
     6781 cgaaatcaat aaaattgtcc cgctggccga tgacacgatt cttgaactga tgccgcaggc
     6841 agaaattgat gctcatcgcg cccgggcact caacccggaa catccggtga tccgcggtac
     6901 gtccgccaat cctgacactt atttccagtc tcgcgaagcc accaacccat ggtacaacgc
     6961 ggtctatgac catgttgaac aggcgatgaa tgatttcgct gccgcgacag gtcgtcagta
     7021 tcagccgttt gagtattacg ggcatccgca agcggaacgg gtgattatcc tgatgggctc
     7081 tgccattggc accagtgaag aagtggttga tgaattgctg acccgtggcg aaaaagtcgg
     7141 cgtgctgaaa gttcgcctgt accgcccctt ctccgccaaa cacttgctgc aagctctgcc
     7201 gggatccgtg cgcaacgtgg cggtactgga cagaaccaaa gaacccggtg cccaggcaga
     7261 accgctctat ctggatgtga tgactgcact ggcagaagcc tttaataatg gcgagcgcga
     7321 aaccctgccc cgtgtcattg gtgggcgcta tggtctttca tccaaagaat ttggcccgga
     7381 ctgtgtactg gcggtatttg ccgagctcaa cgcggctaaa ccgaaagcgc gctttacggt
     7441 tggtatttac gatgatgtaa ccaatctgtc actgccgttg ccggaaaaca ccctaccaaa
     7501 ctcggcgaaa ctggaagcct tgttttatgg ccttggtagt gatggcagcg tttccgcgac
     7561 caaaaacaat atcaagatta tcggaaattc cacgccgtgg tacgcacagg gctattttgt
     7621 ttacgactcc aaaaaggcgg gcggcctgac ggtttctcac cttcgcgtga gcgaacagcc
     7681 gattcgttcc gcttatctca tttcccaggc tgattttgtt ggctgccacc agttgcagtt
     7741 tatcgataaa tatcagatgg ctgagcgttt aaaacctggc ggcattttcc tgctcaacac
     7801 gccgtacagc gcagctgaag tgtggtcgcg cttgccgcag gaagttcagg ccgtgctaaa
     7861 ccagaaaaaa gcgcgcttct atgtgattaa cgcggcgaaa atcgcccgcg aatgtggcct
     7921 ggcggcccgt attaataccg tcatgcagat ggcttttttc catctgacgc agattctgcc
     7981 tggcgatagt gccctcgcag aattgcaggg tgcgattgcc aaaagttaca gtagcaaagg
     8041 ccaggatctg gtggaacgca actggcaggc tctggcgctg gcacgtgaat ccgtagaaga
     8101 agttccactg cagccagtta atccgcatag cgccaatcga ccgccggtgg tttccgatgc
     8161 cgctcctgat ttcgtgaaaa ccgtaaccgc tgcgatgctc gccgggcttg gcgacgccct
     8221 ccccgtttcg gcgctgccgc cagacggcac ctggccgatg ggcaccacgc gctgggaaaa
     8281 acgcaatatc gccgaagaga tccccatctg gaaagaggaa ctctgtaccc aatgtaacca
     8341 ctgtgtcgcc gcttgcccac actcagctat tcgcgcaaaa gtggtaccgc ctgaagcgat
     8401 ggaaaacgcc cctgccagcc tgcattcgct ggatgtgaaa tcgcgtgata tgcgcgggca
     8461 gaaatatgtc ttgcaggtgg ctccggaaga ttgcaccggt tgtaacctgt gcgtcgaagt
     8521 ttgcccggcg aaagaccgtc agaatccaga gattaaagcc atcaatatga tgtcgcgcct
     8581 ggaacatgtc gaagaagaga aaatcaatta cgatttcttc ctcaacctgc cagaaatcga
     8641 tcgtagcaaa ctggaacgta ttgatattcg tacatcgcag ctaatcacgc cgctgtttga
     8701 atattccggt gcttgctccg gttgtggcga gacgccgtat attaaattgc tgactcagct
     8761 ctatggcgac cggatgttga tcgctaatgc cactggctgt tcttcaatat acggcggtaa
     8821 cctgccttca acaccatata ccaccgatgc caacggtcgt ggaccggcat gggcgaactc
     8881 gctgtttgaa gataacgccg aatttggcct tggtttccgc ctgacggtcg atcaacaccg
     8941 tgtccgcgtg ctgcgtctgc tcgatcaatt tgccgataaa atcccgacgg aattactgac
     9001 tgcgttgaaa tcagacgcca cgccagaggt tcgtcgtgaa caggtagcag ctttacgcca
     9061 gcaactcaac gatgttgccg aagcacatga actgctgcgt gacgcagatg cactggtgga
     9121 aaaatcaatc tggctgattg gtggcgatgg ctgggcttac gatatcggct ttggcggtct
     9181 ggatcatgta ttgagtttga cggaaaacgt caacattctg gtgctggata cgcaatgcta
     9241 ttccaacacc ggaggtcagg catcgaaagc gacaccgctg ggtgcagtaa ctaagtttgg
     9301 cgagcatggc aaacgtaaag cgcgtaaaga tcttggcgtc agtatgatga tgtacggtca
     9361 tgtttatgtg gcgcagattt ctctcggcgc gcagctgaac cagacggtga aagcgattca
     9421 ggaagcggaa gcgtatccgg ggccatcgct gatcatcgct tatagcccgt gtgaagagca
     9481 tggttacgat ctggcactca gccacgatca gatgcgccaa ctcactgcta ccggcttctg
     9541 gccgttatat cgcttcgatc ctcgtcgtgc cgatgaaggc aaactgccgc tggcgctgga
     9601 ttcacgcccg ccgtcagaag cacttgaaga aacgttactt cacgagcagc gtttccgtcg
     9661 tctgaattcg cagcagccag aagtggcaga acagttatgg aaagatgctg cagctgattt
     9721 gcaaaaacgc tatgacttcc tggcacaaat ggccggaaaa gcggaaaaaa gcaacaccga
     9781 ttaaatgctc tggataagga ttatccaatt caaaaaaaaa gcccggacga ctgttcgggc
     9841 ttgtcttctt atgtgttgga aaatcagtag caatgcaatg gcccaacaga aaataaaatc
     9901 tgtgacaata aagg
//
LOCUS       DACADF010000157         9849 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.159, whole genome shotgun sequence.
ACCESSION   DACADF010000157 DACADF010000000
VERSION     DACADF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.159"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..409)
                     /gene="folK"
                     /locus_tag="ISB30_003975"
     CDS             complement(<1..409)
                     /gene="folK"
                     /locus_tag="ISB30_003975"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAP1991557.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPEL"
     gene            complement(406..1824)
                     /gene="pcnB"
                     /locus_tag="ISB30_003976"
     CDS             complement(406..1824)
                     /gene="pcnB"
                     /locus_tag="ISB30_003976"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAP1991558.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(1863..2789)
                     /gene="gluQRS"
                     /locus_tag="ISB30_003977"
     CDS             complement(1863..2789)
                     /gene="gluQRS"
                     /locus_tag="ISB30_003977"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAP1991559.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQNIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2826..3281)
                     /locus_tag="ISB30_003978"
     CDS             complement(2826..3281)
                     /locus_tag="ISB30_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAP1991560.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3459..4163)
                     /gene="sfsA"
                     /locus_tag="ISB30_003979"
     CDS             complement(3459..4163)
                     /gene="sfsA"
                     /locus_tag="ISB30_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP1991561.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4178..4708)
                     /gene="thpR"
                     /locus_tag="ISB30_003980"
     CDS             complement(4178..4708)
                     /gene="thpR"
                     /locus_tag="ISB30_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAP1991562.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4782..7211
                     /gene="hrpB"
                     /locus_tag="ISB30_003981"
     CDS             4782..7211
                     /gene="hrpB"
                     /locus_tag="ISB30_003981"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAP1991563.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTQMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLVTQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSCLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFLRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPRLAGAYADRIARRRGQEG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSSSPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEASN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7305..9830
                     /gene="mrcB"
                     /locus_tag="ISB30_003982"
     CDS             7305..9830
                     /gene="mrcB"
                     /locus_tag="ISB30_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAP1991564.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQQPAQQEQKDSDGVAGWIKEMFGSN"
BASE COUNT         2257 a         2516 c         2815 g         2261 t
ORIGIN      
        1 ccagctccgg cgcaatttca aacagtggcc acagcataaa gccacgattt ttcatatcgt
       61 agtgcggaac ggtcagacgt tcggtattta ttacttcatt accaaacagc atgatgtcga
      121 gatccagcgt gcgcggtccc cagcgttcag ctttacggac gcgaccttgc tgcaattcga
      181 tacgctgtgt gtgattgagt agctcttcag gggcaagagt ggttttcagc gcgacggcgg
      241 catttaagta atcgggttga tcttgcggcc ccagaggtgg ggtgcggtaa aacgaagaaa
      301 cggcaagaat gcggctttct ggaatatcgc ctaatgcttt cagggcagca ttgacctgct
      361 ccagcggaga ggccagattg ctgcctatgg caatatacgc cactgtcatg tagtaccctc
      421 acgacgcggt gcgcgtttgc gtggacgacg agtacgacga cgcggtgacg gttcttcatc
      481 cagttcgtta agcatccctt tttggtctgg cggcgcggaa acctggaact caccccacca
      541 tttcaccaga cgttgcagtt cagcgttacg ctcaacttcg gctcgtaagg ccaacaggtc
      601 gtatgccgca cggaacttag gatgctccag cagtttccat gcgcgtttac cctgacgacg
      661 ggacatacgc aactgcaact gccagatatc gcgagttagc gtagtcagac gtttcgggat
      721 cgccagtgaa cggcaggctt cgtccagcac gtcgttcatc gccagtgcga aagcgtcgtg
      781 ataggtcagg ccgctctcct gagcaatttt ctgtgccgtc tccagcaatg ggtaccagaa
      841 catggcggca aacaggaacg ccgggttcac gcgcatatcg ttatggatac gcgtatcggt
      901 attcttcagt acctgctcaa taatgcgttc catcgggctg tcgccatttt ccgtgaagta
      961 gcgggtaatg gtcgggaaca gcggctggaa cagatgatat tcacacagca gcttataggt
     1021 ttcgtaaccg tagcccgctt gcaacagttt cagcgattct tcaaacagac gtgccggtgg
     1081 gatatcgttc agtaaggttg ccaggcgtgg gatcggctcg gcggtttccg ggctgatgcg
     1141 catgcccaat ttggcggcaa aacgaaccgc gcggagcata cgtaccggat cttcacggta
     1201 gcgcgtttcc gggttaccaa tcagacggat aacgccgtct ttcagatcct tcatgccgcc
     1261 aacgtaatca cggacggtaa aatctgccac gctgtaatac aggctgttga tagtgaaatc
     1321 gcggcgctgg gcgtcttctt cgatggagcc gaaaatgttg tcgcgcagca acataccgtt
     1381 ttgcccgcgt tgggaggtcg tgcggtcgct gacgttacct tcgtgatgtc cacggaaggt
     1441 cgcaacttcg ataatctccg ggccaaacat cacatgagcc agacggaaac ggcgacccac
     1501 cagacggcag ttacggaaca gtttacgtac ctgttcaggc gtggcgttag tggttacgtc
     1561 aaaatctttc ggctttttgc caagtaacag gtcgcgcacg ccgccgccaa ccagccaggc
     1621 ttcgtatccc gctttattga gcctgtacat taccttcagg gcattttcac tgatatcttt
     1681 gcgggaaata gcatgctgct cacgcgggat caccgtcacc tgtggacggg cgactgcctg
     1741 ttcagcctcg ctttcctcgc ggcttagcac cttgcggcaa aaattagcga ctcgggtaaa
     1801 aatagtacac ctcggtagtg tcaaacatca ttcaggacaa aaaaatagcg gctaatcata
     1861 gctcagcatg acgcatttga gaatgttgaa tttacaattg ccgactcagg cactgcggtg
     1921 agccgccagt ttttgacggc tgactgaagg atttgctcga cgctgaaatc ctgccagtgt
     1981 gtttctacct gctgacccag aaaatgaagt gccgcgatta acaccgggcg cggatcgcct
     2041 ttcggcaacg caggcgcatg attctgcttg gaaagtttag cgccttgtgg attaagcgcc
     2101 agcggtaaat ggatgtaatc tggcactttc cagccaaaaa gctggtacag cgagatttgc
     2161 cgcacagtcg gttcgatcaa atctgccccg cggacaattt cgctcacgcc ctggaagtga
     2221 tcatccacca ccaccgccag gttataggca aacaaaccat cacggcgatg aataataaaa
     2281 tcttcccgtg ccagtttttc gtcggcgtga ataatgcctc gcagcaggtc agtaaactgg
     2341 gtgactggat gctgctggcg gatacgtact gcggcgttgt ctggtccatg atgcaaatcc
     2401 cggcaatgac cgtcgtaaat accgccaatg ttttgaatac gcgcacgcgt gcaggtgcag
     2461 taataactta gtccttgctc atgtaaccag gcgagtgctt cgcgataggc gtggtgacgt
     2521 tgcgattgcc agagaacgtc gccgtcccag tgtaggccgt aatgttccag ctggcgcagg
     2581 atagtctctg cggcaccggg aacttcacga ggcgggtcga tatcttcaat gcgtaccagc
     2641 cagcgacctt gccgggcgcg agcctgcaaa tagctgccaa gcgcagcaat cagagaacca
     2701 aaatgaagct cgccggaagg agagggggcg aagcgaccaa tatagtgtgt gtctgtcatc
     2761 tctttgaaca aaaaataagg cgggagcatt tcccgcctgt ggtaaacatg atggaacggc
     2821 tgtaattagc cagccatctg tttttcgcga atttcagcca gcgtcttgca gtcgatgcat
     2881 agatcggctg tcgggcgcgc ttccagacgg cgaataccaa tttcaacacc gcaggattcg
     2941 cagtagccga aatcttcgtc ttccactttt ttcagcgtct tctcgatctt tttgatcagc
     3001 ttacgctcgc gatcgcggtt acgcagttcg aggctgaact cttcttcctg ggctgcacgg
     3061 tctaccgggt ccgggaagtt ggctgcttca tcctgcatat gtgtaacggt gcgatcgact
     3121 tcatccctga gttgattacg ccatgcttcc agaatacgac ggaagtgcgc cagctgggct
     3181 tcattcatat actcttcgcc cggcttctcc tgatatggtt ccaccccagc gatggcgaga
     3241 atactcaggg acgatgtttt acggttttgc ccttcttgca tgttgcttct ccttaacacg
     3301 cactatcgat ccccatgttc gggggaaaaa tgaggccgct ataaatagca gatgcttttc
     3361 cggatagcaa ttatctaaac gtaacacttg acaactgtgt gaggaaaagc gtatttgcgc
     3421 acgcgaccag aatgtaaatt aaccagttac ttactctact acaatgtaac cggtagtgat
     3481 tttttaagag ccatgccttc ggcagaaagt tccgctttgt aagccagaat ttctaccccc
     3541 ctctgttgag cttctgacaa tagttgtgcg tatttctcat cgatgtggcg cgcgggtgaa
     3601 aaccgtgtaa tggctgaatg cagcacggcg aaaaagataa ccgcacgctg gccttcagcc
     3661 gctacgctca tcaactcccg aaggtgtttc tgacctcgtt cagtgaccgc atcgggaaaa
     3721 tatccctgtt cgttctccgc taacgtaacc gatttcactt caatatagca gtctggacgc
     3781 gaatccgcct gcaacataaa gtcaatacgg ctgcgttcgg cgccgtattt tacttcgctt
     3841 ttcagcgagc tatagcctga cagctctgaa attgattcat taaggatagc ctctttcgtc
     3901 aacctgttag cccaaagcgt attgacgcaa ataattgcgc cgctttggct ttgagttaat
     3961 tcccaggtgt gtgggtattt ccgtttggtg ttgtctgaag tcgaatacca gacggtatcg
     4021 ccaggcgttg cacaaccggt catcgcaccc gtattcgggc agtgtagcgt taattcgcga
     4081 ccatcgggtg tgatcacatc ggctaaaaaa cgtttgtaac gctgaattag cgtcgcgcgc
     4141 tgtagagggg gagaaaattc cattgcgaca atccttgtta ttgcgttagc gcccagcgtt
     4201 ttagcggcgt gtagcgtgtg cgtccacggg caaacgagga ggcgtaaagg gtgaactccg
     4261 tcaccgtata cgaccagtta aaacctggcg gcgggattgt caccgcctcg ctggcgtcgc
     4321 gcaataaggt aatatgtgga tgaaacggac gattgctttg aaaacaaccg ctgcgggcag
     4381 cctgtgaacg gagcatattc gccagctgga ttaagccgcg tggcggctgt cgcatcccta
     4441 accacaccac acgcgaacgc agccattgac cggcgtcatc aagcgtgagt gtgaaaccag
     4501 gttgacgaat ccgtccggct aaaagagaaa gcgccttctc tttctctgcg ctcacttcgc
     4561 ctaaaaatgc cagagtcaga tgcaaattat cggcggcgac cggacgtccc gcctcaggtg
     4621 ggaagtgtgt ggcgcgccag tggataatct gttcgcggat ttctgcaggt aagtcgatag
     4681 caaagaacag ccgttgcggt tcagacatgt gaggcactcg gttatgaatt accgcgatgc
     4741 tacaatgtgg cgcgaagaat gttaaccctc tggagcgttt tgtgtcgtcg ttgcccgttg
     4801 ctgccgtctt acctgagtta cttgctgccc tcgatggtgc atcgcaggtg ttattaagtg
     4861 cgccgaccgg ggccgggaaa tcaacctggc tgccgctgca actgctggcg catcccggca
     4921 ttaacgggaa aattatcctg ctggagccgc gtcgtctggc ggcgcgtaac gttgcgcaac
     4981 gactggcgga gctgcttaac gaaaagccag gcgataccgt tggctaccgg atgcgtgcgc
     5041 aaaactgcgt cgggccgaat acccgcctgg aagtggttac cgaaggcgtg ctgacgcaga
     5101 tgatccagcg tgacccggaa ctgagcggtg ttggattggt gatccttgat gaatttcatg
     5161 agcgcagctt gcaggcggat ttggcgttgg cgctgttact cgatgtgcaa caaggtctgc
     5221 gtgatgacct taaactgctg attatgtcgg ctacgctgga caacgaccgc ttgcagcaaa
     5281 tgctgccaga agcgcccgtc gtcatctcag aagggcgctc gtttccagtt gaacgccgtt
     5341 atttgccgct gcccgctcat cagcgttttg acgaagccgt cgcggttgcc accgccgaaa
     5401 tgctgcgtca ggaaagtgga tcattactgt tatttttacc tggcgtcgga gaaattcagc
     5461 gtgtgcagga acaactgact tcacgcatcg gcagtgatgt gttgctctgc ccgctgtatg
     5521 gcgcgttgtc gctgaacgat cagcgaaaag cgatcctccc ggcaccgcaa gggatgcgta
     5581 aagtggtgct ggcgaccaat attgctgaaa ccagtttaac catcgaaggt attcgtctgg
     5641 tggtggattg tgcccaggag cgtgtggcgc gttttgatcc gcgtacaggg cttacgcggc
     5701 tggttactca acgtgttagt caggcgtcga tgacgcaacg tgccgggcgt gccgggcgtc
     5761 tggagccggg tatctgcctg catttaatcg ccaaagaaca agcagaacgc gctgccgcgc
     5821 aaagtgaacc ggaaatatta caaagcgatc tttcctgttt gctgatggaa ttactgcaat
     5881 ggggatgcag cgatccggcg cagatgagct ggctggatca accgccaacg gtgaatctac
     5941 tggccgcgaa acgcctgtta cggatgctgg gggcgctgga cggtgaacgg cttagtgcgc
     6001 aagggcaaaa aatggcagcg ctgggtaacg atccgcgttt agcggcaatg ctggttagcg
     6061 cgaagaacga cgacgaagct gctaccgcgg caaaaattgc cgccattctc gaagagccgc
     6121 cacggatggg caatagtgac ctgggcgtgg cgtttttgcg caatcaaccc gcctggcagc
     6181 aacgtagtca gcaactgtta aaacgtttaa acgtacgcgg cggtgaggca gacagttcgc
     6241 ttatcgcgcc gcgacttgcc ggagcgtatg ccgatcgtat tgctcgccgc cgtgggcaag
     6301 agggacgcta tcaactggcg aacggcatgg gggcgatgct cgatgccgac gatgcgctta
     6361 gccgccacga atggttgatc gcgccgttat tattgcaggg cagttcttcg ccggatgcgc
     6421 ggattttact ggcgctgccg gtcgatattg atgagttagt acaacgctgc ccgcagctgg
     6481 tacagcagtc tgacaccgtg gagtgggatg acgcgcaagg tacgctgaaa gcctggcgtc
     6541 ggctgcaaat cggtcagttg acggtaaaag tgcaaccgct ggcgaaaccc tcggaagacg
     6601 agttgcatca ggcgatgctt aacggcattc gtgataaagg tttaagcgtg ctcaactgga
     6661 cggcggaagc ggaacagcta cgcttgcgtt tgttatgtgc cgcaaagtgg ttgccggaat
     6721 atgactggcc agcggttgat gatgaaagtt tgttggcgac gctggaaacg tggctactgc
     6781 cgcatatgac aggcgtacat tcgctacgcg gcctgaaatc actcgatatt tatcaggcac
     6841 tgcgtggatt acttgattgg gtaatgcagc aacgtctgga tagtgaattg cctgcgcatt
     6901 acactgtgcc gacgggaagc cggatcgcca ttcgttacca tgaagataac ccgcccgcgc
     6961 tggcggtgag aatgcaggag atgtttggtg aggcctctaa tccgactatc gcccaggggc
     7021 gcgtaccgtt ggtgctggag ttgctttccc ctgcccaaag gccgctgcaa atcacgcgtg
     7081 atttgagcgc cttctggaaa ggagcgtacc gtgaggtgca aaaagagatg aaagggcgtt
     7141 atcccaaaca tgtctggccg gacgacccgg caaacaccgc accgacgcga cgaacgaaaa
     7201 agtattcgtg aagaccaaag caaatttgag agatatcttc ttctgtcttg caacagaaga
     7261 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7321 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7381 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7441 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7501 tatggctgct gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7561 atctcgatca aaaaattcgc agccgtattg atggcaaggt ctggcaattg cccgcggcag
     7621 tttatggtcg aatggtcaac cttgagccag acatgaccat cagcaagaac gagatggtga
     7681 agctgctgga ggcgacccag tatcgccagg tgtcgaaaat gacccgtcct ggtgagttta
     7741 ccgtacaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagcaaag
     7801 aagggcaggt gcgcgcgcgt ctgacctttg atggcgatca tctggcgacg atagtcaata
     7861 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgcctgatc accatgatct
     7921 cttcgccaaa cggtgagcag cgtctgtttg tgccgcgcag tggtttcccg gatttgctgg
     7981 tggatacttt gctggcgaca gaagaccgtc atttctacga gcatgatggg atcagtctct
     8041 actcaatcgg acgtgcggtg ctggcaaacc tgactgccgg acgcacggtg cagggggcga
     8101 gtacgctgac gcaacagttg gttaaaaacc tgtttctctc cagcgagcgt tcttactggc
     8161 gtaaagcgaa cgaagcctac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8221 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8281 gcttcccgct ggcgagcttg tattactttg gtcgcccggt agaagaattg agcctcgatc
     8341 agcaggcgct gttggtgggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8401 cgaaactggc gctggagcga cgtaacctgg tactacgttt gctgcaacag caacaaatta
     8461 ttgatcaaga actctatgac atgttgagtg cacgtccgct gggtgttcag ccgcgcggtg
     8521 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagctg caggcgaaat
     8581 taggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8641 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     8701 agttgagcga tcttgaaacc gcgattgtgg ttgttgaccg ctttagcggt gaagttcgtg
     8761 cgatggtcgg tggctctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     8821 gttcgattgg ttcccttgca aaaccagcga cttatctaac ggccttaagc cagccgaaaa
     8881 tctatcgttt gaatacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     8941 aggtctggtc accgcagaac gatgatcgtc gttatagcga aagtggcagg gtgatgctgg
     9001 tagatgcgtt gacccgttcg atgaacgtgc caacggtgaa tctgggcatg gcgttggggc
     9061 tgcctgcggt tacggatacc tggatcaaac tgggtgtgcc gaaagatcaa ctgaatccag
     9121 ttccagcaat gttgttgggg gcgttgaact taacgccaat cgaagtggcg caggccttcc
     9181 agactatcgc cagcggtggt aatcgtgctc cgctttccgc gctacgttca gtcatcgcag
     9241 aagacggcaa ggtgttgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9301 cggcgtatct gacactatgg accatgcagc aggttgtaca acgcggtacg ggacgtcagc
     9361 ttggggcaaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9421 tagatacctg gtttgcgggc attgatggga gcacggtgac catcacctgg gtcggccgtg
     9481 ataacaacca gccgaccaaa ctgtatggtg ccagcggggc aatgtcgatt tatcagcgtt
     9541 atctggctaa ccagacgcca acaccgctga atcttgttcc gccggaagat attgcagata
     9601 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg tatgcgtgtc ttgccagtct
     9661 ggaccagcga tccgcaatcg ctgtgccagc agagtgagat gcagcagcaa ccgtcaggca
     9721 atccgtttga tcagtcttct cagccgcagc aacagcaacc tgctcaacag gagcaaaaag
     9781 acagcgacgg cgtggctggc tggatcaagg agatgtttgg tagtaattaa tatcgtaatc
     9841 gctctccgc
//
LOCUS       DACADF010000158         9741 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.160, whole genome shotgun sequence.
ACCESSION   DACADF010000158 DACADF010000000
VERSION     DACADF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.160"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            182..805
                     /gene="rcsA"
                     /locus_tag="ISB30_003983"
     CDS             182..805
                     /gene="rcsA"
                     /locus_tag="ISB30_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAP1991565.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKDTTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(849..1091)
                     /gene="dsrB"
                     /locus_tag="ISB30_003984"
     CDS             complement(849..1091)
                     /gene="dsrB"
                     /locus_tag="ISB30_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAP1991566.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            1200..1427
                     /gene="yodD"
                     /locus_tag="ISB30_003985"
     CDS             1200..1427
                     /gene="yodD"
                     /locus_tag="ISB30_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAP1991567.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            1723..2538
                     /locus_tag="ISB30_003986"
     CDS             1723..2538
                     /locus_tag="ISB30_003986"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HAP1991568.1"
                     /translation="MFSIQQPLLVFSDLDSTLLDSHSYDWQPAAPWLSRLHEANIPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEICQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FIARLNELGLQFMQGARFWHVLDASAGKDQAANWITATYQQLSGRRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(2535..4229)
                     /gene="yedQ"
                     /locus_tag="ISB30_003987"
     CDS             complement(2535..4229)
                     /gene="yedQ"
                     /locus_tag="ISB30_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAP1991569.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAVYVSRAGFYVSTLPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLKNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(4400..4582)
                     /locus_tag="ISB30_003988"
     CDS             complement(4400..4582)
                     /locus_tag="ISB30_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAP1991570.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(4661..5578)
                     /locus_tag="ISB30_003989"
     CDS             complement(4661..5578)
                     /locus_tag="ISB30_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAP1991571.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5751..6671
                     /gene="yedA"
                     /locus_tag="ISB30_003990"
     CDS             5751..6671
                     /gene="yedA"
                     /locus_tag="ISB30_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAP1991572.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(6660..7130)
                     /locus_tag="ISB30_003991"
     CDS             complement(6660..7130)
                     /locus_tag="ISB30_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAP1991573.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(7111..8529)
                     /locus_tag="ISB30_003992"
     CDS             complement(7111..8529)
                     /locus_tag="ISB30_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP1991574.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAADNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(8596..9291)
                     /locus_tag="ISB30_003993"
     CDS             complement(8596..9291)
                     /locus_tag="ISB30_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP1991575.1"
                     /translation="MELQHWQAQFDNWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVTLAKNHPQRQRSSILAAEETRRLLREEFAQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            complement(9331..9696)
                     /locus_tag="ISB30_003994"
     CDS             complement(9331..9696)
                     /locus_tag="ISB30_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP1991576.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGGIAWYTRPRPARCRRYE"
BASE COUNT         2393 a         2585 c         2355 g         2408 t
ORIGIN      
        1 atcgcaacat ttactttact ttaagacaat tccaggcaaa ttatacaaca ctttacggga
       61 tagtaagtcc gcctgaaaaa ttgcgagagt ggcgcattag gtgacccatg ttgttccgtt
      121 tagtcatgat gaaatattca ggtaagggga attatcgtta cgcattgagt gagggtatgc
      181 catgtcaacg attattatgg atttatgtag ttacacccga ctaggtttaa ccgggtatct
      241 gttgagtaga ggggttaaaa aaagagaaat caacgacatt gaaaccgttg atgaccttgc
      301 catagcttgt gattcacagc gcccttcagt ggtgtttatt aatgaggact gtttcatcca
      361 cgatgcttct aacagtcagc gcatcaagca catcattaat caacatccca atacgttatt
      421 tatcgttttt atggcaattg ccaatgttca ttttgatgaa tatctattgg tcagaaaaaa
      481 tttattgatc agctctaaat cgattaaacc ggaatctctc gacgatatcc ttggcgatat
      541 tctgaaaaaa gatacaacga taacctcgtt tttaaatatg ccgactttat cattgagccg
      601 aaccgaatcg agtatgttgc gaatgtggat ggcaggtcag ggaaccattc aaatctccga
      661 ccaaatgaat atcaaagcca agaccgtttc atcgcataaa ggtaatatta aacgtaagat
      721 caaaacgcat aataaacagg ttatctacca tgtcgtccga ctgacggata atgtgactaa
      781 tggtattttt gtcaacatgc gctaacacat tctgactggt ggtttcccac cagtcaggct
      841 gaataagatt actctgcttt ctcaacaaag ataccgtcct gatgccctgc ttcattaaag
      901 aaccagatgc cgagcgggta gtcttccagc gaaaccaggt acattgtgcc ttcactaaac
      961 tcctcaactg ccagtaccac gccaggacga cgcggaccgc catccgtttt gactgttacc
     1021 cgatcattca ccttcatcgt tttcctcctg tggctttgtg ccagtgtaga acaatttcgt
     1081 ttttctggca gcgccaggcg cgcgcgagtg ctgattttct cgacggtcta tacttaagag
     1141 atgccaggcg gacttaacga ctggcggcaa caacagagta acggttgcga ggaaagatga
     1201 tgaaaaccgc aaaagagtac agcgataccg caaaacgtga ggtcagcgtc gatgtcgatg
     1261 ccctgctggc ggcgatcaat gaaattagcg aaagcgaagt tcatcgcagc cagaacgatt
     1321 ctgaacacgt tagcgtcgat ggacgtgaat atcatacatg gcgtgaattg gcggatgcct
     1381 tcgaactgga tattcatgac ttcagcgtct ctgaagtgaa tcgttgaatg cacaataaaa
     1441 aaatcccgac cctgaggggg tcgggatgaa acttgcttaa gcaagaagca cttaaaaaat
     1501 tcgttacacc aggaaatctg atgtgttcat caccttatct gcaatttttt tcgctgacaa
     1561 gaaaatattc gcgctatgaa tgattaacgc tacttatgaa tagaaatgtg acgcgcgtca
     1621 ccccttggtg gctctggtgt caacgggatc gctcccgaaa aaatcatctt tgattatttg
     1681 tgctgttttg agaatcatcc tggtaagggg agtactgcgc ccatgttttc aattcaacaa
     1741 ccactactgg tttttagcga tcttgatagc accctgctgg acagtcatag ttatgactgg
     1801 caaccggcag ccccctggct cagccgttta cacgaagcca atattcccgt cattctctgt
     1861 agcagtaaaa cttcagcgga aatgctgtac ttgcaaaaaa tgttggggct acaaggctta
     1921 ccgctgattg cagagaatgg cgcagtgatc cagcttgctg aacaatggca ggatatagac
     1981 ggttttccac gcatcatctc aggtattagc catggcgaaa tctgccaggt tttaaatacg
     2041 ctacgcgaga aagaacattt taaattcacg acttttgatg atgtcgacga tgcaaccatt
     2101 gccgaatgga cgggattaag ccgtagccag gcggcgctga cgcagctgca tgaggcgtcg
     2161 gtaacgctaa tctggcgcga cagtgacgag cgtatggcac aatttatcgc tcgtctgaac
     2221 gaactgggct tgcagtttat gcagggtgcg cgcttctggc acgtactgga tgcctctgcc
     2281 ggaaaagatc aggctgccaa ctggattacc gcgacctatc aacaattgtc aggcagacgc
     2341 ccaaccacac ttggcctggg cgatgggcca aacgatgcgc ctttactgga ggtaatggat
     2401 tacgcggtga ttgtgaaagg gctaaaccgt gaaggggtcc atctgcatga tgaggatccg
     2461 gcccgcgtct ggcgaacgca gcgtgaagga ccggaaggct ggcgtgaagg gctggaccat
     2521 tttttctccg cccgttaagc gttatcgctc gcgaataccc gattacgccc ggcctgtttt
     2581 gcgaggtaaa gccgacggtc ggccaatgac tggagttgtt caaaatcata atcaccggtt
     2641 tcctcgctgc tacttacccc cagcgaggca ctgatgcgta tcgttgtact cttggcgatc
     2701 agcatctctt tttcatttaa cttcaggcga atacgttctg cgacctccgc agcctgcgtc
     2761 agattcgcgc ctggcagaat cacacaaaac tcctcaccac cgacccgccc ggcaacgtcc
     2821 tgcgcacgca aggaactgct aattaatccg gcagcatgag aaagaacacg gtcgcccgcc
     2881 tgatgaccaa agcggtcatt aatcgcttta aaatggtcaa gatcgacctg aatgacagaa
     2941 aaaggatgtt ggtgcgtctg acacaattta gcgagcggac gggctttttc gaacaatgcg
     3001 ccacggttat ataaacgcgt taaggtgtcg tgccacgcct gccactgcaa cgagctttgc
     3061 agaacataca tgttgctgac catccggcga atcacatacc aggagatgag taacatggtg
     3121 gtaaagagcg cccacagcag ggttaatgca atgctgatac tgccgaaatc gccgcgcacg
     3181 ccttcgctta gcgtatggac gcgcaccagc acaccgtcga aatggtccag acgttcccag
     3241 ctaacatagc gactgtccat acgaatgccg ccccgcgtgt catgttccat tgcctgcgcc
     3301 agcaaggcca gttcacgagg atcaaaaata ttccctgttg gatggtcagg atttgaagag
     3361 gtcaaaaatc tcagcttact gtcatagagc tgatactcac catcgaggtt tttatcgatg
     3421 gcgtttttta aaaattgctg catagtacgc acgggaatac tcatccccag cacgccatac
     3481 cagtagttat tactgtctac cggaacactg acggtaacct gcggttcagt attgctggcg
     3541 tgttccggtt gcgaagtgaa ccagcgtacc gcgcggtgac gattttctcg ttgtgaatgg
     3601 ccgataaacc agggttgggt gacatagccg taataacgcg ttggtacatt gcgcgtaaac
     3661 aaggtcggca gcgtcgaaac gtaaaatcca gcacgcgaga catacaccgc ctgttcaacc
     3721 atcgacgagg agttgtgcgc cagtcgcagc aagtaaccaa cttccagtgc agcggtaatt
     3781 tcattgtcga ggctttcatt ttcgcgagac aggagattcc cctcgctgac taatgcatcc
     3841 gatacgccat tgactggcag ggtgcgccgt cggttgagtt cgatttgcca ggcgtgctcg
     3901 tcgcgatgct gttcgaactc ggttacggca ttacgcagag aggtgaaatc cagtggcgcc
     3961 acgagagctt cgcgcatgcc attacgcaaa aagatcagtt tgtcgacgtt atactgcaaa
     4021 tgcttatcga gcgcgttggc tacgttttcc agatgattac gctggctgga gatataggca
     4081 tcttccagca ccaccacttc acgccaggtg agcaaggtgg aaaaaagcaa taccacgata
     4141 aagcagagat taacgacatg accgggcccc aggcggcgtg cgagtttttt caaccagctc
     4201 tggttttcca tttttgtctc gtgctgcacc ctgacgactc ccaacctgct tttttatgat
     4261 tctggcataa cccggcgata gcgtaccgca aaacagcgat aattttataa tggtggcatg
     4321 ataagacgcg actatcattc attgcaggca ttgggacacc aggccggatg tgatccggcc
     4381 tggtgttaat gatacccttt caaacttctt ccgcagaacg actcccctcc cccggaacaa
     4441 gctcggtttc atgaaaagct tcccgcttga ccccgtaccc gtcataccaa cgacactcaa
     4501 ccataccgct ggagtatcca gtgacaatca tccgcgggcc gccctcttta accgtaactt
     4561 cctcactaac cataaagctc atactcgcct ccttttttcg agtgaaactt gttcacctta
     4621 gttgaagatg gcgaattttg cataatagcc tgtgcgagta ttaatgtgcc tgaccgcgca
     4681 ttttcgctac ggctttcact cccagcacca cgatgccgcc gatgataaat ccaagaatca
     4741 gatttaaaac agtcggtaat atcatcgcca ccactgcact ttgctgcccg gcgaaatgtt
     4801 caatggcgtg atgcaacggc gcaataccat gtaccacaat cccgccgccg acgaggaaca
     4861 tcgccagcgt gccgacaatc gataacgctt tcatcaacca gggcgcaatg atcaataacc
     4921 ctttacctaa tgcctgcatc agcgcgctgg atttttccgc cagccaatac cccaggtcat
     4981 caatcttaac gataacccct accagaccgt agacgcccac ggtcactacc agcgcgatgc
     5041 ctgaaagcac cagcacctga ttaagcaacg gcgcttctgc cacaatcccc agcgtgatgg
     5101 cgacgatttc cgcagacaat ataaaatcgg tacgaatcgc cccttttatt ttgtcctttt
     5161 caaacttcag cggatcctgc gccgccagct tctccagacg ctgctgactc tgcgccggat
     5221 cttctttatg tttacgcgcc tccagcatat gcagcacttt ctctacccct tcaaagcaga
     5281 gaaacgcgcc gccaatcatc aacagtggcg taatcgccca cgggataaac gcactgatga
     5341 tcagcgccag cggcaccaga atcactttat tgatcagcga acctttcgcc acgccccaga
     5401 ccacgggaag ttcccggttg gcccgcacgc ctgaaacttg ttgcgcattg agcgataagt
     5461 catcccctaa tacaccggcg gttttcttcg ccgccagttt gcccatcacg gagatatcgt
     5521 ccagcagtgt ggcgatatca tcgagcaacg tcagtaaact actcccggcc aaaagcattc
     5581 cctctcattt ttattgttga agggtgtaag tatgaagcaa aaacggcagc tctgatactt
     5641 gcttcacagg tcaacattta tttaacaatc aagtgcaatt atatctctcg ccagcagaat
     5701 gattttcacg tttactatga accacctttt tatttcagcc gtgagggatt atgcgtttcc
     5761 gccagttgtt accgcttttt ggcgcgctgt ttgcgttgta tatcatttgg ggctcaacct
     5821 attttgtcat tcggattggc gtggaaagct ggcctccgtt aatgatggcg ggcgttcgat
     5881 tcctggcagc cggtatttta ttgctggcat ttttgctact gcgcggacac aaacttcccc
     5941 cgctacgtcc gctgctcaat gccgcgctga ttggcctgtt attgctggct gtcggtaatg
     6001 gcatggtgac ggttgccgaa catcaaaatg ttccttccgg catcgccgcc gtagtggttg
     6061 caaccgtgcc cctttttacc ctgtgcttca gccgcctgtt tggcattaaa acgcgcaaac
     6121 tggaatgggt gggtatcgcc attgggcttg ccggaatcat catgctcaat agcggtggaa
     6181 acttaagcgg caatccatgg ggcgcgattc tgattttaat cggctcgatt agctgggcgt
     6241 ttggctcggt ttatggctcg cgcattacct tacctgtagg gatgatggcg ggtgcgattg
     6301 agatgctggc ggctggcgtg gtgttaatga ttgcgtcgat gattgcgggt gaaaaactga
     6361 cggcgctccc ttccctttca ggcttccttg cggtcggcta tctggcgctg tttggttcga
     6421 ttatcgccat taacgcttat atgtatttaa tccgcaacgt cagtccggct ctcgccacca
     6481 gctacgccta cgttaacccg gtggtcgcgg tcttgctggg tacgggactg ggtggcgaaa
     6541 cgctgtcgaa gattgaatgg ctggcgctcg gcgtaattgt cttcgcggtg gtactggtca
     6601 cgttgggaaa atatctcttc ccggccaaac ccgttgtcgc gccagttatt caggatgcat
     6661 caagcgagta aatgaatccc ctgcgtgtcg atctgcgcgc tggcaccttc gccgcagatc
     6721 cactcttcca gacgctcggt aagcgccgca tccgtaagct tctcgcgccc acgtaacgca
     6781 cactcccaga caatcagtac gcgccagccg agttcctgca agcgactgat atcgcggcga
     6841 tcgcgttcaa catttttacc tatcttctcc agccagaact cggttcgagt cgcaggcact
     6901 ttaaacagat agcagtgatg atgatgccag aagcagccgt gggtaaatat cacgcagcga
     6961 tattcatcaa cgacaaaatc cgggcgtccg ggcagactgg cgtcctgaac gcgaaacgca
     7021 aggccctgcc cggttaacaa actggcgagg cgcttctcaa tcgccgtatc acgcgtggca
     7081 atcgcgcgca tatttttgct gcgggtggcc ttatcgtgaa cgtcgaccat gttgtgcctc
     7141 ttgctgacgc aacgccaccg cctgtttgat ttttggctca agcagttttg ccaccgcggc
     7201 aaagaccggc acgaccaccg agttaccgaa ctggcgatag gcctgagtgt ccgaaaccgg
     7261 aatgcggaat ttcgcttctc ccggcgcttc aaaacccatt aagcgcgcgc attcacgagg
     7321 tgttaaccga cgtggacgat gttgctgatt cagcggatcg tcaaagtctt tctcacccgt
     7381 ggccatatcc cagccgcggt cgattaaaat ttccgcgcca tccttgtaat aacgagcaga
     7441 cagcgtacgg gtgacgcttt gcggattgtt cggataaacc attccataac cgaagccgtt
     7501 accgcgcgcc tgatgttttt tcgcatatcg atagaggtac ttccacagca ccggcgtcag
     7561 gatatatttc gcctcaacca tcgggtccag cagctgcgcc agcgtcactc gctgtgcagg
     7621 gaaacattcg ctgatatcac gcagagtaaa atcggctttc agattcagat cgcgacgaaa
     7681 acccaccagc acgatgcgtt cacggtgctg cggcaggaaa tgtttaccgt cgatgatttt
     7741 cggatcgtcc ggcccgttat ctgctgcgtc ggcaacgtca tagcccagtt cgtccagcgt
     7801 ctgcatgatg atgcggaacg tttttccctt atcgtgactc ttcaggtttt tgacattttc
     7861 gagcacaaac atcgccggac gacgcgcgtc gataatgcgc accacatcaa agaacagcgt
     7921 accctgggta tcgcaggcaa aaccgtgcgc ccgcccgagc gagttctttt tcgatacgcc
     7981 agccagcgaa aatggctgac aagggaaacc ggccagtaaa acatcgtgtt caggaatgtg
     8041 ttgacgaata tgttccgccg ccgcctcatc actcacgcct tcttgatgac tgagggtgat
     8101 gtcgcggata tcttcattaa aatgatgcgt cgccgggtcg caataatggt tggctttata
     8161 agtgcgtaca gcatgtttgt tccattcgct ggtaaacacg cactgtccgc caatcgattc
     8221 aaaaccgcga cggatgccgc caattccggc aaatagatcg ataaagcgaa acgcataatg
     8281 cggatgatgt tccggtggtt tgggtaacaa cgtttgcaga tgggcaaact ctttctcact
     8341 taaacggtgc catgccgagt cattcgccag cgcacgtttt aaaattgccg cgctccagtg
     8401 attctcaccc acaccattaa gctgcgccac cagcgtttta acatcataaa tttgcagcag
     8461 tttctccagc attgcctgtg cggcattccc ggtgctgtat gaatcggtta ctgatatatt
     8521 ttcctgcata gatttaaccg gccatctgag atggccgaca ggttaacata attactgccg
     8581 tttagggcca cgcgctcagc cagcgggtga aaacgcatct atcaccttgt gatcgatacc
     8641 ttcattctcc cccgccagtt cggcaccgag cttcgccata aactcgacta aaaattgcgc
     8701 gttgtgctgt gccagctgct tgcctcttgc ggtttgcatg gtttgcggta atttcagtaa
     8761 ctttgtctgg aaatgatcca gcgcgtagcg tttatcatca agcgggcgat gctgtgcgaa
     8821 cggatcttcg ccatcaaaca gcgccaagcc caatgcccct gaaacggcaa atacgcgcgc
     8881 caggccaatc gcccccaggg cttccagccg atccgcatcc tggacgattt tagcttccgt
     8941 tgttaagggg gcgatttgcg cgctgaaact gtgagcggca atggcatgac aaacggcctc
     9001 aattttctcc gccggaaatt gcgcgaactc ttcacggagc aggcgacgcg tctcttctgc
     9061 cgccaggatt gaagaacgct gccgttgcgg atgatttttc gccagggtca cgatatcgtg
     9121 aaaataacag gcggttaaaa tcaccagcat atcgacatcg tcatctgccg ccagtttctg
     9181 tgccgtagcc cagacacggc gaaaatgaca caagtcatgc gccgcgtcct gatgttgatg
     9241 atggtttttc aaccagttat cgaactgtgc ctgccagtgt tgtaactcca tacgcgctcc
     9301 gttgccaaag agtcgcaacc ttagcagttt ttattcatag cgtctgcaac gtgcgggacg
     9361 aggtcgggtg taccatgcaa tgccgcctaa caaggcacca accagggcca taaacagaaa
     9421 tccgaccaat gctcctaacc acttccccgc ggtagatcct aaatcacttt caaatcccga
     9481 cacgggggca tcggggatcc ggctcatcac ccagatgtat cgcgccattg cccagacgaa
     9541 atagccacac catgtataga aagcccacaa agccaatttg ccgccagggc tgcgagagag
     9601 tctctcttcc atctttgtcg aaccgtattc cattttgaga taacccggaa aattcagact
     9661 tagaactatt atgtgataaa agtcacattt ttccacattg gatgagccag tcaagactca
     9721 atcatttttt gaacaaatag a
//
LOCUS       DACADF010000159         9703 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.161, whole genome shotgun sequence.
ACCESSION   DACADF010000159 DACADF010000000
VERSION     DACADF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.161"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            120..1097
                     /gene="csiD"
                     /locus_tag="ISB30_003995"
     CDS             120..1097
                     /gene="csiD"
                     /locus_tag="ISB30_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HAP1991577.1"
                     /translation="MNALTAVQNNAVDSGQDYRGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFQVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNIQGGNSLLLHLDDWEHLDLFFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGTLSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1117..2382
                     /gene="lhgO"
                     /locus_tag="ISB30_003996"
     CDS             1117..2382
                     /gene="lhgO"
                     /locus_tag="ISB30_003996"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HAP1991578.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDIYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKTQFCLAGNRATKVFCDQNGIRYDNCGKMLVATSELEMERM
                     NALWKRTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPPGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFN"
     gene            2408..3856
                     /gene="gabD"
                     /locus_tag="ISB30_003997"
     CDS             2408..3856
                     /gene="gabD"
                     /locus_tag="ISB30_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP1991579.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAASRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDSVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIIGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3870..5150
                     /gene="gabT"
                     /locus_tag="ISB30_003998"
     CDS             3870..5150
                     /gene="gabT"
                     /locus_tag="ISB30_003998"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HAP1991580.1"
                     /translation="MISNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKL"
     gene            5387..6787
                     /gene="gabP"
                     /locus_tag="ISB30_003999"
     CDS             5387..6787
                     /gene="gabP"
                     /locus_tag="ISB30_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAP1991581.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTSAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            6808..7470
                     /gene="csiR"
                     /locus_tag="ISB30_004000"
     CDS             6808..7470
                     /gene="csiR"
                     /locus_tag="ISB30_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAP1991582.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7471..7920)
                     /gene="kbp"
                     /locus_tag="ISB30_004001"
     CDS             complement(7471..7920)
                     /gene="kbp"
                     /locus_tag="ISB30_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAP1991583.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8004..8162)
                     /locus_tag="ISB30_004002"
     CDS             complement(8004..8162)
                     /locus_tag="ISB30_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAP1991584.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8345..8644
                     /locus_tag="ISB30_004003"
     CDS             8345..8644
                     /locus_tag="ISB30_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1991585.1"
                     /translation="MTELAQLQASAEQAAALLKSMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRGEGLIDCQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8654..9178
                     /gene="ygaP"
                     /locus_tag="ISB30_004004"
     CDS             8654..9178
                     /gene="ygaP"
                     /locus_tag="ISB30_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAP1991586.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSVFFLLSGFVGAGLLLAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9225..9629)
                     /gene="stpA"
                     /locus_tag="ISB30_004005"
     CDS             complement(9225..9629)
                     /gene="stpA"
                     /locus_tag="ISB30_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAP1991587.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2227 a         2508 c         2656 g         2312 t
ORIGIN      
        1 aataagaaaa caatatgtaa cttttatgcg catttttcag aaatgtagat atttttagat
       61 tatggctacg aaatgagcat cgccatgtca ccctacatct cataagagga tcgcttctga
      121 tgaatgcact gaccgccgta caaaataacg ctgtcgattc aggccaggac tatcgcggat
      181 tcactctcat cccgtcggcg caatccccgc gtctgctgga actcaccttc accgaacaga
      241 cgaccaaaca gtttctcgaa caggttgccg aatggcccgt gcaggcgctg gagtacaaat
      301 cgtttctgcg ttttcaggta ggcaaaattc tcgacgatct gtgtgcgaat cagctgcaac
      361 cgctgctgtt gaagaccctg ttaaaccgcg ctgaaggtgc gctgttgatc aatgcggtgg
      421 gtatcgatga tgtcgcgcag gcggatgaga tggtgaagct ggcgacggcg gtggcgcatc
      481 tgattggccg ttccaatttc gatgccatga gcggtcagta ttacgcgcgt ttcgtggtga
      541 aaaatgttga taactcagac agctatcttc gtcagccgca ccgcgtaatg gagctgcaca
      601 acgacggcac ttacgtcgaa gagatcactg attacgtgct gatgatgaaa atcgacgagc
      661 aaaacataca gggcggtaac tcgctgctgc tgcatctcga tgactgggaa catctggacc
      721 tctttttccg ccatccgctg gcgcgccgtc cgatgcgctt tgccgcgccg ccgagcaaaa
      781 acgtcagcaa agatgtcttc catccggtgt tcgatgtcga tcagcagggc cgcccagtga
      841 tgcgctatat cgaccagttt gtccagccga aagacttcga agaaggcgtg tggttgagcg
      901 agctttcgga cgccattgaa accagtaaag gcactctttc tgtacccgtt cccgttggca
      961 aattcctgtt gattaacaac ctgttctggc tgcacggtcg cgaccgcttt actccgcacc
     1021 cggatctgcg ccgtgaactg atgcgtcagc gtggctattt cgcttacgcc actcaccact
     1081 accagacgca tcagtaagcg caaaggaatt gagcggatgt atgattttgt gattattggc
     1141 ggcggcatca tcggcatgtc gaccgccatg caactgattg atatctatcc tgacgcccgc
     1201 attgcgttgc tggaaaaaga gtccggccct gcctgtcacc agacgggcca caacagcggc
     1261 gtgatccatg ccggagtcta ttacacgccc ggtagcctga agacgcagtt ttgcctggcg
     1321 gggaaccgcg ccactaaagt cttttgcgat caaaacggca ttcgctacga caactgcggc
     1381 aagatgctgg tcgccacgtc tgaactcgaa atggaacgga tgaatgcgtt atggaaacgc
     1441 acggcggcaa acgggatcga gcgcgagtgg ttaaacgccg aggagctgcg cgagcgtgaa
     1501 ccgaatatca ccgggcttgg cggcattttt gtgccgtcca gcggcattgt cagctaccgc
     1561 gaagtgacgg cggcgatggc aaaaatcttc caggccagag gtggtgagat tatttataac
     1621 gccgaagtca gcgccctcag tgagcataaa aacggcgtgg tgatacgtac ccgtcagggc
     1681 ggtgaatatg aagcatcgac gctgattagt tgttccgggc tgatggctga ccggctggtg
     1741 aaaatgctcg gcctcgaacc gggctttatt atctgcccgt tccgcggcga gtatttccgc
     1801 cttgcgccag agcataacca gattgttaac cacctgattt accccattcc cgacccggca
     1861 atgccgtttt tgggcgtgca tctcacccgc atgatcgacg gcagcgtgac cgtcgggcca
     1921 aacgcggtgc tggctttcaa acgcgaaggc taccgcaagc gcgacttctc atttagtgac
     1981 acgctggaga ttttaggctc gtcggggatt cgccgggtgc tgcaaaacca tctacgctca
     2041 ggactgggcg agatgaaaaa ctcgctgtgc aaaagcggct atctgcggct ggtgcaaaag
     2101 tattgtcccc ggctttcgtt aagcgatctc cagccctggc cccccggtgt gcgggcgcag
     2161 gcggtatcgc cggacggcaa gctgattgac gattttctgt ttgtcaccac cccgcgcacg
     2221 atccacacct gtaatgcgcc ctccccggca gcgacatcag caattcctat tggtgcgcat
     2281 attgtcagca aggtacaaac gctgttggca agccagagta accccggacg cacgctgcga
     2341 gcggcacgta gtgtggatgc cttacacgcc gcatttaatt aataaccttt gaaaacagga
     2401 tgtagcgatg aaacttaacg acagtaactt attccgccag caggcgttga ttaacgggga
     2461 atggctggac gccaacaatg gcgaggtcat cgacgtcacc aatccggcga acggcgacaa
     2521 gctgggtagc gtgccgaaaa tgggcgctga tgaaacccgc gccgctatcg acgccgccag
     2581 ccgcgccctg cccgcctggc gtgcgctcac tgccaaagaa cgcgccaaca ttctgcgcaa
     2641 ctggttcaat ttaatgatgg agcatcagga cgatttagcg cgcctgatga ccctcgaaca
     2701 gggcaaacca ctggctgaag cgaaaggcga aatcagctac gccgcctcat ttattgagtg
     2761 gtttgctgaa gaaggcaaac gtatttatgg cgacaccatt cccggtcatc aggccgataa
     2821 acgcctgatt gtgatcaagc agccgattgg cgtcaccgcg gctatcacgc catggaactt
     2881 cccggcggcg atgattaccc gtaaagctgg tccggcgctg gcggctggct gcaccatggt
     2941 gctgaagccc gccagtcaga cgccgttctc tgcgctggcg ctggcagaac tggcgattcg
     3001 cgcgggcgtt ccggctggcg tgtttaacgt ggtcaccggt tcggcgggcg cggtcggtaa
     3061 cgaactgacc agcaacccgc tggtgcgcaa gctgtcgttt accggctcga ccgaaattgg
     3121 ccgtcagtta atggaacagt gcgcgaaaga catcaaaaaa gtgtcgctgg agctgggcgg
     3181 caacgcaccg tttattgtct ttgacgatgc cgatctcgat aaagccgtgg aaggcgcgct
     3241 ggcctcgaaa ttccgtaacg ccgggcaaac ctgcgtctgc gccaaccgcc tgtatgtgca
     3301 ggacagcgtg tatgaccgct ttgccgaaaa attgcagcag gcggtgagca aactgcacat
     3361 cggcgacggg ctggagaaag gcgtcaccat cgggccgttg atcgatgaaa aagcggtagc
     3421 aaaagtggaa gagcatattg ctgatgcgct ggagaaaggc gcgcgcgtgg tttgcggcgg
     3481 taaagcacat gaacgcggcg gtaacttctt ccagccgacc attctggtag acgttccggc
     3541 taacgccaaa gtgtcgaaag aagagacgtt tggccccctc gccccgctgt tccgttttaa
     3601 agatgaagcc gatgtgattg cgcaagccaa tgacaccgaa tttggacttg cagcctattt
     3661 ctacgcccgt gatttaagcc gcgtcttccg cgtgggcgaa gcactggagt acggcatcat
     3721 cggcatcaac accggcatta tttccaatga agtggccccg ttcggcggca ttaaagcctc
     3781 gggtctgggt cgtgaaggtt cgaagtatgg catcgaagat tacttagaaa tcaaatatat
     3841 gtgcatcggt ctttaactgg agaatgcgaa tgatcagcaa taaagagtta atgcagcgcc
     3901 gcagtcaggc gattccccgt ggcgttgggc aaattcaccc gattttcgct gaccgcgcgg
     3961 aaaactgccg ggtgtgggac gttgaaggcc gtgagtatct tgatttcgcg ggcgggattg
     4021 cggtgctcaa taccgggcac ctgcatccga aagtggttgc ggcggtggaa gcgcaattga
     4081 aaaaactgtc gcacacctgc ttccaggtgc tggcctacga gccgtatctg gagctgtgcg
     4141 agattatgaa tcagaaggtg ccgggcgatt ttgccaagaa aactctgctg gttacgaccg
     4201 gttccgaagc ggtggaaaat gcggtgaaaa tcgcccgcgc cgccactaaa cgtagcggta
     4261 ctatcgcctt tagcggtgcg tatcacgggc gcacgcatta cacgctggcg ctgaccggca
     4321 aggtgaatcc gtactctgcg ggcatggggc tgatgccggg tcatgtttat cgcgcgcttt
     4381 atccttgccc gctgcacggc ataagcgagg atgacgctat cgccagcatc caccggatct
     4441 tcaaaaatga cgccgcgccg gaagatatcg ccgccatcgt gattgagccc gttcagggcg
     4501 aaggcggttt ctacgccgcg acgccagcct ttatgcagcg tttacgcgcg ctgtgtgacg
     4561 agcacgggat catgctgatt gccgatgaag tgcagagcgg cgcagggcgt accggcacgc
     4621 tgtttgcgat ggagcaaatg ggcgtggcac cagatctcac cacctttgcg aaatcgatcg
     4681 caggcggttt cccgctggcg ggcgtcaccg ggcgcgcgga agtgatggac gccgtcgcac
     4741 cgggcggact gggtggcacc tatgccggta acccgattgc ctgcgtagcg gcgctggaag
     4801 tgttgaaggt gttcgagcag gaaaatctgc tgcaaaaagc caacgttctg gggcagaagc
     4861 tgaaagacgg attgctggcg atagccgaaa aacacccgga gatcggcgac gtacgcggtc
     4921 tgggggcgat gatcgccatc gagctgtttg aagatggcga tcacagcaaa ccggacgcaa
     4981 aactcaccgc cgagatcgtg gcacgcgccc gcgataaggg tctgattctt ctctcctgcg
     5041 gcccgtatta caacgtgctg cgcatccttg taccgctcac cattgaagac gctcagatcc
     5101 gtcagggtct ggagatcatc agccagtgtt ttgctgaggc gaagctgtag caccgcacca
     5161 tgccggaggc gacgctttgc ggcttgtccg gcctacgaga atccaggtcg gataaggcgt
     5221 ttacgccgca tccgacaatc tgtacgtgaa caggaataaa tctatgtcgg ccggataagg
     5281 cggagccgct ctccggcaaa aagaaccaat aacaattaaa cgcgtgaccc gacgcgggaa
     5341 atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg ggcaatcatc
     5401 gcaaccacat gagttaggcg gcgggctgaa gtcacgccac gtcaccatgt tgtctattgc
     5461 cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg aagcgggccc
     5521 ggcggtatta ctggcctatc tgttcgccgg attactggtg gttatgatta tgcggatgtt
     5581 ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg ccgataaagc
     5641 cattggccgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt gggtactggt
     5701 tatcccactt gaagccaaca tcgccgccat gatcctgcac tcatgggttc caggcattcc
     5761 catctggtta ttttccctcg tcattaccct cgccttaacc ggcagtaatt tattaagcgt
     5821 taaaaactac ggcgaatttg agttctggct ggcgctgtgt aaagtcatcg ctatcctggc
     5881 ctttattttc cttggtgccg tcgcaattag cggtttttac ccgtatgccg acgtgagcgg
     5941 gatttcaaga ttgtgggata gcggcggctt tatgcccaac ggcttcggtg cggtattaag
     6001 cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca ttgccgccgc
     6061 ggaatccgac acgccggaaa aacatattgt tcgcgccacc aactcggtta tctggcgtat
     6121 ctctattttc tatttgtgtt ctatttttgt cgttgttgca ttaatcccgt ggaatatgcc
     6181 agggctgaaa gccgttggtt cttatcgctc ggttctggaa ttgctcaata ttcctcatgc
     6241 gaaattaatc atggactgcg tgatattact ttccgtaacc agttgcctga actcggcgct
     6301 gtataccgct tccaggatgc tctactcctt aagccgtcgc ggtgatgctc ccgcggtaat
     6361 gggtaaaatc aaccgcagta aaaccccgta cgtagcggtg ttactctcca ccagcgcggc
     6421 attcttaacg gtggtggtga actattacgc gcctgcgaag gtgtttaaat ttctgatcga
     6481 cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac aattgcggat
     6541 gcgcaaaatt ctgcgagcag aaggaagcga aattcgcctg cggatgtggc tttatccgtg
     6601 gctcacctgg ctggtcatcg gctttattac ctttgtgttg gtagtgatgc tattccgccc
     6661 ggcgcaacag ttagaagtga tctccaccgg cttattagcg attgggatta tctgtaccgt
     6721 gccaattatg gctcgctgga aaaagctgat aatgtggcaa aaaacaccca ttcataatac
     6781 gcgctgataa tgatcaggag tcacaccatg accattacct ctctggatgg ctatcgctgg
     6841 ctgaagaacg atattattcg cggcaatttt cagccggatg aaaaattacg gatgagtttg
     6901 ctgacatcgc gttatgcact tggcgttggg ccgttgcggg aagcactttc gcaactggtg
     6961 gcagaacgac tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag
     7021 caggagctgc tcgatatttt cgacgcccgc gccaatatgg aagcgatgct ggtgagtctg
     7081 gcgattgccc gcggtggcga tgaatgggag gcagacgttc tcgcaaaagc gcatctgctc
     7141 agtaagcttg aggcctgtga cgccagtgag aaaatgcttg atgagtggga tctgcgtcat
     7201 caggcgtttc atactgcaat tgtggcgggc tgtggttctt actatttgct gcaaatgcgt
     7261 gaacggttgt ttgatctggc agcgcgttat cgatttatct ggctgcggcg aacagtgctt
     7321 tcggtggaaa tgctggaaga taaacacgat cagcaccaga ccctgactgc ggcggtactg
     7381 gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac gccaattccc
     7441 attatccagc aggcgatggc tggcaattaa ctactcttca ggaatacgca acacttgccc
     7501 cgggtaaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga ttttattgta
     7561 cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc cagacttaac
     7621 ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat cgacactggc
     7681 aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct gacttaagcc
     7741 gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat cggcatcagg
     7801 tataccggtt ttgttcagat gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg
     7861 acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga acagacccat
     7921 agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg cggcgcgtta
     7981 acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa
     8041 ccggggatat agccaagcag tgtcagcaga atattaataa tgaacgccca accgaaccct
     8101 ttaccgagca gcacgccgag cggcggcaga atgatggtga tgacgattct ccagaaaccc
     8161 atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat ctcttaagcg
     8221 tagtaatttt agcggagcct gccagtttca gcgtacgctt ttactctcat ttacgctgct
     8281 gatgctggta tatgttgcac acttagttta gagtttactt aatttagaaa atacttaaac
     8341 aactatgact gaactcgcac aattacaggc cagtgccgaa caggcagcgg ccttattgaa
     8401 atcaatgagc caccctaaac gattgctgat tctgtgtatg cttagcggtt cccccggcac
     8461 cagcgcggga gagctgacgc gcattaccgg actgagtgcc tctgcgacat cacagcatct
     8521 cgctcgtatg cggggcgaag ggcttatcga ctgccaacgg gatgcccaac gcattctata
     8581 ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg tctattgtcc
     8641 gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag aattaatcgc
     8701 acgcggcgca aagttaatcg atattcgtga tgctgatgaa tatcttcgtg aacatattcc
     8761 tgaagcagat ctggctccat tatccgtact ggaacagtca ggtcttccgc ctaaattacg
     8821 tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata acgctgataa
     8881 attagcagcg attgccgccc ccgcagaaat ctttttactc gaagatggga ttgatggctg
     8941 gaaaaaagca ggattgccag tagcggtaaa taaatcccaa cccttaccgt taatgcgcca
     9001 ggtgcaaatc gccgcgggtg gtttaatatt aatcggcgtt gtactgggtt ataccgtaaa
     9061 tagcgttttc ttcttattaa gcggctttgt tggtgctggg ttactgcttg caggaattag
     9121 tggtttttgt ggaatggcaa ggttgttaga taagatgccg tggaaccaac gagcttgagc
     9181 agcgacgccg gacgcgccat agcagcgaca tccggcctca gtaattagat caggaaatcg
     9241 tcgagagatt taccttctgc cagcgcctga gcgattggct tcggtgtacg gccctgaccg
     9301 gtccaggttt tagtttcacc gttaacatcg atgaatttat atttcgccgg acgcggctgg
     9361 cgttttttac cagcgcgtgg tgccgcagca gagctattac ccaataactc ttccgggtta
     9421 attccgtcag ctttcatcag ctccagccag gtgctaattt tttcctggcg ctctgccagt
     9481 tcacgctgct gttgttcttc ttcttcacgt ctttctttag tgacaaccct gaatttttcg
     9541 agcatttctt caagaacgtc aatggagaat tcgcgagcca tcgcacggag ggtgcgaatg
     9601 ttatttaaac tttgtaacat tacggacata ataaagaaaa ccttttaacg ccaaaacaaa
     9661 aaagtatttc gtcgcgtagt gtaagcgatc acttattata ttt
//
LOCUS       DACADF010000160         9632 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.162, whole genome shotgun sequence.
ACCESSION   DACADF010000160 DACADF010000000
VERSION     DACADF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.162"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..1165
                     /gene="trkD"
                     /locus_tag="ISB30_004006"
     CDS             <1..1165
                     /gene="trkD"
                     /locus_tag="ISB30_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Responsible for the low-affinity transport of
                     potassium into the cell; involved in potassium ion uptake
                     under hyper-osmotic stress at a low pH; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP1991588.1"
                     /translation="GHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNPFFL
                     LAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMESGQI
                     YIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHWNKY
                     FVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRRMHE
                     HGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILLTLR
                     TEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMMETSF
                     FMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            1332..1751
                     /gene="rbsD"
                     /locus_tag="ISB30_004007"
     CDS             1332..1751
                     /gene="rbsD"
                     /locus_tag="ISB30_004007"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP1991589.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQQHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            1759..3264
                     /gene="rbsA"
                     /locus_tag="ISB30_004008"
     CDS             1759..3264
                     /gene="rbsA"
                     /locus_tag="ISB30_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAP1991590.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKLQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            3269..4234
                     /gene="rbsC"
                     /locus_tag="ISB30_004009"
     CDS             3269..4234
                     /gene="rbsC"
                     /locus_tag="ISB30_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAP1991591.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            4259..5149
                     /gene="rbsB"
                     /locus_tag="ISB30_004010"
     CDS             4259..5149
                     /gene="rbsB"
                     /locus_tag="ISB30_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HAP1991592.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            5275..6204
                     /gene="rbsK"
                     /locus_tag="ISB30_004011"
     CDS             5275..6204
                     /gene="rbsK"
                     /locus_tag="ISB30_004011"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP1991593.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKTLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            6208..7200
                     /gene="rbsR"
                     /locus_tag="ISB30_004012"
     CDS             6208..7200
                     /gene="rbsR"
                     /locus_tag="ISB30_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAP1991594.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(7166..8593)
                     /locus_tag="ISB30_004013"
     CDS             complement(7166..8593)
                     /locus_tag="ISB30_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAP1991595.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDTTGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIWLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(8616..9308)
                     /locus_tag="ISB30_004014"
     CDS             complement(8616..9308)
                     /locus_tag="ISB30_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP1991596.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2388 a         2352 c         2542 g         2350 t
ORIGIN      
        1 ggggcacttt ggtaagttcc ctattcgtct ggcgtggttc accgtcgtat tgccttcctt
       61 aacccttaat tacttcggcc agggagcgct gttgttaaag aatccggaag cgattaagaa
      121 cccgttcttc ctgttggcac cggactgggc gctgatcccg ctgctgatca tcgccgcact
      181 ggcgacggta attgcctcgc aggcggttat ctctggcgtc ttctcattga cgcgtcaggc
      241 ggtacgtctg ggatatttgt cgccgatgcg cattattcac acctccgaaa tggagtcagg
      301 gcaaatctat attccctttg tgaactggat gctctatgtc gcggtcgtga ttgtgattgt
      361 cagctttgag cactccagca acctggcggc ggcgtacggg attgcggtga ccggaaccat
      421 ggtgctgacg tctattctct cgactaccgt ggcacgtcag aactggcact ggaataagta
      481 ttttgttgcg ctgatcctga ttgctttcct ttgtgtcgat attcccttgt tcaccgctaa
      541 cctcgataaa ctgctctccg gtggctggtt gccattgagc ctcggtacgg tgatgtttat
      601 cgtgatgacc acctggaaga gcgagcgttt ccgcttgctg cggcggatgc atgaacatgg
      661 taactctctg gaagcgatga ttgcttcgct ggagaaatca ccgcccgttc gcgtgcctgg
      721 gaccgcggtg tatatgtcgc gtgcaatcaa cgtcattccc tttgcgctga tgcataacct
      781 taaacataac aaggtattgc atgagcgggt gattctgtta actctgcgca ccgaagacgc
      841 gccatatgtc cataacgtcc gtcgggtaca gattgaacaa ctgtcgccca ctttctggcg
      901 cgtggtggca agttatggtt ggcgagaaac gccaaacgtg gaagaagttt tccaccgctg
      961 cggtctggaa ggattaagct gccggatgat ggaaacctcc ttctttatgt cgcatgagtc
     1021 gttgatcctc ggcaaacgcc cgtggtattt gcgtctgcgc ggcaagctgt acttgctgct
     1081 gcaacgtaat gcgctgcggg caccggatca atttgaaatc ccgccaaaca gagttatcga
     1141 actgggtact caggtcgaaa tctaacgcca gacgcctcct ttctccataa gggggcgttt
     1201 ttgttttcat ggttaatcac catgtaaaac gtttcgaggt tgatcacatt tccgtaacgt
     1261 cacgatggtt ttcccaactc agtcaggatt aaactgcggg tcagcgaaac gtttcgctga
     1321 tggagaaaaa aatgaaaaaa ggcaccgttc ttaattctga tatttcatcg gtgatctccc
     1381 gactgggaca taccgatacg ctggtggtgt gtgatgctgg tttacccatc cccaaaagta
     1441 caacgcgtat cgatatggca ttaacccagg gtgtaccttc ttttatgcag gtgctgggcg
     1501 tcgtcacaaa tgaaatgcag gtcgaggctg tcattatcgc ggaagagatc aaacaacata
     1561 atccgcaact ccacgaaacg ttgctcactc accttgagca gctgcaacaa caccagggaa
     1621 acaccattga aattcgttac accacgcatg aacaattcaa acaacaaacc gcagaaagtc
     1681 aggcggtaat tcgcagcgga gaatgttctc cgtatgcgaa tatcattctc tgtgctggcg
     1741 tgacgttctg aggccgtcat ggaagcatta cttcagctta aaggcatcga taaagccttt
     1801 ccgggcgtaa aagccctctc gggcgcagcg ttaaatgtct atccgggccg cgtgatggcg
     1861 ctggtgggcg aaaacggcgc gggtaaatcc accatgatga aagtgcttac tggtatctat
     1921 actcgcgatg ccggtacgct tttatggttg gggaaagaaa cgacatttac cgggccgaaa
     1981 tcttcccagg aagccgggat tgggattatc caccaggaac tgaacctgat cccgcagtta
     2041 accattgccg aaaacatttt cctcggtcgt gagtttgtta atcgctttgg caaaattgac
     2101 tggaaaacca tgtatgccga agcggataaa ttgctggcta aacttaacct gcgctttaaa
     2161 agcgacaaac tggtgggtga tctttccatc ggtgaccagc aaatggttga aatcgccaaa
     2221 gtgctgagct ttgagtcgaa agtcatcatt atggatgaac cgaccgatgc gctgaccgat
     2281 accgaaaccg aatccttgtt ccgcgtcatc cgcgagctga aattgcaagg ccgcggtatt
     2341 gtctatatct cccaccgcat gaaagaaatc ttcgagattt gcgatgacgt taccgttttt
     2401 cgtgacggac aatttattgc tgagcgtgaa gtggcatcac tgaccgaaga ttctctgatt
     2461 gagatgatgg tgggccgcaa gctggaagat caatatccgc acctggacaa agcgccggga
     2521 gatatccgcc tgaaagtcga taatctctgc ggacctggcg ttaacgatgt ctcttttact
     2581 ttacgcaaag gcgaaattct tggcgtctct ggtttgatgg gcgctgggcg taccgaactg
     2641 atgaaagtgc tctacggcgc actgccgcgc acaagcggtt acgtcaccct ggatgggcat
     2701 gaagtcgtta cccgttcgcc gcaggatggc ctggcaaacg gcattgttta tatctccgaa
     2761 gaccgtaaac gtgacggttt agtgctgggc atgtcagtaa aagagaacat gtcgctgacc
     2821 gcgctgcgct acttcagccg cgctggcggc agtttgaagc atgccgatga acagcaggct
     2881 gtgagtgatt tcattcgtct gtttaatgtg aaaacgccat cgatggaaca ggcaattggt
     2941 ctgctttccg gtggcaatca gcaaaaagta gcgattgccc gtggtctgat gacacgccct
     3001 aaagtgttga tcctcgatga gcctacccgt ggcgtagatg tcggcgcgaa aaaagagatc
     3061 tatcaactga ttaaccagtt caaagccgat ggcttgagca tcattctggt gtcatcggag
     3121 atgccagaag tattaggcat gagcgatcgc atcatcgtca tgcatgaagg gcatctcagc
     3181 ggggaattta ctcgtgagca ggccacccaa gaagtgttaa tggctgccgc tgtgggcaag
     3241 cttaatcgcg tgaatcagga gtaaaaaaat gacaacccag actgtctctg gtcgccgtta
     3301 tttcacgaaa gcgtggctga tggagcagaa atcgcttatc gctctgctgg tgctgatcgc
     3361 gattgtctcg acgttaagcc cgaacttttt caccatcaat aacttattca atattcttca
     3421 gcaaacctcg gtgaacgcca ttatggcggt cgggatgacg ctggtgatcc tgacgtcggg
     3481 tatcgacttg tcggtaggtt ctctgttggc gctgaccggc gcagttgctg catctatcgt
     3541 cggcattgaa gtcaatgcgc tggtggctgt cgctgccgct ctcgcgttag gtgccgcaat
     3601 tggtgcggta actggggtga ttgtagcgaa aggtcgcgtc caggcgttta tcgctacgct
     3661 ggttatgatg cttttactgc gcggcgtgac catggtttat accaatggta gcccagtgaa
     3721 taccggcttt actgagaacg ccgatctgtt tggctggttt ggtattggtc gtccgctggg
     3781 cgtaccgacg ccagtctgga tcatggggat tgtcttcctc gcggcctggt acatgctgca
     3841 tcacacgcgt ctggggcgtt acatctacgc gctgggcggc aacgaagcgg caacgcgtct
     3901 ttctggtatc aacgtcaata aaatcaaaat catcgtctat tctctttgtg gtctgctggc
     3961 atcgctggcc gggatcattg aagtggcgcg tctctcctcc gcacaaccca cggcggggac
     4021 tggctatgag ctggatgcta ttgctgcggt ggttctgggc ggtacgagtc tggcgggcgg
     4081 aaaaggtcgc attgttggga cgttgatcgg cgcattaatt cttggcttcc ttaataatgg
     4141 attgaatttg ttaggtgttt cctcctatta ccagatgatc gtcaaagcgg tggtgatttt
     4201 gctggcggtg ctggtagaca acaaaaagca gtaataacga ctacaggaca tcttgaatat
     4261 gaacatgaaa aaactggcta ccctggtttc cgctgttgcg ctaagcgcca ccgtcagtgc
     4321 gaatgcgatg gcaaaagaca ccatcgcgct ggtggtctcc acgcttaaca acccgttctt
     4381 tgtatcgctg aaagatggcg cgcagaaaga ggcggataaa cttggctata acctggtggt
     4441 gctggactcc cagaacaacc cggcgaaaga gctggcgaac gtgcaggact taaccgtccg
     4501 cggcaccaaa attctgctga ttaacccgac cgactccgac gcagtaggta atgctgtgaa
     4561 gatggctaac caggcgaaca tcccggttat cactcttgac cgccaggcaa cgaaaggtga
     4621 agtggtgagc cacatcgcat ctgataacgt actgggcggc aaaatcgccg gtgattacat
     4681 cgcgaagaaa gcgggtgaag gtgccaaagt tatcgagctg caaggcattg ccggtacatc
     4741 cgcagcccgt gaacgtggcg aaggtttcca gcaggccgtt gctgctcaca agtttaatgt
     4801 tcttgccagc cagcctgcag attttgatcg caccaaaggc ttgaacgtaa tgcagaacct
     4861 gttgaccgct catccggatg ttcaggctgt attcgcgcag aatgatgaaa tggcgcttgg
     4921 cgcgctgcgc gcactgcaaa ctgccggtaa atcggatgtg atggtcgtcg gatttgacgg
     4981 tacaccggat ggcgaaaaag cggtgaatga tggcaaacta gcagcgacta tcgctcagct
     5041 acccgatcag attggcgcga aaggcgtcga aaccgcagat aaagtgctga aaggagagaa
     5101 agttcaggct aagtatccgg ttgatctgaa actggttgtt aagcagtagt tttaatcagg
     5161 ttgtatgacc tgatggtgac ataaatacgt catcgacaga tgaacgtgta atataaagaa
     5221 aagcagggca cgcgccaccc taacacggtg gcgcatttta tggacatccc gaatatgcaa
     5281 aacgcaggca gcctcgttgt tcttggcagc attaatgctg accacattct taatcttcaa
     5341 tcttttccta ctccaggcga aaccgtaacc ggtaaccact atcaggttgc atttggcggc
     5401 aaaggcgcga atcaggcggt ggctgctggg cgtagcggtg cgaatatcgc gtttattgcc
     5461 tgcactggcg atgacagcat tggtgagagc gttcgccagc agctcgccac tgataatatc
     5521 gatatttctc cggtcagcgt gatcaaaggc gaatcaacag gtgtggcgct gatttttgtt
     5581 aatggcgaag gtgagaatgt catcggtatt catgccggcg ctaatgctgc cctttccccg
     5641 gcgctggtgg aagcgcaacg tgagcgtatt gccaacgcgt cggcattatt aatgcagctg
     5701 gaatcaccac tcgaaagtgt gatggcagcg gcgaaaatcg cccatcaaaa taagactatc
     5761 gttgcgctta acccggctcc ggctcgcgaa cttcctgacg aacttctggc gctggtggac
     5821 attattacgc caaacgaaac ggaagcagaa aagctcaccg gtattcgtgt tgaaaatgat
     5881 gaagatgcag cgaaggcggc gcaggtactg catgaaaaag gtatccgtac tgtactgatt
     5941 actttaggaa gtcgtggtgt atgggctagc gtgaatggtg aaggtcagcg cgttcctgga
     6001 ttccgggtgc aggctgtcga taccattgct gccggagata cctttaacgg cgcgttaatc
     6061 acggcgttgc tggaagaaaa aacattgcca gaggcgattc gttttgccca tgcagccgct
     6121 gcgattgccg taacacgtaa aggcgcacaa ccttccgtac cgtggcgtga agagatcgac
     6181 gcatttttag acaggcagag gtgacgcttg gctacaatga aagatgttgc ccgcctggcg
     6241 ggcgtttcta cctcaacagt ttctcacgtt atcaataaag atcgcttcgt cagtgaagcg
     6301 attacagcca aagttgaagc ggcgattaaa gaactcaatt acgcgccatc agctctggcg
     6361 cgtagcctca aactcaatca aacacatacc attggcatgt tgatcactgc cagtaccaat
     6421 cctttctatt cagaactggt gcgtggcgtt gaacgcagct gcttcgaacg cggttatagt
     6481 cttgtccttt gcaataccga aggcgatgaa cagcggatga atcgtaatct ggaaacgctg
     6541 atgcaaaaac gcgttgatgg cttgctgtta ctgtgcaccg aaacgcatca gccttcgcgt
     6601 gaaatcatgc aacgttatcc gacagtgcct accgtgatga tggactgggc tccgttcgat
     6661 ggcgacagcg atcttattca ggataactcg ttgctgggcg gagacttagc aacgcaatat
     6721 ctgatcgata aaggtcatac ccgtattgcc tgtattaccg gtccgctgga taaaactccg
     6781 gcgcgcctgc ggttggaagg ttatcgggct gcgatgaaac gtgcgggtct caacattcct
     6841 gatggctatg aagtcactgg tgattttgaa tttaacggcg ggtttgacgc tatgcgccaa
     6901 ctgttatcac atccgctgcg tcctcaggcc gtctttaccg gaaatgacgc tatggctgtt
     6961 ggcgtttacc aggcgttata tcaggcagag ttacaggttc cgcaggatat cgcggtgatt
     7021 ggctatgacg atatcgaact ggcaagcttt atgacgccac cattaacaac tatacaccaa
     7081 ccgaaagatg aactggggga gctggcgatt gatgtactca tccatcggat aacccagccg
     7141 accctacagc aacaacgatt acaacttact ccgattctga tggaacgcgg ttcggcttag
     7201 atttacgctg tcttttgatc aaattattac catcggttgt tttcagtagc atgaacattg
     7261 ctgctgaagc aacagtaata atgcccatcg tgataaacgt atagtggaat tgttcgactg
     7321 tcgttgtgcc ttccatccct tcgtaaacgc gaaggacggc cgcacttaca gcaacgccta
     7381 aactgataga cagttgctgc gtgaccgcca gaacactgtt accgctgctg gcgttgtcat
     7441 cggtcagatc ggcaagtgtg atggtattca tcgcggtaaa ttgcgtcgac atagccatcc
     7501 ctaatataaa caacggcaag atcagcatcc atatcgccat tgctggtgat tgcaaagaga
     7561 actgagcgat cattagccca ataatcaccg tgatccccac taacgtatgg cgatagccca
     7621 gacgacgtaa gacttgggta accatcgatt ttgcaataat ggaacctaac gctgtcggtg
     7681 ccatcataca gccagcaata aacgcctgat acccaaatcc tacctgtaac atcaatggca
     7741 taaggaacgg tacacagccg gtccccagac gggttgcaat attgcctacg ataccgatcg
     7801 agaaagtgcg ggttttaaat aaatctaatg aaattaatgg gtttggcgtg cgtcgcgcat
     7861 ggagaatata gagaagcagt aaccagatgc tggtgacaat taccgtcaag gcaatccagc
     7921 tggcgacaat cttttccccg aatagctcta ttccgcttga gaagagaaca aggctgaggc
     7981 caaacagcaa aaagccagtg gtatcgaatc tgcgtcgtgc ggtggtgaaa ttgggcatat
     8041 gtttgcgcgc gtaaagaagg cccgcaatac ctatggggat attgattaaa aatatccagt
     8101 gccaggttgc ccaggtgacc agcacgccgc caagaacggg gcctaaaatt ggccccacca
     8161 gacccggcat ggcgacaaaa ttcaatacag gaagaagttc attacgagga taagcgcgca
     8221 gtaaggccag ccgagcaaca ggcatcatca ttgcgccgcc tatcccctga ataacccgga
     8281 agacaaccag ctgtggtagc gagttggaaa gtgcgcaggc cagagaaccc aacgtgaaca
     8341 gactcacggc aagggtaaaa atgcgacgcg taccgaagcg atcggctagc catccgctta
     8401 ccggaataag catcgccacc gtcagcgtat aactgatgat ggctgattgc atcgcgagag
     8461 gagaacgatt aaggctatga gcgattgcgg gtaaggcggt attcagaata gtggcatcaa
     8521 gtgcctgcat gaagaaggcc atcgccgcga tccacggcaa acccgccata ctgcgcttct
     8581 ttttatcgct cattcaaagt cctgttatcg ggttatcact tatctggtga gcgtagcagc
     8641 gcctgacaag ctttaaatgc cgcgtcgcca tcgctttgga taatcgcatc gacaatcgcc
     8701 tggtgcagat ccagctttat cactgtgtcg ctggtaattg acgtgaagta agtgtgataa
     8761 accgaatgga atagcgaggc gaatgaggtc aaaaacggat tggcgctcat ttcatagata
     8821 tgctcatgcc aggccatatc gacttcgatc cagcgttcac ggcgaaagtt ctcttttaat
     8881 gccgccattt cggccattaa cgtattgaga tgcgccttct gttccgcggt gccaaccgtt
     8941 gctgccagta ggcaagcttg cggctccaga cagatacgca taaccagaaa gtgatcgatg
     9001 acctgatgaa agttctcttc tgtcatccac caggtaagca attcctgatc aagaaaattc
     9061 cagttcgatt gtggcatgac tcgggtacca attcgcggtc gcggtaaaac catccctttt
     9121 gccgttaacg ttttgaccgc ttcgcgtacc gctgtacgac tcactccaaa ttgctcgccc
     9181 agctcaatct caccaggcaa aatggtgccg ggttcatatt caccttttaa gatccgctgc
     9241 gccagctttt cagccagaac atacgaaagg tttttctgtg cagctaactg ttgtgcgctt
     9301 aaaggcatta ctaatcttcc tttttctttt tattcctcct tagtatgcca ccaggaagtg
     9361 tgattacggt tgcaaaaacg gcaaattgct tgttttatgg cacattaacg gggcttttgc
     9421 tgaaaaaatg cgcggtcaga aaattatttt aaatttcctc ttgtcaggcc ggaataactc
     9481 cctataatgc gccaccactg acacggaaca acggcaaaca cgccgccggg tcagcgggat
     9541 tctcctgaga attccggcag agaaagcaaa aataaatgct tgactctgta gcgggagagc
     9601 gtattatgca caccccgcgc cgctaagaaa aa
//
LOCUS       DACADF010000161         9623 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.163, whole genome shotgun sequence.
ACCESSION   DACADF010000161 DACADF010000000
VERSION     DACADF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.163"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            6..506
                     /gene="tssB"
                     /locus_tag="ISB30_004015"
     CDS             6..506
                     /gene="tssB"
                     /locus_tag="ISB30_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HAP1991597.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            565..2103
                     /gene="tssC"
                     /locus_tag="ISB30_004016"
     CDS             565..2103
                     /gene="tssC"
                     /locus_tag="ISB30_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HAP1991598.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDS
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDVSKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2121..3458
                     /gene="tssK"
                     /locus_tag="ISB30_004017"
     CDS             2121..3458
                     /gene="tssK"
                     /locus_tag="ISB30_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HAP1991599.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALTSGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLRALKNDSAIVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSIVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT"
     gene            3455..4120
                     /locus_tag="ISB30_004018"
     CDS             3455..4120
                     /locus_tag="ISB30_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HAP1991600.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     LVRERLKDAGFSRESVEHITYAQCALLDETVLSRGGMDDGQAIWMKDPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLVLAGVWWGLHYWLNVLVDELLPQ
                     SLR"
     gene            4133..5785
                     /locus_tag="ISB30_004019"
     CDS             4133..5785
                     /locus_tag="ISB30_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAP1991601.1"
                     /translation="MRNALKQTVLLWTLLLLLTTWLGFFAVSAVVKYAVVVILVAVTA
                     AVMAYWHKKKGQVVADDVWPSSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     PVSDEEQLVAQVERLLTLRPAWASQFAVAYTVMPGMYRDAAVLTGQLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEVQVATSAETTMPAQWIAQSGAQE
                     RSQRLCYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMTVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRRLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPAEEFITKARHLSVLKDDAVMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRAIRDWRPPEQKMDVTASLPVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            5843..6334
                     /locus_tag="ISB30_004020"
     CDS             5843..6334
                     /locus_tag="ISB30_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014070071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HAP1991602.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6525..9161
                     /gene="tssH"
                     /locus_tag="ISB30_004021"
     CDS             6525..9161
                     /gene="tssH"
                     /locus_tag="ISB30_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HAP1991603.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRPVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
                     RQPQVTEPLTSAQPGAATTDAPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     AQQIMTLRQEGTDSSELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLRD
                     EQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGRVIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLRDHFQPALMARFQTVIYRPLGQEAMRTIVEMKLAQVVR
                     RLHQHYGLETEINDSLYDQLTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAVHH
                     KPARLRLGWDEEDGIVLEFDEK"
     gene            9173..>9623
                     /locus_tag="ISB30_004022"
     CDS             9173..>9623
                     /locus_tag="ISB30_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAP1991604.1"
                     /translation="MNVQFFDHAHHKLKIRGLKSPVDVLTFTGHEQLSSPFRYDIEFT
                     STDKAIEPESVLMQDGAFSLSAPPVQGMPVQVPLRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKS"
BASE COUNT         2145 a         2712 c         2785 g         1981 t
ORIGIN      
        1 aggatatggc cgacagtttc cagaatgaag ttcccgccgc tcgtgtaaat atcaagcttg
       61 acctgcatac aggcaatgct aaaaagaaag ttgaactccc cctcaagctt cttgccgtag
      121 gcgattacag taacggaaaa gagcagcgtc cgctgtccga acgggacaag attgatatca
      181 ataaaaacaa cttcaacagc gtcatggctg agttttcgcc tgcggttaat ttaacagtag
      241 aagatacgct aaacggaaac ggtaatgaac aaaatatcgc gcttgaattt aaaagcctga
      301 aggattttga gcccgaacag gtagcaaaaa atattccaca acttcgtgta ttactcgcta
      361 tgcgcaattt actacgtgac ctgaaatcca atctgctgga taacgcaacg tttcgccgtg
      421 aactggaaaa tatcctgaaa gacccgacgc tcagttcaga actgcgtgat gaactggcga
      481 aaattgcgcc gcaggagaac gtttaacccg aacgcgtatt caggttaaac cggagaagaa
      541 aggatcactg aaaaggaaat gctgatgtct gtacaacaag aacatgccac ctctgaaact
      601 gcaacactca ccaccactga gtccggcggc gtttatcagt ccctgtttga taaaattaat
      661 ttaaccccgg tctcttccat tcaggaaatc gattcatggc aaaacagcga aacgctggcc
      721 gatgcctcac ccgatgagcg cgtgacggcg gctatccacg ttctgctttc ctgtctggcg
      781 aagtctggcg aggacgtggt taaactcgac aaaagcctgc tggattttca tatcgacgat
      841 ctggatcaga aaatcagtaa acagcttgat gcagtcatgc accatccgga attccagaaa
      901 gtcgagtcgc tctggcgcgg cacatggttc gtcgtacagc gcactgattt tcgcaaaaat
      961 gtcagaattg aactgctgga tgtcagtaaa gaacatctgc ggcaggactt tgatgattct
     1021 ccggaaatca ttcaaagtgg tttatatcgc catacataca ttcaggagta cgatacgccg
     1081 ggtggcgaac ctgtagcctc attaatatcc agctatgaat tcgataacag cccgcaggat
     1141 attgccctgc tgcgtaatat ttccagagtc tctgctgcat cacatatgcc ctttatcggt
     1201 tccgtcggac cgaagttctt ccttaaaaac tcgatggaag aagtcgccgc aattaaagat
     1261 atcggcaact attttgaccg cgcggaatat atcaaatgga agtcgttccg cgataccgac
     1321 gacagtcgct acgtgggatt agtgatgccg cgcgtgctgg gccgtctgcc ctatgggccg
     1381 gacacagtgc cggtacgcag ctttaactat gtggaagaag tcaaaggccc ggatcacgaa
     1441 aaatacctct ggacaaacgc ctcgttcgcc tttgccgcca atatggtgaa gagctttgtg
     1501 aataatggct ggtgcgtgca gatccgtggt ccgcaggcgg gtggcgcagt ggccgatctg
     1561 ccgatccatc tttacgatct cggcaccggc aatcaggtca aaattccgtc cgaagtgatg
     1621 atcccggaaa cccgcgaatt tgaatttgcc aaccttggct ttattccgct ctcttattat
     1681 aagaatcgcg attacgcctg cttcttctca gcgaactctg cccagaaacc ggcgttgtat
     1741 gataccgctg acgccaccgc caacagtcgt attaacgccc gtttgcccta tattttcctg
     1801 ctgtcccgca ttgcgcatta cctgaaaatc atccagcgcg agaatatcgg taccaccaaa
     1861 gaccgccgcg tactggaact ggagcttaac acctggatcc gcacactggt gacggagatg
     1921 accgatccgg gtgatgaact gcaggcgtct cacccgctgc gcgacggcaa ggttatcgtg
     1981 gaagatattg aggacaatcc gggcttcttc cgcgtcagac tctttgccgt gccgcatttc
     2041 cagattgaag ggatggatat caacctgtca ctggtttccc agatgccgaa agcgaaagcc
     2101 tgaccgggcc gtggtgtgat atgaaaattc atcgaccact gtgggctgag gggacattcc
     2161 tgtcctctca gcagtttcag cagcaggccc gctgggaagc gttcagcaat gacagcattg
     2221 cgcaactttg tattcgtcat ccgtggggga tcgcgaatgt gctctttgac cgcgatgccc
     2281 tgacgtccgg aaagctgaaa acgcaggctg tacgtctgcg ttttgctgat ggcacgctga
     2341 tcgattcaga cgtgagtgac gttctgccgc tcgcctgcga tctgcgcgcg ctgaagaacg
     2401 acagcgcgat tgtcctgctg gcgctgccgc ttgcgcatgg taacggcggt aatctcggtc
     2461 agggtgagca gactgaaaga ccgctgcgct atcgtcagga atggcaaaag gtgcaggata
     2521 tctacggcag tgacagtgaa gatatggccg ttgagcgtca tgcgctgtcc ctgcgctttg
     2581 ctcatgataa caatcaggat tacatcacct gcccactggc gcgtctggtg cgggatgttc
     2641 agggcaactg gacacaggat gagagctata ttccgccgtt actggcgttt aacgcccatg
     2701 acgggctggt acagcgtctg gacacgctgc tgttgcagct acgggcgaag tgccagcgtc
     2761 tgatggcgat gcgccgggaa agcaaccagc gtatggcgga cttcgctgtc gccgatgtct
     2821 ccctgttctg gctgcttaac gcgctgaact ccgccgagcc ggtactgagt gatttcctgc
     2881 gctacccggc ggttcatccc gaactggtct ggcgggagct ggcgaggctt gccggtgcgc
     2941 tgctgacgtt ttcccttgaa cataacgtca gcgccgttcc gccctatgtc cacgaatcgc
     3001 cgtccatcgt ctttccgcca cttttctccc tgctcagcga actgctggaa gccagcctgc
     3061 cgtcacgggt gatcgcgctg gatctggcgt ccctgccggg caaccgctgg aaagcagacc
     3121 tgcacgatcc gcgcctgcgg gaagaggccg acttttacct gtccgttcgt tcctctctgc
     3181 ctgcgcatca ggtactgcac cagctaccgc tggtctgcaa aatcggtgcg cctgatgacg
     3241 tcacgctgct tatcaatgtg gcgctgaatg gtgttccact ggtgccgctt acctcagttc
     3301 cggcagcgct gccactgcgg ctggagaacc agtattttgc gctggatatg cacagtgacg
     3361 cggctaaatc gatgctggag tccgggtgct gcatgattta tgcgccggga acaatgggcg
     3421 acctgaaacc tgaactgttt gcggtgctgc gcacatgaaa aaagagacgg atatcaatat
     3481 cgacgcgctg ctgcgcgaca cgtttctgac ggtggtggaa ctgcgtcagg gaaccaccgt
     3541 gcgtcacggt atggagctgt atcgccactg ccagcggcag gtggaactgg tccgcgaacg
     3601 cctgaaagat gccgggttca gccgggagag cgtggagcac attacttacg cgcagtgcgc
     3661 cctgctggac gaaacggtcc tgagccgtgg cggcatggac gacggtcagg ccatctggat
     3721 gaaggatcca ctccagtcgc atttcttcaa cactctgcag gccggtgagc tgctgtacga
     3781 gcggatgaag caggtgctac aggagcccgc ccccgcgcag gccgtgctga cctgctttca
     3841 ccgtgtcctg ctgctgggat ttcgcgggcg ttatcaggat ccggcagccc cggagcgcga
     3901 ccagcttatt tcgacgctca acgggcaggt cgcgccattt ggtgtgctgc cggaaacggc
     3961 ggtactgaac gtgccgttat ccccccgtca gcaccctttg ctgcattcgc cgtttttctg
     4021 gctggtgacg ctcacactgg tactggccgg tgtctggtgg gggctacatt actggctgaa
     4081 cgtgctggtg gatgaactgc tgccgcaaag tctgcgttaa agggagtcgt gagtgaggaa
     4141 tgcgctgaaa cagacggttt tgttatggac tctgttgttg ctgctgacta cctggctggg
     4201 tttctttgct gtatctgcgg tcgtgaagta tgccgtggta gtgattcttg tcgccgtcac
     4261 agcggcggtg atggcgtact ggcataagaa aaagggacag gtcgtagcgg acgacgtatg
     4321 gccgtcatcg ctaccgccgg aaacctaccg acagccggta gtgctggtct gtggcgatat
     4381 gtcggcgcac ctgttcactg acagcccggt ccggcaggtt tcagaagggc tgtatctgcc
     4441 tgtttctgat gaagaacagc ttgtggcgca ggtggagcga ttactgaccc ttcgcccggc
     4501 gtgggcatcg cagtttgccg tggcgtatac cgtcatgccc ggcatgtacc gggatgcggc
     4561 ggttctgacc ggacaactgc gccggttcgc tcacagtatg gcgacggtgc gtcgccgggc
     4621 gggcgtaaac gtaccctggc ttctctggag cgggctgtcc ggctcgccgt tgccggagag
     4681 agcgaattca ccgtggttta tctgtaccgg cggcgaagtt caggtagcaa catccgcaga
     4741 gaccaccatg cccgcgcagt ggattgcaca atccggcgca caggaacgca gtcagcgact
     4801 ctgttacctg ctgaaagccg aaagcctgat gcagtggctg gatctgaatg tgctggcgga
     4861 actgaacggc ccggaggcga aatgtccacc actggcgatg accgttgggc tggtcccctc
     4921 gttgcctgcg gtggataaca acctgtggca gttgtggatc accgccagaa ccggcctgac
     4981 gccggatatc gcggacaccg gcacagacga tgcgctgcca ttcccggatg ccctgttacg
     5041 gcggttgccg cgtcagtcgg gctttacccc gctgcgacgg gcctgcgtga ccatgctggg
     5101 cgtcaccacc gtggcgggta tcgccgcgct gtgcctgtca gccacggcaa atcgccagtt
     5161 attacggcag gtcggtgacg atctgcaccg gttttatgcc gtcccggcgg aagaatttat
     5221 caccaaagcc cgtcacctgt cggtgctgaa agacgatgcg gtcatgctcg atgggtatta
     5281 ccgggaagga gaaccactac gtctcggtct ggggttatac cccggtgaac gcatccgcca
     5341 gccggtatta cgcgccattc gcgactggcg tccgcctgag caaaaaatgg acgtgacggc
     5401 ttcgcttccg gttcagaccg tgcgtcttga cagtatgtcg ctgtttgacg tcggacaggc
     5461 ccgcctgaaa gacggctcga caaaagtgct ggtggatgcg ctggtgaaca tccgggcaaa
     5521 accgggctgg ctgatcctcg tggccggata taccgatgcc accggcgatg aaaaaagcaa
     5581 tcagcagtta tcgctgcggc gtgccgaagc ggtgcgcaac tggatgctgc agaccagcga
     5641 catcccggcc acctgttttg ccgtacaggg actgggcgag agccagcctg cggcgaccaa
     5701 cgacacgcca cagggccggg cagtcaaccg gcgtgtcgaa atcagtcttg ttccgcgttc
     5761 tgacgcctgt caggacgtga aataaaacat accgccggaa gaaggcggtg cttcaatcac
     5821 actaacaagg agagtaattc tcatggctat tcctgcttat ctctggctga aagatgacgg
     5881 cggcgcggat atcaaaggtt ccgtggacgt tcaggggcgc gaaggtagca tcgaagtggt
     5941 ggcgctggat cacgatgtgt acatcccgac cgacaacaac accggcaaac tgaccggtac
     6001 ccgtactcac aagcccttta cgtttaccaa agaaatcgat gcctccagcc cgtatctcta
     6061 caaagctgtg accaccggcc agactctgaa aacggcagaa tttaagtttt accgcatcaa
     6121 cgatgccggt caggaagtgg agtacttcaa catcacgctt gataacgtca agctggtcag
     6181 agtcgctccg cttatgcacg acatcaagga tccttccaga gagaagcata accacctgga
     6241 acgtattgag ttccgctacg agaaaatcac ctggacttac aaagacggca acatcattca
     6301 ttccgactcg tggaatgagc gtccttccgc ctgataactg cggcagacag gaaactgtct
     6361 gcctttttgt tgtgtgcttt gtccagtgcc gcaggcaccg gtactgagca aagcataccc
     6421 acaatctggt gaaacctgat gggcctgagt gaggtaacgg ctatgggcgg tagcgtgcct
     6481 gaacgcggat cgcaggaata cagaacacga taaggaaacc ttctatgacc ggaaatcacc
     6541 ccgccgcgct gctgcgtcgc cttaacccat actgtgcacg ggcgctggac gctgccgcct
     6601 cactgtgtca gacccgcgcc catgcggaaa taaccattga acactggctg ctgaaactgc
     6661 tggagcaggg agaaggcgat atcacggtga ttgcccgccg ctatgaatgg gatatcgaca
     6721 cgctctggca gtctctgctg gcacatctgg acaccttacc ccgcccggtc cgcgaacgtc
     6781 ctcaactttc tgaaccgctg gcagcgctta tccgacaggc gtggctgata gcgtcactgg
     6841 aaggcgacga tccacaaatc cgcagccagc atctgctgat ggcgctgaca gaaaaaccga
     6901 tgctgcccgc ctgtaatgac ctgtgggtat tgctgagcct gagccgtgtg cagcttgagc
     6961 ggctgcgtcc cctgctggat gcgcagtcgg atgaatgtcc ggcacgtcag ccacaggtca
     7021 ccgaaccgct gacatccgcg caacccgggg cggcaacgac ggacgcaccg gcaaaaacgc
     7081 tgacggggaa acaggatgac gccctgctgg cggtgcttaa ccgctttacc gaagacgtga
     7141 cggaaaaagc ccgcagcggg cgaatcgacc cggtattcgg gcgcgacacg gaaattcgcc
     7201 agatggtcga tatcctctcc cgtcgccgca aaaacaaccc gattctggtg ggagaaccgg
     7261 gggtgggcaa aaccgcgctg gtggaagggc tggcgctgcg tatcaccgaa ggcaacgtgc
     7321 cggacagcct gaaaacggtg catatccgca cactggacct cggtctgtta caggctggcg
     7381 cgggcgttaa aggtgaattt gaacagcggc tgaaaaatgt catcgatgca gtgcagaaat
     7441 caccggagcc ggtactgctg tttattgatg aagcccatac cattatcggt gcgggtaatc
     7501 aggcaggcgg cgcggatgcg gcgaacctgc tgaaaccggc gctggcaagg ggcgaactgc
     7561 gcaccatcgc ggcgaccacg tggagcgaat acaaacagta ttttgagcgc gacgccgcgc
     7621 tggaacgccg cttccagatg gtgaaagtcg acgagccgga tgatgacacc gcctgcctga
     7681 tgctgcgggg actcaaggcc cgctatgcac agcaccacgg cgtacatatg ctggacagcg
     7741 ccattcagac cgccgtgcgc ctgtcgcgcc gctatctgac cggacgccag ttgccggaca
     7801 aagcggttga tttgctggat accgccgggg caagagtccg catgagcctt gacactctgc
     7861 cggagccgtt gacgcagctt catgcgcgac tggcggcgct ggatatcgag cgggaggcga
     7921 ttgagcagga cagcgtgttt tatcccgaaa ccagcccgga gcggctggcg gaactgaccg
     7981 atttgcgtga tgagctacag gcagaagccg ggcatctgga agcgcagtat cagcaggaaa
     8041 aggcactggc gcagcagatt atgacgttgc gtcaggaagg aacagacagc tctgaactgc
     8101 aacagcaact gcggacgcat cagggctttg caccgctgct ggcgctggat gtggacgccc
     8161 gcgccgtcgc cacggtggtg gcggactgga ccggcatccc gctctcttcc ctgctcaggg
     8221 acgagcagag cgacctgctc agtatggaac agagtcttga aaaccgcgtt gtcgggcaaa
     8281 gcccggcgct ctgcgccatc gcacagcggc tgcgggcggc taaaaccggc ctcacgccgg
     8341 agaacggccc acagggggta ttcctgctga ccggccccag cggcaccggc aaaaccgaaa
     8401 ctgcgctcac actggccgac accctgtttg gtggtgaaaa atcccttatc accattaatc
     8461 tttcggaata ccaggaaccg cataccgttt cccagttgaa aggctcgcct ccaggctacg
     8521 ttggttacgg tcagggtggc gtacttaccg aagccgttcg caaacgccct tacagcgtgg
     8581 tgctgctcga cgaagtggaa aaggcgcatc gcgacgtgat gaacctgttc tatcaggtgt
     8641 tcgaccgggg ctttatgcgc gacggcgaag ggcgggtaat cgacttccgc aacaccgtca
     8701 ttctgatgac cgccaatctg ggcagcgacc acatcatgca actgctggag gaaaaaccgg
     8761 acgccacgga cgcagacctg catgaactgc tgtatcccct tctgcgagac cattttcagc
     8821 ctgcactgat ggcgcgtttt cagacggtga tttaccgccc gctgggacag gaggcgatgc
     8881 gcaccattgt ggaaatgaaa ctggcgcagg tggtccgccg tcttcaccag cactacggac
     8941 tggaaacgga aatcaacgac agcctgtatg accagcttac cgccgcctgc ctgctgccgg
     9001 ataccggggc gcgcaatatc gacagcctcc tgaaccagca aatcctgccg gtcttaagcc
     9061 agcagttgct ggcacagcag gccgtgcatc ataagcctgc ccgactgcgg cttggctggg
     9121 atgaggagga cgggattgta ctggaatttg atgagaaata atggaggctc ttatgaacgt
     9181 acagttcttc gaccacgcac accacaagct caaaattcgc ggcctgaaaa gcccggtgga
     9241 cgtgctgact tttacaggcc acgaacagct cagctcaccc ttccgctacg acattgagtt
     9301 taccagtacc gataaagcca tcgagccgga gtcggtactg atgcaggacg gcgcattcag
     9361 cctgagcgcc ccgccggtac aggggatgcc ggtgcaggtc cccctgcgca ccctgcacgg
     9421 cgtcattacc ggctttaaac acctctcctc gtcacaggac gaagcccgtt acgaagtgcg
     9481 ccttgaaccc cgtatggcgc tgcttacccg cagccgccag aacgccatct accagaacca
     9541 gacggtgccg caaatcgtcg aaaaaatcct gcgcgagcgg caccagatgc gcggtcagga
     9601 cttcgtgttt aacctcaaaa gcg
//
LOCUS       DACADF010000162         9566 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.164, whole genome shotgun sequence.
ACCESSION   DACADF010000162 DACADF010000000
VERSION     DACADF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.164"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(306..381)
                     /locus_tag="ISB30_004023"
     tRNA            complement(306..381)
                     /locus_tag="ISB30_004023"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(346..348),aa:Asn,seq:gtt)
     gene            complement(482..2125)
                     /gene="yeeO"
                     /locus_tag="ISB30_004024"
     CDS             complement(482..2125)
                     /gene="yeeO"
                     /locus_tag="ISB30_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HAP1991605.1"
                     /translation="MLRHILTAKNILSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSSFEMWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTDVKALALTYLEL
                     TVFSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWAGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            2130..2205
                     /locus_tag="ISB30_004025"
     tRNA            2130..2205
                     /locus_tag="ISB30_004025"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2163..2165,aa:Asn,seq:gtt)
     gene            complement(2243..3193)
                     /gene="cbl"
                     /locus_tag="ISB30_004026"
     CDS             complement(2243..3193)
                     /gene="cbl"
                     /locus_tag="ISB30_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAP1991606.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3295..4212)
                     /gene="nac"
                     /locus_tag="ISB30_004027"
     CDS             complement(3295..4212)
                     /gene="nac"
                     /locus_tag="ISB30_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAP1991607.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTGAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4539..4614
                     /locus_tag="ISB30_004028"
     tRNA            4539..4614
                     /locus_tag="ISB30_004028"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4572..4574,aa:Asn,seq:gtt)
     gene            complement(4670..5605)
                     /gene="ldtA"
                     /locus_tag="ISB30_004029"
     CDS             complement(4670..5605)
                     /gene="ldtA"
                     /locus_tag="ISB30_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAP1991608.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGKLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5667..6746)
                     /gene="cobT"
                     /locus_tag="ISB30_004030"
     CDS             complement(5667..6746)
                     /gene="cobT"
                     /locus_tag="ISB30_004030"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAP1991609.1"
                     /translation="MQILADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6758..7501)
                     /gene="cobS"
                     /locus_tag="ISB30_004031"
     CDS             complement(6758..7501)
                     /gene="cobS"
                     /locus_tag="ISB30_004031"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAP1991610.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIEFGELVFLLALL"
     gene            complement(7498..8043)
                     /gene="cobU"
                     /locus_tag="ISB30_004032"
     CDS             complement(7498..8043)
                     /gene="cobU"
                     /locus_tag="ISB30_004032"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAP1991611.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2657 a         2521 c         2029 g         2359 t
ORIGIN      
        1 tctgattacc gaagacaaag tggcaattac gtatagtttc ccgataaaga cgcgacagct
       61 acatcccgca taaggcattt ttctctttat ctttgtacgg tacttcatgg aacagaattt
      121 ttgaccttgt gaatcgtgat gtctgttggg gaggggcaat ttgctcactg aagcgtgaga
      181 ctcgattaag cgcacgaaac acagaaatca aaaacccggc cactttttta caaagtgacc
      241 gggtaaaaat aatttttatt ttttaactgt cctgagactc atagagatgt ctcaaaacta
      301 aaatttggct cctctgactg gactcgaacc agtgacatac ggattaacag tccgccgttc
      361 taccgactga actacagagg aatcgtgaga acgaggcgaa tattagcgat gcccacccac
      421 aatgtcaaag cctgtttttt aaatttgaaa tcgtttgctg aaataatctg cattttgtcg
      481 tttattccga cacaactggc tttttttcac acttttgcgg ctcggatcga gggtatttcc
      541 atagccaacg tccagtaacc attcgccagt aaaacagcac ggcccgcaca gcccagtcgg
      601 caaacattcc catccagaca ccaaccacac cccagccaag catgattccc agcacataac
      661 cgactacaac ccgacaaccc cacatgctca acatcgaaac ccacatggcg taacgggcat
      721 cacgagcacc tttaaatcca gcgggtagca cccatgaggc ggaccaaata ggcataaata
      781 aagcatttag ccaaatcaga atcacaacga cgtgtttaac ctgtggatcc tgggtgtaaa
      841 acgatgccat aaccccggca aagggagccg ttagccaggc gatggccgtt aatccaagag
      901 tggacagcca gaacacatgc cgcaactgaa tctctgcttg cgctatctgc cctaccccca
      961 accttcggcc tgtaatgatc gtagaagcag aaccgagcgc acttccgggt aagttgataa
     1021 gagccgcaat tgagaacgcg ataaaatttc cggcaataac gctggtcccc atcccggcaa
     1081 cgaacatttg ggttaataac cgaccactgg taaataacac tgattcgaca ctcgctggaa
     1141 taccaatccc catgacttcc cagataatgc taaaattcag cggtttaaaa tagctcttta
     1201 acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac ccacaaaatt gcaactgcgc
     1261 caatataacg agaaatggtt aaacccaacc cagccccgac aaatcccagt cccgcccagg
     1321 agaaaaggcc gtaaatcaat atgccgctaa taataatatt aagaatattc aggctaccgt
     1381 taatcagtag cggtattttc gtattccctg caccacgaag tgccccgcta ccaataagag
     1441 tgatggcagc tgctggataa ctgaatacgg tcagctccag ataagtcaac gccagtgctt
     1501 taacgtctgt cgtggcatca cccgcgacga aatcaataat ttgttcgcca aaatgatgaa
     1561 ttagcgttgc caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca
     1621 ccctcgctcg tcgtcgatct cgcttaccga gactaaatgc tacaacgaca gtagtaccaa
     1681 gatcgatagc tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc aaccccacgc
     1741 cggccatcgc atcttttccc agccagctga ccagaaaagt gctcaggacc cccatcaaca
     1801 ggacacaggc gttctccatg aagataggaa cagcaagcgg ggttatctcg cgccagaaca
     1861 acactttgta gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc tggcgtaaag
     1921 cagaggagat attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaatgatg
     1981 agggagaatc agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa
     2041 ctgttgatag aaacggcaaa cagttgggaa atttaaaaat cgggtttgac aaaatatttt
     2101 tcgccgttaa gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga
     2161 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc
     2221 cgcttttata gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttttcca
     2281 tcacctggcg cttgatatcc tcaactgaca gtcctgcatt acaaagttcc agaaagcgcc
     2341 agacatagtt acgctgaagt tgccctcgct tcagtcccaa ccagacagta ttagcatcga
     2401 aaagatgccg cgtatccagg cggattaaat tcttttcctc ttgttcgccg ctggattgtt
     2461 cggcaactaa tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct
     2521 gtgcacttaa tacaatatct gccggcaaac ctttgcgggc aaatgcgtca tcaatgcgtg
     2581 agcgccccgt aatcccctgt cggtaagtaa ttaacggcca cttcgctatt gattccagcg
     2641 tcaacggtgt aatttgcgtc aagggatgat cgagtggaac aagcaaacta tggtgccaac
     2701 gaaaccacgg gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa
     2761 tatcagcttc gccattttgc aacaacgtcg caatttcctg tggtgtaccc tggattagct
     2821 ccagccgaac ctccgggaaa agttcgcgaa aagctttaat gacctcaggc aagctataac
     2881 gtgcctgagt atgcgtcgtt gcaatagtga gaacgccaga cgtatcgttg gtaaacaggt
     2941 ctgcgagccg acgaacatta ctggcttcat tcagaatgcg ttctgcaatg accagtaatg
     3001 ctttgcccgg ttcagtcatg cccagcagtc gcttacctcg tcgaacaaat atttcgatgc
     3061 caagttcatc ctccagttcc cgaatatgac ggctaacgcc tgactgtgag gtaaaaagca
     3121 tattcgcaac ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt atctttagtt
     3181 gttggaaatt cacggtaaac tccgggcagt tcagatatcc cgttattgtt aaagtctaat
     3241 gcccggcata acaaataata aaaacccgca tcttattcca tcccgatata acgcttagct
     3301 caccaattgc cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc
     3361 tttcactgcc tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta aggacaaaga
     3421 gagactcatt gaaggagtgg taatgcgtga catccaccca tttactgcgc cacataacga
     3481 acgcgcagcc gattcaggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag
     3541 cgtggcaata gactcaattt caccaatgac ttttgccgtg agccgccgta gggaaaaagc
     3601 ctcatcaaca cgaagtctaa cagcgctgta atcactgggg agaaaaaggt tcatttgcgc
     3661 aatagcatta acatcaacgc tttgccccgg gcaatcttga gttcctacca ggaaaagatc
     3721 ttctttcagc aaagcctgac tggatacccc agccacaggg gaatgctcat aaatcaccgc
     3781 catatcgagt tggtgattta tcagtttttc gttaagcact gcaccactat tttcatgaag
     3841 atagataacg acctccggga attcagcgcg aaccgcctgt aataagggca tggtgatgga
     3901 tgacgcagcg gttcctggcg caaagccaat cgagacttgc cccgataatg actgaccaac
     3961 gttatgcacc gccagttggg cctgctcaca ctgacgtaaa atggcccgtg catgggtata
     4021 gagaattttt ccggcgcctg ttggcgtaac gccccgtttt gtgcgaatca aaagctgttg
     4081 atttaactca ccttccagtg tggcaacctg ctggctgagc gctggttgtg cgatatgcaa
     4141 tacttcagca gcctgggtca ggctaccaat atctacaatt tttacgaagt atttcaggcg
     4201 tctgaagttc atgttgcctc cggtttttaa gaatcggccc aagtgccgcc attacttaca
     4261 accagaatgc aagatgcttg ccagttctat tttagcgttg atgcgcaagc taaccaactg
     4321 tcaaataaga gattatgata gattcgtcat tggccctttt tatcagcagt cgcattcacc
     4381 tgccctataa aaggggggta tgcaccacga tggttcatta cccaataaga ttgaaagctc
     4441 accactttgt tgaaattgtc agcaaacaaa caaaaaaatg catttcaccc tttgacatca
     4501 ccatgcactg ccattaatat gcgccccgtt cacacgattc ctctgtagtt cagtcggtag
     4561 aacggcggac tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattca
     4621 aaaaagcctg ctttcgagca ggctttttac tttctaatta ccaacgctct taaaacatct
     4681 gtcttgaacc agaactaatt tgcacaggca ttcccgatcg atgttgcaac gcagcatttg
     4741 cgcgatttac atcaacttct tgcccgttga taaacgcccg caaagatggg gttaccggca
     4801 atggcacttt tcggtcagac tcatattctg cacgattgcg cgacagcggc tcatgaactt
     4861 ccagccactt tgaaccatcc ggttccgttg tgtatttcac tggctgatca ataatttgta
     4921 cacgagtgcc tacaggaaca ttatcaaaca gatatttgat atcgtcatta cgcagacgaa
     4981 tacagccctg acttacgcgg agcccaatac caaaattggc attggtaccg tggatggcat
     5041 acaacttgcc aatataaatc gcgtacagcc ccatggggtt atcgggcccc gcaggaacaa
     5101 atgcgggcaa actctcccct cgtttcgcat attcgcgccg ggtgttcggc gttggcgtcc
     5161 aggttggtgc ttcttgttta cgttcaacgg tagtcaccca gttacgcggg gtttctcgcc
     5221 cagcctggcc gataccaata ggaaagactt ccactgtatt actgtctggc ggatagtaat
     5281 aaagacgcat ttcagcgacg ttaaccacaa tccctttacg aacagtggcg ggcaaaatca
     5341 gttgctgcgg aatggtgagt tgtgagccag gcttcggcaa aaaaacatca gcgcccggat
     5401 tcgcttccag catattactt aacccttgcc cgtattgtgc ggcaaaagtc tccagcggct
     5461 gagtattgtg atcaggaaca gttacagtaa gcgactgccc cactaaacgg ctaccctctg
     5521 gaggtaatgg ataagttacc gccaggctat tttggctggc aaaaagcaga gcaaatgagc
     5581 aaagaatatt tacacgacgc atcatgtccc tttcctaagt cgcgaatact atccgataag
     5641 tatagctttt atcagacttt tcgtttttag ctgttcaaat cagaagtcgt attccccggt
     5701 agaacaatat tactggcagc aagttcgccc atgttgttgt atatcgcaca agcagcttcg
     5761 atgatggaca tcgccagagc tgcaccactc ccctcaccta aacgcatatc catattgaga
     5821 taaggctcca gccccaaatg cgagagcgct atacgcgcgc ctttttctgc cgacaagtga
     5881 gaaggaatga gatacggttt gattgcagga gacatctggc aggctgcgag cgcagcagca
     5941 taagaaagaa atccatccag caacacgggt aaaccgcagg aagcagcacc taacatcact
     6001 ccagctattc cgaccaaatc aaatccaccc acttttgcca ggacatcaac accatcctga
     6061 ggatttggtt gattcaacgt aatcgcccga cgcacaacat caattttatt agccagttta
     6121 tctgtcggca ggtttgcgcc aatcccaacc acttcttcag gatcccggcc agtgattgtg
     6181 ctgactattg ccgctgccgg tgtcgtgttt gccatcccca gttcacctac accaaacagc
     6241 gtgacaccgt tttttgccag ctcccgcgta taacatatga cgtccaaaag caacttttca
     6301 gcctgacggc gactcattgc cggagctgaa gcaatattgc cgctacctcg tgcgacacgc
     6361 atgttgataa gcccggggat aggctcagca gtatcaatac caacatcaat tacgtggacg
     6421 ttagcgcccg cttgtgctgc cagcacacac acgccggttg ttccacgggt catattttca
     6481 gcctgtatgg ctgtcacttc ttttggagaa atagcgaccc cttcctccca gacgccgtga
     6541 tcggcacaca taaccagtac tgcttttttg cccacatgcg gtatgccatt caaccccggc
     6601 attcctgcca gttgtatggc aagcgcctcc agctttccca ggctaccaac aggtttgagt
     6661 aacccgtcaa catgccgttg tgcacgcgac atagcggcag aattgatggc tgggatcgta
     6721 ttcagtaaat cggcaagtat ttgcatgtca cgtctcttta tagcagtgcc agcaggaaaa
     6781 ccagttcacc aaactctatg gctgctccca gcgtatcgcc cgtttgcccg cccagcgtac
     6841 gtttgagaag ctgaccgagg atgaaaatcg ccaccatcgt caccaccata gcagccacac
     6901 catgcatacc cggcaataat acggcagcaa aaatagcggc taagccgagt gtgacgcagg
     6961 tttgtcgccc atcaattttg ccaatgaaca cattgccaag cccttcttcc cgcgcataac
     7021 gatgacgata catcaataat gcggcagtcc cgcggctgac cgcacatgct gccgccagcg
     7081 atgcaaggat cggttcgcca cgcagagcca actcgcttaa caccagaatc tttgccagta
     7141 ccacaaaaat caatgccaga ccgccgtggg tgcctaaacg gctatcacgc atgatttcca
     7201 acatgcgatc gcggctacgt gcagaaaata cgccatcaca ggtatcagcg aggccgtcca
     7261 gatggaatcc cccggtcatc agcgccagca caagtacgct aaacagtgcc gccagtggta
     7321 cgccacacca tgcctgcagc accatgaaga ccagcccgct aatcgcgcca agcaataatc
     7381 caatcaaagg aaaagtaata ataccgcgag aataatgctc gaaatccagt ccctgggacc
     7441 agcgacgcgg tacaggcaga cgcgtaataa atgagagcat cgcccaaaat aatttactca
     7501 tttgattttt actccaatac ccgaaaccac cagccatact tcatttgccg cagcggccaa
     7561 ctgctgattt acccgcccgg caatatcacg aaaatgtcgt gccagacgac tctccggcac
     7621 aatccccatt cccacttcgt tagtcactaa tacaaccttt gcggggcaac gttggcaggc
     7681 agcaatcaac gactgaatct cagcattaat cgcctgttcc atcgcctgat aatcccattc
     7741 atcagggtct ttatcgccgc cataatcaaa caacagatta gtcaccattg tggtaacgca
     7801 ttccagcaac acagcctcat tcgggttaat gtctgcatga attaattcat caagatgttg
     7861 ccagcgctcc actgtgcgcc agtgctccgg gcggctttgc cgatgatgtt ctatccgtgc
     7921 agccatctca tcatcaagga tttgcgaggt agcgatatac agaacctgtg aagagtcccc
     7981 aataagcacc tctgcgtggc gactcttccc gctccgtgcg cctcccgtca ccaaaatcat
     8041 cataccgact cctgattcaa atgtatgatg gtattcattg aacagagcaa tggatccccc
     8101 accgttgctg accgcaaaac atttacgcga ttaatactgc gcgtaatata attaaaaatc
     8161 cagcattctc aatccattac gaaataagat ggcattacgg aatagccaga ctttctcgtg
     8221 ttaacgtccg acaaaatgcg tcattgacag cggtttctgt ggattgacac atttcattca
     8281 gataatgaat taatgctact gccggaccaa ccagcaaacg ggcattgtgt tctgaaatcc
     8341 aggctattga ttcaacgtca gcgacagatc tgcgctgtaa aagaagttgt aaaacaacgc
     8401 aataaaatta tccggaatgg tggcgactat gcactagaac accacaattt cgctctctcg
     8461 gtaaggcact gtcacagcat tcaacagaat gtgacttgcc agatttatta gcgccaccag
     8521 atgtttaacc gggtaaccac gacccagtcg gggatatatt ccatcttctc gctgacatca
     8581 tcaagaataa aaaggtttat cacactcaga gcaacgttct tcagcaggcg ggataattct
     8641 tcaacattta aaagatgtgt tattaaaggc atacaaactg gacgtattgt attttctttt
     8701 ggtggttgac ctaaggtagc agtttatcct gatgcgctga gatttctgta atatctacgt
     8761 caacattctc ctcgaataga aaatgcagcc tcggcaagtg attcacattt tttaacaaag
     8821 cgttgttagc gtgccatttt cagaagctct ccaagaaagc gaattctttt ctcaaattct
     8881 gcattctcag acacagcttt ctactccatg gcaatggccc aaatacgaag ttgctcagga
     8941 tcattagtgg tgctgagaag ttaaataacc atgcggagta caactcgtca tcacgtattt
     9001 atatatcatg aagctattgt tgtgttttgt acatcagtag atgtattctg atatactcct
     9061 ttttctagac ataacctttc acctgcttgc aaagcttctg tgttctgaca ttgccaaatt
     9121 gttgcaattc tgtatccagc cttctttcag tcatagcttc gggccgcgat aagactcact
     9181 gatctgaccc tgattcctct tgcagacttt atagaccaat taaaatgcag tttctgcagg
     9241 tcaacgtctg accatcattg tcatcactct ggccattaga gtaaccttct gcattcatcc
     9301 ttttgtaaaa agtttatgtc agtatcagca attaaccgga cctgatactg atatgagtct
     9361 taccgcatat acggtcaatt tcagcaatta attacattat ccacgccaaa gtgtttgtca
     9421 tcgcaatgat ggtaccttct ttcagacacc attttttcaa ctccgttttc cacggaccgc
     9481 actcttatgt caagagtacg gtccgtggat acaaccagag accgactgac acgagtcaga
     9541 ggaaacgacg gatatgttca gtcgta
//
LOCUS       DACADF010000163         9391 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.165, whole genome shotgun sequence.
ACCESSION   DACADF010000163 DACADF010000000
VERSION     DACADF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.165"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            241..1539
                     /gene="kgtP"
                     /locus_tag="ISB30_004033"
     CDS             241..1539
                     /gene="kgtP"
                     /locus_tag="ISB30_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAP1991612.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAVLREWGWRIPFALGAVLAVVALRLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPIIGALSDKIGRRTSMLCFGSLATIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAFANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1536..1859)
                     /locus_tag="ISB30_004034"
     CDS             complement(1536..1859)
                     /locus_tag="ISB30_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAP1991613.1"
                     /translation="MRILFVCSLLLLSGCSHMANDNWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(1905..3260)
                     /gene="pssA"
                     /locus_tag="ISB30_004035"
     CDS             complement(1905..3260)
                     /gene="pssA"
                     /locus_tag="ISB30_004035"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAP1991614.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVNVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWILITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELDLIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3374..6034)
                     /gene="pat"
                     /locus_tag="ISB30_004036"
     CDS             complement(3374..6034)
                     /gene="pat"
                     /locus_tag="ISB30_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAP1991615.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIANLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLSLAQREES"
     gene            complement(6066..6764)
                     /locus_tag="ISB30_004037"
     CDS             complement(6066..6764)
                     /locus_tag="ISB30_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAP1991616.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSHFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPADKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTRAATGLGEHFTRFKTRYLAGKT
                     QHPGSITAEQLESV"
     gene            complement(6833..7252)
                     /gene="trxC"
                     /locus_tag="ISB30_004038"
     CDS             complement(6833..7252)
                     /gene="trxC"
                     /locus_tag="ISB30_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAP1991617.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7459..8496
                     /locus_tag="ISB30_004039"
     CDS             7459..8496
                     /locus_tag="ISB30_004039"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAP1991618.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQHRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8544..9233)
                     /gene="ung"
                     /locus_tag="ISB30_004040"
     CDS             complement(8544..9233)
                     /gene="ung"
                     /locus_tag="ISB30_004040"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP1991619.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
BASE COUNT         2300 a         2385 c         2428 g         2278 t
ORIGIN      
        1 ttatactccc acataagcca gattcaacag cgaatacgtc ttccccaaat tgcccacttc
       61 catacttcct ccttaccaga aatctatcct taagctcctt aataaccatt ttcctgctaa
      121 ctaaattcat ggttaaggtt gtataatgat gcgcaacaaa tgtataacat tttctttacc
      181 aaaaaaaata aacaaaagcg accgacaaaa gcatcggatt acggcaggag acataatggc
      241 atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac tcgtcgccgc
      301 atttgggcaa ttgttggggc ctcttcaggt aatctggtcg agtggttcga tttctatgtc
      361 tactcgttct gttcactcta cttcgcccac atcttcttcc cttccgggaa cacgacgact
      421 caactactgc aaacagcagg tgtttttgct gcaggatttc tgatgcgccc aataggcggt
      481 tggctgtttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct gttatcggtg
      541 tgtatgatgt gtttcggctc actggttatc gcctgtttgc caggctacga aacaatcggt
      601 acctgggctc cggcattatt gcttctcgcg cgtttattcc agggattatc cgttggcgga
      661 gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg caaaggtttt
      721 tacgcatcat ttcagtatgt gacgttgatc ggcgggcaac tgctagctct actggttgtc
      781 gtggttttac aacacaccat ggaagacgct gtactcagag agtggggatg gcgtattcct
      841 ttcgcgttag gagctgtgtt agctgttgtg gcgttgcggt tacgtcgtca gttagatgaa
      901 acttcacaac aagaaacacg cgctttaaaa gaagctggat ctctgaaagg attatggcgc
      961 aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct ttgtttctat
     1021 accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca tgccaacgtg
     1081 gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc aattattggc
     1141 gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc tctggcaacc
     1201 atttttaccg ttcctattct ctcagcattg cagaacgttt cctcgcctta tgccgctttt
     1261 ggtctggtga tgtgcgccct gctgatagtg agtttttata cttcaatcag tggaatactg
     1321 aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc atatgcgttc
     1381 gccaatgcta tatttggtgg gtcggcggag tacgtagcgt tgtcgctgaa atcaatagga
     1441 atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt tctggtttca
     1501 ctaacattgc accgtaaagg aaaagggatg cggctttagt gacgggtcag ttgccagacg
     1561 gtatagccgg tgcttgcacc ggcgatatcc caggcaaaat ccttccagct ccagccgctc
     1621 ccctcagggc ggctatccca aagctctttt gacgccccca gactgacaga gaacatcaat
     1681 ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc atttccggcg
     1741 gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct ccagttatcg
     1801 ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag aatacgcaaa
     1861 acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag gatgcggcta
     1921 attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac cttcaccgga
     1981 taatcggcaa tactttgcag atcgcggtag tgcttaacga tggtggtatg ctcgcggatc
     2041 agatccagtt ctttctctcg ctgtggcgca agctccagtt gcggatcgtg gatcaaaatg
     2101 gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt gatcaaaatc
     2161 cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc tttccataac
     2221 cgaactacta actggtcagt gttgacgtaa tactgtaaac gactcaggaa acgacgcaga
     2281 ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc ttccggaatg
     2341 tagaagtcat tcgcggtttt atcaccaaca ataatttcga cctttttccc ttcgcgcagc
     2401 aactggataa tattgcgcac aaggattgct ggtaggttga agtaaggtgt gcagatggtt
     2461 agtttctgct cggcacaagg cataagatgg aaaatagtct tgttcaacag actcgatttc
     2521 cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc ctggaaatga
     2581 taagcggcat cacgcagctc ctggcggaac aggcgaatgt cgttcttgat ttccgggctt
     2641 tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat attctgtgta
     2701 acccattcaa acataatgtc tgacatctta cggttacgga tcagatgata acggtcgtag
     2761 cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct ataaagtacg
     2821 ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg ggtattgatt
     2881 ggaacgccat aaaccggaac atttacgccc ggattttcct gcgccatgcg gcagtaccag
     2941 tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg atgccagtcg
     3001 accagcaccc gcacatccag ttccggacgc tgccttttag cctcatacaa cgcgttcaga
     3061 atgcctttgc caccgtcatc ctgttcgaga tacagggcaa caatgcaaat gcgctgcttc
     3121 gcgctggcta ttttttccag caacgtctcc cggaagtcgg cgggagcgta aaagaaatcg
     3181 acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg atgtttatta
     3241 cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag ggtcctctgt
     3301 gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt ggtcaacatt
     3361 tccagtacct tactcatgat tcctcgcgct gggcaagact tagcgtaagc ccaacgatcc
     3421 cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggccagcgcc accatgccac
     3481 gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc gtataggtaa
     3541 tcaacttttc cattaagcgt cgacctaagc ctaacccttt aagatccgag cgaaccagta
     3601 cggcaaattc ggcatcgatg ttatcagggt cggagatcgc acgtgtgacg ccgaggatct
     3661 cttccgtttg atcaattcgt cgtaccgcta caaatgccat ttcccgatcg tagtcgatct
     3721 gcgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag tagcggtaat
     3781 aaagatcttc tttggtgact cgcgaaatga attgctgaag ctgtggctca tcttctggca
     3841 aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct tccagctgat
     3901 gcggataagg gcgcacagcc agtcggctct cgttatcacc ttcaaacggc gcgatatcca
     3961 gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga atatccagac
     4021 gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc tggctcaagc
     4081 ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt ttgatcccct
     4141 gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg acggcttgat
     4201 cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg aaaaccggat
     4261 cgtgctcaac cacaacccgc aactcctgag cgccagcacg gttagccata ctttgcacca
     4321 acaggccgtg gacccgcgcc tgcggccagg ccatttttac gcgatcgaaa atagcgtttg
     4381 ccgcttgctg gacttcattg gctgtacgta ggtagagcat gacgccctga acttccgatt
     4441 tatgtggaat atccggcgag cgcaatttca gtgccaccgg atagccaatc tgttcggcaa
     4501 tatgtaccgc ttcggtgcta tcactggcaa tccaggtagg gagagtattc ataccatacg
     4561 cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg gcaatcgcct
     4621 gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc aacgccggcg
     4681 tttcgcgtag ttgcttctga ttacgccggt actcgaccat atgcataaaa gcagtgatgg
     4741 ttccttccgg agtacggtag gtcggcagcc cggcttcgct gaataaacgt cgtgcctctt
     4801 gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg cggggatgat
     4861 gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg gcgctgggcg
     4921 aatgaataac catcagcgca tcaaaatcct ggctgtggag cagaatgtcc agcgttttga
     4981 cataatgctc gctgctggcg tcatcgcgca gatcgagcgg gttagagatt gccacatgcc
     5041 ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc agcttgccat
     5101 tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc agcaccgttg ctgataatca
     5161 tcaaccggtc gccacgcagc gggcgcatat ggctaagggt ttccaccgcc gaaaacagtt
     5221 cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agctgcatcc cacgccggat
     5281 ccattcctgc cgtggtgttc aacagacgtt gagccgccgg gctacgtccg cttttaatca
     5341 ccaaaatcgg tttattgcgc gaggcactac gggctgccga aacaaagcgt cgcgcgtcgc
     5401 ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgcgcc agatagtcga
     5461 gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag gaaaagccca
     5521 tctcacgctg ttgcgcccag tcgaggatgg tgttggagac agcagccgat tgcgaaataa
     5581 acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga ccttgccagg
     5641 gagccagtaa gcccagactg tttggtccta gcaggcgcat gttatggcgc agggcgcagg
     5701 cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt ttacagcctt
     5761 tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat aaaaccgcaa
     5821 ggtcgggtgt aaagggcaag ttggcaatat ccggccaggc caacacaccc aacaccgctt
     5881 tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc aggttacgca
     5941 tcatcaggta acctgcgcga ttgggtttca tcgatgcgcc aattaccgca atcgattttg
     6001 gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga agtgaccgaa
     6061 tgattttaaa cgctttctaa ctgttctgct gtgatgcttc ccggatgttg cgtttttcct
     6121 gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgtcgc cgccctggta
     6181 tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg gccttcagcc
     6241 tgggcttcac gcaggcgata ggcagaaagc cgagaaagat cgacggaaat gacgggaaga
     6301 ttatccagat acggactttt acgaaacatc ttgcgagctt ctggccaggt accatcgagc
     6361 atgataaata gcggaggctt atcggcaggt ggtgtgaaga tcacttcccg ttgctcatca
     6421 gcatacgaag cgggaaagac caccattggc tgatagtctg ggttttgcac cagatccagc
     6481 aaatcttgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg caaaatatca
     6541 gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat cagcaaacag
     6601 aagtgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt ctctggcaaa
     6661 agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg tgttgcgcgc
     6721 gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg tcataagaga gcgtcgaaaa
     6781 aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa gattaaagag
     6841 attcgttcag ccagctgtcg aacggcgctt tcggtactgc gccattaagc atgtcgacaa
     6901 cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag cgactgctca
     6961 attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta cgctcttgcg
     7021 cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt gcccagaagt
     7081 cgatcaccac aggtagatca tctttcagca atttgtcgag cgtttcaccg gtcgcattaa
     7141 tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca tcttcgatcc
     7201 gatcgtcggg aatgcgattg atggcctgac aatgggtaca aacggtattc ataactaacc
     7261 tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat atgttacata
     7321 ttatcgggga cattgttcca gacaacaatc tgttttattc aatgggtatg atagacacag
     7381 gctttggaac gaaatcatgg cgaaccgcgc gcacttcggg taatctgcgc gcttcgcgca
     7441 gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa ggtcaaagtg
     7501 atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg taccgggaaa
     7561 gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc ccgcgatgac
     7621 aaacagagtc agcaccgtga ccgcaagtgg gaagattcgc cgtggcgcac ggtttcccgc
     7681 gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg taaaagtttt
     7741 attgatccgg aagttttgcg tcgtcagcgt gcggaagaaa cccgcgtcta cggcgaaaac
     7801 gcctgtcagg cactgttcca gagccgtccg gaagcgattg ttcgcgcctg gtttatccag
     7861 agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg caaagcgtac
     7921 catgtggtgg atgaagcgga actgacaaaa gcgtcaggca cggaacatca cggcggcgtt
     7981 tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag ccaggcaggc
     8041 gcgcaggatt gtgtactggc cctggaaaac gaatctaacc cgcataacct gggtggcatg
     8101 atgcgtagct gtgcgcactt cggcgtgaaa ggtgttgtgg tgcaggatgc agcactgctg
     8161 gagtctgggg cggctatccg taccgcagaa ggcggcgcag agcacgttca gccgattact
     8221 ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg cgggttacac cgtagtgaca
     8281 acttccagcg agcagggtaa accgctgttc aaaaccagtc tgccagcgaa aatggtactg
     8341 gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa cgatctgcgc
     8401 gtgaagattg atggtactgg caacgttgcc gggctgaata tctctgtcgc aaccggcgtt
     8461 cttcttggcg aatggtggcg tcagaataaa gcctgataaa tcagccgggt ggcaactctg
     8521 ccatccggca ttttcccgca aatttactca ctctctgccg gtaatactgg catccagtca
     8581 atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg gttgcagcca
     8641 aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac atgatggcgt
     8701 tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa aaacaccacg
     8761 ccttcgcgat gctgatttat gaggctgatg actttatcag taaacgtctc ccagccgagg
     8821 ctggcgtggg aatgcgcctg accagcgcga accgtcaaga cggtattgag cagcagaaca
     8881 ccctgacgcg cccagctttc aagataacca tgattagggc gggtgaaacc gggaatagta
     8941 ttttccagct ctttatacat attcagcagt gacggtggtg tcgcaatgcc ggggcgaacg
     9001 gaaaatgcca gaccatgcgc ctgtcctggt ccgtgataag gatcctgacc gagaatcacc
     9061 actttaacgt cacccaactc cgtaaagcgg aacgcgttaa agacatcttt ttgtggtgga
     9121 tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt aagaaaatag
     9181 ggttgctgct tctcttcagc cagcacatca tgccaggtta attcgttagc catctcgctc
     9241 tcctgcgaat ctttaatccg cctagcttaa ctgcttcttt ctacagaaca aaatcatgct
     9301 cattttatag acaaaaagac atgcaaaaaa atgtaaattg gtaaaatttc acatctgcat
     9361 aagttgatgt aaaacaataa aatccatttg t
//
LOCUS       DACADF010000164         9336 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.166, whole genome shotgun sequence.
ACCESSION   DACADF010000164 DACADF010000000
VERSION     DACADF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.166"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..314
                     /locus_tag="ISB30_004041"
     CDS             <1..314
                     /locus_tag="ISB30_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP1991620.1"
                     /translation="DQGSHYTSRQFRQLLWRYRIRQSMSRRGNCWDNSPMERFFRSLK
                     NEWVPATGYVSFSDAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNAEASF
                     S"
     gene            397..654
                     /locus_tag="ISB30_004042"
     CDS             397..654
                     /locus_tag="ISB30_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991621.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1211..1978)
                     /gene="fecE"
                     /locus_tag="ISB30_004043"
     CDS             complement(1211..1978)
                     /gene="fecE"
                     /locus_tag="ISB30_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HAP1991622.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1979..2935)
                     /gene="fecD"
                     /locus_tag="ISB30_004044"
     CDS             complement(1979..2935)
                     /gene="fecD"
                     /locus_tag="ISB30_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HAP1991623.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2932..3930)
                     /gene="fecC"
                     /locus_tag="ISB30_004045"
     CDS             complement(2932..3930)
                     /gene="fecC"
                     /locus_tag="ISB30_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HAP1991624.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3927..4829)
                     /gene="fecB"
                     /locus_tag="ISB30_004046"
     CDS             complement(3927..4829)
                     /gene="fecB"
                     /locus_tag="ISB30_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HAP1991625.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4874..7198)
                     /gene="fecA"
                     /locus_tag="ISB30_004047"
     CDS             complement(4874..7198)
                     /gene="fecA"
                     /locus_tag="ISB30_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HAP1991626.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLTSRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7285..8238)
                     /gene="fecR"
                     /locus_tag="ISB30_004048"
     CDS             complement(7285..8238)
                     /gene="fecR"
                     /locus_tag="ISB30_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HAP1991627.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRILTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8235..8756)
                     /locus_tag="ISB30_004049"
     CDS             complement(8235..8756)
                     /locus_tag="ISB30_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP1991628.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9048..9320
                     /locus_tag="ISB30_004050"
                     /pseudo
     CDS             9048..9320
                     /locus_tag="ISB30_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2063 a         2581 c         2651 g         2041 t
ORIGIN      
        1 gcgatcaagg cagtcattat acgagcaggc agttccggca gttactgtgg cgataccgga
       61 tcaggcagag tatgagtcgg cgtggaaact gctgggataa cagcccaatg gagcgcttct
      121 tcaggagtct gaagaacgaa tgggtgccag cgacgggcta tgtaagcttc agcgatgcag
      181 ctcacgcaat aacggactat atcgttggat attacagcgc actaagaccg cacgaatata
      241 atggtgggtt accaccaaac gaatcagaaa accgatactg gaaaaactct aacgcggagg
      301 ccagttttag ttgaccacaa cagactacct gaagggagcc gcggtcgcct ggcagttgca
      361 gtagcaggag atcatccagc cgcagtacag gtcacgatga ctctggttaa tgataccggc
      421 tttgaccccg tattttccgg ctctatcgct gaatcatggc gtcagcagcc gtgcacacca
      481 tcctattgtt gtgactggga ggctgccacc atgcttcgcg ctttccctct ggcgaaaaag
      541 ggagaaggac gggcccgtct gccttcactt tatgccagct tcggtaagct gggtgagaca
      601 ccgactcatg aagatatcat tgataacaat cgatccatca actggcctgt ataacgtggc
      661 tgccggtgat taagaaagct gcacctacct aagtagtagc aaacgcacac tttttagaaa
      721 aatcgatggt cagaaactgg attagcaatt ccgttccagg gttgcttttg atttacgttg
      781 gcgtctgatc attgatttat cctcaaaagc ccaacctcat tggtaatgaa ccagctccgt
      841 gaatgtccgc tctggcacag agcgaaattt tttgatctcc cccctgaaat ctaaacttag
      901 tcatgtcacg tttttgggtt tctaaaattt taacttcgcg tttttcgttg ccgtaagggt
      961 tatacagaaa tgtccgttaa gcagagttca aaattgattg ccgtgatcac gactggtttg
     1021 aaagccgcgc ccaagcctgt acagctctgg tttgcgttga ttatgaacct gtcagcctaa
     1081 agcaagcgga tggacgatga gtattggtaa tctttcagag tccggaaaag ttcagcccca
     1141 gtctgaacag gcttgctggc gccagtccag tttcattcag tcgtggtttg gttcttacgg
     1201 cctgtgcaat ctacctcatt aggcacatcg gcctgccaga taccggctcg gggtgtattt
     1261 ccgcttccac gctgaatact gttctcagca atcctggggt catcacctct tctggtgtgc
     1321 cttgcgccat aacatgtccg tttgccatta ccaccagttg atcgcagtac cggctagcct
     1381 gattaaggtc gtgcagcaca gcgaccaccg ttttcccctg agtccggagt tcgcccatca
     1441 accgcatcag gtccacctgg tgattgatat caagataggt ggttggctca tcaagtaata
     1501 caacgggcgt attctgggcc aggaccattg ccagaaatgc gcgctggcgc tgaccgccgg
     1561 aaagctcggt taaccgacga acggcaagat gattgatccg ggtctggttc atggcgacat
     1621 taactcgtgc attgtcttca gcggagagac gcccccagag tgacagccag ggattacgac
     1681 catacgaaac cagctcctgg actgtgatcc cctctggcgt taaatggtgc tgaggcagca
     1741 gcgaaagcct gcgggccaac tggcgcgatg agagcatatt tatgggatta tcgccgagaa
     1801 atacggtgcc agactgcggc attaaaagcc gcgaaaaaca gtttaacagc gtcgatttcc
     1861 cgcaaccgtt aggaccgatc agggcggtga tcttccccgt tggcagtgag agtgaaacgt
     1921 cgttaagtac cttgtctgtc ccgtaactga ccgtcagatt ttcagttcgt aaagtcattt
     1981 atcgcattct cacaagcaac cagacaaacc acggcgcacc gataatggcg gtcagcacgc
     2041 caaccgggag ctccagtggg ggatgaataa ttctcgccag cagatcggca accaccaaca
     2101 gcaacgcacc tgtcagggcc gaaacaggca gcagtctgcg gtgacgtcca ccggtgatgc
     2161 tacgcatcat atgcggcacc acgagaccaa taaagctaat cgggccgcag gcggccacgc
     2221 cggtagatgt catggcgaca gctagtaaca aagcccagaa tcgggtatgg ggcaccgaca
     2281 caccgagcgt ggtggcgcgc gcatcgccga gtgcaaggag gtcgagatcg cggcaaaaac
     2341 tcaggctcag cggcagaaat aaaatcatca gcgggatggc aatcttcaca aagctccagt
     2401 cacggcccca taagctgccg gtcagccaca gcagggcgtt gttcacatcc tgcgggcgcg
     2461 agagcatcag ataatccgtc aggctggccc agcatgcaga aagcgccacg ccggtgagcg
     2521 ccagcttcat cggctggtgg gtctttgcca gcatcttcag caatatcaac cccgccatgc
     2581 cgcccgcaaa ggccagcagc ggcagcacca tcacgggcag tgacggcata agaagtagag
     2641 cccccacaga ggccaggctg gcggcatggt taacaccgag aatatccggt gatgccagag
     2701 ggttgcgcac aatcccctgt atcagcacgc ccgccacggc gagggctgca ccgacaaaca
     2761 gtgccagcag caagcgcggc agtcggtact ccatcaatac ataataatgc tcgcgtccgg
     2821 cctgccagtc ggtcagcagc gcgcgccacg gcacggggat cactcccata tggagtgata
     2881 acagcgcaca gcccgccagg gcaagggtga tgaaaataac cagcgcaatt ttcatcctcg
     2941 cctcctcaca agccagacaa agcaagggct gccaatcagc gccagcactg cgcctgcggg
     3001 cagatctccg gggaaggcca gtgcgcgtgc gagtacatct gccagcagca tcagcgtggc
     3061 ccccagcagc atgctcactg gcagtacgtt gcgctgatcg aagcctgccc agaagcgcgc
     3121 cagatgtggc accagcagac cgataaacgc caccggacct gccacgctga cgcacgcacc
     3181 aaccagaagc agcactaaca tattgatgac caaacgtagc ctcgtcaggt tcactcccag
     3241 cgtatgggcg gtgctgtcgc tgaggttgag caggttcagt tgattcgcca gcagcaacac
     3301 gacagggact gcagtgacca ccaccggcaa gagctgccag acatcctgcc agcgggcgtg
     3361 ggacactccg cctgccagcc agtaaaagat gccgtaagca tgatcttcgg ccagcagcag
     3421 ggtgatgcgg gtaaggccca tacaaaaggc cgacagcgcg atacccgcga ggatcagttt
     3481 gtttctgtca tgggtatgac gaaatccgcc tcctgcggtc atgaccagca gccagctcac
     3541 gccgcccccg catgccgcga tgaacgacag agaatagcct gcaatcggcg tcggactcag
     3601 cgcgctggta agcgccatag ccagcgccgc gccgctgtta atgccgagca gtgaaggaga
     3661 ggccattggg ttgtgggtca gggtttgcag cagcgtgccc gcgagcgcca ggcttgcgcc
     3721 gatcagaacg gcgaccaggc ttcgtggcaa acgaaggttt tgcaccagcg cttctggtag
     3781 cgttggcgtg tgtccaggca gcagggcgcg ggttgcatct gctccggaaa caggaatggc
     3841 cgagtagcaa aacagactca gccagaaaat aataataagt gctgcaacgg gaagccccca
     3901 cagcagcacc gggtgtttta tcgcggtcat ttcacaacgg taagcggctg atggtggaag
     3961 atttttaccg tgtcagcggc aatacgctct gcagcaaaaa taccgcgcat ccgcgcccag
     4021 gtgttactgt cgaccgaagc aacctgctgc ttctgcgcgg cggttaacat ctgccagagc
     4081 ggatcttgtt gccagcgttt aacaatgctc tcttcgcgat agtgggcaac cagcagccag
     4141 gcaggattga ccgccagcag ttgctccagg ccgatggacg gcatggacgc acccgccatc
     4201 gcagcgggaa cgttcagccc cagagaggcc agcacgctgc cggtccaggt ctcctgagta
     4261 tgcaggttga attgctgttc gcgtgatgtg ccaaaggcca cgcgtgtccc tttgggaagc
     4321 tggctggccc actgcgccat cctctcttta tgttgttcca ggcgtgcctg catctctcgc
     4381 tttttaccca ccatttcgcc gatgatagcc gcagattgca aattttcagc gtaggtttcg
     4441 ttgcgggact taagcagcag taccggcgcg atttgctgca aggcgatgta aacccccgca
     4501 tggcgactgc tgtcggcaat gatcaggtct ggtttcagag cggcaatggc ttccaggctc
     4561 ggctgcgcgc gcgttccgac ggactgccac ggtttcaggt gcgcacgcac ttcgggcagg
     4621 atgcgttttg catcgttatc gtcggcaata ccgatcgggc tgacgtccac ggcggccagc
     4681 gcatcggcga acgagagttc cagcaccaca atccgttgtg gcgttttttc gagtgtaaac
     4741 gtgccgtgtt cgtcctgaac cgtggcggca aaggcgtggc tgatcaccag cagcaggcct
     4801 gcaaaaacaa aacggataaa tgccaacata atcacattcc agctaaaagc ccggcaagcc
     4861 gggcgttaac acatcagaac ttcaacgacc cctgcatata cagcgtgcgc ggctggcctg
     4921 catagatgcc tttgttgttg tcgtcataag agcggatgaa gtagtcctgg tcgaagatgt
     4981 ttttcacacc gaatgccagg ttcagatctg ccatctgcgg gccaaagtca tacgccacgc
     5041 gtgcgcccca gagcatgaag ccgggaatgc ggccggtact gccgtcggcg ctctctttca
     5101 ccgtattggc gttatccgca aactggctgg actggaaatc gctgttcaga ttgaacgtcc
     5161 agtttcctgg cttgtagtcc acgcccagcg tgcctttatg tttcggggag aatggtacca
     5221 gattgccgta ggtgtcgcct ttctcgcgga tttccgcgtt cacatacgca tagctggcgt
     5281 agatggaaac gttatcaagc gttggcgtta gcgtacccag atcgtaacgt gcctgcgttt
     5341 ccagcccggt atggcgcgtt ttgccacgtg cagtgacggt gtcgttggtc tggttggagt
     5401 cgtactgatt gttaaagtta atcaggaaca gccccatttc cgccgtcagc gcgccgtcgt
     5461 cgtagcgggt accgagttcc caggttcgcg ctttttccgg ttccacattg ccgctttgca
     5521 cagccttgcc aatctggctg tactgtacgg tgccgaacga gccttcagtg tttgcataaa
     5581 gattccagct gtcagtcagg tgatagagca cgttcaacgc cggaagcggt gcgttatagc
     5641 tcacttcttc gtgcgtgcct gtgatggcgt tgttctggta tgactcgata tgttcgaaac
     5701 gcatacccgg cgtgatggtc cagttgccga tgtcgatttt gtcatccaga taccaggcgt
     5761 gcgcctcggt gccggaacgc gtatcgcggt cgtaagggct tgagccggac ggcaactgcc
     5821 cgctgctggt ggcggtgtag taacgcattt catgcgttga ttcattcaca tagcgatagc
     5881 ccacgcccac ttcgtgcgcg gaagggccga tcataaagat ctggctgtag cgtggctcaa
     5941 taccgcgcac ccagtagtta cgcggcgaga gggtgatgcg tttgccttgc tccaggtagc
     6001 cgctgcgcag ggtttgggtg tagaacccct gaatgttgaa tttatgctgg ctgtctggct
     6061 ggaactggta gcccaggctc gccagcttgc gacgacccca gaagcggtca tacgggcggg
     6121 tggattgcca gcgatcggcg tcgtaatccg cgcgagacag gccaccgggc atgtcggctt
     6181 caccgtcgta atattgcagc aggctgttga aggtgtgcac ctcatccggc gcatatttgc
     6241 ttttcagcat caggtcgtcg atgcgggtgg cgctgtgctc gcgccagtca ctgccgcgcg
     6301 tgccggagta gagcagcgcg gtgccaaaac cgttgtccgc tgtgccgccc accatcaggt
     6361 tgtgcgtctc tttcgggttg ttttgtgaag aggttgggct gagctgacct tccacgcccg
     6421 cctcgatacc aaagtcctgc ggaatggcac gggtaacaaa gttcaccacg ccgcccacgc
     6481 tctgcggtcc gtaacgcacc gcaccaccac cgcgtaccac gtcaatggca tccatgttgc
     6541 cgagcgaaac gggagccagt gaaagctgcg gctgaccgta aggggcgaag gggacgggga
     6601 tgccgtccat caggacggtc gagcggctgg tgaggcgcgg gttcaggccc cggatgccaa
     6661 agttcatcgc caggtcgtgg ctgccggtgc cgttgttttc cggcgcgctg acgccaggga
     6721 tgcggttaag tacctcacgc atggtggttg cgccggtttt ggcgaaatcc tcacggcgga
     6781 tcacgtcacg cgcgccagca tgttcaaata cgtcgttttc acgcgcatca cccagccagt
     6841 cgccgaccac ggtcagggca tcttcttttg gtgcgggcgc gggctccagc gtccagctat
     6901 tatttcccag cggttttacc tgcagtccgc tgccgtccag cagttgttgc aggccgctct
     6961 cgacgtcgta atcgccgtgc aggccgttgc tctgcttgcc gcgcgtcagg ctggcgtcaa
     7021 ccgagagggt aaatccgctg tgtgcggcat actgattgag cgctttatcg agcgatcccg
     7081 gtgcgatatt aacctgtgca gcaaaagcgg aaaacgagag accggccagc ggcagcaggc
     7141 tcaggcgaat ggcgttaacc aaaggtgttg ttttacgaaa aacgcgtaac ggcgtcatac
     7201 cttccccatc atcatttttg ttgtgttcag ctatgagtcg aacgagaagg gtaaaaagga
     7261 caatcgaaat aagaattatt ttccttacag tggtgaaatg tttatccagt accgcgtaat
     7321 agactgaatt ttaacgggaa gcgtttgcgc gataacgttc aggatcgcat cggtattttt
     7381 cagcgggaac gtcccgctca ggcgcagccc ggcaacggcg ggatcgcagc gcagcacgcc
     7441 gttgcggtaa cgggttagcg tggctatcac ctcacccagt ggtttatcgc tgaagctcag
     7501 gatgtccttc gtccagcttg tactctcgtc atccagcggt ttcactgcgc caaactcaga
     7561 ggcgctgaac tgcaggcttt caccagcgtt cacgatgcgt ttttgcgcgg gggcactggc
     7621 gagaagcact tccacagcgt gctgctgaac gtcaagctgc gtgaaattat cctgctggcg
     7681 gacggtaaat tctgtcccta aagcagtgag ctggccctga cgggtcagga tacggaaggg
     7741 gcgttgcagg gcatctttcg cggtagtaat ggcgatttca ccgtaccaga gccggacggt
     7801 gcgctgatgc gcatcaaaac gcacatccgc cgcgctttgg gtattcagcg tgagcaggga
     7861 gccatcttcc agttgctgac ggctgactgt gcctttggcg gtgcggtaat ctgcccgcag
     7921 accttcgccg gtttccgact gccagagctg ccagcctccg ccagcgccga gcaacagcag
     7981 taatcctttc atcacgtgac ggcgggtgag gcgggtatcg tgcaacgccc ggctggcaac
     8041 gtcgccaggc acaccgccaa gctggttgcg caggttttca acctgctgcc aggcccactg
     8101 gttatcctga tcctgttcat accactgttg ccagcgcgct tcctgttgtg ggctgacgcg
     8161 ctcgccgctt agcacggcat accagtggga agctgaacgc agcgcctgac ggcgggaatc
     8221 ggttaacaaa ggattcataa cccatactcc agacggaaca gcaggcagtg ctcgacggct
     8281 ttcgccacgt attttttcac ggagctgatg gaaacaccga gtttgtgcgc aatctcgctg
     8341 tatgtcagac catccagttg cgaaagcaga aacgcttcac gtgttttgcc gtttagcccg
     8401 tccagcatgc tgtcgaggag ttgtagggtc tcgagttggc tttcgcgttc ctcaggtgaa
     8461 ggcgctcccc cctccggcat aagcgccagc atctccagat acgctttttc cagcgcgttt
     8521 cggcgaaaca ggtccaccat cacgcgtttg gcgatagtgc agaggaagga gcgaggatcg
     8581 cggatcgtcg agagcgtttc gctgaccatt acccgcaaaa aagtgtcctg ggcaatgtca
     8641 tctgcatcaa aagcagactg gagtttgcgc gtcagccagc ttttcaacca gccgtgatgt
     8701 gtgccataaa gcgactcgaa cgttaaggaa gctgtggtag tggcgcggtc agacatgcgg
     8761 agtgcatcaa aagttaatta tcacgtagtc atattaatat gagaatggtt atcattacaa
     8821 ttggaaataa aattgtttcc aatagacatt tttaacatgt tgtttttcta agtgttataa
     8881 ggtaggtata aaatgggatg gagcctctgc ttctggcatg tgtcggtcag aatgactcat
     8941 gatgtggtct gctattattg acatcctcac tgccctaaag gatggggatt tcggtaatgc
     9001 tgccaactta ctgatttagt gtatgatggt gattttaagg tgcttgcgtg gcttccattt
     9061 ccatcagatg tccttcctgc tccgctactg aaggcgtggt gcgtaacggc aaaagcactg
     9121 ccggacatca gcgctatctc tgctctcatt gccgtaaaac atggcaacta cagttcactt
     9181 acaccgcctc tcagcccggt acgcaccaga aaatcattga tatggccatg aatggcgtcg
     9241 gatgtcgcgc cagtgcacgc attatgggcg ttggcctcaa cacggtttta cgtcacttaa
     9301 aaaactcagg ccgtagtcgg taacctcgcg cataca
//
LOCUS       DACADF010000165         9325 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.167, whole genome shotgun sequence.
ACCESSION   DACADF010000165 DACADF010000000
VERSION     DACADF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.167"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..1143
                     /locus_tag="ISB30_004051"
     CDS             <1..1143
                     /locus_tag="ISB30_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP1991629.1"
                     /translation="ESNFTHGHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHT
                     SFSVANAPDEPHIEQLVGECRVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIH
                     KLSGRRNKPFIAINCAAIPEQLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLD
                     EIGDMPLMLQAKLLRAIEAREILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDL
                     FYRLNVIPITLPPLRERQEDIELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPG
                     NLRELSNLMEYLVNVVPSGEVIDSTLLPPNLLNNGTTEQSDVTEASEAHLSLDDAGGT
                     ALEEMEKQMIREALSRHNSKKEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1182..1661)
                     /gene="xdhC"
                     /locus_tag="ISB30_004052"
     CDS             complement(1182..1661)
                     /gene="xdhC"
                     /locus_tag="ISB30_004052"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HAP1991630.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(1658..2536)
                     /gene="xdhB"
                     /locus_tag="ISB30_004053"
     CDS             complement(1658..2536)
                     /gene="xdhB"
                     /locus_tag="ISB30_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAP1991631.1"
                     /translation="MFDFASYHRAATLADAINLLTDNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGALRIGSATTFTQLIEDPVTQRHLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(2547..4844)
                     /gene="xdhA"
                     /locus_tag="ISB30_004054"
     CDS             complement(2547..4844)
                     /gene="xdhA"
                     /locus_tag="ISB30_004054"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HAP1991632.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAVQLVSIEWQELPVITSSEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5258..6013
                     /locus_tag="ISB30_004055"
     CDS             5258..6013
                     /locus_tag="ISB30_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP1991633.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6092..6165
                     /locus_tag="ISB30_004056"
     tRNA            6092..6165
                     /locus_tag="ISB30_004056"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6124..6126,aa:Gly,seq:ccc)
     gene            complement(6482..7711)
                     /locus_tag="ISB30_004057"
     CDS             complement(6482..7711)
                     /locus_tag="ISB30_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAP1991634.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            7966..9147
                     /locus_tag="ISB30_004058"
     CDS             7966..9147
                     /locus_tag="ISB30_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAP1991635.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT         2392 a         2194 c         2353 g         2386 t
ORIGIN      
        1 gagagtaatt ttacccatgg tcatatgcaa catattgttt cgtgggatga taaaagtgaa
       61 ttaatcattg gtcagttgca taacatccag ggccgacaac tatttttaat ggcgtttcac
      121 caatcgcata ccagtttttc tgtagcaaat gcacctgatg aaccgcatat tgaacaattg
      181 gttggcgagt gccgtgttat gcggcaatta aaacgactca ttagccgtat tgcacccagc
      241 ccatccagcg ttatggtggt tggtgaaagc ggcacgggta aagaagtcgt cgcccgtgca
      301 atccataagt tgagcggaag acggaataaa ccctttattg ctatcaactg tgccgcgatt
      361 ccggaacagc ttctggagag cgaactgttc ggttatgtta aaggcgcatt tactggcgct
      421 tctgccaacg gtaaaacagg gttgattcag gcggcgaata cgggcacgct gtttctcgat
      481 gaaattggtg atatgccatt aatgttgcag gctaaattac tgcgcgctat tgaggcgcgt
      541 gaaattctgc cgattggtgc cagtagccca atacaagtcg acatccgcat catttctgca
      601 actaatcaga atttggccca gttcattgcc gaaggtaaat tccgcgaaga tctcttctac
      661 cgacttaatg ttatcccgat aactcttcca ccgctgcgtg aacgtcagga agatattgaa
      721 ttactagtgc attacttttt acatctgcat acccgtcgtc tgggatcggt ttatcctggc
      781 attgctcctg atgtcgtcga aatattgcgt aagcatcgtt ggcccggaaa cctgcgcgag
      841 ttaagcaatt tgatggaata tctggttaac gttgttcctt caggtgaagt tatcgacagc
      901 acgctattgc cgccaaatct gctgaataat ggcacaacgg agcaaagtga tgtaacagag
      961 gccagtgagg cgcacctgtc actcgatgat gcgggcggca cggcgctgga agagatggaa
     1021 aagcaaatga tccgcgaggc gctttcacgt cataacagca agaaggaagt tgctgatgaa
     1081 ctgggcatcg gcattgctac gctctatcgc aagattaaga aatatgagtt gttaaacaca
     1141 taacctctga gaatccggcc tgaattcagg ccggatatct tttactttgt tttctcgcaa
     1201 tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt tcccgccagt
     1261 ccacgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag cattgccgtg
     1321 gtagccataa tcaggcccgg cgtacaaaac ccgcattgca ctgcaccaga tttcgcataa
     1381 gccagttgga catgagaaag tttaccgcct ttcgcttcac cttccagcgt gcggatctct
     1441 tttccttcag cccaggccgc aaggaataag caactgtcta tcgcagtgcc gtcgaccagc
     1501 accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag cagtccttgt
     1561 tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa aggcatcccg
     1621 ttaatggtgc attcgatggt aattgtttcg ctgtgattca ttgcaatttc ccccccgccg
     1681 cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc agacgaaact
     1741 ctttactggc ccgccatgac gaacgcgggg cgacatcttg caggactgat tcgctgatgg
     1801 cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca tgttggcagc
     1861 gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa ttgccgttat
     1921 ccagtcggca atgtgcggcg cagccaattg ttgaaatatc cattgcgtcg cgcatggcat
     1981 atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga aaggcgacaa
     2041 ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca ttaatcggga
     2101 cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt agcgttggcg
     2161 tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc gccgtaggtg gcgacattac
     2221 ggatctgcgg cccggcgatt gatgcagccg cagcacataa cgccgggaga tgacgttgag
     2281 ttacaggatc ttcaatgagc tgggtaaatg tcgttgcaga gccgatacgt agcgcgccat
     2341 cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca atatggcgat
     2401 aacggtcatt gtgatggtga agctgtatca gtacgtcagt gccaccggcg agcagtttgg
     2461 cctgcgggtt gtcagtcagc aggttgatgg catcggcaag ggttgctgcg cgatggtaag
     2521 aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa ttcttcatat
     2581 aatcgttttg gcgttagcgg cagtgtattg attgcaacac cggtagccat cttcaccgcg
     2641 ttacgaatag cagcggctac aggaattatg ggtggctcac ccagtgactt atgtccgtat
     2701 gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag atccggcatg
     2761 gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt cgcatcgatg
     2821 atcatctctt caaatagcgc ccagccaatg cccatcccca ttccgccgtg tacctgacct
     2881 tctgccagca gcggattaag aatatggccc gaatcatgaa cgttgaggat gcggttgatg
     2941 gttactttac acagtgcaat atcgaccgtc agatcaacaa aggtacagcc aaacgccggt
     3001 gggttagtgg tggttttgat ggagctttcg gcagagagct gcccgccgcg ttcagggtgg
     3061 tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg tcgttcaacc
     3121 agcacgatat ggccttttat cagggtcaga ttcatcgctg actgatgtag catgactgcg
     3181 gcgtgagcga tgattttctc ttttaataac agtgccgcac tgcgcagcgc aggcgcggca
     3241 acatagctct gacgtgaggc aaatgcgccg gggtcgaatg gtgtaacgtc ggtgtcttgt
     3301 gtcgaaatta cgcggacatc gctgaccgga actcccacgg tttctgccac catttgcgag
     3361 aacacggtgt cggcaccctg gccgatttcc gtcgcgccgc tttgcacgtt gatggttcca
     3421 tcctgattca tcaacaggcg cgcgcctgct atttctacgc cgacaggcca ggtgttagag
     3481 gtgtagctaa aacaggcgac gccaacgcca cgacgtaaat tgccttgctg gttctggcac
     3541 tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca ctccggcaac
     3601 cctgcgctgt aaatacgttt tcccgtgagc ggattagcat ctccttcgcg ggcggcgttg
     3661 cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcatcatc aagcatagac
     3721 tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc cgaggggagg
     3781 ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag gtaagcgact
     3841 ttattccccc cagcagaagc aatggagtgc ccgtgagatg cataagcgcc ggtgttagac
     3901 agaacatcca gactataacc tttcaatgtt ccgtcgcggt tcacgcccat ttgcccgtca
     3961 atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct aagagaaact
     4021 ttcaccggaa tgccgccaag tttgcttgtc aggaatgccg ccattggctc ttccagtaca
     4081 tcctgtttat taccaaaacc gccaccgata aacggtttga tgactcgtac gcatgaccag
     4141 ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg ggtgctggaa
     4201 acgatggtaa ttcgcgagtc atcctccatc catgccagcg atgtcacgct ttccatatga
     4261 caatgttgaa taacgggcgt ctgatagtgc ccctgtacct ggtagtcggc ggcatcgatt
     4321 gtttgttgga cattacccgt cgacatcgtg ctttgtttca gtaaattgcc accgttatgg
     4381 attggtgcag cgtcttctgc cagcgccgct tctgacgagg tgataacggg taattcttgc
     4441 cactcaatgc tgaccaattg caccgctttt tctgccgtga gttcatcgcg ggctacgacg
     4501 atggcaacgg cgtcaccatg atgacgaaca tgacgcgtta gcagggcacg atcggcagta
     4561 tcgcgcttgt tttcgtcaag tgtccaggca tgccctgccg tggcgaatgg gatttctggc
     4621 acatcttccc aggtaaaaat cgccaggacg cccggcaaac tcctggcttg ttcatcatta
     4681 atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta acacatgccc
     4741 gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat ggcatcgacg
     4801 cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat cccctcggtt
     4861 tgaacatttg gttcttatgg caaagattta gtcttagcag gcaagaatta cgccagaaac
     4921 gactatatgc gtgaggggag gaaggaagtg tgatgacgtt aaaaaaacac agatatgata
     4981 cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg ataaaatcaa
     5041 tgtgataaat taggattatt acgcatcaag gtgggaaata gggtaaaagt caggaaattc
     5101 gataccacta cagcaaatat tttgctgatg atacttccct ttgccaaatg aaattaacct
     5161 ctttctctat tacatcgttt ttccagaagc aagagattgg gtatcaagag gctggctgct
     5221 atgatatggc gtcttgtttt ttaagcgagg aaagattttg agtgcgggac gcctgaataa
     5281 aaaatctctg ggtatcgtga tgttgttatc ggttggactg cttttggcgg gctgttcggg
     5341 tagcaaatca tccgatacag gaacgtattc cggctccgtt tacaccgtga aacgggggga
     5401 tacgctatat cgtatttcgc gcaccacggg aaccagcgta aaagaactgg cgcgactgaa
     5461 cggcatttcc cccccttaca ccattgaagt aggtcagaaa ctaaaactgg gtggggcgaa
     5521 aagtagcagt agtacacgta aatcaaccgc caaatccacg accaaaaccg catcggttac
     5581 accgtcatca gcggtaccga aatcttcctg gccgccagta gggcaacgtt gttggttatg
     5641 gccaacgaca gggaaagtta tcatgccgta ttcgacagca gatggcggca ataaagggat
     5701 tgatatctca gctccacgag gtacacctat ttacgccgcg ggtgcaggaa aggtggtgta
     5761 tgtgggcaac cagctgcgtg gctacggtaa tctcatcatg attaaacaca gtgaagatta
     5821 cattacggct tacgctcata atgacacgat gctggtaaat aatgggcaaa gcgtgaaggc
     5881 tgggcaaaaa atcgccacta tggggagcac tgatgcggca tctgttcgcc tgcatttcca
     5941 gattcgttac cgtgcaacgg caattgatcc gctacgttac ttgccgcctc agggcagcaa
     6001 gccaaaatgc tgatggcgaa ttaatcagca gtcagcagcg tggtacttgc taaggagagc
     6061 gtaaggttta taatgcctta cgcatctcga agcgggcgta gttcaatggt agaacgagag
     6121 cttcccaagc tctatacgag ggttcgattc ccttcgcccg ctccaattta tcttcacatc
     6181 atctacctgc catttttgtt gtatctgatt ttctatatta tcttaattca agcgcttcag
     6241 acttttcgca atcgccgtaa tacttcccca tttttttgta aataaaaagc gtttacgcaa
     6301 tgcaatcgtc atgtatctta aatagtctta tttaagatgt tcagaactct ttgtgtgttt
     6361 aactggctga taattatata atattacgag agaagtgata gagcggaata ataacaaatg
     6421 aacattatga aaactgggat ggttcaaaat tgaagcaatt ttgaaccatc cacaacggat
     6481 attagctaaa gaacattacc gatacgcaca ggatacccac gatcagggta atcagatttc
     6541 cgatggaacg ccagggttta agcgccggga tgagatacgt tgacagcgta ggcatgatga
     6601 aaagtatcat ggcaatgagc gggccgctga tcgcgtaaat catcgaaatc gcgttcgggt
     6661 taatgcaaca aacaatgaag gtaatcagcg ataccaacat aattgatagt gcgcggttaa
     6721 atgcacgact tttctttaca ccaacctgct gtaatgttgt tttgacgacc tctgtggcac
     6781 cttcaataac gccaaagtac gtacccagga atgatttcga catcgcaact actgcgacaa
     6841 taatcccgga aattgacaac catgctgggg cgttcggcag catagaaagc gccgataaaa
     6901 tggtaacccc ttcttcttta gccgcttcaa tatacgaagg tggaatagaa agcaggcagc
     6961 taaagacaaa gaacaataca cttatgcaga tgatgagata agcgactttc ataatttttt
     7021 tgcatttatc catagcatgt tcgccatatt tttcacgtct gtctatggca aacgtagaaa
     7081 taatgggcgt atggctaaaa gcgaaaacca tcacgggaat cgatatccat atctggtgaa
     7141 gggtattctg attgaactct acctgggttg ttaatagatc aggttgccaa ctaccgacca
     7201 ggtaaataga aagaaataag aaataggcaa tcaatgggaa taccaaaaat cccattaccc
     7261 gaatagtggc atggcgtccc atcagaaaaa tgagattcag gattaataca acccccagac
     7321 tcactagcat acggatgcga agatcaataa ccatatgctt tgccagctgt tccgtaagtg
     7381 agttggtaat tgccactgca tatatcaaca cgacgacaaa aaaggcgatg aagtacagcg
     7441 tggtaatcag attaccaatc ttcttgccat agtagtgtgt taccgcgccc gttatccctt
     7501 cacctgctga tgttttcgat gagaggatga actggcataa ggctttatgt ggccaatatg
     7561 ttaaaggcca ggcgaccaga gcagtaataa acaggaccac agcccccgcc gaacctaact
     7621 gaataggaag gaagagcgtg cctgcaccaa cggctgtgcc gtagagtgcg aaactccaca
     7681 gagtttcttc ttttgaccaa atattagaca ttatctgaac gtatcatcta caaattaaac
     7741 aaaatggagg gcaatttacc ataaaccagg ttaagtatgg gagattaacg ccttatctac
     7801 ctctctattg gtgggttagt ggttgcaaac cttacgtgcc agttatgtct ctgactacta
     7861 ctcaggcgta tttcctttgt gataatggtc acttttttta aacaattaag ataactgtaa
     7921 ttaacaatca tttactattg cactgttaat tggtgcgagg ttgtgatgaa agacgttgtg
     7981 attgtcgggg cgttacggac acctatcggc tgctttcgtg gtgcgttagc gggtcattcc
     8041 gccgtggaac ttggcagcct ggtcgtcaaa gcgttaatag aacgtaccgg cgttcctgca
     8101 tatgcggtgg atgaagtgat tcttggtcag gtgttgactg caggggcagg gcagaatccg
     8161 gcaagacaat cggctattaa aggtggtctt cctaatagcg tttctgcaat cactattaat
     8221 gacgtttgtg gttccgggct taaagcactg catctggcta ctcaggcgat acagtgtggc
     8281 gaggctgata ttgtcatcgc cggtggccag gaaaacatga gccgcgcacc acatgttctg
     8341 actgatagcc gcaccggcgc acagcttggc aatagccagt tggttgatag tcttgtgcat
     8401 gatgggttgt gggatgcctt caatgattat catattggtg tcaccgccga aaatctggct
     8461 cgcgaatatg gcatcagccg tcagttgcag gatgcttacg cacttagctc gcaacaaaaa
     8521 gcgcgagcgg cgattgacgc cggacgattt aaagatgaga tcgtcccggt aataacccaa
     8581 agtaacggtc agacgttggt tgttgatacc gatgaacagc cacgcactga caccagcgca
     8641 gaaggcttag cccgtttaaa tccttcattt gatagtctcg gttctgtgac agcgggtaat
     8701 gcatcatcca taaacgatgg ggcagctgca gtaatgatga tgagcgaagc caaagcacga
     8761 gcgttgaatt tacccgtgct ggcccgcatc cgcgcatttg ccagcgttgg tgtggatccg
     8821 gcattgatgg gaattgcgcc ggtgtatgcg acccgccgtt gcctggagcg tgttggctgg
     8881 cagttggctg atgtcgatct tatcgaggct aatgaagcgt ttgctgcaca ggcgctttcg
     8941 gttggcaaga tgcttgaatg ggatgagcgt cgggtcaatg ttaatggtgg tgcgatcgca
     9001 ctcggtcacc cgataggcgc ttccggttgc cgaatcctgg tttctcttgt tcatgaaatg
     9061 gtgaaacgta atgcccgcaa aggactggca acgctttgta tcggcggagg ccagggtgtg
     9121 gcattgacca ttgaacgtga cgagtagctc tgcattacct ccttaccgat aaatatctgc
     9181 cgtcattaat ctgttgaata atgatggctg gtgagtcgac ataccttttc agggctccct
     9241 gatttttcca ttctcatgac atattcgtga tcgacactgc acttttttta atccttaact
     9301 tgaaaatgaa acattgtttt atttt
//
LOCUS       DACADF010000166         9310 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.168, whole genome shotgun sequence.
ACCESSION   DACADF010000166 DACADF010000000
VERSION     DACADF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.168"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            73..1137
                     /gene="ulaG"
                     /locus_tag="ISB30_004059"
     CDS             73..1137
                     /gene="ulaG"
                     /locus_tag="ISB30_004059"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP1991636.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGANVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1245..2000
                     /gene="ulaR"
                     /locus_tag="ISB30_004060"
     CDS             1245..2000
                     /gene="ulaR"
                     /locus_tag="ISB30_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAP1991637.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(1997..2746)
                     /gene="yjfP"
                     /locus_tag="ISB30_004061"
     CDS             complement(1997..2746)
                     /gene="yjfP"
                     /locus_tag="ISB30_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP1991638.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLTELGNRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2928..3257
                     /gene="bsmA"
                     /locus_tag="ISB30_004062"
     CDS             2928..3257
                     /gene="bsmA"
                     /locus_tag="ISB30_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAP1991639.1"
                     /translation="MVSRKRNSIIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3406..3681
                     /gene="yjfN"
                     /locus_tag="ISB30_004063"
     CDS             3406..3681
                     /gene="yjfN"
                     /locus_tag="ISB30_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAP1991640.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3798..5423)
                     /locus_tag="ISB30_004064"
     CDS             complement(3798..5423)
                     /locus_tag="ISB30_004064"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAP1991641.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLDDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     TWCKVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5507..6670)
                     /locus_tag="ISB30_004065"
     CDS             complement(5507..6670)
                     /locus_tag="ISB30_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAP1991642.1"
                     /translation="MLRHNVPVRRDLDRIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEELGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6673..7311)
                     /locus_tag="ISB30_004066"
     CDS             complement(6673..7311)
                     /locus_tag="ISB30_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAP1991643.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7321..7719)
                     /locus_tag="ISB30_004067"
     CDS             complement(7321..7719)
                     /locus_tag="ISB30_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAP1991644.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7737..8396)
                     /locus_tag="ISB30_004068"
     CDS             complement(7737..8396)
                     /locus_tag="ISB30_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HAP1991645.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLSFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFSMGYQRQVTGDTYEYLLLNGEESFNDLGEPEWLFSRALGIDIPLTSLHII
                     G"
     gene            complement(8446..9144)
                     /locus_tag="ISB30_004069"
     CDS             complement(8446..9144)
                     /locus_tag="ISB30_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HAP1991646.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9163..>9310)
                     /locus_tag="ISB30_004070"
     CDS             complement(9163..>9310)
                     /locus_tag="ISB30_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HAP1991647.1"
                     /translation="ISSVQQEEYYIVFGALSLKSSLEDILLEITSLVDNALDLAEITE
                     EYSH"
BASE COUNT         2416 a         2480 c         2230 g         2184 t
ORIGIN      
        1 aaaatagtgt caggattaat caaaaccaat cacatattga ttcgaattga actatcacga
       61 aggtaaatgg cgatgagtaa agtgaaaagt atcacccgtg agtcctggat cctgagcact
      121 ttcccggagt ggggtagctg gctgaatgaa gaaattgagc aagagcaggt cgcaccaggc
      181 actttcgcaa tgtggtggct cggctgtacc ggaatctggt tgaaatcaga aggcggtgcc
      241 aacgtttgtg ttgatttttg gtgcggtact ggcaaacaaa gccacggtaa cccgttaatg
      301 aaacagggtc accagatgca gcgcatggct ggcgtgaaaa aactgcagcc aaacctgcgc
      361 accaccccgt ttgttcttga tccgtttgcg attcgccaga tcgacgcggt actggcgact
      421 cacgatcaca acgatcatat cgacgttaac gtcgctgctg ccgtgatgca gaactgtgct
      481 gatgacgtac cgttcattgg accgaaaacc tgcgttgatt tgtggattgg ctggggcgta
      541 ccgaaagagc gttgcatcgt cgttaaaccg ggcgacgtag tgaaggtaaa agacattgaa
      601 attcatgcac ttgatgcttt cgaccgcacg gcactgatca cgctgccagc cgatcaaaaa
      661 gcggctggcg tactgccgga cggcatggac gatcgcgcgg taaactacct gttcaaaacg
      721 ccgggcggtt ccctgtatca cagcggcgat tcccactact ccaactatta tgcaaaacat
      781 ggtaacgagc atcagatcga cgtggcttta ggatcgtacg gagagaaccc acgtggtatc
      841 accgacaaaa tgaccagtgc tgatatcctg cgtatgggcg aagcgctgaa tgcgaaagta
      901 gtgatcccgt tccaccacga tatctggtca aacttccagg ccgatccgca agagatccgc
      961 gtgctgtggg agatgaaaaa agatcgcctg aagtatggct tcaagccgtt tatctggcag
     1021 gtcggcggca aatttacctg gccgctggat aaagacaact tcgagtacca ctatccgcgt
     1081 ggtttcgatg attgcttcac tattgaacca gatctgccgt tcaagtcgtt cctgtaatct
     1141 gtcgcttgta ccggatagcg tggagatgct cgcgctatcc ggcatagtat ttcctggcta
     1201 tttcaaatat catctaaaaa aatcaaattt tatcggaata gctcatgact gaagcacaaa
     1261 gacatcaaat cctcctggaa atgctcgcac aattgggctt tgtgaccgtt gagaaagtcg
     1321 ttgagcgtct gggaatttcg cctgccactg cacgacgcga tatcaataaa cttgacgaaa
     1381 gcggcaaact gaaaaaagtg cgcaatggcg cagaagctat tacccaacag cgcccgcgct
     1441 ggacgccgat gaatctgcat caagcgcaga atcacgatga aaaagtacgt attgctaaag
     1501 cggcctcgca gctggttaat ccgggcgaaa gcgtagtcat caactgcggc tccaccgcgt
     1561 ttctgcttgg gcgggaaatg tgtggcaagc cagtgcaaat catcactaat tatctaccgc
     1621 tggcaaatta cctgatcgat caggagcatg acagcgtgat cattatgggc ggacagtaca
     1681 acaaaagtca gtccatcact ttaagcccgc aaggcagcga aaacagtctc tatgccgggc
     1741 actggatgtt taccagcggc aaaggactga cagcagaagg gttgtataaa accgatatgc
     1801 tgacggcaat ggcagagcag aagatgctga gcgtggtagg gaaactggtg gtactggttg
     1861 atagcagtaa gattggcgaa cgcgcgggaa tgctttttag ccgtgccgat caaatcgata
     1921 tgctgatcac cggcaaaaat gctaacccgg aaatcctgca acaactggaa gcgcaagggg
     1981 tcagcattct gcgtgtttaa agatgctggc ggaaaaatgt cacggcagca tctaacgcct
     2041 caggcgtaat gcggtgacgc acgcctggct gccatgaaca ggttagcagt ttatcccgtc
     2101 ccgtctcgct taaggcctgc tgcaaacgta gtgattcgtc ggcaggcacg acgtcatcgt
     2161 caaggccatg ccataagagc agcggtctgt tccccaactc tgtcagatgg tttgtcgctt
     2221 cccactctgc cagcggcgcg acaatgttat tgaattcatt ctgctgtgct gccgtttcag
     2281 gtatcagcgg tggaaacagt gaacgggcga gcgatgtaaa atagcccgat cccatcatgc
     2341 tggcggtaca ttttaccgtg gggtggcgag cggtaattcc cagtgccgtc atcgcgccca
     2401 tcgacgcgcc accgactgcc agacggtcat cacgcagcca gttttcttcg gctattgccg
     2461 cacgtaaggt agtgaattcc tgcatacttt gtagcaagat ttgccagaat tgatttaacc
     2521 gccgcgctgc gtcgccacta aaacggctac cgtgatcggg cgcatccggc atgatcaccc
     2581 gcaaaccagc ctgcgccagc gcaacggcaa aatagctata caccagactg gacgaagtaa
     2641 agccgtgata aaaaatcaca cacggtaacg gggtatcttt ttgcccgaca ggataagcat
     2701 gaagaacggg aatatctgcc agctcgcgtg attctatttc aatcatctgt ctctcctttt
     2761 tgtctggtat gacatgaaga aatccttagc acaagtttct gctggcggta ttagttcata
     2821 atgttgagat gtgggttacg ctttcgtcac gttttcattc gaaaatcgcg taccaggtaa
     2881 caattcggga acattccccc aaagttaaat taataaggca ctacactatg gttagcagga
     2941 aacgtaatag cattatttac cggtttgcca gtttattatt ggtgttgatg ttaagtgcct
     3001 gtagcgcact gcaaggtacg ccacagccag caccgccagt aacggaccat ccgcaagaga
     3061 ttcgccgtga ccagacgcaa gggttacagc gaataggtag cgtaagcacg atggttcgag
     3121 gttctccgga tgacgcatta gcagaaatca gagcgaaagc tgtcgctgca aaagctgatt
     3181 attacgttgt cgtcatggtg gacgaaacca tcgtgacagg acagtggtat tcacaagcca
     3241 ttttgtaccg taaataaatc agacaactaa catagtcagc tttacattgc tttgcgtccc
     3301 tatgcgttta cctgtgcaca ataaattacc ggctggaagc tcgggcgttt attaccgcga
     3361 tggaatgccc tgcaacatgt ggggaaacga aaaatggagc tgacgatgaa acaattactt
     3421 gcctcaccct cgctgcaatt agtgacttat cctgcgagcg ccacggcaca gtctgcagaa
     3481 ttcgccagtg ctgactgcgt gacgggcctg aatgaaatag gtcagatctc agttagcaat
     3541 atttcagggg atccgcagga cgtggaacgt atcgtggcgt taaaggccga tgaacaaggt
     3601 gcgtcatggt accgcattat taccatgtac gaagaccagc agcctgataa ctggcgcgta
     3661 caggcgatac tgtatgcgtg agtgtcatct acagcaaatg aacaatgccg gatgcggcgc
     3721 gagcgccttg tccggcctac aattttaaaa tatttcattt agttcatcga aaccagcgtc
     3781 gcatcagtcg tatacgctta caaacacact cctcccgtcg cccgcagcaa taaatcattc
     3841 tggatctgct ctgacaaccg tacgccgccg cgcgtatcta acatcacctt acaccacgtc
     3901 tgcgccattg gcggagaggc atatttcaac atttgcgcac cgcagcccag caggaatagc
     3961 tgatgagtaa tctctcgccc caattcttca gctggcttac gcagctgctg ctgtaaacga
     4021 cgaaccgcgc gatcaaaata gcgatcctgc cctttcactt ccacaaatgc ttccgacaat
     4081 aagtcgtata cgcccgcttg cttattgaga acgcgcagca catccaggca cataatattg
     4141 ccggaacctt cccaaatact gtttaccggc atctcccggt aaagccgcgg cagctcgctc
     4201 tcctcgcaat aaccaatgcc gcccagcacc tccatcgctt cggccacaaa cggaatgcca
     4261 cgtttgcaga tcacaaattt cgccgcaggc gtaaataaac gcgcccacag agcttctttg
     4321 gcatcggcgc gccggtccca cgctcgcgca agacgaaaca gcaacgccgt ttgcccttca
     4381 agctgaagtg ccatgcgact taagacatga cgcataaggg gctgttggat caatggatta
     4441 ccaaaaacat ggcgttgatg tgcatgataa atcgccagcg aaaatgcacg gcgcatcatg
     4501 gcatggctac ccagggcgca atcaaaacgc gtcatcccac ccattttcag gatcagacga
     4561 attccttccc cttcctgccc caacaaccag ccaatggcat cctgaaattc cacttcgcag
     4621 ctggcgttag agcgattacc cagcttatct ttcagccgct cgaggcgaat cgcgttgcgt
     4681 tgcccgtcag gcaaaaagcg cggcacaaaa aagcaggaca aaccgcccgc ggtctgtgcc
     4741 agcaccagat gcgcatcgct ttgcggcacc gagaaaaacc atttatgccc caccagccga
     4801 taagaaccat cgtccagacg ctctgcgcgg gtggtgttgc tcatgacatc ggaaccgccc
     4861 tgcttttccg tcattcccat gccgatcaac aagccacgtt tttgcccacc tggcaataag
     4921 tgagaatcgt agcgatcgct caacagcggc gtggtccagt cctgaaacgg cgcgggtaac
     4981 atctgtaaca acaatggcgt ggcggcaaag gtcatcgtta tcggacataa cgacccagcc
     5041 tcaacctgcg catgtaacat aaaacgcgcc gcgcgcgcca caaatgcgcc ggagcgagcg
     5101 tcttcttccc aggcaagatt gtgcacccga ttggtacata gcgcctgcat cagcaggtgc
     5161 caggcggggt gaaaacgcac atcgtccaga cggcgtcctt gcgcatcgta gcgcaataat
     5221 tcaggcggat tcacattcgc cagccgcccc agttcaaggg attcagccgt tcctaactgc
     5281 tgaccaatac tggcgagaaa atcgctatcc cagccagcac cttcacgcgt taccgcttcg
     5341 caaagcgcgc catcagacag gtataagttg ctgttattta atggtatagg ttgattaaaa
     5401 acggtgtgag tttgccagtg cactgtgtct ccctccatca atggcagtca ccattagtat
     5461 ggtcactgcc atggattcat gacagaaaga gattgctaaa acattcttat ccagcaatgt
     5521 aatgcggaac gaaacgtgag gtgtctttgg tgatcagcgt gttatcttca cggatcccca
     5581 ttccgcacgc ttcatcatcg acaatccagc taccgatgag tgtgtagcta tcgccaaatc
     5641 gcggcagagg ttgaaacgcc tggtagatca tcggttcatc ggcgtaatca ccatcagcgt
     5701 ggtcaacaac gttattctga ccgtcgaaaa tggtgacgtt tccgccttcg cgcgagtaaa
     5761 tcggtttacg cacatagctt tcgccagcgg caatctgcgg tttttcacca tcgaaccagg
     5821 atgcaagaag gttaggatga ccagggaaga agcgccaaag cagcggcatt agccctttat
     5881 tactcaagat acttttccat aacggctcca cccattgctc acgacgcttg cgcagcagcg
     5941 gaccgttatc gtcacgcatc atccactcca gcggatacag cttaaatgca cgctggatga
     6001 cattatcatc aagatcggtc agtacgccgc cgacgcccaa accgagctct tcaatgtaga
     6061 taaaccgaga ctcctgccct gcctgctcgg cgcagtcctg caagtacagc acggtactcc
     6121 tgtcttcatc ggtatcctga cagcagcaaa aataaaacgg ctcccggcta taaagctcgc
     6181 taaagcgtga aatcaggcgc tcctgaatgg cattgtactg atcggcatcg cgcggaataa
     6241 cgccgctgcg ccgggcatct tccagccaca accactggaa ataagccgac tcgtacaatg
     6301 aagttggcgt atcggcgttg tactccagca acttaaccgg tgcattacca caccaggcaa
     6361 aatccatacg gccatacaac gaaggatcgc gtgcgcgcca gctttcggcg atcacatccc
     6421 agtacaacgg cgggatcgcc agttgcgtca ggatctcttc atctttaacc gcacgatcca
     6481 ccacctccag gcacatctga tgcagttccg cagtcggttt ttcgatctgc tcttcaatct
     6541 ggcgcagagt aaagcggtaa gcccgactct catcccaata gatttcattg tcgatgatat
     6601 gaaagtcaaa tccgttatcg gcggcaatcc ggtccagatc ccgccgcaca ggaacgttgt
     6661 gtctcagcat gattagcctc cccaatgccc acgggcgctg gaagaacgac catagccgcc
     6721 gcgagaaacg gttgacgctt ttttggtggt aaagccgccc gaagagtaag actctttttt
     6781 gccagaaccg gagcgccagg agtaatcacc ggaagtgttg cgccagacgg ggcgggaagc
     6841 aaatgaggat ccgccgctgt tatagacgaa cggctcatcg cgatctttac gaataacccg
     6901 gcttaacaaa aatccagaaa cgaccgggat ccaactctgt tcaacattgt cgtagtaaca
     6961 attttcatac ttagactgac aattctgctg agtcatattc ttcggaacat cggcataaaa
     7021 tgccgctttg gcgttgttcc agccatgagc acagatatct gaattattac catcatcaat
     7081 acaatcctgc acagtcgcgt aaaacgttcc gtcgccgtcg ttatcgacat cgccactatc
     7141 gccacaacct ttcaatacga aaaaagcggc accgcccatt agcgccaacg ttaagtattt
     7201 ttgtgcgtag cgattgcggc gcggctcaaa aggattatgc ggtctaccaa tgcgactgat
     7261 tgccccatgt ccgatatttt tgttatttct ggatttgcgt tttctggcca tgatcgtcca
     7321 ttaccatgtc atacaagctg cgttaaaaat accgccagcc agcgcggcgg ttcccataaa
     7381 cattccggct gcggtattgt gattaataat tttttcgctt aatgcgggca tataaagcct
     7441 gacgccagca aaaacgagta attgaatcac cagagcaatc ccgccccatg cgaaatagtc
     7501 tggaatactc accgcattga ttgccgcact ggataagggg atcacgtacc ccaacaatgt
     7561 tccgctgaac gccagtgccg ccgcggtgtt attgtttttg attaactgcc attcgttgtg
     7621 cggtgtaatt ttggagtaga taaacaggaa aataatcacc atcgccacgc caataaaaaa
     7681 ataggcacta aaagcaagaa gtgaatccag tatatgcatt gtaaacgtcc ttgtaattaa
     7741 ccaataatat gaagtgatgt cagcgggata tcgataccca gcgcgcgcga aaataaccac
     7801 tccggctcgc cgagatcgtt aaaagattcc tcaccattta acagcaaata ttcgtaagta
     7861 tctccggtca cctgccgttg gtagcccatg ctaaaattat gcacttccca tttggcatgg
     7921 ttttgatttt ctactttttc cagcatgtag acgggttcga tatttcccgg ttcttcgctg
     7981 ttaaaaaatc gctgccagcg tttttcctgc cagtttaagg tcattgcccc catcgctttg
     8041 gcgttgatcg cctcgcgcca gtgactctct ttactgatac cgtagctctc ttcatagaca
     8101 aagagcttga tatcatcaat gtcatcaata tcttcgccgc cagtggtatt gatttgcagg
     8161 aactcatcgc ccgaagtgta gtaacggaaa atctggctac ccccgcccag atcgatgtgg
     8221 ctgacagcgg ctaccgtaaa ttcctcaccc ggcagcgcaa tcagcaattc atcttccagc
     8281 aaacgaaacg ataatgtgtc gagcgtaaag ccactattga gatgcagacc cagcggaccg
     8341 cgagcgatag caggcttatt atccttgcca aacagacgct ggaaaaaacc agacatattt
     8401 tatccttaaa atagttacca gccttgcagt gaggctggca aaaaattact cgccctgttg
     8461 gcgttgcagt cttgctaata catcctgggc gctactttta ttgctaccgc caattccagc
     8521 ttccgccagc ttttcatcga gatcgcgacc gtccgcgact ttttccaact gtgcggcagc
     8581 atccaggcga gcctgacgtt cggcctgacg cgtttgcagg cgttttaagg attctgccgc
     8641 tgtcgaaacg ctggaagatg cgccaacggt agaggtcgtt acagcctgtt gtgcacgctg
     8701 catggcttca gtggctttaa cgacttccat ttgctgctca aactgagcga tacgctgcgc
     8761 tgtcgctgta actgcttttt ccacgccatc acgagagact tccagattcg acaacacttg
     8821 ctcttcagcg gtaatgagat tctcaaggcg cgcgatttct tcagcaactt cgttaatcaa
     8881 cgacggatta acgttcttgc ttaacgcttc cagcgcacgc gcttccagac tggctttgcg
     8941 ctcacgtaaa tctttcagct tatcgtgact taatttcacc cgtgccagca gatcaacgcg
     9001 agattttccg gctttatcga gttcagcttt agcgtcacga atatgctgtt ccagcatgcg
     9061 cacgccctgg gtttcttcaa tggactcttc cgcctgggag ataaacgatt tccccagcgt
     9121 aaataaactt tttaaaattc ccataaatac ccgtccttgt aattagtgtg aatattcttc
     9181 tgtaatttca gccagatcca atgcgttatc taccagcgag gttatctcaa gcaggatatc
     9241 ttcaagagaa gatttaagcg ataacgcacc gaaaacaatg taatactctt cctgttgcac
     9301 gctggagatc
//
LOCUS       DACADF010000167         9270 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.169, whole genome shotgun sequence.
ACCESSION   DACADF010000167 DACADF010000000
VERSION     DACADF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.169"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(146..2413)
                     /gene="adiA"
                     /locus_tag="ISB30_004071"
     CDS             complement(146..2413)
                     /gene="adiA"
                     /locus_tag="ISB30_004071"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAP1991648.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKINESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2612..3520)
                     /gene="melR"
                     /locus_tag="ISB30_004072"
     CDS             complement(2612..3520)
                     /gene="melR"
                     /locus_tag="ISB30_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAP1991649.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAILNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3803..5158
                     /gene="melA"
                     /locus_tag="ISB30_004073"
     CDS             3803..5158
                     /gene="melA"
                     /locus_tag="ISB30_004073"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP1991650.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEDYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(5155..5805)
                     /locus_tag="ISB30_004074"
     CDS             complement(5155..5805)
                     /locus_tag="ISB30_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAP1991651.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWLAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(5928..7574)
                     /locus_tag="ISB30_004075"
     CDS             complement(5928..7574)
                     /locus_tag="ISB30_004075"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAP1991652.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(7652..8992)
                     /locus_tag="ISB30_004076"
     CDS             complement(7652..8992)
                     /locus_tag="ISB30_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAP1991653.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         2200 a         2290 c         2488 g         2292 t
ORIGIN      
        1 caaaaaaaag cgcggggtgg gaccgcgcaa aaagcatgac attttttgtt tgcagcaatt
       61 tttatgaaaa cgttgccata cttaaaaggc ctcaagccag gcggcgtaat gttatttaac
      121 aattacgcct tcagcggaat agtggttacg ctttcacaca cataacgtgg taaataccgt
      181 caataatttc tgtcccttct gtttcgtgtt caaatccagg gaaatgatga tcccaggatt
      241 gcagcgagcg taaataactt acttgcggac tgtttttatc gccgaagttt tcaccagaca
      301 gcagcatcgg gattcctggc ggatacggga taactgagtt tgccgcgata cgtcctggca
      361 gattttcaat ggataccagt tcgacattgt tgtcgacaat cgcgttgtac gcttcacgcg
      421 gggtgatttc cgccaccggt aggccggaat aggcttcatt caaccgtgcg ccagggttgt
      481 tttctttcag ccaggcaaac atggtgtcac ccagatcgtg aatccccatg ttcgcgtaag
      541 tgtcaggata ttgttcaaca agttccggca tcacctgcgc cagcggtgtg ttggcgtcat
      601 agtggcgttt gaaggagcaa agggtgttaa ccagagttcc ccatttccca cgggtcacgc
      661 ccatagagaa caggaacata atctggaagt cagtggtacg ggtaggcaca atgccgtggc
      721 gaccaagcca ggctgtgacc agcgccgccg gaacaccggt ttcttccagt tcaccatctt
      781 cacccattcc cggagcaagg atgctgactt taatcgggtc gagcatactc cagttatccg
      841 gaatatcttt gaagccgtgc cagctttcgc ccggatgcat gacccagcag tcctgaacgg
      901 tggtcagcag tttggttggt gcgtcagcaa agtcataggt tttgccggtt tgtgggtcgg
      961 tgacgacttc tttgttccac ggtttgaaga accagctacc gtcagcggtg aactctttat
     1021 atagccgcgc catcgcctga cggaaatcaa ccgcttcgtc gatcacttcc tgggtcagtg
     1081 acaggccgct gttgccgtcc atcatcgaca ccgccacgtc gttggatgcg cagatggcat
     1141 acagcgggga ggtggtggca tgcatcatgt aggcctggtt gaagcgggag aagttaatcg
     1201 ccccacgacc ttcacgtaca tgaatataag aggcctgtga cagcgcattc agcagtttgt
     1261 gggtggagtg ggtggcgaaa acggtaggac cgttgtgatc gccaggttcg ccgcgcatgg
     1321 catagtgatc ggcatagatc gggttgaaac gtgcatagcc gtaccaggct tcgtcaaagt
     1381 gcagacgatc ggaggttttt tccagcagat cctgcgcttc tttagcgtta taacacacgc
     1441 cgtcataggt acagttggtc accacgcagt aagacggttt ttgcccggct ttgtctttag
     1501 tcagcgggct ttcattgatt ttcttctgca aggtttcagg ttgcatttcc tgcggataga
     1561 ttggcccgat aatgccgtag cggttgcggc ttggcaccat gtagaccggt ttcgcacctg
     1621 tcagcatcaa gccttgttcg atggatttat ggcagttacg gtcaacgacc acgacatcgt
     1681 tatcggtcat gcaagcctgc atgatggtgc ggttagagcc ggaagtaccg acgactaccg
     1741 accaggagcg atcggcacca aatacgcgtg cggcatattt ttcgctttcg ccaaatgcgc
     1801 cagtatggtc aagcaaagaa ccgagggaag tgcgttcgat gcccatgtcg gtgcggaaca
     1861 gattttcacc atagtagtca tggtagaaac gtccggcggg tgttttggta aaaccaacgc
     1921 cgccctggtg gcctggcgct gcccaggaat attcatggat gtcgctatat ttcatcagcg
     1981 cgctgaacag tggcggcaat agctgctggc ggtagcgggt catcgcggca acggcgcgtc
     2041 cggcgataaa gtcggcggta tcttccagaa tccaggcgaa ttcatcgaca agctccagca
     2101 ggtcgcgatc cattgcggcg agggcttttt cccgatcgcc caacaggaag accggcacgt
     2161 tttgttggcg ctcatgaagc ttaccgatca attgtctgac gttttgatgt tcgtccggat
     2221 gttccatttg atagctgaac atcaggcagt caatggcttc gtttgaagag agaatggcaa
     2281 aaccatcatc aaaggaggtg gatttaatca cggtaacatt ttgctggctt aaagcatctg
     2341 ccagacgctc aacggcgtta ccgacccagg tgtcttgatg gagaaactcg ctttcaacaa
     2401 ttaatacttt catcattgct tacccggtta tgaaggaatc ttcgttgcgg gcaagtatct
     2461 tccggccttt tcgggctgta aagcgcgtga aaaacaaaca tttttgaaat atttattttt
     2521 ataaacaata tgttatgtga ttattttaaa ttattttcac tgcttattat cgacggctaa
     2581 actatttttt tggctgatac tgatatcgtc tttagccggg aaacgtctgg cggcgctgtt
     2641 ggctaagttt gcggtattgt tgcggcgaca tgccgacata tttgccgaac gtgctgtaaa
     2701 aacggctact cgaacgaaag cctgccgtca gggcaatatc gagaatactt ttatcggtat
     2761 cgctcagtaa tgcgcgaacg tggttgatgc gcatcgcggt tatgtactgt ttcatcgtca
     2821 attgcatgac ccgctggaat atccccattg catagttggc gttaagtttg acgtgctcag
     2881 ccacatcgtt gatggtcagc gcctgatcat agttttcggc aataaagccc agcatctggc
     2941 tgacataaaa ttgcgcatgg cgcgagacac tgtttttgtg tgtgcgcgag gttttattga
     3001 ccagaatcgg ttcccagcca gagaggctaa atcgcttgag catcaggcca atttcatcaa
     3061 tggcgagctg gcgaatttgc tcgttcggac tgtttaattc ctgctgccag cggcgcactt
     3121 caaacgggct aagttgctgt gtcgccagtg atttgatcac catgccgtga gtaacgtggt
     3181 taatcaggtc tttatccagc ggccaggaga gaaacagatg catcggcaga ttaagaatcg
     3241 ccatgctctg acaggttccg gtatctgtta gttggtgcgg tgtacaggcc cagaacagcg
     3301 tgatatgacc ctgattgata ttcacttttt cattgttgat caggtattcc acatcgccat
     3361 cgaaaggcac attcacttcg acctgaccat gccagtggct ggtgggcatg atatgcggtg
     3421 cgcgaaactc aatttccatc cgctggtatt ctgaatacag cgacagtggg ctacgggtct
     3481 gcttttcgtc gctgctgcac ataaacgtat ctgtattcat ggatgactct ctttcctgga
     3541 atatcagaat tatggcagga gtgagggagg atgactgcga ataggcgcac ggttttattc
     3601 ctctccctgg aggtgcgagg agagcggatg agaagattgg ggtcgattat tcagcttttc
     3661 aggtaattta ttcccataaa ctcagatcta ttgatgtgcc acttaggata tgagtttatg
     3721 ggaatgctca acctggaagc cggaggtttt ctgcagattc gcctgccatg atgaagtcat
     3781 tcaagcaagc caggagatct gcatgatgtc tgcacctaaa attacattta tcggcgctgg
     3841 ttcgacgatt ttcgttaaaa atattcttgg tgatgtgttc catcgtgagg cgctgaaaac
     3901 ggcgcatatt gccctgatgg atatcgatcc cacccgcctg gaagagtcac acattgtggt
     3961 gcgcaagttg atggattcag caggggccag cggcaaaatc acctgccaca cccaacagaa
     4021 agaagcctta caggatgctg attttgtggt ggtggcattt cagattggcg gttatgaacc
     4081 ttgcacggtg actgatttcg aggtctgtaa gcggcatggt ctggaacaaa ccattgccga
     4141 tacgctgggg ccgggcggca ttatgcgtgc gctacgtacc attccgcacc tgtggcagat
     4201 ttgcgaggac atgacggaag tctgccccga tgccaccatg ctcaactacg ttaacccaat
     4261 ggcgatgaat acctgggcga tgtatgcacg ctatccgcat atcaagcagg tagggctgtg
     4321 ccattcggtg cagggaacgg cggaagagct ggcgcgtgac ctcaatatcg atccggctac
     4381 gctgcgttac cgttgcgcag gtatcaacca tatggcgttt tacctggaac tggagcgtaa
     4441 aaccgcagac ggtagctacg tgaatctcta tcctgaattg ctggcggctt atgaagcagg
     4501 gcaggcaccg aagccaaata ttcatggtaa tactcgctgc cagaatattg tgcgttacga
     4561 aatgttcaaa aagctgggct acttcgtcac agaatcgtca gaacattttg ctgagtacac
     4621 accgtggttt attaagccag gtcgtgagga tttgattgag cgttataaag taccgctgga
     4681 tgagtacccg aaacgctgcg tcgagcagct ggcgaactgg cataaagagc tggaggatta
     4741 taaaaacgcc tcccggattg atattaaacc gtcacgggaa tatgccagca caatcatgaa
     4801 cgctatctgg actggcgagc cgagtgtgat ttatggcaat gtccgtaacg atggtttgat
     4861 tgataacctg ccacaaggat gttgcgtgga agtggcctgt ctggttgatg ctaatggcat
     4921 tcagccgacc aaagccggta cgctaccttc gcatctggcc gccctgatgc aaaccaacat
     4981 caacgtacag acgctgctga cagaagctat tcttacggaa aatcgcgacc gtgtttacca
     5041 cgccgcgatg atggacccgc atactgccgc cgtgctgggc attgacgaaa tatatgctct
     5101 tgttgacgac ctgattgccg cccacggcga ctggctgcca ggctggttgc accgttaagg
     5161 ggtaatcgct tccggcgctc tggtgattaa gccaaagcgc cgtagctggc ggcaatgaaa
     5221 gcgagcgagg aatatcaccg tcgttaatgc gccaagcagc gcgcagaaca tatcggattg
     5281 cgtgtcccac tggtcgccct gggtgccgag aaaatcatca gctccctggc ccattgccag
     5341 cgctgccaac cactcgatta attcatacat cgcgcttatc gccagcgcca cgcagcagac
     5401 cagaaacgcc accatcttac gtccgcgaac gtacatcccg cgtacaagga tctctcgtgc
     5461 caccagtgca ggcaccagcc cctggaaaaa atgtcccagt ttgtcatacg gattacggct
     5521 caaccccagc cattcctgca cctcaaaacc aacggggact ttcgcgtagg tgtattgccc
     5581 gccgaccatc aggatgatgg cgtgaaagaa aatgagcgta taaagcagcg gcgttaacgg
     5641 ataacgttta gcggtggcaa gcagtagcgg cacgacaata atcaccggtg tcacttccat
     5701 cagccaggtg agtttgtcat gggccgaaat gccggtataa atcaggatta acgttagcgc
     5761 cagtgcgcca gtgttaagaa ttaacggctt gagtgtgcgg gtcatggtga gttcgcaagt
     5821 cagggaaaat actgactatt caccagcaag cgtgaaatta caatcgcaaa agaggtggcc
     5881 cagggggatc acctggcagc atgctgccag gcgctgggcc gaagaggtta cttagtgcag
     5941 ttcgcgcact gtttgttgac gatctgctgg aagaagtcgt tacctttgtc atcgaccagg
     6001 ataaacgccg ggaaatcttc tacttcgatt ttccagatag cttccatacc cagctccgga
     6061 taagcgacgc attccagatg cttgatgctc tgctgcgcca gtactgccgc cggaccgccg
     6121 atactaccga ggtagaagcc gccgtgttta tggcacgcgt cggtaacctg ctgactgcgg
     6181 ttacctttcg ccagcatgat catgctgccg ccgtgggatt gcagcagatc cacgtaggag
     6241 tccatacgac ctgcggtggt tgggccaagt gaacctgatg gataaccggc aggcgttttc
     6301 gccggacccg cgtagtagat cgggtgatct ttgatgtact gcggcagttc tttaccggcg
     6361 tcaatcagct ctttcagctt ggcgtgtgca atatcgcgac ccacgataat ggtgccggtg
     6421 agcgacaagc gggtggatac cgggtattgc gaaagctggg caaggatctc tttcatcggg
     6481 cggttaaggt cgactttcac tgcttcgcct tcacctgcct gacgcagttc ttgtggaatg
     6541 tactggcctg ggttgtgttc cagtttttcg atccagatac cttcgcggtt gattttcgct
     6601 ttaatgttac ggtcagcgga gcaggagact cccatgccta ccgggcagga tgcgccgtga
     6661 cgtggcagac ggataacgcg gatgtcgtgc gcgaagtatt taccgccaaa ctgcgcgcca
     6721 agaccgagtt tctgtgcctc ttccagcagt tcctgttcca gctggatatc gcggaacgcc
     6781 tgaccatgtt cgttcccttc cgtcggcagt tcatcgtaat agtgagcgct tgctaacttg
     6841 acggttttca ggttggtttc cgcagacgta ccgccaatca caaacgcgat atggtacggc
     6901 gggcaggctg cagtaccgag ggtacgcatt ttctcgacga ggaagttttt cagtttgccg
     6961 ggcgtcagca gggctttggt ttcctggtag agatacgttt tgttggcaga gccgccgcct
     7021 ttagcgacac agaggaattt gtactcatcg ccatctaccg cgtacaggtc gatttgcgca
     7081 ggcaggttcg tgccggtgtt gacctcttta tacatgtcca gcggcgcgtt ctgtgaatag
     7141 cgcaggttat cttcgatata ggtgttatag acgcctttcg acagtgcttc ttcatcaccg
     7201 ccaccggtcc acacgcgctg acctttttta ccgacgatga tcgcggtgcc ggtatcctgg
     7261 caggtcggca gcacgccttt ggcggcgatt tcggagtttc ttaagaattg cagcgccacg
     7321 tacttgtcgt tttcgctggc ttctggatcg tgaagaatag ccgcaacctg tttctggtgt
     7381 gccgggcgga gcatgaaaga agcatcgtga aaggcttgct gcgccagcag ggtcagggct
     7441 tctggttcca ctttcaggat ggtttcgccg tcgaagtcgg caacgctaac gtaatcggaa
     7501 gtgagtagat aatattcggt attgtctttc cccatcggga aaggtgcctg gtagataaag
     7561 ggtttgtttg acatagcttc cagcctgtaa ctctgtattt gttattcgaa aatggcgtgc
     7621 cgcgtagtta cgggcggcac gcaaagtgca tttataagaa cccgtacatc gcggcgaaga
     7681 tccagccgaa gacgcacgat acgctcacac caatcaaccc cggcagaata aagctgtggt
     7741 tgatgacgaa gcgaccgatg tgggtggtgc cggaacggtc aaactgaatc gctgccagat
     7801 cgctcggata agtcggcagg atgtaataac cgtagcaagc cggtgctgaa gccacgatgt
     7861 atgccggatc aacgccgatc gccagcgcga ccggaacaat cgccgccagc gccgcagcct
     7921 gagagtttac aaacttggaa accagcagca gaacaatggc ataggcccac ggatactctt
     7981 tcaccatttc acccagtacg ccctgaattt cagacatatg cgcaccgaac atggtttcag
     8041 ccatccatgc gataccgtac accgccacga tggcgatcat accggaacgg aagacttcgt
     8101 tttttgagat agacgcggga ttggttttgg tcaggataat aatcagcgcc ccggtcagca
     8161 gcataaacat ctgaataacc agtaccatcg acagcggttt gccgccgaag gatggacgca
     8221 ggtccgaatc agcaccaaga agggcgacta cagcgattgc cccgaggaag atccacattg
     8281 ccagccagtt gcttttcggc agttttttat ccagcagcgt cgcggtatca ccgtaaacat
     8341 actcacggtt ttccggtacg gagatgaatt tctggaactc ttcgtcttta tccagatctt
     8401 taccgcggaa ccagctgaag ataccgatcg ccaggatacc gattaacgtc gatggaatgg
     8461 tgattgccag caggtcgagg aactcaagat ggcgaccatc aaaggtgaca ttacccagca
     8521 tcgcaaccag agatacgacc gcaaccgaca ccggactggc gataatcccc atctgtgcac
     8581 cgatagaact tgccgccatc ggacgttccg gacggatgtt gttcttaata gcaacgtcgt
     8641 agatgatcgg cagaatggtg taaaccacat gacccgtacc gcaaagaatg gtcagcgtac
     8701 aggtcacaaa cggcgcgaca attgagacat atttcgggtt gcgacgcagc agcttctcgg
     8761 caatttgcag catgacatca agaccgcccg aagcttgcaa ggtcgccgat gccgccacca
     8821 ccgcaatgat aaccagcatg acatcaaccg gtggtttacc tggctgaagg tggaagacga
     8881 agaccagaat gaccagaccg ataccgccta ataaacccag cgcgatacca ccctttctgg
     8941 caccataaaa cagacatatc agtattatga taagttggat agtaaataac atgtgtgaac
     9001 cctcgcgata atcctattta aatttttgct gaatagatca cagtcacgtt ctgttttgta
     9061 tgaactgttt tcagagcctg actaaatatc cgtttggtcg ttaccggctt gagcaaatac
     9121 ctcacagtga atattggctg attaaagcag cggcaaattt atgcactgtg ataagcggct
     9181 ttttcagaga gaaagactct ctggcgatgg gtttatagtt tgtacagcgc agatgttaat
     9241 gttttgtttg ctgttatctt attgatattt
//
LOCUS       DACADF010000168         9154 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.170, whole genome shotgun sequence.
ACCESSION   DACADF010000168 DACADF010000000
VERSION     DACADF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.170"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            466..1368
                     /locus_tag="ISB30_004077"
     CDS             466..1368
                     /locus_tag="ISB30_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAP1991654.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTDAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISRQGENFIQVDF
                     DTPWCQPESNVVAELSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTAPEWIVDKVAHYGG"
     gene            1372..1676
                     /locus_tag="ISB30_004078"
                     /pseudo
     CDS             1372..1676
                     /locus_tag="ISB30_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1753..2436
                     /locus_tag="ISB30_004079"
     CDS             1753..2436
                     /locus_tag="ISB30_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAP1991655.1"
                     /translation="MSRFVLGNCIDVMTRIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            2437..2658
                     /locus_tag="ISB30_004080"
     CDS             2437..2658
                     /locus_tag="ISB30_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991656.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            2672..3106
                     /locus_tag="ISB30_004081"
     CDS             2672..3106
                     /locus_tag="ISB30_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAP1991657.1"
                     /translation="MYGTCETLCRELAAKYPGDAPLMLVIWSPEEIQALADGMEISLT
                     DHEIRTVLARMEDIPEDQRTESGISSAAVMEIISNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARTLLKNNTHENN"
     gene            3157..3921
                     /locus_tag="ISB30_004082"
                     /pseudo
     CDS             3157..3921
                     /locus_tag="ISB30_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001476318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3806..4377
                     /locus_tag="ISB30_004083"
                     /pseudo
     CDS             3806..4377
                     /locus_tag="ISB30_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
     gene            4473..4775
                     /locus_tag="ISB30_004084"
     CDS             4473..4775
                     /locus_tag="ISB30_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP1991658.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            4822..5244
                     /locus_tag="ISB30_004085"
     CDS             4822..5244
                     /locus_tag="ISB30_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAP1991659.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            5241..5432
                     /locus_tag="ISB30_004086"
     CDS             5241..5432
                     /locus_tag="ISB30_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991660.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            6467..6697
                     /locus_tag="ISB30_004087"
     CDS             6467..6697
                     /locus_tag="ISB30_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991661.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            6749..8110
                     /locus_tag="ISB30_004088"
     CDS             6749..8110
                     /locus_tag="ISB30_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HAP1991662.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYISDMKITEI
                     PQK"
     gene            8158..8721
                     /locus_tag="ISB30_004089"
     CDS             8158..8721
                     /locus_tag="ISB30_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP1991663.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWLPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            8747..8977
                     /locus_tag="ISB30_004090"
     CDS             8747..8977
                     /locus_tag="ISB30_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991664.1"
                     /translation="MPVCPRREKTRAGGAERGRAHAPPLSLRGRSDGGFCFFMPVICL
                     LLMLLFLLLLLLLLLLLLLLLLLLLFFFFLLL"
BASE COUNT         1911 a         2547 c         2756 g         1940 t
ORIGIN      
        1 tcccactcgt gttgtcggtc tgattatcgg tctgggacca cggtcccact tgtattgtcg
       61 atcagactat cagcgtgaga ctacgattcc atcaatgcct gtcaagggca agtattgaca
      121 tgtcgtcgta acctgtagaa cggagtaacc tcggtgtgcg gttgtatgcc tgctgtggat
      181 tgctgctgtg tcctgcttat ccacaacatt ttgcgcacgg ttatgtggac aaaatacctg
      241 gttacccagg ccgtgccggc acgttaaccg ggctgcatcc gatgcaagtg tgtcgctgtc
      301 gccggcctcc tcacccggtc acgtttcgtc gttcctcctc cacgcgctgc ggcttcgggg
      361 ccgcacctgc attcgtatgc ggtcgcccgg ttacaggtgc ggcacggcct gatggaggcc
      421 gcatgtgaga ggagaattcc catgccaaac tggtgctcga atcgtatgca tttttctggt
      481 gaaccggcac agattgctga gattaaacga ctggccagcg gtgccgtcac accgctttat
      541 cgccgcgcca caaatgaagg tattcagctg tttctggccg gaagtgccgg acttctgcag
      601 accactgaag atgtgcagtt tgaaccgtgc cccggactga cggatgccgg acgtggtgtt
      661 gtatcgccgg agaatatcgc gttcacccgc tggctgacac acctgcagaa cggtgtactg
      721 ctggatgagc aaaactgcct gatgctgcat gaactctggc tgcagagtgg tactggccag
      781 cgtcgctggg aaggattacc ggatgaggtc agggaaacca tcaccgtgca tttcaccgca
      841 aaaagaggtg actggtgtgg cttctggagt aacgaggatg tatcggtgtg gtggaaccgt
      901 ctgtgtgaca atgtactgcc ggaaaaaacc atgccgtttg acctgctgac ggttctgccc
      961 acccgcctgg atgttgaagt gaatggcttt aacggtggtg ttctgaacgg tgttccttct
     1021 gcgtatcact ggtatacgga acagtatggc gtgaagtggc ctgtggggta tgaggtgaat
     1081 atcagccgtc agggagaaaa cttcattcaa gtggatttcg acacgccgtg gtgtcagccg
     1141 gaaagcaacg ttgttgcaga attaagccgc cgtttcggct gcacgctgga gcactggtac
     1201 gccgaacagg gctgcaattt ctgtggctgg cagcgatatg agcgtggaga gcttgttgat
     1261 gtgctgtggg gggaactgga atggtcttcc ccgacagatg atgatgagct gccggaagtc
     1321 accgcgccgg agtggatagt cgacaaggtg gcgcattacg ggggatgaag tatgacggag
     1381 acgggcgggc aaccgcccgt ttcttttccg acaaaggatg tcgccgtgct cctcttttta
     1441 ctgcgccggc tgacgcggcg cggcaggaac gctgcctgtg gtcagtgtcc cgcgtccggt
     1501 gggcacggga cgggcgcttt tactgctccc ggctggcacc ggtgacagtg tacgccagcc
     1561 tgtcgccctt ttctgatgaa cgcccttcaa gccgctttcg cggcatattc tcgccgtcaa
     1621 aaagacgacg gctgcgatat tccacggtcg gcctgacccg ttaccggacg cggtgaacag
     1681 cccacaggca gcggggaacg ggcaccacag gggggccctc ccggtgccct ctgtaagaga
     1741 aggagcgttg ttatgtcccg ttttgtcctt ggtaactgca tcgatgttat gacgcgtatc
     1801 cctgataacg ccattgattt catattaact gacccgccat atctcgtcgg ttttcgtgac
     1861 cgttccgggc gcaccatcgc cggcgataaa accgatgaat ggctgcaacc ggcctgtaat
     1921 gaaatgtacc gcgtactcaa aaaagacgcg ctgatggtga gcttctacgg ctggaaccgc
     1981 gtcgatcgct ttatggccgc ctggaaaaat gcgggattca gcgttgttgg tcacctggtc
     2041 ttcaccaaaa actacacatc gaaggccgca tatgtgggct atcgccacga atgcgcctac
     2101 atcctggcaa aaggccgtcc acgtctgcca caaaacccgc tgccggacgt gctgggctgg
     2161 aaatattctg gcaatcgtca tcacccgacg gaaaagcccg ttaccagcct gcaaccgctg
     2221 attgagagct tcacacaccc gaacgcaatt gtgctggacc cgtttgcagg cagcggctca
     2281 acctgcgtcg ccgccctcca gtccggacgc cggtatatcg gtatcgagct gcttgagcag
     2341 tatcaccgtg ccgggcagca acgccttgcc gccgtgcaac gggccatgca gcagggggcc
     2401 gcgaatgatg actggtttat gccggaggct gcgtaaatga actatgccgg acacgaaaaa
     2461 cttcgcgccg acgtggcgga ggtggccaac accatgtgtg accttcgggc caggctgaac
     2521 gatatggagc atcgctgtcg ctttgattcc gatgtgcttg ttgaacgcct gacacgccag
     2581 acgctgtatc gcgccaatcg cctgtttatg gaggcgtata ccgaaattct ggaactggat
     2641 tcctgcttca aagattgaga aggagacgag aatgtacgga acatgcgaaa cgctatgccg
     2701 ggagctggca gcaaagtatc cgggagacgc gccgctgatg ctggttatct ggtccccgga
     2761 agagattcag gcactggctg acggtatgga aatttctctg accgatcatg aaatcaggac
     2821 cgtcctggcg cgcatggagg acatcccgga agaccagcgg actgaatccg gtatttcttc
     2881 cgccgcggtg atggagatta tcagcaacgt gagcgaaaac cgccaggtga ccgtccctgc
     2941 tgaactgctg gcgtccctga ttcagaccgc tgaacaggca ctgtggaaac gtgagtgggc
     3001 cgcccgtgat aacggcctcg ccgtcccgga atgcgttacc cgccgtcagg cggtggttaa
     3061 tcaggcccgc accctgctga aaaacaacac acacgaaaac aactgatgtt atcgccgcct
     3121 gccctgcggg cggcgataca gggagaacgc ttaatcatga acgaaaacag cacgctgaac
     3181 gcactgattt gtcgtcacgc ccgtaacctg ctgctggcgc agggctggcc ggaagagacg
     3241 gatgttgacc agcgaaatcc gaactatccg ggctggatca gcatttacgt gcggctggat
     3301 gcgccccggc tggcgacgtt acttatcaac cgtcacggcg gcgtactgcc gccgctcctg
     3361 gcctccgcca ttcagagact gaccggaacc ggggcggaac tggtactgtc cggcagtcag
     3421 tggcagtcgc tgccggtact tccggcagac ggaacgcagg tgtctttccc gtatgccgga
     3481 gaatggctga ccgaagacga aatcagggct gttcttgatg cggtgcacga tgcggtacga
     3541 agcatctgtt accaggtggc agaagatgcg cggcgtatcc gtgcggcgct gaccaccacc
     3601 ggtcagacgt tgctgacccg ccagacgcgc cgctttcgcc tggtcgtgaa ggaaagcgat
     3661 cacccctgct ggctcgatga agatgacgaa aacctgcccg tggtgctcga tgccatcgtg
     3721 aaccggggag cacgtttttc gtcggtggaa atgtatctgg tcagtgagtg tattgagcat
     3781 atcctgtcca gtgggctggc ctgcgatgtg ctgcgtatac cggatgaacc gccccgccgc
     3841 tggtttgacc gtggtgttct gcgggaggtg gtccgggaag cccggaccga aatccgcagc
     3901 atggcggatg ccctggcaaa aatccggaaa tgatgacact ggcggggtaa atccccgcct
     3961 ttttcctgcc ggtccccgga cagagcacaa aggaagtcgc cgccggctcc gctttacccg
     4021 gccatgcagg gcatggcctt gtggtttttc agttatgtgg cctcagcgtc gtgtgcgggc
     4081 tgtgccgtgc ctccatctta gccgggctgg cgttgatgca agggtacgct tcgccgctgc
     4141 ggtcacccgg tccctccttc ccgtctgccg tgattttccg gtcgttcacc cgctcagatt
     4201 tcgcggtctc accctgcaac tcccgtcagc cgtgtgcgtc aggctgcggc ttcccttgcg
     4261 cccctgcatc cccgcctgtt cgcccggctt ttatggaggc acggcaccgc ccggtgtcgc
     4321 ggaatatgtg aacaacggag gaaaacatca tgcaatatgc gaaacctgtc actctgaatg
     4381 ttgaagagtg cgaccgtctc tcctttctgc cgtatctgtt tggcaacgat tttctgtatg
     4441 ccgaggcgta tgtgtacgcg ctggcgcaaa aaatgatgcc ggaatatcag ggaggcttct
     4501 ggcacttcat ccgcctgccg gacggtggcg gttacatgat gccggatggc gaccgtttcc
     4561 acatggtgaa cggtgcaaac tggtttgacc gtaccgtgag tgctgatgcc gcaggcatca
     4621 tcctgacctc ccttgtgatt aaccgccagt tgtggctgta ccacgacagc ggggatgcag
     4681 gactgaccca gctttaccgg atgcgcgatg cgcagttgtg gcgtcacatc gaatttcacc
     4741 ccgaatgcaa cgcgatttac gcggctctgg actgattaac ggacggggcg gcaatgccgc
     4801 ccctgtaaag gagacgagaa catgtactgt actgttaaag aaattatccg cgaagtgctg
     4861 gatacagacg tgccggacag tgaatgcgtt tttgccgtag tgctgacccg tggggatgtg
     4921 cgtcacatag cccaggactg gagtctgaca gacgatgagc tggaaaccgt catgcagcgg
     4981 ctggacgatg cctttgaata tggtgcggat gtcagcgttg ttcacggcgt tgttcgtgaa
     5041 ctgatggaag aaaagcgcgc cagccgtcag gtgacagtcc cggcggtgat gctggaaaaa
     5101 gtgatggcgc tggcaggcag tgaaatgaag cgcctgtatg ccgtcgggag tgagaacggg
     5161 ggcgacggtg acgcattcgt cagggaggaa cgcgaagcaa tggacgttgt gttacaggcg
     5221 ctggacgggg agacgatgtc atgaatatca gcacagaaac ccgcgaaatt ctgcgtaatt
     5281 acagggccgt gattaatgcg cggcgtcgtg aaatggggca gaaaccgctc accaccgcgc
     5341 agattgttga tgaaatctgc gattttgtgg cgaatcagca ggcggttttc ctgggcggtc
     5401 actacatctt acagggtagc agaaacaggt gatacagggg caggcggtgg aggccgcctg
     5461 tcagtccgcg cgaaaacccc tggcaggctg acgcctgccg gaagggaagc gggccacgct
     5521 ggcgcgtcgc ccgttaccct tcccgtctgt gatgtgtctg actcaggagt gtttctttct
     5581 tatgttatgg aactgttcct gatgttgttg ttcctttatc tgctgctctg attctgacta
     5641 ctgcagtgtt gttctgtctg ccgggcgcac cttcggctcc cggctgcctg gcggcatccg
     5701 gtcgtcaggc ccgactccga caggcagctg ctggcgcacc tgccagtctc cgccgaaccc
     5761 gctggcgcgg tttcgggctg tcgctacggt ccgatgcgtg aaaaccccgg gcttgcagcg
     5821 accgttccgg tcgcgccacc cttgtcgcgc cgtggcctgc gctcccggca tcccggcaaa
     5881 aggccgccgg gatgtaaggg gcaacgccgt ttcgcgcacg cgctctccgt cgttgcggcg
     5941 ggtccctgct cattgcctgc cggtgccaca cacccgtatg agtccctgtc gcctggggct
     6001 gatacagcca caacctgccg cactgactgt ccagggtaaa tgaacagccc acaaaatttt
     6061 ttacccaaaa cccggtaaaa aattttgcgt tctgcccagg acaggtgcgt caggccgtgg
     6121 cagtgatgcc ccttgcggcg aaggttctca aacgggtgaa gttcatacca cagggtatac
     6181 agcagcaggt tcccgcgaaa cgtcaaaacc gcggctcccg aaactcaacg ggtgatgttc
     6241 tttaaaaatc aggatttatt tcaggctccg gcaatacgca tttatgtgtt ctgtcagtca
     6301 tccggctggc aggtaatgag aacgcaggaa cagaaggagg aaaacaccgg agaacgtcag
     6361 acgcagatat gaaaaaagca ggtcagggac ctgcttaatc cacaccgcag agactgcacg
     6421 aacagtcttt acggtttcat taattaacgt cataagaggc taaattatga cagtaagtat
     6481 tgtttcacct tccgcagcgg ctgtaaagcc ccgccgtcat ccccgtttca gacgcgaaga
     6541 catccccgcg ccggaaattg accccgtgct gaaagccttc gggcgacaca ttgcccggag
     6601 ttttcaccgg gggcgtggtg tacacatccc cgccatgaaa aacacggcct tcgggcaggt
     6661 gctccgcaca ctcgaactga aacgcgcttt taactgatga ggtgagccgc cccgcgaggg
     6721 gcggcaacag acggaaggag aacattttat gacattagca gcaatcacca tgaccgcccc
     6781 ggaagccgcc agccctgtgc agatgtaccg cgcgacctac tcaccggatg acaacaaact
     6841 gcgcctgtat gccgtgtcac gtcttgaccc ggagacgtat aaaaaagtgc atgatgccgg
     6901 tttccgctgg gcaccaaaac aggcgctgtt tgtcgcgcca gcctggacac cgggccggga
     6961 agacgtgctc ctctcacttg ccggagagat tgaggatgaa gacagcacgc tcgctgaacg
     7021 tcaggaagca cgggcggagc ggtttaccgg atacagcgga aagcgggcca gtgaatccgc
     7081 acaggcactt gatgaagtgg aaagactggc cgcgatgatc ccgcccggtc agcccattct
     7141 tgtggggcat cacagcgaac gccgcgcccg tcgtgatgcg cagcgtattg aaaacggcat
     7201 gaaacgagcc gtgatgctct ttgaacgtgc ggaatactgg gaagagcggg cgcggtcggc
     7261 actgcttcac gcgaagtata aagaacgtcc ggacgttcgc tggcgtcgta tcaaaaaaat
     7321 cgaagctgat ttgcgcaagg ctgaaaagac catcgcgcag tcgcagaaat atctgacgat
     7381 gtggcgggct gaatcgctgg atctgaatat ggcaaaactc atcagcagtc atgaccatat
     7441 cagcgcctgt ttcccgctgg atacgtatcc gcgcccggca gaaaaaagcc agtatgaagg
     7501 aagtcgatcg ttatggtcgg ccctggatga tgacatcatc accacggagc aggcccgcga
     7561 aattgcgatc cgctgtcatg aacggcagat tcagcatcaa caacgctggg ttaaccacta
     7621 tcagaaccgc ctgatctatg agcgtgccat gctggacgaa agcggcggcg tggttacccg
     7681 gacacaggat tttgagccgg gcggacaggt tttcagccgg ggcgagtggc tgaccatcat
     7741 ccgtgtgaac aaaagcaacg gggcggtgag ttcagtcaca acgccaaatt acagttttct
     7801 cgggtacagc ggcacgatga aagtgacgcc cgatcgcatc acggactaca aagcaccatc
     7861 ggcagaagag gctgccgtcg ccagtcaggc cgcgaagcgt ccgccggtag tcaactatcc
     7921 gggggaaggt ttccgggaaa tgacaaaggc acagtgggcc gccctgcccc gggactgtaa
     7981 ggccgtgcgc agtgtggcag aagcagaaga ccacggggca taccggtacc gccgcacaat
     8041 ggacaataat ttccgcctgg tgaatgtgta tatctccgac atgaaaatca cggaaatccc
     8101 acagaaataa ggtatatccc cccgggaaaa tccccggggg atgacggaga taatgatatg
     8161 cacagtcagt taagagaacg tatccggctg atgcgcgcaa ggctggataa cgccgcgccg
     8221 gttgctgaaa tccgggctga atctcagctt tttgtgactc ctgccccggt ctgtgatcgc
     8281 ctggtgatgc tggcggagat cagcaaccgt gatcacattc tggaaccctc tgccggcacc
     8341 ggggccatac tgcgggcgat tcgggatacc gcgccggggg ccatgtgtga tgcggtggaa
     8401 atcaacagcg ggctggtccg gtatctgcgg gaaaatttta acggtgtcag ggttcagtgt
     8461 ggcgacttca tggaatggct gccggtgcag tattacagcc gggttatcat gaatccgccg
     8521 ttcagccacg ggcaggatat ccggcatatc ctgcgggcct tttccctgtt gcgtccgggt
     8581 ggcgtgctgg tcgccgtctg tctcaacgga ccacgccagc aggagaagct gttaccgttt
     8641 tctgacgtcc gcgaggagct gccgcgcggc acgtttgctt ataccgatgt cccgacgatg
     8701 attattcgtc tgcgtgcctg atgacacagg gccggcggga ggataaatgc ctgtctgccc
     8761 gcgccgggaa aaaacgcgcg ccggtggcgc tgaaaggggc cgcgctcacg cgccgcccct
     8821 ttcattacgc ggacgttctg atgggggctt ctgtttcttc atgcctgtca tttgtctgtt
     8881 actgatgttg ttgttcctgc tactgctact gctactgcta ctgctactgc tactgctact
     8941 gctactgcta ctgttcttct ttttcctgct gctctgattc tgactactgc agtgttgtcc
     9001 tgtctgccgg gcgcaccttc ggctcccggc tgcctggcgg catccggtcg tcaggcccga
     9061 ctccgacagg cagctgctgg cgcacctgcc agtctccgcc gaacccgctg gcgcggtttc
     9121 gggctgtcgc tacggtccga tgcgtgaaaa cccc
//
LOCUS       DACADF010000169         9014 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.171, whole genome shotgun sequence.
ACCESSION   DACADF010000169 DACADF010000000
VERSION     DACADF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.171"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            22..1164
                     /locus_tag="ISB30_004091"
     CDS             22..1164
                     /locus_tag="ISB30_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1991665.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1171..1935)
                     /gene="glcC"
                     /locus_tag="ISB30_004092"
     CDS             complement(1171..1935)
                     /gene="glcC"
                     /locus_tag="ISB30_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAP1991666.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLEFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2186..3685
                     /gene="glcD"
                     /locus_tag="ISB30_004093"
     CDS             2186..3685
                     /gene="glcD"
                     /locus_tag="ISB30_004093"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HAP1991667.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTLKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3685..4737
                     /gene="glcE"
                     /locus_tag="ISB30_004094"
     CDS             3685..4737
                     /gene="glcE"
                     /locus_tag="ISB30_004094"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAP1991668.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMI
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4748..5971
                     /gene="glcF"
                     /locus_tag="ISB30_004095"
     CDS             4748..5971
                     /gene="glcF"
                     /locus_tag="ISB30_004095"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HAP1991669.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNDVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            5976..6380
                     /locus_tag="ISB30_004096"
     CDS             5976..6380
                     /locus_tag="ISB30_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HAP1991670.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6402..8573
                     /gene="glcB"
                     /locus_tag="ISB30_004097"
     CDS             6402..8573
                     /gene="glcB"
                     /locus_tag="ISB30_004097"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAP1991671.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVTFKVVDKQLRIQLKNGKETTLRTLAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQSEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8928..>9014
                     /locus_tag="ISB30_004098"
     CDS             8928..>9014
                     /locus_tag="ISB30_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease"
                     /protein_id="HAP1991672.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFF"
BASE COUNT         2152 a         2273 c         2576 g         2013 t
ORIGIN      
        1 ttatttgtta tccacagaga tatggaatgc gatctgctga tgattaaaat tgaaaaagta
       61 attaataaaa atgaccttaa ggcatttatc gcttttccgt catcacttta tcccgacgat
      121 ccaaactgga tacccccttt attcattgaa cgcaacgagc atttgtctgc gaaaaatccg
      181 gggacggacc atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg
      241 cgtattaccg cgcaaataga taccttgcac cgcgagcgtt acggcaaaga taccggtcat
      301 ttcggcatga ttgacgccat tgatgatccg caggtttttg ccgcgttgtt tggcgcagcg
      361 gaagcgtggt tgaagtcaca aggtgcaagt aagatcagcg gtcctttcag cctgaatatc
      421 aatcaggaaa gcggattact gattgaaggt ttcgatacac caccctgtgc gatgatgccg
      481 cacggtaaac cgtggtatgc cgcgcatatt gaacaattgg gttatcacaa aggcattgat
      541 ttactggcgt ggtggatgca gcgaaccgat ctcactttct ctccggcgct aaaaaaactg
      601 atggatcagg tgcgtaagaa ggtgaccatt cgctgcatca atcgccagcg gtttgccgaa
      661 gagatgcaga tcctgcgtga gattttcaat tctggctggc agcacaactg gggatttgtg
      721 ccgtttaccg aacatgaatt cgcgaccatg ggcgaccaac ttaaatatct ggtgccggac
      781 gatatgatct acatcgctga gattgattct gcaccctgcg cgtttattgt cggcttgcca
      841 aacatcaacg aggcgattgc cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa
      901 ttgctgtggc gtttaaaagt cagcggtgtg cgaactgcgc gagtaccgct gatgggcgtg
      961 cgggacgagt atcagttcag ccgcatcggc ccggtgattg cgttgttatt gattgaagcc
     1021 ttacgcgatc cgtttgcccg ccggaagatc gatgcgctgg agatgtcatg gatcctcgaa
     1081 accaatacag gcatgaataa catgctggaa cgcattgggg cggagccgta caagcgttac
     1141 cgattgtatg aaaagcagat ttgatggcag tcacccctgc ttcatctcca gcggcaccgc
     1201 cgagcgaatc aaatggtggc cttccagctc gatatcgtgg agattctttt tcacggtccg
     1261 cacatgatcg cgtgctgcgc gctgggcgac gtgcggcagc cgctgcaaca ccgcgttgta
     1321 gatccgcgca tgctggcgat cgatctgctt tttttgctgt ggtcgatggt agagattatt
     1381 tacgctggca aacaccgaat taaacatcag gtcggtcaat gattgcagcg taaactccag
     1441 cacctgattg tgagaagcct gacaaatggc gagatggaaa gcgtgatcca actgcgcatg
     1501 ttcgatcagc gaaatctctt tgttgttctc actggcggcg agcatttttt cataacagcg
     1561 ggttatcaca acaaaatcag cctgggttcc cagcgttgcc gccagccttg ccgattcgcc
     1621 ctccagtaat gcgcgaacgt cgagcagatc gtacagcgtt cgcggctgcg tactgaacag
     1681 atggatcagc gggctggtgt cctgcacccg attaagccgt gcgacacgag aatcgcgacc
     1741 ctgcgccgtt tcaataatcc cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga
     1801 gcgtgagaag ccgagctttt cacacagtcg acgttccgag ggaagcggct gaccgacctt
     1861 cagtacgccg tcgataatta accgttcgat actctctgca accacttcgc aaatagggcg
     1921 acgttcatct ttcattcccg gacctcgtgc acaggtagga ccaatttttc tgttcttaac
     1981 tcgcaaaaca cgcacatcac gtaagtgatt atgttacatc aatttaacat taagttaacc
     2041 aagacaaggt cacagagctg gaaaaaaaat ggtctgaccg gtagctaaag agatagacga
     2101 aaacgaaaag cccgcttaat aactgttcac agaagcagcg cgaaaaaatc agctgccaca
     2161 caacacaaca aagcgaagcc tactcatgag catcttgtac gaagagcgtc ttgatggcgc
     2221 tttacccgat gtcgaccgca catcggtact gatggcactg cgtgagcatg tccctggact
     2281 tgagatcctg cataccgatg aggagatcat tccttacgag tgtgacgggt tgagcgcgta
     2341 tcgcacgcgt ccattactgg ttgttctgcc taagcagatg gaacaggtga cggcgattct
     2401 ggctgtctgc caccgcctgc gtgtaccggt ggtgacccgt ggtgcaggca ccgggctttc
     2461 tggtggcgcg ctgccgctgg aaaaaggtgt gttgttggtg atggcgcgct ttaaagagat
     2521 cctcgacatt aaccccgttg gtcgccgtgc gcgcgtgcag ccgggcgtgc gtaacctggc
     2581 gatctcccag gccgttgcgc cgcataatct ctattacgcg ccggatccgt cctcacaaat
     2641 cgcctgttcg attggcggca atgtggcgga aaatgccggt ggcgttcact gcctgaaata
     2701 tggtctaacc gtacataacc tgctgaaaat tgaagtgcaa acgctggatg gcgaggcgct
     2761 gacgctggga tcggacgcgc tggattcgcc tggttttgac ctgctggcgc tgtttaccgg
     2821 atcggaaggt atgctcggcg tgaccaccga agtgacgtta aaactgctgc cgaagccgcc
     2881 cgtggcgcgg gtgctgttag ccagctttga ctcggtagaa aaagccggac ttgcggttgg
     2941 tgacatcatc gccaatggca ttatccccgg cgggctggag atgatggata acctgtcgat
     3001 ccgcgctgcg gaagatttta tacatgccgg ttatcccgtc gatgccgaag cgattttatt
     3061 gtgcgagctg gacggcgtgg agtctgacgt acaggaagac tgcgagcggg ttaacgacat
     3121 cttgttgaaa gcgggcgcga ctgacgtccg tctggcacag gacgaagcag agcgcgtacg
     3181 tttctgggcc ggccgcaaga acgcattccc ggcagtgggg cgcatttccc cggattacta
     3241 ctgtatggat ggcaccatcc cacgccgcgc gctgcctggc gtactggaag gcattgcccg
     3301 tttatcgcag caatatgatt tacgcgttgc caacgtcttt catgccggag acggcaacat
     3361 gcacccgtta atccttttcg atgccaacga acccggtgaa tttgcccgcg cggaagagct
     3421 gggcgggaag atcctcgaac tctgcgttga agttggcggc agcatcagtg gggaacatgg
     3481 cattgggcgc gaaaaaatca atcaaatgtg cgcccagttc aacagcgatg aaatcacgac
     3541 cttccatgcg gtcaaggcgg cgtttgaccc cgatggtttg ctgaaccctg ggaaaaacat
     3601 tcccacgcta caccgctgtg ctgaatttgg tgccatgcat gtgcatcacg gtcatttacc
     3661 tttccctgaa ctggagcgtt tctgatgcta cgcgagtgtg attacagcca ggcgctgctg
     3721 gagcaggtga atcaggcgat tagcgataaa acgccgctgg tgattcaggg cagcaatagc
     3781 aaagcctttt taggtcgtcc tgtcaccggg caaacgctgg atgtgcgttg tcatcgcggc
     3841 attgttaatt acgacccgac cgagctggtg ataaccgctc gtgccggaac gccgctggtg
     3901 gcgattgaag cggcgctgga gagcgccggg caaatgctcc cctgcgagcc gccacattat
     3961 ggtgaagaag ctacctgggg cgggatgatc gcctgcgggc tggcggggcc gcgtcgcccg
     4021 tggagtggtt cggtccgcga ttttgtcctc ggcacgcgca tcattaccgg cactggaaaa
     4081 catctgcgtt ttggtggtga agtgatgaaa aacgttgccg gatacgatct ctcacggtta
     4141 atggccggaa gctacggttg tctgggtgtg ctcactgaaa tctcgatgaa agtgttaccg
     4201 cgaccacgcg ccaccctgag tctgcgtcgg gaaatcagcc tgcaagaagc catgaatgaa
     4261 atcgcccagt ggcaactcca gccattaccc attagtggct tatgttactt cgacaatgcg
     4321 ttgtggatcc gccttgaggg cggcgaagga tcggtaaaag cagcgcgtga actgctgggg
     4381 ggcgaagagg ttgcgggtca gttctggcag caattgcgtg aacaacaact gccgttcttc
     4441 tcgttaccag gtaccttatg gcgcatttcc ttacccagcg atgcgccgat gatggattta
     4501 cccggcgagc aactgatcga ctggggcggg gcgttacgct ggctgaaatc gacagccgat
     4561 gacaatcaaa tccatcgcat tgcccgcaac gctggcggtc atgcgacccg ctttagtgcc
     4621 ggagatggtg gttttgcccc gctaccggct cctttattcc gctatcacca gcagcttaaa
     4681 cagcagctcg acccttgcgg cgtgtttaat cccggtcgca tgtacgcgga gctttaagga
     4741 gcaggctatg caaacccaat taaccgaaga gatgcggcag aacgcgcggg cgctggaagc
     4801 cgacagcatc ctgcgcgcct gtgttcactg cggattttgt accgcaacct gcccaaccta
     4861 tcagctcctg ggcgatgaac tggacgggcc gcgcgggcgc atctatctga ttaaacaggt
     4921 gctggaaggc aacgacgtca cgcttaaaac acaggagcat ctcgatcgct gtctcacttg
     4981 ccgcaattgt gaaaccacct gtccttctgg tgtgcgctat cacaatttgc tggatatcgg
     5041 gcgtgatatt gtcgagcaga aagtgaaacg cccactgccg gagcgaatgc tgcgcgaagg
     5101 attgcgccag gtagtgccgc gtccggcggt cttccgtgcg ctgacgcagg tagggctggt
     5161 gctgcgaccg tttttaccgg aacaggtcag agcaaaactg cctgctgaaa cggtgaaagc
     5221 taaaccgcgt ccaccgctgc gccataagcg tcgggtttta atgctggaag gctgcgccca
     5281 gcctacgctt tcgcccaaca ccaacgcggc aactgcgcga gtgctggatc gtctggggat
     5341 cagcgtcatg ccagctaacg aagcaggctg ttgtggcgcg gtggactatc atcttaatgc
     5401 gcaagagaaa gggttagcgc gggcgcgcaa taatattgat gcctggtggc ccgcgattga
     5461 agcaggtgcc gaggctattt tgcagactgc cagcggctgc ggcgcgtttg tcaaagagta
     5521 tgggcagatg ctgaaaaacg atgcgttgta tgccgataaa gcgcgtcagg tcagtgagct
     5581 ggcggtcgat ttagtcgaac ttctgcgcga ggaaccgctg gaaaaactgg caattcgcgg
     5641 cgataaaaaa ctggccttcc actgcccgtg taccctacaa catgcgcaaa agctgaacgg
     5701 cgaagtggaa aaagtgttgc ttcgtcttgg atttacctta acggacgttc ccgacagcca
     5761 tctgtgctgc ggttcagcgg gaacatatgc gttaacgcat cccgatctgg cgcgccagct
     5821 gcgggataac aaaatgaatg cgctggaaag cggcaaaccg gaaatgatcg tcaccgccaa
     5881 cattggttgc cagacgcatc tggcgagcgc cggtcgtacc tctgtgcgtc actggattga
     5941 aattgtagaa caagcccttg aaaaggaata acaaaatgaa aactaaagtc attcttagcc
     6001 agcaaatggc gagtgcaatt attgccgcag gtcaggaaga ggcgcagaaa aacaactggt
     6061 ctgtttccat tgctgttgcc gatgacggcg gtcatctgct ggcgttaagt cgcatggacg
     6121 attgcgcgcc gattgcggct tacatctccc aggagaaagc gcgtaccgcc gcgctggggc
     6181 gtcgtgaaac taaggggtat gaagagatgg tgaacaacgg acgtaccgcg ttcgtgactg
     6241 cgccgttatt aacgtcgcta gaaggtggcg taccggttgt tgtggatggg caaattattg
     6301 gtgccgtggg cgtttctggt ttaaccggag cacaggatgc gcaggtcgcg aaagcggcag
     6361 cagcggtgtt ggcgaaataa gcgaaaacga ggagagaaac aatgagtcaa accataaccc
     6421 agggccgttt acgcattaac gccaatttta aacgttttgt ggatgaagaa gttttaccgg
     6481 gaacagggtt ggacgctgcg gcgttctggc gcaattttga tgagatcgtt cacgatctgg
     6541 caccagaaaa tcgtcagttg ctggcagaac gcgatcgcat tcaggcggcg cttgatgagt
     6601 ggcatcgcag caatccgggg ccggtaaaag ataaagcggc ctataaatct ttcctgcgtg
     6661 aactgggcta cctggtgccg caaccggagc gcgtaacggt ggaaaccacg ggcattgaca
     6721 gcgaaatcac cagccaggcg gggccacagc tggtggttcc ggcaatgaac gcccgctacg
     6781 cgctgaacgc ggcgaacgct cgctggggct cactgtacga tgcgttatac ggcagcgaca
     6841 tcatcccgca ggaaggggcg atggtcagcg gctacgatcc gcagcgcggt gagcaggtta
     6901 tcgcctgggt tcggcgtttc ctcgatgaat cactaccgct ggaaaacggc agctatcagg
     6961 atgtggtgac gtttaaggtg gttgataaac aattacgcat ccagttgaaa aatggtaaag
     7021 aaaccacgtt acgtaccctg gcgcagtttg tcggttaccg tggcgatgcc gctgcgccga
     7081 cctgcatttt gctgaaaaat aacggcctgc atattgagct gcaaattgat gccaacggac
     7141 ggattggcaa agacgatccg gcgcacatca acgatgttat cgtcgaagct gccatcagta
     7201 ccattctcga ctgcgaagat tcggtcgcgg cggttgatgc ggaagataaa atcctgctgt
     7261 accgcaatct gctgggtctg atgcagggga ctctgcaaga gaaaatggaa aaaaacggtc
     7321 ggcaaatcgt gcgtaaactg aatgacgatc ggcagtacac cgccgccgat ggctctgaaa
     7381 tttctctgca cggacgctcg ctgctgttta tccgcaacgt ggggcatttg atgaccattc
     7441 ctgtgatttg ggacagcgaa ggcaatgaaa tcccggaagg cattcttgat ggcgtcatga
     7501 caggcgcgat tgccctctat gatttaaaag tgcagaaaaa ctcgcgcact ggcagcgtct
     7561 atattgtgaa accgaaaatg cacggtccgc aggaagtggc gttcgccaac aaactgttta
     7621 cccgcgttga gacaatgctc ggtatggcac cgaataccct gaaaatgggc attatggatg
     7681 aagaacgccg aacctcgctg aacttgcgta gctgtattgc tcaggcgcgc aaccgcgtgg
     7741 cgttcatcaa taccggtttc ctcgaccgta ccggcgatga gatgcattcg gtgatggaag
     7801 ctggcccgat gctgcgtaaa aaccagatga aatcgacgcc gtggatcaaa gcctacgagc
     7861 gtaataacgt gctttccggt ctgttctgtg ggctgcgcgg taaagcgcaa atcggtaaag
     7921 gcatgtgggc aatgccggac ctgatggcag acatgtacag ccagaagggc gaccaactgc
     7981 gtgccggggc aaacacagcc tgggttccgt caccaaccgc tgctacgctc catgcgctgc
     8041 actaccacca aaccaacgta cagagcgtgc aggccaacat tgcccagagc gagttcaatg
     8101 ctgaatttga accgttgctg gacgatctgc tgactattcc ggtggctgaa aacgctaact
     8161 ggtcggtgga agagatccaa caagagctgg ataacaacgt gcaggggatt ctggggtacg
     8221 tggtgcgttg ggtggagcag gggattggtt gttcaaaagt gccggatatt cataacgtgg
     8281 cgctgatgga agaccgcgca acgctgcgta tctccagcca gcatatcgcc aactggttac
     8341 gtcacggtat tctgaccaaa gaacaggtgc aggcgtcgct ggagaatatg gcgaaagtgg
     8401 ttgatcagca aaacgctggc gatccggctt atcgtccgat ggcggggaat ttcgctaact
     8461 cgtgtgcttt taaagctgcc agcgatttaa tcttcctcgg cgtgaaacag ccaaacggct
     8521 ataccgaacc gttattacac gcctggcgtt tacgcgaaaa agaaagtcat taatagcgtc
     8581 aaaaaagccc cggcaagttt tattgtcggg gctatgatag taactgcggt agtacattta
     8641 tcccggaaat aaccggagcg attatcaggg tttaacaata ttaaataccc gtagcgcatt
     8701 actagcgggc ctaaaacaac aatatggaca tcttaacgat attctgcgcc tgcgtgtgcg
     8761 gaacagaaac acttacccga tatacttgtt atgccagagg tagcaattgc gttagcagcg
     8821 tactgttgct gtaaagtata acgcctatat tttacagttc agtcctcttt tgcttattac
     8881 aaccttaagt atatcaagca tataaagata ataagagact gaacaatatg gttacctgga
     8941 cccaaatgta tatgccgatg ggaggactgg ggctatccgc tctggtcgcc ctgatcccga
     9001 taatattctt cttc
//
LOCUS       DACADF010000170         8995 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.172, whole genome shotgun sequence.
ACCESSION   DACADF010000170 DACADF010000000
VERSION     DACADF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.172"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..317)
                     /locus_tag="ISB30_004099"
     CDS             complement(<1..317)
                     /locus_tag="ISB30_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991673.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFMGAPQKTESMV
                     TPV"
     gene            complement(346..1043)
                     /locus_tag="ISB30_004100"
                     /pseudo
     CDS             complement(346..1043)
                     /locus_tag="ISB30_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1105..1227)
                     /locus_tag="ISB30_004101"
     CDS             complement(1105..1227)
                     /locus_tag="ISB30_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991674.1"
                     /translation="MRFRFSLSGTWLTICNIIIKYNYASYFYSQNANNVAYTPV"
     gene            complement(1299..2321)
                     /locus_tag="ISB30_004102"
     CDS             complement(1299..2321)
                     /locus_tag="ISB30_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HAP1991675.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLSEDRRTLLLTFTVNNEAHLRAQ
                     IIRRFGFIPEGIRVMTWFEFLHGFCFRPFLQEQLASRGLSFDQPPPRIPRTNARHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRIRLARYYDELLVDEVQDFAGHDFNFLLELCRAEI
                     TVLCCGDFYQHTFDTSHDGNVNSTLHDDITRYEARFDAAGFAVDRDTLNRTWRCSASV
                     CEFITGQLNIRIAAHGIHASLIETIADTERSATLHADNTVIKLFYREHHRYGCYSMNW
                     GASKGLDHFQDVCIVMGSSHWKLLTRQELATLPPSSRNRLYVACSRARGNIYFVPETH
                     LRRFRN"
     gene            complement(2306..3868)
                     /locus_tag="ISB30_004103"
     CDS             complement(2306..3868)
                     /locus_tag="ISB30_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP1991676.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFTHDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRQFQEGERRADQLPVLTADVFLSNGGDPDLNGR
                     QNLAGINADGLRMRIAPMTEEYGQDIHNVLQQDPDNFPYEYYSVRFSTFSGGHFASFR
                     RYLRHLLLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCTRHLSAI
                     NDTLETYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGEL
                     HVLLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGSARP
                     VLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYRRLYGRAPEED
                     GVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVICSRSRVFADR
                     DNTRSTFEISLYQDNADLCDTLFRGPRRTLTVQEYMLANKAEAAFRLLQLHAGELTVP
                     DYIQEALAWIRE"
     gene            complement(3942..4358)
                     /locus_tag="ISB30_004104"
     CDS             complement(3942..4358)
                     /locus_tag="ISB30_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HAP1991677.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITY
                     SEMRFGATGPKASPRHVELVDAFCARLDAILPWDRAAVDATTEIKVALRMAGTPIGPN
                     DTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR"
     gene            complement(4355..4585)
                     /locus_tag="ISB30_004105"
     CDS             complement(4355..4585)
                     /locus_tag="ISB30_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAP1991678.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFAQFEKADPDFMAEREDVVSDEGRFNL"
     gene            4542..5003
                     /locus_tag="ISB30_004106"
     CDS             4542..5003
                     /locus_tag="ISB30_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991679.1"
                     /translation="MARLLPFLKMDTVLIISSWPTGLYDYVQYMQMQSICIVIAYADG
                     KYMRMPHLCRCPAYGYVEPMLYSLFSRSDPKKASSGSAFRPPVRSGPETQDVAVLPAE
                     DDAICCVPPLPRDRPLTTGKRRFAYSLTVVHFRKLRKARKVGSGRNGRPVI"
     gene            5173..5391
                     /gene="ccdA"
                     /locus_tag="ISB30_004107"
     CDS             5173..5391
                     /gene="ccdA"
                     /locus_tag="ISB30_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAP1991680.1"
                     /translation="MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            5393..5698
                     /gene="ccdB"
                     /locus_tag="ISB30_004108"
     CDS             5393..5698
                     /gene="ccdB"
                     /locus_tag="ISB30_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAP1991681.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEVADLSHRENDIKNAINLMFWGI"
     gene            5867..6262
                     /locus_tag="ISB30_004109"
     CDS             5867..6262
                     /locus_tag="ISB30_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991682.1"
                     /translation="MNRTDGEPETDVMATAFSTMRKYGMNIITATQHMDINAYVKSDP
                     RMPRTRLRRYLGQYGLRGEALSAEVRRIVAGPERPDNTLKAGIFYTWPYGWEPADFTP
                     VMRRIFKGKRTRHVMLFDELPQLFGKNDY"
     gene            6289..6603
                     /locus_tag="ISB30_004110"
     CDS             6289..6603
                     /locus_tag="ISB30_004110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991683.1"
                     /translation="MGTPTFSSFNDVVRELEDVYGHQELWLYSGLNEDSPIETARRRQ
                     KWRSPKILKRNGRMVAEQSGQPDFWVLTGDYHLPQSEHSAPPWKACLINKVFKVYCSL
                     HC"
     gene            6614..7630
                     /locus_tag="ISB30_004111"
     CDS             6614..7630
                     /locus_tag="ISB30_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991684.1"
                     /translation="MTDCVTRTGDDKTMWLVTLPEIITNNSTRQEENLVVILSREESW
                     GPTSDHFPDGLYRVSCIMTLYLADTELTKTQTFTAIYWPQQKALHLFTDEFRLERRLQ
                     GLGLGSWITQQFVLWARGLPPATLVLPIEISRVDEENEENKIRRDRLWHAMGFRFPAG
                     ETSSMPLRADELQLPRGRCSTLRVEPLVSAVRRLEDCYRGLQEKIVQLEGKKRSQKQL
                     ITSLKNRPFYHLLHRKEGQLPDEKCDALPLRAEKLSLPQSGDSDLEVAQRATGVIRLA
                     TLCAQSQKRILELERELASQSEELVDLQAHPFFNLRDKYRGQILFWGWGMGFLCFIYW
                     MLVS"
     gene            7674..7819
                     /locus_tag="ISB30_004112"
                     /pseudo
     CDS             7674..7819
                     /locus_tag="ISB30_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7828..8622
                     /locus_tag="ISB30_004113"
     CDS             7828..8622
                     /locus_tag="ISB30_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HAP1991685.1"
                     /translation="MNNLVPVGSPASPGTLLPVAIDYPAALALRQMALVQDELPKYLL
                     APEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLVPPYPFVQLATLKQR
                     TEKAARTAGRAPAGSQVHRLVPLSDHHYVSQLQMMVATLKIPLERRNKRTGRMEKARI
                     WEITDRTVRTWLSEAVEAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFAMPEAEAVALIKKLTPNQST"
BASE COUNT         2137 a         2388 c         2429 g         2041 t
ORIGIN      
        1 aaaacgggag tgaccataga ttccgttttc tgaggtgccc ccataaagcc ctgcgcagcg
       61 cgcagggtca gcctgaatac gcgtttaatg accagcacag tcgtgatggc aaggtcagaa
      121 tagcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc ctgaatagct
      181 tcatcatcca gccagaaagt tatggagcca cggttgatga gggctttatt gtaggtgggc
      241 cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatac
      301 gtgatctgat ccttcaactc agcaaaagtt ggtaatgact ccaatttact gatagtgttt
      361 tatgttcaga tagtgcccga tgactttgtc atgcagctcc accgattttg agaacgacag
      421 ggacttcctg cccagccttg ccagatgctg tctcagattc aggttgtgtc gctcaattcg
      481 ctgcgtgtaa cgcttactga tgacgtgcag ttttcccttc agacgtgact catacatcgg
      541 ccagccgtcc gtcatccata ccaccacgtc aaaaactgac agcaggctca gaagacgctc
      601 cagtgtggcc atagtgcgtt caccgaaaac gtgggctacc accgccctgc gtatcctgtc
      661 atacgcgtaa aacagccagc gctgacgtga ttttgcaccg acgtagcccc attgctcgtc
      721 catctccgca cagacaatga catcactgcc cggctgtatc cgggaggtta ctgactgcgg
      781 cctgagtttt ttaaatggcg caaaatggtg ttgaggccca cgcccattag tcgtgcggtg
      841 gcacggcaac cgacgccgtt catagccata tcaatgattt tctggtgtgt accgggctga
      901 gaagcggcat aagtgaaggt gagctgccat gttttacggc agtgagagca gagataacgt
      961 tgatgcccgg cagtactttt gccattacgc atcacgcctt cagtagcgga acaggaggga
     1021 cagcagacgg agactgaagc cacgggagca cctcaaaaac accattatac actaaatcag
     1081 caagttggta gcatcaccat gttttcatac tggcgtgtat gccacattat tagcattttg
     1141 cgaataaaaa taactggcat aattgtattt aatgataatg ttacatattg ttagccaggt
     1201 tccggaaagc gagaaccgga accgcacctg tccagccggt attcccgtcg atgaccctcg
     1261 ctttttactg atttgtgccc gactgagaag tcggtaactc agtttcggaa tcggcgcaga
     1321 tgagtttcgg gaacaaaata aatgttgccg cgcgcccgtg agcatgccac gtacagcctg
     1381 ttacgggacg acggtggaag ggttgccagt tcctgacggg tcagaagctt ccagtgggag
     1441 gatcccatca caatgcagac atcctgaaag tgatcgagcc ctttactggc gccccagttc
     1501 atggaatagc agccgtaacg gtgatgttca cgatagaaga gcttaatcac cgtattatcg
     1561 gcgtgcaggg tggctgagcg ctctgtatct gcgatcgttt caatcaggga ggcatgtatc
     1621 ccatgggcag cgatacggat gttcagttgc ccggtgataa actcgcacac tgaagctgag
     1681 caacgccatg tccggttgag cgtgtcccgg tcaaccgcga aacctgctgc gtcgaagcgg
     1741 gcctcatatc gcgtgatgtc gtcgtgcagc gttgaattaa cgttgccgtc atggcttgta
     1801 tcaaaggtat gctgataaaa atcgccgcaa cagagcacag tgatttccgc gcggcagagc
     1861 tccagcagaa aattaaagtc atgtccggca aagtcctgga cttcatcgac aagcagttca
     1921 tcatagtaac gggcaagccg gatacggata tccggcagca acccccgggc tgtcagtaaa
     1981 tgtgcgagcc tccggtgata cagccgtccg gcgggatcct gataatgccg ggcattcgtg
     2041 cgcggtattc tgggtggggg ctggtcgaag ctcaggccac gcgatgccag ctgctcctgc
     2101 aggaaaggcc ggaagcaaaa accgtgcaga aactcaaacc aggtcatcac cctgatccct
     2161 tccgggataa agccaaaacg tctgataatc tgtgcccgca gatgcgcttc attattcacg
     2221 gtgaaggtca ggagcagcgt cctcctgtcc tcactcaacc gtcgtatcag cagcgtggtc
     2281 ttgcccgagc cggcaaccgc aaatatcact ctcttatcca tgccagtgcc tcctgaatat
     2341 aatcaggtac ggtcagctct ccggcgtgca gctgtaacag ccggaatgct gcttctgcct
     2401 tattggccag catgtattcc tgcaccgtga gtgtccggcg gggtccgcgg aaaagcgtat
     2461 cacacagatc agcgttatcc tgatacaggc tgatttcaaa tgtggatcgg gtgttatcgc
     2521 gatcagcaaa tacccgggag cgggaacaaa tcacgtcagc atagcgttca tcgcagtttt
     2581 gctgatagtt gccgtcatta tcacgaaggg ccgcgacgcg attttccaga agacgggcca
     2641 gttcaagata acgacggaaa ctcgttccgc cgatagcgat gatgtgaact ccgtcttcct
     2701 ccggtgccct gccatacagg cggcgataaa aggcctcgat cagaataaac tcagcatccc
     2761 cctccacgag cagcacacgt ctggccagcg caaattccag tacgttatta tccggtgctt
     2821 tcatgaagaa ggcggcagtc tcagcggaaa gttcattcat cagtaccggc cgggcagacc
     2881 cgaggaggat tgccttacgc agatcgaggc gggaggagat atggctgctg tgtgtggcaa
     2941 taaacacctg cgtctgccgt tcagtggcaa gctgattaac cagcctcttc atactgacat
     3001 ggctgagatg attttccggc tcctcaagca acagtacgtg gagctctccc tgctgctgat
     3061 gccgctgcag cgcaaactcc gttttgatga agcactgcct tccttttcca cggtgacgaa
     3121 tggaaatacc gtcctcggta atgtccagat tcgcctcaag ccctgattta gcaccggaac
     3181 gcacgccaaa ctgatacgtt tccagcgtgt cgtttatggc tgacagatgc cgggtgcaga
     3241 agtgcatttt ctgttgccgg taactgtttt caagccggta ccgatcggcc accggcacat
     3301 tgacgctgta aactgtgcgc gtatattcct gtgcggcgtg ctcgttatcg atgcgtgcac
     3361 tgtctaagag cagatggcgc agatagcgtc tgaagctggc gaaatgacca ccggagaagg
     3421 tgctgaaccg cacggaatag tattcatagg gaaaattatc gggatcctgc tgtagtacat
     3481 tgtgaatatc ctggccgtat tcctccgtca ttggtgcaat acgcattctg agaccatccg
     3541 cattgatacc cgccagattt tgcctgccgt tcaggtcagg atctccccca ttactcagga
     3601 aaacgtcagc tgtcagcacg ggtaactgat cggcgcggcg ctcaccttcc tgaaactgcc
     3661 ttactgctga ctgggacagg agcgattcca cacccaatgc ctcgacccgg tgccggctgt
     3721 cactcagcac aaggtcaagg gccagcagaa tcgtgctttt cccggactca ttgtccccga
     3781 ccagaatgtt acggtcatga gtgaagcgca ggtccagttc cggaaacttt ttaaagtttt
     3841 gcagcataag ccgcgtgatc agaggcatct tttattcctt tatataccgc tgactgaaag
     3901 agtgtccggc tgcgccagac cgcctgtttt gttttacagc gttatcttac ccagtcttcc
     3961 agcacaagac ccggtacccg ctcaaattcc cgcgtattat tcgtcaccag cacggcaccg
     4021 gcggcgatgg cgtgcccggc aatggccgtg tcgttcgggc cgatcggcgt cccggccatt
     4081 cgcagcgcca ccttaatctc cgtggtggcg tccaccgcag cgcgatccca gggcaggatg
     4141 gcatcgaggc gggcgcagaa cgcgtcgacc agctcgacgt ggcgcgggga ggccttcggg
     4201 ccggtggccc cgaagcgcat ttcagaataa gtgatggccg agacaacgat gcgctggccg
     4261 cgcagcaccg cctgctccag gcgcttcagg acggcttccg gctgctcgcg catgatgaac
     4321 gagcagatac aggtgtcgag catataggtc ttgttcacag gttaaatcgt ccttcgtcgc
     4381 tgacaacgtc ctcgcgctcc gccataaagt ccgggtctgc cttctcgaac tgcgcgaacg
     4441 agccccaggt cggccggacg ggacgcaaaa taatgctgtc cccttcccgg acgatctcca
     4501 gctcgctcac tccctcaaaa tccagatcgc ggggcagacg aatggcgcgg ttattgccgt
     4561 ttttaaaaat ggatacagtt ctcatcattt cctcctggcc gaccggcctg tatgactatg
     4621 ttcagtatat gcagatgcaa agcatatgta tagtcatagc atatgcagat ggtaagtata
     4681 tgcgtatgcc acacctatgc agatgcccgg catatggata tgttgaacct atgttgtatt
     4741 ctctcttttc ccggtcagat ccgaaaaaag ctagttccgg atctgctttc cggcctccgg
     4801 tgcgcagcgg cccggaaaca caagatgtag cggtgttacc agctgaggat gacgctatat
     4861 gttgtgtccc tcccctgccc cgcgaccggc cgctcaccac cggaaaacgc cgctttgctt
     4921 attctctcac cgtggtacac ttccggaaac ttcgtaaagc gcgaaaagtc ggatccggac
     4981 ggaacggccg tcctgtaatt taacggcgtg gctgtatggc tgcttgttat ctctaaggtg
     5041 attattttgt atgtgaattt cacgaagtga aattaatggc gacataacag tatgtttcag
     5101 gcgggcgtga gtcagctgca tccttacgct atactgatat gcatatcatc agtatgtatt
     5161 ccggagtgca ttatgaagca gcgcatcact gtcaccatag acagcgacag ttatcagtta
     5221 ctgaaatccg cagacgtcaa tatctccgcc ctggtgaaca gggccatgca gaatgaggcc
     5281 cgtcgtctgc gttccgaacg ctggcaggct gaaaatcagg aagggatggc cgaggttgcc
     5341 cgctttattg agatgaacgg ttcattcgcc gacgaaaaca gggactggtg agatgcagtt
     5401 taaggtttat gcctataaaa gagagagccg ctatcgtctg ttcgtggatg tgcagagtga
     5461 catcatcgat acccccgggc gacggatggt gatcccgctg gccagcgccc ggctgctgtc
     5521 ggataaggtt tcccgtgaac tctatccggt ggtgcacatc ggggatgaaa gctaccgcct
     5581 gatgaccacg gacatggcca gcgtcacgtc ctccgtcacc ggggaggaag tggccgatct
     5641 cagccaccgg gaaaatgaca tcaaaaatgc gataaacctg atgttctggg gaatttgaca
     5701 gcctccccgc cctgaaagac atcgaggatg tcaaaacgaa gcattgccgc agagcttaac
     5761 cctcagcatg aactgccctc cggagcggat gccctgcagg ctttcgcggg gtcagcttaa
     5821 acgaacggtt cacacaaaca cccttaagtc agtaaaagga aaccagatga acagaactga
     5881 tggcgaacca gagacagacg tgatggccac cgctttcagc accatgcgca agtacggcat
     5941 gaatatcatc accgccacgc agcatatgga tattaatgct tacgtgaaat ccgatccgcg
     6001 gatgccgaga acgcggctgc gtcgttacct gggacagtac ggcctgcgcg gtgaggccct
     6061 gagcgcggag gtaagacgta tcgtcgctgg ccctgaacga ccagataaca cgctgaaagc
     6121 cggaatcttc tatacgtggc cctatggctg ggagcctgcg gatttcacgc cagtgatgag
     6181 gcgcattttc aagggaaagc gcacccgcca tgtgatgttg tttgatgaac tgcctcagct
     6241 gtttggcaag aatgactatt gaaccggata ctatttttga ggcgaggtat gggtacacca
     6301 acatttagct catttaacga tgtggtccgg gagcttgagg atgtatatgg ccaccaggag
     6361 ctgtggcttt attctggcct taatgaggac agccccatcg agacagcccg caggcgtcag
     6421 aaatggcgat ctccaaaaat tctcaaacga aacggcagaa tggtggcaga acaatcaggc
     6481 cagcctgatt tctgggtgct cacgggtgac taccatctgc cgcagtctga acacagcgct
     6541 ccgccgtgga aagcctgcct catcaataag gtctttaaag tgtattgttc acttcactgt
     6601 tagggaaaaa aacatgaccg actgtgttac ccggactggt gacgacaaaa caatgtggct
     6661 cgtgacattg ccggagatca ttacaaataa cagtacccgc caggaggaaa atctggtcgt
     6721 tattctgagc agggaggaat cgtgggggcc aacttctgac cattttcccg acggattgta
     6781 ccgggtctcc tgcataatga cactgtacct tgccgatacc gaactgacga agactcagac
     6841 tttcactgca atctactggc ctcaacagaa ggccctgcat ctgtttacag atgagttcag
     6901 actggaacgc cgcctgcagg gactcggcct gggtagctgg atcacgcagc agtttgttct
     6961 ctgggccaga ggccttccgc cagcaaccct ggttttgccg attgaaattt cccgggttga
     7021 tgaagaaaac gaagaaaaca agatccggag ggacaggctg tggcacgcaa tgggtttccg
     7081 gttcccggcg ggggaaacca gctcaatgcc gctgcgggca gacgaactgc agttgccacg
     7141 gggcaggtgc tcaacgctcc gggtggagcc cctggtatcc gcggtacgcc ggctggaaga
     7201 ctgttatcgc gggctgcagg aaaaaatcgt gcagctcgaa ggcaaaaaac ggtcgcaaaa
     7261 acagttaatt acttcactta agaatcgtcc attttatcat ctgttgcaca ggaaagaggg
     7321 ccagctcccg gacgagaaat gtgacgccct gccgctgcgg gcagaaaaac tgtctctgcc
     7381 gcagagcggg gattcagacc tggaggtggc gcaacgggca accggggtca tccggctggc
     7441 taccctgtgt gctcagtcgc agaaaagaat cctggagctg gaaagggagc ttgcctcgca
     7501 aagtgaggag ctcgttgacc tgcaggcaca tccctttttt aacctgcggg ataaataccg
     7561 cggccagatc ctgttctggg gatgggggat gggttttctg tgctttatct actggatgtt
     7621 ggtgtcatga atgttcccgt tccatagcca cccactgaag aggagtgaaa cctgtgcccg
     7681 aaaatatcgt gtctaccgtc attgacgggg tagttttgtt tgtgacttcg tctgttttct
     7741 ggctggccgt cgctggctgg ttagtcatcc tgactccggt aatattacga agaaaattct
     7801 cggcaaagaa aaaaattaac agaaattatg aataacctgg ttccagttgg atctccggca
     7861 tctcccggaa cattgctccc ggtggccatc gattaccctg ccgccctcgc cctgcgccag
     7921 atggcgctcg tgcaggatga actgccgaaa tacctgctgg cgccggaagt cagcgccctg
     7981 ctccattacg tgccggatct tcaccgcaag atgctgctgg cgacgctgtg gaacaccggc
     8041 gcgcgcatta atgaagcgct cgcgctgacc cggggggatt tttcgctggt gccgccgtat
     8101 ccgttcgtgc agctggccac gctcaagcag cgcaccgaga aagcggccag gacggcaggt
     8161 cgtgcccccg caggcagtca ggtccatcgt ctggttcccc tttccgatca ccactatgtc
     8221 agccagctgc agatgatggt ggccaccctg aaaattccgc tggaaagacg aaacaagcgc
     8281 accggcagaa tggagaaggc acgtatctgg gagatcacag accgtaccgt gcgcacctgg
     8341 cttagcgaag cggtggaggc cgcagcggcc gacggggtga cgttctcagt accggttacc
     8401 ccccatacgt tccgtcactc ctacgcgatg catatgctgt acgccggcat acctctgaag
     8461 gttttgcaga gcctgatggg ccacaaatcg atcagttcca cggaggtcta tacgaaggtg
     8521 tttgcgctcg atgtcgcggc acggcaccgg gtgcagtttg ctatgccgga agctgaagct
     8581 gttgcgttga taaaaaagtt aaccccaaac caatctacat aggtcaacta tgggtaatac
     8641 cccaggtttc gacctaaagt agttcttgtg tgggaaacag tccaagctta atgggggctt
     8701 atccagcctc actttttaca gctttcaatc acattaatta tgttcagaaa ggccctcatt
     8761 gccctaaaga aaaaaaatgc agccatgaaa ataaggaata attgagatac aactcctgct
     8821 aaatcatggt tgctaccagc cactgatagc aacgtaaata gtaatgcaag cgaaaaatgt
     8881 aaggcgattg tagtatttga attcatgtat atccccttat gtataggact gaggccatcc
     8941 gcaaattttc actatcgcga agttgttgcc tcgatataaa aggtgttacc accct
//
LOCUS       DACADF010000171         8886 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.173, whole genome shotgun sequence.
ACCESSION   DACADF010000171 DACADF010000000
VERSION     DACADF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.173"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..661)
                     /gene="trkD"
                     /locus_tag="ISB30_004114"
     CDS             complement(<1..661)
                     /gene="trkD"
                     /locus_tag="ISB30_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Responsible for the low-affinity transport of
                     potassium into the cell; involved in potassium ion uptake
                     under hyper-osmotic stress at a low pH; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP1991686.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GA"
     gene            884..2380
                     /gene="ravA"
                     /locus_tag="ISB30_004115"
     CDS             884..2380
                     /gene="ravA"
                     /locus_tag="ISB30_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAP1991687.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            2374..3825
                     /gene="viaA"
                     /locus_tag="ISB30_004116"
     CDS             2374..3825
                     /gene="viaA"
                     /locus_tag="ISB30_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAP1991688.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTLVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3830..4822)
                     /gene="asnA"
                     /locus_tag="ISB30_004117"
     CDS             complement(3830..4822)
                     /gene="asnA"
                     /locus_tag="ISB30_004117"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAP1991689.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSKEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4974..5432
                     /gene="asnC"
                     /locus_tag="ISB30_004118"
     CDS             4974..5432
                     /gene="asnC"
                     /locus_tag="ISB30_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAP1991690.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            5522..5965
                     /gene="mioC"
                     /locus_tag="ISB30_004119"
     CDS             5522..5965
                     /gene="mioC"
                     /locus_tag="ISB30_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAP1991691.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKIEAELKNSGAKQTGETLKINILDHNIPEDPAEEWLGSWINLLK"
     gene            6344..8233
                     /gene="mnmG"
                     /locus_tag="ISB30_004120"
     CDS             6344..8233
                     /gene="mnmG"
                     /locus_tag="ISB30_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP1991692.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            8297..>8886
                     /gene="rsmG"
                     /locus_tag="ISB30_004121"
     CDS             8297..>8886
                     /gene="rsmG"
                     /locus_tag="ISB30_004121"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP1991693.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGER"
BASE COUNT         2257 a         2290 c         2256 g         2083 t
ORIGIN      
        1 ctgcccctaa tgcaataaaa gaaaccgttt tgtattcaag gaagaaatgc accgcccaca
       61 tcggattcag tgcatgcagc acttccgggt tagcaataat gctacgtaac cccagccctg
      121 ccagaatcaa aaaccaggtc agcatgatcg gcgcaaacag cttaccgacc atagctgtgc
      181 catgtttttg aatcatgaat aataatgtga gaacgataat tgagagggga actatccagg
      241 tatccagctg cggggcgacg atttccagac cttcaatggc ggacatcacc gaaatagcgg
      301 gagtaatgac gacttcacca tagaaaaagc tgccgccgat tagccccata atcaccagca
      361 ttgatgtggt tcgcgccgac gtattacgcc cggcaagcga catcaacgtc aggatccccc
      421 cttcaccggc gttatctgcc cgcatcacga aggtgagata tttaatggaa accacaaaga
      481 ttagcagcca gaagatcagc gataaaaagc caaacacggc atcgcgttca acgccaaaac
      541 caaactggcc ggacaaacat tcacgaagtg tatataacgg gctggtacca atatcgccgt
      601 agacaactcc aatcgccgcg agggtaatcg cgggcaatga ttgcttatta tcagtgctca
      661 tagactagtc tttcgttgaa atatgaaatg tgtgcttagt cccttggccc acaaaaaagc
      721 gcacagtatg cacgattaac ggcaaaatcg tactcctaaa tgcggccaca ttaacctggc
      781 tcaaagaaaa atacccctcc tttgagacta ttttagccac cagcaagaaa cgtctatact
      841 cgcaatttac gcagaacttt tgatgaaagg acgccacttc attatggctc accctcattt
      901 attagcggaa agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag
      961 ccacgccatc cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg
     1021 cccaccaggt attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc
     1081 ccgcgcgttt gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct
     1141 ttctattcag gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc
     1201 ggaagctgaa atcgtctttc tggatgagat ctggaaagcg ggtccggcaa ttcttaatac
     1261 cttgctcacc gccattaacg agcgccagtt ccgcaacggc gcactcgtag aaaaaatccc
     1321 gatgcgtctg ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc
     1381 gttatatgac cgcatgctga ttcgtctgtg gttagataaa gtgcaggata aagcgaattt
     1441 ccgctccatg ctgaccagtc aacaggatga aaatgacaat ccggttcctg cctccttgca
     1501 gatcacagat gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga
     1561 tcatgtattt gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc
     1621 ttatgtctcg gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt
     1681 tagcggtcgc agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta
     1741 tgacgcgcaa agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc
     1801 ctggcaacag caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact
     1861 ccagcagcaa caaagcgata aaacagcctt aacggtaatt cgtctgggcg gcattttcag
     1921 ccgtcgtcag cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct
     1981 gcaaaaaccg ttaaaattgc atgatatgga agtggttcat atctcctttg agcgtagcgc
     2041 gctggaacag tggctgagta aaggtggtga gattcgcggc aaactgaacg gtatcggctt
     2101 tgcccagaaa ctgaatctgg aagtggatag cgcccaacat cttgttgtac gcgatgtaag
     2161 tttacaaggc agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat
     2221 aaaacaacaa ctggaagagc ttgaaagcga ctggcgcaag caacacgctt tattcagcga
     2281 acagcaaaaa tgtctgttta tccctggcga ctggttaggt cgcattgaag ccagcctaca
     2341 ggatgtcggc gcacagattc gccaggcaca acaatgctaa cgctggatac gcttaatgtg
     2401 atgctggccg tcagcgaaga gggattgatc gaagagatga tcatcgcgct gctggcctca
     2461 ccgcagctgg cagtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat
     2521 gttccccgct ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa
     2581 ctcaccgaag aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt
     2641 gtgcagctac cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa
     2701 gcccgacagt tggttgatgc taacagcacg atcacttcag cgttacacac gctttttctc
     2761 cagcgttggc gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa
     2821 gaagaacgcg aacaactgtt gagtgaagtt caggaacgca tgacgctgag cggacaactt
     2881 gaaccgattc tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt
     2941 cagcttaaac gtggcgacta tcagttgatt gtgaaatacg gtgaatttct taacgaacag
     3001 ccggaactga aacgcctggc agaacagctg gggcgttccc gggaagccaa atcaataccg
     3061 cgcaacgatg cgcagatgga aaccttccgc accctggtgc gcgaaccggc gacggttcct
     3121 gagcaggttg atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg
     3181 gcgacactag ggataacaga actggagtat gagttttacc gtcggctggt ggaaaaacag
     3241 ttgctcacct atcgcctgca tggtgagtcg tggcgtgaaa aagtgatcga acgcccggtg
     3301 gtgcataaag attacgacga acagccgcgc ggaccgttta ttgtctgtgt ggatacttcc
     3361 ggctcaatgg gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc cttgatgcgc
     3421 attgctctcg ctgaaaaccg gcgctgctat attatgctat tttccaccga gatcgtccgt
     3481 tatgagcttt caggcccaca aggcatcgaa caagcaatcc gttttttaag tcagcagttt
     3541 cgtggcggca ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagccgg
     3601 gaatggtttg atgccgatgc ggtggtgatt tctgatttta tcgcccagcg gttgcctgac
     3661 gacgtgacga gtaaagtgaa agagctacag cgggtacatc agcatcgctt tcatgccgtg
     3721 gcgatgtctg cacatggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt
     3781 gataccggga tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag
     3841 aagggacgct ctcgcgaaca gctgccggcc atacaccaca ctgaacctgg ccgatatgtg
     3901 gcagttgcag cagcagcatg gtcaaacgag actgcccgat accgccgccg atggtctgcg
     3961 gcatttcacc gcgcagcagc gcctgatgcc actccagctc caggcgatct tcgtcaccgg
     4021 tcagcgccag ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct
     4081 caaacgcatc ttccagtacc gggttccaca ccagaatatc gccgttcaga cccgcatggc
     4141 ccagctctga cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac
     4201 catcactcaa cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg
     4261 cccgctcacg ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta
     4321 cgaagtggat ctgatccggc aggaacggtg ccaggccaaa ctctttgcta accgcagctt
     4381 cggttgcttt aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagaattgac
     4441 gctcgccgtc gcccattacg cgttcccagt cccactggtc tacatagacc gagtgcaacg
     4501 gggaaagacg gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg
     4561 cgctgaagtc gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt
     4621 cgaactgggc atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca
     4681 agttatcctg cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc
     4741 ccagacgttc ttccagttga cgagaaaagt gagatttcac gaagctaatt tgacgttgtt
     4801 tggcaatgta agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca
     4861 acaaaaaccg acacacatgc aataatcatt caataaaaag cctcacgtgc ttttgattca
     4921 ttaaaaaaga aggctaaaat agaatgaatc atcaatccgc ataagaaaat cctatggaaa
     4981 attatctgat cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca
     5041 ccgcttacgc cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag
     5101 tagagaaaat gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc
     5161 agcttggtta tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactatc
     5221 cttccgcgct ggcaaagctg gaaagcctcg atgaagtcac tgaagcctat tacacaaccg
     5281 gccactacag catctttata aaagtgatgt gccgttcgat cgatgctctc cagcatgtac
     5341 ttatcaacaa gatccaaaca attgatgaaa ttcagtccac cgagacactg atcgtcctgc
     5401 agaacccgat catgcgtacc attaagccct gatcggcttt tttaatccca tacttttcca
     5461 caggtagatc ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt
     5521 tatggcagat atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga
     5581 acacctggct gaaaagctgg aagaggcggg ttttaccacc gaaacgctgc acggtccgct
     5641 gttggaagat ttatctgcct cggggatctg gctggttatc agctcaaccc acggtgccgg
     5701 agatattccg gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct
     5761 ttctgcggtc cgctttggcg caatcggtat tggtagtcgt gaatatgaca ccttttgtgg
     5821 ggctatcgat aaaatcgagg cagaactcaa aaattccgga gcaaaacaga caggcgaaac
     5881 actgaagatc aacattcttg atcacaacat tccggaggat ccggcagaag aatggctggg
     5941 atcgtggatt aatttactca aataagtata cagatcgtgc gatctactgt ggataactct
     6001 gtcaggaagc ttggatcaaa cggtagttat ccaaagaaca acgattgttc agtttttgag
     6061 ttgtgtataa cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac
     6121 agttaatgat cctttccagg ttgttgatct taaaagccgg atccttgtta tccacagggc
     6181 agtgcgatcc taataagaga tcacaataga acagatctct aaataaatag atcttctttt
     6241 taatacccag gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg
     6301 ggcttcaatc cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc
     6361 ttttgacgtc atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggccgcggc
     6421 gcgtatgggt caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag
     6481 ctgcaacccg gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact
     6541 cggcggtctg atggcgaaag cgatcgatca ggcgggtatc cagtttagga tactaaacgc
     6601 aagcaaggga ccggcggttc gcgctacccg agctcaggca gatcgtgtgc tctaccgtca
     6661 ggcggtacgt acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga
     6721 agatcttatt gtcgaaaacg atcgcgtggt cggtgccgtt acccaaatgg gactgaagtt
     6781 ccgtgccaaa gctgtcgtgc tcaccgttgg gacgttcctc gacggtaaaa ttcatatcgg
     6841 tctggataat tacagcggtg gccgtgctgg tgatccgccg tccattccgc tttctcgccg
     6901 tttgcgtgaa ctgccgctgc gcgttggtcg tctgaaaacc gggacaccac cgcgtattga
     6961 tgctcgaacc atcgacttta gcgtactggc gcaacagcat ggcgataacc caatgccggt
     7021 attctcgttt atgggcaatg cgtcccagca tccccagcag gtgccgtgtt atatcactca
     7081 taccaacgag aaaacccatg atgtgatccg cagtaatctc gatcgtagcc caatgtacgc
     7141 aggggtgatc gaaggtgtcg gcccacgcta ctgcccgtcg attgaagaca aagtcatgcg
     7201 ctttgccgac agaaatcagc atcagatctt ccttgaaccg gaagggctga cctctaacga
     7261 aatttatccg aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg
     7321 ctccatgcag gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga
     7381 cttcttcgat ccacgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt
     7441 ctttgctggt cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct
     7501 ggctggtctt aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcgcgttc
     7561 tcaggcgtat ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta
     7621 tcgtatgttt acctcgcgcg cagaatatcg tctgatgctg cgcgaagata atgctgatct
     7681 gcgtttgact gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt
     7741 taacgagaaa cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac
     7801 cccgtcggcg gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga
     7861 agccagtggt gaagatctgc tacgtcgtcc tgaaatgact tatgaaaaat taaccacgct
     7921 gacaccgttt gcccctgcgt tgacagatga acaggctgcg gaacaggttg agattcaggt
     7981 taaatacgaa ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga
     8041 gaataccctg ctaccagcga cgctggatta ccgccaggta tccggtcttt ctaacgaagt
     8101 gatcgccaaa cttaacgatc acaaaccggc ctctattggc caggcttcgc gtatttctgg
     8161 cgtcacgcct gcggctatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg
     8221 tcgtagcgca taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca
     8281 agaacaggta atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc
     8341 gcttaccgat caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa
     8401 taaagcgtac aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct
     8461 cgatagcatt gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg
     8521 accgggactg cctggcattc cactctctat cgtgcgtcct gaagctcatt tcacgttgtt
     8581 ggatagcctt ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga
     8641 gaatattgag ccagtacaga gcagggtaga agagtttccg tcagagccgc catttgatgg
     8701 cgtaattagc cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc
     8761 tggtgagcaa ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt
     8821 gttgcccgaa gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg
     8881 cgaacg
//
LOCUS       DACADF010000172         8885 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.174, whole genome shotgun sequence.
ACCESSION   DACADF010000172 DACADF010000000
VERSION     DACADF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.174"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..807)
                     /gene="dapB"
                     /locus_tag="ISB30_004122"
     CDS             complement(<1..807)
                     /gene="dapB"
                     /locus_tag="ISB30_004122"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP1991694.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLD"
     gene            complement(968..1333)
                     /locus_tag="ISB30_004123"
     CDS             complement(968..1333)
                     /locus_tag="ISB30_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP1991695.1"
                     /translation="MTYGEAYLEGWKNIFNYEGVSNRFEFWSFMIGSGVICLLPLLCW
                     WLAVTINNDYGVFIFFALPASFILTLIFAVPAIALAVRRMHDIGYSGWWVTIVVLIPV
                     TGVILLILCCLPSKSQDQA"
     gene            complement(1356..2270)
                     /gene="rihC"
                     /locus_tag="ISB30_004124"
     CDS             complement(1356..2270)
                     /gene="rihC"
                     /locus_tag="ISB30_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAP1991696.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYNFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAL"
     gene            complement(2336..3286)
                     /gene="ispH"
                     /locus_tag="ISB30_004125"
                     /gene_synonym="lytB"
     CDS             complement(2336..3286)
                     /gene="ispH"
                     /locus_tag="ISB30_004125"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAP1991697.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(3288..3737)
                     /gene="fkpB"
                     /locus_tag="ISB30_004126"
     CDS             complement(3288..3737)
                     /gene="fkpB"
                     /locus_tag="ISB30_004126"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAP1991698.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(3845..4339)
                     /gene="lspA"
                     /locus_tag="ISB30_004127"
     CDS             complement(3845..4339)
                     /gene="lspA"
                     /locus_tag="ISB30_004127"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAP1991699.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILVVMMYRSKAAQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(4339..7155)
                     /gene="ileS"
                     /locus_tag="ISB30_004128"
     CDS             complement(4339..7155)
                     /gene="ileS"
                     /locus_tag="ISB30_004128"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP1991700.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(7198..8139)
                     /gene="ribF"
                     /locus_tag="ISB30_004129"
     CDS             complement(7198..8139)
                     /gene="ribF"
                     /locus_tag="ISB30_004129"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAP1991701.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(8147..8365)
                     /locus_tag="ISB30_004130"
     CDS             complement(8147..8365)
                     /locus_tag="ISB30_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAP1991702.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAVGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            8468..8731
                     /gene="rpsT"
                     /locus_tag="ISB30_004131"
     CDS             8468..8731
                     /gene="rpsT"
                     /locus_tag="ISB30_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP1991703.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         2176 a         2500 c         2162 g         2047 t
ORIGIN      
        1 atcaagcaca tctcgcatat caaaaagacc actttcctta ccactcaacc acaaagccga
       61 tcttaccgca ccgttagcaa atgtcatgcg gctggatgcc ttatgggtga tctccagacg
      121 ctcgccaata tcggcaaaca tcgcggtatg ttcgccaacg atgtcacctg cacgcacggt
      181 ggcaaaacca atggtgccag gcacacgttc accggtatgg ccttcacgac tgtagaccgc
      241 gcaatctttc agatctttat caagagcgtg ggcgatcgcc tctcccattg ccagtgcggt
      301 gcctgacggc gcatcaactt tatgtctatg atgtgcttca ataatttcga tatcggtgta
      361 gtcccccatc actttggctg ctttctccag cagtttaagc atgacgttaa cgccaacgct
      421 aaaattggcg gcaaagacaa tcgcaatatc ggcggcggcg tcacgaatcg cctgtttacc
      481 ggcttcgtca aaccccgtag tgccgatcac catccctttg ccatgctggc gacaaaaagc
      541 gagatggttc agcgtacctt ccggacgggt aaaatcgata aacacatcaa aatcatcttt
      601 tatcgcatca agactacttt gcacagtaac gcctgttttc ccggctccgg ccagttcgcc
      661 cgcatcgctg cccagtaaag aagatccttc gcgctccagc gccgcgccca gctgcacgcc
      721 ttctaatgcc agcgccgctt gaatcaactg gcggcccata cggcccccgg ctcccgcgat
      781 ggcaacgcgg atgtttgcat catgcatagc tattctcttt tgttaatttg catagaccgt
      841 tttcagagta accagcgcat cgctacgccg ccagccaaaa acgctgataa ttagaaatta
      901 atgataaaca gggagggata tcagtgaaag gcatgacgca gaaagtgatg cgtcataccc
      961 tctggcatta tgcctggtct tgtgactttg acggcaggca gcacagtata agcagaatta
     1021 cgcccgtaac agggatcaac acgacaatgg taacccacca ccctgaatat ccgatatcgt
     1081 gcatccttct gacggctaac gctatcgccg gtacggcaaa aatcaacgtt aaaataaatg
     1141 acgcgggcag cgcaaaaaaa atgaaaacac catagtcatt gttaattgtt accgctaacc
     1201 accaacataa aagcggaagc agacagataa cgccactacc aatcataaac gaccagaact
     1261 cgaagcgatt ggaaacacct tcataattaa agatgttttt ccacccttcc agatacgctt
     1321 ctccatacgt catagaacac ttccttgttt tattattaga gcgccagggc cagcacctca
     1381 gccacccact gctggaagcc tttcacatcc agatccagcg ccacctgtac attggctggt
     1441 ttgcccaggc aaccgtcgat atcaacaacc gtcgtgcctg aggtaaattc accctgagtt
     1501 tctaccgcga caaaacacgg tttgagtgtg aacaattccg ggcgcaccag ccaggcgata
     1561 gcgcaaagat cgtgcatacg tagtccactt tgcatactgc cgctacggta gtggctaaac
     1621 agggcgtgta gcatttttcc ggtgcgattc aactccggca aagtagcgag atagtcaggg
     1681 gttaatattg cctgattggt gacatccaga ccgcacatga cgatttcgat gccgctacgg
     1741 aagacacagg ctgccgcttc tggatcggca gcgatattaa actcggcgtt tggtgtgcag
     1801 ttgccgcgac cagcagaacc gcccatgatc accagacggc gaatatacgg cttacattcc
     1861 gggcattgtg aaagtaacag cgcaatattg gttaacggac cgatagccac cagggtaacg
     1921 ggctctggcg cacgcatcag ggcatcacga attgccagaa acgccggtat acccagtggc
     1981 tgacggttgt gttcaacaaa gttatagcca gccattcccg attcaccgtg tacagatgcc
     2041 gcatcgcgcg gcgcacgaac cagtggtacg gcagcgccct gggcaagcgg aatatccaca
     2101 ttccagaaat gcagcagttg cagggcgttg cgggtggttt tctctaccga aacattaccc
     2161 gcgactgtgg tcatcagttg cagatcgagt tcgggggtaa aaatcgcggc ggcaatggca
     2221 acggcatcgt caatgccggg gtctgtatcg aggaagatag gtaaatgcat gttttctcca
     2281 taaaaaatgc cggtaacaag accggcattt tcgcataact tagactacta atgacttaat
     2341 cgacttcacg aatatcgaca cgcagctctt tcggcacttc gaagacgata ttttcttcgc
     2401 ggccttccag cggaatggct tcgccaccgc caagctgctg caagcgtgcc accacattct
     2461 gcaccagaat atccggagcc gatgcgcccg cagtcacgcc gacgcattta acctctttca
     2521 cccactcttc ctggatatct ttcgcatcgt caatcaaaaa tgcgcgtttg cccatacgct
     2581 gggccagttc cgccagacgg ttggagttgg aggagttttt cgaaccaacc accagtacta
     2641 cttccgcctg ctctgccagg gcgcgtaccg cttcctgacg gttagtcgtg gcgtagcaga
     2701 tgtcatcttt gcgcggaccg acaattttcg ggaagcgttt acgcagcgcg tcgatcacat
     2761 cagacgtgtc atccaccgac agcgtggttt gggtcataaa ggagagcttc tcttcatttt
     2821 tgaccgtcag tttccacaca tcgtctggcg attcgaccag atacattccc ccttccgggt
     2881 tgctgtactg gcccatcgtc ccttccactt ccgggtgccc ggcgtgaccg atgagaatag
     2941 attcttcacc acgacggctg gcgcgggcga cttccatatg cactttggtc accagcggac
     3001 aggtggcatc aaacaccgtc aaatcgcggc ttttcgcttc gttacgtact gcctgagaaa
     3061 caccgtgtgc ggagaaaatc aggatcgcgc cgtccggtac ttcgctaatc tgctcaataa
     3121 agatagcccc acgctcgcgc aggctatcga ccacgtagcg gttatgcacc acttcgtgac
     3181 ggacatatat cggtgcgccg taaatggcca gcgcgttttc aacaatgctg atagcgcggt
     3241 ctaccccggc acaaaaacca cgcgggttgg ccaacaggat ctgcatgtta cgcctccagt
     3301 gccggatcga tttccagcac ttcaatatca aaatgaacgg tctgcccggc cagcggatgg
     3361 ttgaaatcaa cggtaatgga gtcgccgtta atttcgcgga tcacgccagg catctcactg
     3421 ccatccattg cggtaaaaag catgattgcg ccaatttctg gctcgcctgc atccataaat
     3481 tcacggcggg agaagtactg aatcaggtcc ggtgacggca cgccaaacgc cgcatctggc
     3541 tccagcgaga aggtggtttt atcgcccact ttcagcccca gcaggtgttg ctccagccct
     3601 tcagaaagag aagcatcacc caggcggaac agcgccggtt taccgttgtt gcgggtagac
     3661 tcagcggtgg tgccatcatc gagtttcagc gtgaagtgca ccaggacggc gctattgctc
     3721 tgtacagatt cagacatgca ggtttgctct tatattttga ggatttttgc cggatgcggc
     3781 ctacgatttc gcagcaatct gtaggccgga taagatgcgt cagcatcgca tccggcaggg
     3841 tttattattg ttttttcgct ttagaaggta aaaaaccttc cagcacaatc agtgccgcac
     3901 cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa gtgccagtcg ccgacgtaga
     3961 agtcgatcat atcgacaacg aagccgtgcc acaggcggtc gaacaggttg cccagcgcgc
     4021 cgccaataat cagcgcgtaa gcgatattgt ttagcttctg cgcggccttc gagcgataca
     4081 tcatcactac caggatcacg ctaataccaa tcgcaatacc ggcaaagaac caacgctgcc
     4141 agccgccgct atcggcaagg aaactaaacg ccgcgccata gttacgcgca taatgcagat
     4201 taagcgacgg gaacagcggg accgtatccc ccagagcaaa gttctggagg atcaggtatt
     4261 tgctgcccag atcgataatc agcacgacta ccaccagcca cagccagcgt agccctgttg
     4321 aacagatcga ttgactcatc aggcaaactt acgtttttca ccgtcaccgg cgacgttgct
     4381 gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg ccgacatcct gggtgtagtg
     4441 ccagcagcga gggcacttct caccttcggc tttactcaac gcgactttca gccctttgag
     4501 gacttcgctc tgctgggcat cagcaggtgc gtcgttatag tctgcaacgg tagcgccgga
     4561 ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc agtttcgccg ccagttccgg
     4621 ttctgcatac aaggttactg cagcttccag cgagccgccc actttcttat cggcacgcgc
     4681 ttgctcaatg actttgttca cttcgccacg cactttcaac agctcgtccc agaacgcatc
     4741 gttcattgct tcactgtctg ccagaccaaa caggccttcg taccactcgc cggtgaagac
     4801 gtatttttca cgttcgcccg gcaggtagcc ccacacttca tcagcggtga aggagaggat
     4861 tggtgccatc cagcgaacca gcgcttctgc gatgtgatac agcgcagtct ggcagctacg
     4921 acgcgccacg ctgtccgctt tggcggtata ctgacggtct ttgatgatgt cgaggtagaa
     4981 ggaacccatc tcaacggagc agaagcgcat cagacgctgt accacttcgt ggaaatcgta
     5041 tgcttcgtac gccttgagga tgtcttcctg tgccgctttc gcacaaccta cggcccagcg
     5101 atccagtacc accatctctt ccggtttcac catatctttt gccggatcaa aaccgttcag
     5161 gtttgccagc aggaagcgcg cggtgttacg gatacgacga tagctgtcgg cagcacgttt
     5221 cagaatctcg tcagaaacag ccatttcgcc ggtgtagtcg gttgatgcca cccacagacg
     5281 cagaatatcc gcacccagtt tgttcatcac atcctgcggc gaaacggtgt taccgatgga
     5341 tttagacatc ttgcgaccct gaccatccac ggtaaagccg tgagtcagta cctgacgata
     5401 tggcgctttg cccttcatcg cggtagagat catcagggaa gacatgaacc agccacggtg
     5461 ttggtcagaa ccttccagat acatgtccgc tgcgtgaccg gcaaattccg gacgcacatc
     5521 aacaacggaa gagtgggtag atccggagtc aaaccataca tccagcgtat ccggtacttt
     5581 cacatactgg tcagcttcgt cgccgaggat ctctttcgca tcgagatccc accacgcctg
     5641 gatgccgtca acttcaacgc gttttgccac ttcttccatc agttcgagag tacgcggatg
     5701 cagttcttct gtgtctttgt gcacgaacag tgacatcggc acgccccagg tacgctgacg
     5761 agagatacac cagtcaggac ggttagcaac catcgactcg atacgcgcct ggccccagtc
     5821 agggatccac tgcacgcctt tgatctcttt cagtgactgc gcacgcagac ctttctgatc
     5881 catgctgacg aaccactgcg gcgtcgcgcg gaagatgatc ggcgttttat gacgccagca
     5941 gcacggatag ctgtgttgca ttttctcaac gtgcaacagt gcgccttttt cctgcaacaa
     6001 cgcaatgaca atatcgttcg ctttgaagac gttaacgcca tccagagtcg ggtaagtacc
     6061 cggcagataa gtgccgtccg ggccaaccgg gttagcggtt tccagaccat atttttgacc
     6121 gatcacatag tcgtccggac cgtggcctgg cgcggtatga acggcaccgg taccggcatc
     6181 cagcgtaacg tggtcgccaa gaattgccgg aacatcgaag tccataaacg gatgggtaaa
     6241 gcgcaacagt tccagctcag cacctttcac cgtgccaaga atggtgtaat cgctaacgcc
     6301 gatacgctgc attacgcttt caaccagatc tttcgccaga atcacggcct gaccgtcgat
     6361 ttgcaccagc gcataatcaa aatcaggtgc aatggagatt gcgcggttag caggcagcgt
     6421 ccacggcgtg gtggtccata taaccagcga aattgggccg ttaacattgc ttacgccaaa
     6481 tttcgttttc agcgcatcct gatcgaccgc ctggaaagcg acgtcgatgg acggagaagt
     6541 tttgtcgtaa tactcaactt ccgcttctgc cagtgcagaa cggcagtcaa cgcaccagtg
     6601 caccggcttc gcgcctttgt gcaggtgacc gttgccgatg attttgccca gcgcgcggat
     6661 gatgttggct tcagttttga agtccatggt caggtacggg tgcgaccagt cgcccagcac
     6721 gcccagacgg ataaagtctt tgcgctgacc gtcaacctgg gtcgcagcgt attcgcggca
     6781 cttggcgcgg aactcagcgg cggtgaattt ctcccccggc ttaccgtatt cttgctctac
     6841 tttcagttcg attggcagac catgacagtc ccagccaggc acatacggcg agtcatatcc
     6901 agaaagccct ttggacttaa tgataatgtc tttcagaatc ttgttaaccg agtgaccaat
     6961 atgaatgctg ccattcgcat aaggagggcc atcatgcaga atgaaggttt ttttgccttt
     7021 tttagctgca cgaatgatgc cgtacagatc atcatcagtc caacgcgcca gcattcccgg
     7081 ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt tccggcaaat tcagggttga
     7141 tttatagtca ctcatcagat tctcggttcc gtatttcggt ttgattacat aacaggctta
     7201 agccggtttt gttagcccaa aaaattcgcg ggcggttaat tcatcacgcg caatctgcgc
     7261 tttcagttcg tccagcgatg caaatcgctg ctcattgcgt atttttttac gcagcactac
     7321 ttgtatatgg cgaccataaa ggtccattgc aacatctaac aaatgcactt ccagttgctg
     7381 gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgca acgccgggta acggcttttc
     7441 gccaaggccc aacacttcta ccgcataaac ccctttcacc ggggaaacct gacggcgtag
     7501 cggtacattc gccgtcggga aacctatagt gcgccctaat tcatcaccgt ggactacacg
     7561 cccggagatg gcaaacgggt gccccagtaa actttctgcc agagccagat tgtcatccgc
     7621 cagcgcctga cgcacggcgg tgctgctgat acgcacgcca ccttcgcaaa aagtttgcgt
     7681 gctggtgata tcgaagccgt attccatacc cgctttctgt aataacaaga aatcgccttc
     7741 acgaccagcg ccaaagcgga aatcgtcacc tacggcaaga aattttaccc gcaagtgctt
     7801 caccagaaga tcactgatga agttttgcgc ggttaacgcc gcaaaacgcc tgtcaaaacg
     7861 cacgcacagc acgtaatcaa cgccacactc tgcaagataa cgcagttttt cccgcagccg
     7921 ggtcagccgt gccggggctt tatcagtagc aaacagttcc agtggttgag gttcaaaaag
     7981 catcaccatc accggtaagt tgcgcttgcg cccttcttcc tgcaagccct gtaacagcgc
     8041 gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc agcacacacc cttcttgcgg
     8101 ggcctggctg agattatgta tgccgcgtat cagcttcatg tctggctcaa aacagtgaaa
     8161 atcgtccgag tataccttgt acagcggtca aggttaaccg gcgattgagt accgaaatca
     8221 gaaagaagtg gcttttcatt gctatggcgc aaatcatggg aagaaactga ccgcctactg
     8281 caatttttct cgcggaaaag ctgtattcac accccgcaag ctggtagaat cctgcgccat
     8341 cactacgtaa cgagtgccgg cacattaacg gcgcttattt gcacaaatcc attgacaaaa
     8401 gaaggctaaa agggcatatt cctcggcctt tgaattgtcc atatagaaca catttgggag
     8461 ttggaccttg gctaatatca aatcagctaa gaagcgcgcc attcagtctg aaaaggctcg
     8521 taagcacaac gcaagccgtc gctctatgat gcgtactttc atcaagaaag tatacgcagc
     8581 tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt aacgaaatgc aaccaatcgt
     8641 ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa gctgcacgtc ataaggctaa
     8701 cctgactgca cagatcaaca aactggctta attgcctgtt gttgtcagct ttgtaaaaaa
     8761 acccgctctg gcgggttttt ttatgcctgc ggaagttacc gttaatcgat tatatatttt
     8821 ctccatgaat acatcataaa tataattgat gctttttcat tgtgaaagat aaaatatttc
     8881 atttg
//
LOCUS       DACADF010000173         8819 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.175, whole genome shotgun sequence.
ACCESSION   DACADF010000173 DACADF010000000
VERSION     DACADF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.175"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            120..623
                     /locus_tag="ISB30_004132"
     CDS             120..623
                     /locus_tag="ISB30_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAP1991704.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(701..952)
                     /locus_tag="ISB30_004133"
     CDS             complement(701..952)
                     /locus_tag="ISB30_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAP1991705.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1067..1153)
                     /gene="azuC"
                     /locus_tag="ISB30_004134"
     CDS             complement(1067..1153)
                     /gene="azuC"
                     /locus_tag="ISB30_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAP1991706.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1417..1740
                     /locus_tag="ISB30_004135"
     CDS             1417..1740
                     /locus_tag="ISB30_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991707.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1912..2409
                     /gene="ftnA"
                     /locus_tag="ISB30_004136"
     CDS             1912..2409
                     /gene="ftnA"
                     /locus_tag="ISB30_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAP1991708.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2447..2686)
                     /locus_tag="ISB30_004137"
     CDS             complement(2447..2686)
                     /locus_tag="ISB30_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAP1991709.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2877..4088
                     /gene="tyrP"
                     /locus_tag="ISB30_004138"
     CDS             2877..4088
                     /gene="tyrP"
                     /locus_tag="ISB30_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAP1991710.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4139..4804)
                     /gene="yecA"
                     /locus_tag="ISB30_004139"
     CDS             complement(4139..4804)
                     /gene="yecA"
                     /locus_tag="ISB30_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAP1991711.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5000..5086)
                     /locus_tag="ISB30_004140"
     tRNA            complement(5000..5086)
                     /locus_tag="ISB30_004140"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5050..5052),aa:Leu,seq:taa)
     gene            complement(5099..5172)
                     /locus_tag="ISB30_004141"
     tRNA            complement(5099..5172)
                     /locus_tag="ISB30_004141"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5138..5140),aa:Cys,seq:gca)
     gene            complement(5226..5301)
                     /locus_tag="ISB30_004142"
     tRNA            complement(5226..5301)
                     /locus_tag="ISB30_004142"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5266..5268),aa:Gly,seq:gcc)
     gene            complement(5453..6001)
                     /gene="pgsA"
                     /locus_tag="ISB30_004143"
     CDS             complement(5453..6001)
                     /gene="pgsA"
                     /locus_tag="ISB30_004143"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP1991712.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6058..7890)
                     /gene="uvrC"
                     /locus_tag="ISB30_004144"
     CDS             complement(6058..7890)
                     /gene="uvrC"
                     /locus_tag="ISB30_004144"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP1991713.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7887..8543)
                     /gene="uvrY"
                     /locus_tag="ISB30_004145"
     CDS             complement(7887..8543)
                     /gene="uvrY"
                     /locus_tag="ISB30_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAP1991714.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         2178 a         2240 c         2046 g         2355 t
ORIGIN      
        1 tcagaattga ctccacgatc acatttcgga ccggcagaaa ggaattattc tgcaaacagt
       61 aattatggtg ttttgattta tcttgcacct ctccacttct ggatataagg atattaggta
      121 tggcaaccgc tggaatgctt ctcaaactca actctcaaat gaaccgcgag ttttacgcat
      181 ccaatctcta ccttcacctg agtaactggt gttctgaaca gagtctgaac ggcaccgcca
      241 ctttccttcg cgcccaggca cagagtaatg tgacccaaat gatgcgcatg tttaacttta
      301 tgaagagtgt cggcgctacc cccatcgtta aagccattga tgttcccggt gaaaaactga
      361 actctctgga agaactgttc caaaaaacga tggaagaata cgagcaacgt tcaagcacgc
      421 tggcacagtt agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc
      481 gcgatctgga aaaagaacag cagcatgatg gcctgttact gcaaaccatt cttgatgaag
      541 tgcgcagtgc gaaacttgct ggtatgtgtc ctgtgcagac cgaccaacat gttctgaatg
      601 tcgtgtcgca tcagctacat tgatcatcat cggcgctaat gcattgcgcc gatgaagttt
      661 ttgagaaacc gctgcctcat ttttttgaag cagcggtttt ttaatgggat tcgccctgtg
      721 gggtaaactt gagttcaatc aacgcgatgg ctttttggat tgcccgcatg gtgaccgggt
      781 ctgcggcggc gggatggtta gtaaagtcga tattcttcag ctggctggac attttttcgc
      841 gaacttcaac gggcgcgatc acatcgagaa catccagaat ttgtttgata accaactggc
      901 aagcaaccac atcagaaacc agttcctgat cggcattcag cggctgggac atcgtaaact
      961 cctgatagca ttttgaaagc cgttatagta gcggcttcac atcttcagcg atagtcacat
     1021 ccaccgtcat cagaacacaa aaaaacctgc cggagcaggt tttttgttat cggaacatat
     1081 tgcctggcgg tacgtctttg aacgtcttgc aatagttatt gaacatactt ttcaggatct
     1141 tgcgcagttt catcgcggta ctccgaccat ttgttataca ggtgttattg tctttgcgtt
     1201 tataatatga caaccatcac aaaaatcaat ctttatgtga tgtaaatcac ataaataccc
     1261 ctttaatgtt ataaaaatga taatcaaaaa agcagccctc caatttctga cacatacaga
     1321 tggcaagaaa tagcgcctgc caggcgtctt ttccggccat tgtcgcagcg ctgtaacgcg
     1381 taaaatactg ctttctttta ctcttctggc tggaccatga gacttctgat tctgactctt
     1441 tccttaataa cgctgacggg gtgtacggtt actcgccagg cccatgtgag cgaagttgac
     1501 gcggcaacag gtattgttcg gttggtttat gatcaggctt ttttgcagca tgcccgtacc
     1561 gatcgttatg tcagtcgcgg gatcgccgat cgcgcatgcc agcaggcagg ctatacccac
     1621 gctatcccct ttggtcagcc agtaggcaac tgtagcctct ttgcgggttc cttatgtttg
     1681 aatacggaat tcactttgtc atatcagtgc catcattccg ccttccctgt ctttctctaa
     1741 acgttttaat cagcgagggg gatcttcgct gattaaataa atagcggtaa tgcgttttat
     1801 tcccattcgt atttttaata aatgatgttt atattttacc ttttgcaaat aataaaataa
     1861 caaattatag tgacgctaca gcaacaaata taacctttgt ggagcactat catgctgaaa
     1921 ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt
     1981 tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg
     2041 cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat
     2101 accggcaact taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt
     2161 gatgaattat tccaggaaac ctataaacac gaacaattaa tcacgcagaa aattaacgaa
     2221 ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct gcaatggtat
     2281 gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg
     2341 gcaggcaaaa gtggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca
     2401 caaaactaat gctctccggc ggcaggtttt ctgccgccgt ttcaaatcag tgacggcaaa
     2461 tcttactctg atcggttgaa aaaccttctt ctgaaggtat aaatttgcct tttgctgcca
     2521 gaaacgccac caactctccc gcagtcatcc cttctgccga gcaggtgtga aaacgtgcct
     2581 gttcaccaaa acgcgcttta attgctgctt ccagactggc atgcgtatat tgctcgcctg
     2641 attcaatcat catatttaac acttcatgac cgtgaataga gtccatcgtt cctcctcaaa
     2701 aaaagcctag cgtagcgatt gctgcttatg aagactttgc gccagcgcag aactgaacgc
     2761 tttttattgt acatttatat ttacactata tgtaacgtcg atttgacgaa tcagccgtta
     2821 tgccttaacc tgcgccgcag atatcactca taaagatcgt caggacagaa gaaagcgtga
     2881 aaaacagaac tctgggaagt gtttttatcg tggcgggaac cacaattggc gcaggcatgc
     2941 tggcaatgcc gctggctgcg gcaggtgttg gttttagcgt cacgttaatc ttgttgattg
     3001 ggctttgggc gttgatgtgc tacacggcgc tattactgct ggaggtgtat cagcatgtcc
     3061 cggcagatac cggtctgggc acgctggcaa aacgctatct gggacgctac ggtcagtggc
     3121 tgacgggctt cagtatgatg ttcttaatgt atgctctgac tgcggcatac atcagcggtg
     3181 ccggtgaatt gttggcctcc agcatcagcg actggacagg tatttcaatg tcggcaaccg
     3241 ctggcgtgct gttgttcact tttgttgccg gtggcgtggt ttgtgtcgga acttcgctgg
     3301 tcgatttatt taaccgtttt ctgttcagcg ccaaaattat ttttctggtg gtgatgctgg
     3361 tattgctgct accgcatatt cacaaagtga atcttttaac cctgccgttg caacagggac
     3421 tggcactgtc tgcaatcccg gtgattttta cgtcgtttgg ttttcacggt agcgtgccga
     3481 gtattgtcag ctatatggat ggcaacattc gtaagctacg ctgggtgttt ataatcggta
     3541 gtgcgatccc cctggtggca tatattttct ggcagctggc gacccttggc agcattgatt
     3601 caacaacctt tatgggattg ctggctaatc atgccggatt aaacgggctg ttacaggcgt
     3661 tacgcgaaat ggtggcctct ccgcatgttg agctggcagt gcatttattt gctgatttag
     3721 ccctcgccac gtccttcctc ggcgtagcgt taggcttatt tgattatctg gcagatttgt
     3781 ttcagcgttc aaataccgtt ggtggacgat tgcaaactgg tgcaattacc tttctgccgc
     3841 cactggcgtt tgcactgttt tatccacgag gatttgtgat ggcgctgggt tacgccggtg
     3901 tggcactggc ggtactggca ttgattatcc cttcgctgtt gacctggcaa agcaggaagc
     3961 acaatcctca ggcgggttac cgggtgaaag gtggtcgtcc ggcgctggtg gtggtgtttc
     4021 tctgtggtat tgctgtgatt ggcgtgcaat ttttgattgc ggcaggcttg ttaccagaag
     4081 tggggtgatt agattcttat tgggtgccag aattaacgct gacacccaat ttgacctctt
     4141 aatgcaggca gcactgctta aatttcttac cactaccgca cgggcaagga tcattacgcc
     4201 ccggtttctc ttctgctttg atcggttgct gaacagcttt ttcctgcgga tgcgccatcc
     4261 agtacgcatg tagatcaagc gccgccagtc gaatggcatc tacgctctct tcaaacgctt
     4321 ctggcgacat cttctctacc cgctcgaagt tttcctcagt accgtgcagc gcaatcgcct
     4381 ccagcgctgg ttttaacgaa tcgggcaacg ttgaccagtc agaaagtgcc acgccccgca
     4441 tatagccaaa gcaccactcc tcaacaatcg tcagctcgct gccatcgact tctcgcaagc
     4501 cgaataatgg ctcaaactgc tccggaaatt cgttcagacg ctctgcggta tcggccatat
     4561 gttgaaaagc caggttcata aagcgcgtca tctctttctc tgacgcccag cgcggcacat
     4621 agtcagcccc accccacacg gcaaccagcc actgtgccgg ttcaatctct tgcggagaac
     4681 tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc aagaatggcg tggtcagtgt
     4741 tgtatttggt cagaatatcg tccagccatt ccaactcact ttcgtttaac ggtcccgttt
     4801 tcatacgctt ttccttgtgg atctcaactc gccagcacct atcttacatg ccggtccgta
     4861 tcagagatac tttttgagtg gctttgccgg tgattaaaaa ttaaggaggg tgtaacgaca
     4921 agttgcaggc acaaaaaaac cacccgaagg tggtttcacg acactgctta ttgctttgat
     4981 tttattctta tctttcccat ggtacccgga gcgggacttg aacccgcaca gcgcgaacgc
     5041 cgagggattt taaatccctt gtgtctaccg attccaccat ccgggctcgg gaaaaaagtg
     5101 gaggcgcgtt ccggagtcga accggactag acggatttgc aatccgctac ataaccgctt
     5161 tgttaacgcg ccaaattctt caggcctttc agccagacat ccgctgacgc cgatgccttt
     5221 taaactggag cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc
     5281 taccaactga gctattcccg cattcatcaa gcaatcagtt aatcacttga ttttattatc
     5341 gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta cttacctggc gcgatgagtc
     5401 aacgatattt ttcaccactt ttgatcgttt gctgaaaatt gcgccgaaac gatcactgat
     5461 caagcaaatc tgcacgcgca gcgctcaaat attgcaacat tgaccacaga gtcagtaccg
     5521 cagccacaaa gaaaagtgca ataccggcgt actcaaccca aatattcgga cgccacagca
     5581 gccatgccaa cgccaccatc tgggcagtgg ttttcacttt tccaatccag gagacagcca
     5641 cgctactgcg tttacccaac tccgccatcc attcgcgtag cgcagaaata ataatttcac
     5701 gggcgatcat cgttgctgcc ggtaaggtca cccaccagct gtggtaatgc tcggttacca
     5761 gcaccatggc gatagccacg agaactttat ctgcgacagg gtcaaggaaa gcaccaaacc
     5821 gggtactctg attccagcgg cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga
     5881 cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac aggcaaataa aagaccaata
     5941 caaagaatgg gataaggatg acacggaaca gtgtaagcaa cgtagggata ttaaattgca
     6001 taatgacggg taactatctg ttgtcagtaa gattacccct atgttgctac agagacatca
     6061 atgtttcaac gaccagaaga tcttttctgc cagaccttgc gaaatacccg gcacttttgc
     6121 aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt tcaacaacat
     6181 ttgccgacgt tttggcccga cgccttcaat ggtttccagg gaactggtat ttttgacctt
     6241 cgcccgtttt ttacggtgcc cgccaatcgc gtgatcgtgt gattcatcgc gaatatgctg
     6301 gataacatgc agcgcgggag aatctggcgg caaactaaat ccctcacctt ccggctcaaa
     6361 gaacagcgtt tccagcccag ccttacgatc tgctcctttg gcaacgccaa gtagcagcgg
     6421 atgattttta tcccatgaga catccagttc ggcgaaaaca tttttcgcct gcgcaagctg
     6481 gcctttgccg ccgtcaataa ggatcacatc cgggatctta ctgtcgtcga tagctttacc
     6541 ataacgccga cgcagcacct gattcatcgc cgcgtaatca tcgcccggcg tgatgccagt
     6601 aatgttatag cgccgatact ccgcacgcag cgggccgttg gcatcaaaca ccacacagga
     6661 agcgacggtt tgttcaccca tggtatggct gatgtcaaag cactccatcc gcttcacttc
     6721 cggcaatttc aacacgctgg caagcgcggt cagccgctgg tgaacggtag attgctgcga
     6781 aagtttgctg gttaaggccg tcgccgcatt ggtacgcgcg agtttcagat aacgcgctct
     6841 atcgccacga ggtttggttt gaacattaat cttgcgtccc gccagttctg aaagggaatc
     6901 ggcgagcagc gttttatcgc taagattaaa atcgagcagg atctcacccg gtaaggtgcg
     6961 catctggctg ccttgtaaat agaactgacc tacgaaggtt tccaccacct cgctcagttc
     7021 cgtaccgcca ggcactttcg ggaaatagct gcggctgccg agcactttgc cctgacgaat
     7081 gaacaatacg tggacacaag ccatacccgc atcgaacgcc actccaataa cgtcaaggtc
     7141 gtcgccggta ttggaaacaa actgtttttc ggtgacacgt cgcaccgcct gaatttggtc
     7201 gcgaatccgc gccgcttctt caaactccag attctggctg gcagtttcca tacgactaat
     7261 gagttgcgta agcacctgat catctttgcc agacaaaaac aggcgcacat actcgacctg
     7321 ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac ggccccagac agcgccctat
     7381 ctgatattgc agacacggac gcgagcggtt gcgataaaca ctgttttcgc actggcgaat
     7441 ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg
     7501 gccgaaatat tcacctttgg catgcttcgc tccacgatgc atcgccagac gcgggtgggt
     7561 atcgccactc aggaagataa aaggatatga tttatcatcg cgtagcaaaa cgttgtaacg
     7621 cggctgataa agtttgatgt agttgtgttc cagtaacagc gcttcggttt ctgtatgagt
     7681 aaccgttaca tcaatttgct ggatctgggc gaccagcgct tcggttttgc gcgaagcgag
     7741 gttgctacgg aaatagctgg aaagccgttt tttcaggtct ttcgctttgc cgacatagat
     7801 aaccgtacca ccagcatcat acatgcgata aacgcctggc tggctggtta cggtttttaa
     7861 aaacgctttt gcgtcaaact gatcactcac tgacttgata atgtctccgc attacacaga
     7921 ccatggcgaa ttgccaggtg agtcagctca acatcgccat gaatgtttag tttactgaac
     7981 atacgatagc ggtagctgtt caccgttttc ggactgagat tgagctgttc tgagatctca
     8041 ttgaccttct ggcccttggt gatcatcagc ataatctgca attcacgttc agacaaactg
     8101 gcaaatgggc tttctgtttt ttctggttcg atctggctta acgccatttg ttgagcgata
     8161 tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa tcgcactcac gacttcctgc
     8221 ggagccgcgc ctttgctgag gtagcccgca gctccggcct gcatgacttt cgctggtaaa
     8281 gggttttctg tatggacggt aagcatgatg attttgacat cagctgtgga acgcgcgatt
     8341 ttacgcgtcg cctcaagacc gccaatgccc ggcatactca tgtccattag caccacgtca
     8401 acggcatttg cccggcacca tttaacggcg tcttcaccgc acgatgcctc accgacgact
     8461 tttataccct ttatatcttc cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca
     8521 tcaacaagta gaacgttgat caaaggaata tctccagaaa tagggataac gctactgata
     8581 gttagtcatt cgtatattag cggtttttat tgcaactttg aaacgttaaa aatggtgggt
     8641 attcgatttt tctctcgttt ttgtcaattc gattgtccac agttatggtc acgcccgtca
     8701 ccaacggctt ccgggatcgt ttcacagtaa gcccggcgat gaaatgtgac aaaaattgac
     8761 tttattcagc aaaaatgaaa atcagcccgc tgaatatgta acattaatta acccgaaca
//
LOCUS       DACADF010000174         8761 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.176, whole genome shotgun sequence.
ACCESSION   DACADF010000174 DACADF010000000
VERSION     DACADF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.176"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            104..1153
                     /gene="allD"
                     /locus_tag="ISB30_004146"
     CDS             104..1153
                     /gene="allD"
                     /locus_tag="ISB30_004146"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HAP1991715.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1175..2410
                     /gene="allC"
                     /locus_tag="ISB30_004147"
     CDS             1175..2410
                     /gene="allC"
                     /locus_tag="ISB30_004147"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAP1991716.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2421..3206
                     /gene="allE"
                     /locus_tag="ISB30_004148"
     CDS             2421..3206
                     /gene="allE"
                     /locus_tag="ISB30_004148"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAP1991717.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFVN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVYGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3335..4480)
                     /gene="glxK"
                     /locus_tag="ISB30_004149"
     CDS             complement(3335..4480)
                     /gene="glxK"
                     /locus_tag="ISB30_004149"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HAP1991718.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTIFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGITGDGKTAVIEMAAASGLMLVVPE
                     KRNPLLASSFGTGELILHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQP
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4502..5803)
                     /locus_tag="ISB30_004150"
     CDS             complement(4502..5803)
                     /locus_tag="ISB30_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAP1991719.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IAAPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYSRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(5859..7220)
                     /gene="allB"
                     /locus_tag="ISB30_004151"
     CDS             complement(5859..7220)
                     /gene="allB"
                     /locus_tag="ISB30_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HAP1991720.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKDGKIAAIGQDLGDAKDVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(7280..8734)
                     /locus_tag="ISB30_004152"
     CDS             complement(7280..8734)
                     /locus_tag="ISB30_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HAP1991721.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
BASE COUNT         2349 a         2308 c         2097 g         2007 t
ORIGIN      
        1 attctaacta tatttttttg cctgtctgga tcacataatc cagatatttt ccctgtatgt
       61 taattgcagt catgcttcac accgtcgtta aaaaggaaga cagatgaaaa tcagtcggga
      121 aacactccac cagctaatcg agaataaact ctgccaggct gggttaaaac gtgagcacgc
      181 tgcaaccgtg gctgaagtat tggtttacgc cgatgccaga gggatccact ctcatggcgc
      241 ggtgcgcgtt gaatactacg ccgaacgcat ttcaaaaggc ggcaccaacc gcgaaccgga
      301 gtttcgtctt gaggaaaccg gaccgtgctc ggcaatttta catgccgaca atgccgccgg
      361 acaggtcgcg gcgaaaatgg gtatggaaca tgccatcaaa accgcccagc aaaaaggcgt
      421 tgcggtggtc ggtatcagcc ggatgggtca cagcggcgca atctcttatt ttgtgcagca
      481 ggctgcccgc gccggattga ttggcatttc gctgtgccag tccgatccaa tggtggtgcc
      541 gtttggcggc gcggaaattt actacggcac taacccactg gcctttgccg cgccgggaga
      601 aggcgacgag atccttacct ttgatatggc gactaccgta caggcatggg gaaaagtact
      661 cgacgcccgc tcgcgcaata tgtctatccc ggatacctgg gcagtcgata aaaacggtgc
      721 accaacaacc gatccgttcg cggtacatgc tctgctcccc gccgctgggc caaaagggta
      781 tggcctgatg atgatgattg acgtcctctc aggcgtctta ctcggcttac cgttcgggcg
      841 acaggttagt tcgatgtatg acgatttaca cgccgggcgt aatttggggc aattacatat
      901 agttattaac ccgaactttt tctcctccag cgaattattc cgtcaacatc ttagccagac
      961 catgcgcgaa ttaaatacca ttacccccgc gcccggtttt aatcaggttt attatcccgg
     1021 acaggatcag gatattaaac aacgcaaagc cgccgtcgaa ggcatcgaaa ttgttgatga
     1081 tatttaccag tatttaattt ccgacgcgct ttataacacg tcatacgaaa cgaaaaatcc
     1141 ctttgcgcaa taattattga gacaggactt tcttatgatt acacatttcc gtcaagctat
     1201 agaagaaacg ctgccctggc tttcctcttt tggcgctgac ccagcgggtg ggatgacccg
     1261 tttactttat tcgccggaat ggctggaaac ccagcagcag tttaaaaaaa gaatggcagc
     1321 aagcgggctg gaaacacgtt tcgatgaagt ggggaattta tacggtcgcc tgaatggcac
     1381 cgaatatcca caggaagtgg ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg
     1441 taacctcgac gggcaattcg gtgcgctggc ggcgtggctg gcaattgact ggctgaaaac
     1501 gcaatacggc gcgccgttac gtacggtcga agtggtggcg atggcagaag aagaaggcag
     1561 ccgcttcccg tatgttttct ggggcagtaa aaatattttt gggctggcga atcctgacga
     1621 cgtgcggaat atctgtgatg ccaaaggaaa tagttttgtc gatgcgatga aggcttgcgg
     1681 atttactctg ccgaacgccc cactaactcc gcgtcaggat attaaagcct ttgtcgaact
     1741 gcatatcgaa cagggctgtg tgctggaaag taatgggcag tcaattggcg tggtgaatgc
     1801 aattgtcggg caacgtcgct atacggtgac gctgaacggt gaatcaaacc atgcaggcac
     1861 cacgccgatg ggttatcgtc gtgatacggt ttacgctttc agtcgcattt gccatcagtc
     1921 tgtcgaaaaa gcgaaaaaga tgggcgaccc gctggttctg acttttggca aagtagagcc
     1981 gcgcccgaat acggtgaatg tagtgccggg taaaaccacg ttcaccattg attgtcgtca
     2041 taccgatgct accgtgctgc gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat
     2101 ttgcgatgaa atggatattg gtattgatat cgatttatgg atggacgaag aacccgtgcc
     2161 gatgaataag gagctggtcg ccaccctgac agaattgtgt gaaagcgaaa aactgaatta
     2221 ccgggtgatg cacagtggtg ccgggcacga cgcgcaaatt ttcgcgcctc gcgtgccgac
     2281 ctgcatgatt ttcattccca gcatcaacgg gatcagccat aacccggcgg aacgcaccaa
     2341 tattaccgac cttgccgaag gggtcaaaac gttggcactc atgctttatc aacttgcctg
     2401 gcagaaataa ggagttataa atgggatatt taaataacgt caccggttac cgcgatgatt
     2461 tactggctaa ccgtgcgatt gttaaacacg gtaatttcgc actgttaaca ccagacggtc
     2521 tggtaaaaaa tattattccg ggctttgtaa attgtgacgc gacaatcctc tccacgccaa
     2581 agctgggtgc ctcttttgtt gattatctgg tcacactgca tcaaaacggc ggcaaccaac
     2641 agggcttcgg tggcgaaggc attgaaacgt tcctgtatgt gatctctgga aatatcactg
     2701 ccaaagccga agggaaaaca tttgccttaa gcgaaggtgg ctatctttat tgcccgccag
     2761 gctccttaat gacgtttgtt aacgcccagg ccgaagacag ccaaatcttt ttatataagc
     2821 gtcgctatat tccggtagaa ggtcatgcgc cgtggctggt ttatggcaat gccagcgaac
     2881 tggaacgcat tcattatgaa ggcatggacg atgttattct gctggatttt ctgcccaaag
     2941 agttaggttt tgatatgaac atgcatatcc tctcttttgc accaggtgcc agccacggtt
     3001 atatcgaaac ccacgttcag gaacacggtg cctatattct ttccggtcag ggggtttata
     3061 acctcgacaa taactggatc ccggtgaaaa aaggcgatta catctttatg ggcgcttatt
     3121 ctttacaggc tggttatggc gtagggcgtg gtgaagcgtt cagctatatt tactcgaaag
     3181 attgcaaccg cgacgtagag atttaagtca taccactcgc ctgattattt ttagctatgt
     3241 tggattttgc cacggtttat tccgtggctt tttttgtagt caattcaggt aaaaaaagat
     3301 gcgatataaa tcgcatccgc tttaaaggta atagtcagtt tttaattccc tgacctattt
     3361 taatggcgca ggcaatattt cgcgcactgt tgaagagatt ggtttcaccg ctggcgagca
     3421 cttcggctaa aggagccaga cgtggcaaaa tgctgaatac cgcatcaatg ccgtactggt
     3481 gcaccacttc cacgccatca cccaatacgc cagcaatccc aatcaccggg acattaaact
     3541 gcttcgccac cgatgccaca cccagcggcg ctttaccgcc tgccgtttgc gagtcgatgc
     3601 gcccttcccc ggtgatcacc agtgctgcac cctgcactgc ctgcgcaaga ttgaccgcat
     3661 tcaacacaat ctcaataccc ggtttaatat ccgcattgag gaataccgcc gccgcaatgc
     3721 ccataccgcc cgccgcgcca ccacccgcca tctggcagac gttgatttca gttagctgtt
     3781 gtaaaacacg ggcgtaattt tgcagcccct gttccagctc ttcgaccatc tccggcgttg
     3841 cccctttttg cgggccaaac accgccgccg cgccgcgtgc ccctaccagc gggttatcaa
     3901 cgtcacatgc tacttcaata tgacagttcg ccagacgcgg atcgcattca ttcatctcaa
     3961 tacttgccac gcgcgctaaa ttgccgccgt tagccgccag cggctgaccg tcggcatcaa
     4021 ggaaacgcac accgagcgcc tgcgccatgc ccataccgcc gtcgaccgtc gcactgccgc
     4081 caatgccgag aataatatgg cgaatgccat tatccagcgc atggagaatt aactcccccg
     4141 taccaaaact ggaggccagc aacggattac gcttttcagg cacgaccagc atcaggccac
     4201 ttgctgctgc catctcaatc accgccgttt tcccgtcgcc ggtaatgcca taaaaagcat
     4261 tcactttttc gcccatcggc ccgcagactt caagcttcac gatgttaccg tccgtcgcgg
     4321 cgaccatcgc ctccaccgtc ccttcgccgc catccgctat cggcaaacag atatagtgcg
     4381 catcgggaaa tatggtcgaa aacccggctt taattgcctg acaacatttt tctgcactta
     4441 agctctcttt aaaagagtct ggcgcaatga caatcttcat tatcaatcct ttttcaacaa
     4501 attattcgat gcgttcccac ggaataagat tatccataag aacagccagt aaaattccga
     4561 ccagtaagcc gttactgagc agaggacgaa gcgttaatgg aaggtcttgc agatacacag
     4621 gcggtaatgc cattaaaaat atgccgacaa ataacggcaa tgcgagtcga taaatattgc
     4681 gagcggtaaa cgttatttgc tggctaaaca ccagcgcgga aaagagtaaa ggcagataag
     4741 aaaccagcat gaccgcacta ctcactggta aagggatact gcaaaacagt cgcgtgagtg
     4801 ccggaaccag tgccaccagc aggcaaataa cgctgccata aataaacgaa cgccgcgagt
     4861 aatcgcctgt ctgggttaat aaaccaatgg atgaaacaaa cggtgaaaat ggaattactg
     4921 ccagcggcac ggttatcagt gtcataaatc cggtcgccac aaagctgcga cgataacgcg
     4981 tattccccgc gccctgctgc ggataaaaaa catccgtgcc ccgaatcgca ccgtaggtat
     5041 tgctgatatt taccagacct gtaatcaccg ccgtcagaat aatccccggc gacaaagcac
     5101 cgccactgcc gagcgggaac cactgccagt gcaactcacc ggagagcgaa tgcgaagaag
     5161 gaaagcaaaa gtaccacaac aaccagccag ttatcgtgcc gaccagcagg ccgtaacggg
     5221 caaaacgttg tggcaggaaa ataatcatcg ccagtaccag gcacatcacc gccaccgaga
     5281 gcgcgaacgg cggtaattga attttaaaat tcggggcggc tatgccaaac ggcagcccga
     5341 gcataccttt gaaaaagata gtggtcagtt gcgcacccag catcaacata aacaagacca
     5401 tcaccgacgg cgtaaataac cgtgccaggc gatggcctaa tccgctaaaa ccaatcaaca
     5461 tcgtcagcac gccggagagc gcaatcccca ccgccagact ggtagcgata tcgttgatcg
     5521 gtgtcccgcg cgatgcttcg ccaagggtga tagtcaggat ggttccccac cacaggccgc
     5581 ccggcccttc cataatagcg cgacgatgac cgcagaacgc ctgagcgaag caggccagtg
     5641 cggtagcaag aaaagcatat tgcgtgagcg taagcaggct actttgcggc aactgaaaag
     5701 cagaaagtag cgtaggagga accacaaccg tgttgcaaaa aataaagaaa aaccactgga
     5761 atcctgataa caggctttcg cggcccactg caaaattgaa cataaacaca ccttaaccgg
     5821 agaatgcccg ccacaaggac gggcattgca gggccagatt actgctgatg tttaaggata
     5881 aattgacctt tcggcgcaac agggaagccc tgttcgatgt cgtaaatcac atcaccacgt
     5941 aagatggttt tcgtgatacg cgcgccaata gtacggccaa catacgggct gactttgtgg
     6001 cgatattcca gatcgtcatt ggtaagaaca tagctgctat tcggctgaat gaagacgaag
     6061 tcggcatctt ttcctggggc gatacggcct ttttgctgca gaccgaaaat atctgctgcg
     6121 ttagtcgcca ttaatttgcc gaacattggc agagacattc cgcgtttctg taccgcttca
     6181 tcgaacatca cgtccatgca gctttgcaga ccagcgatac cgccccacgc tttcatgatg
     6241 ttaccggctt tcatttccgg cgggcatgga gagtggtcgg aaaccaggca gtctatttca
     6301 ccgttaaaca gtttttccca catgcctttc tggttttcca gatcgcggat cggcggtgaa
     6361 cacttcgcca gagtaccgat ttcttcgaac tgatcggtat ccagcacaaa gtaatgcggg
     6421 caggattcac aagtaacatc ctgaccttcc tgacgtgcac gagtcacttc ctcaacacct
     6481 tccgggctgc tgacgtggca aacgtgcaga cggcaaccag cgactttcgc caggtacagt
     6541 acgcggcgaa tcgcttccac ttcggtaaat accggacgcg aagccacata gtcatgggca
     6601 gttacgcgac cttcacgctt cgcttcttcg cccagtgcgt cacagatcag cgcgttttcg
     6661 cagtgcacca gcactggctg gcccagttcg cccagcttct gcgcgccttt gaagaattgc
     6721 cagtcattga cgtcacggaa gtcgttgtcg ataccgcgat cgccacaggt cgcaacgaag
     6781 catttgaaac cgacaacacc cacttcatcc aactcatgca gacggtcaat gttgtaagac
     6841 accaggccac cgagttgtgc cgcatcgata gtcagctttc ctttagcggc atcgaacttc
     6901 agttcaattg aggcgcggtc aaccgttgca ggcagctggt tgagcggcat ttcgatcatg
     6961 gtggtgatac caccttttgc tgctgcgcga gtaccggttt cataaccttc ccagtggctg
     7021 cgacccggtt cagaaatatg ggtgtgcgca tcaaccatgc ccggcgaaac caccagacca
     7081 gacgcatcca taacgtcttt tgcatcgccc agatcctggc cgatagcagc aatttttccg
     7141 tctttaacgg cgatatctac aacgcgagct tcgttttcta aaataacggt gccgttttta
     7201 atgattaaat caaaagacat aacaaactcc ttgtaaaata cagattggtt tttaactcaa
     7261 tgttcaatat cgtgattaat taacctatgg ttttttgttc tcctgttttt tctgctgttg
     7321 tacgtttctt taataaggcg taggccgcaa aggcgacgat gacgccgaca aaccatgaaa
     7381 cacgtgataa cggttccata aagggaataa acttaccgcc aagagataaa ataacggcca
     7441 ccagagttac tgaaaacgcg gtgaggttaa aaccgttatc gtaatattta taatcgccag
     7501 gtgcggtgta cagttcatca agattaattt gcccacgcat caccacaaaa taatgcgcca
     7561 tcatcacacc aattaccgga ccaagcattc cgccgataat atcgaggaac agataaatac
     7621 tgtcctgatt ttccattaat ttccacggac agatcagcag gctgataata ctggcaatca
     7681 gtacgccgtt tttataggtc agtttcgtcg gtgcaatggc ggcaatctga taaccagccg
     7741 gaataatatt accggtggcg ttagtagaga tagtcgtcat cagaataacc agtaccgcaa
     7801 agaacgaggc gaacaggctg tcccaacgct gaacaatatc cagcacgttc caggtgtcag
     7861 cgccgtaatg aatactggct ccggcaataa tacatacccc ggcgaccgca aacagaatat
     7921 aggccacaac taaacccagc gtttgcccca gcgcctgctc acgaaacgag tgggcgtttt
     7981 gcgtaaagtc ggatgcgctc accgccggtg ccgcccagac cgcaaccacc gcgtttatca
     8041 ccaccagaaa caggaagcca ctgttttctg ctttctgaat accgcccgga atgtagtcaa
     8101 agattggacc gatcccgacc agcgaaatcg cccaaatcgc cataccgccg aaaacgatat
     8161 agatgcacgg gttaagaatg gcagtgaatt tatttaaaac ttttccgccg ccaaaaccaa
     8221 taccaacgtt aaccagccag aagagtaaga aagtaattaa tcccggtaga gaaaggccta
     8281 acagcgtgaa atcgccaccg agagtcaaaa atcccggcca gattttgcca atcagaatca
     8341 agcaggccag tgaccccgcg taacattgca ggccaaacca cataatggca gcaataccgc
     8401 ctcttaataa tccgggaaac agtgcaccac gtacgccgta agaagcacgc aggatcatgg
     8461 caaacggtac gccgtattta ctgcccgcag caccgtttaa taccattacc gcggcaatga
     8521 aaaaggcact gaggataatt gccagcataa tactaaaggt agacaagccg agaataaaaa
     8581 agccgccaac catcacataa ttgggaacgt tatgaaccga acccatccat aaggtaaaat
     8641 agttaaatgt tttccaggtc cgctgacttt gcgttttcgg cagtagatct tcgctatagc
     8701 cgcgttgctg gaatagtttt ctctgatgtt ccataaatcc ctcttaattt cacgtatcga
     8761 a
//
LOCUS       DACADF010000175         8729 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.177, whole genome shotgun sequence.
ACCESSION   DACADF010000175 DACADF010000000
VERSION     DACADF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.177"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..166
                     /locus_tag="ISB30_004153"
     CDS             <1..166
                     /locus_tag="ISB30_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP1991722.1"
                     /translation="YTRQGYAIEAITATIDWIKENGFSLIKAGVNPENTLSKKLLIRI
                     GFNFSSIEDG"
     gene            543..800
                     /locus_tag="ISB30_004154"
     CDS             543..800
                     /locus_tag="ISB30_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991723.1"
                     /translation="MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLT
                     DCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRAQLDNR"
     gene            812..1363
                     /locus_tag="ISB30_004155"
     CDS             812..1363
                     /locus_tag="ISB30_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1991724.1"
                     /translation="MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDES
                     ARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMHILAPLAVIPEYQGMGVGGRLIR
                     TGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
                     QPMNVTGHIQCAQALMKPEHWRE"
     gene            1415..2161
                     /locus_tag="ISB30_004156"
     CDS             1415..2161
                     /locus_tag="ISB30_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP1991725.1"
                     /translation="MDIPRIFTISESEHRIHNPFTEEKYATLGRVLRMKPETRILDLG
                     SGSGEMLCTWARDHGITGTGIDMSSLFTAQARRRAEELGVSERVHFIHNDAAGYVANE
                     KCDVAACVGATWIAGGFAGTVELLAQSLKPGGIMLIGEPYWRQLPATDEIAQACGVSS
                     TTDFLTLPGLVSAFDDLGYDVVEMVLADQEGWDRYEAAKWLTMRRWLEANPDDDFAAE
                     VRAELNISPKRHVTYAREYFGWGVFALIAR"
     gene            2289..2549
                     /locus_tag="ISB30_004157"
     CDS             2289..2549
                     /locus_tag="ISB30_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991726.1"
                     /translation="MSQHEQFCQACGMPMSAPDAHSASDQYCAYCSDSNGNLKPWEEA
                     VSGLASFLDSWQKVGPEEAHKRAKRYLTAMPAWAHKAESDLS"
     gene            2948..4069
                     /locus_tag="ISB30_004158"
     CDS             2948..4069
                     /locus_tag="ISB30_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP1991727.1"
                     /translation="MSFSVQETLFSLLQHNAISGHENAVADVMLCEFRRQAKEVWRDR
                     LGNVVARYGSDKPDALRLMIFAHMDEVGFMVRKIEPSGFLRFERVGGPAQVTMAGSIV
                     TLTGDKGPVMGCIGIKSYHFAKGDERTQSPSVDKLWIDIGAKDKDDAIRMGIQVGTPV
                     TLYNPPQLLANDLVCSKALDDRLGCTALLGVADAISTTELDIAVYLVASVQEEFNIRG
                     IVPVLRRVKPDLAIGIDITPSCDTPDLHDYSEVRINQGVGITCLNYHGRGTLAGLITP
                     PRLIRMLEQTALEHNIPVQREVAPGVITETGYIQVEQDGIPCASLSIPCRYTHSPAEV
                     ASLRDLTDCIRLLTALAGMSAAHFPVEPDSGTTQEAHPL"
     gene            4066..4344
                     /locus_tag="ISB30_004159"
     CDS             4066..4344
                     /locus_tag="ISB30_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP1991728.1"
                     /translation="MKKILVACGTGMSTSTMIAHKLQEFLTEQGISATTAQCCLNEIP
                     LNCNGMDLIVTSMRTNSDYGIPTLNGAALLTGINDDALKQQIKALLTQ"
     gene            4356..5669
                     /locus_tag="ISB30_004160"
     CDS             4356..5669
                     /locus_tag="ISB30_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP1991729.1"
                     /translation="MFDYILSLGGTVFVPIIMIVIGLIFRIPWLQAIKAGVTVGIGFV
                     GMGLVIVMAIDSLSPPIKVMIERFGLALHVFDVGAGPASGVGYATAIGAMIIPVIFLL
                     NVAMLVTRLTKTMNVDIYNYWHYAITGTVVQLMTGSLIYGVLGAICHAALSLKMADWT
                     AKRVQNIVGLEGISIPQGYGSSSVPLFVLLDAIYEKIPFMKGRNIDAQEIQKRYGMVG
                     DPVIIGVVLGLIFGLAAGEGFKGCASLMITVAAIMVLFPRMIRLIVEGLMPISDGARK
                     FFQKYFKGREVYIGLDTAVTLGHPTTIAVGLLLIPIMLILASILPGNKVLPLADLPVA
                     PFFICMATVIHRGDLVRTLISGVIVMITVLLIATQFAPYFTEMALKGGFSFAGESAQI
                     SALSVGNMFGWSISELMSLGIIGVVVAVGIVASVVLFLRKRELSE"
     gene            5682..6488
                     /gene="sgcQ"
                     /locus_tag="ISB30_004161"
     CDS             5682..6488
                     /gene="sgcQ"
                     /locus_tag="ISB30_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein SgcQ"
                     /protein_id="HAP1991730.1"
                     /translation="MSWLKEVIGTEKAVIAMCHLRALPGDPSFDAQLGMNWVIDKAWD
                     DLMALQNGGVDAVMFSNEFSLPYLTKVRPETTAAMARIIGQLMSDIRIPFGVNVLWDP
                     VASFDLAMATGAKFIREIFTGAYASDFGVWDTNVGETIRHQHRIGAGEVKTLFNIVPE
                     AAVYLGNRDICSIAKSTVFNNHPDALCVSGLTAGTRTDSALLKRVKETVPDTVVLANT
                     GVCLENVEEQLSIADGCVTATTFKKDGVFANFVDQARVSQFMEKVHHIRR"
     gene            6619..7050
                     /locus_tag="ISB30_004162"
     CDS             6619..7050
                     /locus_tag="ISB30_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP1991731.1"
                     /translation="MINDIKWVQAQREATDWRQAVEIATRPLVAYGAAQPCYVNGIIE
                     NTLNWGPYYLIAPGIALPHARPEQGANYNQVSITTLRTPVAFGNEECDPVWLLLCVSA
                     TDANAHILTIQRISQFIDSPQRLAAVGNASTDDALFALVSG"
     gene            7062..7694
                     /locus_tag="ISB30_004163"
     CDS             7062..7694
                     /locus_tag="ISB30_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3 epimerase family protein"
                     /protein_id="HAP1991732.1"
                     /translation="MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNIT
                     FGMKTVQAVARQTPHPLSFHFMVARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEI
                     RHTGARAGLVFNPATPIDAWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTA
                     FPQTECWADGGITLAAAQQLAAAGAQHMVIGRALFSSSDYRATLAQFATL"
     gene            7711..8493
                     /locus_tag="ISB30_004164"
     CDS             7711..8493
                     /locus_tag="ISB30_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1991733.1"
                     /translation="MSQQRPDRIKQMLHYLWQHRHLSTQQAMELFGYAEATVRRDFQY
                     IANQYPGMIRGHGCLDFDDSTDDKEYVFDVKRTLQSVAKREIAALARTMIKDGDCFFL
                     DSGSTCLELAKCLADARVKVICNDIKIANELGCFPHVESYIIGGLIRPGYFSVGESLA
                     LEMINAFSVERAFISCDALSLETGITNATMFEVGVKTRIIQRSREVILMADHSKFDAV
                     EPHAVATLSCIKTIISDSGLPETIAQRYQRAGCQLFLPHSIK"
BASE COUNT         2091 a         2242 c         2251 g         2145 t
ORIGIN      
        1 ttatactcgc cagggatatg caattgaagc aattaccgcc acgattgact ggataaaaga
       61 gaacggtttt tcactcatca aagccggtgt gaatccggaa aatacccttt caaaaaaatt
      121 acttatcagg attggcttca atttttcgag catcgaagat gggtgagcat atttatgttc
      181 tggattaaac aaaaggagaa taatctcccg aagggaaaag ccgatgaggc catttcaggc
      241 gtacttcaac atgcattcgt cagtgtcatc tgccttttcc agtaaatggg ttggcattca
      301 atttccaaag tacatcacac tttttctact gaaacatatc cctcctataa ctacgtgtta
      361 atattcgctc cgtaaatacc tttcaactca acccgaggct ttgatgctgg agaatgtaat
      421 catccgataa tcagttcccc gaccttttca ggccggactg attatcaatg cgccgaaatc
      481 gaatgcggac accgcggtgt gtttgcacgt tttgcacatg atgattaaac aggttcttca
      541 gtatgaattt atcccgtcag gaacaacgta cgttacacgt tctcgctaaa ggtggacgta
      601 ttgcgcacgt ccgcgattct tcaggccgcg tcacttccgt tgaatgctac agccgcgaag
      661 ggctgttgct gaccgactgc acgctcgccg tcttcaaaaa actcaaaacc aaaaaactta
      721 tcaagtccgt caatggtcag ccctatcgaa tcaacactac cgggctgaat aacgttcgcg
      781 ctcagctcga taatcgctaa ggagagtttc gatgacagca caccacttca cttttcaaat
      841 cacagatgaa agtgatgcca gtgatatccg ggaagtcgaa acccgcgcat ttggcttcag
      901 caaagaagcc gatttaacgg cctctttact tgaggatgaa agcgcgcgcc ctgcgttatc
      961 gctgctggcc cgacatgaag gcaaagccgt cgggcatatt ttatttaccc gggcaacctt
     1021 caaaggggaa atggattcac cgctgatgca tatcctcgct cccctggcag tcataccgga
     1081 ataccagggg atgggcgtgg gaggacggtt gatacgcact ggcatcgagc acctgaggtt
     1141 gatgggctgc cagacggttt ttgttctcgg ccacgccacc tattaccccc gacatgggtt
     1201 cgaaccctgt gccggagaca aaggctatcc ggcaccttat cccatcccgg aggagcacaa
     1261 agcctgctgg atgatgcaag cactcaccgc tcagcccatg aacgtgaccg gccacatcca
     1321 gtgcgcgcag gccctgatga aaccggagca ctggcgtgaa taacaatctt gttgtgccct
     1381 ttttaatgct taacccaata acaggaatta actaatggat attccacgta tttttaccat
     1441 cagtgaaagc gaacaccgca tccacaaccc gttcaccgaa gagaagtacg ccacgctggg
     1501 tcgcgtgctg cgcatgaaac cagagacccg aattctggac ctcggcagcg gctcgggcga
     1561 gatgctctgc acctgggccc gggatcatgg cattacgggg actggaatcg acatgagttc
     1621 gctcttcacc gcgcaggcca gacgccgcgc ggaagaactc ggcgtcagcg aacgcgtaca
     1681 ttttattcat aatgatgcgg cgggttacgt cgccaatgaa aaatgtgacg tagcagcctg
     1741 cgttggcgcg acctggattg ccggaggctt tgccgggaca gtcgaactgc tggcgcagag
     1801 ccttaaaccg ggcgggatca tgctcatcgg tgaaccttac tggcgtcagc ttccggcaac
     1861 ggacgagata gcccaggcct gtggcgtcag ctcgacgacc gattttctga cgctaccagg
     1921 gcttgtcagc gcgtttgacg acctcggcta cgacgtggtg gaaatggtac tggcggacca
     1981 ggaaggctgg gaccgatatg aggccgcgaa atggctgacc atgcgccgct ggctggaggc
     2041 caatcctgac gatgacttcg ccgccgaagt cagggccgag ctaaacatct cgccgaaacg
     2101 ccacgtaacg tacgcgcggg aatactttgg ctggggcgtg ttcgcactca tcgctaggta
     2161 acaacaggtt cccggtattt ctgactggag ataccggggg cttagccttt gctttaatct
     2221 gatgcatcac ctgcacaata catattcagt atgtttatta atcaagatgt caccataagg
     2281 agcatcatat gtcacaacat gaacagttct gtcaggcttg cggaatgccg atgtcggcac
     2341 cagatgcaca cagtgccagt gatcaatatt gtgcgtactg cagcgacagc aacggcaatc
     2401 tcaaaccctg ggaagaagcg gtatcaggtt tagccagctt ccttgattcc tggcaaaaag
     2461 tcggcccgga ggaagcacat aagcgtgcaa aacgttatct gacggcaatg ccagcctggg
     2521 ctcataaagc ggagagtgat ttgagctgat tatctatact ccctctgaat ctattcagga
     2581 gatccgatgt tttcttacat tatgctggga accaacgacc tgccacgcgc cattacattc
     2641 taagatccgc tgatggagat gctggggtat ccacgtgcag gacgtaatga agagggagcg
     2701 tcctgcaaca aagtggtgcg ataacagtta ccgcgtttta ttcattctca ttatttcaac
     2761 cataatcact ggtctctcta gcccgtaact ttcacaaacg ttgagcgctt ttattccact
     2821 cggctcacat aattttttat ttgttcagct ttgcgcgttt tcacgtgaac gttctcacaa
     2881 ttattcgacc agtctgcgcc tataaaacgc tcaacgagtg attgttttac ggtctacgga
     2941 gatagctatg tcattttctg tgcaggaaac cctgttctcg cttttgcagc acaatgcgat
     3001 ttcaggacac gaaaacgctg tcgctgacgt catgctgtgc gaattcaggc gtcaggcaaa
     3061 agaggtctgg cgagacaggc ttgggaatgt cgtcgcgcgc tacggtagtg ataaacccga
     3121 tgcgctgcga ctgatgattt ttgcgcatat ggatgaagtc ggttttatgg tgcgcaaaat
     3181 agagccttct ggatttttac gctttgaacg cgtaggcggt cctgcgcagg tcactatggc
     3241 tggatccatc gtcaccctca ccggggacaa agggccagtc atggggtgta tcggcattaa
     3301 gtcctaccac tttgccaaag gcgacgagcg cacgcagtca ccttctgtcg acaaactgtg
     3361 gattgatatt ggtgccaaag acaaagacga cgctatacga atgggcattc aggtcggtac
     3421 gcctgtcact ctgtataacc cgccgcaact cctggcaaac gatctggtgt gcagtaaagc
     3481 actagacgat cgtctgggct gtactgccct gctcggtgta gcggatgcta tcagtactac
     3541 ggagcttgat atcgccgttt atctggtggc ttcggtacag gaagaattta atatccgcgg
     3601 cattgttccc gtattacgcc gtgtaaaacc tgacctggcg attggtattg atatcactcc
     3661 atcgtgtgac acccctgatt tacacgatta ttccgaggtc aggattaatc agggcgttgg
     3721 gatcacctgc ctgaactacc atggtcgggg aacgctggcc ggattaatca cgcctcctcg
     3781 cctgatacgg atgttggaac agacggctct tgaacacaac attccggtgc agcgagaagt
     3841 ggctcccggc gtgataacag aaaccggtta tatccaggtt gagcaggatg gtattccctg
     3901 cgccagtctc tctattcctt gtcgctatac ccattctccg gcggaggttg ccagcctgcg
     3961 tgatttgact gattgcatcc gcctattgac cgctctggca ggtatgtcag cagcacattt
     4021 ccccgttgag cctgattcag gcactacaca agaggcacat ccattatgaa aaagatcctt
     4081 gtggcatgcg gtaccggcat gtcgacttca accatgattg cacacaagct gcaagagttc
     4141 ctgactgaac agggcatttc agccactacc gcccaatgct gtctgaatga gatccctctt
     4201 aactgtaacg gtatggacct tattgtaacc tccatgcgaa ccaacagtga ttacggcatc
     4261 cctacgctta atggcgccgc cctgctcaca gggatcaacg acgacgcgtt aaaacaacaa
     4321 atcaaggctc tgttaactca ataaaaggag gcattatgtt tgattacatc ctgtctctcg
     4381 gcggtaccgt tttcgtccct atcatcatga tcgtgattgg ccttatcttc cgcatcccct
     4441 ggctgcaggc gattaaggca ggggtaacgg ttgggattgg ttttgtcggt atgggactgg
     4501 tgatcgtcat ggcgatcgac agcctcagtc cccctatcaa agtgatgatt gagcgtttcg
     4561 gcctggccct gcatgtcttt gacgttggcg caggccctgc ctccggcgtt ggttatgcga
     4621 cagccattgg cgccatgatt atcccagtta tttttcttct caatgtcgcc atgctggtca
     4681 cccgtctgac caaaacgatg aacgtcgata tttacaatta ctggcactac gccattacgg
     4741 gtacggtggt ccagcttatg accggtagcc tgatttatgg tgtgctgggg gcaatctgcc
     4801 atgcggcgct atcgttaaaa atggccgact ggacggcaaa acgggtacag aacatcgtcg
     4861 ggctggaagg tatttcgata ccgcaaggct acggttccag ttcagtaccg cttttcgttc
     4921 tacttgatgc tatctatgaa aaaatccctt tcatgaaggg acgcaatatc gatgctcagg
     4981 agatccagaa acgctacggc atggtcggcg atccggtgat tatcggcgta gtgctcggtc
     5041 tgatttttgg cctggcggct ggtgaaggct ttaaaggttg cgccagtctg atgattaccg
     5101 ttgcggcaat catggtgctg ttcccgcgca tgatccgcct gattgttgag ggcctgatgc
     5161 ccatctccga cggtgcaaga aagttcttcc agaaatattt caaaggacgt gaagtgtata
     5221 tcggtctgga tactgctgtg acgctggggc atccaaccac cattgctgtc ggtctgctac
     5281 tgatacccat tatgttaatt ctggccagca ttttaccggg caacaaagtg ttacccctcg
     5341 cagacctgcc ggtagcaccg ttctttatct gtatggctac ggttatccac cgcggagacc
     5401 tggtcaggac gttgataagc ggggtcatcg tgatgattac cgtcctgctg atcgccaccc
     5461 agttcgcgcc ttacttcact gaaatggcgc tcaaaggcgg tttcagtttt gccggtgaaa
     5521 gtgcgcaaat ctcagcgttg tccgtgggca atatgttcgg ttggtcgatt tctgaattga
     5581 tgtcgcttgg gattattggc gttgtcgtgg cggtcggtat tgtcgccagc gtcgtgctgt
     5641 tcttacgtaa acgtgaatta tcggaataag gagtactttc aatgagttgg ctgaaagagg
     5701 ttattgggac ggaaaaagcg gtcatcgcca tgtgccatct acgtgcattg cccggcgacc
     5761 cgagttttga tgcgcagttg gggatgaact gggttatcga caaagcctgg gacgatctga
     5821 tggcgctcca aaatggtggg gtggatgcag tcatgttctc caacgagttt agtcttccct
     5881 atctcacgaa ggtgcgtccg gaaaccactg cggcaatggc acgaatcatc gggcaattaa
     5941 tgagcgatat ccgcatcccg tttggcgtga atgttctgtg ggatccggtc gcctcattcg
     6001 acctggcgat ggctaccggc gctaagttta tccgcgagat atttaccggt gcctatgcca
     6061 gcgactttgg cgtctgggac actaacgtcg gtgagaccat ccgtcatcaa caccggattg
     6121 gtgcaggcga agtgaaaacc ttgttcaata tcgtcccaga ggccgccgtg tatctgggca
     6181 atcgcgatat ctgttctatt gctaaatcga cagtatttaa caaccatcct gatgcgctct
     6241 gtgtttctgg cctgaccgcc gggacgcgta cagacagcgc gctgttaaaa cgggtcaaag
     6301 aaacggtgcc tgacaccgtc gtgttggcca acacgggcgt ctgcctggaa aacgtggagg
     6361 aacaactcag tatcgccgat ggctgtgtga ccgcaaccac ctttaaaaag gacggcgttt
     6421 ttgccaattt cgttgaccag gcgcgagtca gtcagtttat ggagaaagtg catcatatac
     6481 gccgataaga tagggataag cagagcgctt atccccggca aagagcaata acccttctgc
     6541 gatccctgcc gccttcgtca aaagtatcga cggggatcgc taccagatta acccgaatcg
     6601 gcatggagtc aacgacggat gattaacgat attaaatggg tacaggcaca acgtgaagcc
     6661 accgactggc gtcaggccgt ggaaatcgcc acccgcccgc tggtagccta tggtgccgcg
     6721 cagccatgct acgttaatgg gatcatcgaa aataccctta actgggggcc ttattatctg
     6781 atagccccag gaatcgcctt gcctcacgcc cgaccagagc aaggcgcaaa ctataatcag
     6841 gtcagtatca ccacgctacg caccccggtc gcctttggta acgaggagtg cgaccccgtc
     6901 tggctgcttc tctgcgtcag cgccacagat gccaacgcac atattttgac cattcagcgc
     6961 atcagccagt ttattgattc gccacaacgc ttagcggcag tgggaaatgc cagcacagac
     7021 gatgcactgt ttgcgctggt atcaggttaa cggaggcgct tatgatcctg cacccttctc
     7081 tcgccagcgc aaatcctctg cactatggtc gtgagctgac ggcattggac aatctggatt
     7141 ttgggagcct gcatctggat attgaggaca gcagcttcat taacaacatt acgtttggga
     7201 tgaaaacggt gcaggccgtc gccagacaga cgcctcatcc cttgtccttt catttcatgg
     7261 ttgcccggcc acagcgctgg tttaacgcgc tggctgaaat cagacccgcg tggatcttcg
     7321 ttcatgcaga aacgctggac tatccttcgg aaacgttaac tgagatccgc cataccggcg
     7381 cacgagccgg actggtattt aatccggcaa ccccgatcga cgcctggcga tatctggcgt
     7441 ctgagctgga cggagtgatg gtgatgacca gtgagcccga tgggcaggga cagcggttta
     7501 ttccctccat gtgcgagaaa atccaaaaag tacgcacggc ttttcctcag accgagtgct
     7561 gggccgacgg tggaattacg ctggcagctg cacagcaact ggcagcagcc ggggcgcaac
     7621 atatggtgat tgggcgcgcc ctgttctctt catctgacta tcgcgcgacc ttagctcaat
     7681 tcgccaccct gtaagaggag attgtttctg atgagccaac agagacctga ccgtatcaaa
     7741 cagatgctgc actacctgtg gcaacatcgt cacctctcca cacagcaggc catggagctt
     7801 tttggctatg cggaagcgac tgtccgtcgt gatttccagt atattgccaa ccagtatcca
     7861 ggcatgatcc gcggtcacgg ttgcctggac ttcgacgaca gtacagacga caaagagtac
     7921 gtcttcgacg tgaaacgtac attgcaatct gtggcgaaaa gagaaatagc tgcccttgcc
     7981 cgaacaatga tcaaagatgg cgactgcttt tttctcgact ccggctcaac ctgcctcgaa
     8041 ctggcgaaat gcctggccga tgcgcgggta aaggtcatct gcaacgacat taaaatcgcc
     8101 aatgaactgg gctgttttcc ccatgtggaa agttacatta tcggaggact gattcgccca
     8161 ggttattttt cggtaggaga gagtctggcg ctggagatga tcaatgcctt ttccgtagaa
     8221 cgtgcgttta tctcctgcga tgcgctgtcg ctggagacgg ggatcaccaa tgccaccatg
     8281 tttgaggtcg gggtgaaaac acggattatt caacgctcgc gtgaagttat tctcatggcc
     8341 gatcactcaa aatttgatgc cgttgagccg catgctgtag caacactatc atgtattaaa
     8401 acgatcatca gtgacagcgg gttacctgag actattgcac agcgttatca gcgagccgga
     8461 tgtcagttgt ttttacctca ttcaataaag tgataagtaa gttcgttcga gagggatttc
     8521 aagcaaaaat aatcaatggc acccaataga aaatattggc gatgcgctcg aacgaataaa
     8581 gaagccctat gcgcaatcca cacgctacga tgttgcaaca acacgccatc tactttttat
     8641 tctcattcac taaatgtggt tgttctggat tcattattca aagtgtgtac aagatcacat
     8701 ttaatcactt cattacagtt cagcatgct
//
LOCUS       DACADF010000176         8560 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.178, whole genome shotgun sequence.
ACCESSION   DACADF010000176 DACADF010000000
VERSION     DACADF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.178"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            157..1278
                     /gene="ompC"
                     /locus_tag="ISB30_004165"
     CDS             157..1278
                     /gene="ompC"
                     /locus_tag="ISB30_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAP1991734.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVDGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNGYGERYLGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWA
                     NKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNRFTRDAGINTDDIVALGLVYQF"
     gene            1390..2445
                     /gene="apbE"
                     /locus_tag="ISB30_004166"
     CDS             1390..2445
                     /gene="apbE"
                     /locus_tag="ISB30_004166"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAP1991735.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITIGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KAWMSPQFKSFLISEKN"
     gene            2519..3583
                     /gene="ada"
                     /locus_tag="ISB30_004167"
     CDS             2519..3583
                     /gene="ada"
                     /locus_tag="ISB30_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAP1991736.1"
                     /translation="MKYAIRLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGSLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3583..4233
                     /gene="alkB"
                     /locus_tag="ISB30_004168"
     CDS             3583..4233
                     /gene="alkB"
                     /locus_tag="ISB30_004168"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAP1991737.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKVGGHPLTTDCRYNLTFRQAGKKE"
     gene            4309..5952
                     /gene="yojI"
                     /locus_tag="ISB30_004169"
     CDS             4309..5952
                     /gene="yojI"
                     /locus_tag="ISB30_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAP1991738.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFILVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6170..7816
                     /gene="mqo"
                     /locus_tag="ISB30_004170"
     CDS             6170..7816
                     /gene="mqo"
                     /locus_tag="ISB30_004170"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HAP1991739.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEVLKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(7966..8454)
                     /gene="eco"
                     /locus_tag="ISB30_004171"
     CDS             complement(7966..8454)
                     /gene="eco"
                     /locus_tag="ISB30_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAP1991740.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         2064 a         2127 c         2303 g         2066 t
ORIGIN      
        1 cgataaatga aacatcttaa aagttttagt atcatattcg tgttggatta ttctgcattt
       61 ttggggagaa tggacttgcc gactgattaa tgagggttaa tcagtatgca gtggcataaa
      121 aaagcaaata aaggcatata acagagggtt aataacatga aagttaaagt actgtccctc
      181 ctggtcccag ctctgctggt agcaggcgca gcaaacgctg ctgaagttta caacaaagac
      241 ggcaacaaat tagatctgta cggtaaagta gacggcctgc actatttctc tgacaacaag
      301 tcagaagacg gcgaccagac ctacatgcgt cttggcttca aaggtgaaac tcaggttact
      361 gaccagctga ccggttacgg ccagtgggaa tatcagatcc agggcaacag cgctgaaaac
      421 gaaaacaact cctggacccg tgtggcattc gcaggtctga aattccagga tgtgggttct
      481 ttcgactacg gtcgtaacta cggcgttgtt tatgacgtaa cttcctggac cgacgtactg
      541 ccagaattcg gtggcgacac ttacggttct gacaacttca tgcagcagcg tggtaacggc
      601 ttcgcgacct atcgtaacac cgacttcttc ggtctggttg acggcctgaa ctttgctgtt
      661 cagtaccagg gtaaaaacgg tagcgtagat ggcgaaggca tgaccaacaa cggtcgtggt
      721 gccctgcgtc agaacggcga cggcgttggc ggatctatca cttatgatta cgaaggcttc
      781 ggtatcggtg ctgctgtttc tagctccaaa cgtactgatg atcagaactt cggcctgaac
      841 ggttatggtg agcgttattt gggtaacggc gaccgtgctg aaacctacac tggtggtctg
      901 aaatacgacg ctaacaacat ctacctggct gctcagtata cccagaccta caacgcaact
      961 cgcgtaggtt ccttgggttg ggcaaacaaa gcacagaact tcgaagctgt tgctcagtac
     1021 cagttcgact tcggtctgcg tccgtctgta gcctacctgc agtctaaagg taaaaacctg
     1081 ggtgtcatca atggtcgtaa ctacgacgac gaagatatcc tgaaatatgt tgatgttggt
     1141 gcgacctact acttcaacaa aaacatgtcc acctacgttg actacaaaat caacctgctg
     1201 gacgacaacc gcttcacccg tgacgctggc atcaacactg atgacatcgt agctctgggt
     1261 ctggtttacc agttctaatc tcgattgata tcgaacaaag ggcctgcggg cccttttttc
     1321 attgttttca gcgtacaaac acagtttttt ggtgtactct tgcgaccgtt cgcatgagga
     1381 taatcacgta tggaaataag ctttacccgc gtggcactgc tggctgccgc gctcttcttt
     1441 gttggttgcg atcaaaaacc acaacccgcc aaaacccacg ctactgaagt taccgttctt
     1501 gaaggcaaaa ccatgggtac cttctggcgt gccagcatcc cgggcattga cgccaaacgc
     1561 agtgccgaac ttaaagaaaa gattcagacc cagctggacg ctgacgatca gctgctttcg
     1621 acctataaaa aagattccgc gctgatgcgc tttaacgact cgcaaagttt gtcgccgtgg
     1681 ccggtaagtg aagcgatggc cgatatcgtc accacctcgc tgcgcattgg cgcaaggacc
     1741 gatggtgcga tggatataac catcgggccg ctggtgaatc tgtggggctt tggcccggaa
     1801 caacaaccgg ttcaaattcc gagccaggaa cagatcgatg cgatgaaagc caaaaccggc
     1861 ttacagcacc tgacggtcat taatcagtcg catcagcaat atctgcaaaa agacctgccg
     1921 gatttatatg tcgatctctc caccgtcggc gaaggttatg cggcggatca cctggcacgc
     1981 ttgatggagc aggaagggat ttcccgctat ctggtgtcgg tgggcggcgc gctgaacagc
     2041 cgtggtatga acggtgcagg ccaaccgtgg cgggtagcga ttcaaaaacc aaccgataaa
     2101 gaaaacgcgg ttcaggccgt ggtggatatc aacggtcatg gtattagcac ctctggcagc
     2161 taccgtaact attacgaact ggacggcaaa cgtctttctc atgttatcga tccgcaaacc
     2221 gggcgtccta tcgaacacaa tctggtatcc gtgacggtga ttgccccgac ggcgctggag
     2281 gccgatgcct gggatactgg cttgatggta ctcggaccgg agaaagccaa agaggttgtt
     2341 cgccgggaag gactggcggt ctacatgatc accaaagagg gcgataattt taaagcctgg
     2401 atgtcgccgc agtttaaaag cttccttatc agcgaaaaaa attaaagcgc aagattgttg
     2461 gtttttgtgc gatggtgacc gggcagacta aaggctatcc ttaaccagag agctgattat
     2521 gaaatacgcc atacgcttaa ctgacgatca acgctggcaa tctgtcttag cccgcgactc
     2581 gaatgccgac ggcgaattcg ttttcgccgt gcgcaccacg ggtatctttt gccgtccgtc
     2641 ttgccgagcc agacatgcct tgcgggaaaa tgtctccttc tacgcaaatg ccagcgaggc
     2701 gcttgccgct ggctttcgtc cctgcaaacg ttgtcagcca gacaaagcca atcccctgca
     2761 acatcgcctt gataaaatta cccacgcctg ccgacttctg gaacaggaaa cgcctgtaac
     2821 gctggaagcc ttagccgacc aggtggcgat gagtccattt catttgcatc ggttgtttaa
     2881 agcgactacc ggaatgacgc ctaaagcctg gcaacaggcc tggcgcgctc gccgtttgcg
     2941 cgaattgctg gcgaaagggg agagcgtgac tacatctatt cttaacgccg gattccccga
     3001 cagcagcagt tactaccgca aagccgacga aacgctgggc atgacggcta aacaattccg
     3061 tcacggtggc gaaaatctgg cggtgcgtta cgcgctggct gattgtgagc tgggccgttg
     3121 cctggtggca gaaagtgagc gggggatttg cgcgatatta ctgggcgatg atgacgcgac
     3181 actaatcagc gagttacagc agatgtttcc cgctgccgac agcgcgcctg ctgatctgac
     3241 gtttcagcaa catgtgcgtg aagtgatcgc cagcctcaat caacgcgata cgccgctgac
     3301 gttaccgctg gacattcgcg gcactgcttt tcagcaacaa gtctggcagg cactgcgcac
     3361 gataccttgc ggtgaaaccg tcagttatca gcaactggct aacgccatcg gcaaaccgaa
     3421 agcggtacgg gctgttgcca gcgcctgtgc cgccaacaag ctagctatcg ttataccctg
     3481 tcatcgggta gtacgcggtg atggatcgct ttcaggttac cgctggggcg tgtcgcgtaa
     3541 agcgcaactg ctacgccgcg aagctgaaaa tgaggagagg taatgttgga tctgtttgcc
     3601 gatgctgaac cgtggcaaga accactggcg gctggagcgg tgattttgca ccgttttgct
     3661 tttaacgctg cggagcaact gatccgcgat attaatgacg ttgccagcca gtcgccgttt
     3721 cgccagatgg tcactccagg cggatatacc atgtcggtgg cgatgaccaa ctgtgggcat
     3781 ctgggctgga cgacccatcg gcaaggttat ctctattcgc ccattgatcc gcaaacaaat
     3841 aaaccgtggc ccgccatgcc gcagagtttt catcatttat gtcaacgtgc ggctacggca
     3901 gcgggctatc cagatttcca gccagatgcc tgtttgatca accgctacgt tcctggcgcg
     3961 aaactgtcgc tgcatcagga taaagacgaa ccggatctgc gcgcgccaat tgtttctgtt
     4021 tctctgggct tacccgcgat ttttcaattt ggcggcctga aacgaaatga tccgctcaaa
     4081 cgtttgttgt tggaacatgg cgatgtggtg gtatggggtg gtgagtcgcg gctgttttat
     4141 catggcattc aaccgttgaa agtggggggg catccactca ccaccgactg ccgctacaac
     4201 ctgacattcc gtcaggcagg taaaaaagaa taaaaataag aattattatt gctgtgcgca
     4261 cgaagatgtt taaactgcgg gctgtaattc attgtccggg ttttctgcat ggaacttctt
     4321 gtacttgtct ggcggcagta tcgctggcca tttatcagtg tgatggcgct aagcctcgcc
     4381 agtgcggcat taggcattgg cttaattgct tttatcaatc agcgccttat cgaaacggcg
     4441 gataccagtc tgctggtatt gccggagttt ctggggttat tgctgctgtt gatggcagtc
     4501 actctcggat cgcaactggc gctcaccact ttggggcatc acttcgttta ccgactgcgt
     4561 agtgaattta tcaagcggat tctggatacc catgtcgagc gcattgaaca acttggtagc
     4621 gcctcgttgc tggcggggtt aaccagcgat gtgcgcaata tcaccattgc ttttgtgcgt
     4681 ctgccggaac tggtgcaggg gatcattctc actatcggtt cagcggcgta tttgtggatg
     4741 ctgtcgggca aaatgttgct ggtaacggct atctggatgg cagtcaccat ctggggtggt
     4801 tttatactgg tggcgcgggt gtacaaacat atggcgaccc tgcgtgaaac cgaagataag
     4861 ctgtacacgg attttcaaac cgtactcgaa gggcgcaaag agctgactct gaaccgtgaa
     4921 cgcgccgagt atgtgtttaa caacctctac attcctgatg cgcaagagta tcgccatcat
     4981 attatccgcg cagacacctt ccatcttagt gccgtgaact ggtcaaacat catgatgctg
     5041 ggcgcaatcg gcctggtgtt ctggatggcg aacagtctcg gttgggctga taccaacgtt
     5101 gccgcgacct attcgttgac gttgttattc ctgcgtacgc cgctgctttc ggcggttggc
     5161 gcgttgccga cgctactgac ggcgcaggtg gcgtttaata agctgaacaa attcgcgctc
     5221 gcgcctttca aagcagagtt tccgcgcccg caggcgtttc ccaactggca aacgctggag
     5281 ctgcgtaacg tgacgtttag ctatcaggat aacgcgtttt ccgttggtcc aattaatctc
     5341 accattaaac gtggcgagct gctgtttctg attggtggca acggtagtgg aaaatcgacg
     5401 ctggcgatgt tgctgacggg cttgtaccag ccacaaaatg gcgaaatctt gctggatggc
     5461 aaacctgtca gcggcgaaca accggaagat tatcgaaaac tgttttcggc agtgtttacc
     5521 gatgtctggc tgtttgatca actgctgggg ccggaggggc aacccgctaa cccgcaactg
     5581 gttgagaagt ggctggcgca gctgaaaatg gctcataagc ttgagttaag caacgggcgt
     5641 attgttaacc tgaagttatc aaaagggcag aaaaaacgcg tggcgctgtt gctggcgctg
     5701 gcagaagaac gcgatattat cctgctggat gaatgggcgg cggatcagga tccacacttc
     5761 cgtcgtgagt tttatcaggt gctgctgccg ctgatgcagg agatgggtaa aactattttc
     5821 gccatcagtc atgatgatca ttactttatc cacgccgacc gcctgctgga aatgcgcaac
     5881 gggcaactta gcgagctgac aggcgaagag cgcgatgctg cttcgcgtga tgccgttgcc
     5941 cggacagcat aatattacga cgctgacctg gcggactgct gccgccaggt caatattcta
     6001 aaaatacctt cgtttctaat aaccctataa ttaatcaacg aaattataat gtttctaaaa
     6061 ttagaatata atttataaac attatttaaa tgttgttact taagtgttac cgttgatgcc
     6121 gcgcaaatct cacctgcaat aaagcgacta aaagtaaggc attaacaaga tgaaaaaagt
     6181 gactgccatg ctcttctcga tggccgtggg gcttaatgcc gtttcgatgg cggcaaaagc
     6241 gaaagcgtcc gaggagcagg aaactgatgt actgttgatt ggcggcggca ttatgagcgc
     6301 cacgttgggg acctatttac gcgagctgga gcctgaatgg tcgatgacca tggtggagcg
     6361 cctggaaggt gtcgcgcagg agagttcgaa cggctggaac aacgccggaa ccgggcattc
     6421 tgcactgatg gaactcaatt acaccccgca aaatgccgac ggcagtatca gtattgaaaa
     6481 agcagtcgcc attaacgaag catttcagat ttcccgccag ttctgggcgc accaggtcga
     6541 gcgtggtgtg ctgcgtactc cgcgttcatt tatcaatacc gttccgcata tgagctttgt
     6601 ctggggcgag gataacgtta atttcctgcg cgcccgttac gccgcgttgc aacaaagctc
     6661 gctgtttcgc ggtatgcgtt actctgaaga tcacgcgcag atcaaagagt gggcaccgtt
     6721 agtgatggaa gggcgcgatc cgcaacagaa agtagcggca acacgcacag aaattggcac
     6781 cgatgtgaac tatggcgaga tcacccgcca gttgatcgcc tccttacaga agaaatctaa
     6841 cttctcgctg caactcagca gcgaagtccg cgccctaaag cgtaatgacg ataacagctg
     6901 gaccgtcacc gttgccgatc tgaaaaacgg cactgcacag aacatccgcg ccaaatttgt
     6961 ctttatcggc gcgggcggtg cggcgctgaa actgttacag gaatcgggga ttccggaggc
     7021 gaaagactac gctggtttcc cggtgggcgg acagttcctt gtttcggaaa acccggacgt
     7081 ggttaatcac catctggcga aggtttacgg taaagcatcc gttggcgcac caccgatgtc
     7141 ggttccgcat atcgataccc gtgttcttga cggtaaacgc gtagtgctgt ttgggccatt
     7201 tgccaccttc tcaaccaaat tcctcaaaaa cggttcatta tgggatctga tgagttccac
     7261 caccacctct aacgtgatgc cgatgatgca cgttgggctg gataatttcg atctggtgaa
     7321 atatctggtg agtcaggtga tgttgagtga agaggatcgt tttgaagtgt tgaaagagta
     7381 ctatccgcag gcgaaaaaag aggactggcg tttgtggcaa gcggggcagc gcgtgcagat
     7441 tatcaagcgt gatgccgata aaggtggcgt actgcgtctc ggtactgaag tcgtcagtga
     7501 ccagcaaggg accattgccg cgctcctggg ggcatcgcca ggggcgtcaa ccgccgcgcc
     7561 gattatgttg aatctgctgg aaaaagtatt tggcgatcgc gtttccagcc cgcaatggca
     7621 ggctacgttg aaagcgatcg ttccgtctta tggacgcaag ctgaacggtg atgtagcggc
     7681 aacagaacgc gagttgcagt acaccagcga agtgctgggt ctgaaatacg acaagccgca
     7741 ggcagcagat agtacgccga aaccgcagtt gaaaccgcaa cccgttcaaa aagaagtggc
     7801 ggatattgcg ttgtaatgat gtgccacatc cggcatggtt tgccggatgt ggcgtatgct
     7861 gataagacgc ttcagcgtcg catcaggcaa ccggctcggg cgttagatgt cggatgcggc
     7921 gtaaccgcct tattcggcct acaggttgca gcacttcaaa gcagtttagc gaacaaccgc
     7981 gttgtcaatt ttctcttccg ccttccagac acggtacttc acatccacat tgtctggcgt
     8041 atacaccacg atcggcagct tgctgttgta acgcagcatt ccagcatcgc ccagatacgc
     8101 ggtgacaaat ttcttctctt tcttgccatc cgggcaggcc atcatcgtgg aaaccggcga
     8161 gctaacttta tcaaagacat aataatcata gccccagcct tccagcgttt tgctttccag
     8221 cttcccgccg agacgatgca aattgcagtc gacttccagc gtctgaccga ttaacagttc
     8281 tactttcagg gtagattcat cttcttgcgg agttaactga atcacctgac gcttcatgcc
     8341 tttttcagct tgtggataag gcgcgatttt ttccagtggc tggacgctct ctgccgccca
     8401 ggcggaagtg gtggcgaaag cggcaaacaa tactgcaggt agaatggtct tcattttttt
     8461 gctttccttc ttcatttata ggttttcacc gatgatgtcg ctgaaggtta acatattcat
     8521 cagatttatt aatttgcttt atgaataact taaagaacat
//
LOCUS       DACADF010000177         8510 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.179, whole genome shotgun sequence.
ACCESSION   DACADF010000177 DACADF010000000
VERSION     DACADF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.179"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            208..2586
                     /gene="ppsA"
                     /locus_tag="ISB30_004172"
     CDS             208..2586
                     /gene="ppsA"
                     /locus_tag="ISB30_004172"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAP1991741.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGVDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2643..4343)
                     /gene="fadK"
                     /locus_tag="ISB30_004173"
     CDS             complement(2643..4343)
                     /gene="fadK"
                     /locus_tag="ISB30_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416216.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HAP1991742.1"
                     /translation="MHPTGPHLGPDVLFRESKMKVTLTFNEQRRAAYRQQGLWGDASL
                     ADYWQQTARAMPDKIAVVDNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPG
                     WCEFTVIYLACLKTGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLIL
                     PLQNQLPQLQQIVGVDKLAPATSSLSLSQILADNTPLTTAITTHGDELAAVLFTSGTE
                     GLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARS
                     VLLDIFTPDACLALLEQQRCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIP
                     KKVARECQQRGIKLLSVYGSTESSPHAVVNLDDPLPRFMHTDGYAAAGVEIKVVDDAR
                     KTLPPGCEGEEASRGPNVFMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGR
                     KKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLS
                     LEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTVSGKIQKFLLRKDIMRRLTQDVCEEIE
                     "
     gene            complement(4346..4639)
                     /locus_tag="ISB30_004174"
     CDS             complement(4346..4639)
                     /locus_tag="ISB30_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAP1991743.1"
                     /translation="MSQNATVNVDIKLGVDKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(4636..5925)
                     /locus_tag="ISB30_004175"
     CDS             complement(4636..5925)
                     /locus_tag="ISB30_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP1991744.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASNPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5980..6918)
                     /locus_tag="ISB30_004176"
     CDS             complement(5980..6918)
                     /locus_tag="ISB30_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAP1991745.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGSAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTRDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLNR"
     gene            complement(6938..7702)
                     /locus_tag="ISB30_004177"
     CDS             complement(6938..7702)
                     /locus_tag="ISB30_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAP1991746.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            8018..>8510
                     /locus_tag="ISB30_004178"
     CDS             8018..>8510
                     /locus_tag="ISB30_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP1991747.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYEN"
BASE COUNT         2029 a         2190 c         2204 g         2087 t
ORIGIN      
        1 tcatttttat ggtttcgttt ataccgatgg tttatgtgga aattgtcgaa gagagcagat
       61 ttgcgcaacg ctgggatcag tcttaaaaag taaaaaaata tatttgcttg aacgattcac
      121 cgtttttttc atctggttaa atatgcaaag ataaatgcgc agaaatgtgt ttctcaaacc
      181 gttcatttat cacaaaagga ttgttcgatg tccaacaatg gctcgtcacc gctggtgctt
      241 tggtataacc aactcggcat gaatgatgta gacagggttg ggggcaaaaa tgcctccctg
      301 ggtgaaatga ttactaacct ttccggaatg ggtgtttccg ttccgaatgg tttcgccaca
      361 accgccgacg cgtttaacca gtttctggac caaagcggcg taaaccagcg catttatgaa
      421 ctgctggata aaacggatat tgacgatgtt acccagcttg cgaaagcggg cgcgcaaatc
      481 cgccagtgga ttatcgacac tcccttccag cctgagctgg aaaacgccat ccgcgaagcc
      541 tatgcacagc tttccgccga tgacgaaaac gcttcgtttg cggtgcgttc ctccgctact
      601 gcagaagata tgccggacgc ttcttttgcc ggtcagcagg aaactttcct caacgttcag
      661 ggtgttgacg ccgttctcgt ggcagtgaag catgtattcg cttctctgtt taacgatcgc
      721 gccatctctt atcgtgtgca ccagggttac gaccatcgtg gcgtagcgct ctccgccggt
      781 gttcagcgga tggtgcgctc cgacctcgca tcttctggcg tgatgttctc cattgatacc
      841 gaatccggct ttgaccaggt ggtgtttatc acttccgcat ggggccttgg tgaaatggtc
      901 gtgcagggtg cggttaaccc ggatgagttt tacgtccata aaccgacact tgcggcgaat
      961 cgcccggcta tcgtgcgccg caccatgggg tcgaaaaaaa tccgcatggt ttacgcgccg
     1021 acccaggagc acggcaagca ggttaaaatc gaagacgtac cgcaggaaca gcgtgacatc
     1081 ttctcgctga ccaacgaaga agtgcaggaa ctggcaaaac aggccgtaca aattgagaaa
     1141 cactacggtc gcccgatgga tattgagtgg gcgaaagatg gtcacaccgg caaactgttc
     1201 attgtgcagg cgcgtccgga aaccgtgcgc tcacgcggtc aggtcatgga gcgttatacg
     1261 ctgcattcac agggtaagat tatcgccgaa ggccgtgcta tcggtcatcg catcggcgcg
     1321 ggtccggtga aagttatcca tgacatcagt gaaatgaacc gcatcgaacc tggcgacgtg
     1381 ctggttactg acatgaccga cccggactgg gaaccgatca tgaagaaagc atctgccatc
     1441 gtcaccaatc gtggcggtcg tacctgtcac gcggcgatca tcgctcgtga actcggcatt
     1501 ccggcggtag tgggctgtgg tgatgcaaca gaacggatga aagacggcga gaacgtcact
     1561 gtttcttgtg ccgaaggtga taccggttac gtctatgcgg agttgctgga atttagcgtt
     1621 aaaagctcca gcgtagaaac gatgccggac ctgccgttga aggtgatgat gaacgtcggt
     1681 aacccggacc gtgctttcga cttcgcctgc ctgccgaacg aaggcgtggg ccttgcgcgt
     1741 ctggaattta tcatcaaccg tatgattggc gtccacccac gcgcactgct tgagtttgac
     1801 gatcaggaac cgcagttgca aaacgaaatc cgcgagatga tgaaaggttt tgattctccg
     1861 cgtgaatttt acgttggtcg tctgactgaa gggatcgcga cgctgggtgc cgcgttttat
     1921 ccgaagcgcg tcattgtccg tctctctgat tttaaatcga acgaatatgc caatctggtc
     1981 ggtggtgagc gttacgagcc agatgaagag aacccgatgc tcggcttccg tggcgcgggc
     2041 cgctatgttt ccgacagctt ccgcgactgc ttcgcgctgg agtgtgaagc agtgaaacgt
     2101 gtgcgcaacg acatggggct gactaacgtt gagatcatga tcccgttcgt gcgtaccgtt
     2161 gatcaggcga aagcggtggt tgaggaactg gcgcgtcagg ggctgaaacg tggtgagaac
     2221 gggctgaaaa tcatcatgat gtgtgaaatt ccgtccaacg ccttgctggc agagcagttc
     2281 ctggaatatt tcgacggctt ctcaattggc tcaaacgaca tgacgcagct ggcgctcggt
     2341 ctggatcgtg actccggcgt ggtgtctgaa ctgttcgatg agcgcaacga tgcggtgaaa
     2401 gcactgctgt cgatggcgat tcgtgccgcg aagaaacagg gcaaatatgt cgggatttgc
     2461 ggtcagggtc catccgacca cgaagacttt gctgcatggt tgatggaaga ggggatcgat
     2521 agcctgtctc tgaacccgga caccgtggtg caaacctggt taagcctggc tgaactgaag
     2581 aaataaaata aatacccggc ggcgttttgt cgccgggttt atgtgatccc cgaagatgaa
     2641 acttattcaa tctcttcaca gacatcctga gttaatcgcc gcataatatc tttccttaac
     2701 aaaaactttt gtattttacc tgagacagtg cgcggtagtt tttcgattac cacgatatgt
     2761 tcaggatatt tatattttgc gacccgttta cggctaaaaa aagccaccac ctcttccagc
     2821 gataatgaat gatgcggcgc tttcagcacg acataagcgc atgaacgttc acctaaacgt
     2881 tcatcgggca tcgcaaccac acaggcatcg tgaattttag gatgctgcaa taaaatatct
     2941 tctacttcac ggctgctaat attttcgccg ccgcggacaa taatatcttt cttgcgcccg
     3001 gttattttta tatagccagc ctcatccatg cggcagagat cgccgctgta ataccagcct
     3061 tcttcatcca gcgcacgggc ggttaattca ggttcatcaa aataccccat aaacacattg
     3121 gggccacgcg aggcttcttc accttcgcaa cctggcggta aagtcttgcg tgcgtcatcg
     3181 accactttaa tctctacacc tgcggcagcg taaccgtcgg tgtgcataaa gcgcggcaaa
     3241 ggatcgtcga gattcaccac cgcatgcggc gaactttctg tggaaccata aacacttaat
     3301 aatttaatgc cgcgctgctg gcattctcgc gccacttttt tggggattgt ggtacctccg
     3361 caaagaaaga agcgcagcgc tgaaaggtct gcgggttgtt tctctagtaa attcaaaaga
     3421 tcatagacaa acggcgttgc gccgagcata caggtgcaac gctgctgctc aagcagcgcg
     3481 agacacgcat caggagtgaa aatatctaac aacacgctgc gagcaccaat caaaaaaggt
     3541 gctgttacgc catgcagaaa gcccgttgcg tgaccaagtg gcgcaggcat cataaagaca
     3601 tcctgccagg tcagatttag ccgcgcgcaa taagcccgct cactggcgag aatattgtta
     3661 tgcgttagca tcacaccctt tggcagaccc tcggttccgg aggtaaatag cacagcggct
     3721 aattcatcgc cgtgggtcgt tatcgccgtg gtcagaggag tattgtcggc gagaatctga
     3781 cttaatgaga gggaagaggt ggcgggagcc agtttgtcca cgccgacaat ttgttgtagt
     3841 tgtggaagct gattttgcag cggcaggatt aaatctaccg gacgcgtttg tttaaacaac
     3901 gtcggtgcaa agaacatttt tgcctgacac ttattgagca cccacaccag ttctgcttcc
     3961 cgccaggaag gtaacagcgg cacggaaacc gcaccggttt tcaggcaggc aagatagata
     4021 acggtaaatt cacaccagcc tggcagttga aatgcgatgc gatcgcctga ttcaataccc
     4081 ttcgctaaca tccagtttgc cagacagctc gcggcgtgat cgagcgcgct ataggtgtac
     4141 gttgcaccat gattatcgac cacggcaatt ttgtctggca tcgcacgagc ggtctgctgc
     4201 cagtaatcgg ccagcgaagc atcgccccat aacccttgct gacgatacgc cgcacgacgt
     4261 tgttcgttga acgttaatgt cactttcatt ttggactctc gaaacagaac atcaggcccg
     4321 agatgcgggc ctgtgggatg catgattagc cgtagcgaaa ttcaataccg aaggtgcctg
     4381 cgggatattg ccactgttcg agaatagtgt tgccgcacag cacccgacag gtgccgcact
     4441 ccagacaacc ggcggaatcg aaatgaatgt ttcctgcgtc atcctgctta taaagtccgg
     4501 cagggcaagc tttcattaat ttacggaatt cattgatatc gggatttgcc gccaaaatga
     4561 tatgcgggtg gccctcatca acatggaatt tatcgacgcc taatttgatg tcgacgttaa
     4621 ccgtagcgtt ctggctcata gcgcggttgc tcccttaatg ccatctttca gcaagttgat
     4681 cagcccaatt ttcttcgcgt gtcccatgat cattttgcgt accggctggt ttggtttgcc
     4741 gtcaatggtg aacatatcgt tcatgatgtc ggcgaccatt cgtgggtatt ggctaaacag
     4801 gcgcgggttt tccatcagcg ccgggatctt gcggaaatgc tgcatatcgc gcataacaca
     4861 actttgttcc agctcacgtt tgtattgcgc cagactgctg gcggagaaat ccgcgcgttc
     4921 tttggcggcg atcaccgttg tggcggcagc ctgagctgat gcaatggcta aatccatgcc
     4981 gcgaactgta aaacccaaat tcaggcagaa gcctgcggcg tcaccaacga tcatcacgcc
     5041 atcgttaacc agctgcggca ccattgccag accgccctcc ggcaccatat gcgcggaata
     5101 ttcaagcagt ttgccgccgc taatcagcgg gcgaatagcg gggtgttgtt taaaatcttc
     5161 cagcatttgc ggcacgcttt tttgcgcatg ggcgatatca cccaatccac aaaccagccc
     5221 caacgatacg gaatccttgt tggtatagag gaatccgccg cccatcaggc cgtcagaagg
     5281 ggaaccggca aacagccagg cggcaccttc ctcgcccgta acattaaagc gatcgttgat
     5341 ctgttctggt gtcaggccta taacctcttt aacaccaaca gcgtaatgat gcggattgga
     5401 agcggaaacc attcccagcg agcggccaag catcgagtta acgccatcag ccagaatcac
     5461 cacattcgct tcgagaatat catccccggc ctggacgcca gtgaccttgt ttccttcacg
     5521 aaccagcgca tcgacgcgta cgcccgggat aaactgcgcg cccgcctgct cggcttgttc
     5581 catcaaccac gggtccagac gattacgcaa tacggtataa gacgcgtgtt gcggaacatc
     5641 tggttgctcg cggtgaaaat cgagggtaac ggcgctttct tcggttaaga aggagatttt
     5701 ctcgcgtgtg accttgcgtt ctaccggcgc tgatgctgca aagcctggaa taattgcttc
     5761 aagtgtgtgg gcataaagac gcccgccggt catgttttta catccggcac tgtcgccgcg
     5821 ttctatcacc aggacatcca gcccggctcg cgccatgacc agtgcggcaa cgctaccggc
     5881 aacgcccgca ccgaccacaa tggcatcaaa tttgtcatcc gacatgcgtt atgctccctt
     5941 cccgctgcgt ttacagcggg agatgtggtt gaaagggaat tagcgattca actggctaat
     6001 cagggcaggg acgactttgt agatatcgcc caccagaccg tagtcggcat agttgaagat
     6061 tggcgcattt ttatctttat taatggcgac aatcactttt gcgccgttgc cgccaaccat
     6121 atgctggatc tgcccggaga tccccagcgt caggtagaga tcggatttca gcaacacgcc
     6181 ggagacaccg atataacgtt cacgctccat ccagttctcg ccttcggcaa ttggacgtga
     6241 acagccgact tcagcattca gcactgccgc cagttcgtgg accattttca ggtcatcctg
     6301 cgccgccaga ccacgaccga cgccaaccac acgttttgct ttgctcaggt ccacgctgct
     6361 tgccgctttc gcacggcgtt cgcgacagag aatttcatga cgcggggcaa cataaggtac
     6421 cgtttctgtc gggcactgat gagagatatc acgggtgcac ggttcctgaa caccgggtgc
     6481 aagggtaatg atcgccagcg ggctgttgat cttttcctga gcgaacgcta acccgccata
     6541 catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc accgccgtgg catcgttcac
     6601 cagcgccgca ttcagttgca cacttaaccg tgcggccaga gctttaccac gtttcgtcgc
     6661 ggccaacagc aacatagcgg ggtgtttatc tttcagcagg gcagcaatgc tttcggcgta
     6721 attttcagta cgttgcagcg cgtcgttttg ctcaagaaca taaatgcatt ttggcccata
     6781 aggcataact gcctgcgcct gctcggtatt ttgtacaatg gcatacacct gttggcccca
     6841 ttgctgagcg ctgccaaaca gttcagcata acgttcagga ttatcgctaa agacccagac
     6901 gctgtttaat tgactcatcg tttcttctcc gtaggcttca gttcagggct ttcttcagat
     6961 gttccgccag ctcggcaatg gcctccggcg aatcgttatc gagaatgatg tgcttacgtt
     7021 ctgtttgcgg cggtacgcga atgccggtca gttcagcaag tggtgcgctc tggctccagc
     7081 caatatcact tgcctgccac tgatttaccg gttttttacc cgcgccgaga atggctttca
     7141 tcgaaggaat acgtggcacg ttaatatcgg aggtgacgca gagcacggct ggaacagaga
     7201 gttcaataac ttcaacatca tcttcaagcg tgcgttcaat caccagtgta ttgccctgac
     7261 gctgaatagc actcactgca ttaatcaccg gaagttgcag aatttctccg accagcaagc
     7321 caacctgctg ggcataaagg tcgccggaac cttcaccaaa gatcagtaaa tcgaagccga
     7381 tcttttcaac tgctgtcgcc agcgcctttg cggtatcgag aggcagtgca tgctcaagtt
     7441 gtgcatcctg caccatatac aggctgtgcg gcccgcggga tagcacgtct ttgcgcactt
     7501 tcgagttctg caacaaagag ccgccgacgg tcagcgcggc tatctcatcg tcatctgttg
     7561 caagctggct tgcagcttca atggcattga gatcgaactg gctgattttg gcgtcggcat
     7621 tgtcgaaatt cagggtgtat tctggagtga caacaatgtc ctgttcttca ggcaccagct
     7681 taaagcaggt tattattttc atgatttctc ctgtgagtcg catacggaac catctccgat
     7741 ggaaagtaaa aatggcctga aaatagtgtg cgatagcggt gcggggtttt cgacggagca
     7801 agctgttttc cttgccaaca atcttgcaaa aaaaatgcga tcgctaaaac aaaagtgcaa
     7861 tttgcggcaa tatagttatt ccatgaccgt actatcttgc attcagtgta aataatcttg
     7921 tttagctctc gctaaacgcg gttgtataac cgcaatacac gccgtatttt atccggcata
     7981 tattgttttt gcttgctcat ggaataggga ggttgtcatg tatcaacgct gttttgataa
     8041 tgccagtgaa acgctgtttg tcgccggtaa aacgccacgg ctttcacgtt tcgcatttag
     8101 cgatgatcca aaatgggagt ctggacatca cgttcatgac aatgaaaccg agctgattta
     8161 cgtcaagaaa ggggtagcaa ggtttaccat cgattcttcg ttatatgtcg cgcatgccga
     8221 tgacattgtg gtgatagaac gtggcaggtt gcatgcggtg gcctctgacg ttaacgatcc
     8281 ggcaacgacg tgtacctgtg cgctgtacgg ctttcagttt cagggggctg aggaaaatca
     8341 gctactgcaa ccgcattctt gcccggtaat tgccgcagga cagggaaaag aagtcattaa
     8401 aaccttattt aatgagctaa gtgtgatttt gccgcaaagt aaaaatagcc aaacatcttc
     8461 gttatgggac gcatttgcct atacgttagc aattctttac tacgaaaact
//
LOCUS       DACADF010000178         8476 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.180, whole genome shotgun sequence.
ACCESSION   DACADF010000178 DACADF010000000
VERSION     DACADF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.180"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(131..1102)
                     /locus_tag="ISB30_004179"
     CDS             complement(131..1102)
                     /locus_tag="ISB30_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HAP1991748.1"
                     /translation="MKRAPVIPKHTLNTQPVGDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDSLDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(1102..2268)
                     /gene="sopA"
                     /locus_tag="ISB30_004180"
     CDS             complement(1102..2268)
                     /gene="sopA"
                     /locus_tag="ISB30_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HAP1991749.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(2856..3611)
                     /gene="repE"
                     /locus_tag="ISB30_004181"
     CDS             complement(2856..3611)
                     /gene="repE"
                     /locus_tag="ISB30_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HAP1991750.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(4385..5191)
                     /locus_tag="ISB30_004182"
     CDS             complement(4385..5191)
                     /locus_tag="ISB30_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HAP1991751.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(5192..5497)
                     /gene="ccdB"
                     /locus_tag="ISB30_004183"
     CDS             complement(5192..5497)
                     /gene="ccdB"
                     /locus_tag="ISB30_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAP1991752.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(5499..5717)
                     /gene="ccdA"
                     /locus_tag="ISB30_004184"
     CDS             complement(5499..5717)
                     /gene="ccdA"
                     /locus_tag="ISB30_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAP1991753.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            6425..7420
                     /locus_tag="ISB30_004185"
     CDS             6425..7420
                     /locus_tag="ISB30_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP1991754.1"
                     /translation="MATTVIAAFNEFMKDTVNLKKADTDDARASRDWLIGKMNDFEKD
                     DKFPVSFPAIHIAFGSFARRTKIRPLDDIDLMFGLTGQGATYTILSDRITVTSSGEGS
                     RLHSYRHSGADTVCSVRILNAFKNRLQDIAQYAQADIRRNQEAVTLKLVSKDWNFDIV
                     PCFITSEDAFGRTYYLIPDGNGHWKFTDPRKDRDRVTTINVQNNGNVLNVIRAVKYWQ
                     RRPTMPSMSSYLLETLILDYYAGRTSCSSFVDMELEALFRHLGQSVRYSVNDPKGIQG
                     DINSLSAEARKAISDRCYLDAQKVSEARWFENNKEYEKSINKWRDVFGPFFPVYG"
     gene            7424..8356
                     /locus_tag="ISB30_004186"
     CDS             7424..8356
                     /locus_tag="ISB30_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991755.1"
                     /translation="MNAIYTNQSTRENMDLLYAQARVYDRVKNWNRLNFLFSIIVPLL
                     LSFVTVYNRSREFVDSELLSSLLGLYGLLVLTFNIVISGHISALRRKAASIQEMYDCR
                     VLGIRRNELKVEEIPRDDIIREAAYFRDSPEKARKRFGEEGWYVSKVYDAPQAVMALL
                     CHGKNLGWDKSLREVLHVFYLSAFIVSPVAMLVYGIVMKSGLNEILFYVVFTLPVIRY
                     FLLQFLDNRSSMKRSEKLKKYVEKELSGIRVSGRAEEEQLAYTLRNIQDEMFTYRASC
                     PPVPNGIQLIMKPKNERIYVDYFETNLKELHLQK"
BASE COUNT         2322 a         2071 c         1931 g         2152 t
ORIGIN      
        1 ctgattatca gaccgacgat acaagtggaa cagtgggccc agagagaata ttcaggccag
       61 ttatgctttc tggcctgtaa caaaggacat taagtaaaga cagataaacg tagactaaaa
      121 cgtggtcgca tcagggtgct ggcttttcaa gttccttaag aatggcctca attttctcta
      181 tacactcagt tggaacacga gacctgtcca ggttaagcac cattttatcg cccttataca
      241 atactgtcgc tccaggagca aactgatgtc gtgagcttaa actagttctt gatgcagatg
      301 acgttttaag cacagaagtt aaaagagtga taacttcttc agcttcaaat atcaccccag
      361 cttttttctg ctcatgaagg ttagatgcct gctgcttaag taattcctct ttatctgtaa
      421 aggctttttg aagtgcatca cctgaccggg cagatagttc accggggtga gaaaaaagag
      481 caacaactga tttaggcaat ttggctgtgt tgatacagcg ggtaataatc ttacgtgaaa
      541 tattttccgc atcagccagc gcagaaatat ttccagcaaa ttcattctgc aatcggcttg
      601 cataacgctg accacgttca taagcacttg ttgggcgata atcgttaccc aatctggata
      661 atgcagccat ctgctcatca tccagctcgc caaccagaac acgataatca ctttcggtaa
      721 gtgcagcagc tttacgacgg cgactcccat cggcaatttc tatgacacca gatactcttc
      781 gaccgaacgc cggtgtctgt tgaccagtca gtagaaaaga agggatgaga tcatccagtg
      841 agtcctcagt aagcagctcc tggtcacgtt cattacctga ccatacccga gaggtcttct
      901 caacactatc accccggagc acttcaagag taaacttcac atcccgacca catacaggca
      961 aagtaatggc attaccgcga gccattactc ctacgcgcgc aattaacgaa tccaccatcg
     1021 gggcagctgg tgtcgataac gaagtatctc caaccggttg agtattgagc gtatgttttg
     1081 gaataacagg cgcacgcttc attatctaat ctcccagcgt ggtttaatca gacgatcgaa
     1141 aatttcattg cagacaggtt cccaaataga aagagcattt ctccaggcac cagttgaaga
     1201 gcgttgatca atggcctgtt caaaaacagt tctcatccgg atctgacctt taccaacttc
     1261 atccgtttca cgtacaacat tttttagaac catgcttccc caggcatccc gaatttgctc
     1321 ctccatccac ggggactgag agccattact attgctgtat ttggtaagca aaatacgtac
     1381 atcaggctcg aaccctttaa gatcaacgtt cttgagcaga tcacgaagca tatcgaaaaa
     1441 ctgcagtgcg gaggtgtagt caaacaactc agcaggcgtg ggaacaatca gcacatcagc
     1501 agcacatacg acattaatcg tgccgatacc caggttaggc gcgctgtcaa taactatgac
     1561 atcatagtca tgagcaacag tttcaatggc cagtcggagc atcaggtgtg gatcggtggg
     1621 cagtttacct tcatcaaatt tgcccattaa ctcagtttca atacggtgca gagccagaca
     1681 ggaaggaata atgtcaagcc caggccagca agtgggcttt attgcataag tgacatcgtc
     1741 cttttcccca agatagaaag gcaggagagt gtcttctgca tgaatatgaa gatctggtac
     1801 ccatccgtga tacattgagg ctgttccctg ggggtcgtta ccttccacga gcaaaacacg
     1861 tagccccttc agagccagat cctgagcaag atgaacagaa actgaggttt tgtaaacgcc
     1921 acctttatgg gcagcaaccc cgatcaccgg tggaaatacg tcttcagcac gtcgcaatcg
     1981 cgtaccaaac acatcacgca tatgattaat ttgttcaatt gtataaccaa cacgttgctc
     2041 aacccgtcct cgaatttcca tatccgggtg cggtagtcgc cctgctttct cggcatctct
     2101 gatagcctga gaagaaaccc caactaaatc cgctgcttca cctattctcc agcgccgggt
     2161 tattttcctc gcttccgggc tgtcatcatt aaactgtgca atggcgatag ccttcgtcat
     2221 ttcatgacca gcgtttatgc actggttaag tgtttccatg agtttcattc tgaacatcct
     2281 ttaatcattg ctttgcgttt ttttattaaa tcttgcaatt tactgcaaag caacaacaaa
     2341 atcgcaaagt catcaaaaaa ccgcaaagtt gtttaaaata agagcaacac tacaaaagga
     2401 gataagaaga gcacatacct cagtcactta ttatcactag cgctcgccgc agccgtgtaa
     2461 ccgagcatag cgagcgaact ggcgaggaag caaagaagaa ctgttctgtc agatagctct
     2521 tacgctcagc gcaagaagaa atatccaccg tgggaaaaac tccaggtaga ggtacacacg
     2581 cggatagcca attcagagta ataaactgtg ataatcaacc ctcatcaatg atgacgaact
     2641 aacccccgat atcaggtcac atgacgaagg gaaagagaag gaaatcaact gtgacaaact
     2701 gccctcaaat ttggcttcct taaaaattac agttcaaaaa gtatgagaaa atccatgcag
     2761 gctgaaggaa acagcaaaac tgtgacaaat taccctcagt aggtcagaac aaatgtgacg
     2821 aaccaccctc aaatctgtga cagataaccc tcagactatc ctgtcgtcat ggaagtgata
     2881 tcgcggaagg aaaatacgat atgagtcgtc tggcggcctt tctttttctc aatgtatgag
     2941 aggcgcattg gagttctgct gttgatctca ttaacacaga cctgcaggaa gcggcggcgg
     3001 aagtcaggca tacgctggta actttgaggc agctggtaac gctctatgat ccagtcgatt
     3061 ttcagagaga cgatgcctga gccatccggc ttacgatact gacacaggga ttcgtataaa
     3121 cgcatggcat acggattggt gatttctttt gtttcactaa gccgaaactg cgtaaaccgg
     3181 ttctgtaacc cgataaagaa gggaatgaga tatgggttga tatgtacact gtaaagccct
     3241 ctggatggac tgtgcgcacg tttgataaac cagggaaaag attcatagcc tttttcatcg
     3301 ccggcatcct cttcagggcg ataaaaaacc acttccttcc ccgcgaaact cttcaatgcc
     3361 tgccgtatat ccttactggc ttccgcagag gtcaatccga atatttcagc atatttagca
     3421 acatggatct cgcagatacc gtcatgttcc tgtagggtgc catcagattt tctgatctgg
     3481 tcaacgaaca gatacagcat acgtttttga tcccgggaga gactatatgc cgcctcagtg
     3541 aggtcgtttg actggacgat tcgcgggcta tttttacgtt tcttgtgatt gataaccgct
     3601 gtttccgcca tgacagatcc atgtgaagtg tgacaagttt ttagattgtc acactaaata
     3661 aaaaagagtc aataagcagg gataactttg tgaaaaaaca gcttcttctg agggcaattt
     3721 gtcacagggt taagggcaat ttgtcacaga caggactgtc atttgagggt gatttgtcac
     3781 actgaaaggg caatttgtca caacaccttc tctagaacca gcatggataa aggcctacaa
     3841 ggcgctctaa aaaagaagat ctaaaaacta taaaaaaaat aattataaaa atatccccgt
     3901 ggataagtgg ataaccccaa gggaagtttt ttcaggcatc gtgtgtaagc agaatatata
     3961 agtgctgttc cctggtgctt cctcgctcac tcgagggctt cgccctgtcg ctcgactgcg
     4021 gcgagcacta ctggctgtaa aaggacagac cacatcatgg ttctgtgttc attaggttgt
     4081 tctgtccatt gctgacataa tccgctccac ttcaacgtaa caccacacga agatttctat
     4141 tgttcctgaa ggcatattca aatcgttttc gttaccgctt gcaggcatca tgacagaaca
     4201 ctacttccta taaacgctac acaggctcct gagattaata atgcggatct ctacgataat
     4261 gggagatttt cccgactgtt tcgttcgctt ctcagtggat aacagccagc ttctctgttt
     4321 aacagacaaa aacagcatat ccactcagtt ccacatttcc atataaaggc caaggcattt
     4381 attctcagga taattgtttc agcatcgcaa ccgcatcaga ctccggcatc gcaaactgca
     4441 cccggtgccg ggcagccaca tccagcgcaa aaaccttcgt gtagacttcc gttgaactga
     4501 tggacttatg tcccatcagg ctttgcagaa ctttcagcgg tataccggca tacagcatgt
     4561 gcatcgcata ggaatggcgg aacgtatgtg gtgtgaccgg aacagagaac gtcacaccgt
     4621 cagcagcagc ggcggcaacc gcctccccaa tccaggtcct gaccgttctg tccgtcactt
     4681 cccagatccg cgctttctct gtccttcctg tgcgacggtt acgccgctcc atgggtattt
     4741 tcagtgttgc caccatcgtc tgcagctggc tgacgtacca ggagtcagag agcggaacca
     4801 gccggtgagt ctgctgaccg gcgggcattc tccccgccgt cctggcagct ttttcggtcc
     4861 gttgtttcag ggtcgcaagc tgcacaaacg gatacggagg cgcaagcgaa aaatcccccc
     4921 gcgtcagcgc cagtgcttca ttaatgcgtg ctccggtgtt ccacagtgtg gccagcagca
     4981 tcttgcggtg cagatccggg acgtaatgga gcagggcact cacttccgga gccagcagat
     5041 attttggcag ttcatcatgg accatcgaca tctggcgaag tgccagagct gccggataat
     5101 caatggcaac aggcagcgat gcaggctgcc cggcagaata cactgccggt accatgactg
     5161 cagactggct gtgtataacg gagcctgaca tttatattcc ccagaacatc aggttaatgg
     5221 cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg ataacggaga
     5281 ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg atatgcacca
     5341 ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc agggggatca
     5401 ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac agacgataac
     5461 ggctctctct tttataggtg taaaccttaa actgcatttc accagtccct gttctcgtca
     5521 gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc ctgattttcc
     5581 gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt gcttaccaga
     5641 ccggagatat tgacatcata tgccttgagc aactgatagc tgtcgctgtc aactgtcact
     5701 gtaatacgct gcttcatagc acacctcttt ttgacatact tcgggtatac atatcagtat
     5761 atattcttat gccgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta
     5821 agccttatgt attttacctt tcgttatgtt aaccaataaa aattaaaatc tgacttataa
     5881 aaacaaagcg taattaccgc attcccgttt cgtatggatc cgactttatt tgaataaacg
     5941 actttccgga agtgtacaac gctgagaaaa tcatcatatc gctgttttcc gcccctgcgc
     6001 ggcgcatcac agtccagaga tacaacacaa catatagtgc cattggcaac tgtatgaggc
     6061 actacatgct gtgctgacgg gatgtaacgc tgtgcggagg gagaggaaga tccgtaactt
     6121 acttttttcg gatctctgga gaggggaaga aggatttgtg ctgatgatgc aatactaatg
     6181 atatattttg aacttaattg cattattgct tatctggcat taataactga aatactttgc
     6241 ataattatca atgaaagaac gaggctcata tttcccatgt aaattatatg cccttgcaga
     6301 gagcgagacg gtagtgaact tgcggcacta ataataaact gtaccgactc ttcccgggca
     6361 ctggaatgtt cagatgtacg acagagcccc gcgccttttg caataaataa ttaaaggact
     6421 gaacatggcg accacagtaa tagctgcctt taacgaattt atgaaagata ccgtgaatct
     6481 caaaaaggca gataccgatg acgcgcgtgc aagtcgcgac tggcttatcg gtaagatgaa
     6541 tgattttgag aaggacgata aatttccggt gagttttcca gcgatccata ttgcctttgg
     6601 ctcctttgcc agaagaacca aaatccgtcc gcttgatgat atcgatctga tgtttggctt
     6661 aaccgggcaa ggtgccacct ataccatcct gagtgaccgg ataactgtaa cctcctccgg
     6721 agaggggtca cgtctgcaca gttaccgcca ttccggagct gacaccgtct gctccgtcag
     6781 aattctgaat gcctttaaaa accgcctgca ggacattgcc cagtacgccc aggctgatat
     6841 caggcgtaac caggaagctg tcacgctgaa actggtcagc aaggactgga attttgacat
     6901 cgtcccctgc tttattacca gcgaagatgc ctttggacgg acttactacc tgatccccga
     6961 tggcaacggc cactggaagt ttaccgaccc ccgtaaagac agggacagag tcactactat
     7021 caatgtgcag aataacggca atgtgctcaa tgtcatcaga gcagtcaaat actggcagcg
     7081 gcgaccgacg atgccatcca tgagttccta cctgctggaa acgctgattc tggactatta
     7141 cgctggcagg acatcctgct catcgtttgt tgacatggag ctggaagcgc ttttccgcca
     7201 ccttggtcaa tccgtgcggt actctgtcaa cgatcccaaa ggcatacagg gtgatatcaa
     7261 ctcactttca gccgaggcac gtaaggcgat atcggacaga tgttatctcg atgcgcaaaa
     7321 ggtatcggag gcgagatggt ttgaaaataa taaagagtat gaaaaatcca tcaataaatg
     7381 gcgcgatgtg ttcgggccat tctttcctgt ttacgggtga atcatgaatg ccatttacac
     7441 aaaccagtcg accagagaaa acatggattt actgtatgcg caggcccgtg tctacgacag
     7501 ggtaaaaaac tggaacagac tgaattttct cttcagcatc atcgtaccgc tcctgctttc
     7561 tttcgttacc gtatacaacc ggagccggga atttgtcgat tcagagttgt tgtcttcgct
     7621 gttggggtta tatggactcc tggtgcttac ctttaacatt gttatcagtg gacacatctc
     7681 ggctctgcgc aggaaggcag cctcaatcca ggaaatgtac gactgccggg tacttggcat
     7741 ccggcgtaat gaactgaagg tggaggagat cccacgggat gacatcatcc gggaagctgc
     7801 atattttaga gacagcccgg aaaaagcacg aaagcgattt ggcgaagagg ggtggtacgt
     7861 cagtaaggtg tatgacgcgc cgcaggccgt aatggcgctc ctttgccacg gaaaaaatct
     7921 cggctgggac aaatcgctca gggaggttct gcacgtattt tatctttccg cttttattgt
     7981 ctcgcctgtc gcgatgcttg tttacggcat cgtcatgaaa tccgggctga atgagatcct
     8041 cttctatgtg gtgttcacac tgccggtcat ccgctatttc ttgcttcagt ttctggataa
     8101 tcgcagcagt atgaaaagaa gcgagaagct gaaaaagtac gttgagaagg aattatccgg
     8161 cataagggtg tccggaaggg cagaagaaga gcaactggct tatacgctcc ggaatatcca
     8221 agatgagatg tttacctacc gggcctcatg tccgcccgta cccaacggta ttcagctgat
     8281 aatgaagccc aaaaatgaac gaatttatgt tgattacttt gaaacaaatc tgaaggagct
     8341 gcaccttcag aagtaatccc tccttagctc cggtgtagga tttacgtatg ccggagcaca
     8401 tccgataaac aattcttggt tgcctgagtg tcagcgtaaa cccatagtca ccttcgcctc
     8461 agacggttag taacac
//
LOCUS       DACADF010000179         8468 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.181, whole genome shotgun sequence.
ACCESSION   DACADF010000179 DACADF010000000
VERSION     DACADF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.181"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            74..964
                     /gene="alsR"
                     /locus_tag="ISB30_004187"
                     /gene_synonym="rpiR"
     CDS             74..964
                     /gene="alsR"
                     /locus_tag="ISB30_004187"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HAP1991756.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            1023..1958
                     /gene="alsB"
                     /locus_tag="ISB30_004188"
     CDS             1023..1958
                     /gene="alsB"
                     /locus_tag="ISB30_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HAP1991757.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATETFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            2086..3618
                     /gene="alsA"
                     /locus_tag="ISB30_004189"
     CDS             2086..3618
                     /gene="alsA"
                     /locus_tag="ISB30_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HAP1991758.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCRPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            3597..4577
                     /gene="alsC"
                     /locus_tag="ISB30_004190"
     CDS             3597..4577
                     /gene="alsC"
                     /locus_tag="ISB30_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HAP1991759.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSS
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            4588..5283
                     /gene="rpe"
                     /locus_tag="ISB30_004191"
     CDS             4588..5283
                     /gene="rpe"
                     /locus_tag="ISB30_004191"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP1991760.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     RERKDLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            5267..6196
                     /gene="alsK"
                     /locus_tag="ISB30_004192"
     CDS             5267..6196
                     /gene="alsK"
                     /locus_tag="ISB30_004192"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HAP1991761.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            6469..8454
                     /gene="yjcS"
                     /locus_tag="ISB30_004193"
     CDS             6469..8454
                     /gene="yjcS"
                     /locus_tag="ISB30_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HAP1991762.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMNNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         2207 a         2054 c         2155 g         2052 t
ORIGIN      
        1 aagttgccag catcttttct gttcttgctg tggtgatata gtggcgtctt caattcaagg
       61 acaagagaac gtgatgagcc agtcagagtt tgattcagcg cttccgaacg gtatcgggtt
      121 agcgccttac ctgcgaatga agcaggaagg aatgacagaa aacgaaagcc gcatcgtgga
      181 gtggttactc aaacccggta acctgagttg tgcacccgca attaaagatg tcgcagaagc
      241 tctggcggta tctgaagcga tgatcgttaa ggtatcaaag ctgctggggt ttagcggctt
      301 tcgtaactta cgcagtgcgc tggaagatta tttttctcag tcagaacaag tattgccgtc
      361 cgagttggct tttgatgaag cgccgcagga tgtggtgaat aaggtattta acatcacttt
      421 acgcaccatt atggaaggtc agtcgatcgt caacgttgat gagatccacc gtgccgcccg
      481 ctttttctat caggccagac agcgggattt gtacggtgcc ggaggatcaa atgcaatctg
      541 tgctgatgta cagcacaagt ttttacgcat tggcgtacgc tgccaggcct atccggacgc
      601 tcacatcatg atgatgtctg cttcgttgtt acaggaagga gatgtggtgc tggtggtgac
      661 ccattccggg cgaaccagtg atgtaaaagc agctgtagaa ctggcaaaaa agaacggggc
      721 aaagattatt tgtataacgc atagctacca ttcaccgata gcgaagctgg ccgattatat
      781 tatttgctca ccggccccgg aaacaccgtt attaggtcgt aatgcctcgg caagaatatt
      841 acaactaact ttgctggacg cgttttttgt ctctgtcgcc cagctcaaca ttgaacaagc
      901 taatattaat atgcaaaaaa ccggcgcaat tgttgatttc ttctcaccag gcgcgctgaa
      961 ataagaaaaa ggtaattccg ccatcgcgga gttacctatc cccttaccgt gagaacatga
     1021 ttatgaataa atatctgaaa tatttcagcg gcacactcgt gggcttaatg ttgtcaacca
     1081 gcgcttttgc tgccgccgaa tatgccgtcg tattgaaaac actctccaac ccattttggg
     1141 tagatatgaa aaaaggcatt gaagatgaag caaaaacgct gggcgtcagc gttgatattt
     1201 ttgcctctcc ttcagaaggc gattttcaat ctcaattgca gttatttgaa gatctcagta
     1261 ataaaaatta caaaggtatc gccttcgcac cattatcctc agtgaacctg gtcatgcctg
     1321 tcgcccgcgc atggaaaaaa ggcatttatc tggtcaatct cgatgaaaaa atcgacatgg
     1381 ataatctgaa aaaagcaggc ggtaatgtgg aaggttttgt caccaccgat aacgttgccg
     1441 tcggggcgaa aggcgcgtca ttcattattg acaagctggg tgccgaaggg ggtgaagttg
     1501 caattattga aggtaaagcc ggtaacgcct ccggtgaagc gcgtcgtaat ggtgccaccg
     1561 aaaccttcaa aaaagcaagc cagatcaagc ttgtcgccag ccagcctgcc gactgggacc
     1621 gcattaaagc actggatgtc gccactaacg tgttgcaacg taatccgaat attaaagcga
     1681 tctattgcgc gaatgacacg atggcaatgg gtgttgctca ggctgtcgca aacgccggaa
     1741 aaacgggaaa agtgctggtc gtcggtacag atggcattcc ggaagcccgc aaaatggtgg
     1801 aagccggaca aatgaccgcg acggttgccc agaacccggc ggatatcggc gctacgggtc
     1861 taaagctgat ggttgacgct gaaaaatccg gcaaggttat cccgctggat aaagcaccgg
     1921 aatttaaact ggtcgattca atcctggtca ctcaataaaa ctaaatgatg ccggatagtg
     1981 atgctaatga agtgtcttat ccggccaaca aaataacctg aacgtagacc tgataagtgt
     2041 agcccatcag gcaataatac gatatttcgt gcagagaggt tatttatggc cacgccatat
     2101 atatcgatgg cggggatcgg caagtccttt ggtccggttc acgcattaaa gtcggttaat
     2161 ttaacggttt atcctggtga aatacatgca ttactaggag aaaatggcgc gggtaaatcc
     2221 acgctaatga aagtgttatc cggaatacat gagccaacca aaggcaccat taccattaat
     2281 aacattaact ataacaaact ggatcataaa ttagcggcac aactcggtat cgggattatt
     2341 tatcaggaac tcagcgttat tgatgaatta accgtactgg aaaatttata tattggtcgt
     2401 catctgacga aaaaaatctg cggtgtcaat attatcgact ggcgagaaat gcgtgtccgc
     2461 gccgcaatga tgttattacg cgtgggcttg aaagttgatt tagatgagaa agtggcgaac
     2521 ttatctatca gccacaagca gatgctggaa attgccaaaa cgctgatgct cgacgccaaa
     2581 gtcatcatca tggatgaacc cacctcctca ctcaccaata aagaggtgga ctatctgttt
     2641 ctgatcatga atcagttgcg taaggagggt acggctatcg tctatatctc gcataaattg
     2701 gcggaaattc gccgtatttg cgaccgctat acggtgatga aagacggcag cagcgtttgc
     2761 agcggcatgg tgagcgatgt gtcaaatgac gatatcgtcc gtctgatggt aggccgcgaa
     2821 ctgcaaaacc gttttaacgc gatgaaggag aatgtcagca accttgcgca cgatacggtt
     2881 tttgaggtgc gcaacgtcac cagccgtgac agaaaaaagg tccgggatat ctcatttagc
     2941 gtctgccggg gagaaatatt aggctttgcc ggactggtcg gttccggacg tactgaactg
     3001 atgaactgcc tgtttggcgt tgataaacgc gctggcggag aaatccgtct taatggcaaa
     3061 gatatctctc cacgttctcc cctggatgcc gtgaaaaaag ggatggctta catcactgaa
     3121 agccgccggg ataacggctt tttccccaac ttttctatcg ctcagaacat ggctatcagc
     3181 cgcagtctga aagacggcgg ctataaaggc gcgatggggt tgtttcatga agttgacgag
     3241 caacgtaccg ctgaaaatca acgcgaactg ctggcgctga aatgtcattc ggtaaaccag
     3301 aacatcactg aactctccgg cggtaaccag cagaaagtcc tgatctccaa atggctgtgc
     3361 tgtcgcccgg aagtgatcat tttcgatgaa cctacccgtg gcatcgacgt tggcgcgaaa
     3421 gccgaaattt acaaagtgat gcgccaactg gcggacgacg gaaaagtcat cctgatggtg
     3481 tcatctgaac tacctgaaat tatcaccgtc tgcgaccgca tcgccgtgtt ctgcgaagga
     3541 cgactgacgc aaatcctgac gaatcgcgat gacatgagcg aagaggagat tatggcatgg
     3601 gctttaccac aagagtaaaa agcgaagcga gcgagaagaa accgttcaac tttgcgctgt
     3661 tctgggataa atacggtact ttttttatcc tggcgatcat cgtcgccatt tttggttcgc
     3721 tgtcgtcaga atattttctg accaccaata atattaccca gatttttgtg caaagctccg
     3781 tgacggtact gatcggcatg ggggaattct tcgctatcct ggtcgccggt atcgaccttt
     3841 cggttggcgc gattctggcg ctttccggta tggtgaccgc taaactgatg ttggcaggta
     3901 ttgatccgtt tctcgcggcg ctaattggcg gtgtactggt tggcggcgca ctgggggcga
     3961 tcaacggctg cctggtcaac tggacgggac tacacccgtt cattatcacc cttggcacca
     4021 acgctatttt ccgtgggatc acgctggtga tctccgatgc caactcggta tacggcttct
     4081 catttgactt cgtgaacttc tttgccgcca gcgtaattgg gatacctgtc cccgttatct
     4141 tctcgctaat tgtcgcgctc atcctttggt ttctgacaac gcgtatgcgg ctcgggcgca
     4201 acatctacgc actgggcggc aacaaaaact cggcgttcta ttccgggatt gacgtgaaat
     4261 tccacatcct ggtggtgttt atcatctccg gtgtttgtgc aggtctggca ggcgtcgtct
     4321 caactgcacg actcggtgcc gcagaaccgc ttgccggtat gggttttgaa acctatgcca
     4381 ttgccagcgc catcattggc ggcaccagtt tcttcggcgg caaggggcgc attttctctg
     4441 tggtgattgg cgggttgatc atcggcacca tcaacaacgg tctgaatatt ttgcaggtac
     4501 aaacctatta ccaactggtg gtgatgggcg gattaattat cgcggctgtc gcccttgacc
     4561 gtcttatcag taagtaagga attgaacatg aaaatctccc cctcgttaat gtgtatggat
     4621 ctgctgaaat ttaaagaaca gatcgaattt atcgacagcc atgccgatta ctttcacatc
     4681 gatatcatgg acggccactt tgtgccgaat ctgacgctct caccgttctt cgtaagccag
     4741 gtaaaaaaac tggcgagtaa accgctcgac tgccatctga tggtaacgcg tccgcaggat
     4801 tacattgctc aactggcacg tgcgggagct gatttcatca ctttgcatcc ggaaactatc
     4861 aacggtcagg cattccgcct gattgatgaa atccgccgtc atggcatgaa agtggggctg
     4921 attcttaatc cggaaacgcc ggttgaggcc atgaaatact atatccataa ggctgataaa
     4981 attacggtca tgactgtcga tcccggtttt gccggacaac ccttcattcc tgaaatgctg
     5041 gataaagttg ccgaactgaa ggcatggcgt gaacgaaaag atctggagta cgaaattgag
     5101 gtggacggtt cctgcaacca ggcaacttac gaaaaactaa tggcggcagg ggcggatgtc
     5161 tttatcgtcg gcacttccgg cctgtttaat catgcggaaa atatcgacga agcatggaga
     5221 attatgaccg cgcagattct ggctgcaaaa agcgaggtac agcctcatgc aaaaacagca
     5281 taacgtcgta gcgggcgtgg atatgggggc aacgcatatc cgcttttgtc tgcggacagc
     5341 agaaggtgaa acgctacact gcgaaaaaaa gcggaccgca gaagttattg ctcccgacct
     5401 ggtgtcgggt atcggcgaaa tgattgacga gcaactcagg cgctttaacg ctcgctgtca
     5461 tggtctggtg atgggatttc cggcgctggt cagtaaagat aaacgcacca ttatttctac
     5521 gcctaacctg ccgttaacag cggcagattt atatgatctc gccgataagc tcgaaaatac
     5581 gctgaattgt ccggttgagt tttcccgcga cgttaacctg caactttcct gggacgtagt
     5641 agaaaaccgc cttacgcaac aactggttct ggcggcctat ctcggtacgg ggatggggtt
     5701 cgcagtgtgg atgaacggtg cgccgtggac gggtgcacac ggtgtggcag gcgaactggg
     5761 ccatatcccc ctgggagata tgacccaaca ctgcgcgtgt ggcaattctg ggtgcctgga
     5821 aaccaattgc tctggaatgg cgctaagacg ctggtacgaa caacagcccc gaaattaccc
     5881 attgagcgat cttttcgtcc atgcggaaaa cgcccctttc gtccagagtc tgcttgaaaa
     5941 cgcggcacga gccattgcca ccagcattaa tctgttcgat cccgatgcgg tgattctggg
     6001 cggtggcgtg atggatatgc ccgccttccc acgcgagact ctcattgcca tgacccaaaa
     6061 gtacctgcgc cgtccactgc cgtatcaggt cgtgcgcttt attgccgcct catcttctga
     6121 ctttaatggc gctcagggtg cagcaatatt ggcgcatcaa cgttttttgc cacagtcctg
     6181 tgctaaagtc ccatgagtaa agcgttggca cagtagcgca atgtcccgct gtgccaacga
     6241 caggtacaat atatataata attacataaa acagcccata ttattcccag caatgatagt
     6301 tctactgtaa ttttccctct tcagcacaaa tgcctaataa aatggctcgt tccgctcaca
     6361 cttccactta ttaaagtcgc aaattcccaa ttatgataac gccagcttta aatgacgtat
     6421 tttgcatgga ttacatattt acgacataaa tgataaatgg agatttatat gaataactct
     6481 cggttattcc gtttgagcag gattgttatt gcgttcactg ccgccagcgg catgatgata
     6541 aataccgctt acgcaacaga tgaggcgaaa gccgccactc aatataccca acaggttaat
     6601 cagaattacg ccaaatcatt accgtttagc gatcgccagg attttgacga tgctcagcgt
     6661 ggatttatcg ctccgctgct ggatgaaggt attctgcgtg atgcgaacgg taaaatttac
     6721 taccgcgcga atgattacaa atttgatatt aatgccgctg cgccggaaac cgtaaacccc
     6781 agcctgtggc gtcagtcgca gatcaacggc atttcaggcc tgttcaaagt caccgataaa
     6841 atgtatcagg tgcgcggcca ggatatctct aacattacgt tcgttgaggg cgagaaaggc
     6901 attattgtta ttgacccact ggtgacgcca cctgccgcaa aagccgcact tgacctttac
     6961 ttccagcatc gtccgcaaaa accgattgtt gccgttatct acactcacag ccacgccgac
     7021 cactacggcg gcgtgaaagg tattatctct gaagccgatg ttaaatccgg caaagttcag
     7081 gtgattgctc cagctggctt tatggacgaa gccatcagcg aaaacgtgct ggcgggtaac
     7141 atcatgagcc gccgtgcgct ctactcttac ggtctgttac tgccgcacaa cgcgcaaggc
     7201 aacgtgggca acggcctcgg cgtgacgctg gcaacgggcg acccgagcat tattgcaccg
     7261 actaaaacta tcgtcagaac tggcgagaag atgattatcg acggcctgga gtttgacttc
     7321 ctgatgaccc caggtagcga agcgccagcg gaaatgcact tctatattcc ggccctgaaa
     7381 gccctgtgta ccgccgagaa tgccacgcat accctgcaca acttctacac cctgcgcggc
     7441 gcgaaaaccc gcgacaccag caagtggacc gagtatctga acgaaacatt ggatatgtgg
     7501 ggtaacgacg cggaagtact gtttatgccg cacacctggc cggtctgggg caataagcat
     7561 atcaatgatt atattggtaa atatcgcgat actatcaagt acattcacga ccagaccctg
     7621 cacctggcga accagggcta caccatgaat gaaatcggcg acatgattaa gctgccgcct
     7681 gcgcttgcca ataactgggc cagccgcggc tattacggtt ctgtcagcca caacgcccgc
     7741 gcggtatata acttctatct tggctattac gacggtaacc cggctaacct gcatccgtat
     7801 ggtcaggtgg agatgggtaa acgttacgtg caggcgctgg gcggttctgc ccgcgtcatc
     7861 aacctggcgc aagaagcgaa caagcaaggt gattatcgct ggtcggcaga actgctgaaa
     7921 caggtgattg ccgccaaccc gggtgaccag gtcgcgaaga atctgcaagc gaataacttt
     7981 gaacagctgg gctatcaggc cgagtccgcc acatggcgcg gtttctacct gaccggcgcg
     8041 aaagagctgc gcgaaggggt gcataagttc agccacggca ccaccggttc cccggacacc
     8101 attcgtggga tgtcggtcga aatgctgttc gactttatgt ccgttcgcct cgatagcgcg
     8161 aaagccgcgg gtaaaaatat cagcctgaac ttcaatatga acaacggcga taacctcaac
     8221 ctgacgctga acgatagcgt gcttaactac cgtaaaacac tgcaaccgca agctgacgcc
     8281 tctttctaca tcagccgtga agatctgcac gccgtgctga ccggacaggc aaaaatggct
     8341 gatctggtga aagcgaagaa agccaaaatt attggcaatg gtgcgaaact ggaagaaatt
     8401 atcgcctgtc tggataattt cgatttgtgg gtgaatatcg taaccccaaa ttaatatcac
     8461 tcgctttt
//
LOCUS       DACADF010000180         8394 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.182, whole genome shotgun sequence.
ACCESSION   DACADF010000180 DACADF010000000
VERSION     DACADF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.182"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(114..1181)
                     /gene="purK"
                     /locus_tag="ISB30_004194"
     CDS             complement(114..1181)
                     /gene="purK"
                     /locus_tag="ISB30_004194"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP1991763.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARQPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRAAETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQLQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSHLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(1178..1687)
                     /gene="purE"
                     /locus_tag="ISB30_004195"
     CDS             complement(1178..1687)
                     /gene="purE"
                     /locus_tag="ISB30_004195"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP1991764.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(1783..2112)
                     /locus_tag="ISB30_004196"
     CDS             complement(1783..2112)
                     /locus_tag="ISB30_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991765.1"
                     /translation="MQRCNGFLISGIALTAGAPLCISWFGLVGFYFGALSLVAAAVIS
                     WIVYFWLIYLVLTRPHNQRFVERYILIVGICFLPWSIVLIWAFFASSARAHNQHAEQI
                     KNSTTDG"
     gene            complement(2223..2945)
                     /gene="lpxH"
                     /locus_tag="ISB30_004197"
     CDS             complement(2223..2945)
                     /gene="lpxH"
                     /locus_tag="ISB30_004197"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAP1991766.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(2948..3442)
                     /gene="ppiB"
                     /locus_tag="ISB30_004198"
     CDS             complement(2948..3442)
                     /gene="ppiB"
                     /locus_tag="ISB30_004198"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAP1991767.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3616..5001
                     /gene="cysS"
                     /locus_tag="ISB30_004199"
     CDS             3616..5001
                     /gene="cysS"
                     /locus_tag="ISB30_004199"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP1991768.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(5037..5558)
                     /locus_tag="ISB30_004200"
     CDS             complement(5037..5558)
                     /locus_tag="ISB30_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP1991769.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKAIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKWVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(5666..5878)
                     /gene="ybcJ"
                     /locus_tag="ISB30_004201"
     CDS             complement(5666..5878)
                     /gene="ybcJ"
                     /locus_tag="ISB30_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAP1991770.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGLVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(5880..6746)
                     /gene="folD"
                     /locus_tag="ISB30_004202"
     CDS             complement(5880..6746)
                     /gene="folD"
                     /locus_tag="ISB30_004202"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAP1991771.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            7017..7093
                     /locus_tag="ISB30_004203"
     tRNA            7017..7093
                     /locus_tag="ISB30_004203"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7051..7053,aa:Arg,seq:tct)
     gene            complement(7101..7409)
                     /locus_tag="ISB30_004204"
     CDS             complement(7101..7409)
                     /locus_tag="ISB30_004204"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1991772.1"
                     /translation="MPALRKMRIDSKTAWLSACRRAGVENFRFHDLWHTRASRLIPSG
                     VPLSVLQEMGGWESREMVRRYAHLAPNHLTEHARKIDDIFGDNVPLWNYRRNKEGVTD
                     "
     gene            complement(7429..7728)
                     /locus_tag="ISB30_004205"
     CDS             complement(7429..7728)
                     /locus_tag="ISB30_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1991773.1"
                     /translation="MRWNGTRDLQKNQAKRLIDESPELLKSVVKFALVTVLRKSNIIN
                     LEWQQIDMQRQVARVDPEDSKSNRAIGVALNDTASKVLRDQTGKHHKCGCTYQGG"
     gene            7786..8091
                     /locus_tag="ISB30_004206"
     CDS             7786..8091
                     /locus_tag="ISB30_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP1991774.1"
                     /translation="MVVFNSDEASWHLVEDHLGKTVYDVASGDALFISELGPLPENVT
                     WLSPAGEFQKWNGTSWIKDTEEETSLLEAWKMYRVLLNRVDTSTAPDIEWPVNPVRE"
     gene            complement(8146..>8394)
                     /locus_tag="ISB30_004207"
     CDS             complement(8146..>8394)
                     /locus_tag="ISB30_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HAP1991775.1"
                     /translation="VIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIF
                     SNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSASGKK"
BASE COUNT         2007 a         2220 c         2179 g         1988 t
ORIGIN      
        1 gcacaccatg cctgatgcga tgctggtgcg tcttatcagg tctacaggcg gtgttggaac
       61 ggtaggccgg ataaggcgtt cacgccgcat ccggcaatca aatgagcacc aggttaactg
      121 aacttactct gcgcccacat cacgccgctg gcatactccg gtggcagcag cgggattaag
      181 gcttccagcg tcgcggtcag atgcgatgtg tcgctgtcgg tcaaatttaa atgccccact
      241 ttacggcccg gacggacttc tttgtcgtac cagtgcagat gcacaagcgg cagtttcagc
      301 cagtcataat tcagatcgct accaatcaga ttgatcatca ccgacggatt attcactact
      361 ggttgcggta acggcagatc ggtaattgcc cgcagatgta gctcaaactg gctgatgctg
      421 gcaccgtttt gcgtccagtg cccactgtta tgcacacgcg gagccagttc gttgatcagc
      481 agaccttgcg gcgtgacaaa acactccatc gccatcacgc ccacatagcc cagctcctgc
      541 ataatcgccg acaacatctc ttcagcttgc gcctgcagct gtgcgttggc ctgcgggaaa
      601 gcgacgctgg tgcgcaaaat gccgtcctga tgcaggttat gcgtcagcgg ataaaacacg
      661 gtgctgccat caaaaccgcg cgcgccaacc agcgacactt cacctgagaa gtttatgccc
      721 tgctcgacaa tacattcgcc gtagcattca gccggtaact gttcggtttc agcggcgcgt
      781 aaacgccatt gaccgcgtcc gtcatagcca ccagtgcggc gcttaacaat cgccagctca
      841 cctaaacgat caaacaccgc aggccactcg ctgcgttcgg caagtaactg ccacggtgcg
      901 gtcggcaggt ggagcttatc gaaaagctgc ttctgagtca gacggtcggc aataatcggg
      961 aacacatcgc ggttcacaaa ggccggctgg cgcgccagct cgcgggttaa tgcagtttcc
     1021 ggccagcgtt cgatctcagc ggtaatcacg ctttgttgaa aaggcaccgc cgccggttca
     1081 gcgtccagcc cgaccggcca gacagcaatg cctaacggtt cgcctgcctg acgcagcata
     1141 cggcctaact gcccgttacc gaggacgcaa acctgtttca tgccgcacct cgcgggtccg
     1201 ggttttccag cacttcatca gtttgtgctt tgcgccagtc attcagacgc tggtgcagtt
     1261 ctttatcatg agtcgcaaga atttgtgctg ccagtaacgc cgcgtttgcc gcgccagctt
     1321 taccaatcgc cagcgtaccc accggaatgc cgcgcggcat ttgtacgatg gagtagaggc
     1381 tatcgacacc gctcagtgcg gcgctctgta ctggcacgcc cagcaccggc accagcgttt
     1441 tggcggcaat catgccaggc aggtgcgctg cgccgcctgc gcccgcaata atcacctgat
     1501 aaccgttctc ttcggcgctt tcggcgaagc tgaacagttt atcgggggtg cggtgagcag
     1561 aaaccacttc aacgtggtgc gggacattca ggatttcgaa gatttcggcg gcgaactgca
     1621 tggtagccca gtcgcttttg gaccccatca cgatggcgac acgcgccgga ttattgcggg
     1681 aagacatgcg tcttaaaact cctgtggtgc acaactctcg gctttagagg gcacagagaa
     1741 tagcacggaa agagtgctag gaaaacggtt gcgtaggcgg aattaaccgt cagttgttga
     1801 gtttttaatt tgttctgcgt gttgattatg cgctctggct gaactggcaa agaatgccca
     1861 tatcagcaca atgctccacg gtaaaaagca tataccgaca atcaatatat atctttctac
     1921 aaagcgttgg ttatgggggc gggtgagtac taaataaatg agccagaaat agactatcca
     1981 ggaaatgacc gccgcagcta ccagtgacaa tgcgccaaaa tagaagccga caagaccgaa
     2041 ccagctaata cacagtggcg ctcctgccgt aagggcgatg ccagaaatca aaaatccgtt
     2101 gcagcgttgc ataataatat tccctgttgt tatttcactc agagattatt accttgctga
     2161 ttttaaaaaa gatatacagg ttgcgttagt tcggtgcttt ttcagaatct gtccattcgt
     2221 ggtcagaaag gaaaatggat tagctcaacg tcatccgccg tgactttcac catcgaacct
     2281 tctgtatgcc aggcacccag taccacgcga aaagcaggtt gctgattggc gataagttca
     2341 tgcaccgccg ggcgatgggt atgcccgtgg atcagccatt gcacttgatg tttttccatc
     2401 gcactgacca ccgcgttttg gttaacgtcc atgatcgcca gcgatttact gctgttggct
     2461 tctttactgt tcgcgcgcat tcgcgcggca atgcgtttgc gcacaaacaa cggcagggcg
     2521 aggaatagcg tttgcagcca gggtttgtgg actttggcgc gaaaagcctg ataacccgcg
     2581 tcatcggtgc acagcgtgtc gccatgcata atcaacaccc gacgaccata aagttcgagc
     2641 accttttctt ccggcaataa cgtcatgccg ctttcacggg caaagcgttt gccgagcaga
     2701 aaatcacggt tgccatgaat gaaataacag ggaacgccgg aatcggatac cgctttgatc
     2761 gctgcggcca tctggtgatg aagtgggttg ggatcgtcgt cgccgatcca ggcttcaaac
     2821 agatcgccaa gaatatacag cgcatcggcc ttgcgggcct cccctgctaa aaaacgcaga
     2881 aaaccggcgg tgatcgccgg ttcttccacg cagagatgaa gatctgcaat aaagagtgtc
     2941 gccacgatta ctcgctaacg gtcacgcttt caatgataac gtcttctttt ggcacgtcct
     3001 ggtgcatacc gctacgaccg gttgccacac ctttgatttt gtctaccacg tccatgcctt
     3061 caaccacttc agcaaatacg cagtagcccc aaccttgcag gctttcgcca gagaagttca
     3121 ggaagtcgtt atcaaccacg ttgatgaaga actgggcagt tgcagagtgc ggtgcctgag
     3181 tacgggccat tgccagcgta ccacgggtat ttttcagacc gttgttcgct tcgtttttga
     3241 tcgggtcttt ggtggctttt tgtttcatgc ccggttcaaa accgccgccc tgaatcataa
     3301 agccgttgat aacacggtgg aaaatggtgt tgttgtaaaa accttcgcgg cagtagtcca
     3361 ggaagttttt aactgtttca ggtgctttat cgtcaaaagt tttgatgaca atatcgccgt
     3421 gattggtgtg gaaagtaacc atttttgcat cctgttccgt ttgattggtg cttcaacccg
     3481 gttcgggtca tatatagggt ggtgttatag cataaccgca cgatcggatc atctcgcaat
     3541 gtatgctgat tcgcgcggga aatatgggta ttatacgcaa ctcaattacc cacacatgtc
     3601 taaacggaat cttcgatgct aaaaatcttc aatactctga cacgccaaaa agaggaattt
     3661 aagcctattc acgccgggga agtcggcatg tacgtgtgtg gaatcaccgt ttacgatctc
     3721 tgtcatatcg gtcacgggcg tacctttgtt gcctttgacg tggttgcgcg ctatctgcgt
     3781 ttcctcggct ataagctgaa gtatgtgcgc aacattactg atatcgacga caaaatcatc
     3841 aaacgcgcca atgaaaatgg cgaaagcttt gtggcgctgg tggatcgcat gatcgccgaa
     3901 atgcacaaag attttgatgc tttgaacatt ctgcgcccgg atatggagcc gcgcgcgacg
     3961 caccatatcg ccgaaattat tgaactcact gaacaactga tcgctaaagg tcacgcttat
     4021 gtggctgaca acggcgacgt gatgttcgac gtcccgaccg atccgacata tggcgtgctg
     4081 tcgcgtcagg atctcgatca gctgcaggca ggcgcgcgcg ttgacgtggt cgacgacaaa
     4141 cgcaacccga tggacttcgt tctgtggaaa atgtcgaaag agggcgaacc tagctggccg
     4201 tctccgtggg gcgcgggccg tcctggctgg cacattgaat gttcggcaat gaactgcaag
     4261 cagctgggta accactttga tatccacggc ggcggttctg acctgatgtt cccgcaccac
     4321 gagaacgaaa ttgcccagtc cacctgtgcg cacgatggcc agtatgtgaa ttactggatg
     4381 cactccggta tggtgatggt tgaccgcgag aagatgtcta aatcgctggg taacttcttc
     4441 accgtgcgtg atgtgctgaa atactacgat gcggaaactg tgcgttactt cctgatgtcg
     4501 ggtcactatc gcagtcagtt gaactacagc gaagagaacc tgaaacaggc gcgtgcggcg
     4561 ctggaacgtc tctacactgc gctgcgcggc acagacaaaa ccgttgcgcc tgccggtggc
     4621 gaagcgtttg aagcgcgctt tatcgaggcg atgaacgacg atttcaacac tccggaagcc
     4681 tattccgtac tgtttgatat ggcgcgtgaa gtgaaccgtc tgaaagcaga agatatggca
     4741 gcggcgaatg caatggcgtc tcacctgcgt aaactttctg ccgtattggg cctgctggag
     4801 caagaaccgg aagcgttcct gcaaagcggt gcgcaggcag acgacagcga agtggctgag
     4861 attgaagcgt taattcaaca gcgtctggat gcccgtaaag cgaaagactg ggcggcggca
     4921 gatgcggcgc gtgaccgtct taacgagatg gggatcgtgc tggaagatgg cccgcaaggg
     4981 accacctggc gtcgtaagta attgcgctat tgccggatgc gagttttcgc atccggttat
     5041 cgtctgcgcc accacaacat tcccatcagt aacatccccg gcaaccacac ccacatcaat
     5101 tcagaaataa tcacctgatg cccgtacggc gtggtgtaac gagacaatgc aaacggcgcg
     5161 actttaatca cctgccaggg agcgaaaaag cgttcatctg accacggcca cagccagcca
     5221 acccatttac cgccagtggt taccgaatcc agcaagctgt gcgatagcaa cgagacggtt
     5281 aaaaacagcc agcagcgaat cagcccagcc ctgaaccatc gacgtccaat cagcacacat
     5341 aacagcggga caacaaacgc aaacaccagc gaatgggtaa acccgcgatg accaaaaaca
     5401 tttccgtaag caacgccaaa tttaaacgac aatacatcgg catcgggcag catcgccagg
     5461 atgattccgg caaataacag acgcggaggg atggctttcg aacccaaccc taaaccaatg
     5521 cataggggaa cggcggcgtg cgtaataacg gttggcatga tggtcgcttc ggcaaaatgt
     5581 cgatgctatc agcatggatg aacggggcgt agagggcaaa agtctgaaaa gagaaccggc
     5641 ctgttgatac aggccgggaa agggatcagg caacaacctg tacgctgtga cctgcaaaac
     5701 tcactgtctg accggcgacg attttgcagc gtttgcgcgt ttcaaccgca ccgtcgactt
     5761 tcaccaggcc ttcggctatc gcgattttcg cctgcgcgcc gctttcgctc cagccttcca
     5821 gtttcagcaa gtcgcacagc tcaacgtgcg gatgtttacc taaagaaaat gtcgccatgt
     5881 tactcaccct gtggatcatg atattcaacg cacgcctgta gcgtgttttc aatcagcgtg
     5941 gcaaccgtca tcgggccaac gccgcccgga acaggcgtaa tgtatgaggc gcgtttagcc
     6001 gcgtcttcaa acacgacgtc gcccacaact ttgccatttt ccagacggtt gatgccgaca
     6061 tcaatcacaa ttgcgccttc tttgatccag tcaccaggaa taaagcccgg cttgccgacg
     6121 gcaacgatca acaaatcggc attttctacg tggtgacgca gatttttagt gaaacggtga
     6181 gtcacggtgg tggtgcaacc tgccagcagc agttccatgc tcattgggcg accgacgata
     6241 ttcgatgcgc caatcaccac ggcgttaagg ccgaaggtat caatgttgta acgctcaagc
     6301 agcgtgacga taccgcgcgg ggtgcaggga cgcagacgtg gcgcgcgctg gcacagacga
     6361 ccgacgttgt aaggatggaa accgtccacg tctttgtccg gatgaatacg ttccagcact
     6421 ttgacgttat caatacccgc cggtaacggc agttgaacca gaatgccatc aatggtgttg
     6481 tcggcattca gcgcatcgat aagctccagc agctccgctt cgctggtggt ttccgggagg
     6541 tcataagagc gggagacgaa cccgacttct tcacaagcct tgcgtttgct tgcgacataa
     6601 atttgcgatg cagggttgct acccaccagc acaacggcca gtcctggcgc ccgcagtccg
     6661 gctgcaatac gcgcctgaac tttttgagca acttcagagc gcacctgctg cgcaatcgtt
     6721 ttaccgtcaa taatctttgc tgccatcaga gagaggattc catctgttac gtaggtcgaa
     6781 ggggatgcgc ctattttgtc agaagcgtgg cgcgctgtca gttttcgttt tctgaaaagt
     6841 aaaaccttct gtacctggct gattcacacc tatcatgata accgtcgttg gtttgccgcc
     6901 atagtgagct acatctgatg cggtcgtaca cttgttcagc aactggttca aaagccattg
     6961 actcagcaag ggttgaccgt ataattcacg cgattacagc acaccgtggt atcaatgcgc
     7021 ccttagctca gttggataga gcaacgacct tctaagtcgt gggccgcagg ttcgaatcct
     7081 gcagggcgcg ccattaaaat tcaatcagtt acgccttctt tatttcttct ataattccag
     7141 agtgggacat tatcaccaaa aatgtcgtct attttcctcg cgtgctcagt caaatgatta
     7201 ggcgcaaggt gagcatatct acgaaccatt tctctggact cccatccgcc catttcctga
     7261 agcactgata atgggacgcc tgacggaatc aacctgcttg cccgggtatg ccagaggtca
     7321 tggaaacgga aattttcaac tcctgcacga cgacaagctg atagccatgc tgtcttgctg
     7381 tcaatgcgca tcttcctgag cgcaggcatt gatgttccat ctgctcgctt agccgccctg
     7441 gtatgtacac ccacatttgt gatgcttgcc tgtttgatca cgcaacactt tagaggcggt
     7501 atcgttcagc gccacaccaa tggcgcgatt tgatttgcta tcttctggat ccaccctggc
     7561 aacttgtcgc tgcatgtcaa tctgttgcca ctccagattt atgatgttcg actttctcag
     7621 aacagttacc agcgcaaatt tgacgacaga tttcagtagt tcggggctct catcaatcag
     7681 gcgttttgcc tgattctttt gtaagtctct tgtgccattc cacctcatac aggtttgcct
     7741 gcaaacagta ccgatattgc accgccagat attccggctg gcttcgtggt tgttttcaac
     7801 agtgatgagg catcgtggca tctcgttgaa gaccatctgg gtaaaacggt ttatgacgtg
     7861 gcatcagggg acgcgttatt tatttctgaa ctcggtccgt taccggaaaa tgtcacctgg
     7921 ttatccccgg caggggagtt tcagaagtgg aacggcacat catggataaa ggatacggag
     7981 gaagaaacct cgttgctgga agcctggaag atgtatcggg tgttgctgaa ccgagttgat
     8041 acatcaactg cccctgatat tgagtggcct gtgaaccctg tcagagagta atcattggga
     8101 ttatgccgca gcacgtcgtg cgcaagaacg tgctgcgtct ggatgctatt ttttccctga
     8161 agcggaaaac attactacag tacctttaac cttggtttta acattctcga aatgctctga
     8221 gagtatatgt gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt
     8281 gtaaatgcgc atcagttttt gaccgaagct attgtgcaca acgccgtcac caagaattgt
     8341 ggctccgtat agagttccat cgtcagttaa ggcctgcgcc gcattgcgta ttac
//
LOCUS       DACADF010000181         8353 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.183, whole genome shotgun sequence.
ACCESSION   DACADF010000181 DACADF010000000
VERSION     DACADF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.183"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            622..852
                     /locus_tag="ISB30_004208"
     CDS             622..852
                     /locus_tag="ISB30_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991776.1"
                     /translation="MQLLCDNNHSALTVQEFVMKKTFTFSLLAIVFASLLSACVPHHH
                     HRHSDGPRGPAPSGKPMPPSNGGPNHGSHGRY"
     gene            complement(889..2817)
                     /gene="dhaR"
                     /locus_tag="ISB30_004209"
     CDS             complement(889..2817)
                     /gene="dhaR"
                     /locus_tag="ISB30_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAP1991777.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSV
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRG"
     gene            3036..4106
                     /gene="dhaK"
                     /locus_tag="ISB30_004210"
     CDS             3036..4106
                     /gene="dhaK"
                     /locus_tag="ISB30_004210"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP1991778.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRLWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            4117..4749
                     /gene="dhaL"
                     /locus_tag="ISB30_004211"
     CDS             4117..4749
                     /gene="dhaL"
                     /locus_tag="ISB30_004211"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAP1991779.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFGKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLSVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            4760..6178
                     /gene="dhaM"
                     /locus_tag="ISB30_004212"
     CDS             4760..6178
                     /gene="dhaM"
                     /locus_tag="ISB30_004212"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAP1991780.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGTPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            6498..8195
                     /locus_tag="ISB30_004213"
     CDS             6498..8195
                     /locus_tag="ISB30_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAP1991781.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAIGDNAMANQYETLANARQKGIEKYLWNDQQSWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
BASE COUNT         2164 a         2129 c         2004 g         2056 t
ORIGIN      
        1 attatgcact taagctaaat ttaccgtttt tggggctagc ccaacatcga gggcgcataa
       61 gctttagccc acttttactt tcctgttttc tttagtaaca gaaggttaac tatcaaatat
      121 ttttgttggg ctatgtgggc taacagtttt catatttacg tgttttttta ttcttctccg
      181 gtttccgttt cagataccgg agtaaacaac agcgtataag cccagggacg tgcatgcttg
      241 aggtgtctca gccgtggcat acgtatcagc catccctcca cgctggtgga ctttttgagc
      301 attccagcct catgaaggat tctgccggca gtatccttgc tgagaggagt agaacctacg
      361 cccttcgcat tacgaatgcg agctaactcc aaaatgagta cgaacatgag tacaaaaaaa
      421 atatatcatt aaatgtagta taaatttcaa tcagatacag caaaaattac aattcaacgt
      481 ctaattccgc taatactact ctggcggtta ataacgagcc attatgtgag ttgcacgtaa
      541 tggctttttt acgcctgcta cgcgtcttct ttcaattctg agttaccaaa actcataatc
      601 agactcctga caatattaac aatgcagctt ttatgtgata acaatcacag tgcattaacc
      661 gttcaagagt ttgtcatgaa aaaaacattt accttcagcc tgttagcaat tgtctttgca
      721 agcctgttga gtgcttgtgt tccccaccat catcaccgcc acagcgatgg ccctcgtggt
      781 cctgcgccct ccggtaagcc aatgccacct tcaaatggtg gaccaaatca cggcagtcat
      841 ggtcgctatt aattagtaat tcatagcggg aaagtacata ctctcccgct atccacggcg
      901 cttaaactgc cctgcatcaa tgccatgttg cttcattttc cgccacagcg tagtgcggcc
      961 gattccaagt aaagccgaca tttcctgaat gcgaccgcct gtgacttggg ctgcgttaat
     1021 aattgcctct ttttcaactt ccgcaaatga cagactggtg gaaaggcggg tggcgctgac
     1081 atcatctgtc gcctgctcgg taaacagatg ttccggcaaa tcactgacgc gaatgcgccc
     1141 attatcacta ctcagagcaa gattctcgat gacgctgtaa agttcaaaat cgttgccagg
     1201 ccatgcacag gaaaccaggc gagcgagggc atcgtcatca attttcagcc gcgtagagaa
     1261 gcgtttttca aggctgcgta atttgttatt caccagcgcc ggaatgctgc cacgccgcat
     1321 acgcagtggc gggatggtaa tttcaaatgc atgcagcgcg taatacagct ggcgactaaa
     1381 acgattttgt tccaccagca ttgcgaggtc cgcggtcgtt gtggcaatca ctttgacatc
     1441 aatgggtatt aaacgccgcg catccagtcg cgtgataaca ccctgcttga taacctgaag
     1501 caaagcagac tgtaactcca ccgccagata ttcaatcttt tcaagaaaca gcgtgccgcc
     1561 gtgcgccagt tccagccgac tcagacggcc attttcatta tccgtgcgat cgccaccaat
     1621 aaattcttcc gccagcgccg catcaccata taactcacaa ttgacggcga tataaggccc
     1681 cgctgcacgc tcgctttcat tatgaattgc ctgacttagc agtgccttgc ccacgccctc
     1741 ttctccacaa agcaggacag gaaagctact gcgcgccgcc tggcgaccaa aatgaatcaa
     1801 gcggcgggtt tgcggatcgt cctgtggcat atgagcgaag gtatggctga cttttcctaa
     1861 ttgactggtc attaactgcc gcatctgttc cacaggatgg agcaataaaa taaagctggt
     1921 tccctgcgtt tctattatcg gtttaagggt tatcaccgca tcaataaact gatgctgact
     1981 ttcaaaggtt gcttctacgt gtttgagcgg atgtgcctgt tttattgctt gttgcaatac
     2041 ggcgggtaac gttaagagtt cagtgattgc ccttccctga cttgccgtcg cgtcaaggcg
     2101 caagactcgc gccgcctggg cgttgataaa ttgcaaatta ccctgctcgt cccagctaat
     2161 cacgccatca tccatacttt ctaacagggc attaagttga tttaaatgac ggttagtttc
     2221 agcgagcaaa ctgtccgtca gcagtaaatt tccgacctcg cgtgcgattg ccaacgtcaa
     2281 cggcaaatca gctgcggtag tttgctcaac cggacacgcc agcgctattg ttcccgtcaa
     2341 tcggcccttg ctgtcaaaca acggcgttgc gcaaaaggcc cagttccaga gtgcctgttt
     2401 gaaatgttga tcggccatcg ttttcacggc ctgaccagag atagccgcta acgatagcgc
     2461 acaagtacca ataattccct cggcgcaata cgtgccgtca ttgaatccca gtacacttag
     2521 ctgctgcaag gtttgcggat cgccattacg gctgagaatg caggcggttt catcgaggat
     2581 aagcaacgca cactctcgcg gtgccatata ttcccaggca tcttccagcg cagcctgccc
     2641 gagcgtcagc atcgctttct tacgccgata aatagaagca aatgtcacgc cctgggcctg
     2701 gtgtggtacg ttccatgtct cccgtttcat cagcttattg catctctccc aggaggttgc
     2761 aattaaggga gatatgcccc gaccatcgtt gttaaaagcg ccactcatat cccgcatccc
     2821 ttatgacaaa aggtgttccg gctcgcaatt ttaagtatta atacacgtaa aagtttcaat
     2881 atggaacact cattaaagca tatgtttcac tatgaaacat aatttgggct aattttcttt
     2941 tcccttgcct gatgcacaat ggattcatcg gcagtcagtg gtcgccgtgt cgttgaacat
     3001 catccatgcc ctaccgtaat tgctggagca aaataatgaa aaaattgatc aatgatgtgc
     3061 aagacgtact ggacgaacaa ctggcaggac tagcgaaagc gcatccatcg ctgacactgc
     3121 atcaggatcc ggtgtatgtc acccgagctg atgcccccgt tgcaggaaaa gtcgccctgc
     3181 tgtcgggtgg cggcagcgga cacgagccga tgcattgtgg ttatatcggt caggggatgc
     3241 tttcgggggc ctgtccgggc gaaattttca cctcaccgac gcccgataaa atctttgaat
     3301 gcgccatgca aattgatggc ggcgaaggtg tactgttgat tatcaaaaat tacaccggcg
     3361 atattcttaa cttcgaaaca gcaaccgagt tactgcacga tagcggcgta aaagtgacca
     3421 ctgtggtcat tgatgacgac gttgcagtaa aagacagtct ttataccgcc gggcgacgcg
     3481 gcgttgccaa caccgtatta attgaaaaac tcgtaggtgc agcggcggag cgtggcgact
     3541 cactggacgc ctgtgcggaa ctggggcgta agttgaataa tcaaggccac tcaataggta
     3601 tcgctctcgg tgcctgtacc gttcctgccg cgggcaaacc ttcttttacc ctggcggata
     3661 atgagatgga gtttggcgtc ggcattcatg gtgagccagg tattgaccgc cgccccttct
     3721 cttcccttga tcaaaccgtc gatgaaatgt tcgacaccct gctggaaaat ggctcatacc
     3781 atcgcacttt gcgtttatgg gattatcaac aaggcagctg gcaggaagaa ccacaaacca
     3841 aacaaccgct ccagtctggc gatcgggtga ttgcgctggt taacaatctt ggcgcaactc
     3901 cgctttctga gctgtacggc gtctataacc gcctgaccac acgttgccag caagcgggat
     3961 tgactatcga acgtaattta attggcgcgt actgcacctc actggatatg accggtttct
     4021 caatcacctt actgaaagtt gatgacgaaa cgctggcact ctgggacgcc ccggtccaca
     4081 ccccggccct taactggggt aaataaggag aaagcaatgt cactgagcag aactcaaatt
     4141 gttaactggc tcactcgttg tggcgatatt ttcagcaccg agagcgagta tcttactgga
     4201 ctggatcgcg agattggcga cgctgaccac gggctaaata tgaaccgagg ctttggcaaa
     4261 gtggtggaaa aactccctgc tatcgcagat aaagatatcg gtttcattct caagaatacc
     4321 ggcatgacgc tactttccag cgtcggaggt gccagtggtc cgctgttcgg taccttcttt
     4381 atccgcgccg cacagacaac ccaggcacgg caaagcctga cgctggaaga gctttatcag
     4441 atgttccgcg atggcgcgga cggcgtaatc agtcgcggga aagccgaacc tggcgataaa
     4501 accatgtgtg atgtgtgggt gccggtggtg gaatcgttac gtcagtcctg cgagcaaaat
     4561 ctctctgttc cggcggcgct cgacgctgcc agtagcatcg ccgaatccgc tgcacaaagt
     4621 acgattacga tgcaagcccg caaaggccgc gccagttatc tcggtgaacg cagtattggt
     4681 caccaggatc ccggcgcgac ctcggtgatg tttatgatgc aaatgttggc gttagccgca
     4741 aaagagtaag gaattggtga tggtaaacct ggtcatagtt tcacatagca gccgactggg
     4801 agaaggtgtc ggtgaattag cccgtcagat gttaatgagt gataattgta aaatcgccat
     4861 tgccgcggga attgacgatc cacaaaatcc cattggtacc gatgccgtca aagtgatgga
     4921 ggccatcgaa tctgttgccg atgccgacca tgtgctggtc atgatggata tgggtagcgc
     4981 attattgagt gctgaaactg cgctggaatt gctggctccc gagatcgccg caaaagtacg
     5041 tttgtgtgct gcgccgttgg tcgaaggtac actggcagca acggtcagcg cggcctcggg
     5101 ggcggatatc gacaaagtta tctttgacgc catgcatgcg ctggaagcca aacgtgaaca
     5161 actgggttta ccgtcctccg acactgaaat ctctgacaca tgtcctccgt acgatgaaga
     5221 agcccgttct ctggcggtgg tcataaaaaa ccgtaacggc ctgcatgtac gtccggcctc
     5281 ccggctggtt tataccttat cgacatttaa tgccgatatg ttgctggaga aaaacggcaa
     5341 atgcgttaca ccggagagta ttaaccagat tgcgttacta caagttcgct ataacgatac
     5401 gctgcgcctg attgcgaaag gaccagaagc tgaagaggca ctgatcgctt tccgtcagtt
     5461 ggctgaagat aactttggtg aaacggagga agttgctcca cctactctgc gtcccgttcc
     5521 gcctgtttcg ggtaaagcct tttattatca acctgtttta tgtacggtac aggcaaaatc
     5581 aaccctgacc gtggaagaag aacaagaacg attacgccag gccattgact tcacgttatt
     5641 agatctgatg acgttaacag cgaaagcaga aaccagcggg cttgacgata ttgccgcaat
     5701 cttttctggt caccatacac tgttagatga tccggaactg caggcggcgg caagcgaact
     5761 ccttcagcat gaacattgca cggcggaata tgcctggcag caagttctta aagaacttag
     5821 ccagcaatac caacagctgg atgatgaata tctacaggct cgctatatcg atgtggacga
     5881 tcttctgcat cgcaccctgg tccacctgac ccaaacgaaa gaagaactcc cgcagtttaa
     5941 ctcgccaact attctactgg cggagaacat ttatccctcc acagtactgc aactggatcc
     6001 agcggttgta aaaggtatct gccttagcgc cggaactccg ctatcccaca gtgccctaat
     6061 cgcccgtgaa ctgggaattg gctggatttg ccagcagggt gagaaactgt atgcgataca
     6121 accagaagaa acgctaacgc tggacgttaa aacgcaacgt ttcaaccgtc agggttaaac
     6181 caccgttcgg atggcatcat tctgatgtca tccggcaatt tactctgcac tgatgttttt
     6241 taactacctg ttatattttt caccaaatta tgatgatttt tcttcgctaa tcgcgaaccc
     6301 aacgtcgctg gctgacgaat aaacagatgt tattttatca ctctatttcg ccgttattta
     6361 tccctttaat tcccttttct aaaatgtctg tcagttcgca gaatgtgatt tcgatcatgc
     6421 agctagtgcg atcctgaact aaggttttct gatacttgaa taccgtttta ttcggtttcg
     6481 ccaaaggaga atgattgatg aaatcccccg caccttctcg cccgcaaaaa atggcgttaa
     6541 ttccagcctg catctttttg tgtttcgctg cgctatcggt gcaggcagaa gaaacatcgg
     6601 taacaccaca accgcctgat attttattag ggccgctgtt taatgatgtg caaaacgcca
     6661 aactttttcc ggaccaaaaa acctttgccg atgccgtgcc gaacagcgat ccgctgatga
     6721 tccttgctga ttatcggatg cagcaaaacc agagcggatt tgatctacgc catttcgtta
     6781 acgtcaattt caccctgccg aaagagggcg agaaatatgt tccgccagag gggcaatcac
     6841 tgcgcgaaca tattgacgga ctttggccgg tattaacgcg ttctaccgaa aacaccgaaa
     6901 aatgggattc tctattaccg ctgccaaagc cttatgtcgt gccaggcgga cgttttcgcg
     6961 aggtatatta ctgggacagt tacttcacca tgttaggact tgccgaaagc ggtcactggg
     7021 ataaagtcgc ggatatggtg gccaattttg ctcatgaaat agacacttac ggtcatattc
     7081 ccaacggcaa ccgcagttac tatttaagcc gctcgcaacc gcccttcttt gccctgatgg
     7141 tagagttact ggcgcagcat gaaggcgacg ccgcgttgaa acaatacctg ccgcaaatgc
     7201 aaaaagaata tgcttactgg atggacggtg ttgaaaacct gcaagccgga caacaggaaa
     7261 aacgcgttgt aaaacttcag gatggtaccc ttctcaaccg ctactgggac gatcgcgata
     7321 cgccacgacc agagtcatgg gtggaagata ttgccactgc caaaagcaat ccgaatcgac
     7381 ctgccactga aatttaccgc gacctgcgct ctgccgctgc gtctggctgg gatttcagct
     7441 cgcgctggat ggacaatccg cagcagttaa acaccttacg caccaccagc atcgtaccgg
     7501 tcgatctgaa cagcttgatg ttcaaaatgg aaaaaatcct cgcccgcgcc agcaaagcta
     7561 tcggagataa cgcgatggca aaccagtacg aaacgctggc aaatgcccgt caaaaaggga
     7621 tcgaaaaata cctgtggaac gatcaacaaa gctggtatgc cgattacgac ctgaaaagtc
     7681 ataaagtgcg caatcagtta accgcggccg ccctgttccc gctgtacgtc aatgcggcag
     7741 cgaaagatcg cgccagcaaa atggcgacgg cgacgaaaac acatctgctg caaccaggcg
     7801 gcctgaacac cacgtcggtg aaaagtgggc aacaatggga tgcgccaaac ggctgggcgc
     7861 cgttgcagtg ggtcgcgaca gaaggattac aaaactacgg gcaaaaagag gtagcgatgg
     7921 acattagctg gcacttcctg accaatgttc agcacaccta tgaccgggag aaaaagctgg
     7981 tggaaaaata tgatgtcagc accaccggaa cagggggcgg cggtggcgaa tatccattac
     8041 aggatggctt tggctggacc aatggcgtga cgctgaaaat gctggatttg atctgcccga
     8101 aagagcaacc gtgtgacaat gttccggcga cgcgtccact cagtgaatca acaacacagc
     8161 cgctaaaaca aaaagaggcg gaacctacgc cttaacttta ctgtatatcg agcaatgcca
     8221 ttacgtgcat aaaattggca ttgctctttc ttcatcacga aataccactc tttctacggt
     8281 ctatgccttt attttctttt attagagaag attttaattt tattttatca acttgttaat
     8341 catcacacct cta
//
LOCUS       DACADF010000182         8350 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.184, whole genome shotgun sequence.
ACCESSION   DACADF010000182 DACADF010000000
VERSION     DACADF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.184"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            204..1298
                     /gene="ddlA"
                     /locus_tag="ISB30_004214"
     CDS             204..1298
                     /gene="ddlA"
                     /locus_tag="ISB30_004214"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP1991782.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNNNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1322..1534)
                     /locus_tag="ISB30_004215"
     CDS             complement(1322..1534)
                     /locus_tag="ISB30_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HAP1991783.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1794..2102
                     /locus_tag="ISB30_004216"
     CDS             1794..2102
                     /locus_tag="ISB30_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAP1991784.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2161..3255)
                     /gene="yaiW"
                     /locus_tag="ISB30_004217"
     CDS             complement(2161..3255)
                     /gene="yaiW"
                     /locus_tag="ISB30_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAP1991785.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3268..4488)
                     /gene="sbmA"
                     /locus_tag="ISB30_004218"
     CDS             complement(3268..4488)
                     /gene="sbmA"
                     /locus_tag="ISB30_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAP1991786.1"
                     /translation="MFKSFFPKPGMFFLSAFVWALIAVIFWQVGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4840..5997
                     /gene="ampH"
                     /locus_tag="ISB30_004219"
     CDS             4840..5997
                     /gene="ampH"
                     /locus_tag="ISB30_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAP1991787.1"
                     /translation="MKRSLLFSAVLYSASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5998..6621)
                     /gene="iprA"
                     /locus_tag="ISB30_004220"
     CDS             complement(5998..6621)
                     /gene="iprA"
                     /locus_tag="ISB30_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HAP1991788.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6707..>8350)
                     /locus_tag="ISB30_004221"
     CDS             complement(6707..>8350)
                     /locus_tag="ISB30_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1991789.1"
                     /translation="VIDTLAVNNGSQVNVTDSSLVSDTIGLTNGSSLNIGEDGLVATD
                     HLTVDSYSTVNLTESTGWNNYSNLYANTITVTNGGVLDVNVDQFDTEAFRTDKLELTS
                     GNIADHNGNVVSGVFDINSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTING
                     NGDVDNGTELDNSSVDNVVAATGNYKVRIDNATGAGAIADYKDKEIIYVNDKNSNATF
                     SAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRL
                     TNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAA
                     AGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTY
                     VDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNNMKVDGQSYD
                     SMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVG
                     LGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2125 a         2122 c         1997 g         2106 t
ORIGIN      
        1 tggtcattta agccaggaat aaattagctt cccgttcatc atttttgtga tcttgcacac
       61 atctttgaga taacgcactt tcttcaccag tacgttgctc gctttgtcag cagaagattg
      121 caacattctg tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta tgcactttgt
      181 gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt tttggtggta
      241 aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat gccattgata
      301 aaagtcgctt cgatgttgtg ctgctgggca ttgataaaca agggcaatgg cacgtcagcg
      361 atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg cgtccttcgg
      421 cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac gcacaaaacg
      481 gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg ctgggcgaag
      541 atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt tctgatgttc
      601 tggcttcagc ggcctgtatg gataaagatg tcaccaaacg tctgctgcgc gatgccgggc
      661 tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc agttttgccg
      721 aagtggagtc taaactgggg ttaccgctgt ttgtaaaacc ggctaatcag ggctcttctg
      781 ttggtgtcag caaagtaacc agtgaagaac agtacacaat tgccgtcgat ctggcgttcg
      841 agttcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgaaatc gaatgcgcag
      901 ttcttggcaa caataacccg caagcaagca cctgtggcga gatcgtactc accagcgatt
      961 tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta gttccggcag
     1021 ccattgcgcc agaaatcaac gataagatcc gggcgattgc cgttcaggct tatcagacgt
     1081 tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac gaagtggtga
     1141 tcaacgagat caacaccctg cctggcttca ccaacatcag tatgtatccg aagctgtggc
     1201 aagccagcgg tctgggttac accgacctga tcacgcgttt gattgagctg gcgctggagc
     1261 gtcacgctgc ggataacgca ctgaaaacca caatgtaata ttcaaacgcc cggtaatatt
     1321 cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcaatccc gccagccaga
     1381 aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt gcttcaaatc
     1441 ccagtaaccc caccacgcca ttaagaatga atataaggcc aatggcgaac gcatagtagt
     1501 gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg aaaaacaaca
     1561 tcttttcata ttctgtaaca actgtctgtg gtgtagacaa cactttcgca attgttaata
     1621 aatttaactt aaggttgcta ttttgtgact cgcaacgaag attcacgctt cgggaatatt
     1681 cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt cagacttgcc
     1741 ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt tttatggctg
     1801 atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct tcaacgcctg
     1861 gcaatattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggca caattgctga
     1921 acctgctagt gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag gaaacaggtc
     1981 gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt tgggctgatc ccgtttttag
     2041 taggctgcct catttttgca gtagtggcgc tatggctgca ctggcgacat cgccgccagt
     2101 aagatttacg ttgccagatg cggcctaaaa cgccttatct ggcgtgacat tcctgtttga
     2161 tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc aggccgtagt
     2221 cagattgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt cgcggggtag
     2281 tgatttaccg gttttcgctt cggcaagttg ataaactttc ttgtacagcg ccgtctcctc
     2341 aaaagagaag ctgtcccctt tctctaactg acggcgaatt tcgctgtcac tcatccccag
     2401 ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggctctttac tgtcgtaacg
     2461 aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgaccg cgttctgaaa
     2521 cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaaacggt atatcggcgc
     2581 gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc cgccgcggcg
     2641 actgaagact tcctgacgga ctgtaccgtc cattttccac ggataccctt tggtatgctg
     2701 ttcggcaaaa gcaatactga cctgcatcgg cccaccggtg cgcaccggat tgagcgaacc
     2761 aaacagcgtc tgccccattg gcaccatgct gatcagatcg tcaaaaatcg cgctcaattg
     2821 cttttctgta cgcaccgaat ctaaccgttc gctatagctt ttgccgtttg gcgatttgat
     2881 ttttagcgcg gtatgcacca gaaaagcagg aatgtgcatc cgttcagcac gacggtcaat
     2941 ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat aatttgactc
     3001 ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca gcccctgact
     3061 ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc tcgcaggcat
     3121 cttctgccgc acgacgctcg ccacatctat cgctttttcg ccctttttca acggttgcgg
     3181 agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg aagatagagg
     3241 attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt cctgaatttt
     3301 gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca tcaactcaac
     3361 cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac caaaaacgtt
     3421 agtaatctgc gtcatcaggc cgagtgtaat cgtaccggca acaattgacg gaaacagcaa
     3481 gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatat tgaaatacat
     3541 atagtgaaaa tagaggcgga aatagttttt ccgtacggcg ctaaaaagct cgcgtaccgt
     3601 aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agttctttac ggtaggcagc
     3661 ctctacacgc tggtttttaa actccagccc cggcagtttg atccctacca ctgccagtaa
     3721 tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg gaatgtgccc
     3781 gacaatcggc agctccggca catgtgcgga gagcgttacc agcaccggca ggaaggcgat
     3841 caacgtcatg atagcgttga taaagctgac gcccatattc tccagcgttg aagcaaaacg
     3901 catggtgtct tcctgcacac gctgcgcggc cccttcgata tgacgcagtt gttgccagtt
     3961 cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acgtagtgac tgacaaagaa
     4021 gttgttcagc acactgatca ccaccgcgat cagcgcaatc cccagaaaga cgcccacttc
     4081 gcggtaaaat tgttcgatgg tgactttatg cggcgaactt agcgccgttt gaatcagatc
     4141 atagaacggc gcataccagg cgttgacggc aaccccgact tccaccaaaa accaggtgac
     4201 gaagataatc agcgcagtac cgagtattga ccagtattgc caacggtgcg ggctatagat
     4261 aaaccagaac aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa aaatcaggaa
     4321 atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc cggtgatacg
     4381 cgccacccaa tcacccccac cgacttgcca gaagataacg gcaatcaatg cccaaacaaa
     4441 ggccgagaga aaaaacattc ccggctttgg gaaaaaagac ttaaacatat gcactcctgc
     4501 taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccgcta caaagcgtca
     4561 gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca gcctgaccat
     4621 aagaattgtt tcgacgggtt tcacaaacca aagattgttt acataagcgg attaacacgc
     4681 gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac gcgccgttac
     4741 gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga gtagtataaa
     4801 tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag tctgcttttt
     4861 tctgccgtgc tgtattcggc gtcattgacc tccgtccatg cggctcagcc gatcactgaa
     4921 ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta cggcagcggt
     4981 gccacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg cagttatggc
     5041 gaaacgcgac ctggtaataa cgttcgcccg cagctggatt ccgtcatacg tattgcttcc
     5101 ctcaccaagt taatgaccag tgagatgctg gtaaaattgc tcgaccaggg gaccgtgaag
     5161 ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtaccgac ctacaatggg
     5221 acgcccatta cgctggtgaa tctggcaacc cataccagcg ccctgccccg tgaacagccc
     5281 ggtggcgcgg cacatcgtcc ggtatttgtc tggccaacgc gcgagcaacg ctggaaatac
     5341 ctttccactg ccaaactaaa agccgcgccg ggcagccagg ccgcgtactc caacctcgca
     5401 ttcgatctgc tggcagatgc actggcgaat gcgtccggca agccttatac ccagttgttt
     5461 gaagagcaga ttacccgtcc gttggggatg aaagacacca cctacacccc ctcaccggat
     5521 cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa cacgctggcg
     5581 gcaattggca gcggcggcgt ctattccacg ccaggcgata tgatgcgctg gatgcagcag
     5641 tatttgtcat ctgattttta tcaacgcagt aaccaggctg atcgcatgca gacgttgatt
     5701 tatcagcgcg cgcagtttac aaaagtgatt ggcatggatg tccccggcaa agcggatgcg
     5761 cttggtttag gctgggtata tatggccccg aaagagggtc gtccggggat tattcagaag
     5821 acaggtggtg gtggcggttt cattacctat atggcgatga tcccgcagaa gaatattggt
     5881 gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta aaaatatgag cgatggcatc
     5941 aatgacctgg tgaccgagct aagcgggaat aaaccgttgg ttatccccgc atcctgatta
     6001 atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt cgatatagcc
     6061 gcctttacgc aaagcagcga gaacttccgc aacgacagaa cgcgatatac gtgtacgttg
     6121 atggatataa ttcatcacgc caatacgtga tcgcaattct tcattccagt caatcattga
     6181 taataacgtc gcgcggattt gttcgtagga attatgccca atgagctgaa cgtcgcgtaa
     6241 ttccagaatt ctattttgcc aggctaacca gtaaaaagcg tctcgccaga gttgattttg
     6301 ttcaataaga gtaatggttt gtttggctgg caaatgatat cccgtacagt ttccttctga
     6361 tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca taatataagg
     6421 agcctgtgta ataccgataa gtacgttttc ttctctacgc agagagataa caccttctaa
     6481 aataacgaaa gtatcttcgc tattgacatc actggaaaat ataacttgct tttcattatt
     6541 aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa attcctgaag
     6601 aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca aatccttgac
     6661 gggcattatt tagattaaat tacctatttc ttcggagtga agaatattac caggtatatt
     6721 taacacccac attcgcggac cagtcttgat ctacgtcacc accaccgagg tagttagcat
     6781 cggtataggc gctgaagttc ttggtgaagc tgaactgagt acccagacca acacgtaccg
     6841 cagacccttc agtaccgtta tcgatggaat caccgttcac atcgttatcg ttgttagagt
     6901 cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc ctggtcttcg ctgtaggtga
     6961 aggtataacc tgcatctaca cccagttcat aacgcatgct gtcgtaagac tgaccatcaa
     7021 ctttcatgtt gttgctcagc tggtaatcat caccagactg gaacagacca gaaacgctgc
     7081 cgtaaggagt tacgtaacct gcatcaccca gtttgaagtc gtaaccggct ttcaaaccaa
     7141 agccccaagc gtcggagttg gtgctgccgt caacgtaagt accgttgctc atggttgcag
     7201 acaggtcgtt gttgaagtga gagtagctca agctaccgtc aacaaagacg ttgttcgcga
     7261 agtgagcaga agagtagatg taggcagtct ggctgtcttg atccacctga ccagaacggt
     7321 cattcatatc acctttagcg aagcctgcag ccgcaccgac gatccactta gcgttgttac
     7381 cgtcaatttt ggtatcaaca ccgaccatga tgccgttaac atcctgatca tagttgatgg
     7441 tgccgttgtc gccgttgaag ttaccaccga agtagcttac ccatgcgccg ccgttatcag
     7501 ccagaccatg acgagagttg gtcagacgag taccaacggt gtcttgttcc aggttccaga
     7561 tattggtgtt cgcagacggg atgctcagcg ccatgttagc gtagtcggtc agctccatct
     7621 gttgcagaac aacggtgtta ccacgctgct cagcctgata ggtgtatgca cccaggtcag
     7681 ctttgttagc agcagagaaa gtcgcgttgc tgtttttgtc gttgacgtag ataatttctt
     7741 tatctttgta atcagcgatt gcgccagcgc cagttgcgtt gtcgatacga actttgtagt
     7801 taccggttgc agcaacaacg ttgtctacag aactgttatc cagttcagta ccgttgtcta
     7861 cgtcgccgtt gccattgata gtcaggtgac catcagagtt catagcaaca ataccgtaac
     7921 cgtagttaga cttggaagta tcccacgtac ggtcgttcac caaatccgcg ttcagaacgt
     7981 aatcgctgct gttgatgtcg aacacaccag aaactacgtt gccgttatgg tcagcgatgt
     8041 tgccgctagt cagttccagt ttgtcagtac ggaaagcttc agtatcaaac tgatcaacgt
     8101 tcacatccag tacaccaccg ttagttacgg tgatggtatt ggcatacagg ttagaatagt
     8161 tattccagcc agtagattcg gttaaattaa cggtactgta gctatcaaca gtcaggtggt
     8221 ctgtagcaac caaaccatct tcaccaatat tcagtgagga gccgttggtt aaaccaatgg
     8281 tatctgagac cagagaggaa tcagtaacat ttacctgaga gccattattt acagctaatg
     8341 tatcaattac
//
LOCUS       DACADF010000183         8215 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.185, whole genome shotgun sequence.
ACCESSION   DACADF010000183 DACADF010000000
VERSION     DACADF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.185"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            14..868
                     /locus_tag="ISB30_004222"
     CDS             14..868
                     /locus_tag="ISB30_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1991790.1"
                     /translation="MEQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDERLKL
                     EIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSN
                     HNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGER
                     MTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCY
                     DNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFR
                     EKYHQMAA"
     gene            1023..3020
                     /locus_tag="ISB30_004223"
     CDS             1023..3020
                     /locus_tag="ISB30_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HAP1991791.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATGLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            3083..4360
                     /locus_tag="ISB30_004224"
     CDS             3083..4360
                     /locus_tag="ISB30_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HAP1991792.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATHADHIDSDRYLIKSTYYNLFSGEYSNKKT"
     gene            4608..5264
                     /locus_tag="ISB30_004225"
     CDS             4608..5264
                     /locus_tag="ISB30_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP1991793.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVKNANVPKGSFYYYFKSKEDYAQTVLNAYDSFFEHKLKKHLHESSCTPMVRLENFI
                     NDACEGIKKYNFTRGCLVGNMMQESPGLPQSFIKVLQNILESWQALVAACLSDALSSG
                     EISSNMNNTQLAAIFWSGWEGAVMRSKLYCSTEPVYDFWSYFKTSVRYQSSQEATTGQ
                     "
     gene            complement(5445..5552)
                     /locus_tag="ISB30_004226"
     CDS             complement(5445..5552)
                     /locus_tag="ISB30_004226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAP1991794.1"
                     /translation="MGNMAPFILRGVTLKGIDSVMFAKEKRAAIWQQAV"
     gene            6678..6776
                     /locus_tag="ISB30_004227"
     CDS             6678..6776
                     /locus_tag="ISB30_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HAP1991795.1"
                     /translation="MISQIDKLEYVVKVQRNQSNPTMFNKIVVFFQ"
     gene            complement(6778..7560)
                     /locus_tag="ISB30_004228"
     CDS             complement(6778..7560)
                     /locus_tag="ISB30_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP1991796.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            7866..>8215
                     /locus_tag="ISB30_004229"
     CDS             7866..>8215
                     /locus_tag="ISB30_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP1991797.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLS"
BASE COUNT         2153 a         1835 c         2013 g         2214 t
ORIGIN      
        1 aaaatacgcg ttaatcgaac aatggcgaca acaatttccc attgaagcga tgtgtcaggt
       61 atttggtgta tccaggagcg gttattacaa ctgggtacag catgaaccct cagacagaaa
      121 acaaagtgat gagcggctaa aactggagat taaggtggca catatccgca ctcgcgaaac
      181 atatggaacc cggcggctcc agacggagct ggcagagaat ggcatcatcg ttggtcgtga
      241 ccgactggca cgtcttcgta aggagctaag gctacgctgt aagcagaaac gcaagttcag
      301 agcgactacg aactcgaacc acaatctgcc agttgcgcca aatctgctga accagacgtt
      361 cgctcctaca gcaccaaatc aggtctgggt ggcggacctg acgtatgttg ccacacagga
      421 gggatggttg tacctcgctg gcatcaaaga tgtttatacg tgcgaaattg tcggctacgc
      481 catgggagag cgcatgacaa aagagctgac aggtaaagcc ctgtttatgg cgctcaggag
      541 ccagcgccca cctgccgggc taatccacca ctctgatcga ggttcacagt actgcgcata
      601 cgattaccgg gtcatacagg agcagtttgg tctgaaaaca tcaatgtcgc gtaaaggtaa
      661 ctgttacgac aacgctccga tggaaagctt ctggggaacg ctgaaaaatg agagcctgag
      721 ccactatcgt tttaataacc gggatgaagc catctcagta atacgggaat acattgagat
      781 tttctacaat cgtcagcgtc gtcactctcg tctggggaat atctccccgg cagccttcag
      841 ggaaaaatat catcagatgg ctgcttaaaa aaaagaacaa atggtagtgt ccgctattgc
      901 cagtacacct caacattcca ccatgcattc cgattaacgc cgcatagcca gttgaacttt
      961 gctactttgt gagaggtagt accttctatc cagtgcgaat ttaattaatg gaataaatga
     1021 ttatgagtga aaatgataca atcccaaaga agtctacaag tcagattaac aaagcggtat
     1081 tctttacatc tgctttgcta attttccttc ttgtcgcctt tgccgccgta ttcccggatg
     1141 tcgccgacaa aaattttaaa ctacttcagc aacaaatctt cacgaatgcc agctggttct
     1201 acatccttgc tgtggccctg attttactga gtgtcacgtt ccttggactc tcacgctacg
     1261 gtgatatcaa gctgggcccg gaccatgcgc agcctgattt cagctaccac tcctggtttg
     1321 cgatgctttt ttcggcaggg atggggatcg gcctgatgtt ctttggcgtt gccgaacctg
     1381 taatgcatta tctttcgcca cccgttggca ctccagaaac cgttgcggca gctaaggaag
     1441 caatgcgtct gacctttttc cactggggac tgcacgcatg ggcaatttat gccattgtgg
     1501 cgctgattct ggcgttcttc agttaccgtc acggtctgcc tttaactctg cgctccgcac
     1561 tctatcccat tattggcgat cgcatatacg gacctgtagg acatgcggtt gatattttcg
     1621 ctgttatagg cacggtcttt ggcgttgcga catcactggg ttacggtgtt ttgcaggtga
     1681 atgccggttt gaaccatctt ttcggggtgc ccatcaatga aacggtgcag gtaattctga
     1741 tcgtggtcat cacggggtta gcgacgattt cagtggtgtc cggtctggat aagggaatac
     1801 gtatcctgtc tgaactcaat ctgggtctgg ctttgttgct cctggcgctg gtcctgtgtc
     1861 tgggaccaac cgtgcttctg ctgaagtcat ttgtggaaaa tacgggcggt tatctttcgg
     1921 aactggtgag taaaacgttc aacctttacg cgtatgagcc caagtcgagc aactggctgg
     1981 ggggctggac attactgtac tggggatggt ggctttcatg gtcgccgttt gtggggatgt
     2041 tcatcgcacg ggtctcccgc gggcgaacca ttcgcgagtt tgtcaccggc gtgctgtttg
     2101 ttcccgcggg ttttacgcta atgtggatga cggtgtttgg taacagcgcg atctatctca
     2161 ttatgaacca gggggccaca ggcctcgcca atactgttca gcaggatgtg gcgctggccc
     2221 tgtttaattt cctggagcat ttcccgttct cttctgtgct gtcattcatt gcaatggcga
     2281 tggtcatcgt cttctttgta acgtctgctg attcgggggc aatggttgtg gatactctgg
     2341 catcaggtgg agtggcaaac acacccgtct ggcagcgaat attctgggcc tcgctcatgg
     2401 gcattgttgc aattgcgctt ctccttgccg gagggctaag tgcgctgcaa acggtgacaa
     2461 tagcgagtgc attgcccttc tcagtgatct tactaatatc tatatacgga cttttaaaag
     2521 ctttgcgccg ggatttgacc aagcgtgaaa gcctgagcat ggcgacaatt gctcctacgg
     2581 ctgcacgtaa cccaattcct tggcagagaa ggttacgcaa tatcgcgtat ctgccgaagc
     2641 gatctcttgt gaaacgtttt atggacgacg ttatccagcc cgccatgacg ctggttcagg
     2701 aggaactgaa caagcagggg acgataagcc acattagtga tgcagtcgac gatcgtattc
     2761 gtcttgaagt cgatttgggc aacgagctga attttatata tgaagtgagg cttcgcgggt
     2821 atatctcacc gaccttcgcg ctcgccgcaa tggataatga tgagcagcag actgaacaac
     2881 atcgatatta tcgcgctgag gtttatctca aagaaggcgg tcaaaattat gatgtgatgg
     2941 gctggaacca ggaacagctg attaatgaca tactggacca gtacgaaaaa cacctgcact
     3001 tcctgcacct ggttcgttaa tagcaacatg ccgtcctggg ggcggcaatt attatcccgg
     3061 ccgcaatatg agggaatgca gaatgatttc acgctggaaa tggatgctga agcagacaat
     3121 taaaaaacta tggttcaggg caacgttatt cgcaattgtc gcgataataa cggccctttt
     3181 atcaattctt tttaaatcaa tgatacctga gtcggtttcc gtgaaggttg gtgcggaagc
     3241 agtcgataac attctgaaca tactggcatc gagtatgctg gcagtgacca cattttcgct
     3301 gagtatcatg gtcacagcct acggttcagc cactactaat gtgactccca gagctacgcg
     3361 tttagttgtt gaagacgtca ccacacaaaa tgtactggcc accttcatcg gttcttttct
     3421 cttcagtctg gtagggatta ttgccctcaa tatgggagct tatggagaaa gggggagagt
     3481 cattttattc attgtcacac tggttgtcat tgccttaatc ctcatcacat tgcttcgctg
     3541 gatacagcat ttgacctctc tggggagggt tggtgagaca acggcacaag tagaacaggc
     3601 ggccatcgaa acatttattg cgagagcaag aaatccctgt ctcggcggat atccatggct
     3661 tgagaacaat gaacagccga aaggaacagt tgcagtttat ccgaagaaga ttggctatgt
     3721 tgaatatatt gatatggtga aacttagcaa gctgctgacc aatgatcccc gtcatgtata
     3781 cctcgtggcg cagccaggca gtttcataca tccgtccatg ccagttttgt acctgagtca
     3841 gggccaggag tcgtcaatca acaccgattt acttgagacg attattgtct cggatgcacg
     3901 ttcatttgct caggatcctc gattttgtct tagcgtcatg gccgaaatag cctgccgggc
     3961 cctttcccct gcagtgaacg atcctggaac cgccattgat gtcattggca gaggtgttcg
     4021 tatactttcc acttacgcgc agaataaatc tgatgaaata gaagtgaaat atccttcagt
     4081 acatgttgca ccacttcaga ataacgatct actggaagac tttttctcac ctgtcgcgcg
     4141 cgatggtgcc agtatgaggg agattcagat aagagtcctt aaaggactgt cgatgctgag
     4201 taaaggttgg cctgagatat ttgctgaagc cgcacatacc ctggcatttg aaacattaga
     4261 gcatgcaact catgctgacc atatagattc tgatagatat ctaataaaat caacttatta
     4321 taatttattt agtggcgaat attctaataa aaaaacatag ctgcagagca agcaattcgg
     4381 ttgttggcaa gtcaagggca aagcattcgc gtgagaatgg gcgtatgctt tgtgccaaaa
     4441 gcaaacgttg ctaattctgc actgttcagc gatggcgcag tagcgacagg cactgccact
     4501 gtccagtcgg caaaagcgaa cactttgcca ctgcggggat aaactgattg aagctctcgc
     4561 gcaaaatcac ttattctttt gcgttaataa aatgtaggag atgggtcatg acaggcgaaa
     4621 atcgtggccg gggaaggccg cgaaagacag aaagtaccta tgcagatact cgtaatgatc
     4681 tgatccgcag tggccttgaa ctgctgacgc agaatggttt tcttgcgacg ggagtcgatg
     4741 ccattgttaa aaatgccaat gtccctaaag gctcatttta ttactacttt aagagtaaag
     4801 aggattatgc tcagaccgtt ctgaacgcct atgacagttt cttcgagcat aaactcaaaa
     4861 aacatctaca tgaatcatcc tgcacaccaa tggtacgtct ggaaaatttc atcaatgatg
     4921 cctgtgaggg gattaaaaaa tacaatttta cccgcggctg cctggttgga aatatgatgc
     4981 aggagagtcc ggggcttccc caatccttca ttaaagtact acagaatatt cttgagagct
     5041 ggcaggcact tgttgcggcc tgcctgtccg acgcgttatc ttcaggcgaa ataagttcaa
     5101 atatgaataa tacacaactg gcggctattt tctggagtgg ctgggaaggt gcggtaatgc
     5161 gttccaaact atattgttct actgagccag tgtacgactt ctggagctat ttcaaaacct
     5221 cggtacgcta tcagtcttct caggaagcaa caactggcca gtaaaagggg ccgcagcccc
     5281 taaaacttat tatactaagc ctgagctcgg catccatcta attagctata ttgaatgact
     5341 gcacgaccgc gaattccgcc actcagaagt ttttcagcca cagtgatggc gtcactgagc
     5401 ggataaacgg tagtgagact atccattctg gactctggca aatactatac cgcctgttgc
     5461 cagattgctg ctcttttctc tttcgcgaac atgacgctat caatcccttt cagcgtgaca
     5521 ccacgcagaa taaacggagc catattgccc atcaaatcca tcccctgagc catgccgcag
     5581 gctgcaacaa tactgccata tcggaccccg gcgcaggcat ttctccagta acagggggtt
     5641 gtattgtatt gtattttatt gtgccggttc aggtgtgcgt ttcccggcgc gtctgcaccg
     5701 gcttaaccaa attcaacagg gataaaatag tgcaaataac cgaagtatta cttgcagtaa
     5761 tcgggggaag catccggggt tttattcaac aggctgtaga tcactgactg cgtctgttgg
     5821 ccgtccactt cacgcgtcat tcgaccagag taaatattga tgattactag attcgtgtat
     5881 tttatcaggc attttacttg cgaataaatc cccttgctat ccagcgaaac cacacaggta
     5941 gctattacca tatgtggtaa tacactagtt acaggaaaaa tatgcaagtt ccgcaatacg
     6001 ttgtgtgttg ctccttagcc aggatctttt cttccatgcg agatatgaca acaattttga
     6061 ggaaggatgt tcgtaactgg tagagttact acaacaagca tgggaggctc tgactctgac
     6121 agacattgta ctgtggaatg ttgttacagg ttggggcggc aaccgtgcca ggaaaccatg
     6181 agccgttcac ggcattaaag gccacacttc agtcgtttac attccctatg gtggcaggca
     6241 tcttccatta aggcgtcaga gcgttaccaa cgttatgttg tcatggctca tctttcatag
     6301 tcgatgctta tctggtggct gaagctagcc gcagataaag agtgtgaagt tgataccatg
     6361 gagtggagtg acgtgcaggc aggagattcg ctctatgcag ggttgtgaga tgaattcaaa
     6421 ccggctggca gcgccagtga tattcgaaga ttcgtccggt tgttacccag tatgcattaa
     6481 aaacccatac attatggaca atataacggc ttctacaaca aggaagtgcc tgtttaccat
     6541 ccgggtgtaa tgactcacct ttgtgggctt ttgcccgtcg cgctcttaac gttgaagaca
     6601 ttctctgcct gccgattcag gactgcgaca aaagccatat ctggctactg gtcaaggatg
     6661 accagcgtct ggagtaggtg ataagccaaa tagataagct ggaatatgtg gtgaaagtgc
     6721 aacgtaatca gtccaacccg acgatgttta acaagattgt ggtgtttttt cagtaagtca
     6781 ttcgtcttct gaatcaggtg taatgatctt tacagaacca ttctggcagt gcgtaacata
     6841 atctgccggt aacggacgat cgctaatcag aacgtcaaat gcagagatcg gtgcgaggct
     6901 ggcagaggcc acttcatcga acaacgcgtg gcgggcgagc aaaattttcc gtagtccacg
     6961 gttcatcgct ttacgcttag tggcaagatc ttcagggtta aaccagctga cgccaaagtg
     7021 attatgcaca ccgctggcgg aaataaatat ttttcgcgga ttgagagagt ctaatggcga
     7081 agagttactg gcatcgtaaa aagcatcact tctggcacga tacgtaccac cacaaagtat
     7141 tgctgttaca ttaggctttt cattcaacgc aacaaagacg cgatgtgagt aacagatgcc
     7201 ggtgaaggtg attgcatccg gaatcatgct tataacgagt ggtatctcct ggccattatc
     7261 aaagaagatc agatcatttt cattaaccat tccggcagcc agaattgcaa taggtaagtc
     7321 atcacgatga ttttttggaa cgtcatggat tactggcatg gatggcgcgg gtttattcac
     7381 cattacaata tagccaccca gtagggtcag tggcagaggt tcatcttcct gatggagatc
     7441 gcgacgaata gtcattacag aaacttccag cattcgcgct gcgtctttca aatggattct
     7501 gtcggttttc ttaagcagtt ccatcaaacg tcggatacgc tctttttgct tcgtttccat
     7561 caactctctc tccgtaagaa tgttataaaa ataacatttt ggcgttagta aacattgttt
     7621 tcattaccat actatacgac cgcccctttg aaatgaaaag ggtataggct gctgagtttt
     7681 gggcactaat cttgttaggt tgcttatgat tagatagtgt tggcctgagt gatgagactg
     7741 gaagtctatt aaataaacat cgggttgatg tgctaatcgc gatctcagtc acaaatgatg
     7801 caaaagtaac atgctaatgt tatttaaata ccattgttat gcgaaagttt ataagaggta
     7861 gtgaaatgga cattgcagtt attggttcca atatggtgga tctcattact tacactaatc
     7921 agatgcctaa agaaggagaa actctggaag cacctgcctt caaaatcgga tgtggtggga
     7981 aaggtgctaa tcaagcggtg gcggcggcta aattgaattc aaaagtcctg atgctgacta
     8041 aagtaggtga tgatattttt gcggataata ctattcgtaa tcttgaatca tggggaatta
     8101 ataccactta tgttgagaag gtcccctgta ccagcagtgg tgttgcaccg atctttgtta
     8161 atgccaactc gagcaatagt atattaataa ttaagggagc gaataaattt ctttc
//
LOCUS       DACADF010000184         8195 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.186, whole genome shotgun sequence.
ACCESSION   DACADF010000184 DACADF010000000
VERSION     DACADF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.186"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..257
                     /locus_tag="ISB30_004230"
     CDS             <1..257
                     /locus_tag="ISB30_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991798.1"
                     /translation="IAKSVLENKDSVVNGRDIDTLPAQIKPIAYQLRNRFKNEGGIIF
                     DFPAAVAVSFSRGDILHSFRFALSGDETLWQLEDAGSSPA"
     gene            complement(264..455)
                     /locus_tag="ISB30_004231"
     CDS             complement(264..455)
                     /locus_tag="ISB30_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991799.1"
                     /translation="MSEDKKVVNLLSELSAALDNINPPRVKTTGDKDALNTEIHILLA
                     LVTDKIQRKYPPVTQLVDF"
     gene            593..943
                     /locus_tag="ISB30_004232"
     CDS             593..943
                     /locus_tag="ISB30_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HAP1991800.1"
                     /translation="MKLQRNSSQQRFRNGAERHANRFATSASRSNSRYSLSETHATPD
                     GYPVKQIGEHTWLIEKAGIVVHRCQRNPFTGNRIFALSNGDNQFGQDFTLYEALRTVD
                     RLLHGQSFIKQADL"
     gene            954..1232
                     /locus_tag="ISB30_004233"
     CDS             954..1232
                     /locus_tag="ISB30_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991801.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVHEIISEAEKARKTNGDEPYIEIICMDSEAEIQKAD"
     gene            1244..1486
                     /locus_tag="ISB30_004234"
     CDS             1244..1486
                     /locus_tag="ISB30_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 domain-containing protein"
                     /protein_id="HAP1991802.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            1483..1596
                     /locus_tag="ISB30_004235"
     CDS             1483..1596
                     /locus_tag="ISB30_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991803.1"
                     /translation="MSIFMSWIVLIISVVCAIGIVRIIHSVKKIERFFTGE"
     gene            1690..2100
                     /locus_tag="ISB30_004236"
     CDS             1690..2100
                     /locus_tag="ISB30_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991804.1"
                     /translation="MHAKEEGIIRALKEISKTENEVAKKAIANNHMDVATHTLIVARV
                     TAEAAEIIAKQDAELAVLRTQPVTGLDLSNTGRLIYTIGSELQRYTIIAGLQDKYLIT
                     PHPIRESEILTNLRLIERSQVAFIDDAQCTVFNA"
     gene            2124..2327
                     /locus_tag="ISB30_004237"
     CDS             2124..2327
                     /locus_tag="ISB30_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991805.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            2324..2590
                     /locus_tag="ISB30_004238"
     CDS             2324..2590
                     /locus_tag="ISB30_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP1991806.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRQLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEART"
     gene            2587..2886
                     /locus_tag="ISB30_004239"
     CDS             2587..2886
                     /locus_tag="ISB30_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991807.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAALALLDEL
                     EHARTTAPAIRLTLHHEIADFCATLEVPGEPETPEAIQQELLQRIDKVFDFFLNQ"
     gene            2898..3515
                     /locus_tag="ISB30_004240"
     CDS             2898..3515
                     /locus_tag="ISB30_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAP1991808.1"
                     /translation="MHTQKNRLPCRNQSGYISAAPHKTGAGFRSLLTTQAHNRASGFF
                     VRTVLPRFFRVRIMAGRTGPTSVGPGSCVAGTANLVRLATHSFAALDGEFSQLTTQGA
                     TPWQTANSTALSRSVVTSRLKSTADFPAHHASRKSCTSICCMSAATHYQTFIPPLFSA
                     IWRMICTSFNSSSSSKTNSINSCSGPFLHLAAGGLRTSVTRGLPQ"
     gene            3512..3877
                     /locus_tag="ISB30_004241"
     CDS             3512..3877
                     /locus_tag="ISB30_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991809.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            3884..6706
                     /locus_tag="ISB30_004242"
     CDS             3884..6706
                     /locus_tag="ISB30_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HAP1991810.1"
                     /translation="MTAEYLRDWQQPRHAVGREGTGIPAPESALSSWLDAYRAENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDSTDGRWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGNDITPRFKVEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVEVNEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLAQLGASD
                     PEAAARLASTLQQGQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRSDI"
     gene            6783..7742
                     /locus_tag="ISB30_004243"
     CDS             6783..7742
                     /locus_tag="ISB30_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP1991811.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGMQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVM
                     VVGGGAEIVAPAIREVCGVNATFIADGVPQFALVNGLYAMDKE"
     gene            7747..8061
                     /locus_tag="ISB30_004244"
     CDS             7747..8061
                     /locus_tag="ISB30_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HAP1991812.1"
                     /translation="MTTPTRRISFYLKPAAVKSEQEACNYLDSLPASERSRAQRAAFL
                     AGLALIKRNPAFAYWMAEWPEDNLPFNTVSIKETYQDNQPSGVECNFYKIKKNIQTLF
                     PE"
BASE COUNT         2240 a         2200 c         2054 g         1701 t
ORIGIN      
        1 taatcgctaa atcagtactt gaaaacaaag attctgttgt caatggaaga gatatagata
       61 ctcttcctgc acaaatcaaa ccgatagcct atcaattgcg taatcgtttt aaaaatgaag
      121 gtgggattat atttgacttt cccgcagctg tagccgtgag cttctctcgg ggggatatac
      181 ttcactcatt tcgttttgct ctatcaggtg acgagacatt atggcagcta gaagatgccg
      241 ggagcagccc ggcataaatc aaattaaaaa tcaaccagtt gtgtgacagg tgggtattta
      301 cgctgaattt tgtcagttac taacgccagt aatatatgaa tctctgtgtt taatgcgtct
      361 ttatcacccg tcgtttttac cctgggaggg ttaatgttat ccagagcagc actaagttca
      421 ctcaacaaat taacaacttt tttatcttct gacatgaaaa cctcttaaca atttaattaa
      481 acataagcca ggacctgaac cggccagtcc aagtcctaga cacacaaaaa aaataccaca
      541 aatcaataca aggtgacacg agtacacctt accgaaggta cagagtttta atatgaaact
      601 acagcgcaat tcttcacagc agcgcttccg caacggtgcg gaacgccatg ctaaccgttt
      661 cgctaccagt gcatcacgta gcaactctcg ctacagcctg agcgaaacac acgcaacgcc
      721 ggatggctac ccagtaaaac aaattggtga acacacttgg ctgattgaaa aagcaggaat
      781 cgtggttcac agatgccagc gcaacccatt taccggaaac cgcatttttg cactgagcaa
      841 cggcgacaat cagttcgggc aggatttcac attatacgaa gcacttcgca cggttgatcg
      901 tctgcttcac gggcaaagtt ttattaaaca ggctgattta taacaggtgc tttatgacca
      961 aagagcatgc acaaggtgta tttatccgtt ttattgattt tcgcggtgaa ctgttattac
     1021 gcgcatcagc cattgacgga gttgttccat ccgaaaaaaa tgcagctact tacgtttatc
     1081 tgaacggcac gcgcctgacc gtagaacttc cgtaccagac tgtacacgaa atcattagcg
     1141 aagcggaaaa agcacgtaag actaatggcg atgaacccta tatcgaaatt atttgtatgg
     1201 attcagaagc agaaattcag aaagcagatt aaagggcgtt gcgatgggca aagaatataa
     1261 aactctcatt aacaaagcac ttgagcgttt ttattttcgc ttaagtgcat caggcgctca
     1321 tgctgaacgt gcagcccgtg actcattgac cagggcaatc cggagtctgt atgacgtggc
     1381 tttttacgct gatgatctgg atgcacttaa cgaactttcc gagctgatct gtgccgcaga
     1441 atgcggggaa catattgaac cgtataagct ggggaatatc gcatgagtat atttatgtca
     1501 tggattgttc tgattatttc agtggtctgc gcaattggca ttgtgcgaat tattcattca
     1561 gtaaaaaaga ttgaacgttt tttcactggt gaataacgat acaaataaaa catcaaatta
     1621 aataagaaaa cgtgaaaacc atccgtatta acggaggtat tcgcacacgc aaataacgga
     1681 gatacaaaaa tgcacgcaaa agaagaaggt atcatcagag cactgaaaga aatttcaaag
     1741 acagaaaacg aagtagcgaa aaaagccata gcaaataatc atatggacgt cgcaacccac
     1801 acactgatag tcgcaagagt cacggcagaa gcagccgaaa ttatcgcaaa acaggatgct
     1861 gaactggcgg ttctcagaac acaaccagtc accggactgg atttgtctaa caccggacgc
     1921 cttatttaca caattggctc ggagctacag cgatacacca ttatcgccgg gttacaggat
     1981 aaatacctga tcactcctca ccccataagg gaatcagaaa ttctgacaaa tctccgcctg
     2041 atagagcgct ctcaagtcgc attcattgat gacgcacagt gcaccgtatt taacgcatag
     2101 agttactgga caaagggggc gcaatggcaa ttaagcattt tcccgtcgtt cgctttacct
     2161 ccagagggcg cgaatacgag gtcgacgaac gcctgattac cactatcgac aaacatcgtt
     2221 cggaaaagga tgcacaccac atctacctca ctgacggcac ttacttctgc gccactaatg
     2281 tggcgcgggt aaaccttatc cgacaggtac aggagccacg cagatgacca ttctggacta
     2341 catcgctact catccggggt gtagcggcgg agagatcgcc gcagcactga atactccaac
     2401 cacagccatt aatgctgagt tacgccaact ttggcgcggc ggcttagtca tcagaacaaa
     2461 ccgcagcaca ggtggtcgcg ctcgcaaaac tggaggccag gcttcttacc acgtaaaccc
     2521 gatgccgttc gggtgtagca atccacttac tcacatgttt aaccagctac tgaaggaagc
     2581 cagaacatga gcaccatcaa ccaccagaag ctacgcgaac tggcatttgc cctgcaacga
     2641 atggcaacac ctcaaaaatt actggcattt cgcgcaatgc tctcgccgtc tgctgcgctg
     2701 gcattgctgg atgagctgga gcacgccaga accacggctc ctgccattcg cctgacgctc
     2761 catcatgaaa tcgctgattt ctgcgcgaca ttggaggtgc caggcgaacc ggaaacgccg
     2821 gaagcaatac agcaagagct gctgcaacgt attgacaagg tttttgattt ttttctgaac
     2881 cagtaagaaa ccagagcatg cacacacaaa aaaaccgctt gccatgccgc aatcagtcag
     2941 gttacatttc cgctgcacct cataaaacgg gtgccgggtt tcgcagcctg ctgactacac
     3001 aagcgcacaa ccgcgccagc ggtttttttg tgcgtactgt attgccacgt ttttttcgcg
     3061 tcagaattat ggcggggcgt acggggccga cttcggtcgg gccgggttct tgtgtagccg
     3121 gtactgcgaa cctcgtacgt ctcgccaccc acagtttcgc agctctggat ggtgagtttt
     3181 cacaacttac tacacaaggg gccacaccat ggcaaaccgc aaacagcacc gcgctatcgc
     3241 ggagcgtcgt cacatccaga ctgaaatcaa ccgcagactt tcccgcgcat cacgcgtcgc
     3301 gcaaatcatg cacatcaata tgctgcatga gcgcagccac gcactatcaa acatttattc
     3361 cgcctctgtt ttcagctatc tggcggatga tctgcacgag cttcaacagc tcatccagca
     3421 gcaaaacaaa ctccattaat tcctgttccg ggcctttcct gcaccttgcg gcgggaggcc
     3481 ttcgcacatc tgtaacaaga ggattgccgc aatgattctc gccaacgact ttcttgaata
     3541 cctgctcaat acagaacgtg atcttgccgc tcgcgtgcgt gatcgttatg acatgtacct
     3601 gaaatccctg cctgtaccgc agctcgctga cggaaagatt gttattgatg gtcgctacat
     3661 gattgacagc cacgagggaa attacaggct ttaccgcatt gaaggtggca ccccgtccgt
     3721 tattggcatt taccagcgcc catcctctgc aatcgtcgat gtgattgccg acagcatccg
     3781 catcacacat cgccatgccg acacagaaga caccgtgctg gaaattcagc ggctggctac
     3841 agtctgccgc gacaccctga atggcatgac gaagtaaatc actatgacgg cagagtacct
     3901 cagggactgg caacaaccgc gccacgcagt ggggcgtgaa ggaacgggga tccccgctcc
     3961 tgaatccgcg ctttcctcct ggctggatgc ctaccgggca gagaacgagc gccgccagga
     4021 aatggctgat gcggcgttct cagccacgcc gctgggcaac ctgattaata aaagcctgga
     4081 cgcacaggaa aaacaggaca aaaccatcac actggcagga gacgccagaa aacaggcacg
     4141 tggcgcggtg gatgaagcca tggcctcgct gcgcctgctg ccgtcctatc tgcgcgatcc
     4201 gcttattcgc cacctctcct tcctgcgcaa aaaacaggaa gccgatcgcc ggaaaggcaa
     4261 aaagagctgg caggcggaac gctatgcacg cggaaccctg cgcaaaatat tcgaacgtct
     4321 ggatagcact gacggacgct ggctgacacc gggttatcgc tcccttgccg gacgcgaacg
     4381 cctggacgat ttgctttacc tgccgcagct caacaaacac cagatacaga cgctggccac
     4441 catgacggcg gcgatgttca gcagcacctt cgaaaaactc tgcgatggct ttggcgcgac
     4501 cgatggcgag ctgaccatgg atgtaaccct gaaggcgtat cagatgctgg cccgcatggc
     4561 gttacacctg cacgccatgc ctccacatta tgacgcactg acaacagaca aagaccggag
     4621 gaacgaaccg gacacggagc tgctgccggg cgcaatcctt cgcctgacct gtgcggaatg
     4681 gtggaaacgc aaactgtggc tgttacgttg cgagtggaga gaagaacaac tccgcgccgc
     4741 ctgtctggtt tccagaaaaa catcacccta tctgagccag gacgcgttaa gcgagtttcg
     4801 cgcacagcgc gagaaaacac gcgatttcct gaaaagtttc atgctggaaa atgaagacgg
     4861 gttcacgatt gatctcgaga cagtgtatta cgcgggagta agtaacccgg ttcaccgtaa
     4921 ggcagaaatg atggccacca tgaagggact ggaacttctg gccgaagccc gtggcgacaa
     4981 agcggtgttt ctgactgtca cctgcccgtc aaaataccac gccacaacgg agaacggtca
     5041 tccgaatccc aaatggaacg gggccacaat gcgcgactcc agcgattacc tggttaacac
     5101 gttttttgcg gcggtccgca aaaaactgaa ccgcgacggc ctgcgctggt atggcatccg
     5161 cacggtggag cctcatcatg acggcaccgt gcactggcat atgatggtct ttgcacatcc
     5221 ggaagaaatc gacaccattg tgtcccatac ccgcgatatt gccattcagg aagaccgcca
     5281 cgagctgggc aatgatatta ctccgcgctt taaggtggag tatgtcgacg gctcaaaagg
     5341 cacgccaacc agctacatcg ccacctacat cggaaagaac ctggacagcc gcgccgtgga
     5401 tggcatcgac ccgaaaacgg gcaagccacg cgtggaccac gaaaccggaa aatcaatggc
     5461 cgagagcgtg gagcgcgcca tcggctgggc gcgccttcac cgggtccgtc agttccagtt
     5521 ctttggcatc ccctcccgtc aggtgtggcg tgaactgcgc cgccttgcca gccagatggc
     5581 acgcaatccg gaaggcccgc aacggctgaa agatgatgca atggatgcgg tactcgctgc
     5641 cgccgatgcc gggtgttttg ccacctacat tgaaaaacag ggtggcgtgc ttgttccacg
     5701 caaggactac ctgattcgca ccgcctacga cctcgcagat gagctgaacg attacggcga
     5761 acagagcgta cagatttacg ggatctggtc accactcatc ggggaatcct cccgtgtgtg
     5821 cacgcatccg gataactgga agctggtaag acgtaaaccg gaagcggaag acagcgcccg
     5881 cgaaaatggt tttgaccttc agggcggccc tgccgcccct tggactcgtg gcaataactg
     5941 tccccgtgta caggaaacgg acaacaacgg gacagaacag ccggaagaac ggccagcacc
     6001 gtggccgcag ctccctgacg gcgttgaagt gaacgaatgg atgcgctcac tgaaacgaca
     6061 cgaacgccgg gcgctgatgc gttcgcttcg tgacaaacag gcaaaaaaca gcagcgatga
     6121 aatgcagagc tggacacaga gccgcaaaca gcagcggcct ttgcctgata accacgaatt
     6181 actcgctaaa gaatggcggg agtctgctga atctctcggc ctgcatatcg gtgaacaaca
     6241 gatgcagcac ctgttacggg gcggcagtct gtacgttgac ggcagcatca ttgcaccgca
     6301 gggatttgaa attgtacgca aaccggatac ccgcccggac agccgaatca cgcagctctg
     6361 gcagcgcctg agccgtaatc atggcgtaag cagcacggag atccgccata acccggtcgc
     6421 cagctatctg gcacagctgg gggcatcaga ccctgaagcc gccgcacgcc tggcatccac
     6481 acttcagcag ggccagaaca ccatgaaaac acccgttacc gtgctttctg acatgctgcg
     6541 cgccatccgc gacgcagagc acgcacagag aatcagtgaa accactgaac gcgccagccg
     6601 caaagcagac ctgctgcggg gtggcctgac cagtggaaac aaaaaacaga cagaaacggg
     6661 acttacgaat cccgtaaatg agcaaaaaac gcgcagcgat atatgaagcg cgcacaaaac
     6721 aggcaaaaac gggatttcag aatcccgtaa acgattaatt aatcaacata aggaaaagcg
     6781 acatgaaaat ttgtatcgac gacggctcca ccaacatcaa gctggcatgg actgagaatg
     6841 gcgaacgccg caacgccatc agcccgaaca gcttcaagtc ggaatggtct gcgccgttcg
     6901 gtggcatgca gcccgcgaac tacatgcttg atggcgtgcg ctatggtttt gatccggtta
     6961 gcgatcgctt tgtccagacg accgacacgc aataccagta cagcgatgtg aatgtaatcg
     7021 ccattcatca cgcgctggtc aaatcaggca tcacgccaca ggaggtggat gtggttgtca
     7081 ccctgccact gagcgagtat ttcgacacaa acgcacagcc ggacatggcc aacatcaacc
     7141 gcaaaaaagc gaacgttatg cgcccggtgg aataccagaa cggcgaagca ttcactatcc
     7201 gtaacgtgcg ggttatgcct gaatccattc cggctggctt taaagcactg gctgacatga
     7261 gtccgtttga atccctgctg attgtggatt taggcggaac cacgctggat gtggcaaagg
     7321 ttcaagggca actggcaggt atcagccagg tgttttgcga tccacacgta ggcgtttccc
     7381 tgatggccga tgccgtactg tcggtgatgg ccactaacgg tatgcgcacc agtcaccaca
     7441 tcgccaatac cattatcgaa catcgccatg atgaagcctg gctgcgccag cacatccaca
     7501 atgacgcgca ttacgccagc ctgatggcgg ttattcgtga aaaggaagaa acactgaaac
     7561 aacgcgtgat ccgcgcgctg gcggtttttt cgggttacgg gcgggtgatg gttgtcggtg
     7621 gaggggcgga gattgtggca cccgctatcc gcgaagtctg cggagttaat gcgactttca
     7681 tcgcggacgg ggtgccacag tttgctctgg ttaatgggct gtacgcaatg gacaaggagt
     7741 aaaccaatga cgacaccaac cagacggata agtttctatc tgaagcccgc cgccgtcaag
     7801 agtgaacagg aggcgtgcaa ttacctcgat agcctgccag cctccgaacg cagccgcgcg
     7861 caacgcgcgg cctttctggc tgggctagcg ctcataaagc gcaacccagc atttgcctac
     7921 tggatggccg aatggccaga ggacaacctc cccttcaata ccgttagcat caaggaaaca
     7981 taccaggata atcagcctag cggagttgaa tgcaattttt ataaaataaa aaagaatatt
     8041 cagaccttat tcccagaata atctgataat tgtatgtgaa ctaataaaat acagaccaac
     8101 acatcattct ggcacccttg ctaacagagt gccaatgttt caaatcatga tgaaattgcc
     8161 gaagtttttt tatcgtgctg aagtaccgca aatgc
//
LOCUS       DACADF010000185         8171 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.187, whole genome shotgun sequence.
ACCESSION   DACADF010000185 DACADF010000000
VERSION     DACADF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.187"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            25..1191
                     /locus_tag="ISB30_004245"
     CDS             25..1191
                     /locus_tag="ISB30_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP1991813.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     TQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            1234..2550
                     /locus_tag="ISB30_004246"
     CDS             1234..2550
                     /locus_tag="ISB30_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1991814.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVVTFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            2654..3304
                     /gene="fabR"
                     /locus_tag="ISB30_004247"
     CDS             2654..3304
                     /gene="fabR"
                     /locus_tag="ISB30_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAP1991815.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            3304..3663
                     /locus_tag="ISB30_004248"
     CDS             3304..3663
                     /locus_tag="ISB30_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAP1991816.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQDVAE"
     gene            complement(3703..4803)
                     /gene="trmA"
                     /locus_tag="ISB30_004249"
     CDS             complement(3703..4803)
                     /gene="trmA"
                     /locus_tag="ISB30_004249"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAP1991817.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWCQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      4917..5107
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5172..7016
                     /gene="btuB"
                     /locus_tag="ISB30_004250"
     CDS             5172..7016
                     /gene="btuB"
                     /locus_tag="ISB30_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAP1991818.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDHPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDVWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNIGAG
                     VDWQKQSTAPGTAYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGSRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            6961..7818
                     /gene="murI"
                     /locus_tag="ISB30_004251"
     CDS             6961..7818
                     /gene="murI"
                     /locus_tag="ISB30_004251"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP1991819.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
BASE COUNT         1879 a         1927 c         2214 g         2151 t
ORIGIN      
        1 tataagtgaa taaaggaata gccgatgatc gaacactata ttcgtgggtt tgaggaagaa
       61 cttcgcgaga tccgccatca gatccacgaa aacccggagc tgggattaca agaatttaaa
      121 accagtgctt tggttgcgga aaagctgcgc cagtggggct atgaggttga gcaagggctg
      181 gcgacaaccg gcgtggtagc cacgttaaaa gtgggcgacg gagagaaaag tattggcctg
      241 cgggcggata tggatgcgtt gccgatttat gaaaatagtg gcaagccctg ggccagtaaa
      301 catcctggtt taatgcacgc ctgcgggcat gacgggcata ccaccatact gctgggggcc
      361 gcgcgttatt ttgccgaaac ccgccgtttt aacggcacgt tacgccttat cttccagcca
      421 gcggaagaga tgattaacgg cggtgagatc atggttaaag aggggctttt tgatcgtttc
      481 ccctgcgatg tcattttcgg gatgcacaac atgccgggtt tgccggtcgg caagtttttc
      541 ttccagcctg gggcgctgat ggcgtcaatg gatcagttcc atattacggt tcgtggttgt
      601 ggcggacacg gtgcgatccc gcacaaagcc atcgatccag tgctggttgc cgcacatatc
      661 accaccgcat tacaaagcat tgtgtcgcgc aatgtcgatc cgctggaagc ggcggtaatt
      721 accgtcggca gcattgttgc gggtgaggcg gctaacgtca ttcctgacag tgctgagatg
      781 aaaatcagtg tccgctctct tagccgcgat acccgacaac ttcttctgac ccgtattcct
      841 gctcttgcac aaacccaggc tgccagcttt ggcgctacgg ctgaggttac gcatgttaac
      901 ggtacaccgg tattagtaaa tgacgaggag atggcgcgct tcgcctggca ggtggcgtgc
      961 aaaacatttg gtgaagatcg ggctgagttt ggcatcaaac ccctgatggg cagtgaagat
     1021 ttctctttca tgctggaggc ccaacccaaa ggcggtttcc tgttattcgg taatggcgat
     1081 gtcggggaag gttcctgcat ggtgcataac cccggttacg actttaacga tgcaagcctg
     1141 gtcccggcaa gcagctactg gggcgcactg gttgaagcct ggctgcaata aataaaacaa
     1201 cacacaacaa cactttttgg caaggaacat ggaatgacgg cgatcgtaaa tgaaaccgtg
     1261 agtgtgaaga aaaagcccag cggcaggcgg gtgatctttg cctcggcgtt tggcaatgcg
     1321 ctcgaatttt ttgattttgg cgtttacaac ttttttgtgg tctatatcag tacgcttttc
     1381 ttcccaccca gtgccgatca taacgtcgcg ctgttactgg catttgcaac gtttggtgtg
     1441 agctttttta tgcgtccgct cggtgggatt atcgttggtg cctgggccga tcgcttcggg
     1501 cgtaaaccgg cgatggtgtt cactattgct ctgatgagcc tcggtacgct gatgatcggc
     1561 atcgcaccga cgtatgaaac ggcgggctat tggggaaccg caacgctggt gctggcgcgt
     1621 ttgattcagg gcgttgcggc aggtggtgaa gtcggggctt caatgtcgct actggtggag
     1681 tcagctccgg caaatcgtcg tggcttttac agcagttggt cactggcgac tcaaggcctg
     1741 gcaacaactt ttggcggtgt tgtcgcttta ggtttaagcg catggctacc gttcgccaca
     1801 ggttcagaaa ccgtaatggc agaatggggc tggcgtgtgc cgttttttat tggtgtgtta
     1861 ttagcaccta tcggttgctg gttgcgtctg agcctggaaa atgacgtacc tgagcctgtg
     1921 cgcaataaaa aagctgctac cagcgaaagc gcgttttctc tgctcctgca acataaggcg
     1981 actatcgtta atggcgtttt actcgctatt ggtagcacgg tcgcaaccta tatctccctc
     2041 ttttattacg gcacctgggc ggcgaagtat ctggcgatgc ctcaacatta ttctcatgca
     2101 gcgatgttac tggcgggcgt tgttacgttt gtgggtgcgc tgcttgtggg gatgttgtgc
     2161 gattcggtcg ggcgtaaaaa gctgatttta atctcccgtg tgatggtgct tctctgtagc
     2221 tggccgtcat tctggctgtt ggtgaattac ccaagccctg gcatgttgct gacggtggtt
     2281 ttcgtgatgg tcagctttac cacgcttggc ggtgtgccag tgatgttgtt gatctctgaa
     2341 ctgttgccga aacggattcg ggcattgggt tttgcgctgg tttatagcat tggtgtggca
     2401 atctttggcg gttttgctca atatttcgct acgcaatcca tcgtgttgct ggatagtctg
     2461 acggctccgg cgtggtatct gggtggaggc acgttgctgt cgatgctggc gttgttgtac
     2521 gtaaaagaac cggccaaaga attgcagtaa atatgtttta ttgcgttacc gttcattcac
     2581 aacactggag caatccagta tgttcattct ctggtatagt gccagcagta cttttggcaa
     2641 ggattcagac atcgtgatgg gcgtaagagc gcaacaaaaa gaaaaaaccc gccgttcgct
     2701 ggtggaagcc gcatttagcc aattaagtgc tgaacgcagc ttcgccagcc tgagtttgcg
     2761 tgaagtggca cgagaagcag gcattgctcc cacctctttt tatcggcatt tccgcgacgt
     2821 agacgaactg ggtctgacca tggttgatga gagcggttta atgctacgcc aactcatgcg
     2881 tcaggcgcgt cagcgtatcg ccaaaggcgg gagtgtgatc cgcacctcgg tctccacatt
     2941 tatggagttc atcggtaata atcctaacgc cttccggtta ttattgcggg aacgctccgg
     3001 cacctccgct gcgtttcgtg ccgccgttgc gcgtgaaatt cagcacttca ttgcggaact
     3061 tgcggactat ctggaactcg aaaaccatat gccgcgtgcg tttactgaag cgcaagccga
     3121 agcaatggtg acgattgtct tcagtgcggg ggccgaggcg ttggatgtcg gcgtcgagca
     3181 acgtcggcaa ttagaagagc gactggtact gcagctgcga atgatttcga aaggggctta
     3241 ttactggtat cgccgtgaac aagagaaaac cgcaattatt ccgggaaatg tgaaggacga
     3301 gtaatgaaac aagcaaatca agatagaggt acgctgctgc tggcgctagt ggctggctta
     3361 tcgattaatg gtactttcgc agcgctgttt agctccattg taccattttc tgtattcccg
     3421 attatttccc tggtgttgac agtttactgc ctgcatcaac gttatcttaa tcgcactatg
     3481 ccggtaggct tgccgggtct ggcagctgct tgttttattc tcggcgtact gctgtacagc
     3541 acggtagttc gtgcggaata tccggatatc ggttccaact tcttcccggc agtactgtcc
     3601 gtcattatgg tgttctggat tggcgcgaag atgcgtaacc gtaagcagga cgttgctgag
     3661 taatcagcat catgccggat gcaggcgcat ccgacatggg ttttacttcg cggtcagtaa
     3721 tacgccgcac tccatatggt gcgtgtaggg gaactgatca aatagagcca gacgttcgac
     3781 cttgtgcgtc tggcttaatg tttccagatt cttgcataac gtttccggat tgcaggagat
     3841 atacagaata cgtggatacg cctgcaccat tttctcggtt tcactgtcca gaccgctgcg
     3901 cggtgggtcg acaaaaatgg tttcgcactg ataactcttt aagtcgattc cttgcaggcg
     3961 gttaaactcg cgcacaccat tcatcgcctg agtaaattct tctgccgcca tacgaataat
     4021 ttgtacgtta tcaatatggt tagcggcaat gttgtattgc gcggcggcga ctgacggctt
     4081 ggcgatttcg gtggctaata cccgatcaaa attgcgtgcc agcgctaatg aaaagttacc
     4141 gttgccgcag tatagctcca gtaaatcgcc ttttgagcct ttggttacgt ccagcgccca
     4201 ttccagcatc tgaatattca tcgccgcgtt cggttgggta aagctgtttt ctacctgacg
     4261 gtagatcatc tctttccccg cgatcggcag acgttcgtcg atgtaatcct gatccagcgc
     4321 gattttggtt ttcgttgccc gaccaatcag atgcacattc agattctgcg cgcgcagtgc
     4381 atcgcgcagg gcttccgcct gctgacacca ctcatcatcc agctttttat ggtacagcaa
     4441 ggaaacaacc gcttgattgc ttagcgtagt gaggtaatca atctggaaca acttgtggcg
     4501 cagaatggga ttattacgca cacccgcaat catcgccgtc atcaactggt tgataagttc
     4561 actggcggcg gggaagctat ccacacggat acgacttttg gtttgttgat cgaaaatgat
     4621 gtggtacagg tcatcgccat cgtgccagat gcggaactcc gcacgcatcc gataatgact
     4681 gaccggcgag cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa
     4741 acgtaccact ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt
     4801 catgatgtat cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc
     4861 cagatcttga tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt
     4921 agcatccact tgccggtcct gtgagttaat agggaatcca gtgcgaatct ggagctgacg
     4981 cgcagcggta aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag
     5041 tcatcatctc ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg
     5101 catctggttc tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg
     5161 gatgctttac aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt
     5221 ccgcttgggc acaggatacc agcccggata ctctcgtcgt tactgctaac cgttttgaac
     5281 aaccgcgcag cactgtgctt gcgccaacta ctgttgtgac gcgtcaggat atcgaccgct
     5341 ggcagtcgac ctcggtcaat gatgtgctgc gccgtcttcc gggcgtggat atcacccaaa
     5401 acggcggttc aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgt
     5461 tggtgttaat tgatggcgtc cgcctgaatt tggcgggggt gagtggttct gctgatctta
     5521 gtcagttccc tattgcgctt gtccagcgtg ttgaatatat ccgtgggccg cgctccgctg
     5581 tttatggttc agatgcaata ggcggggtcg tgaatatcat cacgacgcgc gatcaccccg
     5641 gaacggaaat ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacac
     5701 agcaacaact gggggataag acacgggtaa cgctgttggg cgattatgcc catactcatg
     5761 gttatgatgt tgtcgcctat ggtaataccg gaacgcaagc gcagccagat aacgatggtt
     5821 ttttaagtaa aacgctttat ggcgcgctgg agcataattt tactgatgtc tggagcggct
     5881 ttgtgcgcgg ctatggctat gataatcgta ccaattatga cgcatattat tctcccggct
     5941 cgccactggt cgatacccgt aaactctata gccaaagttg ggatgccgga ctgcgctata
     6001 acggtgaact gattaaatca caactgatta ccagctacag ccatagcaaa gattacaact
     6061 acgatccgca ttatggtcgt tatgattcat ccgcgacgct cgatgagatg aaacagtaca
     6121 ccgtccagtg ggcaaacaac atcatcattg gccacggtaa tattggtgcg ggtgttgact
     6181 ggcagaagca gagcacggca ccgggcacag cttatgttga ggatggatat gatcaacgta
     6241 ataccggcat ctatctgacc gggctgcaac aagttggcga ttttaccttt gaaggcgcag
     6301 cacgtagcga cgataactca cagtttggtc gtcatggcac ctggcaaacc agcgccggtt
     6361 gggaatttat cgaaggttat cgctttattg cttcctacgg gacatcttat aaggcaccaa
     6421 atcttggaca actgtatggt ttctacggaa atccgaatct cgaccctgag aaaagcaaac
     6481 agtgggaagg cgcgtttgaa ggcttaactg ctggggtgaa ctggcgtatt tccggatatc
     6541 gtaacgatgt cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag
     6601 ggaaagcgcg gattaagggt gtcgaggcga ccgccaattt tgataccgga ccactgacgc
     6661 atactgtgag ttatgattat gttgatgcgc gcaatgcgat tactgacacg ccgttgttac
     6721 gccgtgctaa acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg
     6781 ggattactta tcagtactta ggttctcgtt atgataagga ttactcctct tatccgtatc
     6841 aaacagtgaa aatgggcggg gtgagcttgt gggatcttgc tgttgcatat ccggtcacct
     6901 ctcacctgac agttcgtggt aaaatagcca acctgttcga caaagattac gagacagtct
     6961 atggctacca aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca
     7021 cgtcccaccg tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc
     7081 cggcatctct tgccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat
     7141 ggcgaaaaaa gcgaagtgtt tattgttgag cgagtggtgg aaattgtcac cgcagtgcaa
     7201 gaacgttatc cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt
     7261 cctgcattac gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct
     7321 gctgcacgtc tgacggcaaa tggcattgtc ggattactgg caacccgcgg aactgttaaa
     7381 cgttcttata ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg
     7441 ggctcggcag agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg
     7501 gatgcgctaa aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agatacagtt
     7561 gtattgggtt gcacccattt ccctctacta caagaagaac tattacaagt gctgccagag
     7621 ggaacccggc tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagag
     7681 catgaagccc cggatgcaaa atctgccgat gcgaatattg cattttgtat ggcaatgacg
     7741 ccagaagctg aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa
     7801 ctggcagttt tagattgatt tggttgaatg ttgcgcggtc agaaaattat tttaaatttc
     7861 ctcttgtcag gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa
     7921 acacgccgcc gggtcagcgg ggttctcctg agaatctcaa cagaggaaag caaaaaaatg
     7981 cttgactctg tagcgggaaa gcgtattatg tataccccgc gccgctgaga aaagcgaagc
     8041 ggcactgctc tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct
     8101 tgacgtcgca agacgaaaaa tgaataccaa gtctctgagt gaacacgtaa ttcattacga
     8161 agtttaattc a
//
LOCUS       DACADF010000186         8105 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.188, whole genome shotgun sequence.
ACCESSION   DACADF010000186 DACADF010000000
VERSION     DACADF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.188"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            233..1216
                     /locus_tag="ISB30_004252"
     CDS             233..1216
                     /locus_tag="ISB30_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HAP1991820.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1288..2436
                     /locus_tag="ISB30_004253"
     CDS             1288..2436
                     /locus_tag="ISB30_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein KpsE"
                     /protein_id="HAP1991821.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2460..4136
                     /locus_tag="ISB30_004254"
     CDS             2460..4136
                     /locus_tag="ISB30_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid transporter KpsD"
                     /protein_id="HAP1991822.1"
                     /translation="MKLFKSILLIAACHAAQASATIDINADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQIDPKGNIFLPNVGPVKVAGVSNSQLNTLVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            4146..4886
                     /gene="kdsB"
                     /locus_tag="ISB30_004255"
     CDS             4146..4886
                     /gene="kdsB"
                     /locus_tag="ISB30_004255"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAP1991823.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDPRVEQAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEATEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMQEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4883..6910
                     /locus_tag="ISB30_004256"
     CDS             4883..6910
                     /locus_tag="ISB30_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HAP1991824.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCCIYYDASKPSAL
                     EKLVQDKAGNTALISQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFADLRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLQRNHQQQRNGYLWSP
                     GLTLWKSAILKPFLRTATNRLSYSRRCTAATACVVWGVKGEQQWLAEAQQKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKVILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERNPHAYIVYKPHPDVLVGNCKGDIPAELIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYAGWGLTVDEHHCPRREQKLTLADLIYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIKTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            6945..>8105
                     /locus_tag="ISB30_004257"
     CDS             6945..>8105
                     /locus_tag="ISB30_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP1991825.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFNDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGERVIYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYLLVKTQVNAV"
BASE COUNT         2078 a         1951 c         2104 g         1972 t
ORIGIN      
        1 aggtgggcaa aatttggttc cccttctcgc ctgtaatatt gcagcatctt cgcacctcca
       61 tgagacattg cgacttaata agaaggtgat aagtcctgca tataagcatg gactgaccat
      121 ggttttatat tcattaaaaa ttttgttaca acccattgat ttagcataaa taaattatag
      181 tgggttcggg ttagttgtga ctgtggcatt atttccgtgc aaaggagctg atatgtctga
      241 aagacattta cctgatgacc agagcagtac tatcgatcca tatctaatta cctctgttcg
      301 ccagactctg gcagaacaag gcgcagcatt acaaaacttg tctaaacaac tggattccgg
      361 gcagtaccag cgtgtcctta atttgataat gaactgtaaa gggcacgtta ttctttcggg
      421 aatgggtaaa tcagggcatg tcggtcgtaa aatgtcagcg acgctggcct ctacgggtac
      481 gcctagtttc tttattcatc ctgcagaagc tttccatggc gatctgggca tgattacgcc
      541 ttacgatctt ctgatcctta tttctgccag cggtgaaacg gatgaaatcc tcaagctagt
      601 tccttcactg aaaaatttcg gcaaccgaat tatcgccatt accaataatg gaaattccac
      661 gctggcgaaa aatgctgatg ccgtgctgga actccacatg gcgaatgaaa cctgcccgaa
      721 taatcttgca ccaacaacgt ctaccacgct gacgatggcg atcggcgatg cgctggcgat
      781 tgccatgatc caccaacgca aatttatgcc gaatgatttt gcgcgctatc acccgggcgg
      841 ttcattaggt cgtcgcctgc tgacccgcgt tgctgatgtc atgcagcatg atgttcctgc
      901 ggtacagctg gatgcgtcat ttaaaaccgt gattcaacgt atcaccagcg gatgccaggg
      961 aatggtgatg gtagaagacg cagaaggtgg gctagcgggc attatcaccg acggtgacct
     1021 gcgtcgcttt atggaaaaag aggattctct gacatccgcc acggctgcgc agatgatgac
     1081 acgtgaaccg ctgacgctac cggaagacac catgatcatt gaagcggaag aaaaaatgca
     1141 aaagcaccgc gtctcaacgt tattggtgac caacaaggca aataaagtca ctggccttgt
     1201 gcgcattttc gactaattaa gcaacggggt gcgagaggtt agtctctcat tgagtttgcg
     1261 ccccactgaa actttgataa tcgttacatg ttgataaaag tgaagtctgc cgtatcctgg
     1321 atgcgtgctc gtctgtctgc catctcactg gcagatatcc aaaaacacct ggcgaaaatc
     1381 atcattctgg caccgatggc ggtgctgctg atctatctgg ctatcttcag ccagcctcgc
     1441 tatatgagcg agtcgaaagt cgccattaaa cgctcggatg atttaaacag cggcagcctg
     1501 aattttggtc tgcttctggg tgcctctaac cccagttccg cagaagatgc gttgtatctg
     1561 aaagagtaca tcaactcgcc ggatatgctg gcggcgctgg ataagcaact aaattttcgt
     1621 gaagcgttta gccacagcgg gctcgatttt cttaatcatc ttagcaagga tgaaaccgca
     1681 gaaggcttcc tgaagtacta caaggaccgt atcaacgtct cgtatgacga taaaaccgga
     1741 ttactgaata ttcagacgca gggctttagc ccggaatttg cgcttaagtt taaccaaacc
     1801 gtgctgaaag agtcagagcg ctttatcaat gagatgtcac atcgcatcgc gcgtgaccag
     1861 cttgcctttg cagaaacgga gatggaaaag gcacgccagc gtctggacgc cagcaaagcg
     1921 gaattgctct cttatcagga caacaacaac gttctggatc cacaggcaca ggcacaggcg
     1981 gcgagcacgt tagtgaatac gctgatgggc cagaagatcc agatggaagc ggacctgcgg
     2041 aacttgctga cgtatctgcg tgaggacgcc ccgcaagttg tgagtgcgcg taatgcgatt
     2101 cagtcattgc aggcacaaat tgacgaagaa aaaagcaaaa tcacagcgcc gcagggtgac
     2161 aagctaaacc gtatggcggt ggattttgaa gaaatcaaat caaaagtaga gttcaacacc
     2221 gagctgtaca aactgaccct gacctccatt gaaaagaccc gtgtagaagc ggctcgtaag
     2281 ctcaaggtgc tgtcagtgat cagttcgcca cagttgccgc aggaatcgtc ttttccaaat
     2341 atcccttatt tgatcgcctg ctggttactg gtgtgctgcc tgctgttcgg caccctgaaa
     2401 ctgttgctgg ctgttattga agatcaccga gactaacgct gtcgctgaat gagtttgtga
     2461 tgaaattatt taaatcaatt ttactgattg ccgcctgtca cgcggcgcag gccagcgcga
     2521 ccattgatat taacgctgac ccaaacctga caggagccgc gccgcttacc ggtattctga
     2581 acggacagaa gtcggatacg caaaacatga gcggcttcga caatactcca ccccccgcac
     2641 cgccggtggt aatgagccgt atgtttggtg ctcaactttt caacggcacc agcgcggata
     2701 gcggtgcgac ggtaggattc aaccctgact atattctgaa tccgggtgat agcattcagg
     2761 ttcgcttgtg gggtgcgttc acctttgatg gtgcattaca gattgatccc aaaggtaata
     2821 tttttctgcc aaacgttggt ccggtgaaag ttgccggggt tagcaatagt cagttgaaca
     2881 ctctggttac atcaaaagtg aaggaagtgt accagtccaa cgtcaacgtc tacgcctcct
     2941 tattacaggc gcagccagta aaagtgtacg tgaccggatt tgtgcgtaat cctggtctgt
     3001 atggcggtgt gacgtctgat tcgttactca attatctgat caaggctggc ggcgttgatc
     3061 cagagcgcgg aagttacgtt gatattgtgg tcaagcgcgg taaccgcgtg cgctccaacg
     3121 tcaacctgta cgacttcctg ctgaacggca aactggggct ttcgcagttc gccgatggtg
     3181 acaccatcat cgtcgggccg cgtcagcata ctttcagcgt tcagggcgat gtctttaaca
     3241 gctacgactt tgagttccgc gaaagcagca ttcccgtaac ggaagcgttg agctgggcgc
     3301 gccctaagcc tggcgcgact cacattacga ttatgcgtaa acaggggctg caaaaacgca
     3361 gcgaatacta tccgatcagt tctgcaccag gccgtatgtt gcaaaatggc gataccttaa
     3421 tcgtgagcac tgaccgctat gccggcacca ttcaggtgcg ggttgaaggc gcgcactccg
     3481 gtgaacatgc catggtactg ccttatggtt ccactatgcg tgcggttctg gaaaaagtcc
     3541 gcccgaacag catgtcgcag atgaacgcgg ttcagcttta tcgcccatca gtagctcagc
     3601 gtcagaaaga gatgctgaat ctctcgctgc aaaaactgga ggaagcctca ctttctgccc
     3661 agtcatccac caaagaagaa gccagcctgc gaatgcagga agcacaactg atcagccgct
     3721 ttgtggcgaa agcacgcacc gtggttccga aaggtgaagt gatcctcaac gaatccaata
     3781 ttgattctgt tctgcttgaa gatggcgacg tcatcaatat tccggagaaa acatcgctgg
     3841 ttatggttca tggcgaagtg ctgttcccga acgcggtgag ctggcagaag ggtatgacca
     3901 ccgaggatta catcgagaaa tgtggtggcc tgacgcagaa atcgggtaac gccagaatta
     3961 tcgtcattcg tcagaacggt gcagccgtca acgcagaaga tgtggattca ctcaaaccgg
     4021 gcgatgagat tatggttctg ccgaaatatg aatcgaaaaa cattgaagtt actcgtggta
     4081 tttccaccat cctctatcag ctggcggtgg gtgcaaaagt gattctgtct ttgtaaggag
     4141 ttgaaatgag caaagctgtt attgtcatcc cggctcgtta tggctcatcg cgtctgccgg
     4201 gtaagccact gctcgatatt gttggtaaac cgatgatcca gcatgtttac gagcgtgcgt
     4261 tacaggtggc gggcgttgcg gaagtttggg tggcaacaga cgatccgcgt gttgaacagg
     4321 ccgtgcaggc atttggcggg aaagccatca tgacgcgcaa cgatcatgaa tccggcaccg
     4381 atcggctggt cgaggtgatg cataaagtcg aggcagatat ctacattaac ctgcagggcg
     4441 acgaaccaat gattcgcccg cgggatgtag aaacgctgtt acaaggaatg cgtgatgacc
     4501 ccgcgttgcc agtggcaacg ctatgccacg cgatttctgc cgaagaagcg accgagccaa
     4561 gcacggtaaa agtggtggta aatacccgcc aggatgcgct ttatttcagc cgttcgccta
     4621 ttccgtatcc gcgtaatgct gaaaaagcgc gctacctgaa acacgttggt atttacgctt
     4681 atcgtcgcga tgtgctgcaa aactacagcc agttaccgga gtccatgcag gagcaggcag
     4741 aatcactgga gcagctgcgg ttgatgagcg cggggatcaa catccgcaca tttgaggttg
     4801 ccgcaaccgg tccgggcgtc gacaccccag catgcctgga aaaagtgcgc gccctgatgg
     4861 cacaggaact ggctgaaaac gcatgattgg cattttctcg tccggtatct ggcgtattcc
     4921 gcatctggag aaatttctgg cgcaaccgtg ccagaaactt tctctgctgc gccctgttcc
     4981 gcaagaagtt gatgctatcg ccgtgtgggg acatcgtccc agcgcggcga aaccagtcgc
     5041 catcgccaaa gcagcgggaa aacccgtcat tcgtctggaa gatggatttg tgcgttcgct
     5101 ggatcttggc gtcaatggcg agccgccgct ttctctggtg gtggatgatt gttgcattta
     5161 ctacgatgcc agcaagcctt cagcactgga gaaactggta caagataaag ccggaaatac
     5221 agctctgata agccaggcca gagaagcgat gcacaccatc gtgaccgggg atttgtcgaa
     5281 atataacctg gcacctgcgt ttgtggctga tgagtcagaa cgttcagaca tcgttctggt
     5341 tgtcgatcag acatttaatg atatgtcagt gacgtatggc aatgctggcc cgcatgagtt
     5401 tgctgccatg ctggaagccg cgatggcgga aaatcctcaa gccgaaattt gggtgaaggt
     5461 gcatccggat gtcctggaag gaaagaaaac aggttatttc gctgatctgc gcgccacgca
     5521 acgagtacgt ttgattgccg agaatgtcag cccgcagtcg ctgttgcgac acgtttcccg
     5581 ggtttacgtc gtgacatccc aatacggctt tgaagccttg ctggcaggaa aaccagtaac
     5641 atgtttcggc cagccctggt atgcaggctg gggcttaacc gacgatcgcc atccgcagtc
     5701 cgctttgtta tctgcccgac gcggttctgc cacgctggaa gaactttttg ccgctgcata
     5761 cctgcgttac tgtcgctata tcgacccgct cacgggggcc gcaagcgatc tatttaccgt
     5821 gctgcaatgg ctgcaattac agcgcaacca tcagcaacag cgtaatggct atttatggtc
     5881 gccaggctta acgctgtgga agtcggcaat cctgaaacct ttcttgcgta ccgcaacaaa
     5941 cagactgagt tattcacgcc gttgtactgc ggcgacggct tgcgtggtat ggggtgtaaa
     6001 gggagaacag caatggctag ccgaagcgca acaaaaatca ctgccgttat ggcgaatgga
     6061 agatggtttt ctgcgttcat ccggacttgg ctctgacctg ctgccgccgc tatcgttggt
     6121 actggataaa cgcgggatct actatgacgc cacgcgcccc agcgacctgg aagtcctgct
     6181 taatcacagc cagttaacgc tggcgcagca gatgcgagct gaaaaattac gccagcgact
     6241 ggttgaaagc aaactgagta agtacaacct gggagccgat ttctctctac cagccgaagc
     6301 caaagataaa aaagttatcc tggtgccggg tcaggtagag gacgatgcct ctattaaaac
     6361 aggcactgtc tcgattaaga gcaaccttga gttattacgc acagtacgcg agcgtaatcc
     6421 gcacgcctac attgtttata aaccgcaccc ggatgtactg gtggggaatt gcaagggcga
     6481 tattccggca gaactaattg ctgaactcgc tgattatcag gcactggacg cagatattat
     6541 tcaatgcatt cagcgcgcag atgaagtgca caccatgaca tcattgtccg ggtttgaagc
     6601 gttattacat ggcaagcaag ttcattgtta cggcctgccc ttctatgccg gttggggttt
     6661 aaccgttgat gaacatcact gcccgcgccg cgagcaaaaa ttaacgttag cagatttgat
     6721 ctatcaggcg ttgattgttt atccaaccta tatccaccca acacggctac aacctattac
     6781 ggtagaagaa gctgcggaat atttaatcaa gacgccgcgc aagccgatgt ttattacccg
     6841 aaaaaaagcg gggcgggtaa tacgctatta ccgcaaatta attatgttct gcaaggtcag
     6901 atttggctaa acaatttcat agttgactat tacgcaaatt atttatgcaa ggtaatgcac
     6961 taaccgtttt attatccggt aaaaaatatc tgctattgca ggggccgatg ggaccttttt
     7021 tcaatgacgt cgccgaatgg ttagagtcat taggacgtaa cgctgtgaat gttgtattca
     7081 acggtgggga tcgtttttac tgccgccatc gacaatacct ggcttactac caaacgccga
     7141 aagagttccc cggatggtta cgggatctcc accggcaata tgactttgat accatcctct
     7201 gctttggtga ctgccgccca ttgcacaaag aagcaaaacg ttgggcaaag tcgaaaggga
     7261 tccgctttct ggcatttgaa gaaggatatt tacgtccgca gtttattact gttgaagaag
     7321 gcggagtgaa cgcatattca tcgctaccgc gcgatccgga tttttatcgt aagttaccag
     7381 atatgcctgc gccgcacgtt gagaacttaa aaccttcaac gatgaaacgt ataggtcatg
     7441 cgatgtggta ttacctgatg ggttggcatt accgtcatga gttccctcgc taccgccacc
     7501 acaaatcgtt ttccccctgg tatgaggctc gttgctgggt tcgtgcatac tggcgcaagc
     7561 aactttacaa ggtaacacag cgtaaggtat tgccgaggtt aatgaatgag ctggatcagc
     7621 gttattatct tgccgttttg caggtgtata acgatagcca gattcgtaac cacagcaatt
     7681 ataacgatgt gcgtgactat attaatgaag tcatgtactc attttcacgt aaagcgccga
     7741 aagaaagtta tttggtgatc aaacatcatc cgatggatcg tggtcacaga ctctatcgac
     7801 cattaattaa acggttgagt aaggaatatg gcttaggtga gcgcgtcatt tatgtgcacg
     7861 atctcccgat gccggaattg ttacgccacg caaaagcggt ggtgacaatt aacagtacgg
     7921 cggggatatc tgcgctgatt cacaacaaac cactcaaagt gatgggcaat gccctgtacg
     7981 acatcaaagg cttgacgtat caagggcatt tgcaccagtt ctggcaggcc gattttaaac
     8041 cggatatgaa actgtttaag aagtttcgtg ggtatttatt ggttaagacg caggttaatg
     8101 cggtt
//
LOCUS       DACADF010000187         8078 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.189, whole genome shotgun sequence.
ACCESSION   DACADF010000187 DACADF010000000
VERSION     DACADF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.189"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(45..626)
                     /locus_tag="ISB30_004258"
     CDS             complement(45..626)
                     /locus_tag="ISB30_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2913 family protein"
                     /protein_id="HAP1991826.1"
                     /translation="MRNMEKTGHLAWCALIALLTAREDGLVESESQENLFITRWFAQV
                     KKQRRFSRDVATDVDWILNQGRTLGVRARLRHKLDYLWRSCTGELSEQNDLFRLTYAL
                     ELAKQYDWGYHVLSDSEWSGRNKILPSASVNSICLLKSALDIGFNDDGTQVLPVPARI
                     GGRAEGLNALLKSCGWEAVSNDDHWKLVTISAG"
     gene            complement(653..1243)
                     /locus_tag="ISB30_004259"
     CDS             complement(653..1243)
                     /locus_tag="ISB30_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011839971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991827.1"
                     /translation="MAPSPEQQKLRLPPNVQVRRRGNTYVICYCNNEVGGVVVTETPS
                     GDTQLRPYVKDPVELNKDIVALVAQQMADALIEHAGRQPDGTIPSPPAPIPGTKLLEN
                     TMFPCSRCGRITARLVFAPECASEADMLSLGERLRNDVRYAPYPVWLIGAPDSEDDSV
                     ARHLTLQLAPIRGEVYREHPDKMNSRLMELDDKHHC"
     gene            complement(1258..1551)
                     /locus_tag="ISB30_004260"
     CDS             complement(1258..1551)
                     /locus_tag="ISB30_004260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991828.1"
                     /translation="MKKSLLILGVLSLSISGISQAAGQMNEKALREKAAFALGVTQDR
                     VTLEQSDRTPEPNSYFFNARYQGKLYQCYVGTIQQQIMSDAVCRPTDGSDLPR"
     gene            complement(1640..1924)
                     /locus_tag="ISB30_004261"
     CDS             complement(1640..1924)
                     /locus_tag="ISB30_004261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991829.1"
                     /translation="MDSLTKFALDILRDRNFSRLDEEVREEVLFLFIDDQRKPSKEGR
                     RTLALNAGLLAKQMGEPRLEVLSMDVLMACDKAEVREVLAQITDILQGQA"
     gene            complement(1984..2832)
                     /locus_tag="ISB30_004262"
     CDS             complement(1984..2832)
                     /locus_tag="ISB30_004262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991830.1"
                     /translation="MFRANEEAEKLKAEAINYFLIKEIAPWRKDNIDAISETDRKRAE
                     DALSVICTKLGPVVSSYPEWHPVIALGRDKSIPCYRDTQTTPSFPRLDHTRYMANGII
                     TCPYGDTDELIAAVKRSYWDLMQYLSSDDMRFSSLSGWLRMASDSIELRASYITDELI
                     TAFKNSDFDYDGSDVLSDVSGLIPLYANTAKPVLIWWSWNNHALESDGTIPPAVAVPL
                     MLSRTLADLSYAQLSESWENMRYLLLGSPHGARSSLLLNQLTVKQLRTMFNGLMDSGA
                     FGPKKG"
     gene            complement(3975..4850)
                     /gene="repA"
                     /locus_tag="ISB30_004263"
     CDS             complement(3975..4850)
                     /gene="repA"
                     /locus_tag="ISB30_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490498.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAP1991831.1"
                     /translation="MREGVTDAQTLFSHYRQVKNPNPVFTPREGKKTLPFCRKLMAKA
                     EGFTSRFDFSVHVAFVRSLGKRHRMPPLLRRRAIDALLQGLCFHYDPLANRAQRSITN
                     LAIECGLATESKSGNLSITRATRALKFMAELGLITYQTEYDPQIGCNIPTDITFTPAL
                     FSALDVSDVAVVAARRSRVEWENQQRKKQNLKPLEMDELIAKAWRFVRERFRSYQSER
                     KLHGLKRARARRDSDRTRKDIVTLVKQQLTREYASGRFTGGLDAMKRELERRVKERML
                     MSRDNNYTRLATVPI"
     gene            complement(4831..4905)
                     /gene="tap"
                     /locus_tag="ISB30_004264"
     CDS             complement(4831..4905)
                     /gene="tap"
                     /locus_tag="ISB30_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020323529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAP1991832.1"
                     /translation="MLRKLQYLLCSVLLPCNISAGRCD"
     gene            complement(5139..5396)
                     /locus_tag="ISB30_004265"
     CDS             complement(5139..5396)
                     /locus_tag="ISB30_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAP1991833.1"
                     /translation="MSQVGNAVTSSSKEKRPYRKGQPLSSGERKQRSVSRKKDTHKAV
                     TVFIQNANKHTLDTLCSEYGMTQAELFEFLLEQEELRKLKK"
     gene            complement(5548..6102)
                     /locus_tag="ISB30_004266"
     CDS             complement(5548..6102)
                     /locus_tag="ISB30_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAP1991834.1"
                     /translation="MAVRSKANTCRWLVAGLLGLALYAPAPAAYAASIETGFSPEGTA
                     LQLVLKTIESAQHEIRLMGYSFTSPEVVSALVRAKHRGVDVKIVLDEKGNRNKTSQSA
                     MNVVVNAGIPLRTNGRYAIMHDKVIIVDNHTVESGSFNMTRSAASRNSENVLVIKDVP
                     EVAQAYLQHWQSRWDGGTEWHSSY"
     gene            complement(6114..6320)
                     /locus_tag="ISB30_004267"
     CDS             complement(6114..6320)
                     /locus_tag="ISB30_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991835.1"
                     /translation="MVINQLVPWITVGIFVASVALFCQLLKRDRRAWTSLVAVVVLME
                     ELTLSLWLLINDVQILSPHFMDGM"
     gene            complement(6448..6756)
                     /locus_tag="ISB30_004268"
     CDS             complement(6448..6756)
                     /locus_tag="ISB30_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991836.1"
                     /translation="MIVPVTAERDEQGYWSHPAYARSGCETAAEFSYWLRIHGLQCFV
                     MTMRDEAPEVFAAGFTDEAPDARGWIPEPPDDDGWFLGSVHDTDDGPVCYWFRYTRTS
                     "
     gene            complement(6753..7295)
                     /locus_tag="ISB30_004269"
     CDS             complement(6753..7295)
                     /locus_tag="ISB30_004269"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991837.1"
                     /translation="MRVYKYTGLAFVCCLIYLFAYLAINNVQSEGASLKTCALITMPL
                     FIWMVYQRITAMSEKARARESDEVIFMRMMEQIHHYEELERVAGGVRLMKGILECREV
                     AQAIRRPESEDASLTLSRLAYIEHLLSVLAPGLCRRMSEGERQRWMQMMNEPAPGENT
                     AGECHTETPVSQSVPGEVRG"
     gene            complement(7517..>8078)
                     /locus_tag="ISB30_004270"
     CDS             complement(7517..>8078)
                     /locus_tag="ISB30_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAP1991838.1"
                     /translation="FLLSRVLKQKRPQAPLIRRLREAGVRVGDTEQLMAGGVFWERQA
                     QLMTDREVHFMQGLFRSVDMRRWYLCPQVRVADIVQITPRVRGRSRTWWKLFHMAAQW
                     HCDVVIVDRRTFRVVAAVELDDASHLKKSRCRRDILLDEVMRQAGMPLLRSRDARELQ
                     RMIRDFLTALEAGSGVSDAITQQKAG"
BASE COUNT         1930 a         2277 c         1999 g         1872 t
ORIGIN      
        1 ctgccccctt aagagttgca ttcacgcgac ccgttctttg ctggtcaacc agcgctgatg
       61 gtcaccagtt tccagtgatc gtcattactg acggcctccc agccacagct tttcagcagc
      121 gcattaagcc cctcggcgcg cccgccaatt ctcgcaggca caggtagcac ctgcgtcccg
      181 tcatcattaa aaccgatatc cagcgcgctt ttcagcagac agatactgtt caccgatgcc
      241 gatggcagaa tcttatttcg accgctccac tcactgtcgc tgaggacgtg atacccccag
      301 tcgtactgct tcgccagctc cagtgcgtag gtcaggcgaa aaaggtcgtt ctgctcagac
      361 agttcgccgg tgcagctacg ccagagataa tccagcttat ggcgcagccg cgcacgaacg
      421 ccgagcgtac ggccctggtt gaggatccag tcaacatcgg tggccacgtc acgtgaaaaa
      481 cggcgctgtt ttttcacctg cgcaaaccac cgggtgataa acaggttttc ctgcgactca
      541 gactcaacca ggccatcttc cctggctgtc agcagggcga taagagcaca ccatgccagg
      601 tgacccgttt tttccatatt tctcatatca tcaccagccc gggggctgtc cttcagcaat
      661 gatgtttatc atccagttcc atcagtcggc tgttcatctt gtcgggatgt tcccgatata
      721 cctcgcccct gatcggtgcc agctgtagcg tcagatgccg ggcaacgcta tcgtcctcac
      781 tgtccggcgc accaatcagc cagaccgggt agggggcata cctgacgtca ttccggagac
      841 gttcacccag agagagcata tcagcctcag acgcgcactc cggggcaaaa accagccggg
      901 cagtaattcg cccgcagcgg gagcagggga acatggtgtt ctcaaggagc ttcgtgccgg
      961 gaatgggagc cggtggtgaa ggaatggtac catcaggttg cctgccggcg tgctcaatca
     1021 acgcatcagc catctgttga gcaaccagcg ccacaatatc tttgttcagt tccacagggt
     1081 ctttaacgta cgggcgcagc tgcgtgtcgc cggatggggt ttcggtgaca accacaccac
     1141 ccacctcatt gttacaataa caaataacgt atgtgtttcc ccggcggcga acctgaacgt
     1201 tcgggggcag tcgcaatttt tgctgttctg gcgatggtgc catccgactt ccctgcatta
     1261 acggggtaaa tctgaaccat cggttggacg acagacggcg tcagacatga tctgctgctg
     1321 aatggtgcca acgtaacact gatacagttt cccctgataa cgggcattaa aaaaataact
     1381 gttgggttca ggagtacgat cagattgttc aagcgtgaca cgatcctggg tcacccccag
     1441 cgcaaaagca gccttttccc gcagagcctt ttcattcatc tgacctgcag cctgagagat
     1501 tccgctgatg gaaagcgaca gaacgcccag aataagcagt gattttttca tgattacctt
     1561 tccttttgat taataagggc tgtcagccat cccttcacag caatacagta acgggaaaac
     1621 gttcgctcag gcaataatgc taagcctgtc cctgaagaat gtctgtaatt tgtgccagaa
     1681 cctcacgtac ttccgctttg tcacaggcca taagtacatc cattgaaagc acctccagtc
     1741 gtggctctcc catttgcttt gccagcaggc cagcattaag tgccagcgtt ctccgcccct
     1801 cttttgatgg tttacgctga tcatcaataa acaggaacag cacttcttcc ctgacctcct
     1861 catccagccg ggaaaagttt cggtcacgta aaatatccag agcaaatttc gttaaagaat
     1921 ccatatattc tcctggttgt tgaggtactg aacacccgaa actcaaaaaa agcggcttgt
     1981 ccttcatcct tttttcggcc caaaagcccc ggaatccatt aatccattaa acattgtgcg
     2041 taactgctta accgttagct ggttcagaag tagcgacgac cttgccccat gaggagaccc
     2101 gagaagcagg taacgcatat tttcccatga ctctgacaac tgggcatatg acagatccgc
     2161 cagcgtcctg gacaacatta aaggaaccgc caccgcagga ggtatcgtgc catcagattc
     2221 aagggcatga ttattccagc tccaccagat tagcactggt tttgctgtat tcgcatacag
     2281 agggatcaag cccgatacat cagaaagaac atcgctccca tcgtagtcaa aatcactatt
     2341 tttaaacgcg gtaatgagtt cgtcagtgat ataagaagcc cgcaactcga tggagtccga
     2401 tgccatacgc aaccatccgg aaaggctact aaaacgcatg tcatcactgc tcagatattg
     2461 cattaaatcc cagtaggaac gctttacagc agcgattagc tcatcagtat ccccataggg
     2521 gcatgtaata ataccgttag ccatataacg cgtatgatca aggcgcggaa atgatggcgt
     2581 cgtctgcgtg tccctgtaac agggtatcga cttatcacgg ccaagcgcta taactggatg
     2641 ccattcagga taagaagaca ctacaggccc aagtttcgtg caaataacgc ttaaagcatc
     2701 ctcagctcgt ttcctgtcag tttcagagat agcgtcgata ttatcctttc gccatggtgc
     2761 gatctcttta ataagaaagt aattgattgc ctcagctttg agtttttcag cttcttcatt
     2821 tgctcgaaac ataaccatac cccaacataa cattattaac ttacgcatta tctataacac
     2881 atacgcgtaa gtaaatggct ttatgtttac aataaaaagg tacggctact tttgcacaat
     2941 cacctgcctg catggaggca ggtgcgactg atgaacagga tactgacggt ctgtggtatc
     3001 gatacccggc atagccgtaa acacaacttc tatattcgta ttgtgaatgc cgagcagcgc
     3061 gcaggcattc taggaatgcg ccaggggcgc atagaggcgc cctcagaagt ggactagagc
     3121 atagtggtta atggcgccgt agcccggcgt agccggttac ggtgttgata ggttccacca
     3181 atccatccct tcgccctgct agaccaaaac ccgctaaaag cgatacagaa gcttttgtgc
     3241 ggcgtcggag tggtcacgac aaggcggaag ccgcagtcgg ggcgtatctc cgcgttagcg
     3301 ggccgtgagg gccgcttacg ggcgtgtact caaaacttcc agcgacacca ctcatagcca
     3361 ctcagaaatc tagttacaac atccatcatt aaaagcgacg ttgttccggg cctttggccc
     3421 ggggcggcct cgcttttcag tggtggggag gggctgtgag ggtccaggca ggcactgttg
     3481 ctgcttttga gtgagtattt tgaatgttgt aactatatgt attgtgtcgg ggcccgtcgc
     3541 gtagcgatgg gagctactta aggtagttgc cgaaccggcg cggacatagt ccgctgcaag
     3601 ggcgggcatt ctcaggagcc tgtccacagg tcaggcgacg aacaggttat atatttatat
     3661 gaatgaaata aaagatattt aaaagaagta cagagcaggt ttttaaagga aaccacgcac
     3721 tttgaaatca ggtcggctgg cgctctgtaa gcataacggt tgtaacctta tggaaaggtc
     3781 ccttgcagtt atctgtgggt aactgaatct ggcggccaga tgtggccact ctgatgcaat
     3841 ttctggccgc tttgatgccg gaatggggtg aatgtcagga atgacggccg tcatttcgtg
     3901 cagaaaaccg tcagtcggga gctgcagacg tgcgaaaccc tccggcgcca gccggatttt
     3961 tcacacagga agggttatat ggggacggtg gccagccgcg tgtagttgtt atcacgcgac
     4021 atcagcatgc gctctttcac gcgccgctcc agctcccgct tcatggcatc aagaccgccg
     4081 gtgaaccgcc cgctggcgta ttcccgcgtc agctgttgct tcaccagtgt cacaatgtcc
     4141 ttacgggttc tgtccgagtc acgacgtgca cgggcgcgtt tcaggccatg tagcttacgc
     4201 tcggactggt aactgcggaa ccgttcgcgc acaaaacgcc aggccttcgc tatcagctcg
     4261 tccatctcca gtggcttcag gttctgcttc ttacgctgct gattttccca ttcgacgcgg
     4321 ctgcggcgtg cagccacaac agccacgtca gagacatcga gcgccgagaa cagtgccggc
     4381 gtgaaggtga tatctgtcgg tatattgcag cctatctgcg ggtcatactc ggtctggtag
     4441 gtaatcagcc ccagctccgc cataaacttc agtgcccgtg tggcacgggt gatagacaga
     4501 ttgccacttt ttgactcggt ggccagaccg cactcgatgg ccagattggt aatagagcgc
     4561 tgtgcacgat tggccagcgg gtcatagtgg aagcacaacc cctggagcag cgcatcgatg
     4621 gctcgacgac gcaacaacgg cggcatgcga tgacgtttac ccagcgagcg aacgaacgcc
     4681 acatggacgg aaaagtcaaa acgggacgtg aagccctcag ctttcgccat cagcttacga
     4741 cagaacggca gggtcttttt cccctcgcgc ggcgtgaata ccggattcgg gtttttgacc
     4801 tgacgataat gggagaacag ggtctgagcg tcagtcacac cttcccgcac ttatgttgca
     4861 cggaaggagc actgagcaca gaaggtattg cagttttctg agcatgcaca tatactcccc
     4921 gtacagagca acagcttctg tacggtttag ttagagcccc ggtaatcttc ttcgtcgcca
     4981 aacatagaaa gattatcggg gtttcgtctt ttctggttcc gggtagacac ctcatcagaa
     5041 ccagttccct gccaccttgc ggcgtggcca gccatgaaat acatcagacg atcagtaatc
     5101 tagcactgag gtacttctta agcaacaaat gtgacttgtt acttttttag tttacgcagt
     5161 tcttcctgtt caaggaggaa ctcaaataac tcagcctgag tcataccgta ctcggagcaa
     5221 agagtatcaa gcgtgtgctt attagcattc tgaatgaata ccgtaacagc tttatgcgta
     5281 tcctttttac gggataccga acgctgtttg cgttcaccgg atgacaatgg ttgccctttt
     5341 cgataaggcc tcttttcttt cgatgaggaa gttactgcat ttccgacctg cgacatattt
     5401 gcctcctcaa ggatcaagca aggatcgttt gcagaggata ctacagattc gtgaaatcag
     5461 cgacttaact ttacgcgaaa gagtgagcca tgttggcgca tgactcacct gaatggatgt
     5521 agattgcaga tcacattacg atgctgatta gtatgaagaa tgccactccg ttcccccgtc
     5581 ccagcgcgac tgccagtgct gaaggtaggc ttgcgccact tccggcacgt ccttaatcac
     5641 cagcacattc tcgctatttc tcgaggccgc agagcgggtc atattaaagc tgcctgactc
     5701 aaccgtatga ttatcaacaa taatgacttt atcgtgcatg atggcgtagc gaccgttggt
     5761 acgcagcgga ataccggcat tcaccacaac attcatcgcc gactggctgg ttttgttccg
     5821 gttccctttc tcatcgagca ctatcttcac atccacaccc cggtgttttg ctctgacaag
     5881 tgcgctaacc acctcaggag acgtaaatga gtagcccatc agccgaattt cgtgctgtgc
     5941 tgactcaata gtcttaagga ccagctgcag tgccgttcct tccggagaaa aacccgtctc
     6001 gatgctggcg gcatacgctg ccggtgcggg ggcataaagg gccagaccga gcagccctgc
     6061 caccaaccat ctgcaggtat ttgctttgct gcgaactgcc atagttatcc tcattacata
     6121 ccgtccataa agtgcgggga gagtatctgt acgtcattaa tcagcagcca gaggctcagg
     6181 gtcagctctt ccatcagaac cacaacagca accagtgacg tccacgcccg ccgatcgcgt
     6241 ttcaggagct gacaaaacag cgccacgctg gccacaaaaa tgccgacagt tatccaggga
     6301 acgagctggt tgataaccat tgcactgctg ctgcactcaa aacatgccat aggatttcct
     6361 tcacagaatt aattaagaaa cgccgggagc gggggaaact cgctttgccc cggttccggg
     6421 gttggacaac gacgcggagc ggaggcgtca ggacgttctg gtataacgga accagtagca
     6481 gacaggcccg tcgtcggtat catggactga gcccagaaac cagccgtcat catccggcgg
     6541 ttcgggtatc cagccccgtg cgtccggcgc ttcatcggta aatccggcag caaaaacctc
     6601 aggagcctca tcgcgcatgg tcattacaaa gcactgcagc ccgtggatcc tcagccagta
     6661 gctgaactcc gccgcggttt cgcagccact gcgggcatac gccggatgcg accagtaccc
     6721 ctgctcatca cgctctgcgg taacaggcac aatcatcctc tgacctcccc cggcacggac
     6781 tgactgacgg gcgtttcggt gtggcattca cctgccgtgt tttcaccggg cgccggttcg
     6841 ttcatcatct gcatccatct ctggcgctca ccttccgaca tcctccggca cagccccggt
     6901 gccaggacac tcagcaggtg ctcaatgtac gccagccggc tcagcgtcag ggatgcgtct
     6961 tcggactccg ggcgccttat cgcctgagcc acctcacgac actcaagaat acctttcatg
     7021 agccgcacgc caccggccac gcgctccagc tcttcgtaat gatgtatctg ctccatcatt
     7081 ctcatgaaaa tgacctcatc gctttcacgg gcacgcgctt tttcactcat cgcagtaatg
     7141 cgctgataaa ccatccagat aaataacggc attgttatca gcgcacatgt tttcaggctg
     7201 gccccctccg actgaacgtt atttattgcc agataagcaa acagataaat caggcagcag
     7261 acaaatgcca gcccggtata tttataaacc cgcatagttc accttttaaa tttaaccttt
     7321 aagttcagac ataaagccca cattgaaccc ccggaaaagt caataaacag aaaaaccagg
     7381 aatggcatta tttcccgtga attaacgtta tttatcggaa actttaatta acgcagtcat
     7441 ctctcattaa ggagtgagtt ttattctgcg ccgggtcaga tggagaatga attaatactg
     7501 cattgcgcca ggtagcttaa cccgcttttt gttgagtgat ggcatctgac accccggacc
     7561 cggcctcaag tgccgtcaga aagtcccgta tcatcctctg cagctcccgg gcatcacggc
     7621 ttcgcagcag cggcatgccc gcctggcgca tgacctcatc cagcaggatg tcacgccggc
     7681 agcggctttt cttcaggtgg gacgcatcgt ccagctccac ggccgccaca acgcgaaacg
     7741 tcctgcggtc aacgataacc acgtcgcagt gccactgggc cgccatatgg aaaagcttcc
     7801 accacgtccg gctccggccc ctgacgcgcg gcgttatctg cacgatatca gccaccctga
     7861 cctgcgggca cagataccac cgcctcatgt ccaccgaccg gaacagcccc tgcataaagt
     7921 gcacctcacg gtcggtcatc agctgcgcct gccgctccca gaacacgccg cccgccatca
     7981 gttgctctgt atcgcccacc ctgacgccag cctctctcag ccgcctgata agcggtgcct
     8041 gcggccgttt ctgcttcagc acgcgggaga gcaggaaa
//
LOCUS       DACADF010000188         7848 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.190, whole genome shotgun sequence.
ACCESSION   DACADF010000188 DACADF010000000
VERSION     DACADF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.190"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(197..1228)
                     /gene="rsmC"
                     /locus_tag="ISB30_004271"
     CDS             complement(197..1228)
                     /gene="rsmC"
                     /locus_tag="ISB30_004271"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAP1991839.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLNRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANSVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1331..1744
                     /gene="holD"
                     /locus_tag="ISB30_004272"
     CDS             1331..1744
                     /gene="holD"
                     /locus_tag="ISB30_004272"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAP1991840.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPPHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VTSPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1713..2159
                     /gene="rimI"
                     /locus_tag="ISB30_004273"
     CDS             1713..2159
                     /gene="rimI"
                     /locus_tag="ISB30_004273"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP1991841.1"
                     /translation="MNTISSLETTDLPAAFHIEQRAHTFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2174..2851
                     /gene="yjjG"
                     /locus_tag="ISB30_004274"
     CDS             2174..2851
                     /gene="yjjG"
                     /locus_tag="ISB30_004274"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAP1991842.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGVITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            2942..4531
                     /gene="prfC"
                     /locus_tag="ISB30_004275"
     CDS             2942..4531
                     /gene="prfC"
                     /locus_tag="ISB30_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP1991843.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4924..5529
                     /gene="osmY"
                     /locus_tag="ISB30_004276"
     CDS             4924..5529
                     /gene="osmY"
                     /locus_tag="ISB30_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAP1991844.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5656..5817
                     /locus_tag="ISB30_004277"
     CDS             5656..5817
                     /locus_tag="ISB30_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAP1991845.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            5939..7012
                     /locus_tag="ISB30_004278"
     CDS             5939..7012
                     /locus_tag="ISB30_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAP1991846.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTCHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7009..7791
                     /locus_tag="ISB30_004279"
     CDS             7009..7791
                     /locus_tag="ISB30_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP1991847.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1887 a         2026 c         2124 g         1811 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcga cagaggtgta attgctggaa aaatgtccat cagcggattg
      121 tggataattc gtttgtcggt cagataaggc gtgtacgccg catccaacac ggtatgcggc
      181 gacagatatc agatacttaa cctttcttcg cctggcgggt cataatggcg cgatacacct
      241 tgaagcgccc ggtttgcgca atcacttcgt ggaaaccaaa tgtctcatcc agcacgtccg
      301 ggtaaggcag gaaggcgttc gctacaattc gcagctcgcc gccgctatta agatgacgca
      361 ccgcgccgcg aatcagcgtt tgcgccgcat ccaggctggt ttgcatccca tcgtggaacg
      421 gcgggttgga gatgatcata tcaaaacgac ctttcacctc ggaaaagacg ttgctggcaa
      481 agacttcacc ttcaacactg ttggcagcaa gtgttgcgcg actggcttct accgctggtg
      541 cagaaacatc acacagagtg aggcggatct tcggtgaatg gcgcgcaaaa gcaacggaaa
      601 gaacgcctgc gccgcagccg acatccagca ctttaccttt cgtgtgcggg gttagcgtcg
      661 agagcaacaa ctgactgccg acatccagac cgtcgcggct aaacacgccc ggcagcgttt
      721 tgaccgtcaa gccatcgacg ctgtattcgc cccagaattt attggcatca aataccggct
      781 gcttttccag acgaccaaaa tagaggccgc agcgacgggc gctgtctacc ttattcaacg
      841 gcgcataatc ggccagcatc tgctcggcgc tgcgcacgcc gctgcggttc tcgccaacga
      901 caaaaatatc cgtgcccacc ggaagcagag aaagtaaatt catcaactgg aactgggctt
      961 ccggtttatt cttcggccag tagtaaatca aagtatcgca atcagcgaca tcatccgccg
     1021 tggcgaccag gctaaaacgg gcgttatccc ccatctggcg gtttaacacc tgccagtggt
     1081 ggaattgctg ggtatgagca cggctggccg cggtatctaa acgcgcgggc aggtcatcct
     1141 gtaagtctcc ggcaaacaga atacggcttt gttcgaaatc atcactgtga cgcagcaaga
     1201 cttcacttgc cggggtaaat gcagacatgg aatgctcctc aattgatact ggcggcgatt
     1261 atagccatat gttggcgcgg tatcgacgaa tttgctatat ttgcgcccct gacaacagga
     1321 gcgattcgct atgacatccc gacgagactg gcagttacag caactgggca ttacccagtg
     1381 gtcgctgcgt cgccctggcg cgttgcaggg ggagattgcc attgcgatcc cgccacacgt
     1441 ccgtctggtg atggtggcaa acgatcttcc cgccctgacc gatcctctgg tgagcgatgt
     1501 tctgcgtgcg ttaaccgtca gccccgatca agtgctgcaa ctgacgccag aaaaaatcgc
     1561 gatgctgccg caaggcagcc gctgcaacag ctggcggttg ggtactgacg aaccgctatc
     1621 actggaaggc gctcaggtga catcaccggc gctcaccgaa ttacgggcaa acccaacggc
     1681 acgcgccgcg ttatggcaac aaatttgcac atatgaacac gatttcttcc ctcgaaacga
     1741 ctgatttacc agcggctttc cacattgaac aacgcgccca cacctttccg tggagtgaaa
     1801 aaacctttgc cagcaaccag ggcgagcgct atctcaactt tcagttaacg caaaacggca
     1861 aaatggcggc gtttgcgatt acgcaagtgg tgctggatga agctacattg ttcaatattg
     1921 cggtcgatcc tgactatcag cgtcagggac tgggaagggc gctgctggaa catctgatcg
     1981 acgaactgga aaaacgcggc gtggcgacgc tatggctgga agtccgtgcc tcaaatgctg
     2041 ccgccattgc cctgtacgaa agtttaggct ttaacgaggc gacgattcgc cgcaattact
     2101 accccaccac ggacggtcgc gaagacgcca tcatcatggc gttaccaatc agtatgtaag
     2161 acaaggtgga ataatgaagt gggactggat tttctttgat gccgatgaaa cgctgtttac
     2221 ctttgactca ttcaccggcc tgcagcggat gtttcttgat tacagcgtca cctttaccgc
     2281 tgaagatttt caggactatc aggccgttaa caagccactg tgggtggatt atcaaaacgg
     2341 cgtgatcact tcattacagc ttcagcacgg gcggtttgag agctgggccg aacggctgaa
     2401 cgtcgaaccg ggtaaactca acgaggcctt tattaatgcg atggcggaaa tctgtacgcc
     2461 gttgccgggg gcggtttccc tgcttaacgc cattcgtggc aacgctaaaa tcggcatcat
     2521 caccaacggc tttagcgcct tgcagcaagt gcgtctggaa cgcacgggcc tgcgtgatta
     2581 cttcgatttg ctggtgattt ccgaagaagt tggcgttgcc aaaccgaata agaaaatttt
     2641 cgattatgcg ctggaacagg cgggcaatcc tgaccgttca cgcgtgctga tggttggcga
     2701 cactgccgag tccgatattc tcggtggcat caacgccggg cttgcgacct gctggctgaa
     2761 tgcgcaccat cgcgagcaac cagaaggcat cgcgcccacc tggaccgttt cttcgttgca
     2821 cgaactggag cagctcctgt gtaaacactg attgcctccc ccccgttgat gggtaaaata
     2881 gccgcaattt ttcgttttca acaagcgcgg cgcgatgccg cttactcaag aagaaagaat
     2941 tatgacgttg tctccttatt tgcaagaggt ggcgaagcgc cgcacttttg ccattatttc
     3001 tcacccggac gccggtaaga ctaccatcac cgagaaggtg ctgctgttcg gacaggccat
     3061 tcagaccgcc ggtacagtaa aaggccgtgg ttccaaccag cacgctaagt cggactggat
     3121 ggagatggaa aagcagcgtg ggatctccat tactacgtct gtgatgcagt ttccgtatca
     3181 cgattgcctg gttaacctgc tcgacacccc ggggcacgaa gacttctcgg aagataccta
     3241 tcgtaccctg acggcggtgg actgctgtct gatggttatc gacgccgcaa aaggtgttga
     3301 agatcgtacc cgcaagctga tggaagttac ccgtctgcgc gacacgccga tcctcacctt
     3361 tatgaacaaa cttgaccgtg atatccgcga cccgatggaa ctgctcgatg aagttgagaa
     3421 cgagctgaaa atcggctgcg caccgatcac ctggccgatc ggctgcggca agctgtttaa
     3481 aggcgtttac cacctttata aagacgaaac ctatctctat cagagcggta aaggccacac
     3541 cattcaggaa gtccgcattg ttaaagggct gaataacccg gatctcgatg cagcggtcgg
     3601 tgaagatctg gcacagcagc tgcgtgacga actggaactg gtgaaaggcg cgtctaatga
     3661 gttcgacaaa gagctgttcc ttgcgggcga aatcactccg gtattcttcg gtactgcgct
     3721 gggtaacttc ggtgtcgatc atatgctgga tggcctggtg gagtgggctc ctgcgccgat
     3781 gccgcgtcag actgataccc gtaccgtaga ggcgagcgaa gacaaattta ccggcttcgt
     3841 atttaaaatt caggccaaca tggacccgaa acaccgcgac cgcgtggcgt ttatgcgcgt
     3901 ggtatccggt aaatatgaaa aaggcatgaa gctgcgccag gtgcgcactg cgaaagatgt
     3961 ggtgatctcc gacgcgctga cctttatggc aggtgaccgt tcgcacgttg aagaagcata
     4021 tccgggcgat atcctcggcc tgcacaacca cggcaccatt cagattggcg acacctttac
     4081 ccagggtgag atgatgaagt tcaccggtat tccgaacttc gcgccagaac tgttccgtcg
     4141 catccgcctg aaagatccgc tgaagcaaaa acagctgctc aaagggctgg tacagctttc
     4201 cgaagagggc gcggtgcagg tgttccgtcc aatctccaac aacgatctga tcgttggtgc
     4261 ggttggtgtg ctgcagtttg atgtggtggt atcgcgcctg aagagcgaat acaacgttga
     4321 agcagtatat gaatcagtca acgttgccac cgcccgctgg gtagaatgtg cagacgcgaa
     4381 gaaattcgaa gagttcaagc gtaagaacga aagccaactg gcgcttgatg gcggcgataa
     4441 cctcgcttac atcgctacca gcatggtcaa cctgcgcctg gcgcaggaac gttatccgga
     4501 cgttcagttc caccagaccc gcgagcatta attcttgcct ccagggcgcg gtagccgctg
     4561 cgccctgtca atttcccttc cttattagcc gcttacggaa tgttcttaaa acattcactt
     4621 ttgcttatgt tttcgatgat attccgagcg gtttcaaaat tgtgatctat atttaacaaa
     4681 gtaatgacat ttctgacggc gttaaatacc gttcaatgcg tagatatcag tatctaaagc
     4741 cgtagattgt cattctaccg atattaataa ctgattcaga ggctgtaatg gtcgttattc
     4801 atcacccatc gcttttgtga tggcgaccat tgacttctgt agagggtgaa gtctctccct
     4861 attcagcaat gcaacctcgt gttgccaggc tcaaattacg agcaaactta caggaataaa
     4921 tcgatgacta tgacaagact gaagatttcg aaaactctgc tggctgtaat gttgacctct
     4981 gccgtcgcga caggctctgc ctacgcggaa aacaacgcgc agactaccaa tgaaagcgca
     5041 gggcaaaaag tcgatagctc tatgaataaa gtcggtaatt tcatggatga cagcgccatc
     5101 accgcgaaag tgaaggcggc cctggtggat catgacaaca tcaagagcac cgatatctct
     5161 gtcaaaaccg atcaaaaagt cgtgaccctg agcggtttcg ttgaaagcca ggctcaggcc
     5221 gaagaggcag tgaaagtggc gaaaggcgtt gaaggggtga cctctgtcag cgacaaactg
     5281 cacgttcgcg acgctaaaga aggctctgtg aagggctacg cgggtgacac cgccaccacc
     5341 agtgaaatca aagccaaact gctggcggac gatatcgtcc cttcccgtca tgtgaaagtt
     5401 gaaaccaccg acggcgtggt tcagctctcc ggtaccgtcg attcccaggc acagagcgac
     5461 cgtgctgaaa gtatcgccaa agcggtagat ggtgtgaaaa gcgttaaaaa tgatctgaaa
     5521 actaagtaat tcgtcgtaat tcgtcctccc gaactttgtc gggaggcgcg atgtgcacca
     5581 cactaaaaat atcgcattga gtgattttca cgctcatatc aagcggtgac attaactatg
     5641 gtaaaggaga cgcttatgtt tcgttggggc atcatatttc tggttatcgc gttaatcgcc
     5701 gccgcacttg ggtttggtgg tctggccggt accgctgcag gcgcagctaa aattgtcttt
     5761 gtcgtcggga ttattctgtt cctggtgagt ttgttcatgg gccgaaaacg accctagatt
     5821 tcagatcatt aagaaaattt tatccaaagc cagtccagcg gactggcttt tgcggtttta
     5881 gcgaataata aattgcgtta ctttgtcatt ctttcacaac aaaaacagga aggcagaggt
     5941 ggggcagcga atacctgtaa cgcttggtaa tattgcgccg ttgtcgctaa ggccgttcca
     6001 gcctggacga atagctctgg tgtgcgaagg cggcggacag cgtggaattt tcacggctgg
     6061 cgtgctggat gagtttatgc gcgcgcagtt taatcctttc gatctttatc tcggcacatc
     6121 tgccggggcg cagaacctct cggcgtttat ctgcaatcag cccggttacg cgcgcaaagt
     6181 catcatgcgc tataccacaa aacgcgaatt tttcgatcca ttgcgctttg tccgtggagg
     6241 aaatcttatc gatctcgact ggctggtgga ggccaccgca agccagatgc cgttgcaaat
     6301 ggataccgcc gcgcggttgt ttgacagcgg caaatcgttt tatatgtgcg cctgtcgtca
     6361 ggatgactac gcgcctaatt actttttacc aaccaaacaa aactggctgg atgtgattcg
     6421 cgcctccagt gcgatacctg gcttttatcg tagcggagtg tcgctggaag gcattaacta
     6481 cctggatggc gggatcagtg atgcgattcc ggttaaagag gcggcaaggc agggcgctaa
     6541 aacgttggtc gtcatccgca ctgtgccgtc acaaatgtac tacacgccgc agtggttcaa
     6601 acgcatggaa cgctggctgg gtgacagtag cctgcagccg ctggtcaatc tggtgcagca
     6661 tcatgaaacc agctatcgtg acattcagca atttattgag aaaccaccgg gcaagctgcg
     6721 gatattcgaa atttatccac cgaagccatt acatagtatc gcgcttggca gtcggattcc
     6781 ggcgctgcgt gaagactata aacttgggcg tttatgcggt cgttatttcc tcgccacggt
     6841 tggcaagcta ttaactgaaa aagcgccgct tacctgccat ctggtgccag tggtgacgcc
     6901 ggaatcgatc gttattccgc ctgcgccagt cgccaacgat acgctggttg ccgaagtgag
     6961 cgatgctccg caggcgaacg acccgacatt taacaatgag gatctggctt gatttgccgt
     7021 tttatcgata cccactgcca ttttgatttc ccgccgttta gtggcgatga agaggccagc
     7081 ctgcaacgcg cggcacaagc gggcgtaggc aagatcattg ttccggcgac agaggcggaa
     7141 aattttgccc gcgtgcaggc gttagcggaa aaatatcaac cgctgtatgc cgcattgggg
     7201 ttgcatccgg ggatgttgga aaaacatagc gatgtgtctc ttgatcagct acagcaggcg
     7261 ctggaaaggc gtccggcgaa ggtggtggcg gtgggggaga tcggtctgga tctcttcggc
     7321 gacgatccgc aatttgagag gcagcagtgg ttactcgacg aacagctgaa actggcgaaa
     7381 cgctacgatc tgccggtgat ccttcattcc cggcgtacac acgacaaact ggcgatgcat
     7441 cttaaacgtc acgatttacc gtgcactggc gtggttcacg gtttttccgg cagcctgcaa
     7501 caggccgagc ggtttgtaca gctgggctac aaaatcggcg taggcggtac tatcacctat
     7561 ccacgcgcca gtaaaacccg cgatgtcatc gcaaaattac cgctggcatc gttgttgctg
     7621 gaaaccgacg cgccggatat gccgctcaac ggctttcagg ggcagcctaa ccgaccggaa
     7681 caggctgtgc gggtgtttga cgtgctttgc gagttgcgcc cggaaccaga agatgagatt
     7741 gccgaagtgt tgcttaataa tacgtatgcg ttgtttagcg ttagtgggta gtttaccctt
     7801 cgtaggcagc atccggcagt cggcgcataa tgcctgatgc gacgcttg
//
LOCUS       DACADF010000189         7845 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.191, whole genome shotgun sequence.
ACCESSION   DACADF010000189 DACADF010000000
VERSION     DACADF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.191"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            94..183
                     /gene="kdpF"
                     /locus_tag="ISB30_004280"
     CDS             94..183
                     /gene="kdpF"
                     /locus_tag="ISB30_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAP1991848.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            183..1856
                     /gene="kdpA"
                     /locus_tag="ISB30_004281"
     CDS             183..1856
                     /gene="kdpA"
                     /locus_tag="ISB30_004281"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAP1991849.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1879..3927
                     /gene="kdpB"
                     /locus_tag="ISB30_004282"
     CDS             1879..3927
                     /gene="kdpB"
                     /locus_tag="ISB30_004282"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HAP1991850.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAVADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3936..4508
                     /gene="kdpC"
                     /locus_tag="ISB30_004283"
     CDS             3936..4508
                     /gene="kdpC"
                     /locus_tag="ISB30_004283"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HAP1991851.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPVLDKQIAARV
                     AALRAANPDASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVNYIGQPVVNVVELNLALDKLDE"
     gene            4501..7185
                     /gene="kdbD"
                     /locus_tag="ISB30_004284"
     CDS             4501..7185
                     /gene="kdbD"
                     /locus_tag="ISB30_004284"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HAP1991852.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHIEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7182..>7845
                     /gene="kdpE"
                     /locus_tag="ISB30_004285"
     CDS             7182..>7845
                     /gene="kdpE"
                     /locus_tag="ISB30_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAP1991853.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGY"
BASE COUNT         1608 a         2071 c         2333 g         1833 t
ORIGIN      
        1 ctttttttac accacgcccg cagatttttg cgaaatcttt gcagccagaa ctctaccctt
       61 ccggtatcac ttttaggcca ctggaggtgc actgtgagtg caggcgtgat aaccggcgtg
      121 ttgctggtgt ttttattact gggttatctg gtttatgccc tgatcaatgc ggaggcattc
      181 tgatggctgc gcaagggttc ttactgattg ccacgttttt actggtatta atggtgctgg
      241 cgcgtccctt aggtagcggg ctggcgcggc tgattaatga tattcctctt cctggtacga
      301 cgggcgttga acgcgtactc tttagcgcgc ttggcgtctc tgaccgcgag atgaactgga
      361 agcagtatct ctcagccatt cttggcctga atatactggg gctggcggtg ctgtttttta
      421 tgttgctcgg tcagcactat ctgccgctta atccacagca gttgccaggg ctgtcgtggg
      481 atctggcgct caataccgcc gtcagctttg tcaccaatac caactggcaa tcttatagcg
      541 gtgaaaccac gttgagctat ttcagccaga tggcgggctt aacggtacaa aactttcttt
      601 ctgccgccag cgggattgcg gtgatttttg ccctcattcg tgcatttacc cgccagagca
      661 tgagcacact cgggaatgcc tgggtcgatc tgctacgcat cacgttatgg gtgctagtcc
      721 ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg tgcgctgcaa aactttctgc
      781 cctatcaggc ggtgaatacc gttgaaggag cacaacagct gttacccatg gggccagtag
      841 cttctcagga agcgatcaaa atgctcggaa ctaacggcgg tggctttttt aatgccaact
      901 cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt cgtgcaaatg ctggcgattt
      961 tcttgatccc aacagcgctg tgctttgcct ttggtgaagt ggcgggcgat cgtcgccagg
     1021 ggcgcatgtt gctgtgggcg atgtcggtga tttttgtcat ctgcgtaggc gtagtgatgt
     1081 gggcagaagt tcagggtaat cctcatctgc tggcgctcgg cgcggacagc agcatcaata
     1141 tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag cctgtttgcg gtcgtgacta
     1201 cggcggcttc ctgcggcgcg gtgattgcga tgcatgattc gtttaccgct cttggtggca
     1261 tggtgccgat gtggttgatg caaattggtg aagtggtatt cggcggcgtc ggttccggcc
     1321 tttacggcat gatgctgttt gtcctgctgg cggtgtttat tgccgggctg atgattggtc
     1381 gtacaccgga atatctgggt aaaaaaatcg acgtacgcga gatgaaactg actgcactgg
     1441 cgattctggt caccccgacg ctggtgctga tgggcgcggc gctggcaatg atgaccgacg
     1501 ccggacgtag cgccatgctc aaccctggcc cgcatggttt tagcgaagtg ctgtacgccg
     1561 tgtcatccgc cgctaacaac aacggcagcg cctttgccgg attaagcgcc aactctccat
     1621 tctggaactg tttactggcg ttgtgcatgt ttgtcgggcg cttcggggtg attatcccgg
     1681 tgatggcaat tgccggttcg ctggtgagta aaaagagcca acccgccagc tccggcacat
     1741 tgccaacgca cggcccgctg tttgttggcc tgttaatcgg caccgtgttg ctggttggcg
     1801 cactgacctt tatccctgcc ctggcgcttg gtccggtggc ggaatatctc tcctgatgat
     1861 attgagtgag cactgaatat gagtcgtaaa caactggcgc tattcgaacc aacacttgtc
     1921 gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc aggcgcaatg gcgcaatccg
     1981 gtgatgttta tcgtctggat cggcagtctg ctgaccacct gtattagcat cgcgatggca
     2041 agcggtgtga tgcctggtaa tgcgctgttt agcgcggcaa ttagcggctg gctgtgggtc
     2101 accgtactgt tcgctaattt cgccgaggcg ttggcagaag gccgcagtaa agcgcaggcc
     2161 aacagtctga aaggggtgaa aaaaaccgct tttgctcgca agctgcgcga accgaaatat
     2221 ggcgctgtgg cagacaaagt tcctgccgat caactgcgta aaggcgatat cgtactggta
     2281 gaagctggcg atattatccc ctgcgatggt gaagtcattg aaggcggcgc atcggtcgat
     2341 gaaagcgcca tcaccgggga atcggctccg gtgatccgtg aatccggcgg tgattttgcc
     2401 tccgtcactg gcggcacgcg tattctttct gactggctgg tgattgagtg cagcgttaac
     2461 cccggcgaga catttctgga tcggatgatc gcgatggtgg aaggtgcgca gcgacgtaaa
     2521 acgccgaacg agattgccct gaccattctc ctgattgccc tgactatcgt ctttttactg
     2581 gcaaccgcca cgctgtggcc gttttccgcg tggggcggta atgcagtcag cgtaacggta
     2641 ctggtggcac tgctggtctg tctgatccca accactattg gcggcctgtt gtcagcgatt
     2701 ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtta ttgccaccag cgggcgtgca
     2761 gttgaagcgg cgggcgacgt ggacgtcctg ctgctggata aaaccggcac catcacactc
     2821 ggtaaccgtc aggcgtcgga gtttatccct gcgcagggcg tggatgaaaa aacgctggct
     2881 gacgccgcac aactggcttc gctggctgat gaaacgccgg aaggccgcag tattgtgatc
     2941 ctcgccaagc agcgttttaa cctgcgcgag cgcgatgtgc agtcgctcca tgccaccttt
     3001 gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca tcgacaaccg catgatccgt
     3061 aaaggttctg tcgatgccat tcgccgccat gttgaggcta acggcggtca cttccctgcc
     3121 gatgttgatc aaaaagtcga tcaagttgcg cgtcagggag ccacgccgct ggtggtggtg
     3181 gaaggttctc gtgtgctggg cgttattgcg ctgaaagata tcgtcaaagg cggtattaaa
     3241 gagcgcttcg cccagctgcg taaaatgggc attaaaacgg tgatgattac cggcgataac
     3301 cgtctgactg ccgccgcgat tgccgcggaa gcgggcgtcg atgattttct cgccgaagca
     3361 acaccggagg ccaagctggc gttgattcgt cagtatcagg cagaaggccg tttagtggcg
     3421 atgaccggcg atggcaccaa cgatgctccg gcgctggcgc aggcggatgt cgcggtggcg
     3481 atgaactccg gcacccaggc ggcgaaagaa gcaggcaata tggtggatct cgactccaac
     3541 ccgaccaagt tgatcgaggt ggtgcacatt ggcaaacaga tgctgatgac ccgtggctcg
     3601 ttgaccacct tcagcattgc caacgatgtg gcgaaatact tcgccattat tccagcggcg
     3661 ttcgcggcaa cgtatccgca gttaaatgcg ctgaacatca tgcgcctgca ttcgcccgac
     3721 tccgccattc tcagtgcggt cattttcaac gccttgatta tcgtcttttt gattcccctg
     3781 gcgttaaaag gcgtgagtta taaaccgctt accgcttctg ccatgttgcg ccgtaactta
     3841 tggatttacg gtctgggtgg gctgctggtg ccgtttattg gtatcaaagt cattgattta
     3901 ctgctaaccg tttgcggtct ggtgtgaggt ttaccatgag tggattacgt ccggcattat
     3961 cgacatttct ctttctgtta ttgattactg gcggcgttta cccgctgctg accaccgcac
     4021 tggggcaatg gtggtttccc tggcaggcca acggttcatt gattcgtgaa ggtgatacgg
     4081 tgcgcggttc ggcattaatc gggcaaaatt ttaccggcaa cggctatttt catggtcgcc
     4141 cgtcggcaac agcagaaatg ccctataatc cacaggcttc tggcgggagc aatctggcag
     4201 tcagtaaccc ggtgctggat aaacagatag ccgcacgcgt tgccgcatta cgggccgcta
     4261 acccggacgc cagcacgagc gttccggttg aactggtgac agcgtcagca agcgggctgg
     4321 acaataatat taccccgcaa gcggcggcct ggcagatccc acgcgtggcg aaagcgcgta
     4381 atctcagcgt tgaacagctc acgcaactga tcgcaaaata cagccaacaa ccgctggtga
     4441 actatatcgg ccagccggtc gtcaacgttg tagaactcaa tctggcgctg gataagcttg
     4501 atgaataacg aacccttacg tcccgacccc gatcgtctgc tggaacaaac tgccgcgccg
     4561 catcggggga agctgaaagt tttcttcggt gcctgcgcag gcgtcggaaa gacctgggcg
     4621 atgctggcag aagcccagcg actgcgggcg caagggctgg atattgtggt tggcgtggta
     4681 gaaacccacg ggcggaaaga taccgccgca atgctggaag gtctggctgt tctgccgctg
     4741 aaacgccagg cgtaccgtgg gcggcatatc agcgagtttg atctcgatgc cgccctcgcc
     4801 cgccgtccgg cactgatctt aatggacgaa ctggcgcaca gtaatgcgcc aggttctcgt
     4861 catcctaaac gctggcagga catcgaagag ctgctggaag caggcattga tgttttcact
     4921 accgtcaacg ttcagcatct ggaaagcctg aacgatgtgg tcagtggcgt aaccggaatt
     4981 caggttcggg aaaccgtgcc cgatcctttt ttcgatgccg ccgacgacgt agtactggtg
     5041 gacttgcccc cggacgatct gcgccaacga ctgaaagaag gcaaagtcta tattgccggg
     5101 caggcagaac gcgccattga gcattttttc cgcaaaggca atctgatcgc cctgcgcgag
     5161 ctggcgctac gccgtactgc cgatcgcgta gatgagcaaa tgcgcgcgtg gcgggggcat
     5221 cctggcgaag agaaagtctg gcacacgcgc gacgcgatcc ttttatgcat cgggcataac
     5281 accggcagtg agaaactggt ccgcgcggcg gcgcggctgg cgtcgcgcct gggcagtgtc
     5341 tggcacgcgg tgtatgttga aacccctgcc ctgcatcgct taccggagaa aaaacgtcgg
     5401 gcaattctca gcgccttacg tctggcgcag gagttgggcg cggagacggc aacactttct
     5461 gatccagcgg aagaaaaagc agtagtgcgt tatgcccgtg aacataatct cggcaagatt
     5521 attctcggtc gcccggcctc gcgccgctgg tggcgtcggg aaacgtttgc tgaccggctg
     5581 gcgcgcatcg cccccgatct cgatcaggta ctggtcgcgc ttgatgaacc acccgcccgc
     5641 acgattaata acgcgccgga tagccgctct tttaaagaca agtggcgggt acagattcag
     5701 ggatgcgtgg ttgccgccgc gttatgcgcc gttatcacct taattgccat gcagtggctg
     5761 atggcgtttg atgccgccaa cctggtgatg ctgtatctgc ttggcgtggt tgtggtggca
     5821 ctattttacg gacgctggcc ttcggtggtt gcgaccgtca ttaatgtggt gagtttcgat
     5881 ctctttttta tcgccccacg cggcacgctc gccgtctctg atgtgcaata tctgctgacc
     5941 ttcgcggtga tgttaaccgt cgggctggtg atcgggaacc ttactgctgg cgtgcgttat
     6001 caggcgcggg tagcccgtta ccgcgaacaa cgcacacggc acttatatga aatgtccaaa
     6061 gctctggctg tgggccgtag ttcgcaggat atcgccgcca ccagcgaaca gtttattgcc
     6121 tccacgtttc atgcccgtag ccaggtgttg ctgcctgatg acaacggtaa attgcagcct
     6181 ttaacacatc cgcaaggaat gacgccgtgg gacgatgcca tcgcgcagtg gagttttgat
     6241 aaaggcctgc ctgcgggcgc gggcaccgac acgttacccg gtgtaccgta ccagattttg
     6301 ccgctaaaaa gcggcgagaa aacctacgga ctggtggtgg tggaaccggg gaatctgcgc
     6361 cagttgatga tcccggaaca gcagcgcctg ctggagacgt ttacgctgtt agtcgccaat
     6421 gcccttgagc gactgacact gaccgccagc gaagaacagg cgcggatggc aagcgaacgt
     6481 gaacagatcc gcaacgccct gctggcggcg ctttcgcatg atttacgcac gccgcttacg
     6541 gtgctgttcg gtcaggcaga aatcttaacg ctcgatctgg caagcgaagg atcgccccac
     6601 gcccgccagg ccagcgagat tcgtcagcat gtgctgaaca ccacccgact ggtgaataat
     6661 ctactggata tggcgcgaat tcagtccggc ggctttaatt tgaagaaaga gtggttaacg
     6721 ctggaagaag tggtcggcag cgcgctgcaa atgctggaac cgggtttatc gtcgcccatc
     6781 aatctttctc tgccagagcc gctgacctta atccacgttg acgggccact ctttgaacgg
     6841 gtgctgatta acctgctgga gaacgcggtg aaatatgcgg gggcgcaggc cgaaattggt
     6901 atcaatgccc acatcgaggg cgaaaatcta caactggatg tctgggataa cggccccggt
     6961 cttccaccag gccaggagca gacgatattt gataagtttg ctcgcgggaa taaagagtcg
     7021 gcggtaccgg gtgtagggct tgggttggca atttgtcggg cgatagtgga tgtacacggc
     7081 ggcaccatta ccgcattcaa ccgaccggaa ggtggtgcct gttttcgtgt tacacttccc
     7141 cagcaaacag cccctgaact tgaagaattt catgaggata tgtgacaaac gttctgattg
     7201 ttgaagatga acaggctatt cgtcgctttc tgcgcacggc gctggagggc gatgggatgc
     7261 gcgtctttga ggccgaaacg ctgcaacgcg gcttgctgga agcggctacc cgtaagccag
     7321 atttgattat tctcgatctc ggcctgcccg atggtgatgg gattgagttt atccgcgacc
     7381 tgcgccagtg gagcgcggtg ccggtgattg tgctttccgc acgtagcgaa gagagcgaca
     7441 aaatcgccgc gctggatgcc ggagcggatg attatctgag taagccgttt ggcattggcg
     7501 aattgcaggc ccgtctgcgc gtcgctttac gtcgccactc cgccaccgcc gcgcccgatc
     7561 cgctggtgaa attttccgat gttaccgtcg atttagccgc ccgcgttatc catcggggcg
     7621 aggaagaggt gcatctcacc ccgattgagt tccgcctgct ggcagtgttg ctcaacaacg
     7681 ccggaaaagt gctcacccag cgccagttac tcaatcaggt gtggggacca aacgcggtcg
     7741 aacacagcca ctatttgcgt atttatatgg ggcatctgcg acaaaaactg gagcaagatc
     7801 ccgcccgccc ccgccatttt attactgaaa ccggtattgg gtatc
//
LOCUS       DACADF010000190         7832 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.192, whole genome shotgun sequence.
ACCESSION   DACADF010000190 DACADF010000000
VERSION     DACADF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.192"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            40..1029
                     /gene="tdcB"
                     /locus_tag="ISB30_004286"
     CDS             40..1029
                     /gene="tdcB"
                     /locus_tag="ISB30_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAP1991854.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            1051..2382
                     /gene="tdcC"
                     /locus_tag="ISB30_004287"
     CDS             1051..2382
                     /gene="tdcC"
                     /locus_tag="ISB30_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAP1991855.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            2408..3616
                     /gene="tdcD"
                     /locus_tag="ISB30_004288"
     CDS             2408..3616
                     /gene="tdcD"
                     /locus_tag="ISB30_004288"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAP1991856.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            3650..5944
                     /gene="tdcE"
                     /locus_tag="ISB30_004289"
     CDS             3650..5944
                     /gene="tdcE"
                     /locus_tag="ISB30_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HAP1991857.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDTYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            5958..6347
                     /locus_tag="ISB30_004290"
     CDS             5958..6347
                     /locus_tag="ISB30_004290"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAP1991858.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            6419..7783
                     /gene="tdcG"
                     /locus_tag="ISB30_004291"
     CDS             6419..7783
                     /gene="tdcG"
                     /locus_tag="ISB30_004291"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAP1991859.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
BASE COUNT         1898 a         1871 c         2067 g         1996 t
ORIGIN      
        1 ggttacctac atatttaatt caggctaaga ggttttataa tgcatattac atacgatctc
       61 ccggttgcta ttgatgacat cattgaagca aaacaacgac tggctgggcg aatttataaa
      121 acaggtatgc ctcgctccaa ctattttagt gaacgttgca aaggtgaaat attcctcaaa
      181 ttcgaaaaca tgcagcgtac tggttcattt aaaattcgtg gcgcatttaa taaattaagt
      241 tcactgactg atgcggaaaa acgtaaagga gtagtggcct gctctgcggg caaccatgcg
      301 caaggggttt cgctctcctg cgcgatgctg ggtattgacg gtaaagtggt gatgccaaaa
      361 ggtgcgccga aatccaaagt cgcggcaacg tgcgactact ccgcagaagt cgttttgcat
      421 ggtgataact tcaacgacac tattgctaaa gtgagcgaaa ttgtcgaaat ggaaggccgt
      481 atttttatcc caccttacga tgatccgaaa gtgattgctg gccagggaac gattggtctg
      541 gaaattatgg aagatctcta tgatgtcgat aacgtgattg tgccaattgg cggcggcggt
      601 ttaattgctg gtattgcggt ggcaattaaa tctatcaacc cgaccatccg tgttattggc
      661 gtacagtctg aaaacgttca cggcatggcg gcttctttcc actccggaga aataactacg
      721 caccgaacta ccggcaccct ggcggatggt tgtgatgtct cccgcccggg taatttaact
      781 tacgaaatcg ttcgtgaatt agtcgatgac atcgtgctgg tcagcgaaga cgaaatcaga
      841 aacagtatga ttgccttaat tcagcgcaat aaagtcgtca ccgaaggcgc aggcgctctg
      901 gcatgtgctg cattattaag cggtaaatta gaccaatata ttcaaaacag aaaaaccgtc
      961 agtattattt ccggcggcaa tatcgatctt tctcgcgtct ctcaaatcac cggtttcgtt
     1021 gacgcttaat taattcgttg aggataggat atgagtactt cagatagcat tgtatccagc
     1081 cagacaaaac aatcgtcctg gcgtaaatca gataccacat ggacgttagg cttgtttggt
     1141 acggcaatcg gcgccggggt gctgttcttc cctatccgcg caggttttgg cggactgatc
     1201 ccgattcttc tgatgttggt attagcatac cccatcgcgt tttattgcca ccgggcactg
     1261 gcgcgtctgt gtctttctgg ctctaaccct tccggcaaca ttacggaaac ggtggaagag
     1321 cattttggta aaactggcgg cgtggttatc acgttcctgt acttcttcgc gatttgccca
     1381 ctgttgtgga tttatggcgt taccattacc aacaccttta tgacgttctg ggaaaaccag
     1441 ctcggctttg caccgctgaa tcgcggcttt gtggcgctgt tcctgttgct gctgatggct
     1501 ttcgtcatct ggtttggtaa ggatctgatg gttaaagtga tgagctatct ggtatggccg
     1561 tttatcgcca gcctggtgct gatttctctt tcgctgatcc cttactggaa ctctgcagtt
     1621 atcgaccagg ttgacctcgg ttcgctgtcg ttaactggtc atgacggtat cctgatcacc
     1681 gtctggctgg ggatttccat catggttttc tcctttaact tctcgccaat cgtctcttcc
     1741 ttcgtggttt ctaaacgtga agagtacgag aaagattttg gtcgcgactt caccgagcgt
     1801 aaatgttcac aaatcatctc tcgcgccagt atgctgatgg ttgcagtggt gatgttcttt
     1861 gcctttagct gcctgtttac gctgtctccg gcgaatatgg ctgaagcgaa agcgcagaat
     1921 attccagtgc tttcttatct ggctaaccac tttgcgtcca tgaccggtac caaaacaacg
     1981 tttgcgatta cactggaata tgccgcttcc atcatcgcac tcgtggctat tttcaaatct
     2041 ttcttcggtc actatctggg gacgctggaa gggctgaatg gtttgattct gaagttcggt
     2101 tataaaggtg acaaaaccaa agtgtcgctg ggtaaactga atactatcag catgattttc
     2161 attatgggct ccacctgggt tgttgcctac gccaacccga acatcctcga cttgattgaa
     2221 gccatgggcg caccgattat cgcatcactg ctgtgcctgt tgccgatgta tgccatccgt
     2281 aaagcgccgt ctctggcgaa ataccgtggt cgtctggata acgtgtttgt taccgtgatt
     2341 ggtctgctga ccatcctgaa catcgtatac aaactgtttt aatccgtaac tcaggatgag
     2401 aaaagagatg aatgaatttc cggttgtttt ggttattaac tgtggttcgt cttcgattaa
     2461 gttttccgta ctcaatgcca gcgactgtga agtattaatg tcaggtattg ccgacggtat
     2521 taactcggaa aatgcattct tatccgtaaa tgggggagag ccagcaccgc tggctcacca
     2581 cagctacgaa ggtgcattga aggcaattgc atttgaactg gaaaaacgga atttaaatga
     2641 cagtgtggct ttaattggcc accgcatcgc tcacggcggc agtattttta ccgagtccgc
     2701 cattattacc gatgaagtca ttgataatat ccgtcgcgtt tctccactgg cacccctgca
     2761 taattacgcc aatttaagtg gtattgaatc ggcgcagcaa ttattcccgg gcgtaactca
     2821 ggtggcggta tttgatacca gtttccacca gacgatggct ccggaagctt atttatacgg
     2881 cctgccgtgg aaatattatg aagagttagg tgtacgccgt tatggtttcc acggcacgtc
     2941 gcaccgctat gtttcccagc gcgcacattc gctgctgaat ctggcggaag atgactccgg
     3001 cctggttgtg gcgcatcttg gcaatggcgc atcaatctgc gcggttcgca acggtcagag
     3061 tgttgacact tcaatgggaa tgacgccgct ggaaggcttg atgatgggta cccgcagtgg
     3121 tgatgtcgac ttcggggcga tgtcctgggt cgccagccaa accaaccaga gcctggggga
     3181 cctggaacgc gtagtgaata aagagtcggg attattaggt atttccggcc tttcttcgga
     3241 tttacgtgtt ctggaaaaag cctggcatga aggtcacgaa cgcgcgcaac tggcaattaa
     3301 aacctttgtt caccgaattg cccgtcatat tgccggacac gctgcttcat tacatcgcct
     3361 ggatggaatt atattcaccg gcggaatagg agagaattca agtttaattc gtcgtctggt
     3421 catggaacat ttagctgtat taggcgtaga aattgataca gaaatgaata atcgctctaa
     3481 ctcttttgga gagcgaattg tttccagtga aaatgcgcgt gtcatttgcg ccgtaattcc
     3541 gactaacgaa gaaaaaatga ttgctttgga tgccattcat ttaggcaaag tgaacgcgcc
     3601 cgcagaattt gcataattta gttgaagtat tgtagagaga ttatttttca tgaaggtaga
     3661 tattgatacc agcgataagc tgtacgccga cgcatggctt ggctttaaag gtacggactg
     3721 gaaaaacgaa attaatgtcc gcgattttat tcaacataac tatacacctt atgaaggcga
     3781 tgaatctttt ctcgccgaag cgacgcctgc caccacggaa ttgtgggaaa aagtcatgga
     3841 aggcatccgt atcgaaaatg cgactcacgc gccattggat ttcgatacca atattgccac
     3901 cacaattacc gctcatgacg cgggatatat taaccagccg ctggaaaaaa tagtgggtct
     3961 gcaaacggat gcgccattaa agcgtgcgct gcatccgttc ggcggcatta atatgattag
     4021 aagttcattc cacgcgtatg gtcgtgaaat ggatagcgaa tttgaataca tctttaccga
     4081 tctgcgtaaa acccataatc agggcgtatt tgatacttac tcaccggata tgctgcgctg
     4141 ccgtaaatct ggtgtactga ccggtttacc agatggttat ggtcgtgggc gtatcatcgg
     4201 tgactatcgc cgtgtggcac tgtacggcat cagttatctg gtacgtgaac gcgaactgca
     4261 gtttgccgat ctccagtccc gtctggaaaa aggcgaagat ctcgaagcca ccattcgtct
     4321 gcgtgaggaa ctggcagaac atcgccgtgc gctgttacag attcaggaaa tggcggcgaa
     4381 atatggcttt gatatctctc gtccagcgca gaatgcacaa gaagccgtgc agtggctcta
     4441 cttcgcttac ctggcggcag tgaaatcgca aaatggcggc gcgatgtcgc tggggcgcac
     4501 ggcatcgttc ctcgatatct acattgagcg cgactttaaa gcaggcgtac tcaatgagca
     4561 gcaggcacag gaactgatcg accacttcat catgaagatc cgtatggtgc gcttcttgcg
     4621 tacgccggaa tttgattcgc tgttctccgg cgacccgatc tgggcgacgg aagtgatcgg
     4681 cgggatgggg ttggacggtc gaaccctggt gaccaaaaac tccttccgct atttacacac
     4741 cctgcacact atggggcctg caccggaacc taacctgact attctgtggt cggaagcctt
     4801 gccgattgcc ttcaaaaaat atgccgcgca ggtgtctatc gtcacatcct ctttgcagta
     4861 tgaaaacgac gatctgatgc gtactgactt caatagcgac gattacgcga ttgcctgttg
     4921 cgtcagccca atggtgattg gtaagcagat gcagttcttc ggtgcgcgcg ctaacctggc
     4981 gaaaacgcta ctgtacgcaa tcaacggcgg aatggacgag aagctgaaga ttcaggttgg
     5041 cccgaaaacg gctccgttga tggatgacgt gctggactac gacaaagtga tggatagcct
     5101 cgaccacttc atggactggc tggcggtaca gtacatcagc gcgctgaata tcattcacta
     5161 catgcacgac aagtacagct acgaagcttc gctgatggcg ctacatgatc gtgatgtcta
     5221 tcgcaccatg gcgtgcggca tcgcgggcct gtcggtggca acggactccc tgtctgccat
     5281 caaatatgct cgtgtgaaac caatccgtga tgaaaacggc ctggcggtgg atttcgagat
     5341 tgacggcgaa tatccgcagt atggcaacaa cgacgagcgc gtagacagca ttgcctgcga
     5401 tctggttgaa cgctttatga agaaaattaa agctttgcca acctatcgca atgctgtgcc
     5461 gacccaatcg attctgacta tcacctctaa cgtggtgtac ggtcagaaaa ccgggaacac
     5521 gccggacggt cgccgcgccg gaacaccgtt cgcgccgggc gctaacccga tgcacggtcg
     5581 tgatcgcaaa ggtgccgtgg cctcattgac gtcggtggcg aaactgccgt tcacctacgc
     5641 caaagatggg atctcgtaca ccttctccat cgtccctgcg gcgctgggca aagaagatcc
     5701 agtacgtaaa accaaccttg tcggcctgct ggatgggtat ttccaccacg aagcggatgt
     5761 cgaaggcggt caacacctca acgtcaacgt aatgaatcgg gaaatgctgc tggatgccat
     5821 cgagcacccg gaaaaatatc ctaacctgac aatccgtgtc tctggctacg ccgtgcgctt
     5881 caacgcactg acccgtgaac agcaacagga tgttatttca cgtaccttta cccaggcgct
     5941 ctgacgccgg aggatgtatg aaaaagatta tcgaaacgca acgtgcccca ggcgcaatcg
     6001 gcccttatgt tcagggcgtt gatttaggca gcatggtctt cacctccggg caaattccgg
     6061 tttgcccaca gaccggtgag atcccggctg atgtgcaaga tcaggcgcgt ttaagcctcg
     6121 aaaacgtcaa agcgatcgtg gttgctgccg ggctgagtgt gggcgatatc atcaagatga
     6181 ccgtgttcat caccgatctg aatgattttg ccaccatcaa tgaagtctat aagcagttct
     6241 tcgatgaaca tcaggcgacc tacccgaccc ggagctgtgt gcaggtcgcg cgtttgccga
     6301 aagatgtgaa gctggaaatt gaagccatcg cagtacgtag tgcgtaaagc ctcgtgagcg
     6361 ggacggtcgc aaggtcgttc cgctccactt cactgaacgg caatccgagg gtgtggatat
     6421 gattagtgca ttcgatattt tcaaaattgg gattggtccc tccagttcgc atactgtggg
     6481 gccaatgaat gccggaaaaa gttttattga tcggctggaa agtagcggct tattaaccgc
     6541 gacgagccat attgtggtcg atctgtacgg gtcgttgtca ctgacgggca aaggccatgc
     6601 cacggatgtc gccatcatca tgggactggc aggaaacagt ccgcaggatg ttgtcattga
     6661 tgagatccct gcatttatag agttagtgac gcgcagcggg cggttgccgg tggcttctgg
     6721 cgcgcatatt gttgattttc ctgtagcaaa gaacattatc ttccatcccg aaatgttgcc
     6781 tcgccatgag aacggaatgc ggatcactgc ctggaaggga caggaagagc tattaagcaa
     6841 aacctattac tctgtcggcg gcgggtttat tgtcgaagaa gaacacttcg gcctgtcgca
     6901 cgatgtcgaa acacccgtac cttacgattt ccactcagca ggtgaactgc tgaaaatgtg
     6961 tgattacaac ggcctgtcta tatctggcct gatgatgcac aacgagctgg cgctgcgcag
     7021 taaagcggaa attgacgcag ggtttgcccg tatctggcaa gtgatgcatg acggtattga
     7081 acgtggaatg aatactgaag gcgtgctgcc tggtccgctc aatgtgccgc gccgtgccgt
     7141 agcgctgcgt cgtcaactgg tttccagcga taacatctct aacgatccga tgaatgtcat
     7201 cgactggatc aacatgtacg cgctggcggt tagtgaagaa aacgcagctg gcgggcgcgt
     7261 ggtaacggca ccgactaacg gtgcgtgcgg cattattccg gcagtgctgg cttattacga
     7321 taagttccgt cgtccggtaa acgagcggtc aattgcccgc tattttctgg cagcgggggc
     7381 aattggcgcg ctgtataaaa tgaacgcctc catctctggc gcggaagtcg gctgtcaggg
     7441 ggagattggc gtggcctgtt caatggcagc ggcagggtta actgaactac tgggtggcag
     7501 tccggcgcag gtatgcaatg cggcggaaat cgcgatggag cataaccttg ggctgacctg
     7561 cgatccggtt gccggacagg tacaaatccc gtgcattgaa cgtaatgcca ttaatgccgt
     7621 gaaagcagta aacgccgcgc ggatggcgat gcgccgcacc tcggcaccgc gtgtttcact
     7681 cgataaagtg atcgagacga tgtatgaaac cggcaaagat atgaacgata aataccgcga
     7741 aacatcacgc ggaggactgg ctattaaagt ggtctgcggc tgacattgct gtcagctatc
     7801 atccttgggt gcacaaaatg tgcacccttt tt
//
LOCUS       DACADF010000191         7758 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.193, whole genome shotgun sequence.
ACCESSION   DACADF010000191 DACADF010000000
VERSION     DACADF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.193"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            148..390
                     /locus_tag="ISB30_004292"
     CDS             148..390
                     /locus_tag="ISB30_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991860.1"
                     /translation="MNPLAGMDMSRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLVNQSQTARTSVPGIWQGCEEDGVEYLRKLREKW"
     gene            complement(572..1501)
                     /gene="fdhE"
                     /locus_tag="ISB30_004293"
     CDS             complement(572..1501)
                     /gene="fdhE"
                     /locus_tag="ISB30_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAP1991861.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AEHNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1498..2133)
                     /gene="fdoI"
                     /locus_tag="ISB30_004294"
     CDS             complement(1498..2133)
                     /gene="fdoI"
                     /locus_tag="ISB30_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAP1991862.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2130..3032)
                     /gene="fdxH"
                     /locus_tag="ISB30_004295"
     CDS             complement(2130..3032)
                     /gene="fdxH"
                     /locus_tag="ISB30_004295"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAP1991863.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFNVPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3045..6095)
                     /gene="fdnG"
                     /locus_tag="ISB30_004296"
     CDS             complement(3045..6095)
                     /gene="fdnG"
                     /locus_tag="ISB30_004296"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5508..5510),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAP1991864.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSTWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6289..7122
                     /gene="fdhD"
                     /locus_tag="ISB30_004297"
     CDS             6289..7122
                     /gene="fdhD"
                     /locus_tag="ISB30_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAP1991865.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7210..7698
                     /locus_tag="ISB30_004298"
                     /pseudo
     CDS             7210..7698
                     /locus_tag="ISB30_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT         1784 a         2020 c         2064 g         1890 t
ORIGIN      
        1 ctggttttcg taataactgc aactgttaca tcgtatctgg aaaacgcctc gcaaaaataa
       61 aaagtgatgc gtaaatgcgc tttctttaaa gattcgacaa gggtactttt ctctccatag
      121 attcaaaatg atattggcga gatatttatg aaccctctgg caggtatgga tatgagcaga
      181 attttactcg atttatcgaa tgaggtgatt aagcaacttg atgatcttga ggtgcagcgt
      241 aatcttccgc gtgcagacct attacgggaa gcggtagatc aatacctggt aaatcaatcg
      301 caaacagcaa gaaccagtgt tcctggcatc tggcaagggt gtgaggaaga tggtgtcgaa
      361 tacctgcgta agctgcgcga gaaatggtaa tggcaaaaag atttggctat ttgatatcga
      421 tatccttatc gattttctca gcgtgaaaat tgaggccaaa caggcactgg cggcatagct
      481 tgaggaggta acgcggaata cgagagatag tgtcgtcaca ccctatgaac tgtaatccgg
      541 gcgacctgcg ccacccggaa aacgtcagag attactcccc ttcacccgga aacaggaacg
      601 ggttgatgga actacgggca tagccttctt gctccattcg cgcgtccagt accagagagg
      661 cgaggtcatc tgccacggct tcaactttcg gttctttttc ctgataaaga atcttcaggt
      721 aagtgccgca gtcatcgcag ctttcggctt taatcgcggc ctgttcgtca tccagcgacc
      781 agtaatgcag tttgccgctc tgttcacagt tgctgcattt tacgcgcact acgtgccatt
      841 cagtttcaca caggttgcag tgcaggtaac gcagaccctg agtggtgcca atttgcacca
      901 tgctggacac cggcatagag ccacataccg ggcaatattg acgttgttcg ccgtattcag
      961 cgcgggcttt gccggggatc agattagcca tctgcgccca gtagagcgac agcgcagccc
     1021 agataaacgg cgctttatcg ctgctgacgg acgagaaatc agaggcaaac agtgcgctgg
     1081 ccatatcttc cagctcctga gtcgatgcct tctccagatt ctcaatcact gccagcgccg
     1141 ggccgctcat ttcaggtttc agctcagcaa tcagcgccat cagcagcttt tgccagtgct
     1201 tatcacgcgg cagaacgtga atatccagcg ggggcttgcc ttgtgcgctg gcttctttaa
     1261 tgcgcgtggt cagatccatc tccagcggat ggtcgtacag caccacttcc tgggcgtggg
     1321 cgataagcgc agcaaagcgc aggtaatcac ccagcggatt atgttctgcc agctcgcgca
     1381 gacgctcggc gcggcggttg tataaattct tgagccgagg gaacaataac ggcggaatca
     1441 tatccgccgt acgtttctcg ctcgaaccca gctcatcttg cgggattatg cgaatactca
     1501 ttcagctttc ttttccgttg tcttgcggac ctcacggtac cagcgcgggt gatgtttctt
     1561 tgcccatgcg ctggtcaccc atccttccac catggcggta atcgtgcctt tcacccaaag
     1621 ggcggcgtag atatgcacca tgataaccac aattaacgcc actgcggcaa atgaatgcag
     1681 cattaacgcg aatcggatca ccgggattga gaaagcaggc gcaaaataag gacgccagat
     1741 aatcacgccg ctcaccagca acaggaccag gaaaataatc gccgcccaga aaacgcattt
     1801 ctgaccgaag ttataacgcc cggtgtcacc tacttcctcg ttgacgacga tcttacgaat
     1861 attcttcgcc caaaagatat catcccgatt gattaggttg tgatgccagt aacggaaaaa
     1921 catgatgatg aacgaggcaa acataaccac gccgacaaac ggatgcagaa ttcgcgccag
     1981 ctgcggtgtg cccatgattt gcatcaacca gttgaaggac gggaacaaaa agcccagccc
     2041 gctcaccgcc gccaggatga agcagaaggc ggtgatccag tggttgatac gttccggcgc
     2101 ggtgtagcgc acgatggtgt cacgtcgttt catttgcgct cctcgtcttt ctcttcgtgc
     2161 agattattct cttcctcatc cgcacggttc ggaccgacac ccacgtagtg gaagatactg
     2221 gctgcgaagg tagccgcaaa gccaacagct gcgagtggtt tccagatgcc tttccagaat
     2281 ttcacggttt cgctgatttc cgggttctcc ggcaagccat gatacagatt tggcttgtca
     2341 gcatggtgca gtacgtacat aacgtgtgta ccaccgacgc ctgccggatc gtacagaccc
     2401 gcattgtcgt aaccgcgggt tttcagctcc gccacgcgct cgctcgccag cgttttcatc
     2461 gactctttcg taccgaagtg aatcgcgccc gttgggcagg tcttcacgca ggccggttct
     2521 tgcccaacca ccacgcggtc aacgcacaac gtacatttgt agacgcggtt gtcttccggg
     2581 ttgaggcgtg gcacgttgaa cgggcaccct gcgatgcaat aaccgcagcc gatgcactgc
     2641 tcggactgga agtcgacgat accattggca tactgaataa ttgccccttc cgccgggcat
     2701 gctttcaggc agcctggatc ggaacagtgc atacagccgt ctttgcggat cagccattcc
     2761 agtttgtcgt tctgctccac ttccgagaat cgcatcaccg tccacgattt ggcacttaaa
     2821 tcattggggt tgtcatacac cccaatgtta ttgccgacgg tatcgcgaat gtcgttccac
     2881 tctgaacacg ccacctgaca ggctttacag ccgatacagg tggtaacgtc gatgagtttc
     2941 gccacttctt cctggaagtc ccgcgcctga ggcgcggggg tcagaccgtt agtcgcggaa
     3001 cgacgaatga tatcttgcga ttgataagcc atatgtcgtc tccgttacac cttttccaca
     3061 ttcacaagga aggacttaaa ctccggcgtc tgcgtgttcg catcaccgac gaatggcgtc
     3121 aacgtattgg caataaagcc ttttttcgca acaccttcat agccccagtg aatcggaata
     3181 ccgatggtat cgatatcttt gccgtttgct ttcagcgtgc gaatacgttt ggtcactacc
     3241 gctttggctt tgatatagcc acggttggag gagactttca cagtatcgcc ctgggcaatg
     3301 ccaagtttat tcgccagcga ctccccgatt tccacaaact gctctggttg caaaatcgcg
     3361 ttcaacagcg cgtgtttggt ccagtagtgg aagtgctcgg tcagacgata ggtggttccg
     3421 acatacggga acttatcggc tttccccaat gcttcggcat cgtctttaaa gatacgcgcc
     3481 gccggattcg agataacgtt tggatgcagc gggttagttc ccagcggcgt ttcaaacggc
     3541 tcgtagtgtt ccgggaacgg accttccgcc atcttatcga gggcaaacag acgccccatg
     3601 ccttcctgct gcatgataaa cggcccgacg ccactgcccg gaggcgctgc gctgtagtcc
     3661 ggaatatccc agccggtcca cttagtgccg tcccatttca gcaactgacg cttcggatcc
     3721 cacgggttac cctgcggatc tgcggaggcg cggttataca gaatgcggcg gttaagcggc
     3781 catgcccatg cccagcccag cgtgttaccg aggccagacg gatcggcgtt atcacgacgc
     3841 gccatctggt tgccttctgg cgtccagcta ccggcgaaaa tccagcagcc acaggaggtt
     3901 gtaccgtcat cgcgcagttg agcgaacgag ctaagttgtt ggcctttctt gacaataacc
     3961 gcaccggttg ccggatcggt aatatcggcc agcgccttac cgttgctctc catcgccact
     4021 tcttccgact tcggctcatg cgggatggcg tagttccagg tcatgttcag cacctggtcc
     4081 gggttcgcgc caccctgttc ggcatacatc ttgcgcaagc gcaggaagat accggagagg
     4141 atctcgccat cagtcagcgc aatccccggg gcgtccgcac ctttccagtg ccactgcaac
     4201 cagcgaccag agttaacgat tgaaccgttc tcttccgcga agcaggtcga tggcagacgg
     4261 aacacttcgg tctggatctt cgacgagtca acttcgttca gttcaccgtg gttctgccag
     4321 aagttagagg tttcagtgtt cagcgggtcg atagtcacga ggaatttcag cttcgacaga
     4381 cagccgatca ctttgttttt gttcgggaat gaggcaacag ggttaaagcc ctggcagata
     4441 tagccattga ccttgccctc tttcatcatc tcgaagtatt gcaggacgtc gtagccttta
     4501 tcccacttcg gcaaccagtc aaagccccag ctattttccg ccgtcgcttt atcaccaaag
     4561 aaggccttca tcatagagac gaagaatttc gggtagttgc cccagtagtt aacctggcct
     4621 tccagcagtg gtttcggcgt gttggcggta aggtaagttt gcagatcggt ctgcttctcg
     4681 cttggcagcg tcatgtaacc tggcagactc tgcgacagca gccccaggtc cgtcagcccc
     4741 tgaatattgg agtgaccgcg cagggcgtta acgccgccgc cagccatccc catattgccg
     4801 agcagcaact ggatcatcgc catcgtacgg atgttctgcg caccgatgga gtgttgcgtc
     4861 cagccgaggg cgtaaaggaa cgaggcagtt ttatcgtgag cactggtctc tgcgatgtat
     4921 tcgcagactt tcaggaatgc gtcttttggc gtaccgcaga tgttttcaac cacatctggc
     4981 gtgtagcggg aaacgtgctg tttcagcaag ttccacacgc aacgtggatg ttgcagggta
     5041 gtatcgcgtt tggcgaagcc gttttcgtcc agttcgtaag tccaggtgga cttatcgtac
     5101 ttgcgttttt ccgcgtcgta gccggtgaac aggccatctt caaagccgta atcctcacgc
     5161 acgatcaggc tagcgttggt ataggcttcg gtgtattcgc ggttgaattt ttcattgttc
     5221 agcaggtaca gcaatacgcc tgacaggaaa gcaatgtcag taccggaacg gataggggca
     5281 tagtagtcag ccaccgccgc tgtacgcgta aagcgaggat cgatcacaat cagcttcgcg
     5341 ccgttgtgaa ttttggcttc catcgcccag cggaacccga ccgggtgagc ttcagcggcg
     5401 ttaccgccca tcaccacgac gaggttggca ttcttgatgt cgacccagtg gttggtcatc
     5461 gcaccgcgac caaatgttgg agcaagactt gctaccgttg gtccgtgtca gacacgcgcc
     5521 tggttgtcga ccgcgagcat acccagcgcg cgggagaatt tttgcgttaa atagccggtt
     5581 tcgttgctcg acgcggaagc acacagcatc ccggtggaga gccagcggtt aacagtcacg
     5641 ccttcggcgt tttgcgcaat gtagtttgca tcgcggtctt ctttcatcag tttggcgatg
     5701 cgatcaaacg cctcttccca actgatttgc tgccatttat cagaacctgg cgcacggtat
     5761 tccggaaact tcagacggct ttcggagtgg atgaaatcca ccaggccagc gcctttcgga
     5821 caaagtgctc cgcggttgac cgggtgatcc gggtcacctt cgatatggaa gatagatgct
     5881 ttggcgtttt ttgctccgtc accgaggctg tacatcaaca gcccacagcc tacggaacaa
     5941 taggtgcagg tattacgggt ttcgcgggtg cgcagcagtt tatactgccg ggtttccgcg
     6001 agtgctacgc tgggtgcaaa acccagtgcc gctgccgtgg tgcctgccat accgccagcg
     6061 cagatcttaa agaactgcct tctgctgacc tgcatggatt gctccttgtt tcgacattgt
     6121 cacgtcccat ttacattcgc ttgctgcgtg tgcagggagt gggagttatt tttctttgcg
     6181 gaaggggccg caaaggtcca gaattggctc aatttccctc catccaggaa gggtttgtaa
     6241 cagaatacca taatgttggt gtgtgtgttc ttatctggtt aatagaaagt gaaaaaaaca
     6301 cagcaaaaag aaatcgagaa tgtgacaaat atcacaggtg ttcgtcaaat tgagttatgg
     6361 cggcgtgatg atttacaaca cccacggtta gatgaggtgg cggaagaagt tcccgttgcg
     6421 ctggtctaca acggcatttc gcacgtggtg atgatggcgt cgcccaaaga ccttgagtac
     6481 tttgcgctcg gtttttcgct ttccgaagga attatcgaaa gcccgcgcga tatcttcggc
     6541 atggatgtcg ttccttcctg taatggcctg gaagtacaaa ttgagctttc cagccgccgc
     6601 tttatggggt tgaaggaacg tcgccgggcg ctggcgggac gtacgggctg tggcgtatgc
     6661 ggtgtagaac agcttaacga tatcggtaaa ccggtgcagc cgctgccgtt cacccagacg
     6721 tttgatctca acaaactgga tgatgcatta cgtcatctca atgatttcca gccagtgggg
     6781 caactgactg gttgtactca cgccgctgca tggatgttgc catctggcga actggtcggc
     6841 gggcatgaag acgtgggtcg ccatgtggcg ctggacaaac tgttaggccg ccgatcacaa
     6901 gaaggggaaa gctggcagca aggtgcggtg ctggtttcca gccgtgccag ttatgaaatg
     6961 gtgcaaaagt cggcgatgtg cggcgtagag attttgtttg cggtgtctgc cgcgaccaca
     7021 cttgctgtag aagtggctga gcgctgtaat ctgacgctgg tgggcttttg taaaccgggt
     7081 cgggcgacgg tttataccca tccgcagcgt ttgagcaatt aattaaaata agtcctgcga
     7141 tatatattaa attacaggac ttattcattt cgtgaatttt gttatttttt tataaacaaa
     7201 acgcgtgtag tggcgttaat gcctgcgctg caacctattg atggcgtggc ggtgagttat
     7261 attgatgctg ccgtagcgtt gggtaatacc attaacgaaa tggataaata ttacacccag
     7321 gaaaattata aagacgatgc gtttgcgaaa gggaaaacat tccactacta ctctctggca
     7381 gtcatgattt cagcgaaaca aattaataac ctgatatcgc aagataagtt tgatgcagaa
     7441 gcggcaatga agaaagtgtc tgaactggaa acgctggtgg cgcaagccaa agaagcggat
     7501 aaaagcggca tgaatttctc gtttattaat tcggcaggcc agtatcaact cgaggctaaa
     7561 aaatacgttc gccgcatcag agataaagtc ccgtactctg actgggataa agagcaactt
     7621 caggacgcaa actcaagctg gatggccgaa gactcatttc cacgagcgtt atgcgattac
     7681 aacgaaatgg tcgatgaaat atttcagtta attgtaatcg cggggcgggt ttgtgatgag
     7741 cacggatatg tcacgaag
//
LOCUS       DACADF010000192         7728 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.194, whole genome shotgun sequence.
ACCESSION   DACADF010000192 DACADF010000000
VERSION     DACADF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.194"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            15..821
                     /locus_tag="ISB30_004299"
     CDS             15..821
                     /locus_tag="ISB30_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP1991866.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYESHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            831..1238
                     /locus_tag="ISB30_004300"
     CDS             831..1238
                     /locus_tag="ISB30_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAP1991867.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            1238..1732
                     /locus_tag="ISB30_004301"
     CDS             1238..1732
                     /locus_tag="ISB30_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAP1991868.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            1798..2595
                     /locus_tag="ISB30_004302"
     CDS             1798..2595
                     /locus_tag="ISB30_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP1991869.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            2606..3430
                     /locus_tag="ISB30_004303"
     CDS             2606..3430
                     /locus_tag="ISB30_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP1991870.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNVVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3482..4711
                     /locus_tag="ISB30_004304"
     CDS             3482..4711
                     /locus_tag="ISB30_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAP1991871.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5093..5782
                     /gene="pepE"
                     /locus_tag="ISB30_004305"
     CDS             5093..5782
                     /gene="pepE"
                     /locus_tag="ISB30_004305"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAP1991872.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIIDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(5872..7503)
                     /locus_tag="ISB30_004306"
     CDS             complement(5872..7503)
                     /locus_tag="ISB30_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAP1991873.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
BASE COUNT         1835 a         1980 c         2066 g         1847 t
ORIGIN      
        1 atcaggagtc tgttatgcaa acgtggttaa atttgcagga taaaatcatt attgtcaccg
       61 gtggcgcatc cggcattggt ctggcgattg tggatgaatt attagcacaa ggcgcgaatg
      121 tacagatggt cgatattcac ggtggcgatg gtcagtatga aagccataaa ggttatcagt
      181 tctggccgac cgatatttcc agcgccaaag aggtaaatca tacggtagca gaaattatcc
      241 agcgttttgg tcgcatcgac ggtctggtta acaacgccgg ggtcaatttc ccgcgtctgc
      301 tggtcgatga gaaagcgcct gccgggcagt atgaactcaa cgaagctgca ttcgaaaaaa
      361 tggtcaatat caaccagaaa ggcgtttttc tgatgtcgca ggcggtggcg cgacagatgg
      421 tcaaacaaca tgatggcgtg attgtgaatg tttcctcaga aagtgggctg gaaggctcag
      481 aaggccaaag ctgttacgcc gcgaccaaag ccgcgctcaa tagcttcacg cgctcctggt
      541 cgaaagagct gggtaagcac ggtatccgtg tggtcggtat cgcgccgggg attctggaaa
      601 aaacagggct gcgcacgccg gaatatgaag aggcgctggc gtggacgcgc aatatcaccg
      661 tcgagcagct gcgtgaaggc tataccaaaa acgccattcc tattgggcgc gccggaagat
      721 tagcagaagt ggctgatttt gtttgttatc tgctgtctga acgcgccagc tatatcaccg
      781 gagtaaccac taacattgcg ggcggcaaaa cgcgcggcta aggaggcttt atggtcaatg
      841 ccattttttg tgcgcatggc aaattagctt gcgccatgct ggaatcggtt cagatggttt
      901 acggcgatgc cagggtcgag gcagttgaat ttgtgcccgg cgagaacgcc ggagacatcg
      961 tagcaaagct ggaaaagtta gtaagcattc acaatcaaga tgagtggctg attgccgtgg
     1021 atttacagtg cggcagcccg tggaatgcgg cagcgatgct tgcgatgcgc aatcctcgtc
     1081 tgcgggtaat cagcggcctt tctctgccgc tagcgctcga actggttgat aaccaggaca
     1141 gcatgaatgt tgatgaactg tgtgaacacc tgacgcaaat cgcaaagcaa acctgcgtcg
     1201 tctggagaca ggtggcaaca gcggaggagg atttctgatg aacattacgc tcgcccgcat
     1261 tgatgaccgc ctgatccacg gccaggtcac taccgtctgg tctaaagtgg ctaacgccca
     1321 gcgcattatt atctgcaatg acgaagttta taacgatgaa gttcgccgga cattattgcg
     1381 tcaggctgcg ccaccgggaa tgaaggttaa cgtggtcaat attgaaaaag ccgtcgcggt
     1441 gtatcacaac ccgcaatatc aggacgaaac cgttttttat cttttcactc gcccacagga
     1501 tgccttagca atggttcgcc agggggtaaa aataggtacc ctgaatattg gcggcatggc
     1561 ctggcgtcct ggtaaaaaac agttaaccaa agcagtttca ttagatgacg atgatattaa
     1621 cgcatttcat gagttaaata atcttggcgt catactggat ttacgcgtcg tagcgtcaga
     1681 tccctcaatc aatattatcg acaaaataaa tgaacagtta atcgcgaatt aaataatagc
     1741 gcctgtgtac atatgcttta acaggcaggg atgacgacac ttaaaaggtg acaaatcatg
     1801 gaaataagta ctctacaaat cattgctata tttctttttt cctgtattgc cggaatgggc
     1861 agcgtgctgg atgaatttca gactcatcgt ccgttaattg cctgtacggt gattggttta
     1921 attctcggtg atttaaaaac cggaattatg ctcggtggta cgctggaatt gatagctctc
     1981 ggctggatga acgtcggcgc ggcgcaatct ccggattctg cgctcgccag cataatctcc
     2041 gccattctgg ttatcgttgg tcagcaaagc atcgccaccg gaatcgccat cgcgttgcct
     2101 gtggctgcgg caggccaggt gctgacggtg tttgcccgta ccatcaccgt agtgttccag
     2161 cacgcggcgg ataaagcagc ggaagaagca cggtttcgca ctctcgatat tctgcatgtc
     2221 tccgcgcttg gcgtgcaggc actgcgcgtt gctattccgg cactgattgt ctcgctgttc
     2281 gtcagcgctg atatggtgag caatatgctg agcgccattc ccgaatttgt gacccgtgga
     2341 ctgcagattg ctggcggttt tatcgtggtg gtcggttacg ccatggtgct tcgcatgatg
     2401 ggcgtgaaat atttgatgcc tttctttttc ctcggtttcc tcgcaggtgg ctacctcgat
     2461 ctcagtctgc tggcgttcgg tggcgtcggc gtgatcatgg ccctgctcta catccagtta
     2521 aatccacagt ggcgtaaagc tgaaccacat ccccagacca ccactatcac cgcccttgac
     2581 caacttgatg attaacggag ccatcatgga acagagaaaa attacacgca gcgatctggt
     2641 gagcatgttt ctgcgctcca acctgcaaca ggcatccttt aactttgaac gtattcacgg
     2701 gctgggtttt tgctacgaca tgatccccgc catcaaacga ctttacccat taaaagagga
     2761 tcaggttgcg gcgctcaggc gacacctggt gttcttcaat accacgccag ccgtatgtgg
     2821 cccggtcatc ggcgtcactg ccgccatgga agaggcgcga gccaacggcg cggaaattga
     2881 tgacggtacc attaacggca tcaaagtcgg tctgatggga ccattggcag gagttggcga
     2941 tccactggtc tggggaacgc tgcgcccgat taccgccgcg ctcggcgcat ctctggcact
     3001 ttcgggcaac attctcggcc ccctgctgtt cttctttatt ttcaacgtgg tgcgcctggc
     3061 gatgaagtgg tatggcctac agctcggctt tcgcaaaggg gtgaatatcg tcagcgatat
     3121 gggcggtaat ttgctgcaaa aactcaccga aggcgcgtcg attctcgggc tgtttgtgat
     3181 gggcgtgctg gtgaccaaat ggacgtcaat caacgtaccg ttggtggttt cacaaacgcc
     3241 tgccgccgat ggtgccaccg tcaccataac cgtgcagaac atcctcgacc aactttgccc
     3301 tggtttgctg gcgctcggtc tgacgctgct gatggttcgt ctgctcaaca aaaaaattaa
     3361 cccggtatgg ctgattttcg ccctgtttgg tttagggatt atcggtaatg cgctgggctt
     3421 cctgtcctga ttcttcgccc cggcgcaacc gtcggggcca tcgttcaaca cgaggtggtt
     3481 tatgaaaaca acagctctgc gtctttatgg taaacgtgat ttacgcctgg aaacctttga
     3541 ccttcctgaa atgcaggagg atgaaatcct cgcaacggtg gtcactgaca gcctgtgcct
     3601 ctcttcctgg aaagaggcca atctgggtga aaatcataaa aaagtacccg acgatgtggc
     3661 gaccaatccc atcatcatcg gccacgaatt ttgcggcgat attctggccg tgggtaaaaa
     3721 gtggcagcac aaattccagc cgggtcagcg ttatgtgatt caggccaacc tgcaactccc
     3781 cgaccgcccg gattgccccg gctactcctt cccgtgggta ggcggcgaag cgacgcatgt
     3841 ggttattccc aacgaggtca tggaacaaga ttgcctgctg gcatacgacg gcgaaaccta
     3901 ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt ggcgcgttca acgccaacta
     3961 tcatcttcag gaaggtagtt ataaccacac gatggggatt cgcccgcaag ggcgcacgct
     4021 gatcctcggc ggcaccggac caatgggact gttggcgatt gattatgcgc tacatggacc
     4081 cgttaaccct tcgctgctcg tcattaccga taccgacaac gacaaattga gttatgcgcg
     4141 caagcactat ccatcagaac cgcaaacact gattcattat ctcaatgccg ccgatgcagc
     4201 atttgatacg ttaatggcac tgagtggcgg tcacggcttc gatgatattt tcgtctttgt
     4261 gcctaatgaa ggactggtga ctctcgcctc ttccttgctg gcgacagatg gttgtctgaa
     4321 tttcttcgcc ggaccgcagg ataagcactt cagcgcgcca attaatttct acgatgtgca
     4381 ttatgcattt acccactacg tgggcacgtc aggcggcaat accgacgaca tgcgcgcagc
     4441 ggtcaaatta attgaagaga aaaaagtgca ggccgcaaaa gtggtaacac atattcttgg
     4501 gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc gtcggcggcg gcaaaaagct
     4561 ggtgtatacc gggaaatacc tgccgctgac gtcactcacg cagattcagg atcaagcact
     4621 ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt gaggcggagc aatacctgct
     4681 cgcccatgca gaggcaattt cccatgatta atcgcgctac ttaactctgc atgtcgctgg
     4741 ctggtcgccg cggcaacttc gcgacctgat gtgcggctga ttgctgaagc ggcacaatgt
     4801 gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg ctcaaattgg tggcaaagtg
     4861 cacattgttt atgccggatg cggaatgaag ccttattcgg cctacataat cttgaaaatt
     4921 caatatattg cagttatctg gtaggccagg taagcgtagc gcatcaggca atcctgtgtt
     4981 tgtcatcagt ctcagcgcct gtgcaatatg tgacatcgtg agccatgtca gtaagtggtt
     5041 aaattcgcag tggtagcatt ccttccgtgt tttcttacta accggagaag taatggaact
     5101 gcttttattg agtaactcga cgctgccggg taaagcctgg ctggaacacg cgctgccgct
     5161 gattgctgaa cagttgcagg gtcgccgttc tgcggtattt attcctttcg ctggcgtaac
     5221 gcagacctgg gatgattaca cagcgaaaac ggctgcggtt ctcgctccgc tgggtgtttc
     5281 tgtcaccggt attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg cggaaattgt
     5341 gattgtcggc ggcggtaata ctttccagtt gctgaaacag tgccgcgagc gcgggctgct
     5401 ggcacccatt actgacgtgg tgaaacgtgg cgcgctgtat attggctgga gcgcaggcgc
     5461 taaccttgct tgcccaacca ttcgtaccac caacgatatg ccgattatcg atccgcaagg
     5521 tttcgatgcg ctaaatctgt tcccgctaca aatcaacccg cacttcacca acgcgctgcc
     5581 ggaaggccat aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc
     5641 agaactgacg attattggtc tgccggaagg taactggatc acagtaagta aaggtcacgc
     5701 tacgctgggt ggcccgaaca ccacttatgt atttaaggct ggtgaagaag cggtgccgct
     5761 ggaagcgggt caccgttttt aagtgaattg ccggatgcat gatccggcat cttgttcata
     5821 gtgcctgatg caacgcttta cgcgtcttat caggccttca gggtcacggt attaatactc
     5881 gtcccgtcct tcatcttcat ccggctgttc cagcacactg taagccaccg aacaaaacag
     5941 cgagttcaga cgctgcatat ctcccagtaa gcctaaatgt agcgaactgg tttcaatact
     6001 ctgcacgttt tgctggtgca ggcgatcgac gtgggcgtgg gaatagcggc gattaagaat
     6061 gcgaaaacga tgtttgctgc ggcgcaaacg acgggcgctg gtaacatcgc cagagaagaa
     6121 aaccgacatt gccagtttta aattactgag caattgctca tagagcgcat ccagttcctt
     6181 caagccatca agcgaaaatg ctcgccttgc tgccagcgat ttgtcggcaa tttcgctgcc
     6241 catgcgctcg acgatatcgg aggcctgttc aaggttgagc gacatttcga tgatctccgc
     6301 ccagcggcgc gactcttctt ctgccagttc ctcttttggc atccgcgcca gatacagctt
     6361 aatggcggta tagagaacgt tgatgtcatc agccagctta cgcagctctt tctcctgccg
     6421 tggctcgccg tgcatcactt tattcagccc ttccatcatc tgttccatgg cgtcgccaat
     6481 gcgcagggtt tcgcgggcag cgttcgccag agcaagcgtg ggcgtatcca gcgcgctgac
     6541 atccagatgt ttaggccgta gctgggtatc cagttccggt tcatcgcgaa taatcgtttt
     6601 gcaaaaccgt gccatcgggt caacgaatgg caacatgacc aggcagcgta caaggttgta
     6661 gaagacgtgg aaatagatca ccaattccgc ttttggcaat ggcaacttcc ccattgtctc
     6721 tgccagcaaa tgaacaaacg gcaggataat caggctaccc accagcttaa acagcaggct
     6781 acccagcgcg acacggcggg ctgcggcatt ggcggcactg ttgttgagca tcgccagcag
     6841 gccggaaccg aggtttgcac caatcaccag acaaagcgcc actgggaaag agataatgcc
     6901 tgcggcggtc agagtcgcgg tcagcagtac cgcggcaagg ctggagtagc tgataatggc
     6961 gaacatcgca ccaatcagcg catccagcaa aatgtcgcca gtcagcgagg caaagatcac
     7021 ctgaacgccg tttgcctgag tgatcggcgt tacggcctgc acaatcaact ccagcgccag
     7081 taaaatcagc ccaagaccaa taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg
     7141 tccgaggaaa aaaatcacgc cgataaaaat aagtaacggt gacagccagg ataaatcgaa
     7201 ggtgagaata cgcgccatca gcgccgtccc gacatctgca cccagcacaa tcaccagcgc
     7261 aggcgcgaga gcaaccagat cctgagcgac gaacgaggtc accagcatgg tggtggcatt
     7321 actgctctgc accagtgcgg taacgccgat ccccgcgcaa aaggcgagcg gctttttttc
     7381 gacgctccgg ctaaggacag tacgcagacg cgcgccgaag acgcgcatta cgcctgttcg
     7441 aacaatatgg gtcccccaga ccagcagggc gacggcagaa agcaggtgaa gcaaagttag
     7501 cacggaatca ggttctcctt tttgttatgc attttcctgt taagattttt ctaatcacgt
     7561 tgtttttatc caggcatttc tcttgtatgt actttcagta taagagattt atacaagaaa
     7621 gcatttaaaa aggcatttat ataaaaatcc acctgtatct gttgctattt ctgtcacact
     7681 ttcccctgtc gtttacacca gataatcgtt gtcacttttg tgacctct
//
LOCUS       DACADF010000193         7696 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.195, whole genome shotgun sequence.
ACCESSION   DACADF010000193 DACADF010000000
VERSION     DACADF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.195"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(67..945)
                     /gene="glxR"
                     /locus_tag="ISB30_004307"
     CDS             complement(67..945)
                     /gene="glxR"
                     /locus_tag="ISB30_004307"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAP1991874.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1045..1821)
                     /gene="hyi"
                     /locus_tag="ISB30_004308"
     CDS             complement(1045..1821)
                     /gene="hyi"
                     /locus_tag="ISB30_004308"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAP1991875.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(1834..3615)
                     /gene="gcl"
                     /locus_tag="ISB30_004309"
     CDS             complement(1834..3615)
                     /gene="gcl"
                     /locus_tag="ISB30_004309"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAP1991876.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3705..4520)
                     /gene="allR"
                     /locus_tag="ISB30_004310"
     CDS             complement(3705..4520)
                     /gene="allR"
                     /locus_tag="ISB30_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAP1991877.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDNQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHL"
     gene            complement(4598..5080)
                     /gene="allA"
                     /locus_tag="ISB30_004311"
     CDS             complement(4598..5080)
                     /gene="allA"
                     /locus_tag="ISB30_004311"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAP1991878.1"
                     /translation="MKLQVLPLSQKAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5310..6236
                     /gene="allS"
                     /locus_tag="ISB30_004312"
     CDS             5310..6236
                     /gene="allS"
                     /locus_tag="ISB30_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAP1991879.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMLDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6305..7411
                     /gene="mnmH"
                     /locus_tag="ISB30_004313"
     CDS             6305..7411
                     /gene="mnmH"
                     /locus_tag="ISB30_004313"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAP1991880.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPHGILCCARGGQ
                     RSHIVQSWLHDAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENMLAAEMLKTDAHQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTYACGY"
     gene            complement(7463..>7696)
                     /locus_tag="ISB30_004314"
     CDS             complement(7463..>7696)
                     /locus_tag="ISB30_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HAP1991881.1"
                     /translation="YLRYLLIGLAPEGKIGVWLEKPDKPNIRLTDKQILIETVSGEKM
                     EMCNGRSAYKHGYSYPESTKNFIKDKKYPYGNW"
BASE COUNT         1943 a         2031 c         1828 g         1894 t
ORIGIN      
        1 ctgctttctt tttttaatat tgctggccga ttcattttct gatatcggcc atttttattg
       61 cgggtatcag gccagtttat ggttagccat taattccagc gcctgcacta atgcagagtg
      121 atccaactgg ctgccaccgt ttgccgcaca ggtattaaac aattcctggc aggtcgcggt
      181 gtttggcagg ttcagcgcca gtgctttcgc actttgcagt gccaggttga gatctttctg
      241 gtgcagcgca attttgaaac ctggattaaa ggtgcgttta atcatgcgct caccgtgaac
      301 ttccagaata cgtgaggaag caaagccgcc catcagcgcc tggcgcacac gtaccgggtc
      361 cgcaccggct tttgaagcaa acaacaaggc ttcagaaacc gcttcaatat tgagcgccac
      421 gataatctga tttgccactt tgcaggtttg accgtcgccg ttaccgccca cgagagtgat
      481 atttttaccg agcagttcaa acagcggttt tacacgttca aatactgctt catcaccgcc
      541 aaccataatc gataacgtcc cttcacgcgc gccgatttca ccgccggaga ctggcgcatc
      601 gagataatcg ccgcccagtt cattcacctg acgagcgaaa cgtttagttt caatcgggga
      661 aatggagctc atatcaacaa tggttttgcc cttcagcgag gctttggtac aaccattttc
      721 accaaacaga acgtcttcaa cctgtggcgt atccggcacc ataataaaaa tgatgtccga
      781 tgcatctgtt acctggcgcg cagtttcaac actaacggcg cccagtgaca gtaattcatc
      841 agcaaccgga ccaatggttg tgacatgcag ttgatgaccg gcacgcgcca gattaatggc
      901 catcggtgta cccataatgc ctaagccaat aaatcccagt ttcataatta acctctttta
      961 aatttcgctt ttcctgaatt cagacaacac gatcccctgc caacttagcg gcctaaaaaa
     1021 gcattgtctg aatagcgtta cgttttaacg gtacgggtcc atccagcgta aaccggcttc
     1081 cgtggtggtt tggggtttat attcacagcc aacccaaccg ttgtagtcag aattttcaat
     1141 taccgtaaag agatattcgt aattaatttc tccggttccc ggttcgccgc gatgcggatt
     1201 atcagcaatt tgcaggtgac caattttatc ggcccactga gtcatggtat tggttaattc
     1261 accttccatc cgctgcatgt gataaatgtc atactgaatt ttcaaattgc agcaaccaac
     1321 atcgtcaatc agtttcagtg cctgccgggt tccggtgaga tgaaaaccag gaatatcaaa
     1381 atggttaata ggttcaatca gtaataaaat atcttctttc atcagcatat tcgcagcata
     1441 acgcaggttt tctacaagcg ttgtgtgaat ctgttcactg ctgaaaccag tcggtgtttt
     1501 accgaccaga cagttaattt ttttattacc cagcgcacga gcataacgaa tcgctgctgc
     1561 tacgccatcc cgaaactctt cttcacggcc aggaatacag gcaatacccc gctcgcccgc
     1621 cgcccagtca cctgccggta aattgtgcag cgtatgttca agtttattac tcgccagcac
     1681 ctgttttaat tcttcaatgt cgtagtcata agggaacata aattcaacgc cgcgaaaacc
     1741 acactgcgca gctttctcaa aacgggcgag aaaatcatat tcaccaaata acatcgataa
     1801 attagcagag aaacgtaaca ttatttatct cccttattca tagtgcatga aacaggtttc
     1861 agtcggtgca tccgctgcgt tatcggcgat atcttcaaat tccatgacgt tatccagttc
     1921 actgcccatc gaaatattag tcacacgctc gagaataact tccacgacta ccggtacccg
     1981 atattgcgcc attaagattt tcgcctgttc aaaggctggt gcaatatctt ccggtttgaa
     2041 aacgcgaatt gctttacaac ctaaaccttc cgctactttt acgtggtcga cgccataacc
     2101 gttcacttcg ctggagttaa tattttcgaa cgcgagctgt acgcagtaat ccatatcaaa
     2161 ggcacgttgc gactggcgaa tcagacccag ataggcgtta ttgaccagta catggatgta
     2221 cggaatgttg aactgtgcgc caacggctaa ttcttcaatc aggaactgga agtcgaagtc
     2281 accggaaatc gccaccacat tgcgctctgg atcagcggca caaaccccca gcgccgccgg
     2341 aatcgtccag cccagcgggc ctgcctgccc acagttgatc cagtggcggt ctttaaagac
     2401 atgcagcatt tgtgccgcag cgatttgtga caggccaatg gtggtgacat aacaaacatc
     2461 gcggccaaag gctttgttca tctcttcata cacgcgctgc ggtttcaccg gcacgttgtc
     2521 gaagtgggtt ttgcgcagca aagtgcgttt gcgctgctgg cagtcggcga cccactcttt
     2581 acgacacggc agacgaccgg ctttttgcat ctcctgcgcc acttcaacca gcagcgtcag
     2641 cgccgcttta gcatcagaga caatgccaag atccggacac agcacgcggc caatttgcgt
     2701 cggctcaata tcgatatgaa cgattttgcg cccttcggtg tatttctcta ccgaaccggt
     2761 atgacggtta gcaaaacggt taccgatacc aaacaccatg tcggacgcca gcaacgttgc
     2821 attaccgtaa cgatgcgcgg tttgcaggcc caccatcccg gccatcagtt catgatcgtc
     2881 cgggatacag ccccagccca tcagcgttgg gatcaccgga atgctggtca gttcagcaaa
     2941 ctgttgtaac agtacagctg cgtcagcatt aattaccccg cctccggcaa caatcaccgg
     3001 acgttcggcc tggattaaca tttctacagc tttttcgatc tgcatacggc tggcagcagg
     3061 tttgtagacc ggcagcggtt cgtacatatc aggatcaaac tcgatttccg caacctgtac
     3121 gtcgaacggt aaatccacca gtaccggacc cggtcgacca gaacgcatca ggtgaaacgc
     3181 ctgttgcagc acacgaggca ccagcgccgc ttcacgaact gtaaccgcca ttttgctgac
     3241 cggtttagca attgcttcaa tatcaacggc ctggaaatct tctttatgca gacgggcgcg
     3301 cggagcctgg ccggtaatac acagaatagg aatggaatca gcagatgcgg aatagagcgc
     3361 ggtgatcatg tccgtgcccg caggaccgga agtccccaga catacgccga tattccctgc
     3421 cgttgcgcgg gtataacctt ccgccatgtg cgaagcacct tccacatgac gcgccagaat
     3481 gtgacgaata ccgccgtgct tacgcatcgc tgagtagaac ggattgattg cagctcccgg
     3541 aacgccgaag gcggtggtga taccttcttt ctccagcaca tacattgccg cgtcaacggc
     3601 tctcattttt gccattttat tcctacctct attaattgga aaaattttcc aactttcaaa
     3661 ttttgtcaaa aaaacccgct ccgcagagcg ggatacgaca gacactatag atgtgctttc
     3721 agtcccaacg ccgtgctgat atcgcgggcg gtgtctctga ccagctcacc ctgactgaca
     3781 aaacgatctt ctgtcagtct tgatgaaggc ccggagatgg agatagcggc aacaacacta
     3841 cctacatcat cgtaaattgc tgaagctatg caattcaggc cgacaacatg ctcttcttta
     3901 tctacggtat agcccagttc acgcgcttgt tccaggtcct tcagcaaggt gggcatatcc
     3961 acaagtgtcg ttggcgtaaa ctgctgcaaa ccggtttgca gaatgatgct catcaactct
     4021 tcttccgcca gcggataaag cagcgccttg cccgctccgg aagcatgcag cggcagacga
     4081 ctgcccagcg gcgcacacat cctgaccatc gatttacact ctaactgacc aattaatacc
     4141 gcttcattgc cgttacggat cgcgacattg accgtttcgc cggaaagtaa catcaggcgg
     4201 cgcataaacg gcccggcgac ggagaggaca tcgcggttat ggatgtacgc tgcaccgacg
     4261 ttaaagacac ctaatcctat atgccaccag cctaactgat tgtcctgata gacaaaatcc
     4321 gctgcctgta aaaccttcag caagcgaaag gtcgtggaga gcggcaaatc cagattgaga
     4381 gagatatcgc taaccgacga acttccccca cttttttcca gatattgcag aatcgcaatt
     4441 ccccgctcta acgcctgtgc gcccttctgt gctacaggct ccgcctgtcc tggcctgccg
     4501 cgccgtctaa cttccgtcat acctacgtta actccgacct acaaatgagc ataaacaatg
     4561 aacaattcgt ttatcttatg caagtcggtt gcaggcgtca cgcaaaacag agttcctgtt
     4621 caggaatact ttcaacatca cagttgtcac tgccgccgcg atcgatggtc agaaaatcgg
     4681 tgacgcgctg ccaggcgaaa agtgggtgat gccagacgtt acgatggtaa ttcactccct
     4741 gttcgccatt ggtaataaac gcccgcaggg ttgacaggtc tggtttgtcg tcacctaacg
     4801 ccaccaccac cacaaacact tcacctttca tcgggataaa cgcctgtgta cccagcggat
     4861 gacgttcgag ttcgtgaatg gtcagcggca gattcgccgg ttgcgcgcgg ttaatgctga
     4921 taagcgtacg gtcttgctca agaatctcaa ccagcgccaa atcgtggtaa cgctccacca
     4981 gtccattgtt aatatggaaa aaatcccgtt tctgcgtttc gattacgtcg ccataagcac
     5041 taaaggcttt ctgacttaac ggtaatacct gaagtttcat ctttttgtcc ttgtaacata
     5101 attccgttca accaccgccg accggtcagc ttggtttcaa taacaggcaa tctaaaacct
     5161 taagagaaca acatcaacac aaaagttgga atatttttcc aaaactagat ttagtccaca
     5221 aattgaacag ggttatcatg taaaaatact gataaatcag aggataagaa aatcacgcaa
     5281 cttgcggatt taaaagggga tacctgaaga tgttcgatcc agaaaccttg cggactttca
     5341 ttgcggttgc tgaaacaggt agtttttcaa aagcggcaga acgattatgt aaaaccacgg
     5401 cgacgatcag ttatcgcatt aaacttctgg aagagaatac cggagtggcg ctgtttttcc
     5461 gcacgactcg cagcgtgacg ttgacggcgg ctggcgagca tctactttgt caggccagag
     5521 actggctgag ctggctggaa agtatgccca gcgagttaca acaggtgaat gatggcgtgg
     5581 aacgccaggt gaatatcgtc atcaacaacc tgctctataa cccccaggcc gttgccaggc
     5641 tgctggcgtg gctaaatgaa cgttacccgt ttacccaatt tcacatctcc cgacaaatct
     5701 atatgggcgt ctgggactcg ctattgtacg aaggtttttc actggctatc ggcgtcacgg
     5761 gaaccgaggc gctggcaaat acctttagtc ttgatccctt aggatcggtg caatggcgct
     5821 ttgtcatggc ggcggatcat ccgctggcga acgttgaaga gccgctaaca gaagcacagt
     5881 tgcggcgctt tccggcggtc aatattgaag acagcgcccg caccttaacc aaacgcgtcg
     5941 cctggcgatt gccggggcaa aaagagatta ttgttcccga tatggaaacg aaaatcgccg
     6001 cccatctggc gggcgttggc attggttttt tgccaaaatc actttgccag tcaatgctcg
     6061 ataatcaaca actggtcagc cgggtaatcc caacgatgcg ccctccttcg ccattgagtc
     6121 tggcatggcg caaatttggc agcggcaaag cggtagaaga tattgtgacc ttgtttaccc
     6181 agcgcaggcc ggaaatcagc ggatttttag aaattttcgg caacccacgc agttaaaaat
     6241 caagtttcct tgcgcgttgg cgcgtaaaat agcgcgcaag ctgatttgag gttcactttt
     6301 tactatgcaa gagagacaca cggaacagga ctatcgtgcc ctgctgattg ctgatacgcc
     6361 cattattgat gttcgcgccc ccatcgagtt tgagcaaggc gcaatgcccg ccgctatcaa
     6421 cctgccgtta atgaataacg atgaacgcgc cgccgttggc atctgctata aacagcaagg
     6481 gtcagacgcg gcgctggcac tggggcataa actggtggcg ggtgaaattc gtcagcagcg
     6541 catggacgcc tggcgggcag cgtgcctgca aaatccgcac ggtattctct gctgcgcccg
     6601 tggcggtcag cgctcacata ttgtgcaaag ctggttgcat gacgcgggga ttgattatcc
     6661 gctggtggaa ggcggttata aggcactgcg ccagaccgcg attcaggcga ctattgaact
     6721 ggcacaaaaa ccgatagtgc tgattggcgg ttgtaccggc agcggtaaaa cgctgttagt
     6781 gcagcaacag ccgaacggtg ttgatctgga agggttggcg cgtcatcgcg gttcggcgtt
     6841 tggtcgcacc ttacaaccac aacttagcca ggcgagtttt gaaaacatgc tggctgccga
     6901 aatgctaaaa accgacgccc atcaggattt gcgcctgtgg gtgctggaag atgaaagccg
     6961 gatgatcggt tcgaatcacc tgccggaatg tctgcgcgaa cgaatgactc aggcgacgat
     7021 tgcggtggta gaagatccgt ttgagatccg tcttgaacgc ctgaacgaag aatatttctt
     7081 gcgtatgcat catgatttta cccacgctta tggcgacgaa cagggctggc aggagtattg
     7141 cgaatacctg catcacgggc tttcggcgat taaacgtcgg ctgggactac aacgctataa
     7201 cgaactggct gcaaggctgg atgcggcgct gacaacgcaa ctcaccaccg gcagcaccga
     7261 cggtcatctg gcctggctgg tgccgttact tgaagaatat tacgacccga tgtatcgcta
     7321 tcagcttgag aaaaaagcgg aaaaagttgt ctttcgcggt gagtgggcgg aagtggcgga
     7381 atgggttaag acttatgcct gtggttatta acccacaaaa atgcttttcc gttcctcttt
     7441 aataaattgt gcaactggtt aactaccagt tgccataggg atatttttta tctttgataa
     7501 agttttttgt actttccgga taagaatacc catgtttata ggcacttcgg ccgttacaca
     7561 tttccatttt ttcacctgaa accgtttcta tcagaatctg cttatccgtc aggcgaatat
     7621 tgggcttgtc aggtttctcc agccacactc ctattttccc ttccggagcc agcccgatta
     7681 acagataccg cagata
//
LOCUS       DACADF010000194         7627 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.196, whole genome shotgun sequence.
ACCESSION   DACADF010000194 DACADF010000000
VERSION     DACADF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.196"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            104..412
                     /locus_tag="ISB30_004315"
     CDS             104..412
                     /locus_tag="ISB30_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991882.1"
                     /translation="MNDVQNRRILALLNMLYYALGNRTTCDICHKYFILVLSVMNNLT
                     INYGSQTSPELTGISYEDANDLITELILFIEDVNADNDMEYPAHLNKIQALLNFAVAN
                     "
     gene            complement(469..1059)
                     /gene="cobO"
                     /locus_tag="ISB30_004316"
     CDS             complement(469..1059)
                     /gene="cobO"
                     /locus_tag="ISB30_004316"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAP1991883.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEDVIQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(1056..1814)
                     /locus_tag="ISB30_004317"
     CDS             complement(1056..1814)
                     /locus_tag="ISB30_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAP1991884.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2034..3083
                     /gene="sohB"
                     /locus_tag="ISB30_004318"
     CDS             2034..3083
                     /gene="sohB"
                     /locus_tag="ISB30_004318"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAP1991885.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVVTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3119..3370)
                     /locus_tag="ISB30_004319"
     CDS             complement(3119..3370)
                     /locus_tag="ISB30_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAP1991886.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            3750..6347
                     /gene="topA"
                     /locus_tag="ISB30_004320"
     CDS             3750..6347
                     /gene="topA"
                     /locus_tag="ISB30_004320"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP1991887.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            6557..7531
                     /gene="cysB"
                     /locus_tag="ISB30_004321"
     CDS             6557..7531
                     /gene="cysB"
                     /locus_tag="ISB30_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAP1991888.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         1972 a         1776 c         1974 g         1905 t
ORIGIN      
        1 tagaaatgag atccttcgtt ctcaagttat tagtggtcta caggcgtcag aggagctggc
       61 aaaacttctt attgccagcg gagttatgtt ataggaggtc attatgaacg atgtgcaaaa
      121 tcgacgaata ctggcattgc tcaatatgct gtactatgcc ttaggaaacc gaacaacctg
      181 cgatatatgt cacaaatatt ttattctggt tttgtcagta atgaataatc tgacaattaa
      241 ttatggctcg caaacgtcac cagaactgac gggtattagt tatgaagatg ctaatgattt
      301 gatcactgaa ttaatactgt ttattgaaga cgtcaatgcg gataatgata tggaatatcc
      361 cgcacacttg aataaaattc aagctcttct taattttgcc gtggctaatt aagccattca
      421 gtatctaaaa ttagcacctc aagaggcgaa cttttgaggt gctttcactt agtaatcaat
      481 ccctatctgc gcttttacac cggcatcaaa cgcatgtttg atggggcgca gctcacttac
      541 cgtatccgcc agttcaagaa tatcccgatg acagcctcga cccgtgataa tcaccgtctg
      601 ttgatgtgga cgttcattta acgcctgaat cacgtcttcc agtggcaaat agtcatacgc
      661 taccatatat gtcagttcat cgagcaaaac catatccagt gaggaatcag ccagcatccg
      721 ctttgcatgt tgccagactt cgcggcaggc ggcggtatcg gactctcggt tttgtgtatc
      781 ccaggtaaag cccgttgcca ttacctgaaa ctcaacgccg tgtggctcca gcagatttcg
      841 ttcaccatta ggccaggtgc ctttaataaa ctgcacgacg cccacttttt ttccgtgacc
      901 aactgcgcgt gttgccgtac caaatgccgc ggtggttttg ccttttccat tgccggtaaa
      961 gacgatgata ataccgcgtt catcctgggc ctgggccaca cgagcatcta ctttttcttt
     1021 cactcgctgc tgacgctgtt ggtagcgttc atcactcatt gggaaattcc tggtttacgg
     1081 cccggttggg cgtcaaaggt catgccggtt ttacggcggc tgtcatcgcc catcagccag
     1141 aggtagagcg gcatgatatc agcgggggtt ttaagtttct gtggatcttc ggtcgggaag
     1201 gcactggccc gcattgcggt gcgcgtaccg cctgggttaa tgcagttgac acgcaggcgc
     1261 tgctgatatt catcggccag tacctgcatc atcccttcgg tggcaaattt cgacgctgca
     1321 tatgcgcccc agtttgcacg cccctgacgt ccaacgcttg atgaggtaaa gaccagtgaa
     1381 ccggcgtcgg atttgagtaa taaaggaagc agtgcctggg tgagcataaa ggtggcatta
     1441 acgttgacct gcatgacgtc ctgccagact tgcggatctt gttcgctcat tgggcaaaca
     1501 tcgccgagca atccggcatt atgcaaaaca ccatccagac gcggataatt aacggcaatg
     1561 cgctgtgcca gttgttggca atcttcggac gtgcaggtta gcaaatcgag aataaaccac
     1621 tgtggctgac gtccagtttc ttcgtttatg tggctggcta cctgacgtaa tttttcttca
     1681 ttacggccca aaagaatcac tgtcgcacca tagcgtgcat acgtcatcgc tgcttcacga
     1741 ccaattccat cgctggctcc cgtcaccaga ataatgcgat cattgagtaa atcttgtttt
     1801 ggctggtaat gcatggctac tcctcaacga cgttgtctgt cgcgctgcca gtgtacttta
     1861 tgactttggg gctttatgcc tgaaacagaa gggcatttca atcagcctgg ggcgataaca
     1921 ccgcaaaatg gatactttgt catactttcg ctgcaataac atctctgcga gacggctaaa
     1981 catgcctgtt gtaaactgtg agccaaagcg ttgtttaacc aaggtgggga ctcgtggaat
     2041 tgttgtctga atatggtttg tttttggcga aaatcgttac cgttgtgcta gcgattgcgg
     2101 cgattgccgc cattattgtc aatgttgctc aacgtaataa acgccagcgt ggcgagttac
     2161 gagtcaacaa tctcagcgaa cagtataagg agatgaaaga agaactggcc gcggcgctga
     2221 tggacactca tcagcaaaaa cagtggcaca aagcgcagaa gaaaaagcac aagcaagaag
     2281 cgaaagccgc aaaagcgaaa gctaaactgg gcgaggtggt aactgacagt aaaccccgcg
     2341 tctgggtgct ggattttaaa ggcagcatgg acgcccatga agtgaactct ctacgtgaag
     2401 agataacggc ggtactcgca gcattcaaac cgcaggatca ggttgtgctc cgtctggaaa
     2461 gcccaggtgg catggtgcat ggttacgggt tggcggcttc gcagttgcag cgtctgcgtg
     2521 ataaaaacat tcctttaact gttacggtag acaaagtcgc tgccagcggc ggttacatga
     2581 tggcctgtgt ggcggacaaa attgtttccg caccgtttgc tattgtgggt tccattgggg
     2641 tggtggcgca aatgcccaac tttaaccgct tcctgaaaag caaagatatt gatatcgaac
     2701 tgcacaccgc cgggcagtat aagcgcacgc tgacgttgct gggtgaaaat accgaagaag
     2761 ggcgggagaa attccgcgaa gagttgaacg aaacgcatca gttatttaaa gattttgtga
     2821 agcgtatgcg tccgtctctg gatattgaac aggtggcaac gggtgaacac tggtacggac
     2881 aacaggcagt agagaaaggc ctggttgatg aaatcaacac cagtgatgaa gttattctta
     2941 gcctgatgga aggccgggaa gtggtcaatg tacgctatat gcagcgtaaa cgactcattg
     3001 accgattcac cggcagcgcg gcagagagcg ccgatcgatt gttgttacgc tggtggcagc
     3061 gtgggcaaaa gccgttgatg taaaagacaa acgcgaggat aagacctcgc gttttgcttt
     3121 aatcaaccag gtgatatttt tctgaaagca catgggccag gtgtttgaac atattaaaca
     3181 ccgcggtgct tttggcagtt ggtaatcctt gttcatctaa aaagtagtcg ccggtaaata
     3241 ccagcacgcc attacgctgc gtgacgccgg tggcttcaat ccctgcgagc gtatcctcat
     3301 gctcacgaat gattttgttg gcctctttca acagcgtttc gcggtcgatg ggttgagtct
     3361 ctttgttcat tatttactcc ttaaacaagg acattagtct acgccaggca tggcttgcag
     3421 acaaatatac cacgctggtg gcaagagcgc cttactggca actttggatt ttgcatgcta
     3481 ataaagttgc gtatcggatt ttatcaggta cagtgtgacg ctttcgtcaa tctggcaata
     3541 gatttgcttg acattcgacc aaaattccgt cgtgctatag cgcctgtagg ccaagacctg
     3601 ttaactcagt cacctgaatt ttcgtgagca gagtcacgac aaggggttga tatccgcaga
     3661 gagcgagtcc atatcggtaa ctcgttgcca gtggaaggtt tatcaacgtg cgacgcattc
     3721 ctggaagaat caaattaggt aaggtgaata tgggtaaagc tcttgtcatc gttgagtccc
     3781 cggcaaaagc caaaacgatc aacaagtatc tgggtagtga ctacgtggtg aaatccagcg
     3841 tcggtcacat ccgcgatttg ccgaccagtg gctcaactgc caaaaagagt gccgactcta
     3901 cctccaccaa gacggctaaa aagcctaaaa aggatgaacg tggcgctctc gtcaaccgta
     3961 tgggggttga cccgtggcac aattgggagg cgcactatga agtgttgcct ggtaaagaga
     4021 aggtcgtctc tgaactgaaa caactggctg aaaaagccga ccacatctat ctcgcaaccg
     4081 accttgaccg cgaaggggaa gccattgcat ggcacctgcg ggaagtgatt ggtggtgatg
     4141 atgcgcgcta tagccgagtg gtgtttaacg aaattactaa aaacgcgatc cgccaggcat
     4201 ttaacaaacc gggtgagctg aatattgatc gtgttaatgc ccagcaggcg cgtcgcttta
     4261 tggaccgcgt ggtggggtat atggtttcgc cgcttctatg gaaaaagatc gctcgtggtc
     4321 tgtctgccgg tcgtgtgcag tcggtggcgg tccgcctggt ggtcgagcgt gagcgtgaaa
     4381 ttaaagcgtt cgtaccggaa gagttctggg aagtcgatgc cagcacgacc acgccatctg
     4441 gtgaagcgtt ggcgttgcag gtgactcatc agaacgacaa accgttccgt ccggtcaaca
     4501 aagaacaaac tcaggctgcg gtaagtctgc tggaaaaagc gcgctacagc gtgctggaac
     4561 gtgaagacaa accgacaacc agtaaacctg gcgctccttt tattacctct acgctgcaac
     4621 aagctgccag cacccgtctt ggttttggcg tgaaaaaaac catgatgatg gcgcagcgtt
     4681 tgtatgaagc aggctatatc acttacatgc gtaccgactc caccaacctg agtcaggacg
     4741 cggtaaatat ggttcgcggt tatatcagcg ataattttgg taagaaatat ctgccggaaa
     4801 gtccgaatca gtacgccagc aaagaaaact cacaggaagc gcacgaagcg attcgtcctt
     4861 ctgacgtcaa tgtgatggcg gaatcgttga aggatatgga agcagatgcg cagaaactgt
     4921 accagttaat ctggcgtcag ttcgtagcct gccagatgac cccagcgaaa tatgactcca
     4981 cgacgctgac cgttggtgcg ggcgatttcc gcctgaaagc acgcggtcgt attttgcgct
     5041 ttgatggctg gacgaaagtg atgcctgcac tgcgtaaagg cgatgaagat cgtatcttac
     5101 cggcagtcga taaaggcgat gctctgacgc tcgttgaact gacaccagcc cagcacttta
     5161 ccaagcctcc agcccgtttc agtgaagcat cgctggtcaa agagctggaa aaacgcggta
     5221 tcggtcgtcc ttctacctat gcgtcgatca tttcgaccat tcaggatcgt ggctatgtgc
     5281 gagtagaaaa tcgtcgtttc tatgcggaaa aaatgggcga aatcgtcacc gatcgcctgg
     5341 aagagaattt ccgcgagtta atgaactacg acttcaccgc gcagatggaa aacagcctcg
     5401 accaggtggc aaatcacgaa gcagagtgga aagctgtact ggatcacttc ttctcggatt
     5461 tcacccagca gttagataaa gctgaaaaag atccggaaga gggtggtatg cgcccgaacc
     5521 agatggttct gaccagcatt gactgcccga cttgtggtcg caaaatgggg attcgcacag
     5581 cgagcaccgg ggtattcctt ggctgttctg gctatgcgct gccgccgaaa gagcgttgca
     5641 aaaccaccat taacctggtg ccggaaaacg aagtgctgaa cgtgctggaa ggtgaagacg
     5701 ctgaaaccaa tgcgttgcgc gcaaaacgtc gttgcccgaa atgtggcacg gcgatggaca
     5761 gctatctcat cgatccgaaa cgtaagttgc atgtctgtgg taataaccca acctgcgacg
     5821 gttacgagat cgaagagggt gaattccgca ttaaaggtta tgacggcccg atcgttgagt
     5881 gtgaaaaatg tggctctgaa atgcacctga aaatggggcg tttcggtaaa tacatggcct
     5941 gcaccaacga agagtgtaaa aacacacgta agattttacg taacggcgaa gtggcaccac
     6001 cgaaagaaga tccggtgcca ttaccggagc tgccgtgcga aaaatcagat gcctatttcg
     6061 tgttgcgtga cggtgctgcc ggtgtgttcc tggcggccaa taccttcccg aaatcgcgtg
     6121 aaacgcgtgc gcctctggtg gaggagcttt atcgcttccg cgaccgtctg ccggaaaaac
     6181 tgcgttatct ggccgatgcg ccacagcagg atccggaagg taataagacc atggttcgct
     6241 ttagccgtaa aaccaaacag caatatgtct cttcggaaaa agacggaaag gcgactggtt
     6301 ggtcagcatt ttatgttgat ggcaaatggg ttgaagggaa aaaataacct ttaattccgt
     6361 ctggttttta taaacaaagg gtcgcgaaag cggccctttt ttattgcata ttattttttc
     6421 ttcacaccta tacactaagg ctataaatga tatagtggtt atagttagca ccttttttat
     6481 tattaaatcg tattagtcac ccgcctggtg tggcgaaaaa acgatgttct gatggcgtct
     6541 aagtggatgg tttaacatga aattacaaca acttcgctat attgttgagg tggtcaatca
     6601 taacctgaat gtctcatcaa cggcggaagg actttacaca tcacaacccg ggatcagtaa
     6661 acaagtcaga atgctggaag acgagctggg cattcaaatt ttttcccgaa gcggcaagca
     6721 cctgacgcag gtaacgccag caggacaaga aataattcgt atcgctcgtg aagttctgtc
     6781 gaaagtcgat gccataaaat cggtcgccgg agagcacacc tggccggata aaggctcgct
     6841 gtatatcgcc accacgcata ctcaggcacg ctatgcgtta ccaaacgtta tcaaaggctt
     6901 tattgagcgt tatcctcgcg tttctttgca tatgcaccag ggctcgccga cacaaattgc
     6961 tgatgccgtc tctaaaggca atgccgattt cgctatcgcc acggaagcgc tgcatctgta
     7021 tgaagattta gtgatgctac cgtgctacca ctggaatcgg gctatagtag tcactccgga
     7081 tcacccgctg gcaggtaaaa aagccattac cattgaagaa ctggcgcaat atccgttggt
     7141 gacatatacc ttcggcttta ccggacgttc agaactggat actgccttta accgcgcagg
     7201 gttaacgccg cgtatcgtct tcacggcaac ggatgctgac gtcattaaaa cttacgtccg
     7261 gttagggctg ggggtagggg tcattgccag catggcggtg gatccggtcg ccgatcccga
     7321 ccttgtgcgt gttgatgctc acgacatctt cagccacagt acaaccaaaa ttggttttcg
     7381 ccgtagtact tttttgcgta gttatatgta tgatttcatt cagcgttttg caccgcattt
     7441 aacgcgtgat gtcgttgatg cggctgtcgc attgcgctct aatgaagaaa ttgaggtcat
     7501 gtttaaagat ataaaactgc cggaaaaata atttcttgcg ttattttcgg caccttttat
     7561 gtagcgaagg tgccggaata tattctcctt tgttacttat ttgtatccgg tgcacaatat
     7621 ttttatc
//
LOCUS       DACADF010000195         7503 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.197, whole genome shotgun sequence.
ACCESSION   DACADF010000195 DACADF010000000
VERSION     DACADF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.197"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            216..4463
                     /gene="fdeC"
                     /locus_tag="ISB30_004322"
     CDS             216..4463
                     /gene="fdeC"
                     /locus_tag="ISB30_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HAP1991889.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTANNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETESGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWIAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAELAATSDVHSLVADGVT
                     TATLTVTLFSANNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNKP
                     GTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(4588..5445)
                     /locus_tag="ISB30_004323"
     CDS             complement(4588..5445)
                     /locus_tag="ISB30_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1991890.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKLPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5693..6562
                     /locus_tag="ISB30_004324"
     CDS             5693..6562
                     /locus_tag="ISB30_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP1991891.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6722..7315)
                     /gene="rclC"
                     /locus_tag="ISB30_004325"
     CDS             complement(6722..7315)
                     /gene="rclC"
                     /locus_tag="ISB30_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HAP1991892.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(7327..>7503)
                     /locus_tag="ISB30_004326"
     CDS             complement(7327..>7503)
                     /locus_tag="ISB30_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1991893.1"
                     /translation="NADDKIILKHISVSSVSASPTVLENAIADMARKYNASSWKVTSM
                     RIDNNSTATAVLYK"
BASE COUNT         2060 a         1672 c         1920 g         1851 t
ORIGIN      
        1 tttttaccca taaataaaaa tataatcccg cgcccaacca cctgatgagt ggctacagac
       61 actggatata ttaggtggcg gtgtactttc ttacataaag gtatttcctt ttctgtggaa
      121 aaggaaaccg ggaaatcccc ggtttttctg acaagcagac gccattattt gtgtctgcct
      181 atgttcgtta attcgttcat caggaaatta tctcaatgtc acgttataaa acagacaata
      241 aacagccacg atttcgttat tcagttctgg cccgctgcgt ggcgtgggca aatatctctg
      301 ttcaggttct ttttccactc gcagtcacct ttacgccagt aatggcagca cgtgcgcagc
      361 atgcggttca gccacggttg agcatggaaa atactacggt aactgctaat aataacgtgg
      421 agaaaaatgt cgcgtcgctt gccgctaatg ccgggacatt tttaagcagt cagccagata
      481 gcgatgcgac acgtaacttt attaccggaa tggccaccgc taaagctaac caggaaattc
      541 aggagtggct cgggaaatac ggtactgcgc gcgtcaaact gaatgtcgat aaaaatttct
      601 cgctgaagga ctcttcgctg gaaatgcttt atccgattta tgatacaccg acaaatatgt
      661 tgttcactca gggggcaata catcgtaccg acgatcgtac tcagtcaaat attggttttg
      721 gctggcgtca tttttcagaa aatgactgga tggcgggggt gaatactttt atcgatcatg
      781 atttatcccg tagtcatacc cgcattggtg ttggtgcgga atactggcgt gattatttga
      841 aactgagcgc caatggttat atccgggctt ctggctggaa aaaatcgccg gatgttgagg
      901 attatcagga acgcccggcg aatggctggg atattcgtgc tgagggctat ttacctgcct
      961 ggccgcagct tggcgcaagc ctcatgtatg aacagtatta tggcgatgaa gtcgggctgt
     1021 ttggtaaaga taaacgccag aaagatccac atgcgattac cgctgaagtg aattacacgc
     1081 cagtgcctct tctgaccctg agtgccgggc ataagcaggg caagagtggt gagaatgaca
     1141 ctcgctttgg cctggaagtt aattatcgga ttggcgaacc tctggaaaaa caactcgata
     1201 cagacagcat tcgcgagcgt cgaatgctgg caggcagccg ctatgacctg gttgagcgta
     1261 ataacaatat cgttcttgag tatcgcaaat ctgaagtgat ccgtattgct ctgcctgagc
     1321 gtattgaagg caagggcggc cagacggttt ccctggggct tgtggtcagc aaagcaactc
     1381 acggtctgaa aaatgtgcaa tgggaagcgc cgtctttgct ggccgcaggc ggaaaaatta
     1441 cggggcaggg caatcagtgg caagtgacgc tcccggctta tcaggcaggc aaagacaatt
     1501 attatgcgat ttcagcgatt gcctacgata acaaaggcaa tgcctcgaaa cgtgtgcaga
     1561 cagaagtggt tattagcgga gctggtatga gcgccgatcg tacggcgtta acgcttgacg
     1621 gtcagagccg tattcaaatg cttgctaacg gtaatgagca aaagccgctg gtgctgtctc
     1681 tgcgcgacgc cgagggtcag ccagtcacgg gcatgaaaga tcagatcaag actgaactaa
     1741 ccttcaaacc ggctggaaat attgtgactc gtaccctgaa ggccactaaa tcacaggcaa
     1801 agccaacact gggtgagttc accgaaactg aatcaggggt gtatcagtct gtctttacta
     1861 ccggaacgca gtcaggtgag gcaacgatta ctgttagcgt tgatgacatg agcaaaactg
     1921 tcactgcaga actgcgggcc acgatgatgg atgtggcaaa ctccaccctg agcgctaacg
     1981 agccgtcagg tgatgtggtt gctgatggtc agcaagccta cacgctgaca ctgacagcgg
     2041 tggactccga gggtaatccg gtgacgggag aagccagccg cctgcgactt gttccgcaag
     2101 acactaatgg tgtaaccgtt ggtgccattt cggaaataaa accaggggtt tacagcgcca
     2161 cggtttcttc gacccgtgcc gggaacgttg ttgtgcgtgc cttcagcgag cagtatcagc
     2221 tgggcacgtt acaacaaacg ctgaagtttg ttgccgggcc gcttgatgca gcacattcgt
     2281 ccatcaccct gaatcctgat aaaccggtgg ttggcggtac agttacggca atctggacgg
     2341 caaaagatgc taatgacaac cctgtaactg gcctcaatcc ggatgcaccg tcattatcgg
     2401 gcgcagctgc tgctggttct acggcatcag gctggacgga taatggcgac gggacctgga
     2461 ttgcgcagat ttctctcggc actacggcgg gtgaattaga cgttatgccg aagctcaatg
     2521 ggcaggacgc ggcagcgaat gcggcaaaag taaccgtggt ggctgatgca ttatcttcaa
     2581 accagtcgaa agtctctgtc gcagaagatc acgtaaaagc cggtgaaagc acaaccgtaa
     2641 cgctggtggc gaaagatgcg catggcaacg ctatcagtgg tctttcgttg tcggcaagtt
     2701 tgacggggac cgcctctgaa ggggcgaccg tttccagttg gaccgaaaaa ggtgacggtt
     2761 cctatgttgc tacgttaact acaggcggaa agacgggcga gcttcgtgtc atgccgctct
     2821 tcaacggcca gcctgcagcc accgaagccg cgcagctgac tgttattgcc ggagagatgt
     2881 catcagcgaa ctctacgctt gttgcggaca ataaaactcc aacggttaaa acgacgacgg
     2941 aactcacctt caccgtgaag gatgcgtacg ggaatccggt caccgggctg aagccagatg
     3001 caccagtgtt tagtggtgcc gccagcacgg ggagtgagcg tccttcagca ggaaactgga
     3061 cagagaaagg taatggggtc tacgtgtcga ccttaacgct gggatctgcc gcgggtcagt
     3121 tgtctgtgat gccgcgagtg aacggccaaa atgccgttgc tcagccactg gtgctgaatg
     3181 ttgcaggtga cgcatctaag gctgagattc gtgatatgac agtgaaggtt aataaccaac
     3241 tggctaatgg acagtctgct aaccagataa ccctgaccgt cgtggacagc tatggtaacc
     3301 cgttgcaggg gcaagaagtt acgctgactt taccgcaggg tgtgaccagc aagacgggga
     3361 atacagtaac aaccaatgcg gcagggaaag tggacattga gcttatgtca acggttgcgg
     3421 gagaacacaa tatttccgct tcggtgaatg gtgctcagaa gacggtcacg gtgaaattca
     3481 acgcggatgc cagcaccggt caggcaaacc tgcaggtaga caccgccgtt caaaaagtgg
     3541 caaacggcaa agatgccttt acgctgacgg cgaacgttga ggataaaaat ggtaaccctg
     3601 ttccagggag cctggtgacc tttaatctgc cccggggtgt caagccgctt acaggcgata
     3661 atgtgtgggt gaaagccaac gatgagggga aagcggagtt gcaggtggtt tccgtgactg
     3721 ccggaaccta tgagatcacg gcgtcagcag gaaatgacca gccttcgaat gcgcagtctg
     3781 taacgtttgt ggccgataag actacggcga ccatctccag tattgaggtg attggcaacc
     3841 gtgcagtggc ggacggcaaa accaaacaga cgtataaagt tacggtgact gatgccaata
     3901 acaacctgct gaaagatagc gaagtgacgc tgactgccag cccggaaaat ttagttctga
     3961 ctcccaatgg gacggcgaca acgaatgagc aagggcaggc tattttcacc gccacgacca
     4021 ctgtcgcagc gacatataca ctcacggcga aagtggaaca ggccgacggt caggaatcga
     4081 cgaaaactgc cgaatctaaa ttcgtcgcgg atgataaaaa cgcggagctc gccgcaacat
     4141 ctgatgtgca ttctctggtg gcggatgggg taacgacagc aacactgacg gtaaccctgt
     4201 tctcggcaaa caacccggta ggaggaacca tgtgggtcga cattgaggct ccggaagggg
     4261 tgacagaggc ggattatcag ttcctgccgt cgaaaaatga ccatttcgcg agcgggaaaa
     4321 tcacgcgtac atttagtacc aacaagccag gtacatacac attcacattc aactctttga
     4381 catatggagg gtatgaaatg aaacccgtga ctgtgacaat taacgccgtt cctgcagata
     4441 ctgaaggtgc tgaggagaaa taaaaacgac gatgtaataa ataataatgg cgttgacatg
     4501 cgcagcgcca ttatttactg aatacctacc ctggcaccga ttagacggtg gcagggtagg
     4561 gtaaatctta tgtgatggag ttacaggcta aaccgggcga acggggataa aaatttcgca
     4621 aacaatgtta ttgctatcaa tttcgacgaa gttttcattg taataaaatc gctcaatatc
     4681 actgccgttc actctggcta accccctggc aggtaattcc atcagataga ggtttcgcgc
     4741 ccattcaggg tagtgctccc actcaccttc atagcggaaa gaggcataca gtcctcggtt
     4801 aattgtccac tctttcggca tctctgaatc ggtttgtttg cataccgtac cggcaaaagt
     4861 ttcaactgtg agcaaatcga ccgattttgt tgatggttcg aacgtggaga cgaaatagat
     4921 atccgtctta aaattcttca tacatctgac taaatgatga cgtcgcagtt tgacttcttc
     4981 atccggtaat ttattcgaca cttcaatatc atatttaaat gaattgccaa aaataggtgt
     5041 ctcaggaaag ttgactaatc tacactccgt cttttgctgt tgacgtatta aaaaagaagg
     5101 gaagatattt gcgagatccc aataatcacg gtggcggtat tcacggggag agcagccgaa
     5161 taacttttta aattcgcggc tgaatgactg ctgtgaatca aaatgcaaag agagtgcaat
     5221 atcaagcata gatttcgcgg agagccggac aagaatggcg gcacgacaaa gccttcgttt
     5281 gcgaatgtat tctcccaaag gcacatgcat gaaatttcgg aacagaagct ggatgttgcg
     5341 tctgctgtag ccagatttct gtgcgatatc ttcgattgaa atagggtgct caagattgca
     5401 ctcaatccac tccaggagct gctgtagaat cttctgcctg atcatcacat actccgttaa
     5461 ttctttgtca tatcgcacat tttcgttatg tcctgggccc taagtgtatc aaaaacagca
     5521 agtcccaagg caaaagcact gatagcgtct gcttcattgc cccaaaaata gagaggtttc
     5581 agtcctacat tattaatgaa ttttttgcat aagtgatatc aaaatccaca tactaatttg
     5641 aggttacgtt ttaacgtaga ctcattgttc atacctaatg gagggactga cagtggaatt
     5701 ttccgtatta agtaacaatc tgaaaatgcc gatgatggga tttggtgttt ttcaggttac
     5761 cgataaagac gtgtgcaaac agtcagtgtt gaatgctatc cgcacgggtt atcgactcat
     5821 cgacactgca gcggtatacg gtaatgaaga tgccgtcggt gaagccgttc gtgaagccat
     5881 atctgaaggt ttatgtaccc gagaagagtt atttataacg tctaaattgt gggtgcagga
     5941 tatgttgaat caggatattg cagcagcagg tattgaagca tcattaaaaa aatcggggct
     6001 agaatacttc gatctctatt tattgcacca ggccatgcgc gattatttta gtgcgtggcg
     6061 tgcactcgaa gatgcctatg aagagggcaa attaaaagcg attggggttt ccaatttcta
     6121 ccctcatgtt ctggcgaact tttgtgaaac ggtaagagtt aaaccgatgg tcaaccaggt
     6181 cgagttgcat ccctattttg cccaaccaga ggcgctggca accatgaagt attataacgt
     6241 gcagcctgaa gcatgggctc cgttaggtgg tggacgacat aaaccctttg aaaataatct
     6301 gcttcagagt attgcagatg cccatcaaaa atcgatttct caggtcattc tgcgttggaa
     6361 tattcaacgg ggagtggtcg ttattccgaa atcgacacat caacagcgta tcgaagaaaa
     6421 ttttgctatc tgggatttct cactgacaga gaaagaaatg gcgcaaatta gttcgctaga
     6481 tttgggttat gttggggaat cggtaaaaca ttttaatcct gagtttgttc gtggttgtct
     6541 tgctgtaaaa atacatgatt gatattaatc acttatttta cctgagacga caaaaatttt
     6601 ttaacagggg agtgatattg atcgtcactc tgtcatatct ccggtaatat ggcgtcaggc
     6661 ttcatcaaca gaaagggggc ctgacgcctg aaaaagtgaa caacagacag tgttcggatt
     6721 atcaatattc agtttttaac gttgaactgg attcattact gcgttgttta agaatatccc
     6781 gtgccgaatc tgccattatc atgacagcac ctgccagcat cagagtatct ttcaatacca
     6841 gacgaccagc accggataaa taagggaaac catgatgagc atcacccaat gcgggtaccc
     6901 atgcctccgg agtggtgatt aaaaatgaga gtgttaccag cggcgtggta aatgccatca
     6961 gcccacccaa taaacctaac cagcgattga caggattagc caaaaccagc agagcaataa
     7021 tcacctccac gacgccaaga ccgttggaaa aaccataggt attattagcc gtttgccatg
     7081 ctcttgcttc tggtttgtat tcgccttcgt gagtcagata ctgtttatag tcttccgggt
     7141 gttcataaaa gaacgacatt agtggactgt ttgcgacgaa tggtgtaatg ctgtctgcct
     7201 cgtaagggac aaactttaat aacccaatcc acataaaaac aatcgcaatg ctcagacgaa
     7261 tcaatgtcag gccaatttta tctccccgac ttaacaggtg caggtatctt tccattgtaa
     7321 acatccttat ttatacaata ctgctgttgc ggttgaatta ttatcaattc gcatcgatgt
     7381 aactttccag gatgaagcat tatattttct ggccatatca gcaatggcat tctcaagaac
     7441 tgtcggtgat gctgatactg atgagacgct gatatgtttc agaattattt tatcatctgc
     7501 att
//
LOCUS       DACADF010000196         7410 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.198, whole genome shotgun sequence.
ACCESSION   DACADF010000196 DACADF010000000
VERSION     DACADF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.198"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..260
                     /locus_tag="ISB30_004327"
     CDS             <1..260
                     /locus_tag="ISB30_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991894.1"
                     /translation="NIPELIVRPADGCYFSWIDCSAIGYPFDEFYSRLIHEGKVGIMA
                     GHVYGTEGEGYLRLNLACSREKLYMGLTRLVSVIKNINQGE"
     gene            263..859
                     /locus_tag="ISB30_004328"
     CDS             263..859
                     /locus_tag="ISB30_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP1991895.1"
                     /translation="MNNTDLIHLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            931..1878
                     /locus_tag="ISB30_004329"
     CDS             931..1878
                     /locus_tag="ISB30_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HAP1991896.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLSGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLVGYGNIGKILA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHAIIVLLPFSSELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGRKIKNVL"
     gene            complement(2178..3554)
                     /locus_tag="ISB30_004330"
     CDS             complement(2178..3554)
                     /locus_tag="ISB30_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein VasL"
                     /protein_id="HAP1991897.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRPAQQNDK
                     DASPPVSSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNVQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEISPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(3554..3982)
                     /gene="tssE"
                     /locus_tag="ISB30_004331"
     CDS             complement(3554..3982)
                     /gene="tssE"
                     /locus_tag="ISB30_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAP1991898.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVRERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(3986..4522)
                     /gene="tssJ"
                     /locus_tag="ISB30_004332"
     CDS             complement(3986..4522)
                     /gene="tssJ"
                     /locus_tag="ISB30_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HAP1991899.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKN
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(4503..5582)
                     /gene="tssG"
                     /locus_tag="ISB30_004333"
     CDS             complement(4503..5582)
                     /gene="tssG"
                     /locus_tag="ISB30_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HAP1991900.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQRQQNAPLGST
                     DSPAADAVRFRPHPGMGFPVSELKNVERDVDNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSPISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(5546..7306)
                     /gene="tssF"
                     /locus_tag="ISB30_004334"
     CDS             complement(5546..7306)
                     /gene="tssF"
                     /locus_tag="ISB30_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAP1991901.1"
                     /translation="MDDLTQRYFEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELTTDHQQM
                     KQSDTLKEFQVLSRPIGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPLYLSADSPVACALHRALTLGTQQFWLRLPGQGRRVLDAHFS
                     PMGFDDDDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLEQVAWPEGITGFEIDV
                     LLNENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDTDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLAAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1739 a         1901 c         1949 g         1821 t
ORIGIN      
        1 aaaatattcc agaattgatc gtaaggccag ctgatggttg ctatttttcc tggatagatt
       61 gttctgctat tggttatcct tttgatgaat tctacagtcg attaattcat gaaggcaaag
      121 tgggcattat ggcggggcat gtctatggaa cggaggggga gggctacctt cgtctgaacc
      181 tggcctgcag tcgagaaaaa ttatatatgg ggcttacgcg tcttgtgagt gtaattaaaa
      241 atattaatca gggagaataa aaatgaataa cacggatctt atccatctca ttaaacattt
      301 tatgcgtaac gaacataaag cagttgaaga agttattgat tcccctcttt cggaggtcgc
      361 aaatttaatt aaagtattac aatcttgtca gggaaaagtt gtttttattg gtgttggtaa
      421 gtccggtatt attgccagga aactcgccgc aacatttgcc agcaccggaa ctccctcgtt
      481 ttttgttcac ggtacggaag ctgtacacgg cgaccttgga atggtggcga aagacgatgt
      541 tgttattctt atttcaaaca gtggtgagac ggcggaaatc ctggcgacac tgcctagtct
      601 gaaaaaaatg ggtaattatt tgatctcttt tacccgcagt caccactcat cgcttgcgat
      661 aagttgtgat ttatctgttg aaatccccgt caaaagtgag gcggataatt taggtttagc
      721 accgtcatgc tcgtcgactg ttgttctggt tgttggtgat gcggtggcgc ttgcactctc
      781 tgaattgaaa aaatttactc gtgctgattt cggcttatat catccaggag gtgcactcgg
      841 cattaaagca aattcataat ttcaaaatat aaatacagta accggtcacg ccatcttttg
      901 gggtggcgtt ctttgaagtg agtgtccgct atgaaaaatg ttctggtcac cgtccccagc
      961 ttttcagcac gctgtgtttc cgccagcaag ttgctaagag agaataattt taatcttatt
     1021 attaagaata atgtggagca tctgcttaaa tcagaatcca ctgcattacg agaatctatt
     1081 tgcgcggtca tcgccggaaa agattgttat caggcggaca cgctatcatt gctgtctggt
     1141 gtgaaaataa tatcgagatt cgggacgggg atagacaata ttgatcttcg tgcggcacag
     1201 caatccggta ttgttgtcaa taatgcggtt gggattaatt caaatgcggt tgcggaattt
     1261 atcattggac ttatcttcgc gagcatgaga aatatccctg gcagctatca tgcgatgcaa
     1321 aatggctact ggggtgagtc gcatggctgt gaattacaag ggaaacggat tggcctggtt
     1381 ggctacggta atataggtaa aattctggcg aaaaggttat ccggttttga tgttgagctt
     1441 ttagcttttg acaaacaacc cgactatcag gttgctgaca aagctggagt tcagtttgta
     1501 tcaattgaag atatctttat gcagtcgcat gccatcattg ttcttttgcc tttttcttct
     1561 gagctggaga acttcattag tcataaatat ttgtcgatga tgcgcaatgg tgctctgatc
     1621 atcaatgccg caaggggaaa gttgctggat gagggcgctt tacttcaggt gattgaagaa
     1681 cgaaatgtgt ttgcggcgct ggatgtgttt agctctgaac cgttggcgca atttagccca
     1741 ctgctacatg ccaaaaatat cattacaacg ccgcatattg cggccgccac cgttgagtca
     1801 taccagcaaa ccgggataca cgttgcccag tcgataattg attacttcgc aggaaggaag
     1861 ataaaaaacg tgttgtgagt tcagcgaaca aagattttaa atacaaaaaa accgcaattt
     1921 gtagaacaca ttgcggtttg tcagcattct aataaattgg ttgcgggggc cacagtcaag
     1981 aggttaatcc aatcttttga aacttaaggt attcactgtt cccgaaccca agctatctac
     2041 cgaaatatac actcattatc tgatgtgaat caacgagtag atcgaatggc ctgttcctct
     2101 cttttttgta cacaaagact ccttttaaaa aatagcaagg tcacatatcc cttattggta
     2161 cataaatccc ccgatgttta ctgactttgt tccaccttcg tatccagtaa aatgacatag
     2221 cgattcagca actgatttaa ccgggtatca atctgcgtca acagcgtcgg cgaaatctct
     2281 ccggtctgtt tttgctcttc cagttgccga agcagttctt ccagcggcgg ctcctttaac
     2341 gactgccgga tgccgtacac cgccgttttt agttccgaac cggtcagata tctcccctta
     2401 cgttcatcca gtgcatccag ttgtgccgac aggcgctgta gctgactctg cgcctgtgcg
     2461 tagccattca gtgcctctgc ggacagggcg ctcagttccc gctgtttacg ccactgcacg
     2521 ttcagcgccc tgacctgagg attatccggc cacagggcat caagctggcg tagctggcga
     2581 tatccctgct ccagcggcca gcgggcaggt aatttgttta actgctccag ttgcatctga
     2641 cttgccgcaa ttgttctgtc ctgagccagc agcgccttat cctttacatc cagctttgcc
     2701 agttcgccgc tctccagcga ctgcggcaac ggcatgacag acagttgtat tcgctggatt
     2761 agcgcgtccg gctgattaag ctgcgaccag ccccataatc ctgttccgcc gagaccggcc
     2821 atcacaacca ttccggccat aaatatcggc catgtccgct gtcgggagat ttcaggggat
     2881 gacacgggtg gactggcatc cttatcgttt tgctgagcgg gtctcgttgc aaaaaccggc
     2941 tgtggacttt cttctgactt tttcggttga ttcatttccc ggcggggtaa ttcaggggga
     3001 gtgatagtgg tttccgctcc ctgaggcgcc agccgttcca gacgcaatgc ggtattgcga
     3061 aactgcccgg ccagcacatc cagtttcgtc atatgccaca gttcacactg ttgcagcgtg
     3121 gtttcgatgg cggaaagatg ctgtacacaa cggtagatct gcggcagatc ctgatactga
     3181 atatccagcg ttctcagtgc ctgctgcatt ttttctgtga cccagctgag cagtgatatt
     3241 ctggaatgta cctgtaccgg ccagaagtca gcccagcgct ggagcagtat atccagtgag
     3301 ccgagtccgt ctgccatccc tgccagcccc tgccgtctgg ttatcgccag tacgtaacag
     3361 accagcgtct gtagttccac gccattttgc cggaacagcg cgaggcagag tttttccacc
     3421 tgacgccagt ccacatccgg tcgggccggg tgagtcagtt tactcatttc agcctgcagt
     3481 tcaccggatt ccttataagt acagggatcg ccgcccatga cgaactggct gataaaattc
     3541 gcgttacttg ccatcagtac gcccgctcct gttgtttgag atgacgcaga acaattcgtc
     3601 cttccggaga aaatacgcca tcaaagcgta tccagccggt atcgggaagc tgcgcgtgga
     3661 tcaggtaatt aagatgcccc ggacggggct gggggagtaa ctccacctgt atctgactca
     3721 ggcgtggttc ataacgcagc agcgtttctt caatctgacg catcaggcca tgaatacccg
     3781 cagccatccc ctgatggatc agactgagat ccggcagacc gtaatccggc aaatgcgcca
     3841 gcgtgcctgc ccggcagttg ataatgcgtt gcatattgtc catcacggaa aggataagct
     3901 gatcccgctc tctgacctgt tccagcggta actcgccgct gaaactgaac gtcagcattt
     3961 catataagga aggtgtcgac atcggttatt tatccttgag aggcagcagt cgcaggtcgc
     4021 ttctcatcag ttcaatcgag cgcggcttgt cggcctccag ttcgtccctt ttgatcacca
     4081 gacgccagct gtcgcttttt tcatcagggt gataaaactg cccaataatg gcgacaaact
     4141 gcgtctcttt atccagcggc acattcaggg atgccgtgcc ttccggtttt acccagacgg
     4201 tgtgttttgc cagtacgttc ttctccagcg tcttctcttc ctgcataaac agggtgtcat
     4261 aatccgcctt gtcaaaatct tcgcgggttt tcagttgcca gacatgcacc atcgtggaaa
     4321 gcggcgtatc ttccgcatcc gtattcaggg cacttcggga gaagaaatcg agatgcagta
     4381 ttttgatttg cttgtagaaa acggctgagg ccacgctttt cgattcatcc accactttct
     4441 gaatcagccc gcagccgctc agaacaaccg tcagcatcgc catacagagc agcgccggac
     4501 gtttaaaacg aataggaaac atgagtcata ctctccctgt tacgaatgga aaaagcgggt
     4561 attaatccct gataacgccc cagactgacc gtcactgttc cggtcgcggg cgcggcaaag
     4621 gaggcgcgaa ggatgccggt acggctaatc tgtacacgct gttttcccag ccgggcagca
     4681 ggcaacaggc tgacgggcag cgtcagttgc agtcgggcct gacagcgcca gcccagataa
     4741 acgcgcagca gcaccagcaa atcgctgtgc agttgcccgc cgggcagcca gccacgtgct
     4801 tctgcggcat cttcggtata cagtttcagc agcaactggc tgttgacgtc ggtgccggta
     4861 cgccccagca gcgttcgcgt tttcaggctg atgggtgaat tttttgacag acccgcaggg
     4921 gccgggagaa ttacaggctg cggatcatga ggcaccaccg tcgctttcgt cagcggggca
     4981 agcagtccca ccagtgccgt aacgccttcc gccgtgcgcg tcggcaggcg catcacgctg
     5041 agcagggcca gaaaacggga taccggcgtg gcgatatggt tctcgctgcc gggaataccc
     5101 agcccgatga gtcccagcag gcaacgggaa atgtcatcca ctgcacctgc ttcaaacgac
     5161 gccgggtagt tatatttgcg ccagatgcgg taatactggg tgataaagcg atggttaaaa
     5221 atatcgagaa acgccatcac cgcgtcatgt ccgtcatgac gctgggtaat gtagtccaga
     5281 taggcggttg gcagtggcga atccacgccg tacaggccga gaaaagtggt gcgcactgtg
     5341 ggtggcgcgt cgggattgtc cacgtcacgt tcaacgttct ttaactcgct gaccggaaag
     5401 cccatccccg gatgaggacg aaagcgcacc gcatcggcgg cggggctgtc ggtactgccc
     5461 agcggcgcgt tctgctggcg ctgttccagc aactggcaaa aacggtaaaa attgatccgc
     5521 gcaatatccg gggccagccg gtcggtcagc ccggtacgcg ctggctgtga tgctctgtcc
     5581 attgcaggca ctttcctgtg ggttgcagaa ttaaggttag ttgagtgaat aagtggatat
     5641 cggcatacag tgcgaagaag cggtgcagca gttcaccaaa cagcgtgata tcaccttctc
     5701 cggcaaagcc gttactgtcc agcgtcacct gaatatccac gccacgcagc agaaagcctc
     5761 tttcaaaacg ctgtatcagg ctgtgctgaa cgtcgacaat cgccgccaga cgacggcggt
     5821 tcatctcgct ttcggtccag tcgtacagcg ccagcgtgcc gcgcagaatt tcggcgttat
     5881 ccatcatcga taaaaagtta gaaccaaggt ggctgagcac ccgccagtgg aagcggtcac
     5941 gcgccggggg atagcagggc tgcgtcgggg cgcacaggtt gcgcacccgc aggacgttct
     6001 gtgaggcatg aactggcgta tcgagcagcg tgctctgtaa agctttacgg ggaagctgcc
     6061 cgttagtacc ggtcaggctt aaggacaggg tttcatcctc cagcatccgg tcagtatcaa
     6121 acgcatcgcc gcccagaatc agccaggtgt catgcaggcc ggaagggccg cgctttaccc
     6181 gcgtgtggta atagcgttcc ggcgcatcgt ggcgcagcat cccgccccgg tgacgaaagc
     6241 tggagaatgg cacatacgcc tgactgccgg tatggcgcga agaaataatg ttgtcgacgg
     6301 aataaatctc ggtatgcccg tcctgaatgc gcatcgggcg cagcagaaat tcgttttcca
     6361 gtggcgaaag gtgtaacgga tcggcttcca gcggaaacag gttgatgacc ggcacgcagt
     6421 gcagacggat attatcgctg tcaaacggca ggtcgtgagg ccagttctcg ttcagcaaca
     6481 cgtcaatctc aaagccggtt atcccctccg gccatgcaac ctgctccagc ccgttcagcg
     6541 ccacgaacat aaacttctcg cggaaggtga aatactccag cagcaactgg tagccgctga
     6601 acgccgattc gcctttcggc cacagtcggt catcgtcatc aaaacccatc ggggaaaagt
     6661 gcgcgtccag cacacggcgt ccctgccctg gcagacgcag ccagaactgc tgcgtaccaa
     6721 gcgttaaagc ccggtgcagc gcacaggcca ccgggctgtc ggcactgagg tacagcggca
     6781 ggcgggataa atcaatctgt gaccagtcac caaccagcgg gccgcattca aagcgcagac
     6841 ggataaccga gcgtccgtcc ggttcatatt gcaatgacac gtccggcaac gccagcggat
     6901 gcagcgtgat atcccgcgtg gcgctgtaga cgcagcgggt tctgcgttcg ccgatgggac
     6961 gggatagcac ctgaaactct ttcagcgtat cactttgctt catttgttgg tgatccgtgg
     7021 tcagttcgac cacggaaagc gacggaatag tgcgcaggta gtgcggccac agcaggctga
     7081 ccagcccttc agtcagttcc ggcagatcgt cgtccagctt ttcgcgcagg cggcccatta
     7141 aaaaagcgaa gccctcgaac aggcgctcca catagggatc gcgtgcgccg ggtttatcaa
     7201 gattaagcat cgcagcgcgg tcggggtaag cctgcgcaaa ttctttcccc gcttcacgca
     7261 ggtagcgcat ttcggcctca aagtagcgtt gggtcaggtc gtccatcgga ggatttatct
     7321 ttccttttat tgaaattaat accaacgagt agtgagatta gaatttttta ttttcagaat
     7381 cctaaggaca tatagatgga tcatttattt
//
LOCUS       DACADF010000197         7399 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.199, whole genome shotgun sequence.
ACCESSION   DACADF010000197 DACADF010000000
VERSION     DACADF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.199"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            89..1423
                     /gene="cadB"
                     /locus_tag="ISB30_004335"
     CDS             89..1423
                     /gene="cadB"
                     /locus_tag="ISB30_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HAP1991902.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1503..3650
                     /gene="cadA"
                     /locus_tag="ISB30_004336"
     CDS             1503..3650
                     /gene="cadA"
                     /locus_tag="ISB30_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HAP1991903.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3709..5166
                     /gene="dtpC"
                     /locus_tag="ISB30_004337"
     CDS             3709..5166
                     /gene="dtpC"
                     /locus_tag="ISB30_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HAP1991904.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            5403..6920
                     /gene="lysS"
                     /locus_tag="ISB30_004338"
     CDS             5403..6920
                     /gene="lysS"
                     /locus_tag="ISB30_004338"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP1991905.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(7039..7212)
                     /gene="ghoT"
                     /locus_tag="ISB30_004339"
     CDS             complement(7039..7212)
                     /gene="ghoT"
                     /locus_tag="ISB30_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HAP1991906.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(7240..>7399)
                     /gene="ghoS"
                     /locus_tag="ISB30_004340"
     CDS             complement(7240..>7399)
                     /gene="ghoS"
                     /locus_tag="ISB30_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAP1991907.1"
                     /translation="PRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLSIMTVD
                     DYFRQFYK"
BASE COUNT         1834 a         1710 c         1799 g         2056 t
ORIGIN      
        1 taatcttagc gccagtttgg tctggtcagg aaatagttat acatcatgac ccggactcca
       61 aattcaaaaa tgaaattagg agaagagcat gagttctgcc aagaagatcg ggctatttgc
      121 ctgtaccggt gttgttgccg gtaatatgat ggggagcggt attgcattat tacctgcgaa
      181 cctagcaagt atcggtggta ttgctatctg gggttggatt atctctatta ttggtgcaat
      241 gtcgctggca tatgtatatg cccgactggc aacaaaaaac ccgcaacaag gtggcccaat
      301 tgcgtatgcc ggagaaattt cccctgcatt tggttttcag acaggtgttc tttattacca
      361 tgctaactgg attggtaacc tggcaattgg tattaccgct gtatcttatc tttccacctt
      421 cttcccagta ttaaatgatc ctgttccggc gggtatcgcc tgtattgcta tcgtctgggt
      481 atttaccttt gtaaatatgc tcggcggtac ctgggtaagc cgtttaacca ctattggtct
      541 ggtgctggtt cttattcctg tggtgatgac tgctattgtt ggctggcatt ggtttgatgc
      601 agcaacttat gcagctaact ggaatactgc ggataccact gatggtcatg cgatcattaa
      661 aagtattctg ctctgcctgt gggccttcgt gggtgttgaa tccgcagcag taagtactgg
      721 tatggttaaa aacccgaaac gtaccgttcc gctggcaacc atgctgggta ctggtttagc
      781 aggtattgtt tacatcgctg cgactcaggt gctttccggt atgtatccgt cttctgtaat
      841 ggcggcttcc ggtgctccgt ttgcaatcag tgcttcaact atcctcggta actgggctgc
      901 gccactggtt tctgcattca ccgcctttgc gtgtctgact tctctgggct cctggatgat
      961 gttggtaggc caggcaggtg tacgtgccgc taacgacggt aacttcccga aagtttatgg
     1021 tgaagtcgac agcaacggta ttccgaaaaa aggtctgctg ctggctgcag tgaaaatgac
     1081 tgccctgatg atcctcatca ctctgatgaa ctctgccggt ggtaaagcct ctgacctgtt
     1141 cggtgaactg accggtatcg cagtactgct gactatgctg ccgtacttct actcttgcgt
     1201 tgacctgatt cgttttgaag gcgttaacat ccgcaacttt gtcagcctga tctgctctgt
     1261 actgggttgc gtgttctgct tcatcgcgct gatgggcgca agctccttcg agctggcagg
     1321 taccttcatc gtcagcctga ttatcctgat gttctacgct cgcaaaatgc acgagcgcca
     1381 gagccactca atggataacc acacagcgtc taacgcacat taattaaaag tattttccga
     1441 ggctcctcct ttcattttgt cccatgtgtt gggaggggcc ttttttacct ggagatatga
     1501 ctatgaacgt tattgcaata ttgaatcaca tgggggttta ttttaaagaa gaacccatcc
     1561 gtgaacttca tcgcgcgctt gaacgtctga acttccagat tgtttacccg aacgaccgtg
     1621 acgacttatt aaaactgatc gaaaacaatg cgcgtctgtg cggtgttatt tttgactggg
     1681 ataaatataa tctcgagctg tgcgaagaaa ttagcaaaat gaacgagaac ctgccgttgt
     1741 acgcgttcgc taatacgtat tccactctcg atgtaagcct gaatgacctg cgtttacaga
     1801 ttagcttctt tgaatatgcg ctgggtgctg ctgatgatat tgctaacaag atcaagcaga
     1861 ccactgacga atatatcaac actattctgc ctccgctgac caaagcactg tttaaatatg
     1921 ttcgtgaagg taaatatact ttctgtactc ctggtcacat gggcggtact gcattccaga
     1981 aaagcccggt aggtagcctg ttctatgatt tctttggtcc gaacaccatg aaatctgata
     2041 tttccatttc agtatctgaa ctgggttctc tgctggatca cagtggtcca cacaaagaag
     2101 cagaacagta tatcgctcgc gtctttaacg cagaccgcag ctacatggtg accaacggta
     2161 cttccactgc gaacaaaatt gttggtatgt actctgctcc ggcaggcagc accattctga
     2221 ttgaccgtaa ctgccacaaa tcgctgaccc acctgatgat gatgagcgat gttacgccaa
     2281 tctatttccg cccgacccgt aacgcttacg gtattcttgg tggtatccca cagagtgaat
     2341 tccagcacgc taccattgct aagcgcgtga aagaaacacc aaacgcaacc tggccggtac
     2401 atgctgtaat taccaactct acctatgatg gtctgctgta caacaccgac ttcatcaaga
     2461 aaacactgga tgtgaaatcc atccactttg actccgcgtg ggtgccttac accaacttct
     2521 caccgattta cgaaggtaaa tgcggtatga gcggtggccg tgtagaaggg aaagtgattt
     2581 acgaaaccca gtctactcac aaactgctgg cggcgttctc tcaggcttcc atgatccacg
     2641 ttaaaggtga cgtaaacgaa gaaaccttta acgaagccta catgatgcac accaccacct
     2701 ctccgcacta cggtatcgtg gcgtccactg aaaccgctgc agcgatgatg aaaggcaatg
     2761 caggtaaacg tctgatcaat ggttccattg aacgtgcgat caaattccgt aaagagatca
     2821 aacgtctgag aacggaatct gatggctggt tctttgatgt atggcagccg gatcatatcg
     2881 atacgactga atgctggccg ctgcgttctg acagcacctg gcacggcttc aaaaacatcg
     2941 ataacgagca catgtatctt gacccgatca aagtcaccct gctgactccg gggatggaaa
     3001 aagacggcac catgagcgac tttggtattc cggccagcat cgtggcgaaa tacctcgacg
     3061 aacatggcat cgttgttgag aaaaccggtc cgtataacct gctgttcctg ttcagcatcg
     3121 gtatcgataa gaccaaagca ctgagcctgc tgcgtgctct gactgacttc aaacgtgcgt
     3181 tcgatctgaa cctgcgtgtg aaaaacatgc tgccgtctct gtatcgtgaa gatcctgaat
     3241 tctatgaaaa catgcgtatt caggaactgg ctcagaatat ccacaaactg attgttcacc
     3301 acaatctgcc ggatctgatg tatcgcgcat ttgaagttct gccgactatg gtaatgactc
     3361 cgtatgctgc gttccagaaa gagctgcacg gtatgaccga agaagtttac ctcgacgaaa
     3421 tggtaggtcg tattaacgcc aatatgatcc ttccgtatcc gccgggagtt cctctggtaa
     3481 tgccgggtga aatgatcacc gaagaaagcc gtccggttct ggagttcctg cagatgctgt
     3541 gtgaaatcgg cgctcactat ccgggctttg aaaccgatat tcacggtgca taccgtcagg
     3601 ctgatggccg ctataccgtt aaggtattga aagaagaaag caaaaaataa ttagctcgta
     3661 caagggaagt ggcttgccac ttcccttttt tgctcataag gagaacacat gaaaacaccc
     3721 tcacagccgc gcgcgatata ctatatcgtg gcgatccaaa tctgggagta cttcagtttt
     3781 tacggcatgc gtgccttact cattctctat ctcacccatc agcttggttt tgatgataac
     3841 catgccatca gcctgttcag cgcatatgct tctctggttt acgttacccc tattctcggc
     3901 ggctggcttg ccgaccgcct gctcggcaac cgcactgcag tgattgccgg cgcgctgtta
     3961 atgacccttg gccatgtggt gctgggcatt gatacaaatt caacctttag cctgtatctg
     4021 gcgctggcaa tcattatttg tggctacggt ttattcaaat caaacatcag ctgtttgctt
     4081 ggcgagctct acgacgagaa cgatcatcga cgtgatggcg gtttttcgct gctgtatgct
     4141 gcgggcaata tcggttctat cgccgcgccc atcgcctgcg gcctggctgc tcagtggtat
     4201 ggatggcatg ttggctttgc ccttgcggga ggcggcatgt ttatcggttt gttgattttc
     4261 ttaagtggtc atcgtcattt ccagtccacg cgtagtatgg ataaaaaagc gctcactagc
     4321 gtcaaatttg ccttaccagt atggagctgg ttagtggtga tgctctgttt agccccagta
     4381 ttttttaccc tgctgctgga gaacgactgg tccggatatt tgctggcgat cgtttgcctc
     4441 attgcagcac aaatcattgc ccgcatgatg atcaaattcc ccgaacaccg ccgtgctctt
     4501 tggcaaattg tattgttgat gtttgtcggg acgttgttct gggtactggc acaacagggc
     4561 ggcagtacca tcagcttgtt tatcgatcgc tttgtgaatc gtcaggcatt caatattgaa
     4621 gtacctacag cactattcca gtcggtgaat gctattgcgg tgatgctcgc tggggttgtt
     4681 ctggcctggc tggcgtcgcc agaaagccgc ggcaattcag cattgcgcgt ctggctgaag
     4741 tttgcctttg gcttactgct gatggcttgt ggctttatgt tgctggcatt tgatgcccga
     4801 catgcagcgg ctggcggtca agcgtcaatg ggcgtgatga tatccgggct ggcgctaatg
     4861 ggctttgctg aactctttat tgacccggtg gcgattgcgc aaatcacgcg tctgaaaatg
     4921 tctggcgtat taaccggtat ttatatgctg gcaacaggcg cggtcgccaa ctggctggca
     4981 ggcgtcgtgg cacagcagac gacagagtcg caaattagcg gtatggcaat tgccgcttac
     5041 cagcgattct tttctcagat gggagagtgg acgttggctt gtgtcgcgat catcgtggta
     5101 ttggcctttg ctacccgttt tctgtttagc acgccgacga atatgataca ggagagcaac
     5161 gattaactgt cgtaaaaaca attggttatg aataaattaa ctttcagttt atcacactag
     5221 taaatcgaga ttaataagtc atttaaacca ttttgatggt tatttattag tgatatcaac
     5281 ttgaggtaag cgttagtttc gataagataa actgagttat taatagtcga ggcagataat
     5341 acagtgtacc gatctgtctc tttatctacg ctaaattgaa agctggattt agaggaacca
     5401 aaatgtctga acgagaaaca cggggagcca atgaggctat tgattttaac gatgaactga
     5461 gaaatcgccg cgaaaaactg gcggcactac gtcagcaagg tgtggcgttt cccaatgatt
     5521 ttcgccgcga ccatacctct gaccagttgc acgaagagtt tgatgcgaag gataaccagg
     5581 aactggaatc cttaaacatt gaagtctcgg ttgctggccg aatgatgacc cgtcgtatta
     5641 tggggaaagc ctcctttgta acgttgcagg atgtcggtgg ccgtattcaa ctgtacgttg
     5701 caagagatag cctgccagaa ggtgtttata acgatcagtt taaaaaatgg gatctgggtg
     5761 acattatcgg tgcccgcggt acgctgttta agacgcaaac gggtgagctt tccattcact
     5821 gtactgagct gcgcctgctg actaaagcac tacgtccttt accagataaa ttccatggtc
     5881 tgcaggatca ggaagtccgt tatcgtcaac gttatctgga cctcatcgct aacgataaat
     5941 cccgtcaaac gtttgttgtc cgttcaaaaa ttctggccgc tatccgtcag ttcatggtcg
     6001 cgcgcggctt tatggaagta gaaaccccga tgatgcaggt aattccaggt ggggcatctg
     6061 ctcgcccgtt tattacccat cataacgctc tggatttaga tatgtacctg cgtatcgcgc
     6121 cggagctgta tctgaaacgt ctggttgtag gcggttttga acgggtattc gaaatcaacc
     6181 gtaacttccg taatgaaggt atttctgttc gccataatcc tgagttcaca atgatggaac
     6241 tctacatggc atatgcggat taccatgatt tgattgaact gacggagtca ctgttccgca
     6301 ccctggcaca agaggtactg ggtaccacta aagtcactta tggcgagcat gtgtttgatt
     6361 tcggcaaacc gtttgaaaaa ctcaccatgc gcgaagcaat caaaaaatat cgtccagaaa
     6421 ccgatatggc ggatctggat aattttgatg ctgctaaagc attagctgaa tctatcggta
     6481 ttacggtaga gaaaagctgg ggactggggc gtattgttac ggagatcttt gatgaagtgg
     6541 cagaagcaca tctgattcag ccaaccttta ttacggaata tccggcagaa gtgtccccgc
     6601 tggcgcgccg taatgatgtt aacccggaaa tcaccgaccg ttttgaattc ttcatcggtg
     6661 gtcgtgaaat tggtaatggt tttagcgaat taaacgacgc agaagatcag gctgaacgtt
     6721 tccaggaaca ggttaacgct aaagctgcag gtgacgacga agccatgttc tatgacgaag
     6781 attacgtgac tgcgctggaa tatggtctgc cgccaaccgc tggtctgggt attggtatcg
     6841 accgaatgat tatgctgttt acaaacagtc atactattcg cgacgttatt ctcttcccgg
     6901 cgatgcgccc acagaaataa atttcacttt aatgaacgaa gcagtcaggc gactgcttcg
     6961 ttcgtcattc cgcaaatcca gttttgagga gagacgcttc gtttcaagat aaccagcggg
     7021 tccactcaac tacagctcct aaaagagaga aaaaagtaat gccacaggca aactcattgg
     7081 ccaggttatt ccgaccagaa atgcactaag taaacgaata tgtgttttct catgtgagat
     7141 aaaccagata atgacaaagg atatgttcac accaatcaca taaaaaatta atattttaga
     7201 gaatagtgcc attttttatg ttcttcttat ccttcctggc tacttgtaaa actgacggaa
     7261 atagtcatca actgtcataa tgctgaggtc aaaatcaata tccggtaagt ctaagcattt
     7321 gagcctgatt aaatcttcga tttcacttgc ggctaacgat gtcgttattg cgaaggagtt
     7381 ttcattcaat tgtcgtggt
//
LOCUS       DACADF010000198         7373 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.200, whole genome shotgun sequence.
ACCESSION   DACADF010000198 DACADF010000000
VERSION     DACADF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.200"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(93..218)
                     /locus_tag="ISB30_004341"
                     /pseudo
     CDS             complement(93..218)
                     /locus_tag="ISB30_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            260..490
                     /locus_tag="ISB30_004342"
                     /pseudo
     CDS             260..490
                     /locus_tag="ISB30_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            542..1477
                     /gene="ttcA"
                     /locus_tag="ISB30_004343"
     CDS             542..1477
                     /gene="ttcA"
                     /locus_tag="ISB30_004343"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAP1991908.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1605..2978)
                     /gene="dbpA"
                     /locus_tag="ISB30_004344"
     CDS             complement(1605..2978)
                     /gene="dbpA"
                     /locus_tag="ISB30_004344"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAP1991909.1"
                     /translation="MTAFSTLNVLPPAQLTNLNGLGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3456..4439)
                     /gene="zntB"
                     /locus_tag="ISB30_004345"
     CDS             complement(3456..4439)
                     /gene="zntB"
                     /locus_tag="ISB30_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAP1991910.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4694..5926
                     /gene="dgcM"
                     /locus_tag="ISB30_004346"
     CDS             4694..5926
                     /gene="dgcM"
                     /locus_tag="ISB30_004346"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HAP1991911.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSEARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSTNYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5947..6510)
                     /gene="smrA"
                     /locus_tag="ISB30_004347"
     CDS             complement(5947..6510)
                     /gene="smrA"
                     /locus_tag="ISB30_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAP1991912.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSRPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6705..7358)
                     /locus_tag="ISB30_004348"
     CDS             complement(6705..7358)
                     /locus_tag="ISB30_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP1991913.1"
                     /translation="MFLGRVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWL
                     LMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERL
                     KLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALF
                     FNRGGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
BASE COUNT         1983 a         1882 c         1704 g         1804 t
ORIGIN      
        1 ttattttatt caccgaaagg tttcacaaaa tatcccctga tagaaaacta aaaaacactg
       61 cataatatcg ctattctggc acttgcgaaa aattatattc tgcacctgtt gtagcgttct
      121 atttctctgc ctggcaacga atgcacaatg tccgccacag tttctgttct aatttcttcc
      181 tgtgttaccg catcctgtac gggtagcgca acaccagcat gctaagcaag gctttcccat
      241 cagatttcac tacgactatt tgggagcgag atggaatgca gcagtaaaac gtgctggtat
      301 tcgccgccgt aatccgtacc atacgcgaca tacttttgcc tgctggctgt tgacggcagg
      361 agcgaacccg gcatttatag ccagccagat ggggcatgaa actgcgcaga tggtgtatga
      421 aatttacggt atgtggattg atgacatgaa cgacgaacag gtagccatgt tgaatgcgcg
      481 gttatcgtag ttgcaaagtt tgccctcaat atgccccatt tagtaccaga gaactgaaat
      541 aatgcaagaa aatcaacaaa ttacaaagaa agaacaatac aacctgaaca aattacaaaa
      601 acgtctgcgt cgtaacgtgg gcgaagccat tgctgacttc aatatgattg aagaaggcga
      661 tcgcatcatg gtttgcctct ccgggggtaa agacagctat accatgctgg agattctgcg
      721 caatttgcag caaagcgcgc caatcaattt ttcgctggtg gctgttaacc tcgatcaaaa
      781 gcaaccgggc ttcccggaac acgttctgcc cgagtatctt gaaacgctgg gcgttgagta
      841 caagattgtt gaagagaaca cttacggtat cgtgaaagag aagattccgg agggcaaaac
      901 cacttgctca ctgtgttctc gtcttcgtcg cggtatcctt tatcgcaccg caacggaact
      961 gggggcgacg aagatcgcgc tgggtcacca tcgtgacgat atcctgcaaa cgctgttctt
     1021 aaatatgttc tacggtggca agatgaaagg tatgcctccg aaactgatga gcgatgatgg
     1081 caaacatatc gtgattcgtc cgctggccta ctgccgcgag aaagatattc agcgctttgc
     1141 cgatgcaaaa gcgttcccga ttattccgtg caacctgtgc ggttcacagc ctaacctgca
     1201 acgtcaggta attgctgaca tgttgcgtga ctgggataaa cgttatccgg ggcgtatcga
     1261 gacgatgttc agcgcaatgc agaatgtggt gccgtcgcat ctgtgcgata ccaacctgtt
     1321 cgatttcaaa ggcattaccc acggttctga agtggttaac gggggggatc tggcgtttga
     1381 tcgcgaagag atcccactac aaccagcggg ctggcagcca gaagaagatg aaaatcagtt
     1441 ggatgagtta cggctgaatg tggttgaagt gaaataacca ggatagcgcc cgatacgcaa
     1501 gcgtatcggg ctactcttat ggaagccgat aagacgcggc cagcgtcgca tccggcaatg
     1561 ccgaagaaga tgtttactct tgcaccctgc aattcaacat ttcattattt taataaccgc
     1621 acccggcacg tttttccttt aatcttcccg ccctgtaact gtttccatgc tttatgagca
     1681 acagcctgac gcaccgcgac ataaacatgc gccggatgca cggcaatttt gccaatatct
     1741 gcgccatcaa gcccgatgtc tcctgtcagc gcacctaata catcacccgg gcgcattttg
     1801 gcttttttcc cgccatcgat acacaacgtt gccatttctg cttccagcgg cacaatggaa
     1861 ctattcgctg gcggcgtttg ccagttaagt tttatctgca acatgtcaga aatgatattg
     1921 gcccgctgtg cttcttccgg agcacagaaa ctgatcgcca gaccgctatt tcctgcacga
     1981 gctgtacgac cgatgcgatg tacatgaact tcagggtccc acgccagctc aaagttcacc
     2041 accagctcaa gcgattttat atccagacca cgcgcagcaa catcagtcgc gaccagtaca
     2101 cgggcgctac cgttagcaaa acgtaccagg gtctgatcgc gatcgcgttg ctccagatcg
     2161 ccgtgtaacg acaatgcact ttgccctact tcattcagcg cgtcgcagac agactggcaa
     2221 tcttttttgg tattgcaaaa caccacgcaa gaggatggct gatgcaagct taataaccgt
     2281 tgcaacagag gaattttgcc tttgctggat gtctcataaa attgttgttc aataggtggc
     2341 agagcatctg ttgagtcaat ttcaatcgct aaaggatcgc gctgcactct tccgctgatt
     2401 gcggcaatgg cttccggcca ggttgccgaa aacagaagtg tctgccgaga tgctggcgca
     2461 aaacggatga catcatcaat ggcgtcacta aatcccatat ccagcatgcg gtcggcctca
     2521 tccatcacca gcgtattcaa cgcatccagt gatacagtgc ctttttgcag gtgatccagc
     2581 aaacggcccg gcgttgccac gataatatgc ggcgcatgtt gtaacgaatc acgctgcata
     2641 ccgaacggtt gaccaccgca caacgtcaaa attttggtat ttggcagaaa acgcgccagc
     2701 cgacgcaatt cacctgccac ctgatccgcc agttcacgcg taggacacag cactaaagcc
     2761 tgggtttgaa atagcgacgc atcaatttgc tgtaacaagc cgaggccaaa agccgccgtt
     2821 ttgccgctgc cggttttcgc ctgcacgcga acatcttttc cggcaaggat cgccggaagc
     2881 gcggcggcct gcaccggcgt catggttaaa taacccaacc cattaaggtt tgtgagttgg
     2941 gcgggaggca aaacattcag ggtagaaaaa gcggtcacaa tctattctcg tggtcatcga
     3001 cgcaaagtca gcaggcgcgt atcctcgcag atctacgctc acgatgcgac aatttaatcg
     3061 gctcttcatc gggtggtggg tcaggcatgg gttgcgggcg agggatcgga tcgggcacgg
     3121 gaacaggatc gccaggaatc ggttcaggga caggaatttg caaataaata agtgtcgtca
     3181 tatttccctc cggtcattgg gtggactctt aaagggtaga cgctgataaa taacaggcaa
     3241 aaaaaagccg actcatcaaa gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca
     3301 aaaaaggaag taagacaata tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc
     3361 aagagagata ttgccctgaa tgggtagaga gtttattgac ttcgctcaaa ctttacggcg
     3421 tttttgtata cagacagccg ggaaaattgc ttttgttaca accatttact acgatgcaac
     3481 cataaagcaa caccaccaat aagaacaact aacagaatac aaaaaattga aaatccgaat
     3541 tgccatccac cgccagggat cccaccaagg ttgacgccaa ataaccctgt cagaaacgta
     3601 ctgggtaaaa agaccattgc catcaacgac attgtataag tacgacgagc taaattttcc
     3661 tgcatcacct gagcgatttc atccgccatc acgccagtcc gtgctataca ggcgtcgatt
     3721 tcgtcaagac cgcgcccaag gcgatcggca atatcctgca tccgacggcg ttggtcatcg
     3781 ctcatccacg gcaaacgttc actggcaaga cgagcataaa catcacgttg cggtgccata
     3841 tagcgacgca tcacaattaa ttgtttgcgc agcagagcca ggaatccacg cggtggaatt
     3901 tgctgatcga gaagattatc ttcgaggtcg ataattttat cgtgcagctg ctcgataaat
     3961 tcgctggaat gatcggtcaa cgcatcgcac acatcaacca gccacccccc gcaatcggtc
     4021 ggaccagtgc cctcttccag atcgctcacc acatcgtcca gcgccagcac tttgcgttgt
     4081 cgggtcgaaa caattaaccg cccgtccata tatacacgca tggcgaccag ttgatcgggg
     4141 cgttcatcgg tgctgccgtt tatacagcgc aatgtaatca gcgtgccttc accgagacgg
     4201 ctgactcggg gccgtgtgct ctcgcccgcc agcgcatcac gtacgttatt gggaagcagc
     4261 ggtgttgtcg ccagccattg ggcgctatca tggtgtacat aattaaggtg gagccagcag
     4321 ggatgcgctt catcaatcac atctgtattt tccagcggtt taacgccgcc tctaccatcc
     4381 agcatccatg caaatactgc atccgggaca ttaacgtccg atcccttaat cgcttccaca
     4441 gtgcctccat catcaacgca ttattttgta gtctagcctt ctggccctgt tacgcaacat
     4501 ctcatcaccc cattaccctg aaatgattaa taaaattctg tctaaattga atacaaaaag
     4561 caaaatgctt ttccgtatac aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga
     4621 attatctgat catatgacgt ggcttttttg cgatcggata gcaacaacaa ttgataaaaa
     4681 taatgggatc tcaatgatta cgcacaactt caataccctg gacttactca ccagtcctgt
     4741 ctggatcgtt tcgccctttg aggaacagtt aatttatgcc aacagcgagg cgcgactgtt
     4801 gatgcaagac ctcacgttta gtcagctacg aaccggtccc tattccgtct cctcacaaaa
     4861 agaactgccg aaatacctct ccgatctgca aaatcaacac gatattatcg aaatcctcac
     4921 cgttcagcgc aatgaagagg aaacagcatt aagctgtcgg cttgttttgc gagagctgac
     4981 agaaacagaa ccggtgatta ttttcgaagg tatcgaagca ccggcaacgc tgggtttaaa
     5041 agccagtcgc tcgacaaatt atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct
     5101 gactaactct gcgccaatgt tgttgattga cccctcacga gatggccaaa tcgttgatgc
     5161 taacctcgcc gctctcaatt tctatggtta taaccatgaa acgatgtgcc agaaacatac
     5221 ctgggaaata aatatgctcg ggcgtcgcgt catgcctatc atgcatgaaa tctcgcattt
     5281 acccggtggt cataaacctt tgaattttgt tcataaactg gcggatggtt cgactcgtca
     5341 tgtgcagacc tatgccgggc cgattgaaat ttatggcgac aagctcatgt tatgtattgt
     5401 gcatgatatt actgagcaaa aacggctgga ggagcagctg gaacatgctg cccaccatga
     5461 tgcgatgaca ggattactga atcggcgaca gttttatcac attaccgagc caggccaaat
     5521 gcagcacctc gccatcgctc aggattacag cttgttgctc atcgacaccg atcgttttaa
     5581 acacattaac gatctctatg ggcattctaa aggtgatgag gtgttatgcg cccttgcccg
     5641 cactctcgaa agttgcgctc gcaaaggcga tttggtgttt cgttggggag gcgaagagtt
     5701 tgtcttattg ctaccaagaa ccccactgga taccgcgctt tcgctggctg aaactatccg
     5761 cgtaagcgtg gcaaaagtga gtatttcggg cttaccacgc tttactgtca gcattggtgt
     5821 ggcgcatcac gaaggaaatg aaagcatcga tgaactgttt aaacgcgttg atgatgcttt
     5881 gtatcgagcg aaaaatgatg gacgcaaccg cgtgctggcg gcataagcca cggatgcgtc
     5941 tcgtgatcaa cgactgcgct tagcatggcg ctcccagttt tcttgcttcg cctgcgctgt
     6001 tttacgtagt gcgacgtaac acgccccgct gccgccatga tgcggtagcg cagtgcaata
     6061 tgcctgaaca tcatcaaatt cggtcagcca gcgcgccaca tagctgcgga caatattggc
     6121 atgcgattta tcatcccgtc ctttgccatg aataatcagc acgttacgca aaccatccgc
     6181 cagggcttgt tgaatgaaac tgaacatcat tttgcggcac tcttccaccg gctggcgcaa
     6241 aagattcagg ctcgcctgtt gcggatattt accactgcgc agcttatcca gcaccccatg
     6301 ttgcaaccct tcccgccgaa actccagcgg ccgacttagc gggatgatgt cgagaaatcc
     6361 agtggtgagg aaattatcaa gttgcagcgt gtcgatacgc tgcggcgcac gttggttacg
     6421 cgttggatgc cagtggacat ctgtagcacg tttcagcggc tggacatctt ccatggcgtc
     6481 aagaaacagc gatttgtcgt caaggttcat gtaacctcct atgcaccttc acacgtcgtg
     6541 attcagacgg gttaattgtt catacttact gcatcgttcc cttcacagat tgaaacaata
     6601 ctctacttca tacctgtgag tatctgcgca ggcggagcat tttatactag cctgttagca
     6661 gtatcgaaca gtttagcccg agtttaattc agcacaggct ccgatcagga tgatttattc
     6721 atagcaggaa ccaacaaaaa tgcgataaat aacagaccac tgattagcag aaaggtatcc
     6781 gaaaatgcca tagtttgcgc ctccctcagc atcaattgac tcaataattt ggttgaagcc
     6841 agtatttccg atgtctggtc acttcctccg cgattaaaaa acaaagcact tctggaaatg
     6901 aagtcattca ccgtatgggg aacagaaacc attttttcac ccattctcga aaaatgaaaa
     6961 ttagttcgat tattgagaat agagccacac agggcaatac caatcgcgcc cccaagatta
     7021 cgtgttaaat taaatacacc agaggctaac ttcagccgtt cttttggaat tccccccagc
     7081 gttaatgtta cgatgggggc catcgcaaac tgctgcgaaa tccctctaat tgcctgtgga
     7141 aacagtaact cctgccatcc ccattcgtgg gttatagggg taaaaagata cattgaaaac
     7201 acaaatccac ccagccccgc catcaacagc catcgcagat ttattttttt acttaaccag
     7261 aagtaaaagg gaacagaaaa taactggaaa atacctgttg tgcaaacggc aaaacctatc
     7321 tcttcggcat ttaatccgcg aacccgcccc aaaaacacag gtattaaata tac
//
LOCUS       DACADF010000199         7356 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.201, whole genome shotgun sequence.
ACCESSION   DACADF010000199 DACADF010000000
VERSION     DACADF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.201"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            85..915
                     /locus_tag="ISB30_004349"
     CDS             85..915
                     /locus_tag="ISB30_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP1991914.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            947..1882
                     /locus_tag="ISB30_004350"
     CDS             947..1882
                     /locus_tag="ISB30_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP1991915.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFARLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQLRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            1975..2217
                     /locus_tag="ISB30_004351"
     CDS             1975..2217
                     /locus_tag="ISB30_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAP1991916.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVRFIYRDIPLEDDDAA"
     gene            2207..3658
                     /gene="panF"
                     /locus_tag="ISB30_004352"
     CDS             2207..3658
                     /gene="panF"
                     /locus_tag="ISB30_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAP1991917.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLAKLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3670..4551
                     /gene="prmA"
                     /locus_tag="ISB30_004353"
     CDS             3670..4551
                     /gene="prmA"
                     /locus_tag="ISB30_004353"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP1991918.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4880..5845
                     /gene="dusB"
                     /locus_tag="ISB30_004354"
     CDS             4880..5845
                     /gene="dusB"
                     /locus_tag="ISB30_004354"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP1991919.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5871..6167
                     /gene="fis"
                     /locus_tag="ISB30_004355"
     CDS             5871..6167
                     /gene="fis"
                     /locus_tag="ISB30_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAP1991920.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6252..7136
                     /gene="yhdJ"
                     /locus_tag="ISB30_004356"
     CDS             6252..7136
                     /gene="yhdJ"
                     /locus_tag="ISB30_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAP1991921.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7220..>7356
                     /locus_tag="ISB30_004357"
     CDS             7220..>7356
                     /locus_tag="ISB30_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAP1991922.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     L"
BASE COUNT         1787 a         1790 c         1898 g         1881 t
ORIGIN      
        1 ttttcttttc gaattgtcga tatgattgaa ggataacttt caaagaaaaa tacaaggggc
       61 tatagtgtta cgcattaaag gccaatgaag agtgttaacg tgccgagaaa atcgtctaac
      121 ttactgaaac gtagattaaa aattgcagaa attgttgccg cccagggtga aatcaaagtt
      181 gatgacttat ctgcgcaact tggtgtctct ggcgtgacga tccgcggcga tctcagttat
      241 ctggagcaac agggatattt aaagcgatca tttggcggtg ctattgcgac accactctcc
      301 tctgaaccag aagcgataca accgatcgcg ccattgtctc tggcacaaca aatggaaata
      361 gcacgacatt gcgcacggtt aattcgcgat cgtgacacgc tatttcttgg acatgggaca
      421 atctgccgta agatcattcc attgctcagc gaagtgaaaa aactgcgtct gatcaccaac
      481 gatcccgaac atgcgctgct ggcaaatcag tttattgatg gcgaaataat actggctggc
      541 agtgaaatgc tgcgccccga caccgcactg gccggaaaac agcttgaaca ggcacttcag
      601 catttcacca ttacacatag cattctggaa atcagccatc tggatgctga tggtacttta
      661 aatatcaacg ttcccagact cgccgctgcc tggcagctct gtttcgatca tgcccagaat
      721 aagacggtta tcgttgccgc cgatcctgcg ccatcaacgg ctgctatcgg gcatttgcat
      781 caggcagaaa atgtgatcgt cagccgcagc attaacgaac aatatcagca acaattgcag
      841 tcggctgatt tcgttatcct ttataccagt aatgagtgct ttacctggtc caatcgcgaa
      901 aggcttcagg aatgagaggg gttttgtgac aataactatc tgcgctatgg gcgaattgct
      961 ggccgagttt ttgtcccgca acccacatca aaaattcact cagcctgggg agtttatcgg
     1021 gccatttccc agcggtgcgc cagcaatttt tgctgctcag gtggcaaaac tgtcccatcg
     1081 ggccatcttc tttggatgtg ttggtaatga tgattttgcc cgactcatta tagagcgtct
     1141 ccgtcatgaa ggtgtcatta ccgatgggat ccatgttatg aacaatgccg tcacaggtac
     1201 ggcgttcgtg agttatcaaa atccccagct gcgggatttc gtctttaata tccctaacag
     1261 cgcctgcggt ttgtttactg ccgagcacat tgataaggat ctgcttaaac agtgtaacca
     1321 tctgcatatt gtgggctcat cgttgttctc atttcgcatg atcgatgtca tgcgtaaagc
     1381 aataacgacg atcaaatcgg ctggcggcac cgtttctttc gatcccaata ttcgcaaaga
     1441 gatgctgagc attcctgaaa tggcgcaggc tctcgattat ttgattgaat atacggatat
     1501 ttttatcccc agcgaaagcg aactcccttt cttcgcgcgt cacaaaaatc tgtcagagga
     1561 acagattgtt agcgatcttc tccacggcgg cgtaaaacat gtggcgataa aacgcgccca
     1621 gcgtggggct agctattaca agcttaaaaa cggtacatta cacgcccagc atgttgcagg
     1681 tcacgatatc gaaattatcg atccaacggg tgcaggcgac tgctttggcg caacgtttat
     1741 cactcttttc ttatccggtt tcccggcaca caaggcgctg caatatgcaa atgccagcgg
     1801 cgcgctcgcc gtaatgcggc aaggtccgat ggaagggata tcctcactgg cagacattga
     1861 agactttttg cagcagcact gaacggcaat tttctgtcag attattttcc ctgcctgaag
     1921 acttgccgta gaattgcccg tttctcgtat ccccccatga ggaggacccc ttccgtggac
     1981 actcgttttg ttcaggccca taaagaggcg cgctgggcgc tggggctgac ccttttgtat
     2041 ctggcggtat ggttagtagc cgcttactta cctggcgttg cccccggttt taccggtttt
     2101 ccgcgctggt ttgagatggc ctgcatcctg acgccgctgc tgtttattgg actgtgctgg
     2161 gcgatggtga gatttatcta tcgcgatatc ccactggagg atgacgatgc agcttgaagt
     2221 aattctaccg ctggtcgcct atctggtggt ggtgttcggt atctctgttt atgcgatgcg
     2281 taaacggagc actggcacct tccttaatga gtatttcctc ggcagccgct ctatgggcgg
     2341 tattgtgctg gcgatgacgc tcaccgcgac ctatatcagt gccagttcgt ttatcggcgg
     2401 gcctggagct gcttataaat acggcctggg ctgggtattg ctggcgatga ttcagcttcc
     2461 tgcagtctgg ctttcgctcg gtattctcgg caagaagttc gcaattcttg cgcgccgcta
     2521 caatgcggtg acgctgaacg atatgctgtt tgcccgctac cagagtcgtc ttctggtgtg
     2581 gctggcgagt ttgagtttgc tggttgcgtt cgttggtgcg atgaccgtgc agtttatcgg
     2641 cggtgcgcgc ctgctggaaa ccgcggcggg tattccttat gaaaccgggc tgctgatttt
     2701 cggtatcagc attgcgttgt ataccgcctt tggcggcttt cgcgccagcg tgctgaacga
     2761 caccatgcaa gggctggtga tgctgattgg caccgttgtg ctgcttattg gcgtggtaca
     2821 tgccgcaggt ggtttaagta atgcggtaga cacgctgcaa accatcgatc cgcaactggt
     2881 tacgccacaa ggcgctgacg atattctgtc gcctgccttt atgacgtcgt tctgggtact
     2941 ggtgtgcttt ggcgttatag gcctgccgca tactgcggtg cgctgtatct cttataaaga
     3001 cagcaaagca gtacatcggg ggatcatcat cggtacgatt gtagtcgcaa tcctgatgtt
     3061 cggtatgcat ctggccggag cgttaggtcg ggcggtgatc cccgatctca ccgtaccgga
     3121 tctggtgatc ccgacgttaa tggtaaaagt gctgccaccg tttgccgccg ggatcttcct
     3181 ggctgcaccg atggctgcga tcatgtcgac aattaacgcc caactgctgc aaagttccgc
     3241 tacgatcatt aaagatctct atctgaatat ccgtccggat caaatgcaaa acgagacgcg
     3301 tctgaagcgg atgtcggcgg tgatcacgtt ggttctcggt gcgttgctgc tgcttgccgc
     3361 ctggaagccg ccagaaatga tcatctggct gaatttgctg gccttcggtg ggctggaagc
     3421 cgttttcctg tggccgctgg tgctgggtct ttactgggaa cgcgccaacg ccaaaggcgc
     3481 gctaagtgcg atgatcgttg gcggcgtgct gtatgccgta ctcgcgaagc tgaatattca
     3541 gtacctgggc ttccacccta tcgtgccctc gttactacta agtttgctgg ctttcctggt
     3601 cggaaaccgt ttcggtacat ccgtcccgca agctaccgtt ttgactactg ataaataaag
     3661 agttttgcca tgccttggat ccaactgaaa ctgaacacca ccggcgcaaa cgcggaagat
     3721 cttagcgatg cgctgatgga agcgggtgcc gtttctatca cttttcagga tacccacgat
     3781 acgccagtat ttgagccgct gccgggcgaa acgcgcctgt ggggcgacac cgatgtgatt
     3841 ggtctgttcg acgctgaaac cgatatgaac gacgtggtgg cgattctgga aaaccatccg
     3901 ctgctcggcg cgggcttcgc gcataaaatc gaacaactgg aagataaaga ctgggagcgc
     3961 gaatggatgg ataatttcca cccgatgcgc tttggtgaac gactgtggat ctgcccaagc
     4021 tggcgtgatg tgccggacga aaacgccgtc aacgtgatgt tagacccagg gctggcgttt
     4081 ggtacgggta cccatccaac tacctctctg tgcctgcaat ggctcgacag cctcgattta
     4141 accggtaaaa cagtcatcga ctttggctgc ggttccggca ttctggctat cgcggcgctg
     4201 aaactgggtg cagcaaaagc cattggtatt gatatcgatc cgcaggcgat ccaggccagc
     4261 cgcgataacg ccgaacgtaa tggcgtttct gatcgtctgg aactctactt accgaaagat
     4321 cagccagaag aaatgaaagc cgacgtggtg gtcgctaaca tccttgcagg cccattacgt
     4381 gaactggcac cgttaatcag cgtcctgcca gtttcaggcg gtttgctggg cctttccggt
     4441 attctggcaa gccaggcaga gagcgtttgc gaagcttatg ccgacagctt cgctctggac
     4501 ccggtcgtgg aaaaagaaga gtggtgccgc attaccggtc gtaagaatta accttcgcat
     4561 cgccgtatgg cgacgcagag cgaagtgcga gcgatgtcac aaaaggcacg ttaatttgcc
     4621 gattatttac gcaaatttgc gtgccaaaat tttcattcat aaagaaaaat tgagaactta
     4681 ctcaaatttc tttgagtgta aattttagtc actattttct aatatgatga tttttatgag
     4741 taattatcgc gctacgctca ttttaaatgc aattctttga tccatctcag aggattggtc
     4801 aaagtttggc ctttcatctc gtgcaaaaaa tgcgtaatat acgccgcctt gcagtcacag
     4861 tatggtcatt tcttaactca tgcgcatcgg acaatatcag ctcagaaatc gcctgatcgc
     4921 agcgcccatg gctggcatta cagacagacc ttttcggacg ttgtgctacg agatgggagc
     4981 cggattgaca gtatccgaga tgatgtcttc taacccacag gtttgggaaa gcgacaaatc
     5041 tcgtttacgg atggtgcaca ttgatgaacc cggtattcgc accgtgcaaa ttgctggtag
     5101 cgatccgaaa gaaatggcag atgcagcacg tattaacgtg gaaagcggtg cccagattat
     5161 tgatatcaat atgggttgcc cggctaaaaa agtgaatcgc aagctcgcag gttcagccct
     5221 cttgcagtac ccggatgtcg ttaaatcgat ccttaccgag gtcgtcaatg cagtggacgt
     5281 tcctgttacc ctgaaaattc gcactggctg ggcaccggaa caccgtaact gcgaagagat
     5341 tgcccaactg gctgaagact gtggcattca ggctctgacc attcatggcc gtacacgcgc
     5401 ctgtttgttc aatggagaag ctgagtacga cagtattcgg gcagttaagc agaaagtttc
     5461 cattccggtt atcgcgaatg gtgacattac tgacccgctt aaagccagag ctgtgctcga
     5521 ctatacaggg gcggatgccc tgatgatagg ccgcgcagct cagggaagac cctggatctt
     5581 tcgggaaatc cagcattatc tggacactgg ggagttgctg cccccgctgc ctttggcaga
     5641 ggttaagcgc ttgctttgcg cgcacgttcg ggaactgcat gacttttatg gtccggcaaa
     5701 agggtaccga attgcacgta aacacgtttc ctggtatctc caggaacacg ctccaaatga
     5761 ccagtttcgg cgcacattca acgccattga ggatgccagc gaacagctgg aggcgttgga
     5821 ggcatacttc gaaaattttg cgtaaacaga aataaagagc tgacagaact atgttcgaac
     5881 aacgcgtaaa ttctgacgta ctgaccgttt ctaccgttaa ctctcaggat caggtaaccc
     5941 aaaaacccct gcgtgactcg gttaaacagg cactgaagaa ctattttgct caactgaatg
     6001 gtcaggatgt gaatgacctc tatgagctgg tactggctga agtagaacag cccctgttgg
     6061 acatggtgat gcaatacacc cgtggtaacc agacccgtgc tgctctgatg atgggcatca
     6121 accgtggtac gctgcgtaaa aaattgaaaa aatacggcat gaactaattc aggttagcta
     6181 attgcttgat taaaaggcgc tactcggcat ggggaagcgc cttttttata ggtgtcacaa
     6241 agggagtgac catgagaaca ggatgtgaac cgacccgatt tggtaatgaa gcaaagacca
     6301 tcatccaggg tgatgcatta accgaactta aaaagctccc cgccgaaagt gtcgatctga
     6361 tctttgccga cccaccgtat aacatcggta aaaattttga tggtctgatc gaagcctgga
     6421 aagaagatct gtttatcgac tggctgtttg aagtgattgc agagtgccat cgcgttctga
     6481 aaaagcaggg cagcatgtac atcatgaata gcacggaaaa catgcccttt atcgatctcc
     6541 agtgccgcaa gctttttacc atcaaaagtc gcatcgtctg gtcatatgac agttctggag
     6601 tgcaggcgaa aaaacactac ggctccatgt acgaacccat cctgatgatg gtgaaagacg
     6661 caaagaacta cacgttcaac ggtgatgcta ttctggtaga agccaaaacc ggatcgcagc
     6721 gcgcgttgat caattatcgc aaaaatcctc cacagcctta caatcatcaa aaagtaccgg
     6781 gtaacgtctg ggattttccg cgcgtgcgtt atttgatgga tgaatatgaa aaccacccga
     6841 cgcaaaaacc ggaagccttg ctgaaacgca ttattctcgc ctcttccaac ccaggcgata
     6901 tcgttctcga cccgtttgcc ggtagcttta ctaccggtgc cgtagccgtc gccagcgggc
     6961 gaaaattcat tggtattgag atcaacagcg agtacatcaa aatggggctt cgacggctgg
     7021 atgtcgcgtc gcattactct gcggaagaac tggcgaaagt gaaaaaaaga aagaccggca
     7081 acctgtcaaa acgaagccgg ttaagcgaag ttgaccccga tctcattgca aagtaaagga
     7141 agtgtaagcc tggttttcag attattcatt tcgtgtatat ttcctgccag acttggttaa
     7201 acatgcacag gctcaggtaa tgattcgcaa gtattggtgg ctcgtcgttt tcgctgtctt
     7261 cgtttttctg tttgatactt tactgatgca gtggattgaa ctgctggcaa cagaaacaga
     7321 caaatgccgc aatatgaact cagttaatcc actaaa
//
LOCUS       DACADF010000200         7241 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.202, whole genome shotgun sequence.
ACCESSION   DACADF010000200 DACADF010000000
VERSION     DACADF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.202"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            485..1357
                     /locus_tag="ISB30_004358"
     CDS             485..1357
                     /locus_tag="ISB30_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAP1991923.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGYSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWEVDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1685..4813
                     /gene="ag43"
                     /locus_tag="ISB30_004359"
     CDS             1685..4813
                     /gene="ag43"
                     /locus_tag="ISB30_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HAP1991924.1"
                     /translation="MLMKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSL
                     AAVTSLPVLAADIVVHPCETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTG
                     GQWIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQW
                     VHGGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTT
                     INKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRD
                     GWQVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAF
                     SVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDIRNGGAATTVSMGNGGVLLA
                     DSGAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGT
                     LAGTTTLNNGATLTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNT
                     TLTQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATW
                     NIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQ
                     NNADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGN
                     MLQAGAFNYTLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGV
                     NGENNSVRLSIQGGHLGHDNNGGIVRGATPESSGSYGFVRLEGDLLRTEVAGMSLTTG
                     VYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTRHSMKA
                     SSDNNDFRARGRGWQGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFG
                     HGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHGVSELPVNWWVQPSVIRTFSS
                     RGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGNSAE
                     GYNGQATLNVTF"
     gene            4875..6035
                     /locus_tag="ISB30_004360"
     CDS             4875..6035
                     /locus_tag="ISB30_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991925.1"
                     /translation="MFNATFAGINSAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVE
                     TMKNKIREGKVPGVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSL
                     AAGGISFALTASVFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGM
                     LKHIDFSTASPTVRQALQKGTGAGNISALNKVMKGSLVTSLALVAVTTGPDMSKMLRG
                     RISGAQFIRNLAVASSCVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAV
                     SGKIAGALVEEDRVKILAMIQEQMTWLAGSFLLTGHEIENLNANLARVIDQNALEIIF
                     AAGIQQRAATNMLIKPLVVSIIRQRPVMEYDASHLGNMVNRLEETLPPELPA"
     gene            6111..6566
                     /locus_tag="ISB30_004361"
     CDS             6111..6566
                     /locus_tag="ISB30_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991926.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            6645..6878
                     /locus_tag="ISB30_004362"
     CDS             6645..6878
                     /locus_tag="ISB30_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP1991927.1"
                     /translation="MLGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEADAGKQLNAWLASGGLLRQ"
     gene            6979..>7241
                     /locus_tag="ISB30_004363"
     CDS             6979..>7241
                     /locus_tag="ISB30_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAP1991928.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRR"
BASE COUNT         1738 a         1948 c         2013 g         1542 t
ORIGIN      
        1 attcctatct attcgatcac ggtttggcca aaccgtgatc gattcagatg ccattttgca
       61 agtacgcatg acccggaagg gaaacactta acaacatggt atcaacagat atcattacgt
      121 gcgcactctt ataaaaatac acagcagaag caagataact ctaaacgtaa atttaatatc
      181 tcccactgac tgccccgaca atatactgaa aaatactcac ctcagtaaac ataaacacac
      241 ctgataatca ggctgcaaga tgaatatttt tctcctttac ggagtaatca gcctgctgtt
      301 taacaataac cgcgtatttt catggaagga tatcacccaa taacggataa ctcctgccct
      361 ccttcatgtt aatacgcaac atccccatcg attccaaaca atttcagaca gatattatct
      421 ccgtggatcc cccgccacct ttccggtgtg cggggttttg tcttttttca ccgggaatac
      481 atgtatgaat ccgtctgatg ccattgaggc aattgaaaaa ccgctctcct ccctgcctta
      541 ctcgctttcc cgtcacatcc tggaacatct gcgcaaactc acccgtcacg agcccgtgat
      601 tggcattatg ggtaaaagcg gggccggtaa atcttcactc tgtaatgccc tgtttcaggg
      661 ggaggtcaca ccggtcagtg atgttcacgc cggcacccgg gaagtgcagc gcttccgtct
      721 gagtggtcac ggttacagca tggttatcac tgacctgccc ggggtgggcg agagccggga
      781 cagggatgca gagtatgaag ccctgtaccg tgacattctg cctgaactgg acctggtgct
      841 gtggctgatt aaagccgatg accgggccct gtctgtggat gagtatttct ggcgacacat
      901 cctgcagtgc ggacatcagc aggtgctgtt tgtggtgaca caggccgaca aaacggagcc
      961 ctgccatgaa tgggatatgg ccggcattca gccctctccc gcacaggcac agaacattcg
     1021 cgaaaaaacg gaggcggtat tccgtctgtt ccggcctgta catccggttg tggccgtatc
     1081 ggcccgcacc ggctgggaag tggatacgct ggtcagtgcg ctcatgacag cgcttcccga
     1141 ccatgccgcc agtcccctga tgacccgcct gcaggacgag ctgcgcacgg aatctgtccg
     1201 cgctcaggcc cgtgagcagt ttaccggtgc ggtggaccgg atatttgaca cagcggagag
     1261 cgtctgtgtt gcctctgttg cacgcacggt tctgcgtgcc gtccgtgaca cagtggtctc
     1321 tgttgcccgc gcggtatgga actggatctt cttctgaacc tgttgtggat gatgtcctcc
     1381 ctgcctctaa gtatgctcac aaaagcgctg ttttcgttac tgtctctctt gtccgtgcaa
     1441 tagcttgata aaagaataaa acgatcaata cccattttac cgatcgttaa tatcgatcgt
     1501 tatgctaata ataactcctg ttagcaacgt gcgcagatac acacagacat gagattcagg
     1561 gaacaacaga gccacacgtc agaaacttcc gtcagccgga cctccggcac tgtaaccctt
     1621 tacctgccgg tatccacatc tgtggatacc ggctttttta ttcaccctca ctctgattaa
     1681 ggaaatgctg atgaaacgac atctgaatac ctgctacagg ctggtatgga atcacattac
     1741 gggcgctttc gtggttgcct ccgaactggc ccgcgcacgg ggtaaacgtg gcggtgtggc
     1801 ggtggcactg tctcttgccg cggtcacgtc actcccggtg ctggctgctg acatcgttgt
     1861 gcacccgtgt gaaacagtga atggcggaac actggtaaac catgacaacc agtttgtatc
     1921 cggaacagct gatggcgtga ctgtcagtac cgggcttgag ctggggccgg acagtgacga
     1981 aaacaccggc gggcaatgga taaaagcggg tggcacaggc agaaacacca ctgtcaccgc
     2041 aaatggtcgt cagattgtgc aggcaggagg aactgccagt gatacggtta ttcgtgatgg
     2101 cggagggcag agccttaacg gactggcggt gaacaccacg ctggataaca gaggtgagca
     2161 gtgggtacac gggggaggga aagctgccgg tacaattatt aaccaggatg gttaccagac
     2221 cataaaacat ggcggactgg caaccggaac catcgtcaac accggtgcag aaggtggtcc
     2281 ggagtctgaa aatgtgtcca gcggtcagat ggtcggaggg acggctgaat ccaccaccat
     2341 caataaaaat ggccggcagg ttatctggtc ttcggggatg gcacgggaca ccctcattta
     2401 cgccggtggt gaccagacgg tacacggaga ggcacataac acccgactgg aggggggtaa
     2461 ccagtatgta cacaacggtg gcacggcaac agagacgctg ataaaccgtg atggctggca
     2521 ggtgattaag gaaggaggaa ctgccgcgca taccaccatc aaccagaaag gaaagctgca
     2581 ggtgaatgcc ggcggtaaag cgtctgatgt cacccagaac acgggcggtg cactggttac
     2641 cagtacagct gcaaccgtca ccggcacaaa ccgcctggga gcattctccg ttgtggcggg
     2701 taaggctgat aatgtcgtac tggaaaatgg cggacgtctg gatgtgctga gcggacacac
     2761 agccactaat acccgtgtgg atgatggcgg aacgctggat atccgcaacg gtggtgccgc
     2821 caccaccgta tccatgggga atggcggtgt actgctggcc gattccggtg ccgctgtcag
     2881 tggtacccgg agcgacggaa aggcattcag tatcggaggc ggtcaggcgg atgccctgat
     2941 gctggaaaaa ggcagttcat tcacgctgaa cgccggtgat acggccacgg ataccacggt
     3001 aaatggcgga ctgttcaccg ccaggggcgg cacactggcg ggcaccacca cgctgaataa
     3061 cggcgccaca cttacccttt ccgggaagac ggtgaacaac gataccctga ccatccgtga
     3121 aggcgatgca ctcctgcagg gaggcgctct taccggtaac ggcagcgtgg aaaaatcagg
     3181 aagtggcaca ctcactgtca gcaacaccac actcacccag aaagccgtca acctgaatga
     3241 aggcacgctg acgctgaacg acagtaccgt caccacggat gtcattgctc agcgcggtac
     3301 agccctgaag ctgaccggca gcacagtgct gaacggtgcc attgacccca cgaatgtcac
     3361 cctgacatcc ggtgccacct ggaatatccc cgataacgcc acggttcagt cggtggtgga
     3421 tgacctcagc catgccggac agattcattt cacctccgcc cgcacaggga agttcgttcc
     3481 gacaactctg caggtgaaaa acctgaacgg gcagaatggc accatcagcc tgcgtgtacg
     3541 cccggatatg gcgcagaaca atgctgacag actggtcatt gacggtggca gggcaaccgg
     3601 aaaaaccatc ctgaatctgg tgaacgccgg caacagtggt acggggctgg cgaccaccgg
     3661 taagggtatt caggtggttg aggccattaa cggtgccacc acggaggaag gggcttttgt
     3721 ccaggggaat atgttgcagg ccggggcctt taactacacc ctcaaccggg acagtgatga
     3781 gagctggtat ctgcgcagtg aaaatgctta tcgtgctgaa gtccccctgt atgcctccat
     3841 gctgacacag gcaatggact atgaccggat tctggcaggc tcccgcagcc atcagaccgg
     3901 tgtaaacggt gaaaataaca gcgtccgtct cagcattcag ggcggccatc tcggtcacga
     3961 taacaacggc ggtattgtcc gtggtgccac gccggaaagc agcggcagct atggcttcgt
     4021 ccgtctggag ggtgacctgc tcagaacaga ggttgccggt atgtcactga cgacaggagt
     4081 gtatggtgct gcaggccatt cttccgttga tgttaaggat gatgacggct cccgtgccgg
     4141 cacggtccgg gatgatgccg gcagcctggg cggatacctg aatctggtac acacgtcctc
     4201 cggcctgtgg gctgacattg tggcacaggg aacccgccac agcatgaaag cgtcatcgga
     4261 caataacgac ttccgcgccc ggggccgggg ctggcagggc tcactggaaa ccggtctgcc
     4321 attcagtatc actgacaacc tgatgctgga gccacaactg cagtatacct ggcagggact
     4381 ttccctggat gacggccagg ataacgccgg ttatgtgaag ttcgggcatg gcagtgcaca
     4441 acatgtgcgt gccggtttcc gtctgggcag ccacaacgat atgacctttg gtgaaggcac
     4501 ctcatcccgt gacaccctgc gcgacagtac aaaacacggt gtgagtgaac tgcctgtgaa
     4561 ctggtgggta cagccttctg ttatccgcac cttcagctcc cggggagaca tgagcatggg
     4621 tacagccgca gccggcagta acatgacgtt ctcaccgtcc cggaatggca cgtcactgga
     4681 cctgcaggcc ggactggaag cccgtgtccg ggaaaatatc accctgggcg ttcaggccgg
     4741 ttatgcccac agtgtcagcg gcaacagcgc tgaaggctat aacggtcagg ccacgctgaa
     4801 tgtgaccttc tgacagaacc atcgcctctt tgtggtcccg atcatcatga tcgggacccg
     4861 gaccggcgca acggatattc aacgccacat tcgctggcat taacagtgcg gcattcgacg
     4921 gacagaacgg acagtatcgt tacatgggaa atcatggtcc catgcaactg gaagtccccc
     4981 gtgatcagta tgccggcgct gtggaaacca tgaagaataa gatccgcgaa ggtaaagtac
     5041 ccggtgtaac cgatcccgca gaagcgtccc ggctgattcg tcggggacat ctgacttata
     5101 cccaggcccg taatatcacc cggttcggga ccatcgaatc ggtcacttat gatattgccg
     5161 aggggtcggt tgtcagtctg gcggccggag ggatcagttt tgccctgacg gcatcggtct
     5221 tctggctcag caccggcgat cgcgatgctg ccctgcagac agctgctgtc caggcaggaa
     5281 aaaccttcac ccgcacactg gctgtctacg tcacaaccca gcaacttcac cggctcagtg
     5341 ttgttcaggg tatgctgaag catattgatt tttcgacggc cagcccgact gtccggcagg
     5401 cgcttcagaa ggggaccggt gcaggaaata tcagtgccct gaacaaagtg atgaagggtt
     5461 cgctggtgac atctctggca ctggtagctg tcacaaccgg ccctgacatg agcaaaatgt
     5521 tgcggggacg gatctccggt gcgcagttca tcaggaatct tgccgtggca tcttcctgtg
     5581 tggcaggtgg tgctgtcggg tcagtggcgg gcgggatatt gttcagtcca ctgggaccat
     5641 ttggtgcact gacagggcgt gtggttggcg gtgttctggg gggaatgatt gcctccgctg
     5701 tatcaggaaa aattgccgga gcgctggttg aagaagatcg cgtcaaaatt ctggcaatga
     5761 ttcaggagca gatgacatgg cttgccggca gtttcctgct gaccggacat gagattgaaa
     5821 atctgaacgc gaatctggcc cgtgttatcg atcagaatgc tctggagatc attttcgccg
     5881 ccggtataca acaacgggcc gcgaccaaca tgttaatcaa accactggtg gtcagtatca
     5941 tcaggcaacg ccccgtcatg gaatatgatg catcccatct cggcaatatg gttaaccgac
     6001 tggaagaaac attacccccg gagttaccgg cataacggct attctgcgcc ttttacgttt
     6061 tatcacctga tttgtcagtg tcatcgtcat gactcagaga ggtaaatacc atgattcacc
     6121 tgttcaaaac ctgcatgatt accgccttca ttctggggtt aacgtggtct gccccactcc
     6181 gggcacagga tcaacgttac atcagtatac gcaatacaga tacgatatgg ctcccgggaa
     6241 atatttgtgc ttaccagttc cggctggata atggcggaaa cgatgaagga tttggccccc
     6301 tcaccatcac tctgcaactc aaagacaaat atggtcagac gctggtgacc agaaaaatgg
     6361 aaacggaagc ctttggtgac agtaatgcca cgcgaaccac agacgcattt ctggaaacgg
     6421 agtgcgtgga aaatgtcgcc acaaccgaaa tcattaaagc aactgaagaa agtaacggcc
     6481 atcgtgtcag tctgccgtta tcggttttcg atccccagga ctaccatcca ctgctgatta
     6541 ccgtttccgg aaaaaacgtt aactgatccc tgaagtcagt ttcatgcttc agcctctgtc
     6601 acaacgcgtg gcagaggctt tttatttatc aggagaaaac agatatgctg ggttacacag
     6661 agtatgtcct ggctgaagga tctttctcat acgggcaggc ggttgctgtc ataaccgctt
     6721 accgcaatgt gtttatccag gatgacccgg gaatgcattt ccgtcgggtt atccgcaacg
     6781 cggaaggtca gcgacgctgg cgctgccgga actctgaagc tgatgccgga aaacagctta
     6841 atgcgtggct cgccagtgga gggcttctca gacaataaac ctcattgatt tcatccatca
     6901 ggccgcgtct tctccgggag acgcggcttt tttcatttat tacaccagta aatcttaacc
     6961 accgataagg agcaaattat gcgactagcc agtcgttttg gttatgcgaa ccagatacgc
     7021 cgtgaccgtc cgctgacaca cgaagaactg atgcactatg tgccggggat tttcggggaa
     7081 gataaacaca cgtcccgaag ccagaactat acgtacatcc ccaccatcac cgtactggaa
     7141 agcctgcagc gggaaggctt tcagccattc ttcgcctgcc agacccgtgt gcgcgacccg
     7201 ggccgccggg gatacacaaa acacatgctg cgtctgcggc g
//
LOCUS       DACADF010000201         7153 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.203, whole genome shotgun sequence.
ACCESSION   DACADF010000201 DACADF010000000
VERSION     DACADF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.203"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..519
                     /locus_tag="ISB30_004364"
     CDS             <1..519
                     /locus_tag="ISB30_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAP1991929.1"
                     /translation="ILLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNV
                     VDRVIEGAYEVVGVFDRIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTAD
                     ILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRAL
                     WVMAETLLESLR"
     gene            574..1059
                     /locus_tag="ISB30_004365"
     CDS             574..1059
                     /locus_tag="ISB30_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP1991930.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVALEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            1075..1551
                     /locus_tag="ISB30_004366"
     CDS             1075..1551
                     /locus_tag="ISB30_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAP1991931.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1614..1835
                     /locus_tag="ISB30_004367"
     CDS             1614..1835
                     /locus_tag="ISB30_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAP1991932.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1854..2498
                     /locus_tag="ISB30_004368"
     CDS             1854..2498
                     /locus_tag="ISB30_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HAP1991933.1"
                     /translation="MSSIITITVYTLDELSCPARGKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQPATPRKIR
                     EYAPQDRELHRIADTLQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQAMT
                     TDAEAAVSEALRDLAFWLYRQLENKYDWLTSDTAVDEALLINEYTFTEAGLRAG"
     gene            2548..2916
                     /locus_tag="ISB30_004369"
     CDS             2548..2916
                     /locus_tag="ISB30_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAP1991934.1"
                     /translation="MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCDYVYLAVYPTPEMKN"
     gene            3005..3382
                     /locus_tag="ISB30_004370"
     CDS             3005..3382
                     /locus_tag="ISB30_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP1991935.1"
                     /translation="MKTLPDTHVRAASCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKHPEAKR"
     gene            3379..3867
                     /locus_tag="ISB30_004371"
     CDS             3379..3867
                     /locus_tag="ISB30_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991936.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVELAELINVVCDNGYS
                     LRVVDESDRTSTDSIPPSAALSGIRCSTAHITETDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASTECLPDLPTF
                     DW"
     gene            3884..4081
                     /locus_tag="ISB30_004372"
     CDS             3884..4081
                     /locus_tag="ISB30_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAP1991937.1"
                     /translation="MKSLTTETALYILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IKQAREDIRTLRQLQLLQQNR"
     gene            4166..5011
                     /locus_tag="ISB30_004373"
     CDS             4166..5011
                     /locus_tag="ISB30_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HAP1991938.1"
                     /translation="MSDITISRPELVNGHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQHS
                     GMLSLMDAQAHDTWYRSLEYDNFPEISEANILSTFEQLHQNKDDVFERGVINLFRGLS
                     WNYKTNCPCKFGNKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKACYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPN
                     VLADR"
     gene            5080..5475
                     /locus_tag="ISB30_004374"
     CDS             5080..5475
                     /locus_tag="ISB30_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HAP1991939.1"
                     /translation="MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEG
                     KLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITEALERLNIRYRLAGATECYNKSQ
                     STQIPVSLGIKTPSRFKRMLIVGKSKINA"
     gene            5468..6401
                     /locus_tag="ISB30_004375"
                     /pseudo
     CDS             5468..6401
                     /locus_tag="ISB30_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536504.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
     gene            6833..6988
                     /locus_tag="ISB30_004376"
     CDS             6833..6988
                     /locus_tag="ISB30_004376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991940.1"
                     /translation="MAALVVTWFNPVKEAFYMHLPAAGKTKKVALVDCMRKLLTILNA
                     MLRKNEE"
BASE COUNT         1892 a         1841 c         1756 g         1664 t
ORIGIN      
        1 attctgctca actctcatga cggtacctcc agctaccaga tgctgccggg ttacttcagg
       61 ttcgtctgcc agaacgggtg cgtctgtggt cagtctctgg gggaagtgcg tgttccgcac
      121 cggggaaatg tggtggacag agtcattgaa ggggcttacg aggtagtggg cgtgtttgac
      181 cggattgagg aaaagcgtga tgccatgcag tcgctgattc tgccgccacc ggcacgccag
      241 gcgctggcac aggcggcact gacttaccgt tatggtgacg aacatcagcc cgtcaccacc
      301 gccgacattc tgacgccacg acgccgggag gattacggta aggacctgtg gagtgcttat
      361 cagaccattc aggagaatat gctgaaaggc ggaatttcag gtcgcagcgc taaaggaaaa
      421 cgtatccata cccgtgccat tcacagcatt gacactgaca ttaagctcaa ccgcgcattg
      481 tgggtgatgg cggaaaccct gctggagagc ctgcgctgat tcagccatta ccctgaaaac
      541 cacgcattta tttgtccttt aaggagtcat ttcatgaaaa ccatttcaca taattccacc
      601 acatcttctg tttccgtaac cgctgcatca gggaatgacc agcctcagtt ggttgccacc
      661 atagtccctg atgaacagcg catcagcttc tggccgcagc attttggcct cattccacag
      721 tgggtggccc tggagccccg tgttttcggc tggatggacc gtctgtgcga agactactgc
      781 ggtggtatct ggaatctgta caccctgaac aacggcgggg catttatggc ccccgaaccg
      841 gatgacgatg atgacgaaac atgggtactg ttcaatgcca tgaacggcaa ccgcgctgaa
      901 atgagcccgg aagccgctgg cattgctgcc tgtctgatga cgtacagtca tcacgcctgt
      961 cgtacggagt gttatgccat gacggtccat tattaccggc tgcgggatta cgccctgcag
     1021 catccggaat gcagcgccat tatgcgcatc atcgactgaa tggaggcagg gacaatgcag
     1081 cagctttcct ttctgcccgg agaaatgacg cccggcgagc gcagtctcat tctgcgggct
     1141 ctgaaaaccc tggaccgcca tcttcatgaa cccggtgtgg ccttcacctc cacccgtgcg
     1201 gcacgggaat ggctgattct gaacatggcg ggactggagc gtgaagagtt ccgggtgctg
     1261 tatctgaata accagaatca gctgattgcc ggtgaaaccc tcttcaccgg caccatcaac
     1321 cgcacggaag tccatccccg ggaagtgatt aaacgcgccc tgtaccacaa tgccgctgcc
     1381 gtggtactgg cgcacaatca cccgtccggt gaagtcacac ccagtaaggc agaccggctt
     1441 atcaccgaac gtctggtaca ggcactgggc ctggtggata tccgggtgcc ggaccatctg
     1501 atagtcggtg gcaaccaggt tttctccttt gccgaacatg gtctgcttta acccgtcaca
     1561 accacatcac acctgttttc acttttatct tctgtcttca gaggtatccc attatgaaaa
     1621 ttatcactcg tggtgaagcc atgcgtattc accaacaaca tccgacatcc cgtcttttcc
     1681 cgttctgtac cggcaaatac cgctggcacg gcagcactga agcgtatacc ggtcgtgaag
     1741 tgcaggatat tcccggtgtg ttggccgtgt ttgctgaacg tcgtaaggac agttttggcc
     1801 cgtatgtccg gctgatgagc gtcaccctga actgaatcag gacgggcatt cagatgagca
     1861 gcattatcac catcacagtg tatacgctgg atgaactttc ctgcccggca cggggaaaag
     1921 cccgcgactg gtaccgtcag catcatgctg acagcaactg gtatgaaaac gtgtatgaag
     1981 acttcaggga ggtctgtggc attttcggga ttgacctccg ccagcgcgtt ttccggctca
     2041 gcaatggcag gtttatggaa gagccgtgca tctggttcag cggattctgc agccaggggg
     2101 acggagcctg ttttgaaggg cgctggcact ggcagccagc gacaccccgg aaaatcaggg
     2161 aatatgcccc tcaggaccgt gagcttcatc gtatcgcgga taccctgcag gcggtgcaga
     2221 aacgtaactt ctggcagctt caggctgaaa tcagtcaccg gggacgttac tgccatccgt
     2281 acagcatgga tatcaccgtc acccggaaca gtccgactgg tcaggcaatg acaaccgatg
     2341 cggaagccgc tgtcagcgaa gccctgcgcg acctggcctt ctggctgtac cgccagctgg
     2401 aaaacaagta cgactggctg acctcagaca ccgctgtgga tgaggccctt ctcatcaacg
     2461 aatatacctt caccgaagcc ggtctgcgcg ccggctgacc cgtcatcgtt aaactatcat
     2521 tcaccgattt tctgtgagga ttttatcatg tcggacacac tccccggaac aacgcttccc
     2581 gacgacaaca aagacctccc ctggtggggg ctgccctgca ccgtgacgcc ctgtttcggg
     2641 gcctgtctgg tgcaggaggg taaccggttg cattaccttg ccgaccgcgc cggtatcaga
     2701 ggccggttca gcgatgcgga tgcgtaccat ccggaccagg cctttccgct gctgatgaaa
     2761 caaccggaac tcatgctcac cagcggtgaa ctgaatcccc gccatcagca taccgtcaca
     2821 ctgtatgcga aaggactgac ctgcgaagcc gataccctgg gctcctgtga ttacgtttat
     2881 ctggctgttt atccgacgcc cgaaatgaaa aattaactct ccagaatagc cttctgctac
     2941 ggcctggtgt tttcaccacg ccacttttcc atttttatat ctgcatatca ggaaaatctt
     3001 cagtatgaaa acattacccg acacacacgt acgggcggca tcgtgctgcc cgtctcccgt
     3061 caccatctgg cagacactgc tcacccgact gctggaccag cactatggcc tcacgctgaa
     3121 tgacacaccg ttcgctgatg aacgtgtgat tgagcagcat attgaggcag ggatttcact
     3181 gtgtgacgcg gtgaactttc tcgttgaaaa atacgcgcag gtacgtaccg accagccggg
     3241 attcagcgca ggagccccgt cgcagttaat caacagcatt gatattctcc gggctcgcag
     3301 ggcgaccggc ctgatgaccc ggaacaatta cagaatggta aataacatta cccagggcaa
     3361 gcatccggag gcaaaacgat gaaactggcc ctgacgctgg aagctgacag cgttaacgta
     3421 caggcactga acatggggcg aattgtcgtt gacgtcgatg gtgttgagct tgccgaactg
     3481 attaacgtgg tctgcgataa tggctactcc cttcgtgttg ttgatgaatc tgaccggacc
     3541 tcaacagaca gcataccccc ctctgctgcc ctgagcggta tacgctgtag taccgcgcat
     3601 atcacggaaa cagacaacgc ctggctgtac tcgctgtcgc atcagacgaa tgacaccggc
     3661 gaatcagaat ggattcattt tacaggtagc ggatatctgt tacgtaccga tgcgtggtca
     3721 tacccggttc tgcggcttaa acgcctgggg ctgtcaaaaa cgttccgccg tctggttgtc
     3781 acacttaccc gacgttatgg cgtcagtctc attcatctgg atgccagcac tgaatgcctg
     3841 ccggatttac ccactttcga ctggtaaccc ggaaattcac aacatgaaat cattaaccac
     3901 ggaaacagcg ctgtatattc tgattgcgtg gctgcaggac aatattgact gcgaatcagg
     3961 gattatcttc gacaacgatg aggataaaac ggattcagca gcactgctgc cctgcattaa
     4021 acaggccagg gaagatatcc gtaccctgcg ccaactgcag cttctgcaac agaaccggtg
     4081 agtcttactc accagacttc attccactga cgccagcctg aatacggctg gcgttttcgt
     4141 ttataaacca aaaggaaaaa cgatgatgtc agatatcaca atctcccgcc cggaactggt
     4201 caacggacat acggacgtta tctgctcaac cagtatccgc cacattctgg ctgtacgaaa
     4261 gagtgcgctg ctgcaaatcg acacacttat ccggcaactg gcagaaatct cagtactgac
     4321 agaaagtatt ggaggtaaaa ccgccctgga ctgggcgatg aaacaggatt ttcgctgcgg
     4381 ctgctggctc atggagaaac cggaaaccgc aatgaaagcc atcacccgca atctcgatcg
     4441 cgaaatctgg cgtgacctga tgcaacattc cgggatgctg tcattaatgg atgcgcaggc
     4501 ccatgataca tggtaccgct cactggagta cgataacttt ccggaaatca gcgaggcgaa
     4561 tatcctgagt acgtttgaac aactgcacca gaataaggat gacgtgtttg agcgaggtgt
     4621 gatcaacctc ttcagagggc tgagctggaa ttacaaaacc aattgcccct gtaaatttgg
     4681 caataaaatt atcgtcaaca atctggtgag atgggaccga tggggattgc atcttatcac
     4741 cgggcaacag gcagatcgac tcgctgacct ggaaagaatg ctgcatctgt tcagcggcaa
     4801 accgatcccc gacagccggg aaaatatcat cattcggctg gatgatcaca tccgctccgt
     4861 tcagggcaaa gcgtgctatg aagatgagat gttcagcatc agatacttta agaagggctc
     4921 cgcacacatc acgttcagga aaccagaact ggttgacagg ctaaatgaca taattgccag
     4981 gcactatcca aatgtattag cagacaggta atgtggcctt gccccgctat taaacatgtc
     5041 atatttttgc tatcattgtg aaaatttttc ggaggaaaca tgactacaaa agaccgtatt
     5101 cagtcccgac tgaaccgttc aaaacgttat gtgttcaccc gggatgattt cagggatatc
     5161 gctggctatg atcaggttgg cagagctctt cgtacactgg taaacgaagg taagttgatg
     5221 aaagtgggct atggtgttta caccaaagcc cgtcgtaaca tcatcactgg gaaaatcatg
     5281 cccgcagcgc ctgggggctc tgctgcagta attacagagg ccctggagcg cctgaatatc
     5341 agataccgtc tggcaggagc aacggaatgc tacaataaaa gtcagtccac acaaattcct
     5401 gtatctctgg ggataaaaac accttctcga tttaaaagaa tgttaatagt agggaaaagt
     5461 aaaatcaatg cctgaattaa catcattatt tcctgacgtt gccgatgcac tggatattga
     5521 atctgtcgct atcgtcgaaa aagattattt tgtggttgat cttttaagat tattaaaaga
     5581 aataaaacca gaaacacata ctctcgtgtt tgcaggaggt accgcattat caaaagcagg
     5641 tatctcgcta aacagaatgt cagaagatat tgatataaag ctcgtgccaa cagagaactt
     5701 tatgcaaaat ggcagagaca agagaagaaa aattcgcaag gaaatcgtgc aaataatcac
     5761 ggatgttatt cataattctg acattttcag cttggataat gaaaacgccc gtataacacg
     5821 tgatgagtat cgttataacg aaatctcggt acgttatccc cagacatttg cacaagtccc
     5881 ctgtcttcgt ccatttatta agctggagtt gatggaaagc actctgctgg aacatccgga
     5941 aagcagagat atttattccc ttgtcacgga gttaacgggt aaaggtacac ctgtaacagc
     6001 attcccctgt gccaccattt tatccactca ggctgaaaag ctcatctcca tgatgcgaag
     6061 aacagcagca catcttcgta atcccgaaca acaggatgat gagtttctgg tacgtcacat
     6121 ttacgacaac tactgcattg tcagggaaaa aggtgtgaat gttcctgtat tgaaaaattt
     6181 tgttcagata tgtattcagc tatggtaacc agtacccaca attttgtagc tcaccagttg
     6241 atgaacttaa gaaagggctg gatgcactcc gggattatcc tgtacacaaa ttgcgctttc
     6301 aacgatttgt taaaccgatg gtatttggta atacacagat taactgggaa gaagcctatt
     6361 ccagtttcag acaaacagcg ctaagtgttc tgaccagcta acaaacgcgt taagtacgat
     6421 tccacaattc agcatatcga accagaccaa atgattacag caaagcacaa aaaagaggga
     6481 ttatagcgtc aaaagaattc gaattgcaca tgggggcctc attggtggcc tcaaaaacaa
     6541 caaatacaaa ataatctata taaaacaatt tgttatacaa tcagtttcat tccgattccg
     6601 ggcaccacta attcttaaga acccgcccac aaggcgggtt tttgcttttg gatctttata
     6661 taaaataaac ttgggagcat aacgccatca ggtcttaaac actcccgaat catcaataaa
     6721 catcccccgc taacaaaaca cagttcagtt cacataacat ccctgttatc ccacacatat
     6781 ctgctggccg aacgacgcca gtgcgcgtac ggaggcaaga gcagccctgc acatggcagc
     6841 gcttgtggta acctggttta atccggtgaa agaggcgttt tacatgcatc tgccagcagc
     6901 cggaaaaacc aaaaaagtgg cgctggttga ctgtatgcgt aaattgctga ccatcctgaa
     6961 cgcaatgctc agaaagaacg aagagtgaaa tgaatcgtag catcatgttg ctccataatt
     7021 tttacgttca agacagttga ttatcggcgc atatgactca aaaagagcct gatgattgcc
     7081 tcttttccat aggctctttc tctgaagtga aggaagaccc ggaagaaagc actcgctggc
     7141 atacgtgact cca
//
LOCUS       DACADF010000202         7145 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.204, whole genome shotgun sequence.
ACCESSION   DACADF010000202 DACADF010000000
VERSION     DACADF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.204"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            89..520
                     /gene="osmC"
                     /locus_tag="ISB30_004377"
     CDS             89..520
                     /gene="osmC"
                     /locus_tag="ISB30_004377"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAP1991941.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(710..3109)
                     /gene="dosP"
                     /locus_tag="ISB30_004378"
                     /gene_synonym="pdeO"
     CDS             complement(710..3109)
                     /gene="dosP"
                     /locus_tag="ISB30_004378"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HAP1991942.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGASFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(3134..3351)
                     /locus_tag="ISB30_004379"
                     /pseudo
     CDS             complement(3134..3351)
                     /locus_tag="ISB30_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(3675..4994)
                     /locus_tag="ISB30_004380"
     CDS             complement(3675..4994)
                     /locus_tag="ISB30_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAP1991943.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNNSNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(5125..6660)
                     /gene="gadC"
                     /locus_tag="ISB30_004381"
     CDS             complement(5125..6660)
                     /gene="gadC"
                     /locus_tag="ISB30_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAP1991944.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(6816..>7145)
                     /locus_tag="ISB30_004382"
     CDS             complement(6816..>7145)
                     /locus_tag="ISB30_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP1991945.1"
                     /translation="AKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRL
                     RGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQ
                     QNSFKHT"
BASE COUNT         1830 a         1749 c         1780 g         1786 t
ORIGIN      
        1 gccggatttt attcggatta tcctgcttat cctcgtgctg tttctcacgt agtctataat
       61 ttccttttaa gcccacagga gagcaacaat gacaatccat aagaaaggtc aggcacattg
      121 ggaaggcgat atcaaacgcg ggaagggaac agtatctact gagagcggcg tgctgaacca
      181 acagccgtat ggatttaaca cgcgttttga aggcgaaaaa ggaaccaacc ctgaagaact
      241 gattggcgca gcgcatgccg catgtttctc aatggcgctt tcattaatgc tgggggaagc
      301 gggattcacg ccaacatcga ttgataccac cgccgatgtc tcgctggata aagtggatgc
      361 cggttttgcg attactaaaa tcgcactgaa gagcgaagtt gcggtgccgg gtattgatgc
      421 ctccaccttt gacggcatta tccagaaagc aaaagcagga tgcccggttt ctcaggtact
      481 gaaagcggaa attacgctgg attaccagtt gaaatcgtaa agcgttgccg gatgacgcgt
      541 caggcgcgtg aatgcctgat gcgtcagcac aatcacatca ggctgagaag ggattgttct
      601 gacatggcta tgcgttgtcg ttgtccaact aaacgtagcg gaaatcacag catttaagtg
      661 acagtgtcac gttaaataaa aacccgcgag tgcgggcgag aggaatttat cagattttca
      721 gcggtaacac gctgctcatc cagcctggaa tttcttcggc gggcaggggg cgggaaaaga
      781 aatatccctg aataacgcga cagtggatct tgcgtagcat ctcaaattgc tctttggttt
      841 cgacgccttc cgccacgacg gttaaattga ggctttgccc aatgctggta atggcttcga
      901 gtaaggcaag gatgcgtttt tcagtcagac aacgatcgac aaaacttttg tcaattttga
      961 tttccgttac cggaagactg actaagcggg ataatccgga aaagcccgta ccaaaatcat
     1021 ctaccgataa accgatgccc atatcgcgca ggatctgaat gcgcttaaag atttcggtat
     1081 cgtgttccat catcatgctt tccgtgattt ccaccgtcag ctggtggccg tcaatacccc
     1141 aggcgtgcat tgcatcagat acctgattag gcagctgatt actgcgaaag tgcagcgcag
     1201 acaagttaac ggataacgcc gggatatgaa tattctggct acgccattct gctaactgac
     1261 ggcaagcttc cgcgatgacc cagcgcccaa tattttcgat ttcaccaatc tcttctgcga
     1321 gaggaataaa ccgtgaaggg ggcacatgac catgcagggg atcgtaccag cgagcaaggg
     1381 cttcgatgcc gtacagttca cccgtttctg cgaagatttg cggctgataa accagtttaa
     1441 gttggttatt gctaatcgct tcttttagcg ctgcgcctaa aaccaaacgc tcttttacca
     1501 tttcgttcat cgccgggctg aagaactgcc agccgttacc gccatttttg cgaataaaat
     1561 ccattgcatt gtgagcagtg gagagcaggt aatcgcggtt tttacccacg tcgtagctga
     1621 tgccaatgct caaggtgagc gggaatggtt tatcgtcaat cattatcggc ttgctgacca
     1681 cattccgtag ctcatcggcg atctgggtaa tgttactgac atcgttttcc aggctcacga
     1741 ggacaaaaga cgcaccttca atacgacaga gatactgatc cggtttgagt ttttcacgaa
     1801 agcgattgac cacttccagc aatgcctgat cggcccacgc atagccaagg ctatcaatca
     1861 catcctgaat atggtcaaca ccgatgagat acaccaccgg agagacggct ttgtcgacca
     1921 ggtcatcgag gtaattgtgc aggttattgc gatttggcag accggtcatc ggatcaaatt
     1981 ggatgagttg ttcaatatgc tgacggcttt tttcctgttc cagcgccagc gcggccatat
     2041 gctggctgat atctgccacg cgttcgataa aggcgctggt ttctgctcct gacgaggttt
     2101 taatttgcag gatccccgca ggcgcgccat cgcgctgacg aatggtcgct gaccagcttt
     2161 gcgcattttg aacttctgca ccgtgcgaag atgacgccca gtgtatcggc atcccgttgc
     2221 gcagcgcgaa cagagaaaca tgcgattcgt tgagaacaga ttcgatatta cgacaaatga
     2281 tttcccccat ttcatgaaat ggtgggctgc tgcacatggc ggcgagaata ttgccttcaa
     2341 gctggcggat ttgcctttct tcggtgatat ccgagaatgt cattaccagg ttctgcagat
     2401 gcgcgagcac gtcataaacc gggctgatag aggctttaat ccagattttt tcaccggtgc
     2461 gcgtcaacag cagaaattcg tcctgatcgc gggcggtttt ccatagcaac tgctgtaaac
     2521 gaatacggtt atcagcaggg aattctggaa tgttcaggag tgtatcgggc tgcataccgc
     2581 tggcttcgtt aatgcagtaa ccaaacattt cggtaaatgc gcgattgcac tgcacaatat
     2641 ggcgttccgg atcgaggaca atcaccggtc ggtcgagatg gtcaacggca ataatcaatt
     2701 gtcgggtctg ttctttttgc gccatttcta cgctggcatc ccgtaccagc gccaggtaat
     2761 aaactttccc ctcggcgctc actttcgata gcgcaaaacg ggtccagatt ttactgccgt
     2821 cttttttctc cagctgcagc tcccgactca tcccctcgac gcgcgcttta ccaccttcac
     2881 ggttgtgacg aatgtattga ggatgcgcag gacgcaaatc ccgcggaatc agcatatcaa
     2941 tgttattgcc aatgacttct tcacgtttgt acccccagag cttctctgcg gcggggttga
     3001 aaaacatcac ttcatcattt tcgttaatta acaccgcgcc catcatattt tgctcaaggg
     3061 cggggaaaaa aatgccatcg gcggcattat ccgcatcggt tagcttcatg attacctctg
     3121 catcctggcg catctaaaga ctggctttac agagttcaac acggtttcta cctcgtcttt
     3181 tgcgatatac agagcttcat cggctatttg aatgaggcgc tcatagtcag gatgaccatt
     3241 aaacatggcg gcaccgatgg aaagtgagag ggcaatatcc tcgccgtttg cggctttaaa
     3301 ataatgtcga cctttatggt taaaccgcag gccaaaatca accaaacaca acgtgcggga
     3361 ttggctaact gcaccatatt ctcctgaaat atgaagatat actgaaaaga aataagcgac
     3421 ttaggacagt ttcaatctac gctactgttc ttcagaagag tatagcccat cgtaattatt
     3481 tttcggtgac agcgaatatc gtatgttttt tcatattcat acatttttat tagggggatt
     3541 atggctgttt aactaagtgt ggttaattgc gtggttaatt tgacgtattt aagcatgatt
     3601 gctagtggga tagttcaaga gggtaacaag ccagtgggta aagcaccggc ttgttacaaa
     3661 gtaagaatgg gagtttaact gccccaacga ctttgcagat agctgaccgc ttgttgagtc
     3721 tgcggtttat tcagatagtc ctcacggaac aagattgtgc cgctaatttc cggcacagca
     3781 tcgttaagat cgagctgctt tttcagttcc ggtacgccgc cgttaatcat ccagtctggc
     3841 tctatctttg acggttctcc tactttatag aaggcgatac cgatatacag gcgggtcctg
     3901 gtcggtttaa cgacatccgc ccaccatttt gccaacacgt cataacgcgc ggcactccgt
     3961 gagaatggcc agtaaatttg gggagcaatg taatccagca acccttgttc cacccatcga
     4021 cgggtatcag cgtaggattc gtcataggct gccgcgcctc gggtatcgga accgagcgga
     4081 tcgtgtgatc ggttacgcca cacgcctgcc gggctaacgc caaattcgac ttccggctta
     4141 atgcttttaa tggtgtgcga aaccttcgca attaactgct gggtattgtt gcgccgccag
     4201 tctgcttttg acgcaaatgc gcctccgtat ttgcggtacg tttcgttatc atttagccgt
     4261 gaacctggtg actcagtata gaaatagtca tcaaactgca cgccatctac cggatagcgg
     4321 gaaaccactt ccgcaactat tgatgtgatc cagtcctgaa cctcagggat gcccgggtcg
     4381 aggacaaagc gatcgccgga cgttctgatc cagtcgcggt gttgcacata gacgctggcc
     4441 ggttgttgag acagggtgct attcagttcc ctgatagtac cgggcttcgt attaaccgat
     4501 acgcgatagg ggttaaacca ggcgtgtact ttcatcccac gcttgtgggc ttcgtcgagc
     4561 ataaattgca gcggatcgta acccggattt tcaccaatct taccggtcat aagatcggac
     4621 cacggcaaaa ttttcgatgg ccacagggcg gtaccatccg gcttgacctg aaaaaagacc
     4681 gtgtttatgc cgagacgttg cagatgatcc agtttgtcga tcatcgcctg ttgttgtaca
     4741 cgtgcccggc tggtggggtt actattgtta accgaggaaa ccggtggcca gtcgaggcga
     4801 gacaccgtgg ccagccagat gccacgcatc ggttgtgacg attgttgcgt cgtggctggt
     4861 ggctttgagc ccgctggtgg tgtcaccatg gactctggag gcgtgctttt acaactacac
     4921 agtaaaagtg ccagtgcaac cagtatcgct ggtcttctaa tcgctaattt ctcgtttcgg
     4981 gagcagatat ccatatgtgc tggtttccgg taaacagatg tgcgctcatt ctcgtactta
     5041 tcccggttaa gtcaatacga caacaagcac gaaaaaggga gcgatgaatt atcgctccct
     5101 tgtcttataa gcattcagac atggttagtg tttcttgtca ttcatcacaa tatagtgtgg
     5161 tgaacgtgca cgcgggtgca ggaagaagtg accttttggt gcgttctgac tgttgattgg
     5221 ctccagagtg acgccggtat tcgctttgct tttacgatca tgaacagcat agagaataaa
     5281 gggcagggca agtaccacca ggaaactaac gaccagtaat tcaacataca tatcggtaga
     5341 gtcaccctgg atgttatccg gcggcaggaa ggaaacaata aacgccataa ttgaagtcag
     5401 cagaccgaca atagccacga ccagtttcac ccctttacca ccagggatat taaatgtgcg
     5461 ttttaagtca ggatgtttaa gcaccaacac aatgtagcca ataaacagca tgaaataagc
     5521 acacagataa atcaccaccg tcagcgccag tgcgatcagg aaggacatgt tgttaccgcc
     5581 accggtattg gtgaggatga tcaacgcgat agaagtaatc accaactgcg aaatgaccag
     5641 cgttaccggt acgccatttt tgttcatttt agcgaatgcc gctggcagca ggtttttctg
     5701 cgctgtgaca tacatcccgc gagaaggacc aacaatccag gaagcgattt ccgccagaac
     5761 acccagcaac agcagtgcgg agatcacgcg aaccgtccac tcaatttccg gtgccacatg
     5821 tgacatcaga acggtaaagg tttgcattac ccctgcggag aggttgattt cattacccgg
     5881 aatgaccatc gcaatagaca aaccgccaac agagcttagg cagattgccg ccaccatcag
     5941 cagtaacata gccagtggat agtcgcgccc ggggttgctc atttcattga cgtgggttgc
     6001 ggaagcttca acgcccatat aactcaaaat gaaggcaaca aatacaacca gggtgcccac
     6061 tttagagaag tcagggaaga aggtcttcgc atccatttcg atagcaacgg gggcaccgga
     6121 gtgcagataa atagccgcta atgcgatcaa aataaatgca ggtaacagga taccggcgaa
     6181 gaagccaact ttagcaattc gcgccgtgta tttcgtgcca ccaaactgcg ttaatgccag
     6241 cgcccaaaga atgatgagtg ctgcaatagt tttggtaatg gggtcttcat tcagcgctgg
     6301 ccatttcagg atgtaggaga gtgcccctaa cacgaaatag agcatcggaa taaaaccaat
     6361 ggcgatttgc agatagccaa atgagatcgc tgcaaatccc catctcggcc ccagagtatt
     6421 tgatacccag gcgaagacgc caccttcttc ccagccgtcg acggtagcca tttccgcagc
     6481 acaaagtccc acgggaataa accataaaat cccgcctaat agcaggaaga agactaatga
     6541 aaagcccgat gttgcgaagg tagggtattc ataaacagcc attaccatcg atgccgttat
     6601 ggcaaaaaat ccgagtaatg tgagctgctt agctttacct gtctgtactg atgtagccat
     6661 attatccccc taaaacggta ttcctgtcgg aaccgcacct gttttgttct gaagcgtatt
     6721 cagaacaata ttttccgttg ctaatgccag tgaacagact ttggaaattg tcccgaaacg
     6781 gcttcgtttc gggacaccgt taccgttaaa cgttatcagg tgtgtttaaa gctgttctgc
     6841 tgggcaatac cctgcagttt cgggtggtcg ctgagatatt tcagggaggc tttgtagtct
     6901 tccagcaaca gttcagcaaa gtccatttcg aagccgcgac gacacataat gcgcatcacc
     6961 acgatgtcgg tggcttcacc gccgagagtg aaggccggaa cctgccagcc gcgcagacgc
     7021 agacgttcag agaggtcgta cagggtgtat cctggatctt caccatcttt cagtttgaag
     7081 caaaccgccg ggatgccttc gtccgggcga ccggtacaga tgaactcata cggccccagt
     7141 ttggc
//
LOCUS       DACADF010000203         7116 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.205, whole genome shotgun sequence.
ACCESSION   DACADF010000203 DACADF010000000
VERSION     DACADF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.205"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            341..1255
                     /locus_tag="ISB30_004383"
     CDS             341..1255
                     /locus_tag="ISB30_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter substrate-binding protein"
                     /protein_id="HAP1991946.1"
                     /translation="MPRIITRPFLFSPLTLCISAVVSAAKTMVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            1275..2612
                     /locus_tag="ISB30_004384"
     CDS             1275..2612
                     /locus_tag="ISB30_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HAP1991947.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLVQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDVCAHYTDALIREIELEAD
                     SVLHQSGPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            2625..3119
                     /gene="hutX"
                     /locus_tag="ISB30_004385"
     CDS             2625..3119
                     /gene="hutX"
                     /locus_tag="ISB30_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HAP1991948.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            3119..3742
                     /gene="chuY"
                     /locus_tag="ISB30_004386"
     CDS             3119..3742
                     /gene="chuY"
                     /locus_tag="ISB30_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HAP1991949.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            3827..4783
                     /locus_tag="ISB30_004387"
     CDS             3827..4783
                     /locus_tag="ISB30_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP1991950.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            4780..5550
                     /locus_tag="ISB30_004388"
     CDS             4780..5550
                     /locus_tag="ISB30_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HAP1991951.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(5602..6249)
                     /locus_tag="ISB30_004389"
     CDS             complement(5602..6249)
                     /locus_tag="ISB30_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP1991952.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(6313..6639)
                     /gene="hdeB"
                     /locus_tag="ISB30_004390"
     CDS             complement(6313..6639)
                     /gene="hdeB"
                     /locus_tag="ISB30_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAP1991953.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(6755..7087)
                     /gene="hdeA"
                     /locus_tag="ISB30_004391"
     CDS             complement(6755..7087)
                     /gene="hdeA"
                     /locus_tag="ISB30_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAP1991954.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
BASE COUNT         1789 a         1792 c         1722 g         1813 t
ORIGIN      
        1 ccattaacac aataaacagg gggttatgaa atctatccag catccagaaa accatcctgt
       61 gtttatggct atccccccac aacctcatca caaaacgttg tcgttaacag tgataatttt
      121 ctcttctctt tgctctgcaa gccgcgatat ctccgggttc tcagcttttt cccgcgctga
      181 aatttaatcg gtaacaagaa cattagctga cgaagcatct tgataaaaat caaaaataaa
      241 tacgcttgta acgccgtgcg tattgaatcg acggttgtat ttcatatata actcaaatga
      301 taatcatgat cattctcaat ttgataagag ttctcacatt atgccaagga tcatcaccag
      361 gccgtttctt ttttccccac taacgttatg tatttcagcc gtggtctctg ccgcgaagac
      421 catggtaaaa cgaaaaaaat tgtttacggc attactggct cttagctgga cttttagcgt
      481 aacggctgcc gaacgtatcg tggtcgcagg aggatcgctg acggagctaa tctacgcgat
      541 gggcgctggc aaacgcgtgg ttggtgtcga tgaaacgaca tcctatccac cagaaaccgc
      601 caaactgccg catattggtt actggaaaca actcagcagc gaaggcattt tgtcacttcg
      661 cccggatagc gtaattacct ggcaggatgc aggaccgcaa attgtgctcg accaactgcg
      721 ggcgcaaaaa gtcaacgtcg tcactctgcc gcgtgtacca gccacccttg agcagatgta
      781 cgccaacatt cgccaactgg caaaaacgtt acaggttcct gaacaaggcg aggcgctggt
      841 gacacagatc aaccaacgcc tggagcgagt acagcaaaac gtggcggcca aaaaagcccc
      901 ggttaaagcc atgtttattc tctccgcagg cggaagtgcg ccacaggttg ccggtaaagg
      961 cagcgtcgcg gacgccattc tttcgcttgc cggagcagaa aacgtcgcta cccaccagca
     1021 gtacaaaagc tacagcgcgg aatcgctgat tgcggctaac cctgaagtga ttgtcgtcac
     1081 ctcacaaatg gtcgatggcg atattaatcg gctacgttct attgccggaa ttacccacac
     1141 tgccgcatgg aaaaaccagc gcattatcac cgttgatcaa aacctgattc tgggaatggg
     1201 tccgcgtatt gctgatgtcg ttgagtcttt acaccagcag ctttggccgc aataaatgga
     1261 tttaaatcag acgaatgaac acaaataaca ccctggatct cacgccacat tttgctttag
     1321 acggtgatca gccctttaaa gacaggcgcg ccatgatgcc gtttcgtggg gccattccgg
     1381 ttgccaaaga gcaactggta cagacctggc aagagatgat caatcaaacg gtatcaccac
     1441 gtaagcgact ggtttaccta cacattcctt tttgtgcgac acactgcaca ttttgtggtt
     1501 tttatcagaa ccgttttaat gaagatgtgt gtgctcatta taccgacgcc ctgattcgtg
     1561 aaatagaact ggaagcagac agcgtattgc atcagtcggg ccccatccac gcggtctatt
     1621 tcggtggcgg tacgccttcc gcgctttcag cacacgattt ggccagaatc atcaccacat
     1681 tacgagaaaa gttgccactg gcccctgatt gtgaaatcac tatcgaaggc cgggtactga
     1741 attttgacgc tgagcgaatc gatgcttgcc ttgatgctgg agcaaaccgc ttctcaattg
     1801 gtattcagtc gtttaacagc aaaatccgca agaaaatggc ccgcacctcg gatggcccaa
     1861 ccgctattgc gtttatggaa agcctggtca aacgcgaccg tgccgcggtg gtctgtgacc
     1921 tgctgtttgg tctgccgggt caggatgcgc aaacctgggg agaagatctg gctattgccc
     1981 gcgatatcgg tctcgacggc gtcgatctct atgcgctcaa tgtcctgccc aatacaccgc
     2041 tgggcaaagc cgtggaaaat gggcgtacta ccgtgccctc tccggcagaa cgtcgcgatc
     2101 tttacctgca agggtgtgat tttatggacg atgccggttg gcgctgcatc agtaacagcc
     2161 actggggccg taccacacgc gaacgcaatc tctataacct gctgattaaa caaggtgccg
     2221 attgtctggc ctttggttcc ggagccggcg gttcgattaa tggttactcc tggatgaacg
     2281 aacgcaatct tcagacctgg catgaatccg tcgcagcagg caaaaaaccg ctgatgatga
     2341 tcatgcgtaa cgccgaacgt aacgcgcagt ggcgtcatac cttgcagtcg ggcattgaaa
     2401 cggcgcgtgt accgctggac gaacttacgc cacatgccga aaaactcgcg ccgttactgg
     2461 cccagtggca ccaaaaaggc ttaagccgcg atgcgtcaac gtgtctgcgg ttgaccaatg
     2521 aaggtcgttt ctgggcaagc aacattttgc agtctcttaa tgaactgatt caggtactta
     2581 atgcgccagc aattgcgctt gaaaaaccat aacaggagtg atttatgagc catgtctcgt
     2641 tacaggaatt tttgaaaacg gagccagacg gtacgctgga ggcgatcgcc gaacaataca
     2701 acaccacgct gctggaggta gtgaaaaatc tgccctctcc gactgttgtg tcaggtgata
     2761 aattcgatac tgtctgggat accgtctgcg aatggggcaa cgtcaccacg ttagtacata
     2821 ctaccgatgt aatactcgaa tttagcggcg aactgccttc gggtttccat cgccacggct
     2881 atttcaacct acgcggaaag cacggcatgt ccgggcatat caaagcagaa aactgcacgc
     2941 atattgcctt gatcgaacgt aaatttatgg gcatggacac cgcctctatt ctctttttca
     3001 ataaggaagg cagcgcaatg ctgaaaatct tcctcggtag ggatgatcat cgtcagctcc
     3061 tgagcgaaca agtcagcgcc ttccatgctc tggcagcatc gttaaaggaa cacgcctgat
     3121 gacaccgtgg ctactctttg gtgcgggcgg aaaaggtgtg ggtgccagaa cgcttgaact
     3181 ggcgctggcg gaacaacgtc cggtcgttgc tgtcattcgt catgccgatg ccgccacgaa
     3241 actggcgcaa caaggcgtac aggtttttac aggcgacgcc tgtgatgcca gcgtagtcgc
     3301 ggctgcttgc cgcgcagctg gcccggatgc gcttatcatc tcaacaatgg gcggagctca
     3361 ggattatctg gcgcacagaa cagtgattga tgaggccgag aaggctggca tcagtcgcat
     3421 gattctggtg acctctttgg gatgcggcga tagctggccg tttttatcag agcgggcaaa
     3481 ggctgcgttt ggtcaggcgg tacgggaaaa aacgctggcg gaaagctggt tgcaaaccag
     3541 ccagcttgat tatgccattc tccgccctgg cggtctgctt gatggtgcgg caacgggcaa
     3601 agcacaacgg atacaaaatc aggagtgtca tggttttgtt aaccgtgctg atgtcgccgc
     3661 acatatccat gaactggcaa atgcgccagc acttaaccag caggtctaca gtctgattga
     3721 accggacctg aaaccggcgt gatcccccgg cgcggcatgt ccgcgccgta atgtcgtaac
     3781 ggagttttgc gtgctcaagg attcactctc ttccgccagg gtctttatgg ggctatcact
     3841 attattgcta gctctggtgc tgtttggtgc cagtcaggga gcgttaaaga tcagttttga
     3901 tgcccttttt gatgaggaat accgcgatat ctggctcaat attcgtctac cacgggtttt
     3961 gctggcggtg ctggtaggtg cagcgttggc aaccgcaggg gtaattatgc aagggctttt
     4021 tcgcaaccca atggctgacc ctggattact tggcgtcagt agcggttctg cattaatggt
     4081 tggcgttgct attgtgctgc ccttctcctt cccagtggtg ctggtgctct atgagcaaat
     4141 ggtgtttgcc attgccggaa gtttagtggt ctgcaccatc atttttctca tcacgcagcg
     4201 ccatcgcgat ggcagcatga tgcaattatt actcgccggt atcgccatca atgccctgtg
     4261 cggcgcagcg atcggcatcc tgagctatat cggcgatgag cagcagctac gacaactcac
     4321 attgtggatg atgggcaatc ttggacaggc gcaatggccg acgttattgg ttgccagttc
     4381 attcatccta ccggccatta tcgcaacaac ttgtctcgcc ggaacgctga atttactgca
     4441 gctcggtgat gaagaagccc actacctcgg cgtgaacgtt aagcgtaaac gccagcaatt
     4501 actgttagtg agctcactgc tcgttggtgc tgccgtatcg gtaagcggta ttatcggctt
     4561 tattggcctg gtgatcccgc atctgattcg catgactacc ggggcaaatc accgctggct
     4621 aatcccttgt tccgccctcg ccggagcctg tttattgctg atggcagaca cgcttgcccg
     4681 cacgctggta cagccagcag aaatgcccgt gggattatta accagcctgc ttggtggccc
     4741 ttattttatg tggttgattc tgcgcaaccg gaggatcaca tgatcagcgc ccagaatctg
     4801 gtttatagcc ttcaggggcg gcgtctgact gataatgtct cgctgacttt tcccggtggg
     4861 gaaattgttg ccatcctcgg ccctaatggt gcgggaaaat ctacgctgct tcgccagcta
     4921 accggctatc ttcaacctga tagcggcgaa tgtcggttgt ttaataagcc attaaatgaa
     4981 tggtcgatta ctgaactggc gaaacaccgg gccgtgatgc gtcaaaatag ccacatggcg
     5041 tttccattca gcgtacaaga agttatccag atggggcggc atccgcatcg tactggtaat
     5101 caggataatg aaacagcaca aataatggcg ttatgtgact gtcaggcgtt agcaaaccgc
     5161 gactatcggc aattatccgg tggtgaacaa cagcgcgtgc aattagcgcg cctgctggtg
     5221 caactctggg aacctactcc ctcaccgaaa tggttatttc ttgatgaacc aacttcggcg
     5281 ctggatattc accatcagca gcacttgttt cgtttattac gccagttagt tcacgaacgg
     5341 cagttcaatg tttgctgcgt attgcacgat ctaaatcttg ccgcgcgtta tgcagaccgt
     5401 gttgtattaa tgcaaaaagg aaaggttatc gcaaatggta agccgcagga cgtattgaca
     5461 cagcaggcgc tgacaatgct ctacggcgcg gatattaccg tacttaaaga tcctgccaac
     5521 cattcaccac tgatcgtcct cgatcattaa aaccaacagg agtaccgcac tggtactcct
     5581 gcgaataaat aactatacag attagctaat tgaaacgcct ttcacccctg ccataccttt
     5641 taataatcgc aacagatctt ctatcgacgt cgtggcatga agctggatat caatggcgac
     5701 cacttcatga tcttcatttt cctgcaacga aactaaatcc gtttttattt tttgctgctt
     5761 aaaccagtcg agcattgata ccgtattacc gttcactaat gtcagctgta aatggtaatt
     5821 tttgttcatc aggcggaagg ttaattgatg gaagacttcc agcaccagca aggtcatcac
     5881 tgaaccataa atacccagtt cgtacatacc gctgccaata accataccaa tggcggcggt
     5941 cacccagata tccgctgccg tcgtcagacc tacaatattt tggttacgaa cgagaatgtt
     6001 acctgcaccg ataaacccga cgcccgtcac aacctgagca gcaatacggc tggggtctag
     6061 tccgacgtga cctaaagaca gcacgtcagc aaaaccatat ttcgaaacaa tcataaacag
     6121 ggcgcttccc atgccaatta atacatgtgt gcgtaaccct gctcctttgc cgcgcatttg
     6181 tctttccatg ccaatagcgc cacaggcaat taccgcaagt ataagtcgta taataaattc
     6241 tgctgtcata taaaaatgca aaaaggagca gcaagatggc tcaacttgct actccttttt
     6301 acttgtacct cattaattcg gcaagtcatt agatgcttga tttttgaagg tatacaaatt
     6361 tttctgcggg tttttcttgc agtattcgat cactttagga atttgagtga gatcggtttc
     6421 atttaaagta acggtatcgc cacctttata tactgtttct tcatgcagca tccaccacgc
     6481 aactggtgtc attgcttttg gattcagatc aataaattcc tggcaggtca tatctttagc
     6541 ggattcattg gctgccagcg cagattgtgc gttcaccagt gacaaagccg ctacagcgcc
     6601 cataaaaata aacgctttac ggagagatga aatgttcatt ttgtaaccca ttcaatatag
     6661 agattatata caaacaggag gaatagctgc ttttaaagaa gatattccgg cctgttcaat
     6721 atgcaaggaa atacgatgta acgcattccc ggaattacat atctttctta attttgtccc
     6781 attcgccttt aactttatct ttaaagctgg cttttttatc ctgagtacaa gcctgaacga
     6841 tagctggggt tacggttgca ataccctgaa catctaaaac cgcatcttct ggtttatctt
     6901 tgttgttcag tgcttcagca aaaccaactg cagttggctg gaaggattcg tccacagcca
     6961 ggaaatcttc acaggtccag gagttgaccg gttttttgtt atcagctgct ttttgtgcat
     7021 ccgctgcatt gctcactact ggcagaagaa gcagaccacc aagaataacg cctaatactt
     7081 ttttcatcgt aatatcctca actataaagt gaaaga
//
LOCUS       DACADF010000204         7070 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.206, whole genome shotgun sequence.
ACCESSION   DACADF010000204 DACADF010000000
VERSION     DACADF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.206"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            29..1144
                     /locus_tag="ISB30_004392"
     CDS             29..1144
                     /locus_tag="ISB30_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1991955.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGENQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLDFAIQYQGKNSNRSTKKQNGDGYALSVDYNINGFGIVGAYSKSDRTNDQV
                     ADGNGSNAELWSLAAKYDANNVYAAVMYGETRNMTPGSIDTGVADQEGNTIMRDQLIN
                     ETQNFEAVVQYQFDFGLRPSLGYVYSKGKDIKGVPGHRYVDADRVNYIEVGTWYYFNK
                     NMNVYTAYKFNMLDKDDAAITGAAADDQFAVGIVYQF"
     gene            1737..2588
                     /gene="hchA"
                     /locus_tag="ISB30_004393"
     CDS             1737..2588
                     /gene="hchA"
                     /locus_tag="ISB30_004393"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAP1991956.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(2696..4054)
                     /locus_tag="ISB30_004394"
     CDS             complement(2696..4054)
                     /locus_tag="ISB30_004394"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAP1991957.1"
                     /translation="MKRLSITVRLTLLFIFLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVVIILCSLLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(4054..4725)
                     /gene="hprR"
                     /locus_tag="ISB30_004395"
     CDS             complement(4054..4725)
                     /gene="hprR"
                     /locus_tag="ISB30_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAP1991958.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4858..5271
                     /gene="hiuH"
                     /locus_tag="ISB30_004396"
     CDS             4858..5271
                     /gene="hiuH"
                     /locus_tag="ISB30_004396"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAP1991959.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5380..6384
                     /gene="msrP"
                     /locus_tag="ISB30_004397"
     CDS             5380..6384
                     /gene="msrP"
                     /locus_tag="ISB30_004397"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HAP1991960.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6385..7020
                     /gene="msrQ"
                     /locus_tag="ISB30_004398"
     CDS             6385..7020
                     /gene="msrQ"
                     /locus_tag="ISB30_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAP1991961.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     TPIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
BASE COUNT         1972 a         1658 c         1505 g         1935 t
ORIGIN      
        1 gaagattata atcacaaggg aatacataat gaaaagaaaa gttctggcaa tgctggtccc
       61 ggcgttatta gttgctggcg cagcaaatgc ggctgaaatt tataataaaa atggcaataa
      121 agttgatttt tacggcaaga tggttggcga acgcatctgg tcaaatactg atgataataa
      181 cagcgaaaac gaagatacct cctatgctcg tttcggtgtt aaaggtgaaa cccaaatcac
      241 cagcgaactg actggtttcg gtcagtttga atacaacctc gacgccagca agcctgaagg
      301 tgaaaaccaa gaaaaaaccc gtttaacctt cgccggtttg aaatataacg agttaggatc
      361 atttgactat ggccgtaact acggtgttgc ttatgatgcc gcagcttata ccgatatgtt
      421 agttgaatgg ggtggcgatt cctgggcttc cgctgacaac ttcatgaacg gtcgtaccaa
      481 cggtgttgca acctaccgta actctgattt ctttggcctg gttgatggtc ttgattttgc
      541 gattcagtac caaggcaaaa acagcaaccg tagcactaag aaacaaaatg gtgacggcta
      601 tgcgttgtct gtagactata acatcaatgg ttttggtatc gttggtgcat atagcaaatc
      661 tgatcgcacc aacgatcaag ttgccgatgg aaacggttca aatgctgaac tgtggtcatt
      721 ggctgcaaag tatgacgcga acaatgtcta cgcagctgta atgtacggtg aaactcgtaa
      781 tatgactccg ggttctattg atacaggtgt tgccgatcaa gaaggcaata caataatgcg
      841 tgatcaactt atcaatgaaa cccagaactt tgaagcggtt gttcagtacc aattcgactt
      901 tggtctgcgt ccgtccttag gttatgtgta ctctaaaggt aaagatatca aaggtgtacc
      961 tggacataga tatgttgacg ctgaccgtgt gaactacatt gaagttggta cctggtacta
     1021 cttcaataag aacatgaacg tttatactgc atacaaattc aacatgctgg ataaagatga
     1081 cgccgccatt actggcgcag cagctgatga ccagtttgca gttggtatcg tctaccagtt
     1141 ctaatcagcc aacgcgccga ggcgtgttac tgtaaaacat aacaccagcc tgagttcgtc
     1201 cctcatcgaa ctcaggcttt tttattggca tttcatctcc atctgcggca cacgcaattg
     1261 ccaaagcata tcgatacagc acattacccc gcccctcctg gctcaataaa atctaccagc
     1321 gtctcttcca gccaacaccc aaatgcattt ctttatgaat taatccgcaa caaaaccgct
     1381 tcccatatag aaatatttgc aaaaatagtg catgaaaaaa actttttccc taccaatccg
     1441 ggatataaat attcatcagt accacaacat tccacagcac taaatctctc cccatcgccc
     1501 ccaaacctct gataatggtc taaaatcatt gaggccactt gcgacgacgt tactcaataa
     1561 ggaaagtttg gcgtgaataa tattcaacac cagtgcagca tcatcattac atacttatgc
     1621 ccgccaacta ccgctttcga tatattgact caatttttca gcctggttaa aaatagcgac
     1681 cagctcagtc gcaaatatag tgactaccct aactaagcaa caataaggaa tacactatga
     1741 ctgttcaaac aagtaaaaac ccgcaggtcg atattgctga agataatgcg ttcttccctt
     1801 cagaatattc gcttagccaa tatacgagtc ccgtctctga tcttgatggc gtggactatc
     1861 caaaaccgta tcgcggtaaa cataaaattc tggtgatcgc cgcggacgaa cgttatttgc
     1921 cgaccgataa cggaaaactg ttctcgaccg gtaaccatcc gattgaaacg ttgctgccgt
     1981 tgtatcatct ccatgctgca ggtttcgaat tcgaagtggc gaccatttcc ggtctgatga
     2041 ccaagtttga atactgggct atgccgcaca aagatgaaaa agtaatgcca ttctttgaac
     2101 agcataaatc gttattccgt aatccgaaga aactcgcgga tgttgtcgcc agcctcaacg
     2161 ctgatagcga atatgcagca atctttgttc ctggtgggca tggcgcactt attggtttac
     2221 cagaaagcca ggacgtggct gccgctttac agtgggcaat caaaaatgac cgttttgtta
     2281 tctccctttg ccacggtccg gcagcttttc tggcacttcg ccacagcgat aacccactga
     2341 atggttattc tatttgcgca ttcccagacg ccgcagacaa acaaacgcca gatattggct
     2401 atatgccggg tcatctcacc tggtacttcg gcgaagagtt gaagaaaatg ggcatgaaca
     2461 tcattaatga cgacatcacc gggcgagtac ataaggaccg taaacttctc accggcgaca
     2521 gtccttttgc agcgaatgcg ttgggtaaac tggcggcgca ggaaatgctg gcagcttacg
     2581 cgagttaatc cctgttacat tgtaatgtaa gcgcataatc aatcgcagcc acgttaatac
     2641 tcacgacgtg gctgcgtttt ttcacggtta acttatggcg tactgaagcc cgatgttaat
     2701 ttctttgcgg taacgtaatt cggaacacgt tatgcttact gagatagtga tacgtagcac
     2761 ttcctccatg taattcggca atcgctttga ctaaagaaag gccaagtccc tgacctacgg
     2821 aatgacgcga attatctccc cgccaaaatc tacggaagag tttttcaggc tcattaattt
     2881 tcgttccagg actggcgata tcaatattaa gagaaccgtt ggcatcaaga aaactggtta
     2941 tatgaatacg cgatttttct ggtgaatatc gaatggcgtt aacaataaga ttcgataaca
     3001 ttcgttgcag taaaatttta tccgcaaaga tttgttgatt gcacttgacc ttaaagcaaa
     3061 tctctttctc gtctgaaaga tattcaagat agtccaaaag attttcaact tccttattga
     3121 gagaaagcga atccagtttc accaaaacat tgtttttgtc tgccctggca agaaacagta
     3181 tattctctgt taaccgcgaa atattttcca gctcttcgat gtttccggca attgtttttt
     3241 gatattcagc gatacttctg gtttgactga gcgtaacctg attctgaccc agtaatgcat
     3301 taattggcgt tctaagttca tgagcgagat cgtcagcaaa ttgactaagg cgctcaaaat
     3361 ctttgactaa agcatgatgc attttattca acgcctgccc aagaggtttt agttctctcg
     3421 gtaatgcgtt aacctcaacc ggcactcggc tatcgttata attcagcgct tccgttacgc
     3481 cactcaactt tttgatctct cgcaaccccg ttctgattaa caacggactt aataatgagc
     3541 acaatataat gacgacaatg caaattataa tgctattaat tttatactgt tcaagcatat
     3601 tatgtctggc tgaagccaat ttagccacag taaccgttaa tgatggcgaa acttcatcaa
     3661 tgttaattcg taaagcatct atctctgtat cattaataat gcttctgtaa atgcctgctg
     3721 cgctgattgt ctcactagca ggtatgttat ttaacatgtc atcactgaca tttgtccggt
     3781 taacaatttt attgatgcca tcaccatgaa tgatcaagat atcctgacta acatccatca
     3841 tccggttaaa atagacaggt aatgtatctg gatttacccc atcaattaac aactgcttga
     3901 tctgcgctgt ccggttaatg agtgttgtat catcgcgcca tttcaactca cttgccaggc
     3961 cattatagag agtccataca attccagcgc cagcaacaga cagcagaaat ataaaaagca
     4021 aggttaaacg gacggttata gataatcttt tcattatttt tttaccgcta cgaatgaata
     4081 gcccatcccc cggattgtgg cgattagctt ttcaggaaaa ggatcatcaa cttttgcgcg
     4141 gagcctgcga atggcgacat ccaccgtatt ggtatcacta tcaaagttga ttccccaaat
     4201 ttcactcgca ataaccgttc tgggtataat ctcgccagct ctggaggcca gtagccaaag
     4261 taactgaaac tccttgcgcg tcagtgtaat actgatattg tccctgctca cactttgact
     4321 gacagagtcc atttttaagc cactgatttc taatgttgaa ttcaaagcgt gatgttgcct
     4381 taattgtgcc cgaacccttg ccagcaactc agaaaatgaa aaaggtttta ccagataatc
     4441 atttgctcca ctgtccagcc ctctgactct gtcatcgaca gaatcccttg ctgtaaggca
     4501 aataacagga gtttgctttg ccgttcttaa cgtttgtaag atctgccagc catccatacc
     4561 cggaagcata atatccagaa tgatcaatgc ataatcatcc ttcagcgcaa gataaagccc
     4621 atctctgcca tcagaaacgg catcaatgac ataacccgct tcggaaagcc cctgcgttac
     4681 ccattcctgg gtcctttgat tatcttcaat aagtagaatc ttcatgaaaa taatatgcca
     4741 taaatcatat gttatagaaa caacctggtt cattacaaaa ttgtaatgct gctgtaaggt
     4801 taccctggac gttttttcgc tatcctcaaa actcattcac atgacaagga tataaacatg
     4861 ttaaagcgtt atttagtact ctcggtagca acggcagcat tttcattacc ttctttggtt
     4921 tatgccgcac aacaaaacat tcttagcgtg cacattttga accagcaaac aggaaaaccc
     4981 gctgccgacg tgacagtcac tcttgaaaag aaagcggata acggctggtt acaacttaat
     5041 accgccaaaa cagataagga tggacgaatt aaggcactgt ggcccgagca aactgcgact
     5101 actggcgatt accgtgtcgt atttaaaacc ggggactatt tcaagaaaca aaatcttgaa
     5161 agtttcttcc ctgagatccc cgttgaattt catattaata aagtgaacga gcattatcat
     5221 gtgcctttgt tactcagcca atatgggtat tcaacctatc gtggcagtta atgcgtaaat
     5281 atgtaaaagc tgatctctgc tactgttagg gaggtgactc cctgacagat taacagtaaa
     5341 cggctcttgt tggctaacga caaaaaagtg tgatggctta tgaaaaagaa tcaattttta
     5401 aaagaatcag atgttacggc cgagtcggta ttctttatga agcgtcggca ggtgttaaaa
     5461 gcactgggca tcagcgcagc tgcattttct ttgcctcacg ctgcgcatgc cgatctgctt
     5521 agctggttta aagggaacga tcgtccgccc gcccccgccg gaaaaccgct ggagttcagc
     5581 aagcctgccg cctggcaaaa taacctgcca ctgacgccag cagataaagt ctccggttat
     5641 aacaacttct atgaattcgg gctggataaa gccgatcccg ctgctaatgc tggtagcctg
     5701 aaaaccgatc catggacact gaaaatcagc ggcgaagtgg caaaaccatt gaccctcgat
     5761 catgatgatt taacccgtcg cttcccgctg gaagagcgta tttatcgtat gcgctgcgtg
     5821 gaagcgtggt cgatggtggt gccgtggatt ggttttccgc tacacaaatt gctggcgctt
     5881 gccgaaccca ccagcaatgc gaaatatgtc gctttcgaaa cgatttatgc gccggaacag
     5941 atgccaggcc agcaggaccg ctttatcggc ggcgggctga aatatcctta tgtcgaagga
     6001 ttgcgtctcg acgaagcaat gcatccgctc acactgatga ccgtaggtgt ttatggcaag
     6061 gcgttaccac cgcaaaatgg cgcgccggtg cgactgattg tgccgtggaa atatggcttt
     6121 aaagggatta aatcgatcgt cagtattaag ctgacccgcg agcgtccgcc aaccacctgg
     6181 aatctggcag cgcctgacga atacggtttt tacgccaacg ttaatccgca tgttgatcac
     6241 ccgcgctggt cacaggctac cgaacgattt attggttcag gcggcattct cgatgttcag
     6301 cgccagccaa cactactgtt taatggttac gccgaccagg tggcatcgct gtatcgtggc
     6361 ctggatttgc gggagaattt ctaaatgcgc ctgacagcaa aacaggtgat atggctgaaa
     6421 gtttgcctgc atcttgccgg attgttgccg tttctctggc tggtctgggc gatcaatcac
     6481 ggtggactag gtgccgatcc ggtgaaagat attcagcatt ttaccggtcg cactgcactg
     6541 aaatttttgc ttgcggcctt gttaatcaca cctctggcac gctacgcaaa acagccgtta
     6601 ttgatacgca ctcgccgcct gttaggctta tggtgctttg cctgggcgac actgcattta
     6661 accagttacg cattgctgga gttgggcgtg aataatctgg cgttattagg taaggagtta
     6721 attacccggc cttatttaac gttaggaatt atcagctggg ttattttgct tgctttagcg
     6781 ttcacttcaa cccagtcgat gcagcgaaaa ctcggcaaac actggcaaca gttgcataac
     6841 ttcgtctatc ttgtcgcgat cctgacccca atacattatc tgtggtcagt gaagattatt
     6901 tcaccgcagc cactcatcta cgctgggctg gctgtactgc ttttagcctt acggtataag
     6961 aagttgcttt cccttttcaa ccggttacgc aagcaggccc acaacaaatt atctctataa
     7021 cttcatattg ccgacaaact tcgcgttatc ccggccggat gcggcgcgag
//
LOCUS       DACADF010000205         6908 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.207, whole genome shotgun sequence.
ACCESSION   DACADF010000205 DACADF010000000
VERSION     DACADF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.207"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            469..1458
                     /locus_tag="ISB30_004399"
     CDS             469..1458
                     /locus_tag="ISB30_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1991962.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1528..3042
                     /locus_tag="ISB30_004400"
     CDS             1528..3042
                     /locus_tag="ISB30_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAP1991963.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTNMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3054..4043
                     /locus_tag="ISB30_004401"
     CDS             3054..4043
                     /locus_tag="ISB30_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAP1991964.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPV
                     VRTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGK
                     ISYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4210..5010
                     /gene="otsB"
                     /locus_tag="ISB30_004402"
     CDS             4210..5010
                     /gene="otsB"
                     /locus_tag="ISB30_004402"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HAP1991965.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAITAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4985..6409
                     /gene="otsA"
                     /locus_tag="ISB30_004403"
     CDS             4985..6409
                     /gene="otsA"
                     /locus_tag="ISB30_004403"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HAP1991966.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6416..6844)
                     /gene="uspC"
                     /locus_tag="ISB30_004404"
     CDS             complement(6416..6844)
                     /gene="uspC"
                     /locus_tag="ISB30_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HAP1991967.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGG"
BASE COUNT         1720 a         1622 c         1815 g         1751 t
ORIGIN      
        1 atggctctca ttatacgtgt tctgttaatt aatgagcaca gtgataatta atggcgaaat
       61 gaatgacgcg cattttccac atctttaagt tgcggttatt acaccattgc aaaaactcaa
      121 cgccaggtaa tgaggcctat tgactggtta aaaagaagac atcccgcatg ggtaccaaag
      181 acaacaagga cttccaggct aatcttatgg attattctgc tgtgcactcg ataatttgtc
      241 tgacaaattc gcttttcctt atgtcttttc ccgctaaatt tatgcacgtt ctcactgtaa
      301 ttctgcgatg tgatattgct ctcctatgga gaattaattt ctcgctaaaa ctatgtcaac
      361 acagtcactt atcttttagt taaaaggtaa tgctttgttt tccgattaat ttaacgaatg
      421 tcattcgttt ttgccctaca caaaacgaca ctaaagctgg agagaaccat gcacaaattt
      481 actaaagccc tggcagccat tggtctggca gccgttatgt cacaatccgc tatggcggag
      541 aacctgaagc tcggtttcct ggtgaagcaa ccggaagagc cgtggttcca gaccgaatgg
      601 aagtttgccg ataaagccgg gaaggattta gggtttgaag ttattaagat tgccgtgccg
      661 gatggcgaaa aaacattgaa cgctatcgac agcctggccg ccagtggcgc gaaaggtttc
      721 gttatttgta ctccggaccc gaaactcggc tctgccatcg tggcgaaagc gcgtggctac
      781 gatatgaaag tcattgccgt ggatgaccag tttgttaacg ccaaaggtaa gccaatggat
      841 accgttccgt tggtgatgat ggctgcgacc aaaattggcg aacgtcaggg ccaggaactg
      901 tataaagaga tgcagaaacg tggctgggat gtcaaagaaa gcgcggtgat ggcgattacc
      961 tccaacgaac tggatacggc tcgtcgccgt actacggggt ctatggatgc gctgaaagct
     1021 gcaggattcc cggaaaaaca aatttatcag gtaccgacca aatctaacga catcccgggg
     1081 gcatttgacg ctgccaactc aatgctggtt caacatccgg aagttaaaca ttggctgatt
     1141 gtcggtatga acgacagcac cgtgctgggc ggcgtacgcg cgacggaagg tcagggcttt
     1201 aaagcggccg atatcatcgg tatcggtatt aacggtgtgg atgcggtgag tgaactgtct
     1261 aaggcgcagg caaccggctt ctacggttcc ctgctgccaa gcccggacgt acatggttat
     1321 aagtccagcg aaatgcttta caactgggtt gcaaaaggtg ttgaaccgac caaattcacc
     1381 gaagttaccg acgtggtgct gatcacgcgt gacaacttta aagaagaact ggagaaaaaa
     1441 ggtttaggcg gtaagtaatt tgccggaaaa attcccctct gcatgatgca gagggggtgt
     1501 gaacgaccag tgattcacgg agacgttatg caacagtcta ccccgtatct ctcatttcgc
     1561 ggcatcggta aaacgtttcc cggcgttaag gcgctgacgg atattagttt tgactgctat
     1621 gccggtcagg ttcatgcgtt gatgggtgaa aatggcgcag gaaaatcaac tctcttaaaa
     1681 atcctcagcg gaaactatgc gccaaccacg ggttctgtag tgattaatgg gcaggaaatg
     1741 tccttttccg acacgaccgc agcacttaac gcgggtgtgg cgattattta tcaggaactg
     1801 catctcgtgc cggaaatgac cgtcgcggaa aatatctatc tcggccagct gccgcataaa
     1861 ggcggcattg tgaatcgttc attgctgaat tatgaggcag gtttacaact taaacatctt
     1921 ggtatggata ttgacccgga cacgccgctg aaatatctct ccattggtca gtggcagatg
     1981 gttgaaatcg ccaaagcgct ggcgcgtaac gccaaaatta tcgcctttga tgagccaacc
     2041 agctccctct ctgcccgcga aatcgacaat cttttccgcg ttattcgtga attgcgaaaa
     2101 gaggggcggg tgatcttata cgtttctcac cgtatggaag aaatatttgc cctcagcgat
     2161 gccatcaccg tctttaaaga tgggcgttat gtcaaaactt ttaccaatat gcagcaggtt
     2221 gaccacgacg cactggtgca ggcgatggtc gggcgagaca ttggcgatat ctacggctgg
     2281 caaccgcgta gttatggcga ggagcggctg cgtcttgatg ctgtgaaagc accaggcgtg
     2341 cgtacgccaa taagtctggc ggttcgcagt ggagaaattg tcggtctgtt tggtctggtc
     2401 ggggcagggc gtagcgaatt aatgaaaggc atgtttggcg ggacgcaaat caccgccggt
     2461 caggtttata tcgaccaaca gccgatcgat attcgtaaac cgagccacgc cattgccgca
     2521 ggcatgatgc tctgcccgga agatcgcaaa gcggaaggca ttattcccgt gcactccgtt
     2581 cgcgacaata tcaacatcag tgccagacgt aaacatgtgc ttggcggttg tgtaatcaac
     2641 aacggttggg aagaaaacaa tgccgatcac cacattcgtt cgctcaacat caaaacgccg
     2701 ggcgctgagc aactgatcat gaatctctca ggcggaaatc agcaaaaagc cattctgggc
     2761 cgctggttat cggaagagat gaaggtcatt ttgctggatg aacctacgcg tggcattgat
     2821 gttggtgcta agcatgaaat ttacaacgtg atttatgcgc tggcggcgca gggtgtggcg
     2881 gtgctgtttg cctccagcga cttacctgaa gtcctcggcg ttgccgaccg gattgtggtg
     2941 atgcgggaag gcgaaatcgc cggtgaattg ttacacgagc aggcagatga gcgtcaggca
     3001 ctgagccttg cgatgcctaa agtcagccag gcagttgcct gagtaaggag agtatgatgt
     3061 cttctgtttc tacatcgggt tctggcgcac ctaagtcgtc attcagcttc gggcgtatct
     3121 gggatcagta cggcatgctg gtggtgtttg cggtgctttt tatcgcctgt gccatttttg
     3181 tcccaaattt tgccaccttc attaatatga aagggttggg cctggcaatt tccatgtcgg
     3241 ggatggtggc ttgtggcatg ttgttctgcc ttgcttccgg tgactttgac ctttctgtcg
     3301 cctccgtaat tgcctgtgcg ggtgtcacca cggcggtggt tattaatctg actgaaagcc
     3361 tgtggattgg cgtggcagcg gggttgctgc tgggcattct ctgtggtctg gtcaatggct
     3421 ttgttatcgc caaactcaaa ataaatgccc tgatcacgac actggcaacg atgcagattg
     3481 ttcgaggtct ggcgtacatc atttcagacg gtaaagcggt cggtatcgaa gatgaaagct
     3541 tctttgccct tggttacgcc aactggttcg gtctgcctgc gccaatctgg ctcaccgtcg
     3601 cgtgtttgat catctttggc ttgctgctga ataaaaccac ctttggtcgt aataccctgg
     3661 cgattggcgg gaacgaagag gctgcgcgtc tggcgggtgt accggttgtt cgcaccaaaa
     3721 ttattatctt tgttctctct ggcctggtat cagcgatagc cggaattatt ctggcttcac
     3781 gtatgaccag tgggcagcca atgacgtcga ttggttatga gctgattgtg atctccgcct
     3841 gcgttttagg tggcgtttct ctgaaaggcg gcatcggaaa aatctcatat gtggtggcgg
     3901 gtatcttaat tttaggcacc gtggagaacg ccatgaacct gcttaatatt tctcctttcg
     3961 cgcagtacgt ggttcgcggc ttaatcctgc tggcagcggt gatcttcgac cgttacaagc
     4021 aaaaagcgaa acgcactgtc tgatgctttt ttctgcaaca atttagcgct tttttcctcc
     4081 acagccagcc gccacaacgg ttggctgttc ttcgttacaa atggcgaccc ccgtcacact
     4141 gtctatactt acatgtctgt aaagcgcgtt ctgcgcaaca caataagaaa agagaaggag
     4201 gagaaccggg tgacagaacc gttaaccgaa acccctgaac tatccgcgaa atatgcctgg
     4261 ttttttgatc tcgatggaac gctggcggaa atcaaaccgc atcccgatca ggtcgtcgtg
     4321 cctgacacta ttctgcaagg acttcagcta ctggcaaccg caagtgatgg tgcattggca
     4381 ttgatttcag ggcgctcaat ggtggagctt gacgcactgg caaaacctta tcgcttccca
     4441 ttagcgggcg tgcatggggc ggagcgccgt gacatcaatg gtaaaacgca tatcgttcat
     4501 ctgccggatg cgattgcgcg tgatattagc gtgcaactgc atacggtcat cgcccagtat
     4561 cccggcgcgg agctggaggc gaaagggatg gcttttgcgc tgcattatcg ccaggctccg
     4621 cagcatgaag acgcattaat gacattagcg caacgtatta ctcagatctg gccacaaatg
     4681 gcgttacagc agggaaagtg tgttgtcgag atcaaaccga aaggtaccag taaaggtgag
     4741 gcgattactg cctttatgca ggaagctccc tttatcgggc gaacgcccgt atttctgggc
     4801 gatgatctaa ccgatgaatc tggcttcgca gtcgttaacc ggctgggcgg aatgtcagta
     4861 aaaattggca caggtgcaac tcaggcatca tggcgactgg cgggcgtgcc ggatgtctgg
     4921 agctggcttg aaatgataac caccgcatta caacaaaaaa gagaaaataa caggagtgat
     4981 gactatgagt cgtttagtcg tagtatctaa ccggattgca ccaccagacg agcacgccgc
     5041 cagtgccggt ggccttgccg ttggcatact gggggcactg aaagccgcag gtggactgtg
     5101 gtttggctgg agcggtgaaa cagggaatga ggatcagccg ctaaaaaagg tgaaaaaggg
     5161 taacattacg tgggcctctt ttaacctcag cgaacaggac cttgacgaat actacaacaa
     5221 attctccaat gccgttctct ggcccgcttt tcattatcgg ctcgatctgg tgcaatttca
     5281 gcgtcctgcc tgggatggct atctacgcgt aaatgcgttg ctggcagata aattactgcc
     5341 gctgttgcaa gacgatgaca ttatctggat ccacgattat cacctgttgc cctttgcgca
     5401 tgaattacgc aaacggggag tgaataatcg cattggtttc tttctgcata ttcctttccc
     5461 gacgccggaa atcttcaacg cgctgccgac atatgacacc ttgcttgaac agctttgtga
     5521 ttatgatttg ctgggtttcc agacggagaa cgatcgtctg gcgttcctgg attgcctttc
     5581 taacctgacc cgcgtcacga cacgtagcgc aaaaagccat acggcctgcg gcaaagcgtt
     5641 tcgaacggaa gtttacccga tcggcattga gccgaaagaa atagcgaaac aggctgccgg
     5701 tccactgccg ccaaaactgg cgcaactcaa agcagaactg aaaaacgtac aaaatatctt
     5761 ttctgtcgaa cgactggatt attccaaagg tttgccggag cgttttctcg cctatgaagc
     5821 gttactggaa aaatatccgc aacatcacgg taaaattcgt tatacccaga tcgcgccaac
     5881 gtctcgtggt gatgtgcaag cctatcagga tattcgtcat cagctcgaaa atgaagccgg
     5941 acgaattaat ggtaaatatg ggcaattagg ctggacgccg ctttattatt tgaatcagca
     6001 ttttgaccgt aaattactga tgaaaatatt ccgctactct gacgtgggct tagtgacgcc
     6061 actgcgtgac gggatgaacc tggtggcaaa agagtatgtt gccgctcagg acccagccaa
     6121 cccgggcgtt cttgttcttt cgcaatttgc gggagcggca aacgagttaa cgtcggcgtt
     6181 aattgttaat ccctacgatc gtgacgaagt tgcagctgcg ctggatcgtg cattgactat
     6241 gtcgctggcg gaacgtattt cccgtcatgc agaaatgctg gacgttatcg tgaaaaacga
     6301 tattaaccac tggcaggagt gcttcattag cgacctaaag cagatagttc cgcgaagcgc
     6361 ggaaagccag cagcgcgata aagttgctac ctttcctaag cttgcgtagg tgcggttaac
     6421 ctcccgtaag tggaactaac agcacatcga cctcacttga ggtaatcact cttttcgccg
     6481 agcaggatgc tcgcgaaaag aaactgtgat tgtgattacc gcaaatcacc aaatcgaaat
     6541 gatgtttgcg acaaacttcc agaatatgtt cgcttaactc accgtaggca ataaacgtct
     6601 tgtcaacggg ataccccgca tcctgaatta acttatcaag aaagctttgc gtttcttcct
     6661 gcatcacgtt gcgtaaatct tcaagcatcg gcgcagctaa ttgattgtac atttccgggt
     6721 cggaagcgag agtaattaaa ctgatgtgtc cctttactgg cctggcgata gataccgctt
     6781 ttgccagcag ttgctgactt tccggtgtaa ccgcaacggc gacaagaata ttgctatagc
     6841 tcataacctg ttccttattc tgtgaacttc aggtgacatt aaagctttac tcctaatgga
     6901 gaaacgac
//
LOCUS       DACADF010000206         6885 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.208, whole genome shotgun sequence.
ACCESSION   DACADF010000206 DACADF010000000
VERSION     DACADF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.208"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            99..1496
                     /gene="ulaA"
                     /locus_tag="ISB30_004405"
     CDS             99..1496
                     /gene="ulaA"
                     /locus_tag="ISB30_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP1991968.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFASRAL
                     RAEEDAEKQLAEQSA"
     gene            1512..1817
                     /gene="ulaB"
                     /locus_tag="ISB30_004406"
     CDS             1512..1817
                     /gene="ulaB"
                     /locus_tag="ISB30_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAP1991969.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1827..2291
                     /gene="ulaC"
                     /locus_tag="ISB30_004407"
     CDS             1827..2291
                     /gene="ulaC"
                     /locus_tag="ISB30_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAP1991970.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2305..2955
                     /gene="ulaD"
                     /locus_tag="ISB30_004408"
     CDS             2305..2955
                     /gene="ulaD"
                     /locus_tag="ISB30_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP1991971.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2965..3819
                     /locus_tag="ISB30_004409"
     CDS             2965..3819
                     /locus_tag="ISB30_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP1991972.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMT
                     KAGMVEAV"
     gene            3819..4505
                     /locus_tag="ISB30_004410"
     CDS             3819..4505
                     /locus_tag="ISB30_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP1991973.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFVWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4634..4909)
                     /locus_tag="ISB30_004411"
     CDS             complement(4634..4909)
                     /locus_tag="ISB30_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1991974.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5237..5632
                     /gene="rpsF"
                     /locus_tag="ISB30_004412"
     CDS             5237..5632
                     /gene="rpsF"
                     /locus_tag="ISB30_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP1991975.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5639..5953
                     /gene="priB"
                     /locus_tag="ISB30_004413"
     CDS             5639..5953
                     /gene="priB"
                     /locus_tag="ISB30_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAP1991976.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5958..6185
                     /gene="rpsR"
                     /locus_tag="ISB30_004414"
     CDS             5958..6185
                     /gene="rpsR"
                     /locus_tag="ISB30_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP1991977.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6227..6676
                     /gene="rplI"
                     /locus_tag="ISB30_004415"
     CDS             6227..6676
                     /gene="rplI"
                     /locus_tag="ISB30_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP1991978.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         1648 a         1709 c         1886 g         1642 t
ORIGIN      
        1 ataaataatc ttgttgtgat tacttttgaa aattagagtg agagcacaac attccgggtg
       61 tgcgaaatac ccggttacct cttcttcagg agatcgttat ggagatcctc tacaacatct
      121 ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt gttgctgggt attgtgacct
      181 gtctgggtta catcctactg cgcaaaagtg tcagcgttat tattaaaggt acgattaaaa
      241 ccataattgg tttcatgttg ttgcaggcag ggtccggcat cctgaccagc accttcaaac
      301 cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg cgcaatttcc gatacttacg
      361 cgtcgatgat ggcaaccatt gaacgcatgg gcgatgccta tagctgggtg ggttacgcgg
      421 tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt accggtattc
      481 gaaccatcat gctgactggt cacatcatgt tccagcaggc ggggctgatt gccgttacgc
      541 tgtttatctt cggctactcc atgtggacga ccattatctg caccgcgatt ctggtttcgc
      601 tctactgggg cattacctcc aacatgatgt acaagccgac acaggaagtc accgacggct
      661 gtggtttctc cattggtcac cagcagcagt tcgcatcatg gattgcttat aaagtcgcac
      721 cgttccttgg caaaaaagag gaaagcgttg aagacctcaa actgcctggc tggctgaata
      781 ttttccacga caacatcgtc tccacggcga ttgtcatgac tatcttcttt ggtgccattc
      841 tgctctcctt tggtatcgac accgtgcagg cgatggcagg caaagtgaac tggacggtgt
      901 acatcctgca aaccggtttc tcctttgcgg tggcgatctt catcatcact cagggcgtgc
      961 gcatgtttgt ggcggaactc tctgaagcat ttaacggtat ctcccagcgt ctgatcccag
     1021 gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgcgccgaac gccgtggtct
     1081 ggggttttat gtggggcacc atcgggcaac tgattgcggt tggtatcctg gtcgcctgtg
     1141 gctcttcaat cctgattatt cccggcttta tcccgatgtt cttctccaac gccactatcg
     1201 gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct gaaaatttgt ctggtgatgg
     1261 ggatgatcga aatctttggt tgcgtctggg cagtgaaact caccggtatg agtgcctgga
     1321 tgggtatggc ggactggtcg attctggcac cgccgatgat gcagggcttc ttctccatcg
     1381 gtatcgcctt tatggccgtc atcattgtaa ttgcactggc ttatatgttc ttcgctagcc
     1441 gcgcgctgcg cgcagaagaa gatgcagaaa aacaactggc agaacaatct gcttaataag
     1501 gagttttgat tatgaccgtt cgtattctgg ctgtgtgtgg taacggacaa ggcagttcca
     1561 tgatcatgaa aatgaaagtg gaccagtttt taacccagtc gaacattgac cacacggtaa
     1621 acagctgcgc ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc atcatcgctt
     1681 ctacccatat tgcgggcgaa atcaccgtga ccggcaacaa atatgtggtt ggcgtgcgca
     1741 acatgctctc tcctgctgat tttggcccga aactgctgga agtgatcaaa gagcatttcc
     1801 cgcaggacgt gaagtaagga cgcgccatga aattacgtga ttcgctggct gaaaataaat
     1861 ctatccgcct gcaagcggaa gcagagacat ggcaggacgc agtgaaaatc ggcgttgatc
     1921 tgctggtagc ggcagatgtg gtagagccgc gttactacca ggcgattctg gatgcggttg
     1981 aacagcacgg cccgtacttt gtgctcgccc ccggcctggc aatgccgcac ggacgcccgg
     2041 aagagggcgt taagaaaacc ggttttgcgc tggtgacgct gaaaaagccg ctggagttca
     2101 accacgaaga taacgatccg gtggatatcc tcatcaccat ggcggcggtt gatgccaaca
     2161 ctcaccagga agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa gagaatttcg
     2221 accgtttacg tgcctgccgt accgagcagg aagtactgga tctcattgac cgcaccaacg
     2281 cggcagctta agaaggaatt gaacatgtca ttaccgatgt tgcaagtcgc gctggacaac
     2341 cagactatgg atagcgccta cgaaaccact cgcctgattg ccgaagaagt cgacattatc
     2401 gaagtgggca ccattctttg cgtaggcgaa ggcgtgcgtg cggttcgtga cctgaaagcg
     2461 ctgtacccgc acaaaatcgt actggctgac gccaaaattg ccgatgcagg caaaatcctt
     2521 tctcgcatgt gcttcgaagc caacgctgac tgggtgaccg taatttgttg tgcggatatc
     2581 aacaccgcca aaggcgcgct ggacgtggca aaagagttta atggcgacgt gcagatcgaa
     2641 ctgaccggct actggacctg ggaacaggcg cagcagtggc gcgacgcagg cattgggcag
     2701 gtggtttatc accgcagccg tgacgcgcag gccgcaggcg tggcgtgggg cgaagcggac
     2761 attaccgcga tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac cggtggtctg
     2821 gcgctggaag atctgccgtt gtttaaaggc attccgattc atgtgtttat cgctggtcgc
     2881 agcatccgtg atgcagcctc tccggttgaa gccgcgcgtc agttcaaacg ttccatcgcc
     2941 gaactgtggg gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg catctacgaa
     3001 aaagcgctcc ccgctgggga gtgctggctg gaacgactgc aactggcaaa aacgttaggc
     3061 ttcgattttg tcgagatgtc agtagatgaa acggacgatc gcctttcgcg cctcgactgg
     3121 agccgcgagc agcgtctggc actggtgaac gcgattgttg aaaccggcgt gcgcgtaccg
     3181 tccatgtgcc tttctgctca tcgtcgtttc ccgctgggaa gcgaagatga cgcggtgcgg
     3241 gcgcaggggc tggagattat gcgtaaagct atccagttcg cccaggacgt cggcattcgc
     3301 gtgatccaac tggcgggcta tgacgtttac tatcaggaag ccaataacga aacgcgtcgt
     3361 cgttttcgtg acggcctgaa agagagcgtt gagatggcaa gccgcgcgca ggtcacgctg
     3421 gcgatggaga tcatggatta cccattgatg aactccatca gcaaggcgct gggctacgca
     3481 cactatctca acaatccgtg gttccagctc tacccggata tcggcaacct gtcggcgtgg
     3541 gacaacgacg tgcagatgga gttgcaggcc ggaatcgggc atatcgtcgc ggtacatgtg
     3601 aaagacacca aaccaggcgt cttcaaaaac gtgccttttg gcgaaggtgt tgtggatttt
     3661 gaacgttgct tcgaaacgct caaacagagt ggctattgcg ggccgtacct gattgagatg
     3721 tggagcgaaa cggcggaaga tccggcggca gaagtggcga aagcgcgcga ttgggtgaaa
     3781 gcgcgcatga ccaaagcagg catggtggag gcggtataat gcaaaagctg aaacagcagg
     3841 tgtttgaggc caacatggat ctgccgcgtt acgggctggt gacctttacc tggggcaacg
     3901 tcagcgccat tgaccgcgaa cgcgggctgg tggtgatcaa gcccagcggc gtcgcctacg
     3961 aaaccatgaa agcagacgat atggtggtgg tcgatatgag cggtaaggtg gtggaagggg
     4021 agtatcgtcc gtcttccgac actgcgacgc atctcgaact ctaccgtcgt tatccgtcgc
     4081 ttggtggtat tgtccatacc cactccactc atgccaccgc atgggcgcag gcggggctgg
     4141 cgatcccggc gttaggcacc acgcacgccg attacttctt tggcgacatc ccgtgcacgc
     4201 gcggattaag cgaagaagag gtgcagggtg agtacgaact gaataccggc aaagtgatta
     4261 tcgaaacgct gggtgacgcc gagccgctgc atacgccggg aattgtggtg tatcagcacg
     4321 gaccgttcgt ctgggggaaa gatgcccacg atgcggtgca taacgcggtg gtgatggaag
     4381 aagtggcgaa aatggcgtgg attgcgcgca gtattaaccc acaactgaat cacatcgaca
     4441 gctttctgat gaataaacac ttcatgcgta agcacggtcc caacgcctat tacgggcaga
     4501 agtaaaacac gcgctgcgga aatttccttc ttcgggagat aactggtcta attccgcagc
     4561 cgtttttcaa aaaaagcccc ctgcgaaggg ggcaaagcaa actatggcaa tgtttcgttg
     4621 gttatacctg gtgctagcga taaatatccg cgctggcgtg catattgccg ttactccccg
     4681 gttcccgcat cagaatcaca tggtagtacg ttgcgccttg cgcatctgtc tcttcattta
     4741 atgcctgacg tgcttcactt tcagtggcga agttatggtt gatataaatc acacctaagc
     4801 tttgcacatc atccatattt ctggcctggt ggttattaat ttcaatggct gcccatgtat
     4861 ttgcacttag caaaagcaca gccagaaggg ctaaaacacg actgaacata gatacctcct
     4921 cgacggctga ctttgtgtgc tctcctcttg tggtgatctt ctgatttaat tttaatcaat
     4981 gataaagaag ttgatggtga ccatttctga tgcagttgtt caaaaaaaca ccatgacgaa
     5041 gtgtgatgaa ctccaaatca gcgatttaga ggttatttgc gaaaggggcg atttatttct
     5101 gctcagccct tgagtttagc ggggtatgtc agtactataa cgcgtcattt ttcagccgac
     5161 ctttaacacg ttccttgcct ccccgggatt cggctgaccc agacaggagg ctgaataatc
     5221 cgtaaggagc aattcgatgc gtcattacga aatcgttttt atggtccatc ctgaccagag
     5281 cgaacaggtt ccgggcatga tcgagcgcta cactgctgcc atcactggtg cagaaggcaa
     5341 gatccaccgt ctggaagact ggggccgccg tcagctggct tacccgatca acaaactgca
     5401 caaagcacac tacgttctga tgaacgttga agctccgcag gaagtgatcg atgagctgga
     5461 aactaccttc cgcttcaacg atgccgttat ccgcagcatg gttatgcgta ccaagcacgc
     5521 tgttaccgaa gcatctccga tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga
     5581 tttcgcaaac gaaaccgctg atgatgctga agctggggat tctgaagagt aatttctgat
     5641 gaccaaccgt ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc
     5701 atcaggaatt cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg
     5761 ctttcaccgg caggcgtggt gccaaatgcc cgttattgtt agcggacacg aaaaccaggc
     5821 cattactcac agtataacgg tcggcagtcg cataaccgtt caggggttca tttcatgcca
     5881 taaggcaaag aacggactga gcaaaatggt tttgcatgcc gagcagattg aattgataga
     5941 ttctggagac tagccatatg gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg
     6001 cggaaggcgt tcaagagatc gactataaag atatcgctac gctgaaaaac tacatcaccg
     6061 aaagcggtaa gattgtccca agccgtatca ccggtacccg tgcaaaatac cagcgtcagc
     6121 tggctcgcgc tatcaaacgc gctcgctacc tgtccctgct gccgtacact gatcgccatc
     6181 agtaatcggt cacggtccat taatacgact ttgagaggat aaggtaatgc aagttattct
     6241 gcttgataaa gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta aagcgggcta
     6301 tgctcgtaac ttcctggtac cgcagggtaa agctgttcca gctaccaaga aaaacattga
     6361 attcttcgaa gcacgtcgcg ctgaactgga agctaaactg gctgaagttc tggcagcagc
     6421 taatgctcgc gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg
     6481 cgacgaaggt aaactgttcg gttccatcgg tactcgcgac atcgctgacg ctgtaactgc
     6541 agctggcgtt gaagtggcta agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac
     6601 tggtgaacac gaagtgagct tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa
     6661 cgtagtagct gaataattcg ttattcaacg agacgtaaaa acgccgacca gtggtcggcg
     6721 ttttgctttt aagtgataaa aaccgcagaa acagacgttc cctccataaa tctcttgcta
     6781 ttccttttat aaaaagttcg cagtacccat acctggcccc cagcctcgcc gctggtagtt
     6841 cgtgtcatgt tttttacgcg taaaacacga caaaagcatc aatac
//
LOCUS       DACADF010000207         6865 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.209, whole genome shotgun sequence.
ACCESSION   DACADF010000207 DACADF010000000
VERSION     DACADF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.209"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            5..1714
                     /gene="adeD"
                     /locus_tag="ISB30_004416"
     CDS             5..1714
                     /gene="adeD"
                     /locus_tag="ISB30_004416"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP1991979.1"
                     /translation="MLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIAGVG
                     AEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICDPHE
                     IVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRDHPQ
                     VTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIAAGIENCHES
                     YQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIAHEG
                     HIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDARKVT
                     VQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQPVSASDFALQFTPGKRYRVI
                     EVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGLNEGALAA
                     TVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMSTDTA
                     QSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFTTLE
                     VTE"
     gene            complement(1761..3152)
                     /gene="uhpT"
                     /locus_tag="ISB30_004417"
     CDS             complement(1761..3152)
                     /gene="uhpT"
                     /locus_tag="ISB30_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAP1991980.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3290..4609)
                     /gene="uhpC"
                     /locus_tag="ISB30_004418"
     CDS             complement(3290..4609)
                     /gene="uhpC"
                     /locus_tag="ISB30_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAP1991981.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNSAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4619..6121)
                     /gene="uhpB"
                     /locus_tag="ISB30_004419"
     CDS             complement(4619..6121)
                     /gene="uhpB"
                     /locus_tag="ISB30_004419"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAP1991982.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLMQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6121..6711)
                     /gene="uhpA"
                     /locus_tag="ISB30_004420"
     CDS             complement(6121..6711)
                     /gene="uhpA"
                     /locus_tag="ISB30_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAP1991983.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
BASE COUNT         1731 a         1975 c         1795 g         1364 t
ORIGIN      
        1 ggaattgtta gccgtttccc gtggagacgc tgttgccgat tatattattg ataatgtctc
       61 tattctcgac ctgatcaatg gcggagaaat ttccggccca attgtgatta aaggacgtta
      121 cattgccggt gttggcgcag aatacgctga tgctccggct ttgcagcgga ttgatgcccg
      181 cggcgcaacg gcggtgccag ggtttattga tgctcacctg catattgaat ccagcatgat
      241 gacgccggtc acctttgaaa ctgctaccct gccgcgcggc ctgacgaccg ttatttgcga
      301 ccctcatgaa atcgtcaacg tgatgggaga agccggattc gcctggtttg cccgctgtgc
      361 cgaacaggca aggcaaaacc agtacttaca ggtcagctct tgcgtacccg ccctggaagg
      421 ctgcgatgtt aatggtgcca gttttaccct tgaacagatg ctcgcctggc gggaccatcc
      481 gcaggttacc ggccttgcag aaatgatgga ctaccctggc gtaattagcg ggcagaatgc
      541 gctgctcgat aaactggacg catttcgcca cctgacactg gacggtcact gcccgggttt
      601 gggtggtaaa gaacttaacg cctatattgc tgcgggtatt gaaaactgcc acgaaagtta
      661 tcagttggaa gaaggacgcc ggaaattgca actcggcatg tcattgatga tccgtgaagg
      721 gtccgcagcc cgcaatctca acgcgctggc accgttgatc aacgaattta acagcccgca
      781 atgcatgctc tgtaccgatg accgtaaccc gtgggagatc gcccatgaag gacacatcga
      841 tgccttaatt cgccgcctga tcgaacaaca caatgtaccg ctgcatgtgg catatcgcgt
      901 agccagctgg tcgacggcgc gccattttgg tctgaatcac ctcggcttac tggcacccgg
      961 caagcaggcc gatatcgtcc tgttgagcga tgcgcgtaag gtcacggtgc agcaggtact
     1021 ggtgaaaggc gagccgatcg atgcacaaac cttacaggcg gaagagtcgg cgagactggc
     1081 acaatccgcc ccgccatatg gcaataccat tgatcgccag ccagtttccg ccagtgactt
     1141 tgccctgcaa tttacccccg gaaaacgcta tcgcgttatt gaggtaatcc ataacgaact
     1201 gatcacgcac tcccgctcca gcgtctacag cgaaaacggt tttgatcgcg atgatgtgtg
     1261 ctttattgcc gttcttgagc gttacggaca acgactggct ccggcctgtg gtttgctcgg
     1321 cggctttggc ctgaatgaag gtgcgctggc ggcgacggtc agccatgaca gccataatat
     1381 tgtggtgatc ggtcgtagcg cagaagagat ggcgctggcg gtcaatcagg tgattcagga
     1441 tggcggcggg ctgtgcgtgg tccgtaacgg ccaggtacaa agtcatctac cgttgcccat
     1501 tgccgggcta atgagcaccg acacggcgca gtcactggcg gagcaaattg acgccttgaa
     1561 agccgccgcc cgtgaatgcg gtccgttacc ggatgagccg tttattcaga tggcgtttct
     1621 ttccctgcca gtgatccccg cgctgaaact aaccagtcag gggctgtttg atggtgagaa
     1681 gtttgccttc actacgctgg aagtcacgga ataataaaaa aaagcccggc gtcatgccgg
     1741 gcaaaagtca ccagttacgt ttatgccact gtcaactgct gaattttttt ctcgcggcgg
     1801 attttgcgtt cttccatcac cgccactatc gccatcaggc agatacaacc aatcgcggcg
     1861 atatccagcg cggcgaaggt gcctgcccag ccggtaaggc caaataccgg cgtcccatcg
     1921 gcaatcattc ccagacctaa cttggcaaag ctgtcaccaa tcaggtaagc gaaggtgcct
     1981 ttaataccat cggcagcgcc aatcgctttt ttaggtacaa agccaacagc cgccacgcca
     2041 atcaacagtt gcgggccaaa gaccaggaaa cccaacgcaa agagagaagc caggtagata
     2101 tattggttgc tggcgtgttg atacacaccg agcgtggcga taatcagcgc cagcgcgata
     2161 caggccacca ggccacggcg accgttcgcc aggtcagaga gccagcccca cagcagcgta
     2221 ccgaccagcg caccagcttc aaacagcgta aagccctgaa tcgccaccgc tttagagagt
     2281 ttcagttcct ggaacgcata cacagttgac cactggtcga taccaatacg caccacatag
     2341 aggaaaatgt tggcgaagca cagcagccag atcactttgt ttttcagcac atactcaaca
     2401 aagatctgcc acttggtcat atcggtagat tctgtctctt tatcctcttc actgatctcc
     2461 tcgccaaaca gctcttcagc tttgccgagg ccataagatt ccggggagtc gctgccgtaa
     2521 cgcagaccga taaaaccaac aatcagcgcg ataatcgacg ggaaaataaa catgccgatg
     2581 acatgaccat cgaacaggta atttgcgccg aacagcgcca cacctgctgc gcctgcacca
     2641 ccaaggttgt gagaaatgtt ccagaaaccg aggaatgtcc cgcgtttacg acgcggcgtc
     2701 catttggtga tggtggagta actgcatgaa ccgccggtac tctggaaaaa gccgctcaag
     2761 gcgtagaagg caatcatcag gaacaggcta accgagccgc tgcccatact ggcactgaag
     2821 cccagcatac aaatggcaga caggatcagc atgaacggca ggaattgttt ggtgtttttg
     2881 ccgtcggcgt agtaagaaac cagcgtttta cccacgccat aagtgatgga gaagcccagg
     2941 ccgatcatcc ccagctgcgt catgctcaac ccgtaggtcg agatcatgtc gttctgcgcg
     3001 atgttaaagt tcttgcgaat caggtacatc gtaaggtagc cgataaagac caccaggtag
     3061 gattgcatga acggtttgaa ccacattttg cgccgcactt cgagcggaag gtccagggtc
     3121 ggcttgcgaa cctggtttaa gaaagccagc atgggttact cctgaaatga atacctgcgt
     3181 gaggcatgca ttgtaaaaat cagcagcaaa tcaggcctga gatagcgtcc aggcaaaacc
     3241 tggaaaattt cttagttttg ccggatatga agtgaaaagg tgagatgcat cacgcttctc
     3301 gcggtgtctg ggcgttcaaa aagggcagta acagcagtgc ggaaatcccg gcggcgatgg
     3361 cgataaccac aaaaaatccg ctccagtgcc aggtatcgag tactttcgcc agcggccaac
     3421 cagcaagcga cgcaccaaga taagcaaaca agccgacaaa ccccgtcgcc gcccctgccg
     3481 cctctttgtg ggaacactct gccgccgcca tgccgattaa catctgcggg ccaaagacaa
     3541 aaaaaccaat ggtgaagaag caggttgcct gcatcacgta gctggcaaat ggcatcagcc
     3601 acagcgagcc gactgaaagc aaaattccgg cggcgaaaat caaattcatc ggcccgcggt
     3661 tgccgttaaa cagtttgtcc gaaccccaac ccgctaccag cgcaccgata aatccgccca
     3721 gttcaaacat tgtcactgcc gtattcgccg tgaccagatc gacgcccagc gtctcggaca
     3781 tatacaaatt gccccagtcg ttgatcgccg cccgaaccac atagaccagc acatagcaaa
     3841 acgaaagcag ccagatatac ggattcagca acacatattt ggtgaggatc tctttacgcg
     3901 tcagccctgc cccttcttgt tgttgagcaa tctccagtgc gtcgtgccgc cattcaccaa
     3961 ctgccggtaa acctaacgcc tgcgggcgat cgcgcagccg ccagcagaga aaaatcccca
     4021 cgacgatcgc catacaaccc gcaatcatca tcccggcacg ccagccgtaa tgcagcgcgc
     4081 tcgctgccat cacaatggga atgagtgcgc cgccgacgtt atgcgccgtg ttccataatg
     4141 cccaccaacc gccgcgctcg gtacgagaat accaggcggt taacagacgc gcacacaccg
     4201 gtgaacccca gccctggaaa aaggcgttca acacccagag cacggcaaac gcccatagcg
     4261 acgtcgagaa gccaaacaga atgttgataa tgcccgtggc aataagccct atccccataa
     4321 aataacgggc atttgagcga tcgctgacaa tgccggagac aaacttcgac acaccgtagg
     4381 taatgtaaaa cagggtcgct aacaggccaa tatcgctacg gctgagcacg ccgttagcaa
     4441 ggatttctgg tacggcggag ttaaaacttt tacgcgtgaa gtaaaacagc gcgtaaccca
     4501 gccagatggt cagcagaata tgccgacgcc agtagcgata gcgggcatca atttcatgtt
     4561 tatcagtcat taatggcgca tcggcaggcg ctttcagaaa cggcaacatc atcgaacctt
     4621 agacataacg ttgaggtaga gaaacgctga cacgcgtgcc gtgcaggcag gagatggtca
     4681 atgtgccacc cagcgccgtt acgcgctcgc gcattccggt aaggccaaaa ccgtgttgcc
     4741 cggaatctgg cggtaaaccg ctgccatcgt cttcaatcac cagcattaac cgctcatcat
     4801 gctgccagcc ttgcagcgtg accgcgctgg catcggcatg tttcacaatg ttgttcaacc
     4861 cttcctggca gacgcgaaat agcgtcacgc gctggttttc gcttaacgct gattcatcga
     4921 ttcgccattc gagatgactg acaatgccgc gcccttccag ctccatttcc cgcatcagtg
     4981 agcggatggc ctgctccagg gtgagatcat ccagctggcg cggacgtaac cgccctaaca
     5041 agcggcgcac cgcgtcgtaa acgcccaacg acaactgttc gatgagctgc ccgctctgtt
     5101 tcacgctggc gttatccgcc gcgagccgct ggacaatgcc agcctgagta cgaatagcag
     5161 tgatggtctg accgatatca tcgtgcagct ctcgcgccac atcgcggcgc acgctctctt
     5221 cggtttccag caaccgttca gcgagatgct gattgcgcgc cagttccttt tgcagcgact
     5281 ggttaagttc acgcaaccgc tggatgccag cgcccagtaa caaccctgtc agactttgca
     5341 ccagcagcga gagcaataaa tccaccggat gatcgcgcca ggtttgactg gcgatcagcg
     5401 cgatggcgtt catcaatgtc gcaatcagcg ctccttgcca accatagtgc caggccagcg
     5461 cgataatcgg cagcgccaga cagaatggcg taaagcgcga cagttcgtct ggcaatccca
     5521 actggagcca gagactgata acaaacagca gcaagtacca gaccagatgt cgcccgcgcc
     5581 agttgattgg ctgagaaacc agtgacgggc cgagcggcag ccaggtgtta ttggcgagat
     5641 agtgccagaa caccagacat atcggggcca gcgtcaggcc gccagttaaa gtcagcagca
     5701 gcgcattcca cgactctttg ccgtgccaaa gccagggcaa cgactgcaac aacgccgccg
     5761 ccgttaacgc cgcaccctgt aacagcaagg tgcgccagtc acgttgatgg cgatagcgcg
     5821 agatcagcgc cacgggcaat aacgtcagta aactaccgat catcaataag gagaaatggg
     5881 ttaaaccgac cgcctgcatt agccagtaaa tcagcagcca ctccgcaccc agtagcaccg
     5941 gccagtatcc gcgcgggcat tgcagcatta gccctagccg caggccaaac ggaaataaca
     6001 gcaccgccat atcagggcgc tcaaccaggt gcaggctgat actccacagg caaaaccatg
     6061 cggcagagaa gataaaaaag caggcaataa cggtaattaa acgggagaac aacgtcttca
     6121 tcaccagcca tcaaacatgc ggcgcgccag ttcaacgtcg ttactgacgc ccagtttttc
     6181 catcagattg gcgcgatgga cgtgtactgt tttcggcgac aagcccagtt cggcggcaat
     6241 ctctttcacc gccattcctt gcgccagttt ttctgccacc tggcgttcgc gtttggttag
     6301 cgggtcctgg cgaccagatg ccagtttaat ggcaatatcc ggcgtcagat aacagccgcc
     6361 agtggcaacc gtatgcaccg cagcaatgag ttcatccggg ctacagcgtt tggagagaaa
     6421 gccgcgcgcc cccgcgttaa gcgcctgctc aaccagcgcc ggactgtcgt gaacggagag
     6481 cataattgtc gccatgcctt tcggcagctg gcttagaagc tccagaccgg agatatcagg
     6541 catggagata tcgcaaatac acacctgcac accgcgcccc ggcagccccg ccagcgcctc
     6601 gcgccccgaa ccaaactcgg caactacctg caaatcaggt tccagcccca gcagctgcgc
     6661 aaagccggag cggacgataa ggtgatcgtc tataagggca acggtgatca tggtcttgtc
     6721 ctggcgggta ataaaacgcg cttaccttaa cgataagcgc gatgttgttc aagccttgag
     6781 cggttactgc caaaagctgc gctcttgttg cttgtggaaa gaagtgatgc cttcaaggac
     6841 ttgtatatcg actaaccatt aaaaa
//
LOCUS       DACADF010000208         6735 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.210, whole genome shotgun sequence.
ACCESSION   DACADF010000208 DACADF010000000
VERSION     DACADF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.210"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            8..1162
                     /gene="emrK"
                     /locus_tag="ISB30_004421"
     CDS             8..1162
                     /gene="emrK"
                     /locus_tag="ISB30_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015872332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAP1991984.1"
                     /translation="MNSNKKHSDRRKYFALLAVVLFIALSGAYAYWSMELKDMISTDD
                     AYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIV
                     RQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKEALEHTKDTLISS
                     KAALNAAIQAYKANKALVMNTPLNRQPQVIEAADATKEAWLALKRTDIKSPVTGYIAQ
                     RSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFH
                     GRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTAT
                     IDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            1162..2700
                     /gene="emrY"
                     /locus_tag="ISB30_004422"
     CDS             1162..2700
                     /gene="emrY"
                     /locus_tag="ISB30_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAP1991985.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIILLTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2808..4136)
                     /gene="dsdA"
                     /locus_tag="ISB30_004423"
     CDS             complement(2808..4136)
                     /gene="dsdA"
                     /locus_tag="ISB30_004423"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAP1991986.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKASQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4154..5491)
                     /gene="dsdX"
                     /locus_tag="ISB30_004424"
     CDS             complement(4154..5491)
                     /gene="dsdX"
                     /locus_tag="ISB30_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HAP1991987.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYTLLEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVIALAGTFLLSFII"
     gene            5709..6644
                     /gene="dsdC"
                     /locus_tag="ISB30_004425"
     CDS             5709..6644
                     /gene="dsdC"
                     /locus_tag="ISB30_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HAP1991988.1"
                     /translation="MEPLREIRNRLLNGWQLSKLHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLTHNFLMDEEILPVCSPEYAHRHDLTNTVINLRHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHVGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
BASE COUNT         1866 a         1537 c         1553 g         1779 t
ORIGIN      
        1 ggaacagatt aattcaaata aaaaacattc tgacagaaga aaatattttg ctttgttagc
       61 ggtagtttta tttattgcgc tttcaggtgc ctatgcctat tggtcaatgg aattaaaaga
      121 catgattagt acagatgacg cctatgtcac gggaaatgca gatccaattt ctgcacaagt
      181 ctcaggcagt gtcactgtcg ttaatcataa agatacgaac tacgttcgac aaggtgacat
      241 tttagtttca ctggataaaa ctgatgccac tatcgcactc aataaagcta aaaataatct
      301 ggcaaatatt gttcggcaaa cgaataaact atacttacag gataaacaat acagtgccga
      361 agtcgcttca gcacgtattc agtatcaaca atctttagaa gattataacc gtcgtgtgcc
      421 tttagcgaag caaggggtta tttcaaaaga agcgctggag cataccaaag atacgttaat
      481 aagtagcaaa gcggcattga atgctgctat ccaggcttat aaagcgaata aagctttagt
      541 aatgaacaca ccattaaacc gtcagccaca agtcattgaa gctgcagatg caaccaaaga
      601 agcctggttg gcgcttaaac gtacggatat taagagtccg gttaccggtt atattgccca
      661 gagaagtgtt caggtcggcg aaacagtgag tcccggacaa tcgttaatgg ctgtcgtacc
      721 ggcgcgtcaa atgtgggtta atgccaactt taaagaaaca caactcacgg atgtacggat
      781 tggtcaatcg gtcaatatta tcagcgatct ttatggtgaa aatgttgtgt ttcatggtcg
      841 ggtgacaggg atcaatatgg gaaccggcaa tgcgttctcc ttattacctg cacaaaatgc
      901 gacagggaac tggatcaaaa tcgttcagcg tgtaccggtt gaagtttctc tggatccaaa
      961 agaactcatg gaacaccctt tgcgcattgg tttatcgatg actgcaacta ttgatacgaa
     1021 gaatgaagac attgccgaga tgcccgatct ggcttcaacc gtgacgtcca tgcctgctta
     1081 taccagtaag gctttagtta tcgataccag tccgatagaa aaagaaatta gcaacattat
     1141 ttcgcataat ggacaacttt aatggcaatc actaaatcaa ctcctgcacc attaaccggt
     1201 gggacgttat ggtgcgtcac tattgcattg tcattagcga cgtttatgca aatgttggat
     1261 tccactattt ctaacgtcgc aataccgaca atatctggct ttctgggagc atcaacagac
     1321 gaaggtacct gggttatcac ctcgtttggc gtagcaaatg ccattgcgat ccctgttacc
     1381 ggcaggctgg cacaaagaat aggcgaatta aggttatttt tactttcagt aacctttttt
     1441 tcgctgtctt cattaatgtg tagtttatcg accaatcttg atgtgctgat attttttaga
     1501 gtcgttcagg ggttaatggc ggggccgtta attccactgt cacagagttt attattaagg
     1561 aattatccac cagaaaaaag aacatttgct ctggcattat ggtcaatgac cgtgattatc
     1621 gctccgatat gtgggccgat attgggcggc tatatttgcg ataactttag ctggggttgg
     1681 atatttttaa tcaatgtccc tatggggatt atcgtcctga cattatgctt aaccttactt
     1741 aaaggacgag aaactgagac ttcaccggtc aaaatgaatc taccaggact gaccctgtta
     1801 gtgctcggtg ttggtggctt gcaaattatg cttgataaag ggcgcgatct ggattggttc
     1861 aactcgagta caataatact actaacagta gtatcagtta tttctctgat ctctttagtc
     1921 atttgggagt cgacctcaga gaatccgatt cttgacctca gtttgtttaa gtcccgtaat
     1981 ttcaccattg gtattgtgag tatcacatgc gcgtatttat tttactctgg agcgatagtt
     2041 cttatgccgc agttactcca ggaaacgatg gggtataatg cgatatgggc cggacttgcc
     2101 tatgcgccca tcggtatcat gccgctatta atatcacctt tgataggacg ttatggcaac
     2161 aaaattgata tgcggttgtt ggtgacattt agttttttga tgtatgcggt ttgctattac
     2221 tggcgttctg tgacatttat gccaacgatt gattttacag gcatcatttt gccacagttt
     2281 tttcagggat tcgccgttgc ctgtttcttt ttacccttaa caacgatttc gttttcaggc
     2341 ttgccagata ataaatttgc caatgcctcg agtatgagta atttttttcg caccttgtca
     2401 ggatcagttg gtacgtcgtt gacaatgaca ctgtggggac gccgcgaatc gttacaccat
     2461 agtcagttga cagcaaccat cgatcaattt aaccccgtgt ttaattcatc gtcacaaatt
     2521 atggataaat actatggttc gctttcagga gttcttaatg aaattaataa tgaaataact
     2581 cagcagtcac tttctatttc tgcaaatgag attttccgta tggcggctat tgcttttatc
     2641 ttacttacgg ttttggtttg gtttgcgaaa ccgccgttta cagcgaaagg cgttgggtga
     2701 tggaaaaaga ggaggctata gcaggtaaaa cggcagaatc ttaagtctgc gtcatcggca
     2761 gcatcgctca cccagggaaa ggatggtgat gctgcgttga aacgttatta acggcctttt
     2821 gccagatatt gattcatctc ttcttccggc accattccac ctcccgtcgc ccacaccaga
     2881 tgagtggcat tacgcagttg ttctgcgctg aaaccgtgca tctgttggta acttactgat
     2941 gcgcacacgc gctgaggtcc ggccataccc gccagtgccg aaggttcaag acgaatacct
     3001 tcttcctgcg ccagccagcc aagcatgtca tacatggttt gatcgctaag ggtatagaag
     3061 ccatccagca gacgctccat cgcccgcccg acaaagcccg atgcgcgacc aaccgcaagg
     3121 ccatcggcag cggtaaggtt gtcgatgcca atatcctgaa cagaaatctg atcgtgtaat
     3181 ccggtatgga cgcctaataa catacacggg gagtgtgttg gttcggcaaa aaagcagtga
     3241 acatgatcgc caaacgccag tttaagtccg aatgcgacgc caccaggacc accgccaaca
     3301 ccacacggca gatagacaaa tagagggtta tcagcatcga cgatacggcc ttgctgggca
     3361 aattgcgctt taagacgctg gccagcgacg gaatacccaa ggaacaacgt gcgggaattc
     3421 tcgtcatcaa taaagaaaca gttcgggtca gactgcgatg ctttacgtcc ttcctcgaca
     3481 gcaacaccat aatcttgctc atattccacg accgtaacgc catggctgcg cagtttcgct
     3541 tttttccatg cccgggcatc agcagacata tgcactgtca ccttaaagcc aatgcgggcg
     3601 ctcataatac cgattgataa ccccagattt ccggttgagc ccacagcaat gctgtattgg
     3661 ctaaagaact gtttaaactc cggagaaagc agtttgctgt agtcatcttc aagcgtcagc
     3721 aaccctgctt ccagagccag tttttctgca tgtgccagga cttcataaat cccgccgcgt
     3781 gcttttatgg agccggaaat aggcaaatgg ctatcttttt tcagtaacag ttgcccgctg
     3841 atcggttgct gatattcctt ttccagccgt ttttgcatag ccggaatggc aaccagttct
     3901 gattcaataa tccccccagt ggcagcagtt tcaggaaatg cttttgccag atagggggca
     3961 aaacgagata agcgcgcatg ggcgtcctga acatcctgtt cggtcaggcc aacataaggt
     4021 aaaccttcag ccaatgaggt cgtgccagga ttgaaccaag tggtttcttt aagagcaacc
     4081 agatccttta ccaacggata ctgggcgatg agcgaattca ttttagcgtt ttccatagta
     4141 cgtctctttg cgcttagatg ataaatgaca gcaggaatgt gcccgccaga gcgatgaccg
     4201 aagcgataaa tgtcgctgtc gtatagtatt taaatgtttc attgagcgta gcgccgcaat
     4261 attgcttcac cagccagaaa agcgagtccg taacgatcgt gcagccaatc gcaccggaac
     4321 cgatagcaat cgcaataatt tccgggctga tgtcgggata cagcggcagc atgggggcaa
     4381 caattgccgt tgcccccatc attgccaccg tagcggagcc cactgcagca tgcagaataa
     4441 gtgccaccaa ccaggccaga agaatcgggt gcatatgcat attggagaga ataactgcca
     4501 gcgtatcagc gagactgctg ctttttaaaa tggcgttgaa tgcgcctccg gccccgataa
     4561 tcagcaaaat attagcaata gaaccgaaac cattttccgt atgtgtgagc atcgtcccca
     4621 tgctcatatg ctgacgtata cccaacacat aataggcgac aaacacggcg ataaacatgg
     4681 cagtgatagg gttgccaata aactcaagca aggtatacaa accactctca cgcgccatat
     4741 tcaattcggc aatcgtttta accaacatca gcgcaatggg tagcagtatg gtgaataacg
     4801 ttgcgcctaa tgaaggtagt gttttttcat cgcgaacttt gagatcggca aactctgtgg
     4861 gtacaggttt aaagggcagt cgttgcccca gaaatttaag gaaaagtggg ccaccgatca
     4921 gtgatgccat cagcccaacc agcaaaccgt agacgatcac cgaaccgata tctgcgccca
     4981 gcttattggc aacatataaa gcagccggat gtgggggaac cacgcagtgc acagccatca
     5041 atgcggtaca cagcggaatg gccagcttta acaatgaggt attggttttt ttggcaattg
     5101 aaaaagccag aggaatcaat agcacgacgc ccacttcaac aaacagcgtg atgccgcaaa
     5161 tcaggccaac cagcaccata atgacatcag ccgaaagcca ccggcagcgt tgaagtgtca
     5221 gaccaattct ttctgcggcc ccggatactt ccatcatttt tcccagtatc gtgccaaggc
     5281 cgataaccgc agcgaggaac cccagcgttc cgccaattcc actttcaata gcatttacca
     5341 tatccagtgg ccccataccc atcatcgttc ccacgaagaa gctggccagc aacagcgcca
     5401 gaaacgggtg gaatttgaac ttcacgatgg tcagtacaat taacacgatg ctgataagca
     5461 gcgtgctcac aacccagatt tgagagtgca tatctcacct tcccctgtga ttaacctgat
     5521 gatattggac aaaaaaacaa cgcaggctga caaacgataa aatttgcctt tcacatgagc
     5581 taaattgcat caagggagtg atttacatct aaaaatgaag gtgaattgag atatggttca
     5641 ctttagctca ccttacagct gaaattttgc cgctattttt ttacacttaa tagaaaaatg
     5701 aggtgattat ggaacccctt cgtgaaataa gaaatcgact gcttaacggc tggcaactat
     5761 caaaactgca tacttttgaa gtggctgcca ggcatcagtc tttcgccctg gcggcagagg
     5821 aattgtcgct gagccccagt gcggtaagtc accgtatcaa tcagctggaa gaagaattag
     5881 gtattcagtt gtttgttcgt tcccatcgca aagtggaatt aacgcacgag gggaaacgcg
     5941 tttattgggc gctaaaatcg tcgctggata ccctgaatca ggaaattctg gatatcaaaa
     6001 atcaggagtt atcgggaacg ttaactctgt attcccggcc ctctatcgcc caatgctggt
     6061 tggtgcccgc attaggtgac tttacacgcc gatatccgtc tatttcgctc accgtgctca
     6121 ctggtaatga caatgtcaat ttgcaacgtg ccggaatcga tttggcgatc tactttgatg
     6181 atgcgccgtc agcgcaactg acccataact ttctgatgga tgaagaaatc ttgccagttt
     6241 gtagcccgga gtacgctcat agacatgatt taaccaacac ggtaattaac ctgcgtcact
     6301 gtacgttgct ccatgacaga caggcatgga gcaacgactc cggtacggat gaatggcata
     6361 gttgggcgca acattatgcg gttaatttgc cgacatcttc tggaattggc tttgatcgtt
     6421 ctgatttagc cgttatcgcc gcgatgaatc atgttggggt ggcgatggga aggaaacgcc
     6481 tggtacaaaa aaggcttgcc agtggtgagc tcgtcgcgcc gtttggcgat atgacggtga
     6541 aatgccatca gcattattac atcaccacat taccgggcag gcagtggcca aaaattgagg
     6601 catttattac ttggttaaga gaacaggtaa aaaccacttc ataatgaata agaccacgag
     6661 ttcctggtgt gccttattcg gcactgctag tgcgaattta ataatttacg gtttttacgt
     6721 ggttttaggg ataaa
//
LOCUS       DACADF010000209         6708 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.211, whole genome shotgun sequence.
ACCESSION   DACADF010000209 DACADF010000000
VERSION     DACADF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.211"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..907
                     /locus_tag="ISB30_004426"
     CDS             <1..907
                     /locus_tag="ISB30_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAP1991989.1"
                     /translation="LFFVLGGPIVAHIFSSHYESYDLHIAELTNENGQVVWKASYVTI
                     MIFMWLTLLSVNLYFNGLRCDIWNGVSVIAFCAVLYNISLLFMSRYSVSIWYISRTIE
                     VVSKLTVLVIFMCHIFSALRVTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCV
                     MIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTEIDT
                     ECAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQKNELNPKEIYQLADNALY
                     EAKETGRNKVVVKEAEILINRKDDE"
     gene            complement(1012..1099)
                     /locus_tag="ISB30_004427"
     tRNA            complement(1012..1099)
                     /locus_tag="ISB30_004427"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1063..1065),aa:Ser,seq:gga)
     gene            1333..2271
                     /gene="ghrA"
                     /locus_tag="ISB30_004428"
     CDS             1333..2271
                     /gene="ghrA"
                     /locus_tag="ISB30_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HAP1991990.1"
                     /translation="MDIIFYHPTFDTQWWIEALHKAIPQARVRAWKSGDNESADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRLQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            2326..3063
                     /locus_tag="ISB30_004429"
     CDS             2326..3063
                     /locus_tag="ISB30_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAP1991991.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKYQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3087..3641
                     /locus_tag="ISB30_004430"
     CDS             3087..3641
                     /locus_tag="ISB30_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP1991992.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3743..4234
                     /locus_tag="ISB30_004431"
     CDS             3743..4234
                     /locus_tag="ISB30_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAP1991993.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSTKTAHREQQIK
                     NKA"
     gene            complement(4299..5132)
                     /gene="csgG"
                     /locus_tag="ISB30_004432"
     CDS             complement(4299..5132)
                     /gene="csgG"
                     /locus_tag="ISB30_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HAP1991994.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKTERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5159..5575)
                     /gene="csgF"
                     /locus_tag="ISB30_004433"
     CDS             complement(5159..5575)
                     /gene="csgF"
                     /locus_tag="ISB30_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HAP1991995.1"
                     /translation="MRVKHAVVLLMLISPLSWAGSMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5600..5989)
                     /gene="csgE"
                     /locus_tag="ISB30_004434"
     CDS             complement(5600..5989)
                     /gene="csgE"
                     /locus_tag="ISB30_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAP1991996.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(5994..6644)
                     /gene="csgD"
                     /locus_tag="ISB30_004435"
     CDS             complement(5994..6644)
                     /gene="csgD"
                     /locus_tag="ISB30_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAP1991997.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1720 a         1507 c         1638 g         1843 t
ORIGIN      
        1 tttgtttttt gttcttgggg ggccgatagt tgctcacata ttttctagcc attatgagag
       61 ctacgattta cacattgccg agttaaccaa tgaaaatggt caggtggttt ggaaagcctc
      121 atatgttacc ataatgattt tcatgtggtt aaccttgctt tcggttaatc tatactttaa
      181 tggtttacgc tgtgatatct ggaacggggt gtcggtgatt gctttttgtg ccgtgctgta
      241 caatatctct ctgttattta tgagcaggta tagcgtgtcc atttggtata ttagtcgcac
      301 tattgaagta gtcagtaagt taaccgtttt ggtaattttt atgtgccata tattcagtgc
      361 gctacgagta acaaaggaca ttgcacatcg cgatccctta accaatatat tcaacagaaa
      421 ttattttttt aatgaactga cggttcaatc agcatcggcc aaaaaaacac cttattgcgt
      481 catgattatg gatattgacc acttcaaaaa agtcaacgac acctggggac atccggttgg
      541 cgatcaagtg ataaagacag tggtgaatat cattgggaaa agcatacgac cagatgacct
      601 tttagcgcgc gttggcggcg aagagtttgg cgtcttgctg acggagatcg atactgaatg
      661 tgcgaaagct ttagcggaaa ggattcggga aaatgttgaa cgtttaactg gcgataatcc
      721 tgaatacgcc ataccacaaa aagtgacgat tagtattggc gctgttgtaa cgcagaaaaa
      781 tgagttaaac cctaaggaga tctatcaact ggctgataac gcactttacg aggcgaaaga
      841 gaccgggcgt aataaggtgg tagtaaaaga ggcagaaatt cttatcaacc gcaaggatga
      901 tgaatgagtc tgtcgctagt tgtcaacctc cgcctggggc ttctcatccc cccggtgtat
      961 gcaatatacg aaaaaaaagc tcgtactttc gtacgagctc ttctttaaat atggcggtga
     1021 gggggggatt cgaacccccg atacgttgcc gtatacacac tttccaggcg tgctccttca
     1081 gccactcgga cacctcacca aattgtcgtt cctgtcttgc tggaacgggc gctaatttag
     1141 ggaaatcatg acctgaggtc aacaaacttt tttaaaaaat cgctcgatta ttcaaacttc
     1201 aatcaatatg tagttttaat aagcgaaatt tgcttttttt gtcaccgacc acggatttgt
     1261 tatgctggtg gcctttgtag atcataacga taagtgcgaa taaatttcgc gcaacgcttt
     1321 tcgggagtca gtatggatat catcttttat cacccaacgt tcgataccca atggtggatt
     1381 gaggcactgc acaaagctat tcctcaggca agagtcagag catggaaaag cggagacaat
     1441 gaatcagccg attatgcttt agtctggcat cctcctgttg aaatgctggc agggcgcgat
     1501 cttaaagcgg tgtttgcact tggggcaggt gttgattcta ttttgagcaa gctacaggct
     1561 caccctgaaa tgctgaagcc ttctgttcca ctttttcgcc tggaagatac cggtatgggc
     1621 gagcaaatgc aggaatatgc tgtcagtcag gtgctgcatt ggtttcgacg ttttgacgat
     1681 taccgcctcc agcaaaatag ttcgcattgg caaccgctgc ctgaatatca tcgggaagat
     1741 tttaccatcg gcattttggg cgcaggcgta ctgggcagta aagttgcaca gagtctgcaa
     1801 acctggcgct tcccgctgcg ttgctggagt cgaacccgta aatcgtggcc tggcgtgcaa
     1861 agctttgccg gacgggaaga actttctgca tttctgagcc aatgccgggt attgattaat
     1921 ttgttaccga atacccctga aaccgtcggc attattaatc aacaattact cgaaaaatta
     1981 ccggatggcg cgtatctcct caacctggcg cgtggtgttc atgtcgtgga agatgacctg
     2041 ctcgcggcgc tggatagcgg caaagttaaa ggcgcaatgc tggatgtttt taatcgtgaa
     2101 cccttaccgc cggaaagtcc gctctggcaa catccacgcg tgacgataac accacatgtc
     2161 gccgcgatta cccgtcccgc tgaagctgtg gattacattt cgcgcaccat tgcccagctc
     2221 gaaaaagggg agagggtctg cgggcaagtc gaccgcgcac gcggctacta ataaagcatc
     2281 aggtttcctg ctatccttgg cgggaattga atacaggaga gagttatgta tcccgtcgac
     2341 cttcatatgc ataccgttgc cagcacgcat gcatatagca cattgagtga ttacattgcc
     2401 caggccaaac aaaagggcat taaacttttt gcgatcaccg atcatggccc ggatatggaa
     2461 gatgcgccgc atcactggca cttcattaac atgcgtatct ggccgcgagt ggttgatggg
     2521 gtagggatcc tgcgcggcat cgaagctaac attaaaaatg ttgatggtga aattgattgc
     2581 agcggcaaaa tgtttgattc gctggatctg attattgccg gttttcatga gccggttttt
     2641 gcgccacatg acaaagcaac caatacacaa gcgatgatcg ccactatcgc cagcggcaat
     2701 gtgcatataa taagccatcc cggaaatccg aaatatgaga tagatgttaa agccgttgct
     2761 gaagcagccg cgaaatatca ggtggcgctg gaaatcaata attcctcatt tttacactca
     2821 cgtaagggca gtgaagacaa ctgtcgtgcg gtagctgcag cggtacgtga cgctggaggt
     2881 tgggtggcat taggctcgga ttctcacact gcatttacca tgggggaatt tgaagagtgt
     2941 cttaaaatcc tcgacgcggt agattttccg ccagaacgca ttttgaatgt ttctccgcgc
     3001 cgcttactga acttccttga atctcgcggt atggcaccga ttgcggaatt tgcagacctt
     3061 taattactag taatggaaat atattaatga acgagttttc tatcctctgt cgtgtgctgg
     3121 gttcgctcta ttaccgccaa ccgcaagatc ctttactggt gccgttgttt accctgattc
     3181 gtgaggggaa actggctgcg aactggccac tggagcagga tgagttactg acgcgtttac
     3241 agaaaagctg tgatatgacg caagtctctg ccgattacaa tgcgttgttt atcggcgatg
     3301 aatgtgctgt gccgccatat cgtagcgcat gggttgaggg cgcaacggaa gcggaagtgc
     3361 gcgcttttct ttccgaacga gggatgccac tagcggatac gccagccgat cacatcggca
     3421 cattgctgct tgcggcttcc tggctggaag atcagtcaac ggaagatgag agcgaagcac
     3481 tggaaacact gttcagtgag tatctgttac cctggtgtgg tgcgttcctt ggcaaagtgg
     3541 aggcccatgc aactacgcct ttctggcgca ccatggcacc gctaacccgc gatgctatta
     3601 gtgcaatgtg ggacgagctg gaagaagatt ctgaagagta aatgtgatct gcatcactca
     3661 taactgcaac tgataatttg ttgttgcata aaatgtgtgc tcgatctcat tcatggccgc
     3721 gttttctgct atcatgcgcg gcatgaacat acttctctct attgcaatca caacaggcat
     3781 tctctccggt atctggggat gggtggctgt ttccctgggc ttactaagct gggcgggctt
     3841 cctgggctgt acggcctact ttgcctgccc gcaaggtggg ctgaaagggc tggcgatctc
     3901 cgctgcaacc ctgcttagtg gcgtggtgtg ggcgatggtc attatttacg gtagtgcact
     3961 ggcaccacat ctggaaattc tcggttatgt aataaccggt attgtcgcgt ttctgatgtg
     4021 tattcaggcc aaacagctgc tgctttcatt tgttccgggg acgtttatag gcgcatgcgc
     4081 gacgtttgcc gggcaggggg actggaaact ggtgttacct tcgctggcgc ttggactggt
     4141 atttggttac gcaatgaaaa acagcggcct gtggctggcg gcgcgtagta cgaagaccgc
     4201 tcaccgtgag cagcaaatca aaaataaagc gtgaggggca ctcacgcttt cgcttaaaca
     4261 gtaaaatgcc ggattattaa ttccggcgtt tttatctatc aggattccgg tggaaccgac
     4321 atatggcggt atttcaccag aatgtcattc tgccgttctg ttttattttg caaatcccaa
     4381 aggccgcggt cgataccatc attaatcagg aaaatgacgc ctgtttcgat agccgacatc
     4441 aggcacagca taacgggttc gttcgaggtg taacctactt ccccttccag caagcgctgg
     4501 tagtcaataa agcggaagac ccccgcctga acttcatagg aaagtatcgt cttactggtg
     4561 ttcaccgagg agaggatctc accggtactc acattgacga cacgcaggtt cacggcaatc
     4621 tgatcaagct ggtattgcgt gtctgcaccg atcccaaaat atcttgcccc aacaccgccc
     4681 gatttaacgt tgctttcata accgataatc gaaccttcaa ccatgatatt tgccgccgtt
     4741 aaagattgca gcgggattcg gttattaatg gcaactgtgc cgttttcttg tgccgcacga
     4801 ataatcttgc gctcgttaag cagattttgt aagccctggc gctccagcgg tataaaccag
     4861 cgagaatctt tcagtgccgt gaccagcatt gccgtggcgc tttgcggaac agcagtggag
     4921 aagttacttg ccgggtaggg tttaaattgt ccggtttcgt cctgaatgtt gtataccgaa
     4981 acaaagatct tacccgtcgg cgctggcaga tgggtcaaat ctttgtagct ctgagcacga
     5041 ggcattaatg tcggtctggc ggcttcttta ggcggggcgg ttaagcatcc gctcagtaac
     5101 atgacggcaa ccaaaagaaa taagcgctgc atgattattt tccttatgaa gctggggctt
     5161 aaaaatcggt tgagttattt tgtaaacccg aaacctggat ggtcgaggtt tgtccggttt
     5221 tacgatctgt cacgttcaac tgcaattgac catcgcggtt agcaatatca acaatataat
     5281 cgttggtcac catgcggccc ggtttaccgg tattaatatt cgacagtagc ccacccaaaa
     5341 tttgtgactg gatggcctga gtaaagttat ctaacgctga tggcgtttca ataccaaagt
     5401 catcgttata gctcggatct ttataagagt tttgtgcctg agcgctattt aacaaaaaag
     5461 cgccattatt tgggttacca ccaaagtttg gattacggaa ctggaaagtc atggatccag
     5521 cccaacttaa tggcgaaata agcatgagta gaactactgc atgtttgaca cgcattgcag
     5581 cctccgaaca atttattatt taaaattcat catgcgccaa atcgcccgta cttaataacg
     5641 cctggtttat ctggcgacga tttagtgctt cttcagtttg aatcagtgca aagacgacag
     5701 ttttctcgaa gtctcttttc aacggaaata aaaaagtctg gaaaataacg tcctgattga
     5761 ccgttatagt gatccaactt ccccatcgtg cactgggcct ttcattaatc gttaagttac
     5821 ccgtatagtc actttcccat ttatcactaa aggctcggta aaaatcatgg ccaatagatg
     5881 aaacagtatg gtcagttaac aatcccggga cttctacctc aacggcatga agattccctg
     5941 cggcgaacag aaattctgcc gccacaatcc agcgtaaata acgtttcatg gctttatcgc
     6001 ctgaggttat cgtttgccca ggaaaccgct tgtgtccggt tttttacggc tatcttcttg
     6061 aaaagattat aaagatgcgt tttaaccgta ttttcgctga tgaacaacga acgagcgatc
     6121 tcgttattag acgcgccgat acgcagctta ttcaggatct ctttttcccg atgagtaagg
     6181 agggctgatt ccgtgctgtt ataacgatag ttacctgaat gcgtaatcag gtaactggca
     6241 agcttttgcg taaagtagca ttcgccgcgc aggacgcctt gcaacccatt gacaacacgt
     6301 tcttgatcct ccatggcata aaaaacgccg ttgatatgag gccagttttc aatgtcgcgg
     6361 tacgggtaat cttcaggcgt atttagcaac aatattttga tattgttgtt tttcctgctc
     6421 aaagtatcct gccaataatg gataagcttt ttatccgctt ccatcatatc cagaagaata
     6481 atagagcctg aagatatatc gtccagagaa cgttgaatat tatgtaattt tcctgtaatc
     6541 gccagcgatt gtttaaggtg ctgcaagaga gctgtcgctt gcaaagaagg tttagtgatc
     6601 aacaataatg tatgaccatg aatactatgg acttcattaa acatgatgaa accccgcttt
     6661 tttattgttc gcacacctga cagctgcctc taaaatagaa gcaccaga
//
LOCUS       DACADF010000210         6669 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.212, whole genome shotgun sequence.
ACCESSION   DACADF010000210 DACADF010000000
VERSION     DACADF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.212"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            264..803
                     /locus_tag="ISB30_004436"
     CDS             264..803
                     /locus_tag="ISB30_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP1991998.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQPQPEPQSEAGTKKGGAKTKGRGRKAAQPE
                     PQPQPPEGEDYGFSDDIPF"
     gene            866..1099
                     /locus_tag="ISB30_004437"
     CDS             866..1099
                     /locus_tag="ISB30_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP1991999.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1165..3123
                     /locus_tag="ISB30_004438"
     CDS             1165..3123
                     /locus_tag="ISB30_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP1992000.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVILQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWGWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVGGISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGVAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NCWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3178..3612
                     /gene="psiB"
                     /locus_tag="ISB30_004439"
     CDS             3178..3612
                     /gene="psiB"
                     /locus_tag="ISB30_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HAP1992001.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3609..4328
                     /locus_tag="ISB30_004440"
     CDS             3609..4328
                     /locus_tag="ISB30_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HAP1992002.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARV
                     VGHAVREMERWMVPNKLREAA"
     gene            4574..5943
                     /locus_tag="ISB30_004441"
     CDS             join(4574..5054,5054..5943)
                     /locus_tag="ISB30_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HAP1992003.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
     gene            6054..6212
                     /locus_tag="ISB30_004442"
     CDS             6054..6212
                     /locus_tag="ISB30_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAP1992004.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
BASE COUNT         1594 a         1674 c         1975 g         1426 t
ORIGIN      
        1 ccacaaccgt ctccgccagt ccaggggtga aatgcacatc ccgcaaaact ttttgcccag
       61 aagcaggcaa aaacttttgc ggtctgcccc cggacagtcg tttccgtccg tggcagtgat
      121 gccccatgcg gcgaaggttc tcaaaaaggt gatggtgaat accggaggga acaaaaggca
      181 ggtccccgca aaacgtcaaa accgcggctc ccgaaactca aaagatgatg ggggctgaaa
      241 acatcaacag aaggaggcac atcatggcag ttcgtggcat taacaaggtc attctcgtag
      301 gacgcctggg aaaagatccg gaagtccgtt acatccccaa cgggggcgca gtggcaaacc
      361 tgcaggtcgc cacgtcggaa acctggcgtg acaaacagac aggggagatg cgggagcaga
      421 cagaatggca tcgcgtggtg ctgttcggca agctcgcgga agtggctggc gaatatctgc
      481 gcaagggcgc gcaggtctac atcgaaggtc agcttcgcac ccgtagctgg gaagataacg
      541 gtatcacccg ttacgtcacc gaaattcttg ttaagaccac aggcaccatg cagatgctgg
      601 gacgtgccgc aggtgctcag actcagccgg aagaggggca acagttcagc ggtcagcctc
      661 agccggaacc acagtcggag gccggtacga aaaaaggtgg cgcaaaaacg aaaggccgtg
      721 gacgtaaggc cgcgcagccc gagcctcagc cgcaaccgcc ggagggggag gattacgggt
      781 tttcagacga tatcccgttc tgatcgggct gactgtgata accgccccgc cccgttctgt
      841 gcggggcatc accggagaga tgagaatgag cgaatatttc agaatacttc aggggctgcc
      901 tgacggtcct tttacccgca aacatgccga agctgttgcc gcacagtacc ggaacgtctt
      961 tatcgaggat gaccacggcg aacagtttcg cctggttgtc cgtaataacg gtgcaatggt
     1021 ctggcgtacc tggaattttg aggacggtgc cgggtactgg atgaaccatg tcatcaggga
     1081 tttcgggatt cttaagtaag agaggtgccg gacggggaca cgtccggcag acaatcatta
     1141 ttaccgtttt gggaggtact aactatgtca gttacagagt ctaaggcaaa aacggagcgt
     1201 aaatccagcc gtaaacctgc aaaaacgcag gaaacagtcc tgtctgccct gctggcgcag
     1261 acggaggaag tgagcgtgcc gctggcctca ctgattaaat caccgctgaa tgtgcgcacg
     1321 gtgccgtatt ctgtggagtc cgtcagcgaa ctggctgagt ccattaaggg cgttggcctg
     1381 ttgcagaatc tggtcgtgca taccctgcct ggtgaccgtt acggtgtcgc cgcaggtggt
     1441 cgccgactgg ctgcactcaa catgctggca gagcgcaaca tccttccggc tgactggcct
     1501 gtacgcgtga aggttattct gcaggaactg gcgactgccg catcaatgac tgaaaacggt
     1561 catcgtcggg atatgcaccc tgccgaacag attgccggat tccgcgcaat ggcgcaggaa
     1621 ggcaaaacac ctgcgcagac tggcgacctg ctgggctatt caccccgcca cgtccagcga
     1681 atgctgaaac tggcagacct tgcacccgtc atccttgatg cgctggcaga agaccgcatc
     1741 accaccgaac actgtcaggc gctggcgctg gagaacgaca ccgcgcgtca ggtgcaggtg
     1801 tttgaagccg cctgccagtc aggatggggc ggtaaaccgg atgtgcgggt tatccgcaac
     1861 ctgattaccg aaagtgaagt ggcggtggct gggaacagta aattccgctt cgtgggggct
     1921 gatgccttct cgccagacga actgcgcacc gatttgttca gcgacgacga gggtggctat
     1981 gtggactgcg tggcgctcga tgccgccctg ctggaaaaac tccaggctgt cgcagagttc
     2041 cttcgggaag ccgaaggctg gggatggtgt gccggacgca tggagcctgt aggtgagtgc
     2101 cgtgaggatg ccggaacata ccgctgtctg ccggagccgg aagcggtgct gacggaggcg
     2161 gaagaagaac gtctgaacga actgatgacg cgttacgacg cgctggaaaa ccagtgtgag
     2221 gaatctgacc tgctggaagc agaaatgaag ctgatgcgct gcatggcgaa ggtcagagcg
     2281 tggacgccgg agatgcgtgc cggaggtgga gtggtggtgt cctggcgtta tggcaacgtg
     2341 tgtgtccagc gaggtgtgca gttgcgcagt gaggatgacg tggctgacga cgctgaccgc
     2401 acggaacagg tgcaggagaa agcgtcagtg gggggaatca gtctgccact gctgacgaaa
     2461 atgtcttccg aacgcacgct ggcagtccag gcggcgctta tgcagcaacc ggacaaatct
     2521 ctggcactgc tggcatggac gctctgcctg aatgtgtttg gcagcggagc gtacagtaaa
     2581 ccggcacaaa tcagcctgga atgtaaacat tattcgctga ccagcgatgc gccgtcaggg
     2641 aaggaaggtg tcgcattcat ggcgctgatg gcagaaaaag cccgtcttgc tgccctgtta
     2701 ccggagggat ggtcacgaga catgacgaca ttcctgtcac tcagtcagga ggtgctgttg
     2761 tccctgctca gtttctgcac cgcatgcagc cttaacggtg tccagacccg tgagtgtggt
     2821 cacacgtcac gcagtccgct tgactcgctg gaaaccgcta tcggattcca catgcgcgac
     2881 tggtggcagc cgacaaaagc aaacttcttc ggacacctga aaaagccgca gattatcgca
     2941 gccctgaatg aggcaggact gtccggtgct gcacgggatg cagagaagat gaagaaaggt
     3001 gatgcggctg aacatgcaga gcaccatatg aaagacaact gctgggtgcc tggctggatg
     3061 tgtgcaccac atccacagac ggataccact gaacgcaccg ataacctggc tgatgccgcc
     3121 tgataaacaa ccacaccgcc ccgccggaga cggggcggca gcaagggaga taccgtgatg
     3181 aaaactgaac tgaccctgaa tgtattacag accatgagcg cacaggaata tgaagatatc
     3241 cgggcagccg gaagtgatga acgtcgtgag ctgacacacg ccgtgatgcg ggaactggat
     3301 gcgccggaca actggatgat gaatggcgag tatggcagtg agttcggggg ctttttcccc
     3361 gtccaggttc gtttcacgcc agcccacgaa cgtttccacc tggcgttatg ttcaccgggc
     3421 gatgtctctc aggtctgggt gctggttctg gtgaatgccg gtggtgagcc gtttgcggtg
     3481 gttcaggtac agcggcggtt tgcaccggaa gccgtcagcc attcactggc actggcggcg
     3541 tcactggacg cgcaggggta cagcgtcagt gacatcatcc atatcctgat ggcggaagga
     3601 ggtcaggcat gagcgcacgt tcacaggcac tggttcctct cagcacagag caacaggccg
     3661 catggcgggc ggtggcggag accgaaaaac gccgccatca gggtaacacg ctggctgaat
     3721 atccgtatgc gggggcgttt ttccgctgtc tgaacggaag ccgcaggata tcgctgtctg
     3781 acctgcgatt tttcatgccg tccctgaccg cagaagaact gcacggcaac cgtcttcagt
     3841 ggctgtatgc catcgatgtg ctgattgaaa cacagggcga ggtctgcctc cttccccttc
     3901 ccggtgatgc agcagagcgc ttgttcccgt cggtgcggtt tcgtgtcagg gaacgcagtc
     3961 gccataaatc cgcgctggtc atgcagaagt acagccgcca gcaggcgagg gaggcagagc
     4021 agaaagcccg tgcgtaccag gcgctggtcg ctcaggctga aatcgaactg gcattccatt
     4081 cgccggaaac agtcggaagc tggcatgcac gctggagtga ccgggtggca gagcatgacc
     4141 tggaaaccct gttctggcag tggggcgagc gttttccgtc actggccgga atggaacgct
     4201 ggcagtggca ggatatgcca ttctggcagg tcatcgcaga ggctggtatg gcggcaaggg
     4261 tggtcggtca tgctgttcgt gagatggagc ggtggatggt gccgaacaaa ctccgggagg
     4321 cagcgtgatg cggcaacaat cacacggatt tcccgtgaac ggtctgaatg agcggattat
     4381 tttcagggaa agtgagtgtg gtcagcgtgc aggtatatgg gctatgatgt gcccggcgct
     4441 tgaggctttc tgcctcatga cgtgaaggtg gtttgttgcc gtgttgtgtg gcagaaagaa
     4501 gatagccccg tagtaagtta attttctgta ctgcacccat tttgttggac gatgaaatgg
     4561 aatagcccct aatatgtcaa agccaaaata cccttttgaa aagcgccttg aagtcgtgaa
     4621 tcactacttc acaactgatg atggttacag gatcatctcg gcacgttttg gtgtcccccg
     4681 aacccaggtc aggacatggg ttgccctcta tgaaaaacat ggagaaaaag gtttaattcc
     4741 caaacctaaa ggcgttagtg ctgatccaga gttgcgtatt aaggtcgtga aagctgtgat
     4801 cgagcagcac atgtccctta atcaggctgc tgctcacttt atgcttgctg gtagtggttc
     4861 tgtagccagg tggctgaagg tctatgaaga gcgcggagaa gctggtttac gcgcgctcaa
     4921 gattggcacc aaaagaaaca ttgcaatatc agttgatcca gaaaaagcgg catcagcatt
     4981 ggagctgtca aaagaccgac gcattgagga tcttgaaagg caagttcgat ttcttgaaac
     5041 gcggcttatg tatctaaaaa agctgaaagc cttagctcat cccacgaaaa agtgaaagta
     5101 ctcaacgagc taaggcagtt ttatcctctt gatgagcttc tcagggctgc ggagataccg
     5161 cgcagtacgt tttattatca tctaaaggct ctcagcaagc ctgacaagta tgcggacgtt
     5221 aaaaagcgta ttagtgagat ttatcacgag aatagaggcc gatacggata ccgtagggta
     5281 acgctgtctc ttcatcgaga aggaaaacag attaaccata aagctgttca gcgcctgatg
     5341 ggaaccctct cacttaaagc agcgattaag gtcaagcgat accgctctta cagaggagag
     5401 gtagggcaaa ccgcccctaa tgttctccaa agagatttca aggctacgcg gccaaacgag
     5461 aagtgggtta ccgatgttac tgaatttgca gtcaatgggc gcaagctgta tttgtctcca
     5521 gtaatagatc tcttcaacaa cgaagttatt tcttacagcc tttcggaaag accagtgatg
     5581 aacatggttg agaatatgct cgatcaggca ttcaaaaagc ttaatcctca cgagcatcct
     5641 gttctgcact ctgaccaggg atggcagtat cgtatgagaa gatatcaaaa tatccttaaa
     5701 gaacatggta ttaaacaaag catgtccaga aaaggcaatt gtctggataa tgctgtggtg
     5761 gagtgtttct ttggaacctt aaagtcggag tgtttttatc ttgatgagtt cagtaatata
     5821 agcgaactga aggatgctgt tacggaatat attgaatact acaacagcag aagaattagc
     5881 ctgaaattaa aaggtctgac tccaattgaa tatcggaatc agacctatat gcctcgtgtt
     5941 taactgtcca actttttggg gtcagtacat tcattaacca ccacgaggca tccctatgtc
     6001 tagtccacat caggatagcc tcttaccgcg ctttgcgcaa ggagaagaag gccatgaaac
     6061 taccacgaag ttcccttgtc tggtgtgtgt tgatcgtgtg tctcacactg ttgatattca
     6121 cttatctgac acgaaaatcg ctgtgcgaga ttcgttacag agacggacac agggaggtgg
     6181 cggctttcat ggcttacgaa tccggtaagt agcaacctgg aggcgggcgc aggcccgcct
     6241 tttcaggact gatgctggtc tgactactga agcgccttta taaaggggct gctggttcgc
     6301 cggtagcccc tttctccttg ctgatgttgt acgggcatga acaacctgac ttcaggaagg
     6361 tcgtttccct tcaggaacgg ggatgaacgc gcgcctgcgg cgcccgttct tttcccccgc
     6421 cttctctggt tatggcctgt cagaaatcac atcaccttcc ctgtgattat tctctttttc
     6481 ttcctgctct gattctgact actgcagtgt tgtcctgtct gccgggcgca ccttcggctc
     6541 ccggctgcct ggcggcatcc ggtcgtcagg cccgactccg acaggcagct gctggcgcac
     6601 ctgccagtct ccgccgaacc cgctggcgcg gtttcgggct gtcgctacgg tccgatgcgt
     6661 gaaaacccc
//
LOCUS       DACADF010000211         6612 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.213, whole genome shotgun sequence.
ACCESSION   DACADF010000211 DACADF010000000
VERSION     DACADF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.213"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..189
                     /locus_tag="ISB30_004443"
     CDS             <1..189
                     /locus_tag="ISB30_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1992005.1"
                     /translation="ERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQ
                     LSIARSTVYKILEDERAS"
     gene            312..1292
                     /locus_tag="ISB30_004444"
     CDS             312..1292
                     /locus_tag="ISB30_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481-like element ISKpn27 family transposase"
                     /protein_id="HAP1992006.1"
                     /translation="MTQALHSQARTTHLIREEIRNSTLPQAELARMYNVTRQTIRKWQ
                     NRESPEDKSHAPNKMYTTLTPEQELIVVELRKTLLLPTDDLLAVTREFINPAVSRAGL
                     GRCLRRHGVSDLRNLVEQEGTAPATKKTFKDYEPGFVHIDIKYLPQMPDETARRYLFV
                     AIDRATRWVFIELYADQTDGSSGDFLNKVQQACPVKIVKLLTDNGSQFTDRFTAGGKK
                     KEPSGTHVFDRLCKQLGIEHRLIPPRHPQTNGMVERFNGRISDIVNQTRFGSAAELES
                     TLRNYVKIYNHSIPQRALQHKTPVQALKEWHEKRPELFRKRVYNQPGLDI"
     gene            complement(1405..1551)
                     /locus_tag="ISB30_004445"
     CDS             complement(1405..1551)
                     /locus_tag="ISB30_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992007.1"
                     /translation="MKVELGVAAGCNQAATAGIRGLTLSGTKTHVKKSFFNIIEAFIR
                     VIVS"
     gene            1568..2449
                     /locus_tag="ISB30_004446"
     CDS             1568..2449
                     /locus_tag="ISB30_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="HAP1992008.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSI
                     GVYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALV
                     PWSPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFR
                     LDRWELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNH
                     RIRAAVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAV
                     IAAAARLALEGLGVNGQ"
     gene            complement(2699..3580)
                     /locus_tag="ISB30_004447"
                     /pseudo
     CDS             complement(2699..3580)
                     /locus_tag="ISB30_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
     gene            complement(3684..3980)
                     /locus_tag="ISB30_004448"
     CDS             complement(3684..3980)
                     /locus_tag="ISB30_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor protein KorC"
                     /protein_id="HAP1992009.1"
                     /translation="MIRPETLRPFAEDWQAPTADEIKEVLELIRQRKGLSKPLSGVDV
                     ADLVGLPGERGSGKGTRTFRRWVSKTNPSPIAYGAWSILAHLAGFGAIWDADRD"
     gene            complement(4026..4181)
                     /locus_tag="ISB30_004449"
     CDS             complement(4026..4181)
                     /locus_tag="ISB30_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992010.1"
                     /translation="MNTDIVSLAKHIGDALTARRPVHMPRMKMRELGLLLIELKAYRE
                     AAATVTH"
     gene            complement(4309..4734)
                     /locus_tag="ISB30_004450"
     CDS             complement(4309..4734)
                     /locus_tag="ISB30_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP1992011.1"
                     /translation="MMQTELNPLICSLVATPRRMAAMPRYVGRFYVVFESMLYQQMKG
                     LCREYRGAYWLMWELSNGGFYMAPGRRDEMLNIEAMNYFSGQMSADAAGITACLYLYS
                     HLSFHTEGADQERFSRLYHSLRDWACEHDEKEAILAAID"
     gene            complement(4845..5123)
                     /locus_tag="ISB30_004451"
     CDS             complement(4845..5123)
                     /locus_tag="ISB30_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992012.1"
                     /translation="MIHTANRTFHQLYREWIRERREHMHNVLTWERDRYGARLVGLFY
                     RYCKVANPFPRCTLNTRINYRAHAVNLPDWPARSLELNKMWLSWREKK"
     gene            complement(5107..5502)
                     /locus_tag="ISB30_004452"
     CDS             complement(5107..5502)
                     /locus_tag="ISB30_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992013.1"
                     /translation="MANLPGTGSSSTGWPSSDLDARRKSTRWPTYQAPAAARPGGQVQ
                     IWTPEGNQPGGQLTRHRQQLDQVAHFVRTKSGDAMRNLGGNPPRLRDFFNVERAFSCH
                     SRPFWPIILIKKTGHPPRSIPENSDDPHS"
     gene            complement(5657..6217)
                     /locus_tag="ISB30_004453"
     CDS             complement(5657..6217)
                     /locus_tag="ISB30_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP1992014.1"
                     /translation="MRHLFWRLNFLLLSGATNSAQMYHEAAALSSELDTRWSYRSKEL
                     MTLYSKAKSYEAGERVEFGGKSFAPLYTPKNDTLINLFQISQQEQEQLKTIISTDEAQ
                     KRRRERDRLRDEERRRAAGQLEREAYEANSLSRQKPWEAMGMSRAKWYRLGKPSPQQN
                     SETSPSPITNGEASAVALQAARCKAV"
BASE COUNT         1418 a         1937 c         1777 g         1480 t
ORIGIN      
        1 gagcgcacga atgagggccg acaggaagca aagctgaaag gaatcaaatt tggccgcagg
       61 cgtaccgtgg acaggaacgt cgtgctgacg cttcatcaga agggcactgg tgcaacggaa
      121 attgctcatc agctcagtat tgcccgctcc acggtttata aaattcttga agacgaaagg
      181 gcctcgtgat acgcctattt ttataggtgt ctggactcgt gggatcatgt acccatgcgt
      241 agctggccgc tcttcaaggc cagacacgtt ttgcgtacac tgttccaatc cgactcttca
      301 ctggcaactc gatgacccag gcactgcaca gccaagcccg tactacccac ctgatccgtg
      361 aggaaatcag gaactcgacg ctcccgcagg ccgaactggc caggatgtac aacgtcaccc
      421 gccaaaccat ccgaaagtgg caaaaccgcg agtctcctga agacaagtcg catgcgccga
      481 acaagatgta cacgacgctc acgcccgagc aggagctcat cgtggtggag ctgcgcaaga
      541 cgttgctgct gcccacggac gacctgctgg cggtcacgcg cgagttcatc aatccagccg
      601 tctcgcgtgc cggcctggga cgttgcctgc gccgccacgg tgtctcggat ctacgtaacc
      661 tggtcgagca ggaaggcact gcgcccgcca cgaaaaagac cttcaaggac tacgagccgg
      721 gctttgtgca catcgacatc aagtacctgc cgcagatgcc cgacgagacg gcaaggcgct
      781 atctcttcgt tgccatcgac cgtgctacgc gctgggtctt catcgagctc tatgccgacc
      841 agaccgatgg cagcagtggc gacttcctca acaaagtcca gcaagcctgt cccgtcaaga
      901 tcgtcaagct tctgaccgac aacggcagcc agttcaccga ccgcttcacg gctggcggca
      961 agaagaagga acccagcggc acacacgtgt tcgaccgcct gtgcaagcag ctcggcatcg
     1021 agcaccggct catcccgcct cgtcatccgc agaccaacgg catggtggag cgcttcaacg
     1081 gtcgtatcag cgacatcgtc aaccagaccc gttttggttc agctgccgaa ctggaatcga
     1141 cgctgcgcaa ttacgtcaag atctacaacc acagcattcc gcaacgcgcg ctccaacaca
     1201 aaacacccgt tcaggcgctc aaggaatggc atgaaaaacg ccctgaattg ttcaggaaac
     1261 gcgtgtataa ccagccgggt cttgacatat aggttaatgt catgataata atggtttctt
     1321 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1381 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1441 attgaaaaat gacttcttaa cgtgagtttt cgttccactg agcgtcagac ccctaatccc
     1501 agctgtagcg gcctgattac atccggccgc tacacctagc tccaccttca aacaaggaat
     1561 atcgttgatg tcactgtatc gccgtctagt tctgctgtct tgtctctcat ggccgctggc
     1621 tggcttttct gccaccgcgc tgaccaacct cgtcgcggaa ccattcgcta aactcgaaca
     1681 ggactttggc ggctccatcg gtgtgtacgc gatggatacc ggctcaggcg caactgtaag
     1741 ttaccgcgct gaggagcgct tcccactgtg cagctcattc aagggctttc ttgctgccgc
     1801 tgtgctggct cgcagccagc agcaggccgg cttgctggac acacccatcc gttacggcaa
     1861 aaatgcgctg gttccgtggt cacccatctc ggaaaaatat ctgacaacag gcatgacggt
     1921 ggcggagctg tccgcggccg ccgtgcaata cagtgataac gccgccgcca atttgttgct
     1981 gaaggagttg ggcggcccgg ccgggctgac ggccttcatg cgctctatcg gcgataccac
     2041 gttccgtctg gaccgctggg agctggagct gaactccgcc atcccaggcg atgcgcgcga
     2101 tacctcatcg ccgcgcgccg tgacggaaag cttacaaaaa ctgacactgg gctctgcact
     2161 ggctgcgccg cagcggcagc agtttgttga ttggctaaag ggaaacacga ccggcaacca
     2221 ccgcatccgc gcggcggtgc cggcagactg ggcagtcgga gacaaaaccg gaacctgcgg
     2281 agtgtatggc acggcaaatg actatgccgt cgtctggccc actgggcgcg cacctattgt
     2341 gttggccgtc tacacccggg cgcctaacaa ggatgacaag cacagcgagg ccgtcatcgc
     2401 cgctgcggct agactcgcgc tcgagggatt gggcgtcaac gggcagtaag gctctgaaaa
     2461 tcatctattg gcccaccacc gccgcccttg cgggcggcat ggattaccaa ccactgtcac
     2521 atttaggcta ggagtctgcg cggcagagcc gtgtgaccgg ttttctgtag agcactgacg
     2581 atggcggcgg cgctctctgc aattggcaag gcgtcggcgc caaggatacc aatcttgcgg
     2641 cgcgcggcgt gttatgacga ctggggtgca tttgagccgc cccatttaac cttcgccctc
     2701 acagatacgc cattcgcctc agatttagcg ccatgcagac gagcttccac tcggcttgca
     2761 ccttgtccag gcccctcatg ctgaactgac gcaatcccat caccgccttg atccaaccat
     2821 tcggagcctc cacgatcgac ttgcgccggc ggtaagctgc atcgccttgc tccgttttca
     2881 atttcgccgc aatcgccgcc gtatgcggat gggtcttggc attgaccttg gcatcttcac
     2941 gtccctcgcg gccgagggca acgatgacat cgccgtggtg atcggcgacc tttgccagaa
     3001 cagcctcact acggaatccc gcatccgcca gcgtctgggc cggcatttct ccggtgttgg
     3061 cctgaactgc tgccagcatg cccagcagcg cctgactgtc cgcggcgcag ttggtcaact
     3121 ccgccgccac gatgatctgg tgctcggcat cgaccgctgt gtacccgttg tagctctgct
     3181 cggagccacc accggcgtgt ttcatgatcc ggctgtccgg atcggtgaag ctttcctgat
     3241 cacggtcatc cggcacacca aactcgcgtt tgtacgagcc accgcccttg tccgagccat
     3301 ccggatggcg aggccggcgg ccatcgtctt cgctgcgccc ccgggcctgg tccgcttcac
     3361 gctggcgcgc ttccaggcgc gcctttgccg cctggatcgc ctccaggcgc ttctcgcggc
     3421 gagaaatctc ggcaggaatg tccagctccg gctcgttacg ctcctggtcg tcggtagcct
     3481 tggcgcgatc aagcagcgcc ttgatctcgc aatgcaattc gtcctcggcc ggcttcatgc
     3541 gcttatagct catcgccttg tggtcgccat gtttggcggc ttcgtattca ggttctttag
     3601 agagatcctt tagatcgccg ttcctcacta ctgccgggaa gtctccccag ggagcacgtt
     3661 cggccttatg ttcggtcatg gtttcaatcc cggtcagcgt cccatatagc cccgaaacct
     3721 gccaggtggg ccaagataga ccaggcacca taagcaatag ggctagggtt ggttttgctc
     3781 acccaacgcc tgaaggttcg ggttcccttt ccgctgcctc tctcaccagg caaaccaacg
     3841 agatcagcga catcaacgcc actgagaggc ttactaagcc cttttcgttg cctaatcagc
     3901 tccaacacct ctttgatttc atcggcggtt ggagcctgcc aatcttcagc gaacggccta
     3961 agcgtttcag gtctaatcat gcgataaatc ctcaaatagc cccggtcaat gccggggctt
     4021 ttttatcaat gggtaacggt cgcggcggcc tcccgatacg ccttcagttc aatcaagagc
     4081 aagccaagct cccgcatctt catgcgcggc atatgaacgg ggcggcgggc cgtcagagcg
     4141 tcgccaatgt gttttgcaag tgatacaata tccgtattca tcgcgtttat cctcaaattg
     4201 accggtgaaa gcccctgtga ttggttgcag ccataccagg ggcaccattt ctttttctac
     4261 ctatgcacct cctttaagag ccccggccat caccggggct ttctgttgct agtctattgc
     4321 ggccaagatg gcctcttttt cgtcatgctc acaagcccaa tcccgcaagc tgtgatacag
     4381 ccttgaaaac cgctcctggt cagcgccttc tgtgtgaaat gacagatggc tatagaggta
     4441 gaggcaagcg gtaatcccgg cagcatcggc gctcatttgg ccactaaagt agttcatggc
     4501 ctcgatgttc agcatttcat cacgacgccc aggagccata taaaagccgc cgttcgacag
     4561 ctcccacata agccaataag ccccgcgata ctcgcggcag agccctttca tttgctggta
     4621 aagcatcgac tcgaaaacca catagaaacg gcctacatag cggggcattg cagccatacg
     4681 gcgaggggtg gcgaccaggg agcagatcag gggattaagt tctgtttgca tcatcggagt
     4741 tctccggtat tgaccgtggc gggtgcccgg ccttctgaga tatataatag gccattttgg
     4801 cctattgtgt caagcgggta caataaaaaa taggccaaat cggcctactt tttttctctc
     4861 caactgagcc acattttatt cagctccagg ctgcgggctg gccaatctgg cagattcacc
     4921 gcatgggcgc ggtagttgat ccgagtattc agagtgcaac ggggaaaggg gttggccacc
     4981 ttgcagtaac gataaaacag accgacgagg cgagcaccgt aacgatcacg ctcccaggtc
     5041 aaaacgttgt gcatatgctc gcggcgttcg cgtatccatt ctcgatagag ttggtgaaag
     5101 gtgcggttag ctgtgtggat catcggagtt ctccggtatt gaccgtggcg ggtgcccggt
     5161 cttcttaatt aagattatag gccaaaatgg cctactatgg caagaaaaag cgcgctctac
     5221 gttaaaaaaa tcacgtagtc gcgggggatt gccgccaaga tttcgcatag cgtcgcctga
     5281 tttcgtacgt acgaaatggg ccacctggtc gagctgctgc cgatgcctgg tgagttggcc
     5341 acctggttga tttccttctg gcgtccagat ctgaacttgg ccacctggtc gagctgctgc
     5401 cggtgcctgg taagttggcc acctggttga tttccttctg gcgtccagat ctgaacttgg
     5461 ccacccggtc gagctgctgc cggtgcctgg taagttggcc acctggtcga actgctgccg
     5521 gatctaaagg tggaatatta agcaggtagt gatagactga gatccagcat ggctatacgc
     5581 ctgctaaccc ctcaaaagaa agcagcctct agggctgcca tccgttacga aatctcactc
     5641 actttgaaat agccatctaa acggctttac acctggccgc ctgaagggca accgctgagg
     5701 cttcgccatt agtaataggg gagggacttg tctcactgtt ttgttgaggt gaaggcttgc
     5761 ccaggcgata ccatttagcc cggctcatgc ccatagcttc ccagggcttt tgcctgctca
     5821 atgagttggc ttcataggct tcacgctcaa gctggccagc ggcgcggcga cgttcttcat
     5881 cacgcagtct atcgcgctct ctgcggcgct tctgagcctc gtcagtgctg ataatcgtct
     5941 tcagttgctc ttgttcttgc tgtgagatct ggaataggtt gatcagcgtg tcgtttttgg
     6001 gcgtgtatag cggtgcaaac gactttccgc cgaactcgac acgttcccca gcctcatagg
     6061 atttcgcctt gctgtagagc gtcataagct ctttggagcg gtaagaccaa cgagtatcca
     6121 gctcggagga aagagcggcg gcctcatggt acatctgggc gctgtttgtt gccccggaga
     6181 gaaggagaaa gttaagacgc cagaaaagat gcctcattcg ctcgccttcg ttgattccac
     6241 ctctgagctg gcccaacttg cgtaggtctt ccagggggaa ccgcagaatt cggaaaaaat
     6301 cgtacgctaa gctaaggaag gtctgaaaaa gcccccgctc gagaaatgca tcccagcaac
     6361 catgcgggtt acagagcggt gatttcgcga aacatgggct ttttcagagc ttccttagcg
     6421 ttacgataaa gctagcatgg aaacgatagg tgcaagcaag ttaagggttg catcgcgcat
     6481 gtcaatctag gctataccct aacttggcgt cagaccatcc ggcgctaaat cgtcagaata
     6541 gagttgcctt ccgaattgat tgacatacgc cgtcaagggt catagatttc ttcctgacac
     6601 atttccctca gg
//
LOCUS       DACADF010000212         6512 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.214, whole genome shotgun sequence.
ACCESSION   DACADF010000212 DACADF010000000
VERSION     DACADF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.214"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            121..1455
                     /locus_tag="ISB30_004454"
     CDS             121..1455
                     /locus_tag="ISB30_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP1992015.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSISSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1508..1960
                     /locus_tag="ISB30_004455"
     CDS             1508..1960
                     /locus_tag="ISB30_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAP1992016.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2076..2399)
                     /locus_tag="ISB30_004456"
     CDS             complement(2076..2399)
                     /locus_tag="ISB30_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAP1992017.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSRVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(2383..2736)
                     /locus_tag="ISB30_004457"
     CDS             complement(2383..2736)
                     /locus_tag="ISB30_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP1992018.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADVLGGGVFKKR
                     LHRNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            2970..4160
                     /gene="nepI"
                     /locus_tag="ISB30_004458"
     CDS             2970..4160
                     /gene="nepI"
                     /locus_tag="ISB30_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAP1992019.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4201..4494)
                     /locus_tag="ISB30_004459"
     CDS             complement(4201..4494)
                     /locus_tag="ISB30_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992020.1"
                     /translation="MKPTMLLMITIFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            4716..5534
                     /gene="nlpA"
                     /locus_tag="ISB30_004460"
     CDS             4716..5534
                     /gene="nlpA"
                     /locus_tag="ISB30_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAP1992021.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(5538..6461)
                     /locus_tag="ISB30_004461"
     CDS             complement(5538..6461)
                     /locus_tag="ISB30_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP1992022.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
BASE COUNT         1559 a         1473 c         1634 g         1846 t
ORIGIN      
        1 cgcgagatta ttaaagaata tccattaatg tgcaattgaa atgtgataat tatcactgaa
       61 ccgttgttgt catttgtttt acaaaagcaa gggattaaat ctcctccaat ggacaaaaaa
      121 atgaataatg acaataccga ttacgtgagt aatgaatcag ggacgctttc gcgattattt
      181 aaactacctc agcatgggac caccgtccgc acagaattga ttgcggggat gaccactttt
      241 ttaaccatgg tgtacatcgt ttttgtgaac ccgcaaatcc tcggcgcggc acaaatggac
      301 ccaaaagtgg tgtttgttac cacctgtttg attgccggta tcggcagtat tgcgatgggg
      361 gtatttgcta acttacccgt ggcactggct ccggcaatgg gcctgaacgc cttctttgcc
      421 ttcgtggtcg tgggggcgat gggtatctcc tggcagaccg ggatgggcgc gatattctgg
      481 ggcgcagttg ggctattttt gctcacgctg tttcgtatcc ggtactggat gatctccaac
      541 attccattaa gtttacgtat tggtatcacc agcggaatcg gattatttat cgccttaatg
      601 ggattaaaaa atactggcgt tattgtcgcc aataaagaca ccctggtgat gattggcgat
      661 ttaagttctc acggcgtgtt gttaggtatt ttagggtttt ttattataac tgtgttgtca
      721 tcacgtcatt ttcatgccgc ggtgcttgtt tctattgtgg tgacgtcttg ctgtggatta
      781 tttttcggtg atgttcattt tagcggtgtc tattccattc cgcctgatat tagcggcgtc
      841 attggtgaag tggatttgag cggtgcgtta tcacttgaac tcgccggtat cattttctcc
      901 tttatgctga tcaacttatt tgattcatca gggacattaa ttggtgtgac tgataaagct
      961 ggcttaatag atagtaacgg taaattcccc aatatgaata aggcgctgta tgttgatagc
     1021 atcagttcgg tggcgggtgc gtttatcggt acctcgtctg ttaccgccta tattgaaagt
     1081 acttcaggtg tggctgtcgg tggtcgcacg gggctgactg cggttgttgt cggcgttatg
     1141 tttctgctgg ttatgttctt ctcaccgctg gtggcgatgg ttcctcctta cgcaaccgcc
     1201 ggagcgttaa tctttgttgg cgtattgatg acatcgagcc tggcacgcgt taactgggat
     1261 gattttaccg aatcggtgcc tgcgtttatt accacggtga tgatgccctt tacgttctcg
     1321 atcaccgaag ggattgcact aggctttatg tcgtactgca tcatgaaagt gtgcaccggg
     1381 cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag ctctgtttgc actgaagatt
     1441 attctggtgg attagtaact ggcgagggtt tccagatatc atgagttctg attacgcagg
     1501 agaactcatg atctggataa tgctcgccac gctggcggta gtgtttgtgg ttggttttcg
     1561 ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc agcgatcggc tgaacatcga
     1621 tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag tcagccggtg acgaattttt
     1681 acgttatttg catcgcccgg atgagtcgca cctgcaaaac gccgcgcagg tgttgctcat
     1741 ctggcaaatt gtcattgtcg atggtagcga acagaacctg ctgcaatggc atcggatttt
     1801 acaaaaagcc cgccttgccg cgccgattac cgatgctcag gtcagactgg cgctgggttt
     1861 tctgcgcgaa accgaacctg aaatgcagga tatcaatgct tttcagatgc gctataacgc
     1921 gttctttcag cctgccgagg gcgttcactg gctgcattaa aaccgcttcg tcttatcagc
     1981 ctacagaccg cgcctgacct ggtgggtgga taaggcgtgt acgtcgcatc cggtatcgga
     2041 attgacagta ggtgaacgca ttatctacgt tctatttaca gcagcacctc cagcccgtgt
     2101 ttttgcacaa tgctgagcag ttttaatgaa atgccattgg ggcgtttagc gccactttcc
     2161 catttttgta cagttgaaac gctggtattg agatagtgag cgaagacggg ctgactcact
     2221 tttaattttt cccgcagtgc ttttatctca acaggttgta agtcttgcac gctggtcagg
     2281 caggctttgt caaaagtacg catcgtttcg gcagaaatag catcgactct ggacaatcca
     2341 gccgctgcgc tgtgaatagc ttcaaatgca ggacttttaa atttagtttt tgcagtcata
     2401 gcagatctct gtcagttctt tagtgttaat cattgctgtc agttgggctt tcgtcaggaa
     2461 ggcataatgc tttgcaagtt cacgaaaccc agccagttcc tgactattaa tgttcgacat
     2521 atcctgtttt gcatataaaa acgtgtaaaa ccaattgctt ccccctttcg ccagaataat
     2581 tgcacgctca cgatttcggt gaaggcgctt tttaaaaacg ccaccaccaa gtacatcagc
     2641 tttaccttgt tccgtttcta aaatggcccg gcacaactct tcatctgtta ttgcatgaga
     2701 cctggcttcc cttgcaaacc atttggtttt aaacacgcgc atatcgttga gatcctgtta
     2761 ttaaagtata tcaccaagtg caatacttta tcccttattc ccaacctaat cgcgaaaatg
     2821 catatcacct tgccctatga tttttatttc ttcgagcagc ctcgaacttt tcccacaaca
     2881 ccgtaaaagt tgcaaaaacg ttaaatacgt cacacatttc aatgttaaag tggtcggctt
     2941 tccctctgaa acatgccacg ggtaacacca tgagtgaatt tattgctgaa aaccgcggcg
     3001 cggatgccat cacccgaccg aactggtcag ccgttttctc ggtggcattt tgcgttgcct
     3061 gtcttattat cgttgagttt ttgcccgtca gtttgttgac gccaatggcc caggatttag
     3121 gcatttcgga aggggttgcc gggcaatcgg tgaccgtaac cgcttttgtg gcgatgtttg
     3181 ccagtttgtt tattacccag accattcagg ccactgaccg ccgctacgtt gtcattctgt
     3241 tcgccgtttt gctgacgctt tcctgtttgc tggtttcctt tgccaactca ttcagtctgt
     3301 tgttaatcgg tcgcgcctgt ctggggctgg cgctgggcgg attctgggcg atgtcggcgt
     3361 cgctgaccat gcgtctggta ccgccgcgta cggtgccgaa ggcgctgtcg gtgatcttcg
     3421 gcgcggtttc tattgcgctg gtgattgccg cgccgttggg cagtttttta ggcgagctta
     3481 tcggttggcg caatgtcttt aatgcggcgg cggtgatggg cgtgctgtgt attttctgga
     3541 ttatcaaatc actgccttcg ctgccaggcg aaccttcgca tcagaaacaa aatactttcc
     3601 gcttattaca acgtccggga gtgatggccg ggatgatcgc catctttatg tcctttgccg
     3661 gacagtttgc ttttttcact tatattcgcc cagtgtatat gaatctggcg ggattcggtg
     3721 ttgatggctt aaccctggtg ctgctgagtt ttggtatcgc cagctttgtc gggacatcgc
     3781 tttcgtcgtt tattctcaaa cgttcggtga aactggcctt agcgggcgca ccgtttgtgc
     3841 tcgccctgag cgcgctggta ctgacgctgt ggggaagcga taaaattgtt gctactggtg
     3901 tggcgattat ctgggggctg acctttgctt taattcccgt tggctggtca acgtggatca
     3961 ctcgctcact ggccgatcag gcagaaaaag ccggatctat tcaggtggcc gttattcagc
     4021 ttgccaatac ctgcggtgcg gcaatcggcg gttatgcgtt ggataacatt ggtctgactt
     4081 cgccgctgat gttgtccggc acattgatgt tgctgactgc attgttggtg actgcaaagg
     4141 tgaaaatgaa gaaatcctga tatcaggtaa agaagcgttg gtcagtggtt gttgccgttc
     4201 ttacatatcc gggcattcta ccttacccaa cgcttcccaa taattgctct gattattacg
     4261 ttccatcgct acgatggctt cgcgtatgtg ttgtttattg ctttcatcag acaacattaa
     4321 cttttcccac gcttcatctg ataacgatgc ctgcggggta caaattttac gtaaatcttg
     4381 caataccgtg gttttctctt ttgccgactg tagggaactg aatggcgact cggcctgaga
     4441 aatggctgga aaaatcagga atatggtaat catgagtagc atcgttggct tcatagcggc
     4501 aaccttctgc aaatgtgcat cgaatgaagc ataagtgtag tttgcttttg ccgctctcat
     4561 catgctgtac gaaaggtgtt tgcgtggtca gtaagaagtg ccagacttta tattccactt
     4621 ttattccttt ttattcttat cgatagcgtt tcgtttttta aactgcagcg accttaccgc
     4681 tatagtcagg taatcattaa taaaaggata aaaaaatgaa actgacaaca catcatctac
     4741 gggcgggggc cgcattatta ctggcaggag ttctgctggc aggttgcgac cagagcagta
     4801 gcgatgaaaa gcatattaaa gttggcgtta ttaatggcgc agaacaagat gtcgcggaag
     4861 tcgctaaaaa ggtagcaaaa gagaaatatg gcctcgatgt tgaactggta ggatttagcg
     4921 gttcgttgct accgaacgat gcgacaaatc atggcgagct tgatgcgaac gtcttccagc
     4981 atcgaccctt tcttgaacaa gataatcagg cgcatggtta taaactggta gcggtgggaa
     5041 atacgtttgt cttccccatg gcgggttatt ccaaaaaaat caaaaccgtg gcgcaaataa
     5101 aagaaggcgc gacagtggcg atccccaacg acccgactaa ccttggtcgt gcgcttttac
     5161 tgctgcaaaa agagaagcta attactctga aagagggaaa agggttatta cctaccgcgt
     5221 tggatataac cgataatcca cgccatttgc agataatgga gctggaaggg gcacagttgc
     5281 cacgtgtact ggacgacccg aaagtggatg tagcgattat cagcaccact tacatccagc
     5341 aaacaggact ttctccggtt aacgacagcg tatttattga agataaaaat tcgccgtatg
     5401 tgaatattct ggtggcacgg gaagataata agaatgcaga aaacgtgaag gaatttctgc
     5461 aatcttatca atcacccgaa gtcgctaaag cggcagaaac catctttaac ggaggcgcgg
     5521 tgcctggctg gtaattctca ctcatgccgc gccggacgat ttatttttct ggcgcggcgg
     5581 cgggaatcca ttgaaatcaa aatcaccgat gacagaatca gcagcgttcc cagccagtcc
     5641 ggcaatgtga aggtgatccc cagcaacagc aaagagagca gtgcgctact taatggttct
     5701 gcacagctta aaatgctggc ttttggacca ccaattaatt gtgctccttt caggtacaga
     5761 ctaaatgtca gggacgtacc aatgaccacc aaataaaaaa acgccagaat caaactgccg
     5821 ttaacgacaa agtttgtccc ttgtctggca taaaagggca gcaggatcag accgccaatg
     5881 agcatactcc agccgacgac cggtaacgtg ccatagcgag caatcagcgt tgagggatag
     5941 gtggtataga atgcagcagc aaatgccgag gcaatgcccc agaataacgc ggcaggagag
     6001 atcgataatg acgtcggatt accgtgtgtc accaataaaa aagtcccgat cagcgatgtc
     6061 aaaatagcgc aaaaaaccag gatgccgggg cgggatttac gcaccagtga gaaccaggcg
     6121 acgataatag tcggtgagag gaattgcagc accgttgccg tggctgcgtt cgatttttcg
     6181 atggttagca aaaaagtgag ctgtacggtt agcgcgccaa ccacggaaaa aatcagtagg
     6241 ctaatggcat ctttgtgatt gttaataata gaaaagattt tatcaccatg aacaaatgat
     6301 agcgtcagca gaatcaatcc ggcgaatatc aaacgcgtca tagtcaaaaa ctgcgacgac
     6361 atctggcttt gctccatgat gtattgcgcg cagacccctg aacttcccca caacacggcg
     6421 gcaatcagaa cgttcagcat cccctttctg gtggaaccca tttttccctc tgcatactgt
     6481 tttgtctttt catcaatata cgttaaggat ac
//
LOCUS       DACADF010000213         6508 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.215, whole genome shotgun sequence.
ACCESSION   DACADF010000213 DACADF010000000
VERSION     DACADF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.215"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(268..1917)
                     /gene="pgi"
                     /locus_tag="ISB30_004462"
     CDS             complement(268..1917)
                     /gene="pgi"
                     /locus_tag="ISB30_004462"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP1992023.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2442..3791
                     /gene="lysC"
                     /locus_tag="ISB30_004463"
     CDS             2442..3791
                     /gene="lysC"
                     /locus_tag="ISB30_004463"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAP1992024.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4044..4316
                     /locus_tag="ISB30_004464"
     CDS             4044..4316
                     /locus_tag="ISB30_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAP1992025.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4317..5189)
                     /gene="rluF"
                     /locus_tag="ISB30_004465"
     CDS             complement(4317..5189)
                     /gene="rluF"
                     /locus_tag="ISB30_004465"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAP1992026.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKSGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5537..6484
                     /locus_tag="ISB30_004466"
     CDS             5537..6484
                     /locus_tag="ISB30_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAP1992027.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFGLKDVVVVSGNDEDEDTQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
BASE COUNT         1598 a         1616 c         1663 g         1631 t
ORIGIN      
        1 cacgattaaa aatttatttt aggattaacc tcacggtatg attttcgtta aatgacagac
       61 aagaggattt atctgataaa acaatgccga cttatagtcg gcattgtttt taaggctatt
      121 taatcaatcg tactatgtat taattgccga atacgatgta catatcggca tcgacctgta
      181 ggcctgataa gacgcgaccg cgtcgcatca ggcatcggtt gccggatgcg gcgtgaacgc
      241 cttatccggc ctacatatcg acaatgatta accgcgccac gctttatagc ggttaatcag
      301 accattggtc gagctatcgt ggctgctgat ttctttgtca tctttcagct ctggcagaat
      361 acggttcgcc agttgtttac ccagctccac gccccattgg tcgaaggtga agatgttcag
      421 gatcacgccc tgagtaaaga ttttgtgctc atacagcgca atcaacgccc ccaggctgaa
      481 cggagtgatt tcacgcagca ggatggagtt ggtcgggcgg ttaccttcga acactttgaa
      541 cggcaccacg tagtcaagcg ttgccggatc tttaccctga tcgcgatatt cctgctcaac
      601 cacttcgcgg gatttaccaa acgccagcgc ttcggtctga gcaaagaagt tagacagcag
      661 tttctggtga tgatcagaga gcgggttatg ggtgatagcc ggagcgatga aatcgcacgg
      721 taccattttg gttccctggt ggatcagctg atagaacgcg tgctgaccgt tcgtgccagg
      781 ttcaccccag ataatcgggc cagtctggta atccacaacc ttaccgttac ggtcaacata
      841 cttaccgttg gattccatat tgccctgctg gaagtacgcc gcgaaacggt gcatatactg
      901 gtcatacggc agaatcgctt cagtttccgc gccaaagaaa ttgttgtacc agatgccaat
      961 cagcgccagc agtacaggca ggtttttctc ggcaggcgtg gtggagaaat gcttgtccat
     1021 cgcgtgtgcg ccagaaagca gttcaacgaa gttatcaaag ccgatggaga gaacaatcga
     1081 caggccaatc gctgaccaca aagaataacg accgccgacc cagtcccaga attcgaacat
     1141 gttggcagta tcaataccaa actcgccaac ggctttggcg ttggtggaaa gcgccgcaaa
     1201 gtgtttcgca acgtgcttct catcacctgc cgctttcagg aaccagtcac gcgcgctatg
     1261 ggcgttggtc atggtttcct gagtggtgaa ggttttagat gctaccagga acagcgtggt
     1321 ttccgggttt acttttttca gcacttccgc gatgtgagtc ccatcgacgt tagaaacaaa
     1381 gtgcatgttc aggtggtttt tgtatggacg cagtgcttca gtcaccatgt acgggccgag
     1441 gtcagaaccg ccgatcccga tgttcactac gtcagtgatt gccttgccgg tatagccttt
     1501 ccactcaccg gaaataatcg cttccgagaa ggttttcatc ttctccagca ccgcgttgac
     1561 ttccggcata acatctttgc catcaaccag aatcggggta ttgctacggt tacgcagcgc
     1621 tacgtgcagt acggcgcggt tttcagtgcg gttgatcttc tcgccagaga acatcgactt
     1681 aatcgcgccc gccagatcgc actctttcgc cagatcttgt aatttcgcca gcgtctcttc
     1741 agtgatgcgg tttttggagt aatccaccag catctgatcg tcgaaggttg cggagaactt
     1801 agaaaaacga tcgccgtctt tagcaaaaag atcggcgatc gtaacgtctt tcatttcatc
     1861 gaagtgtttc tgtagtgcct gccaggcagc ggtctgcgtt ggattgatgt ttttcattag
     1921 caatactctt ctgattttga gaattgtgac tttggaagat tgtagcgcca gtcacagaaa
     1981 aatgtgatgg ttttagtgcc gttagcgtaa tgttgagtgt aaacccttag cgcagcgaag
     2041 catttattag ctgaactact gaccgccagg tgtggatgaa aaatccgcat gaccccaccg
     2101 ttgacaaccg ccccgctcac cctttattta taaatgtact acctgcgcta gcgcaggcca
     2161 gaagaggcgc gttgcccaag taacggtgtt ggaggagcca gtcctgtgat aacacctgag
     2221 ggggtgcatc gccgaggtga ttgaacggct ggctacgttc atcatcggct acaggggctg
     2281 aatcccctgg gttgtcacca gaagcgttcg cagtcgggcg tttcgcaagt ggtggagcac
     2341 ttctgggtga aaatagtagc gaagtatcgc tctgcgccca cccgtcttcc gctcttccct
     2401 tgtgccaagg ctgaaaatgg atcccctgac acgaggtagt tatgtctgaa attgttgtct
     2461 ccaaatttgg cggtaccagc gtagctgatt ttgacgccat gaaccgcagc gctgatattg
     2521 tgctttctga tgccaacgta cgtttagttg tcctctcggc ttctgctggt atcactaatc
     2581 tactggtcgc tttagctgaa ggactggaac ctggcgagcg attcgaaaaa ctcgacgcaa
     2641 tccgcaatat ccagtttgcc attctggaac gtctgcgtta cccgaacgtt atccgtgaag
     2701 agattgaacg cctgctggag aacattaccg ttctggcaga agcggcggcg ctggcaacgt
     2761 ctccggccct gacagatgaa ctggtcagcc acggcgagct gatgtcgacc ctgctgtttg
     2821 tcgagatcct gcgcgaacgc gatgttcagg cacagtggtt tgatgtacgt aaagtgatgc
     2881 gtaccaacga ccgatttggt cgtgcagagc cagatgtagc cgcgctggcg gaactggccg
     2941 cgctgcagct gctcccacgc ctcaatgaag gcttagtgat cacccaggga tttatcggca
     3001 gcgaaaataa aggtcgtaca acgacgcttg gccgtggagg cagcgattat acggcagcct
     3061 tgctggcgga ggctttacac gcatctcgtg ttgatatctg gaccgacgtc ccgggcatct
     3121 acaccaccga tccacgcgtg gtttctgcag caaaacgcat tgatgaaatc gcgtttgccg
     3181 aagcggcaga gatggcaact tttggtgcaa aagtactgca tccggcaacg ttgctacccg
     3241 cagtacgcag cgatatcccg gtctttgtcg gctccagcaa agacccacgc gcaggtggta
     3301 cgctggtgtg caataaaact gaaaatccgc cgctgttccg cgcgctggcg cttcgtcgca
     3361 atcagactct gctcactttg cacagcctga atatgctgca ttctcgcggt ttcctcgcgg
     3421 aagttttcgg catcctcgcg cggcataata tttcggtaga cttaatcacc acatcagaag
     3481 tgagcgtggc attaaccctt gataccaccg gttcaacctc cactggcgat acgttgctga
     3541 cgcaatccct gctgatggag ctttccgcac tgtgccgggt ggaagtggaa gagggtctgg
     3601 cgctggtcgc gttgattggc aatgacctgt caaaagcctg cggcgttggc aaagaggtat
     3661 tcggcgtact ggaaccgttc aacattcgca tgatttgtta cggcgcatcc agccataacc
     3721 tgtgcttcct ggtgcccggc gaagatgccg agcaggtggt gcagaagcta cattttaatt
     3781 tgtttgagta aacacagtat ggcccggaag acattatttc gggccgtatt gtttttcttg
     3841 tcattatgcc cataaataaa cgagcctgta ctctgttaac cagcgtcttt atcggagaat
     3901 aattgccttt aattttttat ccgcatctct aactaattat cgaaagagat aaatagttaa
     3961 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
     4021 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
     4081 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
     4141 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
     4201 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
     4261 atcctgaggc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctgattaa
     4321 cgccccttct ttttgcgccc cggcgaggta aaacgcttac cgttggacgc cgggcgaccg
     4381 cctttttccg ccgttttttc catcttaacg actggacgtt tgatgcctac cgttttcggt
     4441 ttggccttcg ctttaggttt tgcctcggaa gaggaatttt caatgagctt aaagagatcg
     4501 attaactcat cgtcggttaa atcgcgccac tcacccagcg gaatgccgct taagctgacg
     4561 ttcataatgc gcgtacgttc cagctttttc acttcgtaac caaagtgctc gcacatgcga
     4621 cgaatctgac ggttcagccc ctgcaccagg gtaatgcgga agacaaacgg tgcttctttt
     4681 ttgactttgc actttttagt taccgtcccg aggatcggca ctcccgcccc cataccacga
     4741 ataaattcat cggtaatcgg tttatcaacc gtcaccagat actctttctc atgatcgtta
     4801 cccgcacgga ggatcttatt cactagatcg ccgtgattgg tgaggaaaat cagcccctgg
     4861 gaatctttat ccaggcggcc aatcgggaac acgcgtttgc tgtggttaac gaaatcgaca
     4921 atgttatcgc gctcgccatc ttcggtggtg ctgacaatgc ctacgggctt gttcagggcg
     4981 ataagtacca aatcttcggc ttcccgaggc tcaatcaact gaccgtttac tttcacaacg
     5041 tcgccggatt tcacctgatc gccaatggtg gctcgcttgc cattaaggaa cacattgcct
     5101 tgctcgatat agcgatccgc ttcgcggcgt gagcaaattc cgctttcgct gatgtattta
     5161 tttaaacgga ctgatgagtc gggcagcata aattctcctg taaaacgcgg aatataccgt
     5221 acctcttagc ggggaaaaac agggttatgc ggcaacgtcg aaattaaatg tgatctgcca
     5281 cgccttttat cacaaaagtg tctccggaag tacgatgcaa atttgcctgg caaaataagg
     5341 ataatcaaca aattacgaat cgctgcacac ctgtgctgat gactactacg cagagaaaca
     5401 aatgtgcaag agaaagcgct atttcccccc atgcaaagag tgaaaaatcg ctttgaaagg
     5461 ccatcatgat ggggttatat cagatcaagc ggtgagaaag cgtatttgca caaatgtgca
     5521 gaaccaggag gcggcaatgg aaaacagtga cgatatccgt ttgattgtga agattgccca
     5581 actctattac gaacaggata tgacgcaggc gcaaattgcg cgcgaactgg gtatttaccg
     5641 caccaccatc agccgcttgc ttaaacgagg ccgcgatcag ggaattgtca ccatcgccat
     5701 caactatgac tacaacgaaa atctctggct ggagcagcaa gtgaagcaaa agtttggcct
     5761 gaaagacgtt gtggtggtgt caggaaatga tgaggatgaa gatactcaac tggcgatgat
     5821 ggggttacac ggcgcgcaac tgctggatcg cttgctggaa cctggtgata ttgttggttt
     5881 ttcctggggt cgcgcggtga gcgcactggt tgaaaacttg ccgcaggcgg ggcaatcgcg
     5941 gcagttaatt tgcgtgccga ttattggcgg cccgtccggt aaactcgaaa gccgctatca
     6001 cgtaaacaca ttaacctaca gcgcggcagc gaagctgaaa ggggaatcgc atctcgcgga
     6061 ttttccggct ctgctggata acccattaat tcgtaatggg atcatgcagt ctcagcactt
     6121 taaaaccatc tctgcctact gggataatct ggatgtcgcc ctggtgggaa ttggctcacc
     6181 ggccattcgc gacggcgcta actggcatgc gttttatggt ggtgaagaga gtgacgacct
     6241 gaatgcccgc caggttgctg gcgatatttg ctcgcgcttt tttgatattc acggcgcaat
     6301 ggttgaaacg aatatgagcg aaaaaacact ctctatcgaa atgaataaat taaagcaggc
     6361 acggtattcc attggcattg ccatgagcga agaaaaatac agcggaattg ttggtgcact
     6421 gcgtggaaaa tatattaatt gtctggtaac gaatagcagc acagctgaac tattactgaa
     6481 ataaaaatct tcccgatgtt taccgtaa
//
LOCUS       DACADF010000214         6493 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.216, whole genome shotgun sequence.
ACCESSION   DACADF010000214 DACADF010000000
VERSION     DACADF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.216"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..237)
                     /locus_tag="ISB30_004467"
     CDS             complement(<1..237)
                     /locus_tag="ISB30_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1992028.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFS"
     gene            450..1241
                     /locus_tag="ISB30_004468"
     CDS             450..1241
                     /locus_tag="ISB30_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HAP1992029.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1251..2093
                     /locus_tag="ISB30_004469"
     CDS             1251..2093
                     /locus_tag="ISB30_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP1992030.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQSEDLQQTARQLAASNMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAANRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            2103..2879
                     /locus_tag="ISB30_004470"
     CDS             2103..2879
                     /locus_tag="ISB30_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAP1992031.1"
                     /translation="MNDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTALRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQTANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            2889..4430
                     /locus_tag="ISB30_004471"
     CDS             2889..4430
                     /locus_tag="ISB30_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HAP1992032.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKNNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGTIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIRHPRLMESSLFM
                     PMEDA"
     gene            4427..>6493
                     /locus_tag="ISB30_004472"
     CDS             4427..>6493
                     /locus_tag="ISB30_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAP1992033.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRTYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMTLTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LKGDIYT"
BASE COUNT         1582 a         1605 c         1735 g         1571 t
ORIGIN      
        1 tgagaaatca agggcctttt ctttttgtcg aatatcgttc caggtatatt ccagtacttc
       61 gataaataaa ttttctttat taccaaagtg gtgataaacc gtctgtttac tgcaccccgc
      121 ggccttgacg atattatcga ctctggcccc ttcgtaacca tattcggcaa attcattaac
      181 cgcgcataac agcagctttt ctttcaggct ctgtacgttg ctggtatcaa gatacataac
      241 gcttccccac gacctcttcg tggtgactat cataacctga tttgatttca gcttcagaaa
      301 aaagggcaaa aacttgtact ttcatcaggt tatcaaatgt cctgtgatcg ccatcgcata
      361 tcaaccaact agatggttga tctttttatc ctccagtcgc aagctaatca tcactaagga
      421 aacaaaattc aaaaaaagag agctattgta tgaaatccac tcgtcctgtt gccgtgatta
      481 ccggagccgc tcgtggtatt ggaaagggct gtgcgctgga gcttgcgcgt ggcggattta
      541 atctgctgat taatgatcgt ccggatgcgg atagcgtaga aaaattacat gccacacaac
      601 aagaatgtct ggcggaaggc gtggaagtga tttgcttccc tgctgatgtg ggcgatctct
      661 ccctgcatga agagatgctc gacgcagcgc aaaaccagtg gggacgtctg gactgcctgc
      721 tcaacaacgc aggcatttca gtgaaaaaac gcggtgacct tctcgacctc gaaccagaca
      781 gcttcgacca aaatattgcc attaacaccc gcgcaccgtt cttccttgct caggcattca
      841 gtaagcgctt actggcacag ccgaaaccgg aagcagaact gccgcaccgc tcgattattt
      901 ttgtcagttc catcaacgcc atcatgctgg cgatgaatcg cggcgaatat accattgcca
      961 aaactgccgt ctctgccgct gctcgtctgt ttgctgctcg cctttgtaat gaacaaattg
     1021 gcgtatatga agtgcgtcct ggtctcatca aaaccgatat gactattccg gcaacggcct
     1081 attacgacga actgattgcc aaaggtctgg tgccgtgggg acgttggggt tatccggcgg
     1141 atattgcttc cactgtccgc gcgatggcgg aaggtaaact tatttacacc tgtggtcagg
     1201 cggtcgccat cgacggcggc ttaagtatgc cgcgcttctg aggctgaatc atgagagatc
     1261 tgttacaacg cccggattta ttcagtatca ataccgcgac actgggctat aaaacgccgc
     1321 tacccgccat tattgatgcc tgcgccgcac gcggtattgg cgcgattgcc ccctggcgca
     1381 gggagttgca gagtgaagat ctccagcaaa ccgcccgcca gcttgctgcc agtaacatga
     1441 acgtttccgg tttgtgccgt agcacctact acaccgcacc gacgctggcg gagcgcaaac
     1501 tggcgattga cgataaccgc cgggcgctgg acgacgccgc tgtactgaac gccgcctgtt
     1561 atatgcaggt ggttggcggt ctgccaatgg gaacaaaaga tctgtacgaa gcacgggaac
     1621 aggtgaaaca agggattcgc cagttgctgc cacattcgaa agaggtcggc gtgccaattg
     1681 cactggagcc actgcacccg atgactgctg ccaatcgttc ttgtctgtgc acgttacgtc
     1741 aggcgctgga ctggtgtgat gaactcgatc ccgatggcga atttggcctc ggcgtggcgg
     1801 tagatgtcta ccatgtctgg tgggacccgg atctcgccag tcagatcctg cgtgccggaa
     1861 aacgcatcct cgcgtttcat gtttccgact ggttagtgcc gaccaccgat ctggtcaatg
     1921 accgtggaat gccgggagat ggtgtcatca atattccgtc aattcgtcgg ctggttgaaa
     1981 acgccggatt taatggcgct attgaactgg aaatattctc accgtactgg tggcagaaag
     2041 atattaacag cacgctggat attagcgtcg atcgcatcgc gcattattgt taaggaatga
     2101 tcatgaatga ccaacccgtt ctgttcagca gagcggcggc ttcttgccgc ctgacattga
     2161 accgtgaaga taaatgtcat gctattaatg aagaaatgat cgaatctctc gaccattatt
     2221 taaatgaaat tgaaaatgac acggcattac gcttagtcga attaacggca accggcgata
     2281 aattcttttg tgcgggtggc gatattaaat cgtggtcggc ctattcgcca ttagatatgg
     2341 gcagaaaatg gattaaacgc ggtaatgacg tttttaatcg cctgcgaaac ttaccccaat
     2401 taaccgttgc gaatctcaac ggtcatacca tcggcggtgg tattgaactt gccttgtgct
     2461 gcgatattcg tatcgctcgc cccggcgcga aattctctaa cccggaagtg atgctcggca
     2521 tggtgccagg ctggatgggc attgagcgag tgcttaatca ggttggtcct gtggtcggtc
     2581 gccagatgct gatgctgggt aaacgcctga cggcgcagga agctcagaca gcgaatttaa
     2641 ttgatgaagt ggtagaaaaa gaacaggtgg aaagctggat ggcgaaccag ttagcgcagc
     2701 tggaaaaatg tggtccggtg gcactggcac atattaagca actgattctg gcgctggaaa
     2761 ataaacacgc cgattactcg caccagttgc tggctggatt aatgtccgcc actcaggatt
     2821 gccaacaggc gacgcacgca tttgccgaaa aagccggcgt gagcttccac aatcaataag
     2881 gaaattgtat gcgtaagtta tcaaccgccg agacactggc ggcgcaaatt caggacggcg
     2941 caaccattgc cattagcggc aacggcggcg gcatggtgga agctgaccat attctggccg
     3001 ccattgaagc gcgctttctg caaaccggac acccgcgcga tctgacatta atccactcgc
     3061 tgggtattgg cgatcgcgac agcaaaggca ctaaccgttt tgctcacgcc gaaatgctca
     3121 aacgcattat tgccggacat tttacctggt cgcccaagat gcaggaactg gtgaaaaaca
     3181 atgctattga agcctactgt ttccctggtg gcgtaattca ggcactgctg cgggagatcg
     3241 gtgccggacg tccggggctt tttacccacg ttgggctggg atcgtttgtt gatccacgca
     3301 atggtggcgg taagtcgaat gaatgcacta ccgacgacct ggtagaactg attgaaatcg
     3361 acggtgagac caaacttcgt tatcgccctt tcaaagtgga ttacgcgatt ttgcgtggca
     3421 cttatgccga tccacgaggc aacgttagcc tcgaagaaga agcgattgat atggatagct
     3481 attcgatggc gctggcagca cacaacagcg gcggcaaagt gttcgtacag gtacgcgatg
     3541 tgctggaagc aggtaccatt gaaccacgtc gggtcaagct accaggaatt ctggttgatg
     3601 gcatcgttga gcaccgcgaa caaccgcaaa cctatcttgg tggttacgac ctgaccatca
     3661 gcggtcaaca tcgccgtcta agttctaacg acgctattga actggttagt catccggtgc
     3721 gtcgcctgat tgcccgtcgg gcagcgcggg aactggtggc aggcgcttca accaactttg
     3781 gctttggtat tcccggcggt attcctgggg ttgcgctgcg cgaaggcgtg ccttatcaaa
     3841 gtttgtggct gagtgtagaa cagggtgtgc ataacggcat gatgcttgat gatgctctgt
     3901 tcggctgcgc ccgtaacgcc gatgccatta ttccatcact cgatcaattt gaattctaca
     3961 gtggcggcgg catcgatatc accttcctcg gcatgggaga gatggatcaa tacggtaacg
     4021 tcaacgtatc ccatctcaat ggcaatctga ttggccccgg cggatttctc gaaattgcac
     4081 aaaacgcccg taaagtggtg ttttgcggta cgttcgacgc caaaggcagc aagattgatg
     4141 taacgccaga tggcttgcat atcgcccagt caggtcaaat ccctaaactg gttacccagg
     4201 tggaaaaaat tacttttagc gccgcctacg cacagcaaag tggtcaggaa gtgttgtata
     4261 tcactgaacg cgcagtattc cagttaacgg cagaaggcgt tgaattaatt gaaatcgcgc
     4321 cgggtgtgga gattgagcgc gacattctgc cgtatatggc cttccgtccg attatcaggc
     4381 acccacgtct gatggaaagt agcctgttta tgccaatgga ggatgcatga gtcagcttga
     4441 tgacaccatc cttgatgcac tgacgcacgt tactttccca aagggttttg cacaggcaga
     4501 acccgcatgg gttgtcacgg ttgacggcgt tgattaccca ctctggaaaa cagatgccct
     4561 ggtagtcggc agtggtgcag ccgggctacg tgcagctgtg gaacttaaac gccgccagca
     4621 aaatgtgtta atcgccaccg ccgggttata tatggggacg tcggcatgtt ctggctctga
     4681 taaacaaaca ctgtttaccg ccgctactgc gggcaacggc gacaacttca ccaaactggc
     4741 agaggcactg gcgagcggtg gggcgatgga tcacgacacc gcttatgttg aagcggtagg
     4801 ttctctgcac actcttggcg ggttgcaata tcttggtctg gaattaccgg aagatcgcta
     4861 tggcgcgatt cttcgttatc aaaccgacca tgacgaagcc gggcgtgcaa cctcgtgtgg
     4921 gccgcgtacc tcaaggttaa tggtgaaagt gctgctggaa gaagtacagc gtcttgctat
     4981 cccaatgttg accagtgcaa cagtgattaa actgctgcat cagcgtgacg aaaacggtga
     5041 agaccgtgtg gcgggggcaa tcctcgcgac cggtcatcgc gcccataacc catgggggct
     5101 ggcaattgtg actgcgccca atgtggtact ggcaacagga gggcctggcg agctttatcg
     5161 cgacagtgtt tatccacaca aatgtttcgg ctcgctgggt ctggcgctgg aggaaggtct
     5221 gacgctaacc aatctgactg aaagccagtt tggtatcggc acaccgcgca gcacgtttcc
     5281 gtggaatttg tccggcacct atgtgcaggt gatcccgtat atctattccg tagatgctga
     5341 gggtaacgag tataacttcc tcgcggatta ctatcgcacc acccaggagc tagcttcaaa
     5401 catcttccgt aaaggctatc agtggccgtt ccacgccacg cgggtaatgg attttggctc
     5461 cagcttgtta gatatggcag tagcgcaaga gcagcaatca gggcgtcagg tatttatgga
     5521 tttcaatcgc aatcctgaag cggtgccggg tgacctgccc ttctcattag atcgactgga
     5581 cgacgacgtt cgcacgtatc tggaaaataa cgacgctctg gcaccatcgc ccatcgaacg
     5641 cctgcaacga atgaatccgc tgtctatctc gctgtataag atgcacggtt acgatctcac
     5701 cacgcagcca ttgcagtttg ccatgaataa tcagcacatg aatggcggca ttgaagtcga
     5761 tatctgggga caaacatctc tgcccggttg ttttgcggtg ggggaagttg ctggcacaca
     5821 cggcgtcacc cgacctggcg gcgcggcatt gaatgccggg caggtttttg ctgttcgtct
     5881 ggcacgtttt attggttgca cgcaaaagcg gaatattgat ggagatatag cacagctggc
     5941 agctccggca ctggcttcta taagagagat cattactcag gcgcacgata acgggaccgg
     6001 gatgccgttg tcggttgtga gagaaaaaat tcaggcacga atgtctgacc atgcgggatt
     6061 tatttgccat gccgataaag tccgacgcgc cactcgtgat gccctgctat tgaacgaatt
     6121 tgtccaacga catggattgg caatcaaaca tgtgggtgaa gttgccgagc tgtttatgtg
     6181 gcggcatatg acgctgactt ctgccgcggt attaacacaa ttaacgcatt atattgatgc
     6241 tggtggtggt agccgtgggg cgcgtatggt tattgatcca caaggcaaat gcctaccaca
     6301 aactcgtcgc ggcgcaaaag aagaatggcg ttttcgctct gaactcgctg aagacaaaaa
     6361 tcacaaatta acaattcaat attcgcaagg ttcttttatt accgaagtaa agtctttacg
     6421 tatgcaaccg tgtattaacg gtatttactt tgaaaaaaac tggccagact ttttaaaggg
     6481 agacatttac aca
//
LOCUS       DACADF010000215         6387 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.217, whole genome shotgun sequence.
ACCESSION   DACADF010000215 DACADF010000000
VERSION     DACADF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.217"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(325..1995)
                     /gene="betA"
                     /locus_tag="ISB30_004473"
     CDS             complement(325..1995)
                     /gene="betA"
                     /locus_tag="ISB30_004473"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HAP1992034.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVITSKPGVNPLFEA
                     MIEAGMQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFNIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPRIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPGILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKEALPRSTAGYFVANGMPVRAKK"
     gene            complement(2009..3481)
                     /gene="betB"
                     /locus_tag="ISB30_004474"
     CDS             complement(2009..3481)
                     /gene="betB"
                     /locus_tag="ISB30_004474"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HAP1992035.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPTKCKAAFEQKVLARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGNVLKGDSFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILIYESEDEVIRRANDTDYGLAAGIVTADLNLAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3495..4082)
                     /gene="betI"
                     /locus_tag="ISB30_004475"
     CDS             complement(3495..4082)
                     /gene="betI"
                     /locus_tag="ISB30_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HAP1992036.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAELRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4211..6244
                     /gene="betT"
                     /locus_tag="ISB30_004476"
     CDS             4211..6244
                     /gene="betT"
                     /locus_tag="ISB30_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HAP1992037.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1318 a         1741 c         1758 g         1570 t
ORIGIN      
        1 catgtggttt tggtctgact tatttttgta ctcgttacaa gaaccctttt cgtatatgct
       61 aaccgtgagc gggaatgaac aggtaaccat agccaggaac attagcggcg taattcttaa
      121 gctgagtggt atttcatctg tagtttatct gtgtatgcat agcacattca tcagccagat
      181 tgagcaagtc agcttagaat ttgctggcga gattgtcacg atgaagcatg agagttacct
      241 cagtgtttta tataaggatt cggtcctcat agtagaacgg taactctcaa tctgataggt
      301 tcctgagtca gttcacatca cgactcattt tttcgctctc accggcatcc catttgccac
      361 aaaatatccc gccgtgctcc tcggcaacgc ttccttccca cgaatcatat ccgctatttt
      421 ctcgccaatc ataatcgtcg tggcgttcaa attgccggtg ataatttgcg gcataattga
      481 cgcatccacc acgcgcaggc cttccagccc gtgtacgcgg ccttcgccgt caaccacagc
      541 catctcgtca taacccattt tgcaggtacc gcacggatga aacgcggttt cggcgtggtt
      601 acgcacgaac tcatcgagct gctcatccgt ctggcattcg acaccagggc tgatttcgcg
      661 gccacggtat tgatccagcg cgggttgatg catgatctcg cgtgtgatgc gaattgcgtc
      721 gcggaactcc tgccagtcct gctcgtgcga catgtagtta aacagaattc ccggatgctg
      781 gtgcgggtcg cgggatttaa tccgcacatg cccacggctt ggcgagcgca tcgatccgac
      841 atggcattgg aagccgtgct ctttcactgc gttcgagccg ttatagttaa tcgccactgg
      901 caggaaatgg tactgaatat tcggccacgc aaattcctca cggctgcgaa taaatccgcc
      961 tgcttcaaag tggttgctgg caccaacgcc ggtgccgcca aacagccact ccgcaccgat
     1021 tctcggctga ttccaccact gcagggcagg gtagagggaa accggttctt tgcactcgta
     1081 ttgcagatac atctccaggt gatcctgaag attttcgccg acgccgggta attcatgcac
     1141 cagcggaata ttaaactccg ccagcagttc agcgttgccg acgccggagc gttgcaggat
     1201 ctgcggtgaa gcaatcgcgc ctgcacataa cagcacttct ttattcgccg ccgcgcgggt
     1261 cggaatagtg ctgtcgcctt ccagccactc gacgcccacc gcgcgtttgc cgtcaaaaat
     1321 gatgtgatcg gtcatggcgt gagtacgaat ggtcaggtta gggcgcgatt tggcctgatc
     1381 gagatagcca cgcgcggtgc tggcgcgacg gccatgcggc gtgacggtgc gatccatcgg
     1441 gccaaaacct tcttgctgat agccgttgag atcgtccgtg cgtgggtagc ctgcctgcat
     1501 gcccgcttca atcatcgctt caaacagcgg attgacgccg ggtttggagg tgatgacgct
     1561 caccgggcca tcgccaccgt ggtaatcgtt ctcgcccaca tcgcgagtct cggccttgcg
     1621 gtagtagggc aggcagtcga gataactcca gttctccaga ccaggttctt gtgcccagtt
     1681 atcgagatcc agcgcattgc cacggatgta gcacataccg ttgatcagcg atgaaccgcc
     1741 cagacctttg ccgcgtccgc actccatgcg gcggttgttc ataaacggtt cgggttccgt
     1801 ctcataggcc cagttgtagc gtttgccctg tagcgggaac gccagcgcgg cgggcatctg
     1861 ggtgcggaag tcaaagcgat agtccgggcc gcctgcttca agcagcagca cggttgtgtt
     1921 cggatcttca gtcagacggg tagcgagaac gttgccggct gagccggcac caataatgat
     1981 gtagtcaaat tgcaaataaa cctcctggtt agaatatgga ctggaattta gccatctcaa
     2041 cctggatgga cttcacctgg gtgtaactct ggagcgtcat cacgccgttc tcgcgaccaa
     2101 tgccggagtg tttgtagccg ccaacgggca tctctgccgg ggattcgccc caggtgttga
     2161 tccagcaaat acccgcttcc agctgatgaa tgacgcgatg cgcgaggttc aggtccgccg
     2221 tcacgatacc cgccgccaga ccgtagtcgg tatcgttagc gcggcgaatg acttcgtctt
     2281 ccgactcgta gatcagaatg gacatcactg gcccgaaaat ctcttctcgc acaatggtca
     2341 tatcgtcgct gcaatcggtg aacacggtcg gtgcaaccca tgcgccgtta tcgaagctat
     2401 cccccttcag tacattgccg ccgcacagta cgcgcgcgcc ttcctctttg cctttggcga
     2461 tatagcgcag cacgttatcg cgatgcggga agctgaccag cgggccgaag ttagtttgcg
     2521 gatcgaaaac gtcgcccgcg cgaatgcgct caacgcgcgc cagaaccttt tgttcaaatg
     2581 cggctttgca tttcgtcgga acgaagacgc gagtgccgtt ggtacacacc tgaccggagc
     2641 tgaagaaatt cgccatcatg gcgatatcgg cggcgagatc gagatccgca tcgtcgaaaa
     2701 cgatcagcgg tgatttaccg cccagctcca tggtcacttc tttcagggag gaagctgccg
     2761 agttagccat cacttttttg ccgctggcga caccgccggt aaatgacact ttggcaatgc
     2821 ccggatgatc ggtcagatat tgtccggtct ccgcgcccac gcccggcaac acgttaaata
     2881 cgccgtccgg caggcccgct tcgctgtaaa tttcggccag ctttaacgcg gtaagcgggg
     2941 tgacttcgct cggtttgaaa atcattgcat tgcctgccgc cagcgctggg gcggatttcc
     3001 acaaagcaat ctggatcggg tagttccatg cgccaatccc cgccaccaca cccagcggtt
     3061 cgcggcgggt ataaacaaat gacgtctcac gcaacgggat ctggctgcct tccagcgccg
     3121 ggatcagccc ggcatagtac tccagcacgt ccgcaccggt aacgatatcg acggttgagg
     3181 tttccgaata tgcttttccg gtgtcaaggg tttccagttt tgcgagttcg tcattgcgtt
     3241 cacgcagaat atcaacggcc cgacgcagaa tacgcgagcg ctccatagcg gtcatcgccg
     3301 cccagatttt ttgcccttgt tgggcactct tcacggcgcg atcgacatcc tcacgcccgg
     3361 cggcctgcac ggtcgccagc acgttaccgt tggccgggtt aatggtctcg aaggtgcgac
     3421 cgctggtggc ggaggtataa ccaccatgta tataaagctg ctgttctgcc attcgggaca
     3481 tcacgtctcc tcggttaatc ggtgggtaaa tgctgagtaa taaagtgacg ggtcagcgag
     3541 tgtgcgagag gtttatccag cggtttgccg ctcagtgccg cgcgcagcca taatccgtca
     3601 attagcgcgg caaggccgta gcccgcttcc tgtgcctgtt ggcgcggtaa ctcgcggcga
     3661 aattcgctca ccagattcga caataagcgg cggctactga cctgctgcaa acggtaaagc
     3721 atcggctgat gcatactgct ggcccagaag gccagccagg ctttcatcgc cgcactgctc
     3781 acctgggttt catcgaagtt tccgccaaca atcgcctgta atcgcagctc tgcactgccc
     3841 tgcggaagtg catgtaatcg attcaaaacc gcgtcacgca gctgactggt gatatcgcgc
     3901 atggttgctt ccagcagacc atttttgtcc ctgaaatagt ggctgatgat ccccgtagaa
     3961 acgcctgcac ggcgggcgat ctgcgcgatc gttgcatcgt gcatgcccac ttcatttatt
     4021 gcttccagcg tggcgtcgat cagttgtctg cgccggatcg actgcattcc caatttgggc
     4081 attttcgcca ctccattcat cagcgttgtt tatctattaa agcggttatt gattggacgt
     4141 tcaatataaa atgtgtctta attgttacta atttgatttt aaatggtaac aataacagtg
     4201 gggatactgg atgacagacc tttcacacag cagggaaaag gacaaaatca atccggtggt
     4261 gttttacact tccgccggac tgattttgtt gttttccctg acaacgatcc tgtttcgcga
     4321 cttctcggcc ctgtggattg gccgcacgct ggactgggtt tctaaaacct tcggttggta
     4381 ctatctgctg gcggcaacgc tctatattgt ctttgtggtc tgtatcgctt gttcgcgttt
     4441 tggttcggtg aagctcgggc cagaacaatc aaaaccggaa ttcagtttgc tgagttgggc
     4501 ggcgatgctg tttgctgccg ggatcggtat cgacctgatg ttcttctccg tagccgaacc
     4561 ggtaacgcag tatatgcagc cgccggaagg cgcgggacag acgattgagg ccgcgcgtca
     4621 ggcgatggtc tggacgctgt ttcactacgg cttaaccggc tggtcgatgt atgcgctgat
     4681 gggcatggcg ctcggatact ttagctatcg ttataatttg ccgctcacca tccgctcggc
     4741 gctgtacccg atcttcggta aacggattaa cgggccgata ggtcactcag tggatattgc
     4801 tgcggtgatc ggcaccatct tcggtattgc cactacgctc ggtatcggtg tggtgcagct
     4861 taactatggc ttgagcgtgc tgtttgatat tcccgattcg atggcggcga aagcggcact
     4921 gatcgccttg tcggtgatca tcgccactat ctcggtgaca tccggtgtcg ataagggcat
     4981 tcgcgtgtta tcggaactca acgttgcgct ggcgctggga ttgattctgt tcgtgttgtt
     5041 tatgggcgac acctcgttcc tgcttaatgc actggtgctg aacgttggcg actatgtgaa
     5101 tcgctttatg ggcatgacgc tcaatagttt tgccttcgac cgtccggttg agtggatgaa
     5161 taactggacg ctcttcttct gggcatggtg ggtggcatgg tcgccgtttg tcggcttgtt
     5221 cctggcgcgt atctcgcgtg ggcgtaccat tcgccagttc gtgctgggca cgttgattat
     5281 tccgtttacc ttcacgctgt tatggctctc ggtattcggc aatagcgcgc tgtatgaaat
     5341 catccacggc ggcgcggcat ttgccgagga agcgatggtc catccggagc gcggcttcta
     5401 cagcctgctg gcgcagtatc cggcgtttac ctttagcgcc tccgtcgcca ccattactgg
     5461 cctgctattt tatgtgacct cggcggactc cggtgcgctg gtgctgggga atttcacctc
     5521 gcagcttaaa gatatcaaca gcgacgcacc cggctggctg cgcgtcttct ggtcggtggc
     5581 gattggcctg ctgacgctag gcatgctgat gaccaacggg atatccgcgc tgcaaaacac
     5641 cacggtgatt atgggcctgc cgttcagctt tgtgatcttc ttcgtgatgg cggggttgta
     5701 taaatctctg aaggtagaag attaccgccg tgaaagtgcc aaccgcgata ccgcaccgcg
     5761 accgctgggg cttcaggatc gcctgagctg gaaaaaacgt ctctcgcgcc tgatgaatta
     5821 tccgggcacg cgttacacta aacagatgat ggagacggtc tgttatccgg caatggaaga
     5881 agtggcgcag gaactgcggt tgcgcggcgc gtacgtggag ctaaaaaacc tgccgccgga
     5941 agagggacaa cagttggggc atctggattt gttggtgcat atgggcgaag agcaaaactt
     6001 tgtctatcag atttggccac agcaatattc ggtgccgggc tttacctacc gcgcacgtag
     6061 cgggaaatcg acctactacc ggctggaaac cttcctgtta gaaggcagcc agggcaacga
     6121 cctgatggac tacagcaaag agcaggtgat caccgatatt ctcgaccagt acgagcggca
     6181 ccttaacttt atccatctcc atcgtgaagc gccgggccat agcgtgatgt tcccggacgc
     6241 gtgattgtta ttgcataaaa ccgcgccatg tctgcatatg gcgcggtttc tcttacctct
     6301 tgatacgtta tatctgtgta cagttaagcc cttagtatcg attgatgatt gccaggcaac
     6361 agataataaa aaagaaaaga actattg
//
LOCUS       DACADF010000216         6239 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.218, whole genome shotgun sequence.
ACCESSION   DACADF010000216 DACADF010000000
VERSION     DACADF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.218"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..212)
                     /locus_tag="ISB30_004477"
     CDS             complement(<1..212)
                     /locus_tag="ISB30_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992038.1"
                     /translation="MLNTRVHQSEVSMATDTPRIPEQGVATLPDEAWERARRRAEIIS
                     PLAQSETVGHEAADMAAQALGLSRRQV"
     gene            complement(251..919)
                     /locus_tag="ISB30_004478"
     CDS             complement(251..919)
                     /locus_tag="ISB30_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAP1992039.1"
                     /translation="MAQAAARGQLDLHYQPLVDLRDHRIAGAEALMRWRHPRLGLLPP
                     GQFLPLAESFGLMPEIGAWVLGEACRQMHKWQGPAWQPFRLAINVSASQVGPTFDDEV
                     KRVLADMALPAELLEIELTESVAFGNPALFASFDALRAIGVRFAADDFGTGYSCLQHL
                     KCCPITTLKIDQSFVARLPDDARDQTIVRAVIQLAHGLGMDVIFRRRLHQLIGRNGCC
                     AASS"
     gene            complement(955..1191)
                     /gene="merE"
                     /locus_tag="ISB30_004479"
     CDS             complement(955..1191)
                     /gene="merE"
                     /locus_tag="ISB30_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HAP1992040.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            complement(1188..1550)
                     /gene="merD"
                     /locus_tag="ISB30_004480"
     CDS             complement(1188..1550)
                     /gene="merD"
                     /locus_tag="ISB30_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HAP1992041.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            complement(1568..3262)
                     /locus_tag="ISB30_004481"
     CDS             complement(1568..3262)
                     /locus_tag="ISB30_004481"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HAP1992042.1"
                     /translation="MSTLKITGMTCDSCAVHVKDALEKVPGVQSADVSYAKGSAKLAI
                     EVGTSPDALTAAVAGLGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAV
                     IGSGGAAMAAALKAVEQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
                     FDGGIAATTPTIQRTALLAQQQARVDELRHAKYEGILEGNPAITVLHGSARFKDNRNL
                     IVQLNDGGERVVAFDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIG
                     SSVVALELAQAFARLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQ
                     VAYINGEGDGEFVLTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDP
                     GMRTSVEHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQV
                     ATVGYSEAEAHHDGIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVA
                     PEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            complement(3314..3736)
                     /gene="merC"
                     /locus_tag="ISB30_004482"
     CDS             complement(3314..3736)
                     /gene="merC"
                     /locus_tag="ISB30_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HAP1992043.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
     gene            complement(3772..4047)
                     /gene="merP"
                     /locus_tag="ISB30_004483"
     CDS             complement(3772..4047)
                     /gene="merP"
                     /locus_tag="ISB30_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HAP1992044.1"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKAL
                     SKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            complement(4061..4411)
                     /locus_tag="ISB30_004484"
     CDS             complement(4061..4411)
                     /locus_tag="ISB30_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HAP1992045.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            4483..4917
                     /gene="merR"
                     /locus_tag="ISB30_004485"
     CDS             4483..4917
                     /gene="merR"
                     /locus_tag="ISB30_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HAP1992046.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLREPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLKDVREKMAD
                     LARMETVLSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
     gene            4996..6000
                     /locus_tag="ISB30_004486"
     CDS             4996..6000
                     /locus_tag="ISB30_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HAP1992047.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
BASE COUNT         1259 a         1875 c         1912 g         1193 t
ORIGIN      
        1 acctggcgcc gagacaagcc cagcgcctga gccgccatat cggccgcttc gtgcccgacc
       61 gtctccgact gcgccaacgg actgatgatc tccgcacgac ggcgcgcacg ctcccaagcc
      121 tcatcaggca gagtggccac gccttgttct ggaatccgtg gggtgtccgt cgccatgctc
      181 acctcgcttt ggtgcacacg agtattgagc atagtcgaga ttggtgcaga tcacttctga
      241 tattgaactg tcaggagctg gctgcacaac agccattacg cccaatcaac tggtgcagtc
      301 gtcttctgaa aatgacatcc atgcccagcc cgtgcgcgag ctggatcacc gcccgcacga
      361 tagtttggtc acgggcatca tccgggagcc tggcgacaaa ggattggtcg attttcaatg
      421 tggtgatggg gcagcatttc agatgttgca ggcaggaata gccggtgccg aagtcgtcgg
      481 cggcgaagcg cacgccgatg gcgcgcaagg cgtcgaaact ggcgaacagg gctggattgc
      541 cgaatgcgac cgattcggtc agttcgatct ccagaagctc ggcgggcagg gccatatcgg
      601 ccagcacccg ctttacctcg tcgtcgaacg ttggcccaac ctggctggcg gacacattga
      661 tggcaagacg gaacggttgc catgccggtc cttgccactt gtgcatctgg cgacaggcct
      721 cgcccagcac ccacgcgcct atttccggca tcaggccgaa cgactcggcc agcggcagga
      781 actggccggg cggcaacagg ccaagcctcg gatgccgcca gcgcatcaac gcttccgcgc
      841 cagcgatccg gtgatcgcgc agatcgacca gcggctggta atgcaggtca agctgtccgc
      901 gcgccgccgc ctgcgccaac tcggccgccg tccatccggc gggctgcgaa ctcgtcatga
      961 tccgccccgg aaggcgcgca gcagccgcgt tacggccaga acgaacaagc cggtcagcgc
     1021 gagcgcggca acaccccaat gctcgccaag gaaggcaccg gcggtcgtcc cggccagcac
     1081 ggcggcgaga atcggcagat ggcaggggca ggtcaacacg gccagcgcac cccacaggta
     1141 gccggaaacg ggttggcgcg tctcgggcgg cagtttgtca ggggcgttca cggcaatgcc
     1201 tcctcgtgcg cccgctcggc tggcatggag gccagttgcg cgtccagatg ggccaacgcc
     1261 gcgcgccgcc gctcgaccaa ctggcgcagc acggcaagct gcgctgcggc ttgtgcgccg
     1321 tccgctgcgt cgagcgcacg gcacagccgc gccagggcat ccaggccgat acccgcctcg
     1381 aaggccgcgc gcacgaagca cagccgttgc aaggccgcat cgtcgaacac gccgtagccg
     1441 cccgtggtgc aggccaccgg ccgtaacaag ccgcgcacca ggtagtcgcg cacgatatgt
     1501 acgctcaccc cagcgttatg ggccagttgc gataccgtgt aggcgctcat cgcacacctc
     1561 cttgtcctca cccggcgcag caggaaagct gcttcacatc cttgttgaag gtctgcgccg
     1621 cgagcttcaa cccttcgacc atcgtcaggt aggggaacaa ctggtcggcc agttcctgca
     1681 ccgtcatccg gttgcgaatc gccagtgcgg ccgtctggat cagttcgccc gcttccgggg
     1741 ccactgcctg cacgccgatc agtcgtccgc tgccttcttc aaccaccagt ttgatgaagc
     1801 cgcgcgtgtc gaagttggcg agcgcgcgcg gcacgttgtc cagcgttagc gtgcgactat
     1861 cagttttgat gccgtcatgg tgcgcttccg cctcgctgta gcctacggtc gccacttgcg
     1921 ggtcggtgaa caccacggcc ggcatcgcgg tcaggttcag ggccgcgtca ccgccggtca
     1981 tgttgatcgc ggcgcgagtg ccggccgctg ccgccacata gacgaactgc ggctggtcgg
     2041 tgcagtcgcc tgcggcgtag atgtgttcca cgcttgtacg catgccgggg tcgatgacga
     2101 tagcgccttg cggggtgagc gtgacgcccg tcgcatccag tgccagcttg cgtgtgttgg
     2161 gcgcgcggcc ggtggcgacc agcagcttgt cggcgcgcag ttcgccgtgc gccgtggtga
     2221 gcacgaattc gccgtcccct tcaccattga tatacgcgac ctggctggcc tgggtgtgtt
     2281 ccctcacctc gatgccctcc atgcggaatg cggccgtgac ggcttcgcct atagctgggt
     2341 cttcgcggaa gaacagcgtg ctgcgagcca ggatcgtcac cttcgctccg agtcgggcga
     2401 acgcctgcgc cagctccagc gccaccactg atgagccaat cacggccagg cgcttaggaa
     2461 tcgtctcgct gaccagcgct tcagtggaag tccagtacgg agtgtctttc aggccgggaa
     2521 tcggcggcac ggccgggctc gcgccggtgg cgatcaggca gcggtcgaat gccaccacgc
     2581 gctcgccgcc gtcgttgagt tgcacgatca ggttgcgatt gtccttaaag cgggcggagc
     2641 cgtgcagcac agtgatcgcc ggattgccct ccaagatgcc ttcgtacttg gcgtggcgca
     2701 gttcatcgac gcgggcctgc tgctgggcca gcagcgccgt gcgctggatg gtcggcgtgg
     2761 tagcggcgat gccgccatcg aacgggcttt cccggcgcag atgggcgata tgggcggcgc
     2821 ggatcatgat cttggacggc acacaaccga cattgacgca ggtgccgccg atggtgccgc
     2881 gctcgatcag cgtgacacgt gcgccttgct cgacggcctt cagcgccgct gccatcgcgg
     2941 ccccgccgct gccgatgacg gcgatatgca atgcgccgct gctacccgtc ttgtcgtttc
     3001 tgcccagcag atcgcgcatc ttgtcgagca atccgcccgg cgtcgaaact gagggggcat
     3061 cggccagcgt ggcccgataa ccgagtccag ctacagcggc cgtcagcgcg tcgggtgacg
     3121 tgccgacctc aatggcgagc ttggcgctgc ccttggcgta ggagacatcc gctgattgca
     3181 cgccgggcac tttctccagg gcgtccttga catgcactgc gcacgagtcg caagtcatgc
     3241 cggtgatttt gagagtgctc ataccatcgt tccttattcg tgtgggccgc cgtgtcgcac
     3301 ggtcagccgt ctttcacaag cgcttggcgg ggagttcgca gccgtccggt ccgcaacggc
     3361 gatgcgccgg cgacacgaag tcccagatcg acaccccaat catcaaggcc aggccgacgt
     3421 acatcaggtt cgccgtccac cagttgccga gcagccagac cgtggccgca aacacgatgg
     3481 ccgggccgat catgccgagc agactgcgca gccattgccg atgactgaac caacccagcg
     3541 cgttcgccag gaaggccagc gcggcaaaca gcggcagcag gcggctgatg aacagtccct
     3601 cgtactggct caagaagccc agcccgatgg ccgcgccgaa gctggcgagg gctggaaagc
     3661 aggcggcgca gcccatcgcg gaaacgacgc tgccgagcgc gccggtttta tcggcaatgc
     3721 gtgtcatcag tcccatgaag cggctctcgc tgttgtcgtt ggcttgctgg ctcactgctt
     3781 gacgctggac ggatagccgg cgtctgcggt ggccttggtc agcttctgta cgctggcctt
     3841 ggtgtcgtca aaagtgacga cggcctcgcg cttctcgaag cccacatcga ccttgctcac
     3901 gccttcgacc ttggagagcg ctttcttgac tgtgatcggg caggcggcgc aagtcatgcc
     3961 gggaaccgct agcgtgacgg tctgggtagc ggcccacacc ggggcaacag cggcggcgag
     4021 ggcaagggag gcaaacagtt tcttcatgat gaactcctgg ttaatagaaa aatggaacga
     4081 catagggaaa tccaagcgcg accaggacca gcacggccac gatccagaaa atcagcttgt
     4141 aggtggcgcg cacctgcgga atcgcgcaga cctcacctgg cttgcatgcc tgcacgggcc
     4201 ggtaaatccg cttccaggcg aagaacagcg ccactagcgc cgcgccgatg aacaacggtc
     4261 gatagggttc cagcaccgtc aggttgccga tccaagcacc ggagaagccc agggcgacca
     4321 gtactagcgg ccccaggcag caggtcgatg caagaatggc ggccagcccg ccggcgaaga
     4381 gcgcaccgcg cccgttttgt ggttcagaca tacgttggcc cttttgaatt tggattggat
     4441 agcgtaacct tacttccgta ctcatgtacg gagtcaagcg atatggaaaa taatttggaa
     4501 aacctgacca ttggcgtttt tgccaaggcg gccggggtca acgtggagac aatccgcttc
     4561 tatcagcgca agggcctgtt gcgggaaccg gacaagcctt acggcagcat ccgccgctat
     4621 ggggaggcgg acgtggttcg ggtgaaattc gtgaaatcgg cacagcggct ggggttcagt
     4681 ctggacgaga ttgccgagct gttgcggctc gacgatggca cccactgcga ggaggccagc
     4741 agcctggccg aacacaagct caaggacgtg cgcgagaaga tggccgactt ggcgcgcatg
     4801 gaaaccgtgc tgtctgaact cgtgtgcgcc tgccatgcac gaaaggggaa tgtttcctgc
     4861 ccgttgatcg cgtcactaca gggcgaagca ggcctggcaa ggtcagctat gccttagcgt
     4921 gctttattta atgagatggt cactccctcc ttcccagtac tatgctgagg acaggctttc
     4981 attcggagaa ccatcatgga aaacattgcg cttattggta tcgatctggg taagaactct
     5041 ttccatattc attgtcagga tcatcgtggg aaggccgttt accgtaaaaa attcacccga
     5101 ccaaagctaa tcgaatttct ggcgacatgc ccggcaacaa ccatcgcgat ggaagcctgt
     5161 ggcggttctc actttatggc acgcaagctg gaagagttag ggcattttcc aaagctgata
     5221 tcaccgcaat ttgtccgccc attcgttaaa agcaacaaaa atgacttcgt tgatgctgaa
     5281 gctatctgtg aagcagcatc acgtccatct atgcgtttcg tgcagcccag aaccgaatct
     5341 cagcaggcaa tgcgagctct gcatcgtgtc cgtgaatccc tggttcagga taaggtgaaa
     5401 acaactaatc agatgcatgc ttttctgctg gaatttggta tcagcgttcc gcgaggtgct
     5461 gccgttatta gtcgactgag tacccttctt gaggacagta gtttgcctct ttatctcagc
     5521 cagttactgc tgaaattaca acagcattat cactatcttg ttgagcagat taaagatctg
     5581 gaatctcagt tgaaacgaaa gttggacgaa gatgaggttg gacagcgctt gctgagtatt
     5641 ccctgcgttg gaacgctgac tgccagtact atttcaactg agattggcga cgggaagcag
     5701 tacgccagca gccgtgactt tgcggcggca acagggctgg taccccgaca gtacagcacg
     5761 ggaggtcgga cgacattgtt agggattagc aagcggggca acaaaaagat ccgaactttg
     5821 ttggttcagt gtgccagggt attcatacaa aaactggaac accagtctgg caagttggcc
     5881 gactgggtca gggagttgtt gtgtcggaaa agcaactttg tcgtcacctg tgctctggca
     5941 aacaagctgg ccagaatagc ctgggcactg acggcgcgac agcaaactta cgaagcataa
     6001 aggcagaaat acaccagttt aaacaatcat tcatctggtt ttgcgaatac tgatattgat
     6061 gatactaacg gcccaccggc ctgttgagga acctgtaaaa cggaaaggct cattgaagcc
     6121 gtatattttc tggaggttca tcaggcgcgg aactcatcga ggcgcgggaa taaaatccca
     6181 ttcagacgcc ggatagattc aagcaagcca acttgtcgtc aaaatcggtg ttgcaaaaa
//
LOCUS       DACADF010000217         6191 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.219, whole genome shotgun sequence.
ACCESSION   DACADF010000217 DACADF010000000
VERSION     DACADF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.219"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(600..872)
                     /locus_tag="ISB30_004487"
     CDS             complement(600..872)
                     /locus_tag="ISB30_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HAP1992048.1"
                     /translation="MKMMRMYCPTCQAVARIGKTNRKHPQLYDVYCYCSNVECGHSFV
                     MNVAFSHSVSPSALNGQGRVKELIDAIPPEEREKALKLLLAAQKNG"
     gene            complement(1155..3842)
                     /locus_tag="ISB30_004488"
     CDS             complement(1155..3842)
                     /locus_tag="ISB30_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP1992049.1"
                     /translation="MKTPSVSTISGAAIGRWPYILSALGIKVPSAGHHGACPACGGKD
                     RFRLDDKAGRGTWFCNQCGHGDGLDLVRLVTGRKIKEVAGMVSEALALPEIQEKPALP
                     ARKKAAGKEAGAERYTRLRQQSCNGEPVYLTNKSLHGYSLPLLSQPLNLAGITFSTGS
                     LLLPLTDISGNITGGQLINPDGDKSLLPGSQLSGAFIALTDIPAETPEQVIITEGFAT
                     ALTVSLLTEGWIVAAVAATNLLKVTEQIRKRWPETRIILAGDNDLADGKENTGRIQAE
                     KAAKAVDGWVTLPPVRHKADWDDYRQEVGKERARDAFREEMTLHGKGQTRLPEGFRLT
                     KEYLWYDKLVNKSDGDTEIRNIKICSPLRVTAITSDADGSNYGRLLEWEDTNGNSRKW
                     AMPMEMLGGSGEELRRVLLVNGLSYININGMARAFLMEYISLCKPDRKVTCVNKTGWH
                     GGVYVLQDEVIGREAQSVILQTSSVQGRDFRVSGTSEGWRENIGRYCIKNARLAFAVS
                     LAFAAPLLKLVGIGGGGYHLKGESTDGKTTTMKVAASVCGGTDFWHTWRATGNALEGT
                     ASRRNDATLMLDEIREVDGREAGNIAYMLANGQGKARARTDGSVRETNRWNLLFLSTG
                     ELSLVEHAASAGERTYAGVEVRMIQIPSDSGKYGVFEELHGFSSGKTLAEHLEQHVAH
                     YHGAPFRDWLYCLTADLPELTSQAKALLKEYTRRLTPENAGNQVGRAVTRFALVAMAG
                     ELATKAGITGWPEGEAFRAAQSCLAAWMADRGHTANQEDKAALEQVRDFMTRNQFSRF
                     ADWNDDRNRPVSMMGFRKVDKGDNVTEPVVTFYVLPSGWKEICKGFDSRKVARLCVDA
                     GWLKPGEDGRTQNSIRLPEIGLKRVYQFNTQVLGSAEPE"
     gene            complement(3839..4249)
                     /locus_tag="ISB30_004489"
     CDS             complement(3839..4249)
                     /locus_tag="ISB30_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992050.1"
                     /translation="MNNNQNLKAHALRTAVLRYYIADAFISLMVRVHNEPVYIEDGER
                     IELSHEKVVSNIIYHIEMPWVNEFGADAGCILAAEKLEKMLKPGFMSENIRLSVFGVT
                     EMREVYRDIIFGAPDGELPEGFEFVKPEGQEVLL"
     gene            complement(4242..4478)
                     /locus_tag="ISB30_004490"
     CDS             complement(4242..4478)
                     /locus_tag="ISB30_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992051.1"
                     /translation="MKSMNFKQGCILSEQGESRLLRAALASEFLAEVLSVPTVNGART
                     VSAEGAAALVACIAEQLDGVVKETSTIKGVMRDE"
     gene            complement(4475..5065)
                     /locus_tag="ISB30_004491"
     CDS             complement(4475..5065)
                     /locus_tag="ISB30_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAP1992052.1"
                     /translation="MPALINPGNDQFSVINFLPHQECAGYSLKVAAKSAAGRRNPCYL
                     KAILHALGVFFCVAALTHLKFMVWWLIVFFYFLRHIIRIMVVQAGQLSGWPVSFRAGI
                     PTPVWATTHERRNSGGSVTRYLKEVAIMATVPALSHPEFTFVFLAVRRTDRDARPRPV
                     RVIAECEHAARLKLATEFILSFAARIPVKRAGEVVA"
     gene            complement(5153..6157)
                     /locus_tag="ISB30_004492"
     CDS             complement(5153..6157)
                     /locus_tag="ISB30_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HAP1992053.1"
                     /translation="MYAFKTERPDAARRYHNKFVTGTNYQEESNRRFALSEPTENILR
                     TSILESGWLIKNITLRDVNAVSGNAVNIGASELYTGRVDGGRFHKKMAINGTEFFLTE
                     TDTCARISYSDMSHIYHTGVPDEFEKTVESFFAQAYALDMLRVGFNGVSIANTTNPEI
                     NKKGEDVNIGWHALAKAYGNGKQIISEPVTLGETGTWKNIDALANHLITELIAEQFRE
                     DPRLVVLVGAELAAHQRLKLFNAADRPSDVNAAQMATSSVAGRFAFIPPFMPGKRLAV
                     TTLDNLHIYTQAITRWFRAEFDDESSEYVHSYLRNEGYALGEPELYAAVDECTLTFAD
                     "
BASE COUNT         1541 a         1710 c         1337 g         1603 t
ORIGIN      
        1 tcaaaaaaaa cgagtacgca agatacgaaa gtacttttct tgttcaccag ttagcttacc
       61 ttgatcctca ccagcgactg gaagataggt agcagtatat gtggaaacct tcccctagct
      121 aggtctacaa acgtagtttg ggcatttagg ctccacgatt cacggtttga cacggcctga
      181 tatctgcaca ttcgttttga tatgcagcaa tcgtgaaaaa ggaaaatata cgctcgacag
      241 accaaaacgg cactacaccg cacccgcctg cggttttcat atcactaaaa attttcagcc
      301 tggatttttt acaaacgata ccgccagtcc gtgccagtcc tcgagatgta ctgcgataca
      361 ggaactgaaa agaatgaaaa gaaattcatt ttttttcagt tttactgtcc gtcgaatcta
      421 tggcctgaat acgcaactct atgatattaa atggataatt aattttaggt gtgtgatttt
      481 atgaaggttt gtgaaacatg aagaagataa ccagttgaaa aataataatt ttatgaccgt
      541 ataaactgaa atcctgtgtt caggttccgg gaacagatcg gccattcccg acgcatactt
      601 tacccattct tttgcgcagc cagcagcaac tttaaagcct tttcccgttc ctcgggtgga
      661 attgcatcaa tcagctcttt aacccttccc tgcccgttca gcgcgcttgg actaacggaa
      721 tgggagaaag caacattcat cacaaacgag tgaccgcact ccacattaga gcaatagcag
      781 tacacatcgt acagttgtgg gtgtttcctg tttgttttac ctatccgtgc tactgcctga
      841 catgtggggc agtacattct catcatcttc atcgttctgt gccctatact gttgcccgcc
      901 agaagaaggg atttggatcc ctacctcttg ttctgaacgt gtaacactgg taacacgtgt
      961 aacgctattg atttaaaagg atttaatctg ttaccagtta gcaaaatcaa gaggtaacgc
     1021 gtggtaacac accatttttt gttacctctg ttaccagtca gtatttttga ctggtaacac
     1081 tgaagaaccg catcagtaac gggtgttacc tctgttacca gtgttacctc ataaaaataa
     1141 gactcacgcg ataatcactc aggttcagcg cttcccagta cctgtgtatt gaactggtac
     1201 acacgtttaa gccctatttc tggcaggcga atgctgtttt gcgtccggcc atcttcaccg
     1261 ggtttcagcc agcccgcgtc cacacacaac ctggccacct tacgcgagtc aaatccctta
     1321 cagatctcct tccagcctga cgggagaacg tagaacgtca caaccggctc tgtcacgttg
     1381 tctcctttat cgacttttct gaatcccatc attgaaacag gccggttcct gtcgtcattc
     1441 cagtcagcaa accgactgaa ctgattacgc gtcatgaagt cccgaacctg ttcaagtgcc
     1501 gctttatctt cctgattagc tgtatggccc cggtcagcca tccatgcagc cagacagctt
     1561 tgagcggcac gaaaggcctc tccctccggc catcctgtga tgcccgcttt tgtggccagc
     1621 tccccggcca tcgcaaccag agcaaaacgg gtaacagccc ggccaacctg attcccggca
     1681 ttttccggcg tcagccttcg ggtatactct ttcaataagg ctttcgcctg actggtcagt
     1741 tccggcagat cagcggtcag gcaatacagc cagtcgcgga atggtgcacc atgataatgc
     1801 gctacgtgct gctcaagatg ctcagccagg gttttcccgc tactgaagcc atgaagttct
     1861 tcaaacacgc catacttgcc cgaatcgctt gggatctgga tcattctgac ctcaaccccg
     1921 gcatacgtac gttccccggc gcttgccgca tgctctacca gtgacagttc cccggtagac
     1981 agaaacaaca gattccagcg gttggtttcc cttaccgaac catctgttct ggctctggct
     2041 ttgccctgcc cgttagccag catataagcg atatttcccg cctccctgcc gtcaacctcc
     2101 cggatttcat ccagcatcag cgtggcatca ttgcggcggc tcgccgttcc ttctaaggca
     2161 ttccccgtcg cccgccacgt atgccagaaa tctgttccac cacatactga tgcagccact
     2221 ttcatcgtcg tggttttacc gtctgtggat tcccctttga ggtgataacc tccaccgccg
     2281 ataccaacca gtttcagaag gggggcagca aatgccagac tcaccgcaaa ggcaagacgg
     2341 gcattcttta tgcaataacg gcctatattt tcacgccagc cctctgatgt gccgctgaca
     2401 cgaaaatcac gcccctgcac actcgatgtt tgtaagatga ctgactgggc ttcgcgccct
     2461 atcacttcat cctggagaac gtaaaccccg ccgtgccagc cagttttatt cacacaggtt
     2521 acttttctgt ccggtttaca cagcgagata tattccatca ggaatgctct ggccatgccg
     2581 ttaatgttga tgtaagaaag cccgttaacc agcagaacgc ggcgcagttc ttccccgcta
     2641 ccgcccagca tctccatcgg cattgcccat ttacggctgt ttccgttggt atcttcccat
     2701 tccagcaaac gcccgtaatt acttccatca gcatcactgg tgattgccgt cacccgcaac
     2761 ggactgcaaa ttttgatatt acgtatctct gtatcaccat cagacttatt aaccagcttg
     2821 tcgtaccaca aatattcttt tgtcagccgg aacccttctg gtaaacgggt ctgccctttg
     2881 ccatgcagcg tcatttcttc acgaaaggca tctctcgccc gttctttccc cacttcctgc
     2941 cgataatcat cccagtcagc cttatggcgt accggaggga gcgttaccca gccatccacc
     3001 gctttggcgg ctttctctgc ctgaatacgc ccggtgtttt ctttgccgtc agccagatca
     3061 ttatctccgg ccagaatgat ccgggtttcc ggccagcgtt ttcggatctg ctccgtcacc
     3121 ttcagtaaat tggtggcagc gacagccgcc acgatccatc cttcagtcag cagactgacg
     3181 gtaagcgccg tggcaaaacc ctcagtgata atcacctgtt caggtgtttc agcgggtata
     3241 tcggtcaggg caatgaatgc gccagacagc tggctaccgg gcagcagact tttatccccg
     3301 tcaggattga taagctggcc accagtaata ttcccggaaa tatccgtcaa aggcagcagt
     3361 aatgaaccgg tggaaaatgt tattccggcc agattcaaag gctgtgacag caagggaagt
     3421 gaatacccgt gtaagctctt atttgtcaga tagaccggct caccattgca ggactgctgt
     3481 ctgagtctgg tatatcgctc cgcgcccgct tcttttcctg cggccttttt cctggctggc
     3541 aatgcgggct tctcctgtat ttctggtaat gcaagcgcct cagataccat cccggcgact
     3601 tcttttatct ttcttcctgt caccagacga accagatcaa gaccatcgcc atgaccgcac
     3661 tgattacaaa accacgtccc tcgccccgct ttatcgtcca gtcgaaaacg atcttttcca
     3721 ccacaggcag ggcaagcccc atgatgtcct gcggacggta ctttaatacc taatgctgac
     3781 aggatataag gccagcgccc aattgcagct ccggaaatcg tggatacaga cggtgttttc
     3841 acagcagcac ctcctgacct tccggcttca caaattcaaa accttcgggt agttcgccat
     3901 caggtgctcc gaaaataata tcccggtaaa cctcacgcat ctctgtgaca ccaaaaactg
     3961 acaggcgaat attttctgac ataaagcctg gtttaagcat tttttccagt ttctctgcgg
     4021 ccagtatgca accagcatca gccccaaact cattcaccca cggcatttca atatggtaaa
     4081 taatattact caccactttt tcgtgggaga gttctattcg ttcgccatct tcaatataaa
     4141 caggttcatt atgaacacgc accattaagg aaatgaatgc atcagcaatg tagtagcgta
     4201 agactgctgt tcgtaaagca tgcgccttta aattttgatt attattcatc gcgcatcact
     4261 cccttaattg tgctggtttc cttaacaacg ccatccagtt gttcggcaat acaggccact
     4321 aaagccgcag cgccttccgc tgaaacagtt ctcgctccat tcactgtggg cacggacaag
     4381 acctctgcaa gaaactcact ggccagtgcc gcccgtaaca gacgactctc cccctgttca
     4441 ctcaatatgc agccttgctt aaaattcatg gatttcatgc aaccacctcc ccggctcttt
     4501 tcacagggat acgggcagca aagctcagga taaattcagt ggcaagttta aggcgtgcag
     4561 catgctcgca ttctgcgatt acacgcacag ggcgtggacg ggcatcacgg tctgtacggc
     4621 ggacagccag aaagacaaag gtaaattcag ggtgagataa agcagggact gtagccataa
     4681 tggcaacctc cttcagatag cgggtaacgc taccaccgga gttcctacgc tcatgggtgg
     4741 tagcccagac gggggtagga ataccggctc tgaaggatac cggccagccc gaaagctgcc
     4801 ccgcctggac taccataatt ctgatgatgt ggcgtaaaaa ataaaaaaat acaatcagcc
     4861 accacaccat aaattttagg tgagtcaaag ctgcgacaca aaaaaacacg cctagcgcgt
     4921 gtagtatcgc cttcagataa cacgggttcc tacgcccggc tgccgatttt gcggcaacct
     4981 ttaaactata tcccgcgcat tcctggtgag gcaagaaatt tataaccgaa aactgatcat
     5041 tgcctggatt aattaacgct ggcatgctta agcctctgct taaaaatagt tcttcatttt
     5101 tcctgatgct gtaaacgaga cccgcctctg agcgggtctt tctgtcgcta agtcaatccg
     5161 caaacgtcag ggtgcactca tcaacagccg cgtacagttc aggttcaccg agtgcataac
     5221 cttcattgcg caggtaggaa tgcacgtatt cactgctttc atcatcaaac tcagcacgaa
     5281 accagcgtgt tatcgcctga gtgtaaatat gcagattatc cagcgtcgtt actgccagac
     5341 gcttacccgg cataaacggg ggaataaacg caaagcggcc agcaacagaa cttgtagcca
     5401 tctgtgccgc attcacatca gacgggcggt ctgcggcatt aaataacttc aggcgttgat
     5461 gtgctgccag ctctgcccca accagtacaa ccagacgagg atcttcacga aactgctcag
     5521 caataagctc tgtaatcaga tggttggcca gagcatcaat atttttccag gtgccagtct
     5581 ctcccagagt gacaggctca gagatgattt gctttccgtt cccgtaggct ttcgccagtg
     5641 catgccagcc aatattgaca tcctcgcctt ttttattgat ttccgggttt gtagtatttg
     5701 caatcgacac accgttgaaa ccgacccgca gcatgtccag cgcatatgcc tgggcaaaaa
     5761 agctctcaac cgttttctca aactcatccg gcacgccggt atggtaaata tgagacatat
     5821 cggaatagct gatccgggcg caggtatccg tctctgtcag aaagaactcc gtaccattga
     5881 ttgccatttt tttgtggaaa cggccaccat ccacacgtcc ggtatacagt tcactggcac
     5941 caatattgac ggcattacct gacacggcgt taacatcacg tagggtaata tttttaataa
     6001 gccacccgga ttccagaatt gaggtacgca gtatattttc agtaggttcg cttaatgcaa
     6061 agcgtctgtt tgattcttcc tgatagttag tgccagtgac gaatttatta tgataacgac
     6121 gtgctgcgtc cggcctttca gttttgaagg catacatagt ttttcacctt tttaagtatt
     6181 taagaaaaga t
//
LOCUS       DACADF010000218         6175 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.220, whole genome shotgun sequence.
ACCESSION   DACADF010000218 DACADF010000000
VERSION     DACADF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.220"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            158..871
                     /locus_tag="ISB30_004493"
     CDS             158..871
                     /locus_tag="ISB30_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992054.1"
                     /translation="MRLKALAFLVPLEKISWIAGSERACLFSWYLLMQFIQNNRAKIS
                     ADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEWIIQTKDRWIR
                     AFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHAIHLSFDIWVT
                     CPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLKELSRVRRLST
                     GEMLHDLIVEEYKRYRHSR"
     gene            956..1828
                     /locus_tag="ISB30_004494"
     CDS             956..1828
                     /locus_tag="ISB30_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAP1992055.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRNRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            2201..5047
                     /gene="ag43"
                     /locus_tag="ISB30_004495"
     CDS             2201..5047
                     /gene="ag43"
                     /locus_tag="ISB30_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HAP1992056.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAMATDTVVNTGAEGGPDADNGDTGQFVRGNAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVTGSNRLGNFTVE
                     NGNADGVVLESGGRLDVLEGHSAQNTLVDDGGILAVSAGGKATDVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGN
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSAK
                     HSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARI
                     RENITLGVQGGYAHSVSGSSAEGYNGQATLNVTF"
     gene            5118..5276
                     /locus_tag="ISB30_004496"
     CDS             5118..5276
                     /locus_tag="ISB30_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAP1992057.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            5431..>6175
                     /locus_tag="ISB30_004497"
     CDS             5431..>6175
                     /locus_tag="ISB30_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAP1992058.1"
                     /translation="MRLASRFGYTNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSPGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPTRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIH"
BASE COUNT         1499 a         1595 c         1718 g         1363 t
ORIGIN      
        1 tgcgtattac tcttattatc ttggagagcg taatatctcc gtactgcccc ttaatgttta
       61 tgatcccccg gaacgtttta tcgcttacat aaaaaaaaac agagaaaatc tgaacatcac
      121 actttcagat tttgaacttg aacaaataat ttctggtatg cgtctgaagg ccctggcctt
      181 cctggtcccc ctggaaaaaa tatcctggat cgcgggcagt gaacgcgcat gcttattttc
      241 ctggtatctg ttgatgcagt ttatccagaa taacagggca aaaataagcg cagacttgct
      301 tcaaaagaat aaactgtatc ttaaagaaga gtatctggaa gggaatgcct tcccgtcgga
      361 ctcatcgaca cagttcagac aaatactcag agttctcgat attctttctg ataagaattt
      421 gcgggatgag tggataatcc agaccaaaga cagatggata cgggcattta aatcaaagag
      481 tcctttcagt tacctcctcc cggagaacga acatgaatgc atatggacat ggaattattt
      541 gaagggaaaa aatattgcac tggaaaaact ggccagtttt cctggttctg ctgatattta
      601 ccacgcaatc cacctttcat ttgacatatg ggttacctgt ccactcacct cacctgacga
      661 tataaaaaat ttcagaaaca gtttcaataa ggccaaggcg cagcgcaagt acaaaaaaat
      721 gcaggaagat aaagtcaatg tacagttttt tcttgacgca gaaaccaggg cgcagctcaa
      781 agaattatcg agggtcagaa gactcagtac cggcgaaatg ctgcatgacc tgattgttga
      841 agagtataaa aggtaccgac attccagata atttcagaca tatattatct ccgtgagtcc
      901 cccgccacct ttccggtgtg cgggggtttt gtcttttttc accgggaata catgtatgaa
      961 tccgtctgat gccattgagg caattgaaaa accgctctcc tccctgcctt actcgctttc
     1021 ccgtcacatc ctggaacatc tgcgcaaact caccagtcac gaacccgtga ttggcattat
     1081 gggtaaaagc ggggccggta aatcctcact ctgtaatgca ctgtttcagg gggaggtcac
     1141 cccggtcagt gatgttcacg ccggcacccg ggaagtacgg cgcttccgtc tgagtggcca
     1201 tggtcacagc atggttatca ctgacctgcc cggggtgggc gagagccgga acagggatgc
     1261 agagtatgaa gccctgtacc gtgacattct gcctgaactt gacctggtac tgtggctgat
     1321 taaagccgat gaccgtgccc tgtctgtgga tgagtatttc tggcgacaca tcctgcaccg
     1381 gggacatcag caggtgctgt ttgtggtgac gcaggccgac aaaacggagc cctgccatga
     1441 atgggatatg gccggcattc agccttctcc cgcacaggca cagaacattc gggagaaaac
     1501 ggaggcggta ttccgtctgt tccggcccgt acatccggtt gtggccgtat cggcccgcac
     1561 cggctgggaa ctggatacgc tggtcagtgc gctcatgaca gcacttcccg accatgccgc
     1621 cagtcccctg atgacccgcc tgcaggacga gctgcgcacg gaatctgtcc ggtctcaggc
     1681 ccgtgaacag tttaccggtg cggtggaccg gatatttgac acggcggaga gcgtctgtat
     1741 tgcctctgtt gcacgcacgg ttctgcgtgc cgtccgtgac acggtggtct ctgttgcccg
     1801 cgctgtatgg aactggattt tcttctgaac actctgccag gggaaatgcc tgattacatt
     1861 gcgttaacca actgaaaaca tgatacaaac actccatccc cctcctgttt ctcttgcgtg
     1921 actgctctgc tgttaataga ataaaacgat cgataaaaca ggtatcgatc gttaccatcg
     1981 atcgtttata gtggttaatg gtctgcgaaa aggcagctga atctcttcat catgcagaac
     2041 ggaattgcac acaacggact gatacttctc tggcggatga gagaggggaa gcatttatgt
     2101 ccgggaaaaa ccactttatc tgaccatgct gttttgtacc tgccggtatc cacttttgtg
     2161 ggtaccggct tttttattca ccctctgtaa ggaaaagctg atgaaacgac atctgaacac
     2221 cagctacagg ctggtatgga atcacattac gggcaccctg gtggtggcct ccgaactggc
     2281 ccgctcacgg ggaaaacgcg ccggtgtggc ggttgcactg tctcttgctg ctgtcacatc
     2341 agtcccggca ctggctgctg acacggttgt acaggcggga gaaaccgtga acgacggaac
     2401 actgacaaat catgacaacc agattgtcct cggtacggcc aacggaatga ccatcagtac
     2461 cgggctggag tatgggccgg ataacgaggc caataccggc ggacaatgga tacaaaatgg
     2521 cggtatcgcc aacaacacta ctgtcaccgg tggtggtctt cagagagtga atgccggagg
     2581 aagcgtttca gacacggtta tcagtgccgg aggaggacag agccttcagg ggcaggcagt
     2641 gaataccact ctgaacggcg gtgagcagtg ggtacatgaa ggcgggattg caacgggtac
     2701 cgtcattaat gagaagggct ggcaggccgt caaatccggc gcaatggcaa ccgacacggt
     2761 tgtgaatacc ggcgcggaag ggggaccaga tgcagacaat ggtgataccg ggcagtttgt
     2821 tcgcggaaat gccgtacgta ccaccatcaa taaaaatggt cgtcagattg tggctgctga
     2881 aggaacagca aataccactg tggtttatgc cggcggcgac cagacggtac acgggcatgc
     2941 gctggatacc acactgaatg gcggttacca gtatgtgcac aacggaggca cagcctctga
     3001 cacggttgta aacagtgacg gctggcagat tgtcaaggaa ggtggtctgg cggatttcac
     3061 caccgttaac cagaaaggca aactgcaggt gaacgccggt ggtacagcca cgaatgtcac
     3121 cctgaagcag ggaggcgcac tggtcaccag tacggcggca accgtcaccg gcagcaaccg
     3181 tctgggcaat ttcactgtgg aaaacggtaa tgctgacggt gttgttctgg agtccggtgg
     3241 tcgcctggat gtactggaag gccattcagc gcagaatacc ctggtggatg acggcggtat
     3301 cctggcagtg tctgccggtg gtaaggcaac agatgtcacc atgacatccg gtggtgccct
     3361 gatagcagac agtggtgcca ctgttgaggg gaccaatgcc agcggtaagt tcagtattga
     3421 tggtatctcc ggtcaggcca gcggcctgct gctggaaaat ggcggcagct ttacggttaa
     3481 tgccggagga caggctggca acaccactgt cggacatcgt ggaacactga cgctggctgc
     3541 cgggggaaat ctgagtggca gaacacagct cagtaaaggt gccagcatgg tactgaatgg
     3601 tgatgtggtc agtaccggcg atattgttaa cgcaggggag attcgctttg ataatcagac
     3661 gacaccggat gccgcgctga gccgtgctgt tgcaaaaggc gactccccgg taacgttcca
     3721 taaactgacc accagtaacc tcaccggtca gggtggcacc atcaatatgc gtgttcgcct
     3781 tgatggcagc aatacctctg accagctggt gattaatggt ggtcaggcaa ccggcaaaac
     3841 ctggcttgcg tttacaaatg tcggaaacag taaccttggg gtggcaacct ccggacaggg
     3901 tatccgggtt gtggatgcac agaatggcgc caccacagaa gaaggtgcgt ttgccctgag
     3961 tcgcccgctt caggccggcg cctttaacta caccctgaac cgtgacagcg atgaagactg
     4021 gtacctgcgc agtgaaaatg cttatcgtgc tgaagtcccc ctgtatacat ccatgctgac
     4081 acaggcaatg gactatgacc ggattctggc aggctcccgc agccatcaga ccggtgtaaa
     4141 cggtgaaaat aacagcgtcc gtctcagcat tcagggcggt catctcggtc acgataacaa
     4201 cggcggtatt gcccgtggag ccacgccgga aagcagcggc agctatggct tcgtccgtct
     4261 ggagggtgac ctgctcagaa cagaggttgc cggtatgtct ctgacgacag gggtgtatgg
     4321 tgccgcaggc cattcttccg ttgatgttaa ggatgatgac ggctcccgcg ccggcacggt
     4381 ccgggatgat gccggcagtc tgggcggata cctgaatctg gtacacacat cctccggcct
     4441 gtgggctgac attgtggccc agggaacccg tcacagcatg aaagcgtcat cggacaataa
     4501 cgacttccgc gcccggggct ggggctggct gggctcactg gaaaccggtc tgcccttcag
     4561 tatcactgac aatctgatgc tggagccaca actgcagtac acctggcagg gactctccct
     4621 ggatgacggc caggataacg ccggttatgt gaagttcggg catggcagtg cacaacatgt
     4681 gcgtgccggt ttccgtctgg gcagccacaa cgatatgagc tttggtgaag gcacctcatc
     4741 ccgtgacacc ctgcgcgaca gtgcaaaaca cagtgtgagt gaactgccgg tgaactggtg
     4801 ggtacagcct tctgttatcc gcaccttcag ctcccggggt gacatgagca tggggacagc
     4861 cgcagccggc agtaacatga cgttctcacc gtcccggaat ggcacgtcac tggacctgca
     4921 ggccggactg gaagcccgta tccgggaaaa tatcaccctg ggcgttcagg gcggttatgc
     4981 ccacagcgtc agcggcagca gcgctgaagg ttataacggt caggccacgc tgaatgtgac
     5041 tttctgataa tcaggtcact ctccttcagg ccccggtcat catgaccggg gctttttact
     5101 ttcagggagc aacagatatg ccgggttgta catcacgttt attgcccgaa ggccctttct
     5161 cacgcgaaca ggctgttgcc gtcaaaacag cttaccgcaa tgtgtttact gaagatgacc
     5221 agggaacata ttcccggctg gttatccgta atgctgaagg tcagttgcgc tggtgatgcc
     5281 ggaacacagc ttaatactca gctcgccagt taagggcttc tcagacaata aacgtcattc
     5341 atttcatctg tcaggccgcg tcttctccgg gagacgtggc tttttcattt attacaccag
     5401 taaatcttaa ccaccgataa ggagcaaatt atgcgactag ccagtcgttt tggttatacg
     5461 aaccagatac gccgtgaccg tccgctgaca cacgaagaac tgatgcacta tgttcccagt
     5521 attttcggtg aagaccggca tacttcccgc agtaaacgat atgcgtacat tcccaccatc
     5581 accgtactgg aaagcctgca gcgggaaggc tttcagccat tcttcgcctg ccagacccgt
     5641 gtgcgcgacc cgggccgccg gggatacaca aaacacatgc tgcgtctgcg gcgggccgga
     5701 gagataaacg gagaacatgt ccctgaaatc attctgctca actcccatga cggtacctcc
     5761 agctaccaga tgctgccggg ttacttcagg ttcgtctgtc agaacggatg cgtctgtggt
     5821 cagtctccgg gggaagtgcg tgttccgcat cggggaaatg tggtggacag agtcattgaa
     5881 ggggcttacg aggtggtggg cgtgtttgac cggattgagg aaaagcgtga tgccatgcag
     5941 tcgctggtcc tgccgccacc gacacgccag gcgctggcac aggcggcact gacttaccgt
     6001 tatggtgacg aacatcagcc agtcaccacc gctgacattc tgacaccacg acgccgggag
     6061 gattacggta aagacctgtg gagcgcatat cagaccatcc aggagaatat gctgaaaggc
     6121 ggaatttccg gccgcagtgc aaaaggaaaa cgtatccaca cccgtgccat tcaca
//
LOCUS       DACADF010000219         6109 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.221, whole genome shotgun sequence.
ACCESSION   DACADF010000219 DACADF010000000
VERSION     DACADF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.221"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            1..165
                     /locus_tag="ISB30_004498"
                     /pseudo
     CDS             1..165
                     /locus_tag="ISB30_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            126..245
                     /locus_tag="ISB30_004499"
     CDS             126..245
                     /locus_tag="ISB30_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP1992059.1"
                     /translation="MPLSYTGYHHFIMMSLMSGEHNTFALSLQDESPGLVPAG"
     gene            280..622
                     /locus_tag="ISB30_004500"
                     /pseudo
     CDS             280..622
                     /locus_tag="ISB30_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(989..1255)
                     /gene="minE"
                     /locus_tag="ISB30_004501"
     CDS             complement(989..1255)
                     /gene="minE"
                     /locus_tag="ISB30_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAP1992060.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1259..2071)
                     /gene="minD"
                     /locus_tag="ISB30_004502"
     CDS             complement(1259..2071)
                     /gene="minD"
                     /locus_tag="ISB30_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAP1992061.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2095..2790)
                     /gene="minC"
                     /locus_tag="ISB30_004503"
     CDS             complement(2095..2790)
                     /gene="minC"
                     /locus_tag="ISB30_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAP1992062.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKSPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3310..3678
                     /locus_tag="ISB30_004504"
     CDS             3310..3678
                     /locus_tag="ISB30_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992063.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VSSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3781..4182)
                     /locus_tag="ISB30_004505"
     CDS             complement(3781..4182)
                     /locus_tag="ISB30_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAP1992064.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4423..4716
                     /locus_tag="ISB30_004506"
     CDS             4423..4716
                     /locus_tag="ISB30_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992065.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4788..5447
                     /locus_tag="ISB30_004507"
     CDS             4788..5447
                     /locus_tag="ISB30_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAP1992066.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKVGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5524..5985
                     /locus_tag="ISB30_004508"
     CDS             5524..5985
                     /locus_tag="ISB30_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAP1992067.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKGLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT         1563 a         1354 c         1495 g         1697 t
ORIGIN      
        1 agcaacgatt acattagtca ttttattttg tggacggcct cattgtcgaa aaataagcgt
       61 acgacagtat tatcagaaaa gagtgttttt tatccaacaa cacttcagcg cactgcgttt
      121 aaaaaatgcc tctttcttat acgggatatc atcatttcat catgatgtct ttgatgagcg
      181 gtgaacacaa tacatttgcg ctgtctcttc aggatgaatc ccctggtctg gtgcctgcgg
      241 gctgatgttg cagcagagct aaggtcactt agacgctact atcatttatc caatgcatgg
      301 aatcgaaatc agtcgatacc cgcagtatat atcgtgaact gggcgcaacg ctgagctaca
      361 acatgcgtct ggggaacggt atggaaattg aaccctggct gaaggcggct gtgcgcaaag
      421 aatttgtcga tgataaccgg gtgaaggtga atagtgacgg taattttgtc aatgatttgt
      481 cgggcagacg tggaatatac caggcaggta ttaaagcctc attcagcagt atgttaagcg
      541 ggcatctcgg ggtgggtata accgtggtgc cggtgtggag tccccgtgga atgcggtggc
      601 cggtgtgaac tggccgttct gaccatcaac gattaactgc gtttcggcgc tgttttcgtt
      661 tacagggtgc taaaagggaa aattctgggg caaaaaaagc ccgccagtta cggcgggaaa
      721 cctcatccta ttggagaaca atgaataatg aaattgcggg ttatcatctc cctggatacc
      781 catgctaaca ataaggttat ttactcaacc aggcgtaaac attttgttct gtgcgtggga
      841 acagccttaa ggtgtaaagg ggggggaaat agcaatgagt agtatcagca agaatactcg
      901 ccgctttacc acaacgtgga tgagagggat gaaaaactca aggcagagat gactctgcct
      961 tgaagataaa tgcgctttta cagggggctt atttcagctc ttctgcttcc ggtaaggtca
     1021 cgttcagctc aagaatagaa atatcgccat ctttttgctc aagctgtacg gttaccatct
     1081 caggatcaat ttgtacgtat ttacaaatga cctcaagaat atctttacgc aactgcggca
     1141 gataatgcgg ttctgcatcg ctgcgacggc gttcagcaac aataatctgc aaccgttctt
     1201 ttgcaatgtt ggctgtgttt ttcttccgcg agagaaagaa atcgagtaat gccataactt
     1261 atcctccgaa caagcgtttg aggaagcctt tcttctcttc ttcaatgaag cggaaaggac
     1321 gttcttctcc caacagacgt tctacggtat ctgcgtaggc tttacccgca tcggcgttaa
     1381 tgtcgagaat gaccggttca ccctggttag aggcgcgcaa tactgattga tcctctggga
     1441 tcacgccgac gagtttgatg cgcaggatct ccagcacatc ttccatgctc agcatgtcac
     1501 ctctgcttac gcggcctggg ttatagcgcg ttaacagcag gtgctcttta atcggctctt
     1561 cgccattttc tgcgcggcgt gatttcgacg ccagaatgcc taaaatacgg tcagagtcgc
     1621 gtactgagga gacttccggg ttggtggtaa taatggcttc gtctgcaaaa tagagtgcca
     1681 ttaacgcacc ggtttcaatc cctgccgggg agtcacaaac gataaattca aaatccatcg
     1741 ctttcagatc atcaagaact ttggcgaccc cttcacgggt gagggcatct ttatcgcgag
     1801 tttgcgatgc cggaagaata tagagatttt cagtacgttt atctttaatt aacgcctggt
     1861 ttagcgttgc atcgccctga atgacgttga cgaaatcgta aacgacccgg cgttcacaac
     1921 ccataatcag gtcgagatta cgcaggccga tatcaaaatc tatcacgaca gttttctttc
     1981 ccttctgggc caaaccagtg gcgatggccg cgctggaggt tgtcttacca acaccccctt
     2041 tgcccgaagt aacaacaata atgcgtgcca tagaaattcc ttgttaaaaa gggatcaatt
     2101 taacggttga acggtcaaag cgttttcgac taactgcagt cgtgccgctt tgccataaaa
     2161 ttctgctggg atttgatcac tcagccagta ttcacctgcg atggacacca gttccgccat
     2221 caggttcgta caaaatattt gcgtttcccg gtcaccactt gcccctgcca gcgcacgacc
     2281 gcgcatcatg ccatagacat gaatgttacc atcggcaatc aattcggccc ctgcgctaac
     2341 gtggcttgta acaatcagat cacattgtgg agcataaata cgctgaccgg aacgcactgg
     2401 ggtatcaatt aaacgcgttt ttgtgaccgg cgttgtattt tgcgctggag cctgcggtgc
     2461 gggggctgga cgcggcgatt tttcctttcc ttccgtcagg ataggcagcc ccatcttttc
     2521 aatttcggct ttaagttgcg catctttgca gccacttacg ccaataaccc gcaaaccagt
     2581 tgccgaaacc gccttatgca tcgccgacca gtttaccggg tcttccagtg cactgacgtt
     2641 gagtacaacg ggggcatgtt ttaaaaatgc gggggcctga gcgattttgt cttccagcgc
     2701 ctgatggata accttaggtt ctgcctcatg cagatgaacc acagataaag tgaagctact
     2761 gcctttaagc tcgattggcg tgtttgacat cctggcctta ctcaattagc tattaatcat
     2821 cgccagcgcg cgatgatgtt ccgaagacta taaggcatgt tatagtctgg attatattga
     2881 ggcaagtcac cctcccattt attcagagta aaagtctatt ctgtgataaa tggcgttgat
     2941 tcatagctta aaaaataccc ttgtcaatca acccattgcc gtcgtacttt tgattgttct
     3001 tatttacgct tcctttttcc gcaccctaat cgtgacagca tcttcagacc tgtttaaata
     3061 tccagtattg aggcgagtgt attttttcac cacgccaggc tttcttaact ttccattttt
     3121 tgcagcatca ttaaagaaca gatacccgtt atcttaatat ccgccgcaga attgctctat
     3181 catagaaacc gcagaagacg tctgatactt ctgtcaggtc atcgtaactc gtccaggtca
     3241 gcggttttga ttacggattg atgatgaaat gagcataagt gttgtacggt tggattatca
     3301 ggatgtgcta tgaatatgat gcgtattttt tatatcggat tgtcaggtgt ggggatgatg
     3361 ttctcatcaa tggcgtctgg aaatgacgct ggcgggcttc aatctcctgc gtgtggcgtt
     3421 gtttgcgacc cgtatatttg tgtgagttca gatggcattt ccccagagtt aacaaggaaa
     3481 tatctcggcg aaaaagccgc tgaaaactta caatcattac aaggctacga tcccagcgaa
     3541 tttacattct ctaacggtgt attttgcgat gttaaagaaa aattatgtcg tgatgatcgt
     3601 tattttggtg tggatggtaa gcggagtgga aaaatcaatc aaaccaccac aaagatgtta
     3661 tttatgtgtc gtgagtgatg tcttgtttac attatgttct attcgtcatt aacctcttaa
     3721 gcgacagaga ggagtgtggc gttaacgttt atgacgggga gagtccccgt cggttgacat
     3781 ttacttaatt tgaatatcga cgttatattt tttagttttg tttttacggg cgtcgtttcg
     3841 ggtttgcagc actcgcagct catatttgcc agaagcaggg agtgagtatt gtccgtgact
     3901 atcgagttct ggggaatatc tggataaatc aacagaatca tcgattcctg gcccgaagag
     3961 gtaagtatca gcgccttcat tagagatact cacatgcact ttctgacctt ttttggcata
     4021 gaaggtatat gtatcgtaat cgtatccctt tatttcgccg gagtactgag cgctactatg
     4081 gccttttctg aactcaacat tgacattctt acccgcagca aagctcgtgg aagttagcag
     4141 tgcaagcaac aatacagcct tactgatgcc cttaattttc attggtagtc tcctgttcag
     4201 aatgagtacg atgaactgtt ataatataac aatccttcac gggagaattc cagtggcttt
     4261 cctgtgttcg gttaaatcct ggcgcggcat ttttgccggg ggattactta taaagggggc
     4321 atggactgaa caaaccgtag aaatctacag ctggctggct tgtggcgcgg gtttcattga
     4381 tgttagctta tgccaaaacc tggcatctta aagagtaaaa gtatgttttg tgtgatttat
     4441 cgaagcagca aacgtgacca gacctattta tatgtcgaaa aaaaagacga tttttcgcgt
     4501 gttcctgagg aactgatgaa aggttttggt cagcctcagt tagcgatgat tcttccgctg
     4561 gatgggcgga agaaactggt caatgccgat attgaaaagg ttaaacaggc attaaccgag
     4621 caaggttact atttgcagtt accgccacca cccgaagatt tgctgaagca acatctttcc
     4681 gtcatggggc agaaaacaga cgacactaac aaataaccga tatccggcgg tggcattatc
     4741 tttgtcggcg cgggttttca tatccacgat aaggtgaggg gaacgttatg tatcaacatc
     4801 acaactggca aggtgcgctg ctggattatc cggtgagtaa agtagtctgt gttggcagta
     4861 actatgccaa acatattaaa gagatgggca gcgcagtacc cgaagagcca gtgctgttta
     4921 ttaaaccaga aacggcactg tgcgatctgc ggcagccatt ggcgatccca tccgatttcg
     4981 gttcagttca tcatgaagtc gaactggcgg tgttgattgg cgcgacgctg cgtcaggcta
     5041 cggaagagca tgtccgcaaa gccattgccg gttacggcgt ggcgctcgat ctgacattgc
     5101 gtgatgttca gggaaaaatg aagaaagccg ggcagccgtg ggaaaaggcc aaagcgtttg
     5161 ataactcttg tccgctttcc gggtttattc ccgcggcgga atttaccggc gacccgcaaa
     5221 atacaacgct gggcctgagc gtaaacggcg agcaacgcca gcaaggtacg actgcggaca
     5281 tgatccataa aatcgttccg ctgatcgctt atatgagcaa gttttttacc ctcaaggtcg
     5341 gtgacgttgt gctgacaggc acgcctgatg gtgtcggccc gttgcaaagc ggtgatgagc
     5401 tgacagtcac tttcgatggg cattctttga caactcgcgt tttgtaatac tttttgccgc
     5461 ctgaaagcgg cggcaaaact tgcatcgctg tgccagactg gttataaggt gcgttttaac
     5521 gtaatggcgg aacacctgat gagcgatgta cctttctggc aaagtaaaac cctggacgaa
     5581 atgagcgatg cagaatggga gtcgttgtgt gatggttgcg gtcagtgttg cctgcataaa
     5641 ctgatggatg aagacaccga cgaaatctac ttcactaacg tcgcctgtcg ccagctcaat
     5701 attaaaacct gtcaatgtcg gaactacgaa cgtcgtttcg agtttgaacc cgactgcatt
     5761 aaattaaccc gtgaaaatct gccaacattc gaatggctgc caatgacctg cgcttatcgt
     5821 ttgctggcgg aaggtaaagg tttacctgcg tggcatccgc tacttactgg ttcgaaagcg
     5881 gcaatgcatg gtgaacgtat ctctgtgcgt catatcgcag tgaaagaatc agaagtgatt
     5941 gactggcagg atcatatctt aaataaacct gactgggcgc agtgataaat ttaatagatg
     6001 ttgatggggg ctttcttaat atacgtctgg atgctcgcca tttcgtagcc ctgtttttga
     6061 aagcgctctg tcataatgag agttacccga tactttatta tcaacaggt
//
LOCUS       DACADF010000220         6103 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.222, whole genome shotgun sequence.
ACCESSION   DACADF010000220 DACADF010000000
VERSION     DACADF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.222"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(74..994)
                     /gene="speB"
                     /locus_tag="ISB30_004509"
     CDS             complement(74..994)
                     /gene="speB"
                     /locus_tag="ISB30_004509"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAP1992068.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(1130..1861)
                     /locus_tag="ISB30_004510"
     CDS             complement(1130..1861)
                     /locus_tag="ISB30_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992069.1"
                     /translation="MKKWKLRSALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARIGMNYQLYIRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVHNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(2007..3983)
                     /gene="speA"
                     /locus_tag="ISB30_004511"
     CDS             complement(2007..3983)
                     /gene="speA"
                     /locus_tag="ISB30_004511"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAP1992070.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(3992..4123)
                     /gene="yqgB"
                     /locus_tag="ISB30_004512"
     CDS             complement(3992..4123)
                     /gene="yqgB"
                     /locus_tag="ISB30_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAP1992071.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            4259..4474
                     /locus_tag="ISB30_004513"
     CDS             4259..4474
                     /locus_tag="ISB30_004513"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992072.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLFSTRLEVATRKKRRVLFI"
     gene            4778..5932
                     /gene="metK"
                     /locus_tag="ISB30_004514"
     CDS             4778..5932
                     /gene="metK"
                     /locus_tag="ISB30_004514"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP1992073.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1543 a         1653 c         1498 g         1409 t
ORIGIN      
        1 tgtaggtcgg ataaggcgtt cacgccgcat ccgacattaa tgacacatca cccgtgcgcc
       61 tcttatctgg cgcttactcg ccctttttcg ccgcctgaat atacagcatc tccagcgcca
      121 gcgtcgccgc agccagagcg gtgatttccg actgatcgta tgccggagcc acttccacta
      181 cgtccatccc aacgatgttg agatctttca ggccgcgtac cagtttaatg gcgcgatcgg
      241 aggtcaggcc gccaattacc ggcgtaccgg tgcctggtgc aaaagcagga tccaggcagt
      301 cgatatcaaa ggtcaggtaa accggcatat cacccacaat ctgtttcacc tgggcgataa
      361 tgtcatccac gctgcgatcg ttcacctggc aggcgtccag cacggtaaag ccgttgtctt
      421 tatcaaactc ggtacgaata ccaatctgca cggaatgatt cgggtcgatc agaccttctt
      481 tcggcgcggt atagaacatg gtgccgtggt caaattcaca accgttcgca taggtatcgg
      541 tgtgggcgtc aaagtgtacc aacgccattt tgccgaaatg cttcgcatga gcgcgcagca
      601 gcggcagcgt aacaaagtgg tcaccaccga aggagagcat acgcttaccg gcagccagca
      661 gcttctcggc gtgcgcctgc agcttctcgc tcatctcacg ggcatcgccg aaggcatata
      721 ccagatcacc gcagtccacg acgttcaggc gctcacgcat gtcgaaattc cacgggaagc
      781 ggttgtgttc ccaggccaga ttcgtcgaaa cctgacggat cgctgccgga ccgtgacgac
      841 cacccgcacg accagaagtg gccatatcga acggcacgcc agtaatcacc cagtcagcat
      901 cgctgtcata cggctggaag ttcatcggca ggcgtaaaaa accaaaggcg ttggaaacca
      961 gtgagttatc gtattgatga cctaaggtgc tcatggatgg actcctttta caaagtcgat
     1021 ataaaaaaac ccttccgcgt cgttaggccc gacgaggaag ggttggattt gcgaaacaca
     1081 agtttgtgct aattatcgcc ttaaatgggc gaggcatcaa gcgaggttgc tatttccgac
     1141 acggtggtgg cgcatagcca ttgttatctg ccggaaacgc ttgtccagag cacggcggca
     1201 gcgcgttttg cgccgggcct tgtaattcaa aggcggcaaa gccgattaca ccggtattat
     1261 gcacatcgcc ttgcgccgaa ttggctgcgt aggaatcagc aacgttggcg aactgaaaag
     1321 ccgcttcggt atgtttatct ttgcggaatc ctttaatcgc taacgaatct ccggcattca
     1381 caatataacc gttgttattc agcgaacctt gcttgccgtt cagcacatcc aggccatcaa
     1441 ccgtagcaac gatctcgtag ttggtgttac gactgtaatt acggatatat aattgataat
     1501 tcattccgat tctggcatgg acaatatagc gtggttcatt attggatgaa tcataaaccc
     1561 gcgtaatagg tagagaatta aaattaccat cgcgaatagc atattccagt tcgttaatac
     1621 gaatactata aacgcggtcg tagccggaag gaacattagt cgaataacta ataacgatca
     1681 tctctgccgg atcgcgccag gctcgttcag cagatacgcc ctgaaccgtt gagtgcacat
     1741 ccccacccca ggcggtgccg acattacccg atgcactgct attatactgc gcattactgg
     1801 aacaaccggc cagcagcacg gctaatgcta tcaacgcgct acgtaatttc cattttttca
     1861 taaccgttcc ctctgcattt aattaatgca gcgattgtag ggagattatt taataagaaa
     1921 agcagctaaa ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
     1981 tcgccccgcc agcggaataa tcttaattat tcatcttcaa gataggtata accgtacaaa
     2041 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
     2101 acctgatcgc ggaactgagt taacagcgtt ttcggatcga gctgcacata ttgcagcatg
     2161 tccgccacgg tatcgccttc gtcagacagt tctacctcta cgctaccatc agggaagacg
     2221 aacacgtcaa ccgcttcggt atcaccgaac aggttgtgca tgttgccaag gatctcctga
     2281 tatgcgccga ccataaagaa accgagcatc ggcggattct ccggatcgta ctccggcata
     2341 ggcattgtcg tggcaatacc gtcaccatca atatagtggt cgatagcacc gtctgagtca
     2401 caggtaatat ccaacagcac agcgcggcgt tccggcactt gatccagccc ttccagcggc
     2461 agaaccggga acaactggtc gatcccccac gcatccggca tcgactggaa cagcgagaag
     2521 ttgacgtaca ttttgtccgc catacgttcc tgcagctcgt cgataatcgg acgatgagca
     2581 cggttttgcg gatccagctg cttttgcact tcatggcaca tgctcaaata aagctgctca
     2641 gcccatgcgc gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatatcgtgc
     2701 agatccatct ggctgtcgtg taaccattca cgcagagaac ggcgagttcc cggttcgtgc
     2761 atttcctgcc aggtttccca catgctttgc agggcgcgcg gtgcgtcttc tgcaggcgcg
     2821 gtcggcaccg tgtattcgtt acgttccacg ccgatgatat tcgacaccag cacggtgtga
     2881 tgcgcagtca ccgcacgacc cgattcggtg attaccgtcg gatgtggcag accattttct
     2941 tcacacgcat caccaatcgc ccagatgatg ttgttggcgt attcattgag gccgtagttc
     3001 accgaacagt cggactgcga acgggtgcct tcatagtcca cgcccagacc gccgccgacg
     3061 tcgaagcact gaatattgac gcccagcttg tgcagctcaa catagaaacg cgcggattca
     3121 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagc
     3181 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     3241 gtcgccgcca ggccgaactt cgatttttcc ccgccggagg actgccattt accggagccc
     3301 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     3361 tccagcacaa tggcaatttc tgacatcttc tcaatgacca gatagacctt gtggcccatc
     3421 ttctcgccaa ttaatgccag acggatatat tcgcggtcct tataaccgtt gcagacgatg
     3481 acgctacggg tcatgccagc atgtgccagc accgccatca actcagcttt ggaaccggct
     3541 tccaggccca gcggttcgcc cgaatgaatc agggactcaa tcacgcggcg gtgctggtta
     3601 actttgatcg gataaacgag gaagtaatcg ccgttatagc cgtaggattc ccgcgcacgt
     3661 ttgaacgcgg cgttaataga acgcaaacgg tgctgcagga tctgcgggaa acagaacagt
     3721 gcaggcagac gctggccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     3781 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     3841 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     3901 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     3961 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     4021 taacgactat cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     4081 gtgaatgctg acgctccaac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     4141 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     4201 cgcccttgtt cagtcctcag tagctgtaac cagctcttga atcctgagaa gcgccgagat
     4261 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gaatcttcac
     4321 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     4381 gccgcctgtt aagacgaacc cacaagccaa aactctgttt tcaacccggc tggaagtggc
     4441 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     4501 aatggcagaa aattgcaaaa gataaatacg cagaatgcca gcattgtcag gaaaaatttc
     4561 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatt gggtgaagaa
     4621 ctgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     4681 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     4741 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     4801 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     4861 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     4921 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     4981 cacccgtaac accgttcgcg aaattggcta tgtacattcc gacatgggct ttgacgctaa
     5041 ctcctgtgcg gttctgagcg ccatcggcaa acagtctcct gacatcaacc agggcgttga
     5101 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     5161 caatgaaacc gacgtgctga tgccagcgcc tatcacctat gcacaccgtc tggtacagcg
     5221 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     5281 ggtgactttc cagtatgacg acggcaaaat cgttggtatc gatgctgtcg tgctttccac
     5341 tcagcactct gaagaaatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     5401 caagccgatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     5461 cggtcgtttt gttatcggtg gcccgatggg tgactgcggt ctgactggtc gtaaaatcat
     5521 cgttgatacc tacggcggca tggcacgtca cggtggtggt gcattctctg gtaaagatcc
     5581 atcaaaagtg gaccgttccg cagcgtacgc agcacgttat gtcgcgaaaa acatcgttgc
     5641 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     5701 gacctctatc atggtggaaa ctttcggtac cgagaaagtg ccttctgaac aactgactct
     5761 gctggtacgt gagttctttg acctgcgtcc atacggtctg attcagatgc tggatctgct
     5821 gcacccaatc tacaaagaaa ccgcagcata cggtcacttt ggtcgcgaac atttcccgtg
     5881 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatttttctt
     5941 cacctgcttt taaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     6001 aggcgttcat gccgcatccg gcaaaaagct gcccgcacaa taacatcatt cttcctgaac
     6061 acgttttacc gcaaattatc atcgctactg aaaccgatta cac
//
LOCUS       DACADF010000221         6010 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.223, whole genome shotgun sequence.
ACCESSION   DACADF010000221 DACADF010000000
VERSION     DACADF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.223"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..315
                     /locus_tag="ISB30_004515"
     CDS             <1..315
                     /locus_tag="ISB30_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP1992074.1"
                     /translation="VRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLII
                     DEIGYLPFGQWDQTFAGDAALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEAN
                     PE"
     gene            complement(382..537)
                     /locus_tag="ISB30_004516"
                     /pseudo
     CDS             complement(382..537)
                     /locus_tag="ISB30_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238951.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(652..867)
                     /locus_tag="ISB30_004517"
     CDS             complement(652..867)
                     /locus_tag="ISB30_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992075.1"
                     /translation="MAWNTDVSADGKPHSLWKQGRALASQPESVTKTNIAENRIHRSG
                     VNARRQKPLSAQPPSDLNKKHTHNRLT"
     gene            complement(1341..1943)
                     /locus_tag="ISB30_004518"
     CDS             complement(1341..1943)
                     /locus_tag="ISB30_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992076.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(2037..2315)
                     /locus_tag="ISB30_004519"
     CDS             complement(2037..2315)
                     /locus_tag="ISB30_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAP1992077.1"
                     /translation="MPVKVARCFHDHTGWFNNIRRNILMATPVSLMDDQMVDMAFITQ
                     LTGLTDKWFYKLIKVGGFPAPIKMGRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(2439..3635)
                     /locus_tag="ISB30_004520"
     CDS             complement(2439..3635)
                     /locus_tag="ISB30_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HAP1992078.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLDHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALAVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASVAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQAS"
     gene            4399..4575
                     /locus_tag="ISB30_004521"
     CDS             4399..4575
                     /locus_tag="ISB30_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HAP1992079.1"
                     /translation="MAARNTSGDAGKVNSHTRYKLTLAELEAFNSAVDNRLAELTANF
                     TSACWLQSCHLSVR"
     gene            5215..5784
                     /locus_tag="ISB30_004522"
     CDS             5215..5784
                     /locus_tag="ISB30_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAP1992080.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIEQAWCSALGVDAGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRG"
BASE COUNT         1412 a         1502 c         1674 g         1422 t
ORIGIN      
        1 gtccgtgcag gtatcaaagt tcgcttcaca acagcagcag atctgttact tcagttatct
       61 acggcacaac gtcagggccg ttataaaacg acgcttcagc gtggagtaat ggccccccgc
      121 ctgctcatca ttgatgaaat aggctatctg ccgttcgggc agtgggatca aacgttcgcc
      181 ggtgatgcag cactgacctc agcgatgctg gaccgtatct tacaccactc acatgtcgtt
      241 caaatcaaag gagaaagcta tcgactcaga cagaaacgaa aagccggggt tatagcagaa
      301 gctaatcctg agtaaaacgg tggatcaata ttgggccgtt ggtggagata taagtggatc
      361 acttttcatc cgtcgttgac acattgcctt aatctccgca aagataacct cgtcattccg
      421 gacattctct gccaggcata tgtcgatgta atccataaac gatttcagct taaccctttt
      481 gtggccggtc tttctggctg gcggttcagg ggatgttttc tggttatcgt gtaccacaaa
      541 actgggatat tgccccgata gtcacatttc agattgatcg cggtctgaag atgtggcact
      601 gaggtaacgt gctggtgtca ctggtattgc tgaagttatg gtttttcggg gtcaggtgag
      661 cctgttgtgt gtatgctttt tgttcagatc agaggggggc tgtgcggaca gcggcttttg
      721 tctccgggca ttcacgcctg aacgatgaat gcgattttca gcaatatttg tctttgtgac
      781 actctctggt tgagaagcta atgcgcgccc ctgtttccac agagaatgag gcttgccatc
      841 agccgaaaca tcagtattcc acgccataca gacggcgcct gaccatgtgc tgatgcaggt
      901 aaaatgtttc aactacaact gttcgggttg ctatgcaacg aaacagcgat tctcacgatc
      961 tttaactgca acgtttttcc gcctgaaagc tgactgcctc ctgctgaggt gggaggctat
     1021 gattcagtgc gtttatagtc tccggggtat gcatgtaccg gagcatacca tttctggtgg
     1081 ataacaagcc ggcacattca gcctgcagtg aagatggagg cacctggttg tgaagaacgt
     1141 ttctgcccgt ggctgcttcg catcatcaat ggtattcata cccgctcaga aatgaccggg
     1201 tgctgaagcc ctccgttatc gcgcagtata atgacgtata tgatctgtgg acgagtgagc
     1261 ggcgtgcctg atgtacgccg ctattactgt attttaccac agggctggtt ttctgctgcc
     1321 agccctgtgg ttatcagaca tcaactgtga gcggcatcaa ccgatgtacg gtatgccata
     1381 agcacgtact gatccatcag atttgtcagt ggttcactct gaccctgacg tgcaatgatc
     1441 cattgctcca gatctttttc tgatttgtgt ttcaggtttt tcgtccagtc gtcaaggctg
     1501 tttccggtca ttaagtcaag ccagcagagc cagaccagtt tgagacgtaa ctccgtgccg
     1561 ccaaactgac ggaagaggct gatcagtttg tttgccagag aatgttgctt caggcgctca
     1621 cattcagaca gcaggatccc ttccagcatc ggatgatgaa ccagcaggtg ttcgctggtg
     1681 cataaattca ccaccagatc atcatccagg gacggggagc tccgtttgtc cggaccataa
     1741 atcagccggt gcagactctg gggaacgatg gcaatctccg gtttttcgcc attgaatgac
     1801 acccacccgg gcgtcagatt atggaaatag tagatattcg gatgacacac gccccatgat
     1861 acggcgtgcc gggtgaagcc ggtcagccat ttctggtagt tttcatggag cagctcgtaa
     1921 cgggtctgat actgttgttt cattttcata ttcctcacgg gggataacag gcagcagagc
     1981 gccgccggca ggccggagat tgcgtctgct ttgggcgaag ggttagtggt taaaaattac
     2041 ggacgggact gtgcaatacg cgcctgcagc caggcttcca cttcactttt cagccagcgg
     2101 gagctgcgcc ccattttgat gggggcagga aagcccccaa ctttgatgag tttgtaaaac
     2161 cacttatcgg ttaagccggt cagttgagtg ataaacgcca tgtcgaccat ctggtcatcc
     2221 atcagcgaaa ctggggtagc catgagaatg ttcctccgga tgttgttaaa ccagccggtg
     2281 tggtcatgaa aacagcgcgc caccttcacc ggcataacaa ccggggagaa acgtaaaaaa
     2341 taatcgggaa tctggctgtc cggagataaa cggggggatt gcgatggcag agccgtagcg
     2401 gaatcccggg gggtgcccct attaagttgt caagcatgtt atgacgcctg cggggtataa
     2461 aaagtcccgt cgcgcatcat ggcgaacaga acgtcgcagc gtcgtctcgc cagggcgata
     2521 agcgcctgat tgtgtcgttt tccctgactc attttgcggg tgtagtaagc cctggagagc
     2581 ggatccctga gcgcggcgaa ggccgacagg aacaacgccc gtttgagagc tttattaccc
     2641 cgtcgcgagg gatgctcacc gcgtattgac gagccggatc gccgagttac cggcgcaagg
     2701 ccagcataag cagcgagatg tgcgacagag gcgaaggcgc ggcaggcgac ctcggtgagg
     2761 agtctggctg cggtcctgac accgactccg ggcatactgg tcaggaccgg gtaaagaggg
     2821 tgagcaagaa ctcgctgttc taccgcaagc gccacctcgt ctctttgctt acgcagcgtg
     2881 atgagctgga gtgccagacg tggcagtact acggcagcgg cattcgtgcc gggaacgacg
     2941 acggtttgtt cggccagtgc ctgagctatg tctgctgcaa ggcgtttacc cagacgaggc
     3001 gcaagtttgc agagctgggc tgccagcttc ttctcaccca gcgaagcgag tttttctggt
     3061 gagggatatc gctggagaag atcgagtacc gccgggtgat caagtctcgg accgagaacg
     3121 cgctccagtg ccggatgtat ctgggtcaga aggccgcgga tacggttgct ggcctgcgtt
     3181 gtctgtgcgg caagatcatc atcgaagccg cagagcatgg agagttcggc gatttgctcg
     3241 tcagccagtt tcagcgtgcg tagcgcgtga ggcagggtac gggcagcttc ggcaatgatg
     3301 gcagcgtcac gagcatcagt tttagcttca ccggcgtgta agtcggctat gcggcgcatg
     3361 gccagtccag ggaggtatcc gacaaggact ccttctgagc gggcaacggc gacaggtaac
     3421 gcaccgatgg tagctggctg atcaacaacc agcagtatct gaccatgttg tttcaggtca
     3481 gatattagcg acctgagttt gttttcgtcg ttgggtaatg ctttatcgaa caggcgttta
     3541 cctgaacgat taatggctac agcgtgatgc gtatctttac cgacatcaac gccgataaag
     3601 acctggacgg attcgtaatc gctggattcg gtcattctgt ctcccttgta tatgggttaa
     3661 gcagataacc acggggagca ggtaccggca tccacgttac agacggtccc ggcaaaagtg
     3721 cctgacctga cccctattag cggttaccag cgccccacca gacccggtga catcaccccc
     3781 cggatcatgg acgactgggg gcagtaatca tgccgggtct ggctggctaa caccccatta
     3841 taaggggtac gaataaagta acgggggtgc gaaaagatgc ttccgcacgc acgcgcgata
     3901 aagaggggat agttttcgtg aggacaggga gagaagcagg cgttctgata attgcagagt
     3961 tgttttcgga ggtggttaca gtgagtcagc ccgttcctgg cgagcaggag actgatatca
     4021 ccgatgggat aaggttgtct gattggtatt ataaatataa gaagtcatca gagagtaacg
     4081 taaaggattt gccagaataa cctaagagag taaatctctc cggtaacaga gcgctgtgaa
     4141 gcattctgta tcagagtgca aatacatgaa cgctgatgcg taaaatcagc agtgacacgc
     4201 cagctccccc tatgcccatt cctgtataag acatgaactg agcgtattaa tgctcctgcc
     4261 ctgagactga gccggtgagt gaggggctga gaaatccctc ttgacactgc tctaaccaga
     4321 aatacttccc cggttgttgc ggtgcgtccg gatccccgat aacggaacac taaggctgta
     4381 ttgcgggaca ccaggtaagt ggcagcccgt aatacgtcag gagacgcggg aaaagtgaat
     4441 tcccacacgc gttataaact tacccttgcg gagctggaag cctttaactc tgccgttgac
     4501 aaccggctgg cagaactgac cgcaaatttt acgagtgcgt gctggcttca gtcttgtcac
     4561 ctgagcgttc ggtaacggag gcgcgggcgg taatagactg attttcaggt attaccggct
     4621 tacggcgtgt ggttgatatt gaggggctgt gagtttcgcg tatttgtcag aaacccagac
     4681 gagttttcct gtatgggacg aaataaggca gcggcaatac ctggaaaaat ttaatatggt
     4741 atgaaggaga acctgtcggt tacgcttaat atacccggta cactgtcagc tcaggggata
     4801 catgaaccgt tatctcacca gctacacagt atatacacac tgacgctgac agtagtcagc
     4861 aggaccacaa taatcagtgc cagcagtatg gtaacgatac ggtctgtatt ccggtaatag
     4921 ccgaaggggt cgacagaacc acaatacggg caacacactg tattatgagt aatcgcgttt
     4981 ccacagtccc tgcacttctt tttgcgtatc accatcactc cctcctcaca gccttacccg
     5041 taaccggttt tttacattaa aaaaacaacg ctgaaaattc aaccgtcagt ccggagacga
     5101 ccgttcgggt tatcacagag tgcctgagac agtgccctgc cggaggtcac ttcagggact
     5161 ctgcgcattt tttacgacgt ggctattccg tatgaaccat acggagattt aaccatgacc
     5221 tacaaataca accccttctg gcagcaacgt attcgtgaga cagtgcggca cgcactgaat
     5281 gttcatcccc gcctgacggc attgcgggtt gacctgcgtt tcccggatgt accggcagca
     5341 acggacgcag ctgtgatatc ccgcttcatc aatgccctga aagcccgaat cgacgcttac
     5401 cagaaacgta agcatcggga aggtaaacgc gtgcatccca ccactctgca ttacgtctgg
     5461 gcccgggagt ttggggagtg caaaggtaaa aaacactatc atctgatgct gctggtcaac
     5521 cgggatacct ggtgtcgtgc cggtgattac cgcgctccgg gatcactggc cggaatgatt
     5581 gaacaggcct ggtgcagtgc cctgggggtg gatgccggat gccatgccac gctggtgcat
     5641 tttccggcct ggccggcggt gtggctggcg cgtaatgatg acaccggctt tcagcaagtg
     5701 ctggaacgtg ctgactatct ggcgaaggag cataccaaag ctcactgcac cggtgagcgc
     5761 aactttggct gcagtcgtgg ctgagccaga cagacgagtt atctgacttc agtgtgatga
     5821 cgtgttcatg gccaccacac tgattttccc ttatgagact gaactgaaac cgccattcac
     5881 acctgaccac gctgcccgta ccgggacggc tgttgcctgc gtgtcgtctg acggtaagga
     5941 acatatacta tgtacgcaaa atcctttctc gctcttgata gcaacggacg tctgacgggc
     6001 gcccgtactg
//
LOCUS       DACADF010000222         6009 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.224, whole genome shotgun sequence.
ACCESSION   DACADF010000222 DACADF010000000
VERSION     DACADF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.224"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            141..869
                     /gene="gadW"
                     /locus_tag="ISB30_004523"
     CDS             141..869
                     /gene="gadW"
                     /locus_tag="ISB30_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAP1992081.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(1232..4345)
                     /locus_tag="ISB30_004524"
     CDS             complement(1232..4345)
                     /locus_tag="ISB30_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAP1992082.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4370..5527)
                     /gene="mdtE"
                     /locus_tag="ISB30_004525"
     CDS             complement(4370..5527)
                     /gene="mdtE"
                     /locus_tag="ISB30_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HAP1992083.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5587..5916
                     /locus_tag="ISB30_004526"
     CDS             5587..5916
                     /locus_tag="ISB30_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992084.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASI
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(5866..>6009)
                     /gene="gadE"
                     /locus_tag="ISB30_004527"
     CDS             complement(5866..>6009)
                     /gene="gadE"
                     /locus_tag="ISB30_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAP1992085.1"
                     /translation="KNKNESNITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGIT
                     SYF"
BASE COUNT         1470 a         1498 c         1509 g         1532 t
ORIGIN      
        1 aactgttatg caaaactact ttgtggataa aatttggtcc taccaaatat ggcagttttt
       61 gcacacagaa acagtctggc gtcatttcat tagtatactg acattgaaat aatcgcagta
      121 atgaaatata agggatagtc atggctcatg tctgctcggt gatcctcgtt cgtcgttcat
      181 tcgatattca tcatgaacag caaaaaatat cgttgcataa cgagagtatc ctgctgctgg
      241 ataaaaattt ggcagacgat tttgcgtttt gttcactgga tacgcgacgg ctggatatcg
      301 aagagctgac agtttgccat tacttacaaa atattcgtca gttgccacgc aatttaggat
      361 tgcatagcaa agaccgtttg ttaattaacc agtcaccccc catacagctg gtgacggcga
      421 tttttgatag tttcaatgac ccccgggtca attcgccgat actgagcaaa atgctctatc
      481 tttcctgttt atcaatgttt tctcataaga aagaactgat ccccttactt ttcaatagta
      541 tcagtactgt ttcaggaaaa gttgaacgcc ttattagctt tgatatcgct aaacgttggt
      601 atctacgcga tatcgcagaa agaatgtaca ccagcgagag tctcatcaaa aaaaagttgc
      661 aggatgaaaa tacctgtttc agtaaaatat tactcgcctc caggatgtcg atggccagac
      721 gattactcga gttacgtcaa atacctctgc atactattgc ggaaaaatgt ggctatagca
      781 gtacgtcata ctttataaat acatttagac aatattatgg tgtaacgcca catcagtttt
      841 cgcaacattc gccgggtacc ttttcctgac atatttcgca tttgaatatt ggtcaggatc
      901 tcacgtctgc ttcatgtgaa actctttcct gatgatttct gccgggctac cggctagttc
      961 tctttcgcag tttgccaggc ggtataggta ttttcaggag gaaaagactc ggcatgtttg
     1021 ggattattaa gctaacaatt catacgatta cgggaatgtg ggtcagtatt gtgctgttca
     1081 aattgatgac gaatggctgg agtggtttct actttcagtg ctgtgtacta agcctggtat
     1141 tcctgacggt tagctggttg ttgagtggtg aatggttagc aggaaagagt aaggctgaac
     1201 cctcacgttc aaccttactc tcatttacac gttacgcttt tttaaagcgg gcaaagagat
     1261 gttccactac aacgaaaaag accggaacga agtaaattgc cagcactgtt gcggcaaaca
     1321 tcccgcccat tacgccggta cctaccgcgt tttgcgcgcc agagccggca ccatgactga
     1381 taaccagcgg cagcacgccg agaataaagg ccagagaagt catcaggatt gggcgtaaac
     1441 gcatacgcgc tgcttcgatg attgcctcta ccggtgtttt cccttctttc tgcatcatct
     1501 caacggcaaa ttcgacaatc aggatggcgt tcttggcgga aagcccgatg gtggtcagca
     1561 aaccaacctg gaagtaaacg tcattactta agccgcgcag atcggtggcc agtaatgcgc
     1621 caacgacgcc taacggaaca accaacatca ccgagaacgg aattgaccag ctttcataaa
     1681 gtgcggcgag ggcgaggaac accacgacca gtgaaatggc atatagcgcg ggagcctgat
     1741 ttgaggataa cgcttcctga tacgacagtc cggtccatga gtagccgacg cctgccggaa
     1801 gttttgcgac caggtctgcc ataaatttca tggcgtcacc ggtacttttc ccggcagccg
     1861 cttcacctaa aatctccatt gacgggatgc cgttgtagcg ttccagtcgc ggtgaaccat
     1921 aggtccattc ggtagacgaa taggcagaaa gcggtgccat cgtgccagag gcgttgcgta
     1981 cataccattg gttgatgtta tccggcaaca tacggaacgg cgtgcctgcc tggacataca
     2041 cttttttcac ccgcccctgg ttgaggaagt cgttcacgta gctgctgccg aaggcggtgg
     2101 aaattgtctg gttgatatca gacagcgcca cacccatagc ctcggctttc gcagcgttga
     2161 cgttcacttt gaacatcggc gtatcttcca ggccgttcgg gcgtaccccg atgacctgat
     2221 tcggtgattg cgctgccagt gataacagct cgtttcgcgc ctgggtcagt ttttcgtgcc
     2281 ccagattgcc gttgtccagc agttccatat caaaacctga cgcggtaccc agttcagcca
     2341 ccgcgggtaa gttgaacggg aagacgacgg ctttattgat actgcttagc gcaatcattg
     2401 cccgctgaat aatcgcggta accgagtttt cctcgccgac acgttcagac cacggcttga
     2461 ggctgataaa cgccagaccg ttgttttgcc cctgaccact gaagccaaag ccaccaacgg
     2521 taaacaccga ctgaacatta tttttctctt ttgtcagata ataatccgtc acctgttgta
     2581 gcactttcgt ggtgttaacc atggttgcac cggaaggtaa ctgcgcggtg gtcataaata
     2641 ccccctgatc ctcttctggt aagaaagagg tcggcgtacg caggaacagc accgccatcc
     2701 cggcgcaaat cagtaggtag accaccatgt agcgaccggt acaacgcaac agcgagcggg
     2761 tgctgtcggt atagtgctga gttgattttt caaacagcgt gttgaagcgt gcgaacaggg
     2821 cgttaggttt gtgaccgccc tccggcgcgg ctttcagaat ggtggcgcac agggcagggg
     2881 tcaggctcat tgctacaaat actgaaagca gcatggagga gatcagcgtg atggagaact
     2941 ggcggtagat ctccccggtc gcaccgctca taaaggccat cggcataaac actgcggaaa
     3001 gaacaacggc aatcccgacc agcgcacgtt ggatctgccc catcgattta tgcgtcgctt
     3061 ccttcggcgg tagcttatct tccgcaatga cacgctcgac gttctccacc accacgatgg
     3121 cgtcatccac cagtaaccct atcgccagca ccatcccgaa catagttaac gtgttgatgg
     3181 tgaaaccaac tgccgacaag atcgcaaacg tcccgagaat aaccaccggt acggcaatcg
     3241 tcgggatgat tgtggcgcgg aaattctgca aaaacagata catgaccagg aagacgagga
     3301 tgatagcctc aaccagcgtt ttgaaaactt cctgaataga aattttgata aacggtgtgg
     3361 tgtcgtaagg ataaaccgtc ttcaggcttg cagggaaata agccgataaa cggttcagct
     3421 cctctttcac cgctcgcgag gtatccaggg cgtttgctcc ggtagccagt ttgatggcga
     3481 tcccggcggc aggtttgccg ttatagcgcg ccacggtgga ataatcttcc gccccaagtt
     3541 cgacgcgggc gacatcacgc agcagcactt gcgaaccatc ttgctgaact ttcaacagga
     3601 ttttgccaaa ttcttccggc gtttgcagac gcgtctgcac aatgatcgag gcgttcagtt
     3661 gctggtctgc cgcctgtggc atgccaccca gttgaccacc agaaatctgg ttgttttgca
     3721 ccttaatctg ggaaataaca tcggaaggta ccaggttata tttattgagt ttttgcgggt
     3781 ccagccagat acgcatggcg tactcggaac caaagagctg tacgctacca acgcccgcgg
     3841 tacggcttag cgggtctttg atattagacg ctacatagtc cgcgatatcg tactggttga
     3901 ggctgccgtt atcagaaata aatgccgcta ccatcaggat attactgctc gacttatcga
     3961 cgctaatccc ctgctgctgc accgcttcag gtaatgaagg catagcgagt tgcagtttat
     4021 tttgcacctg aacctgtgcg atatcaggcg atgtccccgt ctcgaaggtc agagtgatgg
     4081 aggcattacc tgccgcatcg ctggttgaag acatgtacat caggccatca agcccattca
     4141 tattttgctc aatcacctga gtgaccgagt cttctaccgt ttgcgcatcg gcacccggat
     4201 aggttgcgct gacggtaatg gtcggtggcg caatctgcgg atactgcgca accggtaagt
     4261 tcatgatcgc cagaccgcct gcaagcatca taataatggc aagtacccag gcaaaaaccg
     4321 ggcgatcaat aaaatagtta gccatgaaag tccccttaag cctgcaacgt tattgtttcg
     4381 attcggtgct ggcgttttcc tggctggagg aaattgctcg tgctttgata cccggacgaa
     4441 tgcgttgcaa gccggaaacg atcacccgat cgccagcctg caagccagag gtgacaaccc
     4501 actggtcgcc gatggctttg ctggcttcga tttcgcgtag ctgcacgaca tcgtctttat
     4561 ccagaatgag tgccgttgct ttaccctggg cgttgtgggt gacgccttcc tgcggcacca
     4621 gtaatacatt ctggcggcta ccttcatcca ctaatgccgt aacatacatg ccaggcagca
     4681 agtcaccatt tgggttgggg aaaatcgccc gtaacgtcac ggagcccgtg gtttcatcca
     4741 ccgtcgggtc ggagaatttc agcgtgccgg tctggccgta acgtttacca ttttccagat
     4801 tgagctgtac tggcgtactg ccctgaacct gtttgatttg cccactggcg acctcttctt
     4861 tcatgcgcaa gaaatcctgc accgactgcg tgagatcgac ataaatcggg tccagacgtt
     4921 gtacggtaac cagcgaatct gcctgattag cggtaacgag tgcgccgacg gtcaccgacg
     4981 atttcccgct gacgcccgta atcggcgagg tgacattcgc gtattgcaga ttgatcgtcg
     5041 cctgttcaac agccgctttg gcgacggtga cattggcttc tgcttcattc aactgggtgc
     5101 gcgcggtgtc gtaatcctga cggctaacat agttggtctt cagcaacgat gcctggcggt
     5161 taaaggtgat gcgggcattg ctggcggtag agagcgcttt cgccagcgag cctttggcgg
     5221 agttcagctc tgcctgtaaa ggcgcaggat caatctgata cagcgaatcg ccctggttca
     5281 ctttatcgcc ttcgataaag ttgcgtttaa tgataattcc gcccacctgg ggacgtatct
     5341 cggcaacttc ataaggaacg gttctaccgg gcaattcgct caacacattg accgaaccgg
     5401 gggagagtgt gacgacaccg acctcaggcg tcatggcggc ggcgttttcc gccgatttgt
     5461 catcgcaggc ggtgagcatc gcgccgcaga ataacaacgg tattaacagt tttcttcttc
     5521 tgttcatttt agtccctgaa aattcttgag aaataacggt tctttaaatt gcgcgcgagg
     5581 agagatatga gggatttaca gaccagcggc atagtggggt tgtcagccag taagatggga
     5641 tgcaagcaca gtgatcgaca tggtgaggtc aacgacaaaa aaaatgttgc cgttctgcca
     5701 acagtttctg ccagcattcg ggcaacgaag ggttctactg gtggatacac ataccagggg
     5761 aataaagtcg ttttcgtctc tccttcaata atcaccgggg gtattgaaat cagcatctgc
     5821 atccctcgtc atgccagcca tcaatttcag ttgcttatgt ccttactaaa aataagatgt
     5881 gatacccagg gtgacgatgt cgctcatacg tcttaatttc agcttgtaca taatgttgaa
     5941 tttctgaatc tttaatgttt gttgcgaaat gttgagcgtt gacgtgatat tgctttcatt
     6001 tttgttttt
//
LOCUS       DACADF010000223         5915 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.225, whole genome shotgun sequence.
ACCESSION   DACADF010000223 DACADF010000000
VERSION     DACADF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.225"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            172..753
                     /gene="mdaB"
                     /locus_tag="ISB30_004528"
     CDS             172..753
                     /gene="mdaB"
                     /locus_tag="ISB30_004528"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAP1992086.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            784..1098
                     /locus_tag="ISB30_004529"
     CDS             784..1098
                     /locus_tag="ISB30_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP1992087.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(1143..2030)
                     /locus_tag="ISB30_004530"
     CDS             complement(1143..2030)
                     /locus_tag="ISB30_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP1992088.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(2027..2974)
                     /locus_tag="ISB30_004531"
     CDS             complement(2027..2974)
                     /locus_tag="ISB30_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP1992089.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(2985..4022)
                     /locus_tag="ISB30_004532"
     CDS             complement(2985..4022)
                     /locus_tag="ISB30_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP1992090.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(4031..5014)
                     /locus_tag="ISB30_004533"
     CDS             complement(4031..5014)
                     /locus_tag="ISB30_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP1992091.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(5011..5820)
                     /locus_tag="ISB30_004534"
     CDS             complement(5011..5820)
                     /locus_tag="ISB30_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP1992092.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         1672 a         1601 c         1371 g         1271 t
ORIGIN      
        1 aagttgattt aagtattgtc attttcagaa aatatttatc ccgcatcggt gaatcagaga
       61 aagatcagac ttttgctaaa ttcgcaaaag actttgcaca ttttgctaat ttcaccgtac
      121 cgctctgtga cgtactatag tcggcaaacg tctcaccttg aggttaaaaa aatgagcaac
      181 atcctgatta tcaacggcgc gaaaaaattc gcccactcca atggtcaact gaacgacacc
      241 ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc atgatgtccg cattgttcgc
      301 gccgacagcg actacgatgt caaagcggaa gtacaaaact ttctctgggc tgatgtggtg
      361 atctggcaga tgcctggctg gtggatgggc gcgccgtgga cagtgaaaaa atatattgat
      421 gatgtattca ccgaaggtca cgggactctg tatgccagcg atggtcgtac ccgcaaagat
      481 ccatcaaaaa aatacggttc cggcggcctg gtacagggca aaaaatatat gctttctctg
      541 acctggaacg caccaatgga agccttcacc gaaaaagatc agttcttcca cggcgttggt
      601 gttgacggtg tgtacctgcc gttccataaa gcaaaccaat tccttggcat ggaaccgctg
      661 ccgacattta tcgctaatga cgtgataaaa atgcctgatg ttccccgcta tactgaagaa
      721 tatcgcaagc atcttgtgga aatttttggt taactagagc tctggcttta gaaggagtta
      781 accatgatta cagtaatcgc agaaattcgt actcgtcctg gtcaacatca ccgtcaggca
      841 gtgttggatc agtttgctaa aatcgttcca accgtactga aagaagaagg ttgccacggc
      901 tatgcgccaa tggtggattg cgctgctggc gtgagtttcc agtctatggc accggattct
      961 atcgtgatga ttgagcagtg ggaaagcatc gcgcatcttg aagcgcatct gcaaaccccg
     1021 cacatgaaag catatagcga agccgtaaaa ggtgacgtgc tggagatgaa tattcgtatc
     1081 ctgcagccgg ggctttgaca tttctaaaaa gaggccggtg ctgctaccgg tctcagccca
     1141 gattagccat tctttcttcc cgttttcagt cctcgatgga aatcgttaat tcgcttaatg
     1201 gttttaaccg cacgctgcgg cactgcggaa gctaccgacc agacaatcat ttccagacac
     1261 aacaataccg taccatgtag cggcgttttc cctttttcat ccccgcgcgg aacatgaatc
     1321 acgatactgg cgtctttgct aaaacgtgaa tccagcgcgt ttgtcagcaa aataacaggg
     1381 atccccagcc gcctcgcctc acgaagcgtt gtcagccctt cccggtgcgc agatttttgc
     1441 gccatcatga tcagaacatc gccacgttga agcgcgatca gttgctcggc aaggccaata
     1501 cccgtacggt ttagtgccgt ggcaggcaaa cctatgcggt tgaatagtcg tgcggtatag
     1561 tccgccagaa tacccgaagc gccaatgcca aaaattgcga cttgccgtgc ctgcaccaac
     1621 aacgctacag cttgcgcaag cgcgtggcgg ttttcaggct ctgacaggac attacaggta
     1681 tacaaatgcc cttcaagaac aaagtcgatt gcggtgttaa catcgctcgt caagttactc
     1741 accgtcgtag acatcttttc gctggaacta agggctggac caagccactg ttccagagtg
     1801 cgtttcagat cacgcagccc ggcaaacccc agggcctgta tagccctgat aactgtggca
     1861 tcggaagtgt tcaacgtggc cgcaatttcc atcgccgttt gctcgagtac cacttcacga
     1921 tgctcattaa tgtagctggc gacaactctt aacccaggcg ttaactgact cgcgcgagtg
     1981 cgaaaacgct caccaaaaat atcaacccgc actttatctt tctggatcat ttagcctccg
     2041 gtagaggtcg ccctgcaatt tgtgattgta tctgcgcagc catcagcccc agagatgcaa
     2101 aggagttaga aattgcctct tcacgagaaa tcagcacata acgcccggaa cgacaggcag
     2161 tcagaaactg acaccatccc ggcatgactt tttccattgc tgccagctca tcctgaggtt
     2221 taccaccggt gtcaccgcgc caggttgcaa aaacaaaatc ggcatccagt tcaggtaaac
     2281 gctccgcgct cacatccatt cgcccacctt caggaatgct ctcaatcagc ggcggaaaac
     2341 gaaaaccagc atcgcgtaat acccgtccca gcgaatgata gctatgcatc acattaattt
     2401 ttccctggtt tgcctgaatc accgataccg ttattttttg gctgtccagc gtcgccttca
     2461 gcgcattgat ttgtgcctga tagcggcgtt caagtatcgc cagctgcgac tgcgttcctg
     2521 taagttctgc cagcttgcga tagatttctg gcgcgccccc tttgagatga tcaatgctca
     2581 ccgtgggcgc aattttttca agttgttcaa tgggcgtatt acgggtcggt tctgtgatga
     2641 tcaagtcagg ctttgccgcc acaatcgctt caatatcaat atcagcggta ccaataaagg
     2701 cgatcgatga attatcaaag tcgacacctg tcaataacgc gccagatcga atgaaatggc
     2761 taccgtcagg ccgagttcgc ccgtggctgg cgacaggagg tacaccgagc tcaatcagag
     2821 gaatagtgat atcaagatcg tgtaaagaga caattctctt tggatgagca ggaacagtga
     2881 ctttgcgccc taaatcatca gtaaatacct gaacaggttc tgttgcacgg gaaaaaaatg
     2941 acaacagtat cagcagagaa aaaaataaac gcatataact cccgttaaaa actgttcctg
     3001 cgctgccaga gcagtagtaa aaaaaatggt ccaccaatca gtgagataat gattccggca
     3061 ggcagttgca aaggcaaaaa agccaaacga ccaatgctat ccgccagaat aactaacaac
     3121 gcccccgata ccgcgctccc cagcagtaat gtcgtctgcc cgccacgtag aataaaacgt
     3181 gatatatggg gtgctatcaa tccgacaaaa ccgatgttac caacacacga aacgcagacg
     3241 gctgtcagga ttatgggtgc ggcgacacgt aacaacgcca gacgagaact attcacccct
     3301 aacccggttg caacctgatg accaagcaga gcgatatccg cagtcctggc ggtgaacagc
     3361 aataaaagag cggctggcaa catccaacag gctgaaatac cgactaacat ccagttagct
     3421 gcatgcaggc ttcccgataa ccacatcaat gcagtttgta catctcgaac atctgcagtg
     3481 gtcataaata caccgatacc ggcggcgaaa aaccaggaaa caccaatacc aatcagaaca
     3541 aatcgcgggc gggaaatatc gcgcgcacaa aaaatcacga ttaaagccac caacaggcca
     3601 ccagcaagac cagccactgg acgccagaac attcccagca ttggaaactg gaaaatcagc
     3661 cacagcacag ccacgctaca tccttctttg acgccgatta aaccaggatc ggcaaggccg
     3721 ttacgtgcaa tggattgcat tgcggcccct gccataccta acatggcccc gcatagcaat
     3781 gccatgatga ctctcggcaa gcgaatatcc tgcacaatat aacgtgcgtc tgtgcttacg
     3841 ttttctggag caaacaacgc tcgtcctact tccgatgccg gaacagagaa agaaccctgc
     3901 atcagaccga atatcagtat tccaaccgca agcaggaaca gcccaccaag caacagcaga
     3961 tttttagggc gcaccagtgt cgaaaactta ccaacacgta gtgggcgtag cccgacgttc
     4021 attatccgac tcatttgaac atcctcgtcg ccataatgac aaaaacaggc gcaccaacta
     4081 acgccgtcat gacaccggtc gctaactcat ggggcgtgaa aagcgtccgg gcaataatat
     4141 ctgccagtaa taacaaaagc gcgccgacac aagctgaaag agggagcaga acgcgcagat
     4201 ccgctgatac cagacgccgg acaatctgcg gtactagcag gccgacaaaa ccaatggggc
     4261 cagcaatcga tacggctgcg ccacagagca acgcgatcgc aagcagggtg aatgttcgcg
     4321 tgcggagtac agagacgccc agcccctgcg ccatgcgatc gcccagtgcc aacatgttaa
     4381 gggagggtgc aaggtaaagg gctaacacaa atccccccag agaaaaccag gcggacgtac
     4441 ccagcgtcgc ccaatcctgc cccgctaaat cacctgctag ccatgtccgc atcgccagca
     4501 gtgtttgttc gtcaagaatc aggattgcag ccgttattga ggacacgaac gccgatagcg
     4561 ccacgccgca taaagtaact ttcattggcg ttaaccctga acgccctgcg gaggaaagca
     4621 gcaatatcaa caggaacaat aacgcgcccc ccgtagatgc cagcagtggg cgtccaaccg
     4681 gaaaggccag tccaagcgca ctggcggcaa cgacagcaag agcggctccg gcatttaagc
     4741 ctaaaatatg tggttccccc aatgggttac gaatgacagc ctgcaacaaa gcaccagcaa
     4801 caccaagaga agcaccggta agcaatgctg ccgctaaacg cggcaaacgg agtcttacaa
     4861 ttacgttgtg atcgaagttt tgcggatcaa aatgaagaaa tgcctgcaac accgtttgtg
     4921 gtgcaataaa tcgggcacca atccctggat ataccacgca tcccaccagc agtaaggcga
     4981 ggaaaattac agtaaccagc cctgtgcgca tcatactttt tctacaccac gaaacggcat
     5041 gaagaacggc tttcccgtca gaggatttat tgatgcgtga acatcaacgt caaagacagc
     5101 ttttaccagt tcaggcgtgc aatgctcccc ctcattcaac acacgcacca ctttcccctg
     5161 gcgtaaaaat atcaacgtat cgccataatt gacggcaaaa ttcagatcat gcaacaccac
     5221 gacgactgta cgcccgtgat gacgcgttaa atcatgtaat aactcaagaa tttcaacctg
     5281 ataacgtaaa tcaagccagg ttgtgggttc atccagcagg atataaggcg tcttttgcgc
     5341 caacaccatc gcaatccagc aacgctggcg ctgcccacca gagagttttt caacaggtaa
     5401 atgtgcaaac tcctgcgtac cggttaattt cagggcctct tccaccgcct gctcatcggc
     5461 gtcgctccat tgacgtatga aattttgcca gggaaaacgc ccacgcgaca ccagttcata
     5521 aacggttaag ccctccggca ataatggcga ctgcggcaag atcccaagtc tacgggataa
     5581 cgctttcgtt ggttgctcgt gaatcgcttt gccatcaagc aatatcgaac cgcccatagg
     5641 ttgcaagata cgtgcaatgg tgctgagcaa cgtcgacttt ccacagccgt ttgccccaac
     5701 cagaacggtc attttttctg tgggaacgga gaaagaaaca ccatcaacaa tgattttctt
     5761 gtgatacccg gcagaaacat tgtcgaggat cagcccctgc ccttttgcgt tatgtgccac
     5821 gtgtacgcca acttaatcaa agacaaacaa aaataaaact cattctcctt tgagagtttg
     5881 acgccacaaa atgtagtagt caagcagtta aaagc
//
LOCUS       DACADF010000224         5915 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.226, whole genome shotgun sequence.
ACCESSION   DACADF010000224 DACADF010000000
VERSION     DACADF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.226"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            223..1389
                     /gene="nhaA"
                     /locus_tag="ISB30_004535"
     CDS             223..1389
                     /gene="nhaA"
                     /locus_tag="ISB30_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAP1992093.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLEGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1455..2354
                     /gene="nhaR"
                     /locus_tag="ISB30_004536"
     CDS             1455..2354
                     /gene="nhaR"
                     /locus_tag="ISB30_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAP1992094.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSSLFSPASR"
     gene            complement(2412..4451)
                     /locus_tag="ISB30_004537"
     CDS             complement(2412..4451)
                     /locus_tag="ISB30_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HAP1992095.1"
                     /translation="MSFIKQDPHRLVWQQDDRYLWIEAWGENSLRVRSGRHLPMMRNE
                     NWALTEEKGDAVAYITYEEKQATLQNGKITAIVNLQGQISFWRNAEKCLLQEFWRQRG
                     EIGEDESAHGQYVSALNLQAREFKPIPGGKYTIKARFEANDGEKLFGMGQYQQPNLDL
                     KGCMLELAQRNSQASVPFLLSNRGYGFLWNNPAVGRVTFAQNGTEWVAEVSEQLDYWV
                     TAGDTPAEISAAYARVTGTPPMMPDYAMGFWQCKLRYRNQQELLEVARGYKQRNLPIS
                     VIVIDFFHWPNQGDWMFDLRDWPDPDAMIAELKEMGIELMVSFWPTVDNRTESYREMK
                     ENGWLVHTERGLPINMDFLGNTTFFDATHPGAREYVWNKAKRNYYDKGVKLFWLDEAE
                     PEFGVYDYDNYRYYAGPVLEVGNIYPRMYAKTFFDGMTAAGEKQVINLLRCAWAGSQK
                     YGALVWSGDIHSSFRSLRNQFAAGLNMGIAGIPWWTTDIGGFHGGNIHDPKFHELLIR
                     WFQWGVFSPVMRLHGNRDPQVLPEQPYRDGIAQCPTGAPNEVWSYGEETGEILTSCLQ
                     LREKLKSYISKIMAETHEKNSPIMRTMFFEFPDQAESWNIYDQYCFGPDLLVAPVMHE
                     GTRSRDVWLPAGETWIDFYTHEHYAGGQTLHHCAPLHQIPVFIREKGKYRQIFPV"
     gene            complement(4479..5852)
                     /locus_tag="ISB30_004538"
     CDS             complement(4479..5852)
                     /locus_tag="ISB30_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1992096.1"
                     /translation="MSSVLETDKSSASDSLPLIQRISYGSLDVAGNLLYCFGSTYILY
                     FYTDVAGISLAVAGIILLLARIVDGIDAPVWGIIIDKTHSRYGKCRPWFLWLPLPFAV
                     FSALSFWSPDISMTGKTIYSAISYMLASILFTGLNTPLSAILPLMTLSPKERLVLNSY
                     RMTGGQIGVLLMNATALPLVAFLGNGNDKAGFIYTAIVFSVISCALTLFAFKNIRELD
                     TDKIQQEPRLPMKKSFSAMKGNWPWILMVLANLIFWIALQQRNTTIVYYLTYNLDRKD
                     LVPLINSLATIQILFIIAIPFFSRYLSKTWIWITGLLVAMFGGGLMWLAGDNIPLMIA
                     AWMLANIGSGIACSMPFAMLGFAVDFGRWKTGIKATGILIAFGSTFCIKMGSGIGTAF
                     AAWIMNSFGYIPNQPQTAAGLDGISWAFIWVPAVLFALAAIPLLFFRKYEAMEECIQH
                     DLQVHNP"
BASE COUNT         1485 a         1413 c         1466 g         1551 t
ORIGIN      
        1 cgaataattt cttcagaaat cttgacatta gactatgatt aatggggttt aaggtcatca
       61 gccgccgctt tcgggcatca aaaactcatc tctcgctgat ggcgcaattc ttttaataga
      121 tcgtaaaaag cgagctattc ctacactata atctgatttt aacgatgatt cgtgcggggt
      181 aaaatcgtga aaacgatcta ttcacctgaa gagaaataaa aagtgaaaca tctgcatcga
      241 ttctttagca gtgatgcctc gggaggcatt attctcatta ttgccgctgt attagcgatg
      301 attatggcca acagcggtgc aaccagtgga tggtatcacg actttcttga gacgccggtt
      361 cagctccggg ttgggacact tgagatcaac aagaacatgc tgctatggat caatgacgct
      421 ctgatggcgg tatttttcct gttggttggt ctggaagtta aacgcgagct gatgcaaggt
      481 tcgctggcca gtctgcgcca ggcggcattt cctgttattg ccgcaatcgg cgggatgatt
      541 gtcccggcat tgctctatct ggcttttaac tatgccgatc ctatcactcg cgaaggctgg
      601 gcaatcccgg cggcgactga cattgccttt gcacttggtg tgttggcgct gttgggaagt
      661 cgtgttccgt tagcgctgaa gatctttttg atggctctgg ctattatcga cgatcttggg
      721 gccatcatta tcatcgcatt gttctacact aatgacttat cgatggcctc tcttggcgtc
      781 gcggctgtag caattgcggt actcgcggta ttgaatctgt gtggtgtacg ccgcacgggc
      841 gtttatattc tggttggcgt ggtgctgtgg acagcggtgt tgaaatcagg tgtccatgca
      901 acgctggctg gcgtcattgt cggcttcttt attccgttaa aagagaagca tgggcgctct
      961 ccggctaaac gtctggagca tgttttgcat ccatgggtgg cgtatctgat tttgccgctg
     1021 tttgcatttg ctaatgctgg cgtttcactg caaggtgtca cgctggaagg tttgacctcc
     1081 attctgccat tagggatcat cgctggtttg ctgattggca agccactggg tattagtctg
     1141 ttctgctggt tggcgctgcg tttgaaactg gcacatctgc cagagggaac gacttaccag
     1201 caaattatgg cggttggtat cctgtgcggt atcggtttta ctatgtctat ctttattgcc
     1261 agcctggcat ttggtagcgt agatccagaa ctgattaact gggcaaaatt aggtatcctt
     1321 gtcggttcaa tttcttcggc ggtaattgga tatagctggt tacgcgttcg tttacgtcca
     1381 tcagtttgac aggacgatgt actggggagc cataaacggc tcccttttca ttgttatcag
     1441 ggagagaaat gagcatgtct catatcaatt acaaccactt gtattacttc tggcatgtct
     1501 acaaagaagg ttctgtggtt ggcgcagcgg aggcgcttta tttaacacca caaaccatta
     1561 ccgggcagat ccgggcgctg gaagagcgcc tgcaagggaa actatttaag cgtaaaggac
     1621 gtggtctgga acccagcgaa ctgggggaac tggtctatcg ctatgccgat aaaatgttca
     1681 ccttaagcca ggaaatgctg gatatcgtca actatcgcaa agagtccaac ttattgtttg
     1741 atgttggtgt ggcagatgca ctttccaaac gtctggtcag tagcgtactt aacgccgcag
     1801 tggtagaagg cgagccgatt catcttcgct gcttcgaatc cacccacgaa atgctgctgg
     1861 agcaattaag tcagcataaa ctggatatga tcatttccga ctgtccgata gattctacgc
     1921 agcaggaagg cctgttctcc gtgagaattg gcgaatgtgg cgtgagtttc tggtgtacaa
     1981 atccaccacc ggaaaaaccg ttcccggctt gtctggaaga acggcggctt ttgattcctg
     2041 ggcgacgttc aatgttaggg cgcaaattgc ttaactggtt taactcccag ggattaaacg
     2101 tagaaatcct tggcgagttt gatgatgccg cgttgatgaa agcttttggt gcgatgcaca
     2161 atgcaatctt cgttgctcca acgctttatg catatgactt ttatgccgat aaaactgtcg
     2221 tagaaattgg tcgcgtcgag aatgtgatgg aagagtacca tgctattttt gctgagcgga
     2281 tgattcagca cccggcggta cagcgaatct gcaatacgga ttattcttcg ttgttttctc
     2341 ccgcctcacg atagctatta aaactcacgc cccgctacat acggggcgtg ctaatgaaca
     2401 aacgatgtcc tttaaacggg aaatatctgg cgatatttcc ctttctcgcg aatgaatacc
     2461 ggtatctggt gcagcggtgc gcaatgatga agcgtttgcc cacccgcgta gtgttcatgg
     2521 gtataaaaat cgatccatgt ctctccagca ggcaaccaga catcacgtga acgtgtacct
     2581 tcgtgcatta caggggcaac gagaaggtct gggccaaagc agtactgatc ataaatattc
     2641 cagctttctg cctggtcagg aaattcaaag aacatcgtgc gcattatcgg gctgttcttc
     2701 tcgtgcgtct cagccataat tttactgata taagatttca gtttttcacg caactgcagg
     2761 caggaagtga gaatttcacc ggtttcttca ccatagctcc agacttcatt cggcgcgcca
     2821 gtcggacatt gtgctatgcc atcacgatag ggttgttcag gcaaaacctg cgggtcgcgg
     2881 ttgccatgta aacgcattac tgggctgaat accccccact ggaaccagcg aattaacagt
     2941 tcatggaatt tcggatcgtg aatattaccg ccgtggaaac caccaatatc ggttgtccac
     3001 cacgggatcc cggcaattcc catgttcaag ccagcggcaa actgattacg caaagaacgg
     3061 aaagaggagt gaatatcgcc tgaccacacc agagcgccat atttctgact gccagcccag
     3121 gcacagcgca gcaggttaat cacctgcttt tcaccagccg cggtcatacc atcgaagaac
     3181 gttttggcgt acatacgcgg atagatatta ccgacttcca gcacaggccc tgcgtagtaa
     3241 cgatagttgt cataatcgta aacaccgaac tctggctcgg cttcatccag ccagaacagt
     3301 ttcacgcctt tatcgtaata attgcgtttg gctttgttcc agacatattc ccgtgcgcca
     3361 ggatgggtag catcaaagaa cgtggtgttg ccaaggaaat ccatattgat aggcaaacca
     3421 cgctcggtgt ggaccagcca gccgttttct ttcatctccc gataactctc ggtacggtta
     3481 tcaaccgtgg gccagaagga gaccatcagt tcaatgccca tctcttttag ttcggcaatc
     3541 atggcatcgg gatcgggcca gtcgcgcaga tcgaacatcc agtcaccctg attcggccaa
     3601 tggaagaaat cgatgacaat caccgaaata ggcaaattac gctgcttgta gccacgtgca
     3661 acttctagta actcttgctg attacggtag cgcaacttgc actgccagaa tcccatggcg
     3721 taatcgggca tcattggcgg cgtaccggta acacgtgcat aggcggcgct gatttctgcg
     3781 ggagtatccc ctgcggtaac ccagtagtcc aactgttcgc taacttctgc cacccattcc
     3841 gtgccatttt gcgcgaaagt gacgcgaccc accgctgggt tattccacag aaaaccatag
     3901 ccgcgattag aaagcaggaa gggaaccgat gcctgagaat tacgctgcgc cagctctagc
     3961 atacagcctt ttaaatccag atttggctgc tggtactgcc ccatgccaaa caacttttcg
     4021 ccatcgttgg cttcgaaccg cgccttgatt gtgtatttcc cgccgggaat cggtttgaac
     4081 tcgcgggcct gtaaattcag ggcgctgaca tactgaccgt gggccgactc atcttcgcca
     4141 atttcgccgc gctgacgcca gaactcctgc agcaggcatt tttctgcatt acgccagaaa
     4201 gagatctgac cctgcagatt aacaatggcg gtgattttgc cgttttgtag tgttgcctgc
     4261 ttttcttcat atgtgatata ggcgacagca tcaccttttt cttctgttaa tgcccagttt
     4321 tcattgcgca tcatcggcag atggcgaccg ctacgcacac gtaaactgtt ctcaccccat
     4381 gcttcaatcc agagataacg atcgtcttgt tgccagacca gacgatgggg atcttgctta
     4441 ataaacgaca taatttcctc caggagcttg tgacatgttt aagggttatg aacttgcaaa
     4501 tcgtgttgga tgcactcttc catcgcttca tatttacgaa agaagagcag tggaatggcg
     4561 gcgagtgcaa agagcacggc tggcacccag ataaacgccc agctaatacc gtccagaccg
     4621 gcggcagttt gtggctggtt gggaatatag ccaaagctgt tcataatcca ggcggcgaag
     4681 gcggtgccga taccactgcc catcttgatg cagaaggtac tgccgaaggc gattagaatg
     4741 ccggtagctt taatccctgt tttccagcga ccaaaatcaa ccgcgaaacc aagcatggcg
     4801 aagggcatag agcaggcaat gccactgcca atattcgcca gcatccaggc ggcaatcatc
     4861 aaaggaatat tgtctccagc cagccacatc agtcctccgc cgaacatggc gaccagcagg
     4921 ccggtgatcc aaatccaggt tttggagagg taacggctga aaaacggaat tgcgatgata
     4981 aacaaaatct gaatggtggc gaggctgttg atcaagggaa ctaaatcttt gcgatccagg
     5041 ttgtaggtta aatagtaaac aatggttgtg ttgcgctgct gtaaggcaat ccagaagatg
     5101 agatttgcca gcaccatcag gatccacggc cagttgcctt tcattgccga aaagctcttt
     5161 ttcatcggca gacgtggttc ctgctgtatt ttgtcggtat ccagttctcg aatatttttg
     5221 aaggcaaata gcgttagcgc acaggagatt acggaaaaga cgatagcagt ataaataaat
     5281 cctgccttat cattaccgtt tccaagaaaa gccactaacg gtagcgccgt cgcattcatt
     5341 aataacacgc caatttgccc gccggtcatc cggtaagagt ttaataccaa ccgttctttt
     5401 ggtgataacg tcatcaaagg taatattgcg cttagcggcg tgtttaaacc ggtaaaaaga
     5461 atgctggcta acatgtacga tattgctgaa tatatggttt tacctgtcat actgatatct
     5521 ggcgaccaga atgagagtgc gctaaataca gcaaaaggta atggcaacca taaaaaccac
     5581 ggccgacatt taccgtagcg ggaatgcgtt ttatcaataa taatccccca taccggggca
     5641 tcgataccgt ctacaatacg cgccagtagc aggatgatgc cagctacggc aaggcttatg
     5701 cccgccacgt cagtgtagaa atagagaatg taggtggagc caaaacagta gagcagatta
     5761 cctgcaacat ccagcgaacc atagctgatc cgttgtatta aaggcagtga atcggatgcg
     5821 gatgatttat cagtttcaag tacggaactc attatattct ctccagaaca agaatgcctg
     5881 gctcagaaaa acgactttta ctttctaagc aaata
//
LOCUS       DACADF010000225         5766 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.227, whole genome shotgun sequence.
ACCESSION   DACADF010000225 DACADF010000000
VERSION     DACADF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.227"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(207..2525)
                     /gene="yicI"
                     /locus_tag="ISB30_004539"
     CDS             complement(207..2525)
                     /gene="yicI"
                     /locus_tag="ISB30_004539"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAP1992097.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYVTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPTADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(2535..3917)
                     /locus_tag="ISB30_004540"
     CDS             complement(2535..3917)
                     /locus_tag="ISB30_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HAP1992098.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            4210..4304
                     /locus_tag="ISB30_004541"
     tRNA            4210..4304
                     /locus_tag="ISB30_004541"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4244..4246,aa:Sec,seq:tca)
     gene            4357..5394
                     /locus_tag="ISB30_004542"
     CDS             4357..5394
                     /locus_tag="ISB30_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HAP1992099.1"
                     /translation="MSDKDLTQPPAGEFIMFASGDGRVRVECRFESDTIWLSQAAMAE
                     LYDKDVRTINEHLINIFSEGELVQNSTIRKFRIVRQEGKRQVSREIDHYNLEAILAVG
                     YRVRSPRGTQFRQWATQTLQEYLIKGFVMDDERLKNPPVGSSVVPDYFDEMLERIRDI
                     RASERRVYLRVREIFALAADYQPSLKETTQFFQTIQNKLHFACTGHTAAELIHKRADA
                     SQPHMGLTSYKGEEVRKGDVTVAKNYLTQDEVSELNRVVNMWLDFAEDQARRRQQVFL
                     RDWQDKLDQFLQFNDREVLQGAGKISKKMADEKAQAEYVQFAEQQRRLKEAEGEKDIA
                     GLLQWNKESKK"
BASE COUNT         1558 a         1488 c         1349 g         1371 t
ORIGIN      
        1 caacaatact aaaatctgtc cgtaatccgg acagtgataa aacaaagcaa ggcggttaag
       61 ggatattttt cagcacagat cacattttcg tgatgaataa tcgtgatcat gctcgcaaat
      121 acgatggaag aaatatttat taccagatgt gagattattt accggatacg acacaaaacc
      181 attcgtatcc ggcattcttc aatagattac aacgtaattg tcagcgcatt cccttgagga
      241 gtcaccacca gtccctgctc actttcagcc tgcgaaccgc cttgcagacc atttactttc
      301 acaatattgc gcaagcacag tgtccagttc ttcgcctcgc ccgtaccagt cacagtaatc
      361 gtgttgccag tacgtgctgc ttttaaagta aagatcaccg aaccgtcagc agtgggcact
      421 tcacagacgg cttcatgccc gtcttgcaga ttaaagaggt ggaatgccgt accttcgtgc
      481 cacgcgtaat cgggacgttg atcgttgttg cccagcgcca gtagggtgct atcacgcaca
      541 taaacgggca gactcaggaa gctgtgctgc tgtttatgcc agcgactacc gtcgagttca
      601 tcgttgtgcc acaggtgtgt ccagcgacct tccggcaggt agaactgcac atcgcccgct
      661 tcagtgaaca caggcgcaac catcacgttg tcgcctaaca tgtattgacg gtcaaggtaa
      721 tcacaagccg gatcgtccgg gaactccatc atcatggccc gcatcatcgg cgtaccccgg
      781 gcgtttgcac gtgcagcttc acgatacaga tacggcatca tgcggcattt cagttgcgtg
      841 aagaagcgca ccacatcaca ggactcatca tcgtaggccc acggcacacg ataagatttg
      901 ctgccatgta aacggctatg gctggagagc aaaccaaacg cgcaccagcg tttgtaaacg
      961 tgcgccggag cggtattttc aaagccgccg atatcgtggc tccagaagcc aaaacctgaa
     1021 aggccaatag acaaaccacc gcgcaggctt tccgccattg attcgtagtt agcgtaacag
     1081 tcgccacccc agtgtaccgg aaatttctgc gcaccaacgg aggccgagcg ggcaaacaag
     1141 acggcttctt cctcaccaac ggtgtccttg agcacgttcc acaccagttc gttgtagatg
     1201 tatgcataat ggttatgcat tttctgcgga tcggaaccat caaaccattg aacatcggtt
     1261 ggaatacgtt cgccaaagtc ggtcttaaag caatcaacgc ccatcgcgac cagacctttc
     1321 agtttgtcgg cgtaccattt gcaggcatcc ggattggtaa agtcataaat cgccagacct
     1381 ggctgccatt tatcccactg ccacagcgaa ccgtccgggc gtttgagtaa ataacctttc
     1441 tcttgtaact ctttaaagac gggggatttt tgcccaatat aggggttaat ccagacgcag
     1501 attttcagcc ctttcgcttt caggcggcgg atcatccctt ccgggtccgg aaaagtcagc
     1561 ggatcccact caaaatcgca ccactggaag gctttcatcc agaaacagtc aaagtggaaa
     1621 acatgcagcg gcagattgcg ttccgccata ccatcgataa agctgtttac cgtcgcttcg
     1681 tcgtagttgg tggtaaatga agtggttagc cacagaccga aggaccacgc gggcggcagc
     1741 gccggacggc cagtaaaacg ggtataacga tcaagtaccg ctttaggcgt cgggccgtcg
     1801 ataacaaagt attcgagata ttcactctca acgctgaact gtactttgga gactttctcc
     1861 gatcccactt caaaagagac acattgggga tgattgacca gtaccccata accacggtta
     1921 gtcacataaa acgggatatt tttatacgcc tgttcagtac ttgtgccgcc gtcccggttc
     1981 caggtttcta ccgtctgacc attgcgcacc agggcagtaa agcgctctcc caggccgtaa
     2041 actgtttcgc caacgccaag atccagccgt tcaaacatat aatttcgttg attattcgtg
     2101 tcctgcacgt agccattatt tttcacctga ctaccggtaa tacgttcgcc gttgcgcaga
     2161 aaatccagtg accagaactc acctttgctg acacgcgcac ttaagttgcc acttttaaac
     2221 tcagcgtaac gttctgtgtt ttcgatagtg accttcacgt cctgcaaaat attgagcggg
     2281 taatgaggac cgttattcag cgccccctga aaatgctcaa tccgtacacc gacaatacct
     2341 tcctgtgggg agaaaaagcg cagcgtaaat aaaggcgtat caagctgcca ggtacgttca
     2401 cgcacatcac gaggggcagc atagaccacc atttcattac cctgctgttc aacctcgaac
     2461 acctgaagcg ggtgaatcaa attgaggcca ggttgaatca accagtttcc gtcgctaatt
     2521 ttcattgccg ttccttagtt ctgcacttct tgagaggtga attgttgctg gcaacggcgt
     2581 ttgccctgcg ccagctgttc cataacggtt ttcagattgt gcgtcgtgag cgagtagtag
     2641 cgtttagcga taatcgcgct cagcaagtaa cagatcgccg gaacaatcgt gaatagcgcg
     2701 ataatgatgc taattgtggc gctgttctgc gctttttctg ccgcatcata tccgccataa
     2761 gccagcatcc agccgataag ggcaccaccg aaggccaacc ccagtttgag cacaaacagc
     2821 gtgccagcaa aactgatccc ggtcaggcgt ttaccattac accactcgcc gtagtcgacg
     2881 gtatcggaca tcattaccca ctggataggt gtcaccagtt gatgcaacac accaatcacg
     2941 aagatgaaga caaacatggt gatgctggcc tgcataggaa caaagaacat cgcgaggcta
     3001 atcactgcca gcagggcgtt cgtccaccag aagatagtga ctttacattt ccagtcggtc
     3061 agcggttttg ccagtgcgga accaatcagg ttacccacgc aataagtggt gagaaaagca
     3121 acaaatactt ccggcgtacc caaaatccat gtgacgtaat acatcatcgc cccaccacgt
     3181 acgcacaccg ccaggatatt gaaaatggtc aggaaaccga caatacgcca ctggtcgttt
     3241 tgccagatat cacgtaaatc atcccgcata gacgtcgttg taggtggtgc ttcaacgcgt
     3301 tctttagtgg tgaagaaaca gaatgccagc atcataaatg ccaccacgga aaggaccgcg
     3361 ataccgccct ggaaaccgag tggtttatta tcaccaccaa ttaaattaac cagtggcatc
     3421 atcagaacag tagagagcat gcctcccgcg gtcgccagca caaaacgcca ggattgcagc
     3481 gagatacgct gagtcgggtc attggtgatt acaccaccca atgcgcagta agggatattg
     3541 acgatggtat aaagtaaggt aagtagggtg taagtaattg ctgcataaat catttttccg
     3601 ttcatactga gatctggcgt gctataggct agtacacaga cgatcccgaa tggcagtgca
     3661 ccaaacagta cccacggccg aaatttaccc cagcgagagc gcgttcgatc ggccaacaat
     3721 cccatgcaag gatcggaaat cgcatccagt gcacgagcga ccaaaaacat ggttccgaca
     3781 aatccggcag gaatgccaaa aatatcggta taaaagaaca tcatatataa cattacgtta
     3841 tcgaaaataa tgtggctggc ggcgtctccc atgccataac caattttctc tttaacggac
     3901 aacacttcac tcttcatctg cttttccttg atgctatagg ggtgctgaga ccgataacgc
     3961 attttgtaaa ccatccctgc gagagcgaat attccgatat ctggttatta atttatgatt
     4021 cttgttttat gtgatcgtgg tagcgttaat ttcgctcata tatcattgta aaatatgagt
     4081 tttatatgaa ctataatgct ttcgtgataa tacgctgcgt gtattaggcg gaaaaaactg
     4141 atctggggga tgtagaaact caaggaagta gctataatgc gccccgcctc catgtagcaa
     4201 tcgaggcgcg gaagatcgtc gtctccggtg aggcggctgg acttcaaatc cagttggggc
     4261 cgccagcggt cccgggcagg ttcgactcct gtgatcttcc gccaaaatgc ctctcctgaa
     4321 ctctcccgac gtcaacaaca ctccttcgga ggcaacatgt cagacaaaga cttaacccag
     4381 cccccagcag gcgagtttat tatgtttgcc agcggtgacg gaagagtgcg tgttgaatgt
     4441 cgctttgagt cagacacaat ctggttatcc caggcggcaa tggctgaact gtacgataaa
     4501 gatgttcgca ctattaacga acatttaatc aatatatttt ccgaaggtga acttgttcaa
     4561 aactcaacca tccggaaatt ccggatagtt cgccaggaag gaaaacgtca ggtttccaga
     4621 gagatagacc attacaacct cgaagccatt ctcgctgtcg gctatcgcgt tcgctcgcct
     4681 cgaggcacgc aattccgcca gtgggccacc cagacgcttc aggaatacct gatcaaaggt
     4741 tttgtgatgg acgatgagcg gctgaagaat ccgcctgtcg gttcatcggt tgtgcccgac
     4801 tattttgatg agatgcttga acgcatccgc gatattcgcg ccagcgagcg tcgggtatat
     4861 ttgcgggtgc gggagatctt tgctttagct gccgactatc agccatcgct taaagaaacc
     4921 acgcaatttt ttcaaaccat ccagaataaa ctgcattttg cctgtaccgg gcataccgct
     4981 gcggaactca tccacaaacg agcagatgcc agccagccgc acatgggctt gaccagttat
     5041 aaaggtgaag aggtacgtaa gggcgacgtg acggtggcaa aaaattatct cacccaggat
     5101 gaggtcagcg aacttaatcg cgtggttaat atgtggctgg actttgccga ggatcaggcc
     5161 cgtcgtcgtc agcaagtctt tttgcgcgac tggcaggata agctggatca gttcctgcaa
     5221 tttaacgacc gagaggttct acagggcgct ggtaaaatca gcaagaaaat ggccgatgaa
     5281 aaagcgcagg cggagtatgt tcagtttgcc gaacaacaac ggcgcttaaa agaagccgaa
     5341 ggcgagaagg atatcgctgg tttgctacag tggaataaag agtcgaaaaa gtagcccgga
     5401 atccccccaa acgccatacg caaaaccatt tgggggtact tatggggtta ctcatttatc
     5461 aagttcaaaa tataaacatt aaatcaataa cttaaccatc aaatgcgact cctgtgatcg
     5521 ccaaaatgcc tcttcagtga tccccatcct gcttatttct gagcaacctg accctgctga
     5581 tgtatctggt agtatgacag agtgattcat ttttcttgcc gagtgtacga aaacgtcttc
     5641 cttgccaccg accatcaacc gtcactcaaa aaaacctctc ttttttcaag ctatcaagaa
     5701 taagttgcat tttatctgca ccggacacca gccatccata tatggctttt acctgctaca
     5761 aagatg
//
LOCUS       DACADF010000226         5477 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.228, whole genome shotgun sequence.
ACCESSION   DACADF010000226 DACADF010000000
VERSION     DACADF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.228"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(24..560)
                     /gene="ssb1"
                     /locus_tag="ISB30_004543"
     CDS             complement(24..560)
                     /gene="ssb1"
                     /locus_tag="ISB30_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAP1992100.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            815..3637
                     /gene="uvrA"
                     /locus_tag="ISB30_004544"
     CDS             815..3637
                     /gene="uvrA"
                     /locus_tag="ISB30_004544"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP1992101.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPDGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3672..4028)
                     /locus_tag="ISB30_004545"
     CDS             complement(3672..4028)
                     /locus_tag="ISB30_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAP1992102.1"
                     /translation="MTILELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4032..4448)
                     /locus_tag="ISB30_004546"
     CDS             complement(4032..4448)
                     /locus_tag="ISB30_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP1992103.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4559..5272)
                     /gene="aphA"
                     /locus_tag="ISB30_004547"
     CDS             complement(4559..5272)
                     /gene="aphA"
                     /locus_tag="ISB30_004547"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HAP1992104.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1299 a         1445 c         1439 g         1294 t
ORIGIN      
        1 ttacaaatta atatttatca gggctagaac ggaatatcgt catcaaagtc catcggcggc
       61 tcgttagacg gcgctgccgg agcggactgc tgcgggcgag actgcgcgcc gccgctgaac
      121 tgattgccac cctgcggctg ctgaggctga ccccaaccgc cctgcggctg accaccaccg
      181 atattgccac ctgcaggagc gccaccaccc tgacgaccac ccagcatctg catggtgccg
      241 ccaacgttca ccacgacttc tgtggtatag cgatcctgac cggattgatc ggtccatttg
      301 cgggtacgca gctgaccttc gatataaacc tgagaacctt tacgcagata ttcgctggct
      361 acttctgcca gtttgccgaa cagcacaacg cggtgccatt cagtctgctc tttcatctcg
      421 ccggtggctt tatcacgcca ggattcggaa gtagccagcg taatgttggc aactgcgcca
      481 ccatttggca tgtagcgtac ttccgggtcc tgacccagat taccaacgag aataaccttg
      541 tttacgcctc tgctggccat gttcgtgtct cctgaaaaaa aatcgttctg aataagtgta
      601 aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga
      661 aacaaacctg gccagacatt gttacacaac actccgggta atgcattcca atactgtata
      721 ttcattcagg ttaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca
      781 ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc
      841 ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt
      901 gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg
      961 gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga
     1021 gaagccggac gtcgaccata ttgaggggct ttctcctgcc atctcaattg agcagaaatc
     1081 gacgtctcat aacccgcgct ctacggtggg gacaatcacc gaaatccacg actatttgcg
     1141 tctgctgtac gctcgcgttg gtgagccgcg ctgcccggac cacgatgtac cgctggcggc
     1201 gcaaaccgtc agtcagatgg tggataacgt gttgtcccag ccggacggca agcgtctgat
     1261 gctgctcgcg ccaatcatta aagagcgcaa aggcgaacac accaaaacgc tggagaacct
     1321 ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc
     1381 gccgaaactg gaactgcaaa agaaacatac cattgaagtg gtggttgatc gcttcaaagt
     1441 gcgtgacgat cttacccaac gtcttgccga gtcgtttgaa accgcgctgg agctttccgg
     1501 tggtaccgcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc
     1561 caactttgcc tgcccgattt gcggttacag tatgcgcgag ctggaacctc gcctgttttc
     1621 atttaacaac ccggcgggag cctgcccgac ctgtgacgga cttggcgtac agcaatattt
     1681 cgatcctgac cgcgtgatcc agaatccgga attatcgctg gctggcggtg cgatccgtgg
     1741 ctgggatcgc cgcaacttct attatttcca gatgctgaaa tcgctggcag atcactataa
     1801 gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta
     1861 cggttctggc aaagaaaata ttgaattcaa atacatgaac gatcgtggcg atacctccat
     1921 tcgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacgga
     1981 atccagcgcg gtacgtgaag aattagccaa gtttatcagc aatcgtccgt gcgccagctg
     2041 cgaaggaacc cgtctgcgtc gggaagcacg ccacgtgtat gtcgagaata cgccgctgcc
     2101 cgccatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactcgc
     2161 aggtcagcgg gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt
     2221 cctcgttaac gtcggcctga attacctgac actttcccgc tcagcagaga cactttccgg
     2281 cggtgaagcc cagcgtattc gtctggcgag ccagattggt gcaggcctgg ttggcgtgat
     2341 gtacgtgctg gatgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgctggg
     2401 tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc acgacgaaga
     2461 cgcgattcgc gccgctgatc atgtgatcga tatcggtccg ggtgcgggtg ttcacggcgg
     2521 tgaagtcgtc gcggaaggtc cgctggaagc aataatggcg gtgcctgagt cgttgaccgg
     2581 gcagtacatg agcggtaaac gcaagattga ggtgccgaag aaacgcgttc cggcaaatcc
     2641 ggaaaaagtg ctgaaactga cgggcgcacg cggcaacaac ctgaaagacg tgacgctgac
     2701 gctgccggtg ggtctgttta cctgcattac cggagtttca ggttccggta aatcaacgct
     2761 gattaacgac acactgttcc caattgccca acgccagttg aatggtgcga ccatcgccga
     2821 accagcaccg tatcgcgata ttcaggggct ggagcatttc gataaagtga tcgatatcga
     2881 ccaaagccca attggtcgta ctccgcgttc taacccggcg acctataccg gcgtgtttac
     2941 gcctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg
     3001 acgtttcagc tttaacgttc gcggcggacg ctgcgaagcc tgtcagggcg acggcgtgat
     3061 caaagtggag atgcacttcc tgccggacat ttacgtaccg tgcgaccagt gcaaaggcaa
     3121 acgctataac cgtgaaacgc tggagattaa gtacaaaggc aaaaccatcc acgaagtgct
     3181 ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcacgtaa
     3241 gctgcaaaca ttgatggacg ttggtctgac gtatattcgt ctgggtcagt ccgcaaccac
     3301 actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac
     3361 cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca
     3421 gcaactgctc gacgtactgc ataaactgcg cgatcagggc aataccattg tggtaattga
     3481 gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg
     3541 cagtgggggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcag agtgcgaagc
     3601 ttcgcatacg gcgcgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg
     3661 gccttcttct ttcagagttg caccagcaat ttacgttttt cttccggcag caaattcacc
     3721 gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag cgatccgtcg
     3781 agatacacgg tgctccagtg tgctttgttc agatggcggc ttggacgcac atcgctgtgc
     3841 tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt
     3901 tctacttctt tcaccatcgc aaacagcaca tcttccactt tgatctgcgt cgctttccag
     3961 tcgttatgca cgctctgttc tgcgcctggt tttgccatgc aatattgtag caactccaaa
     4021 atggtcattt tttactcccc ttgaagtgtc gcgatgatgc gacgcgatcc gccgtggatg
     4081 cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgcacc
     4141 ggcaatacaa gcgatgttcc cagcattgag gatttgatat gagaaggcat atcgtctgct
     4201 ccctcatagt catgctcata atttccgttg tcgggaacgg cgcggaggaa aaaacgctcc
     4261 atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt
     4321 tgcagcaaca gatgcagtaa accgatgtta acgcgcggca tatcagccag ctgattcaga
     4381 atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga
     4441 taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa
     4501 ggataaaggt gtgattaaaa aaacagcatt cgggagagta ttacgctctc ccgctctgtc
     4561 agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt
     4621 aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccaacatcg cgtgcggcgg
     4681 taatatcgtt atcggaatca ccataaaaaa ttcggatatt tttatcctgc agccattgcg
     4741 attttgtatt ttgacctggt ttatcgcccg caaagatcac cggattcatg ttggtggcag
     4801 gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttggtc gggctacgac
     4861 cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga
     4921 cctcttttgg aatgctgaat tcatcccagc cattgttcat tttttcccag aacacaggat
     4981 ttttcagata atcttcgctt tctggtgaga aggttttttt gccgcgccag aaacccggac
     5041 tggaaaaaag taccgtatca tcgatatcaa accccaccgc cattggcgga cgtcctgcga
     5101 ggctattttc aatttgtgca accgaaaccc aatgaattgg tgcctgttca gcgagtttgg
     5161 ctacgttagt acccggatta agcggtgaag gagatgaggc cagggcaaca gcggaactgt
     5221 ttagagcgaa caataagcaa acggcactca atgcctgtgt gatcttgcgc atatttttcc
     5281 ctaaatagtc agtaagttga aacttattat tgtgcgtttg ttgaaaaaac catctgacca
     5341 taacgccaac cgcaggggaa aggaaggatt atctgcggat ttgtcacata aaaagagcat
     5401 gatcacattt ggaatacctg tgcgataaac cacacattgc cacggttatt ctgcttacac
     5461 attcacacaa tggcact
//
LOCUS       DACADF010000227         5424 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.229, whole genome shotgun sequence.
ACCESSION   DACADF010000227 DACADF010000000
VERSION     DACADF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.229"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            40..558
                     /locus_tag="ISB30_004548"
     CDS             40..558
                     /locus_tag="ISB30_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1992105.1"
                     /translation="MSSAAVSAAEGDESVTTTVNGGVIHFKGEVVNAACAIDSESMNQ
                     TVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAFLGTTVTSNDDTLALQ
                     SSAAGSAQNVGIQILDRTGEVLVLDGATFSAKTDLIDGTNILPFQARYIALGQSVAGT
                     ANADATFKVQYL"
     gene            920..1630
                     /locus_tag="ISB30_004549"
     CDS             920..1630
                     /locus_tag="ISB30_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP1992106.1"
                     /translation="MQTTRTPYSVSFMATVLLLFLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTHLAMAPEEAPA
                     MLRFRRSGSILTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAVTGDISF
                     QTINDYGALTPLIKATMQ"
     gene            1672..4323
                     /locus_tag="ISB30_004550"
     CDS             1672..4323
                     /locus_tag="ISB30_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP1992107.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTFALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMASRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSADKHVPDNSACTPLQDRLADASSEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFVQASLMHGLKGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTSEQTLFIDYFNLFYSKRGQEQISISQQLGHYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTQGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPAVSPGTLLNQQTAICR"
     gene            4337..4867
                     /locus_tag="ISB30_004551"
     CDS             4337..4867
                     /locus_tag="ISB30_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP1992108.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHTGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVSASATFTLEFQ"
     gene            4880..5383
                     /locus_tag="ISB30_004552"
     CDS             4880..5383
                     /locus_tag="ISB30_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP1992109.1"
                     /translation="MKRLHKRFLLATFCTLFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPIETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYIWQ"
BASE COUNT         1543 a         1178 c         1206 g         1497 t
ORIGIN      
        1 ctcaaacatg tcggtattat tgtcgtttct gtgttggcga tgtcgtctgc tgcggtaagc
       61 gcagccgagg gtgatgaatc agtaacgacc actgttaatg gcggtgtaat tcattttaaa
      121 ggtgaagtgg taaatgccgc ttgtgcgatt gattcagaat caatgaacca aacggttgag
      181 ctgggtcagg ttcgttcttc tcgcctggct aaagcgggtg acctcagctc cgccgttggc
      241 ttcaatatca agctgaatga ttgtgatacc aatgtttcca gtaatgcagc tgttgcattc
      301 ctgggtacta ctgtcaccag taatgacgat acattagcgc tgcagagttc agcggcaggc
      361 tctgcccaaa atgtcggtat ccaaattttg gaccgtacag gggaggtatt agtacttgat
      421 ggtgctactt ttagcgctaa aactgacttg attgatggca cgaatatact gccgttccag
      481 gctcgttata ttgctctcgg gcagtcagta gctggtactg caaatgctga tgcgaccttc
      541 aaagttcaat atctataatt cactcttaag gtgactatgc gtcatctgaa attgaaacag
      601 ggtgtgtgtg gcatggctac gcacacccga gaaattcacc aatgttcatc tcattattaa
      661 tgaaaaatgg tttgtatttt ggagcggtat gaaaaatcac ataatatcta aacgatcaat
      721 aaacaacagt agttaaactt gctgttattt aaaatattta cttggtagca aggttttcct
      781 atcaaaagag aaccaacatt tattacaaac gcattaagtc tggaaacccg gcgactcgcc
      841 aatgtattag caatatttac cgattgtctg atgtaacggt ttccccgctt atcctcttaa
      901 cacatggaga atgcatcaga tgcaaactac gcgtaccccc tactctgttt cctttatggc
      961 gacagtgtta cttttatttc tctttgcttg ccatagcacc attgccaacg cagctgtcgc
     1021 acttggtgcg acgcgggtaa tttatccggc aaaccagaaa caggttttat tgcctgtcac
     1081 gaataatgat cctgccagtg tctatctcat tcagtcatgg atagaaaatg cgggtgacca
     1141 gaaagatacc cagtttgtca ttaccccacc gcttttttcc atgcaaggta aaaaagagaa
     1201 tacgcttcgg ataatcaacg ccactaatca tcagttacca ggcgaccgtg aaagcctgtt
     1261 ctgggttaat gttaaagcta tccctgctat ggaaaaagac cagaagaatg agaatacatt
     1321 acagctggca attattagcc gcataaaaat gttttatcgc cccacgcatc tggcaatggc
     1381 acctgaagaa gcgcctgcaa tgcttcggtt tcgtcgcagc ggtagcatac tcactcttat
     1441 taatccaacg ccctacttca ttaccgtaac aaatatgaaa gcaggaaata gcaatctccc
     1501 aaacaccatg gtgccgccaa aaggtgaagt atcggtggat atcccgcatg ctgtaaccgg
     1561 ggatatcagt tttcagacaa tcaatgatta cggtgcatta actccgctta ttaaggcaac
     1621 catgcagtaa gccagtgacc taattaataa taaactcaat cgggattaaa aatgacagcc
     1681 tttcgtgccg catttaaggc atatcgtatg catcaggtgt taatactgcc tcgctttgca
     1741 cggcttacct ttgcccttgg cttagcaact gcggtttttc ccgttgacgc agagtattat
     1801 tttaatccac ggtttttaag caatgacctt gctgaatctg tcgatttatc tgcctttact
     1861 aagggacgcg aggccccacc tggtacatat cgggtcgaca tttatttgaa cgacgaattt
     1921 atggctagtc gggatattac ttttattgca gatgataata acgcagatct catcccatgc
     1981 ctgagtacag accttcttgt cagtcttggc ataaaaaaat cagcattatt agacaacaag
     2041 gaacattcgg cagataaaca tgtgccggac aacagcgcat gcacaccact tcaggatcgt
     2101 ttggctgacg catcatctga gtttgatgtt ggtcagcaac atctgtcact ctccgttccg
     2161 caaatatatg tcggcagaat ggctcgcggc tatgtttctc ctgatctgtg ggaagaagga
     2221 ataaatgccg ggctactaaa ctatagtttt aatggtaatt ctattaataa ccgtagtaac
     2281 cataatgcag gaaaatccaa ctatgcatat ttgaatttac agagtggcat caacattggt
     2341 agttggcgac tgcgcgataa ctcaacgtgg agttataaca gtgggagtag caattcatct
     2401 gacagcaata aatggcagca tatcaatacg tcggctgaac gtgacattat tccgttacgc
     2461 tcacggttaa cagtgggtga tagttatacc gatggcgata tttttgacag tgtgaacttt
     2521 cgtggcctta aaataaattc aacagaagcg atgttgcccg atagccaaca tggtttcgct
     2581 ccggtgattc atggtattgc tcgtggcacc gcacaagtga gtgtaaaaca gaatggatac
     2641 gatgtttatc agactactgt cccacccggc ccttttacta ttgatgatat caactctgcg
     2701 gccaatggtg gtgatctgca agtaaccata aaagaggcag acggcagtat tcagacatta
     2761 tatgttcctt attcgtctgt tccggttctc caacgtgctg gatatacgcg ttatgcactt
     2821 gccatggggg aatatcgtag tgggaataac ctgcaaagct cccccaagtt cgtacaagca
     2881 agcttgatgc atggtctgaa aggaaactgg acaccttatg gcggaatgca aattgcagaa
     2941 gattatcagg ccttcaacct tggtattggt aaagatttag gactttttgg tgccttttct
     3001 ttcgatatca cgcaggccaa tacgacgctt gcagatgaca cccgtcacag cggacaatcg
     3061 gttaaatccg tctacagcaa atccttctac cagacgggaa ccaatatcca ggtcgcagga
     3121 tatcgctatt ctacgcaagg tttttataac ttatccgaca gtgcatacag tcgaatgagt
     3181 ggttacaccg tcaagcctcc taccggagac accagtgagc agacactatt tattgattat
     3241 tttaatctgt tctacagtaa gcgtggtcag gaacaaataa gcatctctca gcagcttgga
     3301 cattacggta cgacattttt cagtgccagt cgccaaagtt actggaacac gtcacgcagc
     3361 gaccagcaaa tatcatttgg attaaatgtg ccgtttggtg atattacgac ttcgctgaat
     3421 tacagctatt ccaataatat atggcaaaac gatcgggatc atttactcgc ttttacactt
     3481 aatgttccct ttagtcattg gatgcgtaca gacagtcagt cggcatttcg taattcaaac
     3541 gccagttaca gtatgtcaaa cgatttgaaa ggcggcatga ccaacctatc aggggtttat
     3601 ggcactctgc tgccggataa taacctgaat tatagcgttc aggtcggtaa cacccaaggg
     3661 ggtaatacat cgtctggcac cagtggttac agttctctta attatcgtgg agcttacggt
     3721 aatacgaatg tcggttacag tcggagtggt gacagcagcc agatttatta cggaatgagt
     3781 ggtgggatta ttgctcatgc tgatggcatc acctttggac agccgctggg cgacacaatg
     3841 gttctggtta aggctcctgg cgctgataat gtcaaaatag agaaccagac cggaattcat
     3901 accgactggc gtggctatgc catattacca tttgcgacag aatatagaga aaatcgtgtc
     3961 gctcttaacg cgaattccct tgcagataat gttgaactgg atgaaaccgt agtcactgtc
     4021 atcccaactc acggtgctat tgccagagca acatttaatg cacaaatcgg cgggaaagta
     4081 ttaatgacgt tgaagtacgg taataaaagc gttccattcg gtgcaattgt cactcacgga
     4141 gagaataaaa atggcagcat tgtcgcggaa aatggtcagg tttatctgac tggacttcca
     4201 cagtcaggga aattacaggt ttcatggggc aatgataaaa actcaaactg tattgtcgat
     4261 tacaagcttc ctgcagtctc tcctggaacc ttgctgaacc agcagacagc aatctgtcgc
     4321 taaggaacaa tagattatga aatacaataa cgttattttc ctcggtttat gtctggggtt
     4381 aaccacctat tctgctttat ccgcagatag cgttattaaa attagcgggc gcgttctcga
     4441 ttatggctgc acagtctcat cggattcgct taattttacc gtagatctcc aaaaaaacag
     4501 tgccagacaa tttccaacga ccggtagcac aagtcctgcc gtcccttttc agattacgtt
     4561 aagtgaatgc agcaaaggga caacgggggt gcgggttgca tttaacggta ttgaggatgc
     4621 agaaaataat actctgttga aactggatga aggaagcaat acggcctccg gtttgggtat
     4681 agaaatactg gacggaaata tgcgtccggt gaaattgaat gaccttcata ccgggatgca
     4741 gtggatccca ttggtaccag aacagaacaa tattttgcct tactccgctc gtttgaagtc
     4801 aactcagaag tccgtcaatc caggactggt gagcgcttcg gcaaccttta cccttgaatt
     4861 tcaataatca gagtcagata tgaaaagact tcacaagagg ttcctgttag ctacgttttg
     4921 cacgttattt acagcaactc tccaggccgc cgatgtcact atcactgtta atggtcgggt
     4981 agtcgctaaa ccatgcacta ttcaaaccaa agaagctaac gttaatctcg gggatcttta
     5041 tacacgcaat ctgcaacaac ctggttctgc atctggctgg cacaatatta ctttgtcatt
     5101 aaccgattgt ccgattgaaa caagtgcagt gacggcaatc gtgacaggtt caactgacaa
     5161 tacgggttat tacaaaaatg aaggtactgc cgaaaatatt cagatagagc taagggatga
     5221 ccaggatgcg acgttaaaaa atggcgatag caaaacggtt attgttgatg agatcactcg
     5281 taatgcacag tttccactta aggcaagagc tatcacggtg aatggaaacg caagccaggg
     5341 aacgatcgag gcgctaatca atgtgatcta tatctggcaa taacatcaat ttgattttat
     5401 ttgcctcctt tttaaatcaa tttt
//
LOCUS       DACADF010000228         5370 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.230, whole genome shotgun sequence.
ACCESSION   DACADF010000228 DACADF010000000
VERSION     DACADF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.230"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            113..1471
                     /gene="mltD"
                     /locus_tag="ISB30_004553"
     CDS             113..1471
                     /gene="mltD"
                     /locus_tag="ISB30_004553"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAP1992110.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1520..2290)
                     /locus_tag="ISB30_004554"
     CDS             complement(1520..2290)
                     /locus_tag="ISB30_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP1992111.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGQDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYVE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRVLKPGGLLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHIRNHASGEWLTLISEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMLEAHKAA"
     gene            complement(2368..3168)
                     /locus_tag="ISB30_004555"
     CDS             complement(2368..3168)
                     /locus_tag="ISB30_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP1992112.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3409..4323
                     /locus_tag="ISB30_004556"
     CDS             3409..4323
                     /locus_tag="ISB30_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1992113.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELIADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4320..5123)
                     /gene="dkgB"
                     /locus_tag="ISB30_004557"
     CDS             complement(4320..5123)
                     /gene="dkgB"
                     /locus_tag="ISB30_004557"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAP1992114.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELHITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHSIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5286..5362)
                     /locus_tag="ISB30_004558"
     tRNA            complement(5286..5362)
                     /locus_tag="ISB30_004558"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5326..5328),aa:Asp,seq:gtc)
BASE COUNT         1408 a         1301 c         1338 g         1323 t
ORIGIN      
        1 aacgttttgc atggttaagg tcaaagaaag ataggttctg ataaaacttt cttgtcatcg
       61 gctccgttcg ccgttatgat cggtcgtctt ttaagcaact attgacacac acatgaaggc
      121 aaaagcgata ttactcgcct ctgtcctgct cgtgggttgc cagagcaccg gcaacgttca
      181 acagcacgca cagagccttt ctgcagctgg tcaaggggaa gcagcaaagt ttacaagtca
      241 ggcacgatgg atggacgatg ggacgtctat cgcgccagat ggtgacttgt gggctttcat
      301 tggcgacgag ctaaagatgg gaattccgga aaacgaccgg attcgcgaac agaaacagaa
      361 atatttacgc aataagagct atctccacga tgtaacttta cgggcagagc cgtatatgta
      421 ctggatagcc gggcaagtta aaaaacgtaa catgcctatg gaactggtac tactacccat
      481 agtggagagc gcttttgatc ctcacgcaac gtctggcgcc aatgccgcgg gcatctggca
      541 gatcattccg agcacggggc gcaattatgg tttgaaacag acccgcaatt atgacgcgcg
      601 tcgcgatgtt gttgcttcaa caactgccgc gctgaacatg atgcagcgtc tgaacaagat
      661 gtttgacggc gactggcttc tgaccgtagc ggcttataac agcggcgaag gtcgggtcat
      721 gaaggcaatt aaaacgaaca aagcgcgtgg gaaatccacg gacttctggt cgttaccgtt
      781 gccgcaggaa acgaagcagt acgtgcctaa aatgctggca ttgagtgata ttctcaaaaa
      841 cagcaagcgt tatggcgtac gtctgccaac gaccgatgaa agccgtgctc tggcacgtgt
      901 gcacctgagc agcccggttg aaatggcgaa ggttgcagat atggcgggga tttccgtcag
      961 caagctgaag acattcaacg caggcgtgaa aggctccacg ctgggcgcaa gtggtccgca
     1021 gtacgtgatg gtgccaaaga agcatgcaga tcaactgcgt gaatctctgg cttcaggcga
     1081 aattgctgct gtacagtcga cgctggttgc cgacaatacg ccacttaaca gccgtgttta
     1141 caccgtacgc tctggcgaca cgctttcaag tatcgcttca cgtctcggcg taagcaccaa
     1201 agatttgcag cagtggaaca aactgcgcgg atctaagctg aagccaggcc aaagtttgac
     1261 gattggtgca ggcagtagcg cacagcgact ggcaaacaac agcgatagca ttacgtatcg
     1321 tgtgcgcaaa ggcgattcgc tttcaagcat tgctaaacgc cacggcgtga acatcaaaga
     1381 tgtaatgcgc tggaacagcg acactgcgaa tctgcaacca ggcgataagc tgacgttgtt
     1441 tgtgaaaaac aacaacatgc cagattcctg acaaaccaga taataaaaaa ggcaccgatt
     1501 cccccggtgc cttttttatt tatgccgctt tatgtgcttc tagcatgatg atatcactgg
     1561 tgaaagagcc atcattctgc aaggcaaaat acgttttcac ctctgtcgat gcgctctgtt
     1621 ggtaaatgcg aatagcgtct actaacgctt ctggcgtacg cattctcgct acccatgaag
     1681 aaaattccag cggtaactta tctgtaatta aattatcaac tatcagattg gcttcactga
     1741 ttaacgtcaa ccactcaccg ctggcgtggt tccgtatgtg agatgtatcg cgtaatgctt
     1801 ctaccgtctg taaccagatg tcgcgcactg ggtgacccgg agacattacg tccatcacaa
     1861 tcagcagacc accgggtttc aatacccggt tcacttcacg caatgctgca ccaacatcat
     1921 gccaatgatg ggcagaataa cggctgataa caatatcaaa tgcgttatcg gcaaatggca
     1981 ggctttcgac ataaccctgg cgggtggtga tatttttcag ttgccgggct tcggcagctt
     2041 gtgccacgac atccagcata tgggcagata agtcatacgc caccaccgtg ctcacgtttt
     2101 gcgcagcgac aaagctggca tgccctgctc cgcagcccat atcaagtaca cttgcatcag
     2161 gataatcagc cagacgcacc gccagacgct gtaaatcttg tccggatgca tgtacggtac
     2221 tggtcagata ttcgctggct tgagagctaa actgcttttc tacgtggtca tggtgggatt
     2281 gtgttgtcat tgcgctgtcc ttttgttatt tgagtataaa agggcagcgc caaacgttgg
     2341 tctgccctgt ggcagacctg acatacctta tttatcaggc ttgccgggac tgaattcaac
     2401 gagtagcgga ttgtgatcgg acgcgcgcgt aaccagtaca gaagcttcgc tgacgttcag
     2461 accacggtag aaaacaaaat cgagcgggcg accaaacgcc cgacggcgct gatcatcggt
     2521 aaaacgcacc tggcgcagcg acatttcccg cgcaaagcga tataacgcgt tcatccttct
     2581 acggctccag gcattgaaat ctcccgccat aatgaccggg ccactgtggt gagctatctg
     2641 atcgccaata ggaagtaact gcttactata gacatccacg cccagactga agttgacggc
     2701 gtgtatatta accaccatca acaggcgggt gtcaggtaac ggatagaccg tcaccagtgc
     2761 cgacttcgcc agacgcaaaa tgggttctcg ttcacgtaac gggcagcaat acactggatg
     2821 tgccgccgaa agggtcatta cgccagaagg atgttgtggc agcacgaaag cgggtacctg
     2881 atcggcggca agatagttag cagtcgcaaa ctgtactaac tctggcgttg tctgcgcttc
     2941 ctgcaataac accagatgtg catctttgcc gtagttcttt aataccgaca accattcagc
     3001 gcgttgctgt ttgtatatgt tccacaccag gatccgaata cgctcttcgg tacttaacgg
     3061 ttctccaggt ggtaatgcct ggccgatgct cgcaaaagac cccggcggta agatcctttc
     3121 cgcaggttgt ccggcaacat agcgcatggc ataggtgttt tttcgcactt ttgactttga
     3181 aacctctatc acgtcaaccc ttccgcactc gggagggttc tactgttata gggattttag
     3241 ctcactcttt caacgagcaa ttgtatattg ttatgtaagc aagtgcttac accggtcagg
     3301 attttgctca ttattgagaa cattaaatcg gatggcatat ctcgcatcct gatggtttaa
     3361 tgacaaataa attcaaaata tctcttctat tttgcaacag gagcaaaaat gaaagccacg
     3421 tcggaagaac tcgccatttt tgtttcagtc gtcgaaagcg gcagctttag ccgggcagcg
     3481 gaacaattag ggcaagcaaa ctcagcggta agccgggcgg tgaaaaagct ggagatgaaa
     3541 cttggcgtta gcctgcttaa tcggaccacg cggcaactta gcctgacgga agaaggcgag
     3601 cgttattttc gtcgcgtaca gtcaattttg caggagatgg cagcggcaga atcagaaatt
     3661 atggagacgc gtaatacacc gcgtggactg ttacggatcg atgccgcaac tccagtggtg
     3721 ctgcactttc tgatgccgtt gattaagcct tttcgtgaac gctatccgga agtcacttta
     3781 tcgctagtct cctccgaaac gattattaat ttgatcgaaa gaaaagtgga tgtcgcgata
     3841 cgcgccggta cgttaacgga ttccagctta cgtgccaggc cgctatttaa cagttatcga
     3901 aaaattatcg cctcccccga ttatatttcc cgctacggga agccagaaac gatcgacgat
     3961 ttaaagcaac atatttgcct gggattcact gaacccgctt ccctcaatac ctggccgata
     4021 gcctgtagcg atggacaatt acatgaggtt aagtacggtt tgtcatccaa tagtggggaa
     4081 acactgaaac agctttgcct gagtggcaac gggattgcgt gtttgtccga ctacatgatc
     4141 gacaaagaaa tcgctcgcgg agaattggtg gagttaatag cagataaagt gttgccagta
     4201 gaaatgccat tcagtgcagt ctattacagc gaccgtgcgg taagtacgcg catccgggct
     4261 tttatcgatt tccttagcga gcatgtaaaa acagctcccg gaggagctgt cagagaggct
     4321 taatcccatt caggagccag accttccggg ctaaccaggc ggtcgttgca atccagtgcg
     4381 gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt gtgccttaag attactctcc
     4441 aggttttcac gtttagtaga agaaggaatt actgagtaac cttcccccat agcccacgcc
     4501 agaatcactt gagccggagt cgcattgtgt ttagctgcaa tacgagcaat aacctcatct
     4561 ttcagtgcct tgccatacgc cagcgtcata taggaagtaa tatggatact gtgctgttta
     4621 gcccaagcga ccactttacg gttttgcaga taaggagaga gttcaatctg gttagtagcg
     4681 atgttttcag cgccaacagc agcaatcgct ttttccatca atgggatcgt aaagttggaa
     4741 ataccgatct cacgcgtcag cccttgtttt ttggcttcca gcagcgcctg cataaactct
     4801 tcaacagaga cttcatcgtt tggtgacggc cagtggatta gcgtcagatc aacataatcg
     4861 gtacgcaatt tttgcaggct ctctttcaga ctcgggatca atttgtcttt gctgagattt
     4921 tcaatccaga ttttagtggt gatgtggagt tcatgacgtg gcacgccact ttctgcaatc
     4981 gcctgaccta ctgcggcttc gttatcatag atttgtgcgg tatcaattgc gcgataatca
     5041 agttcaagcg ccgttttcac agatgaaata acaacgtcgt ctttcagacg gaaagtgcct
     5101 aaaccaaatg cagggatagc cataatatgc ctcttttaat gctattttcg ttaactgagg
     5161 tgattatggg cttcagactg ttgcggaaaa atggcgtaaa acgcagagga tttttgcgat
     5221 tttggcaata atagaaatac aaacgaaaaa aaacctcgag cgttaactcg aggcttctta
     5281 gtaagtggcg gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc
     5341 taaccgactg aactaccgct ccaccgaatt
//
LOCUS       DACADF010000229         5291 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.231, whole genome shotgun sequence.
ACCESSION   DACADF010000229 DACADF010000000
VERSION     DACADF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.231"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            1..1335
                     /locus_tag="ISB30_004559"
     CDS             1..1335
                     /locus_tag="ISB30_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1992115.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1351..2121
                     /gene="garL"
                     /locus_tag="ISB30_004560"
     CDS             1351..2121
                     /gene="garL"
                     /locus_tag="ISB30_004560"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAP1992116.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2151..3041
                     /gene="garR"
                     /locus_tag="ISB30_004561"
     CDS             2151..3041
                     /gene="garR"
                     /locus_tag="ISB30_004561"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAP1992117.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETAATAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3138..4283
                     /gene="garK"
                     /locus_tag="ISB30_004562"
     CDS             3138..4283
                     /gene="garK"
                     /locus_tag="ISB30_004562"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAP1992118.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEALRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4316..4692
                     /gene="rnpB"
                     /locus_tag="ISB30_004563"
     ncRNA           4316..4692
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ISB30_004563"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1241 a         1250 c         1486 g         1314 t
ORIGIN      
        1 atgattctgg acaccgttga cgtaaaaaag aaaggcgtgc atacccgcta tttaatatta
       61 ctgattattt ttattgttac cgccgttaac tacgccgatc gtgcaacgct gtctattgct
      121 ggtaccgaag tggcaaaaga gttgcagtta agtgcggttt cgatgggtta catcttctct
      181 gcttttggct gggcctactt gctgatgcaa attcccggcg gctggctgct tgataagttt
      241 ggctcgaaga aagtttacac ttacagcctc tttttctggt cgctgttcac cttcctgcaa
      301 ggctttgtcg atatgtttcc gctggcctgg gctgggatct ccatgttctt tatgcgattt
      361 atgctggggt tctcggaagc gccatcattc ccggcgaacg cccgtattgt cgccgcctgg
      421 ttcccgacga aagaacgtgg tactgcctcc gccatcttta actcggcgca atatttctcg
      481 ttggcgctct tttcgccgct gcttggctgg ctgactttcg cctggggctg ggagcatgtc
      541 tttaccgtta tgggggtgat tggttttgtg ttgacggcgc tgtggatcaa gttgattcat
      601 aacccgacag atcacccacg tatgtctgca gaagagctga agtttatctc ggaaaatggc
      661 gcggtggtcg atatggacca caaaaagccg ggtagtgcgg cagcaagcgg acccaaactg
      721 cattacatca agcaattgct ctctaaccgc atgatgctgg gcgtattttt cggacaatat
      781 tttatcaaca ccatcacctg gttcttcctc acctggttcc caatttatct ggtgcaggaa
      841 aaaggcatgt cgattctgaa agtgggtctg gtcgcctcga ttccggcact gtgtggcttt
      901 gcgggcggcg tgctgggagg tgtcttctcg gattatctga tcaaacgcgg tttgtccctg
      961 accctggcac gtaagctacc aattgtgctg ggaatgttgc tggcttccac catcatctta
     1021 tgtaactaca ccaacaacac cacgctggtg gtcatgctga tggcgctggc tttctttggc
     1081 aaaggatttg gtgcgctggg ctggccagtg atttctgaca ccgcgccgaa agagattgtt
     1141 ggcctctgcg gcggcgtctt taacgtcttt ggcaatgttg cctccattgt cactccactg
     1201 gtgattggct atctggtaag tgaactgcac tccttcaatg cagcactggt tttcgtggga
     1261 tgttcagcgc tgatggcgat ggtctgctac ctcttcgtag tcggcgacat taaacgtatg
     1321 gaattgcaga aataagcaaa ggtataagcg atgaataacg atgttttccc gaataaattc
     1381 aaagccgcac tggctgcgaa acaggtacaa attggttgct ggtcagctct ctctaacccg
     1441 attagcactg aagttcttgg tttggctggg tttgactggc tggtgctgga tggtgaacat
     1501 gcgccaaacg atatctccac gtttattccg cagttaatgg ccctgaaagg cagcgccagc
     1561 gcgccagtag tgcgagtgcc gaccaacgag ccggtaatta ttaagcgtct tctggatatc
     1621 ggtttctata acttcctgat tccttttgta gaaacaaaag aggaagcaga gcaggcggtg
     1681 gcatcaactc gttacccacc ggaaggcatt cgcggcgtct ccgtttctca ccgcgccaat
     1741 atgtttggca ccgtggcgga ttatttcgct cagtcgaaca agaacatcac tattctggtc
     1801 cagatagaaa gtcagcaggg cgtagataac gtcgatgcta ttgccgctac cgaaggtgta
     1861 gacggcatct tcgtcggccc cagcgatctg gctgcggcat taggccatct cggcaatgcc
     1921 tcgcacccgg atgtacaaaa agcaattcag cacattttta accgtgccag cgcgcacggc
     1981 aaacccagcg gcatcctcgc accggtcgag gctgatgcgc gtcgttatct ggaatggggc
     2041 gcgacgtttg tggccgtcgg cagcgatctc ggcgtcttcc gctctgccac tcagaaactg
     2101 gctgatacct ttaaaaaata accaccaccg caacaagaga gatgattgat atgactatga
     2161 aagttggttt tattggcctg gggattatgg gtaaaccaat gagtaaaaac cttctgaaag
     2221 caggttactc gctggtggtt gctgaccgta acccagaagc tattgctgac gtgattgctg
     2281 ccggtgcaga aacagccgct acggctaaag cgatcgctga acagtgcgac gtcatcataa
     2341 ccatgctgcc aaactcccct catgtgaaag aggtggcgct gggtgagaat ggcattattg
     2401 aaggcgcgaa gccaggtacg gtattgatcg atatgagttc tatcgcaccg ctggcaagcc
     2461 gtgaaatcag cgaagcgctg aaagcgaaag gcattgatat gctggatgct ccggtgagcg
     2521 gcggtgaacc gaaagccatc gacggtacgc tgtcagtgat ggtgggcggc gacaaggcta
     2581 ttttcgacaa atactatgat ttgatgaaag cgatggcggg ttccgtggtg cataccgggg
     2641 aaatcggtgc aggtaacgtc accaaactgg caaatcaggt cattgtggcg ctgaatattg
     2701 ccgcgatgtc agaagcgtta acgctggcaa ccaaagcggg cgttaacccg gatctggtgt
     2761 atcaggcaat tcgcggtggg ctggcgggca gtaccgtgct ggatgccaaa gcgccgatgg
     2821 tgatggaccg caacttcaaa ccgggcttcc gtattgatct gcatattaag gatctggcga
     2881 atgcgctgga tacttctcac ggcgtcggcg cgcaactgcc gctcactgct gcggttatgg
     2941 agatgatgca ggcactgcgt gcagatggtt taggaacggc ggatcatagc gccctggcgt
     3001 gctactacga aaaactggcg aaagtcgaag ttactcgcta atgacgtcgt gcccggatgg
     3061 cgtattgcaa tccgggcctg gaatccaggc cgaataagag aaacgccctc cggcgtcatg
     3121 tggtaacagg cataggtatg aaaatcgtaa tcgccccaga ctcttataaa gaaagtttat
     3181 ctgccagcga ggttgcgcag gcgatagaaa aaggatttcg ggaaattttt cctgatgcac
     3241 agtatgtttc tgttccggtt gccgacggtg gtgaaggaac ggtggaagcg atgattgcag
     3301 ccacccaggg ggctgaacgt cacgcctggg ttacagggcc gctgggcgag aaagtgaatg
     3361 ccagttgggg gatctccggc gatggcaaaa ccgcgtttat cgaaatggcg gcggccagtg
     3421 ggctggaact ggtacctgcg gaaaaacgcg atccactcgt gaccacttca cgcggtactg
     3481 gtgagttaat cctgcaggcg ctggagagcg gtgcgacaaa cattattatc ggcattggcg
     3541 gcagcgccac caacgacggc ggcgcaggca tggtacaggc gctgggggcg aaattatgcg
     3601 atgccaacgg caatgaaatt ggttttggcg gcggtagtct taatactctg aatgatattg
     3661 atatttccgg tctcgatccg cgcttaaaag attgtgtcat ccgcgtcgct tgtgatgtca
     3721 ccaacccgtt agtcggcgac agtggtgctt cgcgcatctt tggcccacaa aagggagcca
     3781 gtgaggcgat gattgtcgag ctggacaata acctctctca ctatgccgat gttattaaaa
     3841 aagcgctgca tgttgatgtg aaagatgtcc ccggcgcagg agctgcggga ggtatgggcg
     3901 cggcgctgat ggcgtttctt ggtgcagaac tgaaaagtgg tattgaaatc gtcactacgg
     3961 cgctgaatct ggaggaacat attcacgatt gtacgctggt gatcactggt gaagggcgta
     4021 ttgatagcca gagtattcac ggcaaggtac cgattggtgt cgcaaacgtg gcgaagaagt
     4081 accataaacc ggtgattggc attgcgggta gcctgaccaa tgatgttggc gttgtacatc
     4141 agcatggcat tgatgcggtc ttcagcgtac tgaccagcat tggtacgtta gacgaagcac
     4201 tccgcggagc ttatgacaat atctgccgtg cttcacgtaa tatcgccgcg acactggcga
     4261 ttggaatgcg caacgcgggg tgacaagggc gcgcaaaccc tctatactgc gcgccgaagc
     4321 tgaccagaca gtcgccgctt cgtcgtcgtc ctcttcgggg gagacgggcg gaggggagga
     4381 aagtccgggc tccatagggc agggtgccag gtaacgcctg ggggggaaac ccacgaccag
     4441 tgcaacagag agcaaaccgc cgatggcccg cgtaagcggg atcaggtaag ggtgaaaggg
     4501 tgcggtaaga gcgcaccgcg cggctggtaa cagtccgtgg cacggtaaac tccacccgga
     4561 gcaaggccaa ataggggttc ataaggtacg gcccgtactg aacccgggta ggctgcttga
     4621 gccagtgagc gattgctggc ctagatgaat gactgtccac gacagaaccc ggcttatcgg
     4681 tcagtttcac ctgatttacg taaaaacccg cttcggcggg tttttgcttt gtgtgcccag
     4741 ggtaagatga atatctacgg tattaataac agactttgta agaagttatt aagtctatat
     4801 tgcatttaaa aggtcgtaat tatacgtata tttctctcat taattgtaag ttcgcaggta
     4861 ttgtccgatc gttgaataat ttgggcgtta taacctaaat ttttaccgtc caataatcaa
     4921 tatttctatt gtgattgtta accactgcgt gcattgacgg gattgaaggg tgaagaatat
     4981 gtgcaattga gaagcctttc attgatgcct gccttgtcat catgacaaac aataatagat
     5041 gcttattctc cttgcacggt tctttcgcac tatcaaaaaa cgctcattta cccggcatta
     5101 cagtcaacga agcaacattc tatggcctac gctgatgtaa agcacagtga gaatccccct
     5161 tgttgtcttc gtctgacctt atcgcggcga ccagcaatta ttcgttttgt gatcctcaac
     5221 acaaaatgct cttcgttgcc tatgatggga gaagaattta tggaaaacga ttgcattcac
     5281 aaaatgacat c
//
LOCUS       DACADF010000230         5167 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.232, whole genome shotgun sequence.
ACCESSION   DACADF010000230 DACADF010000000
VERSION     DACADF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.232"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            1310..1519
                     /locus_tag="ISB30_004564"
     CDS             1310..1519
                     /locus_tag="ISB30_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992119.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(1585..2118)
                     /locus_tag="ISB30_004565"
     CDS             complement(1585..2118)
                     /locus_tag="ISB30_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992120.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(2121..3047)
                     /locus_tag="ISB30_004566"
     CDS             complement(2121..3047)
                     /locus_tag="ISB30_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HAP1992121.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTAPERVKSPSRGFSMGF"
     gene            3104..3622
                     /locus_tag="ISB30_004567"
     CDS             3104..3622
                     /locus_tag="ISB30_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992122.1"
                     /translation="MRFLLILRISTEDTPCTRRPQAIYCTTTTHRKKTPGSITNMRIS
                     NIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERKLLHVLATAEKNDLQAQE
                     SERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTGELCYKKDRILQALKEL
                     KYDLETSPNPDA"
     gene            complement(3826..4740)
                     /locus_tag="ISB30_004568"
     CDS             complement(3826..4740)
                     /locus_tag="ISB30_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="HAP1992123.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
BASE COUNT         1436 a         1351 c         1104 g         1276 t
ORIGIN      
        1 aaaaaaaaac cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa tattttagtt
       61 atcggatgct caatattcac aaacgcaaaa aatgcctcag tgatgcccta aaacaacctt
      121 tccagaagga gtccgcgctt tacgatgcac ggaagagata aaaaacccca caaaagtggg
      181 gaaccaagag aaacaacaat tacaggttgt tggttgtgtt cgaacaacga tgaagtagct
      241 acgctaaata ataattaccg tacaggccct acatattcaa cactaaattt aagatcctga
      301 cgaaggattt ataaatgcac gaaaacacat agacagacaa attcactaaa ttcatacaca
      361 aaaacaagtg tagttttcta agtaaattag attcgacaac caattagttt atccacatcg
      421 cattccactc ttgctacacc aatcacattt cttgatatgt aatagcagaa aaaataaaac
      481 cagacaccaa tagaccaaaa aacaaacatt caacaagaaa cattttgaac aatatcacaa
      541 aactgtcaca accatcatca gactattgaa aaaaactggt tgctgtttta taaaagcgac
      601 tgtaacaacc tccactctat agttccatta gtttaaagtg tcacacaaac tgagcatcca
      661 tttgttttcc ttacttttgc ccctgtattc caaggggttt ttttataact taaagattag
      721 cacaccacac aaaaacaata aaaaaaaacg acaagttata caataaaacc agtactatcc
      781 atacagctaa ttacatataa atactattaa accatctgga cattaaaaat aaactcttat
      841 aacataaaat taatgcggtt ccggtaccat atgtcaggga taatgtgcaa atggaggtat
      901 cggaactgca tttcagttca ttattaatta atgacaaaaa aaacctctgc gagcgcagag
      961 ggggtaagga atgttcccgg tcataatatg tcgatagtgt ttatatacat acagaacaag
     1021 aacaacaacc agccaactaa taaatatttt attttttaga gctgaaacct taaagctcac
     1081 ttccattcca tacattgtta taaaccttat gttgaaaact gaaagtaagt acggcagact
     1141 tattaagttg cgtgcagtct tttcacgtta tacaaacaag aacacctgcg aagttatttc
     1201 tccttacata tccccctatg ccaacggggg attttttctg gttcactaac gcataaataa
     1261 tagtgacacc ctatcattca caaaccgtga taatataagg attatcatca tggcaaaatt
     1321 aggtgaaaac accccacata ttatcgatgc tactgtagat tttatggcat caagccaggc
     1381 atttcgtgag tatctgaaaa aacttccccc tgataacaca ataccgccag ctcttactga
     1441 tgagcacgta ccgttatata tacgaaggct ggaatactat cgtctgcttt atcgaccaaa
     1501 gcagacagaa tcatattgaa aaaaacggtg aacggttcgc gaaattcttt cgcgggtcgt
     1561 gaggcgttgg acaagcgaag cgcgtcagtt tttccggctc ttccccgtga caggtttctc
     1621 caccagttta tcttccagtg tcacaacttt ctgatacacc gccgctgtgg cgttgtagtt
     1681 cctgtcgagt attgttttca ttccccatcc gagcacacag aacataacca gaaaagccac
     1741 tgacacccca cccagccatt tccagctata ccctttaagg gtgttgtatt cctgcacgat
     1801 attgctgtga attgtcgcaa tatccctgtt cagggcatcc gcgtttgcct tcatgtttgc
     1861 gactgactct tccagccgct gaaaatcctg gctgatgcgc tcacctatca gtcctgccac
     1921 gtccagcttc tgcacctcat ttctgacttc atccctgacc tgttttgcca caccgtcaga
     1981 aatatcccga tatccgcgaa gagtttccct tgccacagcc agagaatcag caacagcttt
     2041 ctgttgctcc attgcggtat gttgcagatg ctccaccgat aacaggagcg taagtaattt
     2101 ttcttctgtg ctggccatgt tcagaatccc atagaaaatc cacgtgacgg tgatttaacc
     2161 cgttcaggtg cagttcggtt ctgtctgctc actctgtctc tcatgtcaaa gccatgctcc
     2221 cgcgcaacat catgttgcag ccttctgacc agttcatgtt gcttaaccgg agtgtccagc
     2281 cattccctga tgtcatgccc gaccgttcgc gcctgttcaa gatggcggga atagtccggt
     2341 tcagaccgtt tttgcatgat gtcatgacgc tgggcatcat caagccgtcc ggcagcttca
     2401 aaacccagat gcacttcggg acgtcgttca atgccctgtt ctttcagcgt ccggcagtca
     2461 atgaagcccc tttcaaagcc atgctttctc atatgttcgt tgtgcttcac ctcccagcgt
     2521 tttcgcaatt cccgcaactg ctctttttgc tgagtgagag tcaggcttcc ggaatccttt
     2581 ttcgcacctc cccgctcggg atacctggcg ttatagcgcc ggaaatactg ttccggtgta
     2641 cgttcaatgc catcattcac acgctgactc atcatgatat gcgcatgagg ctgttcgccg
     2701 ccgtcaatgc tcgctttcgg attgtgaatg gcaaaactcc aggcgtgccg ttcgccagct
     2761 tcctgacgta caaaatcctg caccagttca aggcgctgtt ccggtgtcag ttcacggggt
     2821 aatgcaattt caagttccct ataggtcgaa ccgttcgcac gttcaaactg gtcagccgcc
     2881 tgccagaaat gggaaggatt atccctggcc cattccggca tgtttccgtg tgcggaatat
     2941 tccaaatcct gacggtcgcg gtatttctct ttgcgctcga tgtaatcggc gtggtttgca
     3001 gcctgcccct tgccaccaaa tttaacgctg aggtgatagg ttgccacggt ttcagccctc
     3061 tgcaggagcg cactgtttct cgacgaagga gagaaacata taagtgcgct tccttctaat
     3121 tttacgaatt agcacggaag ataccccctg cacccgccga ccgcaagcga tatactgcac
     3181 gaccacaaca caccgcaaaa aaacaccagg gagcatcacg aacatgcgca tcagcaacat
     3241 cgaatggctg aagaaacgga tcggattcat caggaagctc ggagaacaga ccgcacgaca
     3301 gagacagatt attgacctga tcgacaacga agccggactc acagaacagg aacgcaaact
     3361 tctccacgta ctggcaacag cggaaaaaaa cgacttacag gcacaggaga gcgaacgaaa
     3421 acaggcggtc caaaaacgaa tcgagggaaa aaaacaacga cgggaacgca atcaccggct
     3481 ctttctcgcg gctgggctac tgatcgaagc aggactggta gacacaaaaa caggcgagct
     3541 atgctataaa aaagacagga tattacaagc acttaaggaa cttaagtacg atctggagac
     3601 atccccaaac ccagacgcgt aagtgcctcc atccgtgttc cgattccctg gtgcttcctc
     3661 gctcattcgc tcgctacgct cggtcatgag actgcggcga gcactaccac cacaatcacg
     3721 cggaaagccg cgccattgca gggctgaacg gggaaaaacg gcaaaaaacc tgctgctcaa
     3781 aaagacgcca gaaaggcgct gttatctgat ataggcttat ttggtctact ctacgagacc
     3841 tcgttttttc agttggtaat accaggcgcg actgatccct aatgcctccc acggtttcag
     3901 cgtgcgtgca gatgtagcta ctgtgctgcg cttagactta gaaccgccct tgcgaccgcg
     3961 tgcagcctga atttctggcg tgtgcgtatc agccacatac tgtgcgaaag tttccggcgt
     4021 gaagttcctg tgcgtgtatt tcgcaatgct cttgccaata gcccgacatt cagccagtga
     4081 aagcggaacg ggaagcgatg cattgtacat ttcgacacgc tggatcaccg cttcaagcca
     4141 ctgtgagaac gctggccagc cctgacgaat cgccctgtaa gcccatttgc gcgtcatttc
     4201 gaacaggtgg cagtttcgcc ccatcccgta atgtttatcg atgctacgac gctctgaggc
     4261 gctcaaatcg agataatcag ccagttcatc gagggtatag gcttcctcgc gccactccat
     4321 caccagccat tccaggtgga acggattttt gcaaatcagg ccgctgtagt tcacatccgc
     4381 acaaagtttc tcacacagcg aacgttcaac cgctgcggca tatttcagcg ccttaaccga
     4441 ggcatcaggc gcggttctca cggcgatgtt gagtgcgtag agcaggtgag cgtgtccgtt
     4501 cacaggattt ttaacggtga tgttcggcgc gggagcgttc cggtcgctcc agtcaatcgc
     4561 agccccctcg cggtcaacgt caaaaaccag ccagaactgc gcatgaggct ggttctgctg
     4621 tatgtaccgc gcaagaagcg cacgcccttt gccggagatg cgaagaccaa aagcgagatc
     4681 atccgtgtga tagggacgat ggggtaaatt ttcgtcgaag tattgaagcg cggcgctcat
     4741 gtgttacgat tctctccggg ttatgcgacc tacacgccct ctgtatttca tgttgaatct
     4801 tgccggatct ggacatgatt tcaggggcgt ttgtttttca gtcgcttaca ctacacccaa
     4861 cggcaaatcc tgtcgaacct tcccgaaaac ccgatccatt taccataact cccgttgtac
     4921 gcccccctta aaacgccctc agtcgctttc tggcgcaaaa aacgcaaaaa gacaagaaaa
     4981 caccaggcca aaccagaaaa acgcgtcaga atcgttttca gggcgttgca taactatgca
     5041 cgaaagcgat gtgcgatcgg tttcgtgcgt tctgaacacc gaacggctca aaacgacaag
     5101 ttttctcccc tggtgcgtgg cgctctgacg gggtttcctg tttccaaact tcacatctcg
     5161 ccaggac
//
LOCUS       DACADF010000231         5135 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.233, whole genome shotgun sequence.
ACCESSION   DACADF010000231 DACADF010000000
VERSION     DACADF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.233"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            54..1850
                     /locus_tag="ISB30_004569"
     CDS             54..1850
                     /locus_tag="ISB30_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002954510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HAP1992124.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAIIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVDISYKVGNESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKAMKDDYVVQTSAIVNNYMDNRSISDLQQTKVVKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QNTFYDGRDRNINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTIGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1868..2536
                     /locus_tag="ISB30_004570"
     CDS             1868..2536
                     /locus_tag="ISB30_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HAP1992125.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2551..3222
                     /gene="dsbI"
                     /locus_tag="ISB30_004571"
     CDS             2551..3222
                     /gene="dsbI"
                     /locus_tag="ISB30_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HAP1992126.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGTLIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3268..4083)
                     /gene="ygiD"
                     /locus_tag="ISB30_004572"
     CDS             complement(3268..4083)
                     /gene="ygiD"
                     /locus_tag="ISB30_004572"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311954.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAP1992127.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLV
                     ELLAPVPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4199..4972
                     /gene="zupT"
                     /locus_tag="ISB30_004573"
     CDS             4199..4972
                     /gene="zupT"
                     /locus_tag="ISB30_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAP1992128.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
BASE COUNT         1318 a         1259 c         1266 g         1292 t
ORIGIN      
        1 acagtcgata ttcatcatta ttatatattt gcgttatata aggagatata tccatgtttg
       61 ataaatatag aaaaacactc gtagccggaa ctgtggcgat aaccctgggt ttgtcagcat
      121 cgggggtgat ggctgcgggt tttaaaccag caccgcctgc cgggcagctt ggggcgatta
      181 tcgtcgatcc ctacggtaat gcgcccttaa ccgcgctggt tgatttagac agccatgtta
      241 tttctgacgt caaagttacc gtccacggga agggcgaaaa aggcgttgac atcagctata
      301 aagtgggcaa cgagtcactg aaaacttacg atggtgtacc gatttttggt ctttaccaga
      361 agtttgcgaa caaagtgacc gttgagtgga aagaaaacgg taaggccatg aaagatgatt
      421 acgtggtgca gacttccgcc atcgtcaaca attacatgga taaccgctcc atctccgacc
      481 tacagcaaac caaagtcgtt aaagtcgcgc caggttttga agatcgcctc tatctggtta
      541 atacccacac ctttaccgcc caaggttccg atctccactg gcatggtgag aaagataaaa
      601 atgccggtat ccttgatgcg ggtccggcaa ctggcgcact cccttttgat atcgcgccat
      661 tcacctttat cgtcgatacg gaaggcgaat accgctggtg gttggatcaa aacaccttct
      721 acgatggtcg tgaccgcaac attaacaaac gtggttatct gatgggtatc cgtgaaacgc
      781 cacgcggcac ctttaccgct gtacaaggtc agcactggta cgagttcgac atgatggggc
      841 aggtgctcga agatcacaaa ctaccgcgcg gatttgctga cgccactcat gagtccattg
      901 agacgccaaa tggcacggta ctgttgcgcg taggtaagag taactatcgt cgcgatgacg
      961 gcgtacacgt caccaccatt cgtgaccata tcctcgaagt cgataaatct ggtcgcgttg
     1021 tcgatgtatg ggatctgacc aagatcctcg atccgaaacg cgatgcactg ctcggcgcgc
     1081 tggatgcagg tgcagtttgc gttaacgttg accttgccca tgcaggacaa caggcaaaac
     1141 tggaaccaga tacaccgttc ggcgacgcgc tgggtgtagg gccaggccgc aactgggcgc
     1201 acgtgaactc tatcgcctat gacgcaaaag atgactctat catcctctcc tctcgccacc
     1261 agggtgttgt gaagattggt cgtgataagc aagtgaaatg gatccttgcg ccctctaaag
     1321 gttgggaaaa accgctggcc agcaagctgc tgaaaccggt tgatgctaac ggtaagccaa
     1381 ttacctgtaa cgaaaatggc ctgtgcgaaa actcagactt cgactttacc tacacccagc
     1441 ataccgcctg gatttccagc aaaggaacgc tcaccatttt tgataatggc gatgggcgtc
     1501 atctggaaca acctgcctta ccaaccatga aatactcccg ctttgtggaa tataagattg
     1561 atgagaagaa aggcaccgtt cagcaagtgt gggaatacgg taaagaacgt ggctacgatt
     1621 tctatagccc gattacctcc atcattgaat atcaagccga ccgtaacacc atgtttggct
     1681 tcggtggttc tattcatttg ttcgatgtcg ggcagccaac catcggtaag ttgaacgaaa
     1741 tcgattacaa aaccaaagaa gtgaaagtgg aaatcgacgt gctgtcagat aaacccaatc
     1801 agactcacta tcgtgcactg ttagtccgtc cacaacagat gttcaaataa ttaagaaggg
     1861 aataaaaatg tcgaaattag ggatctcatc actatttaaa accatactgc ttaccgcagc
     1921 tttggctgtt tcatttaccg cttctgcatt taccgaaggc acggattaca tggttctgga
     1981 aaaacctatt ccgaatgccg acaaaacgct gattaaggta tttagctacg cctgcccgtt
     2041 ctgttacaag tacgacaaag cggttaccgg tccggtatcg gaaaaagtga aagatatcgt
     2101 tgctttcacg ccgttccatc tggaaaccaa aggtgaatac ggcaaacaag caagcgaagt
     2161 ctttgcggtc ttgatcaata aagataaagc agcaggcatt tccttatttg atgctaactc
     2221 tcagtttaag aaagccaagt ttgcctacta cgcggcttat cacgacaaaa aagaacgctg
     2281 gtctgacggt aaagatcccg ctgcctttat caaaactggt ctggatgccg caggtatgag
     2341 tcaggcggat tttgaagcgg cactgaaaga accagcagtt caagaaacac tggaaaaatg
     2401 gaaagcctct tatgatgtcg ccaaaatcca gggggttcca gcctatgtcg ttaatggtaa
     2461 gtacttgatc tacaccaaga gcatcaaatc catcgacgcg atggcagacc ttatccgtga
     2521 gttagcaagc aaataacagg gaaacaggct atgggtatta agggaatgtg gaaagatctt
     2581 cgcacatcac cagttgacac actggtcaga tggcaggagc aacgcttgtt gtggttgtta
     2641 atggccgttg cgatgggcac gttaattatc ctggcgcact ctttcttcca gatctatctc
     2701 tacatggcac cctgtgagca atgtgtatac attcgctacg ccatgtttgt gatggtgatt
     2761 ggaggattgg ttgcagcgat caatccaaag aacatcatcc tcaagctaat tggctgcgtg
     2821 atggcatttt acggcagcat tttgggactg aagttttcgc tcaaactgaa cgatatccac
     2881 catgcagtac ataatccgga tcccgattca ctgtttggcg tacagggttg ctctaccgat
     2941 cccacttttc cctttaacct gccgctggca cagtgggcac caaattggtt caagcctacc
     3001 ggggattgtg gctatgacgc accaatcgtt cctgatggcg tcacactgag cagcacgcaa
     3061 cagtggtttg tggaaatgta tcagcaatct gaaggctggt atctgctacc accatggcat
     3121 tttatgaata tggctcaggc ttgtatgctg gcttttggta tgtgtctggt gctgctggtc
     3181 attatgagtg gcgcatgggc gcttaaaatc atcagaggct aactcagcaa cacccgaagc
     3241 cagtcattct ggcttcgggt tcttttatta acctatctgc accgacagca tacttaaact
     3301 gcccatttct ataccatcga ccggaatggt aattggctcc tgcccatccc acgcccctaa
     3361 gacatacaac aacggcagat agtgctctgg cgttgggttc gataacgcgc caccttcatg
     3421 gtcgagataa ttcaccagag gatgttgttc caccggccct tgccacgtca gattcgcttt
     3481 cacatactca ttaaacgaca tcgcccacgg atacggtgaa ctatcaccgt gccacttcac
     3541 tgtacgcagg ttatgcacca cgttgccact ggcgaccaac attatccctt catcacgcag
     3601 cgccgcgagt ttgcgcccca tttcgaaatg ccaggcggca ggtttgctac tgtcgatact
     3661 caactgcacc atcgggatat cggcgtcagg atacatctta atcagtacgc cccacgagcc
     3721 gtggtcgaag ccccaggctt ctttatccag cgtcacaggg accggcgcta acagctcaac
     3781 cagatgctgt gccagcgcag gtgaacccgg agcaggataa tgcgtatcgt acagcgcctg
     3841 cgggaagcca ccaaagtcat gaatcgtggg cggcgtctcc atcgcggtca ctcctgttcc
     3901 acgggtaaac caatgagccg aaaccaccac aatcgcttgc gggcgtggca atgtcatccc
     3961 caacttctgc cagctgcggg tatacaaatt atcttccagc acgttcatcg gactaccgtg
     4021 acctaaaaac aatgctggca tacgtgttga agacatgatg atatccttaa ctaaaggtgt
     4081 cattttgata tcctcacaat acgcttgttc ggcggagtaa gaacccggat aacaatgatg
     4141 atgatcatca gttattttga cgatctgcct gaaggtgaag atttataagg agttgtcgat
     4201 gtcagtacct ctcattctga ccatactggc gggggcagcc acgtttattg gcgcgtttct
     4261 cggcgttctc gggcaaaaac cctcgaaccg cttactggcg ttttcgctag gatttgcggc
     4321 ggggatcatg ttgctcatct cattaatgga aatgcttcct gccgcattag ccgccgaagg
     4381 aatgtcacct gtgttgggtt atggaatgtt tatcttcggt ctgcttggct attttggtct
     4441 ggaccgcatg ttgccacatg cgcatccgca ggatttaatg caaaaatcgg tgcagccgtt
     4501 gccaaaatcg atcaagcgca cagccattct gctcactctc ggtatcagtc tgcataactt
     4561 cccggaaggg attgccacct ttgtcacggc gagcagcaac ctggagctgg gatttggtat
     4621 cgccctggcc gtagcgttgc acaatattcc tgaaggtctg gcggttgcag gcccggttta
     4681 cgcggcaacg gggtcaaaac gtaccgcaat tctgtgggcg gggatttccg gactggctga
     4741 aattctcggt ggtgtgctgg catggttaat tcttggcagc atgatttctc cggtggtcat
     4801 ggcggcaatc atggcggcgg ttgccggtat tatggtggcg ctctcggttg atgaattaat
     4861 gccgctcgcc aaagagattg accccaataa taaccccagt tatggcgtgc tatgtggaat
     4921 gtcagtgatg ggattcagtt tagtgctgct acaaacggca ggaattggtt aaagatgata
     4981 atgacagatg ttgcccattg ctaaaggcaa catctgtatc gttattaaag gtattattga
     5041 tgcttttcaa acacttcttt atcggagcgt ttatctaatt tacttcgggc agctaacagc
     5101 gcatctctgt catggcgaaa agctttacag taacg
//
LOCUS       DACADF010000232         5080 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.234, whole genome shotgun sequence.
ACCESSION   DACADF010000232 DACADF010000000
VERSION     DACADF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.234"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..811
                     /locus_tag="ISB30_004574"
     CDS             <1..811
                     /locus_tag="ISB30_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP1992129.1"
                     /translation="NYPTLHQAEKKVADYILSHAHEVVNYSVTELSEKSHASEATIVR
                     TCKKLGYQGYYHLKIALAKEVINPDNSYPENTDFSDITSLATFLLKKQAEDLIQSTQF
                     FNADVLESILKLLANCDTIFFFAAGNSNPLAVYSAYKFSQLGLKTVVHVSPEMQINAA
                     YSMGKRDLAIGISNSGSTNLMVDIFKVVKERGAKSVCITNYIKSPLSKLSTHQLNTAV
                     SDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLGSRFKG"
     gene            complement(841..2838)
                     /gene="tkt"
                     /locus_tag="ISB30_004575"
     CDS             complement(841..2838)
                     /gene="tkt"
                     /locus_tag="ISB30_004575"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005971397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP1992130.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGH
                     DPEAVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAEWDATEKGAQVQQEWDALFADYAKQWPELAAEFTRRMKGDLPA
                     GWVENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(2889..4397)
                     /locus_tag="ISB30_004576"
     CDS             complement(2889..4397)
                     /locus_tag="ISB30_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP1992131.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIISYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWIPVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMTKSGQLVTGDIPVFFAQLRDGAPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            complement(4439..4996)
                     /locus_tag="ISB30_004577"
     CDS             complement(4439..4996)
                     /locus_tag="ISB30_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP1992132.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDTIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALAKKAR
                     QLGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
BASE COUNT         1341 a         1390 c         1150 g         1199 t
ORIGIN      
        1 caattaccct acactgcatc aggcggaaaa aaaggtcgct gactatattc ttagccacgc
       61 acatgaagtc gtgaactata gtgtcactga actatcagaa aaaagccatg ccagtgaagc
      121 gactatcgta cgcacctgta agaaattagg ttatcagggt tattaccatt taaaaattgc
      181 attagcaaaa gaagtcatta atcctgacaa tagctatcct gaaaataccg acttcagcga
      241 tataacatct ttggcaacct ttttattaaa aaaacaggcc gaagatttaa tacaatcaac
      301 gcaatttttt aatgccgacg tgcttgagtc cattctcaaa ctacttgcta attgcgatac
      361 tattttcttt tttgctgccg gaaattcaaa cccactggcc gtttatagtg cttataaatt
      421 cagccaactc ggattaaaaa ccgttgttca cgtcagcccg gaaatgcaaa ttaatgcggc
      481 atattctatg gggaaacgcg acctggcgat tggtatttct aactctggca gcaccaactt
      541 aatggtcgat atctttaaag tggtgaaaga gcgcggtgcc aaatccgttt gtatcactaa
      601 ctatattaaa tctccgttat caaaactctc cacccatcaa ctcaataccg cggtcagcga
      661 taaaatcttc tttgaagcct tcgactccac tcgcgttccg gcaatgggcg ttattgatat
      721 gctggtgctg ttgtttatgt ataaaaacaa ggcgcattac gagaaatacc gcacccgtga
      781 agagtttctt ggctcccgtt tcaaggggta aaaggtcatc ctttcacccc ttgtgcaggt
      841 ttagctgttc agtagctcac gcgcctgcgc caggacattt tccggcgtaa aaccaaagtg
      901 tttgaacaac acattcgccg gagcagactc accaaagctc gtcatgccga tcactttacc
      961 gtccaggccc acgtagcgtt cccagaatcc ggcgatgctg gcttctaccg ccagacgttt
     1021 gcgtacagct tttggcaata ccgactcttt gtacgccgca tcctgattat cgaaacgctc
     1081 ggtacacggc atggaaacca cgcgaacttt acgcccttct tccgttaacg cttttgcggc
     1141 gctgaccact aactcaatct ctgaacccgc agaaatgagg atcatctcag gctgaccgtc
     1201 acaatcactc agtacataac caccacgcgc aatctctgcc aactgtgcag cggtgcgcgg
     1261 ctgttgcgcc agaggttggc gcgttaatac caacgacgtt gggccatctt tgcgctcaat
     1321 cgcctgttgc caggccaccg ccacttccac ctgatcgcaa ccgcgccagg tttccatatt
     1381 cggcgtcaga cgcagcgacg ccagttgctc aacgggctga tgtgtcgggc cgtcttcgcc
     1441 caggccgatg gtatcgtggg tataaacaaa caccgagcgg atcttcatca gcgccgccat
     1501 gcgcagtgca ttacgcgcat attccataaa catcaggaaa gtaccgccgt aagggataaa
     1561 cccaccatgc agcgcaagac cgttcatgat ggccgacata ccaaattcac gcacgccata
     1621 ggagatgtaa ttccccgccg gattttctcc ggtgaaatca accgatttct gatggcgagt
     1681 caggttagag ggcgacaagt ccgccgagcc gcccatcagt tccggcagca tatctgcgaa
     1741 gtgattgagg catttctggc taacctgacg ggttgccagc gccgccggat gggattgcag
     1801 atcgtgcaca tatttctgca tattctcgac ccagccagca ggcaaatcac ccttcatgcg
     1861 acgagtaaac tccgccgcca gttccggcca ctgcttcgcg tagtcggcaa acagtgcatc
     1921 ccattcctgt tgaacctgcg cgcctttctc ggtcgcatcc cattcggcat aaatttcacc
     1981 aggaatttcg aaaggggcat acggccattg cagacgttca cgcaccagcg ccacttcatc
     2041 tgcacccagc gccgagccgt ggcaatcgtg gctgttggct ttatttggcg aaccaaaacc
     2101 gataatcgtt ttgcagcaca gcagactggg cttatccgtg acgcttttcg cttcacgcac
     2161 cgcagcatct accgcttctg gatcgtgacc atcaacgcct tcaatgacat gccagccgta
     2221 ggcacgaaaa cgggcagccg tatcttcagc gaaccagcct tctacgtgac catcgatcga
     2281 aataccgtta tcgtcccaga tagcaatcag attgccgagc ttcagcgttc ctgcaagacc
     2341 acaggcctcg tgggagatcc cttccatcag acagccatca cccaaaaata accatgtgtg
     2401 atgatcaaca atattaaagc ccggtttgtt aaattctgcc gccagcgcct tctccgcaat
     2461 cgccatcccc accgcattag ctacgccctg ccccaacggg ccggtggtgg tttcaacacc
     2521 cggcgtataa ccatattctg gatgacccgg cgtgcgggag tggagctgac gaaagtcgcg
     2581 aatatcatcc atcgacaggt cgtagcctgt aagatgcagc aaagcgtaaa tcaacatgga
     2641 gccgtgaccg ttcgactgaa catagcggtc gcggttaaac cactgcggat tttttgggtt
     2701 atgacgcagg tgacgacgcc agaccacttc ggcaatatcc gccatcccca tcggcgcgcc
     2761 cggatgaccg gagttcgctt tctggatcgc atcaacactt aacattcgga tggcattagc
     2821 cagtaattgt gaattcatac ttcagctctc tctgtctggc gggctgaaac cagcccgcta
     2881 atctcgaatt agttagcaca ggccactgtc atttctgtcg ggcgcggtct gctcattacg
     2941 gttttgattt cgtcgcgcag gatttgcgat ttgccgccaa atatcacctg tacaccgcca
     3001 tcacccagtt tcactaaacc cgcagccccc aactccttca gccgtttttc attcactttc
     3061 ttatcgtcta ccagcgccag ccttaggcgt gttgcgcagg catccacatc ttcgatattg
     3121 tcgaaaccac ctactgcttc gatgatttgg tatggacgtt cttcgcgcgt taatgtggtt
     3181 gcaacggcaa tctcttcatc ctcacgcccc ggtgttttca gattcaactt gcggatcagt
     3241 acccgaaata cggtgtagta aatcgctgca tagcccgcgc ctgccaggaa caccagatac
     3301 cacggcgtgc ggtttggcat aacgccattc agtacgaaat caataagacc gccagcaaaa
     3361 ggatgaccaa tgtgcacatt caggatgttc atcagcacaa atgaagtggc atataagaag
     3421 atgtgaatga tgtagagaac cggcgctgca aacaaaaatg catattccag cggctccgta
     3481 atcccggtaa caaatgtggt cagcgcaccg gagagtaaaa ggcctttaat cagcgcctta
     3541 cgttccggtt ttgcttcctg atacatcgcc aacgcggcgg caggtaacgc aaacatcatg
     3601 atcgggaaac gacctgtcat aaacagcccc gccgtcaacg gagcaccgtc gcgcaattgg
     3661 gcaaagaaca ccggaatatc gcccgtcacc agttgcccgg atttggtcat gtagtcgcca
     3721 aagttgtaat aaaacggcac gttccagata tgatgcaggc caaaaggaat cagcgaacgt
     3781 tcgccaacgg cgtaaataaa ggccgcgaag ttcgcgttat gtccactggt caccagcgtt
     3841 gaaagcgacg ccagaccttt ttgtaccggt atccacagcc acggcaataa atagccgaca
     3901 ataattgccg ccacagccgt aataatcggt acaaagcgtt ttccaccgaa gaaaccaaga
     3961 aactgaggca attcaatatt gtggaagcgg gtatagagca gataaacaaa gatggcgaca
     4021 atcagaccgc caaacactcc cgtttgtaac gtcggtaccc cgaggatcat cgcataatca
     4081 ccgttactgg ctgcaacgga ctccgcagta atgccaagtt gcgttcccat cgtggcattc
     4141 atgatcatgt aactgataat cgctgaaagt gatgaagaac catcacctgt caggccaatc
     4201 acaataccga cacaaaacag caaaggaagg ttggcgaaaa tcaggccgcc gcaattcgcc
     4261 agcagctcaa tgttaaatga aatcccgatg gctaaaatca aacccgccgc cggaagaatg
     4321 gcgattggta acatcagagc agaagaaaat ttctgcaact tcttgaagtt aaacaaggat
     4381 gttatttttt tgctcatttt tattctcctg tagggtatcg agcaaataac ggcgacgact
     4441 attccagatt ggcgtggcgc ttaaacagtt cactttccgg cgcaccactt tcagcagcca
     4501 tcttcatcac taatgcatcg gcaatcacca gcaaagcttg ctcatacaac gacgccatcg
     4561 gctgtgccga gcggaaaaca gaatcttttt gatttttgga aggggcattg atttgcacga
     4621 tgacgtcgac aaactcggtg atagtggcgt gtcgattcgt ggttaacacc gccacagcgc
     4681 cgccgagctg acgtgctttt ttcgccagcg ccaccagttg tgctgtctca ccggaagcag
     4741 aagaaacgat aaacagatcg ccaggcccaa agttcggcgt aatggtttcc gtcaggaagt
     4801 aaacctcttt tcccatatgc atcaggcgca tggtgaaggc tttcatagaa aaaccggcgc
     4861 gtcccaggcc atagcagaaa acgcgctggc tttgctgaat agtgtcgatc aatttggtgc
     4921 agtcatcctc acggatagag tccttaacga aggttaattc attgatgatc tcagtgatgt
     4981 acagctttgc gtccatttca cacctcatga aataattttc tcaaacacaa aatctcatga
     5041 aattttattt cattccaatg catcagtatg aaatgtgaag
//
LOCUS       DACADF010000233         4992 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.235, whole genome shotgun sequence.
ACCESSION   DACADF010000233 DACADF010000000
VERSION     DACADF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.235"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            124..345
                     /locus_tag="ISB30_004578"
     CDS             124..345
                     /locus_tag="ISB30_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992133.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            776..1801
                     /locus_tag="ISB30_004579"
     CDS             776..1801
                     /locus_tag="ISB30_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP1992134.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPTEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1869..3050
                     /locus_tag="ISB30_004580"
     CDS             1869..3050
                     /locus_tag="ISB30_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP1992135.1"
                     /translation="MPHRRSTVKGSLSFANPTVRAWLFQILAVVAAVSIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRIFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     FAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSIYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            3060..4163
                     /locus_tag="ISB30_004581"
     CDS             3060..4163
                     /locus_tag="ISB30_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP1992136.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLVVWWLMYGGFLGLDRVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            4171..4929
                     /locus_tag="ISB30_004582"
     CDS             4171..4929
                     /locus_tag="ISB30_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP1992137.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
BASE COUNT         1168 a         1160 c         1270 g         1394 t
ORIGIN      
        1 attcacataa tttgtcatta ttcattccgc aagatttata acctccagtg ataaaatggc
       61 attcagctcg ttaataagag agttaactta ttaagcgtta gcgtttatta ctgaggtaac
      121 accatgaaaa gattaattcc tgtggcattg ctcaccgcat tgctggcggg ctgtgctcac
      181 gattcgccct gcgttccggt ttatgacgat cagggtcgac tggttcacac taatacctgt
      241 atgaaaggca cgactcagga taactgggaa acggctgggg ctattgccgg cggggcagca
      301 gctgttgctg gcctgacgat ggggatcatc gctctgtcga aataactaaa cagggcgcgg
      361 agctccgcgc tctttttaac gatacagtat ctttccataa gataaaatat tgctatattc
      421 tgctgggtaa ttccctgctg atttagcatg ttatatcacg ttatttcgct gctttcgaaa
      481 taacgcatca atttaacttt cacacttccc accagaaata tactcacgct aacaatcatt
      541 attctgctta gtaaaaaacg cgtaaatatt ttgcgccaaa atgtggcgca tgtttcattt
      601 tcgcaccatt acgaggcgct gtttttatta tttcctgtct acactcagct tattgcgacg
      661 cgaaatcgtg cgcaaaagct ggcactactt ttgcttataa gaggatggcc acagacaggt
      721 aaaagacgtt tcccaaacgt cctataacga taattttcgc ctcacaggaa gcattatgaa
      781 aaagatgatg atagccacac tggctgccgc cagcgtgctg cttgccgttg caaatcaggc
      841 gcatgctggc gcgacgcttg atgccgttca gaaaaaaggt tttgtgcaat gcgggatcag
      901 tgatggatta cctgggttct cttatgccga tgctgacggt aagttttcag gtattgatgt
      961 agatgtttgt cgtggtgttg ccgctgctgt attcggtgac gatacgaaag tgaaatatac
     1021 cccgctcact gcaaaagaac gcttcaccgc tttacagtca ggggaggtgg atttgctctc
     1081 ccgtaatacg acctggactt catctcgcga tgccgggatg ggaatggcat ttactggcgt
     1141 cacttattac gacggcattg gcttcctgac gcacgataaa gcggggctaa aaagcgcgaa
     1201 agaactggat ggcgctaccg tctgtattca ggcggggact gataccgaac tcaacgtcgc
     1261 cgattacttc aaggcaaata atatgaagta cacgccagta accttcgatc gctcagacga
     1321 atcagcgaag gctctggaat ctggccgctg tgatactctg gcctcggatc aatcacaact
     1381 gtatgcccta cgcatcaaat taagcaaccc aactgaatgg attgtcttac cggaagttat
     1441 ctctaaagaa ccacttgggc cggtagttcg tcgtggcgat gatgaatggt tctcgattgt
     1501 acgctggacg cttttcgcca tgctgaatgc tgaagagatg ggcatcaatt cccagaatgt
     1561 cgatgaaaaa gcggctaatc cagcaacgcc tgatatggca catctgctgg gtaaagaagg
     1621 cgattacggc aaggatctga agctggataa taaatgggcc tataacatca tcaaacaggt
     1681 gggtaactac tcggaaattt tcgagcgtaa cgtaggttca gaaagcccgc tgaaaattaa
     1741 acgtgggcaa aataatctct ggaataacgg cggtattcag tacgcgccgc ccgtgcgtta
     1801 agtcggtgtg aggtaacgag cgttgctacg gcggcgctct tttcagcgtt atggttttcg
     1861 aggtttctat gccccatcgc cgctcaaccg ttaaaggctc actctctttt gccaacccta
     1921 cggttcgcgc ctggttattc cagatccttg ccgttgttgc tgctgtcagc attgttggtt
     1981 ggttatttca caacactgta acgaatctca gtaatcgtgg cataacatca ggatttgctt
     2041 ttcttgaccg cggggccggt tttggcatcg ttcagcattt gattgattat cagcaaggcg
     2101 acacttacgg acgcattttt attgtcggcc tactcaatac gctactggtt tctgcattgt
     2161 gtattgtgtt cgcttctgtt ttgggcttct ttatcggtct ggcgagactt tcggataact
     2221 ggctgctacg aaagctttcc acaatttata ttgagatctt ccgtaatatt cccccgctgc
     2281 tgcaaatctt cttctggtac tttgccgtgt tgcgcaattt gcccggacca cgacaggcag
     2341 tgagcgcgtt tgacctggcc tttttgagca atcgtgggct ttatattccg tcgccgcagc
     2401 tgggagacgg atttattgcg tttatcctgg ctgttgttat ggctatagtc ctttctgttg
     2461 ggctattccg ctttaataaa acataccaga taaagaccgg acaactgcgc cgcacctggc
     2521 cattcgccgc tgtgttgatc attggtttgc ctttactggc gcaatggctt tttggcgcag
     2581 cactacactg ggatgtccca gcccttcgag gctttaattt ccgcggcggg atggttttaa
     2641 ttcctgaact ggcagcctta acgctagcac tttcgattta tacctctgca tttatcgccg
     2701 agattatccg cgcagggatc caggcagtgc cttatggtca acatgaagcg gctcgatcac
     2761 tgggattacc caacccggtt acgctacgcc aggtcattat tccccaggca ttgcgggtga
     2821 ttattccacc gttaaccagc cagtatctca acatcgtcaa gaactcctct cttgccgccg
     2881 ctattggcta tcccgatatg gtttcgctgt ttgctggcac cgtgctgaac caaacggggc
     2941 aagccatcga gacgatagcc atgaccatgt cggtctatct gattatcagc ctgactatct
     3001 cgctgctgat gaatatctat aaccgccgca tcgcgatcgt tgaacgctaa ggagtcatga
     3061 tgacaaaagt attgctgtct catcccccgc gcccggcgag ccataactca agccgcgcga
     3121 tggtgtgggt gcgaaaaaat ctgttctcca gctggagcaa tagcctgctg actattggct
     3181 gcatatggtt gatgtgggaa ctgatcccac cgttgctgaa ctgggcattt ttgcaggcta
     3241 actgggttgg ctcaacgcgt gccgactgca caaaagccgg tgcctgttgg gtcttcatcc
     3301 acgaacgatt tggtcagttt atgtatggac tttacccaca cgaccaacgc tggcgaatta
     3361 acctggcatt actgattggc cttgtgtcta tcgcaccaat gttctggaaa atacttccgc
     3421 atcgcggtcg ctatattgcg gtctgggcgg tgatttaccc actggttgtc tggtggctga
     3481 tgtatggtgg gtttcttggt cttgatcggg ttgaaacccg gcaatggggc gggttgacgc
     3541 taactttaat tattgcatca gttgggattg cgggggcgct accgtggggg atcttactgg
     3601 cgttaggtcg ccgctcccat atgccgattg tgcgtatctt atcggtcatt tttatcgagt
     3661 tctggcgcgg cgtaccgctg attaccgtat tgtttatgtc ttcggtcatg ctgccgttgt
     3721 ttatggcaga aggcaccagt atcgataaac tgatccgcgc gctggttggc gtgatcctgt
     3781 ttcagtcagc atatgttgcg gaagtcgtgc gaggcggatt acaggcgctg cctaaagggc
     3841 aatatgaagc ggcagagtcg ctggcattgg gttattggaa aactcagggg cttgttattc
     3901 tgccccaggc gttgaagctg gtaattcctg ggctggtaaa taccatcatc gcactcttca
     3961 aagataccag tctggtgatc atcatcgggt tgttcgatct tttcagtagc gttcagcagg
     4021 caaccgttga tcccgcgtgg ttgggtatgt cgacggaagg gtatgttttc gccgcgctga
     4081 tctactggat cttctgtttc agcatgtcac gttatagcca acatctggaa aaacgtttta
     4141 acaccgggcg tacaccgcat tgaggacact atgagccaaa ttttactgca acctgctaac
     4201 gcgatgatta cgctggaaaa cgtcaataaa tggtatggac aattccatgt tttgaaaaat
     4261 ataaatttaa ccgtgcaacc gggcgaacgg atcgttctat gtggcccttc aggttccggt
     4321 aaatcaacaa ccattcgttg tattaatcat ctggaagaac atcaacaggg acggatcgtg
     4381 gtagatggca tcgaacttaa tgaagatatc cgcaatattg agcgcgtcag gcaggaagtg
     4441 ggaatggtct ttcagcattt caatctcttc cctcatctga ccgtgttaca gaactgtacc
     4501 ctggcaccga tttgggtacg caagatgcca aagaaagagg ctgaagctct ggcgatgcat
     4561 taccttgagc gggtgagaat tgccgaacat gcccagaagt ttcccggaca gatttctggt
     4621 ggtcagcagc aacgtgttgc catagcacgt tcgctgtgta tgaagccgaa aattatgttg
     4681 tttgatgagc caacgtcggc gctcgatcct gagatggtga aagaggtgct ggatacgatg
     4741 attgggctgg cgcagtcggg tatgacaatg ttgtgtgtaa cacatgagat ggggtttgca
     4801 cgaaccgtcg ctgaccgggt gatttttatg gatcgtgggg aaatagtgga gcaagctgca
     4861 cctgatgaat tttttgcgca tcctaaatca gagcgtacga gggcattttt atcgcaggta
     4921 atccattaat tgaatattag ttcgaaaagc aaaaaggcca tcctttcgga tggcctttcg
     4981 cctgatttga tg
//
LOCUS       DACADF010000234         4955 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.236, whole genome shotgun sequence.
ACCESSION   DACADF010000234 DACADF010000000
VERSION     DACADF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.236"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..83)
                     /locus_tag="ISB30_004583"
     CDS             complement(<1..83)
                     /locus_tag="ISB30_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1992138.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADA"
     gene            246..500
                     /locus_tag="ISB30_004584"
     CDS             246..500
                     /locus_tag="ISB30_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP1992139.1"
                     /translation="MKNGIDVSVIIPAYNAAETSVSLVTKILSEPHLAIELIIVDDGS
                     TDETGNVLRQIHDDRLILIEQANQGVYAARNAALALHQGE"
     gene            1604..3679
                     /locus_tag="ISB30_004585"
     CDS             1604..3679
                     /locus_tag="ISB30_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP1992140.1"
                     /translation="MDLVDSVEAGKLTISELIDALAKDKNYSASKWDQRYREFTTLLQ
                     QTSTFSEPETDGLVKRLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYRSLRNRVFAAFSPENYTSTVDENAFSKAAEFLNQH
                     FHLGLALTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKIA
                     STTFITSNEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWLLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLSQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPANVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRHHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            3672..>4955
                     /locus_tag="ISB30_004586"
     CDS             3672..>4955
                     /locus_tag="ISB30_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HAP1992141.1"
                     /translation="MGEVISVFEYDLLGSDKAASVGAKLVPPLVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIETQQATLQAQRMPLLEIFIHQFLHSVSQLLKQGLRSDYVSEQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLKLSSENQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPYELPSHLKVIPQAATYSLVKHGSNDCFKLRPDLLIQSRRPVQTK
                     LVMDTKWKLINSNSQKTTQYGLAQADFYQMFAYGQKYLNGIGEMYLIYPAHDQFNQPI
                     QQHFAFSKTLKLWVVPYQITANRGERMMWVKDVSSI"
BASE COUNT         1430 a         1131 c         1090 g         1304 t
ORIGIN      
        1 gcatctgcca cggtgtaatt ctggtcaacg accagttgag cggattcgcg tttaaactct
       61 gcgctgaaat ttcttttttt cattatggca cctgtgttgt tctgaggtga gcatatcacc
      121 tctgttcagg tggccaaatt cagtaaacca cttcattatt cgatatcgat gaggaaatca
      181 gggaaagaag gattatcacg atcagacgcc tcaaagatga caacgaaaga aaatcgtagc
      241 tcaatatgaa aaatggaatc gatgtcagcg tcattatccc tgcttacaac gccgctgaaa
      301 ccagcgttag tttggttact aaaatcctca gtgaacccca cttggctatt gaacttatca
      361 ttgtcgatga cggttctact gatgaaactg gcaacgttct tcgtcaaatc cacgatgatc
      421 ggctcattct cattgagcag gccaatcaag gtgtctatgc cgcccgaaat gctgctctag
      481 ccttgcacca aggagaatag gttatattcc ttgatgcgga cgacgatata gcagatgggt
      541 tcatagaaaa aagatggcag gcagttagta cgtcgcaagc cgacgtcatg atctttaatg
      601 gctggcattc ctgtgctgac caacatcgaa gagcggttca caaaaagcag ccctatggta
      661 cgcctctgtc aggttattaa tggattagac actgtgttac acaccgcgaa tggccgcatt
      721 atctgtggct gcagatggtc aggccatcct atgtcaggca gcattcactg cgcttccagt
      781 ccggcaggag ccataaagac attttttaaa cggttcatct tgcagcgagc aatggttgat
      841 tctgtacatt ggatataaag gactatatct atatcaacaa taaggcctct atcacacatc
      901 ggccggatta ctacgttgtt cgtgctataa actatgttga agtgatcgcg aaaatcatcc
      961 aagtagcgga acaagaacaa aacaaaaaat aaaggtattt ttgtaccggc atgcgctggt
     1021 agaggcacgt cattttttgg gactctaccg caatcgcgtt ggggataaat cgggcatgaa
     1081 ggactgcttc aaagcgaaca tattcgcagc ccatttattc agaggtatca gcagttttag
     1141 cgatgtgttt ttctttatta agctgctggg caagctattc tgataagttg tgcgttgcta
     1201 cacgacacca gcgcacagct taatttcacg ctttctgact aaattgttca ggccttttct
     1261 ataggtgcaa acacaactcc ccgtactttc ctcttccaac tcaaaatcat cgcaccttgc
     1321 tgacccaaga agggagcata ccctgcacta gtatccgcac agctaatact ctattgatac
     1381 caacaacgct tcagcacaac aaagtaactc gataatcaca actcaaagtg aagatgccaa
     1441 aaactcgttt gagagcaata aattcaataa catgaaaaaa atgtattttg cattgaaaat
     1501 cttacttatt gcgcttggct acaattgcat gcttggtaat atagattata aaacaccaga
     1561 ctagctgcct ggtaagtaat gtaatagcag aggaaatatc acgatggatc ttgtagatag
     1621 tgtcgaagca ggtaagctga cgatcagtga attgattgat gccctggcga aagataaaaa
     1681 ttactcagct tccaagtggg atcagcgata ccgtgaattt acgaccttgc tacaacaaac
     1741 ctcaactttt tctgaacctg aaacggatgg tttagtcaag aggctctggt atgaacgcga
     1801 taacggtatt gccagtattc gccagggcgt tccatcctta gcagaatatc agcaaagcct
     1861 gccactgctt agagaactaa ctgaacgaat tcggcaacag ccggatgaag aaacttacca
     1921 atatgttggc aatgcactac aacaagctaa agaaaacgga cttctcaagc gtatgtatag
     1981 gagtttgaga aatcgcgtct ttgccgcgtt ctcgccagaa aactacacca gtactgtgga
     2041 tgagaatgct tttagtaaag cagcagaatt cctaaatcag cacttccatc tcggtctggc
     2101 actgaccgga aattggttac agaaaaacta tgaattgaaa caagccatac acgcccaatc
     2161 tcctgacaca gatccttatt acgtgaatat ggccatctgg catctctatg aattgctccg
     2221 tgaacgcgat aatgaacaaa agcaggagaa aatagcgagc actacattca taaccagcaa
     2281 tgagcccatc gagaacaaga tcatcctaca ttcaccaact aacgtgatct tctttggccc
     2341 ccctggcact ggcaaaacct tcaggttgca gcaaaaaatg aaagagtaca cttctcatgc
     2401 tgttccagct gatcgtgatg cctggctgga ttctcgcctt gaatcgttga attggatgca
     2461 ggttataacg ctggtgctgc tcgatcttgg gaaacgagcc aaagttcgcc aaattattga
     2521 acatatgtgg tttcaacgta aggcattatt aaacggtcgt aatggcaacc tatcgaatac
     2581 agcctgggca gctttgcaat cctatacagt tcccgagtcg ttaaccgttg attataagaa
     2641 tcggcgtgag cctgccgtat ttaacaaaac agataacagc gaatggttgc tagttgattc
     2701 acagctcgag caagtggagg atttggtaga gctctacgcc gaacttaaac gaggccctaa
     2761 atctgccgaa gccatccagc gttttgcggt ggttacgttc caccaatctt acggctatga
     2821 agaatttatt gaaggtatac gcgctcgctc tgacgagagt ggcaatatct cttatcccat
     2881 tgagccgggt atctttatgc gcctttccca acgtgccaat gccgatccag gacatcgcta
     2941 cgccattttc attgatgaga tcaatcgcgg taacatatcc aagatctttg gtgaactaat
     3001 ctcactcatt gaagtagaca agcgtgcagg catgcccaat gcaatgagcc tacaactggc
     3061 ttatagcggt gatcacttca gcgtacccgc caatgtcgat atcatcggag ccatgaatac
     3121 agcggaccgt tctttagctc tgatggacac ggctttgcgc cgtcgctttg actttgtcga
     3181 aatgatgccc gatctctctt tactgagtgg agctaaggtg aaaggcatag agctcgagtc
     3241 gttgttagag aaactcaata gccgcatcga ggctctttac gatcgtgaac atacgctggg
     3301 gcatgcgttc tttatgccgg taaaaaatgc actcgatgcc ggtgatgaag aagctgcgtt
     3361 taaacaattg aagatcgcat tccagaaaaa gatcattccg cttttacagg aatacttttt
     3421 cgatgactgg aacaagatcc ggttggtgct ggcagacaat caaaagcaag acgacaacct
     3481 gcaattcgtg attgagaaaa ccgacgatct cgatacgctt tttggtaaca accatggttt
     3541 acgacaccat gatcagcaat caacagctta cgaactcaaa gatttcgatc aagagatctg
     3601 gaatattcca caggcttatc gttcaattta tcagccccag cagactcccc ttgatgagca
     3661 ggctgtaaat catgggtgaa gttatctccg tttttgaata tgacctattg gggagtgaca
     3721 aagcggcttc agttggcgca aaactggtgc ctccactggt ctttaattat ttagaagcgc
     3781 ttagcttggc aagtaatcaa gggagccagt tcctcaaact cacctctcgc tcaggcttca
     3841 aattgcttca ggttcaaaac tacgcgggga tgctttcaac acctcacggt ttccagcttg
     3901 agatactgcc caaggttggt aaaaacctca cagcggctaa tgcacgtcaa acattactga
     3961 cgatgctaag ccatttgcct gggtttcgac atattgaaac tcagcaagcc actcttcagg
     4021 ctcagcgtat gcctttgctt gagattttta ttcaccagtt tttgcatagc gttagccaat
     4081 tacttaaaca aggtttacgc tccgactatg tgagcgagca aggtaatctg gcttttatga
     4141 agggcaagct gatgctttct gcacagctgc gacataacgc ggtgaatcgc cataagtttt
     4201 gtgtcgatta tgatgactac atgcctgatt gtgcagccaa tcggctctta cactccgcgc
     4261 tagataagct tcttagtcta aagctgtcat cagagaatca acgctggctt tacgaactgc
     4321 gctttgcgtt tgacggtatt ccacttagcc gggatattga acgtgatata aacaacttac
     4381 gcctcgagcg tggtatggct cattacaacg agccaatggc gtgggcgcaa ttgatcctga
     4441 gaggaatgag tccgagtgct ttgcagggaa acaccaaagc gatatcactt ttgttcccta
     4501 tggaagcggt atttgaatcc tttgtggcac agaccctacc ctacgaatta ccttctcatc
     4561 tcaaagtaat accacaagcg gctacttatt ctcttgtgaa gcacgggtct aacgattgct
     4621 ttaaacttcg gccggatttg ttgattcagt cacgtcgacc ggtacaaacc aaattggtga
     4681 tggacacaaa atggaagcta ataaatagca actcccaaaa aacaacacag tatgggctag
     4741 cgcaagccga cttctatcaa atgtttgcct acggtcaaaa atacctaaat gggattggcg
     4801 aaatgtactt aatctatcct gcgcatgatc aatttaacca accgatacag cagcattttg
     4861 ctttctctaa aacattaaaa ctatgggttg ttccgtacca gataacagca aaccgcgggg
     4921 agaggatgat gtgggtgaag gatgtgtcca gtata
//
LOCUS       DACADF010000235         4812 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.237, whole genome shotgun sequence.
ACCESSION   DACADF010000235 DACADF010000000
VERSION     DACADF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.237"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..848
                     /locus_tag="ISB30_004587"
     CDS             <1..848
                     /locus_tag="ISB30_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP1992142.1"
                     /translation="AQQLLTLANETLEKQVKERTSALELINQKLIQEIKERSQAEQVL
                     QITRSELAESSKLAALGQMATEIAHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNLK
                     HIISVIERMTQLISELKAFASRHRVPKGSADVIKVMYSAVALLNHSMEKNNIERRIKA
                     PSMPLFVNCDELGLEQIFSNLISNALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGG
                     FAPEVVDRIFEPFFTTKRRGMGLGLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEW
                     GEKHE"
     gene            841..2199
                     /locus_tag="ISB30_004588"
     CDS             841..2199
                     /locus_tag="ISB30_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAP1992143.1"
                     /translation="MNNKFSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(2188..3261)
                     /locus_tag="ISB30_004589"
     CDS             complement(2188..3261)
                     /locus_tag="ISB30_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HAP1992144.1"
                     /translation="MSLQIAVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     TKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(3273..4772)
                     /locus_tag="ISB30_004590"
     CDS             complement(3273..4772)
                     /locus_tag="ISB30_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HAP1992145.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSSAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSVILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     SWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
BASE COUNT         1365 a         1172 c         1142 g         1133 t
ORIGIN      
        1 atgcacaaca attattaaca ctggcgaatg aaacattaga aaaacaggtt aaagagcgta
       61 catctgccct ggaattgatc aatcaaaaat taatacagga gataaaagag cgcagtcagg
      121 ctgaacaagt attacaaatt acgcgtagtg aactggcaga gtccagcaaa ctggcggcgc
      181 ttggacagat ggcaaccgaa attgcccatg aacaaaatca accgttagcc gccattcacg
      241 cacttactga taacgcgcgt actatgctaa aaaaagagat gtatccacag gttgaacaga
      301 atctgaaaca cattatttcc gtgattgagc ggatgacgca gctcatttcc gaacttaaag
      361 catttgcctc gcgccatcgc gtacctaaag gttctgccga tgtcatcaaa gtgatgtata
      421 gcgccgtggc gttacttaat cacagcatgg agaaaaataa cattgagcga cgaataaaag
      481 ccccatccat gccgttattt gtcaattgcg atgaactcgg tcttgaacag atattcagta
      541 atttaattag caacgcctta gattctatgg aaggtagctc ttacaaacga ctggatatcg
      601 ccattcgcca ggcaaataac aaagttatta ttaccattaa agacagcggt ggcggttttg
      661 cacctgaagt tgtcgatcgc atatttgaac cattttttac cactaaacgt agaggaatgg
      721 ggttgggact ggcaatagtc agcgaaattg tccgaaattc gaacggcgca ctccacgcca
      781 gtaatcatcc tgaaggcggc gcagtaatga cattaacctg gcctgaatgg ggagaaaaac
      841 atgaataata aattcagtga aattactata gtttatattg aggatagtga cgatgtacgt
      901 tttgcctgtg aacaaacgct cacgctggct ggttatcgcg ttatttcctg ttgcgatgca
      961 gaacacagta ttccccttat tcaaagtcag gccaacatta ttatactgac cgatgtcagg
     1021 ttacctggaa tatcaggact agagctcctc agttacatca acgaaatgga tagcaaaatt
     1081 ccggttattt taattaccgg acacggtgac gtggaaatgg cggtggatgc catgcgtaat
     1141 ggtgcattcg atttcattga gaaaccaagt agctcagata aattattgag tattattgcc
     1201 agagcggtag aaaaacggcg attggtttta gaaaatcagc aattactggc gaatctgcaa
     1261 caggaaaatg gcccggtgct gattggtcgt agtccgcaga tgcagcaact gcgtaaaatg
     1321 atccttaatg tcgcagatac cggtgccgat gtccttattt atggtgaaac aggttgtggt
     1381 aaagaagtcg tcgccagaat gctgcatcac tggagtaccc gccgtcaggg gcagtttgtt
     1441 gccttaaact gtgcaggctt accagaaacg ttgtttgaaa gcgaaatatt tggtcatgag
     1501 gcgggcgcat ttaccggtgc ggttaaaaag cgtattggaa aaattgaaca tgcaaatggt
     1561 ggtacgttat ttctcgatga aatagaggga atgccatcag gaatgcaggt aaaactgcta
     1621 cgtgtattgc aggaaagaac cattgaacga ttaggtgcca atcaattaat tccggtgaat
     1681 tgccgtgtta ttgcagcgac caaggaagat ttactgcgtc gcagcgaaga acatctgttt
     1741 cgtctcgatc tctattatcg tctgaatgtg gttagcctga atattccacc tctacgccag
     1801 cgacgagaag atatccctga actgttttac tggttcgcca gtcaggcggc acaaaaatat
     1861 aatcgtcctc tgcctgatat ttctcccatg ttactggcct ggttgcagtc acaaagttgg
     1921 cctggcaatg tacgtgaact taaacacaat gcagagcgtt ttgttctcgg tttactgacc
     1981 caccaccagc cagtgccaat gactcagcaa gaagagagtg gtcttaccgc ctgcattgat
     2041 gctttcgaga aaaagttaat cgaagatatg ctgcgacaaa cggaaggcca ggtcagtttg
     2101 acagcgcggc tattacagct gccgcgcaag actttatatg acaaactcaa caaacatcag
     2161 attcaaccgc aagtttatcg accagaatca tccagctaat gctgattcct gaggatgtaa
     2221 ccccaaatcg atacctttgc gggcgtaatg ttcaactacc caggaccaga atgataccgg
     2281 gatcgtaatc atcttcgcat tgcgttcccg gttggcagct tctggcatac cggggtaaat
     2341 caccggattg ccgccaggtt gaggctgcgt agctaacaga taatccgcaa agaactgccc
     2401 tttctcccct gccagatcgg tgaattgatc aggatcgata atcaacgcaa aaagatgatt
     2461 atgcgcagat ccatccgtct gatgatcttc ggcgatcgtg tcaccaccag ataagatccc
     2521 cgccatcatt tcaatcatca gtgccaggcc aaatccttta tgctcaccaa atggcagtaa
     2581 cgcccctgga ggattgctga ataacacccc aggatcattg cttggattcc cctgtgcatc
     2641 aatcaattgt cccggcgcgg cctgcttccc ggtggacgac attacccgtg ctttatttag
     2701 cgccatgcta ctggtggcaa aatcaaccag aatcggcgga cgcccatcac tgaacggcca
     2761 ggcaaagcag aaagggttag tggtcatacg cggttccgca ccgccccacg gggcgaccag
     2821 tgcaggacgc ccggtgacgt tactcatggc cagcatcgcc aggccttgat cagcaaccat
     2881 ttgcccgtaa tgccccatac gccctaaatg atgcgcatgg cacaacgtta acaggcaaaa
     2941 accgcggtgt tttgtttgtt cgatggcagc atttaccgcc acttttccgg cgtgttgacc
     3001 aaagccattg tgggcatgaa aacgctgtaa attaccttca cttgttaagc attccgggcg
     3061 ggcattcgcg gttacatcgc cacgagcgat attttccaga tatttcggta gcagcgtgac
     3121 gccgtggctg gcaaagccat aacgttccgc ttcaatcata tgttccgcca catcctgagc
     3181 gatatcggca tcacagccat taagttccag cagcccggca gctaaccaac gcactttttc
     3241 aacagcaatt tgtaatgaca tcgttggttt cctcacatta gacctaaaat tttccaccag
     3301 gtggcggcaa acagtagcaa caacaggtag ccgattaccg tcaggtacag tcccacacgg
     3361 gtaaactggc gtggcgtgaa ggtatccgta cccatacaca ccatattttg tggcgcgtta
     3421 atcggcagga tgaagccaaa actgacactg aaggcgagca gaatggtcat gccgaccggg
     3481 ttaacgccga gttctggcgg cagactgctt aacaggctaa tcaggatggg cagcaatgcg
     3541 gcagttagcg cagtggcact ggcaaacccc aggtgaatga ttatcaagaa agcggcgaga
     3601 atcgcgaaga tggcaagtga tggtaaaccg tcaagaccaa aacctttcac cacatagttc
     3661 gccatccatg aagctgcctg tgtatcaagc aacgtggacc ccaggctgat accaatacca
     3721 aacatcagca acgtgcccca ctgcacacgt ttttcgacct ctttccagct cattacaccg
     3781 atgcctggca gcaacataat cgccagtccg gcaagggtga cagaggtggt atcaatactg
     3841 tgtaattttc cgccagtaga ccaaaagagc agtaacaaca gcgaaatacc gatcaagcgt
     3901 ttttctttac cggtggttgg ccctaactct gcgagtgctt ttttaatggc ctcgctcccc
     3961 ccttcaacag cttccgtttc tggcggcaac aatttacgtg cgaggaaata gaggatcacc
     4021 gacatggtga ggctccaggg cgcgcccgct aacagccagt caagccagct gacagaatgc
     4081 ccggcgccga aagttttatt gataaaaccg atagaaagca ggttttgcgc cgacgaagtt
     4141 tgaataccaa cgttccagat actggtcgcc tgggcgataa caatcatcat tgacgccgct
     4201 aaacgtgaat gtttatccac cttaaatgcc gcaatgaccc ccatcataat cggcaccacg
     4261 caagccgtac gggcagtggc actgggtacg accaggctca ggacgatggt caccacaatg
     4321 gcaccgataa taatgctgcg gctactggca cctattttcg acatagtaaa cagcgcaata
     4381 cgtttatcca gcccggtaat ggtcattgcc gccgcgataa acattgctgc tgccaccagc
     4441 gccagagcag aattagaaaa acccgacaga gtcattttca gcgctttcgc ggtgccaagg
     4501 atcgtgtccg ggtgattcat atccggggca aaaccaacca taaagataat cagtgcggaa
     4561 ataacaattg cactggcggt gtaatccatc gcttcgctga tccagacaat aatggcaaac
     4621 accagaatgg cgatcatatt ttgtccggcg acgggcaagg tatcgcccag aggcagaaga
     4681 gtaataataa ttccggcaat gatcgccaac cacaggccgg gattagggat aattcgacgc
     4741 ttttgtggtg cgccatgtga tattgatgac atattcacct cattgtgaaa aataaaaaac
     4801 ctgcctggct at
//
LOCUS       DACADF010000236         4809 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.238, whole genome shotgun sequence.
ACCESSION   DACADF010000236 DACADF010000000
VERSION     DACADF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.238"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            129..1796
                     /locus_tag="ISB30_004591"
     CDS             129..1796
                     /locus_tag="ISB30_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HAP1992146.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEKALKQLAQGSGSSQAL
                     TQVRRWDSASQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            1806..3065
                     /locus_tag="ISB30_004592"
     CDS             1806..3065
                     /locus_tag="ISB30_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAP1992147.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIACLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            3076..3891
                     /locus_tag="ISB30_004593"
     CDS             3076..3891
                     /locus_tag="ISB30_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAP1992148.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            3888..4781
                     /locus_tag="ISB30_004594"
     CDS             3888..4781
                     /locus_tag="ISB30_004594"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP1992149.1"
                     /translation="MKTLVVALGGNALLQRGEALTAKNQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
BASE COUNT         1035 a         1220 c         1479 g         1075 t
ORIGIN      
        1 ttgaattctt ttgttaataa tccccgtgtg atattcatca ccttatttac tcgttgtcat
       61 cgataccgta atcgccacat taacactgct cgtgcaattg ccatgggtgc aatttttaag
      121 gagttgttat gatccacgcc tttattaaaa aagggtgttt tcaggattcg gtcagtttaa
      181 tgattatttc acgaaaactc agcgaatcag aaaatgttga tgatgtttcc gtaatgatgg
      241 gtacgcccgc caataaagcg ttattagata ctacaggttt ctggcatgac gattttaata
      301 acgccacgcc gaacgatatt tgcgtggcaa ttcgtagcga agcggcggat gcggggatcg
      361 cgcaggcggt tatgcagcag cttgaaaagg cgctaaaaca actggcgcag ggttcaggca
      421 gcagccaggc gttgacgcag gtgcgtcgct gggacagtgc cagtcagaaa ttacccgatg
      481 ccaatctggc gctgatttca gtggctggtg agtatgcggc ggagttggcg aaccaggcgc
      541 tggatcgcaa cctcaacgtg atgatgttct ccgataacgt cacgctggaa gatgaaatcc
      601 aacttaaaac ccgcgcgcgg gaaaaaggct tgctggtgat ggggccagac tgcggtacgt
      661 cgatgattgc tgccacgccg ctggcttttg ctaatgtgat gccggaaggc aacattggcg
      721 tcataggcgc ttccggtacc gggattcagg agctgtgctc acagattgcg ctggcagggg
      781 agggaattac tcacgcgatt ggtcttggcg ggcgcgatct cagccgtgaa gtgggcggca
      841 tcagtgcgct aacggcgctg gaaatgctca gtgcagacga gaaaagcgaa gtgctggcat
      901 ttgtttcaaa accacctgcc gaaactgtgc gtctgaaaat tgttaatgcc atgaaagcaa
      961 ctggcaaacc gacggtggcg ctgtttttag gttatacccc ggcggtggcc cgcgacgaga
     1021 atgtctggtt tgcctcctcg ctggatgagg ccgctcgtct ggcttgtctg ctttcacgcg
     1081 tcactgcgcg acgtaacgcg atagcgcctg tcagcagcgg atttatttgc ggtttgtata
     1141 ccggcggtac gctggctgct gaagcggcgg gattacttgc cggacacctt ggcgtggaag
     1201 ccgacgatac ccatcaacat ggcatgatgc tggacgccga cggacatcag atcctcgacc
     1261 tgggcgatga tttttacacc gtcgggcgtc cccatccgat gatcgacccg accttacgca
     1321 acctgttaat tgccgatctc ggcgctaaac cgcaagtgcg agtgttgctg cttgatgtcg
     1381 tgattggctt cggcgcgacc gccgatcctg ccgcctcgct ggtgagcgcc tggcaaaaag
     1441 cctgtgccgc gcgttcagac aatcaaccac tgtatgccat tgccacggtg acaggcactg
     1501 aacgtgaccc gcaatgccgc tcgcagcaaa tcgccacgct ggaagatgcg gggatcgcgg
     1561 tagtgagttc gctaccggaa gccaccttgc tggctgcggc gttaattcat ccgctctcgc
     1621 ctgccacaca gcaacacaca ccgtcattac tggaaaacgt cgccgtgatt aacatcggat
     1681 tacgcagctt tgcgctggag ctacaaagcg ccagcaaacc ggttgtgcat taccaatggt
     1741 cgccagtcgc cggcggcaat aaaaaactgg ctcgtttatt agaacgtttg caataagggg
     1801 ttcccatgtt tacatcagtg gcgcaagcca atgctgcggt tatcgaacaa attcgtcgtg
     1861 ctcgtccaca ctggctggat gtgcaaccgg cttcttcact tatcagcgaa ctaaacaagg
     1921 gcaaaacact gcttcacgcc gggccgccaa tgcgctggca ggagatgacc ggacccatga
     1981 aaggggcgtg cgtgggcgca tgtctgttcg aaggctgggc gaaagatgaa gcgcaggcgc
     2041 tggcaatact ggagcagggg gaagtgaact tcattccttg tcaccatgtg aatgccgtcg
     2101 ggccaatggg cggcattact tctgccagta tgccgatgct ggtggtcgag aacgtgaccg
     2161 acggcaaccg ggcgtactgc aacctcaacg aaggtatcgg caaagtgatg cgttttggcg
     2221 cttacggcga agatgtcctg actcgccatc gctggatgcg cgatgtgtta atgccagtat
     2281 taagcgcggc gctggggcgc atggagcgcg gtatcgatct cacggcaatg atggcgcagg
     2341 gcattaccat gggcgatgag ttccatcaac gcaatattgc ttcctcagca ctgttaatgc
     2401 gtacgctggc cccacaaatt gcttgcctcg atcatgataa acagcacatt gcggaagtga
     2461 tggattttct cagcgtgacc gatcagttct tcctcaacct cgcgatggcc tactgcaagg
     2521 cggcgatgga tgcaggcgcg atgatccgcg caggcagcat cgttacggct atgacccgca
     2581 acggcaatat gttcgggatt cgggtaagcg ggctgggtga acgctggttt actgcgcctg
     2641 taaacactcc gcaaggtctg tttttcaccg gcttctcgca ggagcaggcg aacccggata
     2701 tgggcgatag cgcgattacc gaaacctttg gtatcggagg tgcggcaatg atcgcagcgc
     2761 ctggcgtaac gcgctttgtc ggtgcgggtg gcatggaagc ggccagagcg gtatctgaag
     2821 agatggcgga aatttacctt gaacgcaata tgcagttgca gatccccggc tgggatttcc
     2881 agggggcgtg cctggggctg gacattcgtc gcgtggtaga aaccggcatt acgccactca
     2941 tcaataccgg tatcgcccat aaagaggcgg ggatcgggca gattggcgca ggcaccgtgc
     3001 gggcaccgct ggcgtgcttt gaacaggcgc tggaagcact ggctgaaagc atggggattg
     3061 gttgaggaac gcacaatgac gatcatccat cctctgcttg ccagccgtag cgcaccgaat
     3121 tatcgccagt cctggcggtt agcgggagtg tggcggcggg cgattaacct gatgacggaa
     3181 agcggcgaac tgttaacgct gcatcgtcag gggagtggtt tcggccccgg cggatgggtg
     3241 cttcgccgcg cgcaattcga tgcgttatgc ggtggattat gcggcaatga acgaccacag
     3301 gttgtggcac aaggtattcg tctcgggcgt ttcacggtta aacagccaca gcgttattgt
     3361 ttgctacgta ttacgccgcc tgcgcatcct caaccgcttg ccgttgcatg gatgcaacgc
     3421 gcggaggaaa ccgggctttt cgggccactg gcattggcgg caagcgatcc gctgcctgct
     3481 gaattacgcc agtttcgtca ctgttttcag gccgcgctca atggcgttaa gaccgactgg
     3541 cggcactggc tgggtaaagg ccccggatta acgccaagtc atgatgacac gctgagcgga
     3601 atgctgctgg cggcctggta ttatggcgct ttagatgcgc gctccggtcg cccgtttttt
     3661 gcctgttccg acaatcttca actcgttacc actgcggtga gcgtcagtta tttacgttat
     3721 gccgcgcaag gatatttcgc ctcgccactc ctgcactttg ttcatgcgct gagttgcccg
     3781 aaacgtaccg cagcggcgat cgattcgctg ctggcgctgg ggcatacgtc aggggcagat
     3841 acgctgctgg ggttctggct tggccaacaa ttattacaag ggaaaccatg aaaacactgg
     3901 ttgtggctct tgggggcaac gccttactcc agcgcggtga ggcgctgacg gcaaaaaatc
     3961 aatatcgcaa tattgccagt gctgtacccg cgctggcgcg cctggcccgt tcttatcggc
     4021 tggcaattgt tcacggcaac ggaccgcagg tggggctgct ggcgttacag aatctggcgt
     4081 ggaaagaggt agaaccgtat ccgctggatg tactggtggc ggaaagccag gggatgattg
     4141 gctatatgct ggcgcagagc ttgagcgcac agccgcagat gccgcctgtc acgacggtgc
     4201 tgacgcgcat tgaggtttcg cctgatgatc cggcgttttt acagccagag aagtttatcg
     4261 gtccggttta tcagccagaa gaacaagagg cactggaagc ggcttacggc tggcagatga
     4321 aacgtgatgg taaatattta cgtcgggtgg tggcgtctcc gcaaccgcgt aaaattctcg
     4381 acagcgaagc catcgagttg ttgctcaaag aggggcatgt ggtgatttgc agtggcggcg
     4441 gtggtgtgcc tgtgacggaa gacggggcag ggagtgaagc ggtgattgat aaagatctcg
     4501 ccgctgcgtt gctcgccgag cagattaatg ccgatggact ggtgatcctc accgatgctg
     4561 atgcggtata tgaaaactgg ggaacgccgc agcaacgtgc cattcgccat gccacaccgg
     4621 atgagttagc gccatttgcc aaagccgatg gttcgatggg gccgaaggta acggcggtga
     4681 gtggctatgt cagaagccgt ggtaaacccg cgtggattgg ggcgttatcg cggattgaag
     4741 agacgctggc gggcgaagcg gggacttgta tttcgctgta gtgatgggca ccaggcaatt
     4801 ggcacatct
//
LOCUS       DACADF010000237         4808 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.239, whole genome shotgun sequence.
ACCESSION   DACADF010000237 DACADF010000000
VERSION     DACADF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.239"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            104..571
                     /locus_tag="ISB30_004595"
     CDS             104..571
                     /locus_tag="ISB30_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1992150.1"
                     /translation="MLKTNSLRESMLHGCRWCQANPEKFTIFVESGNIETTGEAPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRNSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            564..1199
                     /locus_tag="ISB30_004596"
     CDS             564..1199
                     /locus_tag="ISB30_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HAP1992151.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTEWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            1196..1777
                     /locus_tag="ISB30_004597"
     CDS             1196..1777
                     /locus_tag="ISB30_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HAP1992152.1"
                     /translation="MSAELMRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNT
                     TRAGAFNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDASPAPGKSLKEIVVSAPDG
                     AVFRYDADAGALSASGMKTATLQASVSVTLDTPVVECTNLLRTATLDVTKEGKMSGNI
                     THSGGNFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            1774..2124
                     /locus_tag="ISB30_004598"
     CDS             1774..2124
                     /locus_tag="ISB30_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP1992153.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILITPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            2128..3024
                     /locus_tag="ISB30_004599"
     CDS             2128..3024
                     /locus_tag="ISB30_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HAP1992154.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHGSGTNLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PACVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSASIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            3017..3547
                     /locus_tag="ISB30_004600"
     CDS             3017..3547
                     /locus_tag="ISB30_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HAP1992155.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGARITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIQGDIFA
                     AAATYDGEVITIYPDD"
     gene            3550..>4808
                     /locus_tag="ISB30_004601"
     CDS             3550..>4808
                     /locus_tag="ISB30_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1992156.1"
                     /translation="MSTTTRKFKTVITDTGAKKLAQAAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVVLTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNSPALTGTPTTPTARQGTNNTQIASTAFVMAAI
                     AALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQPKDATLTALAGLTTAAGKF
                     PYFTGNDVASLATLTKVGRDILAKSTVA"
BASE COUNT         1178 a         1334 c         1383 g          913 t
ORIGIN      
        1 taactgatat cactaaaaat gccgcgtacg gcgtcgatat cggcagcctg gtgctggaaa
       61 tcaataatcc cgcagcataa ggaagagcag gagagcaaaa cagatgctta agacaaacag
      121 tctgcgagag tccatgcttc atgggtgtcg gtggtgccag gctaatcccg agaaattcac
      181 cattttcgtg gagagcggca acattgaaac gaccggagaa gcgccctcgt ttgtttaccg
      241 ctatcagatg gtgatgtttg tcatggatta cgccggagag ctggacgacc tcacgctgcc
      301 gctgctggcg tggttatccg aaaatcagcc acagttgttg ctcaatccgg agcgtaatca
      361 ggacatcaaa ttttctgccg ttatcaatga cgatgacagc gccgatctcc tgtttacgct
      421 ccccctgcgg gaacgcgttc gcatcacgcg caacagtcag ggcacaccgc aggcagaaca
      481 cctgccggag ccaaaacccc gtctgccttc ttccgaaggc gactggtcgc atgtattcca
      541 ggatgtgacg tggggtgaaa gcgatggata aggcattcac ccgcgtggat gaaacctttg
      601 aggctatccg cgacagcctg aatcagcagg ccatcaataa catcgccaga aagctggcac
      661 aggatttacg tcgcgcccag caggcacgta tccggtcaca aaaagcgccg gacgggaccg
      721 aatggacacc acgcagacgc cgcgtaaccc ggatacagga gcgcattcgc tttatctgga
      781 ataacgaagc acgcacgctg aaaaactggc atcacgacac agggaaatac gggcgaacca
      841 ttaccgggtg ggatgaggat aaaaacaata tccgcacgtt ttaccgggat gacatcgacc
      901 gttttctgga aatacgcacc cggcgcatca accaggacag cacaaagcgc gtccccatgt
      961 tcgtaaaact gcgcaccgcc cgctacctga aagcccgtgc agatgcctcc ggtgtgacgg
     1021 tgggttacag cggcgtggcc gcacgtattg cacgcgttca tcagttcggt gagcgcgatc
     1081 aggttgcgcc gggcattttc accgattacc cggtacgtga gctgttgggt atcagccagg
     1141 cagatgagcg cctgatttat aacacggtgc tgggccggat tgcggaggct gtacggtgag
     1201 cgcagaactc atgcgactgc tgagcaacat catccgcacc gggatcatct ctgaagttga
     1261 tgagaagtcc tggcgcgtgc gcgttcgcag cggcgaactg gaaacaggct ggttgcgctg
     1321 gaacaccacg cgcgcgggag ccttcaatgt gtggctgccg ccatcaccag gcgaacaggt
     1381 ggtaattgcc tgcattggcg gcaacccgga aaccgccatg ataattggca gcctgtggag
     1441 tgatgccagt ccggcccccg gcaaaagcct gaaagaaatc gtggtcagcg cgccggatgg
     1501 cgcggtgttc cgctacgacg cagacgctgg cgcactgagc gccagcggca tgaaaacagc
     1561 caccctgcag gcatccgtca gcgtgacact ggatacgccc gtcgtggaat gcacaaacct
     1621 tctgagaacg gcgacgcttg acgtcacaaa agagggaaag atgagcggca atatcacgca
     1681 cagcggcggc aatttcacct caaacggcat taccgtgcat acgcataagc acggtggcgt
     1741 taaaggtggc agcgattcga caggaggccc gcagtgacaa cccgctacac aggaatgaac
     1801 ccggacggga cgggaaacct gaacgatatg gagcacctga aacagtcagt cagggacatc
     1861 ctgatcaccc cgctggcaag ccgggttatg cgacgggaat atggcagcct tgtgcctgat
     1921 ttgattgacg aacccatgaa taacaccacg cgtctgcaat gcatgagtgc tgccgtgatt
     1981 gcgctgacac gatgggaacc ccgcattgcc ctggacgcca tcgacgttgt ctggaaggca
     2041 ggaggccgcg ccggggtgac gctgtcgggc actgtcatgc agaccatgca gaatgttgaa
     2101 ttaaccatca cgctgagaga gtaaatcatg cctgctgttg atctttccca gttaccggaa
     2161 cccgccatca tcgcggagcc tgattttgag gcaattctgg ctgacacaaa ggccatgatg
     2221 attgcgtcct atcctgccga acagcgtgaa gccgtctccg ccgcgctgga gctggaatca
     2281 gaacccctga acgttatcgc tcaaaccatg tcgtttcgtg aaatgctgtt acgccagcgg
     2341 gttaacgagg gcgcacgcgc ctgcatgtta agccacggtt cagggacaaa cctggacaac
     2401 ctcgcgggca atatgaacac aaagcgcctg gttatcactc cggcaacgga taccaccgac
     2461 gcggtgatgg agagcgacac ctcgctgaga ctgcgggcgc agcgggcgta tgacggcctg
     2521 agtgttgctg gcccgtcagg tgcatacgag tattttgcac gcagcgccag cggtctggtg
     2581 cgtgatgcgc gggccatcag cccgtctccg gcctgtgtga cggtttccat tctgtccact
     2641 gaaggcgacg gcacagcaac ggaggcgttg cttaataccg ttcgcgccgt tctgaatgca
     2701 gaggataccc gcccggtggc cgaccgactg accgtacaga gcgccagcat cgtgacatgg
     2761 cggctgaatg caaaactgta cttttacccc ggcccggaat ccgaacctat tctggctgcg
     2821 gctgaatcgt cgttcaggaa gtggctggct gagcaggggc ttattggtca ggacgtggcg
     2881 ttgtcagcca ttgctgccgc acttcatgtg cacggcgtgc aacgcgtgga gataatcgaa
     2941 cccacacaga atatggccat cagcgacata caggcggcgc gctgtgagtc attcaccatc
     3001 agcgaaggtg ggcgtaatga gtaattcact gttaccgcca tcagccagca atttcatgcg
     3061 ttgcgccgaa gctgtcggag cgcgcattac agacatcccg gtagacctca acacgctgtg
     3121 gtcaccggac acctgcccgg tgcatctgct gccttatctc gcctgggcat tttccgttga
     3181 ccgctgggat cgcaactggc cggaagagac aaagcgacag gtgattcgtg atgcatggct
     3241 gatacaccga cacaaaggga ccatcagcgc actgcgccgg gccattgagc cgctgggata
     3301 cctcattcgc gtgtctgagt ggtgggagtt cggcggagaa ccgggaacat ttaccgttga
     3361 agtcggcacg ctggacagtg gcgtgacgga ggaaatgtat ctggaaatgg agcggttgat
     3421 tgctgatgcc cgcccggtca gccgccacat gacagggctg aatatcattc aggagattca
     3481 gggagatatt ttcgcggcgg cagcaactta cgacggcgaa gtcattacca tttatccgga
     3541 cgattaagca tgagtaccac aacacgtaaa tttaaaaccg ttatcaccga tacgggtgcc
     3601 aaaaaattag ctcaggcagc cgcgccagat ggtaagcctg tccgcctgac tcatatggcc
     3661 gtgggcgacg gtggcggcac gttgcccaca ccagacagta agcagacccg tctggtgcat
     3721 gaggtgtggc gacatactgt taatcgcgtc atcctggacg caacacatca gaaccgcatt
     3781 attgcggagc tggttattcc tcctgaaacg ggcggattct ggatccggga aattggtgta
     3841 tttgatgagc acggcgattt aatcgcggtg ggcaatactg ccgaaagtta caagccagcc
     3901 gttgccgaag ggtccggtcg tgcacaaaca tttcgcacca ttctgaccgt atccagcact
     3961 gccaccgtgg tgcttaccgt ggataacacc atggtgatgg ccacagtgga ttacgtggat
     4021 gacaaactga aagagcatga acagtcacga cgtcacccgg acgcctcgct gaccgcaaaa
     4081 ggctttgttc aactcagtag cgccactaac agcgtgtctg aaacgcaggc tgcaacgccg
     4141 aaagcagtaa aggccgcgta tgaccttgct aacggtaaat atactgcgca ggatgccagc
     4201 acgacgcgaa aaggccttgt ccagctcagt agcgccacca acagcacgtc tgaaacgcag
     4261 gctgcaacgc cgaaagcagt aaaggccgcg tatgaccttg ctaacgcaaa atataccgct
     4321 caggacgcca cgacggcaca aaaagggata gtccagctca gtagtgccac caacagcacg
     4381 tctgaaacac tggccgcgac atcgaaagct gttaaggcgg taatggatga aacgaacaag
     4441 aaagcgccct taaatagtcc tgcactgacc ggaacgccaa caacaccaac tgcgcgacag
     4501 ggaacgaata atacccaaat cgcaagcacg gctttcgtta tggctgcgat tgccgccctt
     4561 gtagattcgt cacctgatgc actgaacacg ctgaacgagc tggctgcggc gctgggcaac
     4621 gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc gaaagatgcc
     4681 accctgacgg cgctggccgg acttactact gcggcaggca agtttccgta ttttacgggg
     4741 aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct tgcgaaatcg
     4801 accgttgc
//
LOCUS       DACADF010000238         4594 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.240, whole genome shotgun sequence.
ACCESSION   DACADF010000238 DACADF010000000
VERSION     DACADF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.240"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..250
                     /locus_tag="ISB30_004602"
     CDS             <1..250
                     /locus_tag="ISB30_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP1992157.1"
                     /translation="TNDNVPGLLSLITAHLKDLPDDGRNEDVFKMLRSSAAILHGINN
                     LRNNYSMAHPTETLLNEADARFAINLVRSIMTYVDELL"
     gene            complement(539..1350)
                     /locus_tag="ISB30_004603"
                     /pseudo
     CDS             complement(539..1350)
                     /locus_tag="ISB30_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
     gene            2136..3113
                     /locus_tag="ISB30_004604"
     CDS             2136..3113
                     /locus_tag="ISB30_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP1992158.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3398..4138)
                     /locus_tag="ISB30_004605"
     CDS             complement(3398..4138)
                     /locus_tag="ISB30_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP1992159.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(4259..4447)
                     /locus_tag="ISB30_004606"
     CDS             complement(4259..4447)
                     /locus_tag="ISB30_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HAP1992160.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPALSPRHDWL
                     NTGSLLQNIPLRIWFYPQ"
BASE COUNT         1134 a         1175 c         1131 g         1154 t
ORIGIN      
        1 tacaaatgat aatgttccag gcttgctttc cctgataact gcacatctca aagacctgcc
       61 tgatgatgga agaaatgaag atgtgtttaa aatgttgaga tcatctgccg caatacttca
      121 tgggatcaat aacctgcgta acaattacag catggcccat cctactgaaa ctttgctgaa
      181 tgaagccgat gcccgttttg ccataaatct ggttcgttcc atcatgactt atgttgatga
      241 gttgctttag ttttaataaa aatatgagac tcacagcgaa cagatagaac ttcaacgaag
      301 aagtgaagta cggccagaat ttcgagttct ggccctccgg aagatgccgg acagacgcat
      361 catcccgtcg tggccgcttc catttttgtg tgtactcaac ctgccggatc aggatggcgg
      421 caggatttgc acccgatgtt ccagccgcct tgcctcttcc tgatcccggt attccctcgc
      481 ctttttcatc atttctgccg ttggctcctg cagtttgtcg agaaactcct tagatcgttt
      541 agccaccgac tgaagggaga cactgtccgt cctgccggta ttgcgattgg tgtaaaccag
      601 gcgggtaagg gggtctggtt aactccttcc cttccataaa gcgtttgaag atccagagat
      661 atatatcaac cagctccagc cctgcacttt ttgtacccga ctggaaaacc agcgttcagc
      721 cggcatgttc gtcacatcca tgaccggtaa accgggaccg tgtacccacg gcatctcacg
      781 tatctggtaa tagaactccc tcagctcccg ctgggtggtg ttgaactgtg actgctggtc
      841 cacaacgatg tcggctttgc gatttggtgc tcccaggcgg gaacagatac cgcgcaatac
      901 gaactgaaat ccaaccatat tgggcatgat ctgcagcttt tgtttaccgg ttttgcagtt
      961 atatcccagt tcgtcaaagt gggtcatcgc ccaggacaac gcatcggtaa tcaattcaat
     1021 gtaacggcgg tcccccaggg tatgaacgcg cgcaagcagc tcactgcaga cctcactgaa
     1081 cagagatgag gaccttccat catgggcttc gaggcgggca caccacgctt tttcagccag
     1141 ttcattatcg aacaggcttg ccagattcag tagcagcggg taacgaaagg gggtccagta
     1201 ggcaaccctg ggcactgcag gattcatccc ctgatcgaag aacgaaataa tggcgtgatc
     1261 ccgtttaacg acctgccaga catcaaaccg tatccggtgc ttcttctgca ggacaagcag
     1321 cgtatcaacc acctcatcaa ggcggtgcat gccaagctct gcggcgtgca gacgctgtac
     1381 ttgtcaacgc cacgatcttt gaccgtctta tatcgacaga gttatccggc atggtgtcaa
     1441 tccgttcaca atttttccat cggagcagcc taccaccggt gataaccaga ctttgcctgt
     1501 agtttgtata cacaatccct ggcattgaaa cacacatatg aagtgtactt cggatatgac
     1561 cgtacgctca ggaagtacgg cagattccgt caaaaattac ctgtaaagac agacatcaga
     1621 aaaacagtgc tcgccgcaga ccggtgaccg ggcgaagccc gcgaaccggc gaggaagcgc
     1681 aatggatctg tagttacagt gagcaggtac gccctgaagc cttcataatc ggtagtgtat
     1741 gctgtttttc tggaatgatt tatccacata tccggaggcc cgatccagta aacagatcca
     1801 tgaatgatca acaaaggatc cattaaagat ccccatatct ctgcaaacct tgtcactcat
     1861 gggccgggac tacgatcaca taagctgtgg catgttactg ataaactgta acatgctaat
     1921 gataagctgt attcagtaat ccatatactg aagtaagtta atgacataaa ctatggtcag
     1981 tatgccagac tcagttgtta aatacaggct gcaggttttt cttcagtcag ttagcggagt
     2041 tctgacacac gattttctgt ttattctttt actgtccaca ggctggaggc tttctggaaa
     2101 acgaaaattc agacatcaaa aaactgttcg gcgaggtgga taagtcgtcc ggtgagctgg
     2161 tgacactgac accaaacaat aacaacaccg tacaacctgt ggcgctgatg cgtctgggcg
     2221 tctttgtacc gacccttaag tcactgaaga acagtaaaaa aaatacactg tcacgcaccg
     2281 atgccacgga agaactgacg cgtctttctc tggcccgtgc agaaggattc gataaggttg
     2341 agatcaccgg cccccggctg gatatggata acgatttcaa gacctgggtg gggatcattc
     2401 attcctttgc ccgccataac gtgattggtg acaaagttga actgcctttt gttgagtttg
     2461 caaaactgtg tggtatacct tcaagccagt catcccgcag gctgcgtgag cgcatcagcc
     2521 cttccctgaa gcgcattgcc ggtaccgtga tctcgttttc ccgtaccgat gagaagcaca
     2581 cccgggaata catcacccat ctggtacagt cagcctacta cgatactgaa cgggatattg
     2641 ttcagttaca ggctgatccc cgcctgtttg aactgtacca gtttgacaga aaagtccttc
     2701 tccagcttaa ggcgattaat gccctgaagc gacgggagtc cgcccaggca ctctacacct
     2761 ttatagagag cctgccccgg gatccggcac cgatatcgct ggcgcggctg cgtgcacgcc
     2821 tcaatctgaa gtctcctgta ttttcccaga accagacggt cagacgggca atggagcagt
     2881 tgcgcgagat tggatatctt gattacacgg agatccagcg ggggcggaca aaattcttct
     2941 gtattcacta ccggcgtccc cggttaaaag cgccgaatga tgagagtaag gaaaatccgt
     3001 tgccaccttc acctgcggaa aaagtcagtc cggagatggc ggagaaactt gccctgcttg
     3061 aaaaactggg catcacactg gatgacctgg aaaaactctt caaatcccgc tgaacataaa
     3121 ctgtagtcag tgaagagtgc tcctttactg actacagctt atattatcag gtgcagtgag
     3181 tggtctgctc actgctgttt atattcagtt tcctgcagtg ctgcctgtgg ctgagctgcc
     3241 atctgcctgt cccttacgtg agtcaccccg taacctgatg ctgaggcatt gctcccttca
     3301 taaaacatgg tttactcact acagcttatc tacctgctcc agcttatgtt atgtctgttc
     3361 tgctgaccac agcttatata aggaagcgcg tatgccgtca cttcagggga ggcagtgtac
     3421 gcaggatctc cgcagcatcc cgaccgtcac ctgtgaaagg cactgccagc gtggcagcca
     3481 tatccagtgc aaacactctg gtataaacct ccatcgaacg tggatccctg tgaccagcca
     3541 gtgcctggat gactttccgg ggctggcggt gatagagcat gtgcatgata tagctgtgcc
     3601 ggaaggtgtg tggtgtgacc ggaatcgaaa agtgtacccc gtcagcttcg gcccgtctga
     3661 cagcctgctt cagccagttg cgcatggttt cgtcggtcac ggcccataat ggttcacgac
     3721 gacggggccg ggtggtgatc atccagcttt ccatctgcct gacatagctt atatctgtca
     3781 gcggaaccag gcgcacttca tcttttggcg ggcgtccgcg tcgcgcacgc actttttcgg
     3841 acaggatccg cacaaacggt cttactccat ccaggtcaaa tgattccggc gtcagcatcc
     3901 gggcttcgcc aatacgcatt ccggtattcc agagggtggc gaacagcata tggtgacgct
     3961 gatccggcat atagaagaga agggcactca cttccggggc cagcaggtag gccggtgtgg
     4021 cccccgtgga agtggccatt cttctcaggg agagcgctgt tgcaaaatcc acccccggcg
     4081 caatgggtaa cagggagacg cgttctggtg aattctgcag gggaatgaca ttgttcatga
     4141 gttatttttc acgtagcaca cgcactgttt atggatacag gtattctgac ctgaacgccc
     4201 ggatccttcc acgacaataa atcgcagcgc gcggatcctt ttgtttgctt atcacgcatt
     4261 attgcgggta aaaccaaata cgtaaaggta tgttttgcag cagggatccc gtattaagcc
     4321 agtcgtggcg cgggctgagg gctggtttgc cgtttttggg agaattgggg taatgccagg
     4381 aacaggcaga tacggagaaa aatcctgcag ataaccttct acgacaactg aaggtgtctg
     4441 acgccatatt cttagctact tctcagtttt aaggcggctt agtcacaaca taaggttatg
     4501 cataataagc atattttatt atgttatgtt atggtcttgt tcatgcatga actgttatct
     4561 attcagtcac ttttaacgtt ttatatcata ctgc
//
LOCUS       DACADF010000239         4517 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.241, whole genome shotgun sequence.
ACCESSION   DACADF010000239 DACADF010000000
VERSION     DACADF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.241"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            242..1369
                     /locus_tag="ISB30_004607"
     CDS             242..1369
                     /locus_tag="ISB30_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992161.1"
                     /translation="MVEKMKQNSSSTLGALSAPTTWPGANARLLGLGVGLPIAPLDRL
                     AQFSAAEFERFTLEWASEYLAGQQYVNEVQQRGGAGDKGRDIVVWLDPSDAVPRRWHL
                     YQCKHYDANLGLPKAGIEVAKVIYYTFIKDYTVPTQYHFVTHKGVTSPFQDLLDDPTK
                     LKEKMLSEWATFSKQITSKHSVDLTPELEKYIKEFDFSIFHAKQPIEILAEHSKTSFH
                     LMVFGAPLIERDPPTRPPSSVAPIETVYIEQLFSVISADIKTNVRDLVDFQSSISHVK
                     LFERSRITFYCSEGLKELARDQMANQEFFNSLLVEFDDGLYHYTSDLTGTPLLRLKNT
                     VKAAQTLQLGAHPLAIHVTNKDREGICHQLANTNLINWCNP"
     gene            1366..1881
                     /locus_tag="ISB30_004608"
     CDS             1366..1881
                     /locus_tag="ISB30_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992162.1"
                     /translation="MSEMQVFEGNFEQRPFNTPLEYGFRALFILDAADNVAMDLQRIV
                     SYDYLLVHTSDVPRGPKSLHPAVPHRGAELLVKRSAIQAGLHLMLSRELIKVVFAAEG
                     ILYQATNLTGRFVRLLMSQYSKELAERASWVTKQFYEYTDEELASYISQNVGQWGSEF
                     DRLTAIDLLDL"
     gene            1883..3769
                     /locus_tag="ISB30_004609"
     CDS             1883..3769
                     /locus_tag="ISB30_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992163.1"
                     /translation="MLRIRKILLRGNSVQDAYVDFYKGANILAGESDTGKSYLVSCLD
                     YILGAEKLKKKLKEATDYTHLYVEFENDEGGVLTLKRGLEGGKLEAHDVAIQDIHGEG
                     KIIAPVRKGTSKGPDVTSILFPFAGIKEAKLRKNARGETQRFSIRTLAPIFLVDEVSI
                     IDEYSPVTGRSGYDDTARKRMFSYILTGHDDGGVTVEEKPEIVKARLMAKLEFIQDLI
                     RPLDERFSICSPKFPLTSSADDLSDQLIAQAIDEVERAAAAISDLLEGIKMETALTLK
                     IESQLMGVSEIQSRYSLLEERYHSDLKRLDFISEGSHYFTSLQEVPCSLCGQNLLHPH
                     SENAKKLMNSNEVRRSSLAEAAKIHGYLAGLQKAMSDLDRRKEALNIDRYKSKESLDG
                     MKNQIKYTFEPLLTLSKESLDLAIEQRAERDSELNDRQRWFELLKHQKDIEILLEKSS
                     QPRLEWEGLSTRTLAELCREIETVLKDWKWGAEPDVSFNEKEYDIIVDGQPRQSHGKG
                     VRAILYSAFIIGLLKYCIKKGYPHPGLVVIDSPLTSYKKKAAATVSGFDEPISAGIEE
                     GFWHSLTQLPDTLQIIVIENKEPPVSIMNKVHYEWFAGEHAMDGERRALIPLPSKNSA
                     ILMK"
BASE COUNT         1320 a          897 c         1068 g         1232 t
ORIGIN      
        1 atgaaaagag ataaaaatgc tgatgtcaaa agaggcttat gctcggcaca gtggagtgag
       61 ctgccaaact gtcgatgact gggtagccgg tggggcggaa gtagttatgt cccgtagcaa
      121 ggttaagatt tgctcttgtg tgtggggaac cttagtcaat tactttcctg gcgcactgtg
      181 ttagattttg taaaatttta aaagactaaa gatttaatat cacttctcca tggaggttgt
      241 ggtggtagag aagatgaaac aaaattcaag ttcgacgctt ggcgcgcttt ctgctcctac
      301 aacgtggccg ggtgcgaacg ctcggctgct tgggttaggt gtagggcttc caatagcccc
      361 cttggatcgt ctggcacagt ttagcgccgc cgaatttgaa aggtttacac tcgaatgggc
      421 ctctgaatac ttagctggtc agcaatatgt caacgaagta caacaacgtg gcggggcagg
      481 agataaaggc agagatattg ttgtatggct tgatccatct gatgctgttc ctcgaagatg
      541 gcacttatat caatgcaaac attatgatgc gaatttaggc cttcctaagg caggcatcga
      601 agttgcaaaa gttatctatt atactttcat caaggactac acagtaccta cacaatacca
      661 ttttgttaca cataaagggg taacatcgcc attccaagac ctcctagatg atccaactaa
      721 actcaaagag aaaatgctat ccgagtgggc aactttttca aagcaaatta catcgaaaca
      781 tagtgttgac ctgacgcctg aacttgaaaa atatattaaa gagtttgatt ttagtatttt
      841 tcatgcaaaa cagcctattg agatacttgc cgaacacagt aaaacctctt ttcatcttat
      901 ggtctttggc gcaccattaa ttgagcggga tccaccaact aggcccccat catctgtggc
      961 tcccattgaa actgtatata ttgaacaact cttttcagtt atcagcgctg atatcaaaac
     1021 caacgtgcga gatttagttg attttcaatc aagtatcagc catgtaaaac tctttgaacg
     1081 gtcccgaata actttttatt gctcggaagg gctaaaggaa ctcgctaggg accaaatggc
     1141 gaatcaagaa ttctttaatt ctttacttgt agagtttgat gatggtctgt atcactacac
     1201 ctctgacctg acagggactc cactccttcg gttaaaaaat accgtcaagg ccgctcagac
     1261 cctgcaactt ggtgcgcacc cacttgccat tcacgtaacg aataaggatc gtgaaggtat
     1321 atgccaccaa ctagccaata caaacttgat taattggtgc aatccatgag cgaaatgcaa
     1381 gtatttgaag gaaactttga gcagagacct tttaacactc cgctggagta tggatttcgt
     1441 gctctgttca tattggatgc agcggacaat gtagcaatgg acctacaaag gatagtttcg
     1501 tatgactatc ttcttgtgca taccagtgac gtacctaggg ggcctaaaag ccttcatcca
     1561 gcagttcctc accggggagc agagcttctt gttaagcgaa gcgcaattca ggcagggctg
     1621 catttaatgc tatcaagaga attgattaag gttgtctttg ctgccgaagg tattttgtat
     1681 caggctacca atttaacagg aaggtttgtg cggcttttga tgtcacagta ttcaaaagag
     1741 ctcgcagaac gtgcctcttg ggtcacaaag caattctatg aatatacgga tgaagaactt
     1801 gcttcatata tatctcagaa tgtggggcaa tggggttctg aattcgaccg attgaccgca
     1861 attgatctac tggatttata acatgcttcg tataagaaaa atactattgc gcggaaacag
     1921 cgttcaagac gcttatgttg atttctataa gggtgcgaat attctagcag gggaatctga
     1981 tactgggaaa agttaccttg tgagttgtct tgactacatt cttggtgccg aaaaactcaa
     2041 aaaaaagctt aaagaggcca ccgattacac ccatctttat gttgaattcg aaaatgatga
     2101 agggggggtg ttaacgctca agcgcggtct tgaggggggg aaattagagg cacatgatgt
     2161 cgctattcaa gacatccatg gagaaggtaa aataattgct cctgtgcgta aaggaacgag
     2221 taaggggcca gatgtgacgt ccatactttt cccttttgcg gggatcaaag aggcgaagct
     2281 tcgcaagaat gctcgtggcg aaacgcaacg tttctcgatt cgaacgctcg ctcctatatt
     2341 tttggtagat gaagtatcca ttatcgacga atactcgcca gtaacgggac gttccggtta
     2401 tgacgacact gctcgaaaac gcatgttttc atacatcctt accgggcacg atgatggtgg
     2461 tgttaccgta gaggaaaagc ctgaaattgt aaaagcgagg ttgatggcga agcttgaatt
     2521 catccaggat ttgattcgac cacttgatga acgtttctca atttgttcgc ccaagtttcc
     2581 attaacgagc tcagcagatg atttatcaga tcaattgatt gcacaagcaa ttgatgaagt
     2641 tgagagggct gcagccgcta tttctgactt gctcgaggga ataaaaatgg agacagcgtt
     2701 aacgttaaaa atagaatctc agttaatggg agtatctgaa attcaatcga gatatagcct
     2761 attagaagaa cgttaccatt ccgacctgaa gcggttggat tttatttccg aaggttctca
     2821 ctatttcact tcacttcagg aagttccttg ttccctttgt ggacaaaatt tactacatcc
     2881 acacagtgag aacgctaaaa aattaatgaa cagtaatgaa gtccgacgtt cttctttagc
     2941 tgaagctgca aaaattcatg gataccttgc aggcctgcaa aaagcgatga gtgatcttga
     3001 ccgaagaaaa gaagctctga atatagacag atataagtca aaagaaagct tggatggaat
     3061 gaagaaccag atcaaatata cttttgagcc tcttcttaca ctttccaaag aaagcctaga
     3121 tttagctatt gagcagcgag ctgaaagaga ctctgagttg aatgatcgac agagatggtt
     3181 tgagttacta aaacatcaaa aggatataga aatactgctg gaaaagagca gtcaaccacg
     3241 gctcgaatgg gagggattat ctacccgtac gctggccgaa ctttgtagag aaattgaaac
     3301 tgttttaaaa gactggaaat ggggtgccga gcctgacgtt tcatttaatg aaaaagagta
     3361 tgacatcatt gttgatggac aacctcggca gtctcacggt aaaggtgttc gtgccattct
     3421 ttattcagct ttcatcattg gattactaaa atattgtatt aaaaaaggat acccgcatcc
     3481 tgggctagtc gtgattgatt caccgctgac aagttataag aagaaagccg ctgctacggt
     3541 atcgggcttc gatgaaccca tatcggctgg tattgaggaa ggcttctggc attcactgac
     3601 ccaattgcct gataccttac aaatcatagt tatagagaat aaagaaccgc cggtatcaat
     3661 catgaataaa gttcattacg aatggtttgc gggtgaacat gcaatggatg gagagcgcag
     3721 agcccttatc ccgctgccct caaaaaatag tgctatttta atgaagtaac ttaggatggg
     3781 tagccatcaa atcgcagtta tacgattggt ggggtttgta aaagcggtag tcttgattac
     3841 cactgcattc attctacaag cacatacttc cctaggttgc tgtagttatc cgattacacg
     3901 ccttttctga aagcagcctc agggagccgg ataaaacccg ttaaaagtgg cttgcctata
     3961 ctgctgcgcg gcgctgacgc tttagaggcc ccaggattta ggcaaatcaa tgtagttagc
     4021 tcagtagcag gtcatatctg ggctataaat ggcattacgt gacactgaaa gagcttccca
     4081 gtggcatcgt atttggtgag ccccaaccta caagacgggc ggggatagcc gtgattggca
     4141 cccacagcaa ggcgataaag atgttcaacg gagcggctca gcaggtatgg ggatggtagg
     4201 atttagtctt catatgcctg tggaattaat caaccctcag agcccgacac acccggcatt
     4261 gcttacggta taagactgat tgagaaggtg tttgacgttt ttgtatccca gtctgtcggt
     4321 aaagtaaaca cagtgatcag taactggtag tactggaagt gattgcggtc gtaatgctga
     4381 ttttgcacgc tacgacaggt atattctgtt tggtatctgt agaggttgtg atggcgctga
     4441 ccttgcacat gccaggtacg ttagacttaa taataaaggg agtattagca ggagggattt
     4501 tttcggactc agttgta
//
LOCUS       DACADF010000240         4494 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.242, whole genome shotgun sequence.
ACCESSION   DACADF010000240 DACADF010000000
VERSION     DACADF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.242"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            134..1291
                     /gene="acrE"
                     /locus_tag="ISB30_004610"
     CDS             134..1291
                     /gene="acrE"
                     /locus_tag="ISB30_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HAP1992164.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1303..4407
                     /gene="acrF"
                     /locus_tag="ISB30_004611"
     CDS             1303..4407
                     /gene="acrF"
                     /locus_tag="ISB30_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HAP1992165.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIQEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPREATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         1093 a         1096 c         1183 g         1122 t
ORIGIN      
        1 gccagtattc ataactattc cttacatcga cgaatgataa tttgtaggat agcgaactgt
       61 atttttcttt ctgcgagtta acgcattgcc tttttgggca aataacgcgc ttttggtttt
      121 ttgaggaata gtaatgacga aacatgccag gtttttcctc ctgccctcct ttattctgat
      181 ctccgcggct ttaatcgccg gttgtaacga taagggagaa gagaaagctc acgtcggtga
      241 accgcaggtt accgttcata ttgtgaaaac ggccccgtta gaagttaaga ctgaattacc
      301 aggccgcacc aatgcttacc gtatagccga agttcgccca caggttagcg ggatcgtact
      361 gaatcgcaat ttcactgaag gcagcgatgt gcaagcaggc cagtccctgt accagatcga
      421 tcccgcgacc tatcaggcaa gttatgacag cgcgaaaggc gaactggcga aaagtgaagc
      481 cgccgccgcc atcgcgcatt tgacggtaaa acgttacgtt ccgctcgtgg gtacgaaata
      541 catcagtcag caggagtacg accaggccat tgctgatgct cgccaggccg atgccgccgt
      601 gattgccgca aaagccacag tcgaaagcgc tcgcatcaat ctcgcttata ccaaagtgac
      661 tgcgccaatt agcggacgta tcggcaaatc gaccgtgacc gaaggcgctc ttgtcactaa
      721 tgggcaaacg actgaactgg cgactgtcca gcagctcgat cctatctacg ttgatgtgac
      781 ccaatccagc aacgatttta tgaggctgaa gcaatccgta gagcaaggaa atttgcataa
      841 ggaaaacgcc accagcaacg tagagttggt catggagaac ggtcaaacct atcccctgaa
      901 aggtacgctg caattctccg atgtgaccgt tgatgaaagc accggctcca taaccctacg
      961 tgctgtcttc cctaacccgc aacatacgct tttgccgggt atgtttgtgc gcgcacggat
     1021 tgatgaaggc gtccaacctg acgccattct tatcccgcaa caaggcgtta gccgcacacc
     1081 acgtggtgat gcaaccgtgc tgattgttaa caataaaagt caggttgaag cgcgccctgt
     1141 cgttgccagt caggcgattg gcgataaatg gttgattagc gaaggtctga aatctggcga
     1201 tcaagtcatt gtcagcggcc tgcaaaaagc gcgtccggga gagcaggtta aagccactac
     1261 cgataccccc gcagatactg catcgaagta aggcaatctg acatggcaaa cttttttatt
     1321 cgacgaccga tatttgcatg ggtgctggcc attattctga tgatggcggg cgcactggcg
     1381 atcctacaat tgcccgtcgc ccagtatcca accattgcgc cgcctgcggt ttctgtttcg
     1441 gcaaactatc cgggtgctga tgcgcagacc gtgcaggata cagtgacgca ggttatcgaa
     1501 cagaatatga acggtatcga taacctgatg tatatgtcct ccaccagcga ttccgccggt
     1561 agcgtgacaa ttacccttac cttccagtcc gggaccgatc ctgatatcgc gcaagtacag
     1621 gtgcagaaca aactccagct cgccacgccg ttgctgccgc aggaggttca gcagcagggg
     1681 atcagtgttg aaaagtccag tagcagctat ttgatggtgg cgggctttgt ctctgataac
     1741 cctggcacca cacaggacga tatctcggac tatgtggcct ctaacgttaa agatacgctt
     1801 agccgtctga atggcgtcgg tgacgtacag cttttcggcg cacagtatgc gatgcgtatc
     1861 tggctggacg ccgatctgct aaacaaatat aaactgacac cggttgatgt gattaaccag
     1921 ttgaaggtac agaacgatca gatcgctgcc ggacagttgg gcggaacgcc agcgttacca
     1981 gggcaacagt tgaacgcctc gattattgct cagacgcggt taaaaaatcc ggaagaattc
     2041 ggcaaagtga ccctgcgcgt aaacagtgac ggctcggtgg tgcgtctgaa agatgtcgca
     2101 cgggttgaac ttggcggtga aaactataac gttatcgctc gcatcaacgg aaaaccggcg
     2161 gcgggcctgg ggattaagct ggcaaccggc gcgaatgctc tcgataccgc gaaagccatt
     2221 aaggcaaaac tggcggaatt acagccattc ttcccgcagg gaatgaaggt tctttaccct
     2281 tatgacacca cgcctttcgt ccagctttct atccaggaag tggtgaaaac gctgttcgaa
     2341 gccattatgc tggtgttcct ggtgatgtat ctgttcttgc agaacatgcg ggcaacattg
     2401 atacccacca ttgctgtacc tgtcgtgttg ttagggacat ttgccatact cgccgctttt
     2461 ggttactcca tcaatacgct aacgatgttt gggatggtac ttgccatcgg acttcttgtt
     2521 gatgatgcga tagtcgttgt ggagaacgtc gagcgcgtaa tgatggagga taaactcccc
     2581 cccagagaag cgacggaaaa atcgatgtcg caaatccagg gcgcgctggt aggtatcgcg
     2641 atggtactgt cggcggtatt tattccgatg gcattcttcg gtggctcaac aggggcaatt
     2701 tatcgccagt tctctatcac tattgtttca gcaatggcgc tttcagtact ggttgcgctg
     2761 attctcaccc ccgcgttatg tgcaacattg ctgaaaccag tctctgccga gcatcacgaa
     2821 aataagggag gattcttcgg ctggtttaac accactttcg atcatagcgt taatcactac
     2881 accaatagcg ttggcaaaat cctcggttcc acagggcgat atttactgat ctatgcgctg
     2941 attgttgcag gaatggtggt gttgttttta cgtcttccgt cttccttctt acctgaagag
     3001 gatcagggtg tctttctgac catgattcag ttacccgctg gcgcgacgca agagcggacg
     3061 caaaaagtgt tggatcaagt gacggattac tatctgaaga acgagaaagc gaacgttgaa
     3121 agtgtcttta cagttaacgg ctttagcttc agcggccagg cacaaaacgc cggtatggcc
     3181 ttcgtcagtc tgaaaccgtg ggaagagcgt agtggtgacg aaaacagtgc ggaagcagta
     3241 atccatcgtg ccaaaatgga attgggcaag atccgcgacg gttttgtcat tccatttaat
     3301 atgccagcca ttgttgaact aggcacggca acgggtttcg actttgagtt aattgatcag
     3361 gctgggctgg gtcacgatgc cctgacccag gcccgtaacc agttgcttgg catggcggcg
     3421 caacatcctg ccagcttagt cagcgtgcgc cctaatggcc tggaagacac cgcgcagttt
     3481 aaactggaag ttgaccagga aaaggcgcag gcattaggtg tttcactttc tgacatcaat
     3541 cagaccattt caacggcgtt gggtgggact tacgttaacg acttcatcga ccgtggtcgc
     3601 gtgaaaaagg tgtatgttca ggcggatgcc aaattccgta tgctgccaga agatgtcgat
     3661 aaactttatg tccgcagcgc caacggcgaa atggtgccat tctctgcctt taccacttca
     3721 cattgggtgt atggctctcc gcgactggaa cgctacaacg gtctgccgtc aatggagatt
     3781 cagggggaag ccgcgccagg aaccagttcc ggcgatgcta tggcgttgat ggaaaacctt
     3841 gcgtcaaaat taccggcggg cattggttat gactggacgg gtatgtcata tcaggaacgt
     3901 ttatcgggaa accaggctcc cgctctggta gcaatttcct ttgtggttgt tttcctgtgc
     3961 cttgctgcac tctatgaaag ctggtcaatt cctgtctcgg ttatgttggt cgtgccgtta
     4021 gggattgtcg gcgtgctact ggcggcaaca ctctttaatc aaaaaaatga cgtctacttt
     4081 atggtgggct tgctaacgac aattggcttg tcggccaaaa acgctatttt gattgttgag
     4141 ttcgctaaag atctcatgga gaaagagggt aaaggtgttg ttgaagcgac actaatggca
     4201 gtacgtatgc gcctgcgtcc tatcctgatg acctctctcg cctttattct cggcgtatta
     4261 ccgctagcta tcagtaacgg tgccggcagt ggcgcgcaga acgcagtggg tatcggggta
     4321 atgggcggga tggtttctgc aacgttgctg gcaatcttct tcgtgccggt gttctttgtg
     4381 gtgatccgcc gttgctttaa aggataaatc agaaacataa aggcactttc gggtgccttt
     4441 attatttcca gtgaaaccca taaaaattaa taagatattc ctctactcac tttt
//
LOCUS       DACADF010000241         4479 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.243, whole genome shotgun sequence.
ACCESSION   DACADF010000241 DACADF010000000
VERSION     DACADF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.243"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            147..1397
                     /gene="frc"
                     /locus_tag="ISB30_004612"
     CDS             147..1397
                     /gene="frc"
                     /locus_tag="ISB30_004612"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAP1992166.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            1451..3145
                     /gene="oxc"
                     /locus_tag="ISB30_004613"
     CDS             1451..3145
                     /gene="oxc"
                     /locus_tag="ISB30_004613"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAP1992167.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            3215..4159
                     /gene="yfdV"
                     /locus_tag="ISB30_004614"
     CDS             3215..4159
                     /gene="yfdV"
                     /locus_tag="ISB30_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAP1992168.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            4233..>4479
                     /locus_tag="ISB30_004615"
     CDS             4233..>4479
                     /locus_tag="ISB30_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAP1992169.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDH"
BASE COUNT         1172 a          950 c         1083 g         1274 t
ORIGIN      
        1 gcgatgaaat gattttcagc acttgataca ggaatttaaa aatatcgagt gcagctacga
       61 cgatattttg cagattattt atagaggcgt tatgccttaa ataaacgcta aattaacaca
      121 tatttaatta actaataggt attgctatgt caactccact tcaaggaatt aaagttctcg
      181 atttcaccgg tgtgcaatct ggcccatctt gtactcaaat gctggcctgg tttggcgctg
      241 acgtcattaa aattgaacgt cccggcgttg gtgacgtaac gcgccaccag ctgcgagata
      301 ttcctgatat cgatgcgctt tacttcacca tgcttaacag taacaaacgt tctattgaat
      361 taaataccaa aacagcggaa ggcaaagagg taatggaaaa gctgatccgc gaagctgata
      421 tcttagtcga gaactttcat ccaggggcca ttgatcatat gggcttcacc tgggagcata
      481 ttcaagaaat caatccacgt ctgatttttg gttcgatcaa agggtttgat gagtgttcgc
      541 cttatgtgaa tgtaaaagcc tatgaaaacg ttgctcaggc agcgggtggc gcggcatcta
      601 ctacaggttt ttgggacggt ccgccgctgg taagcgctgc agcgttgggt gacagcaaca
      661 ccggaatgca tttgctgatc ggtttacttg ctgctttgct gcatcgcgaa aaaacggggc
      721 gtgggcaacg agtgaccatg tcaatgcagg atgccgtgtt gaacctttgc cgcgtgaaat
      781 tacgcgacca gcagcgtctc gataaattgg gttatctgga agaatacccg cagtatccga
      841 atggcacatt tggtgatgca gttccccgcg gtggtaatgc gggtggtggt ggtcagcctg
      901 gctggatcct gaaatgtaaa ggctgggaaa ccgatcctaa cgcctatatt tatttcacta
      961 ttcaggagca aaactgggaa aacacctgta aagccatcgg caaaccagaa tggattaccg
     1021 atccggcata cagtacagcc catgcccgac agccacatat tttcgatatt tttgctgaaa
     1081 tcgaaaaata cactgtcact attgataaac atgaagctgt ggcctatttg actcagtttg
     1141 atattccttg cgcaccggtt ttaagtatga aagaaatttc acttgatccc tctttgcgcc
     1201 aaagtggcag tgttgtcgaa gtggaacaac cgttgcgtgg aaaatatctg acagttggtt
     1261 gtccaatgaa attctctgcc tttacgccgg atattaaagc tgcgccgcta ttaggtgaac
     1321 ataccgctgc tgtattgcag gagctgggtt atagcgacga tgaaattgct gcaatgaagc
     1381 aaaaccacgc catctgatat taacgggggc ttctggctcc caatttataa aaatttcgag
     1441 gttattaatc atgtcagatc aacttcaaat gacagatggt atgcatatca tcgttgaagc
     1501 attaaaacag aataatattg acactattta tggtgttgta ggtattcctg tgacggatat
     1561 ggcgcgccat gcccaggcgg aaggcattcg ttatattggt tttcgtcatg agcagtcggc
     1621 aggttatgcc gctgcagcaa gcggttttct tacccaaaaa ccagggatct gcctgacagt
     1681 gtctgcgcca ggattcctca acggtttgac cgcattggcc aatgcaacgg taaatggttt
     1741 tccgatgatc atgattagcg gctccagcga ccgcgcgatc gtcgatctcc agcaaggtga
     1801 ttatgaagag ctggaccaaa tgaatgcggc aaaaccgtat gccaaagcag catttcgcgt
     1861 taatcagccg caggatcttg gcattgcatt ggcacgcgct atccgggtct cagtatcggg
     1921 tcgtcctggc ggtgtttatc ttgatttgcc agcaaatgtc ctggccgcga cgatggaaaa
     1981 agacgaagcg ttaaccacga ttgttaaagt tgaaaatccg tcgccagcat tattgccatg
     2041 cccgaagtca gtcactagcg caatttcgct tttagcaaaa gctgaacgac cattaattat
     2101 tcttggcaaa ggcgcggcgt attcacaatc tgatgaacag cttcgtgaat ttattgaaag
     2161 tgcccagatt ccattcctgc caatgtctat ggcgaaaggg atcctcgaag atacgcatcc
     2221 actctctgcg gcagctgcgc gttcgtttgc cctggcaaat gctgacgttg tcatgcttgt
     2281 tggtgcacga ctgaattggt tactggcaca cggtaaaaaa ggatgggcgg cagatacaca
     2341 gtttattcaa ctggatattg aaccgcagga aattgacagc aaccgcccca ttgctgtgcc
     2401 agtcgttggc gatattgcat ccagtatgca aggtatgctg gcagaattga aacaaaacac
     2461 atttacgact ccactggtgt ggcgtgatat tttaaatatc cacaagcagc aaaatgcaca
     2521 aaaaatgcat gaaaagttaa gtacagatac ccaaccatta aattacttta atgcattgag
     2581 tgccgtgcgc gatgttttgc gcgagaacca ggatatttat ttagttaatg aaggggcaaa
     2641 taccctggat aatgcacgaa atattattga tatgtataaa ccacgtcgtc gtctggattg
     2701 tggcacctgg ggtgtcatgg gcatcggtat gggctatgcc atcggtgcta gcgtgacctc
     2761 tggttctccg gttgtcgcta ttgaaggtga tagtgctttt ggtttcagtg ggatggagat
     2821 tgaaacgatt tgtcgatata acctgccggt gacgatcgtt atttttaata atggcggcat
     2881 ctacagagga gacggtgttg acctcagcgg cgctggtgca ccatcgccaa cggatctgtt
     2941 gcaccatgca aggtatgaca aattaatgga tgcgtttcgt ggcgttggct ataacgtcac
     3001 cacgacagat gaacttcgtc atgctttaac caccggaatt cagtcgcgca aaccgaccat
     3061 tattaatgtg gtcatcgacc cagcagcagg aactgaaagt ggccatatta ccaaacttaa
     3121 cccaaaacaa gtcgctggta attaaatact gacatcgtct cctcttgcca acttattatg
     3181 cgatggtgag gggaccctta tcggaaatac tattatgcta acatttttta ttggcgattt
     3241 attgcctatt atcgtaatca tgctgttggg ttattttagc ggcagacgag aaactttttc
     3301 agaagatcaa gcccgggcat ttaataaact ggtattaaac tacgctcttc ctgcggctct
     3361 atttgtttct attaccaggg caaacaggga aatgattttt gcggacactc gcctgaccct
     3421 tgtatcactt gtggttattg tcggatgttt ctttttctcc tggttcggtt gctacaaatt
     3481 ttttaaacgt acccatgcag aagcagcagt atgtgcatta attgcaggtt cacctaccat
     3541 tggattcctg gggtttgcag ttctcgatcc tatttatggt gattccgtat caacaggttt
     3601 agtggtagca attatttcta ttattgttaa cgcaattact attcctattg gtctgtattt
     3661 gctgaatcct tcctcaggag cggatggtaa gaagaatagt aatctgagcg cattaatttc
     3721 tgcggcaaag gagccagtag tatgggcacc tgttctggca acgatcctgg tgctggttgg
     3781 ggtaaaaatt ccggcagcat gggacccaac ctttaatctg attgcgaagg ctaactcagg
     3841 ggtagcggta ttcgctgcgg ggttgactct ggctgcacat aaattcgagt tcagtgccga
     3901 aattgcttat aacactttcc tgaagctgat tctgatgcca ctggcgctgc ttctcgtcgg
     3961 tatggcatgt catttgaata gcgaacattt gcagatgatg gtactggcag gcgcattacc
     4021 gccggcattc tccggaatca ttattgccag ccggtttaat gtctacaccc gtactggtac
     4081 agcgtcattg gctgtgagcg tactgggttt tgtcgtcacg gctcctttgt ggatttatgt
     4141 cagtcgactg gtttcataat ttatcatgtt attgatttta cataaatgcc gaagtgtctt
     4201 ttcaccttcg gcttttttca aggcataagc aaatgacaaa taatgaatgc aaagggccgt
     4261 ttgaaggctt attagttatc gatatgacgc atgtccttaa tgggcctttc ggaactcaac
     4321 ttctttgtaa tatgggcgca agggtaatta aagttgagcc accgggtcat ggtgatgata
     4381 cccgcacatt tggtccctat gtggatggac agtcactcta ttacagtttt attaatcatg
     4441 gcaaagagag tgtggttctt gatttaaaga atgatcacg
//
LOCUS       DACADF010000242         4461 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.244, whole genome shotgun sequence.
ACCESSION   DACADF010000242 DACADF010000000
VERSION     DACADF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.244"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..514
                     /locus_tag="ISB30_004616"
     CDS             <1..514
                     /locus_tag="ISB30_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1992170.1"
                     /translation="EPCLSRKSSEGKPQIFTTLRNIDLTLLTILKLHMSIKESLMQRK
                     CLICPPSAIYQPIQKLRVIFPDLLFIRKGQGVTPTAFAMYLYESITQGLESILGALDI
                     EGSYDKQRTITIAPSPSVGALVLPVIYRAIKTHYPQLLLRNPPISGAENQLSQFQTDL
                     IINNMFCTGS"
     gene            746..1711
                     /gene="lipA"
                     /locus_tag="ISB30_004617"
     CDS             746..1711
                     /gene="lipA"
                     /locus_tag="ISB30_004617"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAP1992171.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1811..2014)
                     /gene="tatE"
                     /locus_tag="ISB30_004618"
     CDS             complement(1811..2014)
                     /gene="tatE"
                     /locus_tag="ISB30_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAP1992172.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2143..2931)
                     /locus_tag="ISB30_004619"
     CDS             complement(2143..2931)
                     /locus_tag="ISB30_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAP1992173.1"
                     /translation="MLVAAGQFAVTSVWEKNVEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3024..3407
                     /gene="crcB"
                     /locus_tag="ISB30_004620"
     CDS             3024..3407
                     /gene="crcB"
                     /locus_tag="ISB30_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAP1992174.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3461..3670)
                     /gene="cspE"
                     /locus_tag="ISB30_004621"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3461..3670)
                     /gene="cspE"
                     /locus_tag="ISB30_004621"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAP1992175.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3845..4405)
                     /locus_tag="ISB30_004622"
     CDS             complement(3845..4405)
                     /locus_tag="ISB30_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAP1992176.1"
                     /translation="MNVSKYVAIFFFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     ATFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1180 a         1143 c          989 g         1149 t
ORIGIN      
        1 tgaaccctgt ttaagcagaa aatcgtcaga aggtaaacca caaatattta caaccttacg
       61 aaatattgat cttacccttc tgactatttt gaagttgcat atgtccataa aggaatcgtt
      121 aatgcagcga aagtgcttaa tctgcccccc ctcggcaatc tatcagccta ttcagaaact
      181 gcgcgttata ttccctgacc tattgtttat tcgcaaaggc cagggtgtca ctcctaccgc
      241 atttgcgatg tatctatatg agtctatcac tcagggactt gagtccattc ttggcgcgct
      301 ggatatcgaa ggaagctatg ataagcaacg gacgataact attgcgccct ccccctcggt
      361 cggagccctg gtccttcctg tcatctatcg ggcgattaaa actcactatc cacagctttt
      421 actgcgcaac ccgcccatca gcggcgcgga aaaccaactc agtcagtttc aaaccgatct
      481 catcatcaat aacatgtttt gcacggggtc atagatgtta tccgtaatgc attttgaaaa
      541 agtattgtta cagccagatc acagcgcaca acgataaaat ggcacaatcc aacaacaatt
      601 ttacattttc gcgaccgctt tggctgcttt caggtccgtt tcaatgatat actgccagtc
      661 gttaattcaa aaatagttga taattacaac aatctattga attgaaacgc tttccttcgt
      721 aattcgcaac tggaacacgc acgctatgag taaacccatt gtgatggaac gcggtgttaa
      781 ataccgcgat gccgataaga tggcccttat cccggttaaa aacgtggcaa cagagcgcga
      841 agccctgctg cgcaagccgg aatggatgaa aatcaagctt ccagcggact ctacacgtat
      901 ccagggcatc aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc
      961 ctgccctaac ctggcggaat gctttaacca cggcactgca acgtttatga tcctcggcgc
     1021 tatttgtacc cgccgttgcc cgttctgtga cgttgcccac ggtcgcccgg tagctcctga
     1081 tgccaatgaa ccagtgaaac tggcgcagac cattgccgat atggcgctgc gttatgtggt
     1141 tatcacctcc gttgaccgtg atgatctgcg cgatggcggt gcccagcact ttgcggattg
     1201 cattactgcc attcgggaaa aaagcccgca aatcaaaatt gaaactctgg tgccggattt
     1261 ccgcggtcgt atggatcgtg ctctggatat tctgactgca acgccaccag atgtgttcaa
     1321 ccataacctg gaaaacgtac cgcgtattta ccgtcaggta cggcctggtg cagattacaa
     1381 ctggtcgctt aagctgctgg aacgctttaa agaagcgcat ccggaaatcc cgaccaagtc
     1441 tggtctgatg gtgggactgg gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct
     1501 gcgccgtcat ggtgtgacga tgctaacgct ggggcaatat ttgcagccaa gccgccatca
     1561 cctgccggtt caacgttacg ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct
     1621 ggcgatgggc tttacccatg ctgcatgcgg tccgtttgtc cgctcttctt accacgccga
     1681 tttgcaggcg aaagggatgg aagttaagta atcctgtaat aattacttac atctgtctga
     1741 taaaaaaccc gctttttgaa gcgggttttt gtatcgaaca agatattgag ggagcgtcct
     1801 gctcgccacg tcactcttta tgagagagct tttcagcctg aagatcaacg tctgcgcctt
     1861 ttttcgccgc agcatcgtca tcattcatcg ccttcttgaa ccctttaatg gccgctccaa
     1921 ggtctccgcc cagcgtacgt aacttcttag tcccaaacag cagaacgacc agcgccgcaa
     1981 ctaccagcag tttggtaata ctaatctcac ccatagatac cttcttgaca tataaccggc
     2041 aacagaacgc gccttaaaaa ccagagaata ttaacggtca ttcgacgcgc gcacacaata
     2101 gcaatctgta acaaggtgaa tcaagcgccg agtaaaaaaa catcataata attgcggcgg
     2161 cgcaaagcga cggttgttta agacgggcag ttgcgcgcgc acctgacgca cacgttcggg
     2221 cgtcacttcc gccataatga gtgcaggcat ttctgacgct gccgcaatgg tgacgccaaa
     2281 gggatctata atccggcttt gcccgatatt tttgttcccg cactcccccg ccgccaccat
     2341 ataacaggtg gtatccagcg cacgagcggc aagcaacgtt gaccagtgat gctctttgag
     2401 cggcccacga acccaggcgg caggaagtac cagtatttca gctccctgta atgcctgtgc
     2461 cagcgccagc tctggaaagc gtaagtcata acaggtcatc agaccgacct tcatcccctc
     2521 cacctccagt aacggagcta tttcattacc agcatcaaca tggcgtgatt cctgaatggc
     2581 aaatgcatca tagagatgca gtttggcata acgggcgacg atgtttcctg cctgtagcgc
     2641 caccagcata ttccatgcgc gccccggtgt tgaaggaaca tgaatcgtca gaattgtcgt
     2701 catcatgtta cgtttacttt ctcgccgtaa acgaccgagg aattcgcctt ccagcagttg
     2761 tgctgattta accgatagat ctgcatcatg atcatcacgc gccagtaatg cttccggcag
     2821 gacaaacagc gaaacctcgt tttccgccgc ctgcgccatc aacgaggcac aaatctcaac
     2881 gttcttttcc cacacagatg taacagcaaa ctgtcctgcg gcaaccagca ttgccacctc
     2941 ctgtcagagt cgtaaaattc gccaacggcg attattcggc gttacactta tcactcatac
     3001 aaatcaaata gcaggatttt gcagtgttac aacttctttt agcagttttt attggcggtg
     3061 gtacgggaag cgtggcgaga tggctgttaa gtatgcgatt taacccgctg catcaggcga
     3121 ttccgttggg aacgctggca gcaaacctga ttggggcatt catcatagga atgggattcg
     3181 cctggttcag caggatgacg aacattgatc cagtgtggaa agtattaatc accaccggat
     3241 tttgtggcgg tctaacaacc ttctcaacat tttcggcaga agtggtgttt ttgttacaag
     3301 agggccgctt tggctgggcg ttactgaacg ttttcgtcaa ccttttgggg tcttttgcca
     3361 tgaccgcact ggctttctgg ctgttttccg cctcaaccgc acactaaaga ccaaaaaaaa
     3421 cccgctgatt aagcgggttt tgaattcttg ctgacgtatc ttacagagcg attacgtttg
     3481 cagcagaagg gcctttggca ccgttagtga tttcgaactc tacgcgctga ccttcagcaa
     3541 gagttttaaa accattagtc tggattgcag agaagtgtac gaacacgtct ttgctgccgt
     3601 cttccggagt aatgaaaccg aatcctttgg actcattaaa ccacttaacg ttacctttaa
     3661 tcttagacat caaaattacc tttacatgaa aaatagacac aaatgctgtg tcggttacca
     3721 gtacaccaat tgtggtacgc tttgtccagt gcaactttac taaaaagtga taaaagtcgc
     3781 gttatttttg atgttaatta ttttagtggt tgattttaaa gaatttacta aaacttcatt
     3841 tgtctcaaaa ctgaaagcgc atccaggcaa agtacacatt gccattgttg taggtacccg
     3901 gaatgtaggt catctgaaac gtcgcggggc cataccccac agaggccaat ggcagtagaa
     3961 ccggaagagg gatgtaattc cagttatcgc gcgccgttac gccagcggta aatcccaggc
     4021 ctaaatgaaa attttcatcc gccagcggtc gccaggtact ttcccatccg tatccggcaa
     4081 tcggttccca tttgttccac gagtccttaa atgccattgc atacaggcca tgccagtttc
     4141 ctttttcatc ccaacgcgac tgaccaaaac cgccacccca cggtcgctcg ttatagcgat
     4201 cggttttttc tttgtcatag gcaaaacgcg catgccaggt gatggcagga atatataaat
     4261 cataatgttc tggctgttgc caggtttgtg caatattttc tctaaacgtt gtcatccact
     4321 catcagcgtt agcaaaaact ttaccaacac tgattaactg aataaaaaca aagaaaaata
     4381 tagcgacata tttactcacg ttcatttgtg accattaaac atttatcaaa aatctactac
     4441 taacatagca aaactacaat t
//
LOCUS       DACADF010000243         4441 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.245, whole genome shotgun sequence.
ACCESSION   DACADF010000243 DACADF010000000
VERSION     DACADF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.245"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..208
                     /locus_tag="ISB30_004623"
     CDS             <1..208
                     /locus_tag="ISB30_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAP1992177.1"
                     /translation="GYGVLWALMVLLMNMHDVSESVAGLAIALLTLMVCSSAGERVKR
                     FWPRQFFVMFYAVHLAVLGIVVSM"
     gene            280..873
                     /locus_tag="ISB30_004624"
     CDS             280..873
                     /locus_tag="ISB30_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAP1992178.1"
                     /translation="MAEEKRPVLSLKRKPAENSTAPAEATPAPGVVRRKKVVVVSSPP
                     AWKAKKAKLEKAKQAAEADARNAAPAPDKAVKTPPPVRYLRLLPPEQAIMTLKAFWPQ
                     LFDGNSPRLLARGMREQLFADIVNRDLPLSHKQVIKCLKSLTRSADYLSRMKVGASRY
                     DLQGNAVATVTAEEAQYASERMMKELLRAERKMSQTL"
     gene            1034..1636
                     /locus_tag="ISB30_004625"
     CDS             1034..1636
                     /locus_tag="ISB30_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992179.1"
                     /translation="MTMTEKIIALVDYENIGTLENVRLSRYERLILFTGPQQEFIRFP
                     AVTYAGDISVSVFQVSNVSKNNVDFHLVFELGRLSATAPEETIFHIISNDKGYDGVIA
                     ELCRAGRRCCRISSPCSAEKPKAAPMVISNDEVLHLTDKVQSLSKKSAGNRPANTSSL
                     MNYIKSLSGNTKGMALYGRVKEELIRRGVIAVYEKTVVWR"
     gene            1686..2330
                     /locus_tag="ISB30_004626"
     CDS             1686..2330
                     /locus_tag="ISB30_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992180.1"
                     /translation="MFRYFILRPEQQLFCYLYGCALALVQMVLFSPVSRASGFYLVAL
                     SVALFWAGLALYTRHIDRMRKPEVSPLVSIRDGIQVVAEVPRYEKARLEWEILRDDEV
                     FRQQRCELTGLTGRVISRGLLYTPAVMLVGIGILAWGSPQDAIRLINALRNMPAAELV
                     HQIGFVLCHFLQISVISVLIADVVAGRGLPNVFRRALLDRLPAEFCLIRRGTER"
     gene            2386..3036
                     /locus_tag="ISB30_004627"
     CDS             2386..3036
                     /locus_tag="ISB30_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAP1992181.1"
                     /translation="MAKILMMAGSTVVVLSLLGFLVLLLKGRAVTKTSPVLRSLKALG
                     IRPSEAEQRLCRQRVWVGNDTLMTPREQHFFRALLRHTSRTRWLLCPQVRVADIATLS
                     PHIRPRSRTWWQLFRMASQWHCDVVIVDIHTFAIIGAVELDDASHQKKHRIRRDILLE
                     EVLRQAGIPLLRDRDSESLVRKVVEFLKYREAGAEEKSITVMKSTTKHSEHKEKEK"
     gene            3033..3341
                     /locus_tag="ISB30_004628"
     CDS             3033..3341
                     /locus_tag="ISB30_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992182.1"
                     /translation="MIIAAEVKRDEQGYWTHPALVRSGCETSAELSYWLRTHKLQCFV
                     MTMRDEAPEAFAAGFTDEAPDARGWIPEPPDDDGWFLGSVHDTGDGPVCYWFRHTTKS
                     "
     gene            complement(3574..4434)
                     /locus_tag="ISB30_004629"
     CDS             complement(3574..4434)
                     /locus_tag="ISB30_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAP1992183.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT         1094 a         1056 c         1210 g         1081 t
ORIGIN      
        1 gggatacggg gtgttgtggg ccctcatggt gttgctgatg aatatgcatg acgtgagtga
       61 atcagtggcc ggactggcga ttgcgctgct gacgctgatg gtgtgctcat ctgccggaga
      121 acgtgtgaaa cgcttctggc cgcggcagtt cttcgtgatg ttctatgccg tgcacctggc
      181 ggtactgggt attgtcgtct caatgtgaac gcaggagcag gaaagggtga taccgttcag
      241 gtatagtagc tcttattaac tgaacgatag gcaattgtta tggcagaaga aaaacgccct
      301 gtactgagcc tgaaacgaaa accggcggag aacagcacgg cccccgcaga ggcaacccct
      361 gctcctggtg tggtgcgccg aaagaaggtc gtggtggtct cgtccccgcc ggcctggaag
      421 gcaaagaaag cgaaacttga gaaggctaaa caagcggccg aagccgacgc gcgcaacgct
      481 gcgccggcgc cggataaagc ggtaaagacg ccacctccgg tgcgttatct tcgcctgctg
      541 ccgccggaac aggccatcat gaccctgaag gcattctggc cacagctgtt cgacggaaac
      601 tcaccccgtc tgctggcgag gggaatgcgc gagcagctgt ttgccgacat tgtgaaccgc
      661 gacctgccgc tgtcgcataa acaggtcatt aagtgcctga agagtctgac ccggtctgcg
      721 gactatctgt cccggatgaa ggtgggggcc tcccgctatg atctgcaggg gaatgccgtt
      781 gcgaccgtca ctgcagagga agcgcagtac gccagtgagc gcatgatgaa ggagctgctg
      841 cgagctgaac gaaagatgag tcagaccctt taattatatt tacccattcc tgataattat
      901 acctgggaat cctcagctaa tagatgtaac gaaaattagc tgagggtatg cctgaaatag
      961 caacaggtat tcattttata tttgccgcat tacaggctgc ggcatcataa ctgtccgtgc
     1021 agacagattc tggatgacga tgactgaaaa aattattgct ctcgtagatt atgaaaatat
     1081 tggtacactt gaaaatgtcc gtctgtcccg ttatgaacgc cttattttat ttaccgggcc
     1141 tcagcaggag tttatacgtt ttccagctgt aacgtatgca ggtgatatat cggtaagcgt
     1201 attccaggtt tcgaatgtgt caaaaaataa tgtggacttc catctggtgt ttgagctggg
     1261 aagacttagc gcgactgcac cggaagagac gatatttcat attatctcga atgataaagg
     1321 ctatgatggc gtcattgccg agctatgcag ggcggggaga cgatgttgcc gtatatcatc
     1381 gccttgctct gcagagaaac cgaaggcggc acctatggta atcagtaacg atgaagtgct
     1441 gcacctgacc gataaagtcc agtcattaag taaaaaatct gcagggaacc gcccggcgaa
     1501 tacttcatcc ctgatgaatt atattaaatc cctgtccggt aatacaaaag gcatggcgct
     1561 ttatggtcgc gttaaagagg aattaatcag gcgtggtgtg attgcggtgt atgagaaaac
     1621 agtggtctgg cgataatgaa attaagacgg ctgtgaatgt tatttttatc taaaaaggaa
     1681 tcaatatgtt cagatatttc attctgcgcc ctgagcagca gttattctgt tatttatacg
     1741 gctgcgcgct ggcactggtt cagatggttt tattttctcc tgtatcccgg gcaagcggat
     1801 tttatctggt tgcgttatct gtcgcgctgt tctgggccgg tctggccctg tacacccggc
     1861 acatcgaccg gatgagaaag ccagaggtct caccgctggt cagtattcgc gacggtattc
     1921 aggtggtggc cgaggtgccg cgctatgaaa aggcccggct ggaatgggaa atcctccggg
     1981 atgatgaggt gtttcgccag caaaggtgtg agcttacagg cctgaccgga cgggttatca
     2041 gccgggggct gttgtatacg ccggcggtga tgctggtcgg tatcggtatt ctcgcctggg
     2101 gttccccaca ggatgccatc cggctgatta atgctcttcg taatatgcct gctgcggaac
     2161 tggtgcatca gattggtttt gtcctgtgcc acttcctgca gattagcgtc atatcagttc
     2221 tgattgctga tgttgtagcc gggagaggac tgccgaatgt atttcgccgc gcgttgctcg
     2281 acaggctgcc ggcagagttc tgccttatca gaagagggac agaaagatga ggaagacgac
     2341 aggaaaacgc atcgggaaag aaaggaacat cagggaataa aatacatggc aaagatatta
     2401 atgatggcgg gcagtaccgt tgttgtgctg agtttgctgg gatttctggt gctgcttctg
     2461 aaaggacgcg cagtaacgaa gacttccccg gttctgaggt cactgaaagc cctgggtatt
     2521 cgcccctcag aggctgagca acggttgtgt cgccagcggg tctgggtggg taatgacaca
     2581 ctgatgacgc cacgggaaca acattttttc cgggcattgc tgaggcatac cagccgaaca
     2641 cgctggctgt tgtgtcctca ggtccgggtg gcagatattg ccacactctc tccccatatc
     2701 cggccacgat cccggacctg gtggcaactc ttccggatgg catcacagtg gcactgcgat
     2761 gtggtcatcg ttgacatcca taccttcgct atcatcggcg cagttgaact ggatgatgcc
     2821 tcacaccaga aaaaacaccg tatccggcgc gatattctgc tggaagaggt tctgaggcag
     2881 gccggcattc cactactacg cgatcgggat tcggagagtc tggtaagaaa ggtcgtagag
     2941 tttctgaaat accgggaagc cggtgctgaa gaaaaatcca taaccgttat gaaatcaacg
     3001 acaaagcata gcgaacacaa ggagaaagaa aaatgattat cgccgctgaa gtgaaacgtg
     3061 atgaacaggg gtactggaca catcctgcgc tggtacgaag tggctgtgaa acttccgcag
     3121 aattaagtta ctggctcaga acccataaac tccagtgctt tgtgatgaca atgcgcgatg
     3181 aagcgcctga agctttcgct gccggattta cggatgaagc cccggatgcc cgtggctgga
     3241 taccggagcc accggatgat gatggctggt ttctgggatc tgtccacgat accggcgacg
     3301 gacccgtctg ctactggttt cgtcatacca caaaatcctg acgcctccgc atcgcgtcgt
     3361 tgtccaaccc cgggaacggg gaaaagccag tttccccgct cccggcgttt cttaaacacc
     3421 cgttaagggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3481 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3541 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3601 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3661 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3721 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3781 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3841 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     3901 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3961 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4021 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4081 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4141 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     4201 taccgcacca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4261 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4321 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4381 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4441 c
//
LOCUS       DACADF010000244         4432 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.246, whole genome shotgun sequence.
ACCESSION   DACADF010000244 DACADF010000000
VERSION     DACADF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.246"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            5..2257
                     /locus_tag="ISB30_004630"
     CDS             5..2257
                     /locus_tag="ISB30_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016536327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAP1992184.1"
                     /translation="MKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVSVGK
                     TTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMSGFG
                     RVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEGINA
                     LAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGSIGV
                     MAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSARTY
                     ENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSAQNR
                     SDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPFAPS
                     GQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDDKEG
                     GFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAETWQA
                     GFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSLPEVIGNGWEGI
                     SDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTHFGA
                     GDITELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQDLP
                     QIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSARGR
                     TWIFGGDIRF"
     gene            2326..3501
                     /gene="senB"
                     /locus_tag="ISB30_004631"
     CDS             2326..3501
                     /gene="senB"
                     /locus_tag="ISB30_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HAP1992185.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(4009..4314)
                     /locus_tag="ISB30_004632"
                     /pseudo
     CDS             complement(4009..4314)
                     /locus_tag="ISB30_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT         1236 a         1061 c         1052 g         1083 t
ORIGIN      
        1 cgttataaaa ctggctatcg gctcaggcat attattgctc agctgcggtg cttactcaca
       61 atccatcagt gaaaaaacta attccgacaa aaaaggagcg gcagaattca gtccgctcag
      121 cgtttctgtc gggaagacga ccagtgagca ggaagctctc gagaaaacag gcgcgaccag
      181 ttcccggaca acggacaaaa acctgcaatc acttgacgca acagtgcgta gtatgcctgg
      241 tacttatact caaatagatc ctggtcaggg agcaatcagt gtgaatattc gaggcatgag
      301 cggatttggt cgtgtaaaca ctatggtcga tggtattacc cagagttttt acggaacctc
      361 tacctccgga acaacgacgc atggttcaac taacaatatg gctggcgtac ttatagatcc
      421 taacttactg gtagcagttg atgttacacg cggtgacagc agtggctctg aagggatcaa
      481 cgcccttgcc ggtagtgcaa atatgcgtac tattggcgtt gacgatgtaa tatttaacgg
      541 taatacatat ggccttcgtt cacgtttctc tgtcggtagt aatgggctgg gacgcagcgg
      601 aatgatcgcc cttggtggaa aaagcgacgc ttttacggat acgggaagca ttggcgttat
      661 ggctgctgtg agcggcagtt ctgtgtactc taatttctca aatggttctg gaattaacag
      721 caaagagttt ggttatgata aatatatgaa gcagaacccc aaatcccaac tgtataaaat
      781 ggatatcaga ccagacgaat ttaacagctt cgaactttcc gctcgaacct atgaaaataa
      841 atttacacgt cgtgatataa ccagtgacga ctattacatt aaatatcatt acaccccttt
      901 ttctgaatta attgacttta acgtaacggc cagtaccagt cgcggtaatc aaaagtatcg
      961 tgatggctcg ctgtatactt tctacaaaac ctcagcgcaa aatcgttctg acgcgctgga
     1021 tatcaacaat accagccggt tcactgtcgc ggacaatgac ctggagttta tgctgggcag
     1081 caaactgatg cgtacccgct atgaccggac cattcactca gcggcgggcg acccgaaagc
     1141 gaatcaggaa tcgatcgaga acaatccgtt cgcaccctcc ggccagcagg atatttcagc
     1201 gctgtatacc gggctgaagg ttacgcgcgg catctgggag gcagatttca atctcaacta
     1261 cacacgtaac aggatcacag ggtacaagcc cgcctgcgat tcacgcgtta tctgcgtgcc
     1321 acagggtagc tacgatattg acgataaaga gggtggcttc aacccttcag ttcagctttc
     1381 tgctcaggta acaccatggc ttcagccgtt cattggctac agcaaatcca tgcgcgcccc
     1441 gaacatccag gagatgttct tctctaattc aggaggcgca tccatgaacc cattcctgaa
     1501 gcctgaacgt gcagaaacct ggcaggcggg ttttaacatt gataccagag atttactggt
     1561 cgaacaggat gccctgcgct ttaaggctct ggcgtaccgc agcaggatcc agaactacat
     1621 ctacagcgag tcttatctgg tttgttctgg aggtcgtaaa tgcagtctgc ctgaggtgat
     1681 tggcaatggc tgggagggca ttagcgatga atacagcgac aatatgtaca tctacgttaa
     1741 ctcggcaagc gacgttatcg caaagggctt cgaactggag atggattatg atgcaggttt
     1801 tgcttttggc cgactctctt tcagccagca gcaaacagac cagccaacct ccatcgccag
     1861 cacccacttt ggcgcagggg atataaccga actgcccaga aaatacatga cgctggatac
     1921 tggtgttcgc ttcttcgata acgcgttgac cctgggcact atcataaaat acacaggcaa
     1981 ggctcgtcgc ctgtcgcctg attttgagca ggacgaacat accggcgcaa taatcaaaca
     2041 ggatttgccg cagatcccaa cgattatcga tctctatggt acttacgagt acaaccgcaa
     2101 cctgacactg aaactttcgg tacaaaacct gatgaacaga gattattcgg aggcgctgaa
     2161 taagctcaac atgatgccag gtcttggtga cgagacccac ccagccaatt ccgcgcgtgg
     2221 cagaacatgg atatttggcg gggacattcg tttctgatga caatttccgc taggttcaga
     2281 caatacgttt tctctcttat gtcaatttta ttgcaggaac gaaaaatgaa tattttcact
     2341 ttatccaaag caccgctata cctgttaatt tcactatttt tacccacgat ggccatggct
     2401 atcgatccac ctgaacgcga actttcgcga tttgccctga aaacgaatta ccttcagtcc
     2461 cctgatgaag gcgtctatga actggcgttt gataatgcca gtaaaaaggt gtttgcagca
     2521 gtcaccgatc gtgtaaatcg tgaagccaat aaaggctatc tgtattcgtt taattcagat
     2581 tcgctgaaag tcgaaaataa atacacgatg ccataccggg cattttcgct ggcgataaat
     2641 caggataaac atcagctcta tatcggacac acccagtcag cgtccctgcg tatcagtatg
     2701 tttgacaccc caaccggcaa actggtaaga accagcgaca ggttaagttt taaagcggca
     2761 aacgctgcag attcgcgttt tgagcatttt cgccatatgg tttacagcca ggattccgat
     2821 accctgtttg tgagttatag caatatgctg aaaacggccg agggcatgaa gcctctgcat
     2881 aagctgttaa tgctcgacgg gacgacgctt gccttaaaag gcgaggttaa ggatgcttac
     2941 aaaggtacag cgtatggtct gacgatggat gaaaaaacac agaaaatcta cgttggcgga
     3001 agagattaca tcaacgaaat tgatgcgaaa aatcagacgc tgctgcgtac catcccgttg
     3061 aaagatccga gaccacaaat cacaagtgtg cagaatctgg cggtggactc cgcttctgac
     3121 cgtgcctttg tggtggtatt cgaccatgac gatcgttccg gtacaaaaga tggactctat
     3181 atttttgact tacgcgacgg taaacagctt ggctatgtgc acacaggagc cggagctaac
     3241 gcggtgaaat acaatccgaa atataacgaa ctgtatgtca ccaacttcac tagcggcacc
     3301 atcagcgtag tggatgccac caaatacagc atcacccgtg aatttaacat gccggtctac
     3361 ccaaaccaga tggtgttgtc ggacgatatg gatacccttt acattggcat caaagaaggc
     3421 tttaaccgcg attgggatcc tgatgtgttt gtggaaggag ctaaagaacg tattctgagc
     3481 attgatttga aaaagtcgtg atcgggggga aaaaacatgg gataaaaact ccgttattcc
     3541 actaaactgg ccggactagg aaaagcaaac atctctgtcc tgccggtacg cctgttacgc
     3601 cgctccatga ggctgaggaa tctccagaaa acgagacagg cacagcattg tacgatcaac
     3661 tgatttgtgc caataattaa atgaaatcac agctatgcct gttcatgggg ttctaggccg
     3721 gaaccccaga tttttccgat tgtttgtttt ctaactgatt attcagtagt tttagagggc
     3781 tgacgctcag tggaacgaaa actcacgtta agaggttttg actatgataa aagtcaaagt
     3841 aggggtgagt ttggatatgg aaattattgt actataagga gagcatgaaa aaccctgggg
     3901 cagtcactga atgagggacc agcagcggta aaataaccag cataatttct gcatggtcga
     3961 tttttatacc gtgacaggct gttttttata cttgcaccga ttcccgtgtc aggccggcat
     4021 atacctcagc agactgatat cccgcgcgtt tttcttcagc cctgatacat tcagccccgc
     4081 aatggcccgg cggtatacca tccggccgcc gcacagcacg cattcgaacg ggtcacgact
     4141 caggaactgt ttcaccattt gtgcatagca cactttcgcc actgcttccg gtttatccat
     4201 ccccagagca cggtacacct gcggcagctt ctctccacac acccggttgg caaggaaccc
     4261 gaagtacctc accatcttaa aaaactgaac cgccccggga atcctggaga ctaaacttcc
     4321 tgagaaagag gtaaacagga tgactaaaaa tactcgtttt tcccccgaag tccgtcaacg
     4381 ggcagtccgt atggttctgg aaagtcaggg cgaatatgac tcacaatggg cg
//
LOCUS       DACADF010000245         4400 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.247, whole genome shotgun sequence.
ACCESSION   DACADF010000245 DACADF010000000
VERSION     DACADF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.247"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..658)
                     /locus_tag="ISB30_004633"
     CDS             complement(<1..658)
                     /locus_tag="ISB30_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP1992186.1"
                     /translation="MNNQTIRTSVPALDKTVRVCNYLFSSPGATFSQIQQDLDLPKSS
                     TSSLLNALVEHHLLRQERGRFFLGLKLYEWGNRSLEQFDISKVARPVLEKLRDSTDLI
                     CHLGILDEVAPIYILKLESTQPIGIRTWVGKKLPLHSSGVGKALCAWLPQDVIDTLLP
                     EETLPRFTQTTITRKSDLIQEFARIRERGWAFDNAEDSPGIYCIAAPVFNQTREVIAA
                     I"
     gene            898..1797
                     /locus_tag="ISB30_004634"
     CDS             898..1797
                     /locus_tag="ISB30_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HAP1992187.1"
                     /translation="MLTAFRGIFPPVPTIVDAQGKLDKAGMARMIDHVLENGANGMLI
                     LGSGGEFSQFSSQQRKEIAAFCLSHVDGKVPVLVGIACAGTAETIDLGHHAQEHGADG
                     VMVVNPYYAKLSDDARFMHYKSIADSLKIPVFLYNFPDLTGQDIGLEVITRLAREVPN
                     IVGIKDTIDNISHTREVINRVHAFRPDFIIFSGYDEYMLDTLLLGGHGGIPATFNFAP
                     NITRGIYQAVMENDLDKARYFQQQLAKLSPLYTLEQPFFGVIKTAIKLSGLDISTAVM
                     APALPLSDDKVARVKAILEHIHQ"
     gene            1814..3331
                     /locus_tag="ISB30_004635"
     CDS             1814..3331
                     /locus_tag="ISB30_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP1992188.1"
                     /translation="MNKTPILKLNNSDDVVIARTELPAGSWLESESMETRDLIPAGHK
                     IALRDIPAGSAVKRYGQIIGFATRDIRQGEHIHVHNLGMGSFERDYAWGIDSHLPEKD
                     VHQDTFMGIRRKDGRVATRNYIGVLSSVNCSATVVRAIEDHFRTRGLSDYPNVDGIVA
                     LPQSFGCAFGSDSEMMKVMRRTMAGYATHANFAGIVIVGLGCESFQIKDMMNAHGLHE
                     SPVFHTMTIQESGGTRNAIEKGISFILDMLPEANNVKREPVPVSEIILALECGGSDSY
                     SGISANPALGYAVDRLVRQGGTAILSETSEIYGAEHLLTRRAISREVGEKLIRRIKWW
                     EEYCKRTGSEMNNNPSAGNKAGGLTTVLEKSLGGIAKAGTTSLMDVYEYAEPVTAKGL
                     VFMDTPGYDPMAVTGQIAGGANLLCFTTGRGSAFGAKPTPCLKLSTNNALWQRQRDDM
                     DLNCGTIVDGEETIEQVGERIYQRIIELASGHRSRSEELGYGSQEFVPWIMNSIM"
     gene            3409..>4400
                     /locus_tag="ISB30_004636"
     CDS             3409..>4400
                     /locus_tag="ISB30_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAP1992189.1"
                     /translation="MERKPVLGIFLGEAAGIGPELVAKVVADGTVYKYCRPVLIGDAR
                     VLALGQKIAGVHFQWNVISDPTEADWTTGNVPLLDLKNFNPEKLTMGTIDTDSGYATG
                     ESLVTCMSLLKSGKIDGFVFAPLNKEAFKKGGWDIEDEHYLFAEQLGYLDKPRGLLNV
                     LGDLWVFRVTGHIPFKDIASHITPENVSRSIQLCYDTLRMAAVENPRIAVAALNPHAG
                     DGGTCGKEEIDVLIPVIEGFREKGMDMYGPVPGDTVFIHAFNHEYDAVVTLFHDQGQI
                     ATKLHSFDVGITVAAGLPFAITTPEHGTAFDIAGKGIAKTLATERAIAVAARMAS"
BASE COUNT         1213 a          932 c         1074 g         1181 t
ORIGIN      
        1 tgatagctgc aattacttca cgagtctgat taaataccgg agcagcaata caataaattc
       61 ccggtgaatc ctctgcgtta tcaaaagccc atccacgttc acgaatacgt gcaaattcct
      121 gtattagatc agatttacga gtgatagttg tttgcgtaaa tcgtggcaac gtttcctcag
      181 gcaacaatgt gtcaatgacg tcttgaggta accacgcaca taaagcctta ccaactccag
      241 aactatggag tggcaacttc ttgcctaccc acgttctgat gccaattggt tgtgtactct
      301 cgagtttcaa aatatagatt ggagcgactt catcgagaat gcccagatgg cagataagat
      361 cagtactgtc tctcagtttt tctagaaccg gcctcgctac cttactgatg tcaaactgct
      421 ccagagatcg attcccccat tcatacaatt tcagcccaag aaaaaaacgc cctctttcct
      481 gacgtaacag atgatgttcg acaagggcat tcagtaaaga tgatgtgctg ctttttggca
      541 gatccagatc ctgctggatc tggctgaagg tggcgcctgg agagctaaaa aggtaattgc
      601 agactctgac agttttatcg agtgctggta ccgatgttct gatggtttga ttattcataa
      661 aaaaatatga ttccttacaa tactgaatag tataccccat tcccccttga tgcgcagcgg
      721 cgcttagcac acatattata catggcctcc gcgtaccggc atgcctctat aaacctgact
      781 tatttcacac ttttcatatc gcattgtaaa aacatttgac atgcaaaacc tgaatttgat
      841 tcactttatt cagcataaag aacttgtgtt ccatatacag aacttagtga gagtgatatg
      901 ttgacagcgt ttagaggtat tttcccgcca gttcctacaa ttgttgacgc acaagggaaa
      961 ctagataagg ctggcatggc caggatgatc gaccatgttt tagaaaatgg tgcaaacggg
     1021 atgttgatcc ttggcagtgg gggagaattc tcacaatttt cttcccagca acgaaaagag
     1081 atagctgcat tttgcttaag tcatgtagat ggcaaagtgc ctgttctggt ggggatcgcc
     1141 tgcgcgggaa ctgcagaaac tattgattta ggacatcatg cacaggagca cggagctgat
     1201 ggtgtgatgg tggtgaatcc gtattatgca aaactcagcg atgatgcccg gtttatgcat
     1261 tacaaatcta ttgccgattc acttaaaatt ccagtgtttc tctataactt tcctgatctg
     1321 accggtcagg atattgggct tgaggtcatt acccggctgg cccgggaagt tccaaatatc
     1381 gtgggtatca aggataccat cgataatatc agccataccc gggaagtcat caaccgtgta
     1441 catgcttttc gtccggattt cattatattc agcggatatg acgaatatat gctggatacc
     1501 ctgttactgg gcggccatgg tggtattccg gcgacattca attttgctcc aaatatcaca
     1561 cgtggtattt atcaggccgt tatggaaaat gatcttgata aagcgcgtta ttttcagcaa
     1621 caactggcaa aactgtctcc gttatacaca ctggagcagc ctttctttgg cgtgataaaa
     1681 actgccatca aactgagtgg actggacata tctactgcag ttatggcacc ggctcttccg
     1741 ctcagtgatg acaaggtcgc cagggtgaaa gccattctgg aacacattca ccagtgatta
     1801 cgaggatcgt aagatgaata agactccgat actcaaatta aataactcgg atgatgtcgt
     1861 tattgccagg acagaacttc ctgccggaag ctggctggaa tctgaaagta tggaaacacg
     1921 tgaccttatt ccggcagggc acaaaattgc tcttcgggat attccggccg gcagtgccgt
     1981 taaacgctat ggacagataa tcggttttgc gacccgtgac atccgacagg gcgaacatat
     2041 tcatgtgcat aatctcggta tggggagttt tgagcgtgat tatgcctggg ggatagacag
     2101 tcatcttccg gaaaaggatg ttcatcagga tacctttatg gggatccgca gaaaagacgg
     2161 acgggtggca acccgtaatt atattggtgt actgagttct gtaaactgta gtgcaaccgt
     2221 agtaagggca attgaagatc atttccgtac ccgtggtctc agtgattacc ctaatgtgga
     2281 tggtattgtt gccctcccac aaagttttgg ttgtgccttt ggctctgaca gcgaaatgat
     2341 gaaagtcatg cggaggacaa tggcaggcta tgccacgcac gcgaactttg ccgggatcgt
     2401 gatcgtggga ctgggatgtg aatcattcca gattaaggac atgatgaatg cccacggatt
     2461 acatgaaagt cctgtattcc atacaatgac tattcaggaa agcggcggaa cacgtaacgc
     2521 tatagaaaaa ggtatttcct tcattctcga catgctcccg gaagccaaca atgtcaaacg
     2581 agaaccagtt ccggtatcag aaatcattct ggctcttgag tgtggcggct ctgatagcta
     2641 ttcaggaatt tccgctaatc cggctctcgg gtacgcagtt gatcgcctgg ttcgccaggg
     2701 aggaacggct attctgtcgg aaacatcgga aatttatgga gcagaacacc ttctgacacg
     2761 ccgtgccatt agtcgcgaag ttggcgagaa actaatccgc agaattaagt ggtgggaaga
     2821 gtactgtaaa cgtacaggga gtgaaatgaa taacaaccct tctgcaggta ataaagcggg
     2881 gggactgacc actgttctgg agaaatcgct gggagggata gcaaaagctg gtacaaccag
     2941 cttaatggat gtgtatgaat atgctgaacc ggtgacagca aaagggcttg tctttatgga
     3001 tacccccgga tatgacccga tggcagtaac cggacaaatc gccggaggag ccaacctgct
     3061 gtgttttacc acgggcaggg gatctgcctt tggcgcaaaa cccactcctt gtctgaaact
     3121 gtcaaccaat aacgcactat ggcaacggca gcgcgacgac atggatttaa actgtggcac
     3181 gattgttgat ggcgaagaaa caatagaaca ggttggagaa cgtatttatc agagaattat
     3241 tgagctggct tcaggtcatc gtagtcgaag tgaagagctg ggttatggga gccaggaatt
     3301 cgttccttgg ataatgaact caatcatgta ataactacgg gcataatttg ttttgcccgg
     3361 aaataaggaa acaaactgaa tcctgttttt gataattaga ggtgaataat ggaacgtaaa
     3421 ccagtactgg gcattttttt aggtgaagct gccggcattg gacctgaact ggttgccaaa
     3481 gtcgtagctg atggtactgt ctataaatat tgcagacctg ttctgattgg cgatgcccgg
     3541 gtacttgctc ttggacaaaa gattgctggt gtgcattttc agtggaatgt catcagtgat
     3601 cccactgaag ctgactggac tacgggaaat gtaccattac ttgatttgaa aaattttaat
     3661 cccgaaaaac taactatggg caccattgat accgactcag gttatgcaac aggagaatca
     3721 ctggtaacct gtatgtcatt acttaaatca ggtaaaattg acggttttgt cttcgctcca
     3781 ctcaataaag aagctttcaa gaaggggggc tgggatattg aggacgaaca ttatctcttt
     3841 gctgagcagt taggatattt ggataaacct cgtggcctgt tgaatgtcct tggtgacctc
     3901 tgggtttttc gcgtgacggg acatattcca ttcaaagata ttgccagcca tattacacca
     3961 gaaaatgtca gtcgttcgat tcagttatgc tatgacacat tacgtatggc ggccgttgaa
     4021 aatccacgaa tagcggttgc agcacttaat cctcatgccg gtgatggagg aacctgtggt
     4081 aaggaagaaa ttgatgttct cattccggtc attgaaggct tccgcgagaa agggatggat
     4141 atgtatggcc cagtaccggg tgatactgtt tttattcatg catttaatca tgaatatgat
     4201 gctgtcgtta ccctgttcca tgaccaggga cagattgcca ccaagcttca ttcctttgat
     4261 gtgggcataa ctgtagccgc agggcttcca tttgctatta caacaccgga acatggcact
     4321 gcatttgata ttgcaggcaa gggaatagca aaaactctgg ccacagagcg ggctattgca
     4381 gttgcagcta gaatggcatc
//
LOCUS       DACADF010000246         4348 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.248, whole genome shotgun sequence.
ACCESSION   DACADF010000246 DACADF010000000
VERSION     DACADF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.248"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            93..1001
                     /locus_tag="ISB30_004637"
     CDS             93..1001
                     /locus_tag="ISB30_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP1992190.1"
                     /translation="MKAPVSITSFRPQKSIAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1208..3199)
                     /gene="tkt"
                     /locus_tag="ISB30_004638"
     CDS             complement(1208..3199)
                     /gene="tkt"
                     /locus_tag="ISB30_004638"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP1992191.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3477..4235
                     /locus_tag="ISB30_004639"
     CDS             3477..4235
                     /locus_tag="ISB30_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAP1992192.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQDMDSKATIAPANSEYAKRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
BASE COUNT         1046 a         1146 c         1156 g         1000 t
ORIGIN      
        1 taccccttta tagattctga attctcacct gccagctgtt ttctcctccc cccatgtgac
       61 atattcccat actttactca aggaagattc atatgaaagc acctgtttca ataacctcat
      121 ttcgcccaca aaaatctatt gctatcgcca ttggcgtgct tgccgtggtt gtgctgccat
      181 ttctctcgta ttacaccgtt aatgaaggtg aacgcggcat tttgctgcgc tacggaaaaa
      241 tcgtaaaagt tgccgagccg gggctgggtt ttaagatccc ttttatggaa agtgtagaga
      301 aaatttccac gcgtaatcag gctgtggtct atcagggatt acaggcctac agccgcgacc
      361 agcaaccggc acaaatgacg gtatcagtca gcttccacat taaaccttca gaagcaggcg
      421 cggtttatac cacctacaac accattgaag cattaaaaga tcgtctgatt gtccgtcagt
      481 tgccaactca actggagaac gttttcggcc aatataccgc catcagcgcg gtacaggatc
      541 gtacaaaact ggttcaggat ctgcaaaatg caatgcgtaa agccgttgtt ggcccggtgg
      601 tgattgatgg tgtccagatt gaaaatattg atttttctga cgcttatgaa aagtcgattg
      661 aagaccgaat gaaagcagaa gtcgccattg ccacacgtaa gcaaaatctg gaaacagaga
      721 aaatccaagc gcaaatagca gtaactcagg cccaggcaga agcagacagt aaactggcgg
      781 cagccaaagc agaagctgag accattcgcg taagaggcgc ggcagaggct gaaactattc
      841 gtttaaaaag tgcggctgaa gcagaagcta tccgcctgcg cggtgaagct ctgcgggaga
      901 atcccggact tgttgccctc accactgctg agcgttggga cggtaaactg cccgatacca
      961 tgatccccgg cagcacagtt ccgtttatct ctactaagta aaaaaaggcc gctttcgcgg
     1021 cccttgagac tgatagcaaa gacaaaactg cctgatgcgc tacgcttatc aggctgcaat
     1081 atgttgaatt tgcaaaatct tgtaggacgg ataaggcgtt cacgccgcat ccggcataaa
     1141 caacgcgcac tttgccaaca atctgaaggg ccgcatttgc ggcccttctc acaaagcatc
     1201 ttaccgatta cagcagcgct tttgctttcg caacaacgtt gtcaacggtg aagccgaact
     1261 cttcaaacag ctgctctgcc ggagcagatt caccgaaggt ggtcatgccg acgatagcac
     1321 cgttcaggcc aacatacttg taccagtagt cagcaatgcc agcttccacc gcaacacgag
     1381 cggtaaccgc tttcggcagt acggattcac ggtaagccgc gtcctgcttg tcgaatgcgt
     1441 cggtagacgg catggaaacc acgcgcgctt tcacgccttc ggcagtcaat ttttcgtatg
     1501 ccgctacagc cagttcaact tcagaaccgg tcgcgatgaa gatcagttcc ggctgaccag
     1561 cacagtcttt cagcacataa ccaccgcgcg cgatgtttgc cagctgctct tcagtacgtt
     1621 cctgctgcgc caggttctga cgggagagga taagcgcagt cgggccgtcc tgacgttcaa
     1681 cgccgtattt ccacgctacc gcggattcaa cctggtcaca cggacgccat gtagacatgt
     1741 tcggggtcac gcgcagagaa gcgacctgct ctaccggctg gtgagtcggg ccatcttcgc
     1801 ccagaccgat ggagtcgtgg gtgtaaacca tcacctgacg ctgtttcatc agcgcagcca
     1861 tacgtacggc gttacgtgcg tattccacga acatcaggaa ggtggaggtg tacggcagga
     1921 aaccaccgtg cagggagata ccgttggcaa tcgcagtcat accgaattca cgtacaccgt
     1981 agtggatgta gttacccgca gcatcttcgt tgattgcttt agaaccagac cacagggtca
     2041 ggttagacgg cgccaggtct gcagaaccgc cgaggaattc cggcaacagc ggcccgaacg
     2101 cttcgatagc attctgagac gctttacggc tggcgatctt cgccgggtta gcctgcagtt
     2161 tagcaatgaa ctctttcgct ttcgcgtcga agtcagacgg catttcgcct ttcatacggc
     2221 gggtaaattc agcggcttcc tgcggataag ctttcgcgta agcagcgaat ttctcgttcc
     2281 atgcggactc tttcgcctgg cctgcttctt tcgcatccca ctgagcatag atttcagacg
     2341 ggatttcgaa cggcgcgtat ttccagccca gctgttcgcg ggtcagggca atttcagcgt
     2401 cgcccagcgg cgcaccgtgg gagtcgtggg taccggcttt gttcggggaa ccgaaaccga
     2461 tgatggtttt gcacatcagc agggacggtt tgtcagtcac tgcgcgcgct tcttctactg
     2521 cgcgtttgat ggatgccgcg tcatgaccgt cgatgtcgcg aataacgtgc cagccgtaag
     2581 cttcgaaacg cattgcggtg tcgtcggtaa accagccttc aacgtgacca tcgatagaaa
     2641 taccgttgtc atcgtagaac gcaatcagtt tacccagctt cagcgtaccc gccagagagc
     2701 aaacttcgtg ggagatgcct tccatcatgc agccgtcgcc catgaaggcg taggtgtagt
     2761 ggtcgacgat gtcgtggccc ggacggttaa actgcgccgc cagcgttttt tctgcaatcg
     2821 ccataccgac tgcgttggca ataccctgac ccagcggacc ggtggtggtt tctacacctg
     2881 cggtgtaacc cacttccggg tggcccggag ttttagagtg cagctgacgg aagtttttca
     2941 gttcttccat cggcagatcg taaccggtga ggtgcagcag gctgtagatc agcatggagc
     3001 cgtggccgtt ggacagcacg aagcggtcac ggtcagccca ggacggattc tgcgggttgt
     3061 gtttcaggaa atcacgccac aggacttcgg caatgtcagc catacccata ggggcacccg
     3121 ggtgaccgga tttggctttc tgtactgcgt ccatgctcag cgcacgaata gcattggcaa
     3181 gctctttacg tgaggacatt ttgactccag atcggatgat gaagggcacg cccttaacga
     3241 cttgacgaca gcgcgttttg ggctacgccg gaaaatttgc caacaattta ccgcaagccg
     3301 cgcgtcatgt acatggaaca tccttttgcc gcttcagaaa tctctggatc atgctcgcat
     3361 gttgcgcaat ctactcgccg gtccactgca cttttcctta tacttcgatt gagcgtcaat
     3421 tcacctgcag atggcgcatt tttagaataa tcctgacctt gtgcggaaga gaaaacatga
     3481 aaattcgcgc cttattggta gcaatgagcg tggcaacggt actgactggt tgccagaata
     3541 tggactccag tggactgctc tcatcaggag cggaagcttt tcaggcttac agtttgagtg
     3601 atgcgcaggt gaaaaccctg agcgatcagg catgtcagga tatggacagc aaggcgacga
     3661 ttgcgccagc caatagcgaa tacgctaaac gtctgacgac aatttctcgc gcgttaggcg
     3721 ataacatcaa tggtcagccg gtgaattaca aagtgtatat ggcgaaggat gtgaacgcct
     3781 ttgcaatggc taacggctgt atccgcgtct atagcgggct gatggatatg atgacggata
     3841 acgaagtcga agcggtgctc ggtcacgaaa tggggcacgt ggcattaggc catgtgaaga
     3901 aaggaatgca ggtagcactt ggtacaaacg caattcgcgt tgcggcggcc tctgcaggcg
     3961 gaattgtcgg cagtttgtca caatcacaac taggagacct gggcgaaaag ctagtcaatt
     4021 cgcaattctc ccagcgccag gaagcagagg ccgatgatta ctcctacgat ttgctgcgcc
     4081 aacgcggcat cagtccggct ggccttgcca ccagctttga aaaactggct aaactggaag
     4141 aaggtcgcca aagttcaatg tttgacgacc acccagcatc cgccgaacgc gcccaacata
     4201 ttcgcgatcg tatgagcgcg gatggagtta agtaaggcat ggtggtgcca aacgtaggtc
     4261 agataaggcg tttacgccgc atctgacatg aatggcacat ttgttatcct gtgcgcgttg
     4321 ccggatgcga tgctggcgca tcttatcc
//
LOCUS       DACADF010000247         4327 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.249, whole genome shotgun sequence.
ACCESSION   DACADF010000247 DACADF010000000
VERSION     DACADF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.249"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..505
                     /locus_tag="ISB30_004640"
     CDS             <1..505
                     /locus_tag="ISB30_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992193.1"
                     /translation="FVVSPQWHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSML
                     VSITWNFIVGFCVLGTALAIRVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGL
                     GLLMLGVASTAGQLLGSVLIDVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRTSK
                     TMKKMEV"
     gene            508..1542
                     /locus_tag="ISB30_004641"
     CDS             508..1542
                     /locus_tag="ISB30_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HAP1992194.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(1582..1910)
                     /locus_tag="ISB30_004642"
                     /pseudo
     CDS             complement(1582..1910)
                     /locus_tag="ISB30_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420459.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltranspeptidase"
     gene            complement(1954..3573)
                     /locus_tag="ISB30_004643"
                     /pseudo
     CDS             complement(1954..3573)
                     /locus_tag="ISB30_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3857..4267)
                     /locus_tag="ISB30_004644"
     CDS             complement(3857..4267)
                     /locus_tag="ISB30_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992195.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPSCGTGVYSREGGLQEGNWKDRN"
BASE COUNT         1040 a         1137 c         1021 g         1129 t
ORIGIN      
        1 ttttgttgtg tcaccgcagt ggcattctac ctctttcatc ttacttgcca ttcttccgtt
       61 cttagctggt ttgcttgcag gatggcagcc cgctggaaat gcaaaagtag ctgaagctac
      121 gggatccatg ctggtttcaa tcacctggaa tttcatcgtt ggattttgtg ttttaggcac
      181 agcgctagct attcgtgtcg cattagggca cgtcactgtt caattaccag atgtctggtg
      241 gatgtatctt ggcggcccgc ttggactaat gtctatcggt ttaatggcca ttttggtcag
      301 aggtctgggg ttactgatgc tgggggttgc ttcaacggcg ggccagttac tgggatcagt
      361 attgattgac gtattaattc catcgctggg caataccgtt tatctggtca cgatcatcgg
      421 aacactgttt gcactggtcg gtgcaattgt taccactatt ccggaataca gaacctcgaa
      481 aacaatgaag aaaatggagg tataatcatg aaagattatc tacaaactgt gacgggcccc
      541 gtcgcccgtg aagatatggg gctaacactt cctcatgaac atctgttcaa cgatctttcc
      601 tctgttgtgg atgctccttg ctatcctttt tcacaacggc ttgttgataa aaaagtcacg
      661 gcagaaatcc agtgggcatt aaaacacgat ccttattgct gcgctgataa tatggaccgc
      721 aaacctattg aagatgtgat cttcgaaatt aataacttta tctcgctcgg tggtcgcacc
      781 attgttgatg ccacagggtc tgagtctatc ggacgagatg cgcaggcatt acgagaagtc
      841 gcattaaaaa caggtctgaa tattgttgct tcctccggtc cctacctgga aaaatttgaa
      901 agccagagaa ttcataaaac ggttgatgaa ctggcggcaa cgatcgataa agaattgaat
      961 caagggattg gcgatacgga tattcgtgcc ggaatgatcg gtgaaatagg tgtctcaccg
     1021 acatttaccg aagccgagca taacagcttg cgggctgcct cgctggcaca gattaacaat
     1081 cctcatgtgg cgatgaatat tcacatgccg ggctggcttc gtcgcggtga tgaagtactc
     1141 gacattgtgt taggtgaaat gggcgtctcg ccaaataaag tctctctcgc acactcggat
     1201 ccgtcaggaa aagacgtggc gtatcagcgg aaaatgcttg ataagggtgt ctggctggaa
     1261 ttcgacatga ttggcctaga cattaccttc ccgaaagagg gaatagcgcc aggggtgcag
     1321 gagactgccg atgccgtcgc tcatctcatt gagttgggat acgccgatca gcttgttctc
     1381 agccacgatg tcttccttaa acaaatgtgg gctaaaaatg gcggtaatgg ctggggattt
     1441 gttccagatg tttttctggc ctatctggcg gagcgcggcg tcgataaaac gatcctcaaa
     1501 aaactctgta tcgataatcc cggacgatta ttgaccgcat aaaaccatta tcccccggca
     1561 cactgcgccg ggggatgtca attaataccc cgccgttaaa tcatccacca agcgcgagtc
     1621 ggatgcgccg tacaactcac catccgaccc aaccataatg ctttgcgtgc tgcccatcgc
     1681 ctcattcagc gccactttct gaccttttgc ttccagcagc ttgagcgtat ccggactaaa
     1741 cccttttcaa cacgcagcta gtccggcaac cactgatggt ggaaacgagg cgcattggtc
     1801 gcttcggcaa cattcattcc acaattgatg ctgttcacca ccatttgcag cacggtagtg
     1861 atgattcagc taccggtaac aaagtgagta cataaaggcc atactgtcac gaacagtgca
     1921 aaagtggctc ttccgcaacc taggtgaaga ttttcatgcc agtggactca ttaccctccg
     1981 cctaagtagt tcaaatccgg ctcgaccata catctggcgt atcagcacct tcaggcgatt
     2041 tacatgacct tcgacaacac cattactcca gcgactgctg attgcctcac atatagccgc
     2101 agcatcagct tccatccccg cagctacgca ccgaagttct gctgagccgc tttcgtgaac
     2161 tcgagtgaac cagctgctta gctgtgattt attttaggtt ttcagaatac ggtagaactc
     2221 gagtaccagt tgctgtgcta ttttcaactc cggttctttt tcacacatca gactaataaa
     2281 tcgggatgca taattttctt cgcccctgat tattctccag ggcatcaacc agcggcttac
     2341 ccgggacact gaaggaagtc ttgcggcagt agtaaccggt gggttccagc ctttacgcca
     2401 tttggcaaca gtatccctga cgatggtttc actgcctgtc cccccctggg ccaccatttc
     2461 ccgccatatc cggctggcat tataattacc actttctcgt tgctctttta accactccct
     2521 ccatgggtcc agaagccctc gctttggagg ccgggttgac atttcaggga atgttcctga
     2581 acgcacccag cgactgactg tcactcgcga caggcctgta atacgggata tttccctgaa
     2641 actacatccc ttgttatgca gggccataac ctctgtccaa tgctgatgcc gttttttgcg
     2701 ggtttgctgt ttaaggcgtt ccggacgacg gagcgatgct acaggcacag atatttctgg
     2761 ctcaggtgat ttcttcagtg ataactctct gacaacaaga cgtatcagag gcatatgtct
     2821 gtacatcatt cgttcaggct catcaccaat actttttagc aggtgccatc gatcggccac
     2881 ctgtctggcc tgaggtgcac cttcacgtgc tgctgtcgca tagactccac tgcgatcacg
     2941 cgagacaacc tgtatttccg gatattttct gaaccaggtc gccagcgtac gctgatcacg
     3001 gccgggaagc aggacgaggg gacggtgagt atcaagattg actattaaca taccgcagca
     3061 gtggccccga tgccacgccc actcatcaat accgacatga ggcgcgcctg actgtttcgt
     3121 ctccggggta ttgataaccc tgcgaagaag agtatcagca ctgcaacgga gacccgctgc
     3181 cgttgcagcc cgttttccag cctcacctcc agctattaat cccagttgac gttgtaaatt
     3241 ttgtaacgcc tgtgaagact gctggtgtga accggcgaag ggagcaagcg actcggcaaa
     3301 aattttacgt taacaagccg ggttacggca gtaccagtgg cggacggaaa atacaagcca
     3361 tagcgtctgc cctgagcagg gtagatgctg tatccggcgc cgacgacaac tatgaacaga
     3421 gtcgctacgc ttaaagcatt caggacattg tgctgttttg cgttttccgt ggagatggag
     3481 ggttataccg tcagagctaa taatttgtcg actgcatcgc catccgcaag gtaactgtag
     3541 aagtgtctta agagagtgca ttgcgttgcc cataacttaa aacagcttag atccttgttc
     3601 gccagaaagt ttcatccatt ttcttccacc caggttgcgg aagaaccact tttcatccgt
     3661 cgttgacaga tgtgaagtat gcgggagaaa aagtgaatat ctaactgtaa taactcagac
     3721 cggtacgagt acccgggacg tctacaatca ttcagggcaa agtgctaaaa tttctctatc
     3781 ccgggatgcc cctaccaacg cagcactccc ggagatatca ttacagctct acaactgatt
     3841 tccgcgactg aaccgctcag ttcctatcct tccagttgcc ttcctgcagc cccccctccc
     3901 tgctgtatac cccggtacca caactcgggc agcacttttc tccttcataa tcttcgtcac
     3961 acatcacaca aaaccacttt ctcaccttca ccgtataatc cggaaccgtc ataccctgca
     4021 gatgcaaacg acgggcctct ttctctgccg ggtcaagcca ggtgcatcgc agcaggattt
     4081 tctcagcgac agtcatctgg tcatgttcaa accacggtgg ttgtccgtca ttgccccgct
     4141 gaagtgttaa cgggcatcca caggacacac agcacaaaac gtcctggcgg ttgtgcttcg
     4201 ctgcttcttt cgccgtaaca aaatggcctt tgctgtcatt cgccatataa cagttcagaa
     4261 ttcgcatatt tcctcctgag ttgtccgggg aatactgaat cctccggtct ggcagtgtgt
     4321 gcagtac
//
LOCUS       DACADF010000248         4310 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.250, whole genome shotgun sequence.
ACCESSION   DACADF010000248 DACADF010000000
VERSION     DACADF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.250"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            9..548
                     /locus_tag="ISB30_004645"
     CDS             9..548
                     /locus_tag="ISB30_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HAP1992196.1"
                     /translation="MFATALLSGVAFNALADDPNQGSGKITFKGEVIDAPCSIAPGDE
                     DQTINLGEVADTVLKSGQKSLPVDVTIHLQDCILSDGTNTVDKVKITFSSASVDATDS
                     NLLKNTLEGNIGGATDVGVRLVKSDNTNVTLGTPITINFPTTNSYQELNFKARMESLG
                     RTATPGNVQAQANYVLDYK"
     gene            633..3155
                     /locus_tag="ISB30_004646"
     CDS             633..3155
                     /locus_tag="ISB30_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP1992197.1"
                     /translation="MNNKNTFTGDTLSHAIKNALSGVVCSLLFVLPAHAVEFNVDMID
                     VEDRENIDISRFEKKGYIPPGRYLARVQINKNMLPQALILEWVKADNESGSLLCLTKE
                     DLTSFGLNTEFIESLQTIAGRECLDLSQRQELTTRLDKATMILSLSVPQAWLKYQATN
                     WTPPEFWDTGIAGFILDYNVYASQYAPHHGDSTQNVSSYGTLGFNLGAWRLRSDYQYN
                     QNFADGRSVNRDSEFARTYLFRPIPSWSSKFTMGQYDLSSNLYDTFHFTGASLESDES
                     MLPPDLQGYAPQITGIAQTNAKVTVAQNGRVLYQTTVAPGPFTISDLGQSFQGQLDVT
                     VEEEDGRTSTFQVGSASIPYLTRKGQVRYKTSLGKPTSVGHNDINNPFFWTAEASWGW
                     LNNVSLYGGGMFTADDYQAITTGIGFNLNQFGSLSFDVTGADASLQKQNSDNLRGYSY
                     RFNYAKHFESTGSQITFAGYRFSDKDYVSMSEYLSSRNGDESIDNEKESYVISLNQYF
                     ETLELNSYLNVTRNTYWDSASNTNYSVSVSKNFDIGDFKGISASLAVSRIRWDDDEEN
                     QYYFSFSLPLQQNRNISYSMQRTGSSNTSQMISWYDSSDRNNIWNISASATDDNIRDG
                     EPTLRGSYQHYSPWGRLNINGSVQPNQYNSVTAGWYGSLTATRHGVALHDYSYADNAR
                     MMVDTDGISGVEINSNRTVTNGLGIAVVPSLSNYTTSMLRVNNNDLPEGVDVENSVIR
                     TTLTQGAIGYAKLNATTGYQIVGVIRQENGRFPPLGVNVTDKATGKDVGLVAEDGFVY
                     LSGIQENSILHLTWGDNTCEVTPPNQSNISESAIILPCKTVK"
     gene            3171..3911
                     /locus_tag="ISB30_004647"
     CDS             3171..3911
                     /locus_tag="ISB30_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP1992198.1"
                     /translation="MKSPLITTGMICLLGICNFAQATVSPDRTRIIFNASNKSATVRL
                     TNQSKIDPYLAQSWIEDASGKKTRGYISTLPPMERIEPDEQVQIRLMALASLNNLPQD
                     RETLFYYNVREIPPRTKEQNVMQIAMQSRLKLFWRPKAIELKEGQMVPLQKVTITRTA
                     AGLTLNNPTPYHITVGYIGTNGKTLMPGADSIMVTPFASATQYLNNLPSTFQLGFVAD
                     YGGLEMFKVECNSVQSLCQSSPAKKGKI"
     gene            3908..>4310
                     /locus_tag="ISB30_004648"
     CDS             3908..>4310
                     /locus_tag="ISB30_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAP1992199.1"
                     /translation="MITLFRLLAILCLFYNVSVYAVNCYQDQRGGNTQIRGTLPSFRI
                     PENAQPGQKIWESDDVNVTVYCDNATGWRADDPAENIYAWIKMPAINSADILNNPYLT
                     FGVTYNGVDYEDSDVGIDTFACLDKYEQYYGG"
BASE COUNT         1223 a         1007 c          963 g         1117 t
ORIGIN      
        1 tgttatctat gttcgcaacc gcattgttat ctggcgttgc ttttaacgcg cttgctgatg
       61 acccaaatca gggttctggt aaaataacat ttaaaggtga agttatcgat gcgccttgtt
      121 ctattgctcc tggtgatgaa gatcagacaa taaacctcgg tgaagttgct gatactgtat
      181 taaaaagcgg acagaaatct cttcctgtag atgtcaccat tcacttgcag gattgtattt
      241 tatctgacgg cactaacact gttgataaag tcaaaatcac ctttagttct gccagtgttg
      301 acgctaccga ctccaacctg cttaaaaaca ctctggaagg taacatcggc ggcgcaactg
      361 atgtaggcgt acgtctggtg aaatcagaca acaccaacgt gactcttggc actccaatca
      421 ctatcaactt cccgacgact aactcctacc aggagttgaa ctttaaagcc cgtatggaat
      481 ctctgggacg caccgcgacc ccgggtaacg tgcaggcaca ggccaattac gtactcgact
      541 acaagtaagc ggcaattaat acgcacaacg tcagacggta aagccgatga tatgtcatcg
      601 gcaactttgg gtcttttttg aaatggattt ttatgaacaa taaaaacacg tttaccgggg
      661 atacattatc ccacgcaatt aaaaatgccc tgtctggcgt agtgtgttcg ctactcttcg
      721 ttttacctgc ccatgcagtg gaattcaacg ttgatatgat tgatgttgaa gaccgtgaga
      781 atatcgacat ctctcgtttt gagaaaaaag gctatatccc cccaggcaga tacctcgctc
      841 gtgtgcaaat aaataaaaat atgttgccac aagcgttaat actggaatgg gtaaaagccg
      901 ataatgaaag tggttcgtta ctctgcttaa ccaaagaaga tctgactagt ttcggtctta
      961 atacggaatt tattgaatca ttgcaaacca tagctggcag agaatgtctc gatttaagcc
     1021 aacgtcagga gttaacgaca cggcttgata aagctacgat gatattatcg ctaagtgttc
     1081 cccaggcgtg gttaaaatac caggcaacaa actggacgcc accagagttt tgggataccg
     1141 gtatcgccgg gtttatcctt gattacaatg tgtacgccag ccaatatgcc ccacatcatg
     1201 gagacagcac ccaaaacgtc agctcctatg gtacgttagg ctttaacctc ggcgcatggc
     1261 gcttacgtag cgattaccaa tataatcaga attttgctga tggacgctcg gtaaaccgcg
     1321 acagcgaatt tgcgcgaact tatctgtttc gccctatccc ctcctggtcg tcaaaattca
     1381 ctatgggcca gtacgacctg agctccaatc tttacgatac cttccacttt actggcgcat
     1441 cgctggaaag tgatgaaagc atgctgccgc cagatttaca gggttatgcg ccacaaatta
     1501 ccggcatcgc gcagaccaac gcgaaagtaa ctgtggcaca aaatggtcgt gtactttatc
     1561 aaaccactgt cgcgccaggc ccttttacta tttctgattt ggggcaatcg tttcaggggc
     1621 agctggatgt cacagtggaa gaagaagatg gccgcaccag caccttccag gttggatccg
     1681 catccattcc ctatttaacc cgtaaagggc aagtgcgcta taaaacgtcg ctgggaaaac
     1741 cgacatccgt cgggcataac gatatcaata atcccttttt ctggacggcg gaagcctcct
     1801 ggggctggtt gaacaatgtg tcgctgtatg gtggcggcat gttcaccgct gatgattatc
     1861 aggctatcac taccggtatt ggctttaatc ttaaccaatt cggttcgctt tcttttgatg
     1921 tcaccggagc agacgcgtct ttgcagaaac aaaatagcga caatctgcgt ggttacagct
     1981 atcgcttcaa ctatgcaaag catttcgaat cgacaggtag tcagattacc ttcgcgggtt
     2041 atcgcttctc agataaagat tacgtgtcga tgagtgagta cctaagctcg cgtaatggcg
     2101 atgagtcaat tgataatgaa aaagagagtt atgtcatttc cttgaaccag tactttgaaa
     2161 cactggaatt aaactcttat ctcaacgtta cacgcaatac ttattgggac agcgccagca
     2221 ataccaacta ctccgtatct gtaagcaaaa actttgatat tggcgatttc aaaggtatct
     2281 ctgcatcgct ggcagtaagt cgaatccgct gggatgacga cgaagagaat caatattact
     2341 tctctttctc tctaccttta caacaaaacc gcaacatctc ctacagtatg cagcgtacgg
     2401 gaagcagtaa tacttcgcag atgatttcct ggtacgattc atcagatcgc aacaatatct
     2461 ggaatatttc agcgtcggca acggacgaca atatacgtga tggcgaacca acactgcgcg
     2521 gcagctacca gcactattca ccgtggggac gtctgaacat taacggcagt gtacagccga
     2581 atcagtacaa ttctgttacc gcaggctggt acggttcact taccgctaca cgtcatggtg
     2641 tcgcccttca cgattatagc tatgctgata acgcccgcat gatggtcgat accgacggca
     2701 tctccggcgt tgaaatcaac tctaaccgaa ctgttaccaa cgggctgggc attgccgttg
     2761 taccttcgtt atcgaactac accacctcca tgttgcgggt gaacaataac gatctgccag
     2821 aaggtgtcga tgtcgaaaac tcggttattc gtactacgct cacccagggt gccatcggct
     2881 acgcaaaact gaatgccacc accggatacc aaatcgtcgg cgttattcgt caggaaaatg
     2941 gccgcttccc tccactaggt gtgaatgtca cggataaagc gacaggtaaa gatgtgggcc
     3001 tggtcgcgga agacggcttc gtttatctca gcggtattca ggaaaacagt attctgcatt
     3061 taacctgggg tgataatacc tgtgaagtca cgccgccaaa ccaaagtaac attagcgaaa
     3121 gcgcaataat tttaccttgt aaaacagtca aataattcag gatgaatata atgaaatcac
     3181 cattaatcac aacaggaatg atttgtttac tggggatctg caattttgcc caggcgactg
     3241 tctcccccga caggacacgt atcattttta atgcttcgaa taaaagcgca acggtccgtc
     3301 tgactaacca aagtaaaatc gatccctatc tggctcagtc gtggattgaa gatgccagcg
     3361 gtaaaaaaac gcgtggttac atatcaacac tgccgccgat ggaacgtatt gagccggatg
     3421 aacaggtaca aattcgcctg atggcgctgg cctcattaaa caacttaccg caggacagag
     3481 aaacgctgtt ctactataac gtgcgggaaa ttccgcctcg cactaaagaa cagaatgtga
     3541 tgcaaatcgc catgcagagt cgactgaaat tattctggcg tccgaaagca atcgaattaa
     3601 aagaagggca gatggtgcct ttacaaaaag tcacaataac acggaccgct gcagggctga
     3661 cactcaataa tccaacgcct tatcacatca ccgtcgggta tatcggtact aacggcaaga
     3721 cactgatgcc cggtgcagac agcattatgg tgactccgtt tgcttctgca actcaatact
     3781 tgaacaactt gccctcaact ttccagttag gctttgttgc tgactatggt ggactggaga
     3841 tgtttaaagt cgaatgtaat tccgtccaat ccctctgcca aagctctcct gctaagaagg
     3901 gaaaaatatg attacgcttt tccgtttact ggcgattctt tgcctttttt ataacgtttc
     3961 agtttatgct gtaaattgct atcaggatca aagaggtggt aatacacaaa tacgtggtac
     4021 tttaccttcc ttcagaattc cagaaaacgc ccagccggga caaaaaattt gggaaagcga
     4081 tgacgttaat gttacggtat attgtgataa cgcgacagga tggagagctg atgatcccgc
     4141 agaaaatatt tatgcctgga ttaaaatgcc ggctataaat agtgcagaca ttttgaataa
     4201 cccatacctg acattcggtg taacctataa tggtgtggac tatgaagata gcgatgtagg
     4261 aattgatact ttcgcctgtc tggataagta tgaacagtat tatggtggta
//
LOCUS       DACADF010000249         4282 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.251, whole genome shotgun sequence.
ACCESSION   DACADF010000249 DACADF010000000
VERSION     DACADF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.251"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(13..1410)
                     /gene="ccp"
                     /locus_tag="ISB30_004649"
     CDS             complement(13..1410)
                     /gene="ccp"
                     /locus_tag="ISB30_004649"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAP1992200.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYMPYMQDKQ"
     gene            1815..3464
                     /gene="treF"
                     /locus_tag="ISB30_004650"
     CDS             1815..3464
                     /gene="treF"
                     /locus_tag="ISB30_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAP1992201.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3515..4117)
                     /locus_tag="ISB30_004651"
     CDS             complement(3515..4117)
                     /locus_tag="ISB30_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP1992202.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         1092 a         1113 c          993 g         1084 t
ORIGIN      
        1 cgatcaaatt aattattgtt tatcctgcat atacggcatg taaaccccgt tcagactgtg
       61 caggaaagct acgatatcat ccacatcctc ctgcggcagc tctttgccta cctgatagcg
      121 cagcatcagt ttcactgccc cgtccagcgt cggcacgtca ccgcgatgga agtacggtgc
      181 cgttaaagca acgttacgta aaccgggtac tttctgacgc aatttatcac gctcttcttt
      241 agtaacattc atacgaccaa tatccgccgc cgtaatttca ccaaagttaa agtctttttt
      301 cagccccaac ggttcaaagg aacgtccgcc gagaataata ccaccatgac aagttgcaca
      361 tttattatct ttaaataatt gatagccttt tttctgttgc gccgtcagag cattctcatc
      421 tccacgcaac catttatcaa atggggaatc cggcgtaatt aatgttttct caaattcagc
      481 aatggcatca gtaatatttt cgccactgaa accttgcgga tagacttcga ggaactgcgc
      541 tttaagctgc ggatcttttt ccagcttagc aataatttcg tcccaggatt tcgacgccat
      601 ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc aatgttgccg cacgaccatc
      661 ccagaactgc tcaacgttaa atactgagtt aaataccgtc ggcgcgttaa tcggcccaac
      721 tgcgccacca acaccaatcg atgtttttct gccatcgacg ccccccgcat tcaacgcatg
      781 gcaatgagcg catgaaatgg tgctatccgc cgataaacgg ggatcgtgat acagcgcaaa
      841 acccaacgcc actttttgcg catcggtagg cagtttttgc gggatgggct gcaccggttc
      901 attgcgatgc tccggagcag tatcattgct ggcgtaatat tccgcgcgct gttttgcaat
      961 ccaggccagt atttccgccc gctcttcatc actcacctta cccgcccagt gtagcgcggt
     1021 ataacgcgtt ggcggcatag tttcatactg catcacccat tcaatcttat tcagatcgct
     1081 ttgcgaaacg ggtttattag ccagcagagc cgcacgcacg gcctcgaggt taaaagattt
     1141 atatccaagc tttatgtcgt aatccatcaa ctgtttcgcg ccgggaatat aataataggc
     1201 gggtaattct gccgaaggcg tatggcaata atcgcatcct ttttcgcgga gaaagcctaa
     1261 aaccttatta ttttcactga cagcagatgc ctgaacatcg gcctgtttac tgcgtttatt
     1321 atcgtggtac cacacataac cagataaccc taaatagcaa atcgcgacgc cagccaggcc
     1381 gatcgcggta atacgtgaga ccattttcat tattcttccc tcacggtagt gagttattgt
     1441 gacccagaaa attttccaga atgctgcaaa cgtctctgcg agacaaagcc atcataaaaa
     1501 acagggagat atgctttttg ataggaatca atcaattaca tgaatataaa ctatcaataa
     1561 gatagccagt gcctattacc cagatcagga atggcatatc atgattgcgg cgagagcagg
     1621 attgaatgtt ctgaaaatga aaaacaacac gaaatccgta tttttataaa ttccattgat
     1681 attagtgcgt tatatttttt ggcaggttaa taaacgtgat ccaccgcacg ctttgtcgcc
     1741 caccaggcgg agcgaatgac tacccttaaa gaaaagcccg ataattagcg acgaatttcg
     1801 gaggtcggat ccttatgctc aatcagaaaa ttcaaaaccc taatccagac gaactgatga
     1861 tcgaggtcga tctctgctat gagctggacc cgtatgaatt aaaactggat gagatgatcg
     1921 aggcagaacc ggaacccgag atgattgaag ggctgcccgc ctctgatgcg ctgacgcctg
     1981 ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc gaaaattttc cccgacagta
     2041 aaacctttcc cgactgcgca cccaaaatgg acccgctgga tattttaatc cgctaccgta
     2101 aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt tgaaaaccac ttctggctgc
     2161 cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa ttccctgaaa gagcatatcg
     2221 accagctgtg gccggtgcta acccgtgaac cacaggatca cattccgtgg tcttctctgc
     2281 tggcgctgcc gcagtcatat attgtcccgg gcggccgttt tagcgaaacc tactattggg
     2341 attcctattt caccatgctg gggctggcgg aaagtggtcg ggaagatttg ctgaaatgca
     2401 tggccgataa cttcgcctgg atgatcgaaa actacggcca catccccaac ggcaaccgca
     2461 cctattattt gagccgctcg caaccaccgg tttttgcgct gatggtggag ttgtttgaag
     2521 aagatggtgt acgcggcgcg cgccgctatc tcgaccacct gaaaatggaa tatgccttct
     2581 ggatggacgg tgcagaatcg ttgatcccta atcaggccta tcgccatgtt gtgcggatgc
     2641 cggacggctc gctgctcaac cgttactggg acgatcgcga cacgccgcgt gacgaatcct
     2701 ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc gcctaacgag gtgtaccgcg
     2761 atttacgtgc gggggcggcc tccggttggg attactcttc ccgttggctg cgtgatactg
     2821 gtcgtctggc gagcattcgt accacccagt tcatccccat cgatctgaat gccttcctgt
     2881 ttaaactgga gagcgccatc gccaacatct cggcactgaa aggcgagaaa gagacagaag
     2941 cactgttccg ccagaaggcc agtgcccgtc gcgatgcggt aaaccgttac ctctgggatg
     3001 atgaaaacgg tatctaccgc gattacgact ggcgacgcga gcaactggcg ctgttttccg
     3061 ctgccgccat tgtgccgctc tatgtcggta tggcgaacca tgaacaggcc gatcgtctgg
     3121 caaacgccgt gcgcagccgg ttactgacac ctggcgggat tctggcaagc gagtacgaaa
     3181 ccggtgaaca gtgggataaa cccaacggct gggcaccgtt acagtggatg gcaattcagg
     3241 gatttaagat gtacggcgat gaccttctgg gtgatgaaat cgcgcgcaac tggctgaaga
     3301 cggtgaatca gttctatctg gaacagcaca aactgatcga gaaataccat attgccgatg
     3361 gtgttcctcg cgaaggcggc ggtggcgagt atccgttgca ggatgggttt ggctggacta
     3421 acggtgtggt acgccgttta attggtttgt acggcgaacc ataatatttt tacagccagc
     3481 cgctaacttc ctgctggctg taaaattatc ctcttcagga ggagatattt aacatcattg
     3541 ccgcctgggt gcgatttttg acttccagac ggcgatacag tgattccaga tgcgctttta
     3601 ccgttccggt actgatattc agcgccctgc cgatctcttt atttgattcg cccgccgcta
     3661 acatggttaa aatctcccgc tggcgggcgc ttaacgattt gagatcttta atgtcctttt
     3721 ccggcgtcgt tcgccagtct ccaggcagaa acatcatccc catcgccgca ctatttaccg
     3781 ccaacgcaaa tgtctcgacg gttgaatcac gaggcacaat ggccagcaca ttaaaatgaa
     3841 taacttcctg taaccaccgt ttgttgcaat ccgtcgccgt aattaacacc ttaacctcag
     3901 gaaattgcac cacagttttt tgcagcaacc agtagcaaaa ctcaccatcc tgatcgccat
     3961 cgagcatcac taaggcttca gggtaacttt ccagcttttg ccataactcg tctgcctgac
     4021 tggccccctg aatactcact cctggaatac gatgctgtaa actgattttc attccatgaa
     4081 taaatattga ctgcctgtca aacatgacta tttgcataac tgaatctcca cctgaatacg
     4141 ttaaaaagac ttaagtagtg gaagggtatt acccgcgaga aaaaataaga attcgccatt
     4201 tggcggtggc cattctacag agatgacacg tagaaaatag ttaccgatat aaatagttac
     4261 agctaaacgc ctgaaattac at
//
LOCUS       DACADF010000250         4278 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.252, whole genome shotgun sequence.
ACCESSION   DACADF010000250 DACADF010000000
VERSION     DACADF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.252"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..299)
                     /locus_tag="ISB30_004652"
     CDS             complement(<1..299)
                     /locus_tag="ISB30_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP1992203.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQM"
     gene            complement(414..489)
                     /locus_tag="ISB30_004653"
     tRNA            complement(414..489)
                     /locus_tag="ISB30_004653"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(454..456),aa:Thr,seq:ggt)
     gene            complement(496..570)
                     /locus_tag="ISB30_004654"
     tRNA            complement(496..570)
                     /locus_tag="ISB30_004654"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(535..537),aa:Gly,seq:tcc)
     gene            complement(687..771)
                     /locus_tag="ISB30_004655"
     tRNA            complement(687..771)
                     /locus_tag="ISB30_004655"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(735..737),aa:Tyr,seq:gta)
     gene            complement(780..855)
                     /locus_tag="ISB30_004656"
     tRNA            complement(780..855)
                     /locus_tag="ISB30_004656"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(820..822),aa:Thr,seq:tgt)
     gene            1217..2167
                     /gene="coaA"
                     /locus_tag="ISB30_004657"
                     /gene_synonym="panK"
     CDS             1217..2167
                     /gene="coaA"
                     /locus_tag="ISB30_004657"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP1992204.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2196..3161)
                     /gene="birA"
                     /locus_tag="ISB30_004658"
     CDS             complement(2196..3161)
                     /gene="birA"
                     /locus_tag="ISB30_004658"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAP1992205.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGESLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNSLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3158..4186)
                     /gene="murB"
                     /locus_tag="ISB30_004659"
     CDS             complement(3158..4186)
                     /gene="murB"
                     /locus_tag="ISB30_004659"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP1992206.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKVLLAQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGRAAVHRQQALVLINEDNAKSEDVVQLALHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1109 a         1053 c          960 g         1156 t
ORIGIN      
        1 tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc cgggcagtct
       61 acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt gagaagtgtt gatggtgata
      121 ccacgagcct tttcttccgg cgcgttatcg atctggtcga atgcgcgagc agcaccgccg
      181 taggttttag ccagtacggt agtgattgca gcggtcagcg ttgttttacc atggtcaacg
      241 tggccgatag taccgacgtt aacgtgcggt tttgtacgtt caaacttttc tttagacatc
      301 gattgtccct ctaagacacg gataaatcgg tgatatcacc acatcaacca ggcaacatgc
      361 ccgacttgtt gaatgcaata aacagaagga aaaaacaggg aggagaaaag aagtggtgct
      421 gataggcaga ttcgaactgc cgacctcacc cttaccaagg gtgcgctcta ccaactgagc
      481 tatatcagca catcttggag cgggcagcgg gaatcgaacc cgcatcatca gcttggaagg
      541 ctgaggtaat agccattata cgatgcccgc atcctggaac tcggctacct gattttcatt
      601 ctgcactgaa tatcgagaga agctctcttt attcgagccg gtaagcgaac ttatcgtctc
      661 gggctacgcc atcgcgtggc cgaaattggt ggtgggggaa ggattcgaac cttcgaagtc
      721 tgtgacggca gatttacagt ctgctccctt tggccgctcg ggaaccccac cggacttgat
      781 ggtgccgact accggaatcg aactggtgac ctactgatta caagtcagtt gctctaccta
      841 ctgagctaag tcggcatcaa gtagcgcgca ttctatggag acatgcgagt tcatgcaact
      901 aaaaaattgc ataatttgtt ttattggtca cattttatgc gacacgatga agaaacagcc
      961 gaacggaggg cagattagca cactttttca acatcattgt gctcaacaat gtgctcctgc
     1021 taaaccataa ttctttttat cagatggaat atctgtcaca ttgcttttca acgatagctt
     1081 cctgggagag attttttctt attattcctc cccatctggt gttaccctcc tgcccattaa
     1141 cccattcaac agaactgtga cgcgccatgg caaatatcgc tttgccgata gagctatgac
     1201 cgccagaaac atgcttatga gtataaaaga gcaaacgtta atgacgcctt acctacagtt
     1261 tgaccgcaac cagtgggcag ctctgcgtga ttccgtacct atgacgttat cggaagatga
     1321 gatcgcccgt ctcaaaggta ttaatgaaga tctctcgtta gaagaagttg ccgagatcta
     1381 tttacctctg tcacgtttgc tgaacttcta tataagctcg aatctgcgcc gtcaggcagt
     1441 tttggaacag tttcttggta ccaacgggca acgcattcct tacattatca gtattgctgg
     1501 tagtgtcgcg gtggggaaaa gtacaaccgc ccgtgtattg caggcgctat taagccgttg
     1561 gccggaacat cgtcgtgttg aactgatcac tacagatggc ttccttcacc ctaatcaggt
     1621 tctgaaagaa cgtggtctga tgaagaagaa aggcttcccg gaatcgtatg atatgcatcg
     1681 cctggtgaag tttgtttccg atctcaaatc cggcgtgcca aacgttacag cccctgttta
     1741 ctcgcatctt atttatgatg tgatcccgga tggagataaa acggttgttc agcctgatat
     1801 tttaattctt gaagggttaa atgtcttaca gagcgggatg gattatccac acgatccaca
     1861 tcatgtattt gtttctgatt ttgtcgattt ttcgatatat gttgatgcac cggaagactt
     1921 acttcagacg tggtatatca accgttttct gaaattccgc gaaggggctt ttacagaccc
     1981 ggattcctat tttcataact acgcgaaatt aactaaagaa gaagctatta agactgccat
     2041 gacattgtgg aaagagatca actggctgaa cttaaagcaa aatattctac ctactcgcga
     2101 gcgtgccagt ttgatcctga cgaaaagtgc taatcatgca gtagaagagg tcagactacg
     2161 caaataattt tcaggggagc taatgctccc ctttcttatt tttctgcact acgcagggat
     2221 atttcaccgc ccatccaggg ttttattatt ccatcctgct caagtaataa agcaccctgt
     2281 ttgtctattc cgcgtgaaat gccaaatatt tctttatcac caatgataag tttcactggg
     2341 cgattaataa aattatccaa cttttcccag cgcgaaagat aaggtgccaa tccttcttgc
     2401 tcgaagagtt ccagcgccgc gcgtaattca cgtatcagca tggccgcaag actattacga
     2461 tcgagattga tcccggcttc ctgcagcgtg atccaccctt gattaacgac actctcttca
     2521 acacgacgca ttgccatgtt gatccctgcg ccaatgacta tttgcgccgc atcgccggtt
     2581 ttgccagtca gctccacaag aatgcctgcc agcttgcgat cctgcagata gaggtcatta
     2641 ggccatttaa cacgaacttt atctgcaccc agcttgcgta atacttccgc catcacgata
     2701 ccgataacca gacttaaacc aatcgccgcc gccgggcctt gttccagacg ccagaacatc
     2761 gacaaatata agtttgcgcc aaaaggcgaa aaccatttcc gaccccggcg accacggcca
     2821 gcctgctggt attctgcaac acaggcatcg cccgatttaa gctctccgat acgatcaaga
     2881 aggtactgat tcgtggagtc aataacgggc agcacagtta cactaccgcc atccagctga
     2941 cccaatatct gttcagcatt aagtaactgg ataggctcag gcaggctgta tcctttaccc
     3001 ggaacggtaa agacatcaac gccccagtca cgcagtgtct gaatgtgttt attaatagcc
     3061 gcccggctca ttcctaacga ttcacccaac tgctcaccag agtgaaattc gccattcgct
     3121 aacagcgcga ttaatttcag cggaacggta ttatccttca tgaaatcgtc tccactgcgc
     3181 taacttcacc tgatgcacca ataaagcgaa cttcaggctc aagccagaca ttaaattttt
     3241 cacccacttt ttgtcttaca tgaagtgcca gttgcaccac atcttcgctt tttgcattgt
     3301 cttcattaat gaggactaac gcctgttgac ggtgcaccgc agccctaccc atttgcatcc
     3361 cttttagctg gcactgatcg ataagccaac ctgctgccag ttttactgaa ccatccgcct
     3421 ggggataatt tggtgctgtt ggaaattgtg ccagtaatac tttagccgtt tcggcagata
     3481 caacagggtt tttgaagaaa ctaccggcat tgccattcac ttttggatca gggagtttgg
     3541 tggtgcgcat atgacacacc gcattaaata cttgctgtgg cgttactgtt gtaggatcca
     3601 gacgagttaa gtcaccatac gtcagtacag gttgccactc ttttggcaga cgcaggccta
     3661 cggctacaat agcgaagcga tcctggtatt catgtttaaa aatactgtcg cgatagccaa
     3721 aacggcactc tttggcagtt aagcgcactt gcttgcctgt cgccagttca acacaatcaa
     3781 cataagcgca aactcgctgt aattctacgc cataagcacc aatattctgg ataggtgatg
     3841 agccgacaca accaggaatt aatgccagat tttccagacc aggcatacct tcctgcaaag
     3901 tgtattttac aaggcgatgc cagttttctc cggctcctac atgtaaatac cacgcatcag
     3961 gttcatcatg aatttcgata cctttgatcc ggttgatgat caccgtgccg cgatagtctt
     4021 ccagaaaaag tacattactt ccttcaccca gaataagaac gggttgtcct tctgcggttg
     4081 catgctgcca ggcattgagt aattgttgtt cgtcttcggc acatacaatg tgctgagcat
     4141 tatgatcaat gccaaatgtg ttccagggtt ttaaggagtg gttcatagct gctttcctga
     4201 tgcaaaaacg aggctagttt accgtatctg tggggggatg gcttgtagat atgacgacag
     4261 gaagagttta tagaaacg
//
LOCUS       DACADF010000251         4242 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.253, whole genome shotgun sequence.
ACCESSION   DACADF010000251 DACADF010000000
VERSION     DACADF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.253"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            53..847
                     /locus_tag="ISB30_004660"
     CDS             53..847
                     /locus_tag="ISB30_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1992207.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLARLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLRTLSDFRLLYTGCGDSRVKRNIRLHAKYIGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNISKKRPGLL"
     gene            1001..1762
                     /locus_tag="ISB30_004661"
     CDS             1001..1762
                     /locus_tag="ISB30_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992208.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYLDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAENLPVMDCYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNNFHGDGNA
                     LETTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1804..2880
                     /locus_tag="ISB30_004662"
                     /pseudo
     CDS             1804..2880
                     /locus_tag="ISB30_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2902..4095
                     /locus_tag="ISB30_004663"
     CDS             2902..4095
                     /locus_tag="ISB30_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1992209.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLSVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSV
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNATVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
BASE COUNT         1158 a          921 c         1072 g         1091 t
ORIGIN      
        1 acattttgaa tcaaaatgtc aggttttgta gcaatggcgt ggacgagtct gaatgaagaa
       61 gcctctggtt attatcagtg cctgtcagtt caccaggctg gcactggagt ctctcatccc
      121 aacggacagg tatatcgtgc gggtgtacag caacgtcact acagaggttg aattcgtttt
      181 aaagacctcc tgtggctacc tgttagctga tatcccttcg cttccttcgg gggagttggt
      241 tcttctggct tgtctcgccc gtttgcggag tgatgccggg caatggcagg tctgcctgac
      301 cggtgactcc gactggttcg ataatgctcc tgctgcaagt ctgtataacg gcttcccacg
      361 catcggcacc atgctgacag cgagtcgtta tcagaatcaa attatccgct ggttactgcg
      421 tcctttgccc ggcgatatga gcgatttctg gctcctgact gcacgggagt tgagcgtgct
      481 taagattctg atgcagggca tgagtttcag tgagattgcc cgttatgaac aacgcagttc
      541 aaagaccctg catgcgattg ccacacgggc tttaatgaaa cttggactga gaacactgag
      601 cgatttcagg ctgctctata cgggatgcgg tgacagcaga gtgaaacgga atatacggct
      661 acatgcaaaa tatatcggac aacattccgc tcatcgactc actcagtatg tgcagaacat
      721 ggtatccgga atcctttatc cagagaaagt tgccgttggt gaatcaataa attctgacgc
      781 catttccgga aaagtacttt ccggacaaag aaaaaatata tctaaaaaaa gaccaggatt
      841 gttatgacac agcagcgttg ttactaccac ccactgcggc gcacacggac tcgctacagt
      901 gtaggagcca aaatatttct actgttctgg gctgcaggat tgggaggcat gctgtacagc
      961 ctgtacagcg tgctggcgta ctgacatctg tgagggaagg atgcgtttcg ttcttttctg
     1021 tccctcagga attgtaccag cgcagttcgc agctctatca accggttctg tcggcaatgt
     1081 tacctgcata agaacagaag aggagttacg caataaattg cggcacaggc cacagagtgt
     1141 cgtgatatcg gcagggcgac ctgccgaatg cgctgaaatg tggttccgat tttatctgga
     1201 tcactcattc gtggtggtgt tgtgcgtcgc gccctttttt ctgccaccgg atgtgagtat
     1261 ttctggcgtt ctcaaaaacc tgaggttact gaaaccagga atgtcagtag agcgtgtcat
     1321 cagtattgca aatgcctctg gtgggttttc aggactgaaa catgcagaaa atttgccagt
     1381 tatggactgt tactctgtct ttatgaaaga ggtgaataac cgcacaaaga caatcgtcat
     1441 gtcagaacgc ttcccggaaa agcaaaagaa ggtgttgtcg ttgcttcttg ccggacacag
     1501 ctgggaatac agtgcgcagt ttctcaaaac ggggataaga cagatatggc ttgccgagca
     1561 gtcgctaaaa aagcgttggg ggataccgga cagcatgtca ttgcgggaag ccctgctgct
     1621 gagcagcaat aattttcacg gtgatggaaa tgcattggaa acaacgaatg ccatgacatt
     1681 gcgtgaaaac ggaaatacca acaggtattc cgtggtggta aatgcaggga cgcaggcttt
     1741 atctctgcat aaatataaat aagtgggaaa ttgaaattag ttaccagaat ggagaatttc
     1801 ttcatgaaaa acagtaaggc attttaccgc agcgcattag cgacagctat tgttatggct
     1861 ctttctgcac cagcattcgc tgctgataac gcggtatcaa ctacaccggt tacattggat
     1921 aaagataaga caactctgga acaagatgtt gttattagtg gtactacaca gattacagcc
     1981 gcaacaatta atgcgaaaga taaagacctt aatgttactt tgggtggtca tgatattacc
     2041 gccacatcaa cggtagacca aggtttcgtt gaaggtgtaa aagttagcgg tgacaaaaat
     2101 gttgtgatta atgctacagg ctccaccatc acagctcaag gtgaaggtac ctatgtccga
     2161 actgcaatgg tcattgattc aactggcgat gttgttgtta atggcggtaa ctttgttgca
     2221 aaaaatgaaa aaagtagtgc gacaggtata tctctggaag cgaccacggg aaataattta
     2281 acgctcaatg gcacaaccat aaatgctcaa ggtaataaga gttccagcaa cggctctacg
     2341 gcaatttttg ctcaaaaggg taatttgttg cagggttttg acggtgatgc aaccgacaac
     2401 atcacccttg ctgactcaaa tattattaat ggtgggattg aaacaatagt tattgccaag
     2461 gagaataagg gaattcatac agtcaacctg aatattaagg atggctcaat aattggggcg
     2521 gctaataata aacaaacaat ttatgcctct gcttcggcac aaggcgcagg ttcagcaacg
     2581 caaaatttaa atttgtctgt tgctgattca accatctact ctgatgtcct ggccctttct
     2641 gaaagcgaga attcagccgg taccacaaca aatgtaaata tgaacgttgc ccgctcttac
     2701 tgggaaggta atgcttatac gctcaatagc ggcgataaag cgggtagtga tctggatata
     2761 aatctttccg atagttcagt ctggaaaggc aaagtttcag gggcaggaga tgccagtgta
     2821 tctctgcaaa acgggtctgt ctggaatgtt acgggttcct caactgttga tgctctggca
     2881 gtaaaagaca gtacggttaa tatcacgaag gctacagtca atactggcac gtttgcttct
     2941 caaaacggca ctctgattgt tgatgcctct tctgaaaaca ctctggatat cagcggaaaa
     3001 gcgagcggtg acttgagtgt ttacagtgcg ggttcattgg atcttatcaa tgaacaaacg
     3061 gcatttattt ctaccggcaa agacagcact ctaaaagcca caggcacaac ggaaggtggt
     3121 ctgtatcaat atgacctgac acagggagct gatggtaact tttatttcgt aaaaaacacg
     3181 cataaagcat ccaacgccag ctccgtgatt caggcaatgg cagctgctcc ggctaacgtc
     3241 gctaatctgc aggctgacac gctctccgcc cgtcaggatg ctgtccgtct gagcgaaaat
     3301 gacaagggtg gcgtatggat tcagtacttt ggcggtaaac agaaacatac caccgcggga
     3361 aatgcatcct atgacctgga tgtaaatggt gtaatgctgg gtggtgatac ccgcttcatg
     3421 actgaagatg gtagctggct ggccggtgtg gcgatgtctt ctgcgaaagg tgacatgact
     3481 accatgcaga gcaaaggtga cactgaaggt tacagcttcc acgcttacct gagccgccag
     3541 tataacaacg gtatcttcat tgatactgct gcacagtttg gtcactacag caacacggca
     3601 gatgttcgcc tgatgaatgg tggcggtacc atcaaagctg actttaacac caatggtttt
     3661 ggtgcgatgg ttaaaggcgg ttacacatgg aaagacggta atggcctgtt tattcagcca
     3721 tatgccaaac tgtctgctct gactctggaa ggtgtggatt atcaactcaa cggcgtggac
     3781 gttcattctg acagctataa ctctgtgctg ggtgaggccg gtacgcgcgt gggttatgac
     3841 ttcgctgtgg gcaacgcgac cgttaaacct tatctgaatc tggccgcact gaacgaattc
     3901 tctgatggca acaaagtccg tctgggtgat gagtctgtca atgccagcat tgacggtgca
     3961 gcattccgcg tgggtgcagg tgtacaggct gatatcacca aaaacatggg agcatatgca
     4021 agccttgact acaccaaagg tgacgacatt gagaacccgc tacagggtgt agttggtatc
     4081 aatgtgacct ggtaagaata aagcagtcgg ttcccctgtc gactgttata ccccctgcag
     4141 gcagcagtat gctgcctgca ggctataaat atccagtact catgatgctg gctttttgct
     4201 gatatttacc ctgaacattc ctgatacaga ctattaatac aa
//
LOCUS       DACADF010000252         4148 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.255, whole genome shotgun sequence.
ACCESSION   DACADF010000252 DACADF010000000
VERSION     DACADF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.255"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..1764
                     /locus_tag="ISB30_004664"
     CDS             <1..1764
                     /locus_tag="ISB30_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HAP1992210.1"
                     /translation="LTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTLY
                     QNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVTA
                     RREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLSG
                     ILDQLTLMADARQSPLIALTDTIAWQVATGRENRGLSDSLAKSAQELFNGKEKTPQQS
                     REGDVPAGPLDKTFAPLLRLLGDKSGGGGGDTQLNLQTYLTRVTRVRLKLQQVTNAPD
                     PQEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVL
                     TPAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAAN
                     LSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKP
                     ARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRG
                     SWSLIRLLERAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPG
                     QVFLTGRSMKDAEEYGEDADE"
     gene            1757..3367
                     /gene="tssA"
                     /locus_tag="ISB30_004665"
     CDS             1757..3367
                     /gene="tssA"
                     /locus_tag="ISB30_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HAP1992211.1"
                     /translation="MSELLQKLTRSCFADRDALDVARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTGLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLAGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALNQLSVSFTAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALAKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGITLTQWEPELLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVAIDPVRAAVLCDTQHKD"
     gene            3373..>4148
                     /locus_tag="ISB30_004666"
     CDS             3373..>4148
                     /locus_tag="ISB30_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HAP1992212.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGESEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMINGGSTKNTEKIDF
                     TITNKGDEAEGLSLEVLDGNEVIYSERQTGKYFTKGEHAWQWDGYSNQGILDTTKLKS
                     KSLLVRLIALCGDMMIKVDYPLKTSANEEDWVDIEVNKNN"
BASE COUNT          999 a         1100 c         1282 g          767 t
ORIGIN      
        1 ctgaccttct ggatggcgaa cctgccggaa cacccgcagt ggaaaacctc accgccaccg
       61 gaactgaccg gcgcggtgcg taaaatcctg ctgcgccaga ttggtgtgcg taatgccgaa
      121 aacaccctct accagaacgt gctgcaacag gtgtcccgca actacgccga tatgacgctg
      181 gcggacatga ccggggatac cctcactgaa tctcttttca gtacggaaca gacggtgccg
      241 gggatgttca cccgtcaggc atgggaagga caggtcaggg aagctatcga gcaggtggtg
      301 acggcgcggc gcgaggaaat cgactgggta ctcagcgacc gccagcagga tacctctgct
      361 gatatctcgc cggatacgct gcgtaaccgc ctcacctcac gctactttac cgactttgcc
      421 ggaagctggc tggcgtttct caacagcatt cgctggaaaa aggaagactc gctctccggc
      481 attctcgacc agctgacgct gatggccgat gcccgtcagt cgccactgat tgcactgaca
      541 gacactattg cgtggcaggt ggcaacaggc agggaaaatc gtggcctgtc agactcgctg
      601 gcgaaatcgg cacaggaact gtttaacggc aaggagaaaa cgccgcagca atcccgtgaa
      661 ggcgacgtgc ctgccgggcc gctggataaa accttcgcgc cgctgctgcg gttgctgggc
      721 gataagtcag gaggcggtgg tggtgatacg caactcaacc tgcaaacgta tctgacccgc
      781 gtcacccgcg tacgcctcaa actgcaacag gtgaccaacg cccccgaccc gcaggagatg
      841 acccagcaac tggcgcagac ggttttacag ggcaaaaccg ttgacctcac cgacacccgc
      901 gactacgggc ggttaatcgc cgccagtctg ggcgaagaat ggagtggctt cggtcaggcg
      961 ctgttcgtcc gcccggtgga acagtcgtgg cggcaggtgc tgacgcccgc agcggacagc
     1021 ctgaaccgcc agtggcaacg ggcgattgtc agtcactgga atcaggactt cgctggccgc
     1081 tatccgttca aagcctcaca gaacgatgcc tctcttccct tgctggcgca gtacctgcgc
     1141 gatgacggac gcatcaacca gtttatcgcc gccaaccttt ccggtgttct gaaacgtgaa
     1201 ggccgctact gggtggctga cgccatgaac acgcaggggc tgacggtcaa cccggacttt
     1261 atccgcgccc tgaaccgcct gcgcgatgtg gccgataccg cctttgccag cggcgatgcc
     1321 ggaatacatt tcgaactgcg ggcaaaaccg gcgcgtgacg tgatgaaaac gcatctggtg
     1381 attgacgggc aggaactgga gtatttcaac cagaaagaac gctggcagcg ttttaactgg
     1441 ccggatgaac agtggcaacc cggcgcatcg ctgagctgga ccagcacaca ggcgatggaa
     1501 cgcatactgg cggattaccg gggaagctgg agccttattc gcctgctgga acgggcgcag
     1561 gtaacaccgg tcgacagcag cacctttaaa gtggtgtgga aagcgcagga cggcctgcca
     1621 ctgaattacc tgctgcgggt tgaacagggt aaagggccgc tggcgctgct ggaactgaaa
     1681 aacttccgcc tgccgggaca ggtatttctg accggcagga gtatgaagga tgcggaagag
     1741 tatggggaag acgccgatga gtgaactgtt gcaaaagctg acccgtagct gttttgccga
     1801 ccgggatgca ctggatgtgg cgcgaacaca ggcggcgctg tggcagacat ggctgctgcc
     1861 ggtgacggca gacacgccgg tcggggaaga ccccggctac cacgacgatt tcctgcgtat
     1921 ccgcgacgaa atgaacaaac tgtccggtgc cgataccggc cttatctgcc agcttgccga
     1981 atccctgctg ctgacgcagg ccaaagacgt gcgcatcgcc acttactaca tctgggcgcg
     2041 actgcaccgc gacggtgagc gcggactggc tgaaggactg gcgctgttag ccgggctggt
     2101 ggaacgcttc ggtacgcaac tgctgccctc gcgaccggca agccgaaaaa tggcgctgga
     2161 gtggctggcg ggggaaaaga tgctcgacag ccttgcccgc tacccggagg tggcaaaaga
     2221 ggattttgcg aacattgttg ccgctcttaa tcaactgtcg gtttccttca ctgcatggcc
     2281 cgaagaccag cattccccct ccctgatgcc gctgattaac gcgctggaat cacgtctggc
     2341 gcagtcgggc gggatgaacg cggtggtgcc gcaaaacagc agcggcgttc cggcaccgtc
     2401 ttccccggtg gatgcgccgc aggtacagac catcacctcc gggcgcgacc tactcgacca
     2461 ggcgaaagtg ctggcccgtt atctgaacga acagccgcag ggctggcttt cagcacaccg
     2521 gctgatgaaa accctgcgct gggacacggt gcatgaactg ccgccggacg tggacggcaa
     2581 aacccggctg gccccgccgc gcaccgaatc acgcaaccag cttaagcggc tgtatgccca
     2641 gcagaactgg acggaactgc tggagcaggc cgacctgatg ttcagcaccg gtgtcagcca
     2701 tttctggctg gatatccagt ggtatctgca tcaggcgctg gcgaaggcgg gcgcaccgtg
     2761 ggacagatgg acagcggtta tccgtcagga cctggcgctg ctgctggagc gcctgccggg
     2821 gctggaaaat ctggcatgga acgacggcac gccgtttgcc gatgaggtga cgcgcaactg
     2881 gatagcgcag caggtcatga tgcgggaaga cggcgcatgg cttgcgggga aagccgctgt
     2941 gccgacagac gacgccacaa atgacgtact ggcgctggag ccggaagcac tggagatggc
     3001 tgacagtcag ggcgtggaag cggcgctggg ctggatacag acccgtccgg gcatcaccac
     3061 ggcacgacag cgcctgttat tgcgactgct gatggcacgg gtggcggaac agtacggtaa
     3121 aaacgagatg gcgctcctgc tgctggaaga actggatacc gccgcacagg gaataacgct
     3181 gacacagtgg gagccagagc tgctgtttga agtaaaagcc cgacaactga aactgctgcg
     3241 tctgcgggcg caccgctatg cggataaagc cctgctgaac cgaaaaatgg aaatcctgct
     3301 gggaacgctg gtggcaatcg atccggtacg ggcagcggta ttgtgtgata cgcaacataa
     3361 ggactgatgg caatgggaac aggctacttt ctggtcaggg gggataagac cacctgtggt
     3421 ggaaaaatta ttgaaggggc ggacgaccat acgatcatgg gtatcccgca ggcacgggat
     3481 atggaccggg tcacctgcgg cagatatccg ggaatgttta ttattgtggg cggcgttcct
     3541 gaaacagata ttcacggcag gctgatggcg gggacgctgg acagtcagag cagttgcccg
     3601 tgtaaagcac ggtttattgc gtcaatgatg gatgatacgt atgagacgga tgacggggaa
     3661 agtgaagcgg aacaacatgc gcagtcggcg aaaaaggatc tgacttctgg ttctgattca
     3721 tcctctgatg atgtaaaact ggattacaga attaaattat caggtaataa gatactgacg
     3781 ccactgaata tacctgatta taaagaaatg ataaacggtg gtagcacgaa gaatacagaa
     3841 aaaatagatt ttaccatcac aaataaaggc gatgaggctg aaggactttc cctggaagtg
     3901 cttgatggca atgaagtgat ttatagtgaa cggcagaccg gtaaatattt tactaaggga
     3961 gagcatgcct ggcaatggga tggctacagt aatcaaggca tactggatac aactaagcta
     4021 aaaagtaaat cactactcgt aagactgatt gctttatgtg gtgatatgat gataaaagtt
     4081 gattatccat tgaaaacttc tgcgaatgag gaagattggg tagatataga agttaacaaa
     4141 aacaataa
//
LOCUS       DACADF010000253         4108 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.256, whole genome shotgun sequence.
ACCESSION   DACADF010000253 DACADF010000000
VERSION     DACADF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.256"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            61..825
                     /locus_tag="ISB30_004667"
     CDS             61..825
                     /locus_tag="ISB30_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002008502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HAP1992213.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(1318..1902)
                     /locus_tag="ISB30_004668"
     CDS             complement(1318..1902)
                     /locus_tag="ISB30_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1992214.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(1902..3140)
                     /locus_tag="ISB30_004669"
     CDS             complement(1902..3140)
                     /locus_tag="ISB30_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1992215.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(3137..4042)
                     /gene="mph(A)"
                     /locus_tag="ISB30_004670"
     CDS             complement(3137..4042)
                     /gene="mph(A)"
                     /locus_tag="ISB30_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HAP1992216.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT          850 a         1285 c         1285 g          688 t
ORIGIN      
        1 gctctgttgc aaaaatcgtg aagcttgagc atgcttggcg gagattggac ggacggaacg
       61 atgacggatt tcaagtggcg ccatttccag ggtgatgtga tcctgtgggc ggtgcgctgg
      121 tattgtcgct atccgatcag ctatcgcgac cttgaggaaa tgctggcgga acgcggcatt
      181 tcggtcgacc atacgacgat ctatcgctgg gtccagtgct acgccccgga gatggagaag
      241 cggctgcgct ggttctggcg gcgtggcttt gatccgagct ggcgcctgga tgaaacctac
      301 gtcaaggtgc ggggcaagtg gacctacctg taccgggcag tcgacaagcg gggcgacacg
      361 atcgatttct acctgtcgcc gacccgcagc gccaaggcag cgaagcggtt cctgggcaag
      421 gccctgcgag gcctgaagca ctgggaaaag cctgccacgc tcaataccga caaagcgccg
      481 agctatggtg cagcgatcac cgaattgaag cgcgaaggaa agctggaccg ggagacggcc
      541 caccggcagg tgaagtatct caataacgtg atcgaggccg atcacggaaa gctcaagata
      601 ctgatcaagc cggtgcgcgg tttcaaatcg atccccacgg cctatgccac gatcaaggga
      661 ttcgaagtca tgcgagccct gcgcaaagga caggctcgcc cctggtgcct gcagcccggc
      721 atcaggggcg aggtgcgcct tgtggagaga gcttttggca ttgggccctc ggcgctgacg
      781 gaggccatgg gcatgctcaa ccaccatttc gcagcagccg cctgatcggc gcagagcgac
      841 agcctacctc tgactgccgc caatctttgc aacagagccc gccgtgctag tctgctcggt
      901 gatggtggag tgaagccaac ccgcaatcgg gttatgaatc tgcatcgcga ttcgcaatca
      961 gctgtctctt gagcatgtcg aactcctgcg atgttatctc gccgtgctcg tgctgctaca
     1021 ccaacttgtc caactcatcc gccacaccgc tccccaccac ctgcgatggc caggttgttt
     1081 gctgggcgca ttggcggagt atcgtctcga cccgggacac ccacaccggc acaaccttac
     1141 gggagtgatt cactgtcaaa gaatcggccc ggtgctctga cgcaagtatc gggatggtca
     1201 ccatttgtaa gccgtagacc tgagtggtga tcaagacttc gataccaccg accgtaccgg
     1261 tactaatcga cgacggtcgt gttcgtcgcc tgccgcaggg actctgcaca cctccgttta
     1321 cgcatgtgcc tggaggagtt ggaaatcgtc gtgttcggga aacattaaac acaggatggc
     1381 agcgatctga gccagcacat gatcagctag ctcaccatcc ggatcgacgg cccactgcat
     1441 cgtcgcgcca gcgatgaccg agtgcaggag caactcagct gccgcaggag cacctggggg
     1501 cagtcgcttg cggatcccct ccaccaccgc gcggttccgc tggatcgcaa gcgtgcgtag
     1561 ctccggcacc tggagctcgt accaggagat gagatagttc accgagaagt cgttgcgagt
     1621 gttcatgctc cgaacgagca cctgcaaaaa ttcccagagc ccttgcggcc ctgcgcctat
     1681 cggtatcgca ttcaggtaat gccgcacctg ctcgacgccg cgctccatca tcctcaccag
     1741 cagcgtatcg cggttggtga agcgctggat taacgctgcg cgggagagcc ccacctcctt
     1801 tgctactccg ctgagcgtga actctatggg accgcaacgc ttcagcacta cggtggcggc
     1861 ctcgagtacc tcgtcatcgg acttgagctt ggggcggggc atcagtgttc accttctgta
     1921 tgggttgggg cggaggctgt ggctgccgcc gccattgtag caaattgaag acggagcgag
     1981 agtagagcca cgagccccgc aaacacggcc gatacaacga ggccagggag cggaccagca
     2041 aggtcgacaa acgcgccggc cgcaagcata accatgggcg aggctgacag catcaccgcc
     2101 gagaccgtgc cgagtacccg gccgagaagt tctggcggcg tgcggttgta gatggcagcg
     2161 ttgagaatgg gagagactga gccggtcagc agtcccacga gcgcgcccaa caacatcagc
     2221 accggcacgc ctggcaactg tgaaagcaga agcgagccca ccgcagagcc acaaaatgcc
     2281 accgccagcc agttctgcgc tgatatccgg gcgccgaccg acgcatgaat ggcaatgcca
     2341 aggagaccac cagcccccat cattgaggag aacagcccga gctctgctac ttggcgtcct
     2401 gcatctacaa acagcgcagg catgatgacg ctgccgttgg cgccaacgat gcccacgaag
     2461 atcatcacta taccaaagag agggcgcagc aggggttcgc tccagagaaa agcgacgccg
     2521 gcgcgcatgg agagagtcgc cgtcgtggtc atcgtccgag cggcacgcgc gggaagcacc
     2581 cacgcgccga gcagacctgc aaggacggag cagaacgccg tcagcccgag cgttggcgca
     2641 gcgccaagca ggccgattgc ggccccccca agggccgggc cacctagaat cgcgacgttc
     2701 ccgatcaccg ctttcagtga cgagacgcgc tcaacggaga gcccggcgac gtggccgagt
     2761 ttgggcagct cactgtcctg cgcggccata ccgggtgcgt cgaacgcggc accgagcacc
     2821 acgcaagcga tcagcccagt gttcgagagg gcgccaacgg catcgagcag tgggatgctc
     2881 gccatggcca cgccgcccac cacacccgag atcaatgcga cgggcgcgcg cccgaaccga
     2941 tcgacgaggc caccaccaac ccacgcgccg atgatggtcg cgatgacgct gctagcggcc
     3001 gtggcgcccg cccaggccgc gctctttgta tgagacagga cgaaccatgg aagcgcgagg
     3061 gccgccaccg cgttgccgat ccggaagaga aaggtcgccg cgaacagcgt cgcgagcggg
     3121 ctatatcgac gttcgctcat tccgctgcgg cgagctgcgc cttcgccgca gcgaggtact
     3181 cttcgttacc cgagtcgagg gcgaagagtg cgtaggtgac cgccccgaac gcaaggcgct
     3241 ccgcgatgtg gtgggcgagc cgcggccaca cccggccacc ggccgcttca tacgtgagga
     3301 ggagcttcgc gagcccctct tcaccaaaga ccataaggtg cgcggccatg tcgatggcag
     3361 ggtcatcaac gcgggcctcg ctccagtcga tcatcccgct gacgcgctcc gtgttgtcga
     3421 tgagcacatg gcccacgtag agatcgccat gcaccaccac ggagaaatct ggccacgacg
     3481 aatcgtcgtc gagccagcgc tgccaccggt ggaggcgctt gtcgttcacc acgaactcgc
     3541 gtcggacgcg gtcaacgtcg tcggccacct tctgacgggc ctgcgtcggt gtacggatga
     3601 gcatccccgc atccacggcg gcggaaatgg ggacggcatg cagggcggcg agcgcggtcg
     3661 cgaagctctc cgcgaagacc tccgagtcct gcggcacgac ccagtcgggc gtggacgaac
     3721 caggctggat gaccatcgca gtcgagtctt cgagcatggg ataggcaacg agctcggcgt
     3781 tggccacgcg ccagtccggc accgcgaacg gcaggcgatt cttgagcatt gccagcaccc
     3841 gcgcctctgg ttcgaccttc gcgcttacct cggctcggcg cgggatgcgc agcacccacc
     3901 gacgtccatc gtcgacggtg gcgatcacga tcctatagtc gagcccaagc tcattgacag
     3961 tcagcgggcc atggagcttg agcccatgtc gggctgcaag tgcgtacagt tgggaggtat
     4021 cggcggtcgt gactacggtc atgattcact cctgagggct tgacgggttt agccacctaa
     4081 atgtaacagt cacgtcggtt atattcaa
//
LOCUS       DACADF010000254         4100 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.257, whole genome shotgun sequence.
ACCESSION   DACADF010000254 DACADF010000000
VERSION     DACADF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.257"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            7..963
                     /locus_tag="ISB30_004671"
     CDS             7..963
                     /locus_tag="ISB30_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HAP1992217.1"
                     /translation="MTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNL
                     GHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQVREMADIISELNSL
                     SDKIQKLSDDAATANADPADMAAQIALITSRINDLTASVILMHAPKGVAVASGEHLQL
                     AAVKNLQINAGNNADIGVVKNMFIGVGRALSVFVRKAGIRLIANKGAVSVQAQHDLME
                     LLAKKSIEIVSTEDEIKITAKKKITINGGGSYIRIEGSGIEPGTPGDYNVKAVHYGRQ
                     PKASEKVPMPEFPILSAVDSSDFCLECLLNAIKNDDAVVEGV"
     gene            966..1787
                     /locus_tag="ISB30_004672"
     CDS             966..1787
                     /locus_tag="ISB30_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HAP1992218.1"
                     /translation="MSSEQQLADHWLKRNETLNAFILFEGGALSQEMLQIMMEHYKYR
                     RISLYQLPDLKKVAAYGPWLVRIESEKDLAMVLVGKLPVAGITFSELQLEPLASRLAV
                     GCMAKTPDGREVLLRYYTPRVLKQLAARPDMDWHPVMFSQIESWWMLEDTRWQRLNMP
                     LTVANNSDETVEIITLDEPLWQKIADRGDVSALLREWRTMKTSEHFPPCIQRNMVEKA
                     LNKAQVVGLTKPLDQKIYALSYLNGKKAYLESAEFQVMLEKVKRNEISLSDLGRQ"
     gene            1831..2358
                     /locus_tag="ISB30_004673"
     CDS             1831..2358
                     /locus_tag="ISB30_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HAP1992219.1"
                     /translation="MGLFTRLEKFDQKLTRGYARWGRWVWRMLIAIPVAYFLLCVGIS
                     IWGAPTGGVILVIHSELDRPILGFSVNGMAGANAFAHGGGSTTCCGDIRGKEAEVVWT
                     LSTTRAQYNTGLREEVRRITLPLPERKHGQDFLHVHFLPGDKVFLGWSEGAGSPYEKR
                     KEPSYPSRKNQEAQP"
     gene            2355..>4100
                     /locus_tag="ISB30_004674"
     CDS             2355..>4100
                     /locus_tag="ISB30_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HAP1992220.1"
                     /translation="MISESVISAAVRAQQSEDGKVGNCGRIWHVAFFFDGVGRNIDRD
                     ATDCRLSNIARLFRAYPDEQKNTGDTCYNKFYFSGMGTAYHDDSVDNIHALMDIGLDN
                     FLEDMKSLPEDTISDAGESVIKGEKKWTDVLGNLLEDLNNPVEWVKGIGKMAAKSVGK
                     AGIESTPWLRDSEAMSAYFMTGEPVRLEAAKRLFKKFYTENMKGDVPIKKISVSLYGF
                     DLGATLARKFLDNFLKEVCLKNKEKKHQYKNALVDIAFIGLFDCSRHSPTSSNNGLDY
                     FFMWLGLPGKMIGSVLGEKAIDQDSPLPDVVNNALHLVAAHERRPWRGLYLLGKKTDT
                     ANKKYREELLPGCSEDIGGGLKPDEQKPSAELCRVALYNMYHAACQAGVPFPDFDTLD
                     QYDTKVASYFIMNDAVQGGSVNYWVSRYQREEVKDKVFSEAMQEKYLDNYFLWLGEQY
                     YMYQYERERLDKELTSAHRGKISDYGPLAGTGINPNTNADDIKSQISELDSLWGWLND
                     VKSVAIGLRNDFIVNPRKNDSRIKLKPQVYNAAVNRAVKFLEFSRAVYNDKNLPSSSS
                     LTANTLYSFFVHDIQK"
BASE COUNT         1170 a          788 c         1113 g         1029 t
ORIGIN      
        1 gaccatgtga ccattcagaa ctacaagcgc aacgtgctac gcaccccggc gaacaacaaa
       61 atccgcctcg acgatgagcg cggcaaagag catatcaagg tcagtaccga gtacggcggc
      121 aagagccagc tcaacctcgg tcatctggtg gatgccggga aacagcagcg cggggaaggt
      181 tttgaactgc gtaccgatct gtggggtgca gtacgggcaa agaaaggcat ctttatcagc
      241 gccgacgcgc aggacaaggc gcagggtcag gtgcgcgaaa tggccgacat tatcagcgaa
      301 ctgaacagcc tgagcgataa aatccagaaa ctgtctgacg atgcggccac ggcgaacgcc
      361 gatccggcgg atatggccgc gcagatagca ttgataacca gccggataaa tgacctgacc
      421 gcctcggtga ttttgatgca cgcaccgaaa ggcgtggcgg tcgccagcgg cgagcacctg
      481 caactggccg cggtgaaaaa cctccagata aatgcgggaa acaacgccga tatcggcgta
      541 gtgaagaata tgtttatcgg cgtgggacgg gcgctgagcg tgtttgtacg caaggcgggt
      601 atcaggctga ttgccaataa aggcgcagta tcggtacagg cgcagcacga tctgatggag
      661 ttactggcga aaaaatcgat agagatcgtc agtaccgaag atgagattaa aatcaccgcg
      721 aagaaaaaaa taacgattaa tggtggtggg agttatatcc ggatagaggg tagcggcatt
      781 gagccgggaa cgccggggga ttataacgtc aaagcggtac attacgggcg tcagcctaaa
      841 gcctcggaga aggttcctat gccagaattt cccatccttt cagccgtgga ctcatccgat
      901 ttttgtcttg aatgcctgct gaatgccatc aaaaacgatg acgctgttgt tgaaggagtg
      961 taatcatgtc ttcagaacaa caactggcag accactggct taagcgaaac gaaacgttaa
     1021 acgcctttat tttatttgaa ggtggagcgc tctctcagga aatgcttcag atcatgatgg
     1081 agcattataa ataccgacga atttccctgt atcaactgcc cgatcttaaa aaggtagcag
     1141 cttacggtcc ctggttggtt cgtattgaaa gtgaaaagga tctggcgatg gtgcttgtcg
     1201 ggaaactacc tgtcgcaggc attactttct ccgaattgca gctggagcca ctggcgtctc
     1261 ggcttgctgt gggatgtatg gcaaaaacgc cggatggtcg tgaagttctg ctgaggtact
     1321 acacgccccg agtgctgaaa caactggctg cccggcctga tatggactgg catccggtaa
     1381 tgttttctca gatagaatca tggtggatgc tggaagatac gcggtggcaa aggctgaaca
     1441 tgccgcttac tgtggcaaat aacagcgatg agacagtgga aatcataacg cttgatgaac
     1501 cgctctggca gaaaatagcg gatcggggag atgtatctgc gcttttaaga gagtggcgca
     1561 ccatgaaaac gagtgagcac ttcccgccct gtattcagcg aaatatggtg gagaaggcct
     1621 tgaataaggc tcaggtggtc ggattgacaa agccactgga tcagaaaatt tatgcgttga
     1681 gctatctgaa tggaaagaaa gcgtatcttg agtcagccga atttcaggtc atgctggaaa
     1741 aggtgaaacg aaatgaaata tcgctgtctg accttggtcg ccagtgacat acatctttgc
     1801 caaccaattg ataaaaggga ataaaatctg atggggctat ttacccgact ggaaaaattt
     1861 gaccaaaagc ttacgcgtgg ttatgcccgc tgggggcgtt gggtgtggcg gatgctaata
     1921 gcgatccccg tggcttattt tttactgtgt gtaggaatat caatctgggg agctccaact
     1981 ggaggagtca tattggtgat ccatagtgaa ctggataggc caatattagg attcagcgtg
     2041 aacggcatgg ctggggcaaa tgcgtttgca catggtggag gttcaacgac ttgctgcggt
     2101 gacatcagag ggaaagaagc tgaggtcgtc tggacattga gtacgactcg cgctcagtat
     2161 aatacgggac tgcgtgaaga agttcgacgt atcactctgc ctttgcctga aaggaaacat
     2221 gggcaggatt ttttgcatgt acatttcttg cctggtgata aggttttttt gggttggagt
     2281 gaaggagctg gttcgcccta tgagaaacga aaagaacctt cttatccgag tcgaaaaaat
     2341 caggaggcgc agccgtgata agtgaatcag tgatttccgc agctgtacga gcacaacagt
     2401 cagaagatgg taaagtggga aattgtggcc ggatctggca tgtcgctttc ttttttgatg
     2461 gagtgggacg aaatatcgac cgggatgcga cagactgccg tttgagtaat attgcccgcc
     2521 tgttccgggc atatcctgat gaacaaaaaa atacaggtga tacttgttat aataaatttt
     2581 atttctccgg aatgggcact gcctaccatg acgattctgt cgacaatatt cacgctctta
     2641 tggatattgg tttagataac tttctggagg atatgaaaag tctgccagag gatactatat
     2701 cggatgcagg ggagtctgtt attaaaggag aaaagaaatg gacggatgtg cttggtaatc
     2761 tccttgagga tctcaataat ccggtagagt gggttaaagg gattggcaag atggcggcta
     2821 agtcggtagg caaagcagga attgagtcca caccttggtt gcgtgatagc gaagccatga
     2881 gcgcctattt tatgacggga gaacccgtac gccttgaagc tgcaaagcga ttgtttaaga
     2941 agttttatac ggaaaatatg aagggagatg ttcccatcaa aaaaatttct gtttcattat
     3001 acggttttga ccttggagct acactggcac gtaaattttt ggataatttt cttaaggaag
     3061 tttgcctgaa aaataaagaa aaaaaacacc aatataaaaa cgcgttggta gatattgctt
     3121 ttattggtct ttttgactgt tctcggcatt caccgactag tagtaataat gggttggact
     3181 atttttttat gtggttgggt ttaccaggga aaatgattgg ttcagtatta ggtgaaaaag
     3241 ctattgatca ggactcacca ctgccggacg tagtaaataa cgcacttcac cttgtagcag
     3301 ctcatgaacg gcgtccatgg cgtggtctgt atctgttggg gaaaaaaacg gatacagcga
     3361 ataaaaaata cagggaagaa ctgcttcccg gttgtagtga agatattggt ggtggcttga
     3421 aaccggatga acagaaaccc agtgcagaac tgtgcagggt tgcactatac aatatgtatc
     3481 atgcagcatg tcaagctggt gtgccatttc ctgattttga cactttggat caatatgata
     3541 cgaaagttgc cagctatttt attatgaatg atgctgttca gggaggatct gtaaattact
     3601 gggttagtcg ttatcaaaga gaggaagtga aggataaagt atttagtgag gcaatgcagg
     3661 agaagtatct agataactat tttctctggt tgggtgagca atattatatg tatcagtatg
     3721 aacgggaacg tctggataag gagttgacat cggctcatcg tgggaaaata agtgactatg
     3781 ggccacttgc aggtacggga attaatccaa atactaacgc tgatgatata aaatcgcaga
     3841 tttcagaact tgactcacta tggggatggt tgaatgatgt aaaaagtgta gctattggat
     3901 tacgaaatga ctttatagtc aacccgagga aaaatgatag ccgaataaaa cttaaacctc
     3961 aggtttataa tgctgctgtc aatcgggctg tgaaattcct tgagttttct cgcgcagttt
     4021 ataatgataa aaatctgcca tcatcatcat ctttgacggc aaatacacta tattcctttt
     4081 ttgtgcatga tatacaaaag
//
LOCUS       DACADF010000255         4089 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.258, whole genome shotgun sequence.
ACCESSION   DACADF010000255 DACADF010000000
VERSION     DACADF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.258"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(41..1597)
                     /locus_tag="ISB30_004675"
     CDS             complement(41..1597)
                     /locus_tag="ISB30_004675"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP1992221.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTSV
                     NKIYDPAAGSGSLLLQAKKQFDNHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTKVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSCYVEAKDNREIINIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGCEVQK"
     gene            1780..2001
                     /locus_tag="ISB30_004676"
     CDS             1780..2001
                     /locus_tag="ISB30_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP1992222.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            2001..2381
                     /locus_tag="ISB30_004677"
     CDS             2001..2381
                     /locus_tag="ISB30_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP1992223.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            2386..2565
                     /locus_tag="ISB30_004678"
     CDS             2386..2565
                     /locus_tag="ISB30_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HAP1992224.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLAGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            2593..2952
                     /locus_tag="ISB30_004679"
     CDS             2593..2952
                     /locus_tag="ISB30_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pdcB"
                     /protein_id="HAP1992225.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDSLVAWLRRKEGQVRLGHVVLA
                     GQQAYHESTGNGMGNPW"
     gene            2876..3289
                     /locus_tag="ISB30_004680"
                     /pseudo
     CDS             2876..3289
                     /locus_tag="ISB30_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(3239..3556)
                     /locus_tag="ISB30_004681"
     CDS             complement(3239..3556)
                     /locus_tag="ISB30_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I deoxyribonuclease HsdR"
                     /protein_id="HAP1992226.1"
                     /translation="MPERENYQQVTLQARLVSALETLNPNVPSDTLIEVAKIVATPQT
                     PVLIKNNKLFHQYLIEGVPVEYSVMEQEQWVTKHTHVRLIDFVLWSTKTGHRVRVFPV
                     SVF"
     gene            complement(3784..>4089)
                     /locus_tag="ISB30_004682"
     CDS             complement(3784..>4089)
                     /locus_tag="ISB30_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP1992227.1"
                     /translation="APQREELAEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKA
                     SIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
BASE COUNT         1033 a          942 c         1014 g         1100 t
ORIGIN      
        1 tcaaccccat ccagcaattt ttccagataa ctcaactcgc tcatttctgc acctcgcagc
       61 cttcaatttc agccacaatc gcatcaatat ctttacgcaa ctggtcgatt ttgctgaccg
      121 tagttttaag ctctgcattt agctcagcaa tattgataat ttcgcggtta tctttcgctt
      181 ctacatagca gctcaccgac aggttatagt cattagcgac aacggtctca aacgcgacag
      241 atttcgccag atgagcaaca tcttccttgc tggcaaatac ctgcataatc tgttcgatat
      301 gggcatcggt caggatattg ttgttggtct cttttttgaa tagttcgctg gcatcaataa
      361 actgaacttt ggtatccgtt ttatgtttag acaacaccag aatattgacg gcaatggtgg
      421 tgccaaagaa caggttcggt gcgagtgaaa tcacggtttc gacatagtta ttgtcaacca
      481 gatactgacg gattttctgc tccgcgccgc cacggtaaaa aatgcccggg aagcagacaa
      541 tcgcagcacg acctttggca gaaagatagt tcagcgcatg taatacaaac gcaaagtcag
      601 ctttggattt gggggccaga acgccagccg gggcaaaacg ttcatcgtta atcagcgtcg
      661 ggtcatcgct gccaatccat ttcaccgaat acggcgggtt agaaacgatg gcatcaaacg
      721 gtttttcatc tctgaagtgc ggttcagtca gcgtattgcc cagcttgata tcaaacttgt
      781 cgtagttgat gttgtgcaaa aacatgttca tacgcgccag gttataggtc gtatggttga
      841 tttcctgacc aaaaaaacct tcttcgatga tatggttatc aaactgtttt ttcgcctgca
      901 acaacagtga gccggaaccc gctgccgggt cgtagatttt gttaacgctg gtctgcccgt
      961 gcatagccag ttgtgcaatc agcttggaga cgtgctgcgg tgtaaagaac tcaccgcctg
     1021 acttaccggc atttgccgca tagttagaaa tcaggaactc ataggcatca ccgaacaggt
     1081 caatctgatg ttcgttgaag tcaccaagtt ttaacccttc aacccctttc agaaccgcag
     1141 ccaggcgggc atttttatct ttaacggtgt tacccaggcg gttactggtg gtatcgaaat
     1201 cagcaaacaa acctttgatg tcagcttctg aaggataacc gtaagcagaa ctttcgatag
     1261 caacgaagat gctgtttaaa tctgcattca atctgtcatt ggtatttgct ttcgcagcta
     1321 cgttgcagaa aagctggctt gggtagatga agtagccttt ggttttgatg gcatcgtctt
     1381 taatgtcatc agtaattacg ctgtcatcca gtttcgcata acagatactg tcatcaccgg
     1441 cttcaatata actggaaaaa ttttcgctga taaaacggta gaaaagtgcg cccagaacgt
     1501 attgcttaaa atcccatcca tcgaccgaac ccctgacatc gttagcaatt tgccagattt
     1561 gacgatgaag ctctgcacgt tgttgaatac ttgtcatttt catccactta tttcaggctt
     1621 atgtaattgg cggtgattct acagcaactt ggatgcttta gcagttcgga cattaggcta
     1681 cgaatgacct gcctagaggt ttgttaagcc gcaaagtgct ggtgctttat gcctgtgaag
     1741 tttataattg tgtacacata acgagtacac gaggtgttta tgcaatccat taacttccgt
     1801 accgcgcgcg gcaacctttc tgaagtgctc aacaatgttg aggccgggga agaggttgaa
     1861 atcacccgca gaggccgtga gccagcagta attgtcagca aggctacttt cgaagcctac
     1921 aaaaaagcgg cgctggatgc tgaatttgca tccctgtttg acaccctgga ctccaccaac
     1981 aaggaactgg ttaaccgata atgaggcata tatcaccgga agaacttatt gcgcttcatg
     2041 atgcgaatat aaaccgctac ggcggcctgc cgggaatgtc tgatccgggc agggcagagg
     2101 ccattatcgg gagagttcag gccagagttg cctacgaaga gatcaccgac cttttcgaag
     2161 tctccgccac ctacctggtg gctacagcga gagggcatat attcaatgat gccaataagc
     2221 gtaccgcgct aaacagtgcg ctgttatttc tacgccgtaa cggggtgcag gtatttgatt
     2281 cacctgaact ggcagacctt accgtagggg ctgcgaccgg agagatatct gtatcttctg
     2341 tcgccgacac gttacgtaga ttgtatggtt ctgcggagta gattaatggc acgcaaatac
     2401 aacaaattgt cccgtgaagc gttaaagatg cttcttgatg gcgtgagtcg ccgcgaggta
     2461 aagcaatacc tggctggtaa gcaaattggt gccaggaccg ctattgctgt gttatgccgt
     2521 caggaaatgg ttgtgcttaa acagagaatg ccgggcagca gataaagccc aatcagtgat
     2581 gaaaggtgtg atgtgaaagc cgtaattact ccctttgtac aaaaagagct tggcgtcgcc
     2641 acattcaaag tggatcagga agtcagaaag ctggtggagg ctggccgtaa atttattatg
     2701 gagccggtac cgcgtgagtt aatcgagcac atggacgacg gcctcgttgt ttccgagcaa
     2761 actatggcaa caaatgaggc gttgcagccg ttttttaaca gcgatgaact gtttcgccgt
     2821 attggtggaa ttgactcgct ggtagcgtgg ttgcgcagga aagaggggca ggtcaggctg
     2881 ggccatgtcg ttctcgccgg acagcaggct taccatgaaa gcactggaaa tggcatggga
     2941 aacccgtggt aagcccgtcg gggtgatgtt ccacagcgat caaggcagtc attatacgag
     3001 caggcagttc cggcagttac tgtggcgata ccggatcagg cagagtatga gtcggcgtgg
     3061 aaactgctgg gataacagcc caatggagcg cttcttcagg agtctgaaga acgaatgggt
     3121 gccagcgacg ggctatgtaa gtttcagcga tgcagctcac gccataacgg actatatcgt
     3181 tggatattac agcgcactaa gaccgcacga atataatggt gggttaccac caaacgaatc
     3241 agaaaaccga tactggaaaa actctaacgc ggtggccagt tttagttgac cacaacacaa
     3301 aatcaattaa tcgaacatga gtatgcttgg tcacccattg ttcttgctcc attaccgagt
     3361 attcaacagg cacaccttcg attaaatatt ggtgaaagag tttgttgttt ttaatcagta
     3421 caggcgtttg cggtgtggca acgattttcg ctacttcgat taaggtgtca ctgggcacat
     3481 ttgggtttaa ggtttccaag gcagagacta aacgcgcctg tagcgtgact tgctgatagt
     3541 tttcacgttc aggcatatca ccatctggtg cgatgtcata gccattttgg tattggtaac
     3601 ctagctcttt aaaccaatcg aggcattgtt gttctaggga aggtgcgaat aagcggggaa
     3661 attcttctcg gctgactcag tcatttcatt tcttcatgtt tgagccgatt ttttctcccg
     3721 taaatgcctt gaatcagcct atttagaccg tttcttcgcc atttaaggcg ttatccccag
     3781 tttttagtga gatctctccc actgacgtat catttggtcc gcccgaaaca ggttggccag
     3841 cgtgaataac atcgccagtt ggttatcgtt tttcagcaac cccttgtatc tggctttcac
     3901 gaagccgaac tgtcgcttga tgatgcgaaa tgggtgctcc accttggccc ggatgctggc
     3961 tttcatgtat tcgatgttga tggccgtttt gttcttgcgt ggatgctgtt tcaaggttct
     4021 taccttgccg gggcgctcgg cgatcagcca gtccacatcc acctcggcca gctcctcgcg
     4081 ctgtggcgc
//
LOCUS       DACADF010000256         4088 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.259, whole genome shotgun sequence.
ACCESSION   DACADF010000256 DACADF010000000
VERSION     DACADF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.259"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(98..889)
                     /locus_tag="ISB30_004683"
     CDS             complement(98..889)
                     /locus_tag="ISB30_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HAP1992228.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNITAEIGYGHN
                     FPSKGTIPDRRNFFENPRIYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGHELEIIPLS
                     DISRFSEDVKLKVLYKTSPLAGAIMELDSVSYLKSSRHTHAVEHKHPVHKAELTFVTN
                     EDGIVTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
     gene            complement(896..2866)
                     /locus_tag="ISB30_004684"
     CDS             complement(896..2866)
                     /locus_tag="ISB30_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HAP1992229.1"
                     /translation="MYMNVIRNVICTLIILPIGLQAETSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVVTNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGIRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGLSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVGITDSNKAGASLRLYRNNTTEPGNPTQTHGDSGLRDRKTVQNDV
                     QFWYQYAPVDNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTGNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            3103..3587
                     /locus_tag="ISB30_004685"
                     /pseudo
     CDS             3103..3587
                     /locus_tag="ISB30_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1046 a          964 c          930 g         1148 t
ORIGIN      
        1 tgctcccctg gcgcgtagca ctgccgactg aataacacat ccccgtcaat acggttcttg
       61 ctgcacgctt acaatttcag ggcagggaat agtattgtta atttcgggag aaacttaaag
      121 ttgccacatc gacagtttca tcacacaggc ttttgtcctg aaaacttttc ttattttgga
      181 ctttcgccag ccactgtcca atatgaagag aaggtactgt gacgatacca tcctcattag
      241 ttacaaaggt gagttctgct ttatgaacag gatgtttgtg ctcaactgca tgagtatgac
      301 gggatgattt cagataactg accgagtcaa gctccatgat agctccggcg agcggggacg
      361 ttttatacag gactttcagc ttcacatctt cactgaaacg acttatatcg gataacggaa
      421 tgatttcaag ctcatgcccc gttggagact gatagagttg tgctggcatc tgctgttcac
      481 caggaataaa agatttcgca tatttagtga caaactcaca gtaagccaca tcatttctgc
      541 cctcccggct gaccggtttc catcctgaag aggttctcga ccagtatccc ggtttcatat
      601 atgttgacaa aacgtagcca ttatctttgc ttgcagactc agttttatag acataatccg
      661 tggacgctgg cttcagtgtt attgtctctt tcccgttata aatccgggga ttttcaaaga
      721 aattccttct gtcaggaatt gtgccctttg aggggaaatt atgaccataa ccaatctctg
      781 cggtaatgtt gttctctgcc tgtttacctg taatccagac atcgtgggcc aggacatgcc
      841 ccgatgctgt cagacaaaga gctaagatat aacctgtatt tttcatcatg atttgttacc
      901 actgataacg ggtataaaga ctgacagttc tgcctgaaag cggcactcct ttgccattca
      961 tggcaagctt gttgaaggca ttatccagag tcactgatgc ctcaaagcct ttaacactta
     1021 caggcttata agacagtgaa aaggaatgta cggcatagcc agcctgatgg ggatcgcctt
     1081 ttttcacatg cgtggctgct gccgaaagtt cagcacgcca tccggcagaa actcctccat
     1141 gaccaacagg tatacttatg tcactgatga gtgtgtcagg attgccggag cccagccatt
     1201 ccctgctgct ggcatccata ctttccgtgc ggttataaga aagcatccag tcaaaataat
     1261 cagactggta atttccctgc atatcccatc cccagatacg ggcccgggat acattataag
     1321 aataagaacg catccgtttc atatccacgc gcgtggcaat atagtttctg gcatccgttc
     1381 cgaaatatcc gcctttcaac tgcagccgat cattgcctgt aagcaggccg tccagttgaa
     1441 tacctgcgcc aatctcacgg gtaatgttat tttctggttt cagattaagg ttaggtaccc
     1501 agtaattggg tttacccttg cggtaaaaat gtacatcatc cctgtacatt tctgccattg
     1561 tgggggcacg gaatgcagag gatatggagc cgtacatcat cagccagtct gtcggtgaga
     1621 ctgaaatcgc cgcccttggg gacaggcgtt cggctttcag ttctccggca cggggattga
     1681 agctcttgta ccggtcatag cgggaaccga cgataatgtt aaccgggtag cttttcattg
     1741 tcatttcatc ctggaaatac aacgatgtaa agtcaatgtt tccttccgga tatagtgtgg
     1801 cagagccttc tggcttctgc tgctgacggt aatattcagc gccataactt aactgatggg
     1861 ctcccggaaa aatcagagta tgactcctgt tgacaacatt aacaccggag gttctgttgt
     1921 ttctccattc tgccgttttg ttgtgaccgt ttgtcttgat agtgatatca ctgagatata
     1981 aagttgactt tacattgatg aggctgttat ccacaggagc gtactggtac cagaactgta
     2041 cgtcattttg tactgttttt ctgtcacgca ggccgctgtc accatgtgtc tgagtgggat
     2101 tgcccggttc agtggtgtta ttccggtaga gacgcaagct ggcacctgct ttgttactgt
     2161 ctgttatacc gacagagcct ttcgcaaaca gggctgcagg tttttccttg ttaggtgcgc
     2221 gctcaccatc actttgatag atattacctc ttttacgcat aatgacagaa aggagcgcat
     2281 ctgtttttcc ggttttaccg aaccaggtga gccccgagcc tgtgctgtgg tcaccactgg
     2341 cgatatttcc ccacagactt aaaccgtttg tctctccggg gggaaggaaa tctgctgcat
     2401 cggcagttct gaagtccaca acacctccca gcccgccact gccgtacaga ctggagtttg
     2461 gtccgcggac aacttcgata cgtttgacga gtgccggatc cagataagtg ccactgcttt
     2521 ttgccatgtc actgagttga cggatgccgt caacaagaac aagtaccccg cttttgtcat
     2581 agccgcgtag attaaaggtc tgcccattgg tccgtcctgc accagtctgg ctgagaccgg
     2641 caacaccttt cagcatgccg gctgatgtga ccgcattctg actccatggc gtgtcattcg
     2701 tgacgacact gaccattgac ggcgtttcaa ataccgtgtt ctgatttcct gtcgcgacaa
     2761 cggtgattgt atctttagcc atagaagaat gactggtctc tgcctgtaac cctatcggga
     2821 gtatgattaa tgtacaaatg acgtttctga ttacgttcat atacatgcga aaatatatgt
     2881 taatattgat atgttataac atatcatttt attaatgcaa ccctgatcac gcagcgttca
     2941 gtgataacca gtgaatagcg actctaatac ccggtcgctc tgcaatgtat cagcgcctgc
     3001 gaccgatgct atggatattg atttttctac cgataaagaa ccagcaatac ggaatttaga
     3061 ctggccccct gaatctccag acaaccaata tcacttaaat aagtgatagt cttaatacta
     3121 gtttttagac tagtcattgg agaacagatg attgatgtct taggaccgga gaaacgcaga
     3181 cggcgtacta cacaggaaaa gatcgctatt gttcagcaga gctttgaacc gggaatgacg
     3241 gtctcccttg ttgcccggca acacggtgtg gcagccagcc agttatttct ctggcgcaag
     3301 caataccagg agggaagtct tactgctgtg gccgcaggag aacaggtcgt tcctgcctct
     3361 gaacttgctg ctgccatgaa gcagattaaa gaactccagc gcctgctcgg caaaaaaacg
     3421 atggaaaatg aactccttaa agaagccgtt gaatatgggc gtgcaaaaaa gtggatagcg
     3481 cacgcgccct tattgcccgg ggatggggag taagcttagt cagccgttgt ctccgggtgt
     3541 cgcgtgcgca gttgcacgtc attctcagac gaaccgatga ctggaaatat aggggcaaat
     3601 ccacatcctt acttttccgt aaggatttta gcgggtcaaa acagcgttcc atgccggact
     3661 gatgatctac atacttagag tcacatacca aagtaaaact atggatgtat gcaagcctgc
     3721 atcgtcagga ggttaaattt cagacctgcc agttaccgcg taaatggctt gatatatgac
     3781 taatttattt gcacaggatt tttacgacaa cgctctgaac cagcagttga cgggatatat
     3841 agttgagctc attacagata aagcttttct gtatctctgg cggtggtcat agaccagtaa
     3901 tcgttattgg ttgttctata ttatcccgta tgacggtaca acgtttttaa caactggcgc
     3961 tgtcacaaca taagccaccc caaaatatta tatgagtaac aatagtctat ataggagcgt
     4021 aaattaccta ctatcaaata cttactccaa tagcatttct gtctattcta tttcaattct
     4081 ttaataga
//
LOCUS       DACADF010000257         4073 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.260, whole genome shotgun sequence.
ACCESSION   DACADF010000257 DACADF010000000
VERSION     DACADF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.260"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            75..776
                     /locus_tag="ISB30_004686"
     CDS             75..776
                     /locus_tag="ISB30_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP1992230.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPD
                     EQKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRA
                     QYFKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTG
                     DRTIAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1034..3181)
                     /locus_tag="ISB30_004687"
     CDS             complement(1034..3181)
                     /locus_tag="ISB30_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HAP1992231.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEAL
                     PKDSNSAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGL
                     AALRAQLLKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGN
                     GEMTCYLLLHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQR
                     VYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQG
                     RLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVI
                     SALTDKGLRYAGYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLF
                     RSLGFNRTWEEWQREEDARTHTTERRHDRGWSQ"
     gene            3360..3614
                     /locus_tag="ISB30_004688"
     CDS             3360..3614
                     /locus_tag="ISB30_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAP1992232.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
BASE COUNT          963 a         1030 c         1066 g         1014 t
ORIGIN      
        1 aaaaaaaaaa cttccaccca tatgataagt ggcgtacagt aaaaaaacgc caccaatagc
       61 aacgagcaag cgaaatgtca gatctgatag cttacaaatc aaacgccctt atcgaggcaa
      121 gctacaagct cactttgcag gaacagcgct tcctgctggt ctgtatcagc cgcctaaaat
      181 ccggagaaga ttccccttcg ccggatgaac aaaaaaccat gaccatcacg gctgcagagt
      241 ttcatgatgc ctttcctgac atgggacggc agcacgctga agctaggctc aaggaagcga
      301 tagatcgcct ctgggacagg tcagtaatca tcaagaacga ggaaaaacga gaagagttcc
      361 gctgggtgca gtaccgggcg cagtatttca aaggtgaggc gaaagtagat atcaccttct
      421 ccgatgccat catgccgtat ctgacacagc tcaaggggca gtttacgcgg gtagtcgtga
      481 agaacgtctc cagcctttcc agcacctaca gcatccgcat ttacgagctg ctccagcagt
      541 tccgcagcac cggtgacaga acgatagcta tcgatgattt ccgaaccatg cttgccgttg
      601 aagataaata tcaacaattc aaagacttga ataagatgat aatcaagcca gccatcgccg
      661 aactgaacga aaaaagcgat ctggttgtaa ctgttgagac gatcaaaaag gggcgcacgg
      721 ttgtagccct gcattttcgc ttcaaggaag acaaacagat caagatgacg atctaaaatc
      781 gattttaagg cacctaaaac gctccctgct gcgttttttt tgtgttagta gtgcgaaggt
      841 actattgaag tgaaaaattg cgtcaaaaac gctctgatgc agcgaaaaaa gaccccccaa
      901 tttttggggg cggaaaaaca aaactatgct tgcgttatgt gagtgtgctt cttgcattca
      961 tccctgtcga tgaacatcga agaacatcga cgaacataac acgatgttaa acaaaacaaa
     1021 atattgttca cattcactgt gaccaaccgc gatcatgtct cctctcggtg gtgtgtgttc
     1081 gtgcatcttc ctcgcgctgc cattcctccc aggtacgatt aaaacccagc gagcggaaca
     1141 gatgatccgg ctgtttttct ggtgtgttga cggcagcaaa aagcggtgcc tcacgggttt
     1201 tcagatgaaa ctgcatcagt tctggtgcag gtgatatgtg gctgtctgca tcgtgattta
     1261 cggcaacacc ataccctgca taccgtaacc ccttatcggt cagcgcactg atgaccagat
     1321 gcctgttatc ggctgaggga acggcaaacg cgggatacag cgtaccatta agccggataa
     1381 caccgcccag agagtcatcc acgcccatga ccagcaaaag ctgaagctgg cgggtttccc
     1441 ggtcgacaga actcatgacc ggcatcagcg tcggtggcag acgtccctgc ataaaatcca
     1501 ccttccttgc cgctggtgat ggcatggctg gctggcggtc ttctccaaga cagaaagact
     1561 ccggccatgt cttcggctgg cggagattgc gggtatgttg cggccatgtc tggcaaacct
     1621 gcgtcagcag ttgctcaaag gttgcagagg aataaacacg ttgcagtggc ggacgttggc
     1681 cgatttcgct tcggtcacag atataaccac tcctgtcacg cattaaatca acactctcaa
     1741 agattctggt gcggtatttc tccagctcgt tcccccagag cgtatattct ccgttccggt
     1801 tgtgaagcag aaggtaacag gtcatttccc cgttaccagg catgggagcc tcgccatacc
     1861 ccgtaacgac tgctctcagc acccctgaat ccataacggc attgtcattt ttcctgagct
     1921 gctccgcgcg actacgttca gtatcttcct gttccttccg gcgcaattcc tcttcacgtt
     1981 taagcaactg ggctctgaga gctgcaaggc cggattcccc ccactgctgc cactgttttt
     2041 caaatgccat ctgactcgcg ggaatgtcct gtttctccac cttccggtaa tgctgtcgta
     2101 atttttccat ttgctggctc agagactgga tttttccctg ctcactacgg tattccggtg
     2161 ccgcccattg catcagggga atacgggaaa aaccggacga aaacggcgat gattttttca
     2221 ggctggcagc atggctcatc gcttcctttc gttcggtgtt cagctcttcc cagcgtctgc
     2281 gcgtcgcctg catatctgcc atcatcgaca tggcattact ggtgagtttc catgccgagt
     2341 tactgtcctt cggcaacgct tcgataatgt cacgtagtgt gtacgaagtt gcgttgtcag
     2401 gaagcccgat attcagaagg tcagccacac ggcgggcagg ctctgtcagc cctccgttcc
     2461 tgagttcggt gcgggaaatc tgtatttcca ggttaagcct gttaatcaga cgattctgtt
     2521 cgccgcgttc tgtttcgata ccgcgtgttt ccatcgcaca ggcagacttt ccgaggtgaa
     2581 taccaggcgt tttctcaagc ccctgtcgct cgtatgaacg atggtctatc tcttcctgat
     2641 aaccggcgtt cgccagtgcc ctgtttgcat ggtcagccca tgatgcccgc cacgtctcgg
     2701 catgtgtccg gtcgttccag tccctgacct ttccgctgaa cccagcaggt ccgatagccc
     2761 tcgtggtcag catgatgtgc gcatggggat ttgttttatc catgtgatgg aacgccacat
     2821 cagcaatcat gccctgactg acaaagtttt ctctgacaaa ggcgagaacg gtttcccgtg
     2881 cggcatcctg cggcagttcg tgggggatgg cgagttcaat ttcccgcgca agttgtgagt
     2941 tcttccggcg ttcggccttc tcaacggcat tccagagttc ggcacggttc gtacaccagg
     3001 caggggcggt ggcgggggtc agaatcacag cctcagccac accgcttttg cgggtgtagt
     3061 cgtgagtcag tccggtacgc tcatcaagca tcagtgaacc ggaacgatag gcagcggaag
     3121 ctacggcgct gtagccgctg cttctggaga taattttcat gctgagatga taaatcgcca
     3181 tttctgccgt aatgcctttt tgtagttttc tttcagcccc gcagggcgca cacaatcgaa
     3241 acggagtgga gattgtataa gtgcgcactt cgtgatttgc cgtgcattat gccagctcag
     3301 tttttaaact accagtgacg cgctaaaatg cgatttttca tcgcgcacgg agttacatca
     3361 tgagcagaac gctcgaacag aagattgctg atgctgaggc gagattacag cgcctgaagg
     3421 cgaagagccg gagcctggac acagcgcaga aggttgttgt gggtgctgca ttgctggcaa
     3481 aggtcagaaa gccggaggag gtgcagttgc gtgcctggtt actccagttc ctgaaggcag
     3541 aggtgacacg acaggctgat gtgacgcgca tactgccgct gattaacgag ctggaggcgc
     3601 tgccagaaca gtgattgtat cgtccgctcg ccgcagcccc agcgaccgag cgttagcgag
     3661 cgagtgggcg aggaagcgga agtgaatcag tcgccaggtt gagcacatac gcacggtttt
     3721 tcggcaggtg atatgcgctg aacccgcatg aatactgggt ttccacaggg ttggaggggg
     3781 tgtattcgaa acttgcaatt ctgctttatt gcaacgcatt gtttttattg gttttttgtt
     3841 ttcagcaccc caagttatcc acaggctcgc cgttaagttt ttaagatctt atttttagat
     3901 cttttttttt aaaacctttt taggtcttgt aacaccatga ttttaaagat gttctggcaa
     3961 gataagtgga gcttttgggg gtgaaaggtg gagcttttgg gggtgaaagg tggagctttt
     4021 gggggtgaaa ggtggagctt ttgggggtgt aatccacaga aaaagggggt tgc
//
LOCUS       DACADF010000258         3882 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.261, whole genome shotgun sequence.
ACCESSION   DACADF010000258 DACADF010000000
VERSION     DACADF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.261"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            5..589
                     /locus_tag="ISB30_004689"
     CDS             5..589
                     /locus_tag="ISB30_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992233.1"
                     /translation="MAICMAMVLLFLYINKDNIKVSYSLKINQTTPGILVSCDSNNNF
                     ACQTTMTEDVIQRITTFFQTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWY
                     TSEYHNGRQVLDEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPA
                     AICLALGFFLSIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(832..1527)
                     /locus_tag="ISB30_004690"
     CDS             complement(832..1527)
                     /locus_tag="ISB30_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAP1992234.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAIVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1520..2947)
                     /locus_tag="ISB30_004691"
     CDS             complement(1520..2947)
                     /locus_tag="ISB30_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAP1992235.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQENKNG"
     gene            complement(2958..3677)
                     /locus_tag="ISB30_004692"
     CDS             complement(2958..3677)
                     /locus_tag="ISB30_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAP1992236.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1015 a         1022 c          818 g         1027 t
ORIGIN      
        1 tattatagct atctgtatgg caatggtgct gttgtttctg tatatcaata aagataatat
       61 aaaagtgagt tacagcctaa aaataaatca gacaacacca ggcatacttg ttagctgtga
      121 tagcaataat aactttgcct gtcagaccac aatgactgaa gatgttattc agcgaattac
      181 tacatttttt cagaccagcc cagatgtcaa gaacagagaa ataaagctgg aatggtcagg
      241 agataagaga gatttaccaa ctgctgaagc ggaaatatct cgcgtgcagg cgtctatcat
      301 taaatggtat acgtcagaat accataatgg caggcaagtt ctcgatgaga tacaaacgcc
      361 ttcagcaatt aacagtgagc tttatacaaa aatgatatat ctgaccagga actggtcact
      421 gtatccgaac ggtgatggct gtgtcactat aagctcacca gaaataaaaa ataaataccc
      481 tgctgccatt tgcctggctc tgggattttt tctaagtatt gtgatttctg taatgttttg
      541 ccttgtcaaa aaaatggtag atgaatacca acaaaactct gggcagtaat aaacagcatt
      601 ctgacagaga gtgcctgtat aaaactatag tgacagtgac cgggaaggga gcccgtcttc
      661 aggaatggat tcctgtcttt ttaactgtaa atatcctggc attattaatg tcaggatatt
      721 tacagaagag cagcataacc atgacggaac ggggttaagt caggttgtcc ttaacgctat
      781 tcacaacctc gtataccgcc ttcaggttta tgtccagact acatatctct ctcagcaatc
      841 ctcaataatc agatacaccg ctttcaccgg accatgaacc ccgacgactt tgataagctc
      901 aatgtccgcc gttgaactgg gaccactgat gatgttaata catgacggca ttcgttcacc
      961 ggcctgcgct ttctgatgca atttttctgc aagttgcgct acacgcggca ggattgtgct
     1021 tttacgcagg ataaaaagag aagattccgg gagcaggctc aatgaacgcc cgcgcccggc
     1081 ggcggaaaaa agaaccacgc ctcccgattc ggttaaacca tattcagcat atacgacgcc
     1141 cactatagcc tgctctgctt gtgatatgtt ctcaatacct ttcgccggat cccaaacaac
     1201 ggcattgcat tcctgctgca aacgttcgct aatccccaat tcctccagcc tcgtgtcacc
     1261 gctaatcacg accgactgat ctcccagctc tttacacaga cgtattgcag cttctgccgc
     1321 cttcgcctcg ctggtcagtt cacagcgcgt caacataaca tcgctggcaa actggataaa
     1381 cgcgtcacag cgctgttgtt gggtaagttg ggtaagccgc tcgttagcat agttgttaag
     1441 cggcgcatct tctgcttgcg gttcaagtcg cagcgggcga cccagtgcct gagcaacgtt
     1501 attcaaaaat tcgctccgat tatccattct tattctcctg cgcctgatgt ttcttaaacc
     1561 aactacggaa actctctccg tcagcttcag gaagatcgcg tgcttccatc cagtcgctaa
     1621 tcgcgccaaa tttgagtggt gttttgccgc cattgataaa ccagcttgcc gcatgagcac
     1681 cagccatcat cccaactttc cacaatcctg gatgactatt ggcataagcg aacattttta
     1741 tcgcccgttg ctctgctttt gcggtgatcc ctttttcagc catcacccga cgatgacgca
     1801 aaatcagttt tgacagcgga atacgcaccg gacacacgct gtcacaagcg gtgcataaag
     1861 agcaggcgta gggtaaatct ttaaaatctt tatagccgcc aagtaacggg gaaatcaccg
     1921 caccaattgg ccctggataa atagagccat atccatgacc gccaatatgg cgatatgccg
     1981 gacaagtatt catacaagcc ccgcaacgaa tacagcgcag cacatcccga aattcagagg
     2041 ccagcacctc agaacgcccg ttatcgacaa taaccagatg aaactcttca ggaccatcaa
     2101 cgtgccccgc ttcgcgtggt cctgtcagcc aggtgttgta tcccgtcaaa cgtgcaccaa
     2161 cggcactgcg cgccagcatg gtgatcaata catctacctc tgcaaacgtg ggagcaatac
     2221 gctccattcc catcactgca atatgcgttt taggcagcgt ggtacacatt cgcgcattac
     2281 cttcattggt caccaggcat accgaaccgg tctctgccac cgcgaaatta cagccggtaa
     2341 tacctatttc agcactgagg aaatcttcgc ggattttttg ccgaatgaat aaggtcatcg
     2401 cttcaggcgt ttccggcccc tcatagccca gacgttcgtg tagcactcga cggatctgat
     2461 ggcgatcttt atgaattgcc gggaccacaa catgagaggg cggatcttga tccagctgga
     2521 gaatatattc acccagatcg gtttcaatca cctgaatccc ggcatcctgc aacacatgat
     2581 tgacaccaat ctcttcggtc accatcgatt tagatttcac taccttccgg gcatttttgc
     2641 gttgggcaac ctgtaaaatg tagcgggtgg cgtcttcttt ggtttttgca aaatagacgt
     2701 gaccgccgtt ttgtgtcact ttttctgaga gctggtacag ataagcgtcg agattactca
     2761 gaacatgatc acgtatctgg gcggcccgat cgcgccactc ctcccagtgc cccaattcat
     2821 cgaccatttt ttgccgattt gccccaatac gctgctgcgc gtttgccacc gctttgcgca
     2881 tgatcggatc ttcaatttgc tgacggatgc gtgtcttaaa atctgtatta ctggttttga
     2941 tcgacatctt tatatcctca gcggctcatc agcacttcag caatatgcat cactttgact
     3001 ttctgccctt cccgttgtaa tcgtccactg atgttcagca ggcaactcac gtcagcacca
     3061 attaaatact cagggcggac ttccatcagg tgcgcaacct tttctttcac catctcgccg
     3121 gatatttcgg ccattttgac cgagaacgtg ccgccaaatc cgcagcaggt atcctgttca
     3181 gcaaaggtca acagctccag tccacgcaca tttttcagca gcgtaagtgg ttcgtccttc
     3241 acccccagct tacgggccag gctacaagat gggtgataca ccgctctccc ttgcaaactg
     3301 gcacctacat cgactacccc taatgtatta acaataaaag aggtgagatc ctgcatacgc
     3361 gcggcaacct tttcggcacg tgatgcccat tcaggttcat ccgccagata cgtcgggtaa
     3421 ctttttacgg cataggtgca agagccagcc ggtgaaataa tgggatcgtc gttatcctcc
     3481 agtgcggcga tcagattttt catccctgga atcgcttctt tgatataacc actattgatc
     3541 gcaggctgac cgcagcatcc ctgtttctcc gggaaattta cgcgacagcc gagtttttcc
     3601 agtagcagca cggagtctcg tgccattctt gatttcaggg cgtcaccaat acaggtgaca
     3661 aagaaattga cattcacaac taatactcca ttacttcatg cccatttatg cccgatggtt
     3721 ccctccttcc gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa
     3781 cataccacct ctcatttgta tgacaaatga taatctttca tctaaacttc aaatgcaata
     3841 actaatttta tttctttcgg catcgctcac attttatagg ga
//
LOCUS       DACADF010000259         3877 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.262, whole genome shotgun sequence.
ACCESSION   DACADF010000259 DACADF010000000
VERSION     DACADF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.262"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..391
                     /locus_tag="ISB30_004693"
     CDS             <1..391
                     /locus_tag="ISB30_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tunicamycin resistance protein"
                     /protein_id="HAP1992237.1"
                     /translation="QDLPLWRGLTIAAVREIRRNYSQDIIIPMTLVHPDYLTEILDGV
                     RRIDDQLLHIFLTLNEDLLRHRIANQTMHPDPNRNAEIREWRLANVARCLAARERLPC
                     TTRVLDSGAHTSDELAAMVLDGIDGRT"
     gene            complement(873..1064)
                     /locus_tag="ISB30_004694"
     CDS             complement(873..1064)
                     /locus_tag="ISB30_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992238.1"
                     /translation="MAIALMGAGFSATDTSDAVNILYPITMTVQANKAWQASGLKKSF
                     FLPSHKKVRGGEKISYARL"
     gene            1088..1315
                     /locus_tag="ISB30_004695"
     CDS             1088..1315
                     /locus_tag="ISB30_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992239.1"
                     /translation="MVNSYSLSDDSGVMAAAAITHFLFGQAVFSYLNGWSVLIGPGTG
                     LDSTGCKYARDLMGLVAFTAFIVTFLFRGYS"
     gene            1366..2502
                     /locus_tag="ISB30_004696"
     CDS             1366..2502
                     /locus_tag="ISB30_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HAP1992240.1"
                     /translation="MSQLSQLRSPAAVQAAIDEFVQLGRTKFLARHGYGKSRDFLVRD
                     PKTGTDCDSKAIAGVAFGKQFPEQGPLTADSFSGGEATVVPALTRLGFRIIRIGEDWS
                     EEEVLATVEDYFDMLRAEAAGEPYNKSEHNQALRQLLNGRSKSSVELKHQNISAVLDA
                     LGLPYINGYKPRGNSQLLLRKSVHAYVLEHQQTVGALVDALEEVKLPGDKTYRAALVE
                     PPAREVLVRTPASLRQRLPRKFDYAARDEANRKLGRAGEQWVIGYEQQRLTELGHPEL
                     FQRLDWVSDTQGDGAGFDILSFEEDAHERFIEVKTTNGGVGSSFLVSHNELEFSKEAG
                     DQFHLYRVFQFRDGPRLFTLPGDLSQHVHLKPTDYRASFRSLVG"
     gene            complement(2469..2618)
                     /locus_tag="ISB30_004697"
     CDS             complement(2469..2618)
                     /locus_tag="ISB30_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992241.1"
                     /translation="MLFAPTLADKAILPTAWGGRYFFNRIGRTQPFGSTEPCLYPTKL
                     RKLAR"
     gene            2617..3877
                     /locus_tag="ISB30_004698"
                     /pseudo
     CDS             2617..3877
                     /locus_tag="ISB30_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971921.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISCfr1 family transposase"
BASE COUNT          798 a         1135 c         1125 g          819 t
ORIGIN      
        1 tcaggatctc cccttgtgga ggggacttac gatcgcggcg gtcagggaga ttcgaaggaa
       61 ttactcgcag gacatcatca tcccaatgac gctcgtgcac ccggactatc tgactgagat
      121 actcgacggg gtaaggcgga tcgacgatca gctgctgcac atctttctga cgctcaacga
      181 ggacctattg cgtcaccgga tcgcgaacca gaccatgcat cctgacccga atcgaaatgc
      241 ggagattcga gagtggcgat tagcgaatgt cgcccgatgc ttggccgcaa gggaacggct
      301 tccatgcaca acccgtgttc tcgatagtgg tgcacacacc agcgatgaac tcgcagcgat
      361 ggtgctcgac ggaatcgatg ggcgcacctg atcgccttcg acgcctgcgc aaagcgtagc
      421 gcgagggtgg cgggctcacg accaaacgcc cagaggtcga tcatcgcagg gatgtttggc
      481 tttgtggtgc ggacgacggg actcgaaccc gtactctcac agagaagcag attttcgtac
      541 cacctcgact ttcgccgccg tctgatgacg ttcgtggtct ggactgtccc ttcgccattg
      601 cccgaaggct ttaggcgccg cccgtccagt ctctacacct tcccccgaag gggcttggct
      661 cgggattggc ttagggtatt gcccgttagc gttccccgac tttgagcggt tctactccgc
      721 ggatttcccc gcgggcactc caattttaaa gtctgctgcg tctaccgatt tcgccacgtc
      781 cgcctttttt cgccgttcct agcgctcgtg cgatgcacct atgttgcacc tagcgccgaa
      841 tcgttcttcg tcatcctgaa aaaccacgtc tcctaaagcc ttgcatagct tatcttttct
      901 ccaccacgaa cttttttgtg ggatggtaga aaaaaagact ttttaagtcc gctggcttgc
      961 caggccttgt tagcttgtac ggtcatggtt atcgggtaaa gaatattgac ggcatcgctg
     1021 gtgtcggtgg ctgaaaagcc ggctcccatc agggcaatag ccatttcaga tgcaggcgta
     1081 cagggcaatg gtcaacagct acagcctgtc tgacgattcc ggcgtcatgg ctgcggcggc
     1141 tatcacgcat tttttgttcg gtcaggcggt gttttcgtac ctcaatggtt ggagcgtgtt
     1201 gatcggacct ggtacaggtt tggacagcac gggctgcaaa tacgcaaggg atttaatggg
     1261 cctggtggcg ttcacggctt ttatcgtgac gtttctgttc aggggctact cataatctcg
     1321 tggctcggcg gttcccggca caccatgaca gtaaggaagg accctgtgtc tcaactctcc
     1381 cagcttcgaa gccccgccgc cgtgcaggct gccatcgatg agttcgtgca actgggccgc
     1441 acgaaattcc tggcgcgcca cggctacggc aagtcccgcg acttcctggt acgtgatccg
     1501 aagaccggca ccgattgcga ttccaaggcc atcgccggtg tggccttcgg caagcaattt
     1561 cccgagcagg gcccgctcac tgctgacagc ttctccggtg gcgaggcgac cgtcgttccg
     1621 gcgctgacgc ggctcgggtt tcgcatcatt cgcatcggcg aagactggtc cgaagaagag
     1681 gtcctggcca cggtcgaaga ctatttcgac atgctgcgtg ccgaggcggc tggggagccg
     1741 tacaacaagt ccgagcacaa ccaggcactg cgccaactgc tgaacggtcg cagcaagtct
     1801 tcagtcgagc tcaagcacca gaacattagc gccgtactcg atgccctggg cctgccctat
     1861 atcaacggct acaagccacg cggcaacagc caactgctgc tgcgtaaatc cgtacacgcc
     1921 tacgttctgg aacatcagca gacggtcggc gctcttgtcg atgccctgga ggaggtaaaa
     1981 cttccgggtg acaaaaccta ccgagcggct ttggtagaac cacccgcccg tgaagtgctt
     2041 gtgcgtaccc cggcatctct acggcaacgc ctaccgcgaa agttcgatta tgccgctcgc
     2101 gatgaagcca accgcaagct gggccgggca ggggagcagt gggtgattgg ctacgaacag
     2161 caacgcctga ccgagctcgg ccacccagag ctttttcagc ggctggattg ggtgtccgac
     2221 acccagggag acggtgcggg gttcgacatc ctgtcgttcg aagaggacgc ccatgagcgc
     2281 ttcatcgagg tgaaaaccac caatggcggg gtaggctcgt ctttcttggt cagccacaac
     2341 gaactcgaat tctccaagga ggcgggcgat caattccatc tgtatcgcgt gttccagttt
     2401 cgggacggtc cgcgcctgtt cacgctaccc ggcgacctca gccaacatgt gcatctcaag
     2461 ccgacggact accgggcgag tttccggagt ttggtggggt aaaggcaggg ttctgttgag
     2521 ccgaatggct gtgtgcggcc gattctgttg aaaaagtagc ggcctcccca tgccgttggc
     2581 aaaattgctt tgtcagcgag cgtgggggcg aacagcatga tgggacagtt accgggagga
     2641 cagcagcgcc tgttctactc gttcaatctg gaagatcacg tcccggccca acatctcctg
     2701 cgcagcatcg accagtgctt ggatctcagt gatctacgtg cctacctggc agatttctat
     2761 agccccatcg ggcgtccctc gattgacccg gagttgatgg tgcgcatgct ggtcgtcggc
     2821 tacttgctat ggcattcgtt ccgagcggcg attgtgcgaa gaggtgcacc tgaacctggc
     2881 ctatcgctgg ttctgccggt tgggtctgga agacgaagtc cccaatcact cgaccttctc
     2941 gaagaatcgc catgggcgtt ttcgtgacag cgatctattc cgctggttat tcaatgaggt
     3001 gctgcggcgc tgcatggcag ccggcctagt caagggtgaa ggtttcgccg tcgacgccag
     3061 catcattaag gcggatgcca gccggcaacg tggggtggcg ggagatgagg tcgattggaa
     3121 cgatccaaag ctcagcagcc gcgcagtgcg cgagtacctc gaagcccttg atgaagaggc
     3181 gctggctgag gctcttccca agaaaatttc gctcactgat cctcagtccc gttggacagc
     3241 agcgccaggt ggcccggcct tttttgccta ctccacgaat tacctgatcg acactgagca
     3301 cggtgtgatc atggacgtgg aagctacccc ggcgcaccgt accgccgaag tcgattcgac
     3361 taggacgatg gtcgagcgtg tcgaggcgca gttcgatctc acaccggaac gccttatcgg
     3421 cgataccgct tatggcaccg ccccgatgct ggcctggatg gtcgaagaaa aggacatcga
     3481 accgcatgtg ccggtgtggg acaagaccga gcgcaaggac gacagcctct ccagtaacga
     3541 ctttcactgg agtcaggacg ccaatgaata tcgctgccca gccggcaaac cgctacgcag
     3601 tgaatggcgc gccttcaccc agcaaaggtc gcgggtaact aaggccaaaa ccgtcattta
     3661 ccgctccagc caaaccgact gcgccacctg cccgttgaaa gcgaaatgct gccccaacac
     3721 gccgaatcgg aagatcgtcc gcagcatcca tgaggctgcc cgcgacgtgg ctcgacgcat
     3781 cgccaagaca ccggagtacc tcgtctctcg ctgcgaacga aagaaggtgg agatgctttt
     3841 cgcccacctc aaacggatca tgaaactcga ccgttta
//
LOCUS       DACADF010000260         3834 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.263, whole genome shotgun sequence.
ACCESSION   DACADF010000260 DACADF010000000
VERSION     DACADF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.263"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            337..1827
                     /locus_tag="ISB30_004699"
     CDS             337..1827
                     /locus_tag="ISB30_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HAP1992242.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(1948..2520)
                     /locus_tag="ISB30_004700"
     CDS             complement(1948..2520)
                     /locus_tag="ISB30_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992243.1"
                     /translation="MQKLENRCELLLIQHQKWMTNVTRLIVAHGMGAPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2606..2866)
                     /locus_tag="ISB30_004701"
     CDS             complement(2606..2866)
                     /locus_tag="ISB30_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAP1992244.1"
                     /translation="MFSSPTPVEINNIRRNIFMNTPVSLMDDQMVDMSFITQLTGLTD
                     KWFYKLIKDGAFPAPIKLGRSSRWLKSEVEAWLQARITQSRP"
     gene            3700..3834
                     /locus_tag="ISB30_004702"
                     /pseudo
     CDS             3700..3834
                     /locus_tag="ISB30_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
BASE COUNT         1013 a          832 c          990 g          999 t
ORIGIN      
        1 tttattttat tcttcttcag gagggcagtt ttctgaaagc gttactccgg ggaaactgtt
       61 tatgtttatt cccggcagga aacccgtacc tgcaataccc tggatgatgc tttttgctgg
      121 tacgttgttg tgtgaaattt aatcaacgtg tgtgttccgg agagcgcccg tagacgcaca
      181 gattcattct gacattcttt agcgacaaaa taccgaatgg ttattattta atctgaaaat
      241 cgacagaaat ttttatcctg tattgattct gctgctattt ttgctattag tagcaatagt
      301 tgcggtactt tatgttctgt gtgttgtgag gtcattatgc catactttca gtatcctgat
      361 gagtttcccc tgagctcgct gcctccactc attcgtgatg ccgttattga agctcagcaa
      421 atcacgcagg ctcccctggg gcttgtggca gcatctgccc tgggcgctgt ttcacttgtc
      481 tgccagaatc tgattgatgt ctgcagactg aatacactac gggggccggt ttctcttttc
      541 ttactgacgc ttgctgagtc cggagaacga aaaaccgcag tggacaaatt attaatggag
      601 cccctgtacc agcaggagat gctgttatat tccagacaca aaaatgaact gacgacatgg
      661 aaaaataagg aagagctttt aaaggcgcag aaaaaagcat tgttgtcaaa actgaataaa
      721 gaactccgca aaggcgcgga tgagtcagaa acgctcagac aactggaagc gcttcagaaa
      781 aacagagggg aaaaacctgt aagatataaa ttcattttta atgatgccac gactgcggca
      841 attaagaatc agctttgtgg taaatggcgt tcagtgggta ttatgtcaga tgaagccggt
      901 ataatatttg atgggtatac cctgtctgag ctgcctttta ttaacaaaat gtgggatggc
      961 tccgtattgt cggtggacag aaagaatgaa cccgaacaga tgattgaaaa tgccagaatg
     1021 acattatccc tcatggtaca acccggtctt tttgaccgat atatggagcg aaaaggttct
     1081 gttgccaggg acagtggttt tcttgcccgt tgtctaatca gtaaacctgc caccacgcag
     1141 gggaaacgct ttattaacgg agctgtgacc ccgggaggat cattaacggc atttcatgaa
     1201 cgattaatgg agctggccag aggcagtatt gaaaaatcca gtaaggatga acgttattgt
     1261 ctgcattttt ctcctgaagc gcaaaaaata tttattgatc actataatgt tctggagcag
     1321 gatttgtctc cttccggacc cttaagccag tttcggggac atgtttcaaa aaaaacagaa
     1381 aacattgcga gaattgctgc gttattgcag tatttcagtc agggtgaagg gaaaatctct
     1441 gccgatatca tgacgtctgc tgtcgtaata agttcgtggt acacagatga atataaaaaa
     1501 ttatttgcgt tacctgatga atctgaactg cagctgcagg acgcccggga gctgctcgac
     1561 tggctcattg aggagtgccg cggagaatgc ccgccccgtg tcaggaaaaa ttatattctc
     1621 cagtgtggcc cgggccggtt ccggaacagg aaaaagctga acgtcctgct gaacattctt
     1681 gcatctcaac tcaggctctc tgtcgtgcag gaaggaaaaa ccacctatgt cctgctttcc
     1741 gaaataaaca atatcacgct gcctgaactg aatgcgtgct acagccagaa actgataaga
     1801 tggcatgagc aatcctttgg cttataatgt tccctgttgc tgagacggat tattcagtgg
     1861 tggtatcgca gtatgcccgt cacccccgct tctgcatgag ggtgacggag gaaaagtaag
     1921 tgtgcggagg cccgggagcg ttatgaatca acaggcgtat aacaggtact cgtcaatcat
     1981 cagttccagc tcgcgctttt cccgtttgcg cgccacagcc cagttttcaa gctctgcatg
     2041 tgatttatgc tttaactctt cagtccaggg ctgcatggag tgggcacaca tcagatcata
     2101 ccagcacagc catatcagcg tgtggtagag atcacgatcg tcgaaacgcc ggagcaggct
     2161 gttcagcctg tttgccagcc ggttttcacc cagacgatgt agctcactga gtaaaacgcc
     2221 gcccagatta aaatgtgtaa gcagcatgat ttcatgggtc tgaatgctgg tcattaaccc
     2281 ttcctgaatg gcaggaatga acggtggtgt tcccgggttg acgacctgat agagtccctg
     2341 tggtgccact gagatgatgg tgcctttttc tggcagaaaa aagtgggcca gggtcaggcg
     2401 gtgataacca tgaagatgcg gagcccccat tccatgagcg acgataagcc tcgtcacgtt
     2461 tgtcatccat ttctgatgct ggatgagcag cagctcacac cggttttcaa gtttttgcat
     2521 aatattctct cctttagcgc gcagcaaaat gcctccgggg gccggatcat gcctttgctg
     2581 cggttgaacg gataacggtc aggaattacg gacgggactg tgtaatacgt gcctgcaacc
     2641 aggcttccac ttcacttttc agccatcggg agctacggcc cagtttgatg ggggcaggaa
     2701 aggcaccatc cttgatgagc ttgtaaaacc atttatcggt caggccggtc agctgagtga
     2761 taaacgacat gtcgaccatc tggtcatcca tcagtgaaac tggggtattc atgaaaatgt
     2821 tcctccggat gttgttaatc tcaaccggcg ttggtgatga aaacaacgca ccaccttccc
     2881 cggcataaca accggggaaa aacgtaaaaa ataatcggga atgtgaccgt caggagataa
     2941 acggggggat tgcggtggct gagccgtacc ggtatgtcgg gtgattgttg cgaaacgatg
     3001 cttccgcacg cgcacgtgat aaaggaggga ggactttcgt gagacaaggg gaaaactggc
     3061 gttctgataa ttgcagagtt gtttttgaag gcggttgtgg tgcatcagcc cgtgacctgt
     3121 gggcaggcga caggtatcac tggtgggata agactgtctg attggtatta taaatatata
     3181 agagagctca aacacatgag ttcatatatg tgaaataaat ctgtgagtgt gatcagcaga
     3241 ccagtctacc cagtagcaac agattgcgtt gtactcacag ggttatccct gataactggc
     3301 gtataacctg cgtacataaa acgtacctgc aatgcacctg aacagagtgc attaagcatc
     3361 cgcctgagcg gtaaacgtat ccgatctggt gagtgacagt ctgagagcat tctcagtaat
     3421 cgcccccttg tatcctggaa attggttaag ataacagcga ttagatttgg caaggtgtat
     3481 gcagccagca gcttaacatc tcagctaact ggctggttac cttcaggttg tcgcaatggc
     3541 atcggacatc cggtgtcgga taactaaacg tagcgttaag gcgatattgc tgagagaccg
     3601 gttaagtggt tgcacgggag taatgagttt ggggagggta ccagaatgtc agtctggtgc
     3661 ggtatgctgg cagtatcacg ttgattacag cataaagtaa cgagagacga atggaagtta
     3721 aaaccagagc agttccgtcg ttgttcatcc cgggagacgc tggaaaaagt gatttcccac
     3781 acacgttata aacttacccc tgcggagctg gaagccttta actctgcggt cgat
//
LOCUS       DACADF010000261         3795 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.264, whole genome shotgun sequence.
ACCESSION   DACADF010000261 DACADF010000000
VERSION     DACADF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.264"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(42..2522)
                     /locus_tag="ISB30_004703"
     CDS             complement(42..2522)
                     /locus_tag="ISB30_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP1992245.1"
                     /translation="MLRMTPLASAIVALLIGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREMIKRLGINTDNFASGK
                     QCLTFKQLIQGGSYTWDIGVFRLDFSVPQAWVEELEIGYVPPENWERGINAFYTSYYM
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRFGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGANL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESKNYICQ"
     gene            complement(2538..3212)
                     /locus_tag="ISB30_004704"
     CDS             complement(2538..3212)
                     /locus_tag="ISB30_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAP1992246.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAEHKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(3293..>3795)
                     /locus_tag="ISB30_004705"
     CDS             complement(3293..>3795)
                     /locus_tag="ISB30_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1992247.1"
                     /translation="FMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSINMPTVP
                     TTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDT
                     VGYYLYLGDNSDVLDVSAPFNIESYKIADGQYAIPFKAKYLKLTDNSVNSGDVLSSLV
                     MRVAQD"
BASE COUNT         1048 a          933 c          706 g         1108 t
ORIGIN      
        1 ataaatagca ttatgcgaat ttccatagaa ccacctgact attactggca aatataattt
       61 ttactttcat caatctcttt accgaaggtg attgtgcatg aaagcccttg ttgcttatca
      121 attgccacat taaccgatgg cggtacttca ttggtgcgaa taaataactg gcttccctgg
      181 ccgacaacac caatattatg accatggata tcattgactt cataaccaaa cgttaatggt
      241 tgcccatccg ctcttaatgc ttttataaac catggcttgc gctgatcggt atcaaaatta
      301 accagtacaa ccgcgccgcg ataaggggcg gcaattttcc ggttgccacg taattctgct
      361 tcgctatcgc tttgtgacac atccaacatc aggtaatttt cccgataagg tgtcattccg
      421 tcgtatacca ccactccatt acggtttgtt gtgcgatatt tttgcccatt gacataagca
      481 tctttaattc ctggcgcatt catcacagca aacgtttcag aaagacggtt tgccagatta
      541 acgccacccg accaggcgac aatgccccct gaaacactgg ctccagcctg tcgataagca
      601 ctcgactgac tataactgcc attcactgtc gcaaccggcg cgttccaggt taaattcgcc
      661 ccagctgtcg tttcatttcc ctgatactga taactcaggt tgaccccata gttaaactga
      721 tcccggcttc caacggttcc tgataatccc gtattatttg aggcaacccc ctgatcatca
      781 aacgtcgttg agttagacat atatatttgc cgacgaggcg tcgtaacgtc atcaccccaa
      841 tcaaagggaa tcgatataaa aatattaaaa cgtttctctt catgatgatt ctcgtcataa
      901 gcatggcttg ccgcgagggt atagcttatc cttcgcaggt tgttggaata actcaactga
      961 taatccttac tgctgccgct acgcccccag taatctcgcc ataacgtact taaggacaca
     1021 gaaccccagc cttctggcaa tgactgactc atattggcag aaaagctatt tttacggcca
     1081 aaatcgttct ggtaataatc ggcaatgtca tagatatcgt tttcatcacg gcgataatta
     1141 tctttattgt ttgcccatac gtgatcatta aatgtccgat aatcacgcga cgaataacgc
     1201 caggccgcca gaccaaaacg tgtcgacgtt tggctcacaa atttgttgta ggcaatttga
     1261 taactttgcc cgtcaaacac atcgccgttg tcttgtttac tatgcgactt tgtggcatcg
     1321 acggaaatgg caccaaagcg tgtattccag ccagtcccca aagtgaacgc gtaataatta
     1381 ttcgcgacca tcgagccacc atacagcgtt aataagttat taaaaccata ctgataaccc
     1441 gcctggacaa aatcactttg tttgctcgcc ccttcaatat ggctacgacc cgccgcaaaa
     1501 tcatatttcg acacgccggg ttgcagcata tttggtaccg ctgcataagg caccagatag
     1561 gtggttaccg aaccgtccgc ctctttcacg ctaacatcaa gatccgctcc accaccggcc
     1621 aactgcaaat ctgtaatcgc aaacgggcct ggaggaacct ctttctgata aaccacaaaa
     1681 ccattctgtt caatagttac cagcgcgtta ctctgagcaa tcccctgcac tcgtggcgta
     1741 aaattttgtt tcgagttagg caacatctgc atatcacgaa acaaccgcac accgctgaag
     1801 cgaacagaat caaaaatatc gcttgatgtg tacatatcac ccacacgaag cgtgccgaga
     1861 agttgggcaa atccacgttc cagatacagg gtattgctct tccacacccc tggattgtta
     1921 tttgttttac tgaaactggc atcagaatgc agttgccacc ccaataaatt taacccgctg
     1981 ttaaaacgta catatgtact cttgctatta ccggacgctt tataatcgct gtaatactga
     2041 ctcatataat aagaggtata aaacgcatta ataccccgct cccagttttc aggtggtaca
     2101 tagccaattt ccagttcttc cacccaggct tgcgggacac tgaaatcgag acgaaaaact
     2161 ccgatatccc aggtatagct cccgccctga ataagttgct taaatgttaa acattgctta
     2221 ccgctggcaa agttatcggt attaatgcct aaccgcttga tcatttccct tgataaacat
     2281 gtttcttgcg ggttgtcttt aacaatgatc tcatatttcc cacgccattg cttattgaca
     2341 taaatatcga tgtcatactg ccccggcaag ggttgattat catcaagacg aatatttgca
     2401 acctgctggt ctttcattcc acctatcatg aaatgggtat caaaggtttc ttcagctgca
     2461 taagcttcaa tgccgatcaa taacgctacg attgctgatg caagtggggt cattctcaac
     2521 ataggcagct cctgcaatta aattttgtca ctaatatagt tgccatggtc atcgataatg
     2581 gtcagatgcc agttatttgc attattactt ttgacattaa cactctgact ttctaagggg
     2641 gcaatcataa tagtttcata attgactttg acattattag ctttgacatc cgaaatcgtc
     2701 acccaattag ctgagtcatt ttttatcacc aatccattgc cactgcgatt taccagtaac
     2761 tttttaaagg tcgctttatt taccggagca atacccgctg gccggtaaaa caacttaatt
     2821 ctgttttgca tcgcaaactt cagtgcattc ttaccttctt gttctggact atttggtgga
     2881 atatccagaa cattcagata aaaaatactc tctttattag tgggtagttt attcggcata
     2941 attttaattt ttacttgctg cccggaattt gcccctattt ttgccactgg tggcgttaac
     3001 atgaaaggaa cctgaatttt ttctggtggt aatgacgtat cgccatcatc aatccacgcc
     3061 tgcatcagcg aagaacgatt tccctggttc atcagctgca ccatcacttc tttgtgctct
     3121 gccgggtaaa taatacgcgt cccgtagata acaataccgg catgtgcagg aaggaccata
     3181 gaagaaatga gtaaagctaa taatcctttc atcttaatgc ctcttaaatt aaaaggccgc
     3241 catggggata gcggccatgt taattacctg aagaatcttg caattttatt tattaatcct
     3301 gcgccacacg cataaccagg gaagataaca catcacctga attcactgag ttatctgtca
     3361 gtttcaggta ttttgcttta aacggaatgg catattgacc gtccgctatt ttataactct
     3421 cgatgttgaa aggtgcagaa acatccagga catcgctgtt atcacctaaa tagagataat
     3481 aacccacggt atcgttgcta ctggctatcg ccttatcgtt tccggttatt ttatcgttgg
     3541 taatcaacga gagttttaca ccagcaactt ctttcggaca gctaattttt aacatgctgc
     3601 tggttacacc gactgcatca tcataggtcg agtatttagc tttaccttca aagacagtgg
     3661 ttggtacagt tggcatatta atacttactg aatcgccacc cgcttcgaat ttacacggag
     3721 attctgcaat attcccttta atggttaatg ttccggtatc aacgtctgcg gcaaataccc
     3781 cagcgctcat aaaaa
//
LOCUS       DACADF010000262         3766 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.265, whole genome shotgun sequence.
ACCESSION   DACADF010000262 DACADF010000000
VERSION     DACADF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.265"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            14..1399
                     /gene="dcuC"
                     /locus_tag="ISB30_004706"
     CDS             14..1399
                     /gene="dcuC"
                     /locus_tag="ISB30_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAP1992248.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1440..2120)
                     /gene="dpiA"
                     /locus_tag="ISB30_004707"
                     /gene_synonym="citB"
     CDS             complement(1440..2120)
                     /gene="dpiA"
                     /locus_tag="ISB30_004707"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HAP1992249.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2089..3747)
                     /gene="dpiB"
                     /locus_tag="ISB30_004708"
     CDS             complement(2089..3747)
                     /gene="dpiB"
                     /locus_tag="ISB30_004708"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HAP1992250.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELYLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          865 a          986 c          912 g         1003 t
ORIGIN      
        1 gacaggaaaa attatgctga cattcattga actccttatt ggggttgtgg ttattgtggg
       61 tgtagctcgc tacatcatta aagggtattc tgccactggc gtgttatttg tcggtggcct
      121 gttattgctg attatcagtg ccattatggg gcacaaagtg ttaccgtcca gccaggcttc
      181 aacaggctac agcgccacgg atatcgttga atacgttaaa atattgctaa tgagccgcgg
      241 cggcgacctc ggcatgatga ttatgatgct gtgtggcttt gccgcttaca tgacccatat
      301 cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca ttgcagtata ttaactcccc
      361 ttaccttctg atgattgccg cctattttgt tgcctgtctg atgtcactgg ccgtctcttc
      421 cgcaaccggt ctgggtgttt tgctgatggc aaccctgttt ccggtgatgg taaacgttgg
      481 tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg gcggcgatta ttctcgcacc
      541 gacttcaggg gatgtggtgc tggcagcgca ggcttccgaa atgtcgctga ttgacttcgc
      601 cttcaaaaca acgctgccta tctcaattgc tgcaattatc ggcatggcga tcgcccactt
      661 cttctggcaa cgttatctgg ataaaaaaga gcacatctct catgaaatgt tagatgtcag
      721 tgaaatcacc accactgccc ctgcgtttta tgccattttg ccgttcacgc cgatcatcgg
      781 agtgctgatt tttgacggca aatggggtcc gcaattacac atcatcacta ttctggtgat
      841 ttgtatgcta attgcctcca ttctggagtt catccgcagc tttaataccc agaaagtttt
      901 ctctggtctg gaagtggctt atcgcggtat ggcagatgca tttgctaacg tggtgatgct
      961 gctggttgcc gctggggtat tcgctcaggg gcttagcacc atcggcttta ttcaaagtct
     1021 gatttctatc gctacctcgt ttggttcggc gagtatcatc ctgatgctgg tattggtgat
     1081 cctgacaatg ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt atgcgtttgt
     1141 tgagatgatc ccgaaactgg cgcactcctc cggcattaac ccggcgtatt tgactatccc
     1201 gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc gtttctggcg tagtcgttgc
     1261 ggttgccggg atggcgaaga tctcgccgtt tgaagtcgta aaacgcacct cggtgccggt
     1321 gcttgttggt ttggtgattg ttatcgttgc tacagagctg atggtgccag gaacggcagc
     1381 agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt ttcctccggc tttatcatgt
     1441 cacccactgt ggtatatgcg ttgcggtctg ccaactttgc cgtgaacaat ttcagcaata
     1501 atcagatggc ggctggcgca atattcaaga taacgcctgg cagtggtgcg actgatggtc
     1561 agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag gctctttgaa cagttttcgc
     1621 accgcgttta gcgtcaaggg gtcaatgccg gtcggcagtt cgtctttggg ttcaccgcga
     1681 gcataggcat taaacatctc atcaatttgc ttctggctgg cgctatcaat actttccagc
     1741 atatgtttac gctggcggaa acgggttagc gtttgcccca gacgttcata ggcaattggc
     1801 ttaatgagat aatcaaatac accacaacgt acggcttcag acaccgtttc catatcgctg
     1861 gctgcagtgg taaacaccac gtcgccggga taatgcgcct gcaccagttc atgcagtaaa
     1921 ttaatccctc taccgtcagg aagatagtta tcgagcaaga ttagccccgg cttaaaacgc
     1981 tcgatcatca ttcgggcctg cgccaggttt cccgccagta atatctggct gaatccggga
     2041 atgtgacgaa tatattccgc atgcatctct gccagcggcg tttcgtcctc aacgatcaat
     2101 agggttaatg gagctgtcat taggtttcac tttcggaata tagattgaaa ataaggtacc
     2161 gcagggatca ttatcttcga gagtgataac accaccgcag cgcgttacgt agctggcaat
     2221 caagtacaac ccaatgccat gttcaccggg ctcgtcagca cgcgtactga caccctgctc
     2281 aaatattttg tctcgtagag actctggaac gccgcagccc tgatcggcga cttcaatcac
     2341 cacatcatcg ccttcatcgc tgaggtataa ttcaacaatc ttgtttcctt catcgctacg
     2401 caggcttgct tcgaaggcgt tatcaagtaa attgccaaca atggctgcaa actcggtgct
     2461 gtccagtcct ggcggcagtt gcgaaagctg gctaccgggg acgatgacca tttttagccc
     2521 cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc gccacctggc gatcggcaaa
     2581 cgcctcacgc aggctgtcaa taagctgttg ctgggcctga gactcaccct gcaccatcgc
     2641 cagcacgcga tcatactctt tcatctgcaa cagaccattg agcgtcgaca tccaattgag
     2701 atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt ttaatttgcg tcagttgcgc
     2761 attgagggtg gaaatttcgt ctttactacg aaagctgata atggccccca gcaaatcatc
     2821 gccagaacga atagcttccc gattggcgat aacgctcaga ccgttaaagt tcgccaccac
     2881 atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg gcgggcctga ccacttcagc
     2941 aatgggttta cccaaccatt gccgtccggg ggagctcagc cccagcatct ttcttgcgtt
     3001 acgattgatg gcggtaatgt acccgtgcgg atccaccgca atcagccctt cataaaccga
     3061 actaaacagc gcctcttgct gacggaccac gcgtgcgatt tgctttggct ccatgcccat
     3121 catctgccga cggatatgcg cggccaggaa ccacgatagc aacatcaaaa tccctaacag
     3181 cacgacaaac acgcctgcca tcggtaataa aaactcagcc cgccagctat cgattttact
     3241 caccagatag ccaatcgaca ccacaccgat gacttttcca tcgtcatcaa agattggcgt
     3301 tttggcacgc atcgccatgc caattgaccc tttcccggtg atgaagtagc tctccccttt
     3361 ctccagcgcg cccggtttgg tgaactgcat aggataacca attttctccg gattaggatg
     3421 gtaaagacga atcgagtgcc ggtcgccaat caccacataa tcgaaatcag tatctctttg
     3481 taatttgtca gcgatggtcg ccagcctctt gtagtcacgc gttttcactg cgctgatgat
     3541 actgtcatta gaagcaataa tttttgcctg attcattgcc atgtcgcgca catgaagcgt
     3601 taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg gctgcaatga caaatattga
     3661 gaacaccaga atcagcaaaa agatacgcag cggaaatgcc agtctttgga aaaatgcaaa
     3721 ctgtttattc tcgttaagct gcaacattgt tttccttgcg ggttgt
//
LOCUS       DACADF010000263         3750 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.266, whole genome shotgun sequence.
ACCESSION   DACADF010000263 DACADF010000000
VERSION     DACADF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.266"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..2997
                     /locus_tag="ISB30_004709"
     CDS             <1..2997
                     /locus_tag="ISB30_004709"
                     /inference="COORDINATES: protein motif:HMM:NF014452.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107086997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1992251.1"
                     /translation="MTVNNKNIAINDKITAIELTKSNKNKDLKFHGGGSIELTDNLNS
                     GTGGLIFDEGQHYSVIGKDKAYKGAGVEIGKDTVVDWSVKGEANDNLHKTGAGTLNVN
                     VAQGNNLKTGDGTVFLNAEKAFNAIYVASGRGTVKLGQADALDKNSDYRGIYFTSRGG
                     TLDLNGFSQSFKKIAATDVGTIITNTSDKTATLSLQNPSRYVYHGSITGNTNIEHTGT
                     QKSADSSLIIDGNINTRNDITVRNSQLRLQGHATSHAIFREGPRHCYVPGVLCDKDYV
                     TDFARLESEANKKNNSAYKTNNQVASFDQPDWETRHFRFKTLNLENSEFTTARNSVVE
                     GDIVASNSTLKLGGDVPVFIDMYDGINITGNGFGFRQDVREGRSADDGSSSYTGKITL
                     QKGSTLDINNRFIGGIEAHDSKVNVTSPDALLQNSGVFVNSTLSVRDGGHLTAQKGLY
                     SNGRVQIGKKGTLSLSGTPENGADNTWMPVLTYMTEGYDLTGDNATLNISQQAHVSGD
                     VHATSSSSIRIGSENPGSVSSSVSPVLAAGLFSGYNAAYYGAITGGKGNVSMNNGLWQ
                     LTGDSDINSLTTRNSRVQSEENGAFRTLTVKTLDATGSDFVLRTDLKDADKISITEKA
                     SGSDNTLNVSFMKNPSPGQSLNIPLVSAPAGTSGDIFKAGTRVTGFSRVTPTLRVDTT
                     GGSTKWILDGFRTEADKAAAAKANSFMNAGYKSFMTEVNNLNKRMGELRDTNGDAGAW
                     ARIMNGAGSADGGYSDNYTHVQVGFDKKHALDGVDLFTGVTMTYTDSSADSDAFSGKT
                     KSVGGGLYASALFNSGAYIDLIGKYIHHDNDYTGNFAGLGTKHYGTHSWYAGAETGYR
                     YHLTEDTFIEPQAELVYGAVSGKTFRWKDGEMDLSMKNKDFSPLIGRTGIELGKTFRG
                     KDWSVTARAGTSWQFDLLNNGETVLRDASGEKRIKGEKDSRMLFNVGMNAQIKDNMRF
                     GLEFEKSAFGKYNVDNAINANFRYMF"
     gene            3211..3483
                     /locus_tag="ISB30_004710"
     CDS             3211..3483
                     /locus_tag="ISB30_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1992252.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT         1075 a          809 c          998 g          868 t
ORIGIN      
        1 atgacagtta ataataaaaa tattgccatt aacgataaaa taactgctat tgaactgacc
       61 aagagtaata aaaataaaga tttgaaattc catggtggcg ggagcattga gctcaccgat
      121 aacctgaact caggaaccgg aggattgatt tttgatgagg gacaacatta ttcggttatt
      181 gggaaagata aagcctataa aggggcgggt gttgagatcg gaaaagatac ggttgtcgac
      241 tggtcggtaa aaggggaggc aaacgataac ctgcacaaaa caggggccgg gacactgaat
      301 gtcaatgtgg cccaggggaa taacctgaaa acaggtgacg gtaccgtttt tcttaatgca
      361 gaaaaggctt tcaatgctat ctatgttgcc agtggccgtg gaacggtcaa actggggcag
      421 gccgatgcgc tggataaaaa tagtgattac agaggtattt attttactag tcgtgggggg
      481 actctggatt taaacgggtt cagccagtcg ttcaagaaga tcgcggcaac tgatgttggt
      541 accattatca ccaatacttc tgataaaaca gcgacccttt ccctacaaaa cccctcccgc
      601 tatgtctatc acggtagtat cacgggaaat acgaatatcg aacacactgg gacacagaaa
      661 agtgctgaca gcagtctgat tattgatgga aacattaata cacgcaatga cattactgtg
      721 cggaattccc agctcagact tcaggggcat gccacatcac atgcaatatt ccgcgagggg
      781 cctcggcact gctatgtccc cggagttctt tgtgacaaag attatgttac tgattttgcc
      841 agactggaaa gtgaggcaaa taagaaaaat aacagtgcct ataaaacaaa taatcaggtg
      901 gcttcttttg accaacctga ctgggaaacc cggcatttcc gatttaagac tctgaatctg
      961 gaaaactcag aattcacaac tgcacgtaac tcagttgttg agggtgatat tgtcgcatcg
     1021 aattcaacgc tgaaactggg gggcgacgtt ccggtgttca ttgatatgta tgatggcatc
     1081 aatatcaccg gtaatggttt tggcttccgc caggacgttc gtgaaggacg ctcagcagat
     1141 gatggcagta gcagctatac aggcaaaatt acactgcaaa aaggctccac gctggacatc
     1201 aacaaccggt tcattggcgg tattgaagcc catgacagta aggtaaacgt cacctcaccg
     1261 gatgccctgc tgcagaacag tggtgttttc gtgaattcca ccctttctgt ccgtgacggc
     1321 ggtcatctga cggcacaaaa agggctctac agtaacggcc gggttcagat tggaaagaaa
     1381 ggtacgcttt ccctgagcgg cacgccggaa aatggcgctg ataatacctg gatgcccgta
     1441 ctgacataca tgacagaagg ctatgattta accggtgata acgctacgct gaacatcagc
     1501 cagcaggcgc atgtttccgg ggatgttcat gcaaccagtt catccagcat tcgtattggc
     1561 tccgaaaacc ctggctcagt ttcctcttct gtctcccctg ttctggctgc cgggttgttc
     1621 agcggatata acgcggcgta ctacggtgcc atcaccggcg gtaaggggaa cgtcagtatg
     1681 aataatggcc tgtggcagct gaccggagat tccgacatca acagtctgac gacccgtaac
     1741 agccgggtac agtctgaaga aaacggtgcc ttccgtaccc tgacggttaa gacacttgat
     1801 gccacgggca gtgattttgt cctgcgcact gatctgaagg acgctgataa aatcagtatt
     1861 acggagaaag ccagcggttc agacaacacc ctgaatgtca gctttatgaa gaacccgtct
     1921 ccgggacagt ccctgaatat cccgctggtc agtgcaccgg ccggaacatc aggggatatc
     1981 tttaaggccg gcacccgggt gacaggcttc agtcgtgtga cgccgacgct gcgtgttgac
     2041 accactggcg gcagtacgaa gtggattctg gatggtttca ggacggaagc tgataaagcg
     2101 gctgcagcga aggcgaacag tttcatgaat gccggctaca aaagctttat gacggaagta
     2161 aacaatctga acaaacgtat gggggagctg cgtgacacga acggtgatgc cggtgcctgg
     2221 gcccgtatta tgaacggcgc cggttcagcc gatggcggat acagtgataa ctacacccac
     2281 gttcaggtcg gttttgacaa aaaacatgcg ctggacggtg ttgacctgtt caccggtgtc
     2341 acgatgacct ataccgacag cagtgcagac agtgatgcgt tcagcgggaa gacaaaatcc
     2401 gtggggggag gtctgtatgc ttcagcactg tttaactccg gtgcctacat tgatttgatt
     2461 ggtaaatata ttcaccatga caatgactac acaggtaact ttgccggtct ggggacgaag
     2521 cactacggaa cccactcctg gtatgccgga gcggaaacgg gttaccgtta tcacctgacg
     2581 gaagacacat ttattgagcc tcaggccgaa ctggtttacg gcgcagtgtc cgggaaaaca
     2641 ttccgctgga aagacggtga gatggacctg agtatgaaga acaaggattt cagcccgttg
     2701 attggcagaa cagggattga gctgggcaaa accttcaggg gtaaggactg gagtgtgaca
     2761 gcccgtgccg gaaccagctg gcagtttgac ctgctgaata atggcgagac agttcttcgt
     2821 gatgcatccg gagagaaacg gatcaaaggg gagaaagaca gcaggatgtt gttcaatgtt
     2881 ggcatgaacg cacagataaa ggacaacatg cgctttggtc tggagtttga gaaatccgcc
     2941 tttggtaaat acaacgtgga taacgcaata aacgcgaatt tccggtatat gttctgacaa
     3001 ggagaggccc ctgaagaggg gcctgtagtg atgttactcc cgggttaatg cagctagtaa
     3061 aggtatggct ctgactaccc tggaaagtgg tgcccatcgc atcactacaa attctggcaa
     3121 ttgcgtacgt ggtaacggca agttatttaa cctcaaacgt acgtgcccga gcggtgcgct
     3181 gaaataccgt cataaataag cgagggtaaa atggaaatac gcgaaggcca caataaattt
     3241 tacattaatg acgaacaggg caagcaaatc gctgaaattg tctttgtgcc gaccggagag
     3301 aatttagcga ttatcgaaca taccgatgtc gatgaaagcc tgaaaggaca agggattggt
     3361 aaacagctgg ttgcgaaagt cgtggaaaaa atgcgtcggg aaaaacgaaa aattatccca
     3421 ctatgcccat ttgcgaaaca cgaatttgat aaaacacggg agtatgatga tattcgcagt
     3481 tgatgggaga gtacagagtc acgatatttt tcatgctctc cgcggtgtga tgcaggagag
     3541 catctgaagg gtaggagatg cacaaagaat ggtcagagag agcgtttttt tgtcccaagt
     3601 catccccttt actgagcaaa aaaaaaagaa tatctcctat atgagaatca tcattcgggg
     3661 ttaataagtt ttgcgtcccc agagcgttca atattgatag gagtcatatt atggaaggta
     3721 aaaacaagtt caatacttat gttgtttctt
//
LOCUS       DACADF010000264         3704 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.267, whole genome shotgun sequence.
ACCESSION   DACADF010000264 DACADF010000000
VERSION     DACADF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.267"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            174..1412
                     /gene="alaC"
                     /locus_tag="ISB30_004711"
     CDS             174..1412
                     /gene="alaC"
                     /locus_tag="ISB30_004711"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAP1992253.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(1477..1548)
                     /gene="ypdK"
                     /locus_tag="ISB30_004712"
     CDS             complement(1477..1548)
                     /gene="ypdK"
                     /locus_tag="ISB30_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAP1992254.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1905..2825)
                     /gene="lpxP"
                     /locus_tag="ISB30_004713"
     CDS             complement(1905..2825)
                     /gene="lpxP"
                     /locus_tag="ISB30_004713"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAP1992255.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GTMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3178..3420
                     /locus_tag="ISB30_004714"
     CDS             3178..3420
                     /locus_tag="ISB30_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAP1992256.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3497..>3704)
                     /locus_tag="ISB30_004715"
     CDS             complement(3497..>3704)
                     /locus_tag="ISB30_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992257.1"
                     /translation="FSISGGGSEASYKETEKTSVMTTTHSTKLQPSQAILFKMREDAP
                     PLNLTEDITPTYPTKANYLIHPLR"
BASE COUNT          922 a          877 c          947 g          958 t
ORIGIN      
        1 gttagcgtag ataaatttcc tgtttttgaa gtggagttcc tcatttaaag ccattgataa
       61 aaatgttcta aaacgctgga cagacagaaa ttaatcaggc tattttcaaa acagccacca
      121 tcgtggcagc gtcgctcgga cggtccgggc gctaacgtta atctgaggaa attatggctg
      181 acactcgccc tgaacgtcgc tttacgcgca ttgatcgtct cccgccctac gtttttaaca
      241 ttaccgctga actgaaaatg gctgcgcgtc ggcgcggcga agatattatc gatttcagta
      301 tgggtaaccc ggacggtgcg actccgccgc atatcgtcga aaaattatgt acggtggccc
      361 agcgcccgga cacgcatggt tactccactt cacgcggcat tccgcggtta cgtcgcgcca
      421 tttcccgctg gtatcaggat cgctacgacg ttgaaatcga cccggaatca gaagccatcg
      481 tcactattgg ttcgaaagag gggctggcgc acctgatgct ggcgacgctg gatcatggtg
      541 acacagtgct ggtgccgaat ccaagctacc ccattcatat ttacggcgcg gtgattgccg
      601 gggcgcaggt acgctcagtg ccgctggtgg aaggtgtcga cttcttcaat gaactggagc
      661 gcgccattcg tgaaagttat ccgaaaccga agatgatgat cctcggcttc ccgtcaaacc
      721 caaccgcgca atgcgtggaa ctggaattct ttgagaaggt tgtggcgctg gctaaacgct
      781 acgatgtgct ggtggtccac gacctggcct atgccgatat cgtctacgat ggctggaaag
      841 cgccgtcaat catgcaggtg ccgggcgcac gcgacgtggc ggtcgagttc tttacgctgt
      901 cgaaaagcta caacatggcg ggctggcgta tcggctttat ggtcggtaac aaaacgctgg
      961 tcagcgcgtt ggcacgtatt aaaagctatc acgattacgg cacctttacg ccgttgcagg
     1021 tggcggctat tgcggcgctg gagggcgatc aacagtgcgt gcgcgatatt gccgaacagt
     1081 acaaacgccg ccgcgatgtg ctggtgaaag ggctgcatga agcgggctgg atggtcgaaa
     1141 tgcctaaggc ttcgatgtat gtctgggcga aaatcccgga accgtatgct gccatgggct
     1201 cgctggaatt tgccaagaaa ttgcttaacg aagcgaaggt ctgtgtctct ccagggattg
     1261 gctttggcga ctacggcgac acgcatgtgc gctttgcact gattgaaaac cgcgaccgta
     1321 ttcgtcaggc gattcgtggg attaaagcga tgttccgtgc cgacggttta ctacccgcca
     1381 gcagcaaaca tattcacgaa aacgcggaat aaacgccttc gccagaaatg caaacaggag
     1441 cctaatggct cctgtttttc attgcacagt ttgcttttag atcatcaggg caaaagtacc
     1501 ggcccaaacg atgaccataa aagaaatgcc cataaagaaa tatttcactt tacatctctc
     1561 cgcgtaatta ctgttacgcc tgaatgactt cattacacct gatacagtga gttgacaccg
     1621 ggcaaaacga ccttaaacgt cctgtatttt accgtgggca tcatcccaga attctgagct
     1681 aaactgctcg agacggcgct aatgtacgcc taaaactgaa acgagacaag aggcattttc
     1741 ttatttactt ttagtaactt acaggtgtct catatcggcg acagtttaat ttctgacggt
     1801 aataattaga tattcagcga aggaaaaaac cttaagaaaa aaagccataa aaaccatgag
     1861 gttattatgg ctgatttgag gagggaaaga gtgagagccg tttgttaaat gtataacgac
     1921 gattcaccca ccgggcgcgt tttaaagcga cggtgtatcc agaggtactg ctccggtgcg
     1981 cgcatgatct ctttctcgat aatcttgttc atataggcag cggcttgatt ttcatctgtc
     2041 gggtagcctt ccatctctgg ggtgatgtac aaacgatatc cgctgtaatc cgcttttctt
     2101 accatcgtta cggtcaacat ggctgcgcca gagagacgag agaggacata ggtgccattg
     2161 gttgtggcga cattttccac cgcaaagaac ggcgcgaagg agctgccttt acgaccataa
     2221 tcctgatcgg gagcaaacca taccgcttca cctttcttca gtgcactgac aatgccgcgc
     2281 agattatttc tgccgatcat cgctttgtta gagcgcatgc gaccacgggt ctggacccat
     2341 tccatcagct gattattatg cgggcgatag gtggccatca ttggttggca aagtcccatc
     2401 acccgaccgc ccagttccag cgacataaaa tggacgccga caaccattac gccgcgattt
     2461 tgcatttgtg cgcgtttaag gttatccagc ccttccacat caaaccattt acgtacgcga
     2521 ctgtcgggcc agaaccacgc catgccggtt tctaccagcg ccatgccgag tgaacgaaag
     2581 ttttcggcaa tcatcttctc gcgctcttcc gcagaatgct gcgggaaaca aagttccagg
     2641 tttttacggg cgatagattc acgacgtttc aggaatggtc gcgccattgt gccgatacgc
     2701 gtgccgagaa agcagagaac aggataagga agctgtaccc aaagccagag tacaccaagc
     2761 ccaaaccacg tgagccagta gcgaggatgc agaaactcgc gggaaaattt gcattttgga
     2821 aacatagtta ccttctgtag atacgaattg ttgtatcgca gaagattcca tgtcgacagc
     2881 ggtacactca attgattagg ccatcatgta cgacaatggt tcctgtaaag agtgtaaaga
     2941 ctagtcagga cataaccacg aggagcatga atgcggtatc tgtactttat cactcgctga
     3001 ctgctttcag ctactgatta ttaatcgtgc aaagtaaata tttttccgta cgtaacagtc
     3061 ttgcatttaa catgctgttt tgattgatgc ttagcaaaaa ttgccagagg ttaaggattg
     3121 tcttactatt aaagtgactg aagttacagg ttttttagcg cggcaggagg ttgagctatg
     3181 atttatttat ggatgtttct cgccctgtgt attgtctgcg taagcggata catcggtcag
     3241 gtactgaaca tggtatcggc gatctcttct ttcttcggca tggtgatcct cgctgcactc
     3301 atttattact tcaccatgtg gttaactggc ggtaatgaac tggtgacggg gatatttatg
     3361 tttcttgccc cggcttgtgg cttgatgatt cgctttatgg tggggtatgg caggcgatag
     3421 tgggtcagga tggctcattt gggcaggtgt tcacatcagc atcagacatg attgttcacc
     3481 tgccaggtga cagacgctat cgcaaaggat gaatgagata attcgccttt gtcggataag
     3541 taggcgtgat gtcttctgtg aggtttaatg gtggtgcatc ttctctcatc ttaaacaaaa
     3601 tcgcctgtga tggctgaagt tttgtagaat gcgttgtcgt catcactgac gttttttctg
     3661 tctctttata gctcgcctca ctaccaccac cagaaataga aaat
//
LOCUS       DACADF010000265         3699 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.268, whole genome shotgun sequence.
ACCESSION   DACADF010000265 DACADF010000000
VERSION     DACADF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.268"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..336
                     /locus_tag="ISB30_004716"
     CDS             <1..336
                     /locus_tag="ISB30_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1992258.1"
                     /translation="PVHVIIFSPFNIKRCLGKVPVTFVPRTIAIIDFVALINGSYCSV
                     PEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNLKRINELV
                     RHQHINYLV"
     gene            complement(393..1421)
                     /gene="chuS"
                     /locus_tag="ISB30_004717"
     CDS             complement(393..1421)
                     /gene="chuS"
                     /locus_tag="ISB30_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HAP1992259.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGHVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(1470..3452)
                     /locus_tag="ISB30_004718"
     CDS             complement(1470..3452)
                     /locus_tag="ISB30_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HAP1992260.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT          944 a          947 c          852 g          956 t
ORIGIN      
        1 cctgtccatg taattatttt ctcccccttt aatattaaac gctgcctggg gaaagtaccg
       61 gtgacctttg ttccgcgaac tatcgccatc atcgactttg tcgcactcat caatggcagt
      121 tactgctctg tgcctgaagc ggatgtgtcc ctctcgcgca agcagcatca ggttctgagc
      181 tgtattgcga atcaaatgac aacggaagat attctggaga aactgaaaat atcgctaaaa
      241 acgttctact gccataaaca caatatcatg atgatcctca atcttaagcg gatcaatgag
      301 ctggtacgcc atcagcatat taattatctg gtgtgaacga ttgatcaata taaagaggcc
      361 cagcaacagc cagacctccc gttaattata cgttatgcag taacgccttc cggtatcaac
      421 gaagcaattt gcttacgcca ttgcgcttgc tcctgttcac cttctgtacg ttgaccataa
      481 agttgcgcta tctgcgtacc atcatgggca aacaattcca gactggttac gtgaccatcg
      541 ctggtcggtt tacgggtaac ccaggcttca gcaatgctct cttctaatag atgaagagta
      601 aacgtcgggt tgaaaatatt cagccaacct ttcattggca ccactttttc taccacaccg
      661 gtgaaaatct gtacgcagcc acggttgcca acaaacacca tgatttcatt gccatcctgc
      721 tgtgcagatt caagaatttg cgccaacgca ctgttggata ctttgcaggc caaatcgtct
      781 gccaccagat tgaacgcctg ttggcgcgtc agattgtgac gcttgagcaa cgtaaaaaac
      841 tgatgaacgt cggtcatcgc ccgccactct tgctcgacca cactggcatc ggctcgcgtt
      901 tgaacaactg gcgcatcaac ggcttttaac tcaagcggcg tattctcatc ggtgataaac
      961 cgtgccagaa gctcactcca tgccgccata tcggtattgt cggtggcata aacttttagt
     1021 aatgcatcgc cctgatgatc aaagaactga atactctggc gttcgccacg ggccgtgttt
     1081 tctttgatgt ggaaaacact ggcccattga ttgagaaaca gacgtaaatc cagcgcgcgc
     1141 ggattgagga tcaatccggc atgtccgttc aaatgctggt ttgtgaacgc accaacttgc
     1201 tcatggactg catattcatt acggcaaata catttggttt cgccaacact ttcgagcgcc
     1261 gccagaattt cacgaatatc gccgcgcatc cgccacgcgt cgtgcgtgac gcgcgcaaat
     1321 gccagttctg cttcgctgat attcattaaa cctgcgatgt cacgcgcgta ctttccggga
     1381 ttttgttctt ttaactcaag ccagcgtgtg tagtggttca ttgtctctct tccttccaga
     1441 taaatgcccc gaaatatcgg ggcagatgat taccattgat aactcacgaa aatttttccg
     1501 ttacgaccat cctgtgggat gccttgcggc gaccagtact ctttatcgaa ggcgttgccc
     1561 aataccagag tagtggtcat gcctttgagc gcctgttgtc cttgataact gacgtagaaa
     1621 tcattcacac catagccagg ttgtttgctg tagctgctgc tgatatgtgt tgagcgatcg
     1681 gcaaacgtac cgacccaacc aacagagaag ccactgtgag cgatcggaat attcagggta
     1741 ctggtaacgg tatccgggtt aatgctggag atatattccc cggtatcggt gtctttgccg
     1801 cgggtacggt tataggccac atcaaggcta aacagatcag tggtatattt cgtcatcaca
     1861 tcccagcccc agattttggc gttcgggacg ttatacgaca tagttgtcgc cgccgcgaaa
     1921 tcgacggtcg tggagatata atctttcgct ttggtatcaa agtagctggc tttaaattcc
     1981 agagcatcat tggacaacat caggtcatca aaacgtagcc caaaaccgta ctcctgagtt
     2041 tcgttagttt ccggacgtaa gttcgggttt ggcacccaat agttggtata gaagcgacca
     2101 atcgagaagt gtttagaatc attatacatt tcgcccatcg tcggggcgcg gaatgcctga
     2161 gcatatgagc caaataacat cagccagttg gtcgggttga tagtcatccc cgcacgagat
     2221 gaccatttgt cggcatcaac atctttgtag ccgtcgctgc taccgcgata actgtcatag
     2281 cgggttccgc caagcagggt aatcggcaga tcgcgtaagg tgatctcatc ttgtagccag
     2341 ccagagctaa aatcgatttt tgcttgcggg aagcccgtcg tcgcgccacc cggatgttgt
     2401 tcctgacgat aatactcacc gccatatgtc agtaagtgag aagcgaaact gtcggcaaat
     2461 agagtggaac ggttctccag ccttgctcct tttgttatct gttcacgata ctcgcctgaa
     2521 ctccccgtgt tttgcgcatt aatacggact tccgaccagt atatttttgc atctgcattt
     2581 aaccagtcgt taccctgcgg ggcgagttta taagaaagct gcgcatcgcg ttgaattgtt
     2641 gaacgatcga ccatcgggtt gctgctatca gaagcttcaa cagtctgcgg attttttggt
     2701 tcacgcgcgt cgttgttgta gtaacgcact aaaccgctca gagactgggc tgaatcaatt
     2761 tgccaggtcc ctttcgccag catgttatta atggattcgt cattcggcgc ggtttcacca
     2821 ttgctctggc gtaaatcacc ccgatcgcga ctggaccagg ccacaatacc atccagattt
     2881 tcagttcgcc caaacgcgct cgcgcctaat cccaggctat ggtcccccgt gccgccagta
     2941 ccaaagacac gaaaaccact gctttgtcct tcctgcaata aatcttttgc atcgaccgta
     3001 tcgtaggaga tcactccacc cagcgcgcca ctgccataca gtaatgctga aggtccgcga
     3061 acaatctcaa cacgcttgat cagcgccgga tcgagaaaag tgccattcag gtgtccggta
     3121 tccgttccct ggcgaacacc atcgacaaga accagcacgc cgcgatgatc atagccacgc
     3181 atatttacat cctgaccgtt ggttcgtccg gtaccatcaa gagtaattcc aggaacatga
     3241 cgcagcaaat cagtggctga agtagcagtt tgattttcag gagcggaagt gtcgataacg
     3301 ctgaccatca taggcgcttc gaaggaacta cgtgcattcc ccgttgccgt aacggtcatg
     3361 gtttcagtag caaaagcaaa cgttggcaag gtggcagaaa cagccaaagc caacaaactc
     3421 aaacgcaacg aagtaaattg cggacgtgac atagcgattc tccatgagga aaataaaaaa
     3481 tgctggctgc ctgggttaat accggggtgg ctggcgaaaa ataaatttat ttacaattca
     3541 aatgattaga tatcaccacg ggaagctatt agcgtatata tcccgtaaaa ttgccgaata
     3601 tttctgataa ttcagcaaaa gcacgccctc tttgtcaggc aaagtagtac gccacgtctg
     3661 aagtgaagag tgctgtgcgt tttcccttac aaaagatat
//
LOCUS       DACADF010000266         3638 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.269, whole genome shotgun sequence.
ACCESSION   DACADF010000266 DACADF010000000
VERSION     DACADF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.269"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            13..>3638
                     /locus_tag="ISB30_004719"
     CDS             13..>3638
                     /locus_tag="ISB30_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1992261.1"
                     /translation="MKITVLNQRYKKHFGYPHILMGDVKVTVSAMIACAVLLHPNKLF
                     ASVAGTSLPYQTYRDFAENKGEFRPGAENVPLYDKNGNPVTTLNKAPMIDFSSNDSTG
                     VATLVSPQYVVSVKHNVGYQYVKFGYADDSSYALVDRNNHWRDFHTPRLNKIVTEVTP
                     LDITNAGTAKGTYQNADRFPVFYRVGTGTQYVKDTNGKITYLMGAYSYKTGGIVNTPA
                     ISDWSFVTNTTNTPLSTYGTPGDSGSPLFAWDADQNKWVLLAVLNSYAGVNGNTNWYT
                     IIPAGDVKNTMKQDADAPVNTKQGEGDIHWSYDEKTGLGSLTQGSTSWEMHGNLGATW
                     PASLNSGKDLTFQGGGTVVLENTVNQGAGTLTFDDDYIVKPVDTQTWKGGGIIVNGEH
                     LVDWQINGVTGDSLHKLGTGTLKINGTGVNPGSLSVGDGTVILAQRADDNGLSQAFSS
                     VSIVSGRPTLVLNDDKQINPDNIKWGYHGGKLDINGNSLTFHKLNGADDGAILTNSGS
                     MANVNLDFNSPDTTATIANIWHGHFTGNLNINNEVTAGTQNDFAIDGGVNAQGSITQQ
                     NGRLFMQGHPVVHAVSSQDVANKLKALGDNSVLTQPVSFTQNDWENRQFSMAELNLQN
                     AEFNLARNASLNTRINAEHSTVTLGSEDLYIDLNDGNGVATKPTLGKSKATAEDDQSR
                     FNGHVQLQQGSALTINEHFAGGIDSADSATTITSTDTTLNQLSRFTQSSLSLGEGAKL
                     TATAGLLSDGTVSSNAGASLSLLSDQPGTMYSAQSWELSGQDTSLNVGAGGIITGDIN
                     ANDAASISFGTTDINQSTNYYGNINAPLASVTMKDTAWQVNKQSVAKSLTLNGSTLSF
                     NRFGQGGLTSDTLEATNSSFIINADGKAADTVTVNQALTGGNNTLVVIPTTNSVKQGG
                     DPVSLVTAPKNTQSNIFTLNPVSINAGFHSFTPQLDVLETDVNKQWRLEGFYIQPDKA
                     ALRTGKSFMDLGYKNFITEINNLNDRMGDLRHTHGETGAWARLNSGSGSATDGFTGSY
                     THLQIGADRKHIIEGGELFTGVTATFTSSNNRGTGWSGRTKSTGIGVYASAMFDSGLY
                     VDTIGKYVRHDNHYSSSALGMPEQDYGSHSWYLGAEAGWRFSLPDETYIQPQTELIYG
                     TVSENQFAWQFNGGEIYMQRKQMQPLIGRTGIEFGKTFRGKDWEMTALTGINYQYDLF
                     KPTVT"
BASE COUNT         1076 a          738 c          882 g          942 t
ORIGIN      
        1 aatataggtg atatgaaaat tacagtcctt aatcagcgat ataaaaaaca ttttggttat
       61 cctcatattc tgatgggtga tgtaaaagta actgtttcag caatgattgc ttgcgctgtt
      121 ttattgcacc caaataaatt gtttgcctcc gtcgctggaa cctcacttcc ctatcaaacg
      181 tatcgtgatt ttgctgagaa taagggagag ttccgtcctg gcgcagaaaa tgttcctcta
      241 tatgataaaa atggcaatcc cgtcacgacg ctgaataagg cacccatgat tgacttcagc
      301 tctaatgact cgactggggt tgcaacactg gtttcgccac agtatgtcgt cagcgttaaa
      361 cacaatgttg gttatcaata tgtgaaattt ggctatgcag atgattcatc ctatgccctt
      421 gttgacagaa ataaccactg gagagatttc cacactcccc gtctcaacaa aattgtcacc
      481 gaggttacac ctctggatat aaccaatgcg ggtacagcta agggaaccta tcaaaatgct
      541 gaccgtttcc ctgtgttcta tcgagtaggt acgggaactc aatatgtaaa agatactaac
      601 ggaaaaatca cctatttaat gggagcctac tcgtataaaa caggtggaat agtaaatacg
      661 ccggctatat ctgactggag ttttgtcacg aataccacta atactccact ctcaacctac
      721 ggcactcccg gtgacagtgg ctctcctctt tttgcctggg atgcagacca aaataaatgg
      781 gttttgttag ccgttctcaa ttcatatgca ggggtcaatg gcaatactaa ctggtacaca
      841 ataattcctg ctggtgatgt aaaaaatacg atgaaacaag atgccgatgc accagtaaat
      901 acaaaacaag gtgaagggga tattcattgg tcatatgacg aaaaaacggg acttggttca
      961 ttaacccagg gaagcacatc atgggaaatg catggaaatt tgggggcaac atggcctgct
     1021 tcgcttaata gtggtaaaga tctaactttt cagggcggcg gtactgtagt gttagaaaat
     1081 accgttaatc agggggctgg cacacttact tttgatgatg attatattgt caagcccgtt
     1141 gatactcaga catggaaagg cggcggaatt attgttaatg gtgaacatct tgttgactgg
     1201 cagattaatg gggttactgg tgatagtttg cataaactgg gtacgggtac gttaaaaatt
     1261 aacggtaccg gtgtaaaccc aggaagctta agtgtgggag acggaaccgt tatattggca
     1321 cagcgtgctg atgataacgg actgagccag gcgttcagtt ctgtcagtat tgtaagtggg
     1381 cgtcctactc tggtgctaaa cgacgacaaa caaattaatc cggataatat caaatggggc
     1441 tatcatggcg gcaagctcga cattaatgga aacagtttga cttttcataa acttaacggg
     1501 gctgatgatg gagctatact gactaacagt ggctccatgg ctaatgttaa tctggatttc
     1561 aacagcccag atacaacagc cacgattgct aatatctggc acggccattt caccggtaat
     1621 ctaaatatca ataatgaggt taccgcagga acacagaatg atttcgctat tgatggtgga
     1681 gtgaatgctc aggggagtat aacccagcaa aatggtcggc tatttatgca aggacatccg
     1741 gttgttcacg ccgtcagcag tcaggacgta gcaaataagc taaaggcgct gggtgacaat
     1801 tcagtactga cccaaccagt gtcatttact cagaacgatt gggaaaatcg acagttcagt
     1861 atggcggagc ttaacttaca aaatgctgaa tttaaccttg caagaaacgc cagccttaat
     1921 accagaatta atgccgagca ttctaccgtg acgttaggta gtgaggatct ttacattgat
     1981 ctcaatgacg ggaatggcgt ggcaaccaaa ccaacattgg gtaagtcaaa ggcaaccgct
     2041 gaagatgatc agagtcgttt caatggacat gtacagttgc aacaaggatc ggcactaact
     2101 attaatgaac attttgccgg cggtattgat agtgcagata gtgcgacgac gataacctct
     2161 acagatacca cgcttaatca gctcagccgt ttcacccaat cttctttgtc attaggtgaa
     2221 ggggcaaaat tgaccgctac agccggatta ctttctgacg ggacggtaag cagtaatgcc
     2281 ggggcgagcc tttccttgct atcagaccag cctggaacga tgtattccgc ccaaagctgg
     2341 gagcttagcg ggcaagacac gagcttaaat gttggcgcgg gtgggatcat cactggagat
     2401 atcaatgcca atgatgcggc cagcattagc tttggcacga ctgatattaa ccaatccaca
     2461 aattactatg gaaatatcaa cgcgccgttg gcaagtgtaa caatgaagga cactgcctgg
     2521 caggtaaaca agcagtcggt ggctaagtcg ctcactttga atggttcaac actgagcttt
     2581 aatcgtttcg ggcaaggggg actgacgtca gatacgctgg aggccactaa cagttcgttc
     2641 attatcaatg ccgatggtaa agcagcagat acggtgacgg taaaccaggc attgacaggg
     2701 gggaataaca cactggttgt tattcctacg acaaattcag taaaacaggg cggtgaccct
     2761 gtgtcattgg ttactgcccc gaaaaataca caaagcaata tatttacact taacccggtt
     2821 tcaataaatg cagggttcca tagttttaca ccgcaacttg atgtgttgga gaccgacgtt
     2881 aataagcaat ggcgtctgga gggattttac atacagcctg ataaagccgc tttgcggaca
     2941 ggcaagagtt ttatggatct cggttataaa aactttatta cagaaattaa taatctgaat
     3001 gatcgtatgg gggacttacg ccatacccat ggtgaaactg gggcatgggc tagacttaac
     3061 agtggttcgg gctcagctac tgatggattt accggaagtt atacgcactt gcaaattggg
     3121 gcagacagga aacatataat tgaaggcgga gagttattta ctggagtaac ggcgacattt
     3181 acaagcagta ataaccgggg aactggttgg tcgggaagaa ccaaatcaac gggtattggt
     3241 gtgtacgctt cagctatgtt tgactctgga ctctatgtag atactatcgg gaaatatgtg
     3301 cgtcatgaca atcattactc atcaagtgcg ttaggaatgc cagaacaaga ttatggctcg
     3361 cactcctggt atttgggcgc tgaggccggt tggcgttttt ccttacctga cgaaacatat
     3421 atacagccgc aaacagagtt gatctacggt actgtatctg agaatcagtt tgcctggcaa
     3481 ttcaatgggg gtgagatcta tatgcaacgt aagcagatgc aaccattgat tggtcgcaca
     3541 ggaattgaat ttggcaaaac attccgcggc aaagactggg aaatgacagc gctgactggg
     3601 attaattatc agtacgattt atttaagcct actgtgac
//
LOCUS       DACADF010000267         3598 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.270, whole genome shotgun sequence.
ACCESSION   DACADF010000267 DACADF010000000
VERSION     DACADF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.270"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            87..608
                     /locus_tag="ISB30_004720"
     CDS             87..608
                     /locus_tag="ISB30_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HAP1992262.1"
                     /translation="MGDLLDKLCMLDSDGFIYGQNLRLCTEARYICPVQQIIDTPLLR
                     KLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGSLLAED
                     MNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDEDFSLKNSRDGNPRKWRFD
                     ATNSELCAERHEC"
     gene            741..2003
                     /locus_tag="ISB30_004721"
     CDS             741..2003
                     /locus_tag="ISB30_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAP1992263.1"
                     /translation="MKANFTLSDGDKAVTDADGKAKVTLKGTKAGAHTVTASMVGGKS
                     EQLVVNFTADTLTAQVNLNVTEDNFIANNIGMTRLQATVTDGNGNPVEGIKVNFRGTS
                     VTLSSTSVETDDQVFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKVDVNSATIT
                     SQEIPEGQVMVAQDIAVKAHVNDQFGNPVTHQPATFSAAPSSQMIISQNTVSTNTQGV
                     AEVTMTSERNGSYTVKASLANGASLEKQLEAIDEKLTLTSSPLIGVNAPKGATLTATL
                     TSANGTPVEGQVINFSVTLEGATLSGGKVRTNSSDQAPVVLTSNKVGTYTVTASFHNG
                     VTIQTQTTVKVTGNPSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVY
                     FALKSGSTTLTSLTAVTDQNGIATTNVR"
     gene            2223..2699
                     /locus_tag="ISB30_004722"
     CDS             2223..2699
                     /locus_tag="ISB30_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HAP1992264.1"
                     /translation="MQSGTNVPYMKISAIDYSQNINGDYKATVTGGGEGIATLIPVLN
                     GVHQAGLSTTIEFISAETRPMTGTVSVNSANLPTASFPSQGFTGAYYQLNNDNFAPGK
                     TAADYSFSSSASWVGVDATGKVTFKNDGDSNTVIITAPPRSGGAIYQTVPPESRSV"
     gene            complement(2827..>3598)
                     /locus_tag="ISB30_004723"
     CDS             complement(2827..>3598)
                     /locus_tag="ISB30_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HAP1992265.1"
                     /translation="KEIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNF
                     QVVGLTMKCYSPLCKNASRMDQNLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLD
                     EVREEMRSLGSYIALNLEGSSQERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKA
                     RALVDCYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYK
                     TRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1041 a          841 c          822 g          894 t
ORIGIN      
        1 acatcctact atcgatctat ttccagtctc ctgtgatact taagaagata caaactttta
       61 atggaaaaat aaaattcagg cagtttgtgg gagacctact tgataaacta tgcatgcttg
      121 acagtgacgg ttttatttat ggtcaaaatt taagactctg tacagaagcc agatatattt
      181 gtcctgtgca acaaattata gatacccccc tgttacgcaa actcaaccag tttcgtacgt
      241 ttgttcgtaa tgtccgcccg ggtgatgaac tggatgtcca ggcacaagtt agtgaaaaaa
      301 atttaacccc gccgccgggt aacagcagtg gcaaccttga gcaacagata gccagtactt
      361 cacagctaat tggatctctg ctcgccgaag atatgaacag cgagcaagcg gcaaacatag
      421 cacgtggatg ggcctcttct caggcttcag gggtaatgac agactggtta agccgcttcg
      481 gtaccgcaag aatcacgctg ggcgtggatg aagatttcag cctgaagaac tcccgtgacg
      541 gcaaccctcg aaaatggcga ttcgatgcaa caaacagtga actatgtgcc gaacgtcacg
      601 aatgctgaaa tcacgctggc agcctcgaag gatccggtga ttgccgacaa taacgatctc
      661 acgacactaa cagcaccgtc gctgatacag agggcaatgc gatagccaac actgaggtaa
      721 catttactct accggaagat gtgaaggcga acttcacgct gagcgatggc gacaaagcgg
      781 ttactgatgc cgatggcaaa gcgaaagtca cgctgaaagg tacaaaagca ggcgctcata
      841 ccgtgacagc atcgatggtt ggcggtaaga gtgagcagtt ggtggtgaac tttactgcgg
      901 atacgctcac tgcgcaggtt aatcttaacg ttaccgagga caatttcatc gccaataaca
      961 ttggaatgac caggctacaa gcaacagtga ctgatggaaa cggcaaccca gtagaaggta
     1021 taaaagttaa tttccgtgga acctccgtca cgctaagcag caccagcgtt gaaacggatg
     1081 atcaggtttt tgctgaaatt cttgtgacaa gcaccgaggt cggactaaaa acagtttcag
     1141 cctctctggc agataaacct actgaagtca tctcgcgatt actgaatgcc aaggtagatg
     1201 ttaattctgc gacgattacc agtcaggaga tacctgaagg tcaggtcatg gtcgcacaag
     1261 acatagcagt taaagctcac gttaacgacc agtttggcaa cccggtgacg catcaacccg
     1321 cgacattcag tgcagcgcca tcctcgcaaa tgatcatcag ccagaacacg gtctctacta
     1381 atacgcaggg agtagccgag gtcaccatga cgtctgaaag aaacggctcg tatactgtaa
     1441 aagcatccct agcgaatgga gcttcacttg agaaacaact ggaggctatt gatgaaaaac
     1501 tgacactcac gtccagtccg cttatcggtg tcaatgctcc taaaggcgct actctgacgg
     1561 caacgctaac ctctgcaaat ggcactccag tggagggtca ggtcatcaac tttagcgtaa
     1621 cgctagaagg tgcgacgtta agtggcggaa aagtgagaac caactcttca gatcaggctc
     1681 cagtcgttct gaccagcaat aaagtcggta catatacggt gactgcatcg ttccataacg
     1741 gcgtcacaat acagacacag acaaccgtga aggttactgg caacccaagc accgcacatg
     1801 ttgctagctt tatcgctgat ccgtcgacta tcgccgccac caacagtgat ttaagtacct
     1861 taaaggcaac ggttgaggat ggcagtggta acctgatcga aggtctcact gtgtacttcg
     1921 ccttaaaaag cggctccacc acattaacgt cattaacagc cgtgaccgat caaaacggaa
     1981 tcgcgacaac aaacgtgaga tgagcgataa cagggagcgt cactgtaagc gcagtcataa
     2041 gcgcaggtgg aatgcaaaca gtagatatat cgctagtggc aggcccagca gacgcctcgc
     2101 agtccatcct taagaacaac cagtcatcac tgaaagggga ctttaccgat agtgctgagc
     2161 tacatcttgt tctgcacgat atatcaggca atccgatcaa agtttcttaa gggatagaat
     2221 ttgtgcaatc aggtacaaac gtgccctata tgaaaattag cgcaattgat tacagtcaaa
     2281 atatcaacgg tgattacaaa gctactgtta caggaggcgg agagggtatc gcaacgctga
     2341 tccccgtatt gaatggtgtt catcaggcgg gtctgagtac cacgatagaa ttcattagcg
     2401 cggaaaccag accaatgacg ggtacagtgt cagtgaatag tgcaaaccta ccgacagctt
     2461 cattcccttc gcagggattt accggggcgt attatcagtt gaataatgac aactttgcac
     2521 caggaaaaac ggcggctgat tattcgtttt caagctcggc ctcctgggta ggcgttgatg
     2581 ctactggtaa agtgacattc aaaaatgacg gtgatagcaa tactgtaata atcacagcgc
     2641 ccccaagatc cggtggtgct atttaccaaa cagttcctcc tgaatcgcgt tcggtgtgac
     2701 tatgatcatt ttcggtgagt attgctttga tccaaaggct ggttttctgt gatgattgac
     2761 cagcctcaga gtattgatga caccaccgtt cataacagat cggcattact atgcataagt
     2821 actgcctcaa attctcgcca aaacactttt taatgccttt gcgaattcat ccagacttat
     2881 attgtcaatt ttttgcccaa cgacgactga ctccgaaaca tcggccattg cgggccatcg
     2941 cgttttgtaa tcagcataaa ttgcaacaac aggggtatta taggcacttg ccatatgtaa
     3001 tattgaggtg tcaggagtta ttgcaatata agcatctttt atgattgctg ctgaacgttt
     3061 aatcgagggt gataaggata aacggtagac attattataa caatccacta atgccctggc
     3121 tttatcttcc cctttgggtc cataaacgat cactattgga atatctgttt cactttgaat
     3181 ttttgctatt agattttctg caatcgataa tgaaaatgta cgttcctgcg agctaccttc
     3241 taaattgagc gcaatgtaag atcctaacga tcgcatttcc tcgcgtacct catcgagtac
     3301 atcatcgctt aatggcaatt catatattga cctgactgcc ggaaaacccg cctcacgcat
     3361 cataaatgcc catgtcatgg gtacaggagc ccggagattc tgatccatcc gggacgcgtt
     3421 cttacacaaa ggggaatagc acttcatggt taaaccgacg acctggaaat tcgtttttgc
     3481 tttcaactga ctgataaata tcatcgtttt caggttcttt ttacgcatcg cctctataca
     3541 aagatccggc gtaccatatt gcagtgttat gttcttcgct aaatctttta tttctttt
//
LOCUS       DACADF010000268         3579 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.271, whole genome shotgun sequence.
ACCESSION   DACADF010000268 DACADF010000000
VERSION     DACADF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.271"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            228..515
                     /locus_tag="ISB30_004724"
     CDS             228..515
                     /locus_tag="ISB30_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992266.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            634..1455
                     /locus_tag="ISB30_004725"
     CDS             634..1455
                     /locus_tag="ISB30_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAP1992267.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(1752..2354)
                     /locus_tag="ISB30_004726"
     CDS             complement(1752..2354)
                     /locus_tag="ISB30_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP1992268.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWQESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVTWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRLPATKKSLPEINSVQNN"
     gene            2685..3068
                     /gene="traM"
                     /locus_tag="ISB30_004727"
     CDS             2685..3068
                     /gene="traM"
                     /locus_tag="ISB30_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HAP1992269.1"
                     /translation="MAKVQAYVSDEIVYKINKIVERRRAEGAKSTDVSFSSISTMLLE
                     LGLRVYETQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPENDEG"
     gene            3202..>3579
                     /locus_tag="ISB30_004728"
     CDS             3202..>3579
                     /locus_tag="ISB30_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAP1992270.1"
                     /translation="MRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKKRKILYANGA
                     FIELFSKEDKPFSGESYVRLQVEIFLSSLELECQSLGHGSAFCRRFNFHGEIYQIRME
                     NVSFYNEESVVLWQINIFPDYPFF"
BASE COUNT         1044 a          691 c          802 g         1042 t
ORIGIN      
        1 acatcccgca aaactttttg cccagaagca ggcaaaaact tttgcggtct gcccccggac
       61 agtcgtttcc gtccgtggca gtgatgcccc ttgcggcgaa ggttctcaaa aaggtgatgg
      121 tgaataccgg agggaacaaa aggcaggtcc ccgcaaaacg tcaaaaccgc ggctcccgaa
      181 actcaacaga tgatgggggc tgaaaaccag agagaggaga aagcaggatg agtacccgaa
      241 atatccacgt taacaccgcg tcgtacacgc ttcttgttgc cgggaagaaa aaaaacaccg
      301 gggaagagtg ggacgtcctg gaattcagca gcctgactga gctgaaaaaa tatcgcaaaa
      361 gccacccgga aaagatggcc ttcagttata gctacgcgct cagtcagggt gtggataagc
      421 agttccgcca tatcaacatt gcggaagccg atcacttcaa acagttcctg cgtcagatta
      481 agcgtgccgg cctcgatatc cgggcgatct gctgaccgga tgtgatgaat acccctcctg
      541 attgttgcaa tcttgttaat ggagatcggg ggggatgata ttaagcggaa aaatttttta
      601 ttaaatttat tctttcataa aggagtagct gttatgcgat tagcttcccg ttttggtcgg
      661 tataattcca tccgccgtga acgtccttta acggatgatg aattaatgca gttcgtgcct
      721 tcggtatttt ccggtgataa acatgagtcc cggagtgaac gttatacgta tattccaaca
      781 atcaatatca tcaataagtt acgtgatgaa ggtttccagc cattctttgc ctgtcagagt
      841 cgggttcgtg atttgggacg tcgcgaatac agtaaacata tgttacgtct tcgcagggaa
      901 gggcatatta acggacagga agttcctgaa attatcctgc ttaattcaca tgatggttca
      961 tccagttatc agatgatccc cggaattttt cgttttgtct gcacaaatgg cctggtgtgc
     1021 ggaaataatt ttggcgaaat ccgcgttcca cataaaggtg atattgtcgg gcaggttatc
     1081 gagggagcgt atgaagtgct cggtgtcttt gataaggtca ctgataatat ggaggcgatg
     1141 aaagaaattc atcttaacag tgacgagcaa catttatttg gcagagctgc actgatggtc
     1201 aggtatgaag atgaaaataa aacgccagtg acacctgaac agataattac tccccgtcgt
     1261 cgggaagaca aacagaacga tctctggaca acctggcagc gggttcagga gaatatgata
     1321 aaaggtggat tatcggggcg aagtgcctcc gggaaaaata ccaggacaag agccattaca
     1381 ggtattgatg gtgatataag aatcaacaag gcgttatggg tgattgccga acagttcaga
     1441 aagtggaagt catgataata taagtaaagc cccgaaaaaa ttttcggggc tttacttata
     1501 ttatcatttt tcggcattgc tgtgtcgtct gatatacctc aatttcatca accggctttc
     1561 cggttgtctg taaatattgt gcctgcgaaa aatggtctgg caggaggctg cgcagttacc
     1621 gggtatatat aagatgggtt cccgcttcgc ggggcttcgg ccagtcagta ttgtcactca
     1681 tatgatattt ttgtgtgtgg ccttccatgc cgcttttgcg gcataaccgg tatctgacaa
     1741 tgtctgtaag attaattgtt ctgcacgctg ttaatttccg gaagtgattt cttcgtggcc
     1801 ggaagccgga tacctttact gctttttatc ccggtataaa tccggtaaac atctgatgcg
     1861 taggcaagac gtctctggtt ctggcgttcg gttttcctga atccggcatt gtatgcacca
     1921 acggcctccc aggtgacgcc ccatttttta aatgctattg ccagataata agcaccggta
     1981 taaatgttca tgcagggatc tgttgtcaga tgttctggct taattccgta gcgggccagt
     2041 tcgttaaaat gctgggaatc tacctgcatc agtccgctgc catatcccgt taccggatta
     2101 ataccgatgg cattaacccg gtaacgggat tcttgccatg atattgctct cagtaaatca
     2161 ggatctattt tgtaatcccg gcctgcaaga tcaaagcaat cagtggcttg gcagatctca
     2221 tttataaaca tcaggcagat ggctaacatc cattttttca tttttccacc tctggtgact
     2281 ttatccgtaa ataatttaac ccactccaca aaaaggctca acaggttggt ggttctcacc
     2341 accaaaagca ccacacacta cgcaaaaaca agtttttgct gatttgctac ttgaatcatt
     2401 aacttatgtt ttaaataatg tattttaatt tattttacat tataaaaagg attcattggt
     2461 gaatcgcata tgattcacca atgaatttaa cacaactcat agatgaatca gtaataataa
     2521 aacaacgagt taaagcgaat tgactctaga ttcaattcgt gatgtgattt gctgcatgaa
     2581 tcgtaatttt cgtaacttgc gtcacgattc gattcatcga tgaattgtct tttttttata
     2641 tgtgaaataa atttgatgcg ataagaaaat aaagaggtat acatatggcg aaagtgcagg
     2701 cttatgtcag tgacgaaatt gtctataaaa taaataaaat tgtggaaaga agacgtgcag
     2761 aaggagctaa aagtacagat gtaagtttct caagcatatc cactatgctt ctcgaattag
     2821 ggcttcgtgt atatgagact cagatggagc gtaaagagtc tgcatttaat cagactgagt
     2881 ttaataaatt gcttcttgaa tgcgttgtaa aaacacaatc atcagtagcg aaaattttgg
     2941 gtattgagtc tctcagtcct catgtctccg gaaatccaaa gtttgaatat gcgaatatgg
     3001 tcgaagatat cagggagaag gtgtcatctg agatggagag gttctttcca gaaaatgatg
     3061 agggttaaaa aagaattaaa agttgatttg cttcaaataa caggattttg atctggttca
     3121 atttgatgac aattaaccgc ataaaggtta tattaattat gtggttaatg ccacgttaaa
     3181 acttgatatt aaaaatcgcc gatgagagaa aggccactta acagtcaatc tgtaaataaa
     3241 tacattctta acgttcaaaa tatctacagg aattctcctg ttcctgtttg tgtccgtaac
     3301 aaaaaaagga aaatccttta tgccaatggg gcctttattg aactcttttc caaagaagat
     3361 aaacccttct ctggtgaaag ttatgtacgt ctgcaggttg aaatttttct ttcatcactt
     3421 gagcttgaat gccagtctct tgggcatggt tccgcatttt gtcgtcgttt taattttcat
     3481 ggagaaatct atcaaataag gatggaaaat gtttcatttt ataatgaaga atctgttgtt
     3541 ctatggcaaa ttaatatatt tcctgactat ccattcttt
//
LOCUS       DACADF010000269         3469 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.272, whole genome shotgun sequence.
ACCESSION   DACADF010000269 DACADF010000000
VERSION     DACADF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.272"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            246..482
                     /locus_tag="ISB30_004729"
     CDS             246..482
                     /locus_tag="ISB30_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP1992271.1"
                     /translation="MVKLALQPRDSVACIAREHDINDNLLFKWLRLWLIKGRISRRLP
                     VTNSSGIGVELLPVEMTLDERPYPVFTRGNYEDQ"
     gene            complement(682..1557)
                     /locus_tag="ISB30_004730"
                     /pseudo
     CDS             complement(682..1557)
                     /locus_tag="ISB30_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            complement(1836..2086)
                     /locus_tag="ISB30_004731"
                     /pseudo
     CDS             complement(1836..2086)
                     /locus_tag="ISB30_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2174..3418)
                     /locus_tag="ISB30_004732"
     CDS             complement(2174..3418)
                     /locus_tag="ISB30_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1992272.1"
                     /translation="MLQIKARTKILALFCAMSFLMYVDRVNLSATAGLIKDELGLSNT
                     ELGVIFSAFAYTYAVFQIIGGWFVDRLGSRRMIIFCGIIWVIATMATGFVGGFISLFL
                     ARLLLGVGEGAALPSQARAITYWFKPQQRGFVQGVTHSFSRLGNAITPPIIVALVAFW
                     SWRGAFISLAIVTFIWLISWIISYKDDPRDHSGMTEEELEGVPIFEKKDLKVKAEPTP
                     WRALIKRMGPTMGVYFCYGWTSWLFFTWLPTFFMHGRGMDLKSSALFTAGVFLSGVVG
                     NTVGGVISDKVLKMTGNLVAARRNVILFSFISVLFLLVPVINTDSLLIMTICLSVAFF
                     CLELTIGPIWAVPMDITPKYVGIASGLMNAGSAVAGIISPIVFGIIIDKTGNWSLPFY
                     GSVALLIIGIFLTFFMRPDKSL"
BASE COUNT         1044 a          828 c          744 g          853 t
ORIGIN      
        1 tggtcacaat ttcagatttt ttccagtatc tattttccga ttcgttcggc ggtaatccac
       61 cgttatattc atgaggccgg agtgcactat aaaagtgtat gcgtaaaccg actgctgtat
      121 gtagccatta agcctgtatt ggtaacatag gtacccacct tttcaacaag tggacacctg
      181 ttatagaaca aaaaacatta tcagcagaac cccgcaggtc attttcaaat gagtttaaac
      241 ttcaaatggt taaactggct ttacaaccaa gggattctgt tgcctgtatt gcccgggagc
      301 acgatatcaa tgataatctt ctgttcaaat ggctcaggct ctggctgatc aaagggcgta
      361 tatcacggcg tcttccggta acgaactctt ctggcattgg cgttgaatta ttacctgtgg
      421 agatgacgct ggatgagcgg ccttaccccg tttttacgcg aggtaattat gaagatcaat
      481 gactagccgt cgaaccgtgt gcatgaactg ctgtactgaa acttctcagc tgtaaaataa
      541 tccttacgct acgtacttct cggagcgctt acgattaatc agatgcagtc ctaactggaa
      601 gaaagaaaaa tggagaaaat aagaggaatt aatatcctcc acgttcctga aatcctggag
      661 gatattaatg tcatcaaaat attagcgaac gataatccct aacaaaatac ccagtgcgcc
      721 aaagtcagca tcactgaagg tggtgttagc aaaaccaata tcacccagca caggcaataa
      781 gaatactgac aggaaggtaa taagcaagcc ttgcgcaaag gcacccaaaa tcgcaccacg
      841 acgtccaccc gttgcattac cgaatacccc tgcagctgca cctacgaaga agtgcggcac
      901 cacaccagga ataatcacga tcatgttcag cagatacaac gtgaacatac cgattaaacc
      961 tgcagcaaag ctactcagga aaccaaccag tacagcgtta ggtgcgtaag ggaagatgac
     1021 cgggcaatcg agagcaggtc tggcattcgg aaccagcttg tctgaaatac ctttgaatgc
     1081 cgggacaatc tccgcaatca ccatgcgcac accctgcaga atgatgtaca cgccagctgc
     1141 gaaggtaatg gattgcatga tagagaacat gaaccagttt ttaccaccgc taagctcttt
     1201 caccgcatct gcacccgcaa acaggcacgt cagcaggaaa attatgatca tggtaaaaga
     1261 gatagcaacc ggcgtgtcgc gcaagaacag cagacttttc ggcacattca tgtcttcagt
     1321 cgagtgctct ttattaccaa acttgctgcc gatgaaaccg gccaatacat aagataatgt
     1381 tgagaagtgg ccgatggcaa cgtcatcaga accggtcact ttcttcatgt acggatgagc
     1441 aatagccggg aaaaagacca ttgccacgcc aacaaccaaa gagccaacgg ctataagcgt
     1501 aatgccggtc atgcctgctg ttgccagaat taccgctacc atcatcgaca taaacaacgc
     1561 ccgcagcatg agaatggaca atagacattc gggaggattt tttcatataa atctccttgc
     1621 tcatttttaa tgaacgaagg aggagacgac ggagaaatca ccttcctgaa cgggatcaag
     1681 ctaccactgt cggatccgcc acactccctg aaccatgttt tttaagggtc ataccaccaa
     1741 aaggtcgacg gctgcttcct tcgctatatt agactgccag ttttaccggg gagtttctga
     1801 gactcgggac ggggtttcac acatggaaag ttttttaata tttcgttttc catttcaatt
     1861 cgttgtagct ttttctttag ctcacgtagt ttgatttgtt ccggtgttat cagggaggct
     1921 tttggcgttt accctgacgc tcatcacgca gttgtttgaa ccattccgtc atcgtggaaa
     1981 gaccaacatc catagcctta gcagcgtcag aaacagtgta attttgatcg acaaccaact
     2041 gagcggattc acgtttgaat tcaggactga aatttcttct tttcataggt gcacctgtgt
     2101 tgttctaagg tgagcatatc accctgttca gatggccaaa ttcagtagac cacttcagcc
     2161 ctgaattcga cttttacaat gacttatctg gacgcatgaa gaacgtcaga aatataccaa
     2221 taataagcaa tgcgacggaa ccgtagaatg gtaaactcca gttgcctgtt ttgtcgataa
     2281 tgatgccaaa gacgataggt gaaataatgc cggcgacagc ggaaccggcg ttcatcaatc
     2341 cgctggcaat tcccacatac ttcggcgtaa tatccatcgg gacggcccag ataggcccta
     2401 tggtcaactc aagacagaag aacgccacac tcagacatat agtcataatg agcaacgagt
     2461 cagtatttat aaccgggacg agcagaaata gtacggagat aaagctgaaa agaatgacgt
     2521 tacgccgggc tgctactaga ttgccagtca tcttcagcac tttgtcgctg attacgccac
     2581 caaccgtatt acctactaca ccggacagaa atacgccggc agtaaagagc gccgatgact
     2641 taagatccat gccgcgacca tgcataaaga aagttggtaa ccaagtaaaa aacaaccaac
     2701 ttgtccatcc gtaacagaaa taaactccca ttgtcggtcc cattcgtttt atcagggctc
     2761 tccatggagt aggctcagct ttaactttta gatctttttt ctcgaaaatt ggtacgcctt
     2821 ctagttcttc ttcggtcata ccgctgtggt ctcgtgggtc atctttatac gatatgatcc
     2881 aagatattaa ccagataaat gtgacaatag ccaaactgat aaatgcgcca cgccaagacc
     2941 aaaatgccac taatgcaaca ataatggggg gagtaatcgc atttcccaaa cgagagaatg
     3001 agtgcgtaac gccctgaaca aagccacgct gctgcggctt gaaccagtag gtgatcgctc
     3061 gcgcctgaga aggcagcgcc gcaccttccc ctacacctaa tagcaatctg gcgaggaata
     3121 gtgatataaa gccaccgacg aaaccagtcg ccatggttgc tataacccaa ataatgccac
     3181 aaaaaatgat catgcggcgt gagccaagtc gatcaacgaa ccagccaccg atgatttgaa
     3241 aaactgcata ggtataagca aaagcagaaa aaataacgcc gagttctgta ttactcagtc
     3301 cgagttcatc tttaatcaac cccgcagtag ctgataagtt tactcggtcc acatacatta
     3361 ggaatgacat tgcgcaaaat agcgcaagta tcttggttct ggcttttatt tgtaacattt
     3421 ttgtctccta aactagcgtt aaattgctag taaataatgt caattagtt
//
LOCUS       DACADF010000270         3376 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.273, whole genome shotgun sequence.
ACCESSION   DACADF010000270 DACADF010000000
VERSION     DACADF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.273"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            96..812
                     /gene="nanC"
                     /locus_tag="ISB30_004733"
     CDS             96..812
                     /gene="nanC"
                     /locus_tag="ISB30_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAP1992273.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFVWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            832..1938
                     /locus_tag="ISB30_004734"
     CDS             832..1938
                     /locus_tag="ISB30_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAP1992274.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGDLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNRLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGESAGGKAVTDSVLISVKDNKVTVQN"
     gene            2002..2982
                     /locus_tag="ISB30_004735"
     CDS             2002..2982
                     /locus_tag="ISB30_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAP1992275.1"
                     /translation="MNAIISPNYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFTHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQFHSQLNNIT
                     AAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYFGSAYRSAENWTTSLRSSHFSAAARRGIISDRFVEAILQFWRER"
BASE COUNT          985 a          724 c          794 g          873 t
ORIGIN      
        1 actataagcc tggcaaaaaa tattacacaa cataaatgct aattgtttat gcgggctttg
       61 tattgctttc tgtatcctac aaatgagtga aatttatgaa aaaggctaaa atactttctg
      121 gcgtattatt actgtgcttt tcgtccccat taatttctca ggctgcgaca ctggacgtac
      181 gtggtggata tcgtagtgga agccacgcct atgagactcg actcaaagtc agtgagggat
      241 ggcaaaatgg atggtgggca agcatggaaa gtaatacctg gaataccatt catgataata
      301 aaaaggaaaa tgccgcactc aatgatgttc aggttgaagt taattacgca attaaacttg
      361 atgatcaatg gacggtacgc ccgggaatgt taacgcattt tagcagcaac ggcacacgtt
      421 acggacccta cgtaaaactg tcctgggacg cgacaaaaga tcttaatttt ggcattcgct
      481 atcgttacga ctggaaagct taccgacaac aagacttatc cggtgatatg tctcgtgata
      541 acgttcatcg ttgggatgga tatgtcactt accatattaa tagtgatttc accttcgtat
      601 ggcaaacgac gctatacagc aaacagaacg attatcgcta tgcaaaccat aagaaatggg
      661 cgacggaaaa tgcatttgtt ctacaatacc atatgacgcc cgatattacg ccatacatag
      721 aatatgacta ccttgaccgt cagggtgttt acaacggcag agataattta tcggaaaaca
      781 gttatcgcat tggtgtgtca tttaaactgt agtagacagg agacagtcac aatgaataaa
      841 acaataacgg cgcttgctat catgatggct tcatttgccg caaacgcgtc tgtattaccg
      901 gaaactcctg tgccatttaa aagtggtacc ggagcaattg ataacgacac tgtctacatt
      961 ggtttaggta gcgcaggtac ggcatggtac aagctggata cacaggccaa agataaaaaa
     1021 tggacagcgt tagctgcatt ccctggtgga ccaagagatc aagcaacctc ggcatttatt
     1081 gatggtgatc tgtatgtgtt tggcggcatt ggcaaaaata gcaagggctt gactcaggta
     1141 tttaatgacg tacacaaata caaccccaaa accaatagct gggttaaatt gatgtcgcac
     1201 gcgccaatgg gcatggcggg ccatgtgact tttgtacaca acggcaaagc ttatgttact
     1261 ggcggtgtta accagaatat cttcaatggc tattttgaag atctcaacga ggctggaaaa
     1321 gattcaaccg ctatagataa aatcaacgct cactattttg acaaaaaagc agaagattat
     1381 ttcttcaata agtttctgtt gtcttttgat ccctcaaccc agcaatggag ttacgctggc
     1441 gaatctccct ggtacggaac ggctggtgcg gcggttgtga ataaaggtga taaaacctgg
     1501 cttattaatg gcgaagccaa acccggattg cgaacggatg ccgtatttga acttgatttc
     1561 accggtgata atttaaaatg gaataggctt gctcccgtct catcaccaga tggcgtcgct
     1621 ggcggttttg cggggataag caatgattct cttatatttg ccggaggggc cggattcaaa
     1681 ggttcacgag aaaattacca gaacggtaag aactatgcgc atgaaggcct gaaaaaatca
     1741 tatagcactg atattcatct ttggcataac gggaaatggg ataaatcggg tgaattatcg
     1801 caaggtcggg cctacggagt atcattgccc tggaataata gtctattgat tattggcggt
     1861 gaatctgcag gcggcaaagc ggtgacggat tcagttttga tctctgtgaa ggataataaa
     1921 gtcacagtac aaaactaacg cttcagggcc ccggtaaagg gccctgctat caatttgtca
     1981 tttcaattac gcgaaaatta tatgaacgca ataatatcgc ccaattatta ctatgttctt
     2041 accgttgctg gtcagtctaa tgccatggcg tatggcgaag gactgccatt accggacagg
     2101 gaagatgcgc ctcatcccag aatcaaacaa ttagcgagat ttacgcatac gcatcccgga
     2161 ggcccgccat gtcactttaa cgacattatt ccactgactc actgcccaca cgatgttcag
     2221 gatatgcagg gttatcacca tcctctggca acgaatcatc aaacacagta cggcaccgtt
     2281 ggccaggcac tgcatattgc acggaaatta ctgcccttca ttcctgataa tgcagggatt
     2341 ctcatcgtcc cgtgttgccg tggcggatcg gcttttaccg cgggcagcga ggggacatat
     2401 tcagaacggc acggagccag ccatgatgct tgtcgttggg gaacggatac cccgctctac
     2461 caggatttag tcagcagaac gcgagccgca ctggcaaaaa atccgcataa caaattcctc
     2521 ggcgtatgct ggatgcaagg cgaatttgac ttaatgacca gtgactacgc gtcacaccct
     2581 caacacttta atcatatggt tgaagccttt cgtagggatc taaaacaatt ccattctcag
     2641 cttaataata ttactgccgc gccgtggttt tgcggcgata ccacctggta ctggaaagaa
     2701 aatttccctc atgcgtatga agctatttat ggcaattatc aaaataatgt tttagccaat
     2761 attattttcg tcgacttcca gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa
     2821 gatccggacg atttaagcac gggatatttc ggttcagcgt accggtcagc ggagaactgg
     2881 acgacgtcac tgcgaagcag tcatttcagc gcggcagccc gtcgggggat tatttctgac
     2941 agatttgtag aagcaatttt gcagttttgg cgcgaaaggt gagcggggag tttattcatg
     3001 ggggatgtcc ttgtccattc ccagagcaga caacaaaaaa caaatgagat caaggtaata
     3061 tctcttcact tgcgggctcg aacagcaggc aattgccaat ataataatct ggcacaaggg
     3121 tgaaaaacgc gcaccgcata aaccgttgtt attgcttaca gattagccgg atgccttaat
     3181 agacatccgc gccttttcgg ttatggctcg gaaatctacg aacatctaca cagtttactg
     3241 gaacgttttg ggccacagcg ttcgcagcac cgtcctgata tgccgttctg gcgattataa
     3301 gtcgacggat tctggcaaat agacgatgat gatatgctta atgatgttca aagaaattag
     3361 gccattatta tattat
//
LOCUS       DACADF010000271         3319 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.275, whole genome shotgun sequence.
ACCESSION   DACADF010000271 DACADF010000000
VERSION     DACADF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.275"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            291..1607
                     /gene="shiA"
                     /locus_tag="ISB30_004736"
     CDS             291..1607
                     /gene="shiA"
                     /locus_tag="ISB30_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HAP1992276.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            1709..3163
                     /gene="amn"
                     /locus_tag="ISB30_004737"
     CDS             1709..3163
                     /gene="amn"
                     /locus_tag="ISB30_004737"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAP1992277.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          767 a          801 c          804 g          947 t
ORIGIN      
        1 tataatgcaa tgcagtctat cagaatcatc agggtgcaat tttgtgcata tgcggacttt
       61 tatgtttcat atctcgaacc tgtgggccct ctgcttaatc cttaaacaac accagcaact
      121 cctgcgcttt catcttccat cgaatttttc atgttgccgc taatcagcca taaaatcatt
      181 tgcagatgcg ctctgtcgag gtagtctcat aaggttcgtt tatagatcga cggcaatgtg
      241 agttaccttt tccatactaa ttataaaaag acagtacaaa caggatcatt atggactcca
      301 cgctcatctc cactcgtccc gatgaaggga cgctttcgtt aagtcgcgcc cgacgagctg
      361 cgttaggcag tttcgctggt gccgtcgtcg actggtatga ttttctactc tatggcatta
      421 ccgccgcact ggtgtttaat cgcgagtttt tcccgcaagt aagcccggcg atgggaacgc
      481 tcgccgcatt tgccaccttt ggtgtcggct tccttttccg accgctcggc ggtgtcattt
      541 tcggtcactt tggcgaccga ctgggacgta agcgcatgtt aatgctgacc gtctggatga
      601 tgggcatcgc gacagcctta attggtattc ttccttcatt ctcgaccatt gggtggtggg
      661 cacctatttt gctggtgaca ctgcgtgcca ttcagggatt tgccgtcggc ggcgaatggg
      721 gaggcgcagc gttgctttcc gttgaaagtg cgccgaaaaa taaaaaagcc ttttacagta
      781 gcggtgtaca agttggctac ggtgtaggtt tactgctttc aaccggactg gtttcattga
      841 tcagtatgat gacgactgac gaacagtttt taagctgggg ctggcgcatt cctttcctgt
      901 ttagcatcgt actggtactg ggagcattgt gggtgcgcaa tggcatggag gagtccgcgg
      961 aatttgaaca acagcaacat aatcaagcgg ccgcgaaaaa acgcatcccg gttatcgaag
     1021 ctctgttacg acatcccggt gctttcctga agattattgc actacgactg tgcgagttgc
     1081 tgacgatgta cattgttact gcctttgcac ttaattattc aacccagaat atggggttac
     1141 cgcgcgaact tttccttaat attggtttgc tggtaggtgg attaagctgc ctgacaattc
     1201 cctgttttgc ctggcttgcc gatcgttttg gtcggcgcag ggtttatatt acaggcgcgt
     1261 tgatcggaac gttgagcgca tttcctttct ttatggcgct tgaagcacaa tctattttct
     1321 ggatagtttt cttctccata atgctggcaa acattgcgca tgacatggtg gtgtgtgtgc
     1381 aacaaccgat gtttaccgaa atgtttggtg ccagttatcg ctacagtggt gctggagtcg
     1441 gttatcaggt tgccagtgtg gttggcggtg gatttacacc ttttattgcc gctgcactca
     1501 tcacttactt tgccgggaac tggcatagcg tcgccattta tttgctggct ggatgcctga
     1561 tttctgcaat gaccgcttta ttgatgaaag acaatcaacg ctcgtgatag cctggcgaag
     1621 atcatccgat cttcgcctta tacttttgtt tcacattcct gtgacatact atcggatgtg
     1681 cggtaattgt atggaacagg agacacacat gaataataag ggctccggtc tgaccccagc
     1741 tcaggcactg gataaactcg acgcgctgta tgagcaatct gtagttgctt tacgcaacgc
     1801 cattggcaaa tatattacaa gtggcgaatt acctgatgaa aacgcccgca aacaaggtct
     1861 ttttgtctat ccatcactga ccgtaacctg ggacggtagc acaactaatc cccccaaaac
     1921 gcgcgcattt ggtcgtttta cccacgcagg cagctacacc accacgatta ctcgccctac
     1981 tctctttcgt tcgtatctca atgaacaact tacgttgctg tatcaggatt atggtgcgca
     2041 tatctcggtg caaccctcgc agcatgaaat cccttatcct tatgtcatcg atggctctga
     2101 attgacactt gatcgctcaa tgagcgctgg gttaactcgc tacttcccga caacagaact
     2161 ggcgcaaatt ggcgatgaaa ctgcagacgg catttatcat ccaacggaat tctccccgct
     2221 atcgcatttt gatgctcgcc gggttgattt ttccctcgca cggttgcgcc attataccgg
     2281 tacgccagta gaacattttc aaccgttcgt cttgtttacc aactacacac gttatgtgga
     2341 tgaattcgtt cgttggggat gcagccagat cctcgatcct gatagtccct acattgccct
     2401 ttcttgtgct ggcgggaact ggatcactgc cgaaaccgaa gcgccagaag aagccatttc
     2461 cgaccttgca tggaaaaaac accagatgcc agcatggcat ttaattaccg ctgatggtca
     2521 gggtattact ctggtgaata ttggcgtggg accgtccaat gctaaaacca tctgcgatca
     2581 tctggcagtg ctacgcccgg atgtctggtt gatgattggt cactgtggcg gattacgtga
     2641 aagccaggcc attggagatt atgtccttgc acacgcttat ttacgcgatg accacgttct
     2701 tgatgcggtt ctgccgcccg atattcctat tccgagcatt gctgaagtgc aacgtgcgct
     2761 ttatgacgct accaagttag tcagcggcag gcctggtgaa gaagtcaaac agcggttacg
     2821 tactggcacg gtggtaacca cagatgacag gaactgggag ttacgttact cagcttctgc
     2881 acttcgtttt aacttaagcc gggccgtagc aattgatatg gaaagtgcaa ccattgccgc
     2941 gcaaggatat cgtttccgcg tgccatacgg gacactactg tgtgtttcag ataaaccgtt
     3001 acatggtgag attaagcttc ccggccaggc taaccgtttt tatgaaggcg ctatttccga
     3061 acacctgcaa attggcattc gggcgatcga tttgctgcgc gcagaaggcg accgactgca
     3121 ttcacgtaaa ttacgaacct ttaatgagcc gccgttccga taataagtta tgaaccttct
     3181 tatcagaaat agatgtgaag gattaagtaa gacatctggc aaatagcctg caataaacgc
     3241 gggctgtttg cttccttggg cagatgcgag ttttattatc gtcttaatga tttccacata
     3301 ttaaaagcaa gtatgcttt
//
LOCUS       DACADF010000272         3317 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.276, whole genome shotgun sequence.
ACCESSION   DACADF010000272 DACADF010000000
VERSION     DACADF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.276"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            212..1732
                     /locus_tag="ISB30_004738"
     CDS             212..1732
                     /locus_tag="ISB30_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAP1992278.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1757..2095)
                     /gene="maoP"
                     /locus_tag="ISB30_004739"
     CDS             complement(1757..2095)
                     /gene="maoP"
                     /locus_tag="ISB30_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAP1992279.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2214..3053
                     /gene="hdfR"
                     /locus_tag="ISB30_004740"
     CDS             2214..3053
                     /gene="hdfR"
                     /locus_tag="ISB30_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAP1992280.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(3149..3224)
                     /locus_tag="ISB30_004741"
     tRNA            complement(3149..3224)
                     /locus_tag="ISB30_004741"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3189..3191),aa:Trp,seq:cca)
     gene            complement(3233..3309)
                     /locus_tag="ISB30_004742"
     tRNA            complement(3233..3309)
                     /locus_tag="ISB30_004742"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3273..3275),aa:Asp,seq:gtc)
BASE COUNT          891 a          815 c          812 g          799 t
ORIGIN      
        1 agtgagtgaa aaaaattcaa tttcagtatg aatgttgttc ccttcctcgt agttcatcct
       61 ttgcccgctg catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca
      121 caaataaaca ggaatcagcc actaaaattc acgaatttgc gggctacagc agcgatatct
      181 tatgcacata atggtgactc aaggagggct tatgtcactg tcaattgttc atacccgcgc
      241 agccctggga gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc
      301 aggcttaacg atggtgggct taccagaaac aacggtaaaa gaagctcgcg atcgcgtgcg
      361 cagcgccatt atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc
      421 tccagccgat ctgccaaaag aagggggacg atatgatttg cctatcgcta ttgcgttgct
      481 ggcggcctca gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact
      541 ggcgcttaca ggcgctctgc gtggcgttcc cggcgcaatc tccagtgcaa ctgaagctat
      601 taagtcgggc agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa
      661 cggtgaagga tgcctgatag ccgatcatct gcaggctgtc tgcgcgtttc tggaaggtaa
      721 gcacgctctc gaacacccga aaccaactaa tgcagtatcc cgggcgctac aacatgatct
      781 cagtgatgtt gtcggtcagg agcaaggaaa gcgaggactg gaaattaccg ccgctggcgg
      841 gcacaacctt ttactgattg gaccgccggg aacaggtaaa acaatgctcg ccagccgtat
      901 taatggcctt ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag
      961 tctggtaaat gctgaatcag tacaaaaaca atggcggcaa cgcccgttcc gctcacctca
     1021 tcacagtgcg tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat
     1081 ttcgctggcg cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac
     1141 actggatgcc ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc
     1201 aaaaataacc tatccagccc gtttccagct tgtcgcggcg atgaatccca gccctaccgg
     1261 acattatcag ggaaaccata accgctgcac accagaacag acattacgtt atctcaaccg
     1321 gctctcgggg ccctttctcg accgcttcga tctctcactg gagatcccat taccgcctcc
     1381 cggcattttg agtaaaacgg tagtgccggg agaaaacagc accaccgtta aacaacgtgt
     1441 aatggccgcc agagagcgcc aatttaagcg gcagaataaa ctgaacgcct ggctggataa
     1501 tccggaaata cgccaattct gcaagcttga gagcgaagat gcgcagtggc tggaagaaac
     1561 gctgatccat ctggggttat cgattcgtgc ctggcagcgg ttattgaaag ttgcacgaac
     1621 cattgctgat attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag
     1681 ctatcgtgcg attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg
     1741 catttcgccc tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg
     1801 gtttaccgcc ggaaagggtg tgaaaacgtt ttggacgctt gatacgcgtc atatacttgg
     1861 accacacgcg ttctgcttct gtcactggtt cacgttcgcc acggcatact gctacgaaga
     1921 gtttctcttc ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac
     1981 catgacgctc aagcagttgt gcctctttga tggtgaagtc accatgacga gagaatccac
     2041 gtggataatg tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc
     2101 acgctcctaa ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga
     2161 gtgtaaaaca aaacatttaa atcataacga caaataattt tgcggagagc actgtggata
     2221 cggaattgtt aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg
     2281 aatcgctcta tctgacccaa tcagccgtga gctttcgaat cagacaactg gaaaatcaac
     2341 tgggtgtgaa tcttttcacc cgccacagaa acaatatccg tttaaccgct gccggagaaa
     2401 agcttctgcc ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg
     2461 cgcatacctc acgacataat gagttttcta tcggtgccag cgcctcgctg tgggagtgta
     2521 tgcttaatca gtggctggga cgcttgtatc aaaatcagga tgtccataca ggcttacagt
     2581 tcgaagcgcg aattgcccaa cggcagtctc tggtaaaaca gttgcatgaa cgccagcttg
     2641 atcttcttat tacaactgaa gcccccaaaa tggacgaatt ttgtagtcag ttgctggggt
     2701 atttcacttt agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc
     2761 tgcgactaga gtgggggcca gactttcaac agcatgaggc aggtttgatc ggtgctgacg
     2821 aagttcccat tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta
     2881 atggttgcac ctggctaccc gtcagttggg cacgtaaaaa aggcggcctg cataccgttg
     2941 tcgatagcac aacactttca cggccgcttt atgccatatg gctacaaaat agcgataaaa
     3001 atgcgttgat tcgcgatcta ttgaaaatta acgtgctgga tgaagtgtat taatctgaat
     3061 ggctggcaag gatgccggta gaaggattta cttcggagag ggttatttca gataaaaaaa
     3121 atccttagct ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca
     3181 cccggttttg gagaccggtg ctctaccaat tgaactacgc ccctaattag ggtggcggaa
     3241 cggacgggac tcgaacccgc gaccccctgc gtgacaggca ggtattctaa ccgactgaac
     3301 taccgctcca ccgaatt
//
LOCUS       DACADF010000273         3221 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.278, whole genome shotgun sequence.
ACCESSION   DACADF010000273 DACADF010000000
VERSION     DACADF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.278"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(62..589)
                     /gene="mobB"
                     /locus_tag="ISB30_004743"
     CDS             complement(62..589)
                     /gene="mobB"
                     /locus_tag="ISB30_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAP1992281.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(571..1155)
                     /gene="mobA"
                     /locus_tag="ISB30_004744"
     CDS             complement(571..1155)
                     /gene="mobA"
                     /locus_tag="ISB30_004744"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAP1992282.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLTHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1225..1494
                     /locus_tag="ISB30_004745"
     CDS             1225..1494
                     /locus_tag="ISB30_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAP1992283.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1571..2557
                     /gene="srkA"
                     /locus_tag="ISB30_004746"
     CDS             1571..2557
                     /gene="srkA"
                     /locus_tag="ISB30_004746"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAP1992284.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2574..3200
                     /gene="dsbA"
                     /locus_tag="ISB30_004747"
     CDS             2574..3200
                     /gene="dsbA"
                     /locus_tag="ISB30_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAP1992285.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          819 a          779 c          786 g          837 t
ORIGIN      
        1 gcatatgtgc ggaataaatg ccggatgcga cgctggcgcg tttgcttcag cctattcttg
       61 cttatccgtc ctgcttttgc atccactcca ccacaaaatc agccagcccc tcaacatcat
      121 taatatccag taacgcgaca tcaagattaa gcggcacatc actggctaca gcaataacat
      181 gcctgtctat cactaattct tccggtcgat gtccggctcc atcgcgaaac agcacaatct
      241 ttgcgatctc ttcatgctta aacccttcga ccagaatcaa atccagcttt gaggtatcca
      301 ttcgacttgc gagaaaatgt agatccagct cttcttcgtc tggtgtttcc gtcatcaagg
      361 cccatcgctg ctggctggca acgatggttt gcgccgcgcc agccttgcgc agctcatagc
      421 tatctttgcc cggtttatca acatccatat catggtgcgt atgcttaatc agccccggac
      481 ggatccctct ggcgcataat gccgggatca attttttcag cagcgttgtt tttccggtgc
      541 cactccatgc agcaaaggcg agtaacggta tcatcgtttt tcctgccatc gggcaagctc
      601 ctctggcgta ttcacgttta taaatgcctc tttacgatcg ctgaaatcaa ccgcatgacc
      661 gccagccaga cgcataaatg ccataacccg gcgttctcct gcttgcagat attccagtaa
      721 taaaggctca attgcgcggt ttaccagagc aatagtcggg tgatcgcgtt caccgtcgtg
      781 gacccacacg acaggcgcat ctttgcgctg atgagtaagc cgggcggcta aatcatgggg
      841 aatgtaaggc gtatcgcacg ggcaaaacaa aaaccactca cccgcttcct gctgcattac
      901 tgaaagcatt cctgccagag ggccagggta atccgccagt gaatcttcaa tcactttcag
      961 accgcttacc tgatagattt cctgatgacg attagcatta accacgacgt gagaaagctg
     1021 cgtcataagc gcgtcagcga catgttgcca taatggtttg ccgtttaatt caagcaatcc
     1081 tttatctacg ccgcccattc gtctggcttt accgcccgcc agcacaacgc ctgttatcgt
     1141 cgtcatcaga ttcaccgata tcgcctcttt tattgtggga ttgaccctgc taacgtgtct
     1201 gtctcaagag taaggagcat tactatgaaa tgtaaacgtc tgaatgaagt tattgaactc
     1261 ctccagccag cctggcaaaa agagccagac cttaacctgc tgcaattttt gcagaaactg
     1321 gcgaaagagt caggttttga cggcgaactg gcggatttga cggatgacat tctgatctat
     1381 cacctgaaaa tgcgtgattc cgcgaaagat gcggtgatcc cgggtttgca gaaagattat
     1441 gaagaagatt tcaaaacggc gctattacgc gcacgtggcg taattaaaga gtaaaagctt
     1501 gtaagcggcg caaccaaaat catcgtgaaa tgatatcctt catcattcgt aatgttttcc
     1561 ggatgatggg atgaacaaca gcgcttttac tttccagaca ctacacccgg ataccatcat
     1621 ggacgctctg tttgagcatg ggatccgggt ggattccggt cttaccccgc ttaacagcta
     1681 tgaaaaccgt gtctatcaat ttcaggacga agatcgtcga cgttttgtcg tcaaatttta
     1741 tcgccctgaa cgttggacag ccgatcaaat cctcgaagaa catcaatttg cgttgcagct
     1801 ggttaacgat gaagttccgg tcgcagcacc tgtggccttt aatggtcaga ctttattgaa
     1861 ccatcaggga ttttatttcg ctgtttttcc aagcgtcggt ggtcgccagt tcgaagctga
     1921 taatatcgat cagatggaag cggttgggcg ttatttaggg cgtatgcacc agacggggcg
     1981 caaacagctt tttatccatc gcccgaccat cggtttgaat gaatatctca ttgagccacg
     2041 caagctgttt gaggacgcta cactgatacc ttccgggttg aaagcggcat tcctgaaagc
     2101 gacagatgag ctgattgccg ccgttacagc acactggcgg gaagatttca ccgttctgcg
     2161 gctacatgga gactgccacg ccgggaatat tctctggcgc gatggcccaa tgtttgttga
     2221 tctggatgat gcacgtaatg gtccagccgt tcaggatttg tggatgttgc tcaatggcga
     2281 taaagccgag cagcggatgc aactggaaac tattattgag gcttatgaag aatttagcga
     2341 gttcgacacc gctgaaatcg gactgattga acctttacgc gccatgcgtt tggtttatta
     2401 tcttgcctgg ctaatgcggc gttgggctga tcccgcgttc ccgaaaaatt tcccgtggtt
     2461 aaccggggaa gattactggc tacgacagac ggcgactttt atagaacagg caaaagttct
     2521 acaagaaccc cctttgcaat taacacctat gtattaatcg gagagagtag atcatgaaaa
     2581 agatttggct ggcgctggct ggtttagttt tagcgtttag cgcatcggcg gcgcagtatg
     2641 aagatggtaa acagtacact accctggaaa aaccagttgc tggcgcgccg caagtgctgg
     2701 agtttttctc tttcttctgc ccgcactgct atcagtttga agaagttctg catatttctg
     2761 ataacgtgaa gaaaaaactg ccggaaggcg tgaagatgac taaataccac gtcaacttca
     2821 tggggggtga cctgggcaaa gagctgactc aggcatgggc tgtggcgatg gcgctgggcg
     2881 tggaagacaa agtcacagtt ccgctgtttg aaggcgtaca aaaaacccag accattcgtt
     2941 cagcatctga tatccgcgat gtatttatca acgcaggtat taaaggtgaa gagtacgacg
     3001 cggcgtggaa cagcttcgtg gtgaaatctc tggtcgctca gcaggaaaaa gccgcagctg
     3061 acgtgcaatt gcgtggtgtt ccggcgatgt ttgttaacgg taaatatcag ctgaatccgc
     3121 agggtatgga taccagtaat atggatgttt ttgttcagca gtatgctgat actgtgaaat
     3181 atctgtccga gaaaaaataa ttcaatgtaa ataacataaa g
//
LOCUS       DACADF010000274         3199 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.279, whole genome shotgun sequence.
ACCESSION   DACADF010000274 DACADF010000000
VERSION     DACADF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.279"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            62..553
                     /locus_tag="ISB30_004748"
     CDS             62..553
                     /locus_tag="ISB30_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1992286.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     VSSKNEEGSQTASLQMRHEKEGHLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            556..1062
                     /locus_tag="ISB30_004749"
     CDS             556..1062
                     /locus_tag="ISB30_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin HipA"
                     /protein_id="HAP1992287.1"
                     /translation="MALIGVYADWEGLDGPARIGYLHSRRTRAREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERVSRAI
                     EEDPDNKE"
     gene            1062..1823
                     /locus_tag="ISB30_004750"
     CDS             1062..1823
                     /locus_tag="ISB30_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HAP1992288.1"
                     /translation="MGQKWLRMLIAPGGSLGGARPKASVVDEAGHLYIAKFPSVKDEY
                     DVGGWEMVVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLT
                     RHQDGEDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVTNTDDHLRNHGFI
                     LIPGKGWRLSEAYDLNPVVRSDGLKLNITENDNALDLELAREVAEYFRLSLTEADDII
                     ANFRGIVSQWRIIAERLRLPGREQELMAEAFRGAI"
     gene            complement(1966..3183)
                     /locus_tag="ISB30_004751"
     CDS             complement(1966..3183)
                     /locus_tag="ISB30_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1992289.1"
                     /translation="MNYLDRTALGIAAPTIMETTGITKEQYSWIVSAFQLAYTLGQPI
                     MGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLAFMRALMGFSEASAIPAGVKT
                     ASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMFHSWQFAFIVSGSLALLAALF
                     WFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKTSIKHILSQRNFWGIGIARFL
                     ADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLGDLGCLASGFVAKFFHDRGVS
                     LINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIGAFSHQCLSTVAATLGGDLFK
                     KDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPFFIALAFFDIIGAIALWTLIK
                     VKDEEPQVQLATS"
BASE COUNT          921 a          655 c          774 g          849 t
ORIGIN      
        1 caatccaata aatgctaata ttatggctat catagccata aaaagataaa atattatcac
       61 tatgggtaaa aaaacagcac cgttactccc ttatacgaat cagatgctta gtgaatttgg
      121 tgagcggctg aaacttgctc gtttacgacg caggcttacc gcaaagcaag ttgctgaacg
      181 tgctgggatg tcgccaataa cattgcgctc tctggagtca ggaagtgcag gggtcacgat
      241 aggggcatat ttgtctgtga tgcaggtttt gggcctggaa acggatttaa ataagcttgc
      301 agctgaagac gacttagggc gtcagctcca ggattcccgt ttgattaaaa aaagagggct
      361 gtctcgtgta tcaagtaaga atgaagaagg ttctcagaca gcctcattgc agatgaggca
      421 cgagaaagaa ggtcatctca ccacggattt aacagcactg gcagaaggct cgacgccatc
      481 tattacagga gaaacatcaa cctctcttgc tgcactatta aaaccgataa aaaaaccgtt
      541 aaaaggaaat taattatggc attaatcgga gtgtatgcgg attgggaagg cctggatggt
      601 cctgcgcgta ttggttattt acacagtcgt cgcactcggg cgcgtgagat atttgagttt
      661 gagtacgata aaaaggcgct tgcagatcct tcacttaatt tcattcagtt agatcctgaa
      721 ataatgttgt atgagggagc acagtaccca attcctccaa aggataaatt tggtgcattt
      781 agtgattcct gcccggatcg ttgggggcgt atgctaatga agcgtcgttt tgagagggat
      841 attcgagacg ggttatgtga taaagactcc cacctttatg agtctgatta tctactaggt
      901 gttcatgatc tttatcgggt tggggctctt cgatacaaac gtgaagacgc tggagagttt
      961 cttgataatc gcattgatgt tgcagcccca ccatttaccg aaatagcgag tctggaaagg
     1021 gttagccggg caattgaaga agatcctgat aataaagagt aatggggcaa aaatggttaa
     1081 gaatgctaat cgctccgggg ggatcattag ggggggcacg tccgaaagcc agtgttgttg
     1141 atgaagctgg gcatctctac atcgctaaat ttcccagtgt aaaagatgag tatgatgttg
     1201 gtggatggga aatggtcgtt aatgcacttg ctgttggctg tggactgaat gttgcacctg
     1261 ctcaggcgca taaatttgcc agcaattacc attgttttat ggttcgtcgt tttgaccgca
     1321 ctaatgcggg aaggcgtctg cattttgctt cggctatgac actaaccagg catcaggatg
     1381 gtgaagatgc ctctacaggt gtgagttatc tggaattagc agatgttctt atcagacatg
     1441 gcgcgcagac taatactgac cttaaggaac tctggtccag gattgttttt aatattcttg
     1501 ttactaatac cgatgatcat ttacgcaatc atggatttat cttgatacca ggaaaaggtt
     1561 ggcggctttc tgaggcatat gatttgaatc ctgtggtcag gtctgatggt ctaaaactca
     1621 atattacgga aaatgacaat gcacttgatt tggaattagc tagagaagtt gcagagtatt
     1681 ttcgacttag tttgacggaa gctgatgaca ttattgctaa ttttagaggg attgtcagtc
     1741 agtggcgaat cattgccgag cgtcttcgat tacctggtcg tgagcaagag ttaatggcag
     1801 aggcgttcag gggggcaatt tagaatagtt atttcgatat attcgctaaa tcataaattt
     1861 gcgaagcaat gccagtagat actgaacgtt ttggaagaga cgtttaattt atgagaaaaa
     1921 tagaaacgct acaaagctaa attatagcgt ttctatgtaa aataattagc ttgtcgctaa
     1981 ctgtacttgt ggttcttcgt ctttaacttt gataagcgtc cacagcgcaa tggcgccaat
     2041 aatgtcaaag aaagccaggg caataaagaa cggcgcgaag ccgataatgt gaacgaatgc
     2101 cccgataaac aggttgaaaa tcaactgacc gctccatgca caggctcctg ccatacccac
     2161 agcggtagct acttcgtctt ttttgaatag gtcaccgccc agagttgctg caacagtaga
     2221 aagacattga tgcgagaatg cgccaatgct aatcagtaat acggcgatgt agggattttc
     2281 gacaatactc accagaccaa tcgtcatcat aatgacggct gcaatagtga aggtaattct
     2341 tcgtgagtta attaaactca cgccgcgatc gtggaagaac ttcgcgacaa aaccactggc
     2401 taaacagccg agatcgccca gcaggaaagg tagccagacg aacatggcaa tttctttcag
     2461 gctaaaatgc agcgtttcga cgaagaaaat cggtacccag aagttaatgg ttccccatgc
     2521 cgggtcagcg agaaaacgcg cgatgccaat cccccagaaa ttacgttggc tgaggatatg
     2581 cttgattgac gttttttctt tcttatctgt tttaagatgc tgttcttgcc cagactcaat
     2641 atagtggcgc tcttcatcag aaaggcgttt agcatctttt ggatctttat aacaaaagaa
     2701 ccaaaataaa gccgcgagta aagcaaggct acctgagaca ataaacgcaa attgccagct
     2761 atgaaacata atgcaccagg caatcaacgg tggagcaagc atcgcgccga gtgaggtgcc
     2821 catattgaaa acacctgtcg ccacgccacg ctcttttgcc gggaaccatg ttgatgcggt
     2881 ttttacaccc gcaggaattg ccgatgcttc gctgaaaccc atcagggcgc gcataaatgc
     2941 cagacctgac catgttcctg tcaaggcatg ccccattgtc gccaggcccc agattgcggc
     3001 acatatcgca aaacttaact tcagacccac ggtatcaata aagaagccca ttatcggttg
     3061 ccccaatgta taggccaact ggaatgcact gacaatccat gaatattgtt ctttggttat
     3121 tccggttgtc tccataatcg ttggagccgc aatacctaac gccgttctgt ccagataatt
     3181 gattacagtg ataaagcat
//
LOCUS       DACADF010000275         3184 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.280, whole genome shotgun sequence.
ACCESSION   DACADF010000275 DACADF010000000
VERSION     DACADF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.280"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(77..390)
                     /locus_tag="ISB30_004752"
                     /pseudo
     CDS             complement(77..390)
                     /locus_tag="ISB30_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            795..1028
                     /locus_tag="ISB30_004753"
     CDS             795..1028
                     /locus_tag="ISB30_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAP1992290.1"
                     /translation="MSEYMKNEILEFLNRHNGGKTAEIAEALAVTDYQARYYLLLLEK
                     EGVVQRSPLRRGMATYWLLKGEKQAGQNCSSTT"
     gene            1495..1710
                     /locus_tag="ISB30_004754"
     CDS             1495..1710
                     /locus_tag="ISB30_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992291.1"
                     /translation="MCCGHRLIPASVRYDQILLNRPGFPGECFICELRLPDHCFRWKH
                     NKLFLLLPEEYGPAFPAIIDCYTSPPT"
     gene            complement(1679..2805)
                     /locus_tag="ISB30_004755"
     CDS             complement(join(1679..2481,2481..2805))
                     /locus_tag="ISB30_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558190.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1992292.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFAKAEF
                     DRLWKKLMPLLDKLREQYGVGPVCSEQHIAPSTYYHCQQQRHHPDKRSARCQRDDWLK
                     REIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVS
                     RKTVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWWVSSS
                     METTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYMERLKEAKLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNR"
     gene            complement(2896..3009)
                     /locus_tag="ISB30_004756"
     CDS             complement(2896..3009)
                     /locus_tag="ISB30_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992293.1"
                     /translation="MKMKHLRDDVAAGRRRLYIRAICPSLREAPLLYSYPL"
BASE COUNT          876 a          804 c          658 g          846 t
ORIGIN      
        1 gaatactgat ataacctgtc tgctttctga caagggcttc tttttttatc ttccagaaac
       61 tccggaatgc cataatttag tcaaatgccg atgactcatc tgtataataa ggtgcaagcc
      121 ttgctgcaag agcattcagc cgtataagtc tccccagtgt tgtactgaaa tacccattat
      181 tcatctggta tttctcgcag acctccttac gggagtgccc acctaccaga tagtccttca
      241 tagccagaat gaccctgtca ctgtgaatag aagaaatacc tatcagtaaa aaaaatgcat
      301 ttcagacata gagccgagca acagcacgct ctcgcgtata ttcagcaaaa acgcatttcc
      361 tgaccgactg atgacttcat gatgcgccat gtttccccct tctgtcgggc ccctggatat
      421 atgcttccgg cccggaaata ctcttcacgc aataagttaa atttagtttt ttatgttgta
      481 aatattgatt taaacgatct tttaacccac aaaacaaaat taaaccagaa taaaattcta
      541 acaacaagaa tacactcaga taaaaacatc atggtgaatt gaatttacag cataaaagat
      601 cgtctaaatg catttttacg gactttcttt tcacagaaaa ataacacaca aaatgtgata
      661 cacatcaaat aaagccaaca aaaagcaaat tcactaaatt gcacaaataa agcaaacaca
      721 gagaataaaa atacctttcc gttgataaca gagagcatga tttttattat tcgaagaaac
      781 tttgttacag tgagatgagt gaatatatga agaatgaaat actggaattt ctgaacaggc
      841 ataatggagg gaaaaccgca gaaattgcgg aggcgctggc ggtaacggat tatcaggcac
      901 gctattacct gttattactg gaaaaagaag gtgtggttca gcgctctcca ttaaggcgag
      961 gcatggcaac atactggctt ctgaaaggag agaagcaggc ggggcagaac tgttcttcca
     1021 caacttaata actcaatctg tgggcatgct tcactgaaac agataaaagt tacatcagag
     1081 tgcagattca gttttgcagc attcatcaca aaatagaaat ttagtatttt atgtcagaat
     1141 acttcacatg ataataaaaa cctgtctgga tttactgcat ggccagacat tacaaaatac
     1201 aaacgccata aacttcattc agccttcttg ctgtccggta acggaggctg ctttttcgtt
     1261 acacctttcc ccggcagcat cagtagcagt aatttataga ttctacgaac tgagactgtc
     1321 tgtccgggga gaacgtacac agcccgacac aatgtaccag tcaataatat ccggtttctg
     1381 attccctgcc tgacagacca ctctctggca actttgtatt cagttcatct actactggag
     1441 acagatttat aaaatccaac tccacgatct ttgaccgtcc gcatccccta ctctctgtgc
     1501 tgcggtcatc ggttaattcc agcgtccgtc cgttacgatc agatattact gaaccgcccc
     1561 gggtttcctg gagagtgttt tatctgtgaa ctcaggctgc cagatcattg tttccgatgg
     1621 aagcataata agctttttct gcttctgccg gaggagtatg gcccagcctt cccagcaatc
     1681 atcgattgtt ataccagtcc acccacgtga gtgtggccag ttccacttct gcacggtttt
     1741 tccagctctt acggtgtatt acctccgctt tgtaaagacc attgatgctc tccgccatcg
     1801 cgttgtcata cgagtcgcct gtactccctg ttgatgccag cagttttgct tcttttaatc
     1861 gctccatata ggccagtgac acatactgag agcctttatc gctgtgatgg atggtgccgg
     1921 acggacggcg ggcccacaac gcctgctcca gcgcatccag cacgaatgtc gtttccatag
     1981 acgatgagac ccaccacccc acgatgtatc cggcaaacac atcaatgatg aacgccacat
     2041 agacgaagcc ctgccatgtg ctgacgtaag taaaatcagc cacccacagc tggtcaggac
     2101 gttctgccac gaactgacgg tttacgcggt cacctgcagc aacggttttc cggctgacgg
     2161 tagtgcggac ctttttaccc cggagaacac cggcaagtcc cataaccgcc atgaggcgcg
     2221 ccactgtaca tctggccacc ctgattcctt cccgtaacaa ctgacgccag actttacgca
     2281 caccgtacac ctgatgattt tcatcgtata cgcgctgtat ctctctcttc agccagtcat
     2341 cgcgctgaca acgggcactg cgtttatcag gatgatgccg ctgctgctga caatggtaat
     2401 acgttgacgg ggcaatatgc tgttcactgc ataccggtcc gaccccgtac tgctcacgca
     2461 gcttatccag cagtggcatt attttttcca gaggcggtcg aactccgcct tcgcaaaata
     2521 agcggaagcc tggcgaagga tatcgttact gcggcgcagt tcacgatttt cacgttccag
     2581 ctctttcaga cgctgacgtt cagcggtggt gagcccacca tcaccgcccc cggtatcccg
     2641 ctcatgctgg cgaacccaga cacgcagagt ctctggcgta cagccaatct ttggagctat
     2701 ggaacaaatt gccgcccatt gtgagtcata ttcgtcctga ctttccagaa ccatacgaac
     2761 tgcccgttga cggacttcgg gggaaaaacg tgtattttta gtcatcctgt ttacctcttt
     2821 ctcagggaat ttagtctcca ggattcccgg ggcggttcac agtaatcatg ccgggtctgg
     2881 ctggctaaca ccccattata aggggtacga ataaagtaac ggggcttcac gcagggatgg
     2941 acagattgct ctgatataca gccgacgacg tcccgctgca acatcatcac gcagatgctt
     3001 cattttcaga tttgggacct ccttttgatt tctcgtttaa cttttgctgt taccagactt
     3061 taatctattt taacaattca agaatttttt ataaaatgct caaagatgaa agcgttactg
     3121 aaccaaccag cttggctgat gttttctata gataaaaaga tcatcagcaa aaactgatag
     3181 cctg
//
LOCUS       DACADF010000276         3171 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.281, whole genome shotgun sequence.
ACCESSION   DACADF010000276 DACADF010000000
VERSION     DACADF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.281"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..211
                     /locus_tag="ISB30_004757"
     CDS             <1..211
                     /locus_tag="ISB30_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAP1992294.1"
                     /translation="MTDGWPLYESRLKRKLHVISKRYTQRIERHNLNLRQHLARLGRK
                     LLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(371..502)
                     /locus_tag="ISB30_004758"
                     /pseudo
     CDS             complement(371..502)
                     /locus_tag="ISB30_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            784..2142
                     /locus_tag="ISB30_004759"
     CDS             784..2142
                     /locus_tag="ISB30_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HAP1992295.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(2381..>3171)
                     /locus_tag="ISB30_004760"
     CDS             complement(2381..>3171)
                     /locus_tag="ISB30_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP1992296.1"
                     /translation="QSEIYGRQGVELSRSLLSGWVDACCRLLSPLEEALHGYVMTDGK
                     LHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNAGSALAPAVWFAYSPDRKGIHPQTHL
                     ACFSGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHVRIPSALTEEALEQIGQ
                     LYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLFFGSGHGGERGALLYS
                     LIGTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT          791 a          827 c          839 g          714 t
ORIGIN      
        1 gatgacggat ggctggccgc tgtatgaatc acgcctgaag agaaagctgc acgttatcag
       61 caagcgttac actcagcgca ttgagcgaca taatctgaat ctgagacaac atctggcaag
      121 gctgggacgg aagttactgt cgttctcaaa atcggtggag ctgcatgaca aggtcatcgg
      181 gcattatctg aacataaaac actatcagta agttggagtc attacctcct cccgaactca
      241 aagctatcat acagataatt actggttaac cctatatagg gcattttaaa gtgtgatctt
      301 atccagcaat aatggacacg cggctaagtg aaccgccccg ggtttcctgg agagtgtttt
      361 atctgtgaac tcaggctgcc agatcatcgt ttccgatgga agcataataa gctttttctg
      421 cttctgccgg tgggatatgg ccctgccttt ccagcaatcg tcgattgtta taccagtcca
      481 cccacgtgag tgtggtcagc tcatcatact gacacgtgca ttacccctac cgcgataatg
      541 caaccgtaca atatagggcg tttcctaaac gcagccctca tcactccaat ccaacaagac
      601 acagcatttt tgatcacgaa gaaagacaaa taatttccgt aaatgtttcc gttgttacaa
      661 ataacaacag tgttttacat ttctttggcc tcaggcccgc gcatgttgcc attcgatgtc
      721 attttcctga ctcgtttctt ctttatggtg tagtgtgatc ttcactttat ggatgctaac
      781 aatatgaaaa gaaaaattat tcccgtgcta atcggttgta cgctctcttt ctctgccctg
      841 gcggcacaac ctacggctga gcgctatgtc gtcagttttc cagaaggcac gcacgtgaac
      901 tacgccggcg catttgccag tgcattcccg aacggccttc cggttgggat aggttctggc
      961 ctgctgttca ccggtaaaca gggcgatgcc ctgacattcg caaccattac cgatcgcggt
     1021 ccgaacgcgg attcaccgaa agaggggaaa aacgaaacta aaattttcgt tacgccagat
     1081 ttcgcgccgc tgctgatgac gattcgtgta caaaacggca aagcggaggc catcgatccc
     1141 cgtcctctgc atgacgataa aggcgcgatc aacggtctgc cgctggcaag cgacgtgatt
     1201 ggctccacca atgaagtggc cttcagcgac acgttacata gactgaaggg ggataatcgc
     1261 gggctggata cggaagggat cactccggat ggtaaaggag gttactggct gtgcgatgag
     1321 tatggcccgt tcctgattaa catcgacagt aaagggaaaa tcctggcaat ccacggtccg
     1381 caggcggcgg aaggggaaaa agcgattgcg ggcggtttgc caaatatcct caaatggcgt
     1441 caggcgaacc gcggctttga aggcctcacc cgtatgccgg acgggcgtat tattgtcgcc
     1501 gtgcaaagta cgctggatat cgacgctaag agcaagaaaa aggcgctgtt tacgcgtctg
     1561 gtgagcttcg atccggcaag cggaaaaacc gcgatgtacg gttatcctat cgacagcgcg
     1621 gcctacagca aaaacagcga cgctaaaatc ggagacatcg tggcgctcga taaccagcac
     1681 atcctgctga tcgaacaggg aagagataaa aacaacagaa tgcgtaacct gatttacgag
     1741 gtcgatctca ataaggcgag cgacctgtca ggcttcgaca agccgggtga gtatccggag
     1801 tttgatgatg agaaaaccct gtcacagcgc ggcattaccc ttgcgcagaa aacgcaggtt
     1861 gttgatctgc gctccttggg ctggcagcag gagaaggctg aagggttggc gctgatcgac
     1921 agcaaaacgc tggcggtggc gaacgataac gactttggcg tgaaggtggc gatgcaacat
     1981 ccggtcgagg gcaagacatt caaggattat cgggtgaacg cggaaggaaa gctgacgctt
     2041 gacgacaagc aggttgaaac gaccctcaga gtgaagccac tggagaaacc ggagtctgac
     2101 agtgaactgt ggattgtgac gctgccggaa gcgttgaaat aagtttataa ggtacggaca
     2161 gcagtatcgc ttagtttttc aaattcatgt atgtgaacaa tattggggtt ccggcgtaga
     2221 cccccaatga aagcgtcacc acgtgaaaac atgcctgcac ggtttacacg tcatctcatg
     2281 gtggcagccg gttaactttc ctgctacacc agtggaaaaa ggccataagc agtccgaaca
     2341 aaagtaagcg tacagcgagg gccgtattga cggggatgtg ttattcagct ggcagtgcta
     2401 tgcgccacgg aagcagttcg ctgacccggt tgaccggcca gtctgctatg acgccaggca
     2461 catggcgaag gtagctttct ggatccacgt cattcagttt gcacgtcccg atcaggctgt
     2521 acagtagcgc tccccgctca ccaccatggc cagagccgaa gaacagggtc ttcatctttt
     2581 cacgcaacca gctttccagg gatttcaaca gcggtttcgt ttttcgctga cgttcagcaa
     2641 gccgctgctc tgccggcatt ccccttatat ccgcctctat ggcgtacaac tgaccgatct
     2701 gctctagggc ttcttccgtc agtgctgacg ggatgcggac gtgcacatcg tggatctttc
     2761 ggcgggcatg agcccagcag gcagcttccg ttatcccacc attgcgatac agctcgttga
     2821 acccggcgta cgcatccgct tgcagcacac cgctgaagca agcaagatga gtctgcggat
     2881 ggatgccttt tctgtccggg ctgtaagcga accacactgc aggtgccaac gctgacccgg
     2941 cattgcggtc atcacgaaca tacgcccaca accgcccggt cttcgtcttc ttattacccg
     3001 gcagcagtac ctggaccggg gtatcatcgg catggagttt gccgtcagtc atgacatagc
     3061 catgaagcgc ctcttccagc ggagacagca gccggcagca tgcatccacc cagcccgaca
     3121 gcagtgaacg gctcagctcc acaccttgcc ggccgtatat ttctgactgg c
//
LOCUS       DACADF010000277         3143 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.282, whole genome shotgun sequence.
ACCESSION   DACADF010000277 DACADF010000000
VERSION     DACADF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.282"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            45..281
                     /locus_tag="ISB30_004761"
     CDS             45..281
                     /locus_tag="ISB30_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAP1992297.1"
                     /translation="MAGQSLSVGFRPFPQTTPTRATAHERTPQQERQKAAREAERGRE
                     AWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(313..939)
                     /gene="tetR(A)"
                     /locus_tag="ISB30_004762"
     CDS             complement(313..939)
                     /gene="tetR(A)"
                     /locus_tag="ISB30_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAP1992298.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGGTVEQAPLS
                     PLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1045..2244
                     /gene="tet(A)"
                     /locus_tag="ISB30_004763"
     CDS             1045..2244
                     /gene="tet(A)"
                     /locus_tag="ISB30_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAP1992299.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2276..3136)
                     /locus_tag="ISB30_004764"
     CDS             complement(2276..3136)
                     /locus_tag="ISB30_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAP1992300.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
BASE COUNT          598 a          987 c          959 g          599 t
ORIGIN      
        1 gacttctcct atcccctggg aacacatcaa tctcaccgga gaatatcgct ggccaaagcc
       61 ttagcgtagg attccgcccc ttcccgcaaa cgaccccgac ccgcgccacg gcccacgagc
      121 ggacgccgca gcaggagcgc cagaaggccg ccagagaggc cgagcgcggc cgtgaggctt
      181 ggacgctagg gcagggcatg aaaaagcccg tagcgggctg ctacgggcgt ctgacgcggt
      241 ggaaaggggg aggggatgtt gtctacatgg ctctgctgta gtgagtgggt tgcgctccgg
      301 cagcggtcct gatcaatcgt caccctttct cggtccttca acgttcctga caacgagcct
      361 ccttttcgcc aatccatcga caatcaccgc gagtccctgc tcgaacgctg cgtccggacc
      421 ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc gagagcggag cctgttcaac
      481 ggtgccgccg gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag
      541 cgcattgacg gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc
      601 ggccgtttcc atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg
      661 gtaggcgagc agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc
      721 gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc
      781 gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa
      841 ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag
      901 ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat
      961 aaacataata tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag
     1021 cggaggctgg tccggaggcc agacgtgaaa cccaacagac ccctgatcgt aattctgagc
     1081 actgtcgcgc tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg
     1141 cgcgatctgg ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat
     1201 gcgttgatgc aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg
     1261 cggccggtct tgctcgtctc gctggccggc gctgctgtcg actacgccat catggcgacg
     1321 gcgcctttcc tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact
     1381 ggggcggtag ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac
     1441 ttcggcttca tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg
     1501 ctgatgggcg gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc
     1561 aatttcctga cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta
     1621 cgccgggagg ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc
     1681 gccgccctga tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt
     1741 tgggtcattt tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt
     1801 gccgcatttg gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc
     1861 cggctcggcg aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc
     1921 ctgcttgcct tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg
     1981 ggtggcatcg gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt
     2041 caggggcagc tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc
     2101 ctcctcttca cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt
     2161 gcaggcgctg ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc
     2221 gcagggcaac gagccgatcg ctgatcgtgg aaacgatagg cctatgccat gcgggtcaag
     2281 gcgacttccg gcaagctata cgcgccctag gagtgcggtt ggaacgttgg cccagccaga
     2341 tactcccgat cacgagcagg acgccgatga tttgaagcgc actcagcgtc tgatccaaga
     2401 acaaccatcc tagcaacacg gcggtccccg ggctgagaaa gcccagtaag gaaacaactg
     2461 taggttcgag tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc gctccgatca
     2521 ggccgagcca cgccaggccg agaacattgg ttcctgtagg catcgggatt ggcggatcaa
     2581 acactaaagc tactggaacg agcagaagtc ctccggccgc cagttgccag gcggtaaagg
     2641 tgagcagagg cacgggaggt tgccacttgc gggtcagcac ggttccgaac gccatggaaa
     2701 ccgcccccgc caggcccgct gcgacgccga caggatctag cgctgcgttt ggtgtcaaca
     2761 ccaacagcgc cacgcccgca gttccgcaaa tagcccccag gaccgccatc aatcgtatcg
     2821 ggctacctag cagagcggca gagatgaaca cgaccatcag cggctgcaca gcgcctaccg
     2881 tcgccgcgac cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg
     2941 aaatattaag tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc
     3001 ggacgatcat cacgagcaat aaacccgccg gcaacgcccg cagcatcgcg accgtcatcg
     3061 gtgagaagtt cggcaggtat tgggtggtga caatgtaggt gctgccccaa atggcaggtg
     3121 ctatcgctgt gaacaataaa tcg
//
LOCUS       DACADF010000278         3135 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.283, whole genome shotgun sequence.
ACCESSION   DACADF010000278 DACADF010000000
VERSION     DACADF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.283"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(239..1252)
                     /locus_tag="ISB30_004765"
     CDS             complement(239..1252)
                     /locus_tag="ISB30_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 domain-containing protein"
                     /protein_id="HAP1992301.1"
                     /translation="MSTRIYLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHSLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVMGFGHHYLAQPTVVLHRSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDLPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(1264..2580)
                     /gene="fucP"
                     /locus_tag="ISB30_004766"
     CDS             complement(1264..2580)
                     /gene="fucP"
                     /locus_tag="ISB30_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAP1992302.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNQHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRNALAEVTES"
     gene            complement(2608..>3135)
                     /locus_tag="ISB30_004767"
     CDS             complement(2608..>3135)
                     /locus_tag="ISB30_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP1992303.1"
                     /translation="KLIVLQLEVQLETVYHAIEFGKKNGIEVLLNPAPALRELDMSYA
                     CKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNIIVTMSEKGALWMTRDQ
                     EVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAALFAAFSVTGKGTQSS
                     YPSIEQFNEFLTLNE"
BASE COUNT          952 a          719 c          670 g          794 t
ORIGIN      
        1 tttttgttga ccactacaaa taaataaaag agttgcagtg cttgctcggt aagaaaacaa
       61 tggaagttga gatcctaaaa gaagctgtgg agtacaccac tctacagtag ttgcgcaaat
      121 ttggagtgga cgataatgtg atcgtctata agggcaacgc tatcatagtc ttgtcctggc
      181 gggtaaaaaa acgcgcttac cttaacgata agcgcgccgc tgttcaggcc ttaagtggtt
      241 attcaattcc tgtggtgact gtaaaagtgc gtgtttgctg cggtgcaacc tgaatcagcg
      301 tgccattacg ttgcgcggca agatacccct caggccgaca ggttgcaggt aatgcaaagg
      361 cggctacctg ttgctcgccg ttataaagga tccagcgtgt cacataattt agttcggcac
      421 tggagaaacg agtaacaaac gtagtgccat cgggagcgat catgcgaaac tctggcagat
      481 ctgtgtaagc gtccagtttg tctgcaaaga agactatttc tggatcataa aattccggtt
      541 gattcagcgt cgacagagag gcttctccct gcataatccg ttgattaaac gccagccact
      601 gagcggtggg attaacatgc gaaggtactg attcacgcaa ccttaatatt tcgtccggta
      661 tattctggct gaacgtagca tttggtatat atgcataatt catgtggcac atatattgta
      721 gtggcatatc tacagaagcc agattggtta cggccatctt aatatcgaac aatgtagagg
      781 atctgtgaag gaccactgtt ggctgagcca gataatgatg cccgaacccc attacatact
      841 cgtaacgccc ggtaaggcgt aacatatctc cctctaattc catccatgct tcatccatcg
      901 cggcacaggc catttcaccg tgtagcagat gagtatcttc cgcagatggg cagccattag
      961 ccagcaaacc tgaatgaaag gcaaaacagc cataggtttc tatcacctct gtcgccggtt
     1021 taggctggcg aaacatattg cacatggtga gactgtgtcc atcaaattgc gcatcccaaa
     1081 tcatctgccc catccaggga agaataatta aatgtccacg actgtttgca attttaagcc
     1141 cctcgacacc actgtcatag cgaaaagacg tgacagtaaa atcactattt tccagcaaga
     1201 tacgaggttt ttcgccaaaa agcgcccgcc acaaataaat acgcgtactc ataacgattc
     1261 tcctcaggac tctgtgactt cagccagtgc attacgtact ttgctttcac gccagaagta
     1321 gacaccgaca tagacaaaac agagcataga aaccaggaat gaaagctgta gtgagtggaa
     1381 catatctgca atatacccct gaattgccgg aaccaccgct gcgccaacaa tagccataac
     1441 aatgactgct cctgccattt ctgtatgttg attatcaact gtatccagtg ttcctgcata
     1501 gatcgtcgcc cagcaagggc caaacaaaac acttaccagg acggcgacat agaccgcgct
     1561 gaaacttgga gccagtgcaa catatgccag aaacagcgcc cctataacgg aatagagaat
     1621 taggactttt tccggattaa aacgtgtcat aaggatgttg gctataaact tgccaataaa
     1681 gaagcaggca aagctgtaga ccatgaagtt tgaagcatca cgttcgttga tatcgcccaa
     1741 ctccagtgcc agacggatgg taaatgacca tactgcgacc tgcataccca cataaaggaa
     1801 ttgagccaca ataccacgac gaaagcgcga atttttagcc agatagcgca gcgtatccat
     1861 tgctgacggg cgtttatggt gacttgtctg tgccacttta caggttggga agcgggttaa
     1921 aaggaacaac accatgacca caaccagaat cataatcata tacttatacg gttcaagggt
     1981 gttctctaac atcagcacct taaagttgtg aatttgctcg gcgttcattc cggacatctg
     2041 cttctcaagg ctttccccct cggagaaaac cagatatttg cccaataaaa taccagacgc
     2101 agcaccaatc ggataaaagg tctggctgat attgagccgc aatgtggcat aggcttttgg
     2161 accgatcatt gaactgtatg tgttcgctgc agtttcaagg aaactcaggc caatcgcaat
     2221 cgcaaaaata gctgcaagga acatagtgta ggttgccata tgcgaggcag ggaaaaaaag
     2281 tgtacaacca ccaatataca gcgtcaggcc aattaaaatt gccaccttat aactggtctt
     2341 tttaatcaca agggatgctg gtattgcaat taaaaaataa cctccataaa atgcgctctg
     2401 caccaatgct gaagcaaagt tacttagcga aaatacactt ttgaattgag tgattaatat
     2461 gtcatttaat gcagctgcgc atccccatag cgggaataaa cacgataaca aaataaactg
     2521 gaacaaggga gtcttattca gatacccatc aggcatctga atgatgtttt tatcgttcat
     2581 agtgctacct ttaactgtgc aggatgatta ttcgtttaag gttaaaaatt cattaaattg
     2641 ttcaatactc ggataagatg attgcgtacc tttccctgtg acgctgaaag cggcaaagag
     2701 agcggctttt ttcaaagcgg cttcaacatc accgctttga acataataat gggaaaagca
     2761 accaataaat gcgtcaccag cgccactagt atcaacagca tttactttga atgcaggaac
     2821 atggacttcc tgatcgcggg tcatccataa tgcgcctttt tcgctcatgg taacaataat
     2881 attgttcagc cctttatcaa ctaacgaacg tgcggccaaa cgaatatgat cataagtatc
     2941 aaccgacata ccggttaata tttccagttc tgtttcattc gggataaaga aatcacattt
     3001 gcaggcataa gacatatcta actcacgcaa tgccggagcc ggatttaata acacttcaat
     3061 accatttttc ttaccaaact caatcgcgtg gtaaactgtt tccagttgaa cttccagttg
     3121 taaaacgatc aattt
//
LOCUS       DACADF010000279         3114 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.284, whole genome shotgun sequence.
ACCESSION   DACADF010000279 DACADF010000000
VERSION     DACADF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.284"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            109..1002
                     /locus_tag="ISB30_004768"
     CDS             109..1002
                     /locus_tag="ISB30_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1992304.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(1033..2022)
                     /locus_tag="ISB30_004769"
     CDS             complement(1033..2022)
                     /locus_tag="ISB30_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP1992305.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAADKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLNRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2049..2900)
                     /locus_tag="ISB30_004770"
     CDS             complement(2049..2900)
                     /locus_tag="ISB30_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP1992306.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
BASE COUNT          755 a          746 c          794 g          819 t
ORIGIN      
        1 cgatacagcc agattatcat tgttactaat aatgtggtta aaataatagc atctatgccc
       61 cccttcatga gtgtctgtga agaggatgct tctacaagga aatcgccaat gctcaaagaa
      121 aacttcaacg aactgcaaat ctttcttgtg gtggcaagag agcgaagttt taccaaagca
      181 gcgggcaaac tcggcgtctc tcagtctgca cttagccatg caatgaaggc tctggaggaa
      241 aggctcaata tccgcctcct gacacggact acccgcagtg tggctcccac agaagcgggg
      301 gagagaatca ttgcctgcct tgaaccgcgt attgacgagc ttgaacagga actggaatca
      361 cttattcaat tgaacggcac gccctccggg aatattcggt tatctgccgg ggaacatgcc
      421 gcgcgaagtc tggtctggcc gaagctaaaa ccttttctca gagagtatcc cgaaatcaat
      481 gtcgaactgg tggtggataa cggttttgtc gatattgttg aaggccgttt tgatgccggg
      541 atacgactgg gtgaaaatgt tgataaagat atggttgcag tcaggattgg accagatatg
      601 cggatggctg tggtgggagc tccggcgtat tttgctgcaa accctgcgcc tgaaacgcct
      661 catgagctac aaaatcatcg ctgcatcaac atgcgtttgc caactgccgg tgggctttac
      721 cactgggagt tcgagcgaga agggaaaccg cttcgcgtta aggttgatgg gcaactgacg
      781 tgtagtccat tgccagaaag aattgatgcc gcgttatcag ggtttgggat cgcttgtgtc
      841 cctgaagaca tggttcagga gtatataaag tcaggcaagc ttattcaggt gttacaggag
      901 tggtgtccaa cttttcctgg gtattatctt tactacccca gccgcaagca gcatccgcca
      961 gcttttgcgc tgttgatcga tgcgcttcgc tacaccgaat aacgggtcct ttatcctgga
     1021 cccgtttggg tattagcggc ctacgcgtgc ctgatgttca ggcgagtaac gttcaccgac
     1081 gattttaatt gtttccagcg cctcggttat ctgctgcgta tctttctgcg aaagaacaat
     1141 gtcagcagcg gccaggtttt cttccagccg gttcagttta gtggtgccag ggatgggaac
     1201 aatccacggc ttttgtgcta atagccatgc cagtgcgatt tgtgcagacg ttaccccttt
     1261 atctgcagcc agttcaccta acaaggtgac cagtttttca ttggcttcaa tggcttgtgc
     1321 tgcgaaacgc ggtacggtgc tgcggtaatc atctttgccg aaagtggttc ccggcttaat
     1381 cgcccctgtc aggaaacctt tacctagtgg gctgaagggg acaaaaccaa ttcccagttc
     1441 ctccagcaac ggcagaattt cctgttcagg ctcacgccac cacatggaat attcgctttg
     1501 cagggcggtg acaggttgta ccgcatgtgc acgacgaatg gtttgtgctc ctgcttcaga
     1561 caggccgaaa tgtttgacct taccttctgc gatgaggtct ttcaccgttc ctgcaacatc
     1621 ttcaatagga acatctggat caacacggtg ttgatacagc aggtcaataa catctgtttt
     1681 cagacgacgt aatgatcctt caacggcttc acggatatgt tccggacggc tgtttaaaat
     1741 ctgctgctta ttatcatcgc cgaaggtaaa accgaattta gtggcaatta ccacgcggtc
     1801 acgaaacggt tttaaggctt cgccgaccac ttcttcattc agatacggac catacacttc
     1861 ggcggtatcg aagaaggtga caccacgttc aaccgcagca cgaataagtt cgatagcctg
     1921 acgggtatcg gtcgccgggc cgtagccgtg gcttaagccc atgcaaccga gcccaagggc
     1981 ggagacttcg agtccggatt tacccagata acgtttttgc attgaatgaa tacctctttt
     2041 atcgcggctt aaacatcaag tttgcggcca gtcagccatt ccaccattgc gggatcgcgg
     2101 tgagagaaga acgcgctggt tgcggtatcc agggcggtaa tttgcagcat atcttcaggg
     2161 ctgagttcaa aatcgagaat gttgatgttc tcttccatgc gttcttttcg cactgatttt
     2221 gccagtgaaa caatgcctcg ttggtagatc cagcgcagaa caacctggcc cacgcttttg
     2281 ccatacttct cgccaattgc cgtgagaaca ggatgctgga acagaccatt tttcccttcc
     2341 gcaaacggtg cccaggcttc tggctgaatg ccacgacttt gcatccaggg aacggcctgt
     2401 agctgctgat tgaaagggtt aacttcaacc tggtttaccg ccggaacgac gttattaaaa
     2461 gcgataaggt ctgccagtcg gtcaggatgg aagttactga cgccaatagc gcgaatttta
     2521 ccggcctgtt gcagctcttc catggcacgc caggctccgt ggacatcgcc atatggctgg
     2581 tgaatcaagt acaggtcaac gtaatcaagt tgcagtcgat tcagagagcg ttcgaactgg
     2641 gctttggccc cttcgtaact ggtatcctgc aaccagagtt tcgtggtgac aaagagttcg
     2701 ttacgggcga taccgctttg tttcagtgcg ttcccgacct ggatttcatt ttgataagag
     2761 gcggcggtgt caatcagtcg ataaccgctg ttaatagcgt caatgaccgc tctttcgcat
     2821 tcggcggcat cggtcatctg aaatacacca aagcccaaca ggggcatttc aataccgttg
     2881 ttcagtttta ctttttgcat ggtgttatcc ttccgttatc gttcatgaga agcataacgt
     2941 aaagggaaga gctcgattag acggcaggat ttgtcagggg ttatgaacaa aattcataaa
     3001 tctgtttgag ggttgagatt agtcgtgcaa gttcgatatc cccggaattt acagtccgat
     3061 gaataaaaat gaatgtccat aaaaaatata ttggtttatc ccggcgtata tact
//
LOCUS       DACADF010000280         3058 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.285, whole genome shotgun sequence.
ACCESSION   DACADF010000280 DACADF010000000
VERSION     DACADF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.285"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            26..253
                     /locus_tag="ISB30_004771"
     CDS             26..253
                     /locus_tag="ISB30_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992307.1"
                     /translation="MQDPSLRTYRIAFLGSNASGNLPMFTRVQATTGKRAIKAFIERC
                     EPVKGWFLGEPEDITDQLKKEEEEAGSKPQI"
     gene            345..575
                     /locus_tag="ISB30_004772"
     CDS             345..575
                     /locus_tag="ISB30_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992308.1"
                     /translation="MIVSIFSPSAGAVKPRRHSRILRADITAPEIDPALRAFGRHIAR
                     SHRKGRGVHIPAMKNTALGQVLRTLELKRAFN"
     gene            627..1982
                     /locus_tag="ISB30_004773"
     CDS             627..1982
                     /locus_tag="ISB30_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HAP1992309.1"
                     /translation="MTPTTAQIMTENTVSQTYRATYSPDDNKLRLYASLRLDEETYSL
                     INKAGFRWAPKQELFVAPAWTPGREDVLLSLAGDIEDEDSTLFDRQEQRAGRFSDYSD
                     RRAVESEQALAHVDSLASAVPLGQPILVGHHSERRARRHAQKIENGMKRAVMLFERAE
                     YWEQRAQASLRHAKYKERPDVRYRRIKKIEAELRKSQKHIARSEKYMTMWRAQTLDLK
                     MALLVSNYDHIHACFTLDKYPRPTEKSQYEGSMSLHSALSEEIITFEQARDIAIRCHE
                     RTISHQQRWVNHYQNRLAYERAMLNENGGVVTRTEEFEPGGQVLSRGEWLTILRVNRS
                     QGEVSSVETPCYRFLGYSGTMKLTPDRITDYKAPTAEEASDAKKAAKRPPIVNYPGEG
                     FREMTKAEWAKLPADYKGVRGAAETETHGAYRFRRCMTHGCTLVNVYITDMKTVEIPK
                     K"
     gene            2030..2593
                     /locus_tag="ISB30_004774"
     CDS             2030..2593
                     /locus_tag="ISB30_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HAP1992310.1"
                     /translation="MHHDLKHRIQAMRDKLEGRAPVAEIQGSSQLFVTPAPECRRLVE
                     LADVRETDRILEPSAGTGAILQAIRDTVPRAKCDAVELHAGLVSHLQARFPEVRTWCG
                     DFLEYHPDRRYTRIIMNPPFHRGDDIRHIRRALTLLEPGGILTGICLDGPRQQKALES
                     LADVWEPLPRGTFTYTQVATAILRITV"
     gene            2937..>3058
                     /locus_tag="ISB30_004775"
     CDS             2937..>3058
                     /locus_tag="ISB30_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992311.1"
                     /translation="MSDVKVNGSACCDRPGRAAFTVPAIKLPALCGATPFRARAL"
BASE COUNT          703 a          882 c          879 g          594 t
ORIGIN      
        1 tctttaaaaa tcaggaaaaa gaaaaatgca ggatccttca ttgcgaacgt atcggatcgc
       61 atttctggga agcaatgcca gcggcaatct gccgatgttt acccgggtgc aggccacgac
      121 cggaaaacgg gcgataaaag cgtttattga acgctgtgaa cccgtcaaag gctggtttct
      181 gggcgaaccg gaagacatta ccgatcagct taagaaggag gaagaagaag cgggaagcaa
      241 accgcagata tgaaaaaagc agggtaaacc ctgcttctcc cacgccgtaa aaatcgcacg
      301 aacggttttt ccggcttcgt taactaccat aaggagtcaa ctctatgatc gtttctattt
      361 tttcaccttc cgcaggcgct gtaaagcccc gtcgtcattc gcgcattctg cgcgcagata
      421 tcaccgcacc tgagattgat cccgctctca gagctttcgg gcgtcatatc gccagaagtc
      481 accgcaaagg gcgcggtgtc cacatccccg cgatgaaaaa taccgccctc gggcaggtac
      541 tccgcacgct ggaactgaaa cgcgcattta actgatgagg tgagccgccc cgcgaggggc
      601 ggcaactgca ggaaggagaa aacacaatga caccgactac cgcacagatc atgacggaaa
      661 ataccgtcag ccagacttac cgggcaactt attcacccga tgataataaa ttgcgacttt
      721 acgcatcatt acggctcgat gaagaaacgt attccctgat caacaaagca ggctttcgct
      781 gggcgcccaa acaggaactc tttgtggcgc cagcctggac gccggggcgg gaggatgtcc
      841 tgctttccct cgccggtgat atcgaggacg aggacagcac cttgtttgat cgccaggagc
      901 agcgcgccgg gcggttcagt gactacagtg accggcgggc ggttgaatcg gagcaggcgc
      961 ttgcgcatgt tgattcgctg gcatcggctg tcccgctggg acagccgatc ctggtaggcc
     1021 accacagcga gcgccgcgcg cgccggcatg cgcagaaaat tgagaacggc atgaaacgtg
     1081 cggtgatgct gttcgagcgc gccgaatact gggaacagcg cgcgcaggcc tctctgcggc
     1141 acgcgaaata caaggagcgt ccggatgtgc gttaccgccg tatcaaaaaa atcgaggcgg
     1201 aattacgcaa gtcccaaaag cacattgccc ggtctgaaaa gtatatgacg atgtggcgtg
     1261 cgcagacgct cgatctgaaa atggcgttac tggtcagtaa ttatgatcat attcatgcct
     1321 gcttcacgct cgacaaatac ccccgcccga cagagaaaag ccagtatgag ggctcgatgt
     1381 ctctccattc tgccctgtct gaggagatca ttacttttga acaggcgcgg gatattgcga
     1441 tccgctgtca tgagcgcacg attagccacc aacagcgctg ggttaatcat taccagaacc
     1501 gcctggccta cgaacgcgcg atgcttaatg aaaacggtgg cgttgtcacc cgaacagagg
     1561 aatttgaacc aggcgggcag gtactgagcc ggggagaatg gttaaccatt ctccgggtta
     1621 accggagtca gggggaggtg agttcagtcg aaacgccctg ttatcgcttt ctgggctaca
     1681 gcggcacgat gaaactcacg cccgaccgta tcacggacta caaagccccg acagcggagg
     1741 aagccagcga cgcgaagaaa gcggcgaagc gcccaccgat cgtcaactat ccgggggaag
     1801 ggttccggga aatgacaaag gccgagtggg cgaaattacc cgctgattat aaaggcgtgc
     1861 ggggtgctgc agaaaccgaa acacacggcg cttaccgctt ccgccggtgc atgacgcacg
     1921 gctgtacgct cgtgaatgtg tatatcacag acatgaaaac cgttgaaatt ccgaaaaaat
     1981 gacaggaagg ctccccggca tcttgccggg gagatcaggg agtacattca tgcatcatga
     2041 ccttaaacac cgtattcagg cgatgcggga caaactggag gggcgcgccc ctgtcgctga
     2101 aattcagggg agctcgcagc tttttgtcac ccccgccccg gaatgtcgcc ggctggttga
     2161 gctggctgat gtccgggaaa ccgatcgcat tctggaaccc agcgcaggaa cgggcgccat
     2221 actgcaggcg atcagggata cagtcccccg ggcgaagtgt gacgccgtgg agctgcacgc
     2281 cggattagtc agccacctgc aggcacgttt cccggaggtc cggacctggt gcggggattt
     2341 tctcgaatat catccggatc gccggtatac ccgaatcatc atgaatccgc cgtttcatcg
     2401 cggtgatgat atccggcata tccggcgggc attaactctg cttgagcctg gcgggattct
     2461 gacgggcata tgtctggacg gcccgcgtca gcaaaaggcg ctggagtccc tggcggacgt
     2521 ctgggagccg ttgccgcggg gaacattcac gtatacccag gtagcgaccg ccatcctcag
     2581 aataaccgtc tgatgacacg ttaagccggc ggggcacctg tccccgtctg gccgcgcctc
     2641 gaaagaccgc gcgcctgcgg cgccgggaaa accgggccgc gctgacgcgc cgcccgcatt
     2701 tcctttccgc gtcttccctg tttgctgcct gctgtctgtc tgcttcctct tcttctccct
     2761 gctgtgatgc ctctttttaa cccttaaagg ctttaaaagc acacccacac tggacacctt
     2821 cggctcctgg ccagctacgc tgtccggtcg tcgagccaca ctccgctaac cgcgaaaaga
     2881 cgcggtaagc tccgccgaac ccgctacgcg gtttcgggcc tggcggctag tgtctgatgt
     2941 ctgacgtcaa ggtcaacggc agcgcttgct gcgaccgtcc cggtcgcgcc gctttcaccg
     3001 tgccggcgat aaagctgccg gcactctgcg gggcaacgcc gtttcgcgcc cgcgctct
//
LOCUS       DACADF010000281         3008 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.286, whole genome shotgun sequence.
ACCESSION   DACADF010000281 DACADF010000000
VERSION     DACADF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.286"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..413)
                     /locus_tag="ISB30_004776"
     CDS             complement(<1..413)
                     /locus_tag="ISB30_004776"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="catalyzes the formation of pyruvate and
                     N-acetylmannosamine from N-acetylneuraminic acid; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAP1992312.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVY"
     gene            565..702
                     /locus_tag="ISB30_004777"
     CDS             565..702
                     /locus_tag="ISB30_004777"
                     /inference="COORDINATES: protein motif:HMM:NF024407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992313.1"
                     /translation="MQCPVTILCVTLCDYLAPELITHSPEESDTVTLRKRRLPLEMMA
                     W"
     gene            1074..1499
                     /locus_tag="ISB30_004778"
     CDS             1074..1499
                     /locus_tag="ISB30_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP1992314.1"
                     /translation="MEQKILSAEPRRTFSNEFKLQMVKLASQLGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSDAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            1496..1846
                     /gene="tnpB"
                     /locus_tag="ISB30_004779"
     CDS             1496..1846
                     /gene="tnpB"
                     /locus_tag="ISB30_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP1992315.1"
                     /translation="MISLPSDTRISLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRSDMIKILWADADGLCLFTKRLEEGLFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            1877..2974
                     /locus_tag="ISB30_004780"
                     /pseudo
     CDS             1877..2974
                     /locus_tag="ISB30_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          789 a          774 c          739 g          706 t
ORIGIN      
        1 ttataaacta ccatcggaag tccatctgca gaatcaataa tcttcctgta atggatacaa
       61 tgctcttcaa atgaaaaagg ataataaaat ggagtaacag cagatatagc atgatatccg
      121 tatttttttg cagcttttgc aagtacctcg ctttctgccg tactgattcc gcccacatgc
      181 gctataagtg tcagtcttcc atctgattca tcggctactg tttcaagaat tttttctcgc
      241 tctgcaacat tctgaagaaa tgcttctcca gtagagcctc caacatacaa tcccttaata
      301 ttttgttcga tattaaaacg gatcagtttt cgaagactct ccatatcgat ttgctgggat
      361 tggtcataag gagtgggtaa tgctgatatg acccctttaa attcacattg catttttaat
      421 caccttcact taactgcata atgggatacc tagtacacca tcaagtacaa agattatcca
      481 acagaaaaga agcacaatgt gaagattatc aaaaaaaaac catcagaaag ctgtcatgcc
      541 gctcagttaa ggatcggttg atcgatccag tgtccagtta ctattctctg cgtaaccctc
      601 tgcgactatc ttgccccgga actgattacc cacagccctg aagagtccga cactgtcacg
      661 ctacgcaaac ggcgtcttcc tctggaaatg atggcctggt gaattatcag tatggcccat
      721 gagcgtaagg aaccttcacc agattgtgaa ccggctggac acatgctgcc aggtagtcgc
      781 ccctttgtcg cgccgtgatc catgcccagc agggccagga agcggggccg tacgccacgt
      841 gttcacgtaa acagcgcagc cctgacataa caccacgctg tatccgctct ggtgcaatat
      901 gaacctgctg accatcgatg gcgtgttcta actaacacag gacacgccag aacacgacat
      961 aaagcttcac tcgccagata catggtaagc gtaaactgac cgccgtatgt agccattaag
     1021 ccagtattgg taacgtaggt gcccaccttt tcatcaacta gtggacacct gttatggaac
     1081 agaaaatatt atccgcagaa ccccgcagaa cattttcaaa tgagtttaaa cttcaaatgg
     1141 ttaaactggc ttcacaacta ggagcctctg ttgcccgtat tgccagggaa cacgatatca
     1201 atgataacct cctgttcaaa tggctcaggc tctggcagaa cgaagggcgt atatcgcggc
     1261 gtcttccggt aacaacctct tctgacgctg gcgttgaatt attacctgtt gagataacgc
     1321 ctgatgagca gaaagagcct atggcggctc ttactccgct tttatccact ccttcacaaa
     1381 gcacagtcag tgccagttcc tgtaaagttg agttccgcca cggtaacatg acgctggaaa
     1441 atccttcacc agagctgctc acggtgttga tccgtgaact gaccgggagg gggcgatgat
     1501 ctcgctccca tcagacactc gaatctcgct ggttgccggc gttactgata tgcgtaaatc
     1561 cttcaacggt ctgggtgaac aggtacaaca tgtgctggat gagaacccct tctccggtca
     1621 cctgttcatc ttccgtggcc gacggagtga catgattaaa atcctctggg ctgatgctga
     1681 tggtctgtgc ctgttcacca aacgcctgga ggaaggcctg tttatctggc ctgcggtgcg
     1741 tgacggtaag gtatccatta cccgctcgca gctggcaatg ctcctcgata agctggactg
     1801 gcgtcagcca aaaacatccc gtcttaacgc actgacaatg ttgtaaaaat gtcatggccg
     1861 gattataaaa acggtcatga gtcggaaata cctcattcgt atcactgagc tggaaaggct
     1921 gctctctgag caggctgaag ccctccgtca gagagatctg caactgagtc tggttgaaga
     1981 gacggaggcc ttcctgcgct ctgcactggc acgtgccgaa gaaaagatcg aagaggagga
     2041 gcgggaaata gaatatctga gggcccagat agaaaaactg cgccggatgc tgttcggtac
     2101 ccgttctgaa aaactgcagc gtgaggttga acaggctgag gcccaactga aacaacgcga
     2161 acaggaaagc gatcgttaca gtgggcgtga ggatgacccg caggttcccc gccagttgcg
     2221 acagtcacgt catcgtcgtc cgttaccagc acatcttccc cgtgaaatac accgcctgga
     2281 gcctgaagaa agttgttgcc cggagtgtgg cagtgagctg gattatctgg gtgaagtcag
     2341 tgctgagcag ctggaactgg tgagtagcgc cctgaaagtg atccgcactg ttcgggtaaa
     2401 aaaagcctgt acaaaatgtg actgtatagt tgaagcgcca gcgccgtccc gcccgatagc
     2461 gcgcggcatc gcgggctccg gattacttgc ccgcgtgtta acgggaaaat actgcgaaca
     2521 cctgccactg tatcgtcaga gtgaaatctt tgcccgacag ggtgccgaac tgagccgtgc
     2581 attaatctcc aactgggttg acgcgtgctg ccagttaatg actccgctaa atgatgccct
     2641 gtaccgttac gtgatgaaca cccgcaaggt tcacactgac gacacaccgg taaaagtgct
     2701 gacaccgggc agaaaaaagg caaaaacagg acgcatctgg acgtatgtcc gggatgaccg
     2761 gaatgcgggc tcatcagagc caccggcggt ctggttcgcc tactcaccag acaggcaggg
     2821 aaaacatccg gtacaacacc ttcgtccctt ccggggtatc ctgcaggcgg atgcattcag
     2881 cggttacgat cggctgttca gtgccaaacg tgaaggtgat gcacagacag aagttgcgtg
     2941 ctgggtccat gcccggcgaa aaatacacga tgtatgaacg acatctcttc tgacgacatt
     3001 ttcctgct
//
LOCUS       DACADF010000282         3002 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.287, whole genome shotgun sequence.
ACCESSION   DACADF010000282 DACADF010000000
VERSION     DACADF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.287"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..2278
                     /locus_tag="ISB30_004781"
     CDS             <1..2278
                     /locus_tag="ISB30_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP1992316.1"
                     /translation="WGQEYLDKRNITFILSSDKKRLIPRFTKADLREFGLKVDDIPAL
                     QVMDDDTEFGDIAQIIDGARYDFQLDSQTLCLRIPQIYQNARAAGSISPKYWSDGESA
                     VWLSYYASGSRQNSDGDNLNSNWLNLNSGINLGVWRLRNNTVYSDSSWESISTSLQRD
                     IKALRSQMEVGQTFTNGDLFDSVQMTGIKLETDTSMLPDSEQGFAPVVRGIANSDAQV
                     VIKQNGYVIYQTWVSAGPFEIKDLSQVTAGADLEVTIKETNGQEHSFIQASSTVPILQ
                     REGALKYSLATGKYRDNDNHAETPVFGVATAIYGLPYGITIYGGILGASIYHSGVTGI
                     GADLGRLGSVSVDITAAETKFDDGRDDATGLSWRAQYAKDFPDTDTTVTLASYRYSTS
                     QFYTFQEALDQRDTPDDKGIYSYRQTNNRRNRLQINLSQNIGRWGSVYLNGYQQDYWG
                     MHGAERSIGMGYSTTWSNINWSVNYTLTKTPGMAGEQQFSLTLNIPLSRWLPDSWAMY
                     NVNRSDKSNTSHQLGIGGTALQDNNLSYNLQQSYTDNNVGYDASMNGRYRSSVGEFGL
                     GYSYDKNSRQWNYSAQGAVVAHAHGVTLGQSVQDSFAIVHINEGANVKVQNAQGVYTD
                     YWGNAIVPNMTNYRHNAITVNTQGHDSLDISDATQDVIPSKGAVVGVDFDARSGIRAL
                     LTLVHNKERVPFGALLTLGNSTAIVGEDGEVYITGVQESMTFTVQWGKEINQQCTGVV
                     TVPEKYTTGIYKATMDCR"
     gene            2300..>3002
                     /locus_tag="ISB30_004782"
     CDS             2300..>3002
                     /locus_tag="ISB30_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP1992317.1"
                     /translation="MKPGFVSYIRTLLTIIFLSWGVIPEVFAGCNISGNGGNPATVQF
                     SVPALVVNSDAVPGTIIATVNANSPSISVSCTANGEIWQGYTVLTNGDMRTDNPLNNV
                     FQTNVPGIGFRAAWANNTSAQLTAGYLMTPWHKGSSTVIKGYGYPITIHAAIQFIVTG
                     PVGNGTIDTSKLVADWKYDNRVIGELRFSSVPVNIQPGTCDLIEKNITVPLNDIGTDD
                     FDVNNVSPVVSDDRFK"
BASE COUNT          872 a          572 c          743 g          815 t
ORIGIN      
        1 gtgggggcaa gagtatcttg ataagagaaa tattaccttc attctgtctt cggataaaaa
       61 aaggctcatc cctcgtttta caaaggcgga tctccgtgag ttcggtctta aagttgacga
      121 cattcccgcg ttacaagtaa tggatgatga cactgagttt ggtgatattg ctcagattat
      181 tgatggcgca cgttatgatt tccagttaga cagccaaacg ctgtgtctgc ggatcccgca
      241 aatctatcag aatgcgcggg ccgcagggag tatttcaccg aaatactgga gtgatggtga
      301 gagtgcggtt tggttaagtt attacgccag tggttcccgg caaaacagtg atggcgataa
      361 cctgaattca aattggttga atttaaattc agggattaat ttaggcgtat ggcggctacg
      421 aaataatacc gtttacagtg actccagttg ggagtcgatc agcaccagtt tgcaacgcga
      481 tatcaaagca ttaagaagcc agatggaagt cgggcagacg ttcacaaatg gcgatctttt
      541 tgacagtgtg cagatgacag gtataaagct tgaaacggac accagtatgc tgcctgacag
      601 cgaacaaggc tttgcgccag tggttcgtgg tattgccaac agcgatgcac aagttgtgat
      661 taaacaaaac ggctatgtta tttaccaaac gtgggtatcg gcagggccat ttgaaattaa
      721 agacttaagc caggtaacgg cgggggccga tctggaagtc accatcaaag aaaccaacgg
      781 gcaagaacac agttttattc aggccagttc aacggttcca attctacagc gagaaggcgc
      841 attaaaatat agtcttgcaa ctggaaaata ccgtgacaac gataatcatg ctgagacacc
      901 tgtttttggt gttgcaaccg cgatatatgg cttgccctat ggaataacga tttatggcgg
      961 catacttggt gcctcaattt atcattctgg tgtgacaggg attggcgctg atttaggccg
     1021 tctggggtcg gtatctgttg atatcaccgc agcagaaacg aagtttgatg atgggcgtga
     1081 tgatgctaca ggactgtcat ggcgtgcaca gtatgcgaaa gattttccag acactgatac
     1141 gacagtaact ctggcaagtt atcgttattc tacatcacaa ttttacactt tccaggaagc
     1201 acttgatcaa cgtgatacac ctgatgacaa gggtatttac agttatcgac agacaaataa
     1261 tcggcgtaat cgtttacaaa ttaatctttc acaaaatatt ggtcgttggg gctcggtcta
     1321 ccttaacggt tatcaacagg attattgggg aatgcatggg gccgaacgca gtataggaat
     1381 gggatacagt acgacctgga gtaacattaa ttggtctgtt aattacacac taacgaaaac
     1441 gccaggaatg gctggtgagc agcaattttc attaacactc aatattcctc tctctcgctg
     1501 gctgccagat tcatgggcga tgtataacgt aaaccgttcc gataaaagta atacatcaca
     1561 tcaattggga attggcggta ctgctctgca agataacaac ctgagttata atttacagca
     1621 aagttatact gataataatg tgggttatga tgccagcatg aatggacgtt atcgtagctc
     1681 agtgggggaa ttcggtctgg gatatagcta tgataaaaat agtcgtcagt ggaattacag
     1741 tgcgcaaggt gcggtggttg ctcatgctca tggagtaaca ttggggcaat cagtgcagga
     1801 ttcattcgcg atcgtgcata ttaatgaggg tgccaatgta aaagtacaaa atgcgcaagg
     1861 cgtttatact gattattggg gaaacgcgat tgttcccaat atgacgaatt atcgacataa
     1921 cgctattact gtgaatacac aggggcacga tagtcttgat atttctgatg ccacacaaga
     1981 tgttattcct tcgaaagggg ctgttgttgg tgtcgatttt gatgcacgta gcggaatacg
     2041 agccttgttg acattagtcc ataataaaga acgcgtaccc tttggggcat tgctgacatt
     2101 agggaatagt acagcgattg ttggtgaaga tggggaggtt tatattactg gtgtgcaaga
     2161 atcaatgaca tttacggtac agtggggaaa agagattaac cagcaatgta caggagttgt
     2221 cacagtgcca gaaaaataca ccacgggtat ctataaagcc acaatggatt gccgataaca
     2281 aataagacag gaaaaaataa tgaagccagg attcgtctct tatatacgaa cattattgac
     2341 cataatattt cttagctggg gcgtcattcc tgaggttttc gccggttgta acatttctgg
     2401 aaatggagga aatccggcaa cagtacaatt ctccgtgcct gcgttggttg ttaatagcga
     2461 tgccgttcct ggaacgatta ttgcgacagt aaatgcgaac agtccgtcaa tttcagtttc
     2521 ctgtacggca aatggtgaga tctggcaagg atataccgta ttgacaaacg gtgatatgcg
     2581 tactgataac ccacttaaca atgtgtttca gactaatgtg ccaggaatcg gttttcgcgc
     2641 ggcgtgggcg aataacacca gtgcgcaact caccgccgga tatttaatga cgccctggca
     2701 taaaggctcg tcaacagtga ttaaaggata tggttatccg attacaatac atgcagctat
     2761 tcagtttatt gtcacaggac ctgtcggtaa tgggaccatt gataccagca aactggttgc
     2821 tgactggaag tatgacaatc gtgttattgg agagcttcgt tttagttctg ttccggtcaa
     2881 tattcaaccg ggcacatgcg accttataga aaaaaatatt accgttccac tcaatgatat
     2941 tggcacagat gattttgatg ttaacaatgt gtcacctgta gtttccgatg atcggtttaa
     3001 aa
//
LOCUS       DACADF010000283         2998 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.288, whole genome shotgun sequence.
ACCESSION   DACADF010000283 DACADF010000000
VERSION     DACADF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.288"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..125)
                     /locus_tag="ISB30_004783"
     CDS             complement(<1..125)
                     /locus_tag="ISB30_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP1992318.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVD"
     gene            complement(137..367)
                     /gene="yjdI"
                     /locus_tag="ISB30_004784"
     CDS             complement(137..367)
                     /gene="yjdI"
                     /locus_tag="ISB30_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAP1992319.1"
                     /translation="MDQALLDGGYRCYTGENIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVTTVVKVIDTCPSGALKYRHK"
     gene            548..2179
                     /gene="dcuS"
                     /locus_tag="ISB30_004785"
     CDS             548..2179
                     /gene="dcuS"
                     /locus_tag="ISB30_004785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAP1992320.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKAQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2176..2895
                     /gene="dcuR"
                     /locus_tag="ISB30_004786"
     CDS             2176..2895
                     /gene="dcuR"
                     /locus_tag="ISB30_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAP1992321.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
BASE COUNT          800 a          743 c          713 g          742 t
ORIGIN      
        1 tcatcgacat cggtatgttc gataatcgct aaattctctc cggtcggcac aaagacaatt
       61 tcagcgattt gcttgccctg ttcgtcatta atgtaaaatt tattgtggcc ttcgcgtatt
      121 tccattttac cctcgcttat ttatgacggt atttcagcgc gccgcttggg cacgtatcaa
      181 tcactttcac cacagtggtg acgtcgactt catccggcat gatccacggc tttcgtttga
      241 gattaaataa cttgccgttg ccacgtacgc aattgccaga atgctgacat atcgcagtgt
      301 taaaatagac atcgatattt tcgccggtat aacagcgata acccccgtcc agtagcgcct
      361 gatccatgac cttccctctc tcatcagtat ggttttcact ataagcatag ccccggaatc
      421 ttcatacagc actggcagtt tccgttgcca gagtctcttc ccctatatag ccaatcattc
      481 ttactccctt tgaattaccc gcctcatcag agataatgct taagaaattt gtcacacaag
      541 gaagctgatg agacattcat tgccctaccg tatgttacgc aaacgtccga tgaaattgag
      601 taccacagtg atcttaatgg tcagcgcggt actgttctcg gtgctattgg tggtgcatct
      661 gatttacttc tcgcaaatca gtgatatgac gcgagacggg ctagctaaca aggcactggc
      721 agtagcgcgt accctcgccg actcgccgga aatccgtcag ggcttgcaga aaaaagcgca
      781 ggagagtgga atccaggcca tcgcggaagc cgtgcgcaaa cgcaacgatc tgctgtttat
      841 tgtcgttacc gatatgcaaa gtcttcgcta ctcgcatcct gaagcccagc gtattggtca
      901 gccatttaaa ggtgatgaca tccttaaagc gctgaatggc gaagaaaatg tcgctatcaa
      961 tcgcggtttt ctggcgcagg ctttacgcgt atttaccccc atctacgatg aaaatcataa
     1021 acaaattggc gtagtggcga tcggccttga gttaagccgc gtgacccaac agatcaatga
     1081 cagtcgctgg agcattatct ggtcggtact atttggcatg ctggtcggac tgatcggcac
     1141 atgcattctg gttaaagtac tgaaaaaaat ccttttcggc ctggaaccct acgaaatctc
     1201 cacactgttt gagcaacgcc aggccatgtt gcagtccatc aaagaaggcg tcgttgccgt
     1261 ggacgatcgc ggcgaggtca ccctgatcaa cgatgccgca caagaattgc tgaattaccg
     1321 taagtcgcag gacgatgaga aactgtcgac gctaagccac tcgtggtcac aagtggtaga
     1381 tgtctcggaa gtgttacgcg acggaacccc acgccgcgac gaagagatta cgattaaaga
     1441 ccgactatta ctgatcaaca ccgttccggt gcgcagtaat ggcgttatca tcggtgccat
     1501 ttcaaccttc agggacaaaa ctgaagtacg taaactgatg cagcgactcg acggtctggt
     1561 caactatgct gacgcacttc gtgaacgatc ccacgaattt atgaataaat tgcatgtgat
     1621 tctcggatta ttgcatctga agagttataa gcagttggaa gattacattc tcaaaacagc
     1681 caataactat caggaagaga ttggctctct gctgggtaag atcaaatctc cggttatcgc
     1741 tggtttttta atcagcaaga ttaaccgcgc gaccgattta ggccatacgc tgattttaaa
     1801 cagtgaaagc cagctgccag acagcggtag tgaggatcag gtcgcgacgc tgattaccac
     1861 gttgggaaat ctgatagaaa acgcgctgga ggcattagga ccggaacccg gtggcgaaat
     1921 tagcgtaaca ttgcactacc gtcacggctg gctgcactgc gaagttaacg atgatggacc
     1981 ggggatcgca cccgacaaaa tcgatcacat ttttgacaaa ggtgtctcga caaaaggaag
     2041 cgagcgaggc gtcggtttag cacttgtcaa acaacaggta gaaaatctcg gcggcagcat
     2101 cgccgtggaa tcggaacccg ggattttcac acaatttttt gtccagatac cctgggacgg
     2161 ggagaggtcg aacagatgat caatgtatta attatcgatg acgacgcaat ggtcgcggag
     2221 ctgaatcgcc gatacgtggc acaaatcccg ggctttcaat gctgtggaac ggcttcgacg
     2281 ctggagaaag ccaaagagat tatcttcaat agcgatacgc ctatcgacct gatattgcta
     2341 gatatctata tgcaaaaaga gaacgggctc gatttactgc ctgtcctgca taacgcgcgt
     2401 tgcaaaagtg atgtgattgt catctcctcc gctgccgatg cagcaaccat caaagattcg
     2461 ctgcattacg gtgttgtgga ttacctgatc aaacccttcc aggcttcccg ctttgaagag
     2521 gcgctcaccg gctggcggca aaagaaaatg gcgctggaaa aacatcagta ttatgaccag
     2581 gccgagcttg atcagctaat ccacggcagc agctccaacg agcaggatcc tcgtcgcttg
     2641 ccaaaaggct taacgccgca gacgttacgc acgctgtgcc agtggattga cgcgcatcag
     2701 gactatgagt tttcaaccga cgagctggct aacgaggtta acatctcgcg tgtttcctgc
     2761 cgtaaatacc ttatctggct ggtcaactgc cacatcttgt tcaccagtat ccattatggc
     2821 gtcacggggc gtccggttta ccgttaccgt attcaggcag aacactactc actactgaaa
     2881 caatattgcc aataacggat cgctggttat ctgtaagtaa taattattac cgtaatgcaa
     2941 aatacccctg gcacaagtta catcgttcat cagtaaaact tgtgccagat caaatata
//
LOCUS       DACADF010000284         2932 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.289, whole genome shotgun sequence.
ACCESSION   DACADF010000284 DACADF010000000
VERSION     DACADF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.289"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            93..1307
                     /locus_tag="ISB30_004787"
     CDS             93..1307
                     /locus_tag="ISB30_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992322.1"
                     /translation="MPVRLNTIPDAEPYPVPPARIKWLTALLLMLSVGVALTSLFASD
                     ELAKNGPYFWGLACGVPAFIWSLVASVRWLFFITQYIRTDAWNKRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETITAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEVFSRLFSDIRLQLTRLPDDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNHIQEKAVLLVISVRLEPKNPERTAESATALLLANRLTQ
                     TALTPLALLHRPECITDTEMMASGIAQALDWMPVQPDAISGMWTAELDREQRAALLSL
                     NQPFAQEALMYELDAFLGRSGPAAPWLSVAVATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
     gene            1364..>2932
                     /locus_tag="ISB30_004788"
     CDS             1364..>2932
                     /locus_tag="ISB30_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VasK"
                     /protein_id="HAP1992323.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQNFELRRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVALLTALKK
                     LRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGGKRLGFQAPFYLWQVCDNGDYQS
                     GRVLQSVGCLLPEGCTPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAER
                     GITHWQTVLKPLLAGGAFSSLRLRGLMFSPPLAAVPEAAPHAWLPSPVWAGITGDNAR
                     GRAVGFPWLRTALMSAVCVLAIGGAGMTTSFFANRALVQETSIQTARALDTRLPLAEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGPERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADGLRY
                     ENIPEGVRQSVLPSL"
BASE COUNT          592 a          864 c          859 g          617 t
ORIGIN      
        1 gcattaaata aataaaatga ctctatacat aactttaaat ttcacttggt tattaacttc
       61 tggtcgataa tgcaaaataa agggatgtac tgatgccagt ccgacttaat accattcccg
      121 acgcagaacc ctatcccgta ccgccagccc gaattaagtg gctgacagct ttattactga
      181 tgctgtctgt cggtgtggcc ctgacttcat tattcgccag cgatgaactg gcaaaaaacg
      241 gcccgtattt ctgggggctg gcctgtggtg tgcctgcctt tatctggtca ctggttgctt
      301 cagttcgctg gctgtttttt atcacgcagt acattcggac tgatgcctgg aataagcgcc
      361 gggaagaggt gattttgcag gaaacgcgcc ggggacgacg ggcgttgcag atactcagtt
      421 tttccgtaca aaccgcgctg aatggtgatt ccgttgcaga aactataaca gcatttctgg
      481 cacgtcagca ggtgttgaat acctacgctg acttgcgcgg agaagatact gtgcgtcgca
      541 gcgtcatccc tgtattaacc aataaaccgg ttaccggacg tctatccgaa gttttttccc
      601 gtctgttcag cgatatcagg ctgcaactga cgcggctacc cgatgatttt catatcaacg
      661 tactactgga aattgatgcg ccgctttcag gggcctccgt gagaactatc tggcaggacg
      721 aatggcaaaa agccggactc ccggaagccc gtctgttttc agccccggag ccggggctgg
      781 ccgccgtgga cgactggctg gacaaccaca ttcaggaaaa ggccgtactg ctggtgattt
      841 ccgtgcgtct ggaaccgaag aacccggaac ggacggcaga atccgccacc gcgctgttgc
      901 tggcaaaccg gttgacgcaa accgcgctga cgccgctggc cctgctgcat cgtcctgaat
      961 gtatcactga tacagagatg atggcgtcag gcattgcaca ggcgctggac tggatgccgg
     1021 tgcagccaga tgccatatcc ggtatgtgga ctgccgaact ggacagggaa cagcgtgccg
     1081 cgttgttaag cctgaaccag ccgtttgctc aggaagccct gatgtacgag ctggatgcct
     1141 ttctggggcg ctccggaccg gctgcgccgt ggctgtccgt cgctgtggca acgctggcgg
     1201 caatacaatc acaacacccc caactgacgc tcagcggcgt tcagggcggg cattattcat
     1261 gggcgaccgt ggtgtcgcca ttcgtttccc ctcaggaggc atcatagtgg ttccgggatt
     1321 aaaccgtctc cggccccgtt cggtttcagg gcctgccatc gtcatgaccg tggctgttgt
     1381 ggttgcgctt tgcacgttta ttcaccgtaa accggcattg ctgggcgttg aaccgggtag
     1441 ctcacagcaa atgtactgga tggctggcgt gattgccgca ggcgtggtct ttctgattat
     1501 catgggggtt tatcatctgc tgaaccgcca cgctgccgtg cagaactttg aactccgcag
     1561 acagcttgcc gccggagatg actatcgtgc ggtgacgaca aagaaagccg acagccgtca
     1621 tccggcaatg gtggaactcg ccgccttcct gcgcgaccgc tacggcccat tctggcggcg
     1681 taaaatccgc ctgctgctgg tgaccggcga gcctgaacag gcagaggcca tcgcgccggg
     1741 gctgaccggg caacactggc tggagggcga ccacacggtg ctgatatacg gcggcaggcc
     1801 atcagcggag cctgatgtcg cgctactgac cgccttaaaa aaactgcgcc gcagccgtcc
     1861 gctggacggc atcatctggc cgctgacaga agaacagagc cgccagacag cgcaactcga
     1921 caaaggctgg cgcgaactga taaacggcgg taagcgactc ggttttcagg ccccattcta
     1981 tttgtggcag gtctgtgaca acggtgatta tcagtccggg cgcgtcctgc aaagcgtcgg
     2041 ctgcctgctg ccggaaggct gtaccccgga acaactggct gcaatgctgg aagcacagac
     2101 cctaccgctg acggaacagg gcatgtcgca gctgctgacc gataaccgcc atgattttct
     2161 gctgcgtctg gcccataccc ttgcagagcg gggtatcacc cactggcaga ccgtcctgaa
     2221 accgctgctg gcaggcggcg cattttcttc cctgcgcctg cgcggcctga tgttcagccc
     2281 gccgcttgcc gccgtaccgg aggccgcacc tcatgcgtgg ctgccgtcgc cggtctgggc
     2341 aggcatcacc ggcgataacg cgcggggccg cgcggtgggc tttccgtggc tgcgtaccgc
     2401 actgatgtcc gctgtctgcg tgctggcgat agggggggcc ggaatgacga cctcgttctt
     2461 cgccaaccgc gcacttgttc aggaaaccag catccagacg gcgcgggcgc tcgatacccg
     2521 cctgccgctg gcagaacaac tggtggcgct gcataccctg cagggcgaac tggaacgcct
     2581 gcaataccgt atccgcgaag gtgcgccgtg gtatcagcgt tttggccctg aacgtaacca
     2641 gcaactgctc gccgccgcct ttcccggcta tgcgcaggcg gcaaaccggc tggtgcgcga
     2701 cgtggccgtt gaccatctgc aacagcaact gaacgccttt gtcgccctgc cgcccaacag
     2761 tcctcagcgt accgccaccg gtgaacaacg ctataagcag cttaaggcat tgctgatgac
     2821 ttcccgcccg gaaaaggccg acgctgcctt tttcagtacc acgctgatgg cagacggcct
     2881 gcgctatgaa aatatcccgg aaggcgtgcg gcagagcgtg ttgccgtcac tg
//
LOCUS       DACADF010000285         2856 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.290, whole genome shotgun sequence.
ACCESSION   DACADF010000285 DACADF010000000
VERSION     DACADF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.290"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            32..757
                     /locus_tag="ISB30_004789"
     CDS             32..757
                     /locus_tag="ISB30_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP1992324.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            715..1392
                     /gene="bglJ"
                     /locus_tag="ISB30_004790"
     CDS             715..1392
                     /gene="bglJ"
                     /locus_tag="ISB30_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAP1992325.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1430..2218)
                     /gene="fhuF"
                     /locus_tag="ISB30_004791"
     CDS             complement(1430..2218)
                     /gene="fhuF"
                     /locus_tag="ISB30_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAP1992326.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCDDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHAIFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2359..2595
                     /locus_tag="ISB30_004792"
     CDS             2359..2595
                     /locus_tag="ISB30_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAP1992327.1"
                     /translation="MLQRTLGSGWGVLLPGLLITGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2634..2720)
                     /locus_tag="ISB30_004793"
     tRNA            complement(2634..2720)
                     /locus_tag="ISB30_004793"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2684..2686),aa:Leu,seq:cag)
     gene            complement(2755..2841)
                     /locus_tag="ISB30_004794"
     tRNA            complement(2755..2841)
                     /locus_tag="ISB30_004794"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2805..2807),aa:Leu,seq:cag)
BASE COUNT          739 a          640 c          727 g          750 t
ORIGIN      
        1 ataattttta gacagtggat gtggaggaaa tatgttgcca ggatgctgca aaaatggaat
       61 tgttatcagt aaaatacctg ttatgcaagc agggttaaaa gaggtcatga ggactcactt
      121 ccctgaatat gaaataatat ccagcgcctc tgcggaggac cttaccttat tacaattacg
      181 tcgttccgga ttagtcattg ctgatttagc cggtgaaagt gaagatccac gttctgtttg
      241 tgaacattat tattctttaa tctcacaata tcgggaaatt cactgggttt tcatggtgtc
      301 acgctcctgg tattcccagg cagtagaact gctcatgtgc cctacggcga cgttattgtc
      361 tgatgttgaa cccattgaga atctggtcaa gaccgtacgt tccggcaata cgcacgcaga
      421 gcgtatcagc gccatgctga cctctccggc aatgactgaa actcatgatt ttagctatcg
      481 ctccgtcatt ctcactcttt cagagcgcaa ggtactgcgg ctattaggta aaggatgggg
      541 catcaaccag atagcttcat tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa
      601 cagtgcgatg cgtcgactgg caattcacag caacgctgaa atgtatgcat ggataaatag
      661 cgcgcagggt gcaagagaac ttaacttgcc ttctgtttat ggagatgccg cagaatggaa
      721 cacagccgaa ttaagaagag aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc
      781 ggtattgaga gtttattcag aaagtttgcg ggcaaccctt ataagctcca tacctatacc
      841 agtcaggagt catttcagga tgccatgtcg cggatctcgt ttgcggcggt cattttttct
      901 ttttctgcca tgagaagtga gcgcagagag ggattatctt gcctgactga actggcgatt
      961 aagtttccgc gtacccggcg tttagttatt gcggatgatg atattgaagc tcggctgatt
     1021 ggttcattgt cgccatcacc gctggacggt gtattaagta aagcgtcaac gctggagatt
     1081 tttcatcagg aactcttttt gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac
     1141 aatcagtggt acattaacca aagccggacg ctaagcccga cggagagaga aatattgcgc
     1201 tttatgtcgc gtggctactc aatgacacaa attgccgagc agcttaaacg caatatcaaa
     1261 acgatccgcg cgcataaatt taatgtgatg tcgaaactgg gcgtcagttc tgatgcagga
     1321 ttgttggagg ccgcagatat tctgttatgt atgcggcatt gcgaagcaag taatgtgttg
     1381 catccctatt aatccgcatg atgccgggtt tacttccccg gcagtgcttt catttcagcg
     1441 tacaatcgcc acattgctgc acatccggta agcgataacg ctggcagcaa gtgcggcgca
     1501 ccagcaggcc gtcgcgcagt accacggtac gccagagtgg attatcttca ccgttcgtga
     1561 gcgttttctc aaaaaagatg gcatggcgca gcgattcaac cgtcgcctcg ccgagcagtt
     1621 gcttcatctc agtgagatac cagttgatca aataaccggt attactccag ataagtttgc
     1681 cgttgatctc tccggtcgct tctagtgcct gcacaaccgg aaccagcgcc tggctgatta
     1741 acgtttccat tcgctgctgc ggcgaatgtg gtgttgcgtt tttatcgtca cacacatcga
     1801 cccagaaaca ggcggcacgg ccggtttcgt gaaactcagc atggaaatgt tccggcgaca
     1861 catctaatgc tttttcctgc gtcagtagcg ccagcattaa tggtggcacc atcaggccga
     1921 tataccattg tgcccatagt gagatcagcg gtttgttctc gcggatcatc gttggttggt
     1981 tgcgatagat atgatcggaa tagaccgcga gtagagaact tagcgcattc ggtgatgacc
     2041 attgcgccag cgtcatggcg ttaagcggag cgggttcatc caggcggata aactcgagca
     2101 aatgttcacg atgtttggcg atcgtcgcac gtacggcttg tgcaagccca gcatcctgtg
     2161 gctggagatg cgttcgccag ataacatctt catagagcgg tgcggaacga taggccataa
     2221 tcgggatagt aatctaaatg ataatgattg ctaatcctag cgataggttt atccgatagc
     2281 aagggatttc tctggcttgc aaatgataaa aattatcata tgatattggt tatcattatc
     2341 aatgaaagag atgaaaccat gttgcaacgt acgctgggca gtggctgggg agtgttgctg
     2401 ccgggattgc tgattacagg gctgatgtat gcggatttat cgccggatca atggcggatt
     2461 gtcattctga tgggattggt attgacgccg gtgatgctgt atcacaaaca gttacggcat
     2521 tacgttttgc taccatcgtg cctggcgctt attgctggca tcatgctgat gataatgaat
     2581 ttgaatcagg ggtgataaaa cagtgaagag acaagaaaag aagtaaagat aattggtgcg
     2641 agggggggga cttgaacccc cacgtccgta aggacactaa cacctgaagc tagcgcgtct
     2701 accaattccg ccaccttcgc acagtcatct tacttttttt gatattgcct cgtttggtgc
     2761 gagggggggg acttgaaccc ccacgtccgt aagaacacta acacctgaag ctagcgcgtc
     2821 taccaattcc gccaccttcg cccagtgcga gcaata
//
LOCUS       DACADF010000286         2854 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.291, whole genome shotgun sequence.
ACCESSION   DACADF010000286 DACADF010000000
VERSION     DACADF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.291"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(1..135)
                     /locus_tag="ISB30_004795"
                     /pseudo
     CDS             complement(1..135)
                     /locus_tag="ISB30_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164058.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            849..1055
                     /locus_tag="ISB30_004796"
     CDS             849..1055
                     /locus_tag="ISB30_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP1992328.1"
                     /translation="MTTPVSLMDDQMVDMAFITQLNGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            1151..1753
                     /locus_tag="ISB30_004797"
     CDS             1151..1753
                     /locus_tag="ISB30_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992329.1"
                     /translation="MKQQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDNRN"
BASE COUNT          690 a          770 c          652 g          742 t
ORIGIN      
        1 gtcaacggca gagttaaagg cttccagctc cgcaggggta agtttataac gcgtgtggga
       61 aatcactttt tccagcgtct cccgggatga acaacgacgg aactgctgtg gttttaactt
      121 ccattagcct ctcattactt tatgctgcgg ttaacaggat gctgtcagca taccgcacca
      181 gcctggcatt ctggtaccct ccccaaactc attactcccg tgccaccact taaccggtct
      241 ctcagactgt cactcaccag gtcggatacg tttaccgctc agacggatgc ttaatgcact
      301 ctgttcacgt gtgttgcagg tacgttttat atacgcaggt tatacgccag ctatcagaga
      361 tagccctgtg ggtacaacgc aatctgctgc tactgcgttc tcatgcccac tgatcgcact
      421 cacagtgttt atttcacata tatgaactca tgtatttgat ctcatgtgtt tgatctctct
      481 tatatattta taataccaat cagacagtct tatcccacca gtgataactg tcgcctgccc
      541 acaggtcaca ggctgatata ccacaaccgc cttcaaaaac aactctgcaa ttatcagaac
      601 gctagttttc cccttgtctc acgaaagtcc tccctccttt atcgcgtgcg cgtgcggaag
      661 catcgtttcg caacaaccac ccgacatacc ggtacggctc agccaccgca atccccccgt
      721 ttatctcctg acggtcacat tcccgattat tttttacgtt tctccccggt tgttatgccg
      781 gtgaaggtgg tgcgttgttt tcatcaccaa cgccggttga gattaacaac atccggagga
      841 acattctcat gaccacccca gtttcactga tggatgacca gatggtcgac atggcattta
      901 tcactcaact gaacggcctg acagataagt ggttttacag gctcatcagg gatggggcct
      961 ttccggctcc catcaagctg ggccgcagct cccgctggct gaaaagtgaa gtggaagcct
     1021 ggctacaggc gcgtattgca cagccccgcc cgtaatttct gaccgttatc cgttcacccg
     1081 cagcagacgt aatccccggc ctgccggcgg cattctgctg cctgttatat cccccgtgag
     1141 gaatatgaaa atgaaacaac agtatcagac ccgttacgaa ctgctccacg aaagctacca
     1201 gaaatggctg accggcttca cccggcacgc cgtatcctgg ggcgtgtgtc atccgaatat
     1261 ctactacttc cataatctga cgcccgggtg ggtgtcattc aatggtgaaa aaccggagat
     1321 tgccatcgta ccccagagcc tgcaccggct gatttatggc cctgacaaac gggccacgcc
     1381 gcccctggat gatgatctga tagtgaattt atgcaccagt gaacatctgc tggttcatca
     1441 tccgatgctg gaaggcattc tcctgtctga atgtgaacgt ctgaggcagc gttctctggc
     1501 aaataaactg attagcctct tccgtcagtt tggtggcacg gagttgcgcc tcaaactggt
     1561 ctggctttgc tggcttgatt taatgaccgg aaacagcctt gaagactgga aagagaacct
     1621 gaaacggaaa tcagaaaaag agctggagga atggatcatc aaccgtcaga ggcagagcgc
     1681 agcactgacg gatctgatgg atcagtacgt gctcctggca taccgcacaa cggttgacga
     1741 taaccgcaac tgatgccggt tgcttccgga aggtgtgaca aaatacggaa atgatgatgc
     1801 tcccggcccc gcatgctcac ctgcagagcg tgttcgtcgt catcatactg agccatgacg
     1861 ggcgtcttaa gggcatcctg tcattgctga gcaggcaaca ccaccagagg agccacggac
     1921 agggatgttc tttacagccc gatgcacgcc aggttctcag cagactgaat gtgcaggtgc
     1981 ggttattcac cagaaatggc atgctgcggt acatacccat tctggtgaca atatgtacgt
     2041 ggcgacagcg atcccggcac tgtggaagag actcactcag cctgcaggat attctttccg
     2101 gcatccggga atgtccgcct gaatgcaaac aggatgatat ttgaatccgt aatactgttc
     2161 tttcatcata aataatttat gcagatacag ttaaccgtca cagtaccagg tttgttttcc
     2221 cgggaaataa tatccgtcag actgatgccg gaacatatct gaaacacgtt atgatgtttt
     2281 gttatcagaa atgttgtata ttcttatccg tggcgacagt ttaatgccct ctggtatcac
     2341 ctgataatgc atatcctgcg atgattcgtt atatttacgg gacacgcctc ttttcattgt
     2401 gctccttttc agacttcatt ttccgtaagt gttccccggc ataaaggagg tacctttctg
     2461 gcgtttataa ctctctttat cggtgacgcc ggtaatacag ggaataccac accgacaaac
     2521 gaggtattaa cagacgatat ttcagtgtcc ataatttttc tgtccggtta aataacgtca
     2581 gtctgctgcc ctctgactgt aatcatcaaa gccggaatgc ataccgggtt acaacgtaaa
     2641 aaaagtgatg ctggcccccg gtgcagctgg cgggcgaaac atgtttacct ttcaataccc
     2701 cctgtctgca gtctccactc ttccatcctc ttaagctacg tcggccacat gagtgcactt
     2761 ccatgataac aggtattcct gtcagcataa cgaagccggt taccttccct tcctcctgca
     2821 tttcctgtca gaatgactgt acagcgcgca gatt
//
LOCUS       DACADF010000287         2837 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.292, whole genome shotgun sequence.
ACCESSION   DACADF010000287 DACADF010000000
VERSION     DACADF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.292"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            970..1623
                     /locus_tag="ISB30_004798"
     CDS             970..1623
                     /locus_tag="ISB30_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP1992330.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            1716..1973
                     /gene="pemI"
                     /locus_tag="ISB30_004799"
     CDS             1716..1973
                     /gene="pemI"
                     /locus_tag="ISB30_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HAP1992331.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            1906..2307
                     /gene="pemK"
                     /locus_tag="ISB30_004800"
     CDS             1906..2307
                     /gene="pemK"
                     /locus_tag="ISB30_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HAP1992332.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            2592..>2837
                     /locus_tag="ISB30_004801"
     CDS             2592..>2837
                     /locus_tag="ISB30_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAP1992333.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSF"
BASE COUNT          717 a          679 c          692 g          749 t
ORIGIN      
        1 tacagccgcc tggccacagc ttccccctga aagtgacctc ctctgaataa ttcggcacgc
       61 gccggaggca ttcgcacgcc tgaagcccgc cagtgcacaa aaaatcagca ccacatacaa
      121 aaaaacaacc tcatcatcca ccttcaggtg catccggtcc cccttgtttt tgatacaaaa
      181 cacgcctcac agacgggtaa ttttgcttat ccacattaaa ctgcaaggga cttccccata
      241 aggttacaat cgttaatgtc ataaagcgcc agccgtcagc cctacagggt gcaatgtatc
      301 ttttaaacac ctgtttatat ctcctttaaa ctacttaatt acattcattt aaaaagaaaa
      361 cctattcact gcttgtccta tggacagaca gatatgcacc tcccaccgta aacggcgggc
      421 cccaaccgga gccactttag ttacaacaca caaaaccaac ctccagaaaa accccgaacc
      481 agcgcagaac tgaaaccaca aagcccctcc ctcataactg aaaagcggcc ccgccccggc
      541 ccttcgggcc ggaacagagt cgcttttaat tatgaatgtt gtaactacat cttcatcgct
      601 gtcagtcttc tcgctggaag ttctcagtac acgctcgtaa gcggccctca cggcccgcta
      661 acgcggagat acgccccgac ttcgggtaaa ccctcgtcgg gaccactccg accgcgcaca
      721 gaagctctct catggctgaa agcgggtatg gtctggcagg gctggggatg ggtaaggtga
      781 aatctatcaa tcagtaccgg cttacgccgg gcttcggcgg ttttactcct gtatcatatg
      841 aaacaacaga gtgccgcctt ccatgccgct gatgcggcat atcctggtaa cgatatctga
      901 attgttatac atgtgtatat acgtggtaat gacaaaaata ggacaagtta aaaatttaca
      961 ggcgatgcaa tgattcaaac acgtaatcaa tatctgcagt ttatgctggt tatgctggct
     1021 gcatggggca ttagttgggg agccagattt gtcatggagc aggccgttct gctttatgga
     1081 tcaggaaaaa actatttgtt cttcagtcat ggtactgttc tgatgtacct gctgtgtgtt
     1141 ttcctggtat accgccgttg gatagctccg ctaccggtcg ttggtcagct gcgcaacgtt
     1201 ggcgtaccgt ggctggtcgg tgcgatggcc gtggtgtatg tcggtgtatt tctgctcggt
     1261 aaggcgctgg ctctgcctgc tgagccattt atgacgaaac tttttgccga taagtccata
     1321 cctgacgtga tcctgacgtt gctgaccatc tttatccttg ccccgttgaa tgaggaaacg
     1381 ctgttccggg ggattatgct gaacgtcttc cgttcacggt actgctggac gatgtggctg
     1441 ggggcgctga taacgtcgtt gttgttcgtc gccgcgcaca gccagtatca gaacctgctg
     1501 acactggcag aactgttcct ggtggggttg attacatcag tggccaggat cagaagtggt
     1561 ggcctgctgc tgccggtatt gctgcatatg gaagcaacca cgctgggttt actgtttggt
     1621 tgaaagttat atttttatta aacattgtgc gttaaagcct ggtgtgtttt tttagtggat
     1681 gttatattta aatataactt ttatggaggt gaagaatgca taccacccga ctgaagaggg
     1741 ttggcggctc agttatgctg accgtcccac cggcactgct gaatgcgctg tctctgggca
     1801 cagataatga agttggcatg gtcattgata atggccggct gattgttgag ccgtacagac
     1861 gcccgcaata ttcactggct gagctactgg cacagtgtga tccgaatgct gaaatatcag
     1921 ctgaagaacg agaatggctg gatgcaccgg cgactggtca ggaggaaatc tgacatggaa
     1981 agaggggaaa tctggcttgt ctcgcttgat cctaccgcag gtcatgagca gcagggaacg
     2041 cggccggtgc tgattgtcac accggcggcc tttaatcgcg tgacccgcct gcctgttgtt
     2101 gtgcccgtaa ccagcggagg caattttgcc cgcactgccg gctttgcggt gtcgttggat
     2161 ggtgttggca tacgtaccac aggtgttgta cgttgcgatc aaccccggac aattgatatg
     2221 aaagcacggg gcggaaaacg actcgaacgg gttccggaga ctatcatgaa cgaagttctt
     2281 ggccgcctgt ccactattct gacttgaaca tggggtttga ggggcaactg gatgaaaacg
     2341 tacgatttag ggcactaaaa ccgctgttgt cccaccattc tggtgattcc caaacgttat
     2401 ttggctaaaa agtagttttg atgtggttta ttttccgaat tccaagcgca gccctacttt
     2461 cttgtgcgta tttgcgtgtt ttgcgcagtt ttgaagttcc tacatcagat tgacttcgga
     2521 tacaaattca atgaacattg atgcaaatac tgacctatca gtttcttaac taaatactcg
     2581 agatacatct aatggctaat gaaaacttag atattgactc caagacagca gaagaattat
     2641 acgagtggta tctggatgat aaattaatta taaatcgaag ataccaaaga aaattagtat
     2701 ggtccctcga agagaaaaca gcactgattt catcaatgac tcagcaatac cctatccctt
     2761 tgctattgtt tgtttccatt gacaacaaaa gagaaatcct tgatggaatg caaagactag
     2821 aagctttaat gagtttt
//
LOCUS       DACADF010000288         2730 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.293, whole genome shotgun sequence.
ACCESSION   DACADF010000288 DACADF010000000
VERSION     DACADF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.293"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(83..856)
                     /gene="yaiO"
                     /locus_tag="ISB30_004802"
     CDS             complement(83..856)
                     /gene="yaiO"
                     /locus_tag="ISB30_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HAP1992334.1"
                     /translation="MIKHTLLVPFFFSALPAYAGLTSITAGYDFTDYSGEHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVNAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGSSYSNMISVRLKDPRGAGYTQLWLSRGTGAYTYDWTPETRYGSMKSISLQR
                     IQPLTEQLNLGLTAGKVWFNTPTDDYNGLQLAAHLIWKF"
     gene            complement(858..1439)
                     /locus_tag="ISB30_004803"
     CDS             complement(858..1439)
                     /locus_tag="ISB30_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP1992335.1"
                     /translation="MKNYLKLNAKEKNVQIADQVIIDESTGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGKRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPSGTYSLRQELIRTGD"
     gene            complement(1417..2613)
                     /locus_tag="ISB30_004804"
     CDS             complement(1417..2613)
                     /locus_tag="ISB30_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP1992336.1"
                     /translation="MKTLLFIFMTIAMLLWFLSTLRRKPCQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     HGLKYATCDQVFLSDADTFVPPDNDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQFLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
BASE COUNT          761 a          668 c          609 g          692 t
ORIGIN      
        1 attaattata ttgtttatta tcaaaccgag ttatagcttt tctctgaaaa ctaccgaata
       61 aaatatactg atagactgct gcctagaatt tccagatcag gtgggctgca agttgcagac
      121 cgttataatc atcggttggg gtgttgaacc acactttacc tgccgtcaac ccgagattaa
      181 gttgctcagt cagcggttga attcgttgca aactgatgct cttcatgctg ccgtagcgtg
      241 tttctggcgt ccagtcgtag gtgtaagcac ctgttccgcg gcttagccat agttgcgtat
      301 aaccagcacc gcgcgggtcc ttcagacgca cggaaatcat attgctataa ctactacctg
      361 catcgctgga gtcataatgg gtatagcggt aactggtgat caccgggcca gtatagagtg
      421 atacgccgcc ttgccaggca ttgacttcga catcatcgta atatttagtg tagcgataac
      481 cggtcgtgaa aagcgttttt ggcaacaggg ctaggttaat atcctgtcga aaatcctggc
      541 gggcaaagac cggcgtatta tccgcaaagg caatacccgt tcgggttgtc agccagttat
      601 tccatttgta ccagactgca ccctggccgc gagtggcatt gaaatgttca gtttcataat
      661 cacgacgccc ctgggaaaga ttaaaaagca gcgttgcgtt ttcaactttt gctaccagtt
      721 cagcatacgc taaattacgg ttgccatgtt cgccagaata atcggtaaaa tcgtagcccg
      781 cggtaatgga agttaacccg gcataagcgg gcaatgcact aaaaaaaaac ggcactaata
      841 acgtatgctt tatcatttta atctcctgta cggataagtt cttgtcggag tgagtaagtt
      901 ccactaggta aattacgttc tacaatcacg cgcgggccaa gttgtgtgtt cggagaaata
      961 attcgcccag gcaaaataat aacttgtacg ccaaggcgtg aacgcttccc gatataacaa
     1021 cctaatttat cgcatccggt agcgataatt ccctctggag ttcgaacaga cacgggttgt
     1081 tcatccagac gatgattact ggtacgtact tgtgcgccca aatatgcctg gtttgcaact
     1141 accgagtcgg caataaagca ttgcggacca atcgttgctt ccgcttcaat aaccgcattt
     1201 ttaatttcgg tggcaaaacc aatttttacg ccattgctga ttattgtgcc aggacgaata
     1261 aacgcataat tacctatcag gcagtttgcg ccaatcacta ccggcccctg aataacggca
     1321 ccgtggcaaa tacgggtatt cgcgccgata acaacctcac cggtactttc atcaataatc
     1381 acctggtctg caatctgaac atttttctct ttagcattaa gcttcagata gtttttcagt
     1441 tcctgtgaca gaaggttggg aataagcggt tgacgcttcc accagtgcgg aatagcgggt
     1501 gggtttgtcg cgctgaggtt cgcgtccagt aaaaaaggca ataagtccat aaataatcca
     1561 gatggcataa gctaataata catacacaac ggaaagcggt gctaaaggca ccaacaacca
     1621 gcaacgatga aaccaggcgc taaatgcacc aataacacaa actacgccga cccagataag
     1681 cgggaacatt gccaacacca caccatgtgg cccggtggtg atgaattcat taaaccaggt
     1741 agtgagataa ataccaacgc cataaagcac aaccaacagc ataggaaata tactgaagat
     1801 accgaagcgg ctaaataaaa gtcttttatg caggcgcata cagaccgcgt agcccacaat
     1861 ccaacgccgc cagcggcgcc actcctcacg cggactgtta cattcctgtg ggtatacgat
     1921 gcagcgattc gcctgccgaa tacggtagcc gtttgccacc aacgtccagg tgagatcaag
     1981 gtcttcgact ttagtacgat cagagaaacc gaacttacgc aatacatcag tacggaacat
     2041 cccgcaggca ccgctgataa taaacggtgc accgcccaga aactgctgta gcgtgcgctt
     2101 cataacaatc atcggcaact ttacggtggc gcggatatgt ggcagcagac ctgccccttt
     2161 taacgcggtg gagggaattc cgccaacggc atcagcgccg cgctcaattt ctgccagcat
     2221 atagcccatt ccatcattat caggtggaac gaaagtatct gcatcactta aaaatacttg
     2281 atcacacgtg gcgtatttta gtccatgcat tagtgcgcca cctttaccgg tgtttttttg
     2341 cgtgacggca acaaagcggt cgccccattt gcgtttgact tccgccatga ccgcttcggt
     2401 attgtccgtg gatccgtcgt taacgcaaat tacccggcaa aaataagggt tacgcagcag
     2461 attatccagt gactgcgcca gacacgggcc ttcgttataa gctggtataa tggcgtcaat
     2521 acagcctttc ttttgacagg gtttacgtct tagcgtactt aaaaaccata gcaacattgc
     2581 tatggtcatg aagataaata acaaagtttt catttctatt actccgttac aacaccatct
     2641 tatggacaac aaaaccttca cacatagaat gactaacctg ttgtcctcgg aatttggctt
     2701 gagcaattat gtattctggc tgtatgtatc
//
LOCUS       DACADF010000289         2594 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.294, whole genome shotgun sequence.
ACCESSION   DACADF010000289 DACADF010000000
VERSION     DACADF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.294"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            102..299
                     /gene="fimB"
                     /locus_tag="ISB30_004805"
                     /pseudo
     CDS             102..299
                     /gene="fimB"
                     /locus_tag="ISB30_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
     gene            complement(330..1550)
                     /locus_tag="ISB30_004806"
     CDS             complement(330..1550)
                     /locus_tag="ISB30_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc53 family transposase"
                     /protein_id="HAP1992337.1"
                     /translation="MDEKSLYAHILNLTAPWQVKSLTLDENAGSVTVTVGIAENTQLT
                     CPTCRKSCSVHDHRHRKWRHLDTCQFMTLVEADVPRVMCPEHGCQTLPVPWAGSGSRY
                     TLLFESFVLSWLKISTVDAVRKQLKLSWNAVDGIMTRAVKRGLSRIKKPLSVRHMNVD
                     EVAFKKGHRYITVVSDRDGRALALTDDRGTESLASYLRSLTDSQLLAIKTLSMDMNAG
                     YIRAARIHLPNAVEKIAFDRFHVAKQLGEVVDKTRQNEHPHLPVESRRQAKGTRFLWQ
                     YSDKWMTESRQEKLMWLREQMQQTSQCWTLKELAKNIWDRPWSTERRNDWLQWISLAS
                     ECDVPMMKNAAKTIKKRLYGILNAMRHRVSNGNAEALNSKIRLLRIKARGYRNRERFK
                     LGVMFHYGKLNMAY"
     gene            complement(1569..2087)
                     /locus_tag="ISB30_004807"
     CDS             complement(1569..2087)
                     /locus_tag="ISB30_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP1992338.1"
                     /translation="MSVPQTKAELLLAIDKNFSKLISYLNTIPPEITSDKSMDGHAKG
                     TEMSVRDLVSYLLGWNALVVKWIASDAKGLPVDFPETGYKWNQLGLLAQKFYSDYSEL
                     SYELLVAELQTVKNEIVNLINDRTDDILYGRPWYTKWTMGRMISFNTSSPYANANGRL
                     RKWAKNNNISLK"
     gene            2187..2594
                     /gene="fimB"
                     /locus_tag="ISB30_004808"
                     /pseudo
     CDS             2187..2594
                     /gene="fimB"
                     /locus_tag="ISB30_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
BASE COUNT          676 a          604 c          569 g          745 t
ORIGIN      
        1 taagcaaacg ttcgctttta taaacataat caggataaaa atgttggatt attgctaacc
       61 cagcacagct agtgcgcgtc tgtaattcta agggaaaaac gatgaagaat aaggctgata
      121 acaaaaaaag gaacttcctg acccatagtg aaatcgaatc actccttaaa gcagcaaata
      181 ccgggcctca tgcagcacgt aattattgtc tgactttgct ttgttttatt catggtttcc
      241 gggcgagtga aatttgtcga ttgaggattt cggatattga tcttaaggca aagtgtatat
      301 agggtcttcc ccgatcatgg tgggaagact cagtacgcca tattcagttt cccatagtgg
      361 aacataactc ccagtttaaa gcgttcccgg tttcggtatc ccctggcctt tatcctcagc
      421 agtctgatct tgctgttcag cgcctccgca tttccattcg agacgcgatg acgcattgca
      481 ttcagtattc cgtataaccg ttttttaatg gttttcgctg cgtttttcat catcggcaca
      541 tcacattcag acgccagcga tatccactgc aaccagtcat tcctgcgttc tgtgctccag
      601 gggcgatccc agatattttt tgccagctct ttcagtgtcc agcactggct tgtctgttgc
      661 atctgttccc gcaaccacat cagcttttcc tgccgggact cagtcatcca tttgtcgctg
      721 tactgccaca ggaagcgggt acctttggcc tgacgacgac tttcaacagg gaggtgcgga
      781 tgttcattct ggcgggtttt atcaaccact tcgcccagtt gcttcgccac atggaagcga
      841 tcgaaggcga ttttctcgac cgcattgggt aaatggatac gcgctgctct tatatagccc
      901 gcattcatgt ccatcgacag tgttttgatg gccaacaact gactgtcagt aagcgaacgg
      961 agatagctgg caagactctc tgtgccacga tcatcggtta atgccagtgc ccgcccgtcg
     1021 cgatcagata ccacggttat atacctatgc ccttttttaa aggcgacttc gtctacattc
     1081 atatgacgca ctgataaagg ctttttaatc cgcgacaggc ctcgcttaac cgcgcgggtc
     1141 atgatgccgt caacggcatt ccagctaagt ttaagttgtt ttctgaccgc atcaacggtg
     1201 ctgattttaa gccatgagag cacgaacgat tcgaacagca gagtgtaccg actgccggaa
     1261 cctgcccacg gtacaggcag agtctggcag ccatgctccg ggcacataac gcggggaaca
     1321 tcggcttcta ctaatgtcat gaactggcag gtatcaaggt ggcgccattt acgatgtcgg
     1381 tgatcgtgaa cagaacagga tttcctgcag gtcggacagg ttaactgagt attttcagca
     1441 attccgactg taacagtaac ggaacctgca ttttcatcga gggtaaggga tttaacctgc
     1501 catggtgcag tcaggttaag gatatgggca tagagggatt tttcgtccat ataatttcat
     1561 tctgaagctt actttaaact gatattatta ttttttgccc actttcttaa tcttccatta
     1621 gcgttggcgt aaggcgaaga tgtgttaaat gagatcattc tccccatcgt ccattttgtg
     1681 taccatggtc ttccatacaa aatatcatcg gtacgatcat taataaggtt cacaatctca
     1741 ttttttacag tctgaagttc agctactaac aactcataac ttaactcact gtaatctgag
     1801 taaaattttt gagcaagaag gccaagctga ttccatttat agccagtttc cggaaaatcg
     1861 acaggcagac ctttagcatc agaagcgatc cactttacaa caagagcatt ccatccaagc
     1921 agatacgaaa cgagatcacg aacactcatc tccgttcctt tggcgtgtcc gtccattgat
     1981 ttatctgaag taatttctgg tgggattgtg ttgaggtaac taattaattt actaaaattt
     2041 ttatcaatag ctaaaagcag ttcagctttt gtttgcggca cactcataag aattccctct
     2101 catgtaaaaa gagacaccat aaaggtactg tgtggtgttt gaaaatcact caattatgta
     2161 ccacccacca caacagggga agacccatat atatccatcg attaaaaaaa ggcttttcaa
     2221 caacgcatcc gctattgaac aaagaagttc aggctttaaa aaactggttg agtatccgta
     2281 cttcgtaccc gcatgctgag agcgagtggg tatttttatc tcgtaaggga aatccgcttt
     2341 ctcggcaaca gttttaccat attatctcga cttccggtgg taatgccggg ttgtcactgg
     2401 agattcatcc gcacatgtta cgccattcgt gtggttttgc tttggcgaat atgggaatag
     2461 atacgcgact tatccaggat tatcttgggc atcgcaatat tcgtcatact gtctggtata
     2521 ccgccagcaa tgcagggcgt ttttacggca tctgggatag agccagagga cgacagcgtc
     2581 acgctgtttt atag
//
LOCUS       DACADF010000290         2582 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.295, whole genome shotgun sequence.
ACCESSION   DACADF010000290 DACADF010000000
VERSION     DACADF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.295"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..1667
                     /gene="evgS"
                     /locus_tag="ISB30_004809"
     CDS             <1..1667
                     /gene="evgS"
                     /locus_tag="ISB30_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAP1992339.1"
                     /translation="ENRKIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYICGWQD
                     ITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGSGLSKE
                     QRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHSFAAIA
                     ASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLGHIDDN
                     HAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNMQGDLS
                     LESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRLLLKRQ
                     LNLLGYDVDEATDGVQALHKISMQHYDLLITDVNMPNMDGFELTRKLREQNSSLPIWG
                     LTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDIEALKN
                     NTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANILNLQKL
                     INISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(1723..>2582)
                     /gene="yfdE"
                     /locus_tag="ISB30_004810"
     CDS             complement(1723..>2582)
                     /gene="yfdE"
                     /locus_tag="ISB30_004810"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAP1992340.1"
                     /translation="LAENFRPGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDA
                     PAYDTIIQAMSGIMMETGYPDAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGA
                     HVDIAMFDATLSFLEHGLMAYIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGND
                     KLFSALCQALELTELVNDPRFSSNILRVHNQAILKEYIERTLKTQVAEVWLARIHEVG
                     VPVAPLLSVAEAINLPQTQARNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHG
                     EQIRQEFSS"
BASE COUNT          769 a          599 c          544 g          670 t
ORIGIN      
        1 aagaaaaccg aaaaatatac acacaggtat ttgaaattga taatggcatc gagaaaagat
       61 gcattaatca ctggcatacc ttatgtaatc tgccagcaag cgaacatgct gtttatattt
      121 gtggttggca agatattacc gagacgcgtg atttaattca tgcactcgaa gtagagagaa
      181 ataaagcgat caatgcaact gtcgcaaaaa gccagtttct ggcaacaatg agtcacgaaa
      241 taagaacacc aataagctcc attatgggct tcctggaact tctgtcgggt tctggtctta
      301 gcaaggaaca acgggtggag gcgatttcac ttgcctacgc caccggacaa tcactcctcg
      361 gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc gggtaactat caacttcaac
      421 cacaatgggt cgatatccct actttagtcc agaacacttg tcactctttc gctgcgattg
      481 ctgcaagcaa atcgatcgca ttaagttgca gcagtacgct tcctgaacgc tacctggtta
      541 agatcgaccc tcaggcgttt aagcaggtct tatctaattt actgagtaat gctctcaaat
      601 ttactaccga gggggcagta aaaattacga cctccctggg tcacattgat gacaaccacg
      661 ctgttatcaa aatgacgatt atggattctg gaagtggatt atcgcaggaa gaacaacaac
      721 aactgtttaa acgctatagc caaacaagtg caggtcgtca gcaaacaggt tctggtttag
      781 gcttaatgat ctgcaaagaa ttaattaaaa acatgcaggg cgatttgtca ttagaaagtc
      841 atccaggcat aggaacgata tttacgatca caatcccggt agaaattacc caacaagtgg
      901 cggctgtcga ggcaaaagca gaacaaccta taacactacc tgaaaagttg agcatattaa
      961 tcgcggatga tcatccgacc aacaggctat tactcaaacg ccagctaaat ctattaggat
     1021 atgatgttga tgaagccact gatggtgtgc aagcgctaca caaaatcagt atgcaacatt
     1081 atgatctgct tattactgac gttaatatgc cgaatatgga tggttttgag ttgactcgca
     1141 aactccgtga gcaaaattct tccttaccca tctgggggct tacagccaac gcacaggcta
     1201 acgaacgtga aaaagggtta aattgcggca tgaacttatg tttgttcaaa ccgttgactc
     1261 tggatgtact gaaaacacat ttaagtcagt tacaccaggt tgcgcatatt gcacctcagt
     1321 atcgccacct tgatatcgag gccctgaaga ataatacggc gaatgatcta caactgatgc
     1381 aggagattct catgactttc cagcatgaaa cgcataaaga tctacccgct gcgtttcatg
     1441 cactagaagc tggcgataac agaactttcc atcagtgtat tcatcgcatc cacggtgcgg
     1501 ctaacatcct gaatttgcaa aagttgatta atattagcca ccagttagaa ataacacctg
     1561 tttcagatga cagtaagcct gaaattcttc agttgctgaa ctctgtaaaa gaacacattg
     1621 cagagctgga ccaggagatt actgttttct gtcagcaaaa tgactaaata gcggctccca
     1681 caatgttcaa atgtgggagc tatttaccag cacatcttac atttatgatg agaactcctg
     1741 gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc ggcataacat gcgggtccgc
     1801 gcagccgctg atttttatcg gattacccgg catcattatt cccccggctt caatcaacat
     1861 atttctcgcc tgagtttgtg gtaaattaat ggcctcagcc acacttaata acggcgcgac
     1921 gggcacacca acttcatgta ttctggctaa ccaaacttca gctacctgcg tttttaacgt
     1981 ccgctcaata tattctttaa gaatagcctg gttatgtacg cgtaaaatat tgctgctaaa
     2041 tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg cataacgcag aaaaaagctt
     2101 gtcattacca caacaaatcg taatcggctt atcctgagta tcgaaaacat caaaaggtgc
     2161 catgtaggga tggcgatttc ccaggcgttg tggtgacttc ccagtcgcga tatatgccat
     2221 cagtccatgc tccagaaaac tcagagtggc atcaaacatc gctatatcga catgcgcccc
     2281 tctctggctc ttttcgcggc cataaagtgc actcactatt ccactgaata aataaacacc
     2341 gccacagaga tccgcaagag aggtaccaac gcgcactggc ggagcatcag ggtatcctgt
     2401 ttccatcatt atcccgctca ttgcctgaat gatggtatcg taggcaggag catcttttag
     2461 cggaccggta tgtccgaaac ctgacgatga agcatatata aggcgcgggt tgatttcttg
     2521 taacctttcc catgaaaacc cgagtttttc cattgtacct gggcgaaaat tctcagctaa
     2581 ta
//
LOCUS       DACADF010000291         2578 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.296, whole genome shotgun sequence.
ACCESSION   DACADF010000291 DACADF010000000
VERSION     DACADF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.296"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            94..2373
                     /gene="ydeP"
                     /locus_tag="ISB30_004811"
     CDS             94..2373
                     /gene="ydeP"
                     /locus_tag="ISB30_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAP1992341.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMVHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          680 a          611 c          639 g          648 t
ORIGIN      
        1 ttatatatcc atgttgacga taaaccctat gtatatattg tgcatcatcg ctattacaaa
       61 tcctaataat tcatttccac acaggataag cagatgaaga aaaaaattga atcctaccag
      121 ggagctgccg gtggttgggg tgctgttaaa tccgtagcga atgcagtacg taagcagatg
      181 gatatacgcc aggatgttat tgccatgttt gacatgaata agccagaggg ctttgactgt
      241 ccgggatgtg catggccaga tcctaagcac agtgcgtcat tcgacatttg tgaaaacggc
      301 gcaaaagcaa tcgcctggga agtcacggat aagcaggtaa atgcctcttt ctttgctgag
      361 aatacggttc aatcattact tacctgggga gatcacgagc ttgaggctgc gggacgtctc
      421 actcagcctt tgaaatatga tgacgtcagc gactgttaca agccattaag ctggcaacaa
      481 gctttcgacg aaattggcgc tcgccttcaa agctatagtg atcccaatca ggttgaattc
      541 tatacttcgg ggcgcacttc caatgaagct gcctttcttt atcagctttt tgcccgtgaa
      601 tacgggagca ataactttcc cgactgctcc aacatgtgcc atgaaccgac cagtgtggga
      661 ttggcagcga gtatcggtgt gggtaaaggg accgtgttgc tggaagactt tgagaaatgc
      721 gatttagtca tttgcattgg gcataaccct ggtacaaacc accctcgcat gctgacttcg
      781 ttgcgcgctt tagtgaaacg gggagcgaaa atgatcgcta tcaaccctct acaggaacgt
      841 ggcctggagc gatttaccgc accgcaaaac ccgtttgaaa tgctgacgaa ctctgagact
      901 cagttagcca gtgcctacta taacgtgcgc attggtggcg atatggcgtt gctcaagggg
      961 atgatgcgcc tgttaattga gcgcgatgat gctgcaagcg ccgcaggtcg gccctcattg
     1021 ctggatgacg aatttattca aacgcatacc gtcggctttg acgagctacg ccgtgacgtg
     1081 ctcaattccg agtggaaaga tatcgaacgt atttctggac taagtcagac acaaatcgcc
     1141 gaacttgctg atgcatatgc cgctgccgaa cgaaccatta tctgttacgg aatggggatc
     1201 actcagcacg aacatggtac ccagaacgta cagcaactgg tcaatctgct gttgatgaaa
     1261 ggtaacattg gcaagcctgg tgcgggtatc tgcccactac gaggtcactc taatgtacag
     1321 ggcgaccgaa ccgtcggtat caccgagaaa ccgtctgcag agtttctggc tcgcctgggt
     1381 gagcgctatg gcttcacccc acctcatgca cctggacatg ctgcaatagc cagcatgcaa
     1441 gctatatgta cggggcaagc tcgagcattg atctgcatgg ggggcaactt tgcactggca
     1501 atgccagatc gggaagcgag cgctgtaccg ttaacgcaat tagatctggc ggtacacgta
     1561 gccactaagc ttaaccgctc tcatctgctg accgcacggc atagctatat tctgccggtt
     1621 ctgggacgta gcgagattga catgcaaaaa agcggtgcgc aggcggtaac cgttgaggat
     1681 tcaatgtcga tggttcatgc ctcgcgtggt gtgttaaaac ccgccggtgt aatgctgaaa
     1741 tcagagtgtg cagtggtcgc gggaatcgcg caggcagcac taccccagag cgtggtagcc
     1801 tgggagtatc tggtggaaga ttatgatcgc attcgcaatg acattgaagc tgtgctgcca
     1861 gagtttgccg actataacca gcgcatccgt catcctggtg gttttcacct gataaacgca
     1921 gctgctgaaa ggcgctggat gacgccgtca ggtaaggcta atttcattac cagcaaaggg
     1981 ctgttagaag atccttcttc agcgtttaac agtaagctgg tcatggcgac agtacgcagc
     2041 cacgatcagt acaacacgac gatttatggt atggatgatc gctatcgagg tgtattcggt
     2101 caacgagatg tggtctttat gagtgctaaa caagctaaaa tttgccgtgt aaaaaacggc
     2161 gaaagagtta atcttattgc actcacgcca gacggtaaac gtagctcacg ccgcatggat
     2221 agattaaaag tggtcattta ccctatggct gaccgctcac tggtgaccta tttcccagaa
     2281 tcgaatcaca tgctaacact tgataaccac gatccattaa gtggcattcc tggctataaa
     2341 agtattccgg ttgaattaga accatcaaat taattctcct cctcatttct tctgctgtca
     2401 tccgcacagc agaagaattc ctcattgact attatcgcgc aatttgctca catggattaa
     2461 attaaactac acactataag atataaacct ctgcctacag ttgtaagaaa ctccgctcag
     2521 tactgaagtg ccagtcctat ttcctctttt atccagcctg ttatattaat catactga
//
LOCUS       DACADF010000292         2562 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.297, whole genome shotgun sequence.
ACCESSION   DACADF010000292 DACADF010000000
VERSION     DACADF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.297"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..2440)
                     /gene="vgrG"
                     /locus_tag="ISB30_004812"
     CDS             complement(<1..2440)
                     /gene="vgrG"
                     /locus_tag="ISB30_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAP1992342.1"
                     /translation="MDIPTLNFDHSHHKLKIRGLKSPADVLTFTGHEQLSSPFRYDIE
                     FTSTNKAITPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITSFKHLSSSQDEARY
                     EVRLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKNEYPAREQV
                     MQYGEDDLTFVSRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGL
                     FDGETEAVWGLNSAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYA
                     DNFLQKGDKEAAESGAFYARIRHERYLNEQAILQGQSTSSLLMPGLEIKVQGDDAPAV
                     FRKGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPCPVMAGTLPARVTSTVK
                     NDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSI
                     AFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVST
                     EYGGKSQLNLGHLVDAGKQQRGEGFELRTDLWGTVRAKKGIFISSDAQDKAQGKVREM
                     APAMAILDGAQSQMKSLSTDAQTANADPADLSSQIALLQQSVKDLTQAAILLSAPKGV
                     AIASGEHLQLAASKNLIANAGNHADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAI
                     SVQAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEY
                     NVKAGYYGRKPKAKLTPELMAFPVIKSEDFNQSFILLDENTGQPLINWPYELELESGL
                     KMSGITDENGNTELI"
BASE COUNT          492 a          728 c          676 g          666 t
ORIGIN      
        1 atattagttc tgtatttcca ttttcatcag taatcccact catttttagc ccactttcca
       61 gttctagttc atagggccaa ttaataagtg gttgcccagt attttcatcc agcaaaataa
      121 agctctgatt aaaatcctca gacttaatca ccggaaatgc catcagctcc ggcgtcagct
      181 tcgccttcgg cttacgcccg taatatcccg ccttaacgtt atattctccc ggcgtaccgg
      241 cctctatccc acaggcatcc agccggatgt agctgccacc gccgttaagg gtgattttct
      301 tcttcgcggt gattttaatc tcatcttccg tactggtgat ctctatcgat ttctgcgcca
      361 acagttccat cagatcgttc tgtgcctgta ccgagatcgc ccctttattg gcaaacagtt
      421 tgatacccgc tttgcgtaca aacacgctca gcgcctgacc cacgccgata aacatattct
      481 tcacgacacc gatatcagcg tgatttcctg cgttcgcgat caggtttttc gacgccgcca
      541 gttgcaggtg ctctccgctg gcgatcgcca cgcctttggg tgcgcttagc aggatcgcag
      601 cctgcgtcag atcttttaca ctctgctgca acagcgctat ctgtgatgac agatctgccg
      661 gatcggcatt cgccgtctgc gcatcagtgg acagcgactt catctgtgac tgtgcgccat
      721 ccagtattgc catcgccgga gccatttcgc gcaccttacc ctgcgccttg tcctgcgcgt
      781 cggagctgat aaagatgcct ttctttgccc gtactgtacc ccacagatcg gtacgcagtt
      841 caaagccttc cccgcgctgc tgttttccgg catccaccag atggccgagg ttgagctggc
      901 tcttgccgcc atactcggta ctgaccttga tatgctcttt gccgcgctcg tcatcgaggc
      961 ggattttgtt gttcgccggg gtgcgcagta cgttgcgctt gtagttctga atggtcacat
     1021 ggtcggtgtg tgccgagtcg tgttttacgc ctgcaatata aggtcggtcc gggttgcctt
     1081 cctcaaacgc gatgctgact tccgttcccg ccagcagcgg cagatgcagg ccgtaggtgt
     1141 ctcccgcata cgggcgcgac tggcgtaccc acaggctttc ataacccggt ttccacgtat
     1201 cgcggtcaaa gtcgaggttg acccggtagc gcccgtcctt gtcgatatgg gcgtagatgt
     1261 cgttcttcac cgtgctggtg acccgtgccg ggagcgtgcc ggccatcacg gggcagggga
     1321 taagcgccgg gcggtagcca tagcgttctg agtacgggat ggcggtaaac gtcagttcat
     1381 agctgcggtc acgcgccgcg ctggccgtca cgccggtaat cagtacgccc ttgcggaaca
     1441 ccgctggcgc gtcgtcaccc tgaactttta tctccagccc cggcatcagc agtgaactgg
     1501 tgctctggcc ctgtagaatg gcctgttcat tcaaatagcg ttcgtggcgg atacgggcat
     1561 aaaacgcgcc gctttccgct gcctctttat cgcctttttg caggaagttg tcagcgtagt
     1621 ggtaggcttc gccgtaggtg gtgttatcgc ccccggtggc gtcatgctgg cctgtggtca
     1681 tttccgccgt ggcttcacgg tagttgtagt cccgcgtgga gacgcttttt tccaccacgc
     1741 tgtacgcgct gttcaacccc catacggctt ccgtttcacc atcaaacagc cctgaaggat
     1801 gacgaagcgg cagcgtcagt ccgcgctcat agccgctctg gtcgtcataa aactcgacaa
     1861 cttcgatttt cagccgggcg tcggtggcaa agcggaacca gatacccacc tccgacagca
     1921 ggcgtgagac aaacgtcagg tcgtcctcac cgtactgcat cacctgttca cgggccgggt
     1981 attcgttttt gaggttaaac acgaagtcct gaccgcgcat ttgatggcgc tcgcgcagga
     2041 ttttttcgac gatttgcggc accgtctggt tctggtagat ggcgttctgg cggctgcggg
     2101 taagcagcgc catacggggt tcaaggcgca cttcgtaacg ggcttcgtcc tgtgacgatg
     2161 agaggtgttt aaagctggta atgacgccgt gcagggtgcg cagggcagtc tgcaccggca
     2221 ttccctgcac gggcggtgcc gtcaggctga atgcgccgtc ctgcatcagc accgactccg
     2281 gcgttattgc tttattggta ctggtaaact caatgtcgta gcggaagggt gagctgagct
     2341 gttcgtggcc cgtaaacgtc agcacatccg ccgggctttt caggccgcga attttgagct
     2401 tgtggtgtga gtggtcgaaa ttcagagtcg ggatatccat ctctgtactc ctttatattt
     2461 atgaaaaata aatccaagta tgattctctt taaaaaggga aaaatgcaaa aaatataact
     2521 aattaaagta agctacttat aatttctaat catccctgcg ag
//
LOCUS       DACADF010000293         2547 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.298, whole genome shotgun sequence.
ACCESSION   DACADF010000293 DACADF010000000
VERSION     DACADF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.298"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            101..1033
                     /locus_tag="ISB30_004813"
     CDS             101..1033
                     /locus_tag="ISB30_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP1992343.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVRGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(1074..2504)
                     /locus_tag="ISB30_004814"
                     /pseudo
     CDS             complement(1074..2504)
                     /locus_tag="ISB30_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
BASE COUNT          661 a          558 c          532 g          796 t
ORIGIN      
        1 atgtggatag atatgaatta tttttctcct taaggatcat ccgttatttg ggtcgttttc
       61 tgctaaaatc tgcgccttgc gcttctttag cgcactgtct atggctaacc ttttgaataa
      121 attcattatg acgagaatac tcgctgcgat aacccttttg ctgagtatcg tattgaccat
      181 tttggtcact attttttgct ccgtcccgat catcattgcc gggattgtaa aactattgct
      241 tcctgtgcca gtcatttggc gaaaggtttc gcgtttttgt gattttatga tgtattgctg
      301 gtgtgaaggt ctggcggtat tactgcatct gaacccacac ctgcaatggg aagttcgcgg
      361 gctggaaggg ttaagtaaga aaaactggta tctgcttatt tgtaaccacc gtagttgggc
      421 agatattgtc gtactgtgcg tgctgtttcg taagcacatt ccgatgaata aatatttcct
      481 caagcagcag ctggcctggg tgccttttct tggcctggca tgctgggcgc tggatatgcc
      541 gtttatgaag cgttattccc gcgcttattt attacgtcat ccggagagac gtggtaaaga
      601 tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg catcccacca ccattgttaa
      661 tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag caaacgcact ccacttttca
      721 aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg ctaaatgtac tgggtaaaca
      781 attcgataaa ctgcttaatg ttacgctgtg ttatccggac aataaccgtc agccgttctt
      841 cgatatgtta agcggtaaac tgacgcgaat tgtcgtacat gtggatttgc agcctattgc
      901 cgatgagtta catggcgact acatcaatga caaaagcttt aagcgccatt ttcagcaatg
      961 gctgaactca ctgtggcagg aaaaagaccg gcttctgacg tcgttgatgt catcacagcg
     1021 gcaggacaag taaattttgc tcgcctctga atgagcagag gcgagtgagt attttaatga
     1081 acaaaacgtc cggcacgaga cataaattct tcttcgctca tctcctgtcc gttaaaaaca
     1141 actttacccg gtgtataacg cagttgcagc gaggcggtat tgttgtcaat cgtgattaac
     1201 tgcaacatct gacctaaggt catcatcccg ctgatttgtt tatcagactg cttttgcgct
     1261 ttttccgcat ccattccttc agataaatta agctgttttg cggtttctgt ggcgacatta
     1321 agcggtaatt tcacatcaaa attgagcgat ttaatatctt tgtttgtgga tgatgaagat
     1381 ttggctgggt cggcaatact gatatccaga ttggcattta gttcgccgag tgcattcttc
     1441 catcttaccg gttgtttaat ggtcggctca cttttttgta ataacggtaa atactctttg
     1501 aacaatgcag tattcacttc ttgcagagca acttcatcgt ttgctaactc agggtgtgca
     1561 gcaagctgct tctgcatcgc aatgttatac tggatgataa attggcgcac cgctgaggga
     1621 tcaatagatt cagcaatcaa tgaccactca ccactaccga gttgctgatt cccttttgtt
     1681 aacttatcaa gttcgtaggt cagattgaca ttgatataat ctttgtcttt aacgcgatcc
     1741 agcttggttt ttgcaacgaa agcatcgatt tgggcaacgt cttccccgtg attgataatg
     1801 ttaatctgat taaatttcga ctcgctttca ccaaccggaa atttcttttc ttctaatcgc
     1861 gccagagaat caatggtaaa acttttcgcc gttactttgt tcattggtga taattgcaga
     1921 acaatatctt caactttgcc ttcaatatcc aggttagaca atgaactatc atcaccatta
     1981 aaagtaaact ggccttcacc aaaagaaaac tttccatact ctgcaggggc aacatctaat
     2041 gtcgtggaga atgagccacc aaacgcgaat cgcaatgtcg cttccacggg cgatttattt
     2101 ttcgcggcga taaatagcgg ttgtgtcagc tcattattca ctaagttcac ttttgccgct
     2161 gccagtgctg ggatgacatt accatgcatt aacattgtga tgggcaacgg accgtgctca
     2221 acatccacat caaatacaac tttctggcct ggcttgatat tgagatcggg tgcaccgtta
     2281 tcaaatgtca tttgcatttg aaaacgggaa ttaaaaaaac ctttgtcaaa atttttataa
     2341 cttaacgtca tgtcgtaggc atgttcacct ttctatttag catcgttaaa atctttaata
     2401 aacttttcga cacctggctg aatttgcgta cctgtgtacc atgtgccacc accccagatt
     2461 acggataacg caacaatggc acctgtagct gacttacgta tcatataaat attccctgat
     2521 aaaacagcaa aactttgaat atccact
//
LOCUS       DACADF010000294         2505 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.299, whole genome shotgun sequence.
ACCESSION   DACADF010000294 DACADF010000000
VERSION     DACADF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.299"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..915
                     /locus_tag="ISB30_004815"
     CDS             <1..915
                     /locus_tag="ISB30_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HAP1992344.1"
                     /translation="DHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQL
                     NLGHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQVREMADIISELN
                     SLSDKIQKLSDDAATANADPADMAAQVALITSRINDLTASVILMHAPKGVAVASGEHL
                     QLAAVKNLQINAGNNADIGVVKNMFIGVGRALSVFVRKAGIKLIANKGAVSVQAQHDL
                     MELLAKKSIEIVSTEDEIRISAKKKITINGGGSYIRIEGSGIEPGTPGDYNVKAVHYG
                     RMGKAHEPVELQMLAEKVDEPPVKFFFS"
     gene            927..>2505
                     /locus_tag="ISB30_004816"
     CDS             927..>2505
                     /locus_tag="ISB30_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HAP1992345.1"
                     /translation="MIISPPFIRAKNNNENDAHWIERMMPVEPDRDYPINYGGSWHGG
                     IHVRHTNSDRQPEYVRAIADGVVVSIRNPSDQAACSLPPLNYNGSTDDGYVLLRHETE
                     IGTGEDGNIVYYSLYMHLEKIEEGISEGNKVYRKSSLGMPGHVDGDRGLHFQIFCDDD
                     NLRKITGRTTPEMDVSRHGRTDVVYGDIYFYLPPGTRFYRSAPENNAVDNTGEVFQST
                     EPLVVTMCFDTGHCIMTTRRANPVIPSRYDIVGEVLTDADGTDYEYNLYSAALRLYPQ
                     SPSAGFELLRFGRVINTEHETLIPANAPLWRTVSYPGGQGVINLAADVVTKFSDGDFP
                     HWSGWRLVDDDADSNSQCNSVILRGGGFTDFRQMICHFPLEWCNTGIEARYGWLKEGV
                     IQERQENEANSDLSLGTDYYRPNNVEYNTTGDSHGESPQSAISEMPDLSYEQSNAYEN
                     AVRVLLLGEEDWQTLVKHIKALCFDTSSIAEGRVWHFNPGEFIKHFRKCGWVDKAILK
                     RIFASGTNDRDRAVIEQK"
BASE COUNT          704 a          514 c          714 g          573 t
ORIGIN      
        1 gaccatgtga ccattcagaa ctacaagcgt aacgtgctgc gcaccccggc gaacaacaaa
       61 atccgcctcg acgatgagcg cggcaaagag catatcaagg tcagtaccga gtacggcggc
      121 aagagccagc taaacctcgg ccatctggtg gatgccggaa aacagcagcg cggggaaggt
      181 tttgaactgc gtaccgatct gtggggtgca gtacgggcaa agaaaggcat ctttatcagc
      241 gccgacgcgc aggataaggc gcagggtcag gtacgcgaaa tggccgacat tatcagcgaa
      301 ctgaacagcc tgagcgataa aatccagaaa ctgtctgacg atgcggccac ggcgaacgcc
      361 gatccggcgg atatggccgc gcaggtggcg ctgataacca gccggataaa tgacctgacc
      421 gcctcggtga ttttgatgca cgcgccgaaa ggtgtggcgg tcgccagcgg cgagcacctg
      481 caactggccg cagtgaaaaa cctccagata aatgcgggaa acaacgccga tatcggcgtg
      541 gtgaagaata tgtttatcgg cgtagggcgg gcgctgagcg tgtttgtgcg caaggcgggt
      601 atcaaactga ttgccaataa aggcgcagta tcggtacagg cgcagcacga tctgatggag
      661 ttactggcga agaagtcgat agagatcgtc agtactgagg acgagatcag gatttcagcg
      721 aagaaaaaaa taacgattaa tggtggtgga agttatatcc ggattgaggg tagcggcatt
      781 gagccgggaa cgccggggga ttataacgtc aaagcggtgc attacgggcg tatgggaaaa
      841 gcacatgagc ctgttgagtt acaaatgttg gcagagaaag tagatgagcc acccgtaaaa
      901 tttttctttt cttaaggata taagaaatga ttattagccc gccatttata cgagcgaaaa
      961 ataacaatga aaacgatgct cattggattg aaaggatgat gccagttgaa cctgaccgtg
     1021 actatcccat caactatggg ggatcatggc atggaggcat ccacgttaga catacaaaca
     1081 gtgacaggca accagaatat gtccgtgcca ttgctgatgg tgtcgtggta tctattcgca
     1141 atccctctga ccaggcagca tgtagtcttc cgccgctcaa ttataatgga agcacagacg
     1201 atgggtatgt tctgcttcgt catgaaactg aaataggcac tggtgaggac ggcaatattg
     1261 tttattactc tctttacatg cacctggaaa agattgaaga gggtatcagt gaaggaaata
     1321 aagtctaccg gaaatcgtca ctgggaatgc cggggcatgt cgacggggat cgcggcctgc
     1381 acttccaaat tttctgcgat gatgacaatc tgagaaaaat aaccgggcgc actacacctg
     1441 agatggatgt gagccgtcat gggcgtaccg acgtggttta cggggatatc tatttttacc
     1501 tgccgcccgg tacccgcttt tacaggagtg cgccggaaaa caacgcagta gataacacag
     1561 gtgaagtttt tcagagcacg gaaccgttgg ttgtcaccat gtgttttgat actggccatt
     1621 gtatcatgac tacccgcaga gcaaatccgg tgataccaag ccgttatgac attgtgggag
     1681 aagtgttgac ggatgctgac ggcactgatt atgaatacaa cttgtacagt gctgcactga
     1741 gactttatcc gcaaagtcca agcgcagggt ttgagttact gcgctttggt cgggttatca
     1801 ataccgagca tgaaacactg atacccgcga atgcgccact gtggcgaacc gtgagctatc
     1861 ccggtgggca aggagtgata aatctggcgg cagatgttgt gacaaaattt agcgacggtg
     1921 attttccgca ctggagtggc tggcgactgg tagatgacga cgctgacagc aacagtcagt
     1981 gcaattcagt aatattacgt ggtggtggtt ttacggattt tcgtcagatg atttgccact
     2041 tcccactgga gtggtgtaat acgggaatag aagcgcgtta cggctggttg aaagaagggg
     2101 taatacagga acgtcaggaa aatgaggcta acagcgattt atcattaggt acggattact
     2161 acaggccaaa taatgtcgaa tataatacga ctggagatag ccatggagaa tctcctcagt
     2221 cagctatatc agaaatgcca gatttgtctt atgaacagtc aaatgcttat gaaaatgccg
     2281 tcagagtttt attactggga gaagaagact ggcagacgtt ggtgaagcat attaaggcac
     2341 tgtgttttga taccagtagt attgctgaag gacgggtttg gcattttaat ccgggtgagt
     2401 ttattaagca ttttaggaag tgtgggtggg ttgacaaagc aatattgaag cgaatttttg
     2461 cctctggtac taatgaccgt gatagagcgg ttattgaaca aaaag
//
LOCUS       DACADF010000295         2485 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.300, whole genome shotgun sequence.
ACCESSION   DACADF010000295 DACADF010000000
VERSION     DACADF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.300"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            87..698
                     /gene="ruvA"
                     /locus_tag="ISB30_004817"
     CDS             87..698
                     /gene="ruvA"
                     /locus_tag="ISB30_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP1992346.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            707..1717
                     /gene="ruvB"
                     /locus_tag="ISB30_004818"
     CDS             707..1717
                     /gene="ruvB"
                     /locus_tag="ISB30_004818"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP1992347.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MS"
     gene            1827..2485
                     /locus_tag="ISB30_004819"
                     /pseudo
     CDS             1827..2485
                     /locus_tag="ISB30_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
BASE COUNT          615 a          595 c          668 g          607 t
ORIGIN      
        1 aagttttctt catccttcgc tggatatcta tccagcattt ttttatcata cagcattatc
       61 tttgattcat tacgcaggag cgtcatgtga taggcagact cagaggcatc atcattgaaa
      121 aacaaccccc gctggtgtta attgaagtgg gcggcgtagg ctatgaagtg catatgccga
      181 tgacctgttt ttatgaactc cctgaagcgg gtcaggaagc gatcgttttc acccactttg
      241 tggtgcgtga agacgcgcaa ctgctgtacg gttttaacaa taaacaagag cgcacgttgt
      301 tcaaagagtt gatcaaaacc aacggcgtcg gcccgaagtt ggcgctggcg atcctctccg
      361 gaatgtcagc gcagcagttc gttaatgccg ttgagcgtga agaagtgggg gcactggtga
      421 aactgccggg tattggcaaa aaaaccgccg aacgtttgat tgtcgaaatg aaagaccgat
      481 ttaaaggttt gcatggcgat ctctttacgc cagccgccga cctggtactc acgtctcctg
      541 ccagcccggc gaccgacgat gctgaacagg aagcggttgc tgcgctggtg gcactgggct
      601 ataaaccaca agaagcaagc cgcatggtga gcaaaatcgc tcgccctgac gccagcagtg
      661 aaacattaat tcgcgaagcc ctacgcgccg cgttatgagg taaaggatga ttgaagcaga
      721 ccgtctgatt tctgccggta ccactttgcc ggaagatgtg gcagatcgcg ccattcgccc
      781 taaattactg gaagagtatg ttggtcagcc gcaggttcgt tcgcagatgg agattttcat
      841 caaagcagcg aaactgcgcg gcgatgccct cgatcatcta ttgatttttg gtcctccggg
      901 gttgggtaaa actacgcttg ccaatattgt cgccaatgaa atgggcgtta atttacgcac
      961 gacttctggt ccggtgctgg aaaaggcggg cgatctggca gcaatgctca ctaaccttga
     1021 accacacgat gtactgttta ttgatgagat ccaccgtctt tcaccagtgg tggaagaggt
     1081 gttgtatccg gcaatggaag attaccaact ggatatcatg attggtgaag gtccggcggc
     1141 acgctccatt aaaattgatt tgccgccgtt taccctgatt ggtgcaacca cgcgcgcagg
     1201 ttcgctgaca tcaccgttgc gcgatcgttt tggtattgtg caacgtctgg agttttatca
     1261 ggtgccggat ctgcaatata tcgtcagtcg cagcgcacgc tttatggggc ttgagatgag
     1321 tgatgacggc gcgctggaag ttgctcgtcg tgcgcgcggt acgccacgta ttgccaaccg
     1381 tctgctgcgt cgagtgcgtg atttcgccga agtgaagcac gatggcacca tctcggcaga
     1441 tatcgctgct caggcgctgg atatgttgaa tgtcgatgct gaaggtttcg attatatgga
     1501 ccgcaaattg ttgctggcgg taatcgataa gttctttggc gggccggtag gtctggataa
     1561 cctggcggca gccattggcg aagaacgtga aaccattgag gatgtactgg aaccttattt
     1621 gattcagcaa ggctttttgc agcgtacacc gcgtgggcgt atggcgacga cgcgggcgtg
     1681 gaatcacttt ggcataacgc cgccagaaat gtcgtaagtc ggattggtcg ctcgtgccgc
     1741 atccgacact ggcagtgtgc ctgatgcgac gctgttgaac tgcaccccaa atgttggaca
     1801 taatctgaca tctgaaggtg cagtttatga aacattcatt tgaagtaaaa cttgccgctg
     1861 taaatcatta cctggctggt catgcaggca tcatttctac ggcaaaactc tttcaacttt
     1921 ctcacaccag cctctcacac tggattaatc tttttcttct tcacggtcct cgggcactgg
     1981 attgcagaca caagcgtagc tattctcctg aagataaact ttgcgtggtt ctttatgctc
     2041 tcgggcattc tgagtctcta ccccgggtag cagcccggtt taacattccc agccacaaca
     2101 cggttaaaaa ctggataaaa ggctaccgca aatctggtaa tgaggccttt atcaggcgca
     2161 ggaaggagaa aagcatgacc cgttctgatg atacccatga aaacgaggca aacatgacgc
     2221 cggaagagat gaagaatgag ctgcgttatc ttcgcgcaga aaatgcatat ctgaaagcca
     2281 tgcaggagca tcttctggaa aaaaagcgcc aggagctgga gaaaaaacga aagtcatcca
     2341 gagcctgagg tacggacact gccagtctga cctgttaaag gccgcagggc tggccagaag
     2401 cacgctgtac taccagctgt cactgcagaa ggctaaggac aagtatgctg atgtgaaaca
     2461 gctcatagcc tccatcttcc atgaa
//
LOCUS       DACADF010000296         2460 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.301, whole genome shotgun sequence.
ACCESSION   DACADF010000296 DACADF010000000
VERSION     DACADF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.301"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..545)
                     /locus_tag="ISB30_004820"
     CDS             complement(<1..545)
                     /locus_tag="ISB30_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1992348.1"
                     /translation="MSTYENILRITDTLIQQRGFLGFSYADLEKEIGIRKASIHHHFP
                     RKTDLGIAYCQYKTEVFDRLNITLHNVSSGVQRLRTYLDAFAGCAERGEMCGIYAMLS
                     DSHQFSPELQEAVSQLAHQEIQMIKDIITSGQNSGEFKTVLLPDELAVIVCSTLKGAL
                     MLNRLPPHDIYSGTVNALIKML"
     gene            complement(587..847)
                     /locus_tag="ISB30_004821"
     CDS             complement(587..847)
                     /locus_tag="ISB30_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAP1992349.1"
                     /translation="MNYVICTTIVALGLILTGYATAADEINRQDVVGLMPSDYISVGN
                     LYTPEEVATALKDKAEQAGGSHFRITSLGGKNKLDGTAIIYR"
     gene            1396..2145
                     /locus_tag="ISB30_004822"
                     /pseudo
     CDS             1396..2145
                     /locus_tag="ISB30_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          685 a          529 c          586 g          660 t
ORIGIN      
        1 agcattttaa ttaaagcgtt aacggttcct gaatagatat catgtggtgg aagtcgattt
       61 aacatcagtg ccccctttag tgtagaacag actataacgg cgagctcatc cggaagaaga
      121 actgttttga actctccact attctgcccg gaggtgataa tatctttaat catctgtatc
      181 tcctgatgtg ccagttgact tacagcctcc tgtaattcag gagaaaactg atggctgtcg
      241 gatagcatgg cataaatacc gcacatttcc cctctttcag cacaaccagc aaatgcatca
      301 agatatgtcc tgagacgttg tacacctgaa gaaacattat ggagagttat attaagtcta
      361 tcaaatactt ctgttttgta ttggcagtag gctataccaa gatcagtttt cctcgggaaa
      421 tggtgatgaa tactggcttt tctgatacca atttcttttt ccagatctgc gtaactgaac
      481 cccaaaaatc cgcgttgctg gatcagagta tccgttatgc gaagtatatt ctcataagta
      541 gacacggctt tacctgttat gtattaaagg gactattata catattttag cgatagataa
      601 tggcagttcc atcaagttta ttttttcctc ccaatgaagt gattcggaaa tgagaacctc
      661 ctgcctgctc cgctttgtct ttaagcgcag ttgccacctc ttctggtgta tatagattac
      721 caacagaaat atagtctgac ggcatcaaac caacaacgtc ctgacgatta atttcatctg
      781 cagcagttgc gtagcctgtc agaatgagac caagggcgac aatggtagtg cagataacat
      841 aattcatgag caatttctct ttatgtttgc gatactacac atgctacctt ctgattggta
      901 ggcttgcaag atggatttat ttaaaataag agggatgatt tgtatcaaat tgttttgtat
      961 ttttgtgttt tgtatcactg aggtgaagtt gtaagatctg gccctctgcg aggttggctc
     1021 tgtcgaaagg aacaggtgac gagagttaac atcgttgcct tggctgcatg ggggatatgt
     1081 agctaccccc tgatatttcc gccacggaag ccaatatgaa caacaataac aatacgcctc
     1141 gtttccagta tgcttcatgt cggatatttt gtaagcgcta tgtacttccc gggacgctta
     1201 tcttttagcc gtagctggta gtgcaataaa agcctggcag caatccaggc ctctacgttc
     1261 taagaatcaa ctgtcagtgc agaggaatgc gcacgacgct ctcgttgtta ttggcttttg
     1321 ccgtcgcacc gtcgtttttc acggtgacaa acagcgcgtg cgctttgcta tccgtcttat
     1381 atcactggtg ctgacaatgt ggatgaggtc gcctttaaaa aaggacatcg ttatataacg
     1441 gtgatctccg atcgcgatgg tcgtgcgctg gccttaacgg acgatcgtgg cacagagagc
     1501 ctcgccggct atcttcgcac gcttactgac gggcagttgc tggctatcaa aacgctctca
     1561 atggacatga atgcaggcta tataagagca gcgcgtatcc acttaccctg tgcggttgag
     1621 aaaatcgcct tcgaccgctt ccatgtagca aagcaacttg gcgaagtggt tgataaaacc
     1681 cgtcagaatg aacatccgca cctccctgtt gaaagttggc gtcaggcaaa ggggacccgc
     1741 ttcctgtggc agtacagcga taaatggatg accaaatcca ggcaggaaaa gctgatatgg
     1801 ctgcgtgcac agatgaagct gacaagccag tgctgggcgc tgaaagagct ggcaaaggat
     1861 atctggaaca ggccatggag tgaggaaaga cgtaatgact gggagagatg gctggctctg
     1921 gcggccaaca gtgacgttcc tatgatgaaa aacgccgcga aaacgatagg aaaaaggctt
     1981 tacgggatcc tgaatgccat gcgacaccgt gtctcaaacg gaaacgcgga ggcgcttaac
     2041 agtaagatca ggctactgag gataaaagcc aggggatacc gaaatcggga gcgctttaag
     2101 ctgggcgtga tgttccacta cgggaaactg aatatggcgt tctgagcctt cccaccatga
     2161 tcagggaaaa cccatgtatc atcaggttat tatgtgagct ggtaaatcag attttgcatg
     2221 gactgaaatc atgctgttat ttagggataa agaaccggct ttttgtcccg ccccctcgcc
     2281 attaatgaaa aaaagaatgg aatgctccct ctgaggagaa gggcagtaga aaaaagaacc
     2341 cgccagcaaa aatattgggg atgaacagct ttcgctactc agattgctgg cgggtatagt
     2401 tcctcatgaa ataaaaatac tacataatcc ttttaatgca aatgataatc attgtcaatt
//
LOCUS       DACADF010000297         2442 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.302, whole genome shotgun sequence.
ACCESSION   DACADF010000297 DACADF010000000
VERSION     DACADF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.302"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            53..478
                     /locus_tag="ISB30_004823"
     CDS             53..478
                     /locus_tag="ISB30_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP1992350.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            475..825
                     /gene="tnpB"
                     /locus_tag="ISB30_004824"
     CDS             475..825
                     /gene="tnpB"
                     /locus_tag="ISB30_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP1992351.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            856..>2442
                     /locus_tag="ISB30_004825"
     CDS             856..>2442
                     /locus_tag="ISB30_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HAP1992352.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLP"
BASE COUNT          621 a          610 c          689 g          522 t
ORIGIN      
        1 agacgagaat tggtaactta gacgcccatc tgatatagac ggacatctaa gtatggaatt
       61 acaggactgg cgaaaagaac ctcgtaaaaa ctattcgaat gaattcaaac ttcgtatggt
      121 ggaactggca tcacaacctg gagcttgtgt tgcacagatt gcacgtgaaa atggcgtcaa
      181 tgataatgtt attttcaaat ggctcaggct ctggcagaac gaagggcgtg tttcgcggcg
      241 tcttccggta acgacctctt ctgacactgg cgttgaatta ttacctgtag aaataacgcc
      301 ggatgagcag aaagaacctg tggcggccat tgcgccgtct ttatccactt ccactcagac
      361 cagagtcagt gccagttcct gcaaggtgga attccgtcac ggtaacatga cgctggaaaa
      421 tccatcgcca gagctgctca cagtgttgat ccgtgaactg accgggaggg gaagatgatc
      481 tcactcccat caggtacccg tatctggctc gttgccggcg ttaccgatat gcgtaaatcc
      541 ttcaacggac tgggagaaca ggtacaacat gtgctgaatg ataatccctt ctccggtcac
      601 ctgtttatct tccgtggccg acggggtgac accgtcaaaa ttctttgggc tgatgctgat
      661 ggtctgtgcc tgttcaccaa acgcctggag gaaggccagt ttatctggcc tgcggtacgt
      721 gacggcaagg tatccattac ccgctcgcaa ctggcaatgc tcctcgataa gctggactgg
      781 cgtcagccaa aaacatccag ccgtaactca ctgacaatgt tgtaaaaaac tcctgaccgc
      841 attataaaaa cggtcatgag tcagaaatac ctcattcgca tcgcagagct ggaaaggttg
      901 ctctctgagc aggctgaagc cctccgtcag aaagaccagc aactgagtct ggttgaagag
      961 acggaagcct tcctgcgctc tgcactgaca cgtgccgaag aaaagatcga agaagatgaa
     1021 cgggaaatag aacatctgcg ggctcagata gaaaaactgc gccggatgct gttcggtacc
     1081 cgttctgaaa aactgcgtcg tgaagttgaa ctggctgagg ctctgctgaa acaacgtgaa
     1141 caggacagcg atcgttacag tgggcgggaa gacgatcctc aggttccccg ccagttgcga
     1201 cagtcgcgcc atcgtcgtcc gttaccggca caccttcccc gtgaaataca ccgcctggag
     1261 ccagaagaaa gctgttgccc ggagtgtggc ggtgagctgg attatctggg ggaagtcagc
     1321 gctgaacagc tggaactggt gagcagtgcc ctgaaagtga tccgcacaga acgggtaaaa
     1381 aaagcctgta caaaatgtga ctgtattgtt gaagcaccgg cgccgtcccg cccgatagag
     1441 cgtggtatcg cgggccccgg attacttgcc cgcgtgttaa cgggaaaata ctgcgaacat
     1501 ctgccactgt atcgtcagag tgaaatcttt gcccgccagg gtgtcgaact gagccgggcc
     1561 ttactctcca actgggttga cgcgtgctgc cagttaatga caccggtgaa tgatgccctg
     1621 taccgttatg taatgaacac ccgcaaggtt cacactgatg acacaccggt aaaggtactg
     1681 gcaccgggtc agaaaaaggc gaaaacaggg cgtatctgga cgtatgtccg ggatgatcgc
     1741 aatgtgggtt cgtcatctcc tccagcggtc tggttcgcgt actcgccgaa ccggcagggg
     1801 aaacacccgg agcaacacct ccgccccttc cggggtatcc tgcaggcgga tgcgttcaca
     1861 ggttacgaca ggttgttcag tgcagaacgt gaaggtggtg cactgacaga agttgcgtgc
     1921 tgggcccatg cccggcgaaa aatccacgat gtatacatca gcagcaaaag tgcgacggca
     1981 gaagaagccc tgaagcgaat cagtgaactg tacgccatcg aggatgaaat acggggatta
     2041 ccggagtcag agcgtcttgc cgtcaggcag cagcgaagca aagtgttact gacgtcgctg
     2101 catgaatgga tggtggagaa gaatggtacg ctgtcgaaaa aatccagact gggcgaagcg
     2161 ttcagctatg tactgaatca gtgggatgcc ctctgttatt acagtgatga cggtctggcg
     2221 gaggcggata ataatgctgc ggaaagagcg cttcgtgcag tctgtctcgg aaagaaaaac
     2281 tttatgttct ttggcagcga tcacggcggc gagcgtggag cactgttgta cgggctgatc
     2341 ggcacctgcc gtctgaacgg tatcgatccg gaagcgtatc tgcgccatat cctgagcgta
     2401 ctgccggaat ggccttccaa ccgagttgac gaactcctgc ca
//
LOCUS       DACADF010000298         2416 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.303, whole genome shotgun sequence.
ACCESSION   DACADF010000298 DACADF010000000
VERSION     DACADF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.303"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            271..414
                     /locus_tag="ISB30_004826"
     CDS             271..414
                     /locus_tag="ISB30_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAP1992353.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            427..636
                     /gene="cspE"
                     /locus_tag="ISB30_004827"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             427..636
                     /gene="cspE"
                     /locus_tag="ISB30_004827"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAP1992354.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            802..1611
                     /gene="rlmA"
                     /locus_tag="ISB30_004828"
     CDS             802..1611
                     /gene="rlmA"
                     /locus_tag="ISB30_004828"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAP1992355.1"
                     /translation="MSFSCPLCHQPLLREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLREWLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELVRVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1608..2174)
                     /gene="mntP"
                     /locus_tag="ISB30_004829"
     CDS             complement(1608..2174)
                     /gene="mntP"
                     /locus_tag="ISB30_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAP1992356.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          640 a          577 c          587 g          612 t
ORIGIN      
        1 cacgcactat gtaataactt ctcccactgg cctggaacaa ctgaacttat tgaactatgt
       61 tagaaaatac gtcagtttaa gtatctgcct gaactggcaa ggttaagcac aatgatatat
      121 cggcgcgtat tccgttgcat aagtgtgcaa aaaaagtgga agacgtatcg agatttgtgc
      181 gtctgatcga gacatgttta aaaatggctt gccataatta acgttgtatg tgataacaga
      241 tttcgggtta aacgaggtac agttctgttt atgtgtggca ttttcagtaa agaagtcctg
      301 agtaaacacg ttgacgttga ataccgcttc tctgccgagc cttatattgg tgcctcatgc
      361 agtaatgtgt cagttttatc tatgttatgc ctgcgggcga agaaaacaat ctaaggaatt
      421 tttcaaatgg caaagattaa aggtcaggtt aagtggttca acgagtctaa aggttttggc
      481 ttcattactc cggctgatgg cagcaaagat gtgttcgtac acttctccgc tatccagggt
      541 aatggtttca aaactctggc tgaaggtcag aacgttgagt tcgaaattca ggacggtcag
      601 aaaggtccgg cagctgttaa cgtaacagct atctgatcga atccactgat ctgaagtgtg
      661 aatacgcttc aatctcgcta taaagcctcg tcgaatgcga ggctttttac tttgttttat
      721 cttcgctcca gtcgttcgga tatttgcccg ccgcatgatt cgcgttacac ttgcggcctt
      781 tagtatcctg ccggagttgt catgtcgttt tcctgtccac tttgccatca gcctcttttg
      841 cgtgaaaaaa acagctatat ctgtccccag cgacatcagt ttgatatggc gaaagaaggg
      901 tatgtcaatc tgctgcccgt tcagcataaa cggtctcgtg atccgggcga cagcgcggaa
      961 atgatgcaag cacgccgcgc attcttagat gccggacatt atcagccgct gcgtgatgca
     1021 attgtcgccc aactgcggga atggcttgat gaaaaggcca cggcggtgct ggatattggc
     1081 tgtggtgaag ggtattacac acacgcattt gctgatgcgt tgcccgaaat caccacgttt
     1141 ggcctggatg tctcgaaggt agcgataaaa gcggcggcga aacgctatcc gcaggtcact
     1201 ttttgtgtcg cttccagcca ccgtttgccg ttttccgata ccagtatgga cgccataata
     1261 cgtatttacg cgccgtgtaa agcagaagaa ttagtgcgag tagtgaagcc cggcggttgg
     1321 gtcattactg ccacgccggg accgagacac ttaatggagc tgaaggggct gatttacaat
     1381 gaagtacatc ttcatgcacc tcatgcagaa caactgaaag gttttacatt acagcagagt
     1441 gatgagttgt gttatctgat gcgtcttcgc ggtgatgaag ccgtcgcatt attgcagatg
     1501 acgccgtttg cctggcgtgc gaagcctgaa gtctggcaag cactggcagc aaaagaagtg
     1561 ttcgactgcc agacggactt taatattcac ctctggcagc gttcttatta accgtggaag
     1621 tgcgtccaga ggatctggac gccaataccg atcagtacca gcccaccgag aatttccgct
     1681 tttttcccaa taattgagcc gataaagcga ccaaccatca tccctaatgt cgacataatc
     1741 aaggttgcac aaccaatggc caatgcggtc gcgataatgt taacctgcag gaaagcaaga
     1801 ccaacaccca cagccatggc atccaggctg gtggcaatcg cggtggttac cagtagccag
     1861 aaaccgtgtc gacggcgcgg ttcttcatct tcatcatctg cgccacgaaa accctcaata
     1921 atcattcgcc cgccgagaaa tatcagcagc acaaacgcaa tccagtggtt ccattcaagg
     1981 acaaaccgac tggctaacat gcccattccc cagccgatca gcggcgtcag ggtttcgacg
     2041 gcaccaaaaa taaggccggt tcgcaatgct tcagaaaatt tcggtttatg gagggtggca
     2101 cctttaccga ttgatgcagc aaaggcatcc atcgacatac caaacgcaag aagaacagta
     2161 gcagtgatat tcatgacaac gtcctgaccg gggtatccat aaaacacatc atcgccccca
     2221 gcaaacatgc atacatgaac aacaagccgc gatggttttc gcgttcatgc aggcatagcg
     2281 attgatgtgc atatggtctc gcctgactga aagtgttcag tccgtacgtg ccacgttttg
     2341 caacgagtat gttgacacgt acatttccgg aatctggaaa tcagctactc cccaaatgag
     2401 ggcgcaacct taacat
//
LOCUS       DACADF010000299         2373 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.304, whole genome shotgun sequence.
ACCESSION   DACADF010000299 DACADF010000000
VERSION     DACADF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.304"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..2333)
                     /locus_tag="ISB30_004830"
     CDS             complement(<1..2333)
                     /locus_tag="ISB30_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAP1992357.1"
                     /translation="MQLSDGDNAKNIDLNYFANASGAAPGEYTVDVVMNGELVDRQIK
                     IDFFAQDNKLNARLTPQQLERWGIDTNKLSVSSPSAYQQNIAYIIQGATEKFDANNQK
                     LILNIPQSYLKPQDWLSTPPHLWDEGMPALMVNYLFNGIKQNNNGYGSRSQFLSLDSS
                     LNLGGWRLRHNGNWSTNSYNKESHWQPVSVYLQHDYSFLQGGQFTVGQTSTDGAIFDS
                     FPFEGAQFSSDDGMIAPELSQYSPVVRGIAYSQAQVSVKQNGVVIYQKNVPPGPFELR
                     DFNQIFTGDLEVEIREADGTIRHFTQATAVLPILQRQGRLRYNLAFGKYRSSSTLNNS
                     VSEPQFVQSSAAIGLPYEYTFYGGGIKADNYAAVLLGLGKYSDFFGAFSLDVTHARSQ
                     FSQNYKSFGKQQGQSYRFMYSRGFGETNTTLNITGYRYATRGYYDFDELQQIQSGFVD
                     EKNINSYHQRSRISTTISQDLQDWGQLYLSASKDQYWDTADGYNLSASYSLPFRYISA
                     MLSLGYNKSPYYHEADKSLFLSVSVPLNSLLNGNNLFLTTNTITNNGQVQQQVGLNGS
                     SQDGEFNYAVAQGWQNQNRGESGNINLNYRGPYAQMNGGYAWQKESSQWTYGISGGIT
                     LHPHGLTLSQPLSLDSASALVQARDAASVKVLNGSGIYTDWRGYAVVPYLNPYNRNQI
                     SLDVNSVKDNVELLNTDVTVIPSRGALVSAPFKVNVGNKAIITLIQKNGEPVPFGSVV
                     TLDSENSINSSIVADQGQVYMSGLPEEDTLIAQWGEEA"
BASE COUNT          668 a          534 c          494 g          677 t
ORIGIN      
        1 gcctcctcac cccattgtgc aataagtgta tcctcttcag gtaaccctga catatacacc
       61 tgtccttgat cagcaacaat actgctgtta atgctatttt cagaatctaa tgtcaccaca
      121 ctaccaaacg gtacgggctc accattcttc tgtatcaagg taattattgc cttattaccg
      181 acattgactt taaatggagc ggaaactaaa gctcctcgag aagggatgac agtaacatct
      241 gtatttagca gctcaacatt atccttaaca ctattaacat caagtgaaat ttgatttcgg
      301 ttatagggat tcaaatatgg gaccacggca tatccacgcc agtccgtata aatacctgac
      361 ccgttcaata cttttacgct ggcggcatca cgagcctgaa ccagagcact ggcactgtcc
      421 agacttaatg gctgggataa ggttaatcca tgtggatgca acgtaatacc accgctaatt
      481 ccgtatgtcc attgactgga ttctttttgc caggcatagc cgccgttcat ttgtgcataa
      541 ggtccacgat agttaaggtt aatgttacct gactctcctc tgttttgatt ctgccagcct
      601 tgcgccacgg catagttaaa ctcaccatcc tgtgaggagc cgtttaatcc aacctgctgt
      661 tgcacctgac cattattcgt aatggtattc gtggttaaga acaggttatt tccattcagc
      721 aatgagttga gcggtacaga cacagataaa aacagcgatt tatcagcctc gtggtaatac
      781 gggcttttat tatacccgag cgatagcatt gccgagatat agcggaatgg cagcgagtaa
      841 ctcgcagata agttatatcc atcagcggta tcccagtact gatctttact tgctgagagg
      901 tagagttgtc cccaatcctg aagatcttga cttatcgttg ttgatatacg cgaacgttga
      961 tgataactgt ttatattttt ctcatcaacg aatccactct gaatttgttg taactcatca
     1021 aaatcgtagt aaccgcgtgt ggcataacga tagccggtaa tattcaatgt cgtattcgtc
     1081 tcaccaaaac cacgcgaata cataaagcgg taagactgtc cttgctgttt tccgaaagac
     1141 ttataattct gcgaaaattg tgagcgagca tgagtgacat ccagggagaa cgctccaaaa
     1201 aaatctgagt atttacccag gccaagcaga actgctgcat agttgtctgc tttgatacca
     1261 ccaccataaa atgtatattc ataaggtaat ccgattgctg ctgaagactg aacaaactga
     1321 ggctcagaca cactattatt caaagtagaa cttgagcgat atttcccgaa tgccaggtta
     1381 tagcgtaagc gcccctgacg ttgcagaatc ggtaaaacgg ccgtcgcctg agtaaaatgg
     1441 cgaatagtgc catctgcctc acggatttcc acctccaggt caccggtgaa tatttggtta
     1501 aaatcgcgta attcaaacgg tccaggtggg acgttctttt gataaatgac aactccgttt
     1561 tgcttgacgg atacctgtgc ttgagaataa gcaatgccac ggacaacagg actatattgg
     1621 ctgagttctg gggcaatcat gccatcatca gatgaaaact gtgctccttc aaaaggaaaa
     1681 ctgtcaaata tcgcaccatc cgttgaggtt tgccctacag taaattgccc tccctgcagg
     1741 aaagaataat catgctgtag ataaacacta acgggttgcc agtggctctc tttattatat
     1801 gagtttgttg accagttacc gttatggcga aggcgccatc caccaaggtt cagagaactg
     1861 tccagagata aaaactgact acggctaccg tagccattat tattttgctt aattccgtta
     1921 aatagatagt taaccattag cgctggcata ccttcatccc agagatgggg cggagtactt
     1981 aaccaatcct gaggtttgag gtagctttgt ggtatattca atattagctt ttggttgttc
     2041 gcatcgaatt tctctgttgc gccctgaata atatatgcaa tattttgctg gtaagccgat
     2101 ggggaagata cggagagctt atttgtatca attccccaac gttccaactg ttgaggcgtc
     2161 agtctggcgt tcagcttgtt gtcctgcgcg aaaaaatcta tcttaatctg acggtcaact
     2221 aattccccat tcataactac atcaacagta tattcccctg gagctgcacc gcttgcgttt
     2281 gcaaaataat tgagatcgat atttttggca ttatcaccat cagataattg caaaaaatcg
     2341 gggttaaatg agaacctatg actattgtca ggt
//
LOCUS       DACADF010000300         2364 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.305, whole genome shotgun sequence.
ACCESSION   DACADF010000300 DACADF010000000
VERSION     DACADF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.305"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(497..673)
                     /locus_tag="ISB30_004831"
                     /pseudo
     CDS             complement(497..673)
                     /locus_tag="ISB30_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(936..1064)
                     /locus_tag="ISB30_004832"
                     /pseudo
     CDS             complement(936..1064)
                     /locus_tag="ISB30_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(1037..1195)
                     /locus_tag="ISB30_004833"
     CDS             complement(1037..1195)
                     /locus_tag="ISB30_004833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992358.1"
                     /translation="MMMSDTTLSRPEVVSGHTDVIYSTSVCHILAVRKSTLLPIDTII
                     RHQVSDIS"
     gene            complement(1295..1471)
                     /locus_tag="ISB30_004834"
     CDS             complement(1295..1471)
                     /locus_tag="ISB30_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAP1992359.1"
                     /translation="MKSLTTETALDILITWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IAQAREGIRTLRQQ"
     gene            complement(1488..1976)
                     /locus_tag="ISB30_004835"
     CDS             complement(1488..1976)
                     /locus_tag="ISB30_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992360.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDIDGVNLAELINMVCDNGYS
                     LRVVDESDRTSADCTPPFVALTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GTGYLLRTDTWSYPVLRLKRLGLSKTFRRLVVTLIRCYGVSLIHLDASAGYLPGLPTF
                     DW"
     gene            complement(1973..2350)
                     /locus_tag="ISB30_004836"
     CDS             complement(1973..2350)
                     /locus_tag="ISB30_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP1992361.1"
                     /translation="MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGFSACTHSQLINSIDILRARRATG
                     LMIRDNYRTVNNITLGKHPEAKR"
BASE COUNT          590 a          584 c          571 g          619 t
ORIGIN      
        1 ctgcaacata atctacgctc atccacatgc ctggtgcgta ttaccgtgaa ggagatcggt
       61 gagttattga gtgatctcct ttcatgaaaa tacgcacctg gctttaactg ttcctgatat
      121 aagttgacag ttttctgtcc gtaaaacggt tgatgaactt catcgggcgt tttgtatttc
      181 aatgccaaat gtgaccgctc atggttataa attgccacga attcttttac catttaccgg
      241 ccctacgtca gctctgcagg acgcgagagt aaaaactcat tttcaggatt ccgtttatcc
      301 actcagcctg cgcattctgg tggcagttat aaccattagt cattgaacag gttattgata
      361 aataattaaa aaactcagtt ttctggctcg cctgctcaag ctgttcttca agttccctga
      421 ttctttgctc gggtgtcagc gggacagtcg ccagagccac aggcagtctc ctctttacct
      481 ggtcaggaag tccggggctc cagtcaagcc ggccatattt acgcaaccag acaagaacgg
      541 tggagcgccc ctgaatgcca taactgcagc cggcctgttt ataagtcatc tcgccttttt
      601 caaccttctc aacaacggct attttaaagg atagaggata atcacgttga gtgcgtttga
      661 acccagacat catcacgttc tccgggttta agtcaggaac gtgtcatagc tattcaggac
      721 gggtcaccga cacaaaaaaa gtcgcccttg agcgactcga tttgcatacg gtatggtgcg
      781 aaggccggac tcaaacatgc gatttaacct catgatttaa aattgttaat aataaacaac
      841 accatcctat accaacacag ataccaacac gaaaaatcac tggttttcgg catcgaacca
      901 ggacaatcag gtcctgattg agataccgag ctctgttact cgtcatcttc ctcgacaggc
      961 tccattgcat caagtggtac tcgcaccatc gcgatagaac caatccggat aaaagcatct
     1021 tccccatcta tatcggttaa ctgatatcgc ttacttgatg ccggataatt gtgtcgattg
     1081 gcaacagtgt actctttcgt acagccagaa tgtggcagac tgaggtggaa tagataacgt
     1141 ccgtatgccc gctgaccacc tccgggcggg agagtgtggt atctgacatc atcatttttc
     1201 ctttctgttt ataaatgaaa atgccagccg tgttcaggct gacgtcaggg aagtgaaatc
     1261 gggtgagtga tcttcactgg ttctggtaca aaagttactg ttggcgcagg gtacggatac
     1321 cctccctggc ctgtgcgata cagggcaaca gtgctgccga atctgtttta tcctcatcgt
     1381 tgtcgaagat aattcccgat tcgcagtcga tattgtcctg cagccacgta atcagaatat
     1441 ccagcgctgt ttccgtggtt aatgatttca tgttgtgaat ttccggatta ccagtcgaaa
     1501 gtgggtaaac ccggcagata tccggcactg gcatccagat gaatgagact gacaccataa
     1561 caccggatga gtgtgacgac cagacgacgg aacgtttttg acagccccag gcgcttaagc
     1621 cgcagaaccg ggtatgacca cgtatcggta cgtaacagat aaccggtacc ggtaaaatga
     1681 atccattctg attcaccgaa gtcactggtc tggtgtgaca gcgagtacag ccaggcgttg
     1741 tccgtttccg tgatatgtgc ggtactgcag cgtatgccgg taagggcaac aaatggtggc
     1801 gtgcagtctg ctgaggtccg gtcagattca tcaacaacac gaagggagta gccgttatcg
     1861 cagaccatgt taatcagttc agcgagatta acaccatcaa tgtcaacgac aatgcgcccc
     1921 atgttcagcg cctgcacgtt aacgctgtcg gcttccagcg tcagggccag tttcatcgtt
     1981 tcgcctccgg atgcttcccc agggtaatgt tatttaccgt tctgtaattg tcgcggatca
     2041 tcaggcctgt tgcccggcga gcccggagga tatcgatgct gtttattaac tgagagtggg
     2101 tacaggcgct gaatcccggc aggtcggtac gtaccagtgc gtatttttcc acgagaaagt
     2161 tcactgcatc acacagtgaa atgcctgcct caatatgctg ctcaatcaca cgttcatcag
     2221 cgaacggtgt gtcattcagc gtaaggccgt agtgctggtc cagcagtcgg gtgagcagtg
     2281 tctgccagat ggtgactgga gacgggcagc acgatacctc ccgtacatgc gtgtcgggta
     2341 atgttttcat actgaagatt ttcc
//
LOCUS       DACADF010000301         2346 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.306, whole genome shotgun sequence.
ACCESSION   DACADF010000301 DACADF010000000
VERSION     DACADF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.306"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            11..2095
                     /locus_tag="ISB30_004837"
     CDS             11..2095
                     /locus_tag="ISB30_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAP1992362.1"
                     /translation="MVSTATATSSETKISNEETLVVTTNRSASNLWESPATIQVIDQQ
                     TLQNSTNASIADNLQDIPGVEITDNSLAGRKQIRIRGEASSRVLILIDGQEVTYQRAG
                     DNYGVGLLIDESALERVEVVKGPYSVLYGSQAIGGIVNFITKKGGDKLASGVVKAVYN
                     SATAGWEESIAVQGSIGGFDYRINGSYSDQGNRDTPDGRLPNTNYRNNSQGVWLGYNS
                     GNHRFGLSLDRYRLATQTYYEDPDGSYEAFSVKIPKLEREKVGVFYDTDVDGDYLKKI
                     HFDAYEQTIQRQFANEVKTTQPVPSPMIQALTVHNKTDTHDKQYTQAVTLQSHFSLPA
                     NNELVTGAQYKQDRVSQRSGGMTSSKSLTGFINKETRTRSYYESEQSTVSLFAQNDWR
                     FADHWTWTMGVRQYWLSSKLTRGDGVSYTAGIISDTSLARESASDHEMVTSTSLRYSG
                     FDNLELRAAFAQGYVFPTLSQLFMQTSAGGSVTYGNPDLKAEHSNNFELGARYNGNQW
                     LIDSAVYYSEAKDYIASLICDGSIVCNGNTNSSRSSYYYYDNIDRAKTWGLEISAEYN
                     GWVFSPYISGNLIRRQYETSTLKTTNTGEPAINGRIGLKHTLVMGQANIISDVFIRAA
                     SSAKDDSNGTETNVPGWATLNFAVNTEFGNEDQYRINLALNNLTDKRYRTAHETIPAA
                     GFNAAIGFVWNF"
BASE COUNT          681 a          519 c          536 g          610 t
ORIGIN      
        1 catttctggg atagtttcta ctgcaaccgc aacttcttca gaaacaaaaa tcagcaacga
       61 agagacgctc gtcgtgacca cgaatcgttc ggcaagcaac ctttgggaaa gcccggcgac
      121 tatacaggtt attgaccaac aaacattgca gaactccacc aatgcctcca tagccgataa
      181 tttgcaggac atccccggag tagagataac agacaactcc ttggcaggcc gtaaacaaat
      241 ccgcattcgt ggcgaagcat cctcccgtgt tttaattctc attgatggtc aggaggtaac
      301 ttatcagcgc gccggagata attatggtgt gggactgttg atagatgagt ctgcgctgga
      361 gcgtgttgag gtagtgaaag gtccatattc cgtactgtac ggttcacagg caattggcgg
      421 tattgttaac ttcataacca aaaagggagg tgacaaactt gcatctggag ttgtgaaagc
      481 tgtttataat tccgcaacag caggctggga agaatcaatc gcggtccagg ggagcatcgg
      541 tggatttgat tatcgcatca acggtagtta ttctgatcag ggcaatcgtg atacgccgga
      601 tggacgtctg ccgaatacca actatcgtaa caatagtcag ggtgtatggt tgggttataa
      661 ctccggaaac catcgttttg gcctctcgct tgatcgctac agactcgcga cgcaaactta
      721 ctatgaggat ccagacggaa gctatgaggc atttagtgtc aaaataccta aacttgaacg
      781 agagaaagtt ggggtattct atgacacaga cgtggacggt gactatctaa aaaaaattca
      841 tttcgacgcg tatgagcaga ccatccagcg ccaatttgcc aacgaagtaa aaacgacaca
      901 gcctgttccc agtccgatga ttcaggctct gaccgttcat aacaagactg acacccatga
      961 taagcaatac actcaggcgg tcacattgca gagtcacttt tcgctgcctg ctaataatga
     1021 acttgttacc ggtgcacagt acaaacaaga cagggtcagc caaaggtccg gtggcatgac
     1081 ctcaagcaaa tctctgaccg gcttcattaa taaggaaaca cgaactcgct cctattatga
     1141 gtcagagcaa agtacagtct cactattcgc acaaaatgac tggcgattcg ccgatcactg
     1201 gacatggaca atgggagttc gccaatactg gctttcttca aagttgacgc gtggtgacgg
     1261 agtatcatat accgcaggca ttataagcga tacctctctt gccagagagt ctgcgagtga
     1321 tcacgaaatg gtaacatcta caagcctgcg ctattcaggt ttcgataact tggagttacg
     1381 cgctgcgttc gcgcaaggct acgtatttcc cacactctcc cagcttttta tgcagacatc
     1441 tgcgggcggc agtgtcacat acggaaatcc tgatcttaag gctgaacact ccaataactt
     1501 tgaattaggt gcacgatata atggtaatca gtggctgatt gacagcgcag tttactactc
     1561 agaagctaaa gattatattg caagtctgat ctgtgatggc agtatagttt gcaatggtaa
     1621 caccaactcc tcccgtagta gctactatta ttatgacaat attgatcggg caaaaacatg
     1681 gggactggaa ataagcgcgg aatataatgg ctgggttttc tcgccatata tcagtggcaa
     1741 tttaattcgt cggcaatatg aaacttcaac attaaaaaca actaatacag gagaaccagc
     1801 gataaacgga cgtatagggc tgaaacatac tcttgtgatg ggtcaggcca acataatctc
     1861 tgatgttttt attcgtgctg cctctagtgc aaaagatgac agtaacggta ccgaaacaaa
     1921 tgttccgggc tgggccactc tcaactttgc agtaaataca gaattcggta acgaggatca
     1981 gtaccggatt aacctagcac tcaataacct gacagacaaa cgctaccgta cagcacatga
     2041 aactattcct gcagcaggtt ttaatgcagc tataggtttt gtatggaatt tctgatgaca
     2101 aaaacaatcg ttatggaatt ctttgtcctg atctgcccgc tttgcgggca ggctaacgaa
     2161 cgtattgtgt ttgttgaagg tttttcgacg ttgttttcca tctgggggtg aatatttatt
     2221 cttcccgcca gcatgactct atagtcattg ctacgacaat gacttataaa gctggttact
     2281 gagcctatac ctttgtaatt caaaagtgat atcagttcac aacgcaatac tatttttaga
     2341 ttattt
//
LOCUS       DACADF010000302         2342 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.307, whole genome shotgun sequence.
ACCESSION   DACADF010000302 DACADF010000000
VERSION     DACADF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.307"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            107..610
                     /locus_tag="ISB30_004838"
     CDS             107..610
                     /locus_tag="ISB30_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP1992363.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(656..1072)
                     /gene="rraB"
                     /locus_tag="ISB30_004839"
     CDS             complement(656..1072)
                     /gene="rraB"
                     /locus_tag="ISB30_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAP1992364.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1234..2238
                     /gene="argF"
                     /locus_tag="ISB30_004840"
     CDS             1234..2238
                     /gene="argF"
                     /locus_tag="ISB30_004840"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP1992365.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNTLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNNKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          601 a          548 c          583 g          610 t
ORIGIN      
        1 gtaataggct ggcaaaaact ttattgcttt ataagacgct tgcgcacgta agaagagagt
       61 ggtattaaac aaatgtataa ttactttatt tttattgggt taaactatga ataacattgc
      121 gccgcaatca cctgtaatgc gtcgcctgac gttgcaggat aatcctgcta tcgcccgcgt
      181 cattcgtcag gtatccgccg aatacggtct taccgctgat aaaggctaca ccgtcgccga
      241 tccgaatctt gacgagctgt atcaagtgta tagtcagcct ggtcatgcat attgggtggt
      301 tgagtacgag ggtgaagtgg tcggcggcgg tgggattgcg ccattagcag gtagtgaatc
      361 ggacatttgc gaactgcaaa agatgtattt tcttccggct attcgcggta aagggctggc
      421 gaaaaaactg gccttaatgg cgatggagca agcacgagag atgggcttca aacgctgcta
      481 tctggaaacg accgcttttt taaaggaagc cattgcgctt tatgagcatt tgggctttga
      541 gcatatcgac tatgcgcttg gctgcacggg ccatgtcgat tgtgaagtgc ggatgctacg
      601 cgaactctaa aaaaatgccc gctagtgtgt gctatcgggc attttaatca attaattagt
      661 ggcgaacacc gtcatcgtct tcatcgacaa aatcttcatc gtcgccgtct tcgccattgg
      721 gatcttcaaa gtaagtgccc caaccgtcgt actcaacgtc aaatttctcc gccagcgtca
      781 tcagttgttc aacctgggca tcgatcagat cggcattcaa cgcgcactcg ctgaggatgt
      841 cgcagcaaat cacgatatca ccatcttcaa cttccagctc ttctggatcg gtcacttcgt
      901 aaccgagttt aaacgcttca actgctgctt tttccagggt ttccagatcg tctgcggaaa
      961 gatggtgttc gatggtgtac agcgcgtccg gatcgctgcc atcttccagt aattcttcaa
     1021 taatcaaacg tgtttcttca cgctgttctt ccagttgttc cgggtttgcc atggctcatt
     1081 cctcttaatg tcctgccgat actcttattg tcacacacca ctgacattgc ctccaccttt
     1141 gtgacaaaga tttatgcttt agacttgcaa atgaataatc atccatataa attgaatttt
     1201 aattcattga ggcattagcc acaggaggga tctatgtccg ggttttatca taagcatttc
     1261 ctgaaattac tcgatttcac gccagctgaa ctcaacacct tgttgcagtt agccgcgaag
     1321 ctgaaagccg ataagaaaag cggtaaagaa gaagctaaac tcactggtaa aaacatcgcg
     1381 ctcatcttcg aaaaagactc gactcgtacc cgatgctctt tcgaagttgc cgcatatgac
     1441 cagggtgctc gtgttactta tctcggccca agcggcagcc agattggtca taaagagtcg
     1501 attaaagaca ctgcccgcgt gcttggtcgc atgtatgacg gtattcagta tcgcggctat
     1561 ggtcaggaga ttgtcgaaac actggcggaa tacgctggcg tgccggtatg gaacggcctg
     1621 accaatgagt ttcaccccac gcagctgctg gcggatctcc tcaccatgca ggagcatttg
     1681 cccggtaaaa cattcaacga aatgacgctg gtctatgcag gtgacgcacg taacaacatg
     1741 ggcaattcga tgctcgaagc tgcggcgctt accggtctgg atttgcgtct ggtcgcgcca
     1801 caggcatgct ggccggaagc tgcgctggtt acggaatgcc gcgctctggc acagcaaaat
     1861 ggcgggaata ttacgctgac ggaagatgta gccaaaggag tagaaggtgc tgactttatc
     1921 tataccgatg tgtgggtgtc gatgggggaa gcaaaagaga aatgggcgga acgaattgca
     1981 ttgctgcgtg attatcaggt gaataacaag atgatgcagt tgaccggtaa cccggaggtc
     2041 aaattcctcc actgcctgcc cgcatttcat gacgaccaaa cgacgcttgg caagaaaatg
     2101 gcggaagagt ttggcctaca tggcggaatg gaagtgactg atgaggtctt cgaatctgcc
     2161 gccagcattg tatttgatca ggcggaaaac cgcatgcata ccatcaaagc ggtgatggtc
     2221 gcgacgctca gtaaataatt gatatcgcta taaagatgcc gggaaataca aaactatccc
     2281 ggcatcacat tattttatgc agtgataggt ctattcttcc acccaatttt ttctattgca
     2341 ga
//
LOCUS       DACADF010000303         2313 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.308, whole genome shotgun sequence.
ACCESSION   DACADF010000303 DACADF010000000
VERSION     DACADF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.308"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            722..1269
                     /locus_tag="ISB30_004841"
                     /pseudo
     CDS             722..1269
                     /locus_tag="ISB30_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
     gene            1601..>2313
                     /locus_tag="ISB30_004842"
     CDS             1601..>2313
                     /locus_tag="ISB30_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein"
                     /protein_id="HAP1992366.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATAQHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQILALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAG"
BASE COUNT          610 a          526 c          568 g          609 t
ORIGIN      
        1 ctatagatgc agggaaagct aagaataagc ttatagctgg ggctgttttt ttgttgaatt
       61 tccagtttgt gactctgatt attacagcta tagtcatcca gactataatt aaaatagaac
      121 tgccattgat aaaatcatat ttcaaaataa attctcctgt atttgtcact caatcggtgg
      181 tttatgtgac tatattcgca aaaactggtc gcagaaaagt cctgttttca gcttggaaac
      241 ccggtaatca gaataatgga taggggaaca aaaatctctg tatagaacgc tccattgagg
      301 gagagaaaac gtgtcatttt gacccgttcg gtattcagaa cacacaaaac cgatcgcggg
      361 tcgctttcgt gcatagttat gcagccatct aaaaacgatt ctggcgcgtt tttctggttt
      421 agcctggtgt tttcttgtct ttttgcgttt tttgcgccag aaagcgattg agggcgtttt
      481 acagggtacg tacaacggga gttatggtaa atggatcggg ttttcgggaa ggttcgacag
      541 gatttgccgt tgggtgtagt gtaagcgact gaaaaacaaa cgcccctgaa atcatgtcca
      601 gatccggcag aaccagcctc atgcgcagtt ctggctggtt tttgacgttg atcgcgaggg
      661 ggcagcgatt gactggagcg accggaacgc tcccgcgccg aacatcaccg taaaaaatcc
      721 tgtgaacggt catgctcacc tgctctacgc gctcaatatc gccgtgagaa ccgcgccgga
      781 cagctcggtt aaggcgctga aatacgccgc tgcgattgaa cgttcgctgt gtgagaaact
      841 ttgtgctgat gtgaactaca gcggactgat ttgtaaaaat ccgttccacc tggaatggca
      901 ggtgatggag tggcgagagg aagcctatac cctcgatgaa ctggctgatt atctcgattt
      961 gagcgcctca gcgcgccgta gcatcgacga aactgccacc tgttcgaaat gacgcgcaaa
     1021 tgggcttaca gggcgattcg tcagggctgg cccgaattct cacagtggct tgatgccgtg
     1081 attcagcgtg tcgaaatgta caacgcatcg cttcccgttc cgctttcacc gcctgaatgt
     1141 cgggctattg gcaagagcat tgcgaaatac acgcacagga acttcacgcc ggaaactttc
     1201 gcacagtatg tggctgatac gcacacatta acgtacgttt tcgttccatt ggccctcacc
     1261 ccccgataac gacccaggta gcgcgccgta ttccccccgc cggccgtttt cttcgacatg
     1321 tacatcaggc gcggaactca tcggggcgca ggaatgaaat cccattcaga ctccggatag
     1381 attcaagcaa gcccacttat cgtcaaaatc ggtgttgcaa aaacgggagt gaccatagat
     1441 ccgttttcca agcggaccca taatatcggc tggtaaacat cactcgctag tgcattcttt
     1501 ttgttggtaa tgataatcat ttacattcat tctttgatgt tttatcataa ttaatgtttt
     1561 gtcaataaat atatggaaac atattgtttc agatgggaat atgagaaagt tcatccttat
     1621 ttctatgata acgttagcta gtgttagtct gggagcctgc agacagaatg tcaccataaa
     1681 gcaggatgcg ccagggcaga aagctttcct gactgacggg cagatttacg atttgcacag
     1741 tggcaaaatt atttctagca gcgagctttt ggcggatctt gctaccgctc aacatcttat
     1801 tattggtgaa aaacacgata atgccgagca tcatcagatt gagctttggc ttattcaaaa
     1861 tttgctcatc cagcgaccac aggggagtgt gttgcttgag atgttgacgt cagagcaaca
     1921 gccccgagtt aaccaagtta aatgttggct gaaagataat cctgtggttc gagatagccg
     1981 tgttcaggaa ctacttaact ggcaaaaagg gtggtcctgg gagatgtatg gcgacatcgt
     2041 catgcagcta ttacgtgggc cttacccgct gctcaatgcc aacattggcc gggagcagat
     2101 attggcactg tacaaaaaaa acgaatttcc taaaggaaaa aaatccactg cacctgtggt
     2161 acaagaagct ctgcgtgaaa ccatcatatc tatgcatgaa ggcaaccttg aaagccagca
     2221 actgacgtca atgctctcta ttcagcagca gcgggatcgc tacatggccc gccagcttct
     2281 gagtgccccg gtaccgtcgt tgttgattgc agg
//
LOCUS       DACADF010000304         2284 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.309, whole genome shotgun sequence.
ACCESSION   DACADF010000304 DACADF010000000
VERSION     DACADF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.309"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..483)
                     /locus_tag="ISB30_004843"
     CDS             complement(<1..483)
                     /locus_tag="ISB30_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP1992367.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     N"
     gene            complement(535..2217)
                     /locus_tag="ISB30_004844"
     CDS             complement(535..2217)
                     /locus_tag="ISB30_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP1992368.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
BASE COUNT          606 a          470 c          507 g          701 t
ORIGIN      
        1 attaataacg gttaacgtat taaactctat ttgatatgat ttcagacgct cgatggctgc
       61 tatcactttt gcaaaagtac cgttacctga attactgcgt ctgtaacggt catgtagttc
      121 ttgggggcca tcgatcgaga taccaaccag aaactcatgt tctttgagaa aggcacacca
      181 ttcattattc aataaaatgc cattcgtttg taatgcatta aaaatacgtt tttggcctgc
      241 atagcgttgt tgatagtgaa taactttacg gaaaaaatcc aggccagcca gagtgggttc
      301 accgccttgc caggtaaaat agacctgatt gccagacgct gcgatatatt gtttgatgaa
      361 ctctttcaga gtgctgtcat ccatccattt ttcatgagta aactgcgact ctttttcaag
      421 gtaaaaacag taatcacatt tgagattaca ttgaaaactg gagggcttgg ctgtaacgtg
      481 catcgctatc tcgctcaata aggcggcgga aaaatccgcc gcataaaggt ttagttattt
      541 cgcttcgctt agtgctttct tgatattgtt aaacttctcc tgatttacct cgctaagcgg
      601 tggctggctg ttatcgataa actctcttac cacgccttgc atctctttaa cgacctgcgg
      661 attggcggcg gcaaggttat ctttttgctg tagatccgtc agtttgtata gacctaactg
      721 attattttct actgtataga caagcgaata atcgttattt ctcaccgtat aagagaattg
      781 gcttaagtcc tcggtgttgg ggttatgcgg gtaatcgtct gactgatggc ggacaaattt
      841 gtggtaatta tcccagaatg gaatattttc ctcatcaaac cagtgagaat aagatgttat
      901 ccaggtcaga tttttatgtg gctcgccttg tttcttattt tgcaaccagg gcagcaagga
      961 aacgccatcc agcttaaggt cttttggaat gctgatatcg gctgcatcaa gagctgtcgg
     1021 gtagaaatcc attgcggaaa tcagcttgtc ataattaccg ggttgaagtt ttcctttcca
     1081 ccacataaac attggggtgt gagtaccgcc aggataggtc tgactcttat agcctttttg
     1141 cgccccgttc agcggcagag gaccatcgat aactgcacca ttatcggagg taaagagaat
     1201 aattgtattg tcatactgtc cgtttttctt cagttgttcg agaatgcgtt ttacaccctg
     1261 atcaacagaa taaacggaag cgtagtaatt atctgctgtt tgactaccgg tattaaattg
     1321 cttctgatat tgctccggag caggattatc atttggcagg tgcggagcat tataagccag
     1381 gtaaagcata aaaggctggt caagtgtttt ggcacgatca acaacgccaa ttgcttcatc
     1441 ggttaactga tcgctgatat aaccttttgc ggggacgcgt tcacggtttt tgaacagtga
     1501 aggggagttg taatatgccg ttcctgcagc atggaatccc ataaagtaat caaagccacg
     1561 gttttgcggt tgccattctt ccgcagaaaa ggtagtgaag ttgtcatgat agtcacgcgt
     1621 ttgtttatct tccggcaccg gcacattact gatttttgat aagtgccatt tacctactgc
     1681 tgcagtgtaa taaccatgat tctggaataa ttcaggcaag aaagtttctg ttagcggaat
     1741 accatcctga gcatcagtat tggaatagac accaaagcgg gcgggcgctc gaccggtcat
     1801 tattgcggcg cgggaggggc cggaaacacc gtgcgccaca tagccgttag taaaacgtac
     1861 gccttcatcc attaatgaaa ggagcgtcgg cgttgatttt tgcgcagctt caatggcttt
     1921 atctatccct attttgtagg tatcgacaac ttcacgattt tccattgttt ttgggtcaaa
     1981 agatccctta tcaaaaggaa gttgtccata accaagatca tccatggtca gtacgataat
     2041 atttggcttt cctttggtac tgtattcagt cggcgtaaag tctgagaacg caacgtttgt
     2101 tttggttgct ttcagcttta catcatctgc cgcatgagca gcaaatgcag ccataccaga
     2161 tgccagtatc aaagatatcg aggtacttac gacacttttc tttaatgcag acttcataaa
     2221 tgttcctctt cttattgtac ttaattccaa agtaatttta cgaagagaaa taagtgaatg
     2281 taag
//
LOCUS       DACADF010000305         2268 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.310, whole genome shotgun sequence.
ACCESSION   DACADF010000305 DACADF010000000
VERSION     DACADF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.310"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(156..1148)
                     /locus_tag="ISB30_004845"
     CDS             complement(156..1148)
                     /locus_tag="ISB30_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1992369.1"
                     /translation="MSIKQLKDGRYQVDVRPQGAEGKRIRKIFALKSKAQEFEKYVLQ
                     NFHDKPWQAKPADQRRLSELLDAWWMLDGRNQAYGDSYRVRLGKVVREMGDPRASQMT
                     RKFMLEYRSEKLQAGLMPSSINRDLCVLSTMFTVLIEAEVFHNANPVRGIRKLKVQNT
                     EMAFLSDDEIERLLERLEGDVRRVAILCLSTGARWSEASELRGEHIVGNRVTFFNTKN
                     GKSRSVPVADSVVPLIKTRRTGLLYQVDYLKFREILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLLDAINFNPLAESVHKLSID"
     gene            complement(1218..1559)
                     /locus_tag="ISB30_004846"
     CDS             complement(1218..1559)
                     /locus_tag="ISB30_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1992370.1"
                     /translation="MVILSIDYAKKLRQIRKAEGLTQKQFADITGLSLATIRNYESGQ
                     KNARAKIVEAVLQVDRFEKYMLWLIKDKTLPVAGQIAPALSLDGSIQSEGDQVSIGIT
                     QKSPRSGRNVG"
     gene            1664..2185
                     /locus_tag="ISB30_004847"
     CDS             1664..2185
                     /locus_tag="ISB30_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1992371.1"
                     /translation="MRDFIADKELSDHQPLQESDALSGSIDAANDDEHEENKKKRSYK
                     KGATLRLDGPIAGLCSLEKGAAYIGLTKSALRVAIHRGQMPGHKTRTNPEDENSDGVW
                     WFNAKEWDKLADELPEHEPPEWHNWKSYWTYDRQKRKFSPANKEDCQTINGKRVYTGR
                     SSKLDQLRNNRGN"
BASE COUNT          667 a          577 c          477 g          547 t
ORIGIN      
        1 tattacttgt aagtgaggag taattattgt tcggtatcaa agggagacaa aaaaagcgcc
       61 cgcaggcgct ttaacgtttc ttgcccctcc tgatgattta cttatttttc aacgaataag
      121 ccaaaacagg gggaacaaat taacacaaat tataactaat cgatggacag tttatggaca
      181 ctttcagcca gcggattaaa gttgatcgcg tccagtaaaa agtccggcga aaaatgagcg
      241 tacgtcattg tttgctgaat cgttgcgtgc cccaggattc gttgcaacgt aacaatattt
      301 ccgccattca tcataaagtg tgtggcaaat gtatgacgca tgacatgcgt agcctgcccc
      361 ttcggcaaat ccggtttcac ctcctgaaga atctcccgga acttcagata atcaacctga
      421 tacaataacc ccgttcgacg ggttttaatc agaggcacga ccgaatccgc cacaggaacc
      481 gaacgggatt ttccgttttt ggtgttaaag aacgtcaccc ggttgccaac gatatgctct
      541 ccacgtagtt ccgatgcttc actccatcgg gcaccagtag aaagacacag aatggcaaca
      601 cgacgcacat caccctccag tcgctcaaga agccgttcaa tctcatcatc agaaagaaag
      661 gccatctctg tgttctgaac tttcagtttt cgtataccgc gtacagggtt cgcgttgtga
      721 aagacctcag cttcaatcag caccgtgaac atggtagaca gtacgcataa gtcacgatta
      781 atactggacg gcattaatcc ggcctgtaat ttctctgacc gatattcaag cataaatttt
      841 cgcgtcatct gactggcgcg aggatctccc atttcacgaa caaccttccc tagcctaacc
      901 ctgtagctat ccccgtaagc ctgattacgc ccatcaagca tccaccacgc atcaagcagc
      961 tctgataatc gccgctgatc agctggctta gcctgccatg gcttgtcatg aaagttctgt
     1021 aacacatact tctcaaactc ctgagccttt gatttcaggg caaagatttt tcgaatccgc
     1081 tttccttccg ccccctgcgg tctgacatcg acctgataac gtccgtcttt gagttgctta
     1141 atcgacatga ttagcccctc caacggataa attaactaaa cactccttgc gatatgtaat
     1201 tcgctcaatg tgtagagtca gccaacattg cggcctgatc ggggtgattt ttgggtaata
     1261 ccgattgaaa cctgatcgcc ctccgactga atagatccat caagagagag agccggagca
     1321 atctgcccgg cgacaggtag cgtcttatct tttattagcc acagcatgta tttttcaaag
     1381 cgatcgactt gcagaaccgc ctcaacgatt tttgctctag cgttcttttg ccccgattcg
     1441 taattcctta tggttgccaa agacaaccca gtaatatccg caaactgctt ttgtgttaac
     1501 ccttccgcct tacgtatctg acgtaacttt tttgcgtagt ctattgacaa gataaccatt
     1561 tgagtattaa cctctcgcac aaagataacc ttttggttat ctttacaagc caaaacaaac
     1621 catcacaagg cgcgacaagc gcaccaacgc aagaggataa caaatgcggg acttcattgc
     1681 cgataaagag ttatctgacc accaaccact tcaggaaagt gacgcactca gcggatccat
     1741 tgatgccgcc aacgacgacg agcacgaaga aaacaagaaa aagcgttcct acaaaaaagg
     1801 agcgactttg cggctggatg gtccgatcgc cgggctttgc tctctggaaa aaggcgcggc
     1861 atatattggg ctaacaaaat ctgcactgcg cgtagccatt catcgcggtc aaatgccggg
     1921 acacaagacg cgcacaaacc cggaagatga aaactcagac ggagtatggt ggtttaacgc
     1981 caaagagtgg gacaagttgg ctgatgagct accagagcac gagcccccag aatggcacaa
     2041 ctggaaaagt tactggacgt atgaccgcca gaaaaggaag ttctctccag ctaataaaga
     2101 agactgccag accattaacg gtaagcgagt ttatacaggc agaagctcaa aactggacca
     2161 actcagaaac aacagaggca actaatcgta tgaaaacaaa cacgtcagat ttcacaataa
     2221 ctatttcaaa aaatgaccaa gcattaattg ataacctgaa ataccttg
//
LOCUS       DACADF010000306         2249 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.311, whole genome shotgun sequence.
ACCESSION   DACADF010000306 DACADF010000000
VERSION     DACADF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.311"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..291)
                     /locus_tag="ISB30_004848"
     CDS             complement(<1..291)
                     /locus_tag="ISB30_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992372.1"
                     /translation="MKRAILLVLIAAGLLAGCGEKTPKCSSDDAKNLVGDIARKTIEK
                     GMKLDKDVRISVENVRTISHESDLDVYQCAADLTFTKPDLQNSLPITYRIQKT"
     gene            complement(322..681)
                     /locus_tag="ISB30_004849"
     CDS             complement(322..681)
                     /locus_tag="ISB30_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005284729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAP1992373.1"
                     /translation="MNRKLLALFMAAITLPVAAAQYVKPGAVLTLHPGQNNAEFNINA
                     STGDGSGVCNMEGTAQQIAAGESQKRRWVWNDNASECVAVISELTNGKASIMTRGCEG
                     YCGASAAGAMDGLFNKK"
     gene            complement(696..1223)
                     /locus_tag="ISB30_004850"
     CDS             complement(696..1223)
                     /locus_tag="ISB30_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992374.1"
                     /translation="MATTITEITECFEYFFSSLYRREFVKTLRLNECSERELLPLVRC
                     YLLGWFADNVSPEVKSKLPGTVSGHGYIDFVIDDVAVEFAVRKPTAARSNVSATVNST
                     EVKKLMKHDGKALLVLFDFSDTPYSEEQIESFRNWPSLGRGNHRKSAFNVVYFFVEKR
                     RPLALGKITKNIRIT"
     gene            complement(1235..1504)
                     /locus_tag="ISB30_004851"
     CDS             complement(1235..1504)
                     /locus_tag="ISB30_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP1992375.1"
                     /translation="MRNSTASQKKQIKGSSGNWHRADIVAALHKRGLTLALLSRSHGL
                     APRTLNNALERHYPRAERIIAGALDTTPETLWPERYALKNGPNSR"
     gene            1758..2114
                     /locus_tag="ISB30_004852"
     CDS             1758..2114
                     /locus_tag="ISB30_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006324788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992376.1"
                     /translation="MRLEKLASLHPEIRRKRTAGKGVVYHISAATMSLDDERNRAKGE
                     AFTLNDSDRQLSLWVQLYRNMSPASRAQLMEQALRLVKDDLMSQGPNVSELAVQIMKL
                     PEDERKRLFEKLNGKV"
BASE COUNT          568 a          539 c          511 g          631 t
ORIGIN      
        1 agttttctga atccggtagg tgattggtag agaattttgc aaatctggtt ttgtaaacgt
       61 taaatcagca gcgcactgat aaacatccag atctgattca tggctaattg ttctgacatt
      121 ttctacagaa atacgcacat ccttatccag tttcatccct ttctcgattg tctttcttgc
      181 aatatccccg accagatttt ttgcatcatc actactacat tttggcgttt tctcgccgca
      241 gccagccagc aaaccagcag caattaatac cagaagtatt gctcttttca tctgtagttc
      301 cttcgtaaat taatgatact tttatttctt attaaataga ccatccattg cgcctgccgc
      361 agaagccccg cagtatccct cgcaaccacg tgtcataatg ctggccttac cgtttgtcag
      421 ttcactaatt actgcaacac actcgctggc attgtcattc cagacccagc gacgcttctg
      481 actttctccg gcagctattt gctgtgctgt cccctccata ttgcatacgc ccgaaccatc
      541 ccccgttgag gcattaatgt tgaattcagc gttattctgt cccggatgca gggttagaac
      601 cgctccgggt tttacgtact gcgctgctgc gactggtagc gttatggctg ccatgaataa
      661 ggccaacagt tttctgttca ttaattaatt ccctgttaag tgatacggat gtttttggtg
      721 attttcccca gtgccagcgg tctgcgtttt tccacaaaga aataaacgac gttgaatgca
      781 gacttgcgat gattccctcg accaagagag ggccagttgc ggaaggattc aatctgttct
      841 tctgaatagg gtgtgtcaga gaaatcaaac agcacaagca aggctttgcc gtcatgtttc
      901 atcaatttct ttacttctgt actgttgacg gtcgcagaga cattggaacg tgccgcagtt
      961 ggtttacgta ctgcaaactc aacggccaca tcgtcaatga caaaatcaat gtaaccgtgc
     1021 ccggatacag ttcctggcag tttgcttttt acttccggtg agacgttatc tgcaaaccaa
     1081 cccagcaaat agcaacgtac cagaggcagt aattcccgct cgctacattc gttcagacgc
     1141 agggttttga cgaactccct gcgataaaga gaactgaaaa aatactcaaa gcattccgtt
     1201 atttcagtga tggtggttgc catggttgca tcctttacct gctattaggt ccatttttaa
     1261 gggcatacct ttccggccat aatgtttccg gtgtcgtatc cagagccccg gcaataatgc
     1321 gctcagctct gggataatga cgctctaagg cgttatttaa tgtccgtggc gcaaggccgt
     1381 gactgcgtga cagtaacgcc agcgttaacc ctcgcttgtg aagtgctgcc acaatatcgg
     1441 cacgatgcca gttccctgaa cttcctttga tttgcttttt ctgcgatgct gttgagttac
     1501 gcacagtatt aactccatgg aatcagtaca tggatattaa atgcgaataa aagatacctt
     1561 tcaagttctt ttggtatcta tttattggtt ttcatattaa cgcattgata tctggtgaaa
     1621 tttatatgag cagtaagaga acagtttcgg ctatacctac tcatcctgaa gcttctgttg
     1681 aagaggctaa agactggctg acagcagagg aattcactca tattgagggg atgccgggca
     1741 ctgcgcgtgg cgccaggatg cgcttggaaa agctggcatc cctgcacccg gaaatacgtc
     1801 gtaaaaggac tgccgggaaa ggtgtggttt accatattag cgcagccaca atgtcgcttg
     1861 atgatgagag aaacagggct aagggcgaag cgtttactct caacgacagt gatcgccagc
     1921 taagcctctg ggtgcagctg tatcgcaata tgtcgccagc ttcgcgggcg caactgatgg
     1981 agcaggccct caggctggta aaggacgatc tgatgtctca ggggcccaat gtttctgaac
     2041 tggcggtaca aatcatgaag ctgcccgaag atgagcgtaa gcgcctcttt gaaaagctga
     2101 acgggaaggt gtgatagcgt cagttttccg ccatgccaga cagaaggaac caccccggaa
     2161 tcctgacctg tctgacatgg cagaatggaa agtatgccat cacttaataa tgaataaaaa
     2221 cctattgcca gttatttcat tgctgaacc
//
LOCUS       DACADF010000307         2203 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.312, whole genome shotgun sequence.
ACCESSION   DACADF010000307 DACADF010000000
VERSION     DACADF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.312"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(359..1831)
                     /locus_tag="ISB30_004853"
                     /pseudo
     CDS             complement(359..1831)
                     /locus_tag="ISB30_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(1983..>2203)
                     /locus_tag="ISB30_004854"
     CDS             complement(1983..>2203)
                     /locus_tag="ISB30_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAP1992377.1"
                     /translation="SGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWGAGVQFNPTES
                     VTIDLAYEGSGSGDWRTDAFIVGIGYRF"
BASE COUNT          383 a          639 c          607 g          574 t
ORIGIN      
        1 caacttttgt cagggttgcc agactggcaa catcattccc cgtaaaatac ggaaacctgt
       61 ctgccgcagt agcaagcccg gccagcgccg tcagggtggc atctttcggt tgcttacccg
      121 caagcgcgct agtcatggtg gtcgcaaaat tcgggtcgtt gcccaacgcc gcagccagct
      181 cgttcagcgt attcagtgcg tcaggcgacg agtccacgag ggcagcgatc gcagccataa
      241 cataagccgt gcttgcgatt tgggtattat tcgttccctg tcgcgcagtt ggtgttgttg
      301 gcgttccggt cagtgcagga ctatttaagg gcgctttctt gttcgtttca cccattaccg
      361 ccttaaccgc ttttggcgtt gccgccagtg actcggaagt gctgttggtc gcactgctga
      421 gctgtactat cccctttttc gtcgtgctcg catcctcaag cgccacggcg gatgcaatat
      481 cctctgcccg ttttgccgct gtctcggcgc gcgttgccgc ggattccgcc gtacttttgc
      541 tctgagctgc cgccgtcgca ctgccagctg cctctgtcgc cttcgtggat gccgtcgtgg
      601 cgctgccctt tgctgctgac gcctgtctgg tcgcctcatc ttttgaagca gacgcagatg
      661 atgccgatga cgccgctgaa ctggctgacg atgcggctgc cgccttagag gaagcagcat
      721 tgtctgctga agtctttgca tttgtttcag aggtttttgc tgcagaagca gacctcgctg
      781 ctgcagtggc ttgctcagtg gctttgccag ccttcgttgt ggctgttgaa gcggatgatg
      841 cggcgctttc tgccgatttt ccggcggcgg tggcactggc tgaggcctgc ccggcacttg
      901 ttgacgcggc actggcagat aatgcagccg ctgtttttga acctgccgca gctgaggcgc
      961 tctgtcccgc tgctgtttca gaagacttag cgttcgtctc ggacgttttt gccgccttcg
     1021 cggaatttcc tgccgccgtt gccgaggaag ctgcactact ggcacttgat gatgcattcg
     1081 tttctgaaga tttcgctgcc tcttttgagg ccgccgcacc ccgtgccgag gtggcagctt
     1141 ctgacgcctt cgtggtcgct gtggatgcag aagtggctgc cgatttttgt gatgctgcgg
     1201 cattcgtttc tgacgttttc gcggcactgg cgctggtagc tgccgcgctt tttgatgact
     1261 ctgcagcagc agcacttttt gctgcttcac gggcctttgt cgatgccgtt cctgcgctgg
     1321 aagacgctga ctgagccgac gacgcggcct gtccggctga cgtgctggcg gcacgtgatg
     1381 aggctgcagc atcggttgca tgagttgccg cctcgctggc tgatgcactg gcatcgctgg
     1441 ctgatttttt cgcggctgcc gtattctgtg caaccgcgga ggcgttacgt gacacctctt
     1501 ctaccatctg ctcaaagcgg cgcagtgcct ccggtcgtac atcatcctcc gtcatggcac
     1561 cgagaaaatc attcagcgta cctggtctgg aaccttcata gacggtaatg gtcccggcat
     1621 gtgaaggcgg aaaaccttca accagcaggg tgacgctgta ctgaccatgc tcaacatcca
     1681 tgctgtaacg tccggcttca tccggatttt cagaggccac cgtgttcacc accaccgtgc
     1741 tgctggttcg tctggccttc agcacaatgg tgcagttctg tactggtttt cctgtgccat
     1801 ctttaagcac gccagaaatt tttactgtca tacttttcca ccaataaaaa aagcccgcag
     1861 cagtgacgcc acgggcttca ggacagtgta actttacgtt tcctcaaacg cagttcaccc
     1921 cataaggtgg atgaacctgc gtatcataac aatatttaca gaagataaat cggcgtctgt
     1981 tgtcagaaac ggtatccgat accaacaata aatgcatccg ttcgccagtc gccactaccg
     2041 gaaccttcat aagcaaggtc aatggtcacg gattcggtcg ggttaaactg cacgccagcc
     2101 ccccacgcca gagacgtgtt gctgtggcga ccgtcatcac ttccggtcag cacatcgtgc
     2161 gttttcccct tgttgtcagt tacgcggaga taatccccgg aga
//
LOCUS       DACADF010000308         2137 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.313, whole genome shotgun sequence.
ACCESSION   DACADF010000308 DACADF010000000
VERSION     DACADF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.313"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            85..642
                     /locus_tag="ISB30_004855"
     CDS             85..642
                     /locus_tag="ISB30_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1992378.1"
                     /translation="MAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWDSG
                     AYNAMIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGFELGWSDFYTT
                     TRKPSAGDVGAYTRTECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYVVTGSVNGNRD
                     ELIDTTQARPIQYCINGTWYNAGSI"
     gene            645..1178
                     /locus_tag="ISB30_004856"
     CDS             645..1178
                     /locus_tag="ISB30_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP1992379.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSADGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWMFKDGVVV
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1207..1734)
                     /locus_tag="ISB30_004857"
     CDS             complement(1207..1734)
                     /locus_tag="ISB30_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP1992380.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDENNIIVAITRDASTLNPEGFSVVEVPDITANRRTDDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     NRVDTANPEWPQKPE"
     gene            complement(1736..2113)
                     /locus_tag="ISB30_004858"
     CDS             complement(1736..2113)
                     /locus_tag="ISB30_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1992381.1"
                     /translation="MGDNDTGFKQNGDGNLDVYANNVHVMRFVSGSIQSNKTINITGR
                     VNPSDYGNFDSRYVKDVRLGSQQYYGVNNWQTWNFQCPSGHVLSGINVQDTGSNSADN
                     IAGVYYRPVQKYINGTWYNVASV"
BASE COUNT          609 a          458 c          476 g          594 t
ORIGIN      
        1 aatcgacaaa cattcagcgg tggcctttct ggggaactgt ccggcaatgc cgctactgca
       61 acaaagctga aaacagcacg aaaaattgct ggagttggtt ttgatggttc tagcgatatt
      121 tcaattagtg ccaaaaatgt caatgcattt gcactccgac aaacaggtaa tactgttaat
      181 ggtgatacat ccgttggatg gaattgggat agtggtgcat ataacgccat gattggtggt
      241 gcatctgcat taattcttca ctttaatata aatgctggta gctgtcctgc cgtacaattc
      301 cgtgtgaatt ataaaaatgg tggcatttcc tacaggtcgg ctcgtgatgg ttatgggttt
      361 gaattaggtt ggtcagattt ctataccacg acacgaaaac cttcagcggg agatgttggt
      421 gcatatacgc ggacagaatg taactcaagg tttattacag gtattcgcct tggcggtctg
      481 tcatctgttc agacatggaa tggtcccggc tggtctgaca ggtcaggtta tgtcgttacg
      541 ggttcagtta acggaaaccg tgatgaatta attgatacaa cacaggcaag gccaattcag
      601 tattgcatta atgggacgtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa
      661 acattactgc tggcaaccct aaaacaaaag agcaatacca gctaacaaag caatttaaca
      721 tcaaatggct ttattcagct gatggaaaaa actggtatga ggaacaaaag aatttccagc
      781 cagacacttt gaaaatggtc tatgaccata acggcgttat tatttgtatt gaaaaggatg
      841 tttcagcaat taatccggaa ggcgcaagcg tcgttgaatt acctgatatt acagcaaatc
      901 gccgggctga tatttcgggg aaatggatgt tcaaagatgg cgtagtggta aagcgaactt
      961 ataccgagga agagcagagg caacaagcgg aaaatgaaaa gcagagcctg ttgcaacttg
     1021 tcagggataa aactcagcta tgggactcac agctacggct gggcatcatt tccgacgaga
     1081 ataaacaaaa attaaccgag tggatgctct atgcgcagaa agtcgaatcc acagacacct
     1141 ccagcctgcc agtaacattt cccgaacaac ctgaatgaga caaggcccga taacgggcct
     1201 taatttttat tcaggctttt gtggccattc aggatttgcc gtatccacac ggttgaccag
     1261 aacactgtag cgttcccatg cttccagtcg tgtgcgttcc tcgtctgttg ccatattcag
     1321 cctgacagcg cgttccagcg gctggatgac tgattcagct tcggaaagca atgcggcctt
     1381 ttgtgattcg gcctgttgtt gctgttcgtc agccgtataa atccgtttaa ccacagctcc
     1441 gtccttaaac atccacttac cggaatcatc ggtccgacga ttggccgtta tatcgggaac
     1501 ctcgacgacg ctaaaacctt cgggattaag cgtggaggca tctctggtga tggcgacaat
     1561 aatattattt tcatcgtaaa caatctttat ggtgtctggc tgaaagtttt tcacttcctc
     1621 ataccagttt tttccgtcct cagagtaaag ccagataact ccgtgtttct ttgttaactc
     1681 atactgttcc agtgttttag cattacccgc ttttatgttc tttaagtgca tcatattaaa
     1741 cgctcgctac attataccag gtgccattta tatacttttg aacgggtctg taataaacgc
     1801 ccgctatatt atcggcagag ttagaccctg tatcctgaac attaatacca gacaatacat
     1861 gacctgaagg gcactggaaa ttccatgttt gccagttgtt cactccataa tattgctgtg
     1921 aaccaagtcg aacatctttc acataacggg aatcaaagtt accgtaatcc gaggggttaa
     1981 cacgccctgt aatatttatg gttttattac tttgaatgct tccggagaca aagcgcataa
     2041 catggacgtt attagcataa acatccagat taccatcgcc attttgttta aagcccgtgt
     2101 cattatcacc caatacaatc gaattaccgc caagagc
//
LOCUS       DACADF010000309         2076 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.314, whole genome shotgun sequence.
ACCESSION   DACADF010000309 DACADF010000000
VERSION     DACADF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.314"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            11..223
                     /locus_tag="ISB30_004859"
     CDS             11..223
                     /locus_tag="ISB30_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1992382.1"
                     /translation="MITYTTRDGDRLDQICLAVYGRASKTTETVLYQVSNYGVTDMCA
                     VFRAGEKIVLPDIEPEPVKKETQLWD"
     gene            227..1271
                     /locus_tag="ISB30_004860"
                     /pseudo
     CDS             227..1271
                     /locus_tag="ISB30_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287093.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="late control protein"
     gene            1438..1857
                     /locus_tag="ISB30_004861"
     CDS             1438..1857
                     /locus_tag="ISB30_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992383.1"
                     /translation="MVVKNGLACGIMLALVGCATKPVSNEQATPVPEKQIISTALLEK
                     RDGTGEVIIKRDSGFFGSACFSRVYVDGKEVADLGTAQKVVVYPTIGDHVFSAWPKGV
                     CGGGMSEQAGKVTTDRTLMYRIGYGSNGEYGIHPTAF"
BASE COUNT          577 a          435 c          554 g          510 t
ORIGIN      
        1 ggggccccaa atgattacat acactacacg ggacggtgac cgtctcgatc agatctgcct
       61 tgcggtctac gggagagcgt caaaaacgac agagactgtt ttatatcagg tttcgaatta
      121 cggcgtaacg gatatgtgtg ccgtgtttcg tgctggagaa aaaattgttt taccagatat
      181 cgaaccagag ccggttaaaa aagaaacgca attatgggat tgatgaatga gtgattacgt
      241 aaatactggc gtggaggcgt ggaaacctaa tttttatata tctgctgata ataaaaacat
      301 cacggataaa ataagaaaag gtttgatcaa tatcacgctg acagattacg gcggctccag
      361 taaacagaca gacgaactcc gcgtagcaat agtttctgaa actctgaaaa ttccggcccg
      421 cggagtaaaa atcagtattg ggctcggttt cggtaatcag ataattgata aagggatata
      481 catcgttgac ggtgctagca gtggcggtga accccgggta gttgatttca cagcaaaagc
      541 ggctccgatg aacgcagcaa aaggatctcc gacagttcag agcaaaaaga ctcgttcctg
      601 gagcgacgtc actgtcagcg atatcgttgc gacaatagcg agcgataatg ggctaaaacc
      661 acgcgtatca aagcggtttg cagacaatgt tatcacgcaa ctcgatcagg tcggcgagtc
      721 agatatgcac ctgatgtcgc ggctcgcaac gagattcgat gcggtgagta aacccgcggg
      781 cgggtactgg atattcctgc cgcgcggcgc gggcgaatct gttagcggtg ctccgctcca
      841 gcactacacg ctaactcgtc gtgataactc cagctggggc tattcgagaa atggacaaag
      901 tggcgatagt ggtagcggtg gcgaaaatcc ggaaccgacg tatctgatta aatatcacga
      961 cacagcaact gggcagatca aagaattgcg gacaaggtag tggcggcgat cctgtcattg
     1021 aatggccggc agttgagccg tcgctcgacg cagcgaaaga agctgcacca gggctcaagg
     1081 gcggtgcagc taaaaaagag ttctctatga cgtacacgac gccagcgacg ctcgatttag
     1141 tgtcgctgac agcagaatgc aaagtcacaa ctcaaggttt cgggaccgag gaggaccggg
     1201 actggactat caacacactg agtctgacgc tcgctgaaaa cggtttttcg gttcggctgt
     1261 ctctggagta aatgggctcg gcgagccggt ggcggtatga atttagtatt aacagatgtc
     1321 gactgcaaac ggcacctgcg atatttttag gcgtaagccg gagaacgaag agtctctggt
     1381 gctgaacgtt gttatcgtta gatgctgaca tatttttaat attgggagtt ttcaaacatg
     1441 gtcgttaaaa atggtcttgc ctgtgggatt atgcttgctc ttgtcggttg tgctacaaaa
     1501 cctgtctcaa atgaacaagc cactcctgtt cctgaaaaac aaattatcag taccgcgtta
     1561 ctcgaaaaac gagacggtac gggagaggtg atcatcaagc gtgactcggg tttctttgga
     1621 agcgcgtgtt tttcccgtgt ttatgttgat ggtaaagagg tggcagattt aggtacagct
     1681 cagaaagtcg ttgtataccc aacgattggt gatcatgtct tcagcgcgtg gccaaaagga
     1741 gtatgtggtg gtggcatgag cgaacaagcg ggcaaggtga caacagatcg tactttgatg
     1801 tatcgcatag ggtatggatc aaacggtgaa tatggaattc acccaaccgc gttttaattt
     1861 gtgttggggt atgtgagttt ttctcatacc cctttacctc gctttgctcc tgacagtttg
     1921 gtcacataaa ggcgcagaac atcaccattt ttgccaaaaa ccttcagaag acgatgagta
     1981 agacgcgtcg taactatatg attatttgat attcatacac accattatgt ccggataatt
     2041 tgccataatt atttgaattt attgcacaaa gcgacc
//
LOCUS       DACADF010000310         2068 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.315, whole genome shotgun sequence.
ACCESSION   DACADF010000310 DACADF010000000
VERSION     DACADF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.315"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..389
                     /locus_tag="ISB30_004862"
     CDS             <1..389
                     /locus_tag="ISB30_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAP1992384.1"
                     /translation="SQIVNFGQISANSIATYRPKAAFSVSTIKDVAADCTEQFDVVTS
                     FYTTDTLHDDTHLEMGNGLLMRITDQKTQEDIKFNKYKRFTTYIPGQTGAMVTRDYQA
                     ELSQKPGETLVYGPFKKDLIVKINYN"
     gene            complement(443..1096)
                     /gene="ribB"
                     /locus_tag="ISB30_004863"
     CDS             complement(443..1096)
                     /gene="ribB"
                     /locus_tag="ISB30_004863"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAP1992385.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(1200..1348)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1470..1769
                     /locus_tag="ISB30_004864"
     CDS             1470..1769
                     /locus_tag="ISB30_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HAP1992386.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNPAAELQSPPAIPPVDKAE"
     gene            complement(1804..2004)
                     /gene="glgS"
                     /locus_tag="ISB30_004865"
     CDS             complement(1804..2004)
                     /gene="glgS"
                     /locus_tag="ISB30_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAP1992387.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT          579 a          529 c          457 g          503 t
ORIGIN      
        1 aaagccagat cgttaatttc gggcaaatct ctgcaaactc tattgctact tatcgaccca
       61 aagctgcatt tagcgtatct actataaaag atgttgctgc cgactgtacc gagcaatttg
      121 atgttgtcac cagtttttat acgacagata cgttacatga tgatacgcat ctggaaatgg
      181 gtaatggttt gttaatgcgt attactgatc aaaaaacaca agaagacatt aagtttaata
      241 agtacaaacg cttcaccaca tatattccag gtcagactgg agcgatggtt acccgtgatt
      301 accaggctga actttcacaa aaaccggggg agactctcgt ttatgggcct ttcaaaaaag
      361 atctcatcgt aaaaataaac tataactgaa cagcaaaatg ccgggaacac cccggcattt
      421 tcatatttag tatttcacgt cattagctgg ctttacgctc atgtgcctga cggtatgcca
      481 ccaggtcttc aatagtcacg agcgccatat tgtgtttatt cgcaaactca atgcactctg
      541 gtgcacgcgc catcgtgcca tcgtcattag tcagctcaca cagcacacca gctggtttaa
      601 agcctgccag cgtcatcaga tcgatagtcg cttcagtatg accgccacgc gtcagtacac
      661 cacctgcctg cgcgcgaagt gggaaaacgt ggcctgggcg attcagatct gaaggttttg
      721 cgccatcggc aatcgctgcg cgaacggtcg taatacggtc agcggcagaa acaccggtag
      781 tcacaccttc agctgcttca atggtcacgg taaaaccggt gccataggcg ctggtgttat
      841 tttctaccat cattggcaga tcgagttgtt tacggcgatc gtcagtaatg cacaggcaaa
      901 caataccact cccgtggcga atggtcagcg ccatctgctc aacggtcatg gtttctgccg
      961 ggaagatcat atcgccttcg ttttcacggt cttcatcatc aagcaccatc acaccgcgtc
     1021 cttcacgcag cgcagccagt gcattttcaa cacgttcgaa aggcgtacca aaagaggaaa
     1081 gtagcgtctg attcatggta aaaaaacctc actaaaatta tggttaccag aatcagggca
     1141 gtcttaggag tggcggcata tagccaaaat aacgtgagcg ggtccatgcc cgacagaatc
     1201 gttactctct cccatccgga ctctaaccgt cggccccgga attacaccgg atctgctgtc
     1261 ctttgagttc gcacccaaag cgctcgcggg ctttcaactg agttgattta ccgccggtgg
     1321 ggaatttcgc cccgccctga gaataagcgg attcactata acgctaatga ttagcgccag
     1381 caacgcatag cttcacataa ttctggttta tgacttaccc ttatcgcact acaatggcac
     1441 tcaacaccta tcagtacagg gaaactacaa tgattgaccc gaaaaaaatt gagcaaatcg
     1501 ctcgccaggt tcacgaatca atgcctaaag gaatcaggga gttcggggaa gatgtggaga
     1561 aaaaaatccg ccaaacccta caagcgcagc tgacgcgtct cgatctggta agccgcgaag
     1621 aattcgacgt tcagacgcaa gtcctgttac ggactcgtga aaaactggcg ttactggagc
     1681 agcgcatcag cgaactggaa gcgcgcaata atccggcagc agaacttcaa tctcctccgg
     1741 ctatcccgcc agttgataaa gcagaataaa gcaccacaaa aacgggccag acggcccgta
     1801 atatcagtgt tctaattcaa gttctctggt ttgcatcatc tgttggcaat accaggcata
     1861 acgtgcgcaa aaccaggttc tatgttcttc atccatgtta ccagtaacgg ccagaatatc
     1921 gaccgggcga ccttctgcgt gcattctggc aaaactacgc gcgagaaaat cgaaattatt
     1981 taaagaatta agactatgat ccataagcac atcctccggt tattgcatac cttttaaggc
     2041 catatgcttt ttctgatttc agtattcc
//
LOCUS       DACADF010000311         2066 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.316, whole genome shotgun sequence.
ACCESSION   DACADF010000311 DACADF010000000
VERSION     DACADF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.316"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..1765
                     /gene="sat"
                     /locus_tag="ISB30_004866"
     CDS             <1..1765
                     /gene="sat"
                     /locus_tag="ISB30_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAP1992388.1"
                     /translation="NSQIAIGDQAVVTLNGATFLNNTPISIDKGAKVIAQNSMFTTKG
                     IDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTGNIYADDAA
                     TITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAIWHLNSQSS
                     INRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQLVVNKSLSGKNN
                     LLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTPVIEQKNDTDKAT
                     WTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDINGESGAWARIMS
                     GTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGSDAFSGETKSVGA
                     GLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYAGAEVGYRYHVTD
                     SAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKV
                     TARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAEIRDNLRFGLEFE
                     KSAFGKYNVDNAINANFRYSF"
BASE COUNT          590 a          468 c          492 g          516 t
ORIGIN      
        1 taacagccaa atagccatag gtgatcaagc tgtagttaca cttaatggtg caacctttct
       61 gaataatact cctataagta tagataaagg agcaaaagtt atagcacaaa attccatgtt
      121 cacaacaaaa ggtattgata tctccggtga gctgactatg atgggaatcc ctgaacagaa
      181 tagtaaaact gtaacgccgg gtctccacta cgctgctgat ggattcaggc tgagtggtgg
      241 aaatgcaaat ttcattgcca gaaatatggc atctgtcacc ggaaatattt atgctgatga
      301 tgcagcaacc attactctgg gacagcctga aactgaaaca ccgactatat cgtctgctta
      361 tcaggcatgg gcagagactc ttttgtatgg ctttgatacc gcctatcgag gcgcaataac
      421 agcccccaaa gctacagtta gcatgaataa tgcgatctgg catctaaata gccagtcatc
      481 aattaatcgt ctagaaacaa aagacagtat ggtgcgtttt actggtgata atgggaagtt
      541 tacaaccctt acagtggaca accttactat agatgacagt gcatttgtgc tgcgtgcaaa
      601 tctggcccaa gcagatcagc ttgttgtcaa taaatcgttg tctggtaaaa acaaccttct
      661 gttagtcgac ttcattgaga aaaatggaaa cagcaacgga ctgaatatcg atctggtcag
      721 cgcaccaaaa ggaactgcag tagatgtctt taaagctacg actcggagta ttggcttcag
      781 tgatgtaaca ccggttatcg agcaaaagaa cgatacagac aaagcaacat ggactctgat
      841 cggctataaa tctgtggcca acgccgatgc ggctaaaaag gcaacattac tgatgtcagg
      901 cggctataaa gccttccttg ctgaggtcaa caaccttaac aaacgtatgg gtgatctgcg
      961 tgacattaac ggtgagtccg gtgcatgggc ccgaatcatg agcggaaccg ggtctgccgg
     1021 cggtggattc agtgacaact acacccacgt tcaggtcggt gcggataaca aacatgaact
     1081 cgatggcctt gacctcttca ccggggtgac catgacctat accgacagcc atgcaggcag
     1141 tgatgccttc agtggtgaaa cgaagtctgt gggtgccggt ctctatgcct ctgccatgtt
     1201 tgagtccgga gcatatatcg acctcatcgg taagtacgtt caccatgaca acgagtatac
     1261 cgcaactttc gccggccttg gcaccagaga ctacagctcc cactcctggt atgccggtgc
     1321 ggaagtcggt taccgttacc atgtaactga ctctgcatgg attgagccgc aggcggaact
     1381 tgtttacggt gctgtatccg ggaaacagtt ctcctggaag gaccagggaa tgaacctcac
     1441 catgaaggat aaggacttta atccgctgat tgggcgtacc ggtgttgatg tgggtaaatc
     1501 cttctcgggt aaggactgga aagtcacagc ccgcgccggc cttggctacc agtttgacct
     1561 gtttgccaac ggtgaaaccg tactgcgtga tgcgtccggt gagaaacgta tcaaaggtga
     1621 aaaagacggt cgtatgctca tgaatgttgg tctcaacgct gaaattcgcg ataatcttcg
     1681 cttcggtctt gagtttgaga aatcggcatt tggtaaatac aacgtggata acgcgatcaa
     1741 cgccaacttc cgttactctt tctgataaca gcccgggccg cgtttgcggc ccttcttcta
     1801 ccggagagaa tatgtattac cctgtgacag actatatcgc tcttgctctc attattagct
     1861 ttctttttct gacattattt atctgcctgt tatgccttaa acatgagcga ataaaaaaag
     1921 aaactatcag gcaaaaaaat gcacatattc tggatcatgg ctggaatgca actgaggtag
     1981 cctgagttta acggacactc cttcctgaaa tagaatggca tcagaaggag ctaataatga
     2041 gcagaaaaac ccaacgttac tctaaa
//
LOCUS       DACADF010000312         2061 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.317, whole genome shotgun sequence.
ACCESSION   DACADF010000312 DACADF010000000
VERSION     DACADF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.317"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            66..1199
                     /gene="ompN"
                     /locus_tag="ISB30_004867"
     CDS             66..1199
                     /gene="ompN"
                     /locus_tag="ISB30_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HAP1992389.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1340..1774
                     /gene="uspF"
                     /locus_tag="ISB30_004868"
     CDS             1340..1774
                     /gene="uspF"
                     /locus_tag="ISB30_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAP1992390.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1863..>2061
                     /locus_tag="ISB30_004869"
     CDS             1863..>2061
                     /locus_tag="ISB30_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP1992391.1"
                     /translation="MNYALIIAIIISFPCALLSVFLVLKGWALIGDAMSHAMFPGIVL
                     VWIIGMPLGIGAFIVGLSCAIA"
BASE COUNT          553 a          459 c          473 g          576 t
ORIGIN      
        1 ttaggatgtt ttcactcccc gaaatgggga tgaaaggcaa ataaaataac taaaggattt
       61 attcaatgaa aagcaaagta ctggcactct taattcctgc cctgctcgct gcaggtgctg
      121 cacatgcagc cgaagtttat aataaagacg gcaacaaatt ggatctgtat ggcaaagttg
      181 atggcctgca ttatttttct gataattcag cgaaagatgg cgaccagagc tatgctcgtc
      241 tgggttttaa aggcgaaacc caaattaacg atcaactcac tggctacggt caatgggaat
      301 acaatattca ggcaaacaac accgaatctt caacaaacca gtcatggacc cgtctggcat
      361 ttgccggact gaaatttgca gattacggtt ctttcgatta cggacgtaat tatggcgtaa
      421 tgtacgacat cgaaggctgg actgatatgc tacctgaatt tggcggtgac tcttatacca
      481 atgcagacaa ctttatgact ggtcgtgcca atggcgtcgc gacttatcgt aatactgatt
      541 tcttcggtct ggtaaatggt ctgaacttcg cggtgcagta tcaaggtaac aacgaaggtg
      601 ccagtaatgg tcaggaaggc accaacaacg gacgtgatgt tcgccatgaa aacggtgacg
      661 gctggggtct ttccacaaca tatgatttag gcatgggctt tagcgctggt gcggcataca
      721 cctcttctga ccgcaccaat gaccaggtta accatactgc ggcgggtggt gataaagcag
      781 acgcgtggac tgctgggcta aaatacgatg ctaacaatat ttacctggca accatgtatt
      841 cagaaacgcg taatatgacc ccgtttggcg acagcgatta tgctgttgca aacaaaaccc
      901 agaactttga agtcactgca cagtaccagt ttgattttgg tctgcgtccg gcagtctctt
      961 tcctgatgtc taaaggccgt gacctgcacg ctgccggtgg tgcagacaat ccggcaggtg
     1021 ttgatgataa agatctggtt aaatacgccg atgttggcgc gacttactat ttcaataaaa
     1081 acatgtccac ctatgttgac tataaaatca acctgttgga tgaagatgac agtttctacg
     1141 ctgccaacgg catctctacc gatgatattg tcgctttagg tctggtttat cagttctaaa
     1201 tccccctgcc cgctgttttg gcgggctttt tctgcttatt cttcctcctt tgatctaaat
     1261 taaaaatgtg aactccgtca ttacacaaaa agtgtcatct ggcgttacac tttatgcgga
     1321 tactaaaaca ggaggtttta tgaacagaac gattcttgtc cctatcgata tttccgattc
     1381 agaattaact caacgcgtga ttagccacgt tgaggcagag gcaaagattg atgatgcaga
     1441 ggttcatttc ctgacggtaa taccttcgct gccctactat gcctctctgg gtttagcgta
     1501 ttccgcagaa ttaccggcaa tggatgacct gaaagcggaa gccaaatcgc aactggaaga
     1561 gatcattaaa aaatttaaac tgccaaccga cagagtgcat gtccatgttg aggaaggctc
     1621 gcccaaagac cgcattctgg aattggcgaa gaagatcccc gctcatatga tcatcattgc
     1681 ttcccatcga ccggatatca ccacttatct gctcggttct aacgccgcag ctgtagtgcg
     1741 tcacgcagag tgctccgtgc tggttgtgcg ctgacactaa cgcccgcaca ttgctgcggg
     1801 ctttttgatt catttcgcaa atgtgctgat attttcccct ctaatcatta caatttaaat
     1861 ttatgaatta tgcattgata atcgctatta taatatcgtt cccctgtgca ttattatcag
     1921 tttttctggt attaaaaggc tgggcgctaa taggagatgc aatgagccac gcaatgtttc
     1981 ctggcatagt tctagtctgg attataggta tgccgctggg aattggtgca tttattgtag
     2041 gactgtcttg tgcaattgca a
//
LOCUS       DACADF010000313         2035 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.318, whole genome shotgun sequence.
ACCESSION   DACADF010000313 DACADF010000000
VERSION     DACADF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.318"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            61..654
                     /locus_tag="ISB30_004870"
     CDS             61..654
                     /locus_tag="ISB30_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992392.1"
                     /translation="MTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSVMTHVRLFD
                     SDMTFFGKIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYG
                     QTNGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIA
                     WKFYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
     gene            675..>2035
                     /locus_tag="ISB30_004871"
     CDS             675..>2035
                     /locus_tag="ISB30_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP1992393.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGSDRTDQPQAQDMGFDYSLVNTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAVGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYLYNLKSDR
                     YETL"
BASE COUNT          574 a          399 c          504 g          558 t
ORIGIN      
        1 gcgggttatg cagactggaa tagtggattt gtaaacactc accgtggtga agtatggaaa
       61 gtgactgcgg attttggggt aaattttaaa gaagcagaat tttactcatt ttatgaaagt
      121 aatgtactca atcatgctgt agcagggcga aatcatacgg tttcagtaat gacgcatgtc
      181 agactctttg actctgatat gacattcttt ggcaaaattt atggccaatg ggataactca
      241 tggggtgacg atctggacat gttttatgga ttcggttacc tcggctggaa cggcgagtgg
      301 ggctttttta aaccgtatat tggattacat aatcaatctg gtgactacgt atcagctaaa
      361 tatggtcaaa cgaatggttg gaatggttat gttgttggct ggacagcagt attaccattt
      421 acgttatttg acgaaaaatt tgttttatct aactggaatg aaatagaact ggacaggaac
      481 gatgcttaca cggagcagca atttggccgg aacgggttaa atggcggttt aactattgcc
      541 tggaagttct atcctcgctg gaaagccagt gtgacgtggc gttatttcga taataagctg
      601 ggctacgatg gctttggcga tcaaatgatt tatatgcttg gttatgattt ctaatttttt
      661 atcagggaat aattatgcag aaaacgttaa tggccagttt gatcggcctt gcagtttgca
      721 cagggaatgc ttttagtcct gccttagccg cagaggctaa acaacctaat ttagtcatta
      781 ttatggcgga tgatttaggt tatggcgatt tagcaacata tggtcatcag atcgttaaaa
      841 cacctaatat cgacaggctt gcccaggaag gggtcaaatt tactgactac tatgcccccg
      901 ctcctttaag ttcaccttca cgcgcagggc tattaaccgg ccggatgcca tttcgtactg
      961 gaattcgctc atggattcct tcaggcaaag atgttgcctt agggcgtaac gaactcacga
     1021 ttgctaatct actcaaagcg caagggtacg acacggcaat gatgggtaag ctgcatctga
     1081 atgcaggcag cgatcgcacc gatcagccac aagcacaaga tatgggcttt gattactcac
     1141 tggttaatac ggcgggcttt gttaccgacg ccacgctgga taacgctaaa gaacgcccgc
     1201 gttatggcat ggtttacccg acaggctggc tacgtaacgg gcaacccact ccacgagccg
     1261 ataaaatgag cggtgagtat gtcagttcgg aagtcgtcaa ctggctggat aacaaaaagg
     1321 acagcaagcc tttcttcctc tatgttgctt ttaccgaagt gcatagcccc ctggcttcgc
     1381 ccaaaaaata cctcgatatg tactcacaat atatgagcgc gtatcagaag cagcatcctg
     1441 atttatttta tggcgactgg gcagacaaac cctggcgtgg tgtgggggaa tattatgcca
     1501 atatcagcta tctggatgca caggttggaa aagtgctgga taaaatcaaa gctgtgggtg
     1561 aagaagataa cacaatcgtt atttttacca gtgataacgg tccggtaacg cgtgaagcgc
     1621 gcaaagtgta tgagctgaat ttggcagggg aaacggatgg attacgcggt cgcaaggata
     1681 acctttggga aggcggaatt cgtgttccag ccattattaa atatggtaaa catctaccac
     1741 agggaatggt ttcagataca cccgtttatg gtctggactg gatgcctact ttagcgaaaa
     1801 tgatgaactt caaattacct acagaccgta ctttcgatgg tgaatcgctg gttcctgttc
     1861 ttgagcaaaa agcattgaaa cgcgaaaagc cattaatttt cgggattgat atgccattcc
     1921 aggatgatcc aaccgatgaa tgggcgatcc gtgatggtga ctggaagatg attatcgatc
     1981 gcaataataa accgaaatat ctctacaatc tgaaatctga tcgttatgaa acact
//
LOCUS       DACADF010000314         2031 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.319, whole genome shotgun sequence.
ACCESSION   DACADF010000314 DACADF010000000
VERSION     DACADF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.319"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            192..530
                     /locus_tag="ISB30_004872"
                     /pseudo
     CDS             192..530
                     /locus_tag="ISB30_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            716..1930
                     /locus_tag="ISB30_004873"
     CDS             716..1930
                     /locus_tag="ISB30_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP1992394.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
BASE COUNT          619 a          434 c          430 g          548 t
ORIGIN      
        1 gtcctgatac aaatttttag agtagtcatt agtgaacaga taattgttaa cttagaacca
       61 gtaaaatgca aacgacgcac cacgctggga aaatcgctgc tattcagcag agttttgtac
      121 cgagaattgt ggccagccac ttattgctct cgcgtaagca ggtatcccca tcgtttacca
      181 gtccagcaca tcttgtggtc aggcagaaga agagcaaact gttactggac tcactgcatg
      241 catggtggag aaaaatgcca cgctatccaa aaatccagac tgggtgaatg gttcagctgt
      301 gtactgaatc agtggggcgc actctgttat tacagtgttg acggtcaggc ggaggcagac
      361 ataatgccgc ggaaaaagcc ctttgtgcca tctgtctcgg aaataaaaac tttatgttct
      421 tcggcggcga gcgcagtgca ctgttgtatg ggcttatcgg cacttgcctt ctgaacgaca
      481 ttgatccgga agcgtatctg cgccatatcc tgagcgtact gccggaataa ccttccaacc
      541 gtattgatga actcctgcca tggaacgtag ttctcaccaa taaataagca tcaatatgat
      601 tctccattaa cgcttacctt ttctcaggct atttttgctt ttccccgcca ctttatggtg
      661 attatcacat caatatacat cgatagatat taacgtaact gaagtgaggt gaagcatgaa
      721 atttgaacgc catcatgaaa tattgaaacg actcagtaaa ttcggttctg taaaagtatc
      781 cgatctgtcc aatagcctca atgtaacgaa agaaactatt cgatcagatc ttaatgaact
      841 tgccaggctt ggctatctga ctcgatgtca tggcggtgct tttatcgtcc tagactcgct
      901 tgatactatt gcgaaaaatg aaatcgctta tgcgcttgaa aattatgata cagcccaggg
      961 aataaaaaaa ggccactcaa ctatgaaaag tcaagtatgc gttattggtt cgtttaatgt
     1021 tgatatcatc agttatttgc cgaggcttcc cactatcgga gaatcgcttc tggcaagtaa
     1081 ttttatattt tcaccggggg gaaaaggctg taaccaagcg ctggcagcaa gctttgccga
     1141 tactgatgtt tatttcataa caaaagtagg tacagatcac tttagtgatt atgcgatcaa
     1201 ctttatgaat tcgtctaaga tttataaaag tataatttat caaacgaaag aaacgcaaac
     1261 cggaactgcc acgatccttg ttaatgaagg tactggcgat aatgttattg cgatctatcc
     1321 cggtgccaac atgacaatgt cgtccgatga aataactatt cagaaagaag cgattattaa
     1381 ttctgatgtc atacttctgc agctcgaaac aaattatacc gctctgcaac aagcgatcac
     1441 attggcgcaa aaaaacagta tcccggtcat tattaacccg gcaccctata atgatatcgt
     1501 caacgaactt attcaggatg ttgattatat cacccccaat gaaacagaag ctggtttact
     1561 gtccggcata gacgtacacg atcttgaatc agctaaacga gctgcagaag ctatccacaa
     1621 taaaggggta aaaaacacgg ttatcacgct cggtagcaag ggctcgctcg catttgatgg
     1681 caaaaaattc attcattcgc cagcgtttcc agcggtcgtt aaaaatacgg caggagccgg
     1741 ggatgctttt aacggcgctc tggcatcagg acttgcgaaa ggaaaatcac tggaatcagc
     1801 actgtgttat gccagcgcct ttgcatccct agcggtggag acaagcaatg cttccgacat
     1861 gcctgaacat gaatctgtga tacatcgaat tcagagtata cattatcagc aaactatttt
     1921 tacgcattaa gaagggacag agaatgaata acaccaaaaa atcactaaaa gtgctcttta
     1981 ttggggaatc ctggcatatt catatgattc attcaaaagg atatgacagt t
//
LOCUS       DACADF010000315         2025 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.320, whole genome shotgun sequence.
ACCESSION   DACADF010000315 DACADF010000000
VERSION     DACADF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.320"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..919)
                     /locus_tag="ISB30_004874"
     CDS             complement(<1..919)
                     /locus_tag="ISB30_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1992395.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQHLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     IPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKI"
     gene            1076..1996
                     /locus_tag="ISB30_004875"
     CDS             1076..1996
                     /locus_tag="ISB30_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1992396.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFNASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPLMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT          588 a          453 c          431 g          553 t
ORIGIN      
        1 ctatttttac tcttttctcc accataaaat caataaaagc acgaacttta gagggcattc
       61 tcacccgaga tgaaacatat atataaaatc caggaaatga aacggcccaa tcctcaagaa
      121 tcggctgtaa cttgcccgca ttgatatgtt cagcgattgc aaaatccaga tgttgaatga
      181 tacccgttcc attcagcgcc attcgtatca tgctttcgtc atcattagaa atatacttac
      241 ctgacacctc tatttcgaac tcttcattac cccccacaac attcgaaaac tcccatgctg
      301 agagtgcacc gctactttta tgccgcaacc gaagacaatt atggttactc aattcctgag
      361 gagtttcagg aataccgtgt tcttttaaat aatctggcga tgccacagta accagtttaa
      421 taagcggaga aacaggaatg gcagtcatat gctcatccag tccttgctca agacgtatgc
      481 ccacatcaca gccgtccgca ataatgttag ataaaccgtc atccataatg agttctatct
      541 ttgttttggg gtaacgagta agaaactcgc caatatgagg ctcaacaagc atccttgctg
      601 caacacggga ggaatttatt ctcaacagtc ctgtaggttc aagttgggta tcaccgacat
      661 ctttcaacgc atcatcaatt tgccccaaag ccgacaccag cacttcatac agatgctgtc
      721 cttcctcagt aagcgacata ttgcgggttg tacggtatat cagcttggtg ttaagacgtt
      781 cctcaagaga ttttaaattc tgggacaatg cggcccgcgt aatacccatt tcagaggctg
      841 ctctggaaaa actgccggca cgaacaatca gcgcaaacca ggcaagggaa ggcaaaattg
      901 aaggctctat tttcatcatt gtaaacctta acttaacact gtgatagata ttagaccatt
      961 gatgataacc aatacaagtc atactattac gccgttcgcg atatgaacac gaatgccagt
     1021 tgcggataat agaaatgata tttaaatctg tttaggcaaa tccctggagc ttgaaatgaa
     1081 aactgtttca ataaaacaca cttactggga tatagctgct gatatctatt tccctgacga
     1141 ttttaatagt gaaaaaaagt atcctgccat tatcagtgcc catccaattg gaagctgcaa
     1201 agaacagaca tcgggtagtg tgtatggtgc cgccctggca aaagctggct ttatcgtcat
     1261 tgcttttaat gccagttttc agggaagcag cggtggagaa ccgcgttatc tggaagatcc
     1321 aacaatgcga gtaaaagact tcagcattgt cgttgattac cttaccacct taccttacgt
     1381 tgatgccggg cgtattggtg tactgggtat ttgtggtgga ggtggatatg ccattaatgc
     1441 cgcaatgaca gaacgtcgga ttaaagccat tggtactgtc acaggtgcaa actacgggcg
     1501 tcttatgcgt gagggattta cagcgtttaa tcctatcgga gcactcgaag caatggcgca
     1561 acaaagaaca gacgaagcta acggcgcaaa acttcgcgta gatgatttgc ttccttcttc
     1621 tccggaagca gctcttgaag cgggattaac agaaatagac ctttttgaag caacagaata
     1681 ttatcgttcg ccacgaggct gtgcgcctaa tggggtaaat cgttcgctat tttcacatca
     1741 gactgttgcc gtgggctggg atgcttttca tcttgcagaa gtcctgttaa ctcaaccact
     1801 aatggttgta gtaggcgata gagtgggcgc ttttggtgcc tatcgtgacg gctgcgaaat
     1861 tattggtcgt gcagcctcaa aacataaaga gttggtcgtg gttgaaggtt attcacatta
     1921 cgacctgtat gataagccag aacctgtgaa acaggcgctc gataaattaa ttccttttta
     1981 caaaacacat ttgtaataac cttgcaggga gattcattat ctccc
//
LOCUS       DACADF010000316         1989 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.321, whole genome shotgun sequence.
ACCESSION   DACADF010000316 DACADF010000000
VERSION     DACADF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.321"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..1291)
                     /gene="mmuP"
                     /locus_tag="ISB30_004876"
     CDS             complement(<1..1291)
                     /gene="mmuP"
                     /locus_tag="ISB30_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methylmethionine permease"
                     /protein_id="HAP1992397.1"
                     /translation="MQTTQQNAPLKRTMKTRHLIMLSLGGVIGTGLFFNTGYIISTTG
                     AAGTLLAYLIGALVVWLVMQCLGELSVAMPETGAFHVYAARYLGPATGYTVAWLYWLT
                     WTVALGSSFTAAGFCMQYWFPQVPVWVWCVVFCAIIFGLNVISTRFFAEGEFWFSLVK
                     VVTIIAFIILGGAAIFGFIPMQDGSPAPGLSNITAEGWFPHGGLPILMTMVAVNFAFS
                     GTELIGIAAGETENPRKVIPVAIRTTIARLIIFFIGTVFVLAALIPMQQAGVEKSPFV
                     LVFEKVGIPYAADIFNFVILTAILSAANSGLYASGRMLWSLSNERTLPACFARVTKNG
                     VPLTALSVSMLGGVLALFSSVVAPDTVFVALSAISGFAVVAVWLSICASHFVFRRRHL
                     QQGKALSELHYRAPWYPLVPVLGFVLCLVACVGLAFDP"
     gene            1499..>1989
                     /locus_tag="ISB30_004877"
     CDS             1499..>1989
                     /locus_tag="ISB30_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5B family transposase"
                     /protein_id="HAP1992398.1"
                     /translation="MFVIWSHRTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTISRWLAEAGVMMTQGTLVDATIIE
                     APSS"
BASE COUNT          543 a          591 c          490 g          365 t
ORIGIN      
        1 ctggatcgaa tgccagccca acacaggcca ccaggcacag cacaaaacct aatactggca
       61 ccagcggata ccacggcgcg cgataatgta attcactcaa tgccttacct tgttgcagat
      121 gacggcgacg aaaaacaaaa tgcgaggcgc agatactcag ccacaccgct accaccgcaa
      181 acccggagat tgccgacagc gcaacaaata ccgtgtccgg ggccaccacg ctggaaaaca
      241 gcgccagcac accaccgagc atactgaccg acagcgccgt cagtggcacg ccgtttttcg
      301 ttactcgcgc aaaacaggcc ggtagcgtac gttcattcga caacgaccac agcatgcgcc
      361 cggaggcata taacccggag ttcgctgcag aaagaatagc cgtcaggatc acgaagttaa
      421 aaatatcagc ggcgtacggg atccctactt tctcaaatac cagcacaaac gggcttttct
      481 ccacgcccgc ctgctgcatc gggatcagcg ctgccagcac aaacacggtg ccgataaaga
      541 aaataatcag tcgcgcgatg gtagtacgaa tcgctaccgg gataactttg cgcgggtttt
      601 ccgtttcacc ggcggcaatg ccgataagct cggtacccga aaaagcaaag ttcactgcca
      661 ccatagtcat caaaatcggt aagccaccgt gcgggaacca gccttctgcc gtgatattac
      721 tcagccccgg cgcgggcgag ccatcctgca tcggaataaa gccgaaaatc gccgccccac
      781 cgaggatgat aaaggcgatg atagtgacca ctttgaccag cgagaaccag aactcccctt
      841 cggcaaaaaa gcgcgtggag ataacattca gaccaaaaat aatcgcgcag aacaccacgc
      901 accagaccca taccggcacc tgtggaaacc agtactgcat acagaatcca gcggcggtaa
      961 agctcgaacc cagcgccacg gtccaggtca gccagtaaag ccaggccacg gtatacccgg
     1021 tagccggacc aagatagcgc gcggcataaa cgtgaaacgc tccggtctcc ggcatcgcga
     1081 ccgacagctc gcccagacac tgcataacca gccagaccac cagcgcacca atcagatagg
     1141 ccagcagcgt tcccgccgct ccagtggtgg aaatgatgta cccggtattg aagaataatc
     1201 ctgtgccaat cacgccgccc aaggaaagca taatcaggtg acgcgttttc attgtgcgct
     1261 tcagtggcgc attttgttgt gttgtttgca tataaccttc taaacgtatg gacatctaaa
     1321 catccaaaga aggtttgtta taccgcgttt gttaacagaa tgcaaagtca accctgcacc
     1381 agacgcagat gcccgcgccg ggcgcttaat agcgggtggg gattaataat cagatacaaa
     1441 aagggccgcc agctcataac gcctccggaa ggtgcgaaca agtccctgat atgagatcat
     1501 gtttgtcatc tggagccata gaacagggtt catcatgagt catcaactta ccttcgccga
     1561 cagtgaattc agcagtaagc gccgtcagac cagaaaagag attttcttgt cccgcatgga
     1621 gcagattctg ccatggcaaa acatggtgga agtcatcgag ccgttttacc ccaaggctgg
     1681 taatggccgg cgaccttatc cgctggaaac catgctacgc attcactgca tgcagcattg
     1741 gtacaacctg agcgatggcg cgatggaaga tgctctgtac gaaatcgcct ccatgcgtct
     1801 gtttgcccgg ttatccctgg atagcgcctt gccggaccgc accaccatca tgaatttccg
     1861 ccacctgctg gagcagcatc aactggcccg ccaattgttc aagaccatca gtcgctggct
     1921 ggccgaagca ggcgtcatga tgactcaagg caccttggtc gatgccacca tcattgaggc
     1981 acccagctc
//
LOCUS       DACADF010000317         1946 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.322, whole genome shotgun sequence.
ACCESSION   DACADF010000317 DACADF010000000
VERSION     DACADF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.322"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            34..1257
                     /locus_tag="ISB30_004878"
     CDS             34..1257
                     /locus_tag="ISB30_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAP1992399.1"
                     /translation="MGTIQKIRTDVNVLYAAIHRIEWLFETFSSVCLSFSGGKDSTVL
                     LHLTADVARRKKRRFSVLFIDWEAQYQCTIAHILKMREMYRDVTETFYWVALPLTTVN
                     GVSQFQPEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAGNRC
                     GVAILTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTLYPLYDWKARDIW
                     IYNARTRAIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCER
                     VSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAVY
                     LRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYER
                     YLQRMKERRKEWGIL"
     gene            1242..1886
                     /locus_tag="ISB30_004879"
     CDS             1242..1886
                     /locus_tag="ISB30_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAP1992400.1"
                     /translation="MGDSVTPEVEVLSNMIRQYFSQERSEEETIRALNHLRRVLHEVS
                     PFAQEPVDCVLWVKADEVVANDYNPNVMSSSEKKLLKHSLEQDGFTQPVVVSEEKEHY
                     LVVDGFHRQLLGRKADTRKRLKGWLPVTCINPERKGQASRIAATIRHNRARGKHQITS
                     MSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELFQEENFSPSWTVR"
BASE COUNT          488 a          408 c          550 g          500 t
ORIGIN      
        1 attactgcca agatagttta tactaggata tgtatgggaa ccatacagaa gataagaaca
       61 gatgttaatg tattatatgc ggccattcac cgtattgagt ggttgtttga gacattttca
      121 tctgtttgtt tgtctttttc tggaggaaaa gactctactg tgctgctcca tcttacggcc
      181 gatgtggctc gcaggaagaa acgtcgtttc tctgtattat tcattgactg ggaagctcag
      241 taccagtgca caattgcaca cattctgaag atgagggaga tgtatcggga tgtgacggag
      301 actttttact gggtggcact ccccctgact acggtaaacg gtgtctctca gtttcagccg
      361 gaatggatat gctgggaacc aggtgtggag tgggttcgtc agccaccaga tgacgctatt
      421 acagatatgt cgtatttccc attttatcgg tatgccatga cgtttgaaga atttgttccg
      481 gcattttctt cctggtttgc cggtaaccgg tgtggagtgg caatactgac tggtgttcgt
      541 gctgatgaat cgctcaatcg ctttatggga ctggtgtctc agcgcaaact gagatatgca
      601 gatgataaac cctggaccac agcgtcacct gaagggtttt attacacctt gtatccgttg
      661 tatgactgga aagcccgtga tatatggata tataacgcca gaacccgagc tatctacaat
      721 cccctgtatg acctgatgta ccgtgccggc gtgccgttac gcaacatgcg ggtctgtgag
      781 ccttttggcc cggaacagcg taagggactg tggctttacc atgttctgga gccggaaact
      841 tgggccagga tgtgtgagcg ggtgtcgggt gctgccagcg gggcgcttta tgccaatgag
      901 agcggtgcct attttgccct gcgtaagcgt atcacgaagc cacctcatca tacctggcgt
      961 agctatgcga tgttcctgct ggatgtgatg ccggaaagaa cggcagaaca ttaccgtaat
     1021 aaaattgctg tctacctgcg ctggtatcag acgcggggct tcccggatga catcccggat
     1081 gaacaggaga atgacctggg gagccgggat atcccgtctt ggcgacgtat ctgtaagaca
     1141 ctcataaaga atgatttctg gtgccggacc ctctccttca gtccgaacaa accccggcac
     1201 tatgaacgtt atctgcagcg tatgaaagaa aggaggaagg aatgggggat tctgtgacac
     1261 ctgaggtgga agtattgagc aacatgattc gacagtactt cagtcaggaa cgatctgaag
     1321 aagagaccat acgagccctt aatcatctgc gcagggtgtt gcatgaggtc agcccttttg
     1381 cacaggagcc tgtggactgt gtgttgtggg tgaaagcgga tgaggtggtg gccaacgatt
     1441 acaatcccaa tgtcatgtct tccagtgaaa aaaaactgct taaacattca ctggagcagg
     1501 atggattcac gcagcctgtg gtggtttcgg aagagaagga gcattatctg gtcgtggatg
     1561 gttttcatcg tcagttgctg ggcaggaaag cggatacgag aaaacgtctg aagggttggc
     1621 tgccagtgac ctgtattaat ccggaaagaa agggacaggc atcacgtatt gccgcaacca
     1681 tacggcataa ccgtgccagg ggaaaacacc agataacgtc aatgtctgac attgtcaggg
     1741 acctttcgcg tctgggctgg acagatgagc gtattggcac agagttgggc atggaccagg
     1801 atgaagtgct gaggctgaaa cagattagcg ggctgacaga gctgtttcag gaagagaatt
     1861 tcagcccttc atggacggtt cgttgatatt gtaaaatatg atagttggag taaggggcaa
     1921 tagaaggaga atatttatcc cacctt
//
LOCUS       DACADF010000318         1946 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.323, whole genome shotgun sequence.
ACCESSION   DACADF010000318 DACADF010000000
VERSION     DACADF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.323"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..1194
                     /locus_tag="ISB30_004880"
     CDS             <1..1194
                     /locus_tag="ISB30_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP1992401.1"
                     /translation="VQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDC
                     GETRGRWVIEGDLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRA
                     ASEGVPQGGVISPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVREN
                     WQWKPAVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVND
                     GFIFLGHRIIRKRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNR
                     KLKGWAMFYQFVDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQS
                     KTWVLFGKTNHGKLSGEILYWLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAM
                     AFASI"
     gene            1284..1667
                     /locus_tag="ISB30_004881"
                     /pseudo
     CDS             1284..1667
                     /locus_tag="ISB30_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558190.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1696..>1946
                     /locus_tag="ISB30_004882"
     CDS             1696..>1946
                     /locus_tag="ISB30_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1992402.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVP"
BASE COUNT          505 a          469 c          549 g          423 t
ORIGIN      
        1 gttcagcggg ccatgctgat ggcgatggag ccgatatggg agagtgattt tcatacgctc
       61 tcatatggct tccggcctga gcgcagtgtc caccacgcga tccgcacggt gaaattacag
      121 ctcacagact gtggtgaaac ccggggacgc tgggtgattg aaggcgacct gtccagttac
      181 ttcgacaccg tacatcatcg actgctgatg aaggctgtac gccgcaggat cagtgacgca
      241 cgtttcatga ctctgctgtg gaaaaccatc aaggcgggac atatcgatgt cggtctcttt
      301 cgggcggcca gtgaaggtgt gccacagggc ggtgttatat cgccgctatt atcgaacatc
      361 atgctgaatg agttcgatca atacctgcat gagcgctacc tgagcgggaa agccagaaaa
      421 gatcggtggt actggaataa cagtatccaa cggggccgaa gtacggcggt cagagaaaac
      481 tggcagtgga aacccgcggt ggcgtactgc cgctatgccg atgattttgt cctcatcgtc
      541 aaaggcacca aagcacaggc ggaagccatc agggaggagt gtcggggtgt gctcgaaggc
      601 agtctgaaac tcaggctgaa catggataag actaaaatca cccatgttaa tgacggcttt
      661 atctttctgg ggcacaggat cattcgcaaa cgcagtcgtt atggcgagat gcgagtggtc
      721 tcaacgatcc cgcaggagaa agccagaaac ttcgccgcat cgctgacagc actgttatca
      781 ggcaactaca gtgaaagcaa agtcgatatg gctgaacaac tcaaccgaaa actgaaaggc
      841 tgggccatgt tctatcagtt cgttgatttt aaggccaaag tcttcagtta tatcgaccgt
      901 gtcgtgttct ggaagctggc tcactggctg gcccgcaaat accgtacagg tatcgcttcc
      961 ctgatgaggt ggtggtgtaa atcaccgaaa ccgggtcaga gcaaaacgtg ggttttattt
     1021 ggtaaaacca atcacggcaa gctcagcggc gaaatactgt actggttggt ggggcaaggc
     1081 aagaagctgt tccgctggcg gctacccgaa ggtaatccct atctgaggac ggagaccaga
     1141 aacacgtata catcgcgctt tacagaagtg gcaatggcgt tcgccagcat ttaaatggag
     1201 agccggatgc gctgaaaggt gcacgtccgg ttcggggagg agaggcaggg aaatagtccg
     1261 actacgccct gcctcttact ctactcagca catggcaggg ggtcgtctat gtggcgttca
     1321 tcattgatgt gtttgccgga tacatcgtgg ggtggcgggt ctcatcgtct atggaaacga
     1381 cattcgtgct ggatgctctg gagcaggcgt tatgggcccg tcgaccgtcc ggcacagtcc
     1441 atcacagtga taaaggttct cagtatgtat cgctggccta cacacagcgg cttaaggaag
     1501 ccggattact ggcatcaaca ggaagtactg gtgactcgta tgacaacgcg atggcggaga
     1561 gcatcaatgg cctttacaaa gcggaggtaa tacaccgtaa gagctggaaa aaccgtaagc
     1621 gtcgtctcag caccgtctgg cagatcctga aattcctgag agaatagtgg acaccaaata
     1681 tggtggacgc tatccatgaa atcattaacc gcagtgcgta aaaaaagccc taattatccc
     1741 gttgagttca aaatcaaaat ggttgaactc tcgcatcgac cagagatctc cgtagcgcaa
     1801 ctcgctcgtg agcatgggat caacgataat ttgctgttca agtggcgcca gtactggcgc
     1861 gaaggaaaac tacgtcctcc ttcaacaaca gaaaacaacg tgcctgagct gctcccgata
     1921 actcttgatg ccgaagatgt tgtccc
//
LOCUS       DACADF010000319         1937 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.324, whole genome shotgun sequence.
ACCESSION   DACADF010000319 DACADF010000000
VERSION     DACADF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.324"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..354
                     /locus_tag="ISB30_004883"
     CDS             <1..354
                     /locus_tag="ISB30_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HAP1992403.1"
                     /translation="AITKDASTLNPEGYSVVEIPDITANRRADDSGKWMFKDGAVIKR
                     VYTEEELRLQTENQKKILLQQAREKTQFWQTQLTLGIITDSDRQQLMNWMRYVQQVET
                     TDTSVLPVTFPEPPE"
     gene            complement(385..912)
                     /locus_tag="ISB30_004884"
     CDS             complement(385..912)
                     /locus_tag="ISB30_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAP1992404.1"
                     /translation="MQHLKNITAGNPKTIEQYQLTKKAGVIWLYTEDGKNWYDELKNF
                     QDDTLKIAYDQKGIIRCIEKDVSTLNPDGLSVVELPNITANRRADISGNWKFLDGKVV
                     KREYTKQELQQQAELQKAALLSEAESVIQPLERAVRLNMATDEERTRLESWERYSVMV
                     SRVDTAKPEWPQKPE"
     gene            complement(928..1896)
                     /locus_tag="ISB30_004885"
     CDS             complement(928..1896)
                     /locus_tag="ISB30_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP1992405.1"
                     /translation="MTGELKIGTVNALRIFNDAFGLIFRRSEDFLHFIPTAEGQGENG
                     DIGPLRPFAINLRTGAISVSHGAKIDGGLALGTDNALGGNSITLGDNDTGIKQGGDGV
                     LLFYSNGQLAFGLQPASADFYKRVAYIHQGIIPDGSGAFADQLNNATAPFVQTQFAWN
                     PTPGGLYVPIVKGLSIRNGQGYPGAVSFGYLLTEQYGFPVPCIHMRGDGGNDALWQFN
                     PNDKSFISPGALIAGGVRYNTDGNIFGGCWGSNLNDYLNSSFIRNVRLGGRRSDTLYR
                     GGLCEPGNGHVTTGLQIIGEVDGDDWMVSRPLQKYISGNWYNVEQA"
BASE COUNT          529 a          483 c          399 g          526 t
ORIGIN      
        1 gccataacca aagatgcctc cacacttaac cctgaaggtt atagcgtcgt tgagattcct
       61 gatattaccg ccaaccggcg tgctgacgac tcaggtaaat ggatgtttaa agatggcgca
      121 gtcattaaac gtgtatatac cgaagaagag ttgcgcctac agacggaaaa ccagaaaaaa
      181 atattattac agcaggccag agaaaaaacg cagttctggc aaacacagct tactttgggg
      241 attatcactg attcagacag acagcaactg atgaactgga tgcgatacgt gcaacaggtt
      301 gagacaactg atacctcggt tcttcctgta acattcccgg aaccaccaga ataagaaaag
      361 gcccgtacgg gccttaattt gttgttattc tggtttctgt ggccattcag gctttgcagt
      421 atccacacgg ctgaccataa cgctgtagcg ttcccatgat tccagtcgtg tgcgttcctc
      481 atcagttgcc atattcagtc tgacagcacg ttccagcggt tgaatcacag actccgcttc
      541 ggaaagcaaa gcggcttttt gtaactctgc ctgctgttgc agttcctgtt ttgtatattc
      601 ccgctttact actttaccat ccagaaactt ccagtttccc gagatatcgg cgcgacggtt
      661 ggctgttata ttcggtaact caacaacact taacccgtca gggttaagtg ttgatacgtc
      721 tttctcaata caacgaataa tccccttctg gtcataagct atttttaaag tatcatcctg
      781 aaagtttttt aattcgtcat accagttttt accgtcttct gtatatagcc agataacgcc
      841 agcttttttc gtaagctgat actgctcaat ggttttgggg tttccggcgg taatattttt
      901 cagatgttgc atatattctc ctgatggcta tgcctgttca acgttatacc agttaccaga
      961 aatgtatttt tgtagtggtc gtgacaccat ccagtcatct ccatcaacct caccaataat
     1021 ttgcaatcct gttgtcacat gaccattacc tggttcgcaa agtcctccgc gatataatgt
     1081 gtcagaacgt ctgcctccca gacgcacatt tctgataaaa gaactattca ggtaatcatt
     1141 taagtttgac ccccagcacc caccaaatat atttccatcg gtgttataac ggacgccacc
     1201 cgcaataaga gcacccggtg aaataaagga tttatcgttc gggttaaact gccataaagc
     1261 atcattaccg ccatcgccac gcatatgaat acatggaacc ggaaatccat actgttctgt
     1321 cagtaaatac ccaaagctga ccgcgccggg atagccctgt ccattgcgaa tggacaagcc
     1381 cttaactatc ggcacgtaaa gaccaccagg agtgggattc caggcaaact gcgtctgaac
     1441 aaaaggcgcg gtggcattat tcaactggtc tgcaaatgcg ccacttccat caggaattat
     1501 tccctgatga atatatgcaa cccgcttata aaaatcagca gatgcgggtt gaagcccaaa
     1561 tgccagttgt ccatttgaat agaataaaag gacaccgtcg ccgccctgtt taataccagt
     1621 gtcgttatct ccgagagtaa tggaattacc gcccagtgcg ttatctgtac caagcgccag
     1681 cccaccatca attttggccc cgtggctgac agatatagca cctgttctca gatttatagc
     1741 gaatggcctt aatgggccga tatcaccgtt ttcgccttgt ccttcagccg tcggaatgaa
     1801 atgaagaaaa tcttctgaac gacggaaaat aagaccgaag gcatcattga aaattcgcag
     1861 cgcattcacc gtgccaattt tcagttctcc ggtcatttta tcgccggaac gctgaacggc
     1921 gttaccagcc ttgttta
//
LOCUS       DACADF010000320         1902 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.325, whole genome shotgun sequence.
ACCESSION   DACADF010000320 DACADF010000000
VERSION     DACADF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.325"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(517..642)
                     /locus_tag="ISB30_004886"
     CDS             complement(517..642)
                     /locus_tag="ISB30_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP1992406.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGVTSRMLSVIPLLFFQG"
     gene            1136..1675
                     /locus_tag="ISB30_004887"
     CDS             1136..1675
                     /locus_tag="ISB30_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="firmicute plasmid replication protein RepL"
                     /protein_id="HAP1992407.1"
                     /translation="MLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLNPPDWSQVTKA
                     GWNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDL
                     ESIGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKDGVIDRKLRLMLS
                     RREEFESSLKYSNHDSEQG"
     gene            1682..>1902
                     /locus_tag="ISB30_004888"
     CDS             1682..>1902
                     /locus_tag="ISB30_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992408.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYGIEYATGINLYMNCE
                     DKEFNLVDVINEDGNKIMFVVSNDEKIQKP"
BASE COUNT          580 a          382 c          456 g          484 t
ORIGIN      
        1 aaaaaaaata gcaaagaaaa aaactccagc acctgtaatt gagctagata aatatcgaaa
       61 aaaatgacca ttgagccagc ggcaggcgga caatcagggg ctacgtgtta acgttctgac
      121 cttgattgtc tatcctgcat tgctcttttg ccgcctcgaa tcccttgcgt gtttttgttc
      181 cacgctctcc agaacgaccg agccgccacg gttccgatag cgccttgagc gcaaccggaa
      241 accgtctggg gagaacccca gacccccatc gctgtaatac cgacggtcta cgaccaccta
      301 ccagccgaat agctgcctat gccgtgcaaa taggctttgc agggggctga cgctgttcct
      361 ggcgacctga aagcccctgg ttaggggtat cggcgcgcct gacctcctga gagcctctgt
      421 aagcgttttt cgcgccttcc atgccctgac gggcatttaa aacccgtttc gtgcgtctga
      481 gagcttctga gcgcgtttga agggctatct gacgactcat ccctgaaaga acaacaacgg
      541 gatcaccgac aacatgcgag aggtgacacc gctcgccgca agtcgatgac cgagcgtagc
      601 gagcgaatcg acgaggaagc ggaagagaac cggaagccac attgagcact tacgcactga
      661 tgcggggtgt cggggtgaag ccctgaccag gtggtgaatg tctgagtgcg cgtgcgcggt
      721 ccgacattcc cacatcctgt cccgatttct gaggcttttt agcggtgaac ggacggcaaa
      781 accatgatgg agttgttgaa cgttgagagc atgaaatctg gcaaaaatca cggctggtaa
      841 ggcttgcaga ggagtaagcc agtatacact ccgctatcgc tacgtgactg ggtcagggct
      901 gcgccccgac accccaaaag cagttaggta gtgttaaatc atgagcagta aaaaaacgct
      961 ttgatagatc tttagagcaa aaattgacat ttctttaaaa atcattgact taagacacct
     1021 tcatacgaaa cgttatttgc atgtgtatac gcaaacatgt ttgcatgtgt tgtttcgtac
     1081 tgtacgatat aaatttcgta aggtgcgaaa tatcattgca ggtaaggatg caagtatgtt
     1141 ggaacagcaa tttgaagact ttgaacagca aaaacggcga gatcatcagg tacaagatca
     1201 atccaagaac gctaagcaaa aacgagatcg gttgaatcct ccagactggt cgcaggttac
     1261 taaggctggt tggaatatgt tggacgctat atcagaaaaa gttcctagag catttcggtt
     1321 atattctttt tttgcccaga atatagataa atcatgtggg gctgttgtat gtgatcaaaa
     1381 attcttagct gattatttcg gtgttagttt aagaacgatc caatattgga ttggagattt
     1441 agaaagtatt ggcgcaatag ttaaaatacc agtaggcata tattatgcct atgcattaga
     1501 tccttatcag gtatggcgtg gatataacaa tactaaaaaa tatgcagcat ttaacgcaaa
     1561 aacattaact caaaaagatg gggttataga tcgtaaattg cggcttatgc tttctaggcg
     1621 tgaagagttt gaaagttcct taaagtactc taatcatgac tcagagcagg ggtgatgcac
     1681 tatgagaatt aaatatgaat cacgttggag attagaagaa ctaaaattag aaatatgcag
     1741 ttcaatagat gagttaatta ataaatatgg tattgaatat gcaacaggaa taaatcttta
     1801 tatgaattgt gaagataaag aatttaatct tgttgatgtt atcaatgaag atggaaataa
     1861 aataatgttt gtcgttagca acgatgaaaa aatacaaaag cc
//
LOCUS       DACADF010000321         1804 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.326, whole genome shotgun sequence.
ACCESSION   DACADF010000321 DACADF010000000
VERSION     DACADF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.326"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(77..445)
                     /locus_tag="ISB30_004889"
     CDS             complement(77..445)
                     /locus_tag="ISB30_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAP1992409.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            complement(608..829)
                     /locus_tag="ISB30_004890"
     CDS             complement(608..829)
                     /locus_tag="ISB30_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAP1992410.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(892..1368)
                     /locus_tag="ISB30_004891"
     CDS             complement(892..1368)
                     /locus_tag="ISB30_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAP1992411.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEYGLL"
     gene            complement(1384..1803)
                     /locus_tag="ISB30_004892"
     CDS             complement(1384..1803)
                     /locus_tag="ISB30_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP1992412.1"
                     /translation="MQKLVATPVPDERRISFWPQHFGLIPQWVTLEPRIFGWMDRLCE
                     DYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAGIAACLMTY
                     SHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT          411 a          469 c          531 g          393 t
ORIGIN      
        1 ctgatatgca gagataaaaa atgggacagt ggcgtggtga aaacaccagg ccggagcaga
       61 aggttattct ggtggtttac tttttcgttt caggtgtcgg ataaacagcc agataaacgt
      121 aaccacagga gccgagggtg tcggcttcac aggtcagccc cttcgcatac agtgtcacgg
      181 tatgctgatg gcggggattc agttcaccac tggtgagcat taatttcagt tgtttcatca
      241 gcagcggaaa ggcctggtcc agagggtacg catccgcatc tctgaaccgg cctctgatac
      301 cggcacggtc ggcaaggtaa tgcaaccggt taccctcctg caccagacgg gcgccgaaac
      361 agggcgtcac tgtgcagggt agtccccacc agggatggtc gtcattgtca tcgggatgcg
      421 ttgtcccgga aagtgtgtct gacacgataa aatcctcaca gaaaatcggt gaatgatagt
      481 ttaacgatga cgggtcagcc ggcgcgcaga ccggcttcgg taaaggtgta ttcgttgatg
      541 agcaggacct catccacagc ggcgtctgag ctctgccagt cgtactcgtt ttccgggtga
      601 cggaacatca gttcagggtg acgctcatca gccggacata cggaccaaaa ctgtccttac
      661 ggcgttctgc aaacacggcc agcacaccgg gaatatcctg cacttcacga ccggtatacg
      721 cttcagcact gccgtgccag cggtatttgc cggtacagaa cggaaaaaga cgggatgccg
      781 gatgctgacg gtgaatacgc atggcttcgc cacgggtgat gattttcata gtgggatacc
      841 tctgaagaca gaagataaaa gtgaaaacag gtgtgatgtg gttgtgacgg gttaaagcag
      901 accatattcg gcaaaggaga aaacctggct gccaccgact atcagatggt ccggcacccg
      961 gatatccacc aggcccagtg cctgtaccag acgttccgtg ataaggcggt ctgccttgct
     1021 gggtgtgact tcaccggacg ggtgattgtg tgccagcacc acagcagcgg cattgtgata
     1081 cagggcgcgt ttaatcactt cccggggatg gacttccgtg cggttgatgg tgccggtgaa
     1141 gagggtttca ccggcaatca gctgattctg gttgttcaga tacagcaccc ggaactcttc
     1201 acgctccagt cccgccatgt tcagaatcag ccattctcgt gccgcacggg tggaggtgaa
     1261 ggccacgccg ggttcatgaa gatggcggtc cagggttttc agggcccgtt gaatgaggct
     1321 gcgctcgccg ggcgtcatct ctccgggcag aaaggaaagc tgttgcattg tacttctctc
     1381 cattcagtcg atgatgcgca taatggcgct gcattccgga tgctgcaggg cgtaatcccg
     1441 cagccggtaa taatggaccg tcatggcata acactccgta cgacaggcat gatgactgta
     1501 cgtcatcaga caggcagcaa tgccggcggc ttccgggctc atttcagcgc ggttaccgtt
     1561 catggcattg aacagtaccc atgtttcgtc atcatcgtca tccggttcgg gtgccataaa
     1621 tgccccgccg ttgttcaggg tgtacagatt ccagatacca ccgcagtagt cttcacacag
     1681 acggtccatc cagccgaaga tacggggctc cagggtcacc cactgtggaa tgaggccaaa
     1741 gtgctgcggc cagaagctga tgcgccgttc atcagggacg ggagtggcta ccagtttttg
     1801 cagg
//
LOCUS       DACADF010000322         1776 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.327, whole genome shotgun sequence.
ACCESSION   DACADF010000322 DACADF010000000
VERSION     DACADF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.327"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            272..1585
                     /gene="gltP"
                     /locus_tag="ISB30_004893"
     CDS             272..1585
                     /gene="gltP"
                     /locus_tag="ISB30_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAP1992413.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREMLGKFDKTADQ"
BASE COUNT          382 a          413 c          493 g          488 t
ORIGIN      
        1 tcactaatca gtattattgc agattaaaca aataaaaatc cttctataac aaatggttat
       61 tcattaatcc tgccataaac tcgcgttatt atgcattaat gcagcgaaaa gctctgttgt
      121 taaagggttg cgcaacatac cgcgcaaatg atactgatca taagcgttaa aaaaatctac
      181 aaaccaacgc aacacaattc atgccctggc agtatgtcac gttctcgcgt ttctgaacgg
      241 ggaacggcgc tccattgagg aagttattct gatgaaaaat ataaaattca gcctggcctg
      301 gcagattctg tttgctatgg tgctgggcat tctcctggga agctacctgc actaccatag
      361 cgacagccgc gactggctgg tcgtcaattt gctctctccg gcgggtgata tcttcatcca
      421 tctgatcaaa atgattgttg tgccgattgt gatctccacg ctggtggtgg gtatcgcggg
      481 tgttggtgat gccaaacagc tcgggcgtat tggtgcgaaa accattatct acttcgaggt
      541 gatcaccacc gtcgccatca ttttggggat cactctggcg aacgtcttcc agcccggtgc
      601 cggggtggat atgtcgcagc tggcgaccgt cgatatctcg aaatatcaga gcactacgga
      661 agcggtacaa agcagttccc acggcattat gggcacgatt ttgtcgctgg tgccgacgaa
      721 catcgtggcg tcgatggcga aaggcgaaat gctgccgatc atcttcttct cggtgctgtt
      781 tggtctgggg ctttcatccc tgcccgcgac gcatcgtgaa ccgctggtga ccgttttccg
      841 ctccatctct gaaaccatgt ttaaagtgac tcacatggtg atgcgttatg cgccggtggg
      901 tgtgtttgcg ctgattgcgg tgacggtggc taactttggt ttctcttctc tgtggccact
      961 ggcgaaactg gtgctgctgg tgcatttcgc cattctgttc ttcgcgctgg tcgtactggg
     1021 aattgtggcg cgcctgtgcg ggttaagtgt ctggatcctg attcgtattc tgaaagatga
     1081 gctgattctg gcgtactcta ctgccagttc tgaaagcgtg ctgccgcgaa ttattgagaa
     1141 gatggaagcc tacggtgcac cggcgtcgat caccagtttc gtggtgccga ccggttactc
     1201 ttttaacctt gatggttcga cgctgtatca aagtattgcc gctattttca tcgctcagct
     1261 gtatggcatt gacctgtcca tctggcagga aatcattctg gtgctgacgc tgatggtgac
     1321 ctcgaaaggg attgctggcg tgccgggcgt gtcgtttgtg gtgctgctgg caacgctggg
     1381 tagcgtaggt attccgctgg aaggtctggc gtttattgcc ggtgttgacc gtatcctcga
     1441 tatggcgcgt actgcgctga acgtggtggg taatgcgctg gcggtgctgg tgattgccaa
     1501 gtgggaacac aaatttgacc gtaaaaaagc gctggcctac gagcgtgaaa tgttgggcaa
     1561 atttgataaa acagcagatc agtaattgaa gattgccggg gggatatcca cccggcaatg
     1621 tgtggatgcc tgactgagta atagcctgat gtagcgtgat ttttgtaggt cggataaggc
     1681 gtttacgccg catccgacat caacgcctga tgcgacgctt aacgcgtctt atcaggccta
     1741 cgccagacag cgcaatagcc tgatttggcg tgattt
//
LOCUS       DACADF010000323         1753 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.328, whole genome shotgun sequence.
ACCESSION   DACADF010000323 DACADF010000000
VERSION     DACADF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.328"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(115..1653)
                     /gene="cadC"
                     /locus_tag="ISB30_004894"
     CDS             complement(115..1653)
                     /gene="cadC"
                     /locus_tag="ISB30_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator CadC"
                     /protein_id="HAP1992414.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT          520 a          354 c          365 g          514 t
ORIGIN      
        1 atttatgtaa taaaaattat gcggcaaata aattgccgca acatattata ccaacgggaa
       61 catacaaaaa ctcaacaaca aatattcccg agcatgaatc aacccgggag ttacttattc
      121 tgaagcgaga aatttgtcga gataaggtac aacataagga acagaagtct ggaatatacc
      181 attttcaatc cagtaaaggg tgtttgcccc tgggcgtaaa ttaaaggcgg tgagatatgc
      241 atcagctgct tcccggttca tccccttcat ttcataaacc ttgccaagca acacataatt
      301 tagccaggac atttcaagat caatgccggt atttatcgcc tgataagact catctgtttt
      361 accttttacc agggcactga ccgcttttat ttgatatata atggacaggt tgttcaattc
      421 cggcagtgta acaatgttat ctatttctgt gttcagtgct gctaattgtt tttcgtctaa
      481 aggatgttga gaatggcgca cgatatcaac taatgctttt tctgctctcg cgtaggtaaa
      541 ttctggggat gattgaacaa tctcacctaa taattcactg gcacgatcca atgatttatc
      601 atcaccatgc agtaaataat catgtgcctg ataaaaatta gttaataacg caccacgatg
      661 cggcaaaatt tgctggagca tctcctgcat tcgttgtggc cacggttggt ttaacgcttt
      721 tgataaactc tggagcaaat cattttgaat cgccagctga ttaccgttag tgatgacata
      781 acgtttatcc agcatggttg aaccatctgc attgtctacc aattttaccg acataaagca
      841 ttgttgagca cggtattggc gctgattaac aaacgcaata gataatgttt taccggaact
      901 gctcggttca tcaatgttgt agttgatttt gtcatgcacc ataaaggtgg agaaggtgtt
      961 aagtgatgtc gccaccaaat cacccacgcc tatcgcgtaa gagagctgat acggagaact
     1021 ccagctgtta caactcttat taaccatatt aatgtcaata tcgcgtggat tgagcaaaat
     1081 gcgcgattta ctcataggaa gacgtgtttc aagacttgaa aacgctacca gtgcgacaca
     1141 gatacctaac gacaacagga aaaaaaacca tacccaaaag gtagtgaatc gtttgctttt
     1201 aactggggat tgttcaggtg gcgttgtggt gttttgaatg ttaagactgt gggagggaga
     1261 atctgtggca ggaaccgcct ctggtatagg gggaggcgaa gatagcatta tttcctctcc
     1321 ctcttcttcg ctgtaccaga taaccggcac cattaattta tagccgcgct ttggtacagt
     1381 agcgatatag acaggactat cttcatcatt atcttttaat gacttacgta gttctgagat
     1441 actctgcgtc acaacgtgat tggtgacaat acttctcttc cagacattat cgataagttc
     1501 atctctgcta agtacttcgc cactgtgttg agcaaagaaa accagaagat cgattaatct
     1561 cggctcaagg gtaagttgac gcccattgcg gctaatttgg tttatggacg gagtaacaag
     1621 ccattcgcca acgcgaacta caggttgttg cataatagaa actcattcga aaagggaatg
     1681 atgcaatgat aattgccata acctattttt tccatctata gatgggttta tttacatatt
     1741 attggtgaat gca
//
LOCUS       DACADF010000324         1743 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.329, whole genome shotgun sequence.
ACCESSION   DACADF010000324 DACADF010000000
VERSION     DACADF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.329"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            181..1386
                     /gene="chrA"
                     /locus_tag="ISB30_004895"
     CDS             181..1386
                     /gene="chrA"
                     /locus_tag="ISB30_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HAP1992415.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            1397..1702
                     /locus_tag="ISB30_004896"
     CDS             1397..1702
                     /locus_tag="ISB30_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1992416.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
BASE COUNT          326 a          459 c          512 g          446 t
ORIGIN      
        1 gatcgcctca gaaaacggaa aataaagcac gctaagccgt aagtaagcgt gctcctgtga
       61 aagccacagc taaaactgcg tagtacacat agaggtattt ttttctagat ttatatcgaa
      121 tttcgttatc gaatttcgct tattattgat caactcgttg cagatcatga aggcgaaagt
      181 atgacgaata accctaccga tgacagcaga ccatggtcgg tctttcttat ttttctgcgg
      241 cttggattga catcttttgg cggccccatt gcgcacttgg gctacttccg cgccgaattt
      301 gtcacacggc ggcgctggct ctccgaacgg agctatgctg acttggtcgc gctttgtcag
      361 ttcttgccag ggcctgcaag cagccaggtc ggcatagcgg taggactgtc tcgggctgga
      421 tacagcgggg cgctggctgc ttgggctggc ttcacgctgc cgtctgccat agccttgatc
      481 ctttttgcgc tcggcatctc cagctatggc gattacgtct cgcagggcgc gttgcatggc
      541 ttaaaagtgg tggctgtggc cgtggtcgct caagcagtat ggggcatggc gcgtaaccta
      601 tgcacggatg ggctgcgagt caccatcatg gcaattgcta cctgcgtcgt tttacttgtg
      661 ccgtccgcgt ggggacaggt tggcgtgatt gctatcgcag gcatcgcagg ccggttattg
      721 ttcaagccag cgaaagttgt tgagcatgac cccctaccta tcacggtcag tcaccgggcc
      781 ggcgtgcttt ggctctcgct gttctttgtc ttgctgattg gcctgccggt gttggccgaa
      841 ctgatgccaa gtcaaaccat ggcaatggtg gattccttct atcgtgtcgg atcactggtg
      901 ttcggcggtg gtcacgttgt gctgccatta ctgcaagccg aagtggtgcc ctccggctgg
      961 gtcaacaatg aatcctttct cgcggggtac ggggcagctc aagcggtgcc cggccctttg
     1021 ttcacgttcg ccgcgtttct tggtgcctcg atgaacaccg ccccgtcggg ctggatcggc
     1081 ggcattgtgt gtctgctggc tatcttcgcg ccctcgttct tgctggtcgt cggatcaatg
     1141 ccattttggg agcgtttgcg ccgcaataca ggcatccaag ctgcgctggc cgggatcaat
     1201 gccgctgtag tcggcttgct gctggccgcg ctgtatcagc ctgtatggac tagcgccatc
     1261 tttcagccgc aagacttcgg cttggcatta gttgcccttg tcgcacttat gttctggaag
     1321 ctcccgccgt ggctggttgt cctcggtagc ggtgcagctg ggtggctgtt gagcgtcgct
     1381 ctgtgaggca caaaaaatga ctgacaaaga cctctacggc ggtttgatcc gcctgcacat
     1441 ccttcaccat gcagccgagg aacctgtctt tgggctgggg atcatcgaag agctacgccg
     1501 acacggctac gagatgagcg ctggcaccgt gtacccgatg ctgcacggcc tggaaaaaaa
     1561 aggctatctg acctcacgcc acgaacgcac cgggcgacgt gaacggcgtg tttatgacat
     1621 cactgaacaa ggcagaactg cacttgctga tgcgaaaaca aaggtcaagg agctgttcgg
     1681 agagttggta gaaggtggtt gagtttttgc ttctatgtca ggttgagcta taccctaact
     1741 gga
//
LOCUS       DACADF010000325         1738 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.330, whole genome shotgun sequence.
ACCESSION   DACADF010000325 DACADF010000000
VERSION     DACADF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.330"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(33..794)
                     /gene="kduD"
                     /locus_tag="ISB30_004897"
     CDS             complement(33..794)
                     /gene="kduD"
                     /locus_tag="ISB30_004897"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAP1992417.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(824..1660)
                     /gene="kduI"
                     /locus_tag="ISB30_004898"
     CDS             complement(824..1660)
                     /gene="kduI"
                     /locus_tag="ISB30_004898"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP1992418.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
BASE COUNT          447 a          472 c          396 g          423 t
ORIGIN      
        1 tgcgacaggg tcataaaagt aagaggaatg aattaacgcg ccagccaacc gccatccacg
       61 gcaatggtat aaccattcac ataatctgaa gcgctggagg caaggaacac caccggcccc
      121 atcaggtcac tcggcagtcc ccaacggcca gctggaatgc ggtcgagaat ttccgcgcta
      181 cgttgttcat ctgcccgcag ttgttgagta ttgttggtcg ccatgtaacc cggagctatc
      241 gcattaacat taatgttgtg tttagcccat tcgttcgcca tcaatcgcgt cacgcccatc
      301 acaccgcttt ttgatgcggt ataagaaggc acacggatcc cgccctggaa ggagagcatt
      361 gacgcgatat tgataatctt gccgccattg ccttgcgcga taaagtgttt cgccgctgcc
      421 tgagacatga agaatacgct cttgatattc aggttcatga catcgtccca gtctttttcg
      481 ctgaattcga gagcgtcttc gcggcgaatc aatccggcgt tattcaccag gatatcaata
      541 tgaccaaact ccgctaccgc gcgatccagc agtgctggaa tgccatcaat ctttcgcaga
      601 tcggcggtca ggcttaaaaa acgacgcccc agcgccgtga cctgcttgat ggtttcagtc
      661 ggttcaacga tgttaatgcc aacaatgtca cagcccgctt gcgccagccc caacgccatc
      721 ccctggccca gcccagtatc acaaccagtg acgaccgcaa ctttaccttc gagagaaaat
      781 gcacttaaaa tcataacaat accttattcg tttatgccca caactagcgc aaatctttaa
      841 cggccacatg gtccatatca tcaaaaacct ggttttcacc gaccatgccc cagataaagg
      901 tataagcttt ggttccgacg ccggaatgga tcgaccagct cggggagatc accgcctgct
      961 cgttatgcat cacaatgtga cgcgtttctt gcggttgccc catcatgtgg aaaacgcagg
     1021 cgtcatcatc catattgaaa tagaaataaa cttccatccg gcgctcgtgg gtgtgacacg
     1081 gcatggtgtt ccacaagtta cctggagcca gctccgtcag ccccatactc aattggcagg
     1141 tttccagcac atccgggaca aaatatttgt taatcgtgcg acggttactg gtgaggttat
     1201 cgcctaacgt gactggagat acttcgtccg gtgtgacttt tttggtggga tacgtcgtat
     1261 gcgcgggtgc gcaattgtaa taaaacttcg ccggagtggc ggtatcaata ctggcaaaga
     1321 caacttcttt tgcaccttta ccaacataca gggcgtcgcg gtgaccgatt tcatagcatt
     1381 gaccatcgac agtaatcgta ccggcaccgc caatattgat aacacctaac tcgcgacgtt
     1441 caaggaaata gcttacgccg agttgtttac caacttcccc gccaacggaa accgttttag
     1501 ttaccggcat aatgccgcca acaataatac ggtcaatgtg gctgtaaacc atggtgtact
     1561 catcggcgac aaataccttt tcaaccaaaa attcattgcg cagcccctgg gtatccagcg
     1621 tttttgcgtg cgcgctgtgg atgctctgtc ttacgtccac atcaacctcc gataatcagt
     1681 gaagcgtgaa aatagaaaac aataataaaa cactgtttcg ttttctctat gaggggat
//
LOCUS       DACADF010000326         1736 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.331, whole genome shotgun sequence.
ACCESSION   DACADF010000326 DACADF010000000
VERSION     DACADF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.331"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..745)
                     /locus_tag="ISB30_004899"
     CDS             complement(<1..745)
                     /locus_tag="ISB30_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAP1992419.1"
                     /translation="MRLTSRFGYVNQIRRDRPLTHEELMHHVPGIFGEDRHTSCSERY
                     TYIPTITVLESLQREGFHPFFACQTRVRDPDRREYTKHMLRLRRNGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHRPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIH"
     gene            complement(966..1376)
                     /locus_tag="ISB30_004900"
     CDS             complement(966..1376)
                     /locus_tag="ISB30_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992420.1"
                     /translation="MKTFIKTLLVAVTILFSVFAAAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNPFNYKDNSYVFVLERGGAWCYDYTVSVLNLKTGKEQMLEYKDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVALDKLKT"
     gene            complement(1392..>1736)
                     /locus_tag="ISB30_004901"
     CDS             complement(1392..>1736)
                     /locus_tag="ISB30_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992421.1"
                     /translation="YELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVT
                     INGTVWLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEF
                     AYLVKNYVAAVN"
BASE COUNT          401 a          438 c          439 g          458 t
ORIGIN      
        1 tgtgaatggc acgggtgtgg atacgttttc cttttgcact gcgaccggaa atcccgcctt
       61 tcagcatatt ctcctggatg gtctgataag tactccacag gtccttaccg taatcctccc
      121 ggcgtcgtgg cgtcagaatg tcggcggtgg tgacgggccg atgttcgtca ccataacggt
      181 aagtcagtgc cgcctgtgcc agcgcctggc gtgccggtgg cggcagaatc agcgactgca
      241 tggcatcacg cttctcctct atccggtcaa acacgcccac cacctcgtaa gccccttcaa
      301 tgactctgtc caccacattt ccccggtgcg gaacacgcac ttcccccaga gactgaccac
      361 agacgcatcc gttctggcag acgaacctga agtaacccgg cagcatctgg tagctggagg
      421 taccgtcatg agagttgagc agaataattt cagggacatg ttctccgttt atctctccgt
      481 tacggcgcag acgcagcatg tgtttggtgt attcccggcg gtccgggtca cgtacgcggg
      541 tctggcaggc gaagaatgga tgaaagcctt cccgctgcag gctttccagt acggtgatgg
      601 tggggatgta cgtatagcgt tcactgcagg aggtatgccg gtcttcaccg aaaatacccg
      661 gtacatggtg catcagttct tcgtgtgtca gcggacggtc acggcgtatc tggtttacat
      721 aaccaaaacg actggttaat cgcatgattt gctccttatc ggtggttaag atttactggt
      781 gtaataaatg aaaaaagcca cgtctcccgg agaagacgcg gcctgacggg tgaaatgaat
      841 gaggtttatt gtctgagaag cctttcactg gcgagccacg cattaagcgg ctttccggca
      901 acaatggctg gggctgtatt tacctttgat attaataatg tgcagagaaa cgcatggttt
      961 actcctcagg ttttaagttt gtccagagcc acaacgacag gttttccgga tgtgtcgatg
     1021 actcctaccg tcggtacgcc atttttgatt tcgaagaacg gtttgttgct acctgagcac
     1081 agctggttgt ctttgtattc gagcatctgt tctttcccgg ttttcaggtt aagtaccgag
     1141 acagtgtagt cgtaacacca ggcaccacca cgttcaagca cgaaaacata gctgttatct
     1201 ttatagttga acggattctt cgtctggcaa ttcataccgt caatctcagt acaggaaaac
     1261 gcctctgtag tattaacgta tttgatgttg tttggcagtt ttacttgttt cgccgcagcg
     1321 aagacagaga acagaatagt tacagcaacg agtaaagttt tgataaaagt tttcatgaca
     1381 gactccttaa attagttaac cgcagctacg tagtttttca ccagatacgc gaattcatgc
     1441 acgccaattt cgccgaacca aaccaggtag gccgggtcat ctaaaccaaa gtagcggtag
     1501 ccaacgactt catgggtttt tgggttatag agtgttacga taaagtccca gccgcaggta
     1561 ttggctttgc aaccgtcgcc ctggagccag actgtaccgt taatggttac tggcgttacg
     1621 tttccgccag ggccatccat atacccgcct ttaccgaaca tgccgctcag tccgaccggt
     1681 tttaatgctt tgtcaagcgc agctttgtag gcagcgtcac cttcgtacaa ctcgta
//
LOCUS       DACADF010000327         1697 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.332, whole genome shotgun sequence.
ACCESSION   DACADF010000327 DACADF010000000
VERSION     DACADF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.332"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..241)
                     /locus_tag="ISB30_004902"
     CDS             complement(<1..241)
                     /locus_tag="ISB30_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HAP1992422.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSGFFTDTPGKMGL"
     gene            370..717
                     /locus_tag="ISB30_004903"
     CDS             370..717
                     /locus_tag="ISB30_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992423.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(737..1246)
                     /locus_tag="ISB30_004904"
     CDS             complement(737..1246)
                     /locus_tag="ISB30_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992424.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLPAGGTSATDLAV
                     ELNGITYQACRGDFVVRLDGSTCLQLWNKEGRVVRREGDPLEVAQWLQACHDAGMEVR
                     VQINESAAP"
     gene            complement(1243..1503)
                     /locus_tag="ISB30_004905"
     CDS             complement(1243..1503)
                     /locus_tag="ISB30_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992425.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          384 a          468 c          426 g          419 t
ORIGIN      
        1 gcaatcccat ttttccaggt gtatcagtaa aaaaaccgct gttccagaaa tcaggccggg
       61 taaatttcag agctgtatct tcaatatacc attttgcaac cggatacaaa accattccgc
      121 agagagaaat acaccagaac agtaatctgt atttaaaatc ataatcccat gacatataca
      181 gcatatatcc ccccgtcacc catccccacc acatattatt aaaataatat tttctgttca
      241 tctgccagtt ttcctttctt ttgcactttt gtcagtgtac tgatgcatga caacataacc
      301 acagcatgat aataataatc aataacaata agctgtgtca cgtttacatc atggaacgga
      361 ggtgctcctg tgacttttga gcagaaaaaa gcccgggcca ttgccctgat ggacagcaaa
      421 aagatgtggc ggagcaatta tgcccctcct cttctgcgta ttctctggcg gctgggtatc
      481 cggcttccgc cattaccctt tatgccgttc tggcaggtca cggtgctgac ggggggctta
      541 tggggcattt cctggggatg tgcaatgtgg tttatctact gggggccgtc aggaatggtg
      601 gcaggtgagg ccattattat aagtatcacg ggggggtttt tgtccggact gctcatggcc
      661 tctttccact ggtggcgcag gaaagttaac cggctgccac catgggatga tgtgtaaacc
      721 gtgcacaggc gtgttcttac ggagcggcac tttcatttat ctgtacgcga acctccattc
      781 ctgcatcgtg acaggcctgc agccactgcg ccacttccag cggatcgccc tcccggcgta
      841 ccaccctgcc ttctttattc cataactgca gacaggtgct gccgtcgaga cgcaccacaa
      901 aatccccacg gcaggcctga tacgttatgc cgttcagctc caccgccaga tcagtggcag
      961 aagtgccgcc tgcgggcagc agaggctgtg ccacggcccg gccggcgtcc gtcagctcca
     1021 ctgccagctg caggttcgga gcgcgcaggg tacgcagcca gccggcatcc tccagacgac
     1081 ggcaggaggc gcgcaggttt ggcccgtaga ccggggcttc cccgctttgc tccagcaccc
     1141 gttctatgtc ccgcgtcagc acgggtcctg gccgtttttc atcaagggca gccagcacaa
     1201 tcagcactct ccgctgcagt ggcgacggtc ggcggctctg agtcatttcc agtcatcctc
     1261 cggccagatg agctttctga ggcgctgatg gtcggcgtca atgatctcct gtctctttat
     1321 ggcattcatt gaggtatagc catttatggc cagttcatgc cagaagatca gcacggctcc
     1381 ggctttggct gactcgcaga gataagttac gccgtggctg tccagattgc attcccggat
     1441 gcgtttttcc gcaaaccggg tcagttgttc atagctgagt gaaaacgtca tttcctgatc
     1501 catcctgtac gttccatctg ataatgttct gacaggtaaa aatcatatcc tgaaccgccc
     1561 cgggaatcct ggagactaaa ctccctgaga aagaggtaaa caggatgact aaaaatactc
     1621 gtttttcccc cgaagtccgt cagagggcga ttcgtatggt tctggaaagt cagggcgaat
     1681 atgactcaca gtgggcg
//
LOCUS       DACADF010000328         1685 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.333, whole genome shotgun sequence.
ACCESSION   DACADF010000328 DACADF010000000
VERSION     DACADF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.333"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..1667
                     /locus_tag="ISB30_004906"
     rRNA            <1..1667
                     /locus_tag="ISB30_004906"
                     /product="23S ribosomal RNA"
BASE COUNT          425 a          373 c          532 g          355 t
ORIGIN      
        1 gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg
       61 ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg
      121 tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga
      181 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
      241 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcgaca
      301 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      361 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      421 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagcgacttg
      481 ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      541 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      601 taattgatgg ggttagcggt aacgcgaagc tcttgatcga agccccggta aacggcggcc
      661 gtaactataa cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga
      721 atggcgtaat gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg
      781 aagatgcagt gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac
      841 actgaacatt gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt
      901 ctgcatggag ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtgggcccc
      961 ttaccggggt tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag
     1021 agtaacggag gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat
     1081 ggcataagcc agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag
     1141 tgatccggtg gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat
     1201 aacaggctga taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg
     1261 ctcatcacat cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg
     1321 gtacgcgagc tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct
     1381 ggagaactga ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt
     1441 tcgggttgtc atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa
     1501 agcatctaag cacgaaactt gccccgagat gagttctccc tgactccttg agagtcctga
     1561 aggaacgttg aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag
     1621 ctaaccggta ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga
     1681 agaga
//
LOCUS       DACADF010000329         1684 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.334, whole genome shotgun sequence.
ACCESSION   DACADF010000329 DACADF010000000
VERSION     DACADF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.334"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..1376
                     /locus_tag="ISB30_004907"
     CDS             <1..1376
                     /locus_tag="ISB30_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP1992426.1"
                     /translation="VNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGFMAMHLGTNFYL
                     KQHDLLVVADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKIVNLRIPESLGF
                     YSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVGYFFYAVAERVT
                     IPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIPLDLAGKMEQGK
                     LMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIEFLFLFVSPLLF
                     VFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAVCVEVAYFFIYY
                     FVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADIPTPDKSELQAAEQMVKALGGKE
                     NIKSLDNCVTRLRLTIADMGLIDEVAIKIAGGIAVVKLDQNTLQVIIGTKVIALRRDM
                     DNYMGIY"
     gene            1376..>1684
                     /locus_tag="ISB30_004908"
     CDS             1376..>1684
                     /locus_tag="ISB30_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-cystathionase"
                     /protein_id="HAP1992427.1"
                     /translation="MDVFNTPVSRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYYQQYQTDIKPEWITYSPSVMYSIAK
                     A"
BASE COUNT          427 a          334 c          406 g          517 t
ORIGIN      
        1 ttgttaattt acctgtattg tttgcgatag cgattccctt aggattatta aaagataaag
       61 aggataaagc ctatggtgct ttttctggct taattggttt tatggcgatg catctgggaa
      121 cgaactttta tcttaaacag cacgacttat tggtcgttgc tgaccaaatg tcgacacatg
      181 ggcaaaccat cattctgggg atccagtcct acaataccag cgtgttgggg ggaattgttg
      241 ctgggttatt agtcgccagc atgtataaaa agatcgttaa tttacgcatt cctgaatcgt
      301 taggttttta tagcggccca cgtctggtgc ctatcattac actgattgtg atgagtggat
      361 ttggtctgat cattcctttt atctggccgc cgtttttcaa tcttttcatg ctcattggac
      421 actggatttc aacttccggt cctgttggtt atttcttcta tgcagttgcc gaacgcgtga
      481 cgattccttt tggcttaaac catctggtga cgtcagtttt ccgctttacg ccaatcggcg
      541 gttcggctgt gattggcggc gaagaatatt acggcaccct gaacatgttt atggcgtacg
      601 tcaaagagaa tgcggtcatt ccgctggatt tggcggggaa aatggagcag ggcaaactga
      661 tgattcagta tggtctggct ggtgccgcgc tggcgatgta tcgcactgct catgctcaaa
      721 acagaaaggc tatcaaagca ttgcttattt ccggggtgct tacggtgatt attggcggcg
      781 tcagcgaacc gattgagttt ctgttcttat ttgtcagtcc actgctgttt gtcttccatg
      841 cctttatgaa tggattcgct aacatggttc tgccatatat gggcgtgaag atgggattta
      901 ctggcgatct gattcaattt attagctttg gcgtattgcg tggcacaaga acaggttggc
      961 cgatcgcggt gtgtgtcgaa gtggcctatt tcttcattta ttactttgtg ttccgttgga
     1021 ccattcttaa atttaacctg atgaccgtag gccgtgaaga gtccagtcct gtcacgctga
     1081 acgctcacga agatacggct atagcggata tcccaactcc tgataaatca gagctgcaag
     1141 cggcggagca gatggttaag gcacttggtg gtaaagagaa tattaagtca ctggataatt
     1201 gcgtaactcg tttacgttta acaatcgcag atatgggatt gattgacgaa gttgcaataa
     1261 aaatagctgg cgggattgcg gttgttaaac ttgatcaaaa taccctacaa gtcattatcg
     1321 gcactaaagt catcgccctg cgtcgggata tggataacta tatggggata tactgatgga
     1381 tgtttttaat actcctgttt cccgcaaagg aacatattgt acccaatggg acttttgcga
     1441 ggacagattt ggtgtaaaag atgtactacc attctctatt tcagatatgg atcttccgat
     1501 accagacgct atcaccagag cattaaaaaa acgcctggaa cacccgattt tagggtacag
     1561 tcgttggcaa catggtgaat atctgaatgc gatagtaaac tggtattatc aacaatatca
     1621 aacagatatt aaacctgaat ggataaccta tagccccagt gtgatgtact ctattgcaaa
     1681 agca
//
LOCUS       DACADF010000330         1610 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.335, whole genome shotgun sequence.
ACCESSION   DACADF010000330 DACADF010000000
VERSION     DACADF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.335"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            22..1164
                     /locus_tag="ISB30_004909"
     CDS             22..1164
                     /locus_tag="ISB30_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP1992428.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNYDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTALNPQRVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT          478 a          350 c          350 g          432 t
ORIGIN      
        1 ataaacattt gaggtattgt tatgacaaat aaacacccat cattgttttc tccttttatg
       61 ctgacagaaa aaataaagct acgtaatcgt atcgttatgg ctcctatgac aacctggtca
      121 gctaatcctg atggaaccat ctctgagcaa gaactcgaat tttataaacg tcgctcacaa
      181 aatgttggtt tagtaataac aggatgtacc tatgtcacgc caagcggcat tgggtttact
      241 catgaatttg ccgcgtatga cgaccgtttc attaacagcc ttgaaaagtt agcagctgca
      301 gccaaaagtg gcggtgctcc ggctattttg cagattttcc acgccggtaa taaagccatc
      361 ccggagcttg tgccaaatta cgatgttatt agtgccagcg catcaagcgt aaaatctggt
      421 gactttatga agagagtggt acaaagcagg gaaatgactg aaaacgaaat tcaggaaacc
      481 attagagcat tcggagatgt cacaaaaagg gcgattaaag caggttttga tggcgttgaa
      541 cttcatggtg cgcacggatt ccttctgcag aatttcttct cccctctatt taaccagcgt
      601 aatgaccgat ggggaggaga tcttgaagga cgtatgcgct tcccgctggc cgtattacag
      661 gaagtaaaaa atgtagtcta tgaatatgcg acaaagccct ttgctatcgg ttacagaata
      721 tctcctgaag aatctgtaac agggggactg cggatagagg atacttataa actccttgat
      781 cggctcattt catccggtat ttcgtatata catacctcgc tggtcagtat taacgatagc
      841 taccctgttg agtcgccaaa tggtccccga actattgaat tgattttaaa tcatattgca
      901 ggcagagtgc cggtaatcgc cgcaggtaaa atacgcacac cgtctcaggc tcaagaagca
      961 atttccgctg gcctgccact tgttgcgatc ggtaaagggc tggtgataaa tccagaatgg
     1021 gtgactttag ctgaatcagg tagaagtcat gaaattcaaa cagcccttaa tccacaacgc
     1081 gttcctgaat taaccattcc ggataaacta tgggatcaaa ttcaggcatc gaaaggaact
     1141 ggctggtttc cgttaatgga ttaaatatcg attactctcc cgagagtaaa actatcaaat
     1201 caatgagtaa ccgatacagc ctcgcaaatt ctttcagata attaacccgt cactgcggca
     1261 cgttctctcg caaggcgtgc ctgcagtgta atccagtatg tatccggcat agttgacggt
     1321 ttaatagact caattcctct ccctggttac ctacaacgat atcttgtata acaccccagg
     1381 agatacgacg ttacgatgag atgcgatatg ctcctcagcg ccaatcaacg ccaggactgg
     1441 gccagtcttg agatcagact cattcccgta actgatttca ggcgacatat atcaatgacg
     1501 ttggggcaat aaaagatagc cgatgtaata catctattca aatttgtgat acatgtcaaa
     1561 aaattggttg accaaactcg ttatttatat aagggcactt acgaagtgca
//
LOCUS       DACADF010000331         1555 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.338, whole genome shotgun sequence.
ACCESSION   DACADF010000331 DACADF010000000
VERSION     DACADF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.338"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(285..1124)
                     /gene="sul1"
                     /locus_tag="ISB30_004910"
     CDS             complement(285..1124)
                     /gene="sul1"
                     /locus_tag="ISB30_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HAP1992429.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(1118..1465)
                     /locus_tag="ISB30_004911"
     CDS             complement(1118..1465)
                     /locus_tag="ISB30_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HAP1992430.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
BASE COUNT          354 a          459 c          450 g          292 t
ORIGIN      
        1 cgaaggtctc cgcgaatgtc cggtcgatcc gcgcgacgtc ccaggcgggc gttcccttgg
       61 cggacatcca cgccgcagcg tcgtgcatca gccgcacaac ctcgtcgata tcacccgagc
      121 aggcgacccg aacgttcgga ggctcctcgc tgtccattcg ctcccctggc gcggtatgaa
      181 ccgccgcctc atagtgcagt ttgatcctga cgagcccagc atgtctgcgc ccaccttcgc
      241 ggaacctgac cagggtccgc tagcgggcgg ccggaaggtg aatgctaggc atgatctaac
      301 cctcggtctc tggcgtcgcg actgcgaaat ttcgcgaggg tttccgagaa ggtgattgcg
      361 cttcgcagat ctccaggcgc gtgggtgcgg acgtagtcag cgccattgcc gatcgcgtga
      421 agttccgccg caaggctcgc tggacccaga tcctttacag gaaggccaac ggtggcgccc
      481 aagaaggatt tccgcgacac cgagaccaat agcggaagcc ccaacgccga cttcagcttt
      541 tgaaggttcg acagcacgtg cagcgatgtt tccggtgcgg ggctcaagaa aaatcccatc
      601 cccggatcga ggatgagccg gtcggcagcg accccgctcc gtcgcaaggc ggaaacccgc
      661 gcctcgaaga accgcacaat ctcgtcgagc gcgtcttcgg gtcgaaggtg accggtgcgg
      721 gtggcgatgc catcccgctg cgctgagtgc ataaccacca gcctgcagtc cgcctcagca
      781 atatcgggat agagcgcagg gtcaggaaat ccttggatat cgttcaggta gcccacgccg
      841 cgcttgagcg catagcgctg ggtttccggt tggaagctgt cgattgaaac acggtgcatc
      901 tgatcggaca gggcgtctaa gagcggcgca atacgtctga tctcatcggc cggcgataca
      961 ggcctcgcgt ccggatggct ggcggccggt ccgacatcca cgacgtctga tccgactcgc
     1021 agcatttcga tcgccgcggt gacagcgccg gcggggtcta gccgccggct ctcatcgaag
     1081 aaggagtcct cggtgagatt cagaatgccg aacaccgtca ccatggcgtc ggcctccgca
     1141 gcgacttcca cgatggggat cgggcgagca aaaaggcagc aattatgagc cccataccta
     1201 caaagcccca cgcatcaagc ttttgcccat gaagcaacca ggcaatggct gtaattatga
     1261 cgacgccgag tcccgaccag actgcataag caacaccgac agggatggat ttcagaacca
     1321 gagaaagaaa ataaaatgcg atgccataac cgattatgac aacggcggaa ggggcaagct
     1381 tagtaaagcc ctcgctagat tttaatgcgg atgttgcgat tacttcgcca actattgcga
     1441 taacaagaaa aagccagcct ttcatgatat atctcccaat ttgtgtaggg cttattatgc
     1501 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aatta
//
LOCUS       DACADF010000332         1547 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.339, whole genome shotgun sequence.
ACCESSION   DACADF010000332 DACADF010000000
VERSION     DACADF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.339"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            127..411
                     /locus_tag="ISB30_004912"
     CDS             127..411
                     /locus_tag="ISB30_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAP1992431.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            415..750
                     /locus_tag="ISB30_004913"
     CDS             415..750
                     /locus_tag="ISB30_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAP1992432.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(809..1408)
                     /locus_tag="ISB30_004914"
                     /pseudo
     CDS             complement(809..1408)
                     /locus_tag="ISB30_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          399 a          342 c          392 g          414 t
ORIGIN      
        1 ttaagcagat cttaattgaa gacgaaatta ttgtgtgtgc aacttactta ataccggaca
       61 actaagttga agaaaaaaat actcgccttc gggcttatct cagcattgtt ctgctccacg
      121 ccagcaatgg cggatatgaa tcgcaccacg aaaggtgcat tgctaggtgc aggcgttggt
      181 ttacttactg gcaatggtgt taatggcgta ctgaaaggtg cagctgttgg cgctggtgtt
      241 ggtgcagtaa cagaaaaagg ccgtgacggt aaaaatgcgc gtaaaggtgc aaaagtgggt
      301 gccgccgttg gtgctgtcac tggcgttctg accggcaatg gcctcgaagg ggcgattaaa
      361 ggtgccgtga ttggcggtac gggtggtgca attctgggta aaatgaaata aggaatgatg
      421 atgaaaaaat ttgctttact ggcgggtctg tttgttttcg caccaatgac ctgggcgcag
      481 gactacaata ttaagaacgg tttgccatca gaaacctata ttacttgcgc agaagccaac
      541 gaaatggcaa aaactgacag cgcacaggtt gcagaaattg ttgcggtaat gggtaatgcc
      601 agcgttgcca gccgtgattt aaaaattgag caatctccgg aactgagcgc caaagtggta
      661 gaaaaactga atcaggtttg cgctaaagat ccgcaaatgc tgttgatcac ggcaattgat
      721 gacaccatgc gtgctatcgg caaaaaataa tatttcatat gttaccgcgt cgatgaattc
      781 gacgcggttt tttagctaac gggcgggatc agaacttata tttcaggccc accatcattg
      841 cggtgtcatt gtagccatta tcacccagct gcacgcccac attaccccag acgctggccg
      901 ccggattcag attgccattt acgccatgtt tcatttcccc cagattacga gccccgtctt
      961 gttcgacttt cacaccgttc attgagacag caaaatcttt actgttatgg atcccgttca
     1021 tttcaacaaa aggttcaaag tcgtcaccgt gcggaccgct gtttttctcc tgagtacgaa
     1081 taaaggtgcg gaaaccaagt cggctctgga tgttgtaatt tgcgtcattc tcaatacgtg
     1141 agccattggc ttccgtgtgc gcattatgat cgacgcccat ccagatggcc tgtgccgagg
     1201 tcgttctcaa ctgtccgttg ctgtcacgcc aggcattatg cccgccaatt tgacatagcc
     1261 aaagttggct tgaacgctcc tgttcagtta caggatcgat atagcgcgtt tcacccgcac
     1321 gatcgttcag acgcgtaaca aaaagagtgt tagcggctgc caggttggcg acgtaactgc
     1381 cagcgtcggg tcgcaggacg ggggtaggcg tttctgttga ttctgaagtg acattggaaa
     1441 aaaatcactc tctaccattg tatatttttg caatgggggc tttagtgcca tttgttgtaa
     1501 tagaactttt ttatatcaat gttaccttgt cgaatagcaa ctgcata
//
LOCUS       DACADF010000333         1540 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.340, whole genome shotgun sequence.
ACCESSION   DACADF010000333 DACADF010000000
VERSION     DACADF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.340"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..610
                     /locus_tag="ISB30_004915"
     CDS             <1..610
                     /locus_tag="ISB30_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HAP1992433.1"
                     /translation="QFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIFM
                     QWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYPQ
                     YKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRITKVNNETVSY
                     FCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            610..1533
                     /locus_tag="ISB30_004916"
     CDS             610..1533
                     /locus_tag="ISB30_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP1992434.1"
                     /translation="MTLPHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRY
                     ADNRTKLPQANQIAQFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGS
                     IKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQVSQWKRYNSRTPVNLATRMALDAF
                     NVALAKDLRINIREQASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEV
                     MVKRLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQL
                     LKQMTVENVNTAVKQYLSHPVNTYTGVLLPK"
BASE COUNT          468 a          332 c          333 g          407 t
ORIGIN      
        1 gcaatttatc gaattgctcg aaaccgggac gccgaatatt gatttcagtg gtcatagtga
       61 gaacacattc cgtatcattg atttttctgt gcctcccacg gcttatggca agtttatgtc
      121 aaccattttt atgcaatggg ttaaaaacga tgtgggtgaa attttcatcc gtcagtttga
      181 aagctttgtc agccgttttt tggggaatgg gcataccagt tgtattttcc aggagtcctg
      241 caaggataat ctggttgttg aaagtaatgg agacatttac gaatgcgacc attttgtcta
      301 tccacagtac aaaattggaa acattaataa atcggaactc aaaacgatga acagtgtaca
      361 actgacagcg caaaaaaaac ggatttcagc gaaatgtcag caatgtgtat ataaacctat
      421 ctgcaatggt ggttgcccta agcatcgtat tactaaagta aacaatgaga ctgtttccta
      481 tttttgcgaa ggttataaaa tccttttttc aaccatggta ccttatatga acgccatggt
      541 ggagttagct aagaacagag taccgcttta ccacattatg gatgttgcaa aacaaatgga
      601 gaataattaa taacgctccc ccacagcacg attaacgaca atatttgggc atcgctacaa
      661 aatgctcaaa ttcaggcgtt gaaaacgctc gaccagcgtc cagcggagaa attcgcccag
      721 cagatgtatg agacgcgcta tgctgataac cgcacgaaat taccgcaagc aaatcagatt
      781 gcacagttta ctgccgcaga tgcactggct gccgatcgcc agttgttttc atctccagcg
      841 gatatcacgt ttgtcattgt cggtaatgtc gcagaagaca aactcgtggc gttaattacg
      901 cgttacttag gatcaatcaa acactctgat tcgccattag ccgcaggtaa accattaact
      961 cgcgcgacgg acaacgcatc ggttactgta aaagaacaaa atgaaccagt ggcacaggtt
     1021 tcacagtgga agcgttataa ttcccggact cctgttaatc tggcgacgcg tatggcgctc
     1081 gatgctttta acgtcgcact ggcaaaagat ctacgcatta atattcgtga acaggcatcc
     1141 ggggcataca gcgtttcttc tcgcctctcg gttgatcctc aggccaaaga tatcagtcat
     1201 ttgctggctt ttacttgtca accagaacga catgatgaac tgttaacgtt agcgaatgaa
     1261 gtgatggtta agcgtctggc caaagggatc agtgagcaag aactgaacga ataccagcaa
     1321 aacgttcagc gcagcctcga tatccaacag cgtagcgttc aacaattagc gaacaccatt
     1381 gtaaatagtc ttattcaata tgacgatcct gcagcatgga ctgagcagga gcaattgttg
     1441 aaacaaatga cggtagagaa tgttaacact gctgttaaac aatatctttc tcatccggtg
     1501 aatacctata ccggagtatt attgccaaaa taatcacagc
//
LOCUS       DACADF010000334         1512 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.341, whole genome shotgun sequence.
ACCESSION   DACADF010000334 DACADF010000000
VERSION     DACADF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.341"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..424
                     /locus_tag="ISB30_004917"
     CDS             <1..424
                     /locus_tag="ISB30_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAP1992435.1"
                     /translation="QRVSLGRNDLLIRTFPKGAFRLKGEQIDGSFLLNNETYLVEAKW
                     HSTKTGNADLHAFHGKLDQKISWARGVFISWAGFTKSGLDAWGRGKKVICVSGYDLVL
                     MLKNNISFRMLMEEKIRRAAETGNLYIKIDEIYPNISK"
     gene            552..1109
                     /locus_tag="ISB30_004918"
     CDS             552..1109
                     /locus_tag="ISB30_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017803299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992436.1"
                     /translation="MAKYHIESVQEWAPFTHNGQSYSLSHLNAHEITYKGKMQDFKFV
                     VTYGMHCFTKDGTPYNIPFKYQDARESISVCLERYEASKQLQHILPNLPSLMLYQTTE
                     EKYFTLQMMNSATNQLEPYKICVAFFKENRLMRIHVLSAFFARTGPGAPGEPIPQKPV
                     SLFKVAVDTAKKPRNSGRPKEVNNR"
BASE COUNT          462 a          306 c          349 g          395 t
ORIGIN      
        1 tcaacgagtc agcttgggaa gaaatgacct gcttattcgc actttcccta aaggcgcatt
       61 caggttgaag ggcgaacaga ttgacggcag ttttctgtta aataatgaaa cctatcttgt
      121 ggaagcaaag tggcatagca ctaaaacagg aaacgctgat ctccatgcat ttcatggcaa
      181 gctggatcag aaaatttcat gggctcgcgg cgtatttatc agttgggctg gatttacaaa
      241 aagtggtctg gatgcatggg ggaggggaaa gaaggtcatc tgtgtcagcg gttatgatct
      301 tgttctcatg ttaaaaaata acatcagttt cagaatgttg atggaagaaa aaatcagacg
      361 ggcagccgaa actggcaact tatatatcaa gatagacgaa atttatccaa acataagcaa
      421 atgatgcttc ctgtcctgtc tacaatcagc gtaactggtg ggattacagc tccacacctt
      481 gcagcattag cacttcagct tgagcggata aaaactatgg tagaattcaa aaaaaatagc
      541 tggggatgac agtggctaag taccatattg aatctgtgca ggaatgggcg ccgtttactc
      601 acaacggcca gagttactct ttgtcacatt tgaatgcaca tgagattacc tacaaaggta
      661 agatgcaaga ttttaagttt gtcgtaactt atggcatgca ctgcttcact aaagacggta
      721 cgccttacaa catccccttt aaatatcaag atgcgagaga gtcgatctcc gtttgtcttg
      781 aacggtatga agcatcaaag caactgcaac acattctgcc caaccttcca tcgttgatgc
      841 tctatcagac cactgaagaa aagtatttca cgcttcagat gatgaatagt gctacgaacc
      901 agcttgagcc atacaagatc tgcgttgcat tcttcaaaga gaaccgtttg atgcgtatac
      961 acgttctttc ggcatttttt gctcgtactg gacctggtgc accgggcgag cctatcccgc
     1021 aaaagccagt tagcttattt aaggttgctg tggatacggc aaaaaagcca agaaacagtg
     1081 gacgaccgaa ggaagttaac aacaggtgag aaagtggcgg ccccctttcg ggggcctcag
     1141 ccatttagtt tgattagata atcaagacta aacagggcag gtcgcatttg cttcgaactg
     1201 gtcgaaccgt ttccggctgc gggatgcgat tcattgctga acccctgtaa ccatccgtta
     1261 ttagtattct attatctgac gtagataagt caagtaatgt agttcttact gccgcaagga
     1321 ctttctgaag aagatagtac atcttgtcgg aagttaggag taattacttg agtaagaaga
     1381 tgtagttcta tcaggtacgc acagtgcaca caaagaaaca agttatccgc tgtgaagatg
     1441 ttcaagatag acgagcaaga ttagcttaaa aaaacagcct tagcgtaaaa taattatcga
     1501 tatccaagct gc
//
LOCUS       DACADF010000335         1503 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.342, whole genome shotgun sequence.
ACCESSION   DACADF010000335 DACADF010000000
VERSION     DACADF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.342"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(94..882)
                     /locus_tag="ISB30_004919"
     CDS             complement(94..882)
                     /locus_tag="ISB30_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HAP1992437.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            complement(1013..1486)
                     /locus_tag="ISB30_004920"
     CDS             complement(1013..1486)
                     /locus_tag="ISB30_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HAP1992438.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
BASE COUNT          380 a          387 c          371 g          365 t
ORIGIN      
        1 tagttgagcg gcgggcgcag cccgtccgct tgaacgccga gttaggcatc agatgccctc
       61 ggcgcgggtc gatgcacttt tcgcacatgc cgctcaacgc aagattctct caatcgttgc
      121 tttggcatat cgaacgaacg cggccgtctc ttcgacgcgc attgctaggt cgtcgtcctc
      181 gctacccagg tacgccgcgc gtgccttgca gatgaggggc cgatgctcgg caggcaaacg
      241 ctccgatacc catgcggcag caacgtcctt aggagcaatg agaccagttg aagcgctgta
      301 ccaaatgcga gcaagagcaa gaacgacgtt ccgctcgtca cccttccaat ccgactctgc
      361 attccactgg gcaatagtgt cgaaaagcgc cttggagaaa tgctccttcg gcaccggctc
      421 gaaaaacgtg gctgcggatg ggcctagaag cgcaaggctg tgttgcctcg ccttggtcag
      481 caaaatcgca agatcgtgat ccagaacggc aggctcgaac gttccggaaa ggatgtcgtg
      541 gcggagccac tcaccgaact gaagctcacg ccgcgccgga tagcgccaag gcactacttc
      601 gcttcgagcg acaacagtta gctccagcgg tcgccatgtt ccgccatcgc ctggcggtga
      661 tgagactttc agcaaatcga gcattagcgc ctgccggagc gaatcgttag gtgcggcgct
      721 gacggtcacg agcaagtcta tgtcgctgtc cggcttcagc cctccatcga tcgcagatcc
      781 gaacaggtgg attgtgtcca gtgtcgcagc cagatggcgc tcgatcaccg cgcgagcgtg
      841 ggacagctgc ttgaaaactt gtgcagggaa aaattcaccc atgatgccta acgttaagtt
      901 cagcggcagc ttttaagttg cggctttgtg gaatactttt gcgcagcaaa accacaaaga
      961 cgcgacttaa aagctgtcca aggagcgaag cgactggtgc tgcaacgcat tgttagcctt
     1021 ttttccaaat ctggtatgta taatttatat tagacataaa aaactgttca aaaaccaaat
     1081 tgaaattctc aggcattata gggaatttga tatcaccttc gacttcaacg tgaacagtag
     1141 acaaatgaat tatatctgct ttttcaataa ggctattata gatttgaccc ccgccagaga
     1201 catatacatg atctgtaact tttgatagct ctttcaaagc attttctatt gaaggaaaaa
     1261 ctaggacgtt ttcatttgag cttgaaattc cgttctttga cactactgca tatttgcgat
     1321 ttggaagaac acccatagag tcaaatgttt ttcttccgac aaggagccat tgattatatg
     1381 tgagcgcttt aaagagtagt tgctcacctt ttactgacca cgggatatca ggaccactac
     1441 cgattacgcc attttctgac actgcagaaa tcaatgatat tttcaattta actcccttaa
     1501 tgg
//
LOCUS       DACADF010000336         1491 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.343, whole genome shotgun sequence.
ACCESSION   DACADF010000336 DACADF010000000
VERSION     DACADF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.343"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            65..394
                     /locus_tag="ISB30_004921"
                     /pseudo
     CDS             65..394
                     /locus_tag="ISB30_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414796.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
     gene            complement(503..1435)
                     /gene="mmuM"
                     /locus_tag="ISB30_004922"
     CDS             complement(503..1435)
                     /gene="mmuM"
                     /locus_tag="ISB30_004922"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HAP1992439.1"
                     /translation="MSQNNPLRALLDKQDILLLDGAMATELEARGCNLADSLWSAKVL
                     VENPELIREVHLDYYRAGAQCAITASYQATPAGFAARGLDEAQSKALIGKSVELARKA
                     REAYLAENPQAGTLLVAGSVGPYGAYLADGSEYRGDYHCSVEAFQAFHRPRVEALLDA
                     GADLLACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAG
                     YPQVVALGINCIALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHHHGEHCAQ
                     LADYLPQWQAAGARLIGGCCRTTPADIAALKARS"
BASE COUNT          322 a          456 c          409 g          304 t
ORIGIN      
        1 gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat
       61 taccgccaac ctgttcccgg caaccttcag cgacggatac gttgatatct acggctatca
      121 tctgccgcgc ccgctgcact ttggtacaca gggtgaaggg atggtcggtg tgcgcccgga
      181 agcgatcacg ctcagcgatc gcggcgaaga gagccagcgc tgcgtgatcc gccatgtcgc
      241 ctatatgggg ccgcagtatg aagtgacggt ggaatggcac gggcaggaga tattattgca
      301 ggtcaacgct acgcgtctgc aaccggacgt cggcgagcag tattatcttg aaatccatcc
      361 gtacggcatg tttgttctgg cggatgcggc ataacgtttt gtggttttgt aggccggata
      421 aggcgcttgt gccgccatcc ggcaatgcct gatgcgctgc gcttatcagg cctacaatga
      481 acgatgtaga cccgataaac cctcagcttc gcgcttttaa cgcggcgata tccgcaggcg
      541 tggtgcgaca gcacccgcca atcaaccgtg cgccagcggc ctgccactgc ggcagataat
      601 ccgccagctg cgcgcaatgt tcaccgtgat gatgccaggt tttgctcacg gcatcgtaat
      661 gctcgcccga gttcggatac accaccagcg gcagcaccgt taaaccgtgt aaatgctgca
      721 acgcagcggt ggtgttttcc agcgcaatac agttaatgcc tagcgccacc acctgcggat
      781 aacccgccag caacgcaacc acgtcacgca gcggcgtacc gtcgctcagg tgttcgctgt
      841 cgcgcagggt aaatgagaac cacgcccgcg cacgcggata tgcggtcaac agctcggcca
      901 acgcctcaat ctcggaaaaa ttcggcaggg tttcgcaggc cagcagatcg gccccggcat
      961 ccagcaaggc ttccacgcgc gggcgatgaa acgcctgaaa tgcctcaacg ctacaatgat
     1021 aatcgccacg gtattcagag ccatccgcca gatacgcgcc gtaaggcccg acggatcccg
     1081 ccaccagaag cgttcccgcc tgcgggttct ctgccagata cgcttcacgg gctttacgcg
     1141 ccagctccac gcttttgcca atcagcgctt tcgactgcgc ttcatccaga cctcgcgcgg
     1201 cgaagcccgc cggcgtcgcc tgatagctgg cagtgatcgc gcattgcgcc cccgcccggt
     1261 agtaatcaag atgcacttcg cggataagct ccgggttttc taccagcact ttggctgacc
     1321 acaggctgtc ggctaagtta caccctcgcg cttccagctc cgtcgccatc gcgccatcca
     1381 gcagcaggat atcctgttta tcaagaagag cgcgtaacgg attattctgc gacatgttct
     1441 ggctcctgtt ttgcgtttcg gggttgagta aggaaataag caccatagca c
//
LOCUS       DACADF010000337         1468 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.346, whole genome shotgun sequence.
ACCESSION   DACADF010000337 DACADF010000000
VERSION     DACADF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.346"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            123..>1468
                     /locus_tag="ISB30_004923"
     CDS             123..>1468
                     /locus_tag="ISB30_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1992440.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQLDISNSVIHGSITSELPFGYYSITPAEVNAKGEVVTPQTYSGYNFY
                     NGITTDLDYNWYDGDVFTLNVSNSTIDDDYEALYFTDTYLDGDKSKSTNETFYTALGV
                     AVNLDVESNINITNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTP
                     LEESNGFFGKSAEPSDYAGNGGENDVALSFSDNAGSNYSMKNNVNFDHSTLLGDVEFT
                     SHWNNDGVFFYSTGHDSNGDGVLDTNGGWVDDAQNVDELNITLNNGSKWVGSANMSAE
                     VIAPADMYDVAPNSLTPGATIEANDWGRIIDNKVFQSGVFNVALNNGSEWNTVNSSV"
BASE COUNT          436 a          304 c          315 g          413 t
ORIGIN      
        1 ggaaagaata attgaaccat tcctctataa cgcagcataa tcgtaaacgc gaaacataat
       61 atgttttcta tgcattgata attgatggat caacttatta cgtccctgag gagggatgac
      121 aaatgcactc ctggaaaaag aaacttgtag tatcacaatt agcattggct tgcactctgg
      181 ctatcacctc tcaggctaat gcagcaaact atgatacctg ggggtatcat gacaacacca
      241 cgaccgattt agtttggccg tatgacactg acaatgatgg aattgccgat gctggcggca
      301 tggacccgat gggttcagac ggtaactacg taacttacaa tggttttgtt tactataaca
      361 atgccaatgg tgatttcgac accgtcttta aaggcgatac tgttaacggt accatttcta
      421 cttattactt gaatcatgat tacgctagcg gttctgttaa ccagttagat atcagcaatt
      481 cagtaattca tgggtcaatc acttctgaac tgccatttgg ttattacagc attactcctg
      541 cagaagttaa tgctaaaggt gaagttgtaa ctccacagac ctatagtggc tataatttct
      601 ataacggaat cactactgac cttgactaca attggtacga tggtgacgta ttcactctga
      661 acgtatccaa ttccaccatt gatgatgact atgaagctct ttacttcact gacacatatc
      721 ttgatggtga taaatctaag tcaaccaatg aaacgttcta taccgctctg ggtgttgcag
      781 taaatcttga tgttgaaagc aatatcaata ttactaacaa ctcccgtgta gctggtattg
      841 cgctgtctga aggcaataca agcaacacga cttatactag cgaatatcat cagtgggaca
      901 acaacattaa tgtgtctaac tccactgtaa cttcagggtc acagactccg ctggaagaaa
      961 gcaatggctt cttcggtaaa tctgctgaac caagtgatta cgctggtaat ggtggcgaaa
     1021 atgacgtagc tctgtcattc tctgacaatg ctggctcaaa ttattccatg aaaaacaatg
     1081 taaactttga ccattcaacc ctgttgggtg atgtcgagtt cacaagccac tggaataacg
     1141 atggcgtatt cttctattca accggccatg acagcaatgg tgacggtgtc cttgatacca
     1201 acggtggttg ggtagatgat gctcagaacg ttgatgaact gaacattact ctgaacaacg
     1261 gtagtaagtg ggttggctca gcaaacatga gtgctgaagt aattgctccg gcagatatgt
     1321 atgatgttgc gccgaacagc ctgacccctg gtgccactat cgaagctaac gattggggtc
     1381 gtattattga caacaaggta ttccagagcg gcgtatttaa cgtagcactg aacaatggtt
     1441 cagaatggaa taccgttaat agttcagt
//
LOCUS       DACADF010000338         1460 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.347, whole genome shotgun sequence.
ACCESSION   DACADF010000338 DACADF010000000
VERSION     DACADF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.347"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            1..495
                     /locus_tag="ISB30_004924"
                     /pseudo
     CDS             1..495
                     /locus_tag="ISB30_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1168..>1460
                     /locus_tag="ISB30_004925"
     CDS             1168..>1460
                     /locus_tag="ISB30_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992441.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLG"
BASE COUNT          359 a          368 c          438 g          295 t
ORIGIN      
        1 actgcgccgc agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt
       61 cgaccgcctc tggaaaaagt gatgccactg ctggataagc tgcgtaagct gtacggggtc
      121 ggaccggtat gcagtgaact acatattgcc ccgtcaacgt attaccactg tcagcaacag
      181 cgacatcatc ctgataaacg cagtgcccgt gcgcagcgcg atgactggct gaagaaagag
      241 atactgcgcg tatacgatgg gaatcatcag gtatacggtg tgcgtaaagt ctggcgtcag
      301 ttgttacggg aaggaatcag ggtagccaga tgtacagtgg cgcgcctcat ggcggttatg
      361 ggacttgccg gtgttctccg gggtaaaaag gtccgcacta ccgtcagccg gaaagccgtt
      421 gccgcaggcg accgcgtaaa ccgtcagttt gtggcagaac gttctgacca gttgtgggtg
      481 gctgatttta cttacggtgt gcccggagtt cagggcgggc atggatgctt aaatgaaccg
      541 cgagtctgtc tggaatattg aaccggtaac tcacgatgag aaacccaaca atcccaccgg
      601 gtgtgacggt ggagaacctg agcggcagtg acctgcggca tgcccgcagg gtgatgtaac
      661 ccgctgacaa cggggattga ggcgagatca ctaagccgag atgatcctca aggttaagta
      721 ctgaaaggct gaagaacatg aacccgttaa tccgcctctg tgggttgaaa acgtcaccac
      781 ggcctacgtg atctgacagg ccgtgcagga ggaactggca gtgatacgta agcactaccg
      841 gtcgaaggtg ttttgacatg tatgcgaaac accggggcag cagcgtctat cacgcttgcg
      901 ttgctgactt ctgccaactt gcggcaagca aggataaaga gtgcgacggg cagcctcctc
      961 agtatgcctg agtccaggca ggtaaaccgg ggaaggtcag cgacggatgt taagggggca
     1021 tggctccgat gacgcgctgg ctggcggagc ttccgtagta gtccgcgatg gggaaagccc
     1081 attacatggc gaagggaagc agtttgaatg tgtttgcgac gtgaattaac tgacctaacg
     1141 aggtgaagac ctttgataat cagcgaaatg caacgcaagc ttgccacatg ggcagccacc
     1201 gatccgtccc tacggattca acggctgctg cgtctgataa cacaaccaga atggctggct
     1261 gaagcggcgc ggatcacgct ttcatcaaag ggggcccata cccccggcgt tgatggcgtg
     1321 aacaaaacaa tgctacaggc cagactggct gttgagctgc aaatcctcag ggatgaatta
     1381 ctctcaggcc actaccagcc cttgcccgcc agacgggttt acatccctaa aagcaacggc
     1441 aaactgcgac cactgggtat
//
LOCUS       DACADF010000339         1429 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.348, whole genome shotgun sequence.
ACCESSION   DACADF010000339 DACADF010000000
VERSION     DACADF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.348"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..1322)
                     /locus_tag="ISB30_004926"
     CDS             complement(<1..1322)
                     /locus_tag="ISB30_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAP1992442.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLS"
BASE COUNT          346 a          398 c          343 g          342 t
ORIGIN      
        1 tctgacaact gaattccgcc attcttaatg ctccagccct gcgcagcagc atctccggct
       61 gtcggcaaca gggagattgt gcgtacggat gcatcttcag acggccccga tggcgtgtcg
      121 ccgccgggcg agggtttgat ttccggtgcc ttaccactaa tgaaggctaa ggtgcgaccg
      181 gctacgttca gaatagcagt tgccatacga tcgggaataa tgccacgacg cgcccatgag
      241 ctgaaatgcg tcgggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
      301 taataaccag acccggcaat atcaggatct tctgacgggt tgtttgtcgg tgtattaact
      361 ccgctaccat cggtcataaa gggaacaaaa taaatctgct gggattcttt acctttatat
      421 gcaccatata ccacttcata ttgcgtaccg tgttcttgtt tccacgcgta tgtcgtgtcg
      481 ccacaaatcc aggggactga tgccggactt ccaccgtgac actgcgccgc cagcccggca
      541 aggtcagcac ggaactgctg taccattgca agaaatgctg ctggctgctg ggcgtaactg
      601 gcattcgtca tatcgaattc cccctgcatc cagcatatcg ccagcaaaac gtttttcggg
      661 tttttctgca atgctgcctt cgtgcggaaa agcagatcct gatataacgg cttacccact
      721 ccccagcgag ccgaatcctg actggccccc gtggactcgc tgaatgtccc ctccgtgccc
      781 tgggtgaatg ccgaaccacc acgacagcat ggtaccagca ggatccccgc attattaggg
      841 atatacggaa gcagtttttt ggcaatatgt aagccctgtc cgacacagcc gtactgccct
      901 ttgctcaggt cagcccgggg atggttaatc gtactcatat cctgaacatc atgcagacaa
      961 tggtcagcag gaatgatgtc gttaaatacg catacttcac caccgggagt cactgtgtta
     1021 cgacgggcca gttgcttaat gcgcggatgg ggcgcatcgt atgaatccgg aagcggaagc
     1081 ccttcaccgt aagccatggc attggattgc ccggccagta cgatgacgta gtaccactcc
     1141 ggctcagttg caccactgac gaccacatca ccttctgctg caatcgcctg catcagggta
     1201 taaggggtta tggccaccgg actaccaaac ggctgccagc cctctttcag tttatgtgtc
     1261 agcttttccg caagatctga cggcgacgcc gccctgacaa catcatagtg tttaaatgcc
     1321 atggttcttt ccaccatctg aaaaatgatt ctttaaaata cctgacatgt aatacagaaa
     1381 aaacacaaaa ccatacctta aataaaaacc tcatcatcaa gcagatatg
//
LOCUS       DACADF010000340         1414 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.349, whole genome shotgun sequence.
ACCESSION   DACADF010000340 DACADF010000000
VERSION     DACADF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.349"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            144..1355
                     /gene="bluF"
                     /locus_tag="ISB30_004927"
     CDS             144..1355
                     /gene="bluF"
                     /locus_tag="ISB30_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAP1992443.1"
                     /translation="MLTTLIYRSHIRDAEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKEGEIYTAD
                     LKSKALAFAMAHALKLGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
BASE COUNT          398 a          297 c          303 g          416 t
ORIGIN      
        1 ggttttttca ccaaaccgtc ttcaaccttt tccacttttt atcaatgtaa agaaacggtt
       61 atgttttggt attaaaagaa agacacaaga agatatgtct gttaccgtct tactctcgcc
      121 tcgcccatta ccctggattg ttaatgctta ccacccttat ttatcgtagc catatacgtg
      181 acgccgaacc tgtcaaaaaa atcgaagaaa tggtttcgat agcaaatcgc aggaacatgc
      241 ggtctgacgt aacagggatc ttactgttta atggttctca ttttttccag cttctggaag
      301 gtccggaaga acaggttcaa atgatatatc gggccatatg ccaggatcca cggcactata
      361 atattgttga actgttgtgc gattacgcgc ctgctcgccg ttttggcaaa gcaggaatgg
      421 aattatttga tttacgcctg cacgagcgag atgacgtttt acaggccgta ttcgacaaag
      481 gcacatcaaa atttcagcta acttatgatg acagagcgct acaatttttt cgtacttttg
      541 tccttgcaac tgaacaatca acctatttcg agattcctgc tgaagactcc tggcttttta
      601 tcgctgacgg atctgataaa gaacttgatt cttgtaccct ttcatcaaat ataaacgacc
      661 actttgcctt tcatcctatt gtcgaccctt tatcgcggcg gataatcgct tttgaagcca
      721 ttgtgcaaaa aaatgaagac agcccgtcag ctatagcggt tgggcagcgt aaagaagggg
      781 aaatctacac agcggatctc aaaagtaagg cgcttgcatt cgcgatggca cacgcactta
      841 agctcggtga taaaatgatt tcaatcaatc tattacctat gaccctggtt aacgagcctg
      901 acgcagtctc ttttttactt gatgaaataa aggccaatgc tctggtgcct gaacaaatca
      961 tcgttgaatt tactgaaagt gaagtcatat ctcggtttga tgagtttgcc gaagcggtta
     1021 aatcgctaaa ggctgccggt atcagtgtag caattgatca ttttggcgca ggttttgctg
     1081 gtttgttact cctgtcacgc ttccagcctg acagaattaa aatcagtcag gaattgatta
     1141 ccaacgttca taaaagcgga ccacggcagg caattattca ggcgatcatg aaatgctgta
     1201 catcacttga aattcaagtc tctgctatgg gcgtggcaac accagaagag tggatgtggc
     1261 ttgaatctgc aggaattgag atgtttcagg gagatctgtt ttccaaagct aaattgaatg
     1321 gtatcccttc agttgcgtgg ccggagaaaa aataattttc agcacattat ttcgcatgat
     1381 ttctggaaat catcgtgaaa atgaatacgt tatg
//
LOCUS       DACADF010000341         1403 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.350, whole genome shotgun sequence.
ACCESSION   DACADF010000341 DACADF010000000
VERSION     DACADF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.350"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..1319)
                     /locus_tag="ISB30_004928"
     CDS             complement(<1..1319)
                     /locus_tag="ISB30_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HAP1992444.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLV"
BASE COUNT          382 a          348 c          283 g          390 t
ORIGIN      
        1 actaatgaaa atagatcatt gagtgattcg ctcatcgacg gatgagtaaa tatctgatcg
       61 cgtaatatgc tataaggcag cccggcatcc atcaccattt tcactatatt gatcatctcg
      121 tgggagtcaa cacacagcag tgatgcccct aatatacgtt gggttttatt atcaacaatc
      181 gcttttaata ccccacgagt atcattcatc actctggcac gcggaattgc agctacaggc
      241 aatgtcacca cctgaatatc agcaccactc tctctggcct gttcttctgt cataccaacc
      301 ctggacagtg gcggtgtcat gaatacggaa taaggcacat ttttccgatc atcagtacta
      361 cgtctgcctt ctcccagcaa ctcatcacgc acaatgcggt aatcatccag tgaaatataa
      421 gtaaattgca gcccgccggt aacatctccc atcgcccaaa tattgtctgc ggtggtatgt
      481 aattgcttgt caacgacaat tgccccgcgt tcgtttactg cgatacctgc attttctgga
      541 tgtaacgaag cggtagccgg ttgacgaccg gaagctatta acagtgcatc caccgccagt
      601 tgggcgtgtt cgctatgcac ttgcacttga ttttcatggt gactgattcg ctccacatgg
      661 gcattgagga ttatatcgac gccctgatcg cgtaaaatcg tcgcgatatt atcagcaata
      721 tcccgatctt cccgaggcaa aaacagcgaa gctgcctcta aaatggttac tttgctgcca
      781 aaattagcga acatagaggc gaactcaacg ccaatatatc cgccgcccaa aatacctaaa
      841 tgcccaggca attcttttag attaagtaat ccggtgctgt catatactcc tggcgtggtg
      901 gtaattccag gaattggcgg aaccacggtt tgtgcaccgg tattaataaa aattttctcg
      961 ccatgaattt ccagatttcc ctcaggccga tgaacacgca ggctatgatt attgataaac
     1021 tccgcctggc cgtcgatcac gtcgatattg ggcatatccg caagattatg aaaattctta
     1081 ttacgtaaaa aattaaccac ttcattttta cgctgtatgg cacggacaaa atctgtgtgc
     1141 tgctgtgcgt catgaaccaa tgttttggtt gggatacagc cgatattaat acaggtcccg
     1201 ccatacattg catttgattg ttcgatgaga gccacacgcc aacctgcttt tgccagcgtg
     1261 acggctaatg tttttccagc cttgccaaaa ccaataataa ctgcctgata tttattcatg
     1321 atgatttcct gtcgaaacgc gttcatttga catcatgact atagacgcaa aagagcaaat
     1381 aacatttctt cacaacgctt gat
//
LOCUS       DACADF010000342         1380 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.351, whole genome shotgun sequence.
ACCESSION   DACADF010000342 DACADF010000000
VERSION     DACADF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.351"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(137..1180)
                     /locus_tag="ISB30_004929"
     CDS             complement(137..1180)
                     /locus_tag="ISB30_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992445.1"
                     /translation="MEKGTIFPAMGYLHIDGRGMKPNGGSRYNPLEAETIAAWLVAHK
                     DDIERHYGEPLYKVVGVVTPFSAQVNAIKTSLRKLEINGKDEQGSLTVGTVHSLQGAE
                     RAIVLFSPVYSKHEDGRFLDSNSSILNVAVSRAKDSFLVFGDMDLIEMQPAFSPRGLL
                     AKYLFSSDNNALQFEFQKRQDLISAHTQISTLHGVEQHDGFLNKTLAGAQKKITIISP
                     WLSWQKVEQTGFLASMALARSRGIDITVVTDKNCNIAHVDDDKRQEKQHLLNDAVEKL
                     NKMGIATKLVNRVHSKIVIEDEELLCVGSFNWFSAAREDKYQRYDTSLVYRGEGVKNE
                     IKAIYGSLDQRQL"
     gene            1306..>1380
                     /locus_tag="ISB30_004930"
     CDS             1306..>1380
                     /locus_tag="ISB30_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1992446.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTV"
BASE COUNT          396 a          339 c          261 g          384 t
ORIGIN      
        1 ttatgcaaag gaaaatgatt aggtaaacaa taagatttat aatcaggaaa ggcgactcgc
       61 aataaaacgg aaagtcgcct taacattggt aaaatatgcc ctttactact cctgtatcat
      121 gaacggctca tcacatttaa agctgacgtt gatccagact accatatatt gcctttattt
      181 cattttttac gccttcgcca cggtacacca aagacgtatc gtatcgttga tacttatctt
      241 cccgtgcagc actgaaccag ttaaaagatc cgacacacag cagttcttca tcctcaatca
      301 caattttgct gtggacacga ttaaccaatt tcgtagcgat acccatctta ttgagctttt
      361 caacagcatc attcagcaga tgttgtttct cctgacgctt atcatcatcg acatgcgcta
      421 tattgcagtt tttatccgtc accacggtaa tatcaatgcc ccgggaacgg gccaaagcca
      481 ttgatgccag aaatcccgtt tgctccactt tttgccaaga taaccaaggg gaaataatcg
      541 ttattttctt ttgagctccg gcaagagtct tgttcaaaaa tccatcatgc tgctccacac
      601 catgcagcgt tgaaatttgt gtatgtgcgg aaattaaatc ctgtcgtttc tgaaactcaa
      661 attgcaacgc attattatct gaagaaaaga gatattttgc caataaccct cgcggcgaaa
      721 atgcgggctg catctcgata aggtccatat cgccaaatac cagaaaacta tctttagcgc
      781 gtgagacagc aacattgagg atactgctgt tgctatcaag aaacctgcca tcttcatgtt
      841 ttgagtagac aggagaaaag agaacaatcg ccctttccgc cccctgcaag gagtgcacag
      901 tccccactgt cagtgaaccc tgctcgtctt tgccattaat ttccagctta cgcaatgacg
      961 ttttgatagc attaacctgc gccgaaaatg gcgtcacaac accaacaact ttgtacagtg
     1021 gttccccgta atggcgctct atatcatcct tatgtgccac cagccatgct gctattgttt
     1081 cagcttcaag agggttataa cggcttcctc catttggctt catgcctcta ccatcaatat
     1141 gtaaatatcc cattgcgggg aaaatcgtcc ctttttccat ccctctttta ggctgcaact
     1201 taccgtgata gcaaagtgag ttacagtagc cgatgaagtg gtttactgaa tttggccacc
     1261 tgaacagagg tgatatgctc acctcagaac aacacaggtg ccataatgaa aaaaagaaat
     1321 ttcagcgcag agtttaaacg cgaatccgct caactggtcg ttgaccagaa ttacaccgtg
//
LOCUS       DACADF010000343         1377 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.352, whole genome shotgun sequence.
ACCESSION   DACADF010000343 DACADF010000000
VERSION     DACADF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.352"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..714
                     /locus_tag="ISB30_004931"
     CDS             <1..714
                     /locus_tag="ISB30_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP1992447.1"
                     /translation="KSMLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHR
                     LHALGAHLSAEERQAVADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRR
                     FHIEEENASGPMAQVIGRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVL
                     VDNIRWQSYLSSMTSAEAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVL
                     KPAVLERQRKSKGVPRGEA"
     gene            701..>1377
                     /locus_tag="ISB30_004932"
     CDS             701..>1377
                     /locus_tag="ISB30_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C"
                     /protein_id="HAP1992448.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYD
                     YGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWE
                     AVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRL
                     LSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWI
                     DPGKTGKA"
BASE COUNT          252 a          441 c          481 g          203 t
ORIGIN      
        1 aaatccatgc tggccctgca actggccgca cagattgcag gcgggccgga tctgctggag
       61 gtgggcgaac tgcccaccgg cccggtgatc tacctgcccg ccgaagaccc gcccaccgcc
      121 attcatcacc gcctgcacgc ccttggggcg cacctcagcg ccgaggaacg gcaagccgtg
      181 gctgacggcc tgctgatcca gccgctgatc ggcagcctgc ccaacatcat ggccccggag
      241 tggttcgacg gcctcaagcg cgccgccgag ggccgccgcc tgatggtgct ggacacgctg
      301 cgccggttcc acatcgagga agaaaacgcc agcggcccca tggcccaggt catcggtcgc
      361 atggaggcca tcgccgccga taccgggtgc tctatcgtgt tcctgcacca tgccagcaag
      421 ggcgcggcca tgatgggcgc aggcgaccag cagcaggcca gccggggcag ctcggtactg
      481 gtcgataaca tccgctggca gtcctacctg tcgagcatga ccagcgccga ggccgaggaa
      541 tggggtgtgg acgacgacca gcgccggttc ttcgtccgct tcggtgtgag caaggccaac
      601 tatggcgcac cgttcgctga tcggtggttc aggcggcatg acggcggggt gctcaagccc
      661 gccgtgctgg agaggcagcg caagagcaag ggggtgcccc gtggtgaagc ctaagaacaa
      721 gcacagcctc agccacgtcc ggcacgaccc ggcgcactgt ctggcccccg ggctgttccg
      781 tgccctcaag cggggcgagc gcaagcgcag caagctggac gtgacgtatg actacggcga
      841 cggcaagcgg atcgagttca gcggcccgga gccgctgggc gctgatgatc tgcgcatcct
      901 gcaagggctg gtggccatgg ctgggcctaa tggcctagtg cttggcccgg aacccaagac
      961 cgaaggcgga cggcagctcc ggctgttcct ggaacccaag tgggaggccg tcaccgctga
     1021 tgccatggtg gtcaaaggta gctatcgggc gctggcaaag gaaatcgggg cagaggtcga
     1081 tagtggtggg gcgctcaagc acatacagga ctgcatcgag cgcctttgga aggtatccat
     1141 catcgcccag aatggccgca agcggcaggg gtttcggctg ctgtcggagt acgccagcga
     1201 cgaggcggac gggcgcctgt acgtggccct gaaccccttg atcgcgcagg ccgtcatggg
     1261 tggcggccag catgtgcgca tcagcatgga cgaggtgcgg gcgctggaca gcgaaaccgc
     1321 ccgcctgctg caccagcggc tgtgtggctg gatcgacccc ggcaaaaccg gcaaggc
//
LOCUS       DACADF010000344         1370 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.353, whole genome shotgun sequence.
ACCESSION   DACADF010000344 DACADF010000000
VERSION     DACADF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.353"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            7..>1370
                     /locus_tag="ISB30_004933"
     CDS             7..>1370
                     /locus_tag="ISB30_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAP1992449.1"
                     /translation="MNKKLKKTASGAITWSVIVTQILSPVSLSLIPANSFASSGNKDV
                     TQIYASDEHANKVASFAASAGQSLANNNASSFAVNTLSTQATKEVVDWLQQYGNARIK
                     LNVDDSFSLKDSSFDFLYPWLDTQDYVLFSQTSLHRTDDRNQTNIGLGIRHFTPDNAM
                     LGANVFYDYDLSRSHSRAGFGVEYWRDYFRLGVNTYFGLSDWKNSRDIDDYLERPANG
                     WDFSAEGWLPAYPQLGASIQFEKYYGKNVGLFGSDNLQENPYAVTGGISYTPVPLIKF
                     SAQHKQGQSNTHDTTFGVEFNYRPGVSLAEQLSSDNVAVMREVQNRRYDFVERNNNIV
                     LEYKKKHALKISLPESVQGEGESIIPVTLTINNASGGIKSVQWNDSAFTAAGGKISGN
                     GTSWQITLPAYKSEGVNSWNVGATVQDNRGNVSNYAVMNISVTDSGVSTADSSFTLDG
                     DSSPT"
BASE COUNT          385 a          257 c          327 g          401 t
ORIGIN      
        1 accactgtga ataaaaagtt aaaaaaaact gcatctggcg cgattacatg gtcggtaatt
       61 gttacccaga tattatctcc agtttctctt tctttgatcc cagcaaacag ttttgcatca
      121 tctggtaata aagatgttac gcaaatttat gccagcgatg agcatgcaaa taaagtggcc
      181 tcatttgcag caagtgcagg tcagagcctg gcgaataata atgcaagtag ttttgctgta
      241 aatactttat caactcaggc gacaaaagag gtcgtcgact ggttgcaaca atatggcaat
      301 gcgcgcatca agcttaatgt cgatgattct ttttccttga aggattcatc attcgacttt
      361 ttatatccat ggctggatac tcaggattat gtgttattca gtcagacatc actacatcgt
      421 actgatgacc gaaatcagac caatattggt ttggggattc gtcatttcac tcctgataac
      481 gcaatgttgg gtgcgaatgt tttctatgat tacgatttaa gtcgtagtca ctctcgtgca
      541 ggttttgggg ttgagtactg gagggattat ttcaggcttg gtgtaaatac ctattttggt
      601 ctatctgact ggaagaacag tcgtgatatt gatgattatc tggaaagacc tgcaaatggc
      661 tgggattttt ctgctgaagg atggctacct gcttatccgc aattaggggc atctattcag
      721 tttgaaaaat attatggtaa aaatgtcggg ttatttggaa gcgataacct gcaggaaaac
      781 ccttacgcag ttactggggg aatttcttat acaccagttc ctctgattaa gttttctgca
      841 cagcataagc aaggacagag caacactcac gatacaacct ttggtgttga gtttaattat
      901 cgcccgggcg tttccttggc tgaacagctt agcagcgata atgttgcagt tatgcgagaa
      961 gtccagaacc ggcgttatga ttttgttgaa cgaaataaca acattgttct ggagtacaag
     1021 aagaagcatg cactgaaaat cagtttgcca gagtctgttc agggggaggg ggaatcaatc
     1081 atccctgtaa cactgacaat caacaatgct agtggtggta tcaagtctgt acagtggaat
     1141 gatagtgcat tcactgcggc tggcggcaag atcagtggaa atggcacatc atggcagatc
     1201 actttaccgg cttataaaag cgaaggtgta aattcgtgga atgttggagc cacagtacag
     1261 gataacagag gcaacgtttc caactatgcc gtgatgaata ttagcgttac tgatagtggt
     1321 gtctcgacgg ctgattcgtc ttttacactg gatggagatt cgtcgccgac
//
LOCUS       DACADF010000345         1355 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.354, whole genome shotgun sequence.
ACCESSION   DACADF010000345 DACADF010000000
VERSION     DACADF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.354"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..824)
                     /locus_tag="ISB30_004934"
     CDS             complement(<1..824)
                     /locus_tag="ISB30_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP1992450.1"
                     /translation="MPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGKPQVYGV
                     HTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPGHTGNDR
                     PPLEQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVYLKEDPR
                     KLPGVVTGHGVPLSPGTRWLDMSVSNNGNGAPIPAHIVDKLRGREFKTFDEFREALWL
                     EVSQEPELIAQFSEINQLRISQGFTPFAPDEGHYIGPKETLKKFQIHHFIAIEYGGG"
BASE COUNT          334 a          355 c          339 g          327 t
ORIGIN      
        1 ccgccgccat attcaatagc aataaaatga tgtatctgaa attttttaag tgtttcttta
       61 ggaccaatat agtgtccttc gtcgggggca aaaggtgtaa agccctgact aatacgtaac
      121 tgattaattt ccgaaaactg cgctatcaac tccggttcct gactcacctc cagccacagc
      181 gcctcgcgaa actcatcaaa ggttttaaac tcccgtccgc gcaatttatc aacaatatgc
      241 gccgggattg gtgcgccgtt gccgttattg gataccgaca tatccagcca gcgagttcct
      301 ggtgaaagcg ggacgccgtg ccctgttaca acaccaggca gctttcgcgg atcctccttc
      361 aggtaaacat agataggctt aataccggat cccgccggaa aaaccagaat ataatcgttg
      421 aattctttcg gatcaggaac cgggaatggc ggcgtggtat aggtatcggt gccgtccgga
      481 atcggcgtca ccagaatggt gggctgctcc agcggcggcc tgtcgttacc ggtatggccc
      541 ggaacattcc catgttccgg attttccggc gtccaggtaa tcagggggcc atcgacatcg
      601 tgagcggggg taaattcata acgctgcgtc tgctcgttcc actgcatgtt cgccacgcgg
      661 acgttttcat acggcgtacc ttcacccgta tgaacaccat acacctgagg cttgccgtga
      721 atgtcagttc cccagaaaaa acgcacccgg gtggtggcgg tactgcctgc tagtgctttc
      781 tgccgcagtt cttcctcata ccagaactca tcacgtccag gcacacgagc actgccctca
      841 cccgcagagg gaatatacag agcagatgcc aacgcaccga tgtaagagcc gcgggttgca
      901 ctgagtacgc cactgagcga ccattttccc catatttgag tcgccaacac actactcggg
      961 aacagtagca tggtttgagc ttctgcgggt tccccgataa cgttagcctc gggatggtgg
     1021 tatccacagc agtcatcaac cactggctga acgaaaatcg ctacttgacc gaagttttca
     1081 gcgggttctt ctgccgttcc ggcatctgta catcccttca ccttcagaca ggatttggca
     1141 tagaccgggt taaccgctgg tgaagagagt ggaaggttaa caactggtag cggttcaggc
     1201 ttaagattac tggcaggtgg acttttaggc ggaataaata agctattata gccagcagga
     1261 aaggccaacc agtcaaattc cgtattggct atgagatgtt tacataatat gtagtcataa
     1321 tcgacagtta cacattcggt aggaagatta ttgtc
//
LOCUS       DACADF010000346         1347 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.355, whole genome shotgun sequence.
ACCESSION   DACADF010000346 DACADF010000000
VERSION     DACADF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.355"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            146..>1347
                     /locus_tag="ISB30_004935"
     CDS             146..>1347
                     /locus_tag="ISB30_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAP1992451.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRAFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSPSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANVVS"
BASE COUNT          368 a          293 c          304 g          382 t
ORIGIN      
        1 cggtgacctg gggctaacct gataatcata ataaatgtaa tgtatgccat ttagggctat
       61 gcccgcctga ttcagtgcta cgcattgaat caggttcttt tcgcgttgaa caatatcaag
      121 atacggctat tcgcagggag tggatatgtt taccaacgta aatgttgatt gttgcaaaac
      181 accaggatgt aaaaacctgg ggttgctgaa tagccaggat tatgtcgcac agggtaaaaa
      241 tattttatgc cgtgaatgtg gttacttgtt tccagtgata tctgaacagt cgcttaatat
      301 ttatcgtaat attgtgaatc actcctggag aggtttgatt tgccaatgtt caacttgcgg
      361 aggcacgtcc ctcaaaaaat atggatattc tgcacaaggc cagagaagaa tgtattgcca
      421 tcattgtgag aaaacattta tcactctgga acatgtaatt accacaccac gaggagccca
      481 gttagcattg atgattgagc aaggggaggc acttgcggat atcagaaagt cattacttct
      541 taacagcact ggacttagcc gtgaactgtt aaaattagcg cgtgaagcaa actataaaga
      601 aagtcgacag tgtttcccag cttctgatat taccctgagt acccgcgctt ttcgcgtcaa
      661 gtataatggt agcaataact ctctttatgc tcttgttacc gcagaagaac aaagcggcag
      721 ggtggttgcc atctcaacca attactcccc atctgccgta gagcaacatt atcaatacac
      781 atcgaactat gaagagcgta tgtctccagg gacgctggca catcatgtcc agcgcaaaga
      841 gttacttact atgcggcggg ataccttgtt tgatattgat tacggcccgg cagttttaca
      901 tcaaaacgat ccgggaatgt tggtaaaacc ggttcttccg gcatatcgtc attttgaact
      961 ggtcagaata ctgaccgatg agcattccaa caacgttcag cattaccttg atcacgaatg
     1021 ctttatattg ggcggctgcc tgatggctaa tttgcagcat attcatcaag gtcgctgcca
     1081 tatttccttt gtcaaagagc gcggtgtggc acccgccacc attgattttc cgccgcgatt
     1141 attccttagt ggtggggtac gaaataatgt ctggcgtgca ttttctaacc gcaattattc
     1201 aatggctgta tgcaatctca ctggcagtaa gaaagtccgc gagatgcggc atgcaacatt
     1261 gaacagtgcg acgcgtttta tccactttgt ggagaaccat cctttcctta tatcattgaa
     1321 ccgaatgtct cctgcgaatg tcgtttc
//
LOCUS       DACADF010000347         1324 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.356, whole genome shotgun sequence.
ACCESSION   DACADF010000347 DACADF010000000
VERSION     DACADF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.356"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            141..899
                     /locus_tag="ISB30_004936"
     CDS             141..899
                     /locus_tag="ISB30_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAP1992452.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(952..1059)
                     /locus_tag="ISB30_004937"
     CDS             complement(952..1059)
                     /locus_tag="ISB30_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992453.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT          340 a          291 c          307 g          386 t
ORIGIN      
        1 aaaaatgaaa taagaataca ttgctaagtt attcgcgcct ttttataatt cgttaccttt
       61 ccagggacgc taatctttat ccttaagaaa gtttccggga ccgcgatggt ctcttttttt
      121 gttgtacgga ctcttgataa atgaagcttt tgaagacagt tcctgccatc gttatgttgg
      181 cggggggcat gtttgcgtca ctgaatgcag ctgccgatga ttccgttttt actgtcatgg
      241 acgatcctgc cagcgctaaa aaacctttcg aaggtaatct gaacgcagga tatcttgcac
      301 aatcaggcaa tacgaaaagc tcctcactta cggctgacac cactatgacc tggtatggac
      361 aaaccactgc ctggtcgctg tgggggaacg ccagtaatac ttcttctaat gatgagcgtt
      421 cttcagaaaa atatgcggcg ggcgggcgta gccgttttaa tttaactgat tatgactatt
      481 tatttggtca ggcaagctgg ctgacagacc gttataacgg ctatcgagag cgcgatgtgt
      541 tgaccgcggg ttatggtcgc cagtttttga atggcccggt acacagtttc cgctttgaat
      601 tcggccctgg tgtgcgttac gacaaatata ccgacaatgc cagtgaaacc cagccgctgg
      661 gctatgcctc cggggcttat gcatggcagt tgactgacaa cgctaaattt acgcagggtg
      721 tttcggtctt tggcgcggaa gacacaacgc tgaactccga aagcgcatta aatgttgcta
      781 ttaatgaaca ttttggactg aaggtggctt acaacgttac atggaactct gaacctccag
      841 agtctgcacc ggaacatacc gatcgtcgga caaccttatc gttgggttac agcatgtaaa
      901 attatctgcc ggaccgtgtt atgcggtctg gctgttttcg tttgtcactg ctcagcccca
      961 aatcattgct gcccaacgta gcaacaacat agcgccacag attgccagta gcaccaccac
     1021 catcttccag tgttttttat caaagcgttt agtcggcata ccagtgtcct gcatatctct
     1081 tgtgcgtgat aacggagtgt aacaaaaggg acaggcatca agggattttt tgttattaca
     1141 gtcgtcgggt ggcgttgtac acctggtcac tgtttctgca ttttatgaaa aaaattatgt
     1201 atcgatatac aaatatcata aatagcgggt tataaatacg tctttagttc atcagcacta
     1261 aaactcaggc tttggctgat tacatttata gcagcataat atgtttctac agttttatga
     1321 tgat
//
LOCUS       DACADF010000348         1298 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.357, whole genome shotgun sequence.
ACCESSION   DACADF010000348 DACADF010000000
VERSION     DACADF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.357"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            159..761
                     /locus_tag="ISB30_004938"
     CDS             159..761
                     /locus_tag="ISB30_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP1992454.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(800..1225)
                     /gene="nudI"
                     /locus_tag="ISB30_004939"
     CDS             complement(800..1225)
                     /gene="nudI"
                     /locus_tag="ISB30_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAP1992455.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     RDVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          360 a          286 c          274 g          378 t
ORIGIN      
        1 tattttttct tatttagact ttgcatttgg caaaattttg aggcattttg ccggcatcat
       61 aggattttta atattacacc aactgctaat tatcgccaga aaagtcgctc aatttcacgg
      121 taattgtctg gttgcgcttg tctacaggtg gagtttacgt gttagctttt tgccgatctt
      181 cgttgaagtc aaaaaaatat ttcatcattt tactggcgct cgctgcaatt gccggactgg
      241 ggactcatgc cgcctggagt agcaatggtt tgccacgcat cgacaataaa acactggcca
      301 gactggcaca gcagcacccg gttgtcgttt tgtttcgcca tgctgaacgt tgcgatcgtt
      361 caaccaatca atgcttgtca gataaaacag gtattacggt caaaggtacc caggatgccc
      421 gtgaactggg caacgctttt agtgctgata tccctgattt tgatctctat tccagtaata
      481 ccgtccggac tattcagtcg gcgacctggt tttcggcggg taaaaagctg acggtagata
      541 aacgatttct tcaatgcggt aatgagattt atagtgcaat taaagactta caacgcaaag
      601 cacctgataa aaatatcgtt atttttaccc ataatcattg cctgacatat attgctaaag
      661 ataagcgtga tgcgacattt aaacctgatt atctggatgg tttagtcatg catgtggaaa
      721 aaggcaaagt ttatctggat ggggaattcg ttaaccacta aattttcaat ctgacagcca
      781 gtgatggctg tcatcattgt taaagaagac ctttcaaacg taacgttttt cgggtggcaa
      841 cattcaaatc ataatgcacc agatcttcag gttttaccca cgcgtagtcc tgaaactctt
      901 cgtttatttt cacatctcgg ttggcagaaa cgcagtcaaa aatcaggtaa atcatataaa
      961 tctcttcctt gcgaccatct gcatacgtct tggtgcgaat atcatcgctg aaggtccacg
     1021 gcgtgatttc tgtcaaaagc agctgttctc ccagttcttc gcgaatttcg cggcgtagtg
     1081 cctcttcaat tcgttcgcca ggctccacgc cgccacccga aagcgcccat tgaccgggga
     1141 aaacgccgcg atcgtcggcc attttacaca gcaaataagc accatcattt tgaatcaaag
     1201 ggcatacaat agtccgttgt cgcacagggg tattccttat aaataaaaag aaaccatgag
     1261 aatacggtaa ctcagaaata tgtgaaagtg acagtaat
//
LOCUS       DACADF010000349         1281 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.358, whole genome shotgun sequence.
ACCESSION   DACADF010000349 DACADF010000000
VERSION     DACADF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.358"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            294..557
                     /locus_tag="ISB30_004940"
     CDS             294..557
                     /locus_tag="ISB30_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP1992456.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            557..697
                     /gene="rpmJ"
                     /locus_tag="ISB30_004941"
     CDS             557..697
                     /gene="rpmJ"
                     /locus_tag="ISB30_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP1992457.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(767..957)
                     /locus_tag="ISB30_004942"
                     /pseudo
     CDS             complement(767..957)
                     /locus_tag="ISB30_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          364 a          288 c          271 g          358 t
ORIGIN      
        1 tatatccgtg ggagaggaat acacattgtc aggtaatcaa tcatgctgca ataaatcatc
       61 ggccagtaaa gtggagatag cctccattct cgaaaaatcc atactctcag cgaaaccatc
      121 atcaatcact catccaggcg tttatgggag cgtcgccaat ggctgctaac aatgccagac
      181 ttccccgttg cggaaattcc acatcccaca aatagtcaca gtgattgggt gttgaaatga
      241 tccggatgag catgtatctt tacggttatg ttataacata acaggtaaaa atgatgaaac
      301 ccaatatcca tcctgagtat cgtactgtgg tgttccacga caccagcgtt gatgagtact
      361 ttaaaatcgg ctcgactatc aaaacagacc gtgagattga gctggatggc gtaacgtatc
      421 cgtacgtaac aattgatgtc tcttcgaaat cgcacccgtt ctatacaggg aagctgagaa
      481 cagtggcatc agaaggaaat gttgcacggt tcacccaacg ttttggtcgt tttgttagca
      541 cgaaaaaggg ggcatgatga aagtccttaa ctctctgcgt accgcaaaag aacgccatcc
      601 agactgtcag attgtgaagc gaaaaggacg gctgtatgtg atttgtaaat ctaatccacg
      661 ttttaaggcc gttcagggtc gtaagaaaaa acgttgattt aaaattcgac gaattaacga
      721 tatttgtctg attaataatc agaccggatt aatgttggtg tatttattac accagcatta
      781 attttcctgg ggatgtatct tcctgttcat ttaaggtcaa ctgcctgacg tttctctccg
      841 aatattccat tatattaatg ttgacttgtt gaccagcttc gccctgtatg ctggcatcaa
      901 ctctctttta gactgaacac gccactcagt ttcctccctt tgcgacgcag cctgcattta
      961 cactcaaact gttaagatga taaatgtggt aaatctgttg gtactgacat aaaaacgttt
     1021 acgccacagg aacggccaga tccatcggta accccatcgc cgacgttcga gtgccagtta
     1081 gagtaacgcg cacagatgac tgaatgcagt gccctggcaa agaggccatc gttcctgtga
     1141 caactggcag tcttcgttta acttcactta atttagctct tagggggctt accggacaga
     1201 tgacgtactt acacctgttt aattttccat cacttattgg aatgaacacc cataaccatt
     1261 ttgtgcggca tgttaatcca t
//
LOCUS       DACADF010000350         1258 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.359, whole genome shotgun sequence.
ACCESSION   DACADF010000350 DACADF010000000
VERSION     DACADF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.359"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(7..900)
                     /locus_tag="ISB30_004943"
                     /pseudo
     CDS             complement(7..900)
                     /locus_tag="ISB30_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT          384 a          280 c          254 g          340 t
ORIGIN      
        1 caattttcac tcaaccacaa ccttcacctt cggtttagaa aggataattt tgaccaggtc
       61 gatgtatgta acaggtttat agaaataata accctgctga aatgtaatat tattttggtt
      121 cagatagtca agttgttctt tcgtttcgac accttcagcg acgatactga tcgaaagttt
      181 acgcgccaga tccaatacac aatccagaat tcgggttgat tctgggtctg cgttgacgcg
      241 gccaacgaaa ctgtgatcaa tttttatata atcaatatgc aggtcatgca gataagaaag
      301 ccctgagtaa ccagtaccga aatcatccag cgcgatgaca aaaccatttt catgcagaat
      361 gttcaaccgc tgaaccagac tttcatcaac atttaatggt tcacgctcgg tgacttccag
      421 aacaaggttt aaatcgcggc gggtaaaact gtcacggtaa tttaagcact cgtcgacaaa
      481 cgtcggcgaa acaatatgcg aggcgctaaa attaatccca atatgaaaac cttccggcag
      541 tttactcgcg atagcgttca tctgggtggc aacctgattc atcaggcttt gcgtaagcgg
      601 aacgattaat ccgcattttt cagcaagtgg aataaatgcc gcaggtgata tatatccacc
      661 gtgaggttgt ttccagcggg ctaacacctc aactccccgc aatgtccctt cccgaccatt
      721 taccacaggt tggtaaaaag ggacgatctc cccattattt atagcccgtc gcaggatctc
      781 ttcaggcgtt gcgcttttat tgaaatacct atcaagaagg aaggcagcgg cgcaggcgat
      841 taacagaata aatatcaaaa tcccaaagcc tttatcgata agtctgtaga agctaaagag
      901 acgttcattc gctcgcgctg gggtctacag atagcatggt agagagcatc acaaatatcc
      961 acgccgaaga taaaattttc tccaacttca tctaacctat taattattta gaacaatgta
     1021 ggcagaatta aacaaattag gcgggatatc aggcgtcaag aatggaaaac gagcgctctc
     1081 cattcctgtt tgccctctga tattgcggat atattaagaa agcataacgc ctgaaatgct
     1141 cactttgcat cagcatagtg atacagctga tgtttattca aaaaccttac tcaagtttta
     1201 agagagcacg gattccctgt cattaataat gatatccgtt agtgtagtct tatgcatt
//
LOCUS       DACADF010000351         1218 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.360, whole genome shotgun sequence.
ACCESSION   DACADF010000351 DACADF010000000
VERSION     DACADF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.360"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(14..1165)
                     /locus_tag="ISB30_004944"
     CDS             complement(14..1165)
                     /locus_tag="ISB30_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HAP1992458.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
BASE COUNT          269 a          287 c          270 g          392 t
ORIGIN      
        1 cgcggttgta gattcaatct gtcaatgcaa cacccctttc aattatctct ttcggtgttt
       61 tgaacttcag tgtctttctc ggtctgttgt ttagctgagc agcaaccaga tctagttcat
      121 gttgagtata ttgggcaaga catgtctttt taggaaagta ctgccgaatt agcccatttg
      181 tgttctcatt tgttccccgc tgccaaggac tctgaggatc gcagaagtaa actttaacgc
      241 cggtgctgac agtaaattct agatgtctgg ccagttccat tcctctgtcc catgtcagtg
      301 attttctgag ttctgacggt aaactcagga atttgtcggt aagagcctga tttactgaga
      361 cagaatcttt gcccctgagt ctaaggatga tcgtataacg tgattttcgg tctacaagtg
      421 tggctatatg agagtttttt gtacctgaga ctaaatcgcc ctcccaatgt cccagagagc
      481 gtctgttatc gatatttcgg gaacgttcgt gaattggtgt tccgttcact atgttaatcg
      541 tacctctttc gcctttgcgg gtatgacgcc tgccatggcg aaggctatgc gaccgtcgca
      601 gatgctgtat attcaggtgg tgtagcgctt cacggctacg aaagtacagc gttttataaa
      661 ttgtctcagg tgatattcgc agcgtttttt gacgtggttt tgttcgcctt aaccatcctg
      721 atatttgctc tggagaccat ttcatctcca gcttttccag aacaagcttt cgcaatggta
      781 aattttgatc cagtaagcac ggttttggcc ttttcgccat tctgttggct cggttattag
      841 catcaacagc tttgtaatag cgtctgcccc gattacgctg aacttcacgt gagatcgtcg
      901 aaggactgcg attcagcgca gtagctatcg cacgaatgct cattttggct gacaaaccag
      961 ctcgtatctc ctcgcgctca gacagtgtca ggtgagctac agcccgctta cgctcatggg
     1021 gttttatgcc gccagtatcc cttaacatag tgaagatcgt tccgggtttt gaacccagga
     1081 tattcgctat ttcactgaag cctgttccgt tcttccatag ttcaaaaaca gaggcttttt
     1141 cctctgctgt aaatgttcgt ctcattcaaa aaacctccgc aaccccatgt tttcacataa
     1201 ctgttgcgtt gaccaatt
//
LOCUS       DACADF010000352         1216 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.361, whole genome shotgun sequence.
ACCESSION   DACADF010000352 DACADF010000000
VERSION     DACADF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.361"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..610
                     /gene="evgA"
                     /locus_tag="ISB30_004945"
     CDS             <1..610
                     /gene="evgA"
                     /locus_tag="ISB30_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAP1992459.1"
                     /translation="AIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETLKP
                     DIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSKKE
                     GMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKDNN
                     DIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            615..>1216
                     /locus_tag="ISB30_004946"
     CDS             615..>1216
                     /locus_tag="ISB30_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP1992460.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASV"
BASE COUNT          383 a          256 c          233 g          344 t
ORIGIN      
        1 cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt tattgatcaa
       61 aaacgatatt gaaatcttag cagagttgac tgaaggcgga agcgccgttc agcgggtgga
      121 aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta acggtatcca
      181 ggtgttagaa acgctgagaa agcgccaata tagcggaatt attattattg tctccgctaa
      241 aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt tcgtgagtaa
      301 aaaagaaggc atgaacaata tcattgcagc tattgaagct gcaaaaaatg gctactgcta
      361 tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc aaaaactcga
      421 ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca aggataataa
      481 tgacattgct gaaaaaatgt tcatcagcaa caaaactgtc agcacttata aaagtcgctt
      541 gatggaaaaa ttagaatgca aatcactgat ggatctttac acattcgcac aacgtaacaa
      601 aatcggctaa ccacatgaag tttttaccct atatttttct tctctgttgt ggtctttggt
      661 cgaccataag tttcgcagac ggagattaca tcgaatatcg tggcatcagt agtaacaacc
      721 gtgtcacact tgatccacta cgtctgagca acaaggaatt acgttggcta gcgagcaaaa
      781 aaaatcttgt gattgcagta cacaagtccc aaacagctac gttgttgcat accgattcgc
      841 agcaacggat tcgtggtatt aatgctgatt atttaaatct tttaaaaaga gcgttaaata
      901 tcaaattaac actccgggaa tacgcagatc atcaaaaagc aatggacgcg ctggaagacg
      961 gtgaagttga tatagtgtta tcacatttag ttgcttcgcc gcctcttaat gatgacattg
     1021 ctgcaactaa tcctctgata attacctttc cggcgctggt cactaccctt cacgattcaa
     1081 tgcgaccgct tacctcatca aaaccagtaa atattgctcg agtagcaaat tatcccccag
     1141 acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca aatttatatc
     1201 aggcattagc atccgt
//
LOCUS       DACADF010000353         1210 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.362, whole genome shotgun sequence.
ACCESSION   DACADF010000353 DACADF010000000
VERSION     DACADF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.362"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>1210
                     /locus_tag="ISB30_004947"
     CDS             <1..>1210
                     /locus_tag="ISB30_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HAP1992461.1"
                     /translation="INGDEATVNNNGNTTVDGQGSTGTEIAGNNVVVNQDGTLDVSGG
                     GHGIDITGDSATVDNKGGMTVTDPDSIGILIDGDKAIVNNDGDNAISNGGTGTQVNGD
                     EATVNNNGNTTVDGQGSTGTEIAGNNAVVNQDGTLDVSGGGHGIDITGDSATVDNKGG
                     MTVTDPDSIGILIDGDKAIVNNDGDNAISNGGTGTQVNGDEATVNNNGKTTVDGQGST
                     GTEIAGNNAVVNQDGTLDVSGGGHGIDITGDSATVDNKGGMTVTDPDSIGILIDGDKA
                     IVNNDGDNAISNGGTGTQINGDDATANNNGKTIVDGKDSTGTEIAGNNAVVNQDGTLD
                     VSGGGHGIDITGDSATVDNAISNGGTGTQVNGDEATVNNNGKTTVDGQGSTGTEIAGN
                     NAVVNQDGTL"
BASE COUNT          331 a          320 c          371 g          188 t
ORIGIN      
        1 agattaatgg cgatgaagcc actgtaaaca ataacggtaa caccaccgtt gatggtcagg
       61 gctctacagg taccgaaatc gcgggtaaca acgtggtggt gaatcaggac ggtacgctgg
      121 atgtcagcgg cggcggtcac ggtattgata tcaccggcga cagcgccacg gtggacaaca
      181 agggcggtat gacggttacc gatccggatt ccatcggtat tctgatcgac ggcgacaaag
      241 ccatcgtcaa caatgacggc gataacgcca tcagcaacgg cggcaccggc acgcaggtta
      301 acggtgatga agccactgta aacaataacg gcaacaccac cgttgatggt cagggctcga
      361 ccggtaccga aatcgcgggt aataacgctg tagtgaatca ggacggcacg ctggatgtca
      421 gcggcggcgg tcacgggatt gatatcaccg gcgacagcgc cacggtggat aacaaaggcg
      481 gtatgacggt taccgatccg gactccatcg gtattctgat cgacggcgac aaagccatcg
      541 tcaacaacga cggcgacaac gccatcagca acggcggcac cggcacgcag gttaacggtg
      601 atgaagccac cgtgaacaat aacggtaaaa ccaccgttga tggtcagggc tcgaccggca
      661 cggaaatcgc gggtaataac gctgtagtga atcaggacgg cacgctggac gtcagcggcg
      721 gcggtcacgg gattgatatc accggcgaca gcgccacggt ggacaacaag ggcggtatga
      781 cggttaccga tccggattcc atcggtattc tgatcgacgg tgataaagcc atcgtcaaca
      841 atgacggcga caacgccatc agcaacggcg gcaccggcac acaaattaac ggtgacgatg
      901 cgaccgcgaa caacaacggc aaaaccattg tcgacggcaa agattccacc ggcacggaaa
      961 tcgcgggtaa taacgctgta gtgaatcagg acggtacgct ggacgtcagc ggcggcggtc
     1021 acggtattga tatcaccggc gacagcgcca cggtggacaa cgccatcagt aacggcggca
     1081 ccggcacgca ggttaacggt gatgaagcca ccgtaaacaa taacggtaaa accaccgttg
     1141 atggtcaggg ctcgaccggc acggaaatcg cgggtaacaa cgcggtggtg aatcaggacg
     1201 gtacgctgga
//
LOCUS       DACADF010000354         1186 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.363, whole genome shotgun sequence.
ACCESSION   DACADF010000354 DACADF010000000
VERSION     DACADF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.363"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            166..1026
                     /locus_tag="ISB30_004948"
     CDS             166..1026
                     /locus_tag="ISB30_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-IId"
                     /protein_id="HAP1992462.1"
                     /translation="MHTRKAITEALQKLGVQTGDLLMVHASLKAIGPVEGGAETVVAA
                     LRSAVGPTGTVMGYASWDRSPYEETLNGARLDDEARRTWLPFDPATAGTYRGFGLLNQ
                     FLVQAPGARRSAHPDASMVAVGPLAETLTEPHELGHALGEGSPVERFVRLGGKALLLG
                     APLNSVTALHYAEAVADIPNKRWVTYEMPMLGRDGEVAWKTASDYDSNGILDCFAIEG
                     KPDAVETIANAYVKLGRHREGVVGFAQCYLFDAQDIVTFGVTYLEKHFGTTPIVPPHE
                     AVERSCEPSG"
     gene            1039..>1186
                     /locus_tag="ISB30_004949"
     CDS             1039..>1186
                     /locus_tag="ISB30_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tunicamycin resistance protein"
                     /protein_id="HAP1992463.1"
                     /translation="MIIWINGPFGAGKTTLAKRLRDRRSKSLIFDPEEIGFVVKETVP
                     MPASG"
BASE COUNT          255 a          314 c          353 g          264 t
ORIGIN      
        1 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
       61 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
      121 cgtcagaccc cgtatagtgt tttgcagttt agaggagata tcgcgatgca tacgcggaag
      181 gcaataacgg aggcgcttca aaaactcgga gtccaaaccg gtgacctctt gatggtgcat
      241 gcctcactta aagcgattgg tccggtcgaa ggaggagcgg agacggtcgt tgccgcgtta
      301 cgctccgcgg ttgggccgac tggcactgtg atgggatacg cgtcgtggga ccgatcaccc
      361 tacgaggaga ctctgaatgg cgctcggctg gatgacgaag cccgccgtac ctggctgccg
      421 ttcgatcccg caacagccgg gacttaccgt gggttcggcc tgctgaatca atttctggtt
      481 caagcccccg gcgcgcggcg cagcgcgcac cccgatgcat cgatggtcgc ggttggtccg
      541 ctggctgaaa cgctgacgga gcctcacgaa ctcggtcacg ccttggggga aggatcgccc
      601 gtcgagcggt tcgttcgcct tggcgggaag gccctgctgt tgggtgcgcc gctaaactcc
      661 gttaccgcat tgcactacgc cgaggcggtt gccgatatcc ccaacaaacg gtgggtgacg
      721 tatgagatgc cgatgcttgg aagagacggt gaagtcgcct ggaaaacggc atcggattac
      781 gattcaaacg gcattctcga ttgctttgct atcgaaggaa agccggatgc ggttgaaact
      841 atagcaaatg cttacgtgaa gctcggtcgc catcgagaag gtgtcgtggg ctttgctcag
      901 tgctacctgt tcgacgcgca ggacatcgtg acgttcggcg tcacctatct tgagaagcat
      961 ttcggaacca ctccgatcgt gcctccgcac gaggccgtcg agcgctcttg cgagccttca
     1021 ggttagaggc cgtcgacaat gataatctgg atcaacggac ctttcggcgc cggaaagacg
     1081 acgctcgcta agcggctgcg cgatcggcgt tccaaatcgc tgatctttga ccccgaggaa
     1141 atcgggttcg tggtgaaaga aacggtcccc atgccagcga gcggag
//
LOCUS       DACADF010000355         1153 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.364, whole genome shotgun sequence.
ACCESSION   DACADF010000355 DACADF010000000
VERSION     DACADF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.364"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..465)
                     /locus_tag="ISB30_004950"
     CDS             complement(<1..465)
                     /locus_tag="ISB30_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAP1992464.1"
                     /translation="MKKNKYSTPLWMLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKETRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIF"
     gene            complement(519..1112)
                     /locus_tag="ISB30_004951"
     CDS             complement(519..1112)
                     /locus_tag="ISB30_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992465.1"
                     /translation="MPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLR
                     IKPNALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVE
                     KPEGVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNG
                     KIACFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
BASE COUNT          348 a          255 c          235 g          315 t
ORIGIN      
        1 aaaaatcgta gcaataaccg caaataatgc accgatgatg cgataagggg taaagtattt
       61 tttctcgtcg acaccgatac caaagcgatc gcaaagtaaa ccagacagca atagcgcgga
      121 gatcagtgcg gtttgaaacg ttgcgacccc gagtgcgctg gctgatgcgc cttcagaaaa
      181 taccaccatc gctccacaga gaccggcaaa ccagttccag agaggaactt ttcggttttt
      241 aatcagagaa ggaattgccg caaattgttg ccgggtttct tttcgcgcca taataataaa
      301 aaacatcacg actaaaccac tggcaaagga gataactgcg catgcattac catcctgtaa
      361 ccagtgacct aattgaccat tcaccgctga ttgcataggg gagagcatac ccgcaagaat
      421 cgtggcaagc atccataagg gagttgagta cttattcttt ttcatacatc aatccttcta
      481 ttatagtaaa tggggtaact ggagttaccc cattattatt atcttgcaat atgagtcagt
      541 atatttaccc acaacgcggt atagaacggc caggacataa actgctgtgt accccagtgc
      601 ggtgaacagt cactcataaa gcaggctatt ttcccgttgt gatagttacc aaagaccagt
      661 aatggatcgt tgtttattgt taataccacc tcagcatttt ctttagcgat cgcccggtta
      721 taacctagga aaaaaggata ttcagagaac cctttaataa ctggatgttc aggttgtgat
      781 ggctgtgcaa taacaccctc tggtttttcg acacgatcgt caccatctaa catcgtaacc
      841 gggagcacat cagccagaac tgtattttta taattggctt ttgcttcaat tcccatgaat
      901 gagagatagc cgccaatcat caataaccca ccaccgttat tcacatattc tttaatcagt
      961 tcgagagcgt ttggttttat gcgaagttga taaaaagtat cattttgtag caggaaagta
     1021 ttactaccaa tatcactaat aacgatggca tcatattgtt ctaactgtgc cacatcttca
     1081 gggaaggcaa tttgtaccgt atgtgccggc atataggtga catctacctg actatttttc
     1141 agacattgga gta
//
LOCUS       DACADF010000356         1132 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.366, whole genome shotgun sequence.
ACCESSION   DACADF010000356 DACADF010000000
VERSION     DACADF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.366"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..105)
                     /locus_tag="ISB30_004952"
     CDS             complement(<1..105)
                     /locus_tag="ISB30_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HAP1992466.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHY"
     gene            complement(326..1057)
                     /locus_tag="ISB30_004953"
     CDS             complement(326..1057)
                     /locus_tag="ISB30_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP1992467.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTRQDTQDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          289 a          268 c          257 g          318 t
ORIGIN      
        1 gtaatgtgca atacggtgat gttttaattc tgaataaaac caggagaagt tttgctcttt
       61 tctcacttgc tcaaacatct tttgaaaaac aacgacctga tccatcagga taataacctc
      121 ttgttaggtg tgagactgcg tagtgtgcac gatcggtttt accacttcaa tctggtctgt
      181 cctttggctg tgatatgtac aaagtgtcaa agggggatta cctgaattct cccggtgagc
      241 attttgcaac ggaccagctc cgctacaaac gctgttgtgg gttcagatta taacattctg
      301 tctaaggggc gggataaagg tgaatttagg gggcatgaaa gatgacttta taaccttgct
      361 caccccagtg ttgtaaaagt tcgttttgcc ttctcgttgg tgccatgcct gtccagacaa
      421 tcaatgtttg cgttgggaac agttccgggc gcggcgattc aatgggatca gcaagaacag
      481 aaatgtgcca tcctgacaat gataaacgcc atgcttccag ccacaatcgt gctctctctt
      541 caacattcca cgccatcaga atggcctctt taccgggctt acggcgcatt tcgaaaagtg
      601 aggccgctgc gtattcaatt aatgcgccgt caaacatact gctcataatg cgggaggtgt
      661 tgtgatcaag cacgagacgc tggcgaacag gaaggtaaac atgattaatc aattgatcta
      721 ctgggtactc tcgacccagt gaaataattc gcgcgcgtag tttggctgga ttagccatac
      781 gaagaattga catcatctct tcttgcaggc ggctccagtc atcttgcgta tcctggcggg
      841 tagtttccag taatgcttta actttgccta caggaacgcc attacttatc caacgcttga
      901 tctcttcgat gcgttgtatg tcttcttcat caaagagtcg gtgtccgcct tcactgcgtt
      961 gtggttttaa caaaccgtag cggcgttgcc aggcccggag agtgacagga ttaatcccgc
     1021 aacgttcagc aacatcacca atgctgtaat aagccacaat tcctccttgc ggtcataaat
     1081 ctccgtcgcc tctacacgac ccaataatac tttgtacaat atacgctaaa at
//
LOCUS       DACADF010000357         1126 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.367, whole genome shotgun sequence.
ACCESSION   DACADF010000357 DACADF010000000
VERSION     DACADF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.367"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            2..955
                     /gene="ompT"
                     /locus_tag="ISB30_004954"
     CDS             2..955
                     /gene="ompT"
                     /locus_tag="ISB30_004954"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HAP1992468.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYIEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          350 a          242 c          246 g          288 t
ORIGIN      
        1 tatgcgggcg aaacttctgg gaatagtcct gacaacccct attgcgatca gctcttttgc
       61 ttctaccgag actttatcgt ttactcctga caacataaat gcggacatta gtcttggaac
      121 tctgagcgga aaaacaaaag agcgtgttta tctggccgaa gaaggaggcc gaaaagtcag
      181 tcaacttgac tggaaattca ataacgctgc aattattaaa ggggcaatta attgggattt
      241 gatgccccag atatctatcg gggctgctgg ctggacaaca ctcggcagcc gaggtggcaa
      301 tatggtcgat caggactgga tggattccag taaccccgga acctggacgg atgaaagtag
      361 acaccctgat acacaactca attatgccaa cgaatttgat ctgaatatca aaggctggct
      421 cctcaacgaa cccaattacc gcctgggact catggccgga tatcaggaaa gccgttatag
      481 ctttacagcc agaggtggtt cctatatcta cagttctgag gagggattca gagatgatat
      541 cggctccttc ccgaatggag aaagagcaat cggctacaaa caacgtttta aaatgcccta
      601 cattggcttg actggaagtt atcgttatga agattttgag ctaggtggca catttaaata
      661 cagcggctgg gtggaagcat ctgataacga tgagcactat gacccaggaa aaagaatcac
      721 ttatcgcagt aaagtcaaag accaaaatta ctattctgtt gcagtcaatg caggttatta
      781 cgtaacacct aacgcaaaag tttatattga aggcgcatgg aatcgggtta cgaataaaaa
      841 aggtaatact tcactttatg atcacaatga taacacttca gactacagca aaaatggtgc
      901 aggcatagaa aactataact tcatcactac tgctggtctt aagtacacct tttaacaacg
      961 ttaaatagat tttctccccg gtggtattgt atagccccgg ggaatttgtt taccgttaaa
     1021 agcaaacaat atcccctaat cttgtaagca tttaacctgc gtcctggcgt gtaacctctt
     1081 accgcaacac aagactcaat atattcaaat tcgttatgcc gtggca
//
LOCUS       DACADF010000358         1125 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.368, whole genome shotgun sequence.
ACCESSION   DACADF010000358 DACADF010000000
VERSION     DACADF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.368"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            14..736
                     /gene="sdiA"
                     /locus_tag="ISB30_004955"
     CDS             14..736
                     /gene="sdiA"
                     /locus_tag="ISB30_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HAP1992469.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(804..1028)
                     /locus_tag="ISB30_004956"
     CDS             complement(804..1028)
                     /locus_tag="ISB30_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAP1992470.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
BASE COUNT          290 a          244 c          273 g          318 t
ORIGIN      
        1 gttgcggttt actatgcagg atacggattt tttcagctgg cttcgcacga tgctgttgcg
       61 ttttcagagg atggaggccg cagaagaggt ctaccatgaa atagagcttc aggctcagca
      121 gctggagtac gattactatt cgttatgtgt tcgccacccg gtaccattca cgcgacctaa
      181 agtggctttc tacaccaatt accctgagtc gtgggttagt tattatcagg caaaaaattt
      241 tctcgcaatt gatccggtgc tgaaccctga aaactttagt cagggccatt taatgtggaa
      301 tgatgactta ttcagcgaag cacagccgtt atgggaagct gcgcgcgcac atggtttacg
      361 ccgcggtgtc actcagtatt taatgctgcc aaatcgggcg ttgggctttt tgtccttttc
      421 ccgttgcagc acgcgcgaaa tacccattct tagtgatgaa ctgcaattaa aaatgcagct
      481 actggtgcgc gaaagtctga tggctctgat gcgtttaaat gatgaaatag tgatgacgcc
      541 agagatgaat ttcagcaagc gcgaaaaaga aattctgaaa tggacggcgg aaggtaagac
      601 atcagcagag atagcgatga ttttgtcaat ctctgagaat acggtcaatt tccatcagaa
      661 aaacatgcag aaaaaaatca atgcaccgaa taagacgcag gttgcctgtt acgcggccgc
      721 tactggctta atttgatctc ttttctgttc tgcgtgccag atgcaaaaac cggctgaaag
      781 gcacgctctc agccggtttt atattactga cggtaggctt gtttaatttg cttaaccgtt
      841 ttggaaaata ctgcagcctg ggtttcgtct tcgatcagcg caagctgttt ttccatcttt
      901 aacatcacgc ggcccgcgtc agcttgtccc atcgcctgca gcatcagcgt cagcatcgct
      961 ttcaggcagg agacttcatt tgccagttct tgattattct cggcagtaga aaaatcaggc
     1021 gtactcattt attttcctcg tcatgttgca atgaaaattt gcggtgaaaa atgttaaggc
     1081 ggcggagtat accataagct ttgctaaaaa tagcagtggt tgttt
//
LOCUS       DACADF010000359         1108 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.370, whole genome shotgun sequence.
ACCESSION   DACADF010000359 DACADF010000000
VERSION     DACADF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.370"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(214..1068)
                     /locus_tag="ISB30_004957"
     CDS             complement(214..1068)
                     /locus_tag="ISB30_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP1992471.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMMEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT          318 a          282 c          239 g          269 t
ORIGIN      
        1 acaaaaaaat caccgacaat aatcacgcat caagtaatag tcgggatttt atttaattga
       61 agcaatttag tttgcatttt tatttaaaaa ctgagctaga gtccatcaaa aaaatatata
      121 aaacccaaaa ccaccgttag aaatagaaat agttgaaagg ctcacaactc ccggaatgcg
      181 gattatcttt gccctgattt ctggcggttt tattcagggt gcaagctgtc tcaccctttc
      241 ccgatattct cccggagtac aaccaaactc gcggacaaac gccttgtgaa aagatgattc
      301 actggcatag cctactgact cagcgatcac cacaacaggg agcatttccc gggaaaacat
      361 ctgggccgct atttgtagac gcaactttgt taatacagcc agcggcgtgg ttccggaaac
      421 atcacggaaa agctgggcaa aacttgcccg ggacatgtgg gcgatgctgg ccagcgattc
      481 gacggtccag gcgtgtccag gcatttccat catttgctgt attaccgcac caagacgtgg
      541 atgcagaagc aaactgagaa tgtttttctc tgtattaacc tgtgcaatcc actcacgcac
      601 cgcgagggta aagaatgtag cgcagatctg gctacacaga gcatccactc ccggcattgc
      661 cgatctggat tcctgttgta aaaacgggat cgcctccctc agccagttat attccacgct
      721 atggttcact ggtgctacaa acagcgtttc cggcaaagac gttaaaaaat aacgcgccga
      781 atgttgcagc cgaagagttc cacagacaat acaggtcgat tcattatcga catgactcag
      841 gcgatgagct gaattttgtg gtagcaggac cacatttccc gggcgtaatg taaaaatctc
      901 tcccgtcggc atttccagct tcgccgctcc ttgcgttaac gcatgccaac gaataaccga
      961 caactccccg gcaccatgtg gaagttgcca gtcacttcct aacacgcaat tcttatcgat
     1021 cgttccttgt ggagcgttaa gcatcaacaa acggctaaga gcatccatat cagactcctg
     1081 agtggttttt ggattacaaa caccaaca
//
LOCUS       DACADF010000360         1098 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.371, whole genome shotgun sequence.
ACCESSION   DACADF010000360 DACADF010000000
VERSION     DACADF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.371"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>1098
                     /locus_tag="ISB30_004958"
     CDS             <1..>1098
                     /locus_tag="ISB30_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HAP1992472.1"
                     /translation="KADSLVGMGLGNNDTGVIAKTDRAISLLRETLAKIIVDLKDAKL
                     VIKRLQFDVFGFSRGAAAARHFTNRVFERDPVLIKTIATAFQSVEYLGKPSGEVQFLG
                     IFDTVAAIGGLMDGFDPHDGNNFPVKVKLPPGVAKHVFQLTAMNECRYNFCLNSIKES
                     WPELSFPGSHADIGGGYDPLEDEYLFLSRPAIETVLGNVPVEATSAYQKAAQQAELLW
                     RHSTLAPILPSGVLKIESNIDEIASPDHLGNVRKRVAAAVTFRRTITNDWSKIALRVM
                     YEVAKEAGVVFDAISVADKELKVPSHLKVICEKAISQGKAVLLCTAPMPFSDDELKTI
                     GKYLHCSSNWNTVDYRLKNKVSAEVSAFKTF"
BASE COUNT          312 a          211 c          273 g          302 t
ORIGIN      
        1 taaagcagac tctctggtag gaatggggct aggaaataat gatactgggg taattgccaa
       61 aactgaccgt gcgatatcac tgcttcgtga gacactggca aagattattg ttgatttaaa
      121 ggatgctaaa ctagtaatta agcgtctaca gtttgacgta tttggcttta gtcgtggggc
      181 ggcagccgca cggcacttta ctaatcgcgt atttgaacgt gatcccgtac tgattaaaac
      241 cattgcgacg gcatttcagt cagttgaata tttaggtaaa ccttccgggg aagtgcaatt
      301 tttaggtatt tttgacacag ttgcagccat cggagggctg atggatggtt ttgaccctca
      361 tgatggtaat aactttccgg taaaagtgaa gttaccgcca ggtgtggcca aacatgtatt
      421 ccagctcaca gcaatgaacg aatgtcgcta taacttttgc ctcaacagca taaaggaaag
      481 ctggccagaa ttaagtttcc ctggatcaca tgctgatatt ggtggcggct atgacccact
      541 ggaagatgag tacctgtttt taagtcgccc ggcgattgaa acggtactgg gaaatgtgcc
      601 ggtggaagcg acaagcgctt accaaaaagc agcacagcag gcagagttat tgtggcgcca
      661 ttcaacgctg gctccgattc tgccttctgg tgtcctaaaa attgaatcca atattgatga
      721 aatagcgtcg cctgaccact taggtaatgt gagaaaacgg gtcgctgcag cggtgacatt
      781 tcgcagaacg atcaccaatg actggtcaaa gattgcgtta agagtaatgt atgaagtcgc
      841 taaagaggca ggggtagtat ttgatgccat ttctgttgct gataaggaat taaaagttcc
      901 cagtcattta aaggtaatat gtgaaaaagc aatttctcag ggtaaagccg ttctcttgtg
      961 tacagcgccg atgccttttt ctgatgatga gcttaaaacc attgggaaat atttacactg
     1021 ctcttccaac tggaataccg tcgattatcg attaaaaaat aaggtgtctg ctgaagtatc
     1081 agcgtttaag acgtttag
//
LOCUS       DACADF010000361         1078 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.372, whole genome shotgun sequence.
ACCESSION   DACADF010000361 DACADF010000000
VERSION     DACADF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.372"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(203..>1078)
                     /locus_tag="ISB30_004959"
     CDS             complement(203..>1078)
                     /locus_tag="ISB30_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1992473.1"
                     /translation="YLLLMAGKVFAFSCNVDGGSSIGAGTTSVYVNLDPVILPGQNLV
                     VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVTYPFPLTTNTNV
                     LDIGDKTPMPLPLKLYITPVGAAGGVVIKSGEVIARIHMYKIATLGSGNPRNFTWNII
                     SNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSARQ
                     VFANTAPDATKASGVGVSLMRNGKTLATGENVSLGTVNKSKVPLGLSATYGQTGNKVA
                     AGAVQSVIGVTFIYE"
BASE COUNT          315 a          233 c          220 g          310 t
ORIGIN      
        1 aaataataga gtagtataaa tacgataagt taagcatcat ctctcatagg gtttaaatta
       61 acataattct caatataaaa aataagactc tggcttcaat tgtgcgcgga ttttcttaca
      121 agtgtaggat aatagccttc acaaaataat tatccgcgca attttcgtga atggagcgca
      181 tagactaatg tcaaaatcac aattattcat agataaaagt gacaccaatg actgactgga
      241 cggctccggc tgcaacctta tttcctgttt gtccataggt tgcgcttaaa cccagaggta
      301 ctttgctttt atttactgtc cccagggaga cattttctcc tgttgccagt gtttttccat
      361 tacgcatcag agagacaccc acgcccgatg cttttgttgc atctggtgct gtgttagcaa
      421 acacttgtcg ggcactgtca gtggtggcac ccgaaagata aaatgagagc ttttgttcgc
      481 tagagcaata tacaccaagg ggtatttctg cgcttccagg aaagtccggg agattcacgg
      541 tgacattacg actatcaacg gtgcaaccgc ctgttggcat aacaacactg ttatttgaaa
      601 taatattcca cgtaaaatta cgtggatttc cgcttcctaa cgtggcaatt ttatacatat
      661 gaatacgggc aataacttcc cctgatttta tgacaactcc accggctgct ccgactgggg
      721 taatatagag ttttaacggt aatggcatcg gtgtcttatc accaatatcc aatacgttgg
      781 tatttgtcgt caacgggaag gggtaggtca cattattcca gtacagtgat cctttatatg
      841 actgtaggga gcctgcgaaa gctgatcctt gtaccaggtt tatatgatca gtgtcgtacc
      901 agccgccgta atcattccag caacttatat gctgagacaa gtctacaacc agattctggc
      961 ccggtagtat tacaggatcg agattaacat aaacagaggt tgtacctgct ccaatacttg
     1021 acccgccatc gacattacaa ctaaaggcaa aaactttgcc tgccattaat agaagata
//
LOCUS       DACADF010000362         1072 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.373, whole genome shotgun sequence.
ACCESSION   DACADF010000362 DACADF010000000
VERSION     DACADF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.373"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..267
                     /locus_tag="ISB30_004960"
     CDS             <1..267
                     /locus_tag="ISB30_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAP1992474.1"
                     /translation="NRDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPV
                     RPAVWLERDDDTGFQQVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            434..817
                     /locus_tag="ISB30_004961"
     CDS             434..817
                     /locus_tag="ISB30_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992475.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYAHYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGEQYCTHCQTGRFSEEGVAE"
     gene            814..999
                     /locus_tag="ISB30_004962"
                     /pseudo
     CDS             814..999
                     /locus_tag="ISB30_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          241 a          285 c          319 g          227 t
ORIGIN      
        1 aaccgggata cctggtgtcg tgccggcgat taccgcgctc cgggatcact ggccgggatg
       61 attaaacagg cgtggtgcag tgccctggga gtggatgccg gacgttatga cacgctggca
      121 cattttccgg tcaggccggc ggtgtggctg gaacgcgatg atgacaccgg ctttcagcag
      181 gtgctggagc gtgctgacta tctggcgaag gagagtacca aagcttacgg caccggtgag
      241 cgcaactttg gctgcagtcg tggctgagcc cgacagacga cttatctgac cttagtgtga
      301 tgacgtgttc acagcgacca cactgatttt cccttctgag actgaactga aaaccgccat
      361 tcacacctga ccacgctgcc cgtaccggga cggctgttgc ctgtgtgtcg tctggcgcag
      421 aggaacatat actatgtacg caaaatcttt tctcgcactt gatggcaacg gacgtctgac
      481 cggtgcccgt accgcacaaa ctgctcctta tgcccactac acctgccacc tttgcggcag
      541 tgcactcaga taccacccgc aatacgacac cgaacggccc tggtttgaac acactgacga
      601 cgggctgacg gagcacgcgc agcaatgtcc ttatgtcagg ccggaacgca gagaaatacg
      661 gttgattaaa cgtctgcagc aattcgtccc ggatgcctta cccgtggtac gtaaagccag
      721 ctggtactgc aggcaatgtc accacgatta ttatggggag cagtactgca cacactgcca
      781 gaccgggcgt ttcagtgagg agggggtagc agaatgaacg gtataaaaac ggggctgggg
      841 atcacgccgg gtgagcacat tatcagtgcc gactctgcgc tcagcagaaa tatccggcat
      901 tgtttctgcc tttcctgtca tggccggttg attctgcaga cagatgctca gggggcgtgg
      961 tttgagcatg atttacatgc cctgagtgag caacagaagg gtaagcgtgc agcaagaacc
     1021 gtattgacgg ggatgtgtta ttcagtcggc agtgctacgc gccaggggag ca
//
LOCUS       DACADF010000363         1065 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.374, whole genome shotgun sequence.
ACCESSION   DACADF010000363 DACADF010000000
VERSION     DACADF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.374"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(440..1012)
                     /gene="hdeD"
                     /locus_tag="ISB30_004963"
     CDS             complement(440..1012)
                     /gene="hdeD"
                     /locus_tag="ISB30_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAP1992476.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
BASE COUNT          368 a          240 c          202 g          255 t
ORIGIN      
        1 attggattgc cagctttctt ttaacgcatt gaaattacct gattaaataa ctcattcatg
       61 tcttcaggtt tttatatagc gtctggagac acggatattt atgcaatgta atcaacgcca
      121 gttgcaaaaa aatgaacccg gattacttat ttaaccgccc atagcatcaa cgcttacgga
      181 actctttgtt cactttccca cagcactttt aaaacattaa acctgaccac ggaaaatatc
      241 agccatgata atgtttgaaa tggctaattt gccatggaac gaaaaaaatc agattaaatt
      301 cagtaggttg aaataatcaa cagcaggcaa ttgtttcaat gatagtgcgt aattgaactg
      361 aaacactgta tagctaagag agtattaact ggatcatagt cacttaatgt gactatgatc
      421 caggtggaaa tagggataat tattgctttt taacgaacaa actggcgaag ctgaacagac
      481 tggcggcgct aaatatcagt tcaattccca ccagtgtgga aaccagcgtt accgacacca
      541 tcggcgtagc accaaggaat atccaggcaa tgacgatatc cagcacacca ataacgagct
      601 gtagccaact gcctttcatt gaacgctgac gataccaact catcaggcga ataacccctg
      661 ccacacagaa caaaccggca ataaatgccg caatggcaaa aatgcccagc tctggtgcgc
      721 ggatgaagaa atagccgatc aataaatagg cgactgcgac gaggaaaccg gataataccg
      781 gccagaaatt atgactgcgg ttgctgaata gcccgacaat aagcgcaatg cccgagcaga
      841 ttaataatgc acccactact gtgcttaaaa tgtcgccaga gacgaacgga aaactgatac
      901 acagcaaccc gacgataaac agcagcacgg caataaactg gattgctctg cgatgttttt
      961 taagcatctc cagatcaaac ttcaaaattg ttgccttatc tatatataac atagaaccac
     1021 cctataaaat taagaagaaa atcccctgct atcaatctat gccaa
//
LOCUS       DACADF010000364         1035 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.375, whole genome shotgun sequence.
ACCESSION   DACADF010000364 DACADF010000000
VERSION     DACADF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.375"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(136..951)
                     /locus_tag="ISB30_004964"
     CDS             complement(136..951)
                     /locus_tag="ISB30_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HAP1992477.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT          306 a          239 c          211 g          279 t
ORIGIN      
        1 tttatgagta aaggattatg tccacgataa gcacctgggt cgattcctgg gaggcggcca
       61 tgagggtagg gaagcgccgt cccgtcaagt cagcgtaatg ctctgccagt gttacaacca
      121 attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
      181 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
      241 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
      301 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
      361 accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac
      421 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
      481 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
      541 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
      601 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt
      661 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
      721 ttccgtcagc cagtttagtc tgaccatttc atctgtaaca tcattggcaa cgctaccttt
      781 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc
      841 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
      901 ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct
      961 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     1021 tgcaatgtaa catca
//
LOCUS       DACADF010000365         1032 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.376, whole genome shotgun sequence.
ACCESSION   DACADF010000365 DACADF010000000
VERSION     DACADF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.376"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>1032
                     /locus_tag="ISB30_004965"
     rRNA            <1..>1032
                     /locus_tag="ISB30_004965"
                     /product="23S ribosomal RNA"
BASE COUNT          291 a          224 c          324 g          193 t
ORIGIN      
        1 cataggttaa tgaggcgaac cgggggaact gaaacatcta agtaccccga ggaaaagaaa
       61 tcaaccgaga ttcccccagt agcggcgagc gaacggggag gagcccagag cctgaatcag
      121 tgtgtgtgtt agtggaagcg tctggaaagg cgcgcgatac agggtgacag ccccgtacac
      181 aaaaatgcac atattgtgag ctcgatgagt agggcgggac acgtggtatc ctgtctgaat
      241 atggggggac catcctccaa ggctaaatac tcctgactga ccgatagtga accagtaccg
      301 tgagggaaag gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga aaccgtgtac
      361 gtacaagcag tgggagcctc ttaatggggt gactgcgtac cttttgtata atgggtcagc
      421 gacttatatt ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa
      481 ctgggcgtta agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg
      541 aaggttgggt aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga
      601 cttgtggctg ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct
      661 atttaggtag cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt
      721 catcccgact taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac
      781 acacggcggg tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt
      841 cccaaagtca tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc
      901 ttagaagcag ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg
      961 gaagatgtaa cggggctaaa ccatgcaccg aagctgcggc agcgacacta tgtgttgttg
     1021 ggtaggggag cg
//
LOCUS       DACADF010000366         1012 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.378, whole genome shotgun sequence.
ACCESSION   DACADF010000366 DACADF010000000
VERSION     DACADF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.378"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..569
                     /locus_tag="ISB30_004966"
     CDS             <1..569
                     /locus_tag="ISB30_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP1992478.1"
                     /translation="LAWGCPTKEHLLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLIS
                     LMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALHGQFDSISMFYLLHCLPGNIS
                     TKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGL
                     THILSEHFENVKTKVQGTVVMFSASGKK"
     gene            complement(624..722)
                     /locus_tag="ISB30_004967"
                     /pseudo
     CDS             complement(624..722)
                     /locus_tag="ISB30_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518755.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(768..950)
                     /locus_tag="ISB30_004968"
     CDS             complement(768..950)
                     /locus_tag="ISB30_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP1992479.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
BASE COUNT          289 a          242 c          201 g          280 t
ORIGIN      
        1 ggcttgcatg gggatgtcct acaaaggaac accttcttcc acactttctg gaacatttag
       61 gtaacaacca tctggatatt ggcgttggaa ctgggtttta ccttactcac gtacctgaga
      121 gtagtctgat atctttaatg gatttgaacg aagctagcct gaacgcggca tctacaaggg
      181 ctggggaatc aaaaattaaa cataaaatta gccatgatgt ttttgaacct tatcccgcgg
      241 cgttacatgg tcaatttgat tccatttcca tgttttacct tcttcactgc ctgcctggaa
      301 atatatctac aaaaagctgt gtaatacgca atgcggcgca ggccttaact gacgatggaa
      361 ctctatacgg agccacaatt cttggcgatg gagttgtgca caatagcttc ggtcaaaaac
      421 tgatgcgcat ttacaatcag aaaggcatct tttcaaacac aaaagattcc gaagaaggct
      481 taacacatat actctcagag catttcgaga atgttaaaac caaggttcaa ggtactgtag
      541 taatgttttc cgcttcaggg aaaaaatagc atccaaccgc agcacgttct tgcttaagac
      601 gtgctgcggc ataatcccaa tgattactcc ctgacagggt tcgtaggcca ctcaatatca
      661 ggtgcagttg atgtatcaac acggttcagt aacacccgat actttttcta ggcttccacc
      721 accagcacga caagatgccg catacagtga accagtcagt ccagttttca gacaaccagt
      781 gcgtcacctt tttgaaggcg ctttaaagca cgttttaatc caggtcggcc tgtccttgtt
      841 ccgcttaatt tatcttcaaa tatttgttca tatcctgcac aaacaagagc gtttcgttgc
      901 agatctgtat tctggtcatt tgttgatacc cttacatagc caatcagcac gctgaatctc
      961 ccgtccaaaa gcacaaatca tgccatgcag gccagaaacc gccattatct aa
//
LOCUS       DACADF010000367         1009 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.379, whole genome shotgun sequence.
ACCESSION   DACADF010000367 DACADF010000000
VERSION     DACADF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.379"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(85..861)
                     /locus_tag="ISB30_004969"
     CDS             complement(85..861)
                     /locus_tag="ISB30_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HAP1992480.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTFS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT          257 a          219 c          236 g          297 t
ORIGIN      
        1 acagtggaaa agtttgataa aatgttcagc ataaaaatga aaattaaatt tcagttaaat
       61 gaacttgaac ggccaattat ccctttactc cttcaactta aaggtaatcg gcatttttac
      121 tttaaataat cctccctcca atatttcagg cggtggtttg ggaagtggtt gagcgcgttc
      181 taaaacagca atagcttcgc ggtcaagaga aaaggttcct gaagagattt ccaagaaact
      241 actgacaata gtgccttccg tgtttacggt aaaagtcacg acggctgtgc cggttcgtgc
      301 acgttttctg gcatcgccag ggtaacgttt ataacggttt aggtggcctt ttactctgct
      361 ttcccaagat gcctcagaat tatctcgctt tgttgagtca ctgttaaaag gcgcagcaat
      421 acgggacgat tcgaccagag cctgaggtgc agcattactg gaaacggctg tggggttgct
      481 gtcgctggtt tgagccttta tcttacgagg tggacgtgtc tttttctttt ctccatcgga
      541 tgactttttc ttcctagtta tttcaatggt tgcatcactg tcttcagtga caggacgttg
      601 ttgtctgtcc tcagtttgtt gcttttcttc tgtcactgcg gactcctgtt gagcgattcc
      661 caccggaagc gaaagtggag aagaaggagc ctctataatt tctgcccatt gcagcatgat
      721 cgccggtgca ggagacatag cgggctgaac ttcggaaaaa cgccagataa acgcaaggaa
      781 aatcagtcca tgaagcagta gagaacagat aaaccagctg ctccatttta ccgactgtga
      841 gaagtggaga atattcatca tccatggttt acctttttgt tgtatctggc gctacaaacc
      901 acacgtagtc agcatgatct actgtgatgt tcattccttt ttctgcaagc atcaacacca
      961 ctggatgggt gccggtggca agatcttcca tgtgtaacgg tacgcccat
//
LOCUS       DACADF010000368          983 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.380, whole genome shotgun sequence.
ACCESSION   DACADF010000368 DACADF010000000
VERSION     DACADF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.380"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..117)
                     /locus_tag="ISB30_004970"
     CDS             complement(<1..117)
                     /locus_tag="ISB30_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992481.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAI"
     gene            complement(117..920)
                     /locus_tag="ISB30_004971"
                     /note="aph(3'')-Ib"
     CDS             complement(117..920)
                     /locus_tag="ISB30_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAP1992482.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT          252 a          278 c          259 g          194 t
ORIGIN      
        1 gattgcagga gtcccgtctg gcaatgaaac tttccaaacg aggctggaaa aggtgtccgc
       61 aatgagaaca ggttgcgaaa cgtgccaatg agcaggaaaa acaggcggca tgaacatcaa
      121 ccccaagtca gagggtccaa tcgcagatag aaggcaaggc gttcgcggtc gggggcttcg
      181 atccccaata cattgaatag gacagcgaag gcgcgctctg cttcatctgg cgctgcccag
      241 ttctcttcgg cgttagcaat catgagtgcc aaatcggcat agcgatctgc tgttccgagc
      301 cgcccaaggt cgatcagacc cgtgcattga agagttttag ggtccaccat gaagttcggc
      361 atgcagggat caccatggca aacaaccata tcggtgcgct cttggtcgag ccgcaccggt
      421 agctctcgtt cgacacgagc caaaagatcg agctgcggcg tactcttgtc ctcgtccggt
      481 aagaagtcgg gattgacggc attgcgggac accacatcaa cggcgcgtcc gaacattcgc
      541 gacagcctgc gctcaaacgg acattgatca accgataggc tgtgaacagc gccaagttgc
      601 tgccccattg acggccacgc tttgagcaaa tccgctccag acagatcagc cgccggtact
      661 cccggaattg ccgttatcac caagcatgca ccctcctgtt cctcctgcca gttgatgacc
      721 tcggggcaag ccacacctcg acctttgagc caaatgaggc ggtcacgctc tccagcgagc
      781 tcaccgcggc gggaagcagg tgcgattttc gcgaaggcat gcccgtcacc acgtcgaaaa
      841 acaaaatcac cagattctcc gcctctgaca ggcaaccagt cagaatgcga ttcaccaaaa
      901 aaaatattag ttcgattcaa tggaggttcc ttcagttttc tgatgaagcg cgaatataga
      961 gaaatatccc gaatgtgcag tta
//
LOCUS       DACADF010000369          969 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.381, whole genome shotgun sequence.
ACCESSION   DACADF010000369 DACADF010000000
VERSION     DACADF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.381"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>969
                     /gene="waaB"
                     /locus_tag="ISB30_004972"
                     /gene_synonym="rfaB"
     CDS             <1..>969
                     /gene="waaB"
                     /locus_tag="ISB30_004972"
                     /gene_synonym="rfaB"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide 1,6-galactosyltransferase"
                     /protein_id="HAP1992483.1"
                     /translation="FIGEAVSGFGGMETVIRDVITTFRQQHIYSEMFFFCRNDKMDKG
                     WLEGIKYSCSFSNIRLGFLRRAKHIHALSKWLQDYQPDVVICIDVISCLYAAKARKKS
                     GIDVPVFSWPHFSLDHKKHAEYITCADYHLAISSGIKQQMINRGVPESTINVIFNPVE
                     AKDTVIPGPEEGETATFIYVGRMKFEGQKRVKDLLDGLSQAQGDWKLHVLGDGSDFEK
                     CQAYGRELKIDNRIVWHGWQKHPWEVVRQDIKKVSALLLTSSFEGFPMTLLESMSWGI
                     PCISADCVSGPGDIIQPDVNGHLYQPGDIAGFVTLLNKYIAGEIHI"
BASE COUNT          271 a          163 c          227 g          308 t
ORIGIN      
        1 catttatcgg cgaagctgtt tctggatttg gcggcatgga aacggtcatt cgtgatgtta
       61 ttactacctt caggcaacaa catatctata gtgaaatgtt tttcttttgt cgtaatgata
      121 aaatggataa gggatggctg gaaggtatta agtactcgtg ttcattttcc aatattcgct
      181 taggtttttt gcgtcgggcg aaacatattc atgctttaag caaatggctt caagattatc
      241 agccagatgt tgttatttgt attgacgtta tctcgtgttt gtatgcggcc aaagcacgta
      301 aaaaatcagg aattgatgtg ccagtatttt catggccgca tttttcttta gatcacaaaa
      361 aacatgctga atatatcacc tgtgcagact atcatttagc catcagctct ggcattaaac
      421 agcaaatgat taatcggggt gtaccagaat cgacgataaa tgttattttt aatccagtcg
      481 aagctaaaga cacagtcatt cctgggcctg aggaaggtga aacggcaacg ttcatttatg
      541 ttgggcgtat gaaattcgag ggccagaaga gggttaaaga cctgcttgat ggtttatcac
      601 aagcgcaagg tgactggaaa ctccatgtac tgggtgatgg ttctgatttt gaaaaatgcc
      661 aggcatatgg tagagaattg aagattgata atcgaattgt ctggcatggt tggcaaaaac
      721 atccatggga ggtagtgcgt caggatatta agaaagtcag tgcattgtta ttaacctcat
      781 cttttgaagg ttttccaatg accttacttg aatcgatgtc atgggggatt ccgtgtatca
      841 gtgccgattg tgtctccggt cctggtgaca ttattcaacc tgatgtcaat ggccatttgt
      901 atcaaccggg agatattgct ggttttgtta ctcttctaaa taaatatata gcaggtgaaa
      961 ttcatatcg
//
LOCUS       DACADF010000370          943 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.383, whole genome shotgun sequence.
ACCESSION   DACADF010000370 DACADF010000000
VERSION     DACADF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.383"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            100..>943
                     /gene="tdcA"
                     /locus_tag="ISB30_004973"
     CDS             100..>943
                     /gene="tdcA"
                     /locus_tag="ISB30_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAP1992484.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTATLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNY"
BASE COUNT          257 a          183 c          217 g          286 t
ORIGIN      
        1 gaaagtattg aaagttaatt tgtgagtggt cgcacatatc ctgttcattt cattttgata
       61 cacttcatac cgtcaatgag gtaattaacg taggtcgtta tgagcactat tcttcttccg
      121 aaaacgcagc acctggtagt ctttcaggaa gttattagaa gtggttctat cggctcggct
      181 gcaaaagagt tagggttaac tcaaccggcc gtcagtaaaa tcattaacga tattgaagat
      241 tattttgggg tggagttagt ggtgcggaaa aataccggtg taacattaac acctgccggt
      301 caattgttac tctcccgttc cgaatccatt acccgtgaaa tgaaaaatat ggttaatgag
      361 ataagcggta tgtcttctga ggcggtggtg gaagtctcat ttggttttcc ttcattgatt
      421 ggttttactt ttatgtcagg gatgatcaac aagttcaaag aggtgttccc gaaagcgcag
      481 gtttctatgt atgaagcgca actgtcttcg ttcttaccgg caatccgcga cggtcgactg
      541 gattttgcga ttggtacgtt aagcgcagaa atgaagcttc aggatttaca tgttgagccg
      601 ctgttcgagt ccgagtttgt gctggtagcc agtaagtccc gaacatgcac cggcaccgcc
      661 acgctggagt cgttgaagaa cgaacagtgg gtgttgccac aaaccaatat ggggtactac
      721 agcgaactac ttactacgtt acaaagaaat ggcatcagta ttgaaaacat cgttaaaacc
      781 gactcagtcg tgacaattta taatcttgtt ctcaatgctg atttcttaac tgtaattcct
      841 tgtgatatga catcaccttt tggttctaat caatttatta ctattccggt tgaagaaaca
      901 ttacctgtgg cacaatatgc cgcggtatgg tcgaaaaatt atc
//
LOCUS       DACADF010000371          925 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.384, whole genome shotgun sequence.
ACCESSION   DACADF010000371 DACADF010000000
VERSION     DACADF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.384"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>925
                     /gene="evgS"
                     /locus_tag="ISB30_004974"
     CDS             <1..>925
                     /gene="evgS"
                     /locus_tag="ISB30_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAP1992485.1"
                     /translation="SQNWLDTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPY
                     SMTDETGSVRGVMGDILNIITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSE
                     DRENNVSFAEVFITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEW
                     IKVDNASAAFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFP
                     RGEPELKDIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLV
                     GSSLLWGFYLLRSVRRRKVIQGDLENQISFRK"
BASE COUNT          280 a          192 c          185 g          268 t
ORIGIN      
        1 tcacaaaatt ggcttgatac aggaaacctg gcctttctga acaaaccatt agaactcact
       61 gaacatgaaa aacagtggat taagcagcat cccgatttaa aggtgctgga aaatccttac
      121 tcgccaccct attctatgac agatgaaact ggctcagttc ggggcgtgat gggtgatatt
      181 cttaatatta ttaccttgca aacaggttta aatttttctc cgatcaccgt ttcacacaat
      241 attcatgctg ggacacagct taaccccggt ggatgggata tattgcccgc cgctatttat
      301 agtgaagatc gagaaaataa tgtttcattt gctgaagtct tcataacaac gccttacgtt
      361 tttgtcatgc aaaaagcgcc tgacagtgaa caaacattaa aaaaaggaat gaaagttgcc
      421 attccatatt attatgagct tcattcgcaa ttaaaagaga tgtatccgga ggtagagtgg
      481 ataaaagtcg ataacgccag cgctgcattt cacaaggtca aggaaggtga acttgatgct
      541 ctggtcgcga cacagttaaa ttcgcgttac atgatcgacc attactatcc taatgaactt
      601 tatcattttc ttattcccgg cgttcagaat gcatcacttt cgttcgcttt tcctcgcgga
      661 gaaccggaac ttaaggatat tattaataaa gcactgaatg caattccccc aagcgaagtt
      721 ctgcgcctga ccgaaaaatg gattaaaatg cccaatgtga ccattgacac atgggacctc
      781 tatagcgagc aattttatat tgttacgaca ttatccgttt tattagttgg cagtagcctt
      841 ttatggggat tctacctgtt acgctcagtt cgtcgtcgta aagttattca gggtgattta
      901 gaaaaccaaa tatcattccg gaaag
//
LOCUS       DACADF010000372          923 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.385, whole genome shotgun sequence.
ACCESSION   DACADF010000372 DACADF010000000
VERSION     DACADF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.385"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(554..729)
                     /locus_tag="ISB30_004975"
                     /pseudo
     CDS             complement(554..729)
                     /locus_tag="ISB30_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          287 a          188 c          191 g          257 t
ORIGIN      
        1 caaaatacat agctacatta ttaaaacaag tgaatatccg gaggagtttt tttccaatgc
       61 agtatggggt aaaaaaaata taaaaccggg ggaatattac tctgtgcagg ctgtttctat
      121 gtttacgaca aaccagttta ttctcctttg tcccaaggat attataaggt tttatcggga
      181 aagctggaag ttcgacctgc ttaatgtaga cttcgataca aataccagga agaaactcca
      241 ggaagaagca gcatattgcg taccaatttc tgcaatatct gtaaaacgat gggatatgcc
      301 tcttcagggc tcgaaaccct caaattagaa ggccgctcgt gcgactgtat tagatagtaa
      361 tgcggtatgg aaaaatagtg atggcaagat gaacttgact tggctaatca aataccggcg
      421 tagtgattag gaaacagggc tcggataaca ttattgcgcg gttcattgaa cctactgatg
      481 acgccggata tatagtcgta aaccgtttcg aaccagtact tatatcacca ggagagtcca
      541 tatatgacca ccgtttacag taaaaacggt atctgttttc tgatccctcc agcgtcaaac
      601 taccgttgta atggtgaggt ctggtgctat tgtaataatt cagaatataa tctctgattt
      661 atttccgaac ctcgcccttg cctgcgtaac catccgtcgc ccccatcctg ttttcagact
      721 gcggaaaaag gcaagttttt atccccccac agagattcaa tcatatatcg cgtgttttgg
      781 aagtgacagt tcttattcct gagaatactg catgtgcaac acactcggca tccatcgata
      841 ccacttttat ctccaatagg caggataaaa aacaagactg tgaattaaag taatcggcaa
      901 atgaatgagt tagaagatac tga
//
LOCUS       DACADF010000373          911 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.386, whole genome shotgun sequence.
ACCESSION   DACADF010000373 DACADF010000000
VERSION     DACADF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.386"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(136..768)
                     /locus_tag="ISB30_004976"
     CDS             complement(136..768)
                     /locus_tag="ISB30_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAP1992486.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          223 a          213 c          188 g          287 t
ORIGIN      
        1 aggaagataa aatgcgagta tatagaaccg cgagcgccaa ataattttat gaccacaacc
       61 tgtcgtattg tggtcataaa aaattgtcgg ctggagggag ttaaatcctt tacctcaata
      121 tattgcctgt caggtttagt tcacaaacag tgcttcgcta tcaacgatga ttccatcttc
      181 cgcaccttta agcgccaggt ttgcctcgta gtattgcttt ttatcaagca gtttctgcgc
      241 atcagcaagt gcattacgag tctgtttgag cggcatcaga tattggtttt ccattacgcc
      301 aacaccggcc aggcgcagct cttccattgc gcccttttta tcccctttcg ccattttttc
      361 gttggcaatt tttatcgcgg cttctttttc gggtgtcgct acataactct cagaaattcc
      421 gacagatgca ttaatgacaa tatattggtc atcattgacg ttggtctttt tacctggctt
      481 agcgaacttt gcccattcgg tactatcatc tgacagcaaa gcggaagctt cattggtcag
      541 ttcttttgcc ttttctggat cgccatgaaa cagagccagg cgcgctacct gcacgtcacg
      601 catcgcatat aaaccttgtt cagagatacg ctctgctgct gccgttttct gggtttgctt
      661 aacttgctga gtttgctgtt gtgccgctac cggtgcatta tcagcagccc atacagttga
      721 agatgccaga attgctgtga ccatcgtagc cataattaaa cgtttcatac ttacctcatt
      781 ctttaacagt tttagaaaac gccgccatca tattgggtga gcatgaaatg agaaatcaga
      841 gagcaacggt cattttgaaa gggttatctt acaactgtag ttattatcgc aaagccgctt
      901 ttagtcttac a
//
LOCUS       DACADF010000374          899 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.387, whole genome shotgun sequence.
ACCESSION   DACADF010000374 DACADF010000000
VERSION     DACADF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.387"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..313)
                     /locus_tag="ISB30_004977"
     CDS             complement(<1..313)
                     /locus_tag="ISB30_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992487.1"
                     /translation="MLSWLYDGRVKRRPLMNWLIQTYQQRWPLHEWLTEGIEEDRLDW
                     LIAQVLQKGHYRRQFPVEITRPFAGKRGLTDGRLFREMQRFLDVTDHSRLIMLSDQFH
                     WS"
     gene            complement(433..825)
                     /locus_tag="ISB30_004978"
     CDS             complement(433..825)
                     /locus_tag="ISB30_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2787 domain-containing protein"
                     /protein_id="HAP1992488.1"
                     /translation="MPVDQELINIILNEAGNPPPHKAKITAVSLLFKDLSYSAEKGGY
                     HPVEIRIISRNDEWYFDYITDFSYMGTVYPELEKEIDISWSQQYVFLAYVGDLPVNAG
                     RELFELWQSNFIQYHGMNIYTITVLWES"
BASE COUNT          236 a          221 c          196 g          246 t
ORIGIN      
        1 gggaccagtg aaactggtcg ctgagcataa tcaggcgaga gtggtcagtg acgtcaagaa
       61 accgctgcat ttccctgaac agccgcccgt ctgttaaccc ccgcttccct gcaaagggtc
      121 tggtgatttc aaccgggaac tggcggcggt aatgcccttt ctgcaacacc tgagctatta
      181 accagtctaa gcggtcttct tcaattcctt cagtcagcca ttcatgcaaa ggccagcgct
      241 gctgataagt ctgaataagc cagttcatta acgggcgtcg tttcaccctg ccgtcataca
      301 gccagctcag catgttaacc acgcaataag caccgcaaag acagtccagt tcgccctgtt
      361 gagacgcacg tatccctctg tcagtcgtca cagataattc cccataaatt agtgatggtt
      421 agtacagggg gattagcttt cccagagaac ggtaatggtg tagatgttca tgccgtgata
      481 ctgaataaag ttggattgcc agagttcaaa cagctccctt ccggcgttaa caggcaggtc
      541 accaacataa gcaagaaaga catattgctg actccagctg atatcaattt ccttttcaag
      601 ttcggggtat accgtcccca tataactgaa gtcggtaatg taatcaaaat accattcatc
      661 attgcgtgaa ataatccgta tttcgacagg atggtaacca cccttttcag cgctgtaact
      721 caggtcttta aataacagac tgacggctgt aattttggct ttatgcggcg gtggatttcc
      781 cgcttcattt aatatgatat ttattaattc ctgatcgacg ggcatagcta gcccttcctg
      841 atggacaata ttcatcagtg attttcctgt tcaatatatt ctgataagac gttatgtct
//
LOCUS       DACADF010000375          892 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.388, whole genome shotgun sequence.
ACCESSION   DACADF010000375 DACADF010000000
VERSION     DACADF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.388"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(1..345)
                     /locus_tag="ISB30_004979"
                     /pseudo
     CDS             complement(1..345)
                     /locus_tag="ISB30_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(394..741)
                     /gene="tnpB"
                     /locus_tag="ISB30_004980"
     CDS             complement(394..741)
                     /gene="tnpB"
                     /locus_tag="ISB30_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP1992489.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          181 a          240 c          255 g          216 t
ORIGIN      
        1 cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt ctgacgcaac
       61 ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc tatcgctctc
      121 tttctgaagc cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga
      181 acgactgccg aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc
      241 gcgttcacgc tcgttcagct tttccagcag ggcacggttc agcgcctcct gttcggcaag
      301 gagacgtttc agtgcattga tatcgtcagg aagtgagctg ctcataccgg gtatattacc
      361 aggctcattc agcgtcgacc aggataaaga ggcttacaac atagtcaggg acgtaagcag
      421 tcttttaggc tgccgccagt cgataccttc aaggagcatc gccagctgtg ccggtgtgag
      481 gaacactttg ccatcccggg ctgacggcca ggcgaagcgg ccgcgctcca gccgtttggt
      541 cagcagacac agtccatcgc cggtagacca gaggagcttt acctgactgc cattacgccc
      601 acggaagata aaaacgtgac ctgacatcgg atcgtctttc agcgtcgtct gcacctttgc
      661 cgccaggccg ttgaagccgt ttctcatatc ggtgatgcca gcgaccagcc agatctttgt
      721 ccctgatggt aatgggatca acgtttgagc tcccgtatca gcaggttcag gatattttcg
      781 ctgatggcac cattcagacg gagtgatccg tgccggaacg ttacttcaca gctgatattg
      841 agtgattcag gtgtcgcagc agctacaggt tgtgaccggg gggaggttgt ag
//
LOCUS       DACADF010000376          876 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.389, whole genome shotgun sequence.
ACCESSION   DACADF010000376 DACADF010000000
VERSION     DACADF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.389"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            6..566
                     /gene="slp"
                     /locus_tag="ISB30_004981"
     CDS             6..566
                     /gene="slp"
                     /locus_tag="ISB30_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAP1992490.1"
                     /translation="MTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQPG
                     LYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFLDP
                     VNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYGYG
                     AFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            719..>876
                     /locus_tag="ISB30_004982"
     CDS             719..>876
                     /locus_tag="ISB30_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP1992491.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNV"
BASE COUNT          257 a          184 c          187 g          248 t
ORIGIN      
        1 tgaacatgac aaaaggtgca ctcatcctca gcctttcatt tttgcttgcc gcatgtagtt
       61 caattccgca aaatatcaaa ggcaataacc aacctgatat tcaaaaaagt tttgttgctg
      121 ttcataacca gccggggtta tatgttggtc aacaagcgcg ctttggtggg aaggttatca
      181 acgttatcaa tggcaaaacg gatacgttgt tagaaatcgc tgtattaccg ttggatagct
      241 atgcgaagcc tgatattgaa gccaactatc agggccgact gctcgccaga caaagcggct
      301 tccttgatcc agtgaactat cgtaatcact ttgttaccat cctcggtacc attcagggtg
      361 aacaacctgg ctttatcaat aaagtcccgt ataacttcct cgaagtgaat atgcagggca
      421 tccaggtgtg gcatttgaga gaagtggtta ataccaccta taacctgtgg gattacggct
      481 atggtgcatt ctggccggaa ccgggctggg gggctcctta ctacaccaat gcggtgagtc
      541 aggtaacacc tgagctggtc aaataacacc accgaaagat gcagatgctt tctcagcatc
      601 tgcatcatgc attacatcaa attaatacac agtaagctaa ctattattat aagccctgtc
      661 ctgttaatta cctttggcaa actgattata aagttaatgt ccgtaccagg agtcggttat
      721 gtttcttata attaccaggg atacgatgtt tttcaccgcg atgaaaaaca ttctgagtaa
      781 aggtaatgtc gttcatatac agaacgaaga agagatcgac gtaatgttgc atcagaatgc
      841 cttcgtcatt attgatacat taatgaataa tgtatt
//
LOCUS       DACADF010000377          868 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.390, whole genome shotgun sequence.
ACCESSION   DACADF010000377 DACADF010000000
VERSION     DACADF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.390"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..773)
                     /locus_tag="ISB30_004983"
     CDS             complement(<1..773)
                     /locus_tag="ISB30_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HAP1992492.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSPVVEQNLMPWLATGAATALITALAIVELNDDDDHHHRNNSP
                     LPPTPPDDNSDDTPVPPTPGGDEIIPDDGSDDTPTPPKPISFNNDVILDKTAKTLTIR
                     DSVFTYTENADGTISLQDSNGRKATINIWQIDEANNTVALDGVSADGATKWQYNHNGE
                     LVITGDNATVNNNGKTIVDGKDSTGTEIAGNNGKVIQDG"
BASE COUNT          218 a          191 c          196 g          263 t
ORIGIN      
        1 ataccgtcct gaataacttt cccgttatta ccggcgatct ccgtgccagt ggaatcttta
       61 ccatcaacga tggttttgcc attgttgttt actgtggcat tatcacccgt aataacaagc
      121 tcaccgttat gattatattg ccacttcgtt gcgccatctg cgctcacccc gtcaagggca
      181 accgtgttat tcgcttcatc aatttgccaa atattaatag ttgccttacg gccattgcta
      241 tcttgtagag aaatagtccc gtcagcattc tcggtataag taaaaactga atcgcgaata
      301 gttaacgttt ttgctgtttt atcgaggata acgtcattat taaacgaaat tggtttggga
      361 ggcgtcgggg tatcatccga accgtcgtct ggtattatct catctccgcc aggagttggg
      421 ggaactggag tgtcgtctga attatcatca ggtggagttg gtggaagtgg agaattattg
      481 cgatgatgat gatcatcatc gtcgtttagt tccactatgg ctaaggctgt gattaacgca
      541 gtagcagcac ctgtggcaag ccatggcatc aggttctgtt cgactacagg ggagcattca
      601 gagggtaagc gtttaagctc ttctggactg agttttccag ggtctgccgg acaagtcaca
      661 ttggtatttt tcttttcacc tgtggtggtt tctatctctg tgatatctgc cgcccagccc
      721 tgaccactca gagccaatgc aatacaggct gacaatagag ttttcctttg cataataaat
      781 ccttctggcg aatataaaga aggtcggatg ccttaaccca accaattaat caatcaatta
      841 aaacatagag gacattcaat attgccag
//
LOCUS       DACADF010000378          842 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.395, whole genome shotgun sequence.
ACCESSION   DACADF010000378 DACADF010000000
VERSION     DACADF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.395"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>842
                     /locus_tag="ISB30_004984"
     CDS             <1..>842
                     /locus_tag="ISB30_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1992493.1"
                     /translation="AVSELASRVSSKTGRKLLAIFIVSALSYGAAEYACAAQMDTRNF
                     WIRDYLDLAQNKGAFQPGAYGVKTPLKNGGEFSFPEVTIPDFSPVSAKGATTAIGNAY
                     SVTASHNGTIHHAIKTQTWGQSDYHYVDRVTKGDFAVQRLDKFVVETAGATEHADFNL
                     SAAEALERYGIEFNGKKQIIGFRVGAGATGVTSYGVGQTYNPLLRSASMFQLNWNNMY
                     ASNNTGGFYNEVTGGDSGSGFYLYDNQKKKWVILGTLTGKVFSSKDTWAFFARYDQNT
                     VDIL"
BASE COUNT          245 a          161 c          197 g          239 t
ORIGIN      
        1 gctgtatcag aattagcatc ccgagtatcc tctaaaaccg gtcgaaaact tctcgctata
       61 tttatagttt ctgccttgtc ttatggtgcg gctgaatatg cctgtgcagc acagatggac
      121 acccgaaatt tctggatccg tgattatctt gatctggcac agaataaagg tgcatttcag
      181 cccggagcat acggggtaaa aacgccatta aaaaatgggg gagaattcag ttttcctgaa
      241 gtaacaatcc ctgatttttc tcctgtatcc gctaaaggtg caacaactgc tattggtaat
      301 gcctacagtg ttacagcaag tcataatggc actattcacc atgccattaa aactcagaca
      361 tggggacagt cagattatca ttatgttgat cgtgtgacca aaggtgactt tgcggtccag
      421 cgtctggata agtttgttgt tgaaacagcg ggtgcaacag agcatgctga tttcaactta
      481 tcagcagcag aagcactaga gcgttatggt attgaattca atggaaagaa acaaataatc
      541 ggtttccgcg ttggagctgg tgcaaccggt gttacatctt atggggtggg acagacatac
      601 aatccattat tacgtagtgc ctccatgttt cagttaaact ggaacaatat gtatgcaagt
      661 aataataccg gtgggtttta taatgaagtg acaggaggtg acagcggttc cggattttac
      721 ttgtatgata atcagaaaaa aaaatgggtc attttgggga cactgacagg taaagtattc
      781 tccagtaagg atacctgggc cttttttgct cgatatgatc agaacactgt cgatatcctg
      841 aa
//
LOCUS       DACADF010000379          836 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.396, whole genome shotgun sequence.
ACCESSION   DACADF010000379 DACADF010000000
VERSION     DACADF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.396"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..707
                     /gene="ydeO"
                     /locus_tag="ISB30_004985"
     CDS             <1..707
                     /gene="ydeO"
                     /locus_tag="ISB30_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAP1992494.1"
                     /translation="DKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKYLQ
                     CTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAAC
                     KGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFS
                     QILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQ
                     RHTGMNTDNTMNALAS"
BASE COUNT          259 a          169 c          169 g          239 t
ORIGIN      
        1 tagataaaga ttacgccttc tctgacggcg agatcctgat ggtagataac gctgttcgca
       61 cgcattttga accttatgag cggcatttca aagaaatcgg atttaatgaa aataccatta
      121 aaaaatatct acaatgcact aacatccaga cagtgacgat gcctgttcct gcgaagtttt
      181 tacgtgcttc aaatgtaccg actggattgc ttaatgaaat gattgcttat ctcaactcgg
      241 aagaacgcaa tcatcataat ttttcagagc ttttgctttt ttcttgcctg tctatttttg
      301 ccgcatgcaa aggtttcatt acactattaa ctaacggtgt gctatccgtt tccgggaaag
      361 taagaaatat tgtcaacatg aagctggcgc acccatggaa gctgaaagat atttgtgact
      421 gcctgtacat cagtgaaagc ctgttgaaga aaaaactaaa gcaagagcaa acgacattct
      481 cacagattct tttagatgcc agaatgcagc acgcgaaaaa tttgatacgc gtagaaggtt
      541 cagtcaataa aattgctgaa caatgcggtt atgccagtac atcttatttt atttatgcgt
      601 ttcgcaaaca tttcggcaac agtccgaaga gagtttctaa ggagtaccgt tgtcaaaggc
      661 acacgggtat gaacacggac aacacgatga atgctttagc tagttgatta tttgctaacg
      721 agtagtcaac cacacacgct gcgtaagaat taaatggggc agccattccc tgccccgcgt
      781 tgtttccagg cgatatattt cttgaaataa ataagtgaca gccatcacat atttat
//
LOCUS       DACADF010000380          823 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.397, whole genome shotgun sequence.
ACCESSION   DACADF010000380 DACADF010000000
VERSION     DACADF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.397"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            700..>823
                     /locus_tag="ISB30_004986"
     CDS             700..>823
                     /locus_tag="ISB30_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003840849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HAP1992495.1"
                     /translation="MGWTEAADLIVKGMEGAINAKTVTYDFERLMEGAKLLKCSE"
BASE COUNT          283 a          139 c          184 g          217 t
ORIGIN      
        1 aaaggcgtga ttcaatgcat tggttacatt gacaaaaata acaaagcaag ttgcgatgaa
       61 ttttattcag gcagctatga atgcttggat gatgatcagg aaacactgaa ctatttaaat
      121 gaatcatgcg cagagggtat agacgctgat aaagatctca tcaggaagaa tgagttatta
      181 gccttatccc tgaaggaatt gtatcaactt ctcgtttcaa aaaaataaca agtaatacgg
      241 cgagcgatat gacgtgccat gggatatccg cacccagcag catgttgtct ccacgatgtc
      301 cgcagttcgc tcataataga cattcactac agttatagta gaaggtatgc atgctgctga
      361 gtggggaaag tgtgaaagaa aagaagacag ctgctttgtt tgtcgtcacg tttatcttca
      421 ttggctatgc aagtcgtaat acaaggcggg acaaaactga gacacataag gcctcgcaat
      481 ggcttgcaag gccacacatg tattgagatg ttaataaaat gtagacttgt aattttgata
      541 taaatggtag agaaaatctt tccccaaaat aaaaacgaac gtcaatgaaa tcaaacggtt
      601 gaataaagtt gattttggct aatacaaaga caagaaaata atatttatga ttaaatatca
      661 gcgagttgaa tacataattt ttatatactg ctgcgccaca tgggttggac tgaagccgca
      721 gacctgattg ttaaaggtat ggaaggcgca atcaatgcga agaccgtaac ctatgacttc
      781 gaacgtctga tggaaggcgc taaactgctg aaatgttcag agt
//
LOCUS       DACADF010000381          821 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.398, whole genome shotgun sequence.
ACCESSION   DACADF010000381 DACADF010000000
VERSION     DACADF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.398"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>821
                     /locus_tag="ISB30_004987"
     CDS             <1..>821
                     /locus_tag="ISB30_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP1992496.1"
                     /translation="AVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGALRLL
                     VSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSVINDA
                     QAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSGVTDT
                     ECGCGRRGCVEAVSSGRAIMGAAKNKLAGYSTKYIFELARQGYKEAEFLTERSASTIA
                     ELIVSLKLLLDCQVVVVGGSVGLADGYVQKVSKHLSIYSEICNVMLFPAYFRSDSG"
BASE COUNT          246 a          116 c          218 g          241 t
ORIGIN      
        1 gctgttgata taggaggaac gaaaatttca gcggcgttga tatctgatga cggttcattt
       61 ttgctgaaaa aacagatatc aacacctcat gaaagatgtc ctgacgaaat gacaggggct
      121 ttacgtctac tggtttctga aatgaaggga acagcggagc gatttgcagt agcatcaaca
      181 gggattatta ataatggagt attgacagct ctgaatccag acaatcttgg agggctgaaa
      241 gagtatcctc tgaaaaatat aatggaagat ataactggtt taaatggaag tgtgataaat
      301 gatgcccagg cagccgcatg ggcagaatat acagttttgc cgaaagaaat ttgtgatatg
      361 gtttttataa ccgtttctac gggggttgga gggggaattg tagttaacag aaaattatta
      421 acaggtgttt ctggattagc cggacatgtt gggcatatat tgtccggagt aacggataca
      481 gaatgtggat gtggtcgtcg gggatgcgtt gaagcggttt catctggtcg ggccatcatg
      541 ggggcagcaa aaaataaact tgcaggatat agcaccaaat atatttttga attagcgaga
      601 cagggatata aggaagcaga atttttgaca gagcgttctg ccagtactat tgctgaatta
      661 atagtaagtt taaaattact gcttgattgt caggttgttg ttgtcggagg aagtgttgga
      721 cttgctgatg ggtatgttca gaaagtgtcg aaacatcttt ccatatattc tgaaatttgt
      781 aatgtaatgc tgtttcctgc atatttcaga agtgactctg g
//
LOCUS       DACADF010000382          779 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.399, whole genome shotgun sequence.
ACCESSION   DACADF010000382 DACADF010000000
VERSION     DACADF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.399"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            119..>779
                     /gene="intI1"
                     /locus_tag="ISB30_004988"
     CDS             119..>779
                     /gene="intI1"
                     /locus_tag="ISB30_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAP1992497.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KY"
BASE COUNT          147 a          223 c          240 g          169 t
ORIGIN      
        1 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
       61 cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat
      121 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
      181 tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg tccactgggt
      241 tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt
      301 cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca
      361 ggcattggcg gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct
      421 tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga
      481 agtggttcgc atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc agcttctgta
      541 tggaacgggc atgcggatca gtgagggttt gcaactgcgg gtcaaggatc tggatttcga
      601 tcacggcacg atcatcgtgc gggagggcaa gggctccaag gatcgggcct tgatgttacc
      661 cgagagcttg gcacccagcc tgcgcgagca gctgtcgcgt gcacgggcat ggtggctgaa
      721 ggaccaggcc gagggccgca gcggcgttgc gcttcccgac gcccttgagc ggaagtatc
//
LOCUS       DACADF010000383          772 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.400, whole genome shotgun sequence.
ACCESSION   DACADF010000383 DACADF010000000
VERSION     DACADF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.400"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..235)
                     /locus_tag="ISB30_004989"
     CDS             complement(<1..235)
                     /locus_tag="ISB30_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP1992498.1"
                     /translation="MSVFSRVCLGFTFLLTALQVQASIVLGGTRIIYPSNQNEVQITL
                     KNKDAYARYLVQSWVSNIDGSKAPFLITPPVYKL"
     gene            complement(281..>772)
                     /locus_tag="ISB30_004990"
     CDS             complement(281..>772)
                     /locus_tag="ISB30_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAP1992499.1"
                     /translation="DTGVVNFNGKIVADTCEINVNESASTTGTVTFADTYPADYSGDG
                     SIGTSKDFKIELIKCDPLVDKLNLTFSGTTTDAGFLRLENSEAGAGSATNVGITVTNK
                     NGGANDVKFDGSVPDASTDVDNAGSITPTIFNYTANVIQVGNNAPTAGKYASSATFEV
                     FYR"
BASE COUNT          233 a          174 c          142 g          223 t
ORIGIN      
        1 caagtttata aactggaggc gtaatgagaa agggggcttt agagccatca atattactca
       61 cccagctttg caccaaatag cgtgcatatg catctttatt ttttaatgtg atttgaactt
      121 cattttgatt tgaaggatag ataatacgtg taccgccaag aacaatactt gcctgaactt
      181 gcaatgccgt aagaagaaac gtaaaaccta aacaaactcg agaaaaaact gacataaata
      241 accctccgtg gtcaaacagt cctttcaaac actatgaata ttagcgataa aatacttcga
      301 acgtcgcaga tgaggcatac ttacccgcag tcggcgcgtt attaccaacc tgaataacat
      361 tggcggtgta attgaatatt gttggagtaa tgctcccggc attatcaaca tcagtggatg
      421 catcgggaac tgaaccatca aatttcacat cgttagcacc gccattttta tttgtcaccg
      481 taatacccac atttgttgca ctgccagccc cagcttcgct attttccaga cgcaggaaac
      541 ctgcatccgt tgtcgttcca gaaaatgtca ggtttagttt gtccaccaac ggatcgcatt
      601 ttattaattc aattttaaaa tcttttgacg ttccaatact accgtctcca gaatagtccg
      661 ctgggtaagt atctgcaaaa gtgactgtcc ctgttgtact ggcagactcg tttacattaa
      721 tttcgcaagt atcggcaaca attttaccgt taaagttaac cactccggta tc
//
LOCUS       DACADF010000384          755 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.401, whole genome shotgun sequence.
ACCESSION   DACADF010000384 DACADF010000000
VERSION     DACADF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.401"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>755
                     /gene="vgrG"
                     /locus_tag="ISB30_004991"
     CDS             <1..>755
                     /gene="vgrG"
                     /locus_tag="ISB30_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAP1992500.1"
                     /translation="ARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFDGETEAVWGLNT
                     AYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADNFLQKGDKEAAE
                     SGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAVFRKGVLITGVTAS
                     AARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKNDIYAHIDKDGRY
                     RVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGL"
BASE COUNT          177 a          227 c          227 g          124 t
ORIGIN      
        1 acgcccggct gaaaatcgaa gttgtcgagt tttatgacga ccagagcggc tacgagcgcg
       61 ggctgacact gccgcttcgc catccttcag ggctgtttga tggtgaaacg gaagcggtat
      121 gggggctgaa taccgcgtac agcgtggtgg aaaaaagcgt caccactcgg gactacaact
      181 accgcaccgc cacagcagag atgatgacag agcagcatga cgccaccggg ggcgataaca
      241 ccacctacgg cgaagcctac cactatgccg acaacttcct gcaaaaaggc gataaagagg
      301 cagcggaaag cggcgcgttt tatgcccgta tccgccacga acgctacctg aatgaacagg
      361 ccattctcaa aggccagagc accagttcac tgctgatgcc ggggctggaa atcagggttc
      421 agggcgacga cgcgccagca gtgttccgca agggcgtact gattaccggc gtgacggcca
      481 gcgcggcgcg tgaccgcagc tatgagctga cgtttaccgc catcccgtac tcagagcgct
      541 acggctaccg cccggcgctt atcccccgcc cggtaatggc cggcacactc ccggcgcggg
      601 tcaccagcac ggtgaaaaac gatatctatg cccatatcga caaggacggg cgctaccggg
      661 tcaacctcga ctttgaccgc gatacgtgga aaccgggtta tgaaagcctg tgggtgcgcc
      721 agtcgcgccc gtatgcaggt gacacgtatg gcctg
//
LOCUS       DACADF010000385          753 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.402, whole genome shotgun sequence.
ACCESSION   DACADF010000385 DACADF010000000
VERSION     DACADF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.402"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            317..424
                     /locus_tag="ISB30_004992"
     CDS             317..424
                     /locus_tag="ISB30_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAP1992501.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVSWLNKRK"
BASE COUNT          201 a          169 c          228 g          155 t
ORIGIN      
        1 ggtgaaactc tgcggccttt ttcgtaagaa aaatgcaacg gttgcaagcg gataatattc
       61 tgcggaatgc gcagcacaga gtgaagaagt gatagattcc ggaacagaaa aacagtcgta
      121 gaatcagcgt caagccggaa gggttccggt gaggcgcaat gttgcggggg cttaatccct
      181 ggtggcattg gttgctggag agagaaaacc cccgcacgtt gcaggtatgc acctgacaac
      241 atcacggggg ctaatcttga acctgaacaa ctcaagaata gccgcgaaac gttgtcatta
      301 caacacaggc ggctatatga cgctcgcaga gctgggcatg gccttctggc atgatttagc
      361 ggctccggtc attgctggca ttcttgccag tatgatcgtg agctggctga acaagcggaa
      421 gtaacgtgtc atgcgggcgt caggctgccg taatggcaat ttgcgcccgg accaggccgc
      481 agaggtgaaa ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa gcggataata
      541 ttctgcggaa tgcgcagcac agagtgaaga agtgacggat tccggagcag aaaaacagtc
      601 gtagaatcag cggcaagccg gaaaggttcc ggtgaggcgc aatgttgcgg gggctttatc
      661 cctggtggca ttggttgctg gagagagaaa acccccgtac gttgcaggta tgcacctgac
      721 aacatcacgg gggctaatct tgaaccttaa caa
//
LOCUS       DACADF010000386          751 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.403, whole genome shotgun sequence.
ACCESSION   DACADF010000386 DACADF010000000
VERSION     DACADF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.403"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(421..>751)
                     /gene="intI1"
                     /locus_tag="ISB30_004993"
     CDS             complement(421..>751)
                     /gene="intI1"
                     /locus_tag="ISB30_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAP1992502.1"
                     /translation="WFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITK
                     PATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDAL
                     PPLTSER"
BASE COUNT          165 a          185 c          222 g          179 t
ORIGIN      
        1 tagatttacg tgcatagccg attttcattc ttttctcgct aattagttat ggggttattg
       61 ttatgttgat acagtaacga gttttgttac atgaggggag tcatttttcg ggagaagtca
      121 ggacttttca agactgtcac aaaaaccatc gtttttgata cattaattta accaataggt
      181 tgcagatcaa atcgcctgta acagcctttc tggctgtttg tcgttttcag aagacggctg
      241 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatcc atcaggcaac
      301 gacgggctgc tgccggccat cagcggacgc agggaggact ttccgcaacc ggccgttcga
      361 tgcggcaccg atggccttcg cgcaggggta gtgaatccgc caggattgac ttgcgctgcc
      421 ctacctctca ctagtgaggg gcggcagcgc atcaagcggt gagcgcactc cggcaccgcc
      481 aactttcagc acatgcgtgt aaatcatcgt cgtagagacg tcggaatggc cgagcagatc
      541 ctgcacggtt cgaatgtcgt aaccgctgcg gagcaaggcc gtcgcgaacg agtggcggag
      601 ggtgtgcggt gtggcgggct tcgtgatgcc tgcttgttct acggcacgtt tgaaggcgcg
      661 ctgaaaggtc tggtcataca tgtgatggcg acgcacgaca ccgctccgtg gatcggtcga
      721 atgcgtgtgc tgcgcaaaaa cccagaacca c
//
LOCUS       DACADF010000387          751 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.404, whole genome shotgun sequence.
ACCESSION   DACADF010000387 DACADF010000000
VERSION     DACADF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.404"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..673
                     /locus_tag="ISB30_004994"
     CDS             <1..673
                     /locus_tag="ISB30_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjhT family mutarotase"
                     /protein_id="HAP1992503.1"
                     /translation="VNENIFNGYFSDVELSKGNSALTEKVNRDYFSKPADDYFLNNHI
                     ISYDPSKNQWKNLGTTPFPGTAGSSVIFAEQQIYILGGERKPGLRSVRSWTGELSHDR
                     IKWSELPPVASPEGVSGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSHKGLDKAYS
                     KEIYQLIKNDWKKVGSLPEGLAYGVSLPWQGGMLILGGEKKDGKAVSDVIYLKKNDKQ
                     IKIVK"
BASE COUNT          233 a          114 c          189 g          215 t
ORIGIN      
        1 tgtaaatgag aatattttta atggatactt ttcagatgtc gagttatcaa aagggaattc
       61 cgcgctgaca gagaaagtca acagggatta ttttagtaaa cctgctgatg attatttttt
      121 aaataatcat ataatttctt atgacccgtc aaaaaaccag tggaagaacc tgggaacgac
      181 tccatttccg ggaactgctg gttcatcggt gatttttgca gaacagcaaa tttatatact
      241 gggaggggag agaaaaccgg ggcttcgttc tgttagatcc tggacggggg agctttcgca
      301 tgatcgaatt aaatggtctg aacttccacc agtagcttca cccgaggggg tgtcaggagc
      361 atatgcctct gttattgatg gaaatatctt tctggccggt ggtgcatatt tcccgggggc
      421 ggcagaaaag tatagcaatg gtgaatactg gtctcataaa ggactggata aagcctattc
      481 aaaagagata tatcagctaa taaaaaatga ctggaaaaaa gttggttctt tgccggaagg
      541 acttgcttat ggtgtaagcc tgccctggca gggggggatg ctcattctgg ggggggagaa
      601 aaaagatggt aaagctgttt cagatgttat ttatctgaaa aaaaatgaca aacaaataaa
      661 gattgttaaa taactgtaat gcaggtgact gtattagttg ttcagtcacc tgagttattt
      721 taatggggtt tataatgaat aaagatagat c
//
LOCUS       DACADF010000388          748 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.405, whole genome shotgun sequence.
ACCESSION   DACADF010000388 DACADF010000000
VERSION     DACADF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.405"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..>748)
                     /locus_tag="ISB30_004995"
     CDS             complement(<1..>748)
                     /locus_tag="ISB30_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1992504.1"
                     /translation="AFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWPT
                     GIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGFM
                     GDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKIIS
                     GYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVLI
                     GFDMPSRVQRWIHPEAALEYDTVDGKPFYGA"
BASE COUNT          276 a          189 c          132 g          151 t
ORIGIN      
        1 gctccataaa aaggttttcc atcgacagta tcatattcaa gcgcagcttc tggatggatc
       61 catctctgta cacgtgatgg catatcaaaa ccaataagga caagaacaac aaatgttaag
      121 ataaaagcaa gagaacacat tgctgtacca agacttgtat gagacgcaac aactgcacca
      181 agaataggcg cacatgctcc accaagtgaa cctacattat aaataaaccc cagtcctgcc
      241 gcacgctgct ccacattaaa ataaccactg attattttcg gcaccagagc accaatcccc
      301 tgaccaaaaa cctgctgagt aaagattaga aggccaagta accatacata gtcccggtcc
      361 acaagaaaaa cggggataat aacaagctga ccgataagaa gtgatataac atatgctttt
      421 cgggttccaa gccagtcccc cataaaacca cctatacaac ttccagctgc agagccaaaa
      481 cctgccagca taaatatgag agcaacagtt tctggtgaat actgcatatc aaccttaagc
      541 catgtaggaa gaaaagcctg cagtggccat ccgtagagga aacagaacat aaccacaagc
      601 atcaacataa tgcctgttgg ccatcttttc cccataccct gatatataaa actgataaaa
      661 acagccgcac agcacaatgc cataactgtg attaaagcag cgtttgcagt gacgcctgaa
      721 aaacaaacat atagacatgc aaatgcaa
//
LOCUS       DACADF010000389          741 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.406, whole genome shotgun sequence.
ACCESSION   DACADF010000389 DACADF010000000
VERSION     DACADF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.406"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(85..432)
                     /gene="tnpB"
                     /locus_tag="ISB30_004996"
     CDS             complement(85..432)
                     /gene="tnpB"
                     /locus_tag="ISB30_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP1992505.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(429..>741)
                     /locus_tag="ISB30_004997"
     CDS             complement(429..>741)
                     /locus_tag="ISB30_004997"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP1992506.1"
                     /translation="LVAASCEPGISISKLALENGINANLLFKWRQQWREGKLLLPSSE
                     SPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFRHGTLRFNGNVSEKLLTLLIQELK
                     R"
BASE COUNT          167 a          196 c          211 g          167 t
ORIGIN      
        1 agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt
       61 ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca gccgcttggg
      121 ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt
      181 gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca
      241 cagtccgtca ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat
      301 gaaaacatgg ccggacatgg gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc
      361 gttgaagcca tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg
      421 taacgggatc atcgcttcag ttcctgtatc agcagagtca ggagcttttc gctgacattg
      481 ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact gagggtttcc
      541 gggtcctctg cgagcgattc tggctgttcg gcagctgcat cgagagtcac aggaagtagc
      601 tgggggctct ctgaagaagg taatagcagc tttccctcgc gccattgttg tcgccatttg
      661 aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga tatcccgggt
      721 tcacaggagg cagcaacgag c
//
LOCUS       DACADF010000390          737 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.407, whole genome shotgun sequence.
ACCESSION   DACADF010000390 DACADF010000000
VERSION     DACADF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.407"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..340
                     /locus_tag="ISB30_004998"
     CDS             <1..340
                     /locus_tag="ISB30_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1X2 family transposase"
                     /protein_id="HAP1992507.1"
                     /translation="QRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWM
                     TDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
BASE COUNT          207 a          148 c          170 g          212 t
ORIGIN      
        1 tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg cgcacgtctt
       61 cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg cctttgaggt
      121 cgtggtatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca
      181 cgtaatcagc aagcgttaca ctcagcgcat tgagcgacat aacctgaatc tgagacaaca
      241 tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa
      301 agtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttacctgaac
      361 gttgtacact gtctcctaac tgagcgataa atttctgttc atcgtcaagt tgcatgtttt
      421 catttatgat taattcatac aaagcaagaa aaacagcttg atagtaacga ggacattctt
      481 gagatgaaga tgcattgccc agaatgtgtg atctaaagtt caaattattt gcatcaaata
      541 atttttgaat catctcataa acgaagaggt aattattgac aattttatct gcaccatttg
      601 tctgaatgta attctctaat tgagttcgtg tatggattgc atgactcgtc gaaccttcac
      661 cgtaaaatgt atcgagcgat gttgatcctg atgttggctt atcatctaaa aatatatagc
      721 ccaagatatt tgcaact
//
LOCUS       DACADF010000391          731 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.408, whole genome shotgun sequence.
ACCESSION   DACADF010000391 DACADF010000000
VERSION     DACADF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.408"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>731
                     /locus_tag="ISB30_004999"
     CDS             <1..>731
                     /locus_tag="ISB30_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAP1992508.1"
                     /translation="PPTPRHASPEQLEKTVRYLTQTVHPRSADNIDNLNRSAEYIKEV
                     FISSGARVTAQDIPITGGPYKNIVANYGPADGPLIIIGAHYDSVSSYENDQLTYTPGA
                     DDNASGVAGLLELARLLQQQAPKTGVQLVAYASEEPPFFRSDEMGSAVHAASLERPVK
                     LMIALEMIGYYDSTPGSQDYPSPAMSWLYPDRGDFIAVVGRMQDINAVRQVKAALLSS
                     RDLSVYSMNAPGFIPGIDFSDHLNY"
BASE COUNT          183 a          178 c          180 g          190 t
ORIGIN      
        1 taccaccaac gccccgacac gctagtcctg agcaattaga aaaaacagtt cgttatctta
       61 cacaaactgt gcatccacgt agcgccgaca atattgataa tctgaataga tccgcagagt
      121 atataaaaga ggtctttatc agtagcggtg ccagagttac cgcgcaggat atccccatta
      181 cgggtggccc ctacaaaaac attgttgcta attatggtcc tgccgatgga ccgctgatta
      241 ttattggtgc gcactatgac tctgtcagta gttacgaaaa cgatcaattg acctatacgc
      301 cgggcgcgga tgataacgcc agcggtgtgg caggattact cgaactggca cgtttgttac
      361 agcagcaagc accgaaaaca ggcgtgcagc tggtcgccta tgcgtcggaa gaaccgccct
      421 tctttcgtag cgatgaaatg gggagcgcgg tacatgcagc ttcgcttgag cgtccagtga
      481 aattaatgat agcgctggag atgattggtt attacgactc tacgcctggt agccaggatt
      541 acccatcccc ggccatgtcc tggctttatc ccgatcgggg agactttatt gccgtggtcg
      601 gcagaatgca ggatatcaac gccgttcgtc aggtaaaagc ggcattgttg tcatcccggg
      661 atttatctgt ttattctatg aatgccccag gttttattcc cggtattgat ttctctgatc
      721 accttaatta t
//
LOCUS       DACADF010000392          730 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.409, whole genome shotgun sequence.
ACCESSION   DACADF010000392 DACADF010000000
VERSION     DACADF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.409"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            74..>730
                     /gene="acrS"
                     /locus_tag="ISB30_005000"
     CDS             74..>730
                     /gene="acrS"
                     /locus_tag="ISB30_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HAP1992509.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     V"
BASE COUNT          219 a          146 c          166 g          199 t
ORIGIN      
        1 cattaggtga tagattaacc ttcgctattt tctcatcctg tgtcgaatat atttatttcc
       61 tgaataatta atcatggcaa aaagaaccaa agctgaagcc ctgaagaccc ggcaagaact
      121 gattgaaact gccatcgccc agtttgcgca gcatggcgta agcaagacga cgctcaacga
      181 cattgccgac gctgctaacg ttacgcgtgg cgctatctac tggcacttcg aaaacaagac
      241 tcaactgttt aatgagatgt ggttgcaaca gccttcattg cgggagttaa tccaggatca
      301 cttgaccgct gggttagagc atgacccgtt tcagcaattg cgtgaaaaat tgattgtcgg
      361 tttgcaatat attgccaaaa ttccccgcca gcaggcgttg ctgaaaatct tatatcacaa
      421 atgtgaattt aatgatgaga tgctggccga gggagtgata cgcgaaaaga tgggctttaa
      481 tccgcagact ctccgcgaag tattgcaggc gtgtcagcaa caaggttgtg tagcaaataa
      541 tctcgattta gatgttgtga tgattattat tgatggtgcc ttcagcggaa ttgttcaaaa
      601 ctggttaatg aatatggcgg gttatgatct ttataaacaa gcccccgctc tggtcgataa
      661 cgtattaaga atgttcatgc cagatgaaaa cataacgaaa ttaattcata aaacgaatga
      721 attaagtgtc
//
LOCUS       DACADF010000393          729 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.410, whole genome shotgun sequence.
ACCESSION   DACADF010000393 DACADF010000000
VERSION     DACADF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.410"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>729
                     /locus_tag="ISB30_005001"
     rRNA            <1..>729
                     /locus_tag="ISB30_005001"
                     /product="16S ribosomal RNA"
BASE COUNT          184 a          165 c          236 g          144 t
ORIGIN      
        1 ctagctggtc tgagaggatg accagccaca ctggaactga gacacggtcc agactcctac
       61 gggaggcagc agtggggaat attgcacaat gggcgcaagc ctgatgcagc catgccgcgt
      121 gtatgaagaa ggccttcggg ttgtaaagta ctttcagcgg ggaggaaggg agtaaagtta
      181 atacctttgc tcattgacgt tacccgcaga agaagcaccg gctaactccg tgccagcagc
      241 cgcggtaata cggagggtgc aagcgttaat cggaattact gggcgtaaag cgcacgcagg
      301 cggtttgtta agtcagatgt gaaatccccg ggctcaacct gggaactgca tctgatactg
      361 gcaagcttga gtctcgtaga ggggggtaga attccaggtg tagcggtgaa atgcgtagag
      421 atctggagga ataccggtgg cgaaggcggc cccctggacg aagactgacg ctcaggtgcg
      481 aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgtcga
      541 cttggaggtt gtgcccttga ggcgtggctt ccggagctaa cgcgttaagt cgaccgcctg
      601 gggagtacgg ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca caagcggtgg
      661 agcatgtggt ttaattcgat gcaacgcgaa gaaccttacc tggtcttgac atccacggaa
      721 gttttcaga
//
LOCUS       DACADF010000394          722 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.411, whole genome shotgun sequence.
ACCESSION   DACADF010000394 DACADF010000000
VERSION     DACADF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.411"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            92..688
                     /gene="fimE"
                     /locus_tag="ISB30_005002"
     CDS             92..688
                     /gene="fimE"
                     /locus_tag="ISB30_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAP1992510.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          195 a          137 c          176 g          214 t
ORIGIN      
        1 gttatctttt tatctattgg gctaattttg accgattgag gtttcctata ggtattcatt
       61 caaatatatc tcagttagga gtactactat tgtgagtaaa cgtcgttatc ttaccggtaa
      121 agaagttcag gccatgatgc aggcggtttg ttacggggca acgggagcca gagattattg
      181 tcttattctg ttggcatatc ggcatgggat gcgtattagt gaactgcttg atctgcatta
      241 tcaggacctt gaccttaatg aaggtagaat aaatattcgc cgactgaaga acggattttc
      301 taccgttcac ccgttacgtt ttgatgagcg tgaagccgtg gaacgctgga cccaggaacg
      361 tgctaactgg aaaggcgctg accggactga cgctatattt atttctcgcc gcgggagtcg
      421 gctttctcgc cagcaggcct atcgcattat tcgcgatgcc ggtattgaag ctggaaccgt
      481 aacgcagact catcctcata tgttaaggca tgcttgcggt tatgaactgg cggagcgtgg
      541 tgcagatact cgtttaattc aggattatct cgggcatcga aatattcgcc atactgtgcg
      601 ttataccgcc agtaatgctg ctcgttttgc cggattatgg gaaagaaata atctcataaa
      661 cgaaaaatta aaaagagaag aggtttgatt taacttattg ataataaagt taaaaaacaa
      721 at
//
LOCUS       DACADF010000395          716 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.412, whole genome shotgun sequence.
ACCESSION   DACADF010000395 DACADF010000000
VERSION     DACADF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.412"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..>716)
                     /locus_tag="ISB30_005003"
     CDS             complement(<1..>716)
                     /locus_tag="ISB30_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HAP1992511.1"
                     /translation="KSEPPKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIARE
                     IRDQGYRGGMTILRGFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFV
                     AVLGYSRMLYIEFTDNMRYDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTG
                     QHRFHPSLWQFGKEMGFSPRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGIT
                     VDVETANRHGLRWLHDVANQR"
BASE COUNT          171 a          188 c          197 g          160 t
ORIGIN      
        1 cgttggttag cgacatcgtg cagccagcgc agaccgtggc ggttggctgt ttcaacatcg
       61 acagtgatcc ccatcgggcg caggcgagtc attagtggga tgtaaaaact gttacgggtg
      121 tactgcacca tccgttccac cttaccttta gtctgtgccc tgaaggggcg acacagtcgg
      181 ggagagaagc ccatctcctt gccgaactgc cacagcgaag gatggaaccg gtgctgaccg
      241 gtctgatatg cgtcacgttg cagaaccaca gttttcatat tgtcatacaa cacttcgcgc
      301 ggcacaccac caaagaagcg gaacgcatta cgatggcagg tttccagcgt gtcataacgc
      361 atattgtcag tgaattcgat gtacaacatt cggctgtatc cgagaacagc aacgaacacg
      421 tgaagcggtg agcggccatt acgcatagtg ccccagtcaa cctgcatctg tcgtccgggt
      481 tcagtttcga accgaacggc aggctcctgc tcctgaggaa ccgagagaga acgaatgaat
      541 cccctgagaa tggtcattcc gccacgatat ccctggtctc tgatctcgcg agcgattacc
      601 gttgccggga ttttgtaagg atgagcatcg gcgatgcgtt gacgaatata atcccggtat
      661 tcatccagga gtgaagcaac agcaggtcgc ggcgtatatt ttggcggctc agattt
//
LOCUS       DACADF010000396          698 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.413, whole genome shotgun sequence.
ACCESSION   DACADF010000396 DACADF010000000
VERSION     DACADF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.413"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>698
                     /gene="tuf"
                     /locus_tag="ISB30_005004"
     CDS             <1..>698
                     /gene="tuf"
                     /locus_tag="ISB30_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP1992512.1"
                     /translation="ATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVE
                     MEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDK
                     PFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLD
                     EGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFK
                     GYRPQFYFRTTDVTG"
BASE COUNT          162 a          170 c          199 g          167 t
ORIGIN      
        1 gcgactgacg gcccgatgcc gcagactcgt gagcacatcc tgctgggtcg tcaggtaggc
       61 gttccgtaca tcatcgtgtt cctgaacaaa tgcgacatgg ttgatgacga agagctgctg
      121 gaactggttg aaatggaagt tcgtgaactt ctgtctcagt acgacttccc gggcgacgac
      181 actccgatcg ttcgtggttc tgctctgaaa gcgctggaag gcgacgcaga gtgggaagcg
      241 aaaatcctgg aactggctgg cttcctggat tcttacattc cggaaccaga gcgtgcgatt
      301 gacaagccgt tcctgctgcc gatcgaagac gtattctcca tctccggtcg tggtaccgtt
      361 gttaccggtc gtgtagaacg cggtatcatc aaagttggtg aagaagttga aatcgttggt
      421 atcaaagaga ctcagaagtc tacctgtact ggcgttgaaa tgttccgcaa actgctggac
      481 gaaggccgtg ctggtgagaa cgtaggtgtt ctgctgcgtg gtatcaaacg tgaagaaatc
      541 gaacgtggtc aggtactggc taagccgggc accatcaagc cgcacaccaa gttcgaatct
      601 gaagtgtaca ttctgtccaa agatgaaggc ggtcgtcata ctccgttctt caaaggctac
      661 cgtccgcagt tctacttccg tactactgac gtgactgg
//
LOCUS       DACADF010000397          697 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.414, whole genome shotgun sequence.
ACCESSION   DACADF010000397 DACADF010000000
VERSION     DACADF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.414"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..514
                     /gene="gadX"
                     /locus_tag="ISB30_005005"
     CDS             <1..514
                     /gene="gadX"
                     /locus_tag="ISB30_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAP1992513.1"
                     /translation="NLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVC
                     TVINNNIAHEWTLARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYG
                     VSIKRVAVSCGYHSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYG
                     TDRSAEGIRL"
BASE COUNT          202 a          129 c          162 g          204 t
ORIGIN      
        1 gaatcttcgc tatgcagaaa tgctacgtaa aagggcatta atctttgcgt tgttatctgt
       61 ttttcttgag gatacgcagt ttataccgtt acttctgaac gtgttgcaac cgaatatgcg
      121 aacacgagtt tgtacggtca tcaataataa tatcgcccat gagtggacat tagcccgaat
      181 tgctagcgaa ttgttgatga gtccaagcct gttaaagaaa aaattgcgcg aagaaggtac
      241 gtcatattca cagttgctta ctgagtgtag gatgcagcgt gctttacaac ttattgttat
      301 atatggggtt tcaattaagc gagttgcagt atcctgtgga tatcacagcg tgtcgtattt
      361 catttacgtt tttcgaaatt attatgggat gacgcccaca gagtatcagg agcgatcggc
      421 gcaggaatta ccgaactgtg gctcggcggc aagtatggct gcgcaaggga ttttttacgg
      481 cactgaccgt tctgcggaag gaataagatt atagagtttt actcagacat aaaaaaaacc
      541 cggcataggg gaccgggaag aggatagtct gccgtctcca gactaataaa ccgttataac
      601 actccctgtt ggcacgggaa actttgtgct ctcagtaagt taaatataac ttttactgga
      661 aataagatca gccatttttt tataaacata agctata
//
LOCUS       DACADF010000398          687 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.415, whole genome shotgun sequence.
ACCESSION   DACADF010000398 DACADF010000000
VERSION     DACADF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.415"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            20..>687
                     /locus_tag="ISB30_005006"
     CDS             20..>687
                     /locus_tag="ISB30_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992514.1"
                     /translation="MASIKNEHVSVLDSYKVRSFKETHSDTACIVRIIEIYSLNKLRA
                     KGEKLYSLTGLTVPDTETVANEINLLLSRYAQLCRQEEEELSFRQREVTNAEVAWKST
                     FSKNGVSSIAEAKTNKMGHAERADAERYYHLAVSRLNEQHSRLSTIKLLPGVLADEGN
                     YIGKGIDKRLLNIFPQSGQIPADFISVFNDSDVVRDIKFITDALKSLSDSVSEIISRC
                     SVPT"
BASE COUNT          207 a          123 c          166 g          191 t
ORIGIN      
        1 caatgttttt aagaagaaat tggcatcaat taagaacgaa catgtgtcgg tgttagatag
       61 ttacaaagta agatcattta aagagactca tagcgacacg gcctgtattg tgcgtattat
      121 tgaaatatat tcactgaata agctgcgagc taaaggtgaa aagctgtatt cacttacggg
      181 gttaacagta cctgatacgg aaactgtggc gaatgagatc aacctgctcc tgagccgtta
      241 tgcccagttg tgtcgtcagg aagaggaaga gttatctttc cggcagcgtg aagtgacaaa
      301 tgcggaagtt gcatggaaaa gcacattctc aaaaaacggc gtcagtagca ttgctgaagc
      361 caaaacgaat aaaatggggc atgctgaacg agcggatgct gaacggtatt accatctggc
      421 tgtttcccgg ttgaatgaac aacatagtcg attgagcacg atcaagcttt tgccgggagt
      481 actggccgat gaaggaaact acattgggaa gggaatcgat aagcgtttac tgaatatatt
      541 tcctcaatcc ggccagatcc ctgctgattt tatttctgta tttaatgaca gtgacgttgt
      601 ccgtgatatt aaatttatta ctgatgcact caagtcttta tctgattccg tgagtgaaat
      661 tattagtcgt tgtagcgttc caactga
//
LOCUS       DACADF010000399          677 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.416, whole genome shotgun sequence.
ACCESSION   DACADF010000399 DACADF010000000
VERSION     DACADF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.416"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>677
                     /locus_tag="ISB30_005007"
     CDS             <1..>677
                     /locus_tag="ISB30_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP1992515.1"
                     /translation="NIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELNTSLKFV
                     IERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSNVNRTIA
                     NVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRDVTEEEG
                     NIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGLVDALED
                     DDDVQKV"
BASE COUNT          227 a          118 c          147 g          185 t
ORIGIN      
        1 ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt tatgcaaaat
       61 tcggtgtaga aatctatgct gctgccaaac aaggtgaacc cgatccagaa ttaaacacat
      121 ctttaaaatt cgttattgaa cgtgcaaaac aagcacaagt tccaaagcac gttattgata
      181 aagcaattga taaagccaaa ggcggcggag atgaaacgtt cgtgcaggga cgttatgaag
      241 gctttggtcc taatggctca atgattatcg ctgagacatt aacttcaaat gttaaccgta
      301 cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga gcggcaggtt
      361 ctgtcagcta tatgtttgac aatacgggtg tgattgtatt taaaggaaca gaccctgacc
      421 atatttttga aattttgctt gaagctgaag ttgatgttcg tgatgtaact gaagaagaag
      481 gtaatattgt tatttatact gaacctactg accttcataa aggaatcgcg gcgctaaaag
      541 cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa tctgaagttg
      601 agctttcccc agaagattta gaaatctttg aagggcttgt tgatgccctt gaagatgacg
      661 acgatgtaca aaaagtt
//
LOCUS       DACADF010000400          668 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.417, whole genome shotgun sequence.
ACCESSION   DACADF010000400 DACADF010000000
VERSION     DACADF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.417"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            70..>668
                     /locus_tag="ISB30_005008"
     CDS             70..>668
                     /locus_tag="ISB30_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAP1992516.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDADNRVQFVNQTALKTLGVVQNN"
BASE COUNT          185 a          134 c          160 g          189 t
ORIGIN      
        1 atcatcatga tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa
       61 ggtaaagcta tggagcttgc gactacgcag tcagtattga tgcaaattca accaacaatt
      121 cagcgttttg ccagaatgct tgccagcgtt ttgcagcttg aggttgagat cgttgatgaa
      181 aacttgtgtc gcgtcgccgg aacgggcgcg tatgggaagt ttcttggccg ccagttgagc
      241 ggcaactcac gtctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag
      301 tctcgcttcg atcccctttg tgaaggttgc gatagtaaag aaaattgccg cgaaaaagca
      361 tttctgggta cgccagtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc
      421 gttacccacg agcaacaaga acatatcagt gataatttac gcgaattttc cgattacgtt
      481 cgccatatat ccaccatttt tgtttctaaa cttctggagg atcagggacc aggagataac
      541 atcagtaaaa tattcgcgac catgatcgat aatatggatc agggcgtgtt agtggttgat
      601 gctgataatc gggttcagtt tgttaatcag acagctttaa aaacactcgg tgttgtacaa
      661 aataatat
//
LOCUS       DACADF010000401          666 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.418, whole genome shotgun sequence.
ACCESSION   DACADF010000401 DACADF010000000
VERSION     DACADF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.418"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>666
                     /locus_tag="ISB30_005009"
     CDS             <1..>666
                     /locus_tag="ISB30_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HAP1992517.1"
                     /translation="QGAVESSRLRSMQEKSQYQTVDENGRPVGGGIVPDSLRDENSDL
                     GHWYGAIETEVDGYFSGVKYNSGGGRIAGSYDWILGNCDTEDAQKFRGRGFKQLTGRS
                     NYAEYWVYRAWIDTNSFTAKWWEDPLWRLHDRRRLTRIPANIEEPHRVTRSEYNCIDT
                     GGFFIVKTVDRRGTRSSITRAMDQDSEVIHLNNTTIDTTDEQTIRSVSRAINGGENGL
                     PDR"
BASE COUNT          189 a          116 c          184 g          177 t
ORIGIN      
        1 ggcaaggggc agtggaatct tcccgtttaa gaagtatgca ggaaaaaagc cagtatcaaa
       61 ctgttgatga aaatggtcgg ccagtcggcg gaggtattgt tccggactca ttacgtgatg
      121 agaattcgga cttgggtcat tggtatggtg caattgagac tgaggtcgat ggatacttct
      181 ctggtgttaa atataattct ggtggaggac gaattgcagg ctcatatgac tggatactag
      241 gaaactgcga cactgaggat gcccagaagt ttcgtgggcg aggttttaag cagttaactg
      301 gtcgatcaaa ttatgccgaa tattgggtct atcgtgcctg gatagatacg aatagtttta
      361 ccgctaagtg gtgggaagac cctctgtggc gcttacatga tcgtcgtcgt ttaactcgca
      421 taccggctaa tatcgaagag cctcacaggg ttaccagaag cgaatataac tgtatcgata
      481 ctggtggatt ctttattgtt aagaccgttg atcgtcgggg tactcggtca agtattacca
      541 gagcgatgga tcaagacagt gaagttattc atttgaacaa taccacgata gatacaacgg
      601 atgaacaaac aataagaagt gtgtcccgtg caattaatgg tggtgaaaat ggtttgccag
      661 acagga
//
LOCUS       DACADF010000402          664 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.419, whole genome shotgun sequence.
ACCESSION   DACADF010000402 DACADF010000000
VERSION     DACADF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.419"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>664
                     /locus_tag="ISB30_005010"
     CDS             <1..>664
                     /locus_tag="ISB30_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992518.1"
                     /translation="GADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDY
                     QATEIAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIA
                     DQMMSNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDL
                     CLDPRRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQ
                     VR"
BASE COUNT          135 a          181 c          204 g          144 t
ORIGIN      
        1 gcggggccga ctatctggta tggcgcaatg ggaggggagc agtccggttg ctcggtcgtg
       61 agaacaatct gatgttgctc gaatatgccg gggagcgaat gctctctcac atcgttgccg
      121 agcacggcga ctaccaggcg accgaaattg cagcggaact aatggcgaag ctgtatgccg
      181 catctgagga acccctgcct tctgcccttc tcccgatccg ggatcgcttt gcagctttgt
      241 ttcagcgggc gcgcgatgat caaaacgcag gttgtcaaac tgactacgtc cacgcggcga
      301 ttatagccga tcaaatgatg agcaatgcct cggaactgcg tgggctacat ggcgatctgc
      361 atcatgaaaa catcatgttc tccagtcgcg gctggctggt gatagatccc gtcggtctgg
      421 tcggtgaagt gggctttggc gccgccaata tgttctacga tccggctgac agagacgacc
      481 tttgtctcga tcctagacgc attgcacaga tggcggacgc attctctcgt gcgctggacg
      541 tcgatccgcg tcgcctgctc gaccaggcgt acgcttatgg gtgcctttcc gcagcttgga
      601 acgcggatgg agaagaggag caacgcgatc tagctatcgc ggccgcgatc aagcaggtgc
      661 gaca
//
LOCUS       DACADF010000403          660 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.420, whole genome shotgun sequence.
ACCESSION   DACADF010000403 DACADF010000000
VERSION     DACADF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.420"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..402
                     /locus_tag="ISB30_005011"
     CDS             <1..402
                     /locus_tag="ISB30_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP1992519.1"
                     /translation="VRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLII
                     DEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRI
                     LHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            470..660
                     /locus_tag="ISB30_005012"
                     /pseudo
     CDS             470..660
                     /locus_tag="ISB30_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          176 a          168 c          181 g          135 t
ORIGIN      
        1 gtccgtgcag gtatcaaagt tcgcttcaca acagcagcag atctgttact tcagttatct
       61 acggcacaac gtcagggccg ttataaaacg acgcttcagc gtggagtaat ggccccccgc
      121 ctgctcatca ttgatgaaat aggctatctg ccgttcagtc aggaagaagc aaagctgttc
      181 ttccaggtca tcgctaaacg ttacgaaaag agcgcaatga tcctgacatc caatctgccg
      241 ttcgggcagt gggatcaaac gttcgccggt gatgcagcac tgacctcagc gatgctggac
      301 cgtatcttac accactcaca tgtcgttcaa atcaaaggag aaagctatcg actcagacag
      361 aaacgaaagg ccggggttat agcagaagct aatcctgagt aaaacggtgg atcaatattg
      421 ggccgttggt ggagatataa gtggatcact tttcatccgt cgttgacagg atgcatacgc
      481 cgggttcaac gagctgtacc gggatggccg gataacggaa gccgcctgtt gggctcacgc
      541 ccgccgtaaa atccacgatg tgcacgttcg caccccgtca gccctgacgg aggaagcgct
      601 gaaacggatc ggcgaactgt acgccatcga ggcagagata aggggaatga cggcggagca
//
LOCUS       DACADF010000404          659 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.421, whole genome shotgun sequence.
ACCESSION   DACADF010000404 DACADF010000000
VERSION     DACADF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.421"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..452)
                     /locus_tag="ISB30_005013"
     CDS             complement(<1..452)
                     /locus_tag="ISB30_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HAP1992520.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFE"
BASE COUNT          199 a          120 c          135 g          205 t
ORIGIN      
        1 cattcgaata agtaacgaac gcctttcttg cctgatttat aggtgagtat tttgtatcca
       61 tcgtaatcat atttacagga tgttgtttca ttatttctat ggaattcgac aatgccgtcc
      121 tcaatgccaa tcatttcgat atctgtcgca taacctttgc gataataatc tttaatttca
      181 gcaaatgttt tggttttatc tgcatccgct ttcttctgaa agacggggtc aagtttgcca
      241 ctttgcagta aagggtaaac ggattgccag actccatccc agtcactgag cgttcggttt
      301 tgtacattgg tatcatcaaa aacaccatta gcagcttttt gttcgacctc tgttaagggt
      361 ttgccgtgtg agtgatgacc atgcgaaaag gcaggagcgc taacaataaa gacacctaaa
      421 gcaacagcca gtttatgaag acgaatcgcc aaaacaattt cctccagagt taatgtacat
      481 gatgtgtaat gttatattgt aacatatgaa aaaatgcaac aactcattag gaaatgagag
      541 catggtattc tcaggtgtat tgaactatct tattgaaata aaataaatta ttctggtgat
      601 gaaaggttga tgacaaacga aacataactt gatgcgatct gtgtgggccg gatagggcg
//
LOCUS       DACADF010000405          659 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.422, whole genome shotgun sequence.
ACCESSION   DACADF010000405 DACADF010000000
VERSION     DACADF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.422"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            255..>659
                     /locus_tag="ISB30_005014"
     CDS             255..>659
                     /locus_tag="ISB30_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992521.1"
                     /translation="MKDNIHTAVIMAGEFLSANEWIRRKNQLKTSVHPASVASFAPYG
                     FNINQCDDDGNLWTNFPRPHIKLKIAPGKTACFLCRREELLFGYDEAHNHYQPNLNLV
                     LFVKNHGLHKDVYLKSHDDKSEYDSRILCNEKF"
BASE COUNT          197 a          139 c          127 g          196 t
ORIGIN      
        1 ttttagaatt ttttgtgcga gaagttgcct ttaagggtaa gggaattgtt cgttctacat
       61 taccagcaga tgcccatttt tataccggtg ggtcaaaagc cctgcgttat cacctacaac
      121 caggaaacca atggccaaac tatatatggc atgatgatat aggtgatatt gtaccgtttc
      181 ctctaccctc acttccgctt acatttaccg ctaaccgacc cacatttaat tccagagaat
      241 tagcggataa atggatgaag gataatattc ataccgctgt catcatggcg ggggaatttc
      301 tgtctgctaa tgaatggatt cgccgcaaaa accagcttaa aactagtgta caccctgcgt
      361 cagttgcttc tttcgcaccc tatggcttta acattaacca gtgtgatgac gatggcaatc
      421 tttggactaa tttcccacgt ccccacatca aattaaaaat cgcaccagga aaaacagctt
      481 gttttctctg ccgccgggaa gagcttttat ttggttatga tgaagcacat aatcactacc
      541 agccgaatct taatctggtt ttatttgtga aaaatcatgg gttgcataag gatgtttacc
      601 taaaatctca tgacgataaa agtgaatacg atagcagaat attatgtaac gaaaaattt
//
LOCUS       DACADF010000406          653 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.423, whole genome shotgun sequence.
ACCESSION   DACADF010000406 DACADF010000000
VERSION     DACADF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.423"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(44..607)
                     /locus_tag="ISB30_005015"
     CDS             complement(44..607)
                     /locus_tag="ISB30_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP1992522.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          202 a          139 c          134 g          178 t
ORIGIN      
        1 ggaaaacgta ggaatctgac attttgaatc agaaggtact gttttaacga ggcttttttt
       61 tccacacccc tttaaaacga gcagcattac tggctgtgta tctgacagta tgctgaatat
      121 ttcgatgacc cagataatcc tgaagtagtc gggtatcgac accattgtca gccagagcaa
      181 aaccacaggc atgacgtaac atatgaggat gtgcttttac agctaatcct gcacgttttc
      241 cagcctcacg aatgatagaa aaaaaatgtt gtctgctgat agggcggcgt ttccgggaga
      301 gaaaaagcca gtctccttca actccgtttt caattaactt cctctgctta agccacaact
      361 tgatcaaatt atattcatca ggaagaagag gatgtgttgt cgagaaacca tttttaatac
      421 gccgaatatt aagttgtttt ccagatgcat caatatcgga taaacgtaaa tcaagaagtt
      481 cactggccct aaaaccgtga atgaaagcca tcataattag acagcgattt ctttcaggaa
      541 aggacatgct ctgggctgca tccatcagcc tgtagacttc atcctgtgtg agatattttc
      601 tcgtcatacg ccaccgtgta cagttgtaaa aaaaagagag gcacacccag ata
//
LOCUS       DACADF010000407          649 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.424, whole genome shotgun sequence.
ACCESSION   DACADF010000407 DACADF010000000
VERSION     DACADF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.424"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..200
                     /locus_tag="ISB30_005016"
     CDS             <1..200
                     /locus_tag="ISB30_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992523.1"
                     /translation="RTDSREDRRAKKISLTSEGMFFIQRLTLAQSMYLKEIWDYLTHD
                     EQELFEVINKKLLAHFSDASS"
BASE COUNT          216 a          112 c          145 g          176 t
ORIGIN      
        1 aaaggacgga tagcagggag gatcgccgtg ctaaaaaaat cagtctgaca tctgaaggta
       61 tgttttttat tcagaggctc actcttgcac aaagcatgta tctgaaagaa atctgggatt
      121 atctgaccca tgatgaacag gaactgtttg aagtcattaa taaaaagtta ctggcacatt
      181 tttctgatgc cagctcataa agtgcgaaat atctgaggat gctggatagc ttcaggcaag
      241 agaataatga ttcttgcaga tgtgtttttc cggatacaaa aacaaaagta aaaaattgca
      301 gcgccaggcc acctttcaaa gcagggaggc ctgtaccgcg tcgaaaattt cagccagtta
      361 atgtaattgt ctgaaccagg cacttcgccc gggcaggaga aggagcgtgg ttgcggtcat
      421 cctgtatact gaagtcgtta tgaaggaata atacaaataa ttgcccgttt tctctgtacg
      481 atagaggcag gtgtgtatga ttgcattact atgtttttgt gatataaaaa actaaatggt
      541 gagatatgca ttttaaaata taacaaaaat caccatgaaa taagaataat tatccacgga
      601 gaacataaac tgcagactgt tacaatgcat gctgttcatc ttgacaatg
//
LOCUS       DACADF010000408          644 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.425, whole genome shotgun sequence.
ACCESSION   DACADF010000408 DACADF010000000
VERSION     DACADF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.425"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..461
                     /locus_tag="ISB30_005017"
     CDS             <1..461
                     /locus_tag="ISB30_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992524.1"
                     /translation="QMVVSAIASTPQTEFSWFRYGQYNETGIYISIEKTIIITITVSG
                     GCFKKEYSIVSHMLVTDTITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMT
                     LRLRLQDQQEETSVTLFQGKISTDGNNYSIIKGKVSSVLLLLKMLQINHA"
BASE COUNT          190 a          162 c          123 g          169 t
ORIGIN      
        1 aacaaatggt tgtgtccgct attgccagta cacctcaaac tgagttctca tggttccgat
       61 acggacagta taacgaaacg ggtatttaca tctcgatcga gaagaccatt atcattacaa
      121 tcaccgtttc tggtgggtgc tttaaaaaag aatacagcat tgtctcccac atgctggtca
      181 ctgacaccat aacagaggcc accctgtacg aaaacgggct gtatacgcgc cacatcagac
      241 tcagtcgccc agtatctgac agcaaatacc ccttgccacc cggatctcag ctcattaaaa
      301 atatgacctt acgactgcgc ctgcaggatc agcaggaaga aacatccgtg acattatttc
      361 agggaaaaat aagtaccgac ggaaataact attccatcat caaaggtaaa gtaagctcag
      421 tacttctgtt attaaaaatg ctgcagatta atcatgcctg atctctgtcc cggtatttta
      481 gcccgaggta tattctgaaa tatcattcat catgaccggc gtttatacaa acgccagtca
      541 ttcgctaaca gcttcaccgt tccttccccc atccaggttc cgcatgagcg tgttgtaatg
      601 ttcataagcg tattatcagg attgtctgga aaattcccga tact
//
LOCUS       DACADF010000409          643 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.426, whole genome shotgun sequence.
ACCESSION   DACADF010000409 DACADF010000000
VERSION     DACADF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.426"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..322
                     /locus_tag="ISB30_005018"
     CDS             <1..322
                     /locus_tag="ISB30_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP1992525.1"
                     /translation="ASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHAGR
                     GVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAERL
                     ELEI"
BASE COUNT          112 a          207 c          197 g          127 t
ORIGIN      
        1 cgcgagttgc agcgacggca tcgtcggctg ttgcaccttg tcggccgagg atcccgagtt
       61 ctggcccgac gccctcaagg gggaggccgc atatctgcac aagctcgcgg tgcgacggac
      121 acatgcgggc cggggtgtca gctccgcgct gatcgaggct tgccgccatg ccgcgcgaac
      181 gcaggggtgc gccaagctgc ggctcgactg ccacccgaac ctgcgtggcc tatacgagcg
      241 gctcggattc acccacgtcg acactttcaa tcccggctgg gatccaacct tcatcgcaga
      301 acgcctagaa ctcgaaatct aacgtccgtt cgggcatcga ggtccatgtc ggggtgggac
      361 gggcccgtgg cttcaagatc acttgcagtc cgaccgcgat gtcttggttg cgcgagaggt
      421 tgtcgatatc ctccacttcc atcatcaacc ctggataatg ccgccgccgt catcgccgcc
      481 gacgcccgtg ccgggctttt cgggcctgtc aggcttgctc ggccttcagc ctgcctgggc
      541 gagatctccg gcggacggat taacggcgga gcttcgccgc ctttcgtgcg tgtgaaggcc
      601 gaagatagtt ctctcaaaaa catccgttta tgagagatac caa
//
LOCUS       DACADF010000410          637 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.428, whole genome shotgun sequence.
ACCESSION   DACADF010000410 DACADF010000000
VERSION     DACADF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.428"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>637
                     /locus_tag="ISB30_005019"
     CDS             <1..>637
                     /locus_tag="ISB30_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAP1992526.1"
                     /translation="IAVSELAKRVSGKTNRKLVATMLSLAVAGTVNAANIDISNVWAR
                     DYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGAAASGAATAIGGSYSVT
                     VAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVVETVGATPAETNPTTYS
                     DALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAYSHDLLSASLF"
BASE COUNT          203 a          125 c          129 g          180 t
ORIGIN      
        1 cattgctgtt tctgaattag cgaaaagagt ttctggtaaa acaaaccgaa aacttgtagc
       61 aacaatgttg tctctggctg ttgccggtac agtaaatgca gcaaatattg atatatcaaa
      121 tgtatgggcg agagactatc ttgatcttgc acaaaataaa ggtattttcc agcccggagc
      181 aacagacgta acaatcactt taaaaaacgg agataaattc tctttccata atctctcaat
      241 tccggatttt tctggtgcag cagcgagtgg cgcagctacc gcaataggag gttcttatag
      301 tgttactgtt gcacataaca aaaagaaccc tcaggccgca gaaactcagg tttacgctca
      361 gtcttcttac aaggttgttg acagaagaaa ttccaatgat tttgagattc agaggttaaa
      421 taaatttgtt gtggaaacag taggtgccac cccggcagag accaacccta caacatattc
      481 tgatgcatta gaacgctacg gtatagtcac ttctgacggt tcaaaaaaaa tcataggttt
      541 tcgtgctggc tctggaggaa catcatttat taatggtgaa tccaaaatct caacaaattc
      601 agcatatagc catgatctgt taagtgctag tctattt
//
LOCUS       DACADF010000411          635 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.429, whole genome shotgun sequence.
ACCESSION   DACADF010000411 DACADF010000000
VERSION     DACADF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.429"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            82..>635
                     /locus_tag="ISB30_005020"
     CDS             82..>635
                     /locus_tag="ISB30_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAP1992527.1"
                     /translation="MSKKLGFALSGLMLAMVAGSASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEISEGVLDTTVGAKAKPFSITVDCSKANPAPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINTPSDGVNIALHNIEGSTIKQVKINNPGDIYSKALDATSKS
                     ATYDFKASYVRADASLAATAGYVKT"
BASE COUNT          179 a          128 c          142 g          186 t
ORIGIN      
        1 atctcttttc ctctatcggg aatccctgcc tgaaagggcc caattgtagt taaactcatg
       61 gaatatgatt caaggatttt tatgtctaaa aaattaggtt ttgcgctctc tggtcttatg
      121 ctggcaatgg ttgcaggtag tgcttctgca gatatggacg gtggtcagtt aaatatcagt
      181 ggtctggttg ttgataatac ctgtgaaacc cgcgtagacg gcggtaataa agatggcctg
      241 atcctgctgc aaactgcaac cgttggagaa atctctgagg gtgtgctgga cactaccgtt
      301 ggtgctaagg ctaaaccatt cagcattact gttgactgta gtaaagctaa tccggcacca
      361 ggcagcacag caaaaatgac ttttggttca gtcttctttg gtaacagtaa aggaaccctg
      421 aataatgaca tgtcaattaa tactccatct gacggtgtta atattgctct gcacaatatt
      481 gaaggttcta ctattaagca ggttaaaatc aacaatcctg gcgatatcta ttccaaagca
      541 ctggatgcaa cttccaaatc cgcaacttat gattttaaag cgtcttatgt tcgtgcagat
      601 gcaagtctgg cagccacagc aggttatgta aaaac
//
LOCUS       DACADF010000412          631 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.430, whole genome shotgun sequence.
ACCESSION   DACADF010000412 DACADF010000000
VERSION     DACADF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.430"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            121..366
                     /locus_tag="ISB30_005021"
                     /pseudo
     CDS             121..366
                     /locus_tag="ISB30_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT          206 a          127 c          127 g          171 t
ORIGIN      
        1 agccaaataa ccatcaatga tctagcgaat gtagaaatat ctgctccttc gctaaatgtt
       61 caagatttaa tagtcaagag tctcaaagct tttgatgaca aaatcacaac actttcatcc
      121 atgaaccaaa ccttggaaca aatgtcccaa accctgttca aatcctggtt tgtggatttt
      181 gatccagtga ttgataacgc tctggatgca ggaaatccaa tcccggaagc cctgcaaact
      241 cgcgccaaat tgcgtcaaaa agtacgtaat agtgcagatt ttaaaccgct tccggcggaa
      301 attcgctcgc ttttcccaag tgaatttgaa gaaacggagt tgggttgggt gccaaagggg
      361 tggaaagaag ggacgcttcc agagatagct tttataaact ccacatcgtg gactaataaa
      421 aatcaaccag attatatcaa ttatgtagat ctctctaatg cgaaagatgg cagaatatac
      481 tcaattgaaa aattatcatt taatgatgct ccaagtcgag ctcgaagaat attaaaaaaa
      541 gatgatctga tttttggcct tgtcagacct gctaacagat catttgcata tgtacatgtt
      601 gatggtctaa cgggtagcac aggctttgcc g
//
LOCUS       DACADF010000413          615 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.431, whole genome shotgun sequence.
ACCESSION   DACADF010000413 DACADF010000000
VERSION     DACADF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.431"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..610)
                     /locus_tag="ISB30_005022"
     CDS             complement(<1..610)
                     /locus_tag="ISB30_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1992528.1"
                     /translation="MQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVLTEVQ
                     HEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLACGLA
                     PGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGLAAQV
                     YSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQE"
BASE COUNT          209 a          160 c          110 g          136 t
ORIGIN      
        1 tctcttgttg ccttttttgc cagtcatcag actccggaag attcttgcga agataaaaag
       61 caaataatat tggaagcatg ccgacaaaga aaagagaacg ccagccccat agtggtacta
      121 caatgctata aacctgtgca gctaaacctc ccccaataga aaatccagaa attaaaaaac
      181 cacttgcctt gtttctgaga tgaacaggcc agctttcgat aacatatgtt acacttgagc
      241 catattcacc tgccatacca agcccaataa caatgcgagc aataaacatc actgcatatc
      301 caggagccag accacaggca agggttccga gagagaatag cactatactt aaaaccattg
      361 ccatcctgcg ccccatcttg tccgataaag cgccaatagc aagcccccca aaccatcgag
      421 aaataaaagc agcggaaata aggcttgccg cttctattgt cgtcaagccg aattcatgct
      481 gaacctctgt aagaaccaga gatatcagaa cgaaatcaaa accatcaagt aaataacctg
      541 tccatgccgc accgaatatc ttccactgtc tgggtgaaag atgcttatac caaacctgct
      601 ttttctgaat acagg
//
LOCUS       DACADF010000414          613 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.432, whole genome shotgun sequence.
ACCESSION   DACADF010000414 DACADF010000000
VERSION     DACADF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.432"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..>613)
                     /locus_tag="ISB30_005023"
     CDS             complement(<1..>613)
                     /locus_tag="ISB30_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP1992529.1"
                     /translation="SSLFENTGDGIIYTGIVPLITVVEPVVSNQHSVLIIPGGGYRRI
                     VYEREGLDVGRWIAGQGYTAFILSYRMPSVNGYYSFADAKNALNFISYWAGMSERTFK
                     SVGCMGFSAGGHLAGYITHLHYEEETSVRPDWMALIYPVISLDRSIAHQGCRKRLLDA
                     GESEYSASLENSVTEYMPPVFIVHATDDKDVPVIHSLRYFNSIR"
BASE COUNT          201 a          154 c          101 g          157 t
ORIGIN      
        1 cccgaataga gttaaaatat ctcagagaat gaataactgg aacatcctta tcatctgttg
       61 catgcacaat aaagactggt ggcatatact ctgtaacact attttcaaga ctggctgaat
      121 attcactttc acctgcatcc agaagacgtt tccggcatcc ctgatgagca atactcctgt
      181 caagagatat aactggatat atcagagcca tccagtcagg acgcacagaa gtctcttcct
      241 cataatggag atgagtaata tatccagcaa gatgaccacc tgccgaaaac cccatacatc
      301 caacactctt aaatgttctc tccgacattc cggcccagta agaaataaag ttcagggcat
      361 tcttcgcatc tgcaaaggaa taataaccat taacggaggg cattctgtaa gaaagaataa
      421 atgccgtata tccctgacct gctatccatc tgccaacatc aagtccctct ctttcataaa
      481 caatcctcct gtatccacct ccaggtataa tcagcacact atgttgatta gatacaacag
      541 gctcaacaac tgtaataagg ggcacaatac ctgtatatat tatgccatcg ccagtattct
      601 caaatagcga act
//
LOCUS       DACADF010000415          609 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.433, whole genome shotgun sequence.
ACCESSION   DACADF010000415 DACADF010000000
VERSION     DACADF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.433"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..347)
                     /locus_tag="ISB30_005024"
     CDS             complement(<1..347)
                     /locus_tag="ISB30_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP1992530.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVA"
BASE COUNT          163 a          155 c          130 g          161 t
ORIGIN      
        1 tgtgccacag ccgacaggat ggtgaccacc atttgcccca tatcaccgtc ggtactgatc
       61 ccgtcatcaa tgaaccggac tgccacgccc tgagcgtcaa attcctttat cagttggatc
      121 atatcggcag tgtcgcggcc aagacggtcg agcttcttaa ccagaatgac atcaccttcc
      181 tccaccttca tcctcagcaa atccagccct tcccgatctg ttgaactgcc ggatgcctta
      241 tcggtaaata tacggtttgc tttcacacct gcgtctttga gtgctctgac ctgaagatca
      301 agagactgct gactggttga gacccgagcg taaccaaaaa gtcgcataaa aatgtacctt
      361 aaatcgaata tcggacaact catgtctatt attacaaatt tacgatttaa tagacatatt
      421 aatgtaacag ttttacgatg tccgataatt tataacattt cgtacggttg gaaaaatgtt
      481 actaaatgcc cgtcaggcag ggaggccgat atgcccgttg actttctgac cactgagcag
      541 actgaaagct atggcagatt caccggtgaa ccggatgagc ttcagctggc acgatatttg
      601 gcactgttg
//
LOCUS       DACADF010000416          607 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.434, whole genome shotgun sequence.
ACCESSION   DACADF010000416 DACADF010000000
VERSION     DACADF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.434"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..411
                     /locus_tag="ISB30_005025"
     CDS             <1..411
                     /locus_tag="ISB30_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAP1992531.1"
                     /translation="SLFLANIKSVSAMPEELKDRNTLQFAMKARLKLFWRPASLDNSD
                     ALTAWEKLKFHKEAGKLIVKNPTPFYISFSDLTVSGKNIVPTESKSEPGALLMMVGPF
                     SEQRFSLPVGAGSIVEWSVITDYGSSSGKRQQKL"
BASE COUNT          185 a          111 c          132 g          179 t
ORIGIN      
        1 agtttgtttc tggcaaacat aaaatctgtt tctgcgatgc cagaagaact aaaagataga
       61 aataccttgc aatttgcaat gaaagcccgg cttaaattat tttggcggcc agcttctttg
      121 gataatagtg atgctctcac tgcatgggaa aaacttaagt tccataaaga agcggggaag
      181 ctcatagtaa aaaatccaac gcctttttat atttcattca gtgatctgac agtatcggga
      241 aaaaatattg ttcccaccga gagcaaatct gaacctggcg cattattgat gatggtagga
      301 ccattttctg agcagcggtt tagtcttcct gtcggagcgg gaagtattgt tgaatggtcg
      361 gtgataactg actacggttc aagttctggg aaacgacaac aaaagttata aatcaccgaa
      421 gtgtattact ataaataacc gatctgtatt tataatatta cgggcgcaag ggaatttatg
      481 ctgctaccat tgcatcgcac atttgtctta actgggataa catttgcatt atcggcagtg
      541 tattcattga gctatgcacg agatgaattt aaccttcgta tcctggagct ggatagccca
      601 cttgaaa
//
LOCUS       DACADF010000417          603 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.435, whole genome shotgun sequence.
ACCESSION   DACADF010000417 DACADF010000000
VERSION     DACADF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.435"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>603
                     /locus_tag="ISB30_005026"
     CDS             <1..>603
                     /locus_tag="ISB30_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HAP1992532.1"
                     /translation="NWTHAIYFRFVIADYFISKVAKVLYLDADIICQGTIEPLIKFSF
                     PDDKVAMVVTEGQADWWEKRAHSLGVAGISKGYFNSGFLLINTAKWAAQQVSARAIAM
                     LNEPEVIKKITHPDQDVLNMLLADKLIFADIKYNTQFSLNYQLKESFINPVTNNTIFI
                     HYIGPTKPWHDWAWDYPVSQAFMEAKNASPWKNTALLKPN"
BASE COUNT          189 a          113 c          120 g          181 t
ORIGIN      
        1 agaactggac tcatgcaata tattttcgtt ttgttattgc tgattacttt attagtaagg
       61 tggctaaagt cctttatctg gatgcagata tcatttgtca ggggactatt gagccactaa
      121 ttaaattctc atttcctgac gataaagtcg caatggttgt tacagaaggg caagctgact
      181 ggtgggaaaa acgcgcccat tcgttaggtg ttgcgggaat ttctaaaggt tactttaact
      241 ccggtttttt attgattaat actgccaaat gggcagctca gcaggtttct gcacgagcca
      301 ttgcaatgct aaatgagcca gaagtaatca aaaaaataac acatcctgat caggatgtat
      361 taaatatgtt gctggcagat aaacttattt tcgctgatat taaatataac acccagttta
      421 gcttaaatta tcaactaaaa gaaagcttta taaacccagt aaccaacaat actattttta
      481 tccattatat cgggccaacc aagccctggc atgattgggc ctgggattat cctgtatcac
      541 aagcctttat ggaggcaaaa aatgcttcgc catggaagaa tacagcgttg ctgaaaccga
      601 aca
//
LOCUS       DACADF010000418          596 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.436, whole genome shotgun sequence.
ACCESSION   DACADF010000418 DACADF010000000
VERSION     DACADF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.436"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
BASE COUNT          192 a          123 c          116 g          165 t
ORIGIN      
        1 taagttggag tcattaccta caacgtttat cgacctcaca cgaacaacga tggtttaccg
       61 ccttgcttac atgaagaaaa gtggaaacag gttattccgg tgtcctgatt ttgtgaacca
      121 ctacaataat cctttttcac agacttgtca gcagccagca tttatgttct tttacctgcg
      181 ggaatttatc cgtctgcctg tcgggactac tctgtcatac agaaatcagg taggaataaa
      241 ttgtagtgga aagtcgatgt ttaccggatg actgatgcgc gcttgtacac agacagtgtg
      301 tttcagtaat atggagaata atgaaatgaa taacacagac acattagaaa aaataatcag
      361 acaccaaaaa aacaaagacc ccgcatatcc ctttcgggaa catttgttga tgcagctctg
      421 tattcgcaca aataaaagaa tgcaggataa tatatctgaa tttctggggg tgtatggaat
      481 aaatcactca gcatatatgg ttctcaccac attattcgca gcggagaacc attgtctgtc
      541 accttcagag ataagccaga aacttcagtt taccagaact aatattaccc gcatta
//
LOCUS       DACADF010000419          587 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.437, whole genome shotgun sequence.
ACCESSION   DACADF010000419 DACADF010000000
VERSION     DACADF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.437"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(321..>587)
                     /locus_tag="ISB30_005027"
     CDS             complement(321..>587)
                     /locus_tag="ISB30_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAP1992533.1"
                     /translation="NRDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPA
                     WPAVWLERDDDTGFQQVLERADYLAKEHTKAHCTGERNFGCSRS"
BASE COUNT          135 a          172 c          161 g          119 t
ORIGIN      
        1 cagtacgggc gcccgtcaga cgtccgttgc catcaagagc gataaaggat tttgcgtaca
       61 tagtcatatt cctcagccgc cagacgacac gcaggcaaca gccgtcccgg tacgggcagc
      121 gtggtcaggg tgtgaatggc ggttcagtta aaatctggac tggagtttgg gtaaagcagg
      181 ctggttgctg ttacagcact gtggttgtca cacaatggaa cactgacgta aaaaacagtc
      241 agtgggcgag tggatacatc gttgccgcca gacaacaacc catacttttg gtttgccggg
      301 tattggcgcg ccagtcgtgc tcaactccgg ctacagccaa aattgcgctc accggtgcag
      361 tgagctttgg tatgttcctt cgccagatag tcagcacgtt ccagcacctg ctgaaagcca
      421 gtgtcatcat cgcgttccag ccacaccgcc ggccaggccg gaaaatgcac cagcgtggca
      481 tggcacccga catccactcc cagggcactg caccacgcct gtttaatcat cccggccagt
      541 gatcccggag cacggtaatc accggcacga caccaggtat cccggtt
//
LOCUS       DACADF010000420          587 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.438, whole genome shotgun sequence.
ACCESSION   DACADF010000420 DACADF010000000
VERSION     DACADF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.438"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            47..587
                     /gene="papA"
                     /locus_tag="ISB30_005028"
                     /pseudo
     CDS             47..587
                     /gene="papA"
                     /locus_tag="ISB30_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria major subunit PapA"
BASE COUNT          156 a          100 c          159 g          172 t
ORIGIN      
        1 ccagatatct ctggtgtgtt cagtaatgaa aaagaggttg ttatttatga ttaagtcggt
       61 tattgccggt gcggtagcta tggcagtggt gtcttttggt gtaaatgctg ctctaactac
      121 tccacagggg cagggtagag taacctttaa cggaactgtt gttgatgctc catgcagcat
      181 ttctcagaaa tcagctgatc agtctattga ttttggacag ctttcaaaaa gcttccttca
      241 agcaggaggc gtatccaaac caatgaattt agatattgaa ttggttaatt gtgatatcac
      301 ttcatttaag gggggggagg agtctcggca gcaaaaaaag ggactgtgaa gctggctttt
      361 agtggtccaa gggtttctgg tcataatgag gagttagata ccagcggggg gacaggtact
      421 gcgattgtag ttcaggctgc aggtaaaaac gtttctttcg atggcacaga aggtgatgct
      481 aataccctga aagatggtga taatgtgctg cattatactg cgattgttaa gaagtcgtca
      541 gccaataatg cccaagtcac tgagggtgct ttttctgcag tcgcaac
//
LOCUS       DACADF010000421          586 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.439, whole genome shotgun sequence.
ACCESSION   DACADF010000421 DACADF010000000
VERSION     DACADF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.439"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..225)
                     /locus_tag="ISB30_005029"
     CDS             complement(<1..225)
                     /locus_tag="ISB30_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HAP1992534.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ENTFRRDLIDSIIRDFSNSDTGGPNRRTAIA"
     gene            complement(268..504)
                     /locus_tag="ISB30_005030"
     CDS             complement(268..504)
                     /locus_tag="ISB30_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HAP1992535.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          167 a          124 c          106 g          189 t
ORIGIN      
        1 agcgatcgct gttcttcggt ttggccctcc tgtatcactg ttgctaaaat cacgaattat
       61 gctgtcaatt aaatctcgtc ggaaagtatt ttcctcagcc gatttcatta cgagtaattg
      121 cttaataaga gtctgcaacg aaaaatttat cttcatgcgc agtatctgga gcaccgcctg
      181 gccagtaatt tcatatttaa tactttcatt atcagatgtc ttcataacga cttcatagct
      241 aacctgttaa ataatcaggc tgttgcatta ttcaaaaagc aacccaatta gtgcattata
      301 atgtttctgt tcctcttccc ctgtcgcttg ttccagtcgg caaagaagtt tggcacaaag
      361 cgactttcga ctcagattac gtccatcttt caaaatctca gtcacaattt ccccgagagt
      421 ttcctgctga gtaggcaagt tagcttttgc aaagtaacga gtgattgctc ctgcagaatc
      481 tgaagtcact gaattttgat gcatgctacg cctctgttaa aaatgttaat caatgttaag
      541 tgaggtaaca atagcaaact tgtacgaaat atgtgtacag aatgat
//
LOCUS       DACADF010000422          585 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.440, whole genome shotgun sequence.
ACCESSION   DACADF010000422 DACADF010000000
VERSION     DACADF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.440"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>585
                     /locus_tag="ISB30_005031"
     CDS             <1..>585
                     /locus_tag="ISB30_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAP1992536.1"
                     /translation="CYSELSVQHNLVVQGDFALTQTQMATYEHNFNDSSCVSTNTITP
                     MSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTSFTSGYSVTVTPAASNAKVNVS
                     AGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGGKSWDACVNSYRNALAQNAGVY
                     SFNLTLSYNPITTTCKPDDLLITLESIPVSQLPAT"
BASE COUNT          176 a          111 c          122 g          176 t
ORIGIN      
        1 atgttatagt gagttatctg ttcagcacaa tttggttgtt cagggggatt ttgcacttac
       61 tcaaacacaa atggcgacat atgagcataa ttttaatgat tcgtcatgcg tgagtacaaa
      121 tactatcacc cctatgagcc cgtcggatat tattgttgga ctttataacg ataccattaa
      181 attaaattta cattttgaat ggacaaataa aaacaacatt acattgtcaa ataatcagac
      241 cagtttcacc agtggttatt cggtaacggt gacacctgcg gccagtaatg caaaagtgaa
      301 tgtttctgcg gggggcggtg gttcagtgat gattaatgga gttgcgacat tatccagtgc
      361 ttcatcatcg acacgtggga gtgccgcagt acaatttcta ctgtgtttat taggtggtaa
      421 gtcatgggat gcatgtgtaa atagctacag aaatgcattg gcacaaaatg caggtgtcta
      481 ttcctttaat ctgacattgt catacaaccc gataaccaca acctgcaaac cggacgattt
      541 attaattacg ttagagagta ttcccgtttc acaattacca gccac
//
LOCUS       DACADF010000423          573 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.441, whole genome shotgun sequence.
ACCESSION   DACADF010000423 DACADF010000000
VERSION     DACADF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.441"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
BASE COUNT          176 a          120 c          116 g          161 t
ORIGIN      
        1 atctttacag gaatcagcag aggaaaacat ctattattac catgtaattc atgttgttat
       61 gtcgcaacaa tagtactgca cggggaatta ttacgggcaa acttaggcga aaggaagtac
      121 tgaagtgaca gaataaatca cactttttgt acatatgtac ttttctattg tcttttgttg
      181 tggaaacaaa tagtgtttgt catgccgatg ctaatagaca gatagattcg agggccaaca
      241 gccccatcat catgatggag caacagaggt gagtctaacg tttccgcact tcttatcgat
      301 cggcactaac gatcaatatc gataaactga tctatcaaaa cgattatctt tcttgcaacc
      361 tgcctggtta tccttctctt acattgctcg cagaaaagcc cttttattac ggttagttat
      421 ctagctgaga cttgctacgc ccattttcca tgtaccccaa atcacgaggt cacaaaggcg
      481 cggaaagatg aagagatgtg cacgttgcaa caaaaaaaga agcgtccttg aacagaggct
      541 ctcactcgat aaagaattct gcgacgagtg ctt
//
LOCUS       DACADF010000424          565 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.442, whole genome shotgun sequence.
ACCESSION   DACADF010000424 DACADF010000000
VERSION     DACADF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.442"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
BASE COUNT          150 a          158 c          127 g          130 t
ORIGIN      
        1 taccattttg cagggagccg gtgcgttact gtctggcaca acaaacgcag cgggtcgctc
       61 tgccccgccg attcctgata atccctcccc tgtaacctac tgcaacagta caaatacact
      121 gacgcctgaa acggtaacca gaatcagcac cagcagtaac gtcagtaaac agagcagacg
      181 atcggtcttc cggtaataac cgaaggggtc gacagcgcca caatacgggc aacaaactgt
      241 attatgagta attgcgttac cacagtccct gcacttcttt ttgcgtatca cgatagctcc
      301 ctcgccacgc cttatccgta accggttttt tacattaaaa aaaacaacgc cgggaattca
      361 atcgtcagtc cggagacgac cgttcgggtt atcacagagt gcctgagaca gtgtcctgcc
      421 ggaggtcact tcagggactc tgcgtatttt ttacgacgtg gctattccgt atgaaccata
      481 cggagattta accatgacct acaaatacaa ccccttctgg cagcaacgta ttcgtgagac
      541 ggtgcggcac gcactgaatg ttcat
//
LOCUS       DACADF010000425          565 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.443, whole genome shotgun sequence.
ACCESSION   DACADF010000425 DACADF010000000
VERSION     DACADF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.443"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>565
                     /locus_tag="ISB30_005032"
     CDS             <1..>565
                     /locus_tag="ISB30_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP1992537.1"
                     /translation="SVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFK
                     SEALRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDY
                     DSENKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATS
                     FLVDFLPILRRTLTRTGFVIDHIHYYA"
BASE COUNT          100 a          190 c          175 g          100 t
ORIGIN      
        1 tttcggttgg cctgtgcctc gtgcatgtcg cctgcgacaa gcgcccttgg ctggaaggac
       61 tgaacgtgga aatggattgg cagatgagcg gcaagccctt gctgctctac ctagacaacg
      121 cggccgagtt caagagcgag gccctgcgcc ggggttgcga gcagcatggc atccggctgg
      181 actatcgccc gctgggacag ccgcactatg gcggcatcgt ggaacggatc atcggcacgg
      241 cgatgcagat gattcacgac gaactgccgg gaacgacctt ctccaaccct gaccagcgcg
      301 gcgactacga ttccgaaaac aaggccgccc tgacgctgcg cgagctagag cgctggctca
      361 cattggcggt cggcacctac cacggttcgg tgcacaacgg cctgctccaa ccgccggccg
      421 cgcgctgggc cgaggccgtg gcgcgtgtcg gcgtaccggc cgtcgtcaca cgcgctactt
      481 cgttcctggt cgattttctg ccgatcctcc ggcgcacgct gacccgcacc ggctttgtca
      541 tcgaccacat ccactactac gccga
//
LOCUS       DACADF010000426          564 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.444, whole genome shotgun sequence.
ACCESSION   DACADF010000426 DACADF010000000
VERSION     DACADF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.444"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            89..>564
                     /locus_tag="ISB30_005033"
     CDS             89..>564
                     /locus_tag="ISB30_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAP1992538.1"
                     /translation="MFSKNKITIAAISALLISGTAMAQTSSGSVSATGSFNAATCSVT
                     LPNATASFGTVTNQEVNAAKAYQKIKSVTADQGISVTDCTTPASLTVATTNFQSNGYV
                     YPALNEATGNQQKDFALWMKVGDKGVSSYNAPIELANGTYPIELQMIKISNNYNKN"
BASE COUNT          182 a          116 c          102 g          164 t
ORIGIN      
        1 ctaatttttg aaagttttat tctcacatct ctttgcccat cactatacac ataggaaaaa
       61 tttaagaaaa tcaagattga ggttgaaaat gttttctaag aataaaatca ccatcgcagc
      121 tatttcggct ctgcttattt ctggtaccgc aatggcccag acaagcagtg gttctgtctc
      181 cgctactggt tcttttaatg ccgcgacttg cagtgttacc ctgcctaatg caacagcatc
      241 ttttggcaca gtaacgaatc aagaggtaaa tgctgcaaaa gcgtaccaga aaataaaaag
      301 tgtaaccgca gatcagggga ttagtgttac cgattgtacc actcctgcat cactaacggt
      361 agcaactact aatttccagt ctaatggcta tgtttatcct gctctaaacg aggctaccgg
      421 taatcaacaa aaggattttg cgctttggat gaaagtcggt gataaaggtg tatcttctta
      481 taatgcacca atcgaacttg ctaatggtac atatccaatc gagttgcaaa tgattaaaat
      541 atcaaataac tacaataaaa atca
//
LOCUS       DACADF010000427          564 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.445, whole genome shotgun sequence.
ACCESSION   DACADF010000427 DACADF010000000
VERSION     DACADF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.445"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
BASE COUNT          145 a          159 c          126 g          134 t
ORIGIN      
        1 taccattttg cagggagccg gtgcgttact gtctggcaca acaaacgcag cgggtcgctc
       61 tgccccgccg attcctgata atccctcctc tgtaacctac tgcaacagta caaatacact
      121 gacgcctgaa acggtaacca gaatcagcac cagcagtaac gtcagtaaac agagcagacg
      181 atcggtcttc cggtaataac cgaaggggtc gacagcgcca caatacgggc aacatactgt
      241 attatgagta attgcgttac cacagtccct gcacttcttt ttgcgtatca cgatcgctcc
      301 ctctccacgc cttatccgta acctgttttt tacattaaaa aaacaacgcc gggaattcaa
      361 tcgtcagtcc ggcgatgacc gttcgggtta tcacagagtg cctgagacag tgtcctgccg
      421 gaggtcacct caggggctct gcgcattttt tacgacgtgg ttattccgta tgaaccatac
      481 ggagatttaa ccatgaccta caaatacaac cccttctggc agcaacgtat tcgtgagacg
      541 gtgcggcacg cactgaatgt tcat
//
LOCUS       DACADF010000428          560 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.446, whole genome shotgun sequence.
ACCESSION   DACADF010000428 DACADF010000000
VERSION     DACADF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.446"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(72..545)
                     /locus_tag="ISB30_005034"
     CDS             complement(72..545)
                     /locus_tag="ISB30_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA25"
                     /protein_id="HAP1992539.1"
                     /translation="MKVSLMAARAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGPLPNRKYAVVTRSNWTAANENVVVFPSIDEAMGRLGEITDHVIVAGGGEI
                     YHETIPMASTLHVSTIDVEPEGDVFFPNIPGKFDVVFEQQFTSNINYCYQIWQKG"
BASE COUNT          143 a          137 c          126 g          154 t
ORIGIN      
        1 gctatcaatt gcggcaaatt gcgtagtgag tgaagcgaac gaagcttttt gccgtcaatt
       61 gcatagcttt gttaaccctt ttgccagatt tgatagcaat agttaatgtt tgatgtaaat
      121 tgttgctcaa aaacgacatc aaacttccca ggaatgttcg gaaagaaaac gtctccctct
      181 ggctcaacgt cgattgtcga cacatgcaga gtagaggcca tgggtatcgt ttcatggtag
      241 atttctccac caccggcgac gatgacatgg tcagtgatct cgcctaatct acccatcgct
      301 tcgtcaatcg acgggaaaac cactacgttt tcattagccg ctgtccagtt tgagcgggta
      361 acaaccgcgt atttcctatt gggcagcggc cccatagact caaatgtttt gcgccctacc
      421 aaaagccatt ggttataggt cagtgctttg aaaagaagct gttccccttt ggcagaccaa
      481 ggaatgtcag gaccgcaacc gattacgcca tttttcgctc ttgcagccat caatgatact
      541 ttcaaggtac tcgtcctggg
//
LOCUS       DACADF010000429          552 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.447, whole genome shotgun sequence.
ACCESSION   DACADF010000429 DACADF010000000
VERSION     DACADF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.447"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..>552)
                     /locus_tag="ISB30_005035"
     CDS             complement(<1..>552)
                     /locus_tag="ISB30_005035"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAP1992540.1"
                     /translation="ALKADGIPVSLDSYQPATQAYALSRGVAYLNDIRGFPDAAFYPQ
                     LAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAALTGAGIKRNRLVLDP
                     GMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRALTGRGPGDVGAATLA
                     AELAAAAGGADFIRTHEPRPLRDG"
BASE COUNT          126 a          168 c          174 g           84 t
ORIGIN      
        1 ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc tgcggcggcg
       61 gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc tgtgagcgcg
      121 cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg cagccgcaat
      181 tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag aaaaaacccc
      241 atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag cgccgcgatg
      301 cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc ctcgcgccga
      361 tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga tttcgccaat
      421 tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc cacaccacgc
      481 gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg aatgccatct
      541 gccttgagcg cg
//
LOCUS       DACADF010000430          535 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.448, whole genome shotgun sequence.
ACCESSION   DACADF010000430 DACADF010000000
VERSION     DACADF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.448"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..507)
                     /locus_tag="ISB30_005036"
     CDS             complement(<1..507)
                     /locus_tag="ISB30_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP1992541.1"
                     /translation="MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGT
                     DKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQL
                     KTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
                     GFFLSTSIK"
BASE COUNT          160 a           91 c          121 g          163 t
ORIGIN      
        1 ttttatactt gtagagagga agaatcccgg tgtattcgta gtgctaccaa taccgtaaaa
       61 gcgtccattt aagccagaca tggcgtgttt ataatagggg cgatagctat aattttgccc
      121 tacatagctg ccggggtcct gccagttact agaagcgatg gccttacctt gggtatccag
      181 aataaatatt gctgcggttt tgagttgagt attgaaatgt gcaattcgtt gattgagttc
      241 gctggacgtc atatcgtcag gcgtaattac cgcctgacgg atgatatggt ctgttgagag
      301 catatagggt aaaacataaa attgctcgat ggtggaatat aatgtggact tatacatttc
      361 aagccgcatt ttatcggtac cggacaaggt attaaaacgc aagtgcaaaa aagcgattga
      421 aaccgcgagc atgataagaa aaagtgaaat gcttgtaatg tgaaatcgcc atttgttctt
      481 aaatatataa ttccacgata ttttcattca tcatccattg aatgcattta tattt
//
LOCUS       DACADF010000431          528 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.449, whole genome shotgun sequence.
ACCESSION   DACADF010000431 DACADF010000000
VERSION     DACADF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.449"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
BASE COUNT          159 a           91 c          120 g          158 t
ORIGIN      
        1 taatttattt agaatataaa caaagaaagc tttgcaagct aactcagtga gcttggtgaa
       61 aataagtgtt tacccgccat caggctgagc ataattctca tcatgaaata tgtttcttgg
      121 tttgtggctt gtaactggtc actcctgatg tcgatctgga gaggcttgtt gatgctggtg
      181 ttttcaggat gatgtttcaa ttagtttgtt tgccatatcg cccggcgagt agctgtgatt
      241 gaggagggtt taagtcgtag tgaccaaagc catatttacc aacggatgta gatgaaaaag
      301 cgtcgcctgc gttcccccat atctccagga taaaaaggaa tgtaacaatc tcatgaccta
      361 aactgacgaa ccagccagga ataatcgcta gggacctaag aattagcttg ataatagcca
      421 ctaagaaatt actgcgttcc atgaaatagc cattttgtgg taaatggagt tgactaataa
      481 tgtcatatgt gagacggcta gttgaacgaa tattaaattt tgctgaat
//
LOCUS       DACADF010000432          518 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.450, whole genome shotgun sequence.
ACCESSION   DACADF010000432 DACADF010000000
VERSION     DACADF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.450"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>518
                     /locus_tag="ISB30_005037"
     CDS             <1..>518
                     /locus_tag="ISB30_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP1992542.1"
                     /translation="TPSAVSQSLQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKN
                     LRGLEQTINIVNKSELKKNFIIYGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAE
                     NAEELLVQRKTDLAITLQPVISRSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAE
                     EFTLLISKSAGI"
BASE COUNT          164 a          122 c           86 g          146 t
ORIGIN      
        1 taacaccctc tgctgttagt cagtcgttac aacgtttacg tgcgcaattt aatgacccgt
       61 tattcattcg ctcagggaaa ggtatcgcgc cgactacaac agggttaaac ctgcatcacc
      121 atcttgagaa aaatctcaga ggccttgaac aaacgatcaa tatcgttaat aagtctgaac
      181 taaagaaaaa ttttattatc tacggtcctc agcttatttc ttgtactaat aacaacatgc
      241 taatccgctg tcttagacaa gatacttcaa tcgatataga atgccatgac atactgcttt
      301 cagctgaaaa tgccgaagaa ctgttggttc aacgcaaaac tgacctggca attacgctgc
      361 aaccagtaat tagtcgctct gtcatctgca tgccgttaca cactattcgc aattccctta
      421 tatgtagcaa caaacatcct cgtattactg ataccagtaa ctatgaacaa attatcgccg
      481 aagaattcac cctgttgata tctaaatcag cagggatt
//
LOCUS       DACADF010000433          491 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.452, whole genome shotgun sequence.
ACCESSION   DACADF010000433 DACADF010000000
VERSION     DACADF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.452"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..457
                     /locus_tag="ISB30_005038"
     CDS             <1..457
                     /locus_tag="ISB30_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAP1992543.1"
                     /translation="VTETCREFNGITTTGRMLYRNLKITSALDEIYGGICKKAHAATE
                     LAKGLRLNLFMKGPFDPVEDYTVHEITLGPGCNVPGYAGTTIGYISTLPASQAKRWTN
                     EQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGNDPIIGIEAYPG"
BASE COUNT          159 a           97 c          105 g          130 t
ORIGIN      
        1 tgtgacagaa acatgcagag aattcaatgg tattacaaca acaggacgta tgctctatcg
       61 aaacttgaaa atcacctcag ctttagacga aatatatggt ggtatatgta aaaaggcgca
      121 tgctgccaca gagttagcaa aaggattacg gctaaattta tttatgaaag gccctttcga
      181 tcctgttgag gactatacag ttcatgaaat aacattaggc cctggttgca atgttcctgg
      241 ttatgccgga accacgatag gatatatttc aacccttcca gcttctcagg caaaaagatg
      301 gaccaatgag cagccaagga ttgatatcta tattgatcag ataatgacgg taacaggggt
      361 agcaaattct tccggattcg cccttgccgc attacttaat gctaatattg agttgggtaa
      421 tgacccaata attggtatag aagcctatcc cgggtgatga aaatgcacca ctcaaacgta
      481 tgactctgca a
//
LOCUS       DACADF010000434          484 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.453, whole genome shotgun sequence.
ACCESSION   DACADF010000434 DACADF010000000
VERSION     DACADF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.453"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>484
                     /locus_tag="ISB30_005039"
     rRNA            <1..>484
                     /locus_tag="ISB30_005039"
                     /product="16S ribosomal RNA"
BASE COUNT          119 a          119 c          143 g          103 t
ORIGIN      
        1 gctgtcgtca gctcgtgttg tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta
       61 tcctttgttg ccagcggtcc ggccgggaac tcaaaggaga ctgccagtga taaactggag
      121 gaaggtgggg atgacgtcaa gtcatcatgg cccttacgac cagggctaca cacgtgctac
      181 aatggcgcat acaaagagaa gcgacctcgc gagagcaagc ggacctcata aagtgcgtcg
      241 tagtccggat tggagtctgc aactcgactc catgaagtcg gaatcgctag taatcgtgga
      301 tcagaatgcc acggtgaata cgttcccggg ccttgtacac accgcccgtc acaccatggg
      361 agtgggttgc aaaagaagta ggtagcttaa ccttcgggag ggcgcttacc actttgtgat
      421 tcatgactgg ggtgaagtcg taacaaggta accgtagggg aacctgcggt tggatcacct
      481 cctt
//
LOCUS       DACADF010000435          474 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.455, whole genome shotgun sequence.
ACCESSION   DACADF010000435 DACADF010000000
VERSION     DACADF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.455"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..193)
                     /locus_tag="ISB30_005040"
     CDS             complement(<1..193)
                     /locus_tag="ISB30_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAP1992544.1"
                     /translation="MENNTSLETTDKTNIVTYGENAVGVLACSSPGESRTCVDAVDDE
                     VCDSNSYEVISRADLKMNGG"
     gene            complement(217..474)
                     /locus_tag="ISB30_005041"
     CDS             complement(217..474)
                     /locus_tag="ISB30_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAP1992545.1"
                     /translation="MDDGVTGYINNASVMTSGDGSYGISVDSQNKVLYISDSNIKTSG
                     SVSDKENGGITASAVVSEFGGTIVMNGDNSVETRGHIQRDF"
BASE COUNT          135 a          125 c           65 g          149 t
ORIGIN      
        1 aaccaccatt catttttaaa tcagcacggc taataacttc gtaactgtta gaatcacaaa
       61 cttcatcatc tacagcatcg acacatgttc gagactctcc aggacttgaa catgcaagaa
      121 ctcctactgc attttcccca taggtaacaa tgttcgtttt atctgtggtt tcaagactgg
      181 tgttattttc cacagtccca gaatcattaa cctgacttaa aaatcccgct gaatatgccc
      241 cctcgtctcg actgaattat caccattcat aacgatggtg ccaccaaatt cactgactac
      301 tgcgctggct gttatccctc cattttcttt gtcagatacg cttccagagg tcttaatatt
      361 gctatcgctt atatataata ctttgttttg gctatcaaca gaaataccat aagatccatc
      421 accactagtc atcacactgg cattatttat atatccagtt acaccgtcat ccac
//
LOCUS       DACADF010000436          473 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.456, whole genome shotgun sequence.
ACCESSION   DACADF010000436 DACADF010000000
VERSION     DACADF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.456"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(196..414)
                     /locus_tag="ISB30_005042"
     CDS             complement(196..414)
                     /locus_tag="ISB30_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992546.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQH"
BASE COUNT          166 a           99 c           82 g          126 t
ORIGIN      
        1 ttttgtttaa caaataactc ttcgacatca aaattatcaa cagttgccat agaaattatt
       61 tcactgatat ataaaacaac tgtgataaca ccagggttga caactatttg cacaataatt
      121 cctttccttc aatgatccag gggttcactg ccagcaaccc attcagccgc atactgacag
      181 tgagaaactg aaagattagt gctgatccct gtggctgacg agtctttccc ttaatcgatt
      241 aaccagattg atcgccagcc aaacaagggc attaagcaaa tttatcgtca caagcacttc
      301 tcccagcccg gtgctgggtt ttgcaataag gatgaccagg agagtgatca acataaatgc
      361 aatggaaaag atgatataga gagtgaaata atccagatta ttactattgt tcatgctact
      421 aatggcaatc cagtcaaaac tttcaaacaa ttatagcaaa caaaagaatg aac
//
LOCUS       DACADF010000437          465 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.457, whole genome shotgun sequence.
ACCESSION   DACADF010000437 DACADF010000000
VERSION     DACADF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.457"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>465
                     /locus_tag="ISB30_005043"
     CDS             <1..>465
                     /locus_tag="ISB30_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP1992547.1"
                     /translation="RQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELLQKRFLTKQ
                     KRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAARDLQGVGG
                     EPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLGMVV"
BASE COUNT           87 a          150 c          152 g           76 t
ORIGIN      
        1 cggcaaggca gcggcctcgt gacggatctg gtgcccggcc agtccggtgg aggtaaaggt
       61 aaggggcgct tgccggaacc ggtcgagcgc gtcatccacg agctactgca aaagcggttc
      121 ctgaccaagc agaagcgcag cctagcggcc tttcaccgcg aagtcactca ggtgtgcaag
      181 gctcaaaaac tgcgagtgcc ggcgcgcaat accgtggcct tacggatcgc tagccttgac
      241 ccgcgcaagg tcatccgccg gcgggaaggc caggatgccg ctcgtgacct acaaggtgtg
      301 ggcggcgagc ctcctgccgt gaccgcgccg ctggagcagg tgcagataga ccatacggtc
      361 atcgacctga tcgtggtcga tgaccgcgac cggcaaccta ttggccgccc gtacctgacc
      421 ctcgccatcg acgtgttcac ccgctgcgtg ctcggcatgg tcgtc
//
LOCUS       DACADF010000438          461 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.458, whole genome shotgun sequence.
ACCESSION   DACADF010000438 DACADF010000000
VERSION     DACADF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.458"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            84..275
                     /locus_tag="ISB30_005044"
     CDS             84..275
                     /locus_tag="ISB30_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAP1992548.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          119 a          112 c           88 g          142 t
ORIGIN      
        1 taacgaagaa ttgaccattt tgtcctgttg tgccttaatg taagtaccgt ccacggcgtg
       61 ggacatactt caaggaacct tttgtgagtc aggcaaccag tatgcgaaaa cgacaccgat
      121 ttaacagtcg catgacccgt atcgtactgc tcatcagctt tatcttcttc tttggccgtt
      181 ttatctactc gtccgtcggt gcctggcagc accatcagag caaaaaagaa gctcagcaat
      241 ccacactctc cgtcgaatca ccggtacaac gttagcggtt accttctcca ctctcacaga
      301 acataacggc acctcgctgt cggatgcttt tgctctttga gattatcaaa gcggcagata
      361 ttctttcatc ttaaatttta cgtctttgtt ctgactgcag ttcggctgcg aaataaatac
      421 atcattaata catatgttgt gatgatgtat ttttataaat t
//
LOCUS       DACADF010000439          452 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.459, whole genome shotgun sequence.
ACCESSION   DACADF010000439 DACADF010000000
VERSION     DACADF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.459"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>452
                     /locus_tag="ISB30_005045"
     CDS             <1..>452
                     /locus_tag="ISB30_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP1992549.1"
                     /translation="NKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREGEYL
                     PSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGIVKD
                     FLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNS"
BASE COUNT          152 a           94 c           97 g          109 t
ORIGIN      
        1 acaacaaaaa aaccgtgtta cccgggctaa caacgaccag aaagcccatt gttacaaaaa
       61 aactttcaga aatcgttgcc gaagagcttg aaagaatgat ccgccaggag gagataaggg
      121 aaggtgaata cctgccttct gaacgtgaac ttatggcatt ctttggcgtt ggaagaccat
      181 ctgtcagaga ggctttatca tctctcagac ataaagggct aatccggtat aacaagggag
      241 aaaaagccag tgtctgccgt ccatcaccag aaaccatcat taatgggtta tcaggcattg
      301 ttaaagattt tttatctaca gctgatggca tcaaggattt tgaaagacta cgcatctttt
      361 ttgaaaccaa ccttgtaaga tatgcagcaa aaaatgcaac gcctgaggat attcacaggc
      421 ttgagaaggt gctcagtatc catcataact ca
//
LOCUS       DACADF010000440          451 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.460, whole genome shotgun sequence.
ACCESSION   DACADF010000440 DACADF010000000
VERSION     DACADF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.460"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            10..>451
                     /locus_tag="ISB30_005046"
     CDS             10..>451
                     /locus_tag="ISB30_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAP1992550.1"
                     /translation="MNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFTSTD
                     KAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITSFKHLSSSQDEARYEVRLE
                     PRMALLTRSRQNAIYQNLTVPQIVEKILRERHQMRGQDFVFNLKS"
BASE COUNT          105 a          147 c          114 g           85 t
ORIGIN      
        1 gttcctgttc tgaatttcga ccactcacac cacaagctca aaattcgcgg cctgcaaagt
       61 ccggtggatg tgctgacctt cgaaggccgc gaacaactga gtaccccgtt ccgctacgac
      121 attcagttta ccagtaccga taaagccatc gcgccggagt cggtgctgat gcaggacggc
      181 gcattcagcc tgacggcacc accggtacag ggaatgccgg tgcagactgc cctgcgcacc
      241 ctgcacggcg tcattaccag ctttaaacac ctctcctcgt cacaggacga agcccgttac
      301 gaagtgcgcc ttgaaccccg tatggcgctg cttacccgca gccgccagaa cgccatctac
      361 cagaatctga cggtgccgca aatcgtcgaa aaaatcctgc gcgagcggca ccagatgcgc
      421 ggtcaggact tcgtgtttaa cctcaaaagc g
//
LOCUS       DACADF010000441          449 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.461, whole genome shotgun sequence.
ACCESSION   DACADF010000441 DACADF010000000
VERSION     DACADF010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.461"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
BASE COUNT          134 a           99 c           95 g          121 t
ORIGIN      
        1 tgcagggaat agtcagggga tattccatag aactgaacgt tcatccccca gaatgagaag
       61 ttgaacatgc ctccatagcg aatagtgttc cggactcgaa tcgttgaacg atatcctgca
      121 atttggagca tgaggtgttt ctatcctatg caacagaatc atcattaatc cagatatagg
      181 cctgaaaaac aggcctggca atatcgatac caacaatcct gatcgtgttc atgggatgtt
      241 actctcggat taatgcagtc agtaaaagta tggcactgac tgcattaaga agggacgtcc
      301 atcatatcgt gacacaaacg ctcccaccga catcgccatt gagtcctgca ccgtatcggt
      361 gaacttgtaa ctatcccgta taatcattct atacacattt attggcttga tgagtcaaat
      421 acttcttaat ttcggagaga agtgtgtca
//
LOCUS       DACADF010000442          448 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.462, whole genome shotgun sequence.
ACCESSION   DACADF010000442 DACADF010000000
VERSION     DACADF010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.462"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(112..330)
                     /locus_tag="ISB30_005047"
     CDS             complement(112..330)
                     /locus_tag="ISB30_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HAP1992551.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKSDRQRGEVVGVEEQGKWHEE"
BASE COUNT          125 a          111 c           75 g          137 t
ORIGIN      
        1 gttagtgagc acaaattcaa tctgattgat gccagaataa aggcatctgg catgttgatt
       61 ttatgttcca gaatgacaaa acattgctgt taataagcac gactcttttt gtcattcctc
      121 atgccactta ccctgttctt caacaccaac tacctcaccc ctttgcctgt cactttttgc
      181 cttacgcacc ggaaattgct gacgctccag atattgctct accgcttcgc gtaaaattgc
      241 agagcgcgaa accttacgcc agccccgaag cttgttcagc cgtttaattg cctcttctga
      301 taaatgaaga aaaaccgtat tcatcgccat tgccaccacc ttacatatat tactcattaa
      361 tgtatgtgcg aaaattacgc cgaaaaatca tttaaaaaaa gcggattttt gcatgtcgtt
      421 ttgtttttgc gagactgcat ccaaagat
//
LOCUS       DACADF010000443          418 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.463, whole genome shotgun sequence.
ACCESSION   DACADF010000443 DACADF010000000
VERSION     DACADF010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.463"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>418
                     /locus_tag="ISB30_005048"
     CDS             <1..>418
                     /locus_tag="ISB30_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992552.1"
                     /translation="GVDLVSLKEYPVRIPEEIECQEKLVTILSVIDKKIALNNRINTE
                     LEAMAKTLYDYWFVQFDFPDANGKPYKTSGGKMEYNATLKREIPAGWNDSILGKFIEL
                     DRGVTYSKEDVRTQDDKDTIGILRATNVTGNNVDIDD"
BASE COUNT          136 a           71 c           99 g          112 t
ORIGIN      
        1 ggagttgatc tagtttcttt aaaagaatat ccagtcagga tacctgaaga aattgaatgt
       61 caagaaaaat tggtgactat tcttagtgtt attgataaaa aaatcgccct caacaaccgc
      121 atcaacacgg aactggaagc gatggcgaaa acactgtatg actactggtt tgtacagttt
      181 gatttccctg atgccaacgg aaaaccgtat aaaacctccg gcgggaagat ggagtataac
      241 gccacgctga aacgggaaat tccggcaggt tggaatgact ccattcttgg taagtttatt
      301 gagttagatc gtggcgttac ttatagcaaa gaggatgttc gaactcagga tgataaagat
      361 acgataggca ttcttcgcgc tacaaatgtg acgggaaata atgttgatat tgatgatt
//
LOCUS       DACADF010000444          416 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.465, whole genome shotgun sequence.
ACCESSION   DACADF010000444 DACADF010000000
VERSION     DACADF010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.465"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            43..>416
                     /locus_tag="ISB30_005049"
     CDS             43..>416
                     /locus_tag="ISB30_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HAP1992553.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
BASE COUNT           92 a          111 c          111 g          102 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaa
//
LOCUS       DACADF010000445          415 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.466, whole genome shotgun sequence.
ACCESSION   DACADF010000445 DACADF010000000
VERSION     DACADF010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.466"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..400)
                     /locus_tag="ISB30_005050"
     CDS             complement(<1..400)
                     /locus_tag="ISB30_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HAP1992554.1"
                     /translation="MKIIAKIIYRLTIALMIAGSVQTAAKEKISLLAPYDEWYFLFFY
                     PNALPAQVTYVELLDTDGIYYQFRTLDATAPSSTTIGQWRDNLRVGVLSFNKAKNPPM
                     SIHFCWDSVIDKKVYETWITLAGEVWKLMLT"
BASE COUNT          118 a           85 c           81 g          131 t
ORIGIN      
        1 gtgtgagcat caatttccag acttctcccg ccagcgttat ccaggtttca tacacttttt
       61 tatcaatcac tgaatcccag cagaaatgga tagacatagg gggatttttg gctttattga
      121 atgataacac cccaacccga agattatctc tccactgacc aatagtagtg gaactcggag
      181 cggtagcatc cagcgttctg aactgataat agataccatc agtgtccaat agttctacat
      241 aggtaacttg tgctggtagc gcattagggt agaaaaaaag gaaataccat tcatcatagg
      301 gtgcaagcag tgatattttt tctttcgctg ctgtctggac tgatccggct atcattaacg
      361 caatggttaa tctataaatt attttagcta ttattttcat tgttacttac cagtt
//
LOCUS       DACADF010000446          405 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.467, whole genome shotgun sequence.
ACCESSION   DACADF010000446 DACADF010000000
VERSION     DACADF010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.467"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>405
                     /locus_tag="ISB30_005051"
     CDS             <1..>405
                     /locus_tag="ISB30_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992555.1"
                     /translation="QSNKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGI
                     YLYTDVALPDVVAMTGTGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWQ
                     TLELVFTAGSATVTPKLNGVAGPAFQVIKDSLT"
BASE COUNT          101 a          105 c          116 g           83 t
ORIGIN      
        1 cagagcaata aagcctggtc cctgacgcgc ccggtggatg acgcagtctc cctgctgaca
       61 cggggtggca gactgagctg taagtttcga ctgtcaggcg cactgaccaa caatcagttc
      121 ggtctgggaa tttatctgta taccgatgta gcgttacctg acgtcgtggc gatgaccggg
      181 actggtaacc cgttcctgat gtcgttcttc acccagacca cagacggcaa actgaatctg
      241 atgcatcaca agaaagcagg aaacacaaag ttgggcgagt tcgggaatta cagtaacgac
      301 tggcagacgc tggagctggt gttcaccgcc ggcagtgcca cggttactcc gaaactgaat
      361 ggagtggctg gcccggcatt ccaggtcata aaagacagtc tgaca
//
LOCUS       DACADF010000447          379 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.470, whole genome shotgun sequence.
ACCESSION   DACADF010000447 DACADF010000000
VERSION     DACADF010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.470"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
BASE COUNT          110 a           71 c           77 g          121 t
ORIGIN      
        1 actactttct ttgtatatac tccggataaa ttttcccagc taacttgcct aatcatcact
       61 ctgatgccag aaatgaacag aacgcaaacc atctataact tattgaggat tttgaaaaaa
      121 attgattggg ggcttgagtt atatgatgac tatgctaatt taatacggca catgcaggta
      181 gatttgttgg ttgtggtatc gcaatcagtg ttaacaaggt cgggagtatt cgccctctga
      241 ctgccgtcaa gtcatcttgg cgtcaccgtt aaatgcgtaa gagtacctgc atgtgcatta
      301 acataatcaa taatggaatt tactgttatg tttaaaccta cctatctggc aaggctgcag
      361 gcttgttgta acaaatttg
//
LOCUS       DACADF010000448          369 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.471, whole genome shotgun sequence.
ACCESSION   DACADF010000448 DACADF010000000
VERSION     DACADF010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.471"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            1..369
                     /locus_tag="ISB30_005052"
                     /pseudo
     CDS             1..369
                     /locus_tag="ISB30_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          112 a           94 c           89 g           74 t
ORIGIN      
        1 atgtcggcct ttccacaatg acgcgatggg tgaaacaatt acgtgatgag cggcagggaa
       61 aaacaccaaa agcctcccct attaccccgg aacaaattga aatccgtgag ctcaggaaaa
      121 agctacaacg tattgaaatg gaaaatgaaa tattaaaaaa ggctaccgcg ctcttgatgt
      181 cagactccct gaacagttct cgataatcgg gaaactcagg gcgcgttatc ctgtggccac
      241 tctctgccat gtgttcgggg tccatcgcag cagctacaaa tactggaaaa accgtcctga
      301 aaagccagac ggcagacggg ctgtattacg cagccaggta cttgaactgc atggcatcag
      361 ccacggctc
//
LOCUS       DACADF010000449          358 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.472, whole genome shotgun sequence.
ACCESSION   DACADF010000449 DACADF010000000
VERSION     DACADF010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.472"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..294
                     /locus_tag="ISB30_005053"
     CDS             <1..294
                     /locus_tag="ISB30_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP1992556.1"
                     /translation="KLLTRLNNSDLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSII
                     VVSQLPVENWYKMIGDSTHADAILDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          109 a           78 c           90 g           81 t
ORIGIN      
        1 aaacttttga ccaggctgaa taatagcgat ctgctgcttc tggatgactg ggggctggaa
       61 cctctctcat cagaacagcg tagcgacctg ctggaaatag tggatctgat gtaccaacga
      121 ggctcaatca tcgtagtgag ccagttgccg gtggaaaact ggtacaaaat gatcggagac
      181 tccacacatg cggatgccat cctagatcga ctggttcatg gcagtatcaa gatcgaactt
      241 aaaggagaat caatgcggaa aatacaatct ccgttgaccg aaggagatca gtgaaggtaa
      301 tttaaaaacg gttctgtgaa agtgacacga accgatctcc atcgatgtta ctcaccga
//
LOCUS       DACADF010000450          344 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.473, whole genome shotgun sequence.
ACCESSION   DACADF010000450 DACADF010000000
VERSION     DACADF010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.473"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>344
                     /locus_tag="ISB30_005054"
     CDS             <1..>344
                     /locus_tag="ISB30_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP1992557.1"
                     /translation="GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVE
                     LREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADT
                     GIDIDMHIDAASG"
BASE COUNT           80 a           97 c          103 g           64 t
ORIGIN      
        1 ggcgggatgg cgatgaaatg gcgttggcgc aagcgtatgg aagccgcagg caaacccacg
       61 gataaaccaa acctggtgtg cggtccggta caaatctgct ggcataaatt cgcccgctac
      121 tgggatgtgg agttgcgtga gatccctatg cgccccggtc agttgtttat ggacccgaaa
      181 cgcatgattg aagcctgcga cgaaaacacc atcggcgtgg tgccgacttt cggcgtgacc
      241 tacaccggta actatgagtt cccgcagccg ctgcacgatg cgctggataa attccaggcc
      301 gacaccggta tcgacatcga catgcacatc gacgccgcca gcgg
//
LOCUS       DACADF010000451          340 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.474, whole genome shotgun sequence.
ACCESSION   DACADF010000451 DACADF010000000
VERSION     DACADF010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.474"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..>340)
                     /locus_tag="ISB30_005055"
     CDS             complement(<1..>340)
                     /locus_tag="ISB30_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HAP1992558.1"
                     /translation="VIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYE
                     IASMRLFARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTL
                     VDATIIEAPSS"
BASE COUNT           72 a           86 c          110 g           72 t
ORIGIN      
        1 gagctgggtg cctcaatgat ggtggcatcc accaaagtgc cttgggtcat catgacgcct
       61 gcttcggcca gccagcgatt gatggtcttg aacaattgac gggccagttg atgctgctcg
      121 agcaggtggc ggaaattcat gatggtggtg cgatccggca gggcgctatc cagggataat
      181 cgggcaaaca ggcgcatgga ggcgatttcg tacagggcat cttccatggc accgtcgctc
      241 aggttgtacc aatgctgcat gcagtgaata cgcagcatgg tctccagcgg atagggccgt
      301 cggccattgc ccgccttggg ataaaacggc tcgatgacag
//
LOCUS       DACADF010000452          337 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.475, whole genome shotgun sequence.
ACCESSION   DACADF010000452 DACADF010000000
VERSION     DACADF010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.475"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..245)
                     /locus_tag="ISB30_005056"
     CDS             complement(<1..245)
                     /locus_tag="ISB30_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
                     /protein_id="HAP1992559.1"
                     /translation="MVQRLLFFVLTILVVKRIPSLPLRLLIAAPFVLLTAADMSISLY
                     SWCIFGTTFNDGFAISVLQSDPDEVVKMLGMYIPYLCA"
BASE COUNT          130 a           73 c           64 g           70 t
ORIGIN      
        1 gcacatagat aagggatata cattcccagc attttgacaa cttcatccgg atcactctgc
       61 agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa agatacacca tgaatagagg
      121 ctaatactca tatctgccgc agtcagtaac acaaatggtg cggcaataag caggcgaagc
      181 ggaagggatg gtatacgttt aacaaccagg atggtcagaa cgaagaacag caatcgctga
      241 accatatgcg ctccggaaga agcgactaag ttcgtaatac caaaaataat aacacaaaga
      301 tacaacaacc aatgagattt aaaactttta acaaatt
//
LOCUS       DACADF010000453          332 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.476, whole genome shotgun sequence.
ACCESSION   DACADF010000453 DACADF010000000
VERSION     DACADF010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.476"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..279)
                     /gene="hcp"
                     /locus_tag="ISB30_005057"
     CDS             complement(<1..279)
                     /gene="hcp"
                     /locus_tag="ISB30_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HAP1992560.1"
                     /translation="MGDIVYLRIIGEKQGDISSGCGTYASVGNRWQVGHEDEIFAFAL
                     TNAITSTGNGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRIN"
BASE COUNT          100 a           77 c           55 g          100 t
ORIGIN      
        1 atttatacga tacaaatcga tttcaataaa taaccgctca ttctgattga tggcattaga
       61 cagtagcggt gagcttttat caatgagttt gcaaaattgt agcccctgta gattaacgcc
      121 attaccagta ctggtaatgg cgttggtgag tgcaaaggca aaaatttcat cttcatggcc
      181 aacctgccag cgattaccaa ccgacgcata cgtcccacag cctgaagaaa tatcgccctg
      241 cttctcaccg attattctca ggtaaacaat atcgcccact ttatttcatt cccgtttatc
      301 cttaacttat ttgcattaaa tagaaaactt at
//
LOCUS       DACADF010000454          323 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.477, whole genome shotgun sequence.
ACCESSION   DACADF010000454 DACADF010000000
VERSION     DACADF010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.477"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..153
                     /locus_tag="ISB30_005058"
     CDS             <1..153
                     /locus_tag="ISB30_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C"
                     /protein_id="HAP1992561.1"
                     /translation="YVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDIT
                     RPKAAG"
BASE COUNT           74 a           89 c           85 g           75 t
ORIGIN      
        1 tatgtctggc cgtcagaggc cagtggttcg accatgcgca agcgccgcca gcgggtgcgc
       61 gaggcgttgc cggagctggt cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag
      121 tacgacatca cccggcccaa ggcggcaggc tgaccccccc cactctattg taaacaagac
      181 atttttatct tttatattca atggcttatt ttcctgctaa ttggtaatac catgaaaaat
      241 accatgctca gaaaaggctt aacaatattt tgaaaaattg cctactgagc gctgccgcac
      301 agctccatag gccgctttcc tgg
//
LOCUS       DACADF010000455          317 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.478, whole genome shotgun sequence.
ACCESSION   DACADF010000455 DACADF010000000
VERSION     DACADF010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.478"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>317
                     /locus_tag="ISB30_005059"
     CDS             <1..>317
                     /locus_tag="ISB30_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992562.1"
                     /translation="NRQTFSGGLSGELSGNASTAAKLKTARKISNVAFDGSSDITLKA
                     SHVGAFALGKTGSTVANDKAVGWNWSSGAYNANIGGASTLIIHFYMGDGSCPAAQFRI
                     NYK"
BASE COUNT           98 a           56 c           76 g           87 t
ORIGIN      
        1 aatcgacaaa cattcagcgg tggcctttct ggtgaactat ccggcaatgc ttctacagct
       61 gcaaaattaa aaactgccag gaaaataagc aatgtggctt ttgatggttc ctccgatatc
      121 acattaaaag caagtcatgt tggtgcgttt gccttaggga aaacaggaag caccgttgcg
      181 aatgataaag cagttggatg gaactggagt agcggagcct ataacgcaaa tattggtggt
      241 gcatcaacgt taattattca tttttatatg ggagacggaa gttgtcctgc agctcagttt
      301 cggattaatt ataaaaa
//
LOCUS       DACADF010000456          315 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.480, whole genome shotgun sequence.
ACCESSION   DACADF010000456 DACADF010000000
VERSION     DACADF010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.480"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>315
                     /locus_tag="ISB30_005060"
     CDS             <1..>315
                     /locus_tag="ISB30_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP1992563.1"
                     /translation="KPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYADD
                     GWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLR
                     YVL"
BASE COUNT           79 a           79 c           97 g           60 t
ORIGIN      
        1 aaaccgctgc tgaaatccct ggaaagctgg ctgcgtgaaa agatgaaaac cctgtcgcga
       61 cactcagaac tggcgaaagc gttcgcatac gccctgaacc agtggccggc gctgacgtac
      121 tatgcagatg atggctgggc tgaggcggac aataacatcg ctgaaaatgc gttgcggatg
      181 gtcagtctgg gccgcaaaaa ctacctgttc ttcggttcgg atcatggagg agagcgggga
      241 gcgctgctgt acagcctgat cgggacgtgc aaactgaacg gagtggagcc agaaagctac
      301 ctccgctatg tcctt
//
LOCUS       DACADF010000457          303 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.482, whole genome shotgun sequence.
ACCESSION   DACADF010000457 DACADF010000000
VERSION     DACADF010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.482"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>303
                     /locus_tag="ISB30_005061"
     CDS             <1..>303
                     /locus_tag="ISB30_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP1992564.1"
                     /translation="ESLISAAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMY
                     TRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLA"
BASE COUNT           88 a           77 c           77 g           61 t
ORIGIN      
        1 ggaaagcctt ataagcgcag cgcctgcgct gtcacaacag gcagtagacc aggaatggag
       61 ttatatggac ttcctggagc atctgcttca tgaagaaaaa ctggcacgtc atcaacgtaa
      121 acaggcgatg tatacccgaa tggcagcctt cccggcggtg aaaacgttcg aagagtatga
      181 cttcacattc gccaccggag caccgcagaa gcaactccag tcgttacgct cactcagctt
      241 catagaacgt aatgaaaata tcgtattact ggggccatca ggtgtgggga aaacccatct
      301 ggc
//
LOCUS       DACADF010000458          299 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.483, whole genome shotgun sequence.
ACCESSION   DACADF010000458 DACADF010000000
VERSION     DACADF010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.483"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            8..217
                     /gene="fumD"
                     /locus_tag="ISB30_005062"
     CDS             8..217
                     /gene="fumD"
                     /locus_tag="ISB30_005062"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HAP1992565.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT           99 a           54 c           78 g           68 t
ORIGIN      
        1 ataaagaatg ggaaaccgga caaaagagga tgaactgtac cgagaaatgt gcagagttgt
       61 cggtaaagtt gtgctggaaa tgcgcgatct ggggcaggaa ccaaagcata ttgttatcgc
      121 aggcgtatta cgtacagcat tagccaataa acgcattcag cgcagtgagc tggaaaagca
      181 ggcgatggaa acagtcatta acgcactagt aaaatgacca ttcaggatga ggcggtaagc
      241 ttcatcctga tattgttagt tcagttgcgg tccagttaat cattctaaat aacaactgc
//
LOCUS       DACADF010000459          294 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.484, whole genome shotgun sequence.
ACCESSION   DACADF010000459 DACADF010000000
VERSION     DACADF010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.484"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..>294)
                     /locus_tag="ISB30_005063"
     CDS             complement(<1..>294)
                     /locus_tag="ISB30_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HAP1992566.1"
                     /translation="GITGGNVFVSCLVFVFFYFAILMTVSADMPLVDCVLTLSLLVLL
                     IFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFIST"
BASE COUNT          122 a           72 c           45 g           55 t
ORIGIN      
        1 tgtcgagata aagaaaacag tccccatccc aatggccatt aaaatcggca agaaaaaggt
       61 tacccaccag aaccctttca aagagcaatt aaaactaaat cttacgccgt tgagactagt
      121 catcaaggcc tgataacgca gtccttttgc tgccataaat ataagcaaaa ccaacagtga
      181 caaagtcaaa acacagtcaa caagcggcat atctgctgac actgtcatta agattgcgaa
      241 atagaaaaaa acaaaaacaa gacaactaac aaaaacattc ccaccggtaa tccc
//
LOCUS       DACADF010000460          293 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.485, whole genome shotgun sequence.
ACCESSION   DACADF010000460 DACADF010000000
VERSION     DACADF010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.485"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            65..140
                     /locus_tag="ISB30_005064"
     tRNA            65..140
                     /locus_tag="ISB30_005064"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Glu,seq:ttc)
BASE COUNT           79 a           67 c           70 g           77 t
ORIGIN      
        1 agtgctcaca cagattgtct gatagaaagt gaaaagcaag gcgtcttgcg aagcagactg
       61 atacgtcccc ttcgtctaga ggcccaggac accgcccttt cacggcggta acaggggttc
      121 gaatccccta ggggacgcca cttgctggtt tgtgagtgaa agtcacctgc cttaatatct
      181 caaaactcat cttcgggtga tgtttgagat atttgctctt taaaaatctg gatcaagctg
      241 aaaattgaaa cactgaacaa cgagagttgt tcgtgagtct ctcaaatttt cgc
//
LOCUS       DACADF010000461          292 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.486, whole genome shotgun sequence.
ACCESSION   DACADF010000461 DACADF010000000
VERSION     DACADF010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.486"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..>292)
                     /locus_tag="ISB30_005065"
     CDS             complement(<1..>292)
                     /locus_tag="ISB30_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAP1992567.1"
                     /translation="PSDTIFCRHPSLQYALDSLRNGKGKVNLIKHYSSIESIQQHVPL
                     VRDAEFRALLRHPLARSRVIASQDFGFALDIFFCRMMANNVSHMSAILYIDNH"
BASE COUNT           92 a           54 c           68 g           78 t
ORIGIN      
        1 tatgattgtc tatatataaa atcgcggaca tatgactgac attgtttgcc atcattcgac
       61 agaagaaaat atctaaagca aagccaaaat cctgactcgc gataacgcga ctccttgcaa
      121 gaggatggcg aagtaatgct ctgaattccg cgtcccggac taagggaaca tgctgctgta
      181 tggattcaat ggaggagtaa tgtttaatca ggttgacttt gccttttcca tttctcaatg
      241 aatcaagggc gtattgtaat gacggatggc gacaaaaaat agtgtcagaa gg
//
LOCUS       DACADF010000462          283 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.488, whole genome shotgun sequence.
ACCESSION   DACADF010000462 DACADF010000000
VERSION     DACADF010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.488"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>283
                     /locus_tag="ISB30_005066"
     CDS             <1..>283
                     /locus_tag="ISB30_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
                     /protein_id="HAP1992568.1"
                     /translation="PGIQTQWMSAGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQT
                     DLDGNRARNDQHQFDGYVTYKINDSWLFAWQTTVYTKVNNFKYGNHKKTA"
BASE COUNT           92 a           53 c           63 g           75 t
ORIGIN      
        1 tcccagggat tcagacacaa tggatgtcag caggaagtca gtataaccct tatctgaaaa
       61 tttcgtatca ggcaacgcca gatcttaatc tggccttacg ctatcgctat gactggaaag
      121 ctttccgaca gacggattta gatggcaaca gggcgcgtaa tgatcagcat cagtttgatg
      181 gttatgtgac atacaaaatt aatgattcat ggttgtttgc atggcagacc acggtatata
      241 cgaaggttaa taatttcaaa tatgggaatc ataaaaaaac agc
//
LOCUS       DACADF010000463          271 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.490, whole genome shotgun sequence.
ACCESSION   DACADF010000463 DACADF010000000
VERSION     DACADF010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.490"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>271
                     /locus_tag="ISB30_005067"
     CDS             <1..>271
                     /locus_tag="ISB30_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992569.1"
                     /translation="GKYKNYIRDEIKYWRYTSYKGGEFPEGFTDEKFSSAIYNGRIFT
                     MKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARAEQPGACPTT"
BASE COUNT           85 a           57 c           66 g           63 t
ORIGIN      
        1 cgggaaatat aaaaattaca tccgggatga aataaaatac tggcgatata catcatacaa
       61 ggggggggaa tttccggaag gtttcactga tgagaaattt tccagcgcca tttacaacgg
      121 aagaatattt acaatgaaac gtttacatac cctgatgtta tttctggcgg ttctgtttac
      181 tggctttaac gtggaagcag cgagcgtgaa acaagcgctc agctgcgacc caaacgcccg
      241 ggctgaacaa cctggagcgt gtccaacaac g
//
LOCUS       DACADF010000464          267 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.491, whole genome shotgun sequence.
ACCESSION   DACADF010000464 DACADF010000000
VERSION     DACADF010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.491"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
BASE COUNT           69 a           58 c           75 g           65 t
ORIGIN      
        1 tgaacaaact ttacgatcgc gtgccggctt ccgtctggaa atatgtcacc tgaatcttga
       61 atccgcgcta cgatgactcc ggtgttatct gtgcaccgga agatcgggaa cattaagcag
      121 tataacggca acaaagaatt cgaaatcaaa agggaatggt gtacagaggg gcaggcgttc
      181 aggcattacc ggcctgtatc ttctgaaggg gactcgtatt tgtcggggct gagtattccg
      241 ctcgattcga tcacggattg gcggaat
//
LOCUS       DACADF010000465          266 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.492, whole genome shotgun sequence.
ACCESSION   DACADF010000465 DACADF010000000
VERSION     DACADF010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.492"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>266
                     /locus_tag="ISB30_005068"
     CDS             <1..>266
                     /locus_tag="ISB30_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP1992570.1"
                     /translation="TMATQRGYQMGRWLAGRLMKELGLVSCQQPTYRYKRGGHEHVAI
                     PNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPV"
BASE COUNT           60 a           67 c           81 g           58 t
ORIGIN      
        1 acaatggcaa cccagagagg ctaccagatg gggcgctggc ttgctggcag actcatgaaa
       61 gagctggggc tggtcagctg tcagcagccg acttaccggt ataaacgtgg tggtcatgaa
      121 catgttgcta tccctaacta ccttgaacgg cagttcgccg tgaccgagcc aaatcaggtg
      181 tggtgcggtg atgtgaccta tatctggacg ggtaagcgct gggcgtacct cgccgttgtt
      241 ctcgacctgt tcgcaagaaa accagt
//
LOCUS       DACADF010000466          264 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.494, whole genome shotgun sequence.
ACCESSION   DACADF010000466 DACADF010000000
VERSION     DACADF010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.494"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..173)
                     /locus_tag="ISB30_005069"
     CDS             complement(<1..173)
                     /locus_tag="ISB30_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP1992571.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNAR"
BASE COUNT           87 a           51 c           53 g           73 t
ORIGIN      
        1 tgacgagcgt tgccatcaag atataattca tcattgataa tctgaaatgc aacatcatcg
       61 cgcatttcgt gcagcggaaa tcgttttgac tccgcgatag tagaaatggc ctttgcacca
      121 aaacgtgaat cgagtagttc tgagcggaaa tccgttaaca gcttctggtc catttcgaac
      181 tccttaaatt tatttgaagg caataaaaaa gtaggattta tccgcaatgg aagcaaggca
      241 ttacaggctt aatttaaata acaa
//
LOCUS       DACADF010000467          263 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.495, whole genome shotgun sequence.
ACCESSION   DACADF010000467 DACADF010000000
VERSION     DACADF010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.495"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>263
                     /gene="rfaJ"
                     /locus_tag="ISB30_005070"
     CDS             <1..>263
                     /gene="rfaJ"
                     /locus_tag="ISB30_005070"
                     /EC_number="2.4.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide glucosyltransferase RfaJ"
                     /protein_id="HAP1992572.1"
                     /translation="SRAMYFRLFAFQLLGLTLDRLLYLDADVVCKGDISQLLHLDLNG
                     AVAAVVKDVDPMQEKAASRLSDPELLGQYFNSGVVYLDLKKWA"
BASE COUNT           68 a           43 c           60 g           92 t
ORIGIN      
        1 tcaagagcaa tgtacttccg tttatttgca tttcaattat tgggtttaac acttgatcgt
       61 ttgctttatc ttgatgcaga tgttgtttgt aaaggcgata ttagccaact attgcatctg
      121 gatttaaacg gcgcggttgc tgctgttgtt aaagatgttg acccaatgca ggaaaaggcg
      181 gcatccaggt tgtctgatcc agaattactc gggcagtact ttaattccgg tgtcgtttat
      241 ttagatttaa aaaaatgggc taa
//
LOCUS       DACADF010000468          249 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.497, whole genome shotgun sequence.
ACCESSION   DACADF010000468 DACADF010000000
VERSION     DACADF010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.497"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
BASE COUNT           80 a           51 c           54 g           64 t
ORIGIN      
        1 aaatcaagag aaattgttat agcagtgata cgagacaaca aaacggaatg attgccagcc
       61 gttagaaaac accggacact ctcgtgtaaa aagctaccct gtctatggat atagcgatat
      121 cgactcatca agcgtggact gaatgatatt cagctccata tgggcggcaa cctggaggat
      181 aaacatgatt tcgccgttgg aatcaaggtt gttctgcaat atctaccaga ctgcttcaat
      241 ttatctgtt
//
LOCUS       DACADF010000469          232 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.498, whole genome shotgun sequence.
ACCESSION   DACADF010000469 DACADF010000000
VERSION     DACADF010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.498"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            48..124
                     /locus_tag="ISB30_005071"
     tRNA            48..124
                     /locus_tag="ISB30_005071"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Ile,seq:gat)
BASE COUNT           57 a           53 c           65 g           57 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaatg agcagtaaaa cctctacagg cttgtagctc
       61 aggtggttag agcgcacccc tgataagggt gaggtcggtg gttcaagtcc actcaggcct
      121 accaaatttg caccgcaaat ttgaagaggt tttaactaca tgttatgggg ctatagctca
      181 gctgggagag cgcctgcttt gcacgcagga ggtctgcggt tcgatcccgc at
//
LOCUS       DACADF010000470          225 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.499, whole genome shotgun sequence.
ACCESSION   DACADF010000470 DACADF010000000
VERSION     DACADF010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.499"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..>225
                     /locus_tag="ISB30_005072"
     CDS             <1..>225
                     /locus_tag="ISB30_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HAP1992573.1"
                     /translation="YYQFRTLASTNASPGSVGQWSEKVSGMSSVFNKAKNPPMSIHFC
                     WDSVIDKKVYETWITLAGDVWKLMLTPYSPP"
BASE COUNT           70 a           45 c           51 g           59 t
ORIGIN      
        1 ctattatcag ttcagaactc tggcgagtac taatgccagt ccaggaagtg ttggacaatg
       61 gagcgagaaa gtatcaggga tgtcgtcagt ttttaataaa gccaaaaacc caccaatgtc
      121 tatccatttt tgctgggatt cagtgattga taaaaaagtg tatgaaacct ggataacact
      181 ggcgggagac gtctggaaat tgatgctcac accttattca ccacc
//
LOCUS       DACADF010000471          206 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.denovo.500, whole genome shotgun sequence.
ACCESSION   DACADF010000471 DACADF010000000
VERSION     DACADF010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.denovo.500"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..>206)
                     /locus_tag="ISB30_005073"
     CDS             complement(<1..>206)
                     /locus_tag="ISB30_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992574.1"
                     /translation="CHLCGSALRYHPQYDTELPWFEHTDDRLTEHGQQCPYVRPERRE
                     IQLIKRLQQFVPDALPVVRKASWH"
BASE COUNT           43 a           52 c           58 g           53 t
ORIGIN      
        1 agtgccagct ggctttacgc accacgggta aggcatccgg tacgaattgc tgcagacgtt
       61 taatcaactg tatttctctg cgctccggcc tgacataagg gcactgttga ccgtgctctg
      121 tcagcctgtc gtcagtgtgt tcaaaccagg gaagttcagt gtcgtattgc ggatggtatc
      181 tgagtgcact gccacacaag tggcag
//
LOCUS       DACADF010000472        21954 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.076, whole genome shotgun sequence.
ACCESSION   DACADF010000472 DACADF010000000
VERSION     DACADF010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.076"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ISB30_005074"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ISB30_005074"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAP1992575.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ISB30_005075"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ISB30_005075"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAP1992576.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="ISB30_005076"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="ISB30_005076"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAP1992577.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVDLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ISB30_005077"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ISB30_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAP1992578.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPANANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6384..6698)
                     /locus_tag="ISB30_005078"
     CDS             complement(6384..6698)
                     /locus_tag="ISB30_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HAP1992579.1"
                     /translation="MSARMSVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6796..8298)
                     /gene="yjeM"
                     /locus_tag="ISB30_005079"
     CDS             complement(6796..8298)
                     /gene="yjeM"
                     /locus_tag="ISB30_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAP1992580.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAISFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVVKNPQWVAE"
     gene            complement(8517..9494)
                     /gene="epmA"
                     /locus_tag="ISB30_005080"
     CDS             complement(8517..9494)
                     /gene="epmA"
                     /locus_tag="ISB30_005080"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAP1992581.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9819..11627
                     /gene="frdA"
                     /locus_tag="ISB30_005081"
     CDS             9819..11627
                     /gene="frdA"
                     /locus_tag="ISB30_005081"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAP1992582.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11620..12354
                     /locus_tag="ISB30_005082"
     CDS             11620..12354
                     /locus_tag="ISB30_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAP1992583.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12365..12760
                     /gene="frdC"
                     /locus_tag="ISB30_005083"
     CDS             12365..12760
                     /gene="frdC"
                     /locus_tag="ISB30_005083"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAP1992584.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12771..13130
                     /gene="frdD"
                     /locus_tag="ISB30_005084"
     CDS             12771..13130
                     /gene="frdD"
                     /locus_tag="ISB30_005084"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAP1992585.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            13193..14326
                     /gene="blaEC"
                     /locus_tag="ISB30_005085"
     CDS             13193..14326
                     /gene="blaEC"
                     /locus_tag="ISB30_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAP1992586.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQRIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            14415..14948
                     /locus_tag="ISB30_005086"
     CDS             14415..14948
                     /locus_tag="ISB30_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAP1992587.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14945..15262)
                     /locus_tag="ISB30_005087"
     CDS             complement(14945..15262)
                     /locus_tag="ISB30_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAP1992588.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15438..15584)
                     /gene="ecnB"
                     /locus_tag="ISB30_005088"
     CDS             complement(15438..15584)
                     /gene="ecnB"
                     /locus_tag="ISB30_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAP1992589.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15695..15820)
                     /gene="ecnA"
                     /locus_tag="ISB30_005089"
     CDS             complement(15695..15820)
                     /gene="ecnA"
                     /locus_tag="ISB30_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAP1992590.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15872..16438)
                     /gene="efp"
                     /locus_tag="ISB30_005090"
     CDS             complement(15872..16438)
                     /gene="efp"
                     /locus_tag="ISB30_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP1992591.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16480..17508
                     /gene="epmB"
                     /locus_tag="ISB30_005091"
     CDS             16480..17508
                     /gene="epmB"
                     /locus_tag="ISB30_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAP1992592.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17743..18612
                     /locus_tag="ISB30_005092"
     CDS             17743..18612
                     /locus_tag="ISB30_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992593.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDDGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(18662..19015)
                     /locus_tag="ISB30_005093"
     CDS             complement(18662..19015)
                     /locus_tag="ISB30_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAP1992594.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(19153..20799)
                     /gene="groL"
                     /locus_tag="ISB30_005094"
     CDS             complement(19153..20799)
                     /gene="groL"
                     /locus_tag="ISB30_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP1992595.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(20843..21136)
                     /locus_tag="ISB30_005095"
     CDS             complement(20843..21136)
                     /locus_tag="ISB30_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP1992596.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            21412..>21954
                     /gene="yjeH"
                     /locus_tag="ISB30_005096"
     CDS             21412..>21954
                     /gene="yjeH"
                     /locus_tag="ISB30_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAP1992597.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNI"
BASE COUNT         5437 a         5783 c         5545 g         5189 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 ggcgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccaactcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaagccgtca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctaacatc gagatagcgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg gtcctgaccg atgctgttac
      481 cacaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacacgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgttgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcagtgtt gacgcgcccg gatttctacg acggtgtgaa acctattgct gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gagttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagtcata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgttggcaaa tccagcctgc
     1561 tgaacgcgtt gctgggtctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggcttggtca gcacaccacg accgccgcac gactgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc cccaggtgtg cgtgagtttg gtctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgatga agtacgccca atcgataccg
     2281 atccgaatgt cctggttatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggatctc ttccgtaacg gtacgtttgt gaccacttac ctctccccac
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgaaatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccgatggcg cagattctgg
     2641 tcggtgcgac gattgtaggc agcattgaga cggtatgggc gggcaccatt acgccgccgc
     2701 gcgaaggcat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactaggctc taccgttatc aacctgtttg
     2821 caccaggtaa agtggacctg gttgagcaac tggagagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga tgctgaacca gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacctg cgcagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattacccg aaactttccg ccactctgcg cgcgcagtta aacaacatgc
     3301 gtgacgagcc acgcagcgtg tcgccgggaa tgtctaacga tgcgctgaat caggaaattc
     3361 tccagatcag cagtcagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcc ctgaatcaac tgccgcaaca gcaaaccgat gcccgccgtc
     3481 agttgaatga aatcgagcgc cgcctgggga cgcttaccgg caataccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgtctgaa agcgctcgtt gatgaactgg
     3601 agctggcgca gctatctgcc aataaccgcc aggaattagc gcgcttgcgc tcagagctgg
     3661 ccgaaaaaga gagccagcag ctggatgcat atttgcaagc actacgcaac cagttgaata
     3721 gtcaacgcca actggaagcg gaacgggcgc tggaaagtac cgagcaactg gcggaaagta
     3781 gcgccgattt gccaaaagat atcgtcgccc agtttaaaat taaccgcgaa ctatcggcgg
     3841 cactgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcatctaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggcta ccggaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttacg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgctga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tgctggagct gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgca ctgaaagagg tgaacgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagtaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgcctgt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggtgat ggcgtaacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgctcgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgttactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgcga attttccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagaag gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcacctctgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc gcaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgaccgggc aaagcatcgc cgggcagaga tgttagcgca gcgtgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcact tccacggtac agggcgtaga aagcctggag ccgattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattctgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctaat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttaccggt agtgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg caaagaaatt atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccaatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcgccggaa gtcttcctgg
     6121 tggatctgca gcaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagattcacc agctgattct ggctggcttc cacgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctgaat ggtaaacaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taaggtcttt
     6361 gtggccggat gaacccatcc ggctcacaag caaaactgat agcgcagaaa aaactgccac
     6421 aaccagtagc cgaaaatagc cagcgcggtg gcgtacataa accagccgcg tttttttcgc
     6481 cacaaagcga accagagaaa agggaaaaag agcggtaacg tcattatcac accgacatcg
     6541 cgagtatcgt catcttcgag atacatcaaa acatcacaga cgttgttaat cccgcgtcca
     6601 tcaatcatcc actctttgtc cagcgcggag gtaatggtta tccagaggaa cgccacaatg
     6661 aaccagatgc agcacaagac agacatgcgc gcactcatga ggtaacccat agtttaattt
     6721 cgctatagcg catacgaaat ctcactaatt ccctgtcatt acgccctatc gtcaggccga
     6781 cgatagggca tttgattact cagccaccca ctgcggattt tttaccactc tgctgcaata
     6841 gttctggtaa atcgccatcg ctaacagcga gaagaagaca gggccgccaa tcatccacaa
     6901 tgtgctgtcc cagtctccgg cttcaaccac aggttgaatg atggtgaaga cgttcgcaaa
     6961 tgtcaccacc agtacgacaa ccactgtcgc aatcattgcc gacatacgcg ttttgaaaat
     7021 cacaaacggt ctgtcgagat cctgacgtgc tttaaagaac gggaaagcca gcgcgaggaa
     7081 caggtaagga agcgtcatag acacgttcgc catcagcgtc agcttgttaa agaacgccga
     7141 tgcggtaccg ccaccaaacg aaaccagcag gatgaagaca gtaaccaacc cacactgcat
     7201 ccacatggcg atagacggca tccccatcgc attcaggcgc gtcatcggtt ccggccacaa
     7261 tgctttcggc gtcccctgga tgatggcttt cagcggtgaa tagcaaagcg taaagaacgc
     7321 accggtataa gcgaggaaca tcgacagtcc ggtaatacgc gcaaaccata cgcccagcga
     7381 caatgacgct tcaggtgaca aatgcagtgc gttacccagc gtcaccccga ggctcttcat
     7441 cagcacatag gtaatattgc cgaggttaac ggaaccatta cttaatacct gctgccagtt
     7501 tgtgctgacg ccccataaaa atattgccag cgaataaccg attgaaataa caatagcggc
     7561 aaaaacaata cctttggcaa agttcttttc tggattttcc gttttatcaa ccagaccacc
     7621 aaccgcttca attccgccat aggcaaaaat ggcaaatacc acaaacgata gcattgccag
     7681 accggactga taacctgggt tcggtgatgc aaggaaatta atatcctgcg cgaaatgccc
     7741 accatttaat aacaaaatag taatgcttac taacagcaat actaaattca gacacattac
     7801 tgcaataccg cccaccgcag taatgcgggc aattttatta atcccttttg aagcaacgac
     7861 ggtgaccaga atcatccatg ccactgccag cagaccaacc acctgcgtag gctccagtcc
     7921 ggcaatatgc cagtgctggg tcatgtcgct accgtagagg aatgttgaga acggtaccca
     7981 aactttcgcg gaggtactca ccatccagat gatataagag gaaaaccaca taaacgtacc
     8041 aatgaaggca aaacgtggtc cgacactatt attcatccag gaatagatac cgccttcttc
     8101 tttgcgataa gcagctccca tttcagccat cattaaggcg aatggaataa agaataacaa
     8161 tgcagaaaat atataaaagg aaatcgcact ataacccatt aagtaataag ccgatgggct
     8221 attggcaaat ccaaatacag aagtaaagat catcaatatg agtcctatca gactcatctt
     8281 ttttatcgag tgaggcatga aaccatcctt catttcagcg cgtgtataaa acgcgcccac
     8341 tgaactgcac tgcccaacat tgagcgggca gcgtaaaaac caatacagaa ttgatacctg
     8401 gatgatatca aatatccagt cttaaattgt ggcctttagc ggaaaaatga aaacatatgc
     8461 tacagaatgg cgcttatcac gccattcttc gctgttaatt cagtaatttt tcagaattat
     8521 gcccggtcaa cgctaaaggc gatgacttca gccagtgtct ccgcgcccag cgccaacatc
     8581 accagacgat caacacctaa tgccacgccg gaacagtcag gcataccgac tttcaaggct
     8641 tcaatcagat tctggtcaat ggggtgctgc ggcaaaccgc gcgccgcgcg cttacggtta
     8701 tcttgttcaa agcgttgttg ctgctcacgg gcatccgtca attcatggaa accattcgcc
     8761 agctcaatac ctttataata aacctcaaag cgttcagcga cccgatgatc ttcggtactg
     8821 atttgcgcca gtgatgcctg gctggctgga aagtggtaca caaaggtcgg tttttctttg
     8881 ccaatatttg gctctacgcc aaaggtaaac agcaattgta gaagcgtgtc gcggtcttct
     8941 tcagtatcgg caacgttgct caaatccagt ttcgctgcca cttcccgcag ttgcgttttg
     9001 tcggcagaga gtgggtcaat ttccagataa cgcaagaaag cttgttgata agaaaggctt
     9061 tctgctgccg gacagtccag cacctgttgc aagagatcgt ccacctcgtt catcaaccgg
     9121 tacatatcat agtgcggtcg ataccactca agcatagtga actcagggtt gtgataacgc
     9181 cccatctctt cattacggaa gctgcggcac agctggaata ccggcccaca accggcaacc
     9241 agcaggcgct tcatatggta ttccgggctg gtcattaacc agagattcat cccctgcgaa
     9301 tgcccggggc caacgaaacg tgtctcaaac gggaccaaat gaatatcggt taccgtcgcc
     9361 tggctcatac aaggcgtctc cacctccagc actccacgat cggcaaagaa acgacggatc
     9421 tccgccataa tcgccgcgcg ttttaataag ttaggaatgg atgcgctcgg ctgccaggat
     9481 gccgtttcgc tcatagttaa atctccagtt tttgacaagg gcacgaagtc tactcgcaac
     9541 gcgacggcga gacaaatttt acgcaggaat aacacaacgg tgggcagtga ctaaaaaaag
     9601 cacgatctga tggtttagta attaaattaa tcatcttcag tgataattta gccctcttgc
     9661 gcactaaaaa aatcgatctc gtcaaatttc agacttatcc atcagactat actgttgtac
     9721 ctataaagga gcagtggaat agcgttcgca gaccgtaact ttcaggcact taccctgaag
     9781 tacggggctg tgggataaaa acaatctgga ggaatgtcgt gcaaaccttt caagccgatc
     9841 ttgccattgt aggcgccggt ggcgcgggat tacgtgctgc aattgctgcc gcgcaggcaa
     9901 atccaaatgc aaaaatcgca ctaatctcaa aagtataccc gatgcgtagc cataccgttg
     9961 ctgcagaagg gggctccgcc gctgtcgcgc aggatcatga cagcttcgaa tatcactttc
    10021 acgatacagt agcgggtggc gactggttgt gtgagcagga tgtcgtggat tatttcgtcc
    10081 accactgccc aaccgaaatg acccaactgg aactgtgggg gtgcccatgg agccgtcgcc
    10141 cggatggtag cgtcaacgta cgtcgcttcg gcggcatgaa aatcgagcgc acctggttcg
    10201 ccgccgataa gaccggcttc catatgctgc acacgctgtt ccagacctct ctgcaattcc
    10261 cgcagatcca gcgtttcgac gaacatttcg tgctggatat tctagttgat gatggtcatg
    10321 ttcgcggcct ggtagcaatg aacatgatgg aaggcacgct ggtgcagatc cgtgctaacg
    10381 cggtcgttat ggctaccggc ggtgcaggtc gcgtttatcg ttacaacacc aacggcggca
    10441 tcgttaccgg tgacggtatg ggtatggcgc taagccacgg cgttccgctg cgtgacatgg
    10501 aattcgttca gtatcaccca accggtctac caggttccgg tatcctgatg accgaaggct
    10561 gccgcggtga aggcggtatt ctggtcaaca aaaatggcta ccgttatctg caagattacg
    10621 gcatgggccc ggaaactccg ctgggcgagc cgaaaaacaa atatatggaa ctgggtccac
    10681 gcgacaaagt ttctcaggcc ttctggcacg aatggcgtaa aggcaacacc atctccacgc
    10741 cgcgtggcga tgtggtttat ctcgacctgc gtcaccttgg cgagaaaaaa ctgcatgaac
    10801 gtttgccgtt catctgcgaa ctggcgaaag cgtacgttgg cgtcgatccg gttaaagaac
    10861 cgattccggt acgtccgacc gcacactaca ccatgggcgg tatcgaaacc gatcagaact
    10921 gtgaaacccg cattaaaggt ctgttcgccg tgggtgaatg ttcctctgtt ggtctgcacg
    10981 gtgcgaaccg tctgggctcc aactcgctgg cggaactggt ggtcttcggt cgtctggccg
    11041 gtgaacaagc gacagagcgt gcagcaactg ccggtaatgg caacgaagcg gcaattgaag
    11101 cgcaggcagc tggcgttgaa caacgtctga aagatctggt taaccaggat ggcggcgaaa
    11161 actgggctaa gatccgcgac gaaatgggca tggccatgga agaaggctgc ggtatctacc
    11221 gtacgccgga actgatgcag aaaaccatcg acaagctggc agagctgcag gaacgcttca
    11281 agcgcgtgcg catcaccgac acttccagcg tgttcaacac cgacctgctc tacaccattg
    11341 agctgggcca cggtctgaac gttgctgaat gtatggcgca ctccgcaatg gcacgtaaag
    11401 agtcccgcgg cgcgcaccag cgtctggacg aaggttgcac cgagcgtgac gacgtcaact
    11461 tcctcaaaca caccctcgcc ttccgcgatg ctgatggcac gactcgcctg gagtacagcg
    11521 acgtgaagat tactacgctg ccgccagcta aacgtgttta cggtggcgaa gcggatgcag
    11581 ccgataaggc ggaagcagcc aataagaagg agaaggcgaa tggctgagat gaaaaacctg
    11641 aaaattgagg tggtgcgcta taacccggaa gtcgataccg caccgcatag cgcattctat
    11701 gaagtgcctt atgacgcaac tacctcatta ctggatgcgc tgggctacat caaagacaac
    11761 ctggcaccgg acctgagcta ccgctggtcc tgccgtatgg cgatttgtgg ctcctgcggc
    11821 atgatggtta acaacgtgcc aaaactggca tgtaaaacct tcctgcgtga ttacaccgac
    11881 ggtatgaagg ttgaagcgtt agctaacttc ccgattgaac gcgatctggt ggtcgatatg
    11941 acccacttca tcgaaagtct ggaagcgatc aaaccgtaca tcatcggcaa ctcccgcacc
    12001 gcggatcagg gtactaacat ccagaccccg gcgcagatgg cgaagtatca ccagttctcc
    12061 ggttgcatca actgtggtct gtgctacgcc gcgtgcccgc agtttggcct gaacccagag
    12121 ttcatcggtc cggctgccat tacgctggcg catcgttata acgaagatag ccgcgaccac
    12181 ggtaagaagg agcgtatggc gcagttgaac agccagaacg gcgtatggag ctgtactttc
    12241 gtgggctact gctccgaagt ctgcccgaaa cacgtcgatc cggctgcggc cattcagcag
    12301 ggcaaagtag aaagttcgaa agactttctt atcgcgaccc tgaaaccacg ctaaggagtg
    12361 caacatgacg actaaacgta aaccgtatgt acggccaatg acgtccacct ggtggaaaaa
    12421 attgccgttt tatcgctttt acatgctgcg cgaaggcacg gcagttccgg ctgtgtggtt
    12481 cagcattgaa ctgattttcg ggctgtttgc cctgaaaaat ggcccggaag cctgggcggg
    12541 attcgtcgac tttttacaaa acccggttat cgtgatcatt aacctgatca ctctggcggc
    12601 agccctactg cacaccaaaa cctggtttga gctggcacca aaagcggcca atatcattgt
    12661 aaaagacgaa aaaatgggac cagagccaat tatcaaaagt ctctgggcgg taactgtggt
    12721 tgccaccatc gtaatcctgt ttgttgccct gtactggtaa ggagcctgag atgattaatc
    12781 caaatccaaa gcgttctgat gaaccggtat tctggggcct cttcggggcc ggtggtatgt
    12841 ggagcgccat cattgcgccg gtgatgatcc tactggttgg tattctgcta ccgctagggc
    12901 tgtttccagg tgatgcgctg agctacgagc gcgttctggc gttcgcgcag agcttcattg
    12961 gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg gtgtggttta caccgaatgc
    13021 accacgcgat gcacgatctg aaaatccacg tacctgcggg taaatgggtt ttctacggtc
    13081 tggctgctat cctgacagtt gtcacactga ttggtgtcgt tacaatctaa cgcatcgcca
    13141 atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg aaaccagacc ctatgttcaa
    13201 aacgacgctc tgcaccttat taattaccgc ctcttgctcc acatttgccg cccctcaaca
    13261 aatcaacgat attgtgcatc gcacaattac cccgcttata gagcaacaaa ggatccccgg
    13321 tatggcggta gcggtaattt atcagggtaa accttattac tttacctggg gctatgcgga
    13381 catcgccaaa aagcagcccg tcacacagca aacgttgttt gagttaggtt cggtcagcaa
    13441 aacatttacg ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca agttaagcga
    13501 tcccacaaca aaatactggc ctgaacttac cgctaaacag tggaatggga tcacactatt
    13561 acatctcgca acctacaccg ctggcggcct gccattgcag gtgccggatg aggtgaaatc
    13621 ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct gcatgggctc caggaacaca
    13681 acgtctgtat gccaactcca gtatcggttt gttcggcgca ctggctgtga agccgtctgg
    13741 tttgagtttt gagcaggcga tgaaaactcg tgtcttccag ccactcaaac tcaatcatac
    13801 gtggattaat gtaccgtccg cagaagaaaa gaactacgcc tggggatatc gcgaaggtaa
    13861 ggcagtacat gtttcgccag gggcgttaga tgctgaagct tatggtgtga agtcgaccat
    13921 tgaagatatg gcccgctggg tgcaaagcaa tttaaaaccc cttgatatca atgagaaaat
    13981 acttcaacaa gggatacaac tggcacaatc tcgctactgg caaaccggcg atatgtatca
    14041 gggtctgggc tgggaaatgc tggactggcc ggtaaatcct gacatcatca ttaacggcag
    14101 tgacaataaa attgcactgg cagcacgccc cgtaaaaccg attacgcccc caactcctgc
    14161 agtacgcgca tcatgggtac ataaaacggg ggcgaccggc ggatttggta gctatgtcgc
    14221 gtttattcca gaaaaagagc tgggtatcgt gatgctggct aacaaaaact atcccaatcc
    14281 agcgagagtt accgccgcct ggcagattct caacactcta cagtaaaatt tcatcgggtc
    14341 cgaattttcg gaccttttct ccgcttttcc ttgctgtcat ctacacttag aaaaaaacca
    14401 gtaaggaaac atttatgcgc ctgctccctc tcgttgccgc agcgacagct gcatttctgg
    14461 tcgttgcctg cagttctcct acaccgccgc gtggcgtgac cgtagtgaat aatttcgacg
    14521 ctaaacgcta tctcggcacc tggtatgaga ttgcccgttt tgatcaccgc tttgaacgtg
    14581 gactggaaaa agtcaccgca acatacagcc tgcgtgatga cggcggcctg aatgtcatta
    14641 ataaaggcta taaccctgac agagaaatgt ggcagcagag tgaagggaaa gcgtacttta
    14701 ccggcgcacc aactcgcgct gcgctgaaag tgtcattctt tggtcctttc tatggcggtt
    14761 ataacgttat tgcactcgat cgggaatacc gccatgcgct ggtttgcggc ccggaccgcg
    14821 actacctgtg gatactctcc cgcacgccaa ccatttctga cgaagtgaaa caggagatgc
    14881 tggcagtcgc gacccgggaa gggtttgatg tcagtaagtt tatttgggta cagcagcccg
    14941 gtagttaatg agtgctgagt ttcagaccaa taatccccaa tacgattaac gccagactcg
    15001 ccagacgcat cgggttagcg gactcaccga gcaacacaat gccggtgatg gccgcgccga
    15061 ctgcgccaat acccgtccac acggcataag ccgtccctac tggtaacgat ttcatcgccc
    15121 aggcaagtag cgccatactg acaatcatcg ccgtcacggt aataacactt ggcgtcaaac
    15181 gactaaagcc gtgggtatat ttcaggccaa cggcccatac cacttccagc agaccagcaa
    15241 taactaagat aatccaggac atatcaggct ccggaaaatt ggggccgtcc ccggtgaaaa
    15301 gacgcgtttg caggtcgtcc tgcaaagctg atggttgaac ggatattttg ccaggtggca
    15361 ggggaatttc aactattacc tatgtaacta tcgcagaaaa aacaaacggc acgacacagc
    15421 tgtcgtaccg ttattgctta ttgctgcgct ttcgttgctg cgccagaaat cgcgttaccg
    15481 ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa tactgttgaa
    15541 agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc ttccttttag
    15601 gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc caaaaatgac
    15661 ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcgg gagatggagt
    15721 tgccgaggtg tttgatatct tcgccgaaac ctcgagcggt gttacagccc gtaagcagcg
    15781 tgctggcaag caaaacaaga acgataaggc gtttcatcat ctctaacctg aataagtgat
    15841 ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg tattcaccag
    15901 agcgggtatc cactttgatg acttcgccga tttgtacaaa cagcggaact ttaaccacag
    15961 cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct ttcaggcccg
    16021 gatcggtatc cacgatttcc agttcaacga agttcggcgg agtaacggag atcggctgac
    16081 cattccacag agttacgata cactctgcct gatccagcaa ccatttggcg ttgtcgccaa
    16141 ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag tgccagaact
    16201 caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcacct tcagcggaat
    16261 cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc agtttaacgc
    16321 gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc gcgtaaggtt
    16381 cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac gttgccataa
    16441 ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat tgtaacccta
    16501 aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac cgatcctgat
    16561 gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg acgcagcgcc
    16621 aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga gaaaggcaat
    16681 ccgaacgatc cgcttttgcg tcaggtaatt acctcgcaag atgagtttgt tgtcgcgccc
    16741 ggattctcca ccgacccgct ggaagaacag cacagcgtag tgccaggttt gttgcataaa
    16801 taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg ccgctattgc
    16861 ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg gcagactgca
    16921 cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg cggcgatccg
    16981 ctgatggcga aagatcacga gctggactgg ttgctcacac aactggaagc catcccgcat
    17041 atcaaacgtc tgcggattca cagccgtctg ccgattgtga tcccggcacg catcacagat
    17101 gcgctggttg aacgcttttc tcattctacg ctgcaaatct tgctggtgaa tcacattaac
    17161 catgccaatg aggtagatga aacattccgt caggcgatgg ctaagttgcg ccgggtaggc
    17221 gttactttgc tgaaccagag cgttctgtta cgtggtgtga acgataacgc gcaaacgctg
    17281 gcaaacctga gtaatgcgtt gttcgatgcg ggtgtgatgc cgtattacct gcatgtgctc
    17341 gataaagtac agggcgcggc gcattttatg gtgagtgatg acgaagcacg gcagattatg
    17401 cgtgagttgc tgacactggt gtcgggatat ctggtgccta aactggcgcg agaaattggc
    17461 ggcgaaccca gcaaaacacc gctggatctc cagctacgcc agcagtaact gattaaacag
    17521 ggaagtttaa tctttgcccg ttgcataacc tttaattatt catatacatt gctgttatta
    17581 ctatttatat aaccgacacc aataattaac ctttcttttc tgtttatata gagtgagcac
    17641 ggctatttat gttattttaa caactattac taccaagccc atttatctgg cttataaact
    17701 aaaattaatt atttaattga gaaatggtta gggagaacct acatggccat aagcatcaag
    17761 ggtgttaata ccggtgttat tcgcaagagt aataatttca ttgcactggc gctgaaaatt
    17821 aaagaacctc gcaataaaga atcgttgttc tttatgtctg ccatggaatt gcgcgacctt
    17881 ctgattgcgc tggaaagtcg tctgcatcaa aagcataaac tggatgctgc tgcacgcctt
    17941 cagtatgaac aagctcgcga taaagtcatt aagaaaatgg cagaaaatat cccagaaatt
    18001 ctggttgatg agcttaaaaa tgccgatatt aatcgtcggg ttaacacgct ggaacttacc
    18061 gatgatcagg gcgaaaatct taccttcgta ttaacactac atgacggtag caaatgtgag
    18121 ttagtcgtca atgagttgca gattgaaatg ctggcgcgag caatcattca tgcaattaat
    18181 aatgcggaaa tgcgtgaact ggcattacgt attacctcac tgttagattt cctgccgtta
    18241 tatgatgtcg actgccagga tgatggtaat ctggaatatg acacctattc ccaaccggaa
    18301 tggaaacata acttgttcaa ccattatctg gcggtgcttt accgctttaa agatgaaagt
    18361 ggcaaagaac aatttagcgg cgcagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa
    18421 gtggaggcga tcactcgccg aatgcttgat ttcagccccc gactgaaaaa actcgcgggt
    18481 gtgccatgtc aggtctatgt tcgcaccgta gcggcaaata acgcccaacc gctaacccag
    18541 gatcaatgct tacgcgcgct gcatcactta cgtgttcaat ccaccagcaa aactgcacca
    18601 caggcgaaat aatcttatct gttttactac gaggctggaa gtatccagcc tctggatcac
    18661 atcagttcgg gcacttataa acctgaccga taatctggct atccgtcggg acaaaactgg
    18721 acaacattcc ctgcgacggg ctgctgatgc cataaatcac gttaccgccc attgcagccg
    18781 cctggttgcg cagatcgttt gctgcgccgc gcatagaacc gccttcttct ccgtgttgcc
    18841 cggaaagcca gttgctttgc ttacctgttg cagtaccaat cagctggcac tctgcacctg
    18901 gttgctcgtc cacaatgcgt acactctgac cggcagcact caattcgttg ctggagctac
    18961 aacccgccat cagtagcgcg gcaccgacaa tccctgctaa gtattttacg tgcatgttat
    19021 tccccataat caatgagctg gacgccactc gcgtcgtccg tgtctgaatc ttatactaga
    19081 aagatgacca aaagaaaaac ccccagacat ttctgcccgg gggtttgttt atttctgcga
    19141 ggtgcagggc aattacatca tgccgcccat gccacccatg ccgcccatac cgccagcagc
    19201 gcctaagtca gctgcatcgt ttttcggcag gtcggtaacc atgcactcgg tggtgatcat
    19261 caggccagcc acagaagccg cgtactgcag agcagaacgg gttactttgg ttggatccag
    19321 gatacccata tcgatcatgt tgccgtattc ttcagttgct gcgttgtaac cgtagttgcc
    19381 gtcgccgcct ttaacggtgt tagcaacaac agacggttct tcgccgcagt tcaggacgat
    19441 ctgacgcagc ggagcttcca ttgcacgcag tgcaactttg atacccacgt tctggtcttc
    19501 gttctgacca cgcaggtcag ccagtttaga cgctacgcgg atcagcgcaa caccaccacc
    19561 agcaaccacg ccttcttcta ccgcagcacg ggtcgcgtgc agggcgtctt caacgcgtgc
    19621 ttttttctct ttcatttcaa cttcggtagc agcaccaact ttgataactg caacgccgcc
    19681 tgccagtttc gctacgcgct cctgcagttt ttcacggtcg tagtcagaag ttgcttcttc
    19741 aatctgctga cggatctgag caacacggcc ctggattgca gcttcttcac ccacgccatc
    19801 gatgatggtg gtggtgtctt tgttgatcac aacacgttta gcctgaccca ggtcttccag
    19861 ggttgctttt tccagctcca taccgatctc ttcagagatt acggtaccgc cagtcagggt
    19921 tgcgatatcc tgcagcatag ctttacgacg atcgccgaag cccggagctt taaccgcagc
    19981 aactttcacg atgccacgca tggtgttaac aaccagagtt gccagtgctt cgccttcaac
    20041 atcttcagcg atgatcagca gcggtttacc tgctttggca acagcttcca gaaccggcag
    20101 catttcgcgg atgttggaga ttttcttgtc agccagcagg atgaacgggc tttccagttc
    20161 tactgcgcca gtttccggct tgttgatgaa gtaaggagac aagtagccac ggtcgaactg
    20221 cataccttca accacgtcca gttcgtcctg cagaccggta ccgtcttcaa cggtgataac
    20281 gccttcttta ccgactttgt ccatcgcttc agcgatcagt ttacctacgg tttcgtcgga
    20341 gttagcagag atggtaccaa cctgagcaat cgctttagag tcagagcacg gtacggacag
    20401 cgctttcagt tcttcaactg cagcggtaac agctttgtcg ataccacgtt tcaggtccat
    20461 cgggttcatg cccgcagcaa cagctttcag accctcagtg atgatagcct gagccagtac
    20521 ggttgcagtg gtggtaccgt cgcctgcagc gtcattcgct ttagaggcaa cttctttcac
    20581 catctgcgca cccatatttt cgaacttgtc ttccagttcg atttcacgag caacggaaac
    20641 accatctttg gtgatggtcg gtgcaccgaa agatttatcc agaactacgt tacggccttt
    20701 aggaccgagg gtaactttca ctgcatctgc cagtacgttt acgccgcgca gcattttcac
    20761 acgagcgtcg ttaccgaatt ttacgtcttt agctgccatt atctttattc cttaaattcg
    20821 tatgttcagt gtcgtgcgcg gattacgctt caacaattgc cagaatgtcg ctttcggaca
    20881 tgatcaacac ttcttcattg tcgatcttct cagatttcac accgtagcca tcgttgaaaa
    20941 taacgatgtc gccaactttc acatccagcg gcttcacttc gccattttca aggatacggc
    21001 cattgccgac agccagcact tcgccgcggg tggatttagc cgctgcagag ccggtcagaa
    21061 cgatgccgcc agcagattta gtttcaactt ctttacgctt gacgatcacg cgatcatgca
    21121 atggacgaat attcattgat aactctcctt tgagaaagtc cgtatctgtt atgggtgacg
    21181 ccggagctta cgtggtttcc cggctggtga ccagagaaat ggggatgagg cttcgcccct
    21241 tcaaggggga aaaagaaaaa aaattctgat cagcacaaaa tcgggtgaaa accctgattc
    21301 acctcacatt tcatcgctat ttcttcatca ccgtcataag caaatctgat tgtgctacca
    21361 tcgaaaatct acgcagttgc ccaaaatttg ggcgcaatcg gacatcaacc catgagtgga
    21421 ctcaaacaag aactggggct ggcccagggc atcggcctgc tatcgacgtc attattaggc
    21481 actggcgtgt ttgccgttcc tgcgttagct gcgctagtag caggcaataa cagcctgtgg
    21541 gcgtggcccg ttttgattat cttagtgttc ccgattgcga ttgtgtttgc gattctgggt
    21601 cgccactatc ccagcgcagg cggcgtcgcg cacttcgtcg gtatggcgtt tggttcgcgg
    21661 cttgagcgag tcaccggctg gttgttttta tcggtcattc ccgtgggttt gcctgccgcg
    21721 ctacaaattg ctgccggatt cggtcaggca atgtttggct ggcatagctg gcaactgttg
    21781 ttggcagaac tcggtacgct ggcgctggtg tggtatatcg gtactcgcgg tgccagttcc
    21841 agtgctaatc tacaaacagt tattgccgga cttatcgtcg cactgattgt cgctatctgg
    21901 tgggcgggcg atatcaaacc tgcgaatatc cccttccctg cgccaggaaa tatc
//
LOCUS       DACADF010000473        17914 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.095, whole genome shotgun sequence.
ACCESSION   DACADF010000473 DACADF010000000
VERSION     DACADF010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.095"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            318..1634
                     /gene="fliD"
                     /locus_tag="ISB30_005097"
     CDS             318..1634
                     /gene="fliD"
                     /locus_tag="ISB30_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAP1992598.1"
                     /translation="MINPRTIAQEIAYADVATQATNLQEKQSELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ASGPAITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSSSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDAMKSDPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            1657..2049
                     /gene="fliS"
                     /locus_tag="ISB30_005098"
     CDS             1657..2049
                     /gene="fliS"
                     /locus_tag="ISB30_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAP1992599.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAR
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            2054..2365
                     /locus_tag="ISB30_005099"
     CDS             2054..2365
                     /locus_tag="ISB30_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HAP1992600.1"
                     /translation="MEKQRRQLFALVEAMNDALDKQRWRRLPALYQQLMRQFHDYAAA
                     ETDAAQLDAVKAQLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            2362..3423
                     /locus_tag="ISB30_005100"
     CDS             2362..3423
                     /locus_tag="ISB30_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HAP1992601.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPVFTLPKMVVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTSLPPQLQELIGQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTPPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLTV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            3431..3898
                     /locus_tag="ISB30_005101"
     CDS             3431..3898
                     /locus_tag="ISB30_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HAP1992602.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWSVWHHYAGKGKPTTAAQTES
                     VEALDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMTAPYKDLLVKKVVFQ"
     gene            3918..4634
                     /locus_tag="ISB30_005102"
     CDS             3918..4634
                     /locus_tag="ISB30_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HAP1992603.1"
                     /translation="MLQDVFESEEFAATLVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWQELAEEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            4647..5510
                     /gene="motA"
                     /locus_tag="ISB30_005103"
     CDS             4647..5510
                     /gene="motA"
                     /locus_tag="ISB30_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAP1992604.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            5513..6436
                     /gene="lafU"
                     /locus_tag="ISB30_005104"
     CDS             5513..6436
                     /gene="lafU"
                     /locus_tag="ISB30_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAP1992605.1"
                     /translation="MRKTGNHRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            6507..7562
                     /gene="dinB"
                     /locus_tag="ISB30_005105"
     CDS             6507..7562
                     /gene="dinB"
                     /locus_tag="ISB30_005105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP1992606.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPTFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            7559..8011
                     /locus_tag="ISB30_005106"
     CDS             7559..8011
                     /locus_tag="ISB30_005106"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP1992607.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            8189..9340
                     /locus_tag="ISB30_005107"
     CDS             8189..9340
                     /locus_tag="ISB30_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAP1992608.1"
                     /translation="MEWYMGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQ
                     RVVGMPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEK
                     RLSDLDDVAEESWLEENLPSAFAQHPWHNSLGSIGGGNHFAELQQIDQIIDAELFARA
                     GLDAQHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARI
                     NRQLIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGS
                     RGDYSWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICR
                     DKQLIFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            9337..9951
                     /locus_tag="ISB30_005108"
     CDS             9337..9951
                     /locus_tag="ISB30_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HAP1992609.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(10007..11464)
                     /gene="pepD"
                     /locus_tag="ISB30_005109"
     CDS             complement(10007..11464)
                     /gene="pepD"
                     /locus_tag="ISB30_005109"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAP1992610.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFNLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            11725..12183
                     /gene="gpt"
                     /locus_tag="ISB30_005110"
     CDS             11725..12183
                     /gene="gpt"
                     /locus_tag="ISB30_005110"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP1992611.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            12275..13519
                     /gene="frsA"
                     /locus_tag="ISB30_005111"
     CDS             12275..13519
                     /gene="frsA"
                     /locus_tag="ISB30_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAP1992612.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILEVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     ALRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            13577..13978
                     /locus_tag="ISB30_005112"
     CDS             13577..13978
                     /locus_tag="ISB30_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAP1992613.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(14017..15072)
                     /gene="phoE"
                     /locus_tag="ISB30_005113"
     CDS             complement(14017..15072)
                     /gene="phoE"
                     /locus_tag="ISB30_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAP1992614.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            15361..16464
                     /gene="proB"
                     /locus_tag="ISB30_005114"
     CDS             15361..16464
                     /gene="proB"
                     /locus_tag="ISB30_005114"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP1992615.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            16476..17729
                     /gene="proA"
                     /locus_tag="ISB30_005115"
     CDS             16476..17729
                     /gene="proA"
                     /locus_tag="ISB30_005115"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP1992616.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADARANGLSEAMLDRLTLTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALNSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADTAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT         4421 a         4460 c         4880 g         4153 t
ORIGIN      
        1 gattgccgga tgcggcgtga acgccttatc cggcctacaa atgggcacac gcataaaaac
       61 aggcgcggac tcgtaggcct gataagacgc gtcagcgttg catcaggcac tgtgcaccga
      121 tagccggatg cggcgtaaac gccttatccg gccacgaaca acaccgcctg tgggcggtgt
      181 tgtgctttct gcggcaggct tttttccgcc agaccggaaa cgtcatttcc gcctgttttt
      241 caatctattg attattcaca taaaaaatat tggcacgcct tttgccaata acctgacatt
      301 gttatccgga gtcatcaatg atcaacccaa gaaccattgc gcaagaaatc gcgtatgcgg
      361 atgtcgctac tcaggcgacg aatttgcagg agaagcagag tgagctggat gctgaaagca
      421 gcggcctgga ctcgctcagc tcggcgttga gcgatttcca gagcgccgtt gacgcgctga
      481 acagcgatac cgacggcccg gtgacttttg ccgccaccag caataatgac tcggcgaccg
      541 tttccgccaa ttcccaggcg caggcgggaa gctactcgtt tttcgttgag caactggcgc
      601 aggggcagca aaccacgttc agtatgggcg acgacgcctt ttccgctacc ggtacttttg
      661 aactgacgat gggcgacagc actatggata tcgatctgtc ggcggcagat cagaacggtg
      721 acggtgacgg ttttatcgac gccagcgagc tggtgaatgc gattaacgac tccgatgaca
      781 atccgggcgt gtcggcggca ctggtaaaaa ccgacggcac caccacgatt atgctcacct
      841 ccgatagcac cggggcgcag agcgcgtttt cggtgagcgt aacggggcat gatgccagca
      901 acgatagcac cagtgcgccc gttgccacgg atgtttcctc cgctcaggac gcgattattc
      961 atctcggtag cgccagcgga ccggctatca ccaacagcag caacacgttt gatgatgtga
     1021 tccccggtgt caccatgact tttaccgaag tcagcgattc tgacagcgat ctcaccacct
     1081 ttaacatcag tgaagattcc agcgccagcc aggagaaagt acagaccttt gtcgatgcct
     1141 ataacacctt gattgatacg gttgattcgc taaccaccca cggtgatgac agtagtagcg
     1201 ccggggtctt tgcgggggat gctggactca gctcactggc aaaccagctc gatgacatcg
     1261 cccatgccag ctacaacggc gtgtcgattg tcgactatgg catcacgctc gattctcacg
     1321 gccacttaca gattgactcc gaccagttca acgacgcgat gaagagcgat cccgacggcc
     1381 tgacctctat tttcgttggc gacaacagca tggtggcgca gatggacgac ctgattaaca
     1441 cctacacgga ctccagcaac ggcatcatca ccctgcgcca gcagaacatt gatgatcaga
     1501 tgagcaaaat tcaggacgaa ggcgatcagc ttaccgatac ctataacgct aactacgacc
     1561 gttatctcga ggagtacacc aatacgttgg ttgaggtgta caccatgaaa gccagtatgg
     1621 cggcatttgc gtaattcaga aggaatatcc ttaaagatgt accaagtaca ggacggttat
     1681 tcgcaataca aagagatcga tctcgcggcg cgtaccgcgg cggcatcgcc tcttgaactg
     1741 gtgctggtgc tgttttctgg cctgatggac gagctggaac gcgccagaag ccacattgaa
     1801 gggcggcgtt tcgagaagaa agcccagagc atcaataaat gcatcgacat tctcaatgcg
     1861 ctcaccagtt cgctggaatt tgaaaccggc ggcgagctgg tggtcaacct ttcgcgcctc
     1921 tatgaccact gtgtgtatcg cttgtatgaa gccagcggcg aactgtcggc agagaaaatc
     1981 gatgaagtga tgctgatctt aagcaacctg cgcgaaggct gggaagggct ttcgggcaag
     2041 ctgggttaag acgatggaaa aacaacgcag gcaacttttt gcactggtcg aagcgatgaa
     2101 tgacgccctc gacaagcaac gctggcgacg ccttccggca ctgtatcagc agttgatgcg
     2161 ccagtttcac gattacgcgg cggcggaaac cgacgcggcg cagttggatg cggtgaaggc
     2221 acaactgtac ggcgcgttta atgccctgat taaacggcgc gagcagcgcg cagaagtgtt
     2281 gaaagcgcgt atggaacagc atcagcggca tcaggaaggc gtactggcct actcaattgt
     2341 taatttactt tcggagaagt catgaatccg gcattgcttg ccacgctcgg aacgcttgcg
     2401 gaaaccgctt cgttgaaggc ggatatttta ccgccggtga gcggtgaaaa cgcgcccgtg
     2461 tttaccctgc cgaaaatggt ggtcgcggcg gtggcggagc gcgttcatag cgctaaaacc
     2521 agtcagcaac aggcgacgcg cccgcaggag aacgatccgg tggcgatgca ggcgctaatg
     2581 gcgctgttac ttccgcaacc tgccgcgccg catcaggaca cgccgcagcc gcgaaacgtc
     2641 gcaacatcgc cggttatcca gcaattgacg aaagcggtgg tgcaaaacgc gccgcaacgc
     2701 ccgacgcagc agcaggaact cacgtcgttg ccgccgcagt tgcaggaact gatcggtcag
     2761 ttgccgcagg cgaaacccga acagcaggcc agactggcga cttacgccag tgaagattta
     2821 catgccattg cgccgacgca gccgcgcgtc tcaacaccgc cagctcgccc gaaacctgaa
     2881 ctaacccgtg tgaccgcgcg cccgcaggtc gagcgtaaaa cggaaaaagt gccggacagc
     2941 gaaccggtta ttgcgcgtgc ggtgttgcag gttaagacgc cagagttggt cagcgatcat
     3001 caggagattg tcgccaaacc agccacgctt tcgatggacg aactgggcga aaaactgacg
     3061 acgctgttga aagatcagat ccactttcag ctcaacaaac aacagcagat ctccaccatt
     3121 cgtctcgatc caccgtcgct tggcaagctt gagatcgccg tacagttgga taacggcaaa
     3181 ctgacggtcc atatcggtgc gaatcaaagc gaagtctgcc gcgcattgca gcagtttagc
     3241 gacgatctcc gccagcatct gacggcgcaa aattttatgg aggtgaacgt acaggtttcc
     3301 tccgaagggc agtcgcagca gcaacaacag tcgggccatc agcaggaaga ggtgagtgcc
     3361 gccttacagc ttgatgatgc gcctcaattt caacagaacg aatccgtttt gatcaaagtg
     3421 taaaggcaat atgaagaaaa tcgtggtggc aggcgtggtc agcgccgttc tggcgttggt
     3481 ggtcggcgct ggagcaggat ggagtgtctg gcatcattat gccggaaaag gaaaaccgac
     3541 taccgccgcg caaacggaat cggtggaagc tctggatgag agcaagagcg tatttgtgac
     3601 gctgccggaa accattgtca ctttgcatga caacaacggc gcggatcatt atttgtcggc
     3661 ggaattagtg atggtggtcg ccagtgacaa agaggcggaa aaaataaagc atcaggaacc
     3721 gctgtatcaa agtattgcgg ttgaatgcct gacggaaatg aaatttgaag atttgcgcgg
     3781 catgaaaata tctgccatcc gcaaactgat ttctgacgcg ttaaagaaag atcttcagcg
     3841 tcgcaaaatg accgcaccgt ataaggattt gctggtgaaa aaagtggtct tccagtaatc
     3901 accaggcgga tataaacatg ctacaggatg tattcgagtc agaagaattt gcagccacgc
     3961 tggtattaac tccgcagcag gagagccact acttgcaggc ctatttaccg ttggtgcgta
     4021 aagtggtgcg gcaacttgcc ccgcagtgta attgcattat tgaccgtcag gatatggaac
     4081 aaacggcgtt aatggggctg ttgaacgcca ttcgtcgcta tgggctgccg gacgaaggtt
     4141 ttgcgggcta tgccgtgcac cgtattcgcg gtgcgatcct ggatgagctg cgcgctctcg
     4201 actggcgacc gcgccagctg cgacagaaat atcatcagat gaaagacctg attcgcgaaa
     4261 cgcgtaaaaa actggggcac gaaccggagt ggcaggagct ggctgaggaa gggatctcgc
     4321 atgaagatta tctcgaatat cagcaactgg aaggggcgga aacgctcgcc agcctggacg
     4381 aactgctgaa cggcgaaggg ccaggcattc cgcttgctgg ccgggaactg gaagatcagt
     4441 ttgtcactca gcaaatgttg caacacgcgc tttcacaatt aagcgaaaaa gagcaactga
     4501 tcctctccat gtattaccag catgagatga atctcaaaga gattgcgctg gtattagggc
     4561 taaccgaggc ccgaatttgt caactgaata aacagatcgc gcagaaaatt cgtgattttg
     4621 tctatccgaa ttaaagtgaa gaaatcatgc aaaaaatatt aggtttactg gtggtgctcg
     4681 gctgcgtatt tggcggttat tttgaagcgg gcgggcaatt agtggctatc tggcaaccgg
     4741 cggaaatgat cattattttg ggtgccggtt tcggggcgat gattattggt aacccgaaac
     4801 atgtgctgaa agagattgcc catcaaatta aaggcgtgat cagtaaaaaa cagctcgggc
     4861 cggagtttca gcgtcagcta ttaatgtgcc tgtatgagtt gctcgaaatg gtgcaaaacg
     4921 gcggtctgcg gatgttggat cagcatattg agcagccgga agagagcacc attttccaga
     4981 aatacccgct ggtgttaacc cagaagcggc tggtgacctt tattgctgat aactttcgcc
     5041 tgatggcgat gggtaaaatc gacgcccatg agctggaagg cattctcgat caggaactgg
     5101 acaccgcgga agagtccctg ttaacgccgt cgcgctcgct gcaacgtacc gccgaagcga
     5161 tgccgggatt tggtatttgc gcggcggtgc tgggcatcat catcaccatg cagtcgattg
     5221 atggatctat cgctctgatt ggcctgaaag tggcggcggc tctggtgggg acgtttttag
     5281 gcgtgtttat ctgctactgc ctgatggacc cgctagccaa cgccatggaa cagcaggcgc
     5341 gggcggaaca ttctttgctg gaatgcgtgc gcactgtgct ggtggcgcag gcaggcggca
     5401 aaccgacgct gctggcggtg gacgccgggc gtaagctgct gcatctggcg tccaaaccga
     5461 ccttcgccaa cctcgacgcg tgggtgaatg cgatgctgga gcaagaataa gcatgagaaa
     5521 gacgggcaac caccgcgaac gtggggcaaa aaccaccatc gtccggcggc agatcaaaaa
     5581 gaatcatgcc gggcatcacg gcggggcgtg gaaggtggcg ttcgccgact ttactttggc
     5641 aatgatggcg ctatttatga cgctgtggat tgtcaacagc gtcagtaaat ccgagcgtga
     5701 aagcattatc gccgcgctgc acgggcagtc gatttttaac ggtggtgggt tgtcgccgtt
     5761 aaataaaatc agcccgtctc atccgccaaa acctgccacc gtggcggtgc cggaagagac
     5821 ggagaaaaaa gcgcgcgacg tcaacgagaa aacggcgctg ctgaagaaga aatccgccac
     5881 cgagcttggc gagctggcaa ccagtatcaa taccatcgcc cgtgatgcgc atatggaagc
     5941 gaatctggag atggagattg ttccccaggg attacgcgtg ctgattaaag acgaccagaa
     6001 ccgcaatatg ttcgaacgcg gcagcgcgaa gattatgccg ttctttaaaa ctttgctggt
     6061 ggagcttgcg ccagtgttcg actcgctcga taacaaaatt attattaccg ggcataccga
     6121 tgcgatggcc tacaaaaaca atatctacaa caactggaac ctttcgggcg atcgcgcgct
     6181 ttcagctcgt cgggtgctgg aagaggccgg aatgccggaa gataaagtga tgcaggtaag
     6241 tgcaatggcg gaccagatgc tgctggatgc caaaaatccg caaagcgcgg gcaaccggcg
     6301 cattgagatt atggtgctga ccaaaagtgc gtccgatacg ctgtatcaat actttggtca
     6361 gcatggggat aaagtggtgc agccgctggt gcaaaagctg gataagcagc aggtgctttc
     6421 gcagcgaacg cgttaaatgc tgaatcttta cgcatttctc aaaccctgaa atcactgtat
     6481 actttaccag tattgagagg tgagcaatgc gtaaaatcat tcatgtggat atggactgct
     6541 ttttcgcagc ggtggagatg cgcgacaatc ccgccctgcg cgatatccct attgctattg
     6601 gcggcagccg cgaacgacgg ggggtgatca gcaccgccaa ttatcccgcg cgtaaatttg
     6661 gcgtacgtag cgctatgccg acagggatgg cgctcaaatt atgcccgcat ctcaccttgc
     6721 ttccagggcg ctttgacgcc tacaaagaag cctcaaatca tatccgcgaa attttctcgc
     6781 gctatacctc gcgcattgaa ccgttatcac tggatgaagc ctatctcgat gtcaccgata
     6841 gcgtccattg ccacggttct gcgaccctca tcgcccagga aatccgccag acgatcttca
     6901 acgagctgca actgacggcg tctgcgggcg tggcaccagt aaagtttctc gccaaaatcg
     6961 cctccgacat gaataaaccc aacggccagt ttgtgattac gccggcagaa gttccgacat
     7021 ttttacaaac cttaccgctg gcaaaaattc ccggcgtcgg caaagtctcg gcggcaaaac
     7081 tggaagcgat ggggctgcgg acctgcggtg atgtgcaaaa gtgtgatctg gtgatgctgc
     7141 ttaaacgctt tggcaaattt ggccgcattt tgtgggagcg tagtcagggc attgacgaac
     7201 gcgacgttaa cagcgaaagg ttgcgaaaat ccgtcggtgt agaacgtacg atggcggaag
     7261 atattcatca ctggtctgaa tgtgaagcga tcatcgagcg gctttaccct gaactggagc
     7321 gtcgtttggc aaaggtaaaa cctgatttgc tgattgcccg ccagggggtg aaattaaagt
     7381 tcgacgattt tcagcaaacc acccaggaac acgtctggcc gcggctgaat aaatctgatc
     7441 taatcgccac cgcgcgtaaa acctgggatg aacgccgcgg cgggcgcggt gtgcgactgg
     7501 tggggctgca tgtgacgttg cttgacccgc aaatggaaag acaactggtg ctgggattat
     7561 gaataacata caaataagaa actatcagcc tggtgatttt cagcaactat gcgctatttt
     7621 ccttcgagct gttacgatga cggcgagtca gcactattct ccacagcaaa ttgccgcctg
     7681 ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag tgcgggttgc
     7741 agttattaat gcacaaccgg ttggttttat tacccgcatt ggacattata tcgatatgtt
     7801 atttgttgaa ccggaataca cccgacgcgg agttgccagc gctttgttaa aaccattgat
     7861 taagtctgaa tccgaactta cggtggacgc cagcataacc gcaaaaccct tttttgaacg
     7921 ttatggtttt cagacagtta agcagcagcg cgttgaatgc cggggaacgt ggtttactaa
     7981 tttttatatg cgatataaac cgcaacatta aatccagctt gcaatgaaaa taacgcccgt
     8041 ctagtatgtg cgcgttttct tattccacaa actgcaagga ggtaaatcat gacaaatcct
     8101 ttatcaatga ctctttgcag acctttccag gattaattct tttttcttgc cctggattcg
     8161 tctgccattt cctgattttt attcataaat ggaatggtac atgggcaaat atattcgtcc
     8221 cttatccgat gcggtattta ccatcgcatc tgatgacctg tggatcgaga gtttagcgat
     8281 ccaacaatta cacaccacgg caaatttacc caacatgcag cgcgttgtgg ggatgccaga
     8341 tttacatccc ggacgcggct acccgattgg cgcagcgttc ttctctgttg gtcgttttta
     8401 cccggcactg gtcggcaatg atatcggctg cggcatggcg ctatggcaaa cagatattct
     8461 cgctcgcaaa tacaacgccg ataagtttga aaagcgatta tcagatctgg atgacgttgc
     8521 tgaagaaagc tggctggagg aaaacctgcc gtcagcattt gcacagcatc cgtggcacaa
     8581 ctcgcttggc tccatcggtg gcggtaacca cttcgcagaa ctgcaacaga ttgatcaaat
     8641 tatcgacgcg gaactgtttg cacgggctgg tctggatgcg cagcatctgc aactactggt
     8701 tcatagcggc tcgaggggat tggggcagtc tattttacag cggcatattg cctcgttttc
     8761 gcatcatggt ttgcctgaag gcagtgacga cgcgctacgg tatattgcgg aacatgacga
     8821 tgcgctggcg tttgcgcgta ttaatcgcca gctgatcgct ttgcgcataa tgcaacaagt
     8881 taaggccacc ggaagtccgg ttctggatgt ggcgcataac tttgttagcg cgtgccaaat
     8941 cggcgatcaa cagggctggt tgcatcgtaa aggcgccacg ccggatgaca acggtctggt
     9001 gattattccc ggttcacgcg gcgattactc ctggctggtt aaacctgttg cgaacgaaaa
     9061 aacgctccac tcgctggcgc atggggcggg gcgtaaatgg gggcgcaccg agtgtaaagg
     9121 gcgtctggca gcgaaataca cggcgacgca actctcgcgg actgaacttg gcagtcgggt
     9181 aatttgtcgc gataaacaac tcatctttga agaagcgcca caagcctata aatcggctga
     9241 aagcgtggtg caatgtctgg tgctggctgg gttaattatt cctgtcgcgc gactgcgccc
     9301 ggtgctgacg ctcaaaaaca gtggagggaa aaaaggatga tcttgctaca actctcctct
     9361 gctcaggggc cggaagaatg ttgtctcgca gtgaaaaaag cactggacag gctgattaaa
     9421 gaagctaccc gacaggacgt cgcggtaacg gtgctggaaa cagaaacggg tcgctactct
     9481 gacacactgc gttcggcgct gatttctctg gatggcgata acgcatgggc tctaagcgaa
     9541 agctggtgcg gcactattca gtggatttgt ccgagtccgt atcggcctca tcatgggcgc
     9601 aaaaactggt ttctgggcat tgggcgtttt accgctgatg agcaggaaca atcggatgca
     9661 atccgttatg agacgctgcg ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac
     9721 tcggcggtac gcgccacgca tctggcatcc ggtattagcg tgaaggttca gtcagagcgc
     9781 agtcagcatg ctaacaaacg gctggcgcga ttactgattg cctggaagct ggaacaacag
     9841 caacaggaaa atagcgcggc gctgaaatcg cagcggcgaa tgttccatca ccagattgaa
     9901 cgtggcaacc cgcgacgaac gttcacaggg atggctttta tcgaaggata atgaaggatg
     9961 aaactgccgg aaggcgttaa acgccatccg gcagcaaatc aaataattac ttcgccggaa
    10021 tttctttcag cagttcagtc agcagtgtcc agtaatggcc aacgctttcg atatgaactt
    10081 gctcatccgg ggagtgtgga ccggtgatgg ttggcccgat agacaccatg tccatttccg
    10141 gatacggttt tttgaacaga ccacattcca ggcccgcgtg gataatctgg atgttcggcg
    10201 tcttgttgaa cagacgctga taggtttcac gtaccagatg cattaccgga gagttagcgt
    10261 ccggctgcca gccaggatat gcgccttttg cttcggtttt cgcacctgcc agtttaccca
    10321 gcgaatccag catgctcact acgtagtctt taccgctgtc gatcagagaa cggatcaggc
    10381 agtgaatttc tacgttattg tcggtcatgg tcaccacacc gacgttcagg gaggtttcaa
    10441 ccacgccttt ggctacatcg gaattacgga taactccgtt cggggtggcg ttcagcagac
    10501 gaataaaggt atcgcgagat gtcgccgtta atgcagcttt gtcgttcgct acggagtcca
    10561 gcagcaatgc cagattcttc tctttttctg ccagttcgtt tttcaggatc tcctgatagg
    10621 tatccaccag agatttcagg gcgtcgactt tatcagctgc gacagcaatg gtcgcaaagg
    10681 cttcacgcgg gatggcgtta cgcagtgtgc cgccgttgaa atcaataaga cgcaggtcca
    10741 gttcttctgc atgacccgcc aggaagcgca ccagcagttt gttggcatta cccaggccaa
    10801 cgtggatttc accgccggag tgaccgcctt tcagaccttt taaggttaaa ttgaaggttt
    10861 caaaaccagc tggaaccgct tcacgatcta aatgcaggtt ggaggtgaag tcgatacccc
    10921 ccgcacatcc catgtagatt tcaccttctt cttcggagtc ggtgttaatc agaatatcag
    10981 cctgcaacca gttgctctgt aagccgaacg caccgtccat accggcttct tcagtcatgg
    11041 tcagcagcac ttccagcggg ccgtgaacca cgttttcgtc agccagaacc gccagtgcgg
    11101 aggccatacc aatgccgtta tccgcaccca gcgtggtgcc gcgcgcttta acccattcgc
    11161 catcaatata aggctggata ggatctttag tgaagtcatg cacggtgtcg ttattttttt
    11221 gcggcaccat atcgaggtgg gcctgcaaga cgaccggttt acgattttcc atacctgcgg
    11281 tagcaggttt acgaatcagg atattaccta cctgatcgcg ttcgacatgg aaacctttct
    11341 cttttgccca accaacaatg tattcagcga gttgctcttc atgataggac gggtgaggaa
    11401 tagaacagat tttggcaaaa atatcccaca gcggctgtgg agataattga gacagttcag
    11461 acacgttaag tctccttgtc gatcacccgc aaaacagtat tgcaggtcac agggttagca
    11521 gaaaatgttg tcaacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
    11581 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
    11641 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
    11701 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
    11761 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
    11821 attgccgtaa gccgtggcgg tctggtaccg ggtgcgttac tggcgcgtga actgggtatt
    11881 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
    11941 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtagat
    12001 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
    12061 ttcgcaaaac cggctggtcg tccgctggtt gatgattatg ttgttgatat cccgcaaaat
    12121 acctggatcg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
    12181 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
    12241 atagtttcca gtaagtattc tggaggctgc atctatgaca caggcaaacc tgagcgaaac
    12301 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttagtca
    12361 cggcgcacaa ctgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
    12421 catgattaac cgtctgatgt ggatctggcg cggcattgac ccccgtgaaa tcctcgaagt
    12481 ccaggcgcgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
    12541 gattggctac cgcggcggca actggattta tgagtgggct acccaggcga tggtgtggca
    12601 acaaaaagcc tgtgcggaag aagatccgca actcagcggt cgacactggc tgcatgcggc
    12661 tacgttgtac aacattgctg cctatcctca tctgaaagga gatgacctgg ccgagcaagc
    12721 gcaggctttg tcaaaccgcg cctatgaaga ggccgctcag cgtctgccgg gcacgatgcg
    12781 gcagatggag tttaccgtac ccggcggtgc gcccatcacc ggctttttgc atatgccgaa
    12841 aggcgatggc ccgttcccga cagtattaat gtgtggtggt ctggatgcga tgcagacaga
    12901 ctattacagc ctgtatgaac gttattttgc gctgcgcggc attgcgatgc tgactattga
    12961 tatgccgtcg gtgggctttt cttcaaaatg gaagctcacc caggactcca gcctgttgca
    13021 tcagcacgtt ttaaaggcgt tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc
    13081 ctttggtttc cgtttcggcg ctaacgttgc cgtacgtctg gcataccttg aatcgccgcg
    13141 tctgaaagcg gttgcctgtc ttggtccggt agttcatacc ctgttgagtg attttaagtg
    13201 ccagcaacag gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg ggatgcatga
    13261 tgcttctgac gaagcgttgc gcgtggagct gaatcgctat tcattaaaag tgcaagggtt
    13321 gctgggacgt cgctgcccaa cgccaatgtt atcaggctac tggaagaacg atccgttcag
    13381 cccggaagag gactcacgct taatcacgtc gtcatctgct gacggtaaat tattagagat
    13441 cccatttaac ccggtgtatc ggaattttga caaaggcctt caggaaatca cagactggat
    13501 cgaaaaacgt ttgtgttaaa aatttgctaa attttaccaa tttggtaaaa cagttgcatc
    13561 acaacaggag attgcaatga cgttaccgag tggacacccg aagagcagat tagtcaaaaa
    13621 atttaccgca ctaggcccgt atattcgtga aggtaagtgc gaagataatc gattcttttt
    13681 cgattgtctg gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg agttctgggg
    13741 ctggtggatg gaactggaag cgcaggaatc ccgttttacc tacagttacc agtttggtct
    13801 gttcgataaa gcaggcgact ggacgagtgt tcagataaaa gatgccgaag tggttgaacg
    13861 actggagcac accctgcgtg aatttcacga gaagctgcgt gagctgctgg cgacgctgaa
    13921 tctgaagctg gaaccggcag atgattttcg tgatgaaccg gtgaagttaa cggcgtgaat
    13981 gaaatgtgcc ggatgcatca catccggcaa tatccattaa aactgatagg tcatgccaac
    14041 cgcgacaata tcatcattat taatattcaa tttgttatcg ctatccagtt ggttgatttt
    14101 ataatcaaca aacgctgaca tatttttgtt gaaataatac gtagcaccaa catcgacata
    14161 attgaccaga tcttcatcac cgatgccctc aatatctttc cctttcgata agacataacc
    14221 cagcgatgga cgcagaccaa agtcaaactg gtattgagcg accgcttcaa agttctgcgt
    14281 cttattggca aagccgccag agattggcgt cattttacgt gtttcagaat agaaagttgc
    14341 cagataaata ttattggcat cgtatttcag acctgttgcc catgcttctg cacgcttgcc
    14401 tgtgccacgg ctttgcaggt tctgctcgtt ggtgcgatca gagttggtat aggctccact
    14461 gatggcgaaa tcgctgccac caaagtcata tgtcaatgac gtgccgaagc catcgccgtt
    14521 ttgcttttta acgtcgcggt tttcgttttt cccttgatat tgcagggtta agttcaggcc
    14581 atcgataacg ccgaagaagt cggtgttccg atacgtcgcc agaccgctgg cgcgtttggt
    14641 cataaagttg tcggtctgcg ccgaggagtc gccaccaaat tccgggaaca tatcggtcca
    14701 ggcttccacg tcatacaacg cgcccaggtt acgaccataa tcgaaagaac ccaaatcttt
    14761 atatttcaac ccggcaaaag cgagacgcgt tttttgctgt gcagtatcac tctccgcttt
    14821 attaccggca aactcagctt cccaacgacc ataaccagtc agttgatcgt taatttgtgt
    14881 ttcgccttta aaaccaaaac ggatataact ctggtcgcca tctttactgt cattatcact
    14941 catataatgc atggctttaa ctttgccata gacatccagt ttattaccgt ctttgttata
    15001 tatttctgcg gcctgtacgg atacagatgc cacaatgccc atcaccacta atgccagagt
    15061 gctctttttc attttcattc ctgattttaa ttaacgcgcg aatattcagc gggagagtcc
    15121 cgttgaaaac aggaaagttt ttaacctgag attgttaaag atatattaca gattaataat
    15181 attcttaaaa tgtggtaatt tattaaatct gtaataaaag cgtaaacaac tgccgctacg
    15241 cttgctgatc ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga
    15301 cagtcctgct aaaacgttcg tttgatatca ttttttccta aaattgaatg gcagagaatc
    15361 atgagtgaca gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac aggcggatcg
    15421 cgccgcctga accgtgccca tatcgttgaa cttgttcgcc agtgcgcgca gttacatgcc
    15481 gccgggcatc ggattgttat tgtgacgtcg ggtgcgattg ccgccggacg tgagcacctg
    15541 gggtacccgg aattgccagc gactatcgct tcaaaacagc tgctggcggc ggtagggcag
    15601 agtcgactga ttcaactgtg ggaacagctg ttttcaattt acggcattca tgtcgggcag
    15661 atgttgctga cccgtgctga tatggaagat cgtgaacgct tcctgaacgc ccgcgatacc
    15721 ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatta atgagaacga tgctgtcgct
    15781 acggcagaga ttaaggtcgg tgataacgat aacctttctg cgctggcggc gattctggcg
    15841 ggtgccgata aactgttgct gctgaccgat caaaaaggtt tgtataccgc tgacccgcgc
    15901 agcaatccgc aggcagaact gattaaagat gtgtacggca ttgatgacgc actgcgcgcg
    15961 attgccggtg atagcgtttc aggcctcgga actggcggta tgagcaccaa attgcaggcc
    16021 gctgacgtgg cttgccgtgc gggtatcgac accattattg ccgcgggcag caagccgggc
    16081 gttattggtg atgtgatgga aggcatttcc gtcggtacgc tgttccatgc ccaggcgact
    16141 ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc ctgcgggtga aatcacggta
    16201 gatgaagggg caactgccgc cattctggaa cgcggcagct ccctgttgcc gaaaggcatt
    16261 aaaagcgtga ctggcaattt ctcgcgtggt gaagtcatcc gtatttgcaa cctcgaaggt
    16321 cgcgatatcg cccacggcgt cagtcgttac aacagcgatg cattacgccg tattgccgga
    16381 caccactcgc aagaaattga tgcaatactg ggatatgaat acggcccggt tgccgttcac
    16441 cgtgatgaca tgattacccg ttaaggagct ggccgatgct ggaacaaatg ggcattgccg
    16501 cgaagcaagc ctcgtataaa ttagcgcaac tctccagccg cgaaaaaaat cgtgtgctgg
    16561 aaaaaatcgc cgatgaactg gaagcacaaa gcgaaagtat cctcaacgct aacgcgcagg
    16621 atgttgcaga cgcgcgtgcc aatggcctta gcgaagcgat gcttgaccgt ctgacactga
    16681 cgcccgcacg gctgaaaggc attgccgatg atgtgcgcca ggtgtgcaac ctcgccgatc
    16741 cggtggggca ggtaatcgat ggcggcgtac tggacagcgg cctgcgtctt gagcgtcgtc
    16801 gcgtaccgct gggggttatt ggcgtgattt atgaagcgcg cccgaacgtg acggttgatg
    16861 tcgcttcgct gtgcctgaaa acaggtaacg cagtgatcct gcgcggcggc aaagaaacgt
    16921 gtcgcactaa cgctgcaacg gtggcggtga ttcaggacgc cctgaactct tgtggcttac
    16981 cggcgggtgc cgtgcaggcg attgataatc ctgaccgtgc gctggtcagt gaaatgctgc
    17041 gtatggataa atacatcgac atgctgatcc cgcgcggcgg ggctggtttg cataaactgt
    17101 gccgcgagca gtcgacaatc ccggtgatca caggcggtat aggcgtatgc catatttacg
    17161 ttgatgaaag cgcagagatc gctgaagccc tgaaagtaat tgtcaacgcg aaaactcagc
    17221 gtccgagcac gtgtaatacg gtagaaacgt tgctggtgaa taaaaacatc gctgatagct
    17281 tcctgcccgc attaagcaaa caaatggcgg aaagcggcgt gacgttacac gctgatacag
    17341 ccgcgctggc gcagttgcag gcaggccccg cgaaggtggt ggctgttaaa gccgaagagt
    17401 atgacgatga gtttctgtca ttagatttga acgtcaaaat cgttagcgat ctggatgacg
    17461 ccattgccca tattcgtgaa cacggcacgc aacactccga tgcgatcctg acccgcgata
    17521 tgcgcaacgc ccagcgtttt gttaacgaag tggattcctc cgctgtttac gttaacgcct
    17581 ctacgcgttt taccgacggc ggccagtttg gactgggggc ggaagtggcg gtaagcacac
    17641 aaaaactcca cgcgcgcggc ccaatggggc tggaagcact gaccacttac aagtggatcg
    17701 gcattggtga ttacaccatt cgtgcgtaaa taaaaccggg tgatgcaaaa gtagccattt
    17761 gattcacaag gccattgacg catcgcccgg ttagttttaa ccttgtccac cgtgattcac
    17821 gttcgtgaac atgtcctttc agggccgata tagctcagtt ggtagagcag cgcattcgta
    17881 atgcgaaggt cgtaggttcg actcctatta tcgg
//
LOCUS       DACADF010000474         4190 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.254, whole genome shotgun sequence.
ACCESSION   DACADF010000474 DACADF010000000
VERSION     DACADF010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.254"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            108..1013
                     /gene="mph(A)"
                     /locus_tag="ISB30_005116"
     CDS             108..1013
                     /gene="mph(A)"
                     /locus_tag="ISB30_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HAP1992617.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1010..2248
                     /locus_tag="ISB30_005117"
     CDS             1010..2248
                     /locus_tag="ISB30_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP1992618.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2248..2832
                     /locus_tag="ISB30_005118"
     CDS             2248..2832
                     /locus_tag="ISB30_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP1992619.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3325..4089)
                     /locus_tag="ISB30_005119"
     CDS             complement(3325..4089)
                     /locus_tag="ISB30_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002008502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HAP1992620.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
BASE COUNT          712 a         1306 c         1305 g          867 t
ORIGIN      
        1 acaaaaggct gcctcatcgc taactttgca acagtgccgg attgaatata accgacgtga
       61 ctgttacatt taggtggcta aacccgtcaa gccctcagga gtgaatcatg accgtagtca
      121 cgaccgccga tacctcccaa ctgtacgcac ttgcagcccg acatgggctc aagctccatg
      181 gcccgctgac tgtcaatgag cttgggctcg actataggat cgtgatcgcc accgtcgacg
      241 atggacgtcg gtgggtgctg cgcatcccgc gccgagccga ggtaagcgcg aaggtcgaac
      301 cagaggcgcg ggtgctggca atgctcaaga atcgcctgcc gttcgcggtg ccggactggc
      361 gcgtggccaa cgccgagctc gttgcctatc ccatgctcga agactcgact gcgatggtca
      421 tccagcctgg ttcgtccacg cccgactggg tcgtgccgca ggactcggag gtcttcgcgg
      481 agagcttcgc gaccgcgctc gccgccctgc atgccgtccc catttccgcc gccgtggatg
      541 cggggatgct catccgtaca ccgacgcagg cccgtcagaa ggtggccgac gacgttgacc
      601 gcgtccgacg cgagttcgtg gtgaacgaca agcgcctcca ccggtggcag cgctggctcg
      661 acgacgattc gtcgtggcca gatttctccg tggtggtgca tggcgatctc tacgtgggcc
      721 atgtgctcat cgacaacacg gagcgcgtca gcgggatgat cgactggagc gaggcccgcg
      781 ttgatgaccc tgccatcgac atggccgcgc accttatggt ctttggtgaa gaggggctcg
      841 cgaagctcct cctcacgtat gaagcggccg gtggccgggt gtggccgcgg ctcgcccacc
      901 acatcgcgga gcgccttgcg ttcggggcgg tcacctacgc actcttcgcc ctcgactcgg
      961 gtaacgaaga gtacctcgct gcggcgaagg cgcagctcgc cgcagcggaa tgagcgaacg
     1021 tcgatatagc ccgctcgcga cgctgttcgc ggcgaccttt ctcttccgga tcggcaacgc
     1081 ggtggcggcc ctcgcgcttc catggttcgt cctgtctcat acaaagagcg cggcctgggc
     1141 gggcgccacg gccgctagca gcgtcatcgc gaccatcatc ggcgcgtggg ttggtggtgg
     1201 cctcgtcgat cggttcgggc gcgcgcccgt cgcattgatc tcgggtgtgg tgggcggcgt
     1261 ggccatggcg agcatcccac tgctcgatgc cgttggcgcc ctctcgaaca ctgggctgat
     1321 cgcttgcgtg gtgctcggtg ccgcgttcga cgcacccggt atggccgcgc aggacagtga
     1381 gctgcccaaa ctcggccacg tcgccgggct ctccgttgag cgcgtctcgt cactgaaagc
     1441 ggtgatcggg aacgtcgcga ttctaggtgg cccggccctt gggggggccg caatcggcct
     1501 gcttggcgct gcgccaacgc tcgggctgac ggcgttctgc tccgtccttg caggtctgct
     1561 cggcgcgtgg gtgcttcccg cgcgtgccgc tcggacgatg accacgacgg cgactctctc
     1621 catgcgcgcc ggcgtcgctt ttctctggag cgaacccctg ctgcgccctc tctttggtat
     1681 agtgatgatc ttcgtgggca tcgttggcgc caacggcagc gtcatcatgc ctgcgctgtt
     1741 tgtagatgca ggacgccaag tagcagagct cgggctgttc tcctcaatga tgggggctgg
     1801 tggtctcctt ggcattgcca ttcatgcgtc ggtcggcgcc cggatatcag cgcagaactg
     1861 gctggcggtg gcattttgtg gctctgcggt gggctcgctt ctgctttcac agttgccagg
     1921 cgtgccggtg ctgatgttgt tgggcgcgct cgtgggactg ctgaccggct cagtctctcc
     1981 cattctcaac gctgccatct acaaccgcac gccgccagaa cttctcggcc gggtactcgg
     2041 cacggtctcg gcggtgatgc tgtcagcctc gcccatggtt atgcttgcgg ccggcgcgtt
     2101 tgtcgacctt gctggtccgc tccctggcct cgttgtatcg gccgtgtttg cggggctcgt
     2161 ggctctactc tcgctccgtc ttcaatttgc tacaatggcg gcggcagcca cagcctccgc
     2221 cccaacccat acagaaggtg aacactgatg ccccgcccca agctcaagtc cgatgacgag
     2281 gtactcgagg ccgccaccgt agtgctgaag cgttgcggtc ccatagagtt cacgctcagc
     2341 ggagtagcaa aggaggtggg gctctcccgc gcagcgttaa tccagcgctt caccaaccgc
     2401 gatacgctgc tggtgaggat gatggagcgc ggcgtcgagc aggtgcggca ttacctgaat
     2461 gcgataccga taggcgcagg gccgcaaggg ctctgggaat ttttgcaggt gctcgttcgg
     2521 agcatgaaca ctcgcaacga cttctcggtg aactatctca tctcctggta cgagctccag
     2581 gtgccggagc tacgcacgct tgcgatccag cggaaccgcg cggtggtgga ggggatccgc
     2641 aagcgactgc ccccaggtgc tcctgcggca gctgagttgc tcctgcactc ggtcatcgct
     2701 ggcgcgacga tgcagtgggc cgtcgatccg gatggtgagc tagctgatca tgtgctggct
     2761 cagatcgctg ccatcctgtg tttaatgttt cccgaacacg acgatttcca actcctccag
     2821 gcacatgcgt aaacggaggt gtgcagagtc cctgcggcag gcgacgaaca cgaccgtcgt
     2881 cgattagtac cggtacggtc ggtggtatcg aagtcttgat caccactcag gtctacggct
     2941 tacaaatggt gaccatcccg atacttgcgt cagagcaccg ggccgattct ttgacagtga
     3001 atcactcccg taaggttgtg ccggtgtggg tgtcccgggt cgagacgata ctccgccaat
     3061 gcgcccagca aacaacctgg ccatcgcagg tggtggggag cggtgtggcg gatgagttgg
     3121 acaagttggt gtagcagcac gagcacggcg agataacatc gcaggagttc gacatgctca
     3181 agagacagct gattgcgaat cgcgatgcag attcataacc cgattgcggg ttggcttcac
     3241 tccaccatca ccgagcagac tagcacggcg ggctctgttg caaagattgg cggcagtcag
     3301 aggtaggctg tcgctctgcg ccgatcaggc ggctgctgcg aaatggtggt tgagcatgcc
     3361 catggcctcc gtcagcgccg agggcccaat gccaaaagct ctctccacaa ggcgcacctc
     3421 gcccctgatg ccgggctgca ggcaccaggg gcgagcctgt cctttgcgca gggctcgcat
     3481 gacttcgaat cccttgatcg tggcataggc cgtggggatc gatttgaaac cgcgcaccgg
     3541 cttgatcagt atcttgagct ttccgtgatc ggcctcgatc acgttattga gatacttcac
     3601 ctgccggtgg gccgtctccc ggtccagctt tccttcgcgc ttcaattcgg tgatcgctgc
     3661 accatagctc ggcgctttgt cggtattgag cgtggcaggc ttttcccagt gcttcaggcc
     3721 tcgcagggcc ttgcccagga accgcttcgc tgccttggcg ctgcgggtcg gcgacaggta
     3781 gaaatcgatc gtgtcgcccc gcttgtcgac tgcccggtac aggtaggtcc acttgccccg
     3841 caccttgacg taggtttcat ccaggcgcca gctcggatca aagccacgcc gccagaacca
     3901 gcgcagccgc ttctccatct ccggggcgta gcactggacc cagcgataga tcgtcgtatg
     3961 gtcgaccgaa atgccgcgtt ccgccagcat ttcctcaagg tcgcgatagc tgatcggata
     4021 gcgacaatac cagcgcaccg cccacaggat cacatcaccc tggaaatggc gccacttgaa
     4081 atccgtcatc gttccgtccg tccaatctcc gccaagcatg ctcaagcttc acgatttttg
     4141 caacagagcc cacacgagta ttgagcatag tcgagattgg tgcagatcac
//
LOCUS       DACADF010000475         3360 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.274, whole genome shotgun sequence.
ACCESSION   DACADF010000475 DACADF010000000
VERSION     DACADF010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.274"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            complement(<1..142)
                     /locus_tag="ISB30_005120"
     CDS             complement(<1..142)
                     /locus_tag="ISB30_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992621.1"
                     /translation="MKVELGVAAGCNQAATAGIRGLTLSGTKTHVKKSFFNIIEAFIR
                     VIV"
     gene            159..1040
                     /locus_tag="ISB30_005121"
     CDS             159..1040
                     /locus_tag="ISB30_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-2"
                     /protein_id="HAP1992622.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSI
                     GVYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALV
                     PWSPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFR
                     LDRWELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNH
                     RIRAAVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKHSEAV
                     IAAAARLALEGLGVNGQ"
     gene            complement(1290..2171)
                     /locus_tag="ISB30_005122"
                     /pseudo
     CDS             complement(1290..2171)
                     /locus_tag="ISB30_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
     gene            complement(2275..2571)
                     /locus_tag="ISB30_005123"
     CDS             complement(2275..2571)
                     /locus_tag="ISB30_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor protein KorC"
                     /protein_id="HAP1992623.1"
                     /translation="MIRPETLRPFAEDWQAPTADEIKEVLELIRQRKGLSKPLSGVDV
                     ADLVGLPGERGSGKGTRTFRRWVSKTNPSPIAYGAWSILAHLAGFGAIWDADRD"
     gene            complement(2617..2772)
                     /locus_tag="ISB30_005124"
     CDS             complement(2617..2772)
                     /locus_tag="ISB30_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992624.1"
                     /translation="MNTDIVSLAKHIGDALTARRPVHMPRMKMRELGLLLIELKAYRE
                     AAATVTH"
     gene            complement(2900..3325)
                     /locus_tag="ISB30_005125"
     CDS             complement(2900..3325)
                     /locus_tag="ISB30_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAP1992625.1"
                     /translation="MMQTELNPLICSLVATPRRMAAMPRYVGRFYVVFESMLYQQMKG
                     LCREYRGAYWLMWELSNGGFYMAPGRRDEMLNIEAMNYFSGQMSADAAGITACLYLYS
                     HLSFHTEGADQERFSRLYHSLRDWACEHDEKEAILAAID"
BASE COUNT          659 a         1044 c          911 g          746 t
ORIGIN      
        1 agacaataac cctgataaat gcttcaataa tattgaaaaa tgacttctta acgtgagttt
       61 tcgttccact gagcgtcaga cccctaatcc cagctgtagc ggcctgatta catccggccg
      121 ctacacctag ctccaccttc aaacaaggaa tatcgttgat gtcactgtat cgccgtctag
      181 ttctgctgtc ttgtctctca tggccgctgg ctggcttttc tgccaccgcg ctgaccaacc
      241 tcgtcgcgga accattcgct aaactcgaac aggactttgg cggctccatc ggtgtgtacg
      301 cgatggatac cggctcaggc gcaactgtaa gttaccgcgc tgaggagcgc ttcccactgt
      361 gcagctcatt caagggcttt cttgctgccg ctgtgctggc tcgcagccag cagcaggccg
      421 gcttgctgga cacacccatc cgttacggca aaaatgcgct ggttccgtgg tcacccatct
      481 cggaaaaata tctgacaaca ggcatgacgg tggcggagct gtccgcggcc gccgtgcaat
      541 acagtgataa cgccgccgcc aatttgttgc tgaaggagtt gggcggcccg gccgggctga
      601 cggccttcat gcgctctatc ggcgatacca cgttccgtct ggaccgctgg gagctggagc
      661 tgaactccgc catcccaggc gatgcgcgcg atacctcatc gccgcgcgcc gtgacggaaa
      721 gcttacaaaa actgacactg ggctctgcac tggctgcgcc gcagcggcag cagtttgttg
      781 attggctaaa gggaaacacg accggcaacc accgcatccg cgcggcggtg ccggcagact
      841 gggcagtcgg agacaaaacc ggaacctgcg gagtgtatgg cacggcaaat gactatgccg
      901 tcgtctggcc cactgggcgc gcacctattg tgttggccgt ctacacccgg gcgcctaaca
      961 aggatgacaa gcacagcgag gccgtcatcg ccgctgcggc tagactcgcg ctcgagggat
     1021 tgggcgtcaa cgggcagtaa ggctctgaaa atcatctatt ggcccaccac cgccgccctt
     1081 gcgggcggca tggattacca accactgtca catttaggct aggagtctgc gcggcagagc
     1141 cgtgtgaccg gttttctgta gagcactgac gatggcggcg gcgctctctg caattggcaa
     1201 ggcgtcggcg ccaaggatac caatcttgcg gcgcgcggcg tgttatgacg actggggtgc
     1261 atttgagccg ccccatttaa ccttcgccct cacagatacg ccattcgcct cagatttagc
     1321 gccatgcaga cgagcttcca ctcggcttgc accttgtcca ggcccctcat gctgaactga
     1381 cgcaatccca tcaccgcctt gatccaacca ttcggagcct ccacgatcga cttgcgccgg
     1441 cggtaagctg catcgccttg ctccgttttc aatttcgccg caatcgccgc cgtatgcgga
     1501 tgggtcttgg cattgacctt ggcatcttca cgtccctcgc ggccgagggc aacgatgaca
     1561 tcgccgtggt gatcggcgac ctttgccaga acagcctcac tacggaatcc cgcatccgcc
     1621 agcgtctggg ccggcatttc tccggtgttg gcctgaactg ctgccagcat gcccagcagc
     1681 gcctgactgt ccgcggcgca gttggtcaac tccgccgcca cgatgatctg gtgctcggca
     1741 tcgaccgctg tgtacccgtt gtagctctgc tcggagccac caccggcgtg tttcatgatc
     1801 cggctgtccg gatcggtgaa gctttcctga tcacggtcat ccggcacacc aaactcgcgt
     1861 ttgtacgagc caccgccctt gtccgagcca tccggatggc gaggccggcg gccatcgtct
     1921 tcgctgcgcc cccgggcctg gtccgcttca cgctggcgcg cttccaggcg cgcctttgcc
     1981 gcctggatcg cctccaggcg cttctcgcgg cgagaaatct cggcaggaat gtccagctcc
     2041 ggctcgttac gctcctggtc gtcggtagcc ttggcgcgat caagcagcgc cttgatctcg
     2101 caatgcaatt cgtcctcggc cggcttcatg cgcttatagc tcatcgcctt gtggtcgcca
     2161 tgtttggcgg cttcgtattc aggttcttta gagagatcct ttagatcgcc gttcctcact
     2221 actgccggga agtctcccca gggagcacgt tcggccttat gttcggtcat ggtttcaatc
     2281 ccggtcagcg tcccatatag ccccgaaacc tgccaggtgg gccaagatag accaggcacc
     2341 ataagcaata gggctagggt tggttttgct cacccaacgc ctgaaggttc gggttccctt
     2401 tccgctgcct ctctcaccag gcaaaccaac gagatcagcg acatcaacgc cactgagagg
     2461 cttactaagc ccttttcgtt gcctaatcag ctccaacacc tctttgattt catcggcggt
     2521 tggagcctgc caatcttcag cgaacggcct aagcgtttca ggtctaatca tgcgataaat
     2581 cctcaaatag ccccggtcaa tgccggggct tttttatcaa tgggtaacgg tcgcggcggc
     2641 ctcccgatac gccttcagtt caatcaagag caagccaagc tcccgcatct tcatgcgcgg
     2701 catatgaacg gggcggcggg ccgtcagagc gtcgccaatg tgttttgcaa gtgatacaat
     2761 atccgtattc atcgcgttta tcctcaaatt gaccggtgaa agcccctgtg attggttgca
     2821 gccataccag gggcaccatt tctttttcta cctatgcacc tcctttaaga gccccggcca
     2881 tcaccggggc tttctgttgc tagtctattg cggccaagat ggcctctttt tcgtcatgct
     2941 cacaagccca atcccgcaag ctgtgataca gccttgaaaa ccgctcctgg tcagcgcctt
     3001 ctgtgtgaaa tgacagatgg ctatagaggt agaggcaagc ggtaatcccg gcagcatcgg
     3061 cgctcatttg gccactaaag tagttcatgg cctcgatgtt cagcatttca tcacgacgcc
     3121 caggagccat ataaaagccg ccgttcgaca gctcccacat aagccaataa gccccgcgat
     3181 actcgcggca gagccctttc atttgctggt aaagcatcga ctcgaaaacc acatagaaac
     3241 ggcctacata gcggggcatt gcagccatac ggcgaggggt ggcgaccagg gagcagatca
     3301 ggggattaag ttctgtttgc atcatcggag ttctccggta ttgaccgtgg cgggtgcccg
//
LOCUS       DACADF010000476         3225 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.277, whole genome shotgun sequence.
ACCESSION   DACADF010000476 DACADF010000000
VERSION     DACADF010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.277"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            86..322
                     /locus_tag="ISB30_005126"
     CDS             86..322
                     /locus_tag="ISB30_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAP1992626.1"
                     /translation="MAGQSLSVGFRPFPQTTPTRATAHERTPQQERQKAAREAERGRE
                     AWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(354..980)
                     /gene="tetR(A)"
                     /locus_tag="ISB30_005127"
     CDS             complement(354..980)
                     /gene="tetR(A)"
                     /locus_tag="ISB30_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAP1992627.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGGTVEQAPLS
                     PLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1086..2285
                     /gene="tet(A)"
                     /locus_tag="ISB30_005128"
     CDS             1086..2285
                     /gene="tet(A)"
                     /locus_tag="ISB30_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAP1992628.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2317..3177)
                     /locus_tag="ISB30_005129"
     CDS             complement(2317..3177)
                     /locus_tag="ISB30_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAP1992629.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
BASE COUNT          620 a         1011 c          984 g          610 t
ORIGIN      
        1 cgatcaagca ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg
       61 gaacacatca atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc
      121 cttcccgcaa acgaccccga cccgcgccac ggcccacgag cggacgccgc agcaggagcg
      181 ccagaaggcc gccagagagg ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat
      241 gaaaaagccc gtagcgggct gctacgggcg tctgacgcgg tggaaagggg gaggggatgt
      301 tgtctacatg gctctgctgt agtgagtggg ttgcgctccg gcagcggtcc tgatcaatcg
      361 tcaccctttc tcggtccttc aacgttcctg acaacgagcc tccttttcgc caatccatcg
      421 acaatcaccg cgagtccctg ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct
      481 atcgcggccc gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc ggcctgctcc
      541 tcaagcacgg ccccaacagt gaagtagctg attgtcatca gcgcattgac ggcgtccccg
      601 gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc catctgcggt
      661 gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc
      721 ctgaagctgc gggcattccc gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc
      781 gaatgcgtat gattctccgc cagcatggct tcggccagtg cgtcgagcag cgcccgcttg
      841 ttcctgaagt gccagtaaag cgccggctgc tgaaccccca accgttccgc cagtttgcgt
      901 gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca gggcggcacg gatcactgta
      961 ttcggctgca actttgtcat gcttgacact ttatcactga taaacataat atgtccacca
     1021 acttatcagt gataaagaat ccgcgcgttc aatcggacca gcggaggctg gtccggaggc
     1081 cagacgtgaa acccaacaga cccctgatcg taattctgag cactgtcgcg ctcgacgctg
     1141 tcggcatcgg cctgattatg ccggtgctgc cgggcctcct gcgcgatctg gttcactcga
     1201 acgacgtcac cgcccactat ggcattctgc tggcgctgta tgcgttgatg caatttgcct
     1261 gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg gcggccggtc ttgctcgtct
     1321 cgctggccgg cgctgctgtc gactacgcca tcatggcgac ggcgcctttc ctttgggttc
     1381 tctatatcgg gcggatcgtg gccggcatca ccggggcgac tggggcggta gccggcgctt
     1441 atattgccga tatcactgat ggcgatgagc gcgcgcggca cttcggcttc atgagcgcct
     1501 gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg gctgatgggc ggtttctccc
     1561 cccacgctcc gttcttcgcc gcggcagcct tgaacggcct caatttcctg acgggctgtt
     1621 tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt acgccgggag gctctcaacc
     1681 cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt cgccgccctg atggcggtct
     1741 tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt ttcggcgagg
     1801 atcgctttca ctgggacgcg accacgatcg gcatttcgct tgccgcattt ggcattctgc
     1861 attcactcgc ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc gaaaggcggg
     1921 cactcatgct cggaatgatt gccgacggca caggctacat cctgcttgcc ttcgcgacac
     1981 ggggatggat ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc ggaatgccgg
     2041 cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag ctgcaaggct
     2101 cactggcggc gctcaccagc ctgacctcga tcgtcggacc cctcctcttc acggcgatct
     2161 atgcggcttc tataacaacg tggaacgggt gggcatggat tgcaggcgct gccctctact
     2221 tgctctgcct gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa cgagccgatc
     2281 gctgatcgtg gaaacgatag gcctatgcca tgcgggtcaa ggcgacttcc ggcaagctat
     2341 acgcgcccta ggagtgcggt tggaacgttg gcccagccag atactcccga tcacgagcag
     2401 gacgccgatg atttgaagcg cactcagcgt ctgatccaag aacaaccatc ctagcaacac
     2461 ggcggtcccc gggctgagaa agcccagtaa ggaaacaact gtaggttcga gtcgcgagat
     2521 cccccggaac caaaggaagt aggttaaacc cgctccgatc aggccgagcc acgccaggcc
     2581 gagaacattg gttcctgtag gcatcgggat tggcggatca aacactaaag ctactggaac
     2641 gagcagaagt cctccggccg ccagttgcca ggcggtaaag gtgagcagag gcacgggagg
     2701 ttgccacttg cgggtcagca cggttccgaa cgccatggaa accgcccccg ccaggcccgc
     2761 tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc
     2821 agttccgcaa atagccccca ggaccgccat caatcgtatc gggctaccta gcagagcggc
     2881 agagatgaac acgaccatca gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg
     2941 caggcggtag accgaaataa acaacaagct ccagaatagc gaaatattaa gtgcgccgag
     3001 gatgaagatg cgcatccacc agattcccgt tggaatctgt cggacgatca tcacgagcaa
     3061 taaacccgcc ggcaacgccc gcagcatcgc gaccgtcatc ggtgagaagt tcggcaggta
     3121 ttgggtggtg acaatgtagg tgctgcccca aatggcaggt gctatcgctg tgaacaataa
     3181 atcggggcag acggcgcttc cagcgtgacg accatgccga gcacg
//
LOCUS       DACADF010000477         1597 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.336, whole genome shotgun sequence.
ACCESSION   DACADF010000477 DACADF010000000
VERSION     DACADF010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.336"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            112..459
                     /locus_tag="ISB30_005130"
     CDS             112..459
                     /locus_tag="ISB30_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HAP1992630.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            453..1292
                     /gene="sul1"
                     /locus_tag="ISB30_005131"
     CDS             453..1292
                     /gene="sul1"
                     /locus_tag="ISB30_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HAP1992631.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
BASE COUNT          303 a          462 c          469 g          363 t
ORIGIN      
        1 gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt
       61 tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat catgaaaggc
      121 tggctttttc ttgttatcgc aatagttggc gaagtaatcg caacatccgc attaaaatct
      181 agcgagggct ttactaagct tgccccttcc gccgttgtca taatcggtta tggcatcgca
      241 ttttattttc tttctctggt tctgaaatcc atccctgtcg gtgttgctta tgcagtctgg
      301 tcgggactcg gcgtcgtcat aattacagcc attgcctggt tgcttcatgg gcaaaagctt
      361 gatgcgtggg gctttgtagg tatggggctc ataattgctg cctttttgct cgcccgatcc
      421 ccatcgtgga agtcgctgcg gaggccgacg ccatggtgac ggtgttcggc attctgaatc
      481 tcaccgagga ctccttcttc gatgagagcc ggcggctaga ccccgccggc gctgtcaccg
      541 cggcgatcga aatgctgcga gtcggatcag acgtcgtgga tgtcggaccg gccgccagcc
      601 atccggacgc gaggcctgta tcgccggccg atgagatcag acgtattgcg ccgctcttag
      661 acgccctgtc cgatcagatg caccgtgttt caatcgacag cttccaaccg gaaacccagc
      721 gctatgcgct caagcgcggc gtgggctacc tgaacgatat ccaaggattt cctgaccctg
      781 cgctctatcc cgatattgct gaggcggact gcaggctggt ggttatgcac tcagcgcagc
      841 gggatggcat cgccacccgc accggtcacc ttcgacccga agacgcgctc gacgagattg
      901 tgcggttctt cgaggcgcgg gtttccgcct tgcgacggag cggggtcgct gccgaccggc
      961 tcatcctcga tccggggatg ggatttttct tgagccccgc accggaaaca tcgctgcacg
     1021 tgctgtcgaa ccttcaaaag ctgaagtcgg cgttggggct tccgctattg gtctcggtgt
     1081 cgcggaaatc cttcttgggc gccaccgttg gccttcctgt aaaggatctg ggtccagcga
     1141 gccttgcggc ggaacttcac gcgatcggca atggcgctga ctacgtccgc acccacgcgc
     1201 ctggagatct gcgaagcgca atcaccttct cggaaaccct cgcgaaattt cgcagtcgcg
     1261 acgccagaga ccgagggtta gatcatgcct agcattcacc ttccggccgc ccgctagcgg
     1321 accctggtca ggttccgcga aggtgggcgc agacatgctg ggctcgtcag gatcaaactg
     1381 cactatgagg cggcggttca taccgcgcca ggggagcgaa tggacagcga ggagcctccg
     1441 aacgttcggg tcgcctgctc gggtgatatc gacgaggttg tgcggctgat gcacgacgct
     1501 gcggcgtgga tgtccgccaa gggaacgccc gcctgggacg tcgcgcggat cgaccggaca
     1561 ttcgcggaga ccttcgtcct gagatccgag ctcctag
//
LOCUS       DACADF010000478         1585 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.337, whole genome shotgun sequence.
ACCESSION   DACADF010000478 DACADF010000000
VERSION     DACADF010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.337"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            59..532
                     /locus_tag="ISB30_005132"
     CDS             59..532
                     /locus_tag="ISB30_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HAP1992632.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            663..1451
                     /locus_tag="ISB30_005133"
     CDS             663..1451
                     /locus_tag="ISB30_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HAP1992633.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
BASE COUNT          392 a          392 c          405 g          396 t
ORIGIN      
        1 cgatgttacg cagcagggca gtcgccctaa aacaaagtta gccattaagg gagttaaatt
       61 gaaaatatca ttgatttctg cagtgtcaga aaatggcgta atcggtagtg gtcctgatat
      121 cccgtggtca gtaaaaggtg agcaactact ctttaaagcg ctcacatata atcaatggct
      181 ccttgtcgga agaaaaacat ttgactctat gggtgttctt ccaaatcgca aatatgcagt
      241 agtgtcaaag aacggaattt caagctcaaa tgaaaacgtc ctagtttttc cttcaataga
      301 aaatgctttg aaagagctat caaaagttac agatcatgta tatgtctctg gcgggggtca
      361 aatctataat agccttattg aaaaagcaga tataattcat ttgtctactg ttcacgttga
      421 agtcgaaggt gatatcaaat tccctataat gcctgagaat ttcaatttgg tttttgaaca
      481 gttttttatg tctaatataa attatacata ccagatttgg aaaaaaggct aacaatgcgt
      541 tgcagcacca gtcgcttcgc tccttggaca gcttttaagt cgcgtctttg tggttttgct
      601 gcgcaaaagt attccacaaa gccgcaactt aaaagctgcc gctgaactta acgttaggca
      661 tcatgggtga atttttccct gcacaagttt tcaagcagct gtcccacgct cgcgcggtga
      721 tcgagcgcca tctggctgcg acactggaca caatccacct gttcggatct gcgatcgatg
      781 gagggctgaa gccggacagc gacatagact tgctcgtgac cgtcagcgcc gcacctaacg
      841 attcgctccg gcaggcgcta atgctcgatt tgctgaaagt ctcatcaccg ccaggcgatg
      901 gcggaacatg gcgaccgctg gagctaactg ttgtcgctcg aagcgaagta gtgccttggc
      961 gctatccggc gcggcgtgag cttcagttcg gtgagtggct ccgccacgac atcctttccg
     1021 gaacgttcga gcctgccgtt ctggatcacg atcttgcgat tttgctgacc aaggcgaggc
     1081 aacacagcct tgcgcttcta ggcccatccg cagccacgtt tttcgagccg gtgccgaagg
     1141 agcatttctc caaggcgctt ttcgacacta ttgcccagtg gaatgcagag tcggattgga
     1201 agggtgacga gcggaacgtc gttcttgctc ttgctcgcat ttggtacagc gcttcaactg
     1261 gtctcattgc tcctaaggac gttgctgccg catgggtatc ggagcgtttg cctgccgagc
     1321 atcggcccct catctgcaag gcacgcgcgg cgtacctggg tagcgaggac gacgacctag
     1381 caatgcgcgt cgaagagacg gccgcgttcg ttcgatatgc caaagcaacg attgagagaa
     1441 tcttgcgttg agcggcatgt gcgaaaagtg catcgacccg cgccgagggc atctgatgcc
     1501 taactcggcg ttcaagcgga cgggctgcgc ccgccgctca actatgcgtt agatgcacta
     1561 agcacataat tgctcacagc caaac
//
LOCUS       DACADF010000479         1471 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.344, whole genome shotgun sequence.
ACCESSION   DACADF010000479 DACADF010000000
VERSION     DACADF010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.344"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..627
                     /gene="aph(3'')"
                     /locus_tag="ISB30_005134"
     CDS             <1..627
                     /gene="aph(3'')"
                     /locus_tag="ISB30_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP1992634.1"
                     /translation="GRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWP
                     SMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLA
                     RVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLAL
                     MIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            627..1463
                     /locus_tag="ISB30_005135"
     CDS             627..1463
                     /locus_tag="ISB30_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAP1992635.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          301 a          394 c          436 g          340 t
ORIGIN      
        1 ggtcgaggtg tggcttgccc cgaggtcatc aactggcagg aggaacagga gggtgcatgc
       61 ttggtgataa cggcaattcc gggagtaccg gcggctgatc tgtctggagc ggatttgctc
      121 aaagcgtggc cgtcaatggg gcagcaactt ggcgctgttc acagcctatc ggttgatcaa
      181 tgtccgtttg agcgcaggct gtcgcgaatg ttcggacgcg ccgttgatgt ggtgtcccgc
      241 aatgccgtca atcccgactt cttaccggac gaggacaaga gtacgccgca gctcgatctt
      301 ttggctcgtg tcgaacgaga gctaccggtg cggctcgacc aagagcgcac cgatatggtt
      361 gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac tcttcaatgc
      421 acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga tttggcactc
      481 atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg cgccttcgct
      541 gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc cttctatctg
      601 cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct gctcattggc
      661 acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg aaagtttcat
      721 tgccagacgg gactcctgca atcgtcaagg gattgaaacc tataaaagac attgctgatg
      781 aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc cggttgctcg
      841 gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc tctcacatcg
      901 ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg gcgaagctgt
      961 atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat cgctttgcag
     1021 ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac tacgtccacg
     1081 cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg ctacatggcg
     1141 atctgcatca tgaaaacatc atgttctcca gtcgcggctg gctggtgata gatcccgtcg
     1201 gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt ctacgatccg gctgacagag
     1261 acgacctttg tctcgatcct agacgcattg cacagatggc ggacgcattc tctcgtgcgc
     1321 tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc ttatgggtgc ctttccgcag
     1381 cttggaacgc ggatggagaa gaggagcaac gcgatctagc tatcgcggcc gcgatcaagc
     1441 aggtgcgaca gacgtcatac tagatatcaa g
//
LOCUS       DACADF010000480         1471 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.345, whole genome shotgun sequence.
ACCESSION   DACADF010000480 DACADF010000000
VERSION     DACADF010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.345"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            <1..627
                     /gene="aph(3'')"
                     /locus_tag="ISB30_005136"
     CDS             <1..627
                     /gene="aph(3'')"
                     /locus_tag="ISB30_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP1992636.1"
                     /translation="GRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWP
                     SMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLA
                     RVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLAL
                     MIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            627..1463
                     /locus_tag="ISB30_005137"
     CDS             627..1463
                     /locus_tag="ISB30_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAP1992637.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          300 a          394 c          437 g          340 t
ORIGIN      
        1 ggtcgaggtg tggcttgccc cgaggtcatc aactggcagg aggaacagga gggtgcatgc
       61 ttggtgataa cggcaattcc gggagtaccg gcggctgatc tgtctggagc ggatttgctc
      121 aaagcgtggc cgtcaatggg gcagcaactt ggcgctgttc acagcctatc ggttgatcaa
      181 tgtccgtttg agcgcaggct gtcgcgaatg ttcggacgcg ccgttgatgt ggtgtcccgc
      241 aatgccgtca atcccgactt cttaccggac gaggacaaga gtacgccgca gctcgatctt
      301 ttggctcgtg tcgaacgaga gctaccggtg cggctcgacc aagagcgcac cgatatggtt
      361 gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac tcttcaatgc
      421 acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga tttggcactc
      481 atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg cgccttcgct
      541 gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc cttctatctg
      601 cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct gctcattggc
      661 acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg aaagtttcat
      721 tgccagacgg gactcctgca atcgtcaagg gattgaaacc tatagaagac attgctgatg
      781 aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc cggttgctcg
      841 gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc tctcacatcg
      901 ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg gcgaagctgt
      961 atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat cgctttgcag
     1021 ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac tacgtccacg
     1081 cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg ctacatggcg
     1141 atctgcatca tgaaaacatc atgttctcca gtcgcggctg gctggtgata gatcccgtcg
     1201 gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt ctacgatccg gctgacagag
     1261 acgacctttg tctcgatcct agacgcattg cacagatggc ggacgcattc tctcgtgcgc
     1321 tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc ttatgggtgc ctttccgcag
     1381 cttggaacgc ggatggagaa gaggagcaac gcgatctagc tatcgcggcc gcgatcaagc
     1441 aggtgcgaca gacgtcatac tagatatcaa g
//
LOCUS       DACADF010000481         1136 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.365, whole genome shotgun sequence.
ACCESSION   DACADF010000481 DACADF010000000
VERSION     DACADF010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.365"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            75..935
                     /locus_tag="ISB30_005138"
     CDS             75..935
                     /locus_tag="ISB30_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-IId"
                     /protein_id="HAP1992638.1"
                     /translation="MHTRKAITEALQKLGVQTGDLLMVHASLKAIGPVEGGAETVVAA
                     LRSAVGPTGTVMGYASWDRSPYEETLNGARLDDEARRTWLPFDPATAGTYRGFGLLNQ
                     FLVQAPGARRSAHPDASMVAVGPLAETLTEPHELGHALGEGSPVERFVRLGGKALLLG
                     APLNSVTALHYAEAVADIPNKRWVTYEMPMLGRDGEVAWKTASDYDSNGILDCFAIEG
                     KPDAVETIANAYVKLGRHREGVVGFAQCYLFDAQDIVTFGVTYLEKHFGTTPIVPPHE
                     AVERSCEPSG"
     gene            948..>1136
                     /locus_tag="ISB30_005139"
     CDS             948..>1136
                     /locus_tag="ISB30_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tunicamycin resistance protein"
                     /protein_id="HAP1992639.1"
                     /translation="MIIWINGPFGAGKTTLAKRLRDRRSKSLIFDPEEIGFVVKETVP
                     MPASGDYQDLPLWRGLTIA"
BASE COUNT          230 a          313 c          355 g          238 t
ORIGIN      
        1 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtatagtgtt ttgcagttta
       61 gaggagatat cgcgatgcat acgcggaagg caataacgga ggcgcttcaa aaactcggag
      121 tccaaaccgg tgacctcttg atggtgcatg cctcacttaa agcgattggt ccggtcgaag
      181 gaggagcgga gacggtcgtt gccgcgttac gctccgcggt tgggccgact ggcactgtga
      241 tgggatacgc gtcgtgggac cgatcaccct acgaggagac tctgaatggc gctcggctgg
      301 atgacgaagc ccgccgtacc tggctgccgt tcgatcccgc aacagccggg acttaccgtg
      361 ggttcggcct gctgaatcaa tttctggttc aagcccccgg cgcgcggcgc agcgcgcacc
      421 ccgatgcatc gatggtcgcg gttggtccgc tggctgaaac gctgacggag cctcacgaac
      481 tcggtcacgc cttgggggaa ggatcgcccg tcgagcggtt cgttcgcctt ggcgggaagg
      541 ccctgctgtt gggtgcgccg ctaaactccg ttaccgcatt gcactacgcc gaggcggttg
      601 ccgatatccc caacaaacgg tgggtgacgt atgagatgcc gatgcttgga agagacggtg
      661 aagtcgcctg gaaaacggca tcggattacg attcaaacgg cattctcgat tgctttgcta
      721 tcgaaggaaa gccggatgcg gttgaaacta tagcaaatgc ttacgtgaag ctcggtcgcc
      781 atcgagaagg tgtcgtgggc tttgctcagt gctacctgtt cgacgcgcag gacatcgtga
      841 cgttcggcgt cacctatctt gagaagcatt tcggaaccac tccgatcgtg cctccgcacg
      901 aggccgtcga gcgctcttgc gagccttcag gttagaggcc gtcgacaatg ataatctgga
      961 tcaacggacc tttcggcgcc ggaaagacga cgctcgctaa gcggctgcgc gatcggcgtt
     1021 ccaaatcgct gatctttgac cccgaggaaa tcgggttcgt ggtgaaagaa acggtcccca
     1081 tgccagcgag cggagactat caggatctcc ccttgtggag gggacttacg atcgcg
//
LOCUS       DACADF010000482         1117 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.369, whole genome shotgun sequence.
ACCESSION   DACADF010000482 DACADF010000000
VERSION     DACADF010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.369"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            126..941
                     /locus_tag="ISB30_005140"
     CDS             126..941
                     /locus_tag="ISB30_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HAP1992640.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
BASE COUNT          298 a          231 c          257 g          331 t
ORIGIN      
        1 tgataagttt atcaccaccg actatttgca acagtgccct ctgatgttac attgcacaag
       61 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
      121 gtgttatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca
      181 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
      241 caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
      301 gtagcgttgc caatgatgtt acagatgaaa tggtcagact aaactggctg acggaattta
      361 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
      421 ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
      481 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
      541 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
      601 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
      661 ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt
      721 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
      781 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
      841 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
      901 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
      961 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcttcccta ccctcatggc
     1021 cgcctcccag gaatcgaccc aggtgcttat cgtggacata atcctttact cataaattgg
     1081 cactgttgca aagttagcga tgaggcagcc ttttgtc
//
LOCUS       DACADF010000483         1025 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.377, whole genome shotgun sequence.
ACCESSION   DACADF010000483 DACADF010000000
VERSION     DACADF010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.377"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            85..888
                     /locus_tag="ISB30_005141"
                     /note="aph(3'')-Ib"
     CDS             85..888
                     /locus_tag="ISB30_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAP1992641.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            888..>1025
                     /locus_tag="ISB30_005142"
     CDS             888..>1025
                     /locus_tag="ISB30_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP1992642.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PI"
BASE COUNT          212 a          266 c          287 g          260 t
ORIGIN      
        1 aaagaaaccg caagaattcg ttaactgcac attcgggata tttctctata ttcgcgcttc
       61 atcagaaaac tgaaggaacc tccattgaat cgaactaata ttttttttgg tgaatcgcat
      121 tctgactggt tgcctgtcag aggcggagaa tctggtgatt ttgtttttcg acgtggtgac
      181 gggcatgcct tcgcgaaaat cgcacctgct tcccgccgcg gtgagctcgc tggagagcgt
      241 gaccgcctca tttggctcaa aggtcgaggt gtggcttgcc ccgaggtcat caactggcag
      301 gaggaacagg agggtgcatg cttggtgata acggcaattc cgggagtacc ggcggctgat
      361 ctgtctggag cggatttgct caaagcgtgg ccgtcaatgg ggcagcaact tggcgctgtt
      421 cacagcctat cggttgatca atgtccgttt gagcgcaggc tgtcgcgaat gttcggacgc
      481 gccgttgatg tggtgtcccg caatgccgtc aatcccgact tcttaccgga cgaggacaag
      541 agtacgccgc agctcgatct tttggctcgt gtcgaacgag agctaccggt gcggctcgac
      601 caagagcgca ccgatatggt tgtttgccat ggtgatccct gcatgccgaa cttcatggtg
      661 gaccctaaaa ctcttcaatg cacgggtctg atcgaccttg ggcggctcgg aacagcagat
      721 cgctatgccg atttggcact catgattgct aacgccgaag agaactgggc agcgccagat
      781 gaagcagagc gcgccttcgc tgtcctattc aatgtattgg ggatcgaagc ccccgaccgc
      841 gaacgccttg ccttctatct gcgattggac cctctgactt ggggttgatg ttcatgccgc
      901 ctgtttttcc tgctcattgg cacgtttcgc aacctgttct cattgcggac accttttcca
      961 gcctcgtttg gaaagtttca ttgccagacg ggactcctgc aatcgtcaag ggattgaaac
     1021 ctata
//
LOCUS       DACADF010000484          961 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.382, whole genome shotgun sequence.
ACCESSION   DACADF010000484 DACADF010000000
VERSION     DACADF010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.382"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            49..909
                     /locus_tag="ISB30_005143"
     CDS             49..909
                     /locus_tag="ISB30_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAP1992643.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          263 a          217 c          235 g          246 t
ORIGIN      
        1 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
       61 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      121 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      181 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      241 ccaatgatga gcacttttaa agttctgcta tgtggtgcgg tattatcccg tgttgacgcc
      301 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      361 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      421 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
      481 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
      541 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
      601 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
      661 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
      721 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
      781 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
      841 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
      901 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      961 t
//
LOCUS       DACADF010000485          867 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.391, whole genome shotgun sequence.
ACCESSION   DACADF010000485 DACADF010000000
VERSION     DACADF010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.391"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            36..851
                     /gene="sul2"
                     /locus_tag="ISB30_005144"
     CDS             36..851
                     /gene="sul2"
                     /locus_tag="ISB30_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAP1992644.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIERIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          158 a          262 c          254 g          193 t
ORIGIN      
        1 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
       61 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
      121 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
      181 cggcatccag caaccccgac gccgcgcctg tttcgtccga cacagaaatc gagcgtatcg
      241 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
      301 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
      361 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
      421 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
      481 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
      541 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
      601 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
      661 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
      721 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
      781 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctaaaa gaaaccgcaa
      841 gaattcgtta actgcacatt cgggata
//
LOCUS       DACADF010000486          867 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.392, whole genome shotgun sequence.
ACCESSION   DACADF010000486 DACADF010000000
VERSION     DACADF010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.392"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            36..851
                     /gene="sul2"
                     /locus_tag="ISB30_005145"
     CDS             36..851
                     /gene="sul2"
                     /locus_tag="ISB30_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAP1992645.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIERIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          157 a          262 c          255 g          193 t
ORIGIN      
        1 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
       61 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
      121 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
      181 cggcatccag caaccccgac gccgcgcctg tttcgtccga cacagaaatc gagcgtatcg
      241 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
      301 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
      361 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
      421 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
      481 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
      541 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
      601 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
      661 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
      721 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
      781 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
      841 gaattcgtta actgcacatt cgggata
//
LOCUS       DACADF010000487          867 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.393, whole genome shotgun sequence.
ACCESSION   DACADF010000487 DACADF010000000
VERSION     DACADF010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.393"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            36..851
                     /gene="sul2"
                     /locus_tag="ISB30_005146"
     CDS             36..851
                     /gene="sul2"
                     /locus_tag="ISB30_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAP1992646.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          157 a          262 c          254 g          194 t
ORIGIN      
        1 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
       61 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
      121 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
      181 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
      241 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
      301 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
      361 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
      421 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
      481 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
      541 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
      601 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
      661 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
      721 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
      781 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctaaaa gaaaccgcaa
      841 gaattcgtta actgcacatt cgggata
//
LOCUS       DACADF010000488          867 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.394, whole genome shotgun sequence.
ACCESSION   DACADF010000488 DACADF010000000
VERSION     DACADF010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.394"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            36..851
                     /gene="sul2"
                     /locus_tag="ISB30_005147"
     CDS             36..851
                     /gene="sul2"
                     /locus_tag="ISB30_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAP1992647.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT          156 a          262 c          255 g          194 t
ORIGIN      
        1 actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg
       61 gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg gcgccagacg
      121 cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc gacctcggtc
      181 cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg
      241 cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac agttatcaac
      301 ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt
      361 ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc
      421 attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac atcatggatc
      481 acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca
      541 accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc gaaacctcgc
      601 tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt
      661 ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg
      721 ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc atccgcacac
      781 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
      841 gaattcgtta actgcacatt cgggata
//
LOCUS       DACADF010000489          642 bp    DNA     linear   BCT 02-NOV-2020
DEFINITION  TPA_asm: Escherichia coli isolate E02-6-1
            SAMN10735141-rid10686663.guided.427, whole genome shotgun sequence.
ACCESSION   DACADF010000489 DACADF010000000
VERSION     DACADF010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10735141
            Sequence Read Archive: SRR8436629
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-SEP-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000851645.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000851645.1
            Genome Coverage       :: 218x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/01/2020 04:38:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 5,148
            CDSs (total)                      :: 5,073
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 50 of 115
            Pseudo Genes (incomplete)         :: 66 of 115
            Pseudo Genes (internal stop)      :: 21 of 115
            Pseudo Genes (multiple problems)  :: 19 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10735141-rid10686663.guided.427"
                     /isolate="E02-6-1"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /country="China: Beijing"
                     /collection_date="2012-09-13"
     gene            57..530
                     /locus_tag="ISB30_005148"
     CDS             57..530
                     /locus_tag="ISB30_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA25"
                     /protein_id="HAP1992648.1"
                     /translation="MKVSLMAARAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGPLPNRKYAVVTRSNWTAANENVVVFPSIDEAMGRLGEITDHVIVAGGGEI
                     YHETIPMASTLHVSTIDVEPEGDVFFPNIPGKFDVVFEQQFTSNINYCYQIWQKG"
BASE COUNT          182 a          147 c          155 g          158 t
ORIGIN      
        1 cgatgttacg cagcagggca gtcgccctaa aacaaagtta acccaggacg agtaccttga
       61 aagtatcatt gatggctgca agagcgaaaa atggcgtaat cggttgcggt cctgacattc
      121 cttggtctgc caaaggggaa cagcttcttt tcaaagcact gacctataac caatggcttt
      181 tggtagggcg caaaacattt gagtctatgg ggccgctgcc caataggaaa tacgcggttg
      241 ttacccgctc aaactggaca gcggctaatg aaaacgtagt ggttttcccg tcgattgacg
      301 aagcgatggg tagattaggc gagatcactg accatgtcat cgtcgccggt ggtggagaaa
      361 tctaccatga aacgataccc atggcctcta ctctgcatgt gtcgacaatc gacgttgagc
      421 cagagggaga cgttttcttt ccgaacattc ctgggaagtt tgatgtcgtt tttgagcaac
      481 aatttacatc aaacattaac tattgctatc aaatctggca aaagggttaa caaagctatg
      541 caattgacgg caaaaagctt cgttcgcttc actcactacg caatttgccg caattgatag
      601 cggcgttaga tgcactaagc acataattgc tcacagccaa ac
//
